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Sample records for salvelinus namaycush brook

  1. Evidence of sound production by spawning lake trout (Salvelinus namaycush) in lakes Huron and Champlain

    Science.gov (United States)

    Johnson, Nicholas S.; Higgs, Dennis; Binder, Thomas R.; Marsden, J. Ellen; Buchinger, Tyler John; Brege, Linnea; Bruning, Tyler; Farha, Steve A.; Krueger, Charles C.

    2018-01-01

    Two sounds associated with spawning lake trout (Salvelinus namaycush) in lakes Huron and Champlain were characterized by comparing sound recordings to behavioral data collected using acoustic telemetry and video. These sounds were named growls and snaps, and were heard on lake trout spawning reefs, but not on a non-spawning reef, and were more common at night than during the day. Growls also occurred more often during the spawning period than the pre-spawning period, while the trend for snaps was reversed. In a laboratory flume, sounds occurred when male lake trout were displaying spawning behaviors; growls when males were quivering and parallel swimming, and snaps when males moved their jaw. Combining our results with the observation of possible sound production by spawning splake (Salvelinus fontinalis × Salvelinus namaycush hybrid), provides rare evidence for spawning-related sound production by a salmonid, or any other fish in the superorder Protacanthopterygii. Further characterization of these sounds could be useful for lake trout assessment, restoration, and control.

  2. Lake trout (Salvelinus namaycush) suppression for bull trout (Salvelinus confluentus) recovery in Flathead Lake, Montana, North America

    Science.gov (United States)

    Hansen, Michael J.; Hansen, Barry S; Beauchamp, David A.

    2016-01-01

    Non-native lake trout Salvelinus namaycush displaced native bull trout Salvelinus confluentus in Flathead Lake, Montana, USA, after 1984, when Mysis diluviana became abundant following its introduction in upstream lakes in 1968–1976. We developed a simulation model to determine the fishing mortality rate on lake trout that would enable bull trout recovery. Model simulations indicated that suppression of adult lake trout by 75% from current abundance would reduce predation on bull trout by 90%. Current removals of lake trout through incentivized fishing contests has not been sufficient to suppress lake trout abundance estimated by mark-recapture or indexed by stratified-random gill netting. In contrast, size structure, body condition, mortality, and maturity are changing consistent with a density-dependent reduction in lake trout abundance. Population modeling indicated total fishing effort would need to increase 3-fold to reduce adult lake trout population density by 75%. We conclude that increased fishing effort would suppress lake trout population density and predation on juvenile bull trout, and thereby enable higher abundance of adult bull trout in Flathead Lake and its tributaries.

  3. Use of cover habitat by bull trout Salvelinus confluentus and lake trout Salvelinus namaycush in a laboratory environment

    Science.gov (United States)

    Meeuwig, Michael H.; Guy, Christopher S.; Fredenberg, Wade A.

    2011-01-01

    Lacustrine-adfluvial bull trout, Salvelinus confluentus, migrate from spawning and rearing streams to lacustrine environments as early as age 0. Within lacustrine environments, cover habitat pro- vides refuge from potential predators and is a resource that is competed for if limiting. Competitive inter- actions between bull trout and other species could result in bull trout being displaced from cover habitat, and bull trout may lack evolutionary adaptations to compete with introduced species, such as lake trout, Salvelinus namaycush. A laboratory experiment was performed to examine habitat use and interactions for cover by juvenile (i.e., habitat, with bull trout using cover and bottom habitats more than lake trout. Habitat selection ratios indicated that bull trout avoided water column habitat in the presence of lake trout and that lake trout avoided bottom habitat. Intraspecific and interspecific agonistic interactions were infrequent, but approximately 10 times greater for intraspecific inter- actions between lake trout. Results from this study provide little evidence that juvenile bull trout and lake trout compete for cover, and that species-specific differences in habitat use and selection likely result in habitat partitioning between these species.

  4. If Arctic charr Salvelinus alpinus is “the most diverse vertebrate,” what is the lake charr Salvelinus namaycush?

    Science.gov (United States)

    Muir, Andrew M.; Hansen, Michael J.; Bronte, Charles R.; Krueger, Charles C.

    2016-01-01

    Teleost fishes are prominent vertebrate models of evolution, illustrated among old-world radiations by the Cichlidae of East African Great Lakes and new-world radiations by the circumpolar Arctic charr Salvelinus alpinus. Herein, we describe variation in lake charr S. namaycush morphology, life history, physiology, and ecology, as another example of radiation. The lake charr is restricted to northern North America, where it originated from glacial refugia and diversified in large lakes. Shallow and deepwater morphs arose in multiple lakes, with a large-bodied shallow-water ‘lean’ morph in shallow inshore depths, a small-bodied mid-water ‘humper’ morph on offshore shoals or banks, and a large-bodied deep-water ‘siscowet’ morph at depths > 100 m. Eye position, gape size, and gillraker length and spacing adapted for feeding on different-sized prey, with piscivorous morphs (leans and siscowets) reaching larger asymptotic size than invertivorous morphs (humpers). Lean morphs are light in color, whereas deepwater morphs are drab and dark, although the pattern is reversed in dark tannic lakes. Morphs shift from benthic to pelagic feeding at a length of 400–490-mm. Phenotypic differences in locomotion, buoyancy, and lipid metabolism evolved into different mechanisms for buoyancy regulation, with lean morphs relying on hydrodynamic lift and siscowet morphs relying on hydrostatic lift. We suggest that the Salvelinus genus, rather than the species S. alpinus, is a diverse genus that should be the subject of comparative studies of processes causing divergence and adaptation among member species that may lead to a more complete evolutionary conceptual model.

  5. Allantoinase in lake trout (Salvelinus namaycush): In vitro effects of PCBs, DDT and metals

    Science.gov (United States)

    Passino, Dora R. May; Cotant, Carol A.

    1979-01-01

    1. Allantoinase, an enzyme in the purine-urea cycle, was found in livers of Salvelinus namaycush (Osteichthyes: Salmoniformes).2. The enzyme was active from pH 6.6 to 8.2 at 37°C and from pH 7.4 to 9.0 at 10°C and had an Arrhenius energy was activation of 11.0 kcal/mol and a temperature quotient of 2.0. The Km of the enzyme homogenate was 8.4 mM allantoin.3. The concentrations of inorganic metals at which 50% inhibition occurred during in vitro exposure were 6.0 mg/l Cu2+, 6.7 mg/l Cd2+, 34 mg/l Hg2+ and 52 mg/l Pb2+. The in vitro sensitivity to PCBs, DDT and DDE and kinetics in the presence of metals were determined.4. Allantoinase activity was negatively correlated with body length for fish from Lake Michigan but not from Lake Superior or the laboratory.

  6. Spatial and temporal movement dynamics of brook Salvelinus fontinalis and brown trout Salmo trutta

    Science.gov (United States)

    Davis, L.A.; Wagner, Tyler; Barton, Meredith L.

    2015-01-01

    Native eastern brook trout Salvelinus fontinalis and naturalized brown trout Salmo trutta occur sympatrically in many streams across the brook trout’s native range in the eastern United States. Understanding within- among-species variability in movement, including correlates of movement, has implications for management and conservation. We radio tracked 55 brook trout and 45 brown trout in five streams in a north-central Pennsylvania, USA watershed to quantify the movement of brook trout and brown trout during the fall and early winter to (1) evaluate the late-summer, early winter movement patterns of brook trout and brown trout, (2) determine correlates of movement and if movement patterns varied between brook trout and brown trout, and (3) evaluate genetic diversity of brook trout within and among study streams, and relate findings to telemetry-based observations of movement. Average total movement was greater for brown trout (mean ± SD = 2,924 ± 4,187 m) than for brook trout (mean ± SD = 1,769 ± 2,194 m). Although there was a large amount of among-fish variability in the movement of both species, the majority of movement coincided with the onset of the spawning season, and a threshold effect was detected between stream flow and movement: where movement increased abruptly for both species during positive flow events. Microsatellite analysis of brook trout revealed consistent findings to those found using radio-tracking, indicating a moderate to high degree of gene flow among brook trout populations. Seasonal movement patterns and the potential for relatively large movements of brook and brown trout highlight the importance of considering stream connectivity when restoring and protecting fish populations and their habitats.

  7. The effects of inbreeding on sperm quality traits in captive‐bred lake trout, Salvelinus namaycush (Walbaum, 1972)

    DEFF Research Database (Denmark)

    Johnson, K.; Butts, I. A. E.; Smith, J. L.

    2015-01-01

    The effects of inbreeding in both captive and wild‐caught species and populations have been reported to affect a wide variety of life history traits. Recently, the effects of inbreeding on reproductive traits such as sperm quality have become a subject of particular interest for conservation...... biology, evolutionary ecology, and management of captive populations. This study investigated the effects of inbreeding on sperm quality in a captive population of experimentally inbred and outbred lake trout, Salvelinus namaycush. It was found for moderately to highly inbred males (males with half......‐sib and full‐sib parents, respectively), that sperm quality traits (velocity, motility, linearity, longevity, spermatocrit and morphology) showed no apparent inbreeding depression. The apparent lack of inbreeding effects on sperm quality traits may be due to several factors including (i) no inbreeding...

  8. Sexual difference in PCB concentrations of lake trout (Salvelinus namaycush) from Lake Ontario

    Science.gov (United States)

    Madenjian, Charles P.; Keir, Michael J.; Whittle, D. Michael; Noguchi, George E.

    2010-01-01

    We determined polychlorinated biphenyl (PCB) concentrations in 61 female lake trout (Salvelinus namaycush) and 71 male lake trout from Lake Ontario (Ontario, Canada and New York, United States). To estimate the expected change in PCB concentration due to spawning, PCB concentrations in gonads and in somatic tissue of lake trout were also determined. In addition, bioenergetics modeling was applied to investigate whether gross growth efficiency (GGE) differed between the sexes. Results showed that, on average, males were 22% higher in PCB concentration than females in Lake Ontario. Results from the PCB determinations of the gonads and somatic tissues revealed that shedding of the gametes led to 3% and 14% increases in PCB concentration for males and females, respectively. Therefore, shedding of the gametes could not explain the higher PCB concentration in male lake trout. According to the bioenergetics modeling results, GGE of males was about 2% higher than adult female GGE, on average. Thus, bioenergetics modeling could not explain the higher PCB concentrations exhibited by the males. Nevertheless, a sexual difference in GGE remained a plausible explanation for the sexual difference in PCB concentrations of the lake trout.

  9. Dynamics of individual growth in a recovering population of lake trout (Salvelinus namaycush)

    Science.gov (United States)

    Fabrizio, Mary C.; Dorazio, Robert M.; Schram, Stephen T.

    2001-01-01

    In 1976, the Wisconsin Department of Natural Resources established a refuge for a nearly depleted population of lake trout (Salvelinus namaycush) at Gull Island Shoal, Lake Superior. The refuge was intended to reduce fishing mortality by protecting adult lake trout. We examined the growth dynamics of these lake trout during the period of recovery by comparing estimates of ndividual growth before and after the refuge was established. Our estimates are based on an annual mark-recapture survey conducted at the spawning area since 1969. We developed a model that allowed mean growth rates to differ among individuals of different sizes and that accommodated variation in growth rates of individuals of the same size. Likelihood ratio tests were used to determine if the mean growth increments of lake trout changed ater the refuge was established. Our results suggest that growth of mature lake trout (particularly wild fish) decreased significantly in the postrefuge period. This decreased growth may have been associated with a reduction in food availability. We also observed reductions in growth as wild fish grew older and larger, which suggests that the growth of these fish may be adequately approximated by a von Bertalanffy growth model if it becomes possible to obtain accurate ages.

  10. Draft Genome Sequence of a Picorna-Like Virus Associated with Gill Tissue in Clinically Normal Brook Trout, Salvelinus fontinalis

    OpenAIRE

    Iwanowicz, Luke R.; Iwanowicz, Deborah D.; Adams, Cynthia R.; Galbraith, Heather; Aunins, Aaron; Cornman, Robert S.

    2017-01-01

    ABSTRACT Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.

  11. Draft Genome Sequence of a Picorna-Like Virus Associated with Gill Tissue in Clinically Normal Brook Trout, Salvelinus fontinalis.

    Science.gov (United States)

    Iwanowicz, Luke R; Iwanowicz, Deborah D; Adams, Cynthia R; Galbraith, Heather; Aunins, Aaron; Cornman, Robert S

    2017-10-12

    Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis , gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.

  12. Seasonal Variations in Relative Weight of Lake Trout (Salvelinus namaycush), Kokanee Salmon (Oncorhynchus nerka), Rainbow Trout (Onocorhynchus mykiss), and Brown Trout (Salmo trutta) in Blue Mesa Reservoir, Colorado

    OpenAIRE

    Midas, Madeline; Williams, Asia; Cooper, Cindy; Courtney, Michael

    2013-01-01

    Blue Mesa Reservoir is the largest body of water in Colorado and is located on the western slope of the Rocky Mountains at an elevation of 7520 feet. Blue Mesa Reservoir contains recreationally important populations of lake trout (Salvelinus namaycush), kokanee salmon (Oncorhynchus nerka), rainbow trout (Onocorhynchus mykiss), and brown trout (Salmo trutta). A management challenge in recent years has been the overpopulation of lake trout, which has led to a steep decline in abundance of kokan...

  13. Hypervariability of ribosomal DNA at multiple chromosomal sites in lake trout (Salvelinus namaycush).

    Science.gov (United States)

    Zhuo, L; Reed, K M; Phillips, R B

    1995-06-01

    Variation in the intergenic spacer (IGS) of the ribosomal DNA (rDNA) of lake trout (Salvelinus namaycush) was examined. Digestion of genomic DNA with restriction enzymes showed that almost every individual had a unique combination of length variants with most of this variation occurring within rather than between populations. Sequence analysis of a 2.3 kilobase (kb) EcoRI-DraI fragment spanning the 3' end of the 28S coding region and approximately 1.8 kb of the IGS revealed two blocks of repetitive DNA. Putative transcriptional termination sites were found approximately 220 bases (b) downstream from the end of the 28S coding region. Comparison of the 2.3-kb fragments with two longer (3.1 kb) fragments showed that the major difference in length resulted from variation in the number of short (89 b) repeats located 3' to the putative terminator. Repeat units within a single nucleolus organizer region (NOR) appeared relatively homogeneous and genetic analysis found variants to be stably inherited. A comparison of the number of spacer-length variants with the number of NORs found that the number of length variants per individual was always less than the number of NORs. Examination of spacer variants in five populations showed that populations with more NORs had more spacer variants, indicating that variants are present at different rDNA sites on nonhomologous chromosomes.

  14. Net trophic transfer efficiencies of polychlorinated biphenyl congeners to lake trout (Salvelinus namaycush) from its prey

    Science.gov (United States)

    Madenjian, Charles P.; David, Solomon R.; Rediske, Richard R.; O’Keefe, James P.

    2012-01-01

    Lake trout (Salvelinus namaycush) were fed bloater (Coregonus hoyi) in eight laboratory tanks over a 135-d experiment. At the start of the experiment, four to nine fish in each tank were sacrificed, and the concentrations of 75 polychlorinated biphenyl (PCB) congeners within these fish were determined. Polychlorinated biphenyl congener concentrations were also determined in the 10 lake trout remaining in each of the eight tanks at the end of the experiment as well as in the bloater fed to the lake trout. Each lake trout was weighed at the start and the end of the experiment, and the amount of food eaten by the lake trout was recorded. Using these measurements, net trophic transfer efficiency (γ) from the bloater to the lake trout in each of the eight tanks was calculated for each of the 75 congeners. Results showed that γ did not vary significantly with the degree of chlorination of the PCB congeners, and γ averaged 0.66 across all congeners. However,γ did show a slight, but significant, decrease as logKOW increased from 6.0 to 8.2. Activity level of the lake trout did not have a significant effect on γ.

  15. Chromosomal characteristics and distribution of rDNA sequences in the brook trout Salvelinus fontinalis (Mitchill, 1814).

    Science.gov (United States)

    Śliwińska-Jewsiewicka, A; Kuciński, M; Kirtiklis, L; Dobosz, S; Ocalewicz, K; Jankun, Malgorzata

    2015-08-01

    Brook trout Salvelinus fontinalis (Mitchill, 1814) chromosomes have been analyzed using conventional and molecular cytogenetic techniques enabling characteristics and chromosomal location of heterochromatin, nucleolus organizer regions (NORs), ribosomal RNA-encoding genes and telomeric DNA sequences. The C-banding and chromosome digestion with the restriction endonucleases demonstrated distribution and heterogeneity of the heterochromatin in the brook trout genome. DNA sequences of the ribosomal RNA genes, namely the nucleolus-forming 28S (major) and non-nucleolus-forming 5S (minor) rDNAs, were physically mapped using fluorescence in situ hybridization (FISH) and primed in situ labelling. The minor rDNA locus was located on the subtelo-acrocentric chromosome pair No. 9, whereas the major rDNA loci were dispersed on 14 chromosome pairs, showing a considerable inter-individual variation in the number and location. The major and minor rDNA loci were located at different chromosomes. Multichromosomal location (3-6 sites) of the NORs was demonstrated by silver nitrate (AgNO3) impregnation. All Ag-positive i.e. active NORs corresponded to the GC-rich blocks of heterochromatin. FISH with telomeric probe showed the presence of the interstitial telomeric site (ITS) adjacent to the NOR/28S rDNA site on the chromosome 11. This ITS was presumably remnant of the chromosome rearrangement(s) leading to the genomic redistribution of the rDNA sequences. Comparative analysis of the cytogenetic data among several related salmonid species confirmed huge variation in the number and the chromosomal location of rRNA gene clusters in the Salvelinus genome.

  16. Foraging mechanisms of siscowet lake trout (Salvelinus namaycush siscowet) on pelagic prey

    Science.gov (United States)

    Keyler, Trevor D.; Hrabik, Thomas R.; Austin, C. Lee; Gorman, Owen T.; Mensinger, Allen F.

    2015-01-01

    The reaction distance, angle of attack, and foraging success were determined for siscowet lake trout (Salvelinus namaycush siscowet) during laboratory trials under lighting conditions that approximated downwelling spectral irradiance and intensity (9.00 × 108–1.06 × 1014 photons m− 2 s− 1) at daytime depths. Siscowet reaction distance in response to golden shiners (Notemigonus crysoleucas) was directly correlated with increasing light intensity until saturation at 1.86 × 1011 photons m− 2 s− 1, above which reaction distance was constant within the range of tested light intensities. At the lowest tested light intensity, sensory detection was sufficient to locate prey at 25 ± 2 cm, while increasing light intensities increased reaction distance up to 59 ± 2 cm at 1.06 × 1014 photons m− 2 s− 1. Larger prey elicited higher reaction distances than smaller prey at all light intensities while moving prey elicited higher reaction distances than stationary prey at the higher light intensities (6.00 × 109 to 1.06 × 1014 photons m− 2 s− 1). The capture and consumption of prey similarly increased with increasing light intensity while time to capture decreased with increasing light intensity. The majority of orientations toward prey occurred within 120° of the longitudinal axis of the siscowet's eyes, although reaction distances among 30° increments along the entire axis were not significantly different. The developed predictive model will help determine reaction distances for siscowet in various photic environments and will help identify the mechanisms and behavior that allow for low light intensity foraging within freshwater systems.

  17. Feeding habits of the alien brook trout Salvelinus fontinalis and the native brown trout Salmo trutta in Czech mountain streams

    Directory of Open Access Journals (Sweden)

    Horká Petra

    2017-01-01

    Full Text Available Quantifying patterns of prey resource use is fundamental to identify mechanisms enabling the coexistence of related fish species. Trophic interactions between the native brown trout, Salmo trutta, and the introduced brook trout, Salvelinus fontinalis, were studied monthly from May to October in three mountain streams in Central Europe (Czech Republic. To evaluate whether the feeding habits differ between separated and coexisting populations of these species, one locality where both species coexist, and two allopatric populations of either species were studied. Across the study period, the mean stomach fullness of fish varied, being highest in spring and declining through autumn. The diet overlap (Schoener's overlap index between the species increased through the studied season (from 54.5% in July to 81.5% in October. In allopatry, both species had nearly the same feeding habits. However, in sympatry, brook trout consumed higher proportion of terrestrial invertebrates, while brown trout showed no changes either in the proportions of aquatic and terrestrial prey utilized or in the selectivity for prey categories in comparison to allopatric conditions. The dietary shift observed for brook trout, but not for brown trout, suggests that brown trout is a stronger competitor in the studied sympatric locality, leading the brook trout to change its feeding habits to reduce interspecific competition.

  18. Brook trout (Salvelinus fontinalis extinction in small boreal lakes revealed by ephippia pigmentation: a preliminary analysis

    Directory of Open Access Journals (Sweden)

    Alexandre Bérubé Tellier

    2016-12-01

    Full Text Available Ephippium pigmentation is a plastic trait which can be related to a trade-off between visual predation pressure and better protection of cladoceran eggs against different types of stress. Experimental studies showed that planktivorous fish exert a greater predation pressure on individuals carrying darker ephippia, but little is known about the variation of ephippium pigmentation along gradients of fish predation pressure in natural conditions. For this study, our experimental design included four small boreal lakes with known fish assemblages. Two of the lakes have viable brook trout (Salvelinus fontinalis populations, whereas the other two lakes experienced brook trout extinctions during the 20th century. Cladoceran ephippia were extracted from sediment cores at layers corresponding to the documented post- extinction phase (1990's and from an older layer (1950's for which the brook trout population status is not known precisely. Our first objective was to determine whether brook trout extinction has a direct effect on both ephippium pigmentation and size. Our second objective was to give a preliminary assessment of the status of brook trout populations in the 1950's by comparing the variation in ephippia traits measured from this layer to those measured in the 1990's, for which the extinction patterns are well known. Cost-effective image analysis was used to assess variation in pigmentation levels in ephippia. This approach provided a proxy for the amount of melanin invested in each ephippium analysed. Our study clearly shows that ephippium pigmentation may represent a better indicator of the presence of fish predators than ephippium size, a trait that showed a less clear pattern of variation between lakes with and without fish. For the 1990's period, ephippia from fishless lakes were darker and showed a slight tendency to be larger than ephippia from lakes with brook trout. However, no clear differences in either ephippium size or pigmentation

  19. Role of cortisol in stocking density-induced changes in growth and metabolism of brook charr (Salvelinus fontinalis)

    Energy Technology Data Exchange (ETDEWEB)

    Vijayan, M.M.

    1990-01-01

    Brook charr (Salvelinus fontinalis) held at high stocking density (SD) (120 kg/m{sup 3}) had a lower growth rate, food consumption and food conversion efficiency, and plasma thyroxine (T4) than those held at low SD (30 kg/m{sup 3}). SD had no effect on plasma triiodothyronine (T3) levels. Plasma cortisol levels in fish maintained at high SD were variable, being either lower with increased SD or not different from the low SD group. Head kidney tissue (containing the interrenal cells) preparations of brook charr held at high SD showed a higher spontaneous cortisol secretion rate. There was no difference in the clearance rate of ({sup 3}H)-cortisol from plasma, but liver from fish held at high SD showed higher cortisol uptake and catabolism, indicative of altered hepatic metabolic activity. High SD appears to alter the energy metabolism of brook charr. This was evident from significant changes between densities in levels of metabolites (plasma glucose and liver glycogen) and activities of key hepatic enzymes (PFK, HK, FBPase, G6PDH, HOAD, GK and G3PDH). These results suggests that high SD has the effect of mobilizing triglycerides, and promoting gluconeogenesis from glycerol, but has little effect on protein metabolism. When cortisol was administered to brook charr in the form of slow release intraperitoneal implants, the metabolic changes evident were similar to those observed in fish held at high SD. There was no consistent increase in plasma cortisol levels of cortisol implanted fish over a 90 day period. Nevertheless, there were significant effects, apparently cortisol-related, on certain metabolite levels (plasma glycerol, plasma glucose, hepatic glycogen), and activities of key hepatic enzymes.

  20. Breaking the speed limit--comparative sprinting performance of brook trout (Salvelinus fontinalis) and brown trout (Salmo trutta)

    Science.gov (United States)

    Castro-Santos, Theodore; Sanz-Ronda, Francisco Javier; Ruiz-Legazpi, Jorge

    2013-01-01

    Sprinting behavior of free-ranging fish has long been thought to exceed that of captive fish. Here we present data from wild-caught brook trout (Salvelinus fontinalis) and brown trout (Salmo trutta), volitionally entering and sprinting against high-velocity flows in an open-channel flume. Performance of the two species was nearly identical, with the species attaining absolute speeds > 25 body lengths·s−1. These speeds far exceed previously published observations for any salmonid species and contribute to the mounting evidence that commonly accepted estimates of swimming performance are low. Brook trout demonstrated two distinct modes in the relationship between swim speed and fatigue time, similar to the shift from prolonged to sprint mode described by other authors, but in this case occurring at speeds > 19 body lengths·s−1. This is the first demonstration of multiple modes of sprint swimming at such high swim speeds. Neither species optimized for distance maximization, however, indicating that physiological limits alone are poor predictors of swimming performance. By combining distributions of volitional swim speeds with endurance, we were able to account for >80% of the variation in distance traversed by both species.

  1. Preference and avoidance pH of brook trout Salvelinus fontinalis and brown trout Salmo trutta exposed to different holding pH.

    Science.gov (United States)

    Fost, B A; Ferreri, C P

    2015-08-01

    The goal of this study was to determine if short-term exposure of brook trout Salvelinus fontinalis and brown trout Salmo trutta to a lower pH than found in their source stream results in a shift in preference or avoidance pH. The lack of a shift in preference or avoidance pH of adult S. fontinalis and S. trutta suggests that these species can be held at a pH different from the source waterbody for a short period of time without altering preference or avoidance pH behaviour. © 2015 The Fisheries Society of the British Isles.

  2. Nucleotide variation in the mitochondrial genome provides evidence for dual routes of postglacial recolonization and genetic recombination in the northeastern brook trout (Salvelinus fontinalis).

    Science.gov (United States)

    Pilgrim, B L; Perry, R C; Barron, J L; Marshall, H D

    2012-09-26

    Levels and patterns of mitochondrial DNA (mtDNA) variation were examined to investigate the population structure and possible routes of postglacial recolonization of the world's northernmost native populations of brook trout (Salvelinus fontinalis), which are found in Labrador, Canada. We analyzed the sequence diversity of a 1960-bp portion of the mitochondrial genome (NADH dehydrogenase 1 gene and part of cytochrome oxidase 1) of 126 fish from 32 lakes distributed throughout seven regions of northeastern Canada. These populations were found to have low levels of mtDNA diversity, a characteristic trait of populations at northern extremes, with significant structuring at the level of the watershed. Upon comparison of northeastern brook trout sequences to the publicly available brook trout whole mitochondrial genome (GenBank AF154850), we infer that the GenBank sequence is from a fish whose mtDNA has recombined with that of Arctic charr (S. alpinus). The haplotype distribution provides evidence of two different postglacial founding groups contributing to present-day brook trout populations in the northernmost part of their range; the evolution of the majority of the haplotypes coincides with the timing of glacier retreat from Labrador. Our results exemplify the strong influence that historical processes such as glaciations have had on shaping the current genetic structure of northern species such as the brook trout.

  3. Sibship reconstruction for inferring mating systems, dispersal and effective population size in headwater brook trout (Salvelinus fontinalis) populations

    Science.gov (United States)

    Kanno, Yoichiro; Vokoun, Jason C.; Letcher, Benjamin H.

    2011-01-01

    Brook trout Salvelinus fontinalis populations have declined in much of the native range in eastern North America and populations are typically relegated to small headwater streams in Connecticut, USA. We used sibship reconstruction to infer mating systems, dispersal and effective population size of resident (non-anadromous) brook trout in two headwater stream channel networks in Connecticut. Brook trout were captured via backpack electrofishing using spatially continuous sampling in the two headwaters (channel network lengths of 4.4 and 7.7 km). Eight microsatellite loci were genotyped in a total of 740 individuals (80–140 mm) subsampled in a stratified random design from all 50 m-reaches in which trout were captured. Sibship reconstruction indicated that males and females were both mostly polygamous although single pair matings were also inferred. Breeder sex ratio was inferred to be nearly 1:1. Few large-sized fullsib families (>3 individuals) were inferred and the majority of individuals were inferred to have no fullsibs among those fish genotyped (family size = 1). The median stream channel distance between pairs of individuals belonging to the same large-sized fullsib families (>3 individuals) was 100 m (range: 0–1,850 m) and 250 m (range: 0–2,350 m) in the two study sites, indicating limited dispersal at least for the size class of individuals analyzed. Using a sibship assignment method, the effective population size for the two streams was estimated at 91 (95%CI: 67–123) and 210 (95%CI: 172–259), corresponding to the ratio of effective-to-census population size of 0.06 and 0.12, respectively. Both-sex polygamy, low variation in reproductive success, and a balanced sex ratio may help maintain genetic diversity of brook trout populations with small breeder sizes persisting in headwater channel networks.

  4. Fine-scale acoustic telemetry reveals unexpected lake trout, Salvelinus namaycush, spawning habitats in northern Lake Huron, North America

    Science.gov (United States)

    Binder, Thomas; Farha, Steve A.; Thompson, Henry T.; Holbrook, Christopher; Bergstedt, Roger A.; Riley, Stephen; Bronte, Charles R.; He, Ji; Krueger, Charles C.

    2018-01-01

    Previous studies of lake trout, Salvelinus namaycush, spawning habitat in the Laurentian Great Lakes have used time- and labour-intensive survey methods and have focused on areas with historic observations of spawning aggregations and on habitats prejudged by researchers to be suitable for spawning. As an alternative, we used fine-scale acoustic telemetry to locate, describe and compare lake trout spawning habitats. Adult lake trout were implanted with acoustic transmitters and tracked during five consecutive spawning seasons in a 19–27 km2 region of the Drummond Island Refuge, Lake Huron, using the VEMCO Positioning System. Acoustic telemetry revealed discrete areas of aggregation on at least five reefs in the study area, subsequently confirmed by divers to contain deposited eggs. Notably, several identified spawning sites would likely not have been discovered using traditional methods because either they were too small and obscure to stand out on a bathymetric map or because they did not conform to the conceptual model of spawning habitat held by many biologists. Our most unique observation was egg deposition in gravel and rubble substrates located at the base of and beneath overhanging edges of large boulders. Spawning sites typically comprised <10% of the reef area and were used consistently over the 5-year study. Evaluation of habitat selection from the perspective of fish behaviour through use of acoustic transmitters offers potential to expand current conceptual models of critical spawning habitat.

  5. Summer temperature metrics for predicting brook trout (Salvelinus fontinalis) distribution in streams

    Science.gov (United States)

    Parrish, Donna; Butryn, Ryan S.; Rizzo, Donna M.

    2012-01-01

    We developed a methodology to predict brook trout (Salvelinus fontinalis) distribution using summer temperature metrics as predictor variables. Our analysis used long-term fish and hourly water temperature data from the Dog River, Vermont (USA). Commonly used metrics (e.g., mean, maximum, maximum 7-day maximum) tend to smooth the data so information on temperature variation is lost. Therefore, we developed a new set of metrics (called event metrics) to capture temperature variation by describing the frequency, area, duration, and magnitude of events that exceeded a user-defined temperature threshold. We used 16, 18, 20, and 22°C. We built linear discriminant models and tested and compared the event metrics against the commonly used metrics. Correct classification of the observations was 66% with event metrics and 87% with commonly used metrics. However, combined event and commonly used metrics correctly classified 92%. Of the four individual temperature thresholds, it was difficult to assess which threshold had the “best” accuracy. The 16°C threshold had slightly fewer misclassifications; however, the 20°C threshold had the fewest extreme misclassifications. Our method leveraged the volumes of existing long-term data and provided a simple, systematic, and adaptable framework for monitoring changes in fish distribution, specifically in the case of irregular, extreme temperature events.

  6. Using Linkage Maps as a Tool To Determine Patterns of Chromosome Synteny in the Genus Salvelinus

    Directory of Open Access Journals (Sweden)

    Matthew C. Hale

    2017-11-01

    Full Text Available Next generation sequencing techniques have revolutionized the collection of genome and transcriptome data from nonmodel organisms. This manuscript details the application of restriction site-associated DNA sequencing (RADseq to generate a marker-dense genetic map for Brook Trout (Salvelinus fontinalis. The consensus map was constructed from three full-sib families totaling 176 F1 individuals. The map consisted of 42 linkage groups with a total female map size of 2502.5 cM, and a total male map size of 1863.8 cM. Synteny was confirmed with Atlantic Salmon for 38 linkage groups, with Rainbow Trout for 37 linkage groups, Arctic Char for 36 linkage groups, and with a previously published Brook Trout linkage map for 39 linkage groups. Comparative mapping confirmed the presence of 8 metacentric and 34 acrocentric chromosomes in Brook Trout. Six metacentric chromosomes seem to be conserved with Arctic Char suggesting there have been at least two species-specific fusion and fission events within the genus Salvelinus. In addition, the sex marker (sdY; sexually dimorphic on the Y chromosome was mapped to Brook Trout BC35, which is homologous with Atlantic Salmon Ssa09qa, Rainbow Trout Omy25, and Arctic Char AC04q. Ultimately, this linkage map will be a useful resource for studies on the genome organization of Salvelinus, and facilitates comparisons of the Salvelinus genome with Salmo and Oncorhynchus.

  7. New insight into the spawning behavior of lake trout, Salvelinus namaycush, from a recovering population in the Laurentian Great Lakes

    Science.gov (United States)

    Binder, Thomas R.; Thompson, Henry T.; Muir, Andrew M.; Riley, Stephen C.; Marsden, J. Ellen; Bronte, Charles R.; Krueger, Charles C.

    2015-01-01

    Spawning behavior of lake trout, Salvelinus namaycush, is poorly understood, relative to stream-dwelling salmonines. Underwater video records of spawning in a recovering population from the Drummond Island Refuge (Lake Huron) represent the first reported direct observations of lake trout spawning in the Laurentian Great Lakes. These observations provide new insight into lake trout spawning behavior and expand the current conceptual model. Lake trout spawning consisted of at least four distinct behaviors: hovering, traveling, sinking, and gamete release. Hovering is a new courtship behavior that has not been previously described. The apparent concentration of hovering near the margin of the spawning grounds suggests that courtship and mate selection might be isolated from the spawning act (i.e., traveling, sinking, and gamete release). Moreover, we interpret jockeying for position displayed by males during traveling as a unique form of male-male competition that likely evolved in concert with the switch from redd-building to itinerant spawning in lake trout. Unlike previous models, which suggested that intra-sexual competition and mate selection do not occur in lake trout, our model includes both and is therefore consistent with evolutionary theory, given that the sex ratio on spawning grounds is skewed heavily towards males. The model presented in this paper is intended as a working hypothesis, and further revision may become necessary as we gain a more complete understanding of lake trout spawning behavior.

  8. Exposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush)

    Science.gov (United States)

    Luoma, James A.; Severson, Todd J.; Wise, Jeremy K.; Barbour, Matthew

    2018-01-01

    The environmental fate, persistence, and non-target animal impacts of traditional molluscicides for zebra, Dreissena polymorpha, and quagga, D. bugensis, mussel control led to the development of the biomolluscicide Zequanox. Although previous research has demonstrated the specificity of Zequanox, one study indicated sensitivity of salmonids and lake sturgeon, Acipenser fulvescens, following non-label compliant exposures to Zequanox. This study was conducted to evaluate sublethal and lethal impacts of Zequanox exposure on juvenile lake sturgeon and lake trout, Salvelinus namaycush, following applications that were conducted in a manner consistent with the Zequanox product label. Fish were exposed to 50 or 100 mg/L of Zequanox as active ingredient for 8 h and then held for 33 d to evaluate latent impacts. No acute mortality was observed in either species; however, significant latent mortality (P < 0.01, df = 9; 46.2%) was observed in lake trout that were exposed to the highest dose of Zequanox. Statistically significant (P < 0.03, df = 9), but biologically minimal differences were observed in the weight (range 20.17 to 21.49 g) of surviving lake sturgeon at the termination of the 33 d post-exposure observation period. Statistically significant (P < 0.05, df = 9) and biologically considerable differences were observed in the weight (range 6.19 to 9.55 g) of surviving lake trout at the termination of the 33 d post-exposure observation period. Histologic evaluation of lake trout gastrointestinal tracts suggests that the mode of action in lake trout is different from the mode of action that induces zebra and quagga mussel mortality. Further research could determine the sensitivity of other salmonid species to Zequanox and determine if native fish will avoid Zequanox treated water.

  9. Evolution and origin of sympatric shallow-water morphotypes of Lake Trout, Salvelinus namaycush, in Canada's Great Bear Lake.

    Science.gov (United States)

    Harris, L N; Chavarie, L; Bajno, R; Howland, K L; Wiley, S H; Tonn, W M; Taylor, E B

    2015-01-01

    Range expansion in north-temperate fishes subsequent to the retreat of the Wisconsinan glaciers has resulted in the rapid colonization of previously unexploited, heterogeneous habitats and, in many situations, secondary contact among conspecific lineages that were once previously isolated. Such ecological opportunity coupled with reduced competition likely promoted morphological and genetic differentiation within and among post-glacial fish populations. Discrete morphological forms existing in sympatry, for example, have now been described in many species, yet few studies have directly assessed the association between morphological and genetic variation. Morphotypes of Lake Trout, Salvelinus namaycush, are found in several large-lake systems including Great Bear Lake (GBL), Northwest Territories, Canada, where several shallow-water forms are known. Here, we assess microsatellite and mitochondrial DNA variation among four morphotypes of Lake Trout from the five distinct arms of GBL, and also from locations outside of this system to evaluate several hypotheses concerning the evolution of morphological variation in this species. Our data indicate that morphotypes of Lake Trout from GBL are genetically differentiated from one another, yet the morphotypes are still genetically more similar to one another compared with populations from outside of this system. Furthermore, our data suggest that Lake Trout colonized GBL following dispersal from a single glacial refugium (the Mississippian) and support an intra-lake model of divergence. Overall, our study provides insights into the origins of morphological and genetic variation in post-glacial populations of fishes and provides benchmarks important for monitoring Lake Trout biodiversity in a region thought to be disproportionately susceptible to impacts from climate change.

  10. Use of the Rigor Mortis Process as a Tool for Better Understanding of Skeletal Muscle Physiology: Effect of the Ante-Mortem Stress on the Progression of Rigor Mortis in Brook Charr (Salvelinus fontinalis).

    Science.gov (United States)

    Diouf, Boucar; Rioux, Pierre

    1999-01-01

    Presents the rigor mortis process in brook charr (Salvelinus fontinalis) as a tool for better understanding skeletal muscle metabolism. Describes an activity that demonstrates how rigor mortis is related to the post-mortem decrease of muscular glycogen and ATP, how glycogen degradation produces lactic acid that lowers muscle pH, and how…

  11. Sex reversal of brook trout (Salvelinus fontinalis) by 17α-methyltestosterone exposure: A serial experimental approach to determine optimal timing and delivery regimes.

    Science.gov (United States)

    Fatima, Shafaq; Adams, Mark; Wilkinson, Ryan

    2016-12-01

    Commercial culture of Brook trout (Salvelinus fontinalis) in Tasmania was partly abandoned due to sexual maturation of male fish early on during the estuarine rearing phase. Maturation adversely affects body mass, flesh quality and immunocompetency effectively. Sex reversal techniques such as the in-feed addition of a synthetic androgen have proven difficult to adapt in brook trout. An appropriate timing, duration and delivery vehicle for administration of 17α-methyltestosterone (MT) to produce phenotypic males (neomales) from genotypically female brook trout required further investigation. In this study, groups of brook trout eggs (n=1000) maintained at 9.5±0.15-10±0.14°C, were immersed in MT (400μgL -1 ) for four hours on two alternate days (two immersions/group) staggered over a two week period surrounding the hatch of embryos (control groups excluded). The groups were then split and half received MT-supplemented feed for 60days and the other a standard diet. Following an 11 month on-growing period sex phenotypes were determined by gross & histological gonad morphology. The highest proportion of male phenotypes (75%) was found in fish immersed six and four days pre-hatch and subsequently fed a normal diet. Fish fed a MT supplemented diet and immersed in MT showed significantly higher proportions of sterile fish. These data indicate that a pre-hatch immersion-only regime (4-6days pre-hatch at 9.5°C) should be pursued as a target for optimization studies to further refine the effective concentration and duration of exposure to MT for the successful production of neo-male brook trout. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Competition and predation as mechanisms for displacement of greenback cutthroat trout by brook trout

    Science.gov (United States)

    C. C. McGrath; W. M. Lewis

    2007-01-01

    Cutthroat trout Oncorhynchus clarkii frequently are displaced by nonnative brook trout Salvelinus fontinalis, but the ecological mechanisms of displacement are not understood. Competition for food and predation between greenback cutthroat trout O. c. stomias and brook trout were investigated in montane streams of...

  13. Hiding in Plain Sight: A Case for Cryptic Metapopulations in Brook Trout (Salvelinus fontinalis.

    Directory of Open Access Journals (Sweden)

    David C Kazyak

    Full Text Available A fundamental issue in the management and conservation of biodiversity is how to define a population. Spatially contiguous fish occupying a stream network have often been considered to represent a single, homogenous population. However, they may also represent multiple discrete populations, a single population with genetic isolation-by-distance, or a metapopulation. We used microsatellite DNA and a large-scale mark-recapture study to assess population structure in a spatially contiguous sample of Brook Trout (Salvelinus fontinalis, a species of conservation concern. We found evidence for limited genetic exchange across small spatial scales and in the absence of barriers to physical movement. Mark-recapture and stationary passive integrated transponder antenna records demonstrated that fish from two tributaries very seldom moved into the opposite tributary, but movements between the tributaries and mainstem were more common. Using Bayesian genetic clustering, we identified two genetic groups that exhibited significantly different growth rates over three years of study, yet survival rates were very similar. Our study highlights the importance of considering the possibility of multiple genetically distinct populations occurring within spatially contiguous habitats, and suggests the existence of a cryptic metapopulation: a spatially continuous distribution of organisms exhibiting metapopulation-like behaviors.

  14. Global climate change and fragmentation of native brook trout distribution in the southern Appalachian Mountains

    Science.gov (United States)

    Patricia A. Flebbe

    1997-01-01

    Current distributions of native brook trout (Salvelinus fontinalis) in the Southern Appalachians are restricted to upper elevations by multiple factors, including habitat requirements, introduced rainbow (Oncorhynchus mykiss) and brown (Salmo trutta) trout, and other human activities. Present-day distribution of brook trout habitat is already fragmented. Increased...

  15. Brook trout movement during and after recolonization of a naturally defaunated stream reach

    Science.gov (United States)

    Craig N. Roghair; C. Andrew Dolloff

    2005-01-01

    In june 1995 a debris flow associated with a massive streamwide flood completely eliminated brook trout Salvelinus fontinalis from the lower 1.9 km of the Staunton River in Shenandoah National Park, Virginia. Biannual diver counts revealed that brook trout moved several hundred meters into the debris-flow-affected area each year, resulting in...

  16. Laboratory estimation of net trophic transfer efficiencies of PCB congeners to lake trout (Salvelinus namaycush) from its prey

    Science.gov (United States)

    Madenjian, Charles P.; Rediske, Richard R.; O'Keefe, James P.; David, Solomon R.

    2014-01-01

    A technique for laboratory estimation of net trophic transfer efficiency (γ) of polychlorinated biphenyl (PCB) congeners to piscivorous fish from their prey is described herein. During a 135-day laboratory experiment, we fed bloater (Coregonus hoyi) that had been caught in Lake Michigan to lake trout (Salvelinus namaycush) kept in eight laboratory tanks. Bloater is a natural prey for lake trout. In four of the tanks, a relatively high flow rate was used to ensure relatively high activity by the lake trout, whereas a low flow rate was used in the other four tanks, allowing for low lake trout activity. On a tank-by-tank basis, the amount of food eaten by the lake trout on each day of the experiment was recorded. Each lake trout was weighed at the start and end of the experiment. Four to nine lake trout from each of the eight tanks were sacrificed at the start of the experiment, and all 10 lake trout remaining in each of the tanks were euthanized at the end of the experiment. We determined concentrations of 75 PCB congeners in the lake trout at the start of the experiment, in the lake trout at the end of the experiment, and in bloaters fed to the lake trout during the experiment. Based on these measurements, γ was calculated for each of 75 PCB congeners in each of the eight tanks. Mean γ was calculated for each of the 75 PCB congeners for both active and inactive lake trout. Because the experiment was replicated in eight tanks, the standard error about mean γ could be estimated. Results from this type of experiment are useful in risk assessment models to predict future risk to humans and wildlife eating contaminated fish under various scenarios of environmental contamination.

  17. The diet of introduced brook trout (Salvelinus fontinalis; Mitchill, 1814 in an alpine area and a literature review on its feeding ecology

    Directory of Open Access Journals (Sweden)

    Rocco Tiberti

    2016-05-01

    Full Text Available Introduced fish are a major threat for high altitude aquatic habitats and Salvelinus fontinalis have been widely used throughout the Alps for stocking lakes and rivers. Understanding its feeding ecology is a basic, but essential tool for interpreting its impact. To assess which factors determine the diet of S. fontinalis we analyzed more than 500 stomachs from several introduced populations from the Gran Paradiso National Park (GPNP, Western Italian Alps and we measured the availability of several prey groups (zooplankton, aquatic invertebrates, terrestrial invertebrates. We complemented the study with a short, but exhaustive literature review on the S. fontinalis feeding ecology. In general the food composition reflected the availability of prey -confirming that S. fontinalis is an opportunistic predator- and was influenced by habitat type (stream vs lake, fish size, and seasonality. The obtained results were discussed in the light of the existing literature on the feeding ecology and ecological impact of S. fontinalis. Large benthonic insects account for a substantial part of  the diet of stream dwelling brook trout, while they are almost absent both in the diet and in the prey species pool of lake-dwelling brook trout, probably reflecting a stronger ecological impact in the lakes.

  18. Fine-scale population structure and riverscape genetics of brook trout (Salvelinus fontinalis) distributed continuously along headwater channel networks

    Science.gov (United States)

    Kanno, Yoichiro; Vokoun, Jason C.; Letcher, Benjamin H.

    2011-01-01

    Linear and heterogeneous habitat makes headwater stream networks an ideal ecosystem in which to test the influence of environmental factors on spatial genetic patterns of obligatory aquatic species. We investigated fine-scale population structure and influence of stream habitat on individual-level genetic differentiation in brook trout (Salvelinus fontinalis) by genotyping eight microsatellite loci in 740 individuals in two headwater channel networks (7.7 and 4.4 km) in Connecticut, USA. A weak but statistically significant isolation-by-distance pattern was common in both sites. In the field, many tagged individuals were recaptured in the same 50-m reaches within a single field season (summer to fall). One study site was characterized with a hierarchical population structure, where seasonal barriers (natural falls of 1.5–2.5 m in height during summer base-flow condition) greatly reduced gene flow and perceptible spatial patterns emerged because of the presence of tributaries, each with a group of genetically distinguishable individuals. Genetic differentiation increased when pairs of individuals were separated by high stream gradient (steep channel slope) or warm stream temperature in this site, although the evidence of their influence was equivocal. In a second site, evidence for genetic clusters was weak at best, but genetic differentiation between individuals was positively correlated with number of tributary confluences. We concluded that the population-level movement of brook trout was limited in the study headwater stream networks, resulting in the fine-scale population structure (genetic clusters and clines) even at distances of a few kilometres, and gene flow was mitigated by ‘riverscape’ variables, particularly by physical barriers, waterway distance (i.e. isolation-by-distance) and the presence of tributaries.

  19. Production and evaluation of YY-male Brook Trout to eradicate nonnative wild brook trout populations

    Science.gov (United States)

    Kennedy, Patrick; Schill, Daniel J.; Meyer, Kevin A.; Campbell, Matthew R.; Vu, Ninh V.; Hansen, Michael J.

    2017-01-01

    Nonnative Brook Trout Salvelinus fontinalis were introduced throughout western North America in the early 1900s, resulting in widespread self-sustaining populations that are difficult to eradicate and often threaten native salmonid populations. A novel approach for their eradication involves use of YY male (MYY) Brook Trout (created in the hatchery by feminizing XY males and crossing them with normal XY males). If MYY Brook Trout survive after stocking, and reproduce successfully with wild females, in theory this could eventually drive the sex ratio of the wild population to 100% males, at which point the population would not be able to reproduce and would be eradicated. This study represents the first successful development of a FYY and MYY salmonid broodstock, which was produced in four years at relatively low cost. Field trials demonstrated that stocked hatchery MYY Brook Trout survived and produced viable MYY offspring in streams, although reproductive fitness appeared to have been lower than their wild conspecifics. Even if reduced fitness is the norm in both streams and alpine lakes, our population simulations suggest that eradication can be achieved in reasonable time periods under some MYY stocking scenarios, especially when wild Brook Trout are simultaneously suppressed in the population.

  20. Fine Sediment Effects on Brook Trout Eggs in Laboratory Streams

    Science.gov (United States)

    David G. Argent; Patricia A. Flebbe

    1999-01-01

    This study was designed to determine effects of different fine sediments (0.43-0.85 mm in diameter) on survival of brook trout (Salvelinus fontinalis) eggs during early developmental stages under laboratory conditions. Intragravel permeability and dissolved oxygen declined with increasing fine sediment amounts. Survival at each developmental stage...

  1. Genetically based population divergence in overwintering energy mobilization in brook charr (Salvelinus fontinalis).

    Science.gov (United States)

    Crespel, Amélie; Bernatchez, Louis; Garant, Dany; Audet, Céline

    2013-03-01

    Investigating the nature of physiological traits potentially related to fitness is important towards a better understanding of how species and/or populations may respond to selective pressures imposed by contrasting environments. In northern species in particular, the ability to mobilize energy reserves to compensate for the low external energy intake during winter is crucial. However, the phenotypic and genetic bases of energy reserve accumulation and mobilization have rarely been investigated, especially pertaining to variation in strategy adopted by different populations. In the present study, we documented variation in several energy reserve variables and estimated their quantitative genetic basis to test the null hypothesis of no difference in variation at those traits among three strains of brook charr (Salvelinus fontinalis) and their reciprocal hybrids. Our results indicate that the strategy of winter energy preparation and mobilization was specific to each strain, whereby (1) domestic fish accumulated a higher amount of energy reserves before winter and kept accumulating liver glycogen during winter despite lower feeding; (2) Laval fish used liver glycogen and lipids during winter and experienced a significant decrease in condition factor; (3) Rupert fish had relatively little energy reserves accumulated at the end of fall and preferentially mobilized visceral fat during winter. Significant heritability for traits related to the accumulation and use of energy reserves was found in the domestic and Laval but not in the Rupert strain. Genetic and phenotypic correlations also varied among strains, which suggested population-specific genetic architecture underlying the expression of these traits. Hybrids showed limited evidence of non-additive effects. Overall, this study provides the first evidence of a genetically based-and likely adaptive-population-specific strategy for energy mobilization related to overwinter survival.

  2. Brook trout movement within a high-elevation watershed: Consequences for watershed restoration

    Science.gov (United States)

    Jeff L. Hansbarger; J. Todd Petty; Patricia M. Mazik

    2010-01-01

    We used radio-telemetry to quantify brook trout (Salvelinus fontinalis) and brown trout (Salmo trutta) movements in the Shavers Fork of the Cheat River, West Virginia, and an adjacent second-order tributary (Rocky Run). Our objectives were to quantify the overall rate of trout movement, assess spatial and temporal variation in...

  3. Sex Chromosome Evolution, Heterochiasmy, and Physiological QTL in the Salmonid Brook Charr Salvelinus fontinalis

    Directory of Open Access Journals (Sweden)

    Ben J.G. Sutherland

    2017-08-01

    Full Text Available Whole-genome duplication (WGD can have large impacts on genome evolution, and much remains unknown about these impacts. This includes the mechanisms of coping with a duplicated sex determination system and whether this has an impact on increasing the diversity of sex determination mechanisms. Other impacts include sexual conflict, where alleles having different optimums in each sex can result in sequestration of genes into nonrecombining sex chromosomes. Sex chromosome development itself may involve sex-specific recombination rate (i.e., heterochiasmy, which is also poorly understood. The family Salmonidae is a model system for these phenomena, having undergone autotetraploidization and subsequent rediploidization in most of the genome at the base of the lineage. The salmonid master sex determining gene is known, and many species have nonhomologous sex chromosomes, putatively due to transposition of this gene. In this study, we identify the sex chromosome of Brook Charr Salvelinus fontinalis and compare sex chromosome identities across the lineage (eight species and four genera. Although nonhomology is frequent, homologous sex chromosomes and other consistencies are present in distantly related species, indicating probable convergence on specific sex and neo-sex chromosomes. We also characterize strong heterochiasmy with 2.7-fold more crossovers in maternal than paternal haplotypes with paternal crossovers biased to chromosome ends. When considering only rediploidized chromosomes, the overall heterochiasmy trend remains, although with only 1.9-fold more recombination in the female than the male. Y chromosome crossovers are restricted to a single end of the chromosome, and this chromosome contains a large interspecific inversion, although its status between males and females remains unknown. Finally, we identify quantitative trait loci (QTL for 21 unique growth, reproductive, and stress-related phenotypes to improve knowledge of the genetic

  4. Behavioural and physiological responses of brook trout Salvelinus fontinalis to midwinter flow reduction in a small ice-free mountain stream.

    Science.gov (United States)

    Krimmer, A N; Paul, A J; Hontela, A; Rasmussen, J B

    2011-09-01

    This study presents an experimental analysis of the effects of midwinter flow reduction (50-75%, reduction in discharge in 4 h daily pulses) on the physical habitat and on behaviour and physiology of overwintering brook trout Salvelinus fontinalis in a small mountain stream. Flow reduction did not result in significant lowering of temperature or formation of surface or subsurface ice. The main findings were (1) daily movement by S. fontinalis increased (c. 2·5-fold) during flow reduction, but was limited to small-scale relocations (reduced during flow reduction. (3) Although both experimental and reference fish did lose mass and condition during the experiment, no effects of flow reduction on stress indicators (blood cortisol or glucose) or bioenergetics (total body fat, water content or mass loss) were detected, probably because access to the preferred type of cover remained available. Like other salmonids, S. fontinalis moves little and seeks physical cover during winter. Unlike many of the more studied salmonids, however, this species overwinters successfully in small groundwater-rich streams that often remain ice-free, and this study identifies undercut banks as the critical winter habitat rather than substratum cover. © 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

  5. Brook trout use of thermal refugia and foraging habitat influenced by brown trout

    Science.gov (United States)

    Hitt, Nathaniel P.; Snook, Erin; Massie, Danielle L.

    2017-01-01

    The distribution of native brook trout (Salvelinus fontinalis) in eastern North America is often limited by temperature and introduced brown trout (Salmo trutta), the relative importance of which is poorly understood but critical for conservation and restoration planning. We evaluated effects of brown trout on brook trout behavior and habitat use in experimental streams across increasing temperatures (14–23 °C) with simulated groundwater upwelling zones providing thermal refugia (6–9 °C below ambient temperatures). Allopatric and sympatric trout populations increased their use of upwelling zones as ambient temperatures increased, demonstrating the importance of groundwater as thermal refugia in warming streams. Allopatric brook trout showed greater movement rates and more even spatial distributions within streams than sympatric brook trout, suggesting interference competition by brown trout for access to forage habitats located outside thermal refugia. Our results indicate that removal of introduced brown trout may facilitate native brook trout expansion and population viability in downstream reaches depending in part on the spatial configuration of groundwater upwelling zones.

  6. Does the introduced brook trout ( Salvelinus fontinalis) affect growth of the native brown trout ( Salmo trutta)?

    Science.gov (United States)

    Korsu, Kai; Huusko, Ari; Muotka, Timo

    2009-03-01

    Non-native brook trout have become widely established in North European streams. We combined evidence from an artificial-stream experiment and drainage-scale field surveys to examine whether brook trout suppressed the growth of the native brown trout (age 0 to age 2). Our experimental results demonstrated that brown trout were unaffected by the presence of brook trout but that brook trout showed reduced growth in the presence of brown trout. However, the growth reduction only appeared in the experimental setting, indicating that the reduced spatial constraint of the experimental system may have forced the fish to unnaturally intense interactions. Indeed, in the field, no effect of either species on the growth of the putative competitor was detected. These results caution against uncritical acceptance of findings from small-scale experiments because they rarely scale up to more complex field situations. This and earlier work suggest that the establishment of brook trout in North European streams has taken place mainly because of the availability of unoccupied (or underutilized) niche space, rather than as a result of species trait combinations or interspecific competition per se.

  7. Perturbation in protein expression of the sterile salmonid hybrids between female brook trout Salvelinus fontinalis and male masu salmon Oncorhynchus masou during early spermatogenesis.

    Science.gov (United States)

    Zheng, Liang; Senda, Yoshie; Abe, Syuiti

    2013-05-01

    Most males and females of intergeneric hybrid (BM) between female brook trout (Bt) Salvelinus fontinalis and male masu salmon (Ms) Oncorhynchus masou had undeveloped gonads, with abnormal germ cell development shown by histological examination. To understand the cause of this hybrid sterility, expression profiles of testicular proteins in the BM and parental species were examined with 2-DE coupled with MALDI-TOF/TOF MS. Compared with the parental species, more than 60% of differentially expressed protein spots were down-regulated in BM. A total of 16 up-regulated and 48 down-regulated proteins were identified in BM. Up-regulated were transferrin and other somatic cell-predominant proteins, whereas down-regulated were some germ cell-specific proteins such as DEAD box RNA helicase Vasa. Other pronouncedly down-regulated proteins included tubulins and heat shock proteins that are supposed to have roles in spermatogenesis. The present findings suggest direct association of the observed perturbation in protein expression with the failure of spermatogenesis and the sterility in the examined salmonid hybrids. Copyright © 2013 Elsevier B.V. All rights reserved.

  8. Maintenance of phenotypic variation: repeatibility, heritability, and size-dependent processes in a wild brook trout population

    Science.gov (United States)

    Benjamin H. Letcher; Jason A Coombs; Keith H. Nislow

    2011-01-01

    Phenotypic variation in body size can result from within-cohort variation in birth dates, among-individual growth variation and size-selective processes. We explore the relative effects of these processes on the maintenance of wide observed body size variation in stream-dwelling brook trout (Salvelinus fontinalis). Based on the analyses of multiple...

  9. Ranking site vulnerability to increasing temperatures in southern Appalachian brook trout streams in Virginia: An exposure-sensitivity approach

    Science.gov (United States)

    Bradly A. Trumbo; Keith H. Nislow; Jonathan Stallings; Mark Hudy; Eric P. Smith; Dong-Yun Kim; Bruce Wiggins; Charles A. Dolloff

    2014-01-01

    Models based on simple air temperature–water temperature relationships have been useful in highlighting potential threats to coldwater-dependent species such as Brook Trout Salvelinus fontinalis by predicting major losses of habitat and substantial reductions in geographic distribution. However, spatial variability in the relationship between changes...

  10. Brook trout passage performance through culverts

    Science.gov (United States)

    Goerig, Elsa; Castro-Santos, Theodore R.; Bergeron, Normand

    2016-01-01

    Culverts can restrict access to habitat for stream-dwelling fishes. We used passive integrated transponder telemetry to quantify passage performance of >1000 wild brook trout (Salvelinus fontinalis) attempting to pass 13 culverts in Quebec under a range of hydraulic and environmental conditions. Several variables influenced passage success, including complex interactions between physiology and behavior, hydraulics, and structural characteristics. The probability of successful passage was greater through corrugated metal culverts than through smooth ones, particularly among smaller fish. Trout were also more likely to pass at warmer temperatures, but this effect diminished above 15 °C. Passage was impeded at higher flows, through culverts with steep slopes, and those with deep downstream pools. This study provides insight on factors influencing brook trout capacity to pass culverts as well as a model to estimate passage success under various conditions, with an improved resolution and accuracy over existing approaches. It also presents methods that could be used to investigate passage success of other species, with implications for connectivity of the riverscape.

  11. pH preference and avoidance responses of adult brook trout Salvelinus fontinalis and brown trout Salmo trutta.

    Science.gov (United States)

    Fost, B A; Ferreri, C P

    2015-03-01

    The pH preferred and avoided by wild, adult brook trout Salvelinus fontinalis and brown trout Salmo trutta was examined in a series a laboratory tests using gradual and steep-gradient flow-through aquaria. The results were compared with those published for the observed segregation patterns of juvenile S. fontinalis and S. trutta in Pennsylvania streams. The adult S. trutta tested showed a preference for pH 4·0 while adult S. fontinalis did not prefer any pH within the range tested. Salmo trutta are not found in Pennsylvania streams with a base-flow pH < 5·8 which suggests that S. trutta prefer pH well above 4·0. Adult S. trutta displayed a lack of avoidance at pH below 5·0, as also reported earlier for juveniles. The avoidance pH of wild, adult S. fontinalis (between pH 5·5 and 6·0) and S. trutta (between pH 6·5 and 7·0) did not differ appreciably from earlier study results for the avoidance pH of juvenile S. fontinalis and S. trutta. A comparison of c.i. around these avoidance estimates indicates that avoidance pH is similar among adult S. fontinalis and S. trutta in this study. The limited overlap of c.i. for avoidance pH values for the two species, however, suggests that some S. trutta will display avoidance at a higher pH when S. fontinalis will not. The results of this study indicate that segregation patterns of adult S. fontinalis and S. trutta in Pennsylvania streams could be related to pH and that competition with S. trutta could be mediating the occurrence of S. fontinalis at some pH levels. © 2015 The Fisheries Society of the British Isles.

  12. Invasion by non-native brook trout in Panther Creek, Idaho: Roles of habitat quality, biotic resistance, and connectivity to source habitats

    Science.gov (United States)

    Joseph R. Benjamin; Jason B. Dunham; Matthew R. Dare

    2007-01-01

    Theoretical models and empirical evidence suggest that the invasion of nonnative species in freshwaters is facilitated through the interaction of three factors: habitat quality, biotic resistance, and connectivity. We measured variables that represented each factor to determine which were associated with the occurrence of nonnative brook trout Salvelinus...

  13. Analysis of trade-offs between threats of invasion by nonnative brook trout (Salvelinus fontinalis) and intentional isolation for native westslope cutthroat trout (Oncorhynchus clarkii lewisi)

    Science.gov (United States)

    Peterson, D.P.; Rieman, B.E.; Dunham, J.B.; Fausch, K.D.; Young, M.K.

    2008-01-01

    Native salmonid fishes often face simultaneous threats from habitat fragmentation and invasion by nonnative trout species. Unfortunately, management actions to address one may create or exacerbate the other. A consistent decision process would include a systematic analysis of when and where intentional use or removal of barriers is the most appropriate action. We developed a Bayesian belief network as a tool for such analyses. We focused on native westslope cutthroat trout (Oncorhynchus clarkii lewisi) and nonnative brook trout (Salvelinus fontinalis) and considered the environmental factors influencing both species, their potential interactions, and the effects of isolation on the persistence of local cutthroat trout populations. The trade-offs between isolation and invasion were strongly influenced by size and habitat quality of the stream network to be isolated and existing demographic linkages within and among populations. An application of the model in several sites in western Montana (USA) showed the process could help clarify management objectives and options and prioritize conservation actions among streams. The approach can also facilitate communication among parties concerned with native salmonids, nonnative fish invasions, barriers and intentional isolation, and management of the associated habitats and populations. ?? 2008 NRC.

  14. Broad-scale patterns of Brook Trout responses to introduced Brown Trout in New York

    Science.gov (United States)

    McKenna, James E.; Slattery, Michael T.; Kean M. Clifford,

    2013-01-01

    Brook Trout Salvelinus fontinalis and Brown Trout Salmo trutta are valuable sport fish that coexist in many parts of the world due to stocking introductions. Causes for the decline of Brook Trout within their native range are not clear but include competition with Brown Trout, habitat alteration, and repetitive stocking practices. New York State contains a large portion of the Brook Trout's native range, where both species are maintained by stocking and other management actions. We used artificial neural network models, regression, principal components analysis, and simulation to evaluate the effects of Brown Trout, environmental conditions, and stocking on the distribution of Brook Trout in the center of their native range. We found evidence for the decline of Brook Trout in the presence of Brown Trout across many watersheds; 22% of sampled reaches where both species were expected to occur contained only Brown Trout. However, a model of the direct relationship between Brook Trout and Brown Trout abundance explained less than 1% of data variation. Ordination showed extensive overlap of Brook Trout and Brown Trout habitat conditions, with only small components of the hypervolume (multidimensional space) being distinctive. Subsequent analysis indicated higher abundances of Brook Trout in highly forested areas, while Brown Trout were more abundant in areas with relatively high proportions of agriculture. Simulation results indicated that direct interactions and habitat conditions were relatively minor factors compared with the effects of repeated stocking of Brown Trout into Brook Trout habitat. Intensive annual stocking of Brown Trout could eliminate resident Brook Trout in less than a decade. Ecological differences, harvest behavior, and other habitat changes can exacerbate Brook Trout losses. Custom stocking scenarios with Brown Trout introductions at relatively low proportions of resident Brook Trout populations may be able to sustain healthy populations of both

  15. Metallothionein in brook trout (salvelinus fontinalis) as a biological indicator of inorganic chemical contaminant stress

    International Nuclear Information System (INIS)

    Hamilton, S.J.

    1985-01-01

    A technique for quantifying metallothionein was evaluated with fish tissue. Adult brook trout were administered 3 mg 109 cadmium/kg body weight by intraperitoneal injection over a 5 day period to induce metallothionein concentrations in liver and kidney tissues. The method was modified so cadmium bound to unsaturated metallothionein could be measured. The method gave precise measurements and was used to evaluate the toxicological significant of metallothionein in two 30-day chronic toxicity studies of cadmium on brook trout. In particular, metallothionein was evaluated as a biological indicator of inorganic chemical stress in brook trout. Pathological effects in animals resulting from exposure to inorganic chemicals is thought to occur when metallothionein's sequestering ability is exceeded; a phenomenon explained by the spillover hypothesis. The presence of free cadmium in tissues of fish from all exposures suggests metallothionein was not saturated with cadmium perhaps because of competition for binding sites on metallothionein between cadmium and other inorganic chemicals such as copper and zinc. Based on results of the two toxicity studies, the spillover hypothesis should be redefined to a continuum of toxic responses to varying balances between the relative abundance of inorganic chemicals present and their respective binding affinities for metallothionein

  16. Evaluation of catch-and-release regulations on Brook Trout in Pennsylvania streams

    Science.gov (United States)

    Jason Detar,; Kristine, David; Wagner, Tyler; Greene, Tom

    2014-01-01

    In 2004, the Pennsylvania Fish and Boat Commission implemented catch-and-release (CR) regulations on headwater stream systems to determine if eliminating angler harvest would result in an increase in the number of adult (≥100 mm) or large (≥175 mm) Brook Trout Salvelinus fontinalis. Under the CR regulations, angling was permitted on a year-round basis, no Brook Trout could be harvested at any time, and there were no tackle restrictions. A before-after–control-impact design was used to evaluate the experimental regulations. Brook Trout populations were monitored in 16 treatment (CR regulations) and 7 control streams (statewide regulations) using backpack electrofishing gear periodically for up to 15 years (from 1990 to 2003 or 2004) before the implementation of the CR regulations and over a 7–8-year period (from 2004 or 2005 to 2011) after implementation. We used Poisson mixed models to evaluate whether electrofishing catch per effort (CPE; catch/100 m2) of adult (≥100 mm) or large (≥175 mm) Brook Trout increased in treatment streams as a result of implementing CR regulations. Brook Trout CPE varied among sites and among years, and there was no significant effect (increase or decrease) of CR regulations on the CPE of adult or large Brook Trout. Results of our evaluation suggest that CR regulations were not effective at improving the CPE of adult or large Brook Trout in Pennsylvania streams. Low angler use, high voluntary catch and release, and slow growth rates in infertile headwater streams are likely the primary reasons for the lack of response.

  17. Fall and winter survival of brook trout and brown trout in a north-central Pennsylvania watershed

    Science.gov (United States)

    Sweka, John A.; Davis, Lori A.; Wagner, Tyler

    2017-01-01

    Stream-dwelling salmonids that spawn in the fall generally experience their lowest survival during the fall and winter due to behavioral changes associated with spawning and energetic deficiencies during this time of year. We used data from Brook Trout Salvelinus fontinalis and Brown Trout Salmo trutta implanted with radio transmitters in tributaries of the Hunts Run watershed of north-central Pennsylvania to estimate survival from the fall into the winter seasons (September 2012–February 2013). We examined the effects that individual-level covariates (trout species, size, and movement rates) and stream-level covariates (individual stream and cumulative drainage area of a stream) have on survival. Brook Trout experienced significantly lower survival than Brown Trout, especially in the early fall during their peak spawning period. Besides a significant species effect, none of the other covariates examined influenced survival for either species. A difference in life history between these species, with Brook Trout having a shorter life expectancy than Brown Trout, is likely the primary reason for the lower survival of Brook Trout. However, Brook Trout also spawn earlier in the fall than Brown Trout and low flows during Brook Trout spawning may have resulted in a greater risk of predation for Brook Trout compared with Brown Trout, thereby also contributing to the observed differences in survival between these species. Our estimates of survival can aid parameterization of future population models for Brook Trout and Brown Trout through the spawning season and into winter.

  18. Growth rate differences between resident native brook trout and non-native brown trout

    Science.gov (United States)

    Carlson, S.M.; Hendry, A.P.; Letcher, B.H.

    2007-01-01

    Between species and across season variation in growth was examined by tagging and recapturing individual brook trout Salvelinus fontinalis and brown trout Salmo trutta across seasons in a small stream (West Brook, Massachusetts, U.S.A.). Detailed information on body size and growth are presented to (1) test whether the two species differed in growth within seasons and (2) characterize the seasonal growth patterns for two age classes of each species. Growth differed between species in nearly half of the season- and age-specific comparisons. When growth differed, non-native brown trout grew faster than native brook trout in all but one comparison. Moreover, species differences were most pronounced when overall growth was high during the spring and early summer. These growth differences resulted in size asymmetries that were sustained over the duration of the study. A literature survey also indicated that non-native salmonids typically grow faster than native salmonids when the two occur in sympatry. Taken together, these results suggest that differences in growth are not uncommon for coexisting native and non-native salmonids. ?? 2007 The Authors.

  19. Evaluation of an Unsuccessful Brook Trout Electrofishing Removal Project in a Small Rocky Mountain Stream.

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Kevin A.; Lamansky, Jr., James A.; Schill, Daniel J.

    2006-01-26

    In the western United States, exotic brook trout Salvelinus fontinalis frequently have a deleterious effect on native salmonids, and biologists often attempt to remove brook trout from streams by means of electrofishing. Although the success of such projects typically is low, few studies have assessed the underlying mechanisms of failure, especially in terms of compensatory responses. A multiagency watershed advisory group (WAG) conducted a 3-year removal project to reduce brook trout and enhance native salmonids in 7.8 km of a southwestern Idaho stream. We evaluated the costs and success of their project in suppressing brook trout and looked for brook trout compensatory responses, such as decreased natural mortality, increased growth, increased fecundity at length, and earlier maturation. The total number of brook trout removed was 1,401 in 1998, 1,241 in 1999, and 890 in 2000; removal constituted an estimated 88% of the total number of brook trout in the stream in 1999 and 79% in 2000. Although abundance of age-1 and older brook trout declined slightly during and after the removals, abundance of age-0 brook trout increased 789% in the entire stream 2 years after the removals ceased. Total annual survival rate for age-2 and older brook trout did not decrease during the removals, and the removals failed to produce an increase in the abundance of native redband trout Oncorhynchus mykiss gairdneri. Lack of a meaningful decline and unchanged total mortality for older brook trout during the removals suggest that a compensatory response occurred in the brook trout population via reduced natural mortality, which offset the removal of large numbers of brook trout. Although we applaud WAG personnel for their goal of enhancing native salmonids by suppressing brook trout via electrofishing removal, we conclude that their efforts were unsuccessful and suggest that similar future projects elsewhere over such large stream lengths would be costly, quixotic enterprises.

  20. Is motivation important to brook trout passage through culverts?

    Science.gov (United States)

    Goerig, Elsa; Castro-Santos, Theodore R.

    2017-01-01

    Culverts can restrict movement of stream-dwelling fish. Motivation to enter and ascend these structures is an essential precursor for successful passage. However, motivation is challenging to quantify. Here, we use attempt rate to assess motivation of 447 brook trout (Salvelinus fontinalis) entering three culverts under a range of hydraulic, environmental, and biological conditions. A passive integrated transponder system allowed for the identification of passage attempts and success of individual fish. Attempt rate was quantified using time-to-event analysis allowing for time-varying covariates and recurrent events. Attempt rate was greatest during the spawning period, at elevated discharge, at dusk, and for longer fish. It decreased during the day and with increasing number of conspecifics downstream of the culvert. Results also show a positive correlation between elevated motivation and successful passage. This study enhances understanding of factors influencing brook trout motivation to ascend culverts and shows that attempt rate is a dynamic phenomenon, variable over time and among individuals. It also presents methods that could be used to investigate other species’ motivation to pass natural or anthropogenic barriers.

  1. Shale Gas Development and Brook Trout: Scaling Best Management Practices to Anticipate Cumulative Effects

    Science.gov (United States)

    Smith, David; Snyder, Craig D.; Hitt, Nathaniel P.; Young, John A.; Faulkner, Stephen P.

    2012-01-01

    Shale gas development may involve trade-offs between energy development and benefits provided by natural ecosystems. However, current best management practices (BMPs) focus on mitigating localized ecological degradation. We review evidence for cumulative effects of natural gas development on brook trout (Salvelinus fontinalis) and conclude that BMPs should account for potential watershed-scale effects in addition to localized influences. The challenge is to develop BMPs in the face of uncertainty in the predicted response of brook trout to landscape-scale disturbance caused by gas extraction. We propose a decision-analysis approach to formulating BMPs in the specific case of relatively undisturbed watersheds where there is consensus to maintain brook trout populations during gas development. The decision analysis was informed by existing empirical models that describe brook trout occupancy responses to landscape disturbance and set bounds on the uncertainty in the predicted responses to shale gas development. The decision analysis showed that a high efficiency of gas development (e.g., 1 well pad per square mile and 7 acres per pad) was critical to achieving a win-win solution characterized by maintaining brook trout and maximizing extraction of available gas. This finding was invariant to uncertainty in predicted response of brook trout to watershed-level disturbance. However, as the efficiency of gas development decreased, the optimal BMP depended on the predicted response, and there was considerable potential value in discriminating among predictive models through adaptive management or research. The proposed decision-analysis framework provides an opportunity to anticipate the cumulative effects of shale gas development, account for uncertainty, and inform management decisions at the appropriate spatial scales.

  2. Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout.

    Science.gov (United States)

    Glenney, Gavin W; Barbash, Patricia A; Coll, John A

    2016-03-01

    A novel herpesvirus was found by molecular methods in samples of Lake Trout Salvelinus namaycush from Lake Erie, Pennsylvania, and Lake Ontario, Keuka Lake, and Lake Otsego, New York. Based on PCR amplification and partial sequencing of polymerase, terminase, and glycoprotein genes, a number of isolates were identified as a novel virus, which we have named Namaycush herpesvirus (NamHV) salmonid herpesvirus 5 (SalHV5). Phylogenetic analyses of three NamHV genes indicated strong clustering with other members of the genus Salmonivirus, placing these isolates into family Alloherpesviridae. The NamHV isolates were identical in the three partially sequenced genes; however, they varied from other salmonid herpesviruses in nucleotide sequence identity. In all three of the genes sequenced, NamHV shared the highest sequence identity with Atlantic Salmon papillomatosis virus (ASPV; SalHV4) isolated from Atlantic Salmon Salmo salar in northern Europe, including northwestern Russia. These results lead one to believe that NamHV and ASPV have a common ancestor that may have made a relatively recent host jump from Atlantic Salmon to Lake Trout or vice versa. Partial nucleotide sequence comparisons between NamHV and ASPV for the polymerase and glycoprotein genes differ by >5% and >10%, respectively. Additional nucleotide sequence comparisons between NamHV and epizootic epitheliotropic disease virus (EEDV/SalHV3) in the terminase, glycoprotein, and polymerase genes differ by >5%, >20%, and >10%, respectively. Thus, NamHV and EEDV may be occupying discrete ecological niches in Lake Trout. Even though NamHV shared the highest genetic identity with ASPV, each of these viruses has a separate host species, which also implies speciation. Additionally, NamHV has been detected over the last 4 years in four separate water bodies across two states, which suggests that NamHV is a distinct, naturally replicating lineage. This, in combination with a divergence in nucleotide sequence from EEDV

  3. Differences in detection of Aeromonas salmonicida in covertly infected salmonid fishes by the stress-inducible furunculosis test and culture-based assays

    Science.gov (United States)

    Cipriano, R.C.; Ford, L.A.; Smith, D.R.; Schachte, J.H.; Petrie, C.J.

    1997-01-01

    Accurate detection of Aeromonas salmonicida subsp. salmonicida (the cause of furunculosis disease) in covertly infected salmonids is difficult and is a cause of concern for those involved in fish health inspection and resource management programs. In this study, we examined populations of brook trout Salvelinus fontinalis, Atlantic salmon Salmo salar, and lake trout Salvelinus namaycush that previously sustained natural episodes of furunculosis. Consequently, the sampled fish were presumed to harbor latent infections. Mucus, gill, liver, kidney, heart, spleen, and intestine samples (N = 100 fish per group sampled) were processed and examined by (1) direct dilution counts and (2) quadrant streaking after a 48-h pre-enrichment in trypticase soy broth (TSB). Another subsample of fish from each group was then subjected to stress-inducible furunculosis tests. Stress tests detected A. salmonicida in three of four groups of fish that were examined whereas the pathogen was detected in only two of the groups analyzed with culture-based assays. Although pre-enrichment in TSB enhanced detection within internal sampling sites including the liver, heart, spleen, and kidney, enrichment did not enhance detection from mucus, gill, or intestinal samples.

  4. The effects of varied densities on the growth and emigration of adult cutthroat trout and brook trout in fenced stream enclosures

    Science.gov (United States)

    Buys, D.J.; Hilderbrand, R.H.; Kershner, J.L.

    2009-01-01

    We evaluated the effects of various density treatments on adult fish growth and emigration rates between Bonneville cutthroat trout Oncorhynchus clarki utah and brook trout Salvelinus fontinalis in stream enclosures in Beaver Creek, Idaho, We used 3 density treatments (low, ambient, and high fish densities) to evaluate density-related effects and to ensure a response. Intraspecific ambient-density tests using cutthroat trout only were also performed. Results indicated an absence of cage effects in the stream enclosures and no differences in fish growth between ambient-density stream-enclosure fish and free-range fish. Brook trout outgrew and moved less than cutthroat trout in the stream enclosures, especially as density increased, In all 3 density treatments, brook trout gained more weight than cutthroat trout, with brook trout gaining weight in each density treatment and cutthroat trout losing weight at the highest density. At high densities, cutthroat trout attempted to emigrate more frequently than brook trout in sympatry and allopatry. We observed a negative correlation between growth and emigration for interspecific cutthroat trout, indicating a possible competitive response due to the presence of brook trout. We observed similar responses for weight and emigration in trials of allopatric cutthroat trout, indicating strong intraspecific effects as density increased. While cutthroat trout showed a response to experimental manipulation with brook trout at different densities, there has been long-term coexistence between these species in Beaver Creek, This system presents a unique opportunity to study the mechanisms that lead cutthroat trout to coexist with rather than be replaced by nonnative brook trout.

  5. Genetic and phenotypic variation along an ecological gradient in lake trout Salvelinus namaycush

    Science.gov (United States)

    Baillie, Shauna M.; Muir, Andrew M.; Hansen, Michael J.; Krueger, Charles C.; Bentzen, Paul

    2016-01-01

    BackgroundAdaptive radiation involving a colonizing phenotype that rapidly evolves into at least one other ecological variant, or ecotype, has been observed in a variety of freshwater fishes in post-glacial environments. However, few studies consider how phenotypic traits vary with regard to neutral genetic partitioning along ecological gradients. Here, we present the first detailed investigation of lake trout Salvelinus namaycushthat considers variation as a cline rather than discriminatory among ecotypes. Genetic and phenotypic traits organized along common ecological gradients of water depth and geographic distance provide important insights into diversification processes in a lake with high levels of human disturbance from over-fishing.ResultsFour putative lake trout ecotypes could not be distinguished using population genetic methods, despite morphological differences. Neutral genetic partitioning in lake trout was stronger along a gradient of water depth, than by locality or ecotype. Contemporary genetic migration patterns were consistent with isolation-by-depth. Historical gene flow patterns indicated colonization from shallow to deep water. Comparison of phenotypic (Pst) and neutral genetic variation (Fst) revealed that morphological traits related to swimming performance (e.g., buoyancy, pelvic fin length) departed more strongly from neutral expectations along a depth gradient than craniofacial feeding traits. Elevated phenotypic variance with increasing water depth in pelvic fin length indicated possible ongoing character release and diversification. Finally, differences in early growth rate and asymptotic fish length across depth strata may be associated with limiting factors attributable to cold deep-water environments.ConclusionWe provide evidence of reductions in gene flow and divergent natural selection associated with water depth in Lake Superior. Such information is relevant for documenting intraspecific biodiversity in the largest freshwater lake

  6. Haematological and physiological responses of brook charr, to untreated and limestone-neutralized acid mine drainage

    Energy Technology Data Exchange (ETDEWEB)

    Cole, M.B.; Arnold, D.E.; Watten, B.J.; Krise, W.F. [ABR Inc, Forest Grove, OR (USA). Environmental Research and Services

    2001-07-01

    Brook charr, Salvelinus fontinalis, exhibited depressed plasma sodium and elevated plasma glucose concentrations in untreated acid mine drainage effluent (AMD), at two dilutions. Plasma sodium and glucose concentrations remained stable in treated AMD, pulsed, fluidized beds of limestone and carbon-dioxide pre-treatment of influent, and in AMD-free water. Results indicate that effluents produced by this treatment system were not toxic to these fish, despite still containing moderate concentrations of manganese (3-4 mg l{sup 1} following dilution in exposure systems), and provide justification for field deployment and further biological testing of this treatment in the field.

  7. Exotic "Gill Lice" Species (Copepoda: Lernaeopodidae: Salmincola SPP.) Infect Rainbow Trout (Oncorhynchus mykiss) and Brook Trout (Salvelinus fontinalis) in the Southeastern United States.

    Science.gov (United States)

    Ruiz, Carlos F; Rash, Jacob M; Besler, Doug A; Roberts, Jackson R; Warren, Micah B; Arias, Cova R; Bullard, Stephen A

    2017-08-01

    Salmincola californiensis infected 25 of 31 (prevalence 0.8; intensity 2-35 [mean 6.6 ± standard deviation 7.7; n = 25]) rainbow trout, Oncorhynchus mykiss, from a private trout farm connected to the Watauga River, North Carolina. Salmincola edwardsii infected all of 9 (1.0; 2-43 [9.3 ± 13.0; 9]) brook trout, Salvelinus fontinalis, from Big Norton Prong, a tributary of the Little Tennessee River, North Carolina. Both lernaeopodids are well-known salmonid pathogens, but neither is native to, nor has been previously taxonomically confirmed from, the southeastern United States. Herein, we (1) use light and scanning electron microscopy to identify and provide supplemental morphological observations of these lernaeopodids, (2) furnish complementary molecular sequence data from the 28S rDNA (28S), and (3) document the pathological effects of gill infections. We identified and differentiated these lernaeopodids by the second antenna (exopod tip with large [S. californiensis] vs. slender [S. edwardsii] spines; endopod terminal segment with subequal ventral processes shorter than [S. californiensis] vs. longer than or equal to [S. edwardsii] dorsal hook), maxilliped palp (length typically ≤1/3 [S. californiensis] vs. 1/3-1/2 [S. edwardsii] subchela length exclusive of claw), and bulla (sub-circular and concave on manubrium's side [S. californiensis] vs. non-stellate [S. edwardsii]). Analysis of the 28S rDNA sequences confirmed our taxonomic assignments as demonstrated by 100% sequence similarity among the sympatric, morphologically-conspecific isolates. Histopathology revealed focal gill epithelial hyperplasia, obstruction of interlamellar water channels, lamellar fusion, and crypting of gill filaments. High intensity infections by either lernaeopodid are surveillance-worthy because they are potentially pathogenic to trout in the southeastern United States.

  8. Do native brown trout and non-native brook trout interact reproductively?

    Science.gov (United States)

    Cucherousset, J.; Aymes, J. C.; Poulet, N.; Santoul, F.; Céréghino, R.

    2008-07-01

    Reproductive interactions between native and non-native species of fish have received little attention compared to other types of interactions such as predation or competition for food and habitat. We studied the reproductive interactions between non-native brook trout ( Salvelinus fontinalis) and native brown trout ( Salmo trutta) in a Pyrenees Mountain stream (SW France). We found evidence of significant interspecific interactions owing to consistent spatial and temporal overlap in redd localizations and spawning periods. We observed mixed spawning groups composed of the two species, interspecific subordinate males, and presence of natural hybrids (tiger trout). These reproductive interactions could be detrimental to the reproduction success of both species. Our study shows that non-native species might have detrimental effects on native species via subtle hybridization behavior.

  9. Scale-dependent seasonal pool habitat use by sympatric Wild Brook Trout and Brown Trout populations

    Science.gov (United States)

    Davis, Lori A.; Wagner, Tyler

    2016-01-01

    Sympatric populations of native Brook Trout Salvelinus fontinalis and naturalized Brown Trout Salmo truttaexist throughout the eastern USA. An understanding of habitat use by sympatric populations is of importance for fisheries management agencies because of the close association between habitat and population dynamics. Moreover, habitat use by stream-dwelling salmonids may be further complicated by several factors, including the potential for fish to display scale-dependent habitat use. Discrete-choice models were used to (1) evaluate fall and early winter daytime habitat use by sympatric Brook Trout and Brown Trout populations based on available residual pool habitat within a stream network and (2) assess the sensitivity of inferred habitat use to changes in the spatial scale of the assumed available habitat. Trout exhibited an overall preference for pool habitats over nonpool habitats; however, the use of pools was nonlinear over time. Brook Trout displayed a greater preference for deep residual pool habitats than for shallow pool and nonpool habitats, whereas Brown Trout selected for all pool habitat categories similarly. Habitat use by both species was found to be scale dependent. At the smallest spatial scale (50 m), habitat use was primarily related to the time of year and fish weight. However, at larger spatial scales (250 and 450 m), habitat use varied over time according to the study stream in which a fish was located. Scale-dependent relationships in seasonal habitat use by Brook Trout and Brown Trout highlight the importance of considering scale when attempting to make inferences about habitat use; fisheries managers may want to consider identifying the appropriate spatial scale when devising actions to restore and protect Brook Trout populations and their habitats.

  10. A comparative and experimental evaluation of performance of stocked diploid and triploid brook trout

    Science.gov (United States)

    Budy, Phaedra E.; Thiede, G.P.; Dean, A.; Olsen, D.; Rowley, G.

    2012-01-01

    Despite numerous negative impacts, nonnative trout are still being stocked to provide economically and socially valuable sport fisheries in western mountain lakes. We evaluated relative performance and potential differences in feeding strategy and competitive ability of triploid versus diploid brook trout Salvelinus fontinalis in alpine lakes, as well as behavioral and performance differences of diploid and triploid brook trout in two controlled experimental settings: behavioral experiments in the laboratory and performance evaluations in ponds. Across lakes, catch per unit effort (CPUE) and relative weight (Wr ) were not significantly different between ploidy levels. Mean sizes were also similar between ploidy levels except in two of the larger lakes where diploids attained slightly larger sizes (approximately 20 mm longer). We observed no significant differences between diploids and triploids in diet, diet preference, or trophic structure. Similarly, growth and condition did not differ between ploidy levels in smaller-scale pond experiments, and aggressive behavior did not differ between ploidy levels (fed or unfed fish trials) in the laboratory. Independent of ploidy level, the relative performance of brook trout varied widely among lakes, a pattern that appeared to be a function of lake size or a factor that covaries with lake size such as temperature regime or carrying capacity. In summary, we observed no significant differences in the relative performance of brook trout from either ploidy level across a number of indices, systems, and environmental conditions, nor any indication that one group is more aggressive or a superior competitor than the other. Collectively, these results suggest that triploid brook trout will offer a more risk-averse and promising management opportunity when they are stocked to these lakes and elsewhere to simultaneously meet the needs for the sport fishery and conservation objectives.

  11. Spatial structure of morphological and neutral genetic variation in Brook Trout

    Science.gov (United States)

    Kazyak, David C.; Hilderbrand, Robert H.; Keller, Stephen R.; Colaw, Mark C.; Holloway, Amanda E.; Morgan, Raymond P.; King, Timothy L.

    2015-01-01

    Brook Trout Salvelinus fontinalis exhibit exceptional levels of life history variation, remarkable genetic variability, and fine-scale population structure. In many cases, neighboring populations may be highly differentiated from one another to an extent that is comparable with species-level distinctions in other taxa. Although genetic samples have been collected from hundreds of populations and tens of thousands of individuals, little is known about whether differentiation at neutral markers reflects phenotypic differences among Brook Trout populations. We compared differentiation in morphology and neutral molecular markers among populations from four geographically proximate locations (all within 24 km) to examine how genetic diversity covaries with morphology. We found significant differences among and/or within streams for all three morphological axes examined and identified the source stream of many individuals based on morphology (52.3% classification efficiency). Although molecular and morphological differentiation among streams ranged considerably (mean pairwise FST: 0.023–0.264; pairwise PST: 0.000–0.339), the two measures were not significantly correlated. While in some cases morphological characters appear to have diverged to a greater extent than expected by neutral genetic drift, many traits were conserved to a greater extent than were neutral genetic markers. Thus, while Brook Trout exhibit fine-scale spatial patterns in both morphology and neutral genetic diversity, these types of biological variabilities are being structured by different ecological and evolutionary processes. The relative influences of genetic drift versus selection and phenotypic plasticity in shaping morphology appear to vary among populations occupying nearby streams.

  12. Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis

    Science.gov (United States)

    Wilcox, Taylor M; Mckelvey, Kevin S.; Young, Michael K.; Sepulveda, Adam; Shepard, Bradley B.; Jane, Stephen F; Whiteley, Andrew R.; Lowe, Winsor H.; Schwartz, Michael K.

    2016-01-01

    Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection > 0.99 at densities of ≥ 3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods.

  13. Landscape-scale evaluation of asymmetric interactions between Brown Trout and Brook Trout using two-species occupancy models

    Science.gov (United States)

    Wagner, Tyler; Jefferson T. Deweber,; Jason Detar,; John A. Sweka,

    2013-01-01

    Predicting the distribution of native stream fishes is fundamental to the management and conservation of many species. Modeling species distributions often consists of quantifying relationships between species occurrence and abundance data at known locations with environmental data at those locations. However, it is well documented that native stream fish distributions can be altered as a result of asymmetric interactions between dominant exotic and subordinate native species. For example, the naturalized exotic Brown Trout Salmo trutta has been identified as a threat to native Brook Trout Salvelinus fontinalis in the eastern United States. To evaluate large-scale patterns of co-occurrence and to quantify the potential effects of Brown Trout presence on Brook Trout occupancy, we used data from 624 stream sites to fit two-species occupancy models. These models assumed that asymmetric interactions occurred between the two species. In addition, we examined natural and anthropogenic landscape characteristics we hypothesized would be important predictors of occurrence of both species. Estimated occupancy for Brook Trout, from a co-occurrence model with no landscape covariates, at sites with Brown Trout present was substantially lower than sites where Brown Trout were absent. We also observed opposing patterns for Brook and Brown Trout occurrence in relation to percentage forest, impervious surface, and agriculture within the network catchment. Our results are consistent with other studies and suggest that alterations to the landscape, and specifically the transition from a forested catchment to one that contains impervious surface or agriculture, reduces the occurrence probability of wild Brook Trout. Our results, however, also suggest that the presence of Brown Trout results in lower occurrence probability of Brook Trout over a range of anthropogenic landscape characteristics, compared with streams where Brown Trout were absent.

  14. Structured decision making for conservation of bull trout (Salvelinus confluentus) in Long Creek, Klamath River Basin, south-central Oregon

    Science.gov (United States)

    Benjamin, Joseph R.; McDonnell, Kevin; Dunham, Jason B.; Brignon, William R.; Peterson, James T.

    2017-06-21

    With the decline of bull trout (Salvelinus confluentus), managers face multiple, and sometimes contradictory, management alternatives for species recovery. Moreover, effective decision-making involves all stakeholders influenced by the decisions (such as Tribal, State, Federal, private, and non-governmental organizations) because they represent diverse objectives, jurisdictions, policy mandates, and opinions of the best management strategy. The process of structured decision making is explicitly designed to address these elements of the decision making process. Here we report on an application of structured decision making to a population of bull trout believed threatened by high densities of nonnative brook trout (S. fontinalis) and habitat fragmentation in Long Creek, a tributary to the Sycan River in the Klamath River Basin, south-central Oregon. This involved engaging stakeholders to identify (1) their fundamental objectives for the conservation of bull trout, (2) feasible management alternatives to achieve their objectives, and (3) biological information and assumptions to incorporate in a decision model. Model simulations suggested an overarching theme among the top decision alternatives, which was a need to simultaneously control brook trout and ensure that the migratory tactic of bull trout can be expressed. More specifically, the optimal management decision, based on the estimated adult abundance at year 10, was to combine the eradication of brook trout from Long Creek with improvement of downstream conditions (for example, connectivity or habitat conditions). Other top decisions included these actions independently, as well as electrofishing removal of brook trout. In contrast, translocating bull trout to a different stream or installing a barrier to prevent upstream spread of brook trout had minimal or negative effects on the bull trout population. Moreover, sensitivity analyses suggested that these actions were consistently identified as optimal across

  15. Invasion by nonnative brook trout in Panther Creek, Idaho: Roles of local habitat quality, biotic resistance, and connectivity to source habitats

    Science.gov (United States)

    Benjamin, Joseph R.; Dunham, Jason B.; Dare, M.R.

    2007-01-01

    Theoretical models and empirical evidence suggest that the invasion of nonnative species in freshwaters is facilitated through the interaction of three factors: habitat quality, biotic resistance, and connectivity. We measured variables that represented each factor to determine which were associated with the occurrence of nonnative brook trout Salvelinus fontinalis in Panther Creek, a tributary to the Salmon River, Idaho. Habitat variables included measures of summer and winter temperature, instream cover, and channel size. The abundance of native rainbow trout Oncorhynchus mykiss within sampled sites was used as a measure of biotic resistance. We also considered the connectivity of sample sites to unconfined valley bottoms, which were considered habitats that may serve as sources for the spread of established populations of brook trout. We analyzed the occurrence of small (<150‐mm [fork length]) and large (≥150‐mm) brook trout separately, assuming that the former represents an established invasion while accounting for the higher potential mobility of the latter. The occurrence of small brook trout was strongly associated with the proximity of sites to large, unconstrained valley bottoms, providing evidence that such habitats may serve as sources for the spread of brook trout invasion. Within sites, winter degree‐days and maximum summer temperature were positively associated with the occurrence of small brook trout. The occurrence of large brook trout was not related to any of the variables considered, perhaps due to the difficulty of linking site‐specific habitat factors to larger and more mobile individuals. The abundance of rainbow trout was not conclusively associated with the occurrence of either small or large brook trout, providing little support for the role of biotic resistance. Overall, our results suggest that source connectivity and local habitat characteristics, but not biotic resistance, influence the establishment and spread of

  16. Factors affecting competitive dominance of rainbow trout over brook trout in southern Appalachian streams: Implications of an individual-based model

    Energy Technology Data Exchange (ETDEWEB)

    Clark, M.E. [Univ. of Tennessee, Knoxville, TN (United States); Rose, K.A. [Oak Ridge National Lab., TN (United States)

    1997-01-01

    We used an individual-based model to examine possible explanations for the dominance of rainbow trout Oncorhynchus mykiss over brook trout Salvelinus fontinalis in southern Appalachian streams. Model simulations were used to quantify the effects on interspecific competition of (1) competitive advantage for feeding sites by rainbow trout, (2) latitudinal differences in stream temperatures, flows, and daylight, (3) year-class failures, (4) lower fecundity of brook trout, and (5) reductions in spawning habitat. The model tracks the daily spawning, growth, and survival of individuals of both species throughout their lifetime in a series of connected stream habitat units (pools, runs, or riffles). Average densities of each species based on 100-year simulations were compared for several levels of each of the five factors and for sympatric and allopatric conditions. Based on model results and empirical information, we conclude that more frequent year-class failures and the lower fecundity of brook trout are both possible and likely explanations for rainbow trout dominance, that warmer temperatures due to latitude and limited spawning habitat are possible but unlikely explanations, and that competitive advantage for feeding sites by rainbow trout is an unlikely explanation. Additional field work should focus on comparative studies of the reproductive success and the early life stage mortalities of brook and rainbow trout among Appalachian streams with varying rainbow trout dominance. 53 refs., 11 figs.

  17. Brook trout distributional response to unconventional oil and gas development: Landscape context matters

    Science.gov (United States)

    Merriam, Eric R.; Petty, J. Todd; Maloney, Kelly O.; Young, John A.; Faulkner, Stephen; Slonecker, Terry; Milheim, Lesley E.; Hailegiorgis, Atesmachew; Niles, Jonathan M.

    2018-01-01

    We conducted a large-scale assessment of unconventional oil and gas (UOG) development effects on brook trout (Salvelinus fontinalis) distribution. We compiled 2231 brook trout collection records from the Upper Susquehanna River Watershed, USA. We used boosted regression tree (BRT) analysis to predict occurrence probability at the 1:24,000 stream-segment scale as a function of natural and anthropogenic landscape and climatic attributes. We then evaluated the importance of landscape context (i.e., pre-existing natural habitat quality and anthropogenic degradation) in modulating the effects of UOG on brook trout distribution under UOG development scenarios. BRT made use of 5 anthropogenic (28% relative influence) and 7 natural (72% relative influence) variables to model occurrence with a high degree of accuracy [Area Under the Receiver Operating Curve (AUC) = 0.85 and cross-validated AUC = 0.81]. UOG development impacted 11% (n = 2784) of streams and resulted in a loss of predicted occurrence in 126 (4%). Most streams impacted by UOG had unsuitable underlying natural habitat quality (n = 1220; 44%). Brook trout were predicted to be absent from an additional 26% (n = 733) of streams due to pre-existing non-UOG land uses (i.e., agriculture, residential and commercial development, or historic mining). Streams with a predicted and observed (via existing pre- and post-disturbance fish sampling records) loss of occurrence due to UOG tended to have intermediate natural habitat quality and/or intermediate levels of non-UOG stress. Simulated development of permitted but undeveloped UOG wells (n = 943) resulted in a loss of predicted occurrence in 27 additional streams. Loss of occurrence was strongly dependent upon landscape context, suggesting effects of current and future UOG development are likely most relevant in streams near the probability threshold due to pre-existing habitat degradation.

  18. Temperature selection of goldfish (Carassius auratus L. ) and brook trout (Salvelinus fontinalis Mitch. ) after heterogeneous temperature acclimation

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, R.

    1977-01-01

    Goldfish and brook trout were acclimated heterogeneously to temperature by exposing the head and tail simultaneously to different temperatures. The temperature selection of heterogeneously acclimated fish was tested in a vertical temperature gradient and compared to that of homogeneously acclimated fish. All fish selected temperatures essentially corresponding to the state of acclimation of their head. It is concluded that the brain acts as the main control of temperature selection in fish.

  19. An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples

    Directory of Open Access Journals (Sweden)

    Laura Clusa

    2017-02-01

    Full Text Available Background Salmonids are native from the North Hemisphere but have been introduced for aquaculture and sport fishing in the South Hemisphere and inhabit most rivers and lakes in temperate and cold regions worldwide. Five species are included in the Global Invasive Species Database: rainbow trout Oncorhynchus mykiss, Atlantic salmon Salmo salar, brown trout Salmo trutta, brook trout Salvelinus fontinalis, and lake trout Salvelinus namaycush. In contrast, other salmonids are endangered in their native settings. Methods Here we have developed a method to identify salmonid species directly from water samples, focusing on the Iberian Peninsula as a case study. We have designed nested Salmonidae-specific primers within the 16S rDNA region. From these primers and a PCR-RFLP procedure the target species can be unequivocally identified from DNA extracted from water samples. Results The method was validated in aquarium experiments and in the field with water from watersheds with known salmonid populations. Finally, the method was applied to obtain a global view of the Salmonidae community in Nalón River (north coast of Spain. Discussion This new powerful, very sensitive (identifying the species down to 10 pg DNA/ml water and economical tool can be applied for monitoring the presence of salmonids in a variety of situations, from checking upstream colonization after removal of river barriers to monitoring potential escapes from fish farms.

  20. Genome evolution in the fish family salmonidae: generation of a brook charr genetic map and comparisons among charrs (Arctic charr and brook charr with rainbow trout

    Directory of Open Access Journals (Sweden)

    Moghadam Hooman K

    2011-07-01

    Full Text Available Abstract Background Salmonids are regarded as 4R derivative species, having experienced 4 whole genome duplication events in their ancestry. Many duplicated chromosome regions still share extensive homology with one another which is maintained primarily through male-based homeologous chromosome pairings during meiosis. The formation of quadrivalents during meiosis leads to pseudolinkage. This phenomenon is more prevalent within 5 of the 12 ancestral teleost linkage groups in salmonids. Results We constructed a genetic linkage map for brook charr and used this in combination with the genetic map from Arctic charr, to make comparisons with the genetic map of rainbow trout. Although not all chromosome arms are currently mapped, some homologous chromosome rearrangements were evident between Arctic charr and brook charr. Notably, 10 chromosome arms in brook charr representing 5 metacentric chromosomes in Arctic charr have undergone rearrangements. Three metacentrics have one arm translocated and fused with another chromosome arm in brook charr to a make a new metacentrics while two metacentrics are represented by 4 acrocentric pairs in brook charr. In two cases (i.e., BC-4 and BC-16, an apparent polymorphism was observed with the identification of both a putative metacentric structure (similar to metacentric AC-4 = BC-4 and a joining of acrocentric AC-16 + one arm of AC-28 = BC-16, as well as two separate acrocentric linkage groups evident in the mapping parents. Forty-six of the expected 50 karyotypic arms could be inter-generically assigned. SEX in brook charr (BC-4 was localized to the same homologous linkage group region as in Arctic charr (AC-4. The homeologous affinities detected in the two charr species facilitated the identification of 20 (expected number = 25 shared syntenic regions with rainbow trout, although it is likely that some of these regions were partial or overlapping arm regions. Conclusions Inter-generic comparisons among 2

  1. DECLINE AND EXTINCTION OF LAKE TROUT IN THE GREAT LAKES: CAN BIOLOGICAL INDICATORS HELP DIAGNOSE CAUSES, IDENTIFY REMEDIAL ACTIONS, AND PREDICT FUTURE CONDITIONS?

    Science.gov (United States)

    The lake trout, Salvelinus namaycush, is the predominant top predator native fish species of the Great Lakes. Lake trout are valued for commercial and recreational use in addition to their ecological importance. In the last half of the 20th century, population declines lead to vi...

  2. Ice processes affect habitat use and movements of adult cutthroat trout and brook trout in a Wyoming foothills stream

    Science.gov (United States)

    Lindstrom, J.W.; Hubert, W.A.

    2004-01-01

    Habitat use and movements of 25 adult cutthroat trout Oncorhynchus clarkii and 25 adult brook trout Salvelinus fontinalis from fall through winter 2002-2003 were assessed by means of radiotelemetry in a 7-km reach of a Rocky Mountains foothills stream. Temporal dynamics of winter habitat conditions were evaluated by regularly measuring the features of 30 pools and 5 beaver Castor canadensis ponds in the study reach. Groundwater inputs at three locations raised mean daily water temperatures in the stream channel during winter to 0.2-0.6??C and kept at least 250 m of the downstream channel free of ice, but the lack of surface ice further downstream led to the occurrence of frazil ice and anchor ice in pools and unstable habitat conditions for trout. Pools in segments that were not affected by groundwater inputs and beaver ponds tended to be stable and snow accumulated on the surface ice. Pools throughout the study reach tended to become more stable as snow accumulated. Both cutthroat trout and brook trout selected beaver ponds as winter progressed but tended to use lateral scour pools in proportion to their availability. Tagged fish not in beaver ponds selected lateral scour pools that were deeper than average and stable during winter. Movement frequencies by tagged fish decreased from fall through winter, but some individuals of both species moved during winter. Ice processes affected both the habitat use and movement patterns of cutthroat trout and brook trout in this foothills stream.

  3. Modeling brook trout presence and absence from landscape variables using four different analytical methods

    Science.gov (United States)

    Steen, Paul J.; Passino-Reader, Dora R.; Wiley, Michael J.

    2006-01-01

    As a part of the Great Lakes Regional Aquatic Gap Analysis Project, we evaluated methodologies for modeling associations between fish species and habitat characteristics at a landscape scale. To do this, we created brook trout Salvelinus fontinalis presence and absence models based on four different techniques: multiple linear regression, logistic regression, neural networks, and classification trees. The models were tested in two ways: by application to an independent validation database and cross-validation using the training data, and by visual comparison of statewide distribution maps with historically recorded occurrences from the Michigan Fish Atlas. Although differences in the accuracy of our models were slight, the logistic regression model predicted with the least error, followed by multiple regression, then classification trees, then the neural networks. These models will provide natural resource managers a way to identify habitats requiring protection for the conservation of fish species.

  4. Recent changes in the deep-water fish populations of Lake Michigan

    Science.gov (United States)

    Moffett, James W.

    1957-01-01

    The deep-water fish fauna of Lake Michigan consisted of lake trout (Salvelinus namaycush), burbot (Lota lota maculosa), seven species of chubs or deep-water ciscoes (Leucichthys spp.), and the deep-water sculpin (Myoxocephalus quadricornis). Other species occupied the deep-water zone but were not typically part of the fauna.

  5. Changes in seasonal climate outpace compensatory density-dependence in eastern brook trout

    Science.gov (United States)

    Bassar, Ronald D.; Letcher, Benjamin H.; Nislow, Keith H.; Whiteley, Andrew R.

    2016-01-01

    Understanding how multiple extrinsic (density-independent) factors and intrinsic (density-dependent) mechanisms influence population dynamics has become increasingly urgent in the face of rapidly changing climates. It is particularly unclear how multiple extrinsic factors with contrasting effects among seasons are related to declines in population numbers and changes in mean body size and whether there is a strong role for density-dependence. The primary goal of this study was to identify the roles of seasonal variation in climate driven environmental direct effects (mean stream flow and temperature) versus density-dependence on population size and mean body size in eastern brook trout (Salvelinus fontinalis). We use data from a 10-year capture-mark-recapture study of eastern brook trout in four streams in Western Massachusetts, USA to parameterize a discrete-time population projection model. The model integrates matrix modeling techniques used to characterize discrete population structures (age, habitat type and season) with integral projection models (IPMs) that characterize demographic rates as continuous functions of organismal traits (in this case body size). Using both stochastic and deterministic analyses we show that decreases in population size are due to changes in stream flow and temperature and that these changes are larger than what can be compensated for through density-dependent responses. We also show that the declines are due mostly to increasing mean stream temperatures decreasing the survival of the youngest age class. In contrast, increases in mean body size over the same period are the result of indirect changes in density with a lesser direct role of climate-driven environmental change.

  6. Ecology and impacts of nonnative salmonids with special reference to brook trout (Salvelinus fontinalis Mitchill) in North Europe

    OpenAIRE

    Korsu, K. (Kai)

    2008-01-01

    Abstract My main objectives in this thesis were to explore general patterns and mechanisms driving salmonid invasions globally and, more specifically, to examine the invasion dynamics and impacts of the North American brook trout in North European stream systems. Non-native salmonids have often spread extensively and caused many harmful impacts on their native counterparts. Among the three globally introduced salmonids, the European brown trout appeared as the 'worst' alien species (st...

  7. Bilan des introductions de salmonidés dans les lacs et ruisseaux d'altitude des Hautes-Pyrénées

    Directory of Open Access Journals (Sweden)

    DELACOSTE M.

    1997-01-01

    Full Text Available Les introductions de Salmonidés ont été importantes au cours des 60 dernières années dans les lacs et ruisseaux d'altitude des Hautes-Pyrénées. Six espèces de Salmonidés ont été introduites dans des milieux qui, pour la plupart, étaient vierges de populations piscicoles : la truite commune (Salmo trutta L., la truite arc-en-ciel (Oncorhynchus mykiss Walbaum, l'omble de fontaine (Salvelinus fontinalis Mitchill, l'omble chevalier (Salvelinus alpinus L., le cristivomer (Salvelinus namaycush Walbaum et le splake (Salvelinus fontinalis x Salvelinus namaycush. Dans de très nombreux cas, ces introductions ont abouti à des acclimatations. En revanche, les naturalisations sont beaucoup plus rares. Seules les espèces lacustres (cristivomer et omble chevalier se sont naturalisées dans la majorité des lacs où elles ont été introduites. Les conditions de reproduction constituent le facteur clé permettant d'expliquer la naturalisation des espèces. En ruisseau, il faut y ajouter la compétition avec l'espèce indigène (la truite commune, la pression halieutique ainsi que les conditions hivernales très rigoureuses. Les incidences écologiques des introductions sur les populations de truites communes indigènes sont faibles. En revanche, elles ne sont pas négligeables pour les populations de batraciens. Cette politique d'introduction a largement participé au développement de l'halieutisme dans ces milieux d'altitude. En cela, les introductions ont parfaitement répondu aux objectifs halieutiques qu'on leur avait fixés. L'acquisition de connaissances sur l'ensemble de la chaîne pyrénéenne constitue aujourd'hui une étape incontournable pour une politique de gestion globale des introductions.

  8. Genome Sequences of Shewanella baltica and Shewanella morhuae Strains Isolated from the Gastrointestinal Tract of Freshwater Fish.

    Science.gov (United States)

    Castillo, Daniel; Gram, Lone; Dailey, Frank E

    2018-06-21

    We present here the genome sequences of Shewanella baltica strain CW2 and Shewanella morhuae strain CW7, isolated from the gastrointestinal tract of Salvelinus namaycush (lean lake trout) and Coregonus clupeaformis (whitefish), respectively. These genome sequences provide insights into the niche adaptation of these specific species in freshwater systems. Copyright © 2018 Castillo et al.

  9. Thermal onset of cellular and endocrine stress responses correspond to ecological limits in brook trout, an iconic cold-water fish

    Science.gov (United States)

    Chadwick, Joseph G; Nislow, Kieth H; McCormick, Stephen

    2015-01-01

    Climate change is predicted to change the distribution and abundance of species, yet underlying physiological mechanisms are complex and methods for detecting populations at risk from rising temperature are poorly developed. There is increasing interest in using physiological mediators of the stress response as indicators of individual and population-level response to environmental stressors. Here, we use laboratory experiments to show that the temperature thresholds in brook trout (Salvelinus fontinalis) for increased gill heat shock protein-70 (20.7°C) and plasma glucose (21.2°C) are similar to their proposed thermal ecological limit of 21.0°C. Field assays demonstrated increased plasma glucose, cortisol and heat shock protein-70 concentrations at field sites where mean daily temperature exceeded 21.0°C. Furthermore, population densities of brook trout were lowest at field sites where temperatures were warm enough to induce a stress response, and a co-occurring species with a higher thermal tolerance showed no evidence of physiological stress at a warm site. The congruence of stress responses and proposed thermal limits supports the use of these thresholds in models of changes in trout distribution under climate change scenarios and suggests that the induction of the stress response by elevated temperature may play a key role in driving the distribution of species.

  10. The physiological stress response and oxidative stress biomarkers in rainbow trout and brook trout from selenium-impacted streams in a coal mining region

    Energy Technology Data Exchange (ETDEWEB)

    Miller, L.L.; Rasmussen, J.B.; Palace, V.P.; Hontela, A. [University of Lethbridge, Lethbridge, AB (Canada). Dept. of Biological Science

    2009-11-15

    Selenium (Se) is an essential element that can be toxic at concentrations slightly greater than those required for homeostasis. The main chronic toxic effects of Se in fish are teratogenic deformities, but Se can also activate the physiological stress response and redox cycle with reduced glutathione causing oxidative damage. Rainbow trout, Oncorhynchus mykiss, appear to be more sensitive to Se than brook trout, Salvelinus fontinalis. The objective of this study was to compare the physiological stress response (plasma cortisol, glucose, triiodothyronine, thyroxine, gill Na+/K+ ATPase, cortisol secretory capacity, K and liver somatic index) and oxidative stress biomarkers (liver GSH, GPx, lipid peroxidation, vitamin A and vitamin E) in rainbow trout (RNTR) and brook trout (BKTR) collected from reference and Se-exposed streams. The physiological stress response was not impaired (cortisol secretory capacity unchanged); although there were species differences in plasma cortisol and plasma glucose levels. Liver GSH, GPx and vitamin levels were higher in RNTR than BKTR, but lipid peroxidation levels were not different. The elevated GSH reserves may make RNTR more sensitive to Se-induced lipid peroxidation, but this may be offset by the RNTR's higher antioxidant (GPx and vitamin) levels. Species-specific biochemical differences may mediate differences in Se sensitivity and be used in aquatic Se risk assessments.

  11. Species succession and sustainability of the Great Lakes fish community

    Science.gov (United States)

    Eshenroder, Randy L.; Burnham-Curtis, Mary K.; Taylor, William W.; Ferreri, C. Paola

    1999-01-01

    This article concentrates on the sustainability of the offshore pelagic and deepwater fish communities that were historically dominated by lake trout (Salvelinus namaycush). The causes of alteration in these fish communities (i.e., overfishing, introductions, and cultural eutrophication) were identified by Loftus and Regier (1972). Here we look at the ecology of these altered communities in relation to sustainability and discuss the need for restoration.

  12. Harriet Brooks-Pioneer nuclear scientist

    Science.gov (United States)

    Rayner-Canham, M. F.; Rayner-Canham, G. W.

    1989-10-01

    This article, using revealing statements from contemporary correspondence, traces the eventful life of Harriet Brooks, one of Ernest Rutherford's most valued research students and collaborators at McGill University. Brooks performed some of the crucial experiments in the early work on radioactivity; her work led her to the Cavendish where she did work with J. J. Thomson. Still later, she worked with Marie Curie, to whom Rutherford favorably compared her. Despite Brooks' achievements and promise, she finally relinquished her research career when faced with insurmountable objections to women who wished to have both a professional and a married life.

  13. Role of climate and invasive species in structuring trout distributions in the interior Columbia River Basin, USA

    Science.gov (United States)

    Wenger, Seth J.; Isaak, Daniel J.; Dunham, Jason B.; Fausch, Kurt D.; Luce, Charles H.; Neville, Helen M.; Rieman, Bruce E.; Young, Michael K.; Nagel, David E.; Horan, Dona L.; Chandler, Gwynne L.

    2011-01-01

    Recent and projected climate warming trends have prompted interest in impacts on coldwater fishes. We examined the role of climate (temperature and flow regime) relative to geomorphology and land use in determining the observed distributions of three trout species in the interior Columbia River Basin, USA. We considered two native species, cutthroat trout (Oncorhynchus clarkii) and bull trout (Salvelinus confluentus), as well as nonnative brook trout (Salvelinus fontinalis). We also examined the response of the native species to the presence of brook trout. Analyses were conducted using multilevel logistic regression applied to a geographically broad database of 4165 fish surveys. The results indicated that bull trout distributions were strongly related to climatic factors, and more weakly related to the presence of brook trout and geomorphic variables. Cutthroat trout distributions were weakly related to climate but strongly related to the presence of brook trout. Brook trout distributions were related to both climate and geomorphic variables, including proximity to unconfined valley bottoms. We conclude that brook trout and bull trout are likely to be adversely affected by climate warming, whereas cutthroat trout may be less sensitive. The results illustrate the importance of considering species interactions and flow regime alongside temperature in understanding climate effects on fish.

  14. Use of wild trout for PBDE assessment in freshwater environments: Review and summary of critical factors

    Directory of Open Access Journals (Sweden)

    Juan M. Ríos

    2015-11-01

    Full Text Available Certain wild animals represent sentinels to address issues related to environmental pollution, since they can provide integrative data on both pollutant exposure and biological effects. Despite their technological benefits, PBDEs are considered a threat to environmental health due to their persistence, toxicity, and capacity to be accumulated. These pollutants have been found geographically widespread in fish, particularly in predator species such as trout. The aim of this work is to critically review the applicability and usefulness of wild trout for assessing PBDEs in freshwater environments. Reviewed reports include data from highly industrialized areas as well as areas from remote regions with relatively low human activity, including European and North American great lakes and freshwater environments in Europe, Greenland, subarctic areas and Patagonia, respectively. A summary of relevant factors were grouped into organism-specific factors (food habits, age, size, lipid content, sex and reproduction, tissue type, mechanism of contaminant uptake and metabolism, and PBDE levels in the surrounding environment (sediment. Five wild trout species [rainbow trout (Oncorhynchus mykiss, brown trout (Salmo trutta, lake trout (Salvelinus namaycush, arctic char (Salvelinus alpinus, and brook trout (Salvelinus fontinalis], collected worldwide within the 1994 to present time frame, were considered. Multivariate techniques (principal component analysis-PCA and mapping approach, showed clear differences in geographic distribution patterns of PBDE levels in trout depending on the region studied: wild trout from European and North American great lakes have the highest PBDE loads. This pattern could be due to high industrial activity at these locations. A correlational approach used to explore intraspecific relationships between PBDE levels and morphometry, showed positive relationships only for brown trout. Further, brown trout showed the highest trout

  15. Polychlorinated biphenyl reduction in lake trout by irradiation and broiling

    International Nuclear Information System (INIS)

    Cichy, R.F.; Zabik, M.E.; Weaver, C.M.

    1979-01-01

    The Great Lakes Environmental contaminants surveys (1974-1975) have concluded that excessive concentrations of polychlorinated biphenyls (PCBs) are present in a specific species of lake trout (Salvelinus namaycush). Over 30 ppM of PCBs have been detected in the edible fillet of this fat trout. The objective of this study was to determine the effect of gamma irradiation combined with broiling on the levels of PCBs in lake trout fillets

  16. First evidence of successful natural reproduction by planted lake trout in Lake Huron

    Science.gov (United States)

    Nester, Robert T.; Poe, Thomas P.

    1984-01-01

    Twenty-two lake trout (Salvelinus namaycush) swim-up fry, 24-27 mm long, were captured with emergent fry traps and a tow net in northwestern Lake Huron on a small nearshore reef off Alpena, Michigan, between May 10 and June 1, 1982. These catches represent the first evidence of successful production of swim-up fry by planted, hatchery-reared lake trout in Lake Huron since the lake trout rehabilitation program began in 1973.

  17. Esterification and hydrolysis of vitamin A in the liver of brook trout (Salvelinus fontinalis) and the influence of a coplanar polychlorinated biphenyl

    International Nuclear Information System (INIS)

    Ndayibagira, A.; Spear, P.A.

    1999-01-01

    Recent reports of extremely low retinoid stores in fish living in contaminated river systems prompted an initial investigation of the mechanisms of hepatic storage and mobilization in brook trout. Enzyme characterization in microsomes revealed a lecithin:retinol acyltransferase activity (LRAT) optimum in the alkaline range (pH 9.0; V max =0.6 nmol per mg prot. h -1 ; K m =10.2 μM) which is not known to occur in mammals, in addition to a secondary optimum at pH 6.5 typical of mammals. Acyl CoA:retinol acyltransferase (ARAT) kinetic parameters were quite different to those of mammals. The substrate affinity of trout ARAT (K m =1.6 μM) was approximately 22-fold greater than that of the rat while maximal velocity (V max =0.2 nmol per mg prot. h -1 ) was 18-fold less. Retinyl ester hydrolase activity (REH) was optimal under acid conditions (pH 4.2; V max =6.6 nmol per mg prot. h -1 ; K m =0.6 mM), was inhibited by a bile salt analogue and was greater in males than females. This REH was tentatively categorized as a bile salt-independent, acid retinyl ester hydrolase (BSI-AREH). REH was inhibited in a dose-dependent manner following in vivo exposure to a representative environmental contaminant the coplanar polychlorinated biphenyl (PCB), 3,3minutes or feet,4,4minutes or feet-tetrachlorobiphenyl (TCBP). Inhibition may be an indirect effect because enzyme activity was not affected by in vitro exposure of control microsomes. REH inhibition in the brook trout may affect the uptake of retinyl esters (REs) from chylomicron remnants as well as the mobilization of stored REs. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  18. Utilization of soya protein as an alternative protein source in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-01-05

    Jan 5, 2009 ... For carcass trait, ash, crude fat, and energy varied significantly with soya protein ... high-protein content, relatively well-balanced amino acid profile ..... and organoleptic quality of flesh of brook char (Salvelinus fontinalis).

  19. The reproduction of lake trout in southern Lake Superior

    Science.gov (United States)

    Eschmeyer, Paul H.

    1955-01-01

    The principal spawning grounds of lake trout (Salvelinus namaycush namaycush) in United States waters of southern Lake Superior are on rocky shoals at depths of less than 20 fathoms. Most spawning occurs in October and early November. Of the mature fish collected on or near the spawning grounds, 60 to 69 percent were males. Among mature fish the average length of females was greater than that of males; few males less than 24 inches or females less than 26 inches in total length were caught. Recoveries of lake trout tagged on the spawning grounds showed that some males remained in the immediate area for a period of several weeks during the spawning season. Marked fish showed a tendency to return during later years to spawning grounds on which they had been tagged, even though many of them ranged long distances between spawning seasons.

  20. THE BEAR BROOK WATERSHED MANIPULATION PROJECT: WATERSHED SCIENCE IN A POLICY PERSPECTIVE

    Science.gov (United States)

    The Bear Brook Watershed Manipulation in Maine is a paired watershed experiment. Monitoring of the paired catchments (East Bear Brook - reference; West Bear Brook - experimental) began in early 1987. Chemical manipulation of West Bear Brook catchment began in November 1989. Proce...

  1. Dose-dependent stimulation of hepatic retinoic acid hydroxylation/oxidation and glucuronidation in brook trout, Salvelinus fontinalis, after exposure to 3,3{prime}, 4,4{prime}-tetrachlorobiphenyl

    Energy Technology Data Exchange (ETDEWEB)

    Boyer, P.M.; Ndayibagira, A.; Spear, P.A.

    2000-03-01

    Extremely low stores of vitamin A have been reported in fish and birds inhabiting regions contaminated by coplanar polychlorinated biphenyls (PCBs) and other organochlorines, suggesting many possible effects on retinoid biochemical pathways. Metabolic imbalances associated with biologically active retinoids (e.g., retinoic acid) could be associated with tetratogenesis, edema, growth inhibition, reproductive impairment, immunosuppression, and susceptibility to cancer. Sexually mature brook trout were injected imtraperitoneally with the coplanar PCB 3,3{prime}, 4,4{prime}-tetrachlorobiphenyl (TCBP) and again 4 weeks later. At 8 weeks, retinoic acid metabolism was measured in liver microsomes. To the authors' knowledge, retinoic acid conjugation by UDP-glucuronyltransferase is described here for the first time in fish. A substantial rate of glucuronidation was detected in the microsomes from control brook trout, which tended to increase over the dose range of TCBP. Glucuronidation was significantly greater in fish receiving the 10 {micro}g/g body weight dose level. Metabolism through the cytochrome P450 system was also dose-dependent, resulting in significantly greater production of 4-hydroxyretinoic acid at the 10 {micro}g/g dose level. In contrast, subsequent oxidation to 4-oxo-retinoic acid was greatest at the 1 {micro}g/g dose level and did not increase further at higher doses. Liver stores of dehydroretinyl palmitate/oleate were significantly decreased at the 5 and 10 {micro}g/g dose levels.

  2. Hydraulic modeling of stream channels and structures in Harbor and Crow Hollow Brooks, Meriden, Connecticut

    Science.gov (United States)

    Weiss, Lawrence A.; Sears, Michael P.; Cervione, Michael A.

    1994-01-01

    Effects of urbanization have increased the frequency and size of floods along certain reaches of Harbor Brook and Crow Hollow Brook in Meriden, Conn. A floodprofile-modeling study was conducted to model the effects of selected channel and structural modifications on flood elevations and inundated areas. The study covered the reach of Harbor Brook downstream from Interstate 691 and the reach of Crow Hollow Brook downstream from Johnson Avenue. Proposed modifications, which include changes to bank heights, channel geometry, structural geometry, and streambed armoring on Harbor Brook and changes to bank heights on Crow Hollow Brook, significantly lower flood elevations. Results of the modeling indicate a significant reduction of flood elevations for the 10-year, 25-year, 35-year, 50-year, and 100-year flood frequencies using proposed modifications to (1 ) bank heights between Harbor Brook Towers and Interstate 691 on Harbor Brook, and between Centennial Avenue and Johnson Avenue on Crow Hollow Brook; (2) channel geometry between Coe Avenue and Interstate 69 1 on Harbor Brook; (3) bridge and culvert opening geometry between Harbor Brook Towers and Interstate 691 on Harbor Brook; and (4) channel streambed armoring between Harbor Brook Towers and Interstate 691 on Harbor Brook. The proposed modifications were developed without consideration of cost-benefit ratios.

  3. Species-specific sensitivity to selenium-induced impairment of cortisol secretion in adrenocortical cells of rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis)

    International Nuclear Information System (INIS)

    Miller, L.L.; Hontela, A.

    2011-01-01

    Species differences in physiological and biochemical attributes exist even among closely related species and may underlie species-specific sensitivity to toxicants. Rainbow trout (RT) are more sensitive than brook trout (BT) to the teratogenic effects of selenium (Se), but it is not known whether all tissues exhibit this pattern of vulnerability. In this study, primary cultures of RT and BT adrenocortical cells were exposed to selenite (Na 2 SO 3 ) and selenomethionine (Se-Met) to compare cell viability and ACTH-stimulated cortisol secretion in the two fish species. Cortisol, the primary stress hormone in fish, facilitates maintenance of homeostasis when fish are exposed to stressors, including toxicants. Cell viability was not affected by Se, but selenite impaired cortisol secretion, while Se-Met did not (RT and BT EC 50 > 2000 mg/L). RT cells were more sensitive (EC 50 = 8.7 mg/L) to selenite than BT cells (EC 50 = 90.4 mg/L). To identify the targets where Se disrupts cortisol synthesis, selenite-impaired RT and BT cells were stimulated with ACTH, dbcAMP, OH-cholesterol, and pregnenolone. Selenite acted at different steps in the cortisol biosynthesis pathway in RT and BT cells, confirming a species-specific toxicity mechanism. To test the hypothesis that oxidative stress mediates Se-induced toxicity, selenite-impaired RT cells were exposed to NAC, BSO and antioxidants (DETCA, ATA, Vit A, and Vit E). Inhibition of SOD by DETCA enhanced selenite-induced cortisol impairment, indicating that oxidative stress plays a role in Se toxicity; however, modifying GSH content of the cells did not have an effect. The results of this study, with two closely related salmonids, provided additional evidence for species-specific differences in sensitivity to Se which should be considered when setting thresholds and water quality guidelines. - Research Highlights: → We investigated species-specific sensitivity to Se in trout adrenocortical cells. → Selenite, not Se-Met, disrupts

  4. Altered steroid metabolism in several teleost species exposed to endocrine disrupting substances in refuse dump leachate

    NARCIS (Netherlands)

    Noaksson, E.; Linderoth, M.; Bosveld, A.T.C.; Balk, L.

    2003-01-01

    Endocrine disruption associated with reproductive failure has been reported previously in female perch (Perca fluviatilis) and roach (Rutilus rutilus) from Lake Molnbyggen in Sweden and in female brook trout (Salvelinus fontinalis) from Vadbäcken, a stream emptying into Molnbyggen. Both Molnbyggen

  5. Aerospace medicine at Brooks AFB, TX: hail and farewell.

    Science.gov (United States)

    Nunneley, Sarah A; Webb, James T

    2011-05-01

    With the impending termination of USAF operations at Brooks Air Force Base (AFB) in San Antonio, TX, it is time to consider its historic role in Aerospace Medicine. The base was established in 1917 as a flight training center for the U.S. Army Air Service and in 1926 became home to its School of Aviation Medicine. The school moved to San Antonio's Randolph Field in 1931, but in 1959 it returned to Brooks where it occupied new facilities to support its role as a national center for U.S. Air Force aerospace medicine, including teaching, clinical medicine, and research. The mission was then expanded to encompass support of U.S. military and civilian space programs. With the abrupt termination of the military space program in 1969, research at Brooks focused on clinical aviation medicine and support of advanced military aircraft while continuing close cooperation with NASA in support of orbital spaceflight and the journey to the Moon. Reorganization in the 1990s assigned all research functions at Brooks to the Human Systems Division and its successors, leaving to USAFSAM the missions related to clinical work and teaching. In 2002 the USAF and the city of San Antonio implemented shared operation of Brooks as a "City-Base" in the hope of deflecting threatened closure. Nevertheless, under continuing pressure to consolidate military facilities in the United States, the 2005 Base Closure and Realignment Commission ordered Brooks closed by 2011, with its aerospace medicine functions relocated to new facilities at Wright-Patterson AFB in Dayton, OH.

  6. Reframing Romaine Brooks' heroic queer modernism.

    Science.gov (United States)

    Langer, Cassandra L

    2010-01-01

    Modernism was not a wholesale embracing of Greenberg's definition as abstracting, non-objective, and autonomous. The expatriate U.S. artist and lesbian Romaine Brooks politicized her portraits of females based on a queer combination of the Byronic erotic and Baudelaire's modern dandy. Her execution of her queer modernist aesthetics re-presents female heroes as part of a self-reflective dynamic of lesbian modernity that emphasizes the ambiguity of normative gender binaries and plays with style, personality, and impersonation as disrupting to bourgeoisie mores. My focus is on how Brooks shatters normative conventions of portraiture in her revolutionary critique of heteronormativity.

  7. Katherine Brooke'ist / Tiina Lepiste

    Index Scriptorium Estoniae

    Lepiste, Tiina

    2007-01-01

    Ameerika teleseriaali "Vaprad ja ilusad" ("The Bold and the Beautiful") osatäitja Katherine Kelly Lang (Brooke). Artikli aluseks on Soap Opera Weekly ajakirjaniku Linda Susmani vestlus näitlejannaga

  8. Pressure shock triploidization of Salmo trutta f. lacustris and Salvelinus umbla eggs and its impact on fish development.

    Science.gov (United States)

    Lahnsteiner, Franz; Kletzl, Manfred

    2018-07-15

    The study tested the efficiency of hydrostatic pressure triploidization methods for Salmo trutta f. lacustris and Salvelinus umbla and investigated the effects on survival rate, skeletal malformation, and on morphometrics and cellular composition of gills, spleen, liver, kidney, intestine, and blood. In Salmo trutta f. lacustris a 100% triploidy rate in combination with high larvae survival rate (80% in comparison to control) was obtained when treating eggs with a pressure of 66 × 10 3  kPa 360 °C temperature minutes (CTM) post fertilization for 5 min, in Salvelinus umbla with a similar pressure after 270 CTM. Juvenile triploid Salmo trutta f. lacustris and Salvelinus umbla (145 days post hatch) had neither an increased rate of mortality, nor an increased rate of malformations. In triploid Salmo trutta f. lacustris and Salvelinus umbla the erythrocyte volume was 50% higher and the erythrocyte concentration in peripheral blood 25-35% lower relative to diploids. In triploids also the erythrocytes surface area: volume ratio was reduced. Gills of triploid Salmo trutta f. lacustris and Salvelinus umbla had increased width of primary lamellae and increased length of secondary lamellae which might compensate for unfavorable erythrocytes surface area: volume ratio. Length of the digestive tract and histology of kidney, liver, spleen, and gills were only investigated in Salmo trutta f. lacustris. In triploids the hematopoietic tissue of the kidney was decreased by 12%, the spleen index by 53%, and the erythroblast concentrations of the spleen by 42% relative to diploids, possibly indicating reduced erythropoiesis. Length of the digestive tract and cellular arrangement of intestine, liver, and gills were not affected. In summary, the used triploidization procedure seems a reliable method not counteracting the principles of animal welfare. Copyright © 2018 Elsevier Inc. All rights reserved.

  9. Genetic Diversity and Hybridisation between Native and Introduced Salmonidae Fishes in a Swedish Alpine Lake.

    Directory of Open Access Journals (Sweden)

    Leanne Faulks

    Full Text Available Understanding the processes underlying diversification can aid in formulating appropriate conservation management plans that help maintain the evolutionary potential of taxa, particularly under human-induced activities and climate change. Here we assessed the microsatellite genetic diversity and structure of three salmonid species, two native (Arctic charr, Salvelinus alpinus and brown trout, Salmo trutta and one introduced (brook charr, Salvelinus fontinalis, from an alpine lake in sub-arctic Sweden, Lake Ånn. The genetic diversity of the three species was similar and sufficiently high from a conservation genetics perspective: corrected total heterozygosity, H'T = 0.54, 0.66, 0.60 and allelic richness, AR = 4.93, 5.53 and 5.26 for Arctic charr, brown trout and brook charr, respectively. There were indications of elevated inbreeding coefficients in brown trout (GIS = 0.144 and brook charr (GIS = 0.129 although sibling relationships were likely a confounding factor, as a high proportion of siblings were observed in all species within and among sampling locations. Overall genetic structure differed between species, Fst = 0.01, 0.02 and 0.04 in Arctic charr, brown trout and brook charr respectively, and there was differentiation at only a few specific locations. There was clear evidence of hybridisation between the native Arctic charr and the introduced brook charr, with 6% of individuals being hybrids, all of which were sampled in tributary streams. The ecological and evolutionary consequences of the observed hybridisation are priorities for further research and the conservation of the evolutionary potential of native salmonid species.

  10. A Regional View of the Margin: Salmonid Abundance and Distribution in the Southern Appalachian Mountains of North Carolina and Virginia

    Science.gov (United States)

    Patricia A. Flebbe

    1994-01-01

    In the southern Appalachian Mountains, native brook trout Salvelinus fontinalis and introduced rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta are at the southern extremes of their distributions, an often overlooked kind of marginal habitat. At a regional scale composed of the states of Virginia...

  11. A Feasibility Study of Response Techniques for Discharges of Hazardous Chemicals That Disperse through the Water Column

    Science.gov (United States)

    1976-07-01

    relatively normal except fungi frequent. 5.5-6.0 Eastern brook trout (Salvelinus fontinalis) survive at over pit 5.5. Rainbow trout (Salmo gairdneri...salt or ester of a thio acid, made from corn or wheat starch. These material3 have the advantage of being non-toxic natural substances and potentially

  12. Characterization of a Neochlamydia-like Bacterium Associated with Epitheliocystis in Cultured Artic Char Salvelinus alpinus

    Science.gov (United States)

    Infections of branchial epithelium by intracellular gram-negative bacteria, termed epitheliocystis, have limited culture of Arctic char (Salvelinus alpinus). To characterize a bacterium associated with epitheliocystis in cultured char, gills were sampled for histopathologic examination, conventional...

  13. LopheliaII2012: Coral Research on Oil Rigs in the Gulf of Mexico on TDI-Brooks Vessel Brooks McCall between 2012-07-12 and 2012-07-24

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The final year of a multi-year effort to study Lophelia coral communities in the Gulf of Mexico is occurring on the TDI-Brooks research vessel, Brooks McCall,...

  14. 2012 USGS Lidar: Brooks Camp (AK)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. Geological Survey (USGS) had a requirement for high resolution Lidar needed for mapping the Brooks Camp region of Katmai National Park in Alaska....

  15. The Why, What, and Impact of GPA at Oxford Brookes University

    Science.gov (United States)

    Andrews, Matthew

    2016-01-01

    This paper examines the introduction at Oxford Brookes University of a Grade Point Average (GPA) scheme alongside the traditional honours degree classification. It considers the reasons for the introduction of GPA, the way in which the scheme was implemented, and offers an insight into the impact of GPA at Brookes. Finally, the paper considers…

  16. Climate change and vulnerability of bull trout (Salvelinus confluentus ) in a fire-prone landscape

    Science.gov (United States)

    Jeffrey A. Falke; Rebecca L. Flitcroft; Jason B. Dunham; Kristina M. McNyset; Paul F. Hessburg; Gordon H. Reeves; C. Tara Marshall

    2015-01-01

    Linked atmospheric and wildfire changes will complicate future management of native coldwater fishes in fire-prone landscapes, and new approaches to management that incorporate uncertainty are needed to address this challenge. We used a Bayesian network (BN) approach to evaluate population vulnerability of bull trout (Salvelinus confluentus) in the Wenatchee River...

  17. Physicochemical Analysis of Water Quality of Brook Kuruçay

    Directory of Open Access Journals (Sweden)

    Ekrem Mutlu

    2016-11-01

    Full Text Available In this study, through the analyses of water samples taken from 9 stations on the brook between July 2012 and June 2013, we aimed to determine the monthly and seasonal changes in water quality parameters of Brook Kuruçay, to determine the water quality properties, to reveal the pollution problems, to determine the suitability level in terms of aquatic life and to classify the quality of water in accordance with Surface Water Quality Management Regulation’s Inland Surface Water Classes criteria. The study area is located southeast of the Hafik District of Sivas city and the altitude is 2608 m. The water samples were collected from 9 stations established on the brook, and some physicochemical parameters and heavy metal concentrations were analyzed in water samples. The cleaning and maintenance of all of the equipment, land-type measurement tools, and glass sampling containers to be used in sampling were made 1 day before sampling. Sampling tubes were immersed into 15 cm below the water surface for taking water samples. Heavy metal concentrations were determined in the Sivas Provincial Control Laboratory in the same day with sampling (within 5 hours. The total alkalinity, total hardness, ammonium nitrogen, nitrite, nitrate, ammonium azote, phosphate, sulfite, sulfate, chloride, sodium, potassium, suspended solid matter (SSM, chemical oxygen demand (COD, biological oxygen demand (BOD, calcium, magnesium, ferrous, lead, copper, zinc, nickel, mercury and cadmium analyses of water samples were performed. As a result of the analyses, it was determined that, since Brook Kuruçay falls into the water resource class, which is the most sensitive to pollution, the water quality of the brook should be monitored regularly.

  18. Harriet Brooks: Canada's First Woman Physicist

    Science.gov (United States)

    Rayner-Canham, Geoffrey

    2004-03-01

    During those early halcyon days of the study of radioactivity, one young Canadian woman, Harriet Brooks, joined Ernest Rutherford's group as his first research student. Later, she joined J.J. Thomson's group in Cambridge and, finally, Marie Curie's group in Paris. During her short research career, she made several important contributions to science. She investigated the nature of 'emanation' from radium; discovered that radioactive substances could undergo successive decay; and first reported the recoil of the radioactive atom. Much of this research was published under her name alone though Rutherford made extensive reference to her discoveries in his Bakerian lecture of 1904. Brooks life is of interest not only in what she accomplished, but also in the challenges she faced as a pioneering woman scientist in the early part of the twentieth century. In the presentation we will blend the account of her life and work with the societal context. This work was accomplished jointly with Marelene F. Rayner-Canham.

  19. Metallothionein in brook trout (Salvenlinus fontinalis) as a biological indicator of cadmium stress

    International Nuclear Information System (INIS)

    Hamilton, S.J.; Mehrle, P.M.

    1987-01-01

    A cadmium-saturation technique for quantifying metallothionein in mammalian tissues was evaluated for use in fish tissue. Metallothionein characteristically binds 7 gram-atoms of a metal such as cadmium per mole of protein so saturating MT with respect to one metal and then quantifying that metal would thus result in the indirect quantification of MT. The authors administered 3 mg 109 cadmium/kg body weight by intraperitoneal injection over a 5-day period to adult brook trout Salvelinus fontinalis to induce MT in liver and kidney tissues. Homogenates were centrifuged and the supernatant was used to quantitate cadmium in three fractions: 100,000 g supernatant, cadmium-saturated MT, and unsaturated MT. The cadmium-saturated MT method involved the following steps: saturation of MT in an aliquot of 100,000 g supernatant with excess cadmium; removal of excess cadmium by addition of 2% hemoglobin; denaturation of hemoglobin by heating at 100 0 C followed by rapid cooling on ice; centrifugation at 10,000 g; digestion of an aliquot of supernatant in concentrated nitric acid for 16 hours at 70 0 C, and quantification of cadmium by atomic absorption and graphite furnace techniques or radiometric measurement with a scintillation counter. The cadmium saturation technique was modified in two ways so the amount of cadmium bound to unsaturated MT could be measured; first, the binding sites on MT were not saturated with excess cadmium, and second, the concentration of hemoglobin added to remove free cadmium and aid in coagulating low-molecular-weight proteins was 1% instead of 2%. The method gave precise measurements of MT concentrations when aliquots of liver homogenate which were analyzed separately were quantified by atomic absorption or radiometric measurements. Two to four times more cadmium and MT concentrated in the liver of treated fish than in the kidney

  20. HPLC and ELISA analyses of larval bile acids from Pacific and western brook lampreys

    Science.gov (United States)

    Yun, S.-S.; Scott, A.P.; Bayer, J.M.; Seelye, J.G.; Close, D.A.; Li, W.

    2003-01-01

    Comparative studies were performed on two native lamprey species, Pacific lamprey (Lampetra tridentata) and western brook lamprey (Lampetra richardsoni) from the Pacific coast along with sea lamprey (Petromyzon marinus) from the Great Lakes, to investigate their bile acid production and release. HPLC and ELISA analyses of the gall bladders and liver extract revealed that the major bile acid compound from Pacific and western brook larval lampreys was petromyzonol sulfate (PZS), previously identified as a migratory pheromone in larval sea lamprey. An ELISA for PZS has been developed in a working range of 20pg-10ng per well. The tissue concentrations of PZS in gall bladder were 127.40, 145.86, and 276.96??g/g body mass in sea lamprey, Pacific lamprey, and western brook lamprey, respectively. Releasing rates for PZS in the three species were measured using ELISA to find that western brook and sea lamprey released PZS 20 times higher than Pacific lamprey did. Further studies are required to determine whether PZS is a chemical cue in Pacific and western brook lampreys. ?? 2003 Elsevier Inc. All rights reserved.

  1. Final Environmental Impact Statement, Brooks City Base Project, Brooks Air Force Base, Texas

    Science.gov (United States)

    2001-03-01

    Maitland Road and the east base boundary (Goliad Road). The CoSA Fire Department Training Academy may be included within this area. The Fire Department...replacement of the gas lines in the 1100s area (the area containing Facilities 1162 through 1193 bounded by Welch, Chennault, Hawks, and Maitland roads...3 Ian Smith, P.E., REM, Brooks City Base Program Office, 311 HSW/CDB M.S., 1990, Civil Engineering, University of Nebraska B.C.E., 1978, Civil

  2. A raptor study for the proposed Grandy Brook Station to Hope Brook Mine transmission line environmental impact statement

    Energy Technology Data Exchange (ETDEWEB)

    1986-01-01

    The proposed Grandy Brook Station to Hope Brook mine transmission line study area occupies a remote area of south-central Newfoundland. Raptors have been identified as a valued ecosystem component which may be adversely affected. Since little information on population numbers and activities in the study area is available, a survey was conducted to determine raptor use of the proposed route to minimize adverse impacts of the development. Field data was the primary information source, although government officials were consulted for additional information and data files were accessed. Raptors were recorded by species and age class, and apparent activity. One field trip, composed of a ground survey and a helicopter survey, was conducted on July 12, 1986. Suggestions are made for mitigation measures related to such negative aspects as electrocution during construction and maintenance activities, harassment and persecution, collisions with the lines, and electric fields and corona.

  3. VT Foote Brook Natural Channel Design Restoration 2003

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The Foote Brook, located in Johnson, Vermont, is known to biologists and anglers as a high quality stream with significant natural reproduction of...

  4. In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)

    Science.gov (United States)

    Ottinger, Christopher A.; Honeyfield, Dale C.; Densmore, Christine L.; Iwanowicz, Luke R.

    2014-01-01

    In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune functions into context with the ratio of in vivo liver thiamine monophosphate (TMP – biologically inactive form) to thiamine pyrophosphate (TPP – biologically active form) proved to be the best indicator of thiamine depletion impacts as determined using regression modeling. These observed relationships indicated differential effects on the immune measures with bactericidal activity exhibiting an inverse relationship with TMP to TPP ratios, Con A stimulated mitogenesis exhibiting a positive relationship with TMP to TPP ratios and PHA-P stimulated mitogenesis exhibiting no significant relationships. In addition, these relationships showed considerable complexity which included the consistent observation of a thiamine-replete subgroup with characteristics similar to those seen in the leukocytes from thiamine-depleted fish. When considered together, our observations indicate that lake trout leukocytes experience cell-type specific impacts as well as an altered physiologic environment when confronted with a thiamine-limited state.

  5. In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush).

    Science.gov (United States)

    Ottinger, Christopher A; Honeyfield, Dale C; Densmore, Christine L; Iwanowicz, Luke R

    2014-05-01

    In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune functions into context with the ratio of in vivo liver thiamine monophosphate (TMP--biologically inactive form) to thiamine pyrophosphate (TPP--biologically active form) proved to be the best indicator of thiamine depletion impacts as determined using regression modeling. These observed relationships indicated differential effects on the immune measures with bactericidal activity exhibiting an inverse relationship with TMP to TPP ratios, Con A stimulated mitogenesis exhibiting a positive relationship with TMP to TPP ratios and PHA-P stimulated mitogenesis exhibiting no significant relationships. In addition, these relationships showed considerable complexity which included the consistent observation of a thiamine-replete subgroup with characteristics similar to those seen in the leukocytes from thiamine-depleted fish. When considered together, our observations indicate that lake trout leukocytes experience cell-type specific impacts as well as an altered physiologic environment when confronted with a thiamine-limited state. Published by Elsevier Ltd.

  6. The Brooks Act, Is it Relevant Today?

    Science.gov (United States)

    1994-06-01

    Publishing Company, 1990. 7. Fla=., Kenneth. Creatina The ComDuter. Washington, D.C.: The Brookings Institution, 1988. 8. Gilchrist, Bruce, & Wessel...Milton R. Reaulation of the Computer Industry. Montvale: AFIPS Press, 1972. 9. Gore, Al. Creatina A Government That Works Better & Cost Less: Renort

  7. Brook Trout Distribution, Pacific Northwest (updated March, 2006)

    Data.gov (United States)

    Pacific States Marine Fisheries Commission — This dataset is a record of fish distribution and activity for BROOK TROUT contained in the StreamNet database. This feature class was created based on linear event...

  8. The role of groundwater in the effect of climatic warming on stream habitat of brook trout

    International Nuclear Information System (INIS)

    Meisner, J.D.

    1990-01-01

    Freshwater fisheries are linked to climate through the variables of water temperature, water quality and water quantity. These three ecosystem linkages provide a basis for assessments of potential impacts of climate change on fisheries resources. A characteristic of fisheries resources, whether it be the size or distribution of fish populations, or a measure of yield, which can be related to climate through one or more of these linkages, is a useful tool with which to forecast the effects of climate change. A stream population of brook trout is a coldwater fisheries resource that is linked to climate by groundwater. Stream dwelling brook trout at low altitudes rely heavily on groundwater discharge in summer to maintain low stream temperature. Groundwater temperature tracks mean annual air temperature due to the insulative effect of the lower troposphere on the surface of the earth. The effect of elevated groundwater temperature on the stream habitat of brook trout was investigated in two brook trout streams north of Toronto, Ontario, with an energy balance stream temperature model, calibrated to both streams to simulate maximum water temperature observed in the brook trout zones. Simulated maximum summer temperatures from the Goddard Institute for Space Studies scenario reduced the brook trout zones by up to 42%. 17 refs., 2 figs

  9. Thermal regimes, nonnative trout, and their influences on native Bull Trout in the Upper Klamath River Basin, Oregon

    Science.gov (United States)

    Benjamin, Joseph R.; Heltzel, Jeannie; Dunham, Jason B.; Heck, Michael; Banish, Nolan P.

    2016-01-01

    The occurrence of fish species may be strongly influenced by a stream’s thermal regime (magnitude, frequency, variation, and timing). For instance, magnitude and frequency provide information about sublethal temperatures, variability in temperature can affect behavioral thermoregulation and bioenergetics, and timing of thermal events may cue life history events, such as spawning and migration. We explored the relationship between thermal regimes and the occurrences of native Bull Trout Salvelinus confluentus and nonnative Brook Trout Salvelinus fontinalis and Brown Trout Salmo trutta across 87 sites in the upper Klamath River basin, Oregon. Our objectives were to associate descriptors of the thermal regime with trout occurrence, predict the probability of Bull Trout occurrence, and estimate upper thermal tolerances of the trout species. We found that each species was associated with a different suite of thermal regime descriptors. Bull Trout were present at sites that were cooler, had fewer high-temperature events, had less variability, and took longer to warm. Brook Trout were also observed at cooler sites with fewer high-temperature events, but the sites were more variable and Brook Trout occurrence was not associated with a timing descriptor. In contrast, Brown Trout were present at sites that were warmer and reached higher temperatures faster, but they were not associated with frequency or variability descriptors. Among the descriptors considered, magnitude (specifically June degree-days) was the most important in predicting the probability of Bull Trout occurrence, and model predictions were strengthened by including Brook Trout occurrence. Last, all three trout species exhibited contrasting patterns of tolerating longer exposures to lower temperatures. Tolerance limits for Bull Trout were lower than those for Brook Trout and Brown Trout, with contrasts especially evident for thermal maxima. Our results confirm the value of exploring a suite of thermal

  10. William Keith Brooks and the naturalist's defense of Darwinism in the late-nineteenth century.

    Science.gov (United States)

    Nash, Richard

    2015-06-01

    William Keith Brooks was an American zoologist at Johns Hopkins University from 1876 until his death in 1908. Over the course of his career, Brooks staunchly defended Darwinism, arguing for the centrality of natural selection in evolutionary theory at a time when alternative theories, such as neo-Lamarckism, grew prominent in American biology. In his book The Law of Heredity (1883), Brooks addressed problems raised by Darwin's theory of pangenesis. In modifying and developing Darwin's pangenesis, Brooks proposed a new theory of heredity that sought to avoid the pitfalls of Darwin's hypothesis. In so doing he strengthened Darwin's theory of natural selection by undermining arguments for the inheritance of acquired characteristics. In later attacks on neo-Lamarckism, Brooks consistently defended Darwin's theory of natural selection on logical grounds, continued to challenge the idea of the inheritance of acquired characteristics, and argued that natural selection best explained a wide range of adaptations. Finally, he critiqued Galton's statistical view of heredity and argued that Galton had resurrected an outmoded typological concept of species, one which Darwin and other naturalists had shown to be incorrect. Brooks's ideas resemble the "biological species concept" of the twentieth century, as developed by evolutionary biologist Ernst Mayr and others. The late-nineteenth century was not a period of total "eclipse" of Darwinism, as biologists and historians have hitherto seen it. Although the "Modern Synthesis" refers to the reconciliation of post-Mendelian genetics with evolution by natural selection, we might adjust our understanding of how the synthesis developed by seeing it as the culmination of a longer discussion that extends back to the late-nineteenth century.

  11. Independent Confirmatory Survey Summary and Results for the Plum Brook Reactor Facility Sandusky OH

    International Nuclear Information System (INIS)

    Bailey, E.N.

    2008-01-01

    In 1941, the War Department acquired approximately 9,000 acres of land near Sandusky, Ohio and constructed a munitions plant. The Plum Brook Ordnance Works Plant produced munitions, such as TNT, until the end of World War II. Following the war, the land remained idle until the National Advisory Committee for Aeronautics (later known as the National Aeronautics and Space Administration or NASA) obtained 500 acres to construct a nuclear research reactor designed to study the effects of radiation on materials used in space flight. The research reactor was put into operation in 1961 and was the first of fifteen test facilities eventually built by NASA at the Plum Brook Station. By 1963, NASA had acquired the remaining land at Plum Brook for these additional test facilities. After successfully completing the objective of landing humans on the Moon and returning them safely to Earth, NASA was faced with budget reductions from Congress in 1973. These budgetary constraints caused NASA to cease operations at several research facilities across the country, including those at Plum Brook Station. The major test facilities at Plum Brook were maintained in a standby mode, capable of being reactivated for future use. The Plum Brook Reactor Facility (PBRF) was shut down January 5, 1973 and all of the nuclear fuel was eventually removed and shipped off site to a U.S. Department of Energy facility in Idaho for disposal or reuse. Decommissioning activities are currently underway at the PBRF (NASA 1999). The objectives of the confirmatory survey activities were to provide independent contractor field data reviews and to generate independent radiological data for use by the Nuclear Regulatory Commission (NRC) in evaluating the adequacy and accuracy of the licensee's procedures and final status survey (FSS) results

  12. Combining Radio and PIT-Telemetry to Study the Large and Fine-Scale Movements of Stocked and Wild Brown Trout (Salmo trutta L.) in a Northeastern Stream, Portugal

    OpenAIRE

    Teixeira, Amílcar A. T.; Cortes, Rui M. V.

    2011-01-01

    Stream-resident salmonid movements have been the subject of numerous studies and their behaviour is relatively well-known (Harcup et al., 1984; Heggenes, 1988). For example, brown trout (Salmo trutta) is described as a sedentary species based on the behaviour displayed, often associated to the strong site attachment to a territory or home range (Bridcut & Giller, 1993; Armstrong & Herbert, 1997). Other salmonids like brook (Salvelinus fontinalis) (Roghair & Dolloff, 2005) and c...

  13. VT Foote Brook Natural Channel Design Restoration 2001-2002

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The Foote Brook, located in Johnson, Vermont, is known to biologists and anglers as a high quality stream with significant natural reproduction of...

  14. Restoration of brook valley meadows in the Netherlands

    NARCIS (Netherlands)

    Grootjans, A.P.; Bakker, J.P.; Jansen, A.J.M.; Kemmers, R.H.

    2002-01-01

    Until recently, restoration measures in Dutch brook valley meadows consisted of re-introducing traditional management techniques, such as mowing without fertilisation and low-intensity grazing. In the Netherlands, additional measures, such as rewetting and sod cutting, are now carried out on a large

  15. Kalispel Resident Fish Project : Annual Report, 1995.

    Energy Technology Data Exchange (ETDEWEB)

    Maroney, Joseph; Donley, Christopher; Scott, Jason; Lockwood, Jr., Neil

    1997-06-01

    In 1995 the Kalispel Natural Resource Department (KNRD) in conjunction with the Washington Department of Fish and Wildlife (WDFW) initiated the implementation of a habitat and population enhancement project for bull trout (Salvelinus confluentus), westslope cutthroat trout (Oncorhynchus clarki lewisi) and largemouth bass (Micropterus salmoides). Habitat and population assessments were conducted in seven tributaries of the Box Canyon reach of the Pend Oreille River. Assessments were used to determine the types and quality of habitat that were limiting to native bull trout and cutthroat trout populations. Assessments were also used to determine the effects of interspecific competition within these streams. A bull trout and brook trout (Salvelinus fontinalis) hybridization assessment was conducted to determine the degree of hybridization between these two species. Analysis of the habitat data indicated high rates of sediment and lack of wintering habitat. The factors that contribute to these conditions have the greatest impact on habitat quality for the tributaries of concern. Population data suggested that brook trout have less stringent habitat requirements; therefore, they have the potential to outcompete the native salmonids in areas of lower quality habitat. No hybrids were found among the samples, which is most likely attributable to the limited number of bull trout. Data collected from these assessments were compiled to develop recommendations for enhancement measures. Recommendations for restoration include riparian planting and fencing, instream structures, as well as, removal of non-native brook trout to reduce interspecific competition with native salmonids in an isolated reach of Cee Cee Ah Creek.

  16. Behavioral evidence for a role of chemoreception during reproduction in lake trout

    Science.gov (United States)

    Buchinger, Tyler J.; Li, Weiming; Johnson, Nicholas

    2015-01-01

    Chemoreception is hypothesized to influence spawning site selection, mate search, and synchronization of gamete release in chars (Salvelinus spp.), but behavioral evidence is generally lacking. Here, we provide a survey of the behavioral responses of reproductive male and female lake trout (Salvelinus namaycush) to natural conspecific chemosensory stimuli. A flow-through laboratory assay with side-by-side artificial spawning reefs was used to evaluate behavioral preferences of spawning-phase males and females for chemosensory stimuli from juveniles and from spawning-phase males and females. Males and females preferred male and juvenile stimuli over no stimuli, but only had weak preferences for female stimuli. Only females had a preference for male over juvenile stimuli when given a direct choice between the two. The unexpected observation of male attraction to male stimuli, even when offered female stimuli, indicates a fundamental difference from the existing models of chemical communication in fishes. We discuss our results from the perspectives of prespawning aggregation, mate evaluation, and spawning synchronization. Identification of specific components of the stimuli will allow confirmation of the function and may have management implications for native and invasive populations of lake trout that are ecologically and economically important.

  17. Lead/tin resonator development at the Stony Brook heavy-ion linac

    International Nuclear Information System (INIS)

    Sikora, J.; Ben-Zvi, I.; Brennan, J.M.; Cole, M.; Noe, J.W.

    1988-01-01

    The Stony Brook Nuclear Structure Laboratory (NSL) has operated a superconducting heavy-ion booster linac since April 1983. The 40 copper split-loop resonators were developed and fabricated at Cal-Tech and plated with lead at Stony Brook. These original lead surfaces have given stable performance for the last 4 years, at an average accelerating gradient of about 2.5 MV/m in the high-β section. The low-β resonators however have never run reliably on-line much better than 2.0 MV/m, due to excessive vibration of their rather soft loop arms in the working accelerator environment. For the last 2-3 years the efforts of the Stony Brook accelerator development group have been focused on (1) a retrofit of the low-beta section of the linac with new QWRs and (2) the further development of plated superconducting surfaces. In particular a Sn/Pb alloy has been shown to give resonator performance at least comparable to that obtained with pure Pb but with a greatly simplified plating technique, as discussed below. Recently a possible heavy-ion injector based on superconducting RF quadrupole (RFQ) structures has also been studied. 13 references, 3 figures, 1 table

  18. Influence of species, size and relative abundance on the outcomes of competitive interactions between brook trout and juvenile coho salmon

    Science.gov (United States)

    Thornton, Emily J; Duda, Jeff; Quinn, Thomas P.

    2016-01-01

    Resource competition between animals is influenced by a number of factors including the species, size and relative abundance of competing individuals. Stream-dwelling animals often experience variably available food resources, and some employ territorial behaviors to increase their access to food. We investigated the factors that affect dominance between resident, non-native brook trout and recolonizing juvenile coho salmon in the Elwha River, WA, USA, to see if brook trout are likely to disrupt coho salmon recolonization via interference competition. During dyadic laboratory feeding trials, we hypothesized that fish size, not species, would determine which individuals consumed the most food items, and that species would have no effect. We found that species, not size, played a significant role in dominance; coho salmon won 95% of trials, even when only 52% the length of their brook trout competitors. As the pairs of competing fish spent more time together during a trial sequence, coho salmon began to consume more food, and brook trout began to lose more, suggesting that the results of early trials influenced fish performance later. In group trials, we hypothesized that group composition and species would not influence fish foraging success. In single species groups, coho salmon consumed more than brook trout, but the ranges overlapped. Brook trout consumption remained constant through all treatments, but coho salmon consumed more food in treatments with fewer coho salmon, suggesting that coho salmon experienced more intra- than inter-specific competition and that brook trout do not pose a substantial challenge. Based on our results, we think it is unlikely that competition from brook trout will disrupt Elwha River recolonization by coho salmon.

  19. Marginal Experiments: Peter Brook and Stepping out Theatre Company

    Science.gov (United States)

    Harpin, Anna

    2010-01-01

    This article juxtaposes the recent work of Peter Brook with a Bristol-based mental health service-user collective--Stepping Out Theatre Company. Informed by field-work with the company, this chapter explores the aesthetic and political relationship between professional, experimental theatre work and community-based performance practice. Drawing…

  20. Robust estimates of environmental effects on population vital rates: an integrated capture–recapture model of seasonal brook trout growth, survival and movement in a stream network

    Science.gov (United States)

    Letcher, Benjamin H.; Schueller, Paul; Bassar, Ronald D.; Nislow, Keith H.; Coombs, Jason A.; Sakrejda, Krzysztof; Morrissey, Michael; Sigourney, Douglas B.; Whiteley, Andrew R.; O'Donnell, Matthew J.; Dubreuil, Todd L.

    2015-01-01

    Modelling the effects of environmental change on populations is a key challenge for ecologists, particularly as the pace of change increases. Currently, modelling efforts are limited by difficulties in establishing robust relationships between environmental drivers and population responses.We developed an integrated capture–recapture state-space model to estimate the effects of two key environmental drivers (stream flow and temperature) on demographic rates (body growth, movement and survival) using a long-term (11 years), high-resolution (individually tagged, sampled seasonally) data set of brook trout (Salvelinus fontinalis) from four sites in a stream network. Our integrated model provides an effective context within which to estimate environmental driver effects because it takes full advantage of data by estimating (latent) state values for missing observations, because it propagates uncertainty among model components and because it accounts for the major demographic rates and interactions that contribute to annual survival.We found that stream flow and temperature had strong effects on brook trout demography. Some effects, such as reduction in survival associated with low stream flow and high temperature during the summer season, were consistent across sites and age classes, suggesting that they may serve as robust indicators of vulnerability to environmental change. Other survival effects varied across ages, sites and seasons, indicating that flow and temperature may not be the primary drivers of survival in those cases. Flow and temperature also affected body growth rates; these responses were consistent across sites but differed dramatically between age classes and seasons. Finally, we found that tributary and mainstem sites responded differently to variation in flow and temperature.Annual survival (combination of survival and body growth across seasons) was insensitive to body growth and was most sensitive to flow (positive) and temperature (negative

  1. Brooke-Spiegler syndrome

    Directory of Open Access Journals (Sweden)

    Layegh Pouran

    2008-01-01

    Full Text Available The Brooke-Spiegler syndrome (BSS is an uncommon autosomal dominant disorder characterized by a high affinity to form multiple adnexal neoplasia (skin appendage tumors, especially trichoepitheliomas and cylindromas, and occasionally spiradenomas, which usually appear in the second or third decade of life. To date, only a few cases with this syndrome have been reported. This case report describes a 26-year-old woman who presented to the dermatology department of Qaem Hospital with tumoral lesions on her scalp, face, and forearm. Her father and younger brother were also affected. On examination, several round-to-oval skin-colored papules with a smooth pearly surface measuring 2 to 6 mm in diameter were seen on the mid-face, particularly in the nasolabial folds, the upper lip. Tumors and nodules seen on the scalp were pinkish red, dome-shaped, and to some extent, pedunculated with surface telangiectasia and induration. Histopathology of the facial papules showed trichoepithelioma while that of a scalp nodule showed cylindroma.

  2. Grandy Brook Station to Hope Brook Station transmission line, environmental impact statement

    Energy Technology Data Exchange (ETDEWEB)

    1987-01-01

    Newfoundland and Labrador Hydro proposes to construct a 138kW transmission line to provide power and energy requirements of the Hope Brook Gold Mine. The proposed route runs approximately 33 km adjacent to Route 480, requiring a cleared right-of-way approximately 30 m wide, with access provided by an all terrain vehicle trail. Concerns were expressed about possible adverse effects on terrestrial and aquatic habitat, water resources, resource utilization, wildlife, and historic resources. This document provides a description of the project and the rationale; the baseline environmental conditions; the environmental protection plan agreed to by Hydro, including mitigation measures and predicted impacts; rehabilitation measures; monitoring; and public involvement. Hydro's studies concluded there would be no significant impact on the environment, including the La Poile caribou herd or the raptors affected. 33 refs., 5 figs., 3 tabs.

  3. Radio-transmitted electromyogram signals as indicators of swimming speed in lake trout and brown trout

    DEFF Research Database (Denmark)

    Thorstad, E.B.; Økland, F.; Koed, Anders

    2000-01-01

    Swimming speed and average electromyogram (EMG) pulse intervals were highly correlated in individual lake trout Salvelinus namaycush (r(2)=0.52-0.89) and brown trout Salmo trutta (r(2)=0.45-0.96). High correlations were found also for pooled data in both lake trout (r(2)=0.90) and brown trout...... of the Ema stock (r(2)=0.96) and Laerdal stock (r(2)=0.96). The linear relationship between swimming speed and average EMG pulse intervals differed significantly among lake trout and the brown trout stocks. This successful calibration of EMGs to swimming speed opens the possibility of recording swimming...... speed of free swimming lake trout and brown trout in situ. EMGs can also be calibrated to oxygen consumption to record energy expenditure. (C) 2000 The Fisheries Society of the British Isles...

  4. Visual observations of historical lake trout spawning grounds in western Lake Huron

    Science.gov (United States)

    Nester, Robert T.; Poe, Thomas P.

    1987-01-01

    Direct underwater video observations were made of the bottom substrates at 12 spawning grounds formerly used by lake trout Salvelinus namaycush in western Lake Huron to evaluate their present suitability for successful reproduction by lake trout. Nine locations examined north of Saginaw Bay in the northwestern end of the lake are thought to provide the best spawning habitat. The substrate at these sites consisted of angular rough cobble and rubble with relatively deep interstitial spaces (a?Y 0.5 m), small amounts of fine sediments, and little or no periphytic growth. Conditions at the three other sampling locations south of Saginaw Bay seemed much less suitable for successful reproduction based on the reduced area of high-quality substrate, shallow interstitial spaces, high infiltration of fine sediments, and greater periphytic growth.

  5. Feasibility of target communities in a Dutch brook valley system

    NARCIS (Netherlands)

    Prins, AH; Bekker, RM

    As a reaction to the ongoing deterioration of nature conservation interest in The Netherlands, an offensive nature strategy was formulated in the 1990 Nature Policy Plan. In this Plan, target communities and target plant species are mentioned. For the 'Drentse A brook valley system', target

  6. Bull trout distribution and abundance in the waters on and bordering the Warm Springs Indian Reservation: 2001 annual report

    International Nuclear Information System (INIS)

    Brun, Christopher V.; Dodson, Rebekah D.

    2002-01-01

    The range of bull trout (Salvelinus confluentus) in the Deschutes River basin has decreased from historic levels due to many factors including dam construction, habitat degradation, brook trout introduction and eradication efforts. While the bull trout population appears to be stable in the Metolius River-Lake Billy Chinook system they have been largely extirpated from the upper Deschutes River (Buchanan et al. 1997). Little was known about bull trout in the lower Deschutes basin until BPA funded project No.9405400 began during 1998. In this progress report we describe the findings from the fourth year (2001) of the multi-year study aimed at determining the life history, habitat needs and limiting factors of bull trout in the lower Deschutes subbasin. Juvenile bull trout and brook trout (Salvelinus fontinalis) relative abundance was assessed in the Warm Springs River and Shitike Creek by night snorkeling. In the Warm Springs R. juvenile bull trout were slightly more numerous than brook trout, however, both were found in low densities. Relative densities of both species were the lowest observed since surveys began in 1999. Relative densities of juvenile bull trout and brook trout increased in Shitike Cr. Juvenile bull trout vastly out numbered brook trout in Shitike Cr. The utility of using index reaches to monitor trends in juvenile bull trout and brook trout relative abundance was assessed in the Warm Springs R. for the third year. Mean relative densities of juvenile bull trout within the index reaches was slightly higher than what was observed in the 2.4 km control reach. However, the mean relative density of brook trout in the 2.4 km control reach was slightly higher than what was observed in within the index reaches. Habitat use by both juvenile bull trout and brook trout was determined in the Warm Springs R. Juvenile bull trout and brook trout occupied pools more frequently than glides, riffles and rapids. However, pools accounted for only a small percentage

  7. Relationships between the Brook Street Terrane and Median Tectonic Zone (Median Batholith) : evidence from Jurassic conglomerates

    International Nuclear Information System (INIS)

    Tulloch, A.J.; Kimbrough, D.L.; Landis, C.A.; Mortimer, N.; Johnston, M.R.

    1999-01-01

    U-Pb zircon ages of 237-180 Ma and c. 280 Ma of seven granitoid clasts from the Rainy River Conglomerate which lies within the eastern Median Tectonic Zone (Median Batholith) in Nelson, and the Barretts Formation of the Brook Street Terrane in Southland, constrain the depositional ages of both units to be no older than c. 180-200 Ma (Early Jurassic). The minimum age of the Rainy River Conglomerate is constrained by the 147 +2 -1 Ma (Latest Jurassic) emplacement age of the One Mile Gabbronorite (new name: previously western Buller Diorite). The ages and chemistry of five of the granitoid clasts are broadly compatible with derivation from rocks that are now represented by Triassic plutons of the Median Tectonic Zone (Median Batholith), although ages as young as 180 Ma are slightly outside the range of the latter as currently exposed in New Zealand. The age (273-290 Ma, 237 +/- 3 Ma) and chemistry of the other two clasts (one each from Rainy River Conglomerate and Barretts Formation) suggest derivation from the Brook Street Terrane. Similarity in stratigraphic age, depositional characteristics, granitoid clast ages and composition between Rainy River Conglomerate and Barretts Formation suggests that they are broadly correlative and collectively overlapped a combined Brook Street Terrane - Median Batholith (MTZ) before the Late Jurassic (147 +2 -1 Ma). Sedimentary overlap may also have continued across to Middle Jurassic conglomeratic strata in the Murihiku Terrane to the east of the Brook Street Terrane. A U-Pb zircon age of 261 +/- 2 Ma is reported for Pourakino Trondhjemite of the Brook Street Terrane. (author). 56 refs., 10 figs., 4 tabs

  8. Unraveling the Timing of Fluid Migration and Trap Formation in the Brooks Range Foothills: A Key to Discovering Hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Catherine L. Hanks

    2008-12-31

    Naturally occurring fractures can play a key role in the evolution and producibility of a hydrocarbon accumulation. Understanding the evolution of fractures in the Brooks Range/Colville basin system of northern Alaska is critical to developing a better working model of the hydrocarbon potential of the region. This study addressed this problem by collecting detailed and regional data on fracture distribution and character, structural geometry, temperature, the timing of deformation along the Brooks Range rangefront and adjacent parts of the Colville basin, and the in situ stress distribution within the Colville basin. This new and existing data then were used to develop a model of how fractures evolved in northern Alaska, both spatially and temporally. The results of the study indicate that fractures formed episodically throughout the evolution of northern Alaska, due to a variety of mechanisms. Four distinct fracture sets were observed. The earliest fractures formed in deep parts of the Colville basin and in the underlying Ellesmerian sequence rocks as these rocks experienced compression associated with the growing Brooks Range fold-and-thrust belt. The orientation of these deep basin fractures was controlled by the maximum in situ horizontal stress in the basin at the time of their formation, which was perpendicular to the active Brooks Range thrust front. This orientation stayed consistently NS-striking for most of the early history of the Brooks Range and Colville basin, but changed to NW-striking with the development of the northeastern Brooks Range during the early Tertiary. Subsequent incorporation of these rocks into the fold-and-thrust belt resulted in overprinting of these deep basin fractures by fractures caused by thrusting and related folding. The youngest fractures developed as rocks were uplifted and exposed. While this general order of fracturing remains consistent across the Brooks Range and adjacent Colville basin, the absolute age at any one

  9. A comparative examination of cortisol effects on muscle myostatin and HSP90 gene expression in salmonids.

    Science.gov (United States)

    Galt, Nicholas J; McCormick, Stephen D; Froehlich, Jacob Michael; Biga, Peggy R

    2016-10-01

    Cortisol, the primary corticosteroid in teleost fishes, is released in response to stressors to elicit local functions, however little is understood regarding muscle-specific responses to cortisol in these fishes. In mammals, glucocorticoids strongly regulate the muscle growth inhibitor, myostatin, via glucocorticoid response elements (GREs) leading to muscle atrophy. Bioinformatics methods suggest that this regulatory mechanism is conserved among vertebrates, however recent evidence suggests some fishes exhibit divergent regulation. Therefore, the aim of this study was to evaluate the conserved actions of cortisol on myostatin and hsp90 expression to determine if variations in cortisol interactions have emerged in salmonid species. Representative salmonids; Chinook salmon (Oncorhynchus tshawytscha), cutthroat trout (Oncorhynchus clarki), brook trout (Salvelinus fontinalis), and Atlantic salmon (Salmo salar); were injected intraperitoneally with a cortisol implant (50μg/g body weight) and muscle gene expression was quantified after 48h. Plasma glucose and cortisol levels were significantly elevated by cortisol in all species, demonstrating physiological effectiveness of the treatment. HSP90 mRNA levels were elevated by cortisol in brook trout, Chinook salmon, and Atlantic salmon, but were decreased in cutthroat trout. Myostatin mRNA levels were affected in a species, tissue (muscle type), and paralog specific manner. Cortisol treatment increased myostatin expression in brook trout (Salvelinus) and Atlantic salmon (Salmo), but not in Chinook salmon (Oncorhynchus) or cutthroat trout (Oncorhynchus). Interestingly, the VC alone increased myostatin mRNA expression in Chinook and Atlantic salmon, while the addition of cortisol blocked the response. Taken together, these results suggest that cortisol affects muscle-specific gene expression in species-specific manners, with unique Oncorhynchus-specific divergence observed, that are not predictive solely based upon

  10. NASA's Hydrogen Outpost: The Rocket Systems Area at Plum Brook Station

    Science.gov (United States)

    Arrighi, Robert S.

    2016-01-01

    "There was pretty much a general knowledge about hydrogen and its capabilities," recalled former researcher Robert Graham. "The question was, could you use it in a rocket engine? Do we have the technology to handle it? How will it cool? Will it produce so much heat release that we can't cool the engine? These were the questions that we had to address." The National Aeronautics and Space Administration's (NASA) Glenn Research Center, referred to historically as the Lewis Research Center, made a concerted effort to answer these and related questions in the 1950s and 1960s. The center played a critical role transforming hydrogen's theoretical potential into a flight-ready propellant. Since then NASA has utilized liquid hydrogen to send humans and robots to the Moon, propel dozens of spacecraft across the universe, orbit scores of satellite systems, and power 135 space shuttle flights. Rocket pioneers had recognized hydrogen's potential early on, but its extremely low boiling temperature and low density made it impracticable as a fuel. The Lewis laboratory first demonstrated that liquid hydrogen could be safely utilized in rocket and aircraft propulsion systems, then perfected techniques to store, pump, and cleanly burn the fuel, as well as use it to cool the engine. The Rocket Systems Area at Lewis's remote testing area, Plum Brook Station, played a little known, but important role in the center's hydrogen research efforts. This publication focuses on the activities at the Rocket Systems Area, but it also discusses hydrogen's role in NASA's space program and Lewis's overall hydrogen work. The Rocket Systems Area included nine physically modest test sites and three test stands dedicated to liquid-hydrogen-related research. In 1962 Cleveland Plain Dealer reporter Karl Abram claimed, "The rocket facility looks more like a petroleum refinery. Its test rigs sprout pipes, valves and tanks. During the night test runs, excess hydrogen is burned from special stacks in the best

  11. Quantitative Analysis of Contributing Factors Affecting Patient Satisfaction in Family Medicine Service Clinics at Brooke Army Medical Center

    Science.gov (United States)

    2008-06-06

    Predictors of patient satisfaction for Brooke Army Medical Center Family Medicine Service primary care clinics was performed. Data was obtained from...Factors Affecting Patient Satisfaction in Family Medicine Service Clinics at Brooke Army Medical Center Presented to MAJ Eric Schmacker, Ph.D. In...study. All patients ’ medical information was protected at all times and under no circumstances will be discussed or released to any outside agency

  12. Fish resource data from the Snare River, Northwest Territories

    International Nuclear Information System (INIS)

    Jessop, E.F.; Chang-Kue, K.T.J.; MacDonald, G.

    1994-01-01

    An extensive fish sampling and tagging program was conducted on the Snare River, Northwest Territories, in order to collect baseline data on the fish populations in sections of the river altered by hydroelectric projects. Fish populations were sampled from May to July 1977 in five sections of the river that were influenced by development of hydropower at three dams currently on line; 530 tagged fish were also released. The biweekly catch composition in experimental gill nets for each study area and the catch per gill net mesh size are presented for walleye (Stizostedion vitreum), lake trout (Salvelinus namaycush), lake whitefish (Coregonus clupeaformis), lake cisco (Coregonus artedi), northern pike (Esox lucius), white sucker (Catostomus commersoni), and longnose sucker (Catostomus catostomus). Age-specific data on length, weight, age, sex, and maturity are also included. 7 refs., 12 figs., 42 tabs

  13. Olemuse teater. Kantor ja Brook / Jan Kott ; tõlk. Eva-Liisa Linder

    Index Scriptorium Estoniae

    Kott, Jan

    2004-01-01

    T. Kantori lavastustest "Surnud klass" ja "Wielopole Wielopole" ning P. Brooki "Carmeni" lavastusest. Tõlgitud raamatust : Jan Kott. The Theatre of Essence: Kantor and Brook.- The Theatre of Essence and other Essays. Evanston, Northwestern University Press, 1984, lk. 159-165

  14. The evolution of the science of Bear Brook Watershed in Maine, USA

    Czech Academy of Sciences Publication Activity Database

    Norton, S. A.; Fernandez, I. J.; Kahl, J. S.; Rustad, L. E.; Navrátil, Tomáš; Almquist, H.

    2010-01-01

    Roč. 171, 1/4 (2010), s. 3-21 ISSN 0167-6369 Institutional research plan: CEZ:AV0Z30130516 Keywords : Bear Brook Watershed in Maine * acidification * stream * soil * nitrogen * sulfur * phosphorus * base cations Subject RIV: DD - Geochemistry Impact factor: 1.436, year: 2010

  15. Stony Brook's Graduate Courses in Clear, Vivid, Conversational Communication

    Science.gov (United States)

    Bass, E.

    2011-12-01

    Graduate students in the sciences at Stony Brook University are taking for-credit courses to learn to communicate more effectively about science with people outside their disciplines, including public officials, the press, students, potential funders and employers, colleagues in other fields, and the general public. Five Communicating Science courses are offered; two more will be added in January, 2012. The courses are offered by the School of Journalism and developed by the Center for Communicating Science (CCS). This interdisciplinary center was founded in 2009, with the participation of Alan Alda, the actor, writer, director and longtime advocate for science, who is a Visiting Professor at Stony Brook. At the core of the program are three 1-credit (14-hour) modules that rely on experiential learning, repeated practice and immediate, interactive feedback. In Distilling Your Message, students practice speaking clearly, vividly and conversationally about their work at different levels of complexity and formality to different audiences, using storytelling techniques where appropriate. In Writing for the Public, they extend these skills into writing. In Improvisation for Scientists, the most unconventional of the courses, students play improvisational theater games to help themselves connect more directly, personally and responsively with their audiences. In their first two semesters, the courses are expected to serve about 90 students, taking a total of about 180 credits. Most of the courses have filled quickly, mixing master's and doctoral students from more than a dozen fields, including marine and atmospheric sciences. Three to six credits of Communicating Science courses are required for students in two programs, an MA in Marine Conservation and Policy and an Advanced Certificate in Health Communications. The content and methods of the courses are based largely on lessons learned from evaluations of all-day workshops that CCS has conducted for more than 250

  16. Chemical and morphological distinctions between vertical and lateral podzolization at Hubbard Brook

    Science.gov (United States)

    Rebecca R. Bourgault; Donald S. Ross; Scott W. Bailey

    2015-01-01

    Classical podzolization studies assumed vertical percolation and pedon-scale horizon development. However, hillslope-scale lateral podzolization also occurs where lateral subsurface water flux predominates. In this hydropedologic study, 99 podzols were observed in Watershed 3, Hubbard Brook Experimental Forest, New Hampshire. Soil horizon samples were extracted with...

  17. Can nutrient limitations explain low and declining white spruce growth near the Arctic treeline in the eastern Brooks Range, Alaska?

    Science.gov (United States)

    Ellison, S.; Sullivan, P. F.

    2014-12-01

    The position of the Arctic treeline is of critical importance for global carbon cycling and surface energy budgets. However, controls on tree growth at treeline remain uncertain. In the Alaskan Brooks Range, 20th century warming has caused varying growth responses among treeline trees, with trees in the west responding positively, while trees in the east have responded negatively. The prevailing explanation of this trend ascribes the negative growth response to warming-induced drought stress in the eastern Brooks Range. However, recent measurements of carbon isotope discrimination in tree rings, xylem sap flow and needle gas exchange suggest that drought stress cannot explain these regional growth declines. Additionally, evidence from the western Brooks Range suggests that nutrient availability, rather than drought stress, may be the proximate control on tree growth. In this study, we investigated the hypothesis that low and declining growth of eastern Brooks Range trees is due to low and declining soil nutrient availability, which may continue to decrease with climate change as soils become drier and microbial activity declines. We compared microclimate, tree performance, and a wide range of proxies for soil nutrient availability in four watersheds along a west-east transect in the Brooks Range during the growing seasons of 2013 and 2014. We hypothesized that soil nutrient availability would track closely with the strong west-east precipitation gradient, with higher rainfall and greater soil nutrient availability in the western Brooks Range. We expected to find that soil water contents in the west are near optimum for nitrogen mineralization, while those in the east are below optimum. Needle nitrogen concentration, net photosynthesis, branch extension growth, and growth in the main stem are expected to decline with the hypothesized decrease in soil nutrient availability. The results of our study will elucidate the current controls on growth of trees near the

  18. Toxicity of fluoride to aquatic species and evaluation of toxicity modifying factors.

    Science.gov (United States)

    Pearcy, Krysta; Elphick, James; Burnett-Seidel, Charlene

    2015-07-01

    The present study was performed to investigate the toxicity of fluoride to a variety of freshwater aquatic organisms and to establish whether water quality variables contribute substantively to modifying its toxicity. Water hardness, chloride, and alkalinity were tested as possible toxicity modifying factors for fluoride using acute toxicity tests with Hyalella azteca and Oncorhynchus mykiss. Chloride appeared to be the major toxicity modifying factor for fluoride in these acute toxicity tests. The chronic toxicity of fluoride was evaluated with a variety of species, including 3 fish (Pimephales promelas, O. mykiss, and Salvelinus namaycush), 3 invertebrates (Ceriodaphnia dubia, H. azteca, and Chironomus dilutus), 1 plant (Lemna minor), and 1 alga (Pseudokirchneriella subcapitata). Hyalella azteca was the most sensitive species overall, and O. mykiss was the most sensitive species of fish. The role of chloride as a toxicity modifying factor was inconsistent between species in the chronic toxicity tests. © 2015 SETAC.

  19. New records of Ergasilus (Copepoda: Ergasilidae) in the Laurentian Great Lakes, including a lakewide review of records and host associations

    Science.gov (United States)

    Hudson, Patrick L.; Bowen, Charles A.; Stedman, Ralph M.

    1994-01-01

    Ergasilus nerkae was found infecting ninespine stickleback (Pungitius pungitius) in lakes Huron, Michigan, and Superior and threespine stickleback (Gasterosteus aculeatus) and round whitefish (Prosopium cylindraceum) in Lake Huron. Based upon the literature and study of archived material, we propose that E. nerkae is enzootic to the Great Lakes and that ninespine stickleback are a preferred host in Lake Huron. Prevalence of E. nerkae on ninespine stickleback increased from 17% in June to 68% in September, but mean intensity remained light. Prevalence and mean intensity increased with host length. Ergasilus luciopercarum is also reported on lake trout (Salvelinus namaycush) and largemouth bass (Micropterus salmoides) for the first time. Host-parasite records of Ergasilus spp. in North America are reviewed, biology and taxonomy are summarized, and a checklist of Great Lakes host-parasite-locality records is provided. At present, eight species of Ergasilus are known to infect 42 Great Lakes fish species.

  20. Long-term trends from ecosystem research at the Hubbard Brook Experimental Forest

    Science.gov (United States)

    John L. Campbell; Charles T. Driscoll; Christopher Eagar; Gene E. Likens; Thomas G. Siccama; Chris E. Johnson; Timothy J. Fahey; Steven P. Hamburg; Richard T. Holmes; Amey S. Bailey; Donald C. Buso

    2007-01-01

    Summarizes 52 years of collaborative, long-term research conducted at the Hubbard Brook (NH) Experimental Forest on ecosystem response to disturbances such as air pollution, climate change, forest disturbance, and forest management practices. Also provides explanations of some of the trends and lists references from scientific literature for further reading.

  1. Species replacement by a nonnative salmonid alters ecosystem function by reducing prey subsidies that support riparian spiders

    Science.gov (United States)

    Benjamin, J.R.; Fausch, K.D.; Baxter, C.V.

    2011-01-01

    Replacement of a native species by a nonnative can have strong effects on ecosystem function, such as altering nutrient cycling or disturbance frequency. Replacements may cause shifts in ecosystem function because nonnatives establish at different biomass, or because they differ from native species in traits like foraging behavior. However, no studies have compared effects of wholesale replacement of a native by a nonnative species on subsidies that support consumers in adjacent habitats, nor quantified the magnitude of these effects. We examined whether streams invaded by nonnative brook trout (Salvelinus fontinalis) in two regions of the Rocky Mountains, USA, produced fewer emerging adult aquatic insects compared to paired streams with native cutthroat trout (Oncorhynchus clarkii), and whether riparian spiders that depend on these prey were less abundant along streams with lower total insect emergence. As predicted, emergence density was 36% lower from streams with the nonnative fish. Biomass of brook trout was higher than the cutthroat trout they replaced, but even after accounting for this difference, emergence was 24% lower from brook trout streams. More riparian spiders were counted along streams with greater total emergence across the water surface. Based on these results, we predicted that brook trout replacement would result in 6-20% fewer spiders in the two regions. When brook trout replace cutthroat trout, they reduce cross-habitat resource subsidies and alter ecosystem function in stream-riparian food webs, not only owing to increased biomass but also because traits apparently differ from native cutthroat trout. ?? 2011 Springer-Verlag.

  2. The Missing Main Effect of Welfare State Regimes: A Replication of ‘Social Policy Responsiveness in Developed Democracies’ by Brooks and Manza

    Directory of Open Access Journals (Sweden)

    Nate Breznau

    2015-08-01

    Full Text Available This article reports the results of a replication of Brooks and Manza's "Social Policy Responsiveness in Developed Democracies" published in 2006 in the American Sociological Review. The article finds that Brooks and Manza utilized an interaction term but excluded the main effect of one of the interacted variables. This model specification has specific implications: statistically, that the omitted main effect variable has no correlation with the residual error term from their regression; theoretically speaking, this means that all unobserved historical, cultural, and other characteristics that distinguish liberal democratic welfare regimes from others can be accounted for with a handful of quantitative measures. Using replicated data, this article finds that the Brooks and Manza models fail these assumptions. A sensitivity analysis using more than 800 regressions with different configurations of variables confirms this. In 99.5 percent of the cases, addition of the main effect removes Brooks and Manza's empirical findings completely. A theoretical discussion illuminates why these findings are not surprising. This article provides a reminder that models and theories are coterminous, each implied by the other.

  3. Kalispel Non-Native Fish Suppression Project 2007 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Wingert, Michele; Andersen, Todd [Kalispel Natural Resource Department

    2008-11-18

    Non-native salmonids are impacting native salmonid populations throughout the Pend Oreille Subbasin. Competition, hybridization, and predation by non-native fish have been identified as primary factors in the decline of some native bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarki lewisi) populations. In 2007, the Kalispel Natural Resource Department (KNRD) initiated the Kalispel Nonnative Fish Suppression Project. The goal of this project is to implement actions to suppress or eradicate non-native fish in areas where native populations are declining or have been extirpated. These projects have previously been identified as critical to recovering native bull trout and westslope cutthroat trout (WCT). Lower Graham Creek was invaded by non-native rainbow (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis) after a small dam failed in 1991. By 2003, no genetically pure WCT remained in the lower 700 m of Graham Creek. Further invasion upstream is currently precluded by a relatively short section of steep, cascade-pool stepped channel section that will likely be breached in the near future. In 2008, a fish management structure (barrier) was constructed at the mouth of Graham Creek to preclude further invasion of non-native fish into Graham Creek. The construction of the barrier was preceded by intensive electrofishing in the lower 700 m to remove and relocate all captured fish. Westslope cutthroat trout have recently been extirpated in Cee Cee Ah Creek due to displacement by brook trout. We propose treating Cee Cee Ah Creek with a piscicide to eradicate brook trout. Once eradication is complete, cutthroat trout will be translocated from nearby watersheds. In 2004, the Washington Department of Fish and Wildlife (WDFW) proposed an antimycin treatment within the subbasin; the project encountered significant public opposition and was eventually abandoned. However, over the course of planning this 2004 project, little public

  4. Stream pH as an abiotic gradient influencing distributions of trout in Pennsylvania streams

    Science.gov (United States)

    Kocovsky, P.M.; Carline, R.F.

    2005-01-01

    Elevation and stream slope are abiotic gradients that limit upstream distributions of brook trout Salvelinus fontinalis and brown trout Salmo trutta in streams. We sought to determine whether another abiotic gradient, base-flow pH, may also affect distributions of these two species in eastern North America streams. We used historical data from the Pennsylvania Fish and Boat Commission's fisheries management database to explore the effects of reach elevation, slope, and base-flow pH on distributional limits to brook trout and brown trout in Pennsylvania streams in the Appalachian Plateaus and Ridge and Valley physiographic provinces. Discriminant function analysis (DFA) was used to calculate a canonical axis that separated allopatric brook trout populations from allopatric brown trout populations and allowed us to assess which of the three independent variables were important gradients along which communities graded from allopatric brook trout to allopatric brown trout. Canonical structure coefficients from DFA indicated that in both physiographic provinces, stream base-flow pH and slope were important factors in distributional limits; elevation was also an important factor in the Ridge and Valley Province but not the Appalachian Plateaus Province. Graphs of each variable against the proportion of brook trout in a community also identified apparent zones of allopatry for both species on the basis of pH and stream slope. We hypothesize that pH-mediated interspecific competition that favors brook trout in competition with brown trout at lower pH is the most plausible mechanism for segregation of these two species along pH gradients. Our discovery that trout distributions in Pennsylvania are related to stream base-flow pH has important implications for brook trout conservation in acidified regions. Carefully designed laboratory and field studies will be required to test our hypothesis and elucidate the mechanisms responsible for the partitioning of brook trout and

  5. A Study to Evaluate the Organization and the Operating Procedures of the Patient Assistance Function at Brooke Army Medical Center, Fort Sam Houston, Texas

    Science.gov (United States)

    1979-08-01

    15 March 1979. 59Interview with Wendy L. Farace , Head Nurse, Obstetrics/Gynecology Clinic, Brooke Army Medical Center, Fort Sam Houston, Texas, 8...6 February 1979. Farace , Wendy L. Head Nurse, Obstetrica/Gynecology Clinic, Brooke Army Medical Center, Fort Sam Houston, Texas. Interview, 8 January

  6. Temperature, pressure and light data collected by attached Archival Transmitting Tags to Salvelinus malma (Dolly Varden trout) in the Wulik River, Alaska, during 2012-06 to 2013-10 (NODC Accession 0119954)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains data collected by electronic tags (Pop-up Satellite Archival Transmitting) attached to Salvelinus malma (Dolly Varden trout) in the Wulik...

  7. Do low-mercury terrestrial resources subsidize low-mercury growth of stream fish? Differences between species along a productivity gradient.

    Directory of Open Access Journals (Sweden)

    Darren M Ward

    Full Text Available Low productivity in aquatic ecosystems is associated with reduced individual growth of fish and increased concentrations of methylmercury (MeHg in fish and their prey. However, many stream-dwelling fish species can use terrestrially-derived food resources, potentially subsidizing growth at low-productivity sites, and, because terrestrial resources have lower MeHg concentrations than aquatic resources, preventing an increase in diet-borne MeHg accumulation. We used a large-scale field study to evaluate relationships among terrestrial subsidy use, growth, and MeHg concentrations in two stream-dwelling fish species across an in-stream productivity gradient. We sampled young-of-the-year brook trout (Salvelinus fontinalis and Atlantic salmon (Salmo salar, potential competitors with similar foraging habits, from 20 study sites in streams in New Hampshire and Massachusetts that encompassed a wide range of aquatic prey biomass. Stable isotope analysis showed that brook trout used more terrestrial resources than Atlantic salmon. Over their first growing season, Atlantic salmon tended to grow larger than brook trout at sites with high aquatic prey biomass, but brook grew two-fold larger than Atlantic salmon at sites with low aquatic prey biomass. The MeHg concentrations of brook trout and Atlantic salmon were similar at sites with high aquatic prey biomass and the MeHg concentrations of both species increased at sites with low prey biomass and high MeHg in aquatic prey. However, brook trout had three-fold lower MeHg concentrations than Atlantic salmon at low-productivity, high-MeHg sites. These results suggest that differential use of terrestrial resource subsidies reversed the growth asymmetry between potential competitors across a productivity gradient and, for one species, moderated the effect of low in-stream productivity on MeHg accumulation.

  8. Kitchenette: Hell or Home? Different"Kitchenette"Images Created by Richard Wright and Gwendolyn Brooks%Kitchenette:Hell or Home?Different"Kitchenette"Images Created by Richard Wright and Gwendolyn Brooks

    Institute of Scientific and Technical Information of China (English)

    肖志宏

    2017-01-01

    How the southern black migrants navigate and survive the northern urban space is a question profoundly explored and realistically documented in many migration narratives in African American literature. This paper concentrates on the image of Chicago"kitchenette"in the works of Richard Wright and Gwendolyn Brooks, in an attempt to demonstrate the different inter-pretations of migrant spaces on the urban landscape.

  9. EXPERIMENTAL ACIDIFICATION CAUSES SOIL BASE-CATION DEPLETION AT THE BEAR BROOK WATERSHED IN MAINE

    Science.gov (United States)

    There is concern that changes in atmospheric deposition, climate, or land use have altered the biogeochemistry of forests causing soil base-cation depletion, particularly Ca. The Bear Brook Watershed in Maine (BBWM) is a paired watershed experiment with one watershed subjected to...

  10. Assessment of Native Salmonids Above Hells Canyon Dam, Idaho, 2004-2005 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Kevin A.; Lamansky, Jr., James A. (Idaho Department of Fish and Game, Boise, ID)

    2005-08-01

    In the western United States, exotic brook trout Salvelinus fontinalis frequently have a deleterious effect on native salmonids, and biologists often attempt to remove brook trout in streams using electrofishing. Although the success of electrofishing removal projects typically is low, few studies have assessed the underlying mechanisms of failure, especially in terms of compensatory responses. We evaluated the effectiveness of a three-year removal project in reducing brook trout and enhancing native salmonids in 7.8 km of an Idaho stream and looked for brook trout compensatory responses such as decreased natural mortality, increased growth, increased fecundity at length, or earlier maturation. Due to underestimates of the distribution of brook trout in the first year and personnel shortages in the third year, the multiagency watershed advisory group that performed the project fully treated the stream (i.e. multipass removals over the entire stream) in only one year. In 1998, 1999, and 2000, a total of 1,401, 1,241, and 890 brook trout were removed, respectively. For 1999 and 2000, an estimated 88 and 79% of the total number of brook trout in the stream were removed. For the section of stream that was treated in all years, the abundance of age-1 and older brook trout decreased by 85% from 1998 to 2003. In the same area, the abundance of age-0 brook trout decreased 86% from 1998 to 1999 but by 2003 had rebounded to near the original abundance. Abundance of native redband trout Oncorhynchus mykiss decreased for age-1 and older fish but did not change significantly for age-0 fish. Despite high rates of removal, total annual survival rate for brook trout increased from 0.08 {+-} 0.02 in 1998 to 0.20 {+-} 0.04 in 1999 and 0.21 {+-} 0.04 in 2000. Growth of age-0 brook trout was significantly higher in 2000 (the year after their abundance was lowest) compared to other years, and growth of age-1 and age-2 brook trout was significantly lower following the initial removal

  11. Field performance of timber bridges. 10, Sanborn Brook stress-laminated deck bridge

    Science.gov (United States)

    P. D. Hilbrich Lee; J. P. Wacker; M. A. Ritter

    The Sanborn Brook bridge was constructed in August 1991, 10 miles northeast of Concord, New Hampshire, as part of the demonstration timber bridge program of the USDA Forest Service. The bridge is a simple-span, double-lane, stress-laminated deck superstructure constructed from Southern Pine lumber and is approximately 25 ft long and 28 ft wide with a skew of 14 degrees...

  12. Dwarf char, a new form of chars (the genus Salvelinus) in Lake Kronotskoe

    Science.gov (United States)

    Pavlov, S.D.; Pivovarov, E.A.; Ostberg, C.O.

    2012-01-01

    Lake Kronotskoe is situated in the Kronotskii State Nature Reserve and is a unique natural heritage of Kamchatka. The lake–river system of the reserve includes numerous springs and small streams and three large inflowing rivers, Listvennichnaya, Unana, and Uzon, which form the main bays of Lake Kronotskoe; one river (Kronotskaya) flows from the lake. This river is characterized by several rapids, which are assumed to be unsurmountable barriers for fish migration. The ichthyofauna of the lake has been isolated for a long time, and some endemic fishes appeared, including char of the genus Salvelinus and the residential form of red salmon Oncorhynchus nerka (the local name is kokanee). These species are perfect model objects to study microevolution processes. Char of Lake Kronotskoe are characterized by significant polymorphism and plasticity [1–3]; therefore, they are extremely valuable for studying the processes of speciation and form development. That is why the populations of char in Lake Kronotskoe are unique and attract special attention of researchers. 

  13. Effects of dressing and cooking on DDT concentrations in certain fish from Lake Michigan

    Science.gov (United States)

    Reinert, Robert E.; Stewart, Donald; Seagran, Harry L.

    1972-01-01

    Concentrations of DDT residues were highest in parts of the body with the highest oil content in four species of fish from Lake Michigan: yellow perch (Perca flavescens), bloater (Coregonus hoyi), lake trout (Salvelinus namaycush), and coho salmon (Oncorhynchus kisutch). Dressing reduced the DDT residues and oil content by more than 90% in yellow perch but had little effect in the other three species. The concentration of DDT residues in bloaters was changed little by smoking but was reduced 64–72% by other methods of cooking: from 8.0 ppm (raw) to 2.2 ppm after frying in corn oil; from 10.7 to 3.9 ppm after frying in lard; and from 9.1 to 3.2 ppm after broiling. The concentration of DDT residues in fillets of yellow perch changed only from 0.3 ppm (raw) to 0.4 or 0.5 ppm after baking, frying, or broiling.

  14. Lake trout otolith chronologies as multidecadal indicators of high-latitude freshwater ecosystems

    Science.gov (United States)

    Black, B.A.; Von Biela, V.R.; Zimmerman, C.E.; Brown, Randy J.

    2013-01-01

    High-latitude ecosystems are among the most vulnerable to long-term climate change, yet continuous, multidecadal indicators by which to gauge effects on biology are scarce, especially in freshwater environments. To address this issue, dendrochronology (tree-ring analysis) techniques were applied to growth-increment widths in otoliths from lake trout (Salvelinus namaycush) from the Chandler Lake system, Alaska (68.23°N, 152.70°W). All otoliths were collected in 1987 and exhibited highly synchronous patterns in growth-increment width. Increments were dated, the widths were measured, and age-related growth declines were removed using standard dendrochronology techniques. The detrended time series were averaged to generate an annually resolved chronology, which continuously spanned 1964–1984. The chronology positively and linearly correlated with August air temperature over the 22-year interval (p otolith chronologies could be used to examine responses between freshwater ecosystems and environmental variability across a range of temporal and spatial scales.

  15. Nitrile anion cyclization with epoxysilanes followed by Brook rearrangement/ring-opening of cyclopropane nitriles/alkylation.

    Science.gov (United States)

    Okugawa, Seigo; Masu, Hyuma; Yamaguchi, Kentaro; Takeda, Kei

    2005-12-09

    [reactions: see text] The reaction of delta-silyl-gamma,delta-epoxypentanenitrile derivatives 9-12 with a base and an alkylating agent affords (Z)-delta-siloxy-gamma,delta-unsaturated pentanenitrile derivatives via a tandem process that involves the formation of a cyclopropane derivative by epoxy nitrile cyclization followed by Brook rearrangement and an anion-induced cleavage of the cyclopropane ring. Exclusive formation of a (Z)-derivative from trans-epoxides is explained by the reaction pathway that involves a backside displacement of the epoxide by the alpha-nitrile carbanion and the O-Si bond formation followed by concerted processes involving Brook rearrangement and the anti-mode of eliminative ring fission of the cyclopropane from the rotamer 19. The fact that (E)-isomers are exclusively obtained from cis-epoxides and alpha-cyclopropyl-alpha-silylcarbinol derivative 26 provides experimental support for the proposed pathway.

  16. IMPACTS OF MARINE AEROSOLS ON SURFACE WATER CHEMISTRY AT BEAR BROOK WATERSHED, MAINE USA

    Science.gov (United States)

    The East Bear catchment at Bear Brook Watershed, Maine receives moderate (for the eastern U.S.) amounts of Cl- in wet and dry deposition. In 1989, Cl- in precipitation ranged from 2 to 55 eq/L. Dry, occult, and wet deposition plus evapotranspiration resulted in stream Cl- averagi...

  17. A useful single-solution polychrome stain for plant material...Brook Cyte-Chrome I.

    Science.gov (United States)

    Stanley L Krugman; Julia F. Littlefield

    1968-01-01

    Fresh and chemically fixed sectioned plant material can be quickly stained by applying a Brook Cyte Chrome I polychrome stain. Staining time averaged only about 10 minutes. And exact timing of staining and de-staining is not as critical as with most of the commonly used stains. The overall quality is comparable to that of the traditional stains.

  18. SOIL ALUMINUM DISTRIBUTION IN THE NEAR-STREAM ZONE AT THE BEAR BROOK WATERSHED IN MAINE

    Science.gov (United States)

    Near-stream and upslope soil chemical properties were analyzed to infer linkages between soil and surface water chemistry at the Bear Brook Watershed in Maine [BBWM]. Organic and mineral soil samples were collected along six 20 m transects perpendicular to the stream and one 200 ...

  19. Assessing the impact of stocking northern-origin hatchery brook trout on the genetics of wild populations in North Carolina

    Science.gov (United States)

    Kazyak, David C.; Rash, Jacob; Lubinski, Barbara A.; King, Tim L.

    2018-01-01

    The release of hatchery-origin fish into streams with endemics can degrade the genetics of wild populations if interbreeding occurs. Starting in the 1800s, brook trout descendent from wild populations in the northeastern United States were stocked from hatcheries into streams across broad areas of North America to create and enhance fishery resources. Across the southeastern United States, many millions of hatchery-origin brook trout have been released into hundreds of streams, but the extent of introgression with native populations is not well resolved despite large phylogeographic distances between these groups. We used three assessment approaches based on 12 microsatellite loci to examine the extent of hatchery introgression in 406 wild brook trout populations in North Carolina. We found high levels of differentiation among most collections (mean F′ST = 0.718), and among most wild collections and hatchery strains (mean F′ST = 0.732). Our assessment of hatchery introgression was consistent across the three metrics, and indicated that most wild populations have not been strongly influenced by supplemental stocking. However, a small proportion of wild populations in North Carolina appear to have been strongly influenced by stocked conspecifics, or in some cases, may have been founded entirely by hatchery lineages. In addition, we found significant differences in the apparent extent of hatchery introgression among major watersheds, with the Savannah River being the most strongly impacted. Conversely, populations in the Pee Dee River watershed showed little to no evidence of hatchery introgression. Our study represents the first large-scale effort to quantify the extent of hatchery introgression across brook trout populations in the southern Appalachians using highly polymorphic microsatellite markers.

  20. William Graham Brooke (1835-1907): Advocate of Girls' Superior Schooling in Nineteenth-Century Ireland

    Science.gov (United States)

    McCormack, Christopher F.

    2015-01-01

    This paper examines the role of William Graham Brooke as advocate of women's higher education and access to university. His work as advocate is considered against the religious, political, social and economic backdrop of late nineteenth century Ireland. A barrister, as Clerk in the Lord Chancellor's office, he was centrally involved in the…

  1. 2006 Reson 8101ER Multibeam Sonar Data from Cruise AHI-06-12 - Brooks Bank, Northwestern Hawaiian Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Reson 8101ER multibeam Data were collected between 13-15 October 2006 aboard NOAA Survey Launch Acoustic Habitat Investigator (AHI) Brooks Banks in the Northwestern...

  2. Decadal responses in soil N dynamics at the Bear Brook Watershed in Maine, USA

    Science.gov (United States)

    Sultana Jefts; Ivan J. Fernandez; Lindwey E. Rustad; D. Bryan Dail

    2004-01-01

    Atmospheric nitrogen deposition to forested ecosystems is a concern because of both geochemical and biological consequences for ecosystem integrity. High levels of prolonged N deposition can lead to "N saturation" of the ecosystem. The Bear Brook Watershed in Maine is a long-term, paired forested watershed experiment with over a decade of experimental N...

  3. CRED 20 m Gridded bathymetry of Brooks Banks and St. Rogatien Bank, Hawaii, USA (Arc ASCII format)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Gridded bathymetry (20m) of the shelf and slope environments of Brooks Banks and St. Rogatien, Hawaii, USA. The ASCII includes multibeam bathymetry from the Simrad...

  4. Experimental Acidification Causes Soil Base-Cation Depletion at the Bear Brook Watershed in Maine

    Science.gov (United States)

    Ivan J. Fernandez; Lindsey E. Rustad; Stephen A. Norton; Jeffrey S. Kahl; Bernard J. Cosby

    2003-01-01

    There is concern that changes in atmospheric deposition, climate, or land use have altered the biogeochemistry of forests causing soil base-cation depletion, particularly Ca. The Bear Brook Watershed in Maine (BBWM) is a paired watershed experiment with one watershed subjected to elevated N and S deposition through bimonthly additions of (NH4)2SO4. Quantitative soil...

  5. Brown trout and food web interactions in a Minnesota stream

    Science.gov (United States)

    Zimmerman, J.K.H.; Vondracek, B.

    2007-01-01

    1. We examined indirect, community-level interactions in a stream that contained non-native brown trout (Salmo trutta Linnaeus), native brook trout (Salvelinus fontinalis Mitchill) and native slimy sculpin (Cottus cognatus Richardson). Our objectives were to examine benthic invertebrate composition and prey selection of fishes (measured by total invertebrate dry mass, dry mass of individual invertebrate taxa and relative proportion of invertebrate taxa in the benthos and diet) among treatments (no fish, juvenile brook trout alone, juvenile brown trout alone, sculpin with brook trout and sculpin with brown trout). 2. We assigned treatments to 1 m2 enclosures/exclosures placed in riffles in Valley Creek, Minnesota, and conducted six experimental trials. We used three designs of fish densities (addition of trout to a constant number of sculpin with unequal numbers of trout and sculpin; addition of trout to a constant number of sculpin with equal numbers of trout and sculpin; and replacement of half the sculpin with an equal number of trout) to investigate the relative strength of interspecific versus intraspecific interactions. 3. Presence of fish (all three species, alone or in combined-species treatments) was not associated with changes in total dry mass of benthic invertebrates or shifts in relative abundance of benthic invertebrate taxa, regardless of fish density design. 4. Brook trout and sculpin diets did not change when each species was alone compared with treatments of both species together. Likewise, we did not find evidence for shifts in brown trout or sculpin diets when each species was alone or together. 5. We suggest that native brook trout and non-native brown trout fill similar niches in Valley Creek. We did not find evidence that either species had an effect on stream communities, potentially due to high invertebrate productivity in Valley Creek. ?? 2007 Blackwell Publishing Ltd.

  6. Hydrogen ion input to the Hubbard Brook Experimental Forest, New Hampshire, during the last decade

    Science.gov (United States)

    Gene E. Likens; F. Herbert Bormann; John S. Eaton; Robert S. Pierce; Noye M. Johnson

    1976-01-01

    Being downwind of eastern and midwestern industrial centers, the Hubbard Brook Experimental Forest offers a prime location to monitor long-term trends in atmospheric chemistry. Continuous measurements of precipitation chemistry during the last 10 years provide a measure of recent changes in precipitation inputs of hydrogen ion. The weighted average pH of precipitation...

  7. Impaired swimming performance of acid-exposed Arctic charr, Salvelinus alpinus L

    Energy Technology Data Exchange (ETDEWEB)

    Hunter, L.A. (North/South Consultants Inc., Winnipeg, MB (Canada)); Scherer, E. (Dept. of Fisheries and Oceans, Freshwater Inst. Science Lab., Winnipeg, MB (Canada))

    1988-01-01

    Effects of increased ambient acidity are of particular interest, as the formation of metabolic and respiratory acids and acceleration of branchial ion loss during vigorous swimming duplicates or compounds effects of exposure to environmental acidity. Three year old Arctic charr (Salvelinus alpinus L.) were exposed to five levels of acidity between pH 6 and pH 3.8. Swimming performance as determined by critical swimming speeds was 67.5 cm {center dot} s{sup -1} or 4.4 body lengths per second for untreated fish (pH 7.8). Performance declined sharply below pH 4.5; at pH 3.8 it was reduced by 35% after 7 days of exposure. Tailbeat frequencies and ventilation rates showed no dose-response effects. This would support the assumption that afferent and efferent neuromuscular functions may have remained unimpaired under increased ambient acidity so that the stimulus of directed water current continued to elicit forced swimming, causing (forcing) the fish to use the entire scope for activity available at the various pH levels. At swimming speeds between 20 and 50 cm {center dot} s{sup -1}, ventilation rates at all levels of acidity were higher than at the control level. Based on this, spontaneous, i.e., non-forced swimming activity may show a lower response threshold. 19 refs., 3 figs., 1 tab.

  8. Climate change and vulnerability of bull trout (Salvelinus confluentus) in a fire-prone landscape.

    Science.gov (United States)

    Falke, Jeffrey A.; Flitcroft, Rebecca L; Dunham, Jason B.; McNyset, Kristina M.; Hessburg, Paul F.; Reeves, Gordon H.

    2015-01-01

    Linked atmospheric and wildfire changes will complicate future management of native coldwater fishes in fire-prone landscapes, and new approaches to management that incorporate uncertainty are needed to address this challenge. We used a Bayesian network (BN) approach to evaluate population vulnerability of bull trout (Salvelinus confluentus) in the Wenatchee River basin, Washington, USA, under current and future climate and fire scenarios. The BN was based on modeled estimates of wildfire, water temperature, and physical habitat prior to, and following, simulated fires throughout the basin. We found that bull trout population vulnerability depended on the extent to which climate effects can be at least partially offset by managing factors such as habitat connectivity and fire size. Moreover, our analysis showed that local management can significantly reduce the vulnerability of bull trout to climate change given appropriate management actions. Tools such as our BN that explicitly integrate the linked nature of climate and wildfire, and incorporate uncertainty in both input data and vulnerability estimates, will be vital in effective future management to conserve native coldwater fishes.

  9. Lake trout otolith chronologies as multidecadal indicators of high-latitude freshwater ecosystems

    Science.gov (United States)

    Black, B.A.; Von Biela, V.R.; Zimmerman, C.E.; Brown, Randy J.

    2013-01-01

    High-latitude ecosystems are among the most vulnerable to long-term climate change, yet continuous, multidecadal indicators by which to gauge effects on biology are scarce, especially in freshwater environments. To address this issue, dendrochronology (tree-ring analysis) techniques were applied to growth-increment widths in otoliths from lake trout (Salvelinus namaycush) from the Chandler Lake system, Alaska (68.23°N, 152.70°W). All otoliths were collected in 1987 and exhibited highly synchronous patterns in growth-increment width. Increments were dated, the widths were measured, and age-related growth declines were removed using standard dendrochronology techniques. The detrended time series were averaged to generate an annually resolved chronology, which continuously spanned 1964–1984. The chronology positively and linearly correlated with August air temperature over the 22-year interval (p fish metabolic rate or lake productivity. Given the broad distribution of lake trout within North America, this study suggests that otolith chronologies could be used to examine responses between freshwater ecosystems and environmental variability across a range of temporal and spatial scales.

  10. Xenobiotic-induced apoptosis: significance and potential application as a general biomarker of response

    Science.gov (United States)

    Sweet, Leonard I.; Passino-Reader, Dora R.; Meier, Peter G.; Omann, Geneva M.

    1999-01-01

    The process of apoptosis, often coined programmed cell death, involves cell injury induced by a variety of stimuli including xenobiotics and is morphologically, biochemically, and physiologically distinct from necrosis. Apoptotic death is characterized by cellular changes such as cytoplasm shrinkage, chromatin condensation, and plasma membrane asymmetry. This form of cell suicide is appealing as a general biomarker of response in that it is expressed in multiple cell systems (e.g. immune, neuronal, hepatal, intestinal, dermal, reproductive), is conserved phylogenetically (e.g. fish, rodents, birds, sheep, amphibians, roundworms, plants, humans), is modulated by environmentally relevant levels of chemical contaminants, and indicates a state of stress of the organism. Further, apoptosis is useful as a biomarker as it serves as a molecular control point and hence may provide mechanistic information on xenobiotic stress. Studies reviewed here suggest that apoptosis is a sensitive and early indicator of acute and chronic chemical stress, loss of cellular function and structure, and organismal health. Examples are provided of the application of this methodology in studies of health of lake trout (Salvelinus namaycush) in the Laurentian Great Lakes.

  11. Comparison of genetic and visual identification of cisco and lake whitefish larvae from Chaumont Bay, Lake Ontario

    Science.gov (United States)

    George, Ellen M.; Hare, Matthew P.; Crabtree, Darran L.; Lantry, Brian F.; Rudstam, Lars G.

    2017-01-01

    Cisco Coregonus artedi are an important component of native food webs in the Great Lakes, and their restoration is instrumental to the recovery of lake trout Salvelinus namaycush and Atlantic salmon Salmo salar. Difficulties with visual identification of larvae can confound early life history surveys, as cisco are often difficult to distinguish from lake whitefish C. clupeaformis. We compared traditional visual species identification methods to genetic identifications based on barcoding of the mitochondrial cytochrome C oxidase I gene for 726 coregonine larvae caught in Chaumont Bay, Lake Ontario. We found little agreement between the visual characteristics of cisco identified by genetic barcoding and the most widely used dichotomous key, and the considerable overlap in ranges of traditionally utilized metrics suggest that visual identification of coregonine larvae from Chaumont Bay is impractical. Coregonines are highly variable and plastic species, and often display wide variations in morphometric characteristics across their broad range. This study highlights the importance of developing accurate, geographically appropriate larval identification methods in order to best inform cisco restoration and management efforts.

  12. Congener Patterns of Persistent Organic Pollutants Establish the Extent of Contaminant Biotransport by Pacific Salmon in the Great Lakes.

    Science.gov (United States)

    Gerig, Brandon S; Chaloner, Dominic T; Janetski, David J; Rediske, Richard R; O'Keefe, James P; Moerke, Ashley H; Lamberti, Gary A

    2016-01-19

    In the Great Lakes, introduced Pacific salmon (Oncorhynchus spp.) can transport persistent organic pollutants (POPs), such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), to new environments during their spawning migrations. To explore the nature and extent of POP biotransport by salmon, we compared 58 PCB and 6 PBDE congeners found in spawning salmon directly to those in resident stream fish. We hypothesized that stream fish exposed to salmon spawners would have congener patterns similar to those of salmon, the presumed contaminant source. Using permutational multivariate analysis of variance (PERMANOVA) and nonmetric multidimensional scaling (NMDS), we found that POP congener patterns of Pacific salmon varied among regions in the Great Lakes basin (i.e., Lake Huron, Lake Michigan, or Lake Superior), tissue type (whole fish or eggs), and contaminant type (PCB or PBDE). For stream-resident fish, POP congener pattern was influenced by the presence of salmon, location (i.e., Great Lakes Basin), and species identity (i.e., brook trout [Salvelinus fontinalis] or mottled sculpin [Cottus bairdii]). Similarity in congener patterns indicated that salmon are a source of POPs to brook trout in stream reaches receiving salmon spawners from Lake Michigan and Lake Huron but not from Lake Superior. Congener patterns of mottled sculpin differed from those of brook trout and salmon, suggesting that brook trout and mottled sculpin either use salmon tissue to differing degrees, acquire POPs from different dietary sources, or bioaccumulate or metabolize POPs differently. Overall, our analyses identified the important role of salmon in contaminant biotransport but also demonstrated that the extent of salmon-mediated POP transfer and uptake in Great Lakes tributaries is location- and species-specific.

  13. Invasion by nonnative brook trout in Panther Creek, Idaho: Roles of habitat quality, connectivity, and biotic resistance

    Science.gov (United States)

    Joseph R. Benjamin

    2006-01-01

    Theoretical models suggest the invasion of nonnative freshwater species is facilitated through the interaction of three factors: biotic resistance, habitat quality, and connectivity. We measured variables that represented each component to determine which were associated with small (150 mm) brook trout occurrence in Panther Creek, a tributary...

  14. Comparison of organotin accumulation on the white-spotted charr Salvelinus leucomaenis between sea-run and freshwater-resident types

    Science.gov (United States)

    Ohji, Madoka; Harino, Hiroya; Arai, Takaomi

    2011-01-01

    To examine the accumulation pattern of organotin compounds (OTs) in relation to the migration of diadromous fish, tributyltin (TBT) and triphenyltin (TPT) compounds and their derivatives were determined in the muscle tissue of both sea-run (anadromous) and freshwater-resident (nonanadromous) types of the white-spotted charr Salvelinus leucomaenis. There were generally no significant correlations between the TBT and TPT accumulation and various biological characteristics such as the total length (TL), body weight (BW), age and sex in S. leucomaenis. It is noteworthy that the TBT and TPT concentrations in sea-run white-spotted charr were significantly higher than in freshwater-resident individuals, although they are intraspecies. These results suggest that the sea-run S. leucomaenis has a higher ecological risk of TBT and TPT exposure than the freshwater-residents during their life history.

  15. Organic Compounds in Running Gutter Brook Water Used for Public Supply near Hatfield, Massachusetts, 2003-05

    Science.gov (United States)

    Brown, Craig J.; Trombley, Thomas J.

    2009-01-01

    The 258 organic compounds studied in this U.S. Geological Survey (USGS) assessment generally are man-made, including pesticides, solvents, gasoline hydrocarbons, personal-care and domestic-use products, and pavement and combustion-derived compounds. Of these 258 compounds, 26 (about 10 percent) were detected at least once among the 31 samples collected approximately monthly during 2003-05 at the intake of a flowthrough reservoir on Running Gutter Brook in Massachusetts, one of several community water systems on tributaries of the Connecticut River. About 81 percent of the watershed is forested, 14 percent is agricultural land, and 5 percent is urban land. In most source-water samples collected at Running Gutter Brook, fewer compounds were detected and their concentrations were low (less than 0.1 micrograms per liter) when compared with compounds detected at other stream sites across the country that drain watersheds that have a larger percentage of agricultural and urban areas. The relatively few compounds detected at low concentrations reflect the largely undeveloped land use at Running Gutter Brook. Despite the absence of wastewater discharge points on the stream, however, the compounds that were detected could indicate different sources and uses (point sources, precipitation, domestic, and agricultural) and different pathways to drinking-water supplies (overland runoff, groundwater discharge, leaking of treated water from distribution lines, and formation during treatment). Six of the 10 compounds detected most commonly (in at least 20 percent of the samples) in source water also were detected commonly in finished water (after treatment but prior to distribution). Concentrations in source and finished water generally were below 0.1 micrograms per liter and always less than humanhealth benchmarks, which are available for about one-half of the compounds detected. On the basis of this screening-level assessment, adverse effects to human health are expected to be

  16. Levels-of-growing-stock cooperative study in Douglas-fir: report no. 18--Rocky Brook, 1963-2006.

    Science.gov (United States)

    Robert O. Curtis; David D. Marshall

    2009-01-01

    This report documents the history and results of the Rocky Brook installation of the cooperative levels-of-growing-stock (LOGS) study in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), over the 41-year period 1965 to 2006. This 1938 plantation is one of the two site-IV installations among the nine installations in the study. Much public...

  17. Elevation dependent sensitivity of northern hardwoods to Ca addition at Hubbard Brook Experimental Forest, NH USA

    Science.gov (United States)

    Rakesh Minocha; Stephanie Long; Palaniswamy Thangavel; Subhash C. Minocha; Christopher Eagar; Charles T. Driscoll

    2010-01-01

    Acidic deposition has caused a depletion of calcium (Ca) in the northeastern forest soils. Wollastonite (Ca silicate) was added to watershed 1 (WS1) at the Hubbard Brook Experimental Forest (HBEF) in 1999 to evaluate its effects on various functions of the HBEF ecosystem. The effects of Ca addition on foliar soluble (extractable in 5% HClO4) ions...

  18. What Do Facts Have to Do with It? Exploring Instructional Emphasis in Stony Brook News Literacy Curriculum

    Science.gov (United States)

    Fleming, Jennifer

    2015-01-01

    An analytic matrix comprised of multiple media literacy teaching and learning principles is conceptualized to examine a model of news literacy developed by journalism educators at Stony Brook University. The multidimensional analysis indicates that news literacy instructors focus on teaching students how to question and assess the veracity of news…

  19. Fish assemblage structure and relations with environmental conditions in a Rocky Mountain watershed

    Science.gov (United States)

    Quist, M.C.; Hubert, W.A.; Isaak, D.J.

    2004-01-01

    Fish and habitat were sampled from 110 reaches in the Salt River basin (Idaho and Wyoming) during 1996 and 1997 to assess patterns in fish assemblage structure across a Rocky Mountain watershed. We identified four distinct fish assemblages using cluster analysis: (1) allopatric cutthroat trout (Oncorhynchus clarki (Richardson, 1836)); (2) cutthroat trout - brook trout (Salvelinus fontinalis (Mitchell, 1814)) - Paiute sculpin (Cottus beldingi Eigenmann and Eigenmann, 1891); (3) cutthroat trout - brown trout (Salmo trutta L., 1758) - mottled sculpin (Cottus bairdi Girard, 1850); and (4) Cyprinidae-Catostomidae. The distribution of fish assemblages was explained by thermal characteristics, stream geomorphology, and local habitat features. Reaches with allopatric cutthroat trout and the cutthroat trout - brook trout - Paiute sculpin assemblage were located in high-elevation, high-gradient streams. The other two fish assemblages were generally located in low-elevation streams. Associations between habitat gradients, locations of reaches in the watershed, and occurrence of species were further examined using canonical correspondence analysis. The results suggest that stream geomorphology, thermal conditions, and local habitat characteristics influence fish assemblage structure across a Rocky Mountain watershed, and they provide information on the ecology of individual species that can guide conservation activities. ?? 2004 NRC Canada.

  20. Behind the impact of introduced trout in high altitude lakes: adult, not juvenile fish are responsible of the selective predation on crustacean zooplankton

    Directory of Open Access Journals (Sweden)

    Rocco Tiberti

    2014-05-01

    Full Text Available Introduced fish seriously affect zooplankton communities in mountain lakes, often leading to the loss of large species. Selective predation is recognized to be the ultimate cause of such a strong impact. Here we describe the selection of zooplankton prey by analyzing the stomach contents of more than 300 brook trout (Salvelinus fontinalis inhabiting seven alpine lakes in the Gran Paradiso National Park (western Italian Alps. Our results show that planktivory is much more common in young fish, which feed on a larger number of taxa, but also adult fish maintain the ability to feed on zooplankton. There is a direct dependence between the length of zooplankton prey and the length of their fish predators, and adult, not juvenile fish are responsible of the selective predation on large crustacean zooplankton, which drive the impact of introduced fish throughout the entire zooplankton community. In some rare cases, large zooplankton populations develop in the presence of brook trout, and planktivory can become an important temporary resource for adult fish during the ice-free season. Thus, in the early stages of the establishment of non-native trout in alpine lakes, large-bodied zooplankton may represent an important food resource.

  1. HPLC Quantification of astaxanthin and canthaxanthin in Salmonidae eggs.

    Science.gov (United States)

    Tzanova, Milena; Argirova, Mariana; Atanasov, Vasil

    2017-04-01

    Astaxanthin and canthaxanthin are naturally occurring antioxidants referred to as xanthophylls. They are used as food additives in fish farms to improve the organoleptic qualities of salmonid products and to prevent reproductive diseases. This study reports the development and single-laboratory validation of a rapid method for quantification of astaxanthin and canthaxanthin in eggs of rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis М.). An advantage of the proposed method is the perfect combination of selective extraction of the xanthophylls and analysis of the extract by high-performance liquid chromatography and photodiode array detection. The method validation was carried out in terms of linearity, accuracy, precision, recovery and limits of detection and quantification. The method was applied for simultaneous quantification of the two xanthophylls in eggs of rainbow trout and brook trout after their selective extraction. The results show that astaxanthin accumulations in salmonid fish eggs are larger than those of canthaxanthin. As the levels of these two xanthophylls affect fish fertility, this method can be used to improve the nutritional quality and to minimize the occurrence of the M74 syndrome in fish populations. Copyright © 2016 John Wiley & Sons, Ltd.

  2. Evaluation of erythrocyte delta-amino levulinic acid dehydratase activity as a short-term indicator in fish of a harmful exposure to lead

    Energy Technology Data Exchange (ETDEWEB)

    Hodson, P.V.; Blunt, B.R.; Spry, D.J.; Austen, K.

    1977-04-01

    The activity of erythrocyte delta-amino levulinic acid dehydratase (ALA-D) of fish is easily measured under a variety of experimental conditions. Exposure of rainbow trout (Salmo gairdneri), brook trout (Salvelinus fontinalis), goldfish (Carassius auratus), and pumpkinseeds (Lepomis gibbosus) to lead consistently inhibited ALA-D within 2 wks at concentrations as low as 10, 90, 470, and 90 ..mu..g/l, respectively. In rainbow and brook trout these concentrations were closely related to the published minimum effective concentrations causing sublethal harm. There was a significant linear relationship between ALA-D activity and log of blood lead concentration, between ALA-D activity and log of lead in water, and between blood lead and lead in water. Near lethal exposures to cadmium, copper, zinc, and mercury did not significantly inhibit ALA-D activity. Recovery of ALA-D activity of rainbow trout after transfer from 120 ..mu..g/l lead to clean water occurred in 8 wk. This enzyme provides fast, consistent, specific, and sensitive estimates of lead concentrations causing sublethal harm to fish and may help to relate sources of lead to degree of exposure of fish populations in the field.

  3. Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus.

    Directory of Open Access Journals (Sweden)

    Alicia A Cassidy

    Full Text Available Protein metabolism, including the interrelated processes of synthesis and degradation, mediates the growth of an animal. In ectothermic animals, protein metabolism is responsive to changes in both biotic and abiotic conditions. This study aimed to characterise responses of protein metabolism to food deprivation that occur in the coldwater salmonid, Arctic charr, Salvelinus alpinus. We compared two groups of Arctic charr: one fed continuously and the other deprived of food for 36 days. We measured the fractional rate of protein synthesis (KS in individuals from the fed and fasted groups using a flooding dose technique modified for the use of deuterium-labelled phenylalanine. The enzyme activities of the three major protein degradation pathways (ubiquitin proteasome, lysosomal cathepsins and the calpain systems were measured in the same fish. This study is the first to measure both KS and the enzymatic activity of protein degradation in the same fish, allowing us to examine the apparent contribution of different protein degradation pathways to protein turnover in various tissues (red and white muscle, liver, heart and gills. KS was lower in the white muscle and in liver of the fasted fish compared to the fed fish. There were no observable effects of food deprivation on the protease activities in any of the tissues with the exception of liver, where the ubiquitin proteasome pathway seemed to be activated during fasting conditions. Lysosomal proteolysis appears to be the primary degradation pathway for muscle protein, while the ubiquitin proteasome pathway seems to predominate in the liver. We speculate that Arctic charr regulate protein metabolism during food deprivation to conserve proteins.

  4. Multiscale hydrogeomorphic influences on bull trout (Salvelinus confluentus) spawning habitat

    Science.gov (United States)

    Bean, Jared R; Wilcox, Andrew C.; Woessner, William W.; Muhlfeld, Clint C.

    2015-01-01

    We investigated multiscale hydrogeomorphic influences on the distribution and abundance of bull trout (Salvelinus confluentus) spawning in snowmelt-dominated streams of the upper Flathead River basin, northwestern Montana. Within our study reaches, bull trout tended to spawn in the finest available gravel substrates. Analysis of the mobility of these substrates, based on one-dimensional hydraulic modeling and calculation of dimensionless shear stresses, indicated that bed materials in spawning reaches would be mobilized at moderate (i.e., 2-year recurrence interval) high-flow conditions, although the asynchronous timing of the fall–winter egg incubation period and typical late spring – early summer snowmelt high flows in our study area may limit susceptibility to redd scour under current hydrologic regimes. Redd occurrence also tended to be associated with concave-up bedforms (pool tailouts) with downwelling intragravel flows. Streambed temperatures tracked stream water diurnal temperature cycles to a depth of at least 25 cm, averaging 6.1–8.1 °C in different study reaches during the spawning period. Ground water provided thermal moderation of stream water for several high-density spawning reaches. Bull trout redds were more frequent in unconfined alluvial valley reaches (8.5 versus 5.0 redds·km−1 in confined valley reaches), which were strongly influenced by hyporheic and groundwater – stream water exchange. A considerable proportion of redds were patchily distributed in confined valley reaches, however, emphasizing the influence of local physical conditions in supporting bull trout spawning habitat. Moreover, narrowing or “bounding” of these alluvial valley segments did not appear to be important. Our results suggest that geomorphic, thermal, and hydrological factors influence bull trout spawning occurrence at multiple spatial scales.

  5. 77 FR 37707 - Draft Environmental Impact Statement on Brooks River Visitor Access for Katmai National Park and...

    Science.gov (United States)

    2012-06-22

    ... four action alternatives that include bridge and boardwalk systems to replace the existing Brooks River floating bridge and sites to relocate the existing Naknek Lake barge landing area at the mouth of the... maintain seasonal use of the floating bridge, which is 8 feet wide and about 320 feet long. The bridge...

  6. Final-Independent Confirmatory Survey Report For The Reactor Building, Hot Laboratory, Primary Pump House, And Land Areas At The Plum Brook Reactor Facility, Sandusky, Ohio DCN:2036-SR-01-10

    International Nuclear Information System (INIS)

    Bailey, Erika N.

    2011-01-01

    In 1941, the War Department acquired approximately 9,000 acres of land near Sandusky, Ohio and constructed a munitions plant. The Plum Brook Ordnance Works Plant produced munitions, such as TNT, until the end of World War II. Following the war, the land remained idle until the National Advisory Committee for Aeronautics later called the National Aeronautics and Space Administration (NASA) obtained 500 acres to construct a nuclear research reactor designed to study the effects of radiation on materials used in space flight. The research reactor was put into operation in 1961 and was the first of fifteen test facilities eventually built by NASA at the Plum Brook Station. By 1963, NASA had acquired the remaining land at Plum Brook for these additional test facilities

  7. Exposure-related effects of Pseudomonas fluorescens, strain CL145A, on coldwater, coolwater, and warmwater fish

    Science.gov (United States)

    Luoma, James A.; Weber, Kerry L.; Denise A. Mayer,

    2015-01-01

    The exposure-related effects of a commercially prepared spray-dried powder (SDP) formulation of Pseudomonas fluorescens, strain CL145A, were evaluated on coldwater, coolwater, and warmwater fish endemic to the Great Lakes and Upper Mississippi River Basins. Nine species of young-of-the-year fish were exposed to SDP for 24 hours by using continuous-flow, serial-dilution exposure systems at temperatures of 12 degrees Celsius (°C; 2 species; Oncorhynchus mykiss [rainbow trout] and Salvelinus fontinalis [brook trout]), 17 °C (3 species; Perca flavescens [yellow perch], Sander vitreus [walleye], and Acipenser fulvescens [lake sturgeon]), or 22 °C (4 species; Micropterus salmoides [largemouth bass], Micropterus dolomieu [smallmouth bass], Lepomis macrochirus [bluegill sunfish], and Ictalurus punctatus [channel catfish]).

  8. Biomagnification of mercury through lake trout (Salvelinus namaycush) food webs of lakes with different physical, chemical and biological characteristics

    Energy Technology Data Exchange (ETDEWEB)

    Kidd, Karen A., E-mail: kiddk@unbsj.ca [Canadian Rivers Institute and Biology Department, University of New Brunswick, Saint John, NB, Canada E2L 4L5 (Canada); Muir, Derek C.G., E-mail: derek.muir@ec.gc.ca [Aquatic Contaminants Research Division, Environment Canada, Burlington, ON, Canada L7R 4A6 (Canada); Evans, Marlene S., E-mail: marlene.evans@ec.gc.ca [Aquatic Contaminants Research Division, Environment Canada, Saskatoon, SK, Canada S7N 3H5 (Canada); Wang, Xioawa, E-mail: xiaowa.wang@ec.gc.ca [Aquatic Contaminants Research Division, Environment Canada, Burlington, ON, Canada L7R 4A6 (Canada); Whittle, Mike [Aquatic Contaminants Research Division, Environment Canada, Burlington, ON, Canada L7R 4A6 (Canada); Swanson, Heidi K., E-mail: heidikswanson@yahoo.ca [Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9 (Canada); Johnston, Tom, E-mail: tjohnston@laurentian.ca [Cooperative Freshwater Ecology Unit, Ontario Ministry of Natural Resources, and Biology Department, Laurentian University, Sudbury, ON, Canada P3E 2C6 (Canada); Guildford, Stephanie, E-mail: sguildfo@d.umn.edu [Department of Biology and Large Lakes Observatory, University of Minnesota Duluth, 2205 5th St., Duluth, MN, 55812 (United States)

    2012-11-01

    Mercury (Hg) biomagnification in aquatic ecosystems remains a concern because this pollutant is known to affect the health of fish-eating wildlife and humans, and the fish themselves. The 'rate' of mercury biomagnification is being assessed more frequently using stable nitrogen isotope ratios ({delta}{sup 15}N), a measure of relative trophic position of biota within a food web. Within food webs and across diverse systems, log-transformed Hg concentrations are significantly and positively related to {delta}{sup 15}N and the slopes of these models vary from one study to another for reasons that are not yet understood. Here we compared the rates of Hg biomagnification in 14 lake trout lakes from three provinces in Canada to understand whether any characteristics of the ecosystems explained this among-system variability. Several fish species, zooplankton and benthic invertebrates were collected from these lakes and analyzed for total Hg (fish only), methyl Hg (invertebrates) and stable isotopes ({delta}{sup 15}N; {delta}{sup 13}C to assess energy sources). Mercury biomagnification rates varied significantly across systems and were higher for food webs of larger (surface area), higher nutrient lakes. However, the slopes were not predictive of among-lake differences in Hg in the lake trout. Results indicate that among-system differences in the rates of Hg biomagnification seen in the literature may be due, in part, to differences in ecosystem characteristics although the mechanisms for this variability are not yet understood. -- Highlights: Black-Right-Pointing-Pointer Mercury biomagnifies through aquatic food webs to toxic levels in top predator fishes. Black-Right-Pointing-Pointer Among-system differences in mercury transfer through food webs occur but have not been explained. Black-Right-Pointing-Pointer Diverse lakes supporting lake trout were compared to understand the ecosystem processes that affect mercury biomagnification. Black-Right-Pointing-Pointer Higher biomagnification of mercury was found in larger, higher nutrient lakes. Black-Right-Pointing-Pointer Results show that the food web processing of mercury is related to ecosystem properties.

  9. A Quantitative Polymerase Chain Reaction Assay for the Detection and Quantification of Epizootic Epitheliotropic Disease Virus (EEDV; Salmonid Herpesvirus 3).

    Science.gov (United States)

    Glenney, Gavin W; Barbash, Patricia A; Coll, John A

    2016-03-01

    Epizootic epitheliotropic disease virus (EEDV; salmonid herpesvirus [SalHV3]; family Alloherpesviridae) causes a systemic disease of juvenile and yearling Lake Trout Salvelinus namaycush. No cell lines are currently available for the culture and propagation of EEDV, so primary diagnosis is limited to PCR and electron microscopy. To better understand the pervasiveness of EEDV (carrier or latent state of infection) in domesticated and wild Lake Trout populations, we developed a sensitive TaqMan quantitative PCR (qPCR) assay to detect the presence of the EEDV terminase gene in Lake Trout tissues. This assay was able to detect a linear standard curve over nine logs of plasmid dilution and was sensitive enough to detect single-digit copies of EEDV. The efficiency of the PCR assay was 99.4 ± 0.06% (mean ± SD), with a 95% confidence limit of 0.0296 (R(2) = 0.994). Methods were successfully applied to collect preliminary data from a number of species and water bodies in the states of Pennsylvania, New York, and Vermont, indicating that EEDV is more common in wild fish than previously known. In addition, through the development of this qPCR assay, we detected EEDV in a new salmonid species, the Cisco Coregonus artedi. The qPCR assay was unexpectedly able to detect two additional herpesviruses, the Atlantic Salmon papillomatosis virus (ASPV; SalHV4) and the Namaycush herpesvirus (NamHV; SalHV5), which both share high sequence identity with the EEDV terminase gene. With these unexpected findings, we subsequently designed three primer sets to confirm initial TaqMan qPCR assay positives and to differentiate among EEDV, ASPV, and NamHV by detecting the glycoprotein genes via SYBR Green qPCR. Received April 20, 2015; accepted November 10, 2015.

  10. Technical Note: Reliability of Suchey-Brooks and Buckberry-Chamberlain methods on 3D visualizations from CT and laser scans

    DEFF Research Database (Denmark)

    Villa, Chiara; Buckberry, Jo; Cattaneo, Cristina

    2013-01-01

    Previous studies have reported that the ageing method of Suchey-Brooks (pubic bone) and some of the features applied by Lovejoy et al. and Buckberry-Chamberlain (auricular surface) can be confidently performed on 3D visualizations from CT-scans. In this study, seven observers applied the Suchey......-Brooks and the Buckberry-Chamberlain methods on 3D visualizations based on CT-scans and, for the first time, on 3D visualizations from laser scans. We examined how the bone features can be evaluated on 3D visualizations and whether the different modalities (direct observations of bones, 3D visualization from CT......-observer agreement was obtained in the evaluation of the pubic bone in all modalities. In 3D visualizations of the auricular surfaces, transverse organization and apical changes could be evaluated, although with high inter-observer variability; micro-, macroporosity and surface texture were very difficult to score...

  11. Flood inundation maps and water-surface profiles for tropical storm Irene and selected annual exceedance probability floods for Flint Brook and the Third Branch White River in Roxbury, Vermont

    Science.gov (United States)

    Ahearn, Elizabeth A.; Lombard, Pamela J.

    2014-01-01

    Flint Brook, a tributary to the Third Branch White River in Roxbury, Vermont, has a history of flooding the Vermont Fish and Wildlife Department’s Roxbury Fish Culture Station (the hatchery) and surrounding infrastructure. Flooding resulting from tropical storm Irene on August 28–29, 2011, caused widespread destruction in the region, including extensive and costly damages to the State-owned hatchery and the transportation infrastructure in the Town of Roxbury, Vermont. Sections of State Route 12A were washed out, and several bridges and culverts on Oxbow Road, Thurston Hill Road, and the New England Central Railroad in Roxbury were heavily damaged. Record high peak-discharge estimates of 2,140 cubic feet per second (ft3/s) and 4,320 ft3/s were calculated for Flint Brook at its confluence with the Third Branch White River and for the Third Branch White River at about 350 feet (ft) downstream from the hatchery, respectively. The annual exceedance probabilities (AEPs) of the peak discharges for Flint Brook and the Third Branch White River were less than 0.2 percent (less than a one in 500 chance of occurring in a given year). Hydrologic and hydraulic analyses of Flint Brook and the Third Branch White River were done to investigate flooding at the hatchery in Roxbury and support efforts by the Federal Emergency Management Agency to assist State and local mitigation and reconstruction efforts. During the August 2011 flood, the majority of flow from Flint Brook (97 percent or 2,070 ft3/s) diverged from its primary watercourse due to a retaining wall failure immediately upstream of Oxbow Road and inundated the hatchery. Although a minor amount of flow from the Third Branch White River could have overtopped State Route 12A and spilled into the hatchery, the Third Branch White River did not cause flood damages or exacerbate flooding at the hatchery during the August 2011 flood. The Third Branch White River which flows adjacent to the hatchery does not flood the hatchery

  12. A Statistical Analysis of the Relationship of Distance and Mode of Transportation on Length of Stay at Brooke Army Medical Center

    National Research Council Canada - National Science Library

    Hagen, John

    1997-01-01

    .... Transportation Command's (TRANSCOM) aeromedical evacuation system, to determine their influence on length of hospital stay at Brooke Army Medical Center in FY 1996 in order to better understand the irnpact these patients have on utilization management...

  13. Swimming behaviour and ascent paths of brook trout in a corrugated culvert

    Science.gov (United States)

    Goerig, Elsa; Bergeron, Normand E.; Castro-Santos, Theodore R.

    2017-01-01

    Culverts may restrict fish movements under some hydraulic conditions such as shallow flow depths or high velocities. Although swimming capacity imposes limits to passage performance, behaviour also plays an important role in the ability of fish to overcome velocity barriers. Corrugated metal culverts are characterized by unsteady flow and existence of low‐velocity zones, which can improve passage success. Here, we describe swimming behaviour and ascent paths of 148 wild brook trout in a 1.5‐m section of a corrugated metal culvert located in Raquette Stream, Québec, Canada. Five passage trials were conducted in mid‐August, corresponding to specific mean cross‐sectional flow velocities ranging from 0.30 to 0.63 m/s. Fish were individually introduced to the culvert and their movements recorded with a camera located above the water. Lateral and longitudinal positions were recorded at a rate of 3 Hz in order to identify ascent paths. These positions were related to the distribution of flow depths and velocities in the culvert. Brook trout selected flow velocities from 0.2 to 0.5 m/s during their ascents, which corresponded to the available flow velocities in the culvert at the low‐flow conditions. This however resulted in the use of low‐velocity zones at higher flows, mainly located along the walls of the culvert. Some fish also used the corrugations for sheltering, although the behaviour was marginal and did not occur at the highest flow condition. This study improves knowledge on fish behaviour during culvert ascents, which is an important aspect for developing reliable and accurate estimates of fish passage ability.

  14. Quantitative evaluation of macrophage aggregates in brook trout Salvelinus fontinalis and rainbow trout Oncorhynchus mykiss

    Science.gov (United States)

    Macrophage aggregates (MAs) occur in various organs of fishes, especially the kidney, liver and spleen, and contain melanin, ceroid/lipofuscin and hemosiderin pigments. They have been used as indicators of a number of natural and anthropogenic stressors. Macrophage aggregates occ...

  15. Eleven-year response of foliar chemistry to chronic nitrogen and sulfur additions at the Bear Brook Watershed in Maine

    Science.gov (United States)

    Jose Alexander Elvir; Lindsey Rustad; G. Bruce Wiersma; Ivan Fernandez; Alan S. White; Gregory J. White

    2005-01-01

    The foliar chemistry of sugar maple (Acer saccharum Marsh.), American beech (Fagus grandifolia Ehrh.), and red spruce (Picea rubens Sarg.) was studied from 1993 to 2003 at the Bear Brook Watershed in Maine (BBWM). The BBWM is a paired-watershed forest ecosystem study, with one watershed treated bimonthly since...

  16. Two new species of Dendrobrachia Brook, 1889 (Cnidaria: Octocorallia: Dendrobrachiidae from the north-eastern Atlantic and western Mediterranean

    Directory of Open Access Journals (Sweden)

    Pablo J. López-González

    2010-08-01

    Full Text Available Examination of recent benthic material collected during several cruises in the Gulf of Cadiz (NE Atlantic and the Strait of Sicily (Mediterranean has allowed the taxonomic reassessment of some previously identified specimens belonging to the monogeneric gorgonian family Dendrobrachiidae Brook, 1889. Dendrobrachia fallax Brook, 1889 is the type species of the single genus in this family, and was originally described from Ascension Island (South Atlantic. Subsequently, other authors reported the presence of this species in Cape Verde Islands (north-eastern Atlantic and some Mediterranean localities (Alboran Sea and the Strait of Sicily. The study of the specimen from the Prince of Monaco collections in Cape Verde Islands, and recently collected material from the Gulf of Cadiz (north-eastern Atlantic and in the south of Malta (Mediterranean, materials previously considered as D. fallax, allow us to recognize two undescribed species in this genus. All previous records of D. fallax from the north-eastern Atlantic and Mediterranean should be considered incorrect. Opresko and Bayer (1991 added two additional species of Dendrobrachia. Two new species are described here and compared with their congeners.

  17. K-Ar geochronology of the Survey Pass, Ambler River and Eastern Baird Mountains quadrangles, southwestern Brooks Range, Alaska

    Science.gov (United States)

    Turner, Donald L.; Forbes, R.B.; Mayfield, C.F.

    1978-01-01

    We report 76 previously unpublished K-Ar mineral ages from 47 metamorphic and igneous rocks in the southwestern Brooks Range. The pattern of radiometric ages is complex, reflecting the complex geologic history of this area. Local and regional radiometric evidence suggests that the southern Brooks Range schist belt has, at least in part, undergone a late Precambrian metamorphism and that the parent sedimentary and igneous rocks for the metamorphic rocks dated as late Precambrian are at least this old (Precambrian Z). This schist terrane experienced a major thermal event in mid-Cretaceous time, causing widespread resetting of nearly all K-Ar mica ages. A series of apparent ages intermediate between late Precambrian and mid-Cretaceous are interpreted as indicating varying amounts of partial argon loss from older rocks during the Cretaceous event. The schist belt is characterized by dominant metasediments and subordinate metabasites and metafelsites. Blueschists occur within the schist belt from the Chandalar quadrangle westward to the Baird Mountains quadrangle, but geologic evidence does not support the existence of a fossil subduction zone.

  18. Snake River sockeye salmon habitat and limnological research: Annual report 1997

    International Nuclear Information System (INIS)

    Taki, D.; Lewis, B.; Griswold, B.

    1999-01-01

    Since the late 1980's, Snake River sockeye Oncorhynchus nerka adults have only returned to Redfish Lake, one of five lakes in the Sawtooth Basin which historically reared sockeye. 1997 project objectives included (1) characterization of the limnology of Sawtooth Valley lakes; (2) fertilization of Redfish, Pettit, and Alturas lakes; (3) O.nerka lake population surveys; (4) estimation of kokanee escapement and fry production in Alturas Lake Creek, Stanley Lake Creek, and Fishhook Creek; (5) reduce the number of spawning kokanee in Fishook Creek; (6) evaluate hatchery rainbow trout overwinter survival and potential competition and predation interactions with O.nerka in Pettit Lake; (7) assess predation from bull trout Salvelinus malma, brook trout S.fontinalis, and northern squawfish Ptychocheilus oregonsis on lentic O.nerka; (8) establish screw tap and weir sites to monitor smolt emigration

  19. Lake Ontario: Food web dynamics in a changing ecosystem (1970-2000)

    Science.gov (United States)

    Mills, E.L.; Casselman, J.M.; Dermott, R.; Fitzsimons, J.D.; Gal, G.; Holeck, K. T.; Hoyle, J.A.; Johannsson, O.E.; Lantry, B.F.; Makarewicz, J.C.; Millard, E.S.; Munawar, I.F.; Munawar, M.; O'Gorman, R.; Owens, R.W.; Rudstam, L. G.; Schaner, T.; Stewart, T.J.

    2003-01-01

    We examined stressors that have led to profound ecological changes in the Lake Ontario ecosystem and its fish community since 1970. The most notable changes have been reductions in phosphorus loading, invasion by Dreissena spp., fisheries management through stocking of exotic salmonids and control of sea lamprey (Petromyzon marinus), and fish harvest by anglers and double-crested cormorants (Phalacrocorax auritus). The response to these stressors has led to (i) declines in both algal photosynthesis and epilimnetic zooplankton production, (ii) decreases in alewife (Alosa pseudoharengus) abundance, (iii) declines in native Diporeia and lake whitefish (Coregonus clupeaformis), (iv) behavioral shifts in alewife spatial distribution benefitting native lake trout (Salvelinus namaycush), threespine stickleback (Gasterosteus aculeatus), and emerald shiner (Notropis atherinoides) populations, (v) dramatic increases in water clarity, (vi) predation impacts by cormorants on select fish species, and (vii) lake trout recruitment bottlenecks associated with alewife-induced thiamine deficiency. We expect stressor responses associated with anthropogenic forces like exotic species invasions and global climate warming to continue to impact the Lake Ontario ecosystem in the future and recommend continuous long-term ecological studies to enhance scientific understanding and management of this important resource.

  20. Geochemical pathways and biological uptake of radium in small Canadian Shield lakes

    International Nuclear Information System (INIS)

    Hesslein, R.H.; Slavicek, E.

    1984-01-01

    The sediment-water interactions and biological uptake of 226 Ra are described for four small Canadian Shield lakes at the Experimental Lakes Area, Kenora, Ontario. A single addition of 226 Ra was made to each lake between 1970 and 1976. Approximately 90 percent of the added 226 Ra initially sorbed to the sediments. Outflow from the lakes showed losses of only 5-11 percent 226 Ra per year. Models are proposed for adsorption and outflow of 226 Ra from lakes. Biological uptake and long-term 226 Ra concentrations were measured in three species of macrophytes, crayfish, and five species of fish. Bioaccumulation ranged from 1100 to 5000 in macrophytes, 705 in crayfish, from 30 to 80 in large trout (Salvelinus namaycush), white sucker (Catostomus commersoni), and lake whitefish (Coregonus clupeaformis), and from 230 to 1200 in fathead minnows (Pimephales promelas), pearl dace (Semotilus margarita), and northern redbelly dace (Chrosomus eos). The concept of Ra/Ca ratio in organisms versus water and food is used to explain the differences in bioaccumulation. 226 Ra is discriminated against versus calcium by fish but favoured by macrophytes and crayfish

  1. Ethyl-p-aminobenzoate (Benzocaine): efficacy as an anesthetic for five species of freshwater fish

    Science.gov (United States)

    Dawson, V.K.; Gilderhus, P.A.

    1979-01-01

    Ethyl-p-aminobenzoate (benzocaine) was tested for its efficacy as an anesthetic for rainbow trout (Salmo gairdnerii, brown trout (Salmo truttas, northern pike (Esox lucius). carp (Cyprinus carpio), and largemouth bass (Mieropterus salmoidesi. Since benzocaine is not water soluble, it was applied with acetone as a carrier. Concentrations of 100 to 200 mg!l were required for large adult northern pike, compared with 50 to 100 mg/l for small fish. Rates of sedation and recovery were slower in cold water than in warm water. Water hardness had little influence on the activity of benzocaine. Fish were anesthetized faster and recovered more slowly in acid than in alkaline water. Benzocaine produced deep anesthesia, but concentrations that rendered the fish handleable within 5 min were generally not safe for exposures longer than 15 min. Concentrations of benzocaine efficacious for fish were not acutely toxic to eggs of coho salmon (Oncorhynchus kisutch), chinook salmon (Oncorhynchus tshauiytschas, rainbow trout, brown trout, or lake trout (Salvelinus namaycush). Benzocaine is not registered for fishery use and is neither more effective nor safer than the registered anesthetic, tricaine methanesulfonate (MS-222l.

  2. Lake trout rehabilitation in Lake Erie: a case history

    Science.gov (United States)

    Cornelius, Floyd C.; Muth, Kenneth M.; Kenyon, Roger

    1995-01-01

    Native lake trout (Salvelinus namaycush) once thrived in the deep waters of eastern Lake Erie. The impact of nearly 70 years of unregulated exploitation and over 100 years of progressively severe cultural eutrophication resulted in the elimination of lake trout stocks by 1950. Early attempts to restore lake trout by stocking were unsuccessful in establishing a self-sustaining population. In the early 1980s, New York's Department of Environmental Conservation, Pennsylvania's Fish and Boat Commission, and the U.S. Fish and Wildlife Service entered into a cooperative program to rehabilitate lake trout in the eastern basin of Lake Erie. After 11 years of stocking selected strains of lake trout in U.S. waters, followed by effective sea lamprey control, lake trout appear to be successfully recolonizing their native habitat. Adult stocks have built up significantly and are expanding their range in the lake. Preliminary investigations suggest that lake trout reproductive habitat is still adequate for natural reproduction, but natural recruitment has not been documented. Future assessments will be directed toward evaluation of spawning success and tracking age-class cohorts as they move through the fishery.

  3. Can pelagic forage fish and spawning cisco (Coregonus artedi) biomass in the western arm of Lake Superior be assessed with a single summer survey?

    Science.gov (United States)

    Yule, D.L.; Stockwell, J.D.; Schreiner, D.R.; Evrard, L.M.; Balge, M.; Hrabik, T.R.

    2009-01-01

    Management efforts to rehabilitate lake trout Salvelinus namaycush in Lake Superior have been successful and the recent increase in their numbers has led to interest in measuring biomass of pelagic prey fish species important to these predators. Lake Superior cisco Coregonus artedi currently support roe fisheries and determining the sustainability of these fisheries is an important management issue. We conducted acoustic and midwater trawl surveys of the western arm of Lake Superior during three periods: summer (July-August), October, and November 2006 to determine if a single survey can be timed to estimate biomass of both prey fish and spawning cisco. We evaluated our methods by comparing observed trawl catches of small (cisco increased substantially in November, while small bloater Coregonus hoyi biomass was lower. We compared our summer 2006 estimates of total fish biomass to the results of a summer survey in 1997 and obtained similar results. We conclude that the temporal window for obtaining biomass estimates of pelagic prey species in the western arm of Lake Superior is wide (July through October), but estimating spawning cisco abundance is best done with a November survey.

  4. Mercury in freshwater fish of northeast North America--a geographic perspective based on fish tissue monitoring databases.

    Science.gov (United States)

    Kamman, Neil C; Burgess, Neil M; Driscoll, Charles T; Simonin, Howard A; Goodale, Wing; Linehan, Janice; Estabrook, Robert; Hutcheson, Michael; Major, Andrew; Scheuhammer, Anton M; Scruton, David A

    2005-03-01

    As part of an initiative to assemble and synthesize mercury (Hg) data from environmental matrices across northeastern North America, we analyzed a large dataset comprised of 15,305 records of fish tissue Hg data from 24 studies from New York State to Newfoundland. These data were summarized to provide mean Hg concentrations for 40 fish species and associated families. Detailed analyses were carried out using data for 13 species. Hg in fishes varied by geographic area, waterbody type, and waterbody. The four species with the highest mean Hg concentrations were muskellunge (Esox masquinongy), walleye (Sander vitreus), white perch (Morone americana), and northern pike (Esox luscius). Several species displayed elevated Hg concentrations in reservoirs, relative to lakes and rivers. Normalized deviations from mean tissue levels for yellow perch (Perca flavescens) and brook trout (Salvelinus fontinalis) were mapped, illustrating how Hg concentrations in these species varied across northeastern North America. Certain geographic regions showed generally below or above-average Hg concentrations in fish, while significant heterogeneity was evident across the landscape. The proportion of waterbodies exhibiting exceedances of USEPA's criterion for fish methylmercury ranged from 14% for standard-length brook trout fillets to 42% for standard-length yellow perch fillets. A preliminary correlation analysis showed that fish Hg concentrations were related to waterbody acidity and watershed size.

  5. ACIDIFICATION TRENDS AND THE EVOLUTION OF NEUTRALIZATION MECHANISMS THROUGH TIME AT THE BEAR BROOK WATERSHED IN MAINE (BBWM), U.S.A.

    Science.gov (United States)

    The paired catchment study at the forested Bear Brook Watershed in Maine (BBWM) U.S.A. documents interactions among short- to long-term processes of acidification. In 1987-1989, runoff from the two catchments was nearly identical in quality and quantity. Ammonium sulfate has been...

  6. On Conceptual Metaphor and the Flora and Fauna of Mind: Commentary on Brookes and Etkina; and Jeppsson, Haglund, and Amin

    Science.gov (United States)

    Sherin, Bruce

    2015-01-01

    In this commentary, the author presents his thoughts on two papers appearing in this special issue. The first, "The Importance of Language in Students' Reasoning about Heat in Thermodynamic Processes," by David T. Brookes and Eugenia Etkina (See: EJ1060728), and the second, "Varying Use of Conceptual Metaphors Across Levels of…

  7. Past and projected future changes in snowpack and soil frost at the Hubbard Brook Experimental Forest, New Hampshire, USA

    Science.gov (United States)

    John L. Campbell; Scott V. Ollinger; Gerald N. Flerchinger; Haley Wicklein; Katharine Hayhoe; Amey S. Bailey

    2010-01-01

    Long-term data from the Hubbard Brook Experimental Forest in New Hampshire show that air temperature has increased by about 1 °C over the last half century. The warmer climate has caused significant declines in snow depth, snow water equivalent and snow cover duration. Paradoxically, it has been suggested that warmer air temperatures may result in colder soils...

  8. Bioenergetic evaluation of diel vertical migration by bull trout (Salvelinus confluentus) in a thermally stratified reservoir

    Science.gov (United States)

    Eckmann, Madeleine; Dunham, Jason B.; Connor, Edward J.; Welch, Carmen A.

    2018-01-01

    Many species living in deeper lentic ecosystems exhibit daily movements that cycle through the water column, generally referred to as diel vertical migration (DVM). In this study, we applied bioenergetics modelling to evaluate growth as a hypothesis to explain DVM by bull trout (Salvelinus confluentus) in a thermally stratified reservoir (Ross Lake, WA, USA) during the peak of thermal stratification in July and August. Bioenergetics model parameters were derived from observed vertical distributions of temperature, prey and bull trout. Field sampling confirmed that bull trout prey almost exclusively on recently introduced redside shiner (Richardsonius balteatus). Model predictions revealed that deeper (>25 m) DVMs commonly exhibited by bull trout during peak thermal stratification cannot be explained by maximising growth. Survival, another common explanation for DVM, may have influenced bull trout depth use, but observations suggest there may be additional drivers of DVM. We propose these deeper summertime excursions may be partly explained by an alternative hypothesis: the importance of colder water for gametogenesis. In Ross Lake, reliance of bull trout on warm water prey (redside shiner) for consumption and growth poses a potential trade-off with the need for colder water for gametogenesis.

  9. Geohydrology of Brooks, Lowndes, and western Echols counties, Georgia

    Science.gov (United States)

    Krause, R.E.

    1979-01-01

    The principal artesian aquifer, a limestone of Eocene to Miocene age, is the main source of water supply for Brooks, Lowndes, and western Echols Counties in south Georgia. Pumpage of about 22 million gallons perday from this prolific aquifer has not posed any problems regarding declining water levels or depletion of the reservoir. However, water-quality problems do occur in the Valdosta area. Seepage-run measurements indicate that the Withlacoochee River north of Valdosta contributes an average of 112 cubic feet per second of water to caverns and sinkholes that recharge the aquifer. Wells near the recharge area withdraw relatively unfiltered water with iron concentration and color intensity exceeding standards for drinking water. South of Valdosta, water from the aquifer contains as much as 3.0 milligrams per liter of hydrogen sulfide, rendering the water unfit for drinking. Water high in sulfate concentration occurs below 550 feet in the lower part of the aquifer in Valdosta, and is assumed to be present at that depth throughout the study area. Generally, sufficient quantities of freshwater can be obtained without drilling to this depth.

  10. 75 FR 3217 - J&T Hydro Company; H. Dean Brooks and W. Bruce Cox; Notice of Application for Transfer of License...

    Science.gov (United States)

    2010-01-20

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 11392-009] J&T Hydro Company; H. Dean Brooks and W. Bruce Cox; Notice of Application for Transfer of License and Soliciting Comments and Motions To Intervene January 12, 2010. On October 30, 2009, J&T Hydro Company (transferor) and...

  11. Twenty-year inter-annual trends and seasonal variations in precipitation and stream water chemistry at the Bear Brook Watershed in Maine, USA

    Czech Academy of Sciences Publication Activity Database

    Navrátil, Tomáš; Norton, S. A.; Fernandez, I. J.; Nelson, S. J.

    2010-01-01

    Roč. 171, 1/4 (2010), s. 23-45 ISSN 0167-6369 Institutional research plan: CEZ:AV0Z30130516 Keywords : Bear Brook Watershed in Maine * precipitation chemistry * stream chemistry * seasonality * long-term trends Subject RIV: DD - Geochemistry Impact factor: 1.436, year: 2010

  12. Assessment of aquifer properties, evapotranspiration, and the effects of ditching in the Stoney Brook watershed, Fond du Lac Reservation, Minnesota, 2006-9

    Science.gov (United States)

    Jones, Perry M.; Tomasek, Abigail A.

    2015-01-01

    The U.S. Geological Survey, in cooperation with the Fond du Lac Band of Lake Superior Chippewa, assessed hydraulic properties of geologic material, recharge, and evapotranspiration, and the effects of ditching on the groundwater resources in the Stoney Brook watershed in the Fond du Lac Reservation. Geologic, groundwater, and surface-water data were collected during 2006–9 to estimate hydrologic properties in the watershed. Streamflow and groundwater levels in the shallow glacial deposits in the Stoney Brook watershed were analyzed to estimate groundwater-flow directions, groundwater recharge, and evapotranspiration within the watershed and to assess the effect of ditches on surrounding groundwater resources. Groundwater, streamflow, and precipitation data collected during the study (2006–9) can be used to update the U.S. Department of Agriculture’s Natural Resource Conservation Service and Fond du Lac Resource Management Division surface-water models, which are used to evaluate the effect of proposed adjustments to the ditching system on streamflow on wild rice production and aquatic habitats.

  13. Flood-Inundation maps for the Hohokus Brook in Waldwick Borough, Ho-Ho-Kus Borough, and the Village of Ridgewood, New Jersey, 2014

    Science.gov (United States)

    Watson, Kara M.; Niemoczynski, Michal J.

    2015-07-20

    Digital flood-inundation maps for a 6-mile reach of the Hohokus Brook in New Jersey from White's Lake Dam in Waldwick Borough, through Ho-Ho-Kus Borough to Grove Street in the Village of Ridgewood were created by the U.S. Geological Survey (USGS) in cooperation with the New Jersey Department of Environmental Protection. The flood inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage on the Hohokus Brook at Ho-Ho-Kus, New Jersey (station number 01391000). Stage data at this streamgage may be obtained on the Internet from the USGS National Water Information System at http://waterdata.usgs.gov/nwis/uv?site_no=01391000 or the National Weather Service (NWS) Advanced Hydrologic Prediction Service at http://water.weather.gov/ahps2/hydrograph.php?gage=hohn4&wfo=okx.

  14. Dry deposition of sulfur: a 23-year record for the Hubbard Brook Forest ecosystem

    Energy Technology Data Exchange (ETDEWEB)

    Likens, G E; Eaton, J S [Inst. of Ecosystem Studies, The New York Botanical Garden, NY (US); Bormann, F H [School of Forestry and Environmental Studies Yale Univ., New Haven, CT (US); Hedin, L O [Dept. of Biology, Yale Univ., New Haven, CT (US); Driscoll, C T [Dept. of Civil and Environmental Engineering, Syracuse, NY (US)

    1990-01-01

    Dry deposition of S was estimated for watershed-ecosystems of the Hubbard Brook Experimental Forest from 1964-65 through 1986-87. Two approaches, a regression analysis of bulk precipitation inputs and stream outputs and a mass-balance method, gave similar average values for Watershed 6 430 and 410 eq SO{sub 4}{sup =}/ha-yr, respectively, for this 23-year period. Dry deposition contributed about 37% of total S deposition, varying from 12% in 1964-65 to 61% in 1983-84. Long-term data from 'replicated' watershed-ecosystems showed that temporal variability in estimates of dry deposition was considerably greater than spatial (between watersheds) variability.

  15. Bull trout (Salvelinus confluentus) telemetry and associated habitat data collected in a geodatabase from the upper Boise River, southwestern Idaho

    Science.gov (United States)

    MacCoy, Dorene E.; Shephard, Zachary M.; Benjamin, Joseph R.; Vidergar, Dmitri T.; Prisciandaro, Anthony F.

    2017-03-23

    Bull trout (Salvelinus confluentus), listed as threatened under the Endangered Species Act, are among the more thermally sensitive of coldwater species in North America. The Boise River upstream of Arrowrock Dam in southwestern Idaho (including Arrowrock Reservoir) provides habitat for one of the southernmost populations of bull trout. The presence of the species in Arrowrock Reservoir poses implications for dam and reservoir operations. From 2011 to 2014, the Bureau of Reclamation and the U.S. Geological Survey collected fish telemetry data to improve understanding of bull trout distribution and movement in Arrowrock Reservoir and in the upper Boise River tributaries. The U.S. Geological Survey compiled the telemetry (fish location) data, along with reservoir elevation, river discharge, precipitation, and water-quality data in a geodatabase. The geodatabase includes metadata compliant with Federal Geographic Data Committee content standards. The Bureau of Reclamation plans to incorporate the data in a decision‑support tool for reservoir management.

  16. Twenty-year inter-annual trends and seasonal variations in precipitation and stream water chemistry at the Bear Brook Watershed in Maine, USA.

    Science.gov (United States)

    Navrátil, Tomas; Norton, Stephen A; Fernandez, Ivan J; Nelson, Sarah J

    2010-12-01

    Mean annual concentration of SO4(-2) in wet-only deposition has decreased between 1988 and 2006 at the paired watershed study at Bear Brook Watershed in Maine, USA (BBWM) due to substantially decreased emissions of SO(2). Emissions of NO(x) have not changed substantially, but deposition has declined slightly at BBWM. Base cations, NH4+, and Cl(-) concentrations were largely unchanged, with small irregular changes of Bear Brook (EB), the reference stream, has been slowly responding to reduced but still elevated acid deposition. Calcium and Mg have declined fairly steadily and faster than SO4(-2), with consequent acidification (lower pH and higher inorganic Al). Eighteen years of experimental treatment with (NH(4))(2)SO(4) enhanced acidification of West Bear Brook's (WB) watershed. Despite the manipulation, NH4+ concentration remained below detection limits at WB, while leaching of NO3- increased. The seasonal pattern for NO3- concentrations in WB, however, remained similar to EB. Mean monthly concentrations of SO4(-2) have increased in WB since 1989, initially only during periods of high flow, but gradually also during base flow. Increases in mean monthly concentrations of Ca(2+), Mg(2+), and K(+) due to the manipulation occurred from 1989 until about 1995, during the depletion of base cations in shallow flow paths in WB. Progressive depletion of Ca and Mg at greater soil depth occurred, causing stream concentrations to decline to pre-manipulation values. Mean monthly Si concentrations did not change in EB or WB, suggesting that the manipulation had no effect on mineral weathering rates. DOC concentrations in both streams did not exhibit inter- or intra-annual trends.

  17. Fish and crustaceans in northeast Greenland lakes with special emphasis on interactions between Arctic charr (Salvelinus alpinus), Lepidurus arcticus and benthic chydorids

    DEFF Research Database (Denmark)

    Jeppesen, E.; Christoffersen, K.; Landkildehus, F.

    2001-01-01

    We studied the trophic structure in the pelagial and crustacean remains in the surface 1 cm of the sediment of 13 shallow, high arctic lakes in northeast Greenland (74 N). Seven lakes were fishless, while the remaining six hosted a dwarf form of Arctic charr (Salvelinus alpinus). In fishless lakes...... sp. in lakes with Lepidurus, while they were abundant in lakes with fish. The low abundance in fishless lakes could not be explained by damage of crustacean remains caused by Lepidurus feeding in the sediment, because remains of the more soft-shelled, pelagic-living Daphnia were abundant...... in the sediment of these lakes. No significant differences between lakes with and without fish were found in chlorophyll a, total phosphorus, total nitrogen, conductivity or temperature, suggesting that the observed link between Lepidurus arcticus and the benthic crustacean community is causal. Consequently...

  18. Differences in organotin accumulation in relation to life history in the white-spotted charr Salvelinus leucomaenis

    International Nuclear Information System (INIS)

    Ohji, Madoka; Harino, Hiroya; Arai, Takaomi

    2011-01-01

    Research highlights: → Otolith Sr:Ca ratios in sea-run type were higher than those in freshwater-residents. → TBT and TPT concentrations in sea-run type were higher than those in freshwater-residents. → Sea-run type have higher risk of TBT and TPT than freshwater-residents in white-spotted charr. - Abstract: To examine the accumulation pattern of organotins (OTs) in relation to the migration of diadromous fish, tributyltin (TBT) and triphenyltin (TPT) and their derivatives were determined in the muscle tissue of both sea-run (anadromous) and freshwater-resident (nonanadromous) types of the white-spotted charr Salvelinus leucomaenis. Ontogenic changes in otolith strontium (Sr) and calcium (Ca) concentrations were examined along life history transect to discriminate migration type. Mean Sr:Ca ratio from the core to the edge of the otolith in sea-run individuals was significantly higher than those in freshwater-resident one. There were no significant correlations in S. leucomaenis between OT accumulation and various biological characteristics. It is noteworthy that TBT and TPT concentrations in sea-run type were significantly higher than those in freshwater-resident individuals, although they are both of the same species. These results suggest that sea-run S. leucomaenis have a higher ecological risk of OT exposure than freshwater-residents during their life histories.

  19. Use of the E. J. Brooks 'Multi-Lok' for material safeguards at the Y- 12 Plant

    International Nuclear Information System (INIS)

    Key, C.D.

    1995-01-01

    The Oak Ridge Y-12 Plant has begun using the E. J. Brooks 'Multi-Lok' as the replacement of the cup seal. The cup seal in previous years of usage has proved to be difficult to apply and verify, along with easily broken during handling. Replacement of the cup seal with the Multi-Lok has resulted in operations satisfaction in ease of application and verification. Inadvertent breakage of the previous fragile seal has been completely eliminated. Cost savings are abundant. The final result is customer satisfaction with optimum product performance

  20. Geomorphic, flood, and groundwater-flow characteristics of Bayfield Peninsula streams, Wisconsin, and implications for brook-trout habitat

    Science.gov (United States)

    Fitzpatrick, Faith A.; Peppler, Marie C.; Saad, David A.; Pratt, Dennis M.; Lenz, Bernard N.

    2015-01-01

    In 2002–03, the U.S. Geological Survey conducted a study of the geomorphic, flood, and groundwater-flow characteristics of five Bayfield Peninsula streams, Wisconsin (Cranberry River, Bark River, Raspberry River, Sioux River, and Whittlesey Creek) to determine the physical limitations for brook-trout habitat. The goals of the study were threefold: (1) to describe geomorphic characteristics and processes, (2) to determine how land-cover characteristics affect flood peaks, and (3) to determine how regional groundwater flow patterns affect base flow.

  1. THEOLOGY AS CREATIVE LANGUAGE. THE CASE OF THOMAS BROOKS

    Directory of Open Access Journals (Sweden)

    Ciprian SIMUȚ

    2015-12-01

    Full Text Available The problem of theological language has been a hindrance in coming closer to God, ever since complex patterns of thought have combined with the theological inquiry. The conclusion that helped Protestantism gain ground was bringing the theological message of the Bible to the understanding of every-day believer. An effort was made to explain how God works in history, and how to become more like Christ in one’s every-day life. However, mere copying was not the foundation of Christianity. Inner conviction born out of understanding the explanation from the Biblical message was crucial. Thomas Brooks was one of the protestant theologians who aimed to explain how the Devil and evil work against the believer, in order to create chaos, disorder, sorrow, hatred, deception, and ignorance. He did this by transforming the theological message into creative language, using every-day images in order to create a relation between theology and praxis. This paper will analyze some of the aspects found in Brooks’ theological presentation, in order to show how theology could be used for the benefit of the individual believer, as well as for the benefit of society.

  2. Beam emittance of the Stony Brook Tandem-LINAC booster

    International Nuclear Information System (INIS)

    Scholldorf, A.H.

    1984-01-01

    This dissertation is primarily a study of the longitudinal and transverse beam emittance of the Stony Brook Heavy Ion Tandem LINAC Accelerator Facility, with a secondary emphasis on the beam dynamical design of two key elements of the system: a low energy double-drift buncher, and an achromatic double-90 0 LINAC injection system. A transverse emittance measuring system consisting of two translation stages controlled by stepper motors is described. Each stage carried a pair of beam defining slits mounted so that both horizontal and vertical emittances could be measured with only linear motion of the stage assembly. Beam currents were measured directly by a low-noise, high-sensitivity electrometer circuit integrated with the second slit-stage assembly. A mini-computer controlled the motors and acquired and displayed the data. Transverse emittance areas of beams of 12 C, 16 O, 32 S, and 58 Ni were measured at ion source extraction potential, after ion source acceleration, after tandem acceleration, and after LINAC acceleration. The results were analyzed in terms of source sputter-cone geometry, angle straggling in gas and foil strippers, and a variety of other factors

  3. Simulation of groundwater flow and streamflow depletion in the Branch Brook, Merriland River, and parts of the Mousam River watersheds in southern Maine

    Science.gov (United States)

    Nielsen, Martha G.; Locke, Daniel B.

    2015-01-01

    Watersheds of three streams, the Mousam River, Branch Brook, and Merriland River in southeastern Maine were investigated from 2010 through 2013 under a cooperative project between the U.S. Geological Survey and the Maine Geological Survey. The Branch Brook watershed previously had been deemed “at risk” by the Maine Geological Survey because of the proportionally large water withdrawals compared to estimates of the in-stream flow requirements for habitat protection. The primary groundwater withdrawals in the study area include a water-supply well in the headwaters of the system and three water-supply wells in the coastal plain near the downstream end of the system. A steady-state groundwater flow model was used to understand the movement of water within the system, to evaluate the water budget and the effect of groundwater withdrawals on streamflows, and to understand streamflow depletion in relation to the State of Maine’s requirements to maintain in-stream flows for habitat protection.

  4. Extensive feeding on sockeye salmon Oncorhynchus nerka smolts by bull trout Salvelinus confluentus during initial outmigration into a small, unregulated and inland British Columbia river

    Science.gov (United States)

    Furey, Nathan B.; Hinch, Scott G.; Lotto, A.G.; Beauchamp, David A.

    2015-01-01

    Stomach contents were collected and analysed from 22 bull trout Salvelinus confluentus at the edge of the Chilko Lake and Chilko River in British Columbia, Canada, during spring outmigration of sockeye salmon Oncorhynchus nerka smolts. Twenty of the 22 (>90%) stomachs contained prey items, virtually all identifiable prey items were outmigrant O. nerka smolts and stomach contents represented a large portion (0·0–12·6%) of estimated S. confluentus mass. The results demonstrate nearly exclusive and intense feeding by S. confluentus on outmigrant smolts, and support recent telemetry observations of high disappearance rates of O. nerka smolts leaving large natural lake systems prior to entering high-order unregulated river systems.

  5. A Study of Fish Lice (Argulus Sp. Infection in Freshwater Food Fish

    Directory of Open Access Journals (Sweden)

    Aalberg K.

    2016-09-01

    Full Text Available Argulus sp., commonly referred to as fish lice, are crustacean ectoparasites of fishes. The hematophagous parasites attach to and feed off the integument of their hosts. Outbreaks of epizootics have been reported worldwide, causing mass mortalities and having serious economic implications for fish farms and culture efforts. Argulus fish lice may also serve as vectors of infectious diseases and as intermediate hosts of other parasites. Two native European species, A. foliaceus and A. coregoni, as well as the invasive Japanese fish louse A. japonicus, have previously been recorded in Slovakia. This study investigated samples collected at fish farms and culture sites of Common carp (Cyprinus carpio L., Pike-perch (Sander lucioperca L. and Brook trout (Salvelinus fontinalis M. in Eastern Slovakia, as well as samples collected from live fish imported to the Slovak Republic. A quantitative description of the of Argulus sp. was recorded from each locality. Samples from Common carp were identified as the invasive A. japonicus, and samples from Pike-perch and Brook trout were identified as A. foliaceus. Evidence of a mixed infection of Pike-perch with both A. foliaceus and A. japonicus was found in samples from Zemplínska Šírava, which was substantiated by electron microscopic examination. Morphometric characteristics were measured and averages and ranges produced for each species and sex.

  6. Soil bacterial communities of a calcium-supplemented and a reference watershed at the Hubbard Brook Experimental Forest (HBEF), New Hampshire, USA

    Science.gov (United States)

    Ganapathi Sridevi; Rakesh Minocha; Swathi A. Turlapati; Katherine C. Goldfarb; Eoin L. Brodie; Louis S. Tisa; Subhash C. Minocha

    2012-01-01

    Soil Ca depletion because of acidic deposition-related soil chemistry changes has led to the decline of forest productivity and carbon sequestration in the northeastern USA. In 1999, acidic watershed (WS) 1 at the Hubbard Brook Experimental Forest (HBEF), NH, USA was amended with Ca silicate to restore soil Ca pools. In 2006, soil samples were collected from the Ca-...

  7. State University of New York, University of Stoney Brook, University and Clinical Practice Management Plan Space Leasing Practices. Report 96-S-36.

    Science.gov (United States)

    New York State Office of the Comptroller, Albany. Div. of Management Audit.

    This audit report assesses the propriety and economy of space leasing practices of the State University of New York at Stony Brook (SUNY-SB) for the period July 1, 1994 through December 31, 1996, specifically those related to a health center that includes five professional schools, a 536-bed teaching hospital, and a 350-bed veterans' home. Some of…

  8. Do predators influence the distribution of age-0 kokanee in a Colorado Reservoir?

    Science.gov (United States)

    Hardiman, J.M.; Johnson, B.M.; Martinez, P.J.

    2004-01-01

    Seasonal changes in reservoir conditions such as productivity, light, and temperature create spatiotemporal variation in habitat that may segregate or aggregate predators and prey, producing implications for the distribution, growth, and survival of fishes. We used hydroacoustics to document the diel vertical distribution of age-0 kokanee Oncorhynchus nerka relative to environmental gradients at Blue Mesa Reservoir, Colorado, during May-August of 2002. Temperature, light, and zooplankton density profiles were examined relative to foraging conditions for kokanee and their primary predator, lake trout Salvelinus namaycush. Age-0 kokanee displayed large diel vertical migrations in May despite the lack of an energetic advantage before reservoir stratification. Age-0 kokanee minimized near-surface foraging at this time, perhaps to avoid predation by visual predators, such as lake trout, in the well-lit surface waters. Strong reservoir stratification in midsummer appeared to provide a thermal refuge from lake trout that the kokanee exploited. By August vertical migrations were shallow and most kokanee remained in the epilimnion throughout the day. Although the energetic implications of the late-summer strategy are unclear, it appears that kokanee were responding to changes in their predator environment. A robust model for kokanee diel vertical migration across a range of systems should include a predator avoidance component.

  9. Challenge to the model of lake charr evolution: Shallow- and deep-water morphs exist within a small postglacial lake

    Science.gov (United States)

    Chavarie, Louise; Muir, Andrew M.; Zimmerman, Mara S.; Baillie, Shauna M.; Hansen, Michael J.; Nate, Nancy A.; Yule, Daniel L.; Middel, Trevor; Bentzen, Paul; Krueger, Charles C.

    2016-01-01

    All examples of lake charr (Salvelinus namaycush) diversity occur within the largest, deepest lakes of North America (i.e. > 2000 km2). We report here Rush Lake (1.3 km2) as the first example of a small lake with two lake charr morphs (lean and huronicus). Morphology, diet, life history, and genetics were examined to demonstrate the existence of morphs and determine the potential influence of evolutionary processes that led to their formation or maintenance. Results showed that the huronicus morph, caught in deep-water, had a deeper body, smaller head and jaws, higher eye position, greater buoyancy, and deeper peduncle than the shallow-water lean morph. Huronicus grew slower to a smaller adult size, and had an older mean age than the lean morph. Genetic comparisons showed low genetic divergence between morphs, indicating incomplete reproductive isolation. Phenotypic plasticity and differences in habitat use between deep and shallow waters associated with variation in foraging opportunities seems to have been sufficient to maintain the two morphs, demonstrating their important roles in resource polymorphism. Rush Lake expands previous explanations for lake charr intraspecific diversity, from large to small lakes and from reproductive isolation to the presence of gene flow associated with strong ecological drivers.

  10. Angler-caught piscivore diets reflect fish community changes in Lake Huron

    Science.gov (United States)

    Roseman, Edward F.; Schaeffer, Jeff; Bright, Ethan; Fielder, David G.

    2014-01-01

    Examination of angler-caught piscivore stomachs revealed that Lake Trout Salvelinus namaycush, Chinook Salmon Oncorhynchus tshawytscha, and Walleyes Sander vitreus altered theirdiets in response to unprecedented declines in Lake Huron's main-basin prey fish community.Diets varied by predator species, season, and location but were nearly always dominated numerically by some combination of Alewife Alosa pseudoharengus, Rainbow Smelt Osmerus mordax, Emerald Shiner Notropis atherinoides, Round Goby Neogobius melanostomus, or terrestrial insects. Rainbow Trout Oncorhynchus mykiss (steelhead), Coho Salmon Oncorhynchus kisutch, and Atlantic Salmon Salmo salar had varied diets that reflected higher contributions of insects. Compared with an earlier (1983–1986) examination of angler-caught predator fishes from Lake Huron, the contemporary results showed an increase in consumption of nontraditional prey (including conspecifics), use of smaller prey, and an increase in insects in the diet, suggesting that piscivores were faced with chronic prey limitation during this study. The management of all piscivores in Lake Huron will likely require consideration of the pervasive effects of changes in food webs, especially if prey fish remain at low levels.

  11. Assessing Wetland Hydroperiod and Soil Moisture With Remote Sensing: A Demonstration for the NASA Plum Brook Station Year 2

    Science.gov (United States)

    Brooks, Colin; Bourgeau-Chavez, Laura; Endres, Sarah; Battaglia, Michael; Shuchman, Robert

    2015-01-01

    Primary Goal: Assist with the evaluation and measuring of wetlands hydroperiod at the PlumBrook Station using multi-source remote sensing data as part of a larger effort on projecting climate change-related impacts on the station's wetland ecosystems. MTRI expanded on the multi-source remote sensing capabilities to help estimate and measure hydroperiod and the relative soil moisture of wetlands at NASA's Plum Brook Station. Multi-source remote sensing capabilities are useful in estimating and measuring hydroperiod and relative soil moisture of wetlands. This is important as a changing regional climate has several potential risks for wetland ecosystem function. The year two analysis built on the first year of the project by acquiring and analyzing remote sensing data for additional dates and types of imagery, combined with focused field work. Five deliverables were planned and completed: 1) Show the relative length of hydroperiod using available remote sensing datasets 2) Date linked table of wetlands extent over time for all feasible non-forested wetlands 3) Utilize LIDAR data to measure topographic height above sea level of all wetlands, wetland to catchment area radio, slope of wetlands, and other useful variables 4) A demonstration of how analyzed results from multiple remote sensing data sources can help with wetlands vulnerability assessment 5) A MTRI style report summarizing year 2 results. This report serves as a descriptive summary of our completion of these our deliverables. Additionally, two formal meetings were held with Larry Liou and Amanda Sprinzl to provide project updates and receive direction on outputs. These were held on 2/26/15 and 9/17/15 at the Plum Brook Station. Principal Component Analysis (PCA) is a multivariate statistical technique used to identify dominant spatial and temporal backscatter signatures. PCA reduces the information contained in the temporal dataset to the first few new Principal Component (PC) images. Some advantages of PCA

  12. Soilaluminum, iron, and phosphorus dynamics in response to long-term experimental nitrogen and sulfur additions at the Bear Brook Watershed in Maine, USA

    Science.gov (United States)

    Jessica Sherman; Ivan J. Fernandez; Stephen A. Norton; Tsutomu Ohno; Lindsey E. Rustad

    2006-01-01

    Atmospheric deposition of nitrogen (N) and sulfur (S) containing compounds affects soil chemistry in forested ecosystems through (1) acidification and the depletion of base cations, (2) metal mobilization, particularly aluminum (Al), and iron (Fe), (3) phosphorus (P) mobilization, and (4) N accumulation. The Bear BrookWatershed in Maine (BBWM) is a long-term paired...

  13. Long-term calcium addition increases growth release, wound closure, and health of sugar maple (Acer saccharum) trees at the Hubbard Brook Experimental Forest

    Science.gov (United States)

    Brett A. Huggett; Paul G. Schaberg; Gary J. Hawley; Christopher Eager

    2007-01-01

    We surveyed and wounded forest-grown sugar maple (Acer saccharum Marsh.) trees in a long-term, replicated Ca manipulation study at the Hubbard Brook Experimental Forest in New Hampshire, USA. Plots received applications of Ca (to boost Ca availability above depleted ambient levels) or A1 (to compete with Ca uptake and further reduce Ca availability...

  14. ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA U.S. EPA DEMONSTRATION PROJECT AT SPRING BROOK MOBILE HOME PARK IN WALES, ME SIX-MONTH EVALUATION REPORT

    Science.gov (United States)

    This report documents the activities performed during and the results obtained from the first six months of the arsenic removal treatment technology demonstration project at the Spring Brook Mobile Home Park in Wales, ME. The objectives of the project are to evaluate the effectiv...

  15. Biochemical characterization of the Arctic char (Salvelinus alpinus ovarian progestin membrane receptor

    Directory of Open Access Journals (Sweden)

    Thomas Peter

    2005-11-01

    Full Text Available Abstract Membrane progestin receptors are involved in oocyte maturation in teleosts. However, the maturation-inducing steroid (MIS does not appear to be conserved among species and several progestins may fulfill this function. So far, complete biochemical characterization has only been performed on a few species. In the present study we have characterized the membrane progestin receptor in Arctic char (Salvelinus alpinus and show that the 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P receptor also binds several xenobiotics, thus rendering oocyte maturation sensitive to environmental pollutants. We identified a single class of high affinity (Kd, 13.8 ± 1.1 nM, low capacity (Bmax, 1.6 ± 0.6 pmol/g ovary binding sites by saturation and Scatchard analyses. Receptor binding displayed rapid association and dissociation kinetics typical of steroid membrane receptors, with t1/2 s of less than 1 minute. The 17,20beta-P binding also displayed tissue specificity with high, saturable, and specific 17,20beta-P binding detected in ovaries, heart and gills while no specific binding was observed in muscle, brain or liver. Changes in 17,20beta-P binding during oocyte maturation were consistent with its identity as the oocyte MIS membrane receptor. Incubation of fully-grown ovarian follicles with gonadotropin induced oocyte maturation, which was accompanied by a five-fold increase in 17,20beta-P receptor binding. In addition, competition studies with a variety of steroids revealed that receptor binding is highly specific for 17,20beta-P, the likely maturation-inducing steroid (MIS in Arctic char. The relative-binding affinities of all the other progestogens and steroids tested were less than 5% of that of 17,20beta-P for the receptor. Several ortho, para derivatives of DDT also showed weak binding affinity for the 17,20beta-P receptor supporting the hypothesis that xenobiotics may bind steroid receptors on the oocyte's surface and might thereby interfere

  16. Provenance and detrital zircon geochronologic evolution of lower Brookian foreland basin deposits of the western Brooks Range, Alaska, and implications for early Brookian tectonism

    Science.gov (United States)

    Moore, Thomas; O'Sullivan, Paul B.; Potter, Christopher J.; Donelick, Raymond A.

    2015-01-01

    The Upper Jurassic and Lower Cretaceous part of the Brookian sequence of northern Alaska consists of syntectonic deposits shed from the north-directed, early Brookian orogenic belt. We employ sandstone petrography, detrital zircon U-Pb age analysis, and zircon fission-track double-dating methods to investigate these deposits in a succession of thin regional thrust sheets in the western Brooks Range and in the adjacent Colville foreland basin to determine sediment provenance, sedimentary dispersal patterns, and to reconstruct the evolution of the Brookian orogen. The oldest and structurally highest deposits are allochthonous Upper Jurassic volcanic arc–derived sandstones that rest on accreted ophiolitic and/or subduction assemblage mafic igneous rocks. These strata contain a nearly unimodal Late Jurassic zircon population and are interpreted to be a fragment of a forearc basin that was emplaced onto the Brooks Range during arc-continent collision. Synorogenic deposits found at structurally lower levels contain decreasing amounts of ophiolite and arc debris, Jurassic zircons, and increasing amounts of continentally derived sedimentary detritus accompanied by broadly distributed late Paleozoic and Triassic (359–200 Ma), early Paleozoic (542–359 Ma), and Paleoproterozoic (2000–1750 Ma) zircon populations. The zircon populations display fission-track evidence of cooling during the Brookian event and evidence of an earlier episode of cooling in the late Paleozoic and Triassic. Surprisingly, there is little evidence for erosion of the continental basement of Arctic Alaska, its Paleozoic sedimentary cover, or its hinterland metamorphic rocks in early foreland basin strata at any structural and/or stratigraphic level in the western Brooks Range. Detritus from exhumation of these sources did not arrive in the foreland basin until the middle or late Albian in the central part of the Colville Basin.These observations indicate that two primary provenance areas provided

  17. Evaluation of glutamic acid and glycine as sources of nonessential amino acids for lake trout (Salvelinus namaycush) and rainbow trout (Salmo gairdnerii)

    Science.gov (United States)

    Hughes, S.G.

    1985-01-01

    1. A semi-purified test diet which contained either glutamic acid or glycine as the major source of nonessential amino acids (NEAA) was fed to lake and rainbow trout.2. Trout fed the diet containing glutamic acid consistently showed better growth and feed conversion efficiencies than those fed the diets containing glycine.3. The data indicate that these trout utilize glutamic acid more efficiently than glycine when no other major sources of NEAA are present.

  18. Alien invasions in aquatic ecosystems: toward an understanding of brook trout invasions and potential impacts on inland cutthroat trout in western North America

    Science.gov (United States)

    Jason B. Dunham; Susan B. Adams; Robert E. Schroeter; Douglas C. Novinger

    2002-01-01

    Experience from case studies of biological invasions in aquatic ecosystems has motivated a set of proposed empirical “rules” for understanding patterns of invasion and impacts on native species. Further evidence is needed to better understand these patterns, and perhaps contribute to a useful predictive theory of invasions. We reviewed the case of brook trout (

  19. Results of the radiological survey at Interstate 80, North Right of Way at Lodi Brook, Lodi, New Jersey (LJ077)

    International Nuclear Information System (INIS)

    Foley, R.D.; Floyd, L.M.

    1989-06-01

    Maywood Chemical Works (MCW) of Maywood, New Jersey, generated process wastes and residues associated with the production and refining of thorium and thorium compounds from monazite ores from 1916 to 1956. MCW supplied rare earth metals and thorium compounds to the Atomic Energy Commission and various other government agencies from the late 1940s to the mid-1950s. Area residents used the sandlike waste from this thorium extraction process mixed with tea and cocoa leaves as mulch in their yards. Some of these contaminated wastes were also eroded from the site into Lodi Brook. At the request of the US Department of Energy (DOE), a group from Oak Ridge National Laboratory conducts investigative radiological surveys of properties in the vicinity of MCW to determine whether a property is contaminated with radioactive residues, principally 232 Th, derived from the MCW site. The survey typically includes direct measurement of gamma radiation levels and oil sampling for radionuclide analyses. The survey of this site, on the North Right of Way of Interstate 80 at Lodi Brook, Lodi, New Jersey (LJ077), was conducted during 1988. Results of the survey demonstrated radionuclide concentrations in excess of the DOE Formerly Utilized Sites Remedial Action Program criteria. The radionuclide distributions are typical of the type of material originating from the MCW site. 5 refs., 3 figs., 3 tabs

  20. Marcellus and mercury: Assessing potential impacts of unconventional natural gas extraction on aquatic ecosystems in northwestern Pennsylvania.

    Science.gov (United States)

    Grant, Christopher J; Weimer, Alexander B; Marks, Nicole K; Perow, Elliott S; Oster, Jacob M; Brubaker, Kristen M; Trexler, Ryan V; Solomon, Caroline M; Lamendella, Regina

    2015-01-01

    Mercury (Hg) is a persistent element in the environment that has the ability to bioaccumulate and biomagnify up the food chain with potentially harmful effects on ecosystems and human health. Twenty-four streams remotely located in forested watersheds in northwestern PA containing naturally reproducing Salvelinus fontinalis (brook trout), were targeted to gain a better understanding of how Marcellus shale natural gas exploration may be impacting water quality, aquatic biodiversity, and Hg bioaccumulation in aquatic ecosystems. During the summer of 2012, stream water, stream bed sediments, aquatic mosses, macroinvertebrates, crayfish, brook trout, and microbial samples were collected. All streams either had experienced hydraulic fracturing (fracked, n = 14) or not yet experienced hydraulic fracturing (non-fracked, n = 10) within their watersheds at the time of sampling. Analysis of watershed characteristics (GIS) for fracked vs non-fracked sites showed no significant differences (P > 0.05), justifying comparisons between groups. Results showed significantly higher dissolved total mercury (FTHg) in stream water (P = 0.007), lower pH (P = 0.033), and higher dissolved organic matter (P = 0.001) at fracked sites. Total mercury (THg) concentrations in crayfish (P = 0.01), macroinvertebrates (P = 0.089), and predatory macroinvertebrates (P = 0.039) were observed to be higher for fracked sites. A number of positive correlations between amount of well pads within a watershed and THg in crayfish (r = 0.76, P shale natural gas exploration is having an effect on aquatic ecosystems.

  1. Phylogeographical analysis reveals multiple conservation units in brook lampreys Lampetra planeri of Portuguese streams.

    Science.gov (United States)

    Pereira, A M; Robalo, J I; Freyhof, J; Maia, C; Fonseca, J P; Valente, A; Almada, V C

    2010-08-01

    The populations of brook lamprey Lampetra planeri of Portuguese Rivers were analysed phylogeographically using a fragment of 644 bp of the mitochondrial control region of 158 individuals from six populations. Samples representing L. planeri and migratory lampreys Lampetra fluviatilis of rivers draining to the North Sea and the Baltic Sea were also included to assess the relationships of Portuguese samples. The data support a clear differentiation of all the populations studied. Several populations, which are isolated among themselves and also from the migratory lampreys, proved to be entirely composed of private haplotypes, a finding that supports some time of independent evolutionary history for these populations. This, combined with the geographic confinement to small water bodies, justifies the recognition of at least four conservation units in the Portuguese rivers Sado, São Pedro, Nabão and Inha.

  2. Temperature profile and water depth data collected from BROOKE using BT and XBT casts in the North Pacific Ocean from 03 October 1975 to 18 November 1977 (NODC Accession 8900225)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the BROOKE in the North Pacific Ocean and TOGA Area - Pacific Ocean. Data were...

  3. Can personality predict individual differences in brook trout spatial learning ability?

    Science.gov (United States)

    White, S.L.; Wagner, Tyler; Gowan, C.; Braithwaite, V.A.

    2017-01-01

    While differences in individual personality are common in animal populations, understanding the ecological significance of variation has not yet been resolved. Evidence suggests that personality may influence learning and memory; a finding that could improve our understanding of the evolutionary processes that produce and maintain intraspecific behavioural heterogeneity. Here, we tested whether boldness, the most studied personality trait in fish, could predict learning ability in brook trout. After quantifying boldness, fish were trained to find a hidden food patch in a maze environment. Stable landmark cues were provided to indicate the location of food and, at the conclusion of training, cues were rearranged to test for learning. There was a negative relationship between boldness and learning as shy fish were increasingly more successful at navigating the maze and locating food during training trials compared to bold fish. In the altered testing environment, only shy fish continued using cues to search for food. Overall, the learning rate of bold fish was found to be lower than that of shy fish for several metrics suggesting that personality could have widespread effects on behaviour. Because learning can increase plasticity to environmental change, these results have significant implications for fish conservation.

  4. Analysis of reinjection problems at the Stony Brook ATES field test site

    Science.gov (United States)

    Supkow, D. J.; Shultz, J. A.

    1982-12-01

    Aquifer Thermal Energy Storage (ATES) is one of several energy storage technologies being investigated by the DOE to determine the feasibility of reducing energy consumption by means of energy management systems. The State University of New York, (SUNY) Stony Brook, Long Island, New York site was selected by Battelle PNL for a Phase 1 investigation to determine the feasibility of an ATES demonstration to seasonally store chill energy by injecting chilled water in the winter and recovering it at a maximum rate of 100 MBTU/hr (30 MW) in the summer. The Phase 1 study was performed during 1981 by Dames & Moore under subcontract to Batelle PLN. The pumping and injection tests were performed using two wells in a doublet configuration. Well PI-1 is a previously existing well and PI-2 was installed specifically for this investigation. Both wells are screened in the Upper Magothy aquifer from approximately 300 to 350 feet below ground surface. Nine observation wells were also installed as a portion of the investigation to monitor water level and aquifer temperature changes during the test.

  5. Radiocaesium turnover in Arctic charr (Salvelinus alpinus) and brown trout (Salmo trutta) in a Norwegian lake

    International Nuclear Information System (INIS)

    Forseth, T.; Ugedal, O.; Jonsson, B.; Langeland, A.; Njaastad, O.

    1991-01-01

    The radioactivity of brown trout (Salmo trutta L.) and Arctic charr (Salvelinus alpinus (L.)) was monitored in a Norwegian lake from 1986 to 1989. A distinct difference was observed between brown trout and Arctic charr in the accumulation of radiocaesium ( 134 Cs and 137 Cs) from the Chernobyl fallout, and the study focused on the understanding of this difference. Brown trout had a large food consumption and a corresponding high intake of radiocaesium. Excretion was 20% faster in brown trout than Arctic charr as brown trout lived at high temperatures in epilimnic water. Arctic charr had a lower food consumption (less than one-third of trout) and lived in colder meta-and hypolimnic water. Arctic charr therefore had a lower intake and slower excretion of radiocaesium. Brown trout an Arctic charr had different diets. For brown trout zoobenthos was the dominant food item, whereas Artic charr mainly fed on zooplankton. The radioactivity in the stomach contents of the two species was different in 1986, but similar for the rest of the period. Higher levels of radiocaesium in brown trout than Arctic charr in 1986 were due to a higher food consumption and more radioactive food items in its diet. The parallel development in accumulated radiocaesium through summer 1987 was probably formed by brown trout balancing a higher intake with a faster excretion. The ecological half-lives of radiocaesium in brown trout (357 days) and Arctic charr (550 days) from Lake Hoeysjoeen indicated a slow removal of the isotopes from the food webs. (author)

  6. Visual sensitivity of deepwater fishes in Lake Superior.

    Directory of Open Access Journals (Sweden)

    Kelly A Harrington

    Full Text Available The predator-prey interactions in the offshore food web of Lake Superior have been well documented, but the sensory systems mediating these interactions remain unknown. The deepwater sculpin, (Myoxocephalus thompsoni, siscowet (Salvelinus namaycush siscowet, and kiyi (Coregonus kiyi inhabit low light level environments. To investigate the potential role of vision in predator-prey interactions, electroretinography was used to determine visual sensitivity for each species. Spectral sensitivity curves revealed peak sensitivity at 525 nm for each species which closely corresponds to the prevalent downwelling light spectrum at depth. To determine if sufficient light was available to mediate predator-prey interactions, visual sensitivity was correlated with the intensity of downwelling light in Lake Superior to construct visual depth profiles for each species. Sufficient daytime irradiance exists for visual interactions to approximately 325 m for siscowet and kiyi and 355 m for the deepwater sculpin during summer months. Under full moon conditions, sufficient irradiance exists to elicit ERG response to light available at approximately 30 m for the siscowet and kiyi and 45 m for the deepwater sculpin. Visual interactions are therefore possible at the depths and times when these organisms overlap in the water column indicating that vision may play a far greater role at depth in deep freshwater lakes than had been previously documented.

  7. Factors Affecting Mercury Stable Isotopic Distribution in Piscivorous Fish of the Laurentian Great Lakes.

    Science.gov (United States)

    Lepak, Ryan F; Janssen, Sarah E; Yin, Runsheng; Krabbenhoft, David P; Ogorek, Jacob M; DeWild, John F; Tate, Michael T; Holsen, Thomas M; Hurley, James P

    2018-03-06

    Identifying the sources of methylmercury (MeHg) and tracing the transformations of mercury (Hg) in the aquatic food web are important components of effective strategies for managing current and legacy Hg sources. In our previous work, we measured stable isotopes of Hg (δ 202 Hg, Δ 199 Hg, and Δ 200 Hg) in the Laurentian Great Lakes and estimated source contributions of Hg to bottom sediment. Here, we identify isotopically distinct Hg signatures for Great Lakes trout ( Salvelinus namaycush) and walleye ( Sander vitreus), driven by both food-web and water-quality characteristics. Fish contain high values for odd-isotope mass independent fractionation (MIF) with averages ranging from 2.50 (western Lake Erie) to 6.18‰ (Lake Superior) in Δ 199 Hg. The large range in odd-MIF reflects variability in the depth of the euphotic zone, where Hg is most likely incorporated into the food web. Even-isotope MIF (Δ 200 Hg), a potential tracer for Hg from precipitation, appears both disconnected from lake sedimentary sources and comparable in fish among the five lakes. We suggest that similar to the open ocean, water-column methylation also occurs in the Great Lakes, possibly transforming recently deposited atmospheric Hg deposition. We conclude that the degree of photochemical processing of Hg is controlled by phytoplankton uptake rather than by dissolved organic carbon quantity among lakes.

  8. Level II scour analysis for Bridge 8 (BARTTH00020008) on Town Highway 2, crossing Roaring Brook, Barton, Vermont

    Science.gov (United States)

    Boehmler, Erick M.; Ivanoff, Michael A.

    1996-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure BARTTH00020008 on town highway 2 crossing Roaring Brook, Barton, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from VTAOT files, was compiled prior to conducting Level I and Level II analyses and can be found in Appendix D. The site is in the New England Upland section of the New England physiographic province of North-central Vermont in the town of Barton. The 9.89-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the banks have woody vegetation coverage except for the downstream left bank, which has a few trees and grass and brush coverage. In the study area, Roaring Brook has an incised, sinuous channel with a slope of approximately 0.019 ft/ft, an average channel top width of 35 ft and an average channel depth of 3 ft. The predominant channel bed material is gravel/cobble (D50 is 49.1 mm or 0.161 ft). The geomorphic assessment at the time of the Level I and Level II site visit on October 18, 1994 indicated that the reach was laterally unstable. A cut-bank on the downstream right bank and overall channel configuration in the valley are indications of the lateral instability at this site. The town highway 2 crossing of Roaring Brook is a 30-ft-long, two-lane bridge consisting of one 26-foot span concrete T-beam type superstructure (Vermont Agency of Transportation, written communication, August 4, 1994). The bridge is supported by vertical, concrete abutments. The channel is skewed approximately 15 degrees to the opening while the opening-skew-to-roadway is

  9. Can personality predict individual differences in brook trout spatial learning ability?

    Science.gov (United States)

    White, S L; Wagner, T; Gowan, C; Braithwaite, V A

    2017-08-01

    While differences in individual personality are common in animal populations, understanding the ecological significance of variation has not yet been resolved. Evidence suggests that personality may influence learning and memory; a finding that could improve our understanding of the evolutionary processes that produce and maintain intraspecific behavioural heterogeneity. Here, we tested whether boldness, the most studied personality trait in fish, could predict learning ability in brook trout. After quantifying boldness, fish were trained to find a hidden food patch in a maze environment. Stable landmark cues were provided to indicate the location of food and, at the conclusion of training, cues were rearranged to test for learning. There was a negative relationship between boldness and learning as shy fish were increasingly more successful at navigating the maze and locating food during training trials compared to bold fish. In the altered testing environment, only shy fish continued using cues to search for food. Overall, the learning rate of bold fish was found to be lower than that of shy fish for several metrics suggesting that personality could have widespread effects on behaviour. Because learning can increase plasticity to environmental change, these results have significant implications for fish conservation. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Mercury speciation comparison. BrooksApplied Laboratories and Eurofins Frontier Global Sciences

    Energy Technology Data Exchange (ETDEWEB)

    Bannochie, C. J. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Wilmarth, W. R. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-12-16

    The Savannah River National Laboratory (SRNL) was tasked with preparing and shipping samples for Hg speciation by Eurofins Frontier Global Sciences (FGS), Inc. in Bothell, WA on behalf of the Savannah River Remediation (SRR) Mercury Program Team. These samples were analyzed for seven species including: total mercury, dissolved mercury, inorganic mercury ((Hg(I) and Hg(II)), elemental mercury, methylmercury, ethylmercury, and dimethylmercury, with an eighth species, particulate mercury, calculated from the difference between total and dissolved mercury after subtracting the elemental mercury. The species fraction of total mercury measured has ranged broadly from a low of 32% to a high of 146%, though the vast majority of samples have been <100%. This can be expected since one is summing multiple values that each have at least a ± 20% measurement uncertainty. Two liquid waste tanks particularly important to understanding the distribution of mercury species in the Savannah River Site (SRS) Tank Farm were selected for a round robin analysis by Eurofins FGS and BrooksApplied Laboratories (BAL). The analyses conducted by BAL on the Tank 22 and 38 samples and their agreement with those obtained from Eurofins FGS for total mercury, dissolved mercury, methylmercury, ethylmercury, and dimethylmercury provide a strong degree of confidence in these species measurements

  11. Mercury speciation comparison. BrooksApplied Laboratories and Eurofins Frontier Global Sciences

    International Nuclear Information System (INIS)

    Bannochie, C. J.; Wilmarth, W. R.

    2016-01-01

    The Savannah River National Laboratory (SRNL) was tasked with preparing and shipping samples for Hg speciation by Eurofins Frontier Global Sciences (FGS), Inc. in Bothell, WA on behalf of the Savannah River Remediation (SRR) Mercury Program Team. These samples were analyzed for seven species including: total mercury, dissolved mercury, inorganic mercury ((Hg(I) and Hg(II)), elemental mercury, methylmercury, ethylmercury, and dimethylmercury, with an eighth species, particulate mercury, calculated from the difference between total and dissolved mercury after subtracting the elemental mercury. The species fraction of total mercury measured has ranged broadly from a low of 32% to a high of 146%, though the vast majority of samples have been <100%. This can be expected since one is summing multiple values that each have at least a ± 20% measurement uncertainty. Two liquid waste tanks particularly important to understanding the distribution of mercury species in the Savannah River Site (SRS) Tank Farm were selected for a round robin analysis by Eurofins FGS and BrooksApplied Laboratories (BAL). The analyses conducted by BAL on the Tank 22 and 38 samples and their agreement with those obtained from Eurofins FGS for total mercury, dissolved mercury, methylmercury, ethylmercury, and dimethylmercury provide a strong degree of confidence in these species measurements

  12. Distribution of Po-210 and Pb-210 in Arctic Char (Salvelinus alpinus) from an Arctic freshwater lake

    Energy Technology Data Exchange (ETDEWEB)

    Gwynn, J.P.; Rudolfsen, G. [Norwegian Radiation Protection Authority, The Fram Centre, Tromsoe (Norway)

    2014-07-01

    There is little information available with regard to the accumulation of Po-210 and Pb-210 by freshwater fish in natural freshwater systems despite the potential for relevant ingestion doses to man. This is maybe of particular pertinence for certain population groups where freshwater fish are an important dietary food item. Equally, it is important to understand the body distributions of these naturally occurring radionuclides to quantify the resulting doses to different tissues and organs of freshwater fish. With regard to the latter, it is important to consider not only the doses arising from bio-accumulated Po-210 and Pb-210 in various body compartments but additionally the internal dose from unabsorbed Po-210 and Pb-210 in the digestive tract. In this study, activity concentrations of Po-210 and Pb-210 were determined in muscle and various internal organs of Arctic Charr (Salvelinus alpinus) sampled from a lake in the Norwegian Arctic (69 deg. 4' N, 19 deg. 20' E). Observed activity concentrations of Po-210 and Pb-210 in different tissues will be discussed in relation to physiological parameters and ambient lake water activity concentrations. Results from this study will be compared to two similar studies conducted in freshwater systems where elevated activity concentrations of these radionuclides have been observed. Ingestion dose rates to man and effective absorbed dose rates to different tissues and organs of Arctic Charr from Po-210 and Pb-210 will be derived and compared to those from observed activity concentrations of the anthropogenic radionuclide Cs-137. (authors)

  13. Level II scour analysis for Bridge 37 (TOWNTH00290037) on Town Highway 29, crossing Mill Brook, Townshend, Vermont

    Science.gov (United States)

    Burns, R.L.; Medalie, Laura

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure TOWNTH00290037 on Town Highway 29 crossing Mill Brook, Townshend, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D.

  14. Level II scour analysis for Bridge 8, (MANCTH00060008) on Town Highway 6, crossing Bourn Brook, Manchester, Vermont

    Science.gov (United States)

    Burns, Ronda L.; Hammond, Robert E.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure MANCTH00060008 on Town Highway 6 crossing Bourn Brook, Manchester, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.

  15. Level II scour analysis for Bridge 30, (HUNTTH00220030), on Town Highway 22, crossing Brush Brook, Huntington, Vermont

    Science.gov (United States)

    Burns, Ronda L.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure HUNTTH00220030 on Town Highway 22 crossing Brush Brook, Huntington, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.

  16. Level II scour analysis for brigde 5 (STOCTH00360005) on Town Highway 36, crossing Stony Brook, Stockridge, Vermont

    Science.gov (United States)

    Striker, Lora K.; Weber, Matthew A.

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure STOCTH00360005 on Town Highway 36 crossing Stony Brook, Stockbridge, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D.

  17. Level II scour analysis for Bridge 16, (NEWBTH00500016) on Town Highway 50, crossing Halls Brook, Newbury, Vermont

    Science.gov (United States)

    Burns, Ronda L.; Degnan, James R.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure NEWBTH00500016 on Town Highway 50 crossing Halls Brook, Newbury, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.

  18. Cities, Towns and Villages, City limit boundaries for all municipalities in Ben Hill, Brooks, Cook, Echols, Lanier, Irwin, Lowndes, Tift and Turner Counties., Published in 2010, 1:7200 (1in=600ft) scale, Southern Georgia Regional Commission.

    Data.gov (United States)

    NSGIC Regional | GIS Inventory — Cities, Towns and Villages dataset current as of 2010. City limit boundaries for all municipalities in Ben Hill, Brooks, Cook, Echols, Lanier, Irwin, Lowndes, Tift...

  19. Level II scour analysis for Bridge 37, (BRNETH00740037) on Town Highway 74, crossing South Peacham Brook, Barnet, Vermont

    Science.gov (United States)

    Burns, Ronda L.; Severance, Timothy

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure BRNETH00740037 on Town Highway 74 crossing South Peacham Brook, Barnet, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.

  20. Water quality trends in the Blackwater River watershed, West Virginia

    Science.gov (United States)

    Smith, Jessica; Welsh, Stuart A.; Anderson, James T.; Fortney, Ronald H.

    2015-01-01

    An understanding of historic and current water quality is needed to manage and improve aquatic communities within the Blackwater River watershed, WV. The Blackwater River, which historically offered an excellent Salvelinus fontinalis (Brook Trout) fishery, has been affected by logging, coal mining, use of off-road vehicles, and land development. Using information-theoretic methods, we examined trends in water quality at 12 sites in the watershed for the 14 years of 1980–1993. Except for Beaver Creek, downward trends in acidity and upward trends in alkalinity, conductivity, and hardness were consistent with decreases in hydrogen ion concentration. Water-quality trends for Beaver Creek were inconsistent with the other sites and reflect ongoing coal-mining influences. Dissolved oxygen trended downward, possibly due to natural conditions, but remained above thresholds that would be detrimental to aquatic life. Water quality changed only slightly within the watershed from 1980–1993, possibly reflecting few changes in development and land uses during this time. These data serve as a baseline for future water-quality studies and may help to inform management planning.

  1. Water striders (family Gerridae): mercury sentinels in small freshwater ecosystems

    International Nuclear Information System (INIS)

    Jardine, Timothy D.; Al, Tom A.; MacQuarrie, Kerry T.B.; Ritchie, Charles D.; Arp, Paul A.; Maprani, Antu; Cunjak, Richard A.

    2005-01-01

    To circumvent some of the previous limitations associated with contaminant-monitoring programs, we tested the suitability of the water strider (Hemiptera: Gerridae) as a mercury sentinel by comparing total mercury concentrations in water striders and brook trout (Salvelinus fontinalis) from a variety of stream sites in New Brunswick, Canada. There was a strong association between the two variables across sites (r 2 = 0.81, P < 0.001) in systems where both atmospheric deposition and a point source (an abandoned gold mine) were likely contributing to ambient mercury levels. In a small stream draining the gold mine tailings pile, water striders had mercury concentrations an order of magnitude higher than those from reference locations. Temporal variation at three southern New Brunswick stream sites was non-significant. These results suggest that water strider mercury levels accurately quantify food chain entry of the element. The use of sentinel species holds great potential for expanding contaminant-monitoring programs. - Water striders accurately reflect the entry of mercury in food chains of small freshwater systems

  2. NRDA-processed CTD data from the Brooks McCall in the Gulf of Mexico, Cruise 2 Leg 1, collected from 2010-05-15 to 2010-05-17, associated with the Deepwater Horizon Oil Spill event (NCEI Accession 0130011)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conductivity Temperature and Depth (CTD) measurements were collected aboard the R/V Brooks McCall, Cruise 02 to determine physical oceanographic parameters of the...

  3. NRDA-processed CTD data from the Brooks McCall in the Gulf of Mexico, Cruise 12 Leg 1, collected from 2010-07-11 to 2010-07-13, associated with the Deepwater Horizon Oil Spill event (NCEI Accession 0130009)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conductivity Temperature and Depth (CTD) measurements were collected aboard the R/V Brooks McCall, Cruise 12, to determine physical oceanographic parameters of the...

  4. NRDA-processed CTD data from the Brooks McCall in the Gulf of Mexico, Cruise 9 Leg 1, collected from 2010-06-23 to 2010-06-25, associated with the Deepwater Horizon Oil Spill event (NCEI Accession 0130015)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conductivity Temperature and Depth (CTD) measurements were collected aboard the R/V Brooks McCall, Cruise 09 to determine physical oceanographic parameters of the...

  5. NRDA-processed CTD data from the Brooks McCall in the Gulf of Mexico, Cruise 7 Leg 1, collected from 2010-06-11 to 2010-06-13, associated with the Deepwater Horizon Oil Spill event (NCEI Accession 0130013)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conductivity Temperature and Depth (CTD) measurements were collected aboard the R/V Brooks McCall, Cruise 07, to determine physical oceanographic parameters of the...

  6. NRDA-processed CTD data from the Brooks McCall in the Gulf of Mexico, Cruise 11 Leg 1, collected from 2010-07-05 to 2010-07-06, associated with the Deepwater Horizon Oil Spill event (NCEI Accession 0130008)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conductivity Temperature and Depth (CTD) measurements were collected aboard the R/V Brooks McCall, Cruise 11, to determine physical oceanographic parameters of the...

  7. Effectiveness of egg immersion in aqueous solutions of thiamine and thiamine analogs for reducing early mortality syndrome

    Science.gov (United States)

    Brown, S.B.; Brown, L.R.; Brown, M.; Moore, K.; Villella, M.; Fitzsimons, J.D.; Williston, B.; Honeyfield, D.C.; Hinterkopf, J.P.; Tillitt, D.E.; Zajicek, J.L.; Wolgamood, M.

    2005-01-01

    Protocols used for therapeutic thiamine treatments in salmonine early mortality syndrome (EMS) were investigated in lake trout Salvelinus namaycush and coho salmon Oncorhynchus kisutch to assess their efficacy. At least 500 mg of thiamine HCl/L added to egg baths was required to produce a sustained elevation of thiamine content in lake trout eggs. Thiamine uptake from egg baths was not influenced by a pH ranging from 5.5 to 7.5 or by a water hardness between 2 and 200 mg CaCO3/L. There was poorer thiamine uptake when initial thiamine levels were low, suggesting that current treatment regimes may not be as effective when thiamine levels are severely depressed and that higher treatment doses are necessary. Exposure of eggs to the more lipid-soluble thiamine analog allithiamine (1,000 mg/L) during water hardening increased egg thiamine levels by 1.5-2.5 nmol/g and was completely effective at reversing EMS. Another more lipid-soluble thiamine analog, benfotiamine (100 mg/L), reduced EMS but did not produce detectable increases in egg thiamine content. Although benfotiamine may be more effective than thiamine at mitigating EMS, it is more expensive than thiamine HCl or allithiamine. In addition, there still needs to be a more thorough examination of dose-response relationships. We conclude that allithiamine is an alternative to the use of thiamine in egg baths as a therapeutic treatment for salmonid EMS. ?? Copyright by the American Fisheries Society 2005.

  8. Seasonal patterns in growth, blood consumption, and effects on hosts by parasitic-phase sea lampreys in the Great Lakes: an individual-based model approach

    Science.gov (United States)

    Madenjian, Charles P.; Cochran, Philip A.; Bergstedt, Roger A.

    2003-01-01

    An individual-based model (IBM) was developed for sea lamprey (Petromyzon marinus) populations in the Laurentian Great Lakes. The IBM was then calibrated to observed growth, by season, for sea lampreys in northern Lake Huron under two different water temperature regimes: a regime experienced by Seneca-strain lake trout (Salvelinus namaycush) and a regime experienced by Marquettestrain lake trout. Modeling results indicated that seasonal blood consumption under the Seneca regime was very similar to that under the Marquette regime. Simulated mortality of lake trout directly due to blood removal by sea lampreys occurred at nearly twice the rate during August and September under the Marquette regime than under the Seneca regime. However, cumulative sea lamprey-induced mortality on lake trout over the entire duration of the sea lamprey's parasitic phase was only 7% higher for the Marquette regime compared with the Seneca regime. Thus, these modeling results indicated that the strain composition of the host (lake trout) population was not important in determining total number of lake trout deaths or total blood consumption attributable to the sea lamprey population, given the sea lamprey growth pattern. Regardless of water temperature regime, both blood consumption rate by sea lampreys and rate of sea lamprey-induced mortality on lake trout peaked in late October. Elevated blood consumption in late October appeared to be unrelated to changes in water temperature. The IBM approach should prove useful in optimizing control of sea lampreys in the Laurentian Great Lakes.

  9. Effects of exotic species on Yellowstone's grizzly bears

    Science.gov (United States)

    Reinhart, Daniel P.; Haroldson, Mark A.; Mattson, D.J.; Gunther, Kerry A.

    2001-01-01

    Humans have affected grizzly bears (Ursus arctos horribilis) by direct mortality, competition for space and resources, and introduction of exotic species. Exotic organisms that have affected grizzly bears in the Greater Yellowstone Area include common dandelion (Taraxacum officinale), nonnative clovers (Trifolium spp.), domesticated livestock, bovine brucellosis (Brucella abortus), lake trout (Salvelinus namaycush), and white pine blister rust (Cronartium ribicola). Some bears consume substantial amounts of dandelion and clover. However, these exotic foods provide little digested energy compared to higher-quality bear foods. Domestic livestock are of greater energetic value, but use of this food by bears often leads to conflicts with humans and subsequent increases in bear mortality. Lake trout, blister rust, and brucellosis diminish grizzly bears foods. Lake trout prey on native cutthroat trout (Oncorhynchus clarkii) in Yellowstone Lake; white pine blister rust has the potential to destroy native whitebark pine (Pinus albicaulis) stands; and management response to bovine brucellosis, a disease found in the Yellowstone bison (Bison bison) and elk (Cervus elaphus), could reduce populations of these 2 species. Exotic species will likely cause more harm than good for Yellowstone grizzly bears. Managers have few options to mitigate or contain the impacts of exotics on Yellowstone's grizzly bears. Moreover, their potential negative impacts have only begun to unfold. Exotic species may lead to the loss of substantial highquality grizzly bear foods, including much of the bison, trout, and pine seeds that Yellowstone grizzly bears currently depend upon.

  10. Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout

    Science.gov (United States)

    Ottinger, Christopher A.; Honeyfield, Dale C.; Densmore, Christine L.; Iwanowicz, Luke R.

    2012-01-01

    Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to the T-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNP-KLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentrations were evaluated for possible differences in total anti-TNP activity as well as differences in response kinetics. Associations between anti-TNP activity and muscle and liver thiamine concentrations as well as ratios of muscle-to-liver thiamine to anti-TNP activity were also examined. Thiamine-depleted lake trout that were injected with TNP-LPS exhibited significantly more anti-TNP activity than thiamine-replete fish. The depleted fish injected with TNP-LPS also exhibited significantly different response kinetics relative to thiamine-replete lake trout. No differences in activity or kinetics were observed between the thiamine-replete and -depleted fish injected with TNP-KLH or in the DPBS negative controls. Anti-TNP activity in thiamine-depleted lake trout injected with TNP-KLH was positively associated with muscle thiamine pyrophosphate (thiamine diphosphate; TPP) concentration. A negative association was observed between the ratio of muscle-to-liver TPP and T-independent responses. No significant associations between anti-TNP activity and tissue thiamine concentration were observed in the thiamine-replete fish. We demonstrated that thiamine deficiency leads to alterations in both T-dependent and T-independent immune responses in lake trout.

  11. Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout.

    Science.gov (United States)

    Ottinger, Christopher A; Honeyfield, Dale C; Densmore, Christine L; Iwanowicz, Luke R

    2012-12-01

    Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to the T-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNP-KLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentrations were evaluated for possible differences in total anti-TNP activity as well as differences in response kinetics. Associations between anti-TNP activity and muscle and liver thiamine concentrations as well as ratios of muscle-to-liver thiamine to anti-TNP activity were also examined. Thiamine-depleted lake trout that were injected with TNP-LPS exhibited significantly more anti-TNP activity than thiamine-replete fish. The depleted fish injected with TNP-LPS also exhibited significantly different response kinetics relative to thiamine-replete lake trout. No differences in activity or kinetics were observed between the thiamine-replete and -depleted fish injected with TNP-KLH or in the DPBS negative controls. Anti-TNP activity in thiamine-depleted lake trout injected with TNP-KLH was positively associated with muscle thiamine pyrophosphate (thiamine diphosphate; TPP) concentration. A negative association was observed between the ratio of muscle-to-liver TPP and T-independent responses. No significant associations between anti-TNP activity and tissue thiamine concentration were observed in the thiamine-replete fish. We demonstrated that thiamine deficiency leads to alterations in both T-dependent and T-independent immune responses in lake trout.

  12. Effect of rearing density on poststocking survival of lake trout in Lake Ontario

    Science.gov (United States)

    Elrod, Joseph H.; Ostergaard, David E.; Schneider, Clifford P.

    1989-01-01

    Six paired lots of yearling lake trout (Salvelinus namaycush) reared at densities of 41,000 and 51,000 fish per raceway during their last 9 months in the hatchery were stocked in Lake Ontario. Poststocking survival of the high-density (HD) and low-density (LD) fish was not different for the 1982 year-class. However, for the 1983 year-class, mean survival was significantly different between HD and LD fish (P Mean survival of HD fish was only 76% that of LD fish (P Mean size at stocking was not different for HD and LD fish of the 1982 year-class, but for the 1983 year-class, the LD fish were 6% longer and 22% heavier than the HD fish. Mean lengths and weights of LD and HD fish were not different in samples collected in Lake Ontario at age 2 and older. Size at stocking was not likely the factor that caused the difference in survival. Rather, the rearing conditions (probably water exchange rate in relation to number of fish in the raceway) that resulted in slower growth of the HD fish of the 1983 year-class also caused them to be poorer physiologically than the LD fish. The number of yearling lake trout per rearing unit that will result in maximum contribution to populations in the Great Lakes after stocking may be lower than the rearing densities customarily used at some hatcheries.

  13. Geographic Place Names, GNIS data for Atkinson, Bacon, Ben Hill, Berrien, Brantley, Brooks, Charlton, Clinch, Coffee, Cook, Echols, Irwin, Lanier, Lowndes, Pierce, Tift, Turner, Ware counties., Published in 1999, 1:7200 (1in=600ft) scale, Southern Georgia Regional Commission.

    Data.gov (United States)

    NSGIC Regional | GIS Inventory — Geographic Place Names dataset current as of 1999. GNIS data for Atkinson, Bacon, Ben Hill, Berrien, Brantley, Brooks, Charlton, Clinch, Coffee, Cook, Echols, Irwin,...

  14. Physical and profile oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-09-02 to 2010-09-06 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0084590)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical and profile oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-09-02 to 2010-09-06 in response to the Deepwater...

  15. Physical and profile oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-29 to 2010-09-02 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0084589)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical and profile oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-29 to 2010-09-02 in response to the Deepwater...

  16. Chemical, physical, profile and other oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-18 to 2010-05-22 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069044)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile, laboratory analysis, tows and underway oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-18...

  17. Adaptive Management of Bull Trout Populations in the Lemhi Basin

    Science.gov (United States)

    Peterson, James T.; Tyre, Andrew J.; Converse, Sarah J.; Bogich, Tiffany L.; Miller, Damien; Post van der Burg, Max; Thomas, Carmen; Thompson, Ralph J.; Wood, Jeri; Brewer, Donna; Runge, Michael C.

    2011-01-01

    The bull trout Salvelinus confluentus, a stream-living salmonid distributed in drainages of the northwestern United States, is listed as threatened under the Endangered Species Act because of rangewide declines. One proposed recovery action is the reconnection of tributaries in the Lemhi Basin. Past water use policies in this core area disconnected headwater spawning sites from downstream habitat and have led to the loss of migratory life history forms. We developed an adaptive management framework to analyze which types of streams should be prioritized for reconnection under a proposed Habitat Conservation Plan. We developed a Stochastic Dynamic Program that identified optimal policies over time under four different assumptions about the nature of the migratory behavior and the effects of brook trout Salvelinus fontinalis on subpopulations of bull trout. In general, given the current state of the system and the uncertainties about the dynamics, the optimal policy would be to connect streams that are currently occupied by bull trout. We also estimated the value of information as the difference between absolute certainty about which of our four assumptions were correct, and a model averaged optimization assuming no knowledge. Overall there is little to be gained by learning about the dynamics of the system in its current state, although in other parts of the state space reducing uncertainties about the system would be very valuable. We also conducted a sensitivity analysis; the optimal decision at the current state does not change even when parameter values are changed up to 75% of the baseline values. Overall, the exercise demonstrates that it is possible to apply adaptive management principles to threatened and endangered species, but logistical and data availability constraints make detailed analyses difficult.

  18. 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (Salvelinus alpinus

    Directory of Open Access Journals (Sweden)

    Modig Carina

    2004-09-01

    Full Text Available Abstract This study was performed to investigate stress effects on the synthesis of egg yolk precursor, vitellogenin (Vtg in Arctic char (Salvelinus alpinus. In particular the effect of cortisol (F was determined since this stress hormone has been suggested to interfere with vitellogenesis and is upregulated during sexual maturation in teleosts. Arctic char Vtg was purified and polyclonal antibodies were produced in order to develop tools to study regulation of vitellogenesis. The Vtg antibodies were used to develop an enzyme-linked immunosorbent assay. The corresponding Vtg cDNA was cloned from a hepatic cDNA library in order to obtain DNA probes to measure Vtg mRNA expression. Analysis of plasma from juvenile Arctic char, of both sexes, exposed to different steroids showed that production of Vtg was induced in a dose dependent fashion by 17β-estradiol (E2, estrone and estriol. Apart from estrogens a high dose of F also upregulated Vtg. In addition, F, progesterone (P and tamoxifen were tested to determine these compounds ability to modulate E2 induced Vtg synthesis at both the mRNA and protein level. Tamoxifen was found to inhibit E2 induced Vtg mRNA and protein upregulation. P did not alter the Vtg induction while F reduced the Vtg protein levels without affecting the Vtg mRNA levels. Furthermore the inhibition of Vtg protein was found to be dose dependent. Thus, the inhibitory effect of F on Vtg appears to be mediated at the post-transcriptional level.

  19. Observations of Inland Snowpack-driven Bromine Chemistry near the Brooks Range, Alaska

    Science.gov (United States)

    Peterson, P.; Pöhler, D.; Sihler, H.; Zielcke, J.; S., General; Friess, U.; Platt, U.; Simpson, W. R.; Nghiem, S. V.; Shepson, P. B.; Stirm, B. H.; Pratt, K.

    2017-12-01

    The snowpack produces high amounts of reactive bromine in the polar regions during spring. The resulting atmospheric bromine chemistry depletes boundary layer ozone to near-zero levels and alters oxidation of atmospheric pollutants, particularly elemental mercury. To improve our understanding of the spatial extent of this bromine chemistry in Arctic coastal regions, the Purdue Airborne Laboratory for Atmospheric Research (ALAR), equipped with the Heidelberg Imaging differential optical absorption spectroscopy (DOAS) instrument, measured the spatial distribution of BrO, an indicator of active bromine chemistry, over northern Alaska during the March 2012 BRomine Ozone Mercury Experiment (BROMEX). Here we show that this bromine chemistry, commonly associated with snow-covered sea ice regions in the Arctic Ocean, is active 200 km inland in the foothills of the Brooks Range. Profiles retrieved from limb-viewing measurements show this event was located near the snowpack surface, with measured BrO mole ratios of 20 pmol mol-1 in a 500 m thick layer. This observed bromine chemistry is likely enabled by deposition of transported sea salt aerosol or gas phase bromine species from prior activation events to the snowpack. These observations of halogen activation hundreds of km from the coast suggest the impacts of this springtime bromine chemistry are not restricted to sea ice regions and directly adjacent coastal regions.

  20. Morphological and molecular confirmation of Myxobolus cerebralis myxospores infecting wild‑caught and cultured trout in North Carolina (SE USA).

    Science.gov (United States)

    Ruiz, Carlos F; Rash, Jacob M; Arias, Cova R; Besler, Doug A; Orélis-Ribeiro, Raphael; Womble, Matthew R; Roberts, Jackson R; Warren, Micah B; Ray, Candis L; Lafrentz, Stacey; Bullard, Stephen A

    2017-11-21

    We used microscopy and molecular biology to provide the first documentation of infections of Myxobolus cerebralis (Myxozoa: Myxobolidae), the etiological agent of whirling disease, in trout (Salmonidae) from North Carolina (USA) river basins. A total of 1085 rainbow trout Oncorhynchus mykiss, 696 brown trout Salmo trutta, and 319 brook trout Salvelinus fontinalis from 43 localities across 9 river basins were screened. Myxospores were observed microscopically in pepsin-trypsin digested heads of rainbow and brown trout from the Watauga River Basin. Those infections were confirmed using the prescribed nested polymerase chain reaction (PCR; 18S rDNA), which also detected infections in rainbow, brown, and brook trout from the French Broad River Basin and the Yadkin Pee-Dee River Basin. Myxospores were 9.0-10.0 µm (mean ± SD = 9.6 ± 0.4; N = 119) long, 8.0-10.0 µm (8.8 ± 0.6; 104) wide, and 6.0-7.5 µm (6.9 ± 0.5; 15) thick and had polar capsules 4.0-6.0 µm (5.0 ± 0.5; 104) long, 2.5-3.5 µm (3.1 ± 0.3; 104) wide, and with 5 or 6 polar filament coils. Myxospores from these hosts and rivers were morphologically indistinguishable and molecularly identical, indicating conspecificity, and the resulting 18S rDNA and ITS-1 sequences derived from these myxospores were 99.5-100% and 99.3-99.8% similar, respectively, to published GenBank sequences ascribed to M. cerebralis. This report comprises the first taxonomic circumscription and molecular confirmation of M. cerebralis in the southeastern USA south of Virginia.

  1. Nesting by Golden Eagles on the North Slope of the Brooks Range in Northeastern Alaska

    Science.gov (United States)

    Young, Donald D.; McIntyre, Carol L.; Bente, Peter J.; McCabe, Thomas R.; Ambrose, Robert E.

    1995-01-01

    Twenty-two Golden Eagle (Aquila chrysaetos) nesting territories and 31 occupied eagle nests were documented on the north slope of the Brooks Range in northeastern Alaska, 1988-1990, in an area previously thought to be marginal breeding habitat for eagles. The mean number of young/successful nest was 1.25 in 1988, 1.27 in 1989, and 1.13 in 1990; means did not differ significantly among years. Eighty percent (20/25) of the nestlings for which age was estimated were assumed to have successfully fledged. Nesting success was 79% (11/14) in 1989, the only year nesting success could be determined. Laying dates ranged from 23 March (1990) to 11 May (1989) with mean estimated laying dates differing significantly among years. Annual variation in nesting phenology coincided with annual differences in snow accumulations during spring. These results indicate that Golden Eagles consistently and successfully breed at the northern extent of their range in Alaska, although, productivity may be lower than that for eagles at more southern latitudes.

  2. Theoretical Insight Into the Empirical Tortuosity-Connectivity Factor in the Burdine-Brooks-Corey Water Relative Permeability Model

    Science.gov (United States)

    Ghanbarian, Behzad; Ioannidis, Marios A.; Hunt, Allen G.

    2017-12-01

    A model commonly applied to the estimation of water relative permeability krw in porous media is the Burdine-Brooks-Corey model, which relies on a simplified picture of pores as a bundle of noninterconnected capillary tubes. In this model, the empirical tortuosity-connectivity factor is assumed to be a power law function of effective saturation with an exponent (μ) commonly set equal to 2 in the literature. Invoking critical path analysis and using percolation theory, we relate the tortuosity-connectivity exponent μ to the critical scaling exponent t of percolation that characterizes the power law behavior of the saturation-dependent electrical conductivity of porous media. We also discuss the cause of the nonuniversality of μ in terms of the nonuniversality of t and compare model estimations with water relative permeability from experiments. The comparison supports determining μ from the electrical conductivity scaling exponent t, but also highlights limitations of the model.

  3. The Jeffers Brook diorite-granodiorite pluton: style of emplacement and role of volatiles at various crustal levels in Avalonian appinites, Canadian Appalachians

    Science.gov (United States)

    Pe-Piper, Georgia; Piper, David J. W.

    2018-04-01

    Small appinite plutons ca. 610 Ma outcrop in the peri-Gondwanan Avalon terrane of northern Nova Scotia, with different structural levels exposed. Field mapping shows that the Jeffers Brook pluton is a laccolith emplaced along an upper crustal thrust zone, likely in a dilational jog in a regional dextral strike-slip system. The oldest rocks are probably mafic sills, which heated the area facilitating emplacement of intermediate magmas. Cross-cutting relationships show that both mafic and intermediate magmas were supplied throughout the history of pluton emplacement. The modal composition, mineral chemistry, and bulk chemistry of gabbro, diorite, tonalite, granodiorite, and granite have been studied in the main plutonic phases, dykes, and sills, and mafic microgranular enclaves. As with the type appinites in the Scottish Caledonides, the pluton shows evidence of high water content: the dominance of hornblende, locally within pegmatitic texture; vesicles and irregular felsic patches in enclaves; and late aplite dykes. Analyzed mafic microgranular enclaves are geochemically similar to larger diorite bodies in the pluton. Tonalite-granodiorite is distinct from the diorite in trace-element geochemistry and radiogenic isotopes. Elsewhere to the east, similar rocks of the same age form vertically sheeted complexes in major shear zones; hornblende chemistry shows that they were emplaced at a deeper upper crustal level. This implies that little of the observed geochemical variability in the Jeffers Brook pluton was developed within the pluton. The general requirements to form appinites are proposed to be small magma volumes of subduction-related magmas that reach the upper crust because of continual heating by mafic magmas moving through strike-slip fault pathways and trapping of aqueous fluids rather than venting through volcanic activity.

  4. Chemical, physical, profile and other oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-07 to 2010-05-12 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0084555)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile, tows and underway oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-07 to 2010-05-12 in...

  5. Integrative phylogeography of Calotriton newts (Amphibia, Salamandridae, with special remarks on the conservation of the endangered Montseny brook newt (Calotriton arnoldi.

    Directory of Open Access Journals (Sweden)

    Emilio Valbuena-Ureña

    Full Text Available The genus Calotriton includes two species of newts highly adapted to live in cold and fast-flowing mountain springs. The Pyrenean brook newt (Calotriton asper, restricted to the Pyrenean region, and the Montseny brook newt (Calotriton arnoldi, endemic to the Montseny massif and one of the most endangered amphibian species in Europe. In the present manuscript, we use an integrative approach including species distribution modeling (SDM, molecular analyses of mitochondrial and nuclear DNA sequence data and morphology to unravel the historical processes that have contributed to shaping the biogeography and genetic structure of the genus Calotriton, with special emphasis on the conservation of C. arnoldi. The results of the molecular analyses confirm that, despite having originated recently, being ecologically similar and geographically very close, there is no signal of hybridization between C. asper and C. arnoldi. SDM results suggest that tough environmental conditions on mountains tops during glacial periods, together with subsequent warmer periods could have prevented the contact between the two species. Within the critically endangered C. arnoldi, a high genetic structure is revealed despite its extremely small distribution range compared to C. asper. Haplotype networks, AMOVA and SAMOVA analyses suggest that two distinct groups of populations can be clearly differentiated with absence of gene flow. This is in concordance with morphological differentiation and correlates with its geographical distribution, as the two groups are situated on the eastern and western sides of a river valley that acts as a barrier. The genetic and morphological results are highly important for the ongoing conservation program of C. arnoldi and strongly justify the management of this species into at least two independent evolutionary significant units (eastern and western sectors to guarantee the long-term population viability.

  6. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-04 to 2010-07-08 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069051)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-04 to 2010-07-08 in...

  7. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-30 to 2010-06-02 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069046)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-30 to 2010-06-02 in...

  8. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-03 to 2010-08-07 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069055)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-03 to 2010-08-07 in...

  9. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-21 to 2010-08-25 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069090)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-21 to 2010-08-25 in...

  10. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-16 to 2010-07-20 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069053)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-16 to 2010-07-20 in...

  11. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-22 to 2010-06-26 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069050)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-22 to 2010-06-26 in...

  12. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-15 to 2010-08-19 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069057)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-15 to 2010-08-19 in...

  13. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-09 to 2010-08-12 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069056)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-08-09 to 2010-08-12 in...

  14. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-28 to 2010-08-01 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069054)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-28 to 2010-08-01 in...

  15. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-23 to 2010-05-25 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069045)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-23 to 2010-05-25 in...

  16. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-10 to 2010-06-14 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069048)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-10 to 2010-06-14 in...

  17. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-09-07 to 2010-09-11 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0074853)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-09-07 to 2010-09-11 in...

  18. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-16 to 2010-06-20 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069049)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-16 to 2010-06-20 in...

  19. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-14 to 2010-05-18 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0074372)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-05-14 to 2010-05-18 in...

  20. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-10 to 2010-07-14 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069052)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-07-10 to 2010-07-14 in...

  1. Chemical, physical, profile and laboratory analysis oceanographic data collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-04 to 2010-06-08 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0069047)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Chemical, physical, profile and laboratory analysis oceanographic data were collected aboard the Brooks McCall in the Gulf of Mexico from 2010-06-04 to 2010-06-08 in...

  2. Results of the radiological survey of the Saddle River's East Bank, from State Highway 46 to the River's confluence with Lodi Brook, Lodi, New Jersey (LJ083)

    International Nuclear Information System (INIS)

    Foley, R.D.; Floyd, L.M.

    1989-11-01

    Maywood Chemical Works (MCW) of Maywood, New Jersey, generated process wastes and residues associated with the production and refining of thorium and thorium compounds from monazite ores from 1916 to 1956. MCW supplied rare earth metals and thorium compounds to the Atomic Energy Commission and various other government agencies from the late 1940s to the mid-1950s. Area residents used the sandlike waste from this thorium extraction process mixed with tea and cocoa leaves as mulch in their yards. Some of these contaminated wastes were also eroded from the site into Lodi Brook. At the request of the US Department of Energy (DOE), a group from Oak Ridge National Laboratory conducts investigative radiological surveys of properties in the vicinity of MCW to determine whether a property is contaminated with radioactive residues, principally 232 Th, derived from the MCW site. The survey typically includes direct measurement of gamma radiation levels and soil sampling for radionuclide analyses. The survey of this site, the Saddle River's East Bank, from State Highway 46 to the River's Confluence with Lodi Brook, Lodi, New Jersey (LJ083), was conducted during 1988. Results of the survey demonstrated no radionuclide concentrations in excess of the DOE Formerly Utilized Sites Remedial Action Program criteria. The radionuclide distributions were not significantly different from normal background levels in the northern New Jersey area. 4 refs., 2 figs., 3 tabs

  3. Bull Trout Population Assessment in the Columbia River Gorge : Annual Report 2000.

    Energy Technology Data Exchange (ETDEWEB)

    Byrne, Jim; McPeak, Ron

    2001-02-01

    We summarized existing knowledge regarding the known distribution of bull trout (Salvelinus confluentus) across four sub-basins in the Columbia River Gorge in Washington. The Wind River, Little White Salmon River, White Salmon River, and the Klickitat River sub-basins were analyzed. Cold water is essential to the survival, spawning, and rearing of bull trout. We analyzed existing temperature data, installed Onset temperature loggers in the areas of the four sub-basins where data was not available, and determined that mean daily water temperatures were <15 C and appropriate for spawning and rearing of bull trout. We snorkel surveyed more than 74 km (46.25 mi.) of rivers and streams in the four sub-basins (13.8 km at night and 60.2 km during the day) and found that night snorkeling was superior to day snorkeling for locating bull trout. Surveys incorporated the Draft Interim Protocol for Determining Bull Trout Presence (Peterson et al. In Press). However, due to access and safety issues, we were unable to randomly select sample sites nor use block nets as recommended. Additionally, we also implemented the Bull Trout/Dolly Varden sampling methodology described in Bonar et al. (1997). No bull trout were found in the Wind River, Little White Salmon, or White Salmon River sub-basins. We found bull trout in the West Fork Klickitat drainage of the Klickitat River Sub-basin. Bull trout averaged 6.7 fish/100m{sup 2} in Trappers Creek, 2.6 fish/100m{sup 2} on Clearwater Creek, and 0.4 fish/100m{sup 2} in Little Muddy Creek. Bull trout was the only species of salmonid encountered in Trappers Creek and dominated in Clearwater Creek. Little Muddy Creek was the only creek where bull trout and introduced brook trout occurred together. We found bull trout only at night and typically in low flow regimes. A single fish, believed to be a bull trout x brook trout hybrid, was observed in the Little Muddy Creek. Additional surveys are needed in the West Fork Klickitat and mainstem

  4. Bull trout population assessment in the Columbia River Gorge/annual report fy2000; ANNUAL

    International Nuclear Information System (INIS)

    Byrne, Jim; McPeak, Ron

    2001-01-01

    We summarized existing knowledge regarding the known distribution of bull trout (Salvelinus confluentus) across four sub-basins in the Columbia River Gorge in Washington. The Wind River, Little White Salmon River, White Salmon River, and the Klickitat River sub-basins were analyzed. Cold water is essential to the survival, spawning, and rearing of bull trout. We analyzed existing temperature data, installed Onset temperature loggers in the areas of the four sub-basins where data was not available, and determined that mean daily water temperatures were and lt;15 C and appropriate for spawning and rearing of bull trout. We snorkel surveyed more than 74 km (46.25 mi.) of rivers and streams in the four sub-basins (13.8 km at night and 60.2 km during the day) and found that night snorkeling was superior to day snorkeling for locating bull trout. Surveys incorporated the Draft Interim Protocol for Determining Bull Trout Presence (Peterson et al. In Press). However, due to access and safety issues, we were unable to randomly select sample sites nor use block nets as recommended. Additionally, we also implemented the Bull Trout/Dolly Varden sampling methodology described in Bonar et al. (1997). No bull trout were found in the Wind River, Little White Salmon, or White Salmon River sub-basins. We found bull trout in the West Fork Klickitat drainage of the Klickitat River Sub-basin. Bull trout averaged 6.7 fish/100m(sup 2) in Trappers Creek, 2.6 fish/100m(sup 2) on Clearwater Creek, and 0.4 fish/100m(sup 2) in Little Muddy Creek. Bull trout was the only species of salmonid encountered in Trappers Creek and dominated in Clearwater Creek. Little Muddy Creek was the only creek where bull trout and introduced brook trout occurred together. We found bull trout only at night and typically in low flow regimes. A single fish, believed to be a bull trout x brook trout hybrid, was observed in the Little Muddy Creek. Additional surveys are needed in the West Fork Klickitat and mainstem

  5. The effect of rapid and sustained decompression on barotrauma in juvenile brook lamprey and Pacific lamprey: implications for passage at hydroelectric facilities

    Energy Technology Data Exchange (ETDEWEB)

    Colotelo, Alison HA; Pflugrath, Brett D.; Brown, Richard S.; Brauner, Colin J.; Mueller, Robert P.; Carlson, Thomas J.; Deng, Zhiqun; Ahmann, Martin L.; Trumbo, Bradly A.

    2012-10-01

    Fish passing downstream through hydroelectric facilities may pass through hydroturbines where they experience a rapid decrease in barometric pressure as they pass by turbine blades, which can lead to barotraumas including swim bladder rupture, exopthalmia, emboli, and hemorrhaging. In juvenile Chinook salmon, the main mechanism for injury is thought to be expansion of existing gases (particularly those present in the swim bladder) and the rupture of the swim bladder ultimately leading to exopthalmia, emboli and hemorrhaging. In fish that lack a swim bladder, such as lamprey, the rate and severity of barotraumas due to rapid decompression may be reduced however; this has yet to be extensively studied. Another mechanism for barotrauma can be gases coming out of solution and the rate of this occurrence may vary among species. In this study, juvenile brook and Pacific lamprey acclimated to 146.2 kPa (equivalent to a depth of 4.6 m) were subjected to rapid (<1 sec; brook lamprey only) or sustained decompression (17 minutes) to a very low pressure (13.8 kPa) using a protocol previously applied to juvenile Chinook salmon. No mortality or evidence of barotraumas, as indicated by the presence of hemorrhages, emboli or exopthalmia, were observed during rapid or sustained decompression, nor following recovery for up to 120 h following sustained decompression. In contrast, mortality or injury would be expected for 97.5% of juvenile Chinook salmon exposed to a similar rapid decompression to these very low pressures. Additionally, juvenile Chinook salmon experiencing sustained decompression died within 7 minutes, accompanied by emboli in the fins and gills and hemorrhaging in the tissues. Thus, juvenile lamprey may not be susceptible to barotraumas associated with hydroturbine passage to the same degree as juvenile salmonids, and management of these species should be tailored to their specific morphological and physiological characteristics.

  6. Network Analysis Highlights Complex Interactions between Pathogen, Host and Commensal Microbiota

    Science.gov (United States)

    Boutin, Sébastien; Bernatchez, Louis; Audet, Céline; Derôme, Nicolas

    2013-01-01

    Interactions between bacteria and their host represent a full continuum from pathogenicity to mutualism. From an evolutionary perspective, host-bacteria relationships are no longer considered a two-component system but rather a complex network. In this study, we focused on the relationship between brook charr (Salvelinus fontinalis) and bacterial communities developing on skin mucus. We hypothesized that stressful conditions such as those occurring in aquaculture production induce shifts in the bacterial community of healthy fish, thus allowing pathogens to cause infections. The results showed that fish skin mucus microbiota taxonomical structure is highly specific, its diversity being partly influenced by the surrounding water bacterial community. Two types of taxonomic co-variation patterns emerged across 121 contrasted communities’ samples: one encompassing four genera well known for their probiotic properties, the other harboring five genera mostly associated with pathogen species. The homeostasis of fish bacterial community was extensively disturbed by induction of physiological stress in that both: 1) the abundance of probiotic-like bacteria decreased after stress exposure; and 2) pathogenic bacteria increased following stress exposure. This study provides further insights regarding the role of mutualistic bacteria as a primary host protection barrier. PMID:24376845

  7. Network analysis highlights complex interactions between pathogen, host and commensal microbiota.

    Directory of Open Access Journals (Sweden)

    Sébastien Boutin

    Full Text Available Interactions between bacteria and their host represent a full continuum from pathogenicity to mutualism. From an evolutionary perspective, host-bacteria relationships are no longer considered a two-component system but rather a complex network. In this study, we focused on the relationship between brook charr (Salvelinus fontinalis and bacterial communities developing on skin mucus. We hypothesized that stressful conditions such as those occurring in aquaculture production induce shifts in the bacterial community of healthy fish, thus allowing pathogens to cause infections. The results showed that fish skin mucus microbiota taxonomical structure is highly specific, its diversity being partly influenced by the surrounding water bacterial community. Two types of taxonomic co-variation patterns emerged across 121 contrasted communities' samples: one encompassing four genera well known for their probiotic properties, the other harboring five genera mostly associated with pathogen species. The homeostasis of fish bacterial community was extensively disturbed by induction of physiological stress in that both: 1 the abundance of probiotic-like bacteria decreased after stress exposure; and 2 pathogenic bacteria increased following stress exposure. This study provides further insights regarding the role of mutualistic bacteria as a primary host protection barrier.

  8. Mercury levels in herring gulls and fish: 42 years of spatio-temporal trends in the Great Lakes.

    Science.gov (United States)

    Blukacz-Richards, E Agnes; Visha, Ariola; Graham, Matthew L; McGoldrick, Daryl L; de Solla, Shane R; Moore, David J; Arhonditsis, George B

    2017-04-01

    Total mercury levels in aquatic birds and fish communities have been monitored across the Canadian Great Lakes by Environment and Climate Change Canada (ECCC) for the past 42 years (1974-2015). These data (22 sites) were used to examine spatio-temporal variability of mercury levels in herring gull (Larus argentatus) eggs, lake trout (Salvelinus namaycush), walleye (Sander vitreus), and rainbow smelt (Osmerus mordax). Trends were quantified with dynamic linear models, which provided time-variant rates of change of mercury concentrations. Lipid content (in both fish and eggs) and length in fish were used as covariates in all models. For the first three decades, mercury levels in gull eggs and fish declined at all stations. In the 2000s, trends for herring gull eggs reversed at two sites in Lake Erie and two sites in Lake Ontario. Similar trend reversals in the 2000s were observed for lake trout in Lake Superior and at a single station in Lake Ontario. Mercury levels in lake trout continued to slowly decline at all of the remaining stations, except for Lake Huron, where the levels remained stable. A post-hoc Bayesian regression analysis suggests strong trophic interactions between herring gulls and rainbow smelt in Lake Superior and Lake Ontario, but also pinpoints the likelihood of a trophic decoupling in Lake Huron and Lake Erie. Continued monitoring of mercury levels in herring gulls and fish is required to consolidate these trophic shifts and further evaluate their broader implications. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  9. Evaluating stocking efficacy in an ecosystem undergoing oligotrophication

    Science.gov (United States)

    Kao, Yu-Chun; Rogers, Mark W.; Bunnell, David B.

    2017-01-01

    Oligotrophication has negatively affected fisheries production in many freshwater ecosystems and could conceivably reduce the efficacy of stockings used to enhance fisheries. In Lake Michigan, offshore oligotrophication has occurred since the 1970s, owing to reductions in total phosphorus (TP) inputs and nearshore sequestration of TP by nonindigenous dreissenid mussels. We evaluated simultaneous effects of stock enhancement and oligotrophication on salmonine species (Chinook salmon Oncorhynchus tshawytscha, lake trout Salvelinus namaycush, and steelhead O. mykiss) that support valuable recreational fisheries. We employed a novel application of an Ecopath with Ecosim model by conducting a full factorial simulation experiment. Our design included multiple levels of salmonine stocking, consumption by invasive quagga mussels (Dreissena bugensis), and TP that were informed by manager interests. Under all levels of TP and quagga mussel consumption, our results showed that stock enhancement could still increase salmonine biomass, but positive responses were stronger for lake trout and steelhead than Chinook salmon. Simulations showed that quagga mussel consumption has deleterious effects on pelagic-oriented prey fishes and Chinook salmon, which feed almost exclusively on the pelagic-oriented alewife (Alosa pseudoharengus). In summary, results from our simulation experiment suggested that lake trout and steelhead are better suited to the current ecosystem than Chinook salmon, and therefore, stock enhancement provides the highest gains for these two species. Furthermore, simulated biomass of all recreational salmonine species increased with increasing TP, indicating the need for managers to consider how potential future oligotrophication will limit the carrying capacity of salmonine biomass in Lake Michigan

  10. Coupling age-structured stock assessment and fish bioenergetics models: a system of time-varying models for quantifying piscivory patterns during the rapid trophic shift in the main basin of Lake Huron

    Science.gov (United States)

    He, Ji X.; Bence, James R.; Madenjian, Charles P.; Pothoven, Steven A.; Dobiesz, Norine E.; Fielder, David G.; Johnson, James E.; Ebener, Mark P.; Cottrill, Adam R.; Mohr, Lloyd C.; Koproski, Scott R.

    2015-01-01

    We quantified piscivory patterns in the main basin of Lake Huron during 1984–2010 and found that the biomass transfer from prey fish to piscivores remained consistently high despite the rapid major trophic shift in the food webs. We coupled age-structured stock assessment models and fish bioenergetics models for lake trout (Salvelinus namaycush), Chinook salmon (Oncorhynchus tshawytscha), walleye (Sander vitreus), and lake whitefish (Coregonus clupeaformis). The model system also included time-varying parameters or variables of growth, length–mass relations, maturity schedules, energy density, and diets. These time-varying models reflected the dynamic connections that a fish cohort responded to year-to-year ecosystem changes at different ages and body sizes. We found that the ratio of annual predation by lake trout, Chinook salmon, and walleye combined with the biomass indices of age-1 and older alewives (Alosa pseudoharengus) and rainbow smelt (Osmerus mordax) increased more than tenfold during 1987–2010, and such increases in predation pressure were structured by relatively stable biomass of the three piscivores and stepwise declines in the biomass of alewives and rainbow smelt. The piscivore stability was supported by the use of alternative energy pathways and changes in relative composition of the three piscivores. In addition, lake whitefish became a new piscivore by feeding on round goby (Neogobius melanostomus). Their total fish consumption rivaled that of the other piscivores combined, although fish were still a modest proportion of their diet. Overall, the use of alternative energy pathways by piscivores allowed the increases in predation pressure on dominant diet species.

  11. Lake trout in northern Lake Huron spawn on submerged drumlins

    Science.gov (United States)

    Riley, Stephen C.; Binder, Thomas; Wattrus, Nigel J.; Faust, Matthew D.; Janssen, John; Menzies, John; Marsden, J. Ellen; Ebener, Mark P.; Bronte, Charles R.; He, Ji X.; Tucker, Taaja R.; Hansen, Michael J.; Thompson, Henry T.; Muir, Andrew M.; Krueger, Charles C.

    2014-01-01

    Recent observations of spawning lake trout Salvelinus namaycush near Drummond Island in northern Lake Huron indicate that lake trout use drumlins, landforms created in subglacial environments by the action of ice sheets, as a primary spawning habitat. From these observations, we generated a hypothesis that may in part explain locations chosen by lake trout for spawning. Most salmonines spawn in streams where they rely on streamflows to sort and clean sediments to create good spawning habitat. Flows sufficient to sort larger sediment sizes are generally lacking in lakes, but some glacial bedforms contain large pockets of sorted sediments that can provide the interstitial spaces necessary for lake trout egg incubation, particularly if these bedforms are situated such that lake currents can penetrate these sediments. We hypothesize that sediment inclusions from glacial scavenging and sediment sorting that occurred during the creation of bedforms such as drumlins, end moraines, and eskers create suitable conditions for lake trout egg incubation, particularly where these bedforms interact with lake currents to remove fine sediments. Further, these bedforms may provide high-quality lake trout spawning habitat at many locations in the Great Lakes and may be especially important along the southern edge of the range of the species. A better understanding of the role of glacially-derived bedforms in the creation of lake trout spawning habitat may help develop powerful predictors of lake trout spawning locations, provide insight into the evolution of unique spawning behaviors by lake trout, and aid in lake trout restoration in the Great Lakes.

  12. Quantification of 15 bile acids in lake charr feces by ultra-high performance liquid chromatography–tandem mass spectrometry

    Science.gov (United States)

    Li, Ke; Buchinger, Tyler J.; Bussy, Ugo; Fissette, Skye D.; Johnson, Nicholas; Li, Weiming

    2015-01-01

    Many fishes are hypothesized to use bile acids (BAs) as chemical cues, yet quantification of BAs in biological samples and the required methods remain limited. Here, we present an UHPLC–MS/MS method for simultaneous, sensitive, and rapid quantification of 15 BAs, including free, taurine, and glycine conjugated BAs, and application of the method to fecal samples from lake charr (Salvelinus namaycush). The analytes were separated on a C18 column with acetonitrile–water (containing 7.5 mM ammonium acetate and 0.1% formic acid) as mobile phase at a flow rate of 0.25 mL/min for 12 min. BAs were monitored with a negative electrospray triple quadrupole mass spectrometer (Xevo TQ-S™). Calibration curves of 15 BAs were linear over the concentration range of 1.00–5,000 ng/mL. Validation revealed that the method was specific, accurate, and precise. The method was applied to quantitative analysis of feces extract of fry lake charr and the food they were eating. The concentrations of analytes CA, TCDCA, TCA, and CDCA were 242.3, 81.2, 60.7, and 36.2 ng/mg, respectively. However, other taurine conjugated BAs, TUDCA, TDCA, and THDCA, were not detected in feces of lake charr. Interestingly, TCA and TCDCA were detected at high concentrations in food pellets, at 71.9 and 38.2 ng/mg, respectively. Application of the method to feces samples from lake charr supported a role of BAs as chemical cues, and will enhance further investigation of BAs as chemical cues in other fish species.

  13. Factors influencing the distribution of native bull trout and westslope cutthroat trout in western Glacier National Park, Montana

    Science.gov (United States)

    D'Angelo, Vincent S.; Muhlfeld, Clint C.

    2013-01-01

    The widespread declines of native bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) populations prompted researchers to investigate factors influencing their distribution and status in western Glacier National Park, Montana. We evaluated the association of a suite of abiotic factors (stream width, elevation, gradient, large woody debris density, pool density, August mean stream temperature, reach surface area) with the occurrence (presence or absence) of bull trout and westslope cutthroat trout in 79 stream reaches in five sub-drainages containing glacial lakes. We modeled the occurrence of each species using logistic regression and evaluated competing models using an information theoretic approach. Westslope cutthroat trout were widely distributed (47 of 79 reaches), and there appeared to be no restrictions on their distribution other than physical barriers. Westslope cutthroat trout were most commonly found in relatively warm reaches downstream of lakes and in headwater reaches with large amounts of large woody debris and abundant pools. By contrast, bull trout were infrequently detected (10 of 79 reaches), with 7 of the 10 (70%) detections in sub-drainages that have not been compromised by non-native lake trout (S. namaycush). Bull trout were most often found in cold, low-gradient reaches upstream of glacial lakes. Our results indicate that complex stream habitats in sub-drainages free of non-native species are important to the persistence of native salmonids in western Glacier National Park. Results from this study may help managers monitor and protect important habitats and populations, inform conservation and recovery programs, and guide non-native species suppression efforts in Glacier National Park and elsewhere.

  14. Egg fatty acid composition from lake trout fed two Lake Michigan prey fish species.

    Science.gov (United States)

    Honeyfield, D.C.; Fitzsimons, J.D.; Tillitt, D.E.; Brown, S.B.

    2009-01-01

    We previously demonstrated that there were significant differences in the egg thiamine content in lake trout Salvelinus namaycush fed two Lake Michigan prey fish (alewife Alosa pseudoharengus and bloater Coregonus hoyi). Lake trout fed alewives produced eggs low in thiamine, but it was unknown whether the consumption of alewives affected other nutritionally important components. In this study we investigated the fatty acid composition of lake trout eggs when females were fed diets that resulted in different egg thiamine concentrations. For 2 years, adult lake trout were fed diets consisting of four combinations of captured alewives and bloaters (100% alewives; 65% alewives, 35% bloaters; 35% alewives, 65% bloaters; and 100% bloaters). The alewife fatty acid profile had higher concentrations of arachidonic acid and total omega-6 fatty acids than the bloater profile. The concentrations of four fatty acids (cis-13, 16-docosadienoic, eicosapentaenoic, docosapentaenoic, and docosahexaenoic acids) were higher in bloaters than in alewives. Although six fatty acid components were higher in lake trout eggs in 2001 than in 2000 and eight fatty acids were lower, diet had no effect on any fatty acid concentration measured in lake trout eggs in this study. Based on these results, it appears that egg fatty acid concentrations differ between years but that the egg fatty acid profile does not reflect the alewife-bloater mix in the diet of adults. The essential fatty acid content of lake trout eggs from females fed alewives and bloaters appears to be physiologically regulated and adequate to meet the requirements of developing embryos.

  15. Fish populations in a large group of acid-stressed lakes

    Energy Technology Data Exchange (ETDEWEB)

    Harvey, H H

    1975-01-01

    The purpose of this study was to determine the effects of environmental stress on the number and diversity of fish species in a group of acid-stressed lakes. The study area was the La Cloche Mountains, a series of quartzite ridges covering 1,300 km/sup 2/ along the north shore of Georgian Bay and north channel of Lake Huron. Within these ridges are 173 lakes; 68 of the largest of these made up the study sample. The lakes of the La Cloche Mountains are undergoing rapid acidification. Coincident with this there has been the loss of sport fishes from several lakes. Lakes such as Nellie, Lumsden, O.S.A., Acid and Killarney supported good sport fisheries for the lake trout, (Salvelinus namaycush) for many years, but have ceased to do so in the last 5 to 15 years. Other sport fishes, notably the walleye (Stizostedion vitreum) and smallmouth bass (micropterus dolomieu) have disappeared from some of the La Cloche Lakes. Thus recreational fishing alone could not have been the cause of the change. Beamish (1974) recorded the extreme sparcity of the three remaining fish species in O.S.A. Lake. Many of the lakes of the La Cloche mountains are accessible only with difficulty and little or no information exists for these lakes prior to this study. This precluded simple comparison of these lakes before and during acidification. This lack of historic data determined in part the approach taken in this study; a comparison of the fish communities of a group of lakes differing in degree of acid stress.

  16. Effects of lake trout refuges on lake whitefish and cisco in the Apostle Islands Region of Lake Superior

    Science.gov (United States)

    Zuccarino-Crowe , Chiara M.; Taylor, William W.; Hansen, Michael J.; Seider, Michael J.; Krueger, Charles C.

    2016-01-01

    Lake trout refuges in the Apostle Islands region of Lake Superior are analogous to the concept of marine protected areas. These refuges, established specifically for lake trout (Salvelinus namaycush) and closed to most forms of recreational and commercial fishing, were implicated as one of several management actions leading to successful rehabilitation of Lake Superior lake trout. To investigate the potential significance of Gull Island Shoal and Devils Island Shoal refuges for populations of not only lake trout but also other fish species, relative abundances of lake trout, lake whitefish (Coregonus clupeaformis), and cisco (Coregonus artedi) were compared between areas sampled inside versus outside of refuge boundaries. During 1982–2010, lake trout relative abundance was higher and increased faster inside the refuges, where lake trout fishing was prohibited, than outside the refuges. Over the same period, lake whitefish relative abundance increased faster inside than outside the refuges. Both evaluations provided clear evidence that refuges protected these species. In contrast, trends in relative abundance of cisco, a prey item of lake trout, did not differ significantly between areas inside and outside the refuges. This result did not suggest indirect or cascading refuge effects due to changes in predator levels. Overall, this study highlights the potential of species-specific refuges to benefit other fish species beyond those that were the refuges' original target. Improved understanding of refuge effects on multiple species of Great Lakes fishes can be valuable for developing rationales for refuge establishment and predicting associated fish community-level effects.

  17. Probabilistic measures of climate change vulnerability, adaptation action benefits, and related uncertainty from maximum temperature metric selection

    Science.gov (United States)

    DeWeber, Jefferson T.; Wagner, Tyler

    2018-01-01

    Predictions of the projected changes in species distributions and potential adaptation action benefits can help guide conservation actions. There is substantial uncertainty in projecting species distributions into an unknown future, however, which can undermine confidence in predictions or misdirect conservation actions if not properly considered. Recent studies have shown that the selection of alternative climate metrics describing very different climatic aspects (e.g., mean air temperature vs. mean precipitation) can be a substantial source of projection uncertainty. It is unclear, however, how much projection uncertainty might stem from selecting among highly correlated, ecologically similar climate metrics (e.g., maximum temperature in July, maximum 30‐day temperature) describing the same climatic aspect (e.g., maximum temperatures) known to limit a species’ distribution. It is also unclear how projection uncertainty might propagate into predictions of the potential benefits of adaptation actions that might lessen climate change effects. We provide probabilistic measures of climate change vulnerability, adaptation action benefits, and related uncertainty stemming from the selection of four maximum temperature metrics for brook trout (Salvelinus fontinalis), a cold‐water salmonid of conservation concern in the eastern United States. Projected losses in suitable stream length varied by as much as 20% among alternative maximum temperature metrics for mid‐century climate projections, which was similar to variation among three climate models. Similarly, the regional average predicted increase in brook trout occurrence probability under an adaptation action scenario of full riparian forest restoration varied by as much as .2 among metrics. Our use of Bayesian inference provides probabilistic measures of vulnerability and adaptation action benefits for individual stream reaches that properly address statistical uncertainty and can help guide conservation

  18. Comparing stream-specific to generalized temperature models to guide salmonid management in a changing climate

    Science.gov (United States)

    Andrew K. Carlson,; William W. Taylor,; Hartikainen, Kelsey M.; Dana M. Infante,; Beard, Douglas; Lynch, Abigail

    2017-01-01

    Global climate change is predicted to increase air and stream temperatures and alter thermal habitat suitability for growth and survival of coldwater fishes, including brook charr (Salvelinus fontinalis), brown trout (Salmo trutta), and rainbow trout (Oncorhynchus mykiss). In a changing climate, accurate stream temperature modeling is increasingly important for sustainable salmonid management throughout the world. However, finite resource availability (e.g. funding, personnel) drives a tradeoff between thermal model accuracy and efficiency (i.e. cost-effective applicability at management-relevant spatial extents). Using different projected climate change scenarios, we compared the accuracy and efficiency of stream-specific and generalized (i.e. region-specific) temperature models for coldwater salmonids within and outside the State of Michigan, USA, a region with long-term stream temperature data and productive coldwater fisheries. Projected stream temperature warming between 2016 and 2056 ranged from 0.1 to 3.8 °C in groundwater-dominated streams and 0.2–6.8 °C in surface-runoff dominated systems in the State of Michigan. Despite their generally lower accuracy in predicting exact stream temperatures, generalized models accurately projected salmonid thermal habitat suitability in 82% of groundwater-dominated streams, including those with brook charr (80% accuracy), brown trout (89% accuracy), and rainbow trout (75% accuracy). In contrast, generalized models predicted thermal habitat suitability in runoff-dominated streams with much lower accuracy (54%). These results suggest that, amidst climate change and constraints in resource availability, generalized models are appropriate to forecast thermal conditions in groundwater-dominated streams within and outside Michigan and inform regional-level salmonid management strategies that are practical for coldwater fisheries managers, policy makers, and the public. We recommend fisheries professionals reserve resource

  19. Integrating Traditional and Evolutionary Knowledge in Biodiversity Conservation: a Population Level Case Study

    Directory of Open Access Journals (Sweden)

    Dylan J. Fraser

    2006-12-01

    Full Text Available Despite their dual importance in the assessment of endangered/threatened species, there have been few attempts to integrate traditional ecological knowledge (TEK and evolutionary biology knowledge (EBK at the population level. We contrasted long-term aboriginal TEK with previously obtained EBK in the context of seasonal migratory habits and population biology of a salmonid fish, brook charr, (Salvelinus fontinalis inhabiting a large, remote postglacial lake. Compilation of TEK spanning four decades involved analytical workshops, semidirective interviews, and collaborative fieldwork with local aboriginal informants and fishing guides. We found that TEK complemented EBK of brook charr by providing concordant and additional information about (1 population viability; (2 breeding areas and migration patterns of divergent populations; and (3 the behavioral ecology of populations within feeding areas; all of which may ultimately affect the maintenance of population diversity. Aboriginal concerns related to human pressures on this species, not revealed by EBK, also help to focus future conservation initiatives for divergent populations and to encourage restoration of traditional fishing practices. However, relative to EBK, the relevance of TEK to salmonid biodiversity conservation was evident mainly at a smaller spatial scale, for example, that of individual rivers occupied by populations or certain lake sectors. Nevertheless, EBK was only collected over a 4-yr period, so TEK provided an essential long-term temporal window to evaluate population differences and persistence. We concluded that, despite different conceptual underpinnings, spatially and temporally varying TEK and EBK both contribute to the knowledge base required to achieve sustainability and effective biodiversity conservation planning for a given species. Such integration may be particularly relevant in many isolated regions, where intraspecific diversity can go unrecognized due to sparse

  20. A comparative evaluation of crowding stress on muscle HSP90 and myostatin expression in salmonids

    Science.gov (United States)

    Galt, Nicholas J.; Froehlich, Jacob Michael; McCormick, Stephen; Biga, Peggy R.

    2018-01-01

    Stress is a major factor that contributes to poor production and animal welfare concerns in aquaculture. As such, a thorough understanding of mechanisms involved in the stress response is imperative to developing strategies to mitigate the negative side effects of stressors, including the impact of high stocking densities on growth. The purpose of this study was to determine how the muscle growth inhibitor, myostatin, and the stress-responsive gene HSP90 are regulated in response to crowding stress in rainbow trout (Oncorhynchus mykiss), cutthroat trout (Oncorhynchus clarki), brook trout (Salvelinus fontinalis), and Atlantic salmon (Salmo salar). All species exhibited higher cortisol and glucose levels following the handling stress, indicating physiological response to the treatment. Additionally, all species, except rainbow trout, exhibited higher HSP90 levels in muscle after a 48 h crowding stress. Crowding stress resulted in a decrease of myostatin-1ain brook trout white muscle but not red muscle, while, myostatin-1a and -2a levels increased in white muscle and myostatin-1b levels increased in red muscle in Atlantic salmon. In rainbow trout, no significant changes were detected in either muscle type, but myostatin-1awas upregulated in both white and red skeletal muscle in the closely related cutthroat trout. The variation in response to crowding suggests a complex and species-specific interaction between stress and the muscle gene regulation in these salmonids. Only Atlantic salmon and cutthroat trout exhibited increased muscle myostatin transcription, and also exhibited the largest increase in circulating glucose in response to crowding. These results suggest that species-specific farming practices should be carefully examined in order to optimize low stress culture conditions.

  1. Probabilistic measures of climate change vulnerability, adaptation action benefits, and related uncertainty from maximum temperature metric selection.

    Science.gov (United States)

    DeWeber, Jefferson T; Wagner, Tyler

    2018-06-01

    Predictions of the projected changes in species distributions and potential adaptation action benefits can help guide conservation actions. There is substantial uncertainty in projecting species distributions into an unknown future, however, which can undermine confidence in predictions or misdirect conservation actions if not properly considered. Recent studies have shown that the selection of alternative climate metrics describing very different climatic aspects (e.g., mean air temperature vs. mean precipitation) can be a substantial source of projection uncertainty. It is unclear, however, how much projection uncertainty might stem from selecting among highly correlated, ecologically similar climate metrics (e.g., maximum temperature in July, maximum 30-day temperature) describing the same climatic aspect (e.g., maximum temperatures) known to limit a species' distribution. It is also unclear how projection uncertainty might propagate into predictions of the potential benefits of adaptation actions that might lessen climate change effects. We provide probabilistic measures of climate change vulnerability, adaptation action benefits, and related uncertainty stemming from the selection of four maximum temperature metrics for brook trout (Salvelinus fontinalis), a cold-water salmonid of conservation concern in the eastern United States. Projected losses in suitable stream length varied by as much as 20% among alternative maximum temperature metrics for mid-century climate projections, which was similar to variation among three climate models. Similarly, the regional average predicted increase in brook trout occurrence probability under an adaptation action scenario of full riparian forest restoration varied by as much as .2 among metrics. Our use of Bayesian inference provides probabilistic measures of vulnerability and adaptation action benefits for individual stream reaches that properly address statistical uncertainty and can help guide conservation actions. Our

  2. The developmental transcriptome of contrasting Arctic charr (Salvelinus alpinus morphs [version 2; referees: 1 approved, 2 approved with reservations

    Directory of Open Access Journals (Sweden)

    Johannes Gudbrandsson

    2016-04-01

    Full Text Available Species and populations with parallel evolution of specific traits can help illuminate how predictable adaptations and divergence are at the molecular and developmental level. Following the last glacial period, dwarfism and specialized bottom feeding morphology evolved rapidly in several landlocked Arctic charr Salvelinus alpinus populations in Iceland.   To study the genetic divergence between small benthic morphs and limnetic morphs, we conducted RNA-sequencing charr embryos at four stages in early development. We studied two stocks with contrasting morphologies: the small benthic (SB charr from Lake Thingvallavatn and Holar aquaculture (AC charr. The data reveal significant differences in expression of several biological pathways during charr development. There was also an expression difference between SB- and AC-charr in genes involved in energy metabolism and blood coagulation genes. We confirmed differing expression of five genes in whole embryos with qPCR, including lysozyme and natterin-like which was previously identified as a fish-toxin of a lectin family that may be a putative immunopeptide. We also verified differential expression of 7 genes in the developing head that associated consistently with benthic v.s.limnetic morphology (studied in 4 morphs. Comparison of single nucleotide polymorphism (SNP frequencies reveals extensive genetic differentiation between the SB and AC-charr (~1300 with more than 50% frequency difference. Curiously, three derived alleles in the otherwise conserved 12s and 16s mitochondrial ribosomal RNA genes are found in benthic charr. The data implicate multiple genes and molecular pathways in divergence of small benthic charr and/or the response of aquaculture charr to domestication. Functional, genetic and population genetic studies on more freshwater and anadromous populations are needed to confirm the specific loci and mutations relating to specific ecological traits in Arctic charr.

  3. The developmental transcriptome of contrasting Arctic charr (Salvelinus alpinus morphs [version 3; referees: 1 approved, 2 approved with reservations

    Directory of Open Access Journals (Sweden)

    Johannes Gudbrandsson

    2016-12-01

    Full Text Available Species and populations with parallel evolution of specific traits can help illuminate how predictable adaptations and divergence are at the molecular and developmental level. Following the last glacial period, dwarfism and specialized bottom feeding morphology evolved rapidly in several landlocked Arctic charr Salvelinus alpinus populations in Iceland.   To study the genetic divergence between small benthic morphs and limnetic morphs, we conducted RNA-sequencing charr embryos at four stages in early development. We studied two stocks with contrasting morphologies: the small benthic (SB charr from Lake Thingvallavatn and Holar aquaculture (AC charr. The data reveal significant differences in expression of several biological pathways during charr development. There was also an expression difference between SB- and AC-charr in genes involved in energy metabolism and blood coagulation genes. We confirmed differing expression of five genes in whole embryos with qPCR, including lysozyme and natterin-like which was previously identified as a fish-toxin of a lectin family that may be a putative immunopeptide. We also verified differential expression of 7 genes in the developing head that associated consistently with benthic v.s.limnetic morphology (studied in 4 morphs. Comparison of single nucleotide polymorphism (SNP frequencies reveals extensive genetic differentiation between the SB and AC-charr (~1300 with more than 50% frequency difference. Curiously, three derived alleles in the otherwise conserved 12s and 16s mitochondrial ribosomal RNA genes are found in benthic charr. The data implicate multiple genes and molecular pathways in divergence of small benthic charr and/or the response of aquaculture charr to domestication. Functional, genetic and population genetic studies on more freshwater and anadromous populations are needed to confirm the specific loci and mutations relating to specific ecological traits in Arctic charr.

  4. Pipeline corridors through wetlands -- Impacts on plant communities: Norris Brook Crossing Peabody, Massachusetts

    Energy Technology Data Exchange (ETDEWEB)

    Shem, L.M.; Van Dyke, G.D.; Zimmerman, R.E. [Argonne National Lab., IL (United States)

    1994-12-01

    The goal of the Gas Research Institute Wetland Corridors Program is to document impacts of existing pipelines on the wetlands they traverse. To accomplish this goal, 12 existing wetland crossings were surveyed. These sites varied in elapsed time since pipeline construction, wetland type, pipeline installation techniques, and right-of-way (ROW) management practices. This report presents the results of a survey conducted August 17--19, 1992, at the Norris Brook crossing in the town of Peabody, Essex County, Massachusetts. The pipeline at this site was installed during September and October 1990. A backhoe was used to install the pipeline. The pipe was assembled on the adjacent upland and slid into the trench, after which the backhoe was used again to fill the trench and cover the pipeline. Within two years after pipeline construction, a dense vegetative community, composed predominantly of native perennial species, had become established on the ROW. Compared with adjacent natural areas undisturbed by pipeline installation, there was an increase in purple loosestrife and cattail within the ROW, while large woody species were excluded from the ROW. As a result of the ROW`s presence, habitat diversity, edge-type habitat, and species diversity increased within the site. Crooked-stem aster, Aster prenanthoides (a species on the Massasschusetts list of plants of special concern), occurred in low numbers in the adjacent natural areas and had reinvaded the ROW in low numbers.

  5. A SNP Based Linkage Map of the Arctic Charr (Salvelinus alpinus Genome Provides Insights into the Diploidization Process After Whole Genome Duplication

    Directory of Open Access Journals (Sweden)

    Cameron M. Nugent

    2017-02-01

    Full Text Available Diploidization, which follows whole genome duplication events, does not occur evenly across the genome. In salmonid fishes, certain pairs of homeologous chromosomes preserve tetraploid loci in higher frequencies toward the telomeres due to residual tetrasomic inheritance. Research suggests this occurs only in homeologous pairs where one chromosome arm has undergone a fusion event. We present a linkage map for Arctic charr (Salvelinus alpinus, a salmonid species with relatively fewer chromosome fusions. Genotype by sequencing identified 19,418 SNPs, and a linkage map consisting of 4508 markers was constructed from a subset of high quality SNPs and microsatellite markers that were used to anchor the new map to previous versions. Both male- and female-specific linkage maps contained the expected number of 39 linkage groups. The chromosome type associated with each linkage group was determined, and 10 stable metacentric chromosomes were identified, along with a chromosome polymorphism involving the sex chromosome AC04. Two instances of a weak form of pseudolinkage were detected in the telomeric regions of homeologous chromosome arms in both female and male linkage maps. Chromosome arm homologies within the Atlantic salmon (Salmo salar and rainbow trout (Oncorhynchus mykiss genomes were determined. Paralogous sequence variants (PSVs were identified, and their comparative BLASTn hit locations showed that duplicate markers exist in higher numbers on seven pairs of homeologous arms, previously identified as preserving tetrasomy in salmonid species. Homeologous arm pairs where neither arm has been part of a fusion event in Arctic charr had fewer PSVs, suggesting faster diploidization rates in these regions.

  6. Level II scour analysis for Bridge 24 (MANCUS00070024) on U.S. Route 7, crossing Lye Brook, Manchester, Vermont

    Science.gov (United States)

    Olson, Scott A.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure MANCUS00070024 on U.S. Route 7 crossing Lye Brook, Manchester, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D. The site is in the Taconic section of the New England physiographic province in southwestern Vermont. The 8.13-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the primary surface cover consists of brush and trees. In the study area, Lye Brook has an incised, sinuous channel with a slope of approximately 0.03 ft/ft, an average channel top width of 66 ft and an average bank height of 11 ft. The channel bed material ranges from gravel to boulder with a median grain size (D50) of 90.0 mm (0.295 ft). The geomorphic assessment at the time of the Level I and Level II site visit on August 6, 1996, indicated that the reach was stable. Although, the immediate reach is considered stable, upstream of the bridge the Lye Brook valley is very steep (0.05 ft/ft). Extreme events in a valley this steep may quickly reveal the instability of the channel. In the Flood Insurance Study for the Town of Manchester (Federal Emergency Management Agency, January, 1985), Lye Brook’s overbanks were described as “boulder strewn” after the August 1976 flood. The U.S. Route 7 crossing of Lye Brook is a 28-ft-long, two-lane bridge consisting of one 25-foot concrete span (Vermont Agency of Transportation, written communication, September

  7. Climate change expands the spatial extent and duration of preferred thermal habitat for lake Superior fishes.

    Directory of Open Access Journals (Sweden)

    Timothy J Cline

    Full Text Available Climate change is expected to alter species distributions and habitat suitability across the globe. Understanding these shifting distributions is critical for adaptive resource management. The role of temperature in fish habitat and energetics is well established and can be used to evaluate climate change effects on habitat distributions and food web interactions. Lake Superior water temperatures are rising rapidly in response to climate change and this is likely influencing species distributions and interactions. We use a three-dimensional hydrodynamic model that captures temperature changes in Lake Superior over the last 3 decades to investigate shifts in habitat size and duration of preferred temperatures for four different fishes. We evaluated habitat changes in two native lake trout (Salvelinus namaycush ecotypes, siscowet and lean lake trout, Chinook salmon (Oncorhynchus tshawytscha, and walleye (Sander vitreus. Between 1979 and 2006, days with available preferred thermal habitat increased at a mean rate of 6, 7, and 5 days per decade for lean lake trout, Chinook salmon, and walleye, respectively. Siscowet lake trout lost 3 days per decade. Consequently, preferred habitat spatial extents increased at a rate of 579, 495 and 419 km(2 per year for the lean lake trout, Chinook salmon, and walleye while siscowet lost 161 km(2 per year during the modeled period. Habitat increases could lead to increased growth and production for three of the four fishes. Consequently, greater habitat overlap may intensify interguild competition and food web interactions. Loss of cold-water habitat for siscowet, having the coldest thermal preference, could forecast potential changes from continued warming. Additionally, continued warming may render more suitable conditions for some invasive species.

  8. Seasonally dynamic diel vertical migrations of Mysis diluviana, coregonine fishes, and siscowet lake trout in the pelagia of western Lake Superior

    Science.gov (United States)

    Ahrenstorff, Tyler D.; Hrabik, Thomas R.; Stockwell, Jason D.; Yule, Daniel L.; Sass, Greg G.

    2011-01-01

    Diel vertical migrations are common among many aquatic species and are often associated with changing light levels. The underlying mechanisms are generally attributed to optimizing foraging efficiency or growth rates and avoiding predation risk (μ). The objectives of this study were to (1) assess seasonal and interannual changes in vertical migration patterns of three trophic levels in the Lake Superior pelagic food web and (2) examine the mechanisms underlying the observed variability by using models of foraging, growth, and μ. Our results suggest that the opossum shrimp Mysis diluviana, kiyi Coregonus kiyi, and siscowet lake trout Salvelinus namaycush migrate concurrently during each season, but spring migrations are less extensive than summer and fall migrations. In comparison with M. diluviana, kiyis, and siscowets, the migrations by ciscoes C. artedi were not as deep in the water column during the day, regardless of season. Foraging potential and μ probably drive the movement patterns of M. diluviana, while our modeling results indicate that movements by kiyis and ciscoes are related to foraging opportunity and growth potential and receive a lesser influence from μ. The siscowet is an abundant apex predator in the pelagia of Lake Superior and probably undertakes vertical migrations in the water column to optimize foraging efficiency and growth. The concurrent vertical movement patterns of most species are likely to facilitate nutrient transport in this exceedingly oligotrophic ecosystem, and they demonstrate strong linkages between predators and prey. Fishery management strategies should use an ecosystem approach and should consider how altering the densities of long-lived top predators produces cascading effects on the nutrient cycling and energy flow in lower trophic levels.

  9. Pumpkin seed cake as a fishmeal substitute in fish nutrition: effects on growth performance, morphological traits and fillet colour of two freshwater salmonids and two catfish species.

    Science.gov (United States)

    Greiling, Alexander Michael; Schwarz, Christiane; Gierus, Martin; Rodehutscord, Markus

    2018-06-01

    The objectives of this study were to investigate the digestibility of pumpkin seed cake (PSC) for the rainbow trout, Oncorhynchus mykiss (Walbaum, 1792), and effects on performance and product quality traits of four different fish species when PSC partially replaced fishmeal in extruded diets. A digestibility trial was carried out to determine apparent digestibility coefficients (ADC) for crude protein (CP), ether extract (EE) and gross energy (GE) of PSC fed to rainbow trout. In subsequent growth trials, effects on performance and morphological traits and fillet colour values of four different fish species [rainbow trout; brook trout, Salvelinus fontinalis (Mitchill, 1814); African sharptooth catfish, Clarias gariepinus (Burchell, 1822); and wels catfish, Silurus glanis (Linnaeus, 1758)] were evaluated when 60% of fishmeal protein of a reference diet was replaced by PSC protein (based on digestible CP). Nutrient ADC of PSC were high (CP: 89%, EE: 88% and GE: 84%). No significant effects on growth and only minor effects on fillet colour were detected in the trials. However, replacing fishmeal with PSC at the chosen level affected morphological traits and feed conversion in all four species to different extents. Replacement effects of PSC should be tested at lower levels of inclusion before conclusions are drawn on its suitability in fish diets.

  10. Summer Season Water Temperature Modeling under the Climate Change: Case Study for Fourchue River, Quebec, Canada

    Directory of Open Access Journals (Sweden)

    Jaewon Kwak

    2017-05-01

    Full Text Available It is accepted that human-induced climate change is unavoidable and it will have effects on physical, chemical, and biological properties of aquatic habitats. This will be especially important for cold water fishes such as trout. The objective of this study is to simulate water temperature for future periods under the climate change situations. Future water temperature in the Fourchue River (St-Alexandre-de-Kamouraska, QC, Canada were simulated by the CEQUEAU hydrological and water temperature model, using meteorological inputs from the Coupled Model Intercomparison Project Phase 5 (CMIP5 Global Circulation Models (GCMs with Representative Concentration Pathway (RCP 2.6, 4.5 and 8.5 climate change scenarios. The result of the study indicated that water temperature in June will increase 0.2–0.7 °C and that in September, median water temperature could decrease by 0.2–1.1 °C. The rise in summer water temperature may be favorable to brook trout (Salvelinus fontinalis growth, but several days over the Upper Incipient Lethal Temperature (UILT are also likely to occur. Therefore, flow regulation procedures, including cold water releases from the Morin dam may have to be considered for the Fourchue River.

  11. Level II scour analysis for Bridge 37 (DUXBTH00120037) on Town Highway 12, crossing Ridley Brook, Duxbury, Vermont

    Science.gov (United States)

    Wild, Emily C.; Ivanhoff, Michael A.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure DUXBTH00120037 on Town Highway 12 crossing Ridley Brook, Duxbury, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D. The site is in the Green Mountain section of the New England physiographic province in north central Vermont. The 10.1-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest upstream and downstream of the bridge. In the study area, Ridley Brook has an incised, straight channel with a slope of approximately 0.04 ft/ft, an average channel top width of 67 ft and an average bank height of 9 ft. The channel bed material ranges from gravel to boulders with a median grain size (D50) of 123 mm (0.404 ft). The geomorphic assessment at the time of the Level I and Level II site visit on July 1, 1996, indicated that the reach was stable. The Town Highway 12 crossing of Ridley Brook is a 33-ft-long, two-lane bridge consisting of five 30-ft steel rolled beams (Vermont Agency of Transportation, written communication, October 13, 1995). The opening length of the structure parallel to the bridge face is 30 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 50 degrees to the opening while the measured opening-skew-to-roadway is 20 degrees. A scour hole 2 ft deeper than the mean thalweg depth was observed along the right abutment and downstream

  12. Level II scour analysis for Bridge 17 (LYNDTH00020017) on Town Highway 2, crossing Hawkins Brook, Lyndon, Vermont

    Science.gov (United States)

    Wild, Emily C.; Medalie, Laura

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure LYNDTH00020017 on Town Highway 2 crossing Hawkins Brook, Lyndon, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.The site is in the Green Mountain section of the New England physiographic province in northeastern Vermont. The 7.7-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest on the left and right upstream overbanks. The downstream left and right overbanks are brushland.In the study area, Hawkins Brook has an incised, sinuous channel with a slope of approximately 0.02 ft/ft, an average channel top width of 78 ft and an average bank height of 7.3 ft. The channel bed material ranges from sand to boulder with a median grain size (D50) of 46.6 mm (0.153 ft). The geomorphic assessment at the time of the Level I and Level II site visit on August 4, 1995, indicated that the reach was laterally unstable with the presence of point bars and side bars.The Town Highway 2 crossing of Hawkins Brook is a 49-ft-long, two-lane bridge consisting of a 46-foot steel-stringer span (Vermont Agency of Transportation, written communication, March 27, 1995). The opening length of the structure parallel to the bridge face is 43 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 45 degrees to the opening while the computed opening-skew-to-roadway is zero

  13. Level II scour analysis for Bridge 28 (ROCHTH00370028) on Town Highway 37, crossing Brandon Brook, Rochester, Vermont

    Science.gov (United States)

    Wild, Emily C.; Weber, Matthew A.

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure ROCHTH00370028 on Town Highway 37 crossing Brandon Brook, Rochester, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from VTAOT files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the Green Mountain section of the New England physiographic province in central Vermont. The 8.0-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is pasture on the upstream left overbank although the immediate banks have dense woody vegetation. The upstream right overbank and downstream left and right overbanks are forested. In the study area, the Brandon Brook has an incised, sinuous channel with a slope of approximately 0.01 ft/ft, an average channel top width of 44 ft and an average bank height of 7 ft. The channel bed material ranges from gravel to cobbles with a median grain size (D50) of 84.2 mm (0.276 ft). The geomorphic assessment at the time of the Level I site visit on April 12, 1995 and Level II site visit on July 8, 1996, indicated that the reach was stable. The Town Highway 37 crossing of the Brandon Brook is a 33-ft-long, one-lane bridge consisting of a 31-foot timber-stringer span (Vermont Agency of Transportation, written communication, March 22, 1995). The opening length of the structure parallel to the bridge face is 29.6 ft. The bridge is supported by vertical, timber log cribbing abutments with wingwalls. The channel is skewed approximately 5 degrees to the opening while the computed opening-skew-to-roadway is zero

  14. Investigations of bull trout (Salvelinus confluentus), steelhead trout (Oncorhynchus mykiss), and spring chinook salmon (O. tshawytscha) interactions in Southeast Washington streams. Final report 1992

    International Nuclear Information System (INIS)

    Underwood, K.D.; Martin, S.W.; Schuck, M.L.; Scholz, A.T.

    1995-01-01

    The goal of this two year study was to determine if supplementation with hatchery reared steelhead trout (Oncorhynchus mykiss) and spring chinook salmon (O. tshawytscha) negatively impacted wild native bull trout (Salvelinus confluentus) through competitive interactions. Four streams with varying levels of fish supplementation activity were sampled in Southeast Washington. Tasks performed during this study were population density, relative abundance, microhabitat utilization, habitat availability, diet analysis, bull trout spawning ground surveys, radio telemetry of adult bull trout, and growth analysis. Results indicate that bull trout overlapped geographically with the supplemented species in each of the study streams suggesting competition among species was possible. Within a stream, bull trout and the supplemented species utilized dissimilar microhabitats and microhabitat utilization by each species was the same among streams suggesting that there was no shifts in microhabitat utilization among streams. The diet of bull trout and O. mykiss significantly overlapped in each of the study streams. The stream most intensely supplemented contained bull trout with the slowest growth and the non-supplemented stream contained bull trout with the fastest growth. Conversely, the stream most intensely supplemented contain steelhead with the fastest growth and the non-supplemented stream contained steelhead with the slowest growth. Growth indicated that bull trout may have been negatively impacted from supplementation, although other factors may have contributed. At current population levels, and current habitat quantity and quality, no impacts to bull trout as a result of supplementation with hatchery reared steelhead trout and spring chinook salmon were detected. Project limitations and future research recommendations are discussed

  15. Investigations of Bull Trout (Salvelinus Confluentus), Steelhead Trout (Oncorhynchus Mykiss), and Spring Chinook Salmon (O. Tshawytscha) Interactions in Southeast Washington Streams. Final Report 1992.

    Energy Technology Data Exchange (ETDEWEB)

    Underwood, Keith D.

    1995-01-01

    The goal of this two year study was to determine if supplementation with hatchery reared steelhead trout (Oncorhynchus mykiss) and spring chinook salmon (O. tshawytscha) negatively impacted wild native bull trout (Salvelinus confluentus) through competitive interactions. Four streams with varying levels of fish supplementation activity were sampled in Southeast Washington. Tasks performed during this study were population density, relative abundance, microhabitat utilization, habitat availability, diet analysis, bull trout spawning ground surveys, radio telemetry of adult bull trout, and growth analysis. Results indicate that bull trout overlapped geographically with the supplemented species in each of the study streams suggesting competition among species was possible. Within a stream, bull trout and the supplemented species utilized dissimilar microhabitats and microhabitat utilization by each species was the same among streams suggesting that there was no shifts in microhabitat utilization among streams. The diet of bull trout and O. mykiss significantly overlapped in each of the study streams. The stream most intensely supplemented contained bull trout with the slowest growth and the non-supplemented stream contained bull trout with the fastest growth. Conversely, the stream most intensely supplemented contain steelhead with the fastest growth and the non-supplemented stream contained steelhead with the slowest growth. Growth indicated that bull trout may have been negatively impacted from supplementation, although other factors may have contributed. At current population levels, and current habitat quantity and quality, no impacts to bull trout as a result of supplementation with hatchery reared steelhead trout and spring chinook salmon were detected. Project limitations and future research recommendations are discussed.

  16. Results of the independent radiological verification survey of the lower Sheffield Brook floodplain, Wayne, New Jersey

    International Nuclear Information System (INIS)

    Yalcintas, M.G.; Carrier, R.F.

    1989-05-01

    Prior to 1971, the W.R. Grace Company processed and stored radioactive materials at Wayne, New Jersey, under license to the Atomic Energy Commission. Decontamination of structures and storage of waste materials on the property at the Wayne Interim Storage Site (WISS) took place in 1974. Surveys by the State of New Jersey Department of Environmental Protection and by Oak Ridge Associated Universities for the NRC in 1982 indicated that properties adjacent to the WISS contained surface contamination by radioactive residuals in amounts exceeding those acceptable under US Department of Energy (DOE) remedial action guidelines. At the request of DOE, remedial actions have been conducted by Bechtel National, Inc., to remove radioactive residuals from properties adjacent to the site. It is the policy of DOE to assign an independent verification contractor to ensure the effectiveness of remedial actions performed within the Formerly Utilized Sites Remedial Action Program. This report describes the methods and results of those studies that were conducted by the Measurement Applications and Development Group of the Oak Ridge National Laboratory for the lower Sheffield Brook floodplain west of the WISS. Based upon post-remedial action and verification survey data, it was concluded that residual soil concentrations and gamma levels following excavation and backfilling of the area are within the limits prescribed by DOE radiological guidelines. 12 refs., 6 figs., 8 tabs

  17. Brad Patterson, Tom Brooking, and Jim McAloon, Unpacking the Kist: The Scots in New Zealand. McGill-Queen's Studies in Ethnic History Series, No. 2.33. Montreal: McGill-Queen’s University Press, 2013. Pp. 412. ISBN 978-0-7735-4190-0. CAD $100.00.

    Directory of Open Access Journals (Sweden)

    Seán Gerard Brosnahan

    2014-11-01

    Full Text Available Brad Patterson, Tom Brooking, and Jim McAloon, Unpacking the Kist: The Scots in New Zealand. McGill-Queen's Studies in Ethnic History Series, No. 2.33. Montreal: McGill-Queen’s University Press, 2013. Pp. 412. ISBN 978-0-7735-4190-0. CAD $100.00.

  18. Investigations of bull trout (Salvelinus confluentus), steelhead trout (Oncorhynchus mykiss), and spring chinook salmon (O. tshawytscha) interactions in Southeast Washington streams. Final report 1992; FINAL

    International Nuclear Information System (INIS)

    Underwood, K.D.; Martin, S.W.; Schuck, M.L.; Scholz, A.T.

    1995-01-01

    The goal of this two year study was to determine if supplementation with hatchery reared steelhead trout (Oncorhynchus mykiss) and spring chinook salmon (O. tshawytscha) negatively impacted wild native bull trout (Salvelinus confluentus) through competitive interactions. Four streams with varying levels of fish supplementation activity were sampled in Southeast Washington. Tasks performed during this study were population density, relative abundance, microhabitat utilization, habitat availability, diet analysis, bull trout spawning ground surveys, radio telemetry of adult bull trout, and growth analysis. Results indicate that bull trout overlapped geographically with the supplemented species in each of the study streams suggesting competition among species was possible. Within a stream, bull trout and the supplemented species utilized dissimilar microhabitats and microhabitat utilization by each species was the same among streams suggesting that there was no shifts in microhabitat utilization among streams. The diet of bull trout and O. mykiss significantly overlapped in each of the study streams. The stream most intensely supplemented contained bull trout with the slowest growth and the non-supplemented stream contained bull trout with the fastest growth. Conversely, the stream most intensely supplemented contain steelhead with the fastest growth and the non-supplemented stream contained steelhead with the slowest growth. Growth indicated that bull trout may have been negatively impacted from supplementation, although other factors may have contributed. At current population levels, and current habitat quantity and quality, no impacts to bull trout as a result of supplementation with hatchery reared steelhead trout and spring chinook salmon were detected. Project limitations and future research recommendations are discussed

  19. A regional neural network model for predicting mean daily river water temperature

    Science.gov (United States)

    Wagner, Tyler; DeWeber, Jefferson Tyrell

    2014-01-01

    Water temperature is a fundamental property of river habitat and often a key aspect of river resource management, but measurements to characterize thermal regimes are not available for most streams and rivers. As such, we developed an artificial neural network (ANN) ensemble model to predict mean daily water temperature in 197,402 individual stream reaches during the warm season (May–October) throughout the native range of brook trout Salvelinus fontinalis in the eastern U.S. We compared four models with different groups of predictors to determine how well water temperature could be predicted by climatic, landform, and land cover attributes, and used the median prediction from an ensemble of 100 ANNs as our final prediction for each model. The final model included air temperature, landform attributes and forested land cover and predicted mean daily water temperatures with moderate accuracy as determined by root mean squared error (RMSE) at 886 training sites with data from 1980 to 2009 (RMSE = 1.91 °C). Based on validation at 96 sites (RMSE = 1.82) and separately for data from 2010 (RMSE = 1.93), a year with relatively warmer conditions, the model was able to generalize to new stream reaches and years. The most important predictors were mean daily air temperature, prior 7 day mean air temperature, and network catchment area according to sensitivity analyses. Forest land cover at both riparian and catchment extents had relatively weak but clear negative effects. Predicted daily water temperature averaged for the month of July matched expected spatial trends with cooler temperatures in headwaters and at higher elevations and latitudes. Our ANN ensemble is unique in predicting daily temperatures throughout a large region, while other regional efforts have predicted at relatively coarse time steps. The model may prove a useful tool for predicting water temperatures in sampled and unsampled rivers under current conditions and future projections of climate

  20. A salmonid EST genomic study: genes, duplications, phylogeny and microarrays

    Directory of Open Access Journals (Sweden)

    Brahmbhatt Sonal

    2008-11-01

    Full Text Available Abstract Background Salmonids are of interest because of their relatively recent genome duplication, and their extensive use in wild fisheries and aquaculture. A comprehensive gene list and a comparison of genes in some of the different species provide valuable genomic information for one of the most widely studied groups of fish. Results 298,304 expressed sequence tags (ESTs from Atlantic salmon (69% of the total, 11,664 chinook, 10,813 sockeye, 10,051 brook trout, 10,975 grayling, 8,630 lake whitefish, and 3,624 northern pike ESTs were obtained in this study and have been deposited into the public databases. Contigs were built and putative full-length Atlantic salmon clones have been identified. A database containing ESTs, assemblies, consensus sequences, open reading frames, gene predictions and putative annotation is available. The overall similarity between Atlantic salmon ESTs and those of rainbow trout, chinook, sockeye, brook trout, grayling, lake whitefish, northern pike and rainbow smelt is 93.4, 94.2, 94.6, 94.4, 92.5, 91.7, 89.6, and 86.2% respectively. An analysis of 78 transcript sets show Salmo as a sister group to Oncorhynchus and Salvelinus within Salmoninae, and Thymallinae as a sister group to Salmoninae and Coregoninae within Salmonidae. Extensive gene duplication is consistent with a genome duplication in the common ancestor of salmonids. Using all of the available EST data, a new expanded salmonid cDNA microarray of 32,000 features was created. Cross-species hybridizations to this cDNA microarray indicate that this resource will be useful for studies of all 68 salmonid species. Conclusion An extensive collection and analysis of salmonid RNA putative transcripts indicate that Pacific salmon, Atlantic salmon and charr are 94–96% similar while the more distant whitefish, grayling, pike and smelt are 93, 92, 89 and 86% similar to salmon. The salmonid transcriptome reveals a complex history of gene duplication that is

  1. Spatial and temporal distribution of bull trout (Salvelinus confluentus)-size fish near the floating surface collector in the North Fork Reservoir, Oregon, 2016

    Science.gov (United States)

    Adams, Noah S.; Smith, Collin D.

    2017-06-26

    Acoustic cameras were used to assess the behavior and abundance of bull trout (Salvelinus confluentus)-size fish at the entrance to the North Fork Reservoir juvenile fish floating surface collector (FSC). The purpose of the FSC is to collect downriver migrating juvenile salmonids at the North Fork Dam, and safely route them around the hydroelectric projects. The objective of the acoustic camera component of this study was to assess the behaviors of bull trout-size fish observed near the FSC, and to determine if the presence of bull trout-size fish influenced the collection or abundance of juvenile salmonids. Acoustic cameras were deployed near the surface and floor of the entrance to the FSC. The acoustic camera technology was an informative tool for assessing abundance and spatial and temporal behaviors of bull trout-size fish near the entrance of the FSC. Bull trout-size fish were regularly observed near the entrance, with greater abundances on the deep camera than on the shallow camera. Additionally, greater abundances were observed during the hours of sunlight than were observed during the night. Behavioral differences also were observed at the two depths, with surface fish traveling faster and straighter with more directed movement, and fish observed on the deep camera generally showing more milling behavior. Modeling potential predator-prey interactions and influences using collected passive integrated transponder (PIT) -tagged juvenile salmonids proved largely unpredictable, although these fish provided relevant timing and collection information. Overall, the results indicate that bull trout-size fish are present near the entrance of the FSC, concomitant with juvenile salmonids, and their abundances and behaviors indicate that they may be drawn to the entrance of the FSC because of the abundance of prey-sized fish.

  2. Physiological and biochemical responses of rainbow trout and brook trout exposed to elevated selenium from coal mines

    Energy Technology Data Exchange (ETDEWEB)

    Miller, L.; Rasmussen, J.; Hontela, A. [Lethbridge Univ., Lethbridge, AB (Canada); Palace, V.; Carroll, L. [Fisheries and Oceans Canada, Winnipeg, MB (Canada); Wang, F. [Manitoba Univ., Winnipeg, MB (Canada)

    2009-07-01

    Since selenium (Se) is an element that bioaccumulates, anthropogenic activities in areas that already have high Se in parent rock can result in an increase in Se in aquatic systems. Se causes reproductive deformities in rainbow trout (RT), at lower concentrations than for brook trout (BT). This presentation reported on a study conducted at coal mines in northeastern Alberta in which juvenile hatchery-reared RT and BT were stocked into 2 reference and 2 Se contaminated end pit lakes. Fish were sampled at 0, 6, and 12 months after stocking, and will be sampled at 18 and 24 months. Water Se levels were found to be high in contaminated lakes. Selenite, the more toxic form of Se, was the highest in Pit C4. Liver glutathione levels were similar in the 2 fish species, and cellular damage in the fish was beginning to increase. At 6 months, liver glycogen levels were higher in the BT than in the RT, but no tangible site specific patterns emerged. At all sites except Pit C4, the RT were in better condition than BT. BT from Pit C4 also had a lower ability to secrete cortisol, suggesting that the elevated selenite levels may negatively affect fish. Se accumulation results from 28 fish sampled at 12 months were also discussed.

  3. Effects of acid-sulfate weathering and cyanide-containing gold tailings on the transport and fate of mercury and other metals in Gossan Creek: Murray Brook mine, New Brunswick, Canada

    Energy Technology Data Exchange (ETDEWEB)

    Al, Tom A. [Department of Geology and Canadian Rivers Institute, University of New Brunswick, Fredericton, New Brunswick, E3B 5A3 (Canada)]. E-mail: tal@unb.ca; Leybourne, Matthew I. [Department of Geosciences, University of Texas at Dallas, Box 830688 Richardson, TX 75083-0688 (United States); Maprani, Antu C. [Department of Geology and Canadian Rivers Institute, University of New Brunswick, Fredericton, New Brunswick, E3B 5A3 (Canada); MacQuarrie, Kerry T. [Department of Civil Engineering and Canadian Rivers Institute, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 5A3 (Canada); Dalziel, John A. [Fisheries and Oceans Canada, Marine Chemistry Section, Bedford Institute of Oceanography, Dartmouth, Nova Scotia, B2Y 4A2 (Canada); Fox, Don [New Brunswick Department of Environment and Local Government, Marysville Place 20 McGloin Street, Fredericton, New Brunswick, E3A 5T8 (Canada); Yeats, Phillip A. [Fisheries and Oceans Canada, Marine Chemistry Section, Bedford Institute of Oceanography, Dartmouth, Nova Scotia, B2Y 4A2 (Canada)

    2006-11-15

    Gossan Creek, a headwater stream in the SE Upsalquitch River watershed in New Brunswick, Canada, contains elevated concentrations of total Hg (Hg{sub T} up to 60 {mu}g/L). Aqueous geochemical investigations of the shallow groundwater at the headwaters of the creek confirm that the source of Hg is a contaminated groundwater plume (neutral pH with Hg and Cl concentrations up to 150 {mu}g/L and 20 mg/L, respectively), originating from the Murray Brook mine tailings, that discharges at the headwaters of the creek. The discharge area of the contaminant plume was partially delineated based on elevated pH and Cl concentrations in the groundwater. The local groundwater outside of the plume contains much lower concentrations of Hg and Cl (<0.1 {mu}g/L and 3.8 mg/L, respectively) and displays the chemical characteristics of an acid-sulfate weathering system, with low pH (4.1-5.5) and elevated concentrations of Cu, Zn, Pb and SO{sub 4} (up to 5400 {mu}g Cu/L, 8700 {mu}g Zn/L, 70 {mu}g Pb/L and 330 mg SO{sub 4}/L), derived from oxidation of sulfide minerals in the Murray Brook volcanogenic massive sulfide deposit and surrounding bedrock. The Hg{sub T} mass loads measured at various hydrologic control points along the stream system indicate that 95-99% of the dissolved Hg{sub T} is attenuated in the first 3-4 km from the source. Analyses of creek bed sediments for Au, Ag, Cu, Zn, Pb and Hg indicate that these metals have partitioned strongly to the sediments. Mineralogical investigations of the contaminated sediments using analytical scanning electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM), reveal discrete particles (<1-2 {mu}m) of metacinnabar (HgS), mixed Au-Ag-Hg amalgam, Cu sulfide and Ag sulfide.

  4. Level II scour analysis for Bridge 36 (DUXBTH00040036) on Town Highway 4, crossing Crossett Brook, Duxbury, Vermont

    Science.gov (United States)

    Wild, Emily C.; Degnan, James R.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure DUXBTH00040036 on Town Highway 4 crossing the Crossett Brook, Duxbury, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.The site is in the Green Mountain section of the New England physiographic province in north-central Vermont. The 4.9-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover on the upstream left overbank is pasture. The upstream and downstream right overbanks are forested. The downstream left overbank is brushland, while the immediate banks have dense woody vegetation.In the study area, the Crossett Brook has an incised, sinuous channel with a slope of approximately 0.006 ft/ft, an average channel top width of 55 ft and an average bank height of 9 ft. The channel bed material ranges from gravel to bedrock with a median grain size (D50) of 51.6 mm (0.169 ft). The geomorphic assessment at the time of the Level I and Level II site visit on July 1, 1996, indicated that the reach was stable.The Town Highway 4 crossing of the Crossett Brook is a 29-ft-long, two-lane bridge consisting of a 26-foot concrete slab span (Vermont Agency of Transportation, written communication, October 13, 1995). The opening length of the structure parallel to the bridge face is 26 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 35 degrees to the opening while

  5. Level II scour analysis for Bridge 8 (NEWFTH00010008) on Town Highway 1, crossing Wardsboro Brook, Newfane, Vermont

    Science.gov (United States)

    Wild, Emily C.; Degnan, James

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure NEWFTH00010008 on Town Highway 1 crossing Wardsboro Brook, Newfane, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (Federal Highway Administration, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the New England Upland section of the New England physiographic province in southestern Vermont. The 6.91-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest on the upstream right overbank and downstream left and right overbanks. The surface cover on the upstream left overbank is pasture. In the study area, Wardsboro Brook has an incised, sinuous channel with a slope of approximately 0.02 ft/ft, an average channel top width of 63 ft and an average bank height of 9 ft. The channel bed material ranges from gravel to boulders with a median grain size (D50) of 95.4 mm (0.313 ft). The geomorphic assessment at the time of the Level I and Level II site visit on August 21, 1996, indicated that the reach was stable. The Town Highway 1 crossing of the Wardsboro Brook is a 32-ft-long, two-lane bridge consisting of a 26-foot concrete tee-beam span (Vermont Agency of Transportation, written communication, April 6, 1995). The opening length of the structure parallel to the bridge face is 26.7 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 45 degrees to the computed opening while the openingskew-to-roadway is 45 degrees

  6. Level II scour analysis for Bridge 31 (JERITH00350031) on Town Highway 35, crossing Mill Brook, Jericho, Vermont

    Science.gov (United States)

    Wild, Emily C.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure JERITH00350031 on Town Highway 35 crossing Mill Brook, Jericho, Vermont (figures 1– 8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gathered from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D. The site is in the Green Mountain section of the New England physiographic province and the Champlain section of the St. Lawrence physiographic province in northwestern Vermont. The 15.7-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest upstream of the bridge. The downstream left overbank is pasture. The downstream right overbank is brushland. In the study area, the Mill Brook has an incised, sinuous channel with a slope of approximately 0.02 ft/ft, an average channel top width of 117 ft and an average bank height of 11 ft. The channel bed material ranges from gravel to boulders with a median grain size (D50) of 81.1 mm (0.266 ft). The geomorphic assessment at the time of the Level I and Level II site visit on July 3, 1996, indicated that the reach was laterally unstable. The Town Highway 35 crossing of the Mill Brook is a 53-ft-long, one-lane bridge consisting of a 50-foot steel-beam span with a wooden deck (Vermont Agency of Transportation, written communication, November 30, 1995). The opening length of the structure parallel to the bridge face is 48 ft. The bridge is supported by a vertical, concrete abutment with wingwalls on the left. On the right, the abutment and wingwalls

  7. Level II scour analysis for Bridge 13 (LINCTH00010013) on Town Highway 1, crossing Cota Brook, Lincoln, Vermont

    Science.gov (United States)

    Wild, Emily C.

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure LINCTH00010013 on Town Highway 1 crossing Cota Brook, Lincoln, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the Green Mountain section of the New England physiographic province in west-central Vermont. The 3.0-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest along the upstream right bank and brushland along the upstream left bank. Downstream of the bridge, the surface cover is pasture along the left and right banks. In the study area, Cota Brook has an sinuous channel with a slope of approximately 0.01 ft/ ft, an average channel top width of 30 ft and an average bank height of 2 ft. The channel bed material ranges from sand to cobble with a median grain size (D50) of 34.7 mm (0.114 ft). The geomorphic assessment at the time of the Level I and Level II site visit on June 10, 1996, indicated that the reach was laterally unstable due to cut-banks and wide, vegetated point bars upstream and downstream of the bridge. The Town Highway 1 crossing of Cota Brook is a 38-ft-long, two-lane bridge consisting of a 36-foot steel-stringer span (Vermont Agency of Transportation, written communication, December 14, 1995). The opening length of the structure parallel to the bridge face is 34.4 ft. The bridge is supported by vertical, concrete abutments. The channel is skewed approximately 15 degrees to the opening while

  8. Level II scour analysis for Bridge 13 (SHARTH00040013) on Town Highway 4, crossing Broad Brook, Sharon, Vermont

    Science.gov (United States)

    Wild, Emily C.; Weber, Matthew A.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure SHARTH00040013 on Town Highway 4 crossing Broad Brook, Sharon, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.The site is in the New England Upland section of the New England physiographic province in central Vermont. The 16.6-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is brushland on the downstream left overbank and row crops on the right overbank, while the immediate banks have dense woody vegetation. Upstream of the bridge, the overbanks are forested.In the study area, Broad Brook has an incised, sinuous channel with a slope of approximately 0.02 ft/ft, an average channel top width of 69 ft and an average bank height of 5 ft. The channel bed material ranges from sand to boulder with a median grain size (D50) of 112 mm (0.369 ft). The geomorphic assessment at the time of the Level I site visit on April 11, 1995 and Level II site visit on July 23, 1996, indicated that the reach was stable.The Town Highway 4 crossing of Broad Brook is a 34-ft-long, two-lane bridge consisting of one 31-foot concrete tee beam span (Vermont Agency of Transportation, written communication, March 23, 1995). The opening length of the structure parallel to the bridge face is 30.1 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 10 degrees to the opening while

  9. Genetic diversity of wild and hatchery lake trout populations: Relevance for management and restoration in the Great Lakes

    Science.gov (United States)

    Page, K.S.; Scribner, K.T.; Burnham-Curtis, M.

    2004-01-01

    The biological diversity of lake trout Salvelinus namaycush in the upper Great Lakes was historically high, consisting of many recognizable morphological types and discrete spawning populations. During the 1950s and 1960s, lake trout populations were extirpated from much of the Great Lakes primarily as a result of overfishing and predation by the parasitic sea lamprey Petromyzon marinus. Investigations of how genetic diversity is partitioned among remnant wild lake trout populations and hatchery broodstocks have been advocated to guide lake trout management and conservation planning. Using microsatellite genetic markers, we estimated measures of genetic diversity and the apportionment of genetic variance among 6 hatchery broodstocks and 10 wild populations representing three morphotypes (lean, humper, and siscowet). Analyses revealed that different hatchery broodstocks and wild populations contributed disproportionally to the total levels of genetic diversity. The genetic affinities of hatchery lake trout reflected the lake basins of origin of the wild source populations. The variance in allele frequency over all sampled extant wild populations was apportioned primarily on the basis of morphotype (??MT = 0.029) and secondarily among geographically dispersed populations within each morphotype (??ST = 0.024). The findings suggest that the genetic divergence reflected in recognized morphotypes and the associated ecological and physiological specialization occurred prior to the partitioning of large proglacial lakes into the Great Lakes or as a consequence of higher contemporary levels of gene flow within than among morphotypes. Information on the relative contributions of different broodstocks to total gene diversity within the regional hatchery program can be used to prioritize the broodstocks to be retained and to guide future stocking strategies. The findings highlight the importance of ecological and phenotypic diversity in Great Lakes fish communities and

  10. Boll weevil eradication: a model for sea lamprey control?

    Science.gov (United States)

    Smith, James W.; Swink, William D.

    2003-01-01

    Invasions of boll weevil (Anthonomus grandis) into the United States and sea lamprey (Petromyzon marinus) into the Great Lakes were similar in many ways. Important species (American cotton, Gossypium hirsutum, and lake trout, Salvelinus namaycush) and the industries they supported were negatively affected. Initial control efforts were unsuccessful until pesticides and application technologies were developed. For boll weevils, controls relying on pesticides evolved into an integrated program that included recommended farming practices and poisoned baits. However, the discovery of a boll weevil sex pheromone in 1964 allowed adoption of an ongoing program of eradication. Despite opposition over concept and cost, insecticides, pheromone traps, poisoned baits, and approved farming practices were used to eradicate boll weevils from Virginia, North Carolina, South Carolina, Georgia, Florida, and Alabama by 1999. Using the working back approach along the path of the original invasion, eradication was nearly completed by 2002 in Mississippi and eradication programs were underway in Arkansas, Tennessee, Oklahoma, Louisiana, and parts of Texas. Insecticide use for cotton production decreased 50 to 90%, and cotton yields and farm income increased an average of 78 kg/ha and $190 U.S./ha in areas where boll weevils were eradicated. For sea lampreys, integrated management uses lampricides, barriers to migration, trapping, and release of sterilized males. Although sea lamprey eradication is not currently feasible, recent research on larval and sex pheromones might provide the tools to make it possible. A successful eradication program for sea lampreys starting in Lake Superior and expanding to the lower Great Lakes would ultimately provide huge ecological and economic benefits by eliminating lampricide applications, removing barriers that block teleost fishes, and facilitating the recovery of lake trout. Should the opportunity arise, the concept of sea lamprey eradication should

  11. Effectiveness of a refuge for Lake Trout in Western Lake Superior II: Simulation of future performance

    Science.gov (United States)

    Akins, Andrea L; Hansen, Michael J.; Seider, Michael J.

    2015-01-01

    Historically, Lake Superior supported one of the largest and most diverse Lake Trout Salvelinus namaycush fisheries in the Laurentian Great Lakes, but Lake Trout stocks collapsed due to excessive fishery exploitation and predation by Sea Lampreys Petromyzon marinus. Lake Trout stocking, Sea Lamprey control, and fishery regulations, including a refuge encompassing Gull Island Shoal (Apostle Islands region), were used to enable recovery of Lake Trout stocks that used this historically important spawning shoal. Our objective was to determine whether future sustainability of Lake Trout stocks will depend on the presence of the Gull Island Shoal Refuge. We constructed a stochastic age-structured simulation model to assess the effect of maintaining the refuge as a harvest management tool versus removing the refuge. In general, median abundances of age-4, age-4 and older (age-4+), and age-8+ fish collapsed at lower instantaneous fishing mortality rates (F) when the refuge was removed than when the refuge was maintained. With the refuge in place, the F that resulted in collapse depended on the rate of movement into and out of the refuge. Too many fish stayed in the refuge when movement was low (0–2%), and too many fish became vulnerable to fishing when movement was high (≥22%); thus, the refuge was more effective at intermediate rates of movement (10–11%). With the refuge in place, extinction did not occur at any simulated level of F, whereas refuge removal led to extinction at all combinations of commercial F and recreational F. Our results indicate that the Lake Trout population would be sustained by the refuge at all simulated F-values, whereas removal of the refuge would risk population collapse at much lower F (0.700–0.744). Therefore, the Gull Island Shoal Refuge is needed to sustain the Lake Trout population in eastern Wisconsin waters of Lake Superior.

  12. Use of a seismic air gun to reduce survival of nonnative lake trout embryos: A tool for conservation?

    Science.gov (United States)

    Cox, B.S.; Dux, A.M.; Quist, M.C.; Guy, C.S.

    2012-01-01

    The detrimental impacts of nonnative lake trout Salvelinus namaycush in the western USA have prompted natural resource management agencies in several states to implement lake trout suppression programs. Currently, these programs rely on mechanical removal methods (i.e., gill nets, trap nets, and angling) to capture subadult and adult lake trout. We conducted a study to explore the potential for using high-intensity sound from a relatively small (655.5 cm3 [40 in3]) seismic air gun to reduce survival of lake trout embryos. Lake trout embryos at multiple stages of development were exposed to a single discharge of the seismic air gun at two depths (5 and 15 m) and at two distances from the air gun (0.1 and 2.7 m). Control groups for each developmental stage, distance, and depth were treated identically except that the air gun was not discharged. Mortality in lake trout embryos treated at 0.1 m from the air gun was 100% at 74 daily temperature units in degrees Celsius (TU°C) at both depths. Median mortality in lake trout embryos treated at 0.1 m from the air gun at 207 TU°C (93%) and 267 °C (78%) appeared to be higher than that of controls (49% and 48%, respectively) at 15-m depth. Among the four lake trout developmental stages, exposure to the air gun at 0.1 m resulted in acute mortality up to 60% greater than that of controls. Mortality at a distance of 2.7 m did not appear to differ from that of controls at any developmental stage or at either depth. Our results indicate that seismic air guns have potential as an alternative tool for controlling nonnative lake trout, but further investigation is warranted.

  13. Biotransformation of polychlorinated biphenyls (PCBs) and bioformation of hydroxylated PCBs in fish

    Energy Technology Data Exchange (ETDEWEB)

    Buckman, Andrea H. [Department of Environmental Biology, University of Guelph, Guelph, Ont., N1G 2W1 (Canada); National Waters Research Institute, Environment Canada, Burlington, Ont., Canada L7R 4A6 (Canada); Wong, Charles S. [Department of Chemistry, University of Alberta, Edmonton, Alta. (Canada); Chow, Elaine A. [Department of Chemistry, University of Alberta, Edmonton, Alta. (Canada); Brown, Scott B. [National Waters Research Institute, Environment Canada, Burlington, Ont., L7R 4A6 (Canada); Solomon, Keith R. [Department of Environmental Biology, University of Guelph, Guelph, Ont., N1G 2W1 (Canada); Fisk, Aaron T. [Warnell School of Forest Resources, University of Georgia, Athens, GA 30602-2152 (United States)]. E-mail: afisk@smokey.forestry.uga.edu

    2006-06-15

    Hydroxylated PCBs (OH-PCBs) are a class of organic contaminants that have been found recently in the plasma of Great Lakes fish, the source of which is either bioformation from PCBs or accumulation from the environment. To address the potential for fish to biotransform PCBs and bioform OH-PCBs juvenile rainbow trout (Oncorhynchus mykiss; {approx}80 g) were exposed to dietary concentrations of an environmentally relevant mixture of PCBs. Eight OH-PCBs were found in the plasma of rainbow trout after 30 days of exposure to the PCBs, the relative pattern of which was similar to those observed in wild lake trout (Salvelinus namaycush) from Lake Ontario. Hydroxylated-PCBs were not found (detection limit 0.02 pg/g) in the food or control (not PCB-exposed) fish. A curvilinear log t {sub 1/2}-log K {sub ow} relationship for recalcitrant PCBs was found, similar to previously reported relationships, although t {sub 1/2} values were longer and shorter than studies using smaller fish or cooler temperatures, respectively. A number of PCB congeners fell below the log t {sub 1/2}-log K {sub ow} relationship providing the first estimates of non-chiral PCB biotransformation rates in fish. Enantioselective degradation of the chiral congeners PCBs 91 and 136, also indicated biotransformation. Biotransformation of PCBs was structure-dependent with greater biotransformation of PCBs with vicinal hydrogen atoms in the meta/para positions, suggesting CYP 2B-like biotransformation. Other chiral congeners with a meta/para substitution pattern showed no enantioselective degradation but were biotransformed based on the log t {sub 1/2}-log K {sub ow} relationship. The results of this study demonstrate that laboratory held rainbow trout can biotransform a number of PCB congeners and that bioformation is likely an important source of OH-PCBs in wild salmonids of the Great Lakes.

  14. Biotransformation of polychlorinated biphenyls (PCBs) and bioformation of hydroxylated PCBs in fish

    International Nuclear Information System (INIS)

    Buckman, Andrea H.; Wong, Charles S.; Chow, Elaine A.; Brown, Scott B.; Solomon, Keith R.; Fisk, Aaron T.

    2006-01-01

    Hydroxylated PCBs (OH-PCBs) are a class of organic contaminants that have been found recently in the plasma of Great Lakes fish, the source of which is either bioformation from PCBs or accumulation from the environment. To address the potential for fish to biotransform PCBs and bioform OH-PCBs juvenile rainbow trout (Oncorhynchus mykiss; ∼80 g) were exposed to dietary concentrations of an environmentally relevant mixture of PCBs. Eight OH-PCBs were found in the plasma of rainbow trout after 30 days of exposure to the PCBs, the relative pattern of which was similar to those observed in wild lake trout (Salvelinus namaycush) from Lake Ontario. Hydroxylated-PCBs were not found (detection limit 0.02 pg/g) in the food or control (not PCB-exposed) fish. A curvilinear log t 1/2 -log K ow relationship for recalcitrant PCBs was found, similar to previously reported relationships, although t 1/2 values were longer and shorter than studies using smaller fish or cooler temperatures, respectively. A number of PCB congeners fell below the log t 1/2 -log K ow relationship providing the first estimates of non-chiral PCB biotransformation rates in fish. Enantioselective degradation of the chiral congeners PCBs 91 and 136, also indicated biotransformation. Biotransformation of PCBs was structure-dependent with greater biotransformation of PCBs with vicinal hydrogen atoms in the meta/para positions, suggesting CYP 2B-like biotransformation. Other chiral congeners with a meta/para substitution pattern showed no enantioselective degradation but were biotransformed based on the log t 1/2 -log K ow relationship. The results of this study demonstrate that laboratory held rainbow trout can biotransform a number of PCB congeners and that bioformation is likely an important source of OH-PCBs in wild salmonids of the Great Lakes

  15. Ecological factors affecting Rainbow Smelt recruitment in the main basin of Lake Huron, 1976-2010

    Science.gov (United States)

    O'Brien, Timothy P.; Taylor, William W.; Roseman, Edward F.; Madenjian, Charles P.; Riley, Stephen C.

    2014-01-01

    Rainbow Smelt Osmerus mordax are native to northeastern Atlantic and Pacific–Arctic drainages and have been widely introduced throughout North America. In the Great Lakes region, Rainbow Smelt are known predators and competitors of native fish and a primary prey species in pelagic food webs. Despite their widespread distribution, importance as a prey species, and potential to negatively interact with native fish species, there is limited information concerning stock–recruitment relationships for Rainbow Smelt. To better understand recruitment mechanisms, we evaluated potential ecological factors determining recruitment dynamics for Rainbow Smelt in Lake Huron using data from bottom trawl catches. We specifically evaluated influence of stock size, environmental factors (water temperature, lake levels, and precipitation), and salmonine predation on the production of age-0 recruits from 1976 to 2010. Rainbow Smelt recruitment was negatively related to stock size exceeding 10 kg/ha, indicating that compensatory, density-dependent mortality from cannibalism or intraspecific competition was an important factor related to the production of age-0 recruits. Recruitment was positively related to spring precipitation suggesting that the amount of stream-spawning habitat as determined by precipitation was important for the production of strong Rainbow Smelt recruitment. Additionally, density of age-0 Rainbow Smelt was positively related to Lake Trout Salvelinus namaycush abundance. However, spawning stock biomass of Rainbow Smelt, which declined substantially from 1989 to 2010, was negatively associated with Lake Trout catch per effort suggesting predation was an important factor related to the decline of age-2 and older Rainbow Smelt in Lake Huron. As such, we found that recruitment of Rainbow Smelt in Lake Huron was regulated by competition with or cannibalism by older conspecifics, spring precipitation influencing stream spawning habitats, and predation by Lake Trout on

  16. Effect of artificial sunlight on the retention of external calcein marks on lake trout

    Science.gov (United States)

    Honeyfield, D.C.; Kehler, T.; Fletcher, J.W.; Mohler, J.W.

    2008-01-01

    When choosing a fish marking technique to address fishery related questions, it is important to consider factors that affect mark retention. Calcein, a chemical marking agent, is under investigation for potential use on fish. Two laboratory trials were conducted with calcein-marked lake trout Salvelinus namaycush to determine the effect of artificial sunlight on calcein mark intensity. In trial 1, fish exposed to 18,000 lx for 7 d lost 90% or more of the calcein mark intensity (relative to the colorimetric key, mg/L) on the head, body, ventral region, and pectoral fins relative to mark intensity in fish that were maintained in darkness. In trial 2, light intensity was reduced 2.5-3.0-fold. After 7 d of light exposure, calcein mark intensity on the head was reduced by 40-45% relative to mark intensity in fish that were held in darkness; by day 14, calcein mark intensity on the head was reduced by 55-60% relative to that of dark-treated fish. No further decline was observed in light-exposed fish, and head mark intensity values did not differ among days 14, 21, and 28 for this treatment group. Of the four areas evaluated, the head and pectoral fin were more easily read using a colorimetric key than the lateral or ventral regions of the fish. The concentration of calcein spotted on filter paper to devise the colorimetric key ranged from 1 to 100 mg/L. A difference of approximately 7 mg/L in apparent calcein mark intensity means for the head region could be detected using the colorimetric key. These trials showed that calcein mark intensity on lake trout declined when fish were exposed to artificial sunlight, and the use of a colorimetric key improved the objectivity of calcein mark intensity assessment.

  17. A Lota lota consumption: Trophic dynamics of nonnative Burbot in a valuable sport fishery

    Science.gov (United States)

    Klobucar, Stephen L.; Saunders, W. Carl; Budy, Phaedra

    2016-01-01

    Unintentional and illegal introductions of species disrupt food webs and threaten the success of managed sport fisheries. Although many populations of Burbot Lota lota are declining in the species’ native range, a nonnative population recently expanded into Flaming Gorge Reservoir (FGR), Wyoming–Utah, and threatens to disrupt predator–prey interactions within this popular sport fishery. To determine potential impacts on sport fishes, especially trophy Lake Trout Salvelinus namaycush, we assessed the relative abundance of Burbot and quantified the potential trophic or food web impacts of this population by using diet, stable isotope, and bioenergetic analyses. We did not detect a significant potential for food resource competition between Burbot and Lake Trout (Schoener’s overlap index = 0.13), but overall consumption by Burbot likely affects other sport fishes, as indicated by our analyses of trophic niche space. Diet analyses suggested that crayfish were important diet items across time (89.3% of prey by weight in autumn; 49.4% in winter) and across Burbot size-classes (small: 77.5% of prey by weight; medium: 76.6%; large: 39.7%). However, overall consumption by Burbot increases as water temperatures cool, and fish consumption by Burbot in FGR was observed to increase during winter. Specifically, large Burbot consumed more salmonids, and we estimated (bioenergetically) that up to 70% of growth occurred in late autumn and winter. Further, our population-wide consumption estimates indicated that Burbot could consume up to double the biomass of Rainbow Trout Oncorhynchus mykiss stocked annually (>1.3 × 105 kg; >1 million individuals) into FGR. Overall, we provide some of the first information regarding Burbot trophic interactions outside of the species’ native range; these findings can help to inform the management of sport fisheries if Burbot range expansion occurs elsewhere.

  18. Decline of shortjaw cisco in Lake Superior: the role of overfishing and risk of extinction

    Science.gov (United States)

    Bronte, Charles R.; Hoff, Michael H.; Gorman, Owen T.; Thogmartin, Wayne E.; Schneeberger, Philip J.; Todd, Thomas N.

    2010-01-01

    Recent reviews have further documented the decline of the shortjaw cisco Coregonus zenithicus in Lake Superior. This fish was the most abundant deepwater cisco species in Lake Superior in the early 1920s but presently makes up less than 1% of all deepwater ciscoes (i.e., including shortjaw cisco, bloater C. hoyi, and kiyi C. kiyi) captured in biological surveys. Directed overfishing of deepwater cisco species during the 1930s and again during the mid-1960s and 1970s has been suggested as the cause of the shortjaw cisco's demise. In this paper, we re-examined the overfishing hypothesis by using historical and recent survey data to estimate the proportion of the historical commercial fishery landings that comprised shortjaw ciscoes. We developed time series of estimated harvest and relative abundance for all statistical districts in Michigan waters of Lake Superior during 1929–1996, for which aggregate catch and effort data were available but not previously examined. The spatial distribution of the fishery and the relationships of catch to fishing effort were examined for evidence of overfishing. Our analysis suggested that directed overfishing was probably not the cause of shortjaw cisco demise, as this species appeared to be declining in all statistical districts regardless of the intensity of the fishery. A count-based population viability analysis indicated that quasi-extinction of the shortjaw cisco is highly probable in the near future. We propose an alternative hypothesis based on the decline of Lake Superior's keystone predator, the lake trout Salvelinus namaycush, which resulted in an expansion of the population of its principal prey, the cisco C. artedi, due to release from predation pressure. Competitive or predation interactions between the cisco and shortjaw cisco may be more likely explanations for the demise of the latter species.

  19. Comparison of three nonlinear models to describe long-term tag shedding by lake trout

    Science.gov (United States)

    Fabrizio, Mary C.; Swanson, Bruce L.; Schram, Stephen T.; Hoff, Michael H.

    1996-01-01

    We estimated long-term tag-shedding rates for lake trout Salvelinus namaycush using two existing models and a model we developed to account for the observed permanence of some tags. Because tag design changed over the course of the study, we examined tag-shedding rates for three types of numbered anchor tags (Floy tags FD-67, FD-67C, and FD-68BC) and an unprinted anchor tag (FD-67F). Lake trout from the Gull Island Shoal region, Lake Superior, were double-tagged, and subsequent recaptures were monitored in annual surveys conducted from 1974 to 1992. We modeled tag-shedding rates, using time at liberty and probabilities of tag shedding estimated from fish released in 1974 and 1978–1983 and later recaptured. Long-term shedding of numbered anchor tags in lake trout was best described by a nonlinear model with two parameters: an instantaneous tag-shedding rate and a constant representing the proportion of tags that were never shed. Although our estimates of annual shedding rates varied with tag type (0.300 for FD-67, 0.441 for FD-67C, and 0.656 for FD-68BC), differences were not significant. About 36% of tags remained permanently affixed to the fish. Of the numbered tags that were shed (about 64%), two mechanisms contributed to tag loss: disintegration and dislodgment. Tags from about 11% of recaptured fish had disintegrated, but most tags were dislodged. Unprinted tags were shed at a significant but low rate immediately after release, but the long-term, annual shedding rate of these tags was only 0.013. Compared with unprinted tags, numbered tags dislodged at higher annual rates; we hypothesized that this was due to the greater frictional drag associated with the larger cross-sectional area of numbered tags.

  20. Patterns of δ13C and δ15N in wolverine Gulo gulo tissues from the Brooks Range, Alaska

    Directory of Open Access Journals (Sweden)

    Fredrik DALERUM

    2009-06-01

    Full Text Available Knowledge of carnivore diets is essential to understand how carnivore populations respond demographically to variations in prey abundance. Analysis of stable isotopes is a useful complement to traditional methods of analyzing carnivore diets. We used data on d13C and d15N in wolverine tissues to investigate patterns of seasonal and annual diet variation in a wolverine Gulo gulo population in the western Brooks Range, Alaska, USA. The stable isotope ratios in wolverine tissues generally reflected that of terrestrial carnivores, corroborating previous diet studies on wolverines. We also found variation in d13C and d15N both between muscle samples collected over several years and between tissues with different assimilation rates, even after correcting for isotopic fractionation. This suggests both annual and seasonal diet variation. Our results indicate that data on d13C and d15N holds promise for qualitative assessments of wolverine diet changes over time. Such temporal variation may be important indicators of ecological responses to environmental perturbations, and we suggest that more refined studies of stable isotopes may be an important tool when studying temporal change in diets of wolverines and similar carnivores [Current Zoology 55(3: 188–192, 2009].

  1. Assessing Thermally Stressful Events in a Rhode Island Coldwater Fish Habitat Using the SWAT Model

    Directory of Open Access Journals (Sweden)

    Britta Chambers

    2017-09-01

    Full Text Available It has become increasingly important to recognize historical water quality trends so that the future impacts of climate change may be better understood. Climate studies have suggested that inland stream temperatures and average streamflow will increase over the next century in New England, thereby putting aquatic species sustained by coldwater habitats at risk. In this study we evaluated two different approaches for modeling historical streamflow and stream temperature in a Rhode Island, USA, watershed with the Soil and Water Assessment Tool (SWAT, using (i original SWAT and (ii SWAT plus a hydroclimatological model component that considers both hydrological inputs and air temperature. Based on daily calibration results with six years of measured streamflow and four years of stream temperature data, we examined occurrences of stressful conditions for brook trout (Salvelinus fontinalis using the hydroclimatological model. SWAT with the hydroclimatological component improved modestly during calibration (NSE of 0.93, R2 of 0.95 compared to the original SWAT (NSE of 0.83, R2 of 0.93. Between 1980–2009, the number of stressful events, a moment in time where high or low flows occur simultaneously with stream temperatures exceeding 21 °C, increased by 55% and average streamflow increased by 60%. This study supports using the hydroclimatological SWAT component and provides an example method for assessing stressful conditions in southern New England’s coldwater habitats.

  2. Inter individual variations of the fish skin microbiota: host genetics basis of mutualism?

    Science.gov (United States)

    Boutin, Sébastien; Sauvage, Christopher; Bernatchez, Louis; Audet, Céline; Derome, Nicolas

    2014-01-01

    The commensal microbiota of fish skin is suspected to provide a protection against opportunist infections. The skin of fish harbors a complex and diverse microbiota that closely interacts with the surrounding water microbial communities. Up to now there is no clear evidence as to whether the host regulates the recruitment of environmental bacteria to build a specific skin microbiota. To address this question, we detected Quantitative Trait Loci (QTL) associated with the abundance of specific skin microbiota bacterial strains in brook charr (Salvelinus fontinalis), combining 16S RNA tagged-amplicon 454 pyrosequencing with genetic linkage analysis. Skin microbiota analysis revealed high inter-individual variation among 86 F2 fish progeny based upon the relative abundance of bacterial operational taxonomic units (OTUs). Out of those OTUs, the pathogenic strain Flavobacterium psychrophilum and the non-pathogenic strain Methylobacterium rhodesianum explained the majority of inter-individual distances. Furthermore, a strong negative correlation was found between Flavobacterium and Methylobacterium, suggesting a mutually competitive relationship. Finally, after considering a total of 266 markers, genetic linkage analysis highlighted three major QTL associated with the abundance of Lysobacter, Rheinheimera and Methylobacterium. All these three genera are known for their beneficial antibacterial activity. Overall, our results provide evidence that host genotype may regulate the abundance of specific genera among their surface microbiota. They also indicate that Lysobacter, Rheinheimera and Methylobacterium are potentially important genera in providing protection against pathogens.

  3. Diagnostic efficacy of molecular assays for the viral haemorrhagic septicaemia virus isolates from the Czech Republic

    Directory of Open Access Journals (Sweden)

    Ľubomír Pojezdal

    2017-01-01

    Full Text Available The diagnostic properties of the one-step real-time reverse-transcription polymerase chain reaction assay for viral haemorrhagic septicaemia virus detection were compared to methods currently in use in the Czech Republic, namely, virus isolation using the cell culture and conventional reverse-transcription polymerase chain reaction followed by the nested polymerase chain reaction. The assays were tested on a panel of 25 archived viral haemorrhagic septicaemia isolates and 8 archived infectious haematopoietic necrosis isolates obtained from monitoring and/or outbreaks of the diseases among farmed salmonids in the Czech Republic. The ability to detect the presence of the virus in the tissues of fish was tested on additional 32 field samples collected from the rainbow trout (Oncorhynchus mykiss, brown trout (Salmo trutta and brook trout (Salvelinus fontinalis. The real-time assay showed the highest analytic sensitivity by detecting the presence of viral nucleic acid in samples with 10-7 dilution, whereas the sensitivity of the conventional polymerase chain reaction peaked at 10-5. Diagnostic specificity of both molecular assays was confirmed by absence of cross-reactivity with the infectious haematopoietic necrosis virus isolates. This, along with consistent results in the detection of the virus in the fish tissues, confirms that the one-step real-time reverse-transcription polymerase chain reaction is currently an optimal stand-alone diagnostic method for the detection of the viral haemorrhagic septicaemia virus.

  4. Influence of eastern hemlock (Tsuga canadensis L.) on fish community structure and function in headwater streams of the Delaware River basin

    Science.gov (United States)

    Ross, R.M.; Bennett, R.M.; Snyder, C.D.; Young, J.A.; Smith, D.R.; Lemarie, D.P.

    2003-01-01

    Hemlock (Tsuga canadensis) forest of the eastern U.S. are in decline due to invasion by the exotic insect hemlock woolly adelgid (Adelges tsugae). Aquatic biodiversity in hemlock ecosystems has not been documented; thus the true impact of the infestation cannot be assessed. We compared ichthyofaunal assemblages and trophic structure of streams draining hemlock and hardwood forests by sampling first- and second-order streams draining 14 paired hemlock and hardwood stands during base flows in July 1997 at the Delaware Water Gap National Recreation Area of Pennsylvania and New Jersey. Over 1400 fish of 15 species and 7 families were collected, but hemlock and hardwood streams individually harbored only one to four species. Brook trout (Salvelinus fontinalis) and brown trout (Salmo trutta) were two to three times as prevalent in hemlock than hardwood streams. Insectivorous fishes occurred in significantly higher proportion in streams of hardwood (0.90) than hemlock (0.46) stands, while piscivores occurred more often in hemlock (0.85) than hardwood (0.54) stands. Functional (trophic) diversity of fishes in hemlock and second-order streams was numerically greater than that of hardwood and first-order streams. Species composition also differed by stream order and terrain type. Biodiversity is threatened at several levels within hemlock ecosystems at risk to the hemlock woolly adelgid in eastern U.S. forests.

  5. Areas contributing recharge to production wells and effects of climate change on the groundwater system in the Chipuxet River and Chickasheen Brook Basins, Rhode Island

    Science.gov (United States)

    Friesz, Paul J.; Stone, Janet R.

    2015-01-01

    The Chipuxet River and Chickasheen Brook Basins in southern Rhode Island are an important water resource for public and domestic supply, irrigation, recreation, and aquatic habitat. The U.S. Geological Survey, in cooperation with the Rhode Island Department of Health, began a study in 2012 as part of an effort to protect the source of water to six large-capacity production wells that supply drinking water and to increase understanding of how climate change might affect the water resources in the basins. Soil-water-balance and groundwater-flow models were developed to delineate the areas contributing recharge to the wells and to quantify the hydrologic response to climate change. Surficial deposits of glacial origin ranging from a few feet to more than 200 feet thick overlie bedrock in the 24.4-square mile study area. These deposits comprise a complex and productive aquifer system.

  6. Monitoring butterflies using counts of puddling males: A case study of the Rajah Brooke's Birdwing (Trogonoptera brookiana albescens.

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    Chooi-Khim Phon

    Full Text Available The Rajah Brooke's Birdwing, Trogonoptera brookiana, is a large, iconic butterfly that is facing heavy commercial exploitation and habitat loss. Males of some subspecies exhibit puddling behavior. A method of conservation monitoring was developed for subspecies albescens in Ulu Geroh, Peninsular Malaysia, where the males consistently puddle in single-species aggregations at stable geothermal springs, reaching well over 300 individuals when the population is at its highest. Digital photography was used to conduct counts of numbers of males puddling. The numbers of birdwings puddling were significantly correlated with counts of birdwings in flight, but were much higher. The numbers puddling during the peak hour were correlated with numbers puddling throughout the day and could be predicted using the numbers puddling at an alternative hour, enabling flexibility in the time of counts. Average counts for three images taken at each puddle at three peak hours between 1400-1600 hours over 2-3 days were used as a monthly population index. The numbers puddling were positively associated with higher relative humidity and brightness during monitoring hours. Monthly counts of birdwings from monitoring of puddles over a period of two years are presented. The minimum effort required for a monitoring program using counts of puddling males is discussed, as well as the potential of using the method to monitor other species of puddling butterflies.

  7. Monitoring butterflies using counts of puddling males: A case study of the Rajah Brooke's Birdwing (Trogonoptera brookiana albescens).

    Science.gov (United States)

    Phon, Chooi-Khim; Kirton, Laurence G; Norma-Rashid, Yusoff

    2017-01-01

    The Rajah Brooke's Birdwing, Trogonoptera brookiana, is a large, iconic butterfly that is facing heavy commercial exploitation and habitat loss. Males of some subspecies exhibit puddling behavior. A method of conservation monitoring was developed for subspecies albescens in Ulu Geroh, Peninsular Malaysia, where the males consistently puddle in single-species aggregations at stable geothermal springs, reaching well over 300 individuals when the population is at its highest. Digital photography was used to conduct counts of numbers of males puddling. The numbers of birdwings puddling were significantly correlated with counts of birdwings in flight, but were much higher. The numbers puddling during the peak hour were correlated with numbers puddling throughout the day and could be predicted using the numbers puddling at an alternative hour, enabling flexibility in the time of counts. Average counts for three images taken at each puddle at three peak hours between 1400-1600 hours over 2-3 days were used as a monthly population index. The numbers puddling were positively associated with higher relative humidity and brightness during monitoring hours. Monthly counts of birdwings from monitoring of puddles over a period of two years are presented. The minimum effort required for a monitoring program using counts of puddling males is discussed, as well as the potential of using the method to monitor other species of puddling butterflies.

  8. Level II scour analysis for Bridge 67 (MTHOTH00120067) on Town Highway 12, crossing Freeman Brook, Mount Holly, Vermont

    Science.gov (United States)

    Wild, Emily C.; Severance, Timothy

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure MTHOTH00120067 on Town Highway 12 crossing Freeman Brook, Mount Holly, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the Green Mountain section of the New England physiographic province in south-central Vermont. The 11.4-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forested. In the study area, Freeman Brook has an incised, sinuous channel with a slope of approximately 0.01 ft/ft, an average channel top width of 51 ft and an average bank height of 6 ft. The channel bed material ranges from sand to boulders with a median grain size (D50) of 55.7 mm (0.183 ft). The geomorphic assessment at the time of the Level I and Level II site visit on October 5, 1995, indicated that the reach was stable. The Town Highway 12 crossing of Freeman Brook is a 34-ft-long, two-lane bridge consisting of a 30-foot prestressed concrete-slab span (Vermont Agency of Transportation, written communication, March 15, 1995). The opening length of the structure parallel to the bridge face is 29.5 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 50 degrees to the opening while the opening-skew-to-roadway is 15 degrees. Along the upstream right wingwall, the right abutment and the downstream right wingwall, a scour hole approximately 1.0 to 2.0 ft deeper than the mean thalweg

  9. Level II scour analysis for Bridge 63 (MTH0TH00120063) on Town Highway 12, crossing Russell Brook, Mount Holly, Vermont

    Science.gov (United States)

    Wild, Emily C.; Severance, Timothy

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure MTHOTH00120063 on Town Highway 12 crossing Russell Brook, Mount Holly, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the Green Mountain section of the New England physiographic province in south-central Vermont. The 3.6-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest upstream and downstream of the bridge. In the study area, Russell Brook has an incised, sinuous channel with a slope of approximately 0.0263 ft/ft, an average channel top width of 29 ft and an average bank height of 3 ft. The channel bed material ranges from cobbles to boulders with a median grain size (D50) of 97.1 mm (0.318 ft). The geomorphic assessment at the time of the Level I and Level II site visit on October 4, 1995, indicated that the reach was stable. The Town Highway 12 crossing of Russell Brook is a 29-ft-long, one-lane bridge consisting of a 26-foot steel-stringer span (Vermont Agency of Transportation, written communication, March 21, 1995). The opening length of the structure parallel to the bridge face is 23.5 ft. The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 40 degrees to the opening while the computed opening-skew-to-roadway is 35 degrees. During the Level I assessment, it was observed that the upstream left wingwall footing was exposed 0.2 ft, in reference to

  10. A Grassroots Community Dialogue on the Ethics of the Care of People with Autism and Their Families: The Stony Brook Guidelines.

    Science.gov (United States)

    Post, Stephen G; Pomeroy, John; Keirns, Carla; Cover, Virginia Isaacs; Dorn, Michael Leverett

    2017-06-01

    The increased recognition and reported prevalence of autism spectrum disorders (ASD) combined with the associated societal and clinical impact call for a broad grassroots community-based dialogue on treatment related ethical and social issues. In these Stony Brook Guidelines, which were developed during a full year of community dialogue (2010-2011) with affected individuals, families, and professionals in the field, we identify and discuss topics of paramount concern to the ASD constituency: treatment goals and happiness, distributive justice, managing the desperate hopes for a cure, sibling responsibilities, intimacy and sex, diagnostic ethics, and research ethics. The members of the dialogue core committee included doctors, ethicists, administrators, social workers, ministers, disability experts, and many family members of individuals with autism who were especially engaged in community activities on behalf of their constituency, including siblings, parents, and grandparents. Our guidelines are not based on "top-down" imposition of professional expertise, but rather on a "bottom-up" grass roots attention to the voices of affected individuals and families speaking from experience. These guidelines can inform clinical practice, but they also are meaningful for the wider social conversation emerging over the treatment of individuals with ASD.

  11. Brooke-Spiegler syndrome presenting multiple concurrent cutaneous and parotid gland neoplasms: cytologic findings on fine-needle sample and description of a novel mutation of the CYLD gene.

    Science.gov (United States)

    Malzone, Maria Gabriella; Campanile, Anna Cipolletta; Losito, Nunzia Simona; Longo, Francesco; Perri, Francesco; Caponigro, Francesco; Schiavone, Concetta; Ionna, Franco; Maiello, Francesco; Martinuzzi, Claudia; Nasti, Sabina; Botti, Gerardo; Fulciniti, Franco

    2015-08-01

    Multiple dermal cylindromas and membranous basal cell adenoma of parotid gland in a 67-year-old woman with Brooke-Spiegler syndrome (BSS) were examined by fine-needle cytology. Histology, immunochemistry, and CYLD germline mutation testing were also performed. Cytomorphology and immunochemistry of the two lesions showed basaloid neoplasms, remarkably similar, composed by proliferating epithelial cells of basal type accompanied by a smaller proportion of myoepithelial cells. CYLD gene showed a novel germline splice acceptor site mutation (c.2042-1G>C) with skipping of the entire exon 15. The occurrence of analogous tumors, dermal cylindromas, and membranous basal cell adenoma of the parotid gland, in the same patient may result from the action of a single gene on ontogenetically similar stem cells. Therefore, patients with BSS should be offered a genetic counselling for an early and correct diagnosis. © 2015 Wiley Periodicals, Inc.

  12. Level II scour analysis for Bridge 25 (ROCHTH00400025) on Town Highway 40, crossing Corporation Brook, Rochester, Vermont

    Science.gov (United States)

    Wild, Emily C.; Weber, Matthew A.

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure ROCHTH00400025 on Town Highway 40 crossing Corporation Brook, Rochester, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, from Vermont Agency of Transportation files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the Green Mountain section of the New England physiographic province in central Vermont. The 4.97-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest on the upstream left and right overbanks, and the downstream left overbank. On the downstream right overbank, the surface cover is predominately brushland. In the study area, Corporation Brook has an incised, sinuous channel with a slope of approximately 0.04 ft/ft, an average channel top width of 37 ft and an average bank height of 6 ft. The channel bed material ranges from gravel to boulders with a median grain size (D50) of 101 mm (0.332 ft). The geomorphic assessment at the time of the Level I site visit on April 12, 1995 and Level I and II site visit on July 8, 1996, indicated that the reach was stable. The Town Highway 40 crossing of Corporation Brook is a 31-ft-long, one-lane bridge consisting of a 26-foot steel stringer span (Vermont Agency of Transportation, written communication, March 22, 1995). The opening length of the structure parallel to the bridge face is 24 ft. The bridge is supported by vertical, concrete abutments. The channel is skewed approximately 15 degrees to the opening while the opening-skew-to-roadway is 15 degrees. A scour hole 1

  13. Level II scour analysis for Bridge 15 (BOLTTH00150015) on Town Highway 15, crossing Joiner Brook, Bolton, Vermont

    Science.gov (United States)

    Burns, Ronda L.; Wild, Emily C.

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure BOLTTH00150015 on Town Highway 15 crossing Joiner Brook, Bolton, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the Green Mountain section of the New England physiographic province in north central Vermont. The 9.6-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is pasture (lawn) downstream of the bridge and on the upstream right bank. The surface cover on the upstream left bank is shrub and brushland. In the study area, Joiner Brook has an incised, straight channel with a slope of approximately 0.01 ft/ft, an average channel top width of 61 ft and an average bank height of 7 ft. The channel bed material ranges from gravel to cobble with a median grain size (D50) of 43.6 mm (0.143 ft). The geomorphic assessment at the time of the Level I and Level II site visit on June 27, 1996, indicated that the reach was stable. The Town Highway 15 crossing of Joiner Brook is a 39-ft-long, two-lane bridge consisting of one 36-foot concrete tee-beam span (Vermont Agency of Transportation, written communication, November 3, 1995). The opening length of the structure parallel to the bridge face is 34.6 ft. The bridge is supported by nearly vertical, concrete abutments with wingwalls. The channel is skewed approximately 10 degrees to the opening while the opening-skew-to-roadway is zero degrees. A scour hole 1.5 ft deeper than the

  14. Level II scour analysis for Bridge 7H (HUNTTH0001007H) on Town Highway 1, crossing Cobb Brook, Huntington, Vermont

    Science.gov (United States)

    Wild, Emily C.

    1997-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure HUNTTH001007H on Town Highway 1 crossing the Cobb Brook, Huntington, Vermont (figures 1–10). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). Results of a Level I scour investigation also are included in Appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in Appendix D.In August 1976, Hurricane Belle caused flooding at this site which resulted in road and bridge damage (figures 7-8). This was approximately a 25-year flood event (U.S. Department of Housing and Urban Development, 1978). The site is in the Green Mountain section of the New England physiographic province in central Vermont. The 4.20-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is forest upstream of the bridge. Downstream of the bridge is brushland and pasture.In the study area, the Cobb Brook has an incised, straight channel with a slope of approximately 0.03 ft/ft, an average channel top width of 43 ft and an average bank height of 6 ft. The channel bed material ranges from sand to boulders with a median grain size (D50) of 65.5 mm (0.215 ft). The geomorphic assessment at the time of the Level I and Level II site visit on June 24, 1996, indicated that the reach was stable. The Town Highway 1 crossing of the Cobb Brook is a 23-ft-long, two-lane bridge consisting of one 20-foot concrete slab span (Vermont Agency of Transportation, written communication, June 21, 1996). The bridge is supported by vertical, concrete abutments with wingwalls. The channel is skewed approximately 15 degrees

  15. Effect of Nutrient Management Planning on Crop Yield, Nitrate Leaching and Sediment Loading in Thomas Brook Watershed

    Science.gov (United States)

    Amon-Armah, Frederick; Yiridoe, Emmanuel K.; Ahmad, Nafees H. M.; Hebb, Dale; Jamieson, Rob; Burton, David; Madani, Ali

    2013-11-01

    Government priorities on provincial Nutrient Management Planning (NMP) programs include improving the program effectiveness for environmental quality protection, and promoting more widespread adoption. Understanding the effect of NMP on both crop yield and key water-quality parameters in agricultural watersheds requires a comprehensive evaluation that takes into consideration important NMP attributes and location-specific farming conditions. This study applied the Soil and Water Assessment Tool (SWAT) to investigate the effects of crop and rotation sequence, tillage type, and nutrient N application rate on crop yield and the associated groundwater leaching and sediment loss. The SWAT model was applied to the Thomas Brook Watershed, located in the most intensively managed agricultural region of Nova Scotia, Canada. Cropping systems evaluated included seven fertilizer application rates and two tillage systems (i.e., conventional tillage and no-till). The analysis reflected cropping systems commonly managed by farmers in the Annapolis Valley region, including grain corn-based and potato-based cropping systems, and a vegetable-horticulture system. ANOVA models were developed and used to assess the effects of crop management choices on crop yield and two water-quality parameters (i.e., leaching and sediment loading). Results suggest that existing recommended N-fertilizer rate can be reduced by 10-25 %, for grain crop production, to significantly lower leaching ( P > 0.05) while optimizing the crop yield. The analysis identified the nutrient N rates in combination with specific crops and rotation systems that can be used to manage leaching while balancing impacts on crop yields within the watershed.

  16. Contributions of separate reactions to the acid-base buffering of soils in brook floodplains (Central Forest State Reserve)

    Science.gov (United States)

    Sokolova, T. A.; Tolpeshta, I. I.; Rusakova, E. S.

    2016-04-01

    The acid-base buffering of gleyic gray-humus soils developed in brook floodplains and undisturbed southern-taiga landscapes has been characterized by the continuous potentiometric titration of soil water suspensions. During the interaction with an acid, the major amount of protons (>80%) is consumed for the displacement of exchangeable bases and the dissolution of Ca oxalates. In the O and AY horizons, Mn compounds make the major contribution (2-15%) to the acid buffering. The buffer reactions with the participation of Al compounds make up from 0.5 to 1-2% of the total buffering capacity, and the protonation of the surface OH groups of kaolinite consumes 2-3% of the total buffering capacity. The deprotonation of OH groups on the surface of Fe hydroxides (9-43%), the deprotonation of OH groups on the surface of illite crystals (3-19%), and the dissolution of unidentified aluminosilicates (9-14%) are the most significant buffer reactions whose contributions have been quantified during the interaction with a base. The contribution of the deprotonation of OH groups on the surface of kaolinite particles is lower (1-5%) because of the small specific surface area of this mineral, and that of the dissolution of Fe compounds is insignificant. In the AY horizon, the acid and base buffering of soil in the rhizosphere is higher than beyond the rhizosphere because of the higher contents of organic matter and nonsilicate Fe and Al compounds.

  17. Assessing the impacts of river regulation on native bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) habitats in the upper Flathead River, Montana, USA

    Science.gov (United States)

    Muhlfeld, Clint C.; Jones, Leslie A.; Kotter, D.; Miller, William J.; Geise, Doran; Tohtz, Joel; Marotz, Brian

    2012-01-01

    Hungry Horse Dam on the South Fork Flathead River, Montana, USA, has modified the natural flow regimen for power generation, flood risk management and flow augmentation for anadromous fish recovery in the Columbia River. Concern over the detrimental effects of dam operations on native resident fishes prompted research to quantify the impacts of alternative flow management strategies on threatened bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) habitats. Seasonal and life‐stage specific habitat suitability criteria were combined with a two‐dimensional hydrodynamic habitat model to assess discharge effects on usable habitats. Telemetry data used to construct seasonal habitat suitability curves revealed that subadult (fish that emigrated from natal streams to the river system) bull trout move to shallow, low‐velocity shoreline areas at night, which are most sensitive to flow fluctuations. Habitat time series analyses comparing the natural flow regimen (predam, 1929–1952) with five postdam flow management strategies (1953–2008) show that the natural flow conditions optimize the critical bull trout habitats and that the current strategy best resembles the natural flow conditions of all postdam periods. Late summer flow augmentation for anadromous fish recovery, however, produces higher discharges than predam conditions, which reduces the availability of usable habitat during this critical growing season. Our results suggest that past flow management policies that created sporadic streamflow fluctuations were likely detrimental to resident salmonids and that natural flow management strategies will likely improve the chances of protecting key ecosystem processes and help to maintain and restore threatened bull trout and westslope cutthroat trout populations in the upper Columbia River Basin.

  18. Bull trout (Salvelinus confluentus) movement in relation to water temperature, season, and habitat features in Arrowrock Reservoir, Idaho, 2012

    Science.gov (United States)

    Maret, Terry R.; Schultz, Justin E.

    2013-01-01

    Acoustic telemetry was used to determine spring to summer (April–August) movement and habitat use of bull trout (Salvelinus confluentus) in Arrowrock Reservoir (hereafter “Arrowrock”), a highly regulated reservoir in the Boise River Basin of southwestern Idaho. Water management practices annually use about 86 percent of the reservoir water volume to satisfy downstream water demands. These practices might be limiting bull trout habitat and movement patterns. Bull trout are among the more thermally sensitive coldwater species in North America, and the species is listed as threatened throughout the contiguous United States under the Endangered Species Act. Biweekly water-temperature and dissolved-oxygen profiles were collected by the Bureau of Reclamation at three locations in Arrowrock to characterize habitat conditions for bull trout. Continuous streamflow and water temperature also were measured immediately upstream of the reservoir on the Middle and South Fork Boise Rivers, which influence habitat conditions in the riverine zones of the reservoir. In spring 2012, 18 bull trout ranging in total length from 306 to 630 millimeters were fitted with acoustic transmitters equipped with temperature and depth sensors. Mobile boat tracking and fixed receivers were used to detect released fish. Fish were tagged from March 28 to April 20 and were tracked through most of August. Most bull trout movements were detected in the Middle Fork Boise River arm of the reservoir. Fifteen individual fish were detected at least once after release. Water surface temperature at each fish detection location ranged from 6.0 to 16.2 degrees Celsius (°C) (mean=10.1°C), whereas bull trout body temperatures were colder, ranging from 4.4 to 11.6°C (mean=7.3°C). Bull trout were detected over deep-water habitat, ranging from 8.0 to 42.6 meters (m) (mean=18.1 m). Actual fish depths were shallower than total water depth, ranging from 0.0 to 24.5 m (mean=6.7 m). The last bull trout was

  19. Level II scour analysis for Bridge 34 (WWINTH00370034) on Town Highway 37, crossing Mill Brook, West Windsor, Vermont

    Science.gov (United States)

    Boehmler, Erick M.; Wild, Emily C.

    1998-01-01

    This report provides the results of a detailed Level II analysis of scour potential at structure WWINTH00370034 on Town Highway 37 crossing Mill Brook, West Windsor, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (FHWA, 1993). Results of a Level I scour investigation also are included in appendix E of this report. A Level I investigation provides a qualitative geomorphic characterization of the study site. Information on the bridge, gleaned from Vermont Agency of Transportation (VTAOT) files, was compiled prior to conducting Level I and Level II analyses and is found in appendix D. The site is in the New England Upland section of the New England physiographic province in east-central Vermont. The 16.6-mi2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the surface cover is pasture except for the upstream left bank where there is mostly shrubs and brush. In the study area, Mill Brook has a sinuous channel with a slope of approximately 0.003 ft/ ft, an average channel top width of 52 ft and an average bank height of 5 ft. The channel bed material ranges from sand to cobbles with a median grain size (D50) of 43.4 mm (0.142 ft). The geomorphic assessment at the time of the Level I and Level II site visit on June 5, 1996, indicated that the reach was laterally unstable. Point bars were observed upstream and downstream of this site. Furthermore, slip failure of the bank material was noted downstream at a cut-bank on the left side of the channel across from a point bar. The Town Highway 37 crossing of Mill Brook is a 37-ft-long, one-lane covered bridge consisting of one 32-foot wood thru-truss span (Vermont Agency of Transportation, written communication, March 23, 1995). The opening length of the structure parallel to the bridge face is 29.6 ft. The bridge is supported by vertical, laid-up stone abutment walls with

  20. Brooks, T., y Nussbaum, M. C. (eds.. (2015. Rawls's Political Liberalism. Nueva York, Estados Unidos de América: Columbia University. 206 pp.

    Directory of Open Access Journals (Sweden)

    Mario Josue Cunningham-Matamoros

    2017-06-01

    Full Text Available Poco más de dos décadas después de la publicación de Political Liberalism (1993 del filósofo estadounidense John Rawls, Thom Brooks y Martha Nussbaum se dieron la tarea de editar una compilación de seis ensayos que muestran la actualidad de este libro. Los escritos que participan en esta recopilación se aproximan al texto rawlsiano de manera variopinta, tanto a nivel disciplinar como en lo referido a la finalidad con la cual lo abordan. A grandes rasgos, estos se dividen en tres grupos: el primer grupo, cuya pretensión es realizar una revisión crítica de la obra o de conceptos medulares de esta (Frank I. Michelman, Martha Nussbaum y Onora O'Neill; el segundo conjunto, donde se realiza una labor exegética capaz de responder a múltiples críticas que ha enfrentado el texto desde su publicación (Jeremy Waldron y Paul Weithman y, finalmente, un artículo ―el tercer grupo― que muestra la consistencia práctica en el modo en que Rawls concibió el derecho constitucional estadounidense.

  1. Predicting recolonization patterns and interactions between potamodromous and anadromous salmonids in response to dam removal in the Elwha River, Washington State, USA

    Science.gov (United States)

    Brenkman, S.J.; Pess, G.R.; Torgersen, C.E.; Kloehn, K.K.; Duda, J.J.; Corbett, S.C.

    2008-01-01

    The restoration of salmonids in the Elwha River following dam removal will cause interactions between anadromous and potamodromous forms as recolonization occurs in upstream and downstream directions. Anadromous salmonids are expected to recolonize historic habitats, and rainbow trout (Oncorhynchus mykiss) and bull trout (Salvelinus confluentus) isolated above the dams for 90 years are expected to reestablish anadromy. We summarized the distribution and abundance of potamodromous salmonids, determined locations of spawning areas, and mapped natural barriers to fish migration at the watershed scale based on data collected from 1993 to 2006. Rainbow trout were far more abundant than bull trout throughout the watershed and both species were distributed up to river km 71. Spawning locations for bull trout and rainbow trout occurred in areas where we anticipate returning anadromous fish to spawn. Nonnative brook trout were confined to areas between and below the dams, and seasonal velocity barriers are expected to prevent their upstream movements. We hypothesize that the extent of interaction between potamodromous and anadromous salmonids will vary spatially due to natural barriers that will limit upstream-directed recolonization for some species of salmonids. Consequently, most competitive interactions will occur in the main stem and floodplain downstream of river km 25 and in larger tributaries. Understanding future responses of Pacific salmonids after dam removal in the Elwha River depends upon an understanding of existing conditions of the salmonid community upstream of the dams prior to dam removal.

  2. Inter individual variations of the fish skin microbiota: host genetics basis of mutualism?

    Directory of Open Access Journals (Sweden)

    Sébastien Boutin

    Full Text Available The commensal microbiota of fish skin is suspected to provide a protection against opportunist infections. The skin of fish harbors a complex and diverse microbiota that closely interacts with the surrounding water microbial communities. Up to now there is no clear evidence as to whether the host regulates the recruitment of environmental bacteria to build a specific skin microbiota. To address this question, we detected Quantitative Trait Loci (QTL associated with the abundance of specific skin microbiota bacterial strains in brook charr (Salvelinus fontinalis, combining 16S RNA tagged-amplicon 454 pyrosequencing with genetic linkage analysis. Skin microbiota analysis revealed high inter-individual variation among 86 F2 fish progeny based upon the relative abundance of bacterial operational taxonomic units (OTUs. Out of those OTUs, the pathogenic strain Flavobacterium psychrophilum and the non-pathogenic strain Methylobacterium rhodesianum explained the majority of inter-individual distances. Furthermore, a strong negative correlation was found between Flavobacterium and Methylobacterium, suggesting a mutually competitive relationship. Finally, after considering a total of 266 markers, genetic linkage analysis highlighted three major QTL associated with the abundance of Lysobacter, Rheinheimera and Methylobacterium. All these three genera are known for their beneficial antibacterial activity. Overall, our results provide evidence that host genotype may regulate the abundance of specific genera among their surface microbiota. They also indicate that Lysobacter, Rheinheimera and Methylobacterium are potentially important genera in providing protection against pathogens.

  3. Agonistic behavior among three stocked trout species in a novel reservoir fish community

    Science.gov (United States)

    Budy, Phaedra; Hafen, Konrad

    2015-01-01

    The popularity of reservoirs to support sport fisheries has led to the stocking of species that did not co-evolve, creating novel reservoir fish communities. In Utah, the Bear Lake strain of Bonneville Cutthroat Trout Oncorhynchus clarkii utah and tiger trout (female Brown Trout Salmo trutta × male Brook Trout Salvelinus fontinalis) are being more frequently added to a traditional stocking regimen consisting primarily of Rainbow TroutO. mykiss. Interactions between these three predatory species are not well understood, and studies evaluating community interactions have raised concern for an overall decrease of trout condition. To evaluate the potential for negative interactions among these species, we tested aggression in laboratory aquaria using three-species and pairwise combinations at three densities. Treatments were replicated before and after feeding. During the three-species trials Rainbow Trout initiated 24.8 times more aggressive interactions than Cutthroat Trout and 10.2 times more aggressive interactions than tiger trout, and tiger trout exhibited slightly (1.9 times) more aggressive initiations than Cutthroat Trout. There was no significant difference in behavior before versus after feeding for any species, and no indication of increased aggression at higher densities. Although Rainbow Trout in aquaria may benefit from their bold, aggressive behavior, given observations of decreased relative survival in the field, these benefits may be outweighed in reservoirs, possibly through unnecessary energy expenditure and exposure to predators.

  4. The food of the lake trout (Cristivomer namaycush namaycush) and of the lawyer (Lota maculosa) of Lake Michigan

    Science.gov (United States)

    Van Oosten, John; Deason, Hilary J.

    1938-01-01

    This paper reports on a qualitative and quantitative analysis of the contents of 4,979 lake trout stomachs (593 examined in 1930 and 1,253 collected in 1931 from southern Lake Michigan, 1,446 from northern Lake Michigan and 1,687 from Green Bay in 1932), and of a total of 1,528 lawyer stomachs (172 examined in 1930 and 734 collected in 1931 from southern Lake Michigan, 612 from northern Lake Michigan and 10 from Green Bay in 1932). The food of the trout consisted of 98 per cent by volume of fish of which Cottidae and Coregonidae were the principal constituents. Cottidae were dominant in southern Lake Michigan (72 per cent by volume), Coregonidae in northern Lake Michigan (51 per cent) but the lake shiner, Notropis atherinides, was most important in Green Bay in the spring of the year (64 per cent). The lawyer food consisted of 74 per cent by volume of fish and 26 per cent invertebrates. Dominant items were Cottidae (76 per cent by volume) in southern Lake Michigan, Coregonidae (51 per cent) and Pontoporeia (37 per cent) in northern Lake Michigan, and Percopsis (34 per cent) and Mysis (26 per cent) in Green Bay. Data are also presented on the frequency of occurrence (number of stomachs) of the food items and its variation with the sizes of the trout and lawyers, depths of water, seasons, and localities; on the number of individual fish of each species destroyed by the trout and lawyers; and on the calculated volume of the food fishes preceding digestion. The lake trout and lawyer are competitors for the same food, are both predators of the commercially important Coregonidae, and the lawyer through its consumption of invertebrates is a food competitor of the Coregonidae.

  5. Metabolic interactions between low doses of benzo[a]pyrene and tributyltin in arctic charr (salvelinus alpinus): a long-term in vivo study

    International Nuclear Information System (INIS)

    Padros, Jaime; Pelletier, Emilien; Ribeiro, Ciro Oliveira

    2003-01-01

    We have previously reported that short-term, single exposure to a high dose of tributyltin (TBT), a widely used antifouling biocide, inhibited both the in vivo metabolism and metabolic activation of the carcinogenic polycyclic aromatic hydrocarbon benzo[a]pyrene (BaP) in fish; (BaP), in turn, stimulated TBT metabolism. Here, we provide further mechanistic evidence of mutual metabolic interactions between BaP and TBT in response to long-term, repeated exposures to low doses. Juvenile Arctic charr (Salvelinus alpinus) received 10 separate ip injections (a single injection every 6 days) of BaP (3 mg/kg), TBT (0.3 mg/kg), or both in combination; control fish received corn oil vehicle only. Two days after the 2nd (Day 8), 6 th (Day 32), and 10th dose (Day 56), blood, bile, and liver samples were collected and analyzed for a suite of biomarkers. HPLC/fluorescence analysis indicated that TBT cotreatment inhibited the formation of (+)-anti-BaP diol-epoxide adducts with plasma albumin (53%, Day 8), hepatic DNA (27%, Day 32), or both albumin and globin (50 and 58%, Day 56) compared to BaP alone. This antagonistic interaction was associated with a time-dependent modulation (inhibition at Day 8, enhancement at Day 32) of both cytochrome P450 (P450)1A-mediated ethoxyresorufin O-deethylase (EROD) activity and biliary BaP metabolite formation. TBT cotreatment also inhibited (39%) the BaP-mediated induction of hepatic glutathione S-transferase (GST) activity observed at Day 8. Treatment with TBT alone increased EROD activity (60%) at Day 32, but decreased both GST activity (70 and 37%) and glutathione content (24% and 16%) at Days 32 and 56, respectively. GC/MS analysis revealed that, at Day 56, BaP cotreatment increased (200%) the levels of biliary butyltin compounds, including mono- and dibutyltin metabolites. This potentiative interaction was associated with a protective effect of BaP cotreatment against the TBT-mediated decreases in GST activity and glutathione content. The

  6. Evidence that lake trout served as a buffer against sea lamprey predation on burbot in Lake Erie

    Science.gov (United States)

    Stapanian, M.A.; Madenjian, C.P.

    2007-01-01

    The population of burbot Lota lota in Lake Erie recovered during 1986–2003, mainly because of the control of sea lamprey Petromyzon marinus, which began in 1986. Burbot populations continued to grow during 1996–1998, when sea lamprey control was substantially reduced. We calculated mortality parameters for burbot in Lake Erie by estimating age at capture for 2,793 burbot caught in annual gill-net surveys of eastern Lake Erie from 1994 to 2003. Based on catch-curve analysis, annual mortality in Lake Erie during 1994–2003 was estimated as 33%. Annual mortality of the 1992 year-class of burbot was estimated as 30%. The mortality of burbot during the years of reduced sea lamprey control was not different from that during the 3 years preceding reduced control and was significantly lower than that during the entire portion of the time series in which full sea lamprey control was conducted. These results suggest that the reduction in sea lamprey control did not lead to increased burbot mortality. The catch per gill-net lift of large burbot (total length > 600 mm), the size preferred by sea lampreys, was lower than that of adult lake trout Salvelinus namaycush (age 5 and older; total length > 700 mm) before lampricide application was reduced. Although adult lake trout populations declined, the abundance of large burbot did not change during the period of reduced lampricide application. These results support a hypothesis that a healthy population of adult lake trout can serve as a buffer species, acting to reduce predation of burbot by sea lampreys when sea lamprey populations increase. Burbot attained sexual maturity at a relatively early age (3 or 4 years) and a total length (approximately 500 mm) that was smaller than the preferred prey size for sea lampreys. These characteristics and the buffering effect of the lake trout population enabled growth of the burbot population during the brief period when lamprey control was reduced.

  7. Mercury Dynamics in Aquatic Food Webs of the Finger Lakes, New York

    Science.gov (United States)

    Cleckner, L.; Razavi, N. R.; Halfman, J. D.; Cushman, S. F.; Foust, J.; Gilman, B.

    2016-12-01

    Mercury (Hg) contamination of fish is a global concern due to the deleterious health effects in humans and wildlife associated with ingesting fish with elevated concentrations. A key to understanding elevated fish Hg concentrations is to examine methyl Hg dynamics at the base of food webs, including algae and zooplankton. Predicting determinants of methyl Hg concentrations in lower trophic level biota remains an active area of research. This study was conducted to assess Hg concentrations in biota of the Finger Lakes (New York, USA), a region where fisheries are an important economic driver, but where no comprehensive assessment of food web Hg dynamics has been completed to date. Sources of Hg in the region include atmospheric pollution from an active coal-fired power plant. The objectives of this study were to: 1) determine if fish Hg concentrations were of concern, 2) assess differences in Hg accumulation among lakes and determine predictors of fish Hg concentrations, and 3) evaluate the predictive power of monthly zooplankton methyl Hg concentrations on fish Hg concentrations. From May - October 2015, suspended particulate matter, zooplankton, and benthos were sampled monthly in five of the Finger Lakes (Honeoye, Canandaigua, Seneca, Cayuga, and Owasco Lakes). Fish were sampled once over the same study period and species were targeted from all trophic levels. Results for top predatory fish including Lake Trout (Salvelinus namaycush), Largemouth Bass (Micropterus salmoides), and Walleye (Sander vitreus) showed significant differences among lakes, and elevated concentrations are above US Environmental Protection Agency's screening value (300 ng/g wet weight). No clear pattern in Hg levels among lakes was evident in lower trophic level fishes such as Yellow Perch (Perca flavescens) and Golden Shiner (Notemigonus crysoleucas), but concentrations were low. Benthivorous Brown Bullhead (Ameiurus nebulosus) exhibited significant differences in Hg among lakes with

  8. Contrasting past and current numbers of bears visiting Yellowstone cutthroat trout streams

    Science.gov (United States)

    Haroldson, Mark A.; Schwartz, Charles C.; Teisberg, Justin E.; Gunther, Kerry A.; Fortin, Jennifer K.; Robbins, Charles T.

    2014-01-01

    Spawning cutthroat trout (Oncorhynchus clarkii bouvieri) were historically abundant within tributary streams of Yellowstone Lake within Yellowstone National Park and were a highly digestible source of energy and protein for Yellowstone’s grizzly bears (Ursus arctos) and black bears (U. americanus). The cutthroat trout population has subsequently declined since the introduction of non-native lake trout (Salvelinus namaycush), and in response to effects of drought and whirling disease (Myxobolus cerebralis). The trout population, duration of spawning runs, and indices of bear use of spawning streams had declined in some regions of the lake by 1997–2000. We initiated a 3-year study in 2007 to assess whether numbers of spawning fish, black bears, and grizzly bears within and alongside stream corridors had changed since 1997– 2000. We estimated numbers of grizzly bears and black bears by first compiling encounter histories of individual bears visiting 48 hair-snag sites along 35 historically fished streams.We analyzed DNA encounter histories with Pradel-recruitment and Jolly-Seber (POPAN) capture-mark-recapture models. When compared to 1997–2000, the current number of spawning cutthroat trout per stream and the number of streams with cutthroat trout has decreased. We estimated that 48 (95% CI¼42–56) male and 23 (95% CI¼21–27) female grizzly bears visited the historically fished tributary streams during our study. In any 1- year, 46 to 59 independent grizzly bears (8–10% of estimated Greater Yellowstone Ecosystem population) visited these streams. When compared with estimates from the 1997 to 2000 study and adjusted for equal effort, the number of grizzly bears using the stream corridors decreased by 63%. Additionally, the number of black bears decreased between 64% and 84%. We also document an increased proportion of bears of both species visiting front-country (i.e., near human development) streams. With the recovery of cutthroat trout, we suggest bears

  9. La construcción del personaje lésbico en los relatos cubanos de Sonia Rivera-Valdés y Jacqueline Herranz-Brooks: de la “abyección” a la subversión

    Directory of Open Access Journals (Sweden)

    Sophie Cabaloue

    2013-06-01

    Full Text Available En este ensayo se analizan las obras de dos autoras cubanas de la diáspora, Sonia Rivera-Valdés y Jacqueline Herranz-Brooks, enfocando especialmente la temática lesbiana de estas narraciones, con especial atención a la forma como nos revelan la situación de las lesbianas en Cuba, sobre todo en el “Periodo especial”, a la vez que se convierte en un modo de subvertir la heterosexualidad obligatoria. De este modo se indaga sobre cómo en sus relatos se construye el sujeto “abyecto” (el personaje lésbico en oposición al sistema heteronormativo y cómo este sujeto pasa de la “abyección” a la subversión, al desafiar la heterosexualidad obligatoria.

  10. Factors affecting reservoir and stream-water quality in the Cambridge, Massachusetts, drinking-water source area and implications for source-water protection

    Science.gov (United States)

    Waldron, Marcus C.; Bent, Gardner C.

    2001-01-01

    This report presents the results of a study conducted by the U.S. Geological Survey, in cooperation with the city of Cambridge, Massachusetts, Water Department, to assess reservoir and tributary-stream quality in the Cambridge drinking-water source area, and to use the information gained to help guide the design of a comprehensive water-quality monitoring program for the source area. Assessments of the quality and trophic state of the three primary storage reservoirs, Hobbs Brook Reservoir, Stony Brook Reservoir, and Fresh Pond, were conducted (September 1997-November 1998) to provide baseline information on the state of these resources and to determine the vulnerability of the reservoirs to increased loads of nutrients and other contaminants. The effects of land use, land cover, and other drainage-basin characteristics on sources, transport, and fate of fecal-indicator bacteria, highway deicing chemicals, nutrients, selected metals, and naturally occurring organic compounds in 11 subbasins that contribute water to the reservoirs also was investigated, and the data used to select sampling stations for incorporation into a water-quality monitoring network for the source area. All three reservoirs exhibited thermal and chemical stratification, despite artificial mixing by air hoses in Stony Brook Reservoir and Fresh Pond. The stratification produced anoxic or hypoxic conditions in the deepest parts of the reservoirs and these conditions resulted in the release of ammonia nitrogen orthophosphate phosphorus, and dissolved iron and manganese from the reservoir bed sediments. Concentrations of sodium and chloride in the reservoirs usually were higher than the amounts recommended by the U.S. Environmental Protection agency for drinking-water sources (20 milligrams per liter for sodium and 250 milligrams per liter for chloride). Maximum measured sodium concentrations were highest in Hobbs Brook Reservoir (113 milligrams per liter), intermediate in Stony Brook Reservoir (62

  11. Simulation of temporal and spatial soot evolution in an automotive diesel engine using the Moss–Brookes soot model

    International Nuclear Information System (INIS)

    Pang, Kar Mun; Ng, Hoon Kiat; Gan, Suyin

    2012-01-01

    Highlights: ► Numerical models were validated against experimental data of two diesel engines. ► Soot model constant values were calibrated to predict in-cylinder soot processes. ► Effects of split-main injection parameters on soot distributions were determined. ► Soot cloud was distributed towards cylinder wall when using large dwell period. ► Greater soot deposition expected with large dwell period and retarded injection. - Abstract: In this reported work, computational study on the formation processes of soot particles from diesel combustion is conducted using an approach where Computational Fluid Dynamics (CFD) is coupled with a chemical kinetic model. A multi-step soot model which accounts for inception, surface growth, coagulation and oxidation was applied. Model constant values in the Moss–Brookes soot formation and Fenimore–Jones soot oxidation models were calibrated, and were validated against in-cylinder soot evolution and exhaust soot density of both heavy- and light-duty diesel engines, respectively. Effects of various injection parameters such as start of injection (SOI) timing, split-main ratio and dwell period of the split-main injection strategy on in-cylinder temporal/spatial soot evolution in a light-duty diesel engine were subsequently investigated. The spatial soot distributions at each crank angle degree after start of injection were found to be insensitive to the change of values in SOI and split-main ratio when close-coupled injection was implemented. Soot cloud was also observed to be distributed towards the cylinder wall when a large separation of 20° was used, even with an advanced SOI timing of −6° after top dead centre (ATDC). The use of large separation is hence not desired for this combustion system as it potentially leads to soot deposition on surface oil film and greater tailpipe soot emissions.

  12. Functional morphology and biomechanics of the tongue-bite apparatus in salmonid and osteoglossomorph fishes

    Science.gov (United States)

    Camp, Ariel L; Konow, Nicolai; Sanford, Christopher P J

    2009-01-01

    The tongue-bite apparatus and its associated musculoskeletal elements of the pectoral girdle and neurocranium form the structural basis of raking, a unique prey-processing behaviour in salmonid and osteoglossomorph fishes. Using a quantitative approach, the functional osteology and myology of this system were compared between representatives of each lineage, i.e. the salmonid Salvelinus fontinalis (N =10) and the osteoglossomorph Chitala ornata(N = 8). Divergence was found in the morphology of the novel cleithrobranchial ligament, which potentially relates to kinematic differences between the raking lineage representatives. Salvelinus had greater anatomical cross-sectional areas of the epaxial, hypaxial and protractor hyoideus muscles, whereas Chitala had greater sternohyoideus and adductor mandibulae mass. Two osteology-based biomechanical models (a third-order lever for neurocranial elevation and a modified four-bar linkage for hyoid retraction) showed divergent force/velocity priorities in the study taxa. Salvelinus maximizes both force (via powerful cranial muscles) and velocity (through mechanical amplification) during raking. In contrast, Chitala has relatively low muscle force but more efficient force transmission through both mechanisms compared with Salvelinus. It remains unclear if and how behavioural modulation and specializations in the post-cranial anatomy may affect the force/velocity trade-offs in Chitala. Further studies of tongue-bite apparatus morphology and biomechanics in a broader species range may help to clarify the role that osteology and myology play in the evolution of behavioural diversity. PMID:19438765

  13. A 12-month descriptive analysis of emergency intubations at Brooke Army Medical Center: a National Emergency Airway Registry study.

    Science.gov (United States)

    April, Michael D; Schauer, Steven G; Brown Rd, Calvin A; Ng, Patrick C; Fernandez, Jessie; Fantegrossi, Andrea E; Maddry, Joseph K; Summers, Shane; Sessions, Daniel J; Barnwell, Robert M; Antonacci, Mark

    2017-01-01

    Emergency airway management is a critical skill for military healthcare providers. Our goal was to describe the Emergency Department (ED) intubations at Brooke Army Medical Center (BAMC) over a 12-month period. Physicians performing endotracheal intubations in the BAMC ED complete data collection forms for each intubation event as part of the National Emergency Airway Registry, including patient demographics, intubation techniques, success and failure rates, adverse events, and patient disposition. We cross-referenced these forms against the numbers of intubation events reported in the ED nursing daily reports to ensure capture of all intubations. Providers completed forms for every intubation within 6 weeks of the procedure. We analyzed data from March 28, 2016, to March 27, 2017. During the study period, providers performed 259 intubations in the BAMC ED. Reasons for intubation were related to trauma for 184 patients (71.0%) and medical conditions for 75 patients (29.0%). Overall, first-attempt success was 83.0%. Emergency medicine residents performed a majority of first attempts (95.0%). Most common devices chosen on first attempt were a video laryngoscope for 143 patients (55.2%) and a direct laryngoscope for 115 patients (44.4%). One patient underwent cricothyrotomy. The 2 most common induction agents were ketamine (59.8%; 95% CI, 55.2%-67.4%) and etomidate (19.3%; 95% CI, 14.7%-24.7%). The most common neuromuscular blocking agents were rocuronium (62.9%; 95% CI, 56.7%-68.8%) and succinylcholine (18.9%; 95% CI, 14.3%-24.2%). In the BAMC ED, emergency intubation most commonly occurred for trauma indications using video laryngoscopy with a high first-pass success.

  14. SoundScapes: non-formal learning potentials from interactive VEs

    DEFF Research Database (Denmark)

    Brooks, Tony; Petersson, Eva

    2007-01-01

    Non-formal learning is evident from an inhabited information space that is created from non-invasive multi-dimensional sensor technologies that source human gesture. Libraries of intuitive interfaces empower natural interaction where the gesture is mapped to the multisensory content. Large screen...... and international bodies have consistently recognized SoundScapes which, as a research body of work, is directly responsible for numerous patents. Please note that my full name is Anthony Lewis Brooks. I publish with Anthony Brooks: A. L. Brooks; Tony Brooks.  ...

  15. A VRML-Based Data Portal: Hydrology of the Hubbard Brook Experimental Forest and Mirror Lake Sub-Basin

    Science.gov (United States)

    Becker, M. W.; Bursik, M. I.; Schuetz, J. W.

    2001-05-01

    The Hubbard Brook Experimental Forest (HBEF) of Central New Hampshire has been a focal point for collaborative hydrologic research for over 40 years. A tremendous amount of data from this area is available through the internet and other sources, but is not organized in a manner that facilitates teaching of hydrologic concepts. The Mirror Lake Watershed Interactive Teaching Database is making hydrologic data from the HBEF and associated interactive problem sets available to upper-level and post-graduate university students through a web-based resource. Hydrologic data are offered via a three-dimensional VRML (Virtual Reality Modeling Language) interface, that facilitates viewing and retrieval in a spatially meaningful manner. Available data are mapped onto a topographic base, and hot spots representing data collection points (e.g. weirs) lead to time-series displays (e.g. hydrographs) that provide a temporal link to the spatially organized data. Associated instructional exercises are designed to increase understanding of both hydrologic data and hydrologic methods. A pedagogical module concerning numerical ground-water modeling will be presented as an example. Numerical modeling of ground-water flow involves choosing the combination of hydrogeologic parameters (e.g. hydraulic conductivity, recharge) that cause model-predicted heads to best match measured heads in the aquifer. Choosing the right combination of parameters requires careful judgment based upon knowledge of the hydrogeologic system and the physics of ground-water flow. Unfortunately, students often get caught up in the technical aspects and lose sight of the fundamentals when working with real ground-water software. This module provides exercises in which a student chooses model parameters and immediately sees the predicted results as a 3-D VRML object. VRML objects are based upon actual Modflow model results corresponding to the range of model input parameters available to the student. This way, the

  16. 75 FR 50955 - Proposed Flood Elevation Determinations

    Science.gov (United States)

    2010-08-18

    ... community may at any time enact stricter requirements of its own or pursuant to policies established by... terminus of Halcyon Drive. Coffin Brook Just upstream of the None +133 Town of Berwick. confluence with... Brook Tributary 1 Just upstream of the None +141 Town of Berwick. confluence with Coffin Brook. Just...

  17. GPU-Accelerated Stony-Brook University 5-class Microphysics Scheme in WRF

    Science.gov (United States)

    Mielikainen, J.; Huang, B.; Huang, A.

    2011-12-01

    The Weather Research and Forecasting (WRF) model is a next-generation mesoscale numerical weather prediction system. Microphysics plays an important role in weather and climate prediction. Several bulk water microphysics schemes are available within the WRF, with different numbers of simulated hydrometeor classes and methods for estimating their size fall speeds, distributions and densities. Stony-Brook University scheme (SBU-YLIN) is a 5-class scheme with riming intensity predicted to account for mixed-phase processes. In the past few years, co-processing on Graphics Processing Units (GPUs) has been a disruptive technology in High Performance Computing (HPC). GPUs use the ever increasing transistor count for adding more processor cores. Therefore, GPUs are well suited for massively data parallel processing with high floating point arithmetic intensity. Thus, it is imperative to update legacy scientific applications to take advantage of this unprecedented increase in computing power. CUDA is an extension to the C programming language offering programming GPU's directly. It is designed so that its constructs allow for natural expression of data-level parallelism. A CUDA program is organized into two parts: a serial program running on the CPU and a CUDA kernel running on the GPU. The CUDA code consists of three computational phases: transmission of data into the global memory of the GPU, execution of the CUDA kernel, and transmission of results from the GPU into the memory of CPU. CUDA takes a bottom-up point of view of parallelism is which thread is an atomic unit of parallelism. Individual threads are part of groups called warps, within which every thread executes exactly the same sequence of instructions. To test SBU-YLIN, we used a CONtinental United States (CONUS) benchmark data set for 12 km resolution domain for October 24, 2001. A WRF domain is a geographic region of interest discretized into a 2-dimensional grid parallel to the ground. Each grid point has

  18. Low reproductive isolation and highly variable levels of gene flow reveal limited progress towards speciation between European river and brook lampreys.

    Science.gov (United States)

    Rougemont, Q; Gaigher, A; Lasne, E; Côte, J; Coke, M; Besnard, A-L; Launey, S; Evanno, G

    2015-12-01

    Ecologically based divergent selection is a factor that could drive reproductive isolation even in the presence of gene flow. Population pairs arrayed along a continuum of divergence provide a good opportunity to address this issue. Here, we used a combination of mating trials, experimental crosses and population genetic analyses to investigate the evolution of reproductive isolation between two closely related species of lampreys with distinct life histories. We used microsatellite markers to genotype over 1000 individuals of the migratory parasitic river lamprey (Lampetra fluviatilis) and freshwater-resident nonparasitic brook lamprey (Lampetra planeri) distributed in 10 sympatric and parapatric population pairs in France. Mating trials, parentage analyses and artificial fertilizations demonstrated a low level of reproductive isolation between species even though size-assortative mating may contribute to isolation. Most parapatric population pairs were strongly differentiated due to the joint effects of geographic distance and barriers to migration. In contrast, we found variable levels of gene flow between sympatric populations ranging from panmixia to moderate differentiation, which indicates a gradient of divergence with some population pairs that may correspond to alternative morphs or ecotypes of a single species and others that remain partially isolated. Ecologically based divergent selection may explain these variable levels of divergence among sympatric population pairs, but incomplete genome swamping following secondary contact could have also played a role. Overall, this study illustrates how highly differentiated phenotypes can be maintained despite high levels of gene flow that limit the progress towards speciation. © 2015 European Society For Evolutionary Biology. Journal of Evolutionary Biology © 2015 European Society For Evolutionary Biology.

  19. Aquatic assessment of the Pike Hill Copper Mine Superfund site, Corinth, Vermont

    Science.gov (United States)

    Piatak, Nadine M.; Argue, Denise M.; Seal, Robert R.; Kiah, Richard G.; Besser, John M.; Coles, James F.; Hammarstrom, Jane M.; Levitan, Denise M.; Deacon, Jeffrey R.; Ingersoll, Christopher G.

    2013-01-01

    The Pike Hill Copper Mine Superfund site in Corinth, Orange County, Vermont, includes the Eureka, Union, and Smith mines along with areas of downstream aquatic ecosystem impairment. The site was placed on the U.S. Environmental Protection Agency (USEPA) National Priorities List in 2004. The mines, which operated from about 1847 to 1919, contain underground workings, foundations from historical structures, several waste-rock piles, and some flotation tailings. The mine site is drained to the northeast by Pike Hill Brook, which includes several wetland areas, and to the southeast by an unnamed tributary that flows to the south and enters Cookville Brook. Both brooks eventually drain into the Waits River, which flows into the Connecticut River. The aquatic ecosystem at the site was assessed using a variety of approaches that investigated surface-water quality, sediment quality, and various ecological indicators of stream-ecosystem health. The degradation of surface-water quality is caused by elevated concentrations of copper, and to a lesser extent cadmium, with localized effects caused by aluminum, iron, and zinc. Copper concentrations in surface waters reached or exceeded the USEPA national recommended chronic water-quality criteria for the protection of aquatic life in all of the Pike Hill Brook sampling locations except for the location farthest downstream, in half of the locations sampled in the tributary to Cookville Brook, and in about half of the locations in one wetland area located in Pike Hill Brook. Most of these same locations also contained concentrations of cadmium that exceeded the chronic water-quality criteria. In contrast, surface waters at background sampling locations were below these criteria for copper and cadmium. Comparison of hardness-based and Biotic Ligand Model (BLM)-based criteria for copper yields similar results with respect to the extent or number of stations impaired for surface waters in the affected area. However, the BLM

  20. AGE DETERMINATION FROM PUBIC SYMPHYSEAL CHANGES- AN AUTOPSY STUDY

    Directory of Open Access Journals (Sweden)

    Jinesh Parambathettu Sasidharan

    2017-02-01

    Full Text Available BACKGROUND Biological identity is one of the first things to be established when analysing skeletal, badly decomposed or otherwise unidentified human remains. The determination of age at death is an important component of identity establishment. Pubic symphysis examination is one of the reliable methods of age estimation. We attempt to determine the age of deceased from pubic symphysis using Suchey-Brooks method and to find the accuracy of Suchey-Brooks method in our population. From among the cases brought for autopsy at Department of Forensic Medicine, Government Medical College, Kottayam, Kerala, from 01/03/2012 to 28/02/2013, 316 cases were included in the study. Their pubic symphysis were harvested. Age determined using SucheyBrooks method and compared with their chronological age. The aim of the study is to- 1. Determine the age of deceased from pubic symphysis using Suchey-Brooks method. 2. Compare the age determined by Suchey-Brooks method and chronological age and find the accuracy of Suchey-Brooks method in our population. MATERIALS AND METHODS Study Design- Descriptive study- Cross-sectional study, Centre of Study- Department of Forensic Medicine, Government Medical College, Kottayam, Study Sample- From the cases brought for medicolegal postmortem examination in the Department of Forensic Medicine, Government Medical College, Kottayam, Kerala, a sample of 316 cases were selected. To make the distribution among various age groups, even the total sample divided for the age group, which resulted in having 60 samples per age group. Age groups were considered as Group 1- Age between 18-25 years; Group 2- Age between 25-35 years; Group 3- Age between 35-45 years; Group 4- Age between 45-55 years; Group 5- Age between 55-65 years. Inclusion Criteria- 1. Cases with known age; 2. Cases whose age between 18 and 65 years. Exclusion Criteria- 1. Unknown cases; 2. Known cases with doubtful age; 3. Individuals having pubic bone trauma involving the

  1. Tephrochronology of the Brooks River Archaeological District, Katmai National Park and Preserve, Alaska: What can and cannot be done with tephra deposits

    Science.gov (United States)

    Riehle, J.R.; Dumond, D.E.; Meyer, C.E.; Schaaf, J.M.

    2000-01-01

    The Brooks River Archaeological District (BRAD) in Katmai National Park and Preserve is a classical site for the study of early humans in Alaska. Because of proximity to the active Aleutian volcanic arc, there are numerous tephra deposits in the BRAD, which are potentially useful for correlating among sites of archaeological investigations. Microprobe analyses of glass separates show, however, that most of these tephra deposits are heterogeneous mixtures of multiple glass populations. Some glasses are highly similar to pyroclasts of Aniakchak Crater (160 km to the south), others are similar to pyroclasts in the nearby Valley of Ten Thousand Smokes, and some are similar to no other tephra samples from the Alaska Peninsula. Moreover, tephra deposits in any one archaeological study site are not always similar to those from nearby sites, indicating inconsistent preservation of these mainly thin, fine-grained deposits. At least 15, late Holocene tephra deposits are inferred at the BRAD. Their heterogeneity is the result of either eruptions of mixed or heterogeneous magmas, like the 1912 Katmai eruption, or secondary mixing of closely succeeding tephra deposits. Because most cannot be reliably distinguished from one another on the basis of megascopic properties, their utility for correlations is limited. At least one deposit can be reliably identified because of its thickness (10 cm) and colour stratification. Early humans seem not to have been significantly affected by these tephra falls, which is not surprising in view of the resilience exhibited by both plants and animals following the 1912 Katmai eruption.

  2. Controls on mercury and methylmercury deposition for two watersheds in Acadia National Park, Maine.

    Science.gov (United States)

    Johnson, K B; Haines, T A; Kahl, J S; Norton, S A; Amirbahman, Aria; Sheehan, K D

    2007-03-01

    Throughfall and bulk precipitation samples were collected for two watersheds at Acadia National Park, Maine, from 3 May to 16 November 2000, to determine which landscape factors affected mercury (Hg) deposition. One of these watersheds, Cadillac Brook, burned in 1947, providing a natural experimental design to study the effects of forest type on deposition to forested watersheds. Sites that face southwest received the highest Hg deposition, which may be due to the interception of cross-continental movement of contaminated air masses. Sites covered with softwood vegetation also received higher Hg deposition than other vegetation types because of the higher scavenging efficiency of the canopy structure. Methyl mercury (MeHg) deposition was not affected by these factors. Hg deposition, as bulk precipitation and throughfall was lower in Cadillac Brook watershed (burned) than in Hadlock Brook watershed (unburned) because of vegetation type and watershed aspect. Hg and MeHg inputs were weighted by season and vegetation type because these two factors had the most influence on deposition. Hg volatilization was not determined. The total Hg deposition via throughfall and bulk precipitation was 9.4 microg/m(2)/year in Cadillac Brook watershed and 10.2 microg/m(2)/year in Hadlock Brook watershed. The total MeHg deposition via throughfall and bulk precipitation was 0.05 microg/m(2)/year in Cadillac Brook watershed and 0.10 microg/m(2)/year in Hadlock Brook watershed.

  3. Influences of spatial and temporal variation on fish-habitat relationships defined by regression quantiles

    Science.gov (United States)

    Dunham, J.B.; Cade, B.S.; Terrell, J.W.

    2002-01-01

    We used regression quantiles to model potentially limiting relationships between the standing crop of cutthroat trout Oncorhynchus clarki and measures of stream channel morphology. Regression quantile models indicated that variation in fish density was inversely related to the width:depth ratio of streams but not to stream width or depth alone. The spatial and temporal stability of model predictions were examined across years and streams, respectively. Variation in fish density with width:depth ratio (10th-90th regression quantiles) modeled for streams sampled in 1993-1997 predicted the variation observed in 1998-1999, indicating similar habitat relationships across years. Both linear and nonlinear models described the limiting relationships well, the latter performing slightly better. Although estimated relationships were transferable in time, results were strongly dependent on the influence of spatial variation in fish density among streams. Density changes with width:depth ratio in a single stream were responsible for the significant (P 80th). This suggests that stream-scale factors other than width:depth ratio play a more direct role in determining population density. Much of the variation in densities of cutthroat trout among streams was attributed to the occurrence of nonnative brook trout Salvelinus fontinalis (a possible competitor) or connectivity to migratory habitats. Regression quantiles can be useful for estimating the effects of limiting factors when ecological responses are highly variable, but our results indicate that spatiotemporal variability in the data should be explicitly considered. In this study, data from individual streams and stream-specific characteristics (e.g., the occurrence of nonnative species and habitat connectivity) strongly affected our interpretation of the relationship between width:depth ratio and fish density.

  4. Salmonid Chromosome Evolution as Revealed by a Novel Method for Comparing RADseq Linkage Maps

    Science.gov (United States)

    Gosselin, Thierry; Normandeau, Eric; Lamothe, Manuel; Isabel, Nathalie; Audet, Céline; Bernatchez, Louis

    2016-01-01

    Whole genome duplication (WGD) can provide material for evolutionary innovation. Family Salmonidae is ideal for studying the effects of WGD as the ancestral salmonid underwent WGD relatively recently, ∼65 Ma, then rediploidized and diversified. Extensive synteny between homologous chromosome arms occurs in extant salmonids, but each species has both conserved and unique chromosome arm fusions and fissions. Assembly of large, outbred eukaryotic genomes can be difficult, but structural rearrangements within such taxa can be investigated using linkage maps. RAD sequencing provides unprecedented ability to generate high-density linkage maps for nonmodel species, but can result in low numbers of homologous markers between species due to phylogenetic distance or differences in library preparation. Here, we generate a high-density linkage map (3,826 markers) for the Salvelinus genera (Brook Charr S. fontinalis), and then identify corresponding chromosome arms among the other available salmonid high-density linkage maps, including six species of Oncorhynchus, and one species for each of Salmo, Coregonus, and the nonduplicated sister group for the salmonids, Northern Pike Esox lucius for identifying post-duplicated homeologs. To facilitate this process, we developed MapComp to identify identical and proximate (i.e. nearby) markers between linkage maps using a reference genome of a related species as an intermediate, increasing the number of comparable markers between linkage maps by 5-fold. This enabled a characterization of the most likely history of retained chromosomal rearrangements post-WGD, and several conserved chromosomal inversions. Analyses of RADseq-based linkage maps from other taxa will also benefit from MapComp, available at: https://github.com/enormandeau/mapcomp/ PMID:28173098

  5. Plating laboratory

    International Nuclear Information System (INIS)

    Seamster, A.G.; Weitkamp, W.G.

    1984-01-01

    The lead plating of the prototype resonator has been conducted entirely in the plating laboratory at SUNY Stony Brook. Because of the considerable cost and inconvenience in transporting personnel and materials to and from Stony Brook, it is clearly impractical to plate all the resonators there. Furthermore, the high-beta resonator cannot be accommodated at Stony Brook without modifying the set up there. Consequently the authors are constructing a plating lab in-house

  6. Impact of Water Usage on the Hydrology of Streams in the Mill River Watershed, Massachusetts

    Science.gov (United States)

    Newton, R. M.; Rhodes, A. L.; Pufall, A.; Bradstreet, E.; Katchpole, S.; Mattison, E.; Woods, R.

    2001-05-01

    Removal of surface water for municipal water supplies has reduced base flow in two tributary streams to the Mill River in Whately Massachusetts. This reduction in the flow of high quality water from these tributaries reduces the amount of dilution of high anthropogenic chemical loads in the main branch of the Mill River leading to high concentrations of chloride and sulfate. The city of Northampton, operates a reservoir on West Brook that removes an average of 5,700 m3/day. West Brook occupies a 28.4 km2 watershed underlain by Paleozoic igneous and metamorphic rocks that are mainly overlain by thin deposits of Pleistocene till. There are isolated areas of stratified drift in the area of the reservoir and where West Brook enters into the area formerly occupied by Glacial Lake Hitchcock. The reservoir (0.35 km2 in area) lies within the upper third of the subcatchment and is primarily fed by Avery Brook (7.6 km2 watershed). Although the reservoirs watershed represent about one third of the West Brook watershed, high water demands limit the release of water from the reservoir to periods of high flow associated with intense rainfall or snowmelt events. A comparison of unit hydrographs from Avery Brook, upstream of the reservoir with those from West Brook near where it enters the Mill River show significant lower discharges downstream (1mm/day). A comparison of flow duration curves show that discharges below the reservoir are dramatically lower during low flow conditions. The town of South Deerfield operates a reservoir on Roaring Brook that removes approximately 3,800 m3/day. Roaring Brook occupies a 14.0 km2 watershed that is similar in geology to West Brook. The reservoir is located on the downstream section of the brook just above where it enters the Mill River. Unlike the Northampton reservoir, water is almost continually released from the reservoir although the rate does fluctuate greatly. Data from a gage station located just downstream of the dam show rapid

  7. Fatty acid composition of fish species with different feeding habits from an Arctic Lake.

    Science.gov (United States)

    Gladyshev, M I; Sushchik, N N; Glushchenko, L A; Zadelenov, V A; Rudchenko, A E; Dgebuadze, Y Y

    2017-05-01

    We compared the composition and content of fatty acids (FAs) in fish with different feeding habits (sardine (least) cisco Coregonus sardinella, goggle-eyed charr (pucheglazka) form of Salvelinus alpinus complex, humpback whitefish Coregonus pidschian, broad whitefish Coregonus nasus, boganid charr Salvelinus boganidae, and northern pike Esox lucius from an Arctic Lake. Feeding habits of the studied fish (planktivore, benthivore, or piscivore) significantly affected the composition of biomarker fatty acids and the ratio of stable isotopes of carbon and nitrogen in their biomass. The hypothesis on a higher content of eicosapentaenoic and docosahexaenoic acids in the fish of higher trophic level (piscivores) when compared within the same taxonomic group (order Salmoniformes) was confirmed.

  8. A report on the fisheries resources of the lower Nelson River and the impacts of hydroelectric development, 1988 data

    International Nuclear Information System (INIS)

    Swanson, G.M.; Kansas, K.R.; Matkowski, S.M.

    1990-01-01

    Fisheries studies on the lower Nelson River (Manitoba) system have had the goals of gaining an understanding of the fisheries resources present, assessing current and potential impacts of hydroelectric developments, and investigating enhancement or mitigative options. In 1988, a resource inventory of McMillan and 12-Mile Creeks was conducted to increase understanding of brook trout stocks in the Limestone River system. Results indicate that both streams contain self-sustaining populations. Baseline data collection in the Conawapa Forebay of the Nelson River was initiated in 1988. Inventories of fish populations were conducted, focusing on lake sturgeon. Three long-term monitoring projects were continued in 1988, investigating the populations of spawning brook trout, larval brook trout, and anadromy in brook trout. Four major tributaries to the Nelson River were classified on the basis of physical and chemical characteristics in an attempt to understand brook trout distribution patterns. Ten sturgeon were captured in Angling Lake in 1988 and fitted with radio tags to assess the importance of the Angling Lake-Angling River system to Nelson River lake sturgeon. To investigate the feasibility of enhancing brook trout populations in the Nelson River system, baskets of eggs were planted in previously identified spawning areas in three creeks in 1988. The eggs developed and hatched only in CN Creek. The potential for rehabilitating the Kettle River brook trout population by transfer of fish from other rivers was also investigated in 1988. Radio-tagged fish remained in the Kettle River-Long Spruce system throughout the life of the tags and appear to have found suitable summer and winter habitat. 60 refs., 76 figs., 38 tabs

  9. Contrasting chemical response to artificial acidification of three acid-sensitive streams in Maine, USA

    International Nuclear Information System (INIS)

    Goss, Heather V.; Norton, Stephen A.

    2008-01-01

    We experimentally acidified three low alkalinity first-order streams in forested catchments in Maine, USA. We evaluated water samples from a reference site above the point of hydrochloric acid addition and from two or three sites located 16 to 94 m downstream. Neutralization included protonation of weak acids, adsorption of sulfate, and ion exchange of base cations and aluminum (Al) for protons (H + ). Protonation of bicarbonate was significant in the relatively high pH Hadlock Brook. Protonation of weak organic acids dominated in the high dissolved organic carbon (DOC) Mud Pond Inlet. The response in low DOC, low pH East Bear Brook was dominated by stream substrate release of cations. East Bear Brook had the strongest acid neutralization response per unit catchment area. In all streams, exchangeable calcium (Ca) and magnesium (Mg) were mobilized, with Ca > Mg. Al was also mobilized. During initial stages of acidification, Ca desorbed preferentially, whereas Al mobilization dominated later. Early in the recovery, adsorption of Ca to the streambed sediments was kinetically favored over adsorption of Al. Though pH increased downstream of acid addition, the streams remained undersaturated with respect to amorphous Al(OH) 3 , so Al did not precipitate. In East Bear Brook, however, Al left solution further downstream through adsorption. This process was likely kinetically controlled, because it occurred in East Bear Brook (3-4 L/s) but did not occur in Hadlock Brook (ca. 40 L/s) or Mud Pond Inlet (ca. 60 L/s). During experimental acidification, the initial Al:Ca ratio of a stream's response may indicate the acidification status of the catchment. Short-term stream acidification experiments illuminate processes characteristic of episodic stream acidification and of long-term catchment acidification. East Bear Brook and Hadlock Brook catchments are in early to intermediate stages of acidification. The Mud Pond Inlet catchment (high Al:Ca ratio) is in a later stage of

  10. Improvement of airfoil trailing edge bluntness noise model

    DEFF Research Database (Denmark)

    Zhu, Wei Jun; Shen, Wen Zhong; Sørensen, Jens Nørkær

    2016-01-01

    In this article, airfoil trailing edge bluntness noise is investigated using both computational aero-acoustic and semi-empirical approach. For engineering purposes, one of the most commonly used prediction tools for trailing edge noise are based on semi-empirical approaches, for example, the Brooks......, Pope, and Marcolini airfoil noise prediction model developed by Brooks, Pope, and Marcolini (NASA Reference Publication 1218, 1989). It was found in previous study that the Brooks, Pope, and Marcolini model tends to over-predict noise at high frequencies. Furthermore, it was observed...

  11. Determination of the effects of fine-grained sediment and other limiting variables on trout habitat for selected streams in Wisconsin

    Science.gov (United States)

    Scudder, Barbara C.; Selbig, J.W.; Waschbusch, R.J.

    2000-01-01

    Two Habitat Suitability Index (HSI) models, developed by the U.S. Fish and Wildlife Service, were used to evaluate the effects of fine-grained (less than 2 millimeters) sediment on brook trout (Salvelinusfontinalis, Mitchill) and brown trout (Salmo trutta, Linnaeus) in 11 streams in west-central and southwestern Wisconsin. Our results indicated that fine-grained sediment limited brook trout habitat in 8 of 11 streams and brown trout habitat in only one stream. Lack of winter and escape cover for fry was the primary limiting variable for brown trout at 61 percent of the sites, and this factor also limited brook trout at several stations. Pool area or quality, in stream cover, streambank vegetation for erosion control, minimum flow, thalweg depth maximum, water temperature, spawning substrate, riffle dominant substrate, and dissolved oxygen also were limiting to trout in the study streams. Brook trout appeared to be more sensitive to the effects of fine-grained sediment than brown trout. The models for brook trout and brown trout appeared to be useful and objective screening tools for identifying variables limiting trout habitat in these streams. The models predicted that reduction in the amount of fine-grained sediment would improve brook trout habitat. These models may be valuable for establishing instream sediment-reduction goals; however, the decrease in sediment delivery needed to meet these goals cannot be estimated without quantitative data on land use practices and their effects on sediment delivery and retention by streams.

  12. Predicting future changes in Muskegon River Watershed game fish distributions under future land cover alteration and climate change scenarios

    Science.gov (United States)

    Steen, Paul J.; Wiley, Michael J.; Schaeffer, Jeffrey S.

    2010-01-01

    Future alterations in land cover and climate are likely to cause substantial changes in the ranges of fish species. Predictive distribution models are an important tool for assessing the probability that these changes will cause increases or decreases in or the extirpation of species. Classification tree models that predict the probability of game fish presence were applied to the streams of the Muskegon River watershed, Michigan. The models were used to study three potential future scenarios: (1) land cover change only, (2) land cover change and a 3°C increase in air temperature by 2100, and (3) land cover change and a 5°C increase in air temperature by 2100. The analysis indicated that the expected change in air temperature and subsequent change in water temperatures would result in the decline of coldwater fish in the Muskegon watershed by the end of the 21st century while cool- and warmwater species would significantly increase their ranges. The greatest decline detected was a 90% reduction in the probability that brook trout Salvelinus fontinalis would occur in Bigelow Creek. The greatest increase was a 276% increase in the probability that northern pike Esox lucius would occur in the Middle Branch River. Changes in land cover are expected to cause large changes in a few fish species, such as walleye Sander vitreus and Chinook salmon Oncorhynchus tshawytscha, but not to drive major changes in species composition. Managers can alter stream environmental conditions to maximize the probability that species will reside in particular stream reaches through application of the classification tree models. Such models represent a good way to predict future changes, as they give quantitative estimates of the n-dimensional niches for particular species.

  13. Shallow bedrock limits groundwater seepage-based headwater climate refugia

    Science.gov (United States)

    Briggs, Martin A.; Lane, John W.; Snyder, Craig D.; White, Eric A.; Johnson, Zachary; Nelms, David L.; Hitt, Nathaniel P.

    2018-01-01

    Groundwater/surface-water exchanges in streams are inexorably linked to adjacent aquifer dynamics. As surface-water temperatures continue to increase with climate warming, refugia created by groundwater connectivity is expected to enable cold water fish species to survive. The shallow alluvial aquifers that source groundwater seepage to headwater streams, however, may also be sensitive to seasonal and long-term air temperature dynamics. Depth to bedrock can directly influence shallow aquifer flow and thermal sensitivity, but is typically ill-defined along the stream corridor in steep mountain catchments. We employ rapid, cost-effective passive seismic measurements to evaluate the variable thickness of the shallow colluvial and alluvial aquifer sediments along a headwater stream supporting cold water-dependent brook trout (Salvelinus fontinalis) in Shenandoah National Park, VA, USA. Using a mean depth to bedrock of 2.6 m, numerical models predicted strong sensitivity of shallow aquifer temperature to the downward propagation of surface heat. The annual temperature dynamics (annual signal amplitude attenuation and phase shift) of potential seepage sourced from the shallow modeled aquifer were compared to several years of paired observed stream and air temperature records. Annual stream water temperature patterns were found to lag local air temperature by ∼8–19 d along the stream corridor, indicating that thermal exchange between the stream and shallow groundwater is spatially variable. Locations with greater annual signal phase lag were also associated with locally increased amplitude attenuation, further suggestion of year-round buffering of channel water temperature by groundwater seepage. Numerical models of shallow groundwater temperature that incorporate regional expected climate warming trends indicate that the summer cooling capacity of this groundwater seepage will be reduced over time, and lower-elevation stream sections may no longer serve as larger

  14. Effects of depletion sampling by standard three-pass pulsed DC electrofishing on blood chemistry parameters of fishes from Appalachian streams

    Science.gov (United States)

    Densmore, Christine L.; Panek, Frank M.

    2013-01-01

    Adverse effects on fishes captured by electrofishing techniques have long been recognized, although the extent of associated physical injury and behavioral alterations are highly variable and dependent on a number of factors. We examined the effects of three-pass pulsed DC (PDC) electrofishing on two salmonid species (Rainbow Trout Oncorhynchus mykiss and Brook Trout Salvelinus fontinalis) and five other genera (Green Sunfish Lepomis cyanellus, Potomac Sculpin Cottus girardi, Fathead Minnow Pimephales promelas, Largemouth Bass Micropterus salmoides, and Channel Catfish Ictalurus punctatus) common to Appalachian streams. We examined the corresponding effects of PDC electroshock on the following physiological indicators of stress and trauma: blood glucose and serum lactate, as well as on other blood chemistry, namely, enzymes, electrolytes, minerals, and proteins. All species demonstrated physiological responses to PDC electroshock, indicated by the biochemical differences in blood parameters in unshocked and shocked groups of fish with or without gross evidence of hemorrhagic trauma. Serum lactate was the most consistent indicator of these effects. Significant differences in whole blood glucose levels were also noted in treatment groups in all species except Green Sunfish, although the patterns observed were not as consistent as for serum lactate. Elevations in the serum enzymes, aspartate aminotransferase and creatine kinase, in the electroshocked fish occurred only in the two salmonid species. In many instances, although blood parameters were elevated in electroshocked fish compared with the unshocked controls for a given species, there were no differences in those levels in electroshocked fish based on the presence of gross hemorrhagic trauma to axial musculature. While some of the blood parameters examined correlated with both the occurrence of electroshock and the resultant tissue injury, there was no apparent link between the altered blood chemistry and

  15. Assessment of Native Salmonids Above Hells Canyon Dam, Idaho, 2002-2003 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Kevin A.; Lamansky, Jr., James A. (Idaho Department of Fish and Game, Boise, ID)

    2004-03-01

    We assessed the relationships between specific stream attributes and Yellowstone cutthroat trout Oncorhynchus clarki bouvieri distribution and biomass at 773 stream reaches (averaging 100 m in length) throughout the Upper Snake River Basin in Idaho, in an effort to identify possible limiting factors. Because limiting factors were expected to vary across the range of cutthroat trout distribution in Idaho, separate logistic and multiple regression models were developed for each of the nine major river drainages to relate stream conditions to occurrence and biomass of cutthroat trout. Adequate stream flow to measure fish and habitat existed at 566 sites, and of those, Yellowstone cutthroat trout were present at 322 sites, while rainbow trout O. mykiss (or rainbow x cutthroat hybrids) and brook trout Salvelinus fontinalis occurred at 108 and 181 sites, respectively. In general, cutthroat trout presence at a specific site within a drainage was associated with a higher percentage of public property, higher elevation, more gravel and less fine substrate, and more upright riparian vegetation. However, there was much variation between drainages in the direction and magnitude of the relationships between stream characteristics and Yellowstone cutthroat trout occurrence and biomass, and in model strength. This was especially true for biomass models, in which we were able to develop models for only five drainages that explained more than 50% of the variation in cutthroat trout biomass. Sample size appeared to affect the strength of the biomass models, with a higher explanation of biomass variation in drainages with lower sample sizes. The occurrence of nonnative salmonids was not strongly related to cutthroat trout occurrence, but their widespread distribution and apparent ability to displace native cutthroat trout suggest they may nevertheless pose the largest threat to long-term cutthroat trout persistence in the Upper Snake River Basin.

  16. Bull trout life history, genetics, habitat needs, and limiting factors in central and northeast Oregon, Annual Report 1995

    International Nuclear Information System (INIS)

    Hemmingsen, A.R.; Buchanan, D.V.; Howell, P.J.

    1996-03-01

    To fulfill one objective of the present study, genetic characteristics of Oregon bull trout will be determined by analysis of mitochondrial and nuclear DNA. During 1995, the authors collected and sampled a total of 1,217 bull trout from 46 streams in the Columbia River Basin. DNA analysis of those samples will be conducted at University of Montana. They primarily sampled juvenile fish near natal areas to increase the likelihood of identifying discrete populations while minimizing risk of injury to large spawners. Fork lengths of all fish sampled ranged from 2.6 to 60.5 cm with a median of 12 cm. Eighty-four percent of all bull trout sampled were less than 19 cm while two percent were larger than 27 cm. Bull trout were collected by several methods, mostly by electrofishing. Eighty-six percent of all bull trout sampled were collected by electrofishing with a programmable waveform electrofisher. They observed injuries caused by electrofishing to 8% of that proportion. Based on preliminary analysis, no waveform combination used appeared less injurious than others. Highest voltages appeared less injurious than some that were lower. Frequency of electrofishing injury was significantly correlated to fork length over the range-from 4 to 26 cm. There were indications for substantial risk for such injury to bull trout larger than 26 cm. Other species found in association with bull trout included chinook salmon Oncorhynchus tshawytscha, mountain whitefish Prosopium williamsoni, rainbow trout Oncorhynchus mykiss, sculpins Cottus spp., cutthroat trout Oncorhynchus clarki, non-native brook trout Salvelinus fontinalis, and tailed frogs Ascaphus truei. Rainbow trout was the species most frequently associated with bull trout. No injury or mortality was observed for any of the associated species captured

  17. Fish thermal habitat current use and simulation of thermal habitat availability in lakes of the Argentine Patagonian Andes under climate change scenarios RCP 4.5 and RCP 8.5.

    Science.gov (United States)

    Vigliano, Pablo H; Rechencq, Magalí M; Fernández, María V; Lippolt, Gustavo E; Macchi, Patricio J

    2018-09-15

    Habitat use in relation to the thermal habitat availability and food source as a forcing factor on habitat selection and use of Percichthys trucha (Creole perch), Oncorhynchus mykiss (rainbow trout), Salmo trutta (brown trout) and Salvelinus fontinalis (brook trout) were determined as well as future potential thermal habitat availability for these species under climate change scenarios Representative Concentration Pathways 4.5 and 8.5. This study was conducted in three interconnected lakes of Northern Patagonia (Moreno Lake system). Data on fish abundance was obtained through gill netting and hydroacoustics, and thermal profiles and fish thermal habitat suitability index curves were used to identify current species-specific thermal habitat use. Surface air temperatures from the (NEX GDDP) database for RCP scenarios 4.5 and 8.5 were used to model monthly average temperatures of the water column up to the year 2099 for all three lakes, and to determine potential future habitat availability. In addition, data on fish diet were used to determine whether food could act as a forcing factor in current habitat selection. The four species examined do not use all the thermally suitable habitats currently available to them in the three lakes, and higher fish densities are not necessarily constrained to their "fundamental thermal niches" sensu Magnuson et al. (1979), as extensive use is made of less suitable habitats. This is apparently brought about by food availability acting as a major forcing factor in habitat selection and use. Uncertainties related to the multidimensionality inherent to habitat selection and climate change imply that fish resource management in Patagonia will not be feasible through traditional incremental policies and strategic adjustments based on short-term predictions, but will have to become highly opportunistic and adaptive. Copyright © 2018 Elsevier B.V. All rights reserved.

  18. Simulated and observed 2010 floodwater elevations in selected river reaches in the Pawtuxet River Basin, Rhode Island

    Science.gov (United States)

    Zarriello, Phillip J.; Olson, Scott A.; Flynn, Robert H.; Strauch, Kellan R.; Murphy, Elizabeth A.

    2014-01-01

    Heavy, persistent rains from late February through March 2010 caused severe flooding that set, or nearly set, peaks of record for streamflows and water levels at many long-term streamgages in Rhode Island. In response to this event, hydraulic models were updated for selected reaches covering about 56 river miles in the Pawtuxet River Basin to simulate water-surface elevations (WSEs) at specified flows and boundary conditions. Reaches modeled included the main stem of the Pawtuxet River, the North and South Branches of the Pawtuxet River, Pocasset River, Simmons Brook, Dry Brook, Meshanticut Brook, Furnace Hill Brook, Flat River, Quidneck Brook, and two unnamed tributaries referred to as South Branch Pawtuxet River Tributary A1 and Tributary A2. All the hydraulic models were updated to Hydrologic Engineering Center-River Analysis System (HEC-RAS) version 4.1.0 using steady-state simulations. Updates to the models included incorporation of new field-survey data at structures, high resolution land-surface elevation data, and updated flood flows from a related study. The models were assessed using high-water marks (HWMs) obtained in a related study following the March– April 2010 flood and the simulated water levels at the 0.2-percent annual exceedance probability (AEP), which is the estimated AEP of the 2010 flood in the basin. HWMs were obtained at 110 sites along the main stem of the Pawtuxet River, the North and South Branches of the Pawtuxet River, Pocasset River, Simmons Brook, Furnace Hill Brook, Flat River, and Quidneck Brook. Differences between the 2010 HWM elevations and the simulated 0.2-percent AEP WSEs from flood insurance studies (FISs) and the updated models developed in this study varied with most differences attributed to the magnitude of the 0.2-percent AEP flows. WSEs from the updated models generally are in closer agreement with the observed 2010 HWMs than with the FIS WSEs. The improved agreement of the updated simulated water elevations to

  19. 40Ar/39Ar Dating of Zn-Pb-Ag Mineralization in the Northern Brooks Range, Alaska

    Science.gov (United States)

    Werdon, Melanie B.; Layer, Paul W.; Newberry, Rainer J.

    2004-01-01

    The 40Ar/39Ar laser step-heating method potentially can be used to provide absolute ages for a number of formerly undatable, low-temperature ore deposits. This study demonstrates the use of this method by determining absolute ages for Zn-Pb-Ag sediment-hosted massive sulfide deposits and vein-breccia occurrences found throughout a 300-km-long, east-west-trending belt in the northern Brooks Range, Alaska. Massive sulfide deposits are hosted by Mississippian to Pennsylvanian(?) black carbonaceous shale, siliceous mudstone, and lesser chert and carbonate turbidites of the Kuna Formation (e.g., Red Dog, Anarraaq, Lik (Su), and Drenchwater). The vein-breccia occurrences (e.g., Husky, Story Creek, West Kivliktort Mountain, Vidlee, and Kady) are hosted by a deformed but only weakly metamorphosed package of Upper Devonian to Lower Mississippian mixed continental and marine clastic rocks (the Endicott Group) that stratigraphically underlie the Kuna Formation. The vein-breccias are mineralogically similar to, but not spatially associated with, known massive sulfide deposits. The region's largest shale-hosted massive sulfide deposit is Red Dog; it has reserves of 148 Mt grading 16.6 percent zinc, 4.5 percent lead, and 77 g of silver per tonne. Hydrothermally produced white mica in a whole-rock sample from a sulfide-bearing igneous sill within the Red Dog deposit yielded a plateau age of 314.5 Ma. The plateau age of this whole-rock sample records the time at which temperatures cooled below the argon closure temperature of the white mica and is interpreted to represent the minimum age limit for massive sulfide-related hydrothermal activity in the Red Dog deposit. Sulfide-bearing quartz veins at Drenchwater crosscut a hypabyssal intrusion with a maximum biotite age of 337.0 Ma. Despite relatively low sulfide deposition temperatures in the vein-breccia occurrences (162°-251°C), detrital white mica in sandstone immediately adjacent to large vein-breccia zones was partially to

  20. Funnel Libraries for Real-Time Robust Feedback Motion Planning

    Science.gov (United States)

    2016-07-21

    control inputs to the SBach are raw servo commands to the control surfaces (ailerons, rudder, elevator) and a raw throttle setting. These commands are...Convex Optimization. Cambridge University Press . [Brooks, 1982] Brooks, R. (1982). Symbolic error analysis and robot planning. The International Journal

  1. Human-Swarm Interactions Based on Managing Attractors

    Science.gov (United States)

    2014-03-06

    Laboratory/ Brigham Young University Information Directorate Provo, UT 84602 Rome Research Site/ RISC 525 Brooks Road Rome NY 13441...Information Directorate Rome Research Site/ RISC 525 Brooks Road Rome NY 13441-4505 10. SPONSOR/MONITOR’S ACRONYM(S) AFRL/RI 11. SPONSORING

  2. Hydrothermal, biogenic, and seawater components in metalliferous black shales of the Brooks Range, Alaska: Synsedimentary metal enrichment in a carbonate ramp setting

    Science.gov (United States)

    Slack, John F.; Selby, David; Dumoulin, Julie A.

    2015-01-01

    Trace element and Os isotope data for Lisburne Group metalliferous black shales of Middle Mississippian (early Chesterian) age in the Brooks Range of northern Alaska suggest that metals were sourced chiefly from local seawater (including biogenic detritus) but also from externally derived hydrothermal fluids. These black shales are interbedded with phosphorites and limestones in sequences 3 to 35 m thick; deposition occurred mainly on a carbonate ramp during intermittent upwelling under varying redox conditions, from suboxic to anoxic to sulfidic. Deposition of the black shales at ~335 Ma was broadly contemporaneous with sulfide mineralization in the Red Dog and Drenchwater Zn-Pb-Ag deposits, which formed in a distal marginal basin.Relative to the composition of average black shale, the metalliferous black shales (n = 29) display large average enrichment factors (>10) for Zn (10.1), Cd (11.0), and Ag (20.1). Small enrichments (>2–rare earth elements except Ce, Nd, and Sm. A detailed stratigraphic profile over 23 m in the Skimo Creek area (central Brooks Range) indicates that samples from at and near the top of the section, which accumulated during a period of major upwelling and is broadly correlative with the stratigraphic levels of the Red Dog and Drenchwater Zn-Pb-Ag deposits, have the highest Zn/TOC (total organic carbon), Cu/TOC, and Tl/TOC ratios for calculated marine fractions (no detrital component) of these three metals.Average authigenic (detrital-free) contents of Mo, V, U, Ni, Cu, Cd, Pb, Ge, Re, Se, As, Sb, Tl, Pd, and Au show enrichment factors of 4.3 × 103 to 1.2 × 106 relative to modern seawater. Such moderate enrichments, which are common in other metalliferous black shales, suggest wholly marine sources (seawater and biogenic material) for these metals, given similar trends for enrichment factors in organic-rich sediments of modern upwelling zones on the Namibian, Peruvian, and Chilean shelves. The largest enrichment factors for Zn and Ag

  3. Sleep walking

    Science.gov (United States)

    ... Sleepwalking. In: Chokroverty S, Thomas RJ, eds. Atlas of Sleep Medicine . 2nd ed. Philadelphia, PA: Elsevier Saunders; 2014:380- ... of Clinical Neurology, SUNY Stony Brook, School of Medicine, Stony Brook, NY. Review provided by VeriMed Healthcare ... NIH MedlinePlus Magazine Read more ...

  4. in_focus - Water: Local-level Management | IDRC - International ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    ... in the availability and supply of fresh water are truly a matter of life and death, and ... David B. Brooks is a specialist in natural resources who works with the ... of Energy Conservation, Dr Brooks worked for 6 years with Friends of the Earth ...

  5. New results for virial coefficients of hard spheres in D dimensions

    Indian Academy of Sciences (India)

    Nathan Clisby1 Barry M McCoy2. ARC Centre of Excellence for Mathematics and Statistics of Complex Systems, 139 Barry Street, The University of Melbourne, Victoria 3010, Australia; C.N. Yang Institute for Theoretical Physics, Stony Brook University, Stony Brook, NY 11794-3840, USA ...

  6. Aquatic assessment of the Ely Copper Mine Superfund site, Vershire, Vermont

    Science.gov (United States)

    Seal, Robert R.; Kiah, Richard G.; Piatak, Nadine M.; Besser, John M.; Coles, James F.; Hammarstrom, Jane M.; Argue, Denise M.; Levitan, Denise M.; Deacon, Jeffrey R.; Ingersoll, Christopher G.

    2010-01-01

    The Ely Mine, which operated from 1821 to 1905, and its area of downstream impact constitute the Ely Copper Mine Superfund site. The site was placed on the National Priorities List in 2001. The mine comprises underground workings, foundations from historical structures, several waste-rock piles, roast beds associated with the smelting operation, and slag piles resulting from the smelting. The mine site is drained by Ely Brook, which includes several tributaries, one of which drains a series of six ponds. Ely Brook empties into Schoolhouse Brook, which flows 3.3 kilometers and joins the Ompompanoosuc River.

  7. Photometric Analysis of the Jovian Ring System and Modeling of Ring Origin and Evolution

    Science.gov (United States)

    Esposito, L. W.

    2003-01-01

    We have successfully completed the work described in our proposal. The work supported by this grant resulted in the publication of the following paper: Brooks, S. M., L. W. Esposito, M. R. Showalter, and H. B. Throop. 2002. The size distribution of Jupiter's main ring from Galileo imaging and spectroscopy. Icarus, in press. This was also the major part of Dr. Shawn Brooks PhD dissertation. Dr. Brooks gave oral presentations on this work at the Lunar and Planetary Conference, the annual meetings of the Division for Planetary Sciences of the American Astronomical Society, the annual meetings of the European Geophysical Society, the international Jupiter Conference in Boulder, the Jupiter after Galileo and Cassini Conference in Lisbon and to the Working Group in Non-Linear Dynamics in Potsdam, Germany. This work was reviewed in: Esposito, L. W. 2002. Planetary rings. Rep. hog. Phys. 65, 1741-1783. Planetary rings. LASP reprint 874. Online at http://stacks.iop.org/RoPP/65/1741. Dr. Esposito gave presentations at schools and over the internet on the results of this work. Dr. Brooks lectured in undergraduate and graduate classes on Jupiter's rings, and on the meaning of his research. In August 2003, Dr. Shawn Brooks received the Phd degree from the University of Colorado in Astrophysical and Planetary Sciences.

  8. The Waterviz: The Confluence of Science, Art and Music Illuminates Pattern and Process in Water Cycle Data

    Science.gov (United States)

    Rustad, L.; Martin, M.; Cortada, X.; Quinn, M.; Garlick, S.; Casey, M.; Green, M. B.

    2017-12-01

    The WaterViz for Hubbard Brook is a new online tool for creatively communicating water cycle science to a broad audience with real time hydrologic data. Interfacing between the hydrologic sciences, visual arts, music, education, and graphic design, the WaterViz for Hubbard Brook builds on a new generation of digital environmental sensors and wireless communication devices that are revolutionizing how scientists `see' the natural world. In a nutshell, hydrologic data are captured from small first order catchments at the Hubbard Brook Experimental Forest, NH using an array of environmental sensors. These data are transmitted to the internet in real time and are used to drive a computer model that calculates all components of the water cycle for the catchment in real time. These data, in turn, drive an artistic simulation (delivered as a flash animation) and musical sonification (delivered via an internet radio station) of the water cycle,accurately reflecting the hydrologic processes occurring at that moment in time. The WaterViz for Hubbard Brook provides a unique and novel approach to interactively and intuitively engage the viewer with vast amount of data and information on water cycle science. The WaterViz for Hubbard Brook is available at: https://waterviz.org.

  9. The Influence of fold and fracture development on reservoir behavior of the Lisburne Group of northern Alaska

    Energy Technology Data Exchange (ETDEWEB)

    Wesley K. Wallace; Catherine L. Hanks; Jerry Jensen: Michael T. Whalen; Paul Atkinson; Joseph Brinton; Thang Bui; Margarete Jadamec; Alexandre Karpov; John Lorenz; Michelle M. McGee; T.M. Parris; Ryan Shackleton

    2004-07-01

    The Carboniferous Lisburne Group is a major carbonate reservoir unit in northern Alaska. The Lisburne is folded and thrust faulted where it is exposed throughout the Brooks Range, but is relatively undeformed in areas of current production in the subsurface of the North Slope. The objectives of this study were to develop a better understanding of four major aspects of the Lisburne: (1) The geometry and kinematics of folds and their truncation by thrust faults. (2) The influence of folding on fracture patterns. (3) The influence of deformation on fluid flow. (4) Lithostratigraphy and its influence on folding, faulting, fracturing, and reservoir characteristics. Symmetrical detachment folds characterize the Lisburne in the northeastern Brooks Range. In contrast, Lisburne in the main axis of the Brooks Range is deformed into imbricate thrust sheets with asymmetrical hangingwall anticlines and footwall synclines. The Continental Divide thrust front separates these different structural styles in the Lisburne and also marks the southern boundary of the northeastern Brooks Range. Field studies were conducted for this project during 1999 to 2001 in various locations in the northeastern Brooks Range and in the vicinity of Porcupine Lake, immediately south of the Continental Divide thrust front. Results are summarized below for the four main subject areas of the study.

  10. Michael F. Crowley | NREL

    Science.gov (United States)

    Pittsburgh Supercomputing Center, and at The Scripps Research Institute with David Case and Charles Brooks NIH-funded collaboration with Professor Charles L. Brooks III at the University of Michigan Areas of to determine source of twisting in hydrogen bonding patterns. This modeling will help to understand

  11. In this section of Resonance, we invite readers to pose questions ...

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 23; Issue 3 ... Department of Materials Science and Chemical Engineering Stony Brook University, Stony Brook, NY 11794, USA; Department of Physics and Physical Oceanography Memorial University St. John's NL Canada AIB 3X7; Department of ...

  12. Limited Bandwidth Recognition of Collective Behaviors in Bio-Inspired Swarms

    Science.gov (United States)

    2014-05-09

    RISC 525 Brooks Road Rome NY 13441-4505 8. PERFORMING ORGANIZATION REPORT NUMBER N/A 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS...ES) Air Force Research Laboratory/Information Directorate Rome Research Site/ RISC 525 Brooks Road Rome NY 13441-4505 10. SPONSOR/MONITOR’S ACRONYM

  13. Improvement of airfoil trailing edge bluntness noise model

    Directory of Open Access Journals (Sweden)

    Wei Jun Zhu

    2016-02-01

    Full Text Available In this article, airfoil trailing edge bluntness noise is investigated using both computational aero-acoustic and semi-empirical approach. For engineering purposes, one of the most commonly used prediction tools for trailing edge noise are based on semi-empirical approaches, for example, the Brooks, Pope, and Marcolini airfoil noise prediction model developed by Brooks, Pope, and Marcolini (NASA Reference Publication 1218, 1989. It was found in previous study that the Brooks, Pope, and Marcolini model tends to over-predict noise at high frequencies. Furthermore, it was observed that this was caused by a lack in the model to predict accurately noise from blunt trailing edges. For more physical understanding of bluntness noise generation, in this study, we also use an advanced in-house developed high-order computational aero-acoustic technique to investigate the details associated with trailing edge bluntness noise. The results from the numerical model form the basis for an improved Brooks, Pope, and Marcolini trailing edge bluntness noise model.

  14. 77 FR 45262 - Final Flood Elevation Determinations

    Science.gov (United States)

    2012-07-31

    ..., 450 Bastion Road, Halifax, PA 17032. Township of Lower Paxton Maps are available for inspection at the... downstream side of +609 Borough of Tunkhannock, the railroad bridge. Township of Tunkhannock. Approximately 0.7 mile +655 upstream of Bridge Street. Tributary No. 1 to Swale Brook......... At the Swale Brook...

  15. Forsmark site investigation. Monitoring of brook water levels, electrical conductivities, temperatures and discharges January-December 2009

    Energy Technology Data Exchange (ETDEWEB)

    Johansson, Per-Olof (Artesia Grundvattenkonsult (Sweden)); Juston, John (Juston Konsult (Sweden))

    2011-03-15

    This document reports the monitoring of water levels, electrical conductivities, temperatures and discharges at four brook discharge gauging stations, and the monitoring of water electrical conductivity at the outlet of Lake Bolundsfjaerden in the Forsmark area. The report presents data from 1 January through 31 December 2009 and is a continuation of reporting from Johansson and Juston (2007, 2009), which covered the periods from 1 April 2004 through 31 March 2007 and 1 April 2007 through 31 December 2008, respectively. Long-throated flumes equipped with automatically recording devices were used for the discharge measurements. Every c. 14 days the water depths at the upstream edge of the flumes were measured manually by a ruler as a check. Electrical conductivity and temperature were automatically recorded and these parameters were also measured manually every c. 14 days with the site investigation field devices. SKB's Hydro Monitoring System (HMS) was used to collect and store all data. From HMS quality assured data were transferred to SKB's primary database Sicada. Measurements of levels, electrical conductivities and temperatures were made every 10 minutes (every 30 minutes for electrical conductivity at the outlet of Lake Bolundsfjaerden). For the calculation of discharge, quality assured water level data from the flumes were used. The calculation procedure included consolidation of the time series to hourly averages, screening of data for removal of short-term spikes, noise and other data that were judged erroneous. After the calculations were performed, the results were delivered to Sicada. The amplitudes of water level variations during this reporting period were 0.26-0.33 m at the four stations. The mean electrical conductivities varied between 26 and 41 mS/m at the four discharge stations. The electrical conductivity at the outlet of Lake Bolundsfjaerden varied between 53 and 188 mS/m during the period with the higher values at the end of the

  16. Forsmark site investigation. Monitoring of brook water levels, electrical conductivities, temperatures and discharges January-December 2010

    Energy Technology Data Exchange (ETDEWEB)

    Johansson, Per-Olof (Artesia Grundvattenkonsult (Sweden)); Juston, John (Juston Konsult (Sweden))

    2011-06-15

    This document reports the monitoring of water levels, electrical conductivities, temperatures and discharges at four brook discharge gauging stations, and the monitoring of water electrical conductivity at the outlet of Lake Bolundsfjaerden in the Forsmark area. The report presents data from 1 January through 31 December 2010 and is a continuation of reporting from Johansson and Juston (2007, 2009, 2011), which covered the periods from 1 April 2004 through 31 March 2007, 1 April 2007 through 31 December 2008, and 1 January through 31 December 2009, respectively. Long-throated flumes equipped with automatically recording devices were used for the discharge measurements. Every c. 14 days the water depths at the upstream edge of the flumes were measured manually by a ruler as a check. Electrical conductivity and temperature were automatically recorded and these parameters were also measured manually every c. 14 days with the site investigation field devices. SKB's Hydro Monitoring System (HMS) was used to collect and store all data. From HMS quality assured data were transferred to SKB's primary database Sicada. Measurements of levels, electrical conductivities and temperatures were made every 10 minutes (every 30 minutes for electrical conductivity at the outlet of Lake Bolundsfjaerden). For the calculation of discharge, quality assured water level data from the flumes were used. The calculation procedure included consolidation of the time series to hourly averages, screening of data for removal of short-term spikes, noise and other data that were judged erroneous. After the calculations were performed, the results were delivered to Sicada. The amplitudes of water level variations during this reporting period were 0.41-0.55 m and the mean electrical conductivities varied between 23 and 39 mS/m at the four discharge stations. However, due to mal-function of measuring devices for electrical conductivity, data were missing for relatively long time periods. Due

  17. What are the toxicological effects of mercury in Arctic biota?

    DEFF Research Database (Denmark)

    Dietz, Rune; Sonne, Christian; Basu, Niladri

    2013-01-01

    effects. Species whose concentrations exceed threshold values include the polar bears (Ursus maritimus), beluga whale (Delphinapterus leucas), pilot whale (Globicephala melas), hooded seal (Cystophora cristata), a few seabird species, and landlocked Arctic char (Salvelinus alpinus). Toothed whales appear...

  18. 75 FR 1114 - Notice of Final Federal Agency Actions on Proposed Highway in California

    Science.gov (United States)

    2010-01-08

    ... improve the safety of State Route 16 between the town of Brooks and Interstate 505 in Yolo County by... relate to a proposed highway project, State Route 16 between the town of Brooks and Interstate 505 in the County Yolo, State of California. Those actions grant licenses, permits, and approvals for the project...

  19. Maternal Employment and Child Cognitive Outcomes: The Importance of Analytic Approach

    Science.gov (United States)

    Burchinal, Margaret R.; Clarke-Stewart, K. Alison

    2007-01-01

    J. Brooks-Gunn, W. J. Han, and J. Waldfogel (2002) and the National Institute of Child Health and Human Development Early Child Care Research Network (ECCRN; 2000b) came to different conclusions about the effects of maternal employment--although they were addressing similar questions using the same data set. Brooks-Gunn et al. concluded that…

  20. Anthropogenic acidification of running water in the Kaufunger Forest

    Energy Technology Data Exchange (ETDEWEB)

    Matthias, U; Meinel, W

    1982-01-01

    The acidification of running water in Central Europe caused by acid precipitation and other factors like land-use changes and cation exchange is highly aggravating in the Kaufunger Forest, a woody red sandstone area near Kassel (Federal Republic of Germany). Only 37.5% of the little brooks coming from the Kaufunger Wald had pH values within the neutral range (mean pH > 6.5); 19.6% of the analysed waters were in the pH range of 5.5-6.5, and 25.0% were in the range of 4.5-5.5; 17.9% had pH values below 4.5. The lowest pH value measured in a little brook near the spring was 3.7. The acidification has a strong influence on the aquatic ecosystem. The lowest tolerance against acidification was noticed for mayflies and freshwater shrimps (Gammarus fossarum, G. pulex), which occurred in all brooks with pH values > 6.5. In brooks with pH values below 6.0 these species disappeared, and other species like Nemurella pictetii, Nemoura cinerea, Protonemura auberti and Plectrocnemia conspersa could increase.

  1. A synthesis of ecological and fish-community changes in Lake Ontario, 1970-2000

    Science.gov (United States)

    Mills, E.L.; Casselman, J.M.; Dermott, R.; Fitzsimons, J.D.; Gal, G.; Holeck, K. T.; Hoyle, J.A.; Johannsson, O.E.; Lantry, B.F.; Makarewicz, J.C.; Millard, E.S.; Munawar, I.F.; Munawar, M.; O'Gorman, R.; Owens, R.W.; Rudstam, L. G.; Schaner, T.; Stewart, T.J.

    2005-01-01

    We assessed stressors associated with ecological and fishcommunity changes in Lake Ontario since 1970, when the first symposium on Salmonid Communities in Oligotrophic Lakes (SCOL I) was held (J. Fish. Res. Board Can. 29: 613-616). Phosphorus controls implemented in the early 1970s were undeniably successful; lower food-web studies showed declines in algal abundance and epilimnetic zooplankton production and a shift in pelagic primary productivity toward smaller organisms. Stressors on the fish community prior to 1970 such as exploitation, sea lamprey (Petromyzon marinus) predation, and effects of nuisance populations of alewife (Alosa pseudoharengus) were largely ameliorated by the 1990s. The alewife became a pivotal species supporting a multi-million-dollar salmonid sport fishery, but alewife-induced thiamine deficiency continued to hamper restoration and sustainability of native lake trout (Salvelinus namaycush). Expanding salmonine populations dependent on alewife raised concerns about predator demand and prey supply, leading to reductions in salmonine stocking in the early 1990s. Relaxation of the predation impact by alewives and their shift to deeper water allowed recovery of native fishes such as threespine stickleback (Gasterosteus aculeatus) and emerald shiner (Notropis atherinoides). The return of the Lake Ontario ecosystem to historical conditions has been impeded by unplanned introductions. Establishment of Dreissena spp. led to increased water clarity and increased vectoring of lower trophic-level production to benthic habitats and contributed to the collapse of Diporeia spp. populations, behavioral modifications of key fish species, and the decline of native lake whitefish (Coregonus clupeaformis). Despite reduced productivity, exotic-species introductions, and changes in the fish community, offshore Mysis relicta populations remained relatively stable. The effects of climate and climate change on the population abundance and dynamics of Lake Ontario

  2. Changing numbers of spawning cutthroat trout in tributary streams of Yellowstone Lake and estimates of grizzly bears visiting streams from DNA

    Science.gov (United States)

    Haroldson, M.A.; Gunther, K.A.; Reinhart, Daniel P.; Podruzny, S.R.; Cegelski, C.; Waits, L.; Wyman, T.C.; Smith, J.

    2005-01-01

    Spawning Yellowstone cutthroat trout (Oncorhynchus clarki) provide a source of highly digestible energy for grizzly bears (Ursus arctos) that visit tributary streams to Yellowstone Lake during the spring and early summer. During 1985–87, research documented grizzly bears fishing on 61% of the 124 tributary streams to the lake. Using track measurements, it was estimated that a minimum of 44 grizzly bears fished those streams annually. During 1994, non-native lake trout (Salvelinus namaycush) were discovered in Yellowstone Lake. Lake trout are efficient predators and have the potential to reduce the native cutthroat population and negatively impact terrestrial predators that use cutthroat trout as a food resource. In 1997, we began sampling a subset of streams (n = 25) from areas of Yellowstone Lake surveyed during the previous study to determine if changes in spawner numbers or bear use had occurred. Comparisons of peak numbers and duration suggested a considerable decline between study periods in streams in the West Thumb area of the lake. The apparent decline may be due to predation by lake trout. Indices of bear use also declined on West Thumb area streams. We used DNA from hair collected near spawning streams to estimate the minimum number of bears visiting the vicinity of spawning streams. Seventy-four individual bears were identified from 429 hair samples. The annual number of individuals detected ranged from 15 in 1997 to 33 in 2000. Seventy percent of genotypes identified were represented by more than 1 sample, but only 31% of bears were documented more than 1 year of the study. Sixty-two (84%) bears were only documented in 1 segment of the lake, whereas 12 (16%) were found in 2–3 lake segments. Twenty-seven bears were identified from hair collected at multiple streams. One bear was identified on 6 streams in 2 segments of the lake and during 3 years of the study. We used encounter histories derived from DNA and the Jolly-Seber procedure in Program MARK

  3. Comparisons of likelihood and machine learning methods of individual classification

    Science.gov (United States)

    Guinand, B.; Topchy, A.; Page, K.S.; Burnham-Curtis, M. K.; Punch, W.F.; Scribner, K.T.

    2002-01-01

    Classification methods used in machine learning (e.g., artificial neural networks, decision trees, and k-nearest neighbor clustering) are rarely used with population genetic data. We compare different nonparametric machine learning techniques with parametric likelihood estimations commonly employed in population genetics for purposes of assigning individuals to their population of origin (“assignment tests”). Classifier accuracy was compared across simulated data sets representing different levels of population differentiation (low and high FST), number of loci surveyed (5 and 10), and allelic diversity (average of three or eight alleles per locus). Empirical data for the lake trout (Salvelinus namaycush) exhibiting levels of population differentiation comparable to those used in simulations were examined to further evaluate and compare classification methods. Classification error rates associated with artificial neural networks and likelihood estimators were lower for simulated data sets compared to k-nearest neighbor and decision tree classifiers over the entire range of parameters considered. Artificial neural networks only marginally outperformed the likelihood method for simulated data (0–2.8% lower error rates). The relative performance of each machine learning classifier improved relative likelihood estimators for empirical data sets, suggesting an ability to “learn” and utilize properties of empirical genotypic arrays intrinsic to each population. Likelihood-based estimation methods provide a more accessible option for reliable assignment of individuals to the population of origin due to the intricacies in development and evaluation of artificial neural networks. In recent years, characterization of highly polymorphic molecular markers such as mini- and microsatellites and development of novel methods of analysis have enabled researchers to extend investigations of ecological and evolutionary processes below the population level to the level of

  4. The structuring role of fish in Greenland lakes: an overview based on contemporary and paleoecological studies of 87 lakes from the low and the high Arctic

    DEFF Research Database (Denmark)

    Jeppesen, Erik; Lauridsen, Torben L.; Christoffersen, Kirsten S.

    2017-01-01

    largest between fishless lakes and lakes hosting only sticklebacks (Gasterosteus aculeatus), while lakes with both Arctic charr (Salvelinus arcticus) and stickleback revealed a more modest response, indicating that presence of charr modulates the predation effect of sticklebacks. It is predicted that more...

  5. Fish hydroacoustic survey standardization: A step forward based on comparisons of methods and systems from vertical surveys of a large deep lake

    Czech Academy of Sciences Publication Activity Database

    Draštík, Vladislav; Godlewska, M.; Balk, H.; Clabburn, P.; Kubečka, Jan; Morrissey, E.; Hateley, J.; Winfield, I. J.; Mrkvička, Tomáš; Guillard, J.

    2017-01-01

    Roč. 15, č. 10 (2017), s. 836-846 ISSN 1541-5856 Institutional support: RVO:60077344 Keywords : charr salvelinus-alpinus * great-lakes * biomass * uk * populations Subject RIV: EH - Ecology, Behaviour OBOR OECD: Marine biology, freshwater biology, limnology Impact factor: 1.992, year: 2016

  6. Risk analysis of exotic fish species included in the Dutch Fisheries Act and their hybrids

    NARCIS (Netherlands)

    Schiphouwer, M.E.; Kessel, van N.; Matthews, J.; Leuven, R.S.E.W.; Koppel, S.; Kranenbarg, J.; Haenen, O.L.M.; Lenders, H.J.R.; Nagelkerke, L.A.J.; Velde, van der G.; Crombaghs, B.; Zollinger, R.

    2014-01-01

    In dit rapport worden de risico’s geanalyseerd van exotische vissoorten die zijn opgenomen in de Visserijwet en hun hybriden. De volgende soorten en één specifieke hybride zijn in de analyse meegenomen: beekridder (Salvelinus alpinus); roofblei (Leuciscus aspius); karper (Cyprinus carpio);

  7. Wide Area Recovery and Resiliency Program (WARRP) Knowledge Enhancement Events: CBR Workshop After Action Report

    Science.gov (United States)

    2012-01-01

    National Guard Graham Richard EPA Region VIII Greenwalt Robert Lawrence Livermore National Laboratory Hard Dave Colorado Department of Emergency...Jefferson County Office of Emergency Management Smedley Chuck Denver Public Health Stein Steve Pacific Northwest National Laboratory Steinhour LeeAnn...Laboratory Brooke Buddemeier brooke2@llnl.gov Chris Campbell campbell48@llnl.gov Robert Greenwalt greenwalt1@llnl.gov Wilthea Hibbard hibbard3

  8. Concentrations and content of mercury in bark, wood, and leaves in hardwoods and conifers in four forested sites in the northeastern USA

    Science.gov (United States)

    Yang Yang; Ruth D. Yanai; Charles T. Driscoll; Mario Montesdeoca; Kevin T. Smith

    2018-01-01

    Mercury (Hg) is deposited from the atmosphere to remote areas such as forests, but the amount of Hg in trees is not well known. To determine the importance of Hg in trees, we analyzed foliage, bark and bole wood of eight tree species at four sites in the northeastern USA (Huntington Forest, NY; Sleepers River, VT; Hubbard Brook, NH; Bear Brook, ME). Foliar...

  9. Snow and frost measurements in a watershed-management research program

    Science.gov (United States)

    Richard S. Sartz

    1957-01-01

    I am going to tell you about our snow and frost work on the Hubbard Brook Experimental Forest in the White Mountains of New Hampshire. Hubbard Brook is one of several experimental areas scattered throughout the country on which personnel of the United States Forest Service are seeking to learn how different kinds of forests and methods of managing them affect...

  10. Energy at Stony Brook.

    Science.gov (United States)

    Visich, Marian, Jr.

    1984-01-01

    Discusses strategies used in a course for nonengineering students which consists of case studies of such sociotechnological problems as automobile safety, water pollution, and energy. Solutions to the problems are classified according to three approaches: education, government regulation, and technological fix. (BC)

  11. Dynamics of dissolved organic matter during four storm events in two forest streams: source, export, and implications for harmful disinfection byproduct formation.

    Science.gov (United States)

    Yang, Liyang; Hur, Jin; Lee, Sonmin; Chang, Soon-Woong; Shin, Hyun-Sang

    2015-06-01

    Dynamics of river dissolved organic matter (DOM) during storm events have profound influences on the downstream aquatic ecosystem and drinking water safety. This study investigated temporal variations in DOM during four storm events in two forest headwater streams (the EH and JH brooks, South Korea) and the impacts on the disinfection byproducts (DBPs) formation potential. The within-event variations of most DOM quantity parameters were similar to the flow rate in the EH but not in the larger JH brook. The dissolved organic carbon (DOC) showed clockwise and counterclockwise hysteresis with the flow rate in the EH and JH brooks, respectively, indicating the importance of both flow path and DOM source pool size in determining the effects of storm events. The stream DOM became less aromatic/humified from the first to the last event in both brooks, probably due to the increasing fresh plant pool and the decreasing leaf litter pool during the course of rainy season. The DOC export during each event increased 1.3-2.7- and 1.1-7.0-fold by stormflows in the EH and JH brooks, respectively. The leaf litter and soil together was the major DOM source, particularly during early events. The enhanced DOM export probably increases the risks of DBPs formation in disinfection, as indicated by a strong correlation observed between DOC and trihalomethanes formation potential (THMFP). High correlations between two humic-like fluorescent components and THMFP further suggested the potential of assessing THMFP with in situ fluorescence sensors during storms.

  12. Nucleon Decay and Neutrino Experiments, Experiments at High Energy Hadron Colliders, and String Theor

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Chang Kee [State University of New York at Stony Brook; Douglas, Michaek [State University of New York at Stony Brook; Hobbs, John [State University of New York at Stony Brook; McGrew, Clark [State University of New York at Stony Brook; Rijssenbeek, Michael [State University of New York at Stony Brook

    2013-07-29

    This is the final report of the DOE grant DEFG0292ER40697 that supported the research activities of the Stony Brook High Energy Physics Group from November 15, 1991 to April 30, 2013. During the grant period, the grant supported the research of three Stony Brook particle physics research groups: The Nucleon Decay and Neutrino group, the Hadron Collider Group, and the Theory Group.

  13. Salmon returns and consumer fitness: growth and energy storage in stream-dwelling salmonids increases with spawning salmon abundance

    Science.gov (United States)

    We examined how biomass of marine-derived nutrients (MDN), in the form of spawning Pacific salmon, influenced the nutritional status and nitrogen stable isotope ratios (d15N) of stream-dwelling fishes. We sampled coho salmon (Oncorhynchus kisutch) parr and juvenile Dolly Varden (Salvelinus malma) d...

  14. Acidification of waters in the locality of Smolník deposit

    Directory of Open Access Journals (Sweden)

    Geldová Erika

    2000-09-01

    Full Text Available The decrease in pH of natural waters, or in other words acidification of waters, and consequent acidification of soil is a direct consequence of intensive oxidation of sulphides in mines and mining wastes. Mining water can be extremely acidic and leaches or dissolves the rock ore minerals more intensively than common surface or underground water. Such water may transfer various elements in a dissolved form. In addition to common alkaline elements and metals of alkaline earth, water mobilises heavy metals, as well as elements like Al and As. Smolník deposit is one of localities, where the unfavourable influence of acidic water on the surface water can be observed. The analysis of water in the deserted mine and in the broader area surrounding this mine was made after the ecological accident in the Smolník brook in 1995. In addition to a dominant content of sulphates, this water has a high content of Fe and Al and other metals, such as Mg, Zn, Cu and Mn. The quality of brook water has improved – decrease in the content of dissolved substances (tab.2. However, brook water is still very acidic, pH is lower than 6, what is a limiting value for surface water. The above-mentioned changes occurred due to adjustment of regime of mining water that took place after 1996. This adjustment caused a decrease in the quantity of leaking mining water into the brook. A new drainage discharging into the brook on the place of former shaft Charitas was built, but still brings contaminated waters. The total quantity of dissolved substances (sulphates, Fe, Al, Cu, Zn decreased in the brook water in comparison with that in 1995. Water flowing out of mine through a new drainage is not only very acidic, but also contains a lot of sulphates and Fe, Al, Mg elements. Whether this favourable progress will continue or not, it will be shown by further monitoring of this territory.

  15. First record of Paralemanea torulosa (Roth Sheath & A.R. Sherwood and new findings of Lemanea fluviatilis (Linnaeus C. Ag. and Hildenbrandia rivularis (Liebmann J. Agardh (Rhodophyta in Serbia

    Directory of Open Access Journals (Sweden)

    Blagojević Ana

    2017-04-01

    Full Text Available The paper presents the first record of the freshwater red alga Paralemanea torulosa and new findings of the species Lemanea fluviatilis and Hildenbrandia rivularis in Serbia. The existence of all three species was recorded in the upper reaches of clean fast-flowing rivers and brooks belonging to the basin of the Danube River. Lemanea fluviatilis was found in the Dojkinačka River in Eastern Serbia, while Paralemanea torulosa was recorded in the Drina River and Hildenbrandia rivularis in the Cvetića Brook and Bioštanska Banja Brook in Western Serbia. These reports are important for conservation of the biodiversity of Serbia, since it is well known that freshwater red algae are endangered and rare species (taxa in many countries. In Serbia they are under strict protection of the law.

  16. Size-dependent resource limitation and foraging-predation risk trade-offs: growth and habitat use in young arctic char

    NARCIS (Netherlands)

    Byström, P.; Andersson, J.; Persson, L.; de Roos, A.M.

    2004-01-01

    Variation in growth and habitat use is closely connected to individual responses to habitat specific resource levels and predation risk. In three mountain lakes which differed in the density of young-of-the-year (YOY) arctic char (Salvelinus alpinus), we studied the growth, diets and habitat use of

  17. Size-dependent resource limitation and foraging-predation risk trade-offs:growth and habitat use in young artic char

    NARCIS (Netherlands)

    Bystrom, P.; Persson, L.; de Roos, A.M.; Andersson, J.A.

    2004-01-01

    Variation in growth and habitat use is closely connected to individual responses to habitat specific resource levels and predation risk. In three mountain lakes which differed in the density of young-of-the-year (YOY) arctic char (Salvelinus alpinus), we studied the growth, diets and habitat use of

  18. The prospects for Unmanned Aerial Vehicles

    OpenAIRE

    Brookes, Andrew

    2000-01-01

    In this study Andrew Brookes argues that Unmanned Aerial Vehicles (UAV) is the military fashion of the moment. Since the end of the 1990s many nations have added UAVs to their military inventories, and in 1999 half a dozen nations used UAVs over Kosovo. In the light of operational experience in Kosovo, Brookes re-evaluates the potential of this vehicle, and examines the roles, capabilities and future challenges of UAV.

  19. Physiological response of some economically important freshwater salmonids to catch-and-release fishing

    Science.gov (United States)

    Wedemeyer, G.A.; Wydoski, R.S.

    2008-01-01

    Catch-and-release fishing regulations are widely used by fishery resource managers to maintain both the quantity and quality of sport fish populations. We evaluated blood chemistry disturbances in wild brook trout Salvelinus fontinalis, brown trout Salmo trutta, cutthroat trout Oncorhynchus clarkii, and Arctic grayling Thymallus arcticus that had been hooked and played for 1-5 min in waters of the intermountain western United States. A hatchery stock of brown trout was included for comparison. To assess time needed for recovery, additional test groups were played for 5 min and then released into net-pens, where they were held for up to 72 h. The osmoregulatory and metabolic disturbances associated with catch-and-release fishing under the conditions we tested were minimal and judged to be well within normal physiological tolerance limits. In fish that were held for recovery, the blood chemistry alterations that did occur appeared to be related to stress from confinement in the net-pens. Our results confirm the results of previous studies, showing that prerelease air exposure and handling cause more physiological stress than does either hooking per se or playing time. Fishery managers must be aware of the differences in the perceptions, attitudes, and values of different societal groups, some of which feel that catch-and-release fishing should be banned because it is cruel to the animals. On the basis of brain anatomy, it seems highly unlikely that fish experience pain in the same manner as humans experience it, because fish lack a neocortex, the brain structure that enables the sensation of pain in higher vertebrates. However, independent of the neurobiological argument, our results indicate that under conditions similar to those tested, fish subjected to catch and release are neither suffering nor particularly stressed. Improved education programs about the relatively benign physiological effects of catch-and-release fishing as a fishery management practice would

  20. Earning their stripes: The potential of tiger trout and other salmonids as biological controls of forage fishes in a western reservoir

    Science.gov (United States)

    Winters, Lisa K.; Budy, Phaedra; Thiede, Gary P.

    2017-01-01

    Maintaining a balance between predator and prey populations can be an ongoing challenge for fisheries managers, especially in managing artificial ecosystems such as reservoirs. In a high-elevation Utah reservoir, the unintentional introduction of the Utah Chub Gila atraria and its subsequent population expansion prompted managers to experimentally shift from exclusively stocking Rainbow Trout Oncorhynchus mykiss to also stocking tiger trout (female Brown Trout Salmo trutta × male Brook Trout Salvelinus fontinalis) and Bonneville Cutthroat Trout O. clarkii utah (hereafter, Cutthroat Trout) as potential biological control agents. We measured a combination of diet, growth, temperature, and abundance and used bioenergetic simulations to quantify predator demand versus prey supply. Utah Chub were the predominant prey type for tiger trout, contributing up to 80% of the diet depending on the season. Utah Chub represented up to 70% of the total diet consumed by Cutthroat Trout. Although Utah Chub dominated the fish biomass in the reservoir, we still estimated abundances of 238,000 tiger trout, 214,000 Cutthroat Trout, and 55,000 Rainbow Trout. Consequently, when expanded to the population level of each predator, tiger trout and Cutthroat Trout consumed large quantities of Utah Chub on an annual basis: tiger trout consumed 508,000 kg (2,660 g/predator) of the standing prey population, and Cutthroat Trout consumed an estimated 322,000 kg (1,820 g/predator). The estimated combined consumption by Cutthroat Trout and tiger trout exceeded the estimate of Utah Chub annual production. As such, our results suggest that the high rates of piscivory exhibited by Cutthroat Trout and tiger trout in artificial lentic ecosystems are likely sufficient to effectively reduce the overall abundance of forage fishes and to prevent forage fishes from dominating fish assemblages. Collectively, this research provides the first documented findings on tiger trout ecology and performance

  1. Exploring crowded trophic niche space in a novel reservoir fish assemblage: how many predators is too many?

    Science.gov (United States)

    Winters, Lisa K.; Budy, Phaedra

    2015-01-01

    In highly managed reservoir systems, species interactions within novel fish assemblages can be difficult to predict. In high-elevation Scofield Reservoir in Utah the unintentional introduction of Utah Chub Gila atraria and subsequent population expansion prompted a shift from stocking exclusively Rainbow Trout Oncorhynchus mykiss to include tiger trout (female Brown Trout Salmo trutta × male Brook Trout Salvelinus fontinalis) and Bonneville Cutthroat Trout O. clarkii utah, which composed a novel suite of top predators and potential competitors. We examined the interspecific interactions among Scofield Reservoir piscivores using a multifaceted approach including gut analyses, stable isotopes, and gape limitation. Large Cutthroat Trout consumed 50–100% Utah Chub and tiger trout consumed 45–80%. In contrast, small and large Rainbow Trout consumed primarily invertebrate prey and exhibited significant overlap with small tiger trout, Cutthroat Trout, and Utah Chub. Large Cutthroat Trout and tiger trout occupy a top piscivore trophic niche and are more littoral, while Rainbow Trout occupy an omnivore niche space and are more pelagic. Both Cutthroat and tiger trout varied in niche space with respect to size-class, demonstrating an ontogenetic shift to piscivory at approximately 350 mm TL. Cutthroat Trout and tiger trout are capable of consuming prey up to 50% of their own size, which is larger than predicted based on their theoretical gape limit. Because it appears food resources (Utah Chub) are not limited, and performance metrics are high, competition is unlikely between Cutthroat Trout and tiger trout. In contrast, apparent survival of Rainbow Trout has recently declined significantly, potentially due to shared food resources with Utah Chub or negative behavioral interactions with other members of the community. Collectively, this research aids in understanding biotic interactions within a top-heavy and novel fish community and assists towards developing

  2. Kalispel Resident Fish Project, 2005-2006 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Olson, Jason; Andersen, Todd (Kalispel Natural Resource Department, Usk, WA)

    2006-07-01

    In 2005 the Kalispel Natural Resource Department (KNRD) monitored its current enhancement projects for bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarki lewisi). Largemouth Bass (Micropterus salmoides) enhancement projects were also monitored. Additional baseline fish population and habitat assessments were conducted, in East River and several of its tributaries.

  3. Sources and magnitude of sampling error in redd counts for bull trout

    Science.gov (United States)

    Jason B. Dunham; Bruce Rieman

    2001-01-01

    Monitoring of salmonid populations often involves annual redd counts, but the validity of this method has seldom been evaluated. We conducted redd counts of bull trout Salvelinus confluentus in two streams in northern Idaho to address four issues: (1) relationships between adult escapements and redd counts; (2) interobserver variability in redd...

  4. Amount and qualities of carotenoids in fillets of fish species fed with ...

    African Journals Online (AJOL)

    umb

    2013-03-20

    Mar 20, 2013 ... the Baltic Sea (Czeczuga and Klyszejko, 1996), Black. Sea (Czeczuga, 1973), fishing areas of the ..... (in females and eggs) through the low level of astaxan- thin and thiamine (Fitzsimons et al., .... salar L.) and Arctic charr (Salvelinus alpinus L.) specimens from an ocean ranching farm. Ocean. Hydrobiol.

  5. Simulation of groundwater conditions and streamflow depletion to evaluate water availability in a Freeport, Maine, watershed

    Science.gov (United States)

    Nielsen, Martha G.; Locke, Daniel B.

    2012-01-01

    In order to evaluate water availability in the State of Maine, the U.S. Geological Survey (USGS) and the Maine Geological Survey began a cooperative investigation to provide the first rigorous evaluation of watersheds deemed "at risk" because of the combination of instream flow requirements and proportionally large water withdrawals. The study area for this investigation includes the Harvey and Merrill Brook watersheds and the Freeport aquifer in the towns of Freeport, Pownal, and Yarmouth, Maine. A numerical groundwater- flow model was used to evaluate groundwater withdrawals, groundwater-surface-water interactions, and the effect of water-management practices on streamflow. The water budget illustrates the effect that groundwater withdrawals have on streamflow and the movement of water within the system. Streamflow measurements were made following standard USGS techniques, from May through September 2009 at one site in the Merrill Brook watershed and four sites in the Harvey Brook watershed. A record-extension technique was applied to estimate long-term monthly streamflows at each of the five sites. The conceptual model of the groundwater system consists of a deep, confined aquifer (the Freeport aquifer) in a buried valley that trends through the middle of the study area, covered by a discontinuous confining unit, and topped by a thin upper saturated zone that is a mixture of sandy units, till, and weathered clay. Harvey and Merrill Brooks flow southward through the study area, and receive groundwater discharge from the upper saturated zone and from the deep aquifer through previously unknown discontinuities in the confining unit. The Freeport aquifer gets most of its recharge from local seepage around the edges of the confining unit, the remainder is received as inflow from the north within the buried valley. Groundwater withdrawals from the Freeport aquifer in the study area were obtained from the local water utility and estimated for other categories. Overall

  6. Niche characteristics explain the reciprocal invasion success of stream salmonids in different continents

    OpenAIRE

    Korsu, Kai; Huusko, Ari; Muotka, Timo

    2007-01-01

    An ability to understand and predict invasions is elemental for controlling the detrimental effects of introduced organisms on native biota. In eastern North America, European brown trout generally dominates over, and eventually replaces, the native brook trout. We show here that in northern Europe the pattern of replacement between these two species is reversed: when transferred to North European streams, brook trout spread extensively and partially replaced the native brown trout. The effec...

  7. Models to predict suitable habitat for juvenile bull trout in Washington state

    Science.gov (United States)

    Jason B. Dunham; G. L. Chandler

    2001-01-01

    This report describes results of research conducted in 2000 to develop models of suitable habitat for juvenile bull trout (Salvelinus confluentus) in Washington State. The research is associated with a cooperative agreement (Agreement #134100H001) between U.S. Fish and Wildlife Service (USFWS) and the U.S. Forest Service, Rocky Mountain Research...

  8. Influences of temperature and environmental variables on the distribution of bull trout within streams at the southern margin of its range

    Science.gov (United States)

    J. Dunham; B. Rieman; G. Chandler

    2003-01-01

    The bull trout Salvelinus confluentus is believed to be among the most thermally sensitive species in coldwater habitats in western North America. We conducted a comprehensive field assessment of thermal habitat associations throughout the southern margin of the species' range. We developed models of thermal habitat associations using two data sets representing a...

  9. Investigations of Bull Trout (Salvelinus Confluentus), Steelhead Trout (Oncorhynchus Mykiss), and Spring Chinook Salmon (O. Tshawytscha) Interactions in Southeast Washington Streams : 1991 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Martin, Steven W.

    1992-07-01

    Bull trout (Salvelinus confluentus) are native to many tributaries of the Snake River in southeast Washington. The Washington Department of Wildlife (WDW) and the American Fisheries Society (AFS) have identified bull trout as a species of special concern which means that they may become threatened or endangered by relatively, minor disturbances to their habitat. Steelhead trout/rainbow trout (Oncorhynchus mykiss) and spring chinook salmon (O.tshawytscha) are also native to several tributaries of the Snake river in southeast Washington. These species of migratory fishes are depressed, partially due to the construction of several dams on the lower Snake river. In response to decreased run size, large hatchery program were initiated to produce juvenile steelhead and salmon to supplement repressed tributary stocks, a practice known as supplementation. There is a concern that supplementing streams with artificially high numbers of steelhead and salmon may have an impact on resident bull trout in these streams. Historically, these three species of fish existed together in large numbers, however, the amount of high-quality habitat necessary for reproduction and rearing has been severely reduced in recent years, as compared to historic amounts. The findings of the first year of a two year study aimed at identifying species interactions in southeast Washington streams are presented in this report. Data was collected to assess population dynamics; habitat utilization and preference, feeding habits, fish movement and migration, age, condition, growth, and the spawning requirements of bull trout in each of four streams. A comparison of the indices was then made between the study streams to determine if bull trout differ in the presence of the putative competitor species. Bull trout populations were highest in the Tucannon River (supplemented stream), followed by Mill Creek (unsupplemented stream). Young of the year bull trout utilized riffle and cascade habitat the most in all

  10. BIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF PRE-LARVAE OF THREE SALMONIDS SPECIES AT ONE-DAY AGE

    Directory of Open Access Journals (Sweden)

    Ye. Barylo

    2016-06-01

    Full Text Available Purpose. To study and analyze the morphometric and some biochemical parameters of pre-larval brown trout, rainbow trout and brook trout in post-embryonic period under the conditions of "Rybnyi Potik” farm in the Transcarpathian region for further use of the obtained data in scientific and practical works related to the cultivation of the juveniles of valuable salmonid species. Methodology. One-day free embryos (pre-larvae of brown trout, rainbow trout and brook trout we used as study materials. Morphometric parameters we studied by the methods of N. O. Lange, E. N. Dmitrieva. The content of total lipids was determined in accordance with Folch. in the tissuesm, which were taken for biochemical studies. Separate classes of lipids were received by thin layer chromatography. Findings. We carried out a comparative analysis of morphometric measurements and biochemical parameters of one-day pre-larval brown trout, rainbow trout and brook trout based on the obtained data. We investigated morphometric and biochemical specific features of pre-larvae in post-embryonic period and showed the species differences of morphometric measurements. Significant differences were observed between the content of lipids in the body and yolk sac of free embryos. In particular, a higher content of phospholipids and triglycerides was observed in the body of brook trout compared to brown trout. We also recorded higher contents of mono- and diacylglycerols, free cholesterol, non-etherified fatty acids (NEFA, triacylglycerols and cholesterol esters in the yolk sac of brook trout. Compared to brown trout, rainbow trout had a significant increase in mono- and diacylglycerols, free cholesterol and NEFA in both body and yolk sac as well higher levels of total lipids, triacylglycerols and cholesterol esters were registered in yolk sac. Originality. For the first time we carried out and compared the specific features of pre-larval brown trout, rainbow trout and brook trout in the

  11. Butyltin compounds in biofilm and marine organisms from the Dona Paula Bay, west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Bhosle, N.B.

    waters of California, USA (Stephenson 1991). In the C. gigas collected from the Gulf of Mexico, the TBT levels varied from 49 to 3559 ng/g (Wade, Garcia-Romero, and Brooks 1991). TBT concentrations re corded during this study suggest that TBT compounds... Indian waters Marine Environmental Research 48:123-130 Wade T L, Garcia-Romero B, and Brooks J M. 1991 Oysters as bio-monitors of butyltins in the Gulf of Mexico Marine Environmental Research 32: 233-241 ...

  12. Al Qaeda in the Indian Subcontinent (AQIS): An Al Qaeda Affiliate Case Study

    Science.gov (United States)

    2017-10-01

    september-3-2015. 7 attack and the 2008 Mumbai attacks that killed 160 people.24 Pakistan’s Inter-Services Intelligence agency (ISI) is reported to have...Modeled on Mumbai ? Why the 2008 India attack is the best way to understand Paris, Brookings Institution, November 14, 2015 https://www.brookings.edu...blog/markaz/2015/11/14/modeled-on- mumbai -why-the-2008- india-attack-is-the-best-way-to-understand-paris/ 25 Chandran, "Al-Qaeda in the Indian

  13. The Right US Strategy for Iran

    Science.gov (United States)

    2007-04-01

    King , King of Kings , King of countries containing all kinds of men, King in this great earth far and wide, son of Hystaspes, an Achaemenian , a...Highlights of the Iraq Strategy Review, January 2007, http://www.whitehouse.gov/nsc/iraq/2007/iraq-strategy011007. pdf . 13 Greg Jaffe and Neil King Jr...do if Iraq Implodes?” August 2006, Brookings Report, (Washington DC) http://www.brook.edu/views/articles/pollack/20060820. pdf . 21 Neil King Jr. and

  14. Iodine-131 in sewage sludge from a small water pollution control plant serving a thyroid cancer treatment facility.

    Science.gov (United States)

    Rose, Paula S; Swanson, R Lawrence

    2013-08-01

    Iodine-131 (half-life = 8.04 d) is the most widely used radionuclide in medicine for therapeutic purposes. It is excreted by patients and is discharged directly to sewer systems. Despite considerable dilution in waste water and the relatively short half-life of I, it is readily measured in sewage. This work presents I concentrations in sewage sludge from three water pollution control plants (WPCPs) on Long Island, NY. Iodine-131 concentrations ranged from 0.027 ± 0.002 to 148 ± 4 Bq g dry weight. The highest concentrations were measured in the Stony Brook WPCP, a relatively small plant (average flow = 6.8 × 10 L d) serving a regional thyroid cancer treatment facility in Stony Brook, NY. Preliminary radiation dose calculations suggested further evaluation of dose to treatment plant workers in the Stony Brook WPCP based on the recommendations of the Interagency Steering Committee on Radiation Standards.

  15. Distributed sensor networks

    CERN Document Server

    Rubin, Donald B; Carlin, John B; Iyengar, S Sitharama; Brooks, Richard R; University, Clemson

    2014-01-01

    An Overview, S.S. Iyengar, Ankit Tandon, and R.R. BrooksMicrosensor Applications, David ShepherdA Taxonomy of Distributed Sensor Networks, Shivakumar Sastry and S.S. IyengarContrast with Traditional Systems, R.R. BrooksDigital Signal Processing Background, Yu Hen HuImage-Processing Background Lynne Grewe and Ben ShahshahaniObject Detection and Classification, Akbar M. SayeedParameter Estimation David FriedlanderTarget Tracking with Self-Organizing Distributed Sensors R.R. Brooks, C. Griffin, D.S. Friedlander, and J.D. KochCollaborative Signal and Information Processing: AnInformation-Directed Approach Feng Zhao, Jie Liu, Juan Liu, Leonidas Guibas, and James ReichEnvironmental Effects, David C. SwansonDetecting and Counteracting Atmospheric Effects Lynne L. GreweSignal Processing and Propagation for Aeroacoustic Sensor Networks, Richard J. Kozick, Brian M. Sadler, and D. Keith WilsonDistributed Multi-Target Detection in Sensor Networks Xiaoling Wang, Hairong Qi, and Steve BeckFoundations of Data Fusion f...

  16. In vitro antituberculous activity of thymoquinone, an active principle of nigella sativa

    International Nuclear Information System (INIS)

    Randhawa, M.A.

    2011-01-01

    Background: Nigella sativa seed has been used in folk medicine against many ailments including infections. The present study was aimed to investigate activity of thymoquinone, in vitro, against clinical isolates of Mycobacterium tuberculosis. Methods: Mycobacteria obtained from patients of King Fahd Hospital of University, Alkhobar, Saudi Arabia were subcultured at 37 degree C in MGIT tubes containing Middle brook broth and OADC growth supplement and growth detected by BACTEC Micro MGIT fluoro meter on day 10. Mycobacteria were then inoculated in MGIT tubes containing thymoquinone 2.5, 5, 10, 20, 40, 80 micro g/ml, streptomycin 1.25 micro g/ml or controls in Middle brook broth plus supplement, incubated at 37 degree C for 14 days and read daily for fluorescence. In addition, isolates were inoculated in culture tubes containing Middle brook agar (plus supplement) in presence of thymoquinone 2.5, 5, 10, 20, 40, 80 micro g/ml, streptomycin 1.25 micro g/ml or controls, and incubated at 37 degree C for 4 weeks. Results: In Middle brook broth, fluorescence test for tuberculosis was negative with thymoquinone 20, 40 and 80 micro g/ml and streptomycin 1.25 micro g/ml up to day 14. With controls, thymoquinone 2.5, 5 and 10 micro g/ml fluorescence was detectable from day 10 to 14. In Middle brook agar, there was no visible growth of tubercle bacillus with thymoquinone 20, 40 and 80 micro g/ml and streptomycin 1.25 micro g/ml, however, with controls, thymoquinone 2.5 and 5 micro g/ml abundant and with 10 micro g/ml few colonies were observed. Conclusions: Thymoquinone possesses activity against M. tuberculosis with MIC of 20 micro g/ml and has potential for further investigation. Our study confirms the benefit of N. sativa in native medicine against chest infection. (author)

  17. Fish Health Study Ashtabula River Natural Resource Damage Assessment

    Science.gov (United States)

    Blazer, V.S.; Iwanowicz, L.R.; Baumann, P.C.

    2006-01-01

    INTRODUCTION The Ashtabula River is located in northeast Ohio, flowing into Lake Erie at Ashtabula, Ohio. Tributaries include Fields Brook, Hubbard Run, Strong Brook, and Ashtabula Creek. The bottom sediments, bank soils and biota of Fields Brook have been severely contaminated by unregulated discharges of hazardous substances. Hazardous substances have migrated downstream from Fields Brook to the Ashtabula River and Harbor, contaminating bottom sediments, fish and wildlife. There are presently more than 1,000,000 cubic yards of contaminated sediment in the Ashtabula River and Harbor, much of which originated from Fields Brook. Contaminants include polychlorinated biphenyls (PCBs), chlorinated benzenes, chlorinated ethenes, hexachlorobutadiene, polyaromatic hydrocarbons (PAHs), other organic chemicals, heavy metals and low level radionuclides. A Preassessment Screen, using existing data, was completed for the Ashtabula River and Harbor on May 18, 2001. Among the findings was that the fish community at Ashtabula contained approximately 45 percent fewer species and 52 percent fewer individuals than the Ohio EPA designated reference area, Conneaut Creek. The Ashtabula River and Conneaut Creek are similar in many respects, with the exception of the presence of contamination at Ashtabula. The difference in the fish communities between the two sites is believed to be at least partially a result of the hazardous substance contamination at Ashtabula. In order to investigate this matter further, the Trustees elected to conduct a study of the status and health of the aquatic biological communities of the Ashtabula River and Conneaut Creek in 2002-2004. The following document contains brief method descriptions (more detail available in attached Appendix A) and a summary of the data used to evaluate the health status of brown bullheads (Ameiurus nebulosus) and largemouth bass (Micropterus salmoides) collected from the above sites.

  18. Development of bull trout sampling protocols

    Science.gov (United States)

    R. F. Thurow; J. T. Peterson; J. W. Guzevich

    2001-01-01

    This report describes results of research conducted in Washington in 2000 through Interagency Agreement #134100H002 between the U.S. Fish and Wildlife Service (USFWS) and the U.S. Forest Service Rocky Mountain Research Station (RMRS). The purpose of this agreement is to develop a bull trout (Salvelinus confluentus) sampling protocol by integrating...

  19. Improved Algorithms Speed It Up for Codes

    International Nuclear Information System (INIS)

    Hazi, A

    2005-01-01

    Huge computers, huge codes, complex problems to solve. The longer it takes to run a code, the more it costs. One way to speed things up and save time and money is through hardware improvements--faster processors, different system designs, bigger computers. But another side of supercomputing can reap savings in time and speed: software improvements to make codes--particularly the mathematical algorithms that form them--run faster and more efficiently. Speed up math? Is that really possible? According to Livermore physicist Eugene Brooks, the answer is a resounding yes. ''Sure, you get great speed-ups by improving hardware,'' says Brooks, the deputy leader for Computational Physics in N Division, which is part of Livermore's Physics and Advanced Technologies (PAT) Directorate. ''But the real bonus comes on the software side, where improvements in software can lead to orders of magnitude improvement in run times.'' Brooks knows whereof he speaks. Working with Laboratory physicist Abraham Szoeke and others, he has been instrumental in devising ways to shrink the running time of what has, historically, been a tough computational nut to crack: radiation transport codes based on the statistical or Monte Carlo method of calculation. And Brooks is not the only one. Others around the Laboratory, including physicists Andrew Williamson, Randolph Hood, and Jeff Grossman, have come up with innovative ways to speed up Monte Carlo calculations using pure mathematics

  20. BIOLOGICAL WATER QUALITY ASSESSMENT OF THE WHITECLAWED CRAYFISH HABITAT BASED ON MACROINVERTEBRATE COMMUNITIES: USEFULNESS FOR ITS CONSERVATION

    Directory of Open Access Journals (Sweden)

    GRANDJEAN F.

    2003-04-01

    Full Text Available A survey of the macroinvertebrates of three brooks harbouring the white-clawed crayfish was conducted in Haute-Vienne department (France. Its aim was to increase our understanding of these ecosystems to help the conservation of A. pallipes. These brooks run through pastoral areas with well-developed riparian vegetation, which offers an important shade. Water temperature, dissolved oxygen concentration, pH, and conductivity fell within the ranges found for this species. A total of 34, 31, 29 taxa and 1 502, 1 364, 2 707 individuals of macrobenthos were collected in Holme, Besque and Bellecombe streams, respectively. Results showed good to very good water quality with IBGN scores ranging from 15 (Bellecombe to 17 (Holme and Besque, reflecting a limited impact of the anthropogenic disturbances. Taxa diversity were high for Holme and Besque with Shannon index around 3.2, translating a great heterogeneity of habitat and an equilibrated faunal community. Bellecombe showed a limited diversity with Shannon index of 1.42, resulting from the presence of numberous Chironomidae. This brook suffers probably weak organic pollution which could be related to the low water flow observed during the sampling. The similarity test according to Jaccard index showed high percentage of common taxa among ETP (Ephemeroptera-Trichoptera-Plecoptera between all sites. The high similarity of benthic macroinvertebrate communities could be an useful criteria to identify brooks for restocking purpose.