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Sample records for saintpaulia ionantha saccharum

  1. Gray Mold on Saintpaulia ionantha Caused by Botrytis cinerea in Korea

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    Hyung-Moo Kim

    2011-04-01

    Full Text Available Gray mold caused by Botrytis cinerea occurred on Saintpaulia ionantha in flower shop of the Jeonju city in Korea. Typical symptoms with brown water-soaked and rotting lesions were appeared on the flowers, leaves and petiole of infected plants. Many conidia spores appeared on the lesions under humid conditions. Colonies were grayish brown and sclerotial formation on potato dextrose agar. Conidia were one celled, mostly ellipsoidal or ovoid in shape, and were colorless to pale brown in color. The conidia were 7~14×5~9 μm in size. Based on pathogenicity and morphological characteristics of the isolated fungus, the causal fungus was identified as B. cinerea Persoon: Fries. Gray mold of S. ionantha was proposed to the name of this disease.

  2. Micropropagation of African violet (Saintpaulia ionantha Wendl.).

    Science.gov (United States)

    Shukla, Mukund; Sullivan, J Alan; Jain, Shri Mohan; Murch, Susan J; Saxena, Praveen K

    2013-01-01

    Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of explants used, physiological state of the mother plant, plant growth regulators in the culture medium, and growth conditions. Development of cost-effective protocols with a high rate of multiplication is a crucial requirement for commercial application of micropropagation. The current chapter describes an optimized protocol for micropropagation of African violets using leaf explants obtained from in vitro grown plants. In this process, plant regeneration occurs via both somatic embryogenesis and shoot organogenesis simultaneously in the explants induced with the growth regulator thidiazuron (TDZ; N-phenyl-N'-1,2,3-thidiazol-5-ylurea). The protocol is simple, rapid, and efficient for large-scale propagation of African violet and the dual routes of regeneration allow for multiple applications of the technology from simple clonal propagation to induction or selection of variants to the production of synthetic seeds.

  3. The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl.

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    Marek Gaj

    2014-01-01

    Full Text Available The leaf-explants of Saintpaulia ionantha Wendl. var.'miniature' were treated by different doses of MNH and cultured on shoot regeneration medium. A strong toxic effect of some MNH doses on explant survival during the first two subcultures was noticed. The explants surviving treatment regenerated shoots with the efficiency comparable to the control. The high number of shoots regenerated from mutagenised leaves showed chlorophyll chimerism (so-called variegated forms. The use of 5 mM MNH for 1.5 or 2 h was found very effective, as 100% of survived explants regenerated variegated shoots. Besides hundreds of variegated forms also leaf-shape and flower-colour variants were observed in MNH-treated culture. Somaclonal variation was not observed in the control culture. The results indicate the great efficiency of in vitro applied MNH for in-duction of morphological variants of Saintpaulia, and especially variegated forms.

  4. Effects of chronic gamma irradiation on adventitious plantlet formation of Saintpaulia ionantha (African violet) detached leaves

    International Nuclear Information System (INIS)

    Arunee Wongpiyasatid; Peeranuch Jompuk; Katarat Chusreeaeom; Thanya Taychasinpitak

    2007-01-01

    Formation of adventitious plantlets on unrootedly detached leaves of two African violet (Saintpaulia ionantha) cultivars, pink and violet flowers, chronically gamma-irradiated in gamma room at The Gamma Irradiation Service and Nuclear Technology Research Center, Kasetsart University was compared. Detached leaves were immediately planted after detachment in plastic trays containing peat moss, 18 leaves per treatment with 3 replications. Three dose rates (rad/h) with 3 doses (rad)/dose rate, were applied to the irradiated samples while the controls were placed outside the gamma room. Three months after irradiation, the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf were recorded. After that, the young plantlets were transferred to the new pots for further observation on plant growth and mutation characters. The results revealed that the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf varied slightly with radiation doses but were not significantly different at different dose rates. Radiosensitivity was noticed to be higher in pink flower cultivar than the violet one. M 1 V 1 plantlets will be followed up for growth and mutation character observations

  5. 非洲紫罗兰组织培养体系的建立%Establishing the Tissue Culture System for Saintpaulia Ionantha

    Institute of Scientific and Technical Information of China (English)

    尹立辉; 武术杰; 刘亚亮; 王雪松

    2016-01-01

    以紫色白边单瓣花的非洲紫罗兰叶片为试材进行了组织培养技术的研究。结果表明:以叶片为外植体进行组织培养时,愈伤组织诱导最适培养基为MS+0.2 mg/L NAA+0.5 mg/L6-BA,培养3周左右诱导率达100%;继代增殖时最适培养基为MS+0.02 mg/L NAA+0.1 mg/L 6-BA;无需生根培养,直接瓶外炼苗,栽植于以m (草炭土)∶m(珍珠岩)=1∶1的基质中生长最好,成活率可达95%以上。%We studied the tissue culture technology of Saintpaulia Ionantha with the purple petals of white edge and single flower . The tissue culture was conducted with the leaves as explants .The inducing medium for callus was MS+0.2 mg/L NAA+0.5 mg/L 6-BA.The induction rate after three weeks was100 %.The most suitable medium for subculture was MS+0.02 mg/L NAA+0.1 mg/L 6-BA.The outk-tube rooting rate were relatively better without taking root in the bottle .The best matrix for planting effect was peat soil and perlite with the mixed proportion of 1∶1 and the survival rate of >95%.

  6. Use in plant breeding of acute, chronic or fractionated doses of X-rays or fast neutrons as illustrated with leaves of Saintpaulia

    NARCIS (Netherlands)

    Broertjes, C.

    1971-01-01


    The parameters used were survival of irradiated leaves of S. ionantha , production of adventitious plantlets at the base of the petiole and mutation frequency.

    The differences between unfiltered X-rays from a 250/25 deep therapy apparatus and fast neutrons

  7. Foliar trichome- and aquaporin-aided water uptake in a drought-resistant epiphyte Tillandsia ionantha Planchon.

    Science.gov (United States)

    Ohrui, T; Nobira, H; Sakata, Y; Taji, T; Yamamoto, C; Nishida, K; Yamakawa, T; Sasuga, Y; Yaguchi, Y; Takenaga, H; Tanaka, Shigeo

    2007-12-01

    The atmospheric epiphyte Tillandsia ionantha is capable of surviving drought stress for 6 months or more without any exogenous water supply via an as of yet to be determined mechanism. When plants were soaked in water for 3 h, leaves absorbed a remarkably large amount of water (30-40% on the basis of fresh weight), exhibiting a bimodal absorption pattern. Radiolabeled water was taken up by the leaves by capillary action of the epidermal trichomes within 1 min (phase 1) and then transported intracellularly to leaf tissues over 3 h (phase 2). The removal of epidermal trichome wings from leaves as well as rinsing leaves with water significantly lowered the extracellular accumulation of water on leaf surfaces. The intracellular transport of water was inhibited by mercuric chloride, implicating the involvement of a water channel aquaporin in second-phase water absorption. Four cDNA clones (TiPIP1a, TiPIP1b, TiPIP1c, and TiPIP2a) homologous to PIP family aquaporins were isolated from the leaves, and RT-PCR showed that soaking plants in water stimulated the expression of TiPIP2a mRNA, suggesting the reinforcement in ability to rapidly absorb a large amount of water. The expression of TiPIP2a complementary RNA in Xenopus oocytes enhanced permeability, and treatment with inhibitors suggested that the water channel activity of TiPIP2a protein was regulated by phosphorylation. Thus, the high water uptake capability of T. ionantha leaves surviving drought is attributable to a bimodal trichome- and aquaporin-aided water uptake system based on rapid physical collection of water and subsequent, sustained chemical absorption.

  8. The chemical constituents from leaves of Acer saccharum.

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    Zhang, Yu; Zhao, Hong

    2009-03-01

    To study the chemical constituents of leaves of Acer saccharum. The leaves of Acer saccharum were extracted by ethanol. The concentrated material was partitioned by petroleum ether, ethyl acetate, and n-butanol. After extracted by ethyl acetate, the extract was isolated and purified by silica gel column chromatography and recrystallization. The compound structures were identified on the basis of spectral data and chemical methods. Seven compounds were isolated from the leaves of Acer saccharum. Their compound structures were identified as 3-keton-ursane,3beta-hydroxy-12-olean-12-en and 5-en-7-hydroxy-sitosterol. All the three compounds identified are isolated from this genus for the first time.

  9. Bioacetylation of alcohols catalysed by Saccharum officinarum; Bioacetilacao de alcoois catalisada por Saccharum officinarum

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    Assuncao, Joao Carlos C.; Lemos, Telma Leda G.; Monte, Francisco Jose Q. [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica], e-mail: fmonte@dqoi.ufc.br

    2009-07-01

    Lipase-catalysed esterifications of alcohols using immobilized enzyme system from sugar cane (Saccharum officinarum) as biocatalyst afforded the corresponding esters in considerable yields (68-93%). Under optimized conditions, the material was utilized for reactions of acetylation with several advantage. It also investigated the possibility of reuse of immobilized enzymes of S. officinarum as biocatalyst under optimal reaction conditions. (author)

  10. Second generation bioethanol production from Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.

    Science.gov (United States)

    Danilo Scordia; Salvatore L. Consentino; Thomas W. Jeffries

    2010-01-01

    Saccharum (Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.), is a rapidly growing, wide ranging high-yield perennial, suitable for second generation bioethanol production. This study evaluated oxalic acid as a pretreatment for bioconversion. Overall sugar yields, sugar degradation products, enzymatic glucan hydrolysis and ethanol production were studied as...

  11. Eco-physiology of Acer saccharum trees on glade-like sites in central Missouri

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    Eric J. Rhodenbaugh; Stephen G. Pallardy

    1993-01-01

    Although sugar maple (Acer saccharum Marsh.) is not considered drought tolerant, it is common on xeric limestone glade-like sites in central Missouri. Acer saccharum on such sites may be a drought-tolerant ecotype or may have access to deep water supply through bedrock cracks. We investigated these possibilities during the 1990...

  12. The regeneration of epidermal cells of Saintpaulia leaves as a new plant-tissue system for cellular radiation biology

    International Nuclear Information System (INIS)

    Engels, F.M.; Laan, F.M. van der; Leenhouts, H.P.; Chadwick, K.H.

    1980-01-01

    investigation of the nucleus of epidermal cells of the petioles of Saintpaulia leaves by cytofluorimetry revealed that all cells are in a non-cycling pre DNA synthesis phase. Cultivation of dissected leaves results in a synchronous regeneration process of a defined number of cells. Five days after onset of cultivation the cells reach the first mitosis. The nuclear development during the regeneration process is described. Irradiation of the leaves results in a directly visible inhibition of this regenerating capability which is used to quantify cell survival in a tissue. The data show that the radiation response has a similar shape to that of the survival of single cells in culture. This response can be observed before the first mitosis of the cells and its application as a new plant tissue system for cellular radiation research is discussed. (author)

  13. Biohydrolysis of Saccharum spontaneum for cellulase production by ...

    African Journals Online (AJOL)

    Saccharum spontaneum, a wasteland weed, is utilized for cellulase production by Aspergillus terreus in solid state fermentation. S. spontaneum served as good carbon source and solid support. Various process parameters including optimal nitrogen source, initial moisture level, incubation time, initial pH, incubation ...

  14. Histological relationship of Phytobia setosa to Acer saccharum

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    Robert Gregory; William. Wallner

    1979-01-01

    The maple cambium miner, Phytobia setosa (Loew), attacks Acer spp., producing ray flecks which result in degrade in face veneer and furniture wood. Samples from infested sugar maple, Acer saccharum Marsh, trees demonstrated that while mines passed close to the vascular cambium the initial cells were not...

  15. Identification of intergeneric hybrids between Saccharum spp. and ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-02-27

    Feb 27, 2009 ... The reason was that there might be a certain maternal-specific marker just on one homologous chromosome, while the other one without this marker, among the highly heterozygous genome of Saccharum spp., and that the recombination of genes and chromosome happened in the period of meiosis might ...

  16. Monitoring the Health of Sugar Maple, "Acer Saccharum"

    Science.gov (United States)

    Carlson, Martha

    2013-01-01

    The sugar maple, "Acer saccharum," is projected to decline and die in 88 to 100 percent of its current range in the United States. An iconic symbol of the northeastern temperate forest and a dominant species in this forest, the sugar maple is identified as the most sensitive tree in its ecosystem to rising temperatures and a warming…

  17. Do Interspecific Differences in Sapling Growth Traits Contribute to the Co-dominance of Acer saccharum and Fagus grandifolia?

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    Takahashi, Koichi; Lechowicz, Martin J.

    2008-01-01

    Background and Aims Acer saccharum and Fagus grandifolia are among the most dominant late-successional tree species in North America. The influence of sapling growth responses to canopy gaps on the co-dominance of the two species in an old-growth forest in southern Quebec, Canada was examined. Two predictions were evaluated: (a) F. grandifolia is more shade tolerant than A. saccharum due to greater sapling leaf area and net production per sapling in closed-canopy conditions; and (b) the height growth rate of A. saccharum in canopy gaps is greater than that of F. grandifolia due to increased net production per sapling. Methods Sapling crown allometry, net production and height growth rates were compared between and within the two species in closed canopy vs. canopy gaps. Standardized major axis regression was used to analyse differences in crown allometry. Key Results F. grandifolia had greater crown projection, sapling leaf area and net production rate per sapling than A. saccharum in closed-canopy conditions. In response to canopy gaps, net production per sapling increased to the same degree in both species. The net production per sapling of F. grandifolia thus was much greater than that of A. saccharum in both canopy gap and closed-canopy conditions. The height growth rate of both species increased in canopy gaps, but the degree of increase was greater in F. grandifolia than in A. saccharum. Conclusions F. grandifolia regenerated more successfully than A. saccharum in both closed-canopy conditions and canopy gaps, which indicates that the co-dominance of the two species cannot be maintained simply by interspecific differences in shade tolerance and growth in gaps. Previous research showed that although Fagus and Acer shared dominance at this site, their relative dominance shifted with edaphic conditions. This suggests that the widespread co-dominance of the two species in eastern North American forests is maintained by the joint influence of canopy disturbance

  18. Assessing the Likelihood of Gene Flow From Sugarcane (Saccharum Hybrids to Wild Relatives in South Africa

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    Sandy J. Snyman

    2018-06-01

    Full Text Available Pre-commercialization studies on environmental biosafety of genetically modified (GM crops are necessary to evaluate the potential for sexual hybridization with related plant species that occur in the release area. The aim of the study was a preliminary assessment of factors that may contribute to gene flow from sugarcane (Saccharum hybrids to indigenous relatives in the sugarcane production regions of Mpumalanga and KwaZulu-Natal provinces, South Africa. In the first instance, an assessment of Saccharum wild relatives was conducted based on existing phylogenies and literature surveys. The prevalence, spatial overlap, proximity, distribution potential, and flowering times of wild relatives in sugarcane production regions based on the above, and on herbaria records and field surveys were conducted for Imperata, Sorghum, Cleistachne, and Miscanthidium species. Eleven species were selected for spatial analyses based on their presence within the sugarcane cultivation region: four species in the Saccharinae and seven in the Sorghinae. Secondly, fragments of the nuclear internal transcribed spacer (ITS regions of the 5.8s ribosomal gene and two chloroplast genes, ribulose-bisphosphate carboxylase (rbcL, and maturase K (matK were sequenced or assembled from short read data to confirm relatedness between Saccharum hybrids and its wild relatives. Phylogenetic analyses of the ITS cassette showed that the closest wild relative species to commercial sugarcane were Miscanthidium capense, Miscanthidium junceum, and Narenga porphyrocoma. Sorghum was found to be more distantly related to Saccharum than previously described. Based on the phylogeny described in our study, the only species to highlight in terms of evolutionary divergence times from Saccharum are those within the genus Miscanthidium, most especially M. capense, and M. junceum which are only 3 million years divergent from Saccharum. Field assessment of pollen viability of 13 commercial sugarcane

  19. Assessing the Likelihood of Gene Flow From Sugarcane (Saccharum Hybrids) to Wild Relatives in South Africa

    Science.gov (United States)

    Snyman, Sandy J.; Komape, Dennis M.; Khanyi, Hlobisile; van den Berg, Johnnie; Cilliers, Dirk; Lloyd Evans, Dyfed; Barnard, Sandra; Siebert, Stefan J.

    2018-01-01

    Pre-commercialization studies on environmental biosafety of genetically modified (GM) crops are necessary to evaluate the potential for sexual hybridization with related plant species that occur in the release area. The aim of the study was a preliminary assessment of factors that may contribute to gene flow from sugarcane (Saccharum hybrids) to indigenous relatives in the sugarcane production regions of Mpumalanga and KwaZulu-Natal provinces, South Africa. In the first instance, an assessment of Saccharum wild relatives was conducted based on existing phylogenies and literature surveys. The prevalence, spatial overlap, proximity, distribution potential, and flowering times of wild relatives in sugarcane production regions based on the above, and on herbaria records and field surveys were conducted for Imperata, Sorghum, Cleistachne, and Miscanthidium species. Eleven species were selected for spatial analyses based on their presence within the sugarcane cultivation region: four species in the Saccharinae and seven in the Sorghinae. Secondly, fragments of the nuclear internal transcribed spacer (ITS) regions of the 5.8s ribosomal gene and two chloroplast genes, ribulose-bisphosphate carboxylase (rbcL), and maturase K (matK) were sequenced or assembled from short read data to confirm relatedness between Saccharum hybrids and its wild relatives. Phylogenetic analyses of the ITS cassette showed that the closest wild relative species to commercial sugarcane were Miscanthidium capense, Miscanthidium junceum, and Narenga porphyrocoma. Sorghum was found to be more distantly related to Saccharum than previously described. Based on the phylogeny described in our study, the only species to highlight in terms of evolutionary divergence times from Saccharum are those within the genus Miscanthidium, most especially M. capense, and M. junceum which are only 3 million years divergent from Saccharum. Field assessment of pollen viability of 13 commercial sugarcane cultivars using

  20. Phenolic glycosides from sugar maple (Acer saccharum) bark.

    Science.gov (United States)

    Yuan, Tao; Wan, Chunpeng; González-Sarrías, Antonio; Kandhi, Vamsikrishna; Cech, Nadja B; Seeram, Navindra P

    2011-11-28

    Four new phenolic glycosides, saccharumosides A-D (1-4), along with eight known phenolic glycosides, were isolated from the bark of sugar maple (Acer saccharum). The structures of 1-4 were elucidated on the basis of spectroscopic data analysis. All compounds isolated were evaluated for cytotoxicity effects against human colon tumorigenic (HCT-116 and Caco-2) and nontumorigenic (CCD-18Co) cell lines.

  1. Comparison of the growth and biomass production of Miscanthus sinensis, Miscanthus floridulus and Saccharum arundinaceum

    Energy Technology Data Exchange (ETDEWEB)

    Feng, X.; He, Y.; Fang, J.; Fang, Z.; Jiang, B.; Brancourt-Hulmel, M.; Zheng, B.; Jiang, D.

    2015-07-01

    Miscanthus and Saccharum are considered excellent candidates for bioenergy feedstock production. A field experiment was conducted in Zhejiang province of China to characterize the phenotypic differences in three species, two of Miscanthus (M. sinensis and M. floridulus) and one of Saccharum (S. arundinaceum), each with two accessions collected from China. Agronomical traits, including plant height, culm number, tuft diameter and culm diameter, were monitored monthly for the first 3 years of growth. For each year of trail, flowering time was observed and biomass yield was harvested. M. floridulus produced a superior biomass yield with increasing plant age associated with higher yields (4.18, 24.16 and 29.01 t dry matter/hain November of years one to three, respectively). Higher culm diameter, plant height and tuft diameter values were observed for M. floridulus when compared to the other species. Biomass yield was positively correlated to tuft diameter, culm diameter, culm number and negatively to flowering time, but it showed no correlation with plant height. Tuft diameter and culm diameter could be suitable indicators in the selection of accessions for crop yield at the yield-building phase. Studies of the primary colonizers of Miscanthus and Saccharum in their original location may be of interest from the perspective of bioenergy germplasm resource collection. (Author)

  2. Use of 15N-isotope dilution for quantification of nitrogen fixation in Saccharum SPP

    International Nuclear Information System (INIS)

    Singh, M.; Singh, G.B.; Joshi, B.B.

    1994-01-01

    The objectives of present study were to quantify and compare the amount of nitrogen fixed by diazotrophes associated with roots of Saccharum spontaneum, S. barberi and S. sinense using Sclerotachya fusca as a non-fixing control. 5 refs

  3. Characterizing water use strategies of Acer saccharum, Liriodendron tulipifera, and Quercus spp. during a severe drought

    Science.gov (United States)

    Yi, K.; Novick, K. A.; Dragoni, D.; Moore, W.; Roman, D. T.

    2014-12-01

    In many areas, drought is expected to occur more frequently and intensely in the future due to climate change; however, drought effects on ecosystem-scale fluxes in diverse forests will reflect the diversity of water use strategies among the dominant tree species. For three years (2011-2013) that included a severe drought event (in 2012), we measured the sap flow densities along the sapwood profiles (four radial depths: 1, 2, 3, 4 cm) in Acer saccharum, Liriodendron tulipifera, and Quercus spp. using the compensation heat pulse technique at the Morgan-Monroe State Forest (Indiana, USA). Sap flow velocity varies along the radial profile of the stem, and thus characterizing its pattern is important for estimating whole tree sap flow, and for characterizing the extent to which water stress alters the radial pattern of flow. We also focused on the nocturnal sap flow, which may be used to replenish stored water depleted during the daytime, in order to assess the extent to which the three species rely on hydraulic capacitance to cope with water stress. Sap flow densities along the sapwood profile of all three species tended to increase toward the cambium under moderate climate, while the tendency was reversed under severe drought. This shift may indicate greater reliance on stored water in the inner sapwood or cavitation of outer sapwood during the drought. It was also noticeable that Quercus spp. showed lower maximum sap flow density and narrower range (1.5 - 4.6 cm h-1) than other species (A. saccharum: 1.0 - 20.8 cm h-1, L. tulipifera: < 0.1 - 45.2 cm h-1) during 3 years of measurements. In addition, nocturnal/diurnal ratios of volumetric sap flows were significantly higher in the drought year for A. saccharum (0.140.01 in 2011 and 0.200.01 in 2013 vs. 0.290.01 in 2012) and L. tulipifera (0.140.00 in 2011 and 0.090.01 in 2013 vs. 0.300.01 in 2012), while Quercus spp. didn't show a significant difference between moderate and drought years. This may be due to the

  4. Seasonal patterns of reserve and soluble carbohydrates in mature sugar maple (Acer saccharum)

    Science.gov (United States)

    B.L. Wong; K.L. Baggett; A.H. Rye

    2003-01-01

    Sugar maple (Acer saccharum Marsh.) trees exhibit seasonal patterns of production, accumulation, and utilization of nonstructural carbohydrates that are closely correlated with phenological events and (or) physiological processes. The simultaneous seasonal patterns of both reserve and soluble carbohydrates in the leaves, twigs, branches, and trunks of healthy mature...

  5. Molecular identification and genetic diversity analysis of Chinese sugarcane (Saccharum spp. hybrids) varieties using SSR markers

    Science.gov (United States)

    Sugarcane (Saccharum spp. hybrids) is an important sugar and renewable bioenergy crop. However, its complex aneupolyploidy genome and vegetative mode of propagation often cause difficulty in selection and some variety identity issues in a breeding program. Therefore, the present study was set up to ...

  6. Spectroscopic characterization of D-003 obtained from the sugar cane (Saccharum officinarum L.) wax

    International Nuclear Information System (INIS)

    Marrero Delange, David; Cora Medina, Miriam; Laguna Granja, Abilio; Gonzalez Canavaciolo Victor L

    2013-01-01

    D-003, an active pharmaceutical ingredient (API) purified from sugar cane (Saccharum officinarum L.) wax with cholesterol-lowering and antioxidant effects, is composed of a mixture of free saturated very long chain fatty acids (VLCFAs), each within specific relative concentration ranges as determined by the gas chromatography (GC). However, the spectroscopic characterization of D-003 had not been previously reported

  7. Spectroscopic characterization of Simultaneous determination of Albendazol from the sugar cane (Saccharum officinarum L.) wax

    International Nuclear Information System (INIS)

    Marrero Delange, David; Cora Medina, Miriam; Laguna Granja, Abilio; Gonzalez Canavaciolo, Victor L

    2013-01-01

    D-003, an active pharmaceutical ingredient (API) purified from sugar cane (Saccharum officinarum L.) wax with cholesterol-lowering and antioxidant effects, is composed of a mixture of free saturated very long chain fatty acids (VLCFAs), each within specific relative concentration ranges as determined by the gas chromatography (GC). However, the spectroscopic characterization of D-003 had not been previously reported

  8. Laser-induced breakdown spectroscopy for the study of the pattern of silicon deposition in leaves of saccharum species

    NARCIS (Netherlands)

    Tripathi, D.K.; Kumar, R.; Chauhan, D.K.; Rai, A.K.; Bicanic, D.D.

    2011-01-01

    The spatial distribution pattern of silicon in the leaves of three species of Saccharum has been demonstrated by means of laser induced breakdown spectroscopy (LIBS). The in-situ point detection capability of LIBS was used to determine different elements in leaf samples. The concentrations of

  9. Application of molecular markers in sugarcane germplasm innovation and breeding: new germplasm with cytoplasm from Saccharum spontaneum

    Science.gov (United States)

    All current sugarcane cultivars (Saccharum hybrids spp.) are interspeci'c hybrids of S. of'cinarum, S. robustum and S. spontaneum that bear the same cytoplasm of S. of'cinarum. Until the end of 20th century, S. spontaneum was exclusively used as male parents to confer such traits as vigor, ratoonabi...

  10. Constructing high-density genetic maps for polyploid sugarcane (Saccharum spp.) and identifying quantitative trait loci controlling brown rust resistance

    Science.gov (United States)

    Sugarcane (Saccharum spp.) is an important economic crop for producing edible sugar and bioethanol. Brown rust had long been a major disease impacting sugarcane production world widely. Resistance resource and markers linked to the resistance are valuable tools for disease resistance improvement. An...

  11. Liming Influences Growth and Nutrient Balances in Sugar Maple (Acer saccharum) Seedlings on an Acidic Forest Soil

    Science.gov (United States)

    Dudley J. Raynal

    1998-01-01

    Forests in the northeastern US have been limed to mitigate soil acidification and the acidity of surface waters and to improve soil base cation status. Much of the area considered for liming is within the range of sugar maple (Acer saccharum), but there is a poor understanding of how liming influences growth and nutrient balance of this species on...

  12. Evaluation of the juice brix of wild sugarcanes (Saccharum spontaneum indigenous to Japan

    Directory of Open Access Journals (Sweden)

    Takeo Sakaigaichi

    2016-04-01

    Full Text Available Modern sugarcane cultivars are derived from the interspecific crossing between Saccharum officinarum and wild sugarcane, Saccharum spontaneum. The introgression of valuable characteristics from wild sugarcane is recognized as extremely important, but this process typically requires long-term effort over multiple generations of backcrosses owing to the low sugar content of the initial interspecific hybrids. In this study, we aimed to identify Japanese wild sugarcanes with high juice brix in order to promote effective interspecific crossing of sugarcane. Sixty-four accessions from the Nansei Islands and 70 accessions from the Honshu were evaluated for juice brix. Wild sugarcanes with high juice brix were demonstrated to exist among wild sugarcanes indigenous to the Honshu. A significant difference was observed between the median juice brix values of wild sugarcanes of the Nansei Islands and those of the Honshu. The relationship between juice brix and stem traits was then examined in 20 wild sugarcanes, 10 each from the Nansei Islands and the Honshu. The reproducibility of juice brix value in both experiments was confirmed. In contrast to juice brix, stem traits, such as length, diameter, and volume, were typically smaller in wild sugarcanes from the Honshu. Moreover, a negative correlation was observed between the index of stem volume and juice brix. In this study, we identified outstanding wild sugarcanes with high juice brix. Using germplasms from the identified wild sugarcanes in interspecific crossing could contribute to the increases in both yield and sugar content.

  13. HONGOS MICORRÍCICO-ARBUSCULARES EN LA PRODUCCIÓN DE VIOLETA AFRICANA EN UN SISTEMA DE MANEJO TRADICIONAL

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    Ramón Zulueta-Rodríguez

    2013-01-01

    Full Text Available Se determinó el efecto de la inoculación de violeta africana ( Saintpaulia ionantha Wendl. con hongos micorrícico-arbusculares (HMA en un vivero comercial bajo el sistema de cultivo del productor. Se probaron dos presentaciones del inóculo, raíces frescas (IT e inoculante encapsulado en perlas de alginato de calcio (IE. Los tratamientos establecidos fueron plantas inoculadas con cada uno de los inóculos (IT e IE, plantas fertilizadas sin inocular (F, plantas inoculadas + fertilizante (IT+F e IE+F y plantas testigo (T. Las variables evaluadas 90 y 180 días después de la inoculación (DDI fueron área foliar, número de hojas, de botones florales y de flores, así como la longitud de raíz colonizada, peso seco de pecíolos, hojas y raíces al final del experimento (180 DDI. El análisis estadístico indicó diferencias altamente significativas entre los tratamientos a los 90 DDI para área foliar, número de botones florales y de flores (ANOVA, P ≤ 0.01, respuesta que prevaleció hasta los 180 DDI con respecto a las plantas testigo (ANOVA, P ≤ 0.01. En general, la interacción de los HMA con el fertilizante promovió una floración prematura, lo cual indica que el uso de estos microorganismos puede considerarse una alterna - tiva biotecnológica factible de incorporar en estos sistemas de producción.

  14. Root extracts of Bracchiaria humidicola andSaccharum spontaneum to increase N use by sugarcane

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    Oriel Tiago Kölln

    2016-02-01

    Full Text Available ABSTRACT Retaining the mineral N in the form of NH4+ in the soil for a lengthy period is desirable for reducing losses. Furthermore, there is evidence that sugarcane prefers NH4+-N in place of NO3−-N. This study aimed firstly, to evaluate the potential of root extracts of Bracchiaria humidicola andSaccharum spontaneum, in contrast with the DCD (Dicyandiamide inhibitor, to increase absorption of N by plants fertilized with ammonium sulfate, and secondly, to quantify the emission of N2O fluxes with the use of this inhibitor. The experiment was developed in a glasshouse in an entirely randomized design where four treatments were applied: AS ammonium sulfate (control; AS+DCD ammonium sulfate associated with dicyandiamide; AS+BCH ammonium sulfate associated with root extracts ofBrachiaria humidicola; and AS+SCS ammonium sulfate associated with root extracts of Saccharum spontaneum. Differences were observed in biomass production in plants 45 and 60 days after fertilization (DAF and 15 and 60 days in biomass accumulation of roots. The application of AS associated with DCD synthetic inhibitor kept NO3−-N values low throughout the evaluation period, while in other treatments the concentration increased right up to the second evaluation 15 DAF. Sugarcane plants did not benefit from the increased presence of ammoniacal N promoted by DCD. The use of DCD reduced the average flux of N2O during the evaluation period compared to plants receiving AS treatments only, which was not observed when root extracts of B. humidicola and S. spontaneum were used.

  15. Influence of overstory density on ecophysiology of red oak (Quercus rubra) and sugar maple (Acer saccharum) seedlings in central Ontario shelterwoods

    Science.gov (United States)

    William C. Parker; Daniel C. Dey

    2008-01-01

    A field experiment was established in a secondgrowth hardwood forest dominated by red oak (Quercus rubra L.) to examine the effects of shelterwood overstory density on leaf gas exchange and seedling water status of planted red oak, naturally regenerated red oak and sugar maple (Acer saccharum Marsh.) seedlings during the first...

  16. Estimation of nitrogen fixation in Saccharum spp. by 15N dilution method

    International Nuclear Information System (INIS)

    Singh, Mohan

    1994-01-01

    The amount of nitrogen fixed by bacteria associated with the roots of Saccharum spontaneum, S. sinense, and S. barberi has been estimated by 15 N-isotope dilution method using Sclerotachya fusca as a non-fixing control. S. spontaneum produced highest shoot dry weight among the species tested but maximum nitrogen was accumulated by S. barberi. Highest dilution in the 15 N-enrichment was observed in S. spontaneum followed by S. sinense and S. barberi in comparison to the control plant of Sclerotchya fusca. S. spontaneum derived 60 per cent followed by S. sinense 54 per cent and S. barberi 35 per cent of their total nitrogen requirement through fixation of nitrogen by diazotrophic bacteria associated with their roots. (author). 11 refs., 2 tabs

  17. Length and nucleotide sequence polymorphism at the trnL and trnF non-coding regions of chloroplast genomes among Saccharum and Erianthus species

    Science.gov (United States)

    The aneupolyploidy genome of sugarcane (Saccharum hybrids spp.) and lack of a classical genetic linkage map make genetics research most difficult for sugarcane. Whole genome sequencing and genetic characterization of sugarcane and related taxa are far behind other crops. In this study, universal PCR...

  18. Fiber composition of a diversity panel of the world collection of sugarcane (Saccharum spp. and related grasses

    Directory of Open Access Journals (Sweden)

    James Ryan Todd

    2017-12-01

    Full Text Available ABSTRACT The world collection of sugarcane (Saccharum hybrids. and related grasses (WCSRG is an important source of genes for sugarcane and energycane breeding. The core collection or diversity panel of the WCSRG was created in Canal Point with 300 accession and 10 checks to evaluate its diversity. The fiber components of the species and accessions within the WCSRG are unknown, so a shredded and dried fiber sample was taken from each accession and sent for fiber analysis. The acetyl groups, acid insoluble lignin, acid soluble lignin, arabinan, glucan, holocellulose, total lignin, structural ash, and xylan were quantified on a % fiber basis and nonstructural ash on a % total basis. There were significant, but not large differences between species for holocellulose, lignin, acetyl, acid soluble lignin, nonstructural ash, and glucan. For each trait, Saccharum spontaneum had significantly more holocellulose, glucan, lignin, and nonstructural ash and less acetyl and acid soluble lignin than other species. In all populations, glucan and holocellulose were positively correlated and glucan and lignin were negatively correlated. In hybrids, internode length correlated positively with holocellulose and nonstructural ash and negatively with lignin. Principal component analysis indicated that a large amount of diversity exists within each of the species. The results suggest that strategic use of the WCSRG could provide both positive and negative selection for fiber-related traits could be made within all species of the collection.

  19. Acid-base status of upper rooting zone soil in declining and non-declining sugar maple (Acer saccharum Marsh) stands in Pennsylvania

    Science.gov (United States)

    William E. Sharpe; Troy L. Sunderland

    1995-01-01

    Sugar maple (Acer saccharum Marsh) is an important commercial tree species of the central hardwood region which is valued for its wood and maple sugar products. High elevation sugar maple stands in northcentral Pennsylvania have been in serious decline for about the last 15 years with more than 1,200 hectares of maple forest affected. The decline...

  20. Assessment of Sugarcane Germplasm ( Saccharum spp. complex Against Red Rot Pathogen Colletotrichum Falcatum

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    Atul Singh

    2017-08-01

    Full Text Available ABSTRACT Red rot, caused by Colletotrichum falcatum Went is the most important disease of sugarcane in India inflicting substantial loss to both cane industry and cane growers. To keep in view the importance of red rot disease of sugarcane, 117 accession of sugarcane germplasm including different Saccharum species and Indian and foreign commercial hybrids were tested against red rot with Cf 07, Cf 08 & Cf 09 (national pathotypes by plug method of inoculation. Out of 117, 6 were found resistant and 12 were moderately resistant against red rot and rest were moderately susceptible/susceptible/highly susceptible. Theses resistance and moderately resistant accession can be further utilize to produce resistance varieties against the most devastating pathogen of sugarcane.

  1. Long-term calcium addition increases growth release, wound closure, and health of sugar maple (Acer saccharum) trees at the Hubbard Brook Experimental Forest

    Science.gov (United States)

    Brett A. Huggett; Paul G. Schaberg; Gary J. Hawley; Christopher Eager

    2007-01-01

    We surveyed and wounded forest-grown sugar maple (Acer saccharum Marsh.) trees in a long-term, replicated Ca manipulation study at the Hubbard Brook Experimental Forest in New Hampshire, USA. Plots received applications of Ca (to boost Ca availability above depleted ambient levels) or A1 (to compete with Ca uptake and further reduce Ca availability...

  2. Managing for delicious ecosystem service under climate change: can United States sugar maple (Acer saccharum) syrup production be maintained in a warming climate?

    Science.gov (United States)

    Stephen N. Matthews; Louis R. Iverson

    2017-01-01

    Sugar maple (Acer saccharum) is a highly valued tree in United States (US) and Canada, and its sap when collected from taps and concentrated, makes a delicious syrup. Understanding how this resource may be impacted by climate change and other threats is essential to continue management for maple syrup into the future. Here, we evaluate the current...

  3. Bioconversion potential of Trichoderma viride HN1 cellulase for a lignocellulosic biomass Saccharum spontaneum.

    Science.gov (United States)

    Iqtedar, Mehwish; Nadeem, Mohammad; Naeem, Hira; Abdullah, Roheena; Naz, Shagufta; Qurat ul Ain Syed; Kaleem, Afshan

    2015-01-01

    The industrialisation of lignocellulose conversion is impeded by expensive cellulase enzymes required for saccharification in bioethanol production. Current research undertakes cellulase production from pretreated Saccharum spontaneum through Trichoderma viride HN1 under submerged fermentation conditions. Pretreatment of substrate with 2% NaOH resulted in 88% delignification. Maximum cellulase production (2603 ± 16.39 U/mL/min carboxymethyl cellulase and 1393 ± 25.55 U/mL/min FPase) was achieved at 6% substrate at pH 5.0, with 5% inoculum, incubated at 35°C for 120 h of fermentation period. Addition of surfactant, Tween 80 and metal ion Mn(+2), significantly enhanced cellulase yield. This study accounts proficient cellulase yield through process optimisation by exploiting cheaper substrate to escalate their commercial endeavour.

  4. UV-B-mediated changes on below-ground communities associated with the roots of Acer saccharum

    International Nuclear Information System (INIS)

    Klironomos, J.N.; Allen, M.F.

    1995-01-01

    1. Little is known about how exposure to UV-B radiation affects rhizosphere microbes. Rhizosphere organisms are fed primarily by root-derived substrates and fulfil functions such as mineralization, immobilization, decomposition, pathogeneity and improvement of plant nutrition; they form the base of the below-ground food web. 2. In this study, we exposed Sugar Maple (Acer saccharum) seedlings to UV-B radiation in order to determine if UV-B influences the activities of mycorrhizal and non-mycorrhizal fungi, bacteria and microbe-feeding arthropods in the rhizosphere. 3. Below-ground organisms are greatly affected by UV-B radiation. Overall, carbon-flow in the plant soil system was shifted from a mutualistic-closed, mycorrhizal-dominated system to an opportunist-open, saprobe/pathogen-dominated one. (author)

  5. Soil water effect on crop growth, leaf gas exchange, water and radiation use efficiency of Saccharum spontaneum L. ssp. aegyptiacum (Willd. Hackel in semi-arid Mediterranean environment

    Directory of Open Access Journals (Sweden)

    Danilo Scordia

    2015-12-01

    Full Text Available Great effort has been placed to identify the most suited bioenergy crop under different environments and management practices, however, there is still need to find new genetic resources for constrained areas. For instance, South Mediterranean area is strongly affected by prolonged drought, high vapour pressure deficit (VPD and extremely high temperatures during summertime. In the present work we investigated the soil water effect on crop growth and leaf gas exchange of Saccharum spontaneum L. ssp. aegyptiacum (Willd. Hackel, a perennial, rhizomatous, herbaceous grass. Furthermore, the net increase of biomass production per unit light intercepted [radiation use efficiency (RUE] and per unit water transpired [water use efficiency (WUE] was also studied. To this end a field trial was carried out imposing three levels of soil water availability (I100, I50 and I0, corresponding to 100%, 50% and 0% of ETm restutition under a semi-arid Mediterranean environment. Leaf area index (LAI, stem height, biomass dry matter yield, CO2 assimilation rate, and transpiration rate resulted significantly affected by measurement time and irrigation treatment, with the highest values in I100 and the lowest in I0. RUE was the highest in I100 followed by I50 and I0; on the other hand, WUE was higher in I0 than I50 and I100. At LAI values greater than 2.0, 85% photosynthetically active radiation was intercepted by the Saccharum stand, irrespective of the irrigation treatment. Saccharum spontaneum spp. aegyptiacum is a potential species for biomass production in environment characterized by drought stress, high temperatures and high VPD, as those of Southern Europe and similar semi-arid areas.

  6. The changes in leaf reflectance of sugar maple seedlings (Acer saccharum Marsh) in response to heavy metal stress

    Science.gov (United States)

    Schwaller, M. R.; Schnetzler, C. C.; Marshall, P. E.

    1981-01-01

    The effects of heavy metal stress on leaf reflectance of sugar maple seedlings (Acer saccharum Marsh) are examined. It is found that sugar maple seedlings treated with anomalous amounts of heavy metals in the rooting medium exhibited an increased leaf reflectance over the entire range of investigated wavelengths, from 475 to 1650 nm. These results conform to those of a previous investigation in the wavelengths from 475 to 660nm, but tend to contradict the previous study in the near infrared wavelengths from 1000 to 1650nm. The differences may possible be due to different water regimes in the two investigations.

  7. The changes in leaf reflectance of sugar maple (Acer saccharum Marsh) seedlings in response to heavy metal stress

    Science.gov (United States)

    Schwaller, M. R.; Schnetzler, C. C.; Marshall, P. E.

    1983-01-01

    The effects of heavy metal stress on leaf reflectance of sugar maple seedlings (Acer saccharum Marsh) are examined. It is found that sugar maple seedlings treated with anomalous amounts of heavy metals in the rooting medium exhibited an increased leaf reflectance over the entire range of investigated wavelengths, from 475 to 1650 nm. These results conform to those of a previous investigation in the wavelengths from 475 to 660 nm, but tend to contradict the previous study in the near infrared wavelengths from 1000 to 1650 nm. The differences may possibly be due to different water regimes in the two investigations. Previously announced in STAR as N81-29729

  8. In-vitro regeneration of sugarcane (saccharum officinarum L.)

    International Nuclear Information System (INIS)

    Azu, E.

    2009-01-01

    Sugarcane (Saccharum officinarum L.) cultivars Barbados 46 (B46), Natal Coimbatore 339 (NC0339), Ragna, local sugarcane cultivar (LSC) and Kenana (Kn) series sourced from Kyebi, Akorley, Subriso, Jejeti and the University of Ghana Agriculture Research Station, Kpong, were evaluated for sterilisation, multiplication and rooting. An efficient double sterilisation protocol was achieved by immersing axillary buds pre-treated with Goldazim (active ingredient carbendazim) in 0.2% mercuric chloride for 7 minutes followed by 0.1 % mercuric chloride for 3 minutes. At this optimal sterilisation regime, 70% of the cultured buds were decontaminated. However, post sterilisation survival required the incorporation of 0.002g/L and 0.001g/L of amphotericin Band cefotaxime respectively in the culture medium indicating that the contaminants were endophytic. Evaluation for multiple shoot induction was conducted using Murashige and Skoog (1962) basal salts (MS) medium supplemented with 25g/L sucrose, 2mg/L IAA, 2mg/L GA 3 , 3g/L activated charcoal and varying concentrations (mg/L) of BAP or kinetin. The response of the cultivars to shoot induction varied with Ragna producing the highest number of shoots (2.17) on 5mg/L BAP indicating genotypic differences. Naphthalene acetic acid (NAA) at a concentration of 5mg/L induced the highest frequency (65%) of roots in all the cultivars tested. Post-flask acclimatization and survival of plantlets was high and independent of the age at which regenerated plantlets were transferred to the plant barn for weaning. This procedure could therefore be useful for regenerating sugarcane plantlets as well as provide target tissues for genetic transformation studies (au).

  9. Genetic consequences of selection cutting on sugar maple (Acer saccharum Marshall).

    Science.gov (United States)

    Graignic, Noémie; Tremblay, Francine; Bergeron, Yves

    2016-07-01

    Selection cutting is a treatment that emulates tree-by-tree replacement for forests with uneven-age structures. It creates small openings in large areas and often generates a more homogenous forest structure (fewer large leaving trees and defective trees) that differs from old-growth forest. In this study, we evaluated whether this type of harvesting has an impact on genetic diversity of sugar maple (Acer saccharum Marshall). Genetic diversity among seedlings, saplings, and mature trees was compared between selection cut and old-growth forest stands in Québec, Canada. We found higher observed heterozygosity and a lower inbreeding coefficient in mature trees than in younger regeneration cohorts of both forest types. We detected a recent bottleneck in all stands undergoing selection cutting. Other genetic indices of diversity (allelic richness, observed and expected heterozygosity, and rare alleles) were similar between forest types. We concluded that the effect of selection cutting on the genetic diversity of sugar maple was recent and no evidence of genetic erosion was detectable in Québec stands after one harvest. However, the cumulative effect of recurring applications of selection cutting in bottlenecked stands could lead to fixation of deleterious alleles, and this highlights the need for adopting better forest management practices.

  10. CHEMICAL COMPOSITION AND IN SITU EVALUATION OF FRESH AND ENSILED SUGARCANE (Saccharum officinarum

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    José Andrés Reyes Gutiérrez

    2015-12-01

    Full Text Available This study evaluated chemical composition and in situ degradability of dry matter (DM, organic matter (OM and ruminal pH of fresh (FSC and ensiled (SCS sugarcane (Saccharum officinarum forage diets. In situ digestibility was determined using the nylon bag technique with four cows fitted with a rumen cannula. Cows were fed with fresh or ensiled sugar cane and supplemented with 1 kg of commercial dairy concentrate (18% CP. Ground sample (5g for each sugar cane (FSC, and SCS were incubated in rumen for 0, 8, 12, 24, 36, 48, 72 and 96 h. Treatments were distributed in a completely randomized design with six replicates. ESC showed significant changes (P0.05 was noted. In situ digestibility of organic matter (ISDOM, % was higher (P0.05 at 12 and 76 h of incubation. The ruminal pH showed no differences (P>0.05 between treatments. It is concluded that the silage of sugar cane is an alternative to provide forage in the season of low growth and quality of the grass. Â

  11. Regional growth decline of sugar maple (Acer saccharum) and its potential causes

    Science.gov (United States)

    Bishop, Daniel A.; Beier, Colin M.; Pederson, Neil; Lawrence, Gregory B.; Stella, John C; Sullivan, Timothy J.

    2015-01-01

    Sugar maple (Acer saccharum Marsh) has experienced poor vigor, regeneration failure, and elevated mortality across much of its range, but there has been relatively little attention to its growth rates. Based on a well-replicated dendrochronological network of range-centered populations in the Adirondack Mountains (USA), which encompassed a wide gradient of soil fertility, we observed that the majority of sugar maple trees exhibited negative growth trends in the last several decades, regardless of age, diameter, or soil fertility. Such growth patterns were unexpected, given recent warming and increased moisture availability, as well as reduced acidic deposition, which should have favored growth. Mean basal area increment was greater on base-rich soils, but these stands also experienced sharp reductions in growth. Growth sensitivity of sugar maple to temperature and precipitation was non-stationary during the last century, with overall weaker relationships than expected. Given the favorable competitive status and age structure of the Adirondack sugar maple populations sampled, evidence of widespread growth reductions raises concern over this ecologically and economically important tree. Further study will be needed to establish whether growth declines of sugar maple are occurring more widely across its range.

  12. Eficiencia de las rizobacterias promotoras del crecimiento vegetal (RPCV) en plántulas de caña de azúcar (Saccharum spp.).

    OpenAIRE

    Morgado González, Antonio

    2013-01-01

    Las Rizobacterias Promotoras de Crecimiento Vegetal (RPCV) son una alternativa en la nutrición de plantas cultivadas, ya que estimulan su crecimiento y rendimiento, mejorando la absorción de nutrimentos, el aumento en su movilización, la producción de antibióticos y de reguladores de crecimiento. La caña de azúcar (Saccharum spp.) es un cultivo muy demandante de nutrimentos y de alto rendimiento en México, el uso de las RPCV es fundamental para mejorar su rentabilidad. En esta investigación,...

  13. Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population.

    Science.gov (United States)

    Wai, Ching Man; Zhang, Jisen; Jones, Tyler C; Nagai, Chifumi; Ming, Ray

    2017-10-11

    Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014 worldwide. To improve sugarcane biomass accumulation, we developed an interspecific cross between Saccharum officinarum 'LA Purple' and Saccharum robustum 'MOL5829'. Selected F1 individuals were self-pollinated to generate a transgressive F2 population with a wide range of biomass yield. Leaf and stem internodes of fourteen high biomass and eight low biomass F2 extreme segregants were used for RNA-seq to decipher the molecular mechanism of rapid plant growth and dry weight accumulation. Gene Ontology terms involved in cell wall metabolism and carbohydrate catabolism were enriched among 3274 differentially expressed genes between high and low biomass groups. Up-regulation of cellulose metabolism, pectin degradation and lignin biosynthesis genes were observed in the high biomass group, in conjunction with higher transcript levels of callose metabolic genes and the cell wall loosening enzyme expansin. Furthermore, UDP-glucose biosynthesis and sucrose conversion genes were differentially expressed between the two groups. A positive correlation between stem glucose, but not sucrose, levels and dry weight was detected. We thus postulated that the high biomass sugarcane plants rapidly convert sucrose to UDP-glucose, which is the building block of cell wall polymers and callose, in order to maintain the rapid plant growth. The gene interaction of cell wall metabolism, hexose allocation and cell division contributes to biomass yield.

  14. Involvement of dopaminergic and cholinergic pathways in the induction of yawning and genital grooming by the aqueous extract of Saccharum officinarum L. (sugarcane) in rats.

    Science.gov (United States)

    Gamberini, Maria T; Gamberini, Maria C; Nasello, Antonia G

    2015-01-01

    Yawning, associated with genital grooming, is a physiological response that may be used for elucidating the mechanism of action of drugs. Preliminary analysis showed that aqueous extract (AE) of Saccharum induced yawns in rats. So, we aimed to quantify these behavioral responses and investigate the pharmacological mechanisms involved in these actions. During 120 min, after AE administration, the yawns and the genital grooming were quantified at 10 min intervals. Since dopaminergic and cholinergic pathways are implied in these responses, AE were evaluated in the presence of haloperidol 0.5 mg/kg and atropine 2 mg/kg. AE 0.5 g/kg increased the yawns, effect that was blocked both by haloperidol and atropine. Genital grooming could only be stimulated by AE 0.5 g/kg when dopaminergic receptors were blocked by haloperidol. However, it was inhibited when atropine was previously administered. So, we demonstrated a central action of Saccharum and it was postulated that neural circuits with the participation of dopaminergic and cholinergic pathways are involved. The fact that AE is comprised of innumerous compounds could justify the extract's distinct responses. Also, we cannot disregard the presence of different neural circuits that count on the participation of dopaminergic and cholinergic pathways and could be activated by the same induction agent. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  15. Characterization of indigoidine biosynthetic genes in Erwinia chrysanthemi and role of this blue pigment in pathogenicity.

    Science.gov (United States)

    Reverchon, Sylvie; Rouanet, Carine; Expert, Dominique; Nasser, William

    2002-02-01

    In the plant-pathogenic bacterium Erwinia chrysanthemi production of pectate lyases, the main virulence determinant, is modulated by a complex network involving several regulatory proteins. One of these regulators, PecS, also controls the synthesis of a blue pigment identified as indigoidine. Since production of this pigment is cryptic in the wild-type strain, E. chrysanthemi ind mutants deficient in indigoidine synthesis were isolated by screening a library of Tn5-B21 insertions in a pecS mutant. These ind mutations were localized close to the regulatory pecS-pecM locus, immediately downstream of pecM. Sequence analysis of this DNA region revealed three open reading frames, indA, indB, and indC, involved in indigoidine biosynthesis. No specific function could be assigned to IndA. In contrast, IndB displays similarity to various phosphatases involved in antibiotic synthesis and IndC reveals significant homology with many nonribosomal peptide synthetases (NRPS). The IndC product contains an adenylation domain showing the signature sequence DAWCFGLI for glutamine recognition and an oxidation domain similar to that found in various thiazole-forming NRPS. These data suggest that glutamine is the precursor of indigoidine. We assume that indigoidine results from the condensation of two glutamine molecules that have been previously cyclized by intramolecular amide bond formation and then dehydrogenated. Expression of ind genes is strongly derepressed in the pecS background, indicating that PecS is the main regulator of this secondary metabolite synthesis. DNA band shift assays support a model whereby the PecS protein represses indA and indC expression by binding to indA and indC promoter regions. The regulatory link, via pecS, between indigoidine and virulence factor production led us to explore a potential role of indigoidine in E. chrysanthemi pathogenicity. Mutants impaired in indigoidine production were unable to cause systemic invasion of potted Saintpaulia ionantha

  16. Caracterização bioquímica de cultivares de cana-de-açúcar (Saccharum spp.: isoenzimas, proteína solúvel e valor brix Biochemical characterization of sugarcane (Saccharum spp. cultivars: isoenzymes, solubre protein and brix value

    Directory of Open Access Journals (Sweden)

    M. de Almeida

    1994-12-01

    Full Text Available No presente trabalho foram determinados o perfil isoenzimático diferencial de esterase e peroxidase, a proteína total solúvel e os sólidos solúveis (sacarose em graus brix, de 10 cultivares de cana-de-açúcar (Saccharum spp. atualmente cultivados no Brasil. Os cultivares estudados foram: NA 56-79, IAC 52-150, IAC 64-257, SP 70-1143, SP 71-3146, SP 71-3149, SP 71-1406, SP 71-6163, SP 71-61-68 e SP 71-799. Com os dados obtidos foi possível comprovar o valor taxonômico das características bioquímicas que representam uma inovação em taxonomia de cana-de-açúcar no Brasil. As isoenzimas de esterase apresentaram um padrão eletroforético específico para cada cultivar estudado, enquanto que as isoenzimas de peroxidase só permitiram agrupar os cultivares por apresentarem o mesmo padrão eletroforético para cada grupo formado. Tanto as isoenzimas de esterase como peroxidase apresentaram-se constantes em um mesmo cultivar. Os sólidos solúveis (sacarose em Graus Brix e a determinação da proteína solúvel, mesmo sendo pouco variáveis, apresentaram-se úteis para a caracterização dos cultivares estudados.In the present work the isoenzyme profiles of the enzymes esterase and peroxidase, the level of total soluble protein and the soluble solids (sucrose were determined of the following sugarcane (Saccharum spp. cultivare: NA 56-79; IAC 52-150; IAC 64-257; SP 70-1143; SP 71-3146; SP 71-3149; SP 71-1406; SP 71-6163; SP 71-61-68 and SP 71-799. Esterase isoenzymes showed a specific electrophoretic pattern for each one of the cultivare, while the peroxidase allowed to arrange the cultivare in groups, each one with a specific electrophoretic pattern. The isoenzymes of both esterase and peroxidase were constant in a given cultivar. Total soluble protein levels and soluble solids (sucrose, Brix value varied among the cultivare. Statistical analysis showed that these biochemical parameters are useful for the characterization of the cultivare

  17. Growth and metal uptake of energy sugarcane (Saccharum spp.) in different metal mine tailings with soil amendments.

    Science.gov (United States)

    Zhang, Xin; Zhu, Yongguan; Zhang, Yuebin; Liu, Yunxia; Liu, Shaochun; Guo, Jiawen; Li, Rudan; Wu, Songlin; Chen, Baodong

    2014-05-01

    A pot experiment was conducted to investigate the feasibility of growing energy sugarcane (Saccharum spp.) in three different metal mine tailings (Cu, Sn and Pb/Zn tailings) amended with uncontaminated soil at different mixing ratios. The results indicated that sugarcane was highly tolerant to tailing environments. Amendments of 20% soil to Sn tailings and 30% soil to Cu tailings could increase the biomass of cane-stem for use as the raw material for bioethanol production. Heavy metals were mostly retained in roots, which indicated that sugarcane was useful for the stabilization of the tailings. Bagasse and juice, as the most valuable parts to produce bioethanol, only accounted for 0.6%-3% and 0.6%-7% of the total metal content. Our study supported the potential use of sugarcane for tailing phytostabilization and bioenergy production. Copyright © 2014 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

  18. Physiological and proteomic analyses of Saccharum spp. grown under salt stress.

    Directory of Open Access Journals (Sweden)

    Aline Melro Murad

    Full Text Available Sugarcane (Saccharum spp. is the world most productive sugar producing crop, making an understanding of its stress physiology key to increasing both sugar and ethanol production. To understand the behavior and salt tolerance mechanisms of sugarcane, two cultivars commonly used in Brazilian agriculture, RB867515 and RB855536, were submitted to salt stress for 48 days. Physiological parameters including net photosynthesis, water potential, dry root and shoot mass and malondialdehyde (MDA content of leaves were determined. Control plants of the two cultivars showed similar values for most traits apart from higher root dry mass in RB867515. Both cultivars behaved similarly during salt stress, except for MDA levels for which there was a delay in the response for cultivar RB867515. Analysis of leaf macro- and micronutrients concentrations was performed and the concentration of Mn(2+ increased on day 48 for both cultivars. In parallel, to observe the effects of salt stress on protein levels in leaves of the RB867515 cultivar, two-dimensional gel electrophoresis followed by MS analysis was performed. Four proteins were differentially expressed between control and salt-treated plants. Fructose 1,6-bisphosphate aldolase was down-regulated, a germin-like protein and glyceraldehyde 3-phosphate dehydrogenase showed increased expression levels under salt stress, and heat-shock protein 70 was expressed only in salt-treated plants. These proteins are involved in energy metabolism and defense-related responses and we suggest that they may be involved in protection mechanisms against salt stress in sugarcane.

  19. Desarrollo de sistema de información geográfica para gestión fitosanitaria en unidades productivas de caña de azúcar (Saccharum spp.)

    OpenAIRE

    Balmaseda, C; Romero, Madelin

    2012-01-01

    La fitoprotección del cultivo de la caña de azúcar (Saccharum spp.) requiere de la aplicación de modernas herramientas y procedimientos tecnológicos con la finalidad de mantener la vigilancia y facilitar el proceso de toma de decisiones en el control fitosanitario. Para ello se desarrolló un sistema de información geográfica (SIG) eligiendo como base cartográfica el catastro especializado de una empresa. El modelo conceptual desarrollado muestra las relaciones que existen entre variables cons...

  20. Avaliação da divergência nutricional de variedades de cana-de-açúcar (Saccharum spp. Evaluation of the nutritional divergence of the sugarcane (Saccharum spp. varieties

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    José Augusto Gomes Azevêdo

    2003-12-01

    Full Text Available O objetivo do trabalho foi avaliar a divergência nutricional de variedades de cana-de-açúcar, utilizando a análise de componentes principais, visando identificar três variedades representativas dessa divergência. As variedades de cana-de-açúcar (Saccharum spp. avaliadas neste estudo foram: RB855113, RB765418, RB855536, SP79-2233, RB845257, SP80-180, RB855453, RB855336, SP80-1842, SP81-1763, SP80-4445, SP79-1011, RB739359, RB867515 e SP80-3280, colhidas aos 426, 487 e 549 dias após o plantio. As variáveis discriminatórias utilizadas foram fibra em detergente neutro (FDN, hemicelulose, lignina, fração indegradável da FDN e taxa de degradação da fração potencialmente degradável da FDN. Houve diferenças para todas as variáveis estudadas, exceto para a fração indegradável da FDN. A avaliação da divergência nutricional das variedades de cana-de-açúcar baseou-se nos três primeiros componentes principais, explicando 87,8% da variação total. A FDN e a fração indegradável da FDN foram as variáveis de menor importância para explicar a variabilidade nutricional das variedades. A variedade SP80-1842, colhida aos 426 dias após o plantio, e a variedade SP79-1011, colhida aos 549 dias, foram as que apresentaram maior dispersão dos escores nos três primeiros componentes principais, sendo consideradas as mais dissimilares, enquanto a variedade RB845257, colhida aos 487 dias após o plantio, localizou-se em posição intermediária entre ambas. A análise de componentes principais foi também eficiente em selecionar as variedades com diferentes épocas de colheita.The objective of this work was to evaluate the nutritional divergence of the sugarcane varieties, using the principal components analysis, to select three representative varieties of that divergence. The sugarcane varieties (Saccharum spp. evaluated in this study were: RB855113, RB765418, RB855536, SP79-2233, RB845257, SP80-180, RB855453, RB855336, SP80-1842, SP81

  1. Alleviation of Salinity Effects by Exogenous Applications of Salicylic Acid in Sugarcane (Saccharum officinarum L. Seedlings

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    F Chaharlang Badil

    2016-12-01

    Full Text Available Introduction Abiotic stresses cause 71% reduction in crop yield around the world, from which 20% is related to salinity stress. The importance of sugarcane increases every day due to greater demand for sugar. Since sugarcane has mainly grown in arid and semi-arid regions, salinity is one of the main problems for this crop due to higher evaporation in these areas. Salicylic acid (SA is classified as a phyto – hormone and belongs to a group of phenol compounds. Salicylic acid can improve plant tolerance to abiotic stresses. This research aimed at studying the effect of SA on the alleviating of salinity stress in sugarcane. Materials and Methods The effects of salicylic acid on the growth and some physiological responses of sugarcane (Saccharum officinarum L. cv. CP69-1062 were studied under salt stress. The experiment design was a factorial of two factors, based on a randomized completely design with three replications. The experiment was conducted in a greenhouse at the Sugarcane Research and Training Institute of Khuzestan, Iran in 2012. Treatments evaluated in this study were three levels of salt stress, including (ECW

  2. Contributions of leaf photosynthetic capacity, leaf angle and self-shading to the maximization of net photosynthesis in Acer saccharum: a modelling assessment.

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    Posada, Juan M; Sievänen, Risto; Messier, Christian; Perttunen, Jari; Nikinmaa, Eero; Lechowicz, Martin J

    2012-08-01

    Plants are expected to maximize their net photosynthetic gains and efficiently use available resources, but the fundamental principles governing trade-offs in suites of traits related to resource-use optimization remain uncertain. This study investigated whether Acer saccharum (sugar maple) saplings could maximize their net photosynthetic gains through a combination of crown structure and foliar characteristics that let all leaves maximize their photosynthetic light-use efficiency (ε). A functional-structural model, LIGNUM, was used to simulate individuals of different leaf area index (LAI(ind)) together with a genetic algorithm to find distributions of leaf angle (L(A)) and leaf photosynthetic capacity (A(max)) that maximized net carbon gain at the whole-plant level. Saplings grown in either the open or in a forest gap were simulated with A(max) either unconstrained or constrained to an upper value consistent with reported values for A(max) in A. saccharum. It was found that total net photosynthetic gain was highest when whole-plant PPFD absorption and leaf ε were simultaneously maximized. Maximization of ε required simultaneous adjustments in L(A) and A(max) along gradients of PPFD in the plants. When A(max) was constrained to a maximum, plants growing in the open maximized their PPFD absorption but not ε because PPFD incident on leaves was higher than the PPFD at which ε(max) was attainable. Average leaf ε in constrained plants nonetheless improved with increasing LAI(ind) because of an increase in self-shading. It is concluded that there are selective pressures for plants to simultaneously maximize both PPFD absorption at the scale of the whole individual and ε at the scale of leaves, which requires a highly integrated response between L(A), A(max) and LAI(ind). The results also suggest that to maximize ε plants have evolved mechanisms that co-ordinate the L(A) and A(max) of individual leaves with PPFD availability.

  3. Development of novel genic microsatellite markers from transcriptome sequencing in sugar maple (Acer saccharum Marsh.).

    Science.gov (United States)

    Harmon, Monica; Lane, Thomas; Staton, Margaret; Coggeshall, Mark V; Best, Teodora; Chen, Chien-Chih; Liang, Haiying; Zembower, Nicole; Drautz-Moses, Daniela I; Hwee, Yap Zhei; Schuster, Stephan C; Schlarbaum, Scott E; Carlson, John E; Gailing, Oliver

    2017-08-08

    Sugar maple (Acer saccharum Marsh.) is a hardwood tree species native to northeastern North America and economically valued for its wood and sap. Yet, few molecular genetic resources have been developed for this species to date. Microsatellite markers have been a useful tool in population genetics, e.g., to monitor genetic variation and to analyze gene flow patterns. The objective of this study is to develop a reference transcriptome and microsatellite markers in sugar maple. A set of 117,861 putative unique transcripts were assembled using 29.2 Gb of RNA sequencing data derived from different tissues and stress treatments. From this set of sequences a total of 1068 microsatellite motifs were identified. Out of 58 genic microsatellite markers tested on a population of 47 sugar maple trees in upper Michigan, 22 amplified well, of which 16 were polymorphic and 6 were monomorphic. Values for expected heterozygosity varied from 0.224 to 0.726 for individual loci. Of the 16 polymorphic markers, 15 exhibited transferability to other Acer L. species. Genic microsatellite markers can be applied to analyze genetic variation in potentially adaptive genes relative to genomic reference markers as a basis for the management of sugar maple genetic resources in the face of climate change.

  4. Winter climate change and fine root biogenic silica in sugar maple trees (Acer saccharum): Implications for silica in the Anthropocene

    Science.gov (United States)

    Maguire, Timothy J.; Templer, Pamela H.; Battles, John J.; Fulweiler, Robinson W.

    2017-03-01

    Winter temperatures are projected to increase over the next century, leading to reductions in winter snowpack and increased frequency of soil freezing in many northern forest ecosystems. Here we examine biogenic silica (BSi) concentrations in sugar maple (Acer saccharum) fine roots collected from a snow manipulation experiment at Hubbard Brook Experimental Forest (New Hampshire, USA). Increased soil freezing significantly lowered the BSi content of sugar maple fine roots potentially decreasing their capacity to take up water and dissolved nutrients. The reduced silica uptake (8 ± 1 kmol silica km-2) by sugar maple fine roots is comparable to silica export from temperate forest watersheds. We estimate that fine roots account for 29% of sugar maple BSi, despite accounting for only 4% of their biomass. These results suggest that increased frequency of soil freezing will reduce silica uptake by temperate tree roots, thereby changing silica availability in downstream receiving waters.

  5. Effects of ion beam irradiation on adventitious shoot regeneration from in vitro leaf explants of Septennial ionahta

    International Nuclear Information System (INIS)

    Zhou, L.B.; Li, W.J.; Ma, S.; Dong, X.C.; Yu, L.X.; Li, Q.; Zhou, G.M.; Gao, Q.X.

    2006-01-01

    The effects of 960 MeV carbon ion beam and 8 MeV X-ray irradiation on adventitious shoots from in vitro leaf explants of two different Saintpaulia ionahta (Mauve and Indikon) cultivars were studied with regard to tissue increase, shoots differentiation and morphology changes in the shoots. The experimental results showed that the survival fraction of shoot formation for the Mauve and Indikon irradiated with the carbon ion beam at 20 Gy were 0.715 and 0.600, respectively, while those for both the cultivars exposed to the X-ray irradiation at the same dose were 1.000. Relative biological effectiveness (RBE) of Mauve with respect to X-ray was about two. Secondly, the percentage of regenerating explants with malformed shoots in all Mauve regenerating explants irradiated with carbon ion beam at 20 Gy accounted for 49.6%, while that irradiated with the same dose of X-ray irradiation was only 4.7%; as for Saintpaulia ionahta Indikon irradiated with 20 Gy carbon ion beam, the percentage was 43.3%, which was higher than that of X-ray irradiation. Last, many chlorophyll deficient and other varieties of mutants were obtained in this study. Based on the results above, it can be concluded that the effect of mutation induction by carbon ion beam irradiation on the leaf explants of Saintpaulia ionahta is better than that by X-ray irradiation; and the optimal mutagenic dose varies from 20 Gy to 25 Gy for carbon ion beam irradiation

  6. Soft rot erwiniae: from genes to genomes.

    Science.gov (United States)

    Toth, Ian K; Bell, Kenneth S; Holeva, Maria C; Birch, Paul R J

    2003-01-01

    SUMMARY The soft rot erwiniae, Erwinia carotovora ssp. atroseptica (Eca), E. carotovora ssp. carotovora (Ecc) and E. chrysanthemi (Ech) are major bacterial pathogens of potato and other crops world-wide. We currently understand much about how these bacteria attack plants and protect themselves against plant defences. However, the processes underlying the establishment of infection, differences in host range and their ability to survive when not causing disease, largely remain a mystery. This review will focus on our current knowledge of pathogenesis in these organisms and discuss how modern genomic approaches, including complete genome sequencing of Eca and Ech, may open the door to a new understanding of the potential subtlety and complexity of soft rot erwiniae and their interactions with plants. The soft rot erwiniae are members of the Enterobacteriaceae, along with other plant pathogens such as Erwinia amylovora and human pathogens such as Escherichia coli, Salmonella spp. and Yersinia spp. Although the genus name Erwinia is most often used to describe the group, an alternative genus name Pectobacterium was recently proposed for the soft rot species. Ech mainly affects crops and other plants in tropical and subtropical regions and has a wide host range that includes potato and the important model host African violet (Saintpaulia ionantha). Ecc affects crops and other plants in subtropical and temperate regions and has probably the widest host range, which also includes potato. Eca, on the other hand, has a host range limited almost exclusively to potato in temperate regions only. Disease symptoms: Soft rot erwiniae cause general tissue maceration, termed soft rot disease, through the production of plant cell wall degrading enzymes. Environmental factors such as temperature, low oxygen concentration and free water play an essential role in disease development. On potato, and possibly other plants, disease symptoms may differ, e.g. blackleg disease is associated

  7. Evolution of cytotoxicity, antioxidant, and antimicrobial studies of sugarcane (saccharum officinarum) roots extracts

    International Nuclear Information System (INIS)

    Khaliq, M.; Riaz, M.; Kanwal, N.; Mahmood, I.; Khan, A.

    2017-01-01

    For health care over three-quarters of the world population mainly relies on plants and plant extracts. In this research work percentage yield, qualitative and quantitative analysis of phytochemicals, antioxidant ability, antimicrobial activity and cytotoxic effects of sugarcane (Saccharum officinarum) root extracts were analyzed. Roots of selected plant were extracted by socking in polarity base solvents i.e. n-hexane, chloroform, ethyl acetate, n-butanol and methanol. As a result of the qualitative determination of phytochemicals flavonoids, saponin, tannins and phlobatannins found to be present, while alkaloids, steroids, cardiac glycosides and terpenoids found to be absent. Spectroscopic methods were used to determine total phenolic contents 7.58-126.04 Gallic acid equivalent mg/100g of dry extracts and flavonoid contents 1.71-25.4 Catechin equivalent mg/100g of dry extracts. The antioxidant capacity of plant extracts of free radical scavenging by DPPH, percentage inhibition of linoleic acid oxidation and reducing power assays were carried out. BHT was used as a positive control in the percentage scavenging assay. The percentage free radical scavenging by DPPH (having concentration 0.025 g/L) was found in the range of 80.13-86.74 %. The percentage inhibition of linoleic acid oxidation was also analysed. Antimicrobial activity was studied in all extracts against selected bacteria (Escherichia coli and Bacillus subtilis) and fungal strains (Aspergillus niger and Rhizopus solani). Hemolytic activity was also determined in plant extracts and maximum percentage of lysis was found as 1.582 % by methanol extract, while the minimum percentage of lysis was 0.010 % for n-hexane extract. (author)

  8. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum

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    JUAN P.P. LLERENA

    Full Text Available ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great difficulty in RT-PCR reactions. Thus, we evaluated the effectiveness of different additives in the amplification of these genes. While COMT and CCoAOMT genes did not need any additives for other genes there was no amplification (HCT, F5H, 4CL and CCR or the yield was very low (CAD and C4H. The application of supplementary cDNA was enough to overcome the non-specificity and low yield for C4H and C3H, while the addition of 0.04% BSA + 2% formamide was effective to amplify 4CL, CCR, F5H and CCR. HCT was amplified only by addition of 0.04% BSA + 2% formamide + 0.1 M trehalose and amplification of PAL was possible with addition of 2% of DMSO. Besides optimization of expression assays, the results show that additives can act independently or synergistically.

  9. Arbuscular mycorrhizal association enhances drought tolerance potential of promising bioenergy grass (Saccharum arundinaceum retz.).

    Science.gov (United States)

    Mirshad, P P; Puthur, Jos T

    2016-07-01

    The influence of arbuscular mycorrhizal fungi (AMF) (Glomus spp.) on some physiological and biochemical characteristics of bioenergy grass Saccharum arundinaceum subjected to drought stress was studied. The symbiotic association of Glomus spp. was established with S. arundinaceum, a potential bioenergy grass as evident from the increase in percentage of root infection and distribution frequency of vesicles when compared with non-arbuscular mycorrhizal plants. AMF-treated plants exhibited an enhanced accumulation of osmolytes such as sugars and proline and also increased protein content under drought. AMF association significantly increased the accumulation of non-enzymatic antioxidants like phenols, ascorbate and glutathione as well as enhanced the activities of antioxidant enzymes such as SOD (superoxide dismutase), APX (ascorbate peroxidase) and GPX (guaiacol peroxidase) resulting in reduced lipid peroxidation in S. arundinaceum. AMF symbiosis also ameliorated the drought-induced reduction of total chlorophyll content and activities of photosystem I and II. The maximum quantum efficiency of PS II (F v/F m) and potential photochemical efficiency (F v/F o) were higher in AMF plants as compared to non-AMF plants under drought stress. These results indicate that AMF association alleviate drought stress in S. arundinaceum by the accumulation of osmolytes and non-enzymatic antioxidants and enhanced activities of antioxidant enzymes, and hence, the photosynthetic efficiency is improved resulting in increased biomass production. AMF association with energy grasses also improves the acclimatization of S. arundinaceum for growing in marginal lands of drought-affected soils.

  10. Evaluation of Sugarcane (Saccharum officinarum L. Somaclonals Tolerance to Salinity Via In Vitro and In Vivo

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    HAMID RAJABI MEMARI

    2011-06-01

    Full Text Available Tissue culture technique was used to obtain salt tolerant variants from embryogenic calluses of sugarcane (Saccharum sp. var. CP48-103 that cultured on a selective medium containing different levels of NaCl (0, 0.2, 0.4, 0.6, and 0.8% NaCl. A total of four plants regenerated from the tolerant calluses were selected but the best of them in vigor grown in in vitro and hydroponic systems under salinity stress to comparison with source variety. With increasing supply of NaCl in both systems, root growth was more adversely affected than was shoot growth. Chlorophyll contents showed a decreasing trend and dry matter yield of plants reduced but in a slow rate in tolerant somaclonal than source variety. The biochemical analysis showed that at high salt concentration, Cl- and Na+ content in shoot and root increased. With rising salt concentration from 0 to 0.8%, content of Cl- in shoot and root of tolerant variant changed lower than parent showed that this variant had genetic lowest ratio of shoot/root chloride and had minimum transport of Cl- to shoots. Also this variant had high content of Ca2+ in shoot and high K+/Na+ ratio at all salinity levels. Thus it probably has genetic potential to avoid harmful ions uptake.

  11. microRNAs Associated with Drought Response in the Bioenergy Crop Sugarcane (Saccharum spp.)

    Science.gov (United States)

    Vilela, Romel Duarte; Costa, Gustavo Gilson Lacerda; Dias, Lara Isys; Endres, Laurício; Menossi, Marcelo

    2012-01-01

    Sugarcane (Saccharum spp.) is one of the most important crops in the world. Drought stress is a major abiotic stress factor that significantly reduces sugarcane yields. However the gene network that mediates plant responses to water stress remains largely unknown in several crop species. Although several microRNAs that mediate post-transcriptional regulation during water stress have been described in other species, the role of the sugarcane microRNAs during drought stress has not been studied. The objective of this work was to identify sugarcane miRNAs that are differentially expressed under drought stress and to correlate this expression with the behavior of two sugarcane cultivars with different drought tolerances. The sugarcane cultivars RB867515 (higher drought tolerance) and RB855536 (lower drought tolerance) were cultivated in a greenhouse for three months and then subjected to drought for 2, 4, 6 or 8 days. By deep sequencing of small RNAs, we were able to identify 18 miRNA families. Among all of the miRNAs thus identified, seven were differentially expressed during drought. Six of these miRNAs were differentially expressed at two days of stress, and five miRNAs were differentially expressed at four days. The expression levels of five miRNAs (ssp-miR164, ssp-miR394, ssp-miR397, ssp-miR399-seq 1 and miR528) were validated by RT-qPCR (quantitative reverse transcriptase PCR). Six precursors and the targets of the differentially expressed miRNA were predicted using an in silico approach and validated by RT-qPCR; many of these targets may play important roles in drought tolerance. These findings constitute a significant increase in the number of identified miRNAs in sugarcane and contribute to the elucidation of the complex regulatory network that is activated by drought stress. PMID:23071617

  12. Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.

    Directory of Open Access Journals (Sweden)

    Ashok A. Nikam

    2015-02-01

    Full Text Available Gamma ray-induced in vitro mutagenesis and selection for salt (NaCl tolerance were investigated in sugarcane (Saccharum officinarum L.. Embryogenic callus cultures were irradiated (10 to 80 Gy and subjected to in vitro selection by exposure of irradiated callus to NaCl (0, 50, 100, 150, 200, and 250 mmol L− 1. Increasing NaCl concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+ and K+ concentration. Higher accumulation of proline and glycine betaine was observed in NaCl stressed callus irradiated at 20 Gy. Na+ concentration increased and K+ concentration decreased with increasing salt level. Irradiated callus showed 50–60% regeneration under NaCl stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%, number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane.

  13. Composition, Taste, Aroma, and Antioxidant Activity of Solidified Noncentrifugal Brown Sugars Prepared from Whole Stalk and Separated Pith of Sugarcane (Saccharum officinarum L.).

    Science.gov (United States)

    Takahashi, Makoto; Ishmael, Mutanda; Asikin, Yonathan; Hirose, Naoto; Mizu, Masami; Shikanai, Takesi; Tamaki, Hajime; Wada, Koji

    2016-10-25

    In this study, 2 types of solidified noncentrifugal brown sugars (W-NCS and P-NCS) were prepared from the whole stalk and separated pith, respectively, of raw sugarcane (Saccharum officinarum L.). These products were discriminated in terms of their quality attributes, including color, sugars and minerals composition, taste, aroma, and antioxidant activity. The brown color of P-NCS was clearly different compared with that of W-NCS with a color difference value (ΔE * ) of 9.36. There was no difference in the sugars and minerals composition between the 2 types of sugar, which led to very similar taste profiles. However, P-NCS had a weaker aroma intensity than W-NCS did. Moreover, P-NCS retained more than 60% of the antioxidant activity of W-NCS. The information gleaned from this study might be used to select appropriate end-uses for these 2 types of sugars. © 2016 Institute of Food Technologists®.

  14. Development of corn starch based green composites reinforced with Saccharum spontaneum L fiber and graft copolymers--evaluation of thermal, physico-chemical and mechanical properties.

    Science.gov (United States)

    Kaith, B S; Jindal, R; Jana, A K; Maiti, M

    2010-09-01

    In this paper, corn starch based green composites reinforced with graft copolymers of Saccharum spontaneum L. (Ss) fiber and methyl methacrylates (MMA) and its mixture with acrylamide (AAm), acrylonitrile (AN), acrylic acid (AA) were prepared. Resorcinol-formaldehyde (Rf) was used as the cross-linking agent in corn starch matrix and different physico-chemical, thermal and mechanical properties were evaluated. The matrix and composites were found to be thermally more stable than the natural corn starch backbone. Further the matrix and composites were subjected for biodegradation studies through soil composting method. Different stages of biodegradation were evaluated through FT-IR and scanning electron microscopic (SEM) techniques. S. spontaneum L fiber-reinforced composites were found to exhibit better tensile strength. On the other hand Ss-g-poly (MMA) reinforced composites showed maximum compressive strength and wear resistance than other graft copolymers reinforced composite and the basic matrix. (c) 2010 Elsevier Ltd. All rights reserved.

  15. One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells

    Energy Technology Data Exchange (ETDEWEB)

    Mehta, Vaibhavkumar N. [Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007 (India); Jha, Sanjay [Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007 (India); Kailasa, Suresh Kumar, E-mail: sureshkumarchem@gmail.com [Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007 (India)

    2014-05-01

    We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ∼ 3 nm and showed bright blue fluorescence under UV-light (λ{sub ex} = 365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae). - Highlights: • One-pot green synthesis was used for fluorescent CDs. • FT-IR, DLS, and TEM were used for the characterization of CDs. • The CDs are well dispersed in water with an average size of ∼ 3 nm. • The CDs acted as fluorescent probes for imaging of bacteria and yeast cells.

  16. Light drives vertical gradients of leaf morphology in a sugar maple (Acer saccharum) forest.

    Science.gov (United States)

    Coble, Adam P; Cavaleri, Molly A

    2014-02-01

    Leaf mass per area (LMA, g m(-2)) is an essential trait for modeling canopy function due to its strong association with photosynthesis, respiration and leaf nitrogen. Leaf mass per area, which is influenced by both leaf thickness and density (LMA = thickness × density), generally increases from the bottom to the top of tree canopies, yet the mechanisms behind this universal pattern are not yet resolved. For decades, the light environment was assumed to be the most influential driver of within-canopy variation in LMA, yet recent evidence has shown hydrostatic gradients to be more important in upper canopy positions, especially in tall evergreen trees in temperate and tropical forests. The aim of this study was to disentangle the importance of various environmental drivers on vertical LMA gradients in a mature sugar maple (Acer saccharum Marshall) forest. We compared LMA, leaf density and leaf thickness relationships with height, light and predawn leaf water potential (ΨPre) within a closed and an exposed canopy to assess leaf morphological traits at similar heights but different light conditions. Contrary to our expectations and recent findings in the literature, we found strong evidence that light was the primary driver of vertical gradients in leaf morphology. At similar heights (13-23 m), LMA was greater within the exposed canopy than the closed canopy, and light had a stronger influence over LMA compared with ΨPre. Light also had a stronger influence over both leaf thickness and density compared with ΨPre; however, the increase in LMA within both canopy types was primarily due to increasing leaf thickness with increasing light availability. This study provides strong evidence that canopy structure and crown exposure, in addition to height, should be considered as a parameter for determining vertical patterns in LMA and modeling canopy function.

  17. Competição entre quatro herbicidas indicados para o controle de plantas daninhas em cana-de-açucar (Saccharum sp Competition on four herbicides for controling weeds in sugarcane (Saccharum sp

    Directory of Open Access Journals (Sweden)

    L.S.P. Cruz

    1978-09-01

    Full Text Available Com a finalidade de estudar a ação dos herbicidas oxadiazon, napropamide, methazole e A-3587 na cultura de cana -de-açúcar (Saccharum sp, foram conduzidos em 1971/72 dois ensaios de campo nos municípios paulistas de Capivari e Leme. Os herbicidas acima citados foram comparados, em trés doses, com 2,4-D anina e atrazine, aplicados em pré -emergência. Oxadiazon a 2,0 kg/ha e A-3587 a 3,0 kg e 4,0 kg/ha foram eficientes no controle das principais plantas daninhas que apareceram nos experimentos: capim-marmelada, Brachiaria plantaginea (Link Hitch; capim-de-colchão, Digitaria sanguinalis (L. Scop; capim-pé-degalinha, Eleusine indica (L. Gaertn.; beldroega, Portulacca oleracea L e guanxumas, Sida spp. Napropamide a 3,0 kg/ha e methazole a 3,5 kg e 4,0 kg/ha só não con trolaram as guanxumas. Nas doses empregadas, nenhum dos herbicidas mostrou-se prejudicial às plantas e à produção de cana-de-açúcar.This paper reports on two field trials carried out during 1971/72, in Capivari and Leme, with the herbicides oxadiazon, napropamide, methazole and A-3587, in sugar-cane crops. In these experiments the four herbicides, in three doses, were compared, in preemergence, with 2,4-D amine and atrazine. Oxadiazon at 2.0 kg/ha and A-3587 at 3.0 kg and 4,0 kg/ha gave good control against Brachiaria plantaginea, Digitaria sanguinalis, Eleusine indica, Portulacca oleracea and Sida spp, wich were found in the trials. Napropamide at 3.0 kg/ha and methazole at 3.5 kg and 4.0 kg/ha gave good control against those weeds but except Sida spp. None of the herbicides caused injuries to the crop or reduced yield.

  18. Analyses of Indole Compounds in Sugar Cane (Saccharum officinarum L. Juice by High Performance Liquid Chromatography and Liquid Chromatography-Mass Spectrometry after Solid-Phase Extraction

    Directory of Open Access Journals (Sweden)

    Jean Wan Hong Yong

    2017-03-01

    Full Text Available Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins and some of its metabolites, by high performance liquid chromatography (HPLC and liquid chromatography-mass spectrometry (LC-MS after solid-phase extraction (SPE was reported for the first time. The analysis was carried out using a reverse phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid modified by methanol. Furthermore, a novel SPE procedure was developed for the pre-concentration and purification of indole compounds using C18 SPE cartridges. The combination of SPE, HPLC, and LC-MS was applied to screen for the indole compounds present in sugar cane (Saccharum officinarum L. juice, a refreshing beverage with various health benefits. Finally, four indole compounds were successfully detected and quantified in sugar cane juice by HPLC, which were further unequivocally confirmed by LC-MS/MS experiments operating in the multiple reaction monitoring (MRM mode.

  19. Pyrolysis and kinetic analyses of a perennial grass (Saccharum ravannae L.) from north-east India: Optimization through response surface methodology and product characterization.

    Science.gov (United States)

    Saikia, Ruprekha; Baruah, Bhargav; Kalita, Dipankar; Pant, Kamal K; Gogoi, Nirmali; Kataki, Rupam

    2018-04-01

    The objective of the present investigation was to optimize the pyrolysis condition of an abundantly available and low cost perennial grass of north-east India Saccharum ravannae L. (S. ravannae) using response surface methodology based on central composite design. Kinetic study of the biomass was conducted at four different heating rates of 10, 20, 40 and 60 °C min -1 and results were interpreted by Friedman, Kissinger Akira Sunnose and Flynn-Wall-Ozawa methods. Average activation energy 151.45 kJ mol -1 was used for evaluation of reaction mechanism following Criado master plot. Maximum bio-oil yield of 38.1 wt% was obtained at pyrolysis temperature of 550 °C, heating rate of 20 °C min -1 and nitrogen flow rate of 226 mL min -1 . Study on bio-oil quality revealed higher content of hydrocarbon, antioxidant property, total phenolic content and metal chelating capacity. These opened up probable applications of S. ravannae bio-oil in different fields including fuel, food industry and biomedical domain. Copyright © 2018 Elsevier Ltd. All rights reserved.

  20. A study of dose effect response and the interrelationship between cell survival, mutation and chromosomal aberration

    International Nuclear Information System (INIS)

    Chadwick, K.H.; Leenhouts, H.P.; Werry, P.A.Th.J.; Ramulu, K.S.

    1980-01-01

    Research was carried out on four topics: generation of cell reproductive death and mutation induction in cell cultures of Haplopappus gracilis by ionizing radiation; radiation induced repair in Saintpaulia epidermis cells; analysis of the combined effect of radiation with other mutagens; and the effect of very low doses of radiation on the Oenothera model system. (Auth.)

  1. Codominance of Acer saccharum and Fagus grandifolia: the role of Fagus root sprouts along a slope gradient in an old-growth forest.

    Science.gov (United States)

    Takahashi, Koichi; Arii, Ken; Lechowicz, Martin J

    2010-09-01

    We studied how the unusual capacity of mature Fagus grandifolia to form clumps of clonal stems from root sprouts can contribute to its frequent codominance with Acer saccharum in southern Quebec, Canada. In an old-growth forest, the degree of dominance by the two species shifted along topographic gradients spanning a few hundreds of meters, with Fagus more frequent on lower slopes and Acer on upper slopes. The frequency distribution of Fagus stem diameter had an inverse J distribution at all slope positions, which is indicative of continuous recruitment. Acer stem diameter also had an inverse J pattern, except at lower slope positions where size structure was discontinuous. For stems Acer were positively correlated with conspecific canopy trees only on the mid-slope. There were many Fagus seedlings around Acer canopy trees at the lower slope, suggesting the potential replacement of Acer canopy trees by Fagus. This study suggests that the regeneration traits of the two species changed with slope position and that Fagus patches originating from root sprouts can contribute to the maintenance of Acer-Fagus codominance at the scale of local landscapes.

  2. Identifying Quantitative Trait Loci (QTLs) and Developing Diagnostic Markers Linked to Orange Rust Resistance in Sugarcane (Saccharum spp.).

    Science.gov (United States)

    Yang, Xiping; Islam, Md S; Sood, Sushma; Maya, Stephanie; Hanson, Erik A; Comstock, Jack; Wang, Jianping

    2018-01-01

    Sugarcane ( Saccharum spp.) is an important economic crop, contributing up to 80% of table sugar used in the world and has become a promising feedstock for biofuel production. Sugarcane production has been threatened by many diseases, and fungicide applications for disease control have been opted out for sustainable agriculture. Orange rust is one of the major diseases impacting sugarcane production worldwide. Identifying quantitative trait loci (QTLs) and developing diagnostic markers are valuable for breeding programs to expedite release of superior sugarcane cultivars for disease control. In this study, an F 1 segregating population derived from a cross between two hybrid sugarcane clones, CP95-1039 and CP88-1762, was evaluated for orange rust resistance in replicated trails. Three QTLs controlling orange rust resistance in sugarcane (qORR109, qORR4 and qORR102) were identified for the first time ever, which can explain 58, 12 and 8% of the phenotypic variation, separately. We also characterized 1,574 sugarcane putative resistance ( R ) genes. These sugarcane putative R genes and simple sequence repeats in the QTL intervals were further used to develop diagnostic markers for marker-assisted selection of orange rust resistance. A PCR-based Resistance gene-derived maker, G1 was developed, which showed significant association with orange rust resistance. The putative QTLs and marker developed in this study can be effectively utilized in sugarcane breeding programs to facilitate the selection process, thus contributing to the sustainable agriculture for orange rust disease control.

  3. Cold Responsive Gene Expression Profiling of Sugarcane and Saccharum spontaneum with Functional Analysis of a Cold Inducible Saccharum Homolog of NOD26-Like Intrinsic Protein to Salt and Water Stress.

    Directory of Open Access Journals (Sweden)

    Jong-Won Park

    Full Text Available Transcriptome analysis of sugarcane hybrid CP72-1210 (cold susceptible and Saccharum spontaneum TUS05-05 (cold tolerant using Sugarcane Assembled Sequences (SAS from SUCEST-FUN Database showed that a total of 35,340 and 34,698 SAS genes, respectively, were expressed before and after chilling stress. The analysis revealed that more than 600 genes are differentially expressed in each genotype after chilling stress. Blast2Go annotation revealed that the major difference in gene expression profiles between CP72-1210 and TUS05-05 after chilling stress are present in the genes related to the transmembrane transporter activity. To further investigate the relevance of transmembrane transporter activity against abiotic stress tolerance, a S. spontaneum homolog of a NOD26-like major intrinsic protein gene (SspNIP2 was selected for functional analysis, of which expression was induced after chilling stress in the cold tolerant TUS05-05. Quantitative real-time PCR showed that SspNIP2 expression was increased ~2.5 fold at 30 minutes after cold treatment and stayed induced throughout the 24 hours of cold treatment. The amino acid sequence analysis of the cloned SspNIP2 confirmed the presence of six transmembrane domains and two NPA (Asn-Pro-Ala motifs, signature features of major intrinsic protein families. Amino acid analysis confirmed that four amino acids, comprising the ar/R (aromatic residue/arginine region responsible for the substrate specificity among MIPs, are conserved among monocot silicon transporters and SspNIP2. Salinity stress test on SspNIP2 transgenic tobacco plants resulted in more vigorous transgenic lines than the non-transgenic tobacco plants, suggesting some degree of tolerance to salt stress conferred by SspNIP2. SspNIP2-transgenic plants, exposed to 2 weeks of water stress without irrigation, developed various degrees of water stress symptom. The water stress test confirmed that the SspNIP2 transgenic lines had lower evapotranspiration

  4. Environmental Assessment for Construction of a Multi-Story Dormitory at Tyndall Air Force Base, Florida

    Science.gov (United States)

    2006-11-01

    ionantha T E Cypress domes White-flowered wild petunia Ruellia noctiflora E Wet prairie BIRDS American oystercatcher...The parking and street lights will be full cut fixtures in addition to being fully s hielded and using low pressure sodium lamps .. 2 ) The height...uction Ms .. Patrick, Thank you for the rapid response to our letter. Below are tbe r .esponses to your comments: 1 . The light wi l l be ful l cut

  5. Growing, regeneration and radiosensibility of sugarcane callus (Saccharum spp. hybrid var. “SP 70-1284” treated with gamma radiation source 60Co.

    Directory of Open Access Journals (Sweden)

    Apolonio Valdez Balero

    2004-07-01

    Full Text Available Calluses in growth of sugar cane, variety “SP 70-1284”, were radiated with a source of 60Co. The studied doses were between 10 and 80 Gy. The affectation of the Gamma radiation source 60Co in the growth of the callus and the regeneration of plants was evaluated. The results indicated that the dose that diminished the growth of callus in a value under 50% was the one of 30 Gy, and also the dose that diminished the regenerative capacity of the callus below to the 50% of the population was the one of 30 Gy. The results showed that for this variety of sugar cane doses superior to 30Gy of Gamma radiations jeopardizes the growth and the later regeneration of the callus. Its application is recommended to induce genetic variability in the variety of sugar cane “SP 70-1284”, as well as in programs of genetic improvement by mutations, in susceptible varieties of the sugar cane red blight (Puccinia melanocephala Syd using calluses in growth. Key words: callus, hybrid, in vitro, improvement, rust, Saccharum sp, variability

  6. Isolation of nucleotide binding site-leucine rich repeat and kinase resistance gene analogues from sugarcane (Saccharum spp.).

    Science.gov (United States)

    Glynn, Neil C; Comstock, Jack C; Sood, Sushma G; Dang, Phat M; Chaparro, Jose X

    2008-01-01

    Resistance gene analogues (RGAs) have been isolated from many crops and offer potential in breeding for disease resistance through marker-assisted selection, either as closely linked or as perfect markers. Many R-gene sequences contain kinase domains, and indeed kinase genes have been reported as being proximal to R-genes, making kinase analogues an additionally promising target. The first step towards utilizing RGAs as markers for disease resistance is isolation and characterization of the sequences. Sugarcane clone US01-1158 was identified as resistant to yellow leaf caused by the sugarcane yellow leaf virus (SCYLV) and moderately resistant to rust caused by Puccinia melanocephala Sydow & Sydow. Degenerate primers that had previously proved useful for isolating RGAs and kinase analogues in wheat and soybean were used to amplify DNA from sugarcane (Saccharum spp.) clone US-01-1158. Sequences generated from 1512 positive clones were assembled into 134 contigs of between two and 105 sequences. Comparison of the contig consensuses with the NCBI sequence database using BLASTx showed that 20 had sequence homology to nuclear binding site and leucine rich repeat (NBS-LRR) RGAs, and eight to kinase genes. Alignment of the deduced amino acid sequences with similar sequences from the NCBI database allowed the identification of several conserved domains. The alignment and resulting phenetic tree showed that many of the sequences had greater similarity to sequences from other species than to one another. The use of degenerate primers is a useful method for isolating novel sugarcane RGA and kinase gene analogues. Further studies are needed to evaluate the role of these genes in disease resistance.

  7. Effect of sugar industry spentwash (diluted) on the characteristics of soil and sugarcane (Saccharum officinarum L.) growth in the subtropical environment of Sindh, Pakistan.

    Science.gov (United States)

    Kaloi, Ghulam Muhiyuddin; Memon, Mehrunisa; Memon, Kazi Suleman; Ahmad, Sagheer; Sheikh, Saghir Ahmed; Jamro, Ghulam Murtaza

    2017-03-01

    Spentwash is a rich source of organic matter and essential plant nutrients in addition to excess salts. Sugar mills in Pakistan discharge about 3.48 million tons of spentwash annually, with no proper procedures for its disposal or utilization. To test the effect of diluted spentwash on soil and the soil's ability to support plant growth, sugarcane (Saccharum officinarum L.) variety CPF-237 was planted. The experiment was conducted in a randomized complete block design involving factorial combination of four concentrations of each spentwash (0, 10, 20, and 30%) and mineral fertilizers (0, 1/3, 2/3, and the full recommended rate of NP). The 10% spentwash plus 2/3 mineral fertilizer treatment substituted 33% each of N and P and 100% of K, saving mineral fertilizer cost (Rs. 48600 ∼ US$458) in addition to 29.54% increase in yield over full NP fertilizer. The same treatment also improved the soil organic matter (65%), N (20%), P (25%), and K (230%) over full NP treatment alone. An increase in the salt content of the soil was detected within the prescribed limits, with the exception of HCO 3 .

  8. Relationship between leaf optical properties, chlorophyll fluorescence and pigment changes in senescing Acer saccharum leaves.

    Science.gov (United States)

    Junker, Laura Verena; Ensminger, Ingo

    2016-06-01

    The ability of plants to sequester carbon is highly variable over the course of the year and reflects seasonal variation in photosynthetic efficiency. This seasonal variation is most prominent during autumn, when leaves of deciduous tree species such as sugar maple (Acer saccharum Marsh.) undergo senescence, which is associated with downregulation of photosynthesis and a change of leaf color. The remote sensing of leaf color by spectral reflectance measurements and digital repeat images is increasingly used to improve models of growing season length and seasonal variation in carbon sequestration. Vegetation indices derived from spectral reflectance measurements and digital repeat images might not adequately reflect photosynthetic efficiency of red-senescing tree species during autumn due to the changes in foliar pigment content associated with autumn phenology. In this study, we aimed to assess how effectively several widely used vegetation indices capture autumn phenology and reflect the changes in physiology and photosynthetic pigments during autumn. Chlorophyll fluorescence and pigment content of green, yellow, orange and red leaves were measured to represent leaf senescence during autumn and used as a reference to validate and compare vegetation indices derived from leaf-level spectral reflectance measurements and color analysis of digital images. Vegetation indices varied in their suitability to track the decrease of photosynthetic efficiency and chlorophyll content despite increasing anthocyanin content. Commonly used spectral reflectance indices such as the normalized difference vegetation index and photochemical reflectance index showed major constraints arising from a limited representation of gradual decreases in chlorophyll content and an influence of high foliar anthocyanin levels. The excess green index and green-red vegetation index were more suitable to assess the process of senescence. Similarly, digital image analysis revealed that vegetation

  9. Effect of Elevated Atmospheric CO2 and Temperature on Leaf Optical Properties and Chlorophyll Content in Acer saccharum (Marsh.)

    Science.gov (United States)

    Carter, Gregory A.; Bahadur, Raj; Norby, Richard J.

    1999-01-01

    Elevated atmospheric CO2 pressure and numerous causes of plant stress often result in decreased leaf chlorophyll contents and thus would be expected to alter leaf optical properties. Hypotheses that elevated carbon dioxide pressure and air temperature would alter leaf optical properties were tested for sugar maple (Acer saccharum Marsh.) in the middle of its fourth growing season under treatment. The saplings had been growing since 1994 in open-top chambers at Oak Ridge, Tennessee under the following treatments: 1) Ambient CO2 pressure and air temperature (control); 2) CO2 pressure approximately 30 Pa above ambient; 3) Air temperatures 3 C above ambient; 4) Elevated CO2 and air temperature. Spectral reflectance, transmittance, and absorptance in the visible spectrum (400-720 nm) did not change significantly (rho = 0.05) in response to any treatment compared with control values. Although reflectance, transmittance, and absorptance at 700 nm correlated strongly with leaf chlorophyll content, chlorophyll content was not altered significantly by the treatments. The lack of treatment effects on pigmentation explained the non-significant change in optical properties in the visible spectrum. Optical properties in the near-infrared (721-850 nm) were similarly unresponsive to treatment with the exception of an increased absorptance in leaves that developed under elevated air temperature alone. This response could not be explained by the data, but might have resulted from effects of air temperature on leaf internal structure. Results indicated no significant potential for detecting leaf optical responses to elevated CO2 or temperature by the remote sensing of reflected radiation in the 400-850 nm spectrum.

  10. Respons embriogenesis mikrospora tanaman tebu (Saccharum spp. pada suhu dan lama inkubasi cabang malai di dalam medium B

    Directory of Open Access Journals (Sweden)

    Suaib Suaib

    2012-02-01

    Full Text Available Microspore embryogenesis under different temperature and time duration pretreatments of spike segments of Saccharum spp., clonePS862 has been studied in in-vitro laboratory of Faculty of Biology Gadjah Mada University, Yogyakarta. Combination pretreatmentsof a 4 and 34 ° C of temperatures, and 0, 2, 4, and 7 days duration of incubation of spike segments in the B medium were studied inthis observation. A B5 medium of Gamborg supplemented with 2 mgl-1 2.4-D or 2 mg.l-1 NAA was also studied on the formation of proembryos and embryos under 25 ° C temperature in the dark for at least 30 days. Results of the research showed that the percentageof viable microspores was decreased as simultaneously prolonged of the time of duration in the B medium either under low temperatureor under high temperature pretreatments tested. Incubation of spike segments under low temperature after 4 days was decreased the percentage of viable microspores over 44%. In contrast, under high temperature pretreatment the percentage of viable microspores was decreased over 54%. The higher percentage (12.5% of binucleate microspores was achieved after 7 days incubation of spike segmentsin the B medium under the low temperature compared to the high temperature (7.14%. Besides, the higher percentage (87.5% of simmetrical binucleate microspores as pro embryo and embryo precursor was obtained after 2 days incubation in the B medium underthe low temperature compared to the high temperature (79.0%. Finally, only B5 medium supplemented with 2 mgl-1 of NAA was yielded the pro embryos and embryos after 30 days of incubation.

  11. Physiological and morphological effects of high water tables on early growth of giant reed (Arundo donax), elephant grass (Pennisetum purpureum), energycane and sugarcane (Saccharum spp.)

    Energy Technology Data Exchange (ETDEWEB)

    Jennewein, Stephen Peter [Univ. of Florida, Gainesville, FL (United States)

    2013-01-01

    Here, an increasing demand for renewable energy sources has spurred interest in high-biomass crops used for energy production. Species potentially well-suited for biofuel production in the seasonally wet organic Everglades Agricultural Area (EAA) of Florida include giant reed (Arundo donax), elephant grass (Pennisetum Purpureum), energycane (Saccharum spp.), and sugarcane (Saccharum spp.). The objectives in this study were to evaluate the role of fluctuating water tables on the morphology, physiology, and early season growth of these four genotypes. The candidate genotypes were grown in a greenhouse under three water table depths, defined by distance of the water table from the soil surface: two constant water tables (-16 cm and -40 cm) along with a flood cycle (2 weeks of flood to the soil level followed by 2 weeks at -40 cm from the soil level). The genotypes included CP 89-2143 (sugarcane), L 79-1002 (energycane), Merkeron (elephant grass), and wild type (giant reed). The experiment was repeated for plant cane, first ratoon, and successive plant cane crop cycles. Reductions in dry matter yield were observed among genotypes subjected to the -40 cm drained, periodically flooded (40F) water table relative to the -40 cm constant (40C) or -16 cm constant (16C). Plant cane dry weights were reduced by 37% in giant reed, 52% in elephant grass, 42% in energycane, and 34% in sugarcane in the 40F compared to 40C water table treatments. Similarly, in the first ratoon crop dry weights were reduced by 29% in giant reed, 42% in elephant grass, 27% in energycane, and 62% in sugarcane. In plant cane and successive plant cane, average total dry weight was greatest for elephant grass whereas ratoon total dry weight was greatest for energycane. Genotype had more pronounced effects on physiological attributes than water table including the highest stomatal conductance and SPAD values in giant reed, and the highest stalk populations in elephant grass and

  12. Optimization of ultrasonic circulating extraction of samara oil from Acer saccharum using combination of Plackett-Burman design and Box-Behnken design.

    Science.gov (United States)

    Chen, Fengli; Zhang, Qiang; Fei, Shimin; Gu, Huiyan; Yang, Lei

    2017-03-01

    In this study, ultrasonic circulating extraction (UCE) technique was firstly and successfully applied for extraction of samara oil from Acer saccharum. The extraction kinetics were fitted and described, and the extraction mechanism was discussed. Through comparison, n-hexane was selected as the extraction solvent, the influence of solvent type on the responses was detailedly interpreted based on the influence of their properties on the occurrence and intensity of cavitation. Seven parameters potentially influencing the extraction yield of samara oil and content of nervonic acid, including ultrasound irradiation time, ultrasound irradiation power, ultrasound temperature, liquid-solid ratio, soaking time, particle size and stirring rate, were screened through Plackett-Burman design to determine the significant variables. Then, three parameters performed statistically significant, including liquid-solid ratio, ultrasound irradiation time and ultrasound irradiation power, were further optimized using Box-Behnken design to predict optimum extraction conditions. Satisfactory yield of samara oil (11.72±0.38%) and content of nervonic acid (5.28±0.18%) were achieved using the optimal conditions. 1% proportion of ethanol in extraction solvent, 120°C of drying temperature and 6.4% moisture were selected and applied for effective extraction. There were no distinct differences in the physicochemical properties of samara oil obtained by UCE and Soxhlet extraction, and the samara oil obtained by UCE exhibited better antioxidant activities. Therefore, UCE method has enormous potential for efficient extraction of edible oil with high quality from plant materials. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Influence of northern limit range on genetic diversity and structure in a widespread North American tree, sugar maple (Acer saccharum Marshall).

    Science.gov (United States)

    Graignic, Noémie; Tremblay, Francine; Bergeron, Yves

    2018-03-01

    Due to climate change, the ranges of many North American tree species are expected to shift northward. Sugar maple ( Acer saccharum Marshall) reaches its northern continuous distributional limit in northeastern North America at the transition between boreal mixed-wood and temperate deciduous forests. We hypothesized that marginal fragmented northern populations from the boreal mixed wood would have a distinct pattern of genetic structure and diversity. We analyzed variation at 18 microsatellite loci from 23 populations distributed along three latitudinal transects (west, central, and east) that encompass the continuous-discontinuous species range. Each transect was divided into two zones, continuous (temperate deciduous) and discontinuous (boreal mixed wood), based on sugar maple stand abundance. Respective positive and negative relationships were found between the distance of each population to the northern limit (D_north), and allelic richness ( A R ) and population differentiation ( F ST ). These relations were tested for each transect separately; the pattern (discontinuous-continuous) remained significant only for the western transect. structure analysis revealed the presence of four clusters. The most northern populations of each transect were assigned to a distinct group. Asymmetrical gene flow occurred from the southern into the four northernmost populations. Southern populations in Québec may have originated from two different postglacial migration routes. No evidence was found to validate the hypothesis that northern populations were remnants of a larger population that had migrated further north of the species range after the retreat of the ice sheet. The northernmost sugar maple populations possibly originated from long-distance dispersal.

  14. Effect of iso-osmotic salt and water stress in relation to adjustment on mutant sugarcane (Saccharum officinarum L.) plant lines

    International Nuclear Information System (INIS)

    Ahuja, Akash V.; Kalwade, Sachin B.; Nikam, Ashok A.; Devarumath, R.M.; Chauvan, Viraj S.; Kanse, Sangram S.

    2014-01-01

    Gamma radiation induced mutagenesis followed by in vitro selection was employed for salt tolerance in popular sugarcane (Saccharum officinarum L.) cv.CoM0265. Assimilated regenerated mutant plantlets were planted on control as well as salt affected soil. Mutants which showed relatively good response with respect to its quantitative and qualitative parameters were selected for priming experiment. Nine mutants and its respective control and parent control which are known to vary in salt tolerance under field conditions were studied. In order to discriminate between the ionic and osmotic components of salt stress, mutant plants were treated with NaCl salt (100 mM) or polyethylene glycol-PEG 8000 solutions (20%) for 10 days. Both NaCI and PEG treatment significantly reduced leaf width, number of green leaves and chlorophyll stability index. Osmotic adjustment indicated that the NaCI and PEG stress lead to accumulation of osmolytes, however sugar level changes non significantly. The ion concentration was drastically affected upon NaCI treatment, whereas PEG stress accumulated relatively less amount of Na + ions in comparison to NaCl. However, there was an increase in K + concentration upon PEG treatment, whereas NaCI stress accumulated less K + concentration with respect to PEG and control. The NaCI and PEG treated mutant plants showed increased activities of superoxide dismutase (SOD) and Catalase (CAT) in comparison to its control and parent control. Among the mutant selected gamma rays irradiation in corporation with enhanced tolerance to abiotic stress is one of the important goals for the biotechnological improvement of crop plants. Enhanced salinity tolerance may prove beneficial to improve the competitiveness of the popular sugarcane cultivars and their commercial cultivation in saline areas. (author)

  15. The reproductive biology of Saccharum spontaneum L.: implications for management of this invasive weed in Panama

    Directory of Open Access Journals (Sweden)

    Graham Bonnett

    2014-01-01

    Full Text Available Saccharum spontaneum L. is an invasive grass that has spread extensively in disturbed areas throughout the Panama Canal watershed (PCW, where it has created a fire hazard and inhibited reforestation efforts. Currently physical removal of aboveground biomass is the primary means of controlling this weed, which is largely ineffective and does little to inhibit spread of the species. Little is known about reproduction of this species, although it is both rhizomatous and produces abundant seed. Here we report a series of studies looking at some of the basic reproductive mechanisms and strategies utilised by S. spontaneum to provide information to support development of better targeted management strategies.We found that seed produced between September and November was germinable both in the lab and in situ. Genetic diversity of mature stands was assessed using microsatellite markers and found to be high, even at small scales. Studies of vegetative reproduction showed that buds on stems that had been dried for up to six weeks were still capable of sprouting. Separate experiments showed that stem fragments could sprout when left on the surface or buried shallowly and that larger pieces sprouted more readily than smaller pieces.Collectively these results demonstrate that S. spontaneum in the PCW has the capability to produce many propagules that can successfully recruit and it is likely that seed dispersal drives the spread of the species. Timing of management actions to reduce flowering would significantly reduce the seed load into the environment and help to prevent spread to new sites. Similarly, where biomass is cut, cutting stems into smaller pieces will allow the stems to dry out and reduce the ability of buds to sprout. Additionally, attention should be paid to prevent accidental transport to new sites on machinery.

  16. saccharum saccharum officinarum officinarum ) bagasse and parkia

    African Journals Online (AJOL)

    eobe

    in wastewater include: suspended solids, biodegradable organic ... The seeds of the plant are enclosed in a yellowish, mealy, sweet ... and parkia biglobosa in wastewater treatment involve ..... thesis, Graduate Faculty of the Louissiana State.

  17. High polymorphism in Est-SSR loci for cellulose synthase and β-amylase of sugarcane varieties (Saccharum spp.) used by the industrial sector for ethanol production.

    Science.gov (United States)

    Augusto, Raphael; Maranho, Rone Charles; Mangolin, Claudete Aparecida; Pires da Silva Machado, Maria de Fátima

    2015-01-01

    High and low polymorphisms in simple sequence repeats of expressed sequence tag (EST-SSR) for specific proteins and enzymes, such as β-amylase, cellulose synthase, xyloglucan endotransglucosylase, fructose 1,6-bisphosphate aldolase, and fructose 1,6-bisphosphatase, were used to illustrate the genetic divergence within and between varieties of sugarcane (Saccharum spp.) and to guide the technological paths to optimize ethanol production from lignocellulose biomass. The varieties RB72454, RB867515, RB92579, and SP813250 on the second stage of cutting, all grown in the state of Paraná (PR), and the varieties RB92579 and SP813250 cultured in the PR state and in Northeastern Brazil, state of Pernambuco (PE), were analyzed using five EST-SSR primers for EstC66, EstC67, EstC68, EstC69, and EstC91 loci. Genetic divergence was evident in the EstC67 and EstC69 loci for β-amylase and cellulose synthase, respectively, among the four sugarcane varieties. An extremely high level of genetic differentiation was also detected in the EstC67 locus from the RB82579 and SP813250 varieties cultured in the PR and PE states. High polymorphism in SSR of the cellulose synthase locus may explain the high variability of substrates used in pretreatment and enzymatic hydrolysis processes, which has been an obstacle to effective industrial adaptations.

  18. Agar alternatives for micropropagation of African violet ( Saintpaulia ...

    African Journals Online (AJOL)

    Agar is one of the most popular solidifying agents in plant tissue culture. High price of pure grade agar and fear of over exploitation of its resources caused searching for low cost alternatives. In this study, liquid medium with cotton substratum and different combinations of starch, semolina, potato powder and agar in two ...

  19. Application of atomic energy in agriculture. Annual report, 1975

    Energy Technology Data Exchange (ETDEWEB)

    1976-01-01

    Activities over the year 1975 are reviewed in the annual report for the entire research programme comprising: primary radiation effects in inert and biological material, dose fractionation effect in Saintpaulia, adventitious bud technique in mutation breeding, mutation research in potatoes, protein improvements in peas and maize, disease resistance in tomatoes, pepper, wheat and barley, food preservation, radiation genetics of insect pests, genetics of higher plants, soil-plant studies, element behaviour in the soil and water environment, heavy metals in plants and soils, development of methods and instruments.

  20. Application of atomic energy in agriculture (Annual Report 1974)

    International Nuclear Information System (INIS)

    1975-01-01

    Activities and results over the year 1974 are reviewed in the annual report for the entire research programme comprising: lyoluminescence, perspex dosemeters, primary radiation effects in biological materials, dose fractionation effect in Saintpaulia, adventitious bud technique in mutation breeding, mutation research in potatoes, protein improvement in peas, disease resistance in tomatoes, wheat, peas and barley, food preservation, genetic pest control, genetics of higher plants, soil-plant studies, element behaviour in soils and groundwater, heavy metals in plants, liquid waste reuse after irradiation, development of methods and instruments

  1. Application of atomic energy in agriculture, annual report 1975

    International Nuclear Information System (INIS)

    1976-01-01

    Activities over the year 1975 are reviewed in the annual report for the entire research programme comprising: primary radiation effects in inert and biological material, dose fractionation effect in Saintpaulia, adventitious bud technique in mutation breeding, mutation research in potatoes, protein improvements in peas and maize, disease resistance in tomatoes, pepper, wheat and barley, food preservation, radiation genetics of insect pests, genetics of higher plants, soil-plant studies, element behaviour in the soil and water environment, heavy metals in plants and soils, development of methods and instruments

  2. Simulated root dynamics of a 160-year-old sugar maple (Acer saccharum Marsh.) tree with and without ozone exposure using the TREGRO model.

    Science.gov (United States)

    Retzlaff, W. A.; Weinstein, D. A.; Laurence, J. A.; Gollands, B.

    1996-01-01

    Because of difficulties in directly assessing root responses of mature forest trees exposed to atmospheric pollutants, we have used the model TREGRO to analyze the effects of a 3- and a 10-year exposure to ozone (O(3)) on root dynamics of a simulated 160-year-old sugar maple (Acer saccharum Marsh.) tree. We used existing phenological, allometric, and growth data to parameterize TREGRO to produce a simulated 160-year-old tree. Simulations were based on literature values for sugar maple fine root production and senescence and the photosynthetic responses of sugar maple seedlings exposed to O(3) in open-top chambers. In the simulated 3-year exposure to O(3), 2 x ambient atmospheric O(3) concentrations reduced net carbon (C) gain of the 160-year-old tree. This reduction occurred in the C storage pools (total nonstructural carbohydrate, TNC), with most of the reduction occurring in coarse (woody) roots. Total fine root production and senescence were unaffected by the simulated 3-year exposure to O(3). However, extending the simulated O(3) exposure period to 10 years depleted the TNC pools of the coarse roots and reduced total fine root production. Similar reductions in TNC pools have been observed in forest-grown sugar maple trees exhibiting symptoms of stress. We conclude that modeling can aid in evaluating the belowground response of mature forest trees to atmospheric pollution stress and could indicate the potential for gradual deterioration of tree health under conditions of long-term stress, a situation similar to that underlying the decline of sugar maple trees.

  3. Nutrients, Antioxidant Capacity and Safety of Hot Water Extract from Sugar Maple (Acer saccharum M.) and Red Maple (Acer rubrum L.) Bark.

    Science.gov (United States)

    Bhatta, Sagar; Ratti, Cristina; Poubelle, Patrice E; Stevanovic, Tatjana

    2018-03-01

    Sugar maple (Acer saccharum M.) and red maple (Acer rubrum L.) barks were treated with hot water to extract nutrients in order to explore, for the first time, its potential as safe dietary antioxidants. The organic and inorganic nutrients of these extracts, as well as their safety on human PLB-985 cells differentiated into neutrophils-like cells, were determined. Proximate analysis showed that both bark extracts were low in moisture and fat. Sugar maple bark extract (SM-BX) showed crude protein and ash content higher than those found in red maple bark extract (RM-BX). In addition, SM-BX had total sugars higher than those evaluated in RM-BX, while complex sugars (oligo- and/or poly-saccharides) were similarly abundant in both bark extracts. Furthermore, SM-BX demonstrated a wide array of vital minerals (K, Ca, Mg, P, Na, Fe and Cu) in quantity larger than that evaluated in RM-BX, whereas RM-BX have Zn and Mn levels higher than those found in SM-BX. Phytochemical analyses showed that RM-BX exhibited total phenolic and flavonoid contents higher than those measured in SM-BX. Consequently, RM-BX presented an antioxidant activity higher than that of SM-BX: 2.85-fold ABTS radical cation scavenging capacity and 1.9-fold oxygen radical absorbance capacity. Finally, RM-BX and SM-BX were greatly safe since, at concentration up to 100 μg/ml, they did not modify the viability of neutrophils as determined by flow-cytometry assay using Annexin V-FITC/Propidum Iodide as markers. In conclusion, our in vitro studies indicate that both red and sugar maple bark extracts have a real potential as food additives.

  4. Genetic variation in transpiration efficiency and relationships between whole plant and leaf gas exchange measurements in Saccharum spp. and related germplasm.

    Science.gov (United States)

    Jackson, Phillip; Basnayake, Jaya; Inman-Bamber, Geoff; Lakshmanan, Prakash; Natarajan, Sijesh; Stokes, Chris

    2016-02-01

    Fifty-one genotypes of sugarcane (Saccharum spp.) or closely related germplasm were evaluated in a pot experiment to examine genetic variation in transpiration efficiency. Significant variation in whole plant transpiration efficiency was observed, with the difference between lowest and highest genotypes being about 40% of the mean. Leaf gas exchange measurements were made across a wide range of conditions. There was significant genetic variation in intrinsic transpiration efficiency at a leaf level as measured by leaf internal CO2 (Ci) levels. Significant genetic variation in Ci was also observed within subsets of data representing narrow ranges of stomatal conductance. Ci had a low broad sense heritability (Hb = 0.11) on the basis of single measurements made at particular dates, because of high error variation and genotype × date interaction, but broad sense heritability for mean Ci across all dates was high (Hb = 0.81) because of the large number of measurements taken at different dates. Ci levels among genotypes at mid-range levels of conductance had a strong genetic correlation (-0.92 ± 0.30) with whole plant transpiration efficiency but genetic correlations between Ci and whole plant transpiration efficiency were weaker or not significant at higher and lower levels of conductance. Reduced Ci levels at any given level of conductance may result in improved yields in water-limited environments without trade-offs in rates of water use and growth. Targeted selection and improvement of lowered Ci per unit conductance via breeding may provide longer-term benefits for water-limited environments but the challenge will be to identify a low-cost screening methodology. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  5. Comportamiento productivo y fitosanitario de la nueva variedad de caña de azúcar (Saccharum spp. TUC 95-37 en Tucumán, R. Argentina Productivity and disease tolerance of TUC 95-37, a new sugarcane (Saccharum spp. variety in Tucumán, Argentina

    Directory of Open Access Journals (Sweden)

    Ernesto R. Chavanne

    2012-06-01

    Full Text Available En este trabajo se presenta el comportamiento productivo y fitosanitario del nuevo cultivar de caña de azúcar (Saccharum spp. TUC 95-37, obtenido y seleccionado por el Programa de Mejoramiento Genético de la Caña de Azúcar de la Estación Experimental Agroindustrial Obispo Colombres (Tucumán, R. Argentina. La información que se presenta proviene de 24 cosechas realizadas en ensayos con 18 genotipos, implantados de acuerdo a un diseño experimental de bloques completamente aleatorizados con tres repeticiones. Los ensayos se replicaron en seis localidades de Tucumán y fueron evaluados en cuatro edades de corte (caña planta, soca 1, soca 2 y soca 3 entre los años 2002 y 2005. Las variables toneladas de caña/ha y de azúcar/ha y rendimiento fabril porciento se evaluaron en mayo. Para cada variable, edad y localidad se realizaron análisis de la varianza y pruebas de comparación de medias. También se aplicó la metodología de GGE biplot como herramienta gráfica, para identificar genotipos superiores y estables frente a distintos ambientes de expresión. Se evaluó además el comportamiento fitosanitario de TUC 95-37. Los resultados indicaron que la nueva variedad evidenció un comportamiento productivo similar al de LCP 85-384 en la mayoría de las edades de corte y localidades evaluadas. En caña planta, el nuevo cultivar fue el genotipo más productivo, presentando además una amplia adaptación a diferentes ambientes. En soca 1 y 2, TUC 95-37 resultó segunda en orden de mérito, siendo estable en las diferentes localidades. En soca 3, la nueva variedad evidenció una menor estabilidad frente al ambiente. Con respecto al comportamiento fitosanitario, TUC 95-37 se comportó como moderadasmente resistente a roya marrón (Puccinia melanocephala, a escaldadura de la hoja (Xanthomonas albilineans y a carbón (Sporisorium scitamineum. Se mostró moderadamente susceptible al mosaico (Sugarcane mosaic virus y a estría roja (Acidovorax

  6. Genet mosai tic vari ic virus iability s in Pa y in coa akistan s at prot n ...

    African Journals Online (AJOL)

    SAM

    Saccharum hybrid cultivar. Punjab, Pakistan. KC200152. This study ... Saccharum hybrid cultivar BO 72. Bihar, India. DQ842502 ...... Fan ZF geno. Chin. Farrag. Lesi. 48(1. Frenke. War term virus. Frenke poty regio. Frenke sequ mos. 2783. Garcia plan. Gemec. Lers prot from. Gopal. (Sac. Gopal suga. 7:11. Gotz R. Coc. 147(.

  7. TERMINAÇÃO DE BOVINOS BRANGUS INTEIROS E CASTRADOS COM CANA-DEAÇÚCAR (SACCHARUM OFFICINARUM OU SILAGEM DE CAPIM MOMBAÇA (PANICUM MAXIMUM CV MOMBAÇA

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    Marcelo Diniz dos Santos

    2014-06-01

    Full Text Available The aim of this study was to evaluate the intake, weight gain, feed:gain ratio, carcass quality and economic viability of using fresh sugar cane and mombaça grass silage in Brangus castrated and intact males on termination. We used 140 Brangus cattle, with an average of 21 months and 355 kg live weight, identified and randomly assigned to treatments: T1, 37 intac males and T2, 37 castrated animals receiving both fresh sugar cane (Saccharum officinarum crushed;T3, 33 intact males and T4, 33 castrated animals, both fed mombaça grass silage (Panicum maximum cv mombaça. For economic evaluation, it was estimated the production costs of bulky coming from the farm costs, expenses concentrates, diesel, hand labor, equipment maintenance and control of 46 Colloquium Agrariae, v. 10, n.1, Jan-Jun. 2014, p.45-54. DOI: 10.5747/ca.2014.v10.n1.a099 parasites were also considered. The final weight of the entire animal was higher (P <0.05 than castrated animals. And feed conversion was higher in castrated animals, indicating that the bulls were more efficient in converting food into weight gain. The higher net revenue observed in intact males (mombaça is justified by the greater weight gain and lower diet costs. Animals that received diet with mombaça grass silage had greater economic feasibility of that received diet with sugar cane.

  8. USO DEL BAGAZO ENRIQUECIDO CON EL HONGO Pleurotus ostreatus, EN DIETAS PARA BOVINOS ESTABULADOS EN CEBA UTILIZAÇÃO DO BAGAÇO ENRIQUECIDO COMO FUNGO Pleurotus ostreatus, EM DIETAS PARA BOVINOS ESTABULADOS EM ENGORDA Pleurotus ostreatus ENRICHED SUGAR CANEHUSKS UTILIZATION ON INDOOR CATTLEFATTENING DIETS

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    NATALIA LIZETTE CASTAÑO

    2012-12-01

    Full Text Available En la elaboración de la panela, entre el 40 y 54% es bagazo, el cual se caracteriza portener baja proteína y energía, altos compuestos lignocelulósicos, acompañado de una baja digestibilidad, por ello, tradicionalmente ha sido utilizado como combustiblepara las hornillas. El presente trabajo, evaluó el uso del bagazo enriquecido con el hongo Pleurotus ostreatus, como suplemento en dietas para bovinos, frente a otros tratamientos con y sin suplementación comercial. A todos los animales se les suministró una dieta balanceada que consistía en 18 Kg de pasto king grass (Saccharum sinense, 6 Kg de caña (Saccharum officinarum, 3 Kg de cogollo de caña (Saccharum officinarum, 3 Kg de gallinaza y 0,6 Kg de miel de panela, y suplemento ofrecido ad libitum a los tratamientos que lo requerían. Se analizaron las variables ganancia diaria de peso, conversión alimenticia, consumo de materiaseca y el efecto costo beneficio de la suplementación. No se presentaron diferencias significativas (PNa elaboração da rapadura, entre o 40% e 54% é bagaço, o qual se caracteriza por ter baixa proteína e energia, alto composto lignocelulósicos, acompanhado por uma baixa digestibilidade, portanto, tradicionalmente tem sido usado como combustível para os queimadores. Este trabalho avaliou o uso do bagaço enriquecido com o fungo Pleurotus ostreatus como suplemento em dietas para bovinos, comparado com outros tratamentos com e sem suplementação comercial. Aos animais todos foi subministrado uma dieta equilibrada que consistia de 18 Kg de grama king grass (Saccharum sinense, 6 Kg de cana (Saccharum officinarum, 3 Kg de broto de cana (Saccharum officinarum, 3 Kg de estrume e 0,6 Kg de mel de rapadura e suplemento oferecido ad libitum aos tratamentos que o requeiram. Analisaram-se as variáveis: ganho diário de peso, conversão alimentar e consumo de matéria seca e o efeito de custo benefício da suplementação. Não apresentaram diferen

  9. Saccharum officinarum L.

    African Journals Online (AJOL)

    SARAH

    Rands et Abbott,. 1964) et à la mosaïque (Abbott, 1961), deux maladies dues à un virus, ainsi qu'à la gommose. (Hughes, 1964) provoquée par une bactérie. En réponse à cette contrainte, des hybrides interspécifiques entre S.

  10. Stem juice production of the C4 sugarcane (Saccharum officinarum) is enhanced by growth at double-ambient CO2 and high temperature.

    Science.gov (United States)

    Vu, Joseph C V; Allen, Leon H

    2009-07-15

    Two cultivars of sugarcane (Saccharum officinarum cv. CP73-1547 and CP88-1508) were grown for 3 months in paired-companion, temperature-gradient, sunlit greenhouses under daytime [CO2] of 360 (ambient) and 720 (double ambient) micromol mol(-1) and at temperatures of 1.5 degrees C (near ambient) and 6.0 degrees C higher than outside ambient temperature. Leaf area and biomass, stem biomass and juice and CO2 exchange rate (CER) and activities of ribulose bisphosphate carboxylase-oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) of fully developed leaves were measured at harvest. On a main stem basis, leaf area, leaf dry weight, stem dry weight and stem juice volume were increased by growth at doubled [CO2] or high temperature. Such increases were even greater under combination of doubled [CO2]/high temperature. Plants grown at doubled [CO2]/high temperature combination averaged 50%, 26%, 84% and 124% greater in leaf area, leaf dry weight, stem dry weight and stem juice volume, respectively, compared with plants grown at ambient [CO2]/near-ambient temperature combination. In addition, plants grown at doubled [CO2]/high temperature combination were 2-3-fold higher in stem soluble solids than those at ambient [CO2]/near-ambient temperature combination. Although midday CER of fully developed leaves was not affected by doubled [CO2] or high temperature, plants grown at doubled [CO2] were 41-43% less in leaf stomatal conductance and 69-79% greater in leaf water-use efficiency, compared with plants grown at ambient [CO2]. Activity of PEPC was down-regulated 23-32% at doubled [CO2], while high temperature did not have a significant impact on this enzyme. Activity of Rubisco was not affected by growth at doubled [CO2], but was reduced 15-28% at high temperature. The increases in stem juice production and stem juice soluble solids concentration for sugarcane grown at doubled [CO2] or high temperature, or at doubled [CO2]/high temperature combination, were partially

  11. EVALUACIÓN DE DOS MÉTODOS DE DIGESTIÓN ÁCIDA EN EL ANÁLISIS DE TEJIDO FOLIAR DE CAÑA (Saccharum officinarum L.

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    Betty Mendoza

    2015-02-01

    Full Text Available El desempeño de los métodos de digestión, vía seca (DVS y ácida asistida por microondas (DAAM fue evaluado en el análisis de muestras foliares de caña de azúcar (Saccharum officinarum L.. También se evaluó el efecto de las técnicas de detección comúnmente utilizadas en el laboratorio [P: espectrometría de absorción molecular visible (UV-VIS; K: espectrometría de emisión atómica (AES; Ca, Mg, Cu, Zn: espectrofotometría de absorción atómica (FAAS] respecto a la técnica de fluorescencia de rayos X de reflexión total (TXRF sobre los resultados obtenidos a partir de los dos métodos de digestión. La exactitud y precisión de los métodos de digestión se determinó con dos muestras certificadas, Tejido vegetal N°6 y N°11 (Comisión de Normalización y Acreditación - Sociedad Chilena de la Ciencia del Suelo. La determinación de P vía TXRF reportó resultados mayores respecto a UV-VIS en las muestras foliares de caña de azúcar. Los métodos de digestión mostraron resultados exactos y precisos en Ca cuando se utiliza FAAS o TXRF. En Zn y Cu se presentan desviaciones y varianzas asociadas a las concentraciones bajas en las muestras y su relación con el blanco de reactivos. En general, la precisión y exactitud están relacionadas principalmente con el rango de concentración de cada elemento en las muestras y el límite de cuantificación. No se encontro diferencias apreciables en cuanto al desempeño analítico de ambos métodos de digestión. La detección por TXRF presenta menor eficiencia analítica en cuanto a exactitud y precisión en algunos elementos, pero menores costos en tiempo de análisis.

  12. Contrasting nutritional acclimation of sugar maple (Acer saccharum Marsh. and red maple (Acer rubrum L. to increasing conifers and soil acidity as demonstrated by foliar nutrient balances

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    Alexandre Collin

    2016-07-01

    Full Text Available Sugar maple (Acer saccharum Marshall, SM is believed to be more sensitive to acidic and nutrient-poor soils associated with conifer-dominated stands than red maple (Acer rubrum L., RM. Greater foliar nutrient use efficiency (FNUE of RM is likely the cause for this difference. In the context of climate change, this greater FNUE could be key in favouring northward migration of RM over SM. We used the concept of foliar nutrient balances to study the nutrition of SM and RM seedlings along an increasing gradient in forest floor acidity conditioned by increasing proportions of conifers (pH values ranging from 4.39 under hardwoods, to 4.29 under mixed hardwood-conifer stands and 4.05 under conifer-dominated stands. Nutrients were subjected to isometric log-ratio (ilr transformation, which views the leaf as one closed system and considers interactions between nutrients. The ilr method eliminates numerical biases and weak statistical inferences based on raw or operationally’’ log-transformed data. We analyzed foliar nutrients of SM and RM seedlings and found that the [Ca,Mg,K|P,N] and [Ca,Mg|K] balances of SM seedlings were significantly different among soil acidity levels, whereas they did not vary for RM seedlings. For SM seedlings, these differences among soil acidity levels were due to a significant decrease in foliar Ca and Mg concentrations with increasing forest floor acidity. Similar differences in foliar balances were also found between healthy and declining SM stands estimated from literature values. Conversely, foliar balances of RM seedlings did not differ among soil acidity levels, even though untransformed foliar nutrient concentrations were significantly different. This result highlights the importance of using ilr transformation, since it provides more sensitive results than standard testing of untransformed nutrient concentrations. The lower nutrient requirements of RM and its greater capacity to maintain nutrient equilibrium are

  13. SUGARCANE (SACCHARUM SPP. HYBRIDS)

    African Journals Online (AJOL)

    DR. AMINU

    2010-06-01

    meagher, 2001). In Nigeria, total consumption of sugar was put in 2000 as. 700,000 tonnes per annum and growing at a rate ... RESULTS AND DISCUSSION. The explants cultured without 2,4-D turned brown and died within a few ...

  14. Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew. ex grassl

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    Claudia T. Guimarães

    1999-03-01

    Full Text Available Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum `LA Purple' and its proposed progenitor species (S. robustum `Mol 5829'. Various types (arbitrarily primed-PCR, RFLPs, and AFLPs of single-dose DNA markers (SDMs were used to construct genetic linkage maps for both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1,881 cM, while the Mol 5829 map contained 301 SDMs, spanning 65 linkage groups and 1,189 cM. Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae.Uma progênie de 100 indivíduos F1 obtidos de um cruzamento entre cana-de-açúcar (S. officinarum `LA Purple' e seu suposto progenitor (S. robustum `Mol 5829' foi analisada utilizando marcadores moleculares em dose única. Marcadores do tipo AP-PCR, RFLP e AFLP, gerando um total de 642 polimorfismos, foram mapeados em ambas espécies. O mapa genético de LA Purple foi composto de 341 marcadores, distribuídos em 74 grupos de ligação e 1.881 cM, enquanto que o mapa de ligação de Mol 5829 continha 301 marcadores ao longo de 65 grupos de ligação e 1.189 cM. A transmissão genética nessas duas espécies apresentou polissomia incompleta devido a detecção de 15% dos marcadores em dose simples ligados em fase de repulsão e 13% desses em Mol 5829. Devido a essa polissomia incompleta, os marcadores em dose múltipla não puderam ser mapeados por falta de um modelo genético para descrever tal segregação. O mapeamento de sondas de RFLP, conservadas entre espécies próximas evolutivamente, permitirá que os mapas genéticos gerados sejam utilizados como poderosas ferramentas no melhoramento e em estudos de ecologia, evolução e biologia molecular dentro das Andropogoneas.

  15. Use of AFLP marker system on sugarcane somaclones to study their resistance to rust

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    María Ileana Oloriz

    2002-10-01

    Full Text Available AFLPs (amplified fragment length polymorphism was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca: MseI/atg and EcoRI/aca: MseI/agg. Six polymorphic bands were obtained, two of these only appeared in the resistant genotypes, which are probably DNA sequences, related to rust resistance locus. These fragments have been cloned to study their nucleotide sequence and to investigate their roll in resistance mechanism develop by these mutants during Puccinia melanocephala infection. Key words: molecular markers, Puccinia. melanocephala, Saccharum

  16. Emergência e desenvolvimento de guanxuma (Sida rhombifolia, capim-braquiária (Brachiaria decumbens e cana-de-açúcar (Saccharum spp. influenciados por subprodutos da destilação do álcool Emergence and growth of arrowleaf sida (Sida rhombifolia, brachiaria grass (Brachiaria decumbens and sugarcane (Saccharum spp. as influenced by alcohol by-products

    Directory of Open Access Journals (Sweden)

    A.A.P.M. Azania

    2004-09-01

    Full Text Available Este trabalho objetivou avaliar os efeitos da aplicação de óleo de fúsel, comparativamente a vinhaça e flegmaça, sobre o desenvolvimento e a composição química de plantas de guanxuma (Sida rhombifolia, capim-braquiária (Brachiaria decumbens e cana-de-açúcar (variedade RB72454, cultivadas simultaneamente em casa de vegetação. As concentrações de 12,5; 25,0; 50,0; e 100,0% (v/v de cada subproduto e a testemunha (água foram aplicadas (numa taxa equivalente a 150 m³ ha-1 no solo dos vasos (22 L, contendo uma planta de cana-de-açúcar (13 cm de altura e 100 sementes de cada planta daninha. O delineamento foi o inteiramente casualizado, com 13 tratamentos e 4 repetições, em esquema fatorial 3 x 4 (três tipos de resíduos e quatro concentrações, e uma testemunha adicional com água. O óleo de fúsel inibiu a emergência de Sida rhombifolia e Brachiaria decumbens e matou a cana-de-açúcar. A vinhaça e a flegmaça prejudicaram a emergência e o desenvolvimento de B. decumbens, bem como o de S. rhombifolia, mas não o da cana-de-açúcar.This research aimed to compare the effects of fusel oil application to those of vinasse and flegmass application on the growth and chemical composition of arrowleaf sida plants (Sida rhombifolia, brachiaria grass (Brachiaria decumbens and sugarcane (Saccharum spp., cultivated simultaneously under greenhouse conditions. Concentrations of 12.5,25.0,50.0 and 100.0 % (v/v of each by-product and the check treatment (water were applied (rate equivalent to 150 m³ ha-1 to soil on 22 L-pots, containing one sugar-cane plant (13 cm of height, and 100 seeds of each weed. The experimental design was completely randomized , with 13 treatments and 4 repetitions, in a 3x4 factorial scheme (3 by-products and 4 concentrations and an additional check treatment with water. The fusel oil inhibited Sida rhombifolia and Brachiaria decumbens emergence with no emergence being verified for sugarcane. The vinasse and

  17. Caracterización espectroscópica del D-003 obtenido de la cera de caña de azúcar (Saccharum officinarum L.

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    David Marrero Delange

    Full Text Available Introducción: el D-003 es un ingrediente farmacéutico activo purificado a partir de la cera de caña de azúcar (Saccharum officinarum L. con efectos como reductor del colesterol y antioxidante, el cual está compuesto por una mezcla de ácidos grasos libres saturados de elevado peso molecular, cada uno dentro de un intervalo de concentración específica determinada por cromatografía de gases (CG. La caracterización espectroscópica del D-003, sin embargo, no ha sido previamente informada. Objetivo: caracterizar el ingrediente farmacéutico activo nombrado D-003 de acuerdo con sus espectros ultravioleta (UV, infrarrojo (FTIR, resonancia magnética nuclear (RMN y de masas (EM. Métodos: se evaluaron muestras de seis lotes de D-003 (CNIC, Cuba mediante las técnicas de UV, FTIR, RMN-¹H, RMN-13C y CG-EM. Para obtener los espectros de masas de los ácidos del D-003, estos se derivaron como ésteres metílicos y trimetilsilil. La cuantificación de los ácidos grasos libres saturados de elevado peso molecular, analizados como ésteres metílicos, se llevó a cabo por CG con detector de ionización por llama (DILL. Resultados: los espectros UV, IR, RMN-¹H y RMN-13C mostraron que el ingrediente farmacéutico activo D00-3 está constituido por una mezcla de ácidos grasos libres saturados de elevado peso molecular, mientras las técnicas de CG-EM y CG-DILL permitieron asegurar la presencia de 13 ácidos grasos libres saturados de elevado peso molecular en proporciones definidas: C24:0 (0,2-0,6 %, C25:0 (0,4-0,9 %, C26:0 (2,0-3,1 %, C27:0 (2,1-2,7 %, C28:0 (30,0-37,5 %, C29:0 (1,5-1,7 %, C30:0 (17,0-18,6 %, C31:0 (0,9-1,2 %, C32:0 (6,9-8,9 %, C33:0 (0,9-1,3 %, C34:0 (7,2-11,1 %, C35:0 (0,3-0,6 % y C36:0 (2,2-3,8 %. Conclusiones: a partir de las técnicas espectroscópicas de UV, FTIR, RMN, y CG-EM se demuestra que el D00-3 está compuesto por 13 ácidos grasos saturados de elevado peso molecular, entre los cuales el ácido octacosanoico es el

  18. Effect of the litter material on drinking water quality in broiler production

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    RG Garcia

    2010-09-01

    Full Text Available Considering the importance of drinking water and its effect on broiler performance, drinking water quality was studied using six different litter materials. The presence of coliform bacteria and Escherichia coli was investigated. The following litter materials were used in the trial: wood shavings, rice husks, chopped Napier grass (Pennisetum pupureum, 50% sugarcane bagasse (Saccharum L. + 50% wood shavings, 50% sugarcane bagasse (Saccharum L. + 50% rice husks, and plain sugarcane bagasse (Saccharum L.. A number of 1620 Ross® one-day-old chicks were reared in 54 pens measuring 4.5 m² each, equipped with a bell drinker and a tube feeder. Water samples were collected in sterile tubes on days 28 and 42 of the rearing period, and submitted to the laboratory for analyses. Microbiological data were organized by classes expressed in a logarithm scale, where the lowest contamination corresponds to class 1 and the highest contamination to class 4. Results showed that total coliform contamination was higher on day 28 than in the end of the rearing period, and that E. coli presence was detected during both analyzed periods. The litter materials that presented lower degree of water contamination, predominantly class 1, were sugarcane bagasse and 50% of sugarcane bagasse and 50% of rice husks.

  19. Quantification of 16S gene and its relation with the CO2 emission and soil properties in areas under management of sugarcane (Saccharum spp.)

    Science.gov (United States)

    Moitinho, Mara Regina; da Silva Bicalho, Elton; De Bortoli Teixeira, Daniel; La Scala, Newton, Jr.

    2015-04-01

    A diversity of microorganisms has an essential role in the recycling of soil chemical elements, controlling, for example, the dynamics of carbon de)ion and stabilization, and consequently the patterns of soil CO2 emission. In this sense, the objectives of this study were: (i) to estimate and compare the genetic diversity of microorganisms in soils under different sugarcane (Saccharum spp.) managements using molecular techniques based on metagenomic studies, and (ii) investigate the relationship of soil CO2 emission (FCO2) with microbiological results and soil chemical and physical properties in the evaluated managements. This study was conducted in agricultural areas located in southern Brazil, in which the following sugarcane managements were used: green and burned residues management, a sugarcane area under reform, and a native forest (used as a reference of the original soil condition). FCO2, soil temperature, and soil moisture were measured over 10 days, and at the end of the measurements soil samples were taken in order to determine the physical and chemical soil properties. The determination of the diversity of soil microorganisms was carried out by means of molecular techniques based on 16S rRNA gene sequencing. The highest mean value for FCO2 (3.25 μmol m-2s-1) was observed in the sugarcane area under reform, and the lowest values (1.85 and 1.27 μmol m-2s-1) were observed respectively in the green residue management and native forest areas. This same pattern was also observed when the 16S gene was quantified. In this case, the largest number of copies of this gene was found in the sugarcane area under reform (4.3x1010 copies of 16S rRNA gene per gram of dry soil), and its smallest number of copies was found in the green residues management area (1.7x1010 copies of 16S rRNA gene per gram of dry soil). The largest number of copies of the 16S gene associated to the highest values of FCO2, both observed in the sugarcane area under reform, could be related to

  20. Efeitos de hexazinone e diuron, e suas misturas, no controle de capim-de-colchão (Digitaria sanguinalis (L. Scop em cana-de-açúcar (Saccharum spp Effects of hexazinone and diuron and mixtures on crabgrass (Digitaria sanguinalis (L. Scop control on sugarcane (Saccharum spp

    Directory of Open Access Journals (Sweden)

    L. S. P. Cruz

    1983-06-01

    Full Text Available Foi conduzido em 1976 /77, um experimento de campo em área do Centro de Tecnologia da Copersucar, em Piracicaba, SP , com a finalidade de se conhecer o efeito dos herbicidas hexazinone e diuron , assim como o de suas misturas, no controle do capim-de -colchão (Digitaria sanguinalis (L. Scop em avançado estádio de desenvolvimento vegetativo infestando cultura de cana-deaçúcar (Saccharum spp. Os tratamentos constaram da aplicação pós - emergente de hexazinone a 0,30, 0,35, 0,45 e 0, 65 kg/h a; de diuro na 0, 88 , 1, 20 e 2, 50 kg/h a; de hexazinone -i - diuron a 0,30 0,88, 0,35 + 1 , 2 0 e 0 , 4 5 + 1 , 3 6 kg / ha . Foram incluídos mais dois tratamentos com herbicidas ( ter - bacila 0,9 6 kg/ ha e metribuzin a 1,5 0 kg/ ha e um sem herbicida, mantidos empreno limpo com o auxílio de enxada. Esses 13 tratamentos foram distribuídos em blocos ao acaso, com quatro repetições. Foram determinados também os efeitos dos tratamentos sobre a produção de cana -de-açúcar no campo e sobre suas características tecnológicas (Brix, Pol, Pureza, Fibra. Os melhores resultados de controle da gramínea , aos 15 dias da aplicação dos herbicidas , foram obtidos com a mistura de hexazinone a 0, 45 kg/h a com diuron a 1, 36 kg/h a. Hexazinon e a 0,6 4 kg /h a, aplicado isolado, também apresentou bons resultados de controle. Nos tratamentos com hexazinone apareceram sintomas de fitotoxicidade na cana-de-açúcar, os quais desapareceram posteriormente, sem interferir na produção. Os demais tratamentos também não foram prejudiciais à cana de-açúcar.A field experiment was carried out at the Centre of Technology of Copers ucar, Piracicaba , SP, to know the action of hexazinone and diuron and mixtures on crab grass (Digitaria sanguinali s (L . Scop control, at advanced stage of development in sugarcane crop. Treatments were post -emergence aplication of hexazinone at 0,30; 0,35; 0,45 and 0,64 kg/ha; diuron at 0,88; 1,20 ; 1,36 and 2, 50 kg/h a

  1. Radiosensitivity and recovery of tree crowns in a gamma-irradiated northern forest community

    International Nuclear Information System (INIS)

    Buech, R.R.

    1977-01-01

    Crown mortality was observed on 13 tree species in a gamma-irradiated forest community located near Rhinelander, Wis. Observations at the end of the first (1973) and second (1974) postirradiation growing seasons are presented for each species. Crown mortality was most severe during 1973 for Acer rubrum, A. saccharum, Ostrya virginiana, Populus tremuloides, Prunus serotina, Quercus rubra, and Ulmus americana. For some species, however, crown mortality was most severe during 1974. These were Betula alleghaniensis, B. papyrifera, Tilia americana, and possibly Fraxinus americana and F. nigra. These differences in year of primary response are explained on the basis of bud differentiation and mortality. The most resistant species were A. saccharum, O. virginiana, and Populus tremuloides, and the most sensitive were T. americana and U. americana. Others were intermediate in sensitivity. Taken collectively, exposures less than 2 or 3 kr did not seriously affect crowns of most species, whereas exposures exceeding about 40 kr were lethal to the crowns of all tree species. The observed zonation of tree mortality closely approximated predictions published by others except for underestimating the resistance of A. saccharum and overestimating the resistance of T. americana, F. americana, and Prunus serotina. Not surprisingly, the tree stratum of the northern forest community was found to be more radiosensitive than lichen, grassland, or herbaceous communities in other irradiation studies

  2. The relationship between sap-flow rate and sap volume in dormant sugar maples

    Science.gov (United States)

    William J. Gabriel; Russell S. Walters; Donald W. Seegrist

    1972-01-01

    Sap-flow rate is closely correlated with the sap volume produced by dormant sugar maple trees (Acer saccharum Marsh.) and could be used in making phenotypic selections of trees for superior sap production.

  3. Widespread sugar maple decline and regeneration failure in the Adirondacks

    Science.gov (United States)

    Jerry C. Jenkins; Elizabeth Moffett; Daphne Ross

    1999-01-01

    Over large areas of the Adirondacks, hardwood stands whose canopies are dominated by or contain abundant mature sugar maple (Acer saccharum Marsh.) have almost no sugar maple saplings or seedlings in the understory.

  4. In vitropropagation in Temporary Immersion System of sugarcane plants variety `RB 872552' derived from somatic embryos

    Directory of Open Access Journals (Sweden)

    Marina Medeiros de Araújo Silva

    2015-07-01

    Full Text Available In this study, we used a temporary immersion system (TIS to multiply sugarcane (Saccharum spp. plants obtained by somatic embryogenesis (SE. SE was induced from immature leaf segments that were grown in culture medium supplemented with 2,4-D and BAP. Embryo formation occurred in 81% of the inoculated explants and 254 plants were regenerated. Ninety plants were transferred to TIS and cultured in medium supplemented with BAP. After three subcultures, 60 000 plantlets were obtained and transferred to rooting media. After 30 days of acclimatization period plantlets were well developed and exhibited a 96% survival. The results demonstrate the feasibility of the combined use of two important techniques of in vitro culture (SE and shoot multiplication in TIS to sugarcane in vitro propagation. Key words: acclimatization, 6-benzylaminopurine, Saccharum spp.

  5. 2127-IJBCS-Article-Koffi Badou Jérémie

    African Journals Online (AJOL)

    hp

    Determination of critical period for weed control in intensive and non-intensive sugarcane (Saccharum officinarum L., Poaceae) production systems in center Côte d'Ivoire ... and chemical methods for effective and ...... Handbook Series No. 4.

  6. Genetic studies and a search for molecular markers that are linked ...

    African Journals Online (AJOL)

    SERVER

    White sorghum is ground into flour and used ... Eleusine coracana (L.) Gaertn], maize (Zea mays L.), rice. (Oryza sativa L.) and sugar cane (Saccharum species). (Stroud .... ng/ul solutions were prepared and were used in the construction of.

  7. Long-Term Simulated Atmospheric Nitrogen Deposition Alters Leaf and Fine Root Decomposition

    Science.gov (United States)

    Atmospheric nitrogen deposition has been suggested to increase forest carbon sequestration across much of the Northern Hemisphere; slower organic matter decomposition could contribute to this increase. At four sugar maple (Acer saccharum)-dominated northern hardwood forests, we p...

  8. Thwarting plague and pestilence in the Australian sugar industry : Crop protection capacity and resilience built by agricultural extension

    NARCIS (Netherlands)

    Hunt, Warren; Birch, Colin; Vanclay, Frank

    This paper investigates how Australian sugar industry extension services over the last decade have overcome historical pest management challenges in sugarcane (Saccharum spp.). The industry has done this by building increased capacity amongst its extension agents and farmers. This paper considers

  9. Evaluating Brazilian sugarcane expansion effects on soil structure using VESS

    Science.gov (United States)

    Increasing global demand for biofuel has accelerated land-use change (LUC) in Brazil, primarily by replacing degraded pasture with sugarcane (Saccharum officinarum). The intensive echanization associated with this LUC has increased concerns regarding soil structural quality (SSQ). Through decades of...

  10. Development of somaclones in sugarcane genotype BF-162 and ...

    African Journals Online (AJOL)

    pris

    2012-02-21

    Feb 21, 2012 ... Cluster and sub cluster formation further verified the presence of variability in the red rot resistant somaclones with respect to the parent. Key words: Sugarcane, callogenesis, organogenesis, somaclone, polymorphism, cluster. INTRODUCTION. Sugarcane (Saccharum officinarum L) is a major source.

  11. The effects of natural and induced short-term floods on four sugarcane accessions

    Science.gov (United States)

    A study was initiated at the USDA/ARS Subtropical Horticulture Research Station, Miami, FL to evaluate the potential for flood tolerance in several accessions from Saccharum barberi (Jeswiet) and S. sinense (Roxb. Amend. Jeswiet) sugarcane genera. The experimental design included 4 accessions, Kewal...

  12. In situ genomic DNA extraction for PCR analysis of regions of interest in four plant species and one filamentous fungi

    Directory of Open Access Journals (Sweden)

    Luis E. Rojas

    2014-07-01

    Full Text Available The extraction methods of genomic DNA are usually laborious and hazardous to human health and the environment by the use of organic solvents (chloroform and phenol. In this work a protocol for in situ extraction of genomic DNA by alkaline lysis is validated. It was used in order to amplify regions of DNA in four species of plants and fungi by polymerase chain reaction (PCR. From plant material of Saccharum officinarum L., Carica papaya L. and Digitalis purpurea L. it was possible to extend different regions of the genome through PCR. Furthermore, it was possible to amplify a fragment of avr-4 gene DNA purified from lyophilized mycelium of Mycosphaerella fijiensis. Additionally, it was possible to amplify the region ap24 transgene inserted into the genome of banana cv. `Grande naine' (Musa AAA. Key words: alkaline lysis, Carica papaya L., Digitalis purpurea L., Musa, Saccharum officinarum L.

  13. Soil microbial communities buffer physiological responses to drought stress in three hardwood species.

    Science.gov (United States)

    Kannenberg, Steven A; Phillips, Richard P

    2017-03-01

    Trees possess myriad adaptations for coping with drought stress, but the extent to which their drought responses are influenced by interactions with soil microbes is poorly understood. To explore the role of microbes in mediating tree responses to drought stress, we exposed saplings of three species (Acer saccharum, Liriodendron tulipifera, and Quercus alba) to a four week experimental drought in mesocosms. Half of the pots were inoculated with a live soil slurry (i.e., a microbial inoculum derived from soils beneath the canopies of mature A. saccharum, L. tulipifera or Q. alba stands), while the other half of the pots received a sterile soil slurry. Soil microbes ameliorated drought stress in L. tulipifera by minimizing reductions in leaf water potential and by reducing photosynthetic declines. In A. saccharum, soil microbes reduced drought stress by lessening declines in leaf water potential, though these changes did not buffer the trees from declining photosynthetic rates. In Q. alba, soil microbes had no effects on leaf physiological parameters during drought stress. In all species, microbes had no significant effects on dynamic C allocation during drought stress, suggesting that microbial effects on plant physiology were unrelated to source-sink dynamics. Collectively, our results suggest that soil microbes have the potential to alter key parameters that are used to diagnose drought sensitivity (i.e., isohydry or anisohydry). To the extent that our results reflect dynamics occurring in forests, a revised perspective on plant hydraulic strategies that considers root-microbe interactions may lead to improved predictions of forest vulnerability to drought.

  14. Analysis of Three Sugarcane Homo/Homeologous Regions Suggests Independent Polyploidization Events of Saccharum officinarum and Saccharum spontaneum.

    Science.gov (United States)

    Vilela, Mariane de Mendonça; Del Bem, Luiz Eduardo; Van Sluys, Marie-Anne; de Setta, Nathalia; Kitajima, João Paulo; Cruz, Guilherme Marcelo Queiroga; Sforça, Danilo Augusto; de Souza, Anete Pereira; Ferreira, Paulo Cavalcanti Gomes; Grativol, Clícia; Cardoso-Silva, Claudio Benicio; Vicentini, Renato; Vincentz, Michel

    2017-02-01

    Whole genome duplication has played an important role in plant evolution and diversification. Sugarcane is an important crop with a complex hybrid polyploid genome, for which the process of adaptation to polyploidy is still poorly understood. In order to improve our knowledge about sugarcane genome evolution and the homo/homeologous gene expression balance, we sequenced and analyzed 27 BACs (Bacterial Artificial Chromosome) of sugarcane R570 cultivar, containing the putative single-copy genes LFY (seven haplotypes), PHYC (four haplotypes), and TOR (seven haplotypes). Comparative genomic approaches showed that these sugarcane loci presented a high degree of conservation of gene content and collinearity (synteny) with sorghum and rice orthologous regions, but were invaded by transposable elements (TE). All the homo/homeologous haplotypes of LFY, PHYC, and TOR are likely to be functional, because they are all under purifying selection (dN/dS ≪ 1). However, they were found to participate in a nonequivalently manner to the overall expression of the corresponding gene. SNPs, indels, and amino acid substitutions allowed inferring the S. officinarum or S. spontaneum origin of the TOR haplotypes, which further led to the estimation that these two sugarcane ancestral species diverged between 2.5 and 3.5 Ma. In addition, analysis of shared TE insertions in TOR haplotypes suggested that two autopolyploidization may have occurred in the lineage that gave rise to S. officinarum, after its divergence from S. spontaneum. © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  15. Woody tissue analysis using an element ratio technique (DRIS)

    Science.gov (United States)

    Kurt H. Riitters; L.F. Ohmann; D.F. Grigal

    1991-01-01

    The diagnosis and recommendation integrated system (DRIS) was used to describe the variation of 12 elements in woody tree tissue and balsam fir (Abies balsamae (L.) Mill.), sugar maple (Acer saccharum Marsh.), jack pine (Pinus banksiana Lamb.), red pine (Pinus resinosa alt.), and aspen (

  16. Aluminum solubility and mobility in relation to organic carbon in surface soils affected by six tree species of the northeastern United States

    NARCIS (Netherlands)

    Dijkstra, F.A.; Fitzhugh, R.D.

    2003-01-01

    We compared Al solubility and mobility in surface soils among six tree species (sugar maple [Acer saccharum], white ash [Fraxinus americana], red maple [Acer rubrum, L.], American beech [Fagus grandifolia, Ehrh.], red oak [Quercus rubra, L.], and hemlock [Tsuga canadensis, Carr.]) in a mixed

  17. Using normalized difference vegetation index (NDVI) to estimate sugarcane yield and yield components

    Science.gov (United States)

    Sugarcane (Saccharum spp.) yield and yield components are important traits for growers and scientists to evaluate and select cultivars. Collection of these yield data would be labor intensive and time consuming in the early selection stages of sugarcane breeding cultivar development programs with a ...

  18. Utilization of a major brown rust resistance gene in sugarcane breeding

    Science.gov (United States)

    Brown rust, caused by Puccinia melanocephala has had devastating effects on sugarcane (Saccharum spp.) breeding programs and on commercial production. The discovery of Bru1, a major gene conferring resistance to brown rust represented a substantial breakthrough and markers for the detection of Bru1 ...

  19. Problems, control, and opportunity of starch in the large scale processing of sugarcane and sweet sorghum

    Science.gov (United States)

    Both sugarcane (Saccharum officinarum) and sweet sorghum (Sorghum bicolor) crops are members of the grass (Poaceae) family, and consist of stalks rich in soluble sugars. The extracted juice from both of these crops contains insoluble starch, with much greater quantities occurring in sweet sorghum. ...

  20. MICROBIAL COLONIZATION, RESPIRATION, AND BREAKDOWN OF MAPLE LEAVES ALONG A STREAM-MARSH CONTINUUM

    Science.gov (United States)

    Breakdown rates, macroinvertebrate and bacterial colonization, and microbial respiration were measured on decaying maple (Acer saccharum) leaves at three sites along a stream-marsh continuum. Breakdown rates (-k+-SE) were 0.0284+-0.0045 d-1 for leaves in a high-gradient, non-tida...

  1. Antioxidant activity influenced by in vivo and in vitro mutagenesis in ...

    African Journals Online (AJOL)

    The antioxidant potential (1,1-diphenyl-2-picrylhydrazyl (DPPHº)-scavenging activity) of in vitro regenerated and induced mutant sugarcane (Saccharum officinarum L.) was investigated. Efficient callus induction and shoot regeneration were induced in bud explants when incubated on Murashige and Skoog (MS) medium ...

  2. African Journal of Biotechnology - Vol 4, No 4 (2005)

    African Journals Online (AJOL)

    Molecular approaches to detect and study the organisms causing bovine tick borne diseases: babesiosis and anaplasmosis · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT ... Response of sugarcane (Saccharum sp.) varieties to embryogenic callus induction and in ...

  3. Analysis of SSR information in EST resources of sugarcane

    Science.gov (United States)

    Expressed sequence tags ( ESTs) offer the opportunity to exploit single, low -copy, conserved sequence motifs for the development of simple sequence repeats ( SSRs). The total of 262 113 ESTs of sugarcane (Saccharum officinarum) in the database of NCBI were downloaded and analyzed, which resulted in...

  4. Confined Disposal Facility and Maintenance Dredging of the Les Cheneaux Island Federal Navigation Channels, Michigan.

    Science.gov (United States)

    1979-01-01

    Methylation of mercury at levels > 1 mg/kg has been documented (1,2). Methyl mercury is directly available for bioaccumulation in the food chain. Elevated...White Pine, Pinus strobus L. Red Pine, Pinus resinosa Ait. Sugar Maple, Acer saccharum Marsh Yellow Birch, Betula alleghaniensis Britton Basswood

  5. Bulletin of Materials Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science; Volume 31; Issue 1. Pressure induced graft-co-polymerization of acrylonitrile onto Saccharum cilliare fibre and ... Author Affiliations. A S Singha1 Anjali Shama1 Vijay Kumar Thakur1. Material Science Laboratory, National Institute of Technology, Hamirpur 177 005, India ...

  6. Potential of diazotrophic bacteria associated with sugarcane for energycane production

    Science.gov (United States)

    Crosses between sugarcane and wild species of Saccharum and other closely related genera are made to introgress new genes from the wild species into sugarcane. Characteristics of the progeny from these crosses may include increased biomass and the ability to be grown in a broader geographical range ...

  7. Screening for sugarcane brown rust in first clonal stage of the Canal Point sugarcane breeding program

    Science.gov (United States)

    Sugarcane (Saccharum spp.) brown rust (caused by Puccinia melanocephala H. & P. Sydow) was first reported in the United States in 1978 and is still one of great challenges for sugarcane production. A better understanding of sugarcane genotypic variation in response to brown rust will help optimize b...

  8. Glyphosate Control of Orange and Brown Rusts in Glyphosate-Sensitive Sugarcane Cultivars

    Science.gov (United States)

    Technical Abstract: Brown and orange rust diseases cause substantial yield reductions on sugarcane (Saccharum spp.) in Florida and other regions where sugarcane is grown. Brown rust caused by Puccinia melanocephala Syd. & P. Syd has been present in Florida since 1978 and orange rust caused by Pucci...

  9. Effect of orange rust on sugarcane breeding program at canal Point

    Science.gov (United States)

    Orange rust of sugarcane (Saccharum spp. hybrids), caused by Puccinia kuehnii (W. Krüger) E.J. Butler, appeared in the Western Hemisphere ten years ago. Orange rust substantially reduces yields in susceptible sugarcane genotypes. Majority of the commercial cultivars were susceptible at the time of o...

  10. Tree species effects on calcium cycling: The role of calcium uptake in deep soils

    NARCIS (Netherlands)

    Dijkstra, F.A.; Smits, M.M.

    2002-01-01

    Soil acidity and calcium (Ca) availability in the surface soil differ substantially beneath sugar maple (Acer saccharum) and eastern hemlock (Tsuga canadensis) trees in a mixed forest in northwestern Connecticut. We determined the effect of pumping of Ca from deep soil (rooting zone below 20-cm

  11. Foliar nutrient analysis of sugar maple decline: retrospective vector diagnosis

    Science.gov (United States)

    Victor R. Timmer; Yuanxin Teng

    1999-01-01

    Accuracy of traditional foiiar analysis of nutrient disorders in sugar maple (Acer saccharum Marsh) is limited by lack of validation and confounding by nutrient interactions. Vector nutrient diagnosis is relatively free of these problems. The technique is demonstrated retrospectively on four case studies. Diagnostic interpretations consistently...

  12. Acute O3 damage on first year coppice sprouts of aspen and maple sprouts in an open-air experiment

    Science.gov (United States)

    Joseph N.T. Darbah; Wendy S. Jones; Andrew J. Burton; John Nagy; Mark E. Kubiske

    2011-01-01

    We studied the effect of high ozone (O3) concentration (110-490 nmol mol-1) on regenerating aspen (Populus tremuloides) and maple (Acer saccharum) trees at an open-air O3 pollution experiment near Rhinelander WI USA. This study is the first of its kind to examine...

  13. Exploration of indigenous agrowastes for cellulase production by ...

    African Journals Online (AJOL)

    Regional agrowastes such as Vigna mungo, Saccharum spontaneum and Brassica campestris were collected and biohydrolysis of these substrates for cellulase production were carried out by Aspergillus niger. Proximate composition of each agrowastes was analyzed based on dry weight, to have an insight view of their ...

  14. Sugarcane field residue and bagasse allelopathic impact on vegetable seed germination

    Science.gov (United States)

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the allelopathic impact of sugarcane (Saccharum officinarum) var. ‘HoCP 96-540’ field residue and sugarcane baga...

  15. Eleven-year response of foliar chemistry to chronic nitrogen and sulfur additions at the Bear Brook Watershed in Maine

    Science.gov (United States)

    Jose Alexander Elvir; Lindsey Rustad; G. Bruce Wiersma; Ivan Fernandez; Alan S. White; Gregory J. White

    2005-01-01

    The foliar chemistry of sugar maple (Acer saccharum Marsh.), American beech (Fagus grandifolia Ehrh.), and red spruce (Picea rubens Sarg.) was studied from 1993 to 2003 at the Bear Brook Watershed in Maine (BBWM). The BBWM is a paired-watershed forest ecosystem study, with one watershed treated bimonthly since...

  16. Genotyping Sugarcane for the Brown Rust Resistance Locus Bru1 Using Unlabeled Probe Melting

    Science.gov (United States)

    Brown rust, caused by the fungus Puccinia melanocephala, is a major disease of sugarcane (Saccharum spp.) in Florida, Louisiana, and other sugarcane growing regions. The Bru1 locus has been used as a durable and effective source of resistance, and markers are available to select for the trait. The...

  17. Registration of ‘CP 09-1822’ Sugarcane

    Science.gov (United States)

    ‘CP 09-1822’ (Reg. No. __; PI 686942 sugarcane (a complex hybrid of Saccharum spp.) was released in June 2016 for commercial cultivation on sand (mineral) soils in Florida. This cultivar was developed through a collaborative sugarcane cultivar development program of the USDA-ARS, the University of F...

  18. Fabrication and characterization of S. cilliare fibre reinforced

    Indian Academy of Sciences (India)

    In the recent times, there has been an ever-increasing interest in green composite materials for its applications in the field of industries, aerospace, sports, household etc and in many other fields. In this paper, fabrication of Saccharum cilliare fibre reinforced green polymer composites using resorcinol formaldehyde (RF) as ...

  19. simple sequence repeats (EST-SSR)

    African Journals Online (AJOL)

    Yomi

    2012-01-19

    Jan 19, 2012 ... 212 primer pairs selected, based on repeat patterns of n≥8 for di-, tri-, tetra- and penta-nucleotide repeat ... Cluster analysis revealed a high genetic similarity among the sugarcane (Saccharum spp.) breeding lines which could reduce the genetic gain in ..... The multiple allele characteristic of SSR com-.

  20. The complex relationship between climate and sugar maple health: Climate change implications in Vermont for a key northern hardwood species

    Science.gov (United States)

    Evan M. Oswald; Jennifer Pontius; Shelly A. Rayback; Paul G. Schaberg; Sandra H. Wilmot; Lesley-Ann Dupigny-Giroux

    2018-01-01

    This study compared 141 ecologically relevant climate metrics to field assessments of sugar maple (Acer saccharum Marsh.) canopy condition across Vermont, USA from 1988 to 2012. After removing the influence of disturbance events during this time period to isolate the impact of climate, we identified five climate metrics that were significantly...

  1. The effect of in-vitro environmental conditions on some sugarcane ...

    African Journals Online (AJOL)

    ajl yemi

    in-vitro morphogenetic responses of leaf sheath explants of sugarcane varieties, CoS 96258 and CoS. 99259. High frequency callus initiation was recorded in leaf sheath explants incubated in dark for 10 ... tool for rapid and large scale production of disease free ... propagation of Saccharum varieties using RAPD analysis.

  2. cas du cordon littoral Port-Bouët-Grand-Bassam

    African Journals Online (AJOL)

    Formations végétales. Espèces. Familles. Cocoteraie. Fourré littoral. Jachère. Saccharum officinarum L. Poaceae x. Salacia baumannii Loes. Celastraceae x. Sansevieria liberica Gérôme & Labroy. Agavaceae x. Sauvagesia erecta L. Ochnaceae x. Scoparia dulcis L. Scrophulariaceae x x. Sesamum radiatum Schumacher.

  3. Differential impacts of calcium and aluminum treatments on sugar maple and American beech growth dynamics

    Science.gov (United States)

    Joshua M. Halman; Paul G. Schaberg; Gary J. Hawley; Christopher F. Hansen; Timothy J. Fahey

    2015-01-01

    Acid deposition induced losses of calcium (Ca) from northeastern forests have had negative effects on forest health for decades, including the mobilization of potentially phytotoxic aluminum (Al) from soils. To evaluate the impact of changes in Ca and Al availability on sugar maple (Acer saccharum Marsh.) and American beech (Fagus...

  4. Sugar maple height-diameter and age-diameter relationships in an uneven-aged northern hardwood stand

    Science.gov (United States)

    Laura S. Kenefic; R.D. Nyland

    1999-01-01

    Sugar maple (Acer saccharum Marsh.) height-diameter and age-diameter relationships are explored in a balanced uneven-aged northern hardwood stand in central New York. Results show that although both height and age vary considerably with diameter, these relationships can be described by statistically valid equations. The age-diameter relationship...

  5. Registration of ‘CP 09-1430’ Sugarcane

    Science.gov (United States)

    ‘CP 09-1430’ (Reg. No. ; PI 686940 sugarcane (a complex hybrid of Saccharum spp.) was developed and released (6 Jun. 2016) through cooperative research conducted by the USDA-ARS Sugarcane Field Station , Canal Point, the University of Florida, and the Florida Sugar Cane League, Inc. for use on ...

  6. First Report of Orange Rust of Sugarcane caused by Puccinia kuehnii in Ecuador

    Science.gov (United States)

    Orange rust, Puccinia kuehnii (W. Krüger) E.J. Butler, is an important disease of sugarcane (complex hybrid of Saccharum L. species) that causes yield losses, and impacts breeding programs. Initially confined to the Asia-Oceania region (5), P. kuehnii was reported in Florida in June 2007 (2) and lat...

  7. Calcium and aluminum impacts on sugar maple physiology in a northern hardwood forest

    Science.gov (United States)

    Joshua M. Halman; Paul G. Schaberg; Gary J. Hawley; Linda H. Pardo; Timothy J. Fahey

    2013-01-01

    Forests of northeastern North America have been exposed to anthropogenic acidic inputs for decades, resulting in altered cation relations and disruptions to associated physiological processes in multiple tree species, including sugar maple (Acer saccharum Marsh.). In the current study, the impacts of calcium (Ca) and aluminum (Al) additions on mature...

  8. Discovering the desirable alleles contributing to the lignocellulosic biomass traits in saccharum germplasm collections for energy cane improvement

    Energy Technology Data Exchange (ETDEWEB)

    Todd, James [USDA ARS Sugarcane Field Station, Canal Point, FL (United States); Comstock, Jack C. [USDA ARS Sugarcane Field Station, Canal Point, FL (United States)

    2015-11-25

    of the cores and the World Collection are similar to each other genotypically and phenotypically, but the core that was selected using only genotypic data was significantly different phenotypically. This indicates that there is not enough association between the genotypic and phenotypic diversity as to select using only genotypic diversity and get the full phenotypic diversity. Core Collection: Creation and Phenotyping Methods: To evaluate this germplasm for breeding purposes, a representative diversity panel selected from the WCSRG of approximately 300 accessions was planted at Canal Point, FL in three replications. These accessions were measured for stalk height and stalk number multiple times throughout the growing season and Brix and fresh biomass during harvest in 2013 and, stalk height, stalk number, stalk diameter, internode length, Brix and fresh and dry biomass was determined in the ratoon crop harvest in 2014. Results: In correlations of multiple measurements, there were higher correlations for early measurements of stalk number and stalk height with harvest traits like Brix and fresh weight. Hybrids had higher fresh mass and Brix while Saccharum spontaneum had higher stalk number and dry mass. The heritability of hybrid mass traits was lower in the ratoon crop. According to the principal component analysis, the diversity panel was divided into two groups. One group had accessions with high stalk number and high dry biomass like S. spontaneum and the other groups contained accessions with higher Brix and fresh biomass like S. officinarum. Mass traits correlated with each other as expected but hybrids had lower correlations between fresh and dry mass. Stalk number and the mass traits correlated with each other except in S. spontaneum and hybrids in the first ratoon. There were 110 accessions not significantly different in Brix from the commercial sugarcane checks including 10 S. spontaneum accessions. There were 27 dry and 6 fresh mass accessions

  9. Soil water and xylem chemistry in declining sugar maple stands in Pennsylvania

    Science.gov (United States)

    David R. DeWalle; Bryan R. Swistock; William E. Sharpe

    1999-01-01

    Evidence is accumulating that decline of sugar maple, Acer saccharum Marsh., in northern Pennsylvania may be related to overall site fertility as reflected in the chemistry of soil water and bolewood xylem. In this paper we discuss factors related to varying site fertility, including effects of soil liming, past glacialion, topographic position and...

  10. Cold-season patterns of reserve and soluble carbohydrates in sugar maple and ice-damaged trees of two age classes following drought

    Science.gov (United States)

    B. L. Wong; K. L. Baggett; A. H. Rye

    2009-01-01

    This study examines the effects of summer drought on the composition and profiles of cold-season reserve and soluble carbohydrates in sugar maple (Acer saccharum Marsh.) trees (50-100 years old or ~200 years old) in which the crowns were nondamaged or damaged by the 1998 ice storm. The overall cold season reserve...

  11. Restoring pine barrens for avian conservation

    Science.gov (United States)

    Greg Corace

    2001-01-01

    At first glance, many visitors to Michigan's Upper Peninsula (U.P.) see a fairly uniform forested region. Although northern hardwood forests comprised of sugar maple (Acer saccharum), American basswood (Tilia americana), yellow birch (Betula alleghaniensis) and eastern hemlock (Tsuga canadensis) predominate, the U.P. is a fact a mosaic of forest cover types...

  12. Preliminary results of sugar maple carbohydrate and growth response under vacuum and gravity sap extraction

    Science.gov (United States)

    Mark L. Isselhardt; Timothy D. Perkins; Abby K. van den Berg; Paul G. Schaberg

    2016-01-01

    Recent technological advancements have increased the amount of sugar-enriched sap that can be extracted from sugar maple (Acer saccharum). This pilot study quantified overall sugar removal and the impacts of vacuum (60 cm Hg) and gravity sap extraction on residual nonstructural carbohydrate (NSC) concentrations and on stem and twig growth. Vacuum...

  13. Effects of uneven-aged and diameter-limit management on West Virginia tree and wood quality

    Science.gov (United States)

    Michael C. Wiemann; Thomas M. Schuler; John E. Baumgras

    2004-01-01

    Uneven-aged and diameter-limit management were compared with an unmanaged control on the Fernow Experimental Forest near Parsons, West Virginia, to determine how treatment affects the quality of red oak (Quercus rubra L.), sugar maple (Acer saccharum Marsh.), and yellow-poplar (Liriodendron tulipifera L.). Periodic harvests slightly increased stem lean, which often...

  14. Sugar maple seedling anatomy and element localization at forest sites with differing nutrient levels

    Science.gov (United States)

    Carolyn J. McQuattie; Robert P. Long; Thomas J. Hall

    1999-01-01

    Sugar maple (Acer saccharum Marsh.) seedlings often have poor survival on acidic unglaciated portions of the Allegheny Plateau. Greater survival is found after lime treatment of unglaciated sites or on glaciated areas of the Plateau. The difference in survival rate may depend in part on the acidity or chemical composition of the soil.

  15. Registration of 'CP 09-2392' Sugarcane

    Science.gov (United States)

    CP 09-2392’ (Reg. No.____; PI _____) sugarcane, a complex hybrid of Saccharum spp, was developed through cooperative research conducted by the USDA-ARS, the University of Florida, and the Florida Sugar Cane League, Inc., and was released to growers in June 2016. ‘CP 09-2392’ was selected from a cro...

  16. Linum usitatissimum

    African Journals Online (AJOL)

    SAM

    2014-03-19

    Mar 19, 2014 ... . Dalri AB, Cruz RL (2002). Efeito da freqüência de irrigação subsuperf- icial por gotejamento no desenvolvimento da cana-de-açúcar. (Saccharum spp.). Irriga, Botucatu 7(1):29-34. De Paula MB, Carvalho VD, Nogueira FD (1992). Efeitos da vinhaça na.

  17. Influence of experimental snow removal on root and canopy physiology of sugar maple trees in a northern hardwood forest

    Science.gov (United States)

    Daniel P. Comerford; Paul G. Schaberg; Pamela H. Templer; Anne M. Socci; John L. Campbell; Kimberly F. Wallin

    2013-01-01

    Due to projected increases in winter air temperatures in the northeastern USA over the next 100 years, the snowpack is expected to decrease in depth and duration, thereby increasing soil exposure to freezing air temperatures. To evaluate the potential physiological responses of sugar maple (Acer saccharum Marsh.) to a reduced snowpack, we measured...

  18. Bulletin of Materials Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science. A S Singha. Articles written in Bulletin of Materials Science. Volume 31 Issue 1 February 2008 pp 7-13 Polymers. Pressure induced graft-co-polymerization of acrylonitrile onto Saccharum cilliare fibre and evaluation of some properties of grafted fibre · A S Singha Anjali Shama ...

  19. Evaluation of sugarcane ( Saccharum officinarum L.) somaclonal ...

    African Journals Online (AJOL)

    A total of four plants which regenerated from the tolerant calli were selected but the best in vigor were grown in in vitro and hydroponic systems under salinity stress (with the previous levels) as compared to source variety. With increasing supply of NaCl in both systems, root growth was more adversely affected than shoot ...

  20. Rapid micropropagation of three elite Sugarcane (Saccharum ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-07-04

    Jul 4, 2008 ... ... Droevendaalsesteeg-1 6708 PB, Wageningen, The Netherlands. ... Best response of multiplication for variety CP-77-400 was observed ... Rooting response was observed on half strength liquid MS medium ..... day demand.

  1. Technical Efficiency of Sugar Cane ( Saccharum officinarum ...

    African Journals Online (AJOL)

    Journal of Agriculture, Forestry and the Social Sciences ... frontier model shows that experience of the farmer and household size had significant effect on the ... such as fertilizers and sugarcane cuttings available to farmers at a subsidized rate.

  2. Coil irrigation in sugar cane (Saccharum officinarum

    Directory of Open Access Journals (Sweden)

    Jesús Sánchez Gutiérrez

    2016-01-01

    Full Text Available This study was made at the Basic Seed Bank of the Local Sugar Cane Research Station for the Mideastern Cuba, based in Camaguey, on brown carbonate soil, between 2013 and 12014. Coil irrigation was applied to meet the water requirements for the crop, according to the edafoclimatic conditions and the different phenological phases it has. The Savo method was used to determine useful rain water. Adjustment and complementation of the irrigation program was based on indicators that characterize the exploitation scheme. The machine´s working parameters were determined to meet the water needs, and increase crop´s overall yields. The evaluations and results achieved have contributed to new proposals for management and operation of coil irrigation, and they are important to increase its efficiency.

  3. Nutritional and antimicrobial evaluation of Saccharum officinarum ...

    African Journals Online (AJOL)

    Tuoyo Aghomotsegin

    2016-08-17

    Aug 17, 2016 ... and polyphenols as powerful antioxidants (Palmer, 2005;. Martinez and Martinez .... sample were determined by the methods of the Association of. Official Analytical ... Some of the phytochemical compounds were also quantified using ..... P 175. Vila FC, Colombo R, de Lira TO, Yariwake JH (2008). HPLC.

  4. Ground biomass assessment of shrub species in tehsil takht-e-nasrati, pakistan

    International Nuclear Information System (INIS)

    Khan, M.; Hussain, F.; Musharaf, S.; Musharaf, S.

    2014-01-01

    The shrub biomass of different species of Tehsil Takht-e-Nasrati was different at different altitude. In the present study it was found that the average shrub biomass was decreasing with increasing altitude. Result confirms that the biomass of Saccharum bengalense was high 5020.38 Kg.hec/sup -1/ in phase 1 and phase 2 (4331.58 Kg.hec/sup -1/). The highest ground biomass 1125.1 Kg.hec/sup -1/ of Zizyphus nummularia was found in Phase 3. Furthermore in Phase 4 the biomass of Capparis deciduas was high 437.79 Kg.hec/sup -1/. Along with shrubs average biomass of Saccharum bengalense was high 2665.12 Kg.hec/sup -1/ and low 13.47 Kg.hec/sup -1/ of Cassia angustifolia. With seasons the biomass of Saccharum bengalense (13800 Kg.hec/sup -1/) was greater during winter at Phase 1 and Periploc aaphylla (12.35 Kg.hec-1) biomass was lowers during spring at Phase 4. In comparison in season the biomass was high in winter due to the dormant stage of shrubs in phase 1 while it was low in summer in phase 4. In winter the grazing process was stop due to agriculture point of view while in hilly area the grazing was high and the low percentage of rain fall consequently the biomass was high in plain area as contrast to hilly area. The biomass of shrubs is high in winter while it was low in summer as the grazing and palatability rate was high in summer as well as low in winter. The assessment of shrub biomass in research area is a requirement for successful management at the same time as it gives a complete documentation for the area in complexity and work out unpredictable resources to help imagine shrubs potency and behavior. (author)

  5. Potential causes of the pear thrips outbreak in sugar maple

    Science.gov (United States)

    Jack C. Schultz

    1991-01-01

    No one knows what caused the 1988 outbreak of pear thrips, Taeniothrips inconsequens (Uzel), in sugar maple, Acer saccharum Marsh., in the northeastern United States. As an entomologist and ecologist who knows even less about this insect than most of the authors of this volume, I cannot presume to understand the causes of this...

  6. Building improved models of sugar maple mortality

    Science.gov (United States)

    Charles H. Perry; Patrick L. Zimmerman

    2012-01-01

    The decline of sugar maple (Acer saccharum Marsh.) in the northern United States is causing concern, and several studies have identified soil properties that are linked to the observation of dead/dying trees. Unfortunately, the sample of trees supporting these studies is purposive in nature; soil properties are assessed only on those plots where dead...

  7. Evaluation of Sugar Maple Dieback in the Upper Great Lakes Region and Development of a Forest Health Youth Education Program

    Science.gov (United States)

    Bal, Tara L.

    2013-01-01

    Sugar Maple, "Acer saccharum" Marsh., is one of the most valuable trees in the northern hardwood forests. Severe dieback was recently reported by area foresters in the western Upper Great Lakes Region. Sugar Maple has had a history of dieback over the last 100 years throughout its range and different variables have been identified as…

  8. Development of somaclones in sugarcane genotype BF-162 and ...

    African Journals Online (AJOL)

    pris

    2012-02-21

    Feb 21, 2012 ... Baksha R, Alam R, Karim MZ, Paul SK, Hossain MA, Miah MAS,. Rahman ABMM (2002). In vitro Shoot Tip Culture of Sugarcane. (Saccharum officinarum) Variety Isd 28. Int. Quarterly J. Biotech. 1: 67-72. Brisibe E, Miyake AH, Taniguchi T, Maeda E (1994). Regulation of somatic embryogenesis in long-term ...

  9. Effects of ice storm damage on hardwood survival and growth in Ohio

    Science.gov (United States)

    Richard M. Turcotte; Thomas R. Elliott; Mary Ann Fajvan; Yong-Lak Park; Daniel A. Snider; Patrick C. Tobin

    2012-01-01

    In 2003, an ice storm occurred across four Mid-Atlantic states. This study investigated the effects of the ice-storm damage on growth and mortality of five tree species (Acer rubrum, Acer saccharum, Quercus alba, Quercus prinus, and Quercus rubra) from three forest stands in the Wayne National Forest in Ohio. We remeasured the same...

  10. Assessing the invasive potential of biofuel species proposed for Florida and the United States using the Australian Weed Risk Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Gordon, D.R. [The Nature Conservancy, PO Box 118526, University of Florida, Gainesville, FL 32611 (United States); Department of Biology, PO Box 118526, University of Florida, Gainesville, FL 32611 (United States); Tancig, K.J. [PO Box 116455, University of Florida, Gainesville, FL 32611 (United States); Onderdonk, D.A.; Gantz, C.A. [Department of Biology, PO Box 118526, University of Florida, Gainesville, FL 32611 (United States)

    2011-01-15

    Twelve taxa under exploration as bioenergy crops in Florida and the U.S. were evaluated for potential invasiveness using the Australian Weed Risk Assessment system (WRA) modified for separate assessment at the state and national scales. When tested across a range of geographies, this system correctly identifies invaders 90%, and non-invaders 70% of the time, on average. Predictions for Florida were the same as for the U.S. Arundo donax, Eucalyptus camaldulensis, Eucalyptus grandis, Jatropha curcas, Leucaena leucocephala, Pennisetum purpureum, and Ricinus communis were found to have a high probability of becoming invasive, while Miscanthus x giganteus, Saccharum arundinaceum, Saccharum officinarum, and the sweet variety of Sorghum bicolor have a low probability of becoming invasive. Eucalyptus amplifolia requires further evaluation before a prediction is possible. These results are consistent with reports on other tests of these taxa. Given the economic and ecological impacts of invasive species, including the carbon expended for mechanical and chemical control efforts, cultivation of taxa likely to become invasive should be avoided. (author)

  11. Effects of defoliation and drought on root food reserves in sugar maple seedlings

    Science.gov (United States)

    Johnson Parker; Johnson Parker

    1970-01-01

    The artificial defoliation of sugar maple (Acer. saccharum Marsh.) can cause a marked decline in root food reserves, especially starch, and an increase in the levels of the reducing sugars, fructose and glucose. Defoliation can also bring on the dieback-decline syndrome in sugar maples (Parker and Houston 1968). Two experiments designed to examine this question were...

  12. Population dynamics of sugar maple through the southern portion of its range: implications for range migration

    Science.gov (United States)

    Justin L. Hart; Christopher M. Oswalt; Craig M. Turberville

    2014-01-01

    The range of sugar maple (Acer saccharum Marsh.) is expected to shift northward in accord with changing climate. However, a pattern of increased sugar maple abundance has been reported from sites throughout the eastern US. The goal of our study was to examine the stability of the sugar maple southern range boundary by analyzing its demography through...

  13. A sugar maple planting study in Vermont

    Science.gov (United States)

    Harry W. Yawney; Clayton M., Jr. Carl; Clayton M. Carl

    1970-01-01

    Past attempts to establish sugar maple (Acer saccharum Marsh.) by planting have generally met with little success. The failures have been blamed mainly on competition by other vegetation and on damage done by animals. Finding an effective way to establish sugar maple seedlings is a key part in the research being carried on in Vermont by the USDA Forest Service to...

  14. Use of damage surveys and field inventories to evaluate oak and sugar maple health in the northern United States

    Science.gov (United States)

    Randall S Morin; Christopher W. Woodall; Jim Steinman; Charles H. Perry

    2009-01-01

    Oak species (Quercus spp.) and sugar maple (Acer saccharum) are substantial components of the forest ecosystems in the 24-state region spanning the northern U.S. During recent decades, both damage surveys and forest inventories have documented declines of sugar maple and oak health. In order to more fully assess the status of oak and sugar maple health, we examined...

  15. Black bark as an indicator of bird peck defect in sugar maple

    Science.gov (United States)

    John H. Ohman; K.J., Jr. Kessler

    1964-01-01

    Portions of the lower bole of occasional sugar maples (Acer saccharum Marsh.) in Lake States northern hardwood stands are sooty black in sharp contrast to the normal gray. The blackened areas may encircle the entire stem or be confined to a narrow band; they have been observed as high as 25 feet but generally extend about 15 feet above the ground....

  16. Stimulatory effects of aluminum on growth of sugar maple seedlings

    Science.gov (United States)

    George A. Schier; Carolyn J. McQuattie

    2002-01-01

    To determine the effect of aluminum (Al) on sugar maple (Acer saccharum Marsh.), seedlings were grown in sand irrigated with nutrient solution (pH 3.8) containing 0, 2.5, 5, 10, 20, or 40 mg L-1 Al. Seedling growth was enhanced at 2.5 and 5mgL-1 Al. Although higher levels of Al reduced calcium (Ca) and...

  17. Maturation of sugar maple seed

    Science.gov (United States)

    Clayton M., Jr. Carl; Albert G., Jr. Snow; Albert G. Snow

    1971-01-01

    The seeds of a sugar maple tree (Acer saccharum Marsh.) do not mature at the same time every year. And different trees mature their seeds at different times. So time of year is not a reliable measure of when seeds are ripe. Better criteria are needed. In recent studies we have found that moisture content and color are the best criteria for judging when sugar maple...

  18. Saccharum spp. en Brasil. Una revisión

    Directory of Open Access Journals (Sweden)

    Samuel Córdova Sanchez

    2014-01-01

    Full Text Available El objetivo del presente trabajo es analizar las bondades y efectos negativos de la caña de caña de azúcar en Brasil. En 2012, se cosecharon 9’075,388 hectáreas, cultivadas en nueve suelos, con humedad crítica, energía solar de 6,350 MJ m -2 año, temperatura entre 30-45ºC, la precipitación entre 1,500 a 2,500 mm, en el crecimiento y 100 mm en la maduración de la planta. Las grandes extensiones del monocultivo causan inestabilidad ecológica. Se ha detectado la infestación del cultivo con más de 23 especies de arvenses. La tendencia es aplicar el manejo integrado de plagas. La cosecha se realiza en el periodo de abril-noviembre. Para la zafra de 2014-2015, será prohibida la quema de la caña en áreas donde se practica la cosecha mecanizada; y para 2017, las áreas no mecanizadas. Del total del área cultivada, el 88.80% de la super - ficie puede ser cosechada con máquinas, lo que permite obtener residuos de cosecha, entre 10- 20 t ha -1 ; esta cantidad de residuos es efectiva para el manejo de arvenses. Las pérdidas en el rendimiento por arvenses varían de 10 a 100%; las peores arvenses que infestan los residuos de cosecha son especies de Cyperaceae, Poaceae y Convolvulaceae . Los disemínulos pueden ser transportados por vientos huracanados hasta 1,000 km, inundaciones 100 km, erosión 10 km, derrumbe, 1 km. Para el control de arven - ses, en Brasil, se han registrado 47 ingredientes activos de herbicidas; de éstos, se aplicaron para el control de plagas agrícolas durante 2012, 823.20 mil t de productos químicos.

  19. Biohydrolysis of Saccharum spontaneum for cellulase production by ...

    African Journals Online (AJOL)

    admin

    2012-03-13

    Mar 13, 2012 ... fermentation (SSF) for economic production of cellulase. ... Lignocellulosic biomass has been regarded as a ... concentration in the pretreated substrate. It can be fairly ... wheat straw, 54% and sorghum straw, 61%) for ethanol.

  20. Seasonal changes of cytokinins in upper and lower leaves of a sugar maple crown

    International Nuclear Information System (INIS)

    Held, M.A.; Quesnelle, P.E.; Emery, R.J.N.

    2005-01-01

    Although it is well accepted that cytokinins (CKs) regulate processes such as leaf senescence and stomatal conductance, data on CKs in the canopy of mature trees are lacking in the literature. Here we report the first in situ sampling for determination of CKs in mature sugar maple (Acer saccharum) canopy layers. The upper canopy showed a distinct seasonal pattern in total CK content, whereas the lower canopy remained relatively unchanged

  1. Flood Tolerance in Plants: A State-of-the-Art Review

    Science.gov (United States)

    1979-08-01

    Q imbriraria Black walnut Juaus nigra Bitternut hickory Carya cordiformi. Red bud Cerc:i n e adensis Box el.der Accr n,ýgundo Slippery elm Jlmus rubra...Liriodendron tl__ipifera), sugar maple (Acer saccharum), white oak (Quercus alba), honey-locust (Gleditsia triacanthos), and Amterican elm (Ulmus americana...only American elm developed adventitious roots (YClcnouky 1964). 37. Accumulation of C02 . Next to lowered 0 concentration,:; the accumulation of toxic

  2. Abundance of food plant species and food habits of Rhinoceros unicorns Linn. in Pobitora Wildlife Sanctuary, Assam, India

    Directory of Open Access Journals (Sweden)

    P. Konwar

    2009-09-01

    Full Text Available Food habits and abundance of food plant species of Rhinoceros unicornis in Pobitora Wildlife Sanctuary were studied from January 1999 through December 2001. Totally 32 numbers of Rhino food plants were identified, of which 15 were grasses, four shrubs, five aquatic hydrophytes and eight tree species (21 terrestrial and 11 aquatic. During the dry season, the Rhino feeds on almost 90% food items from Hemarthria compressa, Arundo donax, Phragmites karka, Cerex rubro-brumee etc. The other short grasses such as Cynodon dactylon, Andropogon ssp., Cenchrus ciliaris, Chrysopogon aciculatus and tender and young shoots and twigs of Schelristechya fuesche, Saccharum spontaneum, Lagerstroemia flosreginae etc. are consumed in limited portions. The rhino consumes 11 cultivated crops and vegetables, viz., Ricinus communis, Oryza sativa, Solanum melongena, Lycopersicon esculentum, Solanum tuberosum, Brassica nigra, Luffa cylindrica, Luffa acutangula, Cucurbita moschata, Cucumis sativus and Ipomoea batatas etc. Highest density of food plant species observed in the study area were Cynodon dactylon (167.5/m2, Hemarthria compressa (73.75/m2, Vetiveria zizanioides (56/m2, Saccharum ravannae (51.5/m2, Pharagmites karka (50.75/m2, Leersia hexandra (46.75/m2, Brachiarea pseudointerrupta (40/m2 and Eichhornia crassipes (35/m2.

  3. Chemical control of weeds during the acclimatization of in vitro sugarcane plants cv. 'CP52-43'

    Directory of Open Access Journals (Sweden)

    Inoel García Ruiz

    2016-07-01

    Full Text Available Control of weeds in the acclimatization phase of in vitro sugarcane (Saccharum spp. plants is done manually which raises the cost of production. In order to determine the effectiveness of the chemical control of weeds with Dual Gold CE 96 (Mesotrione, in vitro plants of sugar cane cv. 'CP52-43' were acclimatized in worm humus and cachaça compost. Doses of 0.5, 1.0 and 1.5 l ha-1 p.c. Dual Gold CE 96 herbicide, were applied before and immediately to in vitro plants transplantation and the results were compared to manual weeding. After 50 days of culture, 15 plants were extracted per treatment and the number of leaves and shoots were quantified, the length was measured and the fresh mass of the aerial part and of the roots were determined. The results showed that Dual Gold CE 96 at doses of 0.5 l ha-1 applied before or after transplant, controls the weeds Portulaca oleracea (L., Amaranthus sp. (Mart, Sida acuta (Burm. F. and Eleusine indica (L., without affecting the growth of sugarcane cv. ‘CP52-43’ plants, compared to manual weeding.   Keywords: herbicides, Saccharum, substrate, survival

  4. Seasonal variations in food plant preferences of reintroduced Rhinos Rhinoceros unicornis (Mammalia: Perrissodactyla: Rhinocerotidae in Manas National Park, Assam, India

    Directory of Open Access Journals (Sweden)

    Deba Kumar Dutta

    2016-11-01

    Full Text Available The food preferences of translocated Rhinos in Manas National Park were studied to find out variations in seasonal and annual preferences.  A total of 139 plants species belonging to 39 families were observed to be consumed as food.  On an average, grasses (n=33 contributed 24% of Rhino food, aquatic plants (n=23 16.5%, shrubs (n=11 7.5%, herbs (n = 31 22.3% trees (n=26 18.7%, creepers (n=3 2.1% and agricultural crops (n=12 8.6%.  Among the grasses, throughout the year Arundo donax, Cynodon dactylon, Imperata cylindrica, Saccharum elephantinus and Saccharum spontaneum were the maximum preferred species.  Rhinos were observed to browse shrubs and tree twigs during the winter season and browsing was found to be very limited during the monsoon due to the abundance of young grass.  Various anthropogenic pressures such as unregulated grassland burning, cattle grazing, invasions of Bombax ceiba and shrubs like Chromolaena odorata, Leea asiatica and herbs like Ageratum conyzoides have degraded some of the important grasslands.  So, a proper grassland management protocol including the burning of grasslands during the dry season, keeping grazing animals away and control of weeds is suggested in the areas extensively used by the Rhinos. 

  5. Histopatology of the compatible and incompatible interaction between Puccinia melanocephala and sugar cane var. B4362

    OpenAIRE

    María I. Oloriz; Luis Rojas; Víctor Gil; Orelvis Portal; Elio Jiménez

    2008-01-01

    Induction of mutations is an alternative for genetic improvement of sugarcane (Saccharum spp. hybrid). It has been used to obtain mutants with resistance to rust (Puccinia melanocephala). Characteristics of pathogen infection process were identified through histopathological studies in a mutant (IBP 8518) of the B4362 variety, with resistance to this disease. Fragments of leaves were inoculated with P. melanocephala . Then, these were stained with lactophenol tripan blue to observe, in the op...

  6. Evaluation of sugarcane introgression lines for resistance to brown rust disease caused by Puccinia melanocephala

    OpenAIRE

    Wang, Xiao-Yan; Wen-Feng, Li; Ying-Kun, Huang; Xin, Lu; Zhi-Ming, Luo; Jiong, Yin; Hong-Li, Shan; Rong-Yue, Zhang

    2013-01-01

    Sugarcane brown rust disease caused by Puccinia melanocephala is one of the important fungal diseases affecting sugarcane yield around the world. Cultivar resistance is the most appropriate control method for this disease. In this study, 62 introgression lines chosen from the crossing Saccharum officinarum L. cv. Ludashi x Erianthus rockii Yunnan 95-19 were evaluated for brown rust resistance using artificial inoculation. More than 30% of the introgression lines were identified as resistant. ...

  7. Jackson Mills and Mine Falls Dams, Nashua, New Hampshire. Reconnaissance Report, Hydroelectric Feasibility. Volume 1, Jackson Mills Dam.

    Science.gov (United States)

    1979-12-01

    Catalpa Solidago sp. Goldenrod Aster novae - angliae New England Aster Acer saccharum Sugar Maple Ulmus rubra Slippery elm Solanum hi rum Common...red pine, and hemlock are the common softwood species, and the common hardwood species include red maple, silver maple, white oak, willow, slippery ... elm and birch. In 1972, between 70 and 7S percent of the total area of the watershed consisted of forests and primarily wooded land. (Reference 3

  8. Cultivation of Agaricus blazei ss. Heinemann using different soils as source of casing materials Cultivo de Agaricus blazei ss. Heinemann usando diferentes solos como fonte de material para a camada de cobertura

    Directory of Open Access Journals (Sweden)

    Félix Gonçalves de Siqueira

    2009-12-01

    Full Text Available Commercial productivity of the Agaricus blazei mushroom is closely related to both the quality of the compost and the choice of soil to be used as a casing material. This study aims to evaluate Agaricus blazei's productivity using two compost formulations and three soils. The two compost formulations were (i crushed sugarcane bagasse (Saccharum officinarum (L. and Coastcross hay (Cynidon dactylon (L. Pers., and (ii crushed sugarcane bagasse (Saccharum officinarum (L. and corn husk (Zea mays L.; they were amended with wheat bran, lime, gypsum, superphosphate and urea. The casing materials were extracted from three soils classed as Rhodic Hapludox, Xanthic Hapludox, and Humic Haplaquox. The Rhodic Hapludox soil material was mixed with fragments of Eucalyptus charcoal in the proportion of 4:1. The compost was prepared during six weeks and thereafter heat treated during 48 h at the end of the composting period. The sugarcane bagasse:coast-hay compost was superior to the sugarcane bagasse: corn husk compost. The Rhodic Hapludox plus charcoal casing material showed to be a better casing material than either the Xanthic Hapludox and Humic Haplaquox soil materials. The choice of the soils where the casing material is taken is an important factor to the success of the Agaricus blazei mushroom cultivation.A produtividade do cogumelo Agaricus blazei depende da qualidade do composto e da terra de cobertura. Avaliou-se a produtividade deste cogumelo utilizando-se duas formulações de composto e materiais retirados de três tipos de solo usados como camada de cobertura. As duas formulações de composto consistiram em (i bagaço de cana-de-açúcar (Saccharum officinarum (L. e capim Coastcross (Cynidon dactylon (L. Pers., e (ii bagaço de cana-de-açúcar (Saccharum officinarum (L. e palha de milho (Zea mays L.. As duas formulações foram suplementadas com farelo de trigo, calcário, gesso, superfosfato simples e uréia. Como camadas de cobertura foram

  9. Establishment of in vitro callus in sugarcane (Saccharum officinarum ...

    African Journals Online (AJOL)

    Admin

    2016-07-20

    Jul 20, 2016 ... Tissue culture technique, an alternative method for solving production ... NAA). All the auxins were applied in 0.0, 0.5, 1.0, 2.0 and 3.0 mg L-1. MS basal .... Data were analysed by analysis of variance (ANOVA) using computer ...

  10. Plant growth inhibitors isolated from sugarcane (Saccharum officinarum) straw.

    Science.gov (United States)

    Sampietro, Diego Alejandro; Vattuone, Marta Amelia; Isla, María Ines

    2006-07-01

    Several compounds related with plant defense and pharmacological activities have been isolated from sugarcane. Straw phytotoxins and their possible mechanisms of growth inhibition are largely unknown. A bioassay-guided fractionation of the phytotoxic constituents leachated from a sugarcane straw led to the isolation of trans-ferulic (trans-FA), cis-ferulic (cis-FA), vanillic (VA) and syringic (SA) acids. The straw leachates and their identified constituents significantly inhibited root growth of lettuce and four weeds. VA was more phytotoxic to root elongation than FA and SA. The identified phenolic compounds significantly increased leakage of root cell constituents, inhibited dehydrogenase activity and reduced chlorophyll content in lettuce. VA and FA inhibited mitotic index while SA increased cell division. Additive (VA-FA and FA-SA) and synergistic (VA-SA) interactions on root growth were observed at the response level of EC(25). Although the isolated compounds differed in their relative phytotoxic activities, the observed physiological responses suggest that they have a common mode of action. HPLC analysis indicated that sugarcane straw can potentially release 1.43 (ratio 2:1, trans:cis), 1.14 and 0.14mmolkg(-1) (straw dry weight) of FA, VA and SA, respectively. As phenolic acids are often found spatially concentrated in the top soil layers under plant straws, further studies are needed to establish the impact of these compounds in natural settings.

  11. Estimation of herbicide bioconcentration in sugarcane (Saccharum officinarum L.

    Directory of Open Access Journals (Sweden)

    Antonio Luiz Cerdeira

    2015-04-01

    Full Text Available Sugarcane is an important crop for sugar and biofuel production in Brazil. Growers depend greatly on herbicides to produce it. This experiment used herbicide physical-chemical and sugarcane plant physiological properties to simulate herbicide uptake and estimate the bioconcentration factor (BCF. The (BCF was calculated for the steady state chemical equilibrium between the plant herbicide concentration and soil solution. Plant-water partition coefficient (sugarcane bagasse-water partition coefficient, herbicide dilution rate, metabolism and dissipation in the soil-plant system, as well as total plant biomass factors were used. In addition, we added Tebuthiuron at rate of 5.0kg a.i. ha-1 to physically test the model. In conclusion, the model showed the following ranking of herbicide uptake: sulfentrazone > picloram >tebuthiuron > hexazinone > metribuzin > simazine > ametryn > diuron > clomazone > acetochlor. Furthermore, the highest BCF herbicides showed higher Groundwater Ubiquity Score (GUS index indicating high leaching potential. We did not find tebuthiuron in plants after three months of herbicide application

  12. Phytochemical profile of sugarcane and its potential health aspects

    OpenAIRE

    Singh, Amandeep; Lal, Uma Ranjan; Mukhtar, Hayat Muhammad; Singh, Prabh Simran; Shah, Gagan; Dhawan, Ravi Kumar

    2015-01-01

    Sugarcane (Saccharum officinarum Linn.) is an important perennial grass of Poaceae family, indigenous to tropical South Asia and Southeast Asia. It is cultivated worldwide due to the economical and medicinal value of its high yielding products. Sugarcane juice is well known as a raw material for the production of refined sugar and its wax is considered as a potential substitute for the expensive carnauba wax, which is of cosmetic and pharmaceutical interest. Refined sugar is the primary produ...

  13. Lignin-Furfural Based Adhesives

    OpenAIRE

    Dongre, Prajakta; Driscoll, Mark; Amidon, Thomas; Bujanovic, Biljana

    2015-01-01

    Lignin recovered from the hot-water extract of sugar maple ( Acer saccharum ) is used in this study to synthesize adhesive blends to replace phenol-formaldehyde (PF) resin. Untreated lignin is characterized by lignin content and nuclear magnetic resonance (NMR) analysis. The molecular weight distribution of the lignin and the blends are characterized by size exclusion chromatography (SEC). The effect of pH (0.3, 0.65 and 1), ex situ furfural, and curing conditions on the tensile properties of...

  14. Sterilization of African Violet in the in Vitro Culture Using Synthesized Silver Nanoparticles by Two Plant Extracts

    Directory of Open Access Journals (Sweden)

    M. Solgi

    2015-12-01

    Full Text Available One of the major advantages of in vitro culture of African violet (Saintpaulha ionantha is production of new cultivars and propagation of their chimera which might not be propagated by the other methods. In this study, we tested the effects of silver nanoparticles on the sterilization rate (antifungal and antibacterial activity, regeneration and shoot formation of African violet "Pink Amiss" explants. These nanoparticles were synthesized from pomegranate peels and Damask rose petals extracts. We used a completely randomized design test with factorial arrangement to investigate various volumetric ratios of plant extracts to silver nitrate (1:20, 1:10, 1:5 and 1:1 on the culture contaminations. Using silver nanoparticles synthesized by the plant extracts, especially Damask rose petals extract resulted in no fungal and bacterial contamination in the African violet explants after 1 and 3 weeks as compared to the control, and silver nitrate (1mM. All tested concentrations of the silver nanoparticles significantly (P &le 0.05 controlled both bacterial and fungal contaminations. The 1:20 ratio of plant extracts to silver nitrate showed the best control. In addition, the highest regeneration (%52 and shoot regeneration (%38 was observed in this treatment. In conclusion, we suggest using silver nanoparticles synthesized by plant extracts for sterilization of in Vitro Culture for African Violet rather than using other chemicals such as silver nitrate.

  15. Evidence of prehistoric Lapita diet at Watom Island, Papua New Guinea, using stable isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Leach, B F [Archaeozoology Laboratory, Museum of New Zealand, Wellington (New Zealand); Quinn, C J [178 Queen Street North, Dunedin (New Zealand); Lyon, G L [Institute of Geological and Nuclear Sciences Ltd., Lower Hutt (New Zealand); Haystead, A [Auckland Institute of Technology, Auckland (New Zealand); Myers, D B [Wellcome Medical Research Institute, Otago Medical School, Dunedin (New Zealand)

    1998-07-01

    Samples of human bone from six individuals from the Lapita burial ground at Reber-Rakival on Watom Island in New Britain were analysed for {delta}{sup 13}C, {delta}{sup 15}N, and {delta}{sup 34}/S. The mean values obtained were -18.1, 11.6 and 9.9 respectively. From existing knowledge of isotope values, calorific content and protein yields for the main Pacific food types, computer simulation was used to randomly generate a large number of possible food compositions, in order to find the type of diet which could have produced the isotope pattern at Watom. The simulation produced solutions which are within acceptable limits of the Watom isotope signature. The mean food composition per day was then estimated. This analysis shows that approximately 64% of the diet at Watom came from land-based foods and 36% from the sea. Plant foods contributed 53% per weight. It is notable that C4 plants were present in the diet. There are two possible sources of this - sugar cane, Saccharum officinarum, and/or a herbivore which browsed on the C4 grasslands of Papua New Guinea, such as Saccharum spontaneum (pit-pit) and Imperata cylindrica (kunai). Fish and land herbivores are the main sources of protein in the Watom diet, while plant foods contributed by far the most food energy. (author). 20 refs., 2 figs., 3 tabs.

  16. Evidence of prehistoric Lapita diet at Watom Island, Papua New Guinea, using stable isotopes

    International Nuclear Information System (INIS)

    Leach, B.F.; Quinn, C.J.; Lyon, G.L.; Haystead, A.; Myers, D.B.

    1998-01-01

    Samples of human bone from six individuals from the Lapita burial ground at Reber-Rakival on Watom Island in New Britain were analysed for δ 13 C, δ 15 N, and δ 34 /S. The mean values obtained were -18.1, 11.6 and 9.9 respectively. From existing knowledge of isotope values, calorific content and protein yields for the main Pacific food types, computer simulation was used to randomly generate a large number of possible food compositions, in order to find the type of diet which could have produced the isotope pattern at Watom. The simulation produced solutions which are within acceptable limits of the Watom isotope signature. The mean food composition per day was then estimated. This analysis shows that approximately 64% of the diet at Watom came from land-based foods and 36% from the sea. Plant foods contributed 53% per weight. It is notable that C4 plants were present in the diet. There are two possible sources of this - sugar cane, Saccharum officinarum, and/or a herbivore which browsed on the C4 grasslands of Papua New Guinea, such as Saccharum spontaneum (pit-pit) and Imperata cylindrica (kunai). Fish and land herbivores are the main sources of protein in the Watom diet, while plant foods contributed by far the most food energy. (author). 20 refs., 2 figs., 3 tabs

  17. Use of AFLP marker system on sugarcane somaclones to study their resistance to rust

    OpenAIRE

    María Ileana Oloriz; Luis Rojas; Víctor Gil; Milady Mendoza; Ariel Arencibia; Elva Rosa Carmona; Elio Jiménez

    2002-01-01

    AFLPs (amplified fragment length polymorphism) was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca: MseI/atg and EcoRI/aca: MseI/agg). Six polymorphic bands were obtained, two of these only appeared in the resistant genotypes, which are probably DNA sequences, related to rust resistance locus. These fragments have been cloned to study thei...

  18. Reeds as potential sources of alcohol

    Energy Technology Data Exchange (ETDEWEB)

    Lodh, A B; Rao, P R

    1964-01-01

    Five species of reeds, Erianthus ravennae, Saccharum munja S. procerum, Phragmites communis, and Neyraudia reynaudiana yielded 25.02, 19.5, 24.11, 26.1, and 21.6% reducing sugars, repectively., when digested with 1% H/sub 2/SO/sub 4/ under 15lb/square inch for 3 hours. Fermentable sugars from hydrolyzates of the above reeds were 70.0, 34.5, 65.0, 28.0, and 67.5% respectively. This source can become important only in case of an acute demand for fermentation alcohol.

  19. In situ genomic DNA extraction for PCR analysis of regions of interest in four plant species and one filamentous fungi

    OpenAIRE

    Luis E. Rojas; Maritza Reyes; Naivy Pérez-Alonso; María I. Olóriz; Laisyn Posada-Pérez; Bárbara Ocaña; Orelvis Portal; Borys Chong-Pérez; Jorge L. Pérez Pérez

    2014-01-01

    The extraction methods of genomic DNA are usually laborious and hazardous to human health and the environment by the use of organic solvents (chloroform and phenol). In this work a protocol for in situ extraction of genomic DNA by alkaline lysis is validated. It was used in order to amplify regions of DNA in four species of plants and fungi by polymerase chain reaction (PCR). From plant material of Saccharum officinarum L., Carica papaya L. and Digitalis purpurea L. it was possible to extend ...

  20. Patterns of radial and shoot growth of five tree species in a gamma-irradiated northern Wisconsin forest

    International Nuclear Information System (INIS)

    Zavitkovski, J.; Buech, R.R.; Rudolph, T.D.; Bauer, E.O.

    1977-01-01

    Patterns of radial and shoot growth of Abies balsamea, Acer rubrum, A. saccharum, Betula papyrifera, and Populus tremuloides were observed before (1970) and during (1972) gamma-irradiation of forest communities in the Enterprise Radiation Forest. Measurements were made during the growing season along the radiation gradient, and year days (YD) of 10, 25, 50, 75, and 90 percent of total growth were obtained by interpolation. The experimental area was divided into an ''affected'' and a ''no-effect'' zone. The boundary of the affected zone coincided with radiation exposures that effectively reduced the 1972 radial growth of a given species in comparison to the preirradiation growth. In 1970 and in the no-effect zone in 1972, shoot growth of the four broadleaved species started and terminated earlier than the radial growth. In A. balsamea the radial growth started earlier and terminated later than the shoot growth. In all five species, duration of radial growth was significantly longer than that of shoot growth. Radial growth of A. rubrum, A. saccharum, and B. papyrifera started significantly earlier in 1972 than in 1970, but no difference between years was found in the early-starting A. balsamea and P. tremuloides. In contrast, shoot growth of all five species started earlier in 1970 than in 1972. It is suggested that temperature regimes during the early developmental stages were probably responsible for the difference. In the affected zone in 1972, the radiation depressed radial growth and changed its normal pattern in all five species

  1. Transport biofuel yields from food and lignocellulosic C{sub 4} crops

    Energy Technology Data Exchange (ETDEWEB)

    Reijnders, L. [IBED University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam (Netherlands)

    2010-01-15

    In the near future, the lignocellulosic C{sub 4} crops Miscanthus and switchgrass (Panicum virgatum) are unlikely to outcompete sugarcane (Saccharum officinarum) in net energetic yearly yield of transport biofuel ha{sup -1}. This holds both for the thermochemical conversion into liquid hydrocarbons and the enzymatic conversion into ethanol. Currently, Miscanthus and switchgrass would also not seem able to outcompete corn (Zea mays) in net energetic yearly yield of liquid transport biofuel ha{sup -1}, but further development of these lignocellulosic crops may gradually lead to a different outcome. (author)

  2. AISLAMIENTO Y CONTROL MICROBIOLÓGICO DE Leuconostoc mesenteroides, EN UN INGENIO PARA OPTIMIZAR EL RENDIMIENTO DE AZÚCAR Y ETANOL ISOLAMENTO E Leuconostoc Mesenteroides CONTROLE MICROBIOLÓGICO EM UM TALENTO PARA OTIMIZAR O DESEMPENHO DE AÇÚCAR E ETANOL ISOLATION AND MICROBIOLOGICAL CONTROL OF Leuconostoc mesenteroides, IN TO SUGAR REFINERY TO OPTIMIZE THE PERFORMANCE OF SUGAR AND ETHANOL

    OpenAIRE

    RAÚL A CUERVO MULET; JOHANNY ÁNGEL LEDESMA; JORGE ANTONIO DURÁN VANEGAS; FRANCISCO E ARGOTE VEGA

    2010-01-01

    La caña de azúcar (Saccharum officinarum L) es exprimida para obtener su jugo que después de ser purificado y neutralizado, deja cristalizar el azúcar. Considerando la importancia que representa la caña de azúcar para la industria azucarera, se hace necesario incrementar el contenido de sacarosa libre de dextrana (destrucción de sacarosa causada por la acción de microorganismos acompañantes de la caña, siendo uno de los más importantes Leuconostoc mesenteroides). En este trabajo de investigac...

  3. AISLAMIENTO Y CONTROL MICROBIOLÓGICO DE Leuconostoc mesenteroides, EN UN INGENIO PARA OPTIMIZAR EL RENDIMIENTO DE AZÚCAR Y ETANOL

    OpenAIRE

    CUERVO MULET, RAÚL A; ÁNGEL LEDESMA, JOHANNY; DURÁN VANEGAS, JORGE ANTONIO; ARGOTE VEGA, FRANCISCO E

    2010-01-01

    La caña de azúcar (Saccharum officinarum L) es exprimida para obtener su jugo que después de ser purificado y neutralizado, deja cristalizar el azúcar. Considerando la importancia que representa la caña de azúcar para la industria azucarera, se hace necesario incrementar el contenido de sacarosa libre de dextrana (destrucción de sacarosa causada por la acción de microorganismos acompañantes de la caña, siendo uno de los más importantes Leuconostoc mesenteroides). En este trabajo de investigac...

  4. Methyl gallate is a natural constituent of maple (Genus Acer) leaves.

    Science.gov (United States)

    Abou-Zaid, Mamdouh M; Lombardo, Domenic A; Nozzolillo, Constance

    2009-01-01

    Methyl gallate was found in ethanolic extracts of red maple (Acer rubrum L.), silver maple (A. saccharinum L.) and sugar maple (A. saccharum Marsh) leaves, but more was present in methanolic extracts. The increased amount of methyl gallate in methanolic extracts was accompanied by a disappearance of m-digallate. It is concluded that only some of the methyl gallate detected in methanolic extracts is an artefact as a result of methanolysis of m-digallate. Its presence in ethanolic extracts is evidence that it is also a natural constituent of maple leaves.

  5. Informative genomic microsatellite markers for efficient genotyping applications in sugarcane.

    Science.gov (United States)

    Parida, Swarup K; Kalia, Sanjay K; Kaul, Sunita; Dalal, Vivek; Hemaprabha, G; Selvi, Athiappan; Pandit, Awadhesh; Singh, Archana; Gaikwad, Kishor; Sharma, Tilak R; Srivastava, Prem Shankar; Singh, Nagendra K; Mohapatra, Trilochan

    2009-01-01

    Genomic microsatellite markers are capable of revealing high degree of polymorphism. Sugarcane (Saccharum sp.), having a complex polyploid genome requires more number of such informative markers for various applications in genetics and breeding. With the objective of generating a large set of microsatellite markers designated as Sugarcane Enriched Genomic MicroSatellite (SEGMS), 6,318 clones from genomic libraries of two hybrid sugarcane cultivars enriched with 18 different microsatellite repeat-motifs were sequenced to generate 4.16 Mb high-quality sequences. Microsatellites were identified in 1,261 of the 5,742 non-redundant clones that accounted for 22% enrichment of the libraries. Retro-transposon association was observed for 23.1% of the identified microsatellites. The utility of the microsatellite containing genomic sequences were demonstrated by higher primer designing potential (90%) and PCR amplification efficiency (87.4%). A total of 1,315 markers including 567 class I microsatellite markers were designed and placed in the public domain for unrestricted use. The level of polymorphism detected by these markers among sugarcane species, genera, and varieties was 88.6%, while cross-transferability rate was 93.2% within Saccharum complex and 25% to cereals. Cloning and sequencing of size variant amplicons revealed that the variation in the number of repeat-units was the main source of SEGMS fragment length polymorphism. High level of polymorphism and wide range of genetic diversity (0.16-0.82 with an average of 0.44) assayed with the SEGMS markers suggested their usefulness in various genotyping applications in sugarcane.

  6. Revisiting Meiosis in Sugarcane: Chromosomal Irregularities and the Prevalence of Bivalent Configurations

    Directory of Open Access Journals (Sweden)

    Maria Lucia C. Vieira

    2018-06-01

    Full Text Available Traditional sugarcane cultivars (Saccharum officinarum proved highly susceptible to diseases, and this led breeders to progress to interspecific crosses resulting in disease resistance. A backcrossing program to S. officinarum was then required to boost sucrose content. Clonal selection across generations and incorporation of other germplasm into cultivated backgrounds established the (narrow genetic base of modern cultivars (Saccharum spp., which have a man-made genome. The genome complexity has inspired several molecular studies that have elucidated aspects of sugarcane genome constitution, architecture, and cytogenetics. However, there is a critical shortage of information on chromosome behavior throughout meiosis in modern cultivars. In this study, we examined the microsporogenesis of a contemporary variety, providing a detailed analysis of the meiotic process and chromosome association at diakinesis, using FISH with centromeric probes. Chromosomal abnormalities were documented by examining high quality preparations of pollen mother cells (700 in total. Approximately 70% of the cells showed abnormalities, such as metaphase chromosomes not lined up at the plate, lagging chromosomes and chromosomal bridges, and tetrad cells with micronuclei. Some dyads with asynchronous behavior were also observed. Due to the hybrid composition of the sugarcane genome, we suggest that bivalent incomplete pairing may occur in the first prophase leading to univalency. The presence of rod bivalents showing the lagging tendency is consistent with a reduction in chiasma frequency. Finally, the presence of chromatin bridges indicates the indirect occurrence of chromosomal inversions, although chromosome fragments were not clearly recognized. Possible reasons for such meiotic abnormalities and the large prevalence of bivalent formation are discussed.

  7. Probability of foliar injury for Acer sp. based on foliar fluoride concentrations.

    Science.gov (United States)

    McDonough, Andrew M; Dixon, Murray J; Terry, Debbie T; Todd, Aaron K; Luciani, Michael A; Williamson, Michele L; Roszak, Danuta S; Farias, Kim A

    2016-12-01

    Fluoride is considered one of the most phytotoxic elements to plants, and indicative fluoride injury has been associated over a wide range of foliar fluoride concentrations. The aim of this study was to determine the probability of indicative foliar fluoride injury based on Acer sp. foliar fluoride concentrations using a logistic regression model. Foliage from Acer nedundo, Acer saccharinum, Acer saccharum and Acer platanoides was collected along a distance gradient from three separate brick manufacturing facilities in southern Ontario as part of a long-term monitoring programme between 1995 and 2014. Hydrogen fluoride is the major emission source associated with the manufacturing facilities resulting with highly elevated foliar fluoride close to the facilities and decreasing with distance. Consistent with other studies, indicative fluoride injury was observed over a wide range of foliar concentrations (9.9-480.0 μg F -  g -1 ). The logistic regression model was statistically significant for the Acer sp. group, A. negundo and A. saccharinum; consequently, A. negundo being the most sensitive species among the group. In addition, A. saccharum and A. platanoides were not statistically significant within the model. We are unaware of published foliar fluoride values for Acer sp. within Canada, and this research provides policy maker and scientist with probabilities of indicative foliar injury for common urban Acer sp. trees that can help guide decisions about emissions controls. Further research should focus on mechanisms driving indicative fluoride injury over wide ranging foliar fluoride concentrations and help determine foliar fluoride thresholds for damage.

  8. Genotypic variation in transpiration efficiency due to differences in photosynthetic capacity among sugarcane-related clones.

    Science.gov (United States)

    Li, Chunjia; Jackson, Phillip; Lu, Xin; Xu, Chaohua; Cai, Qing; Basnayake, Jayapathi; Lakshmanan, Prakash; Ghannoum, Oula; Fan, Yuanhong

    2017-04-01

    Sugarcane, derived from the hybridization of Saccharum officinarum×Saccharum spontaneum, is a vegetative crop in which the final yield is highly driven by culm biomass production. Cane yield under irrigated or rain-fed conditions could be improved by developing genotypes with leaves that have high intrinsic transpiration efficiency, TEi (CO2 assimilation/stomatal conductance), provided this is not offset by negative impacts from reduced conductance and growth rates. This study was conducted to partition genotypic variation in TEi among a sample of diverse clones from the Chinese collection of sugarcane-related germplasm into that due to variation in stomatal conductance versus that due to variation in photosynthetic capacity. A secondary goal was to define protocols for optimized larger-scale screening of germplasm collections. Genotypic variation in TEi was attributed to significant variation in both stomatal and photosynthetic components. A number of genotypes were found to possess high TEi as a result of high photosynthetic capacity. This trait combination is expected to be of significant breeding value. It was determined that a small number of observations (16) is sufficient for efficiently screening TEi in larger populations of sugarcane genotypes The research methodology and results reported are encouraging in supporting a larger-scale screening and introgression of high transpiration efficiency in sugarcane breeding. However, further research is required to quantify narrow sense heritability as well as the leaf-to-field translational potential of genotypic variation in transpiration efficiency-related traits observed in this study. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  9. Caracterización de hojas de mazorca de maíz y de bagazo de caña para la elaboración de una pulpa celulósica mixta

    OpenAIRE

    Prado-Martínez, Maribel; Anzaldo-Hernández, José; Becerra-Aguilar, Bruno; Palacios-Juárez, Hilda; Vargas-Radillo, José de Jesús; Rentería-Urquiza, Maite

    2012-01-01

    En este artículo se presentan los resultados obtenidos en la caracterización química y morfológica de las hojas de mazorca de maíz (Zea mays) y del bagazo de caña (Saccharum officinarum L.). Se analizaron porcentajes de α-celulosa, holocelulosa, lignina y cenizas, según las Normas Tappi para cada componente y el método de Jayme-Wise. Se determinaron las propiedades biométricas de las fibras de cada planta (longitud y diámetro, grosor de la pared y diámetro del lumen). Además, se estimaron los...

  10. Desempenho efetivo e influência no sistema de limpeza da colheita mecanizada de cana-de-açúcar utilizando diferentes velocidades de deslocamento e rotações do extrator primário

    OpenAIRE

    Martins, Murilo Battistuzzi [UNESP

    2016-01-01

    A cana-de-açúcar (Saccharum spp.) se destaca como uma das principais culturas do agronegócio brasileiro. A colheita mecanizada dessa cultura se faz necessária, atualmente, em função de leis ambientais. Com a mecanização da colheita de cana-de-açúcar, sem a necessária adaptação do canavial surgiram fatores adversos à utilização desta técnica, pelo elevado custo e baixa qualidade da colheita; porém, essas questões estão sendo estudadas e resolvidas melhorando, gradativamente, sua eficiência ope...

  11. Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum

    Directory of Open Access Journals (Sweden)

    Shakra Jamil

    2017-01-01

    Full Text Available Sugarcane contributes 60–70% of annual sugar production in the world. Somaclonal variation has potential to enhance genetic variation present within a species. Present study was done to optimize an in vitro propagation protocol for sugarcane. The experiments included four varieties, 9 callus induction media, 27 regeneration media, and 9 root induction media under two-factor factorial CRD. Data were recorded on callus induction, embryogenic callus formation, shoot elongation (cm, root induction, and plant regeneration. Statistically significant differences existed between genotypes and treatments for callus induction (%, embryogenic callus formation (%, shoot elongation (cm, root induction, and plant regeneration (%. All parameters showed dependency on genotypes, culture media, and their interaction. Highest callus induction (95% embryogenic callus formation (95% was observed in callus induction media 5. Highest plantlet regeneration (98.9% capacity was observed in regeneration media 11 whereas maximum shoot elongation (12.13 cm and root induction (8.32 were observed in rooting media 4. G1 showed best response for all traits and vice versa for G4. Hence it was concluded that G1, callus induction media 5, regeneration media 11, and rooting media 4 are the best conditions for in vitro propagation of sugarcane.

  12. In vitro mutagenesis and selection for salinity tolerance and other agronomic characters in sugarcane

    International Nuclear Information System (INIS)

    Vaidya, E.R.; Patade, V.Y.; Patil, V.D.; Suprasanna, P.; Bapat, V.A.

    2006-01-01

    Full text: Sugarcane (Saccharum spp. Hybrids) is a highly polyploid plant (2n=36-170) grown in different parts of the world from the tropics to subtropics, and accounts for around 60% of the world's sugar. It is also one of the important cash crops in many developing/developed countries, with a high trade value. In this study, in vitro mutagenesis was employed in the selection of salt tolerance in popular sugarcane (Saccharum officinarum L.) cultivars CoC- 671, Co 86032 and Co 94012. Embryogenic callus cultures were subjected to gamma irradiation at different doses (10, 20, 30, 40 and 50). Radio sensitivity estimation indicated that 20 Gy irradiated cultures exhibited almost 50 per cent survival response. Relative growth rate and cell mass accumulation were recorded after irradiation and 30 days of post- irradiation culture. The plants regenerated from the calli irradiated at 20, 30 and 40 Gy were hardened and field planted to study their performance. The embryogenic callus cultures were also exposed to inhibitory levels of NaCl (42.8, 85.6, 128.3, 171.1, 213.9, 256.7 mM). The biochemical responses to the salt stress were studied in terms of proline, glycine betaine accumulation, membrane stability and cell viability. The variegated plants were also observed following gamma irradiation and were analyzed by different molecular markers (RAPD and Ac homologous transposons). The salt selected plants were further field evaluated and the desirable clones are now being evaluated in the second generation for their genetic stability. The proper evaluation of these induced variants with desirable traits may prove useful for cultivation under stress conditions

  13. An automatic on-line 2,2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography method for high-throughput screening of antioxidants from natural products.

    Science.gov (United States)

    Lu, Yanzhen; Wu, Nan; Fang, Yingtong; Shaheen, Nusrat; Wei, Yun

    2017-10-27

    Many natural products are rich in antioxidants which play an important role in preventing or postponing a variety of diseases, such as cardiovascular and inflammatory disease, diabetes as well as breast cancer. In this paper, an automatic on-line 2,2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography (DPPH-HPLC) method was established for antioxidants screening with nine standards including organic acids (4-hydroxyphenylacetic acid, p-coumaric acid, ferulic acid, and benzoic acid), alkaloids (coptisine and berberine), and flavonoids (quercitrin, astragalin, and quercetin). The optimal concentration of DPPH was determined, and six potential antioxidants including 4-hydroxyphenylacetic acid, p-coumaric acid, ferulic acid, quercitrin, astragalin, and quercetin, and three non-antioxidants including benzoic acid, coptisine, and berberine, were successfully screened out and validated by conventional DPPH radical scavenging activity assay. The established method has been applied to the crude samples of Saccharum officinarum rinds, Coptis chinensis powders, and Malus pumila leaves, consecutively. Two potential antioxidant compounds from Saccharum officinarum rinds and five potential antioxidant compounds from Malus pumila eaves were rapidly screened out. Then these seven potential antioxidants were purified and identified as p-coumaric acid, ferulic acid, phloridzin, isoquercitrin, quercetin-3-xyloside, quercetin-3-arabinoside, and quercetin-3-rhamnoside using countercurrent chromatography combined with mass spectrometry and their antioxidant activities were further evaluated by conventional DPPH radical scavenging assay. The activity result was in accordance with that of the established method. This established method is cheap and automatic, and could be used as an efficient tool for high-throughput antioxidant screening from various complex natural products. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Projeções de risco de produção de cana-de-açúcar no Estado de São Paulo baseadas em simulações multimodelos e cenários climáticos futuros

    OpenAIRE

    Helena Maria Soares Pinto

    2015-01-01

    A cana-de-açúcar (Saccharum officinarum) é uma cultura de relevância socioeconômica para o Brasil por proporcionar divisas na balança comercial do país e figurar como pilar estratégico para a matriz energética brasileira. O Estado de São Paulo é o maior produtor nacional de cana e de seus derivados. Apesar da intensa discussão em torno das mudanças climáticas globais na última década, sabe-se que as projeções acerca do clima futuro e seu impacto na agricultura contêm uma série de incertezas o...

  15. Uso de la repetibilidad clonal en la selección de ambientes en ensayos regionales de variedades de caña de azúcar en los estados Lara y Yaracuy

    OpenAIRE

    De Sousa-Vieira, Orlando; Rea, Ramón; Briceño, Rosaura

    2003-01-01

    El término "repetibilidad clonal" se refiere a la correlación existente entre diferentes plantas de un mismo clon. Esta técnica es muy útil para medir la efectividad de la selección de clones de caña de azúcar (Saccharum sp., híbrido) y para estimar la magnitud de la interacción genotipo x ambiente. Este trabajo se realizó con el objeto de ubicar localidades y épocas de zafra similares para rendimiento de caña (TCH) y Pol % caña a fin de disminuir racionalmente su número y desplazar recursos ...

  16. Clarificação de caldo de cana-de-açúcar por peróxido de hidrogênio: efeito da presença de dextrana

    OpenAIRE

    SARTORI,Juliana Aparecida de Souza; MAGRI,Nathália Torres Corrêa; AGUIAR,Claudio Lima de

    2015-01-01

    Resumo A qualidade do açúcar cristal está diretamente associada à qualidade da cana-de-açúcar (Saccharum sp.) entregue nas usinas e à eficiência do processo industrial. A dextrana é considerada um parâmetro de qualidade de matéria-prima, uma vez que sua presença indica que a cana-de-açúcar sofreu deterioração entre as etapas de corte e seu processamento. Durante o processamento do caldo, a dextrana pode interferir na eficiência do processo. Na etapa de clarificação, a sulfitação tem como prin...

  17. Time domain-nuclear magnetic resonance study of chars from southern hardwoods

    International Nuclear Information System (INIS)

    Elder, Thomas; Labbe, Nicole; Harper, David; Rials, Timothy

    2006-01-01

    Chars from the thermal degradation of silver maple (Acer saccharinum), red maple (Acer rubrum), sugar maple (Acer saccharum), and white oak (Quercus spp.), performed at temperatures from 250 to 350 o C, were examined using time domain-nuclear magnetic resonance spectroscopy. Prior to analysis, the chars were equilibrated under conditions insuring the presence of bound water only and both bound water and free water. Transverse relaxation times were found to be related to the moisture content of the chars, which varied with temperature. At elevated temperatures the number of signals assigned to free water decreased, indicative of an increase in pore size within the chars

  18. Impacto del tráfico de equipos durante la cosecha de caña de azúcar (Saccharum officinarum Impact of traffic equipment during sugarcane (Saccharum officinarum harvest

    Directory of Open Access Journals (Sweden)

    Luís A. Rodríguez

    2012-10-01

    Full Text Available Para determinar el impacto del tráfico sobre el suelo, el cultivo y el consumo energético durante la cosecha de caña de azúcar en el valle del río Cauca (Colombia, se establecieron experimentos de cuatro repeticiones con diferentes sistemas de cosecha. En cada sitio se cosecharon mecánicamente parcelas con vagones de auto volteo, HD8000, HD12000 y HD20000, se evaluaron por la intensidad de tráfico (IT, el pisoteo directo sobre la cepa, la resistencia a la penetración y el consumo energético. Vagones grandes y pesados causaron mayor IT y mayor efecto por compactación y pisoteo. La IT varió entre 241 y 317 Mg km ha-1. El pisoteo en la cabecera varió de 8 a 18 m por surco y sobre la cepa los vagones pisaron entre 5 y 24% de su ancho. Hubo diferencias no significativas en productividad hasta de 13,9% favorable a los vagones livianos. En cosecha semimecánica, realizada con trenes de vagones, disminuyó la IT al rango 60-113 Mg km ha-1; pero aumentó el pisoteo en las cabeceras hasta 39 m por surco, hubo diferencias no significativas en productividad hasta del 4% entre sistemas de vagones livianos y pesados. Además, los vagones livianos con manejo adecuado de la cosecha, llegan a ser favorables por menor consumo de combustible y emisiones.This study was carried out to determine the impact of traffic on soil compaction, crop and energy consumption during the sugarcane harvest in the Cauca river valley (Colombia. Four experiments with four replicates were harvested with different systems. Plots were mechanically harvested with self tipping, HD8000, HD12000 and HD20000 trailers and evaluated by traffic intensity (IT, direct stool traffic, penetration resistance and fuel consumption. Heavy trailers caused a greater effect due to a greater IT and direct stool traffic. IT varied between 241 and 317 Mg km ha-1. Stool traffic at the end of field varied from 8 to 18 m per furrow, meanwhile stool traffic along the furrow varied from 5 to 24%. There were no significant differences for productivity up to 13.9% favoring light trailers. Semi-mechanical harvesting was realized with trains of trailers, IT fell down to a 60 - 113 Mg km ha-1 range because a larger area is harvested during one pass of the equipment, but stool traffic increased up to 39 m per furrow for the longer trains, there was a 4% non significant difference for productivity from light to heavy trailers. Furthermore, light trailers with an adequate management are better options with lower energy consumption and emissions.

  19. Alelopatía en Saccharum spp. (caña de azúcar

    Directory of Open Access Journals (Sweden)

    R. A. Arévalo

    2011-01-01

    6 Aminoácidos no proteicos, 7 Lactonas no saturadas, 8 ácidos orgánicos, alcoholes alifáticos, aldehídos y cetonas, 9 ácidos grasos, naftoquinonas, antraquinonas, y complejos de quinonas, 10 Coumarina. Los mecanismos de acción en la mayoría de los aleloquímicos son aún desconocidos. Las plantas arvenses, como Cypro-Cyperus rotundus L.; Rooex-Rottboellia exaltata L. f. causan alelopatía en caña de azúcar. Las plantas de Cucsa- Cucumis sativus L. son afectadas por efectos alelopáticos de plantas de Rooex. Las coberturas utilizadas en la agricultura sostenible tienen propiedades alelopáticas. Las respuestas de las plantas cultivadas a los aleloquímicos pueden ser susceptibles, tolerantes o resistentes. La alelopatía tiene gran potencial para el manejo de plagas en la agricultura.

  20. Alelopatía en Saccharum spp. (caña de azúcar)

    OpenAIRE

    R. A. Arévalo; E. I. Bertoncini; E. M. Aranda; T. A. González

    2011-01-01

    El presente trabajo es una revisión de la alelopatía en la agricultura. La alelopatía incluye interacciones bioquímicas por exudados de compuestos orgánicos excretados por las plantas y otros organismos (donador) para el ambiente, las cuales provocan inhibición o estímulos en los organismos aceptores. Los aleloquímicos en los ecosistemas y agro-ecosistemas alteran el crecimiento normal de las plantas. En el reino Plantae son conocidas alrededor de 10,000 sustancias alelopáticas, a saber...

  1. Drying hard maple (Acer saccharum L.) lumber in a small dehumidification kiln

    Science.gov (United States)

    Neal. Bennett

    2013-01-01

    Portable sawmill owners quickly recognize the advantage to kiln drying lumber they produce. Having the ability to provide properly kiln-dried lumber opens new market opportunities and can increase profit margins. However, the construction and operation of a dry kiln must be economical and simple. A small dehumidification dry kiln constructed and tested in Princeton, WV...

  2. Bark thickness related to tree diameter in sugar maple (Acer saccharum Marsh.)

    Science.gov (United States)

    H. Clay Smith

    1969-01-01

    Bark thickness for sugar maple trees in Vermont was found to be related to tree diameter at breast height (d.b.h.). The relationship was positive-as the diameter increased, the bark thickness increased.

  3. Putative metabolic pathway of mannitol and sorbitol and in sugarcane Provável via metabólica de manitol e sorbitol em cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Celso Luís Marino

    2003-12-01

    Full Text Available Until the mid 1950s, boron was believed to play an important role in the transport of sugars in plants. However, boron actually depends on sugar alcohols to be taken up by the plant. In some cases, the main sugars involved in this process are sorbitol and mannitol which form stable complexes with boron. In this study, the sequences of the SugarCane EST Genome Project (SUCEST database were searched for enzymes involved in the metabolism of these sugars by comparing them with enzymes from other organisms. Eighteen contigs from sugarcane (Saccharum sp. presented high similarity with 11 enzymes involved in the putative biosynthetic pathway of sorbitol and mannitol from fructose in sugarcane. Seven of these contigs had high homology with sequences deposited in GenBank.Até meados da década de 50 acreditava-se que o boro tinha uma importante função no transporte de açúcares em plantas. Na verdade, o boro depende de açúcares álcoois para serem mobilizados dentro da planta. Em alguns organismos os principais açúcares envolvidos neste processo são o sorbitol e o manitol, que formam complexos estáveis com o micronutriente. O objetivo deste estudo foi procurar seqüências no banco de dados SugarCane EST Genome Project (SUCEST que codificam enzimas participantes na via metabólica destes açúcares através da comparação de enzimas de outros organismos. Dezoito "contigs" de cana-de-açúcar (Saccharum sp. apresentaram similaridade com onze seqüências de enzimas que compõem a provável via metabólica de sorbitol e manitol a partir de frutose. Destes "contigs", sete apresentaram uma alta similaridade entre as seqüências depositadas no GenBank.

  4. Sinopse das espécies nativas e subespontâneas de Andropogoneae Dumort. (Poaceae na Ilha de Santa Catarina, Brasil A synopsis of the Andropogoneae Dumort. (Poaceae native and subspontaneous to the Island of Santa Catarina, Florianópolis, SC, Brazil

    Directory of Open Access Journals (Sweden)

    Bárbara Toncic Neves

    2011-12-01

    Full Text Available O presente trabalho consiste no levantamento dos táxons da tribo Andropogoneae (Panicoideae - Poaceae ocorrentes na Ilha de Santa Catarina, Florianópolis, Brasil. A partir da revisão de materiais de herbários e coletas a campo foi confirmada a ocorrência de 10 gêneros e 20 espécies, 17 nativas e duas subespontâneas: Andropogon L. (7 spp., Bothriochloa Kuntze (2, Eriochrysis P. Beauv. (1, Hyparrhenia Andersson ex E. Fourn. (1, Imperata Cirillo (1, Ischaemum L. (1, Rhytachne Desv. (1, Saccharum L. (3, Schizachyrium Nees (2 e Sorghum Moench (1. Os gêneros Bothriochloa e Rhytachne são registrados pela primeira vez para a Ilha de Santa Catarina. São apresentadas chaves de identificação para gêneros e espécies, ilustrações, informações sobre distribuição geográfica, habitat, período de floração e comentários para cada táxon.The present work is a survey of the taxa in the tribe Andropogoneae (Panicoideae - Poaceae that occur on the Island of Santa Catarina, Florianópolis, Brazil. Based on a review of herbarium specimens and fieldwork, 20 species were recorded in the following 10 genera: Andropogon L. (7 spp., Bothriochloa Kuntze (2, Eriochrysis P. Beauv. (1, Hyparrhenia Andersson ex E. Fourn. (1, Imperata Cirillo (1, Ischaemum L. (1, Rhytachne Desv. (1, Saccharum L. (3, Schizachyrium Nees (2 and Sorghum Moench (1. Seventeen of the species are native, and two species are subspontaneous. Bothriochloa and Rhytachne are recorded here, for the first time, for the Island of Santa Catarina. This work provides key to the genera and species, as well as illustrations, information about geographic distribution, habitat, phenology, and comments about each taxon presented in the study.

  5. Levantamento da incidência de Leifsonia xyli subsp. xyli em plantios de cana-de-açúcar do noroeste do Paraná Survey of Leifsonia xyli subsp. xyli incidence in sugarcane plantations of the northwest region of Paraná State

    Directory of Open Access Journals (Sweden)

    Fernanda Santos Marcuz

    2009-01-01

    Full Text Available O raquitismo-da-soqueira, causado por Leifsonia xyli subsp. xyli é considerado uma das mais importantes doenças bacterianas da cana-de-açúcar (Saccharum spp.. Para verificar a possível ocorrência de Leifsonia xyli subsp. xyli na região noroeste do Paraná, foi realizado um levantamento em 32 áreas de plantio de duas usinas, utilizando-se o método sorológico "Dot blot" e um protocolo baseado na reação em cadeia da polimerase. As amostras foram coletadas em áreas cultivadas com 8 variedades e 2 clones, a partir de plantas em diferentes épocas de corte, perfazendo um total de 1395 amostras. Constatou-se a presença da bactéria no fluído fibrovascular de plantas da variedade SP77-5181 coletado em uma das propriedades, com índice de incidência de 23%. Esses resultados indicam a incidência de Leifsonia xyli subsp. xyli no noroeste do Paraná.Ratoon stunting disease caused by Leifsonia xyli subsp. xyli is one of the most important bacterial diseases of sugarcane (Saccharum spp.. A ratoon stunting desease survey was carried in 32 sugarcane producing areas from two mills in the northwest region of Paraná State through a dot blot serological protocol and a polymerase chain reaction-based assay. A total of 1395 samples were collected in areas planted with 8 varieties and 2 sugarcane clones collected from plants at different harvest periods. The bacterium was detected in the fibrovascular fluid of the SP77-5181 variety from a producing area in a 23% rate of incidence. These data indicated that Leifsonia xyli subsp. xyli is present in the northwest region of Paraná.

  6. Role of motility and chemotaxis in the pathogenesis of Dickeya dadantii 3937 (ex Erwinia chrysanthemi 3937).

    Science.gov (United States)

    Antúnez-Lamas, María; Cabrera-Ordóñez, Ezequiel; López-Solanilla, Emilia; Raposo, Rosa; Trelles-Salazar, Oswaldo; Rodríguez-Moreno, Andrés; Rodríguez-Palenzuela, Pablo

    2009-02-01

    Dickeya dadantii 3937 (ex Erwinia chrysanthemi), a member of the Enterobacteriaceae, causes soft rot in many economically important crops. A successful pathogen has to reach the interior of the plant in order to cause disease. To study the role of motility and chemotaxis in the pathogenicity of D. dadantii 3937, genes involved in the chemotactic signal transduction system (cheW, cheB, cheY and cheZ) and in the structure of the flagellar motor (motA) were mutagenized. All the mutant strains grew like the wild-type in culture media, and the production and secretion of pectolytic enzymes was not affected. As expected, the swimming ability of the mutant strains was reduced with respect to the wild-type: motA (94%), cheY (80%), cheW (74%), cheB (54%) and cheZ (48%). The virulence of the mutant strains was analysed in chicory, Saintpaulia and potato. The mutant strains were also tested for their capability to enter into Arabidopsis leaves. All the mutants showed a significant decrease of virulence in certain hosts; however, the degree of virulence reduction varied depending on the virulence assay. The ability to penetrate Arabidopsis leaves was impaired in all the mutants, whereas the capacity to colonize potato tubers after artificial inoculation was affected in only two mutant strains. In general, the virulence of the mutants could be ranked as motA

  7. Sugarcane-Biorefinery.

    Science.gov (United States)

    Vaz, Sílvio

    2017-03-17

    Concepts such as biorefinery and green chemistry focus on the usage of biomass, as with the oil value chain. However, it can cause less negative impact on the environment. A biorefinery based on sugarcane (Saccharum spp.) as feedstock is an example, because it can integrate into the same physical space, of processes for obtaining biofuels (ethanol), chemicals (from sugars or ethanol), electricity, and heat.The use of sugarcane as feedstock for biorefineries is dictated by its potential to supply sugars, ethanol, natural polymers or macromolecules, organic matter, and other compounds and materials. By means of conversion processes (chemical, biochemical, and thermochemical), sugarcane biomass can be transformed into high-value bioproducts to replace petrochemicals, as a bioeconomy model.

  8. Environmental factors affecting sporulation of Fuligo septica (Myxomycetes on sugar cane bagasse

    Directory of Open Access Journals (Sweden)

    Chiappeta Alda de Andrade

    2003-01-01

    Full Text Available The influence of environmental factors on sporulation of Fuligo septica (L. Wigg. and the abundance of this species on sugar cane bagasse (Saccharum officinarum L., stored outdoors was studied.In Northeastern Brazil, between January/1997 and January/1998, a total of 29 specimens were collected through monthly collections of aethalia. The relationships between the abundance of aethalia and rainfall, temperature, relative humidity of the air and insolation were studied. Results indicated that on the substrate analyzed, F. septica was an abundant species. Sporulation occurred in all seasons of the year, with a well-defined peak at the end of winter and beginning of spring (August/September,which was strongly influenced by rainfall.

  9. Avaliação da divergência nutricional de genótipos de cana-de-açúcar (Saccharum spp. Evaluation of the nutritional divergence of sugarcane (Saccharum spp. genotypes

    Directory of Open Access Journals (Sweden)

    Acyr Wanderley de Paula Freitas

    2006-02-01

    Full Text Available Objetivou-se neste trabalho avaliar a divergência nutricional de genótipos de cana-de-açúcar para uso na alimentação de animais ruminantes. Foram avaliados os genótipos: RB72454, RB835486, SP80-1842, IAC86-2480, RB977512, RB867515, RB935566, RB925345, RB977625, SP79-1011, SP80-1816, SP81-3250 e SP91-1049, colhidos em soca aos 11 meses de idade, quanto às variáveis FDN, hemicelulose, lignina, relação FDN x carboidratos solúveis, fração indegradável da FDN, taxa de degradação da FDN e taxa de degradação da matéria seca. A aplicação da análise de agrupamento segundo o método de Tocher, utilizando a matriz de distâncias euclidiana média, permitiu o estabelecimento de três grupos de genótipos. A FDN e a taxa de degradação da MS foram as variáveis de maior importância para a discriminação dos grupos, contribuindo cada uma com 25,64% do poder de discriminação total. A fração indegradável da FDN foi a variável que apresentou a menor contribuição para discriminação dos grupos (11,54%. A variáveis FDN, fração indegradável da FDN, hemicelulose, taxa de degradação da MS e taxa de degradação da FDN mostraram-se eficazes para o estudo da diversidade nutricional da cana-de-açúcar. O grupo III, constituído pelo clone RB977512, apresentou as melhores médias para as variáveis avaliadas e, portanto, pode ser considerado de melhor qualidade nutricional para alimentação de ruminantes.The objective of this trial was to study the nutritional divergence of sugarcane genotypes for feeding ruminants. The following sugarcane genotypes were evaluated: RB72454, RB835486, SP80-1842, IAC86-2480, RB977512, RB867515, RB935566, RB925345, RB977625, SP79-1011, SP80-1816, SP81-3250, and SP91-1049 all harvested at 11 months of age. The discriminatory variables were: neutral detergent fiber (NDF, hemicellulose, lignin, NDF to soluble carbohydrate ratio, NDF undegradable fraction, and rates of NDF and DM degradation. Application of the cluster analyses according to the Tocher approach, using the mean Euclidian distance matrix, yielded three groups of genotypes. Neutral detergent fiber and DM degradation rates were the most important variables for group discrimination contributing individually with 25.64% of the total discrimination power while the least contribution was observed with NDF undegradable fraction (11.54%. The variables NDF, NDF undegradable fraction, hemicellulose, and rates of DM and NDF degradation all showed to be effective to evaluate sugarcane nutritional divergence. Group III formed by the clone RB977512 showed the best responses for the studied variables and as such can be considered of higher nutritional quality for ruminants.

  10. Facile synthesis of water-soluble carbon nano-onions under alkaline conditions

    Directory of Open Access Journals (Sweden)

    Gaber Hashem Gaber Ahmed

    2016-05-01

    Full Text Available Carbonization of tomatoes at 240 °C using 30% (w/v NaOH as catalyst produced carbon onions (C-onions, while solely carbon dots (C-dots were obtained at the same temperature in the absence of the catalyst. Other natural materials, such as carrots and tree leaves (acer saccharum, under the same temperature and alkaline conditions did not produce carbon onions. XRD, FTIR, HRTEM, UV–vis spectroscopy, and photoluminescence analyses were performed to characterize the as-synthesized carbon nanomaterials. Preliminary tests demonstrate a capability of the versatile materials for chemical sensing of metal ions. The high content of lycopene in tomatoes may explain the formation of C-onions in alkaline media and a possible formation mechanism for such structures was outlined.

  11. Lignin-Furfural Based Adhesives

    Directory of Open Access Journals (Sweden)

    Prajakta Dongre

    2015-07-01

    Full Text Available Lignin recovered from the hot-water extract of sugar maple (Acer saccharum is used in this study to synthesize adhesive blends to replace phenol-formaldehyde (PF resin. Untreated lignin is characterized by lignin content and nuclear magnetic resonance (NMR analysis. The molecular weight distribution of the lignin and the blends are characterized by size exclusion chromatography (SEC. The effect of pH (0.3, 0.65 and 1, ex situ furfural, and curing conditions on the tensile properties of adhesive reinforced glass fibers is determined and compared to the reinforcement level of commercially available PF resin. The adhesive blend prepared at pH = 0.65 with no added furfural exhibits the highest tensile properties and meets 90% of the PF tensile strength.

  12. ISOLASI cDNA SUCROSE TRANSPORTER (SUT DARI BATANG TANAMAN TEBU (Saccharum officinarum L.

    Directory of Open Access Journals (Sweden)

    - Slameto

    2010-09-01

    Full Text Available Sucrose Transporter (SUT is kind of protein transporter that control in sucrose translocation. Sucrose Transporter is intermediate in translocation of sucrose from apoplasmic to simplasmic. SUT facilitates sucrose transportation from vascular tissues to parenchyma cells toward in node sugarcane stem. This research was purposed to isolate cDNA SUT from sugarcane stem, and cloned in Escherichia coli strain DH5α. Total RNA of sugarcane stem was isolated by single step method, then add with oligo dT in order to obtain the first strand of SUT cDNA then used as template for PCR. The primer used for PCR is 5’ –ggg ctg att gtg gcc atg tc- ‘3 (SUT-F and 5’ –tgc cct ttg tct ccg gaa cc- ‘3 (SUT-R. PCR was programmed as follow denaturation at 94°C for 2 minutes and 30 second, annealing at 54°C for 30 s, extension at 72°C 2 min and 7 min, and storage at 4°C for unlimited, It was for 30 cycles. Complementary DNA SUT from PCR ligalized to pTOPO bunt-end, then it cloned in to E. coli strain DH5α. The cloning resulted then be sequenced in order to observe the homologues with other nucleotides sequences of some plant using BLASTn program in GENE BANK NCBI and the level of homology determined by Genetyx program. The concentrated of total RNA isolated was 5,024 μg/μl, with purity of 1,85. Complementary DNA SUT fragment from PCR with size 2037 bp appropriated to the both of primer was used. Complementary DNA SUT fragment showed by analyzed some of restriction enzyme e.g. EcoRI, PstI and BamHI. Homologues of this cDNA SUT fragment was 100% to SoSUT 2A of sugarcane stem and 84% to OsSUT of rice plant (Casu et al ., 2003.

  13. Present situation concerning studies on associative n-fixation in sugarcane, 'Saccharum officinarum' L

    International Nuclear Information System (INIS)

    Ruschel, A.P.; Vose, P.B.

    1977-01-01

    High nitrogenase activity in sugarcane has been confirmed, using 15 N procedure, which indicated rapid translocation of the fixed nitrogen to the plant tissue. It is observed that there are differences in variety concerning nitrogenase activity in the rhizosphere of sugarcane. Basic calculations and an evaluation of possible biological N-fixation with 15 N suggest that possibly as much as 25-30% of the plant nitrogen may be derived from biological fixation. Both eight and electron microscope studies suggest that there are bacteria inside healthy roots and if such a root is surface sterilized and placed ou nitrogen-free media then bacteria stream from the cut surface [pt

  14. Evaluación de ensilajes mixtos de Saccharum officinarum y Gliricidia sepium con la utilización de aditivos Evaluation of mixed silages of Saccharum officinarum and Gliricidia sepium using additives

    Directory of Open Access Journals (Sweden)

    R Suárez

    2011-03-01

    Full Text Available Se realizó un experimento en Trujillo, Venezuela, con el objetivo de evaluar el efecto de la adición de melaza y urea en ensilajes de cogollo quemado de caña de azúcar y Gliricidia sepium, utilizando macrobolsas de 1,18 m³ de capacidad y vacío, mediante un diseño factorial 4 (tipo de ensilaje x 3 (tiempo de fermentación: 20, 40 y 60 días y cinco réplicas. Los tratamientos fueron T1 (cogollo de caña de azúcar, 75% más G. sepium, 25%; T2 (T1 más urea, 0,5%; T3 (T1 más melaza, 4% y T4 (T3 más urea, 0,5%. Se determinó el pH, la MS, la PB, el amoníaco, el nitrógeno soluble, la FB, el extracto libre de nitrógeno, el extracto etéreo, el Ca, el P y la ceniza. No hubo interacción tipo de ensilaje por tiempo de fermentación. Con la adición de urea (T2 se observaron los mayores valores de pH (5,03, PB (8,27%, NH3/Nt (18,13% y NS/Nt (38,31%. Al adicionar la melaza (T3 se constató un mayor porcentaje de MS (35,34 y de ELN (50,93, y con los dos aditivos (T4 se observó un menor contenido de EE (1,02%. Los tenores de FB, los minerales y la ceniza no presentaron diferencias entre los tratamientos. A los 40 días de fermentación se observó un mayor contenido de MS (44,80% y ELN (49,72%. Sin embargo, a los 60 días se obtuvo mayor relación NH3/Nt (11,17% y NS/Nt (33,61%. Mediante la ponderación de las variables más relevantes se concluyó que los ensilajes de mayor calidad se obtuvieron cuando sólo se adicionó melaza (4% o se combinó con urea (0,5%; mientras que la calidad del material conservado fue menor a los 60 días de elaboración.A trial was conducted in Trujillo, Venezuela, in order to evaluate the effect of the addition of molasses and urea in silages of burnt sugarcane tops and Gliricidia sepium, using macrobags of capacity 1,18 m³ and vacuum, by means of a 4 (type of silage x 3 (fermentation time: 20, 40 and 60 days factorial arrangement and five replications. The treatments were T1 (sugarcane tops, 75% plus G. sepium, 25%; T2 (T1 plus urea, 0,5%; T3 (T1 plus molasses, 4% and T4 (T3 plus urea, 0,5%. The following indicators were determined: pH, DM, CP, ammonia, soluble nitrogen, CF, nitrogen-free extract, ether extract, Ca, P and ash. There was no silage type by fermentation time interaction. With the addition of urea (T2 the highest values of pH (5,03, CP (8,27%, NH3/Nt (18,13% and SN/Nt (38,31% were observed. With the addition of molasses (T3 a higher percentage of DM (35,34 and NFE (50,93 was detected, and with the two additives (T4 a lower content of EE (1,02% was obtained. The CF, mineral and ash values did not show differences among treatments. After 40 days of fermentation a higher content of DM (44,80% and NFE (49,72% was observed. However, after 60 days a higher NH3/Nt (11,17% and SN/Nt (33,61% ratio was obtained. By pondering the most relevant variables, it was concluded that the best quality silages were obtained when adding only molasses (4% or when it was combined with urea (0,5%; while the quality of the preserved material was lower 60 days after being elaborated.

  15. Development, cross-species/genera transferability of novel EST-SSR markers and their utility in revealing population structure and genetic diversity in sugarcane

    KAUST Repository

    Singh, Ram K.

    2013-07-01

    Sugarcane (Saccharum spp. hybrid) with complex polyploid genome requires a large number of informative DNA markers for various applications in genetics and breeding. Despite the great advances in genomic technology, it is observed in several crop species, especially in sugarcane, the availability of molecular tools such as microsatellite markers are limited. Now-a-days EST-SSR markers are preferred to genomic SSR (gSSR) as they represent only the functional part of the genome, which can be easily associated with desired trait. The present study was taken up with a new set of 351 EST-SSRs developed from the 4085 non redundant EST sequences of two Indian sugarcane cultivars. Among these EST-SSRs, TNR containing motifs were predominant with a frequency of 51.6%. Thirty percent EST-SSRs showed homology with annotated protein. A high frequency of SSRs was found in the 5\\'UTR and in the ORF (about 27%) and a low frequency was observed in the 3\\'UTR (about 8%). Two hundred twenty-seven EST-SSRs were evaluated, in sugarcane, allied genera of sugarcane and cereals, and 134 of these have revealed polymorphism with a range of PIC value 0.12 to 0.99. The cross transferability rate ranged from 87.0% to 93.4% in Saccharum complex, 80.0% to 87.0% in allied genera, and 76.0% to 80.0% in cereals. Cloning and sequencing of EST-SSR size variant amplicons revealed that the variation in the number of repeat-units was the main source of EST-SSR fragment polymorphism. When 124 sugarcane accessions were analyzed for population structure using model-based approach, seven genetically distinct groups or admixtures thereof were observed in sugarcane. Results of principal coordinate analysis or UPGMA to evaluate genetic relationships delineated also the 124 accessions into seven groups. Thus, a high level of polymorphism adequate genetic diversity and population structure assayed with the EST-SSR markers not only suggested their utility in various applications in genetics and genomics in

  16. Vertical leaf mass per area gradient of mature sugar maple reflects both height-driven increases in vascular tissue and light-driven increases in palisade layer thickness.

    Science.gov (United States)

    Coble, Adam P; Cavaleri, Molly A

    2017-10-01

    A key trait used in canopy and ecosystem function modeling, leaf mass per area (LMA), is influenced by changes in both leaf thickness and leaf density (LMA = Thickness × Density). In tall trees, LMA is understood to increase with height through two primary mechanisms: (i) increasing palisade layer thickness (and thus leaf thickness) in response to light and/or (ii) reduced cell expansion and intercellular air space in response to hydrostatic constraints, leading to increased leaf density. Our objective was to investigate within-canopy gradients in leaf anatomical traits in order to understand environmental factors that influence leaf morphology in a sugar maple (Acer saccharum Marshall) forest canopy. We teased apart the effects of light and height on anatomical traits by sampling at exposed and closed canopies that had different light conditions at similar heights. As expected, palisade layer thickness responded strongly to cumulative light exposure. Mesophyll porosity, however, was weakly and negatively correlated with light and height (i.e., hydrostatic gradients). Reduced mesophyll porosity was not likely caused by limitations on cell expansion; in fact, epidermal cell width increased with height. Palisade layer thickness was better related to LMA, leaf density and leaf thickness than was mesophyll porosity. Vein diameter and fraction of vascular tissue also increased with height and LMA, density and thickness, revealing that greater investment in vascular and support tissue may be a third mechanism for increased LMA with height. Overall, decreasing mesophyll porosity with height was likely due to palisade cells expanding into the available air space and also greater investments in vascular and support tissue, rather than a reduction of cell expansion due to hydrostatic constraints. Our results provide evidence that light influences both palisade layer thickness and mesophyll porosity and indicate that hydrostatic gradients influence leaf vascular and support

  17. Phylogenetic reconstruction using four low-copy nuclear loci strongly supports a polyphyletic origin of the genus Sorghum.

    Science.gov (United States)

    Hawkins, Jennifer S; Ramachandran, Dhanushya; Henderson, Ashley; Freeman, Jasmine; Carlise, Michael; Harris, Alex; Willison-Headley, Zachary

    2015-08-01

    Sorghum is an essential grain crop whose evolutionary placement within the Andropogoneae has been the subject of scrutiny for decades. Early studies using cytogenetic and morphological data point to a poly- or paraphyletic origin of the genus; however, acceptance of poly- or paraphyly has been met with resistance. This study aimed to address the species relationships within Sorghum, in addition to the placement of Sorghum within the tribe, using a phylogenetic approach and employing broad taxon sampling. From 16 diverse Sorghum species, eight low-copy nuclear loci were sequenced that are known to play a role in morphological diversity and have been previously used to study evolutionary relationships in grasses. Further, the data for four of these loci were combined with those from 57 members of the Andropogoneae in order to determine the placement of Sorghum within the tribe. Both maximum likelihood and Bayesian analyses were performed on multilocus concatenated data matrices. The Sorghum-specific topology provides strong support for two major lineages, in alignment with earlier studies employing chloroplast and internal transcribed spacer (ITS) markers. Clade I is composed of the Eu-, Chaeto- and Heterosorghum, while clade II contains the Stipo- and Parasorghum. When combined with data from the Andropogoneae, Clade II resolves as sister to a clade containing Miscanthus and Saccharum with high posterior probability and bootstrap support, and to the exclusion of Clade I. The results provide compelling evidence for a two-lineage polyphyletic ancestry of Sorghum within the larger Andropogoneae, i.e. the derivation of the two major Sorghum clades from a unique common ancestor. Rejection of monophyly in previous molecular studies is probably due to limited taxon sampling outside of the genus. The clade consisting of Para- and Stiposorghum resolves as sister to Miscanthus and Saccharum with strong node support. © The Author 2015. Published by Oxford University Press on

  18. EFECTO DE LA BIOFERTILIZACIÓN SOBRE EL CRECIMIENTO EN MACETA DE PLANTAS DE CAÑA DE AZÚCAR (Saccharum officinarum EFEITO DA BIOFERTILIZAÇÃO NO CRESCIMENTO DE PLANTAS EM VASOS açúcar de cana (Saccharum officinarum EFFECT OF BIOFERTILIZATION ON THE GROWTH OF POTTED SUGARCANE PLANTS (Saccharum officinarum

    Directory of Open Access Journals (Sweden)

    LILIANA SERNA-COCK

    2011-12-01

    Full Text Available El uso de microorganismos como fertilizante, ha demostrado tener efectos benéficos sobre el crecimiento de plantas y son una alternativa al uso de fertilizantes guímicos, sin embargo, cada microorganismo difiere en sus efectos benéficos. En este trabajo se evaluó el efecto de la aplicación de microorganismos fertilizantes, Azospirillum brasilense, Azotobacter chroccocum y Trichoderma lignorum sobre el crecimiento en maceta de plantas de caña de azúcar variedad CC 934418. El crecimiento de las plantas se midió en términos de diámetro del tallo, longitud de tallo y raíces, y número de hojas y raíces a los 15, 30 y 45 días de la siembra. El crecimiento de las plantas mostró diferencias estadísticamente significativas entre los tratamientos. Los microorganismos fertilizantes mostraron efecto positivo sobre el crecimiento de plantas de caña de azúcar, siendo Azospirillum brasilense y Trichoderma lignorum los microorganismos gue ejercieron mayor efecto sobre el diámetro del tallo y los sistemas radical y foliar de la planta. Se observaron los efectos beneficiosos de Trichoderma lignorum sobre el crecimiento de la hoja. Este es un nuevo aporte científico, ya que esta especie no ha sido reportada como promotora de crecimiento vegetal.0 uso de microrganismos como fertilizante, tem sido demonstrado gue têm efeitos benéficos no crescimento das plantas e são considerados uma alternativa ao uso de fertilizantes guímicos, no entanto, cada microrganismo possui diferentes efeitos benéficos. Neste estudo foi avaliado o efeito da aplicação de microorganismos fertilizantes, Azospirillum brasilense, Azotobacter chroccocum e Trichoderma lignorum no crescimento de cana-de-açucar da variedade CC 934418 plantadas em vasos. 0 crescimento das plantas foi medido em termos do diâmetro do caule, comprimento de caule e da raiz e número de folhas e raízes nos dias 15, 30 e 45 após a semeadura. 0 crescimento da planta mostrou diferenças significativas entre os tratamentos. Os microrganismos fertilizantes mostraram efeito positivo sobre o crescimento das plantas de cana, os microrganismos Azospirillum brasilense e Trichoderma lignorum exerceram um efeito maior em diâmetro do caule, sistema radicular e folhas da planta. Os efeitos benéficos do Trichoderma em lignorum crescimento da folha foram observadas. Esta é uma nova contribuição científica vez que esta espécie não foi relatada como a promoção de crescimento vegetal.The use of microorganisms as fertilizer has demonstrated beneficial effects on plant growth and is an alternative to chemical fertilizers. However, each microorganism has different beneficial effects. This study evaluated the effect of applying microorganism fertilizers, Azospirillum brasilense, Azotobacter chroccocum, and Trichoderma lignorum on the growth of potted sugarcane plants var. CC 934418. Plant growth was measured in terms of stem diameter, stem and root length, and the number of leaves and roots 15, 30, and 45 days after planting. Plant growth evidenced statistically significant differences among treatments. Microorganism fertilizers showed a positive effect on the growth of sugarcane plants, with Azospirillum brasilense and Trichoderma lignorum as the microorganisms that exercised the greatest effect on stem diameter, root systems, and plant foliation. Beneficial effects of Trichoderma lignorum on leaf growth were observed. This is a new scientific contribution since this species has not been reported as promoting plant growth.

  19. Contribuição da cadeia produtiva da cana-de-açúcar (Saccharum officinarum L. no sabor dos alimentos / Contribution of sugar-cane (Saccharum officinarum L. value chain in food flavour

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    Romeu Schvarz Sobrinho

    2010-04-01

    Full Text Available Muitos alimentos não seriam palatáveis se não fosse à aplicação do glutamato monossódico como ingrediente alimentar. Após a descoberta do quinto sabor (sabor umami, indústrias se estabeleceram para produzi-lo e outras passaram a utilizá-lo como aditivo realçador do sabor. Amparado nesses fatos, este estudo tem por objetivo destacar a importância do glutamato monossódico na palatabilidade humana para consumo de produtos industrializados, no uso da cana-de-açúcar como matéria-prima (substrato para produção do glutamato monossódico e na utilização deste pela empresa Perdigão Agroindustrial. Propõe-se a responder a seguinte questão: a como os produtos industrializados da empresa Perdigão Agroindustrial se beneficiam da cadeia produtiva da cana-de-açúcar e do glutamato monossódico? Para responder esta questão, fez-se necessário, inicialmente, responder às seguintes perguntas: a como a cadeia produtiva da cana-de-açúcar contribui para a produção de glutamato monossódico?; e, b qual a importância deste realçador de sabor na palatabilidade humana? Por meio de pesquisa bibliográfica de fontes secundárias, foi possível identificar que a cadeia produtiva da cana-de-açúcar contribui com a indústria de alimentos, fornecendo matéria-prima para produção de glutamato monossódico. Este produto, por sua vez, é utilizado na indústria de alimentos prontos ou semiprontos como carne bovina, suína e de aves e outras comidas rápidas e congeladas. AbstractMost of food would not be palatable if there were not an addition of mono sodium glutamate as a food ingredient. Thus, after the discovery of the fifth flavor (umami flavour, industries were established in order to produce it while the other industries use this flavour as food highlighter additive. Based on these facts, the aim of this study is to emphasize the importance of mono sodium glutamate in human palatability for consumption of industrialized products, and in the use of sugar-cane as raw material for production of mono sodium glutamate and in its use by Perdigão Agroindustrial enterprise. Hence, the present article attempts to answer the following question: In which way does the industrialized productsof Perdigão Agroindustrial. enterprise benefit from the sugar-cane value chain and from the mono sodium glutamate? In order reply to this question it was first necessary to answer the following questions: a How did sugar-cane value chain contribute for the production of mono sodium glutamate? b What is the importance of this flavour highlighter in human palatability? From bibliography research on secondary sources, it was possible to identify that the sugar-cane value chain has contributed in food industry providing sugar-cane and molasses as raw material for mono sodium glutamate production. This product, in turn, is used in the industry of ready or semi-ready food like beef, pork and poultry, as well as other frozen and fast food.Key words: mono sodium glutamate; umami flavour; Perdigão Agroindustrial; product innovation.

  20. Orange rust in sugarcane: molecular identification in Rio de Janeiro State

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    Carla Vanessa Borges Castro

    2013-09-01

    Full Text Available Sugarcane (Saccharum spp. cultivation is one of the major agricultural activities in the Brazilian states. This study aimed to molecularly identify the pathogen associated with rust in sugarcane cultivars in the state of Rio de Janeiro and to suggest a control strategy. Among the 14 PCR-tested cultivars, Puccinia kuehnii infection was identified for RB947520, RB92606, RB835486, RB72454, SP89-11I5, SP83-2847, both from infected leaf sample and from urediniospores. Puccinia kuehnii was not detected by PCR for the cultivars RB955971, RB951541, RB92579, RB867515, RB855536, SP91-1049, SP80-3280, SP80-1816. This is the first molecular detection of this fungus in the state of Rio de Janeiro for six of the 14 analyzed cultivars.

  1. Genetic diversity analysis of chewing sugarcane (Saccharum officinarum L. varieties by using RAPD markers

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    S. M. Sarid Ullah

    2013-01-01

    Full Text Available In the present study an efficient and easy method was followed for the isolation of DNA from meristem cylinder in five chewing sugarcane varieties, namely Amrita, Bomaby, Babulal (Co.527, Q83 and Misrimala. The quality and quantity of DNA were assured by visual estimation using agarose gel electrophoresis and UV spectrophotometry. The highest amount of DNA was retrieved from the Amrita (3250 ng/ml and the lowest amount was attained from the variety Q83 (1450 ng/ml. The amount of recovered DNA was enough for PCR amplification and marker studies such as random amplified polymorphic DNA (RAPD. Using RAPD markers, bands obtained from fingerprinting (190 bp to 1200 bp showed 73.5% polymorphism. The dendrogram, based on linkage distance using unweighted pair group method of arithmetic means (UPGMA, indicated segregation of the five chewing varieties of sugarcane into two main clusters. Amrita, Bombay and Misrimala were grouped in cluster 1 (C1 followed by sub-clusters. Babulal and Q83 were grouped in cluster 2 (C2. The results of the present investigation also revealed that the twenty RAPD primers were able to identify and classify the chewing sugarcane varieties based on their genetic relationship.

  2. Growth Indicators of a 48-Clone Sugar Cane Population (Saccharum spp. with Forage Potential

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    Yoslen Fernández Gálvez

    2016-09-01

    Full Text Available The aim of this paper was to determine growth indicators in a 48-clone sugar cane population, with promising phenotypical features for forage production. The following indicators were assessed: leaf area (A, leaf area index (LA1; leaf area ratio (LAR; specific leaf area (SLA; leaf weight ratio (LWR; crop growth rate (CGR; net assimilation rate (NAR; relative growth rate in weight (RGR; biomass production speed (G; leaf area duration (LAD; and biomass duration (Z, monthly (187 - 370 days. The minimum, the mean, the maximum values, and the population variance were determined for all cutting ages and the variables assessed. The results achieved have provided quantitative values that can be used as reference for selection and assessment of forage genotypes for ruminant nutrition.

  3. Foliar fertilization of sugarcane (Saccharum spp): absorption and translocation of 15-N-labeled urea

    International Nuclear Information System (INIS)

    Trivelin, P.C.O.; Carvalho, J.G. de; Silva, A.Q. da; Primavesi, A.C.P.A.; Camacho, E.; Eimori, I.E.; Guilherme, M.R.

    1988-01-01

    The absorption and translocation of foliar applied nitrogen as urea solution to sugar cane plants was evaluated. An experiment using the isotope dilution technique with 15 N labeled urea was carried out in green house condition. Seedlings of sugarcane variety IAC 53-150 were planted in pots with 5KG of top soil''latossolo vermelho amarelo, fase arenosa'' (Haplustox). (M.A.C.) [pt

  4. Macro-microscopic anatomy: obtaining a composite view of barrier zone formation in Acer saccharum

    Science.gov (United States)

    Kenneth Dudzik

    1988-01-01

    The technique for constructing a montage of large wood sections cut on a sliding microtome is discussed. Briefly, the technique involves photographing many serial micrographs in a pattern under a light microscope similar to the way flight lines are run in aerial photography. Assembly of the resulting overlapping photographs requires careful trimming. A composite of...

  5. Antioxidant and Nitrite-Scavenging Capacities of Phenolic Compounds from Sugarcane (Saccharum officinarum L. Tops

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    Jian Sun

    2014-08-01

    Full Text Available Sugarcane tops were extracted with 50% ethanol and fractionated by petroleum ether, ethyl acetate (EtOAc, and n-butyl alcohol successively. Eight phenolic compounds in EtOAc extracts were purified through silica gel and Sephadex LH-20 column chromatographies, and then identified by nuclear magnetic resonance and electrospray ionization mass spectra. The results showed that eight phenolic compounds from EtOAc extracts were identified as caffeic acid, cis-p-hydroxycinnamic acid, quercetin, apigenin, albanin A, australone A, moracin M, and 5'-geranyl-5,7,2',4'-tetrahydroxyflavone. The antioxidant and nitrite-scavenging capacities of different solvent extracts correlated positively with their total phenolic (TP contents. Amongst various extracts, EtOAc extracts possessed the highest TP content and presented the strongest oxygen radical absorbance capacity (ORAC, 1,1'-diphenyl-2-picrylhydrazyl (DPPH radical-scavenging capacity, 2,2'-azobis-3-ethylbenthiaazoline-6-sulfonic acid (ABTS radical-scavenging capacity, ferric reducing antioxidant power (FRAP and nitrite-scavenging capacity. Thus, sugarcane tops could be promoted as a source of natural antioxidant.

  6. Intraspecific diversity in Sinningia speciosa (Gesneriaceae: Sinningieae), and possible origins of the cultivated florist's gloxinia

    Science.gov (United States)

    Zaitlin, David

    2012-01-01

    Background and aims The florist's gloxinia is a familiar houseplant in the Gesneriaceae, the botanical family that includes the African violet (Saintpaulia) and other ornamental species. The gloxinia's wild progenitor is Sinningia speciosa (Lodd.) Hiern, a Brazilian endemic. Although it has been cultivated for almost 200 years, little is known about the genetic diversity in S. speciosa, how the wild populations relate to one another or even where the cultivated forms originated. Using available wild collections, preliminary phenetic and phylogenetic investigations were conducted to elucidate the interspecific relationships within S. speciosa and to infer the origins of the cultivars. Methodology Amplified fragment length polymorphism (AFLP) analysis was applied to 24 accessions of S. speciosa (17 wild collections, seven cultivars) and one accession each of Sinningia guttata and Sinningia macrophylla. A maximum likelihood (ML) tree was also calculated from an alignment of the nuclear ribosomal internal transcribed spacer sequence from the same 26 accessions. Principal results Dice/UPGMA and principal coordinates analysis of the AFLP data partitioned S. speciosa into several distinct clusters, one of which included S. macrophylla. All cultivated ‘gloxinias’ grouped together in a major cluster with plants from Rio de Janeiro. The AFLP results were compared with a phylogenetic analysis of the ribosomal spacer region, which was informative in S. speciosa. The ML tree generally supported the AFLP results, although several clades lacked strong statistical support. Conclusions Independent analyses of two different data sets show that S. speciosa is a diverse species comprised of several lineages. Genetic distance estimates calculated from the AFLP data were positively correlated with geographic distances between populations, indicating that reproductive isolation could be driving speciation in this taxon. Molecular markers are under development for population genetic

  7. The characteristics of bioethanol fuel made of vegetable raw materials

    Science.gov (United States)

    Muhaji; Sutjahjo, D. H.

    2018-01-01

    The aim of this research is to identify the most potential vegetable raw as the material to make a bioethanol fuel as the alternative energy for gasoline. This study used experimental method. The high-level bioethanol was obtained through the process of saccharification, fermentation and stratified distillation. ASTM standards were used as the method of testing the chemical element (D 5501, D 1744, D 1688, D 512, D 2622, D 381), and physical test (D 1613, D 240, D 1298-99, D 445, and D 93). The result of the analysis showed that from the seven bioethanols being studied there is one bioethanol from Saccharum of icinarum linn that has physical and chemical properties close to the standard of bioethanol. Meanwhile, the others only meet some of the physical and chemical properties of the standard bioethanol.

  8. Biosorption treatment of brackish water

    International Nuclear Information System (INIS)

    Rizwan, M.; Ali, M.; Tariq, M.I.; Rehman, F.U.; Karim, A.; Makshoof, M.; Farooq, R.

    2010-01-01

    Biosorptivity of different agricultural wastes have been evaluated for the treatment of brackish water and a new method, based on the principle of bio-sorption has been described. Wastes of the Saccharum officinarum, Moringa oleifera, Triticum aestivcum and Oryza sativa have been used in raw forms as well as after converting them into ash and activated carbon as biosorbents for treatment of brackish water in this study. Samples of brackish water have been analyzed before and after treatment for quality control parameters of water. A significant Improvement has been observed in quality control parameters of water after treatment. pH of the water samples slightly increased from 7.68 to 7.97 with different treatments. A substantial decrease in conductivity,. TDS, TH, concentrations of cations and anions was observed in the samples of brackish water after treatment with different biosorbents. (author)

  9. Maturation curves of sweet sorghum genotypes

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    Renan Silva e Souza

    2016-02-01

    Full Text Available ABSTRACT Sweet sorghum [Sorghum bicolor (L. Moench] stands out as a complementary crop to sugarcane Saccharum spp. for the production of ethanol, since it has juicy stems with directly fermentable sugars. Due to this fact, there is a need for the analysis of sweet sorghum properties in order to meet the agro-industry demand. This work aimed to develop and study the maturation curves of seven sweet sorghum cultivars in ten harvest dates. The results showed a significant difference between cultivars and harvest dates for all parameters analysed (p≤0.01. Regarding the sugar content, the cultivars BRS508, XBWS80147 and CMSX629 showed the highest means for the total reducing sugars (TRS and recoverable sugar (RS. In the production of ethanol per tonne of biomass (EP, the cultivars BRS508 and CMSX629 presented the best results.

  10. Vermicomposting and anaerobic digestion – viable alternative options for terrestrial weed management – A review

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    Biswanath Saha

    2018-03-01

    Full Text Available The management of terrestrial weed is of great concern for the scientific community as these weeds cause adverse effect in different ecosystems like forest, agriculture and urban. The widespread of these weeds by their adaptive capability and morphological advancement is difficult to control. Parthenium hysterophorous, Lantana camara, Saccharum spontaneum, Ageratum conyzoides are the weeds that spread all over the world. There are various management practices employed for the control of this weeds. But all of these practices have some drawbacks those are neither environment friendly nor economical. In this paper a review has been done to evaluate various alternative management practices for these terrestrial weeds and to analyze their feasibility. Vermicomposting and anaerobic digestion can be viable alternative option which is cost effective as well. There are few studies regarding vermicomposting and anaerobic digestions of terrestrial weeds are done.

  11. RUST INCIDENCE AND SEVERITY IN SUGARCANE CLONES IN THE PARANÁ STATE, BRAZIL INCIDÊNCIA E SEVERIDADE DA FERRUGEM EM CLONES DE CANA-DE-AÇÚCAR NO ESTADO DO PARANÁ

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    Oswaldo Teruyo Ido

    2007-09-01

    Full Text Available

    Promising clones in the third selection phase of the sugarcane genetic breeding program in Paraná State, Brazil, were evaluated for the severity of sugarcane rust (Puccinia melanocephala incidence under natural infection. The evaluation was carried out in a sugarcane plant and a sugarcane ratoon cycles, from 1998 to 2000. The assessment of severity identified fifteen clones with rust resistance reaction in the sugarcane plant cycle. Of the 41 evaluated clones, 18.2% presented slightly resistant to susceptible reaction. The highest rust incidence was observed in the early phases of sugarcane crop development, from three to six months. Among the clones with resistance reaction to rust, five were derived from the same female parent (RB855589. This fact demonstrates the good potential of this genotype for crosses looking at clones with rust-resistance reaction.

    KEY-WORDS: Saccharum spp.; Puccinia melanocephala; resistance; selection.

    Clones promissores do Programa de Melhoramento Genético da Cana-de-Açúcar no Estado do Paraná em terceira fase de seleção foram avaliados quanto à incidência e severidade da ferrugem da cana-de-açúcar (Puccinia melanocephala sob condições de infecção natural. A avaliação foi realizada nos ciclos de cana-planta e cana-soca, entre os anos 1998 e 2000. Os resultados de severidade permitiram agrupar no ciclo de cana-planta, quinze clones com reação resistente a ferrugem. Dos 41 clones avaliados, 18,2% apresentaram reação moderadamente resistente a suscetível. Observou-se a maior incidência da ferrugem nas primeiras fases de desenvolvimento da cana-de-açúcar, entre os três a seis meses. Entre os clones que apresentaram reação resistente, verificou-se que cinco foram oriundos do mesmo genitor feminino (RB855589. Isso demonstra o bom potencial

  12. Diversidade de Gluconacetobacter diazotrophicus isolada de plantas de cana-de-açúcar cultivadas no Brasil Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil

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    Liamara Perin

    2004-08-01

    Full Text Available O objetivo deste trabalho foi avaliar a diversidade da população de Gluconacetobacter diazotrophicus oriunda de cultivares de cana-de-açúcar (Saccharum spp. de diferentes regiões e bancos de germoplasma. O estudo foi realizado com 123 isolados, obtidos de folhas, colmos e raízes de 80 espécies e híbridos de cana-de-açúcar, procedentes de diferentes países e mantidos em coleções de germoplasma nos Estados da Bahia e Rio de Janeiro. Foram utilizados cinco isolados obtidos de plantas de café (Coffea arabica, dois de abacaxi (Ananas comosus e um de Pennisetum purpureum e mais 10 estirpes com padrões eletroforéticos distintos, após o uso de enzimas comuns do metabolismo microbiano (MLEE. O agrupamento obtido por meio da técnica de imunoadsorção com enzima acoplada (ELISA sugere que as variações expressas pelos isolados não estão relacionadas com a espécie de planta, a variedade de cana-de-açúcar, a origem geográfica, a parte da planta de onde os isolados foram obtidos e o tempo de amostragem. Altas doses de nitrogênio levaram à diminuição da diversidade de G. diazotrophicus.The objective of this study was to evaluate the diversity of Gluconacetobacter diazotrophicus isolates from different sugarcane (Saccharum spp. varieties. This study was developed using 123 strains from the internal tissues of stems and roots, isolated from 80 species and hybrids of sugarcane, originated from different countries and maintained in two germoplasm collections localized in Bahia and Rio de Janeiro States. Five isolates obtained from coffee plants (Coffea arabica, two from pineapple (Ananas comosus, one from Pennisetum purpureum and 10 strains, which present different electrophoretic patterns, were used in the comparison. Cluster analysis of enzyme-linked immunosorbent assay (ELISA results suggested that variations were not correlated with the plant species, sugarcane variety, geographic origin, parts of plants nor with sampling time

  13. Evaluación de indicadores productivos en conejas mestizas con una dieta basada en forraje y pienso criollo Evaluation of productive indicators in crossbred does with a diet based on forage and homemade concentrate

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    O López

    2011-03-01

    Full Text Available Con el objetivo de evaluar el comportamiento productivo de conejas mestizas se utilizaron un total de 24 reproductoras, entre 10 y 18 meses de edad, con un peso promedio superior a los 3,0 kg durante cuatro meses. Las reproductoras consumieron una dieta constituida por: forraje de morera (Morus alba: 0,30 kg; caña (Saccharum officinarum molida: 0,25 kg, glycine (Neonotonia wightii: 0,40 kg y pienso criollo: 0,06 kg. Las crías se pesaron al nacimiento, a los 20 y a los 45 días de edad. En las reproductoras se controló las crías nacidas vivas y destetadas por parto. Se obtuvo como promedio 6,4 gazapos vivos por parto con 0,054 kg de PV al nacimiento y se destetaron 5,4 crías a los 45 días de edad con un peso de 0,694 kg. Además, se encontró un 84,4% de supervivencia durante la etapa de lactación. La ganancia media diaria durante la lactancia fue de 0,014 kg/animal/día. Los resultados evidenciaron que con la dieta propuesta se obtuvieron indicadores productivos alentadores en reproductoras mestizas.With the objective of evaluating the productive performance of crossbred does, a total of 24 animals were used, between 10 and 18 months old, with average weight higher than 3,0 kg, during four months. The does ate a diet constituted by mulberry (Morus alba forage: 0,30 kg; ground sugarcane (Saccharum officinarum: 0,25 kg; glycine (Neonotonia wightii: 0,40 kg and homemade concentrate: 0,06 kg. The young rabbits were weighed at birth, 20 and 45 days after birth. In the does the offspring born alive and weaned per parturition, were controlled. As average, 6,4 live rabbits were obtained per parturition, with 0,054 kg LW at birth and 5,4 rabbits were weaned being 45 days old and weighing 0,694 kg. In addition, 84,4% survival was found during the lactation stage. The mean daily gain during lactation was 0,014 kg/animal/day. The results proved that with the proposed diet encouraging productive indicators were obtained in crossbred does.

  14. Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells

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    Maria Izabel Gallão

    2010-04-01

    Full Text Available Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.Células de Saccharum officinarum quando submetidas a quitina hidrolisada por 1 a 8h produziram material fenólico. Essas alterações foram observadas por meio de métodos citoquímicos como o Azul de Toluidina e Floroglucinol/HCl. Após 4 h, além das mudanças nas paredes celulares houve uma mudança no padrão nuclear das células tratadas com quitina. Por observação da reação de Feulgen, houve um aumento de 96% na área nuclear no material em 6h. Para as células tratadas foram observadas regiões de cromatina compactada e um processo de degeneração do nucléolo. Nas áreas externas da parede celular existia uma desagregação dos polisacarídios confirmando os resultados obtidos para diferentes plantas com o uso de outros elicitores. A atividade da peroxidase foi maxima após 4 h e então decresceu progressivamente. A atividade da PAL aumentou a partir de 4 h de incubação. Estes resultados mostram que o hidrolisado de quitina estimula as respostas de defesa em células de cana.

  15. Evaluation of mathematical methods for predicting optimum dose of gamma radiation in sugarcane (Saccharum sp.)

    International Nuclear Information System (INIS)

    Wu, K.K.; Siddiqui, S.H.; Heinz, D.J.; Ladd, S.L.

    1978-01-01

    Two mathematical methods - the reversed logarithmic method and the regression method - were used to compare the predicted and the observed optimum gamma radiation dose (OD 50 ) in vegetative propagules of sugarcane. The reversed logarithmic method, usually used in sexually propagated crops, showed the largest difference between the predicted and observed optimum dose. The regression method resulted in a better prediction of the observed values and is suggested as a better method for the prediction of optimum dose for vegetatively propagated crops. (author)

  16. Yield emulsifiers exopolysaccharides produced by native halophilic bacteria concentrations molasses three Saccharum officinarum L. "sugarcane"

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    Ángel Fuentes, Carmen Carreño

    2013-06-01

    Full Text Available The microbial exopolysaccharide with emulsifying properties are an alternative to polymers and chemicals from algae and plants. Its production in molasses as carbon source lowers costs and generates added value to this byproduct of the sugar industry, so the aim of this study was to determine the performance and productivity of EPS emulsifiers by native halophilic bacteria in 20, 30 and 40 gL-1 of molasses. In MY synthetic medium with 5 % w/v of salts, 138 isolates of bacteria obtained from soil samples of salt water and in the districts of San Jose and Santa Rosa, in Lambayeque. In 10.8 % of these gummy colony forming bacteria and grown on glucose as carbon source EPS recovered whose maximum values of the mixtures in water emulsion - oil phase were 63.3 and 56.6 % after 1 and 24 hours, respectively. The M5 bacteria identified as Halomonas C1 10-1 sp. M5 EPS synthesized emulsifiers molasses broth, reaching yields Yp/s of 0.296 gg-1 and 0.200 gg-1 with 20 and 30 gL-1 of molasses respectively, a productivity of 0.016 and 0.017 gL-1 h -1 , not differing significantly between them. With 10 gL-1 glucose was reached Yp/s of 0.171 gg-1 and a productivity of 0.018 gL-1 h -1 . It was shown that the EPS produced native halophilic bacteria utilizing molasses emulsifiers as carbon source.

  17. In vitro effects of policosanol (Saccharum officinarum L wax alcohols on the 5-lipooxygenase enzyme

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    Yohani Pérez Guerra

    Full Text Available Introduction: policosanol, a mixture of high molecular weight aliphatic alcohols purified from sugarcane with octacosanol as the main component, shows cholesterol-lowering and antiplatelet effects in addition to an inhibitory effect on type I cicloxygenase. Objective: to determine whether policosanol may inhibit 5-LOX enzyme activity in vitro. Methods: effects on 5-LOX enzyme activities were assessed in rat blood polymorphonuclear leukocytes. Vehicle or Policosanol suspensions (0.6 to 6 000 µg/mL were added to tubes containing the reaction mix and then absorbance changes at 234 nm were measured. Results: added Policosanol inhibited in vitro 5-LOX activity by 30 %, which was not a significant figure but depended on the concentration(r= 0.992; p< 0.05; it was 1 250 µg/mL. Conclusions: policosanol did not significantly inhibit 5-LOX enzyme activity in rat PMNL preparations, so that it does not seem to be a dual inhibitor of COX and-LOX enzymes. This result differs from that found for beeswax alcohols and underlines the different effects of the mixtures of long-chain fatty alcohols purified from the sugarcane and the beeswax.

  18. Candida middelhoveniana sp. nov., a new yeast species found on the rhizoplane of organically cultivated sugarcane.

    Science.gov (United States)

    Ribeiro, José R de A; Carvalho, Patrícia M B de; Cabral, Anderson de S; Macrae, Andrew; Mendonça-Hagler, Leda C S; Berbara, Ricardo L L; Hagler, Allen N

    2011-10-01

    A novel yeast species within the Metschnikowiaceae is described based on a strain from the sugarcane (Saccharum sp.) rhizoplane of an organically managed farm in Rio de Janeiro, Brazil. The D1/D2 domain of the large subunit ribosomal RNA gene sequence analysis showed that the closest related species were Candida tsuchiyae with 86.2% and Candida thailandica with 86.7% of sequence identity. All three are anamorphs in the Clavispora opuntiae clade. The name Candida middelhoveniana sp. nov. is proposed to accommodate this highly divergent organism with the type strain Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (IMUFRJ) 51965(T) (=Centraalbureau voor Schimmelcultures (CBS) 12306(T), Universidade Federal de Minas Gerais (UFMG)-70(T), DBVPG 8031(T)) and the GenBank/EMBL/DDBJ accession number for the D1/D2 domain LSU rDNA sequence is FN428871. The Mycobank deposit number is MB 519801.

  19. Sugarcane maturity estimation through edaphic-climatic parameters

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    Scarpari Maximiliano Salles

    2004-01-01

    Full Text Available Sugarcane (Saccharum officinarum L. grows under different weather conditions directly affecting crop maturation. Raw material quality predicting models are important tools in sugarcane crop management; the goal of these models is to provide productivity estimates during harvesting, increasing the efficiency of strategical and administrative decisions. The objective of this work was developing a model to predict Total Recoverable Sugars (TRS during harvesting, using data related to production factors such as soil water storage and negative degree-days. The database of a sugar mill for the crop seasons 1999/2000, 2000/2001 and 2001/2002 was analyzed, and statistical models were tested to estimate raw material. The maturity model for a one-year old sugarcane proved to be significant, with a coefficient of determination (R² of 0.7049*. No differences were detected between measured and estimated data in the simulation (P < 0.05.

  20. Utilization of emergent aquatic plants for biomass-energy-systems development

    Energy Technology Data Exchange (ETDEWEB)

    Kresovich, S.; Wagner, C.K.; Scantland, D.A.; Groet, S.S.; Lawhon, W.T.

    1982-02-01

    A review was conducted of the available literature pertaining to the following aspects of emergent aquatic biomass: identification of prospective emergent plant species for management; evaluation of prospects for genetic manipulation; evaluation of biological and environmental tolerances; examination of current production technologies; determination of availability of seeds and/or other propagules, and projections for probable end-uses and products. Species identified as potential candidates for production in biomass systems include Arundo donax, Cyperus papyrus, Phragmites communis, Saccharum spontaneum, Spartina alterniflora, and Typha latifolia. If these species are to be viable candidates in biomass systems, a number of research areas must be further investigated. Points such as development of baseline yield data for managed systems, harvesting conceptualization, genetic (crop) improvement, and identification of secondary plant products require refinement. However, the potential pay-off for developing emergent aquatic systems will be significant if development is successful.

  1. Host Plants of Xylosandrus mutilatus in Mississippi

    International Nuclear Information System (INIS)

    Stone, W.D.; Nebeker, T.E.; Gerard, P.D.

    2007-01-01

    Host range of Xylosandrus mutilatus (Blandford) in North America is reported here for the first time. Descriptive data such as number of attacks per host, size of stems at point of attacks, and height of attacks above ground are presented. Hosts observed in Mississippi were Acer rubrum L., Acer saccharum Marsh., Acer palmatum Thunb., Ostrya virginiana (Mill.) K. Koch., Cornus florida L., Fagus grandifolia Ehrh., Liquidamber styraciflua L., Carya spp., Liriodendron tulipifera L., Melia azedarach L., Pinus taeda L., Prunus serotina Ehrh., Prunus americana Marsh., Ulmus alata Michaux, and Vitus rotundifolia Michaux. Liquidamber styraciflua had significantly more successful attacks, significantly higher probability of attacks, and significantly higher number of adult beetles per host tree than did Carya spp., A. rubrum, and L. tulipifera. This information is relevant in determining the impact this exotic beetle may have in nurseries, urban areas, and other forestry systems where this beetle becomes established. (author) [es

  2. Bayesian analysis for uncertainty estimation of a canopy transpiration model

    Science.gov (United States)

    Samanta, S.; Mackay, D. S.; Clayton, M. K.; Kruger, E. L.; Ewers, B. E.

    2007-04-01

    A Bayesian approach was used to fit a conceptual transpiration model to half-hourly transpiration rates for a sugar maple (Acer saccharum) stand collected over a 5-month period and probabilistically estimate its parameter and prediction uncertainties. The model used the Penman-Monteith equation with the Jarvis model for canopy conductance. This deterministic model was extended by adding a normally distributed error term. This extension enabled using Markov chain Monte Carlo simulations to sample the posterior parameter distributions. The residuals revealed approximate conformance to the assumption of normally distributed errors. However, minor systematic structures in the residuals at fine timescales suggested model changes that would potentially improve the modeling of transpiration. Results also indicated considerable uncertainties in the parameter and transpiration estimates. This simple methodology of uncertainty analysis would facilitate the deductive step during the development cycle of deterministic conceptual models by accounting for these uncertainties while drawing inferences from data.

  3. Nitrogen Deposition Reduces Decomposition Rates Through Shifts in Microbial Community Composition and Function

    Science.gov (United States)

    Waldrop, M.; Zak, D.; Sinsabaugh, R.

    2002-12-01

    Atmospheric nitrogen (N) deposition may alter soil biological activity in northern hardwood forests by repressing phenol oxidase enzyme activity and altering microbial community composition, thereby slowing decomposition and increasing the export of phenolic compounds. We tested this hypothesis by adding 13C-labelled cellobiose, vanillin, and catechol to control and N fertilized soils (30 and 80 kg ha-1) collected from three forests; two dominated by Acer Saccharum and one dominated by Quercus Alba and Quercus Velutina. While N deposition increased total microbial respiration, it decreased soil oxidative enzyme activities, resulting in slower degradation rates of all compounds, and larger DOC pools. This effect was larger in the oak forest, where fungi dominate C-cycling processes. DNA and 13C-phospolipid analyses showed that N addition altered the fungal community and reduced the activity of fungal and bacterial populations in soil, potentially explaining reduced soil enzyme activities and incomplete decomposition.

  4. Efeitos da defaunação em ovinos alimentados com cana-de-açúcar (Saccharum officinarum, L. adicionada de uréia Effects of defaunation in sheep fed sugar cane (Saccharum officinarum, L. plus urea

    Directory of Open Access Journals (Sweden)

    F.C.F. Lopes

    2002-04-01

    Full Text Available Em delineamento inteiramente ao acaso, estudou-se o efeito da defaunação em ovinos que receberam cana-de-açúcar e uréia sobre o consumo e digestibilidade aparente de nutrientes, dinâmica da fase sólida, parâmetros de fermentação ruminal e de degradação in situ. As taxas de passagem no rúmen foram, respectivamente, de 3,05 e 1,97%/h, para ovinos faunados e defaunados. Valores mais elevados para taxa (5,4%/h e extensão (46,7% da digestão ruminal e de degradabilidade efetiva da FDN da cana (31,5% foram obtidos nos ovinos faunados. Seus consumos diários (57,7 e 32,3 g/kg0,75, respectivamente para MS e FDN foram superiores (PThe defaunation effect on feed intake, nutrient apparent digestibilities, solid phase dynamics, ruminal fermentation parameters and in situ degradation in sheep fed on sugar cane plus urea was studied in a completely randomized design with three replicates. Ruminal passage rates of 3.05 and 1.97%/h were observed for the faunated and defaunated groups, respectively. Higher values for sugar cane NDF ruminal digestion rate (5.41%/h, extension (46.7% and effective degradability (31.53% were observed for the faunated group. The faunated group daily intake (57.7 and 32.3 g/kg0.75 for dry matter and NDF residue, respectively was higher (P<0.06 than that for the defaunated group. Total VFA, acetate and propionate concentrations were higher (P<0.05 in the faunated group and were dependent on sampling time. Defaunated sheep showed higher (P<0.04 post prandial decrease in pH. Relative to apparent digestibility difference (P<0.05 between treatments was observed only for dry matter.

  5. Contribution of bacterial cell nitrogen to soil humic fractions

    International Nuclear Information System (INIS)

    Knowles, R.; Barro, L.

    1981-01-01

    Living cells of Serratia marcescens, uniformly labelled with 15 N, were added to samples of maple (Acer saccharum) and black spruce (Picea mariana) forest soils. After different periods of incubation from zero time to 100 days, the soils were subjected to alkali-acid and phenol extraction to provide humic acid, fulvic acid, humin and 'humoprotein' fractions. Significant amounts of the cell nitrogen were recovered in the humic and fulvic acids immediately after addition. After incubation, less cell nitrogen appeared in the humic acid and more in the fulvic acid. The amount of cell nitrogen recovered in the humin fraction increased with incubation. Roughly 5 to 10 per cent of the added cell nitrogen was found as amino acid nitrogen from humoprotein in a phenol extract of the humic acid. The data are consistent with the occurrence of co-precipitation of biologically labile biomass nitrogen compounds with humic polymers during the alkaline extraction procedure involved in the humic-fulvic fractionation. (orig.)

  6. Scale insects (Hemiptera: Coccomorpha) on sugarcane in Colombia, with description of a new species of Tillancoccus Ben-Dov (Coccidae).

    Science.gov (United States)

    Caballero, Alejandro; Ramos-Portilla, Andrea Amalia; Kondo, Takumasa

    2017-05-02

    Herein we describe a new species, Tillancoccus koreguajae Caballero & Ramos, sp. n. (Hemiptera: Coccidae) from Colombia collected on sugarcane. Pinnaspis strachani (Cooley) is also recorded on sugarcane for the first time worldwide. An updated list of seven species of Coccomorpha on sugarcane in Colombia is provided, including information on its distribution, biology, and mutualistic ants for each species. Seven species of scale insects (Hemiptera: Coccomorpha) have been recorded previously on sugarcane, Saccharum officinarum L. (Poaceae) in Colombia: Pseudococcidae: Dysmicoccus boninsis (Kuwana), D. brevipes (Cockerell), Pseudococcus calceolariae (Maskell), Saccharicoccus sacchari (Cockerell); Coccidae: Pulvinaria elongata Newstead; Diaspididae: Duplachionaspis divergens (Green) and Serenaspis minima (Maskell). However, the record of S. minima in Colombia is considered doubtful as there are no voucher specimens from Colombia and because the distribution of this species is currently limited to the Australasian region. Pseudococcus calceolariae is present in Colombia but its record on sugarcane is also doubtful. A taxonomic key for the identification of scale insects on sugarcane in Colombia is provided.

  7. Effect of enhanced UV-B radiation of adaxial leaf surface micromorphology and epicuticular wax biosynthesis of sugar maple

    International Nuclear Information System (INIS)

    Gordon, D.C.; Percy, K.E.; Riding, R.T.

    1998-01-01

    Sugar maple (Acer saccharum [Marsh.]) seedlings were exposed to UV-B BE ranging from 0.61 kJ m -2 d -1 to 12.48 kJ m -2 d -1 . Increasing UV-B intensity was associated with changes in micromorphological characteristics of the adaxial leaf surface. In vivo incorporation of [1- 14 C] acetate into sugar maple adaxial leaf surface epicuticular wax indicated (p<0.05) a UV-B sensitivity threshold at or near 6.2 kJ m -2 d -1 . Exposure to dosages greater than 6.2 kJ m -2 d -1 resulted in a significant (p<0.05) decrease in wax biosynthesis. The proportion of [1- 14 C] acetate incorporated into each of the different epicuticular wax classes changed with increasing UV-B. Incorporation of [1- 14 C] acetate into alkyl esters decreased while incorporation into alkanes increased with increasing UV-B dose. The effects of enhanced UV-B dose recorded in this experiment may have implications for cuticle function. (author)

  8. AMMI analysis to evaluate the adaptability and phenotypic stability of sugarcane genotypes

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    Luís Cláudio Inácio da Silveira

    2013-02-01

    Full Text Available Sugarcane (Saccharum sp. is one of the most important crops in Brazil. The high demand for sugarcane-derived products has stimulated the expansion of sugarcane cultivation in recent years, exploring different environments. The adaptability and the phenotypic stability of sugarcane genotypes in the Minas Gerais state, Brazil, were evaluated based on the additive main effects and multiplicative interaction (AMMI method. We evaluated 15 genotypes (13 clones and two checks: RB867515 and RB72454 in nine environments. The average of two cuttings for the variable tons of pol per hectare (TPH measure was used to discriminate genotypes. Besides the check RB867515 (20.44 t ha-1, the genotype RB987935 showed a high average TPH (20.71 t ha-1, general adaptability and phenotypic stability, and should be suitable for cultivation in the target region. The AMMI method allowed for easy visual identification of superior genotypes for each set of environments.

  9. In vitro degradability and total gas production of biodiesel chain byproducts used as a replacement for cane sugar feed

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    Milenna Nunes Moreira

    2014-09-01

    Full Text Available This study aimed to determine the in vitro degradability of dry matter and the total gas production of oil seed press cake from biodiesel production (Gossypium hirsutum L., Helianthus annuus L., Ricinus communis, Moringa oleífera L. and Pinhão manso curcas L. at four different levels of replacement (0, 30, 50, and 70% for cane sugar (Saccharum officinarum RB. in ruminant feed. Inocula were prepared using the ruminal fluid of three Holstein cows, and data were collected after 48 hours of incubation. The byproducts of Moringa had the highest degradability, and castor presented the lowest values at all evaluated levels of replacement. Castor bean byproduct showed the highest total gas production, cotton showed the lowest production, and the byproduct of Moringa at the 70% level showed the best ruminal fermentation results. These results demonstrate that the use of oil seed press cake from biodiesel production (Helianthus annuus L. and Ricinus communis can replace cane sugar in ruminant feed.

  10. Mutation breeding in sugar-cane (Saccharum sp. hybrid) by gamma irradiation of cuttings and tissue cultures

    International Nuclear Information System (INIS)

    Siddiqui, S.H.; Javed, M.

    1982-01-01

    The sugar-cane variety Co 547, which is highly susceptible to smut disease (Ustilago scitaminea Syd.) and is also late maturing, was exposed to different doses of gamma radiation to study its radiosensitivity and to induce smut disease resistance and early maturity. The radiosensitivity of the variety showed that an optimum dose (LD 50 ) was 2.0 kR and the working dose range was found to be 1.5-3.0 kR, whereas doses higher than 4.0 kR drastically affected the growth and germination. A broad spectrum of variability in reaction to disease resistance was observed after radiation exposure. This facilitated the isolation of disease-resistant mutants. Twenty-three mutants showing varied reaction to smut under field infection conditions were tested for two years by artificial inoculation using the dip method. From these studies 15 stable mutants were isolated. Of 15 mutants, seven showed promising performance in cane yield and sucrose contents. The tissue culture technique was used to determine the potential of different commercial clones for callusing. In test explants callusing was achieved readily and proliferation of callus was fairly good in all the clones. (author)

  11. Obtainment of sugarcane (Saccharum spp. hybrid biotechnological seed of high genetic and phytosanitary quality in Ecuador

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    Miguel M. Ramos-Leal

    2015-01-01

    Full Text Available Methods for obtaining healthy plants are mainly based on the combined use of meristem in vitro culture and hydrothermotherapy. This work was carried out in order to obtain free- pathogen plants for using as seeds, emphasizing ratoon stunting disease (RSD of sugarcane. The work was performed using a group of sugarcane cultivars, which was confirmed the variety identification by means of peroxidases isozymes electrophoretic patterns. Plant sanitation was performed in three successive steps by means of hydrothermal treatment at 52 oC during 2h and using the fungicide vitavax, a second hydrothermal treatment at 51 °C during 10 min to the meristem and a third step in which vitroplantlets were propagated in presence of the antibiotic gentamicin. RSD detection was done by using the staining transpiration methods (STM of functional bundles. Results allowed the creation of a germoplasm bank of sugarcane plants free of the most important systemic bacterial pathogens, with high genetic quality, which provided basic seed of excellent quality for establishing seedlings. Conservation included an in vitro-plantlets bank and the creation of a "DNA bank" which allows preserving the genetic fond in laboratory conditions. Finally a methodology for obtaining free-pathogens plants from buds and meristems is included.

  12. Altered growth and fine root chemistry of Betula papyrifera and Acer saccharum under elevated CO2

    International Nuclear Information System (INIS)

    Parsons, W. F. J.; Kopper, B. J.; Lindroth, R. L.

    2003-01-01

    The effects of carbon dioxide enrichment on fine root chemical composition of two tree species common to northern hardwood forests was investigated. Results showed no change in the root/shoot ratios and fine root percentages in either birch or maple samples as a result of enriched carbon dioxide. Tissue nitrogen concentrations decreased in the fine roots, and consequently, carbon/nitrogen ratios increased with elevated carbon dioxide. In birch only, condensed tannins increased with carbon dioxide enrichment; in maple, neither complex tannins nor hydrosable tannins appear to have been influenced by elevated carbon dioxide. It is suspected that the responses of the tree saplings to elevated carbon dioxide may be related to their successional status. 37 refs., 2 tabs

  13. Área superficial de carbones activados y modificados obtenidos del recurso agrícola Saccharum officinarum

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    Fredy Colpas C.

    2017-12-01

    Full Text Available El carbón activado es un material utilizado industrialmente en adsorción de gases, filtrados, limpieza de líquidos y como soporte para catalizadores en medios no oxidantes. Además tiene varias aplicaciones ambientales como en la adsorción de iones metálicos de plomo. El objetivo de este trabajo fue la obtención de carbones activados carbonizando biomasa de caña de azúcar a 400°C en atmósfera de nitrógeno, activando con ácido fosfórico y oxidando con ácido nítrico o peróxido de hidrógeno seguido de tratamiento térmico. La preparación de carbones activados a partir de residuos agrícolas está desarrollándose con excelentes resultados potencialmente aplicables. Bajo este contexto, se muestra un método de preparación mediante activación química con ácido fosfórico, la cual aumentó el contenido de carbono y disminuyó el de oxígeno, seguidamente con el objeto de estudiar el desarrollo del área superficial se somete a proceso de oxidación con calentamiento térmico. Los carbones obtenidos fueron caracterizados mediante análisis proximal y para la determinación de los grupos funcionales oxigenados se utilizó la espectroscopia infrarroja de reflectancia difusa-transformada de Fourier. La determinación de grupos oxigenados básicos y ácidos se realizó por el método Boehm. Para la medición del área superficial se utilizó el método BET. El área de microporos se incrementó por el tratamiento con HNO3 y H2O2 desde 278 m2/g hasta 402 y 446m2/g, respectivamente. La mayor parte del área en los carbones se debió a los microporos. El pH de los carbones fue aproximadamente de tres y los grupos COOH alcanzaron valores hasta 2,12meq/g.

  14. Eficiência no uso da água na cana-de-açúcar sob diferentes lâminas de irrigação e níveis de zinco no litoral paraibano Water use efficiency in sugarcane crop under different depths of irrigation and zinc doses in coastal region of Paraíba, Brazil

    Directory of Open Access Journals (Sweden)

    Carlos H. A. Farias

    2008-09-01

    Full Text Available Esta pesquisa foi conduzida em área experimental da Fazenda Capim II, localizada no Município de Capim - PB, com o intuito de determinar a eficiência no uso da água (EUA pela cana-de-açúcar (Saccharum officinarum L., cultivar SP 79-1011. O delineamento experimental consistiu em blocos casualizados, com três repetições, em esquema fatorial 5 x 5. Os tratamentos referentes à lâmina de irrigação foram: sequeiro; 25; 50; 75 e 100% da ETc (1.026,57 mm com as respectivas precipitações efetivas de 780,06; 713,31; 487,54; 243,71 e 194,51 mm; os níveis de zinco estudados foram 0; 1; 2; 3 e 4 kg ha-1. O equipamento de irrigação utilizado foi do tipo pivô central, com turno de rega de nove dias. A eficiência no uso da água (EUA na produção de colmos e de açúcar aumenta à medida que se eleva a lâmina total de água aplicada à cultura. Para a região norte-paraibana dos tabuleiros costeiros, a EUA na produção de colmo e de açúcar, de forma maximizada, é 7,12 e 0,67 kg m-3, respectivamente.The study was conducted in an experimental area of the Capim II Farm, located in the municipality of Capim - PB, Brazil, with the objective of determining the efficiency in the use of the water (WUE for the sugarcane crop (Saccharum officinarum L., cultivar SP 79-1011. The experimental design consisted of randomized blocks, with three replications in a 5 x 5 factorial design. The irrigation treatments were: rainfed; 25; 50; 75 and 100% of the ETc (1,026.57 mm with the respective effective precipitations of 780.06; 713.31; 487.54; 243.71 and 194.51 mm. The levels of zinc studied were 0; 1; 2; 3 and 4 kg ha-1. The irrigation equipment used in the study was a central pivot with irrigation frequency of nine days. The water use efficiency (WUE of sugarcane crop in terms of cane production and of sugar increased with the depth of water applied. For the Coastal Table Lands of northern Paraiba, the maximized WUE for sugarcane and sugar production is

  15. ESTIMULACIÓN CON CAMPO ELECTROMAGNÉTICO VARIABLE DE MICROORGANISMOS BENÉFICOS APLICADOS A LA CACHAZA PARA MEJORAR SU USO COMO BIOFERTILIZANTE ESTIMULAÇÃO COM VARIÁVEL DO CAMPO ELETROMAGNÉTICO BENÉFICOS UTILIZADOS MICRORGANISMOS PARA MELHORAR O CACHAÇA USE COMO BIOFERTILIZANTES ESTIMULATION WITH A VARIABLE ELECTROMAGNETIC FIELD OF BENEFICIAL MICROORGANISMS APPLIED TO SUGARCANE BAGASSE TO ENHANCE THEIR USE AS BIOFERTILIZER

    Directory of Open Access Journals (Sweden)

    ORLANDO ZÚÑIGA E

    2011-12-01

    Full Text Available Con el objetivo de acelerar la acción dinámica de los microorganismos benéficos estimulándolos electromagnéticamente para descomponer las moléculas complejas del material orgánico de la cachaza (subproducto de la caña de azúcar Saccharum officinarum L. a sustancias más simples, facilitando la absorción de elementos hacia la planta, se evaluó la eficiencia productiva y el grado de descomposición orgánica de los siguientes tratamientos: cachaza fresca sin microorganismos (F, cachaza fresca con microorganismos no estimulados electromagnéticamente (FM, cachaza fresca con microorganismos estimulados electromagnéticamente (FME y cachaza madura sin microorganismos (C, se optimizó la configuración del equipo de estimulación electromagnética obteniéndose como mejor tratamiento el (FME con 1 hora en tiempo de exposición de los microorganismos al campo electromagnético, de 250 Hz en frecuencia, y 15 mT de magnitud del campo electromagnético, el cual aceleró el proceso natural de descomposición orgánica en un 49,1% de la cachaza fresca al aproximarla al grado de mineralización de la cachaza madura, lo cual mostró la efectividad de la técnica electromagnética.A fim de acelerar a ação dinâmica de estimulação eletromagnética microrganismos benéficos para quebrar moléculas complexas de matéria orgânica da torta (subproduto da cana-de-açúcar Saccharum officinarum L. a substâncias mais simples, facilitando a absorção da planta para, avaliou a eficiência da produção e do grau de decomposição orgânica dos seguintes tratamentos: rum fresco sem microrganismos (F, o rum fresco eletromagneticamente estimulados com microrganismos (FM, rum fresco com microrganismos estimulada electromagnéticos (FME e maduros sem microrganismos rum (C foi otimizado configurações equipamentos de estimulação eletromagnética melhor tratamento obtido como (FME, com 1 hora de tempo de exposição dos microrganismos ao campo eletromagn

  16. Use of SSR markers for DNA fingerprinting and diversity analysis of Pakistani sugarcane (Saccharum spp. hybrids) cultivars

    Science.gov (United States)

    In recent years SSR markers have been used widely for genetic analysis. The objective of this study was to use an SSR-based marker system to develop the molecular fingerprints and analyze the genetic relationship of sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers wer...

  17. Comparative study on agro-physiology of sugarcane (saccharum officinarum l.) genotypes at different irrigation co-efficient values

    International Nuclear Information System (INIS)

    Farooq, Z.; Mehmood, S.

    2015-01-01

    Drought is the primary factor limiting sugarcane growth and physiological development under the climatic conditions of Pakistan; especially in those areas where without supplemental irrigation, productivity is not possible. Lack of detailed information regarding the performance of cane varieties under drought during formative stage and poor selection breeding program played key role in limiting cane productivity. The proposed study was conducted to investigate the genetic response of different cultivars viz., CSSG-676, CSSG-668, HoSG-795, HoSG-529, NSG-59 and HSF- 240 (standard) regarding the physiological development of sugarcane and its productivity at different irrigation co-efficient levels (100%, 80% and 60%). This study elucidates that moisture has a pronounced impact on the physiological attributes of sugarcane and proper irrigation scheduling with 20 no. of irrigations were reported best in-term of better germination (69.65%), leaf area index (7.13), crop growth rate (8.44), net assimilation rate (1.06) and chlorophyll contents (5.98). Similarly in case of genomic response, NSG-59 was reported significant best as compared to all other test cultivars in term of better physiological performance, showing significant higher leaf area index, crop growth rate, chlorophyll contents and water use efficiency that maximized the crop growth and resulted in higher net assimilation rate. Higher proline contents (1.59) produced in NSG-59 also made it best under drought conditions. (author)

  18. Binding of nickel and zinc ions with activated carbon prepared from sugar cane fibre (Saccharum officinarum L.

    Directory of Open Access Journals (Sweden)

    E.U. Ikhuoria

    2007-04-01

    Full Text Available Activated carbon was prepared from sugar cane fibre by carbonizing at 500 oC for 30 minutes. This was followed by activation with ammonium chloride. The activated carbon was characterised in terms of pH, bulk density, ash content, surface area and surface charge. Equilibrium sorption of nickel and zinc ions by the activated carbon was studied using a range of metal ion concentrations. The sorption data was observed to have an adequate fit for the Langmuir isotherm equation. The level of metal ion uptake was found to be of the order: Ni2+ > Zn2+. The difference in the removal efficiency could be explained in terms of the hydration energy of the metal ions. The distribution coefficient for a range of concentration of the metal ions at the sorbent water interface is found to be higher than the concentration in the continuous phase.

  19. Extraction optimization by response surface methodology: Purification and characterization of phytosterol from sugarcane (Saccharum officinarum L.) rind.

    Science.gov (United States)

    Feng, Simin; Luo, Zisheng; Zhong, Zhou; Jiang, Lei; Tang, Kaichen

    2014-06-01

    A green, simple, and effective method for the extraction of sugarcane lipids from sugarcane rind was investigated by response surface methodology. The optimum conditions of technological progress obtained through response surface methodology were as follows: liquid-to-solid ratio 7.94: 1 mL/g, extraction temperature 50°C and extraction time 5.98 h. The practical sugarcane lipids extraction yield was 6.55 ± 0.28%, which was in good consistence with the predicted extraction yield of 6.47%. The results showed that the sugarcane lipids extraction yield obtained in optimum conditions increased by 1.16∼7.28-fold compared to the yields obtained in single-factor experiments. After saponification and SPE steps, the nonsaponifiable fraction of sugarcane lipids was analyzed by gas chromatography with mass spectrometry and high-performance liquid chromatography. β-Sitosterol, stigmasterol, and campesterol were the prevailing phytosterols in the sample, while fucosterol, gramisterol, stigmast-7-en-3-ol, (3β,5α,24S)-, stigmasta-4,6,22-trien-3α-ol, and cholest-8(14)-en-3β-ol acetate were also identified as minor steroids. Furthermore, the content of β-sitosterol and a mixture of campesterol and stigmasterol (quantified by high-performance liquid chromatography) was 44.18 mg/100 g dry weight and 43.20 mg stigmasterol/100 g dry weight, respectively. Our results indicate that sugarcane rind is a good source of phytosterol. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. In situ enzyme aided adsorption of soluble xylan biopolymers onto cellulosic material.

    Science.gov (United States)

    Chimphango, Annie F A; Görgens, J F; van Zyl, W H

    2016-06-05

    The functional properties of cellulose fibers can be modified by adsorption of xylan biopolymers. The adsorption is improved when the degree of biopolymers substitution with arabinose and 4-O-methyl-glucuronic acid (MeGlcA) side groups, is reduced. α-l-Arabinofuranosidase (AbfB) and α-d-glucuronidase (AguA) enzymes were applied for side group removal, to increase adsorption of xylan from sugarcane (Saccharum officinarum L) bagasse (BH), bamboo (Bambusa balcooa) (BM), Pinus patula (PP) and Eucalyptus grandis (EH) onto cotton lint. The AguA treatment increased the adsorption of all xylans by up to 334%, whereas, the AbfB increased the adsorption of the BM and PP by 31% and 44%, respectively. A combination of AguA and AbfB treatment increased the adsorption, but to a lesser extent than achieved with AguA treatment. This indicated that the removal of the glucuronic acid side groups provided the most significant increase in xylan adsorption to cellulose, in particular through enzymatic treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. PRODUCTION OF BIOETHANOL FROM AGRICULTURAL WASTE

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    W. Braide

    2016-05-01

    Full Text Available This study investigates the potential of ethanol production from agro wastes. Agro waste from sugarcane Saccharum officinarum (sugarcane baggasse, sugarcane bark and maize plant Zea mays (corncob, corn stalk, corn husk was subjected to a pretreatment process using acid hydrolysis was applied to remove lignin which acts as physical barrier to cellulolytic enzymes. Ethanolic fermentation was done using Saccharomyces cerevisiae for 5days and the ethanol yield, specific gravity, pH and total reducing sugar were also determined. From the results, the specific gravity, sugar content and pH decreased over time while the Sugarcane baggasse, Sugarcane bark, Cornstalk, Corncob and Cornhusk gave maximum percentage ethanol yield of 6.72, 6.23, 6.17, 4.17 and 3.45 respectively at 72hrs Fermentation. Maximum yields of ethanol were obtained at pH 3.60, 3.82, 4.00, 3.64 and 3.65. These findings show/prove that ethanol can be made from the named agricultural waste and the process is recommended as a means of generating wealth from waste.

  2. Centesimal composition and physical-chemistry analysis of the edible mushroom Lentinus strigosus occurring in the Brazilian Amazon.

    Science.gov (United States)

    Sales-Campos, Ceci; Araujo, Lidia M; Minhoni, Marli T A; Andrade, Meire C N

    2013-01-01

    The centesimal composition and the physical and chemical analyses of Lentinus strigosus, an edible mushroom occurring in the Brazilian Amazon and produced in alternative substrates based on wood and agroindustrial residues, were evaluated. For this purpose, the C, N, pH, soluble solids, water activity, protein, lipids, total fiber, ash, carbohydrate, and energy levels were determined. The substrates were formulated from Simarouba amara Aubl. ("marupá"), Ochroma piramidale Cav. Ex. Lam. ("pau-de-balsa") and Anacardium giganteum ("cajuí") sawdust and Bactris gasipaes Kunth ("pupunheira") stipe and Saccharum officinarum (sugar cane bagasse). The results indicated that the nutritional composition of L. strigosus varied with the substrate of cultivation; the protein levels found in mushrooms grown in the different substrates (18-21.5%) varied with the substrate and was considered high; the soluble solids present in the mushrooms could have a relation with complex B hydrosoluble vitamins. L. strigosus could be considered as important food owing to its nutritional characteristics such as high protein content, metabolizable carbohydrates and fibers, and low lipids and calories content.

  3. Conservation of soil, water and nutrients in surface runoff using riparian plant species.

    Science.gov (United States)

    Srivastava, Prabodh; Singh, Shipra

    2012-01-01

    Three riparian plant species viz. Cynodon dactylon (L.) Pers., Saccharum bengalensis Retz. and Parthenium hysterophorus L. were selected from the riparian zone of Kali river at Aligarh to conduct the surface runoff experiment to compare their conservation efficiencies for soil, water and nutrients (phosphorus and nitrogen). Experimental plots were prepared on artificial slopes in botanical garden and on natural slopes on study site. Selected riparian plant species showed the range of conservation values for soil and water from 47.11 to 95.22% and 44.06 to 72.50%, respectively on artificial slope and from 44.53 to 95.33% and 48.36 to 73.15%, respectively on natural slope. Conservation values for phosphorus and nitrogen ranged from 40.83 to 88.89% and 59.78 to 82.22%, respectively on artificial slope and from 50.01 to 90.16% and 68.07 to 85.62%, respectively on natural slope. It was observed that Cynodon dactylon was the most efficient riparian species in conservation of soil, water and nutrients in surface runoff.

  4. Utilization of vinasses as soil amendment: consequences and perspectives.

    Science.gov (United States)

    Moran-Salazar, R G; Sanchez-Lizarraga, A L; Rodriguez-Campos, J; Davila-Vazquez, G; Marino-Marmolejo, E N; Dendooven, L; Contreras-Ramos, S M

    2016-01-01

    Vinasses are a residual liquid generated after the production of beverages, such as mezcal and tequila, from agave (Agave L.), sugarcane (Saccharum officinarum L.) or sugar beet (Beta vulgaris L.). These effluents have specific characteristics such as an acidic pH (from 3.9 to 5.1), a high chemical oxygen demand (50,000-95,000 mg L(-1)) and biological oxygen demand content (18,900-78,300 mg L(-1)), a high total solids content (79,000 and 37,500 mg L(-1)), high total volatile solids 79,000 and 82,222 mg L(-1), and K(+) (10-345 g L(-1)) content. Vinasses are most commonly discarded onto soil. Irrigation of soil with vinasses, however, may induce physical, chemical and biochemical changes and affect crop yields. Emission of greenhouse gases (GHG), such as carbon dioxide, nitrous oxide and methane, might increase from soils irrigated with vinasses. An estimation of GHG emission from soil irrigated with vinasses is given and discussed in this review.

  5. Production of sugarcane and tropical grasses as a renewable energy source. Third quarterly report, December 1, 1979-February 29, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Alexander, A G; Allison, W; Garcia, M; Ramirez, G; Chu, T L; Velez-Santiago, J; Smith, L

    1980-01-01

    Research continued on tropical grasses from Saccharum and related genera as sources of intensively-propagated fiber and fermentable solids. Both complete and incomplete data from the first three quarters of year 3 were reviewed. Candidate screening (for short-rotation grasses) was expanded to include six sorghum x Sudan grass hybrids developed by the Dekalb Company. Incomplete data indicate that two of the new grasses might be comparable to the NK hybrids in total biomass production and drought tolerance. Sugarcane and napier grass yield trends in year 3 includes: (a) Increased yields with delay of harvest frequency; (b) lxck of response to close spacing; (c) a superiority of napier grass over sugarcane when harvested at intervals of six months or less; and (d), a general superiority of the sugarcane variety NCo 310 over varieties PR 980 and PR 64-1791. Delayed tasseling of a wild, early-flowering S. spontaneous hybrid enabled three crosses to be made in December using commercial hybrids as female parents. Approximately 1000 seedlings were produced.

  6. NITROGEN MANAGEMENT IN SUGARCANE AND ITS INFLUENCE ON YIELD, PROFITABILITY AND LEACHING LOSSES

    Directory of Open Access Journals (Sweden)

    Juan Carlos Moreno-Seceña

    2011-11-01

    Full Text Available The excessive use of nitrogen (N in sugarcane (Saccharum officinarum L. is a source of contamination for aquifers. The objective was to evaluate sugarcane yield, as well as profitability and amount of N leached resulting from the application of different split N doses. Three N doses (250, 200 and 150 kg ha-1 and three different application numbers (2, 3 and 4 were evaluated using a factorial design in randomized blocks. When the N dose was divided in three and four applications yields higher than 125 ton ha-1 were obtained. The greatest benefit-cost-ratio (1.8 resulted from using 150 kg ha-1 of N divided in three applications. The lowest N losses due to leaching were obtained using 150 kg ha-1 of N divided in three (16.8 kg ha-1 and four (15.4 kg ha-1 applications. Low N doses divided in three or four applications did not reduce sugarcane production; furthermore, it was more profitable and had a lower environmental impact by reducing N leaching.

  7. Haplotype structure around Bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars.

    Science.gov (United States)

    Costet, L; Le Cunff, L; Royaert, S; Raboin, L-M; Hervouet, C; Toubi, L; Telismart, H; Garsmeur, O; Rousselle, Y; Pauquet, J; Nibouche, S; Glaszmann, J-C; Hoarau, J-Y; D'Hont, A

    2012-09-01

    Modern sugarcane cultivars (Saccharum spp., 2n = 100-130) are high polyploid, aneuploid and of interspecific origin. A major gene (Bru1) conferring resistance to brown rust, caused by the fungus Puccinia melanocephala, has been identified in cultivar R570. We analyzed 380 modern cultivars and breeding materials covering the worldwide diversity with 22 molecular markers genetically linked to Bru1 in R570 within a 8.2 cM segment. Our results revealed a strong LD in the Bru1 region and strong associations between most of the markers and rust resistance. Two PCR markers, that flank the Bru1-bearing segment, were found completely associated with one another and only in resistant clones representing efficient molecular diagnostic for Bru1. On this basis, Bru1 was inferred in 86 % of the 194 resistant sugarcane accessions, revealing that it constitutes the main source of brown rust resistance in modern cultivars. Bru1 PCR diagnostic markers should be particularly useful to identify cultivars with potentially alternative sources of resistance to diversify the basis of brown rust resistance in breeding programs.

  8. Optimized Subsurface Irrigation System: The Future of Sugarcane Irrigation

    Directory of Open Access Journals (Sweden)

    M. H. J. P. Gunarathna

    2018-03-01

    Full Text Available Climate change may harm the growth and yield of sugarcane (Saccharum officinarum L. without the introduction of appropriate irrigation facilities. Therefore, new irrigation methods should be developed to maximize water use efficiency and reduce operational costs. OPSIS (optimized subsurface irrigation system is a new solar-powered automatic subsurface irrigation system that creates a phreatic zone below crop roots and relies on capillarity to supply water to the root zone. It is designed for upland crops such as sugarcane. We investigated the performance of OPSIS for irrigating sugarcane and evaluated its performance against sprinkler irrigation under subtropical conditions. We conducted field experiments in Okinawa, Japan, over the period from 2013 to 2016 and took measurements during spring- and summer-planted main crops and two ratoon crops of the spring-planted crop. Compared with sprinkler irrigation, OPSIS produced a significantly higher fresh cane yield, consumed less irrigation water and provided a higher irrigation water use efficiency. We conclude that OPSIS could be adopted as a sustainable solution to sugarcane irrigation in Okinawa and similar environments.

  9. Water stress indices for the sugarcane crop on different irrigated surfaces

    Directory of Open Access Journals (Sweden)

    Rodrigo G. Brunini

    Full Text Available ABSTRACT Sugarcane (Saccharum officinarum L. is a crop of vital importance to Brazil, in the production of sugar and ethanol, power generation and raw materials for various purposes. Strategic information such as topography and canopy temperature can provide management technologies accessible to farmers. The objective of this study was to determine water stress indices for sugarcane in irrigated areas, with different exposures and slopes. The daily water stress index of the plants and the water potential in the soil were evaluated and the production system was analyzed. The experiment was carried out in an “Experimental Watershed”, using six surfaces, two horizontal and the other ones with 20 and 40% North and South exposure slopes. Water stress level was determined by measuring the temperatures of the vegetation cover and the ambient air. Watering was carried out using a drip irrigation system. The results showed that water stress index of sugarcane varies according to exposure and slope of the terrain, while areas whose water stress index was above 5.0 oC had lower yield values.

  10. Effect of Azospirillum brasilense on the harvest and radical development of sugar cane plants, variety C86 - 456, obtained by in vitro culture in normal conditions and under overhumidity of soil

    Directory of Open Access Journals (Sweden)

    Sergio Rodríguez Rodríguez

    2005-01-01

    Full Text Available In order to know the influence of the bacteria Azospirillum brasilense on plants of sugar cane, cultivar C86-456, from tissue culture under normal conditions and flooded soil in a Vertisol from El Valle del Cauto, an experiment was carried out using an at random blocks design. The main crop variables (pol in cane, t. caña.ha-1 and t. pol.ha-1 at the twelve months of age were taken, as stump of spring of the year and the development reached by the radical system in its more active area to achieve this experiment. The best results in the crop variables and development of the radical system were obtained it the treatments where the bacteria was present, although non significant under both conditions, evidencing that the stress due to excess of water in the soil affects the normal development of the sugar cane in general by modifying the kindness that the rhizospheric microorganism provides. Key words: biofertilizer, radical system, Saccharum, yield

  11. Are sugarcane leaf-detritus well colonized by aquatic macroinvertebrates?

    Directory of Open Access Journals (Sweden)

    Luciene Aparecida Leite-Rossi

    Full Text Available AIM: The aim was to compare the kinetics of decomposition and the colonization of leaf litter of two plant species, the native Talauma ovata (pinha-do-brejo and the exotic Saccharum officinarum (sugarcane, by aquatic macroinvertebrates; METHODS: From each substrate, three recipients of colonization were taken from a stream, and the specimens identified to the lowest taxonomic level on days 7, 15, 34, 44, 61 and 75. The debris was weighed at the beginning and end of the experiment and determined their cell wall fractions; RESULTS: The coefficients of mineralization indicated higher velocity decay of organic matter refractory in T. ovata. There was no difference in taxonomic structure of macroinvertebrates, between the two substrates, but the community exhibited distinct functional feeding groups in the peak of colonization, with a greater number of shredders in T. ovata. The successive states of decomposition of the two plant detritus showed distinct macroinvertebrate densities; CONCLUSIONS: The amount and state of the plant biomass were important factors influencing the density and diversity of the macroinvertebrate fauna throughout the process of organic decomposition.

  12. Pérdidas de suelo y nutrientes bajo diferentes coberturas vegetales en la zona Andina de Colombia Soil and nutrient loss under different vegetation covers in Colombia's Andean region

    Directory of Open Access Journals (Sweden)

    J. Alexander Rodríguez

    2009-07-01

    Full Text Available El estudio se realizó en la reserva natural El Ciprés, ubicada en la vereda Bellavista, municipio de El Dovio (Valle del Cauca. La vereda se encuentra en la zona Andina de la cordillera occidental, en el departamento del Valle del Cauca, entre 1700 y 1800 m.s.n.m., con una temperatura promedio de 18 °C. Según la clasificación climática de Holdridge, corresponde a una zona de bosque húmedo Montano Bajo, con una precipitación promedio entre 2500 y 2700 mm/año, una humedad relativa de 90% y una pendiente del suelo de 62%. Las mediciones se hicieron en parcelas de escorrentía de 32 m² cada una y siete tipos de coberturas: guadua (Guadua angustifolia Kunth, bosque secundario, pastura (Brachiaria decumbens, café (Coffea arabica; banco de proteína (Trichanthera gigantea, caña forrajera (Saccharum officinarum y cultivo limpio conformado por yuca (Manihot esculenta, maíz (Zea mays y arracacha (Arracacia zanthorrhiza Brancroft. Para la evaluación se midieron las pérdidas de suelo y los nutrientes calcio, magnesio, potasio y fósforo en un periodo de 7 meses. Los resultados mostraron diferencias (P Soil and nutrient (calcium, magnesium, potassium, phosphorus losses were measured over a 7-month period in the El Ciprés Natural Reserve, located in the Bellavista rural community, municipality of El Dovio, in the western cordillera of the Andes of the department of Valle del Cauca, Colombia. At 1700-1800 meters above sea level, the area presents an average temperature of 18 °C, an average annual precipitation of 2500-2700 mm, 90% relative humidity, and a 62% slope. According to the Holdridge climate classification system, it corresponds to a lower montane rain forest. Measurements were taken in runoff plots, each 32 m², with seven types of vegetation cover: giant bamboo (Guadua angustifolia Kunth; secondary forest; pastures (Brachiaria decumbens; coffee (Coffea arabica; protein bank (Trichanthera gigantea; forage cane (Saccharum officinarum

  13. Estresse salino em duas variedades de cana-de-açúcar: enzimas do sistema antioxidativo e fluorescência da clorofila Salinity stress in two varieties of sugar cane: enzymes of the antioxidant system and chlorophyll fluorescence

    Directory of Open Access Journals (Sweden)

    Lilia Willadino

    2011-06-01

    Full Text Available O Brasil destaca-se por ser o maior produtor de cana-de-açúcar (Saccharum officinarum L. e por dominar todos os estágios da tecnologia de produção de açúcar e etanol. Atualmente se observa a expansão dessa cultura para a região semiárida brasileira, a qual apresenta solos propensos à salinização. O presente trabalho objetivou avaliar a resposta à salinidade de duas variedades de cana-de-açúcar. O ensaio foi implantado em casa de vegetação, em delineamento inteiramente casualizado, em esquema fatorial 2 x 8 com cinco repetições, correspondendo a duas variedades de cana-de-açúcar (RB867515 e RB863129 e oito níveis de NaCl (0; 25; 50; 75; 100; 125; 150; 200 mM de NaCl. A salinidade induziu aumento na fluorescência e diminuição na concentração de clorofila em ambas as variedades. A redução da clorofila, entretanto, foi maior na variedade RB867515. Observou-se, nesta mesma variedade, aumento da atividade da peroxidase do ascorbato (APX e da catalase (CAT, enquanto na variedade RB863129 ocorreu redução na atividade dessas enzimas. Esses resultados indicam que a variedade RB867515 apresenta um mecanismo mais eficiente na proteção da planta contra danos no aparato fotoquímico e contra a ação de espécies reativas de oxigênio, em particular o H2O2.Brazil is seen as being the largest producer of sugarcane (Saccharum officinarum L. and of mastering all stages in the production of sugar and ethanol. Currently this production is being expanded into the semi-arid regions of Brazil, where there are soil-types prone to salinity. This study aimed to evaluate the response to salinity in two sugarcane genotypes, RB867515 and RB863129. Salinity caused an increase in chlorophyll fluorescence and a reduction of chlorophyll concentration in both varieties. The reduction of chlorophyll however was higher in RB867515. In this same type, an increase in the activity of ascorbate peroxidase (APX and catalase (CAT was observed, while in

  14. Chemical composition and fermentative parameters of elephant grass and sugar-cane silage treated with additives Composição química e parâmetros fermentativos de silagens de capim-elefante e cana-de-açúcar tratadas com aditivos

    Directory of Open Access Journals (Sweden)

    Elson Martins Coelho

    2010-09-01

    Full Text Available It was aimed to evaluate the effect of different additives in the elephant-grass (Pennisetum purpureum Schum. and sugar-cane (Saccharum officinarum L., before and after the ensilage, through fermentation and chemical composition measures. The roughages were ensiled in PVC laboratory silos, with three for treatment, totalizing 24 microsilos for specie, in a completely randomized design. The treatments were control (without additive, corn meal, common salt, urea, acetic acid, formic acid, microbial inoculant and dried molasses. Samples were taken before and after 35 days of ensilage. Dry matter, crude protein and ammonia-N contents, pH and buffering capacity values and dry matter losses were analyzed. The elephant-grass presented satisfactory results for conservation in the silage form, and it was not necessary the use of additives. The sugar-cane presented satisfactory results for conservation in the silage form. The corn meal was the additive that promoted best fermentation pattern and smaller losses, as an option in the making of the sugar-cane silage.Objetivou-se avaliar o efeito de aditivos no capim-elefante (Pennisetum purpureum Schum. e na cana-de-açúcar (Saccharum officinarum L., antes e após a ensilagem, através de medidas de fermentação e composição química. As forragens foram ensiladas em silos laboratoriais de PVC, com três unidades por tratamento, num total de 24 microsilos por espécie forrageira, em delineamento inteiramente casualizado. Os tratamentos consistiram em controle (sem aditivo, fubá, cloreto de sódio, ureia, ácido acético, ácido fórmico, inoculante microbiano e melaço em pó. Amostras foram tomadas antes e após 35 dias de ensilagem, de modo que foram analisados os teores de matéria seca (MS, proteína bruta e nitrogênio amoniacal, os valores de pH e capacidade tamponante, além das perdas de matéria seca. O capim-elefante apresentou resultados satisfatórios para conservação na forma de silagem

  15. Are sugarcane leaf-detritus well colonized by aquatic macroinvertebrates? Detritos foliares de cana-de-açúcar são bem colonizados por macroinvertebrados aquáticos?

    Directory of Open Access Journals (Sweden)

    Luciene Aparecida Leite-Rossi

    2012-01-01

    Full Text Available AIM: The aim was to compare the kinetics of decomposition and the colonization of leaf litter of two plant species, the native Talauma ovata (pinha-do-brejo and the exotic Saccharum officinarum (sugarcane, by aquatic macroinvertebrates; METHODS: From each substrate, three recipients of colonization were taken from a stream, and the specimens identified to the lowest taxonomic level on days 7, 15, 34, 44, 61 and 75. The debris was weighed at the beginning and end of the experiment and determined their cell wall fractions; RESULTS: The coefficients of mineralization indicated higher velocity decay of organic matter refractory in T. ovata. There was no difference in taxonomic structure of macroinvertebrates, between the two substrates, but the community exhibited distinct functional feeding groups in the peak of colonization, with a greater number of shredders in T. ovata. The successive states of decomposition of the two plant detritus showed distinct macroinvertebrate densities; CONCLUSIONS: The amount and state of the plant biomass were important factors influencing the density and diversity of the macroinvertebrate fauna throughout the process of organic decomposition.OBJETIVO: O principal objetivo foi comparar a cinética de decomposição e a colonização de detritos de duas espécies de plantas: a nativa Talauma ovata (pinha-do-brejo e a exótica Saccharum officinarum (cana-de-açúcar por macroinvertebrados; MÉTODOS: Para cada substrato, três recipientes de colonização foram retirados de um riacho e os espécimes identificados até o menor nível taxonômico nos dias 7, 15, 34, 44, 61 e 75. Os detritos foram pesados no início e ao final do experimento e determinadas suas frações de parede celular; RESULTADOS: Os coeficientes de mineralização indicaram maior velocidade de decaimento da matéria orgânica refratária nos detritos de T. ovata. Os dois substratos não apresentaram diferenças na estrutura taxonômica da comunidade

  16. Are sugarcane leaf-detritus well colonized by aquatic macroinvertebrates? Detritos foliares de cana-de-açúcar são bem colonizados por macroinvertebrados aquáticos?

    Directory of Open Access Journals (Sweden)

    Luciene Aparecida Leite-Rossi

    2012-09-01

    Full Text Available AIM: The aim was to compare the kinetics of decomposition and the colonization of leaf litter of two plant species, the native Talauma ovata (pinha-do-brejo and the exotic Saccharum officinarum (sugarcane, by aquatic macroinvertebrates; METHODS: From each substrate, three recipients of colonization were taken from a stream, and the specimens identified to the lowest taxonomic level on days 7, 15, 34, 44, 61 and 75. The debris was weighed at the beginning and end of the experiment and determined their cell wall fractions; RESULTS: The coefficients of mineralization indicated higher velocity decay of organic matter refractory in T. ovata. There was no difference in taxonomic structure of macroinvertebrates, between the two substrates, but the community exhibited distinct functional feeding groups in the peak of colonization, with a greater number of shredders in T. ovata. The successive states of decomposition of the two plant detritus showed distinct macroinvertebrate densities; CONCLUSIONS: The amount and state of the plant biomass were important factors influencing the density and diversity of the macroinvertebrate fauna throughout the process of organic decomposition.OBJETIVO: O principal objetivo foi comparar a cinética de decomposição e a colonização de detritos de duas espécies de plantas: a nativa Talauma ovata (pinha-do-brejo e a exótica Saccharum officinarum (cana-de-açúcar por macroinvertebrados; MÉTODOS: Para cada substrato, três recipientes de colonização foram retirados de um riacho e os espécimes identificados até o menor nível taxonômico nos dias 7, 15, 34, 44, 61 e 75. Os detritos foram pesados no início e ao final do experimento e determinadas suas frações de parede celular; RESULTADOS: Os coeficientes de mineralização indicaram maior velocidade de decaimento da matéria orgânica refratária nos detritos de T. ovata. Os dois substratos não apresentaram diferenças na estrutura taxonômica da comunidade

  17. Responses of secondary chemicals in sugar maple (Acer saccharum) seedlings to UV-B, springtime warming and nitrogen additions

    Energy Technology Data Exchange (ETDEWEB)

    Sager, E.P.S.; Hutchinson, T.C. [Trent Univ., Peterborough, ON (Canada). Environmental Studies

    2006-10-15

    Elevated UV-B radiation due to climatic change and ozone depletion may represent a significant springtime environmental stressor to germinating seedlings in temperate forest regions. This study aimed to determine the effects of UV-B, nitrogen (N) fertilization and climate warming on the concentrations of base cations and secondary metabolites in the foliage of sugar maple seedlings growing in acid or alkaline soils. The influence of measured flavonoids and phenolics on herbivore activity was examined, as well as the relationship between foliar concentrations of calcium (Ca); manganese (Mn); and N and the production of phenolic and flavonoid compounds. Experimental plots were established in mature hardwood forests in alkaline and acid soil locations in Bobcaygeon and Haliburton, Ontario. Pentagonal open-top chambers were used to lengthen the growing season and simulate an earlier spring. Ammonium nitrate was applied at a rate comparable with an additional deposition of 5 g N per m per year. Fertilizer was applied on 3 separate occasions. Ambient UV-B radiation was screened out with Mylar D polyester film. Sites, treatments and time of sampling had complex effects on foliar elemental chemistry, production of secondary compounds and herbivory. Foliar concentrations of individual phenols were higher in seedlings in the UV-B exclusion treatments. At both sites, removal of ambient UV-B led to increases in flavonoids and chlorogenic acid, and reduced herbivore activity. At Haliburton, ammonium nitrate fertilization led to further increases in foliar Mn. Nitrogen additions led to decreases in the concentrations of some flavonoids at both sites. It was concluded that the composition of the forest soil governs the response of seedlings when they are exposed to abiotic stressors. 63 refs., 5 tabs., 8 figs.

  18. Discovering the desirable alleles contributing to the lignocellulosic biomass traits in Saccharum germplasm collections for energy cane improvement

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jianping [Univ. of Florida, Gainesville, FL (United States); Sandhu, Hardev [Univ. of Florida, Gainesville, FL (United States)

    2017-03-23

    1) The success in crop improvement programs depends largely on the extent of genetic variability available. Germplasm collections assembles all the available genetic resources and are critical for long-term crop improvement. This world sugarcane germplasm collection contains enormous genetic variability for various morphological traits, biomass yield components, adaptation and many quality traits, prospectively imbeds a large number of valuable alleles for biofuel traits such as high biomass yield, quantity and quality of lignocelluloses, stress tolerance, and nutrient use efficiency. The germplasm collection is of little value unless it is characterized and utilized for crop improvement. In this project, we phenotypically and genotypically characterized the sugarcane world germplasm collection (The results were published in two papers already and another two papers are to be published). This data will be made available for public to refer to for germplasm unitization specifically in the sugarcane and energy cane breeding programs. In addition, we are identifying the alleles contributing to the biomass traits in sugarcane germplasm. This part of project is very challenging due to the large genome and highly polyploid level of this crop. We firstly established a high throughput sugarcane genotyping pipeline in the genome and bioinformatics era (a paper is published in 2016). We identified and modified a software for genome-wide association analysis of polyploid species. The results of the alleles associated to the biomass traits will be published soon, which will help the scientific community understand the genetic makeup of the biomass components of sugarcane. Molecular breeders can develop markers for marker assisted selection of biomass traits improvement. Further, the development and release of new energy cane cultivars through this project not only improved genetic diversity but also improved dry biomass yields and resistance to diseases. These new cultivars were tested on marginal soils in Florida and showed very promising yield potential that is important for the successful use of energy cane as a dedicated feedstock for lignocellulosic ethanol production. 2) Multiple techniques at different project progress stages were utilized. For example, for the whole world germplasm accession genotyping, a cheap widely used SSR marker genotyping platform was utilized due to the large number of samples (over thousand). But the throughput of this technique is low in generating data points. However, the purpose the genotyping is to form a core collection for further high throughput genotyping. Thus the results from the SSR genotyping was quite good enough to generated the core collection. To genotype the few hundred core collection accessions, an target enrichment sequencing technology was used, which is not only high throughput in generating large number of genotyping data, but also has the candidate genes targeted to genotyping. The data generated would be sufficient in identifying the alleles contributing to the traits of interests. All the techniques used in this project are effective though extensive time was invested specifically for establish the pipeline in the experimental design, data analysis, and different approach comparison. 3) the research can benefit to the public in polyploid genotyping and new and cost efficient genotyping platform development

  19. Negative cross resistance in atrazine resistant junglerice (Echinochloa colona (L. Link populations in sugarcane (Saccharum officinarum L. fields

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    Elham Elahifard

    2018-01-01

    Full Text Available Introduction Negative and positive cross-resistance to other herbicides have been found in triazine-resistant biotypes. Thus, negative cross-resistance has been reported to bentazone for Brassica napus L. and A. retroflexus L., and to pyridate for B. napus and Epilobium ciliatum Raf.. In contrast, positive cross-resistance to bentazone has been found in Chenopodium album L. and Solanum nigrum L.. Negative cross-resistance, i.e., herbicide-resistant plants being more sensitive to herbicides than susceptible plants, has been documented in several triazine-resistant weed biotypes. The goal of this study was to search for herbicides that exert negative cross-resistance at the whole-plant level for control of atrazine-resistant populations of E. colona. Materials and Methods Suspected resistant and susceptible seeds of junglerice to atrazine were collected from sugarcane fields and adjacent areas of Karun Agro-Industry Inc., Shushtar, southwestern of Iran in 2014 - 2015 growing season. These populations were named according to their population status and abbreviated as follows: R1, R2, R3 and S (susceptible population. All the collected seeds (R- and S-populations were stored at room temperature (25 oC. Herbicides were tebuthiuron, linuron, diuron and diuron + hexazinone. For the PRE application, 10 seeds were planted in 500-ml plastic pots containing clay:sand mix, and herbicides were applied using an flood nozzle and back sprayer calibrated to deliver 350 L ha-1 to the suspected resistant and susceptible junglerice biotypes 1 d after sowing. Plants were thinned to 4 plants per pot. The aboveground biomass was harvested 28 DAT, dried at 75 °C for 48 h, and weighed. The aboveground biomass data were expressed as a percentage of the untreated control. The experiment was a completely randomized block design with four replications and was repeated. The data were analyzed using a nonlinear regression model and R software (drc add on packeges, the three and four parameters log-logistic equation was fitted to the data to describe the responses of the populations to herbicides: susceptible population was estimated as an index in order to compare the resistance levels of the tested populations. Results and Discussion The result of screening test showed that 3 populations were resistant to atrazine. The results of dose-response assay using atrazine revealed that resistance factor of R1, R2 and R3 populations were 12.26, 6.59 and 3.75 based on wet weight (% of control and 5.37, 4.22 and 4.71 based on number of survival plants (% of control, respectively. The ED50 values of the R1, R2 and R3 populations were 36.85, 73.01 and 41.71 g ai ha-1 compared with 44.42 g ai ha-1 of the S-populaton of tebuthiuron. The Rf of the R1, R2 and R3 populations were 0.80, 1.53 and 0.72 of tebuthiuron, respectively. The ED50 values of the R1, R2 and R3 populations were 92.76, 107.73 and 106.84 g ai ha-1 compared with 152.51 g ai ha-1 of the S-populaton of linuron. The Rf of the R1, R2 and R3 populations were 1, 1.89 and 3.26 of tebuthiuron, respectively. The ED50 values of the R1, R2 and R3 populations were 74.21, 95.25 and 69.80 g ai ha-1 compared with 79.03 g ai ha-1 of the S-populaton of diuron. The Rf of the R1, R2 and R3 populations were 0.97, 0.85 and 1.18 of diuron, respectively. The ED50 values of the R1, R2 and R3 populations were 62.11, 49.48 and 54.30 g ai ha-1 compared with 88.72 g ai ha-1 of the S-populaton of diuron+hexazinone. The Rf of the R1, R2 and R3 populations were 0.62, 0.68 and 0.52 of diuron+hexazinone, respectively. The results showed that negative cross resistance to tebuthiuron, linuron, diuron and diuron + hexazinone. Resistant biotypes, also, showed the highest negative cross resistance to diuron+hexazinone. Conclusions Some herbicides that inhibit photosystem II bind more efficiently to the mutant triazine binding domain than to the wild (susceptible type. Triazine-resistant weeds frequently show negative cross-resistance to other photosystem-II inhibitors, such as bentazon and pyridate; triazine-resistant weeds can also exhibit negative cross-resistance to herbicides that do not affect photosystem II. Negative cross resistance may be the major reason that atrazine resistance did not evolve where herbicide mixtures were used, when the mixed herbicide (usually a non-PS II inhibiting acetanilide also controlled triazine-sensitive weeds. The value of negative cross-resistance linked with the general lack of fitness of almost all triazine-resistant weeds may be greater than we measured herein. The competition exerted by a crop in the field may further accentuate and exacerbate the lack of fitness and further lower the RI.

  20. KRAFT PULPING CHARACTERISTICS OF THREE MOROCCAN EUCALYPTI. PART 2. COMPARISON OF THE GUAIACYL FRACTION OF THE NATIVE LIGNINS BY A NOVEL METHOD

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    Ericka F. Alves,

    2012-02-01

    Full Text Available In Part 1 of this series it was observed that one of the eucalypti (EGC 39 was more reactive than the other two in kraft and soda-AQ (SAQ cooking. However, the lignin in EGC 39 contained equal or less syringyl (S units than the other two eucalypti. In the present research an attempt was made to compare the guaiacyl (G fraction of the three lignins. The approach was to use SAQ treatment to cleave β-O-4 bonds in dimeric units containing uncondensed guaiacyl A-rings (those rearranging to quinone methides. The coniferyl alcohol, vinylguaiacol and isoeugenol generated from β-O-4 cleavage are then trapped as dimers by ethylguaiacol that is included in the SAQ liquor. Research with sugar maple (Acer saccharum showed that the estimate of these structures (uncondensed G-β-O-4 by this approach was in close agreement with traditional but more tedious methods such as permanganate oxidation and 31P NMR. It was also shown that the lignin in the EGC 39 hybrid contained a higher concentration of uncondensed G-β-O-4 structures than the other two eucalypti lignins.

  1. Plant Species Rather Than Climate Greatly Alters the Temporal Pattern of Litter Chemical Composition During Long-Term Decomposition

    Science.gov (United States)

    Li, Yongfu; Chen, Na; Harmon, Mark E.; Li, Yuan; Cao, Xiaoyan; Chappell, Mark A.; Mao, Jingdong

    2015-10-01

    A feedback between decomposition and litter chemical composition occurs with decomposition altering composition that in turn influences the decomposition rate. Elucidating the temporal pattern of chemical composition is vital to understand this feedback, but the effects of plant species and climate on chemical changes remain poorly understood, especially over multiple years. In a 10-year decomposition experiment with litter of four species (Acer saccharum, Drypetes glauca, Pinus resinosa, and Thuja plicata) from four sites that range from the arctic to tropics, we determined the abundance of 11 litter chemical constituents that were grouped into waxes, carbohydrates, lignin/tannins, and proteins/peptides using advanced 13C solid-state NMR techniques. Decomposition generally led to an enrichment of waxes and a depletion of carbohydrates, whereas the changes of other chemical constituents were inconsistent. Inconsistent convergence in chemical compositions during decomposition was observed among different litter species across a range of site conditions, whereas one litter species converged under different climate conditions. Our data clearly demonstrate that plant species rather than climate greatly alters the temporal pattern of litter chemical composition, suggesting the decomposition-chemistry feedback varies among different plant species.

  2. Levantamento etnobotânico de plantas medicinais na comunidade quilombola Carreiros, Mercês – Minas Gerais

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    Flávia M. C. Ferreira

    2014-09-01

    Full Text Available O objetivo do trabalho foi identificar as espécies medicinais utilizadas pela comunidade quilombola Carreiros, bem como traçar o perfil social das famílias desta comunidade. Para tanto, foram realizadas entrevistas semiestruturadas da comunidade, sendo um representante de cada família. Para fins de registro foram coletados dois exemplares de cada espécie medicinal identificada, os quais foram conservados na forma de exsicatas e mantidos no Laboratório de Ecologia do IF Sudeste MG – Campus Rio Pomba. As mulheres aparentemente desempenham importante papel social e estão bastante envolvidas no conhecimento das plantas medicinais. Nota-se, que o conhecimento existente foi adquirido de forma empírica e éevidente entre as famílias a preocupação em repassar os conhecimentos tradicionais sobre o uso das plantas medicinais aos jovens. Dentre as principais espécies utilizadas pela comunidade estão: Citrus Sinensis L., Psidium guajava L., Leonurus sibiricus L., Citrus Limonia (Osbeck, Carica sp., Vernonia condensataBacker, Sechuium edule (Jacq. Sw, Sansevieria trifasciata Prain, Malpighia glabra L., e Saccharum zofficinarum L..

  3. A genomic approach to elucidating grass flower development

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    Dornelas Marcelo C.

    2001-01-01

    Full Text Available In sugarcane (Saccharum sp as with other species of grass, at a certain moment of its life cycle the vegetative meristem is converted into an inflorescence meristem which has at least two distinct inflorescence branching steps before the spikelet meristem terminates in the production of a flower (floret. In model dicotyledonous species such successive conversions of meristem identities and the concentric arrangement of floral organs in specific whorls have both been shown to be genetically controlled. Using data from the Sugarcane Expressed Sequence Tag (EST Project (SUCEST database, we have identified all sugarcane proteins and genes putatively involved in reproductive meristem and flower development. Sequence comparisons of known flower-related genes have uncovered conserved evolutionary pathways of flower development and flower pattern formation between dicotyledons and monocotyledons, such as some grass species. We have paid special attention to the analysis of the MADS-box multigene family of transcription factors that together with the APETALA2 (AP2 family are the key elements of the transcriptional networks controlling plant reproductive development. Considerations on the evolutionary developmental genetics of grass flowers and their relation to the ABC homeotic gene activity model of flower development are also presented.

  4. Tree-shoot elongation patterns in a gamma-irradiated northern forest community

    International Nuclear Information System (INIS)

    Buech, R.R.; Salmonson, B.J.

    1977-01-01

    Shoot elongation in the upper crowns of seven tree species was studied in a gamma-irradiated northern forest community near Rhinelander, Wis. Observations on the pattern and duration of shoot elongation are presented for the irradiation (1972) and postirradiation (1973) growing seasons. The gymnosperm Abies balsamea was the most radiosensitive species. Significant alteration in pattern and duration was observed in 1973 at exposure rates of 4 to 25 r/20-hr day; 31 r/20-hr day was lethal. At the other extreme, 116 r/20-hr day produced no significant effects on Acer saccharum shoot elongation pattern or duration. Acer rubrum, Betula papyrifera, Populus tremuloides, Quercus rubra, and Tilia americana were intermediate in radiosensitivity. Observed responses to radiation were alteration in the elongation pattern, suppression of internodal elongation, and death. Effects of the 1972 growing-season exposure were most obvious in the subsequent growing seasons. Retardation of initial elongation was characteristic of all species. Cessation of elongation was variable, even within species (e.g., P. tremuloides). The results suggest that bud differentiation and morphology and dependency on food reserves contributed to the lag in manifestation of radiation damage. The resultant crown characteristics are described and explained

  5. Centesimal composition and physical-chemistry analysis of the edible mushroom Lentinus strigosus occurring in the Brazilian Amazon

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    CECI SALES-CAMPOS

    2013-10-01

    Full Text Available The centesimal composition and the physical and chemical analyses of Lentinus strigosus, an edible mushroom occurring in the Brazilian Amazon and produced in alternative substrates based on wood and agroindustrial residues, were evaluated. For this purpose, the C, N, pH, soluble solids, water activity, protein, lipids, total fiber, ash, carbohydrate, and energy levels were determined. The substrates were formulated from Simarouba amara Aubl. (“marupá”, Ochroma piramidale Cav. Ex. Lam. (“pau-de-balsa” and Anacardium giganteum (“cajuí” sawdust and Bactris gasipaes Kunth (“pupunheira” stipe and Saccharum officinarum (sugar cane bagasse. The results indicated that the nutritional composition of L. strigosus varied with the substrate of cultivation; the protein levels found in mushrooms grown in the different substrates (18 – 21.5% varied with the substrate and was considered high; the soluble solids present in the mushrooms could have a relation with complex B hydrosoluble vitamins. L. strigosus could be considered as important food owing to its nutritional characteristics such as high protein content, metabolizable carbohydrates and fibers, and low lipids and calories content.

  6. Molasses : dampak negatif pada ruminansia

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    yanuartono - yanuartono

    2017-07-01

    Full Text Available ABSTRACT Molasses is a by-product or end product of sugar cane (Saccharum officcinarum L. or sugar-beet (Beta vulgaris L. var. Conditiva resulting from the manufacture of raw or refined sugar and consisting of structural sugars, hemicelluloses and minerals. It is mainly used to improve appetite of animals or add extra energy when prices of molasses are lower than that of other energy sources. Molasses can be a key ingredient for cost effective management of feeds and pastures. Supplementing poor quality feeds with molasses will increase feed intake and improve palatability. However, molasses can be toxic if fed at ad libitum or free choice, therefore, it is recommended that molasses should be supplemented in a restrictive form. These molasses poisoning papers and the negative impacts arose from long-standing studies because the poisoning is now rare. However, although this time is rare but did not close the possibility will appear again, Especially if given in an uncontrolled amount. Thus, this paper aims to remind users of molasses in the livestock industry to be wiser in their use. Key words: Key words: molasses, sugar cane, palatability, toxic

  7. Acúmulo de metais pesados em solos cultivados com cana-de-açúcar pelo uso contínuo de adubação fosfatada e água de irrigação

    Directory of Open Access Journals (Sweden)

    J. F. G. P. Ramalho

    1999-12-01

    Full Text Available Com vistas em verificar se a utilização de fertilizantes fosfatados e irrigação com água poluída trazem elevação dos teores de metais pesados no solo, amostras de um Podzólico Amarelo e de dois Latossolos Amarelos, que receberam fertilizante fosfatado, desde 1968, e de um Cambissolo irrigado com água do rio Paraíba do Sul, desde 1978, cultivados com cana-de-açúcar (Saccharum spp. em Campos dos Goytacazes, RJ, foram analisadas quanto aos teores totais de Cd, Co, Cr, Cu, Mn, Ni, Pb e Zn. Os resultados mostraram que os solos que receberam fertilizantes fosfatados durante 25 anos apresentaram aumento significativo de Cd quando comparados com áreas-controle, sem, contudo, elevá-los a níveis críticos. O Cambissolo irrigado durante 15 anos apresentou aumentos significativos de Cd, Cr, Co, Cu, Ni e Pb, tendo os resultados da extração seqüencial mostrado que a maior percentagem desses elementos encontra-se na fração residual, de baixa biodisponibilidade e mobilidade.

  8. Binding capacity and root penetration of seven species selected for revegetation of uranium tailings at Jaduguda in Jharkhand, India

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Lal; Soni, Prafulla [Ecology and Environment Division, Forest Research Institute, Dehradun (India)

    2010-08-25

    Uranium from ores mined at the three mines - Jaduguda, Bhatin and Narwapahar (Jharkhand) - is processed in the mill and the waste emerges as tailings. The recorded radioactivity level in these tailings is very low, but to avoid any long-term effect of these tailings on the atmosphere, humans, cattle as well as native flora and fauna, the tailings are covered with 30 cm layer of soil. This reduces the gamma radiation and radon emission levels. However, to consolidate the soil covering the tailings on a sustainable basis, the area needs to be revegetated by plant species having shallow root systems, good conservation value and low canopy cover. Another important criterion for selection of species is that they should not have any ethnobotanical relevance to the surrounding villages. Considering these criteria, seven native plant species of forestry origin, viz. Colebrookea oppositifolia, Dodonaea viscosa, Furcraea foetida, Imperata cylindrica, Jatropha gossypifolia, Pogostemon benghalense and Saccharum spontaneum have been selected for experimental trials. We describe here the strategies adopted for consolidation of radioactivity in tailings, revegetation practices used and the ecological role of the selected species in consolidating the tailings. (author)

  9. Binding capacity and root penetration of seven species selected for revegetation of uranium tailings at Jaduguda in Jharkhand, India

    International Nuclear Information System (INIS)

    Singh, Lal; Soni, Prafulla

    2010-01-01

    Uranium from ores mined at the three mines - Jaduguda, Bhatin and Narwapahar (Jharkhand) - is processed in the mill and the waste emerges as tailings. The recorded radioactivity level in these tailings is very low, but to avoid any long-term effect of these tailings on the atmosphere, humans, cattle as well as native flora and fauna, the tailings are covered with 30 cm layer of soil. This reduces the gamma radiation and radon emission levels. However, to consolidate the soil covering the tailings on a sustainable basis, the area needs to be revegetated by plant species having shallow root systems, good conservation value and low canopy cover. Another important criterion for selection of species is that they should not have any ethnobotanical relevance to the surrounding villages. Considering these criteria, seven native plant species of forestry origin, viz. Colebrookea oppositifolia, Dodonaea viscosa, Furcraea foetida, Imperata cylindrica, Jatropha gossypifolia, Pogostemon benghalense and Saccharum spontaneum have been selected for experimental trials. We describe here the strategies adopted for consolidation of radioactivity in tailings, revegetation practices used and the ecological role of the selected species in consolidating the tailings. (author)

  10. Impact of Hot-Water Extraction on Acetone-Water Oxygen Delignification of Paulownia Spp. and Lignin Recovery

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    Chen Gong

    2014-02-01

    Full Text Available A hardwood-based biorefinery process starting with hot-water extraction (HWE is recommended in order to remove most of the hemicelluloses/xylans before further processing. HWE may be followed by delignification in acetone/water in the presence of oxygen (AWO for the production of cellulose and lignin. In this study, the HWE-AWO sequence was evaluated for its effectiveness at removing lignin from the fast-growing species Paulownia tomentosa (PT and Paulownia elongata (PE, in comparison with the reference species, sugar maple (Acer saccharum, SM. HWE might lead to a remarkable increase in lignin accessibility, and as a result, a greater AWO delignification degree was observed for extracted PT, PE, and SM than for unextracted ones. Organosolv lignin was recovered from the spent liquor of AWO delignification of PT with/without prior HWE and characterized to evaluate the benefits of HWE on the lignin structure and purity. The lignin recovered from the spent liquor of HWE-AWO sequence is of higher purity and lighter color than that recovered from the AWO spent liquor. These properties along with low sulfur content are desirable for lignin high-value applications.

  11. DESEMPENHO TÉRMICO DE BEZERREIROS COBERTOS COM TELHA DE FIBROCIMENTO E FORRO ECOLÓGICO DE SUBPRODUTOS AGROINDÚSTRIAS ASSOCIADO A MATERIAIS ISOLANTES

    Directory of Open Access Journals (Sweden)

    G. Barbirato

    2015-09-01

    Full Text Available Este trabalho descreve um estudo sobre o potencial de utilização de um forro ecológico constituído de subprodutos da agroindústria (partículas de madeira de maravalha de Pinus sp. e bagaço de cana-de-açúcar - Saccharum officinarum L. associado a placas de poliestireno expandido (isopor® e de poliuretano à base de óleo de mamona (PU-mamona, quando aplicados em bezerreiros cobertos com telha de fibrocimento. Nesse estudo foi aferida a temperatura interna, umidade relativa e o índice de temperatura e umidade (ITU, no período do verão, durante 21 dias. Os resultados obtidos indicaram que o ITU e temperatura ambiente interna das instalações com forro ecológico associado a placas de isopor® e PU-mamona foram inferiores àquelas aferidas em bezerreiros sem forro. As imagens captadas por câmera termográfica indicam que os materiais empregados como forro amorteceram a transferência de calor e radiação para o interior dos bezerreiros, ocasionando menores temperaturas internas nesses ambientes.

  12. The influence of altitude of the place on the vegetative growth of several varieties of sugarcane (saccharum officinarum l.)

    Science.gov (United States)

    Panataria, Lince R.; Lubis, Ramli

    2018-03-01

    This study is aimed at finding out vegetative growth of some varieties of sugarcane on different altitudes. The study applied Factorial Randomized Block Design comprising two factors. The first factor was varieties of consisting of three levels: BM 13579, Ps. 92-1, and Ps. 89-1. The second factor was altitudes of the land with three different levels of altitudes: Kebun Saentis (15 MASL), district of Pancurbatu (±300 MASL), and district of Sibolangit (±700 MASL). The study resulted in that variety of sugarcane, altitudes, and the interaction of both variables affected these: the leaf area on observations of 21, 42, 63, and 84 daps, number of tillers, root volume, nutrient uptake on observation 84 daps. The best quality of the sugarcane varieties was variety Ps. 92-1 on altitude 15 MASL (Kebun Saentis), followed by an altitude ±300 MASL (district of Pancurbatu), and then on ±700 MASL (district of Sibolangit); while the worst quality of the variety was variety BM 13579 of altitude 15 MASL (Kebun Saentis).

  13. Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies

    Science.gov (United States)

    da Silva-Froufe, Lúcia Gracinda; Boddey, Robert Michael; Reis, Veronica Massena

    2009-01-01

    The species Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae and H. rubrisubalbicans are endophytic N2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. However, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in N free semi-solid media which are only semi-selective for the species/genera [the Most Probable Number (MPN) Technique] and each culture must be further subjected to several tests to identify the isolates at the species level. The use of species-specific polyclonal antibodies with the indirect ELISA (enzyme-linked immunosorbent assay) can be an alternative which is rapid and specific to quantify these populations of bacteria. This study was performed to investigate the viability of adapting the indirect ELISA technique to quantify individually the populations of these three species of diazotroph within the root and shoot tissues of sugarcane. The results showed that species-specific polyclonal antibodies could be obtained by purifying sera in protein-A columns which removed non-specific immuno-globulins. It was possible to quantify the three bacterial species in the Brazilian sugarcane variety SP 70-1143 in numbers above 105 cells per g fresh weight in roots, rhizomes and leaves. The numbers of the different bacterial species evaluated using the ELISA technique were found to be higher than when the same populations were evaluated using the MPN technique, reaching 1400 times greater for G. diazotrophicus and 225 times greater for Herbaspirillum spp. These results constitute the first quantification of Herbaspirillum using immunological techniques. PMID:24031435

  14. Relationship between Tree Value, Diameter, and Age in High-Quality Sugar Maple (Acer saccharum) on the Menominee Reservation, Wisconsin

    Science.gov (United States)

    Daniel C. Dey; John Dwyer; Jan Wiedenbeck

    2017-01-01

    Guidelines for managing sugar maple-dominated forests by the single-tree selection method are well established and widely adopted. The forests of the Menominee Tribe in Wisconsin provide an opportunity to validate current guidelines by testing tree value and size/age relationships in forests that have substantially older and larger high-quality trees than can be found...

  15. Spatial relationships between sugar maple (Acer saccharum Marsh), sugar maple decline, slope, aspect, and atmospheric deposition in northern Pennsylvania

    Science.gov (United States)

    Patrick Drohan; Susan Stout; Gary Peterson

    1999-01-01

    Sugar maple decline began to affect Allegheny Plateau forests in the early to mid-1980s. The forests of the region were exposed to several stresses In the period frorn 1985- 1996, including droughts during 1988, 1991, and 1995. Additionally, both native and exotic insects reached epidemic levels during this period (see, for example, Rhoads, 1993). Other documented...

  16. Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies.

    Science.gov (United States)

    da Silva-Froufe, Lúcia Gracinda; Boddey, Robert Michael; Reis, Veronica Massena

    2009-10-01

    The species Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae and H. rubrisubalbicans are endophytic N2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. However, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in N free semi-solid media which are only semi-selective for the species/genera [the Most Probable Number (MPN) Technique] and each culture must be further subjected to several tests to identify the isolates at the species level. The use of species-specific polyclonal antibodies with the indirect ELISA (enzyme-linked immunosorbent assay) can be an alternative which is rapid and specific to quantify these populations of bacteria. This study was performed to investigate the viability of adapting the indirect ELISA technique to quantify individually the populations of these three species of diazotroph within the root and shoot tissues of sugarcane. The results showed that species-specific polyclonal antibodies could be obtained by purifying sera in protein-A columns which removed non-specific immuno-globulins. It was possible to quantify the three bacterial species in the Brazilian sugarcane variety SP 70-1143 in numbers above 10(5) cells per g fresh weight in roots, rhizomes and leaves. The numbers of the different bacterial species evaluated using the ELISA technique were found to be higher than when the same populations were evaluated using the MPN technique, reaching 1400 times greater for G. diazotrophicus and 225 times greater for Herbaspirillum spp. These results constitute the first quantification of Herbaspirillum using immunological techniques.

  17. Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp. Using differente polyclonal antibodies

    Directory of Open Access Journals (Sweden)

    Lúcia Gracinda da Silva-Froufe

    2009-12-01

    Full Text Available The species Gluconacetobacterdiazotrophicus, Herbaspirillum seropedicae and H. rubrisubalbicans are endophytic N2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. However, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in N free semi-solid media which are only semi-selective for the species/genera [the Most Probable Number (MPN Technique] and each culture must be further subjected to several tests to identify the isolates at the species level. The use of species-specific polyclonal antibodies with the indirect ELISA (enzyme-linked immunosorbent assay can be an alternative which is rapid and specific to quantify these populations of bacteria. This study was performed to investigate the viability of adapting the indirect ELISA technique to quantify individually the populations of these three species of diazotroph within the root and shoot tissues of sugarcane. The results showed that species-specific polyclonal antibodies could be obtained by purifying sera in protein-A columns which removed non-specific immuno-globulins. It was possible to quantify the three bacterial species in the Brazilian sugarcane variety SP 70-1143 in numbers above 10(5 cells per g fresh weight in roots, rhizomes and leaves. The numbers of the different bacterial species evaluated using the ELISA technique were found to be higher than when the same populations were evaluated using the MPN technique, reaching 1400 times greater for G. diazotrophicus and 225 times greater for Herbaspirillum spp. These results constitute the first quantification of Herbaspirillum using immunological techniques.

  18. PEMBUATAN BIOETANOL MELALUI FERMENTASI NIRA TEBU (Saccharum officinarum MENGGUNAKAN Saccharomyces cerevisiae DENGAN PENAMBAHAN VITAMIN B KOMPLEKS SEBAGAI NUTRISI FERMENTASI

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    Wahyu Budi Utama

    2016-06-01

    Full Text Available Bioethanol is alternative energy produced from the fermentation of carbohydrates like sugar cane juice. The fermentation process is influenced by several factors like nutrition of fermentation. This research to determine the addition weigh (g of vitamin B complex gr and time of fermentation as theoptimumconditions of fermentaced toproduceethanol with high concentrations. Sugar cane juice as raw material fermented using Saccharomyces cereviceaea with variation additional of vitamin B complex are 0%; 0.1%; 0.2%; and 0.3% (w/v sugar cane juice fermented during time of fermentation with the variation are 6 day, 7 day and 8 day. Purification of bioethanol by process of distillation.Alcohol content analysis using gas chromatography. The results showed optimumconditions of fermentaced are addition of Vitamin B complex is 0.1% (w/v and time of fermentation is 7 days to produced ethanol 30.177%.   Keywords: Ethanol, Fermentation, Sugar cane juice, Vitamin B complex.

  19. Desempenho de trator-implemento na cana-de-açúcar em sistemas de plantio direto e convencional Tractor-implement performance in sugarcane under no-tillage and conventional planting systems

    Directory of Open Access Journals (Sweden)

    José B. Duarte Júnior

    2008-12-01

    Full Text Available Com este trabalho objetivou-se avaliar o desempenho de conjuntos mecanizados, através da patinagem, da velocidade do trator e da viabilidade de operação de plantio da cana-de-açúcar (Saccharum sp. em sistema de plantio direto (SPD e convencional (PC sobre diferentes plantas de cobertura. O delineamento experimental utilizado foi o inteiramente casualizado (DIC, com três repetições, em esquema de faixas. Constituíram-se 24 tratamentos pelas combinações de parcelas: A1-PD da cana sobre crotalária; a²-PD da cana sobre feijão de porco; A3-PC da cana sobre mucuna preta incorporada; A4-PC da cana (testemunha; de subparcelas: B1-FORD 8430 DT + plantadora de duas linhas; B2-FORD 8430 DT + sulcador de três linhas; B3-FORD 5610 + sulcador de duas linhas; e de sub-subparcelas: C1 - 1ª marcha reduzida e C2 - 2ª marcha reduzida. Observou-se que as operações de sulcagem para posterior plantio manual ou sulcagem e plantio mecanizado da cana-de-açúcar em SPD sobre as plantas de cobertura sem serem manejadas anteriormente com roçadora ou rolo-faca, foram possíveis e igualmente eficientes quando comparadas ao PC, portanto, é possível reduzir o tempo de implantação da cultura e os custos operacionais devido à diminuição do número de operações no SPD da cana-de-açúcar.This research evaluated the performance of automated groups using slippage, tractor speed, and operational viability of planting sugarcane (Saccharum spp. in no-tillage (SPD and conventional (PC systems under different plant coverings. The experimental design was completely randomized, using three repetitions in a split-split plot. There were twenty four treatments: A1-SPD the sugarcane on sunnhemp (Crotalaria juncea; a²-SPD the sugarcane on the jack bean (Canavalia ensiformis; A3-PC the sugarcane on incorporated black mucuna (Stilozobium aterrimum; A4-PC the sugarcane (control; sub-plots: B1-FORD 8430 DT + two row crop planter; B2-FORD 8430 DT + three lines furrow

  20. Reguladores vegetais e qualidade tecnológica da cana-de-açúcar em meio de safra Growth regulators and technological quality of sugarcane in the middle of the cropping season

    Directory of Open Access Journals (Sweden)

    Glauber Henrique Pereira Leite

    2008-12-01

    Full Text Available A maturação da cana-de-açúcar (Saccharum officinarum L. refere-se a um dos aspectos fundamentais para o sistema de produção da indústria sucroalcooleira. Objetivou-se, neste trabalho, avaliar a qualidade tecnológica da cana-de-açúcar em função da aplicação de reguladores vegetais em meio de safra. O delineamento experimental utilizado foi o de blocos casualizados com cinco repetições, sendo os tratamentos constituídos da aplicação de quatro maturadores da classe dos retardantes de crescimento (Ethefon, Etil-trinexapac, KNO3 e KNO3 + Boro e uma testemunha (maturação natural. A eficiência agronômica dos maturadores foi influenciada pela época de aplicação, pela condição climática e pela característica genética da variedade. A aplicação dos maturadores em 10/05/2004 permitiu explorar de forma significativa o potencial genético da variedade quanto ao acúmulo de sacarose nos colmos, implicando em melhoria na qualidade tecnológica da matéria-prima. A adoção dessa prática em 04/08/2005, de modo geral, pouco afetou os parâmetros tecnológicos, exceto a aplicação de Etil-trinexapac, o qual induziu melhoria significativa na qualidade da matéria-prima. A produtividade de açúcar refletiu-se de forma direta na margem de contribuição agrícola, sendo constatado maior retorno econômico para as plantas tratadas com KNO3 na safra 2004.The ripening of sugarcane (Saccharum officinarum L. is the most important aspect in the production system for sugar and alcohol industry. The objective of this work was to evaluate the technological quality of sugarcane as a function of plant regulators application in the middle of the cropping season. The experiment was carried out in a randomized block design with five replications. The treatments consisted of four plant regulators application of the class of growth retainers (Ethephon, Ethyl.trinexapac, Potassium nitrate and Potassium nitrate + Boron and a control (natural

  1. Composição química da cana-de-açucar (Saccharum SPP. E das silagens com diferentes aditivos em duas idades de corte Chemical composition of sugar cane (Saccharum SPP. and of the silages with different additives at two cutting ages

    Directory of Open Access Journals (Sweden)

    Roberto Valadares Santos

    2006-12-01

    Full Text Available Objetivou-se com este estudo avaliar a composição química da forragem e da silagem de cana-de-açúcar em duas idades de corte com diferentes aditivos. Os tratamentos foram arranjados em um delineamento inteiramente casualizado, com três repetições, em esquema fatorial 2 x 9, sendo duas idades de corte (11 e 24 meses e nove formas de uso (cana picada acrescida de 1% da mistura uréia e sulfato de amônio, cana in natura e ensilada sem aditivo, cana in natura e ensilada com 1% de uréia, cana in natura e ensilada com de 8% de MDPS e cana in natura e ensilada com de 0,5% de sal mineral. A concentração de MS da cana-de-açúcar nos 11 meses foi em média , 25,9% inferior a de 24 meses de idade. Os valores de pH não variaram com as idades de corte, porém, diferiram entre os tratamentos silagem e cana in natura. A cana in natura adicionada com 1% da mistura uréia + sulfato de amônio resultou nos melhores valores bromatológicos, o mesmo ocorrendo com a cana ensilada com uréia, sendo portanto mais conveniente ensilá-la do que deixá-la no campo por mais um período de cultivo. O material ensilado apresentou teores mais elevados de PB, FDN e FDA.It was aimed by this study to evaluate the chemical composition of the sugar cane forage and silage at two cutting ages with different additives. The treatments were arranged in a completely randomized design with three replicates in a 2 x 9 factorial scheme, their being two cutting ages (11 and 24 months and nine forms of use (chopped cane added of 1% of the mixture urea and ammonium sulfate, in natura cane and ensiled without any additive, in natura cane and ensiled with 1% of urea, in natura cane and ensiled with 8% of GEH (ground ears with husks and in natura cane and ensiled with 0.5% of mineral salt. The concentration of DM of the sugar cane of the 11 months was, on average, 25.9% inferior to that at 24 months of age. The pH values did not range with the cutting ages but they differed between the treatments silage and in natura cane. The in natura cane added with 1% of the mixture urea + ammonium sulfate resulted into the best bromatologic values, the same occurring with the cane ensiled with urea, it being ,therefore, more convenient to ensile it than to leave it in the field for a further cultivation period. The ensiled material showed more elevated contents of CP, NDF and ADF.

  2. Pemanfaatan Vinasse -Limbah Industri Alkohol- untuk Perbaikan Sifat Fisik Tanah dalam Pengembangan Tebu (Saccharum officinarum L di Lahan Pasir Pantai

    Directory of Open Access Journals (Sweden)

    M. Zulfan Arrodli

    2016-05-01

    Full Text Available Lahan pasir pantai merupakan lahan bermasalah untuk pertanian karena sifat tanahnya tidak mendukung pertumbuhan tanaman dan vinasse merupakan limbah industri ethanol yang jumlahnya sangat besar yang apabila dibuang di lahan terbuka akan mencemari lingkungan. Penggunaan vinasse sebagai bahan perbaikan tanah tanpa memberikan pengaruh negatif kemungkinan dapat dikerjakan untuk tanah bertekstur kasar seperti tanah di lahan pasir pantai. Oleh karena itu dalam penelitian ini, vinasse akan dicoba digunakan untuk membudidayakan tebu pada media tanah yang diambil dari lahan pasir pantai. Penelitian menggunakan pendekatan percobaan pot faktorial 4x4 yang disusun dalam rancangan lingkungan acak lengkap dengan 3 ulangan. Hasil penelitian menunjukkan bahwa pemberian vinasse dengan dosis 60.000 l/ha hanya sekali pada awal penanaman tebu mampu memperbaiki kemampuan tanah menyimpan air. Vinasse terbukti tidak memberikan pengaruh kurang baik terhadap tanaman yang dibudidayakan, bahkan pemberian vinasse cenderung memperbaiki pertumbuhan tebu baik dilihat pada tinggi tanaman, diameter batang, berat kering akar dan tajuk, meskipun pengaruhnya tidak berbeda nyata.

  3. Antioxidant activity, inhibition of nitric oxide overproduction, and in vitro antiproliferative effect of maple sap and syrup from Acer saccharum.

    Science.gov (United States)

    Legault, Jean; Girard-Lalancette, Karl; Grenon, Carole; Dussault, Catherine; Pichette, André

    2010-04-01

    Antioxidant activity, inhibition of nitric oxide (NO) overproduction, and antiproliferative effect of ethyl acetate extracts of maple sap and syrup from 30 producers were evaluated in regard to the period of harvest in three different regions of Québec, Canada. Oxygen radical absorbance capacity (ORAC) values of maple sap and syrup extracts are, respectively, 12 +/- 6 and 15 +/- 5 micromol of Trolox equivalents (TE)/mg. The antioxidant activity was also confirmed by a cell-based assay. The period of harvest has no statistically significant incidence on the antioxidant activity of both extracts. The antioxidant activity of pure maple syrup was also determined using the ORAC assay. Results indicate that the ORAC value of pure maple syrup (8 +/- 2 micromol of TE/mL) is lower than the ORAC value of blueberry juice (24 +/- 1 micromol of TE/mL) but comparable to the ORAC values of strawberry (10.7 +/- 0.4 micromol of TE/mL) and orange (10.8 +/- 0.5 micromol of TE/mL) juices. Maple sap and syrup extracts showed to significantly inhibit lipopolysaccharide-induced NO overproduction in RAW264.7 murine macrophages. Maple syrup extract was significantly more active than maple sap extract, suggesting that the transformation of maple sap into syrup increases NO inhibition activity. The highest NO inhibition induced by the maple syrup extracts was observed at the end of the season. Moreover, darker maple syrup was found to be more active than clear maple syrup, suggesting that some colored oxidized compounds could be responsible in part for the activity. Finally, maple syrup extracts (50% inhibitory concentration = 42 +/- 6 microg/mL) and pure maple syrup possess a selective in vitro antiproliferative activity against cancer cells.

  4. Phytochemical profile of sugarcane and its potential health aspects.

    Science.gov (United States)

    Singh, Amandeep; Lal, Uma Ranjan; Mukhtar, Hayat Muhammad; Singh, Prabh Simran; Shah, Gagan; Dhawan, Ravi Kumar

    2015-01-01

    Sugarcane (Saccharum officinarum Linn.) is an important perennial grass of Poaceae family, indigenous to tropical South Asia and Southeast Asia. It is cultivated worldwide due to the economical and medicinal value of its high yielding products. Sugarcane juice is well known as a raw material for the production of refined sugar and its wax is considered as a potential substitute for the expensive carnauba wax, which is of cosmetic and pharmaceutical interest. Refined sugar is the primary product of sugarcane juice, but during its processing, various other valuable products are also obtained in an unrefined form, such as, brown sugar, molasses, and jaggery. Sugarcane juice is widely used in India in the treatment of jaundice, hemorrhage, dysuria, anuria, and other urinary diseases. Herein, we have summarized the different phytoconstituents and health benefits of sugarcane and its valuable products. The phytochemistry of sugarcane wax (obtained from the leaves and stalks of sugarcane), leaves, juice, and its products has revealed the presence of various fatty acid, alcohol, phytosterols, higher terpenoids, flavonoids, -O- and -C-glycosides, and phenolic acids. The future prospective of some of the sugarcane products has been discussed, which needs a phytopharmacological study and has a great potential to be a valuable medicinal product.

  5. Penerapan Produksi Bersih Dalam Upaya Penurunan Timbulan Limbah Cair Di Pabrik Gula Tebu

    Directory of Open Access Journals (Sweden)

    Yudith Vega Paramitadevi

    2017-11-01

    Full Text Available Tebu (Saccharum officianarum L. adalah tanaman yang mempunyai banyak manfaat, salah satunya sukrosa yang terdapat pada batang tebu yang dimanfaatkan untuk menghasilkan kristal gula. Kristal gula yang terbentuk mengalami pemrosesan yang panjang, dimulai dari penggilingan tebu hingga pemasakan. Pada proses pengolahan tersebut akan menghasilkan gula produk sekaligus limbah cair yang memiliki beban pencemaran organik tinggi. Tindak produksi bersih dalam industri gula tebu diperlukan agar penghematan air baku dan pencemaran air dapat terlaksana. Kajian terhadap produksi bersih dilakukan melalui studi kasus yang sifatnya deskriptif pada salah satu industri gula tebu di Indonesia. Identifikasi terhadap peluang produksi bersih dilakukan agar diperoleh pengaruh keseluruhan terhadap kinerja lingkungan, terutama pengurangan terhadap beban influen yang masuk ke IPAL. Sebanyak 6 alternatif peluang produksi bersih untuk penghematan konsumsi air baku dan 4 alternatif peluang produksi bersih untuk reduksi timbulan limbah cair diberikan. Peluang produksi bersih yang akhirnya dapat diterapkan yakni recycle air kondensat larutan gula, air bekas scrubber dan penerapan good house keeping. Debit air yang masuk ke IPAL berkurang 51.72%, setara dengan 4500 m3 air baku per hari, atau penghematan sebesar Rp 17.235.310,00 per tahun. Perancangan desain IPAL ulang juga diusulkan untuk mengatasi permasalahan konvensional yang melekat pada sistem kolam anaerobik-aerobik.

  6. Effect of Organic Amendments and Chemical Fertilization in Production of Corn (Zea Mays L.

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    Fabio Emilio Forero Ulloa

    2014-11-01

    Full Text Available Corn is grown in 135 countries, and because of its uses and nutritional benefits is the world's most important cereal. In Colombia it is grown in various agro-ecological conditions of production. The bagasse is an organic residue resulting from the grinding of sugar cane (Saccharum officinarum L., used for the production of jaggery (solid resulting of boiling and evaporation of the juice from sugar cane, which can be used as an amendment and is a soil conditioner, as a rich source of phosphorus, calcium and nitrogen. The aim of the research was to evaluate the effect of bagasse against the application of other organic sources and chemical fertilization in maize, variety ICA-V-305. For this, a completely random statistical design with four treatments and absolute control was established. Results were subjected to analysis of variance and Tukey comparison test. Applying Bagasse + Abimgra® produced the greatest number of ears of corn, while the use of only bagasse, presented the second best results in terms of number of grains / ear and weight of 100 grains of corn, therefore bagasse becomes , through time, an important option as organic amendment, which would favor the production of corn, and an option as organic fertilizer.

  7. Effect of agitation rate on ethanol production from sugar maple hemicellulosic hydrolysate by Pichia stipitis.

    Science.gov (United States)

    Shupe, Alan M; Liu, Shijie

    2012-09-01

    Concentrated dilute acid hydrolysate was obtained from hot water extracts of Acer saccharum (sugar maple) and was fermented to ethanol by Pichia stipitis in a 1.3-L-benchtop bioreactor. The conditions under which the highest ethanol yield was achieved were when the air flow rate was set to 100 cm(3) and the agitation rate was set to 150 rpm resulting in an overall mass transfer coefficient (K(L)a) of 0.108 min(-1). A maximum ethanol concentration of 29.7 g/L was achieved after 120 h of fermentation; however, after 90 h of fermentation, the ethanol concentration was only slightly lower at 29.1 g/L with a yield of 0.39 g ethanol per gram of sugar consumed. Using the same air flow rate and adjusting the agitation rate resulted in lower ethanol yields of 0.25 g/g at 50 rpm and 0.30 g/g at 300 rpm. The time it takes to reach the maximum ethanol concentration was also affected by the agitation rate. The ethanol concentration continued to increase even after 130 h of fermentation when the agitation rate was set at 50 rpm, whereas the maximum ethanol concentration was reached after only 68.5 h at 300 rpm.

  8. Remote sensing of changes in morphology and physiology of trees under stress

    Science.gov (United States)

    Olson, C. E., Jr.; Rohde, W. G.; Ward, J. M.

    1970-01-01

    Results of continuing studies of forest trees subjected to varying types of stress are reported. Both greenhouse and field studies are included. Greenhouse work with tree seedlings exposed to varying levels of NaCl and CaCl2 in the soil indicated that, in the initial stages, palisade cells shrink and the amount of air space in the leaf increases. As the severity of damage increases, the cells of the spongy mesophyll shrink and flatten, and the amount of air space in the leaf decreases. Statistical analysis of foliar reflectance and associated moisture content data led to a series of regression equations for predicting foliar moisture content from reflectance data. Equations were calculated for three species, yellow birch (Betula alleghaniensis Britton), sugar maple (Acer saccharum Marsh.) and white ash (Fraxinus americana L.) having multiple correlation coefficients of 0.98, 0.94 and 0.93 respectively. Interpretation of multispectral imagery of the Ann Arbor Forestry Test Site (NASA Site 190) provided evidence that infections of Fomes annosus can be detected in the early stages. Infections of two needle cast diseases were also detected in conifer plantations in the test site. A study of automatic interpretation of multispectral scanner imagery for tree species recognition provided encouraging results.

  9. Nitrogen derived from fertilization and straw for plant cane nutrition

    International Nuclear Information System (INIS)

    Vitti, Andre Cesar; Faroni, Carlos Eduardo

    2011-01-01

    The objective of this work was to evaluate the recovery, by plant cane, of the nitrogen ( 15 N) from urea and from sugarcane (Saccharum spp.) crop residues - straw and root system - incorporated into the soil. The experiment was settled in 2005/2006 with the sugarcane cultivar SP81 3250. At planting, microplots of 2 m length and 1.5 m width were installed, and N applications were done with 80 kg ha-1 N (urea with 5.05% in 15 N atoms) and 14 Mg ha -1 crop residues - 9 Mg ha -1 of sugarcane straw and 5 Mg ha -1 of root system, labeled with 15 N (1.07 and 0.81% in 15 N atoms, respectively). The total N accumulation by plants was determined during the crop cycle. Although the N use by shoot from crop residue mineralization (PA and SR) increased significantly over time, this source hardly contributed to crop nutrition. The recovery of the 15 N-urea, 15 N-SS and 15 N-RS by plant cane was 30.3 +- 3.7%, 13.9 +- 4.5% and 6.4 +- 0.9%, respectively, representing 15.9, 4.7 and 1.4% of total nitrogen uptake by shoot. (author)

  10. Volatile organic emissions from the distillation and pyrolysis of vegetation

    Directory of Open Access Journals (Sweden)

    J. P. Greenberg

    2006-01-01

    Full Text Available Leaf and woody plant tissue (Pinus ponderosa, Eucalyptus saligna, Quercus gambelli, Saccharum officinarum and Oriza sativa were heated from 30 to 300°C and volatile organic compound (VOC emissions were identified and quantified. Major VOC emissions were mostly oxygenated and included acetic acid, furylaldehyde, acetol, pyrazine, terpenes, 2,3-butadione, phenol and methanol, as well as smaller emissions of furan, acetone, acetaldehyde, acetonitrile and benzaldehyde. Total VOC emissions from distillation and pyrolysis were on the order of 10 gC/kgC dry weight of vegetation, as much as 33% and 44% of CO2 emissions (gC(VOC/gC(CO2 measured during the same experiments, in air and nitrogen atmospheres, respectively. The emissions are similar in identity and quantity to those from smoldering combustion of woody tissue and of different character than those evolved during flaming combustion. VOC emissions from the distillation of pools and endothermic pyrolysis under low turbulence conditions may produce flammable concentrations near leaves and may facilitate the propagation of wildfires. VOC emissions from charcoal production are also related to distillation and pyrolysis; the emissions of the highly reactive VOCs from production are as large as the carbon monoxide emissions.

  11. Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

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    Duli Zhao

    2015-07-01

    Full Text Available Sugarcane (Saccharum spp. brown rust (caused by Puccinia melanocephala Syd. & P. Syd. was first reported in the United States in 1978 and is still one of the great challenges for sugarcane production. A better understanding of sugarcane genotypic variation in response to brown rust will help optimize breeding and selection strategies for disease resistance. Brown rust ratings were scaled from non-infection (0 to severe infection (4 with intervals of 0.5 and routinely recorded for genotypes in the first clonal selection stage of the Canal Point sugarcane breeding program in Florida. Data were collected from 14,272 and 12,661 genotypes and replicated check cultivars in 2012 and 2013, respectively. Mean rust rating, % infection, and severity in each family and progeny of female parent were determined, and their coefficients of variation (CV within and among families (females were estimated. Considerable variation exists in rust ratings among families or females. The families and female parents with high susceptibility or resistance to brown rust were identified and ranked. The findings of this study can help scientists to evaluate sugarcane crosses and parents for brown rust disease, to use desirable parents for crossing, and to improve genetic resistance to brown rust in breeding programs.

  12. Relationship between sugarcane rust severity and soil properties in louisiana.

    Science.gov (United States)

    Johnson, Richard M; Grisham, Michael P; Richard, Edward P

    2007-06-01

    ABSTRACT The extent of spatial and temporal variability of sugarcane rust (Puccinia melanocephala) infestation was related to variation in soil properties in five commercial fields of sugarcane (interspecific hybrids of Saccharum spp., cv. LCP 85-384) in southern Louisiana. Sugarcane fields were grid-soil sampled at several intensities and rust ratings were collected at each point over 6 to 7 weeks. Soil properties exhibited significant variability (coefficients of variation = 9 to 70.1%) and were spatially correlated in 39 of 40 cases with a range of spatial correlation varying from 39 to 201 m. Rust ratings were spatially correlated in 32 of 33 cases, with a range varying from 29 to 241 m. Rust ratings were correlated with several soil properties, most notably soil phosphorus (r = 0.40 to 0.81) and soil sulfur (r = 0.36 to 0.68). Multiple linear regression analysis resulted in coefficients of determination that ranged from 0.22 to 0.73, and discriminant analysis further improved the overall predictive ability of rust models. Finally, contour plots of soil properties and rust levels clearly suggested a link between these two parameters. These combined data suggest that sugarcane growers that apply fertilizer in excess of plant requirements will increase the incidence and severity of rust infestations in their fields.

  13. Acclimation and soil moisture constrain sugar maple root respiration in experimentally warmed soil.

    Science.gov (United States)

    Jarvi, Mickey P; Burton, Andrew J

    2013-09-01

    The response of root respiration to warmer soil can affect ecosystem carbon (C) allocation and the strength of positive feedbacks between climatic warming and soil CO2 efflux. This study sought to determine whether fine-root (maple (Acer saccharum Marsh.)-dominated northern hardwood forest would adjust to experimentally warmed soil, reducing C return to the atmosphere at the ecosystem scale to levels lower than that would be expected using an exponential temperature response function. Infrared heating lamps were used to warm the soil (+4 to +5 °C) in a mature sugar maple forest in a fully factorial design, including water additions used to offset the effects of warming-induced dry soil. Fine-root-specific respiration rates, root biomass, root nitrogen (N) concentration, soil temperature and soil moisture were measured from 2009 to 2011, with experimental treatments conducted from late 2010 to 2011. Partial acclimation of fine-root respiration to soil warming occurred, with soil moisture deficit further constraining specific respiration rates in heated plots. Fine-root biomass and N concentration remained unchanged. Over the 2011 growing season, ecosystem root respiration was not significantly greater in warmed soil. This result would not be predicted by models that allow respiration to increase exponentially with temperature and do not directly reduce root respiration in drier soil.

  14. Fire History of Appalachian Forests of the Lower St-Lawrence Region (Southern Quebec

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    Serge Payette

    2017-04-01

    Full Text Available Sugar maple (Acer saccharum forests are among the main forest types of eastern North America. Sugar maple stands growing on Appalachian soils of the Lower St-Lawrence region are located at the northeastern limit of the northern hardwood forest zone. Given the biogeographical position of these forests at the edge of the boreal biome, we aimed to reconstruct the fire history and document the occurrence of temperate and boreal trees in sugar maple sites during the Holocene based on soil macrocharcoal analysis. Despite having experienced a different number of fire events, the fire history of the maple sites was broadly similar, with two main periods of fire activity, i.e., early- to mid-Holocene and late-Holocene. A long fire-free interval of at least 3500 years separated the two periods from the mid-Holocene to 2000 years ago. The maple sites differ with respect to fire frequency and synchronicity of the last millennia. According to the botanical composition of charcoal, forest vegetation remained relatively homogenous during the Holocene, except recently. Conifer and broadleaf species coexisted in mixed forests during the Holocene, in phase with fire events promoting the regeneration of boreal and temperate tree assemblages including balsam fir (Abies balsamea and sugar maple.

  15. Adenylate control contributes to thermal acclimation of sugar maple fine-root respiration in experimentally warmed soil.

    Science.gov (United States)

    Jarvi, Mickey P; Burton, Andrew J

    2018-03-01

    We investigated the occurrence of and mechanisms responsible for acclimation of fine-root respiration of mature sugar maple (Acer saccharum) after 3+ years of experimental soil warming (+4 to 5 °C) in a factorial combination with soil moisture addition. Potential mechanisms for thermal respiratory acclimation included changes in enzymatic capacity, as indicated by root N concentration; substrate limitation, assessed by examining nonstructural carbohydrates and effects of exogenous sugar additions; and adenylate control, examined as responses of root respiration to a respiratory uncoupling agent. Partial acclimation of fine-root respiration occurred in response to soil warming, causing specific root respiration to increase to a much lesser degree (14% to 26%) than would be expected for a 4 to 5 °C temperature increase (approximately 55%). Acclimation was greatest when ambient soil temperature was warmer or soil moisture availability was low. We found no evidence that enzyme or substrate limitation caused acclimation but did find evidence supporting adenylate control. The uncoupling agent caused a 1.4 times greater stimulation of respiration in roots from warmed soil. Sugar maple fine-root respiration in warmed soil was at least partially constrained by adenylate use, helping constrain respiration to that needed to support work being performed by the roots. © 2017 John Wiley & Sons Ltd.

  16. Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

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    Renata Fava Ditt

    2011-08-01

    Full Text Available Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp. is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC. With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

  17. Potential of antioxidant and toxicity of some medical plants used by sub-ethnic communities of Bahau in East Kalimantan

    Science.gov (United States)

    Rohim, P.; Arung, E. T.; Kusuma, I. W.

    2018-04-01

    The purpose of this research is to assay the potential antioxidant and toxicity of several plants from Bahau, a sub-ethnic in East Kalimantan in regard to their utilization as traditional medicines. This research includes phytochemical analysis, DPPH radical and superoxide radical scavenging activity as well as toxicity assay using Artemiasalina shrimp larvae. The results of the extraction showed the highest yield was 2,91% obtained from avung tanaq (Ficus uncinata), while the lowest is 1.14% obtained from tevoqsalah (Saccharum sp.) species. The result of phytochemicals showed that all plants contain alkaloid and carbohydrate. While carotenoids, saponins, triterpenoids and steroids were absence in all plant extracts. The DPPH radical scavenging activity test showed that the lowest IC50 value of kayog kue (Dictamnus albus) by 23.96 μg/mL. The superoxide radical scavenging activity assay showed IC50 values of all extract samples were >100 μg/mL. The toxicity assay showed that LC50 values of all samples of extract tested were >1000 μg/mL. The present research suggested good potential activity of some plants from Bahau ethnic and further research oriented to wide uses of the plants as herbal products is needed.

  18. Selected Abiotic and Biotic Environmental Stress Factors Affecting Two Economically Important Sugarcane Stalk Boring Pests in the United States

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    Allan T. Showler

    2016-02-01

    Full Text Available Sugarcane, Saccharum spp., in the United States is attacked by a number of different arthropod pests. The most serious among those pests are two stalk boring moths in the Family Crambidae: the sugarcane borer, Diatraea saccharalis (F., and the Mexican rice borer, Eoreuma loftini (Dyar. The two species are affected by abiotic and biotic environmental stress factors. Water deficit and excessive soil nitrogen alter physical and physiochemical aspects of the sugarcane plant that make the crop increasingly vulnerable to E. loftini. Weed growth can be competitive with sugarcane but it also supports enhanced abundances and diversity of natural enemies that can suppress infestations of D. saccharalis. In an instance where the stalk borer is considered a stress factor, proximity of vulnerable crops to sugarcane can influence levels of E. loftini infestation of sugarcane. The adverse effects of each stress factor, in terms of stalk borer attack, can be reduced by adopting appropriate cultural practices, such as adequate irrigation, judicious use of nitrogen fertilizer, using noncompetitive weed growth, and not planting vulnerable crops near sugarcane fields. Understanding the relationships between stress factors and crop pests can provide valuable insights for plant breeders and tools for incorporation into integrated pest management strategies.

  19. Descriptions and Seasonal Variations of Various Biotopes and Ecotones of Indus River Bed at Chashma Barrage, Pakistan

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    Tahira Hussain

    2015-01-01

    Full Text Available The aim of the study was to find the diversity index of flora, investigation of species at pond area and biomass calculation of economic plants at Chashma Barrage, Pakistan. The study area consisted of river Bella, pond area and eastern edge of river bed near Ali Wali Ghandi village at Chashma Barrage. The seasonal variation in the abundance of floral species was also related with the increase in temperature. Cyperus deformus, Phragmites kerka, Saccharum spontaneum were most abundant in the river ‘Bella’ habitat. Persicassia amphibian, Cyperus deformus and Polygonum royleanum were abundant in Aliwali Ghandi habitat. Aquatic plants of families Potamogetonaceae, Nymphaeaceae, Najadaceae and Hydrocharitaceae were most common in pond area . The ecotones were wide and variable between the aquatic biotopes and the river bed biotopes. On the Aliwali Ghandi site the moist soil with puddles and small channels of water were found and Cyperus deformus biotope prevailed. In the river ‘Bella’ site Phragmites, Sacharum was the biotope and in water Hydrilla biotope was recorded. An increase in biomass of Phragmites, Sacharum and Typha was recorded with the passage of time. The species of the area have significant importance in socio economics of the local community of Chashma Barrage.

  20. Nitrogen derived from fertilization and straw for plant cane nutrition; Nitrogenio proveniente da adubacao nitrogenada e de residuos culturais na nutricao da cana-planta

    Energy Technology Data Exchange (ETDEWEB)

    Vitti, Andre Cesar, E-mail: acvitti@apta.sp.gov.b [Agencia Paulista de Tecnologia dos Agronegocios (APTA), Piracicaba, SP (Brazil). Polo Regional Centro-Sul; Franco, Henrique Coutinho Junqueira, E-mail: henrique.franco@bioetanol.org.b [Laboratorio Nacional de Ciencia e Tecnologia do Bioetanol (CTBE), Campinas, SP (Brazil). Centro de Pesquisa em Energia e Materiais; Trivelin, Paulo Cesar Ocheuze; Ferreira, Danilo Alves; Otto, Rafael; Fortes, Caio, E-mail: pcotrive@cena.usp.b, E-mail: danilo.alves.ferreira@usp.b, E-mail: rotto@esalq.usp.b, E-mail: cfortes@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Faroni, Carlos Eduardo, E-mail: cfaroni@ctc.com.b [Centro de Tecnologia Canavieira, Piracicaba, SP (Brazil)

    2011-07-01

    The objective of this work was to evaluate the recovery, by plant cane, of the nitrogen ({sup 15}N) from urea and from sugarcane (Saccharum spp.) crop residues - straw and root system - incorporated into the soil. The experiment was settled in 2005/2006 with the sugarcane cultivar SP81 3250. At planting, microplots of 2 m length and 1.5 m width were installed, and N applications were done with 80 kg ha-1 N (urea with 5.05% in {sup 15}N atoms) and 14 Mg ha{sup -1} crop residues - 9 Mg ha{sup -1} of sugarcane straw and 5 Mg ha{sup -1} of root system, labeled with {sup 15}N (1.07 and 0.81% in {sup 15}N atoms, respectively). The total N accumulation by plants was determined during the crop cycle. Although the N use by shoot from crop residue mineralization (PA and SR) increased significantly over time, this source hardly contributed to crop nutrition. The recovery of the {sup 15}N-urea, {sup 15}N-SS and {sup 15}N-RS by plant cane was 30.3 +- 3.7%, 13.9 +- 4.5% and 6.4 +- 0.9%, respectively, representing 15.9, 4.7 and 1.4% of total nitrogen uptake by shoot. (author)

  1. SPECIES COMPOSITION, DISTRIBUTION, LIFE FORMS AND FOLK NOMENCLATURE OF FOREST AND COMMON LAND PLANTS OF WESTERN CHITWAN, NEPAL

    Science.gov (United States)

    Dangol, D. R.

    2012-01-01

    This paper enumerates 349 plant species belonging to 77 families of vascular plants collected in the winter seasons of 1996 and 2000 by the flora teams of the Population and Ecology Research Laboratory, Nepal. Of the total species, 249 species belong to dicotyledons, 87 species to monocotyledons and 13 species to pteridophytes. Among the families, dicotyledons contributed the highest number of families (55 in number) followed by monocotyledons and pteridophytes. In the study areas, species composition varies with the type of habitats in the study plots. Some species are unique in distribution. The highest unique species are contributed by common lands (87 spp.), followed by the Chitwan National Park forest (36 spp.) and Tikauli forest (32 spp.). Ageratum houstonianum Mill., Cynodon dactylon (L.) Pers., Imperata cylindrica (L.) Beauv., Rungia parviflora (Retz.) Nees, Saccharum spontaneum L. and Thelypteris auriculata (J. Sm.) K. Iwats are the most common species across all the research blocks. Of the listed plants, many plants have local names either in Nepalese or other tribal languages. Plants are named in different ways on the basis of habit, habitat, smell, taste, and morphological characters of the plants, which are also the basis of nomenclature in plant taxonomy. PMID:22962539

  2. Selectividad de caña de azúcar en bovinos

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    E. M. Aranda

    2009-01-01

    Full Text Available Se condujo este trabajo con el objetivo de conocer la aceptación del forraje de caña de azúcar integral en la dieta con base de pasto para bovinos adultos fistulados en rumen y duodeno estabulados; para ello, se ofreció pasto estrella africana (Cynodon plectostachyus y caña de azúcar (Saccharum officinarum mezclada con 1% de urea a voluntad, por separado. Se midió la composición de los alimentos, consumo de materia seca, pH ruminal y duodenal. Se utilizaron técnicas de la estadística descriptiva, como la media aritmética y error estándar. La proteína, paredes celulares y hemicelulosa fueron mayores para el pasto estrella. El consumo de MS fue de 46.6 y 38.1% para la caña de azúcar y el pasto, respectivamente; el pH ruminal tuvo valores de 7.3, disminuyendo a 6.7 a las 12 h.; y el pH de la digesta duodenal osciló de 3.7 a 4.7. Se concluye que hubo mayor aceptación por la caña de azúcar y el pH ruminal no indica valores que afecten la celulólisis ruminal.

  3. AISLAMIENTO Y CONTROL MICROBIOLÓGICO DE Leuconostoc mesenteroides, EN UN INGENIO PARA OPTIMIZAR EL RENDIMIENTO DE AZÚCAR Y ETANOL ISOLAMENTO E Leuconostoc Mesenteroides CONTROLE MICROBIOLÓGICO EM UM TALENTO PARA OTIMIZAR O DESEMPENHO DE AÇÚCAR E ETANOL ISOLATION AND MICROBIOLOGICAL CONTROL OF Leuconostoc mesenteroides, IN TO SUGAR REFINERY TO OPTIMIZE THE PERFORMANCE OF SUGAR AND ETHANOL

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    RAÚL A CUERVO MULET

    2010-12-01

    Full Text Available La caña de azúcar (Saccharum officinarum L es exprimida para obtener su jugo que después de ser purificado y neutralizado, deja cristalizar el azúcar. Considerando la importancia que representa la caña de azúcar para la industria azucarera, se hace necesario incrementar el contenido de sacarosa libre de dextrana (destrucción de sacarosa causada por la acción de microorganismos acompañantes de la caña, siendo uno de los más importantes Leuconostoc mesenteroides. En este trabajo de investigación se determinó la inhibición de la bacteria ácido-láctica L mesenteroides aislada en un ingenio azucarero del Valle del Cauca mediante la utilización de microorganismos antagonistas. Para esto, se realizaron aislamientos y cultivos de L. mesenteroides provenientes del jugo de la caña y se realizaron las diferentes pruebas bioquímicas y microbiológicas en el laboratorio para el aislamiento y la identificación de las cepas tanto de Leuconostoc mesenteroides, como las cepas bacterianas antagonistas a ella. Se comprobó que las cepas de los hongos Metharhizium anisopliae y Trichoderma sp, fueron las más eficientes en el control de crecimiento de L mesenteroides, mostrando significativamente un mayor porcentaje de inhibición en comparación de los hongos filamentosos Colletotrichum sp, Thichoderma viridae, Rhizoctonia sp y las bacterias Bacillus subtilis y Serratia marcenses y las levaduras Rhodothorula Rubra y Kloeckera japónica, no presentaron inhibición del crecimiento de Lmesenteroides. Además, se identificó a los hongos Aspergillus sp, Botrytis sp y Streptomyces sp como antagonistas naturales y habituales presentes en el jugo de caña de los Ingenios.Cana (Saccharum officinarum L é comprimida para que seu suco depois de ser purificada e neutralizado, permitiu a cristalizar o açúcar. Considerando a importancia de representar a cana para a indústria de açúcar é necessária para melhorar o conteúdo de sacarose, dextrana livre

  4. Estudo etnobotânico na comunidade de Conceição-Açu (alto da bacia do rio Aricá Açu, MT, Brasil Ethnobotany study in community of Conceição-Açu (on the upper basin of the River Aricá Açu, MT, Brazil

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    Maria Corette Pasa

    2005-06-01

    Full Text Available O presente trabalho tem como objetivo realizar o levantamento etnobotânico de plantas classificadas em diferentes categorias de uso na comunidade de Conceição-Açu, no município de Cuiabá, MT, Brasil e estimar o valor de uso das espécies botânicas em matas de galeria. Entrevistas estruturadas e semi-estruturadas, o uso do questionário foram aplicados em 59 residentes adultos de ambos os sexos. Identificou-se as unidades de paisagem: quintais, roças e matas de galeria e o número total de espécies utilizadas foi de 180. A maioria das plantas foi coletada durante a entrevista e depositadas no Herbário da UFMT. Nos quintais das residências identificou-se 86 espécies, pertencentes a 43 famílias, a maioria cultivada e utilizada como alimento (48,1% e como remédio (44,5%. Nas roças os principais cultivos são: Manihot esculenta L. (100%, Carica papaya L. (76,2%, Musa paradisiaca L. (71,4%, Saccharum officinarum L. (57,1%. Na mata de galeria destacou-se a categoria medicinal (65%, com as espécies: Copaifera langsdorffii Desf. (2,5, Aspidosperma polyneuron Muell. Arg. (2,5, Hymenaea stignocarpa Mart. (2,33, Diptychandra aurantiaca Tul. (2,0, Cariniana rubra Gardner ex. Miers (20 e as famílias botânicas Mimosaceae, Bignoniaceae, Caesalpiniaceae, Fabaceae e Sapindaceae. Os resultados demonstram que a população possui vasto conhecimento das plantas e de suas propriedades de cura.This paper aims at doing the ethnobotany survey of plants classified in different categories of use in the community of Conceição-Açu, in the city of Cuiabá, MT, Brazil and estimate the value of use of the species botanic in the gallery forest. Structured and non-structured interviews and the questionnaire were used in 59 adult dwellers of both sexes. Were identified the landscape units: backyards, husbandry and gallery forest and the total number of used species is the 180. The majority of the plants was collected during the interview and is kept in the

  5. Composição mineral de uma linhagem de Pleurotus ostreatus cultivada em resíduos madeireiros e agroindustriais da região amazônica Mineral composition of Pleurotus ostreatus strain grown in wood and agroindustrial residues from the Amazon region

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    Ceci Sales-Campos

    2009-12-01

    Full Text Available Os cogumelos do gênero Pleurotus são cultivados em diversos substratos lignocelulósicos, dada a atividade decompositora desses organismos proveniente de seu metabolismo enzimático. O presente estudo teve como objetivo analisar a composição mineral de Pleurotus ostreatus e dos substratos de cultivo preparados à base de resíduos madeireiros e agroindustriais da região amazônica. Foram analisados macro (P, K, Ca e Mg e micronutrientes (Fe, Zn, Cu, Mn e Na dos cogumelos e dos substratos. Os substratos foram formulados a partir da serragem de Simarouba amara Aubl. (marupá, Ochroma piramidale Cav. ex. Lam. (pau de balsa e de bagaços de Bactris gasipaes Kunth (pupunheira e de Saccharum officinarum (cana-de-açúcar. As amostras foram solubilizadas mediante digestão ácida (nítrico-peridrol. Os elementos Ca, Mg, Fe, Cu, Zn e Mn foram determinados por espectrometria de absorção atômica; o Na e K, por emissão atômica e o P, por colorimetria. A composição mineral do cogumelo variou com o substrato de cultivo. Os diferentes substratos possibilitaram a produção de um cogumelo rico em K, P, Mg e Fe, essenciais à nutrição e à saúde humana. O potássio foi o mineral de maior teor no cogumelo em todos os substratos testados (36,83-42,18 g.kg-1, seguido de fósforo (6,95-10,60 g.kg-1 e do magnésio (1,57-2,50 g.kg-1.Mushrooms belonging to the Pleurotus gender are grown in several lignocellulosic substrates due to the decomposing activity of these organisms that result from their enzymatic metabolism. The objective of the present study was to analyze the mineral composition of Pleurotus ostreatus and the cultivation substrates prepared with wood and agroindustrial residues from the Amazon region. Macro (P, K, Ca and Mg and micronutrients (Fe, Zn, Cu, Mn and Na of mushroom and substrates were analyzed. Substrates were formulated from Simarouba amara Aubl. and Ochroma piramidale Cav. ex. Lam. sawdust and crushed Bactris gasipaes Kunth

  6. Subtractive hybridization-mediated analysis of genes and in silico prediction of associated microRNAs under waterlogged conditions in sugarcane (Saccharum spp.

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    Mohammad Suhail Khan

    2014-01-01

    Full Text Available Sugarcane is an important tropical cash crop meeting 75% of world sugar demand and it is fast becoming an energy crop for the production of bio-fuel ethanol. A considerable area under sugarcane is prone to waterlogging which adversely affects both cane productivity and quality. In an effort to elucidate the genes underlying plant responses to waterlogging, a subtractive cDNA library was prepared from leaf tissue. cDNA clones were sequenced and annotated for their putative functions. Major groups of ESTs were related to stress (15%, catalytic activity (13%, cell growth (10% and transport related proteins (6%. A few stress-related genes were identified, including senescence-associated protein, dehydration-responsive family protein, and heat shock cognate 70 kDa protein. A bioinformatics search was carried out to discover novel microRNAs (miRNAs that can be regulated in sugarcane plants subjected to waterlogging stress. Taking advantage of the presence of miRNA precursors in the related sorghum genome, seven candidate mature miRNAs were identified in sugarcane. The application of subtraction technology allowed the identification of differentially expressed sequences and novel miRNAs in sugarcane under waterlogging stress. The comparative global transcript profiling in sugarcane plants undertaken in this study suggests that proteins associated with stress response, signal transduction, metabolic activity and ion transport play important role in conferring waterlogging tolerance in sugarcane.

  7. Efecto de la profundidad del suelo en rottboellia cochinchinensis (lour clayton en caña de azúcar (saccharum officinarum l.

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    Ram\\u00F3n Le\\u00F3n

    2001-01-01

    Full Text Available Se muestrearon cuatro lotes con diferente textura de suelo, sembrados con caña de azúcar durante cuatro años consecutivos. El lote A con suelo franco arcilloso, el lote B con suelo franco arenoso, el lote C con suelo arcilloso y el lote D con suelo franco. Se seleccionaron lotes con una población de R. cochinchinensis de 75 a 150 plantas/ m2. Se contó el número de plantas de la maleza en un área de 0,25 m2, en dos micro-hábitats: el surco y entre-surco. A cada planta se le escarbó el suelo que la rodeaba hasta encontrar la semilla de la que provenía y se midió la profundidad a la que ésta se encontró, para luego determinar qué porcentaje del total de plantas contabilizadas provenía de semillas localizadas en cada uno de los siguientes estratos: 0,0; >0,0 a 2,5; >2,5 a 5,0; >5,0 a 10 y >10,0 a 15,0 cm de profundidad. Además, se evaluó en invernadero el efecto de cada profundidad de los estratos citados. A nivel de laboratorio y en platos Petri, se evaluó el efecto de la luz y la oscuridad sobre la germinación de la semilla de R. cochinchinensis. Se encontró que la mayoría de las plantas en campo provenían de semillas que se encontraban de >0 a 2,5 cm, seguido por aquellas que estaban de >2,5 a 5,0 cm donde se dieron las mejores condiciones para la germinación. No se observaron diferencias entre los tipos de suelo o los micro-hábitats evaluados. En invernadero el tratamiento a 0,0 cm fue el que mostró la mayor germinación y esto fue producto de la exposición a la luz, lo cual se corroboró en el estudio en laboratorio donde el tratamiento con luz presentó también, la mayor germinación

  8. Subtractive hybridization-mediated analysis of genes and in silico prediction of associated microRNAs under waterlogged conditions in sugarcane (Saccharum spp.)

    KAUST Repository

    Khan, Mohammad Suhail

    2014-06-09

    Sugarcane is an important tropical cash crop meeting 75% of world sugar demand and it is fast becoming an energy crop for the production of bio-fuel ethanol. A considerable area under sugarcane is prone to waterlogging which adversely affects both cane productivity and quality. In an effort to elucidate the genes underlying plant responses to waterlogging, a subtractive cDNA library was prepared from leaf tissue. cDNA clones were sequenced and annotated for their putative functions. Major groups of ESTs were related to stress (15%), catalytic activity (13%), cell growth (10%) and transport related proteins (6%). A few stress-related genes were identified, including senescence-associated protein, dehydration-responsive family protein, and heat shock cognate 70. kDa protein. A bioinformatics search was carried out to discover novel microRNAs (miRNAs) that can be regulated in sugarcane plants subjected to waterlogging stress. Taking advantage of the presence of miRNA precursors in the related sorghum genome, seven candidate mature miRNAs were identified in sugarcane. The application of subtraction technology allowed the identification of differentially expressed sequences and novel miRNAs in sugarcane under waterlogging stress. The comparative global transcript profiling in sugarcane plants undertaken in this study suggests that proteins associated with stress response, signal transduction, metabolic activity and ion transport play important role in conferring waterlogging tolerance in sugarcane. © 2014 The Authors.

  9. Diversity of the Bacterial Microbiome in the Roots of Four Saccharum Species: S. spontaneum, S. robustum, S. barberi, and S. officinarum

    Directory of Open Access Journals (Sweden)

    Meng Dong

    2018-02-01

    Full Text Available Endophytic bacteria are nearly ubiquitously present in the internal tissues of plants, and some endophytes can promote plant growth. In this study, we sampled the roots of four ancestral species of sugarcane (two genotypes per species and two sugarcane cultivars, and used 16S rRNA and nifH gene sequencing to characterize the root endophytic bacterial communities and diazotroph diversity. A total of 7,198 operational taxonomic units (OTUs were detected for the endophytic bacteria community. The endophytic bacterial communities exhibited significantly different α- and β-diversities. From the 202 detected families in the sugarcane roots, a core microbiome containing 13 families was identified. The nifH gene was successfully detected in 9 of 30 samples from the four sugarcane species assayed, and 1,734 OTUs were merged for endophytic diazotrophs. In the tested samples, 43 families of endophytic diazotrophs were detected, and six families showed differences across samples. Among the 20 most abundant detected genera, 10 have been reported to be involved in nitrogen fixation in sugarcane. These findings demonstrate the diversity of the microbial communities in different sugarcane germplasms and shed light on the mechanism of biological nitrogen fixation in sugarcane.

  10. Effect of the use of molasses and efficient microorganisms, over the rate of decomposition of the sugar cane leaf (Saccharum officinarum

    Directory of Open Access Journals (Sweden)

    Óscar Eduardo Sanclemente Reyes

    2011-10-01

    Full Text Available The rate of decomposition of sugar cane leaves mixed with an organic fertilizer compost type was evaluated, using a finite accelerator (molasses and an infinity accelerator (effective microorganisms. The trial was conducted in the greenhouse facilities of the National University of Colombia in Palmira. The results showed that molasses is a decomposition accelerator of the wastes of sugar cane leaf, since it shows a marked influence on the initial decomposition rate of the waste, but once the carbohydrates that constitute it are consumed, the rate of decomposition decreases significantly. Then the potential is evident on the waste of sugar cane leaf elements for the maintenance and/or biophysical capital improvement in the productive system of the sugar cane, as the result of their high photosynthetic efficiency.

  11. Subtractive hybridization-mediated analysis of genes and in silico prediction of associated microRNAs under waterlogged conditions in sugarcane (Saccharum spp.)

    KAUST Repository

    Khan, Mohammad Suhail; Khraiwesh, Basel; Pugalenthi, Ganesan; Gupta, Ram Sagar; Singh, Jyotsnendra; Duttamajumder, Sanjoy Kumar; Kapur, Raman

    2014-01-01

    Sugarcane is an important tropical cash crop meeting 75% of world sugar demand and it is fast becoming an energy crop for the production of bio-fuel ethanol. A considerable area under sugarcane is prone to waterlogging which adversely affects both

  12. Herbaceous energy crops in humid lower South USA

    Energy Technology Data Exchange (ETDEWEB)

    Prine, G.M.; Woodard, K.R. [Univ. of Florida, Gainesville, FL (United States)

    1993-12-31

    The humid lower South has the long warm growing season and high rainfall conditions needed for producing high-yielding perennial herbaceous grasses and shrubs. Many potential biomass plants were evaluated during a ten-year period. Perennial tall grasses such as elephantgrass (Pennisetum purpureum), sugarcane and energycane (Saccharum spp.) and the leguminous shrub Leucaena leucocephala were the highest in biomass production. These perennial crops often have top growth killed by winter freezes and regenerate from underground parts. The tall grasses have high yields because of linear crop growth rates of 18 to 27 g m{sup 2} d{sup {minus}1} for long periods (140 to 196 d) each season. Tall grasses must be planted vegetatively, which is more costly than seed propagation, however, once established, they may persist for many seasons. Oven dry biomass yields have varied from 20 to 45 Mg ha{sup {minus}1} yr{sup {minus}1} in colder subtropical to mild temperate locations to over 60 Mg ha{sup {minus}1} yr{sup {minus}1} in the lower portion of the Florida peninsular. Highest biomass yields have been produced when irrigated with sewage effluent or when grown on phosphatic clay and muck soils in south Florida. The energy content of 1 Mg of oven dry tall grass and leucaena is equivalent to that of about 112 and 123 gallons of number 2 diesel fuel, respectively.

  13. Application of ecohydraulic bank protection model to improve river bank stability and biotic community in Surabaya River

    Directory of Open Access Journals (Sweden)

    Daru Setyo Rini

    2017-10-01

    Full Text Available Ecohydraulic river bank protection design was developed as ECO-RIPRAP model and has been applied along 100 meter length to restore accelerated erosion sites in Surabaya River at Wringinanom and Klubuk. The model combined re-profiled and re-vegetated bank with rock toe reinforcement and addition of log groynes at 10 meter length interval. Various native plant species were planted on bank slopes, including water plants Ipomoea aquatica and Pistia stratiotes, grasses and shrubs Ipomoea carnea, Pluchea indica, Saccharum spontaneum, Arundo donax, and native tree species Ficus glomerata, Bambusa arundinacea, Dendrocalamus asper, Bambusa vulgaris, Ficus benjamina, Dillenia indica, Psidium guajava, Arthocarpus camansi, Arthocarpus elasticus, Hibiscus mutabilis, Nauclea sp., Inocarpus edulis, and Syzygium polyanthum. The river bank morphology after ECO-RIPRAP application showed alteration from erosion to sedimentation due to rock toe enforcement, log groynes protection, and increase of plant cover on littoral banks that decreased near bank velocity. The macro-invertebrate community shown increase of taxa richness, EPT richness, %EPT and %Atyidae, but decrease of %Chironomidae at restored sites. The fish community shown increase of taxa richness, increase of abundance by 54.2%, increase of Pangasius micronemus abundance by 25.6%, and increase of Hemibragus nemurus abundance by 6.3 % at restored reach. Rare fish species thrive back at restored area, namely Oxyeleotris marmorata, Mastacembelus unicolor and Hampala macrolepidota.

  14. Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation[OPEN

    Science.gov (United States)

    Lowe, Keith; Wu, Emily; Cho, Myeong-Je; Lenderts, Brian; Chamberlin, Mark; Cushatt, Josh; Ryan, Larisa; Khan, Tanveer; Chow-Yiu, Julia; Hua, Wei; Banh, Jenny; Bao, Zhongmeng; Brink, Kent; Igo, Elizabeth; Rudrappa, Bhojaraja; Shamseer, PM; Shen, Bo; Zheng, Peizhong; Bidney, Dennis; Falco, Carl; Zhao, Zuo-Yu; Xu, Deping

    2016-01-01

    While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus. PMID:27600536

  15. Integrated dynamic model of the alkaline delignification process of Lignocellulosic biomass

    International Nuclear Information System (INIS)

    Fuertez, John; Ruiz, Angela; Alvarez, Hernan; Molina, Alejandro

    2011-01-01

    Although in the public literature there are several studies that describe models of alkaline delignification, they were originally developed for the paper industry, and do not include the effects of important operating variables such as temperature, hydroxide-ion concentration, solid to liquid weight ratio, particle size, biomass composition (hemi cellulose, lignin fraction) and mixing. This lack of detailed models of the pretreatment stages prompted the current study that describes a model which includes the variables listed above and provides an important tool for predicting the degree of lignin removal in lignocellulosic materials such as sugar cane bagasse (Saccharum officinarum L). The model considers kinetic expressions available in the literature. The kinetic parameters were determined by fitting the model to experimental data obtained for that purpose in our lab. The experimental matrix considered eighteen, 24-h isothermal experiments in which bulk and residual delignification stages were observed to occur in a parallel manner. Carbohydrate removal and hydroxide consumption were related to lignin removal by effective stoichiometric coefficients that were calculated by fitting the experimental data. A mixing compartment network model that represented mixing inside the reactor was included into a temporal superstructure based on the similarity between plug flow reactors and ideal batch reactors to model a non-ideally mixed batch reactor. The kinetic model was validated with data obtained in this study.

  16. GRAMÍNEAS TROPICALES EN EL ENGORDE DE CUYES MEJORADOS SEXADOS (Cavia porcellus Linnaeus EN LA ZONA DE LA MANÁ

    Directory of Open Access Journals (Sweden)

    Adolfo Sánchez Laiño

    2009-06-01

    Full Text Available La investigación de gramíneas tropicales en el engorde de cuyes en la zona de La Maná. La misma que persiguió los siguientes objetivos: Incrementar los índices productivos en cuyes (Cavia porcellus Linnaeus peruanos mejorados bajo el efecto del consumo del pasto saboya (Panicum maximun Jack, hojas de maíz (Zea mays y caña de azúcar (Saccharum officinarum L en la zona de La Maná, y determinar la rentabilidad de los tratamientos. Se utilizaron 48 cuyes peruanos mejorados sexados de 35 días de edad con un peso promedio de 305 g. Se aplicó un arreglo factorial 2 (sexo x 3 (gramíneas con cuatro repeticiones, dentro de un (DBCA. Para las comparaciones entre medidas se utilizó la prueba de Tukey al 0.05% de probabilidad. Para obtener la rentabilidad de los tratamientos se utilizó la Relación Beneficio–Costo. Se evaluó el: consumo de gramíneas (g, consumo de alimento (g, ganancia de peso (g, conversión alimenticia, peso vivo (g y rendimiento a la canal (%. Los cuyes machos presentaron el mayor (P0.05 no se vio afectado por el efecto del consumo de gramíneas. La mayor rentabilidad la registraron los cuyes machos alimentados con hoja de maíz + balanceado (52.4%.

  17. A bayesian approach to inferring the genetic population structure of sugarcane accessions from INTA (Argentina

    Directory of Open Access Journals (Sweden)

    Mariana Inés Pocovi

    2015-06-01

    Full Text Available Understanding the population structure and genetic diversity in sugarcane (Saccharum officinarum L. accessions from INTA germplasm bank (Argentina will be of great importance for germplasm collection and breeding improvement as it will identify diverse parental combinations to create segregating progenies with maximum genetic variability for further selection. A Bayesian approach, ordination methods (PCoA, Principal Coordinate Analysis and clustering analysis (UPGMA, Unweighted Pair Group Method with Arithmetic Mean were applied to this purpose. Sixty three INTA sugarcane hybrids were genotyped for 107 Simple Sequence Repeat (SSR and 136 Amplified Fragment Length Polymorphism (AFLP loci. Given the low probability values found with AFLP for individual assignment (4.7%, microsatellites seemed to perform better (54% for STRUCTURE analysis that revealed the germplasm to exist in five optimum groups with partly corresponding to their origin. However clusters shown high degree of admixture, F ST values confirmed the existence of differences among groups. Dissimilarity coefficients ranged from 0.079 to 0.651. PCoA separated sugarcane in groups that did not agree with those identified by STRUCTURE. The clustering including all genotypes neither showed resemblance to populations find by STRUCTURE, but clustering performed considering only individuals displaying a proportional membership > 0.6 in their primary population obtained with STRUCTURE showed close similarities. The Bayesian method indubitably brought more information on cultivar origins than classical PCoA and hierarchical clustering method.

  18. Use of Temporary Immersion System (RITA in sugarcane the plants propagation by organogenesis

    Directory of Open Access Journals (Sweden)

    Laisyn Posada-Pérez

    2003-01-01

    Full Text Available The present work had as objective to study the possibilities to use the RITA, in the micropropagation plant via axillary buds of sugarcane and of the banana hybrid (Musa sp. AAAA cv. FHIA-01 and AAAB cv. FHIA-18. Two different culture medium i.e. multiplication and thickenment were studied in the multiplication phase of two varieties of sugarcane. It was also studied the number of initial explants to use (two, four and six, using as control flasks with explants liquid and semisolid medium. The number of immersions in 24 hours was analysed and also the time for culture media change was determined testing two periods, 15 days and 30 days respectively. Similar variables were studied for the case of the banana cultivar except for the medium culture. It was obtained like half of more appropriate cultivation for sugarcane that of multiplication with two explants, in the two used varieties, not presenting significant differences the times of change of half made to the 15 and 30 days. For the case of the banana cultivar employment of five explantes for FHIA-01 and three or five explantes for FHIA-18 to begin the multiplication allowed to reach the best results. Regarding the number of immersions it presented bigger superiority the treatment of three immersions during 24 hours, for both banana cultivars. Keys words: liquid medium, micropropagation, Musa, Saccharum

  19. Classification, expression pattern and comparative analysis of sugarcane expressed sequences tags (ESTs encoding glycine-rich proteins (GRPs

    Directory of Open Access Journals (Sweden)

    Fusaro Adriana

    2001-01-01

    Full Text Available Since the isolation of the first glycine-rich proteins (GRPs in plants a wealth of new GRPs have been identified. The highly specific but diverse expression pattern of grp genes, taken together with the distinct sub-cellular localization of some GRP groups, clearly indicate that these proteins are involved in several independent physiological processes. Notwithstanding the absence of a clear definition of the role of GRPs in plant cells, studies conducted with these proteins have provided new and interesting insights into the molecular biology and cell biology of plants. Complexly regulated promoters and distinct mechanisms for the regulation of gene expression have been demonstrated and new protein targeting pathways, as well as the exportation of GRPs from different cell types have been discovered. These data show that GRPs can be useful as markers and/or models to understand distinct aspects of plant biology. In this paper, the structural and functional features of these proteins in sugarcane (Saccharum officinarum L. are summarized. Since this is the first description of GRPs in sugarcane, special emphasis has been given to the expression pattern of these GRP genes by studying their abundance and prevalence in the different cDNA-libraries of the Sugarcane Expressed Sequence Tag (SUCEST project . The comparison of sugarcane GRPs with GRPs from other species is also discussed.

  20. MOSSES AS BIOINDICATORS OF AIR POLLUTION ALONG AN URBAN–AGRICULTURAL TRANSECT IN THE CREDIT RIVER WATERSHED, SOUTHERN ONTARIO, CANADA

    Directory of Open Access Journals (Sweden)

    P. Cowden

    2015-04-01

    Full Text Available The activities associated with urbanization, such as vehicular traffic and industrial processes, lead to elevated emissions of atmospheric pollutants. Measuring the spatial extent of these pollutants is pivotal to identifying areas of concern and assessing mitigation measures. The objective of this study was to evaluate the relative deposition of heavy metals and nitrogen using moss species along an urban–agricultural transition in the Credit River Watershed, southern Ontario. Thirteen species of moss were collected from Sugar Maple (Acer saccharum dominated forest stands across the study area, with only one moss species (Atrichum altercristatum commonly occurring. Heavy metal concentrations were variable between species; the Coefficient of Variation (CV for the majority of metals (Al, V, Cr, Fe, Ni, As, Sb and Pb was greater than ~50% across species. Nonetheless, metals exhibited similar trends, with the highest concentrations for Fe, followed by Al > Zn > Cu > Pb > Cr > Ni > V > As > Cd > Sb > Hg across species. Heavy metal concentrations in Atrichum altercristatum exhibited lower variability between sites, with CV < 33% for most metals (Cu, Zn, As, Cd, Sb, Pb and Hg. Further, many metal concentrations were strongly correlated (e.g., Al, V, Cr, Fe, and As; r ≤ 0.90 suggesting common emission sources, such as wind blown dust from agricultural activities or vehicular traffic, both predominant throughout the watershed.

  1. PlantRNA_Sniffer: A SVM-Based Workflow to Predict Long Intergenic Non-Coding RNAs in Plants.

    Science.gov (United States)

    Vieira, Lucas Maciel; Grativol, Clicia; Thiebaut, Flavia; Carvalho, Thais G; Hardoim, Pablo R; Hemerly, Adriana; Lifschitz, Sergio; Ferreira, Paulo Cavalcanti Gomes; Walter, Maria Emilia M T

    2017-03-04

    Non-coding RNAs (ncRNAs) constitute an important set of transcripts produced in the cells of organisms. Among them, there is a large amount of a particular class of long ncRNAs that are difficult to predict, the so-called long intergenic ncRNAs (lincRNAs), which might play essential roles in gene regulation and other cellular processes. Despite the importance of these lincRNAs, there is still a lack of biological knowledge and, currently, the few computational methods considered are so specific that they cannot be successfully applied to other species different from those that they have been originally designed to. Prediction of lncRNAs have been performed with machine learning techniques. Particularly, for lincRNA prediction, supervised learning methods have been explored in recent literature. As far as we know, there are no methods nor workflows specially designed to predict lincRNAs in plants. In this context, this work proposes a workflow to predict lincRNAs on plants, considering a workflow that includes known bioinformatics tools together with machine learning techniques, here a support vector machine (SVM). We discuss two case studies that allowed to identify novel lincRNAs, in sugarcane ( Saccharum spp.) and in maize ( Zea mays ). From the results, we also could identify differentially-expressed lincRNAs in sugarcane and maize plants submitted to pathogenic and beneficial microorganisms.

  2. Biomass chemicals: improvement in quality and quantity with physiological regulators

    Energy Technology Data Exchange (ETDEWEB)

    Kossuth, S.V.

    1984-01-01

    The search for alternative biomass energy forms has centered on two approaches: (1) production of cellulose fiber in biomass of low net energy value per unit weight, such as wood and bagasse, and (2) hydrocarbons of high net energy value per unit weight for use as chemical feedstocks and substitutes for petroleum. Major plant chemical products include oleoresin from pine (Pinus elliottii Engelm., P. palustris Mill.) rubber from the rubber tree (Hevea brasiliensis Muell.), and guayule shrub (Parthenium argentatum Gray) and sugar from sugarcane (Saccharum species). Ethylene may be a unifying natural bioregulator that can increase deposition of biomass chemicals in all four of these systems. Examples of bioregulators include the use of paraquat, diquat, and 2-chloroethylphosphonic acid (CEPA) for stimulating the synthesis of oleoresin, CEPA for prolonging the flow of rubber and increasing rubber synthesis in the rubber tree, and triethylamines of chlorinated phenoxy compounds for stimulating rubber production in guayule. In sugarcane, gibberellic acid (GA3) increases internodal elongation. Glyphosate, CEPA and other regulators increase the deposition of sucrose, diquat and CEPA inhibit flowering, and paraquat desiccates leaves to facilitate leaf removal or burning just prior to harvest. The cellular compartmentalization for the synthesis of these plant chemicals is unique for each species, and dictates cultural and harvest techniques. The mode of action and pathways for the success of these physiological regulators are discussed. 42 references.

  3. Effects of maple (Acer) plant part extracts on proliferation, apoptosis and cell cycle arrest of human tumorigenic and non-tumorigenic colon cells.

    Science.gov (United States)

    González-Sarrías, Antonio; Li, Liya; Seeram, Navindra P

    2012-07-01

    Phenolic-enriched extracts of maple sap and syrup, obtained from the sugar and red maple species (Acer saccharum Marsh, A. rubrum L., respectively), are reported to show anticancer effects. Despite traditional medicinal uses of various other parts of these plants by Native Americans, they have not been investigated for anticancer activity. Here leaves, stems/twigs, barks and sapwoods of both maple species were evaluated for antiproliferative effects against human colon tumorigenic (HCT-116, HT-29, Caco-2) and non-tumorigenic (CCD-18Co) cells. Extracts were standardized to total phenolic and ginnalin-A (isolated in our laboratory) levels. Overall, the extracts inhibited the growth of the colon cancer more than normal cells (over two-fold), their activities increased with their ginnalin-A levels, with red > sugar maple extracts. The red maple leaf extract, which contained the highest ginnalin-A content, was the most active extract (IC₅₀  = 35 and 16 µg/mL for extract and ginnalin-A, respectively). The extracts were not cytotoxic nor did they induce apoptosis of the colon cancer cells. However, cell cycle analyses revealed that the antiproliferative effects of the extracts were mediated through cell cycle arrest in the S-phase. The results from the current study suggest that these maple plant part extracts may have potential anticolon cancer effects. Copyright © 2011 John Wiley & Sons, Ltd.

  4. How fresh is maple syrup? Sugar maple trees mobilize carbon stored several years previously during early springtime sap-ascent.

    Science.gov (United States)

    Muhr, Jan; Messier, Christian; Delagrange, Sylvain; Trumbore, Susan; Xu, Xiaomei; Hartmann, Henrik

    2016-03-01

    While trees store substantial amounts of nonstructural carbon (NSC) for later use, storage regulation and mobilization of stored NSC in long-lived organisms like trees are still not well understood. At two different sites with sugar maple (Acer saccharum), we investigated ascending sap (sugar concentration, δ(13) C, Δ(14) C) as the mobilized component of stored stem NSC during early springtime. Using the bomb-spike radiocarbon approach we were able to estimate the average time elapsed since the mobilized carbon (C) was originally fixed from the atmosphere and to infer the turnover time of stem storage. Sites differed in concentration dynamics and overall δ(13) C, indicating different growing conditions. The absence of temporal trends for δ(13) C and Δ(14) C indicated sugar mobilization from a well-mixed pool with average Δ(14) C consistent with a mean turnover time (TT) of three to five years for this pool, with only minor differences between the sites. Sugar maple trees hence appear well buffered against single or even several years of negative plant C balance from environmental stress such as drought or repeated defoliation by insects. Manipulative investigations (e.g. starvation via girdling) combined with Δ(14) C measurements of this mobilized storage pool will provide further new insights into tree storage regulation and functioning. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  5. Potential of fluorescence spectroscopy for the characterisation of maple syrup flavours.

    Science.gov (United States)

    Panneton, Bernard; Clément, Alain; Lagacé, Luc

    2013-10-01

    Maple syrup has high maket value. It is produced in North East America from the heat-evaporated sap of Acer saccharum Marshall. For marketing purposes, there is interest in defining its flavour profile in a consistent and repeatable manner. An experiment was undertaken to explore the potential of autofluorescence of maple syrup induced at 275 and 360 nm to characterise flavours. A mixed data factor analysis revealed two independent groups of variables. One represents early season woody and late season empyreumatic flavours. The other is related to off-flavour, confectionery and maple flavours. Maple and confectionery flavours are subtle, difficult to distinguish and opposed to off-flavour. There were clear relationships among the two groups and fluorescence profiles. For each of the five basic flavours, discriminant models based on partial least squares regressions were developed. For each sample of syrup, flavours combined to form flavour profiles, and the results from the five discriminant models were aggregated to reproduce these profiles. For excitation at 275 nm, the woody/off-flavour and confectionery/empyreumatic/maple flavour profiles were classified correctly 86 and 78% of the time (cross-validation) respectively. Induced autofluorescence spectra were shown to contain information related to maple syrup flavours. This fluorescence-flavour relationship is not considered quantitative yet, and further research avenues are proposed. © 2013 Society of Chemical Industry.

  6. PlantRNA_Sniffer: A SVM-Based Workflow to Predict Long Intergenic Non-Coding RNAs in Plants

    Directory of Open Access Journals (Sweden)

    Lucas Maciel Vieira

    2017-03-01

    Full Text Available Non-coding RNAs (ncRNAs constitute an important set of transcripts produced in the cells of organisms. Among them, there is a large amount of a particular class of long ncRNAs that are difficult to predict, the so-called long intergenic ncRNAs (lincRNAs, which might play essential roles in gene regulation and other cellular processes. Despite the importance of these lincRNAs, there is still a lack of biological knowledge and, currently, the few computational methods considered are so specific that they cannot be successfully applied to other species different from those that they have been originally designed to. Prediction of lncRNAs have been performed with machine learning techniques. Particularly, for lincRNA prediction, supervised learning methods have been explored in recent literature. As far as we know, there are no methods nor workflows specially designed to predict lincRNAs in plants. In this context, this work proposes a workflow to predict lincRNAs on plants, considering a workflow that includes known bioinformatics tools together with machine learning techniques, here a support vector machine (SVM. We discuss two case studies that allowed to identify novel lincRNAs, in sugarcane (Saccharum spp. and in maize (Zea mays. From the results, we also could identify differentially-expressed lincRNAs in sugarcane and maize plants submitted to pathogenic and beneficial microorganisms.

  7. Agroindustrial Waste for Lead and Chromium Biosorption

    Directory of Open Access Journals (Sweden)

    Susana P. Boeykens

    2018-06-01

    Full Text Available There is a need to re-evaluate the residues generated in industrial processes for the production of new raw material, reducing the volume of waste. In this regard, the biosorption is a low-cost alternative method for treating effluents compared to conventional methods. The main objectives of this research were: the evaluation of the biosorbent capacity of six waste materials for the extraction of chromium(VI and lead(II ions from aqueous solutions and, the determination of the adsorption and kinetic parameters for the more efficient system. The materials evaluated were: peanut shell (Arachis hypagaea, sugarcane bagasse (Saccharum officinarum, avocado peel (Persea americana, pecan nutshell (Carya illinoinensis, wheat bran (Triticum aestivum and banana peel (Mussa paradisiaca. The highest percentage of lead removal was obtained with wheat bran (89%. For chromium, the percentage was generally much lower compared with lead for all tested biosorbents, the banana peel being the most efficient with a 10% removal. The models that better describe the adsorption processes were: Langmuir and Freundlich. The pseudo-second order kinetic model allowed obtaining the parameters for both systems. The equilibrium time, in both systems, was reached after 60 minutes. The study of Fourier Transformed Infrared spectra and the results of desorption experiments allowed to hypothesize on the mechanisms involved in the adsorption of these metals.

  8. Estrategias metodológicas para el análisis de la reconversión y diversificación productiva de regiones cañeras

    Directory of Open Access Journals (Sweden)

    Noé Aguilar Rivera

    2017-01-01

    Full Text Available El azúcar ha sido el principal producto comercial de la caña de azúcar (Saccharum officinarum. La producción del edulcorante en México depende de tres factores: a cantidad y calidad de caña de azúcar industrializada; b rendimiento de la fábrica; y c capacidad instalada y de molienda de ingenios azucareros. Sin embargo, el análisis de factores de producción que afectan su competitividad (productividad y capacidad de diversificación se ha llevado a cabo sin considerar la distribución espacial y temporal, aptitud agroecológica de las regiones cañeras y factores limitantes socioeconómicos. Este trabajo identificó mediante el método multicriterio AHP (Jerarquías analíticas en sistemas de información geográfica y técnicas participativas, como herramientas para el análisis de sistemas complejos, donde los factores clave Aptitud agroclimática al cultivo de caña de azúcar; Rendimiento de campo; y Tamaño de la unidad de producción explican el 78 % del potencial de regiones cañeras para establecer proyectos de diversificación y/o reconversión productiva. Sin embargo, para los productores cañeros la diversificación es un tema conocido, pero poco atractivo.

  9. Concentration of radionuclides in uranium tailings and its uptake by plants at Jaduguda, Jharkhand, India

    International Nuclear Information System (INIS)

    Singh, Lal; Soni, Prafulla

    2010-01-01

    Mining and processing of uranium ore was started in several parts of eastern Singhbhum, viz. Jaduguda, Bhatin and Narwapahar (Jharkhand) in 1968. Radioactivity in the mine tailings has to be consolidated so that it does not emanate in the atmosphere or enter the food chain. Hence, the area has been covered with 30 cm thick soil cover followed by development of plant species that do not have any socioeconomic relevance in the area. Seven native plant species of forestry origin, viz. Colebrookea oppositifolia, Dodonaea viscosa, Furcraea foetida, Imperata cylindrica, Jatropha gossypifolia, Pogostemon benghalense and Saccharum spontaneum have been selected for experimental trials. Distribution and concentration of radionuclides have been evaluated in a tailing pond at different depths in soil and tailings. Radionuclide uptake in each of the selected plant species has been evaluated and discussed in this article. The highest concentration of radionuclides has been found in tailings > soil cover on tailings > roots of selected plant species > shoots of all the selected species. These results show that among the seven species tried, J. gossypifolia and F. foetida have the lowest uptake (below detectable limits), while S. spontaneum and P. benghalense have comparatively higher uptake. However, radionuclide concentration in all the tried species is significantly low compared to species of natural occurrence which have higher radionuclides uptake and accumulation. (author)

  10. Concentration of radionuclides in uranium tailings and its uptake by plants at Jaduguda, Jharkhand, India

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Lal; Soni, Prafulla [Ecology and Environment Div., Forest Research Institute, Dehradun (India)

    2010-01-10

    Mining and processing of uranium ore was started in several parts of eastern Singhbhum, viz. Jaduguda, Bhatin and Narwapahar (Jharkhand) in 1968. Radioactivity in the mine tailings has to be consolidated so that it does not emanate in the atmosphere or enter the food chain. Hence, the area has been covered with 30 cm thick soil cover followed by development of plant species that do not have any socioeconomic relevance in the area. Seven native plant species of forestry origin, viz. Colebrookea oppositifolia, Dodonaea viscosa, Furcraea foetida, Imperata cylindrica, Jatropha gossypifolia, Pogostemon benghalense and Saccharum spontaneum have been selected for experimental trials. Distribution and concentration of radionuclides have been evaluated in a tailing pond at different depths in soil and tailings. Radionuclide uptake in each of the selected plant species has been evaluated and discussed in this article. The highest concentration of radionuclides has been found in tailings > soil cover on tailings > roots of selected plant species > shoots of all the selected species. These results show that among the seven species tried, J. gossypifolia and F. foetida have the lowest uptake (below detectable limits), while S. spontaneum and P. benghalense have comparatively higher uptake. However, radionuclide concentration in all the tried species is significantly low compared to species of natural occurrence which have higher radionuclides uptake and accumulation. (author)

  11. Bioremediation studies of tailing ponds of uranium mines

    International Nuclear Information System (INIS)

    Sudhakar, G.; Muralidhar Rao, C.; Swaminathan, Siva Kumar

    2012-01-01

    A study was undertaken for three years to evaluate the potential of native plant species for the phytoremediation of tailing ponds of Uranium mines, Jaduguda, Jharkhand. Five sampling stations: three at Jaduguda (TP1, TP2, and TP3), one at Turamdih (TTP) and one at Effluent Treatment Plant (ETP) were selected. pH, Electrical conductivity (EC), 12 metals (- AI, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Cd, Pb) and 3 radionuclides - Co, Sr and U) were analysed. From the analysis of sediment/soil/water/effluent of tailing ponds, 4 elements - U, Mn, AI and Fe were found to be significantly in higher concentrations in water, and 8-elements (U, Mn, V, Fe, Ni, Cu and Zn) were found to be in higher concentrations in soils. U and Mn were found to be the predominant contaminants. 26 plant species were screened for their ability to accumulate and remediate the contaminated soils of which only four plant species - one fern (Pteris vittata - P. vittata), one terrestrial (Saccharum spontaneum - S. spontaneum ) and two aquatic species (Typha latifolia - T. latifolia, Cyperus compressus - C. compressus) were shortlisted for phytoremediation studies in laboratory condition and transfer factors were calculated. The results of the study under controlled conditions indicate that P. vittata, S. spontaneum, T. latifolia and C. compressus were found to be the candidate species for phytoremediation of Uranium mine tailings. (author)

  12. Pré-condicionamento in vitro de plantas de cana-de-açúcar (Saccharum spp. para tolerância ao estresse salino

    Directory of Open Access Journals (Sweden)

    Gemima M. de Melo

    Full Text Available RESUMO Avaliou-se a capacidade de adaptação à salinidade de mudas de cana-de-açúcar da variedade RB98710 após pré-condicionamento (priming in vitro com cloreto de sódio (NaCl. O experimento foi conduzido no período de maio a outubro de 2013. Plantas foram micropropagadas em biorreatores de imersão temporária, com tratamentos de priming caracterizados pela adição de 25 mM de NaCl, durante 0, 12, 24 e 36 horas. Concluídos os períodos de priming, as plantas foram cultivadas em meio de enraizamento sem NaCl, aclimatizadas e submetidas a tratamento salino gradativo: S0 = sem NaCl; S1 = regas com 20→40→60 mM de NaCl acrescido à solução nutritiva, durante 30 dias. Procedeu-se à supressão do NaCl e avaliaram-se as plantas 15 dias depois. As variáveis foram: matéria seca da parte aérea (MSPA e radicular (MSR, número de perfilhos, estimativa de clorofila (Chl, conteúdo hídrico relativo e dano de membrana. As plantas pré-condicionadas não reduziram a MSPA quando submetidas ao NaCl. O efeito do estresse salino sobre a MSR foi anulado nas plantas submetidas ao priming por 24 h. O priming favoreceu o perfilhamento das plantas dos tratamentos por 24 ou 36 h. A recuperação após o estresse, avaliado 15 dias após a supressão do NaCl, não diferiu entre plantas submetidas ou não ao priming. De acordo com os resultados, o priming utilizado na variedade RB98710, pode favorecer a tolerância à salinidade.

  13. Direct saponification preparation and analysis of free and conjugated phytosterols in sugarcane (Saccharum officinarum L.) by reversed-phase high-performance liquid chromatography.

    Science.gov (United States)

    Feng, Simin; Liu, Songbai; Luo, Zisheng; Tang, Kaichen

    2015-08-15

    A simple method based on direct saponification followed by RP-HPLC analysis was developed for quantification of free and conjugated sterols in sugarcane. Acid hydrolysis prior to alkaline saponification was used to determined acylated steryl glycoside and steryl glycoside in sugarcane. The applicability and generality of this method were improved with intensive investigation. Compared to traditional solvent extraction method, this method was more time saving and appropriate for characterization of sterol fractions in sugarcane. This method was successfully applied for determination of free and conjugated sterols in different sugarcane samples. The results exhibited that stigmasterol (varied from 883.3 ± 23.5 to 1823.9 ± 24.5 μg/g dry weigh) and β-sitosterol (varied from 117.6 ± 19.9 to 801.4 ± 33.5 μg/g dry weight) were major phytosterols in the sugarcane sample, and their glycosylated forms accounted for almost 87.0% of stigmasterol and 87.5% of β-sitosterol in sugarcane, respectively. In addition, among other parts of sugarcane, tips contained the greatest amount of phytosterols. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Sucrose and color profiles in sugarcane (Saccharum sp.) juice analyzed by UFLC-ELSD and Synapt High-Definition Mass Spectrometry during radiation treatment

    Science.gov (United States)

    Lima, Roberta B.; de Aguiar, Claudio Lima; Galaverna, Renan; Baptista, Antonio S.; Eberlin, Marcos N.; Arthur, Valter

    2016-04-01

    This work evaluated the effect of electron beam irradiation (E-beam) on sugarcane juice and compared the results with preliminary tests performed on sugarcane juice treated with gamma irradiation. The samples were irradiated at 5, 10 and 20 kGy doses and results were compared wile control samples without irradiation. The results showed a significant increase (p≤0.05) of phenolic compounds in both treatments. We also observed increased contents of reducing sugars (glucose and fructose) for the samples irradiated with gamma rays and E-beam measured by the DNS methods. However, there was no significant difference of sugars content measured by chromatographic analyses performed in the sugarcane juice treated with E-beam. Therefore reducing sugars content could be overestimated by the DNS method because salts in sugarcane juice. The treatments were able to reduce sugarcane juice ICUMSA color intensity in both treatments with irradiation. E-beam reduced sugarcane juice color by roughly 49% compared the control, while gamma irradiation reduced it by 30%.

  15. Sucrose and color profiles in sugarcane (Saccharum sp.) juice analyzed by UFLC-ELSD and Synapt High-Definition Mass Spectrometry during radiation treatment

    International Nuclear Information System (INIS)

    Lima, Roberta B.; Lima de Aguiar, Claudio; Galaverna, Renan; Baptista, Antonio S.; Eberlin, Marcos N.; Arthur, Valter

    2016-01-01

    This work evaluated the effect of electron beam irradiation (E-beam) on sugarcane juice and compared the results with preliminary tests performed on sugarcane juice treated with gamma irradiation. The samples were irradiated at 5, 10 and 20 kGy doses and results were compared wile control samples without irradiation. The results showed a significant increase (p≤0.05) of phenolic compounds in both treatments. We also observed increased contents of reducing sugars (glucose and fructose) for the samples irradiated with gamma rays and E-beam measured by the DNS methods. However, there was no significant difference of sugars content measured by chromatographic analyses performed in the sugarcane juice treated with E-beam. Therefore reducing sugars content could be overestimated by the DNS method because salts in sugarcane juice. The treatments were able to reduce sugarcane juice ICUMSA color intensity in both treatments with irradiation. E-beam reduced sugarcane juice color by roughly 49% compared the control, while gamma irradiation reduced it by 30%. - Highlights: • E-beam and γ-radiation were evaluated on color reduction of sugarcane juice. • Any severe degradation of sucrose content was perceived by E-beam or γ-radiation. • Little degradation of sucrose was associated to the increase on radiation doses. • Average color reduction was found to be 49% with E-beam and 30% with γ-radiation. • Synapt HDMS was able to confirm the presence of sucrose, glucose and fructose.

  16. A search for markers of sugarcane evolution

    Directory of Open Access Journals (Sweden)

    M. Bacci Jr.

    2001-12-01

    Full Text Available To determine the phylogenetic relationship between sugarcane cultivars and other members of the Saccharinae subtribe, we identified the fast evolving ITS1-5.8S-ITS2 (ITS = internal transcribed spacer; 5.8S = 5.8S ribosomal DNA region of the sugarcane genome in the Sugarcane Expressed Sequence Tag (SUCEST genome project database. Parsimony analysis utilizing this region and homologs belonging to the 23 closely related Andropogoneae currently deposited in the GenBank database has shown sugarcane as the sister group of Saccharum sinense. However, because there are few parsimony-informative characters and high homoplasy in the ITS1-5.8S-ITS2 region we were not able to determine with confidence the phylogenetic relationship between sugarcane and some of the remaining members of Saccharine subtribe. To find alternatives for the phylogenetic reconstruction of sugarcane evolutionary history, we selected 17 markers (nuclear, chloroplastic or mitochondrial from the SUCEST database of which apha-tubulin, ribosomal protein L16 (rpl16 and DNA-directed RNA polymerase beta chain (rpoC2 were found to have a low incidence of polymorphism and comparable, or even faster, rates of evolution than the ITS1-5.8S-ITS2 region. We suggest that these markers should be considered as preferential choices for phylogenetic studies of Saccharinae subtribe.Com o propósito de determinar a relação filogenética entre a cana-de-açúcar e membros da subtribo Saccharinae, a região gênica nuclear ITS1-5,8S-ITS2 (ITS: espaçador interno transcrito; 5,8S: DNA ribossomal 5.8S, com alta taxa evolutiva, foi identificada no banco de dados do projeto genoma "Sugarcane Expressed Sequence Tag" (SUCEST. Uma análise através do método de parcimônia, utilizando esta região e seqüências homólogas de 23 Andropogoneae retiradas da base de dados GenBank, indicou que a cana-de-açúcar é o grupo-irmão de Saccharum sinense. No entanto, devido à pequena quantidade de caracteres

  17. Fermentation, losses, and aerobic stability of sugarcane silages treated with chemical or bacterial additives Fermentação, perdas e estabilidade aeróbia de silagens de cana-de-açúcar tratadas com aditivos químicos ou bacterianos

    Directory of Open Access Journals (Sweden)

    André de Faria Pedroso

    2008-12-01

    Full Text Available Utilization of sugarcane Saccharum officinarum L. silage is increasing in Brazil but intensive ethanol production during fermentation reduces forage quality. This experiment aimed to evaluate the effects of additives on fermentation and aerobic stability of sugarcane silages produced in minisilos. Treatments were (fresh basis: untreated silage (control, urea (5.0 g kg-1, sodium benzoate (1.0 g kg-1, potassium sorbate (0.3 g kg-1, Lactobacillus plantarum (1 x 10(6 cfu g-1, and Lactobacillus buchneri (3.64 x 10(5 cfu g-1. At the 94th day after ensilage, ethanol concentration was lower in urea, benzoate, sorbate and L. buchneri supplemented silages and higher in L. plantarum inoculated silage, as compared to control. Urea and benzoate treated silages showed the highest and L. plantarum treated silage the lowest in vitro dry matter digestibility. Effluent production was higher in the urea treated silage. Inoculation with L. buchneri reduced 50% ethanol production as compared to control. Urea and L. buchneri reduced yeast count. Aerobic stability was enhanced by L. buchneri and benzoate. Sodium benzoate and L. buchneri were the most promising additives, improving both silage fermentation and aerobic stability; inoculants containing L. plantarum can be deleterious to fermentation and conservation of sugarcane silages.A utilização de silagem de cana-de-açúcar (Saccharum officinarum L. está aumentando no Brasil, mas a intensa produção de etanol durante a fermentação reduz a qualidade da forragem. Este experimento visou avaliar os efeitos de aditivos na fermentação e estabilidade aeróbia de silagens de cana-de-açúcar produzidas em minisilos. Os tratamentos foram (base matéria verde: silagem sem tratamento (controle, uréia (5,0 g kg-1, benzoato de sódio (1,0 g kg-1, sorbato de potássio (0,3 g kg-1, Lactobacillus plantarum (1 x 10(6 ufc g-1 e Lactobacillus buchneri (3,64 x 10(5 ufc g-1. Aos 94 dias após a ensilagem, a concentração de

  18. Demanda de mano de obra y rentabilidad de bancos forrajeros en Esparza, Costa Rica Labor demand and profitability of fodder banks in Esparza, Costa Rica

    Directory of Open Access Journals (Sweden)

    Luz Yaneth Sánchez Sarmiento

    2010-07-01

    Full Text Available En Esparza (Costa Rica se evaluó la viabilidad de invertir en bancos forrajeros (BF como alternativa de suplementación para vacas en sistema doble propósito durante la época seca (125 días. Se usaron como indicadores de rentabilidad el Valor Presente Neto (VPN y la Tasa Interna de Retorno (TIR. Se realizó un análisis de sensibilidad para el incremento del precio de la leche, los concentrados, la gallinaza y la mano de obra. Los modelos se elaboraron comparando los BF de plantas leñosas perennes de corte y acarreo de Cratylia argentea + caña de azúcar (Saccharum officinarum (BFC y de ramoneo Leucaena leucocephala (BFL vs. suplementos comerciales tipos concentrado y gallinaza. En los BFC se requirieron 71.1 jornales/año y el análisis financiero indicó que son rentables al compararlos con la gallinaza (VPN = US$362.2, TIR = 17% y concentrados (VPN = US$1953.9; TIR=39%. En BFL se requirieron 13 jornales/año y fueron igualmente rentables en comparación con gallinaza (VPN = US$1594.7; TIR = 21% y con concentrados (VPN = US$3619.6; TIR=35%. Los modelos fueron sensibles a la producción y cambios de precios en la leche, gallinaza, concentrados y el valor del jornal. Los BF son una buena alternativa para la suplementación de ganado en la época seca y una importante fuente de empleo en la región; sin embargo, los altos costos de la mano de obra contratada pueden limitar la adopción.We made a financial analysis in order to assess the feasibility to invest in Fodder Banks as an alternative for supplementing animals in dual purpose systems during the dry season (125 days. Models were constructed to compare cutting and carrying of perennial woody fodder banks (Veranera Cratylia argentea + sugar cane (Saccharum officinarum and browsing fodder banks of Leucaena leucocephala with two supplements: concentrates and chicken manure. The indicators used for the financial evaluation of the systems were Net Present Value (NPV and Internal Return Rate

  19. Efeitos da aplicação de vinhaça sobre a população e controle químico de plantas daninhas na cultura da cana-de-açucar (Saccharum SPP. Effects of vinasse application on the population and chemical control of weeds in the sugarcane (Saccharum spp.

    Directory of Open Access Journals (Sweden)

    P.J. Christoffoleti

    1985-12-01

    Full Text Available Para avaliar o controle químico e a influência na população de plantas daninhas incidentes na cultura da cana-de-açucar (cana-soca, 3o corte, variedade NA56-79, em função da aplicação de diferentes doses de vinhaça, foi instalado um ensaio em solo pertencente a Usina Santa Lúcia de Açúcar e Álcool, do município de Araras-SP. O solo foi um Latassol Vermelho Amarelo distrófico, textura média, Haplorthox. O experimento foi instalado no dia 10/08/83, com o solo seco no momento da aplicação da vinhaça, não ocorrendo qualquer precipitação nos dez dias seguintes à aplicação. A aplicação da vinhaça foi feita através de veículos tanque, e a aplicação regulada para uma vazão de 50m3/ha , de tal forma que nas dosagens de 100 e 150m3 /ha foram feitas 2 e 3 passadas, respectivamente. Os herbicidas foram aplicados através de pulverizador costal à pressão constante, com um consumo de calda de 370 1/ha. O delineamento experimental foi de blocos casualizados com parcelas subdivididas e 3 repetições e os tratamentos foram vinhaça a 0,50, 100 e 150 m3 /ha e adubação mineral. Por outro lado os subtratamentos foram os herbicidas das alachlor (2-cloro-2-6-dietil-metoximetil-acetanilida a 2,40 kg i.a/ha, diuron (3-(3,4-dicloro-fenil-1, 1-dimetil-uréia a 1,6 kg i.a/há, ametrin (2-(etilamino-4-(isopropilano-6-(metiltio-S-triazina a 2,40 kg i.a/ha e tebuthiuron (N-(5-(1,1-dimetiletil-1, 3,4-tiadiazol-2-il-1,3-N, N-dimetil-uréia a 0,96 kg i.a/ha. A infestação do capim-colchão (Digitaria horizontalis Willd foi maior na área que recebeu apenas adubação mineral, com doses crescentes da vinhaça a população dessa espécie foi se elevando. O controle mais eficiente foi proporcionado por tebuthiuron. Com alachlor houve melhora pronunciada de controle, quando aplicado com 100 m3 /ha de vinhaça. Este fato também ocorreu com o diuron e menos acentuadamente com o ametrin. A tiririca (Cyperus rotundus L. infestou menos intensamente os tratamentos que receberam 150 m3 /ha de vinhaça e aumentou sua população com a diminuição da dose. O melhor controle foi obtido com alachlor e o menos satisfatório com o tebuthiuron. A beldroega (Portulaca oleracea L., guanxuma (Sida rhombifolia L. e falsa-serralha (Emilia sonchifolia D.C. tiveram suas populações alteradas em função da interação entre doses de vinhaça e herbicidas. Verificou-se que as diferentes doses de vinhaça influenciaram a população de capim-colchão, tiririca, beldroega, guanxuma e falsa-serralha. As doses de 100 e 150 m3 /ha, exerceram influência sobre o comportamento dos herbicidas, em especial alachlor e diuron, nas condições do presente experimento.To evaluate the chemical control and the influence of weed population occurring in the sugarcane culture (3rd ratoon, variety NA56-79, with different vinasse dosages, an experiment was installed at Santa Lúcia Sugar and Alcohol Mill, in Araras-SP. The soil was distrophic Red Yellow Latossol, loam texture, Haplorthox. No rainfall level occurred during the 10 days following herbicide application . The experiment was installed in 10/08/83, being the soil dry at the moment of vinasse application, which was through tank truck, with pump discharge without pressure. Herbicides were applied with knapsac at constant pressure CO2 , using Teejet 110.04 nozzle, at an outflow of 370 1/ha. Experimental design was constituted by randomized blocks with split plots and 3 replications. Treatments were 0, 50, 100 and 150 m3/ha vinasse and mineral fertilization. Subtreatments were constituted by the herbicides alachlor at 2.40 kg a.i/ha; diuron at 1.60 kg a.i/ha; ametrin at 2.40 a.i/ha and tebuthiuron at 0.96 kg a.i/ha. Infestation of crabgrass (Digitaria horizontalis Willd was highter in the areas which received only mineral fertilization, however, in the treatments receiving increasing vinasse dosages, crabgrass population was higher with the increase of doses. The most efficient control was provided by tebuthiuron, having alachlor caused a marked control when applied with 150 m3 /ha of vinasse. This was also observed with diuron and less markedly with ametrin. The purple nutsedge (Cyperus rotundus L. had a less intense infestation in the treatments receiving 100 m3 /ha vinasse, increasing its population with lower doses. The best control was achieved was with alchlor and less satisfactory with tebuthiuron. Purslane (Portulaca oleracea L. wire weed (Sida rhombifolia L. and red tassel flower (Emilia sonchifolia D.C. had their populations altered by the interaction of vinasse and the herbicide dosages. Therefore, by this assay it was concluded that the different vinasse dosages influenced the populations of crabgrass, purple nutsedge, purslane, wire weed and red tassel flower. Besides, mainly in the doses of 100 and 150 m3 /ha, it has an influence on the herbicides, specially on alachlor and diuron, under the conditions this experiment was carried out.

  20. Efeitos do período e volume de aplicação na segurança dos tratoristas aplicando herbicidas na cultura de cana-de-açúcar (Saccharum spp. Effects of herbicide spraying period and volume on the safety of tractor drivers spraying herbicides on sugarcane (Saccharum spp. crop

    Directory of Open Access Journals (Sweden)

    J.C. Momesso

    2003-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos do período e volume de aplicação na segurança da atividade de tratoristas aplicando herbicidas na cultura de cana-de-açúcar com o pulverizador de barra montado em trator e a eficiência de equipamentos de proteção individual (EPIs e de uma cabina acoplada ao trator. As exposições dérmicas de 13 condições de trabalho foram avaliadas e analisadas estatisticamente por meio do delineamento inteiramente ao acaso e do esquema fatorial 3 x 2 x 2 + 1. O fator A foi a condição de exposição: 1 exposição dérmica potencial (EDP - sem nenhuma medida de segurança; 2 exposição com cabina no trator (Cabina; e 3 exposição com as vestimentas (EPI. O fator B foi o volume de aplicação: 1 200 L ha-1 e 2 100 L ha-1; e o fator C foi o período de aplicação: 1 diurno e 2 noturno. Como testemunha foi avaliada a EDP do tratorista aplicando na atividade usual de 300 L de calda ha-1, no período diurno. As exposições dérmicas (EDs aos herbicidas considerados nessas condições de trabalho foram estimadas por meio de dados substitutos das EDs avaliadas ao cátion Cu+2 adicionado como traçador nas caldas aplicadas. O pulverizador utilizado foi do modelo PJ 600, com barra de 12 m de comprimento e 24 bicos de jato plano TT 110 04 ou TT 110 02. As 13 condições de trabalho avaliadas foram classificadas como seguras (MS>1 para o tratorista aplicando os herbicidas glyphosate (48% i.a., MSMA (48%, diuron (46,8% + hexazinone (13,2%, clomazone (50%, sulfentrazone (50%, ametryne (50%, diuron (50%, isoxaflutole (75%, metribuzin (48%, 2,4-D (80,6%, ametryne (30% + clomazone (20%, ametryne (73,25% + trifloxysulfuron (1,85% e tebuthiuron (80 % e inseguras para o herbicida atrazine (50%, nos dois períodos e nos três volumes de aplicação, e ametryne (50%, na aplicação diurna e 100 L de calda ha-1. As aplicações noturnas e os volumes de aplicação reduzidos tornaram as condições de trabalho mais seguras, exceto para o atrazine. A eficiência dos EPIs para a aplicação de 300 L ha-1 variou de 69,5 a 89,3%, e a da cabina do trator variou entre 76,4 e 83,3%, em relação aos volumes reduzidos de 100 e 200 L ha-1.This work aimed to evaluate the effects of herbicide application period and volume on the safety of tractor drivers applying herbicide on sugar cane crops using a boom sprayer set and the efficiency of individual protective equipment (IPE, and of a cabin coupled to the tractor. Dermal exposure under thirteen working conditions was evaluated and statistically analyzed in a randomized complete design and a 3 x 2 x 2 + 1 factorial scheme. Factor A constituted the exposure conditions: 1 potential dermal exposure (PDE - without safety measures; 2 tractor cabin exposure (Cabin and 3 exposure with clothes on. Factor B constituted volume applications: 1 200 L ha-1 and 2 100 L ha-1 and factor C constituted the periods of application: 1 day and 2 night. As control, the tractor driver's PDE was evaluated by applying 300 L ha-1 during the day. Dermal exposure (DE to the herbicides utilized under these working conditions was calculated by substituted data of the SEs evaluated to the cation Cu+2 added to the mixture, as a tracer. The sprayer was a PJ 600 model, with 12 m long bar and 24 Teejet TT 110 04 or TT 110 02 nozzles. The thirteen working conditions evaluated were classified as safe to the tractor driver applying the herbicides glyphosate (48% ia, MSMA (48%, diuron (46.8% + hexazinone (13.2%, clomazone (50%, sulfentrazone (50%, ametryne (50%, diuron (50%, isoxaflutole (75%, metribuzin (48%, 2,4 D (80.6%, ametryne (30% + clomazone (20%, ametryne (73.25% + trifloxysulfuron (1.85%, tebuthiuron (80 % and as not safe, applying the atrazine herbicide (50% during the day, and 100 L ha-1. Night applications and reduced application volumes provided safer work conditions , except for atrazine. IPE efficiency for the 300 L ha-1 applications was around 69.5% and 89.3% and 76.4% to 83.3% for the tractor cabin, compared to the reduced volumes of 100 and 200 L ha-1 .

  1. Physiological, morphological and allocational plasticity in understory deciduous trees: importance of plant size and light availability.

    Science.gov (United States)

    Delagrange, Sylvain; Messier, Christian; Lechowicz, Martin J; Dizengremel, Pierre

    2004-07-01

    In a 4-year study, we investigated changes in leaf physiology, crown morphology and whole-tree biomass allocation in seedlings and saplings of shade-tolerant sugar maple (Acer saccharum Marsh.) and intermediate shade-tolerant yellow birch (Betula alleghaniensis Britt.) growing in natural understory light (0.5 to 35% of full sunlight) or in understory light reduced by 50% with shade nets to simulate the effect of gap closure. Leaf physiological parameters were mainly influenced by the light gradient, whereas crown morphological and whole-tree allocational parameters were mainly influenced by tree size. No single physiological, morphological or allocational trait was identified that could explain the difference in shade tolerance between the species. Yellow birch had higher growth rates, biomass allocation to branches and leaf physiological plasticity and lower crown morphological plasticity in unmodified understory light than sugar maple. Sugar maple did not display significant physiological plasticity, but showed variation with tree size in both crown morphology and whole-tree biomass allocation. When sugar maple was small, a greater proportion of whole-tree biomass was allocated to roots. However, physiological differences between the species decreased with decreasing light and most morphological and allocational differences tended to disappear with increasing tree size, suggesting that many species differences in shade-tolerance are expressed mainly during the seedling stage. Understory trees of both species survived for 4 years under shade nets, possibly because of higher plasticity when small and the use of stored reserves when taller. Copyright 2004 Heron Publishing

  2. Yield accumulation in irrigated sugarcane. II. Utilization of intercepted radiation

    International Nuclear Information System (INIS)

    Muchow, R.C.; Evensen, C.I.; Osgood, R.V.; Robertson, M.J.

    1997-01-01

    Intercepted radiation is a major driving variable of crop production under high-input irrigated conditions. Quantitative information on the utilization of radiation in yield accumulation allows extrapolation beyond the current season and location, and when this information is incorporated into crop growth simulation models, the effect of crop age on the productivity of different cultivars can be examined under different climatic conditions. This paper examines the differential performance of high-yielding sugarcane (Saccharum spp. hybrids) crops in terms of the amount of short-wave solar radiation intercepted (Si) and the efficiency of use of intercepted radiation (RUE) in biomass production. Biomass accumulation during the 12- to 24-mo crop cycle was examined for two experiments conducted in Hawaii, and three experiments conducted in tropical Australia from 1991 to 1993. The analysis showed that (i) RUE was much less for growth after 12 mo than in the first 12 mo; (ii) maximum RUE of sugarcane approaches 2.0 g MJ(-1); (iii) biomass accumulation beyond 12 mo was not related directly to radiation utilization; and (iv) cultivars differed in Si, but differences in RUE could not be unequivocally assessed due to the confounding effect of variable recovery of trash in biomass estimates. It is concluded that stalk death and consequent biomass loss are important factors contributing to yield variation in sugarcane crops growing for 12 to 24 mo, with a yield plateau occurring at variable crop ages during the second year of growth

  3. Potassium nutrition in the first and second ratoon sugarcane grown in an Oxisol by a conservationist system

    Directory of Open Access Journals (Sweden)

    Rilner Alves Flores

    2014-03-01

    Full Text Available The presence of mulch on the soil surface after the mechanical harvesting of sugarcane (Saccharum officinarum L. enhances the cycling of nutrients, especially K, which can decrease K fertilizer recommendations for the crop. The aim of this study was to evaluate the effect of K addition to an Oxisol, with an initial concentration 0.07 cmol c K kg-1, in first and second ratoon (no-till sugarcane cultures by a conservationist system, i.e. rational use of fertilizers, use of alternative inputs and especially the maintenance of residues in soil that was previously burned to facilitate cutting. The following K doses were tested: 0, 32.5, 65, 130, and 195 kg K2O ha-1, arranged in a randomized block design with five replicates. Potassium content in the soil and in the plant, as well as the yield and the quality of stalks were evaluated. Soil K application increased K concentration in soil and plant, and was reflected in the production of stalks, with higher production (87.5 and 107.5 t ha-1 with the use of 120 and 123 kg K2O ha-1 in first and second ratoon sugarcane, respectively. At the first 2 yr it was not possible to reduce the K fertilization in ratoon. Therefore, with the introduction of the conservationist system there was an increase (20 t ha-1 at the second ratoon regarding the first one with the same applied rate.

  4. Sistema radicular e produtividade de soqueiras de cana-de-açúcar sob diferentes quantidades de palhada

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    Gisele Silva de Aquino

    2015-12-01

    Full Text Available Resumo: O objetivo deste trabalho foi avaliar o efeito de diferentes quantidades de palhada sobre o sistema radicular e a produtividade da cana-de-açúcar (Saccharum officinarum, de primeira e segunda soqueiras, em Latossolo Vermelho eutroférrico. Seis tratamentos foram avaliados : 0, 5 (25%, 10 (50%, 15 (75% e 20 Mg ha-1 (100% de palhada e cana-queimada, na primeira e segunda soqueiras. Utilizou-se a cana-de-açúcar 'SP80-1816'. Avaliou-se a massa de raízes a 0,45 e 0,75 m de distância da linha de plantio, até 0,60 m de profundidade. Na primeira soqueira, os tratamentos com 50, 75 e 100% de palhada proporcionaram maior massa radicular até 0,20 m de profundidade, em comparação aos tratamentos cana-queimada, 0 e 5 Mg ha-1 (25% de palhada. Os tratamentos com 50 e 75% de palhada proporcionaram produtividades 43% maiores do que a da cana-queimada (110 Mg ha-1. O tratamento com 10 Mg ha-1 (50% foi suficiente para proporcionar aumento da massa radicular e da produtividade da cana-de-açúcar. O sistema radicular e a produtividade da cana-de-açúcar são favorecidos pela mudança do sistema de colheita de cana-queimada para o de cana sob palhada.

  5. Soil and crop residue CO2-C emission under tillage systems in sugarcane-producing areas of southern Brazil

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    Luís Gustavo Teixeira

    2013-10-01

    Full Text Available Appropriate management of agricultural crop residues could result in increases on soil organic carbon (SOC and help to mitigate gas effect. To distinguish the contributions of SOC and sugarcane (Saccharum spp. residues to the short-term CO2-C loss, we studied the influence of several tillage systems: heavy offset disk harrow (HO, chisel plow (CP, rotary tiller (RT, and sugarcane mill tiller (SM in 2008, and CP, RT, SM, moldboard (MP, and subsoiler (SUB in 2009, with and without sugarcane residues relative to no-till (NT in the sugarcane producing region of Brazil. Soil CO2-C emissions were measured daily for two weeks after tillage using portable soil respiration systems. Daily CO2-C emissions declined after tillage regardless of tillage system. In 2008, total CO2-C from SOC and/or residue decomposition was greater for RT and lowest for CP. In 2009, emission was greatest for MP and CP with residues, and smallest for NT. SOC and residue contributed 47 % and 41 %, respectively, to total CO2-C emissions. Regarding the estimated emissions from sugarcane residue and SOC decomposition within the measurement period, CO2-C factor was similar to sugarcane residue and soil organic carbon decomposition, depending on the tillage system applied. Our approach may define new emission factors that are associated to tillage operations on bare or sugarcane-residue-covered soils to estimate the total carbon loss.

  6. 15N-labeled nitrogen from green manure and ammonium sulfate utilization by the sugarcane ratoon

    International Nuclear Information System (INIS)

    Ambrosano, Edmilson Jose; Rossi, Fabricio; Trivelin, Paulo Cesar Ocheuze; Cantarella, Heitor; Ambrosano, Glaucia Maria Bovi; Schammass, Eliana Aparecida; Muraoka, Takashi

    2011-01-01

    Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15 N tracer technique. N was added at the rate of 196 and 70 kg ha -1 as 15 N-labeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (I) Control; (II) AS 15 N; (III) SH 15 N + AS; (IV) SH 15 N; and (V) AS 15 N + SH. Sugarcane was cultivated for five years and was harvested three times. 15 N recovery was evaluated in the two first harvests. In the sum of the three harvests, the highest stalk yields were obtained with a combination of green manure and inorganic N fertilizer; however, in the second cutting the yields were higher where SH was used than in plots with AS. The recovery of N by the first two consecutive harvests accounted for 19 to 21% of the N applied as leguminous green manure and 46 to 49% of the N applied as AS. The amounts of inorganic N, derived from both N sources, present in the 0-0.4 m layer of soil in the first season after N application and were below 1 kg ha -1 . (author)

  7. Residual biomass potential of commercial and pre-commercial sugarcane cultivars

    Directory of Open Access Journals (Sweden)

    Marcos Guimarães de Andrade Landell

    2013-10-01

    Full Text Available Sugarcane (Saccharum spp. is an efficient and sustainable alternative for energy generation compared to non-renewable sources. Currently, during the mechanized harvest process, the straw left in the field can be used in part for the second generation ethanol and increasing the electric energy production. Thus, this study aimed to provide information on the potential for residual biomass cultivars of sugarcane cropping system. This study provides the following information: yield of straw, depending on the calculated leaf area index and the number of tillers per linear meter; primary energy production of several sugarcane genotypes; contribution of dry tops and leaves; biomass yield; and evaluation of fiber, cellulose, hemicellulose and lignin. Preliminary results obtained by researchers of the State of São Paulo, Brazil, and reCviews related studies are presented. The results suggest that the production of sugarcane straw content varies according to the cultivars; the greater mass of sugarcane straw is in the top leaves and that the potential for the crude energy production of sugarcane per area unit can be increased using fiber-rich species or species that produce more straw. The straw indexes was shown to be a good indicator and allow the estimation of straw volumes generated in a sugarcane crop. The cellulose, hemicellulose and lignin composition in sugarcane is distinct among varieties. Therefore, it is possible to develop distinct biomass materials for energy production and for the development of sugarcane mills using biochemical processes and thermal routes.

  8. Restoring ecosystem services to littoral zones of rivers in the urban core of Chongqing, China.

    Science.gov (United States)

    Xian, Xu-Dong; Feng, Yi-Long; Willison, J H Martin; Ai, Li-Jiao; Wang, Ping; Wu, Zhi-Neng

    2015-08-01

    Two examples of the creation of naturalized areas in the littoral zone of the Three Gorges Reservoir in the urban core of Chongqing City, China, are described. The areas were created for the purpose of restoring ecological functions and services. Plants were selected based on surveys of natural wetland vegetation in the region, and experiments were conducted to discover the capacity of species of interest to survive the sometimes extreme hydrological regimes at the sites. Novel methods were developed to stabilize the plants against the rigors of extreme summer floods and constant swash, notably zigzag berms of rocks wrapped in iron mesh. The areas include native reeds, grasses, shrubs, and trees. Plant communities in the areas are zoned according to flooding stress, and their structure is less stable at lower elevations that are subjected to greater stress. The tall grass Saccharum spontaneum (widespread in Southern Asia) and the tree Pterocarya stenoptera (native to Southwest China) are notable for their utility at these sites in the center of a large city. Communities of tall reeds and grasses have become so dense and stable that they now provide the ecosystem services of capturing river sediments and resisting erosion of the river banks. It is recommended that extensive greening of the riparian zones in urban areas of the Three Gorges Reservoir be conducted for the purpose of providing ecosystem services, based in part on the experiences described here.

  9. Effect of sugarcane waste in the control of interrill erosion

    Directory of Open Access Journals (Sweden)

    Wander Cardoso Valim

    2016-06-01

    Full Text Available The cultivation of sugarcane uses different cropping systems that result in varying quantities of crop waste, this may influence soil erosion. The objective of this study was to evaluate the loss of soil and water, the infiltration rate, and soil surface roughness in an area cultivated with sugarcane (Saccharum spp.. Six treatments with different levels of plant waste were evaluated: sugarcane without plant waste; sugarcane with 4.0 Mg ha-1 of waste; sugarcane with 8.0 Mg ha-1 of waste; sugarcane with 12.0 Mg ha-1 of waste; sugarcane with 16.0 Mg ha-1 of waste; and burned sugarcane. The treatments were arranged in a randomized block design with four replications, totalling 24 experimental plots. As soil depth increased, there is reduction in macroporosity, total soil porosity, organic carbon content, mean geometric diameter and weighted mean diameter of the soil aggregates, whereas the bulk density of the soil displays the opposite trend. The presence of sugarcane waste on the soil surface increases the time required for the initiation of surface runoff. Sugarcane waste does not alter soil surface roughness, and at the minimum amount of waste administered (4 Mg ha-1 reduces losses of soil and water and increases the infiltration rate. The lack of soil surface coverage after harvesting the sugarcane contributes to soil and water loss, and reduces the rate of stable infiltration of water into the soil.

  10. Soil solution and sugar maple response to NH(4)NO (3) additions in a base-poor northern hardwood forest of Québec, Canada.

    Science.gov (United States)

    Moore, Jean-David; Houle, Daniel

    2009-08-01

    Nitrogen additions (NH4NO3) at rates of three- and ten-fold ambient atmospheric deposition (8.5 kg ha(-1) year(-1)) were realised in an acid- and base-poor northern hardwood forest of Québec, Canada. Soil solution chemistry, foliar chemistry, crown dieback and basal area growth of sugar maple (Acer saccharum Marsh.) were measured. Except for a transitory increase of NO3 and NH4 concentrations, there was no persistent increase in their level in soil solution 3 years after N treatments, with the exception of one plot out of three, that received the highest N addition, beginning to show persistent and high NO3 concentrations after 2 years of N additions. Three years of N additions have significantly increased the N DRIS index of sugar maple but not N foliar concentration. Potassium, Ca and Mn foliar concentrations, as well as P and Ca DRIS indices, decreased in treated plots after 3 years. No treatment effect was observed for basal area growth and dieback rate. One unexpected result was the significant decrease in foliar Ca even in the treated plots that received low N rates, despite the absence of significant NO3-induced leaching of Ca. The mechanism responsible for the decrease in foliar Ca is not known. Our results, however, clearly demonstrate that increased N deposition at sites with low base saturation may affect Ca nutrition even when clear signs of N saturation are not observed.

  11. Rhizosphere C flux from tree roots to soil: spatial and temporal differences between sugar maple and yellow birch saplings

    Science.gov (United States)

    Phillips, R. P.; Fahey, T. J.

    2003-12-01

    Rhizosphere carbon flux (RCF) has rarely been measured for intact root-soil systems. We measured RCF for eight year-old saplings of sugar maple (Acer saccharum) and yellow birch (Betula allegheniensis) collected from Hubbard Brook Experimental Forest and transplanted into 35 cm diameter pots with native soil horizons intact. We hypothesized birch roots which support ectomycorrhizal fungi would release more C to the rhizosphere than sugar maple roots which support vesicular-arbuscular mycorrhizal fungi. Saplings (n=5) were pulse-labeled with 13CO2 at ambient CO2 concentrations for 4-6 hours, and the label was chased through rhizosphere and bulk soil pools in organic and mineral horizons for 7 days. We observed immediate appearance of the label in rhizosphere soil, and there was a striking difference in the temporal pattern of 13C concentration between species. In maple, peak concentration of the label appeared at day 1 and declined over time whereas in birch the label increased in concentration over the 7 day chase period. As a result, total RCF was 2-3 times greater from birch roots. We estimate at least 5% and 10% of NPP may be released from this flux pathway in sugar maple and yellow birch saplings respectively. These results suggest that rhizosphere C flux likely represents a substantial proportion of NPP in northern hardwood forests, and may be influenced by trees species and mycorrhizal association.

  12. {sup 15}N-labeled nitrogen from green manure and ammonium sulfate utilization by the sugarcane ratoon

    Energy Technology Data Exchange (ETDEWEB)

    Ambrosano, Edmilson Jose; Rossi, Fabricio, E-mail: ambrosano@apta.sp.gov.b [Agencia Paulista de Tecnologia dos Agronegocios (APTA), Piracicapa, SP (Brazil). Polo Rigional Centro Sul; Trivelin, Paulo Cesar Ocheuze [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Isotopos Estaveis; Cantarella, Heitor [Agencia Paulista de Tecnologia dos Agronegocios (APTA/IAC), Campinas, SP (Brazil). Instituto Agronomico de Campinas. Centro de Solos e Recursos Agroambientais; Ambrosano, Glaucia Maria Bovi [Universidade de Campinas (UNICAMP/FOP), Piracicaba, SP (Brazil). Fac. de Odontologia de Piracicaba. Dept. de Odontologia Social, Bioestatistica; Schammass, Eliana Aparecida [Agencia Paulista de Tecnologia dos Agronegocios (APTA/IZ), Nova Odessa, SP (Brazil). Instituto de Zootecnia; Muraoka, Takashi [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Fertilidade do solo

    2011-05-15

    Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the {sup 15}N tracer technique. N was added at the rate of 196 and 70 kg ha{sup -1} as {sup 15}N-labeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (I) Control; (II) AS{sup 15}N; (III) SH{sup 15}N + AS; (IV) SH{sup 15}N; and (V) AS{sup 15}N + SH. Sugarcane was cultivated for five years and was harvested three times. {sup 15}N recovery was evaluated in the two first harvests. In the sum of the three harvests, the highest stalk yields were obtained with a combination of green manure and inorganic N fertilizer; however, in the second cutting the yields were higher where SH was used than in plots with AS. The recovery of N by the first two consecutive harvests accounted for 19 to 21% of the N applied as leguminous green manure and 46 to 49% of the N applied as AS. The amounts of inorganic N, derived from both N sources, present in the 0-0.4 m layer of soil in the first season after N application and were below 1 kg ha{sup -1}. (author)

  13. Effects of elevated ozone on CO2 uptake and leaf structure in sugar maple under two light environments

    International Nuclear Information System (INIS)

    Bäck, J.; Vanderklein, D.W.; Topa, M.A.

    1999-01-01

    The interactive effects of ozone and light on leaf structure, carbon dioxide uptake and short-term carbon allocation of sugar maple (Acer saccharum Marsh.) seedlings were examined using gas exchange measurements and 14 C-macroautoradiographic techniques. Two-year-old sugar maple seedlings were fumigated from budbreak for 5 months with ambient or 3 × ambient ozone in open-top chambers, receiving either 35% (high light) or 15% (low light) of full sunlight. Ozone accelerated leaf senescence, and reduced net photosynthesis, 14 CO 2 uptake and stomatal conductance, with the effects being most pronounced under low light. The proportion of intercellular space increased in leaves of seedlings grown under elevated ozone and low light, possibly enhancing the susceptibility of mesophyll cells to ozone by increasing the cumulative dose per mesophyll cell. Indeed, damage to spongy mesophyll cells in the elevated ozone × low light treatment was especially frequent. 14 C macroautoradioraphy revealed heterogeneous uptake of 14 CO 2 in well defined areole regions, suggesting patchy stomatal behaviour in all treatments. However, in seedlings grown under elevated ozone and low light, the highest 14 CO 2 uptake occurred along larger veins, while interveinal regions exhibited little or no uptake. Although visible symptoms of ozone injury were not apparent in these seedlings, the cellular damage, reduced photosynthetic rates and reduced whole-leaf chlorophyll levels corroborate the visual scaling of whole-plant senescence, suggesting that the ozone × low light treatment accelerated senescence or senescence-like injury in sugar maple. (author)

  14. Isolation, Identification, and Biological Evaluation of Phenolic Compounds from a Traditional North American Confectionery, Maple Sugar.

    Science.gov (United States)

    Liu, Yongqiang; Rose, Kenneth N; DaSilva, Nicholas A; Johnson, Shelby L; Seeram, Navindra P

    2017-05-31

    Maple sap, collected from the sugar maple (Acer saccharum) tree, is boiled to produce the popular plant-derived sweetener, maple syrup, which can then be further evaporated to yield a traditional North American confectionery, maple sugar. Although maple sap and maple syrup have been previously studied, the phytochemical constituents of maple sugar are unknown. Herein, 30 phenolic compounds, 1-30, primarily lignans, were isolated and identified (by HRESIMS and NMR) from maple sugar. The isolates included the phenylpropanoid-based lignan tetramers (erythro,erythro)-4″,4‴-dihydroxy-3,3',3″,3‴,5,5'-hexamethoxy-7,9';7',9-diepoxy-4,8″;4',8‴-bisoxy-8,8'-dineolignan-7″,7‴,9″,9‴-tetraol, 29, and (threo,erythro)-4″,4‴-dihydroxy-3,3',3″,3‴,5,5'-hexamethoxy-7,9';7',9-diepoxy-4,8″;4',8‴-bisoxy-8,8'-dineolignan-7″,7‴,9″,9‴-tetraol, 30, neither of which have been identified from maple sap or maple syrup before. Twenty of the isolates (selected on the basis of sample quantity available) were evaluated for their potential biological effects against lipopolysaccharide-induced inflammation in BV-2 microglia in vitro and juglone-induced oxidative stress in Caenorhabditis elegans in vivo. The current study increases scientific knowledge of possible bioactive compounds present in maple-derived foods including maple sugar.

  15. Calcium and aluminum impacts on sugar maple physiology in a northern hardwood forest.

    Science.gov (United States)

    Halman, Joshua M; Schaberg, Paul G; Hawley, Gary J; Pardo, Linda H; Fahey, Timothy J

    2013-11-01

    Forests of northeastern North America have been exposed to anthropogenic acidic inputs for decades, resulting in altered cation relations and disruptions to associated physiological processes in multiple tree species, including sugar maple (Acer saccharum Marsh.). In the current study, the impacts of calcium (Ca) and aluminum (Al) additions on mature sugar maple physiology were evaluated at the Hubbard Brook Experimental Forest (Thornton, NH, USA) to assess remediation (Ca addition) or exacerbation (Al addition) of current acidified conditions. Fine root cation concentrations and membrane integrity, carbon (C) allocation, foliar cation concentrations and antioxidant activity, foliar response to a spring freezing event and reproductive ability (flowering, seed quantity, filled seed and seed germination) were evaluated for dominant sugar maple trees in a replicated plot study. Root damage and foliar antioxidant activity were highest in Al-treated trees, while growth-associated C, foliar re-flush following a spring frost and reproductive ability were highest in Ca-treated trees. In general, we found that trees on Ca-treated plots preferentially used C resources for growth and reproductive processes, whereas Al-treated trees devoted C to defense-based processes. Similarities between Al-treated and control trees were observed for foliar cation concentrations, C partitioning and seed production, suggesting that sugar maples growing in native forests may be more stressed than previously perceived. Our experiment suggests that disruption of the balance of Ca and Al in sugar maples by acid deposition continues to be an important driver of tree health.

  16. DISTRIBUTION AND DIVERSITY OF FUSARIUM SPECIES ASSOCIATED WITH GRASSES IN TEN STATES THROUGHOUT PENINSULAR MALAYSIA

    Directory of Open Access Journals (Sweden)

    NUR AIN IZZATI, M.Z

    2009-01-01

    Full Text Available Fusarium is one of the important genera associated with grasses as saprophytes, endophytes and pathogens. A study was carried out on distribution and diversity of Fusarium species associated with two groups of grasses in 10 states throughout Peninsular Malaysia i.e. agricultural grasses (Oryza sativa and Saccharum officinarum and non-agricultural grasses (Axonopus compressus, Centhotheca lappacea, Chloris barbata, Crysopogon aciculatus, Cyanadon dactylon, Dactyloctenium aegyptium, Digitaria ciliaris, Echinochloa colona, Eleusine indica, Eragrostis amabilis, Eragrostis malayana, Eragrostis uniloides, Ischaemum magnum, Panicum brevifolium, Panicum millaneum, Panicum repens, Paspalum commersonii, Paspalum conjugatum, Paspalum orbiculare, Pennisetum purpureum, Sacciolepis indica, Sporobolus diander and Sporobolus indicus. A total of 474 isolates were single-spored and identified by morphological characteristics. F. semitectum was frequently isolated (23.6%, followed by F. sacchari and F. fujikuroi with 15.4% and 14.6%, respectively. The other nine species were F. solani (10.3%, F. proliferatum (8.9%, F. oxysporum (7.4%, F. subglutinans (6.5%, F. equiseti (5.5%, F. verticillioides (3.4%, F. compactum (2.5%, F. chlamydosporum (1.1% and F. longipes (0.8%. Based on the Shannon-Weiner Index, F. solani was the highest (H' = 2.62 isolated from grasses. Species of Fusarium from O. sativa were widely diverse with 11 species, followed by non-agricultural grasses with nine species and S. officinarum with only six species. This is the first report on diversity of Fusarium associated with grasses in Malaysia.

  17. Sustained by First Nations: European newcomers' use of Indigenous plant foods in temperate North America

    Directory of Open Access Journals (Sweden)

    Nancy J. Turner

    2012-12-01

    Full Text Available Indigenous Peoples of North America have collectively used approximately 1800 different native species of plants, algae, lichens and fungi as food. When European explorers, traders and settlers arrived on the continent, these native foods, often identified and offered by Indigenous hosts, gave them sustenance and in some cases saved them from starvation. Over the years, some of these species – particularly various types of berries, such as blueberries and cranberries (Vaccinium spp., wild raspberries and blackberries (Rubus spp., and wild strawberries (Fragaria spp., and various types of nuts (Corylus spp., Carya spp., Juglans spp., Pinus spp., along with wild-rice (Zizania spp. and maple syrup (from Acer saccharum – became more widely adopted and remain in use to the present day. Some of these and some other species were used in plant breeding programs, as germplasm for hybridization programs, or to strengthen a crop's resistance to disease. At the same time, many nutritious Indigenous foods fell out of use among Indigenous Peoples themselves, and along with their lessened use came a loss of associated knowledge and cultural identity. Today, for a variety of reasons, from improving people's health and regaining their cultural heritage, to enhancing dietary diversity and enjoyment of diverse foods, some of the species that have dwindled in their use have been “rediscovered” by Indigenous and non-Indigenous Peoples, and indications are that their benefits to humanity will continue into the future.

  18. Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane yield

    Energy Technology Data Exchange (ETDEWEB)

    Ambrosano, Edmilson Jose; Rossi, Fabricio; Guirado, Nivaldo; Teramoto, Juliana Rolim Salome [Agencia Paulista de Tecnologia dos Agronegocios (APTA), Piracicaba, SP (Brazil). Polo Regional Centro Sul; Azcon, Rozario [Consejo Superior de Investigaciones Cientificas (CSIC), Granada (Spain). Estacao Experimental de Zaidin; Cantarela, Heitor [Agencia Paulista de Tecnologia dos Agronegocios (APTA/IAC), Campinas, SP (Brazil). Inst. Agronomico. Centro de Solos e Recursos Ambientais; Ambrosano, Glaucia Maria Bovi [Universidade Estadual de Campinas (UNICAMP), Piracicaba, SP (Brazil). Fac. de Odontologia. Dept. de Odontologia Social], Email: ambrosano@apta.sp.gov.br; Schammass, Eliana Aparecida [Agencia Paulista de Tecnologia dos Agronegocios (APTA/IZ), Nova Odessa, SP (Brazil). Inst. de Zootecnia; Muraoka, Takashi; Trivelin, Paulo Cesar Ocheuze [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Ungaro, Maria Regina Goncalves [Agencia Paulista de Tecnologia dos Agronegocios (APTA/IAC), Campinas, SP (Brazil). Inst. Agronomico. Centro de Plantas Graniferas

    2010-07-01

    Sugarcane (Saccharum spp.) is an important crop for sugar production and agro-energy purposes in Brazil. In the sugarcane production system after a 4- to 8-year cycle crop rotation may be used before replanting sugarcane to improve soil conditions and give an extra income. This study had the objective of characterizing the biomass and the natural colonization of arbuscular mycorrhizal fungi (AMF) of leguminous green manure and sunflower (Helianthus annuus L.) in rotation with sugarcane. Their effect on stalk and sugar yield of sugarcane cv. IAC 87-3396 grown subsequently was also studied. Cane yield was harvested in three subsequent cuttings. Peanut cv. IAC-Caiapo, sunflower cv. IAC-Uruguai and velvet bean (Mucuna aterrimum Piper and Tracy) were the rotational crops that resulted in the greater percentage of AMF. Sunflower was the specie that most extracted nutrients from the soil, followed by peanut cv. IAC-Tatu and mung bean (Vigna radiata L. Wilczek). The colonization with AMF had a positive correlation with sugarcane plant height, at the first cut (p = 0.01 and R = 0.52) but not with the stalk or cane yields. Sunflower was the rotational crop that brought about the greatest yield increase of the subsequent sugarcane crop: 46% increase in stalk yield and 50% in sugar yield compared with the control. Except for both peanut varieties, all rotational crops caused an increase in net income of the cropping system in the average of three sugarcane harvests. (author)

  19. Behavior of two phenyl urea herbicides in clayey soils and effect of alternating dry-wet conditions on their availability.

    Science.gov (United States)

    Haouari, Jamila; Dahchour, Abdelmalek; Peña-Heras, Arancha; Louchard, Xzavier; Lennartz, Berndt; Alaoui, Mohamed Elbelghiti; Satrallah, Ahmad

    2006-01-01

    Adsorption and mobility of linuron (3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea) and diuron (3-(3,4-dichlorophenyl)-1, 1-dimethylurea) were studied in clayey soils from the Gharb area (Morocco). Soils A and B were planted with sun flower (Helianthus annuus) while soil C was planted with sugar cane (Saccharum offcinarum). Adsorption was studied for linuron in soils A and B, while mobility was studied only in soil B. Adsorption data were found to fit the Freundlich equation with correlation coefficients r2 > 0.9. Freundlich coefficients (Kf, nf) were in agreement with L and S isotherm types for soils A and B, respectively. Values of Koc (195 and 102) indicate moderate adsorption. Desorption isotherms for linuron showed hysteresis for both soils. The pesticide would be more bound to soil A (H = 8.44) than to soil B (H = 4.01). The effect of alternating wet and dry conditions was tested for soils A and B. Results showed that retention would increase in soil subject to an additional wet and dry cycle. In the case of diuron isotherm was of type L in soil C. Desorption was noticeable at high concentrations and tended to decrease when concentrations diminished. Mobility of linuron was tested in polyvinyle chloride (PVC) columns, which received different treatments before their percolation. The pesticide was more mobile in a previously saturated column. In columns subject to a drying step after saturation with water, linuron mobility was greatly reduced.

  20. Nutritional ecology of the formosan subterranean termite (Isoptera: Rhinotermitidae): feeding response to commercial wood species.

    Science.gov (United States)

    Morales-Ramos, J A; Rojas, M G

    2001-04-01

    The feeding preferences of the Formosan subterranean termite, Coptotermes formosanus Shiraki, were tested in three separate experiments on 28 different wood species. Experiment 1 was a multiple-choice test designed to test relative preferences among 24 wood species commercially available in New Orleans, LA. Experiment 2 was a similar study designed to test relative preferences among 21 wood species shown or reported to be unpalatable to the Formosan subterranean termite. Experiment 3 was a no-choice test to examine the feeding deterrence of the 10 least preferred wood species. Preference was determined by consumption rates. Birch (Betula alleghaniensis Britton), red gum (Liquidambar styraciflua L.), Parana pine [Araucaria angustifolia (Bert.) 1, sugar maple (Acer saccharum Marsh.), pecan (Carya illinoensis Wangenh.), and northern red oak (Quercus rubra L.) were the most preferred species by C. formosanus in order of consumption rate. All of these species were significantly more preferred than southern yellow pine (Pinus taeda L.), widely used for monitoring. Sinker cypress [ = old growth bald cypress, Taxodium distichum (L.)], western red cedar (Thuja plicata Donn), Alaskan yellow cedar (Chamaecyparis nootkatensis D. Don), eastern red cedar (Juniperus virginiana L.), sassafras [Sassafras albidum (Nutt.)], Spanish cedar (Cedrella odorata L.), Honduras mahogany (Swietenia macrophyla King), Indian rosewood (Dalbergia latifolia Roxb.), Honduras rosewood (D. stevensonii Standl.), and morado (Machaerium sp.) induced significant feeding deterrence and mortality to C. formosanus. The last eight species produced 100% mortality after 3 mo.

  1. Sugarcane Elongin C is involved in infection by sugarcane mosaic disease pathogens.

    Science.gov (United States)

    Zhai, Yushan; Deng, Yuqing; Cheng, Guangyuan; Peng, Lei; Zheng, Yanru; Yang, Yongqing; Xu, Jingsheng

    2015-10-23

    Sugarcane (Saccharum sp. hybrid) provides the main source of sugar for humans. Sugarcane mosaic disease (SMD) is a major threat to sugarcane production. Currently, control of SMD is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. Understanding the mechanism of viral infection may facilitate novel strategies to breed cultivars resistant to SMD and to control the disease. In this study, a wide interaction was detected between the viral VPg protein and host proteins. Several genes were screened from sugarcane cDNA library that could interact with Sugarcane streak mosaic virus VPg, including SceIF4E1 and ScELC. ScELC was predicted to be a cytoplasmic protein, but subcellular localization analysis showed it was distributed both in cytoplasmic and nuclear, and interactions were also detected between ScELC and VPg of SCMV or SrMV that reveal ScELC was widely used in the SMD pathogen infection process. ScELC and VPgs interacted in the nucleus, and may function to enhance the viral transcription rate. ScELC also interacted with SceIF4E2 both in the cytoplasm and nucleus, but not with SceIF4E1 and SceIF4E3. These results suggest that ScELC may be essential for the function of SceIF4E2, an isomer of eIF4E. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. An ecological assessment of Hispid Hare Caprolagus hispidus (Mammalia: Lagomorpha: Leporidae in Manas National Park, Assam, India

    Directory of Open Access Journals (Sweden)

    Naba K. Nath

    2015-12-01

    Full Text Available This study of the Hispid Hare Caprolagus hispidus in the tall grassland habitat of Manas National Park, Assam during 2009–2010 is the first detailed assessment in northeastern India.  We assessed the status, distribution, habitat use and key threats to this rare and little studied lagomorph species.  After interviewing local forest staff, 20 grassland patches within a survey area of 2.65ha were selected and transects (50x2 m laid randomly to determine the presence/absence of Hispid Hare by recording pellets and other indirect evidence.  Hare presence was recorded in 17 grassland patches within transects dominated by Imperata cylindrica and Saccharum narenga.  Hispid Hare preferred dry savannah grasslands to wet alluvial grasslands during winter and avoided recently burned patches due to lack of cover and food.  The distribution pattern observed was clumped (s2/a = 6.2, with more evidence of Hispid Hare presence in areas where ground cover was dense, dry and away from water sources. Population density was estimated at 381.55 individuals/km2, which in comparison with other studies indicates that Manas National Park currently holds the highest density of Hispid Hare.  Habitat loss due to overgrazing, unsustainable thatch harvesting, burning of grassland, weed invasion, encroachment and hunting were identified as key threats which must be addressed to ensure survival of this threatened species in the Park.  

  3. Biodiversity and Phytosociological Studies of Upstream and Downstream Riparian Areas of Pakistan: Special Reference to Taunsa Wildlife Sanctuary and Keti Shah Forests

    International Nuclear Information System (INIS)

    Arfeen, R. Z.; Saleem, A.; Mirza, S. N.; Tayyab, H. M.; Akmal, M.; Afzal, O.

    2015-01-01

    Pakistan riparian zone mostly belongs to Sindh and Punjab provinces and prone to climatic problems and anthropogenic activities. The research was conduct to estimate and compare the structure and composition of riverine floral diversity in low riparian zone of River Indus. The data was collected from Keti Shah forest and Taunsa wildlife sanctuary. Total 14259 plants/individuals were recorded, which belong to 54 plant species with 18 different families. In Taunsa pre-monsoon survey, total 30 plant species were found with 4476 plants from 16 different families. In Taunsa post-monsoon survey total 3348 individuals were recorded from 20 plant species and 9 families. Similarly, in Keti Shah forest, total 3975 individual were recorded from 22 species and 11 families during the pre-monsoon season and 2460 plants were recorded in post-monsoon season, belonging to 16 species and 10 families. These species mostly belong to Fabaceae, Poaceae, Cyperaceae and Asclepiadaceae. Different phytosociological parameters indicate Tamarix dioca, Cynodon dactylon, Desmostachya bipinnata, Imperata cylindrica, Fimbristylis hispidula, Acacia nilotica, Phragmites karka, Tamarix sp. and Saccharum bengalense as dominant species. The biodiversity in upstream and downstream areas were rich in pre-monsoon season in comparison to post-monsoon season in surveyed areas. This study is useful for management of the area in the future as conservation strategies can be made through considering the adaptive tree species in future plantation and endangered species can be conserved. (author)

  4. Three-dimensional mapping of light transmittance and foliage distribution using lidar

    International Nuclear Information System (INIS)

    Todd, K.W.; Csillag, F.; Atkinson, P.M.

    2003-01-01

    The horizontal and vertical distributions of light transmittance were evaluated as a function of foliage distribution using lidar (light detection and ranging) observations for a sugar maple (Acer saccharum) stand in the Turkey Lakes Watershed. Along the vertical profile of vegetation, horizontal slices of probability of light transmittance were derived from an Optech ALTM 1225 instrument's return pulses (two discrete, 15-cm diameter returns) using indicator kriging. These predictions were compared with (i) below canopy (1-cm spatial resolution) transect measurements of the fraction of photosynthetically active radiation (FPAR) and (ii) measurements of tree height. A first-order trend was initially removed from the lidar returns. The vertical distribution of vegetation height was then sliced into nine percentiles and indicator variograms were fitted to them. Variogram parameters were found to vary as a function of foliage height above ground. In this paper, we show that the relationship between ground measurements of FPAR and kriged estimates of vegetation cover becomes stronger and tighter at coarser spatial resolutions. Three-dimensional maps of foliage distribution were computed as stacks of the percentile probability surfaces. These probability surfaces showed correspondence with individual tree-based observations and provided a much more detailed characterization of quasi-continuous foliage distribution. These results suggest that discrete-return lidar provides a promising technology to capture variations of foliage characteristics in forests to support the development of functional linkages between biophysical and ecological studies. (author)

  5. Identifikasi Dan Uji Antagonisme Jamur Endofit Tanaman Tebu (Saccharum Officinarum L.) Terhadap Perkembangan Xanthomonas Albilineans L. Dengan Metode Sterilisasi Autoklaf Dan Membran Filter

    OpenAIRE

    Wahyuni, Siti Hardianti

    2016-01-01

    This research was conducted in a Laboratory of Agricuture Faculty and greenhouse of Agricuture Faculty, North Sumatra University, Medan, from April until December 2014. The research used completely randomized design with three factors and three replications. The first factor were endophytic fungi 1, endophytic fungi 2, endophytic fungi 3, endophytic fungi 4, endophytic fungi 5, endophytic fungi 6, endophytic fungi 7, and endophytic fungi 8. The second factor were dilution 10-1, 10-2, 10-3 da...

  6. Indicadores de crecimiento de una población de 48 clones de caña de azúcar (Saccharum spp. con valor forrajero

    Directory of Open Access Journals (Sweden)

    Yoslen Fernández Gálvez

    2016-09-01

    Full Text Available Este trabajo tuvo como objetivo determinar indicadores de crecimiento en una población de 48 clones de caña de azúcar con rasgos fenotípicos promisorios para la producción de forraje. Se evaluaron los indicadores: área foliar (A; índice de área foliar (LAI; razón de área foliar (LAR; área foliar específica (SLA; razón de peso foliar (LWR; tasa de crecimiento del cultivo (CGR; tasa de asimilación neta (NAR; tasa de crecimiento relativo en peso (RGR; velocidad de producción de biomasa (G; duración del área foliar (LAD y duración de la biomasa (Z, con una periodicidad de un mes desde los 187 y hasta los 370 días. Se determinaron los valores mínimos, medios, máximos y la varianza de la población en todas las edades de corte y para cada una de las variables evaluadas. Los resultados obtenidos permiten contar con valores cuantitativos que pueden ser utilizados como referencia para la selección y evaluación de genotipos con características forrajeras para la alimentación de rumiantes.

  7. ISOLASI, KARAKTERISASI, DAN IDENTIFIKASI MIKOFLORA DARI RIZOSFER TANAH PERTANIAN TEBU (SACCHARUM OFFICINARUM L. SEBAGAI BAHAN AJAR KINGDOM FUNGI UNTUK SISWA KELAS X SMA

    Directory of Open Access Journals (Sweden)

    Yesy Maulina Nadhifah

    2016-10-01

    Full Text Available Agricultural area, particularly in the rhizosfer area there are various species of mold which lives mainly in the soil. The students have known molds which live in nature but they have not recognized mold which lives in the soil. It is required to develop a teaching material in the form of module which is created based on a research result. The results of this study were; (1 it has been discovered 7 species of mold, which are Trichoderma harzianum, Penicillium nalgiovense, Penicillium corylophilum, Penicillium funiculosum, Penicillium camemberti, Aspergillus ochraceus, dan Penicillium citrinum, (2 the most dominant mold species was Trichoderma harzianum with the total number 1,8 x 104 cfu for every gram soil sample, and (3 there has been arranged of the learning module about fungi kingdom for students in high school level. Tanah pertanian, khususnya di daerah rizosfer, merupakan habitat dari berbagai spesies kapang yang hidup di dalamnya. Para siswa telah mengenal kapang yang tumbuh di alam tetapi mereka belum mengenal kapang yang hidup di dalam tanah pertanian. Perlu dikembangkan sebuah bahan ajar berupa modul yang dibuat berdasarkan hasil penelitian. Hasil penelitian ini, meliputi (1 ditemukan 7 spesies kapang, yaitu Trichoderma harzianum, Penicillium nalgiovense, Penicillium corylophilum, Penicillium funiculosum, Penicillium camemberti, Aspergillus ochraceus, dan Penicillium citrinum, (2 spesies kapang yang paling dominan adalah Trichoderma harzianum dengan total jumlah 1,8 x 104 cfu/g sampel tanah, dan (3 telah tersusun bahan ajar Biologi berupa modul Kingdom Fungi para siswa SMA.

  8. Studies on the absorption, distribution of rare earth elements and its effect on absorption of phosphorus and potassium in sugarcane (Saccharum officnarum L.)

    International Nuclear Information System (INIS)

    Kuang Yanhua; Liu Qiongying; Zheng Yuemei; Deng Zhouhuo; Xu Shengjie

    1991-01-01

    Study on the absorption, distribution and translocation of area earth elements (RE) and their effects on absorption of phosphorus and potassium ( 86 Rb as tracer) in sugarcane showed that the sugarcane could absorb soluble RE fast by its root or leaves. Within the 1-7 days after treatment, the absorption rate of 141 Ce in organs of the plant increased with the time. At the 7th day after treatment, the absorption rate of 141 Ce by sugarcane plant reached the maximum which was about 2-3 times of that at the 1st day and after that it gradually decreased. The utilization efficiency of 141 Ce by sugarcane plant was 0.836-3.24%. The absorption and distribution of RE in sugarcane plant varied with the treatment methods. It was shown that about 90% 141 Ce in nutrient solution was concentrated in the roots while only about 10% of them was transferred into the shoots. The distribution and accumulation of 141 Ce in suggarcene plant was in order of root>stem>leaf. If smeared on the leaf of sugarcane, the distribution and accumulation of 141 Ce in sugarcane plant was in order of leaf>stem>root. When treated with 300, 500 and 800 ppm R(NO 3 ) 3 ·4H 2 O, Ce, La, Sm, Pr, Nd and Y, the absorption and translocation rate of phosphorus and rubidium in sugarcane were increased

  9. Stem wood properties of Populus tremuloides, Betula papyrifera and Acer saccharum saplings after three years of treatments to elevated carbon dioxide and ozone

    Science.gov (United States)

    Seija Kaakinen; Katri Kostiainen; Fredrik Ek; Pekka Saranpaa; Mark E. Kubiske; Jaak Sober; David F. Karnosky; Elina Vapaavuori

    2004-01-01

    The aim of this study was to examine the effects of elevated carbon dioxide [CO2] and ozone [O3] and their interaction on wood chemistry and anatomy of five clones of 3-year-old trembling aspen (Populus tremuloides Michx.). Wood chemistry was studied also on paper birch (Betula papyrifera...

  10. Studies on silica deposition in sugarcane (Saccharum spp. ) using scanning electron microscopy, energy-dispersive X-ray analysis, neutron activation analysis, and light microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Kaufman, P B; Takeoka, Y; Carlson, T J; Bigelow, W C; Jones, J D; Moore, P H; Ghosheh, N S [Michigan Univ., Ann Arbor (USA)

    1979-06-01

    Marked differences in silicon content in internodes of two sugarcane cultivars as revealed by neutron activation analysis, were closely correlated with number of silica cells per unit area in the epidermal system of the internodes of the two cultivars, as indicated by scanning electron microscopy and X-ray analysis. Light microscopy of epidermal peels showed that silica cells are capable of transmitting significantly more light through themselves than do other types of adjacent epidermal cells. This could be of great significance to total amount of carbon fixed by photosynthesizing mesophyll cells in leaves and cortical cells in internodes below the epidermis, especially in sugarcane cultivars with high densities of silica cells in their shoot epidermal systems. This has led to propose a window hypothesis, which indicates that silica cells in sugarcane, and in other grasses, act like windows in the epidermal system, allowing more light to be transmitted to photosynthetic tissue below than would occur if silica cells were absent.

  11. Bagacillo de caña (Saccharum officinarum predigerido por vapor a presión como alimento básico para novillos de carne

    Directory of Open Access Journals (Sweden)

    Ortiz H. Oscar

    1987-03-01

    Full Text Available Al someter el bagacillo a altas presiones (18.3, 19.0, 19.7 y 20.4 kg/cm2 durante 4, 6, 8 y 10 minutos se incrementó significativamente la digestibilidad "in vitro" y la degradabilidad "in situ "de la materia seca. La aceptabilidad del bagacillo predigerido al vapor-BPOJ (O, 40, 60 y 85.42% fue baja y se presentó disminución en el consumo y pérdidas de peso en novillos cebú que consumieron niveles por encima del 60%. No se observaron diferencias significativas (P < 0.05 en la ganancia de peso promedio de novillos alimentados con BPDV y altos niveles de urea, usando una cantidad restringida de pasto rey (TI, bagazo crudo entero (T2 y melaza de caña (T3; la exclusión de fibra larga (T3 no afectó el desempeño animal. Se detectaron niveles de furfural por debajo del 1 % en el BPIN (base fresca; los análisis espectroscópicos (UV, RI, RMN registraron la presencia de grupos carboxilos e hidroxilos, indicando la probable existencia de fenoles.De-rinded sugar cane bagasse (the pith was subjected to high steam pressures (18.3, 19.0, 19.7 and 20.4 kg/cm2 during 4, 5, 8and 10 minutes. In vitro digestibilities and JD. situ degradabilities were significantly improved by the treatments. The steam pre-digested bagasse pith-SPBP (0,20, 40, 60 y 85.42% acceptability were lowand the results showed very high weight losses of steers teed 60 and 80% SPBP of their diet. No statistical difference (P < 0.05 was observed for average daily gains of steer fed on high levels of SPBP supplemented with high levels of urea, using three treatment: restricted amount of king grass (T1 whole raw bagasse (T2 and cane molasses(T3; the exclusion of the long fiber (T3 did not effect animal performance. Furfural levels below 1% SPBP (fresch basis were detected; spectrometric analysis (UV, RI and FMN registered the presence of carboxil and hidroxil groups indicating the probable existence of phenols.

  12. La inoculación de plantas con Pantoea sp., bacteria solubilizadora de fosfatos, incrementa la concentración de P en los tejidos foliares Plant inoculation with Pantoea sp., phosphate solubilising-bacteria increases P concentration in leaf tissues

    Directory of Open Access Journals (Sweden)

    Ortega-Rodés Patricia

    2008-07-01

    Full Text Available El fósforo (P es un macronutriente mineral esencial para las plantas. Aunque puede encontrarse en los suelos en diferentes formas minerales, la baja solubilidad de estos disminuye su disponibilidad para las plantas, y el nutriente debe aplicarse como fertilizante a los cultivos. Las reservas mundiales de P son limitadas y tendrán una reducción considerable en los próximos años. Usar microorganismos solubilizadores de fosfatos como inoculante para los cultivos es una alternativa biotecnológica para incrementar la disponibilidad del nutriente. Pantoea sp. (cepa 9C es una bacteria endofítica fijadora de nitrógeno, aislada del interior de tallos de la caña de azúcar (Loiret et ál., 2004; este microorganismo produjo halos de solubilización con tamaños de hasta 6 mm en medio sólido NBRI-P en 7 días a 30 ºC, y en ese tiempo y condiciones solubilizó Ca3(PO42 en el medio líquido hasta acumular 1128 μg P mL-1. La bacteria sobrevivió durante 35 días en un sustrato preparado con mezcla de Vermiculita y suelo ferralítico rojo (Cambisol Ferrálico, ródico, alcanzando poblaciones de 3,2 x 1015 células g-1. Plantas de rábano (Raphanus sativus, L. var. Scarlet Globe, de alta demanda de P y crecimiento rápido, usadas como modelo y cultivadas en suelos inoculados con el microorganismo, absorbieron más P que las plantas no inoculadas, alcanzando en los tejidos foliares concentraciones ≥ 3500 ppm P base seca. Palabras clave: bacteria endofítica; solubilización de fosfatos; rábano (Raphanus sativus, L.; caña de azúcar (Saccharum híbrido.Phosphorus is an essential mineral macronutrient for plant growth and development. Although it can be found in soils in different mineral forms, its low solubility decrease its availability for plants; the nutrient should be added to crops as fertiliser. World sources of P are limited and in the near future they will suffer a remarkable decrease. Using P solubiliser microorganisms as

  13. Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane yield Produção de biomassa e presença de fungos micorrízicos arbusculares em culturas utilizadas em rotação com a cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Edmilson José Ambrosano

    2010-12-01

    Full Text Available A cana-de-açúcar (Saccharum spp. vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes e girassol (Helianthus annuus L., a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L. cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum Piper and Tracy foram as culturas que apresentaram maior percentagem de colonização por fungos micorrízicos. O girassol foi a planta de cobertura que mais extraiu nutrientes do solo, seguido por amendoim (Arachis hipogaea L. cv. IAC-Tatu e feijão-mungo (Vigna radiata L. Wilczek. A colonização por fungos micorrízicos mostrou correlação positiva com a altura de plantas de cana no primeiro corte (p = 0,01 e R = 0,52, mas não se correlacionou com a produtividade de colmos ou açúcar. No primeiro corte, o girassol foi a cultura de rotação que ocasionou o maior aumento de produtividade, da ordem de 46% em colmos e de 50% na quantidade de açúcar, em comparação com a testemunha. Com exceção dos amendoins, todas as culturas em rotação aumentaram a renda líquida do sistema na média de três cortes de cana-de-açúcar.Sugarcane (Saccharum spp. is an important crop for sugar production and agro-energy purposes in Brazil. In the sugarcane production system after a 4- to 8-year cycle crop rotation may be used before replanting sugarcane to improve soil conditions and give an extra income. This study had the objective of characterizing the biomass and the

  14. Desenvolvimento vegetativo e produção de variedades de cana-de-açúcar em relação à disponibilidade hídrica e unidades térmicas Vegetative development and production of sugarcane varieties as a function of water availability and thermic units

    Directory of Open Access Journals (Sweden)

    Alexsandro Claudio dos Santos Almeida

    2008-10-01

    Full Text Available Objetivou-se, neste trabalho, avaliar padrões de crescimento e de produção de variedades de cana-de-açúcar (Saccharum officinarum L. com os graus-dias e disponibilidade hídrica, durante os cultivos de cana-planta e cana-soca, na região dos Tabuleiros Costeiros Alagoano. O trabalho foi desenvolvido na área experimental de Agrometeorologia da Universidade Federal de Alagoas, Rio Largo-AL, num solo classificado como Latossolo Amarelo coeso argissólico. Utilizou-se o delineamento experimental de blocos cazualizados com cinco repetições e quatro tratamentos: variedades RB92579, RB931530, RB93509 e SP79-1011. As variáveis da cultura utilizadas foram: perfilhamento, altura da planta, índice de área foliar (IAF, acúmulo de matéria seca no colmo e produção final. Foram encontradas diferenças significativas entre as médias de perfilhamento das variedades em todas as épocas amostradas, de forma geral o maior perfilhamento foi observado na variedade RB92579. O crescimento das plantas apresentou curvas de formato sigmoidal, com três fases distintas. Na fase de estabelecimento da cultura, apesar de ter ocorrido ótima disponibilidade hídrica, o crescimento foi lento. Na fase de crescimento vegetativo as plantas não cresceram em potencial, porque houve deficiências hídricas. A fase final ocorreu entre 1500 e 2015 GD na cana-planta e 950 e 1800 GD na cana-soca. A variedade RB92579 alcançou valores de IAF superiores a 3, logo aos 600 GD nos dois ciclos, permanecendo o restante dos ciclos acima desse valor, contribuindo para uma melhor eficiência no uso da água e para o maior acúmulo de matéria seca.This work had the aim of evaluating the growth standard and yield of different sugarcane (Saccharum officinarum L. varieties with meteorogical parameter, during the plant and ratoon crop cane in Mesas regions of Alagoas. This work was carried out in an experimental area of Agrometeorology used a randomized outline with five repetition

  15. Use of Simple Sequence Repeat (SSR) markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp.) cultivars resistant and susceptible to red rot

    Science.gov (United States)

    In recent years SSR markers have been used widely for the genetic analysis. The objective of present research was to use SSR markers to develop DNA-based genetic identification and analyze genetic relationship of sugarcane cultivars grown in Pakistan either resistant or susceptible to red rot. Twent...

  16. Carbon fractions and soil fertility affected by tillage and sugarcane residue management an Xanthic Udult

    Directory of Open Access Journals (Sweden)

    Iara Maria Lopes

    2017-10-01

    Full Text Available The gradual change in management practices in sugarcane (Saccharum spp. production from burning straw to a green harvesting system, as well as the use of minimum soil tillage during field renovation, may affect soil fertility and soil organic matter (SOM contents. The objectives of this work were to investigate the influence of sugar cane production systems on: (1 soil fertility parameters; (2 on physical carbon fractions; (3 and on humic substance fractions, in a long-term experiment, comparing two soil tillage and two residue management systems an Xanthic Udult, in the coastal tableland region of Espírito Santo State, Brazil. The treatments consisted of plots (conventional tillage (CT or minimum tillage (MT and subplots (residue burned or unburned at harvesting, with five replicates The highest values of Ca2+ + Mg2+ and total organic carbon (TOC were observed in the MT system in all soil layers, while high values of K+ were observed in the 0.1-0.2 m layer. The CT associated with the burned residue management negatively influenced the TOC values, especially in the 0.1-0.2 and 0.2-0.4 m layers. The carbon in the humin fraction and organic matter associated with minerals were significantly different among the tillage systems; the MT showed higher values than the CT. However, there were no significant differences between the sugarcane residue management treatments. Overall, fractioning the SOM allowed for a better understanding of tillage and residue management systems effects on the soil properties.

  17. Selected beetle assemblages captured in pitfall traps baited with deer dung or meat in balsam fir and sugar maple forests of central Quebec.

    Science.gov (United States)

    Brousseau, Pierre-Marc; Cloutier, Conrad; Hébert, Christian

    2010-08-01

    Vertebrate dung and carrion are rich and strongly attractive resources for numerous beetles that are often closely linked to them. The presence and abundance of beetles exploiting such resources are influenced by various ecological factors including climate and forest cover vegetation. We studied selected assemblages of coprophilous and necrophagous beetles in Quebec along a 115-km north-south transect in three balsam fir (Abies balsamea (L.) Miller) forest sites and in a fourth forest site dominated by sugar maple (Acer saccharum Marshall), close to the southern fir site. Beetle abundance was estimated using a sampling design comprising replicated pitfall traps baited with red deer meat or dung in each site. A total of 8,511 beetles were caught and identified to family level, 95.7% of which belonged to families with known coprophilous or necrophagous behavior. Meat-baited pitfall traps caught nearly 15 times as many beetles as dung-baited traps. All Histeridae, Hydrophilidae, Scarabaeidae, and Silphidae were identified to species to examine specific diversity variation among sites. For the beetles caught in the meat-baited traps (majority of captures), decreases in abundance and species richness were observed from south to north along the fir forest transect, with evidence of decreasing specific diversity as measured by the Shannon index of diversity. Strong differences in species assemblages were also observed between the southern maple and fir forest sites. The Silphidae and Histeridae were more abundant in the maple forest, whereas the Hydrophilidae and Ptilidae were more abundant in the fir forest.

  18. Soil change induced by the application of biodigested vinasse concentrate, and its effects on growth of sugarcane

    Directory of Open Access Journals (Sweden)

    Juliana Silva

    2015-06-01

    Full Text Available Vinasse (or stillage is a byproduct from ethanol production, which contains organic matter, K, N, and other plant nutrients that is regularly used as soil fertilizer. However, high transportation costs limits its application in areas far from distilleries. The possibility of biogas production from vinasse, and the direct or indirect advantages of its use, is a way to reduce costs due to its concentration. Biodigested vinasse concentrate (BVC is an alkaline product that is very different from common vinasse. Therefore, the objective of this study was to compare the effect of BVC with common vinasse (CV or KCl, with or without N fertilization, on soil fertility and growth and nutrition of sugarcane (Saccharum officinarum L. plants. Plants were grown in pots containing Oxisol under different treatments and maintained for 60 d under greenhouse conditions; variables related to soil fertility, plant growth, and mineral nutrition were evaluated. It was observed that adding BVC induces higher soil pH (5.9 to 6.3 and lower potential acidity (13 to 10 mmol c dm-3 compared with KCl, and similar soil chemical changes to CV addition. Plants fertilized with BVC and N showed lower root dry matter (DM (4.02 g compared with those fertilized with KCl and CV (6.3 and 5.44 g, respectively. Plants fertilized with BVC have similar total DM (18.25 and 20.31 g accumulation and nutritional conditions compared with those fertilized with CV and KCl. Plants fertilized with BVC had the highest Na accumulation (0.36 and 0.48 g plant-1.

  19. Looking for age-related growth decline in natural forests: unexpected biomass patterns from tree rings and simulated mortality

    Science.gov (United States)

    Foster, Jane R.; D'Amato, Anthony W.; Bradford, John B.

    2014-01-01

    Forest biomass growth is almost universally assumed to peak early in stand development, near canopy closure, after which it will plateau or decline. The chronosequence and plot remeasurement approaches used to establish the decline pattern suffer from limitations and coarse temporal detail. We combined annual tree ring measurements and mortality models to address two questions: first, how do assumptions about tree growth and mortality influence reconstructions of biomass growth? Second, under what circumstances does biomass production follow the model that peaks early, then declines? We integrated three stochastic mortality models with a census tree-ring data set from eight temperate forest types to reconstruct stand-level biomass increments (in Minnesota, USA). We compared growth patterns among mortality models, forest types and stands. Timing of peak biomass growth varied significantly among mortality models, peaking 20–30 years earlier when mortality was random with respect to tree growth and size, than when mortality favored slow-growing individuals. Random or u-shaped mortality (highest in small or large trees) produced peak growth 25–30 % higher than the surviving tree sample alone. Growth trends for even-aged, monospecific Pinus banksiana or Acer saccharum forests were similar to the early peak and decline expectation. However, we observed continually increasing biomass growth in older, low-productivity forests of Quercus rubra, Fraxinus nigra, and Thuja occidentalis. Tree-ring reconstructions estimated annual changes in live biomass growth and identified more diverse development patterns than previous methods. These detailed, long-term patterns of biomass development are crucial for detecting recent growth responses to global change and modeling future forest dynamics.

  20. Factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass

    Science.gov (United States)

    2014-01-01

    Background Polyhydroxyalkanoates are linear biodegradable polyesters produced by bacteria as a carbon store and used to produce a range of bioplastics. Widespread polyhydroxyalkanoate production in C4 crops would decrease petroleum dependency by producing a renewable supply of biodegradable plastics along with residual biomass that could be converted into biofuels or energy. Increasing yields to commercial levels in biomass crops however remains a challenge. Previously, lower accumulation levels of the short side chain polyhydroxyalkanoate, polyhydroxybutyrate (PHB), were observed in the chloroplasts of mesophyll (M) cells compared to bundle sheath (BS) cells in transgenic maize (Zea mays), sugarcane (Saccharum sp.), and switchgrass (Panicum virgatum L.) leading to a significant decrease in the theoretical yield potential. Here we explore various factors which might affect polymer accumulation in mesophyll cells, including targeting of the PHB pathway enzymes to the mesophyll plastid and their access to substrate. Results The small subunit of Rubisco from pea effectively targeted the PHB biosynthesis enzymes to both M and BS chloroplasts of sugarcane and switchgrass. PHB enzyme activity was retained following targeting to M plastids and was equivalent to that found in the BS plastids. Leaf total fatty acid content was not affected by PHB production. However, when fatty acid synthesis was chemically inhibited, polymer accumulated in M cells. Conclusions In this study, we provide evidence that access to substrate and neither poor targeting nor insufficient activity of the PHB biosynthetic enzymes may be the limiting factor for polymer production in mesophyll chloroplasts of C4 plants. PMID:25209261

  1. Production of sugarcane and tropical grasses as a renewable energy source. Third quarterly report, December 1, 1980-February 28, 1981

    Energy Technology Data Exchange (ETDEWEB)

    Alexander, A.G.

    1981-01-01

    Research continued on tropical grasses from Saccharum and related genera as sources of intensively-propagated fiber and fermentable solids. Greenhouse-level screening for short- and intermediate-rotation grasses included further trials with legume species as potential nitrogen sources for the tropical grasses. Yields from four indigenous legumes were appreciably higher when planted in spring (May) than when planted in autumn (November). Initial data were recorded from second generation energy cane studies established during 1980. Controlled variables include varieties, harvest interval, and nitrogen input. Yield data at 6 months indicate high but essentially equal growth rates among all varieties and N-variables. This is attributed to the use of a land rotavator during seedbed preparation - the first such application of this implement on Lajas Valley soils. Total green weights were in the order of 50 to 60 tons/acre, and millable stem weights ranged from 33 to 37 tons/acre, at the 6-months harvest. Dry matter yields ranged from 8 to 11 tons/acre. Juice quality values indicated a minimal sugar content at this stage of maturity. Fiber values ranged from 7 to 14%. Field-scale studies were continued at the Hatillo demonstration site on the humid north coast and in the semi-arid Lajas Valley. For the 6-months harvest at Hatillo, total green weights for all treatments averaged 38.4 tons/acre. Variety US 67-22-2 produced 50.2 tons/acre. Millable cane yields averaged 25.3 tons/acre for all treatments and 34.7 tons/acre for variety US 67-22.2.

  2. Assessment of variables controlling nitrate dynamics in groundwater: is it a threat to surface aquatic ecosystems?

    Science.gov (United States)

    Rasiah, V; Armour, J D; Cogle, A L

    2005-01-01

    The impact of fertilised cropping on nitrate-N dynamics in groundwater (GW) was assessed in a catchment from piezometers installed: (i) to different depths, (ii) in different soil types, (iii) on different positions on landscape, and (iv) compared with the Australian and New Zealand Environmental and Conservation Council guideline values provided for different aquatic ecosystems. The GW and NO(3)-N concentration dynamics were monitored in 39 piezometer wells, installed to 5-90 m depth, under fertilized sugarcane (Saccharum officinarum-S) in the Johnstone River Catchment, Australia, from 1999 January through September 2002. The median nitrate-N concentration ranged from 14 to 1511 microg L(-1), and the 80th percentile from 0 to 1341 microg L(-1). In 34 out of the 39 piezometer wells the 80th percentile or 80% of the nitrate-N values were higher than 30 microg L(-1), which is the maximum trigger value provided in the ANZECC table for sustainable health of different aquatic ecosystems. Nitrate-N concentration decreased with increasing well depth, increasing depth of water in wells, and with decreasing relief on landscape. Nitrate-N was higher in alluvial soil profiles than on those formed in-situ. Nitrate-N increased with increasing rainfall at the beginning of the rainy season, fluctuated during the peak rainy period, and then decreased when the rain ceased. The rapid decrease in GW after the rains ceased suggested potential existed for nitrate-N to be discharged as lateral-flow into streams. This may contribute towards the deterioration in the health of down-stream aquatic ecosystems.

  3. Soil fungal and bacterial biomass determined by epifluorescence microscopy and mycorrhizal spore density in different sugarcane managements

    Directory of Open Access Journals (Sweden)

    Adriana Pereira Aleixo

    2014-04-01

    Full Text Available Crop productivity and sustainability have often been related to soil organic matter and soil microbial biomass, especially because of their role in soil nutrient cycling. This study aimed at measuring fungal and bacterial biomass by epifluorescence microscopy and arbuscular mycorrhizal fungal (AMF spore density in sugarcane (Saccharum officinarum L. fields under different managements. We collected soil samples of sugarcane fields managed with or without burning, with or without mechanized harvest, with or without application of vinasse and from nearby riparian native forest. The soil samples were collected at 10cm depth and storage at 4°C until analysis. Fungal biomass varied from 25 to 37µg C g-1 dry soil and bacterial from 178 to 263µg C g-1 dry soil. The average fungal/bacterial ratio of fields was 0.14. The AMF spore density varied from 9 to 13 spores g-1 dry soil. The different sugarcane managements did not affect AMF spore density. In general, there were no significant changes of microbial biomass with crop management and riparian forest. However, the sum of fungal and bacterial biomass measured by epifluorescence microscopy (i.e. 208-301µg C g-1 dry soil was very close to values of total soil microbial biomass observed in other studies with traditional techniques (e.g. fumigation-extraction. Therefore, determination of fungal/bacterial ratios by epifluorescence microscopy, associated with other parameters, appears to be a promising methodology to understand microbial functionality and nutrient cycling under different soil and crop managements.

  4. Quality evaluation of compost produced from agro-industrialbyproducts of sugar cane

    Directory of Open Access Journals (Sweden)

    Alexander Bohórquez

    2014-01-01

    Full Text Available Fresh by products of the sugar industry (sugarcane sludge, bagasse and vinasse incorporated into the soil generate a negative impact on plants. Therefore, compost is an alternative solution to the use of sugarcane byproducts, which must meet the requirements of the Colombian technical standard 5167 for use as biofertilizer. This study aimed to evaluate the quality of compost made from different combinations of products of the milling process of sugar cane (Saccharum officinarum L.. Composting piles were established in the Ingenio Riopaila-Castilla, Valle del Cauca, Colombia, using a complete randomized block design with five treatments and four replications. 100% sugarcane sludge (T1, 75% sugarcane sludge and 25% bagasse (T2, 50% bagasse and 50% sugarcane sludge (T3, 25% sugarcane sludge and 75% bagasse (T4 and 100% bagasse (T5, all supplemented with 2 m3 of vinasse. The response variables: pH, electrical conductivity, moisture, ash, organic matter, moisture retention, the carbon-nitrogen ratio, the total oxidizable organic carbon, total nitrogen, phosphorus, calcium, magnesium, potassium, iron, copper, manganese and zinc, were evaluated at the time when the initial compost piles were prepared, and the 42, 51, 59, 73 and 90 days after beginning the process. The results showed that the carbon-nitrogen mixtures initial ratio is critical for obtaining a good quality of compost. The T3 provided the best quality with the highest content of nutrients. The composting time ensuring adequate maturation levels for nutrients in the compost was 90 days.

  5. Photosynthetic responses to understory shade and elevated carbon dioxide concentration in 4 northern hardwood tree species

    International Nuclear Information System (INIS)

    Sefcik, L.T.; Zak, D.R.; Ellsworth, D.S.

    2006-01-01

    Stimulation of photosynthesis in response to elevated carbon dioxide (CO 2 ) varies among tree species and species groups. In this study, seedling responses to elevated atmospheric carbon dioxide (CO 2 ) concentrations and solar irradiance over 2 growing seasons were investigated for shade tolerant Acer saccharum Marsh.; Fagus grandifolia J.F. Ehrh; and shade-intolerant Prunus serotina. Seedlings were exposed to a combination of elevated and ambient concentrations of CO 2 and understory shade in open-top chambers placed in a forest understory. It was observed that the elevated CO 2 treatment increased mean light-saturated net photosynthetic rates by 63 per cent in the shade-tolerant species and 67 per cent in the shade-intolerant species. When measured at the elevated CO 2 , long-term enhancement of photosynthesis was 10 per cent lower than the instantaneous enhancement observed in ambient-CO 2 -grown plants. As the growth irradiance increased, proportional enhancement due to elevated CO 2 decreased from 97 per cent for plants grown in deep shade to 47 per cent for plants grown in moderate shade. Results indicated that in nitrogen (N) limited northern temperate forests, trees grown in deep shade may display greater photosynthetic gains from a CO 2 enriched atmosphere than trees growing in more moderate shade, due to greater down-regulation. It was concluded that if elevated CO 2 levels promote the survival of shade-intolerant species in dim understory light, the future composition and dynamics of successional forest communities may be altered. 70 refs., 2 tabs., 3 figs

  6. Rooting and dating maples (Acer) with an uncorrelated-rates molecular clock: implications for north American/Asian disjunctions.

    Science.gov (United States)

    Renner, Susanne S; Grimm, Guido W; Schneeweiss, Gerald M; Stuessy, Tod F; Ricklefs, Robert E

    2008-10-01

    Simulations suggest that molecular clock analyses can correctly identify the root of a tree even when the clock assumption is severely violated. Clock-based rooting of phylogenies may be particularly useful when outgroup rooting is problematic. Here, we explore relaxed-clock rooting in the Acer/Dipteronia clade of Sapindaceae, which comprises genera of highly uneven species richness and problematic mutual monophyly. Using an approach that does not presuppose rate autocorrelation between ancestral and descendant branches and hence does not require a rooted a priori topology, we analyzed data from up to seven chloroplast loci for some 50 ingroup species. For comparison, we used midpoint and outgroup rooting and dating methods that rely on rooted input trees, namely penalized likelihood, a Bayesian autocorrelated-rates model, and a strict clock. The chloroplast sequences used here reject a single global substitution rate, and the assumption of autocorrelated rates was also rejected. The root was placed between Acer and Dipteronia by all three rooting methods, albeit with low statistical support. Analyses of Acer diversification with a lineage-through-time plot and different survival models, although sensitive to missing data, suggest a gradual decrease in the average diversification rate. The nine North American species of Acer diverged from their nearest relatives at widely different times: eastern American Acer diverged in the Oligocene and Late Miocene; western American species in the Late Eocene and Mid Miocene; and the Acer core clade, including A. saccharum, dates to the Miocene. Recent diversification in North America is strikingly rare compared to diversification in eastern Asia.

  7. Red rot resistant transgenic sugarcane developed through expression of β-1,3-glucanase gene.

    Directory of Open Access Journals (Sweden)

    Shivani Nayyar

    Full Text Available Sugarcane (Saccharum spp. is a commercially important crop, vulnerable to fungal disease red rot caused by Colletotrichum falcatum Went. The pathogen attacks sucrose accumulating parenchyma cells of cane stalk leading to severe losses in cane yield and sugar recovery. We report development of red rot resistant transgenic sugarcane through expression of β-1,3-glucanase gene from Trichoderma spp. The transgene integration and its expression were confirmed by quantitative reverse transcription-PCR in first clonal generation raised from T0 plants revealing up to 4.4-fold higher expression, in comparison to non-transgenic sugarcane. Bioassay of transgenic plants with two virulent C. falcatum pathotypes, Cf 08 and Cf 09 causing red rot disease demonstrated that some plants were resistant to Cf 08 and moderately resistant to Cf 09. The electron micrographs of sucrose storing stalk parenchyma cells from these plants displayed characteristic sucrose-filled cells inhibiting Cf 08 hyphae and lysis of Cf 09 hyphae; in contrast, the cells of susceptible plants were sucrose depleted and prone to both the pathotypes. The transgene expression was up-regulated (up to 2.0-fold in leaves and 5.0-fold in roots after infection, as compared to before infection in resistant plants. The transgene was successfully transmitted to second clonal generation raised from resistant transgenic plants. β-1,3-glucanase protein structural model revealed that active sites Glutamate 628 and Aspartate 569 of the catalytic domain acted as proton donor and nucleophile having role in cleaving β-1,3-glycosidic bonds and pathogen hyphal lysis.

  8. Becoming less tolerant with age: sugar maple, shade, and ontogeny.

    Science.gov (United States)

    Sendall, Kerrie M; Lusk, Christopher H; Reich, Peter B

    2015-12-01

    Although shade tolerance is often assumed to be a fixed trait, recent work suggests ontogenetic changes in the light requirements of tree species. We determined the influence of gas exchange, biomass distribution, and self-shading on ontogenetic variation in the instantaneous aboveground carbon balance of Acer saccharum. We quantified the aboveground biomass distributions of 18 juveniles varying in height and growing in low light in a temperate forest understory in Minnesota, USA. Gas exchange rates of leaf and stem tissues were measured, and the crown architecture of each individual was quantified. The YPLANT program was used to estimate the self-shaded fraction of each crown and to model net leaf-level carbon gain. Leaf respiration and photosynthesis per gram of leaf tissue increased with plant size. In contrast, stem respiration rates per gram of stem tissue declined, reflecting a shift in the distribution of stem diameter sizes from smaller (with higher respiration) to larger diameter classes. However, these trends were outweighed by ontogenetic increases in self-shading (which reduces the net photosynthesis realized) and stem mass fraction (which increases the proportion of purely respiratory tissue) in terms of influence on net carbon exchange. As a result, net carbon gain per gram of aboveground plant tissue declined with increasing plant size, and the instantaneous aboveground light compensation point increased. When estimates of root respiration were included to model whole-plant carbon gain and light compensation points, relationships with plant size were even more pronounced. Our findings show how an interplay of gas exchange, self-shading, and biomass distribution shapes ontogenetic changes in shade tolerance.

  9. PENGARUH PEMBERIAN SARI TEBU TERHADAP KADAR KOLESTEROL DARAH PADA MENCIT

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    Rahma Widyastuti

    2017-12-01

    abstract Sugarcane (Saccharum officinarum L. is a plant known by the public as a refreshing drink that tastes sweet and the price is quite economical and readily available. Sugarcane contains octacosanol compounds that can lower cholesterol levels in the blood. Excessive cholesterol (hypercholesterolemia a threat that is feared because as one of the causes of coronary heart disease (CHD and stroke. The purpose of this study was to analyze the levels of cholesterol in mice, to analyze cholesterol levels in mice after the administration of sugar cane juice, to analyze the effect of sugar cane juice on cholesterol levels in mice. This type of research conducted by a group of experimental methods with populations of mice (Mus musculus were obtained from farms Bojonegoro. The research sample selected by criteria mice aged 2-3 months, weighing between 20-40 grams of the male sex, the amount of sample used two groups of mice, each group consisted of 16 mice. From the results of blood cholesterol levels in mice after the administration of sugar cane juice, it is known that the average cholesterol level in the control group was 148.8750 mg / dl and value - average blood cholesterol treatment group was 118.2500 mg / dl. Statistically analyzed using a paired t test t value -3.799 obtained with significant value. (p = 0.001 which is less than 0.05 then there is the effect (Ho is rejected (t = -2.040 table. So we can conclude there is the effect of sugar cane juice on blood cholesterol levels in mice. Key word : Sugarcane, blood cholesterol levels

  10. Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera, and comparative analyses with other grass genomes

    Science.gov (United States)

    Saski, Christopher; Lee, Seung-Bum; Fjellheim, Siri; Guda, Chittibabu; Jansen, Robert K.; Luo, Hong; Tomkins, Jeffrey; Rognli, Odd Arne; Clarke, Jihong Liu

    2009-01-01

    Comparisons of complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera to six published grass chloroplast genomes reveal that gene content and order are similar but two microstructural changes have occurred. First, the expansion of the IR at the SSC/IRa boundary that duplicates a portion of the 5′ end of ndhH is restricted to the three genera of the subfamily Pooideae (Agrostis, Hordeum and Triticum). Second, a 6 bp deletion in ndhK is shared by Agrostis, Hordeum, Oryza and Triticum, and this event supports the sister relationship between the subfamilies Erhartoideae and Pooideae. Repeat analysis identified 19–37 direct and inverted repeats 30 bp or longer with a sequence identity of at least 90%. Seventeen of the 26 shared repeats are found in all the grass chloroplast genomes examined and are located in the same genes or intergenic spacer (IGS) regions. Examination of simple sequence repeats (SSRs) identified 16–21 potential polymorphic SSRs. Five IGS regions have 100% sequence identity among Zea mays, Saccharum officinarum and Sorghum bicolor, whereas no spacer regions were identical among Oryza sativa, Triticum aestivum, H. vulgare and A. stolonifera despite their close phylogenetic relationship. Alignment of EST sequences and DNA coding sequences identified six C–U conversions in both Sorghum bicolor and H. vulgare but only one in A. stolonifera. Phylogenetic trees based on DNA sequences of 61 protein-coding genes of 38 taxa using both maximum parsimony and likelihood methods provide moderate support for a sister relationship between the subfamilies Erhartoideae and Pooideae. PMID:17534593

  11. Mites associated with sugarcane crop and with native trees from adjacent Atlantic forest fragment in Brazil.

    Science.gov (United States)

    Duarte, Mércia E; Navia, Denise; dos Santos, Lucas R; Rideiqui, Pedro J S; Silva, Edmilson S

    2015-08-01

    In some Brazilian regions the Atlantic forest biome is currently restrict to fragments occurring amid monocultures, as sugarcane crops in the Northeast region. Important influence of forest remnants over mite fauna of permanent crops have been showed, however it has been poorly explored on annual crops. The first step for understanding ecological relationship in an agricultural systems is known its composition. The objective of this study was to investigate the plant-inhabiting mite fauna associated with sugarcane crop (Saccharum officinarum L.) (Poaceae) and caboatã (Cupania oblongifolia Mart.) (Sapindaceae) trees in the state of Alagoas, Brazil. Sugarcane stalks and sugarcane and caboatã apical, middle and basal leaves were sampled. A total of 2565 mites were collected from sugarcane and classified into seven families of Trombidiformes and Mesostigmata orders, with most individuals belonging to the Eriophyidae, Tetranychidae and Tarsonemidae families. Among predatory mites, the Phytoseiidae were the most common. A total of 1878 mites were found on C. oblongifolia and classified into 13 families of Trombidiformes and Mesostigmata orders. The most abundant phytophagous mite family on caboatã was also Eriophyidae. In contrast to sugarcane, Ascidae was the most common predatory mite family observed in caboatã. No phytophagous species were common to both sugarcane and C. oblongifolia. However two predatory mites were shared between host plants. Although mites associated with only one native species in the forest fragment were evaluated in this study, our preliminary results suggest Atlantic forest native vegetation can present an important role in the sugarcane agricultural system as a source of natural enemies.

  12. Efeitos de herbicidas nos teores de macronutrientes e nas características tecnológicas da cana-de-açúcar (Saccharum spp.. I - Misturas de herbicidas em pós-emergência Effect of herbicides on the macronutrient levels and on the tecnologic characteristics of sugarcane (Saccharum spp. I - Mixtures of postemergence herbicides

    Directory of Open Access Journals (Sweden)

    R. Victoria Filho

    1980-12-01

    Full Text Available A presente pesquisa foi conduzida em um Latossol Roxo, com 3,9% de matéria orgânica, na Usina São Carlos, município de Jaboticabal - SP com o objetivo de verificar o comportamento das principais misturas de herbicidas aplicadas em pós-emergência e suas possíveis interferências nos teores de macronutrientes, e no desenvolvimento da variedade CB 41-14. Os tratamentos utilizados com as respectivas doses do ingrediente ativo em kg/ha foram: ametryne + 2,4 - D (formulação comercial a 1,47 - 2,03, diuron + 2,4 -D a 1,60 + 2,16; ametryne + 2,4-D a 1,60 + 2,16; alachlor + 2,4-D a 1,09 + 1,80; asulam + ioxynil - 2,4-D a 2,80 + 1,75; MCPA + 2,4-D a 0,83 + 0,83; oxadiazon + 2,4 -D a 0,50 + 2,16; ametryne + simazine + 2,4-D a 1,25 + 1,25 + 2,16; ametryne + secbumetone + 2,4-D a 2,00 + 2,00 + 2,16; diuron + hexazinone + surfatante a 0,80 + 0,45 + 0,5% e MCPA + 2,3,6-TBA + pendimethalin + surfatante a 1,50 + 0,48 + 0,66 + 0,5%. O controle das plantas daninhas foi avaliado através de contagens por espécie botânica e por avaliações visuais . Sobre a cultura os efeitos foram constatados pela contagem da brotação inicial, avaliações visuais dos efeitos fitotóxicos, análise dos teores de macronutrientes aos 5 e 8 meses, medidas do comprimento dos colmos, análise dos teores de fibra, pol, brix, % de cana e pureza, assim como valores de pol/ha, e peso de colmos por hectare. Não houve interferência das diferentes misturas de herbicidas na brotação inicial, no comprimento médio dos colmos, e nos teores de fibra, pol, brix e pureza por ocasião da colheita. Em relação às principais plantas daninhas presentes na área, que eram capim-colchão (Digitaria sanguinalis (L. Scop. capim-coloniâo (Panicum maximum Jacq, beldroega (Portulacca oleracea L. e caruru (Amaranthus spp, as melhores porcentagens de controle foram obtidas com as misturas de ametryne + 2,4-D, MCPA + 2,4-D, oxadiazon + 2,4-D, ametryne + secbumetone, diuron + hexazinone e MCPA + 2,3,6-TBA + pendimethalin. Aos cinco meses o teor de P foi diminuido pela mistura de ametryne + secbumetone + 2,4-D, e o teor de Ca decresceu pelas misturas de diuron + 2,4-D, ametryne + 2,4-D, alachlor + 2,4-D, asulam + ioxynil + 2,4-D, MCPA + 2,4-D, oxadiazon + 2,4-D, ametryne + simazine + 2,4-D e ametryne + secbumetone + 2,4-D. Já aos oito meses não houve diferença estatística para qualquer dos nutrientes estudados. Os teores de pol/ha e produção de colmos/ha não foram significativamente afetados por qualquer dos tratamentos quando comparados com a testemunha com capina. Todavia, todos os tratamentos apresentaram produção inferior a esta, sugerindo certa precaução com as aplicações pós-emergentes em canade-açúcar.The present research was conducted in the São Carlos Sugar Mill. Jaboticabal - SP in a oxisol (Latossolic B containing 3,9% organic matter. with the obje tive to study the behavior of the main post-emergence herbicide mixtures, in the sugarcane crop for weed control, and their interferences on the macronutrient levels and development of CB 41-14 variety. The treatments in kg of active ingredient per hectare were: ametryne + 2,4-D (comercial formulation at 1,47 -t 2,03; diuron + 2,4-D at 1,60 + 2,16: ametryne + 2,4-D at 1,60 + 2,16; alachlor + 2,4-D at 1,09 + 1,08; asulam + ioxynil 2,4 -D at 2,8 + 1,75; MCPA + 2,4-D at 0,83 + 0,83; oxadiazon 2,4-D at 0,50 + 2,16; ametryne + simazine + 2,4-D at 1,25 1,25 + 2,16; ametryn + secbumeton + 2,4 -D at 2,00 + 2,00 + 2,16; diuron + hexazinone + surfactant at 0,80 + 0,45 + 0,5% and MCPA + 2,3 ,6 -TBA + pendimethalin + surfactant at 1,50 + 0,48 + 0,66 + 0,5% . The weed control was evaluated by counting the surviving weeds, and by visual evaluation according to the scale of the "AssociacionLatinoamericana de Malezas" (ALAM. The effects on the crop were evaluated by counting the fresh-emerging sugarcane plants, visual evaluating of herbicide injuries, analysis of macronutrients levels in the leaves at 5 and 8 months after planting, measures of the lenght of the culms, analysis of fibers, brix, purity and poi and the values of pot/ha and weight of culms/ha. Any interference of the herbicide mixtures. was not observed on the ini tial growth, the average lenght of the culms, the fiber, pol, brix or purity. The best weed control was obtained by the mixtures ametryn + 2,4-D, MCPA + 2,4-D, oxadiazon + 2,4-D, ametryn + secbumeton, diuron + hexazinone and MCPA + 2,3,6 TBA + pendimethalin, and the principal weeds present in the area were Digitaria sanguinalis (L . Scop, Panicum maximum Jacq., Portulacca oleracea L. and Amaranthus spp. The P content level was reduced by ametryn + secbumeton t 2,4-D and the Ca reduced by diuron + 2,4-D, ametryn ; 2,4-D, alachlor + 2,4-D, asulam + ioxynil + 2,4-D, MCPA + 2,4-D, oxadiazon + 2,4-D, ametryn + simazine + 2,4-D and ametryn + secbumeton + 2,4-D at 5 months. At 8 months no effect on the macronutrient levels was observed. The values of po l/ha and weight of culms/ha were not affected by the treatments when compared with the hoed control, but all treatments yielded less than the hoed control.

  13. Digestibilidade in situ de cana de açúcar (Saccharum officinarum nas formas natural ou ensilada, adicionadas ou não de uréia

    Directory of Open Access Journals (Sweden)

    Carlos de Sousa Lucci

    2006-08-01

    Full Text Available Doze ovinos com cânulas de rúmen foram empregados para comparar seis tratamentos, dispostos em um arranjo fatorial 2 x 3: cana de açúcar nas formas fresca (CAF ou ensilada (CAS x teores de uréia iguais a 0,0%, 0,5% e 1,0%. Foram estimadas: taxas de degradabilidade efetiva (DGE dos alimentos volumosos, concentrações de ácidos graxos voláteis(AGV e de nitrogênio amoniacal (N-NH3 e valores de pH do conteúdo do rúmen, além de concentrações de N-uréico no sangue. As taxas de DGE da matéria seca (MS mostraram-se semelhantes para CAF e CAS. A adição de teores crescentes de uréia às forragens resultou em diminuição linear da DGE da MS tanto da CAF como da CAS. No caso da CAS, a DGE da fibra em detergente neutro diminuiu linearmente com concentrações crescentes de uréia. Os valores de pH do conteúdo ruminal foram maiores para CAS em relação a CAF, mas não ocorreram diferenças devidas à adição de uréia. Os teores do conteúdo ruminal em total de AGV e os de ácido propiônico foram maiores para a CAF em relação a CAS; os de ácido acético, ao contrário, foram maiores para a CAS. Os teores de N-NH3 do conteúdo ruminal foram maiores para a CAS que para a CAF. Nos tratamentos com CAF e nos com CAS, as concentrações de N-NH3 no conteúdo ruminal e de N-uréico no sangue aumentaram linearmente com maiores adições de uréia. Concluiu-se que a adição de 0,5% e 1,0% uréia às forragens frescas ou ensiladas de cana-de-açucar não mostrou resultados satisfatórios e que CAF apresentou indícios de melhor qualidade nutricional que CAS.

  14. Producción y evaluación del proceso de compostaje a partir de desechos agroindustriales de Saccharum officinarum (caña de azúcar

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    GORDILLO, F.

    2011-08-01

    Full Text Available El presente estudio tuvo como finalidad el aprovechamiento de los residuos azucareros de forma técnicamente viable por medio de la producción y evaluación del proceso de compostaje. Para lograr los objetivos se establecieron combinaciones de tres tipos de materia prima, dos fuentes de microorganismos y dos métodos de aireación. Las pilas se establecieron bajo un diseño de tres factores, y se valoraron dos veces por semana los parámetros: temperatura, pH y conductividad eléctrica. Otros parámetros como materia orgánica, población microbiana, porcentaje de humedad, macro y micronutrimentos, cromatografía de Pfeifer y relación C/N, seevaluaron al inicio y al final del proceso. El mejor método fue el de aireación por volteos, y la formulación más equilibrada para macro y micronutrimentos y mayor concentración de materia orgánica fue la formulación con 50% de bagazo, 25% de cachaza y 25% de ceniza. Al finalizar el proceso, la formulación conformada por 40% bagazo, 30% cachaza y 30% ceniza tuvo la relación C/N más cercana a los valores recomendados entre 20-30. Los microorganismos comerciales mantuvieron una mayor población microbiana durante el proceso hasta su culminación. La temperatura llegó a la temperatura inicial de 45º C; el pH para el proceso fue el óptimo de 8 a 8.5 aunque finalizó elevado 9 a 9.5.

  15. Correlation of foliage and litter chemistry of sugar maple, Acer saccharum, as affected by elevated CO2 and varying N availability, and effects on decomposition

    Science.gov (United States)

    J. S. King; K. S. Pregitzer; D. R. Zak; M. E. Kubiske; W. E. Holmes

    2001-01-01

    Rising atmospheric carbon dioxide has the potential to alter leaf litter chemistry, potentially affecting decomposition and rates of carbon and nitrogen cycling in forest ecosystems. This study was conducted to determine whether growth under elevated atmospheric CO2 altered the quality and microbial decomposition of leaf litter of a widely...

  16. Composição químico-bromatológica de variedades de cana-de-açúcar (Saccharum spp L. com diferentes ciclos de produção (precoce e intermediário em três idades de corte Chemical composition of sugar cane varieties (Saccharum spp L. with different cycles of production in three cut time

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    Alberto Magno Fernandes

    2003-08-01

    Full Text Available Foram objetivos deste trabalho determinar a composição químico- bromatológica, as frações potencialmente degradável (B2 e indegradável (C da FDN dos carboidratos totais e estimar a repleção ruminal da cana-de-açúcar com diferentes ciclos de produção (precoce e intermediário, em três idades de corte (426, 487 e 549 dias. As análises laboratoriais consistiram na determinação da matéria seca (MS, matéria orgânica, matéria mineral, proteína bruta (PB, extrato etéreo, lignina, fibra em detergente neutro (FDN, FDN corrigida para cinzas e proteína, fibra em detergente ácido (FDA, proteína insolúvel em detergente neutro, proteína insolúvel em detergente ácido e proteína solúvel em detergente neutro. Os nutrientes digestíveis totais (NDT foram calculados por intermédio da composição química. As frações B2, C e a taxa de digestão da fibra, bem como a repleção ruminal, foram estimadas através de parâmetros cinéticos obtidos a partir da incubação in situ. O avanço da idade de corte propiciou aumento nos teores de MS em 9,5%. As variedades intermediárias apresentaram NDT superiores às precoces, as quais destacaram-se pelos mais elevados teores de FDN e FDA, cujos respectivos valores foram 487,56 e 471,03, e 287,87 e 247,54 g/kg MS para as variedades precoces e intermediárias, respectivamente. O NDT aumentou linearmente com a idade de corte, variando de 62,45 a 63,50%; contudo, os teores de FDN e FDA apresentaram comportamento quadrático. As variedades precoces apresentaram maior teor de PB que as intermediárias somente na idade ao corte de 549 dias, contrariamente, o teor de brix foi superior para as variedades intermediárias no último corte. As variedades precoces apresentaram maior repleção ruminal total e menor taxa de digestão da fibra. A fração B2 da fibra, foi reduzida e a C foi linearmente aumentada com a idade das plantas.The objectives of this work were to determine the chemical composition, the potentialy degradable fraction of NDF (B2 and undegradable fraction (C and to estimate the ruminal fill of sugar cane with different cycles of production (early and intermediate, in three cut times (426, 487 and 549 days. The laboratorial analysis consisted in dry matter (DM, organic matter, ash, crude protein (CP, ether extract, lignin, neutral detergent fiber (NDF, neutral detergent fiber corrected for ash and protein, acid detergent fiber (ADF, neutral detergent insoluble protein, acid detergent insoluble protein and neutral detergent protein soluble. The TDN was calculated by chemical composition. The degradable and undegradable fractions, and fiber digestion rate, as well as the ruminal fill were estimated by kinetic parameters obtained throught in situ incubation. The advanced cut time increased the DM in 9.5%. The intermediate varieties presented higher TDN than early varieties, which had the highest contents of NDF and ADF, whose respective values were 487.56 and 471.03, and 287.87 and 247,54 g/kg DM for the early and intermediate varieties, respectively. The TDN increased linearly with the cut time, varying from 62.45 to 63.50%; however the NDF and ADF contents presented quadratic behavior. The early varieties presented higher content of CP than the intermediate only in the cut time of 549 days; contrarily, the brix of the sugar cane was superior to the intermediate varieties in the last cut. The early varieties presented larger total ruminal fill and lower fiber digestion rate. The degradable fraction of the fiber was reduced and the undegradable fraction was linearly increased with the age of the plants.

  17. Efectos de la calidad de la caña semilla en los componentes del rendimiento cultural de las variedades CP65-357 y LCP85-384 (Saccharum spp. según diferentes edades de corte (Parte I Effects of seedcane quality on yielding components of varieties CP65-357 and LCP85-384 (Saccharum spp. in different crops (Part I

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    María I. Cuenya

    2007-06-01

    Full Text Available Las principales variedades de caña de azúcar cultivadas en Tucumán mostraron, hasta hace pocos años, una elevada infección con achaparramiento de la caña soca, enfermedad sistémica causada por Leifsonia xyli subsp. xyli, ampliamente reconocida por su efecto detrimental sobre el rendimiento cultural. Esta situación fue consecuencia de que los productores cañeros utilizaban caña semilla proveniente de lotes comerciales en lugar de caña semilla de semilleros saneados. El objetivo del presente trabajo fue evaluar el efecto de la calidad de la semilla en los componentes del rendimiento cultural en LCP85-384 y CP65-357, dos variedades ampliamente difundidas en Tucumán. Para cada variedad se evaluaron dos alternativas con respecto a la calidad de la semilla: Semilla de Alta Calidad (SAC proveniente de micropropagación in vitro y Semilla Comercial (SC proveniente de un cañaveral comercial. Se implantó un ensayo de acuerdo a un diseño en bloques completos al azar con seis repeticiones. En caña planta, soca 1 y soca 2 se evaluaron: número de tallos/parcela, peso, altura y diámetro del tallo y rendimiento de caña/ha. Se efectuaron análisis de la varianza y pruebas de comparación de medias (DLS de Fisher. En ambas variedades los componentes del rendimiento cultural más afectados por la calidad de la semilla utilizada fueron el peso y la altura de tallos a través de edades de corte. Para CP65-357 y LCP85-384 los tratamientos con SAC produjeron, en promedio, tallos más altos y más pesados en comparación con los tratamientos obtenidos a partir de SC. El diámetro de tallos no se afectó por la calidad de la semilla. Se detectaron importantes disminuciones en el rendimiento cultural (t caña/ha por la utilización de SC (10-25% en CP65-357 y 19-25% en LCP85-384 a través de las edades de corte. Estos resultados sustentan la utilización generalizada de semilleros saneados, sistema puesto en marcha por la EEAOC a partir del año 2000 en toda el área cañera de Tucumán.Until recently, the main sugarcane varieties planted in Tucumán showed high levels of infection by Ratoon Stunting Disease (RSD, a systemic disease caused by Leifsonia xyli subsp. xyli and widely known for its detrimental effect on crop yield. This was a consequence of the fact that sugarcane growers used commercial seedcane instead of healthy seedcane for new plantations. The objective of this work was to evaluate the effect of seedcane quality on yield components in LCP85-384 and CP65-357, two varieties widely spread in Tucumán. For each variety, two alternatives were tested: healthy seedcane (HS from a nursery obtained by means of in vitro micropropagation and seedcane from a commercial plantation (CS. A randomized block design trial with six replicates was planted. Yield components evaluated in the plant-cane and first- and second-ratoon growing seasons included: stalk number, weight, height, diameter and cane yield/ha. These variables were studied by means of ANOVA and T-test for mean comparison. In both varieties and in different crops, the yield components most affected by seedcane quality were stalk height and weight. In CP65-357 and LCP85-384, the HS treatment produced taller and heavier stalks when compared to the CS treatment. Stalk diameter was not affected by seedcane quality. Considerable cane yield decrease was detected using CS (10-25% for CP65-357 and 19-25% for LCP85-384 in different crop planting cycles. These results support healthy nursery technology, established since 2000 by EEAOC and widely used by sugarcane growers in Tucumán.

  18. Evaluación del comportamiento productivo de cultivares de caña de azúcar (Saccharum spp. a través de diferentes ambientes en Tucumán, aplicando la técnica estadística "GGE biplot" Assessment of sugarcane cultivars (Saccharum spp. productive behaviour through different environments in Tucumán by means of "GGE biplot" technique

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    Ernesto R. Chavanne

    2007-12-01

    Full Text Available En el presente trabajo, se utilizó la técnica estadística "GGE biplot" como herramienta de análisis para explorar el efecto combinado del genotipo y de la interacción genotipo-ambiente en dos grupos de cultivares de caña de azúcar evaluados en seis localidades del área cañera de Tucumán entre 2002 y 2006. Los ensayos fueron implantados de acuerdo a un diseño en bloques completos al azar con tres repeticiones. En este estudio se analizaron los datos de rendimiento de azúcar/ha determinados en el mes de mayo. El análisis "GGE biplot" permitió discriminar a los genotipos de acuerdo a su producción de azúcar/ha y a su estabilidad relativa a través de ambientes. Los genotipos más destacados fueron identificados como aquellos que mostraron mayores valores de CP1 y valores de CP2 próximos a cero. La aplicación de este método de análisis permitió además, detectar genotipos adaptados a ambientes específicos. Los análisis "GGE biplot" explicaron el 58% y 64% de los efectos combinados de genotipo y de interacción genotipo-ambiente en los grupos 1 y 2 de cultivares de caña de azúcar, respectivamente. Se destaca en general la simplicidad del gráfico de "GGE biplot" para la identificación de genotipos superiores de caña de azúcar, cuando estos son evaluados a través de diferentes ambientes."GGE biplot" technique was used in this work to analyze the effects of genotype and genotype-environment interaction on sugarcane yield per ha during May, considering two sugarcane genotype groups evaluated from 2002 to 2006 on six different sites in the sugarcane growing area in Tucumán. The trials were conducted using a randomized complete block design with three replications. Data concerning sugar yield during May were analyzed. The "GGE biplot" technique allowed identifying genotypes with higher sugar yield per ha in May and their relative stability through environments. The outstanding genotypes were identified as those that showed higher PC1 values and PC2 values close to zero. This method also allowed detecting the adaptability of genotypes to specific enviroments. The "GGE biplot" analysis accounted for 58% and 64% of the combined effects of genotype and genoype-environment interaction in groups 1 and 2, respectively. The simplicity of the "GGE biplot" display for identifying outstanding sugarcane genotypes evaluated through different environments is remarkable.

  19. Degradabilidade do capim-elefante (Pennisetum purpureum, Schum. e da cana-de-açúcar (Saccharum officinarum, L. mais uréia no rúmen de vacas mestiças Holandês × Zebu em lactação Rumen degradability of elephantgrass (Pennisetum purpureum, Schum. and sugarcane (Saccharum officinarum, L. plus urea in crossbred lactating cows

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    F.C.F. Lopes

    1999-08-01

    Full Text Available Elephantgrass shows a high stocking rate only during the rainy season. Forage production is drastically reduced in the dry season. One of the alternatives to minimize this problem is the supplementation with sugarcane plus urea. In this trial the main goal was to estimate the dry matter intake (DMI of elephantgrass, chopped sugarcane plus 1% urea and their degradability in three lactating (11.6kg/milk/day rumen fistulated crossbred cows (472kg/L.W. grazing elephantgrass during the dry season. The cows had access during three consecutive night to the paddocks grazed every 30 days in a stocking rate, of 4.5 cows/hectare. Between the two milking times, cows received chopped sugarcane plus 1% urea. The DMI was on average 1.01%/L.W. for the elephantgrass and 0.88%/L.W. for the sugarcane plus 1% urea. Total DMI, taking into account both roughage and concentrate (2kg/cow/day was 2.25%/L.W. The effective rumen degradability (ED, considering the rate of passage in the rumen of 5%/hour was 44.46% for elephantgrass and 41.94% for sugarcane plus 1% urea, DM. The elephantgrass ED crude protein was 48.58% and its neutral detergent fiber 33.82%. During the dry season lactating crossbred cows grazing elephantgrass have to receive a roughage supplement and concentrate to produce around to 12kg of milk per day.

  20. Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis

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    Carla P. Coelho

    2014-05-01

    Full Text Available Agriculturally important grasses such as rice, maize and sugarcane are evolutionarily distant from Arabidopsis, yet some components of the floral induction process are highly conserved. Flowering in sugarcane is an important factor that negatively affects cane yield and reduces sugar/ethanol production from this important perennial bioenergy crop. Comparative studies have facilitated the identification and characterization of putative orthologs of key flowering time genes in sugarcane, a complex polyploid plant whose genome has yet to be sequenced completely. Using this approach we identified phosphatidylethanolamine-binding protein (PEBP gene family members in sugarcane that are similar to the archetypical FT and TFL1 genes of Arabidopsis that play an essential role in controlling the transition from vegetative to reproductive growth. Expression analysis of ScTFL1, which falls into the TFL1-clade of floral repressors, showed transcripts in developing leaves surrounding the shoot apex but not at the apex itself. ScFT1 was detected in immature leaves and apical regions of vegetatively growing plants and, after the floral transition, expression also occurred in mature leaves. Ectopic over-expression of ScTFL1 in Arabidopsis caused delayed flowering in Arabidopsis, as might be expected for a gene related to TFL1. In addition, lines with the latest flowering phenotype exhibited aerial rosette formation. Unexpectedly, over-expression of ScFT1, which has greatest similarity to the florigen-encoding FT, also caused a delay in flowering. This preliminary analysis of divergent sugarcane FT and TFL1 gene family members from Saccharum spp. suggests that their expression patterns and roles in the floral transition has diverged from the predicted role of similar PEBP family members.

  1. Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis.

    Science.gov (United States)

    Coelho, Carla P; Minow, Mark A A; Chalfun-Júnior, Antonio; Colasanti, Joseph

    2014-01-01

    Agriculturally important grasses such as rice, maize, and sugarcane are evolutionarily distant from Arabidopsis, yet some components of the floral induction process are highly conserved. Flowering in sugarcane is an important factor that negatively affects cane yield and reduces sugar/ethanol production from this important perennial bioenergy crop. Comparative studies have facilitated the identification and characterization of putative orthologs of key flowering time genes in sugarcane, a complex polyploid plant whose genome has yet to be sequenced completely. Using this approach we identified phosphatidylethanolamine-binding protein (PEBP) gene family members in sugarcane that are similar to the archetypical FT and TFL1 genes of Arabidopsis that play an essential role in controlling the transition from vegetative to reproductive growth. Expression analysis of ScTFL1, which falls into the TFL1-clade of floral repressors, showed transcripts in developing leaves surrounding the shoot apex but not at the apex itself. ScFT1 was detected in immature leaves and apical regions of vegetatively growing plants and, after the floral transition, expression also occurred in mature leaves. Ectopic over-expression of ScTFL1 in Arabidopsis caused delayed flowering in Arabidopsis, as might be expected for a gene related to TFL1. In addition, lines with the latest flowering phenotype exhibited aerial rosette formation. Unexpectedly, over-expression of ScFT1, which has greatest similarity to the florigen-encoding FT, also caused a delay in flowering. This preliminary analysis of divergent sugarcane FT and TFL1 gene family members from Saccharum spp. suggests that their expression patterns and roles in the floral transition has diverged from the predicted role of similar PEBP family members.

  2. Anthropogenic nitrogen deposition ameliorates the decline in tree growth caused by a drier climate.

    Science.gov (United States)

    Ibáñez, Inés; Zak, Donald R; Burton, Andrew J; Pregitzer, Kurt S

    2018-02-01

    Most forest ecosystems are simultaneously affected by concurrent global change drivers. However, when assessing these effects, studies have mainly focused on the responses to single factors and have rarely evaluated the joined effects of the multiple aspects of environmental change. Here, we analyzed the combined effects of anthropogenic nitrogen (N) deposition and climatic conditions on the radial growth of Acer saccharum, a dominant tree species in eastern North American forests. We capitalized on a long-term N deposition study, replicated along a latitudinal gradient, that has been taking place for more than 20 yr. We analyzed tree radial growth as a function of anthropogenic N deposition (ambient and experimental addition) and of summer temperature and soil water conditions. Our results reveal that experimental N deposition enhances radial growth of this species, an effect that was accentuated as temperature increased and soil water became more limiting. The spatial and temporal extent of our data also allowed us to assert that the positive effects of growing under the experimental N deposition are likely due to changes in the physiological performance of this species, and not due to the positive correlation between soil N and soil water holding capacity, as has been previously speculated in other studies. Our simulations of tree growth under forecasted climate scenarios specific for this region also revealed that although anthropogenic N deposition may enhance tree growth under a large array of environmental conditions, it will not mitigate the expected effects of growing under the considerably drier conditions characteristic of our most extreme climatic scenario. © 2018 by the Ecological Society of America.

  3. Efeito da escória de siderurgia e calcário na disponibilidade de fósforo de um Latossolo Vermelho-Amarelo cultivado com cana-de-açúcar Effect of slag and limestone on the availability of phosphorus of an Oxisol planted with sugarcane

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    Renato de Mello Prado

    2001-09-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da escória de siderurgia e calcário na concentração do P disponível em um Latossolo Vermelho-Amarelo, cultivado com cana-de-açúcar (Saccharum spp., em Ituverava, SP. Foram aplicados calcário calcítico e a escória de siderurgia, objetivando elevar a saturação por bases a 50%, 75% e 100%, e usou-se testemunha (sem correção. O efeito linear da escória de siderurgia no P disponível do solo, em contraste com a ausência de relação quando se aplicou calcário, sugere que o efeito positivo da escória de siderurgia se deve mais ao efeito do silicato, de saturar os sítios de adsorção de P, do que ao efeito de aumento do pH.The aim of this work was to evaluate the effect of slag and limestone, on the available P concentration of the soil planted with sugarcane. A field experiment was conducted in Ituverava, SP, Brazil, in a Red-Yellow Oxisol. Calcitic limestone and the slag were added in order to increase the base saturation to 50%, 75% and 100%, in addition to the control without correction. The linear effect of the slag on the soil available P, contrasting the lack of relationship when the limestone was applied, suggests that the positive effect of the slag is mainly due to the silicate effect than to the pH effect.

  4. Nutrient Partitioning and Stoichiometry in Unburnt Sugarcane Ratoon at Varying Yield Levels

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    José Marcos Leite

    2016-04-01

    Full Text Available Unraveling nutrient imbalances in contemporary agriculture is a research priority to improve whenever possible yield and nutrient use efficiency in sugarcane (Saccharum spp. systems while minimizing the costs of cultivation (e.g., use of fertilizers and environmental concerns. The main goal of this study was therefore to investigate biomass and nutrient [nitrogen (N, phosphorus (P, and potassium (K] content, partitioning, stoichiometry and internal efficiencies in sugarcane ratoon at varying yield levels. Three sites were established on highly weathered tropical soils located in the Southeast region of Brazil. At all sites, seasonal biomass and nutrient uptake patterns were synthesized from four sampling times taken throughout the sugarcane ratoon season. At all sites, in-season nutrient partitioning (in diverse plant components, internal efficiencies (yield to nutrient content ratio and nutrient ratios (N:P and N:K were determined at harvesting. Sugarcane exhibited three distinct phases of plant growth, as follows: lag, exponential-linear, and stationary. Across sites, nutrient requirement per unit of yield was 1.4 kg N, 0.24 kg P, and 2.7 kg K per Mg of stalk produced, but nutrient removal varied with soil nutrient status (based on soil plus fertilizer nutrient supply and crop demand (potential yield. Dry leaves had lower nutrient content (N, P, and K and broader N:P and N:K ratios when compared with tops and stalks plant fractions. Greater sugarcane yield and narrowed N:P ratio (6:1 were verified for tops of sugarcane when increasing both N and P content. High-yielding sugarcane systems were related to higher nutrient content and more balanced N:P (6:1 and N:K (0.5:1 ratios.

  5. Effect of 15 N-urea rates on the rates on the accumulation of nitrogen by the sugarcane-plant in cane field renovation areas picked with and without burning

    International Nuclear Information System (INIS)

    Vitti, Andre Cesar.; Ocheuze Trivelin, Paulo Cesar; Wagner de Oliveira, Mauro; De Castro Gava, Glauber Jose; Sarries, Gabriel Adrian

    1999-01-01

    The main goal of this work was the evaluation of the nitrogen accumulated in the sugarcane (Saccharum spp.), the contribution and the recovery of the fertilizer by the culture, both calculated by the isotopic technique . The experiment was conducted in 220-liter vase and in a open atmosphere. A randomized block design was performed with 2x4-factorial treatment arrangements with three replications. The factors were: 1) differentiated addition of two amounts of cultural wastes to the soil, equivalent to 13.2 and 19.5 t ha -1 of dry material, simulating cane-field renovation conditions in areas picked with or without burning (CQ and SQ); 2) four N-urea rates (10.082% in 15 N-atoms), equivalent to 0; 30; 60; 90 kg ha -1 . The analysis of variance of the results showed that there were no significant differences between CQ and SQ treatments. Out of the total accumulated N in the plant, an average of 20.55% was stored in the stalks; 19% in the apexes; 27% in the root system, and 33% in dry leaves. Therefore, around 80% of the nitrogen would remain in the area after the harvest without burning, which represents 40% more in relationship with the areas with burning. The accumulation of N in the root system was 70% higher in the larger rate in relation to the zero rate, being this amount a potential N source for the following cycle. Out of the total accumulated nitrogen 7 and 16% originated from the fertilizer, in function of the N-urea rates, with the use efficiency by the culture equivalent to 54%, regardless of the treatment

  6. Survival and development of Lymantria monacha (Lepidoptera: Lymantriidae) on North American and introduced Eurasian tree species.

    Science.gov (United States)

    Keena, M A

    2003-02-01

    Lymantria monacha (L.) (Lepidoptera: Lymantriidae), the nun moth, is a Eurasian pest of conifers that has potential for accidental introduction into North America. To project the potential host range of this insect if introduced into North America, survival and development of L. monacha on 26 North American and eight introduced Eurasian tree species were examined. Seven conifer species (Abies concolor, Picea abies, P. glauca, P. pungens, Pinus sylvestris with male cones, P. menziesii variety glance, and Tsuga canadensis) and six broadleaf species (Betula populifolia, Malus x domestica, Prunus serotiaa, Quercus lobata, Q. rubra, and Q. velutina) were suitable for L. monacha survival and development. Eleven of the host species tested were rated as intermediate in suitability, four conifer species (Larix occidentalis, P. nigra, P. ponderosa, P. strobus, and Pseudotsuga menziesii variety menziesii) and six broadleaf species (Carpinus caroliniana, Carya ovata, Fagus grandifolia, Populus grandidentata, Q. alba, and Tilia cordata) and the remaining 10 species tested were rated as poor (Acer rubrum, A. platanoidies, A. saccharum, F. americana, Juniperus virginiana, Larix kaempferi, Liriodendron tulipfera, Morus alba, P. taeda, and P. deltoides). The phenological state of the trees had a major impact on establishment, survival, and development of L. monacha on many of the tree species tested. Several of the deciduous tree species that are suitable for L. monacha also are suitable for L. dispar (L.) and L. mathura Moore. Establishment of L. monacha in North America would be catastrophic because of the large number of economically important tree species on which it can survive and develop, and the ability of mated females to fly and colonize new areas.

  7. A cross-site comparison of factors influencing soil nitrification rates in northeastern USA forested watersheds

    Science.gov (United States)

    Ross, D.S.; Wemple, B.C.; Jamison, A.E.; Fredriksen, G.; Shanley, J.B.; Lawrence, G.B.; Bailey, S.W.; Campbell, J.L.

    2009-01-01

    Elevated N deposition is continuing on many forested landscapes around the world and our understanding of ecosystem response is incomplete. Soil processes, especially nitrification, are critical. Many studies of soil N transformations have focused on identifying relationships within a single watershed but these results are often not transferable. We studied 10 small forested research watersheds in the northeastern USA to determine if there were common factors related to soil ammonification and nitrification. Vegetation varied between mixed northern hardwoods and mixed conifers. Watershed surface soils (Oa or A horizons) were sampled at grid or transect points and analyzed for a suite of chemical characteristics. At each sampling point, vegetation and topographic metrics (field and GIS-based) were also obtained. Results were examined by watershed averages (n = 10), seasonal/watershed averages (n = 28), and individual sampling points (n = 608). Using both linear and tree regression techniques, the proportion of conifer species was the single best predictor of nitrification rates, with lower rates at higher conifer dominance. Similar to other studies, the soil C/N ratio was also a good predictor and was well correlated with conifer dominance. Unlike other studies, the presence of Acer saccharum was not by itself a strong predictor, but was when combined with the presence of Betula alleghaniensis. Topographic metrics (slope, aspect, relative elevation, and the topographic index) were not related to N transformation rates across the watersheds. Although found to be significant in other studies, neither soil pH, Ca nor Al was related to nitrification. Results showed a strong relationship between dominant vegetation, soil C, and soil C/N. ?? 2008 Springer Science+Business Media, LLC.

  8. Predicting greenhouse gas emissions and soil carbon from changing pasture to an energy crop.

    Directory of Open Access Journals (Sweden)

    Benjamin D Duval

    Full Text Available Bioenergy related land use change would likely alter biogeochemical cycles and global greenhouse gas budgets. Energy cane (Saccharum officinarum L. is a sugarcane variety and an emerging biofuel feedstock for cellulosic bio-ethanol production. It has potential for high yields and can be grown on marginal land, which minimizes competition with grain and vegetable production. The DayCent biogeochemical model was parameterized to infer potential yields of energy cane and how changing land from grazed pasture to energy cane would affect greenhouse gas (CO2, CH4 and N2O fluxes and soil C pools. The model was used to simulate energy cane production on two soil types in central Florida, nutrient poor Spodosols and organic Histosols. Energy cane was productive on both soil types (yielding 46-76 Mg dry mass · ha(-1. Yields were maintained through three annual cropping cycles on Histosols but declined with each harvest on Spodosols. Overall, converting pasture to energy cane created a sink for GHGs on Spodosols and reduced the size of the GHG source on Histosols. This change was driven on both soil types by eliminating CH4 emissions from cattle and by the large increase in C uptake by greater biomass production in energy cane relative to pasture. However, the change from pasture to energy cane caused Histosols to lose 4493 g CO2 eq · m(-2 over 15 years of energy cane production. Cultivation of energy cane on former pasture on Spodosol soils in the southeast US has the potential for high biomass yield and the mitigation of GHG emissions.

  9. Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling.

    Science.gov (United States)

    Oloriz, María I; Gil, Víctor; Rojas, Luis; Portal, Orelvis; Izquierdo, Yovanny; Jiménez, Elio; Höfte, Monica

    2012-05-01

    Brown rust caused by the fungus Puccinia melanocephala is a major disease of sugarcane (Saccharum spp.). A sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety B4362, showed a post-haustorial hypersensitive response (HR)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to P. melanocephala via suppression subtractive hybridization (SSH). Tester cDNA was derived from the brown rust-resistant mutant after inoculation with P. melanocephala, while driver cDNAs were obtained from the non-inoculated resistant mutant and the inoculated susceptible donor variety B4362. Database comparisons of the sequences of the SSH recombinant clones revealed that, of a subset of 89 non-redundant sequences, 88% had similarity to known functional genes, while 12% were of unknown function. Thirteen genes were selected for transcript profiling in the resistant mutant and the susceptible donor variety. Genes involved in glycolysis and C4 carbon fixation were up-regulated in both interactions probably due to disturbance of sugarcane carbon metabolism by the pathogen. Genes related with the nascent polypeptide associated complex, post-translational proteome modulation and autophagy were transcribed at higher levels in the compatible interaction. Up-regulation of a putative L-isoaspartyl O-methyltransferase S-adenosylmethionine gene in the compatible interaction may point to fungal manipulation of the cytoplasmatic methionine cycle. Genes coding for a putative no apical meristem protein, S-adenosylmethionine decarboxylase, non-specific lipid transfer protein, and GDP-L-galactose phosphorylase involved in ascorbic acid biosynthesis were up-regulated in the incompatible interaction at the onset of haustorium formation, and may contribute to the HR-mediated defense response in the rust-resistant mutant.

  10. Large-Scale Variations in Lumber Value Recovery of Yellow Birch and Sugar Maple in Quebec, Canada.

    Science.gov (United States)

    Hassegawa, Mariana; Havreljuk, Filip; Ouimet, Rock; Auty, David; Pothier, David; Achim, Alexis

    2015-01-01

    Silvicultural restoration measures have been implemented in the northern hardwoods forests of southern Quebec, Canada, but their financial applicability is often hampered by the depleted state of the resource. To help identify sites most suited for the production of high quality timber, where the potential return on silvicultural investments should be the highest, this study assessed the impact of stand and site characteristics on timber quality in sugar maple (Acer saccharum Marsh.) and yellow birch (Betula alleghaniensis Britt.). For this purpose, lumber value recovery (LVR), an estimate of the summed value of boards contained in a unit volume of round wood, was used as an indicator of timber quality. Predictions of LVR were made for yellow birch and sugar maple trees contained in a network of more than 22000 temporary sample plots across the Province. Next, stand-level variables were selected and models to predict LVR were built using the boosted regression trees method. Finally, the occurrence of spatial clusters was verified by a hotspot analysis. Results showed that in both species LVR was positively correlated with the stand age and structural diversity index, and negatively correlated with the number of merchantable stems. Yellow birch had higher LVR in areas with shallower soils, whereas sugar maple had higher LVR in regions with deeper soils. The hotspot analysis indicated that clusters of high and low LVR exist across the province for both species. Although it remains uncertain to what extent the variability of LVR may result from variations in past management practices or in inherent site quality, we argue that efforts to produce high quality timber should be prioritized in sites where LVR is predicted to be the highest.

  11. Oral Triiodothyronine for Infants and Children Undergoing Cardiopulmonary Bypass.

    Science.gov (United States)

    Marwali, Eva M; Boom, Cindy E; Budiwardhana, Novik; Fakhri, Dicky; Roebiono, Poppy S; Santoso, Anwar; Sastroasmoro, Sudigdo; Slee, April; Portman, Michael A

    2017-08-01

    This study evaluated the efficacy and safety of oral triiodothyronine (T3; Tetronine, Dalim BioTech, Korea) for infants and children undergoing cardiopulmonary bypass in an Indonesian population. We performed a single-center, randomized, double-blind, and placebo-controlled trial in children aged 3 years and younger undergoing congenital heart disease operations with cardiopulmonary bypass. We administered oral T3 (1 μg/kg per body weight/dose) or placebo (saccharum lactis) by nasogastric tube every 6 hours for 60 hours after induction of anesthesia. The primary end point, time to extubation, was compared with Cox regression. The modified intention-to-treat group included 101 placebo and 104 treated patients. The stratified log-rank test did not show a significant treatment difference (p = 0.061) for time to extubation, but after adjustment for age, the nutritional Z score, and Aristotle surgical complexity, the hazard ratio was 1.33 (95% confidence interval, 1.00 to 1.76; p = 0.049). The effect of T3 was stronger in the strata aged 5 months and younger (hazard ratio, 1.86; 95% confidence interval 1.02 to 3.39; p = 0.043). Median intubation time was 47.3 hours for the placebo and 32.1 hours for the T3 group in aged 5 months and younger. Adverse events rates, including arrhythmia, were similar between groups, although sepsis was more frequent with placebo. Oral T3 supplementation may shorten time to extubation in children undergoing congenital heart disease operations, particularly infants aged 5 months or younger. Administration is relatively safe, simple and inexpensive. Copyright © 2017 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  12. Demanda de mano de obra y rentabilidad de bancos forrajeros en Esparza, Costa Rica

    Directory of Open Access Journals (Sweden)

    Luz Yaneth Sánchez Sarmiento

    2010-07-01

    Full Text Available En Esparza (Costa Rica se evaluó la viabilidad de invertir en bancos forrajeros (BF como alternativa de suplementación para vacas en sistema doble propósito durante la época seca (125 días. Se usaron como indicadores de rentabilidad el Valor Presente Neto (VPN y la Tasa Interna de Retorno (TIR. Se realizó un análisis de sensibilidad para el incremento del precio de la leche, los concentrados, la gallinaza y la mano de obra. Los modelos se elaboraron comparando los BF de plantas leñosas perennes de corte y acarreo de Cratylia argentea + caña de azúcar (Saccharum officinarum (BFC y de ramoneo Leucaena leucocephala (BFL vs. suplementos comerciales tipos concentrado y gallinaza. En los BFC se requirieron 71.1 jornales/año y el análisis financiero indicó que son rentables al compararlos con la gallinaza (VPN = US$362.2, TIR = 17% y concentrados (VPN = US$1953.9; TIR=39%. En BFL se requirieron 13 jornales/año y fueron igualmente rentables en comparación con gallinaza (VPN = US$1594.7; TIR = 21% y con concentrados (VPN = US$3619.6; TIR=35%. Los modelos fueron sensibles a la producción y cambios de precios en la leche, gallinaza, concentrados y el valor del jornal. Los BF son una buena alternativa para la suplementación de ganado en la época seca y una importante fuente de empleo en la región; sin embargo, los altos costos de la mano de obra contratada pueden limitar la adopción.

  13. Carbon stock and humification index of organic matter affected by sugarcane straw and soil management

    Directory of Open Access Journals (Sweden)

    Aline Segnini

    2013-10-01

    Full Text Available The maintenance of sugarcane (Saccharum spp. straw on a soil surface increases the soil carbon (C stocks, but at lower rates than expected. This fact is probably associated with the soil management adopted during sugarcane replanting. This study aimed to assess the impact on soil C stocks and the humification index of soil organic matter (SOM of adopting no-tillage (NT and conventional tillage (CT for sugarcane replanting. A greater C content and stock was observed in the NT area, but only in the 0-5 cm soil layer (p < 0.05. Greater soil C stock (0-60 cm was found in soil under NT, when compared to CT and the baseline. While C stock of 116 Mg ha-1 was found in the baseline area, in areas under CT and NT systems the values ranged from 120 to 127 Mg ha-1. Carbon retention rates of 0.67 and 1.63 Mg C ha-1 year-1 were obtained in areas under CT and NT, respectively. Laser-Induced Fluorescence Spectroscopy showed that CT makes the soil surface (0-20 cm more homogeneous than the NT system due to the effect of soil disturbance, and that the SOM humification index (H LIF is larger in CT compared to NT conditions. In contrast, NT had a gradient of increasing H LIF, showing that the entry of labile organic material such as straw is also responsible for the accumulation of C in this system. The maintenance of straw on the soil surface and the adoption of NT during sugarcane planting are strategies that can increase soil C sequestration in the Brazilian sugarcane sector.

  14. Relative Preference and Localized Food Affect Predator Space Use and Consumption of Incidental Prey.

    Directory of Open Access Journals (Sweden)

    Tyler E Schartel

    Full Text Available Abundant, localized foods can concentrate predators and their foraging efforts, thus altering both the spatial distribution of predation risk and predator preferences for prey that are encountered incidentally. However, few investigations have quantified the spatial scale over which localized foods affect predator foraging behavior and consumption of incidental prey. In spring 2010, we experimentally tested how point-source foods altered how generalist predators (white-footed mice, Peromyscus leucopus utilized space and depredated two incidental prey items: almonds (Prunus dulcis; highly profitable and maple seeds (Acer saccharum; less profitable. We estimated mouse population densities with trapping webs, quantified mouse consumption rates of these incidental prey items, and measured local mouse activity with track plates. We predicted that 1 mouse activity would be elevated near full feeders, but depressed at intermediate distances from the feeder, 2 consumption of both incidental prey would be high near feeders providing less-preferred food and, 3 consumption of incidental prey would be contingent on predator preference for prey relative to feeders providing more-preferred food. Mouse densities increased significantly from pre- to post-experiment. Mean mouse activity was unexpectedly greatest in control treatments, particularly <15 m from the control (empty feeder. Feeders with highly preferred food (sunflower seeds created localized refuges for incidental prey at intermediate distances (15 to 25m from the feeder. Feeders with less-preferred food (corn generated localized high risk for highly preferred almonds <10 m of the feeder. Our findings highlight the contingent but predictable effects of locally abundant food on risk experienced by incidental prey, which can be positive or negative depending on both spatial proximity and relative preference.

  15. Comparison of carbon sequestration potential in agricultural and afforestation farming systems

    Directory of Open Access Journals (Sweden)

    Chinsu Lin

    2013-04-01

    Full Text Available In the last few decades, many forests have been cut down to make room for cultivation and to increase food or energy crops production in developing countries. In this study, carbon sequestration and wood production were evaluated on afforested farms by integrating the Gaussian diameter distribution model and exponential diameter-height model derived from sample plots of an afforested hardwood forest in Taiwan. The quantity of sequestrated carbon was determined based on aboveground biomass. Through pilot tests run on an age-volume model, an estimation bias was obtained and used to correct predicted volume estimates for a farm forest over a 20-year period. An estimated carbon sequestration of 11,254 t C was observed for a 189ha-hardwood forest which is equivalent to 41,264 t CO2. If this amount of carbon dioxide were exchanged on the Chicago Climate Exchange (CCX market, the income earned would be 821 US$ ha- 1. Carbon sequestration from rice (Oryza sativa or sugarcane (Saccharum officinarum production is discharged as a result of straw decomposition in the soil which also improves soil quality. Sugarcane production does not contribute significantly to carbon sequestration, because almost all the cane fiber is used as fuel for sugar mills. As a result of changing the farming systems to hardwood forest in this study area, carbon sequestration and carbon storage have increased at the rate of 2.98 t C ha- 1 year- 1. Net present value of afforestation for a 20-year period of carbon or wood management is estimated at around US$ 30,000 given an annual base interest rate of 3 %.

  16. EFFICIENCY OF PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR IN SUGARCANE

    Directory of Open Access Journals (Sweden)

    Antonio Morgado González

    2015-10-01

    Full Text Available Plant growth promoting rhizobacteria (PGPR are an alternative for promoting sugarcane (Saccharum spp. development. Growth promotion was evaluated in sugarcane vitroplants inoculated separately with twenty-four strains of seven different bacterial species. Total indole synthesis and phosphate solubilization activity were determined in each strain. The experimental unit was one 5 L pot filled with a sterile mixture of farm soil-agrolite and one plant. The experimental design was completely random. Inoculation consisted of 1.0 mL of bacterial suspension (1 × 107 CFU. Plant height, stem diameter, number of shoots, leaf area and dry matter of shoot and root were determined every two weeks. The Ochrobactrum anthropi strains N208 and IMP311 and Pseudomonas luteola IMPCA244 had the highest production of total indoles (116.69, 115.70 and 117.34 µg mL-1, respectively. The Stenotrophomonas maltophilia strains CA158 and 79 exhibited the highest values of phosphate solubilization (222.43 and 216.38 µg mL-1, respectively. In general, plant height increased 27.75%, stem diameter 30.75%, number of tillers 38.5%, leaf area 49%, aerial dry matter 59.75% and root dry matter 59.5%. P. luteola, P. f luorescens, O. anthropi and S. maltophilia exhibited the highest values of the leaf area index, net assimilation, and relative and absolute growth rates. P. luteola IMPCA244, O. anthropi IMP311, Aeromonas salmonicida N264, Burkholderia cepacia N172, P. f luorescens N50 and S. maltophilia 79 promoted the highest values in different response variables throughout the study. Before using these strains as sugarcane biofertilizer, additional studies are required.

  17. Demanda de mano de obra y rentabilidad de bancos forrajeros en Esparza, Costa Rica

    Directory of Open Access Journals (Sweden)

    Sánchez Sarmiento Luz Yaneth

    2010-09-01

    Full Text Available En Esparza (Costa Rica se evaluó la viabilidad de invertir en bancos forrajeros (BF como alternativa de suplementación para vacas en sistema doble propósito durante la época seca (125 días. Se usaron como indicadores de rentabilidad el Valor Presente Neto (VPN y la Tasa Interna de Retorno (TIR. Se realizó un análisis de sensibilidad para el incremento del precio de la leche, los concentrados, la gallinaza y la mano de obra. Los modelos se elaboraron comparando los BF de plantas leñosas perennes de corte y acarreo de Cratylia argentea + caña de azúcar (Saccharum officinarum (BFC y de ramoneo Leucaena leucocephala (BFL vs. suplementos comerciales tipos concentrado y gallinaza. En los BFC se requirieron 71.1 jornales/año y el análisis financiero indicó que son rentables al compararlos con la gallinaza (VPN = US$362.2, TIR = 17% y concentrados (VPN = US$1953.9; TIR=39%. En BFL se requirieron 13 jornales/año y fueron igualmente rentables en comparación con gallinaza (VPN = US$1594.7; TIR = 21% y con concentrados (VPN = US$3619.6; TIR=35%. Los modelos fueron sensibles a la producción y cambios de precios en la leche, gallinaza, concentrados y el valor del jornal. Los BF son una buena alternativa para la suplementación de ganado en la época seca y una importante fuente de empleo en la región; sin embargo, los altos costos de la mano de obra contratada pueden limitar la adopción.

  18. Incorporating interspecific competition into species-distribution mapping by upward scaling of small-scale model projections to the landscape.

    Directory of Open Access Journals (Sweden)

    Mark Baah-Acheamfour

    Full Text Available There are a number of overarching questions and debate in the scientific community concerning the importance of biotic interactions in species distribution models at large spatial scales. In this paper, we present a framework for revising the potential distribution of tree species native to the Western Ecoregion of Nova Scotia, Canada, by integrating the long-term effects of interspecific competition into an existing abiotic-factor-based definition of potential species distribution (PSD. The PSD model is developed by combining spatially explicit data of individualistic species' response to normalized incident photosynthetically active radiation, soil water content, and growing degree days. A revised PSD model adds biomass output simulated over a 100-year timeframe with a robust forest gap model and scaled up to the landscape using a forestland classification technique. To demonstrate the method, we applied the calculation to the natural range of 16 target tree species as found in 1,240 provincial forest-inventory plots. The revised PSD model, with the long-term effects of interspecific competition accounted for, predicted that eastern hemlock (Tsuga canadensis, American beech (Fagus grandifolia, white birch (Betula papyrifera, red oak (Quercus rubra, sugar maple (Acer saccharum, and trembling aspen (Populus tremuloides would experience a significant decline in their original distribution compared with balsam fir (Abies balsamea, black spruce (Picea mariana, red spruce (Picea rubens, red maple (Acer rubrum L., and yellow birch (Betula alleghaniensis. True model accuracy improved from 64.2% with original PSD evaluations to 81.7% with revised PSD. Kappa statistics slightly increased from 0.26 (fair to 0.41 (moderate for original and revised PSDs, respectively.

  19. Photosynthetic and growth response of sugar maple (Acer saccharum Marsh.) mature trees and seedlings to calcium, magnesium, and nitrogen additions in the Catskill Mountains, NY, USA

    Science.gov (United States)

    Momen, Bahram; Behling, Shawna J; Lawrence, Gregory B.; Sullivan, Joseph H

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the forest floor, which can impede seedling survival.

  20. Photosynthetic and Growth Response of Sugar Maple (Acer saccharum Marsh.) Mature Trees and Seedlings to Calcium, Magnesium, and Nitrogen Additions in the Catskill Mountains, NY, USA.

    Science.gov (United States)

    Momen, Bahram; Behling, Shawna J; Lawrence, Greg B; Sullivan, Joseph H

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the forest floor, which can impede seedling survival.

  1. Diversidade de fungos em um vertissolo com adição de vinhaça na cultura de cana-de-açúcar ( saccharum officinarum l.)

    OpenAIRE

    Barros, Rubens Pessoa de; UNIVERSIDADE ESTADUAL DE ALAGOAS

    2012-01-01

    O artigo trata do estudo da aplicação da vinhaça em solos cultivados com cana-de-açúcar (S. officinarum L.). O presente trabalho teve como objetivo estudar o efeito da aplicação de vinhaça em solo cultivado com cana-de-açúcar sobre a densidade e diversidade de fungos presentes no solo e compará-lo com a área sem aplicação de vinhaça, em duas áreas distintas em canaviais pertencentes à Usina São José do Pinheiro, no município de Laranjeiras, Estado de Sergipe, Brasil. Área A – Fazenda Poção co...

  2. Predição do consumo de pasto de capim-elefante (Pennisetum purpureum, Schumack por vacas mestiças Holandês x Zebu em lactação Prediction of the voluntary intake of elephantgrass (Pennisetum purpureum, Schumack grazing by Holstein x Zebu lactating dairy cows

    Directory of Open Access Journals (Sweden)

    Fernando César Ferraz Lopes

    2005-06-01

    Full Text Available Foram desenvolvidas equações de predição de consumo de pasto de capim-elefante (Pennisetum purpureum, Schumack por vacas mestiças Holandês x Zebu em lactação, utilizando-se procedimentos de stepwise em regressões múltiplas, aplicados a um banco de dados de experimentos conduzidos ao longo de três anos na Embrapa Gado de Leite (Coronel Pacheco, MG. As variáveis independentes disponíveis foram relacionadas a características inerentes às vacas (dias em lactação; teores de proteína, gordura e extrato seco total e produções destes componentes no leite; produção de leite in natura ou corrigida para 4% de gordura; ordem de lactação; peso vivo atual; peso vivo ao parto e grau de sangue Holandês x Zebu; ao manejo (dias de pastejo; disponibilidade de forragem e período de descanso da pastagem; ao ambiente (estação do ano e precipitação pluviométrica e à alimentação (digestibilidade in vitro e parâmetros da composição química do pasto de capim-elefante e da cana-de-açúcar - Saccharum officinarum (L. corrigida com 1% de uréia; consumos de suplemento volumoso (cana corrigida com uréia e concentrado; concentrações fecais de proteína bruta e de fibras em detergente neutro e ácido. Efeitos linear e quadrático e transformações logarítmicas foram adicionalmente incluídos no banco de dados. Foram obtidas equações de predição de consumo de pasto de capim-elefante (expresso em kg/vaca/dia ou % do peso vivo com coeficientes de determinação de 65,2 a 67,0%. As principais variáveis independentes incluídas nas equações foram o consumo do suplemento volumoso usado na estação seca do ano (cana corrigida com uréia; a digestibilidade in vitro do pasto de capim-elefante; a precipitação pluviométrica; a produção de leite corrigida para 4% de gordura; o peso vivo atual ou, em alternativa a este, o valor da pesagem realizada após o parto da vaca; além do consumo de suplemento concentrado, que evidenciou um

  3. Aditivos químicos ou biológicos na ensilagem de cana-de-açúcar: 2. parâmetros ruminais e degradabilidade da matéria seca e das frações fibrosas Chemical and biological additives in sugar cane silages: 2. ruminal parameters and DM and fiber degradabilities

    Directory of Open Access Journals (Sweden)

    Patrick Schmidt

    2007-10-01

    Full Text Available Objetivou-se avaliar o efeito da inclusão de aditivos na ensilagem de cana-de-açúcar (Saccharum officinarum L. sobre a degradação de MS e de componentes da parede celular e sobre os parâmetros de fermentação ruminal em bovinos alimentados com dietas contendo essas silagens. Utilizaram-se cinco novilhos da raça Nelore providos de cânula ruminal, alocados em delineamento quadrado latino 5 ´ 5 e alimentados com dietas com 65% de volumoso (% MS. Foram avaliadas cinco silagens (base úmida: controle - cana-de-açúcar, sem aditivos; uréia - cana-de-açúcar + 0,5% ureia; benzoato - cana-de-açúcar + 0,1% de benzoato de sódio; LP - cana-de-açúcar inoculada com Lactobacillus plantarum (1 ´ 10(6 ufc/g MV; LB - cana-de-açúcar inoculada com L. buchneri (3,6 ´ 10(5 ufc/g forragem. A forragem foi armazenada em silos do tipo poço por 90 dias antes do fornecimento aos animais. Os parâmetros ruminais foram afetados de forma moderada pelas silagens e tiveram forte efeito do horário de coleta de amostras. As concentrações molares médias dos ácidos acético, propiônico e butírico foram de 60,9; 19,3 e 10,2 mM, respectivamente. O ambiente ruminal proporcionado por dietas formuladas com silagens de cana-de-açúcar foi satisfatório e similar ao tradicionalmente observado em dietas contendo cana. O uso de aditivos na ensilagem influenciou, de forma não-significativa, a degradabilidade ruminal da MS e da MO, mas não alterou a degradabilidade ruminal da fração fibrosa. Os aditivos aplicados à cana-de-açúcar resultaram em pequenas alterações na maior parte das variáveis avaliadas. Apesar de a degradabilidade ruminal das silagens ter sido pouco afetada pelo uso de aditivos, os valores observados foram próximos aos observados para a cana-de-açúcar in natura.The objective was to evaluate the addition of additives in the ensiling of sugar cane (Saccharum officinarum L. on the degradation of DM and components of cellular wall and

  4. Nitrogen fertilizer leaching in an Oxisol cultivated with sugarcane Nitrogênio lixiviado num Latossolo cultivado com cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Pablo Javier Ghiberto

    2011-02-01

    Full Text Available Nitrogen (N leaching below the crop-rooting zone represents not only a valuable loss of nutrients for the plant, but also a potential pollution source of groundwater. The objective of this work was to quantify leaching losses of native N and that derived from fertilizer in an Oxisol that was cultivated with sugarcane (Saccharum officinarum during the crop plant cycle. The sugarcane was planted and fertilized with urea in the planting furrow, with 120 kg ha-1 of N. In order to determine the fate of the fertilizer - N, four microplots with 15N enriched fertilizer were installed. Input and output of N at the depth of 0.9 m were quantified from the flux density of water and the N concentration in soil solution. During the evaluation period the rainfall was 141 mm less than the historical average (1,315 mm, and the climate was drier than normal in January. The average concentration of mineral N in soil solution was 1.8 mg L-1. The abundance of 15N was very high at the beginning (first week of the assessment period and remained approximately constant (0.453 atom% of 15N until the end of the period. The internal drainage was 91 mm of water and the N leaching loss was 1.1 kg ha-1 of N, with only 54 g ha-1 derived from fertilizer. Therefore, under high demand of N by the crop in a system without burning before planting, the leaching of N was not considerable, mainly because the surplus of water between the months of December and March was lower than expected and also because the extraction of nitrogen by the crop was high.A lixiviação de nitrogênio (N abaixo da zona radicular representa uma valiosa perda do nutriente para as plantas e uma fonte potencial de poluição do lençol freático. Quantificaram-se as perdas de N por lixiviação num Latossolo Vermelho Amarelo cultivado com cana-de-açúcar (Saccharum officinarum durante o ciclo agrícola de cana-planta. A cultura foi implantada e fertilizada no sulco com 120 kg ha-1 de N-uréia. Para conhecer

  5. Efecto de la edad de la planta y de diferentes tratamientos fotoinductivos en la floración de la caña de azúcar Saccharum sp.

    Directory of Open Access Journals (Sweden)

    Viveros V. Carlos A.

    1991-12-01

    Full Text Available La floración y la posterior polinización para la formación de la semilla, es el mecanismo para poder reunir en un solo genotipo las características deseables de una planta. La no floración de un alto número de variedades de caña de azúcar en condiciones de fotoperiodo natural del Valle del Cauca, restringe el uso de la totalidad del potencial genético existente en el banco de germoplasma de CENICANA. Se estudió el efecto de la edad de la planta y de varios tratamientos en la inducción de floración de la caña de azúcar. Los experimentos se realizaron durante 1990 en la casa de fotoperíodo de la Estación Experimental San Antonio, Centro de Investigación de la Caña de Azúcar de Colombia. La siembra de la variedad Mex 64-1487 se realizó en recipientes plásticos de 34 litros de capacidad cuando las plantas de la variedad tuvieron tres, cuatro, cinco y seis meses de edad recibieron tratamiento adicional de la luz artificial a partir del primero de julio, ajustándolo a una curva de fotoperiodo de 140 de latitud. A partir de los tres meses, cuando las plantas tenían ocho hojas formadas, estaban en capacidad de ser inducidas a florecer con tratamiento de luz artificial. Las plantas de mayor edad también florecieron en su totalidad y en forma simultánea, alrededor de los 174 días después de iniciar el tratamiento de luz. Se realizó un segundo experimento con la variedad Mex 64-1487 dándole seis tratamientos foto inducidos de trece horas, desde 1 hasta 60 días, con disminución de luz de 2 minutos cada dos días. Se encontró que un solo día de 13 horas con descenso hasta 12 horas fue suficiente para inducir la floración.Flowering and pollination to produce seeds is the mechanism which assembles in a single genotype the desirable characteristics of a plant. No flowering of a high number of varieties under natural condition in the Valle del Cauca restricts the use of the whole genetic potential existing in CENICANA's germplasm bank. It was studied the effect of plant age and several photoinductive treatments in flower induction of sugarcane. The experiments were carried out during 1990 in San Antonio Experiment Station photoperiod house, Centro de Investigación de la Caña de Azúcar de Colombia. Planting of the variety Mex 64-1487 was done in 34 liters plastic pots. When the plants were three, four, five, and six months old were supplied with additional artifitial light starting july first following the photoperiod curve of 14o latitude. It was found that all the plants flower even those that have the light treatment when were three months old. At that age the plants have 8 leaves. AlI the plants flower around 174 days from the day they start getting the artifitial light. A second experiments was set up using Mex 64-1487 with 6 photoinductive treatments of thirteen hours during 1 to 60 days. The hours of light was decreased by two minutes every other day. It was found that one day with 13 light hours was enough to induce flower.

  6. Sustainability Issues and Opportunities in the Sugar and Sugar-Bioproduct Industries

    Directory of Open Access Journals (Sweden)

    Gillian Eggleston

    2015-09-01

    Full Text Available Like many other industries, the sugar and sugar-bioproduct industries are facing important sustainability issues and opportunities. The relatively low and fluctuating profit for sugar, surpluses of sugar, world-wide trend to produce alternative, renewable bio-based fuels and chemicals to those derived from petroleum and reduce greenhouse gases, water- and energy-intensive factories and refineries, and increased consumer demands for sustainably manufactured products are putting pressure on the industries to diversify for sustainability. Sugar crops, including sugar and energy cane (Saccharum officinarum, sugar and energy beets (Beta vulgaris, and sweet sorghum (Sorghum bicolor L. Moench, are excellent, renewable biomass feedstocks because of their availability, their being amongst the plants that give the highest yields of carbohydrates per hectare, and high sugar contents. While much research has been focused on conversion technologies for advanced biofuels and bioproducts, attention is now focused on developing sustainable supply chains of sugar feedstocks for the new, flexible biorefineries, with customers wanting maximum feedstock reliability and quality, while minimizing cost. All biomass from sugar crops are potential feedstocks. The cogeneration of bioelectricity from bagasse and leaf residues is being increasingly manufactured in more countries and, due to the high carbon content of bagasse and leaves, can also be converted into value-added products such as biochar. Sugar crops are superior feedstocks for the production of platform chemicals for the manufacture of a range of end-products, e.g., bioplastics, chemicals, and biomaterials. In several countries and regions, green sustainability criteria are now in place and have to be met to count against national biofuel targets. Processes to convert high-fiber sugar crop biomass into biofuel have been developed but there has only been limited commercialization at the large-scale.

  7. Pérdidas de suelo y nutrientes bajo diferentes coberturas vegetales en la zona Andina de Colombia

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    J. Alexander Rodríguez

    2009-07-01

    Full Text Available El estudio se realizó en la reserva natural El Ciprés, ubicada en la vereda Bellavista, municipio de El Dovio (Valle del Cauca. La vereda se encuentra en la zona Andina de la cordillera occidental, en el departamento del Valle del Cauca, entre 1700 y 1800 m.s.n.m., con una temperatura promedio de 18 °C. Según la clasificación climática de Holdridge, corresponde a una zona de bosque húmedo Montano Bajo, con una precipitación promedio entre 2500 y 2700 mm/año, una humedad relativa de 90% y una pendiente del suelo de 62%. Las mediciones se hicieron en parcelas de escorrentía de 32 m² cada una y siete tipos de coberturas: guadua (Guadua angustifolia Kunth, bosque secundario, pastura (Brachiaria decumbens, café (Coffea arabica; banco de proteína (Trichanthera gigantea, caña forrajera (Saccharum officinarum y cultivo limpio conformado por yuca (Manihot esculenta, maíz (Zea mays y arracacha (Arracacia zanthorrhiza Brancroft. Para la evaluación se midieron las pérdidas de suelo y los nutrientes calcio, magnesio, potasio y fósforo en un periodo de 7 meses. Los resultados mostraron diferencias (P < 0.05 entre las coberturas evaluadas. Las pérdidas más altas de suelo y nutrientes ocurrieron bajo cultivos limpios (2.61 t/ha y las más bajas en guadua (0.08 t/ha. Estos resultados sugieren ventajas de los bosques de guadua en esta zona de Colombia, ya que además de contribuir a la conservación de los suelos y al mantenimiento de la fertilidad, es una alternativa económica complementaria para los productores rurales a partir de su manejo y aprovechamiento.

  8. Century-scale Variations in Plant and Soil Nitrogen Pools and Isotopic Composition in Northern Hardwood Forests

    Science.gov (United States)

    Goodale, C. L.; Fuss, C. B.; Lang, A.; Ollinger, S. V.; Ouimette, A.; Vadeboncoeur, M. A.; Zhou, Z.; Lovett, G. M.

    2017-12-01

    The mineral soil may act as both a source and a sink of nitrogen to plants over decadal to centennial timescales. However, the enormous size and spatial heterogeneity of mineral soil N regularly impede study of its role over the course of forest succession. Here, we measured tree and soil stocks of C, N and 15N to 50 cm depth in and near Hubbard Brook, New Hampshire, across eight forest stands of varying time since harvest (two stands each of 20, 40, and 100 years post-harvest, and old-growth forest). Measurements show that tree biomass and N stocks increased with stand age to an average of 145 t C/ha and 556 kg N/ha in old-growth forests, as cumulative net growth and N increment rates decreased from young (20 and 40-year old) to mature (100-year) to old-growth stands. Plant %N varied more by site than species, while plant 15N varied more by tree species than by site. Of the most common species, Acer saccharum (sugar maple) had consistently lighter 15N in all tissues (bark, leaf, wood) than Betula alleghaniensis (yellow birch). Soil organic matter stocks are very large, averaging 154 t C/ha and 8.1 tN/ha to 50 cm depth. Neither C nor N stock varied regularly with stand age, but old-growth stands had lower C:N ratios and higher 15N values than the successional stands. Ongoing analysis will predict the effects of harvest, regrowth, and N inputs and losses on expected and observed 15N changes over succession. These observations support the great capacity of the mineral soil to store and potentially supply N to northern hardwood forests.

  9. Comparative anthelmintic activity investigation of selected ethno-medicinal weeds

    Science.gov (United States)

    Pueblos, Kirstin Rhys S.; Bajalla, Mark; Pacheco, Dixie; Ganot, Sheila; Paig, Daisy; Tapales, Radyn; Lagare, Jeanne; Quimque, Mark Tristan J.

    2017-01-01

    Helminth infections are one of the seriously neglected potent diseases in many parts of the world. The problems of parasitic helminthes becoming resistant to currently available anthelmintic drugs pose a challenge for the search - relying on natural products - for new and better anthelmintics. In this paper, four abundant Philippine weeds: Chrysopogon aciculatus Trin. Cyperus brevifolius Rottb., Ruellia tuberosa Linn. and Saccharum spontaneum Linn. were investigated for their anthelmintic activities to establish basis of their folkloric claim. The hexane-soluble and chloroform-soluble extracts were obtained through sequential solvent partitioning of the crude ethanolic extract of the air-dried aerial part of each plant sample. Meanwhile, the decoction was obtained from fresh aerial part of the plant samples. All extracts were then subjected to in vitro anthelmintic screening at different concentration as per method of Ghosh, et al. against African nightcrawler earthworms (Eudrillus euginiae) in which the activity of the extracts was determined by correlation with time. The anthelmintic bioassay results revealed a dose-dependent toxicity relationship. It indicated relatively low anthelmintic activities of the decoction of the four plant samples as compared to their corresponding crude ethanol extracts. Among the crude ethanol extracts, C. brevifolius (CBE) gave fastest time to bring about paralysis and death to the test organisms at all concentrations tested. For the hexane extracts, R. tuberosa (RTH) gave better activity among other plant samples. Lastly, among the chloroform-soluble extracts, both that of C. brevifolius (CBC) and R. tuberosa (RTC) comparably showed strongest anthelmintic activities at all tested concentrations, thus, exhibited best anthelmintic activity that is remarkably comparable to the positive control, Mebendazole at the highest concentration tested. In fact, CBC and RTC showed highest anthelmintic potential compared to all extracts tested in

  10. Unraveling the genetic structure of Brazilian commercial sugarcane cultivars through microsatellite markers.

    Science.gov (United States)

    Manechini, João Ricardo Vieira; da Costa, Juliana Borges; Pereira, Bruna Turcatto; Carlini-Garcia, Luciana Aparecida; Xavier, Mauro Alexandre; Landell, Marcos Guimarães de Andrade; Pinto, Luciana Rossini

    2018-01-01

    The Brazilian sugarcane industry plays an important role in the worldwide supply of sugar and ethanol. Investigation into the genetic structure of current commercial cultivars and comparisons to the main ancestor species allow sugarcane breeding programs to better manage crosses and germplasm banks as well as to promote its rational use. In the present study, the genetic structure of a group of Brazilian cultivars currently grown by commercial producers was assessed through microsatellite markers and contrasted with a group of basic germplasm mainly composed of Saccharum officinarum and S. spontaneum accessions. A total of 285 alleles was obtained by a set of 12 SSRs primer pairs that taken together were able to efficiently distinguish and capture the genetic variability of sugarcane commercial cultivars and basic germplasm accessions allowing its application in a fast and cost-effective way for routine cultivar identification and management of sugarcane germplasm banks. Allelic distribution revealed that 97.6% of the cultivar alleles were found in the basic germplasm while 42% of the basic germplasm alleles were absent in cultivars. Of the absent alleles, 3% was exclusive to S. officinarum, 33% to S. spontaneum and 19% to other species/exotic hybrids. We found strong genetic differentiation between the Brazilian commercial cultivars and the two main species (S. officinarum: [Formula: see text] = 0.211 and S. spontaneum: [Formula: see text] = 0.216, Pcommercial cultivars. Average dissimilarity within cultivars was 1.2 and 1.4 times lower than that within S. officinarum and S. spontaneum. Genetic divergence found between cultivars and S. spontaneum accessions has practical applications for energy cane breeding programs as the choice of more divergent parents will maximize the frequency of transgressive individuals in the progeny.

  11. Projected change in climate thresholds in the Northeastern U.S.: implications for crops, pests, livestock, and farmers

    Energy Technology Data Exchange (ETDEWEB)

    Chase, L. [Department of Animal Science, Cornell University, Ithaca, NY (United States); Hayhoe, K. [Department of Geosciences, Texas Tech University, Lubbock, TX (United States); Petzoldt, C. [Integrated Pest Management Program, Cornell University, Geneva, NY (United States); Seaman, A. [Integrated Pest Management Program, Cornell University, Geneva, NY (United States); Wolfe, D. W. [Department of Horticulture, Cornell University, Ithaca, NY (United States)

    2008-07-01

    Most prior climate change assessments for U.S. agriculture have focused on major world food crops such as wheat and maize. While useful from a national and global perspective, these results are not particularly relevant to the Northeastern U.S. agriculture economy, which is dominated by dairy milk production, and high-value horticultural crops such as apples (Malus domestica), grapes (vitis vinifera), sweet corn (Zea mays var. rugosa), cabbage (Brassica oleracea var. capitata), and maple syrup (sugar maple, Acer saccharum). We used statistically downscaled climate projections generated by the HadCM3 atmosphere-ocean general circulation model, run with Intergovernmental Panel on Climate Change future emissions scenarios Alfi (higher) and B1 (lower), to evaluate several climate thresholds of direct relevance to agriculture in the region. A longer (frostfree) growing season could create new opportunities for farmers with enough capital to take risks on new crops (assuming a market for new crops can be developed). However, our results indicate that many crops will have yield losses associated with increased frequency of high temperature stress, inadequate winter chill period for optimum fruiting in spring, increased pressure from marginally over-wintering and/or invasive weeds, insects, or disease, or other factors. Weeds are likely to benefit more than cash crops from increasing atmospheric carbon dioxide. Projections of thermal heat index values for dairy cows indicate a substantial potential negative impact on milk production. At the higher compared to lower emissions scenario, negative climate change effects will occur sooner, and impact a larger geographic area within the region. Farmer adaptations to climate change will not be cost- or risk-free, and the impact on individual farm families and rural communities will depend on commodity produced, available capital, and timely, accurate climate projections.

  12. Large-Scale Variations in Lumber Value Recovery of Yellow Birch and Sugar Maple in Quebec, Canada.

    Directory of Open Access Journals (Sweden)

    Mariana Hassegawa

    Full Text Available Silvicultural restoration measures have been implemented in the northern hardwoods forests of southern Quebec, Canada, but their financial applicability is often hampered by the depleted state of the resource. To help identify sites most suited for the production of high quality timber, where the potential return on silvicultural investments should be the highest, this study assessed the impact of stand and site characteristics on timber quality in sugar maple (Acer saccharum Marsh. and yellow birch (Betula alleghaniensis Britt.. For this purpose, lumber value recovery (LVR, an estimate of the summed value of boards contained in a unit volume of round wood, was used as an indicator of timber quality. Predictions of LVR were made for yellow birch and sugar maple trees contained in a network of more than 22000 temporary sample plots across the Province. Next, stand-level variables were selected and models to predict LVR were built using the boosted regression trees method. Finally, the occurrence of spatial clusters was verified by a hotspot analysis. Results showed that in both species LVR was positively correlated with the stand age and structural diversity index, and negatively correlated with the number of merchantable stems. Yellow birch had higher LVR in areas with shallower soils, whereas sugar maple had higher LVR in regions with deeper soils. The hotspot analysis indicated that clusters of high and low LVR exist across the province for both species. Although it remains uncertain to what extent the variability of LVR may result from variations in past management practices or in inherent site quality, we argue that efforts to produce high quality timber should be prioritized in sites where LVR is predicted to be the highest.

  13. Leaf cuticles as mediators of environmental influences: new developments in the use of isolated cuticles

    International Nuclear Information System (INIS)

    Scherbatskoy, T.

    1994-01-01

    Isolated leaf cuticles have been used in our research to characterize trans-cuticular ion diffusion rates under various environmental treatments and to measure cuticular attenuation of solar radiation. These studies have been conducted to understand better the role of the leaf cuticle as a protective barrier against potential environmental stressors including acid rain, ozone and ultraviolet radiation. These studies provide examples of a variety of current research uses for isolated leaf cuticles. Ion permeability coefficients and exchange rates in isolated cuticles of Acer, Prunus and Citrus species have been studied using several experimental approaches, including measurement of adsorption kinetics, electrical potential and conductance, and perfusion rates. Measured electrical (diffusion) potentials under KCl gradients across isolated cuticles are positive, indicating greater cation permeability. Electrical potentials (and permeability coefficients) vary with ionic strength and pH, and affect the driving force for ion diffusion through cuticles. Ion permeability in cuticles of Prunus serotina foliage was affected by experimental exposure to ozone and ultraviolet treatments. These studies indicate that cuticle permeability properties can be significantly altered by environmental factors. These and related studies on the ion exchange kinetics of Acer saccharum leaf cuticles suggest that foliar ''leaching'' is dominated by cuticle surface exchange mechanisms, with the magnitude of cuticular ion permeation being relatively small. In working with various hardwood species, we observed that the success rate for cuticle isolation varies with tree species and time of year. Scanning electron micrographs of inner cuticle surfaces indicate that the effects of enzymatic digestion could vary with exposure time, possibly affecting transport properties of isolated cuticles. Experimental work to test this in Citrus, however, showed no significant effect of isolation time on

  14. Thirty-two years of change in an old-growth Ohio beech-maple forest.

    Science.gov (United States)

    Runkle, James R

    2013-05-01

    Old-growth forests dominated by understory-tolerant tree species are among forest types most likely to be in equilibrium. However, documentation of the degree to which they are in equilibrium over decades-long time periods is lacking. Changes in climate, pathogens, and land use all are likely to impact stand characteristics and species composition, even in these forests. Here, 32 years of vegetation changes in an old-growth beech (Fagus grandifolia)-sugar maple (Acer saccharum) forest in Hueston Woods, southwest Ohio, USA, are summarized. These changes involve canopy composition and structure, turnover in snags, and development of vegetation in treefall gaps. Stand basal area and canopy density have changed little in 32 years. However, beech has decreased in canopy importance (49% to 32%) while sugar maple has increased (32% to 47%). Annual mortality was about 1.3% throughout the study period. Mortality rates increased with stem size, but the fraction of larger stems increased due to ingrowth from smaller size classes. Beech was represented by more very large stems than small canopy stems: over time, death of those larger stems with inadequate replacement has caused the decrease in beech importance. Sugar maple was represented by more small canopy stems whose growth has increased its importance. The changes in beech and sugar maple relative importance are hypothesized to be due to forest fragmentation mostly from the early 1800s with some possible additional effects associated with the formation of the state park. Snag densities (12-16 snags/ha) and formation rates (1-3 snags.ha(-1).yr(-1)) remained consistent. The treefall gaps previously studied are closing, with a few, large stems remaining. Death of gap border trees occurs consistently enough to favor species able to combine growth in gaps and survival in the understory.

  15. Increased seedling establishment via enemy release at the upper elevational range limit of sugar maple.

    Science.gov (United States)

    Urli, Morgane; Brown, Carissa D; Narváez Perez, Rosela; Chagnon, Pierre-Luc; Vellend, Mark

    2016-11-01

    The enemy release hypothesis is frequently invoked to explain invasion by nonnative species, but studies focusing on the influence of enemies on natural plant range expansion due to climate change remain scarce. We combined multiple approaches to study the influence of plant-enemy interactions on the upper elevational range limit of sugar maple (Acer saccharum) in southeastern Québec, Canada, where a previous study had demonstrated intense seed predation just beyond the range limit. Consistent with the hypothesis of release from natural enemies at the range limit, data from both natural patterns of regeneration and from seed and seedling transplant experiments showed higher seedling densities at the range edge than in the core of the species' distribution. A growth chamber experiment manipulating soil origin and temperature indicated that this so-called "happy edge" was not likely caused by temperature (i.e., the possibility that climate warming has made high elevation temperatures optimal for sugar maple) or by abiotic soil factors that vary along the elevational gradient. Finally, an insect-herbivore-exclusion experiment showed that insect herbivory was a major cause of seedling mortality in the core of sugar maple's distribution, whereas seedlings transplanted at or beyond the range edge experienced minimal herbivory (i.e., enemy release). Insect herbivory did not completely explain the high levels of seedling mortality in the core of the species' distribution, suggesting that seedlings at or beyond the range edge may also experience release from pathogens. In sum, while some effects of enemies are magnified beyond range edges (e.g., seed predation), others are dampened at and beyond the range edge (e.g., insect herbivory), such that understanding the net outcome of different biotic interactions within, at and beyond the edge of distribution is critical to predicting species' responses to global change. © 2016 by the Ecological Society of America.

  16. Norway maple displays greater seasonal growth and phenotypic plasticity to light than native sugar maple.

    Science.gov (United States)

    Paquette, Alain; Fontaine, Bastien; Berninger, Frank; Dubois, Karine; Lechowicz, Martin J; Messier, Christian; Posada, Juan M; Valladares, Fernando; Brisson, Jacques

    2012-11-01

    Norway maple (Acer platanoides L), which is among the most invasive tree species in forests of eastern North America, is associated with reduced regeneration of the related native species, sugar maple (Acer saccharum Marsh) and other native flora. To identify traits conferring an advantage to Norway maple, we grew both species through an entire growing season under simulated light regimes mimicking a closed forest understorey vs. a canopy disturbance (gap). Dynamic shade-houses providing a succession of high-intensity direct-light events between longer periods of low, diffuse light were used to simulate the light regimes. We assessed seedling height growth three times in the season, as well as stem diameter, maximum photosynthetic capacity, biomass allocation above- and below-ground, seasonal phenology and phenotypic plasticity. Given the north European provenance of Norway maple, we also investigated the possibility that its growth in North America might be increased by delayed fall senescence. We found that Norway maple had significantly greater photosynthetic capacity in both light regimes and grew larger in stem diameter than sugar maple. The differences in below- and above-ground biomass, stem diameter, height and maximum photosynthesis were especially important in the simulated gap where Norway maple continued extension growth during the late fall. In the gap regime sugar maple had a significantly higher root : shoot ratio that could confer an advantage in the deepest shade of closed understorey and under water stress or browsing pressure. Norway maple is especially invasive following canopy disturbance where the opposite (low root : shoot ratio) could confer a competitive advantage. Considering the effects of global change in extending the potential growing season, we anticipate that the invasiveness of Norway maple will increase in the future.

  17. The Role of Forest Tent Caterpillar Defoliations and Partial Harvest in the Decline and Death of Sugar Maple

    Science.gov (United States)

    Hartmann, Henrik; Messier, Christian

    2008-01-01

    Background and Aims Natural and anthropogenic disturbances can act as stresses on tree vigour. According to Manion's conceptual model of tree disease, the initial vigour of trees decreases as a result of predisposing factors that render these trees more vulnerable to severe inciting stresses, stresses that can then cause final vigour decline and subsequent tree death. This tree disease model was tested in sugar maple (Acer saccharum) by assessing the roles of natural and anthropogenic disturbances in tree decline and death. Methods Radial growth data from 377 sugar maple trees that had undergone both defoliations by insects and partial harvest were used to estimate longitudinal survival probabilities as a proxy for tree vigour. Radial growth rates and survival probabilities were compared among trees subjected to different levels of above- and below-ground disturbances, between periods of defoliation and harvest, and between live and dead trees. Key Results Manion's tree disease model correctly accounts for vigour decline and tree death in sugar maple; tree growth and vigour were negatively affected by a first defoliation, predisposing these trees to death later during the study period due to a second insect outbreak that initiated a final vigour decline. This decline was accelerated by the partial harvest disturbance in 1993. Even the most severe anthropogenic disturbances from partial harvest did not cause, unlike insect defoliation, any growth or vigour declines in live sugar maple. Conclusions Natural disturbances acted as predisposing and inciting stresses in tree sugar maple decline and death. Anthropogenic disturbances from a partial harvest at worst accelerated a decline in trees that were already weakened by predisposing and inciting stresses (i.e. repeated insect defoliations). Favourable climatic conditions just before and after the partial harvest may have alleviated possible negative effects on growth resulting from harvesting. PMID:18660493

  18. Soil quality indicators in a rhodic kandiudult under different uses in northern Parana, Brazil

    Directory of Open Access Journals (Sweden)

    Biana Harumi Kuwano

    2014-02-01

    Full Text Available Sustainable use of soil, maintaining or improving its quality, is one of the goals of diversification in farmlands. From this point of view, bioindicators associated with C, N and P cycling can be used in assessments of land-use effects on soil quality. The aim of this study was to investigate chemical, microbiological and biochemical properties of soil associated with C, N and P under different land uses in a farm property with diversified activity in northern Parana, Brazil. Seven areas under different land uses were assessed: fragment of native Atlantic Forest; growing of peach-palm (Bactrys gasipaes; sugarcane ratoon (Saccharum officinarum recently harvested, under renewal; growing of coffee (Coffea arabica intercropped with tree species; recent reforestation (1 year with native tree species, previously under annual crops; annual crops under no-tillage, rye (Cecale cereale; secondary forest, regenerated after abandonment (for 20 years of an avocado (Persea americana orchard. The soil under coffee, recent reforestation and secondary forest showed higher concentrations of organic carbon, but microbial biomass and enzyme activities were higher in soils under native forest and secondary forest, which also showed the lowest metabolic coefficient, followed by the peach-palm area. The lowest content of water-dispersible clay was found in the soil under native forest, differing from soils under sugarcane and secondary forest. Soil cover and soil use affected total organic C contents and soil enzyme and microbial activities, such that more intensive agricultural uses had deeper impacts on the indicators assessed. Calculation of the mean soil quality index showed that the secondary forest was closest to the fragment of native forest, followed by the peach-palm area, coffee-growing area, annual crop area, the area of recent reforestation and the sugarcane ratoon area.

  19. Modeled effects of soil acidification on long-term ecological and economic outcomes for managed forests in the Adirondack region (USA)

    Science.gov (United States)

    Caputo, Jesse PhD.; Beier, Colin M.; Sullivan, Timothy J.; Lawrence, Gregory B.

    2016-01-01

    Sugar maple (Acer saccharum) is among the most ecologically and economically important tree species in North America, and its growth and regeneration is often the focus of silvicultural practices in northern hardwood forests. A key stressor for sugar maple (SM) is acid rain, which depletes base cations from poorly-buffered forest soils and has been associated with much lower SM vigor, growth, and recruitment. However, the potential interactions between forest management and soil acidification – and their implications for the sustainability of SM and its economic and cultural benefits – have not been investigated. In this study, we simulated the development of 50 extant SM stands in the western Adirondack region of NY (USA) for 100 years under different soil chemical conditions and silvicultural prescriptions. We found that interactions between management prescription and soil base saturation will strongly shape the ability to maintain SM in managed forests. Below 12% base saturation, SM did not regenerate sufficiently after harvest and was replaced mainly by red maple (Acer rubrum) and American beech (Fagus grandifolia). Loss of SM on acid-impaired sites was predicted regardless of whether the shelterwood or diameter-limit prescriptions were used. On soils with sufficient base saturation, models predicted that SM will regenerate after harvest and be sustained for future rotations. We then estimated how these different post-harvest outcomes, mediated by acid impairment of forest soils, would affect the potential monetary value of ecosystem services provided by SM forests. Model simulations indicated that a management strategy focused on syrup production – although not feasible across the vast areas where acid impairment has occurred – may generate the greatest economic return. Although pollution from acid rain is declining, its long-term legacy in forest soils will shape future options for sustainable forestry and ecosystem stewardship in the northern

  20. An Eco-hydrologic Assessment of Small Experimental Catchments with Various Land Uses within the Panama Canal Watershed: Agua Salud Project

    Science.gov (United States)

    Crouch, T. D.; Ogden, F. L.; Stallard, R. F.; Smithsonian Tropical Research Institute, Panama Canal Watershed Experiment, Agua Salud Project

    2010-12-01

    Hydrological processes in the humid tropics are poorly understood and an important topic when it comes to water management in the seasonal tropics. The Smithsonian Tropical Research Institute, Panama Canal Watershed Experiment, Agua Salud Project, seeks to understand these processes and quantify the long-term effects of different land cover and uses across the Panama Canal Watershed. One of the project’s main objectives is to understand how reforestation effects seasonal stream flows. To meet this objective, a baseline characterization of hydrology on the small catchment scale is being assessed across different land uses typical in rural Panama. The small experimental catchments are found within Panama’s protected Soberania National Park and the adjacent headwaters of the Agua Salud and Mendoza Rivers, all of which are part of the greater Panama Canal Watershed. The land uses being monitored include a variety of control catchments as well as treated pasture sites. The catchments used for this study include a mature old regrowth forest, a 50% deforested or mosaic regrowth site, an active pasture and a monoculture invasive grass site (saccharum spontaneum) as experimental controls and two treated catchments that were recently abandoned pastures converted to teak and native species timber plantations. Installed instrumentation includes a network of rain gauges, v-notched weirs, atmometers, an eddy covariance system and an assortment of meteorological and automated geochemical sampling systems. Spatial, rainfall, runoff and ET data across these six geologically and topographically similar catchments are available from 2009 and 2010. Classic water balance and paired catchment techniques were used to compare the catchments on an annual, seasonal, and event basis. This study sets the stage for hydrologic modeling and for better understanding the effects of vegetation and land-use history on rainfall-runoff processes for the Agua Salud Project and Panama Canal