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Sample records for saibo baiyo shisutemu

  1. Research and development of basic technologies for next-generation industries. Evaluation of 2nd phase research and development of technologies of mass culture of cells; Jisedai sangyo kiban gijutsu kenkyu kaihatsu saibo tairyo baiyo gijutsu. Dainiki kenkyu kaihatsu hyoka

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1987-03-30

    The project aims to establish basic technologies for the mass culture of cells which will enable a large, stable supply of fine chemical products whose industrial production the conventional technique of synthesis has failed to realize. In the 1st phase, cell stains were selected, a serumless medium was developed, basic culturing conditions were established, and substances production rates were improved, and the 2nd phase stands succeeding these 1st phase achievements. Toward the ultimate goal of establishing basic technologies for serumless high-density cultures for industrial use and technologies for isolating and refining useful substances, a small-scale optimum culture technique and a serumless culture technique are developed. Serumless cultures are developed for strains of man's lymphoid cells, cells deriving from bone marrow, epithelial cells, and hybridomas of man and mouse, all these sampled during the 1st phase endeavors. Success is attained in growing each cell strain on a scale of 1-10L to increase to 10{sup 7} cells/ml or more in population density. It is found that each cell strain produces useful substances, such as man's monoclonal antibodies and elements involved in the multiplication or differentiation of new cells. (NEDO)

  2. Research and development of basic technologies for next-generation industries. Evaluation of 2nd phase research and development of technologies of mass culture of cells; Jisedai sangyo kiban gijutsu kenkyu kaihatsu saibo tairyo baiyo gijutsu. Dainiki kenkyu kaihatsu hyoka

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1987-03-30

    The project aims to establish basic technologies for the mass culture of cells which will enable a large, stable supply of fine chemical products whose industrial production the conventional technique of synthesis has failed to realize. In the 1st phase, cell stains were selected, a serumless medium was developed, basic culturing conditions were established, and substances production rates were improved, and the 2nd phase stands succeeding these 1st phase achievements. Toward the ultimate goal of establishing basic technologies for serumless high-density cultures for industrial use and technologies for isolating and refining useful substances, a small-scale optimum culture technique and a serumless culture technique are developed. Serumless cultures are developed for strains of man's lymphoid cells, cells deriving from bone marrow, epithelial cells, and hybridomas of man and mouse, all these sampled during the 1st phase endeavors. Success is attained in growing each cell strain on a scale of 1-10L to increase to 10{sup 7} cells/ml or more in population density. It is found that each cell strain produces useful substances, such as man's monoclonal antibodies and elements involved in the multiplication or differentiation of new cells. (NEDO)

  3. Research, development, and evaluation of basic technologies for next-generation industries. Technologies of mass culture of cells; Jisedai sangyo kiban gijutsu kenkyu kaihatsu hyoka. Saibo tairyo baiyo gijutsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1990-03-01

    The project aims to establish basic technologies for the mass culture of cells by utilizing the features of animal cells, which will enable a large, stable supply of fine chemicals such as highly functional physiologically active proteins whose industrial production the conventional technique of synthesis fails to realize. For this purpose, efforts are exerted to select cells which are capable of producing target substances, to breed highly productive strains, to develop a serumless medium that satisfies demands for an all-purpose feature and heat sterilization, and to develop technologies of high-density culture and effective refining, etc. Some DNA (deoxyribonucleic acid) recombination technologies are also resorted to. As the result, a high-density culture of 2 times 10{sup 8} cells/ml has become available. Production is now feasible of various differentiation-inducing elements such as monoclonal antibodies with anti-HB virus activity, DIF, etc. Furthermore, cooperation with the Tsukuba University leads to the discovery of a novel vasopressor peptide (endothelin). The heat sterilization accepting serumless medium, which is put to practical use as the first of the kind in the world under this research and development project, contributes to the advancement of basic studies about cell biology. (NEDO)

  4. Fermentation characteristics in conversion of organic acids obtained by oxidation of low-rank coals to poly({beta}-hydroxybutyrate) using A. eutrophus cells with some analysis on metabolic flux distribution; Kattan no ekisosanka de erareru yukisan wo suiso saikin wo riyoshite pori {beta}-hidorokishi rakusan (PHB) ni henkansaseru tameno baiyo kogakuteki kento to taisha ryusoku bunpu shisutemu kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Tsujimoto, Shoko.; Shin, Huidong.; Shimizu, Kazuyuki. [Kyushu Institute of Technology, Fukuoka (Japan). Department of Biochemical engineering and science; Mae, Kazuhiro.; Miura, Koichi. [Kyoto University, Kyoto (Japan). Department of Chemical Engineering

    1999-03-10

    Fermentation characteristics are investigated for the conversion of glycolate, acetate, formate, and malonate obtained by the oxidation of low-rank coals to poly ({beta}-hydrox butyrate) (PHB) using A. eutrophus cells. Based on the cultivation experiments using one of the organic acids as a sole carbon source, it is found that acetate is the most effectively converted to PHB. When mixed organic acids are used, formate is preferentially consumed, followed by acetate, and finally glycolate. Although malate can not be utilized, it is implied that it might change the pathway flux distributions based on the metabolic flux analysis. Namely, it shows competitive inhibition to succinate dehydrogenase so that its addition during fermentation results in flux reduction from succinate to maleic acid as well as glyoxylate flux and gluconeogenesis flux. It is also found that NADPH generated from isocitrate is preferentially utilized for the reaction from {alpha}-ketoglutarate to glutamate when NH{sub 3} concentration is high, while it is eventually used for the PHB production from acetoacetyl CoA as NH{sub 3} concentration decreases. (author)

  5. Achievement report on commissioned research and development of basic technologies for the next generation industries in fiscal 1981. Research and development of a voluminous cell incubation technology; 1981 nendo jisedai sangyo kiban gijutsu itaku kenkyu kaihatsu seika hokokusho. Saibo tairyo baiyo gijutsu no kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1982-03-01

    Achievements were compiled on the research and development in fiscal 1981 on the 'voluminous cell incubation technology', which has been performed according to the 'institution for research and development of basic technologies for the next generation industries'. In the research of a method for producing industrial materials using cells originated from lymph-based trunk cells, researches were performed on selection and breeding of cells, development of serum-free media, and establishment of the optimal culture method with regard to the method for producing industrial materials using stocks producing virus induced interferon. Regarding the method for producing industrial materials using idio-interferon producing mutable stocks, research and development was made on selection and breeding of superior cell stocks and serum-free media. With regard to the method for producing industrial materials using marrow originated cells, such researches were carried out as technological investigations, collection of cells, establishment of the proliferating condition for myeloid leukemia cells, discussions on the assay method for differentiation inducing factors, and search for cells producing useful substances. Other activities include research of the method for producing industrial materials using cells originated from epithelium cells, information exchange on voluminous cell incubation technologies, and investigations on literatures and patents. (NEDO)

  6. Achievement report on commissioned research and development of basic technologies for the next generation industries in fiscal 1981. Research and development of a voluminous cell incubation technology; 1981 nendo jisedai sangyo kiban gijutsu itaku kenkyu kaihatsu seika hokokusho. Saibo tairyo baiyo gijutsu no kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1982-03-01

    Achievements were compiled on the research and development in fiscal 1981 on the 'voluminous cell incubation technology', which has been performed according to the 'institution for research and development of basic technologies for the next generation industries'. In the research of a method for producing industrial materials using cells originated from lymph-based trunk cells, researches were performed on selection and breeding of cells, development of serum-free media, and establishment of the optimal culture method with regard to the method for producing industrial materials using stocks producing virus induced interferon. Regarding the method for producing industrial materials using idio-interferon producing mutable stocks, research and development was made on selection and breeding of superior cell stocks and serum-free media. With regard to the method for producing industrial materials using marrow originated cells, such researches were carried out as technological investigations, collection of cells, establishment of the proliferating condition for myeloid leukemia cells, discussions on the assay method for differentiation inducing factors, and search for cells producing useful substances. Other activities include research of the method for producing industrial materials using cells originated from epithelium cells, information exchange on voluminous cell incubation technologies, and investigations on literatures and patents. (NEDO)

  7. Achievement report for fiscal 1987 on the entrusted research and development of basic technologies for next-generation industries. Research and development of technologies of mass culture of cells; 1987 nendo jisedai sangyo kiban gijutsu kenkyu kaihatsu seika hokokusho. Saibo tairyo baiyo gijutsu no kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1988-03-01

    The aim is to add value to the chemical industry by establishing technologies for realizing a large and stable supply of fine chemical products whose industrial production is difficult when conventional techniques are applied, the products including various useful substances yielded by animal cells including those of man. To fulfill the aim, efforts are exerted to complete basic technologies involving the mass culture of cells through conducting researches into animal biofunctions. This report covers the endeavors made in fiscal 1987 under the above-named project created in fiscal 1981. All the subject matters are for the development of substance production methods for industrial use, and include the studies of use of optimal cultivation methods mainly for serumless cultures and cultivation engineering, studies centering on the high-density cultivation of cells and serumless cultures, studies using suspended and quasi-suspended cell lines, studies using cells deriving from bone marrow, and studies using epithelial cells. Furthermore, the results are outlined of the comprehensive researches conducted into technologies of mass cultivation of cells. (NEDO)

  8. Culture engineering examination and metabolism flux distribution system analysis for madding to convert into poly {beta}- hydroxybutyric acid (PHB) using the hydrogen bacteria of organic acid got in liquid-phase oxidation of lignite; Kattan no ekiso sanka de erareru yukisan wo suiso saikin wo riyoshite pori {beta}-hidorokishi rakusan(PHB) ni henkan saseru tameno baiyo kogakuteki kento to taisha ryusoku bunpu shisutemu kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Tsujimoto, Kinko; Seki, Suito; Shimizu, Kazuyuki; Mae, Kazuhiro; Miura, Koichi

    1999-04-05

    The culture engineering examination for madding to convert into poly {beta} - hydroxy Wisteria (PHB) which glycolic acid. Acetic acid, ant acid, malonic acid got in liquid-phase oxidation of lignite are raw material of biodegradable plastic using hydrogen bacteria Alcaligenes eutrophus was carried out. It was proven that acetic acid was the most efficiently converted into the PHB as a result of cultivating these organic acid as a single carbon source. And, it was utilized to the bacterial cell at the order of ant acid, acetic acid, glycolic acid, when it was cultivated in mixing organic acid, and it was proven to convert into the PHB. Though the malonic acid was not utilized for the bacterial cell breeding, it was indicated that as the result which analyzed metabolism flow distribution by calculating using the culture data, the succinate dehydrogenase of the tricarboxylic acid (TCA) circuit received competitive inhibition, when this is added in culture middle point, and that the flux of griot lysyl acid route and gluconeogenesis route lowers. And, it was proven that it was utilized in the route which comes to the PHB synthesis from acetoacetyl CoA with the lowering of the ammonia concentration on NADPH produced from the isocitric acid, though it was prior consumed to the glutamic acid of tricarboxylic acid cycle in the route, if ammonia concentration is high. (translated by NEDO)

  9. Distribution system of prepared foods; Chorizumi shokuhin no ryutsu shisutemu

    Energy Technology Data Exchange (ETDEWEB)

    Kaneko, J.

    1999-09-05

    Chains such as supermarket, convenience store, and delicatessen store are rapidly expanding business. Only of thing and value thing which is only novel enjoyably also consumer needs these cooked family restaurant food service industry menu be. The following would be expected: Goods and menu of surely coming to the hand in result of being safer and result of dealing with the diversification of the flavor, the case in which it is necessary. Recently, it came to the distribution reform of the cooked food remarkably and drastically reducing the distribution cost as the result. (NEDO)

  10. Ceramics adsorbing virus and cells. Uirusu, saibo bunri ceramics

    Energy Technology Data Exchange (ETDEWEB)

    Hiraide, T. (Asahi Optical Co. Ltd., Tokyo (Japan))

    1993-07-01

    It has been reported that hydroxyapatite (HA), which is the main inorganic component of teeth and bones of homo sapiens and used for biomaterials such as artificial tooth roots, adsorbs viruses such as influenza viruses. In this article, the history of development up to now of HA and its adsorption mechanism of protein, virus, etc., are introduced. HA was applied for chromatography in 1956 becoming one of the separating and refining methods of protein and nucleic acid, then after the development of spherical porous HA, it has become applied for high speed liquid chromatography (HPLC). Also by means of a column filled with HA granules, T-cells have been able to be purified in a short time from lymphocyte which was separated from the blood of homo sapiens. Recently it has also been reported that HA granules can adsorb influenza viruses, Japanese encephalitis viruses, polio viruses and hepatitis B viruses, and a cold-preventative mask based upon this report is now on sale. 11 refs., 7 figs.

  11. Function of mammalian genes regulation cellular growth; Saibo zoshoku wo seigyosuru dobutsu saibo idenshi no kino kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, K. [Nagoya University, Nagoya (Japan)

    1995-12-15

    Intracellular signaling from receptor tyrosine kindles in mammalian cells results in activation of a signal cascade that includes the guanine nucleotide binding protein Ras and the protein kinases Raf, MEK [Mitogen activated protein kindle (MAPK) or Extracellular signal regulated kinase (ERK) kinase] and MAPK. MAPK activation that is dependent on the coupling of Ras and Raf was reconstituted in yeast. Yeast genes were isolated that, when overexpressed, enhanced the function of Raf. One of them is identical to BMH 1, which encodes a protein similar to members of the mammalian 14-3-3 family. Bacterially synthesized mammalian 14-3-3 protein stimulated the activity of Raf prepared from yeast cells expressing c-Raf-1. Thus, the 14-3-3 protein may participate in or be required for activation of Raf. 9 refs., 2 figs.

  12. Trend of development of dispersion energy machine. Solar power generation system; Taiyoko hatsuden shisutemu

    Energy Technology Data Exchange (ETDEWEB)

    Oda, T. [New Energy and Industrial Technology Development Organization, Tokyo (Japan)

    2000-04-01

    Technology development of a solar power generation system is advanced in Japan taking the New Sunshine Subject (NSS) of the Agency of Industrial Science and Technology as the center. According to the initial stage plan (in fiscal 1993 to 2000) of the NSS, a general goal is to establish the technique which supplies electric power at the power generating cost (the production base cost) being equal to the electricity fees (20 to 30 yen/kWh) for normal families in the latest year. At present, introduction of the solar power generation system results in the reduction of about 1 million yen per kW. It is expected that 5 hundred thousand yen per kW is reduced by the present technology with a combination of cost reduction of solar battery modules, cost reduction of relevant equipment such as inverters, and rationalization of installation fees. It is necessary to realize 3 hundred thousand yen per kW for realizing the price having competition in the power supply market. In this paper, popularization prediction and popularization promotion countermeasures of the solar power generation system are concretely explained. (NEDO)

  13. Leading research on artificial techniques controlling cellular function; Saibo zoshoku seigyo gijutsu no sendo kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    Advanced research and its applicability were surveyed to apply the advanced functional cells to industry. The basic target was set to develop, produce, control and utilize the functional cells, such as intelligent materials and self-regulation bioreactors. The regulation factors regarding apotosis, which is a process of cell suicide programmed within the cell itself of multicellular organisms, cell cycle and aging/ageless were investigated. Furthermore, the function of regulatory factors was investigated at the protein level. Injection of factors regulating cellular function and tissue engineering required for the regulation of cell proliferation were investigated. Tissue engineering is considered to be the intracellular regulation by gene transduction and the extracellular regulation by culture methods, such as coculture. Analysis methods for cell proliferation and function of living cells were investigated using the probes recognizing molecular structure. Novel biomaterials, artificial organ systems, cellular therapy and useful materials were investigated for utilizing the regulation techniques of cell proliferation. 425 refs., 85 figs., 9 tabs.

  14. Leading research on cell proliferation regulation technology; Saibo zoshoku seigyo gijutsu no sendo kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    For developing intelligent material, animal test alternative model, bio-cell analysis equipment, self-controlling bio-reactor and medical material, development of functional cells was studied by cell proliferation regulation technology. In fiscal 1996, the expression analysis and separation technology of specific gene for cell proliferation, and the intracellular regulation technology were surveyed from the viewpoint of intracellular regulation. The cell proliferation regulation technology by specific regulating material of cells, extracellular matrix, coculture system and embryonic cell was surveyed from the viewpoint of extracellular regulation. In addition, based on these survey results, new cell culture/analysis technology, new bio-material, artificial organ system, energy saving bio-reactor, environment purification microorganism, and animal test alternative model were surveyed as applications to industrial basic technologies from a long-term viewpoint. The approach to cell proliferation regulation requires preparation of a concrete proliferation regulation technology system of cells, and concrete application targets. 268 refs., 43 figs., 4 tabs.

  15. Fresh Vegetables in the food service Industry its purchasing system; Gaishoku sangyo ni okeru seisen seikabutsu no chotatsu shisutemu

    Energy Technology Data Exchange (ETDEWEB)

    Oda, K. [Food Service Industry Survey and Research Center, Tokyo (Japan)

    1999-09-05

    The environment which surrounds production, distribution, consumption of vegetables changes. A demand for the business a household economy demand of vegetables stagnates by the increase of eating out and lunches demand in the food consumption as a long-term tendency, and the tendency as vigorousness continues. In addition, a demand for domestic organic and special cultivation vegetables from the increase of the healthy and safe orientation of the consumer heightens. In this paper, what kind of new movement occurs in environmental change which surrounds these vegetables is arranged. (NEDO)

  16. Application of cytotoxicity test for toxic micropollutants. Saibo dokusei shiken ni yoru yugai kagaku busshitsu osen no hyoka

    Energy Technology Data Exchange (ETDEWEB)

    Utsumi, H; Hamada, A [Showa University, Tokyo (Japan). School of Pharmaceutical Science; Ono, Y [National Institute of Hygienic Sciences, Tokyo (Japan)

    1992-10-01

    Considerations were given from a viewpoint of assessing toxicity to human bodies on methods of assessing water pollutants and organisms, and applicability of cytotoxity test using cultured cells to water quality assessment. Biological assessment systems used for water environment may use tests using multicellular organisms, cells, or organelles in cells. The organism assessment method is intended mainly for assessing ecological effects, and a suitable method must be selected upon extrapolating it to human bodies. A toxicity parameter used most frequently in a cytotoxity test is the cell revival rate, and life and death are determined from liberation of enzymes in cells, or with color rejection tests and incorporation tests. There are a number of test specimens of raw tap water and its chlorine treatment condensate that show no mutagenicity but cytotoxity. Efficiencies of removal by means of mild chlorine treatment, fast filtration, and activated carbon adsorption vary greatly with cytotoxity and mutagenicity. Introducing the cytotoxity test is expected of further contributing to improving safety in water quality. 24 refs., 1 fig., 7 tabs.

  17. Monitoring on-line system for the lactic fermentation measurement using the integration enzyme sensor; Shusekika koso sensa wo mochiita nyusan hakko keisokuyo onrain monitringu shisutemu

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Masayasu; Kumagaya, Tsuyoshi; Nakajima, Yoichi [Kyushu Institute of Technology, Fukuoka (Japan)

    1999-04-05

    The monitoring on-line system for the lactic fermentation measurement in which the simultaneous measurement of the substrate. Generation was possible was constructed without consuming the culture medium by using soliciting small enzyme sensor and flow injection analysis system integrate. There was the linearity that anyway was also range of concentration of 70mM or less and that it is good on the calibration curve of minute glucose, lactose, and lactic acid sensor. It became clear that it proved that all range of concentration of the substrate of these three which combining with the micro diary system, breaks in the lactic fermentation measurement with the necessity can be measured and not observe the interference by medium components, etc. either. Constructed monitoring on-line system is Lactobacillus delbrueckii and, it was applied to the lactic fermentation process of Lactobacillus lactis. Through the fermentation process for 24 hours, simultaneous measurement of glucose (or lactose) and lactic acid is possible. The measured value agreed well with the result of colorimetric method using the enzyme. (translated by NEDO)

  18. On-line monitoring system of lactic acid fermentation by using integrated enzyme sons ors; Shusekika koso sensa wo mochiita nyusan hakko keisokuyo onrain monitaringu shisutemu

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Masayasu; Kumagi, Takeshi; Nakashima, Yuuichi [Kyushu University, Fukuoka (Japan). Dept. of Biochemical Engineering and Science

    1999-03-10

    An on-line monitoring system for lactic acid fermentation is developed by using integrated micro enzyme sensors, a flow injection analysis system, and a micro dialysis system. The calibration curves of micro glucose, lactose and lactate sensors show good linearity in the concentration range below 70 mM. By combination with the micro dialysis system, the enzyme sensors can measure the whole concentration range of lactic acid fermentation, and interference by the medium can not be observed. The on-line sensor system is then applied to lactic acid fermentation of Lactobacillus delbrueckii. The sensor system can monitor the glucose and lactate concentrations simultaneously during 24-h fermentation, and the measurements show good agreement with those of the conventional colorimetric method. The sensor system can also be applied to on-line monitoring of lactose and lactate during Lactobacillus lactis fermentation. (author)

  19. Output characteristics of 40 kW photovoltaic power generation system in ICT; Ibaraki kosen ni okeru 40 kW taiyoko hatsuden shisutemu no shutsuryoku tokusei

    Energy Technology Data Exchange (ETDEWEB)

    Sekiguchi, N. [Ibaraki National College of Tech., Ibaraki (Japan); Tani, T. [Science University of Tokyo, Tokyo (Japan)

    1999-11-25

    The photovoltaic power generation system consists of photovoltaic array and power conditioner of the utility connected system. The photovoltaic array parallelly constitutes 18 serial 30 of the modules of 540 sheets, and there is the generating capacity of largest 40 kW. The power conditioner uses 10 kW four units, and it is tracking with function of the maximum output point. This report examined the unconformable rate of photovoltaic array maximum output operating voltage, current and power in simulation and power conditioner input. (author)

  20. Independent power source hybrid system - recent examples mainly of mountain huts.; Dokuritsu dengengata no haiburiddo shisutemu -saikin no yamagoya deno jirei wo chushin ni.

    Energy Technology Data Exchange (ETDEWEB)

    Mori, T. [Kanagawa Inst. of Tech., Kanagawa (Japan)

    2000-09-30

    History of the independent power source hybrid systems used at such as mountain huts were outlined, and recent application examples of the hybrid systems were explained. At Natsuzawa mineral spring in Nagano pref., 7 kW of hybrid power generator system composed of solar cell and wind power generator, as well as 400 W of small hydraulic power generator are working supplying electric power for the private sewerage system, and the system without diesel generator is being tested. At Senjogahara refuge hut in South Alps, a hybrid power generation system composed of 10.7 kW of solar cell and 6.4 kW of wind power generator was installed, and is working. In mountainous area, there exist critical factors such as weather condition and difficulty in carrying equipment, accordingly, cost reduction and sizing down of relevant apparatus such as batteries and inverters are expected. (NEDO)

  1. Effect of applied synthetic auxin on root growth in plantlet propagation by cuttage and tissue culture; Sashiki to soshiki baiyo ni okeru gosei auxin rui no shiyo koka

    Energy Technology Data Exchange (ETDEWEB)

    Shoji, K; Yoshihara, T [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1996-12-01

    The effect of synthetic plant hormone 4-C1-IAA and TFIBA on root growth in plantlet propagation was clarified by the cuttage and the issue culture of strawberry seedling production. A periwinkle, vine, and azalea are the effect of 4-C1-IAA on root growth, and a promotion effect was recognized for rooting and root elongation. The concentration of 4-C1-IAA in which the growth promotion effect of a root most appears varies depending on the species of a plant. The concentration of a periwinkle was 20 ppm, and that of an azalea was 2000 ppm. The growth promotion effect of a root in 4-C1-IAA and TFIBA was compared with IBA for an azalea. The result showed that 4-C1-IAA is the same in the effect as IBA and that TFIBA is higher than for IBA. The growth of a vine`s terminal bud was promoted by the effect of TFIBA on root growth, and the callus occurring when IBA was treated was not formed. The rooting of a strawberry was promoted by the effect of TFIBA on root growth. The combined use of TFIBA and BA promotes the growth of a side bud and forms a multi-bud plant. However, rooting was inhibited. The callus caused by the effect of BA on root growth could be suppressed through the combined use with TFIBA. 6 refs., 7 figs., 2 tabs.

  2. Fiscal 1999 leading research report. Research on 3-D cell tissue module technology; 1999 nendo sanjigen saibo soshiki module kogaku chosa kenkyu hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For forming cell tissues alternative to bio-tissues, this research targets the technology for forming bio-tissue modules by cultivating 3-D cell tissues from various kinds of cells. In fiscal 1999, research was made on various issues of element technologies necessary for forming module structure of bio-tissues, and study was also made on the application of 3-D cell tissue module engineering to industrial fields. Survey was made on element technologies and solutions supporting such engineering, and in addition, on progressive conditions of cell cultivation techniques, and concrete techniques for expressing cell functions. Research was made on a cell behavior under physicochemical stimulus environment to study the optimum environment for cell multiplication and function expression. Further research was made on state analysis of cells, in particular, fast precise measurement techniques of activation or malignant of cells and secretion of toxic substances by physical/optical analytical evaluation techniques such as photo-CT and spectroscopic analysis, and biochemical analysis techniques using bio-sensors. Study was also made on successful development and application cases of practical bio-artificial organs in western countries. (NEDO)

  3. Study of the influence of microgravity on the biological cells and molecular level; Seitai saibo bunshi level ni okeru bisho juryoku no eikyo ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    The shape of osteoblast, gene appearance, gene of rice blast, cellular fusion of plants, gravity acceptance mechanism of unicellular organisms, and physiological and immunity functions of mice were investigated under the microgravity condition. The influence of gravity on the vital reaction and the influence of microgravity on the crystallization of vital substances were also investigated. For the observation of osteoblast, the fluorescence dye reacted with Ca was well taken in the cells. The microgravity affected the stability of rice blast, but hardly affected the protoplast culture of mushroom. The reaction of ciliate against the gravity related to the specific gravity difference between cells and outer liquid. The level of adrenaline in blood of mice increased during the drop. The moving speed of trigger waves of chemical parallel slit formed at the BZ reaction under the microgravity became 60% to 80% of that on the ground. In the case of crystallization at the deposition agent concentration of 1% to 4%, the turbidity showing the degree of crystallization changed complicatedly. Nine processes of crystal growth were recognized. 21 refs., 55 figs., 1 tab.

  4. Changes of inflammatory cells in rat lungs exposed to diesel emissions; Diesel haiki bakuro ni yoru rat hai no ensho saibo no henka

    Energy Technology Data Exchange (ETDEWEB)

    Kato, A. [Japan Automobile Research Institute Inc., Tsukuba (Japan); Kagawa, J. [Tokyo Women`s Medical College, Tokyo (Japan)

    1998-05-01

    Study was made on the effect of exposure to diesel emissions on inflammatory cells in a rat lungs. Four kinds of exposure gases with different contents of NO2 and particulate were prepared by diluting diesel emissions. Rats were exposed to diluted diesel emissions for 24 months, and inflammatory cells were detected morphologically in light microscopic and TEM specimens. As a result, particle-laden- alveolar macrophages increased dose- and time-dependently into the submucosa of intrapulmonary bronchioles, alveolar spaces and interstitume of alveolar walls, and bronchoassociated lymphatic tissues. Mast cells infiltrated into the interspaces of epithelial cells in airways. In the submucosa of the terminal bronchioles and the interstitume of alveolar walls, some sorts of inflammatory cells such as mast cells, plasma cells, neutrophils and lymphocytes infiltrated, and some cells showed cell-to-cell contacts. However, the airways were rarely injured by infiltration of inflammatory cells except for a fibrotic change. 2 refs., 2 figs., 2 tabs.

  5. Development of technology for plantlet propagation by tissue culture. (3). Strawberry callus growth and changes in pH in liquid medium; Soshiki baiyo ni yoru shubyo tairyo zoshoku gijutsu no kaihatsu. (3). Ekitai baiyo ni okeru ichigo callus no zoshoku to baichi no pH henka

    Energy Technology Data Exchange (ETDEWEB)

    Yoshihara, T; Hanyo, H [Central Research Inst. of Electric Power Industry, Tokyo (Japan)

    1991-02-01

    Development of strawberry cultivation plants is described as a part of night-time power utilization activities for power load levelling. Strawberry calluses (undifferent tissue mass) cultured in a liquid medium reach the fastest growth period in one week and the steady state in four weeks. The callus growth shows the maximum value at this time, which was 20 times as much of the seedling. The medium pH changed in a range from 4 to 7. If the initial pH is 4.0 or higher, no difference is created in the callus propagation in the steady state period, but at 3.0, no propagation whatsoever. The pH after the fastest growth period converged to a range from 6.0 to 7.0, with the exception of initial pH at 3.0. The medium pH decreased as a result of pre-culture heating sterilization, formation of iron phosphate due to light irradiation, and organic acid release during the initial growth phase. The pH increased because of difference in the speed of absorbing ammonium and nitric acid during the later growth phase. The growth efficiency of 20 times is about the same as other plants. Since the pH change is maintained within the range from 4 to 7, which causes no difference in in growth, there is no need of adjusting the pH within this range. 18 refs., 15 figs., 3 tabs.

  6. Research and development of a high-temperature superconducting flywheel energy storage system. Research and development of the New Sunshine Program; Furaihoiru denryoku chozo shisutemu kenkyu kaihatsu. Nyu sanshain keikaku ni motozuku kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawa, Y. [New Energy and Industrial Technology Development Organization, Tokyo (Japan)

    1999-11-25

    The project conducted by NEDO for developing a high-temperature superconducting flywheel energy storage system is introduced; the two test results of fundamental studies are described. One is the measurement of levitation force and rotation loss of superconducting magnetic bearings composed of oxide superconducting bulks and permanent magnet composite. Two types of superconducting magnetic bearings. axial and radial types, were fabricated and tested. The other test was the fabrication and testing of two functional models. A small-sized superconducting flywheel model of the 0.5 kWh class was fabricated and tested. A medium-sized rotating functional model of the 10 kWh class was fabricated as well. (author)

  7. Measurement method of moving vehicle in the magnetic levitation and propulsion system using high-Tc superconducting bulks; Koon chodendo barukutai wo riyoshita fujo suishin shisutemu no suishin tokusei keisoku shuho

    Energy Technology Data Exchange (ETDEWEB)

    Fuwa, Y.; Mizuma, T.

    1999-06-07

    The transportation system using simple magnetic levitation system got in the combination of high-temperature superconductivity bulk body and permanent magnet, which caught a quantum magnetic flux, is devised in great numbers, and the model equipment has also been manufactured. In the meantime, a grasp of levitation and guide characteristics is necessary for the case in which this levitation system is applied to the simple individual transportation system, and the measurement of the levitation characteristics is indispensable for the reason. In the conventional research, the measurement of the levitation characteristics has not been very much made. There was due to be no technique which measured kinetic characteristics of noncontacting surfacing and running travel body. In this study, measuring method for measuring these levitation characteristics was devised, and it was applied to actual measurement by the production of the model test equipment. Through this measurement, the purpose of this study is to show with that it is applicable for the real test equipment as this measuring method judges possibility of application to simple transportation system. (NEDO)

  8. Introduction effect of a load levelling system in an electric power system with a photovoltaic and wind system; Taiyoko/furyoku hatsuden wo donyu shita denryoku keito ni okeru fuka heijunka shisutemu no donyu koka

    Energy Technology Data Exchange (ETDEWEB)

    Kenmoku, Y.; Sakakibara, T. [Toyohashi University of Technology, Aichi (Japan); Nakagawa, S. [Maizuru College of Technology, Kyoto (Japan); Kawamoto, T. [Shizuoka University, Shizuoka (Japan)

    1998-12-05

    Introduction effect of load levelling system by a battery in an electric power system by a battery in an electric power system with a PV and wind system is investigated. Charge and discharge power of the battery are determined from a load curve and every hour data of PV and wind output. Annual cost of the power system is calculated from the generating power and the capacity of each source via the installed utility capacity and the capacity factor. It is found that (1) the battery system reduces the maximum demand and improves the load factor, (2) the cost effect of the battery system when introducing the PV system is higher than that when introducing the wind system. (author)

  9. Improvement of life and NO{sub x} emission of radiant tube heating system by elastic-plastic creep analysis; Dansosei kuripu kaiseki ni yoru hosha dennetsukan kanetsu shisutemu no jumyo to NO{sub x} haishutsuryo no kaizen

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawa, Tsuguhiko; Nuta, Kunihiro [Kawasaki Steel Corp., Okayama, (Japan). Mizushima Warks

    1999-03-10

    The radiant tube heating system has been widely applied to the furnaces which require isolation of the heating atmosphere from the combustion atmosphere. However, the conventional system has a short life and it is difficult to reduce NO{sub x} emission when it is used at a high furnace temperature under high combustion load, because the fuel is burned in a small space. In order to solve this problem, we have studied the cause of radiant tube life depends on the uniformity of the temperature distribution along the radiant tube. We have developed a new burner using a two-stage combustion method with exhaust gas self-recirculation. As a result, the file of the new system has been increased by a factor of two or more, and NO{sub x} emission has been reduced by 20 % from previous levels. This paper presents an outline of the elastic-plastic creep analysis and the new burner, and describes the effect of its use on system life. (author)

  10. Metabolism of sulfate-reducing bacteria and corrosion behavior of carbon steel in the continuous culturing medium; Renzoku baiyo baichichu ni okeru ryusan`en kangen no taisha to tansoko no fushoku kyodo

    Energy Technology Data Exchange (ETDEWEB)

    Baba, F.; Suzuki, T. [Ajinomoto Co. Inc., Tokyo (Japan); Seo, M. [Hokkaido University, Sapporo (Japan)

    1997-08-25

    Investigations were made on metabolism of sulfate-reducing bacteria and corrosion behavior of carbon steel in the continuous culturing medium. Sulfate-reducing bacteria were cultured for 50 days by supplying the culturing medium prepared to a prescribed chemical composition (containing Fe {sup 2+} at 0.01 mol/kg) at a rate of 10 cm {sup 3}/h, and drawing them out at the same rate. Test carbon steel pieces were immersed into this culturing medium. As a result, the following matters were clarified: the number of bacteria is maintained at more than 10 {sup 10}/cm{sup 3} after several days since inauguration of the immersion, with the bacteria stably producing H2S and FeS until the culturing is finished; comma-shaped bacteria which move actively and rod-shaped bacteria which do not move very actively exist in the culturing medium; a black film has been produced on surface of the test pieces throughout the culturing period, and satin-like corrosion was found underneath the surface; and weight increase of this film and weight decrease of the lower layer progress as the time lapses (the weight decrease of the lower layer has reached 40 mg/cm{sup 2} in 50 days). 28 refs., 8 figs., 1 tab.

  11. Characteristics of mixed culture where one type of microorganism assimilates the metabolite; Isshurui no biseibutsu ga seiseisuru taishabutsu wo betsuno shuruino biseibutsu ga shikashite zoshokusuru kongo baiyo no moderuka to tokusei kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Toyama, M.; Matsunaka, T.; Shimizu, K. [Kyushu Inst. of Tech., Fukuoka (Japan). Dept. of Biochemical Engineering and Science

    2000-11-10

    Unstructure models were developed for a mixed culture where one microorganism assimilates the metabolite produced by another microorganism The model system was a mixed culture using Lactobacillus delbrueckii and Ralstonia erthroprop where the former assimilates glucose and produces lactic acid, and the latter assimilates lactate and produce poly {beta}-hydroxy butyrate (PHB). Performance improvement is shown for the mixed culture over a single culture for chemostat using the model developed. Since the optimal dissolved oxygen (DO) concentration is different for each microorganism, we developed another model which takes into account the effect of DO concentration on the dynamic behavior. Then the optimal DO concentration is obtained for chemostat. Moreover, we developed another model which takes into account NH{sub 3} concentration on the cell grown and PHB production by R. eutropha. Then the optimal time-profile for NH{sub 3} concentration is derived using the maximum principle. It is found that high Phba production could be attained even if NH{sub 3} concentration is not controlled if initial NH{sub 3} concentration is appropriately selected. (author)

  12. Estimation of network structure for signal propagations by the analysis of multichannel action potentials in cultured neural networks; Ta channel katsudo den`i kaiseki ni yoru baiyo shinkei kairomonai kofun denpa keiro no suitei

    Energy Technology Data Exchange (ETDEWEB)

    Konno, N.; Fukami, T.; Shiina, T. [University of Tsukuba, Tsukuba (Japan); Jinbo, Y. [Nippon Telegraph and Telephone Corp., Tokyo (Japan)

    1998-07-01

    We have fabricated a 64 embedded microelectrode-array substrate using semiconductor technology to investigate the biological signal processing in brain by using cultured neural networks of fetal rat neocortex in vitro. We analyzed temporal and spatial neural networks patterns cultured on electrode-array substrate and attempted to examine the network structure constituted by neurons and the propagating patterns of electrical activity induced by the electric stimulus. In the experiments, each microelectrode size was 30 {mu}m squared and 150{mu} m spaced. For stimulation, one of the electrodes was selected and current pulses were applied through an isolated circuit. After the network was cultured in about 50 days, responses of neurons to electric stimulus were monitored extracellularly through 64-channel electrode array. Data recorded at each electrode consist of several spike trains generated by different cells. Therefore, these trains were separated by using wavelet transform and template matching for each electrode. We referred the temporal patterns of generated spikes for each electrode to as `spike sequences`. Next, we compared With the spike sequences among multichannel data and visualized the Cultured neural networks structure by identifying the directions of propagations and cell connections. 15 refs., 9 figs.

  13. Study on utilization of shallow sea area by building an alga field. Discussions on its utilization for cultivating Ulvales; Sojo zosei ni yoru senkaiiki riyo no kenkyu. Anaaosa no baiyo to sono riyo ni kansuru kento

    Energy Technology Data Exchange (ETDEWEB)

    Tsuno, M.; Maebayashi, M. [Hokkaido Electric Power Co. Inc., Sapporo (Japan)

    1997-03-25

    Discussions were given on mass cultivation in a water tank using sea water of an alga Ulvales used as feed for sea urchins and abalone. A fertilizer composed of ammonium sulfate and superphosphate of lime was used, and a water temperature was set as the environmental condition. Two kinds of Ulvales produced in different areas were used. One of the two kinds had full growth periodical and weight increasing rate low, but the other Ulvales had high weight increasing rate and no blights in alga bodies, and was found suitable for mass production. The result of a test on stocks of the latter species may be described as follows: multiple regression formulas for harvest, water temperature and illumination intensity were introduced for each cultivating condition for four cases regarding accommodation density and harvest frequency; and if a cultivating condition by which the harvest is maximized is adopted, a one cubic meter water tank placed on a place with good sunlight may be expected of producing as much harvest as 2181 grams at water temperature of 15{degree}C and 4899 grams at 25{degree}C. The cultivation requires the following precautions: aeration is required; sea water should be introduced into the tank; a mixed fertilizer with ratio of ammonium sulfate at 100 to superphosphate of lime at 15 may be used; water temperatures suitable for mass cultivation are 13 to 15{degree}C or higher; and light must be irradiated evenly. 14 refs., 9 figs., 5 tabs.

  14. Leading research report for fiscal 1998. Research and study of 3-dimensional cell structure module engineering; 1998 nendo sendo chosa kenkyu hokokusho. Sanjigen saibo soshiki module kogaku chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    For the formation of cellular tissues to replace bionic tissues, researches were conducted about technologies of forming bionic tissue modules by culturing various kinds of cells. As for the materials and methods for constructing cellular tissues, researches were conducted about the trends of research and development of 3-dimensional tissue culturing matrices and materials for micromanipulation. As for the development of technologies for the functionalization of 3-dimensionally structured cells, research and study were conducted about the technology of 3-dimensional cell structure organization through application of physical stimulation, the biochemical technology of differentiation inducing, and the differentiation inducing technology for hetero tissue culturing. As for the development of technologies for evaluation using 3-dimensionally structured cells, light CT (computer tomography), analysis and evaluation using spectroscopy and the like, feasibility of the biochemical analysis of the cell state using biosensors, technologies for measuring the secretion of carcinogenic and toxic substances, etc., were studied. In addition, the development of organic models to replace test animals, industrial evolution of 3-dimensional tissue module engineering, etc., were investigated. (NEDO)

  15. Leading research report for fiscal 1998 on the research of basic technologies of cranial nerve cell engineering; 1998 nendo noshinkei saibo kogaku kiban gijutsu no chosa kenkyu sendo kenkyu hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Researches are conducted about the present and future of the research and development of elementary technological details for elucidating, from the viewpoint of the level of molecules or cells, the functions of neurons which are the basic elements of the cranial nerve circuit and for applying the results to cranial nerve cell engineering. It is then concluded that the most important and urgent is to 'develop basic technologies for understanding the functions of neural circuits so that a remedial step may be taken in the presence of a defect in a neural circuit and that electronic devices may be applied to help neural functions.' It is agreed that the most immediate and ripple-effective is to 'establish the technology of intelligent connection between electronic devices and neural circuits for the reconstruction of motor functions.' It is also concluded that, upon achievement of the above two goals, new basic technologies will become available for the moderation of problems in the sensor input and motor output systems by means of electronic circuits, for the improvement on efficiency of rehabilitation with the aid of electronics, for the cure of denatured nerves or a damaged spinal cord, for the study of neural circuits, etc., and that a new industrial series will be created through the utilization of the above-mentioned details. (NEDO)

  16. Leading research report for fiscal 1998 on the research of basic technologies of cranial nerve cell engineering; 1998 nendo noshinkei saibo kogaku kiban gijutsu no chosa kenkyu sendo kenkyu hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Researches are conducted about the present and future of the research and development of elementary technological details for elucidating, from the viewpoint of the level of molecules or cells, the functions of neurons which are the basic elements of the cranial nerve circuit and for applying the results to cranial nerve cell engineering. It is then concluded that the most important and urgent is to 'develop basic technologies for understanding the functions of neural circuits so that a remedial step may be taken in the presence of a defect in a neural circuit and that electronic devices may be applied to help neural functions.' It is agreed that the most immediate and ripple-effective is to 'establish the technology of intelligent connection between electronic devices and neural circuits for the reconstruction of motor functions.' It is also concluded that, upon achievement of the above two goals, new basic technologies will become available for the moderation of problems in the sensor input and motor output systems by means of electronic circuits, for the improvement on efficiency of rehabilitation with the aid of electronics, for the cure of denatured nerves or a damaged spinal cord, for the study of neural circuits, etc., and that a new industrial series will be created through the utilization of the above-mentioned details. (NEDO)

  17. Toxicity assessment of organochlorine compounds detected in water environment using cultured human cell lines; Hito yurai saibo baiyokei wo mochiita suikankyo shiryochu no yuki enso kagobutsu no dokusei hyoka

    Energy Technology Data Exchange (ETDEWEB)

    Kunimoto, M; Yonemoto, J; Soma, Y; Nakasugi, O [National Institute for Environmental Studies, Tsukuba (Japan)

    1997-11-10

    As part of validation processes of in vitro toxicity assays for the risk assessment of environmental hazards, we applied an in vitro toxicity test using two human cell lines, neuroblastoma NB-1 cells and glioblastoma U-87 MG cells, to the assessment of organochlorine compounds detected in the water environment. The in vitro toxicity assay using NB-1 cells was calibrated by testing reference chemicals proposed by MEIC (Multicenter Evaluation of In Vitro Cytotoxicity), an international program for the validation of in vitro cytotoxicity assays. Beforehand, an assay using cells in frozen stock without subcultivation was examined by comparing IC50 values with the ordinary assay using subcultured cells. IC50 values for MEIC reference chemicals from the former assay showed good correlation with those from the latter assay, suggesting that the assay using cells in frozen stock can be used at least for the assessment of basal cytotoxicity. IC50 values for ten organochlorine compounds frequently detected in the sediment samples from contaminated rivers, p-chloroaniline, 3,4-dichloroaniline, p-dichlorobenzene, o-dichlorobenzene, Tris (2-chloroethyl)-phosphate, 2,5-dichlorophenol, 2,5-dichloroanisol, Triclosan and Triclocarban, were obtained with the in vitro assays and compared with their LD50 values in rats. No significant correlation, however, was seen between the IC50 and LD50 values, indicating that further improvement of in vitro toxicity assays is necessary for the application to the risk assessment of environmental hazards. 7 refs., 4 figs., 1 tab.

  18. FY 1997 report on the survey of fundamental technologies in the field of brain neuro-biotechnology; 1997 nendo Sendo kenkyu hokokusho (noshinkei saibo kogaku kiban gijutsu no chosa kenkyu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    In Japan with its rapidly aging society, fundamental technologies are required for the development of artificial nerves substituting for or supporting damaged ones, and ultimately constituting artificial neurons based on the knowledge of the brain functions at the molecular and cellular levels. This study defines the fundamental technologies which would be required for the development in the area, and further, evaluates the potential of the technologies to develop the novel industry. The brain function is closely related to the activity in neuronal circuits. In order to repair injured nerves and to develop the advanced technologies of electronics for helping impaired neuronal functions, the most important and urgent is to understand how to work the neuronal circuit system in the brain. Based on these viewpoints, new methodological approaches would make it possible to relieve neural impairment in the sensory input system and the motor system by the use of electronic circuits. They also would improve rehabilitation after injury, and treat neurodegenerative diseases such as Parkinson`s disease. These advances surely create the new types of industry seeds in near future. 77 refs., 29 figs., 2 tabs.

  19. Improvement on life and NO{sub x} discharge of radiant heat transfer tube heating system by the elasto-plasticity creep analysis; Dansosei kuripukaiseki ni yoru hosha dennetsukan kanetsu shisutemu no jumyo to NO{sub x} haishutsuryo no kaizen

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawa, Futahiko; Ikaruda, Kunihiro; Abe, Yoshio; Arai, Norio

    1999-06-05

    Combustion thermal process using the radiant heat transfer tube has widely been applied as a heating method which separates the combustion atmosphere from the heating-e atmosphere in various heating furnace such as iron and steel industry. In this thermal process, in order to burn the fuel in tight space in radiant heat transfer service area, radiant heat transfer tube and burner life were short under high temperature and high-load combustion, and there was a problem that that and, burning characteristic such as NO{sub x} generation rate are improved was difficult. In this study, large temperature distribution by the combustion in the radiant heat transfer tube clarified that the life of the radiant heat transfer tube was shortened by elasto-plasticity creep analysis of the radiant heat transfer tube. Then, two steps combustion burner of the exhaust gas self recycling type was developed as a method for reducing the NO{sub x} generation rate, while the temperature distribution of the radiant heat transfer tube was equalized. As the result, it was possible to reduce over 20% in comparison with conventional two steps combustion burner, while radiant heat transfer tube and life of the burner are extended over the conventional double, in respect of the NO{sub x} generation rate. (translated by NEDO)

  20. Report on achievements in fiscal 1998. Venture business growing type consortium - small business creating infrastructure (Development of ultra-high efficiency cultivation system for Hyphomycetes, and application of the system to plant device to manufacture fermentative foods); 1998 nendo itojokin no chokoritsu tairyo baiyo system no kaihatsu to kinosei hakko sho0kuhin seizo plant kiki eno tekiyo seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    This project has noticed the membrane surface liquid culture method, and researched and developed the following themes with an objective to commercialize the membrane surface liquid culture system and develop novel fermentative foods using this system. Cultivation of Beni-koji bacteria (solid culture of M. pilosus k) is taken up in the model cultivation system. (1) establishment of the membrane surface liquid culture system and development of devices, (2) elucidation of cultivation characteristics of the membrane surface liquid culture system and optimization of the cultivating conditions, and (3) development of fermentative foods. In Item (1), such problems were solved as automation of bacteria planting, long-term pasteurized cultivation, and assurance of clean cultivation broth. In Item (2), it was discovered most suitable that maltose concentration is about 5%, glutamic acid concentration is about 50 mM, the initial culture medium pH is about six, and the cultivation temperature is 30 degree C. In item (3), as a result of mixing this red coloring matter containing cultivation broth into preparation of Miso (bean paste) and soy sauce, products having high {gamma}-Aminobutyric acid and monacholin k content were developed. In addition, while Beni-koji made by solid cultivation had peculiar taste and odor, this drawback was eliminated by using this coloring matter containing cultivation broth. This has made differentiation from conventional products possible. (NEDO)

  1. Typing of the sausage-shaped bacteria forming A-type sulfur-turf according to cell length distributions of natural populations and physico-chemical conditions of hot spring waters; Saibo chobunpu to seiiku kankyo kara mita A gata io shiba shizen kotaigun ni okeru okamagata saikin no katabetsu ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Aki, Y. [Iwate University, Iwate (Japan). Faculty of Humanities and Social Sciences

    1996-01-25

    In order to type the sausage-shaped bacteria forming A-type sulfur-turf, cell length distributions and physics-chemical conditions of hot spring waters were investigated on twelve samples collected from all over Japan. The frequency distributions of the cell length of eight samples were bimodal, while the other four samples were unimodal. In seven samples with bimodal distributions, two types (large and small) of the sausage-shaped bacteria could be differentiated. The cell length of the large-type was between 10.1 and 31.9{mu}m, while that of the small-type ranged 2.2 to 6.6{mu}m. The pH of seven hot spring waters were between 6 and 8, and the two types (large and small) formed together sulfur-turf. In contrast, pH of the three hot springs were over 8, and the two types of the sausage-shaped bacteria could not be detected in the sulfur-turf. Therefore, it is reasonable to set a third type of the sausage-shaped bacteria which prefers high-pH (over 8) and low calcium condition. The cell lengths of the third type were in the range of 5.5 to 8.6{mu}m, which correspond to the sausage-shaped bacteria of medium size. 20 refs., 5 figs., 2 tabs.

  2. CEIBA: a fast track plan with multiphase pumping by 750 m of water; CEIBA: un projet fast track avec pompage multiphasique par 750 m d'eau. Canyon Express: le plus profond des reseaux de production. Le Saibos FDS: un navire polyvalent adapte au defit des developements en grande profondeur

    Energy Technology Data Exchange (ETDEWEB)

    Delaporte, M. [Paragon Litwin, 92 - Nanterre (France); Obsen, M. [Framo Engineering, Bergen (Norway); Bang, P.; RIjkens, F. [Total, La Defense 6, 92 - Courbevoie (France); Poirson, L. [Saibos sas, 78 - Guyancourt (France)

    2003-08-01

    The development of the field of CEIBA, located at 22 miles off the equatorial Guinea coast, has been the aim of a presentation AFTP/SPE on May 21 at Clamart. M. Delaporte, director of offshore study and development of projects at Paragon Litwin has presented at first an overview of the plan and then M. Olsen, commercial responsible of Framo Engineering has given precision on the polyphasic pumping equipments which particularize this development. (O.M.)

  3. Entering 'A NEW REALM' of KIBO Payload Operations - Continuous efforts for microgravity experiment environment and lessons learned from real time experiment operations in KIBO -

    International Nuclear Information System (INIS)

    Sakagami, K; Goto, M; Matsumoto, S; Ohkuma, H

    2011-01-01

    On January 22nd, 2011(JST), KOUNOTORI2 (H-II Transfer Vehicle: HTV2) was successfully launched from Tanegashima Space Center toward the International Space Station (ISS) and two new JAXA payload racks, Kobairo rack and MSPR (Multi-purpose Small Payload Rack) were transferred to ISS/KIBO (Japanese Experiment Module: JEM). In addition to Saibo rack and Ryutai rack which are already in operation in KIBO, in total 4 Japanese experiment payload racks start operations in KIBO. Then KIBO payload operations embark on a new realm, full utilization phase. While the number and variety of microgravity experiments become increasing, simultaneous operation constraints should be considered to achieve multitask payload operations in ISS/KIBO and ever more complicated cooperative operations between crewmember and flight control team/science team are required. Especially for g-jitter improvement in ISS/KIBO, we have greatly advanced cooperative operations with crewmember in the recent increment based on the microgravity data analysis results. In this paper, newly operating Japanese experiment payloads characteristics and some methods to improve g-jitter environment are introduced from the front line of KIBO payload operations.

  4. Assessing Pearl Quality Using Reflectance UV-Vis Spectroscopy: Does the Same Donor Produce Consistent Pearl Quality?

    Directory of Open Access Journals (Sweden)

    Paul C. Southgate

    2010-09-01

    Full Text Available Two groups of commercial quality (“acceptable” pearls produced using two donors, and a group of “acceptable” pearls from other donors were analyzed using reflectance UV-Vis spectrophotometry. Three pearls with different colors produced by the same donor showed different absorption spectra. Cream and gold colored pearls showed a wide absorption from 320 to about 460 nm, while there was just slight reflectance around 400 nm by the white pearl with a pink overtone. Cream and gold pearls reached a reflectance peak at 560 to 590 nm, while the white pearl with pink overtone showed slightly wider absorption in this region. Both cream and gold pearls showed an absorption peak after the reflectance peak, at about 700 nm for the cream pearl and 750 nm for the gold pearl. Two other pearls produced by the same donor (white with cream overtone and cream with various overtones showed similar spectra, which differed in their intensity. One of these pearls had very high lustre and its spectrum showed a much higher percentage reflectance than the second pearl with inferior lustre. This result may indicate that reflectance is a useful quantitative indicator of pearl lustre. The spectra of two white pearls resulting from different donors with the same color nacre (silver showed a reflectance at 260 nm, followed by absorption at 280 nm and another reflectance peak at 340 nm. After this peak the spectra for these pearls remained flat until a slight absorption peak around 700 nm. Throughout the visible region, all white pearls used in this study showed similar reflectance spectra although there were differences in reflectance intensity. Unlike the spectral results from white pearls, the results from yellow and gold pearls varied according to color saturation of the pearl. The results of this study show that similarities between absorption and reflectance spectra of cultured pearls resulting from the same saibo donor are negligible and could not be detected