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Sample records for sacred lotus nelumbo

  1. Thermal-stable proteins of fruit of long-living Sacred Lotus Nelumbo nucifera Gaertn var. China Antique.

    Science.gov (United States)

    Shen-Miller, J; Lindner, Petra; Xie, Yongming; Villa, Sarah; Wooding, Kerry; Clarke, Steven G; Loo, Rachel R O; Loo, Joseph A

    2013-09-01

    Single-seeded fruit of the sacred lotus Nelumbo nucifera Gaertn var. China Antique from NE China have viability as long as ~1300 years determined by direct radiocarbon-dating, having a germination rate of 84%. The pericarp, a fruit tissue that encloses the single seeds of Nelumbo , is considered one of the major factors that contribute to fruit longevity. Proteins that are heat stable and have protective function may be equally important to seed viability. We show proteins of Nelumbo fruit that are able to withstand heating, 31% of which remained soluble in the 110°C-treated embryo-axis of a 549-yr-old fruit and 76% retained fluidity in its cotyledons. Genome of Nelumbo is published. The amino-acid sequences of 11 "thermal proteins" (soluble at 100°C) of modern Nelumbo embryo-axes and cotyledons, identified by mass spectrometry, Western blot and bioassay, are assembled and aligned with those of an archaeal-hyperthermophile Methancaldococcus jannaschii (Mj; an anaerobic methanogen having a growth optimum of 85°C) and with five mesophile angiosperms. These thermal proteins have roles in protection and repair under stress. More than half of the Nelumbo thermal proteins (55%) are present in the archaean Mj, indicating their long-term durability and history. One Nelumbo protein-repair enzyme exhibits activity at 100°C, having a higher heat-tolerance than that of Arabidopsis. A list of 30 sequenced but unassembled thermal proteins of Nelumbo is supplemented.

  2. The genome of the long-living Sacred lotus (Nelumbo nucifera, Gaertn.)

    Science.gov (United States)

    Sacred lotus, a basal eudicot of agricultural, medicinal, cultural, and religious importance, is particularly noted for its 1,300-year seed longevity, and for its outstanding water repellency, the "lotus effect”. The genome sequence of the Sacred lotus variety 'China Antique' lacks the paleo-triplic...

  3. Genome of the long-living sacred lotus (Nelumbo nucifera Gaertn.).

    Science.gov (United States)

    Ming, Ray; VanBuren, Robert; Liu, Yanling; Yang, Mei; Han, Yuepeng; Li, Lei-Ting; Zhang, Qiong; Kim, Min-Jeong; Schatz, Michael C; Campbell, Michael; Li, Jingping; Bowers, John E; Tang, Haibao; Lyons, Eric; Ferguson, Ann A; Narzisi, Giuseppe; Nelson, David R; Blaby-Haas, Crysten E; Gschwend, Andrea R; Jiao, Yuannian; Der, Joshua P; Zeng, Fanchang; Han, Jennifer; Min, Xiang Jia; Hudson, Karen A; Singh, Ratnesh; Grennan, Aleel K; Karpowicz, Steven J; Watling, Jennifer R; Ito, Kikukatsu; Robinson, Sharon A; Hudson, Matthew E; Yu, Qingyi; Mockler, Todd C; Carroll, Andrew; Zheng, Yun; Sunkar, Ramanjulu; Jia, Ruizong; Chen, Nancy; Arro, Jie; Wai, Ching Man; Wafula, Eric; Spence, Ashley; Han, Yanni; Xu, Liming; Zhang, Jisen; Peery, Rhiannon; Haus, Miranda J; Xiong, Wenwei; Walsh, James A; Wu, Jun; Wang, Ming-Li; Zhu, Yun J; Paull, Robert E; Britt, Anne B; Du, Chunguang; Downie, Stephen R; Schuler, Mary A; Michael, Todd P; Long, Steve P; Ort, Donald R; Schopf, J William; Gang, David R; Jiang, Ning; Yandell, Mark; dePamphilis, Claude W; Merchant, Sabeeha S; Paterson, Andrew H; Buchanan, Bob B; Li, Shaohua; Shen-Miller, Jane

    2013-05-10

    Sacred lotus is a basal eudicot with agricultural, medicinal, cultural and religious importance. It was domesticated in Asia about 7,000 years ago, and cultivated for its rhizomes and seeds as a food crop. It is particularly noted for its 1,300-year seed longevity and exceptional water repellency, known as the lotus effect. The latter property is due to the nanoscopic closely packed protuberances of its self-cleaning leaf surface, which have been adapted for the manufacture of a self-cleaning industrial paint, Lotusan. The genome of the China Antique variety of the sacred lotus was sequenced with Illumina and 454 technologies, at respective depths of 101× and 5.2×. The final assembly has a contig N50 of 38.8 kbp and a scaffold N50 of 3.4 Mbp, and covers 86.5% of the estimated 929 Mbp total genome size. The genome notably lacks the paleo-triplication observed in other eudicots, but reveals a lineage-specific duplication. The genome has evidence of slow evolution, with a 30% slower nucleotide mutation rate than observed in grape. Comparisons of the available sequenced genomes suggest a minimum gene set for vascular plants of 4,223 genes. Strikingly, the sacred lotus has 16 COG2132 multi-copper oxidase family proteins with root-specific expression; these are involved in root meristem phosphate starvation, reflecting adaptation to limited nutrient availability in an aquatic environment. The slow nucleotide substitution rate makes the sacred lotus a better resource than the current standard, grape, for reconstructing the pan-eudicot genome, and should therefore accelerate comparative analysis between eudicots and monocots.

  4. Alkaloid constituents from flower buds and leaves of sacred lotus (Nelumbo nucifera, Nymphaeaceae) with melanogenesis inhibitory activity in B16 melanoma cells.

    Science.gov (United States)

    Nakamura, Seikou; Nakashima, Souichi; Tanabe, Genzo; Oda, Yoshimi; Yokota, Nami; Fujimoto, Katsuyoshi; Matsumoto, Takahiro; Sakuma, Rika; Ohta, Tomoe; Ogawa, Keiko; Nishida, Shino; Miki, Hisako; Matsuda, Hisashi; Muraoka, Osamu; Yoshikawa, Masayuki

    2013-02-01

    Methanolic extracts from the flower buds and leaves of sacred lotus (Nelumbo nucifera, Nymphaeaceae) were found to show inhibitory effects on melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells. From the methanolic extracts, a new alkaloid, N-methylasimilobine N-oxide, was isolated together with eleven benzylisoquinoline alkaloids. The absolute stereostructure of the new alkaloid was determined from chemical and physicochemical evidence. Among the constituents isolated, nuciferine, N-methylasimilobine, (-)-lirinidine, and 2-hydroxy-1-methoxy-6a,7-dehydroaporphine showed potent inhibition of melanogenesis. Comparison of the inhibitory activities of synthetic related alkaloids facilitated characterization of the structure-activity relationships of aporphine- and benzylisoquinoline-type alkaloids. In addition, 3-30 μM nuciferine and N-methylasimilobine inhibited the expression of tyrosinase mRNA, 3-30 μM N-methylasimilobine inhibited the expression of TRP-1 mRNA, and 10-30 μM nuciferine inhibited the expression of TRP-2 mRNA. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. The Sacred Lotus

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 18; Issue 8. The Sacred Lotus - An Incredible Wealth of Wetlands. R N Mandal R Bar. General Article Volume 18 Issue 8 August 2013 pp 732-737. Fulltext. Click here to view fulltext PDF. Permanent link:

  6. The Sacred Lotus

    Indian Academy of Sciences (India)

    IAS Admin

    The lotus is very much a part of Indian cultural traditions. Its importance has long been recognized and it has been grown for use as food, medicine and also for cultural and religious activities. Significantly, the longevity of the lotus seed is phenomenal, with viable seeds dated as being 1300 years old. Seed germination of ...

  7. The Sacred Lotus

    Indian Academy of Sciences (India)

    IAS Admin

    its petals and emitting fragrance as soon as the sun rises, whereas. Kamal means that the presence of the lotus makes the water body vivacious [3]. Pankaja means 'growing in mud' which is consid- ered to be a humble origin, but even so it ascends untainted above the water body with a flower of great aesthetic beauty.

  8. Flavonoids of Lotus (Nelumbo nucifera) Seed Embryos and Their Antioxidant Potential.

    Science.gov (United States)

    Zhu, Mingzhi; Liu, Ting; Zhang, Chunyun; Guo, Mingquan

    2017-08-01

    Flavonoids from lotus (Nelumbo nucifera) seed embryos were fractionated over a macroporous resin chromatography into 2 main fractions (I and II), and subsequently identified by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS 2 ). Sixteen flavonoids were identified in lotus seed embryos, including 8 flavonoid C-glycosides and 8 flavonoid O-glycosides, in which the flavonoid C-glycosides were the main flavonoids. Among them, 2 flavonoid O-glycosides (luteolin 7-O-neohesperidoside and kaempferol 7-O-glucoside) were identified in lotus seed embryos for the 1st time. For further elucidating the effects of flavonoid C-glycosides to the bioactivities of lotus seed embryos, we compared the differences of the flavonoids and their antioxidant activities between leaves and seed embryos of lotus using the same methods. The results showed the antioxidant activity of flavonoids in lotus seed embryos was comparable or higher than that in lotus leaves, whereas the total flavonoid content in seed embryos was lower than lotus leaves which only contained flavonoid O-glycosides. The flavonoid C-glycosides of lotus seed embryos had higher antioxidant properties than the flavonoid O-glycosides presented in lotus leaves. This study suggested that the lotus seed embryos could be promising sources with antioxidant activity and used as dietary supplements for health promotion. © 2017 Institute of Food Technologists®.

  9. Characterization of flower-bud transcriptome and development of genic SSR markers in Asian lotus (Nelumbo nucifera Gaertn..

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    Weiwei Zhang

    Full Text Available Asian lotus (Nelumbo nucifera Gaertn. is the national flower of India, Vietnam, and one of the top ten traditional Chinese flowers. Although lotus is highly valued for its ornamental, economic and cultural uses, genomic information, particularly the expressed sequence based (genic markers is limited. High-throughput transcriptome sequencing provides large amounts of transcriptome data for promoting gene discovery and development of molecular markers.In this study, 68,593 unigenes were assembled from 1.34 million 454 GS-FLX sequence reads of a mixed flower-bud cDNA pool derived from three accessions of N. nucifera. A total of 5,226 SSR loci were identified, and 3,059 primer pairs were designed for marker development. Di-nucleotide repeat motifs were the most abundant type identified with a frequency of 65.2%, followed by tri- (31.7%, tetra- (2.1%, penta- (0.5% and hexa-nucleotide repeats (0.5%. A total of 575 primer pairs were synthesized, of which 514 (89.4% yielded PCR amplification products. In eight Nelumbo accessions, 109 markers were polymorphic. They were used to genotype a sample of 44 accessions representing diverse wild and cultivated genotypes of Nelumbo. The number of alleles per locus varied from 2 to 9 alleles and the polymorphism information content values ranged from 0.6 to 0.9. We performed genetic diversity analysis using 109 polymorphic markers. A UPGMA dendrogram was constructed based on Jaccard's similarity coefficients revealing distinct clusters among the 44 accessions.Deep transcriptome sequencing of lotus flower buds developed 3,059 genic SSRs, making a significant addition to the existing SSR markers in lotus. Among them, 109 polymorphic markers were successfully validated in 44 accessions of Nelumbo. This comprehensive set of genic SSR markers developed in our study will facilitate analyses of genetic diversity, construction of linkage maps, gene mapping, and marker-assisted selection breeding for lotus.

  10. Identification of a R2R3-MYB gene regulating anthocyanin biosynthesis and relationships between its variation and flower color difference in lotus (Nelumbo Adans.

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    Shan-Shan Sun

    2016-09-01

    Full Text Available The lotus (Nelumbonaceae: Nelumbo Adans. is a highly desired ornamental plant, comprising only two extant species, the sacred lotus (N. nucifera Gaerten. with red flowers and the American lotus (N. lutea Willd. with yellow flowers. Flower color is the most obvious difference of two species. To better understand the mechanism of flower color differentiation, the content of anthocyanins and the expression levels of four key structural genes (e.g., DFR, ANS, UFGT and GST were analyzed in two species. Our results revealed that anthocyanins were detected in red flowers, not yellow flowers. Expression analysis showed that no transcripts of GST gene and low expression level of three UFGT genes were detected in yellow flowers. In addition, three regulatory genes (NnMYB5, NnbHLH1 and NnTTG1 were isolated from red flowers and showed a high similarity to corresponding regulatory genes of other species. Sequence analysis of MYB5, bHLH1 and TTG1 in two species revealed striking differences in coding region and promoter region of MYB5 gene. Population analysis identified three MYB5 variants in Nelumbo: a functional allele existed in red flowers and two inactive forms existed in yellow flowers. This result revealed that there was an association between allelic variation in MYB5 gene and flower color difference. Yeast two-hybrid experiments showed that NnMYB5 interacts with NnbHLH1, NlbHLH1 and NnTTG1, and NnTTG1 also interacts with NnbHLH1 and NlbHLH1. The over-expression of NnMYB5 led to anthocyanin accumulation in immature seeds and flower stalks and up-regulation of expression of TT19 in Arabidopsis. Therefore, NnMYB5 is a transcription activator of anthocyanin synthesis. This study helps to elucidate the function of NnMYB5 and will contribute to clarify the mechanism of flower coloration and genetic engineering of flower color in lotus.

  11. Identification of a R2R3-MYB gene regulating anthocyanin biosynthesis and relationships between its variation and flower color difference in lotus (Nelumbo Adans.).

    Science.gov (United States)

    Sun, Shan-Shan; Gugger, Paul F; Wang, Qing-Feng; Chen, Jin-Ming

    2016-01-01

    The lotus (Nelumbonaceae: Nelumbo Adans.) is a highly desired ornamental plant, comprising only two extant species, the sacred lotus (N. nucifera Gaerten.) with red flowers and the American lotus (N. lutea Willd.) with yellow flowers. Flower color is the most obvious difference of two species. To better understand the mechanism of flower color differentiation, the content of anthocyanins and the expression levels of four key structural genes (e.g., DFR, ANS, UFGT and GST) were analyzed in two species. Our results revealed that anthocyanins were detected in red flowers, not yellow flowers. Expression analysis showed that no transcripts of GST gene and low expression level of three UFGT genes were detected in yellow flowers. In addition, three regulatory genes (NnMYB5, NnbHLH1 and NnTTG1) were isolated from red flowers and showed a high similarity to corresponding regulatory genes of other species. Sequence analysis of MYB5, bHLH1 and TTG1 in two species revealed striking differences in coding region and promoter region of MYB5 gene. Population analysis identified three MYB5 variants in Nelumbo: a functional allele existed in red flowers and two inactive forms existed in yellow flowers. This result revealed that there was an association between allelic variation in MYB5 gene and flower color difference. Yeast two-hybrid experiments showed that NnMYB5 interacts with NnbHLH1, NlbHLH1 and NnTTG1, and NnTTG1 also interacts with NnbHLH1 and NlbHLH1. The over-expression of NnMYB5 led to anthocyanin accumulation in immature seeds and flower stalks and up-regulation of expression of TT19 in Arabidopsis. Therefore, NnMYB5 is a transcription activator of anthocyanin synthesis. This study helps to elucidate the function of NnMYB5 and will contribute to clarify the mechanism of flower coloration and genetic engineering of flower color in lotus.

  12. Factors influencing fecundity in experimental crosses of water lotus (Nelumbo nucifera Gaertn. cultivars

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    Teng Nian-Jun

    2012-06-01

    Full Text Available Abstract Background Breeding programs for the water lotus (Nelumbo nucifera are hampered by an inability to account for variation in seed set associated with crosses between different cultivars. We studied seed set in two reciprocal crosses between lotus cultivars (‘Guili’ × ‘Aijiangnan’ and ‘Molingqiuse’ × ‘Qinhuaiyanzhi' to obtain insights into factors that govern fecundity in these experimental hybrids. Pollen viability, stigma receptivity and embryo development were compared for each hybrid and reciprocal cross. Results Pollen viability of the individual cultivars ranged from 4.1% to 20.2%, with the highest level (>11.9% for all cultivars observed from the earliest collected grains (05:00–06:00 a.m.. Stigmatic pollen germination peaked at 4 h after pollination and varied from 4.8 to 60.6 grains per stigma among the crosses. Production of normal embryos ranged from 7.6% to 58.8% at 1 d after pollination and from 0 to 25% by 11 d after pollination. Seed set in crosses (0.2–23.3% was generally lower than in open-pollinated plants (8.4–26.5%. Similar to the germination results, seed set was substantially reduced in both reciprocal crosses. Conclusions These results suggested that poor pollen fertility, low stigma receptivity, and embryo abortion were responsible for the failure of the crosses ‘Molingqiuse’ × ‘Qinhuaiyanzhi’, ‘Qinhuaiyanzhi’ × ‘Molingqiuse’, and ‘Aijiangnan’ × ‘Guili’.

  13. Changes in Drosophila melanogaster Sleep-Wake Behavior Due to Lotus (Nelumbo nucifera) Seed and Hwang Jeong (Polygonatum sibiricum) Extracts

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    Jo, Kyungae; Jeon, SangDuk; Ahn, Chang-Won; Han, Sung Hee; Suh, Hyung Joo

    2017-01-01

    We evaluated the sleep enhancement activity of the medicinal herbs valerian (Valeriana officinalis), jujube (Ziziphus jujube), lotus seed (Nelumbo nucifera), Gastrodia elata, Polygonatum sibiricum, and baekbokryung (Poria cocos), which can relieve insomnia in a Drosophila model. Locomotor activity was measured in the Drosophila model to evaluate the sleep activity of Korean medicinal herbs traditionally used as sleep aids. The group treated with lotus seed extract showed less nocturnal activity. Treatment with 10 or 20 mg/mL of P. sibiricum significantly reduced nocturnal activity compared to the control group (P<0.05). The activity and sleep bouts of fruit flies were significantly decreased by a high-dose treatment (10 mg/mL) of lotus or P. sibiricum extracts at night. Caffeine-treated Drosophila showed increased nocturnal activity and decreased total sleep time (P<0.05). Flies receiving the 10 mg-doses of lotus seed or P. sibiricum extract showed significantly different nocturnal locomotor activity and total sleep time compared to caffeine-treated Drosophila. Lotus seed and P. sibiricum extracts are attractive and valuable sleep-potentiating nutraceuticals. PMID:29333381

  14. Analysis of Flavonoids in Lotus (Nelumbo nucifera Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays

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    Ming-Zhi Zhu

    2015-06-01

    Full Text Available Lotus (Nelumbo nucifera leaves, a traditional Chinese medicinal herb, are rich in flavonoids. In an effort to thoroughly analyze their flavonoid components, macroporous resin chromatography coupled with HPLC-MS/MS was employed to simultaneously enrich and identify flavonoids from lotus leaves. Flavonoids extracted from lotus leaves were selectively enriched in the macroporous resin column, eluted subsequently as fraction II, and successively subjected to analysis with the HPLC-MS/MS and bioactivity assays. Altogether, fourteen flavonoids were identified, four of which were identified from lotus leaves for the first time, including quercetin 3-O-rhamnopyranosyl-(1→2-glucopyranoside, quercetin 3-O-arabinoside, diosmetin 7-O-hexose, and isorhamnetin 3-O-arabino- pyranosyl-(1→2-glucopyranoside. Further bioactivity assays revealed that these flavonoids from lotus leaves possess strong antioxidant activity, and demonstrate very good potential to be explored as food supplements or even pharmaceutical products to improve human health.

  15. Quantitative Determination of Alkaloids in Lotus Flower (Flower Buds of Nelumbo nucifera and Their Melanogenesis Inhibitory Activity

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    Toshio Morikawa

    2016-07-01

    Full Text Available A quantitative analytical method for five aporphine alkaloids, nuciferine (1, nornuciferine (2, N-methylasimilobine (3, asimilobine (4, and pronuciferine (5, and five benzylisoquinoline alkaloids, armepavine (6, norarmepavine (7, N-methylcoclaurine (8, coclaurine (9, and norjuziphine (10, identified as the constituents responsible for the melanogenesis inhibitory activity of the extracts of lotus flowers (the flower buds of Nelumbo nucifera, has been developed using liquid chromatography-mass spectrometry. The optimum conditions for separation and detection of these 10 alkaloids were achieved on a πNAP column, a reversed-phase column with naphthylethyl group-bonded silica packing material, with CH3CN–0.2% aqueous acetic acid as the mobile phase and using mass spectrometry equipped with a positive-mode electrospray ionization source. According to the protocol established, distributions of these 10 alkaloids in the petal, receptacle, and stamen parts, which were separated from the whole flower, were examined. As expected, excellent correlations were observed between the total alkaloid content and melanogenesis inhibitory activity. Among the active alkaloids, nornuciferine (2 was found to give a carbamate salt (2′′ via formation of an unstable carbamic acid (2′ by absorption of carbon dioxide from the air.

  16. Mode of Action Analyses of Neferine, a Bisbenzylisoquinoline Alkaloid of Lotus (Nelumbo nucifera against Multidrug-Resistant Tumor Cells

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    Onat Kadioglu

    2017-05-01

    Full Text Available Neferine, a bisbenzylisoquinoline alkaloid isolated from the green seed embryos of Lotus (Nelumbo nucifera Gaertn, has been previously shown to have various anti-cancer effects. In the present study, we evaluated the effect of neferine in terms of P-glycoprotein (P-gp inhibition via in vitro cytotoxicity assays, R123 uptake assays in drug-resistant cancer cells, in silico molecular docking analysis on human P-gp and in silico absorption, distribution, metabolism, and excretion (ADME, quantitative structure activity relationships (QSAR and toxicity analyses. Lipinski rule of five were mainly considered for the ADME evaluation and the preset descriptors including number of hydrogen bond donor, acceptor, hERG IC50, logp, logD were considered for the QSAR analyses. Neferine revealed higher toxicity toward paclitaxel- and doxorubicin-resistant breast, lung or colon cancer cells, implying collateral sensitivity of these cells toward neferine. Increased R123 uptake was observed in a comparable manner to the control P-gp inhibitor, verapamil. Molecular docking analyses revealed that neferine still interacts with P-gp, even if R123 was pre-bound. Bioinformatical ADME and toxicity analyses revealed that neferine possesses the druggability parameters with no predicted toxicity. In conclusion, neferine may allocate the P-gp drug-binding pocket and prevent R123 binding in agreement with P-gp inhibition experiments, where neferine increased R123 uptake.

  17. Immature Seed Endosperm and Embryo Proteomics of the Lotus (Nelumbo Nucifera Gaertn.) by One-Dimensional Gel-Based Tandem Mass Spectrometry and a Comparison with the Mature Endosperm Proteome

    OpenAIRE

    Moro, Carlo; Fukao, Yoichiro; Shibato, Junko; Rakwal, Randeep; Agrawal, Ganesh; Shioda, Seiji; Kouzuma, Yoshiaki; Yonekura, Masami

    2015-01-01

    Lotus (Nelumbo nucifera Gaertn.) seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed proteins and a comparison between the mature and immature endosperm tissues across the seed’s development. One-dimensional gel electrophoresis (SDS-PAGE) linked with tandem mass spectrome...

  18. Analysis of Accumulating Ability of Heavy Metals in Lotus (Nelumbo nucifera) Improved by Ion Implantation

    International Nuclear Information System (INIS)

    Zhang Jianhua; Wang Naiyan; Zhang Fengshou

    2012-01-01

    Heavy metals have seriously contaminated soil and water, and done harm to public health. Academician WANG Naiyan proposed that ion-implantation technique should be exploited for environmental bioremediation by mutating and breeding plants or microbes. By implanting N + into Taikonglian No.1, we have selected and bred two lotus cultivars, Jingguang No.1 and Jingguang No.2. The present study aims at analyzing the feasibility that irradiation can be used for remediation of soil and water from heavy metals. Compared with parent Taikonglian No.1, the uptaking and accumulating ability of heavy metals in two mutated cultivars was obviously improved. So ion implantation technique can indeed be used in bioremediation of heavy metals in soil and water, but it is hard to select and breed a cultivar which can remedy the soil and water from all the heavy metals.

  19. Immature Seed Endosperm and Embryo Proteomics of the Lotus (Nelumbo NuciferaGaertn.) by One-Dimensional Gel-Based Tandem Mass Spectrometry and a Comparison with the Mature Endosperm Proteome.

    Science.gov (United States)

    Moro, Carlo F; Fukao, Yoichiro; Shibato, Junko; Rakwal, Randeep; Agrawal, Ganesh Kumar; Shioda, Seiji; Kouzuma, Yoshiaki; Yonekura, Masami

    2015-08-14

    Lotus ( Nelumbo nucifera Gaertn.) seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed proteins and a comparison between the mature and immature endosperm tissues across the seed's development. One-dimensional gel electrophoresis (SDS-PAGE) linked with tandem mass spectrometry provided a protein inventory of the immature endosperm (122 non-redundant proteins) and embryo (141 non-redundant proteins) tissues. Comparing with the previous mature endosperm dataset (66 non-redundant proteins), a total of 206 non-redundant proteins were identified across all three tissues of the lotus seed. Results revealed some significant differences in proteome composition between the three lotus seed tissues, most notably between the mature endosperm and its immature developmental stage shifting the proteins from nutrient production to nutrient storage.

  20. Cytotoxic, Antitumor and Immunomodulatory Effects of the Water-Soluble Polysaccharides from Lotus (Nelumbo nucifera Gaertn. Seeds

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    Yafeng Zheng

    2016-11-01

    Full Text Available Lotus is an edible and medicinal plant, and the extracts from its different parts exhibit various bioactivities. In the present study, the hot water–soluble polysaccharides from lotus seeds (LSPS were evaluated for their cancer cell cytotoxicity, immunomodulatory and antitumor activities. LSPS showed significant inhibitory effects on the mouse gastric cancer MFC cells, human liver cancer HuH-7 cells and mouse hepatocarcinoma H22 cells. The animal studies showed that LSPS inhibited tumor growth in H22 tumor-bearing mice with the highest inhibition rate of 45.36%, which is comparable to that induced by cyclophosphamide (30 mg/kg treatment (50.79%. The concentrations of white blood cells were significantly reduced in cyclophosphamide-treated groups (p < 0.01, while LSPS showed much fewer side effects according to the hematology analysis. LSPS improved the immune response in H22 tumor-bearing mice by enhancing the spleen and thymus indexes, and increasing the levels of serum cytokines including tumor necrosis factor-α and interleukin-2. Moreover, LSPS also showed in vivo antioxidant activity by increasing superoxide dismutase activity, thus reducing the malondialdehyde level in the liver tissue. These results suggested that LSPS can be used as an antitumor and immunomodulatory agent.

  1. Identification and comparison of anti-inflammatory ingredients from different organs of Lotus nelumbo by UPLC/Q-TOF and PCA coupled with a NF-κB reporter gene assay.

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    Mengge Zhou

    Full Text Available Lotus nelumbo (LN (Nelumbo nucifera Gaertn. is an aquatic crop that is widely distributed throughout Asia and India, and various parts of this plant are edible and medicinal. It is noteworthy that different organs of this plant are used in traditional herbal medicine or folk recipes to cure different diseases and to relieve their corresponding symptoms. The compounds that are contained in each organ, which are named based on their chemical compositions, have led to their respective usages. In this work, a strategy was used to identify the difference ingredients and screen for Nuclear-factor-kappaB (NF-κB inhibitors with anti-inflammatory ability in LN. Seventeen main difference ingredients were compared and identified from 64 samples of 4 different organs by ultra-performance liquid chromatography that was coupled with quadrupole/time of flight mass spectrometry (UPLC/Q-TOF-MS with principal component analysis (PCA. A luciferase reporter assay system combined with the UPLC/Q-TOF-MS information was applied to screen biologically active substances. Ten NF-κB inhibitors from Lotus plumule (LP extracts, most of which were isoquinoline alkaloids or flavone C-glycosides, were screened. Heat map results showed that eight of these compounds were abundant in the LP. In conclusion, the LP extracts were considered to have the best anti-inflammatory ability of the four LN organs, and the chemical material basis (CMB of this biological activity was successfully validated by multivariate statistical analysis and biological research methods.

  2. Immature Seed Endosperm and Embryo Proteomics of the Lotus (Nelumbo Nucifera Gaertn. by One-Dimensional Gel-Based Tandem Mass Spectrometry and a Comparison with the Mature Endosperm Proteome

    Directory of Open Access Journals (Sweden)

    Carlo F. Moro

    2015-08-01

    Full Text Available Lotus (Nelumbo nucifera Gaertn. seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed proteins and a comparison between the mature and immature endosperm tissues across the seed’s development. One-dimensional gel electrophoresis (SDS-PAGE linked with tandem mass spectrometry provided a protein inventory of the immature endosperm (122 non-redundant proteins and embryo (141 non-redundant proteins tissues. Comparing with the previous mature endosperm dataset (66 non-redundant proteins, a total of 206 non-redundant proteins were identified across all three tissues of the lotus seed. Results revealed some significant differences in proteome composition between the three lotus seed tissues, most notably between the mature endosperm and its immature developmental stage shifting the proteins from nutrient production to nutrient storage.

  3. Purification of Four Flavonoid Glycosides from Lotus (Nelumbo nucifera Gaertn) plumule by Macroporous Resin Combined with HSCCC.

    Science.gov (United States)

    Wu, Lei; Xiong, Wei; Hu, Ju-Wu; Gu, Zhen; Xu, Jian-Guo; Si, Chuan-Ling; Bae, Young-Soo; Xu, Gang

    2018-02-01

    High-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column was successfully applied to the isolation and purification of four flavonoid glycosides from the medicinal herb Lotus plumule (LP). A polar two-phase solvent system composed of ethyl acetate-n-butanol-water (1:2:3, v/v/v) was selected by high-performance liquid chromatography (HPLC) and run on a preparative scale where the lower aqueous phase was used as the mobile phase with a head-to-tail elution mode. Quercetin-3-O-β-D-glucopyranoside (15 mg), isorhamnetin-3-O-β-D-glucopyranoside (13 mg), apigenin 6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside (18 mg) and apigenin 6,8-di-C-β-D-glucopyranoside (48 mg) were obtained in a one-step HSCCC separation from 240 mg of the sample. The purity of each compound was over 95% as determined by HPLC. Chemical structures of the isolated compounds were identified by electrospray ionization mass spectrometry (ESI-MS-MS) and nuclear magnetic resonance (NMR) methods. Moreover, the four compounds were isolated from LP for the first time. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. Molecular Structure-Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method.

    Science.gov (United States)

    Tang, Xiaosheng; Tang, Ping; Liu, Liangliang

    2017-06-23

    Lotus leaf has gained growing popularity as an ingredient in herbal formulations due to its various activities. As main functional components of lotus leaf, the difference in structure of flavonoids affected their binding properties and activities. In this paper, the existence of 11 flavonoids in lotus leaf extract was confirmed by High Performance Liquid Chromatography (HPLC) analysis and 11 flavonoids showed various contents in lotus leaf. The interactions between lotus leaf extract and two kinds of serum albumins (human serum albumin (HSA) and bovine serum albumin (BSA)) were investigated by spectroscopic methods. Based on the fluorescence quenching, the interactions between these flavonoids and serum albumins were further checked in detail. The relationship between the molecular properties of flavonoids and their affinities for serum albumins were analyzed and compared. The hydroxylation on 3 and 3' position increased the affinities for serum albumins. Moreover, both of the methylation on 3' position of quercetin and the C₂=C₃ double bond of apigenin and quercetin decreased the affinities for HSA and BSA. The glycosylation lowered the affinities for HSA and BSA depending on the type of sugar moiety. It revealed that the hydrogen bond force played an important role in binding flavonoids to HSA and BSA.

  5. Lotus-Inspired Nanotechnology Applications

    Indian Academy of Sciences (India)

    user

    The water-repellent surface of lotus (Nelumbo nucifera) leaf and flower is due to nanosized wax papillae on the upper side of each epidermal cell. As a result, raindrops make a high contact angle with the papillae and roll off carrying dust and dirt particles, leaving the surface clean. This self-cleaning property of highly ...

  6. Management and uses of Nelumbo nucifera Gaertsn. in Thai wetlands

    DEFF Research Database (Denmark)

    La-ongsri, W.; Trisonthi, C.; Balslev, Henrik

    2008-01-01

    Management and use of Nelumbo nucifera Gaertn.-the lotus plant-was studied in 58 wetlands distributed throughout Thailand. Although traditionally harvested in extractive systems depending on natural wetlands, N. nucifera is now increasingly being managed. Two hundred eighty informants mentioned 20...... related to the abundance of wetlands and natural stand in those regions, and maybe also cultural differences...

  7. Synchronicity of thermogenic activity, alternative pathway respiratory flux, AOX protein content, and carbohydrates in receptacle tissues of sacred lotus during floral development.

    Science.gov (United States)

    Grant, Nicole M; Miller, Rebecca E; Watling, Jennifer R; Robinson, Sharon A

    2008-01-01

    The relationships between heat production, alternative oxidase (AOX) pathway flux, AOX protein, and carbohydrates during floral development in Nelumbo nucifera (Gaertn.) were investigated. Three distinct physiological phases were identified: pre-thermogenic, thermogenic, and post-thermogenic. The shift to thermogenic activity was associated with a rapid, 10-fold increase in AOX protein. Similarly, a rapid decrease in AOX protein occurred post-thermogenesis. This synchronicity between AOX protein and thermogenic activity contrasts with other thermogenic plants where AOX protein increases some days prior to heating. AOX protein in thermogenic receptacles was significantly higher than in post-thermogenic and leaf tissues. Stable oxygen isotope measurements confirmed that the increased respiratory flux supporting thermogenesis was largely via the AOX, with little or no contribution from the cytochrome oxidase pathway. During the thermogenic phase, no significant relationship was found between AOX protein content and either heating or AOX flux, suggesting that regulation is likely to be post-translational. Further, no evidence of substrate limitation was found; starch accumulated during the early stages of floral development, peaking in thermogenic receptacles, before declining by 89% in post-thermogenic receptacles. Whilst coarse regulation of AOX flux occurs via protein synthesis, the ability to thermoregulate probably involves precise regulation of AOX protein, most probably by effectors such as alpha-keto acids.

  8. Phytochemical Profile and Biological Activity of Nelumbo nucifera

    Directory of Open Access Journals (Sweden)

    Keshav Raj Paudel

    2015-01-01

    Full Text Available Nelumbo nucifera Gaertn. (Nymphaeaceae is a potential aquatic crop grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in various systems of medicine including folk medicines, Ayurveda, Chinese traditional medicine, and oriental medicine. Many chemical constituents have been isolated till the date. However, the bioactive constituents of lotus are mainly alkaloids and flavonoids. Traditionally, the whole plant of lotus was used as astringent, emollient, and diuretic. It was used in the treatment of diarrhea, tissue inflammation, and homeostasis. The rhizome extract was used as antidiabetic and anti-inflammatory properties due to the presence of asteroidal triterpenoid. Leaves were used as an effective drug for hematemesis, epistaxis, hemoptysis, hematuria, and metrorrhagia. Flowers were used to treat diarrhea, cholera, fever, and hyperdipsia. In traditional medicine practice, seeds are used in the treatment of tissue inflammation, cancer and skin diseases, leprosy, and poison antidote. Embryo of lotus seeds is used in traditional Chinese medicine as Lian Zi Xin, which primarily helps to overcome nervous disorders, insomnia, and cardiovascular diseases (hypertension and arrhythmia. Nutritional value of lotus is as important as pharmaceutical value. These days’ different parts of lotus have been consumed as functional foods. Thus, lotus can be regarded as a potential nutraceutical source.

  9. Phytochemical Profile and Biological Activity of Nelumbo nucifera.

    Science.gov (United States)

    Paudel, Keshav Raj; Panth, Nisha

    2015-01-01

    Nelumbo nucifera Gaertn. (Nymphaeaceae) is a potential aquatic crop grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in various systems of medicine including folk medicines, Ayurveda, Chinese traditional medicine, and oriental medicine. Many chemical constituents have been isolated till the date. However, the bioactive constituents of lotus are mainly alkaloids and flavonoids. Traditionally, the whole plant of lotus was used as astringent, emollient, and diuretic. It was used in the treatment of diarrhea, tissue inflammation, and homeostasis. The rhizome extract was used as antidiabetic and anti-inflammatory properties due to the presence of asteroidal triterpenoid. Leaves were used as an effective drug for hematemesis, epistaxis, hemoptysis, hematuria, and metrorrhagia. Flowers were used to treat diarrhea, cholera, fever, and hyperdipsia. In traditional medicine practice, seeds are used in the treatment of tissue inflammation, cancer and skin diseases, leprosy, and poison antidote. Embryo of lotus seeds is used in traditional Chinese medicine as Lian Zi Xin, which primarily helps to overcome nervous disorders, insomnia, and cardiovascular diseases (hypertension and arrhythmia). Nutritional value of lotus is as important as pharmaceutical value. These days' different parts of lotus have been consumed as functional foods. Thus, lotus can be regarded as a potential nutraceutical source.

  10. Sacred Space.

    Science.gov (United States)

    Adelstein, Pamela

    2018-01-01

    A space can be sacred, providing those who inhabit a particular space with sense of transcendence-being connected to something greater than oneself. The sacredness may be inherent in the space, as for a religious institution or a serene place outdoors. Alternatively, a space may be made sacred by the people within it and events that occur there. As medical providers, we have the opportunity to create sacred space in our examination rooms and with our patient interactions. This sacred space can be healing to our patients and can bring us providers opportunities for increased connection, joy, and gratitude in our daily work.

  11. Antiplatelet activity of white and pink Nelumbo nucifera Gaertn flowers

    Directory of Open Access Journals (Sweden)

    Brindha Durairaj

    2010-09-01

    Full Text Available Nelumbo nucifera Gaertn (Nelumbonaceae a plant used in Ayurvedic medicine (common name: lotus, is a perennial, large and rhizomatous aquatic herb most prevalent in South India. Preliminary phytochemical screening of both white and pink Nelumbo nucifera flowers revealed the presence of phytochemical constituents (flavonoids, alkaloids, phenols etc,. Hence, an attempt has been made to screen the effect of Nelumbo nucifera flowers (both types on platelet aggregation. The antiplatelet activity of hydroethanolic extract of both types of flowers was studied using platelet-rich plasma in different concentrations (100-500µg/ml. Both white and pink Nelumbo nucifera flower extracts showed dose-dependent effective antiplatelet activity with maximum activity at 500µg/ml concentration; prevention of platelet aggregation was 50% of that achieved with standard aspirin. Furthermore, the antiplatelet activity of white flowers was relatively high (pNelumbo nucifera Gaertn (Nelumbonaceae, planta utilizada na medicina Ayurvédica, é erva aquática rizomatosa grande, predominante no sul da Índia. A triagem fitoquímica preliminar das flores brancas e cor-de-rosa de Nelumbo nucifera revelou a presença de constituintes fitoquímicos (flavonoides, alcaloides, fenóis etc. Assim, tentou-se a triagem do efeito das flores de Nelumbo nucifera de ambos os tipos na agregação plaquetária. A atividade antiplaquetária dos extratos hidroetanólico de ambos os tipos de flores foi estudada, utilizando-se plasma rico em plaquetas em duas diferentes concentrações (100 - 500 µg/mL. Tanto os extratos das flores brancas quanto daquelas de cor-de-rosa mostraram atividade antiplaquetária dose-dependente, com o máximo na concentração de 500 µg/mL. A prevenção da agregação plaquetária foi 50% daquela alcançada com o padrão de ácido acetilsalicílico. Além disso, a atividade antiplaquetária das flores brancas foi, relativamente, alta (p<0,05; ANOVA

  12. Ectopic expression of NnPER1, a Nelumbo nucifera 1-cysteine peroxiredoxin antioxidant, enhances seed longevity and stress tolerance in Arabidopsis.

    Science.gov (United States)

    Chen, Hu-Hui; Chu, Pu; Zhou, Yu-Liang; Ding, Yu; Li, Yin; Liu, Jun; Jiang, Li-Wen; Huang, Shang-Zhi

    2016-11-01

    Seed longevity, the maintenance of viability during storage, is a major factor for conservation of genetic resources and biodiversity. Seed longevity is an important trait of agriculture crop and is impaired by reactive oxygen species (ROS) during seed desiccation, storage and germination (C. R. Biol., 331, 2008 and 796). Seeds possess a wide range of systems (protection, detoxification, repair) allowing them to survive during storage and to preserve a high germination ability. In many plants, 1-cys peroxiredoxin (1-Cys Prx, also named PER1) is a seed-specific antioxidant which eliminates ROS with cysteine residues. Here we identified and characterized a seed-specific PER1 protein from seeds of sacred lotus (Nelumbo nucifera Gaertn.). Purified NnPER1 protein protects DNA against the cleavage by ROS in the mixed-function oxidation system. The transcription and protein accumulation of NnPER1 increased during seed desiccation and imbibition and under abiotic stress treatment. Ectopic expression of NnPER1 in Arabidopsis enhanced the seed germination ability after controlled deterioration treatment (CDT), indicating that NnPER1 improves seed longevity of transgenic plants. Consistent with the function of NnPER1 on detoxifying ROS, we found that the level of ROS release and lipid peroxidation was strikingly lower in transgenic seeds compared to wild-type with or without CDT. Furthermore, transgenic Arabidopsis seeds ectopic-expressing NnPER1 displayed enhanced tolerance to high temperature and abscisic acid (ABA), indicating that NnPER1 may participate in the thermotolerance and ABA signaling pathway. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.

  13. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    Directory of Open Access Journals (Sweden)

    Rong-Jyh Lin

    2014-02-01

    Full Text Available Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae, commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant, liriodenine (1, lysicamine (2, (--anonaine (3, (--asimilobine (4, (--caaverine (5, (--N-methylasimilobine (6, (--nuciferine (7, (--nornuciferine (8, (--roemerine (9, 7-hydroxydehydronuciferine (10 and cepharadione B (11 were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis. However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.

  14. Pollen Viability, Pistil Receptivity, and Embryo Development in Hybridization of Nelumbo nucifera Gaertn

    Directory of Open Access Journals (Sweden)

    Yan-Li Wang

    2012-01-01

    Full Text Available Seed set is usually low and differs for different crosses of flower lotus (Nelumbo nucifera Gaertn.. The reasons remain unknown, and this has a negative impact on lotus breeding. To determine the causes, we carried out two crosses of flower lotus, that is, “Jinsenianhua” × “Qinhuaihuadeng” and “Qinhuaihuadeng” × “Jinsenianhua” and pollen viability, pistil receptivity, and embryo development were investigated. The pollen grains collected at 05:00-06:00 hrs had the highest viability, and the viabilities of “Jinsenianhua” and “Qinhuaihuadeng” were 20.6 and 15.7%, respectively. At 4 h after artificial pollination, the number of pollen grains germinating on each stigma reached a peak: 63.0 and 17.2 per stigma in “Jinsenianhua” × “Qinhuaihuadeng” and “Qinhuaihuadeng” × “Jinsenianhua”, respectively. At 1 d after artificial pollination, the percentages of normal embryos in the two crosses were 55.0 and 21.9%, respectively; however, at 11 d after pollination, the corresponding percentages were 20.8 and 11.2%. Seed sets of the two crosses were 17.9 and 8.0%, respectively. The results suggested that low pistil receptivity and embryo abortion caused low seed set in “Qinhuaihuadeng” × “Jinsenianhua”, whereas low fecundity of “Jinsenianhua” × “Qinhuaihuadeng” was mainly attributable to embryo abortion.

  15. A REVIEW ON NELUMBO NUCIFERA GAERTN

    Science.gov (United States)

    Mukherjee, Pulok K.; Balasubramanian, R.; Saha, Kakali; Saha, B.P; Pal, M.

    1996-01-01

    Nelumbo nucifera Gaertn (Family- Nymphaeaceae) is a well known plant in ancient medical sciences, Much research have been done on the leaves, flowers, seeds and rhizomes of the plant, Our present aim is to review all the work performed on the plant to get a clear idea to evaluate its various medicinal principles relating to phytochemical pharmacological microbiological and allied approaches. PMID:22556755

  16. Alkaloids of Nelumbo lutea (Wild.) pers. (Nymphaeaceae)

    Science.gov (United States)

    Zelenski, S G

    1977-11-01

    A phytochemical investigation of an alcoholic extract of the petioles of Nelumbo lutea resulted in the identification of the alkaloids N-methylasimilobine, anonaine, and roemerine. The alkaloids nuciferine, armepavine, N-nornuciferine, and N-norarmepavine, previously previously reported in the whole plant, were also identified.

  17. Chemical constituents from Nelumbo nucifera leaves and their anti-obesity effects.

    Science.gov (United States)

    Ahn, Jong Hoon; Kim, Eun Sil; Lee, Chul; Kim, Soonok; Cho, Soo-Hyun; Hwang, Bang Yeon; Lee, Mi Kyeong

    2013-06-15

    Nelumbo nucifera Gaertn. (Nymphaeaceae), commonly called lotus, is widely distributed throughout Eastern Asia. It has been used for food and medicine for a long time. A phytochemical investigation of N. nucifera leaves led to the isolation of 13 megastigmanes (1-13), including a new megastigmane, nelumnucifoside A (1), and a new eudesmane sesquiterpene, nelumnucifoside B (14), eight alkaloids (15-22), and 11 flavonoids (23-33). Their chemical structures were determined based on spectroscopic methods including 1D, 2D NMR and MS spectrometry. The relative and absolute stereochemistry of the compounds was determined by NOESY and CD spectrometry, respectively. Compounds 19 and 22 significantly inhibited pancreatic lipase, whereas compounds 15 and 16 showed a strong inhibitory effect on adipocyte differentiation. Therefore, the leaves of N. nucifera have potential as an anti-obesity agent by inhibiting pancreatic lipase and adipocyte differentiation. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Taxonomy Icon Data: Lotus japonicus [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Lotus japonicus Lotus japonicus Lotus_japonicus_L.png Lotus_japonicus_NL.png Lotus_japonicus_S.png Lotus_jap...onicus_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lotus+japonicus&t=L ...http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lotus+japonicus&t=NL http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lotus+jap...onicus&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lotus+japonicus&t=NS ...

  19. Chemical changes associated with lotus and water lily natto production

    Science.gov (United States)

    Lestari, S. D.; Fatimah, N.; Nopianti, R.

    2017-04-01

    Natto is a traditional Japanese food made by fermenting whole soybean seeds with pure culture of Bacillus subtilis subsp. natto. The purpose of this study was to investigate the suitability of lotus (Nelumbo nucifera) and water lily (Nymphaea stellata) seeds as the raw materials for natto production. Chemical (proximate, amino acids and minerals) changes were observed on raw, steamed and fermented seeds. Proximate compositions of all samples were calculated in both wet basis and dry basis. In wet basis calculation, steaming and fermentation tended to lower the carbohydrates, ashes, fats and protein content which were attributed to the increase of moisture. The total amino acid, iron and magnesium contents of raw lotus seeds were 24.29%, 5.08 mg 100g-1 and 174.23 mg 100g-1 dry matter, respectively. After a 24h-fermentation at 40°C, the total amino acids decreased while iron and magnesium contents increased significantly reaching, in respective order, 9.9 mg 100g-1 and 411.36 mg 100g-1 dry matter. Changes in chemical composition after fermentation were more pronounced in lotus seeds than water lily seeds indicating that their nutrient composition were more suitable to support Bacillus subtilis growth.

  20. Sacred Way (Greek World)

    NARCIS (Netherlands)

    Williamson, Christina

    2015-01-01

    Sacred ways were roads that led to major sanctuaries, typically those located at a distance from the urban center, and were the vehicles for the processions involved in civic festivals at these shrines.

  1. Sacred Sky and Cyberspace

    Science.gov (United States)

    Clynes, F.

    2011-06-01

    The concept of the sacred world beyond the stars found expression in the works of Plato, into Gnosticism and was incorporated into Christianity where medieval images of the cosmos pictured the heavenly domain as beyond the stars. Today cyberspace literature abounds with descriptions of a transmundane space, a great Beyond. This talk looks at current views of cyberspace and asks if they are a re-packaging of the age-old concept of a sacred sky in a secular and technological format?

  2. Durable Lotus-effect surfaces with hierarchical structure using micro- and nanosized hydrophobic silica particles.

    Science.gov (United States)

    Ebert, Daniel; Bhushan, Bharat

    2012-02-15

    Surfaces with a very high apparent water contact angle (CA) and low water contact angle hysteresis (CAH) exhibit many useful characteristics, among them extreme water repellency, low drag for fluid flow, and a self-cleaning effect. The leaf of the Lotus plant (Nelumbo nucifera) achieves these properties using a hierarchical structure with roughness on both the micro- and nanoscale. It is of great interest to create durable surfaces with the so-called "Lotus effect" for many important applications. In this study, hierarchically structured surfaces with Lotus-effect properties were fabricated using micro- and nanosized hydrophobic silica particles and a simple spray method. In addition, hierarchically structured surfaces were prepared by spraying a nanoparticulate coating over a micropatterned surface. To examine the similarities between surfaces using microparticles versus a uniform micropattern as the microstructure, CA and CAH were compared across a range of pitch values for the two types of microstructures. Wear experiments were performed using an atomic force microscope (AFM), a ball-on-flat tribometer, and a water jet apparatus to verify multiscale wear resistance. These surfaces have potential uses in engineering applications requiring Lotus-effect properties and high durability. Copyright © 2011 Elsevier Inc. All rights reserved.

  3. Sustainability and sacred values

    Directory of Open Access Journals (Sweden)

    John Cairns Jr.

    2002-09-01

    Full Text Available Successful implementation of the quest for sustainable use of the planet requires that human society both reexamine and expand present views of what is sacred and what is not. The most important aspect will be going beyond a homocentric focus to a biocentric emphasis. A unifying theme would be the desire to leave a habitable planet for human descendants and those of other species. It is unlikely that society can be confident of achieving sustainability until persuasive evidence supporting this belief has existed for several generations. In order for sustainable use of the planet to persist indefinitely, the conditions essential to this state must be morally preserved on sacred grounds. Viewing natural systems as sacred requires not only preventing damage to them but, wherever possible, repairing damage to them caused by humankind.

  4. A general ionic liquid pH-zone-refining countercurrent chromatography method for separation of alkaloids from Nelumbo nucifera Gaertn.

    Science.gov (United States)

    Fang, Yingtong; Li, Quan; Shao, Qian; Wang, Binghai; Wei, Yun

    2017-07-21

    The alkaloids from lotus (Nelumbo nucifera Gaertn) are effective in lowering hyperlipemia and level of cholesterol. However, there is not a general method for their separation. In this work, a general ionic liquid pH-zone-refining countercurrent chromatography method for isolation and purification of six alkaloids from the whole lotus plant was successfully established by using ionic liquids as the modifier of the two-phase solvent system. The conditions of ionic liquid pH-zone-refining countercurrent chromatography, involving solvent systems, concentration of retainer and eluter, types of ionic liquids, the content of ionic liquids as well as ionic liquids posttreatment, were optimized to improve extraction efficiency. Finally, the separation of these six alkaloids was performed with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water-[C 4 mim][PF 6 ] at a volume ratio of 5:2:2:8:0.1, where 10mM TEA was added to the organic stationary phase as a retainer and 3mM HCl was added to the aqueous mobile phase as an eluter. As a result, six alkaloids including N-nornuciferine, liensinine, nuciferine, isoliensinine, roemerine and neferine were successfully separated with the purities of 97.0%, 90.2%, 94.7%, 92.8%, 90.4% and 95.9%, respectively. The established general method has been respectively applied to the crude samples of lotus leaves and lotus plumules. A total of 37.3mg of liensinine, 57.7mg of isoliensinine and 179.9mg of neferine were successfully purified in one run from 1.00g crude extract of lotus plumule with the purities of 93.2%, 96.5% and 98.8%, respectively. Amount of 45.6mg N-nornuciferine, 21.6mg nuciferine and 11.7mg roemerine was obtained in one step separation from 1.05g crude extract of lotus leaves with the purity of 96.9%, 95.6% and 91.33%, respectively. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Extraction of lotus fibres from lotus stems under microwave irradiation

    OpenAIRE

    Cheng, Cheng; Guo, Ronghui; Lan, Jianwu; Jiang, Shouxiang

    2017-01-01

    An efficient technology for preparing lotus fibres under microwave irradiation was developed. The lotus fibres were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, X-ray diffraction and thermogravimetry. Lotus fibres prepared are a kind of hollow fibres which are composed of a superfine fibre and an external shell. The effect of the treatment time with hydrogen peroxide under microwave irradiation on components, whiteness, moisture regain, removal rate ...

  6. The semiotics of the sacred

    Directory of Open Access Journals (Sweden)

    Bernard LAMIZET

    2011-01-01

    Full Text Available What is called sacred is an identity, a practice or a social actor who escapes ordinary social practices. In the 18th century, Kant call transcendancy a non religious way of sublimying identities, independant from space and time. So, speaking about sacred and transcendancy is a way to define political matters by their contrary. It is why sacred is a reference for psychic sublimation in unconscious crises. It is why psychoanalysis allows us to understand these forms of sublimation. Sacred is questioned when political identities are contested, and it is why sacred is a more important reference in middle age times (christianism and cathedrals building or, today (islamic or other religious references. On the contrary, secularism, which means separation between political powers and religious identities, is a major way of taking distance from sacred matters.

  7. Is the lotus leaf superhydrophobic?

    Science.gov (United States)

    Cheng, Yang-Tse; Rodak, Daniel E.

    2005-04-01

    Superhydrophobic surfaces have important technical applications ranging from self-cleaning window glasses, paints, and fabrics to low-friction surfaces. The archetype superhydrophobic surface is that of the lotus leaf. When rain falls on lotus leaves, water beads up with a contact angle in the superhydrophobic range of about 160°. The water drops promptly roll off the leaves collecting dirt along the way. This lotus effect has, in recent years, stimulated much research effort worldwide in the fabrication of surfaces with superhydrophobicity. But, is the lotus surface truly superhydrophobic? This work shows that the lotus leaves can be either hydrophobic or hydrophilic, depending on how the water gets on to their surfaces. This finding has significant ramifications on how to make and use superhydrophobic surfaces.

  8. Darwin's Sacred Cause

    DEFF Research Database (Denmark)

    Kjærgaard, Peter C.

    2009-01-01

    As we are being flooded by Darwin lollipops, t-shirts, quills and stamps it is becoming increasingly difficult to be heard or seen in the commercialised celebration in 2009. Some are in the business for the science, but a lot are in it for profit. Accordingly, the Darwin industry has left the hands...... of scholarly specialists and been appropriated by money makers. One could not help thinking about this as, in the autumn of 2008, the publisher began hyping Darwin's Sacred Cause as ‘one of the major contributions to the worldwide Darwin anniversary celebrations in 2009' Udgivelsesdato: February...

  9. Taxonomy Icon Data: Lotus corniculatus [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available L.png Lotus_corniculatus_S.png Lotus_corniculatus_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lo...tus+corniculatus&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lotus+corniculatus&t=NL http://biosciencedbc.jp/taxonom...y_icon/icon.cgi?i=Lotus+corniculatus&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Lotus+corniculatus&t=NS ...

  10. Lotus Base

    DEFF Research Database (Denmark)

    Mun, Terry; Bachmann, Asger; Gupta, Vikas

    2016-01-01

    Lotus japonicus is a well-characterized model legume widely used in the study of plant-microbe interactions. However, datasets from various Lotus studies are poorly integrated and lack interoperability. We recognize the need for a comprehensive repository that allows comprehensive and dynamic......,000 lines, and serves the end-user tightly integrated data from Lotus, such as the reference genome, annotated proteins, and expression profiling data. We report the integration of expression data from the L. japonicus gene expression atlas project, and the development of tools to cluster and export...

  11. Cloning and expression of an APETALA1-like gene from Nelumbo nucifera.

    Science.gov (United States)

    Kong, D Z; Shen, X Y; Guo, B; Dong, J X; Li, Y H; Liu, Y P

    2015-06-18

    The objective of this study was to clone the full-length cDNA of the APETALA1 (AP1) gene from lotus and analyze its sequence and expression pattern. The full-length cDNA sequence of the NnAP1 gene was amplified from the petals of Nelumbo nucifera 'Hongxia' using RT-PCR and rapid amplification of cDNA ends. Bioinformatic methods were used to analyze the sequence characteristics of the gene. Quantitative real-time PCR methods were used to investigate the expression pattern of NnAP1 in various organs and during different developmental stages. The cloned full-length NnAP1 cDNA (GenBank accession No. KF361315) was 902 bp, containing a 795-bp open reading frame encoding 264 amino acids with a relative molecular mass of 30,288.4 and an isoelectric point of 9.13. NnAP1 had a MADS-box domain and a K-box domain, which is typical of the SQUA/AP1 gene family. A protein sequence identity search showed that NnAP1 was 75-96% similar to other plant AP1s. Phylogenetic tree analysis indicated that NnAP1 was very closely related to AP1 of Glycine max, suggesting that they shared the same protein ancestor. Quantitative real-time PCR analysis showed that NnAP1 was expressed in various organs during different developmental stages; it had the highest expression in blooming flowers and had trace expression in the young vegetative and flower senescence stages. Our analysis suggests that NnAP1 plays an important role in controlling floral meristem identity and floral organ formation.

  12. Development of biological functional material and product from Nelumbo nucifera

    International Nuclear Information System (INIS)

    Jeong, Il Yun; Park, Yong Dae; Jin, Chang Hyun; Choi, Dae Seong

    2008-01-01

    The solvent extracts of Nelumbo nucifera G. were investigated for the activities of antioxidant, whitening, anti-wrinkle and antimicrobial effects to apply as a functional ingredient for cosmetic products. The electron donating ability of irradiated NN-L extract was above 85% at the concentration of 50ppm. The superoxide dismutase(SOD)-like activity of irradiated NN-L extract was about 76% at 1,000ppm concentration. The xanthine oxidase inhibitory effect of irradiated NN-L extract was about 15% at 1,000ppm. The tyrosinase inhibitory effect of irradiated NN-L extract was about 18% at 1,000ppm. Anti-wrinkle effect, the elastase inhibition activity of irradiated NN-L extract was about 45% at 1,000ppm concentration. All these findings suggested that Nelumbo nucifera G. has a great potential as a cosmeceutical ingredient

  13. Development of biological functional material and product from Nelumbo nucifera

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Il Yun; Park, Yong Dae; Jin, Chang Hyun; Choi, Dae Seong

    2008-01-15

    The solvent extracts of Nelumbo nucifera G. were investigated for the activities of antioxidant, whitening, anti-wrinkle and antimicrobial effects to apply as a functional ingredient for cosmetic products. The electron donating ability of irradiated NN-L extract was above 85% at the concentration of 50ppm. The superoxide dismutase(SOD)-like activity of irradiated NN-L extract was about 76% at 1,000ppm concentration. The xanthine oxidase inhibitory effect of irradiated NN-L extract was about 15% at 1,000ppm. The tyrosinase inhibitory effect of irradiated NN-L extract was about 18% at 1,000ppm. Anti-wrinkle effect, the elastase inhibition activity of irradiated NN-L extract was about 45% at 1,000ppm concentration. All these findings suggested that Nelumbo nucifera G. has a great potential as a cosmeceutical ingredient.

  14. PHARMACOGNOSTICAL PROFILE OF RHIZOME OF NELUMBO NUCIFERA GAERTN

    Science.gov (United States)

    Mukherjee, Pulok Kumar; Pal, M.; Pal, Saroj K.; Saha, Kakali; Saha, B.P.

    1998-01-01

    The rhizome of Nelumbo nucifera Gaertn (fam. Nymphaeaceae) was studied to fix the parameters for pharmacognostical standards. The present investigation deals with the macro and microscopically characters of rhizome along with studies on some physical constants, behavior of powdered rhizomes on treatment with different chemical reagents and florescence characteristics on exposure to U.V light, which would help in identification of the drug. PMID:22556855

  15. Development of lotus root fermented sugar syrup as a functional food supplement/condiment and evaluation of its physicochemical, nutritional and microbiological properties.

    Science.gov (United States)

    Shukla, Shruti; Park, Juyeon; Park, Jung Hyun; Lee, Jong Suk; Kim, Myunghee

    2018-02-01

    Lotus ( Nelumbo nucifera ) root has been used as an edible vegetable in East Asia for thousands of years. The present research was aimed to explore the physicochemical, nutritional and microbiological safety of lotus root fermented sugar syrup as a fermented food supplement or condiment for human health benefits. In this study, the physicochemical, nutritional and microbiological safety properties of lotus root syrup fermented with 57° Brix brown sugar at different time periods until 6 months (180 days) was investigated. There was a significant improvement as compared to 57° Brix brown sugar broth (as a control) in the total acceptability and physicochemical properties of lotus root sugar syrup samples such as pH and color improvement. The red color values of 180 days lotus root fermented sugar syrup samples were significantly enhanced (6.85 ± 0.58) when compared with the control (0.20 ± 0.15). In addition, the total protein content was increased from 8.27 ± 0.86 to 392.33 ± 7.19 μg/mL, along with the increase in fermentation time reaching to the level of consumption acceptability. All the lotus root fermented sugar syrup samples were subjected to microbiological analysis. It was found that the coliform, Bacillus cereus , Escherichia coli , Salmonella and Staphylococcus aureus counts were not detected in majority of the samples, confirming the high degree of hygiene processing of lotus root fermented sugar syrup samples for its use as a food supplement or condiment.

  16. Isolation and identification of a tribenzylisoquinoline alkaloid from Nelumbo nucifera Gaertn, a novel potential smooth muscle relaxant.

    Science.gov (United States)

    Yang, Guang-Ming; Sun, Jie; Pan, Yang; Zhang, Jun-Li; Xiao, Min; Zhu, Min-Sheng

    2018-01-01

    A new skeleton benzylisoquinoline (BI) named neoliensinine (1) was isolated from embryos of lotus seed (Nelumbo nucifera Gaertn.), a traditional Chinese herb. The tribenzylisoquinoline (TBI) structure of 1 was confirmed by interpreting spectroscopic data of UV, IR, MS, 1D and 2D NMR. The stereo-configurations of the new compound, together with two known bisbenzylisoquinolines (BBI), neferine and isoliensinine were established by analyzing 1 H NMR and 13 C NMR spectra. The relaxation of 1, neferine, isoliensinine and liensinine in isolated mesenteric vascular smooth muscle (VSM) was evaluated. All the four BIs could efficiently inhibit MVSM contraction induced by 124mM KCl, with IC 50 values of 2.407μM (1), 1.169μM (neferine), 3.504μM (isoliensinine) and 3.583μM (liensinine), respectively, suggesting that they were all potential relaxants for abnormal smooth muscle contractions. Interestingly, VSM treated by the three BBIs could re-contract when being stimulated by KCl after the drugs were removed, while VSM dealt with the TBI couldn't. It indicated that 1 has much high affinity with the molecular targets on relaxation of VSM contraction, which may relate to the unique skeleton with three BI groups. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Lotus and rhizobia – match and mismatch

    DEFF Research Database (Denmark)

    Albrektsen, Anita Søndergaard; Radutoiu, Elena Simona; Jurkiewicz, Anna

    Two Lotus species, L. japonicus and L. pedunculatus (uliginosus), show opposite nodule phenotypes when inoculated with rhizobial strains Mesorhizobium loti NZP2235 and Bradyrhizobium NZP2309. NZP2235 is inducing pink, nitrogen fixing nodules, when applied to Lotus japonicus, while Lotus peduncula...

  18. Changes in physicochemical properties related to the texture of lotus rhizomes subjected to heat blanching and calcium immersion.

    Science.gov (United States)

    Zhao, Wenlin; Xie, Wei; Du, Shenglan; Yan, Shoulei; Li, Jie; Wang, Qingzhang

    2016-11-15

    Pretreatments such as low temperature blanching and/or calcium soaking affect the cooked texture of vegetal food. In the work, lotus rhizomes (Nelumbo nucifera Gaertn.) were pretreated using the following 4 treatments, blanching at 40°C, blanching at 90°C, soaking in 0.5% CaCl2, and blanching at 40°C followed by immersion in 0.5% CaCl2. Subsequently, the cell wall material of pretreated samples was isolated and fractioned to identify changes in the degree of esterification (DE) and monosaccharide content of each section, and the texture of the lotus rhizomes in different pre-treatments was determined after thermal processing with different time. The results showed that the greatest hardness was obtained after blanching at 40°C in CaCl2, possibly attributing to the formation of a pectate calcium network, which maintains the integrity of cell walls. Furthermore, the content of galactose, rhamnose and arabinose decreased due to the breakage of sugar backbones and subsequent damage to cell walls. Our results may provide a reference for lotus rhizome processing. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Sacred places in global big cities

    DEFF Research Database (Denmark)

    Greve, Anni

    the relation between the sacred and the profane, and about the role of the sacred in modern societies. The question is if the development of modern societies has implied the gradual abolishment of religious beliefs, rituals and rites, or has it on the contrary implied the installment of new forms of religious...... for analyzing sacred places in big global cities. The leading motive of the paper is to present two cases of sacred places in global cities....

  20. Fannish tattooing and sacred identity

    Directory of Open Access Journals (Sweden)

    Bethan Jones

    2015-03-01

    Full Text Available Pleasure is an important motivation for fans to adopt texts. Fannish tattoos function to demonstrate affective investments in a text; they are also a performance of fandom and an example of sacred fan identity. Like engaging in cosplay or wearing clothing that features logos, fannish tattoos mark people as fans of a text. Furthermore, the more obscure the logo or fannish reference, the more performative the tattoo. Fannish tattoos help to construct a sacred fan identity. The sacred experience (as theorized by Émile Durkheim and his concept of the totem is imbued with meaning through choices that set it aside from the mundane. Within the context of fannish tattoos, fan affect gains similar significance.

  1. Lotus-Inspired Nanotechnology Applications

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 13; Issue 12. Lotus-Inspired Nanotechnology Applications. B Karthick Ramesh Maheshwari. General Article Volume 13 Issue 12 December 2008 pp 1141-1145. Fulltext. Click here to view fulltext PDF. Permanent link:

  2. Lotus-Inspired Nanotechnology Applications

    Indian Academy of Sciences (India)

    user

    . Wax is comprised of a mixture of long-chain hydrocarbons: B Karthick is a research ... flies) which cannot clean their flying structures by legs. In this case lotus effect works not only for the removal of particles, but also for maintenance of flight ...

  3. African Journal of Biotechnology - Vol 11, No 57 (2012)

    African Journals Online (AJOL)

    Recovery of antioxidant gene expression in sacred lotus (Nelumbo nucifera Gaertn.) embryonic axes enhances tolerance to extreme high temperature · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Yin Li, Yishun Zhang, Shaowei Wei, Li Liu, Yunfeng Chen, 12011- ...

  4. Antiulcerogenic activity of Zizyphus lotus (L.) extracts.

    Science.gov (United States)

    Wahida, Borgi; Abderrahman, Bouraoui; Nabil, Chouchane

    2007-06-13

    Oral administration of aqueous extracts of Zizyphus lotus root barks (50-200 mg/kg) leaves (50-200 mg/kg) and fruits (200-400 mg/kg) produced a significant (pZizyphus lotus extracts act essentially as cytoprotective agents, which support the antiulcer effect of this plant in the traditional medicine.

  5. Legume and Lotus japonicus Databases

    DEFF Research Database (Denmark)

    Hirakawa, Hideki; Mun, Terry; Sato, Shusei

    2014-01-01

    Since the genome sequence of Lotus japonicus, a model plant of family Fabaceae, was determined in 2008 (Sato et al. 2008), the genomes of other members of the Fabaceae family, soybean (Glycine max) (Schmutz et al. 2010) and Medicago truncatula (Young et al. 2011), have been sequenced. In this sec....... In this section, we introduce representative, publicly accessible online resources related to plant materials, integrated databases containing legume genome information, and databases for genome sequence and derived marker information of legume species including L. japonicus...

  6. Sacred Groves, Spirituality and Sustainable Development in ...

    African Journals Online (AJOL)

    Since creation, mankind has strived to maintain a positive relationship with nature by preserving and making certain specific trees, water bodies, highlands and other places sacred. The practice of keeping sacred groves is one of the ways which promotes this human, ecological and spiritual connection. These groves ...

  7. Sacred Space and Sublime Sacramental Piety

    DEFF Research Database (Denmark)

    Petersen, Nils Holger

    2012-01-01

    Analyses and Discussions of Mozart's Sacramental Litanies K. 125 and K. 243 in relation to the notions of the Sacred and the Sublime.......Analyses and Discussions of Mozart's Sacramental Litanies K. 125 and K. 243 in relation to the notions of the Sacred and the Sublime....

  8. Le sacré sauvage

    Directory of Open Access Journals (Sweden)

    Roger Bastide

    2010-09-01

    Full Text Available Conférence prononcée le 13 septembre 1973 dans le cadre des Rencontres Internationales de Genève qui avaient pour thème cette année-là « Le besoin religieux ». Cette conférence figure dans les Actes de cette rencontre (Le Besoin religieux, Éditions de la Baconnière, Neuchâtel, 1974, pp. 123-145. Elle a été republiée dans un ouvrage posthume dont elle est l’éponyme, ouvrage édité par Henri Desroche (Paris, éditions Stock, 1975. Nous reproduisons ici le texte intégral original, avec l’aimable autorisation des Rencontres Internationales de Genève auxquelles SociologieS adresse ses remerciements.The wild sacredText of a lecture taken on November the 13th 1973 during the Rencontres Internationales de Genève whom theme was "The religious need". This lecture was first published in: Le Besoin religieux (La Baconnière Editions, Neuchâtel, 1974, pp. 123-145, and published once again in a posthumous book: Le sacré sauvage ("The wild sacred" edited by Henri Desroche (Paris, Stock Editions, 1975. It is reproduced here with the kind authorization of the Rencontres Internationales de Genève. SociologieS gives them sincere thanks.Le Sacré sauvage (Lo sagrado en el mundo primitivoConferencia efectuada el 13 de septiembre de 1973 en el marco de las Rencontres Internationales de Genève (Encuentros Internacionales de Ginebra que tenia como tema general la necesidad de la experiencia religiosa (“Le besoin religieux”, Éditions de la Baconnière, Neuchâtel, 1974, pp. 123-145. Esta conferencia fue publicada de nuevo en un trabajo póstumo que lleva el mismo título, editado por Henri Desroche (Paris, Éditions Stock, 1975. Reproducimos aquí la integralidad del texto original con la amable autorización de las Rencontres Internationales de Genève, a las cuales la revista SociologieS expresa su agradecimiento.

  9. The Truth of Sacred Scripture

    Directory of Open Access Journals (Sweden)

    Tomasz Jelonek

    2006-09-01

    Full Text Available Article presents the history of contradiction between science and the Bible and how it was solved in Dogmatic Constitution on Divine Revelation Dei Verbum of the II Vatican Council. Since biblical truth was given to us “for the sake of our salvation,” and not in order to teach us natural science or history for their own sake, Sacred Scripture cannot be fairly judged to be in error when it sometimes presents historical or scientific truth in a less complete, less detailed, more popular, or more imprecise (i.e. merely approximate fashion than would be acceptable in modern texts dedicated formally to those disciplines.

  10. Lotus Base: An integrated information portal for the model legume Lotus japonicus.

    Science.gov (United States)

    Mun, Terry; Bachmann, Asger; Gupta, Vikas; Stougaard, Jens; Andersen, Stig U

    2016-12-23

    Lotus japonicus is a well-characterized model legume widely used in the study of plant-microbe interactions. However, datasets from various Lotus studies are poorly integrated and lack interoperability. We recognize the need for a comprehensive repository that allows comprehensive and dynamic exploration of Lotus genomic and transcriptomic data. Equally important are user-friendly in-browser tools designed for data visualization and interpretation. Here, we present Lotus Base, which opens to the research community a large, established LORE1 insertion mutant population containing an excess of 120,000 lines, and serves the end-user tightly integrated data from Lotus, such as the reference genome, annotated proteins, and expression profiling data. We report the integration of expression data from the L. japonicus gene expression atlas project, and the development of tools to cluster and export such data, allowing users to construct, visualize, and annotate co-expression gene networks. Lotus Base takes advantage of modern advances in browser technology to deliver powerful data interpretation for biologists. Its modular construction and publicly available application programming interface enable developers to tap into the wealth of integrated Lotus data. Lotus Base is freely accessible at: https://lotus.au.dk.

  11. Sacred space and the healing journey.

    Science.gov (United States)

    Alt, Paul L

    2017-07-01

    Sacred space and spirituality have long been used to heal the mind, body, and spirit. This article illuminates the origins of sacred space and its role as a healing environment from the first human construct, the burial mound, to the 5th Century BCE Greek healing city of Epidaurus. It then examines the role of spirituality as one of the necessary human institutions for a healthy society, according to the Italian philosopher Giambattista Vico. The conclusion then surveys three contemporary healing environments' architecture, the Department of Veteran Affairs Healing Environment Design Guideline (VAHEDG), and how these sacred spaces mend individual and community ailments.

  12. Nutritional composition of Zizyphus lotus L. seeds.

    Science.gov (United States)

    Chouaibi, Moncef; Mahfoudhi, Nesrine; Rezig, Leila; Donsì, Francesco; Ferrari, Giovanna; Hamdi, Salem

    2012-04-01

    Zizyphus lotus seeds are an unutilized source of vegetable oil and protein and nothing has been reported on their physicochemical characteristics which would indicate the potential uses of these seeds. The percentage composition of the Zizyphus lotus seeds is (on a dry-weight basis): ash 1.05%, oil 32.92%, protein 19.11%, total carbohydrate 40.87% and moisture 6.05%. Calcium, potassium and magnesium constitute the major minerals of Zizyphus lotus seeds. The seed proteins are rich in threonine, glutamic acid, leucine, arginine and aspartic acid (26.73%, 17.28%, 13.11%, 9.47% and 7.76%, respectively). The main fatty acids of the oil are oleic (61.93%), linoleic (18.31%) and palmitic (9.14%) acids. Glycerol trioleate (OOO; O: oleic acid) was the most abundant triacylglycerol, representing 26.48% of the total triacyglycerols. β-Tocopherol was the major tocopherol (130.47 mg 100 g(-1) ). This oil was rich in Δ7-campestrol and β-sitosterol (147.82 and 82.10 mg 100 g(-1) oil), respectively. Zizyphus lotus seeds are rich in fat and protein which are of potential industrial significance. In addition, Zizyphus lotus L. seed oil contained many bioactive compounds. This fact is of great economic interest owing to several applications of Zizyphus lotus L. seeds in the food, cosmetics and medicinal industries. Copyright © 2011 Society of Chemical Industry.

  13. Natural pigments and sacred art

    Science.gov (United States)

    Kelekian, Lena, ,, Lady

    2010-05-01

    Since the dawn of mankind, cavemen has expressed himself through art. The earliest known cave paintings date to some 32,000 years ago and used 4 colours derived from the earth. These pigments were iron oxides and known as ochres, blacks and whites. All pigments known by the Egyptians, the Greeks, the Romans and Renaissance man were natural and it was not until the 18th century that synthetic pigments were made and widely used. Until that time all art, be it sacred or secular used only natural pigments, of which the preparation of many have been lost or rarely used because of their tedious preparation. As a geologist, a mineralogist and an artist specializing in iconography, I have been able to rediscover 89 natural pigments extracted from minerals. I use these pigments to paint my icons in the traditional Byzantine manner and also to restore old icons, bringing back their glamour and conserving them for years to come. The use of the natural pigments in its proper way also helps to preserve the traditional skills of the iconographer. In the ancient past, pigments were extremely precious. Many took an exceedingly long journey to reach the artists, and came from remote countries. Research into these pigments is the work of history, geography and anthropology. It is an interesting journey in itself to discover that the blue aquamarines came from Afghanistan, the reds from Spain, the greens Africa, and so on. In this contribution I will be describing the origins, preparation and use of some natural pigments, together with their history and provenance. Additionally, I will show how the natural pigments are used in the creation of an icon. Being a geologist iconographer, for me, is a sacrement that transforms that which is earthly, material and natural into a thing of beauty that is sacred. As bread and wine in the Eucharist, water during baptism and oil in Holy Union transmit sanctification to the beholder, natural pigments do the same when one considers an icon. The

  14. Dammarane saponins from Zizyphus lotus.

    Science.gov (United States)

    Renault, J H; Ghedira, K; Thepenier, P; Lavaud, C; Zeches-Hanrot, M; Le Men-Olivier, L

    1997-04-01

    Four dammarane-type saponins were isolated by means of centrifugal partition chromatography from the root bark of Zizyphus lotus. Their structures were elucidated using a combination of 1D and 2D 1H and 13C NMR spectra and mass spectroscopy. One of these glycosides is the known jujuboside A. The others are three new dammarane saponins, identified as 3-O-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl (1-->3)-[alpha-L-rhamnopyranosyl(1-->2)]-alpha-L-arabinopyranosyl jujubogenin = jujuboside C, 3-O-alpha-L-rhamnopyranosyl (1-->2)-[beta-D-glucopyranosyl(1-->3)]-beta-D-galactopyranosyl lotogenin = lotoside I, and 3-O-alpha-L-rhamnopyranosyl(1-->2)-[beta-D-glucopyranosyl (1-->3)]-beta-D-glucopyranosyl lotogenin = lotoside II. Lotogenin is a new dammarane derivative identified as (15R, 16R, 20R, 22R)-16 beta, 22-epoxydammar-24-ene-3 beta, 15 alpha, 16 alpha, 20 beta-tetraol.

  15. The Lotus effect: superhydrophobicity and metastability.

    Science.gov (United States)

    Marmur, Abraham

    2004-04-27

    To learn how to mimic the Lotus effect, superhydrophobicity of a model system that resembles the Lotus leaf is theoretically discussed. Superhydrophobicity is defined by two criteria: a very high water contact angle and a very low roll-off angle. Since it is very difficult to calculate the latter for rough surfaces, it is proposed here to use the criterion of a very low wet (solid-liquid) contact area as a simple, approximate substitute for the roll-off angle criterion. It is concluded that nature employs metastable states in the heterogeneous wetting regime as the key to superhydrophobicity on Lotus leaves. This strategy results in two advantages: (a) it avoids the need for high steepness protrusions that may be sensitive to breakage and (b) it lowers the sensitivity of the superhydrophobic states to the protrusion distance.

  16. Contribution of sacred forests to biodiversity conservation: case of ...

    African Journals Online (AJOL)

    In the current context of the rapid changes of land cover and land use in Benin, sacred forests are thought to be sanctuaries of biodiversity and representative of primary vegetation. In order to assess the contribution of sacred forests to phytodiversity conservation, this study was carried out in Adjahouto and Lokozoun sacred ...

  17. Floristic Composition of Traditional Sacred Landscapes in Bedelle ...

    African Journals Online (AJOL)

    This study was conducted on the Floristic Composition of Sacred Landscapes in Bedelle Woreda, Illubabor Zone, Oromia Regional State. The aim of the study was to assess the plant species diversity in the sacred sites of Bedelle Woreda. Three sacred sites (Boqa in Banshure Kebele, Boqa in Socho Kebele and Mute in ...

  18. Morphometric characteristics of Lotus corniculatus L. genotypes ...

    African Journals Online (AJOL)

    The aim of this study was to examine the degree of variability in morphological and agronomic characteristics of 20 Lotus corniculatus L. local genotypes, and also to set aside germplasm that will be used as a source of genetic basis for improvement of the studied properties. In poor quality soils, L. corniculatus L. plays an ...

  19. Phytophilous Macroinvertebrates of Floating Nymphaea lotus and ...

    African Journals Online (AJOL)

    Phytophilous macroinvertebrates associated with two dominant floating aquatic macrophytes, water lilly (Nymphaea lotus) and water lettuce (Pistia stratiotes) in River Orogodo, Niger Delta were sampled between February and July 2006. The location of both plant species exhibited similar water quality characteristics during ...

  20. Rituals and Sacred Space of Pandharpur, India

    DEFF Research Database (Denmark)

    Sand, Erik

    2011-01-01

    The small town Pandharpur, situated about two hundred kilometres south east of Pune, is one of the most popular sacred places in the state of Maharashtra, India. It is dedicated to the god Vithoba who is considered to be an incarnation of Vishnu in the form of Krishna. Pandharpur and Vithoba plays...

  1. Antibacterial Characteristics of Lotus-Type Porous Copper

    OpenAIRE

    Lee, Jin-Soo; Lee, Yun-Soo; Kim, Mok-Soon; Hyun, Soong-Keun; Kang, Chang-Ho; So, Jae-Seong; Yoon, Eui-Han

    2013-01-01

    Lotus-type porous copper with long cylindrical pores aligned parallel to the solidification direction was studied for use as an antibacterial material. The antibacterial performance of lotus-type porous copper samples with different specific surface areas against Escherichia coli was investigated. The results confirmed that the antibacterial effect gradually increased with specific surface area. The correlation between the pore structure of lotus-type porous metals and their antibacterial eff...

  2. Texture and wettability of metallic lotus leaves

    Science.gov (United States)

    Frankiewicz, C.; Attinger, D.

    2016-02-01

    Superhydrophobic surfaces with the self-cleaning behavior of lotus leaves are sought for drag reduction and phase change heat transfer applications. These superrepellent surfaces have traditionally been fabricated by random or deterministic texturing of a hydrophobic material. Recently, superrepellent surfaces have also been made from hydrophilic materials, by deterministic texturing using photolithography, without low-surface energy coating. Here, we show that hydrophilic materials can also be made superrepellent to water by chemical texturing, a stochastic rather than deterministic process. These metallic surfaces are the first analog of lotus leaves, in terms of wettability, texture and repellency. A mechanistic model is also proposed to describe the influence of multiple tiers of roughness on wettability and repellency. This demonstrated ability to make hydrophilic materials superrepellent without deterministic structuring or additional coatings opens the way to large scale and robust manufacturing of superrepellent surfaces.Superhydrophobic surfaces with the self-cleaning behavior of lotus leaves are sought for drag reduction and phase change heat transfer applications. These superrepellent surfaces have traditionally been fabricated by random or deterministic texturing of a hydrophobic material. Recently, superrepellent surfaces have also been made from hydrophilic materials, by deterministic texturing using photolithography, without low-surface energy coating. Here, we show that hydrophilic materials can also be made superrepellent to water by chemical texturing, a stochastic rather than deterministic process. These metallic surfaces are the first analog of lotus leaves, in terms of wettability, texture and repellency. A mechanistic model is also proposed to describe the influence of multiple tiers of roughness on wettability and repellency. This demonstrated ability to make hydrophilic materials superrepellent without deterministic structuring or additional

  3. Profaning the Sacred in Leadership Studies

    DEFF Research Database (Denmark)

    Sliwa, Martyna; Spoelstra, Sverre; Meier Sørensen, Bent

    2013-01-01

    The leadership literature is full of stories of heroic self-sacrifice. Sacrificial leadership behaviour, some scholars conclude, is to be recommended. In this article we follow Keith Grint's conceptualization of leadership as necessarily pertaining to the sacred, but-drawing on Giorgio Agamben...... of the novel, we discuss how it contributes to profaning particular notions of sacrifice and the sacred in leadership thinking. In the novel, self-sacrifice does not function as a way of establishing a leadership position, but as a way to avoid the dangers associated with leadership, and possibly redeem humans......'s notion of profanation-we highlight the need for organization scholars to profane the sacralizations embedded in leadership thinking. One example of this, which guides us throughout the article, is the novel A Wild Sheep Chase, by the Japanese author Haruki Murakami. By means of a thematic reading...

  4. Meteorites as 'Sacred stones' from sky

    International Nuclear Information System (INIS)

    Oezmaya, S.

    2005-01-01

    Meteorites which are stony and iron remnants from early solar system are falling on the planetary surfaces including Earth, since the birt of Solar System, about 4.5 billion years ago. Several of the meteorites have also been used as sacred stones from gods, throughout the human history. Most famous contemporary example still having a sacred function is in the Kaaba, at Mecca. There are other examples of historical records where such stones are mentioned and sometimes, sacrificed. Artemis Temple of Ephesus is known to contain such a stone which is still not recovered. Dating of such celestial artifacts through modern techniques with examples from Turkey is expected to provide much useful information to historical as well as scientific ends

  5. Psychoanalysis: the sacred and the profane.

    Science.gov (United States)

    Frosch, Allan

    2014-06-01

    Colleagues from a variety of perspectives have written about the propensity to enshrine psychoanalytic theory. The meaning of the word "enshrine" is to cherish as sacred an idea or philosophy and protect it from change. In other words, the way we view psychoanalysis, our theories of mind and technique, become holy writ and we have divided the world of theory into the sacred and the profane. This is the kiss of death for theory, which must constantly evolve and change, but comforting for the analyst who believes he is on the side of the right, the sacred. In this paper I will discuss how our propensity to enshrine theory has had a debilitating effect on the development of psychoanalysis and, in particular, as a treatment for the most vulnerable people who seek our help. I also address the idea that movement away from enshrined positions allows us to construct different versions of reality. In this context, the notion of "action at a distance" is presented along with the attendant idea of psychoanalytic entanglement.

  6. Comparative larvicidal efficacies of Nymphae lotus fractions against ...

    African Journals Online (AJOL)

    Comparative larvicidal efficacies of Nymphae lotus fractions against Anopheles mosquito larvae. A.A. Yakubu, A.A. Salisu, D.B. Hassan, T.S. Imam. Abstract. No Abstract. Keywords: Larvicidal, Nymphaea lotus, Anopheles, leaves extracts, fractions, Culicidae. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  7. Effects of gamma irradiation on total polyphenols, radical scavenging activities and decolourization of Nelumbo nucifera extracts

    International Nuclear Information System (INIS)

    Jeong, Il Yun; Lee, Hyo Jung; Park, Yong Dae; Jin, Chang Hyun; Choi, Dae Seong; Byun, Myung Woo

    2009-01-01

    The ethanolic leaf extract of Nelumbo nucifera (NC) was exposed to γ-irradiation, and its antioxidant activities, total polyphenols and colour characteristics were studied to discern its potential ability as a food or cosmetic materials. The results demonstrated that the radical scavenging activities and total polyphenols of the γ-irradiated leaf extract of NC were not observed to be significantly different. However, γ-irradiation significantly increased the Hunter colour L*-value at doses of 20 and 50 kGy, while the Hunter colour b*-values were decreased under the same conditions.

  8. Effects of gamma irradiation on total polyphenols, radical scavenging activities and decolourization of Nelumbo nucifera extracts

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Il Yun [Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeong-up 580-185 (Korea, Republic of)], E-mail: iyjeong@kaeri.re.kr; Lee, Hyo Jung; Park, Yong Dae; Jin, Chang Hyun; Choi, Dae Seong; Byun, Myung Woo [Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeong-up 580-185 (Korea, Republic of)

    2009-07-15

    The ethanolic leaf extract of Nelumbo nucifera (NC) was exposed to {gamma}-irradiation, and its antioxidant activities, total polyphenols and colour characteristics were studied to discern its potential ability as a food or cosmetic materials. The results demonstrated that the radical scavenging activities and total polyphenols of the {gamma}-irradiated leaf extract of NC were not observed to be significantly different. However, {gamma}-irradiation significantly increased the Hunter colour L*-value at doses of 20 and 50 kGy, while the Hunter colour b*-values were decreased under the same conditions.

  9. Sub-µ structured Lotus Surfaces Manufacturing

    DEFF Research Database (Denmark)

    Worgull, Matthias; Heckele, Mathias; Mappes, Timo

    2008-01-01

    Sub-micro structured surfaces allow modifying the behavior of polymer films or components. Especially in micro fluidics a lotus-like characteristic is requested for many applications. Structure details with a high aspect ratio are necessary to decouple the bottom and the top of the functional layer....... Unlike to stochastic methods, patternin¬g with a LIGA-mold insert it is possible to structure surfaces very uniformly or even with controlled variations (e.g. with gradients). In this paper we present the process chain to realize polymer sub-micro structures with minimum lateral feature size of 400 nm...

  10. Sacred Forests : Indigenous Knowledge and Cultural Beliefs for ...

    African Journals Online (AJOL)

    There should be policies that protect the indigenous peoples' cultural heritage such as the sacred forest so that they would not be tampered with during developmental projects resulting from urbanisation and other land competing activities. Keywords: Sacred forest, cultural belief, conservation, indigenous knowledge, ...

  11. Socio- cultural importance of sacred forests conservation in south ...

    African Journals Online (AJOL)

    African Journal of Sustainable Development ... This is an example of in-situ biodiversity conservation, which has supported various ecosystem functions. ... campaign targeting relevant stakeholders and on the potentials of sacred forest in-situ conservation can go a long way in strengthening sacred forest sustainability.

  12. Floristic Composition of Traditional Sacred Landscapes in Bedelle ...

    African Journals Online (AJOL)

    2009-12-21

    Dec 21, 2009 ... This study was conducted on the Floristic Composition of Sacred Landscapes in Bedelle. Woreda, Illubabor .... Data analysis. In the data analysis, excel spread sheet and. Biodiversity Professional Version 2 were used. Species diversity, richness and evenness in the sacred sites, and the association of the ...

  13. Eboo Patel (2012). Sacred Ground: Pluralism, Prejudice and the ...

    African Journals Online (AJOL)

    Sacred Ground: Pluralism, Prejudice and the Promise of America. Boston: Beacon Press. Reviewed by Jon C. Dalton*. In his 2012 book, Sacred Ground, Eboo Patel, an American Muslim and President of the Interfaith Student Core in Chicago, Illinois, analyses the sources of contemporary interreligious conflict and offers ...

  14. Original Paper Contribution of sacred forests to biodiversity ...

    African Journals Online (AJOL)

    In the current context of the rapid changes of land cover and land use in Benin, sacred forests are thought to be sanctuaries of biodiversity and representative of primary vegetation. In order to assess the contribution of sacred forests to phytodiversity conservation, this study was carried out in Adjahouto and. Lokozoun ...

  15. Veiled Word(s) – Sacred Silence

    DEFF Research Database (Denmark)

    Isar, Nicoletta

    2014-01-01

    This paper defines the Byzantine altar as a sacred space of participative performance in the mystery of the Eucharist – a paradigm of “timeless re-enactment of the Christian drama.” Reading a series of Byzantine ekphraseis, the paper addresses such concepts as inner vision (theoria), silent...... or secret prayer, and divine silence, which are at the very centre of the Byzantine altar. The main focus is to investigate the liminal nature of the Mystery, manifested through concealing-revealing devices, which are thresholds in the liturgical participation of the Byzantine subject. Fear and secrecy...

  16. Rituals and Sacred Space of Pandharpur, India

    DEFF Research Database (Denmark)

    Sand, Erik

    2011-01-01

    a very important role in the religious life of the so-called Varakari Sampradaya which goes back to at least the time of Jnadeva or Jnaneshvara in the second half of the 13th century. In fact the religious life of the town is to a great extent dominated by the presence of this Sampradaya, and the great...... completely dominated by the literature of the Varakari Sampradaya. In this paper I shall, however, be dealing with two Sanskrit mahatmyas, both called Pandurangamahatmya, and the ideas found therein with regard to the rituals and sacred space of Pandharpur....

  17. The sacred, the secular, and the profane

    DEFF Research Database (Denmark)

    Tsinovoi, Alexei

    2017-01-01

    In security studies, the dichotomy between ‘security’ and ‘regular’ politics has been effectively challenged in recent years, both theoretically and empirically. To address this challenge, the article develops Giorgio Agamben’s concepts of the sacred, the profane and the secular into three ideal...... it is different from security, and how their exclusionary practices can be contested effectively. The article demonstrates how Agamben’s concepts improve our understanding of contemporary configurations of security and politics, highlighting that what is at stake today in security analysis is not only...

  18. Superhydrophobicity in perfection: the outstanding properties of the lotus leaf.

    Science.gov (United States)

    Ensikat, Hans J; Ditsche-Kuru, Petra; Neinhuis, Christoph; Barthlott, Wilhelm

    2011-01-01

    Lotus leaves have become an icon for superhydrophobicity and self-cleaning surfaces, and have led to the concept of the 'Lotus effect'. Although many other plants have superhydrophobic surfaces with almost similar contact angles, the lotus shows better stability and perfection of its water repellency. Here, we compare the relevant properties such as the micro- and nano-structure, the chemical composition of the waxes and the mechanical properties of lotus with its competitors. It soon becomes obvious that the upper epidermis of the lotus leaf has developed some unrivaled optimizations. The extraordinary shape and the density of the papillae are the basis for the extremely reduced contact area between surface and water drops. The exceptional dense layer of very small epicuticular wax tubules is a result of their unique chemical composition. The mechanical robustness of the papillae and the wax tubules reduce damage and are the basis for the perfection and durability of the water repellency. A reason for the optimization, particularly of the upper side of the lotus leaf, can be deduced from the fact that the stomata are located in the upper epidermis. Here, the impact of rain and contamination is higher than on the lower epidermis. The lotus plant has successfully developed an excellent protection for this delicate epistomatic surface of its leaves.

  19. Superhydrophobicity in perfection: the outstanding properties of the lotus leaf

    Directory of Open Access Journals (Sweden)

    Hans J. Ensikat

    2011-03-01

    Full Text Available Lotus leaves have become an icon for superhydrophobicity and self-cleaning surfaces, and have led to the concept of the ‘Lotus effect’. Although many other plants have superhydrophobic surfaces with almost similar contact angles, the lotus shows better stability and perfection of its water repellency. Here, we compare the relevant properties such as the micro- and nano-structure, the chemical composition of the waxes and the mechanical properties of lotus with its competitors. It soon becomes obvious that the upper epidermis of the lotus leaf has developed some unrivaled optimizations. The extraordinary shape and the density of the papillae are the basis for the extremely reduced contact area between surface and water drops. The exceptional dense layer of very small epicuticular wax tubules is a result of their unique chemical composition. The mechanical robustness of the papillae and the wax tubules reduce damage and are the basis for the perfection and durability of the water repellency. A reason for the optimization, particularly of the upper side of the lotus leaf, can be deduced from the fact that the stomata are located in the upper epidermis. Here, the impact of rain and contamination is higher than on the lower epidermis. The lotus plant has successfully developed an excellent protection for this delicate epistomatic surface of its leaves.

  20. Reflections Around the Conservation of Sacred Thangkas

    Directory of Open Access Journals (Sweden)

    Sabine Cotte

    2013-10-01

    Full Text Available Tibetan thangkas (Buddhist scroll paintings are created as religious ritual objects. The fact that they are mainly considered as artworks in the Western world impacts on the decisions made for their display and conservation. This article explores the current approach to thangkas in Australian public collections and compares it with the views of contemporary Tibetan Buddhism practitioners. It underlines a few misconceptions at the source of conservation decision-making, and discusses practical outcomes of integrating the sacred dimension into professional practice against the backdrop of conservation’s Codes of Ethics. Conserving living religious heritage requires that professional ethical standards are adaptable to the needs of users. Existing frameworks for the conservation of sacred objects of pre-colonised, indigenous cultures provide useful models for the conservation of thangkas. This article argues that engaging with contemporary cultural groups to conserve religious significance is part of the mission of conservators. This is viewed as an expansion of conservation practice into the social realm, in a search for purposeful conservation that establishes the social relevance of our profession.

  1. Heritage Preservation and Sacred Art After the Second Vatican Council

    Directory of Open Access Journals (Sweden)

    Jitka Jonová

    2017-08-01

    Full Text Available The Constitution Sacrosanctum Concilium specifically contains provisions concerning liturgy but also the preservation of historical monuments and issues of sacred art. There is an urgent question in connection with the development of modern art as to which type of art is suitable for the sacred, liturgical space and which is not. This begs the question as to how to define sacred art. Another problem is raised, however, in connection with the preservation of historic buildings (esp. churches, that being how to reconcile the requirements of church and those of state heritage preservation? Specific questions are presented by disused churches, their sale and subsequent use. There is also the issue of the formation of candidates for the priesthood and priests concerned with preservation of historical monuments and sacred art. The issue will be discussed on the basis of church documents (general and particular and selected relevant examples. The paper focuses on the Sacrosanctum Concilium 44–46, 122–129 in connection not only with preservation of historic church buildings, but with the issue of sacred art (modern art in the Church, sacred furnishings, the commission for sacred art and that of unneeded “redundant” churches: the use of church buildings for other/ secular purposes.

  2. Birds of sacred groves of northern Kerala, India

    Directory of Open Access Journals (Sweden)

    K. M. Jyothi

    2015-12-01

    Full Text Available Sacred groves are patches of vegetation preserved due to  religious or cultural tradition.  They are protected through spiritual beliefs.  Sacred groves provide an excellent abode to the biodiversity of the region where they are located.   Scientific exploration of fauna from sacred groves of India is few and far between.  The present study was conducted to explore the bird diversity and abundance in 15 selected sacred groves of northern Kerala, eight from Kannur District and seven from Kasargod District each.  A total of 111 bird species were observed belonging to 49 families and 16 orders.  The sacred groves of northern Kerala support many of the ‘forest-birds’ such as the Grey Junglefowl Gallus sonneratii, Asian Fairy-bluebird Irena puella, Tickell’s Blue-flycatcher Cyornis tickelliae, Malabar Trogon Harpactes fasciatus, Heart-spotted Woodpecker Hemicircus canente, Malabar Whistling-Thrush Myophonus horsfieldii, Little Spiderhunter Arachnothera longirostra, etc.  The sacred groves of northern Kerala also support two endemic bird species of the Western Ghats, such as the Malabar Grey Hornbill Ocyceros griseus and Rufous Babbler Turdoides subrufa. Five species of raptors and four owl species were reported from the sacred groves of north Kerala during the present study.  The breeding of the White-bellied Sea-Eagle has been reported at Edayilakadu Kavu, a sacred grove in Kasargod District.  The sacred groves of northern Kerala also supported 17 species of long distant migratory birds.  Thazhe Kavu, recorded the Black-headed Ibis Threskiornis melanocephalus, a Near-Threatened bird according to IUCN. 

  3. Background and History of the Lotus japonicus Model Legume System

    DEFF Research Database (Denmark)

    Stougaard, Jens

    2014-01-01

    The combination of favourable biological features, stable transformation procedures, application of genetics and genome-based global approaches has established Lotus japonicus as a model legume and provided a platform for addressing important biological questions often, but not exclusively......, focusing on endosymbiosis. Several important discoveries have been made, and the Lotus community has contributed novel results, promoting our understanding of plant biology as well as our understanding of properties and characteristics typical for plants belonging to the legume family. Progress has been...

  4. Common Sense Approach to the Restoration of Sacred Art

    Directory of Open Access Journals (Sweden)

    Alphonso Lopez Pinto

    2014-12-01

    Full Text Available In this paper, Sacred Art is examined as an imitation of historia. Historia interprets historical human events as empirical, material and real while seeking to understand their moral and spiritual significance. It is from historia that sacred art can be understood, where Christ and the saints are portrayed in the integrity of their human natures united to symbols representing Divinity or grace in order to present a visual/contemplative narrative. Mortimer Adler rightly sees that the vision of the beautiful is inherently contemplative, thus sacred iconography provides a language that can form the common sense of men and women.

  5. Integrated Emergy and Economic Evaluation of Lotus-Root ...

    Science.gov (United States)

    Lotus (Neumbo nucifera, Gaertn) is the most important aquatic vegetable in China, with a cultivation history of over 3000 years. The emergy, energy, material, and money flows of three lotus root cultivation modes in Wanqingsha, Nansha District, Guangzhou, China were examined using Energy Systems Language models and emergy evaluation to better understand their ecological and economic characteristics on multiple spatial and temporal scales. The natural resource foundations, economic characteristics and sustainability of these modes were evaluated and compared. The results showed that although all three modes were highly dependent on purchased emergy inputs, their potential impacts as measured by the local (ELRL) and global (ELRW) environmental loading ratios were less than 1.2 and 0.7, respectively. The lotus-fish mode was the most sustainable with its emergy index of sustainable development (EISD) 2.09 and 2.13 times that of the pure lotus and lotus-shrimp modes, respectively. All three lotus-root production modes had superior economic viability, since their Output/Input ratio ranged from 2.56 to 4.95. The results indicated that agricultural systems may have different environmental impacts and sustainability characteristics at different spatial and temporal scales, and that these impacts and characteristics can be simultaneously explored using integrated emergy and economic evaluations. This study provides some major new insights about agriculture and its potenti

  6. Flavonoid glycosides and alkaloids from the embryos of Nelumbo nucifera seeds and their antioxidant activity.

    Science.gov (United States)

    Jiang, Xi-Lan; Wang, Lun; Wang, En-Jun; Zhang, Guo-Lin; Chen, Bin; Wang, Ming-Kui; Li, Fu

    2018-03-01

    Chemical investigation of the embryos of Nelumbo nucifera afforded four new flavone C-glycosides, named nelumbosides A-D (1-4), together with nine known ones, comprising five flavonoids (5-9) and four alkaloids (10-13). The chemical structures of the new compounds were elucidated by 1D, 2D-NMR and HR-ESI-MS techniques, together with chemical methods. Nelumbosides A-D (1-4) are rarely present in naturally occurring flavone C-glycosides featuring a 4-hydroxystyrene unit connected to the flavonoid skeleton. Compounds 2-13 were evaluated for their antioxidant activity by ABTS and DPPH radical-scavenging assay. Among them, compounds 2, 6, 7 and 11 exhibited strong scavenging activity with SC 50 values ranging from 12.07 to 25.68μM compared with the positive control l-ascorbic acid. Copyright © 2018 Elsevier B.V. All rights reserved.

  7. Stravinsky, Igor: Le Sacre du Printemps / Pierre-E. Barbier

    Index Scriptorium Estoniae

    Barbier, Pierre-E.

    1996-01-01

    Uuest heliplaadist "Stravinsky, Igor: Le Sacre du Printemps. Requiem Canticles. Canticum sacrum. Variations sur un choral de Bach. Orchestre de la Suisse Romande, Neeme Järvi" Chandos CHAN 9 408, distribution Media 7 (SD:160F)

  8. Purification and utilization of garlic processing wastewater in lotus pond wetlands

    OpenAIRE

    Jun Pang; Xue-ling Feng; Xiu-feng Wang

    2014-01-01

    Based on the experiments of utilization of garlic processing wastewater in a lotus pond, this study demonstrates that lotus pond wetlands have a remarkable ability to remove organic pollutants and decrease chemical oxygen demand (CODCr), biochemical oxygen demand (BOD5), and suspended substances (SS) in garlic processing wastewater. Results also show evident effects of lotus roots on absorption of NH3-N. The pH value in a lotus pond with wastewater discharged was relatively stable. The water ...

  9. Four new dammarane saponins from Zizyphus lotus.

    Science.gov (United States)

    Maciuk, Alexandre; Lavaud, Catherine; Thépenier, Philippe; Jacquier, Marie-José; Ghédira, Kamel; Zèches-Hanrot, Monique

    2004-10-01

    Five dammarane-type saponins were isolated by means of centrifugal partition chromatography from the leaves of Zizyphus lotus. Their structures were elucidated using a combination of 1D and 2D 1H and 13C NMR spectra and mass spectroscopy. One of these glycosides is the known jujuboside B (5). Three are new jujubogenin glycosides, identified as 3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranosyljujubogenin-20-O-(2,3,4-O-triacetyl)-alpha-L-rhamnopyranoside (1), 3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranosyljujubogenin-20-O-alpha-L-rhamnopyranoside (2), and 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[(4-sulfo)-beta-D-glucopyranosyl-(1-->3)]-alpha-L-arabinopyranosyljujubogenin (3). The last is a new sulfated derivative of jujubasaponine IV, identified as 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[(4-sulfo)-beta-D-glucopyranosyl-(1-->3)]-beta-D-galactopyranosyl-(20R,22R)-16beta,22:16alpha,30-diepoxydammar-24-ene-3beta,20-diol (4).

  10. The links between protected areas, faiths, and sacred natural sites.

    Science.gov (United States)

    Dudley, Nigel; Higgins-Zogib, Liza; Mansourian, Stephanie

    2009-06-01

    Most people follow and are influenced by some kind of spiritual faith. We examined two ways in which religious faiths can in turn influence biodiversity conservation in protected areas. First, biodiversity conservation is influenced through the direct and often effective protection afforded to wild species in sacred natural sites and in seminatural habitats around religious buildings. Sacred natural sites are almost certainly the world's oldest form of habitat protection. Although some sacred natural sites exist inside official protected areas, many thousands more form a largely unrecognized "shadow" conservation network in many countries throughout the world, which can be more stringently protected than state-run reserves. Second, faiths have a profound impact on attitudes to protection of the natural world through their philosophy, teachings, investment choices, approaches to land they control, and religious-based management systems. We considered the interactions between faiths and protected areas with respect to all 11 mainstream faiths and to a number of local belief systems. The close links between faiths and habitat protection offer major conservation opportunities, but also pose challenges. Bringing a sacred natural site into a national protected-area system can increase protection for the site, but may compromise some of its spiritual values or even its conservation values. Most protected-area managers are not trained to manage natural sites for religious purposes, but many sacred natural sites are under threat from cultural changes and habitat degradation. Decisions about whether or not to make a sacred natural site an "official" protected area therefore need to be made on a case-by-case basis. Such sites can play an important role in conservation inside and outside official protected areas. More information about the conservation value of sacred lands is needed as is more informed experience in integrating these into wider conservation strategies. In

  11. Theoretical Explanation of the Lotus Effect: Superhydrophobic Property Changes by Removal of Nanostructures from the Surface of a Lotus Leaf.

    Science.gov (United States)

    Yamamoto, Minehide; Nishikawa, Naoki; Mayama, Hiroyuki; Nonomura, Yoshimune; Yokojima, Satoshi; Nakamura, Shinichiro; Uchida, Kingo

    2015-07-07

    Theoretical study is presented on the wetting behaviors of water droplets over a lotus leaf. Experimental results are interpreted to clarify the trade-offs among the potential energy change, the local pinning energy, and the adhesion energy. The theoretical parameters, calculated from the experimental results, are used to qualitatively explain the relations among surface fractal dimension, surface morphology, and dynamic wetting behaviors. The surface of a lotus leaf, which shows the superhydrophobic lotus effect, was dipped in ethanol to remove the plant waxes. As a result, the lotus effect is lost. The contact angle of a water drop decreased dramatically from 161° of the original surface to 122°. The water droplet was pinned on the surface. From the fractal analysis, the fractal region of the original surface was divided into two regions: a smaller-sized roughness region of 0.3-1.7 μm with D of 1.48 and a region of 1.7-19 μm with D of 1.36. By dipping the leaf in ethanol, the former fractal region, characterized by wax tubes, was lost, and only the latter large fractal region remained. The lotus effect is attributed to a surface structure that is covered with needle-shaped wax tubes, and the remaining surface allows invasion of the water droplet and enlarges the interaction with water.

  12. The preservation of lotus seeds by irradiation technique

    International Nuclear Information System (INIS)

    Lan Junming; Tang Chuandao

    1990-08-01

    The preservation test results of lotus seeds by gamma-ray irradiation technique are introduced. The results showed that the sitophilus, penicillus and aspergillus oryzae caused the great damage of lotus seeds. It was found that the radiation doses of deactivation for the insects and the mould were 0.8 kGy and 5 kGy respectively. The irradiated seeds packaged in the cloth bags and stored in the storehouse were not damaged by the insects and moulds for three years. The measured resutls also showed that the nutrient compositions were not changed and the pathogenic bacteria were not found. The irradiated lotus seeds are healthy and safe to the people

  13. A superhydrophobic dual-scale engineered lotus leaf

    Science.gov (United States)

    Kim, Donghyun; Kim, Joonwon; Park, Hyun C.; Lee, Kun-H.; Hwang, Woonbong

    2008-01-01

    A surface was created with the same superhydrophobic property as the lotus leaf (lotus effect) by dipping of sandblasted porous alumina into polytetrafluoroethylene (PTFE, Teflon®: DuPont™) solution. The fabricated engineered lotus leaf had PTFE dual-scale structures. This fabrication process has several advantages, including low fabrication cost, simplicity and easy coverage of a large area. The sandblasted porous alumina template was fabricated by sandblasting of an aluminum sheet and anodization in oxalic acid. To obtain PTFE dual-scale structures, PTFE replication based on the dipping method was used, with a 0.3 w% PTFE solution. To remove the aluminum and alumina layers, wet etching by chromic and phosphoric acid mixed solution and liquid HgCl2 solution was used. The fabricated surface has a superhydrophobic property whose apparent contact angle of the PTFE dual-scale structures was approximately 165° and sliding angle is less than 2°.

  14. Kaempferol Isolated from Nelumbo nucifera Inhibits Lipid Accumulation and Increases Fatty Acid Oxidation Signaling in Adipocytes.

    Science.gov (United States)

    Lee, Bonggi; Kwon, Misung; Choi, Jae Sue; Jeong, Hyoung Oh; Chung, Hae Young; Kim, Hyeung-Rak

    2015-12-01

    Stamens of Nelumbo nucifera Gaertn have been used as a Chinese medicine due to its antioxidant, hypoglycemic, and antiatherogenic activity. However, the effects of kaempferol, a main component of N. nucifera, on obesity are not fully understood. We examined the effect of kaempferol on adipogenesis and fatty acid oxidation signaling pathways in 3T3-L1 adipocytes. Kaempferol reduced cytoplasmic triglyceride (TG) accumulation in dose and time-dependent manners during adipocyte differentiation. Accumulation of TG was rapidly reversed by retrieving kaempferol treatment. Kaempferol broadly decreased mRNA or protein levels of adipogenic transcription factors and their target genes related to lipid accumulation. Kaempferol also suppressed glucose uptake and glucose transporter GLUT4 mRNA expression in adipocytes. Furthermore, protein docking simulation suggests that Kaempferol can directly bind to and activate peroxisome proliferator-activated receptor (PPAR)-α by forming hydrophobic interactions with VAL324, THR279, and LEU321 residues of PPARα. The binding affinity was higher than a well-known PPARα agonist fenofibrate. Consistently, mRNA expression levels of PPARα target genes were increased. Our study indicates while kaempferol inhibits lipogenic transcription factors and lipid accumulation, it may bind to PPARα and stimulate fatty acid oxidation signaling in adipocytes.

  15. Relationship between seed moisture content and acquisition of impermeability in Nelumbo nucifera (Nelumbonaceae

    Directory of Open Access Journals (Sweden)

    Ganesh K. Jaganathan

    Full Text Available ABSTRACT Seeds of Nelumbo nucifera do not imbibe water, and thus have physical dormancy (PY. However, a proportion of seeds are permeable to water, and so we hypothesized that variation in moisture content is a reason for the development of both permeable and impermeable seeds. The permeable proportion of seeds present in a lot collected from Suzhou, China, was separated using an imbibition test. The permeable proportion had an average moisture content of 15.6 %, compared with 8.5 % for impermeable seeds. Drying permeable seeds above silica gel to 10 % and 8 % f. wb., resulted in 77 and 100 % impermeable seeds, respectively, compared with no impermeable seeds at 15 % moisture content. Dried to 10 % moisture content, and incubated above water in an airtight container, 46 % of the seeds reverse impermeability. Permeable seeds with 15 % moisture content maintained above LiCl2 (RH=70 % did not develop impermeability after three months of storage. The seeds dried to 6 % moisture content and stored above water in an airtight container showed no increase in moisture. Based on these results, we conclude that there is a strong relationship between moisture content and the onset of impermeability in this species.

  16. Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD

    Directory of Open Access Journals (Sweden)

    Gahee Ryu

    2017-01-01

    Full Text Available Nelumbo nucifera has a variety of biological activities. So it was importantly used as various herbal medicines since traditional times. A simple, fast, and sensitive high-performance liquid chromatographic (HPLC method was developed in this study for efficient quality control of N. nucifera. Four different compounds, including neferine, 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl methyl]-2-methyl-7-isoquinolinol, 1-hydroxy-2-methylpropene, and 3-(prop-1-enylbenzene-1,2,4,5-tetrol, were simultaneously determined. The four compounds were isolated through a Dionex C18 column by gradient elution with 0.1% TFA-water and methanol. The flow rate was 1.0 mL/min, and the wavelength was detected at 205, 254, 280, and 330 nm. The chromatograms were acquired at 205 nm. The four compounds showed good linear relationships (r2>0.96 over five different concentrations, and an average recovery of the method ranged from 96.27% to 108.78%. Through the analysis validation test and application of the method, the optimized conditions verified that it is efficient to isolate the compounds of N. nucifera seed embryos.

  17. The Development of the Virtual Learning Media of the Sacred Object Artwork

    Science.gov (United States)

    Nuanmeesri, Sumitra; Jamornmongkolpilai, Saran

    2018-01-01

    This research aimed to develop the virtual learning media of the sacred object artwork by applying the concept of the virtual technology in order to publicize knowledge on the cultural wisdom of the sacred object artwork. It was done by designing and developing the virtual learning media of the sacred object artwork for the virtual presentation.…

  18. Antiinflammatory and analgesic activities of Zizyphus lotus root barks.

    Science.gov (United States)

    Borgi, W; Ghedira, K; Chouchane, N

    2007-01-01

    The root barks of Zizyphus lotus were extracted with water, chloroform, ethyl acetate and methanol to determine their antiinflammatory and analgesic activities. Aqueous extract (50, 100 and 200 mg/kg) given intraperitoneally (i.p.) showed a significant and dose-dependent antiinflammatory and analgesic activity.

  19. Local evidence for tolerance of birdsfoot trefoil (Lotus corniculatus ...

    African Journals Online (AJOL)

    The pattern of yield response of birdsfoot trefoil (Lotus corniculatus) cv. San Gabriel to soil amendment with lime and superphosphate on a virgin acid soil indicated that this legume is tolerant of local acid soils and moderately tolerant of low levels of extractable phosphorus. Keywords: birdsfoot trefoil; legumes; levels; lime; ...

  20. Bibles and Other Sacred Writings in Special Media. Reference Circular.

    Science.gov (United States)

    Nussbaum, Ruth, Comp.; O'Connor, Catherine, Comp.; Herndon, James, Comp.; Emanuel, Shirley, Comp.

    This reference circular lists Bibles and sacred texts of many world religions, in a variety of languages, translations, and versions, that are available in special media. Commentaries, concordances, liturgies, prayer books, hymnals, and magazines are also listed. Priority was given to citing complete works; portions of works are listed if they are…

  1. Survey Of Indigenous Tree Species In Osun Sacred Grove, Osun ...

    African Journals Online (AJOL)

    A botanical survey of indigenous tree species present in core regions of the Osun sacred grove, Osun state, Nigeria,was conducted. A total of eighty (80) tree species belonging to sixty-six (66) genera and twenty seven (27) families were identified and recorded. The family Fabaceae had the largest number of tree species ...

  2. Stealing the sacred: Why 'global heritage' discourse is perceived as ...

    African Journals Online (AJOL)

    Stealing the sacred: Why 'global heritage' discourse is perceived as a frontal attack on local heritage-making in Madagascar. ... Asked Questions about PDFs. Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download ...

  3. Eboo Patel (2012). Sacred Ground: Pluralism, Prejudice and the ...

    African Journals Online (AJOL)

    more genuine and lasting interfaith cooperation. While Sacred Ground focuses on the. United States, Patel's analysis of interreligious conflict and his prescriptions for promoting interfaith cooperation have broad relevance for international application in today's increasingly globally connected and religiously diverse world.

  4. Biodiversity Conservation and the Sacred Forests of Emohua, Rivers ...

    African Journals Online (AJOL)

    These sacred sites are regarded as the temples of god by the local people due to their beliefs, but technically the forests are centres of biodiversity. In recent times, anthropogenic interference activities expose the forest to threat and challenges which make the development and adoption of conservation strategies inevitable.

  5. Trampling the Sacred: Multicultural Education as Pedagogical Racism

    Science.gov (United States)

    Lovern, Lavonna Lea

    2012-01-01

    The following paper is a critical examination involving the misuse of sacred cultural tools and practices in the name of multicultural education. Native American practices are identified to illustrate how such inappropriate usages promote pedagogical racism. The misuse continues the hegemonic distribution of social capital. Through the…

  6. A Sacred Trust: The Safety of Our Children.

    Science.gov (United States)

    Momentum, 2002

    2002-01-01

    Provides the text of the National Catholic Educational Association Statement on Child Abuse. This document, titled A Sacred Trust: The Safety of Our Children, was generated in response to the growing number of both confirmed and alleged cases of pedophilia and sexual abuse within the Catholic Church. (RC)

  7. Geographical Assessment of Sacred Groves in Bolpur Sub-division ...

    African Journals Online (AJOL)

    Dr Osondu

    without eating. In other groves, dead woods, dried leaves may be picked up but the fresh leaves and its green or brown branches are never cut, for instance,' kankalitala' Sacred ... elephant eye, snake head, natural hole within a tree, naturally emergence of .... Rahati: an ethnobotanical study of the forests preserved on ...

  8. The sacred geography of Kapila: the Kapilasrama of Sidhpur

    Directory of Open Access Journals (Sweden)

    Knut A. Jacobsen

    2003-01-01

    Full Text Available To most scholars of Hinduism, the sage Kapila is a person associated only with ancient India and known mainly as the mythical founder of the Sāmkhya system of religious thought. This is the Kapila whose teaching is known through Yuktidīpikā, the Sāmkhyakārikā by Isvarakrsna and other Sāmkhya texts and the tradition of technical commentaries on them. In India this Kapila belongs to a scholarly tradition preserved mainly by pandits with a knowledge of Sanskrit and, for the last hundred years, also by professors in the Indian university system. In this article, the symbolic significance of one of the most important pilgrimage centres connected with Kapila, Sidhpur in Gujarat, is explored. The close connection between the sacred narratives and the rituals performed at the pilgrimage centre is a significant feature of the sacred places devoted to Kapila. At every place of pilgrimage to Kapila there are narratives about him which account for the sacredness of the place. These narratives belong to the geography of Hindu India as much as to the mythology of the Hindu tradition. The life history of Kapila is engraved in a sacred landscape. The place where Kapila was born, the place where he gave the sacred knowledge of ultimate reality to his mother, the different places where he performed tapas, the place where he killed the sons of King Sagara are all part of India's imagined landscape. The promise of the Kapila pilgrimage sites is that these places have power in themselves to remove moral impurity and grant moksa to the pilgrims. The sacred narratives of Kapila function to make this promise trustworthy.

  9. Rapid validated HPTLC method for estimation of betulinic acid in Nelumbo nucifera (Nymphaeaceae) rhizome extract.

    Science.gov (United States)

    Mukherjee, Debajyoti; Kumar, N Satheesh; Khatua, Taraknath; Mukherjee, Pulok K

    2010-01-01

    Betulinic acid (pentacyclic triterpenoid) is an important marker component present in Nelumbo nucifera Gaertn. rhizome. N. nucifera rhizome has several medicinal uses including hypoglycaemic, antidiarrhoeal, antimicrobial, diuretic, antipyretic, psychopharmacological activities. To establish a simple, sensitive, reliable, rapid and validated high-performance thin-layer chromatography method for estimation of betulinic acid in hydro-alcoholic extract of N. nucifera Gaertn. rhizome. The separation was carried out on a thin-layer chromatography aluminium plate pre-coated with silica gel 60F(254) , eluted with chloroform, methanol and formic acid (49 : 1 : 1 v/v). Post chromatographic derivatisation was done with anisaldehyde-sulphuric acid reagent and densitometric scanning was performed using a Camag TLC scanner III, at 420 nm. The system was found to produce a compact spot for betulinic acid (R(f) = 0.30). A good linear precision relationship between the concentrations (2-10 µg) and peak areas were obtained with the correlation coefficient (r) of 0.99698. The limit of detection and limit of quantification of betulinic acid were detected to be 0.4 and 2.30 µg per spot. The percentage of recovery was found to be 98.36%. The percentage relative standard deviations of intra-day and inter-day precisions were 0.82-0.394 and 0.85-0.341, respectively. This validated HPTLC method provides a new and powerful approach to estimate betulinic acid as phytomarker in the extract. Copyright © 2010 John Wiley & Sons, Ltd.

  10. Decomposition dynamic of two aquatic macrophytes Trapa bispinosa Roxb. and Nelumbo nucifera detritus.

    Science.gov (United States)

    Zhou, Xiaohong; Feng, Deyou; Wen, Chunzi; Liu, Dan

    2018-03-29

    In freshwater ecosystems, aquatic macrophytes play significant roles in nutrient cycling. One problem in this process is nutrient loss in the tissues of untimely harvested plants. In this study, we used two aquatic species, Nelumbo nucifera and Trapa bispinosa Roxb., to investigate the decomposition dynamics and nutrient release from detritus. Litter bags containing 10 g of stems (plus petioles) and leaves for each species detritus were incubated in the pond from November 2016 to May 2017. Nine times litterbags were retrieved on days 6, 14, 25, 45, 65, 90, 125, 145, and 165 after the decomposition experiment for the monitoring of biomass loss and nutrient release. The results suggested that the dry masses of N. nucifera and T. bispinosa decomposed by 49.35-69.40 and 82.65-91.65%, respectively. The order of decomposition rate constants (k) is as follows: leaves of T. bispinosa (0.0122 day -1 ) > stems (plus petioles) of T. bispinosa (0.0090 day -1 ) > leaves of N. nucifera (0.0060 day -1 ) > stems (plus petioles) of N. nucifera (0.0030 day -1 ). Additionally, the orders of time for 50% dry mass decay, time for 95% dry mass decay, and turnover rate are as follows: leaves  0.05). In addition, the decomposition time had also significant effects on the detritus decomposition dynamic and nutrient release. However, the contributors of species and decomposition time on detritus decomposition were significantly different on the basis of their F values of two-way ANOVA results. This study can provide scientific bases for the aquatic plant scientific management in freshwater ecosystems of the East region of China.

  11. Facile fabrication of a lotus-effect composite coating via wrapping silica with polyurethane

    Energy Technology Data Exchange (ETDEWEB)

    Su Changhong, E-mail: suchhnju@yahoo.com.cn [Department of Material Science, School of Material Science and Engineering, Shandong University of Technology, Zhangzou Road 22, Zibo 255049 (China)

    2010-01-15

    A lotus-effect coating was fabricated by wrapping micro-silica and nano-silica with polyurethane (PU) and subsequent spraying. The coating shows the similar self-cleaning property as lotus leaves: the contact angle is as large as 168 deg. and the sliding angle is as low as 0.5 deg. Surface morphology of the coating was studied with scanning electron microscopy and atomic force microscopy. The composite coating shows the similar structure as lotus leaves.

  12. SACRED LANDSCAPES AS REPOSITORIES OF BIODIVERSITY. A CASE STUDY FROM THE HARIYALI DEVI SACRED LANDSCAPE, UTTARAKHAND

    Directory of Open Access Journals (Sweden)

    Yogesh GOKHALE

    2011-03-01

    Full Text Available The present study was carried out in the Hariyali Devi sacred landscape of Garhwal Himalaya in Uttarakhand State of India. The study area falls under the jurisdiction of the Forest Department, having the status of reserve forest. The land scape is dedicated to the deity “Hariyali Devi” and that plays a major role in conserving the biodiversity of this land scape. Taboos, rituals and socio-cultural practices are associated with conservation practices. The study recorded 98 plant species, representing 88 genera and 46 families with different economic values. The dominant family was Rosaceae, which recorded the highest (10 number of species. Out of 98 plant species the dominant life form contribution was of herbs (52, shrubs (26 and tree species (21. Almost 82 plants species in the landscape are of medicinal importance, 15 species are used for timber and construction purposes, 19 species with different edible plant parts, such as fruits, flowers, seeds and rhizomes. The information about the uses/economic value of different plant species was gathered directly by interviewing knowledgeable elderly local villagers (including women.

  13. Superhydrophobic surfaces: From the lotus leaf to the submarine

    Science.gov (United States)

    Samaha, Mohamed A.; Tafreshi, Hooman Vahedi; Gad-el-Hak, Mohamed

    2012-01-01

    In this review we discuss the current state of the art in evaluating the fabrication and performance of biomimetic superhydrophobic materials and their applications in engineering sciences. Superhydrophobicity, often referred to as the lotus effect, could be utilized to design surfaces with minimal skin-friction drag for applications such as self-cleaning and energy conservation. We start by discussing the concept of the lotus effect and continue to present a review of the recent advances in manufacturing superhydrophobic surfaces with ordered and disordered microstructures. We then present a discussion on the resistance of the air-water interface to elevated pressures—the phenomenon that enables a water strider to walk on water. We conclude the article by presenting a brief overview of the latest advancements in studying the longevity of submerged superhydrophobic surfaces for underwater applications.

  14. Microscopic observations of condensation of water on lotus leaves

    Science.gov (United States)

    Cheng, Yang-Tse; Rodak, Daniel E.; Angelopoulos, Anastasios; Gacek, Ted

    2005-11-01

    We report an in situ observation of water condensation and evaporation on lotus leaf surfaces inside an environmental scanning electron microscope. The real-time observation shows, at the micrometer length scale, how water drops grow to large contact angles during water condensation, and decrease in size and contact angle during the evaporation phase of the experiment. To rationalize the observations, we propose a geometric model for liquid drops on rough surfaces when the size of the drop and surface roughness scale are comparable. This model suggests that when drop size and surface roughness are of the same magnitude, such as micrometer size water drops on lotus leaves, well-known equations for wetting on rough surfaces may not be applicable.

  15. Lotus Dust Mitigation Coating and Molecular Adsorber Coating

    Science.gov (United States)

    O'Connor, Kenneth M.; Abraham, Nithin S.

    2015-01-01

    NASA Goddard Space Flight Center has developed two unique coating formulations that will keep surfaces clean and sanitary and contain contaminants.The Lotus Dust Mitigation Coating, modeled after the self-cleaning, water-repellant lotus leaf, disallows buildup of dust, dirt, water, and more on surfaces. This coating, has been successfully tested on painted, aluminum, glass, silica, and some composite surfaces, could aid in keeping medical assets clean.The Molecular Adsorber Coating is a zeolite-based, sprayable molecular adsorber coating, designed to prevent outgassing in materials in vacuums. The coating works well to adsorb volatiles and contaminates in manufacturing and processing, such as in pharmaceutical production. The addition of a biocide would also aid in controlling bacteria levels.

  16. Radiation Resistant Hybrid Lotus Effect Photoelectrocatalytic Self-Cleaning Anti-Contamination Coatings, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — This project will develop radiation resistant hybrid Lotus Effect photoelectrocatalytic self-cleaning anti-contamination coatings for application to Lunar...

  17. Lotus leaf alleviates hyperglycemia and dyslipidemia in animal model of diabetes mellitus

    OpenAIRE

    Kim, Ah-Rong; Jeong, Soo-Mi; Kang, Min-Jung; Jang, Yang-Hee; Choi, Ha-Neul; Kim, Jung-In

    2013-01-01

    The purpose of this study was to investigate the effects of lotus leaf on hyperglycemia and dyslipidemia in animal model of diabetes. Inhibitory activity of ethanol extract of lotus leaf against yeast ?-glucosidase was measured in vitro. The effect of lotus leaf on the postprandial increase in blood glucose levels was assessed in streptozotocin-induced diabetic rats. A starch solution (1 g/kg) with and without lotus leaf extract (500 mg/kg) was administered to the rats after an overnight fast...

  18. Fractal Surfaces of Molecular Crystals Mimicking Lotus Leaf with Phototunable Double Roughness Structures.

    Science.gov (United States)

    Nishimura, Ryo; Hyodo, Kengo; Sawaguchi, Haruna; Yamamoto, Yoshiaki; Nonomura, Yoshimune; Mayama, Hiroyuki; Yokojima, Satoshi; Nakamura, Shinichiro; Uchida, Kingo

    2016-08-17

    Double roughness structure, the origin of the lotus effect of natural lotus leaf, was successfully reproduced on a diarylethene microcrystalline surface. Static superwater-repellency and dynamic water-drop-bouncing were observed on the surface, in the manner of natural lotus leaves. Double roughness structure was essential for water-drop-bouncing. This ability was not observed on a single roughness microcrystalline surface showing the lotus effect of the same diarylethene derivative. The double roughness structure was reversibly controlled by alternating irradiation with UV and visible light.

  19. Radiation Resistant Hybrid Lotus Effect Photoelectrocatalytic Self-Cleaning Anti-Contamination Coatings Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This project will develop radiation resistant hybrid Lotus Effect photoelectrocatalytic self-cleaning anti-contamination coatings for application to Lunar...

  20. Purification and utilization of garlic processing wastewater in lotus pond wetlands

    Directory of Open Access Journals (Sweden)

    Jun Pang

    2014-10-01

    Full Text Available Based on the experiments of utilization of garlic processing wastewater in a lotus pond, this study demonstrates that lotus pond wetlands have a remarkable ability to remove organic pollutants and decrease chemical oxygen demand (CODCr, biochemical oxygen demand (BOD5, and suspended substances (SS in garlic processing wastewater. Results also show evident effects of lotus roots on absorption of NH3-N. The pH value in a lotus pond with wastewater discharged was relatively stable. The water quality in the lotus pond reached the class II emission standard, according to the Integrated Wastewater Discharge Standard (GB8978-1996, seven days after pretreated garlic processing wastewater had been discharged into the lotus pond. Garlic processing wastewater irrigation does not produce pollution in the pond sediment and has no negative effect on the growth of lotus roots. Due to utilization of garlic processing wastewater, the output of lotus roots increased by 3.0% to 8.3%, and the quality of lotus roots was improved. Therefore, better purification and utilization results can be achieved.

  1. A rare cause of gastrointestinal phytobezoars: diospyros lotus

    Directory of Open Access Journals (Sweden)

    Ertuğrul Gökhan

    2012-06-01

    Full Text Available Abstract Aim Diospyros Lotus (“Wild Date Palm of Trabzon or Persimmon”, which has been proven to cause phytobezoars, is a widely consumed fruit in the Black Sea and Northeast Anatolia regions of Turkey. The aim of the present study was to investigate the effects of Diospyros Lotus together with other predisposing factors, on the development of gastrointestinal phytobezoars and to discuss the treatment results in comparison to the literature. Material and method The records of 13 patients, who had been admitted to the General Surgery Clinic of Düzce Atatürk State Hospital between August 2008 and August 2011, were retrospectively reviewed. Demographic characteristics, predisposing factors, clinical and radiological findings, diagnostic and therapeutic methods, and the outcomes of the patients were recorded from the patient files. Written informed consent was obtained from each patient for publication of this research article and accompanying images. Results All the patients had a history of consuming Diospyros Lotus. Of the patients, 30,7% had a history of previous gastric surgery, 30,7% had diabetes mellitus and 23% had dental implants. None of the patients had hypothyroidism, which is another predisposing factor for phytobezoars. The phytobezoars were located in the stomach alone in 23% of the patients, whereas 15,3% was detected in the jejunum and stomach, 15,3% was detected in the jejunum alone, and 46,1% was detected in the ileum alone. All patients were treated with surgery, and there were no deaths. Conclusion Gastric phytobezoars are rare. Preventive measures have particular importance in the management of this condition, which is difficult to treat. For this purpose, excessive consumption of herbal nutrients containing a high amount of indigestible fibers such as Diospyros Lotus should be avoided in patients with a history of gastrointestinal surgery or poor oral and dental health.

  2. Induced Mutation in Yellow Lotus by Gamma Irradiation

    International Nuclear Information System (INIS)

    Puripunyavanich, Vichai; Boonsirichai, Kanokporn

    2006-01-01

    Rhizomes of American yellow lotus were irradiated at eht dosed of 0, 10, 20 and 30 Gy, 10 rhizomes per treatment. They were planted in nursery pots. Unirradiated rhizomes gave out new shoots within 3-4 days. The survival rates of the 10, 20 and 30 Gy irradiated rhizomes at one month after transplanted were 80%, 30% and 10%, respectively. The radiation dose that resulted in a 50% death rate (LD 5 0) was approximately 17 Gy. The surviving plant lets were transplanted and grown in pots as the Agricultural Occupation Promotion and Development Center in Chiangmai and Chiangrai for three years. Normally, American yellow lotus does not flower in Thailand. However, a mutant was found to bear flower in Thailand. The mutant flower appeared a little different from the wild-type flower. The tip of its petals was more rounded than the pointy wild-type tip. The mutant will be propagated for clonal production or for use as a par net in breeding crosses with Thai white and pink lotuses

  3. Sacred Values, Strategic Communication, and Military Operations in Afghanistan

    Science.gov (United States)

    2010-02-17

    Axelrod, "Reframing Sacred Values." Negotiation Journal, July 2008, 221. 5 Martha Nussbaum , "In Defense of Universal Values." Lecture. Women and Human...the burka voluntarily for religious or cultural reasons. Nussbaum maintains that people are innately drawn toward obtaining capabilities and liberties...Garrett, Martha Nussbaum on Capabilities and Human Rights. 28 April 2008. http://www.wku.edu/~jan.garrett/ethics/naussbaum.htm (accessed 23 September

  4. Monasteries and tourism: interpreting sacred landscape through gastronomy

    Directory of Open Access Journals (Sweden)

    Silvia Aulet

    2017-01-01

    Full Text Available This article analyses the role of monasteries as a sacred space and how their relationship with tourism depicts a landscape of 'good taste'. Monasteries are examples of both tangible and intangible heritage, and are highly symbolic built spaces that have often become the guardians of tradition. They are strongly embedded within a local cultural landscape, which has determined their historical evolution. Monasteries used to be self-sufficient communities that relied on the resources available in their local environment, e.g. they produced their own wine, which was essential for the celebration of the Eucharist; or they preserved food from their own produce. Gastronomy in monasteries can be a tool to improve tourists' visitor experience, in so far as it respects the values that these sacred spaces represent. This article explores the literature on monasteries as sacred spaces; the relationship between their tangible and intangible heritage attributes; and how monasteries and their heritage are linked to tourism. This is illustrated through examples from Spain.

  5. Sacred values and conflict over Iran's nuclear program

    Directory of Open Access Journals (Sweden)

    Morteza Dehghani

    2010-12-01

    Full Text Available Conflict over Iran's nuclear program, which involves a US-led policy to impose sanctions on Iran, is perceived by each side as a preeminent challenge to its own national security and global peace. Yet, there is little scientific study or understanding of how material incentives and disincentives, such as economic sanctions, psychologically affect the targeted population and potentially influence behaviour. Here we explore the Iranian nuclear program within a paradigm concerned with sacred values. We integrate experiments within a survey of 1997 Iranians. We find that a relatively small but politically significant portion of the Iranian population believes that acquiring nuclear energy has become a sacred value, in the sense that proposed economic incentives and disincentives result in a ``backfire effect'' in which offers of material rewards or punishment lead to increased anger and greater disapproval. This pattern was specific to nuclear energy and did not hold for acquiring nuclear weapons. The present study is the first demonstration of the backfire effect for material disincentives as well as incentives, and on an issue whose apparent sacred nature is recent rather than longstanding.

  6. Why Sacred Lands Are Not Indivisible: The Cognitive Foundations of Sacralising Land

    Directory of Open Access Journals (Sweden)

    Richard Sosis

    2011-02-01

    Full Text Available Numerous political analysts have argued that conflicts over sacred land are intractable. These scholars maintain that sacred lands are psychologically perceived as indivisible, or alternatively, in the sociological tradition, their indivisibility is a social fact. Moreover, religious beliefs are viewed as stagnant and resistant to change. Consequently, resolving such conflicts is fraught with difficulty, and even if a truce could be imposed, it would be unstable and violence would eventually erupt. A cognitive and evolutionary account offers a less pessimistic view. Individuals do not conceive of sacred lands in the same way that they conceive of sacred space, such as cemeteries or houses of worship, or sacred objects, such as holy water or prayer beads. Unlike sacred space and objects, whose boundaries are clearly defined, conceptions of sacred land are typically abstract and may bear little resemblance to the contested physical land. While abstract notions of sacred land are indivisible and must remain intact, the physical land is not indivisible, and therefore there is often greater room for negotiation of sacred lands than is generally appreciated.

  7. Activation of an endogenous retrotransposon associated with epigenetic changes in Lotus japonicus

    DEFF Research Database (Denmark)

    Fukai, Eigo; Stougaard, Jens; Hayashi, Makoto

    2013-01-01

    Long terminal repeat retrotransposons occupy a large portion of genomes in flowering plants. In spite of their abundance, the majority are silenced and rarely transpose. One of the examples of a highly active retrotransposon is Lotus Retrotransposon 1(LORE1), of the model legume Lotus japonicus (...

  8. Disgust and the sacred: Do people react to violations of the sacred with the same emotion they react to something putrid?

    Science.gov (United States)

    Kollareth, Dolichan; Russell, James A

    2018-03-01

    Disgust has been hypothesized to be uniquely linked to violations of a distinct moral domain (called divinity, purity, or sacred) aimed at preserving one's body from contamination with pathogens and preserving one's soul from violations of what is sacred. Here we examined whether the same emotion-core disgust-occurs when witnessing both types of violation, and we proposed a specific method for doing so. In two studies (N = 160; 240), American and Indian participants indicated their emotional reaction to (stories depicting) sacred or nonsacred violations, each either with or without pathogens. Both Americans and Indians felt "grossed out" (a term for core disgust) by events with pathogens (whether violations of the sacred or not). They felt disgusted and angered, but not grossed out, by violations of the sacred. For both Americans and Indians, grossed out was never the modal emotion when a sacred violation did not involve pathogens. Results were inconsistent with a focus on any single emotion: sacred violations were associated with several different negative emotions. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

  9. The ecological implications of ancestral religion and reciprocal exchange in a sacred forest in Karendi

    Science.gov (United States)

    Cynthia T. Fowler

    2003-01-01

    This article tells the story of the sacred place named Mata Loko ("River's Source") in Karendi on the western end of the islands of sumba. This ethnographic case of an eastern Indonesian society where the traditional religion of Marapu persists sheds light on questions of how local belief systems are part of environmental adaptions. the use of sacred...

  10. The man, the machine and the sacred: when the virtual reality reenchants the world

    Directory of Open Access Journals (Sweden)

    Olivier NANNIPIERI

    2011-01-01

    Full Text Available The rationality associated with the technical progress were able to let believe that the world became disillusioned. Now, far from disillusioning the world, certain technical devices reveal the sacred dimension inherent to any human activity. Indeed, paradoxically, we shall show that the human-machine interaction producing virtual environments is an experience of the sacred.

  11. The Tunebook That Roars: The Sound and Style of Sacred Harp Singing.

    Science.gov (United States)

    Scholten, James

    1980-01-01

    The Chattahoochee, Georgia, Sacred Harp Musical Convention has been held annually since 1852. Its tunebook uses a shape-note solmization system introduced to America in the seventeenth century. This article describes the history of shape-note tunebooks; some Sacred Harp songs, modern singing events, and teaching techniques. Resources are listed.…

  12. Bird Species Diversity and abundance in the Abiriw Sacred Grove in ...

    African Journals Online (AJOL)

    The sacred grove concept is one of the strategies developed by many human societies to conserve biological resources using a traditional approach. Sacred groves are currently under threat from anthropogenic activities due to lack of enforcement of traditional edicts to check encroachment. The birds inhabiting the Abiriw ...

  13. An encroachment of ecological sacred sites and its threat to the ...

    African Journals Online (AJOL)

    An encroachment of ecological sacred sites and its threat to the interconnectedness of sacred rituals: A case study of the Tonga people in the Gwembe valley. ... of crop production and rain patterns. This study has found out that indigenous peoples' religion is embedded in their understating of ecological sites and rituals.

  14. The influence of sacred beliefs in environmental risk perception and attitudes

    Science.gov (United States)

    Sonya Sachdeva

    2016-01-01

    Elements of the natural world, such as mountains, rivers, and forests, are often seen as sacred in many cultural traditions. Recent conservation movements have even begun to draw on spiritual and religious beliefs to promote issues of environmental sustainability. The straightforward assumption in these cases is that sacred beliefs (compared with secular ones) will...

  15. 77 FR 55860 - Tribal Listening Sessions on Sacred Sites on Federal Lands

    Science.gov (United States)

    2012-09-11

    ... DEPARTMENT OF THE INTERIOR Bureau of Indian Affairs Tribal Listening Sessions on Sacred Sites on... conduct a listening session with Indian tribes to obtain oral and written comments concerning sacred sites located on Federal lands. This session in Tulsa, Oklahoma, is the sixth in a series of listening sessions...

  16. 77 FR 46106 - Tribal Listening Sessions on Sacred Sites on Federal Lands

    Science.gov (United States)

    2012-08-02

    ... DEPARTMENT OF THE INTERIOR Bureau of Indian Affairs Tribal Listening Sessions on Sacred Sites on... conduct listening sessions with Indian tribes to obtain oral and written comments concerning sacred sites... the SUPPLEMENTARY INFORMATION section of this notice for the dates of the tribal listening sessions...

  17. Sacred Space in Community settlement of Kudus Kulon, Central Java, Indonesia

    Science.gov (United States)

    Budi Sardjono, Agung; Rochma Harani, Arnis

    2017-12-01

    The sacred space becomes an important part of the spatial layout of Javanese society, as well as in most houses of the archipelago. This space is related to religious activities, highly respected and usually located in the main place. Kudus Kulon community is a part of Javanese culture in the northern coastal area of Java. Known as a devout Muslim society, sacred space in Kudus Kulon community house becomes an important thing to understand the culture of living in society. The research was conducted by looking at the religious activities of the community and how the space of activity is realized. The research was conducted by qualitative research method. In the Kudus Kulon community, sacred spaces are available in the house itself, in the community grup of houses, and around Kudus Kulon area. In every house, the sacred space can be found in Gedongan and extends to Dalem. In the community group of houses, the sacred space can be found in the neighbourhood praying area, while In Kudus Kulon area, it can be found in Mosque complex and Tower tomb. Physically sacred space is realized by raising the floor height of the space around it. In architectural view, the sacred space is seen on the elevation of the roof or roof angle. Spatially the sacred space occupies a central position and visually represented by a vertical orientation.

  18. Ficus religiosa L. (English: Peepal tree or sacred fig; Hindi: Pippal ...

    Indian Academy of Sciences (India)

    Ficus religiosa L. (English: Peepal tree or sacred fig; Hindi: Pippal) of Moraceae is a large deciduous tree that grows wild as well as cultivated. The picture shows the tree with fresh flush of leaves. The tree is planted chiefly near the temples by Hindus and Buddhists who regard it as sacred. The characteristic heart-shaped ...

  19. Responsibility, God and society: The cry of the other in the sacred ...

    African Journals Online (AJOL)

    The article seeks to respond to the question: What role can the sacred texts play in the construction of a Christian identity that is responsible to the Other in a pluralistic global world? The sacred texts of the Judaic-Christian tradition offer not only an understanding of the wholly otherness of God, but also form the basis of our ...

  20. Marriage in the theology of Martin Luther – worldly yet sacred: An ...

    African Journals Online (AJOL)

    Marriage, according to Martin Luther, is an institution both secular and sacred. It is secular because it is an order of this earthly life. But its institution goes back to the beginning of the human race and that makes marriage sacred, a divine and holy order. It does not – like the sacraments – nourish and strengthen faith or ...

  1. Dyggve's 'Adrio-Byzantine' capitals and the sacred space

    DEFF Research Database (Denmark)

    Fleischer, Jens

    2014-01-01

    Ejnar Dyggve in his 1933 Eigentümlichkeiten und Ursprung der frühmittelaterlichen Architektur in Dalmatien gives a sketchy, yet comprehensive picture of his archaeological research in Salona. According to Dyggve, early medieval architecture in Dalmatia was characterized by oddities (Eigentümlichk......-zone protome capitals in a broader perspective. It is the purpose of this paper to discuss the figured motifs and foliage in so far it is possible on the basis of other forms of architectural sculpture and the sacred space as a vision of Paradise....

  2. Sacred rivers: their spiritual significance in Hindu religion.

    Science.gov (United States)

    Agoramoorthy, Govindasamy

    2015-06-01

    The ancient civilizations in India, China, Egypt and Mesopotamia have flourished due to large rivers that provided water for agriculture over millennia. Egypt was able to grow well because of the Nile. Similarly, Mesopotamia had two rivers namely the Tigris and the Euphrates. Likewise, India and China have several great rivers that continue to support the agrarian culture. This article discusses the sacred significance of rivers in the ancient and contemporary Indian culture with examples from popular Hindu scriptures. It also presents the ancient model of an eco-friendly check dam and its modern application with potential to mitigate future water-related problems across the drylands of India and elsewhere.

  3. Metal matrix composites for sustainable lotus-effect surfaces.

    Science.gov (United States)

    Nosonovsky, Michael; Hejazi, Vahid; Nyong, Aniedi E; Rohatgi, Pradeep K

    2011-12-06

    The lotus effect involving roughness-induced superhydrophobicity is a way to design nonwetting, self-cleaning, omniphobic, icephobic, and antifouling surfaces. However, such surfaces require micropatterning, which is extremely vulnerable to even small wear rates. This limits the applicability of the lotus effects to situations when wear is practically absent. To design sustainable superhydrophobic surfaces, we suggest using metal matrix composites (MMCs) with hydrophobic reinforcement in the bulk of the material, rather than only at its surface. Such surfaces, if properly designed, provide roughness and heterogeneity needed for superhydrophobicity. In addition, they are sustainable, since when the surface layer is deteriorated and removed due to wear, hydrophobic reinforcement and roughness remains. We present a model and experimental data on wetting of MMCs. We also conducted selected experiments with graphite-reinforced MMCs and showed that the contact angle can be determined from the model. In order to decouple the effects of reinforcement and roughness, the experiments were conducted for initially smooth and etched matrix and composite materials. © 2011 American Chemical Society

  4. Experiencing and Teaching Pilgrimage in a Sacred Spaces Course

    Directory of Open Access Journals (Sweden)

    Thomas J. Sienkewicz

    2018-03-01

    Full Text Available As part of an integrated studies curriculum at the author’s college, all juniors must take a Reflections course in which students consider personal values and analyze familiar and unfamiliar systems of thought and belief, in order to explore their own and others’ ideas about the ultimate meaning and purposes of life. “Sacred Places Past and Present”, is a course designed to fulfil this requirement. This course focuses on a number of important religious sites in the ancient Mediterranean and in the modern world, including the Parthenon, Olympia, Delphi, Stonehenge, and Muir Woods. These places are compared and contrasted in terms of what makes them sacred. Two pilgrimage experiences are part of this course: the hajj to Mecca and the Camino de Santiago de Compostela. In the past, the unit on the Camino focused on Emilio Estevez’s 2010 film “The Way”; however, during the summer of 2016, the author walked the Camino de Santiago. As a result, the course was substantially revised to reflect the author’s own personal experiences as a pilgrim. In particular, Paulo Coelho’s The Pilgrimage was incorporated into the course and students were given an opportunity to participate in a one-day pilgrimage walk in western Illinois.

  5. Violence and the Sacred in Georges Bataille's Philosophy

    Directory of Open Access Journals (Sweden)

    A. ZYGMONT

    2015-06-01

    Full Text Available The article considers the concepts of violence and the sacred in the work of the French philosopher Georges Bataille. The author traces back the transformations of the concept of violence throughout the thinker’s carrier, namely in his early literary and philosophical experiences of the late 20s — early 30s, his reports for the College of Sociology (1937–1939 and his «systematic» postwar writings (1945–1962. The author also puts forward the hypothesis of the connection between the concept violence and the concept the sacred and demonstrates that it is most thoroughly represented in the last period of his work, although it may have been noted before. Diachronic consideration of the formation of these concepts allows the author to trace their correlation from the more or less random links mediated by artistic images and the other notions (sacrifi ce, for example to their explicit identification with each other and the systematic development in relation to such concepts as nature, life, being, intimacy, the animal state, expenditure, sacrifice, war and eroticism

  6. Potential Benefits of Jujube (Zizyphus Lotus L.) Bioactive Compounds for Nutrition and Health.

    Science.gov (United States)

    Abdoul-Azize, Souleymane

    2016-01-01

    Zizyphus lotus , belonging to the Rhamnaceae family, is a deciduous shrub which generally grows in arid and semiarid regions of the globe. In traditional medicine, Z. lotus is used as antidiabetes, sedative, bronchitis, and antidiarrhea by local populations. Recently, several scientific reports for health benefit and nutritional potential of bioactive compounds from this jujube have been reported. This plant is rich in polyphenols, cyclopeptide alkaloids, dammarane saponins, vitamins, minerals, amino acids, and polyunsaturated fatty acids. These identified compounds were supposed to be responsible for most of Z. lotus biologically relevant activities including antimicrobial, anti-inflammatory, hypoglycemic, antioxidant, and immunomodulatory effects. The aim of the present review was to give particular emphasis on the most recent findings on biological effects of the major groups of Zizyphus lotus components and their medical interest, notably for human nutrition, health benefit, and therapeutic impacts.

  7. Potential Benefits of Jujube (Zizyphus Lotus L. Bioactive Compounds for Nutrition and Health

    Directory of Open Access Journals (Sweden)

    Souleymane Abdoul-Azize

    2016-01-01

    Full Text Available Zizyphus lotus, belonging to the Rhamnaceae family, is a deciduous shrub which generally grows in arid and semiarid regions of the globe. In traditional medicine, Z. lotus is used as antidiabetes, sedative, bronchitis, and antidiarrhea by local populations. Recently, several scientific reports for health benefit and nutritional potential of bioactive compounds from this jujube have been reported. This plant is rich in polyphenols, cyclopeptide alkaloids, dammarane saponins, vitamins, minerals, amino acids, and polyunsaturated fatty acids. These identified compounds were supposed to be responsible for most of Z. lotus biologically relevant activities including antimicrobial, anti-inflammatory, hypoglycemic, antioxidant, and immunomodulatory effects. The aim of the present review was to give particular emphasis on the most recent findings on biological effects of the major groups of Zizyphus lotus components and their medical interest, notably for human nutrition, health benefit, and therapeutic impacts.

  8. Effects of micro- and nano-structures on the self-cleaning behaviour of lotus leaves

    Science.gov (United States)

    Cheng, Y. T.; Rodak, D. E.; Wong, C. A.; Hayden, C. A.

    2006-03-01

    When rain falls on lotus leaves water beads up with a high contact angle. The water drops promptly roll off the leaves, collecting dirt along the way. This self-cleaning ability or lotus effect has, in recent years, stimulated much research effort worldwide for a variety of applications ranging from self-cleaning window glasses, paints, and fabrics to low friction surfaces. What are the mechanisms giving rise to the lotus effect? Although chemical composition and surface structure are believed important, a systematic experimental investigation of their effects is still lacking. By altering the surface structure of the leaves while keeping their chemical composition approximately the same, we report in this study the influence of micro- and nano-scale structures on the wetting behaviour of lotus leaves. The findings of this work may help design self-cleaning surfaces and improve our understanding of wetting mechanisms.

  9. Identification of genetic components involved in Lotus-endophyte interactions

    DEFF Research Database (Denmark)

    Zgadzaj, Rafal Lukasz

    colonisation of below-ground plant organs. It focused on bacterial endophyte, Rhizobium KAW12, colonisation of spontaneously formed nodules in snf1 mutants and symbiotic signalling mutants in a snf1 background. Additionally, participation of genes required for rhizobial accomodation during endophytic invasion...... was tested by coinoculation experiments with Rhizobium KAW12 and nodule inducing strains or their symbiotically deficient mutants. Such approaches allowed to identify genes possibly involved in host-endophyte recognition. Additionally, bacterial mutants used in these screenings pointed towards...... testing single host-single microsymbiont interactions, an effort was made to study relationships in between plants and the soil microbiome. Comparison of results for the nfr5 mutant of Lotus with results previously obtained for Arabidopsis suggested that plants were able to build specific bacterial...

  10. Image of Iraqis in The Cinema of Sacred Defense

    Directory of Open Access Journals (Sweden)

    Abdollah Givian

    2012-03-01

    Full Text Available Regarding representational function of media, the current dissertation intends to examine the changes in the representation of the image of the Iraqis in the Sacred Defense Cinema. Iraqies have been pictured as the "Others" during last three decades. Due to the importance and role of sacred defense and its cinematic representations of "Self" and "Other" and thereby its contribution in the process of reconstruction of Iranian national identity this research is aimed at studying represented images of Iraqies and its changes during last three decades. To reach this objective I first examined the concept and theories of representation as stated by Richard Dyer and Stuart Hall. Then to complement Hall’s theories I referred to other thinkers in the field of identity theory, ‘self’ and ‘the other’ as stated. In the chapter on methodology I’ve benefited from a combination of the patterns presented by Kate Selbi, Ran Kallory and Rolan Bart to analyze the texts.The findings of this thesis demonstrates that in order to depict the Iraqis in the sixties verbal codes were primarily used whereas in the seventies and the eighties social and technical codes were most used respectively.In the first decade Iraqis were portrayed as those people who are imagined to be cruel, inhuman, in which the emphasis was laid on the impact of the Iraqi’s invasion. In the second decade we see Iraqi’s depicted as dumb and fat people having thick moustaches and rough voices and in the third we see this portrayal to be transformed where Iraqi’s are shown to be our Muslim brothers who have been forced to fight in the war. Having observed, examined and analyzed the films, we found out that when facing ‘the other’, i.e. the Iraqis, the metaphor of the boundary or border was used. This border is seen between ‘self’ and ‘the other’, i.e. the Iranians and the Iraqis in all the chosen movies on the Sacred Defense. In the first decade there was an objective

  11. Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation

    OpenAIRE

    Benammar, Chahid; Hichami, Aziz; Yessoufou, Akadiri; Simonin, Anne-Marie; Belarbi, Meriem; Allali, Hocine; Khan, Naim A

    2010-01-01

    Abstract Background Zizyphus lotus L. (Desf.) also known as Jujube, is a deciduous shrub which belongs to Rhamnaceae family. This plant is used in Algerian traditional medicine for its anti-diabetic, sedative, analgesic, anti-inflammatory and hypoglycaemic activities. In the present study, we determined the concentrations of different vitamins (vitamin A, C and E) and fatty acids in root, stem, leaves, fruit pulp and seed of Zizyphus lotus L. (Desf.) and assessed the effects of their aqueous ...

  12. Potential Benefits of Jujube (Zizyphus Lotus L.) Bioactive Compounds for Nutrition and Health

    OpenAIRE

    Abdoul-Azize, Souleymane

    2016-01-01

    Zizyphus lotus, belonging to the Rhamnaceae family, is a deciduous shrub which generally grows in arid and semiarid regions of the globe. In traditional medicine, Z. lotus is used as antidiabetes, sedative, bronchitis, and antidiarrhea by local populations. Recently, several scientific reports for health benefit and nutritional potential of bioactive compounds from this jujube have been reported. This plant is rich in polyphenols, cyclopeptide alkaloids, dammarane saponins, vitamins, minerals...

  13. Classical and molecular genetics of the model legume Lotus japonicus.

    Science.gov (United States)

    Jiang, Q; Gresshoff, P M

    1997-01-01

    The model legume Lotus japonicus was demonstrated to be amenable to classical and molecular genetic analysis, providing the basis for the genetic dissection of the plant processes underlying nodulation and nitrogen fixation. We have developed an efficient method for the sexual hybridization of L. japonicus and obtained F1 progeny derived from a cross of L. japonicus B-129-S9 Gifu x B-581 Funakura. Over half of the cross-pollinations resulted in fertile hybrid seed, which were confirmed morphologically and by single arbitrary primer DNA amplification polymorphisms using the DAF technique. Molecular and morphological markers segregated in true Mendelian fashion in a F2 population of 100 plants. Several DAF loci were linked using the MAPMAKER software to create the first molecular linkage groups of this model legume. The mapping population was advanced to generate a set of immortal recombinant inbred lines (F6; RILs), useful for sharing plant material fixed genetically at most genomic regions. Morphological loci for waved stem shape (Ssh), dark leaf color (Lco), and short flowering period (Fpe) were inherited as single dominant Mendelian loci. DAF markers were dominant and were detected between Gifu and Funakura at about one per primer, suggesting that the parents are closely related. One polymorphism (270G generated by single octomer primer 8.6m) was linked to a morphological locus controlling leaf coloration. The results demonstrate that (i) Lotus japonicus is amenable to diploid genetic analysis, (ii) morphological and molecular markers segregate in true diploid fashion, (iii) molecular polymorphisms can be obtained at a reasonable frequency between the related Gifu and Funakura lines, and iv) the possibility exists for map-based cloning, marker assisted selection and mapping of symbiotic mutations through a genetic and molecular map.

  14. Superhydrophobic Surfaces Developed by Mimicking Hierarchical Surface Morphology of Lotus Leaf

    Directory of Open Access Journals (Sweden)

    Sanjay S. Latthe

    2014-04-01

    Full Text Available The lotus plant is recognized as a ‘King plant’ among all the natural water repellent plants due to its excellent non-wettability. The superhydrophobic surfaces exhibiting the famous ‘Lotus Effect’, along with extremely high water contact angle (>150° and low sliding angle (<10°, have been broadly investigated and extensively applied on variety of substrates for potential self-cleaning and anti-corrosive applications. Since 1997, especially after the exploration of the surface micro/nanostructure and chemical composition of the lotus leaves by the two German botanists Barthlott and Neinhuis, many kinds of superhydrophobic surfaces mimicking the lotus leaf-like structure have been widely reported in the literature. This review article briefly describes the different wetting properties of the natural superhydrophobic lotus leaves and also provides a comprehensive state-of-the-art discussion on the extensive research carried out in the field of artificial superhydrophobic surfaces which are developed by mimicking the lotus leaf-like dual scale micro/nanostructure. This review article could be beneficial for both novice researchers in this area as well as the scientists who are currently working on non-wettable, superhydrophobic surfaces.

  15. 78 FR 39062 - Group Lotus plc; Modification of a Temporary Exemption From an Advanced Air Bag Requirement of...

    Science.gov (United States)

    2013-06-28

    ...This notice modifies the temporary exemption granted to Group Lotus plc (Lotus) on March 8, 2013. The agency granted Lotus an exemption from the higher maximum speed (56 km/h (35 mph)) belted test requirement using 5th percentile adult female dummies for the front passenger position of its Evora model for the period from March 8, 2013 to March 8, 2014. The agency is modifying the dates of the exemption to account for vehicles Lotus manufactured before the exemption went into effect.

  16. Douglas E. Cowan: Sacred Terror. Religion and Horror on the Silver Screen

    Directory of Open Access Journals (Sweden)

    Sybil A. Thornton

    2016-10-01

    Full Text Available This contribution offers a review of: Douglas E. Cowan: Sacred Terror. Religion and Horror on the Silver Screen. Waco, Texas: Baylor University Press, 2016. 325 pages, $29,95, ISBN (paperback: 978-1-4813-0490-0.

  17. Detecting Adulteration in Lotus Honey Using a Machine Olfactory System

    Directory of Open Access Journals (Sweden)

    M Hajinezhad

    2017-10-01

    Full Text Available Introduction Honey is a supersaturated sugar and viscose solution taken from the nectar of flowers, collected and modified by honeybees. Many producers of honey add some variety of sugars in honey that make difficulties with detection of adulterated and pure honey. Flavor is one of the most important parameters in the classification of honey samples and the smell emitted by the honey depending on the different flowers and constituents that could be different. This causes using an electronic nose system to detect honey adulteration. Materials and Methods Honey samples used in this study were lotus honey that was supplied from a market in Karaj city, Alborz province, Iran. Adulterated honey, along with percentages of fraud (by weight of zero, 20, 35 and 50 percent, was prepared by mixing sugar syrup. Each group of samples, nine times were tested by the electronic nose system. The proposed system, consists of six metal oxide semiconductor sensors, sensor chamber, sample chamber, data acquisition systems, power supply, electric valves, and pumps. Electronic nose is planned for three-phase system baseline correction, the smell of sample injection and cleaning of the sensor and sample chambers with clean air (Oxygen. Responses of the sensors were collected and stored in 420 seconds by a data acquisition system and LabView ver 2012 software. We used fractional method in this study, in order to improve the quality of the information available and to optimize the array output before passing it on to the pattern recognition system. Linear Discriminant Analysis (LDA, Principal Component Analysis (PCA and Artificial neural network (ANN were the methods used for analyzing and recognizing pattern of electronic nose signals. Data processing was carried out using Microsoft Excel, neuralsolution 5 and Unscrambler X 10.3 (CAMO AS, Norway. Results and Discussion PCA Results PCA reduces the complexity of the data-set and is performed with no information on the

  18. Technical Limitations in Merging Secular and Sacred Functions in Monumental Churches

    Science.gov (United States)

    Piatkowska, Ksenia

    2017-10-01

    The abandonment of churches and their adaptation for secular purposes is a current subject in Europe and worldwide. Most cases involve objects that were desacralized and then rebuilt as a whole object for alternative functions. Thus far, the merging of secular and sacred functions in one monumental Catholic church has not raised any issues. The paper describes the case of St. Catherine’s Church in Gdansk, Poland, where sacred function exists parallel to the new secular function being implemented. The study is based on the authentic, professional experience of the author. It describes the technical limitations arising from the need to ensure destinies for the optimal conditions of both sacred and secular function, while avoiding undesirable interference between them. The author further identifies architectural solutions most relevant to current requirements for protection of sacred zones in the church, for preservation of the monument, and for optimal function of a modern science museum. Significant design issues include: the inviolability of the sacred zone, preservation of the historical value of the monument, proper operation of new secular zones in compliance with contemporary standards of safety, performance of the assumed mission and profitability. The research indicates specific areas where the highest probability of collision exists between the sacred and profane and where technical problems are likely to occur.

  19. Density, Spatial Pattern and Relief Features of Sacred Sites in Northern Morocco

    Directory of Open Access Journals (Sweden)

    Holger Jäckle

    2013-02-01

    Full Text Available Sacred sites are of conservation value because of their spiritual meaning, as cultural heritage and as remnants of near-natural biotopes in landscapes strongly transformed by man. The vegetation of sacred sites in Morocco was studied recently. Information about their number, spatial pattern or relief position is fragmentary. However, these parameters are important to evaluate their role as refuge for organisms and their representativeness of potential natural vegetation. Therefore, density and spatial pattern of sacred sites on the Tangier Peninsula in NW Morocco were studied based on records on topographic maps and by ground check. Their relief position was examined calculating a logistic regression model based on site-presences and random pseudo-absences. A ground check showed that around 67% of the existing sacred sites are documented in the topographic maps. They occur in the whole study area but are agglomerated around settlements. Although sacred sites occur with preference at elevated sites they can be found in almost all relief positions, thus offering the potential of supporting different types of climax vegetation (climatic climax and pedoclimax. Because of their abundance (around 29 sacred sites / 100 km² and their distribution pattern they could serve as elements of a biotope network in degraded landscapes.

  20. The supernatural characters and powers of sacred trees in the Holy Land.

    Science.gov (United States)

    Dafni, Amots

    2007-02-25

    This article surveys the beliefs concerning the supernatural characteristics and powers of sacred trees in Israel; it is based on a field study as well as a survey of the literature and includes 118 interviews with Muslims and Druze. Both the Muslims and Druze in this study attribute supernatural dimensions to sacred trees which are directly related to ancient, deep-rooted pagan traditions. The Muslims attribute similar divine powers to sacred trees as they do to the graves of their saints; the graves and the trees are both considered to be the abode of the soul of a saint which is the source of their miraculous powers. Any violation of a sacred tree would be strictly punished while leaving the opportunity for atonement and forgiveness. The Druze, who believe in the transmigration of souls, have similar traditions concerning sacred trees but with a different religious background. In polytheistic religions the sacred grove/forest is a centre of the community's official worship; any violation of the trees is regarded as a threat to the well being of the community. Punishments may thus be collective. In the monotheistic world (including Christianity, Islam and Druze) the pagan worship of trees was converted into the worship/adoration of saints/prophets; it is not a part of the official religion but rather a personal act and the punishments are exerted only on the violating individual.

  1. Reconstructing sacred landscapes from soils-based records

    Science.gov (United States)

    Simpson, Ian; Gilliland, Krista; Coningham, Robin; Manuel, Mark; Davis, Christopher; Strickland, Keir; Acharya, Kosh; Hyland, Katherine; Bull, Ian; Kinnaird, Timothy; Sanderson, David

    2015-04-01

    From soils- and sediments- based records we reconstruct development of the sacred landscape at Lumbini UNESCO World Heritage Site in the central Nepalese Terai, the birthplace of Buddha, a world religion and now a major place of pilgrimage to its temple site. The Terai is a plain less than 100 m above sea level with incising rivers that originate in the Churia Hills and flow to the Ganges. Alluvial sediments on the Terai plain, originating as laterite soils within the hills, are characterised by a range of textural classes rich in iron oxides and manganese, with sandier sediments near water sources and finer textures near the distal ends of alluvial reaches. Our objectives are to establish a chronological framework for occupation, identify influences of alluvial environments on site occupation and determine the process of secular and sacred site formation within the World Heritage Site. A set of key stratigraphies are the basis for our analyses and are located in a palaeo-channel adjacent the temple site, within the temple site itself, and within the mound of the original Lumbini village. Optically stimulated luminescence (OSL) measurements of soils and sediments together with supporting single entity radiocarbon measurements provide robust chronological frameworks. Assessment of field properties, thin section micromorphology and organic biomarkers offer new insight into the intimate and complex relationships between natural, cultural and culturally mediated processes in landscape development. Integration of our findings allows a detailed narrative of cultural landscape development at Lumbini. The area was occupied from ca. 1,500 BC first of all by a transient community who used the area for product storage and who were subject to persistent flooding with periodic major flood events. Subsequent occupation deliberately raised a permanent village settlement above the level of flood events flooding and which had associated managed field cultivation. Village life was

  2. Sacred Symbols in Dimitrie Gavrilean’s Paintings

    Directory of Open Access Journals (Sweden)

    Emilian Adrian Gavrilean

    2016-05-01

    Full Text Available This year, on the 12th of July, there are four years since the passing away of the painter Dimitrie Gavrilean who, ,”through the unique impresion of his originality, early became an emblem of the school of painting of Iasi.” Born in Voroneţ, in 1942, Dimitrie Gavrilean attended ”Nicolae Grigorescu” Academy of Arts of Bucharest, being an eminent disciple of the master Corneliu Baba. The work in the creation workshop was combined with the vocation of teacher at the Faculty of Visual Arts and Design within ”G. Enescu” University of Arts of Iaşi, being a rector of this institution in the period 2000-2004. In the contemporary plastic landscape, the artistic work of the painter Dimitrie Gavrilean (1942-2012 from Iaşi occupies a special place. It mirrors the utmost of the autochthonous rural imaginary, the Romanian fundamental myths, the ancestral myths as well as the recently Christianized ones. His vision is specific to the Romanian Christianity and the Byzantine iconography. The language of Gavrilean’s paintings is one of symbols, by excellence. As Emil Staco rightfully observed, the entire work of art of master Gavrilean is ”a work of art of the symbols, of the metaphors rooted in the spirituality of the Romanian village of Bukovina, in the frescoes of Voroneţ and Humor Monasteries.” On the one hand, the painter did not content himself with illustrating only the ancestral mythical world by means of symbols with universal valences, but he got actively involved in the Christian valorization of mythical symbols. On the other hand, by integrating sacred symbols (the dove, the cock, winged characters, the wise old man, the architecture-church, the sun, Voroneţ blue of the Christian iconography of Byzantine tradition in paintings not necessarily religious, Dimitrie Gavrilean pushed his work of art to the border between sacred and profane, proving an extraordinary capacity of spiritualizing matter as well as of materializing the

  3. Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors.

    Science.gov (United States)

    Santhoshkumar, Thirunavukkarasu; Rahuman, Abdul Abdul; Rajakumar, Govindasamy; Marimuthu, Sampath; Bagavan, Asokan; Jayaseelan, Chidambaram; Zahir, Abdul Abduz; Elango, Gandhi; Kamaraj, Chinnaperumal

    2011-03-01

    The aim of this study was to investigate the larvicidal potential of the hexane, chloroform, ethyl acetate, acetone, methanol, and aqueous leaf extracts of Nelumbo nucifera Gaertn. (Nymphaeaceae) and synthesized silver nanoparticles using aqueous leaf extract against fourth instar larvae of Anopheles subpictus Grassi and Culex quinquefasciatus Say (Diptera: Culicidae). Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. The results recorded from UV-vis spectrum, scanning electron microscopy, X-ray diffraction, and Fourier transform infrared support the biosynthesis and characterization of silver nanoparticles. Larvae were exposed to varying concentrations of plant extracts and synthesized silver nanoparticles for 24 h. All extracts showed moderate larvicidal effects; however, the maximum efficacy was observed in crude methanol, aqueous, and synthesized silver nanoparticles against the larvae of A. subpictus (LC(50) = 8.89, 11.82, and 0.69 ppm; LC(90) = 28.65, 36.06, and 2.15 ppm) and against the larvae of C. quinquefasciatus (LC(50) = 9.51, 13.65, and 1.10 ppm; LC(90) = 28.13, 35.83, and 3.59 ppm), respectively. These results suggest that the leaf methanol, aqueous extracts of N. nucifera, and green synthesis of silver nanoparticles have the potential to be used as an ideal eco-friendly approach for the control of the A. subpictus and C. quinquefasciatus. This is the first report on the mosquito larvicidal activity of the plant extracts and synthesized nanoparticles.

  4. Étude de la diversité floristique par strates de quelques bois sacrés ...

    African Journals Online (AJOL)

    Étude de la diversité floristique par strates de quelques bois sacrés du Centre Bénin. ... Objectif : Cette étude vise à analyser la diversité floristique par strates de six bois sacrés au Centre Bénin. Méthodologie ... A cet effet ces bois sacrés font appel à un programme d'aménagement en vue de leur développement durable.

  5. Swimming Characteristics of Bioinspired Helical Microswimmers Based on Soft Lotus-Root Fibers

    Directory of Open Access Journals (Sweden)

    Jia Liu

    2017-11-01

    Full Text Available Various kinds of helical swimmers inspired by E. coli bacteria have been developed continually in many types of researches, but most of them are proposed by the rigid bodies. For the targeted drug delivery, the rigid body may hurt soft tissues of the working region with organs. Due to this problem, the biomedical applications of helical swimmers may be restricted. However, the helical microswimmers with the soft and deformable body are appropriate and highly adaptive in a confined environment. Thus, this paper presents a lotus-root-based helical microswimmer, which is fabricated by the fibers of lotus-root coated with magnetic nanoparticles to active under the magnetic fields. The helical microstructures are derived from the intrinsic biological structures of the fibers of the lotus-root. This paper aims to study the swimming characteristic of lotus-root-based microswimmers with deformable helical bodies. In the initial step under the uniform magnetic actuation, the helical microswimmers are bent lightly due to the heterogeneous distribution of the internal stress, and then they undergo a swimming motion which is a spindle-like rotation locomotion. Our experiments report that the microswimmers with soft bodies can locomote faster than those with rigid bodies. Moreover, we also find that the curvature of the shape decreases as a function of actuating field frequency which is related to the deformability of lotus-root fibers.

  6. Integrated Emergy and Economic Evaluation of Lotus-Root Production Systems on Reclaimed Wetlands Surrounding the Pearl River Estuary, China

    Science.gov (United States)

    Lotus (Neumbo nucifera, Gaertn) is the most important aquatic vegetable in China, with a cultivation history of over 3000 years. The emergy, energy, material, and money flows of three lotus root cultivation modes in Wanqingsha, Nansha District, Guangzhou, China were examined usin...

  7. Mourning Mandela: sacred drama and digital visuality in Cape Town

    Directory of Open Access Journals (Sweden)

    Paula Uimonen

    2015-12-01

    Full Text Available The world united in unprecedented ways in mourning the global icon Nelson Mandela, an emotionally charged historical event in which digital visuality played an influential role. The memorial service for Nelson Mandela on Tuesday, 10 December 2013, gathered dignitaries and celebrities from around the world at the First National Bank Stadium in Johannesburg, to mourn the passing of Madiba and to celebrate his life work. At the Grand Parade in Cape Town, the event was broadcast on large public screens, followed by live music performances and narrowcast interaction with the audience. Building on recent research on public screens during global media events, this article addresses the mediated mourning rituals at the Grand Parade in terms of a sacred drama. Focusing on social relationality, the article discusses how digital visuality mediated a sense of global communitas, thus momentarily overcoming historical frictions between the global north and the global south, while expanding the fame of Madiba. Paying attention to the public display of visual memory objects and the emotional agency of images, it argues that digital visuality mediated social frictions between the living and the dead, while recasting a historical subject as a historical object. The article further discusses how digital visuality mediated cultural frictions of apartheid and xenophobia, through the positioning of Mandela in the pantheon of Pan-African icons, thus underlining the African origin of this global icon. The analysis is based on ethnographic observations and experiences in Cape Town.

  8. Tchen's Sacred Isolation—Prelude to Malraux's Fraternal Humanism

    Directory of Open Access Journals (Sweden)

    Roch C. Smith

    1982-09-01

    Full Text Available While Malraux's life-long quest was to seek new values in man's perennial and shared struggle against an overwhelming fate, his early protagonist, particularly the assassin, turns to destruction and terrorism in a frenzied search for absolutes. This attempt to identify with the very fatality that has the power to destroy him is especially developed in Tchen, who embodies a despairing fascination with totalistic nihilism that Malraux must overcome in his search for a new notion of man. Tchen's initiation to murder in La Condition humaine marks a transgression of a taboo that thrusts him into what Georges Bataille calls the realm of the "sacred." His attempt to reconcile life and death by identifying with his victim irredeemably isolates Tchen from other, uninitiated men. Transformed by murder, he leaves the reality of revolution for the inhuman world of cosmic existence and individual death. Seeking to escape the human condition, he becomes obsessed with killing Chang-Kai-shek in order to kill himself and thereby "possess" his fate. But the illusion of such an escape dies with Tchen. Even his admiring disciples repudiate his nihilistic temptation as Malraux begins to seek in human fraternity the foundations of a new humanism.

  9. The sacred weapon: bow and arrow combat in Iran

    Directory of Open Access Journals (Sweden)

    Manouchehr Moshtagh Khorasani

    2012-07-01

    Full Text Available The following article presents the development of the bow and arrow, and its important role in the history of Iran. The bow always played an important role not only on the battlefield, but also in hunting. It was also considered as a sacred weapon and additionally a royal symbol. Bow and arrow were considered as a superior weapon in comparison with other types of weapons because one could fight with them at a safer distance as one offered by swords, maces and axes. The first part of the article presents a short history of the bow in Iran. Based on historical Persian manuscripts, the next part explains the structure of the composite bow and the materials used for making it. The third part describes some types of bows based on the material, place of production, the usage, and bow type based on the length of the bow and the arrows. The following part talks about different types of arrows based on morphology of arrowheads, the type of plume/feather, the material of the shaft, the material of the arrowhead, the length of arrows, the target of arrows, the place of production of arrowheads and terms for describing its different features of an arrowhead. Then, the article talks about different types of thumb rings, bowstrings, quivers and bow cases and arrow guides for shooting short arrows. The next part discusses different principles of archery as explained in Persian manuscripts. Finally the article describes different archery targets.

  10. Crossing the Sacred/Secular Divide; Unraveling Turkish Identities

    Directory of Open Access Journals (Sweden)

    Morrow Lisa

    2016-06-01

    Full Text Available This paper unpacks the ideas in the poem “Pull Down My Statues” by Süleyman Apaydın, to examine some common descriptors in use about modern Turkey. Taking his inspiration from Mustafa Kemal Atatürk, the founder of the modern Turkish republic, Apaydın ponders the success of Atatürk’s vision, based on the idea of a secular/sacred divide. Combining this with the way travel in Turkey is heavily promoted using the same themes, I explore how this divide, with its underlying connotations of West versus East and modernity versus tradition (as found in Turkey’s Ottoman past, is applied to Turkish identity. Turks are commonly portrayed as a homogenous people only differentiated by their degree of religiosity, but I argue that this analysis is too simplistic. Turkish identity has never been based on a single clear cut model, and this is becoming obvious as more traditional Islamic ways of life are being reworked by new forms of Islam based on capitalism. Consequently, although it is important to acknowledge Turkey’s past, looking to history for a way to steer through the complexities of the present is no longer useful or even relevant.

  11. Rituals, ceremonies and customs related to sacred trees with a special reference to the Middle East

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    Dafni Amots

    2007-07-01

    Full Text Available Abstract Tree worship is very common worldwide. This field study surveys the ceremonies and customs related to sacred trees in present-day Israel; it includes the results of interviews with 98 informants in thirty-one Arab, Bedouin, and Druze villages in the Galilee. The main results are: 1. Sacred trees were treated as another kind of sacred entity with all their metaphysical as well as physical manifestations. 2. There is not even one ceremony or custom that is peculiar only to a sacred tree and is not performed in other sacred places (such as a saint's grave or a mosque. 3. Few customs, such as: quarrel settling (= Sulkha, leaving objects to absorb the divine blessing and leaving objects for charity seem to be characteristic of this region, only. 4. In modern times, sacred trees were never recorded, in Israel, as centres for official religious ceremonies including sacrifices, nor as places for the performing of rites of passage. 5. There is some variation among the different ethnic groups: Kissing trees and worshipping them is more common among the Druze although carrying out burials under the tree, leaving water and rain-making ceremonies under them have not been recorded in this group. Passing judgments under the tree is more typical of the Bedouin in which the sacred trees were commonly used as a public social centre. Most of the customs surveyed here are known from other parts of the world. The differences between Muslims and Druze are related to the latter's belief in the transmigration of souls.

  12. Li Yujie and the Rebranding of the White Lotus Movement

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    David Ownby

    2017-09-01

    Full Text Available Li Yujie (1900–1994 was a walking contradiction: a student leader of the Shanghai May Fourth movement and a Guomindang member and technocrat in the Nanjing government, but also a cadre in Xiao Changming’s redemptive society—the Heavenly Virtues Teachings—and eventually the founder of two redemptive societies in his own right (the Heaven and Man Teachings and the Heavenly Emperor Teachings. Through a biographical study of Li Yujie, this article examines the complex appeal of redemptive societies to parts of the educated elite during China’s Republican period. The author focuses particularly on the period between 1937 and 1945, when Li retired to the sacred mountain of Huashan. There, with the help of Huang Zhenxia, a self-taught intellectual also employed by the Guomindang, Li sought to modernize the “White Lotus” teachings that he had received from his master by incorporating scientific insights received via spirit writing. Li believed that he was creating a new religion more adapted to the twentieth century. Both the texts produced on Huashan and the military and political elite that were attracted to these texts allow us to raise new questions about secularism and religion, traditional beliefs and science in the context of Republican-period China, thereby suggesting that the conflict between the modernizing state and traditional religious culture was not always as stark as we have believed it to be.

  13. Anti-spasmodic effects of Zizyphus lotus (L.) Desf. extracts on isolated rat duodenum.

    Science.gov (United States)

    Borgi, Wahida; Chouchane, Nabil

    2009-12-10

    The present study deals with the anti-spasmodic activity of the aqueous and the methanolic extracts of the leaves and the root barks of Zizyphus lotus (L.) Desf. on male rats. This activity was assessed on contractions of isolated rat duodenum, induced by acetylcholine, KCl, and BaCl(2) and compared with the effect of atropine and papaverine. Both extracts of leaves and root barks caused significant relaxation of spontaneous contractions and produced a concentration-dependent inhibition (PZizyphus lotus extracts contain anti-spasmodic constituents mediating their effect through cholinergic receptors and blockade Ca(2+) influx. This could explain the traditional use of Zizyphus lotus in the treatment of the intestinal diseases.

  14. Adsorption characteristics of methylene blue onto agricultural wastes lotus leaf in bath and column modes.

    Science.gov (United States)

    Han, Xiuli; Wang, Wei; Ma, Xiaojian

    2011-01-01

    The adsorption potential of lotus leaf to remove methylene blue (MB) from aqueous solution was investigated in batch and fixed-bed column experiments. Langmuir, Freundlich, Temkin and Koble-Corrigan isotherm models were employed to discuss the adsorption behavior. The results of analysis indicated that the equilibrium data were perfectly represented by Temkin isotherm and the Langmuir saturation adsorption capacity of lotus leaf was found to be 239.6 mg g(-1) at 303 K. In fixed-bed column experiments, the effects of flow rate, influent concentration and bed height on the breakthrough characteristics of adsorption were discussed. The Thomas and the bed-depth/service time (BDST) models were applied to the column experimental data to determine the characteristic parameters of the column adsorption. The two models were found to be suitable to describe the dynamic behavior of MB adsorbed onto the lotus leaf powder column.

  15. The Proteome of Seed Development in the Model Legume Lotus japonicus

    DEFF Research Database (Denmark)

    Dam, Svend; Laursen, Brian S.; Ornfelt, Jane H.

    2009-01-01

    We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic...... three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea......, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique...

  16. Fabrication of Lotus-Type Porous Aluminum through Thermal Decomposition Method

    Science.gov (United States)

    Kim, S. Y.; Park, J. S.; Nakajima, H.

    2009-04-01

    Lotus-type porous aluminum with cylindrical pores was fabricated by unidirectional solidification through thermal decomposition of calcium hydroxide, sodium bicarbonate, or titanium hydride. The pore-forming gas decomposed from calcium hydroxide, sodium bicarbonate, and titanium hydride is identified as hydrogen. The elongated pores are evolved due to the solubility gap between liquid and solid when the melt dissolving hydrogen is solidified unidirectionally. The porosity of lotus aluminum is as high as 20 pct despite the type of the compounds. The pore size decreases and the pore density increases with increasing amount of calcium hydroxide, which is explained by an increase in the number of pore nucleation sites. The porosity and pore size in lotus aluminum fabricated using calcium hydroxide decrease with increasing argon pressure, which is explained by Boyle’s law. It is suggested that this fabrication method is simple and safe, which makes it superior to the conventional technique using high-pressure hydrogen gas.

  17. The Proteome of Seed Development in the Model Legume Lotus japonicus1[C][W

    Science.gov (United States)

    Dam, Svend; Laursen, Brian S.; Ørnfelt, Jane H.; Jochimsen, Bjarne; Stærfeldt, Hans Henrik; Friis, Carsten; Nielsen, Kasper; Goffard, Nicolas; Besenbacher, Søren; Krusell, Lene; Sato, Shusei; Tabata, Satoshi; Thøgersen, Ida B.; Enghild, Jan J.; Stougaard, Jens

    2009-01-01

    We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea. In a first attempt to determine the seed proteome, both a two-dimensional polyacrylamide gel electrophoresis approach and a gel-based liquid chromatography-mass spectrometry approach were used. Globulins were analyzed by two-dimensional polyacrylamide gel electrophoresis, and five legumins, LLP1 to LLP5, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases, respectively. All of the proteome data, including the experimental data and mass spectrometry spectra peaks, were collected in a database that is available to the scientific community via a Web interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry, and regulation of seed development in Lotus. Together with a new Web interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family. PMID:19129418

  18. The proteome of seed development in the model legume Lotus japonicus.

    Science.gov (United States)

    Dam, Svend; Laursen, Brian S; Ornfelt, Jane H; Jochimsen, Bjarne; Staerfeldt, Hans Henrik; Friis, Carsten; Nielsen, Kasper; Goffard, Nicolas; Besenbacher, Søren; Krusell, Lene; Sato, Shusei; Tabata, Satoshi; Thøgersen, Ida B; Enghild, Jan J; Stougaard, Jens

    2009-03-01

    We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea. In a first attempt to determine the seed proteome, both a two-dimensional polyacrylamide gel electrophoresis approach and a gel-based liquid chromatography-mass spectrometry approach were used. Globulins were analyzed by two-dimensional polyacrylamide gel electrophoresis, and five legumins, LLP1 to LLP5, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases, respectively. All of the proteome data, including the experimental data and mass spectrometry spectra peaks, were collected in a database that is available to the scientific community via a Web interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry, and regulation of seed development in Lotus. Together with a new Web interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.

  19. Sediment Dynamics in a Vegetated Tidally Influenced Interdistributary Island: Wax Lake, Louisiana

    Science.gov (United States)

    2017-07-01

    interior. The emergence of American lotus (Nelumbo lutea) in summer forms a dense canopy over the island reducing wave and current energy, increasing the...environmental concerns of this century (Nicholls and Cazenave 2010). An encroaching sea increases the likelihood of flooding and storm inundation, imposing...aquatic vegetation is the American lotus (Nelumbo lutea). Nelumbo lutea is an emergent, aquatic plant common to the southeastern United States and grows

  20. Are Brands Postmodern Relics? Taking a Closer Look at New Sacred Objects

    Directory of Open Access Journals (Sweden)

    Stéphane DUFOUR

    2011-01-01

    Full Text Available Ever since the Church lost its monopoly on the sacred, no longer able to solely determine its form or contents, the social sphere has gradually taken over this value, applying it to new human and social objects. As a result, the modes of expression of the sacred have multiplied, along with the subjective and intimate experiences of modern individualism. Among the vast number of potential manifestations of this value, to which almost everything now seems to aspire, this paper will concentrate on commercial brands as vectors of meaning, with the hypothesis that some of them seek to position themselves, in postmodern society, as new figures of the sacred. This area of study is close to that of the sociologist Adam Arvidsson, when he describes brands as religious objects. If brands are less interested in selling products than in creating an affective experience, Arvidsson assimilates them to modern relics. However, this paper goes beyond metaphors, to examine the rhetorical strategies (discourse, rituals, representations, imagery through which brands construct meaning around sacred objects. Situated between a branch of marketing which concentrates on sacralising commercial products, and a theory popular in the English-speaking world, which has illustrated how the media work to sacralise products and brands, this paper uses a communicational approach to analyse the construction of meaning, by brands looking to make themselves (appear sacred.

  1. Phenolic Profiles and Antioxidant Activity of Lotus Root Varieties

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    Yang Yi

    2016-06-01

    Full Text Available Lotus root attracts increasing attention mainly because of its phenolic compounds known as natural antioxidants. Its thirteen varieties were systematically analyzed on the content, distribution, composition and antioxidant activity of phenolic compounds for a better understanding of this aquatic vegetable. The respective mean contents of total phenolics in their flesh, peel and nodes were 1.81, 4.30 and 7.35 mg gallic acid equivalents (GAE/g fresh weight (FW, and those of total flavonoids were 3.35, 7.69 and 15.58 mg rutin equivalents/g FW. The phenolic composition determined by a high-performance liquid chromatography method varied significantly among varieties and parts. The phenolics of flesh were mainly composed of gallocatechin and catechin; those of peel and node were mainly composed of gallocatechin, gallic acid, catechin and epicatechin. The antioxidant activities of phenolic extracts in increasing order were flesh, peel and node; their mean concentrations for 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical were 46.00, 26.43 and 21.72 µg GAE/mL, and their mean values representing ferric reducing antioxidant power were 75.91, 87.66 and 100.43 µg Trolox equivalents/100 µg GAE, respectively. “Zoumayang”, “Baheou”, “No. 5 elian” and “Guixi Fuou” were the hierarchically clustered varieties with relatively higher phenolic content and stronger antioxidant activity as compared with the others. Especially, their nodes and peels are promising sources of antioxidants for human nutrition.

  2. Pedobacter lotistagni sp. nov. isolated from lotus pond water.

    Science.gov (United States)

    Singh, Hina; Du, Juan; Ngo, Hien T T; Kim, Ki-Young; Yi, Tae-Hoo

    2015-04-01

    A light-yellow coloured, Gram-stain negative, rod-shaped, aerobic, non-motile bacterium, designated THG-DN6.8(T), was isolated from a lotus pond near Donghaksa temple in Daejeon, South Korea. The strain was found to grow well on nutrient agar, optimally at pH 6.0-7.5, at temperature 25-28 °C and in the presence of 0.5 % NaCl. Based on 16S rRNA gene sequence analysis, strain THG-DN6.8(T) was found to share the highest sequence similarity with Pedobacter koreensis KCTC 12536(T), followed by Pedobacter glacialis CCTCC AB 2012941(T), Pedobacter kyungheensis KACC 16221(T), Pedobacter caeni LMG 22862(T), Pedobacter insulae KCTC 12820(T) and Pedobacter boryungensis KCTC 23344 (T). The DNA G+C content was determined to be 36.8 mol%. In DNA-DNA hybridization tests, the DNA relatedness between strain THG-DN6.8(T) and its closest phylogenetic neighbour P. koreensis was found to be below 10 %. The predominant isoprenoid quinone was identified as menaquinone MK-7 and the major polar lipid as phosphatidylethanolamine. The major fatty acids of strain THG-DN6.8(T) were identified as iso-C15:0, C16:0, C18:0 and C16:1 ω6c and/or C16:1 ω7c (summed feature 3). On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain THG-DN6.8(T) is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter lotistagni sp. nov. is proposed. The type strain is THG-DN6.8(T) (= KCTC 42229(T) = JCM 30354(T)).

  3. Novosphingobium lotistagni sp. nov., isolated from a lotus pond.

    Science.gov (United States)

    Ngo, Hien T T; Trinh, Huan; Kim, Jung-Hee; Yang, Jung-Eun; Won, Kyung-Hwa; Kim, Ju-Han; Kook, MooChang; Yi, Tae-Hoo

    2016-11-01

    A Gram-staining-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.20T, was isolated from a lotus pond near Donghaksa temple in Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN6.20T was found to be most closely related to Novosphingobium rosa IFO 15208T (97.6 % sequence similarity), Novosphingobium sediminicola HU1-AH51T (97.5 %) and Novosphingobium barchaimii LL02T (96.9 %). The DNA-DNA relatedness between strain THG-DN6.20T and its phylogenetically closest neighbours was below 60.0 %. The respiratory quinone and polyamine detected in strain THG-DN6.20T were ubiquinone Q-10 and spermidine, respectively. The DNA G+C content was 63.1 mol%. The major polar lipids were found to be phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipid and phosphatidylcholine. The major fatty acids were identified as C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C14 : 0 2-OH. These data supported the affiliation of strain THG-DN6.20T to the genus Novosphingobium. Strain THG-DN6.20T could be distinguished from related species of the genus Novosphingobium by physiological and biochemical characteristics. Therefore, the novel isolate represents a novel species, for which the name Novosphingobium lotistagni sp. nov. is proposed, with THG-DN6.20T as the type strain (=KACC 18541T=CCTCC AB 2015354T).

  4. Desmond and Moore’s Darwin’s Sacred Cause

    Directory of Open Access Journals (Sweden)

    Allen Esterson

    2013-04-01

    Full Text Available In their book Darwin’s Sacred Cause (2009 Adrian Desmond and James Moore purport to demonstrate that they have provided an original explanation for the inspiration behind Darwin’s determined pursuit of an explanatory theory for the transformation of species of which he became convinced as a result of his experiences during the Beagle voyage of 1831 to 1836. This, they argue, was the “moral passion” that was evoked by his encountering the horrors of slavery during the periods he was able to disembark to explore areas of South America in the years 1832 to 1835. In short, they provide what they describe as “the untold story of how Darwin’s abhorrence of slavery led to our modern understanding of evolution.” This article explores the means by which the authors seek to persuade readers of the validity of their thesis, and concludes that far from providing compelling evidence, by providing a mass of historically interesting material relating to slavery that is actually tangential to their case, they obscure the fact that they fail to accomplish their aim. There is nothing in their account of events that provides a reason for preferring their explanation for Darwin’s devotion to understanding the processes involved in the transformation of species to the known historical facts of Darwin’s early zeal for natural science, exhibited in his childhood exploits with beetles and his more organized scientific activities when he was a student at Cambridge, enabled to come to fruition by his scientific activities during the voyage of the Beagle.

  5. Condensed Tannin Concentrations of Three Lotus Species Grown in Different Environments Contenido de Taninos Condensados en Germoplasma de Tres Especies del Género Lotus en Diferentes Ambientes

    Directory of Open Access Journals (Sweden)

    Hernán Acuña

    2008-03-01

    Full Text Available Condensed tannins (CT present in Lotus spp., depending on their concentration in the plant, can prevent bloating and improve protein absorption in ruminants. With the objective to know the variability of this characteristic, the CT concentration (% dry matter basis was determined by the butanol-HCl procedure in 13 cultivars of Lotus corniculatus L. (Lc introduced from North and South America and Oceania, 11 accessions of Lotus tenuis Waldst. & Kit. ex Willd. (Lt, and 22 accessions of Lotus uliginosus Schkuhr (Lu collected in Chile, from 32° to 38° S and 36° to 46° S, respectively. The field experiments, with two randomized complete blocks, were established in autumn 1998, in five different environments in the central-southern zone of Chile (clay soil, sandy soil, and three volcanic soils with different levels of water availability. The herbage sampling was conducted in spring-summer 1999-2000, when the plants were in full flowering. Significant differences between cultivars or accessions were found within each site, in total CT and/or in extractable or protein or fiber bound fractions of CT concentrations. The Lc and Lu genotypes showed high variability, with a range of 4.7 to 8.7% and 6.3 to 11.0%, and means of 6.1 and 8.2%, respectively, of total CT. The Lt general mean was 4.8%, considerably higher than the figures reported in the literature, and had low variability. This information will contribute to select genotypes for the improvement of this species by selection and breedingLos taninos condensados (TC presentes en Lotus spp ., dependiendo de su concentración en la planta, pueden evitar el meteorismo y favorecer la absorción de proteína en rumiantes. Con el objetivo de conocer la variabilidad de esta característica se determinó por el método del butanol-HCl la concentración (% base materia seca de TC en 13 cultivares de Lotus corniculatus L. (Lc introducidos de Norteamérica, Sudamérica y Oceanía, 11 accesiones de Lotus

  6. Anti-angiogenic effect of Nelumbo nucifera leaf extracts in human umbilical vein endothelial cells with antioxidant potential.

    Directory of Open Access Journals (Sweden)

    Jong Suk Lee

    Full Text Available Nelumbo nucifera Gaertn (Nymphaeaceae has long been used as a traditional herb in Chinese, Japanese, Indian, and Korean medicinal practices since prehistoric times and flourishes today as the primary form of medicine. This study reports for the first time the potent ability of N. nucifera leaf extracts to inhibit vascular endothelial growth factor (VEGF-induced angiogenesis in vitro and in vivo, as well as their antioxidant efficacy in various scavenging models and an analysis of their chemical composition. In vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM model using fertilized chicken eggs, in human umbilical vein endothelial cells (HUVECs by using cell viability, cell proliferation and tube formation assays, and by determining intracellular reactive oxygen species (ROS in vitro. The antioxidant efficacy of N. nucifera leaf extracts was determined in various scavenging models, including total phenolic and flavonoid content. The chemical composition of N. nucifera leaf extracts was determined by GC-MS analysis, which revealed the presence of different phytochemicals. The IC50 values for the DPPH radical scavenging activities of water and methanol extracts were found to be 1699.47 and 514.36 μg ml(-1, and their total phenolic and flavonoid contents were 85.01 ± 2.32 and 147.63 ± 2.23 mg GAE g dry mass(-1 and 35.38 ± 1.32 and 41.86 ± 1.07 mg QA g dry mass(-1, respectively. N. nucifera leaf extracts (10-100 μg ml(-1 exhibited significant dose-dependent inhibition of VEGF-induced angiogenesis, as well as VEGF-induced proliferation and tube formation in HUVECs. In this study, N. nucifera leaf extracts displayed potent antioxidant and inhibitory effects on VEGF-induced angiogenesis. N. nucifera exerted an inhibitory effect on VEGF-induced proliferation and tube formation, as well as CAM angiogenesis in vivo. Moreover, N. nucifera leaf extracts significantly blocked VEGF-induced ROS production in HUVECs

  7. Anti-angiogenic effect of Nelumbo nucifera leaf extracts in human umbilical vein endothelial cells with antioxidant potential.

    Science.gov (United States)

    Lee, Jong Suk; Shukla, Shruti; Kim, Jung-Ae; Kim, Myunghee

    2015-01-01

    Nelumbo nucifera Gaertn (Nymphaeaceae) has long been used as a traditional herb in Chinese, Japanese, Indian, and Korean medicinal practices since prehistoric times and flourishes today as the primary form of medicine. This study reports for the first time the potent ability of N. nucifera leaf extracts to inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis in vitro and in vivo, as well as their antioxidant efficacy in various scavenging models and an analysis of their chemical composition. In vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM) model using fertilized chicken eggs, in human umbilical vein endothelial cells (HUVECs) by using cell viability, cell proliferation and tube formation assays, and by determining intracellular reactive oxygen species (ROS) in vitro. The antioxidant efficacy of N. nucifera leaf extracts was determined in various scavenging models, including total phenolic and flavonoid content. The chemical composition of N. nucifera leaf extracts was determined by GC-MS analysis, which revealed the presence of different phytochemicals. The IC50 values for the DPPH radical scavenging activities of water and methanol extracts were found to be 1699.47 and 514.36 μg ml(-1), and their total phenolic and flavonoid contents were 85.01 ± 2.32 and 147.63 ± 2.23 mg GAE g dry mass(-1) and 35.38 ± 1.32 and 41.86 ± 1.07 mg QA g dry mass(-1), respectively. N. nucifera leaf extracts (10-100 μg ml(-1)) exhibited significant dose-dependent inhibition of VEGF-induced angiogenesis, as well as VEGF-induced proliferation and tube formation in HUVECs. In this study, N. nucifera leaf extracts displayed potent antioxidant and inhibitory effects on VEGF-induced angiogenesis. N. nucifera exerted an inhibitory effect on VEGF-induced proliferation and tube formation, as well as CAM angiogenesis in vivo. Moreover, N. nucifera leaf extracts significantly blocked VEGF-induced ROS production in HUVECs, confirming

  8. Behind the sacred tree: local people and their natural resources sustain-ability

    Directory of Open Access Journals (Sweden)

    Mohammad Royyani

    2014-12-01

    Full Text Available Local communities have their own means of maintaining their traditional know-ledge and sustaining the production system of natural resources by designating the resources as sacred. Without the state’s influence, local people have their own strategies to conserve the environment and resources, in ways which are more effective than those enforced by the state. A study done through interview, participatory observation, and ethnographic methods revealed that local people recognized two models of natural resources conservation. The first model is the designation of forests as sacred site, aiming at maintaining the sustainability of ecosystem and the second model refers to adoption of species as a sacred entity to sustain production system. Dynamic processes are operating in the sacredness of both forest and species. 

  9. The nature and workings of sacred intellect from the perspective of Avicenna

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    Einollah Khademi

    2017-08-01

    Full Text Available Sacred intellect in Avicenna, a degree of rationality in which the highest form of communication is done with the intellectual world. Reasonable time to the times of human rationality is achieved without difficulty, pain and time, only be achieved by applying guess. The owners of Avicenna's top intellects in human beings are sacred and highest prophet for them. These people are the first intelligible human teachers perceive first and put it to other people. . Bu-Ali about how to communicate the power of the active intellect and innate or acquired its own works differently to comment the funds are somehow retractable. To prove his philosophical question of the nature and functions of the sacred intellect as much existence of intellect, perception as well as all forms of The Holy Quran verses seeks help.

  10. An ethnobotanical study of medicinal plants used in sacred groves of Kumaon Himalaya, Uttarakhand, India.

    Science.gov (United States)

    Singh, Harsh; Husain, Tariq; Agnihotri, Priyanka; Pande, P C; Khatoon, Sayyada

    2014-05-28

    International organizations recognize the importance of sacred groves and place them into the context of sustainable development and also emphasize to conserve biodiversity through protection of sacred groves and sties. The significance of medicinal plants from Himalayan region is well known to the world. Therefore, present study was conducted in identified sacred groves of Kumaon Himalaya to investigate and document the utilization of medicinal plants by various local communities and tribal people. The study was conducted during 2008-2011 in four seasons of the year. Information was collected from 70 locals from different sacred groves by using free listing interviews with randomly selected informants and semi-structured questionnaires; plant specimens were collected, identified and deposited at the CSIR-NBRI herbarium (LWG), Lucknow, India. Seven sacred groves viz., Dhwaj, Haat Kali, Hokra, Malay Nath, Nakuleshwar, Narayan Swami Ashram and Patal Bhuvneshwar were identified from the Pithoragarh district of Kumaon Himalaya. 89 medicinal plants belonging to 52 families and 77 genera of which, 2 are lichens, 4 are pteridophytes, 3 are gymnosperms and remaining 80 plant species are angiosperms. 6 plant species are reported with new therapeutic uses for the first time in this paper. Highest informant׳s consensus factor value was found in liver disorder (0.55) and least by body pains (0.23). 55 ethnomedicinal plants are showing 100% fidelity level against various diseases. Sacred groves in Kumaon region of Indian Himalaya are rich sources and best repository of ethno-medicinally important plants with many rare, endangered and threatened species. It is an excellent example of unique traditional way of in situ conservation of different plant species. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  11. Sacred Grove of Punyagiri Hill, Vizianagaram District, AP, India: Ecological And Sociological Study

    Directory of Open Access Journals (Sweden)

    D. Srinivasa Rao

    2015-02-01

    Full Text Available  Sacred groves are important repositories of rare endangered endemic plants and floral diversity that have been conserved by the local people or communities in a sustainable manner. Sri Umakotilingeswara Swami Temple is a famous Siva Kshetra located in Vizianagaram district of the Andhra Pradesh, India. This holy shrine is situated in the Punyagiri hills, 4 kms away from the Srungavarapu Kota and 62 kms away from the Visakhapatnam (a coastal city of Andhra Pradesh. Two study sites were selected, one was in the sacred grove region another was in forest region. Line transects were used for collection of data on species richness and diversity of the flora. Density and basal area were more in the sacred grove region than the nearby forest region. Some important medicinal and valuable plants were reported in this region, plants such as Saraca asoca (Roxb., Diospyros peregryna (L., Sterculia urens (Roxb., Cleistanthus collinus (Roxb., Ficus relisiosa (L., Strublus aspera (L., Chloroxylon swietenia (L., Firmiana colorata (Roxb., Albizia odoratissima (L., Dalbergia paniculata (Roxb., Dalbergia sisso (Roxb., Azadiricta indica A. Juss., Diospyros chloroxylon (L., Holoptelea integrifolia (Roxb., Mangifera indica (L., Eucalyptus globulesv(Labelle, Bombax ceieba (L., Aegle marmelos, Ailanthus excelsa and some important pteridophytes and Bryophytes were reported in the sacred forest of the Punyagiri hills. Extension of the temple buildings and other developmental activities may be threat to the sacred groves. Invasion by the exotic weeds may further degrade the structure and composition of the sacred forests as a whole.DOI: http://dx.doi.org/10.3126/ije.v4i1.12176 International Journal of Environment Volume-4, Issue-1, Dec-Feb 2014/15, page: 30-47

  12. Sacred values in the Israeli-Palestinian conflict: resistance to social influence, temporal discounting, and exit strategies.

    Science.gov (United States)

    Sheikh, Hammad; Ginges, Jeremy; Atran, Scott

    2013-09-01

    Conflicts over sacred values may be particularly difficult to resolve. Because sacred values are nonfungible with material values, standard attempts to negotiate, such as offering material incentives to compromise, often backfire, increasing moral outrage and support for violent action. We present studies with Palestinians in the West Bank and Gaza demonstrating three other ways sacred values may make conflict more intractable, focusing on what we call devoted actors, people who regard issues central to the Israel-Palestine conflict as sacred values. We show that devoted actors (1) were less amenable to social influence, (2) perceived conflict-related events in the past as well as expected events in the future to be temporally closer, and (3) were blind to individual opportunities to escape the conflict. These results suggest that sacred values may affect decision making in a number of ways, which, when combined, contribute to common defense and continuation of conflict. © 2013 New York Academy of Sciences.

  13. Dancing the Numinous: Sacred and Spiritual Techniques of Contemporary American Belly Dancers

    Directory of Open Access Journals (Sweden)

    Jeana Jorgensen

    2013-01-01

    Full Text Available In this paper, I explore how contemporary American practitioners of belly dance (as Middle Eastern dance and its many varieties are often called in the English-speaking world conceptualize not only the spiritual dimensions of their dance, but also how the very notion of performance affects sacred and spiritual dance practices. Drawing on interviews with this community, I describe the techniques of sacred and spiritual belly dancers, how these dancers theorize performance, and how the conflicts inherent to patriarchal mind-body dualism are resolved in these practices. My purpose here is twofold: to document an emergent dance tradition and to analyze its meanings in the relevant social context.

  14. 'They Never Dance': The Choreography of Le Sacre du Printemps, 1913

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    Hanna Järvinen

    2013-12-01

    Full Text Available In this text, I discuss Vaslav Nijinsky's choreography of Le Sacre du Printemps (henceforth Sacre as it appears in the light of primary source materials from 1913. By focusing on the unique challenges Nijinsky posed to his dancers in terms of movement style and composition, I contest many of the frequently-heard claims made about this work, particularly its danced component, and argue that Nijinsky's choreographic ideas challenged both dancers and critics by questioning the ontological qualities of (art dance in contemporary discourse.

  15. Once Upon a Toxic Sanctuary: Partnering to Restore and Reclaim a Dakota Sacred Site

    Directory of Open Access Journals (Sweden)

    Roxanne Gould

    2017-10-01

    Full Text Available In this article, we examine the role of partnerships as they relate to the destruction and reconstruction of Wakaŋ Tipi and Indian Mounds Park as a Dakota sacred feminine, origin, birth site through a theoretical lens of critical Indigenous pedagogy of place (Trinidad, 2016 and partnership studies (Eisler, 2005. We discuss the deep historical, social, psychological, and cultural relationship the Dakota have to this sacred site and the challenge of partnering with non-Dakota entities to restore Wakaŋ Tipi/Indian Mounds Park from a toxic waste dump to a spiritual sanctuary.

  16. Dancing the Numinous: Sacred and Spiritual Techniques of Contemporary American Belly Dancers

    Directory of Open Access Journals (Sweden)

    Jeana Jorgensen

    2012-12-01

    Full Text Available In this paper, I explore how contemporary American practitioners of belly dance (as Middle Eastern dance and its many varieties are often called in the English-speaking world conceptualize not only the spiritual dimensions of their dance, but also how the very notion of performance affects sacred and spiritual dance practices. Drawing on interviews with this community, I describe the techniques of sacred and spiritual belly dancers, how these dancers theorize performance, and how the conflicts inherent to patriarchal mind-body dualism are resolved in these practices. My purpose here is twofold: to document an emergent dance tradition and to analyze its meanings in the relevant social context.

  17. Infection of Lotus japonicus Roots by Mesorhizobium loti

    DEFF Research Database (Denmark)

    Markmann, Katharina; Radutoiu, Elena Simona; Stougaard, Jens

    2012-01-01

    Like the two important crop legumes soybean and common bean, the model legume Lotus japonicus develops determinate root nodules. L. japonicus is normally infected through root hair infection threads in a process closely synchronised with the progressing primordial cell divisions and organ...

  18. Expression of a complete soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus

    DEFF Research Database (Denmark)

    Stougaard, J; Petersen, T E; Marcker, K A

    1987-01-01

    The complete soybean leghemoglobin lbc(3) gene was transferred into the legume Lotus corniculatus using an Agrobacterium rhizogenes vector system. Organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with Rhizobium loti...

  19. N-glycan maturation mutants in Lotus japonicus for basic and applied glycoprotein research

    DEFF Research Database (Denmark)

    Pedersen, Carina T; Loke, Ian; Lorentzen, Andrea

    2017-01-01

    Studies of protein N-glycosylation are important for answering fundamental questions on the diverse functions of glycoproteins in plant growth and development. Here we generated and characterised a comprehensive collection of Lotus japonicusLORE1 insertion mutants, each lacking the activity of on...

  20. In vitro corrosion resistance of Lotus-type porous Ni-free stainless steels.

    Science.gov (United States)

    Alvarez, Kelly; Hyun, Soong-Keun; Fujimoto, Shinji; Nakajima, Hideo

    2008-11-01

    The corrosion behavior of three kinds of austenitic high nitrogen Lotus-type porous Ni-free stainless steels was examined in acellular simulated body fluid solutions and compared with type AISI 316L stainless steel. The corrosion resistance was evaluated by electrochemical techniques, the analysis of released metal ions was performed by inductively coupled plasma mass spectrometry (ICP-MS) and the cytotoxicity was investigated in a culture of murine osteoblasts cells. Total immunity to localized corrosion in simulated body fluid (SBF) solutions was exhibited by Lotus-type porous Ni-free stainless steels, while Lotus-type porous AISI 316L showed very low pitting corrosion resistance evidenced by pitting corrosion at a very low breakdown potential. Additionally, Lotus-type porous Ni-free stainless steels showed a quite low metal ion release in SBF solutions. Furthermore, cell culture studies showed that the fabricated materials were non-cytotoxic to mouse osteoblasts cell line. On the basis of these results, it can be concluded that the investigated alloys are biocompatible and corrosion resistant and a promising material for biomedical applications.

  1. Students' Alternative Conceptions about the Lotus Effect: To Confront or to Ignore?

    Science.gov (United States)

    Kubisch, Franziska; Heyne, Thomas

    2016-01-01

    At the botanical garden of the University of Würzburg, we conducted practical lessons on bionics, focused on the lotus effect, with 260 students. Those approx. 14 years old, 8th-grade mid-level students were divided into two groups. During an instructional discussion about the topic, one group was confronted with their alternative conceptions…

  2. Sound Absorption and Friction Properties of Nano-Lotus Leaf Coated Concrete for Rigid Pavement

    Directory of Open Access Journals (Sweden)

    Marcelo GONZALEZ

    2016-09-01

    Full Text Available This paper presents the feasibility of superhydrophobic films to create the nano-lotus leaf effect on concrete surface and their influence on sound absorption and friction properties of concrete for application in rigid pavements. The study involved an evaluation of nanomaterials at the laboratory scale to analyze the effects of microtexture modification on the friction and sound absorption of concrete pavement. A number of laboratory specimens were produced by applying different amounts of nano-lotus leaf coating on the top of the textured concrete surface. The British pendulum test was used to measure the friction number, and an impedance tube was used to determine the sound absorption coefficient. Laboratory results indicate that nano-lotus leaf coated concrete can maintain the required friction property for rigid pavement, but may not increase the noise absorption. Further research must be carried out to determine possible benefit of the lotus leaf effect for reducing hydroplaning, particularly during heavy rainfall.DOI: http://dx.doi.org/10.5755/j01.ms.22.3.7638

  3. In vivo trypanocidal activity of Nymphaea lotus Linn. methanol extract against Trypanosoma brucei brucei

    Directory of Open Access Journals (Sweden)

    Muhammad Haruna Garba

    2015-10-01

    Full Text Available Objective: To evaluate the antitrypanosomal potentials of methanol extract of Nymphaea lotus Linn. (N. lotus with the aim of obtaining a new lead for formulating safe, inexpensive, nontoxic and readily available trypanocidal drugs. Methods: Seventy percent (v/v (methanol/water crude extract of N. lotus was evaluated for antitrypanosomal activity in experimental trypanosomiasis using Trypanosoma brucei bruceiinfected mice. Infected mice in different groups were administered intraperitoneally 100, 200, 300 and 400 mg/kg body weight/day of the crude for two weeks, while a positive control group was treated with standard drug, berenil. Results: The crude extract at a dose of 100 mg/kg body weight/day was more effective than the higher doses in completely clearing parasites from the blood of mice infected with Trypanosoma brucei brucei. Pre-treatment of healthy mice with the crude extract for 5 days before infection did not prevent the establishment of the infection, indicating that the extract had no prophylactic activity. Subinoculation of the blood and cerebrospinal fluid drawn from the cured mice into healthy mice failed to produce any infection within 50 days post inoculation. Administration of 1 000 mg/kg body weight of the crude extract led to the death of 50% of the experimental animals indicating a high level of toxicity of the extract at higher doses. Conclusions: This study has demonstrated the potency of the crude extract of N. lotus in treating experimental trypanosomiasis at lower doses.

  4. Early Lotus japonicus root transcriptomic responses to symbiotic and pathogenic fungal exudates

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    Marco eGiovannetti

    2015-06-01

    Full Text Available The objective of this study is to evaluate Lotus japonicus transcriptomic responses to arbuscular mycorrhizal (AM germinated spore exudates (GSE, responsible for activating nuclear Ca2+ spiking in plant root epidermis. A microarray experiment was performed comparing gene expression in Lotus rootlets treated with GSE or water after 24 h and 48 h. The transcriptional pattern of selected genes that resulted to be regulated in the array was further evaluated upon different treatments and timings. In particular, Lotus rootlets were treated with: GSE from the pathogenic fungus Colletotrichum trifolii; short chitin oligomers (acknowledged AM fungal signals and long chitin oligomers (as activators of pathogenic responses. This experimental set up has revealed that AM GSE generates a strong transcriptomic response in Lotus roots with an extensive defense-related response after 24 hours and a subsequent downregulation after 48 hours. A similar subset of defense-related genes resulted to be upregulated also upon treatment with C. trifolii GSE, although with an opposite trend. Surprisingly, long chitin oligomers activated both defense-like and symbiosis-related genes. Among the genes regulated in the microarray, promoter-GUS assay showed that LjMATE1 activates in epidermal cells and root hairs.

  5. STRATEGI PEMASARAN PAKET INBOUND TOUR: STUDI KASUS DI PT. LOTUS ASIA TOURS JIMBARAN BALI

    Directory of Open Access Journals (Sweden)

    Camelia Agatha Mahayu Putri

    2015-12-01

    Full Text Available Tujuan penelitian ini adalah untuk mengetahui faktor internal yaitu kekuatan dan kelemahan dan eksternal yaitu peluang dan ancaman dari PT. Lotus Asia Tours serta untuk mengetahui strategi yang dapat dilakukan pihak PT. Lotus Asia Tours untuk memasarkan paket inbound tour. Lokasi penelitian dilakukan di PT. Lotus Asia Tours yang terletak di Jl. By Pass Ngurah Rai No. 18, Jimbaran Bali.. Penelitian ini menggunakan metode deskriptif kualitatif. Teknik pengumpulan data dilakukan dengan cara observasi, wawancara, studi kepustakaan, studi dokumentasi dan didukung dengan daftar pertanyaan yang disebarkan kepada 60 informan. Daftar pertanyaan yang disebarkan berpedoman pada indikator marketing mix 7 P (product, price, place, promotion, physical evidence, people, process. Hasil data yang diperoleh lalu dianalisis dengan IFAS (Internal Factor Analysis Summary dan EFAS ( External Factor Analysis Summary, selanjutnya untuk mengetahui posisi perusahaan digunakan matriks internal eksternal dan untuk mengetahui alternatif strategi digunakan matriks SWOT, sehingga dapat menciptakan strategi pemasaran baru serta mengembangkan program-program yang telah ada agar dapat diterapkan oleh PT. Lotus Asia Tours Jimbaran Bali. Berdasarkan hasil penelitian diperoleh total nilai rata-rata lingkungan internal yaitu sebesar 3,01 dan lingkungan eksternal yaitu sebesar 2,96, dapat diketahui bahwa posisi perusahaan berada di sel IV yang berarti stability strategy dimana strategi yang tetapkan tidak merubah arah strategi yang telah ada.

  6. 78 FR 15114 - Group Lotus plc; Grant of Petition for a Temporary Exemption From an Advanced Air Bag Requirement...

    Science.gov (United States)

    2013-03-08

    ...This notice grants the petition of Group Lotus plc (Lotus) for a temporary exemption of the front passenger position of its Evora model from one advanced air bag requirement of FMVSS No. 208, i.e., the higher maximum speed (56 km/h (35 mph)) belted test requirement using 5th percentile adult female dummies. The agency finds that achieving compliance with that requirement would cause substantial economic hardship to Lotus and that the company has tried to comply with the requirement in good faith.

  7. Sacred messages for AIDS prevention. Principles into practice.

    Science.gov (United States)

    Galvao, J

    1991-01-01

    /religious leader well known tales were adapted to relay more information on health and AIDs in particular. 3 stories were presented in comic strip form and designed by a professional. The manual was titled ODO YA] and finished in August 1991. 50,000 copies will be distributed to religious leaders and 20,000 pamphlets to followers. Success is uncertain, but the project is exciting and hopeful in utilizing a rich symbolic tradition and informal groups far removed from the formal power structure and sacred traditions.

  8. Dissecting the 'bacon and eggs' phenotype: transcriptomics of post-anthesis colour change in Lotus.

    Science.gov (United States)

    Boehm, Mannfred M A; Ojeda, Dario I; Cronk, Quentin C B

    2017-10-17

    Post-anthesis colour change (PACC) is widely thought to be an adaptation to signal floral suitability to pollinators. Lotus filicaulis and Lotus sessilifolius are insect-pollinated herbaceous legumes with flowers that open yellow, shift to orange and finally red. This study examines the molecular basis for floral colour change in these Lotus species. Lotus filicaulis was cultivated in a glasshouse from which pollinating insects (bees) were excluded, and the rate of colour change was recorded in both unpollinated and manually pollinated flowers. Unpollinated flowers from both the yellow stage and the red stage were sampled for sequencing. The transcriptomes of L. filicaulis and L. sessilifolius of both colour stages were analysed for differentially expressed genes and enriched ontologies. The rate of progression through PACC doubled when L. filicaulis was hand-pollinated. De novo assembly of RNA-Seq reads from non-model Lotus species outperformed heterologous alignment of reads to the L. japonicus genome. Differential expression analysis suggested that the carotenoid biosynthetic pathway is upregulated at anthesis while the flavonoid biosynthetic pathway is upregulated with the onset of PACC in L. filicaulis and L. sessilifolius . Pollination significantly accelerates PACC in L. filicaulis , consistent with the hypothesis that PACC increases pollination efficiency by directing pollinators to unpollinated flowers. RNA-Seq results show the synchronized upregulation of the entire cyanidin biosynthesis pathway in the red stage of PACC in L. filicaulis and L. sessilifolius . The genes implicated offer the basis for further investigations into how gene families, transcription factors and related pathways are likely to be involved in PACC. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  9. Sacred Groves: Myths, Beliefs, and Biodiversity Conservation—A Case Study from Western Himalaya, India

    Directory of Open Access Journals (Sweden)

    Sushma Singh

    2017-01-01

    Full Text Available Religious and traditional beliefs, cultural mores, and practices play a crucial role in the conservation of environment and biodiversity. The present paper describes a case study of two sacred groves in Western Himalaya. Sacred groves (SGs are patches of land that are communally protected with religious zeal. A preliminary survey was conducted in these SGs to study their role in biodiversity conservation. The data collected included the general information regarding the SGs and the associated deity, nearest human habitation, access to them, and their floral and faunal diversity. Ethnomedicinal property of plants was collected from the indigenous communities. Many taboos are associated with both the SGs, which help in managing resources well through ritual representation. Different festivals are organized, where the local communities reaffirm their commitment to the forest and the deity. Sacred groves, in general, are a valuable tool of biodiversity conservation. But people’s changing attitudes, erosion of traditional beliefs, and human impact have caused degradation of sacred groves over the years. Their conservation would not be possible without the active participation of the local people. By improving their living standards and by giving benefits of conservation to them, long-term conservation goals in these SGs can be achieved.

  10. Friedrich Max Müller and the Sacred Books of the East

    NARCIS (Netherlands)

    Molendijk, Arie L.

    2016-01-01

    This volume offers a critical analysis of one the most ambitious editorial projects of late Victorian Britain: the edition of the fifty substantial volumes of the Sacred Books of the East (1879-1910). The series was edited and conceptualized by Friedrich Max Müller (1823-1900), a world-famous

  11. Book Review: Sonic spaces of the Karoo: The sacred music of a ...

    African Journals Online (AJOL)

    Marie Jorritsma. Sonic spaces of the Karoo: The sacred music of a South African coloured community. Temple University Press, 2011. ISBN 9781439902370, 201pp. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Article Metrics. Metrics Loading ... Metrics powered by ...

  12. "Approaching the Sacred": Directionality in the Relation between Curriculum and Knowledge Structure

    Science.gov (United States)

    Gamble, Jeanne

    2014-01-01

    Increasing pressure on all levels of educational provision, whether academic or overtly vocational, to be to "relevant" and "useful" prompts consideration of the relation between curriculum and pedagogy in terms of the internal structure of knowledge forms. Following Durkheim's distinction between "sacred" and…

  13. Place Attachment in the Bible : The role of attachment to sacred places in religious life

    NARCIS (Netherlands)

    Counted, Agina Victor; Watts, Fraser

    2017-01-01

    This paper examines the role of place attachment in religious life by analyzing various significant place events in the Bible, using analysis of biblical discourse. The paper looks at various biblical places, and explores the implications of approaching these sacred settings in terms of place

  14. Sacred and totemic plants among thirty two ethnic groups in Burkina ...

    African Journals Online (AJOL)

    This study aims to evaluate the diversity of sacred species and totemic species according to the different ethnic groups of Burkina Faso and to determine the influence of these taboos on the conservation of biodiversity. A sample of 2503 people from 32 ethnic groups were investigated in 330 villages based on the liveliness ...

  15. The Ontological Concept of Sacred and Profane in Islam (analytic study

    Directory of Open Access Journals (Sweden)

    Abdulnaser Sultan Mohsen

    2014-06-01

    Full Text Available The term ‘sacred’ in the study of religions appeared at the beginning of the last century; Western scholars studied primitive religions in the world and combined it with the term of ‘profane’ in an ambivalent or a deterministic relationship. This study is questing the concepts of sacred and profane through Islamic prospective of ontology, in which it confirms that the dualism of sacred and profane is involved in the spirit existence, not in the natural or material as the Western thought alleges. It is through Muslim scholars’ study of spirit and existence, and linguistic study of sacred and profane that has clarified that the existence has sacredness as much as granted by spirit. Much is also the achievement of perfect existence, or retrieval of pure spirit and not retrieval of the origin of creation according to Christian thought. Otherwise, the existence became profane when it has been disintegrated by obstruction of spirit functions, or losing of the spirit totally. Therefore, the concept of sacred in Islam is the perfect existence by spirit; conversely, the profane is isintegration of spirit.

  16. Separation of Oligosaccharides from Lotus Seeds via Medium-pressure Liquid Chromatography Coupled with ELSD and DAD

    Science.gov (United States)

    Lu, Xu; Zheng, Zhichang; Miao, Song; Li, Huang; Guo, Zebin; Zhang, Yi; Zheng, Yafeng; Zheng, Baodong; Xiao, Jianbo

    2017-03-01

    Lotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coupled with evaporative light-scattering detector and diode-array detector, five oligosaccharides were isolated and their structures were further characterized by electrospray ionization-mass spectrometry and gas chromatography-mass spectrometry. In vitro testing determined that LOS3-1 and LOS4 elicited relatively good proliferative effects on Lactobacillus delbrueckii subsp. bulgaricus. These results indicated a structure-function relationship between the physiological activity of oligosaccharides in lotus seeds and the number of probiotics applied, thus providing room for improvement of this particular feature. Intestinal probiotics may potentially become a new effective drug target for the regulation of immunity.

  17. The plant growth promoting substance, lumichrome, mimics starch and ethylene-associated symbiotic responses in lotus and tomato roots

    Directory of Open Access Journals (Sweden)

    Liezel eGouws

    2012-06-01

    Full Text Available Symbiosis involves responses that maintain the plant host and symbiotic partner’s genetic program; yet these cues are far from elucidated. Here we describe the effects of lumichrome, a flavin identified from Rhizobium spp., applied to lotus (Lotus japonicus and tomato (Solanum lycopersicum. Combined transcriptional and metabolite analyses suggest that both species shared common pathways that were altered in response to this application under replete, sterile conditions. These included genes involved in symbiosis, as well as transcriptional and metabolic responses related to enhanced starch accumulation and altered ethylene metabolism. Lumichrome priming also resulted in altered colonization with either Mesorhizobium loti (for lotus or Glomus intraradices/Glomus mossea (for tomato. It enhanced nodule number but not nodule formation in lotus; while leading to enhanced hyphae initiation and delayed arbuscule maturation in tomato.

  18. The Plant Growth Promoting Substance, Lumichrome, Mimics Starch, and Ethylene-Associated Symbiotic Responses in Lotus and Tomato Roots

    Science.gov (United States)

    Gouws, Liezel M.; Botes, Eileen; Wiese, Anna J.; Trenkamp, Sandra; Torres-Jerez, Ivone; Tang, Yuhong; Hills, Paul N.; Usadel, Björn; Lloyd, James R.; Fernie, Alisdair R.; Kossmann, Jens; van der Merwe, Margaretha J.

    2012-01-01

    Symbiosis involves responses that maintain the plant host and symbiotic partner’s genetic program; yet these cues are far from elucidated. Here we describe the effects of lumichrome, a flavin identified from Rhizobium spp., applied to lotus (Lotus japonicus) and tomato (Solanum lycopersicum). Combined transcriptional and metabolite analyses suggest that both species shared common pathways that were altered in response to this application under replete, sterile conditions. These included genes involved in symbiosis, as well as transcriptional and metabolic responses related to enhanced starch accumulation and altered ethylene metabolism. Lumichrome priming also resulted in altered colonization with either Mesorhizobium loti (for lotus) or Glomus intraradices/G. mossea (for tomato). It enhanced nodule number but not nodule formation in lotus; while leading to enhanced hyphae initiation and delayed arbuscule maturation in tomato. PMID:22701462

  19. Characterization of Strains unlike Mesorhizobium loti That Nodulate Lotus spp. in Saline Soils of Granada, Spain ▿ †

    Science.gov (United States)

    Lorite, María J.; Muñoz, Socorro; Olivares, José; Soto, María J.; Sanjuán, Juan

    2010-01-01

    Lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. The aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with Lotus spp. A total of 180 isolates able to nodulate Lotus corniculatus and Lotus tenuis from two locations in Granada, Spain, were characterized. Molecular identification of the isolates was performed by repetitive extragenic palindromic PCR (REP-PCR) and 16S rRNA, atpD, and recA gene sequence analyses, showing the presence of bacteria related to different species of the genus Mesorhizobium: Mesorhizobium tarimense/Mesorhizobium tianshanense, Mesorhizobium chacoense/Mesorhizobium albiziae, and the recently described species, Mesorhizobium alhagi. No Mesorhizobium loti-like bacteria were found, although most isolates carried nodC and nifH symbiotic genes closely related to those of M. loti, considered the type species of bacteria nodulating Lotus, and other Lotus rhizobia. A significant portion of the isolates showed both high salt tolerance and good symbiotic performance with L. corniculatus, and many behaved like salt-dependent bacteria, showing faster growth and better symbiotic performance when media were supplemented with Na or Ca salts. PMID:20435777

  20. Genome analysis methods: Lotus japonicus [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available Lotus japonicus Draft 2n=12 472 Mb 2008 Sanger (Clone-based) ... 315.1 Mb 3-5x Parace...l Genome Assembler 954 110,940 Kazusa Annotation PipelinE for Lotus japonicus (KAPSEL) 37,971 (v2.5) KDRI; http://www.kazusa.or.jp/lotus/ v2.5 v2.5 10.1093/dnares/dsn008 18511435 ...

  1. Antidiabetic Activity of a Lotus Leaf Selenium (Se)-Polysaccharide in Rats with Gestational Diabetes Mellitus.

    Science.gov (United States)

    Zeng, Zhaohui; Xu, Yun; Zhang, Bin

    2017-04-01

    A selenium (Se)-containing polysaccharide, lotus leaf selenium (Se)-polysaccharide (LLP), was isolated from a lotus leaf. The effects of LLP on antioxidant enzyme activities and insulin resistance in pregnant rats with gestational diabetes mellitus (GDM) were investigated. LLP administered orally at two doses (50 and 100 mg/kg) could significantly reverse the weight loss of pregnant rats before the delivery, fetal rats, and placentas in GDM rats (P fasting blood glucose (FBG) and fasting blood insulin (FINS) levels in GDM rats, but an increase of hepatic glycogen content, when compared with those in GDM rats (P  0.05). All the data indicated that LLP may be a promising drug candidate or a healthcare food for GDM therapy or protection.

  2. The Lotus japonicus ndx gene family is involved in nodule function and maintenance

    DEFF Research Database (Denmark)

    Grønlund, Mette; Gustafsen, Camilla; Jensen, Dorthe Bødker

    2003-01-01

    To elucidate the function of the ndx homeobox genes during the Rhizobium-legume symbiosis, two Lotus japonicus ndr genes were expressed in the antisense orientation under the control of the nodule-expressed promoter Psenod12 in transgenic Lotus japonicus plants. Many of the transformants obtained...... segregated into plants that failed to sustain proper development and maintenance of root nodules concomitant with down-regulation of the two ndx genes. The root nodules were actively fixing nitrogen 3 weeks after inoculation, but the plants exhibited a stunted growth phenotype. The nodules on such antisense...... supplied to the plants in which the two ndx genes are down-regulated. The results presented here, indicate that the ndx genes play a role in the development of structural nodule features, required for proper gas diffusion into the nodule and/or transport of the assimilated nitrogen to the plant....

  3. The evolution of plant chemical defence - new roles for hydroxynitrile glucosides in Lotus japonicus

    DEFF Research Database (Denmark)

    Knudsen, Camilla

    tissue disruption, their hydrolysis by a specific β-glucosidase results in the release of toxic hydrogen cyanide. Hydrolysis of the non-cyanogenic rhodiocyanosides by β-glucosidase activity is shown to produce an anti-fungal furanone. The biosynthetic pathways for these related hydroxynitrile glucosides...... share the first step, and paralogous enzymes with distinct roles, such as the UDP-glucosyltransferases UGT85K2 and UGT85K3, catalyse subsequent reactions. The results presented in this PhD thesis provide unique insight in the biosynthesis of hydroxynitrile glucosides in Lotus japonicus in terms of gene...... function and evolution. Further, it contributes to our understanding of the formation and role of biosynthetic gene clusters in plant chemical defence. The bifurcation in hydroxynitrile glucoside biosynthesis and catabolism observed in Lotus japonicus makes it a very suitable model system to study...

  4. 3D Printing of Lotus Root-Like Biomimetic Materials for Cell Delivery and Tissue Regeneration.

    Science.gov (United States)

    Feng, Chun; Zhang, Wenjie; Deng, Cuijun; Li, Guanglong; Chang, Jiang; Zhang, Zhiyuan; Jiang, Xinquan; Wu, Chengtie

    2017-12-01

    Biomimetic materials have drawn more and more attention in recent years. Regeneration of large bone defects is still a major clinical challenge. In addition, vascularization plays an important role in the process of large bone regeneration and microchannel structure can induce endothelial cells to form rudimentary vasculature. In recent years, 3D printing scaffolds are major materials for large bone defect repair. However, these traditional 3D scaffolds have low porosity and nonchannel structure, which impede angiogenesis and osteogenesis. In this study, inspired by the microstructure of natural plant lotus root, biomimetic materials with lotus root-like structures are successfully prepared via a modified 3D printing strategy. Compared with traditional 3D materials, these biomimetic materials can significantly improve in vitro cell attachment and proliferation as well as promote in vivo osteogenesis, indicating potential application for cell delivery and bone regeneration.

  5. Secondary Fitometabolitos that affect the nutritional value of Lotus corniculatus as forage for ruminant animals

    OpenAIRE

    Luz Elena Santacoloma Varón

    2014-01-01

    In the highlands of Bogota at 2600 masl, temperature 14°C and 1013 mm of precipitation, three plots have been furbished by modifying the physico- chemical conditions of the soil and planted the species Lotus corniculatus to evaluate content of condensed tannins, hydrolysable tannins, phenols, tannins that precipitate protein, saponins and alkaloids. The purpose was to determine the effect of soil conditions on the concentration of secondary phytobiomolecules. Analytical and instrumental techn...

  6. Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation.

    Science.gov (United States)

    Benammar, Chahid; Hichami, Aziz; Yessoufou, Akadiri; Simonin, Anne-Marie; Belarbi, Meriem; Allali, Hocine; Khan, Naim A

    2010-09-24

    Zizyphus lotus L. (Desf.) also known as Jujube, is a deciduous shrub which belongs to Rhamnaceae family. This plant is used in Algerian traditional medicine for its anti-diabetic, sedative, analgesic, anti-inflammatory and hypoglycaemic activities. In the present study, we determined the concentrations of different vitamins (vitamin A, C and E) and fatty acids in root, stem, leaves, fruit pulp and seed of Zizyphus lotus L. (Desf.) and assessed the effects of their aqueous extracts on antioxidant status and human T-cell proliferation. Aqueous filtrates from different parts, i.e, root, leaf, stem, fruit pulp and seed, of Zizyphus lotus L. (Desf.) were prepared. Vitamin C levels were determined by precipitating with 10% trichloroacetic acid and vitamin A and E were assessed by HPLC. Lipid composition of these extracts was determined by gas-liquid chromatography. Anti-oxidant capacity was evaluated by using anti-radical resistance kit [Kit Radicaux Libres (KRL@; Kirial International SA, Couternon, France)]. T-cell blastogenesis was assessed by the incorporation of 3H-thymidine. IL-2 gene expression was evaluated by RT-qPCR. Our results show that fruit pulp contained higher vitamin A and C contents than other parts of the plant. Furthermore, the fruit pulp was the richest source of linoleic acid (18:2n-6), a precursor of n-6 fatty acids. Fruit seeds possessed higher vitamin C levels than leaves, roots and stem. The leaves were the richest source of vitamin E and linolenic acid (18:3n-3), a precursor of n-3 fatty acids. The antioxidant capacity of the different extracts, measured by KRL@ test, was as follows: pulp Zizyphus lotus L. (Desf.) exerted immunosuppressive effects. Seed extracts exerted the most potent immunosuppressive effects on T cell proliferation and IL-2 mRNA expression. The results of the present study are discussed in the light of their use to modulate the immune-mediated diseases.

  7. Pterostichus neilgaimani sp. nov., a new species of ground beetles (Coleoptera: Carabidae) from relict sacred grove in Eastern Georgia.

    Science.gov (United States)

    Chaladze, Giorgi; Kalatozishvili, Levan; Janiashvili, Zurab; Bakuradze, Giorgi

    2017-10-03

    A new species of ground beetles (Coleoptea: Carabidae) belonging to the subgenus Aphaonus Reitter, 1887 (genus Pterostichus Bonelli, 1810) is described, based on two specimens collected from the sacred grove of Khevsha (Eastern Georgia).

  8. A new method for producing "Lotus Effect" on a biomimetic shark skin.

    Science.gov (United States)

    Liu, Yunhong; Li, Guangji

    2012-12-15

    Nature has long been an important source of inspiration for mankind to develop artificial ways to mimic the remarkable properties of biological systems. In this work, a new method was explored to fabricate a superhydrophobic dual-biomimetic surface comprising both the shark-skin surface morphology and the lotus leaf-like hierarchical micro/nano-structures. The biomimetic surface possessing shark-skin pattern microstructure was first fabricated by microreplication of shark-skin surface based on PDMS; and then it was treated by flame to form hierarchical micro/nano-structures that can produce lotus effect. The fabricated biomimetic surfaces were characterized with scanning electron microscopy (SEM), water contact angle measurements and liquid drop impact experiments. The results show that the fabricated dual-biomimetic surface possesses both the vivid shark-skin surface morphology and the lotus leaf-like hierarchical micro/nano-structures. It can exhibit excellent superhydrophobicity that the contact angle is as high as 160° and maintain its robustness of the superhydrophobicity during the droplet impact process at a relatively high Weber number. The mechanism of the micromorphology evolution and microstructural changes on the biomimetic shark-skin surface was also discussed here in the process of flame treatment. This method is expected to be developed into a novel and feasible biomimetic surface manufacturing technique. Copyright © 2012 Elsevier Inc. All rights reserved.

  9. Self-propulsion of dew drops on lotus leaves: a potential mechanism for self cleaning.

    Science.gov (United States)

    Watson, Gregory S; Gellender, Marty; Watson, Jolanta A

    2014-01-01

    This study shows that condensation on the hierarchically structured lotus leaf can facilitate self-propulsion of water droplets off the surface. Droplets on leaves inclined at high angles can be completely removed from the surface by self-propulsion with the assistance of gravity. Due to the small size of mobile droplets, light breezes may also fully remove the propelled droplets, which are typically projected beyond the boundary layer of the leaf cuticle. Moreover the self-propelled droplets/condensate were able to remove contaminants (eg silica particles) from the leaf surface. The biological significance of this process may be associated with maintaining a healthy cuticle surface when the action of rain to clean the surface via the lotus effect is not possible (due to no precipitation). Indeed, the native lotus plants in this study were located in a region with extended time periods (several months) without rain. Thus, dew formation on the leaf may provide an alternative self-cleaning mechanism during times of drought and optimise the functional efficiency of the leaf surface as well as protecting the surface from long term exposure to pathogens such as bacteria and fungi.

  10. A biomimetic nano hybrid coating based on the lotus effect and its anti-biofouling behaviors

    Science.gov (United States)

    Li, Jiang; Wang, Guoqing; Meng, Qinghua; Ding, Chunhua; Jiang, Hong; Fang, Yongzeng

    2014-10-01

    To develop an environmentally friendly anti-biofouling coating in virtue of bionics, a block copolymer containing fluorine (Coplm_F) of low surface energy was prepared by copolymerization. The Ag-loaded mesoporous silica (Ag@SBA) acting as a controlled-release antifoulant was prepared from the mesoporous silica (SBA-15). The nano hybrid coating (Ag@SBA/Coplm_F) composing of the Coplm_F and Ag@SBA was to biomimetically simulate the lotus microstructure. The concentration of fluorine element on surface was analyzed by the energy dispersive spectroscopy (EDS) and found rising to 1.45% after hybridation, which could be explained by the driving effect of SBA-15 via the hydrogen bond. This nanoscale morphology of the hybrid coating was measured and found highly semblable to the microstructure of the lotus surface. The contact angle was determined as 151° which confirmed the superhydrophobicity and lotus effect. The adhesion behaviors of Pseudomonas fluorescens, Diatoms, and Chlorella on the surface of the nano hybrid coating (Ag@SBA/Coplm_F) were studied and good effects of anti-biofouling were observed.

  11. Tunable hydrodynamic characteristics in microchannels with biomimetic superhydrophobic (lotus leaf replica) walls.

    Science.gov (United States)

    Dey, Ranabir; Raj M, Kiran; Bhandaru, Nandini; Mukherjee, Rabibrata; Chakraborty, Suman

    2014-05-21

    The present work comprehensively addresses the hydrodynamic characteristics through microchannels with lotus leaf replica (exhibiting low adhesion and superhydrophobic properties) walls. The lotus leaf replica is fabricated following an efficient, two-step, soft-molding process and is then integrated with rectangular microchannels. The inherent biomimetic, superhydrophobic surface-liquid interfacial hydrodynamics, and the consequential bulk flow characteristics, are critically analyzed by the micro-particle image velocimetry technique. It is observed that the lotus leaf replica mediated microscale hydrodynamics comprise of two distinct flow regimes even within the low Reynolds number paradigm, unlike the commonly perceived solely apparent slip-stick dominated flows over superhydrophobic surfaces. While the first flow regime is characterized by an apparent slip-stick flow culminating in an enhanced bulk throughput rate, the second flow regime exhibits a complete breakdown of the aforementioned laminar and uni-axial flow model, leading to a predominantly no-slip flow. Interestingly, the critical flow condition dictating the transition between the two hydrodynamic regimes is intrinsically dependent on the micro-confinement effect. In this regard, an energetically consistent theoretical model is also proposed to predict the alterations in the critical flow condition with varying microchannel configurations, by addressing the underlying biomimetic surface-liquid interfacial conditions. Hence, the present research endeavour provides a new design-guiding paradigm for developing multi-functional microfluidic devices involving biomimetic, superhydrophobic surfaces, by judicious exploitation of the tunable hydrodynamic characteristics in the two regimes.

  12. Phytosociological analysis of a traditionally managed sacred grove in transitional ecosystem of eastern lateritic part of India

    OpenAIRE

    Sumit Manna; Tushar Kanti Ghara; Debal Ray; Anirban Roy

    2013-01-01

    Background: Traditionally managed sacred groves in the lateritic parts of eastern India are a common phenomenon. Groves located in the transitional ecosystem are characteristically rich in biodiversity and are found to be showcasing local refuges. Besides the cultural and aesthetic needs of mankind, these ancient forest remnants are the center of attraction to the researchers for their immense veritable gene pool thriving on sacred belief. Present study highlights the phytosociological charac...

  13. Impact and ecosystem service of forest and sacred grove as saviour of water quantity and quality in Garhwal Himalaya, India.

    Science.gov (United States)

    Jana, Purna; Dasgupta, Sabyasachi; Todaria, Nagendra P

    2017-08-29

    The present study was conducted in environs of the sacred grove of Garhwal Himalaya, India, with a view to assess the impacts of sacred groves and forests on the quality and quantity of water and also to assess the effect of seasonality on perennial stream quality. Water samples were collected from three randomly selected stream spots of both the sacred grove dominated by deodar (Cedrus deodara) and the non-sacred patch dominated by oak (Quercus leucotrichophora). Water samples from both patches were within the World Health Organization (WHO) standard limits. Based on an already established water quality index, water quality of both patches was safe for domestic and irrigation purposes but needs treatment for drinking purposes. Results of the present study also showed a very prominent impact of forest type as well as management condition on water quality and quantity. The water discharge from an oak forest shows more consistency than the discharge from a deodar forest. Due to the presence of the sacred grove, the area has become the source of good quality water supply during lean season for the surrounding villages. Water quality and quantity differed along with the change in season. The sacred grove and the existing forest leave a great impression on local dwellers, as due to its presence, local dwellers never run out of water supply during the dry season. As a result, the villagers sincerely want to protect the area for the sake of their own well-being.

  14. The effect of methanol rhizome extract of Nymphaea lotus Linn. (Nymphaeaceae) in animal models of diarrhoea.

    Science.gov (United States)

    Bello, Fatima Hauwa; Maiha, Bilkisu B; Anuka, Joseph A

    2016-08-22

    Nymphaea lotus, which is widely distributed throughout tropical Africa, enjoys a number of ethnomedical uses in Nigeria. Traditionally, the rhizomes of N. lotus are used to cure diarrhoea. This study aims to evaluate the antidiarrhoeal activity of the methanol rhizome extract of N. lotus plant in laboratory animals. The extract was screened for activity against castor oil-induced diarrhoea and magnesium sulphate-induced diarrhoea as well as effect on gastric transit time in mice. The effect of methanol rhizome extract of Nymphaea lotus on the perfused isolated tissue preparation was also determined. For castor oil-induced diarrhoea, the extract at doses of 200, 400 and 800mg/kg produced significant reduction in the frequency of diarrhoea (at p<0.001, p<0.001 and p<0.01 respectively). The extract at 800mg/kg produced a significant delay in onset of diarrhoea (p<0.05) comparable to loperamide (3mg/kg). The frequency of magnesium sulphate-induced diarrhoea was also significantly reduced in the groups treated with 200, 400 and 800mg/kg of the extract at p<0.001, p<0.001 and p<0.01 respectively. At doses of 200mg/kg and 400mg/kg, the protection produced was comparable to loperamide, 3mg/kg. All treated groups produced significant reduction in the transit of charcoal meal along the intestinal tract at p<0.001. The extract at low concentration (4×10(-4)-6.4×10(-2)mg/ml) had contractile effect on the tone of contraction of the rabbit jejunum while at higher concentrations (8×10(-2)-512×10(-2)mg/ml) produced significant reduction in the tone and rate of spontaneous contraction of rabbit jejunum. The extract at lower concentrations (4×10(-4)-2×10(-2)mg/ml) has no effect on contraction of the guinea pig ileum while higher concentrations (4×10(-2)-512×10(-2)mg/ml) produced significant relaxant activity on guinea pig ileum. This study has shown that the methanol rhizome extract of N. lotus has antidiarrhoeal properties thus justifying its use by the local population for

  15. Sacred Torrents in Modernity: German Jewish Philosophers and the Legacy of Secularization

    Directory of Open Access Journals (Sweden)

    Roemer Nils

    2016-12-01

    Full Text Available This article investigates the ongoing interaction between the Jewish sacred past and its modern interpreters. Jewish thinkers from the eighteenth century reclaimed these ideals instead of dismissing them. Sacred traditions and modern secular thought existed in their mutual constitutive interdependence and not in opposition. When the optimism in historical progress and faith in reason unraveled in the fin de siècle, it engendered a new critical response by Jewish historians and philosophers of the twentieth century. These critical voices emerged within the fault lines of nineteenth and early twentieth century Jewish anti-historicist responses. What separated twentieth-century Jewish thinkers such as Martin Buber, Franz Rosenzweig, and Gershom Scholem from their nineteenth-century forerunners was not their embrace of religion but their critical stance toward reason and their crumbling faith in historical progress.

  16. Sacred scarab” in jewelry of Ancient Egypt: the symbol interpretation problem

    Directory of Open Access Journals (Sweden)

    Lada V. Prokopovich

    2015-03-01

    Full Text Available The image of the Sacred scarab, like any other fact of culture, should be considered only in the context of certain circumstances, which involve the approach choice: viewing as either a sign or a symbol. In those cases where the scarab image stands as a symbol, while interpreting this one it should be taken into account the cultural context, which, as relevant studies show, can never be reduced to a simple interpretation scheme “Scarab — Dorbeetle — Sun symbol”. In that connection suggested is a hypothesis, according to which the image of the Sacred scarab as a symbol is divided into two, at least, concepts: a “Sunny Beetle”, to mean the beetle species Cetonia aurata (or any other similar, and a “Beetle-Demiurge”, to mean the dorbeetle.

  17. Sacred hills of the Toda people of South India: A plea for world heritage status

    Science.gov (United States)

    Tarun. Chhabra

    2015-01-01

    Abstract-The Todas worship scores of hilltops where they believe their principal deities or clan-specific local gods reside. It is thus considered sacrilege even to point towards such a deity peak with one's finger. It is also no coincidence at all that the area in and around the Toda sacred-landscape, where their major hill deities are believed to reside, has...

  18. Does the sacred make a difference? Category formation in comparative religion

    Directory of Open Access Journals (Sweden)

    Veikko Anttonen

    1999-01-01

    Full Text Available When taken at face value, the sacred seems to be an unproblematic concept. Times, places, persons, animals and objects are classified as `sacred', because they have or have had a religious or spiritual significance for people in specific historical and social contexts. Religious traditions and their systems of signification are taken to explain why people have set aside specific things and considered them qualitatively different from other things. Deeming something as sacred means that it is disconnected from the category in social life in which similar things are classified and bestowed with special meaning and value. Sacredness of an object means that it stands in direct relationship to specific power-laden super-human entity by which members in a given culture mirror their self-consciousness or some aspects of it. A sanctuary for instance is a place that is set apart from the rest of the social space, because it is valued as a point of contact between man and the super-human agent worshipped by the local community.

  19. The Museumification of Rumi’s Tomb: Deconstructing Sacred Space at the Mevlana Museum

    Directory of Open Access Journals (Sweden)

    Rose Aslan

    2014-12-01

    Full Text Available Tourists and pilgrims from across Turkey and around the world flock to the tomb of Jalal al-Din Rumi (d. 1273, one of the greatest poets and Sufi masters in Islam. Since 1925, the Turkish government has relentlessly struggled to control Islamic influences in society and to channel people’s devotion to the memory of Kemal Ataturk (d. 1938 and his secular ideology. This article argues that by restructuring the layout and presentation of the tomb complex of Rumi, and putting the sacred space through the process of museumification, the Turkish state has attempted to regulate the place in order to control people’s experience of the sacred. The Museum functions simultaneously as a sacred place and a tourist site and the role of visitors as pilgrims and tourists is ambiguous. This article examines the history and politics of the space in order to illustrate how it functions as a site of contestation and how visitors act as important agents in the construction of the space’s meaning.

  20. Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-epsilon-caprolactone) as a blood compatible material.

    Science.gov (United States)

    Lim, Jin Ik; Kim, Seung il; Kim, Soo Hyun

    2013-03-01

    A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-ɛ-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14 μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering. Copyright © 2012 Elsevier B.V. All rights reserved.

  1. Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes

    Directory of Open Access Journals (Sweden)

    Chengjun Ma

    2014-02-01

    Full Text Available Aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-HIV’s activities. In order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (HSCCC was developed. Without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by HSCCC in one step. The separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v. In each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity, 1.1 mg of pronuciferine (96.8% purity, 8.5 mg of nuciferine (98.9% purity, and 2.7 mg of roemerine (97.4% respectively. The chemical structure of four aporphine alkaloids are identified by means of electrospray ionization MS (ESI-MS and nuclear magnetic resonance (NMR analysis. Moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3T3-L1 adipocytes. The results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did.

  2. Protective effect of Nelumbo nucifera extracts on beta amyloid protein induced apoptosis in PC12 cells, in vitro model of Alzheimer's disease

    Directory of Open Access Journals (Sweden)

    Alaganandam Kumaran

    2018-01-01

    Full Text Available Alzheimer's disease (AD is the most common cause of dementia in the elderly. β-Amyloid (Aβ has been proposed to play a role in the pathogenesis of AD. Deposits of insoluble Aβ are found in the brains of patients with AD and are one of the pathological hallmarks of the disease, but the underlying signaling pathways are poorly understood. In order to develop antidementia agents with potential therapeutic value, we examined the inhibitory effect of the Nelumbo nucifera seed embryo extracts on to the aggregated amyloid β peptide (agg Aβ1–40-induced damage of differentiated PC-12 cells (dPC-12, a well-known cell model for AD. In the present study, seed embryos of N. nucifera were extracted with 70% methanol in water and then separated into hexane, ethyl acetate, n-butanol, and water layers. Among them, only the n-butanol layer showed strong activity and was therefore subjected to separation on Sephadex LH-20 chromatography. Two fractions showing potent activity were found to significantly inhibit Aβ1–40 toxicity on dPC-12 cells in increasing order of concentration (10–50 μg/mL. Further purification and characterization of these active fractions identified them to be flavonoids such as rutin, orientin, isoorientin, isoquercetrin, and hyperoside. 2,2-Diphenyl-1-picrylhydrazyl hydrate scavenging activity of the extracts was also carried out to ascertain the possible mechanism of the activity.

  3. Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes

    Science.gov (United States)

    Ma, Chengjun; Wang, Jinjun; Chu, Hongmei; Zhang, Xiaoxiao; Wang, Zhenhua; Wang, Honglun; Li, Gang

    2014-01-01

    Aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-HIV’s activities. In order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (HSCCC) was developed. Without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by HSCCC in one step. The separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). In each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. The chemical structure of four aporphine alkaloids are identified by means of electrospray ionization MS (ESI-MS) and nuclear magnetic resonance (NMR) analysis. Moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3T3-L1 adipocytes. The results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did. PMID:24577311

  4. Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

    DEFF Research Database (Denmark)

    Dam, Svend Secher; Thaysen-Andersen, Morten; Stenkjær, Eva

    2013-01-01

    (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has...

  5. Contribution to Research of Mathematical Properties of Pre-Christian Slavic Sacred Landscape Structures

    Directory of Open Access Journals (Sweden)

    Andjelko Djermek

    2013-01-01

    Full Text Available Considerable amount of interpreted data indicates that the ancient Slavs positioned their sacred sites in a way which refers to characteristic Sun angles. The article addresses the question whether distances among such sites are based on a common unit of length. In particular, this article tackles that question applying the mathematical formalism on the following two assumptions: (i the absolute value of a distance between sacred sites was significant to the pre-Christian Slavic priests, along with the angles between lines connecting pairs of sites; (ii the distances were prevalently measured utilising the projections of isosceles right triangle on the horizontal plane, with the exceptions of flat grounds for which the distances were measured by walk. That assumption follows from the frequent occurrence of ratio 1:√2 in the analysed sacred sites. Based on the two stated assumptions the attempts are done to find the best possible length modules by using the probability distribution method of arithmetic sequences. The main property of length modules which are the least probable to appear by mere chance is that they account for as many as possible of distances from the analysed set of distances. The stated method is applied on numerous sacred systems described in literature. The result is that several common modules are extracted. The modules are subsequently correlated with the modules extracted in my recent article using the novel method which extracts the optimal common sub-module. Value of the length module thereby obtained is 30,9 m. It has 60 sub-units 0,515 m long (a cubit and 100 sub-units 0,309 m long (a foot. Multiples of 100 or 365 sub-units, respectively, are regularly encountered in the analysed set of sacred sites in the form of sub-harmonics of the observed distances. One may argue that results of the analysis of the distances contributed to the fact that the ancient Slavs were giving a lot of attention to a solar calendar and

  6. Expanding the Lotus japonicus reverse genetics toolbox – Development of LORE1 retrotransposon mutagenesis and artificial miRNA-mediated silencing

    DEFF Research Database (Denmark)

    Urbanski, Dorian Fabian

    2011-01-01

    . Prior to this project, the only reverse genetics resource available in Lotus was the TILLING resource. In an attempt to advance Lotus genetic studies, present study is focused on the development of two additional resources. The first is based on insertional mutagenesis and the second on harnessing post-transcriptional......Currently, the most common approach to studying Lotus japonicus (Lotus) genes is forward genetics in which a gene responsible for the studied phenotype is identified through map-based cloning. In reverse genetics, the activity of a gene of interest is modified to discover its mutant phenotype....... The protocols developed in the current project are now the cornerstone of a new LORE1 reverse genetics resource characterized by efficient mutant line generation and accurate mutation annotation. In parallel, artificial microRNAs (amiRNAs) were designed based on both Arabidopsis and Lotus backbones...

  7. Comet 67P observations with LOTUS: a new near-UV spectrograph for the Liverpool Telescope

    Science.gov (United States)

    Marchant, Jon; Jermak, Helen; Steele, Iain; Snodgrass, Colin; Fitzsimmons, Alan; Jones, Geraint

    2015-11-01

    The European Space Agency’s Rosetta spacecraft has been orbiting comet 67P/Churyumov-Gerasimenko (hereinafter “67P”) since August 2014, providing in-situ measurements of the dust, gas and plasma content of the coma within ~100km of the nucleus. Supporting the mission is a world-wide coordinated campaign of simultaneous ground-based observations of 67P (www.rosetta-campaign.net), providing wider context of the outer coma and tail invisible to Rosetta. We can now compare these observations, augmented by "ground truth" from Rosetta, with those of other comets past and future that are only observed from Earth.The robotic Liverpool Telescope (LT) is part of this campaign due to its unique ability to flexibly and autonomously schedule regular observations over entire semesters. Its optical imagery has recently been supplemented by near-UV spectroscopy to observe the UV molecular bands below 4000Å that are of considerable interest to cometary science. The LT's existing spectrographs FRODOSpec and SPRAT cut off at 4000Å, so the Liverpool Telescope Optical-to-UV Spectrograph - LOTUS - was fast-track designed, built and deployed on-sky in just five months. LOTUS contains no moving parts; acquisition is made with the LT's IO:O imaging camera, and different width slits for calibration and science are selected by fine-tuning the telescope's pointing on an innovative "step" design in its single slit.We present here details of the LOTUS spectrograph, and some preliminary results of our ongoing observations of comet 67P.

  8. Zizyphus lotus L. (Desf. modulates antioxidant activity and human T-cell proliferation

    Directory of Open Access Journals (Sweden)

    Belarbi Meriem

    2010-09-01

    Full Text Available Abstract Background Zizyphus lotus L. (Desf. also known as Jujube, is a deciduous shrub which belongs to Rhamnaceae family. This plant is used in Algerian traditional medicine for its anti-diabetic, sedative, analgesic, anti-inflammatory and hypoglycaemic activities. In the present study, we determined the concentrations of different vitamins (vitamin A, C and E and fatty acids in root, stem, leaves, fruit pulp and seed of Zizyphus lotus L. (Desf. and assessed the effects of their aqueous extracts on antioxidant status and human T-cell proliferation. Methods Aqueous filtrates from different parts, i.e, root, leaf, stem, fruit pulp and seed, of Zizyphus lotus L. (Desf. were prepared. Vitamin C levels were determined by precipitating with 10% trichloroacetic acid and vitamin A and E were assessed by HPLC. Lipid composition of these extracts was determined by gas-liquid chromatography. Anti-oxidant capacity was evaluated by using anti-radical resistance kit [Kit Radicaux Libres (KRL@; Kirial International SA, Couternon, France]. T-cell blastogenesis was assessed by the incorporation of 3H-thymidine. IL-2 gene expression was evaluated by RT-qPCR. Results Our results show that fruit pulp contained higher vitamin A and C contents than other parts of the plant. Furthermore, the fruit pulp was the richest source of linoleic acid (18:2n-6, a precursor of n-6 fatty acids. Fruit seeds possessed higher vitamin C levels than leaves, roots and stem. The leaves were the richest source of vitamin E and linolenic acid (18:3n-3, a precursor of n-3 fatty acids. The antioxidant capacity of the different extracts, measured by KRL@ test, was as follows: pulp Zizyphus lotus L. (Desf. exerted immunosuppressive effects. Conclusion Seed extracts exerted the most potent immunosuppressive effects on T cell proliferation and IL-2 mRNA expression. The results of the present study are discussed in the light of their use to modulate the immune-mediated diseases.

  9. Chemical Characterization and Prebiotic Effect of Water-Soluble Polysaccharides from Zizyphus lotus Leaves

    OpenAIRE

    Zakaria Boual; Abdellah Kemassi; Toufik Chouana; Philippe Michaud; Mohammed Didi Ould El Hadj

    2015-01-01

    In order to investigate the prebiotic potential of oligosaccharides prepared by chemical hydrolysis of water-soluble polysaccharides (WSP) from Zizyphus lotus leaves, the effect of oligosaccharides on bacterial growth was studied. The chemical composition of WSP was evaluated by colorimetric assays revealed the average values: 7.05±0.73% proteins and 86.21±0.74% carbohydrates, among them 64.81±0.42% is neutral sugar and the rest 16.25±1.62% is uronic acids. The characteri...

  10. [Evaluation of antifungal and mollusuicidial activities of Moroccan Zizyphus lotus (L.) Desf].

    Science.gov (United States)

    Lahlou, M; El Mahi, M; Hamamouchi, J

    2002-11-01

    Zizyphus lotus (L.) Desf. is one of the traditional drugs commonly used in folk medicine in Morocco. Extracts obtained from the successive exhaustion in petroleum ether, chloroform, ethyl acetate and methanol were in vitro found active either against nine pathogenic fungi and Bulinus truncatus, the intermediate host and vector of transmission of unitary schistosomiasis in Morocco. Particularly, the chloroform extract appears the most interesting in antifungal tests at lowest concentrations because of its countenance on terpenic compounds. Whereas, methanolic extract was found to possess the strong mollusuicidial activity and exhibited potent "knock-down" effect on molluscans related to its countenance on saponins.

  11. The evolution of plant chemical defence - new roles for hydroxynitrile glucosides in Lotus japonicus

    DEFF Research Database (Denmark)

    Knudsen, Camilla

    Plants are sessile organisms well-known to produce a vast array of chemical compounds of which many are used in chemical defence against herbivores and pathogens. The biosynthesis of these plant chemical defence compounds poses a considerable risk of self-toxicity for the plant itself. Several...... function and evolution. Further, it contributes to our understanding of the formation and role of biosynthetic gene clusters in plant chemical defence. The bifurcation in hydroxynitrile glucoside biosynthesis and catabolism observed in Lotus japonicus makes it a very suitable model system to study...

  12. Sacred Choices: Adolescent Relationships and Sexual Ethics--The Reform Movement's Response to the Need for Faith-Based Sexuality Education

    Science.gov (United States)

    Winer, Rabbi Laura Novak

    2011-01-01

    "Sacred Choices: Adolescent Relationships and Sexual Ethics" is a sexual ethics curriculum for middle school and high school students developed by the Union for Reform Judaism. Sacred Choices strives to teach Reform Jewish teens that their bodies are gifts from God and that Judaism provides relevant guidance on how to use and care for that gift…

  13. Tuskegee as Sacred Rhetoric: Focal Point for the Emergent Field of African American Religion and Health.

    Science.gov (United States)

    Laws, Terri

    2018-02-01

    Scholars in African American religion engage the Tuskegee Syphilis Study as the focal point of the African American experience in institutional medicine. Seeking a way forward from this history and its intentional evil, the author proposes to position Tuskegee as a form of Lynch's culturally contextual sacred rhetoric to make use of its metaphoric value in the emerging field of African American religion and health. In this broader meaning-making frame, Tuskegee serves as a reminder that African American religious sensibility has long been an agential resource that counters abuse of the Black body. It also acknowledges the complex decisions facing African American clinical trial participants.

  14. Tracce d’inizio e di fine. Citazioni sacre nelle “17 variazioni” di Emilio Villa

    Directory of Open Access Journals (Sweden)

    Bianca Battilocchi

    2015-12-01

    Full Text Available This article aims to identify the numerous quotations from sacred texts appearing in Emilio Villa’s 17 variazioni. Indeed, the 1955 collection of poems offers a wide range of references to Biblical sources and archaic cosmogonic myths, ancient symbologies and eschatological conjectures. The poet is both the translator and the interpreter of these texts, which the collection mixes and interlinks in the shape of an enigma. This air of mystery seems to deny any definitive revelation, highlighting instead a plurality of points of view that ultimately result in Apocalypse.

  15. A sacred dance transmitted in the ritual for paying homage to one's teacher in Thailand

    OpenAIRE

    岩澤, 孝子

    2001-01-01

    This article attemps to describe a dance which is considered as a sacred knowledge among classical dancers in Thailand. This is the reflection of their unique idea, wai khru, which means showing respect to the teacher and the ritual based on this idea. In Thailand wai khru is generally practised wherever they have a person to be recognized as a teacher in their own comunities. In these days, however, under the influence of the modernization, the idea that the teacher has a special power and k...

  16. Swords, Settlement and Sacred Places: The Archaeology of Brisley Farm, Ashford, Kent

    Directory of Open Access Journals (Sweden)

    Jim Stevenson

    2012-12-01

    Full Text Available Archaeology South-East (UCL has recently completed the post-excavation analysis of the results of the long-term Brisley Farm project, a series of large-scale archaeological investigations carried out in Kent since 1998. This research has revealed significant insights into the development of an ancient landscape located to the south of Ashford, spanning some three millennia.  Amongst the most revealing discoveries, which included two ‘warrior burials’, is evidence of how funerary and sacred concerns played a pivotal part in the Late Iron Age and Roman everyday life of the area.

  17. The role of multiscale roughness in the Lotus effect: is it essential for super-hydrophobicity?

    Science.gov (United States)

    Bittoun, Eyal; Marmur, Abraham

    2012-10-02

    The role of multiscale (hierarchical) roughness in optimizing the structure of nonwettable (superhydrophobic) solid surfaces was theoretically studied for 2D systems of a drop on three different types of surface topographies with up to four roughness scales. The surface models considered here were sinusoidal, flat-top pillars, and triadic Koch curves. Three criteria were used to compare between the various topographies and roughness scales. The first is the transition contact angle (CA) between the Wenzel (W) and Cassie-Baxter (CB) wetting states, above which the CB state is the thermodynamically stable one. The second is the solid-liquid (wetted) interfacial area, as an indicator for the ease of roll-off of a drop from the superhydrophobic surfaces. The third is the protrusion height that reflects the mechanical stability of the surface against breakage. The results indicate that multiscale roughness per se is not essential for superhydrophobicity; however, it mainly decreases the necessary protrusion height. Thus, multiscale roughness is beneficial for the Lotus effect mostly with regard to mechanical stability. The sinusoidal topography with three levels of roughness scales is best for nonwettability out of the topographies studied here. This observation may partially explain why Nature chose rounded-top protrusions, such as those on the Lotus leaf. The least useful topography is the flat-top pillars with three roughness scales. In the case of the triadic Koch topography, four roughness scales are required to have nonwettable surface.

  18. Radiation-Resistant Hybrid Lotus Effect for Achieving Photoelectrocatalytic Self-Cleaning Anticontamination Coatings

    Science.gov (United States)

    Taylor, Edward W.; Pirich, Ronald G.

    2011-01-01

    An experiment involving radiation-resistant hydrophobic coatings is planned for space exposure and experimental testing on the International Space Station (ISS) in 2011. The Lotus biocide coatings are designed for supporting space exploration missions. This innovation is an antibacterial, anti-contamination, and self-cleaning coating that uses nano-sized semiconductor semimetal oxides to neutralize biological pathogens and toxic chemicals, as well as to mitigate dust accumulation (see figure). The Lotus biocide coating is thin (approximately microns thick), lightweight, and the biocide properties will not degrade with time or exposure to biological or chemical agents. The biocide is stimulated chemically (stoichiometric reaction) through exposure to light (photocatalysis), or by an applied electric field (electrocatalysis). The hydrophobic coating samples underwent preliminary high-energy proton and alpha-ray (helium ion) irradiations at the Lawrence Berkeley National Laboratory 88" cyclotron and demonstrated excellent radiation resistance for a portion of the Galactic Cosmic Ray (GRC) and Solar Proton spectrum. The samples will undergo additional post-flight studies when returned to Earth to affirm further the radiation resistance properties of the space exposed coatings.

  19. Biomimetic approaches for green tribology: from the lotus effect to blood flow control

    Science.gov (United States)

    Maani, Nazanin; Rayz, Vitaliy S.; Nosonovsky, Michael

    2015-09-01

    The research in Green tribology combines several areas including biomimetic tribomaterials and surfaces for controlled adhesion. Biomimetic surfaces mimic living nature and thus they are eco-friendly. The most famous biomimetic surface effect is the Lotus effect (reduction of water adhesion to a solid surface due to micro/nanostructuring of the solid surface). Several extensions of the Lotus effect have been discussed in the literature including the oleophobicity (repelling organic liquids such as oils), underwater oleophobicity to reduce fouling, and the shark skin effect (flow drag reduction due to specially oriented micro-riblets). Here we suggest a potentially important application of micro/nanostructured surfaces in the biomedical area: the micro/nanostructure controlled adhesion in blood flow. Blood is a suspension, and its adhesion properties are different from those of water and oil. For many cardiovascular applications, it is desirable to reduce stagnation and clotting of blood. Therefore, both the underwater oleophobicuity and shark-skin effect can be used. We discuss how computational fluid dynamics models can be used to investigate the structure-property relationships of surface pattern-controlled blood flow adhesion.

  20. Fabrication of cylindrical superhydrophobic microchannels by replicating lotus leaf structures on internal walls

    Science.gov (United States)

    Das, Ajit; Bhaumik, Soubhik Kumar

    2018-04-01

    Cylindrical superhydrophobic microchannels are fabricated by replicating lotus leaf structures on internal walls. The fabrication process comprises of three steps: the creation of a cylindrical mold of a glass rod (125 µm) with polystyrene films bearing negative imprints of lotus leaf (superhydrophobic) structures; casting polydimethylsiloxane (PDMS, Sylgard 184) over the mold; and solvent-assisted pulling off of the glass rod to leave a positive replica on the inner wall of the PDMS cast. The last crucial step is achieved through selective dissolution of the intermediate negative replica layer in the cylindrical mold without any swelling effect. The high fidelity of the replication process is confirmed through scanning electron microscope (SEM) imaging. The attained superhydrophobicity is assessed by comparing the dynamics of the advancing meniscus in the fabricated microchannels with that over a similarly fabricated smooth microchannel. Contact angle studies of the meniscus reveal a lower capillary effect and drag force experienced by the superhydrophobic microchannel compared to smooth ones. Studies based on velocity lead to a prediction of a drag reduction of 35%. A new avenue is thus opened up for microfabrication and flow analysis of closed superhydrophobic (SH) conduits in lab on chip and microfluidic applications.

  1. Biomimetic approaches for green tribology: from the lotus effect to blood flow control

    International Nuclear Information System (INIS)

    Maani, Nazanin; Rayz, Vitaliy S; Nosonovsky, Michael

    2015-01-01

    The research in Green tribology combines several areas including biomimetic tribomaterials and surfaces for controlled adhesion. Biomimetic surfaces mimic living nature and thus they are eco-friendly. The most famous biomimetic surface effect is the Lotus effect (reduction of water adhesion to a solid surface due to micro/nanostructuring of the solid surface). Several extensions of the Lotus effect have been discussed in the literature including the oleophobicity (repelling organic liquids such as oils), underwater oleophobicity to reduce fouling, and the shark skin effect (flow drag reduction due to specially oriented micro-riblets). Here we suggest a potentially important application of micro/nanostructured surfaces in the biomedical area: the micro/nanostructure controlled adhesion in blood flow. Blood is a suspension, and its adhesion properties are different from those of water and oil. For many cardiovascular applications, it is desirable to reduce stagnation and clotting of blood. Therefore, both the underwater oleophobicuity and shark-skin effect can be used. We discuss how computational fluid dynamics models can be used to investigate the structure–property relationships of surface pattern-controlled blood flow adhesion. (paper)

  2. Nanocrystalline nickel films with lotus leaf texture for superhydrophobic and low friction surfaces

    Science.gov (United States)

    Shafiei, Mehdi; Alpas, Ahmet T.

    2009-11-01

    Nanostructured Ni films with high hardness, high hydrophobicity and low coefficient of friction (COF) were fabricated. The surface texture of lotus leaf was replicated using a cellulose acetate film, on which a nanocrystalline (NC) Ni coating with a grain size of 30 ± 4 nm was electrodeposited to obtain a self-sustaining film with a hardness of 4.42 GPa. The surface texture of the NC Ni obtained in this way featured a high density (4 × 10 3 mm -2) of conical protuberances with an average height of 10.0 ± 2.0 μm and a tip radius of 2.5 ± 0.5 μm. This structure increased the water repellency and reduced the COF, compared to smooth NC Ni surfaces. The application of a short-duration (120 s) electrodeposition process that deposited "Ni crowns" with a larger radius of 6.0 ± 0.5 μm on the protuberances, followed by a perfluoropolyether (PFPE) solution treatment succeeded in producing a surface texture consisting of nanotextured protuberances that resulted in a very high water contact angle of 156°, comparable to that of the superhydrophobic lotus leaf. Additionally, the microscale protuberances eliminated the initial high COF peaks observed when smooth NC Ni films were tested, and the PFPE treatment resulted in a 60% reduction in the steady-state COFs.

  3. Evolution of crop production under a pseudo-space environment using model plants, Lotus japonicus

    Science.gov (United States)

    Tomita-Yokotani, Kaori; Motohashi, Kyohei; Omi, Naomi; Sato, Seigo; Aoki, Toshio; Hashimoto, Hirofumi; Yamashita, Masamichi

    Habitation in outer space is one of our challenges. We have been studying space agriculture and/or spacecraft agriculture to provide food and oxygen for the habitation area in the space environment. However, careful investigation should be made concerning the results of exotic environmental effects on the endogenous production of biologically active substances in indi-vidual cultivated plants in a space environment. We have already reported that the production of functional substances in cultivated plants as crops are affected by gravity. The amounts of the main physiological substances in these plants grown under terrestrial control were different from that grown in a pseudo-microgravity. These results suggested that the nutrition would be changed in the plants/crops grown in the space environment when human beings eat in space. This estimation required us to investigate each of the useful components produced by each plant grown in the space environment. These estimations involved several study fields, includ-ing nutrition, plant physiology, etc. On the other hand, the analysis of model plant genomes has recently been remarkably advanced. Lotus japonicus, a leguminous plant, is also one of the model plant. The leguminosae is a large family in the plant vegetable kingdom and almost the entire genome sequence of Lotus japonicus has been determined. Nitrogen fixation would be possible even in a space environment. We are trying to determine the best conditions and evolution for crop production using the model plants.

  4. Lotus corniculatus Crop Growth of in Crude Oil Contaminated Soil. Part 2 Biomass Metals Bioaccumulation

    Directory of Open Access Journals (Sweden)

    Florica Morariu

    2016-05-01

    Full Text Available Phytoremediation involves the ability of plants to remove pollutants and is a promise on low costs and efficient processes for cleaning oil polluted soil. Studies for phytoremediation of soils polluted with petroleum products were critical and were based on monitoring strategies implemented efficiency. These strategies are based on the necessity of treating polluted soil and plant cultivation. Treatment was performed with recycled materials, sewage sludge as fertilizer and fly ash as amendment. The studies took on the characteristics of qualitative and quantitative of Lotus corniculatus crops, plants tolerant to conditions for phytoremediation strategy implemented on polluted soils by 80.5 ± 3.9 g·kg-1 D.M. The use of sewage sludge mixed with fly ash resulted in formation of a layer covering the surface with vegetable grown by 85 - 94 % in July and by 67 - 83 % in August. In Lotus corniculatus crops have not been registered bioaccumulation of toxic metals according to legislation from Romania.

  5. Evaluation of in vitro aldose reductase inhibitory potential of alkaloidal fractions of Piper nigrum, Murraya koenigii, Argemone mexicana, and Nelumbo nucifera.

    Science.gov (United States)

    Gupta, Sakshi; Singh, Nirmal; Jaggi, Amteshwar Singh

    2014-05-01

    Aldose reductase is primarily involved in development of long-term diabetic complications due to increased polyol pathway activity. The synthetic aldose reductase inhibitors are not very successful clinically. Therefore, the natural sources may be exploited for safer and effective aldose reductase inhibitors. In the present study, the aldose reductase inhibitory potential of hydroalcoholic and alkaloidal extracts of Piper nigrum, Murraya koenigii, Argemone mexicana, and Nelumbo nucifera was evaluated. The hydroalcoholic and alkaloidal extracts of the selected plants were prepared. The different concentrations of hydroalcoholic and alkaloidal extracts of these plants were evaluated for their goat lens aldose reductase inhibitory activity using dl-glyceraldehyde as substrate. The aldose reductase inhibitory potential of extracts was assessed in terms of their IC50 value. Amongst the hydroalcoholic extracts, the highest aldose reductase inhibitory activity was shown by P. nigrum (IC50 value 35.64±2.7 μg/mL) followed by M. koenigii (IC50 value 45.67±2.57 μg/mL), A. mexicana (IC50 value 56.66±1.30 μg/mL), and N. nucifera (IC50 value 59.78±1.32 μg/mL). Among the alkaloidal extracts, highest inhibitory activity was shown by A. mexicana (IC50 value 25.67±1.25 μg/mL), followed by N. nucifera (IC50 value 28.82±1.85 μg/mL), P. nigrum (IC50 value 30.21±1.63 μg/mL), and M. koenigii (IC50 value 35.66±1.64 μg/mL). It may be concluded that the alkaloidal extracts of these plants possess potent aldose reductase inhibitory activity and may be therapeutically exploited in diabetes-related complications associated with increased activity of aldose reductase.

  6. Nelumbo nucifera Gaertn leaves extract inhibits the angiogenesis and metastasis of breast cancer cells by downregulation connective tissue growth factor (CTGF) mediated PI3K/AKT/ERK signaling.

    Science.gov (United States)

    Chang, Chun-Hua; Ou, Ting-Tsz; Yang, Mon-Yuan; Huang, Chi-Chou; Wang, Chau-Jong

    2016-07-21

    Nelumbo nucifera Gaertn (Nymphaeaceae) has been recognized as a medicinal plant, which was distributed throughout the Asia. The aqueous extract of Nelumbo nucifera leaves extract (NLE) has various biologically active components such as polyphenols, flavonoids, oligomeric procyanidines. However, the role of NLE in breast cancer therapy is poorly understood. The purpose of this study was to identify the hypothesis that NLE can suppress tumor angiogenesis and metastasis through CTGF (connective tissue growth factor), which has been implicated in tumor angiogenesis and progression in breast cancer MDA-MB-231 cells. We examined the effects of NLE on angiogenesis in the chicken chorioallantoic membrane (CAM) model. The data showed that NLE could reduce the chorionic plexus at day 17 in CAM and the duration of this inhibition was dose-dependent. In Xenograft model, NLE treatment significantly reduced tumor weight and CD31 (capillary density) over control, respectively. We examined the role of angiogenesis involved restructuring of endothelium using human umbilical vein endothelial cell (HUVEC) in Matrigel angiogenesis model. The results indicated that vascular-like structure formation was further blocked by NLE treatment. Moreover, knockdown of CTGF expression markedly reduced the expression of MMP2 as well as VEGF, and attenuated PI3K-AKT-ERK activation, indication that these signaling pathways are crucial in mediating CTGF function. The present results suggest that NLE might be useful for treatment in therapy-resistance triple negative breast cancer. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  7. Identification of QTLs for shoot and root growth under ionic-osmotic stress in Lotus, using a RIL population

    DEFF Research Database (Denmark)

    Quero, Gastón; Gutíerrez, Lucía; Lascano, Ramiro

    2014-01-01

    The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic-osmotic stress in a population of recombinant inbred lines (RILs...

  8. CAROTENOID CLEAVAGE DIOXYGENASE 7 modulates plant growth, reproduction, senescence, and determinate nodulation in the model legume Lotus japonicas

    NARCIS (Netherlands)

    Liu, J.; Novero, M.; Charnikhova, T.; Ferrandino, A.; Schubert, A.; Ruyter-Spira, C.P.; Biofante, P.; Lovisolo, C.; Bouwmeester, H.J.

    2013-01-01

    Strigolactones (SLs) are newly identified hormones that regulate multiple aspects of plant development, infection by parasitic weeds, and mutualistic symbiosis in the roots. In this study, the role of SLs was studied for the first time in the model plant Lotus japonicus using transgenic lines

  9. Organization and expression of genes in the genomic region surrounding the glutamine synthetase gene Gln1 from Lotus japonicus

    DEFF Research Database (Denmark)

    Thykjaer, T; Danielsen, D; She, Q

    1997-01-01

    The diploid Lotus japonicus was previously suggested as a model for the legume plant family. We present here the nucleotide sequence and the derived gene organization of a small part of the genome in this model plant. Two functional genes with the same transcriptional orientation were identified...

  10. Comparative ionomics and metabolomics in extremophile and glycophytic Lotus species under salt stress challenge the metabolic pre-adaptation hypothesis.

    Science.gov (United States)

    Sanchez, Diego H; Pieckenstain, Fernando L; Escaray, Francisco; Erban, Alexander; Kraemer, Ute; Udvardi, Michael K; Kopka, Joachim

    2011-04-01

    The legume genus Lotus includes glycophytic forage crops and other species adapted to extreme environments, such as saline soils. Understanding salt tolerance mechanisms will contribute to the discovery of new traits which may enhance the breeding efforts towards improved performance of legumes in marginal agricultural environments. Here, we used a combination of ionomic and gas chromatography-mass spectrometry (GC-MS)-based metabolite profilings of complete shoots (pooling leaves, petioles and stems) to compare the extremophile Lotus creticus, adapted to highly saline coastal regions, and two cultivated glycophytic grassland forage species, Lotus corniculatus and Lotus tenuis. L. creticus exhibited better survival after exposure to long-term lethal salinity and was more efficient at excluding Cl⁻ from the shoots than the glycophytes. In contrast, Na+ levels were higher in the extremophile under both control and salt stress, a trait often observed in halophytes. Ionomics demonstrated a differential rearrangement of shoot nutrient levels in the extremophile upon salt exposure. Metabolite profiling showed that responses to NaCl in L. creticus shoots were globally similar to those of the glycophytes, providing little evidence for metabolic pre-adaptation to salinity. This study is the first comparing salt acclimation responses between extremophile and non-extremophile legumes, and challenges the generalization of the metabolic salt pre-adaptation hypothesis. © 2011 Blackwell Publishing Ltd.

  11. Better anatomical and cosmetic results using tunneled lotus petal flap for plastic reconstruction after demolitive surgery for vulvar malignancy.

    Science.gov (United States)

    Buda, Alessandro; Confalonieri, Pier Luigi; Rovati, Luca Carlo Vittorio; Fruscio, Robert; Giuliani, Daniela; Signorelli, Mauro; Dell'Anna, Tiziana; Pirovano, Cecilia; Milani, Rodolfo

    2012-06-01

    To evaluate the efficacy of tunneled lotus petal flap in terms of anatomical and cosmetic results in patients who underwent vulvoperineal reconstruction for vulvar malignancy. Between March 2010 and July 2011, 22 women underwent vulvoperineal reconstruction using tunneled lotus petal flap for primary or recurrent disease at San Gerardo Hospital, Monza. In 16 cases, lotus flaps were bilateral, whereas in 6 cases, they were monolateral. The median age was 72 years (range, 53-87 years). The mean operating time was 85 minutes. The mean length of follow-up was 10 months (range, 2-16 months). Postoperative complications occurred in 2 patients, including one case of partial flap necrosis and one case of donor site breakdown. Tunneled lotus petal flap is safe, easy and fast to perform, has a low rate of complications, and good functional and cosmetic results. This technique represents an optimal solution for plastic reconstruction in case of primary or recurrent vulvar disease, or in case of introital stenosis after primary inadequate closure.

  12. A Summary of Aquatic Vegetation Monitoring at Selected Locations in Pools 4, 8, 13, and 26 and La Grange Pool of the Upper Mississippi River System. 1993 Annual Status Report,

    Science.gov (United States)

    1998-07-01

    Najas guadalupensis (Spreng.) Magnus Nymphaeaceae American lotus Nelumbo lutea (Willd.) Pers. Nymphaeaceae White waterlily Nymphaea odorata Ait... Nymphaeaceae Yellow pondlily Nuphar lutea (L.) Sm Onagraceae Floating primrosewillow Jussiaea repens L. Pontederiaceae Water stargrass, Heteranthera dubia

  13. Sacred spaces in public places: religious and spiritual plurality in health care.

    Science.gov (United States)

    Reimer-Kirkham, Sheryl; Sharma, Sonya; Pesut, Barb; Sawatzky, Richard; Meyerhoff, Heather; Cochrane, Marie

    2012-09-01

    Several intriguing developments mark the role and expression of religion and spirituality in society in recent years. In what were deemed secular societies, flows of increased sacralization (variously referred to as 'new', 'alternative', 'emergent' and 'progressive' spiritualities) and resurgent globalizing religions (sometimes with fundamentalist expressions) are resulting in unprecedented plurality. These shifts are occurring in conjunction with increasing ethnic diversity associated with global migration, as well as other axes of difference within contemporary society. Democratic secular nations such as Canada are challenged to achieve social cohesion in the face of growing religious, spiritual and ethnic diversity. These challenges are evident in the high-paced, demanding arena of Health care. Here, religious and spiritual plurality enter in, sometimes resulting in conflict between medical services and patients' beliefs, other times provoking uncertainties on the part of healthcare professionals about what to do with their own religiously or spiritually grounded values and beliefs. In this paper, we present selected findings from a 3-year study that examined the negotiation of religious and spiritual pluralism in Health care. Our focus is on the themes of 'sacred' and 'place', exploring how the sacred - that which is attributed as special and set apart as it pertains to the divine, transcendence, God or higher power - takes form in social and material spaces in hospitals. © 2011 Blackwell Publishing Ltd.

  14. Compassionate love as a mechanism linking sacred qualities of marriage to older couples' marital satisfaction.

    Science.gov (United States)

    Sabey, Allen K; Rauer, Amy J; Jensen, Jakob F

    2014-10-01

    Previous work has underscored the robust links between sanctification of marriage and marital outcomes, and recent developments in the literature suggest that compassionate love, which is important for intimate relationships, may act as a mediator of that relationship. Accordingly, the current study used actor-partner interdependence models to examine the relationship between a spiritual cognition (i.e., perceived sacred qualities of marriage) and marital satisfaction, and to determine whether that relationship is mediated by compassionate love, in a sample of older married couples (N = 64). Results revealed that wives' greater sacred qualities of marriage were significantly and positively linked to marital satisfaction on the part of both spouses, and that these links were partially mediated by couples' reports of compassionate love. These findings highlight the importance of moving beyond simply establishing the existence of the link between global markers of involvement of religion and marriage to understanding how specific spiritual cognitions may foster better relationship quality, especially among older couples. (PsycINFO Database Record (c) 2014 APA, all rights reserved).

  15. First in human implantation of the mechanical expanding Lotus® valve in degenerated surgical valves in mitral position.

    Science.gov (United States)

    Schaefer, Ulrich; Conradi, Lenard; Lubos, Edith; Deuschl, Florian; Schofer, Niklas; Seiffert, Moritz; Treede, Hendrik; Schirmer, Johannes; Reichenspurner, Hermann; Blankenberg, Stefan

    2015-12-01

    Implantation of transcatheter heart valves (THV) into degenerated surgical valves is an emerging therapy for selected high-risk patients. Although, CE mark of most THV is limited for native aortic valvular stenosis, transcatheter valve implantation into degenerated bioprostheses, even in mitral position is very intriguing. After placement of a cerebral protection device (Claret Sentinel®), three consecutive patients (age, 79.0 ± 6.1; log EuroSCORE I: 33.3 ± 9.2%) with a degenerated mitral bioprosthesis were treated by transapical implantation of the Lotus® valve (Boston Scientific Inc.). In addition, a SwanGanz catheter was introduced in the pulmonary artery for hemodynamic assessments all patients. Procedural success was 100%. Valve implantation was performed without rapid ventricular pacing. Resheating was performed in two patients due to suboptimal initial positioning. Invasive online hemodynamics revealed stable blood pressure in all patients. After Lotus® valve implantation, valvular mitral regurgitation was completely eliminated in all patients. One patient had a mild paravalvular leak of the surgical bioprosthesis, which was present before implantation. Invasive right and left heart hemodynamics showed an immediate improvement after Lotus® valve implantation. Mean mitral surface area (2.1 ± 0.2 cm(2) ) and mean gradient (3.7 ± 2.1 mm Hg) demonstrated satisfactory results. All patients were immediately extubated and discharged from the hospital without any adverse event. This study demonstrates for the first time the feasibility of transapical Lotus® Valve implantation in degenerated mitral bioprostheses. The controlled mechanical Lotus® valve expansion with remarkably stable hemodynamics throughout the procedure offers a new and valuable treatment option. © 2015 Wiley Periodicals, Inc.

  16. Long-Term follow up after intra-Uterine transfusionS; the LOTUS study.

    Science.gov (United States)

    Verduin, Esther P; Lindenburg, Irene T M; Smits-Wintjens, Vivianne E H J; van Klink, Jeanine M M; Schonewille, Henk; van Kamp, Inge L; Oepkes, Dick; Walther, Frans J; Kanhai, Humphrey H H; Doxiadis, Ilias I N; Lopriore, Enrico; Brand, Anneke

    2010-12-01

    The Leiden University Medical Center (LUMC) is the Dutch national referral centre for pregnancies complicated by haemolytic disease of the fetus and newborn (HDFN) caused by maternal alloimmunization. Yearly, 20-25 affected fetuses with severe anaemia are transfused with intra-uterine blood transfusions (IUT). Mothers of whom their fetus has undergone IUT for HDFN are considered high responders with regard to red blood cell (RBC) antibody formation. Most study groups report high perinatal survival, resulting in a shift in attention towards short- and long-term outcome in surviving children. We set up a large long-term observational follow-up study (LOTUS study), in cooperation with the Sanquin Blood Supply Foundation and the LUMC departments of Obstetrics, Neonatology and ImmunoHematology & Bloodtransfusion.The first part of this study addresses several putative mechanisms associated with blood group alloimmunization in these mothers. The second part of this study determines the incidence of long-term neurodevelopment impairment (NDI) and associated risk factors in children treated with IUT. All women and their life offspring who have been treated with IUT for HDFN in the LUMC from 1987-2008 are invited to participate and after consent, blood or saliva samples are taken. RBC and HLA antigen profile and antibodies are determined by serologic or molecular techniques. Microchimerism populations are tested by real time polymerase chain reaction (RT PCR).All children are tested for their neurological, cognitive and psychosocial development using standardised tests and questionnaires. The primary outcome is neurodevelopmental impairment (NDI), a composite outcome defined as any of the following: cerebral palsy, cognitive or psychomotor development blindness and/or bilateral deafness. The LOTUS study includes the largest cohort of IUT patients ever studied and is the first to investigate post-IUT long-term effects in both mother and child. The results may lead to a change

  17. Long-Term follow up after intra-Uterine transfusionS; the LOTUS study

    Directory of Open Access Journals (Sweden)

    Kanhai Humphrey HH

    2010-12-01

    Full Text Available Abstract Background The Leiden University Medical Center (LUMC is the Dutch national referral centre for pregnancies complicated by haemolytic disease of the fetus and newborn (HDFN caused by maternal alloimmunization. Yearly, 20-25 affected fetuses with severe anaemia are transfused with intra-uterine blood transfusions (IUT. Mothers of whom their fetus has undergone IUT for HDFN are considered high responders with regard to red blood cell (RBC antibody formation. Most study groups report high perinatal survival, resulting in a shift in attention towards short- and long-term outcome in surviving children. Methods/Design We set up a large long-term observational follow-up study (LOTUS study, in cooperation with the Sanquin Blood Supply Foundation and the LUMC departments of Obstetrics, Neonatology and ImmunoHematology & Bloodtransfusion. The first part of this study addresses several putative mechanisms associated with blood group alloimmunization in these mothers. The second part of this study determines the incidence of long-term neurodevelopment impairment (NDI and associated risk factors in children treated with IUT. All women and their life offspring who have been treated with IUT for HDFN in the LUMC from 1987-2008 are invited to participate and after consent, blood or saliva samples are taken. RBC and HLA antigen profile and antibodies are determined by serologic or molecular techniques. Microchimerism populations are tested by real time polymerase chain reaction (RT PCR. All children are tested for their neurological, cognitive and psychosocial development using standardised tests and questionnaires. The primary outcome is neurodevelopmental impairment (NDI, a composite outcome defined as any of the following: cerebral palsy, cognitive or psychomotor development Discussion The LOTUS study includes the largest cohort of IUT patients ever studied and is the first to investigate post-IUT long-term effects in both mother and child. The

  18. Mirroring the Object of the Lesson: The Creative Process of Scriptural Rewriting as an Effective Practice for Teaching Sacred Texts

    Science.gov (United States)

    Palmer, Carmen

    2018-01-01

    This paper introduces Rewritten Scripture and scriptural rewriting as a creative process that, when mirrored in a teaching exercise, may serve as an effective practice in teaching sacred texts. Observing changes made between scripture and its rewriting may allow readers to identify different contexts among these texts. Furthermore, the act of…

  19. THE BIOLOGICAL VALUES AND CONSERVATION STATUS OF SACRED GROVES IN THE BALASORE WILDLIFE DIVISION, ODISHA: A CASE STUDY

    Directory of Open Access Journals (Sweden)

    Raj Kishore MOHANTA

    2012-09-01

    Full Text Available On a global scale, the existing Sacred Groves (SGs are based on ancestral worship and focus on the conservation of forest patches. Sacred groves are distributed over a wide ecosystem and help in the conservation of rare and endemic species. Well preserved sites are store houses of biological, ecological, medicinal, ethno-cultural and religious values. We documented the state of 13 Sacred Groves in Balasore, Odisha during March 2011. For a detailed investigation, sample areas were set, for the assessment of floral and faunal diversity, ethno-cultural values and management status. A total of 58 floral species and 13 faunal species were recorded. In Balasore, Sacred Groves are small in size and can act as starting points for any long term conservation plan of biodiversity. The communities have kept their faith and traditions linked to these mini nuclei of rich biodiversity in the landscape. Therefore, any conservation program can begin from local communities, by taking them into consideration as trustworthy awareness building factors.

  20. Treasures in Heaven and on Earth : Poverty and Abundance in the Life and Sacred Cantatas of Johann Sebastian Bach

    NARCIS (Netherlands)

    Heber, N.

    2017-01-01

    This PhD dissertation explores poverty and abundance in Bach’s life and sacred cantatas and inquires how he himself might have handled the tension between the material and spiritual aspects. Although his career was increasingly lucrative, Bach did not amass significant wealth. In 1730, he complained

  1. Establishing and screening of a Lotus japonicus LORE1 mutagenized population

    DEFF Research Database (Denmark)

    Malolepszy, Anna

    incomplete and we are therefore unable to fully understand the symbiotic process. This highlights the necessity for new mutagenesis and gene function tools for further investigation of the genetic mechanisms governing the symbiotic process. This work demonstrates that LORE1 can be successfully used for high......-throughput insertional mutagenesis of Lotus and that a large mutant population can be managed. Lack of insertional hotspots and a frequency of 2,4 new insertions per line, makes it an attractive tool to work with and gives the possibility to obtain cost-effective genome saturation (Urbanski et al., 2012). Additionally......, LORE1 can be successfully used for forward screening and discovery of genes involved in the nodulation process. Since LORE1 is mostly active in pollen, each plant is carrying independent insertions. Taking advantage of this feature and supporting the reliability of the large-scale set-up, well...

  2. On-line tritium production and heat deposition rate measurements at the Lotus facility

    International Nuclear Information System (INIS)

    Joneja, O.P.; Scherrer, P.; Anand, R.P.

    1994-01-01

    Integral tritium production and heat deposition measurement in a prototype fusion blanket would enable verification of the computational codes and the data based employed for the calculations. A large number of tritium production rate measurements have been reported for different type of blankets, whereas the direct heat deposition due to the mixed radiation field in the fusion environment, is still in its infancy. In order to ascertain the kerma factors and the photon production libraries, suitable techniques must be developed to directly measure the nuclear heat deposition rates in the materials required for the fusion systems. In this context, at the Lotus facility, we have developed an extremely efficient double ionizing chamber, for the on-line tritium production measurements and employed a pure graphite calorimeter to measure the nuclear heat deposition due to the mixed radiation field of the 14 MeV, Haefely neutron generator. This paper presents both systems and some of the recent measurements. (authors). 8 refs., 13 figs

  3. Mimicking the lotus effect: influence of double roughness structures and slender pillars.

    Science.gov (United States)

    Patankar, Neelesh A

    2004-09-14

    Surface roughness is known to amplify hydrophobicity. The apparent contact angle of a drop on a rough surface is often modeled using either Wenzel's or Cassie's formulas. These formulas, along with an appropriate energy analysis, are critical in designing superhydrophobic substrates for applications in microscale devices. In this paper we propose that double (or multiple) roughness structures or slender pillars are appropriate surface geometries to develop "self-cleaning" surfaces. The key motivation behind the double structured roughness is to mimic the microstructure of superhydrophobic leaves (such as lotus). Theoretical analysis similar to that presented in the paper can be used to obtain optimal geometric parameters for the rough surface. The calculation procedure should result in surface geometries with excellent water repellent properties.

  4. Proteome analysis of pod and seed development in the model legume Lotus japonicus

    DEFF Research Database (Denmark)

    Nautrup-Pedersen, G.; Dam, S.; Laursen, B. S.

    2010-01-01

    of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965......Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses...... and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate...

  5. Global changes in transcription orchestrate metabolic differentiation during symbiotic nitrogen fixation in Lotus japonicus

    DEFF Research Database (Denmark)

    Colebatch, Gillian; Desbrosses, Guilhem; Ott, Thomas

    2004-01-01

    from specific sets of induced genes. In addition to the expected signs of hypoxia, numerous indications were obtained that nodule cells also experience P-limitation and osmotic stress. Several potential regulators of these stress responses were identified. Metabolite profiling by gas chromatography......Research on legume nodule metabolism has contributed greatly to our knowledge of primary carbon and nitrogen metabolism in plants in general, and in symbiotic nitrogen fixation in particular. However, most previous studies focused on one or a few genes/enzymes involved in selected metabolic...... pathways in many different legume species. We utilized the tools of transcriptomics and metabolomics to obtain an unprecedented overview of the metabolic differentiation that results from nodule development in the model legume, Lotus japonicus. Using an array of more than 5000 nodule cDNA clones...

  6. Robust Superhydrophobic Carbon Nanotube Film with Lotus Leaf Mimetic Multiscale Hierarchical Structures.

    Science.gov (United States)

    Wang, Pengwei; Zhao, Tianyi; Bian, Ruixin; Wang, Guangyan; Liu, Huan

    2017-12-26

    Superhydrophobic carbon nanotube (CNT) films have demonstrated many fascinating performances in versatile applications, especially for those involving solid/liquid interfacial processes, because of their ability to affect the material/energy transfer at interfaces. Thus, developing superhydrophobic CNTs has attracted extensive research interests in the past decades, and it could be achieved either by surface coating of low-free energy materials or by constructing micro/nanohierarchical structures via various complicated processes. So far, developing a simple approach to fabricate stable superhydrophobic CNTs remains a challenge because the capillary force induced coalescence frequently happens when interacting with liquid. Herein, drawing inspirations from the lotus leaf, we proposed a simple one-step chemical vapor deposition approach with programmable controlled gas flow to directly fabricate a CNT film with rather stable superhydrophobicity, which can effectively prevent even small water droplets from permeating into the film. The robust superhydrophobicity was attributable to typical lotus-leaf-like micro/nanoscale hierarchical surface structures of the CNT film, where many microscale clusters composed of entangled nanotubes randomly protrude out of the under-layer aligned nanotubes. Consequently, dual-scale air pockets were trapped within each microscale CNT cluster and between, which could largely reduce the liquid/solid interface, leading to a Cassie state. Moreover, the superhydrophobicity of the CNT film showed excellent durability after long time exposure to air and even to corrosive liquids with a wide range of pH values. We envision that the approach developed is advantageous for versatile physicochemical interfacial processes, such as drag reduction, electrochemical catalysis, anti-icing, and biosensors.

  7. K-targeted strategy for isolation of phenolic alkaloids of Nelumbo nucifera Gaertn by counter-current chromatography using lysine as a pH regulator.

    Science.gov (United States)

    Wang, Yanyan; Zhang, Lihong; Zhou, Hui; Guo, Xiuyun; Wu, Shihua

    2017-03-24

    Counter-current chromatography (CCC) is an efficient liquid-liquid partition chromatography technique without support matrix. Despite there are many significant advancements in the CCC separation of natural products especially for non-ionic neutral compounds, CCC isolation of ionic compounds including alkaloids is still a challenging process guide by classical partition coefficients (K) or distribution ratio (K C ) because their partition coefficient could not be equal to distribution ratio in common ionic conditions. Here, taking the extract of embryo of the seed of Nelumbo nucifera Gaertn as sample, we introduced a modified K-targeted strategy for isolation of phenolic alkaloids by use of lysine as a pH regulator. The results indicated that if the mass of basic regulators such as aqueous ammonia and lysine added into the solvent system were high enough to inhibit the ionization of the targeted alkaloids, the distribution ratio of targets with ionic and non-ionic molecular forms got stable and might not been changed as the concentration of the pH regulator. In this case, the distribution ratio of target was almost equal to the partition coefficient. Thus, the targets could be isolated by K-targeted CCC separation through adding a certain amount pH regulators into the solvent system. Further experiments also showed that the sample concentration was an important factor on the distribution ratio of targets. Meanwhile, CCC experiments indicated that lysine was more suitable than aqueous ammonia for the separation of phenolic alkaloids because the chemical property of lysine-target complex in the CCC fractions was more stable. Therefore, the preparative CCC separation was performed using 20mM lysine as a pH regulator with more than 800mg injection mass. After simple back-extraction with dichloromethane, the lysine in the CCC fraction was removed completely and pure isoliensinine and neferine were obtained. In summary, the whole results indicated that the modified K

  8. The Role of Wali, Ancient Mosques and Sacred Tombs in the Dynamics of Islamisation in Lombok

    Directory of Open Access Journals (Sweden)

    Erni Budiwanti

    2015-02-01

    Full Text Available This paper highlights specifically the inter-relationship amidst the Wali, ancient mosques and sacred tombs (makam playing vital role in the process of Islamization in the Indonesian archipelago, and in Lombok in particular. The process of converting local religion to Islam involves Wali - a venerated religious figure coming from outside Lombok- and the tangible legacies that he left in the forms of keramat creating new tradition of ziarah. The early stage of conversion was strongly marked by contextualizing Islam into local cultural symbol and cosmology. Embedded in this stage is the development of venerated attributes attached to keWalian, in the forms of: karamah, ngalap berkah, and wasilah. These spiritual attributes are pinpointed to highlight a dis¬tinctive cultural variance of Sasak Muslims in Lombok.

  9. Sacred Psychotherapy in the “Age of Authenticity”: Healing and Cultural Revivalism in Contemporary Finland

    Directory of Open Access Journals (Sweden)

    James M. Wilce

    2011-10-01

    Full Text Available Like other European countries, contemporary Finland has witnessed an explosion of healing modalities designatable as “New Age” (though not without profound controversy, [1]. This paper focuses on Finnish courses in lament (wept song, tuneful weeping with words that combine healing conceived along psychotherapeutic lines and lessons from the lament tradition of rural Karelia, a region some Finns regard as their cultural heartland. A primary goal of the paper is to explicate a concept of “authenticity” emerging in lament courses, in which disclosing the depths of one’s feelings is supported not only by invoking “psy-“ discourses of self-help, but also by construing the genuine emotional self-disclosure that characterizes neolamentation as a sacred activity and a vital contribution to the welfare of the Finnish people.

  10. To have and to hold: possessing the sacred in the late renaissance

    Directory of Open Access Journals (Sweden)

    Erin E. Benay

    2015-02-01

    Full Text Available Private devotional art of the early 17th century often found its place in the galleries of noblemen and women whose diverse collections were symbols not only of stylistic taste, but of their owners’ exhaustive curiosity. In these domestic settings, boundaries between sacred and secular were permeable, as the unprecedented physical intimacy portrayed in popular religious subjects such as St Matthew and the Angel, the Stigmatisation of St Francis, or Christ’s Agony in the Garden reveal. Representations of the latter reminded viewers of Christ’s human, corporal suffering and suggested a model of resolve strengthened by prayer. The Agony in the Garden appears on the interior of Jacopo Ligozzi’s virtuosic Portable Altar with Carrying Case (1608, likely a Medici gift presented to the Austrian court in anticipation of the marriage of archduchess Maria Maddalena to soon-to-be grand duke Cosimo II. Adorned with lavish botanical motifs on its exterior, the Altar’s potency as a sacred possession was redoubled by the owner’s tactile revelation of the portrayal of Christ supported by an Angel contained inside the case. Comprised of wood, oil on copper, and pietre dure inlay, it is an object intended to be held, opened, and experienced. This paper suggests that Ligozzi’s selective combination of sumptuous materials and choice of subject matter – botanical illustration and Christological iconography – allowed the object to appeal to the full sensorium, and therefore to function as efficaciously as a devotional aid as it did as a curiosity among other rare collectibles.

  11. Sacred byzantine music and its influence on old East Slavic Orthodox music

    Directory of Open Access Journals (Sweden)

    Włodzimierz Wołosiuk

    2014-11-01

    Full Text Available Sacred Byzantine music originates from three sources: “the liturgy of heaven”, synagogue music as well as old Greek theory of music and lays at the bottom of the East Slavs liturgical chant. The tonal base of the Byzantine music formed tetrachords. From them the so called Diatonic mode took shape. It was the easiest and the most popular sound arrangement steming from Greek music. The Cristian Church considered it to be in accordance with its Spirit and needs. From the tetrachords mentioned above other tones were created, namely Doric tones, Lydian, Phrygian and Mixolydian and, together withall their derivatives they gave beginning to the Oktoechos tradition. Byzantine music was flourishing in monasteries and in town areas andmany different forms were elaborated on like troparions, kontakions, stichiry, canons, etc. If one speaks about composers then certainly some names cannot be omitted. These are: St. Anatolius (Patriarchof Constantinople, St. Andrew of Crete, St. Romanos the Melodist,St. Sophronius of Jerusalem and, above all, St. John of Damascus who collected and systematized the liturgical chants creating mentioned Oktoechos. The acceptance of the Greek form of Christianity by Rus’ caused a cultivation of the sacred Greek vocal art on its territory which manifested in a form of so called Znamenny chant. This type of chant was at first similar to the Greek model but later on it moved away from it. Musical notation of the Old East Slavic singing was based on neumes which names in Old East Slavic have changed a little and only few survived. Furthermore, liturgical note books together with their genre and music content have been taken over from Byzantium. Especially visible in the Old East Slavic monody, Byzantine patterns were pervading also the later polyphony which proves they were always current. Moreover, this allows to claim that Rus’ became the real successor of the Greek Orthodox traditions in new circumstances of sacral

  12. The trehalose utilization gene thuA ortholog in Mesorhizobium loti does not influence competitiveness for nodulation on Lotus spp.

    Science.gov (United States)

    Ampomah, Osei Yaw; Jensen, John Beck

    2014-03-01

    Competitiveness for nodulation is a desirable trait in rhizobia strains used as inoculant. In Sinorhizobium meliloti 1021 mutation in either of the trehalose utilization genes thuA or thuB influences its competitiveness for root colonization and nodule occupancy depending on the interacting host. We have therefore investigated whether mutation in the thuA ortholog in Mesorhizobium loti MAFF303099 also leads to a similar competitive phenotype on its hosts. The results show that M. loti thuA mutant Ml7023 was symbiotically effective and was as competitive as the wild type in colonization and nodule occupancy on Lotus corniculatus and Lotus japonicus. The thuA gene in M. loti was not induced during root colonization or in the infection threads unlike in S. meliloti, despite its induction by trehalose and high osmolarity in in vitro assays.

  13. Creation of "Rose Petal" and "Lotus Leaf" Effects on Alumina by Surface Functionalization and Metal-Ion Coordination.

    Science.gov (United States)

    Mukhopadhyay, Rahul Dev; Vedhanarayanan, Balaraman; Ajayaghosh, Ayyappanpillai

    2017-12-11

    Functional differences between superhydrophobic surfaces, such as lotus leaf and rose petals, are due to the subtle architectural features created by nature. Mimicry of these surfaces with synthetic molecules continues to be fascinating as well as challenging. Herein, we demonstrate how inherently hydrophilic alumina surface can be modified to give two distinct superhydrophobic behaviors. Functionalization of alumina with an organic ligand resulted in a rose-petal-like surface (water pinning) with a contact angle of 145° and a high contact angle hysteresis (±69°). Subsequent interaction of the ligand with Zn 2+ resulted in a lotus-leaf-like surface with water rolling behavior owing to high contact angle (165°) and low-contact-angle-hysteresis (±2°). In both cases, coating of an aromatic bis-aldehyde with alkoxy chain substituents was necessary to emulate the nanowaxy cuticular feature of natural superhydrophobic materials. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. ACTIVITE ANTIMICROBIENNE ET ANTIOXYDANTE D’EXTRAIT AQUEUX DE FRUIT DE Zyziphus lotus.L. DE LA REGION TAOUNATE

    OpenAIRE

    S. LAIRINI; R.BOUSLAMTI; F. ZERROUQ; A. FARAH

    2015-01-01

    In Morocco, Zyziphus lotus.L. is widely used in traditional edicine plant. It is mainly harvested in the region Taounate. This study aims to evaluate the antimicrobial activity of an aqueous extract prepared from the fruit of this plant on Staphylococcus aureus, Listeria monocytogenes, E. Coli, Penicillium expansum and Candidum geotricum diffusion on the one hand, and analyze both the rate of total polyphenols by the Folin-Ciocalteu's and the antioxidant power of diphenylpicrylhydrazyl (DPPH)...

  15. Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

    OpenAIRE

    Zgadzaj, Rafal; Garrido-Oter, Ruben; Jensen, Dorthe Bodker; Koprivova, Anna; Schulze-Lefert, Paul; Radutoiu, Simona

    2016-01-01

    Legumes are known as pioneer plants colonizing marginal soils, and as enhancers of the nutritional status in cultivated soils. This beneficial activity has been explained by their capacity to engage in symbiotic relationship with nitrogen-fixing rhizobia. We performed a community profiling analysis of Lotus japonicus wild type and mutants to investigate the role of the nodulation pathway on the structure of the root-associated bacterial microbiota. We found that several bacterial orders were ...

  16. Ectopic expression of miR156 represses nodulation and causes morphological and developmental changes in Lotus japonicus

    OpenAIRE

    Wang, Ying; Wang, Zhishuo; Amyot, Lisa; Tian, Lining; Xu, Ziqin; Gruber, Margaret Y.; Hannoufa, Abdelali

    2014-01-01

    The effects of microRNA156 overexpression on general plant architecture, branching, flowering time and nodulation were investigated in the model legume, Lotus japonicus. We cloned an miR156 homolog, LjmiR156a, from L. japonicus, and investigated its SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes and its biological function at enhancing vegetative biomass yield, extending flowering time, and its impact on nodulation. Thirteen potential targets for LjmiR156 were identified in vitro and thei...

  17. Localisation of threat substances in urban society - LOTUS: a viable tool for finding illegal bomb factories in cities

    Science.gov (United States)

    Önnerud, Hans; Wallin, Sara; Östmark, Henric; Menning, Dennis; Ek, Stefan; Ellis, Hanna; Kölhed, Malin

    2011-06-01

    Results of dispersion experiments and dispersion modelling of explosives, drugs, and their precursors will be presented. The dispersion of chemicals evolving during preparation of home made explosives and a drug produced in an improvised manner in an ordinary kitchen has been measured. Experiments with concentration of hydrogen peroxide have been performed during spring and summer of 2009 and 2010 and further experiments with concentration of hydrogen peroxide, synthesis and drying of TATP and Methamphetamine are planned for the spring and summer of 2011. Results from the experiments are compared to dispersion modelling to achieve a better understanding of the dispersion processes and the resulting substances and amounts available for detection outside the kitchen at distances of 10-30 m and longer. Typical concentration levels have been determined as a function of environmental conditions. The experiments and modelling are made as a part of the LOTUS project aimed at detecting and locating the illicit production of explosives and drugs in an urban environment. It can be concluded that the proposed LOTUS system concept, using mobile automatic sensors, data transfer, location via GSM/GPS for on-line detection of illicit production of explosive or precursors to explosives and drugs is a viable approach and is in accordance with historical and today's illicit bomb manufacturing. The overall objective and approach of the LOTUS project will also be presented together with two more projects called PREVAIL and EMPHASIS both aiming at hindering or finding illicit production of home made explosives.

  18. The belives on the asphalt. Sacred sites as means of appropriation of the public space in México City

    Directory of Open Access Journals (Sweden)

    María Ana Portal

    2009-07-01

    Full Text Available This paper explores how residents of México City appropriate public spaces by transforming local places into sacred sites.  This sacred quality is achieved by installing crucifixes, altars and niches for virgins and saints.  These symbols of folk religion not only adorn public sites, but also offer protection, serve as mnemonic devices and manage spatial liminality -all this in a chaotic city where public spaces are considered to be almost extinct.  These elements -- faces, proper names, common religious symbols- mark “anonymous” urban spaces, generating meaning and memory for inhabitants.  In this fashion, public spaces become a scenario for projecting personal elements of  neighborhood groups.

  19. La nuit sacrée: figurações de espelhamento no filme de Nicolas Klotz

    Directory of Open Access Journals (Sweden)

    Reto Melchior

    2001-06-01

    Full Text Available O objetivo deste artigo consiste em examinar a transposição intersemiótica de algumas figurações de espelhamento no filme de Nicolas Klotz, La nuit sacrée (1993, baseado nos dois romances L 'enfant de sable (1985 et La nuit sacrée (1987, do autor marroquino Tahar Ben Jelloun, e. em salientar às distorções resultantes desta transmutação. Baseado, em primeiro lugar, na teoria semiótica formulada por Ignacio Assis Silva, em Figurativização e metamorfose: o mito de Narciso, tentei analisar a integração dos constituintes narrativos: vida - paixão - morte e metamorfose, nos dois textos literários e na adaptação cinematográfica.

  20. BETWEEN SACRED AND PROFANE: WOMAN‟S SEARCH FOR EXISTENCE IN ELIZABETH GILBERT‟S EAT, PRAY, LOVE

    Directory of Open Access Journals (Sweden)

    Fatma Hetami

    2017-04-01

    The results indicate that the meaning of profane and sacred can be revealed through the description of liz‘s process in searching her existence. It can be seen in the novel‘s text through it‘s main signifier as represented by two words ‖big house‖ and ‖bath room‖. ‖Big house‖ symbolizes happiness, peace, and power; meanwhile ‖bath room‖ symbolizes sadness and loneliness. Liz, who is supposed to be comfortable living in happiness finally decides to negate it. Instead of enjoying her wonderful life represented by wealth, marriage, and family, Liz prefers to become nomadic, divorce her husband, and have no children. The process of searching her existence then leads her into spiritual experience that can be broken down into two binary oppositions: profane and sacred. She sacralizes the profane activities such as crying in the bath room, contemplating, scrubing the temple floors, and experiencing metaphysical crisis, to reach the God.

  1. Étude de la diversité floristique par strates de quelques bois sacrés ...

    African Journals Online (AJOL)

    SARAH

    30 sept. 2013 ... fait, le gouvernement béninois à traves la Direction. Générale des Forêts et Ressources Naturelles. (DGFRN) et le Programme des Nations Unies pour le Développement (PNUD) ont initié en 2011 le. Programme d'Intégration des Forêts Sacrées dans le. Système des Aires Protégées (PIFSAP) en vu de,.

  2. Conventional and Indigenous Biodiversity Conservation Approach: A Comparative Study of Jachie Sacred Grove and Nkrabea Forest Reserve

    Directory of Open Access Journals (Sweden)

    Samuel Boadi

    2017-01-01

    Full Text Available Conventional managed forests and sacred groves are seldom assessed to determine their effectiveness in biodiversity conservation strategies. This study investigated tree and insect diversity in Jachie sacred grove (JSG and Nkrabea forest reserve (NFR in Ashanti region, Ghana. The study area constituted eight plots of 50 × 50 m along two 300 m long transects. Insects were sampled in eight pitfall traps, diagonally between the transects. Out of 150 individuals, 13 species in NFR and 15 species from JSG were registered. Celtis mildbraedii was the most dominant species in NFR = 43.18% and JSG = 23.58%. Mean DBH showed a significant relationship with basal area in NFR and JSG. Tree diversity and richness were higher in JSG (H′ = 1.43–2.3 ± 0.10; D = 1.8–3.69 ± 0.30 compared to NFR (H′ = 0.86–1.56 ± 0.09; D = 1.1–2.3 ± 0.57. However, insect diversity was higher in NFR (H′ = 1.34 ± 0.10 than in JSG (H′ = 0.5 ± 0.005. Camponotus furvus and Pachycondyla tarsata were most abundant in JSG and NFR, respectively. These findings will help conservationists work closely with traditional authorities in protecting sacred groves as key biodiversity hotspots.

  3. Places of Faith: A Reflection on Landscape of Manila Cathedral Plaza de Roma and Istiqlal Mosque Sacred Grounds of Jakarta

    Science.gov (United States)

    Pujalte, MM; Navarra, N.

    2017-10-01

    Crossing boundaries of faith from Manila to Jakarta, this study is to classify the open spaces in their sacred grounds according to its characteristics, elements, use of space and hierarchy of importance in landscape design approach. The reflection of their religious landscape in preserving the traditional, and exploring the non-traditional aspect of their landscape design in global setting is carried out thru a spatial analysis for Plaza de Roma of Manila Cathedral and the sacred grounds of Istiqlal Mosque. The design framework would tackle: concepts, planning approach, functional symbolic values, and aesthetics used. The data and information are all examined based on observation, historical background, analyses, and literature content in determining spatial functions. Finally, when results are completed, this will give a better understanding on the importance of open areas in Manila and Jakarta’s sacred spaces; paving way for a better sense of comfort in spiritual contemplation. This will also help reveal the commonalities in spiritual practices between Islam and Christianity, and the role of landscape in their religion and faith.

  4. Changes in pyridine metabolism profile during growth of trigonelline-forming Lotus japonicus cell cultures.

    Science.gov (United States)

    Yin, Yuling; Matsui, Ayu; Sakuta, Masaaki; Ashihara, Hiroshi

    2008-12-01

    Changes in the profile of pyridine metabolism during growth of cells were investigated using trigonelline-forming suspension-cultured cells of Lotus japonicus. Activity of the de novo and salvage pathways of NAD biosynthesis was estimated from the in situ metabolism of [(3)H] quinolinic acid and [(14)C] nicotinamide. Maximum activity of the de novo pathway for NAD synthesis was found in the exponential growth phase, whereas activity of the salvage pathway was increased in the lag phase of cell growth. Expression profiles of some genes related to pyridine metabolism were examined using the expression sequence tags obtained from the L. japonicus database. Transcript levels of NaPRT and NIC, encoding salvage enzymes, were enhanced in the lag phase of cell growth, whereas the maximum expression of NADS was found in the exponential growth phase. Correspondingly, the activities of the salvage enzymes, nicotinate phosphoribosyltransferase (EC 2.4.2.11) and nicotinamidase (EC 3.5.1.19), increased one day after transfer of the stationary phase cells to the fresh medium. The greatest in situ trigonelline synthesis, both from [(3)H] quinolinic acid and [(14)C] nicotinamide, was found in the stationary phase of cell growth. The role of trigonelline in leguminous plants is discussed.

  5. A Rare Class of New Dimeric Naphtoquiones from Diospyros lotus have Multidrug Reversal and Antiproliferative Effects

    Directory of Open Access Journals (Sweden)

    Dr. Abdur eRauf

    2015-12-01

    Full Text Available Three new dimeric naphthoquinones, 5,4′-dihydroxy-1′-methoxy-6,6′-dimethyl-7,3′-binaphthyl-1,4,5′,8′-tetraone (1, 5′,8′-dihydroxy-5-methoxy-6,6′-dimethyl-7,3′-binaphthyl-1,4,1′,4′-tetraone (2 and 8,5′,8′-trihydroxy-6,6′-dimethyl-7,3′-binaphthyl-1,4,1′,4′-tetraone (3, were isolated from the roots of Diospyros lotus. Their structures were elucidated by spectroscopic techniques, including 1D and 2D NMR, such as HSQC, HMBS, NOESY and J resolved. Compounds 1-3 were evaluated for their effects on the reversion of multidrug resistance (MDR mediated by P-glycoprotein through use of the rhodamine-123 exclusion screening test on human ABCB1 gene transfected L5178Y mouse T-cell lymphoma. Compounds 1-3 were also assessed for their antiproliferative and cytotoxic effects on L5178 and L5178Y mouse T-cell lymphoma lines. Both 1 and 2 exhibited promising antiproliferative and MDR-reversing effects in a dose dependent manner. The effects of the tested compounds on the activity of doxorubicin were observed to vary from slight antagonism to antagonism.

  6. Procyanidins extracted from the lotus seedpod ameliorate scopolamine-induced memory impairment in mice.

    Science.gov (United States)

    Xu, Jiqu; Rong, Shuang; Xie, Bijun; Sun, Zhida; Zhang, Li; Wu, Hailei; Yao, Ping; Zhang, Yunjian; Liu, Liegang

    2009-12-01

    The major purpose of this study was to determine the effect of procyanidins extracted from the lotus seedpod (LSPC) on the learning and memory impairments induced by scopolamine (1 mg/kg, i.p.) in mice. The capacities of memory and learning were evaluated by the Morris water maze and the step-down avoidance test. LSPC (50, 100, 150 mg/kg BW, p.o.) significantly reversed scopolamine-induced learning and memory impairments in the Morris water maze test, as evaluated by shortened escape latency and swimming distance. In the step-down avoidance test, LSPC (50, 100, 150 mg/kg BW, p.o.) treatment significantly reduced the number of errors and shortened latency compared with that of scopolamine. In addition, LSPC was also found to inhibit acetyl cholinesterase (AChE) activity. These results of this study suggest that LSPC may play a useful role in the treatment of cognitive impairment caused by AD and aging. Copyright (c) 2009 John Wiley & Sons, Ltd.

  7. Structural and physicochemical properties of lotus seed starch treated with ultra-high pressure.

    Science.gov (United States)

    Guo, Zebin; Zeng, Shaoxiao; Lu, Xu; Zhou, Meiling; Zheng, Mingjing; Zheng, Baodong

    2015-11-01

    Aqueous lotus seed starch suspensions (15%, w/w) were subjected to ultra-high pressure treatment (UHP, 100-600 MPa) for 30 min. The effects of UHP treatment on the structural and physicochemical properties of starch were investigated. The SEM and laser diffraction particle size analysis revealed that UHP treatment affected the shape and size distribution of starch granules. The morphological structure of starch was completely destroyed at 600 MPa, indicating complete gelatinization. Analysis of HPSEC-MALLS-RI suggested that the dispersity index of UHP-treated starch were decreased from 1.28 to 1.11. According to XRD analyses, UHP treatment converted native starch (C-type) into a B-type pattern. The swelling power and solubility presented a significant decrease at 85 and 95 °C, but opposite trends were found at 55-75 °C. The DSC results indicated a reduction in gelatinization temperatures and enthalpy with increasing pressure treatment. The RVA viscograms revealed that UHP-treated starch showed a decreased breakdown and setback viscosity, reflecting lower retrogradation tendency compared to native starch. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Phytochemical and biological study of the aerial parts of Lotus Lalambensis growing in Saudi Arabia

    International Nuclear Information System (INIS)

    El-Youssef, Hanan M.; Murphy, Brian T.; Amer, Masouda E.; Abdel-Kader, Maged S.; Kingston, David J.I.

    2008-01-01

    Phytochemical investigation of the aerial parts of Lotus lalambensis Schwenif resulted in the isolation and identification of 20 known compounds. Liquid-Liquid fractionation of the crude extract followed by chromatographic purification resulted in the isolation of lupeol, b-sitosterol, oleanolic acid, b-sitosterol glucoside and stigmasterol glucoside from petroleum ether fraction. The chloroform fraction afforded heptadecanol, kaempferol (1), kaempferol-3-O-a-L-rhamnoside (2), lotaustralin (3) epilotaaustralin (4), linamarin (5), kaempferol-3, 7-di-O-a-L-rhamnopyranoside (kaempferitin) (6) and ethyl-O-b-glucopyranoside (7). From the ethyl acetate fraction three simple rhamnosyl derivatives; butyl-O-a-L-rhamnopyranoside (8) methyl-O-a-L-rhamnopyranoside (9) and methyl-O-b-rhamnopyranoside (10) were obtained. Kaempferol-3-O-b-D-glucopyranoside-7-O-a-L-rhamnopyranoside (11), kaempferol-3-O-a- [b-D-glucopyranosyl-(1''''-2'''')-L- rhamnopyranoside]-7-O-a-L- rhamnopyranoside (12), kaempferol-3-O-b-D- rhamnopyranoside-7-O-a-[b-D-glucopyranosyl -(1'''-2'')-L- rhamnopyranoside] (13) and the myo-inositol (+) D-pinitol (14) were isolated from the butanol extract. The total extract and the different fractions were evaluated for their possible estrogenic, anti-inflammatory and anti-platelets aggregation activities. The chloroform extract showed the highest estrogenic activity, while the petroleum ether was the best in protection against inflammation induced by carrageenan. The strongest inhibition of platelet aggregations were observed with the aqueous fraction. (author)

  9. Comparative phenological study of ziziphus lotus (l.) lam. in the arid area of tunisia

    International Nuclear Information System (INIS)

    Zouaoui, R.; Ksontini, M.

    2014-01-01

    Ziziphus lotus (L.) Lam. is spontaneous plant (Rhamnaceae). It is of great ecological value and versatile, but it is endangered. To show the variability of phenological phases of this taxon, a study of phenology was conducted in arid Tunisia: ((Bou Hedma (BH); Matmata (MM); Medenine (Amra) (MD) and Oued Zes (OZ)) during two years (2010 and 2011). Our results show that the different phases, especially the flowering and fruiting coincide with the dry season. The observation of these shrubs in different phenological states shows a high variation between provenances (intersite) and between individual (intrasite). In 2011, the leafing out from BH presents a precocity and prolongation in time compared to others. The flowering is enough spread over time and present with fruiting phase at a given time. In 2010, the provenances of MD and MM are more precocious compared to other. By against, in 2011, all the provenances bloom at the same time. In BH, the fructification is the most precocious compared to others. These variations between provenances can be explained by the influence of temperature, soil moisture, photoperiod, exposure and soil texture. (author)

  10. Effects of Zizyphus lotus L. (Desf.) polyphenols on Jurkat cell signaling and proliferation.

    Science.gov (United States)

    Abdoul-Azize, Souleymane; Bendahmane, Malika; Hichami, Aziz; Dramane, Gado; Simonin, Anne-Marie; Benammar, Chahid; Sadou, Hassimi; Akpona, Simon; El Boustani, Es-Saddik; Khan, Naim A

    2013-02-01

    We assessed the effects of Zizyphus lotus L. (Desf.) polyphenols (ZLP) on T-cell signaling and proliferation. Our results showed that ZLP exerted no effect on the increases in intracellular free calcium concentrations, [Ca(2+)]i, in human Jurkat T-cells. However, ZLP modulated the thapsigargin-induced increases in [Ca(2+)]i in these cells. ZLP treatment was found to decrease the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). In addition, ZLP induced a rapid (t1/2=33s) and dose-dependent decrease in intracellular pH (pHi) in human Jurkat T-cells. Furthermore, ZLP significantly curtailed T-cell proliferation by diminishing their progression from S to G2/M phase of cell cycle, and the expression of interleukin-2 (IL-2) mRNA. Taken together, the results of the present study demonstrate that ZLP modulate cell signaling and exert immunosuppressive effects in human T-cells. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. Preparing superhydrophobic copper surfaces with rose petal or lotus leaf property using a simple etching approach

    Science.gov (United States)

    Talesh Bahrami, H. R.; Ahmadi, B.; Saffari, H.

    2017-05-01

    A facile chemical etching process is developed to fabricate superhydrophobic copper surfaces. In the first step, cleaned copper surfaces immersed in ferric chloride (FeCl3) solutions with specific concentrations for different times. Etched surfaces exhibit the maximum contact angle of 140°. They have large sliding angle and water droplets stuck to the surface even if they were turned upside down which is well-known as rose petal effect. After stearic acid modification of etched surfaces, their contact angle slightly increased to above 150° and sliding angle decreased to smaller than 10° in some cases, which is same as lotus plant leaves property against water. Inspecting SEM images of etched surfaces reveals that many micro-nano structures forming blossom like buildings with curved petals of nanoscale thicknesses are formed. The micro-nano structures sizes and shapes affecting surface hydrophobicity are regulated by controlling reaction times and etchant solution concentrations. X-ray diffraction (XRD) analysis is done on a sample before and after of the etching process where patterns indicate that the same compositions present on the sample.

  12. Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

    Directory of Open Access Journals (Sweden)

    Camille E Granada

    2014-06-01

    Full Text Available This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.

  13. Beyond the lotus effect: roughness influences on wetting over a wide surface-energy range.

    Science.gov (United States)

    Spori, Doris M; Drobek, Tanja; Zürcher, Stefan; Ochsner, Mirjam; Sprecher, Christoph; Mühlebach, Andreas; Spencer, Nicholas D

    2008-05-20

    To enhance our understanding of liquids in contact with rough surfaces, a systematic study has been carried out in which water contact angle measurements were performed on a wide variety of rough surfaces with precisely controlled surface chemistry. Surface morphologies consisted of sandblasted glass slides as well as replicas of acid-etched, sandblasted titanium, lotus leaves, and photolithographically manufactured golf-tee shaped micropillars (GTMs). The GTMs display an extraordinarily stable, Cassie-type hydrophobicity, even in the presence of hydrophilic surface chemistry. Due to pinning effects, contact angles on hydrophilic rough surfaces are shifted to more hydrophobic values, unless roughness or surface energy are such that capillary forces become significant, leading to complete wetting. The observed hydrophobicity is thus not consistent with the well-known Wenzel equation. We have shown that the pinning strength of a surface is independent of the surface chemistry, provided that neither capillary forces nor air enclosure are involved. In addition, pinning strength can be described by the axis intercept of the cosine-cosine plot of contact angles for rough versus flat surfaces with the same surface chemistries.

  14. Two Lotus japonicus symbiosis mutants impaired at distinct steps of arbuscule development.

    Science.gov (United States)

    Groth, Martin; Kosuta, Sonja; Gutjahr, Caroline; Haage, Kristina; Hardel, Simone Liesel; Schaub, Miriam; Brachmann, Andreas; Sato, Shusei; Tabata, Satoshi; Findlay, Kim; Wang, Trevor L; Parniske, Martin

    2013-07-01

    Arbuscular mycorrhiza (AM) fungi form nutrient-acquiring symbioses with the majority of higher plants. Nutrient exchange occurs via arbuscules, highly branched hyphal structures that are formed within root cortical cells. With a view to identifying host genes involved in AM development, we isolated Lotus japonicus AM-defective mutants via a microscopic screen of an ethyl methanesulfonate-mutagenized population. A standardized mapping procedure was developed that facilitated positioning of the defective loci on the genetic map of L. japonicus, and, in five cases, allowed identification of mutants of known symbiotic genes. Two additional mutants representing independent loci did not form mature arbuscules during symbiosis with two divergent AM fungal species, but exhibited signs of premature arbuscule arrest or senescence. Marker gene expression patterns indicated that the two mutants are affected in distinct steps of arbuscule development. Both mutants formed wild-type-like root nodules upon inoculation with Mesorhizobium loti, indicating that the mutated loci are essential during AM but not during root nodule symbiosis. © 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.

  15. Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

    Science.gov (United States)

    Granada, Camille E.; Strochein, Marcos; Vargas, Luciano K.; Bruxel, Manuela; de Sá, Enilson Luiz Saccol; Passaglia, Luciane M.P.

    2014-01-01

    This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species. PMID:25071405

  16. Fatigue crack initiation and propagation in lotus-type porous material

    Directory of Open Access Journals (Sweden)

    S. Glodež

    2016-01-01

    Full Text Available The investigation of fatigue strength of lotus-type structure with nodular cast iron as a base material using computational model is analysed in present study. The irregular pores distribution in transversal and longitudinal direction, regarding the external loading, is considered in the computational models. The complete fatigue process of analyzed porous structure is then divided into the crack initiation (Ni and crack propagation (Np period where the total fatigue life (N is defined as: N = Ni + Np. The crack initiation period is determined using strain life approach where elastic-plastic numerical analysis is performed to obtain the total strain amplitude in the critical stress fields around the pores. The simplified universal slope method is then used to determine the number of stress cycles, Ni, required for formation of initial cracks. The number of stress cycles, Np, required for crack propagation from initial to the critical crack length is also numerically determined using finite element (FE models, in the frame of Abaqus computation FEM code. The maximum tensile stress (MTS criterion is considered when analyzing the crack path inside the porous structure. The performed computational analyses show that stress concentrations around individual pores are higher when external loading is acting in transversal direction in respect to the pore distribution. Therefore, further computational analyses regarding crack initiation and crack propagation period have been done only for pores distribution in transversal direction.

  17. Allomorph distribution and granule structure of lotus rhizome C-type starch during gelatinization.

    Science.gov (United States)

    Cai, Canhui; Cai, Jinwen; Man, Jianmin; Yang, Yang; Wang, Zhifeng; Wei, Cunxu

    2014-01-01

    The allomorph distribution and granule structure of C-type starch from lotus rhizomes were investigated using a combination of techniques during gelatinization. The disruption of crystallinity during gelatinization began from the end distant from the eccentric hilum and then propagated into the center of granule. The periphery of hilum end was finally gelatinized, accompanied by high swelling. The crystallinity changed from C-type to A-type via CA-type during gelatinization, and finally became amorphous structure. The amylose content, crystal degree, helix content, ratio of 1045/1022cm(-1), and peak intensity of crystalline lamellae of gelatinizing starch significantly decreased after 70°C. The amorphous content and ratio of 1022/995cm(-1) increased after 70°C. This study elucidated that B-type allomorph was mainly arranged in the distal region of eccentric hilum, A-type allomorph was mainly located in the periphery of hilum end, and the center of granule was a mixed distribution of A- and B-type allomorphs. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Shanwei [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); Zhou, Libin, E-mail: libinzhou@impcas.ac.cn [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); Li, Wenjian; Du, Yan [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); Yu, Lixia; Feng, Hui; Mu, Jinhu [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); Chen, Yuze [College of Life Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou, Gansu Province 730070 (China)

    2016-09-15

    Carbon ion beam irradiation is a powerful method for creating mutants and has been used in crop breeding more and more. To investigate the effects of carbon ion beams on Lotus japonicus, dry seeds were irradiated by 80 MeV/u carbon ion beam at dosages of 0, 100, 200, 300, 400, 500 and 600 Gy. The germination rate, survival rate and root length of M{sub 1} populations were explored and the dose of 400 Gy was selected as the median lethal dose (LD{sub 50}) for a large-scale mutant screening. Among 2472 M{sub 2} plants, 127 morphological mutants including leaf, stem, flower and fruit phenotypic variation were found, and the mutation frequency was approximately 5.14%. Inter simple sequence repeat (ISSR) assays were utilized to investigate the DNA polymorphism between seven mutants and eight plants without phenotypic variation from M{sub 2} populations. No remarkable differences were detected between these two groups, and the total polymorphic rate was 0.567%.

  19. Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase

    Directory of Open Access Journals (Sweden)

    Margarita eGarcía-Calderón

    2015-09-01

    Full Text Available This paper was aimed to investigate the possible implications of the lack of plastidic glutamine synthetase (GS2 in phenolic metabolism during stress responses in the model legume Lotus japonicus. Important changes in the transcriptome were detected in a GS2 mutant called Ljgln2-2, compared to the wild type, in response to two separate stress conditions, such as drought or the result of the impairment of the photorespiratory cycle. Detailed transcriptomic analysis showed that the biosynthesis of phenolic compounds was affected in the mutant plants in these two different types of stress situations. For this reason, the genes and metabolites related to this metabolic route were further investigated using a combined approach of gene expression analysis and metabolite profiling. A high induction of the expression of several genes for the biosynthesis of different branches of the phenolic biosynthetic pathway was detected by qRT-PCR. The extent of induction was always higher in Ljgln2-2, probably reflecting the higher stress levels present in this genotype. This was paralleled by accumulation of several kaempferol and quercetine glycosides, some of them described for the first time in L. japonicus, and of high levels of the isoflavonoid vestitol. The results obtained indicate that the absence of GS2 affects different aspects of phenolic metabolism in L .japonicus plants in response to stress.

  20. Simultaneous qualitative and quantitative analysis of flavonoids and alkaloids from the leaves of Nelumbo nucifera Gaertn. using high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry.

    Science.gov (United States)

    Guo, Yujie; Chen, Xi; Qi, Jin; Yu, Boyang

    2016-07-01

    A reliable method, combining qualitative analysis by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and quantitative assessment by high-performance liquid chromatography with photodiode array detection, has been developed to simultaneously analyze flavonoids and alkaloids in lotus leaf extracts. In the qualitative analysis, a total of 30 compounds, including 12 flavonoids, 16 alkaloids, and two proanthocyanidins, were identified. The fragmentation behaviors of four types of flavone glycoside and three types of alkaloid are summarized. The mass spectra of four representative components, quercetin 3-O-glucuronide, norcoclaurine, nuciferine, and neferine, are shown to illustrate their fragmentation pathways. Five pairs of isomers were detected and three of them were distinguished by comparing the elution order with reference substances and the mass spectrometry data with reported data. In the quantitative analysis, 30 lotus leaf samples from different regions were analyzed to investigate the proportion of eight representative compounds. Quercetin 3-O-glucuronide was found to be the predominant constituent of lotus leaf extracts. For further discrimination among the samples, hierarchical cluster analysis, and principal component analysis, based on the areas of the eight quantitative peaks, were carried out. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Geomorphology of Dra Abu el-Naga (Egypt): The basis of the funerary sacred landscape

    Science.gov (United States)

    Bardají, T.; Martínez-Graña, A.; Sánchez-Moral, S.; Pethen, H.; García-González, D.; Cuezva, S.; Cañaveras, J. C.; Jiménez-Higueras, A.

    2017-07-01

    A geological and geomorphological analysis has been performed in the necropolis of Dra Abu el-Naga in order to understand the role played by these two factors in the development of the sacred landscape. The investigation focuses upon two aspects of the development of the necropolis, the selection criteria for tomb location and the reconstruction of the ancient funerary landscape. Around 50 tombs were surveyed, analysing the characteristics of their host rock and classifying them according to a modified Rock Mass Rating Index, in order to understand how rock quality affected tomb construction. This analysis resulted in the definition of five rock-quality classes (I to V) from very good to very poor rock. The geological study also resulted in a proposed geological-geomorphological model for the evolution of this zone of the Theban necropolis that complements previous works by other authors. Due to the lack of precise dating evidence this chronology is a relative one and is based on the chronology given by other authors for similar deposits and events. Two catastrophic events, represented by mega-landslides, have been identified, the first one predates the deposition of early Pleistocene fluvial deposits, and the second one possibly occurred during the middle-late Pleistocene. Two weathering surfaces developed under wetter than present climatic conditions and have been tentatively correlated to the mid-late Pleistocene humid period and the African-Humid Period (early-mid Holocene).

  2. Palliative Nursing and Sacred Medicine: A Holistic Stance on Entheogens, Healing, and Spiritual Care.

    Science.gov (United States)

    Rosa, William E; Hope, Stephanie; Matzo, Marianne

    2018-04-01

    The fields of palliative and holistic nursing both maintain a commitment to the care of the whole person, including a focus on spiritual care. Advanced serious illness may pose a plethora of challenges to patients seeking to create meaning and purpose in their lives. The purpose of this article is to introduce scholarly dialogue on the integration of entheogens, medicines that engender an experience of the sacred, into the spiritual and holistic care of patients experiencing advanced serious illness. A brief history of the global use of entheogens as well as a case study are provided. Clinical trials show impressive preliminary findings regarding the healing potential of these medicinal agents. While other professions, such as psychology, pharmacy, and medicine, are disseminating data related to patient outcomes secondary to entheogen administration, the nursing literature has not been involved in raising awareness of such advancements. Research is illustrating their effectiveness in achieving integrative experiences for patients confronting advanced serious illness and their ability to promote presence, introspection, decreased fear, and increased joy and acceptance. Evidence-based knowledge surrounding this potentially sensitive topic is necessary to invite understanding, promote scientific knowledge development, and create healing environments for patients, nurses, and researchers alike.

  3. Music As a Sacred Cue? Effects of Religious Music on Moral Behavior.

    Science.gov (United States)

    Lang, Martin; Mitkidis, Panagiotis; Kundt, Radek; Nichols, Aaron; Krajčíková, Lenka; Xygalatas, Dimitris

    2016-01-01

    Religion can have an important influence in moral decision-making, and religious reminders may deter people from unethical behavior. Previous research indicated that religious contexts may increase prosocial behavior and reduce cheating. However, the perceptual-behavioral link between religious contexts and decision-making lacks thorough scientific understanding. This study adds to the current literature by testing the effects of purely audial religious symbols (instrumental music) on moral behavior across three different sites: Mauritius, the Czech Republic, and the USA. Participants were exposed to one of three kinds of auditory stimuli (religious, secular, or white noise), and subsequently were given a chance to dishonestly report on solved mathematical equations in order to increase their monetary reward. The results showed cross-cultural differences in the effects of religious music on moral behavior, as well as a significant interaction between condition and religiosity across all sites, suggesting that religious participants were more influenced by the auditory religious stimuli than non-religious participants. We propose that religious music can function as a subtle cue associated with moral standards via cultural socialization and ritual participation. Such associative learning can charge music with specific meanings and create sacred cues that influence normative behavior. Our findings provide preliminary support for this view, which we hope further research will investigate more closely.

  4. St. Patriarch Tikhon and Confi scation of Sacred Objects from Churches in 1922

    Directory of Open Access Journals (Sweden)

    Ivanov Sergei, deacon

    2017-06-01

    Full Text Available This paper aims to clarify the reliability of the canonical foundation of St. Patriarch Tikhon’s position for the protection of Church relics taken from churches in 1922 under the slogan of famine relief. Conclusions as to the legitimacy of the Patriarch’s point of view are based on the reconstruction of the historical context of the confi scation of Church values, as well as on a more detailed consideration of the implicit aspects of the controversy between the opponents on this issue. Patriarch’s appeal to the 73rd Apostolic Rule and the 10th rule of the “Prime-Second” Local Council of Constantinople resulted from the three significant reasons: first, the inability to publicly appeal to decisions of the Local Council of the Russian Orthodox Church of 1917–1918; second, the distrust of the Church community of the government’s slogans; third, the beginning of confiscation of Church values by the state authorities that was uncoordinated with Head of the Church. St. Tikhon managed to adequately protect the ecclesiastical point of view both in the Revolutionary Tribunal and during the interrogations after his arrest. Fulfilling the requirements of the Antireligious Commission of the Central Committee of RKP(b, necessary for his release from prison in 1923, Patriarch did not change his views on the confiscation of sacred objects, and it was he who had the last word in this controversy.

  5. Music As a Sacred Cue? Effects of Religious Music on Moral Behavior

    Science.gov (United States)

    Lang, Martin; Mitkidis, Panagiotis; Kundt, Radek; Nichols, Aaron; Krajčíková, Lenka; Xygalatas, Dimitris

    2016-01-01

    Religion can have an important influence in moral decision-making, and religious reminders may deter people from unethical behavior. Previous research indicated that religious contexts may increase prosocial behavior and reduce cheating. However, the perceptual-behavioral link between religious contexts and decision-making lacks thorough scientific understanding. This study adds to the current literature by testing the effects of purely audial religious symbols (instrumental music) on moral behavior across three different sites: Mauritius, the Czech Republic, and the USA. Participants were exposed to one of three kinds of auditory stimuli (religious, secular, or white noise), and subsequently were given a chance to dishonestly report on solved mathematical equations in order to increase their monetary reward. The results showed cross-cultural differences in the effects of religious music on moral behavior, as well as a significant interaction between condition and religiosity across all sites, suggesting that religious participants were more influenced by the auditory religious stimuli than non-religious participants. We propose that religious music can function as a subtle cue associated with moral standards via cultural socialization and ritual participation. Such associative learning can charge music with specific meanings and create sacred cues that influence normative behavior. Our findings provide preliminary support for this view, which we hope further research will investigate more closely. PMID:27375515

  6. Maria Giulia Dondero, Le sacré dans l’image photographique.

    Directory of Open Access Journals (Sweden)

    Jean Fisette

    2010-01-01

    Full Text Available

     

    Maria Giulia Dondero, Le sacré dans l’image photographique. Études sémiotiques, Paris, 2009, Lavoisier, («Formes et sciences», 234p.

    ISBN-13:

     

     

    978-2746224537

  7. POETICS OF TRANSCENDENCE: STYLISTIC REDUCTION AS A TOOL FOR REPRESENTATION OF SACRED MEANINGS

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    Elena Brazgovskaya

    2016-10-01

    Full Text Available The main direction of the work is connected to the representation of abstract (transcendent objects in music and literature. The article analyses "Cantus in Memoriam Benjamin Britten" by Arvo Pärt and some poems of Czesław Miłosz. The metaphysical dimension of reality involves forms and things, existing beyond the boundaries of empirical perception and, at first sight, beyond the descriptive practices. Abstract objects are available in intellectual experience, but culture must transform them into a symbolic form. As a rule, it is connected to the practice of art minimalism. The essence of minimalism is the reduction of number of stylistic tools and “purification” the perception from the visual / auditory images (not a mimetic use of language. For the representation of the sacred Pärt uses only mensural canon form, scale and chord. These “characters” are deprived of descriptive function, but have symbolic potential (canon as a sign of stopped time, the eternal return. The distinctive feature of the Miłoszʼs style is the pursuit to “clean” the signs (indexical and symbolic. There is the reverse side of language distillation: the rejection of the subjective position, emotional experience, the distance between the person and the object of representation.

  8. The Park 51/Ground Zero Controversy and Sacred Sites as Contested Space

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    Jeanne Halgren Kilde

    2011-07-01

    Full Text Available The Park 51 controversy swept like wildfire through the media in late August of 2010, fueled by Islamophobes who oppose all advance of Islam in America. Yet the controversy also resonated with many who were clearly not caught up in the fear of Islam. This article attempts to understand the broader concern that the Park 51 project would somehow violate the Ground Zero site, and, thus, as a sign of "respect" should be moved to a different location, an argument that was invariably articulated in “spatial language” as groups debated the physical and spatial presence of the buildings in question, their relative proximity, and even the shadows they cast. This article focuses on three sets of spatial meanings that undergirded these arguments: the site as sacred ground created through trauma, rebuilding as retaliation for the attack, and the assertion of American civil religion. The article locates these meanings within a broader civic discussion of liberty and concludes that the spatialization of the controversy opened up discursive space for repressive, anti-democratic views to sway even those who believe in religious liberty, thus evidencing a deep ambivalence regarding the legitimate civic membership of Muslim Americans.

  9. Politics, religion and space. About places of mediation and sacred space among the Gurage of Ethiopia

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    PALMISANO, Antonio Luigi

    2016-12-01

    Full Text Available The main faiths among the Gurage of Ethiopia – Orthodox Christianity, Islam and autochthonous polytheism – provide a constellation of integrative principles for territorial and descent segmentation. Religious leaders and ritual specialists of the three confessions have always represented the unity of Gurage tribes facing the outside world and still receive tributes of cattle, sheep, goats, honey, ensete, money etc. The ethnic court of justice of the Gurage, the yejoka, has re-dimensioned its office, in order to prevent the rise of central instances within the process of political mediation between juxtaposed lineages and territorial segments. The article focuses on the identification and institutionalisation of sacred places among these tribes, the places where the personae, the lineages and residential groups interact within the self-representation processes of Gurage society. The theological and liturgical pluralism, practised by the three faiths, confirms the Gurage’s capacity to answer to the many and contradictory stimuli of modern society, of market economy, of political, administrative and territorial transformation which takes place in the world. This religious plurality in a single family, in a single person, unveils religion as an answer, or rather as a structure of satisfactory answers.

  10. Sinan and Palladio: A comparative morphological analysis of two sacred precedents

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    Buthayna Eilouti

    2017-06-01

    Full Text Available An exploratory study on the design schemes underlying two architectural precedents is presented and discussed. The study compares the formalistic compositions of two prominent precedents on multiple levels. These levels include the morphological, semantic, and pragmatic layers. The morphological aspects of these layers are emphasized. The study aims to identify the commonalities and differences between two cases of classical sacred architecture in terms of their morphometric and morphosyntactic structures. These cases are represented by Palladio׳s II Redentore Church and Sinan׳s Süleymaniye Mosque designs. The scope of the analysis focuses on the syntax and mathematics of the floor plans, and the facade and section of each case are referenced occasionally. Within this scope, the proportional system that regulates the compositions and eidonomy of the precedents are scrutinized. Findings resulting from the comparative analysis are reported and then discussed. The study shows that the two ostensibly different precedents share obscurely many commonalities, which are greater than what their initial appearances suggest.

  11. Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

    Science.gov (United States)

    Zgadzaj, Rafal; Garrido-Oter, Ruben; Jensen, Dorthe Bodker; Koprivova, Anna; Schulze-Lefert, Paul; Radutoiu, Simona

    2016-01-01

    Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive nodule- and root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance. PMID:27864511

  12. EFFETS ANTIOXYDANTS ET IMMUNOMODULATEURS D’UNE PLANTEMEDICINALE NORD AFRICAINE, ZIZYPHUS LOTUS L. (SEDRA) : ETUDE DES DIFFERENTS EXTRAITS

    OpenAIRE

    BENAMMAR, Chahid Elhocine

    2011-01-01

    Cette étude porte sur la valorisation d’un arbrisseau fruitier appelé Zizyphus lotus L. (Rhamnacées) connu dans la région de Tlemcen sous le nom vernaculaire Sedra. C’est une plante utile, en médecine populaire, pour soigner le tube digestif, le foie et les affections respiratoires. Elle est connue pour ses activités : anti-inflammatoire, anti-ulcérogénique, analgésique et antidiabétique. Nous avons démontré dans un premier temps que les différentes parties de cette plante poss...

  13. Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules.

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    Anna Zdyb

    Full Text Available Jasmonic acid (JA, its derivatives and its precursor cis-12-oxo phytodienoic acid (OPDA form a group of phytohormones, the jasmonates, representing signal molecules involved in plant stress responses, in the defense against pathogens as well as in development. Elevated levels of JA have been shown to play a role in arbuscular mycorrhiza and in the induction of nitrogen-fixing root nodules. In this study, the gene families of two committed enzymes of the JA biosynthetic pathway, allene oxide synthase (AOS and allene oxide cyclase (AOC, were characterized in the determinate nodule-forming model legume Lotus japonicus JA levels were to be analysed in the course of nodulation. Since in all L. japonicus organs examined, JA levels increased upon mechanical disturbance and wounding, an aeroponic culture system was established to allow for a quick harvest, followed by the analysis of JA levels in whole root and shoot systems. Nodulated plants were compared with non-nodulated plants grown on nitrate or ammonium as N source, respectively, over a five week-period. JA levels turned out to be more or less stable independently of the growth conditions. However, L. japonicus nodules formed on aeroponically grown plants often showed patches of cells with reduced bacteroid density, presumably a stress symptom. Immunolocalization using a heterologous antibody showed that the vascular systems of these nodules also seemed to contain less AOC protein than those of nodules of plants grown in perlite/vermiculite. Hence, aeroponically grown L. japonicus plants are likely to be habituated to stress which could have affected JA levels.

  14. A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus.

    Science.gov (United States)

    Kimura, Mitsuhiro; Cutler, Sean; Isobe, Sachiko

    2015-01-01

    Agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. However, the limitations of Agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants difficult. Transformation efficiency in many legume species, including soybean and the common bean, has been reported to be quite low. To improve the transformation procedure in legumes, we screened for chemicals that increase the transformation efficiency of Lotus japonicus, a model legume species. A Chemical library was screened and chemicals that increase in transient transformation efficiency of L. japonicus accession, Miyakojima MG-20 were identified. The transient transformation efficiency was quantified by reporter activity in which an intron-containing reporter gene produces the GUS protein only when the T-DNA is expressed in the plant nuclei. We identified a phenolic compound, chloroxynil, which increased the genetic transformation of L. japonicus by Agrobacterium tumefaciens strain EHA105. Characterization of the mode of chloroxynil action indicated that it enhanced Agrobacterium-mediated transformation through the activation of the Agrobacterium vir gene expression, similar to acetosyringone, a phenolic compound known to improve Agrobacterium-mediated transformation efficiency. Transient transformation efficiency of L. japonicus with 5 μM chloroxynil was 60- and 6- fold higher than that of the control and acetosyringone treatment, respectively. In addition, transgenic L. japonicus lines were successfully generated by 5 μM chloroxynil treatment.Furthermore, we show that chloroxynil improves L. japonicus transformation by Agrobacterium strain GV3101 and rice transformation. Our results demonstrate that chloroxynil significantly improves Agrobacterium tumefaciens-mediated transformation efficiency of various agriculturally important crops.

  15. Secondary Fitometabolitos that affect the nutritional value of Lotus corniculatus as forage for ruminant animals

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    Luz Elena Santacoloma Varón

    2014-05-01

    Full Text Available In the highlands of Bogota at 2600 masl, temperature 14°C and 1013 mm of precipitation, three plots have been furbished by modifying the physico- chemical conditions of the soil and planted the species Lotus corniculatus to evaluate content of condensed tannins, hydrolysable tannins, phenols, tannins that precipitate protein, saponins and alkaloids. The purpose was to determine the effect of soil conditions on the concentration of secondary phytobiomolecules. Analytical and instrumental techniques of AOAC were applied to determine the presence of secondary phytometabolites and the obtained results were put through a simple variance analysis. Additionally a multiple correlation analysis was done, using the Pearson’s coefficient. It was found that the production of condensed tannins, tannins and hydrolysable tannins that precipitate protein in L. corniculatus, varies, with highly different significant statistics according to the soil type. The content of total phenols, in the wrapping did not show significative differences among treatments (P>0.05. The ammoniacal nitrogen of the soil correlated negatively and significantly with the concentration of hydrolysable tannins and with the tannins that precipitate protein. It was found negative correlation between the effective cationic exchange capacity of soils and production of condensed tannins and total phenols in the studied plant. A correlation between the change in pH in the soil and the content of condensed tannins and and hihgly significant with the tannins that precipitate protein was identified. It is concluded that the variation of the physico-chemical conditions of the soil affect the production of secondary metabolites in L. corniculatus.

  16. Heterogeneity in the expression and subcellular localization of POLYOL/MONOSACCHARIDE TRANSPORTER genes in Lotus japonicus.

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    Lu Tian

    Full Text Available Polyols can serve as a means for the translocation of carbon skeletons and energy between source and sink organs as well as being osmoprotective solutes and antioxidants which may be involved in the resistance of some plants to biotic and abiotic stresses. Polyol/Monosaccharide transporter (PLT proteins previously identified in plants are involved in the loading of polyols into the phloem and are reported to be located in the plasma membrane. The functions of PLT proteins in leguminous plants are not yet clear. In this study, a total of 14 putative PLT genes (LjPLT1-14 were identified in the genome of Lotus japonicus and divided into 4 clades based on phylogenetic analysis. Different patterns of expression of LjPLT genes in various tissues were validated by qRT-PCR analysis. Four genes (LjPLT3, 4, 11, and 14 from clade II were expressed at much higher levels in nodule than in other tissues. Moreover, three of these genes (LjPLT3, 4, and 14 showed significantly increased expression in roots after inoculation with Mesorhizobium loti. Three genes (LjPLT1, 3, and 9 responded when salinity and/or osmotic stresses were applied to L. japonicus. Transient expression of GFP-LjPLT fusion constructs in Arabidopsis and Nicotiana benthamiana protoplasts indicated that the LjPLT1, LjPLT6 and LjPLT7 proteins are localized to the plasma membrane, but LjPLT2 (clade IV, LjPLT3, 4, 5 (clade II and LjPLT8 (clade III proteins possibly reside in the Golgi apparatus. The results suggest that members of the LjPLT gene family may be involved in different biological processes, several of which may potentially play roles in nodulation in this nitrogen-fixing legume.

  17. Two putative-aquaporin genes are differentially expressed during arbuscular mycorrhizal symbiosis in Lotus japonicus

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    Giovannetti Marco

    2012-10-01

    Full Text Available Abstract Background Arbuscular mycorrhizas (AM are widespread symbioses that provide great advantages to the plant, improving its nutritional status and allowing the fungus to complete its life cycle. Nevertheless, molecular mechanisms that lead to the development of AM symbiosis are not yet fully deciphered. Here, we have focused on two putative aquaporin genes, LjNIP1 and LjXIP1, which resulted to be upregulated in a transcriptomic analysis performed on mycorrhizal roots of Lotus japonicus. Results A phylogenetic analysis has shown that the two putative aquaporins belong to different functional families: NIPs and XIPs. Transcriptomic experiments have shown the independence of their expression from their nutritional status but also a close correlation with mycorrhizal and rhizobial interaction. Further transcript quantification has revealed a good correlation between the expression of one of them, LjNIP1, and LjPT4, the phosphate transporter which is considered a marker gene for mycorrhizal functionality. By using laser microdissection, we have demonstrated that one of the two genes, LjNIP1, is expressed exclusively in arbuscule-containing cells. LjNIP1, in agreement with its putative role as an aquaporin, is capable of transferring water when expressed in yeast protoplasts. Confocal analysis have demonstrated that eGFP-LjNIP1, under its endogenous promoter, accumulates in the inner membrane system of arbusculated cells. Conclusions Overall, the results have shown different functionality and expression specificity of two mycorrhiza-inducible aquaporins in L. japonicus. One of them, LjNIP1 can be considered a novel molecular marker of mycorrhizal status at different developmental stages of the arbuscule. At the same time, LjXIP1 results to be the first XIP family aquaporin to be transcriptionally regulated during symbiosis.

  18. Symbolic structure in the architecture of the temple – introduction into theology of the sacred art

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    Jerzy Uścinowicz

    2014-11-01

    Full Text Available Symbol has always been an intrinsic part of a person. The human being – homo religiosus – is by nature also a homo symbolicus, who thinks and feels symbolically, who lives symbolically. In the domain of sacrum, in the temple, life is realized through holy symbols.In the past, this was directly reflected in the architecture and in the art of all religions. They have their special compensation in the temple and vice versa; the temple is a concrete manifestation of the function of a symbol. Thanks to them, art could manifest itself, could naturally pass from the level of aesthetics to the level of religion. Nowadays we face a kind of crisis of symbol in the sphere of art, certain reluctance towards symbols. The language of symbols seems to be dying out.Two thousand years of history of Christianity proved that a main criterion of a value of church architecture was not based on architectural precursors. This architecture was sacred because it was a carrier of a „truth of God” and – like a liturgical mysterion and iconography art – it was a theological comment. It was a codified language of the transposes of religions essences and orders, into the form of architectural expression. This was in a East Christianity and this happens there up to this day.One of the proofs to confirmate this thesis is an example of dome. It has been in existence since the beginning of forming the traditional architecture structure of the orthodox temple; it manifested symbolical and archetype essence – as an interior space and as an exterior form. In the history of architecture as well as the history of religion it had precisely defined symbolic meanings. They designated its significance in the temple, they gave rise to its long duration in the history, and eventually they gave it a status of an essential element, an everlasting witness of Divine mystery”. Presentation of this essence and orders constructs indispensable context to a value of the

  19. Snail Darters and Sacred Places: Creative Application of the Endangered Species Act

    Science.gov (United States)

    Gilmer, Robert Andrew

    2013-11-01

    Rather than exploring how indigenous people have been alienated from resources by environmental policies, this paper explores how indigenous peoples have worked with environmental organizations to use the broad protections provided by environmental laws to protect cultural resources. The Eastern Band of Cherokee Indians, along with other concerned groups, partnered with environmentalists in opposing the destruction of the endangered snail darter’s critical habitat by the Tennessee Valley Authority’s Tellico Dam. The dam had been opposed by a shifting alliance of Cherokees, local farmers, trout fisherman, and environmentalists since it was announced in 1963. A previous lawsuit by this coalition delayed the project from 1972 to 1974 under the National Environmental Policy Act. The Endangered Species Act provided this coalition with a powerful tool for opposing the destruction of burial grounds and sacred village sites throughout the lower Little Tennessee River valley. The coalition of environmental organizations, Cherokees, and others was ultimately unsuccessful in stopping the dam from being built, but was successful in establishing a strict precedent for the enforcement of the Endangered Species Act. The lawsuit also created a space for the Eastern Band to negotiate for the return of Cherokee remains and halt the removal of any additional burials. In this situation, the strategic support of environmental regulation enabled the Eastern Band to exert some degree of control over the fate of cultural resources in the valley, and also demonstrates the significant role American Indian peoples played in one of the seminal events of the environmental movement during the 1970s.

  20. Integrating Sacred Knowledge for Conservation: Cultures and Landscapes in Southwest China

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    Jianchu Xu

    2005-12-01

    Full Text Available China is undergoing economic growth and expansion to a free market economy at a scale and pace that are unprecedented in human history. This is placing great pressure on the country's environment and cultural diversity. This paper examines a number of case histories in China, focusing on the culturally varied and ecologically diverse southwest region of the country. We show how developments in recent Chinese history have devalued and in some cases eliminated indigenous knowledge and practices in the quest to strengthen the centralized state. Despite these changes, more than 30 ethnic minorities live in southwest China. For generations these peoples have maintained landscapes through traditional land use and cultural practices. This indigenous knowledge places a high value on protecting forests, landscapes, and water catchments while preserving biodiversity. These values are maintained through religious beliefs, hunting taboos, and the protection of sacred sites. We advocate a conservation policy for China that includes the indigenous knowledge and values needed to maintain the environment and the traditional cultures themselves. There are seminal signs that the government is beginning to support indigenous cultures in China. The Organic Law of 1998 granted villages the legal right to self-government and gave indigenous communities greater responsibility for land and resource use. Traditional and indigenous cultural products have also developed a market and an economic value within a growing tourism industry. In many cases, however, indigenous people remain isolated from major land-use and conservation decisions that are the result of centrally planned policy. Meanwhile, frequent oscillations in forest policy and land tenure insecurity since the 1950s have led to the erosion of many local institutions and the loss of indigenous knowledge. We suggest that the long-term viability of the environment requires an interactive approach that involves

  1. The Ganges and the GAP: An Assessment of Efforts to Clean a Sacred River

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    Kenneth R. Tamminga

    2012-07-01

    Full Text Available For centuries, the Ganges River in India has been the locus of sacred rites for the Hindus. The religious significance of the Ganges is physically manifested in ghats (stepped landings that form the land-water interface. Besides serving as a site for religious bathing and cremation, the ghats are also tied to people’s livelihoods and are an inseparable part of their daily lives. Today, the increasingly urbanized Ganges basin sustains more than 40 percent of India’s population. At the same time, industrialization and the pressures of a growing population along its banks have contributed to alarming levels of pollution in the river. In 1985, the federal government of India launched the Ganga Action Plan (GAP with the primary objective of cleaning the river. However, characterized by centralized planning and control with little public participation, the GAP had limited impact. In 2011, the government launched yet another clean up program—the National Ganga River Basin Project—with support from the World Bank. In this paper, we take a closer look at the programs to highlight the tenuous relationship between the need for ‘efficient’ management of environmental problems and public participation. Can public participation fit into the technocratic model that is often adopted by environmental programs? What approaches to participation kindle authorship and empowerment among those who share a deep relationship with the river and the ghats? Can religious practices be accommodated within scientific frameworks of adaptive management and resilience? We argue that rethinking the relationship between pollution control programs and participation is crucial for any effort to clean the Ganges, restore its waterfront, and catalyze broader regeneration in the Ganges basin.

  2. A sacred command of reason? Deceit, deception, and dishonesty in nurse education.

    Science.gov (United States)

    Rolfe, Gary

    2016-07-01

    Kant (Grounding for the Metaphysics of Morals. Hackett, Indianapolis, 1797) described honesty as 'a sacred command of reason' which should be obeyed at all times and at any cost. This study inquires into the practice of dishonesty, deception, and deceit by universities in the UK in the pursuit of quality indicators such as league table positions, Research Excellence Framework (REF) scores, and student satisfaction survey results. Deception occurs when the metrics which inform these tables and surveys are manipulated to suggest an improvement in quality when, in fact, the raised scores are merely the result of clever strategic planning. Deceit occurs when these manipulated scores are deliberately and knowingly presented as real improvements in research and educational quality. It might be argued that, within the context of the artificial ivory tower world of academe, this is a game played by almost every academic in every higher education institution with no real losers and little wider consequence. However, this study suggests that some of the strategies employed by institutions to improve their scores without directly addressing the issue of quality can, in certain practice-based disciplines such as nursing, result in dire consequences for practitioners and service users. It concludes with a number of suggestions taken from personal experience to resolve the tension between the contractual demands placed on nurse academics by their employers and the moral and practical obligations of their professional body, most notably the use of subversion. The conclusion, contra Kant, is that the most effective strategy against dishonesty and deception is often more dishonesty and deception. © 2016 John Wiley & Sons Ltd.

  3. St Maxim the Greek: Some notes on his understanding of the sacred time

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    Neža Zajc

    2016-10-01

    Full Text Available St Maxim the Greek: Some notes on his understanding of the sacred time Based on a manuscript by St Maxim the Greek, this article explores his specific under­standing of the relationship between language and biblical tradition. It gives some answers to questions concerning his theology, which are posed by his liturgical experience of the sacred time, which is based not on repeating the excerptions from the patristic authors, but is primarily founded on his accurate reading and in-depth perception of the Holy Bible. Maxim the Greek, who in his personal writings showed a detailed knowledge of both the Old Testament and Sla­vonic biblical texts, was thus not only able to separate the canonical from the non-canonical sacred texts, but also successfully classified the Christian teachings according to ethical value, from the Old Testament prophets to the apostles and the Church Fathers. With his hierarchy he also gave meaning to the ontological-eschatological dimension (three levels – appropriate to the Holy Trinity of their spiritual efforts. His knowledge, which also reflects the precise understanding of dogmatic theological decisions of the first ecumenical church councils, ranks highest the learning that comes directly from the Son of God, which Maxim the Greek experienced through his theological-liturgical prayer practice. Maxim found theologically unambiguous formulations which most profoundly deter­mined the specific nature of his personal theology in the Byzantine hymnography dedicated to the Mother of God. All the mentioned facts lead the author to the further explore his specific Old Church Slavonic language, in which he managed to preserve not only the early Christian mentality but also the theological-liturgical characteristics of the ascetic and later monastic discipline that he learned in the monastery of Vatopedi at the Holy Mount Athos. The article concludes with the proposition that only through detailed study of the personal

  4. Diversité et enjeux de conservation des bois sacrés en société ...

    African Journals Online (AJOL)

    Au Burkina Faso, la société Mossi a une longue tradition de pratique animiste qui tend à régresser avec la poussée des religions importées comme l'islam et le christianisme. Les vestiges de cette tradition ancestrale que sont les bois sacrés, témoins de l'élément forestier, sont de plus en plus menacés par la forte densité ...

  5. Desiring the death-of-the-death. Michelangelo (madrigal n.118), Shakespeare (son. 146), Donne (sacred son. X)

    OpenAIRE

    Ida Campeggiani

    2013-01-01

    Michelangelo wrote two versions of the madrigal n. 118; the second one transforms the meaning of the first from the profane to the sacred, with minor variants. Both are included in the archive of texts known as ‘canzoniere’. An analysis of the two shows Michelangelo’s intention to introduce private drives in his work, taking care, though, of creating a framework within which to absorb those subversive aspects the prevailing morality would have rejected. Buonarroti uses the topos of the lover’...

  6. Sacred Practices and Family Processes in a Jewish Context: Shabbat as the Weekly Family Ritual Par Excellence.

    Science.gov (United States)

    Marks, Loren D; Hatch, Trevan G; Dollahite, David C

    2017-03-19

    The present article provides a deep and more focused look at the utility, meaning, processes, and power involved in a specific, family-level, sacred practice or ritual from Judaism: Shabbat (Sabbath). Content analysis of in-depth interviews with 30 diverse, marriage-based Jewish families living in the United States (N = 77 individuals) yielded three emergent themes: (a) "Shabbat brings us closer together"; (b) How Shabbat brings the family together; and (c) The Power of Blessing the Children. These themes will be discussed respectively, along with related verbatim data from participants' in-depth qualitative interviews. © 2017 Family Process Institute.

  7. Diagnosis and Management of Spontaneously Recanalized Coronary Thrombus Guided by Optical Coherence Tomography - Lessons From the French "Lotus Root" Registry.

    Science.gov (United States)

    Souteyrand, Géraud; Valladier, Mathieu; Amabile, Nicolas; Derimay, François; Harbaoui, Brahim; Leddet, Pierre; Barnay, Pierre; Malcles, Guilhem; Mulliez, Aurelien; Berry, Colin; Eschalier, Romain; Combaret, Nicolas; Motreff, Pascal

    2018-02-23

    Spontaneous reanalyzed coronary thrombus (SRCT) has been reported in autopsy series, but little is known about SRCT, and it is potentially under-diagnosed in clinical practice.Methods and Results:SRCT identified on OCT were included in a French multicenter series, the Lotus Root French Registry. A total of 34 SRCT were identified on OCT in 33 patients (23 male; median age, 56 years; IQR, 52-65 years); 23/33 patients (70%) presented with angina pectoris and/or dyspnea. Three angiographic aspects were distinguished retrospectively: braided, pseudo-dissected, and hazy. Stenosis severity on quantitative coronary analysis varied between 11% and 100% (median, 45%), whereas the reduction in lumen area on OCT varied between 20% and 92% (median, 68%). A typical "lotus root" aspect was confirmed on OCT, consisting of multiple circular concave-edged channels of varying size, numbering between 3 and 12 depending on the slice, separated by smooth-edged septa of high luminosity without posterior attenuation. OCT also served to guide treatment, with stenting in 91% of cases. During the 17-month follow-up 91% of patients had excellent evolution. One death and 3 ACS events occurred. In this large SRCT cohort, angiography had limited diagnostic value whereas OCT could be used to define disease characteristics and guide treatment of lesions inducing angina pectoris and/or silent myocardial ischemia. OCT-guided management was associated with good prognosis.

  8. Protective effect of Zizyphus lotus jujube fruits against cypermethrin-induced oxidative stress and neurotoxicity in mice.

    Science.gov (United States)

    Abdelhafidh, Khazri; Mhadhbi, Lazher; Mezni, Ali; Badreddine, Sellami; Beyrem, Hamouda; Mahmoudi, Ezzeddine

    2018-03-01

    Cypermethrin (CYP) is a synthetic pyrethroid insecticide used worldwide in agriculture, home pest control. The toxicity of CYP is well studied in many organisms. The aim of present study was to investigate the protective effect of Zizyphus lotus (Zizyp) fruit against neurotoxicity and oxidative stress induced by CYP in mice. Mice were divided into four groups of six each: groups I and II were used as control and CYP control (20 mg/kg body weight). While, groups III was orally treated with Zizyphus lotus fruit (5 g/kg body weight) plus CYP (20 mg/kg body weight) for 18 days. Furthermore, HPLC-ESI-MS-MS (Q-Tof) and GC-MS were used to identify the compounds fraction. Antioxidant enzyme catalase (CAT), neurotoxicity enzyme acetylcholinesterase (AChE) activities and hydrogen peroxide (H 2 O 2 ) , malondialdehyde (MDA) levels were determined in the liver, kidney and heart. CYP caused decreased CAT activity, inhibition of AChE activity and increased the levels of H 2 O 2 and MDA in heart, liver and kidney. Our results indicate that Zizyp fruit is markedly effective in protecting mice against CYP-induced biochemical changes. This protection may be due to its antioxidant property and scavenging ability against active free radicals.

  9. Green synthesis of nitrogen-doped carbon dots from lotus root for Hg(II) ions detection and cell imaging

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Dan; Shang, Shaoming, E-mail: smshang@jiangnan.edu.cn; Yu, Qin; Shen, Jie

    2016-12-30

    Graphical abstract: Fluorescent nitrogen-doped carbon dots were synthesized by a facile, green, and fast microwave method, using lotus root as carbon source. These nitrogen-doped carbon dots can be used for Hg{sup 2+} detection and cell imaging. - Highlights: • A green, fast strategy for synthesizing carbon dots was established. • A simple, sensitive, selective and wide linear range sensing of Hg{sup 2+} was developed. • The sensor system was demonstrated to detect Hg{sup 2+} in environmental water sample. • The carbon dots could serve for multicolor fluorescence bioimaging. - Abstract: Herein, a facile, green, and fast method was developed in the synthesis of fluorescent nitrogen-doped carbon dots (CDs) with nitrogen content of 5.23%, using one-pot microwave treatment of lotus root (LR), without using any other surface passivation agents. The results show that these LR-CDs (with an average diameter of 9.41 nm) possess many outstanding features and have a high quantum yield of 19.0%. We further demonstrated applications of LR-CDs as probes for heavy metal ion detection. The LR-CDs exhibit captivating sensitivity and selectivity toward Hg{sup 2+} with a linear range from 0.1 to 60.0 μM and a detection limit of 18.7 nM. Eventually, the LR-CDs were applied for multicolor cell imaging, demonstrating their potential toward diverse applications.

  10. A Conceptual Framework for the Electronic Performance Support Systems within IBM Lotus Notes 6 (LN6 Example

    Directory of Open Access Journals (Sweden)

    Servet BAYRAM

    2005-10-01

    Full Text Available A Conceptual Framework for the Electronic PerformanceSupport Systems within IBM Lotus Notes 6 (LN6 Example Assoc. Prof. Dr. Servet BAYRAM Computer Education & Instructional Technologies Marmara University, TURKEYsbayram@marmara.edu.tr ABSTRACT The concept of Electronic Performance Support Systems (EPSS is containing multimedia or computer based instruction components that improves human performance by providing process simplification, performance information and decision support system. EPSS has become a hot topic for organizational development, human resources, performance technology, training, and educational development professionals. A conceptual framework of EPSS is constructed under five interrelated and interdependent domains for educational implications. The domains of the framework are online collaboration, cost-effectiveness, motivation, service management, and performance empowering. IBM Lotus Notes 6 (LN6 is used as an example application tool to illustrate the power of this framework. The framework describes a set of relevant events based upon deductive analyses for improving our understanding of the EPSS and its implications on education and training. The article is also pointed out that there are some similarities between the EPSS’ and the LN6’s specific features within this conceptual framework. It can provide some guidelines and benefits to researchers, educators, and designers as well.

  11. How planting configuration influences plant secondary metabolites and total N in tall fescue (Festuca arundinacea Schreb.), alfalfa (Medicago sativa L.) and birdsfoot trefoil (Lotus corniculatus L.)

    Science.gov (United States)

    Theories suggest that incorporating alfalfa (Medicago sativa L.; Alf) or birdsfoot trefoil (Lotus corniculatus L.; BFT) into endophyte-infected tall fescue (Festuca arundinaceas Schreb.; E+TF) pasturelands may improve livestock production. We investigated how planting configuration might influence p...

  12. Tales of Recombinant Femininity: "The Reincarnation of Golden Lotus," the "Chin P'ing Mei," and the Politics of Melodrama in Hong Kong.

    Science.gov (United States)

    Fore, Steve

    1993-01-01

    Examines the film "The Reincarnation of Golden Lotus" and the text upon which it is loosely based, the "Chin P'ing Mei," and the relationship between it and modern Chinese culture. States that the film's screenplay is much less an adaptation of the source novel than it is a female-centered reworking of a particular narrative…

  13. Long-term outcome of microscopic esophagitis in chronic GERD patients treated with esomeprazole or laparoscopic antireflux surgery in the LOTUS trial

    DEFF Research Database (Denmark)

    Fiocca, Roberto; Mastracci, Luca; Engström, Cecilia

    2010-01-01

    OBJECTIVES: Gastroesophageal reflux disease (GERD)-associated changes in esophageal histology have been reported mainly after short-term medical antireflux therapy, and few individual lesions have been examined. We report detailed histological findings from the LOTUS study, at baseline and at 1 a...

  14. Florescimento e produção de sementes de Lotus subbiflorus Lag. cv. El Rincón Flowering and seed production of Lotus subbiflorus Lag. cv. El Rincón

    Directory of Open Access Journals (Sweden)

    Priscila Silva da Costa Ferreira Gomes

    2011-05-01

    Full Text Available Objetivou-se com este trabalho avaliar a dinâmica do florescimento e o potencial de produção de sementes de Lotus subbiflorus Lag. cv. El Rincón. Por meio de amostragens semanais realizadas entre 13/11/2008 e 4/2/2009, estudaram-se as variáveis número de botões florais, número de inflorescências, número de flores, número de legumes totais, peso de mil sementes e produção de sementes. O delineamento experimental utilizado foi de blocos casualizados com os tratamentos arranjados como níveis de um fator experimental, constituído pelas datas após a semeadura. Dessa forma, o experimento foi realizado com dois blocos e doze unidades experimentais por bloco. A produção de sementes de L. subbiflorus no Sul do Brasil tem problemas decorrentes do florescimento contínuo, o que dificulta a colheita. A produção máxima obtida foi de 63,2 kg/ha em janeiro de 2009 (4372 graus-dia. O peso de mil sementes e o número de legumes foram as características que mais se correlacionaram com a produção de sementes. A emissão constante de botões florais contribui negativamente sobre os demais componentes da produção de sementes.The aim of this work was to evaluate the dynamic of the flowering and the Lotus subbiflorus Lag. Cv. "El Rincón"seed production potential. The variables studied through weekly samples from 11/13/2008 to 02/04/2009 were: number of flower buds, number of inflorescences, number of flowers, number of total legumes, weight of 1000 seeds and seed production. The experimental design used randomized blocks with treatments arranged as levels of an experimental factor, defined by the dates after seeding. The experiment was conducted with two blocks and twelve experimental units per block. Lotus subbiflorus seed production, in the South Brazil, presents problems caused by continuous flowering, difficulting to harvesting. The maximum production obtained was 63.2 kg/ha in January 2009 (4372 degree days. The weight of 1000 seeds and

  15. The Sacred Mountain of Varallo in Italy: seismic risk assessment by acoustic emission and structural numerical models.

    Science.gov (United States)

    Carpinteri, Alberto; Lacidogna, Giuseppe; Invernizzi, Stefano; Accornero, Federico

    2013-01-01

    We examine an application of Acoustic Emission (AE) technique for a probabilistic analysis in time and space of earthquakes, in order to preserve the valuable Italian Renaissance Architectural Complex named "The Sacred Mountain of Varallo." Among the forty-five chapels of the Renaissance Complex, the structure of the Chapel XVII is of particular concern due to its uncertain structural condition and due to the level of stress caused by the regional seismicity. Therefore, lifetime assessment, taking into account the evolution of damage phenomena, is necessary to preserve the reliability and safety of this masterpiece of cultural heritage. A continuous AE monitoring was performed to assess the structural behavior of the Chapel. During the monitoring period, a correlation between peaks of AE activity in the masonry of the "Sacred Mountain of Varallo" and regional seismicity was found. Although the two phenomena take place on very different scales, the AE in materials and the earthquakes in Earth's crust, belong to the same class of invariance. In addition, an accurate finite element model, performed with DIANA finite element code, is presented to describe the dynamic behavior of Chapel XVII structure, confirming visual and instrumental inspections of regional seismic effects.

  16. The Sacred Mountain of Varallo in Italy: Seismic Risk Assessment by Acoustic Emission and Structural Numerical Models

    Directory of Open Access Journals (Sweden)

    Alberto Carpinteri

    2013-01-01

    Full Text Available We examine an application of Acoustic Emission (AE technique for a probabilistic analysis in time and space of earthquakes, in order to preserve the valuable Italian Renaissance Architectural Complex named “The Sacred Mountain of Varallo.” Among the forty-five chapels of the Renaissance Complex, the structure of the Chapel XVII is of particular concern due to its uncertain structural condition and due to the level of stress caused by the regional seismicity. Therefore, lifetime assessment, taking into account the evolution of damage phenomena, is necessary to preserve the reliability and safety of this masterpiece of cultural heritage. A continuous AE monitoring was performed to assess the structural behavior of the Chapel. During the monitoring period, a correlation between peaks of AE activity in the masonry of the “Sacred Mountain of Varallo” and regional seismicity was found. Although the two phenomena take place on very different scales, the AE in materials and the earthquakes in Earth’s crust, belong to the same class of invariance. In addition, an accurate finite element model, performed with DIANA finite element code, is presented to describe the dynamic behavior of Chapel XVII structure, confirming visual and instrumental inspections of regional seismic effects.

  17. Viable contribution of Tibetan sacred mountains in southwestern China to forest conservation.

    Science.gov (United States)

    Shen, Xiaoli; Li, Sheng; Wang, Dajun; Lu, Zhi

    2015-12-01

    The Tibetan sacred mountains (TSMs) cover a large area and may represent a landscape-scale conservation opportunity. We compared the conservation value of forests in these mountains with the conservation value of government-established nature reserves and unmanaged open-access areas in Danba County, southwestern China. We used Landsat satellite images to map forest cover and to estimate forest loss in 1974-1989, 1989-1999, and 1999-2013. The TSMs (n = 41) and nature reserves (n = 4) accounted for 21.6% and 29.7% of the county's land area, respectively. Remaining land was open-access areas (i.e., areas without any restrictions on resource use) (56.2%) and farmlands (2.2%). Within the elevation range suitable for forests, forest cover did not differ significantly between nature reserves (58.8%) and open-access areas (58.4%), but was significantly higher in TSMs (65.5%) after controlling for environmental factors such as aspect, slope, and elevation. The TSMs of great cultural importance had higher forest cover, but patrols by monastery staff were not necessarily associated with increased forest cover. The annual deforestation rate in nonsacred areas almost tripled in 1989-1999 (111.4 ha/year) relative to 1974-1989 (40.4 ha/year), whereas the rate in TSMs decreased in the later period (19.7 ha/year vs. 17.2 ha/year). The reduced forest loss in TSMs in 1989-1999 was possibly due to the renaissance of TSM worship and strengthened management by the local Buddhist community since late 1980s. The annual deforestation rate in Danba decreased dramatically to 4.4 ha/year in 1999-2013, which coincided with the implementation of a national ban on logging in 1998. As the only form of protected area across the Tibetan region during much of its history, TSMs have positively contributed to conserving forest at a landscape scale. Conservation of TSM forests largely relied on the strength of local religious institutions. Integrating community-based conservation of TSMs within the

  18. Importance of Contrast Aortography With Lotus Transcatheter Aortic Valve Replacement: A Post Hoc Analysis From the RESPOND Post-Market Study.

    Science.gov (United States)

    van Gils, Lennart; Wöhrle, Jochen; Hildick-Smith, David; Bleiziffer, Sabine; Blackman, Daniel J; Abdel-Wahab, Mohamed; Gerckens, Ulrich; Brecker, Stephen; Bapat, Vinayak; Modine, Thomas; Soliman, Osama I; Nersesov, Andrey; Allocco, Dominic; Falk, Volkmar; Van Mieghem, Nicolas M

    2018-01-22

    The aim of this post hoc analysis from the RESPOND (Repositionable Lotus Valve System-Post-Market Evaluation of Real World Clinical Outcomes) post-market study was to assess the final implantation depth on the contrast aortogram after Lotus valve (Boston Scientific, Marlborough, Massachusetts) transcatheter aortic valve replacement (TAVR) and to correlate with permanent pacemaker implantation (PPI) and paravalvular leak (PVL). Contrast aortography allows for the assessment of implantation depth and PVL during and after TAVR. Previous reports suggested an association between final device position and rates of PPI and PVL. The RESPOND study was a prospective, open-label, single-arm study in 41 centers evaluating outcomes after Lotus TAVR in routine clinical practice. Aortograms were collected at the Erasmus Medical Center and analyzed by researchers who were blinded to clinical outcomes. The primary analysis correlated implantation depth with PPI and PVL and required aortograms in a coaxial projection. The relation between implantation depth and need for PPI was assessed by multivariate logistic regression, adjusting for pre-defined confounders. A secondary analysis compared PVL analysis by contrast aortography with transthoracic echocardiography (TTE) performed by the independent core laboratory. A total of 724 angiographic studies were included in this analysis. Mean Lotus implantation depth was 6.67 ± 2.19 mm. The overall PPI rate was 35%. PPI rate was lower with shallow implants (analysis of the RESPOND study PPI was highly correlated with implantation depth, whereas PVL was not. Higher Lotus implantation may reduce need for PPI. Copyright © 2018. Published by Elsevier Inc.

  19. Caracterização de espécies diploides de Lotus em resposta à toxidez por alumínio Characterization of diploid species of Lotus in response to aluminum toxicity

    Directory of Open Access Journals (Sweden)

    Armando Martins dos Santos

    2011-05-01

    Full Text Available Objetivou-se com este trabalho caracterizar espécies diploides, inclusive a espécie modelo Lotus japonicus, e linhas endogâmicas recombinantes do gênero Lotus, quanto à tolerância ao alumínio (Al tóxico utilizando-se solo ácido e solução nutritiva. Os experimentos foram conduzidos em casa-de-vegetação, sendo testadas três espécies diploides (L. japonicus MG-20 e GIFU, L. filicaulis e L. burtii e 180 linhas endogâmicas recombinantes. Nos experimentos com espécies diploides, utilizou-se a alfafa como testemunha sensível e, nos experimentos com linhas endogâmicas recombinantes, utilizou-se a espécie modelo GIFU. Nos experimentos em solo, foram avaliadas características morfológicas da parte aérea e da raiz e, nos experimentos em solução nutritiva, apenas o comprimento e crescimento radicular. As espécies modelo MG-20 e GIFU foram, em geral, as mais produtivas. Os resultados em solução nutritiva seguiram padrão de resposta semelhante ao observado nas avaliações em solo ácido, sendo a espécie MG-20 superior às demais em todas as concentrações de alumínio testadas. Das 180 linhas endogâmicas recombinantes testadas, 24 foram superiores e 39 inferiores à espécie GIFU. A grande diversidade observada nas espécies modelo e nas linhas endogâmicas recombinantes pode auxiliar na futura seleção de genótipos cultivados (tetraploides, uma vez que essas espécies possuem um grupo de marcadores moleculares desenvolvidos que podem ser utilizados na identificação de regiões responsáveis pela maior ou menor tolerância à toxidez por alumínio.The objective of this work was to characterize diploid species, including Lotus japonicus model species and recombinant inbred lines of the Lotus genus for resistance to aluminum (Al toxicity by using acid soil and nutrient solution. The experiments were conducted in greenhouse, being tested three diploid species (L. japonicus MG-20 and GIFU, L. filicaulis and L. burtii, and 180

  20. Mobilising Mother Cabrini's Educational Practice: The Transnational Context of the London School of the Missionary Sisters of the Sacred Heart of Jesus 1898-1911

    Science.gov (United States)

    Williams, Maria Patricia

    2015-01-01

    A schoolteacher from Lombardy, Saint Frances Xavier Cabrini (1850-1917), founded the Institute of Missionary Sisters of the Sacred Heart of Jesus (MSC) in 1880. It was one of the 185 female religious institutes established in Italy in the nineteenth century. In the newly unified Italy, Cabrini found opportunities to formulate progressive Catholic…

  1. Sacred Architecture as a Narrative for Defining Religion, Culture and Social and Educational Change: A Proposal for a Series of Workshops for Educators.

    Science.gov (United States)

    Shields, James J.

    Sacred architecture provides a compelling narrative for explaining the ways religious belief and practice contribute to the construction of self identity and of lived cultural values in all societies. This paper proposes a collaborative and ethnographic research workshop series focused on the intersections among culture, religious meaning and…

  2. 77 FR 39564 - Group Lotus Plc, Receipt of Petition for Temporary Exemption From an Advanced Air Bag Requirement...

    Science.gov (United States)

    2012-07-03

    ...In accordance with the procedures in 49 CFR part 555, Group Lotus Plc has petitioned the agency for a temporary exemption from one advanced air bag requirement of FMVSS No. 208, the higher maximum speed (56 km/h (35 mph)) belted test requirement using 5th percentile adult female dummies for its Evora model. The basis for the application is that the petitioner avers compliance would cause it substantial economic hardship and that it has tried in good faith to comply with the standard.\\1\\ This notice of receipt of an application for a temporary exemption is published in accordance with statutory and administrative provisions. NHTSA has made no judgment on the merits of the application. ---------------------------------------------------------------------------

  3. Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

    Science.gov (United States)

    Hiraoka, Yukihiro; Ueda, Hiroaki; Sugimoto, Yukihiro

    2009-01-01

    Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

  4. Substrate-independent sequential deposition process to obtain the lotus effect based on mussel-inspired polydopamine

    Science.gov (United States)

    Ou, Junfei; Pan, Bing; Chen, Yiwei; Xie, Chan; Xue, Mingshan; Wang, Fajun; Li, Wen

    2015-02-01

    A substrate-independent route to achieve the lotus effect on a variety of substrates is proposed based on mussel-inspired polydopamine (PDA), and was tested on titanium alloy, polypropylene and silicon substrates. The substrates were firstly coated with PDA and then sequentially transferred to aqueous CuCl2 and AgNO3 solutions for copper and silver deposition. Finally, the samples were passivated by the low-surface-energy molecules of 1-dodecanethiol, and surface superhydrophobicity (contact angle > 160°; sliding angle between 1° and 2°) could be obtained. Due to the strong adhesion of PDA to a wide range of materials, it is expected that this deposition process can be applied to a variety of other substrates including metal, polymer, and inorganic-nonmetallic materials.

  5. Green synthesis of nitrogen-doped carbon dots from lotus root for Hg(II) ions detection and cell imaging

    Science.gov (United States)

    Gu, Dan; Shang, Shaoming; Yu, Qin; Shen, Jie

    2016-12-01

    Herein, a facile, green, and fast method was developed in the synthesis of fluorescent nitrogen-doped carbon dots (CDs) with nitrogen content of 5.23%, using one-pot microwave treatment of lotus root (LR), without using any other surface passivation agents. The results show that these LR-CDs (with an average diameter of 9.41 nm) possess many outstanding features and have a high quantum yield of 19.0%. We further demonstrated applications of LR-CDs as probes for heavy metal ion detection. The LR-CDs exhibit captivating sensitivity and selectivity toward Hg2+ with a linear range from 0.1 to 60.0 μM and a detection limit of 18.7 nM. Eventually, the LR-CDs were applied for multicolor cell imaging, demonstrating their potential toward diverse applications.

  6. Genetic Screening Identifies Cyanogenesis-Deficient Mutants of Lotus japonicus and Reveals Enzymatic Specificity in Hydroxynitrile Glucoside Metabolism

    DEFF Research Database (Denmark)

    Takos, A.; Lai, D.; Mikkelsen, L.

    2010-01-01

    Cyanogenesis, the release of hydrogen cyanide from damaged plant tissues, involves the enzymatic degradation of amino acid-derived cyanogenic glucosides (alpha-hydroxynitrile glucosides) by specific beta-glucosidases. Release of cyanide functions as a defense mechanism against generalist herbivores....... We developed a high-throughput screening method and used it to identify cyanogenesis deficient (cyd) mutants in the model legume Lotus japonicus. Mutants in both biosynthesis and catabolism of cyanogenic glucosides were isolated and classified following metabolic profiling of cyanogenic glucoside...... content. L. japonicus produces two cyanogenic glucosides: linamarin (derived from Val) and lotaustralin (derived from Ile). Their biosynthesis may involve the same set of enzymes for both amino acid precursors. However, in one class of mutants, accumulation of lotaustralin and linamarin was uncoupled...

  7. Lotus japonicus flowers are defended by a cyanogenic β-glucosidase with highly restricted expression to essential reproductive organs

    DEFF Research Database (Denmark)

    Lai, Daniela; Pičmanová, Martina; Abou Hachem, Maher

    2015-01-01

    Flowers and leaves of Lotus japonicus contain α-, β-, and γ-hydroxynitrile glucoside (HNG) defense compounds, which are bioactivated by β-glucosidase enzymes (BGDs). The α-HNGs are referred to as cyanogenic glucosides because their hydrolysis upon tissue disruption leads to release of toxic...... hydrogen cyanide gas, which can deter herbivore feeding. BGD2 and BGD4 are HNG metabolizing BGD enzymes expressed in leaves. Only BGD2 is able to hydrolyse the α-HNGs. Loss of function mutants of BGD2 are acyanogenic in leaves but fully retain cyanogenesis in flowers pointing to the existence...... of an alternative cyanogenic BGD in flowers. This enzyme, named BGD3, is identified and characterized in this study. Whereas all floral tissues contain α-HNGs, only those tissues in which BGD3 is expressed, the keel and the enclosed reproductive organs, are cyanogenic. Biochemical analysis, active site architecture...

  8. Root growth of Lotus corniculatus interacts with P distribution in young sandy soil

    Directory of Open Access Journals (Sweden)

    B. Felderer

    2013-03-01

    Full Text Available Large areas of land are restored with unweathered soil substrates following mining activities in eastern Germany and elsewhere. In the initial stages of colonization of such land by vegetation, plant roots may become key agents in generating soil formation patterns by introducing gradients in chemical and physical soil properties. On the other hand, such patterns may be influenced by root growth responses to pre-existing substrate heterogeneities. In particular, the roots of many plants were found to preferentially proliferate into nutrient-rich patches. Phosphorus (P is of primary interest in this respect because its availability is often low in unweathered soils, limiting especially the growth of leguminous plants. However, leguminous plants occur frequently among the pioneer plant species on such soils, as they only depend on atmospheric nitrogen (N fixation as N source. In this study we investigated the relationship between root growth allocation of the legume Lotus corniculatus and soil P distribution on recently restored land. As test sites, the experimental Chicken Creek Catchment (CCC in eastern Germany and a nearby experimental site (ES with the same soil substrate were used. We established two experiments with constructed heterogeneity, one in the field on the experimental site and the other in a climate chamber. In addition, we conducted high-density samplings on undisturbed soil plots colonized by L. corniculatus on the ES and on the CCC. In the field experiment, we installed cylindrical ingrowth soil cores (4.5 × 10 cm with and without P fertilization around single two-month-old L. corniculatus plants. Roots showed preferential growth into the P-fertilized ingrowth-cores. Preferential root allocation was also found in the climate chamber experiment, where single L. corniculatus plants were grown in containers filled with ES soil and where a lateral portion of the containers was additionally supplied with a range of different P

  9. Root growth of Lotus corniculatus interacts with P distribution in young sandy soil

    Science.gov (United States)

    Felderer, B.; Boldt-Burisch, K. M.; Schneider, B. U.; Hüttl, R. F. J.; Schulin, R.

    2013-03-01

    Large areas of land are restored with unweathered soil substrates following mining activities in eastern Germany and elsewhere. In the initial stages of colonization of such land by vegetation, plant roots may become key agents in generating soil formation patterns by introducing gradients in chemical and physical soil properties. On the other hand, such patterns may be influenced by root growth responses to pre-existing substrate heterogeneities. In particular, the roots of many plants were found to preferentially proliferate into nutrient-rich patches. Phosphorus (P) is of primary interest in this respect because its availability is often low in unweathered soils, limiting especially the growth of leguminous plants. However, leguminous plants occur frequently among the pioneer plant species on such soils, as they only depend on atmospheric nitrogen (N) fixation as N source. In this study we investigated the relationship between root growth allocation of the legume Lotus corniculatus and soil P distribution on recently restored land. As test sites, the experimental Chicken Creek Catchment (CCC) in eastern Germany and a nearby experimental site (ES) with the same soil substrate were used. We established two experiments with constructed heterogeneity, one in the field on the experimental site and the other in a climate chamber. In addition, we conducted high-density samplings on undisturbed soil plots colonized by L. corniculatus on the ES and on the CCC. In the field experiment, we installed cylindrical ingrowth soil cores (4.5 × 10 cm) with and without P fertilization around single two-month-old L. corniculatus plants. Roots showed preferential growth into the P-fertilized ingrowth-cores. Preferential root allocation was also found in the climate chamber experiment, where single L. corniculatus plants were grown in containers filled with ES soil and where a lateral portion of the containers was additionally supplied with a range of different P concentrations. In

  10. Analysis of Lotus japonicus nuclear pore NUP107-160 subcomplex mutants reveals pronounced structural plasticity and functional redundancy

    Directory of Open Access Journals (Sweden)

    Andreas eBinder

    2014-01-01

    Full Text Available Mutations in the Lotus japonicus nucleoporin genes, NUP85, NUP133 and NENA (SEH1, lead to defects in plant-microbe symbiotic signaling. The homologous proteins in yeast and vertebrates are part of the conserved NUP84/NUP107-160 sub-complex, which is an essential component of the nuclear pore scaffold and has a pivotal role in NPC assembly. Loss and down-regulation of NUP84/NUP107-160 members has previously been correlated with a variety of growth and molecular defects, however, in L. japonicus only surprisingly specific phenotypes have been reported. We investigated whether Lotus nup85, nup133 and nena mutants exhibit general defects in NPC composition and distribution. Whole mount immunofluorescence confirmed a typical nucleoporin-like localization for NUP133, which was unchanged in the nup85-1 mutant. Severe NPC clustering and aberrations in the nuclear envelope have been reported for Saccharomyces cerevisiae nup85 and nup133 mutants. However, upon transmission electron microscopy analysis, we did not detect any significant changes in L. japonicus nup85 and nena, and only a slight reduction in the average distances between neighboring NPCs in nup133. Using quantitative immunodetection on protein-blots we observed that loss of individual nucleoporins affected the protein levels of other NUP107-160 complex members. Unlike the single mutants, nup85/nup133 double mutants exhibited severe temperature dependent growth and developmental defects, suggesting that the loss of more than one NUP107-160 member affects basal functions of the NPC.

  11. Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stress

    KAUST Repository

    Liu, Junwei

    2015-02-26

    Main conclusion: Strigolactone changes and cross talk with ABA unveil a picture of root-specific hormonal dynamics under stress.Abstract: Strigolactones (SLs) are carotenoid-derived hormones influencing diverse aspects of development and communication with (micro)organisms, and proposed as mediators of environmental stimuli in resource allocation processes; to contribute to adaptive adjustments, therefore, their pathway must be responsive to environmental cues. To investigate the relationship between SLs and abiotic stress in Lotus japonicus, we compared wild-type and SL-depleted plants, and studied SL metabolism in roots stressed osmotically and/or phosphate starved. SL-depleted plants showed increased stomatal conductance, both under normal and stress conditions, and impaired resistance to drought associated with slower stomatal closure in response to abscisic acid (ABA). This confirms that SLs contribute to drought resistance in species other than Arabidopsis. However, we also observed that osmotic stress rapidly and strongly decreased SL concentration in tissues and exudates of wild-type Lotus roots, by acting on the transcription of biosynthetic and transporter-encoding genes and independently of phosphate abundance. Pre-treatment with exogenous SLs inhibited the osmotic stress-induced ABA increase in wild-type roots and down-regulated the transcription of the ABA biosynthetic gene LjNCED2. We propose that a transcriptionally regulated, early SL decrease under osmotic stress is needed (but not sufficient) to allow the physiological increase of ABA in roots. This work shows that SL metabolism and effects on ABA are seemingly opposite in roots and shoots under stress.

  12. Les rapports de l’art abstrait (Kandinsky, Klee, Mondrian avec les tendances d’abstraction de l’art sacré / The Connections of Abstract Art (Kandinsky, Klee, Mondrian with the Abstractization Tendencies of Sacred Art

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    Gabriel Badea

    2016-11-01

    Full Text Available The main purpose of this paper is to study the connections that can be established between the modern abstractionism and the abstract tendencies from other historical eras. In the first part I will present three distinct interpretations: the first direction is based on authors as Mircea Eliade and Roger Lipsey, who see modern art through the links still alive between art and religion, from a syncretic perspective, or, after Eliade’s expression, based on a creative hermeneutics. The second direction is represented by the work of Adorno, Compagnon, Greenberg, Lyotard, for whom the modern art is a manifestation of radical discontinuity in relation to the art of the past, and the emergence of abstractionism is due primarily to a historical necessity (the increasing rupture between form and content, the increased autonomy of the sensible over the intelligible. The third direction is represented to Wilhelm Worringer, whose work (Abstraktion und Einfühlung, 1907 predates the emergence of the first abstract paintings, but relying on the German aesthetic tradition, manages to go beyond the threshold distinction between figurative and abstract, thus identifying a type of Einfühlung art and another of abstract type, namely the predominance of one or the other in different historical contexts and civilizational patterns. In the second part of the paper I will refer to instances of the spirit of abstraction in the case of Byzantine sacred art, especially in the footsteps of Plotinian aesthetics and as a result of the iconoclastic crisis. In the last part, I will present the key ideas for three major representatives of abstractionism (Kandinsky, Klee, Mondrian and the survival of the concepts of sacred art in their works and art theories.

  13. An analysis of synteny of Arachis with Lotus and Medicago sheds new light on the structure, stability and evolution of legume genomes

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    Nielsen Anna M

    2009-01-01

    Full Text Available Abstract Background Most agriculturally important legumes fall within two sub-clades of the Papilionoid legumes: the Phaseoloids and Galegoids, which diverged about 50 Mya. The Phaseoloids are mostly tropical and include crops such as common bean and soybean. The Galegoids are mostly temperate and include clover, fava bean and the model legumes Lotus and Medicago (both with substantially sequenced genomes. In contrast, peanut (Arachis hypogaea falls in the Dalbergioid clade which is more basal in its divergence within the Papilionoids. The aim of this work was to integrate the genetic map of Arachis with Lotus and Medicago and improve our understanding of the Arachis genome and legume genomes in general. To do this we placed on the Arachis map, comparative anchor markers defined using a previously described bioinformatics pipeline. Also we investigated the possible role of transposons in the patterns of synteny that were observed. Results The Arachis genetic map was substantially aligned with Lotus and Medicago with most synteny blocks presenting a single main affinity to each genome. This indicates that the last common whole genome duplication within the Papilionoid legumes predated the divergence of Arachis from the Galegoids and Phaseoloids sufficiently that the common ancestral genome was substantially diploidized. The Arachis and model legume genomes comparison made here, together with a previously published comparison of Lotus and Medicago allowed all possible Arachis-Lotus-Medicago species by species comparisons to be made and genome syntenies observed. Distinct conserved synteny blocks and non-conserved regions were present in all genome comparisons, implying that certain legume genomic regions are consistently more stable during evolution than others. We found that in Medicago and possibly also in Lotus, retrotransposons tend to be more frequent in the variable regions. Furthermore, while these variable regions generally have lower

  14. Rationalization of indigenous male circumcision as a sacred religious custom: health beliefs of Xhosa men in South Africa.

    Science.gov (United States)

    Mavundla, Thandisizwe Redford; Netswera, Fulufelo Godfrey; Bottoman, Brian; Toth, Ferenc

    2009-10-01

    This article presents research findings based on the meaning of indigenous circumcision to Xhosa men in South Africa. In South Africa, male circumcision is a rite of passage from adolescence to adulthood. The country has experienced serious problems associated with the practice of this rite ranging from dehydration to death in the traditional "bush" circumcision schools. A qualitative, endogenous research "How do you experience having a son who is undergoing the circumcision rite?" The study revealed cultural circumcision as a "sacred religious practice" with five themes, namely (a) readiness of Xhosa families to engage in the circumcision ritual, (b) the act of circumcision and preparation for manhood, (c) the importance of symbolic purity during the circumcision ritual, (d) celebrating acquired manhood, and (5) aspects of manhood and the rejection of clinical care. Secondary to this are health promotion recommendations made for individuals involved in this ritual.

  15. Safety assessment of the aqueous extract of the flowers of Nymphaea lotus Linn (Nymphaeaceae): Acute, neuro- and subchronic oral toxicity studies in albinos Wistar rats.

    Science.gov (United States)

    Kameni Poumeni, Mireille; Bilanda, Danielle Claude; Dzeufiet Djomeni, Paul Désiré; Mengue Ngadena, Yolande Sandrine; Mballa, Marguerite Francine; Ngoungoure, Madeleine Chantal; Ouafo, Agnès Carolle; Dimo, Théophile; Kamtchouing, Pierre

    2017-03-24

    Background Nymphaea lotus Linn (N. lotus) is a medicinal plant widely used in Cameroon popular medicine, to treat neuropsychiatric conditions, male sexual disorders or as food supplement. However, scientific data on the pharmacotoxic profile of this plant are not available. The safety of N. lotus was assessed in acute, neuro- and subchronic toxicity studies by following the OECD guidelines. Effectively, no data have been published until now in regard to its safety on the nervous system. Methods Aqueous extract of N. lotus at doses of 200, 400 and 600 mg/kg body weight (BW) was evaluated for nitrites contents and orally administered to rats daily for 28 days (5 male, 5 female per group). The control group received distilled water (10 mL/kg) and a satellite group was used to observe reversal effects. Neurotoxicity of the plant was determined using open field test for motor coordination, ataxia and gait analysis. Clinical signs and state of livelihood were recorded during the 24 h, then for 28 days of treatments. At the end of 28-day period, animals were anesthetized and decapitated. The whole brain was homogenized for neurobiochemical analysis. Blood samples were collected with or without anticoagulant for hematological examinations and serum analysis. Specimens of liver, kidney, testis, ovaries, and brain were fixed in 10 % formalin and processed for histopathological examinations. Results Our findings indicate dose-dependent elevation of nitrites contents in the flowers aqueous extract of N. lotus. Acute toxicity study revealed no signs of toxicity neither at the dose 2,000 mg/kg nor at 5,000 mg/kg. Thus the LD50 value of aqueous extract of N. lotus flowers is superior to 5,000 mg/kg. The repeated administration of N. lotus during 28 days, induced no signs of neurobehavioral changes in male, but female rats exhibited dose-dependent response in the open field test, suggesting sex and dose-relative psychotropic effects of N. lotus. The evaluation of

  16. Statutory Interpretation through the Prism of the Bill of Rights: S v Litako 2014 SACR 431 (SCA

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    Salona Lutchman

    2015-07-01

    Full Text Available On 16 April 2014, the Supreme Court of Appeal handed down judgment in the matter of S v Litako 2014 2 SACR 431 (SCA ("Litako". The judgment reconsiders the landmark decision of the same court, S v Ndhlovu 2002 2 SACR 325 (SCA ("Ndhlovu" in which the court held that an informal admission made by one accused could be admitted against a co-accused even if the accused in court denies making the statement and the statement itself is therefore considered to be hearsay. The court in Ndhlovu applied section 3 of the Law of Evidence Amendment Act 45 of 1988 and found that the hearsay extra curial admission could be admitted in the interests of justice. In Litako the court found that section 3 did not overrule an existing common law rule, which is that the extra curial statement of an accused (whether an informal admission or a confession cannot be tendered against a co-accused. This is because section 3 does not expressly overrule this common law rule. Rather, the provision itself requests that its application be subject to the common law. The judgment is important for various reasons. Firstly, it is generally in keeping with the existing rule on the cautionary treatment of accomplice evidence. Secondly, the judgment highlights the current confusion in the relationship between statute and common law with regards to informal admissions and confessions. Thirdly, the court employs methods of statutory interpretation to re-examine the principle from Ndhlovu and finds that the court in that case did not apply its mind correctly in disregarding the common law rule. The court undertook a teleological approach to interpretation by infusing the meaning of the words with the spirit, purport and objects of the Bill of Rights and found that the statute had not overruled the common law rule.

  17. Responsibility, God and society: The cry of the Other in the sacred texts as a challenge towards responsible global citizenship

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    Johann-Albrecht Meylahn

    2009-04-01

    Full Text Available The article seeks to respond to the question: What role can the sacred texts play in the construction of a Christian identity that is responsible to the Other in a pluralistic global world? The sacred texts of the Judaic-Christian tradition offer not only an understanding of the wholly otherness of God, but also form the basis of our understanding and perception of humanity (anthropology, the world and ourselves (personhood/identity. This understanding is constructed in the context of responding to the call of the wholly Other and the others. Identities are traditionally constructed through the identification and exclusion of differences (otherness, thus leading to an ethic of exclusion and responsibility only to oneself/ourselves. Yet these identity-forming texts harbour a persistent otherness, which challenges these traditional identities by interrupting them with a call to responsibility toward the other. The otherness harboured in these texts takes various forms, namely: The otherness of the ancient world to our world, the otherness of the transcendental Other, and the otherness of the text itself, as there is always a différance that has not yet been heard. These various forms of otherness, of our identity-forming texts, deconstruct our identity constructions, thus calling us to a continuous responsibility towards the other. This call could form the basis of a Christian identity and ethic of global cosmopolitan citizenship that is always responding to the eschatological interruption by the other, who is not yet present or who has not been offered presence.

  18. Predictors of Paravalvular Regurgitation After Implantation of the Fully Repositionable and Retrievable Lotus Transcatheter Aortic Valve (from the REPRISE II Trial Extended Cohort).

    Science.gov (United States)

    Blackman, Daniel J; Meredith, Ian T; Dumonteil, Nicolas; Tchétché, Didier; Hildick-Smith, David; Spence, Mark S; Walters, Darren L; Harnek, Jan; Worthley, Stephen G; Rioufol, Gilles; Lefèvre, Thierry; Houle, Vicki M; Allocco, Dominic J; Dawkins, Keith D

    2017-07-15

    Paravalvular leak (PVL) after transcatheter aortic valve replacement is associated with worse long-term outcomes. The Lotus Valve incorporates an innovative Adaptive Seal designed to minimize PVL. This analysis evaluated the incidence and predictors of PVL after implantation of the Lotus transcatheter aortic valve. The REPRISE II (REpositionable Percutaneous Replacement of Stenotic Aortic Valve through Implantation of Lotus Valve System - Evaluation of Safety and Performance) Study With Extended Cohort enrolled 250 high-surgical risk patients with severe symptomatic aortic stenosis. Aortic regurgitation was assessed by echocardiography pre-procedure, at discharge and 30 days, by an independent core laboratory. Baseline and procedural predictors of mild or greater PVL at 30 days (or at discharge if 30-day data were not available) were determined using a multivariate regression model (n = 229). Of the 229 patients, 197 (86%) had no/trace PVL, 30 had mild, and 2 had moderate PVL; no patient had severe PVL. Significant predictors of mild/moderate PVL included device:annulus area ratio (odds ratio [OR] 0.87; 95% CI 0.83 to 0.92; p 10% annular oversizing by area were 17.5% (11 of 63), 2.9% (2 of 70), and 3.2% (2 of 63), respectively. Significant independent predictors of PVL included device:annulus area ratio and left ventricular outflow tract calcium volume. When the prosthetic valve was oversized by ≥5%, the rate of mild or greater PVL was only 3%. In conclusion, the overall rates of PVL with the Lotus Valve are low and predominantly related to device/annulus areas and calcium; these findings have implications for optimal device sizing. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Study of the Glucidic Fraction of Celtis Australis L, Crataegus Azarolus L, Crataegus Monogyna Jacq., Elaeagnus Angustifolia L. and Zizyphus Lotus L. Fruits

    OpenAIRE

    Saadoudi Mouni; Hamebaba Leila; Abdeddaim Mohamed

    2012-01-01

    In Algeria, some fruit trees produce fruits in free nature. Such trees are Celtis australis, Crataegus azarolus, Crataegus monogyna and Zizyphus lotus. In spite of their appreciable consumption, their nutritional value remains unknown. The objective of this study is the determination of sugars in the pulpe and almond of the above fruits. The biochemical analysis shows that these fruits present interesting contents of soluble sugars which confers significant caloric intakes to them. As well as...

  20. Combined Application of Antibrowning, Heat Treatment and Modified-Atmosphere Packaging to Extend the Shelf Life of Fresh-Cut Lotus Root.

    Science.gov (United States)

    Son, Jihye; Hyun, Jeong-Eun; Lee, Jo-Won; Lee, Sun-Young; Moon, BoKyung

    2015-06-01

    This work aimed to determine the effects of different concentrations of antibrowning treatments (that is, distilled water [DW], 1% ascorbic acid [AA], 0.5% chamomile [CM], and 1% AA + 0.5% CM) and heat-treatment (55 °C for 45 s) combined with packaging under 4 different modified-atmosphere gas compositions (that is, air, vacuum, 100% CO2 , 50% CO2 /50% N2 ) on the quality and microbiological characteristics of fresh-cut lotus root. The quality characteristics (that is, color, weight loss, texture, pH, polyphenoloxidase activity, and total phenolic content) of the AA + CM-dipped sample in 100% CO2 packaging were maintained significantly better than those of the other samples (P < 0.05). The microbiological counts observed in the DW-dipped sample during storage were higher than those of the AA, CM, and AA + CM samples, and heat-treatment retarded the microbiological deterioration of fresh-cut lotus root. Therefore, the results revealed that dipping in an antibrowning treatment (AA + CM), and 100% CO2 MAP with heat treatment effectively extend the shelf life of fresh-cut lotus root to 21 d at 5 °C. © 2015 Institute of Food Technologists®

  1. Comparative evaluation of chemical profiles of three representative 'snow lotus' herbs by UPLC-DAD-QTOF-MS combined with principal component and hierarchical cluster analyses.

    Science.gov (United States)

    Chen, Qi-Lei; Zhu, Lin; Tang, Yi-Na; Kwan, Hiu-Yee; Zhao, Zhong-Zhen; Chen, Hu-Biao; Yi, Tao

    2017-08-01

    Herbal healthcare products are used worldwide as relatively safe and effective alternatives to allopathic drugs. Saussurea laniceps Hand.-Mazz. (SL), S. medusa Maxim. (SM) and S. involucrata (Kar. et Kir.) Sch.Bip. (SI) are three sources of the renowned 'snow lotus', Chinese materia medica for treating inflammatory diseases. The three species have different therapeutic effects, among which SL has been proved to be the most potent, but they are frequently confused on the market and in the academic community. An ultra-high performance liquid chromatography-diode array detector-quadrupole time of flight-mass spectrometry (UPLC-DAD-QTOF-MS) method was developed and used to analyze 49 herbal samples for species analysis and overall quality evaluation. With 25 simultaneously identified constituents, of which 12 were quantified, the three herbs showed different chemical profiles. Four-dimensional principle component analysis (4D-PCA) and orthogonal hierarchical cluster analysis (2D-HCA) results illustrated that SL should be grouped away from SM and SI, contradicting the botanical record in Flora of China. The present chemical determination and pattern recognition results directly explain the therapeutic potency of SL and distinguish the three confused snow lotus herbs. Furthermore, the findings suggest a possible extensive quality evaluation model for multi-origin medicinal plants and help monitor falsification of snow lotus herbal products on the market, contributing to a more regulated pharmaceutical industry. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  2. Controlled synthesis of CeO2 microstructures from 1D rod-like to 3D lotus-like and their morphology-dependent properties

    Science.gov (United States)

    Gong, Jinfeng; Meng, Fanming; Fan, Zhenghua; Li, Huijie

    2016-10-01

    Monodisperse 3D lotus-like CeO2 microstructures have been successfully synthesized via controlling the morphology of CeCO3OH precursors under hydrothermal condition as well as subsequent calcination. The reaction time was systematically investigated. XRD, FT-IR, SEM, TEM, XPS, Raman scattering and Photoluminescence (PL) spectra were employed to characterize the samples. The lotus-like CeO2 hierarchical structures with an average of 4-6 μm are composed of many nanoplates of 100-200 nm in thickness as the petals stacking together to form open flowers and have a fluorite cubic structure. Based on the time-dependent morphology evolution evidences, a nucleation-dissolution-recrystallization mechanism has been proposed to explain the transformation from rod-like structures to lotus-like CeO2 hierarchical structures with the increase of reaction time. It is found that there are Ce3+ ions and oxygen vacancies in surface of samples. The magnetic and photoluminescence measurements indicated that all CeO2 samples exhibit excellent ferromagnetism and optical properties at room temperature, and while increasing the reaction time, the ferromagnetism and optical properties increase more, which can be reasonably explained for the influences of the different morphology of the particles and the concentration of oxygen vacancies and Ce3+ ions. [Figure not available: see fulltext.

  3. Caracterização agronômica de genótipos de cornichão (Lotus corniculatus L. Agronomic characterization if birdsfoot trefoil genotypes (Lotus corniculatus L.

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    Maria Tereza Bolzon Soster

    2004-12-01

    Full Text Available Este trabalho foi conduzido com o objetivo de caracterizar agronomicamente genótipos de cornichão (Lotus corniculatus L., incluindo oito populações do cv. São Gabriel, um cultivar rizomatoso (cv. ARS-2620 e um cultivar sem rizomas (cv. Trueno. Plantas individuais foram cultivadas e submetidas a sete cortes em condições de campo, durante um ano, em Passo Fundo, Rio Grande do Sul, Brasil. Os genótipos apresentaram variabilidade quanto ao comprimento de entrenós, estatura, diâmetro, comprimento de legumes, produção de forragem e valor nutritivo. Na média geral, as populações e o cv. Trueno foram cerca de 50% mais produtivos em relação ao cv. ARS-2620. Os genótipos apresentaram entre 80 e 100% de sobrevivência. O conteúdo de proteína bruta reduziu de 21,8% (estádio vegetativo para 11,2% (estádio de frutificação. A média geral de fibra detergente ácido foi de 24,2% e a de fibra detergente neutro variou entre 55,3 e 58,8%.This work was carried out to characterize agronomically birdsfoot trefoil (Lotus corniculatus L. genotypes, including eight populations derived from the cultivar São Gabriel, one rhizomatous cultivar (ARS-260 and one cultivar without rhizomes (Trueno. Individual plants were submitted to seven cuts under field conditions during one year, at Passo Fundo, Rio Grande do Sul, Brazil. The genotypes showed variability relative to internode lenght, height, diameter, legume lenght, forage production and nutritive value. On the average, the populations and the cv. Trueno were about 50% more productive than the cv. ARS-260. The genotypes presented a survival range from 80% to 100%. The crude protein content decreased from 21.8 (vegetative stage to 11.2% (fruiting stage. The overall mean for acid detergent fiber was 24.2% and the neutral detergent fiber ranged from 55.3% to 58.8%.

  4. Les lieux du sacré dans les Miracula sancti Germani d’Heiric d’Auxerre et les Gesta pontificum Autissiodorensium

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    Dominique Iogna-Prat

    2004-09-01

    Full Text Available Comment cerner la notion de sacré dans les textes carolingiens que sont les Gestes des évêques d’Auxerre et les Miracles de saint Germain écrits par l’un des maîtres de l’école carolingienne d’Auxerre, Heiric ? Une étude lexicale précise des mots latins employés par les auteurs permet d’approcher au plus près les concepts renvoyant aux lieux du sacré. Ce sujet de maîtrise. suggéré par D. Iogna-Prat et réalisé sous la direction de M. Bourin (université de Paris I-Panthéon Sorbonne, avec l’aid...

  5. Poetic imagination: a qualitative study on emotion, images, and verses from sacred texts in the praxis of theological reflection in pastoral care and counseling.

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    O'Connor, Thomas St James; Meakes, Elizabeth

    2008-01-01

    What is the role of emotions, imagination, and images in the praxis of theological reflection in pastoral care and counseling, and what images and/or verses from sacred texts best describe the process of theological reflection? These two questions guided this ethnographic study. Seventy-five practitioners of pastoral care and counseling were interviewed and field notes were also made. Findings include the importance of emotion and imagination with a variety of images and verses from sacred texts. Poetic imagination best describes the process. Discussion involves the implications of the findings with suggestions for teaching, ministry, and areas for future research. Lovers and madmen have such seething brains, Such shaping fantasies, that apprehend More than cool reason ever comprehends The lunatic, the lover, and the poet Are of imagination all compact.... William Shakespeare (1596, [1997]) A Midsummer Night's Dream, Act V, Sc I, 5-9.

  6. Morality and Intimate Partner Violence: Do Men in Court-Mandated Psychological Treatment Hold a Sacred Moral Vision of the World and Themselves?

    Science.gov (United States)

    Vecina, María L; Chacón, José C

    2016-01-01

    This article examines the characterization of men in a court-mandated treatment for violence against their partners as holding a sacred vision of the 5 moral foundations and of their own morality. This characterization is compatible with the assumption that a sacred moral world is easily threatened by reality and that may be associated to violent defensive actions. The results from latent class analyses reveal (a) a 4-class distribution depending exclusively on the intensity with which all participants (violent and nonviolent) tend to sacralize the actions proposed in the Moral Foundations Sacredness Scale and (b) a greater prevalence of the violent participants among the classes that are more prone to sacralize. They also show that they hold an inflated moral vision of themselves: They think they are much more moral than intelligent than others who have never been charged with criminal behavior (Muhammad Ali effect).

  7. In Vitro and In Vivo Characterization of the Alkaloid Nuciferine.

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    Martilias S Farrell

    Full Text Available The sacred lotus (Nelumbo nucifera contains many phytochemicals and has a history of human use. To determine which compounds may be responsible for reported psychotropic effects, we used in silico predictions of the identified phytochemicals. Nuciferine, an alkaloid component of Nelumbo nucifera and Nymphaea caerulea, had a predicted molecular profile similar to antipsychotic compounds. Our study characterizes nuciferine using in vitro and in vivo pharmacological assays.Nuciferine was first characterized in silico using the similarity ensemble approach, and was followed by further characterization and validation using the Psychoactive Drug Screening Program of the National Institute of Mental Health. Nuciferine was then tested in vivo in the head-twitch response, pre-pulse inhibition, hyperlocomotor activity, and drug discrimination paradigms.Nuciferine shares a receptor profile similar to aripiprazole-like antipsychotic drugs. Nuciferine was an antagonist at 5-HT2A, 5-HT2C, and 5-HT2B, an inverse agonist at 5-HT7, a partial agonist at D2, D5 and 5-HT6, an agonist at 5-HT1A and D4 receptors, and inhibited the dopamine transporter. In rodent models relevant to antipsychotic drug action, nuciferine blocked head-twitch responses and discriminative stimulus effects of a 5-HT2A agonist, substituted for clozapine discriminative stimulus, enhanced amphetamine induced locomotor activity, inhibited phencyclidine (PCP-induced locomotor activity, and rescued PCP-induced disruption of prepulse inhibition without induction of catalepsy.The molecular profile of nuciferine was similar but not identical to that shared with several approved antipsychotic drugs suggesting that nuciferine has atypical antipsychotic-like actions.

  8. The Spirituality of the Leader and its influence on Visitor Experience Management at Sacred Sites in the Island of Ireland: Insights and implications

    OpenAIRE

    Enongene, Vreny; Griffin, Kevin A.

    2017-01-01

    Despite growing interest in understanding the sacred site visitor experience management, scholars have predominantly explored the phenomenon from the visitor’s perspective. There is very little exploration from the managerial perspectives, given that decisions regarding the nature of the experience, the product and service delivery strategies aimed at providing a diversity of visitors with rewarding, satisfying and memorable experiences, solely depends on these individuals, whose personal att...

  9. THE FORMATION OF READERS IN THE ERA OF ITS “SACREDNESS”: IT WOULD BE THE SACRED THE CAUSE OF FASCINATION WITH CONTEMPORARY BESTSELLERS?

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    Jaime dos Reis Sant'Anna

    2016-07-01

    Full Text Available What explain the great appreciation by mass literature, as the bestseller Harry Potter or The Twilight Saga? We can use the mass literature to form readers in the school, or we must use only canonical literature? Finally, the more important discussion in this article: the sacred would be an enhancer element to explain the appreciation by these works and how would must the teachers debate this theme with their students?

  10. Assessment of traditional ecological knowledge and beliefs in the utilisation of important plant species: The case of Buhanga sacred forest, Rwanda

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    Runyambo Irakiza

    2016-07-01

    Full Text Available Traditional ecological knowledge is an integrated part of the African people and indeed the Rwandese for cultural purpose. Buhanga sacred forest is a relict forest of tremendous ecological importance to Rwandan society located in Musanze District. The aim of this study was to assess the traditional ecological knowledge and belief in the utilisation of some important plant species for the conservation of Buhanga sacred forest. Ecological information about ethnomedicinal and traditional practices were collected following structured questionnaire through interview involving eight traditional healers and three focus group discussions. Data were collected from the natural habitats, home gardens, farmlands and roadsides of Buhanga sacred forest. A total of 45 botanical taxa belonging to 28 families were reported to be used by the local community. Species such as Brillantaisia cicatricosa and Senna septemtrionalis were the popular species cited by traditional healers to treat human and animal diseases and ailments, respectively. The results of the study indicated that because of the cultural norms and values associated with the sacred forest, this has led to non-exploitation. The study presents key sites and plant species in which their use and belief can lead to their conservation. However, not only is it imperative to conserve traditional local knowledge for biocultural conservation motives but there is also need to train traditional healers on how to domesticate indigenous species as conservation measure because some species have become susceptible to extinction. Conservation implications: Highlighting indigenous species investigated in this research will provide a powerful tool for ensuring biodiversity conservation through community participation in a country of high population density in Africa. Some plant species that provided satisfactory Local Health Traditions among communities surrounding Buhanga can contribute as good material for further

  11. Is the Sacred ibis a real threat to biodiversity? Long-term study of its diet in non-native areas compared to native areas.

    Science.gov (United States)

    Marion, Loïc

    2013-04-01

    This paper presents the results of a 14-year study about the diet of the Sacred ibis in its main introduction area in France and its impact on native bird species, and compares the data to literature from its native area. During an initial period (1993-2004), the diet was essentially composed of invertebrates such as common aquatic insects (correlated with flooding) or Eristalis larvae picked from the mud (a vacant food niche in France), while scraps of meat taken from rubbish dumps were minor. These traditional preys taken from the same ecosystems as in its native area did not result in an exponential increase of the number of breeding Sacred ibises. Invasive Red swamp crayfish recently replaced other foods in its diet with a resulting sharp increase in breeding pair numbers (R(2)=0.48). As in other parts of the world, vertebrates constituted very accidental preys, and no bird species were really threatened by such predation. Conversely, the Sacred ibis can have a positive effect as a predator of invasive crayfish. Adding the species to the DAISIE list of the 100 most invasive alien species in Europe therefore appears debatable. Copyright © 2013 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  12. Sri Dalada Maligawa - 3D-Scanning and Documentation of the Temple of the Sacred Tooth Relic at Kandy, Sri Lanka

    Science.gov (United States)

    Rahrig, M.; Luib, A.

    2017-08-01

    Sri Dalada Maligawa - the Temple of the Sacred Tooth Relic - is one of the most important pilgrim sites in Buddhist culture. It is the main part of the UNESCO World Heritage Site Sacred City of Kandy. Since the end of the 17th century the temple has been keeping the sacred tooth of the Buddha. Until now an accurate documentation of the temple with all its rich decorations is missing. The Temple is built in an area vulnerable to environmental factors like earthquakes or monsoon rains and was the target of terrorist attacks. To help preserving this important cultural heritage a research project was carried out. Main part of the project was a 3D-documentation of the entire temple by using Terrestrial-Laser-Scanning (TLS) and the creating of CAD-Plans. In addition to the documentation of the architecture several details were taken in high resolution by Structured-Light-Scanning (SLS). All data will be part of the digital archive of the temple and were used as a base for a general site monitoring, especially to observe cracks. Next to the mere documentation a transfer of knowledge was another aim of the project. In future most of the analysis of the scan data can be done by local specialists.

  13. Sri Dalada Maligawa – 3D-Scanning and Documentation of the Temple of the Sacred Tooth Relic at Kandy, Sri Lanka

    Directory of Open Access Journals (Sweden)

    M. Rahrig

    2017-08-01

    Full Text Available Sri Dalada Maligawa – the Temple of the Sacred Tooth Relic – is one of the most important pilgrim sites in Buddhist culture. It is the main part of the UNESCO World Heritage Site Sacred City of Kandy. Since the end of the 17th century the temple has been keeping the sacred tooth of the Buddha. Until now an accurate documentation of the temple with all its rich decorations is missing. The Temple is built in an area vulnerable to environmental factors like earthquakes or monsoon rains and was the target of terrorist attacks. To help preserving this important cultural heritage a research project was carried out. Main part of the project was a 3D-documentation of the entire temple by using Terrestrial-Laser-Scanning (TLS and the creating of CAD-Plans. In addition to the documentation of the architecture several details were taken in high resolution by Structured-Light-Scanning (SLS. All data will be part of the digital archive of the temple and were used as a base for a general site monitoring, especially to observe cracks. Next to the mere documentation a transfer of knowledge was another aim of the project. In future most of the analysis of the scan data can be done by local specialists.

  14. Late Onset First Episode Psychosis Emerging as Delusional Misidentification of Familiar Sacred Places During a Holy Pilgrimage: A Case Report and Literature Review.

    Science.gov (United States)

    Awara, Mahmoud A; Moselhy, Hamdy F; Elnenaei, Manal O

    2017-11-07

    The delusional misidentification syndromes (DMS) include a myriad of discrete but related syndromes, which have wide spectrum anomalies of familiarity. Several misidentification syndromes have been described in the psychiatric literature, the most common of these delusions are: the Capgras syndrome; the Fregoli syndrome; the syndrome of inter-metamorphosis; reduplicative paramnesia; and environmental reduplication. The reported case highlights the emergence of late onset first episode psychosis in a Middle Eastern 65-year-old female who has no previous psychiatric history. The nature of psychosis was mainly delusions of misidentification and persecution. DMS are relatively rare and occur predominantly in association with schizophrenia and affective psychosis. Between 25 and 40% are associated with organic conditions such as dementia, head injuries, brain tumors, and epilepsy. Only three cases of misidentification of sacred places have been reported previously in the literature. This case report is the first to present a DMS, emerging as a late onset first episode psychosis during the sacred journey of Hajj. The reported case highlights the importance of early recognition and treatment of mental health conditions that may appear de novo during the Hajj sacred journey. Readily available psychiatric resources, psychotropic medications, and psycho-education may be pivotal in ensuring mental well-being of pilgrims, which is fundamental to maintain the mental capacity required for completing these journeys.

  15. “Native Skywatchers - Earth Sky Mirroring, Kapemni Pairs - Ojibwe and D(L)akota Sacred Star Sites “

    Science.gov (United States)

    Lee, Annette S.; Gawboy, Carl; Rock, Jim; Wilson, William; Tibbetts, Jeff; O'Rourke, Charleen

    2015-08-01

    Late in February, deep in the heart of the northern hemisphere winter, Ojibwe people know to look to the east a few hours after sunset and offer tobacco to Mizhi Bizhiw -Curly Tail, the Great Spirit Cat in the night sky. There are many beautiful and layered teachings about Mizhi Bizhiw - Curly Tail relating to the coming of spring, respect for the water, sugar bush camp, but most important; knowing the stars meant survival.Painted high on the granite rock cliffs above the glacial waters in red ochre is the Mizhi Bizhiw - Curly Tail constellation. Along with the Ojibwe lion are two neighboring seasonal constellations: Mooz (Pegasus) and Biboonkionini -Wintermaker (Orion+). On Lake Hegman in the Boundary Waters Canoe Area located near Ely, Minnesota, a sacred reflection, an earth-sky mirroring is illustrated. It is here that tangible and intangible star knowledge meet.In D(L)akota star knowledge one of the most important teachings is kapemni. Wrapped up in this one word are layers of meaning that can be thought of as ‘As it is above; it is below.’ Imagine two tipis stacked vertically. The top triangle is inverted so that the pair meets at the apex. It is understood that the top realm represents the sky above, the stars or the spirit world. The bottom tipi represents the Earth, the material or the physical world.On Summer Solstice each year the Sun can be found in the Mato Tipila - Bear’s Lodge D(L)akota constellation (Gemini). The day when this astronomical alignment happens is known as the ‘Wacipi - Sundance Ceremony in the Stars’. At this time traditionally D(L)akota people would meet at Mato Tipila Paha - Grey Horn Butte (Devil’s Tower) in northeast Wyoming and participate in the earthly Wacipi - Sun Dance ceremony. This is a beautiful example of a kapemni pair or an earth-sky mirroring.Presented here are just two examples of sacred star sites found in our region: Minnesota, North & South Dakota, US. These are examples of cultural heritage that is

  16. Evaporation-induced transition from Nepenthes pitcher-inspired slippery surfaces to lotus leaf-inspired superoleophobic surfaces.

    Science.gov (United States)

    Zhang, Junping; Wu, Lei; Li, Bucheng; Li, Lingxiao; Seeger, Stefan; Wang, Aiqin

    2014-12-02

    The newly developed Nepenthes pitcher (NP)-inspired slippery surfaces, formed by immobilizing fluoroliquids on lotus leaf (LL)-inspired superoleophobic surfaces, are of great general interest, whereas there are many interesting phenomena and fundamental scientific issues remaining to be unveiled. Here we present our findings of the effects of evaporation of the fluoroliquid, an inevitable process in most cases, -induced transition from NP-inspired to LL-inspired surfaces on the wettability, transparency, and self-cleaning property of the surfaces. The transition is controlled by regulating the evaporation temperature of the model fluoroliquid, Krytox100. The evaporation of Krytox100 has great a influence on the wettability, transparency, and self-cleaning property. An intermediate "sticky" state is observed in the transition process. We believe that our findings in the transition process are helpful in understanding the similarities and differences between the NP-inspired and LL-inspired surfaces and in designing new bioinspired antiwetting surfaces and exploring their potential applications.

  17. Presymbiotic factors released by the arbuscular mycorrhizal fungus Gigaspora margarita induce starch accumulation in Lotus japonicus roots.

    Science.gov (United States)

    Gutjahr, Caroline; Novero, Mara; Guether, Mike; Montanari, Ombretta; Udvardi, Michael; Bonfante, Paola

    2009-01-01

    * Nutrient exchange is the key symbiotic feature of arbuscular mycorrhiza (AM). As evidence is accumulating that plants sense presymbiotic factors from AM fungi and prepare for colonization, we investigated whether modifications in plant sugar metabolism might be part of the precolonization program. * Inoculation of Lotus japonicus roots in a double Millipore sandwich with the AM fungus Gigaspora margarita prevented contact between the symbionts but allowed exchange of signal molecules. Starch content was used as a marker for root carbohydrate status. * Mycorrhizal colonization of L. japonicus roots led to a decrease in starch concentration. In roots inoculated in the double sandwich, the polysaccharide accumulated after 1 wk and persisted for at least 4 wk. The response was absent in the castor myc(-) mutant, sym4-2, while transcript levels of both CASTOR and POLLUX were slightly enhanced in the wild-type L. japonicus roots, suggesting a requirement of the corresponding proteins for the starch-accumulation response. Exudates obtained from fungal spores germinated in the absence of the plant also induced starch accumulation in wild-type L. japonicus roots. * We conclude that factors released from germinating AM fungal spores induce changes in the root carbon status, possibly by enhancing sugar import, which leads to starch accumulation when colonization is prevented.

  18. Effects of salt stress on the expression of antioxidant genes and proteins in the model legume Lotus japonicus.

    Science.gov (United States)

    Rubio, Maria C; Bustos-Sanmamed, Pilar; Clemente, Maria R; Becana, Manuel

    2009-03-01

    Salt stress negatively affects many physiological processes in plants. Some of these effects may involve the oxidative damage of cellular components, which can be promoted by reactive oxygen species and prevented by antioxidants. The protective role of antioxidants was investigated in Lotus japonicus exposed to two salinization protocols: S1 (150 mM NaCl for 7 d) and S2 (50, 100 and 150 mM NaCl, each concentration for 6 d). Several markers of salt stress were measured and the expression of antioxidant genes was analyzed using quantitative reverse transcription–polymerase chain reaction and, in some cases, immunoblots and enzyme activity assays. Leaves of S1 plants suffered from mild osmotic stress, accumulated proline but noNa+, and showed induction of many superoxide dismutase and glutathione peroxidase genes. Leaves of S2 plants showed increases in Na+ and Ca2+, decreases in K+, and accumulation of proline and malondialdehyde. In leaves and roots of S1 and S2 plants, the mRNA, protein and activity levels of the ascorbate-glutathione enzymes remained constant, with a few exceptions. Notably, there was consistent up-regulation of the gene encoding cytosolic dehydroascorbate reductase, and this was possibly related to its role in ascorbate recycling in the apoplast. The overall results indicate that L. japonicus is more tolerant to salt stress than other legumes, which can be attributed to the capacity of the plant to prevent Na+reaching the shoot and to activate antioxidant defenses.

  19. Condensed tannins in the tissue culture of sainfoin (Onobrychis viciifolia Scop.) and birdsfoot trefoil (Lotus corniculatus L.).

    Science.gov (United States)

    Lees, G L

    1986-08-01

    Two forage legumes, birdsfoot trefoil (Lotus corniculatus L.) and sainfoin (Onobrychis viciifolia Scop.), containing condensed tannins in their leaves and stems were used as source material to study condensed tannins in tissue culture. More protoplasts were isolated from mesophyll tissue of a low tannin-containing strain of birdsfoot trefoil than from a high tannin-containing strain, but more tannin-filled protoplasts were observed in the latter. Growth rates of leaf explant-derived callus tissue were greater for the high-tannin than for the low-tannin strain. In sainfoin, callus cultures from leaf explants produced numerous tannin-filled cells by 21 days. Explants from sainfoin cotyledons and roots, tissues which normally do not contain tannins, also formed callus with tannin-filled cells in 21 days but in almost every case, a cytokinin was required for tannin formation to occur. The occurrence of tannin-filled cells in callus from root and cotyledon explants was variable and genotype specific. These results show that endogenous tannins can affect protoplast isolation and possibly callus growth in birds-foot trefoil, and that the formation of condensed tannins in sainfoin callus culture can be influenced by a growth regulator.

  20. Lotus japonicus flowers are defended by a cyanogenic β-glucosidase with highly restricted expression to essential reproductive organs.

    Science.gov (United States)

    Lai, Daniela; Pičmanová, Martina; Abou Hachem, Maher; Motawia, Mohammed Saddik; Olsen, Carl Erik; Møller, Birger Lindberg; Rook, Fred; Takos, Adam M

    2015-09-01

    Flowers and leaves of Lotus japonicus contain α-, β-, and γ-hydroxynitrile glucoside (HNG) defense compounds, which are bioactivated by β-glucosidase enzymes (BGDs). The α-HNGs are referred to as cyanogenic glucosides because their hydrolysis upon tissue disruption leads to release of toxic hydrogen cyanide gas, which can deter herbivore feeding. BGD2 and BGD4 are HNG metabolizing BGD enzymes expressed in leaves. Only BGD2 is able to hydrolyse the α-HNGs. Loss of function mutants of BGD2 are acyanogenic in leaves but fully retain cyanogenesis in flowers pointing to the existence of an alternative cyanogenic BGD in flowers. This enzyme, named BGD3, is identified and characterized in this study. Whereas all floral tissues contain α-HNGs, only those tissues in which BGD3 is expressed, the keel and the enclosed reproductive organs, are cyanogenic. Biochemical analysis, active site architecture molecular modelling, and the observation that L. japonicus accessions lacking cyanogenic flowers contain a non-functional BGD3 gene, all support the key role of BGD3 in floral cyanogenesis. The nectar of L. japonicus flowers was also found to contain HNGs and additionally their diglycosides. The observed specialisation in HNG based defence in L. japonicus flowers is discussed in the context of balancing the attraction of pollinators with the protection of reproductive structures against herbivores.

  1. Lotus corniculatus Crop Growth in Crude Oil Polluted Soil. Part1 Total Petroleum Hydrocarbons Reduction of Polluted and Cultivated Soil

    Directory of Open Access Journals (Sweden)

    Smaranda Masu

    2016-05-01

    Full Text Available The use of power plant fly ash, by its physical-chemical properties, can significantly change the characteristics of soils polluted with oil (Total Petroleum Hydrocarbons, TPH, in their rehabilitation process, if combined with biodegradable organic materials, wastes such as sewage sludge from municipal wastewater treatment plant. Maintaining vegetation on soils polluted with 80.5 ± 3.9 g·kg-1 D.M. of TPH under perennial regime specific to bird’s foot trefoil (Lotus corniculatus, demonstrates the tolerance of the plant to the created conditions by the treatment of polluted soil with adequate amounts of fertilizer and fly ash from burning coal in power stations. The addition of 50-500 g fly ash per vegetation pot equipped with crude oil polluted soil mixed with 250 g sewage sludge per pot has reduced the oil content in the soil, in two ways: on the one hand influenced by the state of development of plants and on the other hand by weather conditions (alternation of seasons. The amount of TPH lost during the 16 months of vegetation in soils polluted with 80.5±3.9 g·kg-1 D.M. was 73.3-77.5 g·kg-1 D.M.

  2. Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity.

    Science.gov (United States)

    Mkadmini Hammi, Khaoula; Hammami, Majdi; Rihouey, Christophe; Le Cerf, Didier; Ksouri, Riadh; Majdoub, Hatem

    2016-12-01

    Response surface methodology using a Box-Behnken design was employed to optimize extraction temperature, extraction time and ratio of water to material to obtain a maximum polysaccharide yield with high uronic acid content and antioxidant property from edible Zizyphus lotus fruit. The optimal conditions were: extraction time of 3h 15min, extraction temperature of 91.2°C and water to solid ratio of 39mL/g. Under these conditions, the experimental extraction yield, uronic acid content and 2,2-diphenyl-1-picrylhydrazyl scavenging ability (IC50) were 18.88%, 41.89 and 0.518mg/mL, respectively. Chemical analysis revealed that the extract was composed of 97.92% carbohydrate of which 41.89% is uronic acid. The extracted polysaccharides, with an average molecular weight of 2720kDa, are composed of arabinose, rhamnose, glucose, fructose, galactose and xylose. Moreover, the polysaccharides exhibited a significant reducing power and anti-lipid peroxidation activities. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Optimization of ultrasound-assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology.

    Science.gov (United States)

    Hammi, Khaoula Mkadmini; Jdey, Ahmed; Abdelly, Chedly; Majdoub, Hatem; Ksouri, Riadh

    2015-10-01

    The optimization of antioxidant extraction conditions from a ripe edible fruits of Zizyphus lotus (L.) with an ultrasound-assisted system was achieved by response surface methodology. The central composite rotatable design was employed for optimization of extraction parameters in terms of total phenolic content and antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and phosphomolybdenum assay. The optimum operating conditions for extraction were as follows: ethanol concentration, 50%; extraction time, 25 min; extraction temperature, 63°C and ratio of solvent to solid, 67 mL/g. Under these conditions, the obtained extract exhibited a high content of phenolic compounds (40.782 mg gallic acid equivalents/g dry matter) with significant antioxidant properties (the total antioxidant activity was 75.981 mg gallic acid equivalents/g dry matter and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was 0.289 mg/mL). Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Deep sequencing of Lotus corniculatus L. reveals key enzymes and potential transcription factors related to the flavonoid biosynthesis pathway.

    Science.gov (United States)

    Wang, Ying; Hua, Wenping; Wang, Jian; Hannoufa, Abdelali; Xu, Ziqin; Wang, Zhezhi

    2013-04-01

    Lotus corniculatus L. is used worldwide as a forage crop due to its abundance of secondary metabolites and its ability to grow in severe environments. Although the entire genome of L. corniculatus var. japonicus R. is being sequenced, the differences in morphology and production of secondary metabolites between these two related species have led us to investigate this variability at the genetic level, in particular the differences in flavonoid biosynthesis. Our goal is to use the resulting information to develop more valuable forage crops and medicinal materials. Here, we conducted Illumina/Solexa sequencing to profile the transcriptome of L. corniculatus. We produced 26,492,952 short reads that corresponded to 2.38 gigabytes of total nucleotides. These reads were then assembled into 45,698 unigenes, of which a large number associated with secondary metabolism were annotated. In addition, we identified 2,998 unigenes based on homology with L. japonicus transcription factors (TFs) and grouped them into 55 families. Meanwhile, a comparison of four tag-based digital gene expression libraries, built from the flowers, pods, leaves, and roots, revealed distinct patterns of spatial expression of candidate unigenes in flavonoid biosynthesis. Based on these results, we identified many key enzymes from L. corniculatus which were different from reference genes of L. japonicus, and five TFs that are potential enhancers in flavonoid biosynthesis. Our results provide initial genetics resources that will be valuable in efforts to manipulate the flavonoid metabolic pathway in plants.

  5. Model systems with extreme aspect ratio, tunable geometry, and surface functionality for a quantitative investigation of the Lotus effect.

    Science.gov (United States)

    Hassel, Achim Walter; Milenkovic, Srdjan; Schürmann, Ulrich; Greve, Henry; Zaporojtchenko, Vladimir; Adelung, Rainer; Faupel, Franz

    2007-02-13

    Superhydrophobicity and superhydrophilicity of surfaces are key properties for fabrication of self-cleaning surfaces (Lotus effect). It is well known that the mechanism behind this is based on the surface roughness and surface functionalization. To obtain an understanding of the details of the underlying mechanism, a metal system based on a eutectic is suggested. In this study, a wide range tunability of its needlelike narrow size distributed nanostructure is demonstrated. The length of the needles as well as their density can be varied independently. In addition, an important parameter for the wettability, the roughness, is related directly to the growth parameters, which lead to excellent controllable and reproducible eutectic structures. Simply by varying etching time very high aspect ratios can be achieved, allowing studying the interaction of the very long needles with liquids. Moreover, the surface functionality can be tuned by RF-magnetron sputtering of PTFE onto the metal needles. As those layers can be very thin, our system allows, in principle, studying the transition from a metal to a polymer surface using submonolayers. Furthermore, the first contact angle measurements on the nanostructured and functionalized eutectic structures are presented and discussed.

  6. Facile Adhesion-Tuning of Superhydrophobic Surfaces between "Lotus" and "Petal" Effect and Their Influence on Icing and Deicing Properties.

    Science.gov (United States)

    Nine, Md J; Tung, Tran Thanh; Alotaibi, Faisal; Tran, Diana N H; Losic, Dusan

    2017-03-08

    Adhesion behavior of superhydrophobic (SH) surfaces is an active research field related to various engineering applications in controlled microdroplet transportation, self-cleaning, deicing, biochemical separation, tissue engineering, and water harvesting. Herein, we report a facile approach to control droplet adhesion, bouncing and rolling on properties of SH surfaces by tuning their air-gap and roughness-height by altering the concentrations of poly dimethyl-siloxane (PDMS). The optimal use of PDMS (4-16 wt %) in a dual-scale (nano- and microparticles) composite enables control of the specific surface area (SSA), pore volume, and roughness of matrices that result in a well-controlled adhesion between water droplets and SH surfaces. The sliding angles of these surfaces were tuned to be varied between 2 ± 1 and 87 ± 2°, which are attributed to the transformation of the contact type between droplet and surface from "point contact" to "area contact". We further explored the effectiveness of these low and high adhesive SH surfaces in icing and deicing actions, which provides a new insight into design highly efficient and low-cost ice-release surface for cold temperature applications. Low adhesion (lotus effect) surface with higher pore-volume exhibited relatively excellent ice-release properties with significant icing delay ability principally attributed to the large air gap in the coating matrix than SH matrix with high adhesion (petal effect).

  7. Photoinduced self-epitaxial crystal growth of a diarylethene derivative with antireflection moth-eye and superhydrophobic lotus effects.

    Science.gov (United States)

    Nishikawa, Naoki; Sakiyama, Shingo; Yamazoe, Seiji; Kojima, Yuko; Nishihara, Ei-ichiro; Tsujioka, Tsuyoshi; Mayama, Hiroyuki; Yokojima, Satoshi; Nakamura, Shinichiro; Uchida, Kingo

    2013-06-25

    We identified the mechanism of the formation of needle-shaped microcrystals on which the contact angle of a water droplet exceeds 170° [Nishikawa, N. et al. Langmuir, 2012, 28, 17817-17824]. The standing needle-shaped crystal of the closed-ring isomer of a diarylethene 3c grew at a much lower temperature than the eutectic temperature by irradiation of UV light on the thin films of the open-ring isomer 3o, due to the epitaxial growth of the 013 plane of 3c over the 110 plane of the crystal lattice of 3o in the subphase. Therefore, the new crystal-growth mechanism triggered by the photoisomerization does not require special inorganic single-crystal substrates and may be called self-epitaxial crystal growth. The needle-shaped crystals appeared well-ordered and stood inclined at an angle of about 60° to the surface. Consequently, the photo-induced rough surface shows not only the superhydrophobic lotus effect, but also the antireflection moth-eye effect, and these effects were switchable by alternate irradiation with UV and visible light.

  8. Identification and functional characterization of a sulfate transporter induced by both sulfur starvation and mycorrhiza formation in Lotus japonicus.

    Science.gov (United States)

    Giovannetti, Marco; Tolosano, Matteo; Volpe, Veronica; Kopriva, Stanislav; Bonfante, Paola

    2014-11-01

    Arbuscular mycorrhizas (AMs) are one of the most widespread symbioses in the world. They allow plants to receive mineral nutrients from the symbiotic fungus which in turn gets back up to 20% of plant carbon and completes its life cycle. Especially in low-nutrient conditions, AM fungi are capable of significantly improving plant phosphate and nitrogen acquisition, but fewer data are available about sulfur (S) nutrition. We focused on S metabolism in Lotus japonicus upon mycorrhizal colonization under sulfur starvation or repletion. We investigated both tissue sulfate concentrations and S-related gene expression, at cell-type or whole-organ level. Gene expression and sulfate tissue concentration showed that Rhizophagus irregularis colonization can improve plant S nutritional status under S starvation. A group 1 sulfate transporter, LjSultr1;2, induced by both S starvation and mycorrhiza formation, was identified. Its transcript was localized in arbuscule-containing cells, which was confirmed with a promoter-GUS assay, and its function was verified through phenotyping of TILLING mutants in nonmycorrhizal seedlings. LjSultr1;2 thus appears to encode a key protein involved in plant sulfate uptake. In contrast to phosphate transporters, a single gene, LjSultr1;2, seems to mediate both direct and symbiotic pathways of S uptake in L. japonicus. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

  9. Date stones of Phoenix dactylifera and jujube shells of Ziziphus lotus as potential biosorbents for anionic dye removal.

    Science.gov (United States)

    El Messaoudi, Noureddine; Dbik, Abdellah; El Khomri, Mohammed; Sabour, Abderrahman; Bentahar, Safae; Lacherai, Abdellah

    2017-11-02

    This study investigated the biosorption of Congo Red (CR) from aqueous solution by Date stones (DS) of Phoenix dactylifera and jujube shells (JS) of Ziziphus lotus. Batch operations were carried out in the liquid phase to observe the effect of various experimental parameters such as contact time, pH, temperature, and initial dye concentration on removal of CR. The characteristics of the DS and JS were also examined by Fourier transform infrared analysis. The biosorption data of CR on DS and JS samples were well fitted with the Langmuir isotherm and pseudo-second kinetics model with a maximum biosorption amount of 45.08 mg g -1 for DS and 59.55 mg g -1 for JS at pH = 4, temperature of 50°C, and an initial concentration of 100-800 mg L -1 after 90 minutes of contact time. The outcomes indicated that DS and JS can be used as a good low-cost alternative for the treatment of effluents containing CR in water.

  10. Without a voice, with a violated body: Re-reading Judges 19 to challenge gender violence in sacred texts

    Directory of Open Access Journals (Sweden)

    Masenya, Madipoane J. (ngwan’a Mphahlele

    2012-06-01

    Full Text Available Although they are a majority of the South African population, African women in South Africa remain on the periphery of the margins of our communities. They are women who, although are a majority, mostly remain without a voice. Does it occasion any surprise then that they continue to be the face of violence in our contexts? It is a fact that the present South African landscape is characterised by, among other social evils, the violent acts perpetrated against women and children. That South Africa ranks among the leading countries in the world with appalling statistics on violence against women is well known. Such violence against a section of the South African population is entrenched by, among others, pronounced patriarchies, female voicelessness, dangerous masculinities and violent biblical hermeneutics. The latter hermeneutics is buttressed by some violent sacred texts interpreted in our predominantly patriarchal contexts. The present article seeks, among others, to bring a voice to the muted voice of the pilegesh in the text of Judges 19 by challenging gender-based violence both in the biblical text and in the African-South African context.

  11. Factors Contributing to the Decline of Traditional Practices in Communities from the Gwallek-Kedar area, Kailash Sacred Landscape, Nepal.

    Science.gov (United States)

    Atreya, Kishor; Pyakurel, Dipesh; Thagunna, Krishna Singh; Bhatta, Laxmi Dutt; Uprety, Yadav; Chaudhary, Ram Prasad; Oli, Bishwa Nath; Rimal, Sagar Kumar

    2018-02-27

    Traditional knowledge and practices are increasingly recognized in the resource conservation and management practices, however are declining in many parts of the world including Nepal. Studies on the inventory of traditional knowledge are available, albeit limited, and empirical analysis of factors contributing to the decline of traditional knowledge are negligible in Nepal. We thus initiated this study in the Nepal part of the Kailash Sacred Landscape to (i) document traditional knowledge and practices on agriculture, forest-based herbal remedy, and genetic resource conservation; and (ii) identify factors contributing to the decline of traditional practices in the communities. Data was collected during September-December 2015 through key informant interviews, focus group discussions, and households survey. The household survey data was used in binary logistic regression analysis to identify factors contributing to the decline of six key traditional practices. The study documented 56 types of traditional practices. The regressions showed that the age of the respondent, distance to the nearest forest, distance to the nearest motorable road, family members' ill health, and seasonal migration of the household members for jobs significantly influencing to the decline of the particular traditional practices, however, their effects vary within a practice and among the practices. The use of modern medicine, increasing road linkages, decreasing trend of plant resource availability, and agriculture intensification are responsible for the decline of the particular traditional practices. We recommend to recognize their significance in the governing socio-ecological systems and to link the traditional and scientific knowledge systems through policy formulations.

  12. Sacred Land –Roman Empire – Byzantium — Rus’: The Concept of Heredity in Old Russian Literature

    Directory of Open Access Journals (Sweden)

    Vladimir M. Kirillin

    2018-03-01

    Full Text Available The article examines the history of the idea of connection, succession, and hereditybetween Old Rus’, on the one hand, and such ancient political and spiritual centers ofthe Christian world as Jerusalem, Rome, and Constantinople on the other, within theOld Russian political thought. This idea was never documented in particular treatisesbut was nonetheless present in fictional, polemical and didactic works whether as amarginal or central theme, whether directly or allegorically. The author of the article considerably extends the circle of sources that drew retrospective analogies between Russian history and Sacred Land history. Indirect parallels in the “Word about the Law and the Grace” become explicit and specific in the hagiographic legend about Kiev Prince Vladimir Svyatoslavich and later recur, with different accents, in a number of literary texts in the 15 th –17 th centuries, reflecting the course of Russian history. In these texts, we encounter a division between Rome as a symbol of imperial mundane power associated with state politics, and Jerusalem as a symbol of the Kingdom of God associated with church and religion.

  13. [Comment on “Science and Sociology Butt Heads in Tomography Experiment in Sacred Mountains”] Learning Lessons From Science Experiments

    Science.gov (United States)

    Hollister, Lincoln S.

    I am writing to comment on the article by Baldridge et al., “Science and Sociology Butt Heads in Tomography Experiment in Sacred Mountains” (Eos, September 30,1997). I strongly support their conclusion that scientists must learn to communicate with the public if we are to continue to receive taxpayer support for our projects.I am lead principal investigator of the interdisciplinary project ACCRETE (funded by the Continental Dynamics program of NSF), which also involved what could easily have been perceived to be an environmentally and culturally invasive seismic experiment. We proposed to take the 240-foot seismic ship R/V Maurice Ewing through the inland waterways of southeast Alaska and British Columbia, firing airguns every 20 seconds for 10 days, all within “earshot” of marine mammals and fish. Consider the permitting challenge involved with two countries engaged in a fish war and three ethnically distinct First Nations who are in the midst of land claims negotiations. In addition, we had to deal with commercial fishing interests (imagine sailing a ship with 4 km of towed instruments through a couple hundred fishing boats with their nets out), marine mammal protection groups, a wilderness area, and a nuclear submarine base. We had many of the experiences Baldridge et al. report, but nevertheless were able to do our experiment as planned, without any modification imposed by local groups.

  14. EELS data acquisition, processing and display for the Zeiss CEM 902 based on LOTUS 1-2-3: application examples from a biological system and inorganic transition metal compounds.

    Science.gov (United States)

    Drechsler, M; Cantow, H J

    1991-04-01

    A personal computer combined with LOTUS 1-2-3 software, including the RS232 module of LOTUS MEASURE and a 12-bit ADC, has been used for data acquisition of electron energy-loss spectroscopy (EELS) spectra with the Zeiss CEM 902. The internal macro language of LOTUS 1-2-3 allows a menu-driven procedure. Macro-programs partly combined with external FORTRAN programs can be chosen from the menu for background subtraction, removal of multiple scattering effects by deconvolution, elemental quantification and several utilities. For special applications or conditions the macro programs can easily be modified. Spectra from crystals of two inorganic transition metal compounds, ruthenium trichloride and vanadium disulphide, and from a biological sample are presented as examples of the application of this software.

  15. Disponibilidade e valor nutritivo de forragem de leguminosas nativas (Adesmia DC. e exóticas (Lotus L. Availability and nutritive value of the wild leguminous (Adesmia DC. and exotics (Lotus L.

    Directory of Open Access Journals (Sweden)

    Simone Meredith Scheffer-Basso

    2001-06-01

    Full Text Available O gênero Adesmia DC. possui 17 espécies nativas no Brasil, distribuídas nos Estados do Sul, cuja importância está vinculada a sua adaptação ao solo e clima regionais, além de ser de crescimento hibernal (temperadas. Este trabalho teve o objetivo comparar o padrão de acúmulo de matéria seca (MS e valor nutritivo de forragem de A. latifolia, A. punctata e A. tristis, tendo como padrão Lotus corniculatus (cornichão e L. uliginosus. O ensaio foi realizado em casa de vegetação durante 210 dias (4000 graus-dia. A disponibilidade de forragem (DF foi similar entre A. latifolia (276 g MS/m² e cornichão (275 g MS/m² e entre A. tristis (201g MS/m² e L. uliginosus (192 g MS/m², sendo que A. punctata apresentou a menor DF (155 g MS/m². A. latifolia caracterizou-se pela maior precocidade na DF, devido ao crescimento mais rápido em relação às demais espécies, sugerindo seu potencial para utilização durante a estação fria. Em relação às análises de qualidade, o teor de proteína bruta (PB nas folhas de A. latifolia foi de até 21,6% e a DIVMO atingiu 72,3%. Os maiores conteúdos de PB e DIVMO foram encontrados nas folhas de cornichão, 30,3 e 75,8%, respectivamente. A. tristis apresentou DIVMO muito baixa nos caules (34,9 a 44,7%, o que poderia limitar seu consumo por bovinos. Concluiu-se que, entre as espécies de Adesmia estudadas, A. latifolia detém o maior potencial forrageiro, sugerindo a continuidade de estudos com a espécie.The genus Adesmia DC. has 17 species native to Brazil, distributed in the Southern states, whose importance is linked to its adaptation to the soils and climatic conditions of the region, besides being an active winter-growing species (temperate. This work aimed to compare the patterns of dry matter (DM accumulation and nutritive value of A. latifolia, A. punctata and A. tristis, using Lotus corniculatus (birdsfoot trefoil and L. uliginosus (big trefoil, as checks. The experiment was carried out in

  16. Quantification of total phenols, flavonoides and tannins from Ziziphus jujuba (mill. and Ziziphus lotus (l. (Desf. Leaf extracts and their effects on antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Meriem Elaloui

    2017-01-01

    Full Text Available This work was performed to determine the biochemical composition, antioxidant and antimicrobial activities of leaf extracts collected from four different provenances: Mahdia and Mahres (Ziziphus jujuba; Kairouan and Rouhia (Ziziphus lotus. Total phenols, flavonoids, tannins contents and antioxidant activity were evaluated using the Folin ciocalteux, Aluminum trichloride, vanillin and scavenging activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH radicals methods, respectively. The antimicrobial activity was evaluated against three bacterial strains (Escherichia coli, Staphylococcus aureus and klebsiella pneumoniea and three fungal strains (Fusarium culmorum, Fusarium solani and Botrytis cinerea, according to well Agar diffusion method. Total phenols and flavonoids were present at levels of 21.98 mg GAE /g DW and 7.80 mg ER/g DW; respectively in Ziziphus lotus. These levels did not exceeded 13.70 mg GAE /g DW and 6.73 mg ER/g DW for Ziziphus jujuba. The tannin contents were present in equal levels (7.9 mg EC/g DW in two species. The high antioxidant activity (0.01 µg/ml was noted in Rouhia provenance. The Ziziphus lotus leaf extracts showed promising efficiency against all tested microorganisms with a zone of inhibition ranging between 22 and 23.5 mm. This study could validate the medicinal potential of Ziziphus specie and explain why tunisian people traditionally use it in medicine to treat several pathologies. Ziziphus leaf extracts may be suggested in foods and pharmaceutical industries. Leaf extracts proved also to be effective against tested microorganisms. So, an adequate toxicological study must be carried out to verify the possibility of using these plants for fighting microorganisms.

  17. Genetic Diversity and Host Range of Rhizobia Nodulating Lotus tenuis in Typical Soils of the Salado River Basin (Argentina)▿ †

    Science.gov (United States)

    Estrella, María Julia; Muñoz, Socorro; Soto, María José; Ruiz, Oscar; Sanjuán, Juan

    2009-01-01

    A total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating Lotus tenuis in typical soils of the Salado River Basin. A high level of genetic diversity was revealed by repetitive extragenic palindromic PCR, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters A and B, after 16S rRNA restriction fragment length polymorphism analysis. Cluster A strains appeared to be related to the genus Mesorhizobium, whereas cluster B was related to the genus Rhizobium. 16S rRNA sequence and phylogenetic analysis further supported the distribution of most of the symbiotic isolates in either Rhizobium or Mesorhizobium: the only exception was isolate BA135, whose 16S rRNA gene was closely related to the 16S rRNA gene of the genus Aminobacter. Most Mesorhizobium-like isolates were closely related to Mesorhizobium amorphae, Mesorhizobium mediterraneum, Mesorhizobium tianshanense, or the broad-host-range strain NZP2037, but surprisingly few isolates grouped with Mesorhizobium loti type strain NZP2213. Rhizobium-like strains were related to Rhizobium gallicum, Rhizobium etli, or Rhizobium tropici, for which Phaseolus vulgaris is a common host. However, no nodC or nifH genes could be amplified from the L. tenuis isolates, suggesting that they have rather divergent symbiosis genes. In contrast, nodC genes from the Mesorhizobium and Aminobacter strains were closely related to nodC genes from narrow-host-range M. loti strains. Likewise, nifH gene sequences were very highly conserved among the Argentinian isolates and reference Lotus rhizobia. The high levels of conservation of the nodC and nifH genes suggest that there was a common origin of the symbiosis genes in narrow-host-range Lotus symbionts, supporting the hypothesis that both intrageneric horizontal gene transfer and intergeneric horizontal gene transfer are important mechanisms for the spread of symbiotic capacity in the Salado River Basin. PMID

  18. CLE peptide-encoding gene families in Medicago truncatula and Lotus japonicus, compared with those of soybean, common bean and Arabidopsis

    DEFF Research Database (Denmark)

    Hastwell, April H; de Bang, Thomas Christian; Gresshoff, Peter M

    2017-01-01

    these complete CLE peptide-encoding gene families with those of fellow legumes, Glycine max and Phaseolus vulgaris, in addition to the model plant Arabidopsis thaliana. This approach provided insight into the evolution of CLE peptide families and enabled us to establish putative M. truncatula and L. japonicus...... in controlling legume nodulation. Here, the entire family of CLE peptide-encoding genes was identified in Medicago truncatula (52) and Lotus japonicus (53), including pseudogenes and non-functional sequences that were identified. An array of bioinformatic techniques were used to compare and contrast...

  19. Lotus japonicus nodulation requires two GRAS domain regulators, one of which is functionally conserved in a non-legume

    DEFF Research Database (Denmark)

    Heckmann, Anne Birgitte Lau; Lombardo, Fabien; Miwa, Hiroki

    2006-01-01

    A new nodulation-defective mutant of Lotus japonicus does not initiate nodule cortical cell division in response to Mesorhizobium loti, but induces root hair deformation, Nod factor-induced calcium spiking, and mycorrhization. This phenotype, together with mapping data, suggested that the mutation...... could be in the ortholog of the Medicago truncatula NSP1 gene (MtNSP1). The sequence of the orthologous gene (LjNSP1) in the L. japonicus mutant (Ljnsp1-1) revealed a mutation causing a premature stop resulting in loss of the C-terminal 23 amino acids. We also sequenced the NSP2 gene from L. japonicus...

  20. Lotus japonicus nodulation requires two GRAS-domain regulators, NSP1 and NSP2

    DEFF Research Database (Denmark)

    Heckmann, Anne Birgitte Lau; Lombardo, Fabien; Miwa, Hiroki

    A nodulation-defective mutant of Lotus japonicus does not initiate nodule cortical cell division in response to Mesorhizobium loti, but induces root hair deformation, Nod factor-induced calcium spiking, and mycorrhization. We have shown that this mutant has a premature stop in the NSP1 (Nodulation...... Signalling Pathway 1) gene (Ljnsp1-1) resulting in loss of the C-terminal 23 amino acids (aa) and we recently identified another mutant in NSP1 (Ljnsp1-2) with a truncated protein of 341 aa. Additionally, we have sequenced and isolated a mutant in LjNSP2 (Ljnsp2-3) that has a premature stop codon showing...

  1. Lotus-like effect for metal filings recovery and particle removal on heated metal surfaces using Leidenfrost water droplets.

    Science.gov (United States)

    Tan, Cher Lin Clara; Sapiha, Kostantyn; Leong, Yoke Fun Hannah; Choi, Siwon; Anariba, Franklin; Thio, Beng Joo Reginald

    2015-07-21

    A "lotus-like" effect is applied to demonstrate the ability of the Leidenfrost water droplets to recover Cu particles on a heated Al substrate. Cu particles on the heated surface adhere to the rim of the Leidenfrost droplets and eventually coat the droplets' surface to form an aggregation. When Fe filings are added to the Cu particles, the aggregated mixture can then be collected using a strong rare earth magnet (NdFeB) upon evaporation of the water. We also show that the Leidenfrost effect can be effectively utilized to recover both hydrophobic (dust and activated carbon) and hydrophilic (SiO2 and MgO) particles from heated Al surfaces without any topographical modification or surfactant addition. Our results show that hydrophobic and hydrophilic materials can be collected with >92% and >96% effectiveness on grooved and smooth Al surfaces, respectively. Furthermore, we observed no significant differences in the amount of material collected above the Leidenfrost point within the tested temperature range (240 °C vs. 340 °C) as well as when the Al sheet was replaced with a Cu sheet as the substrate. However, we did observe that the Leidenfrost droplets were able to collect a greater amount of material when the working liquid was water than when it was ethanol. Our findings show promise in the development of an effective precious coinage metal filings recovery technology for application in the mint industry, as well as the self-cleaning of metallic and semiconductor surfaces where manual cleaning is not amenable.

  2. The mixture of procyanidins extracted from the lotus seed pod and bilobalide ameliorates scopolamine-induced memory impairment in mice.

    Science.gov (United States)

    Zhang, Li; Xu, Ji-Qu; Rong, Shuang; Xie, Bi-Jun; Sun, Zhi-Da; Zhang, Yun-Jian; Liu, Lie-Gang

    2009-08-01

    To study the co-effect of procyanidins extracted from the lotus seed pod (LSPC) and bilobalide (BIL) on ameliorating scopolamine-induced learning and memory impairment in young mice. Fifty male Kunming mice with similar learning and memory capabilities were selected by Morris water maze test and were randomized into 5 groups (n=10 in each group): control group, scopolamine group, L-(LSPC+BIL) group (50 mg/kg LSPC+10 mg/kg BIL), M-(LSPC+BIL) group (100 mg/kg LSPC+20 mg/kg BIL), H-(LSPC+BIL) group (150 mg/kg LSPC+30 mg/kg BIL). Scopolamine model with impaired learning and memory was established by scopolamine treatment (1 mg/kg), and after 10 min mice were tested. In L-, M-, and H-(LSPC+BIL) groups, mice were treated with LSPC and BIL ig. for 30 days, while mice in the other 2 groups were treated with normal saline ig. instead. After the 30-day's treatment, the co-effect of LSPC and BIL on learning and memory was tested by Morris water maze and the step-down avoidance tests. The memory impairment caused by scopolamine in young mice could be ameliorated by co-treatment of LSPC and BIL, as indicated by significantly shorter escape latency and swimming distance in the Morris water maze test, when compared with those in the scopolamine group. In the step-down avoidance test, mice in all the 3 dose groups showed significantly smaller number of errors and longer latency than mice in the scopolamine group did. Co-treatment of LSPE and BIL can ameliorate scopolamine-induced learning and memory impairment in young mice.

  3. Ameliorative effect of lotus seedpod proanthocyanidins on cognitive impairment and brain aging induced by D-galactose.

    Science.gov (United States)

    Gong, Yu-Shi; Guo, Juan; Hu, Kun; Gao, Yong-Qing; Xie, Bi-Jun; Sun, Zhi-Da; Yang, Er-Ning; Hou, Fang-Li

    2016-02-01

    This study mainly investigated the ameliorative effect of lotus seedpod proanthocyanidins (LSPC) and the mechanism underlying such effect on cognitive impairment and brain aging induced by d-galactose. Aging mice induced by d-galactose (150 mg/kg, sc injection daily for 6 weeks) were chosen for the experiment. LSPCs (30, 60, and 90 mg/kg, ig) were provided after d-galactose injection. Learning and memory functions were detected by Y-maze and step-down avoidance tests. Then, some biochemical indexes related to cognitive ability and aging were measured. Histopathological feature and P53 protein expression in the hippocampus were observed. Results showed that the three different doses of LSPC could significantly ameliorate the learning and memory abilities impaired by d-galactose. LSPC significantly reduced the levels of malondialdehyde and nitric oxide (i.e. 90 mg/kg LSPC group vs. model group, P=0.008), reduced the content of β-amyloid peptide 1-42 (i.e. 90 mg/kg LSPC group vs. model group, P=0.009), decreased the activities of acetylcholinesterase, monoamine oxidase B, total nitric oxide synthase (i.e. 90 mg/kg LSPC group vs. model group, P=0.006), and neuronal nitric oxide synthase and synchronously increased the activities of superoxide dismutase and glutathione peroxidase in the brain. Furthermore, LSPC could prevent neuron damage and could lessen the expression of P53 protein in the hippocampus. These findings demonstrated that LSPC effectively attenuated cognitive damage and improved parameters related to brain aging in senescent mice induced by d-galactose, and may be used to treat Alzheimer's disease. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. Gibberellins interfere with symbiosis signaling and gene expression and alter colonization by arbuscular mycorrhizal fungi in Lotus japonicus.

    Science.gov (United States)

    Takeda, Naoya; Handa, Yoshihiro; Tsuzuki, Syusaku; Kojima, Mikiko; Sakakibara, Hitoshi; Kawaguchi, Masayoshi

    2015-02-01

    Arbuscular mycorrhiza is a mutualistic plant-fungus interaction that confers great advantages for plant growth. Arbuscular mycorrhizal (AM) fungi enter the host root and form symbiotic structures that facilitate nutrient supplies between the symbionts. The gibberellins (GAs) are phytohormones known to inhibit AM fungal infection. However, our transcriptome analysis and phytohormone quantification revealed GA accumulation in the roots of Lotus japonicus infected with AM fungi, suggesting that de novo GA synthesis plays a role in arbuscular mycorrhiza development. We found pleiotropic effects of GAs on the AM fungal infection. In particular, the morphology of AM fungal colonization was drastically altered by the status of GA signaling in the host root. Exogenous GA treatment inhibited AM hyphal entry into the host root and suppressed the expression of Reduced Arbuscular Mycorrhization1 (RAM1) and RAM2 homologs that function in hyphal entry and arbuscule formation. On the other hand, inhibition of GA biosynthesis or suppression of GA signaling also affected arbuscular mycorrhiza development in the host root. Low-GA conditions suppressed arbuscular mycorrhiza-induced subtilisin-like serine protease1 (SbtM1) expression that is required for AM fungal colonization and reduced hyphal branching in the host root. The reduced hyphal branching and SbtM1 expression caused by the inhibition of GA biosynthesis were recovered by GA treatment, supporting the theory that insufficient GA signaling causes the inhibitory effects on arbuscular mycorrhiza development. Most studies have focused on the negative role of GA signaling, whereas our study demonstrates that GA signaling also positively interacts with symbiotic responses and promotes AM colonization of the host root. © 2015 American Society of Plant Biologists. All Rights Reserved.

  5. Mimicking both petal and lotus effects on a single silicon substrate by tuning the wettability of nanostructured surfaces.

    Science.gov (United States)

    Dawood, M K; Zheng, H; Liew, T H; Leong, K C; Foo, Y L; Rajagopalan, R; Khan, S A; Choi, W K

    2011-04-05

    We describe a new method of fabricating large-area, highly scalable, "hybrid" superhydrophobic surfaces on silicon (Si) substrates with tunable, spatially selective adhesion behavior by controlling the morphologies of Si nanowire arrays. Gold (Au) nanoparticles were deposited on Si by glancing-angle deposition, followed by metal-assisted chemical etching of Si to form Si nanowire arrays. These surfaces were chemically modified and rendered hydrophobic by fluorosilane deposition. Au nanoparticles with different size distributions resulted in the synthesis of Si nanowires with very different morphologies (i.e., clumped and straight nanowire surfaces). The difference in nanowire morphology is attributed to capillary force-induced nanocohesion, which is due to the difference in nanowire porosity. The clumped nanowire surface demonstrated the lotus effect, and the straighter nanowires demonstrated the ability to pin water droplets while maintaining large contact angles (i.e., the petal effect). The high contact angles in both cases are explained by invoking the Cassie-Baxter wetting state. The high adhesion behavior of the straight nanowire surface may be explained by a combination of attractive van der Waals forces and capillary adhesion. We demonstrate the spatial patterning of both low- and high-adhesion superhydrophobicity on the same substrate by the simultaneous synthesis of clumped and straight silicon nanowires. The demonstration of hybrid superhydrophobic surfaces with spatially selective, tunable adhesion behavior on single substrates paves the way for future applications in microfluidic channels, substrates for biologically and chemically based analysis and detection where it is necessary to analyze a particular droplet in a defined location on a surface, and as a platform to study in situ chemical mixing and interfacial reactions of liquid pearls.

  6. A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi1

    Science.gov (United States)

    Guether, Mike; Neuhäuser, Benjamin; Balestrini, Raffaella; Dynowski, Marek; Ludewig, Uwe; Bonfante, Paola

    2009-01-01

    In mycorrhizal associations, the fungal partner assists its plant host by providing nitrogen (N) in addition to phosphate. Arbuscular mycorrhizal (AM) fungi have access to inorganic or organic forms of N and translocate them via arginine from the extra- to the intraradical mycelium, where the N is transferred to the plant without any carbon skeleton. However, the molecular form in which N is transferred, as well as the involved mechanisms, is still under debate. NH4+ seems to be the preferential transferred molecule, but no plant ammonium transporter (AMT) has been identified so far. Here, we offer evidence of a plant AMT that is involved in N uptake during mycorrhiza symbiosis. The gene LjAMT2;2, which has been shown to be the highest up-regulated gene in a transcriptomic analysis of Lotus japonicus roots upon colonization with Gigaspora margarita, has been characterized as a high-affinity AMT belonging to the AMT2 subfamily. It is exclusively expressed in the mycorrhizal roots, but not in the nodules, and transcripts have preferentially been located in the arbusculated cells. Yeast (Saccharomyces cerevisiae) mutant complementation has confirmed its functionality and revealed its dependency on acidic pH. The transport experiments using Xenopus laevis oocytes indicated that, unlike other plant AMTs, LjAMT2;2 transports NH3 instead of NH4+. Our results suggest that the transporter binds charged ammonium in the apoplastic interfacial compartment and releases the uncharged NH3 into the plant cytoplasm. The implications of such a finding are discussed in the context of AM functioning and plant phosphorus uptake. PMID:19329566

  7. Ectopic expression of miR156 represses nodulation and causes morphological and developmental changes in Lotus japonicus.

    Science.gov (United States)

    Wang, Ying; Wang, Zhishuo; Amyot, Lisa; Tian, Lining; Xu, Ziqin; Gruber, Margaret Y; Hannoufa, Abdelali

    2015-04-01

    The effects of microRNA156 overexpression on general plant architecture, branching, flowering time and nodulation were investigated in the model legume, Lotus japonicus. We cloned an miR156 homolog, LjmiR156a, from L. japonicus, and investigated its SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes and its biological function at enhancing vegetative biomass yield, extending flowering time, and its impact on nodulation. Thirteen potential targets for LjmiR156 were identified in vitro and their expression profiles were determined in aerial and underground parts of mature plants, including genes coding for eight SPLs, one WD-40, one RNA-directed DNA polymerase, two transport proteins, and one histidine-phosphotransfer protein. Two SPL and one WD-40 cleavage targets for LjmiR156-TC70253, AU089191, and TC57859-were identified. Transgenic plants with ectopic expression of LjmiR156a showed enhanced branching, dramatically delayed flowering, underdeveloped roots, and reduced nodulation. We also examined the transcript levels of key genes involved in nodule organogenesis and infection thread formation to determine the role of miR156 in regulating symbiosis. Overexpression of LjmiR156a led to repression of several nodulation genes during the early stages of root development such as three ENOD genes, SymPK, POLLUX, CYCLOPS, Cerberus, and Nsp1, and the stimulation of NFR1. Our results show that miR156 regulates vegetative biomass yield, flowering time and nodulation by silencing downstream target SPLs and other genes, suggesting that the miR156 regulatory network could be modified in forage legumes (such as alfalfa and trefoils) and in leafy vegetables (like lettuce and spinach) to positively impact economically valuable crop species.

  8. Biosynthesis of the Nitrile Glucosides Rhodiocyanoside A and D and the Cyanogenic Glucosides Lotaustralin and Linamarin in Lotus japonicus1

    Science.gov (United States)

    Forslund, Karin; Morant, Marc; Jørgensen, Bodil; Olsen, Carl Erik; Asamizu, Erika; Sato, Shusei; Tabata, Satoshi; Bak, Søren

    2004-01-01

    Lotus japonicus was shown to contain the two nitrile glucosides rhodiocyanoside A and rhodiocyanoside D as well as the cyanogenic glucosides linamarin and lotaustralin. The content of cyanogenic and nitrile glucosides in L. japonicus depends on plant developmental stage and tissue. The cyanide potential is highest in young seedlings and in apical leaves of mature plants. Roots and seeds are acyanogenic. Biosynthetic studies using radioisotopes demonstrated that lotaustralin, rhodiocyanoside A, and rhodiocyanoside D are derived from the amino acid l-Ile, whereas linamarin is derived from Val. In silico homology searches identified two cytochromes P450 designated CYP79D3 and CYP79D4 in L. japonicus. The two cytochromes P450 are 94% identical at the amino acid level and both catalyze the conversion of Val and Ile to the corresponding aldoximes in biosynthesis of cyanogenic glucosides and nitrile glucosides in L. japonicus. CYP79D3 and CYP79D4 are differentially expressed. CYP79D3 is exclusively expressed in aerial parts and CYP79D4 in roots. Recombinantly expressed CYP79D3 and CYP79D4 in yeast cells showed higher catalytic efficiency with l-Ile as substrate than with l-Val, in agreement with lotaustralin and rhodiocyanoside A and D being the major cyanogenic and nitrile glucosides in L. japonicus. Ectopic expression of CYP79D2 from cassava (Manihot esculenta Crantz.) in L. japonicus resulted in a 5- to 20-fold increase of linamarin content, whereas the relative amounts of lotaustralin and rhodiocyanoside A/D were unaltered. PMID:15122013

  9. Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in Lotus japonicus.

    Science.gov (United States)

    Forslund, Karin; Morant, Marc; Jørgensen, Bodil; Olsen, Carl Erik; Asamizu, Erika; Sato, Shusei; Tabata, Satoshi; Bak, Søren

    2004-05-01

    Lotus japonicus was shown to contain the two nitrile glucosides rhodiocyanoside A and rhodiocyanoside D as well as the cyanogenic glucosides linamarin and lotaustralin. The content of cyanogenic and nitrile glucosides in L. japonicus depends on plant developmental stage and tissue. The cyanide potential is highest in young seedlings and in apical leaves of mature plants. Roots and seeds are acyanogenic. Biosynthetic studies using radioisotopes demonstrated that lotaustralin, rhodiocyanoside A, and rhodiocyanoside D are derived from the amino acid l-Ile, whereas linamarin is derived from Val. In silico homology searches identified two cytochromes P450 designated CYP79D3 and CYP79D4 in L. japonicus. The two cytochromes P450 are 94% identical at the amino acid level and both catalyze the conversion of Val and Ile to the corresponding aldoximes in biosynthesis of cyanogenic glucosides and nitrile glucosides in L. japonicus. CYP79D3 and CYP79D4 are differentially expressed. CYP79D3 is exclusively expressed in aerial parts and CYP79D4 in roots. Recombinantly expressed CYP79D3 and CYP79D4 in yeast cells showed higher catalytic efficiency with l-Ile as substrate than with l-Val, in agreement with lotaustralin and rhodiocyanoside A and D being the major cyanogenic and nitrile glucosides in L. japonicus. Ectopic expression of CYP79D2 from cassava (Manihot esculenta Crantz.) in L. japonicus resulted in a 5- to 20-fold increase of linamarin content, whereas the relative amounts of lotaustralin and rhodiocyanoside A/D were unaltered.

  10. Contestation of ‘the holy places in the Zimbabwean Religious Landscape’: A study of the Johane Masowe Chishanu yeNyenyedzi Church’s sacred places

    Directory of Open Access Journals (Sweden)

    Phillip Musoni

    2016-08-01

    Full Text Available Places that are regarded as holy are highly esteemed in most religious institutions. Such places are revered because they denote the converging points of human beings and the divine. The fundamental questions addressed in this study are: what makes a place holy? Do Christians share sacred places with other religious groups? The study theorises that the Johane Masowe Chishanu yeNyenyedzi Church has forcefully appropriated most of the African indigenous scared places such as hills, shades and dams for all-night prayers and water baptisms. The researcher has selected two indigenous religious shrines; Chivavarira hill and Gonawapotera pool of Chirumhanzu located in the Midlands Province of Zimbabwe. The two shrines are regarded by the indigenes as renowned and sacred. This study analyses and thereto seeks to decode deeper on what makes the Johane Masowe Chishanu yeNyenyedzi Church to enthusiastically appropriate most of the African indigenous shrines and, to some extent, turn them to be their shrines. It is this insight which makes the two shrines to be contested places, especially as perceived from both the indigenes and Christian perspectives. Therefore, this study is a contemporary issue that constitutes the focus of the present concerns. Accordingly, in order to archive the intended goal, this research study relies heavily on participant observation and interviews for data collection, since there is hardly documentation readily available about the Masowe yeNyenyedzi Church in Zimbabwe.

  11. Le neurofibrome pré sacré solitaire géant: cause rare de masse pelvienne chez la femme

    Science.gov (United States)

    Boujoual, Majdouline; Hachi, Hafid; Merrouni, Mohammed Alami; El khannoussi, Basma; Bougtab, Abdeslam

    2014-01-01

    Le neurofibrome présacré solitaire est une tumeur ectodermiquetrès rare tant par sa fréquence que par sa localisation, souvent pauci-symptomatiquejusqu’à l'atteinte de taille importante, d'accès difficile et de diagnostic erroné. L'imagerie préopératoirejoue un rôle essentieldans la prise en charge. Son diagnostic est immuno-histologique. Son traitement chirurgical est basé sur l'exérèse complète à marges saines. Nous rapportons l'observation d'une patiente de 46 ans, ayant été opérée pour suspicion de fibrome utérin sous séreux, dont l'exploration chirurgicale a confirméune tumeur rétropéritonéale et présacrée. L'IRM post opératoire a précisé ses rapports anatomiques. La reprise chirurgicalea permis l'exérèse complète de la tumeur sans lésions des organes adjacents. L'histologie et l’étude immuno-histochimique ont conclu à un neurofibrome. PMID:25328584

  12. Effects of exotic Eucalyptus spp. plantations on soil properties in and around sacred natural sites in the northern Ethiopian Highlands

    Directory of Open Access Journals (Sweden)

    Alemayehu Wassie

    2016-04-01

    Full Text Available Species of the genus Eucalyptus (common name eucalyptus are widely planted all across Ethiopia—including on large areas of land previously allocated to food production. In recent decades eucalyptus has also increasingly been planted on lands around and within “church forests,” sacred groves of old-aged Afromontane trees surrounding Ethiopian Orthodox Tewahido churches. These revered holy sites have long been recognized for their cultural values and also for their ecosystem services—including their potential to support species conservation and restoration, as church forests are some of the only remaining sanctuaries for many of Ethiopia’s indigenous and endemic plant and animal populations. Ethiopian Orthodox church communities have a long history of planting and nurturing indigenous tree seedlings to sustain church forest groves. However, due to the fast-growing nature of eucalyptuscombined with its widely recognized socio-economic benefits (as fuelwood, charcoal, construction wood, etc., this introduced species has been widely plantedaround church forests—in some cases even replacing native tree species within church forests themselves. In many developing country contexts the introduction of exotic eucalyptus has been shown to have ecological impacts ranging from soil nutrient depletion, to lowering water tables, to allelopathic effects. In this study, we collected soil samples from indigenous forest fragments (church forests, adjacent eucalyptus plantations, and surrounding agricultural land to examine how eucalyptus plantations in Ethiopian Orthodox church communitiesmight impact soil quality relative to alternative land uses. Soil properties, including organic matter, pH, total nitrogen, and total phosphorus were measured in samples across 20 church forest sites in South Gondar, East Gojjam, West Gojjam, Awi, and Bahir Dar Liyu zones in the Amhara Region of the northern Ethiopian Highlands. Findings indicate that although soil in

  13. Le sacre du printemps

    Directory of Open Access Journals (Sweden)

    Denise Pumain

    2002-03-01

    Full Text Available Cybergeo aura six ans en avril : dans la réalité du virtuel, dans l'univers récent et fluctuant de la publication en ligne, cela fait de nous, tout à la fois, des pionniers et des vétérans. De façon plus surprenante, il se trouve que nous sommes aussi uniques : parmi toutes les revues électroniques de sciences sociales, aucune ne combine comme Cybergeo ancienneté, publication exclusivement électronique, liberté d'accès au texte intégral, édition et gestion par des chercheurs, et comité de lec...

  14. Light in sacred architecture

    Directory of Open Access Journals (Sweden)

    Jurij Kryworuczko

    2014-11-01

    Full Text Available Described are the traditional means for the embodiment of theological and architectural nature of light in the spatial organization of Christian churches. Basic principles and tools for the spatial organization of lighting environment in the Ukrainian temple buildings are given. The importance of natural and artificial light for the creation of structure and space of the church is found. Revealed are the regularities for the church lighting environment in the temporal dynamics of worshiping; disclosed are the tools to transfer principles of the traditional church lighting practices to modern temples.

  15. The Sacred Frankincense

    African Journals Online (AJOL)

    (HPTLC) is becoming a technique for the routine analysis for the identification of medicinal plants and derived ... Water used for buffer preparation was deionized using Simplicity water purification system (Millipore). ... Thereafter,. 0.5 and 2 μL of each sample were applied as bands of 8 mm using Automatic TLC sampler 4.

  16. Efficacy and safety of the Lotus Valve System for treatment of patients with severe aortic valve stenosis and intermediate surgical risk

    DEFF Research Database (Denmark)

    De Backer, Ole; Götberg, Matthias; Ihlberg, Leo

    2016-01-01

    BACKGROUND: Transcatheter aortic valve replacement (TAVR) has become an established therapeutic option for patients with symptomatic, severe aortic valve stenosis (AS) who are ineligible or at high risk for conventional valvular surgery. In Northwestern Europe, the TAVR technology is also......)-defined device success was obtained in 97.4%. A Lotus Valve was successfully implanted in all patients. There was no valve migration, embolization, ectopic valve deployment, or TAV-in-TAV deployment. The VARC-defined combined safety rate at 30days was 92.2%, with a mortality rate of 1.9% and stroke rate of 3.......2%. The clinical efficacy rate after 30days was 91.6% - only one patient had moderate aortic regurgitation. When considering only those patients in the late experience group (N=79), the combined safety and clinical efficacy rates were 93.7% and 92.4%, respectively. The pacemaker implantation rate was 27...

  17. Mesorhizobium bacterial strains isolated from the legume Lotus corniculatus are an alternative source for the production of polyhydroxyalkanoates (PHAs) to obtain bioplastics.

    Science.gov (United States)

    Marcos-García, Marta; García-Fraile, Paula; Filipová, Alena; Menéndez, Esther; Mateos, Pedro F; Velázquez, Encarna; Cajthaml, Tomáš; Rivas, Raúl

    2017-07-01

    Polyhydroxyalkanoic acids (PHAs) are natural polyesters that can be used to produce bioplastics which are biodegradable. Numerous microorganisms accumulate PHAs as energy reserves. Combinations of different PHAs monomers lead to the production of bioplastics with very different properties. In the present work, we show the capability of strains belonging to various phylogenetic lineages within the genus Mesorhizobium, isolated from Lotus corniculatus nodules, to produce different PHA monomers. Among our strains, we found the production of 3-hydroxybutyrate, 3-hydroxyvalerate, 3-hydroxydodecanoate, and 3-hydroxyhexadecanoate. Most of the PHA-positive strains were phylogenetically related to the species M. jarvisii. However, our findings suggest that the ability to produce different monomers forming PHAs is strain-dependent.

  18. A low-cost filler-dissolved process for fabricating super-hydrophobic poly(dimethylsiloxane) surfaces with either lotus or petal effect

    Science.gov (United States)

    Lin, Yung-Tsan; Chou, Jung-Hua

    2014-05-01

    A low-cost filler (salt) water-dissolved method is developed to produce large-area and flexible super-hydrophobic surfaces by using poly(dimethylsiloxane) (PDMS) material. Five levels of salt grain sizes are used to examine the filler size effect on fabricating the super-hydrophobic surfaces and on the hydrophobic mechanism involved. The results show that the surfaces fabricated using grain sizes of 53-74 and 74-104 µm exhibit the lotus effect (cell adhesion (CA) > 150° and self-adhesion (SA) 150° and high adhesion even upside-down). The super-hydrophobic characteristic is achieved mainly by the large micro rib-like structures, small micro rock-like bumps, and textures on the bump due to the fillers.

  19. Glycolysis and the Tricarboxylic Acid Cycle Are Linked by Alanine Aminotransferase during Hypoxia Induced by Waterlogging of Lotus japonicus1[W][OA

    Science.gov (United States)

    Rocha, Marcio; Licausi, Francesco; Araújo, Wagner L.; Nunes-Nesi, Adriano; Sodek, Ladaslav; Fernie, Alisdair R.; van Dongen, Joost T.

    2010-01-01

    The role of nitrogen metabolism in the survival of prolonged periods of waterlogging was investigated in highly flood-tolerant, nodulated Lotus japonicus plants. Alanine production revealed to be a critical hypoxic pathway. Alanine is the only amino acid whose biosynthesis is not inhibited by nitrogen deficiency resulting from RNA interference silencing of nodular leghemoglobin. The metabolic changes that were induced following waterlogging can be best explained by the activation of alanine metabolism in combination with the modular operation of a split tricarboxylic acid pathway. The sum result of this metabolic scenario is the accumulation of alanine and succinate and the production of extra ATP under hypoxia. The importance of alanine metabolism is discussed with respect to its ability to regulate the level of pyruvate, and this and all other changes are discussed in the context of current models concerning the regulation of plant metabolism. PMID:20089769

  20. PII Overexpression in Lotus japonicus Affects Nodule Activity in Permissive Low-Nitrogen Conditions and Increases Nodule Numbers in High Nitrogen Treated Plants.

    Science.gov (United States)

    D'Apuzzo, Enrica; Valkov, Vladimir Totev; Parlati, Aurora; Omrane, Selim; Barbulova, Ani; Sainz, Maria Martha; Lentini, Marco; Esposito, Sergio; Rogato, Alessandra; Chiurazzi, Maurizio

    2015-04-01

    We report here the first characterization of a GLNB1 gene coding for the PII protein in leguminous plants. The main purpose of this work was the investigation of the possible roles played by this multifunctional protein in nodulation pathways. The Lotus japonicus LjGLB1 gene shows a significant transcriptional regulation during the light-dark cycle and different nitrogen availability, conditions that strongly affect nodule formation, development, and functioning. We also report analysis of the spatial profile of expression of LjGLB1 in root and nodule tissues and of the protein's subcellular localization. Transgenic L. japonicus lines overexpressing the PII protein were obtained and tested for the analysis of the symbiotic responses in different conditions. The uncoupling of PII from its native regulation affects nitrogenase activity and nodule polyamine content. Furthermore, our results suggest the involvement of PII in the signaling of the nitrogen nutritional status affecting the legumes' predisposition for nodule formation.

  1. Quality Degradation of Chinese White Lotus Seeds Caused by Dampening during Processing and Storage: Rapid and Nondestructive Discrimination Using Near-Infrared Spectroscopy

    Directory of Open Access Journals (Sweden)

    Lu Xu

    2015-01-01

    Full Text Available Dampening during processing or storage can largely influence the quality of white lotus seeds (WLS. This paper investigated the feasibility of using near-infrared (NIR spectroscopy and chemometrics for rapid and nondestructive discrimination of the dampened WLS. Regular (n=167 and dampened (n=118 WLS objects were collected from five main producing areas and NIR reflectance spectra (4000–12000 cm−1 were measured for bare kernels. The influence of spectral preprocessing methods, including smoothing, taking second-order derivatives (D2, and standard normal variate (SNV, on partial least squares discrimination analysis (PLSDA was compared to select the optimal data preprocessing method. A moving-window strategy was combined with PLSDA (MWPLSDA to select the most informative wavelength intervals for classification. Based on the selected spectral ranges, the sensitivity, specificity, and accuracy were 0.927, 0.950, and 0.937 for SNV-MWPLSDA, respectively.

  2. Industry Application ECCS / LOCA Integrated Cladding/Emergency Core Cooling System Performance: Demonstration of LOTUS-Baseline Coupled Analysis of the South Texas Plant Model

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Hongbin [Idaho National Lab. (INL), Idaho Falls, ID (United States); Szilard, Ronaldo [Idaho National Lab. (INL), Idaho Falls, ID (United States); Epiney, Aaron [Idaho National Lab. (INL), Idaho Falls, ID (United States); Parisi, Carlo [Idaho National Lab. (INL), Idaho Falls, ID (United States); Vaghetto, Rodolfo [Texas A & M Univ., College Station, TX (United States); Vanni, Alessandro [Texas A & M Univ., College Station, TX (United States); Neptune, Kaleb [Texas A & M Univ., College Station, TX (United States)

    2017-06-01

    Under the auspices of the DOE LWRS Program RISMC Industry Application ECCS/LOCA, INL has engaged staff from both South Texas Project (STP) and the Texas A&M University (TAMU) to produce a generic pressurized water reactor (PWR) model including reactor core, clad/fuel design and systems thermal hydraulics based on the South Texas Project (STP) nuclear power plant, a 4-Loop Westinghouse PWR. A RISMC toolkit, named LOCA Toolkit for the U.S. (LOTUS), has been developed for use in this generic PWR plant model to assess safety margins for the proposed NRC 10 CFR 50.46c rule, Emergency Core Cooling System (ECCS) performance during LOCA. This demonstration includes coupled analysis of core design, fuel design, thermalhydraulics and systems analysis, using advanced risk analysis tools and methods to investigate a wide range of results. Within this context, a multi-physics best estimate plus uncertainty (MPBEPU) methodology framework is proposed.

  3. Effect of exchanging Onobrychis viciifolia and Lotus corniculatus for Medicago sativa on ruminal fermentation and nitrogen turnover in dairy cows.

    Science.gov (United States)

    Grosse Brinkhaus, A; Bee, G; Silacci, P; Kreuzer, M; Dohme-Meier, F

    2016-06-01

    The objective of the study was to determine the effect of feeding sainfoin (SF; Onobrychis viciifolia) and birdsfoot trefoil (BT; Lotus corniculatus), 2 temperate climate forage legumes that contain condensed tannins (CT), on ruminal fermentation and N turnover in dairy cows. Six ruminally cannulated multiparous dairy cows (milk yield=40kg/d; 36 d in milk) were used in a replicated 3×3 Latin square design. All animals were fed basal diets containing 20% pelleted SF (223g of CT/kg of dry matter), BT (30.3g of CT/kg of dry matter), or alfalfa (AL) and concentrate to meet their predicted nutrient requirements. Each experimental period consisted of a 21-d adaptation period in a tiestall, followed by a 7-d collection period in metabolic crates, where feces and urine were collected quantitatively. During the 7-d period, milk yield was recorded daily and milk samples were taken at each milking. Blood, ruminal fluid, and papillae were sampled on d 2 and 5. The relative abundance of selected bacterial strains in ruminal fluid and the gene expression of transporter genes in the papillae were determined with quantitative PCR. Total volatile fatty acids and the abundance of the cellulolytic bacteria Prevotella spp. and Ruminococcus flavefaciens decreased with SF compared with AL. The relative gene expression of the monocarboxylate transporter 1 was increased with BT compared with AL and SF. Total yields of milk, milk fat, and milk protein were similar among treatments. The proportion of 18:3n-3 in milk fat was greater and those of 22:5n-3 and 22:6n-3 were lower with SF than with BT. The contents of urea N in blood (2.71, 3.45, and 3.90mmol/L for SF, AL, and BT, respectively), milk (79.8, 100.1, and 110.9mg/kg for SF, AL, and BT, respectively), and urine were lower with SF than with AL and BT, and a trend toward a lower ruminal ammonia content occurred with SF compared with BT. Intake and excretion of N with milk were similar among treatments, but urine N was lower with SF

  4. Orchestrated regulation of Nogo receptors, LOTUS, AMPA receptors and BDNF in an ECT model suggests opening and closure of a window of synaptic plasticity.

    Directory of Open Access Journals (Sweden)

    Max Nordgren

    Full Text Available Electroconvulsive therapy (ECT is an efficient and relatively fast acting treatment for depression. However, one severe side effect of the treatment is retrograde amnesia, which in certain cases can be long-term. The mechanisms behind the antidepressant effect and the amnesia are not well understood. We hypothesized that ECT causes transient downregulation of key molecules needed to stabilize synaptic structure and to prevent Ca2+ influx, and a simultaneous increase in neurotrophic factors, thus providing a short time window of increased structural synaptic plasticity. Here we followed regulation of NgR1, NgR3, LOTUS, BDNF, and AMPA subunits GluR1 and GluR2 flip and flop mRNA levels in hippocampus at 2, 4, 12, 24, and 72 hours after a single episode of induced electroconvulsive seizures (ECS in rats. NgR1 and LOTUS mRNA levels were transiently downregulated in the dentate gyrus 2, 4, 12 and 4, 12, 24 h after ECS treatment, respectively. GluR2 flip, flop and GluR1 flop were downregulated at 4 h. GluR2 flip remained downregulated at 12 h. In contrast, BDNF, NgR3 and GluR1 flip mRNA levels were upregulated. Thus, ECS treatment induces a transient regulation of factors important for neuronal plasticity. Our data provide correlations between ECS treatment and molecular events compatible with the hypothesis that both effects and side effects of ECT may be caused by structural synaptic rearrangements.

  5. The Lotus japonicus genome

    DEFF Research Database (Denmark)

    , carbon/nitrogen and secondary metabolism, as well as advances made in high-throughput genomic and genetic approaches. Research focusing on model plants has underpinned the recent growth in plant genomics and genetics and provided a basis for investigations of major crop species. In the legume family...

  6. Lotus Effect Toy

    Science.gov (United States)

    Shakerin, Said

    2011-01-01

    This is a short article about Aqua Drop, a toy that employs a superhydrophobic surface. After defining superhydrophobic surface, its occurrence in nature, and its importance in science and technology, I describe the toy, followed by several student activities that can be performed with the toy and easily found natural objects.

  7. Sacred History for a Central Asian TownSaints, Shrines, and Legends of Origin inHistories of Sayrām, 18th-19th Centuries

    Directory of Open Access Journals (Sweden)

    Devin DeWeese

    2009-05-01

    Full Text Available Cet article étudie les mythes historiques concernant Sayrām, une petite ville du sud de l'actuel Kazakhstan, cas exemplaire de concepts indigènes d'identité communautaire en Asie centrale pré-soviétique. Ces traditions sont conservées dans un ensemble de travaux en turc, sous le titre générique de « Histoire de Sayrām », qui combine une « histoire sacrée » de la ville avec une « géographie sacrée » sous la forme d'un catalogue de mausolées locaux ; ces deux composantes situent ainsi Sayrām à la fois dans le temps et dans l'espace, à l'intérieur d'un univers sacré défini en tant qu'islamique. Elles offrent une vision de la participation de cette ville à un cadre historique déterminé par l'hommage sanctificateur du Prophète Muhammad à Sayrām, et une affirmation de la présence continue et de la protection, à travers leurs mausolées, d'une multitude de saints musulmans (comprenant aussi bien des personnages purement locaux que d'autres bien connus dans le folklore islamique. Ces ouvrages, compilés vraisemblablement au XVIIIe siècle et répandus très largement dans la seconde moitié du XIXe siècle, reflètent souvent d'anciennes traditions narratives évocatrices du rôle de Sayrām au début de l'islamisation de l'Asie centrale. Mais elles montrent également l'importance capitale de la définition par la religion des identités locales et régionales, au sein de la population sédentaire d'Asie centrale, avant les changements induits par la conquête russe, l'établissement du pouvoir soviétique et la formulation des nouvelles identités « nationales » qui perdurent aujourd'hui.

  8. Traditional medicine applied by the Saraguro yachakkuna: a preliminary approach to the use of sacred and psychoactive plant species in the southern region of Ecuador.

    Science.gov (United States)

    Armijos, Chabaco; Cota, Iuliana; González, Silvia

    2014-02-24

    During the colonial period, the indigenous saraguros maintained their traditions, knowledge, and practices to restore and preserve the health of their members. Unfortunately, many of their practices and medicinal resources have not been documented. In this study, we sought to document the traditional healers' (yachakkuna saraguros) knowledge about medicinal and psychoactive plants used in the mesas and in magical-religious rituals. The study was conducted under a technical and scientific cooperation agreement between the Universidad Técnica Particular de Loja (UTPL), the Dirección Provincial de Salud de Loja (DPSL), and the Saraguro Healers Council (Consejo de Sanadores de Saraguro). For the present study, the DPSL and Saraguro Healers Council selected the 10 yachakkuna most recognized for their knowledge and their use of sacred and psychoactive species. Ten interviews with the selected yachakkuna were conducted between 2010 and 2011 to ascertain how the Saraguro traditional healing system is structured and to obtain a record of the sacred and medicinal plant species used to treat supernatural diseases and for psychoactive purposes. The present study describes the traditional health system in the Saraguro indigenous community located in southern Ecuador. It also describes the main empirical methods used to diagnose diseases: direct physical examination of the patient, observation of the patient's urine, documentation of the patient's pulse, limpia, palpation and visionary methods, including supernatural diseases (susto, vaho de agua, mal aire, mal hecho, shuka) and reports of the use of sacred and medicinal psychoactive plants, such as the San Pedro cactus (Echinopsis pachanoi), wandug (Brugmansia spp.), and tobacco (Nicotiana spp.). This study also describes the rituals (limpia, soplada) employed by the Saraguro yachakkuna to treat supernatural diseases. Finally, we report on the main plants used during limpia in the Saraguro community. The current traditional

  9. Christian sacred architecture of late antique Romuliana (IV-VII c.: Desacralization of an imperial memorial palace

    Directory of Open Access Journals (Sweden)

    Špehar Olga

    2011-01-01

    the new church was raised on the same spot. In the second half of the fifth century, room R of the Palace I was transformed into the small single-nave church by building the new apse on its eastern end. It was connected with the small room M, which was transformed into the baptistery by building the small cross-shaped piscina. By the beginning of the sixth century the small room M was added an apse in the east, so it was also transformed into the church. Basilica I was destroyed and the new church was built in the same place most probably by the middle of the sixth century, presumably at the time of the restoration of the fortresses along the limes and in the hinterland during the reign of Justinian I (527-565. Procopius informs us that Romuliana was among the renovated fortresses and that it fell under the jurisdiction of the Episcopal see in Aquis. The newly built three-aisled Basilica II was wider than the older one and had a quatrefoil baptistery at its south-western side. Geographically and chronologically, closest parallels for this architectural type of baptistery would be the quatrefoils baptisteries in Stobi and Caričin grad (Iustiniana Prima. The existence of the baptistery, made some researchers connect Romuliana with the episcopate of Meridio or define settlement as an Episcopal see of the sixth century, but the available archeological data do not permit any precise hypothesis referring its place in church organization. Despite the lack of the information in the written sources, the number of Christian sacred temples on the site of Late Antique Romuliana testify of the size of its Christian community in the first three centuries of the official Christianity.

  10. Stroke Avoidance for Children in REpública Dominicana (SACRED): Protocol for a Prospective Study of Stroke Risk and Hydroxyurea Treatment in Sickle Cell Anemia.

    Science.gov (United States)

    Jeste, Neelum D; Sánchez, Luisanna M; Urcuyo, Gabriela S; Bergés, Melissa E; Luden, Judy P; Stuber, Susan E; Latham, Teresa S; Mena, Rafael; Nieves, Rosa M; Ware, Russell E

    2017-06-02

    In the Dominican Republic, where the burden of sickle cell anemia (SCA) is high, many children lack access to routine screening and preventative care. Children with SCA are at risk for stroke, an event that leads to significant morbidity and mortality. In the United States, screening via transcranial Doppler (TCD) identifies children with SCA at highest stroke risk, allowing early intervention with blood transfusions. The need for indefinite transfusions for primary stroke prevention limits their practicality in limited-resource countries. Hydroxyurea has been shown to lower TCD velocities and to prevent conversion from conditional (170 to 199 cm/sec) to abnormal (greater than or equal to 200 cm/sec) velocities. In resource-limited settings, implementation of a TCD screening program, coupled with hydroxyurea therapy, could reduce the burden of SCA and stroke. The aims of the Stroke Avoidance for Children in REpública Dominicana (SACRED) trial are (1) to screen children with SCA for stroke risk using TCD and to determine the prevalence of elevated velocities in a cross-sectional sample; (2) to identify clinical and laboratory correlates of elevated velocities; and (3) to obtain longitudinal data on the natural history of TCD velocities and to measure therapeutic effects of hydroxyurea. This prospective trial, designed and conducted by Cincinnati Children's Hospital Medical Center (CCHMC) and Hospital Infantil Robert Reid Cabral (HIRRC) with Centro de Obstetricia y Ginecología, includes a baseline cross-sectional epidemiological survey of the distribution of TCD velocities across a large cohort of children with SCA in the Dominican Republic. Children with conditional velocities are eligible to begin protocol-directed hydroxyurea if laboratory criteria are met. The treatment schedule begins with a fixed-dose of approximately 20 mg/kg/day for 6 months, after which it escalates to maximum tolerated dose (MTD). All participants undergo longitudinal annual TCD

  11. Temporal, but not spatial, changes in expression patterns of petal identity genes are associated with loss of papillate conical cells and the shift to bird pollination in Macaronesian Lotus (Leguminosae).

    Science.gov (United States)

    Ojeda, D I; Jaén-Molina, R; Santos-Guerra, A; Caujape-Castells, J; Cronk, Q

    2017-05-01

    In the generally bee-pollinated genus Lotus a group of four species have evolved bird-pollinated flowers. The floral changes in these species include altered petal orientation, shape and texture. In Lotus these characters are associated with dorsiventral petal identity, suggesting that shifts in the expression of dorsal identity genes may be involved in the evolution of bird pollination. Of particular interest is Lotus japonicus CYCLOIDEA 2 (LjCYC2), known to determine the presence of papillate conical cells on the dorsal petal in L. japonicus. Bird-pollinated species are unusual in not having papillate conical cells on the dorsal petal. Using RT-PCR at various stages of flower development, we determined the timing of expression in all petal types for the three putative petal identity genes (CYC-like genes) in different species with contrasting floral morphology and pollination syndromes. In bird-pollinated species the dorsal identity gene, LjCYC2, is not expressed at the floral stage when papillate conical cells are normally differentiating in bee-pollinated species. In contrast, in bee-pollinated species, LjCYC2 is expressed during conical cell development. Changes in the timing of expression of the above two genes are associated with modifications in petal growth and lateralisation of the dorsal and ventral petals in the bird-pollinated species. This study indicates that changes in the timing, rather than spatial distribution, of expression likely contribute to the modifications of petal micromorphology and petal size during the transition from bee to bird pollination in Macaronesian Lotus species. © 2017 German Botanical Society and The Royal Botanical Society of the Netherlands.

  12. Dans les flots de l'Ao-Shio. Entre Japon et Corée : de l'îlot sacré à l'îlot disputé

    OpenAIRE

    Pelletier, P.

    1998-01-01

    Two small islands located in the Ao-shio (or Tsushima) sea current are facing two opposite fates reflecting the highs-lows of the relationship between Korea and Japan, either peaceful or warlike. Okinoshima, which is incorporated within the Japanese world, is a sacred island. Takeshima or Tok-to is being fought over by Japanese and Korean states. (Résumé d'auteur)

  13. Liturgy and Morality. Constitution  on the Sacred Liturgy Sacrosanctum Concilium in the Context of Moral Theology

    Directory of Open Access Journals (Sweden)

    Bogusław Mielec

    2013-09-01

    Full Text Available The fiftieth anniversary of the promulgation of the Constitution on the Sacred Liturgy of Vaticanum II encourages attempts of its interpretation in the context of moral theology. It leads to the conclusion that there is a co-relation between liturgy and morality in the history of salvation. The Revelation of the Old Testament reveals the conjunction between worship and morality. Attempts to separate the two in the history of the Chosen People raise objections of prophets. Their criticism, however, comes together with the announcement of the future in which God will reveal the divisions between worship and morality. The New Testament shows the fulfilment of this promise in Jesus Christ. His salvific work establishes the Christian liturgy, in which man participates as a physical, as well as a spiritual being. The salvation, made present in the liturgy of the Church, is a relation, the co-operation of God and man, grace and freedom. Sacrosantum Concilium presents this revealed truth, emphasising the objective and subjective dimensions of liturgy as the work of Christ, in which a Christian participates actively, freely and consciously (participatio actuosa. The participation is mostly spiritual, therefore moral at the same time. Only than can the external elements of liturgy take place, including its detailed forms, gestures, attitudes and behaviours of the sacred liturgy participants. It means that the liturgical participatio actuosa requires moral works through imitation of Christ leading to conversion. That is when “faith works through love” (cf. Gal 5 : 6. It leads us to the notion of sacrifice, which, as far as man is concerned, requires a total change. Sinful man presented with the love of the Triune God may respond to it with his own, gradually maturing love. The process constitutes the drama of God and man, which is the tension between grace and freedom, which is then represented by the co-relation between liturgy and morality

  14. Authentication of lotus root powder adulterated with potato starch and/or sweet potato starch using Fourier transform mid-infrared spectroscopy.

    Science.gov (United States)

    Liu, Jia; Wen, Yu; Dong, Nan; Lai, Chunli; Zhao, Guohua

    2013-12-01

    Fourier transform mid infrared (FT-MIR) spectroscopy combined with chemometrics techniques were developed for classification and quantification of cheaper starches (potato and sweet potato starch) in lotus root powder (LRP). By performing principal component analysis (PCA), it was possible to distinguish between adulterated and non-adulterated LRP. The coefficient of determination (R(2)) and standard deviation ratio (SDR) of calibration set were found to be 0.9587-0.9898 and 3.63-10.2, depending on the pre-treatment of spectra. The external validation set gave a coefficient of determination (R(2)) and standard deviation ratio (SDR) of 0.9810 and 5.47, respectively. Moreover, the limit of detection (1%), the limit of quantification (3%), reasonable recovery (92.3-101.5%), satisfactory intra-assay (2.9-5.5%) and inter-assay (11.0-13.5%) precision illustrated the good performance of the present method. The results obtained in this study indicate that FT-MIR spectroscopy can be used as an easy, rapid and novel tool to detect the LRP adulterated with cheaper starches. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector

    Directory of Open Access Journals (Sweden)

    Nikolić Radomirka

    2007-01-01

    Full Text Available Cotyledons from 6-day-old Lotus corniculatus cv. Bokor seedlings, transversally cut into two halves, were capa­ble of regenerating buds without intervening callus formation. The explants were co-cultivated with the Agrobacterium tumefaciens LBA4404/pTOK233 superbinary vector carrying the uidA-intron gene and the genes hpt and nptII. They were cultured for 14 days on a regeneration medium, then subjected to a stepwise hygromycin B selection procedure consisting of gradually increasing antibiotic concentrations (5-15 mg L-1 over 21 weeks. Transformed shoots were obtained within 5 months after co-cultivation. Out of 124 initially co-cultivated explants, 52 (42% plants survived hygromycin B selection. The presence of transgenes in regenerated plants was verified by β-glucuronidase histochemical assays and PCR analysis for the presence of uidA gene sequences. Hygromycin B-resistant and PCR-positive T0 plants were cultured in the greenhouse to produce flowers and seeds. The obtained data demonstrate that the reported transformation protocol could be useful for introducing agriculturally important genes into the new L. corniculatus cultivar Bokor.

  16. From ‘petal effect’ to ‘lotus effect’ on the highly flexible Silastic S elastomer microstructured using a fluorine based reactive ion etching process

    Science.gov (United States)

    Frankiewicz, Christophe; Zoueshtiagh, Farzam; Talbi, Abdelkrim; Streque, Jérémy; Pernod, Philippe; Merlen, Alain

    2014-11-01

    A fluorine-based reactive ion etching (RIE) process has been applied on a new family of silicone elastomers named ‘Silastic S’ for the first time. Excellent mechanical properties are the principal advantage of this elastomer. The main objective of this study was (i) to develop a new process with an electrodeposited thin Nickel (Ni) layer as a mask to obtain a more precise pattern transfer for deep etching (ii) to investigate the etch rates and the etch profiles obtained under various plasma conditions (gas mixture ratios and pressure). The resulting process exhibits etch rates that range from 20 µm h-1 to 40 µm h-1. The process was optimized to obtain anisotropic profiles of the edges. Finally, it is shown that (iii) the wetting contact angle could be easily modified with this process from 103° to 162°, with a hysteresis that ranges from 2° to 140°. The process is, at present, the only reported solution to reproduce the ‘petal effect’ (high contact angle hysteresis value) on a highly flexible substrate. A possibility to control the contact angle hysteresis from the ‘petal effect’ to the ‘lotus effect’ (low contact angle hysteresis value) has been investigated to allow a precise control on the required energy to pin or unpin the contact line of water droplets. This opens multiple possibilities to exploit this elastomer in many microfluidics applications.

  17. Diospyros lotus leaf and grapefruit stem extract synergistically ameliorate atopic dermatitis-like skin lesion in mice by suppressing infiltration of mast cells in skin lesions.

    Science.gov (United States)

    Cho, Byoung Ok; Che, Denis Nchang; Yin, Hong Hua; Shin, Jae Young; Jang, Seon Il

    2017-05-01

    Atopic dermatitis, a chronic relapsing and pruritic inflammation of the skin also thought to be involved in, or caused by immune system destruction is an upsetting health problem due to its continuously increasing incidence especially in developed countries. Mast cell infiltration in atopic dermatitis skin lesions and its IgE-mediated activation releases various cytokines and chemokines that have been implicated in the pathogenesis of atopic dermatitis. This study was aimed at investigating synergistic anti-inflammatory, anti-pruritic and anti-atopic dermatitis effects of Diospyros lotus leaf extract (DLE) and Muscat bailey A grapefruit stem extract (GFSE) in atopic dermatitis-like induced skin lesions in mice. Combinations of DLE and GFSE inhibited TNF-α and IL-6 production more than DLE or GFSE in PMA plus calcium ionophore A23187-activated HMC-1 cells. DLE and GFSE synergistically inhibited compound 48/80-induced dermal infiltration of mast cells and reduced scratching behavior than DLE or GFSE. Furthermore, DLE and GFSE synergistically showed a stronger ameliorative effect in skin lesions by reducing clinical scores; dermal infiltration of mast cells; ear and dorsal skin thickness; serum IgE and IL-4 production in atopic dermatitis-like mice. Collectively, these results suggest that DLE and GFSE synergistically exhibit anti-atopic dermatitis effects in atopic dermatitis-like skin lesions in mice. Copyright © 2017. Published by Elsevier Masson SAS.

  18. A superparamagnetic Fe3O4-graphene oxide nanocomposite for enrichment of nuciferine in the extract of Nelumbinis Folium (Lotus leaf)

    International Nuclear Information System (INIS)

    Fan, Jie-Ping; Zheng, Bing; Qin, Yu; Yang, Dan; Liao, Dan-Dan; Xu, Xiao-Kang; Zhang, Xue-Hong; Zhu, Jian-Hang

    2016-01-01

    Graphical abstract: - Highlights: • A superparamagnetic Fe3O4-graphene oxide (MGO) nanocomposite was prepared. • It is characterized by TEM, XPS, VSM, XRD and Raman spectroscopy. • The adsorption kinetics, isotherms and reusability of MGO were also investigated. • MGO was applied to enrich nuciferine in the extract of Nelumbinis Folium. - Abstract: In this work, a superparamagnetic Fe 3 O 4 -graphene oxide (MGO) nanocomposite was prepared by one-step chemical co-precipitation method, and characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), powder X-ray diffraction (PXRD), Raman spectroscopy and nitrogen adsorption–desorption curve. The as-prepared MGO was used to adsorb nuciferine, and the adsorption kinetic, isotherm and reusability of MGO were also investigated. The results showed that the adsorption of nuciferine on MGO reached its equilibrium very quickly (within 10 min) due to the two-dimensional carbon nanostructure of GO. In comparison with MGO, five conventional sorbents, i.e., macroporous resin D-101, silica gel, reverse phase silica gel (RP-C18) and cation exchange resin and polyamide, were also used to evaluate their adsorption capabilities. Therefore, MGO combined the advantages of both superparamagnetic particle and GO, i.e., easy separation and high absorption capacity. Finally, MGO was successfully applied to enrichment and separation of nuciferine in the extract of Nelumbinis Folium (Lotus leaf).

  19. Symbiotic competence in Lotus japonicus is affected by plant nitrogen status: transcriptomic identification of genes affected by a new signalling pathway.

    Science.gov (United States)

    Omrane, Selim; Ferrarini, Alberto; D'Apuzzo, Enrica; Rogato, Alessandra; Delledonne, Massimo; Chiurazzi, Maurizio

    2009-01-01

    In leguminous plants, symbiotic nitrogen (N) fixation performances and N environmental conditions are linked because nodule initiation, development and functioning are greatly influenced by the amount of available N sources. We demonstrate here that N supply also controls, beforehand, the competence of leguminous plants to perform the nodulation program. Lotus japonicus plants preincubated for 10 d in high-N conditions, and then transferred to low N before the Mesorhizobium loti inoculation, had reduced nodulation. This phenotype was maintained for at least 6 d and a complete reacquisition of the symbiotic competence was observed only after 9 d. The time-course analysis of the change of the symbiotic phenotype was analysed by transcriptomics. The differentially expressed genes identified are mostly involved in metabolic pathways. However, the transcriptional response also includes genes belonging to other functional categories such as signalling, stress response and transcriptional regulation. Some of these genes show a molecular identity and a regulation profile, that suggest a role as possible molecular links between the N-dependent plant response and the nodule organogenesis program.

  20. A superparamagnetic Fe3O4-graphene oxide nanocomposite for enrichment of nuciferine in the extract of Nelumbinis Folium (Lotus leaf)

    Science.gov (United States)

    Fan, Jie-Ping; Zheng, Bing; Qin, Yu; Yang, Dan; Liao, Dan-Dan; Xu, Xiao-Kang; Zhang, Xue-Hong; Zhu, Jian-Hang

    2016-02-01

    In this work, a superparamagnetic Fe3O4-graphene oxide (MGO) nanocomposite was prepared by one-step chemical co-precipitation method, and characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), powder X-ray diffraction (PXRD), Raman spectroscopy and nitrogen adsorption-desorption curve. The as-prepared MGO was used to adsorb nuciferine, and the adsorption kinetic, isotherm and reusability of MGO were also investigated. The results showed that the adsorption of nuciferine on MGO reached its equilibrium very quickly (within 10 min) due to the two-dimensional carbon nanostructure of GO. In comparison with MGO, five conventional sorbents, i.e., macroporous resin D-101, silica gel, reverse phase silica gel (RP-C18) and cation exchange resin and polyamide, were also used to evaluate their adsorption capabilities. Therefore, MGO combined the advantages of both superparamagnetic particle and GO, i.e., easy separation and high absorption capacity. Finally, MGO was successfully applied to enrichment and separation of nuciferine in the extract of Nelumbinis Folium (Lotus leaf).

  1. Lotus japonicus plants of the Gifu B-129 ecotype subjected to alkaline stress improve their Fe(2+) bio-availability through inoculation with Pantoea eucalypti M91.

    Science.gov (United States)

    Campestre, María Paula; Castagno, Luis Nazareno; Estrella, María Julia; Ruiz, Oscar Adolfo

    2016-03-15

    Inoculation assays with Pantoea eucalypti M91 were performed on Lotus japonicus ecotype Gifu. Under alkaline conditions, this ecotype is characterized by the development of interveinal chlorosis of the apical leaves due to low mobilization of Fe(2+). Inoculation with P. eucalypti M91, a plant growth-promoting bacterial strain capable of producing pyoverdine-like and pyochelin-like siderophores under alkaline growth conditions, alters the root, resulting in a herringbone pattern of root branching. Additional features include improvement in Fe(2+) transport to the shoots, acidification of the hydroponic solution of the plant cultures, and an accompanying increase in the efficiency of the PSII parameters. In addition, there was an increase in the expression of the FRO1 and IRT1 genes, accompanied by a significant increase in FRO activity. Results showed that P. eucalypti M91 has a beneficial effect on the Fe acquisition machinery of Strategy I, as described for non-graminaceous monocots and dicots, suggesting its potential as an inoculant for legume crops cultivated in alkaline soils. Copyright © 2016 Elsevier GmbH. All rights reserved.

  2. Interfacial film stabilized W/O/W nano multiple emulsions loaded with green tea and lotus extracts: systematic characterization of physicochemical properties and shelf-storage stability.

    Science.gov (United States)

    Mahmood, Tariq; Akhtar, Naveed; Manickam, Sivakumar

    2014-05-12

    Multiple emulsions have excellent encapsulating potential and this investigation has been aimed to encapsulate two different plant extracts as functional cosmetic agents in the W/O/W multiple emulsions and the resultant system's long term stability has been determined in the presence of a thickener, hydroxypropyl methylcellulose (HPMC). Multiple W/O/W emulsions have been generated using cetyl dimethicone copolyol as lipophilic emulsifier and a blend of polyoxyethylene (20) cetyl ether and cetomacrogol 1000® as hydrophilic emulsifiers. The generated multiple emulsions have been characterized with conductivity, pH, microscopic analysis, phase separation and rheology for a period of 30 days. Moreover, long term shelf-storage stability has been tested to understand the shelf-life by keeping the generated multiple emulsion formulations at 25 ± 10°C and at 40 ± 10% relative humidity for a period of 12 months. It has been observed that the hydrophilic emulsifiers and HPMC have considerably improved the stability of multiple emulsions for the followed period of 12 months at different storage conditions. These multiple emulsions have shown improved entrapment efficiencies concluded on the release rate of conductometric tracer entrapped in the inner aqueous phase of the multiple emulsions. Multiple emulsions have been found to be stable for a longer period of time with promising characteristics. Hence, stable multiple emulsions loaded with green tea and lotus extracts could be explored for their cosmetic benefits.

  3. Relationships between phenotypic variation in osmotic adjustment, water-use efficiency, and drought tolerance of seven cultivars of Lotus corniculatus L.

    Directory of Open Access Journals (Sweden)

    Luis Inostroza

    2015-03-01

    Full Text Available Lotus corniculatus L. is a perennial forage legume species highly-adapted to growth under drought conditions. However, the genetic and physiological mechanisms involved in its adaptive capacity have not been elucidated. The role of osmotic adjustment (OA and water-use efficiency (WUE on the drought tolerance of L. corniculatus was studied in a greenhouse experiment. Seven cultivars of different origin were subjected to two contrasting treatments of available soil water: No water stress (NWS and with water stress (WWS. Xylem water potential (Ψx, osmotic potential (Ψπ, pressure potential (Ψp, relative water content (RWC, stomatal conductance (g s, shoot DM production, water transpiration (T, and WUE (shoot DM/T were measured. Water treatments significantly (P < 0.05 affected plant water status, which was reflected in reduced Ψx, RWC, g s, and transpiration rate in the WWS treatment compared with the NWS treatment. All cultivars showed a high capacity for OA under WWS treatment because Ψπ decreased by approximately 60% and Ψp increased by approximately 30%, compared with the NWS treatment. Cultivars with a higher solute accumulation (low Ψπ value had the lowest DM production under WWS treatment. In contrast, WUE varied greatly among cultivars and was positively associated (R² = 0.88; P < 0.01 with DM production under drought conditions.

  4. Is Continuing Contumely Relative to Mc Leod's Vision and ``Secret Sacred Science, (SSS),'': Contagiously Counterproductive in Science, or an Unhealthy Artifact of ``Turf Wars''?

    Science.gov (United States)

    Leod, Roger

    2007-04-01

    Mc Leod confirmed, with physics, his models for vision, and for electromagnetic artifacts, by traditional methods, associated with phenomena like tornados, hurricanes, and earthquakes. The latter confirmations are evidently apparent across current ethnology, cultures, linguistics, religion, rituals, exotic astronomy, somewhat concealed evidence of native record-keeping/writing, and iconography. Use of cultural anthropology while observing a modern Peruvian sacred-site-sweeping at Cuzco, coupled with their assertion that Ñari Huallac means ``serpent God,'' plus electromagnet information, reveals that their religious world-view include(s)(d) applied science that is still otherwise unacknowledged. Alexander Thom's precise megalithic site-measurements also imply that ``The Ancients' Serpent'' made/makes precise tracks that convey valuable information. The linguistics of words like Seminole, and unusual visual effects, reveal some traditionalists have done better than most scientists, for vision, and observational physics, and earth science. Tornado and hurricane tracks are predictable, as are some earthquakes. Tornado ``detuning'' or shutdown is electromagnetically possible. To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2007.NES07.C2.7

  5. Duncan Tanner Essay Prize Winner 2014. Against the 'Sacred Cow': NHS Opposition and the Fellowship for Freedom in Medicine, 1948-72.

    Science.gov (United States)

    Seaton, Andrew

    2015-01-01

    This essay recovers organized opposition to the National Health Service (NHS) by considering the Fellowship for Freedom in Medicine (FFM), a conservative organization of doctors who challenged the 'Sacred Cow' of nationalized healthcare in the 1950s and 1960s. While there has been little interest in anti-NHS politics because of shortcomings in the institution's historiography, this study suggests ways a new history of the service can be written. Central to that project is taking the broader ideological and emotive quality of the NHS seriously, and appreciating the way, for all sides of the political spectrum, as well as the general public, the service has always been a contested symbol of post-war British identity. This essay argues that two NHS 'crises'--panics over costs, and disillusionment within general practice--were not merely disagreements over budgets and pay-packets but politically charged moments infused with conservative anxieties over Britain's post-war trajectory. The FFM imagined the NHS as an economically dangerous bureaucratic machine that crushed medical independence and risked pushing the country towards dictatorship. Allies within the Conservative Party, private health insurance industry, and free-market 'think-tanks' worked with the FFM to challenge defences of both the service's operation and meaning. To appreciate why the NHS remains 'the closest thing the English have to a religion', one must consider the apostates as well as the faithful.

  6. Comparing laparoscopic antireflux surgery with esomeprazole in the management of patients with chronic gastro-oesophageal reflux disease: a 3-year interim analysis of the LOTUS trial

    Science.gov (United States)

    Lundell, L; Attwood, S; Ell, C; Fiocca, R; Galmiche, J-P; Hatlebakk, J; Lind, T; Junghard, O

    2008-01-01

    Background: With the introduction of laparoscopic antireflux surgery (LARS) for gastro-oesophageal reflux disease (GORD) along with the increasing efficacy of modern medical treatment, a direct comparison is warranted. The 3-year interim results of a randomised study comparing both the efficacy and safety of LARS and esomeprazole (ESO) are reported. Methods: LOTUS is an open, parallel-group multicentre, randomised and controlled trial conducted in dedicated centres in 11 European countries. LARS was completed according to a standardised protocol, comprising a total fundoplication and a crural repair. Medical treatment comprised ESO 20 mg once daily, which could be increased stepwise to 40 mg once daily and then 20 mg twice daily in the case of incomplete GORD control. The primary outcome variable was time to treatment failure (Kaplan–Meier analysis). Treatment failure was defined on the basis of symptomatic relapse requiring treatment beyond that stated in the protocol. Results: 554 patients were randomised, of whom 288 were allocated to LARS and 266 to ESO. The two study arms were well matched. The proportions of patients who remained in remission after 3 years were similar for the two therapies: 90% of surgical patients compared with 93% medically treated for the intention to treat population, p = 0.25 (90% vs 95% per protocol). No major unexpected postoperative complications were experienced and ESO was well tolerated. However, postfundoplication complaints remain a problem after LARS. Conclusions: Over the first 3 years of this long-term study, both laparoscopic total fundoplication and continuous ESO treatment were similarly effective and well-tolerated therapeutic strategies for providing effective control of GORD. PMID:18469091

  7. Long-term effect on symptoms and quality of life of maintenance therapy with esomeprazole 20 mg daily: a post hoc analysis of the LOTUS trial.

    Science.gov (United States)

    Lundell, Lars; Hatlebakk, Jan; Galmiche, Jean-Paul; Attwood, Stephen E; Ell, Christian; Fiocca, Roberto; Persson, Tore; Nagy, Péter; Eklund, Stefan; Lind, Tore

    2015-01-01

    To assess the long-term effect on symptoms and quality of life of esomeprazole 20 mg once daily, a recommended dose for maintenance therapy of gastroesophageal reflux disease (GERD). This is a post hoc analysis of 5 year data from patients in the LOTUS trial (ClinicalTrials.gov identifier: NCT00251927) who were randomized to esomeprazole 20 mg once daily. All participants had chronic, symptomatic GERD responsive to treatment. Gastrointestinal symptoms were assessed by physicians and by using patient-reported outcome instruments. Investigations included gastrointestinal endoscopy (with biopsy sampling), 24 hour esophageal pH monitoring and laboratory measurements. In total, 157 of 256 patients randomized to esomeprazole 20 mg once daily remained on this dose until the end of follow-up or study discontinuation, whereas 99 patients had their dose increased because of inadequate symptom control (of these, 29 subsequently returned to the allocated dose). On logistic regression, a long objectively defined GERD history, smoking, female sex, absence of Helicobacter pylori infection and high supine baseline acid reflux into the esophagus were associated with an increased likelihood of requiring dose escalation to esomeprazole 40 mg daily (all p esomeprazole 20 mg once daily, with no more than mild symptom severity, and mean (standard deviation) percentage time with intraesophageal pH Esomeprazole at a maintenance dose of 20 mg once daily offers effective long-term treatment for chronic GERD in patients initially responsive to the medication, with durable symptom control and sustained reductions in intraesophageal acid exposure.

  8. Hydrocarbon degradation potential and plant growth-promoting activity of culturable endophytic bacteria of Lotus corniculatus and Oenothera biennis from a long-term polluted site.

    Science.gov (United States)

    Pawlik, Małgorzata; Cania, Barbara; Thijs, Sofie; Vangronsveld, Jaco; Piotrowska-Seget, Zofia

    2017-08-01

    Many endophytic bacteria exert beneficial effects on their host, but still little is known about the bacteria associated with plants growing in areas heavily polluted by hydrocarbons. The aim of the study was characterization of culturable hydrocarbon-degrading endophytic bacteria associated with Lotus corniculatus L. and Oenothera biennis L. collected in long-term petroleum hydrocarbon-polluted site using culture-dependent and molecular approaches. A total of 26 hydrocarbon-degrading endophytes from these plants were isolated. Phylogenetic analyses classified the isolates into the phyla Proteobacteria and Actinobacteria. The majority of strains belonged to the genera Rhizobium, Pseudomonas, Stenotrophomonas, and Rhodococcus. More than 90% of the isolates could grow on medium with diesel oil, approximately 20% could use n-hexadecane as a sole carbon and energy source. PCR analysis revealed that 40% of the isolates possessed the P450 gene encoding for cytochrome P450-type alkane hydroxylase (CYP153). In in vitro tests, all endophytic strains demonstrated a wide range of plant growth-promoting traits such as production of indole-3-acetic acid, hydrogen cyanide, siderophores, and phosphate solubilization. More than 40% of the bacteria carried the gene encoding for the 1-aminocyclopropane-1-carboxylic acid deaminase (acdS). Our study shows that the diversity of endophytic bacterial communities in tested plants was different. The results revealed also that the investigated plants were colonized by endophytic bacteria possessing plant growth-promoting features and a clear potential to degrade hydrocarbons. The properties of isolated endophytes indicate that they have the high potential to improve phytoremediation of petroleum hydrocarbon-polluted soils.

  9. Anion inhibition studies of two α-carbonic anhydrases from Lotus japonicus, LjCAA1 and LjCAA2.

    Science.gov (United States)

    Vullo, Daniela; Flemetakis, Emmanouil; Scozzafava, Andrea; Capasso, Clemente; Supuran, Claudiu T

    2014-07-01

    The model organism for the investigation of symbiotic nitrogen fixation in legumes Lotus japonicus encodes two carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the α-class, LjCAA1 and LjCAA2. Here we report the kinetic characterization and inhibition of these two CAs with inorganic and complex anions and other molecules interacting with zinc proteins, such as sulfamide, sulfamic acid, and phenylboronic/arsonic acids. LjCAA1 showed a high catalytic activity for the CO2 hydration reaction, with a k(cat) of 7.4∗10(5) s(-1) and a k(cat)/K(m) of 9.6∗10(7) M(-1) s(-1) and was inhibited in the low micromolar range by N,N-diethyldithiocarbamate, sulfamide, sulfamic acid, phenylboronic/arsonic acid (K(I)s of 4-62 μM). LjCAA2 showed a moderate catalytic activity for the physiologic reaction, with a k(cat) of 4.0∗10(5) s(-1) and a k(cat)/K(m) of 4.9∗10(7) M(-1) s(-1). The same anions mentioned above for the inhibition of LjCAA1 showed the best activity against LjCAA2 (K(I)s of 7-29 μM). Nitrate and nitrite, anions involved in nitrogen fixation, showed lower affinity for the two enzymes, with inhibition constants in the range of 3.7-7.0 mM. Halides and sulfate also behaved in a distinct manner towards the two enzymes investigated here. As LjCAA1/2 participate in the pH regulation processes and CO2 metabolism within the nitrogen-fixing nodules of the plant, our studies may shed some light regarding these complex biochemical processes. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana

    Directory of Open Access Journals (Sweden)

    Boycheva I

    2015-08-01

    Full Text Available Irina Boycheva,1 Valya Vassileva,2 Miglena Revalska,1 Grigor Zehirov,2 Anelia Iantcheva1 1Department of Functional Genetics Legumes, 2AgroBioInstitute, Department of Plant Stress Molecular Biology, Institute of Plant Physiology and Genetics, Sofia, Bulgaria Abstract: In eukaryotes, F-box proteins are one of the main components of the SCF complex that belongs to the family of ubiquitin E3 ligases, which catalyze protein ubiquitination and maintain the balance between protein synthesis and degradation. In the present study, we clarified the role and function of the gene encoding cyclin-like F-box protein from Medicago truncatula using transgenic plants of the model species M. truncatula, Lotus japonicas, and Arabidopsis thaliana generated by Agrobacterium-mediated transformation. Morphological and transcriptional analyses combined with flow cytometry and histochemistry demonstrated the participation of this protein in many aspects of plant growth and development, including processes of indirect somatic embryogenesis and symbiotic nodulation. The cyclin-like F-box gene showed expression in all plant organs and tissues comprised of actively dividing cells. The observed variations in root and hypocotyl growth, leaf and silique development, ploidy levels, and leaf parameters in the obtained transgenic lines demonstrated the effects of this gene on organ development. Furthermore, knockdown of cyclin-like F-box led to accumulation of higher levels of the G2/M transition-specific gene cyclin B1:1 (CYCB1:1, suggesting its possible role in cell cycle control. Together, the collected data suggest a similar role of the cyclin-like F-box protein in the three model species, providing evidence for the functional conservation of the studied gene. Keywords: cyclin-like F-box, model legumes, Arabidopsis thaliana, plant growth, plant development, cell cycle

  11. Genome-wide analysis of AP2/ERF family genes from Lotus corniculatus shows LcERF054 enhances salt tolerance.

    Science.gov (United States)

    Sun, Zhan-Min; Zhou, Mei-Liang; Xiao, Xing-Guo; Tang, Yi-Xiong; Wu, Yan-Min

    2014-09-01

    Lotus corniculatus is used in agriculture as a main forage plant. Members of the Apetala2/ethylene response factor (AP2/ERF) family play important roles in regulating gene expression in response to many forms of stress, including drought and salt. Here, starting from database of the L. corniculatus var. japonicus genome, we identified 127 AP2/ERF genes by insilico cloning method. The phylogeny, gene structures, and putative conserved motifs in L. corniculatus var. japonicus ERF proteins were analyzed. Based on the number of AP2/ERF domains and the function of the genes, 127 AP2/ERF genes from L. corniculatus var. japonicus were classified into five subfamilies named the AP2, dehydration-responsive element binding factor (DREB), ERF, RAV, and a soloist. Outside the AP2/ERF domain, many L. corniculatus var. japonicus-specific conserved motifs were detected. Expression profile analysis of AP2/ERF genes by quantitative real-time PCR revealed that 19 LcERF genes, including LcERF054 (KJ004728), were significantly induced by salt stress. The results showed that the LcERF054 gene encodes a nuclear transcription activator. Overexpression of LcERF054 in Arabidopsis enhanced the tolerances to salt stress, showed higher germination ratio of seeds, and had elevated levels of relative moisture contents, soluble sugars, proline, and lower levels of malondialdehyde under stress conditions compared to wild-type plants. The expression of hyperosmotic salinity response genes COR15A, LEA4-5, P5CS1, and RD29A was found to be elevated in the LcERF054-overexpressing Arabidopsis plants compared to wild type. These results revealed that the LcERF genes play important roles in L. corniculatus cv Leo under salt stress and that LcERFs are attractive engineering targets in applied efforts to improve abiotic stress tolerances in L. corniculatus cv Leo or other crops.

  12. Heat stress results in loss of chloroplast Cu/Zn superoxide dismutase and increased damage to Photosystem II in combined drought-heat stressed Lotus japonicus.

    Science.gov (United States)

    Sainz, Martha; Díaz, Pedro; Monza, Jorge; Borsani, Omar

    2010-09-01

    Drought and heat stress have been studied extensively in plants, but most reports involve analysis of response to only one of these stresses. Studies in which both stresses were studied in combination have less commonly been reported. We report the combined effect of drought and heat stress on Photosystem II (PSII) of Lotus japonicus cv. Gifu plants. Photochemistry of PSII was not affected by drought or heat stress alone, but the two stresses together decreased PSII activity as determined by fluorescence emission. Heat stress alone resulted in degradation of D1 and CP47 proteins, and D2 protein was also degraded by combined drought-heat stress. None of these proteins were degraded by drought stress alone. Drought alone induced accumulation of hydrogen peroxide but the drought-heat combination led to an increase in superoxide levels and a decrease in hydrogen peroxide levels. Furthermore, combined drought-heat stress was correlated with an increase in oxidative damage as determined by increased levels of thiobarbituric acid reactive substances. Heat also induced degradation of chloroplast Cu/Zn superoxide dismutase (SOD: EC 1.15.1.1) as shown by reduced protein levels and isozyme-specific SOD activity. Loss of Cu/Zn SOD and induction of catalase (CAT: EC 1.11.1.6) activity would explain the altered balance between hydrogen peroxide and superoxide in response to drought vs combined drought-heat stress. Degradation of PSII could thus be caused by the loss of components of chloroplast antioxidant defence systems and subsequent decreased function of PSII. A possible explanation for energy dissipation by L. japonicus under stress conditions is discussed.

  13. Imagining Exodus for Israel-Palestine: Reading the Secular and the Sacred, Diaspora and Homeland, in Edward Said and David Grossman

    Directory of Open Access Journals (Sweden)

    Anna Hartnell

    2005-03-01

    Full Text Available This paper takes as its starting point Edward Said's distinction between 'religious' and 'secular' modes of cultural affiliation. As these simultaneously diverging and converging modes also trammel the particular grounds of thinking that have been Said's natural target of criticism - Zionism - his work speaks particularly powerfully to the debate surrounding the religious genealogy of Jewish identity. This paper argues that Said's interventions on Zionism highlight as problematic the position whereby the 'Ingathering of the Exiles' is promoted as coexisting with a 'diasporic consciousness' nurtured by Judaism during exile; messianic hopes of religious Jews cannot be reconciled with physical return to the Promised Land; identity circumscribed by ethnicity and place cannot stand in as exemplary for the exiled, unsettled and ultimately homeless identity trumpeted by discourses of the 'post', as many contemporary theorists would have it. And yet through an exploration of the writings of David Grossman, whose construction of Jewish identity is envisaged through the regulating, competing and collaborating tropes of Zionism and Diaspora, I argue that this position is crucial for the elaboration of Israeli identity. I also argue that in fact there is room within Said's thinking both for the anti-essentialist elaboration of 'homeless' identities as well as 'the permission to narrate' an identity politics, and that his own distinction between the 'secular' and the 'religious' begins to disassemble. I explore this blurring of the sacred and the secular through the prism of Exodus - as both concept and narrative. This paper suggests that it is precisely Said's achievement to embody these tensions between religion and its other, divine providence and human agency, historical materialism and postmodernism, alienation and its perennially tempting opposite: home.

  14. Lactobacillus casei-01 facilitates the ameliorative effects of proanthocyanidins extracted from lotus seedpod on learning and memory impairment in scopolamine-induced amnesia mice.

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    Juan Xiao

    Full Text Available Learning and memory abilities are associated with alterations in gut function. The two-way proanthocyanidins-microbiota interaction in vivo enhances the physiological activities of proanthocyanidins and promotes the regulation of gut function. Proanthocyanidins extracted from lotus seedpod (LSPC have shown the memory-enhancing ability. However, there has been no literature about whether Lactobacillus casei-01 (LC enhances the ameliorative effects of LSPC on learning and memory abilities. In this study, learning and memory abilities of scopolamine-induced amnesia mice were evaluated by Y-maze test after 20-day administration of LC (10(9 cfu/kg body weight (BW, LSPC (low dose was 60 mg/kg BW (L-LSPC and high dose was 90 mg/kg BW (H-LSPC, or LSPC and LC combinations (L-LSPC+LC and H-LSPC+LC. Alterations in antioxidant defense ability and oxidative damage of brain, serum and colon, and brain cholinergic system were investigated as the possible mechanisms. As a result, the error times of H-LSPC+LC group were reduced by 41.59% and 68.75% relative to those of H-LSPC and LC groups respectively. LSPC and LC combinations ameliorated scopolamine-induced memory impairment by improving total antioxidant capacity (TAOC level, glutathione peroxidase (GSH-Px and total superoxide dismutase (T-SOD activities of brain, serum and colon, suppressing malondialdehyde (MDA level of brain, serum and colon, and inhibiting brain acetylcholinesterase (AchE, myeloperoxidase, total nitric oxide synthase and neural nitric oxide synthase (nNOS activities, and nNOS mRNA level. Moreover, LC facilitated the ameliorative effects of H-LSPC on GSH-Px activity of colon, TAOC level, GSH-Px activity and ratio of T-SOD to MDA of brain and serum, and the inhibitory effects of H-LSPC on serum MDA level, brain nNOS mRNA level and AchE activity. These results indicated that LC promoted the memory-enhancing effect of LSPC in scopolamine-induced amnesia mice.

  15. Lactobacillus casei-01 facilitates the ameliorative effects of proanthocyanidins extracted from lotus seedpod on learning and memory impairment in scopolamine-induced amnesia mice.

    Science.gov (United States)

    Xiao, Juan; Li, Shuyi; Sui, Yong; Wu, Qian; Li, Xiaopeng; Xie, Bijun; Zhang, Mingwei; Sun, Zhida

    2014-01-01

    Learning and memory abilities are associated with alterations in gut function. The two-way proanthocyanidins-microbiota interaction in vivo enhances the physiological activities of proanthocyanidins and promotes the regulation of gut function. Proanthocyanidins extracted from lotus seedpod (LSPC) have shown the memory-enhancing ability. However, there has been no literature about whether Lactobacillus casei-01 (LC) enhances the ameliorative effects of LSPC on learning and memory abilities. In this study, learning and memory abilities of scopolamine-induced amnesia mice were evaluated by Y-maze test after 20-day administration of LC (10(9) cfu/kg body weight (BW)), LSPC (low dose was 60 mg/kg BW (L-LSPC) and high dose was 90 mg/kg BW (H-LSPC)), or LSPC and LC combinations (L-LSPC+LC and H-LSPC+LC). Alterations in antioxidant defense ability and oxidative damage of brain, serum and colon, and brain cholinergic system were investigated as the possible mechanisms. As a result, the error times of H-LSPC+LC group were reduced by 41.59% and 68.75% relative to those of H-LSPC and LC groups respectively. LSPC and LC combinations ameliorated scopolamine-induced memory impairment by improving total antioxidant capacity (TAOC) level, glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) activities of brain, serum and colon, suppressing malondialdehyde (MDA) level of brain, serum and colon, and inhibiting brain acetylcholinesterase (AchE), myeloperoxidase, total nitric oxide synthase and neural nitric oxide synthase (nNOS) activities, and nNOS mRNA level. Moreover, LC facilitated the ameliorative effects of H-LSPC on GSH-Px activity of colon, TAOC level, GSH-Px activity and ratio of T-SOD to MDA of brain and serum, and the inhibitory effects of H-LSPC on serum MDA level, brain nNOS mRNA level and AchE activity. These results indicated that LC promoted the memory-enhancing effect of LSPC in scopolamine-induced amnesia mice.

  16. Lotus tenuis x L. corniculatus interspecific hybridization as a means to breed bloat-safe pastures and gain insight into the genetic control of proanthocyanidin biosynthesis in legumes.

    Science.gov (United States)

    Escaray, Francisco J; Passeri, Valentina; Babuin, Florencia M; Marco, Francisco; Carrasco, Pedro; Damiani, Francesco; Pieckenstain, Fernando L; Paolocci, Francesco; Ruiz, Oscar A

    2014-02-03

    Proanthocyanidins (PAs) are secondary metabolites that strongly affect plant quality traits. The concentration and the structure of these metabolites influence the palatability and nutritional value of forage legumes. Hence, modulating PAs in the leaves of forage legumes is of paramount relevance for forage breeders worldwide. The lack of genetic variation in the leaf PA trait within the most important forage species and the difficulties in engineering this pathway via the ectopic expression of regulatory genes, prompted us to pursue alternative strategies to enhance this trait in forage legumes of agronomic interest. The Lotus genus includes forage species which accumulate PAs in edible organs and can thus be used as potential donor parents in breeding programs. We recovered a wild, diploid and PA-rich population of L. corniculatus and crossed with L. tenuis. The former grows in an alkaline-salty area in Spain while the latter is a diploid species, grown extensively in South American pastures, which does not accumulate PAs in the herbage. The resulting interspecific hybrids displayed several traits of outstanding agronomic relevance such as rhizome production, PA levels in edible tissues sufficient to prevent ruminal bloating (around 5 mg of PAs/g DW), biomass production similar to the cultivated parent and potential for adaptability to marginal lands. We show that PA levels correlate with expression levels of the R2R3MYB transcription factor TT2 and, in turn, with those of the key structural genes of the epicatechin and catechin biosynthetic pathways leading to PA biosynthesis. The L. tenuis x L. corniculatus hybrids, reported herein, represent the first example of the introgression of the PA trait in forage legumes to levels known to provide nutritional and health benefits to ruminants. Apart from PAs, the hybrids have additional traits which may prove useful to breed forage legumes with increased persistence and adaptability to marginal conditions. Finally, our

  17. Macrophytes aquatiques dans les eaux "continentales" ivoiriennes

    OpenAIRE

    N'dah, E.; Arfi, Robert

    1996-01-01

    Inventory and socio-economic impacts of water weed are presented. Free-floating species (Eichhornia crassipes, Pistia strationes) floating leaves plants (Nymphaea lotus, Polygonum senegalense) as well as emerged species such as Echinochloa pyramidalis and Nelumbo nucifera are the most common species in the concerned aquatic environments. These plants have been colonizing these environments for about a decade and the colonized area are equivalent to 70 % of the whole of the water surface. Besi...

  18. Propagation and Establishment of Native Plants for Vegetative Restoration of Aquatic Ecosystems

    Science.gov (United States)

    2013-06-01

    constitute an official endorsement or approval of the use of such commercial products. All product names and trademarks cited are the property of their...Vallisneria americana submersed Yes No American lotus** Nelumbo lutea floating-leaved No Yes Blue water lily Nymphaea elegans floating-leaved No Yes...trials (with and without fertilizer amendments , see below) to ascertain suitability for supporting aquatic plant growth. Because floating- leaved

  19. The Sacred in Contemporary Advertising

    Directory of Open Access Journals (Sweden)

    Cătălin Soreanu

    2016-05-01

    Full Text Available As part of the contemporary media culture, advertising constantly mediates the relationship between the institutional dimension of religion (namely the church and the community, given the specific position of the Catholic religion: transparent, based on dialogue and the continuous questioning of the relationship between man and divinity. To manage their presence in public life, the church constantly approaches new media and specific forms of communication (posters, street banners and meshes, websites or interactive mobile structures.

  20. Nodulação e fixação biológica de nitrogênio de Adesmia latifolia e Lotus corniculatus em vasos de Leonard Nodulation and biological nitrogen fixation of Adesmia latifolia and Lotus corniculatus in Leonard jars

    Directory of Open Access Journals (Sweden)

    Simone Meredith Scheffer-Basso

    2001-06-01

    Full Text Available Adesmia latifolia, que é uma leguminosa forrageira nativa do Sul do Brasil, destaca-se pelo seu hábito de crescimento estolonífero e por produzir forragem durante a estação fria. O Lotus corniculatus (cornichão é uma forrageira comercialmente utilizada na formação de pastagens hibernais em regiões subtropicais e temperadas. Para ambas as espécies, no entanto, são limitados os trabalhos científicos relacionados ao padrão de nodulação e à fixação biológica de nitrogênio (FBN. Este trabalho foi desenvolvido com o objetivo de analisar tais processos nessas espécies, bem como comparar o seu desenvolvimento morfológico sob diferentes fontes de N: N-mineral (nitrato de amônio 5%, N-simbiótico (inoculação e na ausência dessas fontes (testemunha. O ensaio foi conduzido em casa de vegetação, utilizando-se vasos de Leonard com solução nutritiva; o substrato constou de uma mistura de areia + vermiculita + carvão. O delineamento foi completamente casualizado, com quatro repetições. Ao final de 65 dias, as plantas foram colhidas e avaliadas quanto ao comprimento e volume de raízes, número e peso de nódulos, acúmulo de MS e FBN. A. latifolia destacou-se pelo maior número de nódulos (126/vaso e peso total de nódulos (82,22 mg MS/vaso em relação ao cornichão, com 82 nódulos/vaso e 20,25 mg MS/vaso. A FBN foi mais efetiva em A. latifolia, cujas plantas inoculadas produziram, em média, 37% de MS em relação às plantas supridas com N-mineral; no cornichão esse percentual foi de apenas 15%. A quantidade de N fixado simbioticamente foi de 43,12 mg/vaso em Adesmia e de 9,92 mg N/vaso em cornichão.Adesmia latifolia is a forage legume native from Southern Brazil which is outstanding due to stoloniferous growth habit and for forage production during the cool season. The Lotus corniculatus (birdsfoot trefoil is a forage usually utilized as cool season species in subtropical and temperate regions. For both species, however