Debnath, Mithu; Valerio Iungo, G.; Ashton, Ryan; Brewer, W. Alan; Choukulkar, Aditya; Delgado, Ruben; Lundquist, Julie K.; Shaw, William J.; Wilczak, James M.; Wolfe, Daniel
Vertical profiles of 3-D wind velocity are retrieved from triple range-height-indicator (RHI) scans performed with multiple simultaneous scanning Doppler wind lidars. This test is part of the eXperimental Planetary boundary layer Instrumentation Assessment (XPIA) campaign carried out at the Boulder Atmospheric Observatory. The three wind velocity components are retrieved and then compared with the data acquired through various profiling wind lidars and high-frequency wind data obtained from sonic anemometers installed on a 300 m meteorological tower. The results show that the magnitude of the horizontal wind velocity and the wind direction obtained from the triple RHI scans are generally retrieved with good accuracy. However, poor accuracy is obtained for the evaluation of the vertical velocity, which is mainly due to its typically smaller magnitude and to the error propagation connected with the data retrieval procedure and accuracy in the experimental setup.
Lindelöw, Per Jonas Petter; Mohr, Johan Jacob
Range unambiguous high duty cycle coherent lidars can be constructed based on frequency stepped pulse train modulation, even continuously emitting systems could be envisioned. Such systems are suitable for velocity sensing of dispersed targets, like the atmosphere, at fast acquisition rates...
Schultz, Christopher J.; Carey, Lawerence D.; Brunning, Eric C.; Blakeslee, Richard
Four electrified snowfall cases are examined using total lightning measurements from lightning mapping arrays (LMAs), and the National Lightning Detection Network (NLDN) from Huntsville, AL and Washington D.C. In each of these events, electrical activity was in conjunction with heavy snowfall rates, sometimes exceeding 5-8 cm hr-1. A combination of LMA, and NLDN data also indicate that many of these flashes initiated from tall communications towers and traveled over large horizontal distances. During events near Huntsville, AL, the Advanced Radar for Meteorological and Operational Research (ARMOR) C-band polarimetric radar was collecting range height indicators (RHIs) through regions of heavy snowfall. The combination of ARMOR polarimetric radar and VHF LMA observations suggested contiguous layer changes in height between sloping aggregate-dominated layers and horizontally-oriented crystals. These layers may have provided ideal conditions for the development of extensive regions of charge and resultant horizontal propagation of the lightning flashes over large distances.
Chen, Chao; Wang, Zhangjun; Meng, Xiangqian; Qu, Junle; Du, Libin; Li, Xianxin; Lv, Bin; Kabanov, V. V.
A scanning micro-pulse lidar (MPL) was developed by Institute of Oceanographic Instrumentation, Shandong Academy of Sciences, which can be used for routine observations of optical properties, temporal and spatial variation of atmospheric aerosol and cloud in the lower troposphere. In addition to the optical system design, the design of 3 dimensional (3-D) scanning system controlled by servo motors is analyzed, including servo motor selection and mechanical design. Through the measurements in Qingdao, it is proved that 3-D scanning system can control the lidar azimuth/elevation scanning with high precision. The lidar has good performance and can provide time-height indication (THI), range-height indication (RHI) and plane-position indication (PPI) of lidar signals which can well reflect the temporal and spatial variation of atmospheric aerosol.
Full Text Available Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT, HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063 National Marrow Donor Program (NMDP registry donors using long-range PCR by next generation sequencing (NGS approach on buccal swab DNA.Multiplex long-range PCR primers amplified the full-length of HLA class I genes (A, B, C from promotor to 3' UTR. Class II genes (DRB1, DQB1 were amplified from exon 2 through part of exon 4. PCR amplicons were pooled and sheared using Covaris fragmentation. Library preparation was performed using the Illumina TruSeq Nano kit on the Beckman FX automated platform. Each sample was tagged with a unique barcode, followed by 2×250 bp paired-end sequencing on the Illumina MiSeq. HLA typing was assigned using Omixon Twin software that combines two independent computational algorithms to ensure high confidence in allele calling. Consensus sequence and typing results were reported in Histoimmunogenetics Markup Language (HML format. All homozygous alleles were confirmed by Luminex SSO typing and exon novelties were confirmed by Sanger sequencing.Using this automated workflow, over 10,063 NMDP registry donors were successfully typed under high-resolution by NGS. Despite known challenges of nucleic acid degradation and low DNA concentration commonly associated with buccal-based specimens, 97.8% of samples were successfully amplified using long-range PCR. Among these, 98.2% were successfully reported by NGS, with an accuracy rate of 99.84% in an independent blind Quality Control audit performed by the NDMP. In this study, NGS-HLA typing identified 23 null alleles (0.023%, 92 rare alleles (0.091% and 42 exon novelties (0.042%.Long-range
Yin, Yuxin; Lan, James H; Nguyen, David; Valenzuela, Nicole; Takemura, Ping; Bolon, Yung-Tsi; Springer, Brianna; Saito, Katsuyuki; Zheng, Ying; Hague, Tim; Pasztor, Agnes; Horvath, Gyorgy; Rigo, Krisztina; Reed, Elaine F; Zhang, Qiuheng
Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT), HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS) of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063 National Marrow Donor Program (NMDP) registry donors using long-range PCR by next generation sequencing (NGS) approach on buccal swab DNA. Multiplex long-range PCR primers amplified the full-length of HLA class I genes (A, B, C) from promotor to 3' UTR. Class II genes (DRB1, DQB1) were amplified from exon 2 through part of exon 4. PCR amplicons were pooled and sheared using Covaris fragmentation. Library preparation was performed using the Illumina TruSeq Nano kit on the Beckman FX automated platform. Each sample was tagged with a unique barcode, followed by 2×250 bp paired-end sequencing on the Illumina MiSeq. HLA typing was assigned using Omixon Twin software that combines two independent computational algorithms to ensure high confidence in allele calling. Consensus sequence and typing results were reported in Histoimmunogenetics Markup Language (HML) format. All homozygous alleles were confirmed by Luminex SSO typing and exon novelties were confirmed by Sanger sequencing. Using this automated workflow, over 10,063 NMDP registry donors were successfully typed under high-resolution by NGS. Despite known challenges of nucleic acid degradation and low DNA concentration commonly associated with buccal-based specimens, 97.8% of samples were successfully amplified using long-range PCR. Among these, 98.2% were successfully reported by NGS, with an accuracy rate of 99.84% in an independent blind Quality Control audit performed by the NDMP. In this study, NGS-HLA typing identified 23 null alleles (0.023%), 92 rare alleles (0.091%) and 42 exon novelties (0.042%). Long-range
Suzuki, Kenji; Nakagawa, Katsuhiro; Kawano, Tetsuya; Mori, Shuichi; Katsumata, Masaki; Yoneyama, Kunio
During November-December 2015, as a pilot study of the Years of the Maritime and Continent (YMC), a campaign observation over the southwestern coastal land and adjacent sea of Sumatera Island, Indonesia was carried out to examine land-ocean coupling processes in mechanisms of coastal heavy rain. Our videosonde observations were conducted as a part of this campaign for the better understandings of microphysical features in tropical precipitating clouds developed over the Sumatera Island. Videosonde is one of strong tools to measure hydrometeors in clouds directly. It is a balloon-borne radiosonde that acquires images of precipitation particles via a CCD camera. The system has a stroboscopic illumination that provides information on particle size and shape. One of the advantages for the videosonde is to capture images of precipitation particles as they are in the air because the videosonde can obtain particle images without contact. Recorded precipitation particles are classified as raindrops, frozen drops (hail), graupel, ice crystals, or snowflakes on the basis of transparency and shape. Videosondes were launched from BMKG Bengkulu weather station (3.86°S，102.3°E). After the launch of a videosonde, the Range Height Indicator (RHI) scans by a C-band dual-polarimetric radar installed on R/V Mirai, which was approximately 50 km off Sumatera Island, were continuously performed, targeting the videosonde in the precipitating cloud. Eighteen videosondes were launched into various types of tropical precipitating clouds during the Pre-YMC campaign.
Main, Katharina M; Sehested, Astrid; Feldt-Rasmussen, Ulla
with acromegaly. We wanted to investigate whether pegvisomant was effective in a child with octreotide-resistant GH excess. CASE: A 4-year-old girl with neurofibromatosis type 1 and GH excess associated with optic glioma received pegvisomant injections (10 mg subcutaneously) with increasing intervals from daily...... to every 4th day. RESULTS: IGF-I and IGFBP-3 decreased from +6.9 and 4.6 standard deviation scores (SDS), respectively, to within normal range. Height velocity dropped from 12.4 SDS to mean -0.7 SDS (range: -5.0 to 5.0) and height SDS decreased from +1.3 to +0.6 (target height: +0.2). Random non......-fasting serum GH values were mean 5.0 mlU/l (range: 1.6-9.5). There was no change in fasting blood glucose (4.6-4.7 mmol/l) or glycosylated haemoglobin (5.5%) and no subjective or biochemical side effects. Repeated tests of thyroid, adrenal and gonadal function showed no alterations during the treatment period...
Lamer, K.; Tatarevic, A.; Jo, I.; Kollias, P.
The scanning Atmospheric Radiation Measurement (ARM) cloud radars (SACRs) provide continuous atmospheric observations aspiring to capture the 3-D cloud-scale structure. Sampling clouds in 3-D is challenging due to their temporal-spatial scales, the need to sample the sky at high elevations and cloud radar limitations. Thus, a suggested scan strategy is to repetitively slice the atmosphere from horizon to horizon as clouds advect over the radar (Cross-Wind Range-Height Indicator - CW-RHI). Here, the processing and gridding of the SACR CW-RHI scans are presented. First, the SACR sample observations from the ARM Southern Great Plains and Cape Cod sites are post-processed (detection mask, gaseous attenuation correction, insect filtering and velocity de-aliasing). The resulting radial Doppler moment fields are then mapped to Cartesian coordinates with time as one of the dimensions. Next the Cartesian-gridded Doppler velocity fields are decomposed into the horizontal wind velocity contribution and the vertical Doppler velocity component. For validation purposes, all gridded and retrieved fields are compared to collocated zenith-pointing ARM cloud radar measurements. We consider that the SACR sensitivity loss with range, the cloud type observed and the research purpose should be considered in determining the gridded domain size. Our results also demonstrate that the gridded SACR observations resolve the main features of low and high stratiform clouds. It is established that the CW-RHI observations complemented with processing techniques could lead to robust 3-D cloud dynamical representations up to 25-30 degrees off zenith. The proposed gridded products are expected to advance our understanding of 3-D cloud morphology, dynamics and anisotropy and lead to more realistic 3-D radiative transfer calculations.
Hyland, P.; Biggerstaff, M. I.; Uman, M. A.; Hill, J. D.; Krehbiel, P. R.; Rison, W.
During the summers of 2011-2012, a C-band polarimetric Shared Mobile Atmospheric Research and Teaching (SMART) radar from the University of Oklahoma was deployed to Keystone Heights, FL to study the relationship between cloud structure and the propagation of triggered and natural lightning channels. The radar was operated in Range-Height-Indicator (RHI) volume scanning mode over a narrow azimuthal sector that provided high spatial vertical resolution every 90 seconds over the rocket launch facility at the International Center for Lightning Research and Testing (ICLRT) at Camp Blanding, FL. In this presentation, we will focus on observations collected in 2011. Seven successful triggers (with return strokes) out of 20 attempts were sampled by the SMART-R from June to August. Most of the trigger attempts occurred during the dissipating stages of convection with steady ground electric field values. Specific differential phase (KDP) showed evidence of ice crystal alignment due to strong electric fields within the upper portions of the convection over ICLRT around the time of launch attempts. Consecutive RHI sweeps over ICLRT revealed changes in KDP that suggested the building of electric fields and subsequent relaxation after a triggered flash. KDP signatures relative to other radar variables will also be investigated to determine the microphysical and convective nature of the storms in which natural and triggered lightning strikes occurred. Lightning Mapping Array (LMA) sources of the triggered flash channels showed a preference for horizontal propagation just above the radar bright band associated with the melting layer. This finding agrees with several past studies that used balloon soundings and found intense layers of charge near the 0°C isotherm. The propagation path also seemed to be related to the vertical distribution of KDP in some of the triggered flashes. A preferred path through areas of generally positive values of KDP suggests that triggered lightning
Wulfmeyer, V.; Turner, D. D.; Mauder, M.; Behrendt, A.; Ingwersen, J.; Streck, T.
Improved simulations of land-surface-atmosphere interaction are fundamental for improving weather forecast and climate models. This requires observations of 2D fields of surface fluxes and the 3D structure of the atmospheric boundary layer simultaneously. A novel strategy is introduced for studying land-surface exchange and entrainment processes in the convective boundary layer (CBL) over complex terrain by means of a new generation of remote sensing systems. The sensor synergy consists of scanning Doppler lidar (DL), water-vapor differential absorption lidar (WVDIAL), and temperature rotational Raman lidar (TRRL) systems supported by surface in-situ measurements. The 2D measurements of surface fluxes are realized by the operation of a DL, a WVDIAL, and a TRRL along the same line-of-sight (LOS) in a range-height-indicator (RHI) mode whereas the other DL is performing a series of cross track RHI scans along this LOS. This new setup enables us to determine the friction velocity as well as surface sensible and latent heat fluxes by closing the complete set of Monin-Obukhov similarity relationships under a variety of surface layer stability conditions and different land cover and soil properties. As this closure is performed at all DL crossing points along the LOS, this is a strategy towards a 2D mapping of surface fluxes entirely based on remote sensing systems. Further details are presented at the conference. The second configuration is the simultaneous vertical profiling of vertical wind, humidity and temperature by DL, WVDIAL and TRRL so that latent heat and sensible heat flux profiles as well as a variety of different turbulent moments can be measured in the CBL. Consequently, by alternating of RHI scanning and vertical pointing modes, entrainment fluxes and surface fluxes can be measured almost simultaneously. This novel strategy has been realized for the first time during the Surface Atmospheric Boundary Layer Exchange (SABLE) campaign in the Kraichgau region
Wulfmeyer, Volker [University of Hohenheim; Turner, David [NOAA National Severe Storms Laboratory
The Land-Atmosphere Feedback Experiment (LAFE; pronounced “la-fey”) deploys several state-of-the-art scanning lidar and remote sensing systems to the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility’s Southern Great Plains (SGP) site. These instruments will augment the ARM instrument suite in order to collect a data set for studying feedback processes between the land surface and the atmosphere. The novel synergy of remote-sensing systems will be applied for simultaneous measurements of land-surface fluxes and horizontal and vertical transport processes in the atmospheric convective boundary layer (CBL). The impact of spatial inhomogeneities of the soil-vegetation continuum on land-surface-atmosphere (LSA) feedback will be studied using the scanning capability of the instrumentation. The time period of the observations is August 2017, because large differences in surface fluxes between different fields and bare soil can be observed, e.g., pastures versus fields where the wheat has already been harvested. The remote sensing system synergy will consist of three components: 1) The SGP water vapor and temperature Raman lidar (SRL), the SGP Doppler lidar (SDL), and the National Center for Atmospheric Research (NCAR) water vapor differential absorption lidar (DIAL) (NDIAL) mainly in vertical staring modes to measure mean profiles and gradients of moisture, temperature, and horizontal wind. They will also measure profiles of higher-order turbulent moments in the water vapor and wind fields and profiles of the latent heat flux. 2) A novel scanning lidar system synergy consisting of the National Oceanic and Atmospheric Administration (NOAA) High-Resolution Doppler lidar (HRDL), the University of Hohenheim (UHOH) water-vapor differential absorption lidar (UDIAL), and the UHOH temperature Raman lidar (URL). These systems will perform coordinated range-height indicator (RHI) scans from just above the canopy level to the
Shibata, Y.; Nagasawa, C.; Abo, M.
Knowledge of present carbon sources and sinks including their spatial distribution and their variation in time is one of the essential information for predicting future CO2 atmospheric concentration levels. Moreover, wind information is an important parameter for transport simulations and inverse estimation of surface CO2 flux. The differential absorption lidar (DIAL) and the Doppler wind lidar with the range resolution is expected to measure atmospheric CO2 profiles and wind profiles in the atmospheric boundary layer and lower troposphere from a ground platform. We have succeeded to develop a scanning 1.6 μm DIAL and incoherent Doppler lidar system for simultaneously measuring CO2 concentration and wind speed profiles. Our 1.6 μm DIAL system consists of the Optical Parametric Generator (OPG) transmitter that excited by the LD pumped Nd: YAG laser with high repetition rate (500 Hz) and the receiving optics that included the near-infrared photomultiplier tube with high quantum efficiency operating at the photon counting mode, a fiber Bragg grating (FBG) filter to detect a Doppler shift, and a 25 cm telescope  . We had developed an optical parametric oscillator (OPO) system for 1.6 μm CO2 DIAL. To achieve continuous tuning of the resonant OPO output without mode hopping, it is necessary to vary the OPO cavity length synchronously with the seed-frequency. On the other hand, the OPG does not require a cavity and instead rely on sufficient conversion efficiency to be obtained with a single pass through the crystal. The single-frequency oscillation of the OPG was achieved by injection seeding. The CO2-DIAL was operated with the range-height indicator (RHI) mode, and the 2-D measurement provided inhomogeneity in the boundary layer. Vertical CO2 concentration profiles and wind profiles were also measured simultaneously. The elevation angle was fixed at 52 deg and CO2 concentration profiles were obtained up to 1 km altitude with 200 m height resolution. Vertical