Lindelöw, Per Jonas Petter; Mohr, Johan Jacob
Range unambiguous high duty cycle coherent lidars can be constructed based on frequency stepped pulse train modulation, even continuously emitting systems could be envisioned. Such systems are suitable for velocity sensing of dispersed targets, like the atmosphere, at fast acquisition rates....... The lightwave synthesized frequency sweeper is a suitable generator yielding fast pulse repetition rates and stable equidistant frequency steps. Theoretical range resolution profiles of modulated lidars are presented....
National Aeronautics and Space Administration — The GPM Ground Validation Dual Polarized C-Band Doppler Radar King City GCPEx dataset has special Range Height Indicator (RHI) and sector scans of several dual...
SALIMI, Hamid; BARATI, Amir; ADIBPOUR, Nahid
The aim of this study was to compare an bodypump and resistance training on agility and vertical jump on male basketball players 16-18years. Therefore 36 teenage basketball players voluntarily chosen and randomly divided into three groups including: bodypump group (n=12,age 17 ± 0.5 year, height 179 ± 0.05 cm and a weight 69.26± 12.22 kg) and resistance group (n=12, age rangen 17± 0.6 years, range height 178 ± 0.09 cm and weight 68.55 ± 14.25 kg) and control group (n=12, age 17 ± 0.5 years, h...
Full Text Available Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT, HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063 National Marrow Donor Program (NMDP registry donors using long-range PCR by next generation sequencing (NGS approach on buccal swab DNA.Multiplex long-range PCR primers amplified the full-length of HLA class I genes (A, B, C from promotor to 3' UTR. Class II genes (DRB1, DQB1 were amplified from exon 2 through part of exon 4. PCR amplicons were pooled and sheared using Covaris fragmentation. Library preparation was performed using the Illumina TruSeq Nano kit on the Beckman FX automated platform. Each sample was tagged with a unique barcode, followed by 2×250 bp paired-end sequencing on the Illumina MiSeq. HLA typing was assigned using Omixon Twin software that combines two independent computational algorithms to ensure high confidence in allele calling. Consensus sequence and typing results were reported in Histoimmunogenetics Markup Language (HML format. All homozygous alleles were confirmed by Luminex SSO typing and exon novelties were confirmed by Sanger sequencing.Using this automated workflow, over 10,063 NMDP registry donors were successfully typed under high-resolution by NGS. Despite known challenges of nucleic acid degradation and low DNA concentration commonly associated with buccal-based specimens, 97.8% of samples were successfully amplified using long-range PCR. Among these, 98.2% were successfully reported by NGS, with an accuracy rate of 99.84% in an independent blind Quality Control audit performed by the NDMP. In this study, NGS-HLA typing identified 23 null alleles (0.023%, 92 rare alleles (0.091% and 42 exon novelties (0.042%.Long-range
Yin, Yuxin; Lan, James H; Nguyen, David; Valenzuela, Nicole; Takemura, Ping; Bolon, Yung-Tsi; Springer, Brianna; Saito, Katsuyuki; Zheng, Ying; Hague, Tim; Pasztor, Agnes; Horvath, Gyorgy; Rigo, Krisztina; Reed, Elaine F; Zhang, Qiuheng
Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT), HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS) of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA typing of 10,063 National Marrow Donor Program (NMDP) registry donors using long-range PCR by next generation sequencing (NGS) approach on buccal swab DNA. Multiplex long-range PCR primers amplified the full-length of HLA class I genes (A, B, C) from promotor to 3' UTR. Class II genes (DRB1, DQB1) were amplified from exon 2 through part of exon 4. PCR amplicons were pooled and sheared using Covaris fragmentation. Library preparation was performed using the Illumina TruSeq Nano kit on the Beckman FX automated platform. Each sample was tagged with a unique barcode, followed by 2×250 bp paired-end sequencing on the Illumina MiSeq. HLA typing was assigned using Omixon Twin software that combines two independent computational algorithms to ensure high confidence in allele calling. Consensus sequence and typing results were reported in Histoimmunogenetics Markup Language (HML) format. All homozygous alleles were confirmed by Luminex SSO typing and exon novelties were confirmed by Sanger sequencing. Using this automated workflow, over 10,063 NMDP registry donors were successfully typed under high-resolution by NGS. Despite known challenges of nucleic acid degradation and low DNA concentration commonly associated with buccal-based specimens, 97.8% of samples were successfully amplified using long-range PCR. Among these, 98.2% were successfully reported by NGS, with an accuracy rate of 99.84% in an independent blind Quality Control audit performed by the NDMP. In this study, NGS-HLA typing identified 23 null alleles (0.023%), 92 rare alleles (0.091%) and 42 exon novelties (0.042%). Long-range
Koon Poh Bee
Full Text Available Abstract Background Ethnic differences in body fat distribution contribute to ethnic differences in cardiovascular morbidities and diabetes. However few data are available on differences in fat distribution in Asian children from various backgrounds. Therefore, the current study aimed to explore ethnic differences in body fat distribution among Asian children from four countries. Methods A total of 758 children aged 8-10 y from China, Lebanon, Malaysia and Thailand were recruited using a non-random purposive sampling approach to enrol children encompassing a wide BMI range. Height, weight, waist circumference (WC, fat mass (FM, derived from total body water [TBW] estimation using the deuterium dilution technique and skinfold thickness (SFT at biceps, triceps, subscapular, supraspinale and medial calf were collected. Results After controlling for height and weight, Chinese and Thai children had a significantly higher WC than their Lebanese and Malay counterparts. Chinese and Thais tended to have higher trunk fat deposits than Lebanese and Malays reflected in trunk SFT, trunk/upper extremity ratio or supraspinale/upper extremity ratio after adjustment for age and total body fat. The subscapular/supraspinale skinfold ratio was lower in Chinese and Thais compared with Lebanese and Malays after correcting for trunk SFT. Conclusions Asian pre-pubertal children from different origins vary in body fat distribution. These results indicate the importance of population-specific WC cut-off points or other fat distribution indices to identify the population at risk of obesity-related health problems.
Bodine, D. J.; Rasmussen, K. L.
Understanding microphysical processes in nocturnal mesoscale convective systems (MCSs) is an important objective of the Plains Elevated Convection At Night (PECAN) experiment, which occurred from 1 June - 15 July 2015 in the central Great Plains region of the United States. Observations of MCSs were collected using a large array of mobile and fixed instrumentation, including ground-based radars, soundings, PECAN Integrated Sounding Arrays (PISAs), and aircraft. In addition to these observations, three mobile Parsivel disdrometers were deployed to obtain drop-size distribution (DSD) measurements to further explore microphysical processes in convective and stratiform regions of nocturnal MCSs. Disdrometers were deployed within close range of a multiple frequency network of mobile and fixed dual-polarization radars (5 - 30 km range), and near mobile sounding units and PISAs. Using mobile disdrometer and multiple-wavelength, dual-polarization radar data, microphysical properties of convective and stratiform regions of MCSs are investigated. The analysis will also examine coordinated Range-Height Indicator (RHI) scans over the disdrometers to elucidate vertical DSD structure. Analysis of dense observations obtained during PECAN in combination with mobile disdrometer DSD measurements contributes to a greater understanding of the structural characteristics and evolution of nocturnal MCSs.
Full Text Available In order to investigate the aerosol variability over the southwest region of Slovenia, an ultraviolet scanning elastic backscatter LiDAR was utilized to make the vertical scan for atmospheric probing. With the assumption of horizontal atmospheric homogeneity, aerosol optical variables were retrieved from the horizontal pixel data points of two-dimensional range-height-indicator (RHI diagrams by using a multiangle retrieval method, in which optical depth is defined as the slope of the resulting linear function when height is kept constant. To make the data retrieval feasible and precise, a series of key procedures complemented the data processing, including construction of the RHI diagram, correction of Rayleigh scattering, assessment of horizontal atmospheric homogeneity and retrieval of aerosol optical variables. The measurement example demonstrated the feasibility of the ultraviolet scanning elastic backscatter LiDAR in the applications of the retrieval of aerosol extinction and determination of the atmospheric boundary layer height. Three months’ data combined with the modeling of air flow trajectories using Hybrid Single Particle Lagrangian Integrated Trajectory Model were analyzed to investigate aerosol variability. The average value of aerosol extinction with the presence of land-based air masses from the European continent was found to be two-times larger than that influenced by marine aerosols from the Mediterranean or Adriatic Sea.
Mariani, Zen; Dehghan, Armin; Gascon, Gabrielle; Joe, Paul; Hudak, David; Strawbridge, Kevin; Corriveau, Julien
Data collected between October 2015 and May 2016 at Environment and Climate Change Canada's Iqaluit research site (64°N, 69°W) have revealed a high frequency (40% of all days for which observations were available) of stratified wind layer events that occur from near the surface up to about 7.2 km above sea level. These stratified wind layers are clearly visible as wind shifts (90 to 180°) with height in range-height indicator scans from the Doppler lidar and Ka-band radar and in wind direction profiles from the Doppler lidar and radiosonde. During these events, the vertical structure of the flow appears to be a stack of 4 to 10 layers ranging in vertical width from 0.1 to 4.4 km. The stratification events that were observed occurred predominantly (81%) during light precipitation and lasted up to 27.5 h. The integrated measurement platforms at Iqaluit permitted continuous observations of the evolution of stratification events in different meteorological conditions.
Kollias, Pavlos [McGill Univ., Montreal, QC (Canada); Jo, Ieng [McGill Univ., Montreal, QC (Canada); Borque, Paloma [McGill Univ., Montreal, QC (Canada); Tatarevic, Aleksandra [McGill Univ., Montreal, QC (Canada); Lamer, Katia [McGill Univ., Montreal, QC (Canada); Bharadwaj, Nitin [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Widener, Kevin B. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Johnson, Karen [Brookhaven National Lab. (BNL), Upton, NY (United States); Clothiaux, Eugene E. [Pennsylvania State Univ., State College, PA (United States)
The Scanning ARM Cloud Radars (SACR’s) are the primary instruments for documenting the four-dimensional structure and evolution of clouds within a 20-30 km radius from the ARM fixed and mobile sites. Here, the post-processing of the calibrated SACR measurements is discussed. First, a feature mask algorithm that objectively determines the presence of significant radar returns is described. The feature mask algorithm is based on the statistical properties of radar receiver noise. It accounts for atmospheric emission and is applicable even for SACR profiles with few or no signal-free range gates. Using the nearest-in-time atmospheric sounding, the SACR radar reflectivities are corrected for gaseous attenuation (water vapor and oxygen) using a line-by-line absorption model. Despite having a high pulse repetition frequency, the SACR has a narrow Nyquist velocity limit and thus Doppler velocity folding is commonly observed. An unfolding algorithm that makes use of a first guess for the true Doppler velocity using horizontal wind measurements from the nearest sounding is described. The retrieval of the horizontal wind profile from the Hemispherical Sky – Range Height Indicator SACR scan observations and/or nearest sounding is described. The retrieved horizontal wind profile can be used to adaptively configure SACR scan strategies that depend on wind direction. Several remaining challenges are discussed, including the removal of insect and second-trip echoes. The described algorithms significantly enhance SACR data quality and constitute an important step towards the utilization of SACR measurements for cloud research.
Sun Zheng; Ning Hui; Tang Jing; Xie Yong-Jie; Shi Peng-Fei; Wang Jian-Hua; Wang Ke
Atmospheric duct is a common phenomenon over large bodies of water, and it can significantly affect the performance of many radio systems. In this paper, a two-month (in July and August, 2014) sounding experiment in ducting conditions over Bosten Lake was carried out at a littoral station (41.89° N, 87.22° E) with high resolution GPS radiosondes, and atmospheric ducts were observed for the first time in this area. During the two months, surface and surface-based ducts occurred frequently over the Lake. Strong diurnal variations in ducting characteristics were noticed in clear days. Ducting occurrence was found at its lowest in the early morning and at its highest (nearly 100%) in the afternoon. Duct strength was found increasing from early morning to forenoon, and reaching its maximum in the afternoon. But contrarily, duct altitude experienced a decrease in a clear day. Then the meteorological reasons for the variations were discussed in detail, turbulent bursting was a possible reason for the duct formation in the early morning and the prevailing lake-breeze front was the main reason in the afternoon. The propagation of electromagnetic wave in a ducting environment was also investigated. A ray-tracing framework based on Runge–Kutta method was proposed to assess the performance of radio systems, and the precise critical angle and grazing angle derived from the ray-tracing equations were provided. Finally, numerical investigations on the radar performance in the observed ducting environments have been carried out with high accuracy, which demonstrated that atmospheric ducts had made great impacts on the performance of radio systems. The range/height errors for radar measurement induced by refraction have also been presented, too, which shows that the height errors were very large for trapped rays when the total range was long enough. (paper)
Full Text Available We present a 3-D climatology of the desert dust distribution over South and East Asia derived using CALIPSO (Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation data. To distinguish desert dust from total aerosol load we apply a methodology developed in the framework of EARLINET (European Aerosol Research Lidar Network. The method involves the use of the particle linear depolarization ratio and updated lidar ratio values suitable for Asian dust, applied to multiyear CALIPSO observations (January 2007–December 2015. The resulting dust product provides information on the horizontal and vertical distribution of dust aerosols over South and East Asia along with the seasonal transition of dust transport pathways. Persistent high D_AOD (dust aerosol optical depth values at 532 nm, of the order of 0.6, are present over the arid and semi-arid desert regions. Dust aerosol transport (range, height and intensity is subject to high seasonality, with the highest values observed during spring for northern China (Taklimakan and Gobi deserts and during summer over the Indian subcontinent (Thar Desert. Additionally, we decompose the CALIPSO AOD (aerosol optical depth into dust and non-dust aerosol components to reveal the non-dust AOD over the highly industrialized and densely populated regions of South and East Asia, where the non-dust aerosols yield AOD values of the order of 0.5. Furthermore, the CALIPSO-based short-term AOD and D_AOD time series and trends between January 2007 and December 2015 are calculated over South and East Asia and over selected subregions. Positive trends are observed over northwest and east China and the Indian subcontinent, whereas over southeast China trends are mostly negative. The calculated AOD trends agree well with the trends derived from Aqua MODIS (Moderate Resolution Imaging Spectroradiometer, although significant differences are observed over specific regions.
Prasicek, Günther; Robl, Jörg; Lang, Andreas
Glacial cirques are morphologically distinct but complex landforms and represent a vital part of high mountain topography. Their distribution, elevation and relief are expected to hold information on (1) the extent of glacial occupation, (2) the mechanism of glacial cirque erosion, and (3) how glacial in concert with periglacial processes can limit peak altitude and mountain range height. While easily detectably for the expert's eye both in nature and on various representations of topography, their complicated nature makes them a nemesis for computer algorithms. Consequently, manual mapping of glacial cirques is commonplace in many mountain landscapes worldwide, but consistent datasets of cirque distribution and objectively mapped cirques and their morphometrical attributes are lacking. Among the biggest problems for algorithm development are the complexity in shape and the great variability of cirque size. For example, glacial cirques can be rather circular or longitudinal in extent, exist as individual and composite landforms, show prominent topographic depressions or can entirely be filled with water or sediment. For these reasons, attributes like circularity, size, drainage area and topology of landform elements (e.g. a flat floor surrounded by steep walls) have only a limited potential for automated cirque detection. Here we present a novel, geomorphometric method for automated identification of glacial cirques on digital elevation models that exploits their genetic bowl-like shape. First, we differentiate between glacial and fluvial terrain employing an algorithm based on a moving window approach and multi-scale curvature, which is also capable of fitting the analysis window to valley width. We then fit a plane to the valley stretch clipped by the analysis window and rotate the terrain around the center cell until the plane is level. Doing so, we produce sinks of considerable size if the clipped terrain represents a cirque, while no or only very small sinks
Gomes, F K; Oliveira, M D B L; Homem, B G C; Boddey, R M; Bernardes, T F; Gionbelli, M P; Lara, M A S; Casagrande, D R
of microbial synthesis (P = 0.023) were greater at 95LI and 90LI, followed by 42D and less at 100LI. Overall, the range from 90 to 95% of LI is the recommendation to interrupt the rest period, since this strategy enhanced community stability, forage intake, and nutritional value of the diet. Under on-farm conditions, brachiaria grass and forage peanut pastures should be managed at a range height of 24 to 30 cm.
Wulfmeyer, Volker [University of Hohenheim; Turner, David [NOAA National Severe Storms Laboratory
The Land-Atmosphere Feedback Experiment (LAFE; pronounced “la-fey”) deploys several state-of-the-art scanning lidar and remote sensing systems to the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility’s Southern Great Plains (SGP) site. These instruments will augment the ARM instrument suite in order to collect a data set for studying feedback processes between the land surface and the atmosphere. The novel synergy of remote-sensing systems will be applied for simultaneous measurements of land-surface fluxes and horizontal and vertical transport processes in the atmospheric convective boundary layer (CBL). The impact of spatial inhomogeneities of the soil-vegetation continuum on land-surface-atmosphere (LSA) feedback will be studied using the scanning capability of the instrumentation. The time period of the observations is August 2017, because large differences in surface fluxes between different fields and bare soil can be observed, e.g., pastures versus fields where the wheat has already been harvested. The remote sensing system synergy will consist of three components: 1) The SGP water vapor and temperature Raman lidar (SRL), the SGP Doppler lidar (SDL), and the National Center for Atmospheric Research (NCAR) water vapor differential absorption lidar (DIAL) (NDIAL) mainly in vertical staring modes to measure mean profiles and gradients of moisture, temperature, and horizontal wind. They will also measure profiles of higher-order turbulent moments in the water vapor and wind fields and profiles of the latent heat flux. 2) A novel scanning lidar system synergy consisting of the National Oceanic and Atmospheric Administration (NOAA) High-Resolution Doppler lidar (HRDL), the University of Hohenheim (UHOH) water-vapor differential absorption lidar (UDIAL), and the UHOH temperature Raman lidar (URL). These systems will perform coordinated range-height indicator (RHI) scans from just above the canopy level to the
Ramos-Izquierdo, Luis A.
A diffractive optic element (DOE) can be used as a beam splitter to generate multiple laser beams from a single input laser beam. This technology has been recently used in LRO s Lunar Orbiter Laser Altimeter (LOLA) instrument to generate five laser beams that measure the lunar topography from a 50-km nominal mapping orbit (see figure). An extension of this approach is to use a multiple-zone DOE to allow a laser altimeter instrument to operate over a wider range of distances. In particular, a multiple-zone DOE could be used for applications that require both mapping and landing on a planetary body. In this case, the laser altimeter operating range would need to extend from several hundred kilometers down to a few meters. The innovator was recently involved in an investigation how to modify the LOLA instrument for the OSIRIS asteroid mapping and sample return mission. One approach is to replace the DOE in the LOLA laser beam expander assembly with a multiple-zone DOE that would allow for the simultaneous illumination of the asteroid with mapping and landing laser beams. The proposed OSIRIS multiple-zone DOE would generate the same LOLA five-beam output pattern for high-altitude topographic mapping, but would simultaneously generate a wide divergence angle beam using a small portion of the total laser energy for the approach and landing portion of the mission. Only a few percent of the total laser energy is required for approach and landing operations as the return signal increases as the inverse square of the ranging height. A wide divergence beam could be implemented by making the center of the DOE a diffractive or refractive negative lens. The beam energy and beam divergence characteristics of a multiple-zone DOE could be easily tailored to meet the requirements of other missions that require laser ranging data. Current single-zone DOE lithographic manufacturing techniques could also be used to fabricate a multiple-zone DOE by masking the different DOE zones during