Sample records for rps structure analysis

  1. Fault tree analysis of KNICS RPS software

    Park, Gee Yong; Kwon, Kee Choon [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Koh, Kwang Yong; Jee, Eun Kyoung; Seong, Poong Hyun [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of); Lee, Dae Hyung [Doosan Heavy Industries and Construction, Yongin (Korea, Republic of)


    This paper describes the application of a software Fault Tree Analysis (FTA) as one of the analysis techniques for a Software Safety Analysis (SSA) at the design phase and its analysis results for the safety-critical software of a digital reactor protection system, which is called the KNICS RPS, being developed in the KNICS (Korea Nuclear Instrumentation and Control Systems) project. The software modules in the design description were represented by Function Blocks (FBs), and the software FTA was performed based on the well-defined fault tree templates for the FBs. The SSA, which is part of the verification and validation (V and V) activities, was activated at each phase of the software lifecycle for the KNICS RPS. At the design phase, the software HAZOP (Hazard and Operability) and the software FTA were employed in the SSA in such a way that the software HAZOP was performed first and then the software FTA was applied. The software FTA was applied to some critical modules selected from the software HAZOP analysis.

  2. Yeast strains with N-terminally truncated ribosomal protein S5: implications for the evolution, structure and function of the Rps5/Rps7 proteins.

    Lumsden, Thomas; Bentley, Amber A; Beutler, William; Ghosh, Arnab; Galkin, Oleksandr; Komar, Anton A


    Ribosomal protein (rp)S5 belongs to the family of the highly conserved rp's that contains rpS7 from prokaryotes and rpS5 from eukaryotes. Alignment of rpS5/rpS7 from metazoans (Homo sapiens), fungi (Saccharomyces cerevisiae) and bacteria (Escherichia coli) shows that the proteins contain a conserved central/C-terminal core region and possess variable N-terminal regions. Yeast rpS5 is 69 amino acids (aa) longer than the E. coli rpS7 protein; and human rpS5 is 48 aa longer than the rpS7, respectively. To investigate the function of the yeast rpS5 and in particular the role of its N-terminal region, we obtained and characterized yeast strains in which the wild-type yeast rpS5 was replaced by its truncated variants, lacking 13, 24, 30 and 46 N-terminal amino acids, respectively. All mutant yeast strains were viable and displayed only moderately reduced growth rates, with the exception of the strain lacking 46 N-terminal amino acids, which had a doubling time of about 3 h. Biochemical analysis of the mutant yeast strains suggests that the N-terminal part of the eukaryotic and, in particular, yeast rpS5 may impact the ability of 40S subunits to function properly in translation and affect the efficiency of initiation, specifically the recruitment of initiation factors eIF3 and eIF2.

  3. Validation Database Based Thermal Analysis of an Advanced RPS Concept

    Balint, Tibor S.; Emis, Nickolas D.


    Advanced RPS concepts can be conceived, designed and assessed using high-end computational analysis tools. These predictions may provide an initial insight into the potential performance of these models, but verification and validation are necessary and required steps to gain confidence in the numerical analysis results. This paper discusses the findings from a numerical validation exercise for a small advanced RPS concept, based on a thermal analysis methodology developed at JPL and on a validation database obtained from experiments performed at Oregon State University. Both the numerical and experimental configurations utilized a single GPHS module enabled design, resembling a Mod-RTG concept. The analysis focused on operating and environmental conditions during the storage phase only. This validation exercise helped to refine key thermal analysis and modeling parameters, such as heat transfer coefficients, and conductivity and radiation heat transfer values. Improved understanding of the Mod-RTG concept through validation of the thermal model allows for future improvements to this power system concept.

  4. (RPS28) from the Giant Panda



    Jun 3, 2009 ... Primary structure analysis revealed that the molecular weight of the putative RPS28 protein is ... a variety of mechanisms including transcriptional and ... amplify the cDNA of RPS28 gene from the total RNA and .... amplified RPS28. Figure 2. Polymerase Chain Reaction Products ... On the basis of the high.

  5. Conservation of the structure and organization of lupin mitochondrial nad3 and rps12 genes.

    Rurek, M; Oczkowski, M; Augustyniak, H


    A high level of the nucleotide sequence conservation of mitochondrial nad3 and rps12 genes was found in four lupin species. The only differences concern three nucleotides in the Lupinus albus rps12 gene and three nucleotides insertion in the L. mutabilis spacer. Northern blot analysis as well as RT-PCR confirmed cotranscription of the L. luteus genes because the transcripts detected were long enough.

  6. Dexterity Analysis of a 4-UPS-RPS Parallel Mechanism

    Xiulong Chen


    Full Text Available A novel 4‐UPS‐RPS spatial 5 degrees of freedom parallel mechanism is introduced and its dexterity measures are analysed. The stationary platform of the parallel mechanism is connected with the moving platform by the same four UPS (universal joints‐prismatic pairs‐spherical joints driving links and a RPS (revolute joints‐prismatic pairs‐spherical joints link. The velocity Jacobian matrix and the force Jacobian matrix of the parallel mechanism are derived, and the dimensionless velocity Jacobian matrix is established. On this basis, the three dexterity measures, including average conditional number, the smallest singular value and operation, are obtained. According to these, the square mean dexterity coefficient, which is used to comprehensively evaluate the dexterity of various configurations, is proposed. In addition, the distribution of different dexterity measures above in the workspace is analysed respectively. This research can provide an important theoretical base for the dimensional synthesis and trajectory planning for a parallel mechanism.

  7. Targeted resequencing and analysis of the Diamond-Blackfan anemia disease locus RPS19.

    Alvaro Martinez Barrio

    Full Text Available BACKGROUND: The Ribosomal protein S19 gene locus (RPS19 has been linked to two kinds of red cell aplasia, Diamond-Blackfan Anemia (DBA and Transient Erythroblastopenia in Childhood (TEC. Mutations in RPS19 coding sequences have been found in 25% of DBA patients, but not in TEC patients. It has been suggested that non-coding RPS19 sequence variants contribute to the considerable clinical variability in red cell aplasia. We therefore aimed at identifying non-coding variations associated with DBA or TEC phenotypes. METHODOLOGY/PRINCIPAL FINDINGS: We targeted a region of 19'980 bp encompassing the RPS19 gene in a cohort of 89 DBA and TEC patients for resequencing. We provide here a catalog of the considerable, previously unrecognized degree of variation in this region. We identified 73 variations (65 SNPs, 8 indels that all are located outside of the RPS19 open reading frame, and of which 67.1% are classified as novel. We hypothesize that specific alleles in non-coding regions of RPS19 could alter the binding of regulatory proteins or transcription factors. Therefore, we carried out an extensive analysis to identify transcription factor binding sites (TFBS. A series of putative interaction sites coincide with detected variants. Sixteen of the corresponding transcription factors are of particular interest, as they are housekeeping genes or show a direct link to hematopoiesis, tumorigenesis or leukemia (e.g. GATA-1/2, PU.1, MZF-1. CONCLUSIONS: Specific alleles at predicted TFBSs may alter the expression of RPS19, modify an important interaction between transcription factors with overlapping TFBS or remove an important stimulus for hematopoiesis. We suggest that the detected interactions are of importance for hematopoiesis and could provide new insights into individual response to treatment.

  8. Architecture dependent availability analysis of RPS for Research Reactor Applications

    Rahman, Khalilur; Heo, Gyunyoung [Kyung Hee Univ., Yongin (Korea, Republic of); Son, Hanseong [Joongbu Univ., Geumsan (Korea, Republic of); Kim, Youngki; Park, Jaekwan [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)


    The research reactors are categorized into two broad categories, Low power research reactors and medium to high power research reactors. According to IAEA TECDOC-1234, Research reactors with 0.250- 2.0 MW power rating or 2.5-10 Χ 10{sup 11} η/cm{sup 2}. s flux are termed low power reactor whereas research reactors ranging from 2-10 MW power rating or 0.1-10 Χ 10{sup 13} η/cm{sup 2}. s are considered as Medium to High power research reactors. Some other standards (IAEA NP-T-5.1) define multipurpose research reactor ranging from power few hundred KW to 10 MW as low power research reactor. The aim of this research, in this article, was to identify a configuration of architecture which gives highest availability with maintaining low cost of manufacturing. In this regard, two configurations of a single channel of RPS are formulated in the current article and their fault trees were developed using AIMS PSA software to get the unavailability. This is a starting point of attempt towards the standardization of I and C architecture for low and medium power research reactors.

  9. Compliance modeling and analysis of a 3-RPS parallel kinematic machine module

    Zhang, Jun; Zhao, Yanqin; Dai, Jiansheng


    The compliance modeling and rigidity performance evaluation for the lower mobility parallel manipulators are still to be remained as two overwhelming challenges in the stage of conceptual design due to their geometric complexities. By using the screw theory, this paper explores the compliance modeling and eigencompliance evaluation of a newly patented 1T2R spindle head whose topological architecture is a 3-RPS parallel mechanism. The kinematic definitions and inverse position analysis are briefly addressed in the first place to provide necessary information for compliance modeling. By considering the 3-RPS parallel kinematic machine(PKM) as a typical compliant parallel device, whose three limb assemblages have bending, extending and torsional deflections, an analytical compliance model for the spindle head is established with screw theory and the analytical stiffness matrix of the platform is formulated. Based on the eigenscrew decomposition, the eigencompliance and corresponding eigenscrews are analyzed and the platform's compliance properties are physically interpreted as the suspension of six screw springs. The distributions of stiffness constants of the six screw springs throughout the workspace are predicted in a quick manner with a piece-by-piece calculation algorithm. The numerical simulation reveals a strong dependency of platform's compliance on its configuration in that they are axially symmetric due to structural features. At the last stage, the effects of some design variables such as structural, configurational and dimensional parameters on system rigidity characteristics are investigated with the purpose of providing useful information for the structural design and performance improvement of the PKM. Compared with previous efforts in compliance analysis of PKMs, the present methodology is more intuitive and universal thus can be easily applied to evaluate the overall rigidity performance of other PKMs with high efficiency.

  10. The human RPS4 paralogue on Yq11.223 encodes a structurally conserved ribosomal protein and is preferentially expressed during spermatogenesis

    Ellis Peter J


    Full Text Available Abstract Background The Y chromosome of mammals is particularly prone to accumulate genes related to male fertility. However, the high rate of molecular evolution on this chromosome predicts reduced power to the across-species comparative approach in identifying male-specific genes that are essential for sperm production in humans. We performed a comprehensive analysis of expression of Y-linked transcripts and their X homologues in several human tissues, and in biopsies of infertile patients, in an attempt to identify new testis-specific genes involved in human spermatogenesis. Results We present evidence that one of the primate-specific Y-linked ribosomal protein genes, RPS4Y2, has restricted expression in testis and prostate, in contrast with its X-linked homologue, which is ubiquitously expressed. Moreover, we have determined by highly specific quantitative real time PCR that RPS4Y2 is more highly expressed in testis biopsies containing germ cells. The in silico analysis of the promoter region of RPS4Y2 revealed several differences relative to RPS4Y1, the more widely expressed paralogue from which Y2 has originated through duplication. Finally, through comparative modelling we obtained the three dimensional models of the human S4 proteins, revealing a conserved structure. Interestingly, RPS4Y2 shows different inter-domain contacts and the potential to establish specific interactions. Conclusions These results suggest that one of the Y-linked copies of the ribosomal protein S4 is preferentially expressed during spermatogenesis and might be important for germ cell development. Even though RPS4Y2 has accumulated several amino acid changes following its duplication from RPS4Y1, approximately 35 million years ago, the evolution of the Y-encoded RPS4 proteins is structurally constrained. However, the exclusive expression pattern of RPS4Y2 and the novelties acquired at the C-terminus of the protein may indicate some degree of functional

  11. Singularity Analysis of a 3-RPS Parallel Manipulator Using Geometric Algebra

    LI Qinchuan; XIANG Ji’nan; CHAI Xinxue; WU Chuanyu


    Singular configurations must be avoided in path planning and control of a parallel manipulator. However, most studies rarely focus on an overall singularity loci distribution of lower-mobility parallel mechanisms. Geometric algebra is employed in analysis of singularity of a 3-RPS parallel manipulator. Twist and wrench in screw theory are represented in geometric algebra. Linear dependency of twists and wrenches are described by outer product in geometric algebra. Reciprocity between twists and constraint wrenches are reflected by duality. To compute the positions of the three spherical joints of the 3-RPS parallel manipulator, Tilt-and-Torsion angles are used to describe the orientation of the moving platform. The outer product of twists and constraint wrenches is used as an index for closeness to singularity(ICS) of the 3-RPS parallel manipulator. An overall and thorough perspective of the singularity loci distribution of the 3-RPS parallel manipulator is disclosed, which is helpful to design, trajectory planning and control of this kind of parallel manipulator.

  12. Singularity analysis of a 3-RPS parallel manipulator using geometric algebra

    Li, Qinchuan; Xiang, Ji'nan; Chai, Xinxue; Wu, Chuanyu


    Singular configurations must be avoided in path planning and control of a parallel manipulator. However, most studies rarely focus on an overall singularity loci distribution of lower-mobility parallel mechanisms. Geometric algebra is employed in analysis of singularity of a 3-RPS parallel manipulator. Twist and wrench in screw theory are represented in geometric algebra. Linear dependency of twists and wrenches are described by outer product in geometric algebra. Reciprocity between twists and constraint wrenches are reflected by duality. To compute the positions of the three spherical joints of the 3-RPS parallel manipulator, Tilt-and-Torsion angles are used to describe the orientation of the moving platform. The outer product of twists and constraint wrenches is used as an index for closeness to singularity(ICS) of the 3-RPS parallel manipulator. An overall and thorough perspective of the singularity loci distribution of the 3-RPS parallel manipulator is disclosed, which is helpful to design, trajectory planning and control of this kind of parallel manipulator.

  13. Geneticand Protein Sequence Analysis of rpS29 from Ailuropoda melanoleuca (giant panda)

    Jie Deng; Xiang Ding; Wanru Hou; Jie Zhong; Li Luo; Bo Song; Ting Wang; Yiling Hou


      ToexplorethestructuralcharacteristicofribosomalproteinS29(rpS29)geneofAiluropodamelanoleuca (giantpanda),primersweredesignedbasedontheknownnucleotidesequenceofrpS29genestoclonethecDNAand genomicsequencesofthisgenefromgiantpandabyRT-PCRandPCRstrategyrespectively,andthentheclonedcDNA andgenomicsequencesweresequencedandanalyzedpreliminarily.TheresultsindicatedthatthecDNAfragmentofthe rpS29fromthegiantpandais205bpinsizeinlength,containinganopenreadingframe(ORF)of171bp,encoding56 aminoacids.Thelengthofthegenomicsequenceis1598bp,withthreeexonsandtwointrons.Thecodingsequence showsahighdegreeofhomologytothoseof Homosapiens,Bostaurus,Rattusnorvegicusand Musmusculus with 75%,94.74%,89.47%and88.89%respectively,Whileallthehomologiesforaminoacidsequencesarehighupto 100%.PrimarystructureanalysisrevealedthatthemolecularweightoftheputativerpS29proteinis6.68KD,witha theoreticalpIof10.63.Basedontopologyprediction,therearethreedistincttypesoffunctionalsitesintherpS29 proteinofgiantpanda.

  14. A novel additional group II intron distinguishes the mitochondrial rps3 gene in gymnosperms.

    Regina, Teresa M R; Picardi, Ernesto; Lopez, Loredana; Pesole, Graziano; Quagliariello, Carla


    Comparative analysis of the ribosomal protein S3 gene (rps3) in the mitochondrial genome of Cycas with newly sequenced counterparts from Magnolia and Helianthus and available sequences from higher plants revealed that the positional clustering with the genes for ribosomal protein S19 (rps19) and L16 (rpl16) is preserved in gymnosperms. However, in contrast to the other land plant species, the rps3 gene in Cycas mitochondria is unique in possessing a second intron: rps3i2. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the transcripts generated from the rps19-rps3-rpl16 cluster in Cycas mitochondria demonstrated that the genes are cotranscribed and extensively modified by RNA editing and that both introns are efficiently spliced. Despite remarkable size heterogeneity, the Cycas rps3i1 can be shown to be homologous to the group IIA introns present within the rps3 gene of algae and land plants, including Magnolia and Helianthus. Conversely, sequences similar to the rps3i2 have not been reported previously. On the basis of conserved primary and secondary structure the second intervening sequence interrupting the Cycas rps3 gene has been classified as a group II intron. The close relationship of the rps3i2 to a group of different plant mitochondrial introns is intriguing and suggestive of a mitochondrial derivation for this novel intervening sequence. Interestingly, the rps3i2 appears to be conserved at the same gene location in other gymnosperms. Furthermore, the pattern of the rps3i2 distribution among algae and land plants provides evidence for the evolutionary acquisition of this novel intron in gymnosperms via intragenomic transposition or retrotransposition.

  15. cDNA, genomic sequence cloning and overexpression of ribosomal protein S25 gene (RPS25) from the Giant Panda.

    Hao, Yan-Zhe; Hou, Wan-Ru; Hou, Yi-Ling; Du, Yu-Jie; Zhang, Tian; Peng, Zheng-Song


    RPS25 is a component of the 40S small ribosomal subunit encoded by RPS25 gene, which is specific to eukaryotes. Studies in reference to RPS25 gene from animals were handful. The Giant Panda (Ailuropoda melanoleuca), known as a "living fossil", are increasingly concerned by the world community. Studies on RPS25 of the Giant Panda could provide scientific data for inquiring into the hereditary traits of the gene and formulating the protective strategy for the Giant Panda. The cDNA of the RPS25 cloned from Giant Panda is 436 bp in size, containing an open reading frame of 378 bp encoding 125 amino acids. The length of the genomic sequence is 1,992 bp, which was found to possess four exons and three introns. Alignment analysis indicated that the nucleotide sequence of the coding sequence shows a high homology to those of Homo sapiens, Bos taurus, Mus musculus and Rattus norvegicus as determined by Blast analysis, 92.6, 94.4, 89.2 and 91.5%, respectively. Primary structure analysis revealed that the molecular weight of the putative RPS25 protein is 13.7421 kDa with a theoretical pI 10.12. Topology prediction showed there is one N-glycosylation site, one cAMP and cGMP-dependent protein kinase phosphorylation site, two Protein kinase C phosphorylation sites and one Tyrosine kinase phosphorylation site in the RPS25 protein of the Giant Panda. The RPS25 gene was overexpressed in E. coli BL21 and Western Blotting of the RPS25 protein was also done. The results indicated that the RPS25 gene can be really expressed in E. coli and the RPS25 protein fusioned with the N-terminally his-tagged form gave rise to the accumulation of an expected 17.4 kDa polypeptide. The cDNA and the genomic sequence of RPS25 were cloned successfully for the first time from the Giant Panda using RT-PCR technology and Touchdown-PCR, respectively, which were both sequenced and analyzed preliminarily; then the cDNA of the RPS25 gene was overexpressed in E. coli BL21 and immunoblotted, which is the first

  16. Testing and distribution of the RPS2 proposal submission system

    Douglas, Robert E.


    In 1995, the Space Telescope Science Institute (STScI) introduced RPS2 (Remote Proposal Submission 2). RPS2 is used by Hubble Space TElescope (HST) proposers to prepare their detailed observation descriptions. It is a client/server system implemented using Tcl/Tk. The client can transparently access servers on the user's machine, at STScI, or on any other machine on the Internet. The servers combine syntax checking, feasibility analysis and orbit packing, and constraint and schedulability analysis of user- specified proposals as they will be performed aboard HST. Prior to the release of RPS2, observers used a system which provided only syntax checking. RPS2 now provides the observers with some of the more complicated pieces of software that had been used by STScI staff to prepare observations since 1990. The RPS2 system consists of four independent subsystem, controlled by the client/server mechanism. A problem with a system of this size and complexity is that the software components, which continue to grow and change with HST itself, must continually be tested and distributed to those who need it. In the past, it had been acceptable to release the RPS2 software only once per observing cycle, but it became apparent before the 1997 HST Servicing Mission that multiple releases of RPS2 were going to be required to support the new instruments. This paper discusses how RPS2 and its component systems are maintained, updated, tested, and distributed.

  17. NASA RPS Program Overview: A Focus on RPS Users

    Hamley, John A.; Sutliff, Thomas J.; Sandifer, Carl E., II; Zakrajsek, June F.


    The goal of NASA's Radioisotope Power Systems (RPS) Program is to make RPS ready and available to support the exploration of the solar system in environments where the use of conventional solar or chemical power generation is impractical or impossible to meet the needs of the missions. To meet this goal, the RPS Program, working closely with the Department of Energy, performs mission and system studies (such as the recently released Nuclear Power Assessment Study), assesses the readiness of promising technologies to infuse in future generators, assesses the sustainment of key RPS capabilities and knowledge, forecasts and tracks the Programs budgetary needs, and disseminates current information about RPS to the community of potential users. This process has been refined and used to determine the current content of the RPS Programs portfolio. This portfolio currently includes an effort to mature advanced thermoelectric technology for possible integration into an enhanced Multi-Mission Radioisotope Generator (eMMRTG), sustainment and production of the currently deployed MMRTG, and technology investments that could lead to a future Stirling Radioisotope Generator (SRG). This paper describes the program planning processes that have been used, the currently available MMRTG, and one of the potential future systems, the eMMRTG.

  18. Transcriptome analysis of the zebrafish model of Diamond-Blackfan anemia from RPS19 deficiency via p53-dependent and -independent pathways.

    Qiong Jia

    Full Text Available Diamond-Blackfan anemia (DBA is a rare inherited bone marrow failure syndrome that is characterized by pure red-cell aplasia and associated physical deformities. It has been proven that defects of ribosomal proteins can lead to this disease and that RPS19 is the most frequently mutated gene in DBA patients. Previous studies suggest that p53-dependent genes and pathways play important roles in RPS19-deficient embryos. However, whether there are other vital factors linked to DBA has not been fully clarified. In this study, we compared the whole genome RNA-Seq data of zebrafish embryos injected with RPS19 morpholino (RPS19 MO, RPS19 and p53 morpholino simultaneously (RPS19+p53 MO and control morpholino (control. We found that genes enriched in the functions of hematological systems, nervous system development and skeletal and muscular disorders had significant differential expression in RPS19 MO embryos compared with controls. Co-inhibition of p53 partially alleviates the abnormalities for RPS19-deficient embryos. However, the hematopoietic genes, which were down-regulated significantly in RPS19 MO embryos, were not completely recovered by the co-inhibition of p53. Furthermore, we identified the genome-wide p53-dependent and -independent genes and pathways. These results indicate that not only p53 family members but also other factors have important impacts on RPS19-deficient embryos. The detection of potential pathogenic genes and pathways provides us a new paradigm for future research on DBA, which is a systematic and complex hereditary disease.

  19. Ribosomal Proteins RPS11 and RPS20, Two Stress-Response Markers of Glioblastoma Stem Cells, Are Novel Predictors of Poor Prognosis in Glioblastoma Patients.

    William H Yong

    Full Text Available Glioblastoma stem cells (GSC co-exhibiting a tumor-initiating capacity and a radio-chemoresistant phenotype, are a compelling cell model for explaining tumor recurrence. We have previously characterized patient-derived, treatment-resistant GSC clones (TRGC that survived radiochemotherapy. Compared to glucose-dependent, treatment-sensitive GSC clones (TSGC, TRGC exhibited reduced glucose dependence that favor the fatty acid oxidation pathway as their energy source. Using comparative genome-wide transcriptome analysis, a series of defense signatures associated with TRGC survival were identified and verified by siRNA-based gene knockdown experiments that led to loss of cell integrity. In this study, we investigate the prognostic value of defense signatures in glioblastoma (GBM patients using gene expression analysis with Probeset Analyzer (131 GBM and The Cancer Genome Atlas (TCGA data, and protein expression with a tissue microarray (50 GBM, yielding the first TRGC-derived prognostic biomarkers for GBM patients. Ribosomal protein S11 (RPS11, RPS20, individually and together, consistently predicted poor survival of newly diagnosed primary GBM tumors when overexpressed at the RNA or protein level [RPS11: Hazard Ratio (HR = 11.5, p<0.001; RPS20: HR = 4.5, p = 0.03; RPS11+RPS20: HR = 17.99, p = 0.001]. The prognostic significance of RPS11 and RPS20 was further supported by whole tissue section RPS11 immunostaining (27 GBM; HR = 4.05, p = 0.01 and TCGA gene expression data (578 primary GBM; RPS11: HR = 1.19, p = 0.06; RPS20: HR = 1.25, p = 0.02; RPS11+RPS20: HR = 1.43, p = 0.01. Moreover, tumors that exhibited unmethylated O-6-methylguanine-DNA methyltransferase (MGMT or wild-type isocitrate dehydrogenase 1 (IDH1 were associated with higher RPS11 expression levels [corr (IDH1, RPS11 = 0.64, p = 0.03; [corr (MGMT, RPS11 = 0.52, p = 0.04]. These data indicate that increased expression of RPS11 and RPS20 predicts shorter patient survival. The study also

  20. 2-RP S & P SR并联机构刚度分析%Stiffness Analysis of 2-RPS & PSR Parallel Mechanism

    柴保明; 韩红雨; 陈景礼; 张保民


    The parallel mechanism is an important development direction of advanced manufacturing, and 2⁃RPS&PSR parallel mechanism is non symmetric mechanism. The Jacobian matrix of the helix theory was used as basis to solve the mechanism, the stiffness of mechanism was obtained. Stiffness analysis was carried out and simulated with Matlab software, and than its various intuitive curve were obtained. It verifies the feasibility of the parallel robot to realize the expected movement, which provides a meaningful reference for the design of this kind of robot, checking and debugging.%并联机构是先进制造领域的一个重要发展方向,2⁃RPS&PSR并联机构是非对称的机构。采用螺旋理论为基础求解该机构的全雅克比矩阵,得出机构的全刚度。进行刚度分析再运用Matlab软件进行仿真,得出其各种直观曲线。验证了该并联机器人实现预期运动的可行性,为该种机器人的设计、校核和调试提供了参考。

  1. Accuracy modulating mutations of the ribosomal protein S4-S5 interface do not necessarily destabilize the rps4-rps5 protein-protein interaction.

    Vallabhaneni, Haritha; Farabaugh, Philip J


    During the process of translation, an aminoacyl tRNA is selected in the A site of the decoding center of the small subunit based on the correct codon-anticodon base pairing. Though selection is usually accurate, mutations in the ribosomal RNA and proteins and the presence of some antibiotics like streptomycin alter translational accuracy. Recent crystallographic structures of the ribosome suggest that cognate tRNAs induce a "closed conformation" of the small subunit that stabilizes the codon-anticodon interactions at the A site. During formation of the closed conformation, the protein interface between rpS4 and rpS5 is broken while new contacts form with rpS12. Mutations in rpS12 confer streptomycin resistance or dependence and show a hyperaccurate phenotype. Mutations reversing streptomycin dependence affect rpS4 and rpS5. The canonical rpS4 and rpS5 streptomycin independent mutations increase translational errors and were called ribosomal ambiguity mutations (ram). The mutations in these proteins are proposed to affect formation of the closed complex by breaking the rpS4-rpS5 interface, which reduces the cost of domain closure and thus increases translational errors. We used a yeast two-hybrid system to study the interactions between the small subunit ribosomal proteins rpS4 and rpS5 and to test the effect of ram mutations on the stability of the interface. We found no correlation between ram phenotype and disruption of the interface.

  2. An 11bp region with stem formation potential is essential for de novo DNA methylation of the RPS element.

    Matthew Gentry

    Full Text Available The initiation of DNA methylation in Arabidopsis is controlled by the RNA-directed DNA methylation (RdDM pathway that uses 24nt siRNAs to recruit de novo methyltransferase DRM2 to the target site. We previously described the REPETITIVE PETUNIA SEQUENCE (RPS fragment that acts as a hot spot for de novo methylation, for which it requires the cooperative activity of all three methyltransferases MET1, CMT3 and DRM2, but not the RdDM pathway. RPS contains two identical 11nt elements in inverted orientation, interrupted by a 18nt spacer, which resembles the features of a stemloop structure. The analysis of deletion/substitution derivatives of this region showed that deletion of one 11nt element RPS is sufficient to eliminate de novo methylation of RPS. In addition, deletion of a 10nt region directly adjacent to one of the 11nt elements, significantly reduced de novo methylation. When both 11nt regions were replaced by two 11nt elements with altered DNA sequence but unchanged inverted repeat homology, DNA methylation was not affected, indicating that de novo methylation was not targeted to a specific DNA sequence element. These data suggest that de novo DNA methylation is attracted by a secondary structure to which the two 11nt elements contribute, and that the adjacent 10nt region influences the stability of this structure. This resembles the recognition of structural features by DNA methyltransferases in animals and suggests that similar mechanisms exist in plants.

  3. Kinematic analysis of 3-RPS type parallel robot based on screw theory%基于螺旋理论的3-RPS型并联机器人运动学分析

    朱大昌; 张国新


    3-RPS type parallel mechanism is of three limbs symmetrical in structure in which each is connected with the base through a revolute pair and a spherical pair is connected with moving platform,While the revolute pair is connected with the spherical pair through a moving pair. Screw theory and spatial mechanism structure theory are adopted to derive the characteristic of motion of the robot through constraints. By applying vector analysis method the normallinverse solution of this mechanism can be derived respectively to obtain a kinematical equation for the mechanism. Furthermore ,the kinematic singularities for the structure of the robot are analyzed based on Jacobine matrix of the mechanism symmetrical with inhomogeneity and imperfect DOF.%3-RPS型并联机构具有三个结构对称的支链形式,各支链由一个转动副连接机座、一个球面副与动平台相连接,转动副与球面副由移动副所连接.采用螺旋理论及空间机构构型原理,通过约束形式分析得出该类型并联机器人运动性质,采用矢量分析方法对其运动学正解/反解进行求解,得出该并联机构运动学方程.基于对称非齐次性少自由度并联机构Jacobine矩阵,进一步对该类型并联机器人结构奇异性进行分析与总结.

  4. Analyses of natural variation indicates that the absence of RPS4/RRS1 and amino acid change in RPS4 cause loss of their functions and resistance to pathogens.

    Narusaka, Mari; Iuchi, Satoshi; Narusaka, Yoshihiro


    A pair of Arabidopsis thaliana resistance proteins, RPS4 and RRS1, recognizes the cognate Avr effector from the bacterial pathogens Pseudomonas syringae pv. tomato expressing avrRps4 (Pst-avrRps4), Ralstonia solanacearum, and the fungal pathogen Colletotrichum higginsianum and leads to defense signaling activation against the pathogens. In the present study, we analyzed 14 A. thaliana accessions for natural variation in Pst-avrRps4 and C. higginsianum susceptibility, and found new compatible and incompatible Arabidopsis-pathogen interactions. We first found that A. thaliana accession Cvi-0 is susceptible to Pst-avrRps4. Interestingly, the genome sequence assembly indicated that Cvi-0 lost both RPS4 and RRS1, but not RPS4B and RRS1B, compared to the reference genome sequence from A. thaliana accession Col-0. On the other hand, the natural variation analysis of RPS4 alleles from various Arabidopsis accessions revealed that one amino-acid change, Y950H, is responsible for the loss of resistance to Pst-avrRps4 and C. higginsianum in RLD-0. Our data indicate that the amino acid change, Y950H, in RPS4 resulted in the loss of both RPS4 and RRS1 functions and resistance to pathogens.

  5. Chloroplast DNA trnQ-rps16 variation and genetic structure of nine wild Chinese cherry (Cerasus pseudocerasus Lindl.) populations%9个野生中国樱桃群体叶绿体 DNA trnQ-rps16序列变异及其遗传结构分析

    陈涛; 王小蓉; 罗华; 王春涛; 张家志; 罗明敏


    Chinese cherry (Cerasus pseudocerasus Lindl.) is one of the most economically domestic fruit trees in China. The rich variation of wild Chinese cherry is the most important breeding resource for existing cultivars. In order to reveal the levels and distribution of genetic variation within wild Chinese cherry of Sichuan Province, China, where is rich in wild Chinese cherry, the sequence variation of chloroplast DNA trnQ-rps16 intergenic spacer was analyzed in 145 individuals of all nine existing populations (seven from Sichuan, two from Shanxi and Guizhou provinces) of China. The results showed that trnQ-rps16 sequence were aligned with 13 polymorphic sites (1.87%), including 3 substitutions and 10 indels in 145 individuals, which revealed a low level of genetic diversity (h= 0.562,Π= 0.00184). Compared to other regions (h= 0.733, Π= 0.00243), a rather lower genetic diversity (h= 0.544,π= 0.00203) was found in the populations from Sichuan, and a large scale of genetic diversity among the seven populations was detected (h= 0-0.708; π= 0-0.00298), ranging from EM (h=0.000, Π=0.000) to TL (h=0.708, Π=0.00298). The low genetic diversity of populations may be strongly affected by founder effect and bottleneck effect because of the marginal nature, recent reduction, and consequent genetic drift of these populations. In addition, a fairly low genetic differentiation (FST= 0.21573) was found among the studied populations. This suggest that gene flow seems to originate from pronounced seed dispersal abilities of the species and it may play a significant role in shaping such a genetic structure. The long generation cycle of the species may also contribute to this structure. Based on these findings, a conservational plan for sampling or preserving fewer populations but more individuals from each population for the species was proposes.%中国樱桃(Cerasus pseudocerasus Lindl.)是我国古老的具有较高经济价值的栽培果树之一,个别性状突 出的野生

  6. RPS2 Proposal Submission Software: Testing and Distribution of Periodically Updated Software

    Douglas, R. E., Jr.


    In 1995, the Space Telescope Science Institute (STScI) introduced RPS2 (Remote Proposal Submission 2nd Generation). RPS2 is used by Hubble Space Telescope (HST) proposers to prepare their detailed observation descriptions. It is a client/server system implemented using Tcl/Tk. The client can transparently access servers on the user's machine, at STScI, or on any other machine on the Internet. The servers combine syntax checking, feasibility analysis, orbit packing, and constraint and schedulability analysis of user-specified observations. Prior to the release of RPS2, observers used a system which provided only syntax checking. RPS2 now provides the observers with some of the more advanced software, that had previously been available only to STScI staff for the preparation of detailed observing plans. The RPS2 system consists of four independent subsystems which are controlled by the client/server mechanism. A problem with a system of this size and complexity is that the software components, which continue to grow and change with HST itself, must continually be tested and distributed to those who need them. In the past, it had been acceptable to release the RPS2 software only once per observing cycle, but it became apparent before the 1997 HST Servicing Mission that multiple releases of RPS2 were going to be required to support the new instruments. This paper discusses how RPS2 and its component systems are maintained, updated, tested, and distributed.

  7. Antibodies against ribosomal protein S29 (RPS29) fused with glutathione's transferase specially react with native RPS29 in mouse and human cells

    Liu Jia; Zhang Junlei; Han Junfeng; Li Dongying; Jian Rui; Rao XianCai; Chen Wei; Wang Jiali; Xu Xiaofeng; Hu Zhen


    The ribosomal protein S29 also known as RPS29,is not only a component of the 40S subunit of ribosome,but also involved in embryonic development,oncogenesis and other pathologic conditions. However,rare commercial antibody against RPS29 restricts the discovery of precise physiological and pathological function of this protein. In this study,the whole RPS29 gene was inserted into plasmid pGEX-6p-1 to express glutathione's transferase (GST) fusion proteins in Escherichia coli (E. coli) strain BL21. High yields of soluble recombinant proteins were obtained. Mice were immunized with the recombinant RPS29 protein. The serum from the immunized mice could specially react with purified recombinant RPS29 proteins and native RPS29 proteins in CCE cells by western blotting,immunofluorescence staining and flow cytometric analysis. Further more the polyclonal antibodies also reacted specifically with human cell strain ECV304,which showed typical cytoplasmatic fluorescence. The polyclonal antibodies we prepared would be an available tool for studying the roles of RPS29 in embryonic development and human diseases.

  8. 3-RPS和3-SPR机构的工作空间差异分析%Analysis of Workspace Differences between 3-RPS and 3-SPR Mechanisms



    基于螺旋理论分别对3-RPS和3-SPR机构的自由度进行了分析 ,揭示了两机构由于动平台和静平台的倒置引起的运动转轴的位置变化.采用搜索法分别绘制了两机构的工作空间 ,揭示了转轴位置的差异引起的工作空间的不同.%The degrees of freedom of 3-RPS and 3-SPR are analyzed separately based on screw theory ,the changes of the movement shafts caused by the inversion of the moving platform and the fixed platform are pointed out .The workspaces of two mechanisms are mapped by the search method ,which concludes that different movement shaft will lead to the difference of the workspace .

  9. RPS27a promotes proliferation, regulates cell cycle progression and inhibits apoptosis of leukemia cells

    Wang, Houcai; Yu, Jing; Zhang, Lixia; Xiong, Yuanyuan; Chen, Shuying; Xing, Haiyan; Tian, Zheng; Tang, Kejing; Wei, Hui; Rao, Qing; Wang, Min; Wang, Jianxiang, E-mail:


    Highlights: • RPS27a expression was up-regulated in advanced-phase CML and AL patients. • RPS27a knockdown changed biological property of K562 and K562/G01 cells. • RPS27a knockdown affected Raf/MEK/ERK, P21 and BCL-2 signaling pathways. • RPS27a knockdown may be applicable for new combination therapy in CML patients. - Abstract: Ribosomal protein S27a (RPS27a) could perform extra-ribosomal functions besides imparting a role in ribosome biogenesis and post-translational modifications of proteins. The high expression level of RPS27a was reported in solid tumors, and we found that the expression level of RPS27a was up-regulated in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL) patients. In this study, we explored the function of RPS27a in leukemia cells by using CML cell line K562 cells and its imatinib resistant cell line K562/G01 cells. It was observed that the expression level of RPS27a was high in K562 cells and even higher in K562/G01 cells. Further analysis revealed that RPS27a knockdown by shRNA in both K562 and K562G01 cells inhibited the cell viability, induced cell cycle arrest at S and G2/M phases and increased cell apoptosis induced by imatinib. Combination of shRNA with imatinib treatment could lead to more cleaved PARP and cleaved caspase-3 expression in RPS27a knockdown cells. Further, it was found that phospho-ERK(p-ERK) and BCL-2 were down-regulated and P21 up-regulated in RPS27a knockdown cells. In conclusion, RPS27a promotes proliferation, regulates cell cycle progression and inhibits apoptosis of leukemia cells. It appears that drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might represent a novel therapy strategy in TKI resistant CML patients.

  10. The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences

    Bhattacharyya Madan K


    Full Text Available Abstract Background A series of Rps (resistance to Pytophthora sojae genes have been protecting soybean from the root and stem rot disease caused by the Oomycete pathogen, Phytophthora sojae. Five Rps genes were mapped to the Rps1 locus located near the 28 cM map position on molecular linkage group N of the composite genetic soybean map. Among these five genes, Rps1-k was introgressed from the cultivar, Kingwa. Rps1-k has been providing stable and broad-spectrum Phytophthora resistance in the major soybean-producing regions of the United States. Rps1-k has been mapped and isolated. More than one functional Rps1-k gene was identified from the Rps1-k locus. The clustering feature at the Rps1-k locus might have facilitated the expansion of Rps1-k gene numbers and the generation of new recognition specificities. The Rps1-k region was sequenced to understand the possible evolutionary steps that shaped the generation of Phytophthora resistance genes in soybean. Results Here the analyses of sequences of three overlapping BAC clones containing the 184,111 bp Rps1-k region are reported. A shotgun sequencing strategy was applied in sequencing the BAC contig. Sequence analysis predicted a few full-length genes including two Rps1-k genes, Rps1-k-1 and Rps1-k-2. Previously reported Rps1-k-3 from this genomic region 1 was evolved through intramolecular recombination between Rps1-k-1 and Rps1-k-2 in Escherichia coli. The majority of the predicted genes are truncated and therefore most likely they are nonfunctional. A member of a highly abundant retroelement, SIRE1, was identified from the Rps1-k region. The Rps1-k region is primarily composed of repetitive sequences. Sixteen simple repeat and 63 tandem repeat sequences were identified from the locus. Conclusion These data indicate that the Rps1 locus is located in a gene-poor region. The abundance of repetitive sequences in the Rps1-k region suggested that the location of this locus is in or near a

  11. Pervasive survival of expressed mitochondrial rps14 pseudogenes in grasses and their relatives for 80 million years following three functional transfers to the nucleus

    Palmer Jeffrey D


    Full Text Available Abstract Background Many mitochondrial genes, especially ribosomal protein genes, have been frequently transferred as functional entities to the nucleus during plant evolution, often by an RNA-mediated process. A notable case of transfer involves the rps14 gene of three grasses (rice, maize, and wheat, which has been relocated to the intron of the nuclear sdh2 gene and which is expressed and targeted to the mitochondrion via alternative splicing and usage of the sdh2 targeting peptide. Although this transfer occurred at least 50 million years ago, i.e., in a common ancestor of these three grasses, it is striking that expressed, nearly intact pseudogenes of rps14 are retained in the mitochondrial genomes of both rice and wheat. To determine how ancient this transfer is, the extent to which mitochondrial rps14 has been retained and is expressed in grasses, and whether other transfers of rps14 have occurred in grasses and their relatives, we investigated the structure, expression, and phylogeny of mitochondrial and nuclear rps14 genes from 32 additional genera of grasses and from 9 other members of the Poales. Results Filter hybridization experiments showed that rps14 sequences are present in the mitochondrial genomes of all examined Poales except for members of the grass subfamily Panicoideae (to which maize belongs. However, PCR amplification and sequencing revealed that the mitochondrial rps14 genes of all examined grasses (Poaceae, Cyperaceae, and Joinvilleaceae are pseudogenes, with all those from the Poaceae sharing two 4-NT frameshift deletions and all those from the Cyperaceae sharing a 5-NT insertion (only one member of the Joinvilleaceae was examined. cDNA analysis showed that all mitochondrial pseudogenes examined (from all three families are transcribed, that most are RNA edited, and that surprisingly many of the edits are reverse (U→C edits. Putatively nuclear copies of rps14 were isolated from one to several members of each of these

  12. Diverse forms of RPS9 splicing are part of an evolving autoregulatory circuit.

    Alex M Plocik

    Full Text Available Ribosomal proteins are essential to life. While the functions of ribosomal protein-encoding genes (RPGs are highly conserved, the evolution of their regulatory mechanisms is remarkably dynamic. In Saccharomyces cerevisiae, RPGs are unusual in that they are commonly present as two highly similar gene copies and in that they are over-represented among intron-containing genes. To investigate the role of introns in the regulation of RPG expression, we constructed 16 S. cerevisiae strains with precise deletions of RPG introns. We found that several yeast introns function to repress rather than to increase steady-state mRNA levels. Among these, the RPS9A and RPS9B introns were required for cross-regulation of the two paralogous gene copies, which is consistent with the duplication of an autoregulatory circuit. To test for similar intron function in animals, we performed an experimental test and comparative analyses for autoregulation among distantly related animal RPS9 orthologs. Overexpression of an exogenous RpS9 copy in Drosophila melanogaster S2 cells induced alternative splicing and degradation of the endogenous copy by nonsense-mediated decay (NMD. Also, analysis of expressed sequence tag data from distantly related animals, including Homo sapiens and Ciona intestinalis, revealed diverse alternatively-spliced RPS9 isoforms predicted to elicit NMD. We propose that multiple forms of splicing regulation among RPS9 orthologs from various eukaryotes operate analogously to translational repression of the alpha operon by S4, the distant prokaryotic ortholog. Thus, RPS9 orthologs appear to have independently evolved variations on a fundamental autoregulatory circuit.

  13. Regional REC and RPS Best Practices

    Jennifer Alvarado


    The Great Lakes Renewable Energy Association conducted a program to explore the development of Renewable Energy Portfolio Standards and Renewable Energy Certificate Markets in the Midwest. The initiative represented the collaboration between the four state energy offices of Illinois, Indiana, Michigan and Ohio, the Great Lakes Renewable Energy Association (GLREA) and the Clean Energy State Alliance (CESA). The multi-state project explored the opportunities in the Midwest to expand the renewable energy market through Renewable Energy Portfolio Standards (RPS) and the trading of Renewable Energy Credits (RECs).

  14. Lineage-specific group II intron gains and losses of the mitochondrial rps3 gene in gymnosperms.

    Regina, Teresa M R; Quagliariello, Carla


    According to PCR assays and sequencing, we now report the shared presence of two rps3 introns, namely the rps3i74 and the rps3i249, in the mitochondria of all the classes representing the surviving lineages of gymnosperms, and unveil several lineages experiencing intron loss. Interestingly, the rps3 intron gains and losses within the four groups of gymnosperms let us sort out the Pinaceae and the non-Pinaceae into intron (+)- and intron (-)-lineages, respectively. Worthy of mention is also the finding that only Gnetum within the Gnetales harbours both the rps3 introns. This intron distribution pattern is consistent with the hypothesis that the two rps3 introns were likely present in the common ancestor of the seed plants and, then, independently lost in the non-Pinaceae during gymnosperm evolution. The derived secondary structural model of the novel group IIA intron improves our understanding of the significance and origin of the extraordinary length polymorphisms observed among rps3i249 orthologs. Despite the remarkable structural plasticity to adopt and reject introns, the rps3 mRNAs undergo accurate processing by splicing and extensive editing in gymnosperm mitochondria. This study provides additional insights into the evolutionarily high dynamics of mitochondrial introns which may come and go in closely related plant species. The turnover of the mitochondrial rps3 group II introns seen among lineages of seed plants further suggests that these introns might be an additional signature to discriminate between particularly cryptical taxonomic groups for which there is a need of a further evaluation of their evolutionary affiliation.

  15. A homolog of the RPS2 disease resistance gene is constitutively expressed in Brassica oleracea

    Malvas Celia C.


    Full Text Available In this study, we identified disease resistance gene homologs in Brassica oleracea and assessed their expression in lines resistant and susceptible to Xanthomonas campestris pv. campestris (Xcc. Two DNA fragments of approximately 2.5 kb (BI-16/RPS2 and Lc201/RPS2 were amplified by PCR from two Brassica lines using primers based on an RPS2 homologous sequence previously described in the Brassica oleracea ecotype B117. The sequences of these fragments shared high similarity (95-98% with RPS2 homologs from various Brassica species. The digestion of these fragments with restriction enzymes revealed polymorphisms at the Xba I restriction sites. The length polymorphisms were used as a co-dominant marker in an F2 population developed to segregate for resistance to Xcc, the causal agent of black rot. Linkage analysis showed no significant association between the marker and quantitative trait loci for black rot. RT-PCR with specific primers yielded an expected 453 bp fragment that corresponded to the RPS2 homologs in both resistant and susceptible lines inoculated with the pathogen, as well as in non-inoculated control plants. These results suggest that these homologs are constitutively expressed in B. oleracea.

  16. RPS8--a new informative DNA marker for phylogeny of Babesia and Theileria parasites in China.

    Zhan-Cheng Tian

    Full Text Available Piroplasmosis is a serious debilitating and sometimes fatal disease. Phylogenetic relationships within piroplasmida are complex and remain unclear. We compared the intron-exon structure and DNA sequences of the RPS8 gene from Babesia and Theileria spp. isolates in China. Similar to 18S rDNA, the 40S ribosomal protein S8 gene, RPS8, including both coding and non-coding regions is a useful and novel genetic marker for defining species boundaries and for inferring phylogenies because it tends to have little intra-specific variation but considerable inter-specific difference. However, more samples are needed to verify the usefulness of the RPS8 (coding and non-coding regions gene as a marker for the phylogenetic position and detection of most Babesia and Theileria species, particularly for some closely related species.

  17. Implications of Scheduled ITC Reversion for RPS Compliance: Preliminary Results

    Lowder, Travis; Miller, John; O' Shaughnessy, Eric; Heeter, Jenny


    This poster presents DRAFT initial results of a forthcoming NREL analysis. The analysis investigates the impacts of the scheduled investment tax credit (ITC) reversion from 30 percent to 10 percent for certain solar photovoltaic projects. Specifically, it considers whether the reversion will result in increased use of alternative compliance payments (ACPs) in lieu of solar renewable energy credits (SRECs) for renewable portfolio standard (RPS) compliance. The analysis models the effect of a 10 percent ITC on power purchase agreement (PPA) prices for non-residential systems in the eight states with solar carve-outs and solar ACPs. Our preliminary results suggest that states will likely install sufficient capacity to meet long-term targets through SRECs rather than ACPs following the ITC reversion. However, the analysis shows that the ITC reversion could affect project economics such that capacity shortfalls in certain states could temporarily increase the use of ACPs. NREL anticipates publishing a full report of this analysis in fall 2015. credits (SRECs) for renewable portfolio standard (RPS) compliance. The analysis models the effect of a 10 percent ITC on power purchase agreement (PPA) prices for non-residential systems in the eight states with solar carve-outs and solar ACPs. Our preliminary results suggest that states will likely install sufficient capacity to meet long-term targets through SRECs rather than ACPs following the ITC reversion. However, the analysis shows that the ITC reversion could affect project economics such that capacity shortfalls in certain states could temporarily increase the use of ACPs. NREL anticipates publishing a full report of this analysis in fall 2015.

  18. Implications of Scheduled ITC Reversion for RPS Compliance: Preliminary Results

    Lowder, Travis; Miller, John; O' Shaughnessy, Eric; Heeter, Jenny


    This poster presents DRAFT initial results of a forthcoming NREL analysis. The analysis investigates the impacts of the scheduled investment tax credit (ITC) reversion from 30 percent to 10 percent for certain solar photovoltaic projects. Specifically, it considers whether the reversion will result in increased use of alternative compliance payments (ACPs) in lieu of solar renewable energy credits (SRECs) for renewable portfolio standard (RPS) compliance. The analysis models the effect of a 10 percent ITC on power purchase agreement (PPA) prices for non-residential systems in the eight states with solar carve-outs and solar ACPs. Our preliminary results suggest that states will likely install sufficient capacity to meet long-term targets through SRECs rather than ACPs following the ITC reversion. However, the analysis shows that the ITC reversion could affect project economics such that capacity shortfalls in certain states could temporarily increase the use of ACPs. NREL anticipates publishing a full report of this analysis in fall 2015. credits (SRECs) for renewable portfolio standard (RPS) compliance. The analysis models the effect of a 10 percent ITC on power purchase agreement (PPA) prices for non-residential systems in the eight states with solar carve-outs and solar ACPs. Our preliminary results suggest that states will likely install sufficient capacity to meet long-term targets through SRECs rather than ACPs following the ITC reversion. However, the analysis shows that the ITC reversion could affect project economics such that capacity shortfalls in certain states could temporarily increase the use of ACPs. NREL anticipates publishing a full report of this analysis in fall 2015.

  19. Molecular analysis of TP53, D9S1747, D9S162 and RPS6 in oral squamous cell carcinoma%微卫星位点TP53、D9S1747、D9S162及RPS6的杂合性缺失与口腔鳞状细胞癌的关系

    王新红; 张颖颖; 陈新明; 孙志军


    Objective To investigate the correlation between the frequency of molecular abnormalities of 4 loci at chromosomal 9p21 (D9S1747 ,D9S162, RPS6) and 17p13 (TP53) and the clinical characteristics and prognosis. Methods The oral squamous cell carcinoma(OSCC) lesions in 71 patients were manually microdessected. Genomic DNA from these lesions and normal lymphnode tissu or peripheral blood of the same patients were extracted using the Watson's tissue kit. The loss of heterozygosity(LOH) and microsatellite instability (MI) of 17p13 and 9p21 were analyzed by PCR-page electrophoresis after DNA extraction. Results LOH and MI were detected in the OSCC of 48 patients (68%). The LOH and MI frequency at chromosomes 17p13 and 9P21 were 56% (35/63) and 59% (40/68) respectively. The LOH and MI frequency at 9p21 was significantly associated with WHO grading ( P < 0.01 ) and lymphonode metastasis (P <0.01 ). The LOH and MI frequency at 17p13 was significantly associated with clinical stage (P < 0.05 ). TP53 genetic aberration and 9p21 genetic aberration were significant prognostic factors for OSCC. The prognosis was poor in the LOH and MI positive group of chromosome 17p13 and 9p21. The frequency of LOH and MI at TP53 was the only independent factor for overall survival ( P < 0.05 ).Conclusions The LOH and MI of 17p13 and 9p21 were related to clinical stage and lymphonode metastasis. LOH of TP53 was an independent prognostic factor for OSCC.%目的 检测微卫星位点TP53(位于17p13区)、D9S1747、D9S162、RPS6(均位于9p21区)在口腔鳞状细胞癌中的杂合性缺失(1oss of heterozygosity,LOH)和微卫星不稳定(microsatellite instability,MI)与临床病理因素及预后的关系.方法 取71例符合纳入标准的口腔鳞状细胞癌病变组织,用基因组DNA快速提取纯化试剂盒提取肿瘤组织及对应正常淋巴结或外周血的DNA,聚合酶链反应(PCR)变性电泳检测上述位点的基因变化.结果 所有位点在癌组

  20. Fast evolution of the retroprocessed mitochondrial rps3 gene in Conifer II and further evidence for the phylogeny of gymnosperms.

    Ran, Jin-Hua; Gao, Hui; Wang, Xiao-Quan


    The popular view that plant mitochondrial genome evolves slowly in sequence has been recently challenged by the extraordinarily high substitution rates of mtDNA documented mainly from several angiosperm genera, but high substitution rate acceleration accompanied with great length variation has been very rarely reported in plant mitochondrial genes. Here, we studied evolution of the mitochondrial rps3 gene that encodes the ribosomal small subunit protein 3 and found a dramatically high variation in both length and sequence of an exon region of it in Conifer II. A sequence comparison between cDNA and genomic DNA showed that there are no RNA editing sites in the Conifer II rps3 gene. Southern blotting analyses of the total DNA and mtDNA, together with the real-time PCR analysis, showed that rps3 exists as a single mitochondrial locus in gymnosperms. It is very likely that the Conifer II rps3 gene has experienced retroprocessing, i.e., the re-integration of its cDNA into the mitochondrial genome, followed by an evolutionary acceleration due to the intron loss. In addition, the phylogenetic analysis of rps3 supports the sister relationship between conifers and Gnetales. In particular, the monophyly of conifer II is strongly supported by the shared loss of two rps3 introns. Our results also indicate that the mitochondrial gene tree would be affected in topology when the "edited" paralogs are analyzed together with their genomic sequences.

  1. Characterization of local complex structures in a recurrence plot to improve nonlinear dynamic discriminant analysis.

    Ding, Hang


    Structures in recurrence plots (RPs), preserving the rich information of nonlinear invariants and trajectory characteristics, have been increasingly analyzed in dynamic discrimination studies. The conventional analysis of RPs is mainly focused on quantifying the overall diagonal and vertical line structures through a method, called recurrence quantification analysis (RQA). This study extensively explores the information in RPs by quantifying local complex RP structures. To do this, an approach was developed to analyze the combination of three major RQA variables: determinism, laminarity, and recurrence rate (DLR) in a metawindow moving over a RP. It was then evaluated in two experiments discriminating (1) ideal nonlinear dynamic series emulated from the Lorenz system with different control parameters and (2) data sets of human heart rate regulations with normal sinus rhythms (n = 18) and congestive heart failure (n = 29). Finally, the DLR was compared with seven major RQA variables in terms of discriminatory power, measured by standardized mean difference (DSMD). In the two experiments, DLR resulted in the highest discriminatory power with DSMD = 2.53 and 0.98, respectively, which were 7.41 and 2.09 times the best performance from RQA. The study also revealed that the optimal RP structures for the discriminations were neither typical diagonal structures nor vertical structures. These findings indicate that local complex RP structures contain some rich information unexploited by RQA. Therefore, future research to extensively analyze complex RP structures would potentially improve the effectiveness of the RP analysis in dynamic discrimination studies.

  2. Characterization of local complex structures in a recurrence plot to improve nonlinear dynamic discriminant analysis

    Ding, Hang


    Structures in recurrence plots (RPs), preserving the rich information of nonlinear invariants and trajectory characteristics, have been increasingly analyzed in dynamic discrimination studies. The conventional analysis of RPs is mainly focused on quantifying the overall diagonal and vertical line structures through a method, called recurrence quantification analysis (RQA). This study extensively explores the information in RPs by quantifying local complex RP structures. To do this, an approach was developed to analyze the combination of three major RQA variables: determinism, laminarity, and recurrence rate (DLR) in a metawindow moving over a RP. It was then evaluated in two experiments discriminating (1) ideal nonlinear dynamic series emulated from the Lorenz system with different control parameters and (2) data sets of human heart rate regulations with normal sinus rhythms (n = 18) and congestive heart failure (n = 29). Finally, the DLR was compared with seven major RQA variables in terms of discriminatory power, measured by standardized mean difference (DSMD). In the two experiments, DLR resulted in the highest discriminatory power with DSMD = 2.53 and 0.98, respectively, which were 7.41 and 2.09 times the best performance from RQA. The study also revealed that the optimal RP structures for the discriminations were neither typical diagonal structures nor vertical structures. These findings indicate that local complex RP structures contain some rich information unexploited by RQA. Therefore, future research to extensively analyze complex RP structures would potentially improve the effectiveness of the RP analysis in dynamic discrimination studies.

  3. NASA's Radioisotope Power Systems Program Overview - A Focus on RPS Users

    Hamley, John A.; McCallum, Peter W.; Sandifer, Carl E., II; Sutliff, Thomas J.; Zakrajsek, June F.


    The goal of NASA's Radioisotope Power Systems (RPS) Program is to make RPS ready and available to support the exploration of the solar system in environments where the use of conventional solar or chemical power generation is impractical or impossible to meet potential future mission needs. To meet this goal, the RPS Program manages investments in RPS technologies and RPS system development, working closely with the Department of Energy. This paper provides an overview of the RPS Program content and status, its collaborations with potential RPS users, and the approach employed to maintain the readiness of RPS to support future NASA mission concepts.

  4. The beta hairpin structure within ribosomal protein S5 mediates interplay between domains II and IV and regulates HCV IRES function.

    Bhat, Prasanna; Shwetha, Shivaprasad; Sharma, Divya Khandige; Joseph, Agnel Praveen; Srinivasan, Narayanaswamy; Das, Saumitra


    Translation initiation in Hepatitis C Virus (HCV) is mediated by Internal Ribosome Entry Site (IRES), which is independent of cap-structure and uses a limited number of canonical initiation factors. During translation initiation IRES-40S complex formation depends on high affinity interaction of IRES with ribosomal proteins. Earlier, it has been shown that ribosomal protein S5 (RPS5) interacts with HCV IRES. Here, we have extensively characterized the HCV IRES-RPS5 interaction and demonstrated its role in IRES function. Computational modelling and RNA-protein interaction studies demonstrated that the beta hairpin structure within RPS5 is critically required for the binding with domains II and IV. Mutations disrupting IRES-RPS5 interaction drastically reduced the 80S complex formation and the corresponding IRES activity. Computational analysis and UV cross-linking experiments using various IRES-mutants revealed interplay between domains II and IV mediated by RPS5. In addition, present study demonstrated that RPS5 interaction is unique to HCV IRES and is not involved in 40S-3' UTR interaction. Further, partial silencing of RPS5 resulted in preferential inhibition of HCV RNA translation. However, global translation was marginally affected by partial silencing of RPS5. Taken together, results provide novel molecular insights into IRES-RPS5 interaction and unravel its functional significance in mediating internal initiation of translation.

  5. A Novel Phytophthora sojae Resistance Rps12 Gene Mapped to a Genomic Region That Contains Several Rps Genes

    Sahoo, Dipak K.; Abeysekara, Nilwala S.; Cianzio, Silvia R.; Robertson, Alison E.


    Phytophthora sojae Kaufmann and Gerdemann, which causes Phytophthora root rot, is a widespread pathogen that limits soybean production worldwide. Development of Phytophthora resistant cultivars carrying Phytophthora resistance Rps genes is a cost-effective approach in controlling this disease. For this mapping study of a novel Rps gene, 290 recombinant inbred lines (RILs) (F7 families) were developed by crossing the P. sojae resistant cultivar PI399036 with the P. sojae susceptible AR2 line, and were phenotyped for responses to a mixture of three P. sojae isolates that overcome most of the known Rps genes. Of these 290 RILs, 130 were homozygous resistant, 12 heterzygous and segregating for Phytophthora resistance, and 148 were recessive homozygous and susceptible. From this population, 59 RILs homozygous for Phytophthora sojae resistance and 61 susceptible to a mixture of P. sojae isolates R17 and Val12-11 or P7074 that overcome resistance encoded by known Rps genes mapped to Chromosome 18 were selected for mapping novel Rps gene. A single gene accounted for the 1:1 segregation of resistance and susceptibility among the RILs. The gene encoding the Phytophthora resistance mapped to a 5.8 cM interval between the SSR markers BARCSOYSSR_18_1840 and Sat_064 located in the lower arm of Chromosome 18. The gene is mapped 2.2 cM proximal to the NBSRps4/6-like sequence that was reported to co-segregate with the Phytophthora resistance genes Rps4 and Rps6. The gene is mapped to a highly recombinogenic, gene-rich genomic region carrying several nucleotide binding site-leucine rich repeat (NBS-LRR)-like genes. We named this novel gene as Rps12, which is expected to be an invaluable resource in breeding soybeans for Phytophthora resistance. PMID:28081566

  6. Feed-in Tariff Policy: Design, Implementation, and RPS Policy Interactions

    Cory, K.; Couture, T.; Kreycik, C.


    Feed-in tariff (FIT) policies are implemented in more than 40 countries around the world and are cited as the primary reason for the success of the German and Spanish renewable energy markets. As a result of that success, FIT policy proposals are starting to gain traction in several U.S. states and municipalities. Experience from Europe is also beginning to demonstrate that properly designed FITs may be more cost-effective than renewable portfolio standards (RPS), which make use of competitive solicitations. This article explores the design and operation of feed-in tariff policies, including a FIT policy definition, payment-structure options, and payment differentiation. The article also touches on the potential interactions between FIT policies and RPS policies at the state level.

  7. Molecular network including eIF1AX, RPS7, and 14-3-3γ regulates protein translation and cell proliferation in bovine mammary epithelial cells.

    Yu, Cuiping; Luo, Chaochao; Qu, Bo; Khudhair, Nagam; Gu, Xinyu; Zang, Yanli; Wang, Chunmei; Zhang, Na; Li, Qingzhang; Gao, Xuejun


    14-3-3γ, an isoform of the 14-3-3 protein family, was proved to be a positive regulator of mTOR pathway. Here, we analyzed the function of 14-3-3γ in protein synthesis using bovine mammary epithelial cells (BMECs). We found that 14-3-3γ interacted with eIF1AX and RPS7 by 14-3-3γ coimmunoprecipitation (CoIP) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) peptide mass fingerprinting analysis. These interactions of 14-3-3γ with eIF1AX and RPS7 were further confirmed by colocalization and fluorescence resonance energy transfer (FRET) analysis. We also found that methionine could promote protein synthesis and trigger the protein expression levels of 14-3-3γ, eIF1AX and RPS7. Analysis of overexpression and inhibition of 14-3-3γ confirmed that it positively affected the protein expression levels of eIF1AX, RPS7, Stat5 and mTOR pathway to promote protein synthesis and cell proliferation in BMECs. We further showed that overexpression of eIF1AX and RPS7 also triggered protein translation and cell proliferation. From these results, we conclude that molecular network including eIF1AX, RPS7, and 14-3-3γ regulates protein translation and cell proliferation in BMECs.

  8. Expression of RPS4 in tobacco induces an AvrRps4-independent HR that requires EDS1, SGT1 and HSP90.

    Zhang, Yan; Dorey, Stephan; Swiderski, Michal; Jones, Jonathan D G


    The Arabidopsis RPS4 gene belongs to the Toll/interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR-NB-LRR) class of plant resistance (R) genes. It confers resistance to Pseudomonas syringae carrying the avirulence gene avrRps4. Transient expression of genomic RPS4 driven by the 35S promoter in tobacco leaves induces an AvrRps4-independent hypersensitive response (HR). The same phenotype is seen after expression of a full-length RPS4 cDNA. This indicates that alternative splicing of RPS4 is not involved in this HR. The extent of HR is correlated with RPS4 protein levels. Deletion analyses of RPS4 domains show the TIR domain is required for the HR phenotype. Mutations in the P-loop motif of the NB domain abolish the HR. Using virus-induced gene silencing, we found that the cell death resulting from RPS4 expression is dependent on the three plant signalling components EDS1, SGT1 and HSP90. All these data suggest that heterologous expression of an R gene can result in activation of cell death even in the absence of its cognate avirulence product, and provides a system for studying the RPS4 domains required for HR.

  9. Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1.

    Narusaka, Mari; Toyoda, Kazuhiro; Shiraishi, Tomonori; Iuchi, Satoshi; Takano, Yoshitaka; Shirasu, Ken; Narusaka, Yoshihiro


    Arabidopsis thaliana leucine-rich repeat-containing (NLR) proteins RPS4 and RRS1, known as dual resistance proteins, confer resistance to multiple pathogen isolates, such as the bacterial pathogens Pseudomonas syringae and Ralstonia solanacearum and the fungal pathogen Colletotrichum higginsianum. RPS4 is a typical Toll/interleukin 1 Receptor (TIR)-type NLR, whereas RRS1 is an atypical TIR-NLR that contains a leucine zipper (LZ) motif and a C-terminal WRKY domain. RPS4 and RRS1 are localised near each other in a head-to-head orientation. In this study, direct mutagenesis of the C-terminal LZ motif in RRS1 caused an autoimmune response and stunting in the mutant. Co-immunoprecipitation analysis indicated that full-length RPS4 and RRS1 are physically associated with one another. Furthermore, virus-induced gene silencing experiments showed that hypersensitive-like cell death triggered by RPS4/LZ motif-mutated RRS1 depends on EDS1. In conclusion, we suggest that the RRS1-LZ motif is crucial for the regulation of the RPS4/RRS1 complex.

  10. Investigation of Insulation Materials for Future Radioisotope Power Systems (RPS)

    Cornell, Peggy A.; Hurwitz, Frances I.; Ellis, David L.; Schmitz, Paul C.


    NASA's Radioisotope Power System (RPS) Technology Advancement Project is developing next generation high temperature insulation materials that directly benefit thermal management and improve performance of RPS for future science missions. Preliminary studies on the use of multilayer insulation (MLI) for Stirling convertors used on the Advanced Stirling Radioisotope Generator (ASRG) have shown the potential benefits of MLI for space vacuum applications in reducing generator size and increasing specific power (W/kg) as compared to the baseline Microtherm HT (Microtherm, Inc.) insulation. Further studies are currently being conducted at NASA Glenn Research Center (GRC) on candidate MLI foils and aerogel composite spacers. This paper presents the method of testing of foils and spacers and experimental results to date.

  11. AMTEC radioisotope power system design and analysis for Pluto Express Fly-By

    Hendricks, T.J.; Huang, C.; Sievers, R.K. [Advanced Modular Power Systems, Inc., Ann Arbor, MI (United States)


    The Pluto Express Fly-By program requires a Radioisotope Power System (RPS) to supply spacecraft power for various internal functions and mission instruments and experiments. AMTEC (Alkali-Metal Thermal-Electric Conversion) power conversion is the DOE-selected technology for an advanced, high-efficiency RPS to power the Pluto Express Fly-By spacecraft. An AMTEC-based RPS using the General Purpose Heat Source (GPHS) has been conceptually designed to satisfy the Pluto Express power requirements. Integrated AMTEC cell and system thermal/electrical design analyses, structural design analyses, and mass analyses were performed to define an optimum system design. Using fresh radioisotope fuel at beginning of mission, the RPS produces 102 watts of power, has a mass of 8.35 kg (specific power density = 12.2 watts/kg), with a system conversion efficiency of 20.3%. Mass/power scale-up estimates have also been generated, indicating that a 150-watt version of this RPS would weigh approximately 11.3 kg. This paper presents and discusses the key features of this RPS design, the design and analysis methodology, and the numerous system and AMTEC cell tradeoff studies establishing the optimum AMTEC-based RPS.

  12. Diamond-Blackfan anemia with mandibulofacial dystostosis is heterogeneous, including the novel DBA genes TSR2 and RPS28.

    Gripp, Karen W; Curry, Cynthia; Olney, Ann Haskins; Sandoval, Claudio; Fisher, Jamie; Chong, Jessica Xiao-Ling; Pilchman, Lisa; Sahraoui, Rebecca; Stabley, Deborah L; Sol-Church, Katia


    Patients with physical findings suggestive of Treacher Collins syndrome (TCS) or mandibulofacial dysostosis (MFD) and macrocytic anemia diagnostic of Diamond-Blackfan anemia (DBA) have been reported. Disease-causing genes have been identified for TCS and other MFDs. Mutations in several ribosomal protein genes and the transcription factor GATA1 result in DBA. However, no disease-causing mutation had been identified in the reported patients with the combination of TCS/MFD and DBA phenotype, and we hypothesized that pathogenic mutations in a single gene could be identified using whole exome analysis. We studied probands from six unrelated families. Combining exome analysis and Sanger sequencing, we identified likely pathogenic mutations in 5/6 families. Two mutations in unrelated families were seen in RPS26, the known DBA10 gene. One variant was predicted to affect mRNA splicing, and the other to lead to protein truncation. In another family a likely pathogenic X-linked mutation affecting a highly conserved residue was found in TSR2, which encodes a direct binding partner of RPS26. De novo mutations affecting the RPS28 start codon were found in two unrelated probands, identifying RPS28 as a novel disease gene. We conclude that the phenotype combining features of TCS with DBA is genetically heterogeneous. Each of the pathogenic variants identified is predicted to impede ribosome biogenesis, which in turn could result in altered cell growth and proliferation, causing abnormal embryologic development, defective erythropoiesis and reduced growth. The phenotype combining TCS/MFD and DBA is highly variable, overlaps with DBA and lies within the phenotypic spectrum of ribosomopathies. © 2014 Wiley Periodicals, Inc.

  13. Application of rps16 Intron and trnL-trnF Intergenic Spacer Sequences to Identify Rengas Clone Riau

    Dewi Indriyani Roslim


    Full Text Available Rengas clone Riau has been identified using morphological characters and molecular technique with a psbA-trnH intergenic spacer, however, this method can only determine its taxonomic status at genus level, namely Gluta sp.  This study reports application two DNA barcodes, i.e. rps16 intron and trnL-trnF intergenic spacer, to identify Rengas clone Riau.  The methods included collection of the leaves from Kajuik Lake, total DNA isolation, electrophoresis, PCR (polymerase chain reaction, gel purification and sequencing.  The rps16 intron size was 659 bp and the trnL-trnF intergenic spacer was 527 bp. The BLASTn analysis showed that sequences of the rps16 intron and the trnL-trnF intergenic spacer of Gluta sp clone Riau had 100% similarity to those of G. renghas deposited in GenBank.  These results were supported by high max score, high total score, query cover = 100%, and E-value = 0.  The dendrograms also showed the closest relationship of Gluta sp clone Riau with G. renghas deposited in GenBank compared to other species of Gluta.  In conclusion, this study succeeded in identifying Rengas clone Riau as Gluta renghas by using sequences of the rps16 intron and the trnL-trnF intergenic spacer. A combination of DNA barcodes could be applied to identify various plants as long as the database for the DNA barcodes is available in public database such as GenBank. 

  14. Advanced Stirling Convertor (ASC) Development for NASA RPS

    Wong, Wayne A.; Wilson, Scott; Collins, Josh


    Sunpower's Advanced Stirling Convertor (ASC) initiated development under contract to the NASA Glenn Research Center (GRC) and after a series of successful demonstrations, the ASC began transitioning from a technology development project to flight development project. The ASC has very high power conversion efficiency making it attractive for future Radioisotope Power Systems (RPS) in order to make best use of the low plutonium-238 fuel inventory in the U.S. In recent years, the ASC became part of the NASA-Department of Energy Advanced Stirling Radioisotope Generator (ASRG) Integrated Project. Sunpower held two parallel contracts to produce ASC convertors, one with the Department of Energy/Lockheed Martin to produce the ASC-F flight convertors, and one with NASA GRC for the production of ASC-E3 engineering units, the initial units of which served as production pathfinders. The integrated ASC technical team successfully overcame various technical challenges that led to the completion and delivery of the first two pairs of flight-like ASC-E3 by 2013. However, in late Fall 2013, the DOE initiated termination of the Lockheed Martin ASRG flight development contract driven primarily by budget constraints. NASA continues to recognize the importance of high efficiency ASC power conversion for RPS and continues investment in the technology including the continuation of ASC-E3 production at Sunpower and the assembly of the ASRG Engineering Unit #2. This paper provides a summary of ASC technical accomplishments, overview of tests at GRC, plans for continued ASC production at Sunpower, and status of Stirling technology development.

  15. Released polysaccharides (RPS) from Cyanothece sp. CCY 0110 as biosorbent for heavy metals bioremediation: interactions between metals and RPS binding sites.

    Mota, Rita; Rossi, Federico; Andrenelli, Luisa; Pereira, Sara Bernardes; De Philippis, Roberto; Tamagnini, Paula


    Bioremediation of heavy metals using microorganisms can be advantageous compared to conventional physicochemical methods due to the use of renewable resources and efficiencies of removal particularly cations at low concentrations. In this context, cyanobacteria/cyanobacterial extracellular polymeric substances (EPS) emerge as a valid alternative due to the anionic nature and particular composition of these polymers. In this work, various culture fractions of the unicellular cyanobacterium Cyanothece sp. CCY 0110 were employed in bioremoval assays using three of the most common heavy metal pollutants in water bodies-copper, cadmium, and lead-separately or in combined systems. Our study showed that the released polysaccharides (RPS) were the most efficient fraction, removing the metal(s) by biosorption. Therefore, this polymer was subsequently used to evaluate the interactions between the metals/RPS binding sites using SEM-EDX, ICP-OES, and FTIR. Acid and basic pretreatments applied to the polymer further improve the process efficiency, and the exposure to an alkaline solution seems to alter the RPS conformation. The differences observed in the specific metal bioremoval seem to be mainly due to the RPS organic functional groups available, mainly carboxyl and hydroxyl, than to an ion exchange mechanism. Considering that Cyanothece is a highly efficient RPS-producer and that RPS can be easily separated from the culture, immobilized or confined, this polymer can be advantageous for the establishment/improvement of heavy metal removal systems.

  16. 先天性纯红细胞再生障碍性贫血斑马鱼模型相关microRNA的组学研究%microRNome analysis of the Zebrafish model of diamond-blackfan anemia from Rps19 deficiency

    张倩; 贾海波; 方向东; 宋斌峰; 万扬; 张昭军; 鲍丙浩; 谢尚论; 刘汉芝; 竺晓凡; 袁卫平


    目的:探讨先天性纯红细胞再生障碍性贫血(DBA)相关 microRNA 的表达特征及其调控机制。方法利用 morpholino 技术建立核糖体蛋白基因 Rps19表达不足的 DBA 斑马鱼模型和Rps19与 p53同时表达不足的斑马鱼模型,利用新一代高通量测序(RNA-seq)技术,研究 DBA 斑马鱼模型的 microRNA 表达谱的特征及 p53在其中的调控作用。结果Rps19 MO 的斑马鱼胚胎的循环血细胞明显减少,且尾部呈现出向腹侧弯曲的异常形态。而在 p53敲低之后,斑马鱼的形态异常得到了一定程度的恢复。通过对 microRNA 表达谱的比较分析发现,在 Rps19不足的斑马鱼胚胎中约1/4的 microRNA 表达量显著偏低,这些异常表达的 microRNA 可能影响到斑马鱼的正常发育过程。而在 p53敲低之后,部分 microRNA 的表达恢复到正常水平。最终确定了47个在 DBA 发病过程中可能受 p53调控的 microRNA。结论DBA 斑马鱼模型的 microRNA 普遍低表达,其中部分 microRNA 受到 p53的表达水平的调控。%Objective To characterize the genome-wide microRNA profile and to explore the biological functions and probable mechanisms for microRNAs in diamond-blackfan anemia (DBA). Method Zebrafish embryos at the one-cell stage were injected with the Rps19 MO and Rps19+p53 MO. The miRNomes of these zebrafish models and control, generated by miRNA-seq, were compared for determining the microRNA profile characteristics of DBA and regulatory function of p53. Result Rps19 morphants showed an obviously ventrally bent tail and a reduction in the circulating blood cells compared with the control embryos, which were partially rescued in Rps19+p53 MO at 48 hpf. By comparing the miRNomes with control, we found that about 25% of total expressed microRNAs in Rps19 MO had significantly lower expression, which might make an influence on the development process of Zebrafish embryos. The abnormal miRNomes were partially

  17. Development of RPS trip logic based on PLD technology

    Choi, Jong Gyun; Lee, Dong Young [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)


    The majority of instrumentation and control (I and C) systems in today's nuclear power plants (NPPs) are based on analog technology. Thus, most existing I and C systems now face obsolescence problems. Existing NPPs have difficulty in repairing and replacing devices and boards during maintenance because manufacturers no longer produce the analog devices and boards used in the implemented I and C systems. Therefore, existing NPPs are replacing the obsolete analog I and C systems with advanced digital systems. New NPPs are also adopting digital I and C systems because the economic efficiencies and usability of the systems are higher than the analog I and C systems. Digital I and C systems are based on two technologies: a microprocessor based system in which software programs manage the required functions and a programmable logic device (PLD) based system in which programmable logic devices, such as field programmable gate arrays, manage the required functions. PLD based systems provide higher levels of performance compared with microprocessor based systems because PLD systems can process the data in parallel while microprocessor based systems process the data sequentially. In this research, a bistable trip logic in a reactor protection system (RPS) was developed using very high speed integrated circuits hardware description language (VHDL), which is a hardware description language used in electronic design to describe the behavior of the digital system. Functional verifications were also performed in order to verify that the bistable trip logic was designed correctly and satisfied the required specifications. For the functional verification, a random testing technique was adopted to generate test inputs for the bistable trip logic.

  18. Structural analysis for Diagnosis

    Izadi-Zamanabadi, Roozbeh; Blanke, M.


    Aiming at design of algorithms for fault diagnosis, structural analysis of systems offers concise yet easy overall analysis. Graph-based matching, which is the essential technique to obtain redundant information for diagnosis, is re-considered in this paper. Matching is re-formulated as a problem...

  19. Structural analysis for diagnosis

    Izadi-Zamanabadi, Roozbeh; Blanke, M.


    Aiming at design of algorithms for fault diagnosis, structural analysis of systems offers concise yet easy overall analysis. Graph-based matching, which is the essential tech-nique to obtain redundant information for diagnosis, is reconsidered in this paper. Matching is reformulated as a problem...

  20. Revised RPS Method for Complex Solution of Multimode Slab Waveguides with Metal layers

    LI Jian-jun; SHEN Guang-di


    The RPS method,which integrates the real numerical solution, the perturbation solution and the shooting solution reasonably,can obtain the complex solution of a slab waveguide with small gain/loss regions without searching the root in the entire complex plan. A revision to the RPS method is presented so that it can deal with the waveguide including large gain/loss regions. The application indicated that the simulation results are precise.

  1. Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-conditioned RPS4 auto-immunity

    Katharina eHeidrich


    Full Text Available In plant effector-triggered immunity (ETI, intracellular nucleotide binding-leucine rich repeat (NLR receptors are activated by specific pathogen effectors. The Arabidopsis TIR (Toll Interleukin1 receptor domain-NLR (denoted TNL gene pair, RPS4 and RRS1, confers resistance to Pseudomonas syringae pv tomato (Pst strain DC3000 expressing the Type III-secreted effector, AvrRps4. Nuclear accumulation of AvrRps4, RPS4 and the TNL resistance regulator EDS1 is necessary for ETI. RRS1 possesses a C-terminal ‘WRKY’ transcription factor DNA binding domain suggesting that important RPS4/RRS1 recognition and/or resistance signaling events occur at the nuclear chromatin. In Arabidopsis accession Ws-0, the RPS4Ws/RRS1Ws allelic pair governs resistance to Pst/AvrRps4 accompanied by host programmed cell death (pcd. In accession Col-0, RPS4Col/RRS1Col effectively limits Pst/AvrRps4 growth without pcd. Constitutive expression of HA-StrepII tagged RPS4Col (in a 35S:RPS4-HS line confers temperature conditioned EDS1-dependent auto-immunity. Here we show that a high (28oC, non-permissive to moderate (19oC, permissive temperature shift of 35S:RPS4-HS plants can be used to follow defense-related transcriptional dynamics without a pathogen effector trigger. By comparing responses of 35S:RPS4-HS with 35S:RPS4-HS rrs1-11 and 35S:RPS4-HS eds1-2 mutants, we establish that RPS4Col auto-immunity depends entirely on EDS1 and partially on RRS1Col. Examination of gene expression microarray data over 24h after temperature shift reveals a mainly quantitative RRS1Col contribution to up- or down-regulation of a small subset of RPS4Col-reprogrammed, EDS1-dependent genes. We find significant over-representation of WRKY transcription factor binding W-box cis-elements within the promoters of these genes. Our data show that RRS1Col contributes to temperature-conditioned RPS4Col auto-immunity and are consistent with activated RPS4Col engaging RRS1Col for resistance signaling.

  2. Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-conditioned RPS4 auto-immunity.

    Heidrich, Katharina; Tsuda, Kenichi; Blanvillain-Baufumé, Servane; Wirthmueller, Lennart; Bautor, Jaqueline; Parker, Jane E


    In plant effector-triggered immunity (ETI), intracellular nucleotide binding-leucine rich repeat (NLR) receptors are activated by specific pathogen effectors. The Arabidopsis TIR (Toll-Interleukin-1 receptor domain)-NLR (denoted TNL) gene pair, RPS4 and RRS1, confers resistance to Pseudomonas syringae pv tomato (Pst) strain DC3000 expressing the Type III-secreted effector, AvrRps4. Nuclear accumulation of AvrRps4, RPS4, and the TNL resistance regulator EDS1 is necessary for ETI. RRS1 possesses a C-terminal "WRKY" transcription factor DNA binding domain suggesting that important RPS4/RRS1 recognition and/or resistance signaling events occur at the nuclear chromatin. In Arabidopsis accession Ws-0, the RPS4(Ws) /RRS1(Ws) allelic pair governs resistance to Pst/AvrRps4 accompanied by host programed cell death (pcd). In accession Col-0, RPS4(Col) /RRS1(Col) effectively limits Pst/AvrRps4 growth without pcd. Constitutive expression of HA-StrepII tagged RPS4(Col) (in a 35S:RPS4-HS line) confers temperature-conditioned EDS1-dependent auto-immunity. Here we show that a high (28°C, non-permissive) to moderate (19°C, permissive) temperature shift of 35S:RPS4-HS plants can be used to follow defense-related transcriptional dynamics without a pathogen effector trigger. By comparing responses of 35S:RPS4-HS with 35S:RPS4-HS rrs1-11 and 35S:RPS4-HS eds1-2 mutants, we establish that RPS4(Col) auto-immunity depends entirely on EDS1 and partially on RRS1(Col) . Examination of gene expression microarray data over 24 h after temperature shift reveals a mainly quantitative RRS1(Col) contribution to up- or down-regulation of a small subset of RPS4(Col) -reprogramed, EDS1-dependent genes. We find significant over-representation of WRKY transcription factor binding W-box cis-elements within the promoters of these genes. Our data show that RRS1(Col) contributes to temperature-conditioned RPS4(Col) auto-immunity and are consistent with activated RPS4(Col) engaging RRS1(Col) for resistance

  3. Probabilistic Structural Analysis Program

    Pai, Shantaram S.; Chamis, Christos C.; Murthy, Pappu L. N.; Stefko, George L.; Riha, David S.; Thacker, Ben H.; Nagpal, Vinod K.; Mital, Subodh K.


    NASA/NESSUS 6.2c is a general-purpose, probabilistic analysis program that computes probability of failure and probabilistic sensitivity measures of engineered systems. Because NASA/NESSUS uses highly computationally efficient and accurate analysis techniques, probabilistic solutions can be obtained even for extremely large and complex models. Once the probabilistic response is quantified, the results can be used to support risk-informed decisions regarding reliability for safety-critical and one-of-a-kind systems, as well as for maintaining a level of quality while reducing manufacturing costs for larger-quantity products. NASA/NESSUS has been successfully applied to a diverse range of problems in aerospace, gas turbine engines, biomechanics, pipelines, defense, weaponry, and infrastructure. This program combines state-of-the-art probabilistic algorithms with general-purpose structural analysis and lifting methods to compute the probabilistic response and reliability of engineered structures. Uncertainties in load, material properties, geometry, boundary conditions, and initial conditions can be simulated. The structural analysis methods include non-linear finite-element methods, heat-transfer analysis, polymer/ceramic matrix composite analysis, monolithic (conventional metallic) materials life-prediction methodologies, boundary element methods, and user-written subroutines. Several probabilistic algorithms are available such as the advanced mean value method and the adaptive importance sampling method. NASA/NESSUS 6.2c is structured in a modular format with 15 elements.

  4. Arginine, leucine, and glutamine stimulate proliferation of porcine trophectoderm cells through the MTOR-RPS6K-RPS6-EIF4EBP1 signal transduction pathway.

    Kim, Jinyoung; Song, Gwonhwa; Wu, Guoyao; Gao, Haijun; Johnson, Gregory A; Bazer, Fuller W


    During the peri-implantation and early placentation periods in pigs, conceptuses (embryo and its extra-embryonic membranes) undergo dramatic morphological changes and differentiation that require the exchange of nutrients (histotroph) and gasses across the trophectoderm and a true epitheliochorial placenta. Of these nutrients, arginine (Arg), leucine (Leu), and glutamine (Gln) are essential components of histotroph; however, little is known about changes in their total amounts in the uterine lumen of cyclic and pregnant gilts and their effects on cell signaling cascades. Therefore, we determined quantities of Arg, Leu, and Gln in uterine luminal fluids and found that total recoverable amounts of these amino acids increased in pregnant but not cyclic gilts between Days 12 and 15 after onset of estrus. We hypothesized that Arg, Leu, and Gln have differential effects on hypertrophy, hyperplasia, and differentiated functions of trophectoderm cells that are critical to conceptus development. Primary porcine trophectoderm (pTr) cells treated with either Arg, Leu, or Gln had increased abundance of phosphorylated RPS6K, RPS6, and EIF4EBP1 compared to basal levels, and this effect was maintained for up to 120 min. When pTr cells were treated with Arg, Leu, and Gln, low levels of pRPS6K and pEIF4EBP1 were detected in the cytosol, but the abundance of nuclear pRPS6K increased. Immunofluorescence analyses revealed abundant amounts of pRPS6 protein in the cytoplasm of pTr cells treated with Arg, Leu, and Gln. These amino acids also increased proliferation of pTr cells. Furthermore, when Arg, Leu, and Gln were combined with siRNAs for either MTOR, RPTOR, or RICTOR, effects of those amino acids on proliferation of pTr cells were significantly inhibited. Collectively, these results indicate that Arg, Leu, and Gln act coordinately to stimulate proliferation of pTr cells through activation of the MTOR-RPS6K-RPS6-EIF4EBP1 signal transduction pathway.

  5. Reliability modeling of digital RPS with consideration of undetected software faults

    Khalaquzzaman, M.; Lee, Seung Jun; Jung, Won Dea [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Kim, Man Cheol [Chung Ang Univ., Seoul (Korea, Republic of)


    This paper provides overview of different software reliability methodologies and proposes a technic for estimating the reliability of RPS with consideration of undetected software faults. Software reliability analysis of safety critical software has been challenging despite spending a huge effort for developing large number of software reliability models, and no consensus yet to attain on an appropriate modeling methodology. However, it is realized that the combined application of BBN based SDLC fault prediction method and random black-box testing of software would provide better ground for reliability estimation of safety critical software. Digitalizing the reactor protection system of nuclear power plant has been initiated several decades ago and now full digitalization has been adopted in the new generation of NPPs around the world because digital I and C systems have many better technical features like easier configurability and maintainability over analog I and C systems. Digital I and C systems are also drift-free and incorporation of new features is much easier. Rules and regulation for safe operation of NPPs are established and has been being practiced by the operators as well as regulators of NPPs to ensure safety. The failure mechanism of hardware and analog systems well understood and the risk analysis methods for these components and systems are well established. However, digitalization of I and C system in NPP introduces some crisis and uncertainty in reliability analysis methods of the digital systems/components because software failure mechanisms are still unclear.

  6. Mutation of the cytosolic ribosomal protein-encoding RPS10B gene affects shoot meristematic function in Arabidopsis

    Stirnberg Petra


    Full Text Available Abstract Background Plant cytosolic ribosomal proteins are encoded by small gene families. Mutants affecting these genes are often viable, but show growth and developmental defects, suggesting incomplete functional redundancy within the families. Dormancy to growth transitions, such as the activation of axillary buds in the shoot, are characterised by co-ordinated upregulation of ribosomal protein genes. Results A recessive mutation in RPS10B, one of three Arabidopsis genes encoding the eukaryote-specific cytoplasmic ribosomal protein S10e, was found to suppress the excessive shoot branching mutant max2-1. rps10b-1 mildly affects the formation and separation of shoot lateral organs, including the shoot axillary meristems. Axillary meristem defects are enhanced when rps10b-1 is combined with mutations in REVOLUTA, AUXIN-RESISTANT1, PINOID or another suppressor of max2-1, FAR-RED ELONGATED HYPOCOTYL3. In some of these double mutants, the maintenance of the primary shoot meristem is also affected. In contrast, mutation of ALTERED MERISTEM PROGRAMME1 suppresses the rps10b-1axillary shoot defect. Defects in both axillary shoot formation and organ separation were enhanced by combining rps10b-1 with cuc3, a mutation affecting one of three Arabidopsis NAC transcription factor genes with partially redundant roles in these processes. To assess the effect of rps10b-1 on bud activation independently from bud formation, axillary bud outgrowth on excised cauline nodes was analysed. The outgrowth rate of untreated buds was reduced only slightly by rps10b-1 in both wild-type and max2-1 backgrounds. However, rps10b-1 strongly suppressed the auxin resistant outgrowth of max2-1 buds. A developmental phenotype of rps10b-1, reduced stamen number, was complemented by the cDNA of another family member, RPS10C, under the RPS10B promoter. Conclusions RPS10B promotes shoot branching mainly by promoting axillary shoot development. It contributes to organ boundary

  7. Structural dynamics analysis

    Housner, J. M.; Anderson, M.; Belvin, W.; Horner, G.


    Dynamic analysis of large space antenna systems must treat the deployment as well as vibration and control of the deployed antenna. Candidate computer programs for deployment dynamics, and issues and needs for future program developments are reviewed. Some results for mast and hoop deployment are also presented. Modeling of complex antenna geometry with conventional finite element methods and with repetitive exact elements is considered. Analytical comparisons with experimental results for a 15 meter hoop/column antenna revealed the importance of accurate structural properties including nonlinear joints. Slackening of cables in this antenna is also a consideration. The technology of designing actively damped structures through analytical optimization is discussed and results are presented.

  8. Nuclear accumulation of the Arabidopsis immune receptor RPS4 is necessary for triggering EDS1-dependent defense.

    Wirthmueller, Lennart; Zhang, Yan; Jones, Jonathan D G; Parker, Jane E


    Recognition of specific pathogen molecules inside the cell by nucleotide-binding domain and leucine-rich repeat (NB-LRR) receptors constitutes an important layer of innate immunity in plants. Receptor activation triggers host cellular reprogramming involving transcriptional potentiation of basal defenses and localized programmed cell death. The sites and modes of action of NB-LRR receptors are, however, poorly understood. Arabidopsis Toll/Interleukin-1 (TIR) type NB-LRR receptor RPS4 recognizes the bacterial type III effector AvrRps4. We show that epitope-tagged RPS4 expressed under its native regulatory sequences distributes between endomembranes and nuclei in healthy and AvrRps4-triggered tissues. RPS4 accumulation in the nucleus, mediated by a bipartite nuclear localization sequence (NLS) at its C terminus, is necessary for triggering immunity through authentic activation by AvrRps4 in Arabidopsis or as an effector-independent "deregulated" receptor in tobacco. A strikingly conserved feature of TIR-NB-LRR receptors is their recruitment of the nucleocytoplasmic basal-defense regulator EDS1 in resistance to diverse pathogens. We find that EDS1 is an indispensable component of RPS4 signaling and that it functions downstream of RPS4 activation but upstream of RPS4-mediated transcriptional reprogramming in the nucleus.

  9. Structural Analysis Made 'NESSUSary'


    Everywhere you look, chances are something that was designed and tested by a computer will be in plain view. Computers are now utilized to design and test just about everything imaginable, from automobiles and airplanes to bridges and boats, and elevators and escalators to streets and skyscrapers. Computer-design engineering first emerged in the 1970s, in the automobile and aerospace industries. Since computers were in their infancy, however, architects and engineers during the time were limited to producing only designs similar to hand-drafted drawings. (At the end of 1970s, a typical computer-aided design system was a 16-bit minicomputer with a price tag of $125,000.) Eventually, computers became more affordable and related software became more sophisticated, offering designers the "bells and whistles" to go beyond the limits of basic drafting and rendering, and venture into more skillful applications. One of the major advancements was the ability to test the objects being designed for the probability of failure. This advancement was especially important for the aerospace industry, where complicated and expensive structures are designed. The ability to perform reliability and risk assessment without using extensive hardware testing is critical to design and certification. In 1984, NASA initiated the Probabilistic Structural Analysis Methods (PSAM) project at Glenn Research Center to develop analysis methods and computer programs for the probabilistic structural analysis of select engine components for current Space Shuttle and future space propulsion systems. NASA envisioned that these methods and computational tools would play a critical role in establishing increased system performance and durability, and assist in structural system qualification and certification. Not only was the PSAM project beneficial to aerospace, it paved the way for a commercial risk- probability tool that is evaluating risks in diverse, down- to-Earth application

  10. Mutation of the diamond-blackfan anemia gene Rps7 in mouse results in morphological and neuroanatomical phenotypes.

    Dawn E Watkins-Chow

    Full Text Available The ribosome is an evolutionarily conserved organelle essential for cellular function. Ribosome construction requires assembly of approximately 80 different ribosomal proteins (RPs and four different species of rRNA. As RPs co-assemble into one multi-subunit complex, mutation of the genes that encode RPs might be expected to give rise to phenocopies, in which the same phenotype is associated with loss-of-function of each individual gene. However, a more complex picture is emerging in which, in addition to a group of shared phenotypes, diverse RP gene-specific phenotypes are observed. Here we report the first two mouse mutations (Rps7(Mtu and Rps7(Zma of ribosomal protein S7 (Rps7, a gene that has been implicated in Diamond-Blackfan anemia. Rps7 disruption results in decreased body size, abnormal skeletal morphology, mid-ventral white spotting, and eye malformations. These phenotypes are reported in other murine RP mutants and, as demonstrated for some other RP mutations, are ameliorated by Trp53 deficiency. Interestingly, Rps7 mutants have additional overt malformations of the developing central nervous system and deficits in working memory, phenotypes that are not reported in murine or human RP gene mutants. Conversely, Rps7 mouse mutants show no anemia or hyperpigmentation, phenotypes associated with mutation of human RPS7 and other murine RPs, respectively. We provide two novel RP mouse models and expand the repertoire of potential phenotypes that should be examined in RP mutants to further explore the concept of RP gene-specific phenotypes.

  11. Structural analysis of biodiversity.

    Sirovich, Lawrence; Stoeckle, Mark Y; Zhang, Yu


    Large, recently-available genomic databases cover a wide range of life forms, suggesting opportunity for insights into genetic structure of biodiversity. In this study we refine our recently-described technique using indicator vectors to analyze and visualize nucleotide sequences. The indicator vector approach generates correlation matrices, dubbed Klee diagrams, which represent a novel way of assembling and viewing large genomic datasets. To explore its potential utility, here we apply the improved algorithm to a collection of almost 17,000 DNA barcode sequences covering 12 widely-separated animal taxa, demonstrating that indicator vectors for classification gave correct assignment in all 11,000 test cases. Indicator vector analysis revealed discontinuities corresponding to species- and higher-level taxonomic divisions, suggesting an efficient approach to classification of organisms from poorly-studied groups. As compared to standard distance metrics, indicator vectors preserve diagnostic character probabilities, enable automated classification of test sequences, and generate high-information density single-page displays. These results support application of indicator vectors for comparative analysis of large nucleotide data sets and raise prospect of gaining insight into broad-scale patterns in the genetic structure of biodiversity.

  12. Versatile properties of an exopolysaccharide R-PS18 produced by Rhizobium sp. PRIM-18.

    Priyanka, P; Arun, A B; Ashwini, P; Rekha, P D


    Exopolysaccharides (EPS) produced by bacteria have attracted scientific and industrial attention due to their multifunctional properties and relatively easier production. In this study, an EPS viz., R-PS18 produced by Rhizobium sp. PRIM-18 was characterized and its functional properties were assessed. Cell proliferative and in vitro wound healing activities of the EPS were established using human dermal fibroblast (HDF) cells. The isolate produced 2.1 g L(-1) purified EPS (molecular weight 9.33×10(6) Da) comprising of glucose, galactose, and mannose (6.1:1.8:1). Viscosity of 0.25% solution was 23.4 mPa s (shear rate 75 s(-1)) and it showed pseudoplastic and thixotropic behavior. High emulsification, iron chelation, and superoxide scavenging abilities were also observed. Significant increase in HDF cell proliferation and wound healing in vitro was achieved by R-PS18 treatment. Sulfation of R-PS18 significantly enhanced the cell proliferative and wound healing activities. In conclusion, these findings indicate potential applications of R-PS18.

  13. Molecular characterization of rpsL gene s of streptomycin-resistant clinical isolates of mycobacteria tuberculosis in Wuhan%武汉地区结核分枝杆菌链霉素耐药分离株 rpsL 基因分子特征研究

    芦莲; 陈高瞻; 黄小琴; 雷航; 胡申才; 李睿; 刘志国; 余晓丽


    探讨武汉地区结核分枝杆菌链霉素( SM)耐药临床分离株rpsL基因分子特征。收集71株临床分离株,采用刃天青法测定临床分离株对SM的最低抑菌浓度( MIC),同时用直接测序法分析rpsL基因的突变情况;用RD105基因缺失检测法鉴定71株临床分离株中“北京基因型”菌株。结果显示20株SM敏感株rpsL基因未发现突变( MICs<0.25 mg/L);51株SM耐药株中,35株(68.6%)检测到rpsL基因突变,主要发生在第43位和88位密码子,MIC≤1 mg/L的SM耐药株(低水平耐药株)总突变率低于MIC≥2 mg/L (高水平耐药株);北京基因型与耐药株的相关性不大。研究表明SM耐药菌株rpsL基因突变类型存在地域差异;rpsL突变与SM高水平耐药的相关性在本研究中关联度不大;“北京基因型”菌株在武汉地区呈流行趋势。%To explore the molecular characteristics of the mutations in rpLs genes of streptomycin ( SM )-resistant clinical isolates of Mycobacteria tuberculosis in Wuhan .Resazurin was used to determine the SM minimum inhibito-ry concentration ( MIC) of 71 clinical isolates and direct sequencing analysis was used to detect rpsL genes, RD105 gene deletion was used to identify the Beijing genotype in the 71 clinical isolates .The results show that no mutation was found in the 20 SM susceptible isolates and the MICs of the 20 SM susceptible isolates were <0.25mg/L;of the 51 SM-resistant isolates, 35 (68.6%) carried mutations in rpsL gene ,with rpsL Lys43Arg and Lys88Arg being the most common rpsL mutations.Among SM-resistant isolates with MIC≤1 mg/L ( low-level resistant ) and MIC≥2 mg/L( high resistant ) ,3 out of 6 and 32 out of 45 isolates carried mutations .Mutations in rpsL gene of SM-resist-ant isolates varied due to geographical differences;and mutations in rpsL had no associated with SM high-level re-sistance;in addition , the Beijing genotype were prevalent in Wuhan , which

  14. Understanding modern energy policy: An evaluation of RPS mandates and behavioral nudges

    Brannan, Deborah Lynn Baker

    Climate change has emerged as one of the leading policy issues of the early 21st century. In response, a variety of policies and programs have been adopted encouraging renewable energy, energy efficiency and energy conservation. My dissertation consists of three research papers which evaluate two classes of modern energy policy in the United States: renewable energy mandates and behavioral nudges. The Renewable Portfolio Standard (RPS) is the most prominent state-level renewable energy policy in the United States and has been debated several times at the federal level. Using a fixed-effects panel data model I study the existing experience of the RPS to help inform the policy debate. In contrast with the previous literature that has predominantly studied the average effect of the RPS on renewable capacity investments I explore factors resulting in the heterogeneous effect of the RPS policy. Relying on a basic understanding the electric utility industry and the electricity dispatch process I provide insight into existing experience with the RPS. Spurred by political and economic barriers to adopting renewable energy policy, interest has increased in using motivational techniques informed by behavioral science to encourage reductions in energy consumption. Existing research has predominantly addressed residential energy consumption. The remainder of my dissertation applies well-established motivational techniques to the transportation sector. Using an experimental design, I test whether real-time feedback and social norms can encourage fuel efficient driving behavior. I find that real-time feedback has a large impact on fuel economy, particularly when aggregated across the entire vehicle fleet. I also find some evidence suggesting that social norms can encourage eco-driving, but perhaps more importantly, identify key challenges associated with using social norms in a transportation setting.

  15. MicroRNA-223-3p inhibits the angiogenesis of ischemic cardiac microvascular endothelial cells via affecting RPS6KB1/hif-1a signal pathway.

    Guo-Hua Dai

    Full Text Available BACKGROUND: MicroRNAs (miRNAs are a recently discovered class of posttranscriptional regulators of gene expression with critical functions in the angiogenesis and cardiovascular diseases; however, the details of miRNAs regulating mechanism of angiogenesis of ischemic cardiac microvascular endothelial cells (CMECs are not yet reported. METHODS AND RESULTS: This study analyzes the changes of the dynamic expression of miRNAs during the process of angiogenesis of ischemic CMECs by applying miRNA chip and real-time PCR for the first time. Compared with normal CMECs, ischemic CMECs have a specific miRNAs expression profile, in which mir-223-3p has the most significant up-regulation, especially during the process of migration and proliferation, while the up-regulation is the most significant during migration, reaching 11.02 times. Rps6kb1 is identified as a potential direct and functional target of mir-223-3p by applying bioinformatic prediction, real-time PCR and Western blot. Pathway analysis report indicates Rps6kb1 regulates the angiogenesis by participating into hif-1a signal pathway. Further analysis reveals that both the gene and protein expression of the downstream molecules VEGF, MAPK, PI3K and Akt of Rps6kb1/hif-1a signal pathway decrease significantly during the process of migration and proliferation in the ischemic CMECs. Therefore, it is confirmed that mir-223-3p inhibits the angiogenesis of CMECs, at least partly, via intervening RPS6KB1/hif-1a signal pathway and affecting the process of migration and proliferation. CONCLUSION: This study elucidates the miRNA regulating law in the angiogenesis of CMECs; mir-223-3p inhibits the process of migration and proliferation of ischemic CMECs probably via affecting RPS6KB1/hif-1a signal pathway, which in turn suppresses the angiogenesis. It is highly possible that mir-223-3p becomes a novel intervention core target in the treatment of angiogenesis of ischemic heart diseases.

  16. Phylogeny of Ptychostomum (Bryaceae, Musci) inferred from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) and chloroplast rps4

    Chen-Ying WANG; Jian-Cheng ZHAO


    The phylogeny of Ptychostomum was first undertaken based on analysis of the internal transcribed spacer (ITS) region of the nuclear ribosomal (nr) DNA and by combining data from nrDNA ITS and chloroplast DNA rps4 sequences. Maximum parsimony, maximum likelihood, and Bayesian analyses all support the conclu-sion that the reinstated genus Ptychostomum is not monophyletic. Ptychostomum funkii (Schwagr.) J. R. Spence (= Bryum funkii Schwagr.) is placed within a clade containing the type species of Bryum, B. argenteum Hedw. The remaining members of Ptychostomum investigated in the present study constitute another well-supported clade. The results are congruent with previous molecular analyses. On the basis of phylogenetic evidence, we agree with Bryum lonchocaulon Mull. Hal., Bryum pallescens Schleich. ex Schwagr., and Bryum pallens Sw. to Ptychostomum.

  17. TNB Experience in Developing Solar Hybrid Station at RPS Kemar, Gerik, Perak Darul Ridzuan

    Aziz, K. A.; Shamsudin, K. N.


    This paper will discuss on TNB experience in developing Solar Hybrid Station at RPS Kemar, Gerik, Perak. TNB has been approached by KKLW to submit proposal to provide electricity in the rural area namely RPS Kemar. Looking at area and source available, Solar Hybrid System was the best method in order to provide electricity at this area. This area is far from national grid sources. Solar Hybrid System is the best method to produce electrical power using the renewable energy from Solar PV, Battery and Diesel Generator Set. Nowadays, price of petroleum is slightly high due to higher demand from industry. Solar energy is good alternative in this country to practice in order to reduce cost for produce of electrical energy. Generally, Solar will produce energy during daytime and when become cloudy and dark, automatically battery and diesel generator set will recover the system through the hybrid controller system.

  18. The role of autophagy in chloroplast degradation and chlorophagy in immune defenses during Pst DC3000 (AvrRps4 infection.

    Junjian Dong

    Full Text Available BACKGROUND: Chlorosis of leaf tissue normally observed during pathogen infection may result from the degradation of chloroplasts. There is a growing evidence to suggest that the chloroplast plays a significant role during pathogen infection. Although most degradation of the organelles and cellular structures in plants is mediated by autophagy, its role in chloroplast catabolism during pathogen infection is largely unknown. RESULTS: In this study, we investigated the function of autophagy in chloroplast degradation during avirulent Pst DC3000 (AvrRps4 infection. We examined the expression of defensive marker genes and suppression of bacterial growth using the electrolyte leakage assay in normal light (N and low light (L growing environments of wild-type and atg5-1 plants during pathogen treatment. Stroma-targeted GFP proteins (CT-GFP were observed with LysoTracker Red (LTR staining of autophagosome-like structures in the vacuole. The results showed that Arabidopsis expressed a significant number of small GFP-labeled bodies when infected with avirulent Pst DC3000 (AvrRps4. While barely detectable, there were small GFP-labeled bodies in plants with the CT-GFP expressing atg5-1 mutation. The results showed that chloroplast degradation depends on autophagy and this may play an important role in inhibiting pathogen growth. CONCLUSION: Autophagy plays a role in chloroplast degradation in Arabidopsis during avirulent Pst DC3000 (AvrRps4 infection. Autophagy dependent chloroplast degradation may be the primary source of reactive oxygen species (ROS as well as the pathogen-response signaling molecules that induce the defense response.

  19. Structures and their analysis

    Fuchs, Maurice Bernard


    Addressing structures, this book presents a classic discipline in a modern setting by combining illustrated examples with insights into the solutions. It is the fruit of the author’s many years of teaching the subject and of just as many years of research into the design of optimal structures. Although intended for an advanced level of instruction it has an undergraduate course at its core. Further, the book was written with the advantage of having massive computer power in the background, an aspect which changes the entire approach to many engineering disciplines and in particular to structures. This paradigm shift has dislodged the force (flexibility) method from its former prominence and paved the way for the displacement (stiffness) method, despite the multitude of linear equations it spawns. In this book, however, both methods are taught: the force method offers a perfect vehicle for understanding structural behavior, bearing in mind that it is the displacement method which does the heavy number crunch...

  20. Structured Analysis - IDEF0

    Larsen, Michael Holm


    that require a modelling technique for the analysis, development, re-engineering, integration, or acquisition of information systems; and incorporate a systems or enterprise modelling technique into a business process analysis or software engineering methodology.This note is a summary of the Standard...

  1. An evaluation of the impact of state Renewable Portfolio Standards (RPS) on retail, commercial, and industrial electricity prices

    Puram, Rakesh

    The Renewable Portfolio Standard (RPS) has become a popular mechanism for states to promote renewable energy and its popularity has spurred a potential bill within Congress for a nationwide Federal RPS. While RPS benefits have been touted by several groups, it also has detractors. Among the concerns is that RPS standards could raise electricity rates, given that renewable energy is costlier than traditional fossil fuels. The evidence on the impact of RPS on electricity prices is murky at best: Complex models by NREL and USEIA utilize computer programs with several assumptions which make empirical studies difficult and only predict slight increases in electricity rates associated with RPS standards. Recent theoretical models and empirical studies have found price increases, but often fail to comprehensively include several sets of variables, which in fact could confound results. Utilizing a combination of past papers and studies to triangulate variables this study aims to develop both a rigorous fixed effects regression model as well as a theoretical framework to explain the results. This study analyzes state level panel data from 2002 to 2008 to analyze the effect of RPS on residential, commercial, and industrial electricity prices, controlling for several factors including amount of electricity generation from renewable and non-renewable sources, customer incentives for renewable energy, macroeconomic and demographic indicators, and fuel price mix. The study contrasts several regressions to illustrate important relationships and how inclusions as well as exclusion of various variables have an effect on electricity rates. Regression results indicate that the presence of RPS within a state increases the commercial and residential electricity rates, but have no discernable effect on the industrial electricity rate. Although RPS tends to increase electricity prices, the effect has a small impact on higher electricity prices. The models also indicate that jointly all

  2. A child with mild X-linked intellectual disability and a microduplication at Xp22.12 including RPS6KA3.

    Tejada, María-Isabel; Martínez-Bouzas, Cristina; García-Ribes, Ainhoa; Larrucea, Susana; Acquadro, Francesco; Cigudosa, Juan-C; Belet, Stefanie; Froyen, Guy; López-Aríztegui, Maria-Asun


    Multiplex ligation-dependent probe amplification (MLPA) and array- comparative genomic hybridization analysis have been proven to be useful in the identification of submicroscopic copy-number imbalances in families with nonsyndromic X-linked intellectual disability (NS-XLID). Here we report the first description of a child with mild intellectual disability and a submicroscopic duplication at Xp22.12 identified by MLPA with a P106 MRX kit (MRC-Holland, Amsterdam, Netherlands) and further confirmed and characterized with a custom 244-k oligo-array, fluorescence in situ hybridization, quantitative polymerase chain reaction (qPCR), and immunoblotting. This 1.05-megabase duplication encompasses 7 genes, RPS6KA3 being the only of these genes known to be related to ID. The proband was an 8-year-old boy referred to the genetics unit for psychomotor retardation and learning disabilities. Both maternal brothers also showed learning difficulties and delayed language during childhood in a similar way to the proband. These boys also carried the duplication, as did the healthy mother and grandmother of the proband. The same duplication was also observed in the 5-year-old younger brother who presented with features of developmental delay and learning disabilities during the previous year. Increased RPS6KA3/RSK2 levels were demonstrated in the proband by qPCR and immunoblotting. To our knowledge, this is the first family identified with a submicroscopic duplication including the entire RPS6KA3/RSK2 gene, and our findings suggest that an increased dose of this gene is responsible for a mild form of NS-XLID.

  3. Collapse Analysis of Timber Structures

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard


    A probabilistic based collapse analysis has been performed for a glulam frame structure supporting the roof over the main court in a Norwegian sports centre. The robustness analysis is based on the framework for robustness analysis introduced in the Danish Code of Practice for the Safety...... of Structures and a probabilistic modelling of the timber material proposed in the Probabilistic Model Code (PMC) of the Joint Committee on Structural Safety (JCSS). Due to the framework in the Danish Code the timber structure has to be evaluated with respect to the following criteria where at least one shall...... be fulfilled: a) demonstrating that those parts of the structure essential for the safety only have little sensitivity with respect to unintentional loads and defects, or b) demonstrating a load case with „removal of a limited part of the structure‟ in order to document that an extensive failure...

  4. Thai Rhetorical Structure Analysis

    Sinthupoun, Somnuk


    A rhetorical structure tree (RS tree) is a representation of discourse relations among elementary discourse units (EDUs). A RS tree is very useful to many text processing tasks employing relationships among EDUs such as text understanding, summarization, and question answering. Thai language with its unique linguistic characteristics requires a unique RS tree construction technique. This paper proposes an approach for Thai RS tree construction which consists of three major steps: EDU segmentation, Thai RS tree construction, and discourse relation (DR) identification. Two hidden markov models derived from grammatical rules are used to segment EDUs, a clustering technique with its similarity measure derived from Thai semantic rules is used to construct a Thai RS tree, and a decision tree whose features extracted from the rules is used to determine the DR between EDUs. The proposed technique is evaluated using three Thai corpora. The results show the Thai RS tree construction and the DR identification effectiven...

  5. Regularized Generalized Structured Component Analysis

    Hwang, Heungsun


    Generalized structured component analysis (GSCA) has been proposed as a component-based approach to structural equation modeling. In practice, GSCA may suffer from multi-collinearity, i.e., high correlations among exogenous variables. GSCA has yet no remedy for this problem. Thus, a regularized extension of GSCA is proposed that integrates a ridge…

  6. Synthetic Lethality Screen Identifies RPS6KA2 as Modifier of Epidermal Growth Factor Receptor Activity in Pancreatic Cancer

    Nada Milosevic


    Full Text Available Pancreatic cancer is characterized by a high degree of resistance to chemotherapy. Epidermal growth factor receptor (EGFR inhibition using the small-molecule inhibitor erlotinib was shown to provide a small survival benefit in a subgroup of patients. To identify kinases whose inhibition acts synergistically with erlotinib, we employed a kinome-wide small-interfering RNA (siRNA-based loss-of-function screen in the presence of erlotinib. Of 779 tested kinases, we identified several targets whose inhibition acted synergistically lethal with EGFR inhibition by erlotinib, among them the S6 kinase ribosomal protein S6 kinase 2 (RPS6KA2/ribosomal S6 kinase 3. Activated RPS6KA2 was expressed in approximately 40% of 123 human pancreatic cancer tissues. RPS6KA2 was shown to act downstream of EGFR/RAS/mitogen-activated protein kinase kinase (MEK/extracellular-signal regulated kinase (ERK signaling and was activated by EGF independently of the presence of KRAS mutations. Knockdown of RPS6KA2 by siRNA led to increased apoptosis only in the presence of erlotinib, whereas RPS6KA2 activation or overexpression rescued from erlotinib- and gemcitabine-induced apoptosis. This effect was at least in part mediated by downstream activation of ribosomal protein S6. Genetic as well as pharmacological inhibition of RPS6KA2 by the inhibitor BI-D1870 acted synergistically with erlotinib. By applying this synergistic lethality screen using a kinome-wide RNA interference-library approach, we identified RPS6KA2 as potential drug target whose inhibition synergistically enhanced the effect of erlotinib on tumor cell survival. This kinase therefore represents a promising drug candidate suitable for the development of novel inhibitors for pancreatic cancer therapy.

  7. Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer

    Lorenzo-Diaz, Fabian; Fernández-Lopez, Cris; Douarre, Pierre-Emmanuel; Baez-Ortega, Adrian; Flores, Carlos; Glaser, Philippe


    Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 3′-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal

  8. Evolutionary aspects of a unique internal mitochondrial targeting signal in nuclear-migrated rps19 of sugar beet (Beta vulgaris L.).

    Matsunaga, Muneyuki; Takahashi, Yoshiya; Yui-Kurino, Rika; Mikami, Tetsuo; Kubo, Tomohiko


    The endosymbiotic theory postulates that many genes migrated from endosymbionts to the nuclear genomes of their hosts. Some migrated genes lack presequences directing proteins to mitochondria, and their mitochondrial targeting signals appear to be inscribed in the core coding regions as internal targeting signals (ITSs). ITSs may have evolved after sequence transfer to nuclei or ITSs may have pre-existed before sequence transfer. Here, we report the molecular cloning of a sugar beet gene for ribosomal protein S19 (Rps19; the first letter is capitalized when the gene is a nuclear gene). We show that sugar beet Rps19 (BvRps19) is an ITS-type gene. Based on amino-acid sequence comparison, dicotyledonous rps19s (the first letter is lower-cased when the gene is a mitochondrial gene), such as tobacco rps19 (Ntrps19), resemble an ancestral form of BvRps19. We investigated whether differences in amino-acid sequences between BvRps19 and Ntrps19 were involved in ITS evolution. Analyses of the intracellular localization of chimaeric GFP-fusion proteins that were transiently expressed in Welsh onion cells showed that Ntrps19-gfp was not localized in mitochondria. When several BvRps19-type amino acid substitutions, none of which was seen in any other angiosperm rps19, were introduced into Ntrps19-gfp, the modified Ntrps19-gfp became localized in mitochondria, supporting the notion that an ITS in BvRps19 evolved following sequence transfer to nuclei. Not all of these substitutions were seen in other ITS-type Rps19s, suggesting that the ITSs of Rps19 are diverse.

  9. Characterisation of rpsL, rrs and embB mutations associated with streptomycin and ethambutol resistance in Mycobacterium tuberculosis.

    Tracevska, Tatjana; Jansone, Inta; Nodieva, Anda; Marga, Olgerts; Skenders, Girts; Baumanis, Viesturs


    In order to characterise molecular mechanisms of first-line drug resistance in Mycobacterium tuberculosis and to evaluate the use of molecular markers of resistance (gene point mutations), we analysed 66 multi-drug-resistant (MDR) isolates from Latvian tuberculosis patients. They were all resistant to rifampin (RIF), isoniazid (INH) and streptomycin (SM), and 33 were resistant to ethambutol (EMB). Enzymatic digestion by MboII and nucleotide sequencing of the rpsL gene fragment detected a single nucleotide substitution K43R in 40 (61%) of the 66 SM-resistant M. tuberculosis isolates. Of the other 26 SM-resistant isolates, 16 (24%) had mutations at positions 513A-->C and 516C-->T of the rrs gene and 10 (15%) had the wild-type sequence. The single-stranded DNA conformation polymorphism (SSCP) method was used to detect mutations in the embB gene associated with EMB resistance. Substitutions in the embB gene were found by SSCP analysis in 15 (45%) and by sequencing in 17 (52%) of the 33 EMB-resistant isolates. Surprisingly, SSCP revealed a nucleotide mutation at codon M306 in five (15%) of 33 in vitro EMB-susceptible MDR isolates.

  10. Structural Analysis of Communication Development.

    Conville, Richard L.

    This paper discusses the question of the legitimacy of applying structural analysis to actual human behavior and illustrates its legitimacy by using the reasoning in an essay by Paul Ricoeur. It then asks if the principles of communication development (obliqueness, exchange, and dying) derived from Helen Keller's experience of communication…

  11. Structural Analysis of Plate Based Tensegrity Structures

    Hald, Frederik; Kirkegaard, Poul Henning; Damkilde, Lars


    Plate tensegrity structures combine tension cables with a cross laminated timber plate and can then form e.g. a roof structure. The topology of plate tensegrity structures is investigated through a parametric investigation. Plate tensegrity structures are investigated, and a method...... for determination of the structures pre-stresses is used. A parametric investigation is performed to determine a more optimized form of the plate based tensegrity structure. Conclusions of the use of plate based tensegrity in civil engineering and further research areas are discussed....

  12. Structural Analysis of Plate Based Tensegrity Structures

    Hald, Frederik; Kirkegaard, Poul Henning; Damkilde, Lars


    Plate tensegrity structures combine tension cables with a cross laminated timber plate and can then form e.g. a roof structure. The topology of plate tensegrity structures is investigated through a parametric investigation. Plate tensegrity structures are investigated, and a method...... for determination of the structures pre-stresses is used. A parametric investigation is performed to determine a more optimized form of the plate based tensegrity structure. Conclusions of the use of plate based tensegrity in civil engineering and further research areas are discussed....

  13. Efficient Analysis of Complex Structures

    Kapania, Rakesh K.


    Last various accomplishments achieved during this project are : (1) A Survey of Neural Network (NN) applications using MATLAB NN Toolbox on structural engineering especially on equivalent continuum models (Appendix A). (2) Application of NN and GAs to simulate and synthesize substructures: 1-D and 2-D beam problems (Appendix B). (3) Development of an equivalent plate-model analysis method (EPA) for static and vibration analysis of general trapezoidal built-up wing structures composed of skins, spars and ribs. Calculation of all sorts of test cases and comparison with measurements or FEA results. (Appendix C). (4) Basic work on using second order sensitivities on simulating wing modal response, discussion of sensitivity evaluation approaches, and some results (Appendix D). (5) Establishing a general methodology of simulating the modal responses by direct application of NN and by sensitivity techniques, in a design space composed of a number of design points. Comparison is made through examples using these two methods (Appendix E). (6) Establishing a general methodology of efficient analysis of complex wing structures by indirect application of NN: the NN-aided Equivalent Plate Analysis. Training of the Neural Networks for this purpose in several cases of design spaces, which can be applicable for actual design of complex wings (Appendix F).

  14. Marine Structural Analysis and Design


    王迎光编上海交通大学出版社出版定价:$68.00内容简介:The material in this book has been continuously developed since the author started to teach Modern Ship Structural Design in the Department of Naval Architecture and Ocean Engineering of Shanghai Jiao Tong University in 2004.The subject of marine structural analysis and design is so broad that it is not possible to incorporate every aspect of this sub-

  15. Structural Analysis of Complex Networks

    Dehmer, Matthias


    Filling a gap in literature, this self-contained book presents theoretical and application-oriented results that allow for a structural exploration of complex networks. The work focuses not only on classical graph-theoretic methods, but also demonstrates the usefulness of structural graph theory as a tool for solving interdisciplinary problems. Applications to biology, chemistry, linguistics, and data analysis are emphasized. The book is suitable for a broad, interdisciplinary readership of researchers, practitioners, and graduate students in discrete mathematics, statistics, computer science,

  16. Structured Functional Principal Component Analysis

    Shou, Haochang; Zipunnikov, Vadim; Crainiceanu, Ciprian M.; Greven, Sonja


    Summary Motivated by modern observational studies, we introduce a class of functional models that expand nested and crossed designs. These models account for the natural inheritance of the correlation structures from sampling designs in studies where the fundamental unit is a function or image. Inference is based on functional quadratics and their relationship with the underlying covariance structure of the latent processes. A computationally fast and scalable estimation procedure is developed for high-dimensional data. Methods are used in applications including high-frequency accelerometer data for daily activity, pitch linguistic data for phonetic analysis, and EEG data for studying electrical brain activity during sleep. PMID:25327216

  17. Synthesis of a spatial 3-RPS parallel manipulator based on physical constraints

    Nalluri Mohan Rao


    The range of motion of the moving platform of a spatial 3-RPS parallel manipulator will be greatly influenced by the physical constraints such as limits on the lengths of the limbs and the range of motion of the spherical joints. Therefore, while synthesizing the parallel manipulator, the physical constraints have to be considered. Synthesis of the manipulator involves determination of the architectural parameters of the manipulator so that a point on the moving platform passes through a prescribed set of positions in space. This paper presents a synthesis procedure that determines location and direction of revolute joints and location of spherical joints along with orientation of sockets of spherical joints, considering the physical constraints. The synthesis procedure is demonstrated through a numerical example.

  18. Implementation of a RPS Cyber Security Test-bed with Two PLCs

    Shin, Jinsoo; Heo, Gyunyoung [Kyung Hee Univ., Yongin (Korea, Republic of); Son, Hanseong [Joongbu Univ., Geumsan (Korea, Republic of); An, Yongkyu; Rizwan, Uddin [University of Illinois at Urbana-Champaign, Urbana (United States)


    Our research team proposed the methodology to evaluate cyber security with Bayesian network (BN) as a cyber security evaluation model and help operator, licensee, licensor or regulator in granting evaluation priorities. The methodology allowed for overall evaluation of cyber security by considering architectural aspect of facility and management aspect of cyber security at the same time. In order to emphasize reality of this model by inserting true data, it is necessary to conduct a penetration test that pretends an actual cyber-attack. Through the collaboration with University of Illinois at Urbana-Champaign, which possesses the Tricon a safety programmable logic controller (PLC) used at nuclear power plants and develops a test-bed for nuclear power plant, a test-bed for reactor protection system (RPS) is being developed with the PLCs. Two PLCs are used to construct a simple test-bed for RPS, bi-stable processor (BP) and coincidence processor (CP). By using two PLCs, it is possible to examine cyber-attack against devices such as PLC, cyber-attack against communication between devices, and the effects of a PLC on the other PLC. Two PLCs were used to construct a test-bed for penetration test in this study. Advantages of using two or more PLCs instead of single PLC are as follows. 1) Results of cyber-attack reflecting characteristics among PLCs can be obtained. 2) Cyber-attack can be attempted using a method of attacking communication between PLCs. True data obtained can be applied to existing cyber security evaluation model to emphasize reality of the model.

  19. RPS6KA2, a putative tumour suppressor gene at 6q27 in sporadic epithelial ovarian cancer

    Bignone, P A; Lee, K Y; Liu, Y


    -activated protein kinase pathway. It is expressed in normal ovarian epithelium, whereas reduced or absent in tumours or cell lines. We show that RPS6KA2 is monoallelically expressed in the ovary suggesting that loss of a single expressed allele is sufficient to cause complete loss of expression in cancer cells...

  20. Mammalian ribosomal and chaperone protein RPS3A counteracts α-synuclein aggregation and toxicity in a yeast model system.

    De Graeve, Stijn; Marinelli, Sarah; Stolz, Frank; Hendrix, Jelle; Vandamme, Jurgen; Engelborghs, Yves; Van Dijck, Patrick; Thevelein, Johan M


    Accumulation of aggregated forms of αSyn (α-synuclein) into Lewy bodies is a known hallmark associated with neuronal cell death in Parkinson's disease. When expressed in the yeast Saccharomyces cerevisiae, αSyn interacts with the plasma membrane, forms inclusions and causes a concentration-dependent growth defect. We have used a yeast mutant, cog6Δ, which is particularly sensitive to moderate αSyn expression, for screening a mouse brain-specific cDNA library in order to identify mammalian proteins that counteract αSyn toxicity. The mouse ribosomal and chaperone protein RPS3A was identified as a suppressor of αSyn [WT (wild-type) and A53T] toxicity in yeast. We demonstrated that the 50 N-terminal amino acids are essential for this function. The yeast homologues of RPS3A were not effective in suppressing the αSyn-induced growth defect, illustrating the potential of our screening system to identify modifiers that would be missed using yeast gene overexpression as the first screening step. Co-expression of mouse RPS3A delayed the formation of αSyn-GFP inclusions in the yeast cells. The results of the present study suggest that the recently identified extraribosomal chaperonin function of RPS3A also acts on the neurodegeneration-related protein αSyn and reveal a new avenue for identifying promising candidate mammalian proteins involved in αSyn functioning.

  1. Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8

    De Bortoli Massimiliano


    Full Text Available Abstract Background Medulloblastoma is the most common malignant brain tumor of childhood. Improvements in clinical outcome require a better understanding of the genetic alterations to identify clinically significant biological factors and to stratify patients accordingly. In the present study, we applied cytogenetic characterization to guide the identification of biologically significant genes from gene expression microarray profiles of medulloblastoma. Methods We analyzed 71 primary medulloblastomas for chromosomal copy number aberrations (CNAs using comparative genomic hybridization (CGH. Among 64 tumors that we previously analyzed by gene expression microarrays, 27 were included in our CGH series. We analyzed clinical outcome with respect to CNAs and microarray results. We filtered microarray data using specific CNAs to detect differentially expressed candidate genes associated with survival. Results The most frequent lesions detected in our series involved chromosome 17; loss of 16q, 10q, or 8p; and gain of 7q or 2p. Recurrent amplifications at 2p23-p24, 2q14, 7q34, and 12p13 were also observed. Gain of 8q is associated with worse overall survival (p = 0.0141, which is not entirely attributable to MYC amplification or overexpression. By applying CGH results to gene expression analysis of medulloblastoma, we identified three 8q-mapped genes that are associated with overall survival in the larger group of 64 patients (p EEF1D, ribosomal protein L30 (RPL30, and ribosomal protein S20 (RPS20. Conclusion The complementary use of CGH and expression profiles can facilitate the identification of clinically significant candidate genes involved in medulloblastoma growth. We demonstrate that gain of 8q and expression levels of three 8q-mapped candidate genes (EEF1D, RPL30, RPS20 are associated with adverse outcome in medulloblastoma.

  2. Soil Retaining Structures: Development of models for structural analysis

    Bakker, K.J.


    The topic of this thesis is the development of models for the structural analysis of soil retaining structures. The soil retaining structures being looked at are; block revetments, flexible retaining walls and bored tunnels in soft soil. Within this context typical structural behavior of these struc

  3. Venus Mobile Explorer with RPS for Active Cooling: A Feasibility Study

    Leifer, Stephanie D.; Green, Jacklyn R.; Balint, Tibor S.; Manvi, Ram


    We present our findings from a study to evaluate the feasibility of a radioisotope power system (RPS) combined with active cooling to enable a long-duration Venus surface mission. On-board power with active cooling technology featured prominently in both the National Research Council's Decadal Survey and in the 2006 NASA Solar System Exploration Roadmap as mission-enabling for the exploration of Venus. Power and cooling system options were reviewed and the most promising concepts modeled to develop an assessment tool for Venus mission planners considering a variety of future potential missions to Venus, including a Venus Mobile Explorer (either a balloon or rover concept), a long-lived Venus static lander, or a Venus Geophysical Network. The concepts modeled were based on the integration of General Purpose Heat Source (GPHS) modules with different types of Stirling cycle heat engines for power and cooling. Unlike prior investigations which reported on single point design concepts, this assessment tool allows the user to generate either a point design or parametric curves of approximate power and cooling system mass, power level, and number of GPHS modules needed for a "black box" payload housed in a spherical pressure vessel.

  4. Analysis of Pumphouse RCC Frame Structure for Soil Structure Interaction

    Mr A.S. Thombare


    Full Text Available When structure is built on ground some elements of structure are direct contact with soil. When loads are applied on structure internal forces are developed in both the structure as well as in soil. It results in deformation of both the components which are independent to each other. This are called soil structure interaction. The analysis is done by using (Bentley STAAD.Pro V8i Version 2007 software. The analysis carried out been pump house structure R.C.C. frame structure find out shear force Z direction fixed support and fixed but support for different soil. It concluded that soil structure interaction more affected on fixed base. So overcome the effects of the soil structure interaction on the soft soil, it is important to design the structure to standard loading condition and interaction forces. Thus here concluded that pump house building should be design resist the maximum shear force in fixed base

  5. Robustness Analysis of Kinetic Structures

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard


    The present paper considers robustness of kinetic structures. Robustness of structures has obtained a renewed interest due to a much more frequent use of advanced types of structures with limited redundancy and serious consequences in case of failure. Especially for these types of structural syst...... systems, it is of interest to investigate how robust the structures are, or what happens if a structural element is added to or removed from the original structure. The present paper discusses this issue for kinetic structures in architecture....

  6. Comparative sequence analysis of the complete set of 40S ribosomal proteins in the Senegalese sole (Solea senegalensis Kaup and Atlantic halibut (Hippoglossus hippoglossus L. (Teleostei: Pleuronectiformes: phylogeny and tissue- and development-specific expression

    Cañavate Jose


    Full Text Available Abstract Background Ribosomal proteins (RPs are key components of ribosomes, the cellular organelle responsible for protein biosynthesis in cells. Their levels can vary as a function of organism growth and development; however, some RPs have been associated with other cellular processes or extraribosomal functions. Their high representation in cDNA libraries has resulted in the increase of RP sequences available from different organisms and their proposal as appropriate molecular markers for phylogenetic analysis. Results The development of large-scale genomics of Senegalese sole (Solea senegalensis and Atlantic halibut (Hippoglossus hippoglossus, two commercially important flatfish species, has made possible the identification and systematic analysis of the complete set of RP sequences for the small (40S ribosome subunit. Amino acid sequence comparisons showed a high similarity both between these two flatfish species and with respect to other fish and human. EST analysis revealed the existence of two and four RPS27 genes in Senegalese sole and Atlantic halibut, respectively. Phylogenetic analysis clustered RPS27 in two separate clades with their fish and mammalian counterparts. Steady-state transcript levels for eight RPs (RPS2, RPS3a, RPS15, RPS27-1, RPS27-2, RPS27a, RPS28, and RPS29 in sole were quantitated during larval development and in tissues, using a real-time PCR approach. All eight RPs exhibited different expression patterns in tissues with the lowest levels in brain. On the contrary, RP transcripts increased co-ordinately after first larval feeding reducing progressively during the metamorphic process. Conclusion The genomic resources and knowledge developed in this survey will provide new insights into the evolution of Pleuronectiformes. Expression data will contribute to a better understanding of RP functions in fish, especially the mechanisms that govern growth and development in larvae, with implications in aquaculture.

  7. Design and analysis of heliostat support structure

    Zang Chuncheng; Wang Zhifeng [Inst. of Electrical Engineering, CAS, BJ (China); Liu Xiaobing; Zhang Xiliang [Himin Solar Energy Group Co. Ltd, Dezhou, SD (China); Wang Yanzhong [Beijing Univ. of Aeronautics and Astronautics, BJ (China)


    The design method of the heliostat support structure with the aim of reducing the cost maximally is described in this paper. In order to guarantee the strength, stiffness and stability of the structure, dynamic performance and static performance including internal stress and distortion are analyzed by means of VSAP (Visual Structural Analysis Program) finite element computational software. Then the support structure is optimized on the basis of the analysis. (orig.)

  8. Convergent Evolution of Fern-Specific Mitochondrial Group II Intron atp1i361g2 and Its Ancient Source Paralogue rps3i249g2 and Independent Losses of Intron and RNA Editing among Pteridaceae

    Zumkeller, Simon Maria; Knoop, Volker; Knie, Nils


    Mitochondrial intron patterns are highly divergent between the major land plant clades. An intron in the atp1 gene, atp1i361g2, is an example for a group II intron specific to monilophytes (ferns). Here, we report that atp1i361g2 is lost independently at least 4 times in the fern family Pteridaceae. Such plant organelle intron losses have previously been found to be accompanied by loss of RNA editing sites in the flanking exon regions as a consequence of genomic recombination of mature cDNA. Instead, we now observe that RNA editing events in both directions of pyrimidine exchange (C-to-U and U-to-C) are retained in atp1 exons after loss of the intron in Pteris argyraea/biaurita and in Actiniopteris and Onychium. We find that atp1i361g2 has significant similarity with intron rps3i249g2 present in lycophytes and gymnosperms, which we now also find highly conserved in ferns. We conclude that atp1i361g2 may have originated from the more ancestral rps3i249g2 paralogue by a reverse splicing copy event early in the evolution of monilophytes. Secondary structure elements of the two introns, most characteristically their domains III, show strikingly convergent evolution in the monilophytes. Moreover, the intron paralogue rps3i249g2 reveals relaxed evolution in taxa where the atp1i361g2 paralogue is lost. Our findings may reflect convergent evolution of the two related mitochondrial introns exerted by co-evolution with an intron-binding protein simultaneously acting on the two paralogues. PMID:27492234

  9. Structural analysis considerations for wind turbine blades

    Spera, D. A.


    Approaches to the structural analysis of wind turbine blade designs are reviewed. Specifications and materials data are discussed along with the analysis of vibrations, loads, stresses, and failure modes.

  10. Analysis of piezoelectric structures and devices

    Chen, Weiqiu; Wang, Ji


    This edited work covers piezoelectric materials in the form of beams, plates, shells, and other structural components in modern devices and structures. Applications are frequency control and detection functions in resonators, sensors, actuators, oscillations, and other smart and intelligent structures. The contributions cover novel methods for the analysis of piezoelectric structures including wave propagation, high frequency vibration, material characterization, and optimization of structures. Understanding of these methods is increasingly important in the design and modelling of next generat

  11. Robustness Analysis of Kinetic Structures

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard


    Kinetic structures in architecture follows a new trend which is emerging in responsive architecture coined by Nicholas Negroponte when he proposed that architecture may benefit from the integration of computing power into built spaces and structures, and that better performing, more rational...

  12. Robustness Analysis of Kinetic Structures

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard


    Kinetic structures in architecture follows a new trend which is emerging in responsive architecture coined by Nicholas Negroponte when he proposed that architecture may benefit from the integration of computing power into built spaces and structures, and that better performing, more rational...


    Josip Peko


    Full Text Available This study examined steel and aluminum variants of modern exhibition structures in which the main design requirements include low weight (increased span/depth ratio, transportation, and construction and durability (resistance to corrosion. This included a design situation in which the structural application of aluminum alloys provided an extremely convenient and practical solution. Viability of an aluminum structure depends on several factors and requires a detailed analysis. The overall conclusion of the study indicated that aluminum can be used as a structural material and as a viable alternative to steel for Croatian snow and wind load values and evidently in cases in which positive properties of aluminum are required for structural design. Furthermore, a structural fire analysis was conducted for an aluminum variant structure by using a zone model for realistic fire analysis. The results suggested that passive fire protection for the main structural members was not required in the event of areal fire with duration of 60 min.

  14. Stiffness Improvement of 3RPS PKM by Redundant Actuating Leg%冗余驱动支链对3RPS 并联机构刚度的改善

    崔学良; 陈五一; 韩先国; 祝锡晶; 王建青; 成全


    在考虑雅可比矩阵变化与动平台姿态偏移之间映射关系的基础上,基于螺旋理论和矢量微分法建立了3RPS 并联机构末端位姿偏移与支链构件变形之间的映射模型。首先采用螺旋理论和矢量微分法分析了支链各构件刚度与整个支链刚度之间的关系,然后建立了3RPS 并联机构的瞬时刚度模型,并分析了雅可比矩阵的变化对机构刚度的影响。对冗余驱动支链改善并联机构刚度的原理进行了分析,建立了冗余驱动并联机构的整机刚度模型。仿真分析和实验验证,冗余驱动支链确实能够改善并联机构的刚度。%Considering the relationship between the changes of the Jacobian matrix and the orien-tation deviation of the moving platform,the mapping model between the 3RPS PKM end-effector off-set and the deformation of the branches was established by using the vector differential method,based on the screw theory.The functional expression of the rigidity of the chain and its components was de-rived,and then the instant stiffness model of the 3RPS PKM was built up.The influences of the chan-ges of the Jacobian matrix on the stiffness of the PKM were analyzed.The theories that the redundant actuating leg could increase the stiffness of the PKM were discussed,and were also verified by the simulation and the tests.

  15. NASA Structural Analysis System (NASTRAN)

    Purves, L.


    Program aids in structural design of wide range of objects, from high-impact printer parts to turbine engine blades, and fully validated. Since source code included, NASTRAN modified or enhanced for new applications.

  16. The Intelligent Use of Structural Analysis.

    Grierson, Donald E.


    Discusses the use of sensitivity analysis in structural design computation. Such analysis can predict how response quantities will vary with given design changes. In addition, it can help student engineers better understand why and when particular structural analyses are or should be conducted. (JN)

  17. Nonlinear structural analysis using integrated force method

    N R B Krishnam Raju; J Nagabhushanam


    Though the use of the integrated force method for linear investigations is well-recognised, no efforts were made to extend this method to nonlinear structural analysis. This paper presents the attempts to use this method for analysing nonlinear structures. General formulation of nonlinear structural analysis is given. Typically highly nonlinear bench-mark problems are considered. The characteristic matrices of the elements used in these problems are developed and later these structures are analysed. The results of the analysis are compared with the results of the displacement method. It has been demonstrated that the integrated force method is equally viable and efficient as compared to the displacement method.

  18. LISA: a linear structured system analysis program

    Martinez-Martinez, Sinuhé; Mader, Theodor; Boukhobza, Taha; Hamelin, Frédéric


    International audience; In this paper the program LISA is presented. LISA is a flexible and portable program which has been developed to analyse structural properties of large scale linear and bilinear structured systems. More precisely, the program LISA contains programmed algorithms which allow us to apply recent results in the analysis of structured systems to some particular cases.

  19. Fourier Analysis and Structure Determination--Part III: X-ray Crystal Structure Analysis.

    Chesick, John P.


    Discussed is single crystal X-ray crystal structure analysis. A common link between the NMR imaging and the traditional X-ray crystal structure analysis is reported. Claims that comparisons aid in the understanding of both techniques. (MVL)

  20. Static Stiffness Modeling Method of3-RPS PKM%3-RPS并联机构静刚度建模方法

    落海伟; 张俊; 王辉; 黄田


    以三自由度并联动力头A3(3-RPS)为研究对象,提出了一种基于子结构综合和静态凝聚技术的静刚度建模和自重变形求解方法.建模过程中,将机构划分为动平台和RPS伸缩支链子结构,并综合考虑了铰链和支链弹性对静刚度的贡献.其中,球铰和转动副处理为具有等效刚度的集中质量虚拟弹簧;伸缩支链借助有限元软件和静态凝聚技术,建立了支链子结构质量矩阵和刚度矩阵;动平台处理为刚性质量块.通过引入变形协调条件,建立系统的整体刚度矩阵.研究结果表明,整机主刚度随机构位姿的变化而变化;在动平台连体坐标系下,3 个方向线刚度呈对称分布且 w 向刚度最大;运动部件自重会导致动平台末端产生较大弹性变形.该方法所得静刚度变化规律通过实测结果进行了验证.%A static stiffness modeling and weight deformation solving method for a three degree of freedom(3-DOF)parallel kinematic manipulator(PKM)A3 was proposed using the substructure synthesis and static condensation technique. During the modeling process,the whole system was divided into moving platform subsystem and three identical RPS limb subsystems,in which the contributions of the joint and limb compliances to the static stiffness were considered. In the stiffness model,the sphere joint and the revolute joint were treated as lumped virtual springs with equal stiffness,the platform was treated as a rigid body,and the structural mass matrix and the stiffnessmatrix of the RPS limbs were established using finite element software and static condensation technique. With the compati-bility conditions at interface between the limbs and the platform,an analytical governing dynamic equation of the system was then proposed. The simulation results show that the system stiffness vary with the changes of the configu-ration. In the coordinate system fixed to the moving platform,the distribution of the stiffness is axially

  1. Structural Analysis of the Upper Internal Structure in PGSFR

    Kim, S. H.; Koo, G. H. [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)


    The upper internal structure (UIS) is a package of hardware suspended from the rotating plug to about 20 cm above the core assemblies. The functions of the UIS are to support shroud tubes containing the primary and secondary control rod drivelines and preserve critical alignments between these drivelines and the core lattice, under normal and off-normal conditions. In addition, the UIS produces sufficient coolant mixing to mitigate thermal transients to downstream components and provides an opening for the In-Vessel transfer machine to access inner core positions without interfacing with the control rod drive lines and the upper core instrumentation package. The radial position of the shroud tube is fixed by three horizontal guide plates and the lower guide plate is close to the core assemblies and is perforated to permit most of the core effluent to reach the region between guide plates. In this study, the primary stress analysis for dead weight was carried out and the thermal stress analysis considering the coolant temperature around the UIS was performed. In addition, the mode characteristics of the structure by the natural frequency analysis were evaluated. The structural analysis model is developed to evaluate the structural integrity of the UIS. The primary stress analysis, the thermal stress analysis and the natural frequency analysis for the UIS are performed, and the maximum stresses and displacements are evaluated. From the analysis results, it is confirmed that the large local stresses don't occur near the holes and through the wall thicknesses of the structure. In addition, the maximum temperature of the UIS is calculated as 545 .deg. C from the thermal analysis and the structure should be evaluated by the ASME design rules at a high temperature. In the future, the more detailed design will be performed by the high temperature evaluation procedure according to the ASME SEC. III, Div.5.


    HUANG Yan; LAN Wei-ren


    Based on the nonlinear geometric relation between strain and displacement for flexible cable, the equilibrium equation under self-weight and influence of temperature was established and an analytical solution of displacement and tension distribution defined in Eulerian coordinate system was accurately obtained. The nonlinear algebraic equations caused by cable structure were solved directly using the modified Powell hybrid algorithm with high precision routine DNEQNE of Fortran. For example, a cable structure consisting of three cables jointly supported by a vertical spring and all the other ends fixed was calculated and compared with various methods by other scholars.




    Full Text Available The application of finite element method is analytical when solutions can not be applied for deeper study analyzes static, dynamic or other types of requirements in different points of the structures .In practice it is necessary to know the behavior of the structure or certain parts components of the machine under the influence of certain factors static and dynamic . The application of finite element in the optimization of components leads to economic growth , to increase reliability and durability organs studied, thus the machine itself.

  4. Probabilistic structural analysis by extremum methods

    Nafday, Avinash M.


    The objective is to demonstrate discrete extremum methods of structural analysis as a tool for structural system reliability evaluation. Specifically, linear and multiobjective linear programming models for analysis of rigid plastic frames under proportional and multiparametric loadings, respectively, are considered. Kinematic and static approaches for analysis form a primal-dual pair in each of these models and have a polyhedral format. Duality relations link extreme points and hyperplanes of these polyhedra and lead naturally to dual methods for system reliability evaluation.

  5. Analysis of composite structural elements

    A. Baier


    Full Text Available Purpose: The themes of the study are composite structural components. For this purpose have been designed and built several research positions.Design/methodology/approach: Using different structural materials to build new device components requires multiple tests of the components. Research posts were designed in the advanced graphical program CAx Siemens NX 7.5. Analysed samples were made from the glass fibre, aramid and carbon of various weights. Due to the specific use of composite materials it focuses on the elements in the form of plates and flat bars. For the examination of experimental strain gauge technique was used bead, the force sensor and displacement sensor. The experimental methods were compared with computer simulation using the FEM.Findings: The aim of this study was to determine the basic material constants and a comparison of the experimental method and the method of computer simulation.Research limitations/implications: Change the number of layers and how to connect the laminate with the steel plate changes mechanical properties of the structural component.Practical implications: The ultimate result will be knowledge on the different forms of laminates, such as material properties, the stresses in all layers, strain and comparing the results obtained by two methods.Originality/value: The expected outcome of the study will be the composition and method of joining composite laminate with a steel plate to the possible application in the repair and construction of structural elements of freight wagons.

  6. Structural analysis consultation using artificial intelligence

    Melosh, R. J.; Marcal, P. V.; Berke, L.


    The primary goal of consultation is definition of the best strategy to deal with a structural engineering analysis objective. The knowledge base to meet the need is designed to identify the type of numerical analysis, the needed modeling detail, and specific analysis data required. Decisions are constructed on the basis of the data in the knowledge base - material behavior, relations between geometry and structural behavior, measures of the importance of time and temperature changes - and user supplied specifics characteristics of the spectrum of analysis types, the relation between accuracy and model detail on the structure, its mechanical loadings, and its temperature states. Existing software demonstrated the feasibility of the approach, encompassing the 36 analysis classes spanning nonlinear, temperature affected, incremental analyses which track the behavior of structural systems.

  7. Theoretical studies on the influence of molecular interactions on the mechanism of electron transfer in photosynthetic reaction center of Rps. viridis

    徐红; 张汝波; 屈正旺; 张兴康; 张启元


    Based on the QM/MM optimized X-ray crystal structure of the photosynthetic reaction center (PRC) of purple bacteria Rhodopseudomonas (Rps.) viridis, quantum chemistry density functional method (DFT, B3LYP/6-31G) has been performed to study the interactions between the pigment molecules and either the surrounded amino acid residues or water molecules that are either axially coordinated or hydrogen bonded with the pigment molecules, leading to an explanation of the mechanism of the primary electron-transfer (ET) reactions in the PRC. Results show that the axial coordination of amino acid residues greatly raises the ELUMO of pigment molecules and it is important for the possibility of ET to take place. Different hydrogen bonds between amino acid residues, water molecules and pigment molecules decrease the ELUMO of the pigment molecules to different extents. It is crucial for the ET taking place from excited P along L branch and sustains that the ET is a one-step reaction without through accessory bacteriochlorophyll (ABChl b). It is insufficient to treat the whole protein surrounding as a homogeneous dielectric medium.

  8. Robustness Analysis of Timber Truss Structure

    Rajčić, Vlatka; Čizmar, Dean; Kirkegaard, Poul Henning


    The present paper discusses robustness of structures in general and the robustness requirements given in the codes. Robustness of timber structures is also an issues as this is closely related to Working group 3 (Robustness of systems) of the COST E55 project. Finally, an example of a robustness...... evaluation of a widespan timber truss structure is presented. This structure was built few years ago near Zagreb and has a span of 45m. Reliability analysis of the main members and the system is conducted and based on this a robustness analysis is preformed....

  9. Differentiation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis by specific PCR amplification of the RPS0 intron.

    del Pilar Vercher, M; García Martínez, José M; Cantón, Emilia; Pemán, Javier; Gómez García, M Micaela; Gómez, Eulogio Valentín; del Castillo Agudo, Lucas


    Although Candida parapsilosis is the most prevalent among the 3 species of the *psilosis group, studies applying DNA-based diagnostic techniques with isolates previously identified as C. parapsilosis have revealed that both C. orthopsilosis and C. metapsilosis account for 0-10% of all these isolates, depending on the geographical area. Differences in the degrees of antifungal susceptibility and virulence have been found, so a more precise identification is required. In a first approach, we reidentified 38 randomly chosen clinical isolates, previously identified as C. parapsilosis, using the RPO2 (CA2) RAPD marker. Among them, we reclassified 4 as C. metapsilosis and 5 as C. orthopsilosis. We previously developed a method to identify different pathogen yeast species, including C. parapsilosis, based on the amplification of the RPS0 gene intron. In this work, we extend this approach to the new *psilosis species by partially sequencing their RPS0 gene, including the intron sequence. Based on intron sequences, we designed specific primers capable of identifying C. orthopsilosis and C. metapsilosis species, and we reidentified species among the initial isolates. These new primers have allowed a specific and rapid identification of C. orthopsilosis and C. metapsilosis.

  10. Haploinsufficiency of RPS14 in 5q− syndrome is associated with deregulation of ribosomal- and translation-related genes

    Pellagatti, Andrea; Hellström-Lindberg, Eva; Giagounidis, Aristoteles; Perry, Janet; Malcovati, Luca; Della Porta, Matteo G; Jädersten, Martin; Killick, Sally; Fidler, Carrie; Cazzola, Mario; Wainscoat, James S; Boultwood, Jacqueline


    We have previously demonstrated haploinsufficiency of the ribosomal gene RPS14, which is required for the maturation of 40S ribosomal subunits and maps to the commonly deleted region, in the 5q− syndrome. Patients with Diamond-Blackfan anaemia (DBA) show haploinsufficiency of the closely related ribosomal protein RPS19, and show a consequent downregulation of multiple ribosomal- and translation-related genes. By analogy with DBA, we have investigated the expression profiles of a large group of ribosomal- and translation-related genes in the CD34+ cells of 15 myelodysplastic syndrome (MDS) patients with 5q− syndrome, 18 MDS patients with refractory anaemia (RA) and a normal karyotype, and 17 healthy controls. In this three-way comparison, 55 of 579 ribosomal- and translation-related probe sets were found to be significantly differentially expressed, with approximately 90% of these showing lower expression levels in the 5q− syndrome patient group. Using hierarchical clustering, patients with the 5q− syndrome could be separated both from other patients with RA and healthy controls solely on the basis of the deregulated expression of ribosomal- and translation-related genes. Patients with the 5q− syndrome have a defect in the expression of genes involved in ribosome biogenesis and in the control of translation, suggesting that the 5q− syndrome represents a disorder of aberrant ribosome biogenesis. PMID:18477045

  11. Analyses of wrky18 wrky40 plants reveal critical roles of SA/EDS1 signaling and indole-glucosinolate biosynthesis for Golovinomyces orontii resistance and a loss-of resistance towards Pseudomonas syringae pv. tomato AvrRPS4.

    Schön, Moritz; Töller, Armin; Diezel, Celia; Roth, Charlotte; Westphal, Lore; Wiermer, Marcel; Somssich, Imre E


    Simultaneous mutation of two WRKY-type transcription factors, WRKY18 and WRKY40, renders otherwise susceptible wild-type Arabidopsis plants resistant towards the biotrophic powdery mildew fungus Golovinomyces orontii. Resistance in wrky18 wrky40 double mutant plants is accompanied by massive transcriptional reprogramming, imbalance in salicylic acid (SA) and jasmonic acid (JA) signaling, altered ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) expression, and accumulation of the phytoalexin camalexin. Genetic analyses identified SA biosynthesis and EDS1 signaling as well as biosynthesis of the indole-glucosinolate 4MI3G as essential components required for loss-of-WRKY18 WRKY40-mediated resistance towards G. orontii. The analysis of wrky18 wrky40 pad3 mutant plants impaired in camalexin biosynthesis revealed an uncoupling of pre- from postinvasive resistance against G. orontii. Comprehensive infection studies demonstrated the specificity of wrky18 wrky40-mediated G. orontii resistance. Interestingly, WRKY18 and WRKY40 act as positive regulators in effector-triggered immunity, as the wrky18 wrky40 double mutant was found to be strongly susceptible towards the bacterial pathogen Pseudomonas syringae DC3000 expressing the effector AvrRPS4 but not against other tested Pseudomonas strains. We hypothesize that G. orontii depends on the function of WRKY18 and WRKY40 to successfully infect Arabidopsis wild-type plants while, in the interaction with P. syringae AvrRPS4, they are required to mediate effector-triggered immunity.

  12. Thermal and structural analysis of Hermes

    Petiau, C.


    After a brief recap of Hermes TPS and structure principles, we present the organization of thermal and structural analysis of the Hermes project, and we describe the way to resolve the problems of connections between calculations performed by the different Hermes partners. We describe in detail the interactions between the general model of TPS, used for global dimensioning of insulation, and refined thermal models giving an accurate temperature map inside details of "hot" and "cold" structures. The organization for structural analysis is based on a finite element general model which supports preliminary design, loads and vibration analyses. Boundary conditions for refined subpart analyses are cut to size, into the general model by a super element technique. This process involves the use by all partners of efficient computer codes, in the field of structural analysis and optimization integrated with CAD; for this Dassault proposes as a reference: the CATIA-ELFINI system.

  13. Dynamic analysis and design of offshore structures

    Chandrasekaran, Srinivasan


    This book  attempts to provide readers with an overall idea of various types of offshore platform geometries. It covers the various environmental loads encountered by these structures, a detailed description of the fundamentals of structural dynamics in a class-room style, estimate of damping in offshore structures and their applications in the preliminary analysis and design. Basic concepts of structural dynamics are emphasized through simple illustrative examples and exercises. Design methodologies and guidelines, which are FORM based concepts are explained through a few applied example structures. Each chapter also has tutorials and exercises for self-learning. A dedicated chapter on stochastic dynamics will help the students to extend the basic concepts of structural dynamics to this advanced domain of research. Hydrodynamic response of offshore structures with perforated members is one of the recent research applications, which is found to be one of the effective manner of retrofitting offshore structur...

  14. Semantic Antinomies and Deep Structure Analysis

    Zuber, Ryszard


    This article discusses constructions known as semantic antinomies, that is, the paradoxical results of false presuppositions, and how they can be dealt with by means of deep structure analysis. See FL 508 186 for availability. (CLK)

  15. Semantic Antinomies and Deep Structure Analysis

    Zuber, Ryszard


    This article discusses constructions known as semantic antinomies, that is, the paradoxical results of false presuppositions, and how they can be dealt with by means of deep structure analysis. See FL 508 186 for availability. (CLK)

  16. Structural Dynamics and Data Analysis

    Luthman, Briana L.


    This project consists of two parts, the first will be the post-flight analysis of data from a Delta IV launch vehicle, and the second will be a Finite Element Analysis of a CubeSat. Shock and vibration data was collected on WGS-5 (Wideband Global SATCOM- 5) which was launched on a Delta IV launch vehicle. Using CAM (CAlculation with Matrices) software, the data is to be plotted into Time History, Shock Response Spectrum, and SPL (Sound Pressure Level) curves. In this format the data is to be reviewed and compared to flight instrumentation data from previous flights of the same launch vehicle. This is done to ensure the current mission environments, such as shock, random vibration, and acoustics, are not out of family with existing flight experience. In family means the peaks on the SRS curve for WGS-5 are similar to the peaks from the previous flights and there are no major outliers. The curves from the data will then be compiled into a useful format so that is can be peer reviewed then presented before an engineering review board if required. Also, the reviewed data will be uploaded to the Engineering Review Board Information System (ERBIS) to archive. The second part of this project is conducting Finite Element Analysis of a CubeSat. In 2010, Merritt Island High School partnered with NASA to design, build and launch a CubeSat. The team is now called StangSat in honor of their mascot, the mustang. Over the past few years, the StangSat team has built a satellite and has now been manifested for flight on a SpaceX Falcon 9 launch in 2014. To prepare for the final launch, a test flight was conducted in Mojave, California. StangSat was launched on a Prospector 18D, a high altitude rocket made by Garvey Spacecraft Corporation, along with their sister satellite CP9 built by California Polytechnic University. However, StangSat was damaged during an off nominal landing and this project will give beneficial insights into what loads the CubeSat experienced during the crash

  17. Entity Authentication:Analysis using Structured Intuition

    Ahmed, Naveed; Jensen, Christian D.


    In this paper, we propose a new method for the analysis that uses intuition of the analyst in a structured way. First we define entity authentication in terms of fine level authentication goals (FLAGs). Then we use some relevant structures in protocol narrations and use them to justify FLAGs...... specification of security in terms of FLAGs; and secondly the outcome can be used to transform basic protocol narrations into more detailed specifications, which makes a subsequent formal analysis much more meaningful....

  18. Analysis model of structure-HDS


    Presents the model established for Structure-HDS(hydraulic damper system) analysis on the basis of the theoretical analysis model of non-compressed fluid in the round pipe will an uniform velocity used as the basic variable, and pressure losses resulting from cross section changes of fluid route taken into consideration. Which provides necessary basis for researches on earthquake responses of a structure with a spacious first story, equipped with HDS at first floor.

  19. Using rhetorical structure in sentiment analysis

    Hogenboom, Alexander; Frasincar, Flavius; Jong, de Franciska; Kaymak, Uzay


    Automated sentiment analysis has become an active field of study with a broad applicability. One of the key open research issues lies in dealing with structural aspects of text when analyzing its conveyed sentiment. Recent work uses structural aspects of text in order to distinguish important text s

  20. Generalized Structured Component Analysis with Latent Interactions

    Hwang, Heungsun; Ho, Moon-Ho Ringo; Lee, Jonathan


    Generalized structured component analysis (GSCA) is a component-based approach to structural equation modeling. In practice, researchers may often be interested in examining the interaction effects of latent variables. However, GSCA has been geared only for the specification and testing of the main effects of variables. Thus, an extension of GSCA…

  1. Group theory analysis of braided geometry structures

    FENG Wei; MA Wensuo


    The braided geometry structures are analyzed with point groups and space groups for which the continuous yarn of the braided preforms is segmented and expressed in some special symbols. All structures of braided material are described and classified with group theory, and new braiding methods are found. The group theory analysis lays the theoretical foundation for optimizing material performance.

  2. Posttranscriptional down-regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency.

    Badhai, Jitendra; Fröjmark, Anne-Sophie; Razzaghian, Hamid Reza; Davey, Edward; Schuster, Jens; Dahl, Niklas


    Ribosomal protein S19 (RPS19) is mutated in patients with Diamond-Blackfan anemia (DBA). We hypothesized that decreased levels of RPS19 lead to a coordinated down-regulation of other ribosomal (r-)proteins at the subunit level. We show that small interfering RNA (siRNA) knock-down of RPS19 results in a relative decrease of small subunit (SSU) r-proteins (S20, S21 and S24) when compared to large subunit (LSU) r-proteins (L3, L9, L30 and L38). This correlates with a relative decrease in 18S rRNA with respect to 28S rRNA. The r-protein mRNA levels remain relatively unchanged indicating a post transcriptional regulation of r-proteins at the level of subunit formation.

  3. Bayesian analysis of cosmic structures

    Kitaura, Francisco-Shu


    We revise the Bayesian inference steps required to analyse the cosmological large-scale structure. Here we make special emphasis in the complications which arise due to the non-Gaussian character of the galaxy and matter distribution. In particular we investigate the advantages and limitations of the Poisson-lognormal model and discuss how to extend this work. With the lognormal prior using the Hamiltonian sampling technique and on scales of about 4 h^{-1} Mpc we find that the over-dense regions are excellent reconstructed, however, under-dense regions (void statistics) are quantitatively poorly recovered. Contrary to the maximum a posteriori (MAP) solution which was shown to over-estimate the density in the under-dense regions we obtain lower densities than in N-body simulations. This is due to the fact that the MAP solution is conservative whereas the full posterior yields samples which are consistent with the prior statistics. The lognormal prior is not able to capture the full non-linear regime at scales ...

  4. Structural Analysis Of Offshore Structures Exposed To Blast Loads

    Hansen, Hans Jakup; Thygesen, Ulf; Kristensen, Anders;


    Numerical methods for simulations of blast loads and resulting structural response are investigated and compared to results obtained from tests. The CFD code EXSIM is used for the simulation of the blast load. This code provides a load profile wich is entered in the FEM analysis model....

  5. Structural Analysis in a Conceptual Design Framework

    Padula, Sharon L.; Robinson, Jay H.; Eldred, Lloyd B.


    Supersonic aircraft designers must shape the outer mold line of the aircraft to improve multiple objectives, such as mission performance, cruise efficiency, and sonic-boom signatures. Conceptual designers have demonstrated an ability to assess these objectives for a large number of candidate designs. Other critical objectives and constraints, such as weight, fuel volume, aeroelastic effects, and structural soundness, are more difficult to address during the conceptual design process. The present research adds both static structural analysis and sizing to an existing conceptual design framework. The ultimate goal is to include structural analysis in the multidisciplinary optimization of a supersonic aircraft. Progress towards that goal is discussed and demonstrated.

  6. Seismic analysis of nuclear power plant structures

    Go, J. C.


    Primary structures for nuclear power plants are designed to resist expected earthquakes of the site. Two intensities are referred to as Operating Basis Earthquake and Design Basis Earthquake. These structures are required to accommodate these seismic loadings without loss of their functional integrity. Thus, no plastic yield is allowed. The application of NASTRAN in analyzing some of these seismic induced structural dynamic problems is described. NASTRAN, with some modifications, can be used to analyze most structures that are subjected to seismic loads. A brief review of the formulation of seismic-induced structural dynamics is also presented. Two typical structural problems were selected to illustrate the application of the various methods of seismic structural analysis by the NASTRAN system.

  7. A protein structure data and analysis system.

    Tian, Hao; Sunderraman, Rajshekhar; Weber, Irene; Wang, Haibin; Yang, Hong


    In this paper, we present the design and implementation of a protein structure data and analysis system that is only used in the lab for analyzing the proprietary data. It is capable of storing public protein data, such as the data in Protein Data Bank (PDB) [1], and life scientists' proprietary data. This toolkit is targeted at life scientists who want to maintain proprietary protein structure data (may be incomplete), to search and query publicly known protein structures and to compare their structure data with others. The comparison functions can be used to find structure differences between two proteins at atom level, especially in mutant versions of proteins. The system can also be used as a tool of choosing better protein structure template in new protein's tertiary structure prediction. The system is developed in Java and the protein data is stored in a relational database (Oracle 9i).

  8. Polarity Analysis of Texts using Discourse Structure

    Heerschop, Bas; Goosen, Frank; Hogenboom, Alexander; Frasincar, Flavius; Kaymak, Uzay; Jong, de Franciska


    Sentiment analysis has applications in many areas and the exploration of its potential has only just begun. We propose Pathos, a framework which performs document sentiment analysis (partly) based on a document’s discourse structure. We hypothesize that by splitting a text into important and less im

  9. Structural-Thermal-Optical-Performance (STOP) Analysis

    Bolognese, Jeffrey; Irish, Sandra


    The presentation will be given at the 26th Annual Thermal Fluids Analysis Workshop (TFAWS 2015) hosted by the Goddard Spaceflight Center (GSFC) Thermal Engineering Branch (Code 545). A STOP analysis is a multidiscipline analysis, consisting of Structural, Thermal and Optical Performance Analyses, that is performed for all space flight instruments and satellites. This course will explain the different parts of performing this analysis. The student will learn how to effectively interact with each discipline in order to accurately obtain the system analysis results.

  10. Kinematic Analysis of a Hybrid Structure

    Q.J. Duan


    Full Text Available This paper presents a kinematic analysis and simulation of a hybrid structure applied to the new design cable-suspended feed structure (CSFS for the next generation of large spherical radio telescopes. First, considering the requirement that feeds should be tilted from 40° to 60° and that the tracking precision in steady state is 4mm, a novel design of the feed supporting structure including a cable-cabin structure, an AB axis structure and a Stewart platform is performed. Next, kinematic analysis and the simulation of the CSFS are done. Simulations have been developed in combination with the 50m CSFS model, which demonstrate the effectiveness and feasibility of the proposed three-level cable-suspended feed system.

  11. Structural Vibration Monitoring Using Cumulative Spectral Analysis

    Satoru Goto


    Full Text Available This paper describes a resonance decay estimation for structural health monitoring in the presence of nonstationary vibrations. In structural health monitoring, the structure's frequency response and resonant decay characteristics are very important for understanding how the structure changes. Cumulative spectral analysis (CSA estimates the frequency decay by using the impulse response. However, measuring the impulse response of buildings is impractical due to the need to shake the building itself. In a previous study, we reported on system damping monitoring using cumulative harmonic analysis (CHA, which is based on CSA. The current study describes scale model experiments on estimating the hidden resonance decay under non-stationary noise conditions by using CSA for structural condition monitoring.

  12. Advanced analysis for structural steel building design

    Wai Fah CHEN


    The 2005 AISC LRFD Specifications for Structural Steel Buildings are making it possible for designers to recognize explicitly the structural resistance provided within the elastic and inelastic ranges of beha-vior and up to the maximum load limit state. There is an increasing awareness of the need for practical second-order analysis approaches for a direct determination of overall structural system response. This paper attempts to present a simple, concise and reasonably comprehens-ive introduction to some of the theoretical and practical approaches which have been used in the traditional and modern processes of design of steel building structures.

  13. Temperature-induced dissociation reaction and dynamics of light-harvesting complex Ⅱ isolated from purple photosynthetic bacterium Rps. palustris

    FENG Juan; LI XueFeng; LIU Yuan


    Steady-state absorption spectroscopy, circular dichroism, and resonance Raman spectroscopy have been used to investigate the thermal stability of LH2 complex isolated from purple photosynthetic bacterium Rps. Palustris. The results show that: 1) upon increasing the temperature, a transition from B800 and B850 to free bacteriochlorophyll (B780) happens; 2) a gradual decrease and disappearance of CD signal in visible region occur; 3) a shift of the frequency, belonging to C=C and C-C stretching vibration, to higher wavenumber takes place. It is suggested that LH2 complex can be dissociated in the presence of B800, B850 and carotenoids simultaneously. Single-exponential fitting on the dynamic decay curves gives the apparent time constants of hundreds of minutes for various pigments.

  14. Single-molecule spectroscopy unmasks the lowest exciton state of the B850 assembly in LH2 from Rps. acidophila.

    Kunz, Ralf; Timpmann, Kõu; Southall, June; Cogdell, Richard J; Freiberg, Arvi; Köhler, Jürgen


    We have recorded fluorescence-excitation and emission spectra from single LH2 complexes from Rhodopseudomonas (Rps.) acidophila. Both types of spectra show strong temporal spectral fluctuations that can be visualized as spectral diffusion plots. Comparison of the excitation and emission spectra reveals that for most of the complexes the lowest exciton transition is not observable in the excitation spectra due to the cutoff of the detection filter characteristics. However, from the spectral diffusion plots we have the full spectral and temporal information at hand and can select those complexes for which the excitation spectra are complete. Correlating the red most spectral feature of the excitation spectrum with the blue most spectral feature of the emission spectrum allows an unambiguous assignment of the lowest exciton state. Hence, application of fluorescence-excitation and emission spectroscopy on the same individual LH2 complex allows us to decipher spectral subtleties that are usually hidden in traditional ensemble spectroscopy.

  15. Single nucleotide polymorphisms in the PRDX3 and RPS19 and risk of HPV persistence and cervical precancer/cancer.

    Mahboobeh Safaeian

    Full Text Available BACKGROUND: Host genetic factors might affect the risk of progression from infection with carcinogenic human papillomavirus (HPV, the etiologic agent for cervical cancer, to persistent HPV infection, and hence to cervical precancer and cancer. METHODOLOGY/PRINCIPAL FINDINGS: We assessed 18,310 tag single nucleotide polymorphisms (SNPs from 1113 genes in 416 cervical intraepithelial neoplasia 3 (CIN3/cancer cases, 356 women with persistent carcinogenic HPV infection (median persistence of 25 months and 425 randomly selected women (non-cases and non-HPV persistent from the 10,049 women from the Guanacaste, Costa Rica HPV natural history cohort. For gene and SNP associations, we computed age-adjusted odds ratio and p-trend. Three comparisons were made: 1 association with CIN3/cancer (compared CIN3/cancer cases to random controls, 2 association with persistence (compared HPV persistence to random controls, and 3 progression (compared CIN3/cancers with HPV-persistent group. Regions statistically significantly associated with CIN3/cancer included genes for peroxiredoxin 3 PRDX3, and ribosomal protein S19 RPS19. The single most significant SNPs from each gene associated with CIN3/cancer were PRDX3 rs7082598 (P(trend<0.0001, and RPS19 rs2305809 (P(trend=0.0007, respectively. Both SNPs were also associated with progression. CONCLUSIONS/SIGNIFICANCE: These data suggest involvement of two genes, RSP19 and PRDX3, or other SNPs in linkage disequilibrium, with cervical cancer risk. Further investigation showed that they may be involved in both the persistence and progression transition stages. Our results require replication but, if true, suggest a role for ribosomal dysfunction, mitochondrial processes, and/or oxidative stress, or other unknown function of these genes in cervical carcinogenesis.

  16. Analysis of flexible structures under lateral impact

    Ramirez, D. F. [Paul C. Kizzo and Associates Inc., Seismic Structural Group, Oakland, CA 94612 (United States); Razavi, H. [AREVA Inc., Civil Seismic Group, San Jose, CA 95119 (United States)


    Three methods for analysis of flexible structures under lateral impact are presented. The first proposed method (Method A) consists of: (1) modifying an available deceleration on a rigid target with conservation principles to account for structural flexibility; and (2) transient nonlinear analysis of the structure with the corrected forcing function. The second proposed method (Method B) is similar to Method A in obtaining the forcing function but it solves the equations of motion of an idealized two-degree-of-freedom system instead of directly using conservation principles. The last method simply provides the maximum force in the structure using the conservation of energy and linear momentum. A coupled simulation is also performed in LS-DYNA and compared against the proposed methods. A case study is presented to illustrate the applicability of all three methods and the LS-DYNA simulation. (authors)

  17. Dynamic Analysis of Structures Using Neural Networks

    N. Ahmadi


    Full Text Available In the recent years, neural networks are considered as the best candidate for fast approximation with arbitrary accuracy in the time consuming problems. Dynamic analysis of structures against earthquake has the time consuming process. We employed two kinds of neural networks: Generalized Regression neural network (GR and Back-Propagation Wavenet neural network (BPW, for approximating of dynamic time history response of frame structures. GR is a traditional radial basis function neural network while BPW categorized as a wavelet neural network. In BPW, sigmoid activation functions of hidden layer neurons are substituted with wavelets and weights training are achieved using Scaled Conjugate Gradient (SCG algorithm. Comparison the results of BPW with those of GR in the dynamic analysis of eight story steel frame indicates that accuracy of the properly trained BPW was better than that of GR and therefore, BPW can be efficiently used for approximate dynamic analysis of structures.

  18. Object-Oriented Analysis, Structured Analysis, and Jackson System Development

    Wieringa, R.J.; Van Assche, F.; Moulin, B.; Rolland, C.


    Conceptual modeling is the activity of producing a conceptual model of an actual or desired version of a universe of discourse (UoD). In this paper, two methods of conceptual modeling are compared, structured analysis (SA) and object-oriented analysis (OOA). This is done by transforming a model prod

  19. Object-Oriented Analysis, Structured Analysis, and Jackson System Development

    Van Assche, F.; Wieringa, Roelf J.; Moulin, B.; Rolland, C


    Conceptual modeling is the activity of producing a conceptual model of an actual or desired version of a universe of discourse (UoD). In this paper, two methods of conceptual modeling are compared, structured analysis (SA) and object-oriented analysis (OOA). This is done by transforming a model

  20. Data structures and algorithm analysis in Java

    Shaffer, Clifford A


    With its focus on creating efficient data structures and algorithms, this comprehensive text helps readers understand how to select or design the tools that will best solve specific problems. It uses Java as the programming language and is suitable for second-year data structure courses and computer science courses in algorithm analysis. Techniques for representing data are presented within the context of assessing costs and benefits, promoting an understanding of the principles of algorithm analysis and the effects of a chosen physical medium. The text also explores tradeoff issues, familiari

  1. Data structures and algorithm analysis in C++

    Shaffer, Clifford A


    With its focus on creating efficient data structures and algorithms, this comprehensive text helps readers understand how to select or design the tools that will best solve specific problems. It uses Microsoft C++ as the programming language and is suitable for second-year data structure courses and computer science courses in algorithm analysis.Techniques for representing data are presented within the context of assessing costs and benefits, promoting an understanding of the principles of algorithm analysis and the effects of a chosen physical medium. The text also explores tradeoff issues, f

  2. On the structural analysis of textile composites

    Bogdanovich, Alexander E.; Pastore, Christopher M.

    The local structural inhomogeneities which distinguish textile composites from laminated materials are discussed. Techniques for quantifying these inhomogeneities through three dimensional geometric modelling are introduced and methods of translating them into elastic properties are presented. Some basic ideas on application of spline functions to the stress field analysis in textile composites are proposed. The significance of internal continuity conditions for these materials is emphasized. Several analytical techniques based on the concept of a meso-volume are discussed. An example is presented to demonstrate the application of the method to structural analysis of textile composites.

  3. Value of Information Analysis in Structural Safety

    Konakli, Katerina; Faber, Michael Havbro


    of structural systems. In this context, experiments may refer to inspections or techniques of structural health monitoring. The Value of Information concept provides a powerful tool for determining whether the experimental cost is justified by the expected benefit and for identifying the optimal among different......Pre-posterior analysis can be used to assess the potential of an experiment to enhance decision making by providing information on parameters characterized by uncertainty. The present paper describes a framework for pre-posterior analysis for support of decisions related to maintenance...... and quality of information and the probabilistic dependencies between components of a system....

  4. RNA Secondary Structure Analysis Using RNAstructure.

    Mathews, David H


    RNAstructure is a user-friendly program for the prediction and analysis of RNA secondary structure. It is available as a Web server, as a program with a graphical user interface, or as a set of command-line tools. The programs are available for Microsoft Windows, Macintosh OS X, or Linux. This unit provides protocols for RNA secondary structure prediction (using the Web server or the graphical user interface) and prediction of high-affinity oligonucleotide biding sites to a structured RNA target (using the graphical user interface). Copyright © 2014 John Wiley & Sons, Inc.

  5. Improving transient analysis technology for aircraft structures

    Melosh, R. J.; Chargin, Mladen


    Aircraft dynamic analyses are demanding of computer simulation capabilities. The modeling complexities of semi-monocoque construction, irregular geometry, high-performance materials, and high-accuracy analysis are present. At issue are the safety of the passengers and the integrity of the structure for a wide variety of flight-operating and emergency conditions. The technology which supports engineering of aircraft structures using computer simulation is examined. Available computer support is briefly described and improvement of accuracy and efficiency are recommended. Improved accuracy of simulation will lead to a more economical structure. Improved efficiency will result in lowering development time and expense.

  6. Analysis of latent structures in linear systems

    Høskuldsson, Agnar


    that are useful, when studying latent structures. It is shown how loading weight vectors are generated and how they can be interpreted in analyzing the latent structure. It is shown how the covariance can be used to get useful ‘apriori’ information on the modeling task. Also some simple methods are presented...... to use for deciding if single or multiple latent structures should be used. The last part is about choosing the variables that should be used in the analysis. The traditional procedures to select variables to include in the model are presented and the insufficiencies of such approaches are demonstrated...

  7. Entity Authentication:Analysis using Structured Intuition

    Ahmed, Naveed; Jensen, Christian D.


    In this paper, we propose a new method for the analysis that uses intuition of the analyst in a structured way. First we define entity authentication in terms of fine level authentication goals (FLAGs). Then we use some relevant structures in protocol narrations and use them to justify FLAGs...... for the protocol. All along this process, we discover vulnerabilities and unstated assumptions of the protocol. As the method is intuition based, the quality of results depends on the expertise of the security analyst, however, the structured intuition has two major advantages: Firstly we get a precise...

  8. Homology modeling and analysis of structure predictions of the bovine rhinitis B virus RNA dependent RNA polymerase (RdRp).

    Rai, Devendra K; Rieder, Elizabeth


    Bovine Rhinitis B Virus (BRBV) is a picornavirus responsible for mild respiratory infection of cattle. It is probably the least characterized among the aphthoviruses. BRBV is the closest relative known to Foot and Mouth Disease virus (FMDV) with a ~43% identical polyprotein sequence and as much as 67% identical sequence for the RNA dependent RNA polymerase (RdRp), which is also known as 3D polymerase (3D(pol)). In the present study we carried out phylogenetic analysis, structure based sequence alignment and prediction of three-dimensional structure of BRBV 3D(pol) using a combination of different computational tools. Model structures of BRBV 3D(pol) were verified for their stereochemical quality and accuracy. The BRBV 3D(pol) structure predicted by SWISS-MODEL exhibited highest scores in terms of stereochemical quality and accuracy, which were in the range of 2Å resolution crystal structures. The active site, nucleic acid binding site and overall structure were observed to be in agreement with the crystal structure of unliganded as well as template/primer (T/P), nucleotide tri-phosphate (NTP) and pyrophosphate (PPi) bound FMDV 3D(pol) (PDB, 1U09 and 2E9Z). The closest proximity of BRBV and FMDV 3D(pol) as compared to human rhinovirus type 16 (HRV-16) and rabbit hemorrhagic disease virus (RHDV) 3D(pols) is also substantiated by phylogeny analysis and root-mean square deviation (RMSD) between C-α traces of the polymerase structures. The absence of positively charged α-helix at C terminal, significant differences in non-covalent interactions especially salt bridges and CH-pi interactions around T/P channel of BRBV 3D(pol) compared to FMDV 3D(pol), indicate that despite a very high homology to FMDV 3D(pol), BRBV 3D(pol) may adopt a different mechanism for handling its substrates and adapting to physiological requirements. Our findings will be valuable in the design of structure-function interventions and identification of molecular targets for drug design applicable

  9. Homology Modeling and Analysis of Structure Predictions of the Bovine Rhinitis B Virus RNA Dependent RNA Polymerase (RdRp

    Devendra K. Rai


    Full Text Available Bovine Rhinitis B Virus (BRBV is a picornavirus responsible for mild respiratory infection of cattle. It is probably the least characterized among the aphthoviruses. BRBV is the closest relative known to Foot and Mouth Disease virus (FMDV with a ~43% identical polyprotein sequence and as much as 67% identical sequence for the RNA dependent RNA polymerase (RdRp, which is also known as 3D polymerase (3Dpol. In the present study we carried out phylogenetic analysis, structure based sequence alignment and prediction of three-dimensional structure of BRBV 3Dpol using a combination of different computational tools. Model structures of BRBV 3Dpol were verified for their stereochemical quality and accuracy. The BRBV 3Dpol structure predicted by SWISS-MODEL exhibited highest scores in terms of stereochemical quality and accuracy, which were in the range of 2Å resolution crystal structures. The active site, nucleic acid binding site and overall structure were observed to be in agreement with the crystal structure of unliganded as well as template/primer (T/P, nucleotide tri-phosphate (NTP and pyrophosphate (PPi bound FMDV 3Dpol (PDB, 1U09 and 2E9Z. The closest proximity of BRBV and FMDV 3Dpol as compared to human rhinovirus type 16 (HRV-16 and rabbit hemorrhagic disease virus (RHDV 3Dpols is also substantiated by phylogeny analysis and root-mean square deviation (RMSD between C-α traces of the polymerase structures. The absence of positively charged α-helix at C terminal, significant differences in non-covalent interactions especially salt bridges and CH-pi interactions around T/P channel of BRBV 3Dpol compared to FMDV 3Dpol, indicate that despite a very high homology to FMDV 3Dpol, BRBV 3Dpol may adopt a different mechanism for handling its substrates and adapting to physiological requirements. Our findings will be valuable in the

  10. Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH

    Stuart I. Tsubota


    Full Text Available The protein enhancer of rudimentary homolog, ERH, is a small, highly conserved protein that has been found in animals, plants, and protists. Genetic and biochemical interactions have implicated ERH in the regulation of pyrimidine biosynthesis, DNA replication, transcription, mRNA splicing, cellular proliferation, tumorigenesis, and the Notch signaling pathway. In vertebrates and insects, ERH is nuclearly localized; however, an examination of the ERH amino-acid sequence does not reveal any nuclear localization signals. In this paper we show that the first 24 amino acids contain sequences necessary and sufficient for nuclear localization. Through yeast two-hybrid screens, three new binding partners of ERH, RPS3, RPL19, and DDIT4, were identified. RPS3 was isolated from both human and Drosophila screens. These interactions suggest functions of ERH in cell growth, cancer, and DNA repair. The ERH sequences necessary for the interactions between ERH and RPS3 and RPL19 are mapped onto the same 24-amino-acid region in ERH which are necessary for nuclear localization, suggesting that ERH is localizing to the nucleus through binding to one of its DNA-binding partners, such as RPS3 or RPL19.

  11. The rps4 locus as an alternative marker for barcoding bryophytes: evaluation based on data mining from GenBank%rps4作为苔藓植物候选条形码的可行性:基于GenBank数据的分析

    刘艳; 王建秀; 葛学军; 曹同


    Among the candidate DNA barcoding loci suggested for land plants, only rbcL and trnH-psbA are available for barcoding bryophytes. However, both loci have limitations in discriminating among species.The present study evaluated the feasibility of using the cpDNA rps4 locus as an additional marker to complement other candidate barcodes for bryophytes. We analyzed 3,365 rps4 sequences retrieved from GenBank using pair-wise distance and phylogenetic methods. Our results demonstrated the universality of rps4 in bryophytes; the locus covers 96% of moss families and 88% of liverwort families. The rps4 locus resolved 73.0% of the species we tested. The discriminatory ability of rps4 is better than that of rbcL-a in each of the six bryophyte genera (i.e. Plagiochila, Tortula, Plagiomnium, Pyrrhobryum, Pogonatum, Grimmia) most commonly represented in the database. Moreover, large numbers of rps4 sequences from individuals of known bryophyte identities have been compiled in GenBank, thereby providing a reference for species identification. Therefore, we propose rps4 as an additional barcode, especially when rbcL and trnH-psbA do not perform well in certain bryophyte taxa.%对于苔藓植物DNA条形码研究来说,目前已提议的可用片段只有rbcL和trnH-psbA,并且均具有一定局限性.本文基于GenBank中3,365条rps4序列,利用遗传距离法和分子系统学方法评价它作为苔藓植物候选条形码的可行性.结果显示:(1)rps4序列覆盖了藓纲96%的科和苔纲88%的科,具有通用性;(2)rps4物种分辨能力为73.0%,并且它在6个序列最丰富的苔藓植物属(Plagiochila,Tortula,Plagiomnium,Pyrrhobryum,Pogonatum,Grimmia)内的物种识别能力均高于rbcL-a在同属中的分辨能力;(3)GenBank中已经积累了大量已知物种米源的rps4序列,可为DNA条形码物种鉴定提供一个参考数据库.因此,本文建议将rps4作为苔藓植物候选DNA条形码.尤其是当rbcL和trnH-psbA在某个具体类群中无法

  12. Term Structure Analysis with Big Data

    Andreasen, Martin Møller; Christensen, Jens H.E.; Rudebusch, Glenn D.

    Analysis of the term structure of interest rates almost always takes a two-step approach. First, actual bond prices are summarized by interpolated synthetic zero-coupon yields, and second, a small set of these yields are used as the source data for further empirical examination. In contrast, we...

  13. A Prospective Analysis of the Costs, Benefits, and Impacts of U.S. Renewable Portfolio Standards

    Mai, Trieu [National Renewable Energy Lab. (NREL), Golden, CO (United States); Wiser, Ryan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Barbose, Galen [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Bird, Lori [National Renewable Energy Lab. (NREL), Golden, CO (United States); Heeter, Jenny [National Renewable Energy Lab. (NREL), Golden, CO (United States); Keyser, David [National Renewable Energy Lab. (NREL), Golden, CO (United States); Krishnan, Venkat [National Renewable Energy Lab. (NREL), Golden, CO (United States); Macknick, Jordan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Millstein, Dev [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)


    As states have gained experience with renewable portfolio standards (RPS) policies, many have made significant revisions to existing programs. In 2015 and 2016, seven states raised and extended their final RPS targets, while another state enacted a new RPS policy (Barbose 2016b). Interest in expanding and strengthening state RPS programs may continue, while efforts like recent proposals in many states to repeal or freeze existing RPS policies may also persist. In either context, questions about the potential costs, benefits, and other impacts of RPS programs are usually central to the decision-making process. This report follows on previous analyses that have focused on the historical costs, benefits, and other impacts of existing state RPS programs (Heeter et al. 2014; Wiser et al. 2016a). This report examines RPS outcomes prospectively, considering both current RPS policies as well as a potential expansion of those policies. The goal of this work is to provide a consistent and independent analytical methodology for that examination. This analysis relies on National Renewable Energy Laboratory’s (NREL’s) Regional Energy Deployment System (ReEDS) model to estimate changes to the U.S. electric power sector across a number of scenarios and sensitivity cases, focusing on the 2015–2050 timeframe. Based on those modeled results, we evaluate the costs, benefits, and other impacts of renewable energy contributing to RPS compliance using the suite of methods employed in a number of recent studies sponsored by the U.S. Department of Energy (DOE): a report examining retrospective benefits and impacts of RPS programs (Wiser et al. 2016a), the Wind Vision report (DOE 2015), the On the Path to SunShot report focusing on environmental benefits (Wiser et al. 2016b), and the Hydropower Vision report (DOE 2016).

  14. A Dynamic Model for Energy Structure Analysis


    Energy structure is a complicated system concerning economic development, natural resources, technological innovation, ecological balance, social progress and many other elements. It is not easy to explain clearly the developmental mechanism of an energy system and the mutual relations between the energy system and its related environments by the traditional methods. It is necessary to develop a suitable dynamic model, which can reflect the dynamic characteristics and the mutual relations of the energy system and its related environments. In this paper, the historical development of China's energy structure was analyzed. A new quantitative analysis model was developed based on system dynamics principles through analysis of energy resources, and the production and consumption of energy in China and comparison with the world. Finally, this model was used to predict China's future energy structures under different conditions.

  15. Structural Analysis of Molecular Clouds: Dendrograms

    Rosolowsky, E W; Kauffmann, J; Goodman, A A


    We demonstrate the utility of dendrograms at representing the essential features of the hierarchical structure of the isosurfaces for molecular line data cubes. The dendrogram of a data cube is an abstraction of the changing topology of the isosurfaces as a function of contour level. The ability to track hierarchical structure over a range of scales makes this analysis philosophically different from local segmentation algorithms like CLUMPFIND. Points in the dendrogram structure correspond to specific volumes in data cubes defined by their bounding isosurfaces. We further refine the technique by measuring the properties associated with each isosurface in the analysis allowing for a multiscale calculation of molecular gas properties. Using COMPLETE 13CO(1-0) data from the L1448 region in Perseus and mock observations of a simulated data cube, we identify regions that have a significant contribution by self-gravity to their energetics on a range of scales. We find evidence for self-gravitation on all spatial sc...

  16. 面向XYZ-3RPS混联机床的后置处理算法%Post-processing algorithm oriented to XYZ-3RPS serial-parallel machine tool

    王瑞; 张庆彪


    Aiming at eliminating the disadvantage in current post⁃processing system which cannot generate the CNC instruction for serial⁃parallel machine tool, post⁃processing algorithm, fit for this sort of processing device, was discussed based on XYZ⁃3RPS serial⁃parallel machine tool model. By the analysis of structure and post⁃processing process of the serial⁃parallel machine tool, the key algorithm of post⁃processing which includes interpolation algorithm for cutter bit data under Cartesian coordinates, the inverse solution under coordinate of machine tool and the interference checking algorithm were proposed. Meanwhile, some unqualified cutter bit positions were corrected and cutter location data files had been changed into machine tool driver files. Finally, the validity of the algorithm was verified by the processing experiments of vane and sphere.%针对现有的后置处理系统无法直接生成混联机床数控指令的问题,以XYZ -3RPS混联机床为模型,研究适合该加工装置的后置处理算法。对混联机床的结构以及后置处理过程进行分析,提出后置处理的关键算法:包括工件坐标系下刀位数据的双直线插补算法,机床坐标系下刀位数据的逆解算法以及混联装置的干涉检查算法,对不满足条件的刀位点进行相应的处理,实现将刀位数据文件转化为混联机床的驱动文件。叶片和球面加工实例验证了算法正确性和有效性。

  17. Retrospective Analysis of the Benefits and Impacts of U.S. Renewable Portfolio Standards

    Wiser, Ryan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Barbose, Galen [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Heeter, Jenny [National Renewable Energy Lab. (NREL), Golden, CO (United States); Mai, Trieu [National Renewable Energy Lab. (NREL), Golden, CO (United States); Bird, Lori [National Renewable Energy Lab. (NREL), Golden, CO (United States); Bolinger, Mark [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Carpenter, Alberta [National Renewable Energy Lab. (NREL), Golden, CO (United States); Heath, Garvin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Keyser, David [National Renewable Energy Lab. (NREL), Golden, CO (United States); Macknick, Jordan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Mills, Andrew [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Millstein, Dev [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)


    This analysis is the first-ever comprehensive assessment of the benefits and impacts of state renewable portfolio standards (RPSs). This joint National Renewable Energy Laboratory-Lawrence Berkeley National Laboratory project provides a retrospective analysis of RPS program benefits and impacts, including greenhouse gas emissions reductions, air pollution emission reductions, water use reductions, gross jobs and economic development impacts, wholesale electricity price reduction impacts, and natural gas price reduction impacts. Wherever possible, benefits and impacts are quantified in monetary terms. The paper will inform state policymakers, RPS program administrators, industry, and others about the costs and benefits of state RPS programs. In particular, the work seeks to inform decision-making surrounding ongoing legislative proposals to scale back, freeze, or expand existing RPS programs, as well as future discussions about increasing RPS targets or otherwise increasing renewable energy associated with Clean Power Plan compliance or other emission-reduction goals.

  18. Retrospective Analysis of the Benefits and Impacts of U.S. Renewable Portfolio Standards

    Wiser, Ryan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Barbose, Galen [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Heeter, Jenny [National Renewable Energy Lab. (NREL), Golden, CO (United States); Mai, Trieu [National Renewable Energy Lab. (NREL), Golden, CO (United States); Bird, Lori [National Renewable Energy Lab. (NREL), Golden, CO (United States); Bolinger, Mark [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Carpenter, Alberta [National Renewable Energy Lab. (NREL), Golden, CO (United States); Heath, Garvin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Keyser, David [National Renewable Energy Lab. (NREL), Golden, CO (United States); Macknick, Jordan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Mills, Andrew [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Millstein, Dev [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)


    This analysis is the first-ever comprehensive assessment of the benefits and impacts of state renewable portfolio standards (RPSs). This joint National Renewable Energy Laboratory-Lawrence Berkeley National Laboratory project provides a retrospective analysis of RPS program benefits and impacts, including greenhouse gas emissions reductions, air pollution emission reductions, water use reductions, gross jobs and economic development impacts, wholesale electricity price reduction impacts, and natural gas price reduction impacts. Wherever possible, benefits and impacts are quantified in monetary terms. The paper will inform state policymakers, RPS program administrators, industry, and others about the costs and benefits of state RPS programs. In particular, the work seeks to inform decision-making surrounding ongoing legislative proposals to scale back, freeze, or expand existing RPS programs, as well as future discussions about increasing RPS targets or otherwise increasing renewable energy associated with Clean Power Plan compliance or other emission-reduction goals.

  19. Segmentation of histological structures for fractal analysis

    Dixon, Vanessa; Kouznetsov, Alexei; Tambasco, Mauro


    Pathologists examine histology sections to make diagnostic and prognostic assessments regarding cancer based on deviations in cellular and/or glandular structures. However, these assessments are subjective and exhibit some degree of observer variability. Recent studies have shown that fractal dimension (a quantitative measure of structural complexity) has proven useful for characterizing structural deviations and exhibits great potential for automated cancer diagnosis and prognosis. Computing fractal dimension relies on accurate image segmentation to capture the architectural complexity of the histology specimen. For this purpose, previous studies have used techniques such as intensity histogram analysis and edge detection algorithms. However, care must be taken when segmenting pathologically relevant structures since improper edge detection can result in an inaccurate estimation of fractal dimension. In this study, we established a reliable method for segmenting edges from grayscale images. We used a Koch snowflake, an object of known fractal dimension, to investigate the accuracy of various edge detection algorithms and selected the most appropriate algorithm to extract the outline structures. Next, we created validation objects ranging in fractal dimension from 1.3 to 1.9 imitating the size, structural complexity, and spatial pixel intensity distribution of stained histology section images. We applied increasing intensity thresholds to the validation objects to extract the outline structures and observe the effects on the corresponding segmentation and fractal dimension. The intensity threshold yielding the maximum fractal dimension provided the most accurate fractal dimension and segmentation, indicating that this quantitative method could be used in an automated classification system for histology specimens.

  20. Stochastic Simulation Tool for Aerospace Structural Analysis

    Knight, Norman F.; Moore, David F.


    Stochastic simulation refers to incorporating the effects of design tolerances and uncertainties into the design analysis model and then determining their influence on the design. A high-level evaluation of one such stochastic simulation tool, the MSC.Robust Design tool by MSC.Software Corporation, has been conducted. This stochastic simulation tool provides structural analysts with a tool to interrogate their structural design based on their mathematical description of the design problem using finite element analysis methods. This tool leverages the analyst's prior investment in finite element model development of a particular design. The original finite element model is treated as the baseline structural analysis model for the stochastic simulations that are to be performed. A Monte Carlo approach is used by MSC.Robust Design to determine the effects of scatter in design input variables on response output parameters. The tool was not designed to provide a probabilistic assessment, but to assist engineers in understanding cause and effect. It is driven by a graphical-user interface and retains the engineer-in-the-loop strategy for design evaluation and improvement. The application problem for the evaluation is chosen to be a two-dimensional shell finite element model of a Space Shuttle wing leading-edge panel under re-entry aerodynamic loading. MSC.Robust Design adds value to the analysis effort by rapidly being able to identify design input variables whose variability causes the most influence in response output parameters.



    The form of the modal analysis of viscoelastically damped structures is simplified and this simplified form is similar to the form of the modal analysis of linear viscously damped structures. As a result of this simplified form, the experimental modal analysis methods of linear viscously damped structures are applied to the experimental modal analysis of viscoelastically damped structures.

  2. Static Structural and Modal Analysis Using Isogeometric Analysis

    Gondegaon, Sangamesh; Voruganti, Hari K.


    Isogeometric Analysis (IGA) is a new analysis method for unification of Computer Aided Design (CAD) and Computer Aided Engineering (CAE). With the use of NURBS basis functions for both modelling and analysis, the bottleneck of meshing is avoided and a seamless integration is achieved. The CAD and computational geometry concepts in IGA are new to the analysis community. Though, there is a steady growth of literature, details of calculations, explanations and examples are not reported. The content of the paper is complimentary to the existing literature and addresses the gaps. It includes summary of the literature, overview of the methodology, step-by-step calculations and Matlab codes for example problems in static structural and modal analysis in 1-D and 2-D. At appropriate places, comparison with the Finite Element Analysis (FEM) is also included, so that those familiar with FEM can appreciate IGA better.

  3. Combination of structural reliability and interval analysis

    Zhiping Qiu; Di Yang; saac Elishakoff


    In engineering applications,probabilistic reliability theory appears to be presently the most important method,however,in many cases precise probabilistic reliability theory cannot be considered as adequate and credible model of the real state of actual affairs.In this paper,we developed a hybrid of probabilistic and non-probabilistic reliability theory,which describes the structural uncertain parameters as interval variables when statistical data are found insufficient.By using the interval analysis,a new method for calculating the interval of the structural reliability as well as the reliability index is introduced in this paper,and the traditional probabilistic theory is incorporated with the interval analysis.Moreover,the new method preserves the useful part of the traditional probabilistic reliability theory,but removes the restriction of its strict requirement on data acquisition.Example is presented to demonstrate the feasibility and validity of the proposed theory.

  4. Numerical Limit Analysis of Reinforced Concrete Structures

    Larsen, Kasper Paaske

    methods provide engineers with valuable tools for limit sta- te analysis, their application becomes difficult with increased structural complexity. The main challenge is to solve the optimization problem posed by the extremum principles. This thesis is a study of how numerical methods can be used to solve...... limit state analysis problems. The work focuses on determination of the load bearing capacity of reinforced concrete structures by employing the lower bound theorem and a finite element method using equilibrium elements is developed. The recent year’s development within the field of convex optimization...... is developed for improved perfor- mance. An example is given in which an inverse T-beam is analyzed and the numerical results are compared to laboratory tests. The third and final element is a plane shell element capable of modeling membrane and plate bending behavior. The element employs a layered disk...

  5. Reliability Analysis of Elasto-Plastic Structures


    . Failure of this type of system is defined either as formation of a mechanism or by failure of a prescribed number of elements. In the first case failure is independent of the order in which the elements fail, but this is not so by the second definition. The reliability analysis consists of two parts...... are described and the two definitions of failure can be used by the first formulation, but only the failure definition based on formation of a mechanism by the second formulation. The second part of the reliability analysis is an estimate of the failure probability for the structure on the basis...... are obtained if the failure mechanisms are used. Lower bounds can be calculated on the basis of series systems where the elements are the non-failed elements in a non-failed structure (see Augusti & Baratta [3])....

  6. Limit analysis of solid reinforced concrete structures

    Larsen, Kasper Paaske


    element for lower bound analysis of reinforced concrete structures is presented. The method defines the stress state at a point within the solid as a combination of concrete- and reinforcement stresses and yield criterions are applied to the stress components separately. This method allows for orthotropic......Recent studies have shown that Semidefinite Programming (SDP) can be used effectively for limit analysis of isotropic cohesive-frictional continuums using the classical Mohr-Coulomb yield criterion. In this paper we expand on this previous research by adding reinforcement to the model and a solid...... reinforcement and it is therefore possible to analyze structures with complex reinforcement layouts. Tests are conducted to validate the method against well-known analytical solutions....


    HURDUZEU Gheorghe


    taxes on income of corporations and taxes on income of individuals and households and other current taxes. Actual social contributions were also split into employer’s actual contributions, employee’s social contributions and social contributions of self- and non-employed persons. As the primary data analysis revealed many differences between Euro Area member states, but also similarities concerning various fiscal aggregates, we completed the analysis through multidimensional analysis, with the aims of classifying Euro Area member states into subgroups with similar fiscal structures. Taking into consideration the above mentioned variables, we used cluster analysis in order to determine which member states have similar fiscal structures and which are the main similarities that characterize Euro Area in this respect.

  8. Non Linear Seismic Analysis of Masonry Structures

    Sirajuddin, M


    Full Text Available Nowadays, even though many new construction techniques have been introduced, masonry has got its own importance in building industry. Masonry structures fail miserably under lateral loading conditions like earth quakes and impact loads. The occurrence of recent earthquakes in India and in different parts of the world have highlighted that most of the loss of human lives and damage to property have been due to the collapse of masonry structures. Though an earthquake could not be prevented, the loss of life and property could be minimized, if necessary steps could be taken to reduce the damages on the existing masonry structures. This paper investigates the application ofNonlinear Seismic Analysis of a masonry building using ANSYS software and check the efficacy of retrofit measuresto protect the existing building.

  9. MBS Analysis Of Kinetic Structures Using ADAMS

    Kirkegaard, Poul Henning; Nielsen, Søren R.K.


    The present paper considers multibody system (MBS) analysis of kinetic structures using the software package ADAMS. Deployable, foldable, expandable and reconfigurable kinetic structures can provide a change in the geometric morphology of the envelope by contributing to making it adaptable to e.......g. changing external climate factors, in order to improve the indoor climate performance of the building. The derivation of equations of motion for such spatial mechanical systems is a challenging issue in scientific community. However, with new symbolic tools one can automatically derive equations in so......-called multibody system (MBS) formalism. The present paper considers MBS modeling of kinetic architectural structures using the software packages ADAMS. As a result, it is found that symbolic MBS simulation tools facilitate a useful evaluation environment for MBS users during a design phase of responsive kinetic...

  10. Linking advanced fracture models to structural analysis

    Chiesa, Matteo


    Shell structures with defects occur in many situations. The defects are usually introduced during the welding process necessary for joining different parts of the structure. Higher utilization of structural materials leads to a need for accurate numerical tools for reliable prediction of structural response. The direct discretization of the cracked shell structure with solid finite elements in order to perform an integrity assessment of the structure in question leads to large size problems, and makes such analysis infeasible in structural application. In this study a link between local material models and structural analysis is outlined. An ''ad hoc'' element formulation is used in order to connect complex material models to the finite element framework used for structural analysis. An improved elasto-plastic line spring finite element formulation, used in order to take cracks into account, is linked to shell elements which are further linked to beam elements. In this way one obtain a global model of the shell structure that also accounts for local flexibilities and fractures due to defects. An important advantage with such an approach is a direct fracture mechanics assessment e.g. via computed J-integral or CTOD. A recent development in this approach is the notion of two-parameter fracture assessment. This means that the crack tip stress tri-axiality (constraint) is employed in determining the corresponding fracture toughness, giving a much more realistic capacity of cracked structures. The present thesis is organized in six research articles and an introductory chapter that reviews important background literature related to this work. Paper I and II address the performance of shell and line spring finite elements as a cost effective tool for performing the numerical calculation needed to perform a fracture assessment. In Paper II a failure assessment, based on the testing of a constraint-corrected fracture mechanics specimen under tension, is

  11. TLP hull structural design and analysis

    Yang Jiahui; Ren Michael


    Tension leg platform (TLP) has been one of the most favorite deep-water platform concepts for offshore oil and gas field exploration and development.As of now,a total of 24 TLPs have been installed worldwide with 3 more to be installed in the near future and 5 more under design.Most of these installations are in the Gulf of Mexico (GoM).Water depths for these TLP installations range from 150 m to 1 600 m.It is highly expected that China will have her first TLP designed,fabricated,and installed in the very near future.In order to satisfy the need for a unified hull structural design practice,this paper presents the design philosophy of a conventional TLP hull structure with emphases on critical structural components design and analysis methodologies.

  12. A Structural Analysis of European Union Economy

    Liliana DUGULEANĂ


    Full Text Available Based on the input-output analysis, the paper characterizes the structure of European economy in 2010 and 2011, on six main economic sectors. The sectoral structures of backward and forward linkages, and their absolute effects starting from the final demand, were compared with the real structures of sectors output, sectoral imports, sectoral value added, in 2011 face to 2010. The changes in final demand and in sectoral output can be analyzed in the propagation processes of the inter-sectoral economic flows, and allow us to know the behavior at macroeconomic level of European economy. The common economic policies at European level can be undertaken to keep the equilibrium between different sectors, to stimulate the sectors with high levels of productivity, to ensure the efficiency of using the resources, and the sustainability of economic development, which are the purposes of a smart development.

  13. Structural Analysis About a New Solar Sail

    ZHENG Jinjin; SHENG Zhaoyu; ZHOU Hongjun; HUANG Wenhao; SHENG Lianguan


    The article presents a structural analysis of a new space probe-solar sail. It was deployed successfully on ground. The loads for an outer space mission was introduced and expressed with equation. As a special state, the largest load around earth was used to analyze the model by the finite element method. Some results about strain and stress was obtained after setting some initial parameters. Compared to the results in the literature, the results presented here are significant.

  14. Structural analysis at aircraft conceptual design stage

    Mansouri, Reza

    In the past 50 years, computers have helped by augmenting human efforts with tremendous pace. The aircraft industry is not an exception. Aircraft industry is more than ever dependent on computing because of a high level of complexity and the increasing need for excellence to survive a highly competitive marketplace. Designers choose computers to perform almost every analysis task. But while doing so, existing effective, accurate and easy to use classical analytical methods are often forgotten, which can be very useful especially in the early phases of the aircraft design where concept generation and evaluation demands physical visibility of design parameters to make decisions [39, 2004]. Structural analysis methods have been used by human beings since the very early civilization. Centuries before computers were invented; the pyramids were designed and constructed by Egyptians around 2000 B.C, the Parthenon was built by the Greeks, around 240 B.C, Dujiangyan was built by the Chinese. Persepolis, Hagia Sophia, Taj Mahal, Eiffel tower are only few more examples of historical buildings, bridges and monuments that were constructed before we had any advancement made in computer aided engineering. Aircraft industry is no exception either. In the first half of the 20th century, engineers used classical method and designed civil transport aircraft such as Ford Tri Motor (1926), Lockheed Vega (1927), Lockheed 9 Orion (1931), Douglas DC-3 (1935), Douglas DC-4/C-54 Skymaster (1938), Boeing 307 (1938) and Boeing 314 Clipper (1939) and managed to become airborne without difficulty. Evidencing, while advanced numerical methods such as the finite element analysis is one of the most effective structural analysis methods; classical structural analysis methods can also be as useful especially during the early phase of a fixed wing aircraft design where major decisions are made and concept generation and evaluation demands physical visibility of design parameters to make decisions

  15. Remote geologic structural analysis of Yucca Flat

    Foley, M.G.; Heasler, P.G.; Hoover, K.A. (Pacific Northwest Lab., Richland, WA (United States)); Rynes, N.J. (Northern Illinois Univ., De Kalb, IL (United States)); Thiessen, R.L.; Alfaro, J.L. (Washington State Univ., Pullman, WA (United States))


    The Remote Geologic Analysis (RGA) system was developed by Pacific Northwest Laboratory (PNL) to identify crustal structures that may affect seismic wave propagation from nuclear tests. Using automated methods, the RGA system identifies all valleys in a digital elevation model (DEM), fits three-dimensional vectors to valley bottoms, and catalogs all potential fracture or fault planes defined by coplanar pairs of valley vectors. The system generates a cluster hierarchy of planar features having greater-than-random density that may represent areas of anomalous topography manifesting structural control of erosional drainage development. Because RGA uses computer methods to identify zones of hypothesized control of topography, ground truth using a well-characterized test site was critical in our evaluation of RGA's characterization of inaccessible test sites for seismic verification studies. Therefore, we applied RGA to a study area centered on Yucca Flat at the Nevada Test Site (NTS) and compared our results with both mapped geology and geologic structures and with seismic yield-magnitude models. This is the final report of PNL's RGA development project for peer review within the US Department of Energy Office of Arms Control (OAC) seismic-verification community. In this report, we discuss the Yucca Flat study area, the analytical basis of the RGA system and its application to Yucca Flat, the results of the analysis, and the relation of the analytical results to known topography, geology, and geologic structures. 41 refs., 39 figs., 2 tabs.

  16. Remote geologic structural analysis of Yucca Flat

    Foley, M.G.; Heasler, P.G.; Hoover, K.A. [Pacific Northwest Lab., Richland, WA (United States); Rynes, N.J. [Northern Illinois Univ., De Kalb, IL (United States); Thiessen, R.L.; Alfaro, J.L. [Washington State Univ., Pullman, WA (United States)


    The Remote Geologic Analysis (RGA) system was developed by Pacific Northwest Laboratory (PNL) to identify crustal structures that may affect seismic wave propagation from nuclear tests. Using automated methods, the RGA system identifies all valleys in a digital elevation model (DEM), fits three-dimensional vectors to valley bottoms, and catalogs all potential fracture or fault planes defined by coplanar pairs of valley vectors. The system generates a cluster hierarchy of planar features having greater-than-random density that may represent areas of anomalous topography manifesting structural control of erosional drainage development. Because RGA uses computer methods to identify zones of hypothesized control of topography, ground truth using a well-characterized test site was critical in our evaluation of RGA`s characterization of inaccessible test sites for seismic verification studies. Therefore, we applied RGA to a study area centered on Yucca Flat at the Nevada Test Site (NTS) and compared our results with both mapped geology and geologic structures and with seismic yield-magnitude models. This is the final report of PNL`s RGA development project for peer review within the US Department of Energy Office of Arms Control (OAC) seismic-verification community. In this report, we discuss the Yucca Flat study area, the analytical basis of the RGA system and its application to Yucca Flat, the results of the analysis, and the relation of the analytical results to known topography, geology, and geologic structures. 41 refs., 39 figs., 2 tabs.

  17. Industrial entrepreneurial network: Structural and functional analysis

    Medvedeva, M. A.; Davletbaev, R. H.; Berg, D. B.; Nazarova, J. J.; Parusheva, S. S.


    Structure and functioning of two model industrial entrepreneurial networks are investigated in the present paper. One of these networks is forming when implementing an integrated project and consists of eight agents, which interact with each other and external environment. The other one is obtained from the municipal economy and is based on the set of the 12 real business entities. Analysis of the networks is carried out on the basis of the matrix of mutual payments aggregated over the certain time period. The matrix is created by the methods of experimental economics. Social Network Analysis (SNA) methods and instruments were used in the present research. The set of basic structural characteristics was investigated: set of quantitative parameters such as density, diameter, clustering coefficient, different kinds of centrality, and etc. They were compared with the random Bernoulli graphs of the corresponding size and density. Discovered variations of random and entrepreneurial networks structure are explained by the peculiarities of agents functioning in production network. Separately, were identified the closed exchange circuits (cyclically closed contours of graph) forming an autopoietic (self-replicating) network pattern. The purpose of the functional analysis was to identify the contribution of the autopoietic network pattern in its gross product. It was found that the magnitude of this contribution is more than 20%. Such value allows using of the complementary currency in order to stimulate economic activity of network agents.

  18. Analysis of waveguiding properties of VCSEL structures

    Erteza, I.A. [Sandia National Labs., Albuquerque, NM (United States). Exploratory Systems Development Center


    In this paper, the authors explore the feasibility of using the distributed Bragg reflector, grown on the substrate for a VCSEL (Vertical Cavity Surface Emitting Laser), to provide waveguiding within the substrate. This waveguiding could serve as an interconnection among VCSELs in an array. Before determining the feasibility of waveguide interconnected VCSELs, two analysis methods are presented and evaluated for their applicability to this problem. The implementations in Mathematica of both these methods are included. Results of the analysis show that waveguiding in VCSEL structures is feasible. Some of the many possible uses of waveguide interconnected VCSELs are also briefly discussed. The tools and analysis presented in this report can be used to evaluate such system concepts and to do detailed design calculations.

  19. Zfrp8/PDCD2 Interacts with RpS2 Connecting Ribosome Maturation and Gene-Specific Translation.

    Svetlana Minakhina

    Full Text Available Zfrp8/PDCD2 is a highly conserved protein essential for stem cell maintenance in both flies and mammals. It is also required in fast proliferating cells such as cancer cells. Our previous studies suggested that Zfrp8 functions in the formation of mRNP (mRNA ribonucleoprotein complexes and also controls RNA of select Transposable Elements (TEs. Here we show that in Zfrp8/PDCD2 knock down (KD ovaries, specific mRNAs and TE transcripts show increased nuclear accumulation. We also show that Zfrp8/PDCD2 interacts with the (40S small ribosomal subunit through direct interaction with RpS2 (uS5. By studying the distribution of endogenous and transgenic fluorescently tagged ribosomal proteins we demonstrate that Zfrp8/PDCD2 regulates the cytoplasmic levels of components of the small (40S ribosomal subunit, but does not control nuclear/nucleolar localization of ribosomal proteins. Our results suggest that Zfrp8/PDCD2 functions at late stages of ribosome assembly and may regulate the binding of specific mRNA-RNPs to the small ribosomal subunit ultimately controlling their cytoplasmic localization and translation.

  20. Discrimination between ovine Babesia and Theileria species in China based on the ribosomal protein S8 (RPS8) gene.

    Tian, Zhancheng; Liu, Guangyuan; Yin, Hong; Luo, Jianxun; Guan, Guiquan; Luo, Jin; Xie, Junren; Zheng, Jinfeng; Yuan, Xiaosong; Wang, Fangfang; Shen, Hui; Tian, Meiyuan


    Ovine babesiosis and theileriosis are important hemoprotozoal diseases of sheep and goats in tropical and subtropical regions that lead to economic losses in these animals. PCR-restriction fragment length polymorphism (PCR-RFLP) is a reliable molecular diagnostic tool for discriminating Theileria or Babesia species in the same host. In this study, the DNA sequences of a ribosomal protein S8 (RPS8) gene from four species of piroplasms in China were used to develop a species-specific PCR-RFLP diagnostic tool. The sensitivity of the PCR assays was 0.1 pg DNA for B. motasi and 1 pg DNA for T. uilenbergi and 10 pg DNA for Babesia sp. Xinjiang-2005 and T. luwenshuni. The clear size difference of the PCR products allowed for a direct discrimination for B. motasi, Babesia sp. Xinjiang-2005 and ovine Theileria species (T. uilenbergi and T. luwenshuni), except that the mixed infection between T. uilenbergi and T. luwenshuni may be difficult to distinguish, simply after the electrophoretic separation of the amplification products. Further T. uilenbergi and T. luwenshuni diagnoses were made by digesting the PCR product with SacI. The established method could be applicable for the survey of parasite dynamics, and epidemiological studies as well as prevention and control of the disease.

  1. Finite Element Vibration and Dynamic Response Analysis of Engineering Structures

    Jaroslav Mackerle


    Full Text Available This bibliography lists references to papers, conference proceedings, and theses/dissertations dealing with finite element vibration and dynamic response analysis of engineering structures that were published from 1994 to 1998. It contains 539 citations. The following types of structures are included: basic structural systems; ground structures; ocean and coastal structures; mobile structures; and containment structures.

  2. Academic Work, Working Conditions and Job Satisfaction (CPP RPS 48/2013)


    In this analysis of changing academic work, working conditions and job satisfaction in Europe, we will present the academics’ assessment of facilities, resources, and personnel. Subsequently, an overview will be provided about the academic workloads and allocation of time between the four major types of academic activities: teaching, research, service, and administration. A further section will discuss job satisfaction and, related to it, the academics’ income. The chapter provides a gener...

  3. BASE Flexible Array Preliminary Lithospheric Structure Analysis

    Yeck, W. L.; Sheehan, A. F.; Anderson, M. L.; Siddoway, C. S.; Erslev, E.; Harder, S. H.; Miller, K. C.


    The Bighorns Arch Seismic Experiment (BASE) is a Flexible Array experiment integrated with EarthScope. The goal of BASE is to develop a better understanding of how basement-involved foreland arches form and what their link is to plate tectonic processes. To achieve this goal, the crustal structure under the Bighorn Mountain range, Bighorn Basin, and Powder River Basin of northern Wyoming and southern Montana are investigated through the deployment of 35 broadband seismometers, 200 short period seismometers, 1600 “Texan” instruments using active sources and 800 “Texan” instruments monitoring passive sources, together with field structural analysis of brittle structures. The novel combination of these approaches and anticipated simultaneous data inversion will give a detailed structural crustal image of the Bighorn region at all levels of the crust. Four models have been proposed for the formation of the Bighorn foreland arch: subhorizontal detachment within the crust, lithospheric buckling, pure shear lithospheric thickening, and fault blocks defined by lithosphere-penetrating thrust faults. During the summer of 2009, we deployed 35 broadband instruments, which have already recorded several magnitude 7+ teleseismic events. Through P wave receiver function analysis of these 35 stations folded in with many EarthScope Transportable Array stations in the region, we present a preliminary map of the Mohorovicic discontinuity. This crustal map is our first test of how the unique Moho geometries predicted by the four hypothesized models of basement involved arches fit seismic observations for the Bighorn Mountains. In addition, shear-wave splitting analysis for our first few recorded teleseisms helps us determine if strong lithospheric deformation is preserved under the range. These analyses help lead us to our final goal, a complete 4D (3D spatial plus temporal) lithospheric-scale model of arch formation which will advance our understanding of the mechanisms

  4. Structural Analysis of Sandwich Foam Panels

    Kosny, Jan [ORNL; Huo, X. Sharon [Tennessee Technological University


    The Sandwich Panel Technologies including Structural Insulated Panels (SIPs) can be used to replace the conventional wooden-frame construction method. The main purpose of this Cooperative Research and Development Agreement (CRADA) between UT-Battelle, LLC and SGI Venture, Inc. was to design a novel high R-value type of metal sandwich panelized technology. This CRADA project report presents design concept discussion and numerical analysis results from thermal performance study of this new building envelope system. The main objective of this work was to develop a basic concept of a new generation of wall panel technologies which will have R-value over R-20 will use thermal mass to improve energy performance in cooling dominated climates and will be 100% termite resistant. The main advantages of using sandwich panels are as follows: (1) better energy saving structural panels with high and uniform overall wall R-value across the elevation that could not be achieved in traditional walls; and (2) reducing the use of raw materials or need for virgin lumber. For better utilization of these Sandwich panels, engineers need to have a thorough understanding of the actual performance of the panels and system. Detailed analysis and study on the capacities and deformation of individual panels and its assembly have to be performed to achieve that goal. The major project activity was to conduct structural analysis of the stresses, strains, load capacities, and deformations of individual sandwich components under various load cases. The analysis simulated the actual loading conditions of the regular residential building and used actual material properties of the steel facings and foam.

  5. Molecular Eigensolution Symmetry Analysis and Fine Structure

    William G. Harter


    Full Text Available Spectra of high-symmetry molecules contain fine and superfine level cluster structure related to J-tunneling between hills and valleys on rovibronic energy surfaces (RES. Such graphic visualizations help disentangle multi-level dynamics, selection rules, and state mixing effects including widespread violation of nuclear spin symmetry species. A review of RES analysis compares it to that of potential energy surfaces (PES used in Born-Oppenheimer approximations. Both take advantage of adiabatic coupling in order to visualize Hamiltonian eigensolutions. RES of symmetric and D2 asymmetric top rank-2-tensor Hamiltonians are compared with Oh spherical top rank-4-tensor fine-structure clusters of 6-fold and 8-fold tunneling multiplets. Then extreme 12-fold and 24-fold multiplets are analyzed by RES plots of higher rank tensor Hamiltonians. Such extreme clustering is rare in fundamental bands but prevalent in hot bands, and analysis of its superfine structure requires more efficient labeling and a more powerful group theory. This is introduced using elementary examples involving two groups of order-6 (C6 and D3~C3v, then applied to families of Oh clusters in SF6 spectra and to extreme clusters.

  6. Structural dynamic analysis of composite beams

    Suresh, J. K.; Venkatesan, C.; Ramamurti, V.


    In the treatment of the structural dynamic problem of composite materials, two alternate types of formulations, based on the elastic modulus and compliance quantities, exist in the literature. The definitions of the various rigidities are observed to differ in these two approaches. Following these two types of formulation, the structural dynamic characteristics of a composite beam are analyzed. The results of the analysis are compared with those available in the literature. Based on the comparison, the influence of the warping function in defining the coupling terms in the modulus approach and also on the natural frequencies of the beam has been identified. It is found from the analysis that, in certain cases, the difference between the results of the two approaches is appreciable. These differences may be attributed to the constraints imposed on the deformation and flexibility of the beam by the choice of the description of the warping behaviour. Finally, the influence of material properties on the structural dynamic characteristics of the beam is studied for different composites for various angles of orthotropy.

  7. Nonlinear frequency response analysis of structural vibrations

    Weeger, Oliver; Wever, Utz; Simeon, Bernd


    In this paper we present a method for nonlinear frequency response analysis of mechanical vibrations of 3-dimensional solid structures. For computing nonlinear frequency response to periodic excitations, we employ the well-established harmonic balance method. A fundamental aspect for allowing a large-scale application of the method is model order reduction of the discretized equation of motion. Therefore we propose the utilization of a modal projection method enhanced with modal derivatives, providing second-order information. For an efficient spatial discretization of continuum mechanics nonlinear partial differential equations, including large deformations and hyperelastic material laws, we employ the concept of isogeometric analysis. Isogeometric finite element methods have already been shown to possess advantages over classical finite element discretizations in terms of higher accuracy of numerical approximations in the fields of linear vibration and static large deformation analysis. With several computational examples, we demonstrate the applicability and accuracy of the modal derivative reduction method for nonlinear static computations and vibration analysis. Thus, the presented method opens a promising perspective on application of nonlinear frequency analysis to large-scale industrial problems.


    LI Hong-shuang; L(U) Zhen-zhou; YUE Zhu-feng


    Support vector machine (SVM) was introduced to analyze the reliability of the implicit performance function, which is difficult to implement by the classical methods such as the first order reliability method (FORM) and the Monte Carlo simulation (MCS). As a classification method where the underlying structural risk minimization inference rule is employed, SVM possesses excellent learning capacity with a small amount of information and good capability of generalization over the complete data. Hence,two approaches, i.e., SVM-based FORM and SVM-based MCS, were presented for the structural reliability analysis of the implicit limit state function. Compared to the conventional response surface method (RSM) and the artificial neural network (ANN), which are widely used to replace the implicit state function for alleviating the computation cost,the more important advantages of SVM are that it can approximate the implicit function with higher precision and better generalization under the small amount of information and avoid the "curse of dimensionality". The SVM-based reliability approaches can approximate the actual performance function over the complete sampling data with the decreased number of the implicit performance function analysis (usually finite element analysis), and the computational precision can satisfy the engineering requirement, which are demonstrated by illustrations.

  9. Fiber structural analysis by synchrotron radiation

    Kojima, J I; Kikutani, T


    Topics of fiber structural analysis by synchrotron radiation are explained. There are only three synchrotron radiation facilities in the world, SPring-8 (Super Photon ring-8) in Japan, APS (Advanced Photon Source) in U.S.A. and ESRF (European Synchrotron Radiation Facility) in France. Online measurement of melt spinning process of PET and Nylon6 is explained in detail. Polypropylene and PBO (poly-p-phenylenebenzobisoxazole) was measured by WAXD (Wide Angle X-ray Diffraction)/SAXS (Small Angle X-ray Scattering) at the same time. Some examples of measure of drawing process of fiber are described. The structure formation process of spider's thread was measured. Micro beam of X-ray of synchrotron facility was improved and it attained to 65nm small angle resolving power by 10 mu m beamsize. (S.Y.)

  10. MicroRNA-223-3p inhibits the angiogenesis of ischemic myocardial microvascular endothelial cells via modulating Rps6kb1/HIF-1α signal pathway%微小RNA-223-3p通过调节Rps6kb1/HIF-1α信号通路抑制缺血心肌微血管内皮细胞血管新生

    戴国华; 宋宪波; 马培泽; 刘宁; 姚静


    Objective To explore the role of microRNA on the myocardial microvascular endothelial cells (CMECs) of ischemic heart rats in the process of angiogenesis and related regulation mechanism.Methods Myocardial ischemic rats model was established by coronary ligation.Seven days after operation,the ischemic CMECs were cultured by the method of planting myocardium tissue and identified by immunocytochemistry to observe the biological characteristics of ischemic CMECs angiogenesis,to determine the window period of migration,proliferation,tube formation in the process of its angiogenesis.Dynamic expression changes of microRNA in the process of ischemic CMECs angiogenesis was detected using microRNA chip and further verified by real-time PCR,the core microRNA of the ischemic CMECs was defined and the predicted target genes of core microRNA were determined by bioinformatics methods and real-time PCR.At the same time,the protein expression of target gene and angiogenesis related genes of p38MAPK,PI3K,Akt,VEGF were measured by Western blot.Results The CMECs of rats presented typical characteristics of microvascular endothelial cells,and factor Ⅷ,CD3 1 related antigens were all positively stained by immunocytochemical analysis.The migration window period was on the first day,and the tube formation window period was on the second day of both control and ischemic groups,while the proliferation window period was on the third day for the normal group,and the sixth day for ischemic group.According to the expressional difference and their relationship with angiogenesis,miRNA-223-3p was ultimately determined as the core microRNA in the process of ischemic CMECs angiogenesis,real-time PCR verified this hypothesis.Bioinformatics methods predicted that Rps6kb1 is the target genes of miRNA-223-3p,the pathway analysis showed that Rps6kb1 could regulate angiogenesis via HIF-1α signal pathway.Moreover,the mRNA and protein expression of VEGF,p38MAPK,PI3K,Akt,which were the downstream

  11. Resistance to Selumetinib (AZD6244 in Colorectal Cancer Cell Lines is Mediated by p70S6K and RPS6 Activation

    Silvina Grasso


    Full Text Available Selumetinib (AZD6244, ARRY-142886 is a MEK1/2 inhibitor that has gained interest as an anti-tumour agent. We have determined the degree of sensitivity/resistance to Selumetinib in a panel of colorectal cancer cell lines using cell proliferation and soft agar assays. Sensitive cell lines underwent G1 arrest, whereas Selumetinib had no effect on the cell cycle of resistant cells. Some of the resistant cell lines showed high levels of ERK1/2 phosphorylation in the absence of serum. Selumetinib inhibited phosphorylation of ERK1/2 and RSK and had no effect on AKT phosphorylation in both sensitive and resistant cells. Furthermore, mutations in KRAS, BRAF, or PIK3CA were not clearly associated with Selumetinib resistance. Surprisingly, Selumetinib was able to inhibit phosphorylation of p70 S6 kinase (p70S6K and its downstream target ribosomal protein S6 (RPS6 in sensitive cell lines. However, p70S6K and RPS6 phosphorylation remained unaffected or even increased in resistant cells. Moreover, in some of the resistant cell lines p70S6K and RPS6 were phosphorylated in the absence of serum. Interestingly, colorectal primary cultures derived from tumours excised to patients exhibited the same behaviour than established cell lines. Pharmacological inhibition of p70S6K using the PI3K/mTOR inhibitor NVP-BEZ235, the specific mTOR inhibitor Rapamycin and the specific p70S6K inhibitor PF-4708671 potentiated Selumetinib effects in resistant cells. In addition, biological inhibition of p70S6K using siRNA rendered responsiveness to Selumetinib in resistant cell lines. Furthermore, combination of p70S6K silencing and PF-47086714 was even more effective. We can conclude that p70S6K and its downstream target RPS6 are potential biomarkers of resistance to Selumetinib in colorectal cancer.

  12. The transcription factor Ace2 and its paralog Swi5 regulate ethanol production during static fermentation through their targets Cts1 and Rps4a in Saccharomyces cerevisiae.

    Wu, Yao; Du, Jie; Xu, Guoqiang; Jiang, Linghuo


    Saccharomyces cerevisiae is the most widely used fermentation organism for ethanol production. However, the gene expression regulatory networks behind the ethanol fermentation are still not fully understood. Using a static fermentation model, we examined the ethanol yields on biomass of deletion mutants for 77 yeast genes encoding nonessential transcription factors, and found that deletion mutants for ACE2 and SWI5 showed dramatically increased ethanol yields. Overexpression of ACE2 or SWI5 in wild type cells reduced their ethanol yields. Furthermore, among the 34 target genes regulated by Ace2 and Swi5, deletion of CTS1,RPS4a,SIC1,EGT2,DSE2, or SCP160 led to increased ethanol yields, with the former two showing higher effects. Overexpression of CTS1 or RPS4a in both ace2/ace2 and swi5/swi5 mutants reduced their ethanol yields. In contrast, deletion of MCR1 or HO significantly decreased ethanol yields, with the former one showing the highest effect. Therefore, Ace2 and Swi5 are two negative regulators of ethanol yield during static fermentation of yeast cells, and both CTS1 and RPS4a are major effectors mediating these two transcription factors in regulating ethanol production.

  13. Chromosomal instability and telomere shortening in long-term culture of hematopoietic stem cells: insights from a cell culture model of RPS14 haploinsufficiency.

    Thomay, K; Schienke, A; Vajen, B; Modlich, U; Schambach, A; Hofmann, W; Schlegelberger, B; Göhring, G


    The fate of cultivated primary hematopoietic stem cells (HSCs) with respect to genetic instability and telomere attrition has not yet been described in great detail. Thus, knowledge of the genetic constitution of HSCs is important when interpreting results of HSCs in culture. While establishing a cell culture model for myelodysplastic syndrome with a deletion in 5q by performing RPS14 knockdown, we found surprising data that may be of importance for any CD34+ cell culture experiments. We performed cytogenetic analyses and telomere length measurement on transduced CD34+ cells and untransduced control cells to observe the effects of long-term culturing. Initially, CD34+ cells had a normal median telomere length of about 12 kb and showed no signs of chromosomal instability. During follow-up, the median telomere length seemed to decrease and, simultaneously, increased chromosomal instability could be observed - in modified and control cells. One culture showed a clonal monosomy 7 - independent of prior RPS14 knockdown. During further culturing, it seemed that the telomeres re-elongated, and chromosomes stabilized, while TERT expression was not elevated. In summary, irrespective of our results of RPS14 knockdown in the long-term culture of CD34+ cells, it becomes clear that cell culture artefacts inducing telomere shortening and chromosomal instability have to be taken into account and regular cytogenetic analyses should always be performed.

  14. Probabilistic analysis of linear elastic cracked structures


    This paper presents a probabilistic methodology for linear fracture mechanics analysis of cracked structures. The main focus is on probabilistic aspect related to the nature of crack in material. The methodology involves finite element analysis; statistical models for uncertainty in material properties, crack size, fracture toughness and loads; and standard reliability methods for evaluating probabilistic characteristics of linear elastic fracture parameter. The uncertainty in the crack size can have a significant effect on the probability of failure, particularly when the crack size has a large coefficient of variation. Numerical example is presented to show that probabilistic methodology based on Monte Carlo simulation provides accurate estimates of failure probability for use in linear elastic fracture mechanics.

  15. Rhetorical structure theory and text analysis

    Mann, William C.; Matthiessen, Christian M. I. M.; Thompson, Sandra A.


    Recent research on text generation has shown that there is a need for stronger linguistic theories that tell in detail how texts communicate. The prevailing theories are very difficult to compare, and it is also very difficult to see how they might be combined into stronger theories. To make comparison and combination a bit more approachable, we have created a book which is designed to encourage comparison. A dozen different authors or teams, all experienced in discourse research, are given exactly the same text to analyze. The text is an appeal for money by a lobbying organization in Washington, DC. It informs, stimulates and manipulates the reader in a fascinating way. The joint analysis is far more insightful than any one team's analysis alone. This paper is our contribution to the book. Rhetorical Structure Theory (RST), the focus of this paper, is a way to account for the functional potential of text, its capacity to achieve the purposes of speakers and produce effects in hearers. It also shows a way to distinguish coherent texts from incoherent ones, and identifies consequences of text structure.

  16. Aberrant methylation of the X-linked ribosomal S6 kinase RPS6KA6 (RSK4) in endometrial cancers.

    Dewdney, Summer B; Rimel, B J; Thaker, Premal H; Thompson, Dominic M; Schmidt, Amy; Huettner, Phyllis; Mutch, David G; Gao, Feng; Goodfellow, Paul J


    Effective treatments for advanced endometrial cancer are lacking. Novel therapies that target specific pathways hold promise for better treatment outcomes with less toxicity. Mutation activation of the FGFR2/RAS/ERK pathway is important in endometrial tumorigenesis. RPS6KA6 (RSK4) is a putative tumor suppressor gene and is a target of the ERK signaling pathway. We explored the role of RSK4 in endometrial cancer. We showed that RSK4 is expressed in normal endometrial tissue and is absent or much reduced in endometrial cancer. On the basis of previous reports on methylation in other cancers, we hypothesized that the absence of RSK4 transcript is associated with epigenetic silencing rather than mutation. We determined the methylation and expression status of RSK4 in primary endometrial cancers and cell lines and the effects of treatment with a demethylating agent. The relationship between RSK4 methylation and clinicopathologic features was assessed. RSK4 is frequently hypermethylated in endometrial cancer cells lines and in primary endometrial cancer compared with normal endometrial tissue. RSK4 methylation was significantly associated with tumor grade, with higher grade tumors having lower levels of methylation (P = 0.03). RSK4 methylation levels were not associated with other clinical variables. We did find that RSK4 methylation was significantly correlated with expression in primary endometrial tumors and in cell lines. Reactivation of RSK4 by 5-azacytidine was successfully performed showing 8- to more than 1,200-fold increases in transcript levels. RSK4 appears to be epigenetically silenced in endometrial cancer as evidenced by hypermethylation. Its role as a suppressor in endometrial cancer, however, remains uncertain. ©2011 AACR.

  17. A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker.

    Yu-Kuo Tsai

    Full Text Available The streptomycin counter-selection system is a useful tool for constructing unmarked in-frame gene deletions, which is a fundamental approach to study bacteria and their pathogenicity at the molecular level. A prerequisite for this system is acquiring a streptomycin-resistant strain due to rpsL mutations, which encodes the ribosomal protein S12. However, in this study no streptomycin resistance was found to be caused by rpsL mutations in all 127 clinical strains of Klebsiella pneumoniae isolated from liver abscess patients. By screening 107 spontaneous mutants of streptomycin resistance from a clinical strain of K. pneumoniae, nucleotide substitution or insertion located within the rpsL was detected in each of these strains. Thirteen different mutants with varied S12 proteins were obtained, including nine streptomycin-dependent mutants. The virulence of all four streptomycin-resistant mutants was further evaluated. Compared with the parental strain, the K42N, K42T and K87R mutants showed a reduction in growth rate, and the K42N and K42T mutants became susceptible to normal human serum. In the mice LD50 (the bacterial dose that caused 50% death assay, the K42N and K42T mutants were ∼ 1,000-fold less lethal (∼ 2 × 10(5 CFU and the K87R mutant was ∼ 50-fold less lethal (∼ 1 × 10(4 CFU than the parental strain (∼ 2 × 10(2 CFU. A K42R mutant showed non-observable effects on the above assays, while this mutant exhibited a small cost (P < 0.01 in an in vitro growth competition experiment. In summary, most of the K. pneumoniae strains with streptomycin resistance caused by rpsL mutations are less virulent than their parental strain in the absence of streptomycin. The K42R mutant showed similar pathogenicity to its parental strain and should be one of the best choices when using rpsL as a counter-selection marker.

  18. A disputed evidence on obesity: comparison of the effects of Rcan2 −/− and Rps6kb1 −/− mutations on growth and body weight in C57BL/6J mice* #

    Zhao, Jing; Li, Shi-wei; Gong, Qian-qian; Ding, Ling-cui; Jin, Ye-cheng; Zhang, Jian; Gao, Jian-gang; Sun, Xiao-yang


    It is widely accepted that body weight and adipose mass are tightly regulated by homeostatic mechanisms, in which leptin plays a critical role through hypothalamic pathways, and obesity is a result of homeostatic disorder. However, in C57BL/6J mice, we found that Rcan2 increases food intake and plays an important role in the development of age-and diet-induced obesity through a leptin-independent mechanism. RCAN2 was initially identified as a thyroid hormone (T3)-responsive gene in human fibroblasts. Expression of RCAN2 is regulated by T3 through the PI3K-Akt/PKB-mTOR-Rps6kb1 signaling pathway. Intriguingly, both Rcan2 −/− and Rps6kb1 −/− mutations were reported to result in lean phenotypes in mice. In this study we compared the effects of these two mutations on growth and body weight in C57BL/6J mice. We observed reduced body weight and lower fat mass in both Rcan2 −/− and Rps6kb1 −/− mice compared to the wild-type mice, and we reported other differences unique to either the Rcan2 −/− or Rps6kb1 −/− mice. Firstly, loss of Rcan2 does not directly alter body length; however, Rcan2 −/− mice exhibit reduced food intake. In contrast, Rps6kb1 −/− mice exhibit abnormal embryonic development, which leads to smaller body size and reduced food intake in adulthood. Secondly, when fed a normal chow diet, Rcan2 −/− mice weigh significantly more than Rps6kb1 −/− mice, but both Rcan2 −/− and Rps6kb1−/− mice develop similar amounts of epididymal fat. On a high-fat diet, Rcan2 −/− mice gain body weight and fat mass at slower rates than Rps6kb1 −/− mice. Finally, using the double-knockout mice (Rcan2 −/− Rps6kb1 −/−), we demonstrate that concurrent loss of Rcan2 and Rps6kb1 has an additive effect on body weight reduction in C57BL/6J mice. Our data suggest that Rcan2 and Rps6kb1 mutations both affect growth and body weight of mice, though likely through different mechanisms. PMID:27604858


    HanJinyan; YuZhiwei


    A surface spline function is used to fit a coal seam surface in structural analysis in coal geology. From the surface spline function, the first and second partial derivatives can also be derived and used to structural analysis, especially for recognition of the concealed structures. The detection of structures related to faulting is emphasized.

  20. RPS Micro Diskette

    Small flexible plastic disk covered with a magnetic substance used to record data and computer programs. They can normally contain 1.44 MB of data and they are convenient for moving small amounts of data as they are transportable.

  1. Structured analysis and modeling of complex systems

    Strome, David R.; Dalrymple, Mathieu A.


    The Aircrew Evaluation Sustained Operations Performance (AESOP) facility at Brooks AFB, Texas, combines the realism of an operational environment with the control of a research laboratory. In recent studies we collected extensive data from the Airborne Warning and Control Systems (AWACS) Weapons Directors subjected to high and low workload Defensive Counter Air Scenarios. A critical and complex task in this environment involves committing a friendly fighter against a hostile fighter. Structured Analysis and Design techniques and computer modeling systems were applied to this task as tools for analyzing subject performance and workload. This technology is being transferred to the Man-Systems Division of NASA Johnson Space Center for application to complex mission related tasks, such as manipulating the Shuttle grappler arm.

  2. Purification and Structural Analysis of Desmoplakin.

    Choi, Hee-Jung; Weis, William I


    Desmoplakin (DP) is an obligate component of desmosomes, where it links the desmosomal cadherin/plakoglobin/plakophilin assembly to intermediate filaments. DP contains a large amino-terminal domain (DPNT) that binds to the cadherin/plakoglobin/plakophilin complex, a central coiled-coil domain that dimerizes the molecule, and a C-terminal domain (DPCT) that binds to intermediate filaments. DPNT contains a plakin domain, comprising a set of spectrin-like repeats. DPCT contains three plakin repeat domains, each formed by 4.5 repeats of a sequence motif known as a plakin repeat that bind to intermediate filaments. Here, we review purification, biochemical characterization, and structural analysis of the DPNT plakin domain and the DPCT plakin repeat domains.


    LI Jinghua


    Over the past two decades,structural decomposition analysis(SDA)has developed into a major analytical tool in the field of input-output(IO)techniques,but the method was found to suffer from one or more of the following problems.The decomposition forms,which are used to measure the contribution of a specific determinant,are not unique due to the existence of a multitude of equivalent forms,irrational due to the weights of different determinants not matching,inexact due to the existence of large interaction terms.In this paper,a decomposition method is derived to overcome these deficiencies,and we prove that the result of this approach is equal to the Shapley value in cooperative games,and so some properties of the method are obtained.Beyond that,the two approaches that have been used predominantly in the literature have been proved to be the approximate solutions of the method.

  4. Decision variables analysis for structured modeling

    潘启树; 赫东波; 张洁; 胡运权


    Structured modeling is the most commonly used modeling method, but it is not quite addaptive to significant changes in environmental conditions. Therefore, Decision Variables Analysis(DVA), a new modelling method is proposed to deal with linear programming modeling and changing environments. In variant linear programming , the most complicated relationships are those among decision variables. DVA classifies the decision variables into different levels using different index sets, and divides a model into different elements so that any change can only have its effect on part of the whole model. DVA takes into consideration the complicated relationships among decision variables at different levels, and can therefore sucessfully solve any modeling problem in dramatically changing environments.

  5. Inelastic analysis of solids and structures

    Kojic, M; Bathe, K J; Koji?, Milo


    Inelastic Analysis of Solids and Structures presents in a unified manner the physical and theoretical background of inelastic material models and computational methods, and illustrates the behavior of the models in typical engineering conditions.It is based on experimental observations and principles of mechanics, thus describing computational algorithms for stress calculation and presenting solved examples.The theoretical background is given to an extent necessary to describe the commonly employed material models in metal isotropic and orthotropic plasticity, thermoplasticity and viscoplasticity, and the plasticity of geological materials.The computational algorithms are developed in a unified manner with some detailed derivations of the algorithmic relations.The solved examples are designed to give insight into the material behavior in various engineering conditions, and to demonstrate the application of the computational algorithms.

  6. Isogeometric analysis in electronic structure calculations

    Cimrman, Robert; Kolman, Radek; Tůma, Miroslav; Vackář, Jiří


    In electronic structure calculations, various material properties can be obtained by means of computing the total energy of a system as well as derivatives of the total energy w.r.t. atomic positions. The derivatives, also known as Hellman-Feynman forces, require, because of practical computational reasons, the discretized charge density and wave functions having continuous second derivatives in the whole solution domain. We describe an application of isogeometric analysis (IGA), a spline modification of finite element method (FEM), to achieve the required continuity. The novelty of our approach is in employing the technique of B\\'ezier extraction to add the IGA capabilities to our FEM based code for ab-initio calculations of electronic states of non-periodic systems within the density-functional framework, built upon the open source finite element package SfePy. We compare FEM and IGA in benchmark problems and several numerical results are presented.

  7. Power System Overview for the Small RPS Centaur Flyby and the Mars Polar Hard Lander NASA COMPASS Studies

    Cataldo, Robert L.


    The NASA Glenn Research Center (GRC) Radioisotope Power System Program Office (RPSPO) sponsored two studies lead by their mission analysis team. The studies were performed by NASA GRCs Collaborative Modeling for Parametric Assessment of Space Systems (COMPASS) team. Typically a complete toplevel design reference mission (DRM) is performed assessing conceptual spacecraft design, launch mass, trajectory, science strategy and sub-system design such as, power, propulsion, structure and thermal.

  8. Structural analysis of nucleosomal barrier to transcription.

    Gaykalova, Daria A; Kulaeva, Olga I; Volokh, Olesya; Shaytan, Alexey K; Hsieh, Fu-Kai; Kirpichnikov, Mikhail P; Sokolova, Olga S; Studitsky, Vasily M


    Thousands of human and Drosophila genes are regulated at the level of transcript elongation and nucleosomes are likely targets for this regulation. However, the molecular mechanisms of formation of the nucleosomal barrier to transcribing RNA polymerase II (Pol II) and nucleosome survival during/after transcription remain unknown. Here we show that both DNA-histone interactions and Pol II backtracking contribute to formation of the barrier and that nucleosome survival during transcription likely occurs through allosterically stabilized histone-histone interactions. Structural analysis indicates that after Pol II encounters the barrier, the enzyme backtracks and nucleosomal DNA recoils on the octamer, locking Pol II in the arrested state. DNA is displaced from one of the H2A/H2B dimers that remains associated with the octamer. The data reveal the importance of intranucleosomal DNA-protein and protein-protein interactions during conformational changes in the nucleosome structure on transcription. Mechanisms of nucleosomal barrier formation and nucleosome survival during transcription are proposed.

  9. Steerable wavelet analysis of CMB structures alignment

    Vielva, P; Martínez-González, E; Vandergheynst, P


    This paper reviews the application of a novel methodology for analysing the isotropy of the universe by probing the alignment of local structures in the CMB. The strength of the proposed methodology relies on the steerable wavelet filtering of the CMB signal. One the one hand, the filter steerability renders the computation of the local orientation of the CMB features affordable in terms of computation time. On the other hand, the scale-space nature of the wavelet filtering allows to explore the alignment of the local structures at different scales, probing possible different phenomena. We present the WMAP first-year data analysis recently performed by the same authors (Wiaux et al.), where an extremely significant anisotropy was found. In particular, a preferred plane was detected, having a normal direction with a northern end position close to the northern end of the CMB dipole axis. In addition, a most preferred direction was found in that plane, with a northern end direction very close to the north eclipt...

  10. Microbial Community Structure of Activated Sludge for Biosolubilization of Two Different Rock Phosphates.

    Xiao, Chunqiao; Wu, Xiaoyan; Liu, Tingting; Xu, Guang; Chi, Ruan


    A microbial consortium was directly taken from activated sludge and was used to solubilize rock phosphates (RPs) in a lab-scale bioreactor in this study. Results showed that the microbial consortium could efficiently release soluble phosphorus (P) from the RPs, and during 30-day incubation, it grew well in the bioreactor and reduced the pH of the solutions. The biosolubilization process was also illustrated by the observation of scanning electron microscopy combined with an energy dispersive X-ray spectroscopy (SEM-EDX), which showed an obvious corrosion on the ore surfaces, and most elements were removed from the ore samples. The analysis of microbial community structure by Illumina 16S ribosomal RNA (rRNA) gene and 18S rRNA gene MiSeq sequencing reflected different microbial diversity and richness in the solutions added with different ore samples. A lower richness and diversity of bacteria but a higher richness and diversity of fungi occurred in the solution added with ore sample 1 compared to that of in the solution added with ore sample 2. Alphaproteobacteria and Saccharomycetes were the dominating bacterial and fungal group, respectively, both in the solutions added with ore samples 1 and 2 at the class level. However, their abundances in the solution added with ore sample 1 were obviously lower than that in the solution added with ore sample 2. This study provides new insights into our understanding of the microbial community structure in the biosolubilization of RPs by a microbial consortium directly taken from activated sludge.

  11. Empirical analysis between industrial structure and economic growth of china

    姚西龙; 尤津; 郝鹏飞


    In recent years,the relationship between the industrial structure and economic growth is more and more concerned by scholars. According to the theory of industrial structure and economic development, this article use regression analysis method that estimates the three major industries’ contribute to Chinese economic growth and use cluster analysis methods, then discuss how to optimize the indus-trial structure.

  12. Identification of nucleosome assembly protein 1 (NAP1) as an interacting partner of plant ribosomal protein S6 (RPS6) and a positive regulator of rDNA transcription

    Son, Ora [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Kim, Sunghan [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921 (Korea, Republic of); Shin, Yun-jeong [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Kim, Woo-Young [College of Pharmacy, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Koh, Hee-Jong, E-mail: [Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921 (Korea, Republic of); Cheon, Choong-Ill, E-mail: [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of)


    The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. In our previous study, we identified a novel role of plant RPS6, which negatively regulates rDNA transcription, forming a complex with a plant-specific histone deacetylase, AtHD2B. Here we report that the Arabidopsis RPS6 interacts additionally with a histone chaperone, nucleosome assembly protein 1(AtNAP1;1). The interaction does not appear to preclude the association of RPS6 with AtHD2B, as the AtNAP1 was also able to interact with AtHD2B as well as with an RPS6-AtHD2B fusion protein in the BiFC assay and pulldown experiment. Similar to a positive effect of the ribosomal S6 kinase 1 (AtS6K1) on rDNA transcription observed in this study, overexpression or down regulation of the AtNAP1;1 resulted in concomitant increase and decrease, respectively, in rDNA transcription suggesting a positive regulatory role played by AtNAP1 in plant rDNA transcription, possibly through derepression of the negative effect of the RPS6-AtHD2B complex. - Highlights: • Nucleosome assembly protein 1 (AtNAP1) interacts with RPS6 as well as with AtHD2B. • rDNA transcription is regulated S6K1. • Overexpression or down regulation of AtNAP1 results in concomitant increase or decrease in rDNA transcription.

  13. Error Analysis for RADAR Neighbor Matching Localization in Linear Logarithmic Strength Varying Wi-Fi Environment

    Zhou, Mu; Tian, Zengshan; Xu, Kunjie; Yu, Xiang; Wu, Haibo


    ...) in logarithmic received signal strength (RSS) varying Wi-Fi environment. To the best of our knowledge, little comprehensive analysis work has appeared on the error performance of neighbor matching localization with respect to the deployment of RPs...

  14. Analysis of latent structures in linear systems

    Høskuldsson, Agnar


    In chemometrics the emphasis is on latent structure models. The latent structure is the part of the data that the modeling task is based upon. This paper is addressing some fundamental issues, when latent structures are used. The paper consists of three parts. The first part is concerned defining...

  15. Structural Response Analysis under Dependent Variables Based on Probability Boxes

    Xiao, Z; Yang, G


      This paper considers structural response analysis when structural uncertainty parameters distribution cannot be specified precisely due to lack of information and there are complex dependencies in the variables...

  16. Development of numerical procedures for analysis of complex structures

    Gupta, K. K.


    The paper is concerned with the development of novel numerical procedures for the solution of static, stability, free vibration and dynamic response analysis of large, complex practical structures. Thus, details of numerical algorithms evolved for dynamic analysis of usual non-rotating and also rotating structures as well as finite dynamic elements are presented in the paper. Furthermore, the article provides some description of a general-purpose computer program STARS specifically developed for efficient analysis of complex practical structures.

  17. Analysis of slender structures using mechanics of structure genome and open source codes

    Liu, Xin

    Structural genome (SG) is the smallest mathematical building block that contains all constitutive information of a structure. SG is a bridge connecting the material microstructures and the macroscopic structural analysis, so mechanics of structure genome (MSG) is an approach that unifies micromechanics and structural mechanics. There are three types of SG: 1D SG, 2D SG and 3D SG depending on the heterogeneity of the original structure. Once the SG of a structure is identified, the effective properties for corresponding structures including beams, plates/shells, or 3D solids can be obtained by the homogenization analysis of the SG. These effective properties can be used for the macroscopic structural analysis. After macroscopic structural analysis, the global structural responses can be the input parameters to get the local displacements/stress/strain using dehomogenization analysis of the SG. The mechanics of structure genome is implemented in a general purpose composites software called SwiftComp(TM). Slender structures are widely used in various industries, such as civil, aerospace and structural engineering. Using MSG, a new structural analysis approach is used to study the slender structures. In order to let users easily get the access to the software using this approach, several open source codes have been modified and upgraded. Graphical user interface is built on Gmsh, and CalculiX is chosen as the structural solver. New beam elements have been added to CalculiX to make the better use of the capability of SwiftComp(TM). Both linear and quadratic beam elements for Euler-Bernoulli and Timoshenko beam models are derived. Several numerical models show that the results using MSG and its companion code SwiftComp(TM) agree well with the 3D finite element analysis. The MSG approach greatly simplified the modeling process while maintaining accuracy. Therefore, the MSG approach provides an alternative way to analyze structures, especially when the structures are

  18. Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures

    Jilie KONG; Shaoning YU


    Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its applications to protein structural studies. The experimental skills, data analysis, and correlations between the FTIR spectroscopic bands and protein secondary structure components are discussed. The applications of FTIR to the secondary structure analysis, conformational changes, structural dynamics and stability studies of proteins are also discussed.

  19. Streptomycin affinity depends on 13 amino acids forming a loop in homology modelled ribosomal S12 protein (rpsL gene) of Lysinibacillus sphaericus DSLS5 associated with marine sponge (Tedania anhelans).

    Suriyanarayanan, Balasubramanian; Lakshmi, Praveena Pothuraju; Santhosh, Ramachandran Sarojini; Dhevendaran, Kandasamy; Priya, Balakrishnan; Krishna, Shivaani


    Streptomycin, an antibiotic used against microbial infections, inhibits the protein synthesis by binding to ribosomal protein S12, encoded by rpsL12 gene, and associated mutations cause streptomycin resistance. A streptomycin resistant, Lysinibacillus sphaericus DSLS5 (MIC >300 µg/mL for streptomycin), was isolated from a marine sponge (Tedania anhelans). The characterisation of rpsL12 gene showed a region having similarity to long terminal repeat sequences of murine lukemia virus which added 13 amino acids for loop formation in RpsL12; in addition, a K56R mutation which corresponds to K43R mutation present in streptomycin-resistant Escherichia coli is also present. The RpsL12 protein was modelled and compared with that of Lysinibacillus boronitolerans, Escherichia coli and Mycobacterium tuberculosis. The modelled proteins docked with streptomycin indicate compound had less affinity. The effect of loop on streptomycin resistance was analysed by constructing three different models of RpsL12 by, (i) removing both loop and mutation, (ii) removing the loop alone while retaining the mutation and (iii) without mutation having loop. The results showed that the presence of loop causes streptomycin resistance (decreases the affinity), and it further enhanced in the presence of mutation at 56th codon. Further study will help in understanding the evolution of streptomycin resistance in organisms.

  20. Optimal analysis of structures by concepts of symmetry and regularity

    Kaveh, Ali


    Optimal analysis is defined as an analysis that creates and uses sparse, well-structured and well-conditioned matrices. The focus is on efficient methods for eigensolution of matrices involved in static, dynamic and stability analyses of symmetric and regular structures, or those general structures containing such components. Powerful tools are also developed for configuration processing, which is an important issue in the analysis and design of space structures and finite element models. Different mathematical concepts are combined to make the optimal analysis of structures feasible. Canonical forms from matrix algebra, product graphs from graph theory and symmetry groups from group theory are some of the concepts involved in the variety of efficient methods and algorithms presented. The algorithms elucidated in this book enable analysts to handle large-scale structural systems by lowering their computational cost, thus fulfilling the requirement for faster analysis and design of future complex systems. The ...

  1. Interval Analysis of the Finite Element Method for Stochastic Structures

    刘长虹; 刘筱玲; 陈虬


    A random parameter can be transformed into an interval number in the structural analysis with the concept of the confidence interval. Hence, analyses of uncertain structural systems can be used in the traditional FEM software. In some cases, the amount of solutions in stochastic structures is nearly as many as that in the traditional structural problems. In addition, a new method to evaluate the failure probability of structures is presented for the needs of the modern engineering design.

  2. Translational machinery of the chaetognath Spadella cephaloptera: a transcriptomic approach to the analysis of cytosolic ribosomal protein genes and their expression

    Casanova Jean-Paul


    Full Text Available Abstract Background Chaetognaths, or arrow worms, are small marine, bilaterally symmetrical metazoans. The objective of this study was to analyse ribosomal protein (RP coding sequences from a published collection of expressed sequence tags (ESTs from a chaetognath (Spadella cephaloptera and to use them in phylogenetic studies. Results This analysis has allowed us to determine the complete primary structures of 23 out of 32 RPs from the small ribosomal subunit (SSU and 32 out of 47 RPs from the large ribosomal subunit (LSU. Ten proteins are partially determined and 14 proteins are missing. Phylogenetic analyses of concatenated RPs from six animals (chaetognath, echinoderm, mammalian, insect, mollusc and sponge and one fungal taxa do not resolve the chaetognath phylogenetic position, although each mega-sequence comprises approximately 5,000 amino acid residues. This is probably due to the extremely biased base composition and to the high evolutionary rates in chaetognaths. However, the analysis of chaetognath RP genes revealed three unique features in the animal Kingdom. First, whereas generally in animals one RP appeared to have a single type of mRNA, two or more genes are generally transcribed for one RP type in chaetognath. Second, cDNAs with complete 5'-ends encoding a given protein sequence can be divided in two sub-groups according to a short region in their 5'-ends: two novel and highly conserved elements have been identified (5'-TAATTGAGTAGTTT-3' and 5'-TATTAAGTACTAC-3' which could correspond to different transcription factor binding sites on paralog RP genes. And, third, the overall number of deduced paralogous RPs is very high compared to those published for other animals. Conclusion These results suggest that in chaetognaths the deleterious effects of the presence of paralogous RPs, such as apoptosis or cancer are avoided, and also that in each protein family, some of the members could have tissue-specific and extra-ribosomal functions

  3. Crystal structure analysis of intermetallic compounds

    Conner, R. A., Jr.; Downey, J. W.; Dwight, A. E.


    Study concerns crystal structures and lattice parameters for a number of new intermetallic compounds. Crystal structure data have been collected on equiatomic compounds, formed between an element of the Sc, Ti, V, or Cr group and an element of the Co or Ni group. The data, obtained by conventional methods, are presented in an easily usable tabular form.

  4. Liquefaction mathematical analysis for improvement structures stability

    Azam Khodashenas Pelko


    Full Text Available The stability of any structure is possible if foundation is appropriately designed. The Bandar abbas is the largest and most important port of Iran, with high seismicity and occurring strong earthquakes in this territory, the soil mechanical properties of different parts of city have been selected as the subject of current research. The data relating to the design of foundation for improvement of structure at different layer of subsoil have been collected and, accordingly, soil mechanical properties have been evaluated. The results of laboratory experiments can be used for evaluation of geotechnical characteristics of urban area for development a region with high level of structural stability. Ultimately, a new method for calculation of liquefaction force is suggested. It is applicable for improving geotechnical and structure codes and also for reanalysis of structure stability of previously constructed buildings.

  5. 口腔鳞癌浸润前沿、中心和癌间质TP53、RPS6基因变化的研究%The TP53 and RPS6 alterations at the invasive tumor front, center and stroma of oral squamous cell carcinoma

    王新红; 樊明文; 孙志军; 陈新明; 王丽; 李原


    目的 检测微卫星位点TP53、RPS6在口腔鳞癌浸润前沿、中心及癌间质细胞中的基因变化,揭示口腔鳞癌的生物学行为.方法 运用激光捕获显微切割分别获取同一肿瘤浸润前沿、中心及癌间质足量的细胞,提取DNA,PCR-变性PAGE电泳检测TP53和RPS6位点的基因变化.结果 浸润前沿、中心和癌间质中TP53和RPS6存在杂合性缺失(loss of heterozygosity,LOH)和微卫星不稳定(microsatellite instability,MI),发生率从23.5%(4/17)到64.7%(11/17),且LOH和(或)MI的模式存在不同.上皮TP53和RPS6的LOH和MI发生率分别为70.6%(12/17)和64.7%(11/17),间质为43.8%(7/16)和23.5%(4/17),癌实质较间质发生率高,差异有统计学意义(P<0.05).浸润前沿TP53的LOH发生率为64.7%(11/17),中心为60.0%(9/15).RPS6在浸润前沿的LOH和MI为58.8%(10/17),中心为29.4%(5/17).两位点总发生率分别为62.5%(20/32)和44.1%(15/34),差异有统计学意义(P<0.05).结论 部分口腔鳞癌样本浸润前沿、中心和肿瘤间质的基因变化不同,癌实质基因变化率与肿瘤分化程度有关.

  6. Shielding structure analysis for LSDS facility

    Choi, Hong Yeop; Kim, Jeong Dong; Lee, Yong Deok; Kim, Ho Dong [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)


    The nuclear material (Pyro, Spent nuclear fuel) itself and the target material to generate neutrons is the LSDS system for isotopic fissile assay release of high intensity neutron and gamma rays. This research was performed to shield from various strong radiation. A shielding evaluation was carried out with a facilities model of LSDS system. The MCNPX 2.5 code was used and a shielding evaluation was performed for the shielding structure and location. The radiation dose based on the hole structure and location of the wall was evaluated. The shielding evaluation was performed to satisfy the safety standard for a normal person (1 μSv/h) and to use enough interior space. The MCNPX2.5 code was used and a dose evaluation was performed for the location of the shielding material, shielding structure, and hole structure. The evaluation result differs according to the shielding material location. The dose rate was small when the shielding material was positioned at the center. The dose evaluation result regarding the location of the shielding material was applied to the facility and the shielding thickness was determined (In 50 cm + Borax 5 cm + Out 45cm). In the existing hole structure, the radiation leak is higher than the standard. A hole structure model to prevent leakage of radiation was proposed. The general public dose limit was satisfied when using the concrete reinforcement and a zigzag structure. The shielding result will be of help to the facility shielding optimization.

  7. Field distribution analysis in deflecting structures

    Paramonov, V.V. [Joint Inst. for Nuclear Research, Moscow (Russian Federation)


    Deflecting structures are used now manly for bunch rotation in emittance exchange concepts, bunch diagnostics and to increase the luminosity. The bunch rotation is a transformation of a particles distribution in the six dimensional phase space. Together with the expected transformations, deflecting structures introduce distortions due to particularities - aberrations - in the deflecting field distribution. The distributions of deflecting fields are considered with respect to non linear additions, which provide emittance deteriorations during a transformation. The deflecting field is treated as combination of hybrid waves HE{sub 1} and HM{sub 1}. The criteria for selection and formation of deflecting structures with minimized level of aberrations are formulated and applied to known structures. Results of the study are confirmed by comparison with results of numerical simulations.

  8. Solid Propellant Grain Structural Integrity Analysis


    The structural properties of solid propellant rocket grains were studied to determine the propellant resistance to stresses. Grain geometry, thermal properties, mechanical properties, and failure modes are discussed along with design criteria and recommended practices.


    Lilia Anghel


    Full Text Available The multitude of physiological processes in which the binding of iron ions takes part makes its mechanism worth investigating. The multiple sequence alignment method was applied to investigate the structure similarities of five lactoferrin X-ray crystallographic structures and outline the differences and similarities between lactoferrin and serum transferrin. The results of this study provide useful insights into the mechanism of iron-binding of lactoferrin protein molecule.

  10. Tools for analysis of Dirac structures on banach spaces

    Iftime, Orest V.; Sandovici, Adrian; Golo, Goran


    Power-conserving and Dirac structures are known as an approach to mathematical modeling of physical engineering systems. In this paper connections between Dirac structures and well known tools from standard functional analysis are presented. The analysis can be seen as a possible starting framework

  11. Structural analysis and functional characteristics of greenhouses in ...



    May 24, 2010 ... This study was carried out to determine the structural analysis and functional characteristics of the greenhouses ..... Duncan test results for mean separation of calculated stretch ratios, ... and Organic Fertilizers and Possible Effects in the Greenhouses of ... Structural analysis of greenhouses: A case study in.

  12. Method of morphological analysis of enterprise management organizational structure

    Heorhiadi, N.; Iwaszczuk, N.; Vilhutska, R.


    The essence of the method of morphological analysis of enterprise management organizational structure is described in the article. Setting levels of morphological decomposition and specification of sets of elements are necessary for morphological analysis. Based on empirical research identified factors that influence the formation and use of enterprises management organizational structures.

  13. Using Event Structure Analysis to Understand Planned Social Change

    William Stevenson


    Full Text Available In this article, the authors explore the application of Event Structure Analysis in understanding the linkages between events in planned social change. An illustrative example from the Comprehensive Strategy for Serious, Violent and Chronic Juvenile Offenders is used to highlight the key features of Event Structure Analysis.

  14. Shear Effects in Shakedown Analysis of Offshore Structures

    M.J.Fadaee; H.Saffari; R.Tabatabaei


    In this paper, a formulation for shakedown analysis of elastic-plastic offshore structures under cyclic wave loading is presented. In this formulation, a fast numerical solution method is used, suitable for the Finite Element Method (FEM) analysis of large offshore structures on which shear effects in addition to bending and axial effects are taken into account. The Morison equation is adopted for converting the velocity and acceleration terms into resultant forces and it is extended to consider arbitrary orientations of the structural members. The theoretical methods of the shakedown analysis are discussed in detail and the formulation is applied to an offshore structure to verify the concept employed and its analytical capabilities.

  15. Structural analysis with the finite element method linear statics

    Oñate, Eugenio


    STRUCTURAL ANALYSIS WITH THE FINITE ELEMENT METHOD Linear Statics Volume 1 : The Basis and Solids Eugenio Oñate The two volumes of this book cover most of the theoretical and computational aspects of the linear static analysis of structures with the Finite Element Method (FEM). The content of the book is based on the lecture notes of a basic course on Structural Analysis with the FEM taught by the author at the Technical University of Catalonia (UPC) in Barcelona, Spain for the last 30 years. Volume1 presents the basis of the FEM for structural analysis and a detailed description of the finite element formulation for axially loaded bars, plane elasticity problems, axisymmetric solids and general three dimensional solids. Each chapter describes the background theory for each structural model considered, details of the finite element formulation and guidelines for the application to structural engineering problems. The book includes a chapter on miscellaneous topics such as treatment of inclined supports, elas...

  16. Stochastic Fatigue Analysis of Jacket Type Offshore Structures

    Sigurdsson, Gudfinnur

    In this paper, a stochastic reliability assessment for jacket type offshore structures subjected to wave loads in deep water environments is outlined. In the reliability assessment, structural and loading uncertainties are taken into account by means of some stochastic variables. To estimate...... statistical measures of structural stress variations the modal spectral analysis method is applied....

  17. Probabilistic structural analysis algorithm development for computational efficiency

    Wu, Y.-T.


    The PSAM (Probabilistic Structural Analysis Methods) program is developing a probabilistic structural risk assessment capability for the SSME components. An advanced probabilistic structural analysis software system, NESSUS (Numerical Evaluation of Stochastic Structures Under Stress), is being developed as part of the PSAM effort to accurately simulate stochastic structures operating under severe random loading conditions. One of the challenges in developing the NESSUS system is the development of the probabilistic algorithms that provide both efficiency and accuracy. The main probability algorithms developed and implemented in the NESSUS system are efficient, but approximate in nature. In the last six years, the algorithms have improved very significantly.

  18. Unascertained Factor Method of Dynamic Characteristic Analysis for Antenna Structures

    ZHU Zeng-qing; LIANG Zhen-tao; CHEN Jian-jun


    The dynamic characteristic analysis model of antenna structures is built, in which the structural physical parameters and geometrical dimensions are all considered as unascertained variables, And a structure dynamic characteristic analysis method based on the unascertained factor method is given. The computational expression of structural characteristic is developed by the mathematics expression of unascertained factor and the principles of unascertained rational numbers arithmetic. An example is given, in which the possible values and confidence degrees of the unascertained structure characteristics are obtained. The calculated results show that the method is feasible and effective.

  19. Meta-analysis a structural equation modeling approach

    Cheung, Mike W-L


    Presents a novel approach to conducting meta-analysis using structural equation modeling. Structural equation modeling (SEM) and meta-analysis are two powerful statistical methods in the educational, social, behavioral, and medical sciences. They are often treated as two unrelated topics in the literature. This book presents a unified framework on analyzing meta-analytic data within the SEM framework, and illustrates how to conduct meta-analysis using the metaSEM package in the R statistical environment. Meta-Analysis: A Structural Equation Modeling Approach begins by introducing the impo

  20. Correct use of Membrane Elements in Structural Analysis

    Rothman Timothy


    Full Text Available Structural analysis of consumer electronic devices such as phones and tablets involves Finite Element Analysis (FEA. Dynamic loading conditions such as device dropping and bending dictate accurate FEA models to reduce design risk in many areas. The solid elements typically used in structural analysis do not have integration points on the surface. The outer surface is of most interest because that is where the cracks start. Analysts employ a post processing trick through using membranes to bring accurate stress/strain results to the surface. This paper explains numerical issues with implementation of membranes and recommends a methodology for accurate structural analysis.

  1. Rapid Electromagnetic Analysis of Entire Accelerator Structures

    Cooke, Simon


    We present results of a new method for fast, accurate calculation, in 3-D, of the electromagnetic mode spectrum of long, tapered accelerator structures. Instead of discretizing the entire structure directly and solving a huge matrix eigenvalue problem, we use a new two-step technique that scales much better to long, multi-cavity structures. In the first step we compute a small number of eigenmodes of individual cavities, achieving 0.05% frequency accuracy using a new second-order finite-element code. In the second step we use these 3-D mode solutions as field basis functions to obtain a reduced matrix representation of Maxwell's equations for the complete structure. Solving the reduced system takes just a few minutes on a desktop PC even with more than 100 non-identical cavities, and gives the complete mode spectrum in the first few bands of the structure. By judicious choice of the basis modes, we retain 0.05% frequency accuracy for these global solutions, and can reconstruct the complete 3-D field of each m...

  2. Earthquake analysis of structures using nonlinear models

    Cemalovic, Miran


    Throughout the governing design codes, several different methods are presented for the evaluation of seismic problems. This thesis assesses the non-linear static and dynamic procedures presented in EN 1998-1 through the structural response of a RC wall-frame building. The structure is designed in detail according to the guidelines for high ductility (DCH) in EN 1998-1. The applied procedures are meticulously evaluated and the requirements in EN 1998-1 are reviewed. In addition, the finite ele...

  3. Exact analysis of bi-periodic structures

    Cai, C W; Chan, H C


    By using the U-transformation method, it is possible to uncouple linear simultaneous equations, either algebraic or differential, with cyclic periodicity. This book presents a procedure for applying the U-transformation technique twice to uncouple the two sets of unknown variables in a doubly periodic structure to achieve an analytical exact solution. Explicit exact solutions for the static and dynamic analyses for certain engineering structures with doubly periodic properties - such as a continuous truss with any number of spans, cable network and grillwork on supports with periodicity, and g


    赵晓兵; 方秦


    The structural deformation velocity plays a significant role in the dynamic calculation of underground blast-resistant structures. The motion differentiating equation of a structure system taking into account the role of deformation velocity of the structure will truthfully describe the actual situation of structural vibration. With the one-dimensional plane wave theory, the expression of load on the structural periphery is developed, and the generalized variation principle for the dynamic analysis of underground arched-bar structures is given. At the same time, the results of the numerical calculation are compared.

  5. Structural Analysis Extended with Active Fault Isolation - Methods and Algorithms

    Gelso, Esteban R.; Blanke, Mogens


    on system inputs can considerably enhance fault isolability. This paper investigates this possibility of active fault isolation from a structural point of view. While such extension of the structural analysis approach was suggested earlier, algorithms and case studies were needed to explore this theory....... The paper develops algorithms for investigation of the possibilities of active structural isolation and it offers illustrative examples and a larger case study to explore the properties of active structural isolability ideas....

  6. Earthquake Analysis of Structure by Base Isolation Technique in SAP

    T. Subramani; J. Jothi


    This paper presents an overview of the present state of base isolation techniques with special emphasis and a brief on other techniques developed world over for mitigating earthquake forces on the structures. The dynamic analysis procedure for isolated structures is briefly explained. The provisions of FEMA 450 for base isolated structures are highlighted. The effects of base isolation on structures located on soft soils and near active faults are given in brief. Simple case s...

  7. Total-System Approach To Design And Analysis Of Structures

    Verderaime, V.


    Paper presents overview and study of, and comprehensive approach to, multidisciplinary engineering design and analysis of structures. Emphasizes issues related to design of semistatic structures in environments in which spacecraft launched, underlying concepts applicable to other structures within unique terrestrial, marine, or flight environments. Purpose of study to understand interactions among traditionally separate engineering design disciplines with view toward optimizing not only structure but also overall design process.

  8. RNA structural analysis by evolving SHAPE chemistry.

    Spitale, Robert C; Flynn, Ryan A; Torre, Eduardo A; Kool, Eric T; Chang, Howard Y


    RNA is central to the flow of biological information. From transcription to splicing, RNA localization, translation, and decay, RNA is intimately involved in regulating every step of the gene expression program, and is thus essential for health and understanding disease. RNA has the unique ability to base-pair with itself and other nucleic acids to form complex structures. Hence the information content in RNA is not simply its linear sequence of bases, but is also encoded in complex folding of RNA molecules. A general chemical functionality that all RNAs have is a 2'-hydroxyl group in the ribose ring, and the reactivity of the 2'-hydroxyl in RNA is gated by local nucleotide flexibility. In other words, the 2'-hydroxyl is reactive at single-stranded and conformationally flexible positions but is unreactive at nucleotides constrained by base-pairing. Recent efforts have been focused on developing reagents that modify RNA as a function of RNA 2' hydroxyl group reactivity. Such RNA structure probing techniques can be read out by primer extension in experiments termed RNA SHAPE (selective 2'- hydroxyl acylation and primer extension). Herein, we describe the efforts devoted to the design and utilization of SHAPE probes for characterizing RNA structure. We also describe current technological advances that are being applied to utilize SHAPE chemistry with deep sequencing to probe many RNAs in parallel. The merging of chemistry with genomics is sure to open the door to genome-wide exploration of RNA structure and function.

  9. Failure Analysis of Composite Structure Materials.


    8MATERIAL STRUCTURES DISCONTINUITY T•R PLY DROPOFF i 7ARC LAP/GAP . PRPAATION A, ,OM LY , 1e, ’ •INS ERVICE MAINTENANCE DAMAGE SVv , S IMPACT \\\\ CHESIE ...composite joints such as box beam members, for example, are difficult to inspect by ultrasonic techniques, and the X-ray attenuation coefficients of

  10. Spatial Analysis Of Human Capital Structures

    Gajdos Artur


    Full Text Available The main purpose of this paper is to analyse the interdependence between labour productivity and the occupational structure of human capital in a spatial cross-section. Research indicates (see Fischer 2009 the possibility to assess the impact of the quality of human capital (measured by means of the level of education on labour productivity in a spatial cross-section.

  11. RNA Structural Analysis by Evolving SHAPE Chemistry

    Spitale, Robert C.; Flynn, Ryan A.; Torre, Eduardo A.; Kool, Eric T.; Chang, Howard Y.


    RNA is central to the flow of biological information. From transcription to splicing, RNA localization, translation, and decay, RNA is intimately involved in regulating every step of the gene expression program, and is thus essential for health and understanding disease. RNA has the unique ability to base-pair with itself and other nucleic acids to form complex structures. Hence the information content in RNA is not simply its linear sequence of bases, but is also encoded in complex folding of RNA molecules. A general chemical functionality that all RNAs have is a 2’-hydroxyl group in the ribose ring, and the reactivity of the 2'-hydroxyl in RNA is gated by local nucleotide flexibility. In other words, the 2'-hydroxyl is reactive at single-stranded and conformationally flexible positions but is unreactive at nucleotides constrained by base pairing. Recent efforts have been focused on developing reagents that modify RNA as a function of RNA 2’ hydroxyl group flexibility. Such RNA structure probing techniques can be read out by primer extension in experiments termed RNA SHAPE (Selective 2’ Hydroxyl Acylation and Primer Extension). Herein we describe the efforts devoted to the design and utilization of SHAPE probes for characterizing RNA structure. We also describe current technological advances that are being used to utilize SHAPE chemistry with deep sequencing to probe many RNAs in parallel. The merger of chemistry with genomics is sure to open the door to genome-wide exploration of RNA structure and function. PMID:25132067

  12. Stochastic finite element analysis of steel structures

    Waarts, P.H.; Vrouwenvelder, A.C.W.M.


    The paper describes two well known but adapted methods for calculating the probability of failure of structures. First the First Order Reliability Method (FORM) is described using an adapted response surface. Secondly the Directional Sampling (DS) procedure is described using truncated stochastic di

  13. Structure function analysis of mammalian cryptochromes

    F. Tamanini (Filippo); I. Chaves (Ines); M.I. Bajek (Monika); G.T.J. van der Horst (Gijsbertus)


    textabstractMembers of the photolyase/cryptochrome family are flavoproteins that share an extraordinary conserved core structure (photolyase homology region, PHR), but the presence of a carboxy-terminal extension is limited to the cryptochromes. Photolyases are DNA-repair enzymes that remove

  14. Reliability Analysis of an Offshore Structure

    Sørensen, John Dalsgaard; Thoft-Christensen, Palle; Rackwitz, R.


    A jacket type offshore structure from the North Sea is considered. The time variant reliability is estimated for failure defined as brittie fradure and crack through the tubular roerober walls. The stochastic modeiling is described. The hot spot stress speetral moments as fundion of the stochastic...

  15. Structural Analysis of a Dragonfly Wing

    Jongerius, S.R.; Lentink, D.


    Dragonfly wings are highly corrugated, which increases the stiffness and strength of the wing significantly, and results in a lightweight structure with good aerodynamic performance. How insect wings carry aerodynamic and inertial loads, and how the resonant frequency of the flapping wings is tuned

  16. Design and analysis of composite structures with applications to aerospace structures

    Kassapoglou, Christos


    Design and Analysis of Composite Structures enables graduate students and engineers to generate meaningful and robust designs of complex composite structures. Combining analysis and design methods for structural components, the book begins with simple topics such as skins and stiffeners and progresses through to entire components of fuselages and wings. Starting with basic mathematical derivation followed by simplifications used in real-world design, Design and Analysis of Composite Structures presents the level of accuracy and range of applicability of each method. Examples taken from ac

  17. Structural Analysis Using NX Nastran 9.0

    Rolewicz, Benjamin M.


    NX Nastran is a powerful Finite Element Analysis (FEA) software package used to solve linear and non-linear models for structural and thermal systems. The software, which consists of both a solver and user interface, breaks down analysis into four files, each of which are important to the end results of the analysis. The software offers capabilities for a variety of types of analysis, and also contains a respectable modeling program. Over the course of ten weeks, I was trained to effectively implement NX Nastran into structural analysis and refinement for parts of two missions at NASA's Kennedy Space Center, the Restore mission and the Orion mission.

  18. Seeking Development in the Succession-An Analysis of Saussure's Structuralism and Strauss' Cultural Structuralism



    With an analysis of Saussure's linguistic structuralism and Strauss' cultural structuralism and exploration of their great impact on linguistic and cultural theory respectively, the author of this paper can safely draw the conclusion that "Saussure's perspective was to provide an intellectual seed that was to flower in later decades as structuralism became a general approach to the study of culture". Saussure, together

  19. Isogeometric analysis for thin-walled composite structures

    Guo, Y.


    The conceptual ideas behind isogeometric analysis (IGA) are aimed at unifying computer aided design (CAD) and finite element analysis (FEA). Isogeometric analysis employs the non-uniform rational B-spline functions (NURBS) used for the geometric description of a structure to approximate its physical

  20. Impact factors of fractal analysis of porous structure


    Characterization of pore structure is one of the key problems for fabrication and application research on porous materials. But, complexity of pore structure makes it difficult to characterize pore structure by Euclidean geometry and traditional experimental methods. Fractal theory has been proved effective to characterize the complex pore structure. The box dimension method based on fractal theory was applied to characterizing the pore structure of fiber porous materials by analyzing the electronic scanning microscope (SEM) images of the porous materials in this paper. The influences of image resolution, threshold value, and image magnification on fractal analysis were investigated. The results indicate that such factors greatly affect fractal analysis process and results. The appropriate magnification threshold and fractal analysis are necessary for fractal analysis.

  1. Large-scale structural analysis: The structural analyst, the CSM Testbed and the NAS System

    Knight, Norman F., Jr.; Mccleary, Susan L.; Macy, Steven C.; Aminpour, Mohammad A.


    The Computational Structural Mechanics (CSM) activity is developing advanced structural analysis and computational methods that exploit high-performance computers. Methods are developed in the framework of the CSM testbed software system and applied to representative complex structural analysis problems from the aerospace industry. An overview of the CSM testbed methods development environment is presented and some numerical methods developed on a CRAY-2 are described. Selected application studies performed on the NAS CRAY-2 are also summarized.

  2. In Depth Analysis of Food Structures

    Nielsen, Otto Højager Attermann; Dahl, Anders Lindbjerg; Larsen, Rasmus


    In this paper we describe a computer vision system based on SLS (Subsurface Laser Scattering) for industrial food inspection. To ob- tain high and uniform quality, in for example dairy products like yoghurt and cheese, it is important to monitor the change in size and shape of microscopic particles...... over time. In this paper we demonstrate the use- fulness of our SLS system for characterizing food items. We use a laser source that can be tuned to any wavelength in the range of 455 nm - 1020 nm by applying an AOTF (Acousto-Optical Tunable Filter) to an optical beam generated by a Super......K (supercontinuum) laser system. In our experiments we show how the system can be used for discriminating dairy products with dierent structure and how the structural change of a foam can be monitored over time. Time stability of the system is essential for measurements over several hours, and we demonstrate...

  3. Truncated Moment Analysis of Nucleon Structure Functions

    A. Psaker; W. Melnitchouk; M. E. Christy; C. E. Keppel


    We employ a novel new approach using "truncated" moments, or integrals of structure functions over restricted regions of x, to study local quark-hadron duality, and the degree to which individual resonance regions are dominated by leading twists. Because truncated moments obey the same Q^2 evolution equations as the leading twist parton distributions, this approach makes possible for the first time a description of resonance region data and the phenomenon of quark-hadron duality directly from QCD.

  4. Analysis and Synthesis of Robust Data Structures


    1.3.2 Multiversion Software. .. .. .. .. .. .... .. ... .. ...... 5 1.3.3 Robust Data Structure .. .. .. .. .. .. .. .. .. ... .. ..... 6 1.4...context are 0 multiversion software, which is an adaptation oi N-modulo redundancy (NMR) tech- nique. * recovery blocks, which is an adaptation of...implementations using these features for such a hybrid approach. 1.3.2 Multiversion Software Avizienis [AC77] was the first to adapt NMR technique into

  5. Finite Element Analysis of Eutectic Structures


    by solid state sintering . The microstructure can exhibit many geometrical forms: dendritic, lamellar, and rods. The self-assembled structure can be...Thermal data was measured in the temperature range of 373 to 1273 K by the laser flash method (Anter Flashline 5000, Anter Corporation, Pittsburgh, PA...therefore is not believed to alter thermal measurements significantly. Specimens were also coated with carbon 4 paint in accordance with standard flash

  6. Structural Analysis of a Dragonfly Wing

    Jongerius, S.R.; Lentink, D.


    Dragonfly wings are highly corrugated, which increases the stiffness and strength of the wing significantly, and results in a lightweight structure with good aerodynamic performance. How insect wings carry aerodynamic and inertial loads, and how the resonant frequency of the flapping wings is tuned for carrying these loads, is however not fully understood. To study this we made a three-dimensional scan of a dragonfly (Sympetrum vulgatum) fore- and hindwing with a micro-CT scanner. The scans c...

  7. Numerical Limit Analysis of Precast Concrete Structures

    Herfelt, Morten Andersen; Poulsen, Peter Noe; Hoang, Linh Cao


    optimisation as well as material optimisation is given and a four-storey shear wall is analysed using load optimisation. The analysis yields a capacity more than three times larger than the design load for the critical load case, and the collapse mode and stress distribution are analysed. Finally, numerical...

  8. Modal analysis of kagome-lattice structures

    Perez, H.; Blakley, S.; Zheltikov, A. M.


    The first few lowest order circularly symmetric electromagnetic eigenmodes of a full kagome lattice are compared to those of a kagome lattice with a hexagonal defect. This analysis offers important insights into the physics behind the waveguiding properties of hollow-core fibers with a kagome-lattice cladding.

  9. Protein structure similarity from principle component correlation analysis

    Chou James


    Full Text Available Abstract Background Owing to rapid expansion of protein structure databases in recent years, methods of structure comparison are becoming increasingly effective and important in revealing novel information on functional properties of proteins and their roles in the grand scheme of evolutionary biology. Currently, the structural similarity between two proteins is measured by the root-mean-square-deviation (RMSD in their best-superimposed atomic coordinates. RMSD is the golden rule of measuring structural similarity when the structures are nearly identical; it, however, fails to detect the higher order topological similarities in proteins evolved into different shapes. We propose new algorithms for extracting geometrical invariants of proteins that can be effectively used to identify homologous protein structures or topologies in order to quantify both close and remote structural similarities. Results We measure structural similarity between proteins by correlating the principle components of their secondary structure interaction matrix. In our approach, the Principle Component Correlation (PCC analysis, a symmetric interaction matrix for a protein structure is constructed with relationship parameters between secondary elements that can take the form of distance, orientation, or other relevant structural invariants. When using a distance-based construction in the presence or absence of encoded N to C terminal sense, there are strong correlations between the principle components of interaction matrices of structurally or topologically similar proteins. Conclusion The PCC method is extensively tested for protein structures that belong to the same topological class but are significantly different by RMSD measure. The PCC analysis can also differentiate proteins having similar shapes but different topological arrangements. Additionally, we demonstrate that when using two independently defined interaction matrices, comparison of their maximum

  10. Global Analysis and Structural Performance of the Tubed Mega Frame

    Zhang, Han


    The Tubed Mega Frame is a new structure concept for high-rise buildings which is developed by Tyréns. In order to study the structural performance as well as the efficiency of this new concept, a global analysis of the Tubed Mega Frame structure is performed using finite element analysis software ETABS. Besides, the lateral loads that should be applied on the structure according to different codes are also studied. From the design code study for wind loads and seismic design response spectrum...

  11. Nonlinear dynamic analysis of quasi-symmetric anisotropic structures

    Noor, Ahmed K.; Peters, Jeanne M.


    An efficient computational method for the nonlinear dynamic analysis of quasi-symmetric anisotropic structures is proposed. The application of mixed models simplifies the analytical development and improves the accuracy of the response predictions, and operator splitting allows the reduction of the analysis model of the quasi-symmetric structure to that of the corresponding symmetric structure. The preconditoned conjugate gradient provides a stable and effective technique for generating the unsymmetric response of the structure as the sum of a symmetrized response plus correction modes. The effectiveness of the strategy is demonstrated with the example of a laminated anisotropic shallow shell of quadrilateral planform subjected to uniform normal loading.

  12. Acoustic design sensitivity analysis of structural sound radiation



    This paper presents an acoustic design sensitivity(ADS)analysis on sound radiation of structures by using the boundary element method(BEM).We calculated the velocity distribution of the thin plate by analytical method and the surface sound pressure by Rayleigh integral,and expressed the sound radiation power of the structure in a positive definite quadratic form of the Hermitian with an impedance matrix.The ADS analysis of the plate was thus translated into the analysis of structure dynamic sensitivity and ...

  13. The dream of Irma's injection: a structural analysis.

    Kuper, A; Stone, A A


    Lévi-Strauss developed a radically new, structural approach to the analysis of myth that may be adapted for use in the analysis of dreams. The method provides an alternative to psychoanalytic dream interpretation, focusing particularly on the internal dialectic of the dream--the movement from the initial premise to the resolution. This emphasis on the internal coherence and structured development of the dream contrasts strongly with the Freudian piecemeal decoding of dreams. The method is exemplified by analysis of Freud's classic Irma dream; it reveals a structure and message that have hitherto remained obscure. The interpretation connects the dream to Freud's theoretical ideas rather than to his supposed psychosexual fixations.

  14. ANOVA like analysis for structured families of stochastic matrices

    Dias, Cristina; Santos, Carla; Varadinov, Maria; Mexia, João T.


    Symmetric stochastic matrices width a width a dominant eigenvalue λ and the corresponding eigenvector α appears in many applications. Such matrices can be written as M =λ α αt+E¯. Thus β = λ α will be the structure vector. When the matrices in such families correspond to the treatments of a base design we can carry out a ANOVA like analysis of the action of the treatments in the model on the structured vectors. This analysis can be transversal-when we worked width homologous components and - longitudinal when we consider contrast on the components of each structure vector. The analysis will be briefly considered at the end of our presentation.

  15. Protective Structure Backpacking and In-Structure Shock Isolation Systems Analysis and Design.

    A parametric analysis is used to develop feasible backpacking -structure configurations and to determine the effect of backpacking on the responses of...the study determined the effectiveness of backpacking in attenuating the structure motions and in reducing the peak stresses and peak the structure. The second phase investigated the response of various shock isolation systems to structure input motions from preferred backpacking

  16. Molecular and structural analysis of viscoelastic properties

    Yapp, Rebecca D.; Kalyanam, Sureshkumar; Insana, Michael F.


    Elasticity imaging is emerging as an important tool for breast cancer detection and monitoring of treatment. Viscoelastic image contrast in breast lesions is generated by disease specific processes that modify the molecular structure of connective tissues. We showed previously that gelatin hydrogels exhibit mechanical behavior similar to native collagen found in breast tissue and therefore are suitable as phantoms for elasticity imaging. This paper summarizes our study of the viscoelastic properties of hydrogels designed to discover molecular-scale sources of elasticity image contrast.

  17. Structural analysis of ITER multi-purpose deployer

    Manuelraj, Manoah Stephen, E-mail: [Institute for Plasma Research, Gandhinagar, Gujarat 382428 (India); Dutta, Pramit; Gotewal, Krishan Kumar; Rastogi, Naveen [Institute for Plasma Research, Gandhinagar, Gujarat 382428 (India); Tesini, Alessandro [ITER Organization, Route de Vinon-sur-Verdon, CS 90046, 1306 St Paul lez Durance Cedex (France); Choi, Chang-Hwan, E-mail: [ITER Organization, Route de Vinon-sur-Verdon, CS 90046, 1306 St Paul lez Durance Cedex (France)


    Highlights: • System modelling for structural analysis of the Multi-Purpose Deployer (MPD). • Finite element modeling of the Multi-Purpose Deployer (MPD). • Static, modal and seismic response analysis of the Multi-Purpose Deployer (MPD). • Iterative structural analysis and design update to satisfy the structural criteria. • Modal analysis for various kinematic configurations. • Reaction force calculations on the interfacing systems. - Abstract: The Multi-Purpose Deployer (MPD) is a general purpose ITER in-vessel remote handling (RH) system. The main handling equipment, known as the MPD Transporter, consists of a series of linked bodies, which provide anchoring to the vacuum vessel port and an articulated multi-degree of freedom motion to perform various in-vessel maintenance tasks. During the in-vessel operations, the structural integrity of the system should be guaranteed against various operational and seismic loads. This paper presents the structural analysis results of the concept design of the MPD Transporter considering the seismic events. Static structural, modal and frequency response spectrum analyses have been performed to verify the structural integrity of the system, and to provide reaction forces to the interfacing systems such as vacuum vessel and cask. Iterative analyses and design updates are carried out based on the reference design of the system to improve the structural behavior of the system. The frequency responses of the system in various kinematics and payloads are assessed.

  18. Structural analysis of reactor fuel elements

    Weeks, R.W.


    An overview of fuel-element modeling is presented that traces the development of codes for the prediction of light-water-reactor and fast-breeder-reactor fuel-element performance. It is concluded that although the mathematical analysis is now far advanced, the development and incorporation of mechanistic constitutive equations has not kept pace. The resultant reliance on empirical correlations severely limits the physical insight that can be gained from code extrapolations. Current efforts include modeling of alternate fuel systems, analysis of local fuel-cladding interactions, and development of a predictive capability for off-normal behavior. Future work should help remedy the current constitutive deficiencies and should include the development of deterministic failure criteria for use in design.

  19. Musical Structural Analysis Database Based on GTTM

    Hamanaka, Masatoshi; Hirata, Keiji; Tojo, Satoshi


    This paper, we present the publication of our analysis data and analyzing tool based on the generative theory of tonal music (GTTM). Musical databases such as score databases, instrument sound databases, and musical pieces with standard MIDI files and annotated data are key to advancements in the field of music information technology. We started implementing the GTTM on a computer in 2004 and ever since have collected and publicized test data by musicologists in a step-by-step manner. In our ...

  20. Structure analysis on synthetic emerald crystals

    Lee, Pei-Lun; Lee, Jiann-Shing; Huang, Eugene; Liao, Ju-Hsiou


    Single crystals of emerald synthesized by means of the flux method were adopted for crystallographic analyses. Emerald crystals with a wide range of Cr3+-doping content up to 3.16 wt% Cr2O3 were examined by X-ray single crystal diffraction refinement method. The crystal structures of the emerald crystals were refined to R 1 (all data) of 0.019-0.024 and w R 2 (all data) of 0.061-0.073. When Cr3+ substitutes for Al3+, the main adjustment takes place in the Al-octahedron and Be-tetrahedron. The effect of substitution of Cr3+ for Al3+ in the beryl structure results in progressively lengthening of the Al-O distance, while the length of the other bonds remains nearly unchanged. The substitution of Cr3+ for Al3+ may have caused the expansion of a axis, while keeping the c axis unchanged in the emerald lattice. As a consequence, the Al-O-Si and Al-O-Be bonding angles are found to decrease, while the angle of Si-O-Be increases as the Al-O distance increases during the Cr replacement.

  1. Structural and quantitative analysis of Equisetum alkaloids.

    Cramer, Luise; Ernst, Ludger; Lubienski, Marcus; Papke, Uli; Schiebel, Hans-Martin; Jerz, Gerold; Beuerle, Till


    Equisetum palustre L. is known for its toxicity for livestock. Several studies in the past addressed the isolation and identification of the responsible alkaloids. So far, palustrine (1) and N(5)-formylpalustrine (2) are known alkaloids of E. palustre. A HPLC-ESI-MS/MS method in combination with simple sample work-up was developed to identify and quantitate Equisetum alkaloids. Besides the two known alkaloids six related alkaloids were detected in different Equisetum samples. The structure of the alkaloid palustridiene (3) was derived by comprehensive 1D and 2D NMR experiments. N(5)-Acetylpalustrine (4) was also thoroughly characterized by NMR for the first time. The structure of N(5)-formylpalustridiene (5) is proposed based on mass spectrometry results. Twenty-two E. palustre samples were screened by a HPLC-ESI-MS/MS method after development of a simple sample work-up and in most cases the set of all eight alkaloids were detected in all parts of the plant. A high variability of the alkaloid content and distribution was found depending on plant organ, plant origin and season ranging from 88 to 597mg/kg dried weight. However, palustrine (1) and the alkaloid palustridiene (3) always represented the main alkaloids. For the first time, a comprehensive identification, quantitation and distribution of Equisetum alkaloids was achieved.

  2. Nonlinear transient analysis of joint dominated structures

    Chapman, J. M.; Shaw, F. H.; Russell, W. C.


    A residual force technique is presented that can perform the transient analyses of large, flexible, and joint dominated structures. The technique permits substantial size reduction in the number of degrees of freedom describing the nonlinear structural models and can account for such nonlinear joint phenomena as free-play and hysteresis. In general, joints can have arbitrary force-state map representations but these are used in the form of residual force maps. One essential feature of the technique is to replace the arbitrary force-state maps describing the nonlinear joints with residual force maps describing the truss links. The main advantage of this replacement is that the incrementally small relative displacements and velocities across a joint are not monitored directly thereby avoiding numerical difficulties. Instead, very small and 'soft' residual forces are defined giving a numerically attractive form for the equations of motion and thereby permitting numerically stable integration algorithms. The technique was successfully applied to the transient analyses of a large 58 bay, 60 meter truss having nonlinear joints. A method to perform link testing is also presented.

  3. Data analysis of asymmetric structures advanced approaches in computational statistics

    Saito, Takayuki


    Data Analysis of Asymmetric Structures provides a comprehensive presentation of a variety of models and theories for the analysis of asymmetry and its applications and provides a wealth of new approaches in every section. It meets both the practical and theoretical needs of research professionals across a wide range of disciplines and  considers data analysis in fields such as psychology, sociology, social science, ecology, and marketing. In seven comprehensive chapters this guide details theories, methods, and models for the analysis of asymmetric structures in a variety of disciplines and presents future opportunities and challenges affecting research developments and business applications.

  4. Statistical energy analysis of complex structures, phase 2

    Trudell, R. W.; Yano, L. I.


    A method for estimating the structural vibration properties of complex systems in high frequency environments was investigated. The structure analyzed was the Materials Experiment Assembly, (MEA), which is a portion of the OST-2A payload for the space transportation system. Statistical energy analysis (SEA) techniques were used to model the structure and predict the structural element response to acoustic excitation. A comparison of the intial response predictions and measured acoustic test data is presented. The conclusions indicate that: the SEA predicted the response of primary structure to acoustic excitation over a wide range of frequencies; and the contribution of mechanically induced random vibration to the total MEA is not significant.

  5. Applications of Mass Spectrometry to Structural Analysis of Marine Oligosaccharides

    Yinzhi Lang


    Full Text Available Marine oligosaccharides have attracted increasing attention recently in developing potential drugs and biomaterials for their particular physical and chemical properties. However, the composition and sequence analysis of marine oligosaccharides are very challenging for their structural complexity and heterogeneity. Mass spectrometry (MS has become an important technique for carbohydrate analysis by providing more detailed structural information, including molecular mass, sugar constituent, sequence, inter-residue linkage position and substitution pattern. This paper provides an overview of the structural analysis based on MS approaches in marine oligosaccharides, which are derived from some biologically important marine polysaccharides, including agaran, carrageenan, alginate, sulfated fucan, chitosan, glycosaminoglycan (GAG and GAG-like polysaccharides. Applications of electrospray ionization mass spectrometry (ESI-MS are mainly presented and the general applications of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS are also outlined. Some technical challenges in the structural analysis of marine oligosaccharides by MS have also been pointed out.

  6. Applications of mass spectrometry to structural analysis of marine oligosaccharides.

    Lang, Yinzhi; Zhao, Xia; Liu, Lili; Yu, Guangli


    Marine oligosaccharides have attracted increasing attention recently in developing potential drugs and biomaterials for their particular physical and chemical properties. However, the composition and sequence analysis of marine oligosaccharides are very challenging for their structural complexity and heterogeneity. Mass spectrometry (MS) has become an important technique for carbohydrate analysis by providing more detailed structural information, including molecular mass, sugar constituent, sequence, inter-residue linkage position and substitution pattern. This paper provides an overview of the structural analysis based on MS approaches in marine oligosaccharides, which are derived from some biologically important marine polysaccharides, including agaran, carrageenan, alginate, sulfated fucan, chitosan, glycosaminoglycan (GAG) and GAG-like polysaccharides. Applications of electrospray ionization mass spectrometry (ESI-MS) are mainly presented and the general applications of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are also outlined. Some technical challenges in the structural analysis of marine oligosaccharides by MS have also been pointed out.

  7. Economic analysis of the structure, Integration and Performance of ...

    Economic analysis of the structure, Integration and Performance of Rice Markets in Dekina Local Government Area of Kogi ... Open Access DOWNLOAD FULL TEXT ... The data for the study were collected with the aid of a questionnaire.

  8. Convected transient analysis for large space structures maneuver and deployment

    Housner, J.


    Convected-transient analysis techniques in the finite-element method are used to investigate the deployment and maneuver of large spacecraft structures with multiple-member flexible trusses and frames. Numerical results are presented for several sample problems.

  9. Wheat yield dynamics: a structural econometric analysis.

    Sahin, Afsin; Akdi, Yilmaz; Arslan, Fahrettin


    In this study we initially have tried to explore the wheat situation in Turkey, which has a small-open economy and in the member countries of European Union (EU). We have observed that increasing the wheat yield is fundamental to obtain comparative advantage among countries by depressing domestic prices. Also the changing structure of supporting schemes in Turkey makes it necessary to increase its wheat yield level. For this purpose, we have used available data to determine the dynamics of wheat yield by Ordinary Least Square Regression methods. In order to find out whether there is a linear relationship among these series we have checked each series whether they are integrated at the same order or not. Consequently, we have pointed out that fertilizer usage and precipitation level are substantial inputs for producing high wheat yield. Furthermore, in respect for our model, fertilizer usage affects wheat yield more than precipitation level.

  10. Structural shell analysis understanding and application

    Blaauwendraad, Johan


    The mathematical description of the properties of a shell is much more elaborate than those of beam and plate structures. Therefore many engineers and architects are unacquainted with aspects of shell behaviour and design, and are not familiar with sufficiently reliable shell theories for the different shell types as derived in the middle of the 20th century. Rather than contributing to theory development, this university textbook focuses on architectural and civil engineering schools. Of course, practising professionals will profit from it as well. The book deals with thin elastic shells, in particular with cylindrical, conical and spherical types, and with elliptic and hyperbolic paraboloids. The focus is on roofs, chimneys, pressure vessels and storage tanks. Special attention is paid to edge bending disturbance zones, which is indispensable knowledge in FE meshing. A substantial part of the book results from research efforts in the mid 20th century at Delft University of Technology. As such, it is a valua...

  11. Ellipsometric Analysis of Cadmium Telluride Films’ Structure

    Anna Evmenova; Volodymyr Odarych; Mykola Vuichyk; Fedir Sizov


    Ellipsometric analysis of CdTe films grown on Si and CdHgTe substrates at the “hot-wall” epitaxy vacuum setup has been performed. It has been found that ellipsometric data calculation carried out by using a simple one-layer film model leads to radical distortion of optical constants spectra: this fact authenticates the necessity to attract a more complicated model that should include heterogeneity of films. Ellipsometric data calculation within a two-layer film model permitted to conclude tha...

  12. ‘Designerly’ Analysis of Participation Structures

    Buur, Jacob; Beuthel, Maria Rosa; Caglio, Agnese


    With the inclusion of not only users but stake-holders of many different kinds, design processes turn into complex collaborative challenges. Thus, improving design practices requires research into how people participate and contribute in social interaction. But research methods for understand......-ing such activities tend to be highly analytical and hence difficult for design researchers to engage with, if results are meant to be actionable. Through a series of experiments we develop tangible support for a ‘designerly’ interaction analysis of one important aspect of collaborative design activities...

  13. Push-Over Analysis for Concrete Structures of Tall Building

    朱杰江; 张佩军; 吕西林; 容柏生


    In this paper, push-over analysis for tall concrete structures was made and a corresponding computer program was given.Several kinds of elements in the program were considered to meet the demand of tall buildings with complex structural type. These elements included beam-column element for beams and columns, single slice wall element and three vertical line element for walls, and tube-wall element for tubes. Computational example for verifying the models indicates that the result obtained by this method is identical with a well-known test result and the program can be used to search for the full process of structural reaction, even the softening stage of the structure. With this push-over analysis method, an actual tall building with complex structural type was analyzed, and the result has been put into practice of the structural design of the building.

  14. Discrete Discriminant analysis based on tree-structured graphical models

    Perez de la Cruz, Gonzalo; Eslava, Guillermina

    The purpose of this paper is to illustrate the potential use of discriminant analysis based on tree{structured graphical models for discrete variables. This is done by comparing its empirical performance using estimated error rates for real and simulated data. The results show that discriminant...... analysis based on tree{structured graphical models is a simple nonlinear method competitive with, and sometimes superior to, other well{known linear methods like those assuming mutual independence between variables and linear logistic regression....

  15. Probabilistic Nonlinear Analysis of Reinforced Concrete Bubbler Tower Structure Failure

    Králik Juraj


    Full Text Available This paper describes the reliability analysis of concrete bubbler tower structure of nuclear power plant with the reactor WWER 440 under high internal overpressure. There is showed summary of calculation models and calculation methods for the probability analysis of the structural integrity considering degradation effects and high internal overpressure. The uncertainties of the resistance and the calculation model were taking in the account in the RSM method.

  16. Implementation of efficient sensitivity analysis for optimization of large structures

    Umaretiya, J. R.; Kamil, H.


    The paper presents the theoretical bases and implementation techniques of sensitivity analyses for efficient structural optimization of large structures, based on finite element static and dynamic analysis methods. The sensitivity analyses have been implemented in conjunction with two methods for optimization, namely, the Mathematical Programming and Optimality Criteria methods. The paper discusses the implementation of the sensitivity analysis method into our in-house software package, AutoDesign.

  17. Discrete Discriminant analysis based on tree-structured graphical models

    Perez de la Cruz, Gonzalo; Eslava, Guillermina

    The purpose of this paper is to illustrate the potential use of discriminant analysis based on tree{structured graphical models for discrete variables. This is done by comparing its empirical performance using estimated error rates for real and simulated data. The results show that discriminant a...... analysis based on tree{structured graphical models is a simple nonlinear method competitive with, and sometimes superior to, other well{known linear methods like those assuming mutual independence between variables and linear logistic regression....

  18. Seismic design and analysis of nuclear power plant structures

    Pentti Varpasuo


    The seismic design and analysis of nuclear power plant (NPP) begin with the seismic hazard assessment and design ground motion development for the site.The following steps are needed for the seismic hazard assessment and design ground motion development:a.the development of regional seismo-tectonic model with seismic source areas within 500 km radius centered to the site; b.the development of strong motion prediction equations;c.logic three development for taking into account uncertainties and seismic hazard quantification; d.the development of uniform hazard response spectra for ground motion at the site; e.simulation of acceleration time histories compatible with uniform hazard response spectra.The following phase two in seismic design of NPP structures is the analysis of structural response for the design ground motion.This second phase of the process consists of the following steps:a.development of structural models of the plant buildings; b.development of the soil model underneath the plant buildings for soil-structure interaction response analysis; c.determination of in-structure response spectra for the plant buildings for the equipment response analysis.In the third phase of the seismic design and analysis the equipment is analyzed on the basis of in-structure response spectra.For this purpose the structural models of the mechanical components and piping in the plant are set up.In large 3D-structural models used today the heaviest equipment of the primary coolant circuit is included in the structural model of the reactor building.In the fourth phase the electrical equipment and automation and control equipment are seismically qualified with the aid of the in-structure spectra developed in the phase two using large three-axial shaking tables.For this purpose the smoothed envelope spectra for calculated in-structure spectra are constructed and acceleration time is fitted to these smoothed envelope spectra.

  19. Structural and functional analysis of rice genome

    Akhilesh K. Tyagi; Jitendra P. Khurana; Paramjit Khurana; Saurabh Raghuvanshi; Anupama Gaur; Anita Kapur; Vikrant Gupta; Dibyendu Kumar; V. Ravi; Shubha Vij; Parul Khurana; Sulabha Sharma


    Rice is an excellent system for plant genomics as it represents a modest size genome of 430 Mb. It feeds more than half the population of the world. Draft sequences of the rice genome, derived by whole-genome shotgun approach at relatively low coverage (4–6 X), were published and the International Rice Genome Sequencing Project (IRGSP) declared high quality (>10 X), genetically anchored, phase 2 level sequence in 2002. In addition, phase 3 level finished sequence of chromosomes 1, 4 and 10 (out of 12 chromosomes of rice) has already been reported by scientists from IRGSP consortium. Various estimates of genes in rice place the number at > 50,000. Already, over 28,000 full-length cDNAs have been sequenced, most of which map to genetically anchored genome sequence. Such information is very useful in revealing novel features of macro- and micro-level synteny of rice genome with other cereals. Microarray analysis is unraveling the identity of rice genes expressing in temporal and spatial manner and should help target candidate genes useful for improving traits of agronomic importance. Simultaneously, functional analysis of rice genome has been initiated by marker-based characterization of useful genes and employing functional knock-outs created by mutation or gene tagging. Integration of this enormous information is expected to catalyze tremendous activity on basic and applied aspects of rice genomics.

  20. Structural Analysis of Women’s Heptathlon

    Freya Gassmann


    Full Text Available The heptathlon comprises the results of seven single disciplines, assuming an equal influence from each discipline, depending on the measured performance. Data analysis was based on the data recorded for the individual performances of the 10 winning heptathletes in the World Athletics Championships from 1987 to 2013 and the Olympic Games from 1988 to 2012. In addition to descriptive analysis methods, correlations, bivariate and multivariate linear regressions, and panel data regressions were used. The transformation of the performances from seconds, centimeters, and meters into points showed that the individual disciplines do not equally affect the overall competition result. The currently valid conversion formula for the run, jump, and throw disciplines prefers the sprint and jump disciplines but penalizes the athletes performing in the 800 m run, javelin throw, and shotput disciplines. Furthermore, 21% to 48% of the variance of the sum of points can be attributed to the performances in the disciplines of long jump, 200 m sprint, 100 m hurdles, and high jump. To balance the effects of the single disciplines in the heptathlon, the formula to calculate points should be reevaluated.

  1. Multiscale analysis of structure development in expanded starch snacks

    Sman, van der R.G.M.; Broeze, J.


    In this paper we perform a multiscale analysis of the food structuring process of the expansion of starchy snack foods like keropok, which obtains a solid foam structure. In particular, we want to investigate the validity of the hypothesis of Kokini and coworkers, that expansion is optimal at the mo

  2. Tools for analysis of Dirac structures on Hilbert spaces

    Golo, G.; Iftime, O.V.; Zwart, Heiko J.; van der Schaft, Arjan


    In this paper tools for the analysis of Dirac structures on Hilbert spaces are developed. Some properties are pointed out and two natural representations of Dirac structures on Hilbert spaces are presented. The theory is illustrated on the example of the ideal transmission line.

  3. Reliability-Analysis of Offshore Structures using Directional Loads

    Sørensen, John Dalsgaard; Bloch, Allan; Sterndorff, M. J.


    Reliability analyses of offshore structures such as steel jacket platforms are usually performed using stochastic models for the wave loads based on the omnidirectional wave height. However, reliability analyses with respect to structural failure modes such as total collapse of a structure...... heights from the central part of the North Sea. It is described how the stochastic model for the directional wave heights can be used in a reliability analysis where total collapse of offshore steel jacket platforms is considered....

  4. Ellipsometric Analysis of Cadmium Telluride Films’ Structure

    Anna Evmenova


    Full Text Available Ellipsometric analysis of CdTe films grown on Si and CdHgTe substrates at the “hot-wall” epitaxy vacuum setup has been performed. It has been found that ellipsometric data calculation carried out by using a simple one-layer film model leads to radical distortion of optical constants spectra: this fact authenticates the necessity to attract a more complicated model that should include heterogeneity of films. Ellipsometric data calculation within a two-layer film model permitted to conclude that cadmium telluride films have an outer layer that consists of the three-component mixture of CdTe, cavities, and basic matter oxide. Ratio of mixture components depends on the time of deposition, that is, on the film thickness. The inner layer consists of cadmium telluride.

  5. Sediment Analysis Using a Structured Programming Approach

    Daniela Arias-Madrid


    Full Text Available This paper presents an algorithm designed for the analysis of a sedimentary sample of unconsolidated material and seeks to identify very quickly the main features that occur in a sediment and thus classify them fast and efficiently. For this purpose, it requires that the weight of each particle size to be entered in the program and using the method of Moments, which is based on four equations representing the mean, standard deviation, skewness and kurtosis, is found the attributes of the sample in few seconds. With the program these calculations are performed in an effective and more accurately way, obtaining also the explanations of the results of the features such as grain size, sorting, symmetry and origin, which helps to improve the study of sediments and in general the study of sedimentary rocks.

  6. Structural model analysis of multiple quantitative traits.

    Renhua Li


    Full Text Available We introduce a method for the analysis of multilocus, multitrait genetic data that provides an intuitive and precise characterization of genetic architecture. We show that it is possible to infer the magnitude and direction of causal relationships among multiple correlated phenotypes and illustrate the technique using body composition and bone density data from mouse intercross populations. Using these techniques we are able to distinguish genetic loci that affect adiposity from those that affect overall body size and thus reveal a shortcoming of standardized measures such as body mass index that are widely used in obesity research. The identification of causal networks sheds light on the nature of genetic heterogeneity and pleiotropy in complex genetic systems.

  7. Tanker Structural Analysis for Minor Collisions


    masses (including virtual masses of the water), the initial bearings, and velocities. 2. Throughout the analysis, the bow of the striking ship Is...initial velocity of the striking ship: m( F ______ (1 t 0 V20) =0.0 f= virtual mass, ship I 2 = virtual mass, ship 2~ m I V . V f ’ K’ Jt 11 FIUR m-2...situ .K. 5Y( i~ lZAKD bow $H 14ILIQj P Lh5T I C. EQ k Y kps) 0 0 94-1 W~IAk) J~~E 0 oC4O.1 ,I Hi PL~c,1C. tlA% ;OQ)- AULA / ;0) 4 4 4~IN -c Q

  8. A method for rapid similarity analysis of RNA secondary structures

    Liu Na


    Full Text Available Abstract Background Owing to the rapid expansion of RNA structure databases in recent years, efficient methods for structure comparison are in demand for function prediction and evolutionary analysis. Usually, the similarity of RNA secondary structures is evaluated based on tree models and dynamic programming algorithms. We present here a new method for the similarity analysis of RNA secondary structures. Results Three sets of real data have been used as input for the example applications. Set I includes the structures from 5S rRNAs. Set II includes the secondary structures from RNase P and RNase MRP. Set III includes the structures from 16S rRNAs. Reasonable phylogenetic trees are derived for these three sets of data by using our method. Moreover, our program runs faster as compared to some existing ones. Conclusion The famous Lempel-Ziv algorithm can efficiently extract the information on repeated patterns encoded in RNA secondary structures and makes our method an alternative to analyze the similarity of RNA secondary structures. This method will also be useful to researchers who are interested in evolutionary analysis.

  9. Modelization and structural analysis of FDM parts

    Martínez, J.; Diéguez, J. L.; Ares, J. E.; Pereira, A.; Pérez, J. A.


    Get prototypes from technologies of Rapid Prototyping (RP) is a very important step for the development of new products. In some cases, these prototypes have mechanical properties lower than the final product, which prevents the designers to use all of the potential that these technologies can provide. In this study the RP technology known as FDM (Fused Deposition Modeling) was used to manufacture samples used in tests, in where the orientation of deposition wires in layers were varying depending on manufacturing placement. Mechanical tests were performed to verify the stiffness of the final pieces obtained. The Classical Theory of Laminates (TCL) will be used to predict the mechanical behavior of the parts in different orientations of manufacturing. Thus, this study aims to evaluate the influence of the strategies in the deposition of construction material on the mechanical properties of parts obtained by the FDM and analyzes manufacturing factors for a future generation of a finite elements analytic model that could be used to obtain the structural behavior of parts made by rapid prototyping with FDM technology.

  10. Analysis of boron carbides' electronic structure

    Howard, Iris A.; Beckel, Charles L.


    The electronic properties of boron-rich icosahedral clusters were studied as a means of understanding the electronic structure of the icosahedral borides such as boron carbide. A lower bound was estimated on bipolaron formation energies in B12 and B11C icosahedra, and the associated distortions. While the magnitude of the distortion associated with bipolaron formation is similar in both cases, the calculated formation energies differ greatly, formation being much more favorable on B11C icosahedra. The stable positions of a divalent atom relative to an icosahedral borane was also investigated, with the result that a stable energy minimum was found when the atom is at the center of the borane, internal to the B12 cage. If incorporation of dopant atoms into B12 cages in icosahedral boride solids is feasible, novel materials might result. In addition, the normal modes of a B12H12 cluster, of the C2B10 cage in para-carborane, and of a B12 icosahedron of reduced (D sub 3d) symmetry, such as is found in the icosahedral borides, were calculated. The nature of these vibrational modes will be important in determining, for instance, the character of the electron-lattice coupling in the borides, and in analyzing the lattice contribution to the thermal conductivity.


    Slavko Obadov


    Full Text Available Conditional preparation is a constituent part of overall sports preparation. Conditional training might be defined as a process of improvement of a sportsman’s functional and motor abilities, morphological characteristics, health, as well as the required motor knowledge. Conditional preparation can be might be classified as: general, basic and situational conditional preparation. Programs of the conditional training might be classified as: developing, resuming, recovering, preventive and recovering ones. High level of the general physical preparation enables maximum demonstration of the physical abilities of a sportsman during the stage of improvement of the specific motor abilities. Good general preparation of a sportsman enables him to push beyond his functional limits in order to cope with heavy loads easier, which subsequently enables him to achieve top performance level. Basic conditional preparation assumes the development of the most important judo abilities. Specific conditional preparation is related directly to the execution of different structural elements under the conditional requirements. Situational conditional preparation enables integration of the tactical and conditional training.

  12. Structure analysis of active components of traditional Chinese medicines

    Zhang, Wei; Sun, Qinglei; Liu, Jianhua


    Traditional Chinese Medicines (TCMs) have been widely used for healing of different health problems for thousands of years. They have been used as therapeutic, complementary and alternative medicines. TCMs usually consist of dozens to hundreds of various compounds, which are extracted from raw...... samples. NMR, on the other hand, provides not only information of primary structures but also information of higher order structures, complementing the components structure analysis by HPLC-MS. The most recent progress in the analysis of the commonly used TCMs will be summarized...

  13. Structural mode significance using INCA. [Interactive Controls Analysis computer program

    Bauer, Frank H.; Downing, John P.; Thorpe, Christopher J.


    Structural finite element models are often too large to be used in the design and analysis of control systems. Model reduction techniques must be applied to reduce the structural model to manageable size. In the past, engineers either performed the model order reduction by hand or used distinct computer programs to retrieve the data, to perform the significance analysis and to reduce the order of the model. To expedite this process, the latest version of INCA has been expanded to include an interactive graphical structural mode significance and model order reduction capability.

  14. Structural mode significance using INCA. [Interactive Controls Analysis computer program

    Bauer, Frank H.; Downing, John P.; Thorpe, Christopher J.


    Structural finite element models are often too large to be used in the design and analysis of control systems. Model reduction techniques must be applied to reduce the structural model to manageable size. In the past, engineers either performed the model order reduction by hand or used distinct computer programs to retrieve the data, to perform the significance analysis and to reduce the order of the model. To expedite this process, the latest version of INCA has been expanded to include an interactive graphical structural mode significance and model order reduction capability.

  15. Structural analysis of cell wall polysaccharides using PACE

    Mortimer, Jennifer C. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Institute


    The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. Many techniques to characterize polysaccharide structure are complicated, requiring expensive equipment and specialized operators e.g. NMR, MALDI-MS. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al. 2002). Whilst the method here describes xylan analysis, it can be applied (by use of the appropriate glycosyl hydrolase) to any cell wall polysaccharide.

  16. Structural analysis of Aircraft fuselage splice joint

    Udaya Prakash, R.; Kumar, G. Raj; Vijayanandh, R.; Senthil Kumar, M.; Ramganesh, T.


    In Aviation sector, composite materials and its application to each component are one of the prime factors of consideration due to the high strength to weight ratio, design flexibility and non-corrosive so that the composite materials are widely used in the low weight constructions and also it can be treated as a suitable alternative to metals. The objective of this paper is to estimate and compare the suitability of a composite skin joint in an aircraft fuselage with different joints by simulating the displacement, normal stress, vonmises stress and shear stress with the help of numerical solution methods. The reference Z-stringer component of this paper is modeled by CATIA and numerical simulation is carried out by ANSYS has been used for splice joint presents in the aircraft fuselage with three combinations of joints such as riveted joint, bonded joint and hybrid joint. Nowadays the stringers are using to avoid buckling of fuselage skin, it has joined together by rivets and they are connected end to end by splice joint. Design and static analysis of three-dimensional models of joints such as bonded, riveted and hybrid are carried out and results are compared.

  17. Neighborhood structure effects on the Dynamic response of soil-structure interaction by harmonic analysis

    Pan Dan-guang


    Full Text Available For realizing the variation of structural dynamic characteristics due to neighbor structure in buildings group, the surface structure is idealized as an equivalent single degree of freedom system with rigid base whose site consists of a single homogeneous layer. Based on the model, a equivalent method on the equivalent seismic excitation is proposed. Then, the differences of seismic response and equivalent seismic input between soil - structure interaction (SSI system and structure -soil-structure interaction (SSSI system are investigated by harmonic analysis. The numerical results show that dynamic responses would be underestimated in SSSI system when the forcing frequencies are close to the Natural frequency if the effects of neighborhood structure were ignored. Neighborhood structure would make the translational displacement increase and rocking vibration decrease. When establishing an effective seismic input, it is necessary to consider the impact of inertia interaction.

  18. Advanced analysis and design for fire safety of steel structures

    Li, Guoqiang


    Advanced Analysis and Design for Fire Safety of Steel Structures systematically presents the latest findings on behaviours of steel structural components in a fire, such as the catenary actions of restrained steel beams, the design methods for restrained steel columns, and the membrane actions of concrete floor slabs with steel decks. Using a systematic description of structural fire safety engineering principles, the authors illustrate the important difference between behaviours of an isolated structural element and the restrained component in a complete structure under fire conditions. The book will be an essential resource for structural engineers who wish to improve their understanding of steel buildings exposed to fires. It is also an ideal textbook for introductory courses in fire safety for master’s degree programs in structural engineering, and is excellent reading material for final-year undergraduate students in civil engineering and fire safety engineering. Furthermore, it successfully bridges th...

  19. Structural analysis for diagnosis with application to ship propulsion problem

    Izadi-Zamanabadi, Roozbeh; Blanke, Mogens


    Aiming at design of algorithms for fault diagnosis, structural analysis of systems offers concise yet easy overall analysis. Graph-based matching, which is the essential tech-nique to obtain redundant information for diagnosis, is reconsidered in this paper. Matching is reformulated as a problem ...

  20. Structural analysis for diagnosis with application to ship propulsion problem

    Izadi-Zamanabadi, Roozbeh; Blanke, Mogens


    Aiming at design of algorithms for fault diagnosis, structural analysis of systems offers concise yet easy overall analysis. Graph-based matching, which is the essential tech-nique to obtain redundant information for diagnosis, is reconsidered in this paper. Matching is reformulated as a problem...

  1. Fluid-structure coupled analysis of underwater cylindrical shells

    AI Shang-mao; SUN Li-ping


    Underwater cylindrical shell structures have been found a wide of application in many engineering fields,such as the element of marine,oil platforms,etc.The coupled vibration analysis is a hot issue for these underwater structures.The vibration characteristics of underwater structures are influenced not only by hydrodynamic pressure but also by hydrostatic pressure corresponding to different water depths.In this study,an acoustic finite element method was used to evaluate the underwater structures.Taken the hydrostatic pressure into account in terms of initial stress stiffness,an acoustical fluid-structure coupled analysis of underwater cylindrical shells has been made to study the effect of hydrodynamic pressures on natural frequency and sound radiation.By comparing with the frequencies obtained by the acoustic finite element method and by the added mass method based on the Bessel function,the validity of present analysis was checked.Finally,test samples of the sound radiation of stiffened cylindrical shells were acquired by a harmonic acoustic analysis.The results showed that hydrostatic pressure plays an important role in determining a large submerged body motion,and the characteristics of sound radiation change with water depth. Furthermore,the analysis methods and the results are of significant reference value for studies of other complicated submarine structures.

  2. Structure optimization and simulation analysis of the quartz micromachined gyroscope

    Wu, Xuezhong; Wang, Haoxu; Xie, Liqiang; Dong, Peitao


    Structure optimization and simulation analysis of the quartz micromachined gyroscope are reported in this paper. The relationships between the structure parameters and the frequencies of work mode were analysed by finite element analysis. The structure parameters of the quartz micromachined gyroscope were optimized to reduce the difference between the frequencies of the drive mode and the sense mode. The simulation results were proved by testing the prototype gyroscope, which was fabricated by micro-electromechanical systems (MEMS) technology. Therefore, the frequencies of the drive mode and the sense mode can match each other by the structure optimization and simulation analysis of the quartz micromachined gyroscope, which is helpful in the design of the high sensitivity quartz micromachined gyroscope.

  3. Rapid structural analysis of nanomaterials in aqueous solutions

    Ryuzaki, Sou; Tsutsui, Makusu; He, Yuhui; Yokota, Kazumichi; Arima, Akihide; Morikawa, Takanori; Taniguchi, Masateru; Kawai, Tomoji


    Rapid structural analysis of nanoscale matter in a liquid environment represents innovative technologies that reveal the identities and functions of biologically important molecules. However, there is currently no method with high spatio-temporal resolution that can scan individual particles in solutions to gain structural information. Here we report the development of a nanopore platform realizing quantitative structural analysis for suspended nanomaterials in solutions with a high z-axis and xy-plane spatial resolution of 35.8 ± 1.1 and 12 nm, respectively. We used a low thickness-to-diameter aspect ratio pore architecture for achieving cross sectional areas of analyte (i.e. tomograms). Combining this with multiphysics simulation methods to translate ionic current data into tomograms, we demonstrated rapid structural analysis of single polystyrene (Pst) beads and single dumbbell-like Pst beads in aqueous solutions.

  4. Structure optimization and simulation analysis of the quartz micromachined gyroscope

    Xuezhong Wu


    Full Text Available Structure optimization and simulation analysis of the quartz micromachined gyroscope are reported in this paper. The relationships between the structure parameters and the frequencies of work mode were analysed by finite element analysis. The structure parameters of the quartz micromachined gyroscope were optimized to reduce the difference between the frequencies of the drive mode and the sense mode. The simulation results were proved by testing the prototype gyroscope, which was fabricated by micro-electromechanical systems (MEMS technology. Therefore, the frequencies of the drive mode and the sense mode can match each other by the structure optimization and simulation analysis of the quartz micromachined gyroscope, which is helpful in the design of the high sensitivity quartz micromachined gyroscope.

  5. Automated analysis of fundamental features of brain structures.

    Lancaster, Jack L; McKay, D Reese; Cykowski, Matthew D; Martinez, Michael J; Tan, Xi; Valaparla, Sunil; Zhang, Yi; Fox, Peter T


    Automated image analysis of the brain should include measures of fundamental structural features such as size and shape. We used principal axes (P-A) measurements to measure overall size and shape of brain structures segmented from MR brain images. The rationale was that quantitative volumetric studies of brain structures would benefit from shape standardization as had been shown for whole brain studies. P-A analysis software was extended to include controls for variability in position and orientation to support individual structure spatial normalization (ISSN). The rationale was that ISSN would provide a bias-free means to remove elementary sources of a structure's spatial variability in preparation for more detailed analyses. We studied nine brain structures (whole brain, cerebral hemispheres, cerebellum, brainstem, caudate, putamen, hippocampus, inferior frontal gyrus, and precuneus) from the 40-brain LPBA40 atlas. This paper provides the first report of anatomical positions and principal axes orientations within a standard reference frame, in addition to "shape/size related" principal axes measures, for the nine brain structures from the LPBA40 atlas. Analysis showed that overall size (mean volume) for internal brain structures was preserved using shape standardization while variance was reduced by more than 50%. Shape standardization provides increased statistical power for between-group volumetric studies of brain structures compared to volumetric studies that control only for whole brain size. To test ISSN's ability to control for spatial variability of brain structures we evaluated the overlap of 40 regions of interest (ROIs) in a standard reference frame for the nine different brain structures before and after processing. Standardizations of orientation or shape were ineffective when not combined with position standardization. The greatest reduction in spatial variability was seen for combined standardizations of position, orientation and shape. These

  6. Structural analysis of superconducting dipole prototype for HIAF

    Zhang, Xiaoying; Ni, Dongsheng; Chen, Yuquan; Wu, Wei; Ma, Lizhen


    The High Intensity Heavy-Ion Accelerator Facility is a new project in the Institute of Modern Physics. The dipole magnets of all rings are conceived as fast cycled superconducting magnet with high magnetic field and large gap, the warm iron and superconducting coil structure (superferric) is adopted. The reasonable structure design of coil and cryostat is very important for reliable operation. Based on the finite element software ANSYS, the mechanical analysis of electromagnetic stress, the thermal stress in the cooling down and the stress in the pumping are showed in detail. According to the analysis result, the supporter structure is the key problem of coil system. With reasonable support's structure design, the stress and the deformation of coil structure can be reduced effectively, which ensure the stable operation of superconducting coil system.

  7. Stress Analysis of Ellipsoidal Shell with Inner Guide Structure

    Wei-Wei Zhang; Xiao-Song Wang; Meng Chen; Shi-Jian Yuan


    In order to overcome stress concentration and increase fatigue life of ellipsoidal shells with inner guide structure, the stress analysis for strength check is very important. Owing to the main sectional profile with ellipsoidal shape, the stress distribution for perfect ellipsoidal shell is firstly conducted based on the theoretical calculation and strain gauges measurement. The experiment results show that the stresses increase gradually from pole region to equatorial plane, but still within elastic range. Secondly, strain gauge measurement for ellipsoidal shells with inner guide structure is conducted. The results show that stresses are concentrated at the vicinity of bottom plate and beyond elastic range, so the structural redesign is needed. Finally based on the analysis mentioned above, a redesigned structure with local thickening is proposed. Experimental research shows that the stress varies more even after structural redesign and within allowable range. Numerical simulation shows that both the deformation and fatigue life after redesign are acceptable.

  8. Mutagenicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) in the gill and hepatopancreas of rpsL transgenic zebrafish.

    Amanuma, Kimiko; Tone, Suguru; Nagaya, Masato; Matsumoto, Michi; Watanabe, Tetsushi; Totsuka, Yukari; Wakabayashi, Keiji; Aoki, Yasunobu


    We examined the in vivo mutagenicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) by using transgenic (Tg) zebrafish carrying the mutational target gene rpsL. PBTA-6 is one of the PBTA-type compounds that were recently identified in highly mutagenic river water in Japan. BaP is a well-known contaminant that is frequently found in polluted water. Both compounds are potent mutagens, as determined by using the Ames test employing S9 mix and Salmonella. Adult rpsL Tg zebrafish were exposed to 0, 7, or 10 mg/L PBTA-6 or 0, 1.5, or 3 mg/L BaP for 96 h in a water bath and the mutations in their gills and hepatopancreata were measured 2-4 weeks later. At 3 weeks after exposure, 3 mg/L BaP significantly increased the rpsL mutant frequency (MF) in the gill and hepatopancreas by 5- and 2.3-fold, respectively, as compared to control fish. Sequence analysis showed that BaP mainly induced G:C to T:A and G:C to C:G transversions, which is consistent with the known mutagenic effects of BaP. In contrast, despite its extremely high mutagenic potency in Salmonella strains, PBTA-6 did not significantly increase the MF in the zebrafish gill or hepatopancreas. Although PBTA-6 is 300 times more mutagenic than BaP in the Ames test [T. Watanabe, H. Nukaya, Y. Terao, Y. Takahashi, A. Tada, T. Takamura, H. Sawanishi, T. Ohe, T. Hirayama, T. Sugimura, K. Wakabayashi, Synthesis of 2-phenylbenzotriazole-type mutagens, PBTA-5 and PBTA-6, and their detection in river water from Japan, Mutat. Res. 498 (2001) 107-115], calculation of the mutagenicity per mole of compound indicated that PBTA-6 was 33- and BaP.

  9. Course transformation: Content, structure and effectiveness analysis

    DuHadway, Linda P.

    The organization of learning materials is often limited by the systems available for delivery of such material. Currently, the learning management system (LMS) is widely used to distribute course materials. These systems deliver the material in a text-based, linear way. As online education continues to expand and educators seek to increase their effectiveness by adding more effective active learning strategies, these delivery methods become a limitation. This work demonstrates the possibility of presenting course materials in a graphical way that expresses important relations and provides support for manipulating the order of those materials. The ENABLE system gathers data from an existing course, uses text analysis techniques, graph theory, graph transformation, and a user interface to create and present graphical course maps. These course maps are able to express information not currently available in the LMS. Student agents have been developed to traverse these course maps to identify the variety of possible paths through the material. The temporal relations imposed by the current course delivery methods have been replaced by prerequisite relations that express ordering that provides educational value. Reducing the connections to these more meaningful relations allows more possibilities for change. Technical methods are used to explore and calibrate linear and nonlinear models of learning. These methods are used to track mastery of learning material and identify relative difficulty values. Several probability models are developed and used to demonstrate that data from existing, temporally based courses can be used to make predictions about student success in courses using the same material but organized without the temporal limitations. Combined, these demonstrate the possibility of tools and techniques that can support the implementation of a graphical course map that allows varied paths and provides an enriched, more informative interface between the educator


    赵晓兵; 薛大为; 赵玉祥


    In selecting rational types of underground structures resisting explosion, in order to improve stress states of the structural section and make full use of material strength of each part of the section, the research method of composite structures is presented. Adopting the analysis method of micro-section free body, equilibrium equations, constraint equations and deformation coordination equations are given. Making use of the concept of generalized work and directly introducing Lagrange multiplier specific in physical meaning, the validity of the constructed generalized functional is proved by using variation method. The rational rigidity matching relationship of composite structure section is presented through example calculations.

  11. Application Analysis of Strengthened Story in Frame-Core Structures

    SU Yuan; CHEN Chuan-yao; LI Li


    Lateral deflection formulas are presented for analysis of the strengthened story applied to frame-core structures. For the frame-core structures with top outriggers and with middle outriggers, the relationship between stiffness characteristic parameters of frame and outriggers and the top drift of structures under different loads is analyzed. It is indicated that when stiffness characteristic parameter of frame is large, outrigger efficiency for top drift reduction is low, and the mutation of internal forces occurs; when the stiffness characteristic parameter of frame is less than 3, installing the strengthened story is advantageous to frame-core structures.

  12. A discourse on sensitivity analysis for discretely-modeled structures

    Adelman, Howard M.; Haftka, Raphael T.


    A descriptive review is presented of the most recent methods for performing sensitivity analysis of the structural behavior of discretely-modeled systems. The methods are generally but not exclusively aimed at finite element modeled structures. Topics included are: selections of finite difference step sizes; special consideration for finite difference sensitivity of iteratively-solved response problems; first and second derivatives of static structural response; sensitivity of stresses; nonlinear static response sensitivity; eigenvalue and eigenvector sensitivities for both distinct and repeated eigenvalues; and sensitivity of transient response for both linear and nonlinear structural response.

  13. Accuracy of Dynamic and Acoustic Analysis of Lightweight Panel Structures

    Kirkegaard, Poul Henning; Dickow, Kristoffer Ahrens; Andersen, Lars Vabbersgaard


    in such buildings is important. In the lowfrequency range, prediction of sound and vibration in building structures may be achieved by finite-element analysis (FEA). The aim of this paper is to compare the two commercial codes ABAQUS and ANSYS for FEA of an acoustic-structural coupling in a timber lightweight panel...... structure. For this purpose, modal analyses are carried out employing a fully coupled model of sound waves within an acoustic medium and vibrations in the structural part. The study concerns the frequency range 50–250 Hz....

  14. Modeling and structural analysis of PA clan serine proteases

    Laskar Aparna


    Full Text Available Abstract Background Serine proteases account for over a third of all known proteolytic enzymes; they are involved in a variety of physiological processes and are classified into clans sharing structural homology. The PA clan of endopeptidases is the most abundant and over two thirds of this clan is comprised of the S1 family of serine proteases, which bear the archetypal trypsin fold and have a catalytic triad in the order Histidine, Aspartate, Serine. These proteases have been studied in depth and many three dimensional structures have been experimentally determined. However, these structures mostly consist of bacterial and animal proteases, with a small number of plant and fungal proteases and as yet no structures have been determined for protozoa or archaea. The core structure and active site geometry of these proteases is of interest for many applications. This study investigated the structural properties of different S1 family serine proteases from a diverse range of taxa using molecular modeling techniques. Results Our predicted models from protozoa, archaea, fungi and plants were combined with the experimentally determined structures of 16 S1 family members and used for analysis of the catalytic core. Amino acid sequences were submitted to SWISS-MODEL for homology-based structure prediction or the LOOPP server for threading-based structure prediction. Predicted models were refined using INSIGHT II and SCRWL and validated against experimental structures. Investigation of secondary structures and electrostatic surface potential was performed using MOLMOL. The structural geometry of the catalytic core shows clear deviations between taxa, but the relative positions of the catalytic triad residues were conserved. Some highly conserved residues potentially contributing to the stability of the structural core were identified. Evolutionary divergence was also exhibited by large variation in secondary structure features outside the core

  15. Structural Analysis: Shape Information via Points-To Computation

    Marron, Mark


    This paper introduces a new hybrid memory analysis, Structural Analysis, which combines an expressive shape analysis style abstract domain with efficient and simple points-to style transfer functions. Using data from empirical studies on the runtime heap structures and the programmatic idioms used in modern object-oriented languages we construct a heap analysis with the following characteristics: (1) it can express a rich set of structural, shape, and sharing properties which are not provided by a classic points-to analysis and that are useful for optimization and error detection applications (2) it uses efficient, weakly-updating, set-based transfer functions which enable the analysis to be more robust and scalable than a shape analysis and (3) it can be used as the basis for a scalable interprocedural analysis that produces precise results in practice. The analysis has been implemented for .Net bytecode and using this implementation we evaluate both the runtime cost and the precision of the results on a num...

  16. Dynamic Analysis of Partially Embedded Structures Considering Soil-Structure Interaction in Time Domain

    Sanaz Mahmoudpour


    Full Text Available Analysis and design of structures subjected to arbitrary dynamic loadings especially earthquakes have been studied during past decades. In practice, the effects of soil-structure interaction on the dynamic response of structures are usually neglected. In this study, the effect of soil-structure interaction on the dynamic response of structures has been examined. The substructure method using dynamic stiffness of soil is used to analyze soil-structure system. A coupled model based on finite element method and scaled boundary finite element method is applied. Finite element method is used to analyze the structure, and scaled boundary finite element method is applied in the analysis of unbounded soil region. Due to analytical solution in the radial direction, the radiation condition is satisfied exactly. The material behavior of soil and structure is assumed to be linear. The soil region is considered as a homogeneous half-space. The analysis is performed in time domain. A computer program is prepared to analyze the soil-structure system. Comparing the results with those in literature shows the exactness and competency of the proposed method.

  17. Dynamic Analysis of Composite Structural System for Looms Industry

    Jigar K. Sevalia


    Full Text Available All the structures subjected to any kind of loads or displacement tends to behave dynamically. Thus the structures are always under continuous loading. The industrial buildings have to support the machineries in motion which are under high degree of vibrations. And so the design of base and the foundations of such structures under vibrations are very important and need to be stable. Problems of dynamics of bases and foundations are to be studied carefully, so as to understand the response characteristics of the power loom industry structure. This is very important from the economic point of view as well as to secure the stability and safety of the structure; dynamic analysis was carried out for Ground + One storey industry load bearing structure using STAAD.Pro software. In this paper, an attempt has been made to study the dynamic analysis of the structure under vibrations caused by reciprocating type machines. This paper makes attempt to study the effects of various structural parameters like Beam Size, Column Size and Storey Height and Wall Thickness variation on Frequency and Displacement of the industrial building which in future will serve as guidelines to the structural engineers and the industry people.

  18. Ambient Response Analysis Modal Analysis for Large Structures

    Brincker, Rune; Andersen, Palle


    In this paper an outline is given of the basic ideas in ambient respons analysis, i.e. when modal analyses is performed on systems based on output only. Some of the most known techniques are briefly introduced, and the basic problems discussed. The introduced techniques are the frequency domain...... based peak-picking methods, the polyreference LSCE method, the stochastic subspace method for estimation of state space systems and the prediction error method for estimation of Auto-Regressive Moving Average Vector models. The techniques are illustrated on an example of ambient response measurments...

  19. RNA STRAND: The RNA Secondary Structure and Statistical Analysis Database

    Andronescu Mirela


    Full Text Available Abstract Background The ability to access, search and analyse secondary structures of a large set of known RNA molecules is very important for deriving improved RNA energy models, for evaluating computational predictions of RNA secondary structures and for a better understanding of RNA folding. Currently there is no database that can easily provide these capabilities for almost all RNA molecules with known secondary structures. Results In this paper we describe RNA STRAND – the RNA secondary STRucture and statistical ANalysis Database, a curated database containing known secondary structures of any type and organism. Our new database provides a wide collection of known RNA secondary structures drawn from public databases, searchable and downloadable in a common format. Comprehensive statistical information on the secondary structures in our database is provided using the RNA Secondary Structure Analyser, a new tool we have developed to analyse RNA secondary structures. The information thus obtained is valuable for understanding to which extent and with which probability certain structural motifs can appear. We outline several ways in which the data provided in RNA STRAND can facilitate research on RNA structure, including the improvement of RNA energy models and evaluation of secondary structure prediction programs. In order to keep up-to-date with new RNA secondary structure experiments, we offer the necessary tools to add solved RNA secondary structures to our database and invite researchers to contribute to RNA STRAND. Conclusion RNA STRAND is a carefully assembled database of trusted RNA secondary structures, with easy on-line tools for searching, analyzing and downloading user selected entries, and is publicly available at

  20. RF-thermal-structural-RF coupled analysis on a travelling wave disk-loaded accelerating structure

    PEI Shi-Lun; CHI Yun-Long; ZHANG Jing-Ru; HOU Mi; LI Xiao-Ping


    The travelling wave (TW) disk-loaded accelerating structure is one of the key components in normal conducting (NC) linear accelerators,and has been studied for many years.In the design process,usually after the dimensions of each cell and the two couplers are finalized,the structure is fabricated and tuned,and then the whole structure RF characteristics are measured by using a vector network analyzer.Before fabrication,the whole structure characteristics (including RF,thermal and structural ones) are less simulated due to the limited capability of currently available computers.In this paper,we described a method for performing RF-thermal-structural-RF coupled analysis on a TW disk-loaded structure using only one PC.In order to validate our method,we first analyzed and compared our RF simulation results on the 3 m long BEPC Ⅱ structure with the corresponding experimental results,which shows very good consistency.Finally,the RF-thermal-structure-RF coupled analysis results on the 1.35 m long NSC KIPT linac accelerating structure are presented.

  1. Structural analysis of guyed mast exposed to wind action

    Pezo Milada L.


    Full Text Available The behavior of the mast is non-linear due to its slenderness and compliant guy-support system, having a tendency to lose stability and even crush suddenly. Wind load is one of the main factors affecting the stability of the structure of the mast. Structural assessment of the different mast configurations has been investigated in the past. Furthermore, European standards EN 1993-3-1:2006 and EN 1993-1-6:2007 already provides some guidelines about the basis of structural analysis of masts and towers. This paper presents the results of numerical simulations of a guyed mast exposed to wind action using finite element method. Structural analyses were performed for three different constant wind loads, modal analysis provides the values of natural frequency and mode shapes, while the stability analysis was performed for the first three buckling load factor values. The motivation for this study is to investigate the contribution of finite element method to structural analysis of a lattice structure such as guyed mast as an alternative and/or improvement to the literature and codes. [Projekat Ministarstva nauke Republike Srbije, br. TR33036

  2. Numerical Analysis of Vibrations of Structures under Moving Inertial Load

    Bajer, Czeslaw I


    Moving inertial loads are applied to structures in civil engineering, robotics, and mechanical engineering. Some fundamental books exist, as well as thousands of research papers. Well known is the book by L. Frýba, Vibrations of Solids and Structures Under Moving Loads, which describes almost all problems concerning non-inertial loads. This book presents broad description of numerical tools successfully applied to structural dynamic analysis. Physically we deal with non-conservative systems. The discrete approach formulated with the use of the classical finite element method results in elemental matrices, which can be directly added to global structure matrices. A more general approach is carried out with the space-time finite element method. In such a case, a trajectory of the moving concentrated parameter in space and time can be simply defined. We consider structures described by pure hyperbolic differential equations such as strings and structures described by hyperbolic-parabolic differential equations ...

  3. Purification and Structural Analysis of Plectin and BPAG1e.

    Manso, José A; García Rubio, Inés; Gómez-Hernández, María; Ortega, Esther; Buey, Rubén M; Carballido, Ana M; Carabias, Arturo; Alonso-García, Noelia; de Pereda, José M


    Plectin and BPAG1e belong to the plakin family of high-molecular-weight proteins that interconnect the cytoskeletal systems and anchor them to junctional complexes. Plectin and BPAG1e are prototypical plakins with a similar tripartite modular structure. The N- and C-terminal regions are built of multiple discrete structural domains, while the central rod domain mediates dimerization by coiled-coil interactions. Owing to the mosaic organization of plakins, the structure of their constituent individual domains or small multi-domain segments can be analyzed isolated. Yet, understanding the integrated function of large regions, oligomers, and heterocomplexes of plakins is difficult due to the large and segmented structure. Here, we describe methods for the production of plectin and BPAG1e samples suitable for structural and biophysical analysis. In addition, we discuss the combination of hybrid methods that yield information at several resolution levels to study the complex, multi-domain, and flexible structure of plakins.

  4. Numerical Analysis on Neutron Shielding Structure of ITER Vacuum Vessel

    LIU Changle; WU Songtao; YU Jie; SHENG Daolin


    The neutron shielding component of ITER (International Thermonuclear Experi-mental Reactor) vacuum vessel is a kind of structure resembling a wall in appearance. A FE (finite element) model is set up by using ANSYS code in terms of its structural features. Static analysis, thermal expansion analysis and dynamic analysis are performed. The static results show that the stress and displacement distribution are allowable, but the high stress appears in the junction between the upper and lower parts. The modal analysis indicates that the biggest defor-mation exists in the port area. Through modal superposition, the single-point response has been found with the lower rank frequency of the acceleration seismic response spectrum. But the defor-mation and the stress values are within the permissible limit. The analysis results would benefit the work in the next step and provide some reference for the implementation of the engineering plan in the future.

  5. Syntheses and structural characterization of new dithiophosphinato cadmium complexes

    Ertuğrul Gazi̇ Sağlam; Nurcan Acar; Yasemi̇n Süzen; Berline Mougang-Soumé; Tuncer Hökelek


    New cadmium complexes of 4-methoxyphenyl dithiophosphinic acids, H3CO-C6H4-(R)PS2H were prepared. The five dithiophosphinato ligands (L) involved were of the general structure H3CO-C6H4-(R)PS2−with R= 3-methylbutyl, (L1); n-butyl, (L2); 2-methylpropyl, (L3); 1-methylpropyl, (L4) and 2-propyl, (L5).To the best of our knowledge, this is the first report on the preparation and characterization of the n-butyl-derivative. The acid forms of the ligands were obtained by treatment of the Lawesson reagent, (LR) [2,4-bis(4-methoxyphenyl)-1,3,2,4-dithiadiphosphetane-2,4-disulfide] with the corresponding Grignard reagent in dry diethylether. The acids formed were transformed into easily crystallizable ammonium salts (NH4L) for purification. These salts were treated with CdCl2 in ethanol at room temperature to produce the bis-dithiophosphinato cadmium complexes ([Cd(L)2]2) exclusively. The structures of the complexes were elucidated by elemental analysis, MS, FTIR and Raman spectroscopy techniques as well as 1H-, 13C- and 31P- NMR. The crystal structures of [Cd(L1)2]2 and [Cd(L2)2]2 were also studied as examples. X-ray studies confirmed the nonplanar, four-coordination geometry of the complexes and indicate that electron delocalization prevails in the PS$_{−}^{2}$ moiety of the dithiophosphinato groups.

  6. Is it Worth it? A Comparative Analysis of Cost-Benefit Projectionsfor State Renewables Portfolio Standards

    Chen, Cliff; Wiser, Ryan; Bolinger, Mark


    State renewables portfolio standards (RPS) have emerged as one of the most important policy drivers of renewable energy capacity expansion in the U.S. Collectively, these policies now apply to almost 40% of U.S. electricity load, and may have substantial impacts on electricity markets, ratepayers, and local economies. As RPS policies have been proposed or adopted in an increasing number of states, a growing number of studies have attempted to quantify the potential impacts of these policies, focusing primarily on projecting cost impacts, but sometimes also estimating macroeconomic and environmental effects. This report synthesizes and analyzes the results and methodologies of twenty-six distinct state or utility-level RPS cost impact analyses completed since 1998 (see Figure 1 and Appendix for a complete list of the studies). Together, these studies model proposed or adopted RPS policies in seventeen different states. We highlight the key findings of these studies on the costs and benefits of RPS policies, examine the sensitivity of projected costs to model assumptions, assess the attributes of different modeling approaches, and suggest possible areas of improvement for future RPS analysis.

  7. FMRFamide-related peptides (FaRPs): A new family of peptides from amphibian defensive skin secretions

    Wang, Lei; Smyth, Anita; Johnsen, Anders


    was cloned from a skin secretion library and found to contain a single copy of the peptide located at the C-terminus of a 58 amino acid residue open-reading frame. These data extend the potential targets of the defensive arsenal of amphibian tegumental secretions to parasitic/predatory invertebrates......Amphibian defensive skin secretions are known to contain a plethora of biologically-active peptides that are often structural and functional analogues of vertebrate neuropeptides. Here we report the structures of two invertebrate neuropeptide analogues, IPPQFMRF amide (IF-8 amide) and EGDEDEFLRF...

  8. Skeleton-Sectional Structural Analysis for 3D Printing

    Wen-Peng Xu; Wei Li; Li-Gang Liu


    3D printing has become popular and has been widely used in various applications in recent years. More and more home users have motivation to design their own models and then fabricate them using 3D printers. However, the printed objects may have some structural or stress defects as the users may be lack of knowledge on stress analysis on 3D models. In this paper, we present an approach to help users analyze a model’s structural strength while designing its shape. We adopt sectional structural analysis instead of conventional FEM (Finite Element Method) analysis which is computationally expensive. Based on sectional structural analysis, our approach imports skeletons to assist in integrating mesh designing, strength computing and mesh correction well. Skeletons can also guide sections building and load calculation for analysis. For weak regions with high stress over a threshold value in the model from analysis result, our system corrects them by scaling the corresponding bones of skeleton so as to make these regions stiff enough. A number of experiments have demonstrated the applicability and practicability of our approach.

  9. Metalloprotease NleC suppresses host NF-κB/inflammatory responses by cleaving p65 and interfering with the p65/RPS3 interaction.

    Andrea Hodgson


    Full Text Available Attaching/Effacing (A/E pathogens including enteropathogenic Escherichia coli (EPEC, enterohemorrhagic E. coli (EHEC and the rodent equivalent Citrobacter rodentium are important causative agents of foodborne diseases. Upon infection, a myriad of virulence proteins (effectors encoded by A/E pathogens are injected through their conserved type III secretion systems (T3SS into host cells where they interfere with cell signaling cascades, in particular the nuclear factor kappaB (NF-κB signaling pathway that orchestrates both innate and adaptive immune responses for host defense. Among the T3SS-secreted non-LEE-encoded (Nle effectors, NleC, a metalloprotease, has been recently elucidated to modulate host NF-κB signaling by cleaving NF-κB Rel subunits. However, it remains elusive how NleC recognizes NF-κB Rel subunits and how the NleC-mediated cleavage impacts on host immune responses in infected cells and animals. In this study, we show that NleC specifically targets p65/RelA through an interaction with a unique N-terminal sequence in p65. NleC cleaves p65 in intestinal epithelial cells, albeit a small percentage of the molecule, to generate the p65¹⁻³⁸ fragment during C. rodentium infection in cultured cells. Moreover, the NleC-mediated p65 cleavage substantially affects the expression of a subset of NF-κB target genes encoding proinflammatory cytokines/chemokines, immune cell infiltration in the colon, and tissue injury in C. rodentium-infected mice. Mechanistically, the NleC cleavage-generated p65¹⁻³⁸ fragment interferes with the interaction between p65 and ribosomal protein S3 (RPS3, a 'specifier' subunit of NF-κB that confers a subset of proinflammatory gene transcription, which amplifies the effect of cleaving only a small percentage of p65 to modulate NF-κB-mediated gene expression. Thus, our results reveal a novel mechanism for A/E pathogens to specifically block NF-κB signaling and inflammatory responses by cleaving a

  10. Research on Morphing Wing with 3-RPS Parallel Mechanism%基于3-RPS并联机构的自适应机翼方法实现

    田鑫; 王帮峰; 吴佳俊


    针对自适应机翼飞行器设计了一种翼身连动机构,使机翼能够完成变前后掠、变翼展、变上下反角三种变形,从而在整个飞行任务过程中都能使飞行器达到气动性能最优.该连动机构以经典3-RPS并联机构为原型,通过3杆联动完成机翼姿态的变化.根据自适应机翼的变形要求,该结构对经典3-RPS机构进行改进,使机构在单一转动自由度和平动自由度的复合运动中,不会产生衍生自由度.且新结构截面外形与机翼截面的几何构型更为接近,较之经典3-RPS机构更适合应用于自适应机翼的连动机构.此外,电控系统设计也更利于系统未来优化升级.%A linkage mechanism was designed for morphing wing, making it possible for the variation of sweep,dihedral angle and wingspan. And thus the optimum aerodynamic performance can be achieved for the aircraft during the whole flight missions. Based on classic 3-RPS parallel mechanism, the design can alter the attitude of the morphing wing by tri-bar linkage. In order to meet the deformation need of morphing wing, the improvement was made for the classic 3-RPS parallel mechanism so that no derivative freedom was induced during the combined motion of single rotational freedom and translational freedom. The new mechanism fits the linkage of morphing wing better as its cross-section shape is more similar to the geometry of the airfoil section compared to the classic one. Moreover, the design of electric control system makes it possible for future optimization and upgrading.

  11. Transfinite element methodology towards a unified thermal/structural analysis

    Tamma, K. K.; Railkar, S. B.


    The paper describes computational developments towards thermal/structural modeling and analysis via a generalized common numerical methodology for effectively and efficiently interfacing interdisciplinary areas. The proposed formulations use transform methods in conjunction with finite element developments for each of the heat transfer and structural disciplines, respectively, providing avenues for obtaining the structural response due to thermal effects. An alternative methodology for unified thermal/structural analysis is presented. The potential of the approach is outlined in comparison with conventional schemes and existing practices. Highlights and characteristic features of the approach are described via general formulations and applications to several problems. Results obtained demonstrate excellent agreement in comparison with analytic and/or conventional finite element schemes accurately and efficiently.

  12. Structural Analysis of the ITER VV Lower Port Region

    CAI Yingxiang; WU Songtao; YU Jie


    A structural analysis of the International Thermonuclear Experimental Reactor (ITER)vacuum vessel's lower port region Was presented by means of a finite element analysis method.The purpose is to evaluate the stress and displacement level on this structure under various combinations of five designed loads,including the gravity of the vacuum vessel,seismic loads,electromagnetic loads,and possible pressure loads to ensure structural safety.The cyclic symmetry finite element model of this structure Was developed by using ANSYS code.The results showed that the maximum stress does not exceed the allowable value for any of the load combinations according to ASME code and the nine vacuum vessel(VV)supports have the ability to sustain the entire VV and in vessel-components and withstand load combinations under both normal as well as off-normal operation conditions.Stress mainly concentrates on the connecting region of the VV support and lower port stub extension.

  13. A Morphogenetic Design Approach with Embedded Structural Analysis

    Jensen, Mads Brath; Kirkegaard, Poul Henning; Holst, Malene Kirstine


    The present paper explores a morphogenetic design approach with embedded structural analysis for architectural design. A material system based on a combined space truss and membrane system has been derived as a growth system with inspiration from natural growth of plants. The structural system...... is capable of adding new elements based on a structural analysis of the existing components and their internal stress levels. A GA decision-making procedure that control the generation of the growth cycles is introduced. This evaluation and generation loop is capable of successfully making decisions based...... on several, and often conflicting, inputs formulated from architectural requirements. An experiment with a tri-pyramid component has been considered, but many other space truss systems could be explored in the same manner and result in highly performative outcomes. not only with respect to the structural...

  14. Earthquake resistant structural design of house JUBHome using simplified analysis

    Lenarčič, Matej


    In this thesis we have designed steel reinforcement for a structure using JUBhome system, on the basis of simplified analysis. Design of the structure was carried out according to standards and guidelines for earthquake resistant design of foundations and reinforced concrete walls. In the first part of thesis we have introduced earthquake loads, guidelines for earthquake resistant design and controls, which have to be fulfilled, when using foundation system JUBHome BASE and wall JUBHome WALL....

  15. Structural Analysis of Bacillus subtilis Spore Peptidoglycan During Sporulation


    Structural analysis of Bacillus subtilis spore peptidoglycan during sporulation:Jennifer L. Meador-Parton:David L. Popham, Chairman:Department of Biology:(ABSTRACT):Bacterial spore peptidoglycan (PG) is very loosely cross-linked relative to vegetative PG. Theories suggest that loosely cross-linked spore PG may have a flexibility which contributes to the attainment of spore core dehydration. The structure of the PG found in fully dormant spores has previously been examined in wild type and m...

  16. Protein secondary structure analysis with a coarse-grained model

    Kneller, Gerald R.; Hinsen, Konrad


    The paper presents a geometrical model for protein secondary structure analysis which uses only the positions of the $C_{\\alpha}$-atoms. We construct a space curve connecting these positions by piecewise polynomial interpolation and describe the folding of the protein backbone by a succession of screw motions linking the Frenet frames at consecutive $C_{\\alpha}$-positions. Using the ASTRAL subset of the SCOPe data base of protein structures, we derive thresholds for the screw parameters of se...

  17. An Approach to Structural Approximation Analysis by Artificial Neural Networks

    陆金桂; 周济; 王浩; 陈新度; 余俊; 肖世德


    This paper theoretically proves that a three-layer neural network can be applied to implementing exactly the function between the stresses and displacements and the design variables of any elastic structure based on the Kolmogorov’s mapping neural network existence theorem. A new approach to the structural approximation analysis with the global characteristic based on artificial neural networks is presented. The computer simulation experiments made by this paper show that the new approach is effective.

  18. Efficient explicit formulation for practical fuzzy structural analysis

    A S Balu; B N Rao


    This paper presents a practical approach based on High Dimensional Model Representation (HDMR) for analysing the response of structures with fuzzy parameters. The proposed methodology involves integrated finite element modelling, HDMR based response surface generation, and explicit fuzzy analysis procedures. The uncertainties in the material, geometric, loading and structural parameters are represented using fuzzy sets. To facilitate efficient computation, a HDMR based response surface generation is employed for the approximation of the fuzzy finite element response quantity.

  19. Better Finite-Element Analysis of Composite Shell Structures

    Clarke, Gregory


    A computer program implements a finite-element-based method of predicting the deformations of thin aerospace structures made of isotropic materials or anisotropic fiber-reinforced composite materials. The technique and corresponding software are applicable to thin shell structures in general and are particularly useful for analysis of thin beamlike members having open cross-sections (e.g. I-beams and C-channels) in which significant warping can occur.

  20. Identification of similar regions of protein structures using integrated sequence and structure analysis tools

    Heiland Randy


    Full Text Available Abstract Background Understanding protein function from its structure is a challenging problem. Sequence based approaches for finding homology have broad use for annotation of both structure and function. 3D structural information of protein domains and their interactions provide a complementary view to structure function relationships to sequence information. We have developed a web site and an API of web services that enables users to submit protein structures and identify statistically significant neighbors and the underlying structural environments that make that match using a suite of sequence and structure analysis tools. To do this, we have integrated S-BLEST, PSI-BLAST and HMMer based superfamily predictions to give a unique integrated view to prediction of SCOP superfamilies, EC number, and GO term, as well as identification of the protein structural environments that are associated with that prediction. Additionally, we have extended UCSF Chimera and PyMOL to support our web services, so that users can characterize their own proteins of interest. Results Users are able to submit their own queries or use a structure already in the PDB. Currently the databases that a user can query include the popular structural datasets ASTRAL 40 v1.69, ASTRAL 95 v1.69, CLUSTER50, CLUSTER70 and CLUSTER90 and PDBSELECT25. The results can be downloaded directly from the site and include function prediction, analysis of the most conserved environments and automated annotation of query proteins. These results reflect both the hits found with PSI-BLAST, HMMer and with S-BLEST. We have evaluated how well annotation transfer can be performed on SCOP ID's, Gene Ontology (GO ID's and EC Numbers. The method is very efficient and totally automated, generally taking around fifteen minutes for a 400 residue protein. Conclusion With structural genomics initiatives determining structures with little, if any, functional characterization

  1. Analysis of gallstone composition and structure in Jharkhand region.

    Sharma, Rajani; Soy, Snehi; Kumar, Chandan; Sachan, Shashwati Ghosh; Sharma, Shubha Rani


    The aim of this study was to analyze gallstones structurally and chemically as this may help to direct the measures for its treatment. On the basis of morphology, 459 gallstones were categorized into pigmented, cholesterol, and mixed gallstones and analyzed for their chemical structure and composition. Elementary analysis was done with the help of inductively coupled plasma, optical emission spectrophotometry. Fourier transform infrared spectroscopy was used for compound analysis. The effects of thermal stability were analyzed by thermal gravimetric analysis (TGA). Statistical analysis was done to correlate gallstone composition with their shape and number in gallbladder. Pigmented gallstones were predominant and generally occurred in multiple forms, cholesterol as solitaire and black pigmented as slug (χ (2) = 3.56; p gallstones while pigmented gallstones were more compact in structure. Both types of structure were seen in mixed stones while black pigmented stones were amorphous in nature. This difference in structures might be due to difference in chemical compositions. Cholesterol and mixed stones contained basically cholesterol, brown pigmented constituted bilirubin as a major component, and black stones differed from brown pigmented stones by the presence of Ca palmitate. Bilirubin and palmitate were thermally more stable than cholesterol; hence pigmented gallstones were thermally more stable than cholesterol gallstones, as seen in TGA study. Pigmented gallstones were most common in this study of gallstones from the Jharkhand region.

  2. Reliability analysis of ship structure system with multi-defects


    This paper analyzes the influence of multi-defects including the initial distortions,welding residual stresses,cracks and local dents on the ultimate strength of the plate element,and has worked out expressions of reliability calculation and sensitivity analysis of the plate element.Reliability analysis is made for the system with multi-defects plate elements.Failure mechanism,failure paths and the calculating approach to global reliability index are also worked out.After plate elements with multi-defects fail,the formula of reverse node forces which affect the residual structure is deduced,so are the sensitivity expressions of the system reliability index.This ensures calculating accuracy and rationality for reliability analysis,and makes it convenient to find weakness plate elements which affect the reliability of the structure system.Finally,for the validity of the approach proposed,we take the numerical example of a ship cabin to compare and contrast the reliability and the sensitivity analysis of the structure system with multi-defects with those of the structure system with no defects.The approach has implications for the structure design,rational maintenance and renewing strategy.

  3. Finite element analysis of structures through unified formulation

    Carrera, Erasmo; Petrolo, Marco; Zappino, Enrico


    The finite element method (FEM) is a computational tool widely used to design and analyse  complex structures. Currently, there are a number of different approaches to analysis using the FEM that vary according to the type of structure being analysed: beams and plates may use 1D or 2D approaches, shells and solids 2D or 3D approaches, and methods that work for one structure are typically not optimized to work for another. Finite Element Analysis of Structures Through Unified Formulation deals with the FEM used for the analysis of the mechanics of structures in the case of linear elasticity. The novelty of this book is that the finite elements (FEs) are formulated on the basis of a class of theories of structures known as the Carrera Unified Formulation (CUF). It formulates 1D, 2D and 3D FEs on the basis of the same ''fundamental nucleus'' that comes from geometrical relations and Hooke''s law, and presents both 1D and 2D refined FEs that only have displacement variables as in 3D elements. It also covers 1D...

  4. Tools for integrated sequence-structure analysis with UCSF Chimera

    Huang Conrad C


    Full Text Available Abstract Background Comparing related structures and viewing the structures in the context of sequence alignments are important tasks in protein structure-function research. While many programs exist for individual aspects of such work, there is a need for interactive visualization tools that: (a provide a deep integration of sequence and structure, far beyond mapping where a sequence region falls in the structure and vice versa; (b facilitate changing data of one type based on the other (for example, using only sequence-conserved residues to match structures, or adjusting a sequence alignment based on spatial fit; (c can be used with a researcher's own data, including arbitrary sequence alignments and annotations, closely or distantly related sets of proteins, etc.; and (d interoperate with each other and with a full complement of molecular graphics features. We describe enhancements to UCSF Chimera to achieve these goals. Results The molecular graphics program UCSF Chimera includes a suite of tools for interactive analyses of sequences and structures. Structures automatically associate with sequences in imported alignments, allowing many kinds of crosstalk. A novel method is provided to superimpose structures in the absence of a pre-existing sequence alignment. The method uses both sequence and secondary structure, and can match even structures with very low sequence identity. Another tool constructs structure-based sequence alignments from superpositions of two or more proteins. Chimera is designed to be extensible, and mechanisms for incorporating user-specific data without Chimera code development are also provided. Conclusion The tools described here apply to many problems involving comparison and analysis of protein structures and their sequences. Chimera includes complete documentation and is intended for use by a wide range of scientists, not just those in the computational disciplines. UCSF Chimera is free for non-commercial use and is

  5. 2-D Finite Element Analysis of Massive RC Structures

    Saabye Ottosen, Niels


    Nonlinear analysis of concrete structures using finite elements is discussed. The applications include a thick-walled top-closure for a pressure vessel as well as the delicate problems of beams failing in shear. The top-closure analysis evaluates the effect of two different failure criteria...... and modeling of a realistic post-failure behavior is demonstrated to be mandatory for accurate structural predictions. For shear beams it is shown that the primary cause of failure is strain softening adjacent to the load point....


    ZhaoYan; LinJiahao; ZhangYahui; AnWei


    A nonlinear seismic analysis method for complex frame structures subjected to stationary random ground excitations is proposed. The nonlinear elasto-plastic behaviors may take place only on a small part of the structure. The Bouc-Wen differential equation model is used to model the hysteretic characteristics of the nonlinear components. The Pseudo Excitation Method (PEM) is used in solving the linearized random differential equations to replace the solution of the less efficient Lyapunov equation. Numerical results of a real bridge show that .the method proposed is effective for practical engineering analysis.

  7. Analysis of structural hot-spot stress in orthotropic plates

    Chen Ce; Ji Bohai; Xu Hanjiang


    On the basis of the actual steel deck structure of Taizhou Bridge, this paper carries out hot-spot stress analysis on some key spots by using the finite element model which simulates local structure of orthotropic steel bridge decks. A finite element model is established for local structure of orthotropic steel bridge decks, and in the analysis of linear elasticity of the structure, face load is employed to simulate the loads from vehicle wheels. Analysis results show that main stresses are relatively heavy at the joints between diaphragm plates, top plates and U-shaped ribs and the joints between diaphragm plates and U-shaped ribs. These joints shall be regarded as key points for hot-spot stress analysis. Different mesh densities are adopted in the finite element model and the main stresses at different hot spots are contrasted and linear extrapolation is carried out using extrapolation formulae. Results show that different mesh densities have different influences on the hot-spot stresses at the welded seams of U-shaped ribs. These influences shall be considered in calculation and analysis.

  8. Optimal fatigue analysis of structures during complex loadings

    Karaouni Habib


    Full Text Available A new framework for high cycle fatigue analysis of metallic structures under complex multi-parameter loadings was here developed. This allows to reduce the analysis on a 2-D window with a characterized one-parameter cyclic loading thanks to an equivalence rule relative to damage between any two loadings. The simplified inelastic analysis introduced by J. Zarka [J. Zarka et al. 1990. A new approach in inelastic analysis of structures. CADLM] was used to find the limit state of the structure. A new design rules for fatigue analysis by utilizing automatic learning systems was successfully performed. A database was built by coupling numerical simulations and experimental results on several welded specimens which are considered as a general structure in the proposed approach. This could be possible by the introduction of an intelligent description of a general fatigue case based on the actual theories and models. A software, FATPRO [M.I. Systems, FatPro, available at], based on this work has been developed at MZ Intelligent Systems.

  9. Random Time Dependent Resistance Analysis on Reinforced Concrete Structures

    GUAN Chang-sheng; WU Ling


    The analysis method on random time dependence of reinforced concrete material is introduced,the effect mechanism on reinforced concrete are discussed, and the random time dependence resistance of reinforced concrete is studied. Furthermore, the corrosion of steel bar in reinforced concrete structures is analyzed. A practical statistical method of evaluating the random time dependent resistance, which includes material, structural size and calculation influence, is also established. In addition, an example of predicting random time dependent resistance of reinforced concrete structural element is given.

  10. Analysis of the spiral structure in a simulated galaxy

    Mata-Chávez, Dolores; Puerari, Ivânio


    We analyze the spiral structure that results in a numerical simulation of a galactic disk with stellar and gaseous components evolving in a potential that includes an axisymmetric halo and bulge. We perform a second simulation without the gas component to observe how it affects the spiral structure in the disk. To quantify this, we use a Fourier analysis and obtain values for the pitch angle and the velocity of the self-excited spiral pattern of the disk. The results show a tighter spiral in the simulation with gaseous component. The spiral structure is consistent with a superposition of waves, each with a constant pattern velocity in given radial ranges.

  11. Structural parameter identifiability analysis for dynamic reaction networks

    Davidescu, Florin Paul; Jørgensen, Sten Bay


    . The proposed analysis is performed in two phases. The first phase determines the structurally identifiable reaction rates based on reaction network stoichiometry. The second phase assesses the structural parameter identifiability of the specific kinetic rate expressions using a generating series expansion...... method based on Lie derivatives. The proposed systematic two phase methodology is illustrated on a mass action based model for an enzymatically catalyzed reaction pathway network where only a limited set of variables is measured. The methodology clearly pinpoints the structurally identifiable parameters...

  12. NRT Rotor Structural / Aeroelastic Analysis for the Preliminary Design Review

    Ennis, Brandon Lee [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Paquette, Joshua A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)


    This document describes the initial structural design for the National Rotor Testbed blade as presented during the preliminary design review at Sandia National Laboratories on October 28- 29, 2015. The document summarizes the structural and aeroelastic requirements placed on the NRT rotor for satisfactory deployment at the DOE/SNL SWiFT experimental facility to produce high-quality datasets for wind turbine model validation. The method and result of the NRT blade structural optimization is also presented within this report, along with analysis of its satisfaction of the design requirements.

  13. An Analysis of the Service Structure of China Post


    The environment of the postal industry in China has experienced tremendous changes in the past few years. The environmental changes have some effects on the elements that decide the service structure of China Post. In such context, the current service structure of China Post needs some improvements to adapt itself to the changing environment. Aiming at the optimization of the service structure of China Post, this paper reviews the law of its development and makes an analysis of its current characteristics and the existing problems.


    URDEA Mihaela


    Full Text Available This paper refers to linear static analysis of truss and beams structures modelled in SolidWorks. In SolidWorks truss and frame elements are available only for staticstudies. For illustration it was modelled and studied the behaviour of a support of pipe. The truss is a welded structure modelled with Weldment Structural Members and it is often found in industrial constructions. During this study, there have been investigated the following FEA steps: Pre-Processing, Processing and Post-Processing.The results of this study are images corresponding to Axial Stress and The Maximum Displacement in different situations.

  15. Computational analysis of RNA structures with chemical probing data.

    Ge, Ping; Zhang, Shaojie


    RNAs play various roles, not only as the genetic codes to synthesize proteins, but also as the direct participants of biological functions determined by their underlying high-order structures. Although many computational methods have been proposed for analyzing RNA structures, their accuracy and efficiency are limited, especially when applied to the large RNAs and the genome-wide data sets. Recently, advances in parallel sequencing and high-throughput chemical probing technologies have prompted the development of numerous new algorithms, which can incorporate the auxiliary structural information obtained from those experiments. Their potential has been revealed by the secondary structure prediction of ribosomal RNAs and the genome-wide ncRNA function annotation. In this review, the existing probing-directed computational methods for RNA secondary and tertiary structure analysis are discussed.

  16. Cost benefit analysis on different configurations of berthing structure

    Rajesh, G.; Saravanan, R.; Ravichandran, Vijaya; Parameswara Pandian, S.; Ramani Sujatha, Evangelin


    Port and harbors are essential for handling of the imports/exports of good transported through shipping. This paper discusses the different configuration of berthing structure, their design with respect to the site conditions and suitability. The analysis includes detailed load calculations conforming to the various codal provisions and design of the structure. The configuration of berthing structure considered are analyzed and designed using STAAD Pro for different combination of loads as per IS 4651. Bill of Quantities are prepared and final cost of construction is calculated. Factors affecting the construction and maintenance such as land availability, soil conditions, hydrodynamics of the site, dredging requirements, design ship size etc. are considered to finalize the configuration of the berthing structure. Result of the study shows that Diaphragm wall type of berthing structure is economic for Ennore port.

  17. Time-dependent reliability analysis and condition assessment of structures

    Ellingwood, B.R. [Johns Hopkins Univ., Baltimore, MD (United States)


    Structures generally play a passive role in assurance of safety in nuclear plant operation, but are important if the plant is to withstand the effect of extreme environmental or abnormal events. Relative to mechanical and electrical components, structural systems and components would be difficult and costly to replace. While the performance of steel or reinforced concrete structures in service generally has been very good, their strengths may deteriorate during an extended service life as a result of changes brought on by an aggressive environment, excessive loading, or accidental loading. Quantitative tools for condition assessment of aging structures can be developed using time-dependent structural reliability analysis methods. Such methods provide a framework for addressing the uncertainties attendant to aging in the decision process.

  18. RNAstructure: software for RNA secondary structure prediction and analysis

    Mathews David H


    Full Text Available Abstract Background To understand an RNA sequence's mechanism of action, the structure must be known. Furthermore, target RNA structure is an important consideration in the design of small interfering RNAs and antisense DNA oligonucleotides. RNA secondary structure prediction, using thermodynamics, can be used to develop hypotheses about the structure of an RNA sequence. Results RNAstructure is a software package for RNA secondary structure prediction and analysis. It uses thermodynamics and utilizes the most recent set of nearest neighbor parameters from the Turner group. It includes methods for secondary structure prediction (using several algorithms, prediction of base pair probabilities, bimolecular structure prediction, and prediction of a structure common to two sequences. This contribution describes new extensions to the package, including a library of C++ classes for incorporation into other programs, a user-friendly graphical user interface written in JAVA, and new Unix-style text interfaces. The original graphical user interface for Microsoft Windows is still maintained. Conclusion The extensions to RNAstructure serve to make RNA secondary structure prediction user-friendly. The package is available for download from the Mathews lab homepage at

  19. The lipid peroxidation product 4-hydroxy-trans-2-nonenal causes protein synthesis in cardiac myocytes via activated mTORC1-p70S6K-RPS6 signaling.

    Calamaras, Timothy D; Lee, Charlie; Lan, Fan; Ido, Yasuo; Siwik, Deborah A; Colucci, Wilson S


    Reactive oxygen species (ROS) are elevated in the heart in response to hemodynamic and metabolic stress and promote hypertrophic signaling. ROS also mediate the formation of lipid peroxidation-derived aldehydes that may promote myocardial hypertrophy. One lipid peroxidation by-product, 4-hydroxy-trans-2-nonenal (HNE), is a reactive aldehyde that covalently modifies proteins thereby altering their function. HNE adducts directly inhibit the activity of LKB1, a serine/threonine kinase involved in regulating cellular growth in part through its interaction with the AMP-activated protein kinase (AMPK), but whether this drives myocardial growth is unclear. We tested the hypothesis that HNE promotes myocardial protein synthesis and if this effect is associated with impaired LKB1-AMPK signaling. In adult rat ventricular cardiomyocytes, exposure to HNE (10 μM for 1h) caused HNE-LKB1 adduct formation and inhibited LKB1 activity. HNE inhibited the downstream kinase AMPK, increased hypertrophic mTOR-p70S6K-RPS6 signaling, and stimulated protein synthesis by 27.1 ± 3.5%. HNE also stimulated Erk1/2 signaling, which contributed to RPS6 activation but was not required for HNE-stimulated protein synthesis. HNE-stimulated RPS6 phosphorylation was completely blocked using the mTOR inhibitor rapamycin. To evaluate if LKB1 inhibition by itself could promote the hypertrophic signaling changes observed with HNE, LKB1 was depleted in adult rat ventricular myocytes using siRNA. LKB1 knockdown did not replicate the effect of HNE on hypertrophic signaling or affect HNE-stimulated RPS6 phosphorylation. Thus, in adult cardiac myocytes HNE stimulates protein synthesis by activation of mTORC1-p70S6K-RPS6 signaling most likely mediated by direct inhibition of AMPK. Because HNE in the myocardium is commonly increased by stimuli that cause pathologic hypertrophy, these findings suggest that therapies that prevent activation of mTORC1-p70S6K-RPS6 signaling may be of therapeutic value.


    Д. Х. ХАРЛАМПИДИ; Тарасова, В. А.


    Reported about a method for conducting a structural analysis of the thermodynamic refrigerating machine on the basis of separation exergy of destruction on the external and internal independent parts, as well as removable and its components are inevitable. The methodology has allowed complex take into account the influence on the efficiency of the refrigerating machine thermal-hydraulic losses and estimates the components of the exergy destruction in the basic elements. The analysis of charac...

  1. On discriminant analysis techniques and correlation structures in high dimensions

    Clemmensen, Line Katrine Harder

    This paper compares several recently proposed techniques for performing discriminant analysis in high dimensions, and illustrates that the various sparse methods dier in prediction abilities depending on their underlying assumptions about the correlation structures in the data. The techniques...... generally focus on two things: Obtaining sparsity (variable selection) and regularizing the estimate of the within-class covariance matrix. For high-dimensional data, this gives rise to increased interpretability and generalization ability over standard linear discriminant analysis. Here, we group...

  2. Structured Analysis and the Data Flow Diagram: Tools for Library Analysis.

    Carlson, David H.


    This article discusses tools developed to aid the systems analysis process (program evaluation and review technique, Gantt charts, organizational charts, decision tables, flowcharts, hierarchy plus input-process-output). Similarities and differences among techniques, library applications of analysis, structured systems analysis, and the data flow…

  3. Structured Analysis and the Data Flow Diagram: Tools for Library Analysis.

    Carlson, David H.


    This article discusses tools developed to aid the systems analysis process (program evaluation and review technique, Gantt charts, organizational charts, decision tables, flowcharts, hierarchy plus input-process-output). Similarities and differences among techniques, library applications of analysis, structured systems analysis, and the data flow…

  4. Interface between 40S exit channel protein uS7/Rps5 and eIF2α modulates start codon recognition in vivo

    Visweswaraiah, Jyothsna; Hinnebusch, Alan G


    The eukaryotic pre-initiation complex (PIC) bearing the eIF2·GTP·Met-tRNAiMet ternary complex (TC) scans the mRNA for an AUG codon in favorable context. AUG recognition evokes rearrangement of the PIC from an open, scanning to a closed, arrested conformation. Cryo-EM reconstructions of yeast PICs suggest remodeling of the interface between 40S protein Rps5/uS7 and eIF2α between open and closed states; however, its importance was unknown. uS7 substitutions disrupting eIF2α contacts favored in the open complex increase initiation at suboptimal sites, and uS7-S223D stabilizes TC binding to PICs reconstituted with a UUG start codon, indicating inappropriate rearrangement to the closed state. Conversely, uS7-D215 substitutions, perturbing uS7-eIF2α interaction in the closed state, confer the opposite phenotypes of hyperaccuracy and (for D215L) accelerated TC dissociation from reconstituted PICs. Thus, remodeling of the uS7/eIF2α interface appears to stabilize first the open, and then the closed state of the PIC to promote accurate AUG selection in vivo. DOI: PMID:28169832

  5. Performance based analysis of hidden beams in reinforced concrete structures

    Helou Samir H.


    Full Text Available Local and perhaps regional vernacular reinforced concrete building construction leans heavily against designing slabs with imbedded hidden beams for flooring systems in most structures including major edifices. The practice is distinctive in both framed and in shear wall structures. Hidden beams are favoured structural elements due to their many inherent features that characterize them; they save on floor height clearance; they also save on formwork, labour and material cost. Moreover, hidden beams form an acceptable aesthetic appearance that does not hinder efficient interior space partitioning. Such beams have the added advantage of clearing the way for horizontal electromechanical ductwork. However, seismic considerations, in all likelihood, are seldom seriously addressed. The mentioned structural system of shallow beams is adopted in ribbed slabs, waffle slabs and at times with solid slabs. Ribbed slabs and waffle slabs are more prone to hidden beam inclusion due to the added effective height of the concrete section. Due to the presence of a relatively high reinforcement ratio at the joints the sections at such location tend to become less ductile with unreliable contribution to spandrel force resistance. In the following study the structural influence of hidden beams within slabs is investigated. With the primary focus on a performance based analysis of such elements within a structure. This is investigated with due attention to shear wall contribution to the overall behaviour of such structures. Numerical results point in the direction that the function of hidden beams is not as adequate as desired. Therefore it is strongly believed that they are generally superfluous and maybe eliminated altogether. Conversely, shallow beams seem to render the overall seismic capacity of the structure unreliable. Since such an argument is rarely manifested within the linear analysis domain; a pushover analysis exercise is thus mandatory for behaviour

  6. A structural genomics analysis of histidine kinase sensor domains

    Cheung, Jonah


    Histidine kinase sensors are a part of a two-component system of protein signaling in prokaryotes and lower eukaryotes that relay an external environmental signal to an adaptive internal cellular response. Signal transduction occurs via phosphotransfer between a sensor protein and a response regulator which interact in tandem. The sensor is usually a transmembrane protein that contains a conserved cytoplasmic histidine kinase transmitter domain and a modular periplasmic sensor domain. The response regulator is cytoplasmic protein that contains a receiver domain that interacts with the histidine kinase, and an output domain that interacts with regulators of transcription or chemotaxis. My work focuses on the X-ray structure determination of a variety of bacterial sensor domains, based on a structural genomics analysis of the entire sensor domain family. Structures of the NarX, DcuS, LisK, and DctB sensor domains have been solved to atomic resolution, some in both ligand-bound and ligand-free states. Two distinct structural folds have been revealed---all-alpha helical and mixed alpha-beta. An analysis of the structures reveals a possible mechanism of transmembrane signaling in histidine kinase sensors as a sliding-piston motion between transmembrane helices. Although there is great diversity in ligand binding, there appears to be a small number of distinct sensor domain folds for which structural representatives of two have been solved. A final synthesis of the structural information with a comprehensive bio-informatics analysis of all histidine kinase sensor domain sequences allows fold prediction for over 400 sensor domains, in a step towards mapping the entire structural landscape of this protein family.

  7. Conceptual design and structural analysis of the CFETR cryostat

    Wang, Zhen, E-mail:; Yang, Qingxi; Xu, Hao


    Highlights: • The CFETR cryostat is a large vacuum container surrounding the tokamak basic machine. • Two conceptual design schemes of CFETR cryostat were proposed. • A series of structural analyses were performed for cryostat cylinder. • The design of base section is feasible for cryostat. - Abstract: CFETR (China Fusion Engineering Test Reactor) is a new tokamak device, one important component of which is cryostat and it is now under designing by China national integration design group. The CFETR cryostat is a large single-wall vacuum-tight container surrounding the tokamak basic machine, which consists of top dome-shape lid, two cylindrical sections with circumferential stiffening ribs and bottom flat head. It shall provide a vacuum environment (10{sup −4} Pa) for the operation of the superconducting coils and all the loads that derive from cryostat itself and inner components should be transferred to the floor of tokamak pit. In this paper, two schemes of cryostat were proposed and then the structural analyses including seismic response analysis, elastic stress analysis and buckling analysis were performed to validate the conceptual design of CFETR cryostat. Based on the analysis results, it can be inferred that the cryostat II has a higher stiffness and stability. The structure of cryostat I needs to be improved against buckling and it is more difficult to manufacture for cryostat II due to its complex curved surface compared with cryostat I. Finally, the structural analysis for base section was performed and the design of main support was proved to be feasible. The design of CFETR cryostat has not been finalized and structural optimization still need to be proceeded based on the analysis results.

  8. Analysis of a Model for the Morphological Structure of Renal Arterial Tree: Fractal Structure

    Aurora Espinoza-Valdez


    experimental data measurements of the rat kidneys. The fractal dimension depends on the probability of sprouting angiogenesis in the development of the arterial vascular tree of the kidney, that is, of the distribution of blood vessels in the morphology generated by the analytical model. The fractal dimension might determine whether a suitable renal vascular structure is capable of performing physiological functions under appropriate conditions. The analysis can describe the complex structures of the development vasculature in kidney.

  9. Nonlinear damage detection in composite structures using bispectral analysis

    Ciampa, Francesco; Pickering, Simon; Scarselli, Gennaro; Meo, Michele


    Literature offers a quantitative number of diagnostic methods that can continuously provide detailed information of the material defects and damages in aerospace and civil engineering applications. Indeed, low velocity impact damages can considerably degrade the integrity of structural components and, if not detected, they can result in catastrophic failure conditions. This paper presents a nonlinear Structural Health Monitoring (SHM) method, based on ultrasonic guided waves (GW), for the detection of the nonlinear signature in a damaged composite structure. The proposed technique, based on a bispectral analysis of ultrasonic input waveforms, allows for the evaluation of the nonlinear response due to the presence of cracks and delaminations. Indeed, such a methodology was used to characterize the nonlinear behaviour of the structure, by exploiting the frequency mixing of the original waveform acquired from a sparse array of sensors. The robustness of bispectral analysis was experimentally demonstrated on a damaged carbon fibre reinforce plastic (CFRP) composite panel, and the nonlinear source was retrieved with a high level of accuracy. Unlike other linear and nonlinear ultrasonic methods for damage detection, this methodology does not require any baseline with the undamaged structure for the evaluation of the nonlinear source, nor a priori knowledge of the mechanical properties of the specimen. Moreover, bispectral analysis can be considered as a nonlinear elastic wave spectroscopy (NEWS) technique for materials showing either classical or non-classical nonlinear behaviour.

  10. Template matching method for the analysis of interstellar cloud structure

    Juvela, M


    The structure of interstellar medium can be characterised at large scales in terms of its global statistics (e.g. power spectra) and at small scales by the properties of individual cores. Interest has been increasing in structures at intermediate scales, resulting in a number of methods being developed for the analysis of filamentary structures. We describe the application of the generic template-matching (TM) method to the analysis of maps. Our aim is to show that it provides a fast and still relatively robust way to identify elongated structures or other image features. We present the implementation of a TM algorithm for map analysis. The results are compared against rolling Hough transform (RHT), one of the methods previously used to identify filamentary structures. We illustrate the method by applying it to Herschel surface brightness data. The performance of the TM method is found to be comparable to that of RHT but TM appears to be more robust regarding the input parameters, for example, those related t...

  11. Finite Element Analysis of Wrinkled Membrane Structures for Sunshield Applications

    Johnston, John D.; Brodeur, Stephen J. (Technical Monitor)


    The deployable sunshield is an example of a gossamer structure envisioned for use on future space telescopes. The basic structure consists of multiple layers of pretensioned, thin-film membranes supported by deployable booms. The prediction and verification of sunshield dynamics has been identified as an area in need of technology development due to the difficulties inherent in predicting nonlinear structural behavior of the membranes and because of the challenges involved. in ground testing of the full-scale structure. This paper describes a finite element analysis of a subscale sunshield that has been subjected to ground testing in support of the Next Generation Space Telescope (NGST) program. The analysis utilizes a nonlinear material model that accounts for wrinkling of the membranes. Results are presented from a nonlinear static preloading analysis and subsequent dynamics analyses to illustrate baseline sunshield structural characteristics. Studies are then described which provide further insight into the effect of membrane. preload on sunshield dynamics and the performance of different membrane modeling techniques. Lastly, a comparison of analytical predictions and ground test results is presented.

  12. Structural Analysis of the Pantograph Type IVTM in PGSFR

    Kim, S. H.; Koo, G. H. [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)


    The pantograph type IVTM (In.Vessel Transfer Machine) in PGSFR handle fuel assemblies inside the reactor core, which has six kinds of movements and transfers the new and spent core assemblies between the fuel transfer port and the reactor core. In the IVTM, the main tube supports the pantograph arm and the slot in which the movement of the pantograph arm is possible. The gripper mechanism is mounted to the gripper guide structure and the vertical movement of the gripper is possible by using the screw driver shaft. In this study, we performed the primary stress analysis by considering the IVTM 3D configuration. Also, the thermal stress analysis was carried out and the mode characteristics of the structure by the natural frequency analysis were analyzed. The IVTM is supported by the rotating plug, which has the maximum arm length of 1.125 m. When the fuel assemblies are inserted and withdrawn from the reactor core, the refueling design load of 24.5 kN is considered at the gripper. The primary stress analysis is carried out by considering the above design load and a dead weight. Fig. 2 shows the stress intensity distribution due to the refueling design load and the dead weight. As shown in this figure, the maximum stress intensity is 40.6 MPa. Also, from the Fig. 3, we can see that the maximum stress occurs at the rotation pin joint of the pantograph arm link. The maximum dIsplacement is 4mm, which occurs at the lowest end of the pantograph arm. The structural analyses are carried out to evaluate the structural integrity of the IVTM. The primary stress analysis for the refueling design load, the thermal stress analysis and the natural frequency analysis are performed, and the stresses and dIsplacements are evaluated.

  13. MUSTANG-MR structural sieving server: applications in protein structural analysis and crystallography.

    Arun S Konagurthu

    Full Text Available BACKGROUND: A central tenet of structural biology is that related proteins of common function share structural similarity. This has key practical consequences for the derivation and analysis of protein structures, and is exploited by the process of "molecular sieving" whereby a common core is progressively distilled from a comparison of two or more protein structures. This paper reports a novel web server for "sieving" of protein structures, based on the multiple structural alignment program MUSTANG. METHODOLOGY/PRINCIPAL FINDINGS: "Sieved" models are generated from MUSTANG-generated multiple alignment and superpositions by iteratively filtering out noisy residue-residue correspondences, until the resultant correspondences in the models are optimally "superposable" under a threshold of RMSD. This residue-level sieving is also accompanied by iterative elimination of the poorly fitting structures from the input ensemble. Therefore, by varying the thresholds of RMSD and the cardinality of the ensemble, multiple sieved models are generated for a given multiple alignment and superposition from MUSTANG. To aid the identification of structurally conserved regions of functional importance in an ensemble of protein structures, Lesk-Hubbard graphs are generated, plotting the number of residue correspondences in a superposition as a function of its corresponding RMSD. The conserved "core" (or typically active site shows a linear trend, which becomes exponential as divergent parts of the structure are included into the superposition. CONCLUSIONS: The application addresses two fundamental problems in structural biology: first, the identification of common substructures among structurally related proteins--an important problem in characterization and prediction of function; second, generation of sieved models with demonstrated uses in protein crystallographic structure determination using the technique of Molecular Replacement.

  14. Ambient Response Analysis of the Heritage Court Tower Building Structure

    Brincker, Rune; Andersen, P.


    In this paper an ambient response analysis of the Heritage Court Building structure is presented. The work is a part of a blind test organised by Professor Carlos Ventura, University of British Columbia. The response data were analysed using two different techniques: a non-parametric based on Fre...

  15. Structural Damage Prediction and Analysis for Hypervelocity Impact: Consulting


    A portion of the contract NAS8-38856, 'Structural Damage Prediction and Analysis for Hypervelocity Impacts,' from NASA Marshall Space Flight Center (MSFC), included consulting which was to be documented in the final report. This attachment to the final report contains memos produced as part of that consulting.

  16. Stochastic Analysis of Offshore Steel Structures An Analytical Appraisal

    Karadeniz, Halil


    Stochastic Analysis of Offshore Steel Structures provides a clear and detailed guide to advanced analysis methods of fixed offshore steel structures using 3D beam finite elements under random wave and earthquake loadings. Advanced and up-to-date research results are coupled with modern analysis methods and essential theoretical information to consider optimal solutions to structural issues. As these methods require and use knowledge of different subject matters, a general introduction to the key areas is provided. This is followed by in-depth explanations supported by design examples, relevant calculations and supplementary material containing related computer programmers. By combining this theoretical and practical approach Stochastic Analysis of Offshore Steel Structures cover a range of key concepts in detail including: ·         The basic principles of standard 3D beam finite elements and special connections, ·         Wave loading - from hydrodynamics to the calculation of wave load...

  17. Understanding the structure of data when planning for analysis ...

    Erna Kinsey

    structure that can include classroom, grade level, school, school dis- trict, province and .... question arises as to what the unit of analysis should be, and how to deal with the ... The proper sample size for these variables is of course the number.

  18. Automatic analysis of trabecular bone structure from knee MRI

    Marques, Joselene; Granlund, Rabia; Lillholm, Martin


    We investigated the feasibility of quantifying osteoarthritis (OA) by analysis of the trabecular bone structure in low-field knee MRI. Generic texture features were extracted from the images and subsequently selected by sequential floating forward selection (SFFS), following a fully automatic, un...

  19. Analysis and Design of Biological Materials and Structures

    Öchsner, Andreas; Altenbach, Holm


    This collection provides researchers and scientists with advanced analyses and materials design techniques in Biomaterials and presents mechanical studies of biological structures. In 16 contributions well known experts present their research on Stress and Strain Analysis, Material Properties, Fluid and Gas mechanics and they show related problems.

  20. Numerical equilibrium analysis for structured consumer resource models

    de Roos, A.M.; Diekmann, O.; Getto, P.; Kirkilionis, M.A.


    In this paper, we present methods for a numerical equilibrium and stability analysis for models of a size structured population competing for an unstructured re- source. We concentrate on cases where two model parameters are free, and thus existence boundaries for equilibria and stability boundaries

  1. Stochastic Response and Reliability Analysis of Hysteretic Structures

    Mørk, Kim Jørgensen

    During the last 30 years response analysis of structures under random excitation has been studied in detail. These studies are motivated by the fact that most of natures excitations, such as earthquakes, wind and wave loads exhibit randomly fluctuating characters. For safety reasons this randomness...

  2. Adjoint sensitivity analysis of high frequency structures with Matlab

    Bakr, Mohamed; Demir, Veysel


    This book covers the theory of adjoint sensitivity analysis and uses the popular FDTD (finite-difference time-domain) method to show how wideband sensitivities can be efficiently estimated for different types of materials and structures. It includes a variety of MATLAB® examples to help readers absorb the content more easily.

  3. Multisurface interface model for analysis of masonry structures

    Lourenço, P.B.; Rots, J.G.


    The performance of an interface elastoplastic constitutive model for the analysis of unreinforced masonry structures is evaluated. Both masonry components are discretized aiming at a rational unit-joint model able to describe cracking, slip, and crushing of the material. The model is formulated in t

  4. Structural Analysis of Basalt Fiber Reinforced Plastic Wind Turbine Blade

    Mengal Ali Nawaz


    Full Text Available In this study, Basalt fiber reinforced plastic (BFRP wind turbine blade was analyzed and compared with Glass fiber reinforced plastic blade (GFRP. Finite element analysis (FEA of blade was carried out using ANSYS. Data for FEA was obtained by using rule of mixture. The shell element in ANSYS was used to simulate the wind turbine blade and to conduct its strength analysis. The structural analysis and comparison of blade deformations proved that BFRP wind turbine blade has better strength compared to GFRP wind turbine blade.

  5. Structural Health Monitoring Analysis for the Orbiter Wing Leading Edge

    Yap, Keng C.


    This viewgraph presentation reviews Structural Health Monitoring Analysis for the Orbiter Wing Leading Edge. The Wing Leading Edge Impact Detection System (WLE IDS) and the Impact Analysis Process are also described to monitor WLE debris threats. The contents include: 1) Risk Management via SHM; 2) Hardware Overview; 3) Instrumentation; 4) Sensor Configuration; 5) Debris Hazard Monitoring; 6) Ascent Response Summary; 7) Response Signal; 8) Distribution of Flight Indications; 9) Probabilistic Risk Analysis (PRA); 10) Model Correlation; 11) Impact Tests; 12) Wing Leading Edge Modeling; 13) Ascent Debris PRA Results; and 14) MM/OD PRA Results.

  6. Weighing the Costs and Benefits of Renewables Portfolio Standards:A Comparative Analysis of State-Level Policy Impact Projections

    Chen, Cliff; Wiser, Ryan; Bolinger, Mark


    State renewables portfolio standards (RPS) have emerged as one of the most important policy drivers of renewable energy capacity expansion in the U.S. Collectively, these policies now apply to roughly 40% of U.S. electricity load, and may have substantial impacts on electricity markets, ratepayers, and local economies. As RPS policies have been proposed or adopted in an increasing number of states, a growing number of studies have attempted to quantify the potential impacts of these policies, focusing primarily on projecting cost impacts, but sometimes also estimating macroeconomic and environmental effects. This report synthesizes and analyzes the results and methodologies of 28 distinct state or utility-level RPS cost impact analyses completed since 1998. Together, these studies model proposed or adopted RPS policies in 18 different states. We highlight the key findings of these studies on the costs and benefits of RPS policies, examine the sensitivity of projected costs to model assumptions, assess the attributes of different modeling approaches, and suggest possible areas of improvement for future RPS analysis.

  7. Earthquake Analysis of Structure by Base Isolation Technique in SAP

    T. Subramani


    Full Text Available This paper presents an overview of the present state of base isolation techniques with special emphasis and a brief on other techniques developed world over for mitigating earthquake forces on the structures. The dynamic analysis procedure for isolated structures is briefly explained. The provisions of FEMA 450 for base isolated structures are highlighted. The effects of base isolation on structures located on soft soils and near active faults are given in brief. Simple case study on natural base isolation using naturally available soils is presented. Also, the future areas of research are indicated. Earthquakes are one of nature IS greatest hazards; throughout historic time they have caused significant loss offline and severe damage to property, especially to man-made structures. On the other hand, earthquakes provide architects and engineers with a number of important design criteria foreign to the normal design process. From well established procedures reviewed by many researchers, seismic isolation may be used to provide an effective solution for a wide range of seismic design problems. The application of the base isolation techniques to protect structures against damage from earthquake attacks has been considered as one of the most effective approaches and has gained increasing acceptance during the last two decades. This is because base isolation limits the effects of the earthquake attack, a flexible base largely decoupling the structure from the ground motion, and the structural response accelerations are usually less than the ground acceleration. In general, the increase of additional viscous damping in the structure may reduce displacement and acceleration responses of the structure. This study also seeks to evaluate the effects of additional damping on the seismic response when compared with structures without additional damping for the different ground motions.

  8. Analysis and Flexible Structural Modeling for Oscillating Wing Utilizing Aeroelasticity

    Shao Ke; Wu Zhigang; Yang Chao


    Making use of modal characteristics of the natural vibration of flexible structure to design the oscillating wing aircraft is proposed.A series of equations concerning the oscillating wing of flexible structures are derived. The kinetic equation for aerodynamic force coupled with elastic movement is set up, and relevant formulae are derived. The unsteady aerodynamic one in that formulae is revised. The design principle, design process and range of application of such oscillating wing analytical method are elaborated. A flexible structural oscillating wing model is set up, and relevant time response analysis and frequency response analysis are conducted. The analytical results indicate that adopting the new-type driving way for the oscillating wing will not have flutter problems and will be able to produce propulsive force. Furthermore, it will consume much less power than the fixed wing for generating the same lift.

  9. Analysis of structural macroeconomic indicators based on harmony approach

    Knyshenko, Tetyana


    Full Text Available In the article the application of fractal theory and proportions of «gold section» is considered to the analysis of macroeconomic indicators, the criteria of optimum and efficiency of structure of economy are selected. An economy is represented as treelike fractal, every level of which is characterized by potential of profits (by the issue and necessity in charges. The types of structural subsystems in an economy are selected: permanent and temporal, the last arise up at violation of systems optimum. An author drew conclusion that on the certain types of economic activity structural subsystems can be effective, but they can not be named optimum, as there are deviations from the «gold section» at the certain type of the technological mode, in the issue, intermediate consumption and GVP. A conclusion about the type of the technological mode based on analysis of investment of spheres and types of economic activity in forming of macroeconomic indexes.

  10. Structural Simulations and Conservation Analysis -Historic Building Information Model (HBIM

    C. Dore


    Full Text Available In this paper the current findings to date of the Historic Building Information Model (HBIM of the Four Courts in Dublin are presented. The Historic Building Information Model (HBIM forms the basis for both structural and conservation analysis to measure the impact of war damage which still impacts on the building. The laser scan survey was carried out in the summer of 2014 of the internal and external structure. After registration and processing of the laser scan survey, the HBIM was created of the damaged section of the building and is presented as two separate workflows in this paper. The first is the model created from historic data, the second a procedural and segmented model developed from laser scan survey of the war damaged drum and dome. From both models structural damage and decay simulations will be developed for documentation and conservation analysis.

  11. Structure learning by pruning in independent component analysis

    Nielsen, Andreas Brinch; Hansen, Lars Kai


    We discuss pruning as a means of structure learning in independent component analysis (ICA). Learning the structure is attractive in both signal processing and in analysis of abstract data, where it can assist model interpretation, generalizability and reduce computation. We derive the relevant s...... based methods and Bayesian methods, for both small and large samples. The Bayesian information criterion (BIC) seems to outperform both AIC and test sets as tools for determining the optimal dimensionality....... saliency expressions and compare with magnitude based pruning and Bayesian sparsification. We show in simulations that pruning is able to identify underlying structures without prior knowledge on the dimensionality of the model. We find, that for ICA, magnitude based pruning is as efficient as saliency...


    Muhammad Asaad


    Full Text Available This study aims to analyze market structure and manufacturing industry performance on the economy of North Sumatra. The analysis uses two approaches, namely SCP analysis and econometric model. The data used is the Survey of Large Medium 2005-2009. The result shows that the structure of the industry is more dominant, including tight oligopoly, only small parts which belong to loose oligopoly and oligopoly markets. Some industries which have important roles for the economy are the palm oil industry, food and beverage industry, rubber industry and rubber products, and iron and steel basic industries, and basic non-ferrous metals.Keywords: market structure, industry performance, manufacturing industry, local economyJEL classification numbers: L10, L20, L60

  13. 中药复方药物血清对CDKN2A和RPS3a基因在人卵巢癌细胞株SKOV3中表达的影响%Impacts of Chinese Herbal Compound Drug Serum on the Expressions of CDKN2A and RPS3a in Human Ovarian Cancer Cell Lines(SKOV3)

    王熙月; 韩凤娟; 汤欣; 吴效科; 侯丽辉


    目的 研究CDKN2A(细胞周期依赖性激酶抑制基因)和RPS3a基因(核糖体蛋白质S3a)在中药复方理冲生髓饮诱导人卵巢癌细胞株人卵巢浆液性乳头状囊腺癌(SKOV3)细胞凋亡中的作用.方法 将20只Wistar大鼠随机分为理冲生髓饮组和生理盐水组,灌胃给药,采血制备含药血清.提取生理盐水组及理冲生髓饮组肿瘤细胞mRNA,制备DNA探针并分别用Cy3和Cy5两种荧光染料标记,与人细胞凋亡寡核苷酸微阵列芯片进行杂交,经扫描、分析,得出药物作用后表达有差异的基因.结果 共筛选出显著差异表达基因66个,其中20个基因表达水平上调,46个基因表达水平下调.结论 理冲生髓饮可能通过上调CDKN2A基因和下调RPS3a基因,达到诱导卵巢癌细胞凋亡的目的.诱导细胞周期停滞,阻碍肿瘤细胞蛋白质合成,影响细胞正常代谢等,可能是其诱导细胞凋亡的作用机制.%Objective To study the effects of CDKN2A( cyclin - dependent kinase inhibitor gene )and RPS3a(ribosomal protein S3a)on apoptosis in human ovarian serious cyctadenocarcinoma( SKOV3 )induced by Chinese herbal compound formula,lichong shengsui yin. Methods 20 Wistar rats were randomized into a lichong shengsui yin group and a physiological saline group. The medication was done with gastric infusion. Blood was collected to prepare medicine - contained serum. mRNA of cancer cells in physiological saline group and lichong shengsui yin group were extracted. DNA prohe pin was prepared and two fluorescent dyes, Cy3 and Cy5 were used for laheling separately. The hybridization was done with human apoptosis oligonucleotide microarray chip. Through scanning and analyzing,the genes with expression differences after medicinal action were obtained. Results 66 genes with significant differences were screened totally. Of them,the expressions of 20 genes were up - regulated and those of 46 genes were down - regulated. Conclusion Lichong shengsui yin works on

  14. On the design and structural analysis of jet engine fan blade structures

    Amoo, Leye M.


    Progress in the design and structural analysis of commercial jet engine fan blades is reviewed and presented. This article is motivated by the key role fan blades play in the performance of advanced gas turbine jet engines. The fundamentals of the associated physics are emphasized. Recent developments and advancements have led to an increase and improvement in fan blade structural durability, stability and reliability. This article is intended as a high level review of the fan blade environment and current state of structural design to aid further research in developing new and innovative fan blade technologies.

  15. Analysis of residue conformations in peptides in Cambridge structural database and protein-peptide structural complexes.

    Raghavender, Upadhyayula Surya


    A comprehensive statistical analysis of the geometric parameters of peptide chains in a reduced dataset of protein-peptide complexes in Protein Data Bank (PDB) is presented. The angular variables describing the backbone conformations of amino acid residues in peptide chains shed insights into the conformational preferences of peptide residues interacting with protein partners. Nonparametric statistical approaches are employed to evaluate the interrelationships and associations in structural variables. Grouping of residues based on their structure into chemical classes reveals characteristic trends in parameter relationships. A comparison of canonical amino acid residues in free peptide structures in Cambridge structural database (CSD) with identical residues in PDB complexes, suggests that the information can be integrated from both the structural repositories enabling efficient and accurate modeling of biologically active peptides. © 2016 John Wiley & Sons A/S.

  16. ACT Payload Shroud Structural Concept Analysis and Optimization

    Zalewski, Bart B.; Bednarcyk, Brett A.


    Aerospace structural applications demand a weight efficient design to perform in a cost effective manner. This is particularly true for launch vehicle structures, where weight is the dominant design driver. The design process typically requires many iterations to ensure that a satisfactory minimum weight has been obtained. Although metallic structures can be weight efficient, composite structures can provide additional weight savings due to their lower density and additional design flexibility. This work presents structural analysis and weight optimization of a composite payload shroud for NASA s Ares V heavy lift vehicle. Two concepts, which were previously determined to be efficient for such a structure are evaluated: a hat stiffened/corrugated panel and a fiber reinforced foam sandwich panel. A composite structural optimization code, HyperSizer, is used to optimize the panel geometry, composite material ply orientations, and sandwich core material. HyperSizer enables an efficient evaluation of thousands of potential designs versus multiple strength and stability-based failure criteria across multiple load cases. HyperSizer sizing process uses a global finite element model to obtain element forces, which are statistically processed to arrive at panel-level design-to loads. These loads are then used to analyze each candidate panel design. A near optimum design is selected as the one with the lowest weight that also provides all positive margins of safety. The stiffness of each newly sized panel or beam component is taken into account in the subsequent finite element analysis. Iteration of analysis/optimization is performed to ensure a converged design. Sizing results for the hat stiffened panel concept and the fiber reinforced foam sandwich concept are presented.

  17. Semantic and Structural Analysis of TV Diving Programs

    Fei Wang; Jin-Tao Li; Yong-Dong Zhang; Shou-Xun Lin


    Automatic content analysis of sports videos is a valuable and challenging task. Motivated by analogies between a class of sports videos and languages, the authors propose a novel approach for sports video analysis based on compiler principles. It integrates both semantic analysis and syntactic analysis to automatically create an index and a table of contents for a sports video. Each shot of the video sequence is first annotated and indexed with semantic labels through detection of events using domain knowledge. A grammar-based parser is then constructed to identify the tree structure of the video content based on the labels. Meanwhile, the grammar can be used to detect and recover errors during the analysis. As a case study, a sports video parsing system is presented in the particular domain of diving. Experimental results indicate the proposed approach is effective.

  18. Structured Analysis and Supervision Applied on Heavy Fuel Oil Tanks

    LAKHOUA Mohamed Najeh


    Full Text Available This paper introduces the need for structured analysis and real time (SA-RT method of controlcommand applications in a thermal power plant (TPP using a supervisory control and data acquisition system (SCADA. Then, the architecture of a SCADA system in a TPP is presented. A significant example of a control-command application is presented. It is about the heavy fuel oil tanks of a TPP. Then an application of a structured analysis method, generally used in industry, on the basis of the SA-RT formalism is presented. In fact, different modules are represented and described: Context Diagram, Data Flows Diagram, Control Flows Diagram, State Transition Diagram, Timing Specifications and Requirements Dictionary. Finally, this functional and operational analysis allows us to assist the different steps of the specification, the programming and the configuration of a new tabular in a SCADA system.

  19. Multigroup Moderation Test in Generalized Structured Component Analysis

    Angga Dwi Mulyanto


    Full Text Available Generalized Structured Component Analysis (GSCA is an alternative method in structural modeling using alternating least squares. GSCA can be used for the complex analysis including multigroup. GSCA can be run with a free software called GeSCA, but in GeSCA there is no multigroup moderation test to compare the effect between groups. In this research we propose to use the T test in PLS for testing moderation Multigroup on GSCA. T test only requires sample size, estimate path coefficient, and standard error of each group that are already available on the output of GeSCA and the formula is simple so the user does not need a long time for analysis.


    Ana-Maria GEAMÃNU


    Full Text Available Tax havens have long been under the attention of numerous Governments and International Organizations which triggered the concern of an uneven playing field in the taxation area. As a result numerous amendments have been made to both their commercial and tax legislations in order to be in line with the internationally agreed tax standards. The aim of this article is to conduct a SWOT analysis on the offshore corporate structures found in the Caribbean landscape. Based on a selection process of the most commonly recognized tax havens in the Caribbean region and an analysis of their offshore companies at the level of incorporation, administration, activities conducted and costs, a set of frequently met characteristics have been identified which stand at the basis of the SWOT analysis. The results stand to present a comprehensive four dimension framework of the offshore corporate structures in regards to their strengths, weaknesses, opportunities and threats.

  1. Estructuras de elevadores de carga. // Dumbwaiter structure stress analysis.

    G. Escobar Travieso


    Full Text Available Este trabajo tiene como objetivo analizar las tensiones y deformaciones que ocurren en las estructuras del marco de carro,plataforma y cabina de elevadores de carga mediante la aplicación del Método de los Elementos Finitos, específicamentedel montaplatos de 300 Kg. de capacidad, con vista a realizar el análisis de resistencia, racionalización del peso y material ylas posibles vías de solución.Palabras claves: Elevador de carga, estructuras, análisis de tensiones, elementos finitos.________________________________________________________________________________AbstractThis paper deals with the tension and deformation analysis in structures of lifter mark, platform and cabin offreight lifters by means of the Finite Elements Method, specifically the 300 kgs capacity dumbwaiter, in orderto carry out the resistance analysis, weight and material rationalization.Key words: Dumbwaiter, structures, stress analysis, finite elements.

  2. Moving shape analysis and control applications to fluid structure interactions

    Moubachir, Marwan


    Problems involving the evolution of two- and three-dimensional domains arise in many areas of science and engineering. Emphasizing an Eulerian approach, Moving Shape Analysis and Control: Applications to Fluid Structure Interactions presents valuable tools for the mathematical analysis of evolving domains. The book illustrates the efficiency of the tools presented through different examples connected to the analysis of noncylindrical partial differential equations (PDEs), such as Navier-Stokes equations for incompressible fluids in moving domains. The authors first provide all of the details of existence and uniqueness of the flow in both strong and weak cases. After establishing several important principles and methods, they devote several chapters to demonstrating Eulerian evolution and derivation tools for the control of systems involving fluids and solids. The book concludes with the boundary control of fluid-structure interaction systems, followed by helpful appendices that review some of the advanced m...


    Ana-Maria GEAMÃNU


    Full Text Available Tax havens have long been under the attention of numerous Governments and International Organizations which triggered the concern of an uneven playing field in the taxation area. As a result numerous amendments have been made to both their commercial and tax legislations in order to be in line with the internationally agreed tax standards. The aim of this article is to conduct a SWOT analysis on the offshore corporate structures found in the Caribbean landscape. Based on a selection process of the most commonly recognized tax havens in the Caribbean region and an analysis of their offshore companies at the level of incorporation, administration, activities conducted and costs, a set of frequently met characteristics have been identified which stand at the basis of the SWOT analysis. The results stand to present a comprehensive four dimension framework of the offshore corporate structures in regards to their strengths, weaknesses, opportunities and threats.

  4. Bayesian large-scale structure inference and cosmic web analysis

    Leclercq, Florent


    Surveys of the cosmic large-scale structure carry opportunities for building and testing cosmological theories about the origin and evolution of the Universe. This endeavor requires appropriate data assimilation tools, for establishing the contact between survey catalogs and models of structure formation. In this thesis, we present an innovative statistical approach for the ab initio simultaneous analysis of the formation history and morphology of the cosmic web: the BORG algorithm infers the primordial density fluctuations and produces physical reconstructions of the dark matter distribution that underlies observed galaxies, by assimilating the survey data into a cosmological structure formation model. The method, based on Bayesian probability theory, provides accurate means of uncertainty quantification. We demonstrate the application of BORG to the Sloan Digital Sky Survey data and describe the primordial and late-time large-scale structure in the observed volume. We show how the approach has led to the fi...

  5. Process Analysis in Container Shipping Network Structure Form Change

    Chen Chao


    Full Text Available Being aimed at the influence of ship-size and cargo-demand changes on container shipping networks, to reveal the evolution process of container shipping networks structure form, this paper respectively designed the operation models for two major container shipping networks structure forms: Multi-port-calling network and Hub-and-spoke network, to maximizing the investment efficiency. Based on the above models, a comprehensively integrated operation model of container shipping networks is built and the evolution process of container shipping networks structure form with changing of both ship-size and cargo demands is analyzed. Finally, through a case study, results show that the comprehensive integrated operation model is very effective in the analysis of evolution process of container shipping networks structure forms.

  6. Total systems design analysis of high performance structures

    Verderaime, V.


    Designer-control parameters were identified at interdiscipline interfaces to optimize structural systems performance and downstream development and operations with reliability and least life-cycle cost. Interface tasks and iterations are tracked through a matrix of performance disciplines integration versus manufacturing, verification, and operations interactions for a total system design analysis. Performance integration tasks include shapes, sizes, environments, and materials. Integrity integrating tasks are reliability and recurring structural costs. Significant interface designer control parameters were noted as shapes, dimensions, probability range factors, and cost. Structural failure concept is presented, and first-order reliability and deterministic methods, benefits, and limitations are discussed. A deterministic reliability technique combining benefits of both is proposed for static structures which is also timely and economically verifiable. Though launch vehicle environments were primarily considered, the system design process is applicable to any surface system using its own unique filed environments.

  7. Safety estimation of structural systems via interval analysis

    Wang Xiaojun; Wang Lei; Qiu Zhiping


    Considering that the uncertain information has serious influences on the safety of structural systems and is always limited,it is reasonable that the uncertainties are generally described as interval sets.Based on the non-probabilistic set-theoretic theory,which is applied to measuring the safety of structural components and further combined with the branch-and-bound method for the probabilistic reliability analysis of structural systems,the non-probabilistic branch-and-bound method for determining the dominant failure modes of an uncertain structural system is given.Meanwhile,a new system safety measuring index obtained by the non-probabilistic set-theoretic model is investigated.Moreover,the compatibility between the classical probabilistic model as well as the proposed interval-set model will be discussed to verify the physical meaning of the safety measure in this paper.Some numerical examples are utilized to illustrate the validity and feasibility of the developed method.

  8. Analysis of stability of community structure across multiple hierarchical levels

    Li, Hui-Jia


    The analysis of stability of community structure is an important problem for scientists from many fields. Here, we propose a new framework to reveal hidden properties of community structure by quantitatively analyzing the dynamics of Potts model. Specifically we model the Potts procedure of community structure detection by a Markov process, which has a clear mathematical explanation. Critical topological information regarding to multivariate spin configuration could also be inferred from the spectral significance of the Markov process. We test our framework on some example networks and find it doesn't have resolute limitation problem at all. Results have shown the model we proposed is able to uncover hierarchical structure in different scales effectively and efficiently.

  9. Structure-Based Phylogenetic Analysis of the Lipocalin Superfamily.

    Balasubramanian Lakshmi

    Full Text Available Lipocalins constitute a superfamily of extracellular proteins that are found in all three kingdoms of life. Although very divergent in their sequences and functions, they show remarkable similarity in 3-D structures. Lipocalins bind and transport small hydrophobic molecules. Earlier sequence-based phylogenetic studies of lipocalins highlighted that they have a long evolutionary history. However the molecular and structural basis of their functional diversity is not completely understood. The main objective of the present study is to understand functional diversity of the lipocalins using a structure-based phylogenetic approach. The present study with 39 protein domains from the lipocalin superfamily suggests that the clusters of lipocalins obtained by structure-based phylogeny correspond well with the functional diversity. The detailed analysis on each of the clusters and sub-clusters reveals that the 39 lipocalin domains cluster based on their mode of ligand binding though the clustering was performed on the basis of gross domain structure. The outliers in the phylogenetic tree are often from single member families. Also structure-based phylogenetic approach has provided pointers to assign putative function for the domains of unknown function in lipocalin family. The approach employed in the present study can be used in the future for the functional identification of new lipocalin proteins and may be extended to other protein families where members show poor sequence similarity but high structural similarity.

  10. Design and Analysis of Muon Beam Stop Support Structures

    Okafor, Udenna [Northern Illinois Univ., DeKalb, IL (United States)


    The primary objective of this thesis is to design and analyze support structures to be used in the installation, test and final positioning of the MBS throughout the life of the Mu2e experiment. There several requirements for the MBS imposed by both the scope of the experiment and, other components within the DS bore. The functions of the MBS are: 1. To limit the induced rates in the Tracker, the Calorimeter and the Cosmic Ray Veto due to backsplash-and-secondary interactions, and 2. To reduce radiation levels external to the Detector solenoid. The structures used in supporting the MBS will also adhere to requirements imposed by its functions. These requirements are critical to the support structures and affect design decisions. Other requirements critical to the design are imposed by the weight, positional tolerance and assembly procedure of the MBS, and also, the magnetic field and vacuum dose rate of the DS bore. A detailed breakdown of how each requirement affects the structural design can be found in chapter 2. Chapter 3 describes the design of each support structure and its attachment to the MBS while chapter 4 describes the results from structural analysis of the support structures. Chapter 5 describes evaluation for the design through testing and calculations while the conclusion in chapter 6 reports the current status at the time of this thesis submission with a plan for future work to be completed until final design and installation.

  11. Recent advances in computational structural reliability analysis methods

    Thacker, Ben H.; Wu, Y.-T.; Millwater, Harry R.; Torng, Tony Y.; Riha, David S.


    The goal of structural reliability analysis is to determine the probability that the structure will adequately perform its intended function when operating under the given environmental conditions. Thus, the notion of reliability admits the possibility of failure. Given the fact that many different modes of failure are usually possible, achievement of this goal is a formidable task, especially for large, complex structural systems. The traditional (deterministic) design methodology attempts to assure reliability by the application of safety factors and conservative assumptions. However, the safety factor approach lacks a quantitative basis in that the level of reliability is never known and usually results in overly conservative designs because of compounding conservatisms. Furthermore, problem parameters that control the reliability are not identified, nor their importance evaluated. A summary of recent advances in computational structural reliability assessment is presented. A significant level of activity in the research and development community was seen recently, much of which was directed towards the prediction of failure probabilities for single mode failures. The focus is to present some early results and demonstrations of advanced reliability methods applied to structural system problems. This includes structures that can fail as a result of multiple component failures (e.g., a redundant truss), or structural components that may fail due to multiple interacting failure modes (e.g., excessive deflection, resonate vibration, or creep rupture). From these results, some observations and recommendations are made with regard to future research needs.

  12. The model of local mode analysis for structural acoustics of box structures

    Ngai, King-Wah

    Structure-borne noise is a new noise pollution problem emerging from railway concrete box structures in Hong Kong. Its low frequency noise with intermittent effect can cause considerable nuisance to neighborhoods. The tonal noise peaks in this low frequency range should be one of the important factors in structure-borne noise analysis. In the acoustic field, the deterministic analysis of all the resonant modes of vibration is generally considered as not practical. Many acoustic experts use the statistical energy analysis as the main tool for the noise investigation whereas the application of the experimental modal analysis in the structural acoustic problem is comparatively rare. In the past, most studies mainly focused on the structure-borne noise measurement and analysis. The detail study of the cause of structure-borne noise is lack, especially for the rectangular concrete box structure. In this dissertation, an experimental and analytical approach is adopted to study a typical concrete box model. This thesis aims at confirming the importance of modal analysis in the structure-borne noise study and then at identifying the local vibration modes along the cross-section of box structure. These local modes are responsible for the structure-borne noise radiation. The findings of this study suggest that the web of viaduct cross-section is not as rigid as assumed in the conventional viaduct design and the web face is likely to be more flexible in the vertical displacement of the concrete viaduct. Two types of local vibration modes along the cross-section are identified: the centre mode and the web mode. At the top panel of the viaduct, the centre mode has movement in the middle but not at the edges. The web mode has movement at the edges with the middle fixed. The combined centre and web mode has been found to be important in the structural acoustics of the concrete box structure. In the actual concrete viaduct, the coincidence frequency is especially low (often around

  13. Probabilistic Finite Element Analysis & Design Optimization for Structural Designs

    Deivanayagam, Arumugam

    This study focuses on implementing probabilistic nature of material properties (Kevlar® 49) to the existing deterministic finite element analysis (FEA) of fabric based engine containment system through Monte Carlo simulations (MCS) and implementation of probabilistic analysis in engineering designs through Reliability Based Design Optimization (RBDO). First, the emphasis is on experimental data analysis focusing on probabilistic distribution models which characterize the randomness associated with the experimental data. The material properties of Kevlar® 49 are modeled using experimental data analysis and implemented along with an existing spiral modeling scheme (SMS) and user defined constitutive model (UMAT) for fabric based engine containment simulations in LS-DYNA. MCS of the model are performed to observe the failure pattern and exit velocities of the models. Then the solutions are compared with NASA experimental tests and deterministic results. MCS with probabilistic material data give a good prospective on results rather than a single deterministic simulation results. The next part of research is to implement the probabilistic material properties in engineering designs. The main aim of structural design is to obtain optimal solutions. In any case, in a deterministic optimization problem even though the structures are cost effective, it becomes highly unreliable if the uncertainty that may be associated with the system (material properties, loading etc.) is not represented or considered in the solution process. Reliable and optimal solution can be obtained by performing reliability optimization along with the deterministic optimization, which is RBDO. In RBDO problem formulation, in addition to structural performance constraints, reliability constraints are also considered. This part of research starts with introduction to reliability analysis such as first order reliability analysis, second order reliability analysis followed by simulation technique that

  14. Structural Optimization of Slender Robot Arm Based on Sensitivity Analysis

    Zhong Luo


    Full Text Available An effective structural optimization method based on a sensitivity analysis is proposed to optimize the variable section of a slender robot arm. The structure mechanism and the operating principle of a polishing robot are introduced firstly, and its stiffness model is established. Then, a design of sensitivity analysis method and a sequential linear programming (SLP strategy are developed. At the beginning of the optimization, the design sensitivity analysis method is applied to select the sensitive design variables which can make the optimized results more efficient and accurate. In addition, it can also be used to determine the scale of moving step which will improve the convergency during the optimization process. The design sensitivities are calculated using the finite difference method. The search for the final optimal structure is performed using the SLP method. Simulation results show that the proposed structure optimization method is effective in enhancing the stiffness of the robot arm regardless of the robot arm suffering either a constant force or variable forces.

  15. Generating function approach to reliability analysis of structural systems


    The generating function approach is an important tool for performance assessment in multi-state systems. Aiming at strength reliability analysis of structural systems, generating function approach is introduced and developed. Static reliability models of statically determinate, indeterminate systems and fatigue reliability models are built by constructing special generating functions, which are used to describe probability distributions of strength (resistance), stress (load) and fatigue life, by defining composite operators of generating functions and performance structure functions thereof. When composition operators are executed, computational costs can be reduced by a big margin by means of collecting like terms. The results of theoretical analysis and numerical simulation show that the generating function approach can be widely used for probability modeling of large complex systems with hierarchical structures due to the unified form, compact expression, computer program realizability and high universality. Because the new method considers twin loads giving rise to component failure dependency, it can provide a theoretical reference and act as a powerful tool for static, dynamic reliability analysis in civil engineering structures and mechanical equipment systems with multi-mode damage coupling.

  16. Structure of RIASEC Scores in China: A Structural Meta-Analysis

    Long, Lirong; Tracey, Terence J. G.


    A structural meta-analysis was conducted to evaluate the fit of four different representations of the relations among RIASEC types, Holland's (1985, 1997) circular order model, Gati's (1991) three-group partition model, Rounds and Tracey's (1996) alternative three-group partition model, and Liu and Rounds' (2003) modified octant model, on 29…

  17. Structural optimization of precision instruments through modal analysis


    In order to improve the dynamic stability of precision instruments during the design process,a compositive design method based on modal analysis of structure is proposed.With uniform boundary conditions and material characters,the results of Finite Element Analysis(FEA)vary with models.It should be checked whether the model is correctly simplified.Modal experiments can be used for such purpose.The method combines the high efficiency and agility of FEA with the reliability and accuracy of experiments,and avoids the drawbacks of FEA or experiments,such as uncertainty of FEA and high cost of experiments.Taking rotor-frame structure as an example,this method is applied as follows: First the modal characters of structure are analyzed with FEA,and then the natural frequencies of the structure are tested by experiments to check the reliability of FEA method,and finally the design scheme is optimized by modifying structure parameters with confirmed FEA.

  18. Numerical Analysis of Structural Progressive Collapse to Blast Loads

    HAO Hong; WU Chengqing; LI Zhongxian; ABDULLAH A K


    After the progressive collapse of Ronan Point apartment in UK in 1968,intensive research effort had been spent on developing guidelines for design of new or strengthening the existing structures to prevent progressive collapse.However,only very few building design codes provide some rather general guidance,no detailed design requirement is given.Progressive collapse of the Alfred P.Murrah Federal building in Oklahoma City and the World Trade Centre (WTC) sparked again tremendous research interest on progressive collapse of structures.Recently,US Department of Defence (DoD) and US General Service Administration (GSA) issued guidelines for structure progressive collapse analysis.These two guidelines are most commonly used,but their accuracy is not known.This paper presents numerical analysis of progressive collapse of an example frame structure to blast loads.The DoD and GSA procedures are also used to analyse the same example structure.Numerical results are compared and discussed.The accuracy and the applicability of the two design guidelines are evaluated.

  19. Network structure detection and analysis of Shanghai stock market

    Sen Wu


    Full Text Available Purpose: In order to investigate community structure of the component stocks of SSE (Shanghai Stock Exchange 180-index, a stock correlation network is built to find the intra-community and inter-community relationship. Design/methodology/approach: The stock correlation network is built taking the vertices as stocks and edges as correlation coefficients of logarithm returns of stock price. It is built as undirected weighted at first. GN algorithm is selected to detect community structure after transferring the network into un-weighted with different thresholds. Findings: The result of the network community structure analysis shows that the stock market has obvious industrial characteristics. Most of the stocks in the same industry or in the same supply chain are assigned to the same community. The correlation of the internal stock prices’ fluctuation is closer than in different communities. The result of community structure detection also reflects correlations among different industries. Originality/value: Based on the analysis of the community structure in Shanghai stock market, the result reflects some industrial characteristics, which has reference value to relationship among industries or sub-sectors of listed companies.

  20. An Overview of Path Analysis: Mediation Analysis Concept in Structural Equation Modeling

    Jenatabadi, Hashem Salarzadeh


    This paper provides a tutorial discussion on path analysis structure with concept of structural equation modelling (SEM). The paper delivers an introduction to path analysis technique and explain to how to deal with analyzing the data with this kind of statistical methodology especially with a mediator in the research model. The intended audience is statisticians, mathematicians, or methodologists who either know about SEM or simple basic statistics especially in regression and linear/nonline...

  1. [Structure analysis of disease-related proteins using vibrational spectroscopy].

    Hiramatsu, Hirotsugu


    Analyses of the structure and properties of identified pathogenic proteins are important for elucidating the molecular basis of diseases and in drug discovery research. Vibrational spectroscopy has advantages over other techniques in terms of sensitivity of detection of structural changes. Spectral analysis, however, is complicated because the spectrum involves a substantial amount of information. This article includes examples of structural analysis of disease-related proteins using vibrational spectroscopy in combination with additional techniques that facilitate data acquisition and analysis. Residue-specific conformation analysis of an amyloid fibril was conducted using IR absorption spectroscopy in combination with (13)C-isotope labeling, linear dichroism measurement, and analysis of amide I band features. We reveal a pH-dependent property of the interacting segment of an amyloidogenic protein, β2-microglobulin, which causes dialysis-related amyloidosis. We also reveal the molecular mechanisms underlying pH-dependent sugar-binding activity of human galectin-1, which is involved in cell adhesion, using spectroscopic techniques including UV resonance Raman spectroscopy. The decreased activity at acidic pH was attributed to a conformational change in the sugar-binding pocket caused by protonation of His52 (pKa 6.3) and the cation-π interaction between Trp68 and the protonated His44 (pKa 5.7). In addition, we show that the peak positions of the Raman bands of the C4=C5 stretching mode at approximately 1600 cm(-1) and the Nπ-C2-Nτ bending mode at approximately 1405 cm(-1) serve as markers of the His side-chain structure. The Raman signal was enhanced 12 fold using a vertical flow apparatus.

  2. Lexical and constructional organization of argument structure: a contrastive analysis

    Pedersen, Johan


    exclusive focus on lexicalization patterns. Contrastive analysis may provide insight into differing ways of organizing grammatical information. Construction grammar (CXG) suggests that clausal core information is organized by integrating at least two construction types: A) schematic constructions, B...... of constructional specificity at which the core information is organized. English (and presumably other Germanic languages to some extent) tends to organize principal clausal information in schematic argument structure constructions, lea­ving secondary information for lexical (verbal) specification. Spanish (and...... presumably other Romance languages to some extent) seems to organize principal clausal information lexically in verbal argument structure constructions, leaving secondary information for schematically organized specification....

  3. Structural Analysis and Seismic Design for Cold Neutron Laboratory Building

    Wu, Sangik; Kim, Y. K.; Kim, H. R


    This report describes all the major results of the dynamic structural analysis and seismic design for the Cold Neutron Laboratory Building which is classified in seismic class II. The results are summarized of the ground response spectrum as seismic input loads, mechanical properties of subsoil, the buoyancy stability due to ground water, the maximum displacement of the main frame under the seismic load and the member design. This report will be used as a basic design report to maintenance its structural integrity in future.


    Ioana Raluca Goldbach


    Full Text Available The comparative analysis of organizational structures highlights similarities and differences between different factors influencing a company in its way of operation with the internal and/or external environment. The paper analyzes the organizational structures of some top companies from England, France and Romania that influence/determine the cultural organizational values and the norms elaborated and implemented, which, in time, become moral values and norms that acquire a social meaning, which means that they have been accepted by all the members of the organization.

  5. Linearly Polarized IR Spectroscopy Theory and Applications for Structural Analysis

    Kolev, Tsonko


    A technique that is useful in the study of pharmaceutical products and biological molecules, polarization IR spectroscopy has undergone continuous development since it first emerged almost 100 years ago. Capturing the state of the science as it exists today, "Linearly Polarized IR Spectroscopy: Theory and Applications for Structural Analysis" demonstrates how the technique can be properly utilized to obtain important information about the structure and spectral properties of oriented compounds. The book starts with the theoretical basis of linear-dichroic infrared (IR-LD) spectroscop

  6. Modeling high temperature materials behavior for structural analysis

    Naumenko, Konstantin


    This monograph presents approaches to characterize inelastic behavior of materials and structures at high temperature. Starting from experimental observations, it discusses basic features of inelastic phenomena including creep, plasticity, relaxation, low cycle and thermal fatigue. The authors formulate constitutive equations to describe the inelastic response for the given states of stress and microstructure. They introduce evolution equations to capture hardening, recovery, softening, ageing and damage processes. Principles of continuum mechanics and thermodynamics are presented to provide a framework for the modeling materials behavior with the aim of structural analysis of high-temperature engineering components.

  7. Structural Modeling and Analysis of a Wave Energy Converter

    Zurkinden, Andrew Stephen; Lambertsen, Søren Heide; Damkilde, Lars


    A fatigue analysis is being carried out for a wave energy converter subjected to ocean wave loads. The device is a bottom fixed structure, located in a shallow water environment. Interest is focused on the local stress response of a structural detail and a subsequent calculation of its fatigue life...... by using the rainflow counting approach. The wave energy converter is characterized by its ability to enter in a storm protection mode which - whenever extreme conditions occur - will drastically reduce the exposure to wave loads. The predicted fatigue life is calculated for two different control cases...

  8. Analysis methods for structure reliability of piping components

    Schimpfke, T.; Grebner, H.; Sievers, J. [Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) mbH, Koeln (Germany)


    In the frame of the German reactor safety research program of the Federal Ministry of Economics and Labour (BMWA) GRS has started to develop an analysis code named PROST (PRObabilistic STructure analysis) for estimating the leak and break probabilities of piping systems in nuclear power plants. The long-term objective of this development is to provide failure probabilities of passive components for probabilistic safety analysis of nuclear power plants. Up to now the code can be used for calculating fatigue problems. The paper mentions the main capabilities and theoretical background of the present PROST development and presents some of the results of a benchmark analysis in the frame of the European project NURBIM (Nuclear Risk Based Inspection Methodologies for Passive Components). (orig.)

  9. China’s Structural Energy Conservation: Analysis and Proposals

    张雷; 黄园淅


    Given the increasing dependence of development on energy consumption, an understanding of the rules and trends of national energy consumption is required for boosting social energy conservation. By using two models -- the structural evolution energy consumption model and the structural evolution per-unit energy consumption model, this article attempts to make an international comparison of the industrial structure development versus energy consumption patterns in the United States, the United Kingdom, France, Germany, Japan, and India. The results of this analysis indicate that the dominance of secondary industry over the industrial structure evolution process noticeably accelerates national energy consumption at the initial stage of modernization. Subsequently, a slowdown in energy consumption as a result of industrial structure development yields effects as the diversification process speeds up. Under such influence, energy consumption per unit of GDP follows an inverted U-shaped curve from rise to decline. As one of the world’s major energy producers and consumers, China has long employed a one- sided sector development policy. Under the influence of this policy, national energy conservation endeavors have been inhibited by the rigidity in its industry structural evolution. Thus, in China, energy consumption per unit of GDP has remained at a high level.

  10. In situ structural analysis of the human nuclear pore complex.

    von Appen, Alexander; Kosinski, Jan; Sparks, Lenore; Ori, Alessandro; DiGuilio, Amanda L; Vollmer, Benjamin; Mackmull, Marie-Therese; Banterle, Niccolo; Parca, Luca; Kastritis, Panagiotis; Buczak, Katarzyna; Mosalaganti, Shyamal; Hagen, Wim; Andres-Pons, Amparo; Lemke, Edward A; Bork, Peer; Antonin, Wolfram; Glavy, Joseph S; Bui, Khanh Huy; Beck, Martin


    Nuclear pore complexes are fundamental components of all eukaryotic cells that mediate nucleocytoplasmic exchange. Determining their 110-megadalton structure imposes a formidable challenge and requires in situ structural biology approaches. Of approximately 30 nucleoporins (Nups), 15 are structured and form the Y and inner-ring complexes. These two major scaffolding modules assemble in multiple copies into an eight-fold rotationally symmetric structure that fuses the inner and outer nuclear membranes to form a central channel of ~60 nm in diameter. The scaffold is decorated with transport-channel Nups that often contain phenylalanine-repeat sequences and mediate the interaction with cargo complexes. Although the architectural arrangement of parts of the Y complex has been elucidated, it is unclear how exactly it oligomerizes in situ. Here we combine cryo-electron tomography with mass spectrometry, biochemical analysis, perturbation experiments and structural modelling to generate, to our knowledge, the most comprehensive architectural model of the human nuclear pore complex to date. Our data suggest previously unknown protein interfaces across Y complexes and to inner-ring complex members. We show that the transport-channel Nup358 (also known as Ranbp2) has a previously unanticipated role in Y-complex oligomerization. Our findings blur the established boundaries between scaffold and transport-channel Nups. We conclude that, similar to coated vesicles, several copies of the same structural building block--although compositionally identical--engage in different local sets of interactions and conformations.

  11. Structural analysis of the JT-60SA cryostat vessel body

    Botija, José, E-mail: [Association EURATOM – CIEMAT, Avda. Complutense 40, 28040 Madrid (Spain); Alonso, Javier; Fernández, Pilar; Medrano, Mercedes; Ramos, Francisco; Rincon, Esther; Soleto, Alfonso [Association EURATOM – CIEMAT, Avda. Complutense 40, 28040 Madrid (Spain); Davis, Sam; Di Pietro, Enrico; Tomarchio, Valerio [Fusion for Energy, JT-60SA European Home Team, 85748 Garching bei Munchen (Germany); Masaki, Kei; Sakasai, Akira; Shibama, Yusuke [JAEA – Japan Atomic Energy Agency, Naka Fusion Institute, Ibaraki 311-0193 (Japan)


    Highlights: ► Structural analysis to validate the JT-60SA cryostat vessel body design. ► Design code ASME 2007 “Boiler and Pressure Vessel Code. Section VIII”. ► First buckling mode: load multiplier of 10.644, higher than the minimum factor 4.7. ► Elastic and elastic–plastic stress analysis meets ASME against plastic collapse. ► Bolted fasteners have been analyzed showing small gaps closed by strong welding. -- Abstract: The JT-60SA cryostat is a stainless steel vacuum vessel (14 m diameter, 16 m height) which encloses the Tokamak providing the vacuum environment (10{sup −3} Pa) necessary to limit the transmission of thermal loads to the components at cryogenic temperature. It must withstand both external atmospheric pressure during normal operation and internal overpressure in case of an accident. The paper summarizes the structural analyses performed in order to validate the JT-60SA cryostat vessel body design. It comprises several analyses: a buckling analysis to demonstrate stability under the external pressure; an elastic and an elastic–plastic stress analysis according to ASME VIII rules, to evaluate resistance to plastic collapse including localized stress concentrations; and, finally, a detailed analysis with bolted fasteners in order to evaluate the behavior of the flanges, assuring the integrity of the vacuum sealing welds of the cryostat vessel body.

  12. Design-Oriented Analysis of Aircraft Fuselage Structures

    Giles, Gary L.


    A design-oriented analysis capability for aircraft fuselage structures that utilizes equivalent plate methodology is described. This new capability is implemented as an addition to the existing wing analysis procedure in the Equivalent Laminated Plate Solution (ELAPS) computer code. The wing and fuselage analyses are combined to model entire airframes. The paper focuses on the fuselage model definition, the associated analytical formulation and the approach used to couple the wing and fuselage analyses. The modeling approach used to minimize the amount of preparation of input data by the user and to facilitate the making of design changes is described. The fuselage analysis is based on ring and shell equations but the procedure is formulated to be analogous to that used for plates in order to take advantage of the existing code in ELAPS. Connector springs are used to couple the wing and fuselage models. Typical fuselage analysis results are presented for two analytical models. Results for a ring-stiffened cylinder model are compared with results from conventional finite-element analyses to assess the accuracy of this new analysis capability. The connection of plate and ring segments is demonstrated using a second model that is representative of the wing structure for a channel-wing aircraft configuration.

  13. 3D Images of Materials Structures Processing and Analysis

    Ohser, Joachim


    Taking and analyzing images of materials' microstructures is essential for quality control, choice and design of all kind of products. Today, the standard method still is to analyze 2D microscopy images. But, insight into the 3D geometry of the microstructure of materials and measuring its characteristics become more and more prerequisites in order to choose and design advanced materials according to desired product properties. This first book on processing and analysis of 3D images of materials structures describes how to develop and apply efficient and versatile tools for geometric analysis

  14. Ulysses Data Analysis: Magnetic Topology of Heliospheric Structures

    Crooker, Nancy


    In this final technical report on research funded by a NASA grant, a project overview is given by way of summaries on nine published papers. Research has included: 1) Using suprathermal electron data to study heliospheric magnetic structures; 2) Analysis of magnetic clouds, coronal mass ejections (CME), and the heliospheric current sheet (HCS); 3) Analysis of the corotating interaction region (CIR) which develop from interactions between solar wind streams of different velocities; 4) Use of Ulysses data in the interpretation of heliospheric events and phenomena.

  15. Advanced stress analysis methods applicable to turbine engine structures

    Pian, Theodore H. H.


    The following tasks on the study of advanced stress analysis methods applicable to turbine engine structures are described: (1) constructions of special elements which contain traction-free circular boundaries; (2) formulation of new version of mixed variational principles and new version of hybrid stress elements; (3) establishment of methods for suppression of kinematic deformation modes; (4) construction of semiLoof plate and shell elements by assumed stress hybrid method; and (5) elastic-plastic analysis by viscoplasticity theory using the mechanical subelement model.

  16. Stress analysis for wall structure in mobile hot cell design

    Bahrin, Muhammad Hannan, E-mail:; Rahman, Anwar Abdul, E-mail:; Hamzah, Mohd Arif, E-mail:; Mamat, Mohd Rizal; Azman, Azraf; Hasan, Hasni [Prototype and Plant Development Centre, Technical Services Division, Malaysian Nuclear Agency (Malaysia)


    Malaysian Nuclear Agency is developing a Mobile Hot Cell (MHC) in order to handle and manage Spent High Activity Radioactive Sources (SHARS) such as teletherapy heads and irradiators. At present, there are only two units of MHC in the world, in South Africa and China. Malaysian Mobile Hot cell is developed by Malaysian Nuclear Agency with the assistance of IAEA expert, based on the design of South Africa and China, but with improved features. Stress analysis has been performed on the design in order to fulfil the safety requirement in operation of MHC. This paper discusses the loading analysis effect from the sand to the MHC wall structure.

  17. Analysis of genetic structure and relationship among nine indigenous Chinese chicken populations by the Structure program

    H. F. Li; W. Han; Y. F. Zhu; J. T. Shu; X. Y. Zhang; K. W. Chen


    The multi-locus model-based clustering method Structure program was used to infer the genetic structure of nine indigenous Chinese chicken (Gallus gallus) populations based on 16 microsatellite markers. Twenty runs were carried out at each chosen value of predefined cluster numbers $(K)$ under admixture model. The Structure program properly inferred the presence of genetic structure with 0.999 probabilities. The genetic structure not only indicated that the nine kinds of chicken populations were defined actually by their locations, phenotypes or culture, but also reflected the underlying genetic variations. At $K = 2$, nine chicken populations were divided into two main clusters, one light-body type, including Chahua chicken (CHA), Tibet chicken (TIB), Xianju chicken (XIA), Gushi chicken (GUS) and Baier chicken (BAI); and the other heavy-body type, including Beijing You chicken (YOU), Xiaoshan chicken (XIA), Luyuan chicken (LUY) and Dagu chicken (DAG). GUS and DAG were divided into independent clusters respectively when equaled 4, 5, or 6. XIA and BIA chicken, XIA and LUY chicken, TIB and CHA chicken still clustered together when equaled 6, 7, and 8, respectively. These clustering results were consistent with the breeding directions of the nine chicken populations. The Structure program also identified migrants or admixed individuals. The admixed individuals were distributed in all the nine chicken populations, while migrants were only distributed in TIB, XIA and LUY populations. These results indicated that the clustering analysis using the Structure program might provide an accurate representation of the genetic relationship among the breeds.

  18. Research on modal analysis of structural acoustic radiation using structural vibration modes and acoustic radiation modes

    LI Sheng; ZHAO Deyou


    Modal analysis of structural acoustic radiation from a vibrating structure is discussed using structural vibration modes and acoustic radiation modes based on the quadratic form of acoustic power. The finite element method is employed for discretisizing the structure.The boundary element method and Rayleigh integral are used for modeling the acoustic fluid.It is shown that the power radiated by a single vibration mode is to increase the radiated power and the effect of modal interaction can lead to an increase or a decrease or no change in the radiated power, moreover, control of vibration modes is a good way to reduce both vibration and radiated sound as long as the influence of interaction of vibration modes on sound radiation is insignificant. Stiffeners may change mode shapes of a plate and thus change radiation efficiency of the plate's modes. The CHIEF method is adopted to obtain an acoustic radiation mode formulation without the nonuniqueness difficulty at critical frequencies for three-dimensional structures by using Moore-Penrose inverse. A pulsating cube is involved to verify the formulation. Good agreement is obtained between the numerical and analytical solutions. The shapes and radiation efficiencies of acoustic radiation modes of the cube are discussed. The structural acoustic control using structural vibration modes and acoustic radiation modes are compared and studied.

  19. ERBB2 influences the subcellular localization of the estrogen receptor in tamoxifen-resistant MCF-7 cells leading to the activation of AKT and RPS6KA2.

    Pancholi, Sunil; Lykkesfeldt, Anne E; Hilmi, Caroline; Banerjee, Susana; Leary, Alexandra; Drury, Suzanne; Johnston, Stephen; Dowsett, Mitch; Martin, Lesley-Ann


    Acquired resistance to endocrine therapies remains a major clinical obstacle in hormone-sensitive breast tumors. We used an MCF-7 breast tumor cell line (Tam(R)-1) resistant to tamoxifen to investigate this mechanism. We demonstrate that Tam(R)-1 express elevated levels of phosphorylated AKT and MAPK3/1-activated RPS6KA2 compared with the parental MCF-7 cell line (MCF-7). There was no change in the level of total ESR between the two cell lines; however, the Tam(R)-1 cells had increased phosphorylation of ESR1 ser(167). SiRNA blockade of AKT or MAPK3/1 had little effect on ESR1 ser(167) phosphorylation, but a combination of the two siRNAs abrogated this. Co-localization studies revealed an association between ERBB2 and ESR1 in the Tam(R)-1 but not MCF-7 cells. ESR1 was redistributed to extranuclear sites in Tam(R)-1 and was less transcriptionally competent compared with MCF-7 suggesting that nuclear ESR1 activity was suppressed in Tam(R)-1. Tamoxifen resistance in the Tam(R)-1 cells could be partially overcome by the ERBB2 inhibitor AG825 in combination with tamoxifen, and this was associated with re-localization of ESR1 to the nucleus. These data demonstrate that tamoxifen-resistant cells have the ability to switch between ERBB2 or ESR1 pathways promoting cell growth and that pharmacological inhibition of ERBB2 may be a therapeutic strategy for overcoming tamoxifen resistance.

  20. Analysis of the Structure Ratios of the Funding Sources

    Maria Daniela Bondoc


    Full Text Available The funding sources of the assets and liabilities in the balance sheet include equity capitals and the debts of the entity. The analysis of the structure rates of the funding sources allows for making assessments related to the funding policy, highlighting the financial autonomy and how resources are provided. Using the literature specializing in economic and financial analysis, this paper aims at presenting these rates that focus, on the one hand, to reflect the degree of financial dependence (the rate of financial stability, the rate of global financial autonomy, the rate of on-term financial autonomy and on the other hand the debt structure (the rate of short-term debts, the global indebtedness rate, the on-term indebtedness rate. Based on the financial statements of an entity in the Argeş County, I analysed these indicators, and I drew conclusions and made assessments related to the autonomy, indebtedness and financial stability of the studied entity.

  1. Structure Learning by Pruning in Independent Component Analysis

    Kjems, Andreas; Hansen, Lars Kai


    We discuss pruning as a means of structure learning in independent component analysis. Sparse models are attractive in both signal processing and in analysis of abstract data, they can assist model interpretation, generalizability and reduce computation. We derive the relevant saliency expression...... methods, for both small and large samples. The Bayesian information criterion (BIC) seems to outperform both AIC and test sets as tools for determining the optimal degree of sparsity....... and compare with magnitude based pruning and Bayesian sparsification. We show in simulations that pruning is able to identify underlying sparse structures without prior knowledge on the degree of sparsity. We find that for ICA magnitude based pruning is as efficient as saliency based methods and Bayesian...

  2. Structure and Performance Analysis of Regenerative Electromagnetic Shock Absorber

    Longxin Zhen


    Full Text Available This paper analyzed the structure and principle of a regenerative electromagnetic shock absorber in detail. The innovative shock absorber resembles linear generator in principle and can generate electric power through the relative reciprocating motion between coil assembly and permanent magnet assembly. At the same time, the damping can remove discomfort caused by road roughness. The regenerated electric power can be recovered through battery. Analysis of magnetic flux density of the permanent magnet array of the innovative shock absorber was performed using ANSYS software based on the structure parameters given in the paper,then the performance parameters of the shock absorber was determined . Analysis and calculation results prove the viability of this shock absorber.

  3. Development of Probabilistic Structural Analysis Integrated with Manufacturing Processes

    Pai, Shantaram S.; Nagpal, Vinod K.


    An effort has been initiated to integrate manufacturing process simulations with probabilistic structural analyses in order to capture the important impacts of manufacturing uncertainties on component stress levels and life. Two physics-based manufacturing process models (one for powdered metal forging and the other for annular deformation resistance welding) have been linked to the NESSUS structural analysis code. This paper describes the methodology developed to perform this integration including several examples. Although this effort is still underway, particularly for full integration of a probabilistic analysis, the progress to date has been encouraging and a software interface that implements the methodology has been developed. The purpose of this paper is to report this preliminary development.

  4. Investigation of structural responses of breakwaters for green water based on fluid-structure interaction analysis

    Chi-Seung Lee


    Full Text Available In the present study, the structural response of breakwaters installed on container carriers against green water impact loads was numerically investigated on the basis of the fluid-structure interaction analysis. A series of numerical studies is carried out to induce breakwater collapse under such conditions, whereby a widely accepted fluid-structure interaction analysis technique is adopted to realistically consider the phenomenon of green water impact loads. In addition, the structural behaviour of these breakwaters under green water impact loads is investigated simultaneously throughout the transient analysis. A verification study of the numerical results is performed using data from actual collapse incidents of breakwaters on container carriers. On the basis of the results of a series of numerical analyses, the pressure distribution of green water was accurately predicted with respect to wave mass and velocity. It is expected that the proposed analytical methodology and predicted pressure distribution could be used as a practical guideline for the design of breakwaters on container carriers.

  5. Seismic response analysis of a nuclear reactor structure considering nonlinear soil-structure interaction

    Bhaumik, Lopamudra, E-mail: [University of Illinois at Urbana-Champaign (United States); Raychowdhury, Prishati, E-mail: [Indian Institute of Technology Kanpur (India)


    Highlights: • Seismic response analysis of an internal shearwall of a reactor is done. • Incremental dynamic analysis is performed with 30 recorded ground motions. • Equivalent viscous damping increases up to twice when nonlinear SSI is considered. • Roof drift demand increases up to 25% upon consideration of foundation nonlinearity. • Base shear, base moment and ductility reduce up to 62%, 40%, and 35%, respectively. - Abstract: This study focuses on the seismic response analysis of an internal shearwall of a typical Indian reactor resting on a medium dense sandy silty soil, incorporating the nonlinear behavior of the soil-foundation interface. The modeling is done in an open-source finite element framework, OpenSees, where the soil-structure interaction (SSI) is modeled using a Beam-on-Nonlinear-Winkler-Foundation (BNWF) approach. Static pushover analysis and cyclic analysis are performed followed by an incremental dynamic analysis (IDA) with 30 recorded ground motions. For performing IDA, the spectral acceleration of each motion corresponding to the fundamental period, S{sub a}(T{sub 1})is incremented from 0.1 g to 1.0 g with an increment step of 0.1 g. It is observed from the cyclic analysis that the equivalent viscous damping of the system increases upto twice upon incorporation of inelastic SSI. The IDA results demonstrate that the average peak base shear, base moment and displacement ductility demand reduces as much as 62%, 40%, and 35%, respectively, whereas the roof drift demand increases up to 25% upon consideration of foundation nonlinearity for the highest intensity motion. These observations indicate the need of critical consideration of nonlinear soil-structure interaction as any deficient modeling of the same may lead to an inaccurate estimation of the seismic demands of the structure.

  6. Structural Analysis of Grounding Damages on MS DEXTRA

    Pedersen, Preben Terndrup; Simonsen, Bo Cerup; Zhang, Shengming

    Sub-task 1.2 of DEXTREMEL deals with development of models for external ship collision and grounding dynamics and for internal ship structure dynamics. In order to get a better overview of the work performed in this task it has been decided to write two reports on the work. One dealing...... with internal and external collision dynamics and the present report which deals with structural analysis of grounding events.The first part of the present report is devoted to an energy balance for raking damage situations.Then follows a numerical study of the forces associated with cutting and crushing...... of the bottom of MS DEXTRA and other RoRo vessels.Key words: ship grounding, inner mechanics, structural damage, absorbed energy, bottom damage distribution, and Minorsky method....

  7. Statistical models of video structure for content analysis and characterization.

    Vasconcelos, N; Lippman, A


    Content structure plays an important role in the understanding of video. In this paper, we argue that knowledge about structure can be used both as a means to improve the performance of content analysis and to extract features that convey semantic information about the content. We introduce statistical models for two important components of this structure, shot duration and activity, and demonstrate the usefulness of these models with two practical applications. First, we develop a Bayesian formulation for the shot segmentation problem that is shown to extend the standard thresholding model in an adaptive and intuitive way, leading to improved segmentation accuracy. Second, by applying the transformation into the shot duration/activity feature space to a database of movie clips, we also illustrate how the Bayesian model captures semantic properties of the content. We suggest ways in which these properties can be used as a basis for intuitive content-based access to movie libraries.

  8. Structure analysis of growing network based on partial differential equations

    Junbo JIA


    Full Text Available The topological structure is one of the most important contents in the complex network research. Therein the node degree and the degree distribution are the most basic characteristic quantities to describe topological structure. In order to calculate the degree distribution, first of all, the node degree is considered as a continuous variable. Then, according to the Markov Property of growing network, the cumulative distribution function's evolution equation with time can be obtained. Finally, the partial differential equation (PDE model can be established through distortion processing. Taking the growing network with preferential and random attachment mechanism as an example, the PDE model is obtained. The analytic expression of degree distribution is obtained when this model is solved. Besides, the degree function over time is the same as the characteristic line of PDE. At last, the model is simulated. This PDE method of changing the degree distribution calculation into problem of solving PDE makes the structure analysis more accurate.

  9. Performance analysis, quality function deployment and structured methods

    Maier, M. W.

    Quality function deployment, (QFD), an approach to synthesizing several elements of system modeling and design into a single unit, is presented. Behavioral, physical, and performance modeling are usually considered as separate aspects of system design without explicit linkages. Structured methodologies have developed linkages between behavioral and physical models before, but have not considered the integration of performance models. QFD integrates performance models with traditional structured models. In this method, performance requirements such as cost, weight, and detection range are partitioned into matrices. Partitioning is done by developing a performance model, preferably quantitative, for each requirement. The parameters of the model become the engineering objectives in a QFD analysis and the models are embedded in a spreadsheet version of the traditional QFD matrices. The performance model and its parameters are used to derive part of the functional model by recognizing that a given performance model implies some structure to the functionality of the system.

  10. Comparative Traffic Performance Analysis of Urban Transportation Network Structures

    Amini, Behnam; Mojarradi, Morteza; Derrible, Sybil


    The network structure of an urban transportation system has a significant impact on its traffic performance. This study uses network indicators along with several traffic performance measures including speed, trip length, travel time, and traffic volume, to compare a selection of seven transportation networks with a variety of structures and under different travel demand conditions. The selected network structures are: modified linear, branch, grid, 3-directional grid, 1-ring web, 2-ring web, and radial. For the analysis, a base origin-destination matrix is chosen, to which different growth factors are applied in order to simulate various travel demand conditions. Results show that overall the 2-ring web network offers the most efficient traffic performance, followed by the grid and the 1-ring networks. A policy application of this study is that the branch, 3-directional grid, and radial networks are mostly suited for small cities with uncongested traffic conditions. In contrast, the 2-ring web, grid, and 1-r...

  11. Nonlinear analysis of the forced response of structural elements

    Nayfeh, A. H.; Mook, D. T.; Sridhar, S.


    A general procedure is presented for the nonlinear analysis of the forced response of structural elements to harmonic excitations. Internal resonances (i.e., modal interactions) are taken into account. All excitations are considered, with special consideration given to resonant excitations. The general procedure is applied to clamped-hinged beams. The results reveal that exciting a higher mode may lead to a larger response in a lower interacting mode, contrary to the results of linear analyses.

  12. Analysis of time-domain scattering by periodic structures

    Gao, Yixian; Li, Peijun


    This paper is devoted to the mathematical analysis of a time-domain electromagnetic scattering by periodic structures which are known as diffraction gratings. The scattering problem is reduced equivalently into an initial-boundary value problem in a bounded domain by using an exact transparent boundary condition. The well-posedness and stability of the solution are established for the reduced problem. Moreover, a priori energy estimates are obtained with minimum regularity requirement for the data and explicit dependence on the time.

  13. Structural decomposition analysis on energy intensity changes at regional level

    Hua Liao; Ce Wang; Zhi-Shuang Zhu; Xiao-Wei Ma


    As China's energy intensity fluctuated in recent years, it is necessary to examine whether this fluctuations happened at a regional level. This paper conducts a decomposition model by using the structural decomposition analysis (SDA) method at a regional level. Then this model is employed to empirically analyze the changes of Beijing's energy intensity. The conclusions are as follows: during 2002-2010, except petroleum, the energy intensity decreased and the changes were mostly attributed to ...

  14. Thermal and structural analysis of a filter vessel ceramic tubesheet

    Mallett, R.H. [Mallett Technology, Inc., Research Triangle Park, NC (United States); Swindeman, R.W. [Oak Ridge National Lab., TN (United States); Zievers, J.F. [Industrial Filter & Pump Mfg. Co., Cicero, IL (United States)


    A ceramic tubesheet assembly for a hot gas filter vessel is analyzed using the finite element method to determine stresses under differential pressure loading. The stresses include local concentration effects. Selection of the stress measures for evaluation of structural integrity is discussed. Specification of stress limits based upon limited data is considered. Stress results from this ongoing design analysis technology project are shown for one design concept.

  15. Practical Application of Finite Element Analysis to Aircraft Structural Design


    analysis and mechanical properties, including the equivalent inclusion method, elastic constants and internal friction in composites, finite element...intensity factors are not available. The inclusion of fracture constraints in the automated design process is a logical extension of present structural...September 30-Dctober 3, 1975, Proceedings. Volume 1. (A78-19026 06-01) Turin, Libreria Editrice Universitaria Levrotto e Bella, 1975, p. 291-300. In

  16. Fractal analysis of the hierarchic structure of fossil coal surface

    Alekseev, A.D.; Vasilenko, T.A.; Kirillov, A.K. [National Academy of Sciences, Donetsk (Ukraine)


    The fractal analysis is described as method of studying images of surface of fossil coal, one of the natural sorbent, with the aim of determining its structural surface heterogeneity. The deformation effect as a reduction in the dimensions of heterogeneity boundaries is considered. It is shown that the theory of nonequilibrium dynamic systems permits to assess a formation level of heterogeneities involved into a sorbent composition by means of the Hurst factor.

  17. Practical Soil-Shallow Foundation Model for Nonlinear Structural Analysis

    Moussa Leblouba; Salah Al Toubat; Muhammad Ekhlasur Rahman; Omer Mugheida


    Soil-shallow foundation interaction models that are incorporated into most structural analysis programs generally lack accuracy and efficiency or neglect some aspects of foundation behavior. For instance, soil-shallow foundation systems have been observed to show both small and large loops under increasing amplitude load reversals. This paper presents a practical macroelement model for soil-shallow foundation system and its stability under simultaneous horizontal and vertical loads. The model...

  18. Thermal analysis of a hypersonic wing test structure

    Sandlin, Doral R.; Swanson, Neil J., Jr.


    The three-dimensional finite element modeling techniques developed for the thermal analysis of a hypersonic wing test structure (HWTS) are described. The computed results are compared to measured test data. In addition, the results of a NASA two-dimensional parameter finite difference local thermal model and the results of a contractor two-dimensional lumped parameter finite difference local thermal model will be presented.

  19. A note on structured means analysis for a single group

    Beauducel, Andre


    The calculation of common factor means in structured means analysis (SMM) is considered. The SMM equations imply that the unique factors are defined as having zero means. It was shown within the one factor solution that this definition implies larger absolute common factor loadings to co-occur with larger absolute expectations of the observed variables in the single group case. This result was illustrated by means of a small simulation study. It is argued that the proportionality of factor lo...

  20. Further finite element structural analysis of FAST Load Assembly

    Frosi, Paolo, E-mail: [Associazione Euratom ENEA sulla Fusione, C.R. Frascati, Via E. Fermi 45, 00044 Frascati, Rome (Italy); Crescenzi, F.; Cucchiaro, A.; Roccella, S. [Associazione Euratom ENEA sulla Fusione, C.R. Frascati, Via E. Fermi 45, 00044 Frascati, Rome (Italy)


    The FAST (Fusion Advanced Study Torus) machine is a compact high magnetic field tokamak, that will allow to study in an integrated way the main operational issues relating to plasma-wall interaction, plasma operation and burning plasma physics in conditions relevant for ITER and DEMO. The present work deals with the structural analysis of the machine Load Assembly for a proposed new plasma scenario (10 MA – 8.5 T), aimed to increase the operational limits of the machine. A previous paper has dealt with an integrated set of finite element models (regarding a former reference scenario: 6.5 MA – 7.5 T) of the load assembly, including the Toroidal and Poloidal Field Coils and the supporting structure. This set of models has regarded the evaluation of magnetic field values, the evaluation of the electromagnetic forces and the temperatures in all the current-carrying conductors: these analysis have been a preparatory step for the evaluation of the stresses of the main structural components. The previous models have been analyzed further on and improved in some details, including the computation of the out-of-plane electromagnetic forces coming from the interaction between the poloidal magnetic field and the current flowing in the toroidal magnets. After this updating, the structural analysis has been executed, where all forces and temperatures, coming from the formerly mentioned most demanding scenario (10 MA – 8.5 T) and acting on the tokamak's main components, have been considered. The two sets of analysis regarding the reference scenario and the extreme one have been executed and a useful comparison has been carried on. The analyses were carried out by using the FEM code Ansys rel. 13.