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Sample records for rosa hybrida cv

  1. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’

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    Kamnoon Kanchanapoom

    2010-01-01

    Full Text Available A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’ was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

  2. Effects of BAP and TIBA on Shoot Proliferation of Rosa hybrida L. cv. Full House in in vitro Culture

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    S. Hajian

    2016-02-01

    Full Text Available Micropropagation is a proper approach to rapid and large-scale propagation of rootstocks and rose cultivars for huge demand of flower market. Proliferation rate of shoot is decreased drastically following several subcultures. Growth regulators have remarkable effects on the key phase of proliferation in micropropagation of this popular crop. In this research the effects of BAP and antiauxin of TIBA on quality and quantity of developed shoots in Rosa hybrida cv. Full House were studied. BAP and TIBA were applied at three concentrations of 0, 2.2 and 8.8 µmol in proliferation phase of micropropagation. The experiment was conducted based on factorial and completely randomized design with four replications. After two months, the percentage of proliferated explants, survived main and lateral shoot number, length of the main and lateral shoots, number of green leaves on the shoots, the average number of shoots with chlorotic and necrotic leaves, the average axillary shoot base diameter, fresh weight of shoots and number of shoots with necrotic tip were recorded. Analysis of variance indicated that BAP was ineffective on the number of the main shoot green leaves and decreasing number of shoots with necrotic tip, but enhanced other traits. The concentration of 8.8 µmol of BAP had greater effect than 2.2 µmol of this growth regulator on mentioned traits. The higher concentration of TIBA resulted to more shoot with necrotic tip. This antiauxin had anegative impact on shoot fresh weight, but the other parameters were not significantly affected.

  3. Developmental patterns of emission of scent compounds and related gene expression in roses of the cultivar Rosa x hybrida cv. 'Yves Piaget'.

    Science.gov (United States)

    Chen, Xiaomin; Baldermann, Susanne; Cao, Shuyan; Lu, Yao; Liu, Caixia; Hirata, Hiroshi; Watanabe, Naoharu

    2015-02-01

    2-Phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) are characteristic scent compounds in specific roses such as Rosa x hybrida cv. 'Yves Piaget'. We analyzed the endogenous concentrations and emission of 2PE and DMT during the unfurling process in different floral organs, as well as changes in transcript levels of the two key genes, PAR and OOMT2. The emission of both 2PE and DMT increased during floral development to reach peaks at the fully unfurled stage. The relative transcripts of PAR and OOMT2 also increased during floral development. Whereas the maximum for OOMT2 was found at the fully unfurled stage (stage 4), similar expression levels of PAR were detected at stage 4 and the senescence stage (stage 6). The results demonstrate a positive correlation between the expression levels of PAR and OOMT2 and the emission of 2PE and DMT. In addition, endogenous volatiles and relative transcripts showed tissue- and development-specific patterns. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  4. Response of selected antioxidants and pigments in tissues of Rosa hybrida and Fuchsia hybrida to supplemental UV-A exposure

    NARCIS (Netherlands)

    Helsper, J.P.F.G.; Vos, de C.H.; Maas, F.M.; Jonker, H.H.; Broeck, van den H.C.; Jordi, W.; Pot, C.S.; Keizer, L.C.P.; Schapendonk, A.H.C.M.

    2003-01-01

    The effect of supplemental UV-A (320-400 nm) radiation on tissue absorption at 355 nm, levels of various antioxidants (ascorbate, glutathione, carotenoids and flavonoids) and of antioxidant scavenging capacity were investigated with leaves and petals of Rosa hybrida, cv. Honesty and with leaves,

  5. Transformation of miniature potted rose (Rosa hybrida cv. Linda) with P( SAG12 )-ipt gene delays leaf senescence and enhances resistance to exogenous ethylene.

    Science.gov (United States)

    Zakizadeh, Hedayat; Lütken, Henrik; Sriskandarajah, Sridevy; Serek, Margrethe; Müller, Renate

    2013-02-01

    KEY MESSAGE : The P ( SAG12 ) -ipt gene was transferred to miniature rose, as the first woody species, resulting in increased ethylene resistance due to specific up-regulation of the ipt gene under senescence promoting conditions. Transgenic plants of Rosa hybrida 'Linda' were obtained via transformation with Agrobacterium tumefaciens strain harboring the binary vector pSG529(+) containing the P( SAG12 )-ipt construct. A. tumefaciens strains AGL1, GV3850 and LBA4404 (containing P(35S)-INTGUS gene) were used for transformation of embryogenic callus, but transgenic shoots were obtained only when AGL1 was applied. The highest transformation frequency was 10 % and it was achieved when half MS medium was used for the dilution of overnight culture of Agrobacterium. Southern blot confirmed integration of 1-6 copies of the nptII gene into the rose genome in the tested lines. Four transgenic lines were obtained which were morphologically true-to-type and indistinguishable from Wt shoots while they were in in vitro cultures. Adventitious root induction was more difficult in transgenic shoots compared to the Wt shoots, however, one of the transgenic lines (line 6) was rooted and subsequently analyzed phenotypically. The ipt expression levels were determined in this line after exposure to exogenous ethylene (3.5 μl l(-1)) and/or darkness. Darkness resulted in twofold up-regulation of ipt expression, whereas darkness combined with ethylene caused eightfold up-regulation in line 6 compared to Wt plants. The transgenic line had significantly higher content of chlorophyll at the end of the treatment period compared to Wt plants.

  6. Transformation of miniature potted rose (Rosa hybrida cv. Linda) with PSAG12-ipt gene delays leaf senescence and enhances resistance to exogenous ethylene

    DEFF Research Database (Denmark)

    Zakizadeh, Hedayatollah; Lütken, Henrik Vlk; Sriskandarajah, Sridevy

    2013-01-01

    Transgenic plants of Rosa hybrida ‘Linda’ were obtained via transformation with Agrobacterium tumefaciens strain harboring the binary vector pSG529(+) containing the PSAG12-ipt construct. A. tumefaciens strains AGL1, GV3850 and LBA4404 (containing P35S-INTGUS gene) were used for transformation...... of embryogenic callus, but transgenic shoots were obtained only when AGL1 was applied. The highest transformation frequency was 10 % and it was achieved when half MS medium was used for the dilution of overnight culture of Agrobacterium. Southern blot confirmed integration of 1–6 copies of the nptII gene...

  7. Responses of air humidity and light quality on growth and stomata function of greenhouse grown Rosa*hybrida

    OpenAIRE

    Poudel, Madhu Sudhan

    2013-01-01

    Single node stem segment with one mature leaf of Rosa x hybrida, cv. Toril were grown in chambers. Plants were exposed to 100 μmol m-2 s-1 for 20 h day-1 followed by a 4 h dark period and supplementary light was provided by LED (20% blue light and 80% red light), HPS and HPS+UV-B Lamp. Each light source was provided by moderate (60%) and high (90) RH in different chambers. The UV-B light was provided 1 hour twice a day, in the middle of the dark period and in the middle of ligh...

  8. Introduction of ROL genes in Rosa hybrida L. for improved rootstock performance

    NARCIS (Netherlands)

    Salm, van der T.P.M.

    1996-01-01


    In contrast to many other cut flowers, which are harvested completely at the time of flowering, rose plants ( Rosahybrida L.) continuously produce flowering shoots, which are harvested on a regular basis. For the release of axillary buds and

  9. Root temperature effects on growth and bud break of Rosa hybrida in relation to cytokinin concentrations in xylem sap.

    NARCIS (Netherlands)

    Dieleman, J.A.; Verstappen, F.W.A.; Kuiper, D.

    1998-01-01

    The effects of three divergent root temperatures (11°C, 20°C and 26°C) on growth and bud break of Rosa hybrida were studied. Root morphology was changed considerably with root temperature. Roots at 11°C were white, succulent, short and sparsely branched, whereas at 26°C roots were long, brown, thin

  10. Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu

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    N. G. Ribeiro-Júnior

    Full Text Available Abstract The quality of forage production is a prerequisite to raising livestock. Therefore, income losses in this activity, primarily cattle raising, can result in the impossibility of economic activity. Through the qualitative and quantitative anatomical study of Urochloa hybrida cv. Mulato II and U. brizantha cv. Marandu, we searched for descriptions and compared changes in the individual vegetative body from populations with death syndrome pastures (DPS. Specimens were collected at different physiological stages from farms in northern Mato Grosso. After collection, the individuals were fixed in FAA50 and stored in 70% alcohol. Histological slides were prepared from the middle third of the sections of roots, rhizomes, and leaves, and the proportions and characteristics of tissues were evaluated in healthy, intermediate, and advanced stages of DPS. Changes were compared between cultivars. With the advancement of the syndrome, the following changes were observed: a more marked decrease in the length of roots in U. hybrida; disorganization of the cortical region of the roots and rhizome cultivars; fungal hyphae in roots and aerenchyma formation in U. hybrida; a decrease in sclerenchyma fiber proportions in roots and leaves; sclerification of the epidermis of U. brizantha rhizomes; and an increase in pericyclic fibers in U. hybrida. Furthermore, there was a decrease in the volume of epidermal cells of the abaxial face of the leaves of both cultivars, with a greater reduction in U. hybrida; a gradual decrease in thickness in the midrib of leaves similar to leaf mesophyll; conduction system obstructions; partial or total cell lysis in roots and rhizomes affected by the syndrome. Obstructions in sieve tube element and companion cells, and sometimes obstruction in xylem vessel elements. The evolution of DPS in cultivars was similar, but there were variations, arising probably from the physiological response to stress, such as aerenchyma formation in

  11. Effect of nano-silver and boric acid on extending the vase life of cut rose (Rosa hybrida L.).

    Science.gov (United States)

    Hashemabadi, Davood; Liavali, Mahbanoo Hoseinzadeh; Kaviani, Behzad; Mousavi, Meysam; Keyghobadi, Saghi; Zahiri, Samaneh

    2014-09-01

    Silver nano-particles (2-5 nm diam.), as antimicrobial agent and boric acid, as ethylene production inhibitor are used for enhancing the quality and vase life of cut flowers. In the present study the effects of a preservative solution containing nano-silver and boric acid on some traits of cut rose (Rosa hybrida L. cv. Yellow Island) including vase life, ethylene production, dry weight percentage, chlorophyll content, flower opening index, beta-carotene of petals and the number of basal stem end bacteria were investigated. The results showed that the effect of nano-silver and boric acid as either solitary or in combination with each other were significant (p rose treated with 100 mg l(-1) boric acid along with 5 mg l(-1) nano-silver. The lowest number of bacteria in the end of stem was calculated in cut flowers treated with the highest concentrations of boric acid (300 mg l(-1)) and nano-silver (20 mg l(-1)).

  12. In vitro mutagenesis for development of novel variants in Rose (Rosa X Hybrida)

    International Nuclear Information System (INIS)

    Namita, B.; Raju, D.V.S.; Prasad, K.V.; Singh, Kanwar P.

    2014-01-01

    Rose (Rosa x hybrida) is the top ranking cut flower in international market due to its attractive flowers with longer stem and fragrance. Modern roses are the result of hybridization, selection and spontaneous mutations. In floriculture industry, the demand of the novel varieties of rose is increasing day by day due to change in trends and taste. Mutation breeding is an already established method for improvement of flower crops. It offers several advantages over conventional methods for the improvement of one or more traits within a short span of time. Induced mutation is one of the potent methods to induce variability in existing rose genotypes. Mutation breeding combined with tissue culture i.e. in vitro mutagenesis has proven more effective in rose due to controlled environment that provides ideal conditions for survival of mutated tissues or cells. Keeping these aspects in view, the present investigation was carried out to induce variability in rose cv. Grand Gala under in vitro conditions using gamma (γ) rays. Single node cuttings were excised from the field grown plants and were irradiated with different doses of γ rays (0,10, 20, 30, 40, 50, 60, 70 and 80 Gy) using a 60 Co source. The γ-irradiated explants were then pretreated, surface sterilized and cultured aseptically on Murashige and Skoog basal medium supplemented with 2.5 mg 5-benzylaminopurine (BAP) plus 5.0 mg kinetin plus 0.1 mg a-naphthaleneacetic acid (NAA) plus 0.5 mg gibberellic acid (GA 3 ) plus 40 mg adenine sulphate plus 0.8% (w/v) agar-agar to induce sprouting and shoot proliferation. Explants treated at higher dose of γ-rays (70 and 80 Gy) showed deleterious effects of ionising radiation. The 30 Gy treatment was found to be the LD 50 dose. It was observed that few explants treated with γ-rays sprouted, showed slow growth and failed to survive after the first sub culture. The explants irradiated with 50 Gy γ-rays exhibited minimum explant survival, bud sprouting, number of shoots

  13. Evaluation of growth and flowering potential of rosa hybrida cultivars under Faisalabad climatic conditions

    International Nuclear Information System (INIS)

    Nadeem, M.; Khan, M.A.; Riaz, A.

    2011-01-01

    Exotic cultivars of hybrid roses respond uncertainly to new habitat. It is necessary to explore the potential of the introduced cultivars to judge the suitability in a new habitat. In the present study, nine Rosa hybrida cultivars including Autumn Sunset, Ice Berg, Paradise, Angel Face, Louise Odier, Casino, Grand Margina, Handel and Gruss-an-Teplitz were evaluated for growth and yield attributed under the climatic conditions of Faisalabad. Results indicated that there was decreasing trend in the growth and flowering of the bushes as the temperature increased above 32 degree C and humidity decreased to 29 %. Number of flowers per bush and diameter of flower decreased as the temperature increased and humidity decreased in contrast to increment in height of the plant and num ber of primary branches per plant in succeeding months. Interaction between yield traits and months was also significant. Overall, significant variations were observed in each cultivar for length and number of petals per flower, number of prickles, fragrance, flower persistence life and color, bush shape and overall performance with respect to climatic conditions of Faisalabad. It is concluded that the cultivars 'Autumn Sunset' and Gruss-an-Teplitz performed better in climatic conditions of Faisalabad. (author)

  14. A simulation model of Rosa hybrida growth response to constant irradiance and day and night temperatures

    International Nuclear Information System (INIS)

    Hopper, D.A.; Hammer, P.A.; Wilson, J.R.

    1994-01-01

    This paper details the development and verification of ROSESIM, a computer simulation model of the growth of ‘Royalty’ roses (Rosa hybrida L.) based on experimentally observed growth responses from pinch until flowering under 15 combinations of constant photosynthetic photon flux (PPF), day temperature (DT), and night temperature (NT). Selected according to a rotatable central composite design, these treatment combinations represent commercial greenhouse conditions during the winter and spring in the midwestern United States; each selected condition was maintained in an environmental growth chamber having 12-hour photoperiods. ROSESIM incorporates regression models of four flower development characteristics (days from pinch to visible bud, first color, sepal reflex, and flowering) that are full quadratic polynomials in PPF, DT, and NT. ROSESIM also incorporates mathematical models of nine plant growth characteristics (stem length and the following fresh and dry weights: stem, leaf, flower, and total) based on data recorded every 10 days and at flowering. At each design point, a cubic regression in time (days from pinch) estimated the plant growth characteristics on intermediate days; then difference equations were developed to predict the resulting daily growth increments as third-degree polynomial functions of days from pinch, PPF, DT, and NT. ROSESIM was verified by plotting against time each simulated plant growth characteristic and the associated experimental observations for the eight factorial design points defining the region of interest. Moreover, one-way analysis of variance procedures were applied to the differences between ROSESIM predictions and the corresponding observed means for all 15 treatment combinations. At 20 days from pinch, significant differences (P < 0.05) were observed for all nine plant growth characteristics. At 30 and 40 days from pinch, only flower fresh and dry weights yielded significant differences; at flowering, none of the 13

  15. Regression models describing Rosa hybrida response to day/night temperature and photosynthetic photon flux

    International Nuclear Information System (INIS)

    Hopper, D.A.; Hammer, P.A.

    1991-01-01

    A central composite rotatable design was used to estimate quadratic equations describing the relationship of irradiance, as measured by photosynthetic photon flux (PPF), and day (DT) and night (NT) temperatures to the growth and development of Rosa hybrida L. in controlled environments. Plants were subjected to 15 treatment combinations of the PPF, DT, and NT according to the coding of the design matrix. Day and night length were each 12 hours. Environmental factor ranges were chosen to include conditions representative of winter and spring commercial greenhouse production environments in the midwestern United States. After an initial hard pinch, 11 plant growth characteristics were measured every 10 days and at flowering. Four plant characteristics were recorded to describe flower bud development. Response surface equations were displayed as three-dimensional plots, with DT and NT as the base axes and the plant character on the z-axis while PPF was held constant. Response surfaces illustrated the plant response to interactions of DT and NT, while comparisons between plots at different PPF showed the overall effect of PPF. Canonical analysis of all regression models revealed the stationary point and general shape of the response surface. All stationary points of the significant models were located outside the original design space, and all but one surface was a saddle shape. Both the plots and analysis showed greater stem diameter, as well as higher fresh and dry weights of stems, leaves, and flower buds to occur at flowering under combinations of low DT (less than or equal to 17C) and low NT (less than or equal to 14C). However, low DT and NT delayed both visible bud formation and development to flowering. Increased PPF increased overall flower stem quality by increasing stem diameter and the fresh and dry weights of all plant parts at flowering, as well as decreased time until visible bud formation and flowering. These results summarize measured development at

  16. A simulation model of Rosa hybrida growth response to constant irradiance and day and night temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Hopper, D. A. [Colorado State University, Fort Collin, CO. (United States); Hammer, P. A.; Wilson, J. R.

    1994-09-15

    This paper details the development and verification of ROSESIM, a computer simulation model of the growth of ‘Royalty’ roses (Rosa hybrida L.) based on experimentally observed growth responses from pinch until flowering under 15 combinations of constant photosynthetic photon flux (PPF), day temperature (DT), and night temperature (NT). Selected according to a rotatable central composite design, these treatment combinations represent commercial greenhouse conditions during the winter and spring in the midwestern United States; each selected condition was maintained in an environmental growth chamber having 12-hour photoperiods. ROSESIM incorporates regression models of four flower development characteristics (days from pinch to visible bud, first color, sepal reflex, and flowering) that are full quadratic polynomials in PPF, DT, and NT. ROSESIM also incorporates mathematical models of nine plant growth characteristics (stem length and the following fresh and dry weights: stem, leaf, flower, and total) based on data recorded every 10 days and at flowering. At each design point, a cubic regression in time (days from pinch) estimated the plant growth characteristics on intermediate days; then difference equations were developed to predict the resulting daily growth increments as third-degree polynomial functions of days from pinch, PPF, DT, and NT. ROSESIM was verified by plotting against time each simulated plant growth characteristic and the associated experimental observations for the eight factorial design points defining the region of interest. Moreover, one-way analysis of variance procedures were applied to the differences between ROSESIM predictions and the corresponding observed means for all 15 treatment combinations. At 20 days from pinch, significant differences (P < 0.05) were observed for all nine plant growth characteristics. At 30 and 40 days from pinch, only flower fresh and dry weights yielded significant differences; at flowering, none of the 13

  17. Distribution of 45S rDNA in Modern Rose Cultivars (Rosa hybrida), Rosa rugosa, and Their Interspecific Hybrids Revealed by Fluorescence in situ Hybridization.

    Science.gov (United States)

    Ding, Xiao-Liu; Xu, Ting-Liang; Wang, Jing; Luo, Le; Yu, Chao; Dong, Gui-Min; Pan, Hui-Tang; Zhang, Qi-Xiang

    2016-01-01

    To elucidate the evolutionary dynamics of the location and number of rDNA loci in the process of polyploidization in the genus Rosa, we examined 45S rDNA sites in the chromosomes of 6 modern rose cultivars (R. hybrida), 5 R. rugosa cultivars, and 20 hybrid progenies by fluorescence in situ hybridization. Variation in the number of rDNA sites in parents and their interspecific hybrids was detected. As expected, 4 rDNA sites were observed in the genomes of 4 modern rose cultivars, while 3 hybridization sites were observed in the 2 others. Two expected rDNA sites were found in 2 R. rugosa cultivars, while in the other 3 R. rugosa cultivars 4 sites were present. Among the 20 R. hybrida × R. rugosa offspring, 13 carried the expected number of rDNA sites, and 1 had 6 hybridization sites, which exceeded the expected number by far. The other 6 offspring had either 2 or 3 hybridization sites, which was less than expected. Differences in the number of rDNA loci were observed in interspecific offspring, indicating that rDNA loci exhibit instability after distant hybridization events. Abnormal chromosome pairing may be the main factor explaining the variation in rDNA sites during polyploidization. © 2016 S. Karger AG, Basel.

  18. Influence of shading on ornamental and physiological characteristics during flower development of groundcover rose (Rosa hybrida L.)

    Science.gov (United States)

    Hou, Wei; Luo, Ya; Wang, Xiaorong; Chen, Qing; Sun, Bo; Wang, Yan; Liu, Zejing; Tang, Haoru; Zhang, Yong

    2018-04-01

    The objective of the present investigation was to study the effect of shading on flower quality during flower development and photosynthetic capacity of groundcover rose (Rosa hybrida L.). The results showed that shade significantly increased flower diameter, levels of soluble protein and soluble sugar, total carotenoids content and superoxide dismutase (SOD) activity, while contents of malondialdehyde (MDA) and total anthocyanins in shaded flowers were significantly decreased as compared to sun-exposed flowers. However, no significant changes were observed in petal color parameters L*, a*, b* and C* between sun exposure and shade treatment plants at each flower developmental stage. Therefore, groundcover rose seemed to have the capacity to shade condition through auto-regulation. These results could provide us with a theoretical basis for further application of groundcover rose in the greening of urban spaces and an understanding of the mechanisms behind the changes induced by shade.

  19. COMPORTAMIENTO FISIOLÓGICO POSTCOSECHA DE TALLOS FLORALES DE ROSA (Rosa hybrida L. EN RESPUESTA AL FÓSFORO APLICADO EN PRECOSECHA

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    Colinas-León MT

    2011-01-01

    Full Text Available Durante el crecimiento y desarro- llo de las flores, la nutrición fosforada es un factor que puede influir en la vida en flore- ro. El objetivo de este trabajo fue evaluar el efecto de cinco niveles de fósforo (P: 0, 0.5, 1.0, 1.5 y 2.0 me·L-1 de P aplicados en precosecha, mediante un sistema hidropóni- co abierto, en el comportamiento fisiológico postcosecha de tallos florales de rosa (Rosa hybrida L. ‘Classy’ y ‘Vega’. Las variables evaluadas durante la vida de florero de los tallos florales fueron: transpiración foliar y floral, potencial de turgencia foliar y de pétalos. Además, se evaluó la concentración de antocianinas en pétalos al momento de la cosecha. Las aplicaciones de P en precose- cha tuvieron efecto significativo únicamente en la transpiración foliar; sin embargo, se demostró que la mayor vida en florero (15.8 días de ‘Classy’ en comparación con ‘Vega’ (11.7 días estuvo directamente asociada con mayores potenciales de turgencia foliar y de pétalos, mayor tasa transpiratoria floral y menor tasa transpiratoria foliar en ‘Classy’.

  20. The Rose (Rosa hybrida) NAC Transcription Factor 3 Gene, RhNAC3, Involved in ABA Signaling Pathway Both in Rose and Arabidopsis

    OpenAIRE

    Jiang, Guimei; Jiang, Xinqiang; Lü, Peitao; Liu, Jitao; Gao, Junping; Zhang, Changqing

    2014-01-01

    Plant transcription factors involved in stress responses are generally classified by their involvement in either the abscisic acid (ABA)-dependent or the ABA-independent regulatory pathways. A stress-associated NAC gene from rose (Rosa hybrida), RhNAC3, was previously found to increase dehydration tolerance in both rose and Arabidopsis. However, the regulatory mechanism involved in RhNAC3 action is still not fully understood. In this study, we isolated and analyzed the upstream regulatory seq...

  1. Evaluating Susceptibility to Commercial Fungicide of Endophytic Fungi Isolated from Roses (Rosa hybrida

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    Ingrid Carolina Corredor Perilla

    2007-01-01

    Full Text Available Fungal endophytes have shown their potential as biocontrol agents; however, their application in commercial fields remains limited. Continuously applying fungicides to crops (specifically to roses may have harmful effects on endophyte growth. Endophytic fungi were isolated from R. hybrida and their susceptibility to fungicides regularly used for controlling important pathogens was analysed. This was performed in vitro, mixing several fungicide concentrations with standard medium for fungal endophytes; growth inhibition was then measured. The susceptibility of Botrytis cinerea (3015 strain, one of the most important pathogens affecting roses in Colombia, was also assessed using the same protocols. Active ingredients, such as boscalid, captan, iprodione and pyrimethanyl, showed susceptibility ranging from not sensitive (³73.75% to regularly sensitive (³48.75% - <61.25% for 45.45% of the fungal endophytes assessed. Endophytic fungi were highly susceptible to fungicides such as pyrimethanyl, carboxin plus thiram, fludioxonyl plus ciprodinyl and prochloraz. B. cinerea (3015 strain presented high susceptibility (<23.75% to fungicides such as pyrimethanyl, carboxin and thiram, fludioxonil and ciprodinyl, prochloraz. Although B. cinerea showed the greatest growth in controls, the endophytic fungi being assessed grew better in different media with fungicides. The results revealed some of these fungal endophytes’ potential for integrated pest management (IPM in roses in Colombia (3002, 3003, 3004, 3005 and 3006 strains, taking into account correct application time, application frequency and both fungal endophyte and fungicide dosage which may greatly limit fungal endophyte growth.

  2. Leaf Physiological and Proteomic Analysis to Elucidate Silicon Induced Adaptive Response under Salt Stress in Rosa hybrida 'Rock Fire'.

    Science.gov (United States)

    Soundararajan, Prabhakaran; Manivannan, Abinaya; Ko, Chung Ho; Muneer, Sowbiya; Jeong, Byoung Ryong

    2017-08-14

    Beneficial effects of silicon (Si) on growth and development have been witnessed in several plants. Nevertheless, studies on roses are merely reported. Therefore, the present investigation was carried out to illustrate the impact of Si on photosynthesis, antioxidant defense and leaf proteome of rose under salinity stress. In vitro-grown, acclimatized Rosa hybrida 'Rock Fire' were hydroponically treated with four treatments, such as control, Si (1.8 mM), NaCl (50 mM), and Si+NaCl. After 15 days, the consequences of salinity stress and the response of Si addition were analyzed. Scorching of leaf edges and stomatal damages occurred due to salt stress was ameliorated under Si supplementation. Similarly, reduction of gas exchange, photosynthetic pigments, higher lipid peroxidation rate, and accumulation of reactive oxygen species under salinity stress were mitigated in Si treatment. Lesser oxidative stress observed was correlated with the enhanced activity and expression of antioxidant enzymes, such as superoxide dismutase, catalase, and ascorbate peroxidase in Si+NaCl treatment. Importantly, sodium transportation was synergistically restricted with the stimulated counter-uptake of potassium in Si+NaCl treatment. Furthermore, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) results showed that out of 40 identified proteins, on comparison with control 34 proteins were down-accumulated and six proteins were up-accumulated due to salinity stress. Meanwhile, addition of Si with NaCl treatment enhanced the abundance of 30 proteins and downregulated five proteins. Differentially-expressed proteins were functionally classified into six groups, such as photosynthesis (22%), carbohydrate/energy metabolism (20%), transcription/translation (20%), stress/redox homeostasis (12%), ion binding (13%), and ubiquitination (8%). Hence, the findings reported in this work could facilitate a deeper

  3. A RhABF2/Ferritin module affects rose (Rosa hybrida) petal dehydration tolerance and senescence by modulating iron levels.

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    Liu, Jitao; Fan, Youwei; Zou, Jing; Fang, Yiqun; Wang, Linghao; Wang, Meng; Jiang, Xinqiang; Liu, Yiqing; Gao, Junping; Zhang, Changqing

    2017-12-01

    Plants often develop the capacity to tolerate moderate and reversible environmental stresses, such as drought, and to re-establish normal development once the stress has been removed. An example of this phenomenon is provided by cut rose (Rosa hybrida) flowers, which experience typical reversible dehydration stresses during post-harvest handling after harvesting at the bud stages. The molecular mechanisms involved in rose flower dehydration tolerance are not known, however. Here, we characterized a dehydration- and abscisic acid (ABA)-induced ferritin gene (RhFer1). Dehydration-induced free ferrous iron (Fe 2+ ) is preferentially sequestered by RhFer1 and not transported outside of the petal cells, to restrict oxidative stresses during dehydration. Free Fe 2+ accumulation resulted in more serious oxidative stresses and the induction of genes encoding antioxidant enzyme in RhFer1-silenced petals, and poorer dehydration tolerance was observed compared with tobacco rattle virus (TRV) controls. We also determined that RhABF2, an AREB/ABF transcription factor involved in the ABA signaling pathway, can activate RhFer1 expression by directly binding to its promoter. The silencing of RhABF2 decreased dehydration tolerance and disrupted Fe homeostasis in rose petals during dehydration, as did the silencing of RhFer1. Although both RhFer1 and Fe transporter genes are induced during flower natural senescence in plants, the silencing of RhABF2 or RhFer1 accelerates the petal senescence processes. These results suggest that the regulatory module RhABF2/RhFer1 contributes to the maintenance of Fe levels and enhances dehydration tolerance through the action of RhFer1 locally sequestering free Fe 2+ under dehydration conditions, and plays synergistic roles with transporter genes during flower senescence. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

  4. RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose (Rosa hybrida) petal senescence.

    Science.gov (United States)

    Lü, Peitao; Zhang, Changqing; Liu, Jitao; Liu, Xiaowei; Jiang, Guimei; Jiang, Xinqiang; Khan, Muhammad Ali; Wang, Liangsheng; Hong, Bo; Gao, Junping

    2014-05-01

    Rose (Rosa hybrida) is one of the most important ornamental plants worldwide; however, senescence of its petals terminates the ornamental value of the flower, resulting in major economic loss. It is known that the hormones abscisic acid (ABA) and ethylene promote petal senescence, while gibberellins (GAs) delay the process. However, the molecular mechanisms underlying the antagonistic effects amongst plant hormones during petal senescence are still unclear. Here we isolated RhHB1, a homeodomain-leucine zipper I transcription factor gene, from rose flowers. Quantitative RT-PCR and GUS reporter analyses showed that RhHB1 was strongly expressed in senescing petals, and its expression was induced by ABA or ethylene in petals. ABA or ethylene treatment clearly accelerated rose petal senescence, while application of the gibberellin GA3 delayed the process. However, silencing of RhHB1 delayed the ABA- or ethylene-mediated senescence, and resulted in higher petal anthocyanin levels and lower expression of RhSAG12. Moreover, treatment with paclobutrazol, an inhibitor of GA biosynthesis, repressed these delays. In addition, silencing of RhHB1 blocked the ABA- or ethylene-induced reduction in expression of the GA20 oxidase encoded by RhGA20ox1, a gene in the GA biosynthetic pathway. Furthermore, RhHB1 directly binds to the RhGA20ox1 promoter, and silencing of RhGA20ox1 promoted petal senescence. Eight senescence-related genes showed substantial differences in expression in petals after treatment with GA3 or paclobutrazol. These results suggest that RhHB1 mediates the antagonistic effect of GAs on ABA and ethylene during rose petal senescence, and that the promotion of petal senescence by ABA or ethylene operates through an RhHB1-RhGA20ox1 regulatory checkpoint. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.

  5. An autotetraploid linkage map of rose (Rosa hybrida) validated using the strawberry (Fragaria vesca) genome sequence.

    Science.gov (United States)

    Gar, Oron; Sargent, Daniel J; Tsai, Ching-Jung; Pleban, Tzili; Shalev, Gil; Byrne, David H; Zamir, Dani

    2011-01-01

    Polyploidy is a pivotal process in plant evolution as it increase gene redundancy and morphological intricacy but due to the complexity of polysomic inheritance we have only few genetic maps of autopolyploid organisms. A robust mapping framework is particularly important in polyploid crop species, rose included (2n = 4x = 28), where the objective is to study multiallelic interactions that control traits of value for plant breeding. From a cross between the garden, peach red and fragrant cultivar Fragrant Cloud (FC) and a cut-rose yellow cultivar Golden Gate (GG), we generated an autotetraploid GGFC mapping population consisting of 132 individuals. For the map we used 128 sequence-based markers, 141 AFLP, 86 SSR and three morphological markers. Seven linkage groups were resolved for FC (Total 632 cM) and GG (616 cM) which were validated by markers that segregated in both parents as well as the diploid integrated consensus map.The release of the Fragaria vesca genome, which also belongs to the Rosoideae, allowed us to place 70 rose sequenced markers on the seven strawberry pseudo-chromosomes. Synteny between Rosa and Fragaria was high with an estimated four major translocations and six inversions required to place the 17 non-collinear markers in the same order. Based on a verified linear order of the rose markers, we could further partition each of the parents into its four homologous groups, thus providing an essential framework to aid the sequencing of an autotetraploid genome.

  6. Non-linear mixed-effects modeling for photosynthetic response of Rosa hybrida L. under elevated CO2 in greenhouses - Short communication

    DEFF Research Database (Denmark)

    Öztürk, I.; Ottosen, C.O.; Ritz, C.

    2011-01-01

    Photosynthetic response to light was measured on the leaves of two cultivars of Rosa hybrida L. (Escimo and Mercedes) in the greenhouse to obtain light-response curves and their parameters. Th e aim was to use a model to simulate leaf photosynthetic carbon gain with respect to environmental condi...

  7. Uso eficiente del agua para el cultivo de rosa cv. Freedom bajo invernadero

    Directory of Open Access Journals (Sweden)

    John J. Arévalo-H

    2013-08-01

    Full Text Available Determinar el requerimiento de agua y el momento adecuado de distribución, es un factor fundamental para el cultivo de flores. El objetivo del presente trabajo fue determinar el efecto de la aplicación de tres láminas de riego sobre el desarrollo y producción del cultivo de la Rosa cv. Freedom, bajo invernadero. El experimento se realizó con tres láminas de riego en un cultivo de rosa (Rosa spp. cv. Freedom. Se analizó el crecimiento de los tallos florales, la vida en florero, la producción y la eficiencia en el uso del agua. Los tratamientos se basaron en tres niveles de evapotranspiración de cultivo (ETc (70, 80 y 100%. Durante el estudio, el cultivo se mantuvo en producción constante. Se aplicaron 384,87, 254,25 y 218,97 m³ correspondiente a 100, 80 e 70% de ETc respectivamente, significando un ahorro de agua de 14,0 y 21,8% en el 80 e 70% de ETc respectivamente. El coeficiente de cultivo (Kc vario entre 0,58 y 1,65, con una media de 1,13. No se encontraron diferencias en la producción. Las curvas de crecimiento presentaron incremento continuo, con ajuste al modelo logístico. El 70% de ETc presentó la mayor eficiencia en el uso del agua, con 185 tallos m-3. La conservación media de los tallos florales fue de 12 a 15 días. La mayor causa de pérdida fue la deshidratación, que inició en el día 11, comportamiento normal del tallo floral.

  8. The Effects of IBA and NAA, and Rooting Media on Propagation of Miniature Rose Cuttings (Rosa hybrida

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    azam ranjbar

    2017-09-01

    Full Text Available Introduction: Miniature rose (Rosa hybrida are well known as one of the world’s most popular ornamental plants cultivated worldwide as potted and/or bed plants. Nowadays, more than 100 million pots of miniature roses are propagated by stem cutting in the commercial greenhouses of European countries such as Denmark and Germany. Some treatments such as application of plant growth regulators and suitable rooting medium could be required for accelerating root formation in rose cuttings. Using plant growth regulators like natural or synthetic auxin is a pre-requirement for the initiation of adventitious root in some stem cuttings and it has been reported that the division of the first initiator cells of root depends on internal or synthetic auxin. Methods of application of these chemicals and suitable concentration could be related to several factors, importantly the plant varieties, type of cuttings and the time of cutting preparation. Various kinds of media such as soil, peat moss, perlite and vermiculite are used as bed substrate according to required ratio. Rooting media must provide appropriate moisture and air ventilation for cuttings establishment, which highly affect the cuttings root formation. Appropriate procedure for using wastes materials as culture bed, especially those materials that produced locally, is main aim of some studies to find an alternative medium in ornamental pot plant production. In this regards, evaluation of agricultural wastes to be used to culture bed and introducing suitable materials could be considered. Accordingly, the objective of the present study was to determine the effects of two types of plant growth regulators and bed combinations on rooting percentage of semi-hardwood cuttings in miniature rose. Materials and Methods: In order to evaluate the effects of different concentrations of indolebutyric acid (IBA and naphtaleneacetic acid (NAA, and two media with different composition on root formation of

  9. PhDAHP1 is required for floral volatile benzenoid/phenylpropanoid biosynthesis in Petunia × hybrida cv 'Mitchell Diploid'.

    Science.gov (United States)

    Langer, Kelly M; Jones, Correy R; Jaworski, Elizabeth A; Rushing, Gabrielle V; Kim, Joo Young; Clark, David G; Colquhoun, Thomas A

    2014-07-01

    Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis consists of numerous enzymatic and regulatory processes. The initial enzymatic step bridging primary metabolism to secondary metabolism is the condensation of phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) carried out via 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE (DAHP) synthase. Here, identified, cloned, localized, and functionally characterized were two DAHP synthases from the model plant species Petunia × hybrida cv 'Mitchell Diploid' (MD). Full-length transcript sequences for PhDAHP1 and PhDAHP2 were identified and cloned using cDNA SMART libraries constructed from pooled MD corolla and leaf total RNA. Predicted amino acid sequence of PhDAHP1 and PhDAHP2 proteins were 76% and 80% identical to AtDAHP1 and AtDAHP2 from Arabidopsis, respectively. PhDAHP1 transcript accumulated to relatively highest levels in petal limb and tube tissues, while PhDAHP2 accumulated to highest levels in leaf and stem tissues. Through floral development, PhDAHP1 transcript accumulated to highest levels during open flower stages, and PhDAHP2 transcript remained constitutive throughout. Radiolabeled PhDAHP1 and PhDAHP2 proteins localized to plastids, however, PhDAHP2 localization appeared less efficient. PhDAHP1 RNAi knockdown petunia lines were reduced in total FVBP emission compared to MD, while PhDAHP2 RNAi lines emitted 'wildtype' FVBP levels. These results demonstrate that PhDAHP1 is the principal DAHP synthase protein responsible for the coupling of metabolites from primary metabolism to secondary metabolism, and the ultimate biosynthesis of FVBPs in the MD flower. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Localisation of Abundant and Organ-Specific Genes Expressed in Rosa hybrida Leaves and Flower Buds by Direct In Situ RT-PCR

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    Agata Jedrzejuk

    2012-01-01

    Full Text Available In situ PCR is a technique that allows specific nucleic acid sequences to be detected in individual cells and tissues. In situ PCR and IS-RT-PCR are elegant techniques that can increase both sensitivity and throughput, but they are, at best, only semiquantitative; therefore, it is desirable first to ascertain the expression pattern by conventional means to establish the suitable conditions for each probe. In plants, in situ RT-PCR is widely used in the expression localisation of specific genes, including MADS-box and other function-specific genes or housekeeping genes in floral buds and other organs. This method is especially useful in small organs or during early developmental stages when the separation of particular parts is impossible. In this paper, we compared three different labelling and immunodetection methods by using in situ RT-PCR in Rosa hybrida flower buds and leaves. As target genes, we used the abundant β-actin and RhFUL gene, which is expressed only in the leaves and petals/sepals of flower buds. We used digoxygenin-11-dUTP, biotin-11-dUTP, and fluorescein-12-dUTP-labelled nucleotides and antidig-AP/ streptavidin-fluorescein-labelled antibodies. All of the used methods gave strong, specific signal and all of them may be used in localization of gene expression on tissue level in rose organs.

  11. Efecto de tres preservadores de la longevidad sobre la vida postcosecha de Rosa cv. Royalty

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    G. H. De la Cruz-Guzmán

    2007-01-01

    Full Text Available Se evaluó el efecto de tres tratamientos: a sulfato de aluminio 0.6 g·l-1; b 400 ppm de sulfato de 8-hidroxiquinoleina (8-HQS; c pulso con tiosulfato de plata (STS, 0.025 mM durante 20 minutos y agua destilada como testigo sobre consumo hídrico, peso fresco, diámetro floral, concentración de azúcares solubles totales en pétalos y longevidad en Rosa cv. Royalty. En el testigo el consumo de agua descendió significativamente al noveno día, no así en los otros tratamientos que se mantuvieron por arriba del testigo hasta el final del experimento. La menor tasa de pérdida de peso fresco se observó en el tratamiento con sulfato de aluminio (Al, seguido por STS, HQS y testigo respectivamente, sugiriendo una probable asociación inversa con la vida postcosecha. Las flores tratadas con sulfato de aluminio, resultaron estadísticamente diferentes en cuanto a su estado ornamental óptimo en los días 9, 10, 11, 12 y 14 de vida postcosecha, evaluados mediante la prueba de Kruskall-Wallis El diámetro floral fue mayor en las flores tratadas con STS (9 cm, seguido por sulfato de aluminio (7.6 cm; éste último mantuvo la apertura dos días más que STS. En cuanto a la concentración de azúcares solubles totales en estado de botón y flor abierta, no se registraron diferencias significativas (P>0.05 ni entre tratamientos ni estados florales.

  12. Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida).

    Science.gov (United States)

    Ma, Nan; Chen, Wen; Fan, Tiangang; Tian, Yaran; Zhang, Shuai; Zeng, Daxing; Li, Yonghong

    2015-10-05

    Flower development is central to angiosperm reproduction and is regulated by a broad range of endogenous and exogenous stimuli. It has been well documented that ambient temperature plays a key role in controlling flowering time; however, the mechanisms by which temperature regulates floral organ differentiation remain largely unknown. In this study, we show that low temperature treatment significantly increases petal number in rose (Rosa hybrida) through the promotion of stamen petaloidy. Quantitative RT-PCR analysis revealed that the expression pattern of RhAG, a rose homolog of the Arabidopsis thaliana AGAMOUS C-function gene, is associated with low temperature regulated flower development. Silencing of RhAG mimicked the impact of low temperature treatments on petal development by significantly increasing petal number through an increased production of petaloid stamens. In situ hybridization studies further revealed that low temperature restricts its spatial expression area. Analysis of DNA methylation level showed that low temperature treatment enhances the methylation level of the RhAG promoter, and a specific promoter region that was hypermethylated at CHH loci under low temperature conditions, was identified by bisulfite sequencing. This suggests that epigenetic DNA methylation contributes to the ambient temperature modulation of RhAG expression. Our results provide highlights in the role of RhAG gene in petal number determination and add a new layer of complexity in the regulation of floral organ development. We propose that RhAG plays an essential role in rose flower patterning by regulating petal development, and that low temperatures increase petal number, at least in part, by suppressing RhAG expression via enhancing DNA CHH hypermethylation of the RhAG promoter.

  13. Leaf Physiological and Proteomic Analysis to Elucidate Silicon Induced Adaptive Response under Salt Stress in Rosa hybrida ‘Rock Fire’

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    Prabhakaran Soundararajan

    2017-08-01

    Full Text Available Beneficial effects of silicon (Si on growth and development have been witnessed in several plants. Nevertheless, studies on roses are merely reported. Therefore, the present investigation was carried out to illustrate the impact of Si on photosynthesis, antioxidant defense and leaf proteome of rose under salinity stress. In vitro-grown, acclimatized Rosa hybrida ‘Rock Fire’ were hydroponically treated with four treatments, such as control, Si (1.8 mM, NaCl (50 mM, and Si+NaCl. After 15 days, the consequences of salinity stress and the response of Si addition were analyzed. Scorching of leaf edges and stomatal damages occurred due to salt stress was ameliorated under Si supplementation. Similarly, reduction of gas exchange, photosynthetic pigments, higher lipid peroxidation rate, and accumulation of reactive oxygen species under salinity stress were mitigated in Si treatment. Lesser oxidative stress observed was correlated with the enhanced activity and expression of antioxidant enzymes, such as superoxide dismutase, catalase, and ascorbate peroxidase in Si+NaCl treatment. Importantly, sodium transportation was synergistically restricted with the stimulated counter-uptake of potassium in Si+NaCl treatment. Furthermore, two-dimensional electrophoresis (2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS results showed that out of 40 identified proteins, on comparison with control 34 proteins were down-accumulated and six proteins were up-accumulated due to salinity stress. Meanwhile, addition of Si with NaCl treatment enhanced the abundance of 30 proteins and downregulated five proteins. Differentially-expressed proteins were functionally classified into six groups, such as photosynthesis (22%, carbohydrate/energy metabolism (20%, transcription/translation (20%, stress/redox homeostasis (12%, ion binding (13%, and ubiquitination (8%. Hence, the findings reported in this work could facilitate a

  14. The rose (Rosa hybrida) NAC transcription factor 3 gene, RhNAC3, involved in ABA signaling pathway both in rose and Arabidopsis.

    Science.gov (United States)

    Jiang, Guimei; Jiang, Xinqiang; Lü, Peitao; Liu, Jitao; Gao, Junping; Zhang, Changqing

    2014-01-01

    Plant transcription factors involved in stress responses are generally classified by their involvement in either the abscisic acid (ABA)-dependent or the ABA-independent regulatory pathways. A stress-associated NAC gene from rose (Rosa hybrida), RhNAC3, was previously found to increase dehydration tolerance in both rose and Arabidopsis. However, the regulatory mechanism involved in RhNAC3 action is still not fully understood. In this study, we isolated and analyzed the upstream regulatory sequence of RhNAC3 and found many stress-related cis-elements to be present in the promoter, with five ABA-responsive element (ABRE) motifs being of particular interest. Characterization of Arabidopsis thaliana plants transformed with the putative RhNAC3 promoter sequence fused to the β-glucuronidase (GUS) reporter gene revealed that RhNAC3 is expressed at high basal levels in leaf guard cells and in vascular tissues. Moreover, the ABRE motifs in the RhNAC3 promoter were observed to have a cumulative effect on the transcriptional activity of this gene both in the presence and absence of exogenous ABA. Overexpression of RhNAC3 in A. thaliana resulted in ABA hypersensitivity during seed germination and promoted leaf closure after ABA or drought treatments. Additionally, the expression of 11 ABA-responsive genes was induced to a greater degree by dehydration in the transgenic plants overexpressing RhNAC3 than control lines transformed with the vector alone. Further analysis revealed that all these genes contain NAC binding cis-elements in their promoter regions, and RhNAC3 was found to partially bind to these putative NAC recognition sites. We further found that of 219 A. thaliana genes previously shown by microarray analysis to be regulated by heterologous overexpression RhNAC3, 85 are responsive to ABA. In rose, the expression of genes downstream of the ABA-signaling pathways was also repressed in RhNAC3-silenced petals. Taken together, we propose that the rose RhNAC3 protein

  15. The rose (Rosa hybrida NAC transcription factor 3 gene, RhNAC3, involved in ABA signaling pathway both in rose and Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Guimei Jiang

    Full Text Available Plant transcription factors involved in stress responses are generally classified by their involvement in either the abscisic acid (ABA-dependent or the ABA-independent regulatory pathways. A stress-associated NAC gene from rose (Rosa hybrida, RhNAC3, was previously found to increase dehydration tolerance in both rose and Arabidopsis. However, the regulatory mechanism involved in RhNAC3 action is still not fully understood. In this study, we isolated and analyzed the upstream regulatory sequence of RhNAC3 and found many stress-related cis-elements to be present in the promoter, with five ABA-responsive element (ABRE motifs being of particular interest. Characterization of Arabidopsis thaliana plants transformed with the putative RhNAC3 promoter sequence fused to the β-glucuronidase (GUS reporter gene revealed that RhNAC3 is expressed at high basal levels in leaf guard cells and in vascular tissues. Moreover, the ABRE motifs in the RhNAC3 promoter were observed to have a cumulative effect on the transcriptional activity of this gene both in the presence and absence of exogenous ABA. Overexpression of RhNAC3 in A. thaliana resulted in ABA hypersensitivity during seed germination and promoted leaf closure after ABA or drought treatments. Additionally, the expression of 11 ABA-responsive genes was induced to a greater degree by dehydration in the transgenic plants overexpressing RhNAC3 than control lines transformed with the vector alone. Further analysis revealed that all these genes contain NAC binding cis-elements in their promoter regions, and RhNAC3 was found to partially bind to these putative NAC recognition sites. We further found that of 219 A. thaliana genes previously shown by microarray analysis to be regulated by heterologous overexpression RhNAC3, 85 are responsive to ABA. In rose, the expression of genes downstream of the ABA-signaling pathways was also repressed in RhNAC3-silenced petals. Taken together, we propose that the rose Rh

  16. Non-linear mixed-effects modeling for photosynhetic response of Rosa hybrida L. under elevated CO2 in greenhouses - short communication

    DEFF Research Database (Denmark)

    Ozturk, I.; Ottosen, C.O.; Ritz, Christian

    2011-01-01

    conditions. Leaf gas exchanges were measured at 11 light intensities from 0 to 1,400 µmol/m2s, at 800 ppm CO2, 25°C, and 65 ± 5% relative humidity. In order to describe the data corresponding to diff erent measurement dates, the non-linear mixed-eff ects regression analysis was used. Th e model successfully...... effi ciency. Th e results suggested acclimation response, as carbon assimilation rates and stomatal conductance at each measurement date were higher for Escimo than Mercedes. Diff erences in photosynthesis rates were attributed to the adaptive capacity of the cultivars to light conditions at a specifi......Photosynthetic response to light was measured on the leaves of two cultivars of Rosa hybrida L. (Escimo and Mercedes) in the greenhouse to obtain light-response curves and their parameters. Th e aim was to use a model to simulate leaf photosynthetic carbon gain with respect to environmental...

  17. Pollination increases ethylene production in Lilium hybrida cv. Brindisi flowers but does not affect the time to tepal senescence or tepal abscission.

    Science.gov (United States)

    Pacifici, Silvia; Prisa, Domenico; Burchi, Gianluca; van Doorn, Wouter G

    2015-01-15

    In many species, pollination induces a rapid increase in ethylene production, which induces early petal senescence, petal abscission, or flower closure. Cross-pollination in Lilium hybrida cv. Brindisi resulted in a small increase in flower ethylene production. In intact plants and in isolated flowers, pollination had no effect on the time to tepal senescence or tepal abscission. When applied to closed buds of unpollinated flowers, exogenous ethylene slightly hastened the time to tepal senescence and abscission. However, exogenous ethylene had no effect when the flowers had just opened, i.e. at the time of pollination. Experiments with silver thiosulphate, which blocks the ethylene receptor, indicated that endogenous ethylene had a slight effect on the regulation of tepal senescence and tepal abscission, although only at the time the tepals were still inside buds and not in open flowers. Low ethylene-sensitivity after anthesis therefore explains why pollination had no effect on the processes studied. Copyright © 2014 Elsevier GmbH. All rights reserved.

  18. The Study of Controlling the Bacterial Growth in the Cut Stem Rose (Rosa Hybrida L.‘Dolce Vita’ Flowers with Preservative Compounds

    Directory of Open Access Journals (Sweden)

    A. Oraee

    2015-01-01

    Full Text Available Due to the economic importance of longevity of cut flowers, this study was conducted to evaluate the effect of nano-silver, silver thiosulfate, hydroxyl quinoline and a natural compound, thymol, on vase life and number of bacteria in cut stem ends of Rose flowers cv. Dolce vita. Cut flowers were transferred to laboratory at 22 ± 1ºC temperature and 60 ± 5% relative humidity. Cut flowers were pulse-treated with 2.5, 5 and 10 mg L-1 nano-silver, 0.5,1 and 2 mM silver thiosulfate, 50, 100 and 200 mg L-1 hydroxy quinoline and 50, 100, 200 mg L-1 thymol with 5% sucrose. Experiment was conducted in a completely randomized design with 8 replications and one flower in each experimental unit. Based on the results, all of the treatments showed positive effects on the vase life of flowers and number of bacteria in cut stem ends during the vase period, and no bacteria were found in 5 and 10 mg L-1 SNP treatments. The nano-silver (5 and 10 mg L-1 treatments showed the greatest mean longevity (19 days. Considering the results and economical and functional aspects of the tested materials, nano-silver treatment can be used for increasing the vase life of ‘Dolce vita’rose.

  19. Evaluación del Fertilizante Orgánico Líquido de Lombriz San Rafael en el Cultivo de Rosa cv. Classy Evaluation of the Worm Organic Liquid Fertilizer San Rafael on cv. Classy Rose Crop

    Directory of Open Access Journals (Sweden)

    Fernando Ramírez Castañeda

    2011-12-01

    Full Text Available La aplicación de fertilizantes es una de las principales actividades técnicas que se realizan en el cultivo de rosas, práctica que puede llegar a causar un impacto ambiental importante. El uso de fertilizantes orgánicos en este cultivo ha sido mínimo. Fertilizantes orgánicos como los vermicompuestos aportan elementos, algunos en formas más disponibles, como también mejoran las características físicas de los suelos. El objetivo fue evaluar la adición del fertilizante orgánico líquido de lombriz San Rafael a la fertilización comercial completa y la reducción de un 25% de su composición por cama, midiendo su efecto semanalmente sobre la productividad y la calidad de los tallos florales, el descabece de flor y en análisis foliares en plantas de rosa cv. Classy. Los resultados indican que para los análisis foliares y los diferentes grados de calidad (longitud de tallo y de botón floral no existió evidencia de diferencias estadísticas entre tratamientos. Se encontró diferencia significativa en productividad, siendo mayor el promedio de las plantas tratadas con la fórmula de fertilización completa más el fertilizante orgánico. Para el descabece de la flor se encontró que el tratamiento donde se aplicó la fórmula de fertilización reducida más el fertilizante orgánico, fue significativamente menor en comparación con los otros dos tratamientos.Fertilizer application is one of the main technical activities made in rose crop, but it may cause an important environmental impact. The use of organic fertilizers has been low in this crop. An organic fertilizer as vermicompost contributes with some elements in better available forms, and improves some soil physical characteristics. The purpose was to evaluate the addition of worm liquid organic fertilizer San Rafael to complete commercial fertilization and to decrease 25% of its composition per bed, measuring weekly its effect on yield, degrees of quality flowering stems

  20. Influência do 1-MCP na conservação pós-colheita de rosas cv. Vega Influence of 1-MCP on postharvest conservation of roses cv. Vega

    Directory of Open Access Journals (Sweden)

    Júlia De Pietro

    2010-10-01

    Full Text Available Objetivou-se, neste trabalho, estudar aspectos da fisiologia pós-colheita de rosas cortadas cv. Vega, tratadas com diferentes concentrações de 1-metilcicloropropeno (1-MCP. O experimento foi conduzido em delineamento inteiramente casualizado, em esquema fatorial composto por dois fatores: quatro tratamentos pós-colheita e quatro datas de avaliação (0; 4; 8; 12 dias. As flores foram submetidas aos seguintes tratamentos: 1 Água destilada (controle; 2 1-MCP (100 ppb; 3 1-MCP (250 ppb; 4 1-MCP (500 ppb. Foram realizadas as seguintes avaliações: massa fresca, cor, turgescência, curvatura, taxa respiratória, conteúdo relativo de água, conteúdo de carboidratos solúveis, de redutores e de antocianina. Ao contrário do tratamento controle, todas as concentrações de 1-MCP foram eficientes para retardar a senescência das flores, porém, a concentração de 1-MCP a 500 ppb destacou-se pela melhor manutenção da qualidade, além de prolongar a vida decorativa das hastes até 19 dias.The objective of this work was to study aspects of postharvest physiology of cut roses cv. Vega, treated with different concentrations of 1-metilcloropropene (1-MCP. The experiment was conducted in a complete randomized design composed of two factors: four postharvest treatments and four dates of evaluation (0; 4; 8; 12 days. The following treatments were used: 1 distilled water; 2 1-metilciclopropeno (1-MCP 100 ppb; 3 1-MCP 250 ppb; 4 1-MCP 500 ppb. The following evaluations were made: fresh weight, color, turgor, curvature, respiration rate, relative water content, content of soluble carbohydrates, reducing and anthocyanin. Unlike treatment with distilled water, all concentrations of 1-MCP were effective in delaying the senescence of flowers; however, the concentration of 1-MCP at 500 ppb was highlighted by better maintenance of quality and extended decorate life stem up to 19 days.

  1. Analysis of metabolome effects of hop (H. lupulus) transcription factors in heterologous of Petunia hybrida

    OpenAIRE

    MORAVCOVÁ, Vendula

    2013-01-01

    Plant transformation is now a key research in plant biology, and also is a unique practical tool in the improvement of varieties of cultivated plants. The aim of this work was to obtain transgenic plants Petunia x hybrida containing transgene nptII (kanamycin resistance gene) using the indirect transformation by Agrobacterium tumefaciens. In terms of in-vivo was grown from seeds experimental material in the form of plant Petunia x hybrida cv. Andrea. For the experiments three different constr...

  2. Rosa red, Rosa eagle: simply Rosa Luxemburgo

    Directory of Open Access Journals (Sweden)

    Beatriz Rajland

    2010-10-01

    Full Text Available Rosa Luxemburgo was a complete revolutionary in her theoretical production and action. An internationalist, she was a communist in action and in participatory thinking, who was dedicated to defending working class interests, with a constant concerned for liberty, democracy, complete development and for fighting bureaucracy. She was distinguished for not accepting traditional formulas, while remaining very rigorous with herself, never ceasing to reformulate her ideas and actions, which in her understanding, had a special value in a liberating political struggle. Today, the ?battle of ideas? acquires a very important place in Latin America and the Caribbean, as basic support in the articulation of the social and political realms, and the construction of a future of emancipation, of which Rosa Luxemburgo was a pioneer.

  3. Rosa limensis

    OpenAIRE

    Mujica Puntilla, Ramón; Brading, David A.; Saranyana, Josep Ignasi

    2013-01-01

    Rosa limensis es un ensayo interdisciplinario de historia cultural que resalta los orígenes religiosos del nacionalismo peruano. Basaso en los expedientes inéditos del proceso ordinario (1617-1618) y apostólico (1630-1632) de beatificación y canonizacíon, el libro analiza el universo místico y visionario de santa Rosa de Lima (1586-1617), la primera santa america. Pese a haber sido cuestionada por la Inquisición limeña e identificada con las herterodoxias alumbradistas españolas, aquí se rast...

  4. Petunia (Petunia hybrida).

    Science.gov (United States)

    Lutke, W Kevin

    2006-01-01

    Petunia hybrida genetic transformation continues to be a valuable tool for genetic research into biochemical pathways and gene expression, as well as generating commercial products with varying floral colors. In this chapter, we describe a simple and reproducible genetic transformation protocol for generating transgenic petunia plants harboring a gene of interest and selectable marker. The system utilizes Agrobacterium tumefaciens for transgene integration with plant recovery via shoot organogenesis from leaf explant material. Selection for transgenic plants is achieved using the bar gene conferring resistance to glufosinate or nptII gene for resistance to kanamycin. Transformation efficiencies of around 10% are achievable with shoots being recovered about 8 wk after transgene insertion and rooted plants transferred to the greenhouse about twelve weeks after inoculation.

  5. Expression analysis of dihydroflavonol 4-reductase genes in Petunia hybrida.

    Science.gov (United States)

    Chu, Y X; Chen, H R; Wu, A Z; Cai, R; Pan, J S

    2015-05-12

    Dihydroflavonol 4-reductase (DFR) genes from Rosa chinensis (Asn type) and Calibrachoa hybrida (Asp type), driven by a CaMV 35S promoter, were integrated into the petunia (Petunia hybrida) cultivar 9702. Exogenous DFR gene expression characteristics were similar to flower-color changes, and effects on anthocyanin concentration were observed in both types of DFR gene transformants. Expression analysis showed that exogenous DFR genes were expressed in all of the tissues, but the expression levels were significantly different. However, both of them exhibited a high expression level in petals that were starting to open. The introgression of DFR genes may significantly change DFR enzyme activity. Anthocyanin ultra-performance liquid chromatography results showed that anthocyanin concentrations changed according to DFR enzyme activity. Therefore, the change in flower color was probably the result of a DFR enzyme change. Pelargonidin 3-O-glucoside was found in two different transgenic petunias, indicating that both CaDFR and RoDFR could catalyze dihydrokaempferol. Our results also suggest that transgenic petunias with DFR gene of Asp type could biosynthesize pelargonidin 3-O-glucoside.

  6. Rosa Blanca

    Directory of Open Access Journals (Sweden)

    Gloria Lapeña-Gallego

    2017-01-01

    Full Text Available A través de la Memoria Histórica, la Historia con mayúsculas se ha convertido en una fuente inagotable para narrar grandes historias con minúscula. De manera paralela, el paso de una cultura eminentemente letrada a otra más visual con predominio de la imagen ha favorecido la progresiva incorporación en el ámbito educativo de fuentes procedentes de la cultura popular, cuyos autores se aproximan al hecho histórico desde una perspectiva más íntima y reflexiva. En el presente artículo se fundamenta el potencial del álbum ilustrado como fórmula narrativa complementaria al manual escolar o libro de texto de Historia, especialmente en el tratamiento de temas complejos y delicados, como el holocausto nazi. Además, se propone un modelo de análisis que tiene en cuenta las particularidades de este género artístico en la construcción de la historiografía por medio de la incorporación de imágenes que van más allá de ser una redundancia o aclaración del texto. Para el cumplimiento de ambos objetivos, una vez analizado la concepción actual de la narración histórica, se estudia en detalle el álbum Rosa Blanca, contextualizado en la ocupación nazi durante la Segunda Guerra Mundial.

  7. Sensibilidade da rosa 'Osiana' ao etileno

    Directory of Open Access Journals (Sweden)

    Delaine Cristina Cordeiro

    2011-01-01

    Full Text Available Os objetivos deste trabalho foram determinar a sensibilidade e as respostas da rosa cultivar Osiana (Rosa × hybrida à presença de etileno ao longo da vida pós-colheita. Para determinar a sensibilidade da flor ao etileno, hastes foram cortadas e colocadas em câmaras herméticas e em seguida tratadas por 24 horas com diferentes concentrações de etileno variando de 0,0; 0,1; 1,0; 10; 100 e 1000 µL L-1do ar no interior da câmara. A rosa teve a vida pós-colheita reduzida pelo etileno, quando exposta a concentração igual ou superior a 10 µL L-1 de etileno. As flores tratadas com no mínimo de 10 µL L-1de etileno desenvolveram necrose, murcha e abscisão prematura das pétalas ainda túrgidas. As concentrações de 0,1 e 1,0 µL L-1de etileno estimularam a abertura do botão floral durante o período de 24 horas de exposição ao etileno, sem no entanto, reduzir a vida pós-colheita. A rosa 'Osiana' pode ser classificada como uma cultivar sensível ao etileno e a concentração influência os processos estimulados.

  8. Produção e qualidade de rosas em razão de doses de boro aplicadas no substrato

    Directory of Open Access Journals (Sweden)

    Luiz Antônio Zanão Júnior

    2014-04-01

    Full Text Available Muitos trabalhos têm demonstrado os efeitos da aplicação do boro (B em variadas culturas; entretanto, poucos registros demonstram seus efeitos na produção de rosas. Objetivou-se com este experimento avaliar os efeitos de doses de B na produção e qualidade de rosas (Rosa hybrida cv. Shiny Terrazza® em vaso. Os tratamentos foram cinco doses de B (0, 1, 4, 8 e 16 mg kg-1, aplicadas no substrato, em delineamento de blocos casualizados, com cinco repetições. Foram avaliados: número de flores por planta; número de folhas por haste floral; produção de matéria seca de raízes, folhas e flores; altura da planta; número de dias para o florescimento; comprimento do botão floral; e diâmetro e longevidade floral. Além disso, foram determinados os teores foliares de clorofila total e B e os sintomas de toxidez desse elemento. Verificou-se efeito significativo das doses de B na maioria das variáveis avaliadas, excetuando-se a altura das plantas, o número de flores por planta, a longevidade floral e a produção de matéria seca de raízes. O teor foliar de B aumentou linearmente em função das doses desse elemento. Houve incremento na produção e qualidade das flores com a aplicação do B, com redução do número de dias para o florescimento. Foram verificados sintomas de toxidez causado pelo B a partir da dose de 4 mg kg-1. Esses sintomas foram caracterizados por manchas do tipo encharcamento, iniciando nas margens do limbo foliar, com essas evoluindo para clorose e posterior necrose; na maior dose ocorreu queda de folhas. Essa queda foi responsável pela diminuição do número de folhas por haste no final do ciclo, a partir da dose de 8 mg kg-1 de B. As plantas com sintomas de toxidez apresentaram teores foliares de B acima de 200 mg kg-1, enquanto as sadias (controle, de 65 a 89 mg kg-1.

  9. Rosa L.: rose, briar

    Science.gov (United States)

    Susan E. Meyer

    2008-01-01

    The genus Rosa is found primarily in the North Temperate Zone and includes about 200 species, with perhaps 20 that are native to the United States (table 1). Another 12 to 15 rose species have been introduced for horticultural purposes and are naturalized to varying degrees. The nomenclature of the genus is in a state of flux, making it difficult to number the species...

  10. Study Of Phytohormone Autonomy In In Vitro In Petunia Hybrida

    OpenAIRE

    S. Sharmila Begum; A. Arundhati

    2016-01-01

    Petunia x hybrida is used as noble genetic material for somatic hybridization, molecular studies and hence is of concern in phylogenetic significance to serve as a useful models of physiology and host - parasite interaction. In the present study using two variants of Petunia hybrida, the phytohormone autonomy on different formulation of MS medium was carried out. The type of response on hormone free MS medium was rhizogenesis in both the variants. When hormones IAA was substituted...

  11. Introduction of ROL genes in Rosa Hybrida L. for improved rootstock performance

    Energy Technology Data Exchange (ETDEWEB)

    Van der Salm, T.P.M.

    1996-06-04

    We aim to improve the performance of combination plants of rose by genetic modification of its root stock, with special reference to their performance at low energy conditions. The first objective of this research was the introduction of the ROL genes from A. rhizogenes in the recalcitrant species rose. For this purpose the rose root stock `Money way` was used. The second objective was to determine the effects of these genes on the rooting characteristics of root stock plants and their influence on the growth performance of combination plants. Special attention had to be paid to methodological aspects such as micropropagation, transformation and regeneration. First, the medium composition was optimized for the in vitro culture of root stock `Money way`. The effect of ROL genes on the formation of adventitious roots was described. It was shown that the formation of kanamycin resistant roots was stimulated by ROL genes, especially by a combination of ROLA, B and C. A regeneration method was developed, in which shoots were regenerated from excised roots via somatic embryogenesis. This regeneration method was applied for the regeneration of transformed shoots from kanamycin resistant roots, especially from those with the ROLB gene or a combination of ROLA, B and C genes. As a result several transformed plants were obtained. Expression patterns of the ROL genes were correlated with the phenotypical characteristics of the transformed rose plants. In order to determine the effect of ROL genes on the growth and development of a scion, combination plants were made, consisting of the root system of a transgenic root stock and the aerial parts of the cultivar `Madelon`. For evaluation of the effects of ROL genes on root stock performance and energy efficiency, the combination plants were studied under climate room conditions at 15 and 20 deg C. Finally, an overview is given of the possible applications of ROL genes for crop improvement.

  12. Proteomic Analysis of Aphid-Resistant and -Sensitive Rose (Rosa Hybrida) Cultivars at Two Developmental Stages.

    Science.gov (United States)

    Muneer, Sowbiya; Jeong, Hai Kyoung; Park, Yoo Gyeong; Jeong, Byoung Ryong

    2018-05-25

    The rose is one the most commercially grown and costly ornamental plants because of its aesthetic beauty and aroma. A large number of pests attack its buds, flowers, leaves, and stem at every growing stage due to its high sugar content. The most common pest on roses are aphids which are considered to be the major cause for product loss. Aphid infestations lead to major changes in rose plants, such as large and irregular holes in petals, intact leaves and devouring tissues. It is hypothesized that different cut rose cultivars would have different levels of sensitivity or resistance to aphids, since different levels of infestation are observed in commercially cut rose production greenhouses. The present work compared four cut rose cultivars which were bred in Korea and were either resistant or sensitive to aphid infestation at different flower developmental stages. An integrative study was conducted using comprehensive proteome analyses. Proteins related to ubiquitin metabolism and the stress response were differentially expressed due to aphid infestation. The regulations and possible functions of identified proteins are presented in detail. The differential expressions of the identified proteins were validated by immunoblotting and blue native page. In addition, total sugar and carbohydrate content were also observed.

  13. Rose (Rosa hybrida L.) tissue culture mutagenesis for new mutants generation

    International Nuclear Information System (INIS)

    Salahbiah Abdul Majid; Rusli Ibrahim

    2004-01-01

    Tissue culture technique can be used to obtain complete regeneration of plant cells from shoots, rots, flowers, axillary buds and other parts of the plant. In this study, axillary buds from stem cuttings of Cutting Red, Christine Dior and Mini Rose varieties were used as the stating explants. Murashige and Skoog (1962) media supplemented with 6-Benzylaminopurine (BAP, at 4.44 - 8.88μM/l), Napthaleneacetic acid (NAA at 0.54μM/l),, nad 3% sucrose were used for plantlet initiation and regeneration. Cultured axillary buds were exposed to gamma ray (0.250 Gy/s) at 0, 15, 25, 35, 45, 55, 65 and 75 Gy for radiosensitivity test. From the dose respond curve, LD 5 0 the value for cutting red variety was 25 Gy, Christion Dior 30 Gy and Mini Rose 38 Gy, yet 22% of Mini Rose samples survived at 65 Gy and another 10% at 70 Gy. Screening of M3 plants of irradiated cultured shoots, 2 colour variations were obtained at 40 Gy for Cutting Red variety, while 3 colour variations for Mini Rose at 20 Gy. When 6 varieties of Fragrance Rose were irradiated at 40 Gy, 1 colour variation was obtained from 99 screened plants. This study suggests that the dose range of 20 to 45 can be considered for rose mutagenesis study to produce mutants. (Author)

  14. Influence of pulsing biocides on vase life of cut roses ( Rosa hybrida ...

    African Journals Online (AJOL)

    The results depicted that treatments comprised NaOCl and HQS were able to prolong the vase life of the cut flower stalks up to 15.80 and 15.40 days respectively while Al2(SO4)3 did not offer statistically significant advantage over the use of ordinary tap water. Spectrometric analysis on solution turbidity of the vase solutions ...

  15. Induced mutations in Petunia hybrida Hort

    International Nuclear Information System (INIS)

    Kashikar, S.G.; Khalatkar, A.S.

    1980-01-01

    The seeds of a white flowering strain of Petunia hybrida hort. were treated with different concentrations of ethyl methane-sulphonate, sodium azide, diethyl sulphate, N-methyl-N'-nitro-N-nitrosoguanidine, ethylene imine and gamma radiations. A large number of flower colour and morphological mutants superior to the parental variety were obtained. The flower colour mutations took the form of sectors and whole colour changes. The latter included a large spectrum of colours from light to deep magenta, pink, purple and violet coloured petals. The anthocyanin analysis of these mutants showed different patterns of pigments responsible for the various colours. In addition to these, a broad spectrum of morphological mutations of ornamental value included dwarfs, unbranched, cristata, densa, campyloflora and velutiniflora types. The inheritance of horticulturally important characters was investigated in M 3 and M 4 generations. (author)

  16. Brassinosteroid biosynthesis and signalling in Petunia hybrida.

    Science.gov (United States)

    Verhoef, Nathalie; Yokota, Takao; Shibata, Kyomi; de Boer, Gert-Jan; Gerats, Tom; Vandenbussche, Michiel; Koes, Ronald; Souer, Erik

    2013-05-01

    Brassinosteroids (BRs) are steroidal plant hormones that play an important role in the growth and development of plants. The biosynthesis of sterols and BRs as well as the signalling cascade they induce in plants have been elucidated largely through metabolic studies and the analysis of mutants in Arabidopsis and rice. Only fragmentary details about BR signalling in other plant species are known. Here a forward genetics strategy was used in Petunia hybrida, by which 19 families with phenotypic alterations typical for BR deficiency mutants were identified. In all mutants, the endogenous BR levels were severely reduced. In seven families, the tagged genes were revealed as the petunia BR biosynthesis genes CYP90A1 and CYP85A1 and the BR receptor gene BRI1. In addition, several homologues of key regulators of the BR signalling pathway were cloned from petunia based on homology with their Arabidopsis counterparts, including the BRI1 receptor, a member of the BES1/BZR1 transcription factor family (PhBEH2), and two GSK3-like kinases (PSK8 and PSK9). PhBEH2 was shown to interact with PSK8 and 14-3-3 proteins in yeast, revealing similar interactions to those during BR signalling in Arabidopsis. Interestingly, PhBEH2 also interacted with proteins implicated in other signalling pathways. This suggests that PhBEH2 might function as an important hub in the cross-talk between diverse signalling pathways.

  17. Salvator Rosa e Napoli / Salvator Rosa and Naples

    Directory of Open Access Journals (Sweden)

    Daniela De Liso

    2017-12-01

    Full Text Available Salvator Rosa (Napoli, 1615- Roma 1673 fu pittore e poeta tra i più interessanti del secolo diciassettesimo. Il saggio intende indagare il complesso rapporto artistico e personale che Rosa ebbe con la città di Napoli, in cui era nato e dalla quale era quasi fuggito quando era poco più che un ragazzo. Attraverso una lettura delle principali fonti biobibliografiche a lui contemporanee, di alcune delle sue tele, dei versi delle Satire e di stralci delle lettere che a Napoli dedicherà, s’intende sottolineare che della capitale del Meridione la scrittura e l’arte di Salvator Rosa sono in grado di restituirci un’immagine altra rispetto a quella topica di Napoli gentile, perla luminosa di quella Campania felix che proprio nel Seicento attrae pittori e poeti da ogni parte d’Europa. Nei versi di Rosa, come nella scrittura in prosa, Napoli è, infatti, croce e delizia, prigione da cui fuggire e porto di quell’agognato ritorno che, al di là delle leggendarie asserzioni di alcuni biografi romantici, mai avverrà.   Salvator Rosa (Naples, 1615- Rome, 1673 was a painter and poet among the most interesting of the seventeenth century. The essay intends to investigate the complex artistic and personal relationship that Rosa had with the city of Naples, where she was born and from which she had fled when she was little more than a boy. Through a reading of the main biobibliographic sources to him contemporary, some of his canvases, the verses of the Satire and the excerpts of the letters that Naples will dedicate, it is meant to point out that in the capital of the South the writing and art of Salvator Rosa are Able to give us an image other than the topical one of Napoli's kind, bright pearl of the Campania felix that in the seventeenth century attracts painters and poets from all over Europe. In the lines of Rosa, as in prose writing, Naples is, in fact, a cross and delight, a prison from which to flee and bring about that auspicious return that

  18. Assessing the Pathogenic Ability of Ralstonia pseudosolanacearum (Ralstonia solanacearum Phylotype I from Ornamental Rosa spp. Plants

    Directory of Open Access Journals (Sweden)

    Napoleon N. A. Tjou-Tam-Sin

    2017-11-01

    Full Text Available Ralstonia pseudosolanacearum (Ralstonia solanacearum phylotype I isolates found in stunted, yellowing, and wilted ornamental rose (Rosa spp. were assessed for their pathogenic ability in two rose cultivars (cv. “Armando” and cv. “Red Naomi” and in four solanaceous crops: tomato (Solanum lycopersicum cv. “Money Maker”, tobacco (Nicotiana tabacum cv. “White Burley”, eggplant (Solanum melongena cv. “Black Beauty” and sweet pepper (Capsicum annum cv. “Yolo Wonder”. Significant differences were observed in susceptibility between the two rose cultivars as well as between the two modes of inoculation performed. The cultivar “Armando” was significantly more susceptible than cultivar “Red Naomi,” exhibiting higher disease severity and incidence. Similarly, stem inoculation after wounding was found to be significantly more effective than soil drenching, resulting in higher disease severity. Additionally, a temperature dependency in susceptibility was observed for both cultivars irrespective of the mode of inoculation, however, this was significantly more pronounced upon soil drenching. The solanaceous crops all showed to be susceptible to the R. pseudosolanacearum isolates originated from the Rosa spp. plants. Furthermore, both rose cultivars were able to harbor symptomless infections with other R. pseudosolanacearum and R. solanacearum isolates than those isolated from rose. Our results clearly demonstrated that latent infections in a rose cultivar such as cv. “Red Naomi” do occur even at temperatures as low as 20°C. This latency poses high risks for the entire floricultural industry as latently infected Rosa spp. plants are propagated and distributed over various continents, including areas where climatic conditions are optimal for the pathogen.

  19. Unraveling the genetics of Botrytis cinerea resistance in Gerbera hybrida

    NARCIS (Netherlands)

    Fu, Yiqian

    2017-01-01

    Gerbera hybrida is one of the top five cut flowers. It is well-known to people for its variation in flower color and patterning. Gerbera breeding at the moment is done using conventional methods which are based on a phenotypic selection. This has drawbacks in breeding speed and

  20. Mineral nutrition and in vitro growth of Gerbera hybrida (Asteraceae)

    Science.gov (United States)

    Gerbera hybrida (G. jamesonii x G. viridifolia) or gerbera daisy is one of the most popular flowering ornamental plants worldwide and is sold as bedding, potted plants, and cut flowers. Gerbera daisies are propagated by in vitro culture because conventional propagation methods, by division of clumps...

  1. STUDIES CONCERNING THE INFLUENCE OF BIODEGRADABLE SLOW-RELEASE FERTILIZER USE IN DEVELOPING THE CULTURE OF PETUNIA HYBRIDA SEEDLINGS

    Directory of Open Access Journals (Sweden)

    Mona Popa

    2012-12-01

    Full Text Available Our research on the use of slow-release biodegradable fertilizers were applied to Petunia hybrida seedlings of the variety “White Surfinia”. Thus after 10 days subculturing procedure was to apply fertilizers containing NKP24 biodegradable and made in the form of sticks and granules with six concentrations of starch (5%, 10%, 15%, 20%, 25% and 50% quantaties (4-5 grams/ 1 pot. For each pot with of 8 cm diameter, we prepared a mixture of peat and garden soil in a rate of 1:1. During the vegetation periods, morphological analyses were made regarding the development of Petunia hybrida, cv. “White Surfinia”plants: the length of shoots and number of shoot. Average values recorded from morphological determinations after 1 month of starting experiments on biofertilizers influence on growth and development the seedlings of Petunia was demonstrated that the optimal variant was the fertilizer V5 with -25% WF (wood flour to 50% concentration of biofertilizers NKP24 (for both form of sticks A-big and B-medium and for fertilizer form C- granular the V6 variant with -50% concentration of biofertilizers NKP24, the petunia stem was recorded maximum of 58.92 cm length .The research is part of an international project FP7/2008 with the title "Forest Resource Sustainability through Bio-Based-Composite Development" – FORBIOPLAST. Multiple aims of FORBIOPLAST project are the valorization of forest resources for the production of bio-based products.

  2. ROSA full-core and DNBR capabilities

    International Nuclear Information System (INIS)

    Gibcus, H.P.M.; Verhagen, F.C.M.; Wakker, P.H.

    2013-01-01

    The latest developments of the ROSA (Reloading Optimization by Simulated Annealing) code system with an emphasis on the first full-core version and the minimum DNBR (Departure from Nucleate Boiling Ratio) as a new optimization parameter are presented. Designing the core loading pattern of nuclear power plants is becoming a more and more complex task. This task becomes even more complicated if asymmetries in the core loading pattern arise, for instance due to damaged fuel assemblies. For over almost 2 decades ROSA, NRG's (Nuclear Research and consultancy Group) loading pattern optimization code system for PWRs, has proven to be a valuable tool to reactor operators in accomplishing this task. To improve the use of ROSA for designing asymmetric loading patterns, NRG has developed a full-core version of ROSA besides the original quarter-core version which requires rotational symmetry in the computational domain. The extension of ROSA with DNBR as an optimization parameter is part of ROSA's continuous development. (orig.)

  3. CV equipment responsibilities

    CERN Document Server

    Pirollet, B

    2008-01-01

    This document describes the limits of the responsibilities of the TS/CV for fire fighting equipment at the LHC. The various interfaces, providers and users of the water supply systems and clean water raising systems are described.

  4. Emgu CV essentials

    CERN Document Server

    Shi, Shin

    2013-01-01

    This book provides a practical guide to Emgu CV libraries, with sample code and examples used throughout to explain the concepts clearly. Each chapter deals with a different aspect of the Computer Vision field and the implementation of that topic in Emgu CV.If you are a C# programmer working on computer vision projects, this book is for you. You should have prior experience with C#.

  5. Efficacy of new inhibitors of ethylene perception in improvement of display quality of miniature potted roses (Rosa hybrida L.)

    DEFF Research Database (Denmark)

    Buanong, Mantana; Mibus, Heiko; Sisler, Edward C.

    2005-01-01

    1-Octylcyclopropene (1-OCP)and 1-Decylcyclopropene (1-DCP),ethylene receptor inhibitors,analogues to 1-MCP,substituted with longer carbon chain in the 1-position were investigated in miniature potted roses cultivar ‘Lavender ’.All levels of both chemicals protected as compared to untreated plants.1...

  6. Phosphorus and Nitrogen Regulate Arbuscular Mycorrhizal Symbiosis in Petunia hybrida

    Science.gov (United States)

    Nouri, Eva; Breuillin-Sessoms, Florence; Feller, Urs; Reinhardt, Didier

    2014-01-01

    Phosphorus and nitrogen are essential nutrient elements that are needed by plants in large amounts. The arbuscular mycorrhizal symbiosis between plants and soil fungi improves phosphorus and nitrogen acquisition under limiting conditions. On the other hand, these nutrients influence root colonization by mycorrhizal fungi and symbiotic functioning. This represents a feedback mechanism that allows plants to control the fungal symbiont depending on nutrient requirements and supply. Elevated phosphorus supply has previously been shown to exert strong inhibition of arbuscular mycorrhizal development. Here, we address to what extent inhibition by phosphorus is influenced by other nutritional pathways in the interaction between Petunia hybrida and R. irregularis. We show that phosphorus and nitrogen are the major nutritional determinants of the interaction. Interestingly, the symbiosis-promoting effect of nitrogen starvation dominantly overruled the suppressive effect of high phosphorus nutrition onto arbuscular mycorrhiza, suggesting that plants promote the symbiosis as long as they are limited by one of the two major nutrients. Our results also show that in a given pair of symbiotic partners (Petunia hybrida and R. irregularis), the entire range from mutually symbiotic to parasitic can be observed depending on the nutritional conditions. Taken together, these results reveal complex nutritional feedback mechanisms in the control of root colonization by arbuscular mycorrhizal fungi. PMID:24608923

  7. Phosphorus and nitrogen regulate arbuscular mycorrhizal symbiosis in Petunia hybrida.

    Science.gov (United States)

    Nouri, Eva; Breuillin-Sessoms, Florence; Feller, Urs; Reinhardt, Didier

    2014-01-01

    Phosphorus and nitrogen are essential nutrient elements that are needed by plants in large amounts. The arbuscular mycorrhizal symbiosis between plants and soil fungi improves phosphorus and nitrogen acquisition under limiting conditions. On the other hand, these nutrients influence root colonization by mycorrhizal fungi and symbiotic functioning. This represents a feedback mechanism that allows plants to control the fungal symbiont depending on nutrient requirements and supply. Elevated phosphorus supply has previously been shown to exert strong inhibition of arbuscular mycorrhizal development. Here, we address to what extent inhibition by phosphorus is influenced by other nutritional pathways in the interaction between Petunia hybrida and R. irregularis. We show that phosphorus and nitrogen are the major nutritional determinants of the interaction. Interestingly, the symbiosis-promoting effect of nitrogen starvation dominantly overruled the suppressive effect of high phosphorus nutrition onto arbuscular mycorrhiza, suggesting that plants promote the symbiosis as long as they are limited by one of the two major nutrients. Our results also show that in a given pair of symbiotic partners (Petunia hybrida and R. irregularis), the entire range from mutually symbiotic to parasitic can be observed depending on the nutritional conditions. Taken together, these results reveal complex nutritional feedback mechanisms in the control of root colonization by arbuscular mycorrhizal fungi.

  8. ROSA-II test data report, 6

    International Nuclear Information System (INIS)

    1976-12-01

    Results of the ROSA-II test simulating a loss-of-coolant accident in a light water reactor (LWR) are presented, including the test conditions and interpretation of the phenomena for test runs 411, 314, 315 and 316. (auth.)

  9. (Psidium guajava), hibiscus (Hibiscus-rosa sinensis)

    African Journals Online (AJOL)

    SAM

    2014-04-30

    Hibiscus-rosa ... properly before consumption to avoid ingestion of possible food pathogens that cause food-borne ... depending on the one that most supports their growth ..... boundary leaf, these compounds which include CO2,.

  10. Economic optimization of PWR cores with ROSA

    International Nuclear Information System (INIS)

    Verhagen, F.C.M.; Wakker, P.H.

    2005-01-01

    The core-loading pattern is decisive for fuel cycle economics, fuel safety parameters and economic planning for future cycles. ROSA, NRG's loading pattern optimization code system for PWRs, has proven for over a decade to be a valuable tool to reactor operators for improving their fuel management economics. ROSA uses simulated annealing as loading pattern optimization technique, in combination with an extremely fast 3-D neutronics code for loading pattern calculations. The code is continuously extended with new optimization parameters and rules. This paper outlines recent developments of the ROSA code system and discusses results of PWR specific applications of ROSA. Core designs with a large variety of challenging constraints have been realized with ROSA. As a typical example, for the 193 assembly, Vantage 5H/RFA-2 fueled TVA's Watts Bar unit 1, a cycle 4 core with 76 feed assemblies was designed. This was followed by a high-energy cycle 5 with only 77 feed assemblies and approximately 535 days of natural cycle length. Subsequently, an economical core using 72 bundles was designed for cycle 6. This resulted in considerable savings in the cost of feed assemblies for reloads. The typical accuracy of ROSA compared to results of license codes in within ±0.02 for normalized assembly powers, ±0.03 for maximum enthalpy rise hot channel factor (F ΔH ), and ±3 days for natural cycle length. (author)

  11. ROSA full-core and DNBR capabilities

    International Nuclear Information System (INIS)

    Gibcus, H.P.M.; Verhagen, F.C.M.; Wakker, P.H.

    2012-01-01

    This paper presents the latest developments of the ROSA (Reloading Optimization by Simulated Annealing) code system with an emphasis on the first full-core version and the minimum DNBR (Departure from Nucleate Boiling Ratio) as a new optimization parameter. Designing the core loading pattern of nuclear power plants is becoming a more and more complex task. This task becomes even more complicated if asymmetries in the core loading pattern arise, for instance due to damaged fuel assemblies. For over almost two decades ROSA, NRG's (Nuclear Research and consultancy Group) loading pattern optimization code system for PWRs, has proven to be a valuable tool to reactor operators in accomplishing this task. To improve the use of ROSA for designing asymmetric loading patterns, NRG has developed a full-core version of ROSA besides the original quarter-core version which requires rotational symmetry in the computational domain. The extension of ROSA with DNBR as an optimization parameter is part of ROSA's continuous development. (orig.)

  12. analysis of genetic diversity and relationship of Chinese Rosa rugosa

    African Journals Online (AJOL)

    Administrator

    2011-05-30

    May 30, 2011 ... Rosa rugosa is a deciduous shrub of the genus Rosa, having prickly stems ... eastern Shandong province and Tumen River estuary ..... Smithsonian Institution, Washington DC, USA. Sokolov SY ... Distribution of trees and.

  13. CV : [luuletused] / Mari Vallisoo

    Index Scriptorium Estoniae

    Vallisoo, Mari, 1950-2013

    2007-01-01

    Sisu: CV ; Sõnaraamat ; Tegijad ; "Kus leemekulp?..." ; "Veel üks viimnepäev mis asja teha sellega..." ; "Ülemise riiuli ääre pääl...", "Alkeemik Alberti elamus...", "Valge pulber veel enam valgega...", "Meie isa, kes sa...", "Ükskord, kui Maa...", "Kevadvesi ja -pori...", "Mõisnik Meeskurat müüs mu vaarisale..."

  14. Prediction of ROSA-III experiment

    International Nuclear Information System (INIS)

    Soda, Kunihisa

    1978-06-01

    ROSA-III experiment with the simulated BWR system is to investigate thermal hydraulic behavior as well as ECCS performance in a postulated loss-of-coolant accident. RUN 701 assumes average core power, high and low pressure core sprays and low pressure injection of ECCS. Prediction of experiment RUN 701 was made with computer code RELAP-4J. The results indicate the need for ROSA-III pump characteristics to be clarified and for liquid level formation model to be improved. Comparison of the prediction results with the experimental data should reveal the areas of modifications in calculation model. (auth.)

  15. Salvator Rosa's Democritus and Diogenes in Copenhagen

    DEFF Research Database (Denmark)

    Pedersen, Eva de la Fuente

    2017-01-01

    the aesthetic and the ethical in the artist and the work of art, to the melancholic temperament and to the concept of genius as is appeared within the art theoretical context of the day. The article shows that the two philosopher-paintings may have been included amongst what Salvator Rosa himself regarded...

  16. Biologische beheersing wortelvlieg (Psila rosae) in 2009

    NARCIS (Netherlands)

    Broek, van den R.C.F.M.; Gruppen, R.; Kamstra, J.H.

    2010-01-01

    Since 2007 Applied Plant Research (PPO) investigates whether the carrot root fly (Psila rosae) can be disorientated by applying strong smelling oils. A mature carrot root fly can find a carrot field by smell. The question is whether the smell of carrots may be masked by strong smelling oils so that

  17. ( Rosa damascena Mill.) by microbial inoculation

    African Journals Online (AJOL)

    This study was carried out to determine the effects of microbial inoculation in breaking seed dormancy and on the germination of Rosa damascena Mill. Seeds of R. damascena Mill. are the most used scented rose species in rose oil production. The most important production centers around the world are Turkey and ...

  18. RETHINKING ROMANZO ROSA: THE FIRST PERSON NARRATOR ...

    African Journals Online (AJOL)

    User

    marketing strategy elaborated during the interwar years and now in full ... For a comprehensive analysis of romanzo rosa, see also Pozzato (1982) and ... position of capturing the changing social climate and women's search for ... its name into Annabella in 1938, as a consequence of the campaign against ...... Milan: Net.

  19. Estado nutrimental del agroecosistema rosa (Rosa spp. en la ladera este del Iztaccíhuatl

    Directory of Open Access Journals (Sweden)

    Steinger Cortés Jiménez

    2017-07-01

    Full Text Available Aproximadamente 3000 ha se encuentran plantadas con rosas (Rosa spp. en el estado de Puebla. El valor estimado generado por esta actividad es 625 millones de pesos y permite incorporar a la mujer a la economía regional. El uso de fertilización nitrogenada en rosa de corte, mayor a 7000 kg ha-1, es excesiva y afecta la salud del suelo y la economía de los productores. Los objetivos del trabajo fueron: (1 evaluar el estado actual de la fertilidad de los suelos en la zona de producción de rosa del DDR 05 San Martín Texmelucan y (2 estudiar el efecto del aporte de tres fuentes de materia orgánica en la producción, calidad y estado nutrimental de tres variedades de rosas, así como en la condición física de los suelos. Para ello se evaluaron algunas propiedades químicas en muestras de suelo de 112 invernaderos localizados en cuatro unidades edafoclimáticas. Se usaron tres tipos de materia orgánica (MO: compost comercial, estiércol de bovino y gallinaza y tres variedades de rosa: Anastasia, Selena y Latin. El diseño fue completamente al azar modificado y se realizaron análisis de varianza y comparaciones de medias. Los suelos presentaron condiciones de fertilidad contrastantes. La gallinaza aumentó el rendimiento en la variedad Anastasia y el compost en la variedad Selena. La calidad de las tres variedades de rosas aumentó con el aporte de las tres fuentes de MO a los tres meses, pero disminuyó a los nueve meses. Las tres fuentes de MO aportaron niveles similares de macroelementos a la planta y mejoraron la condición física del suelo al aumentar el porcentaje de macroagregados en todos los tratamientos. El mayor porcentaje de macroagregados se obtuvo por la adición de compost en los suelos con las variedades Anastasia y Selena. Los suelos de los invernaderos del agrosistema rosa (Rosa spp. presentaron características químicas y condiciones de fertilidad contrastantes, producto de una variabilidad edafoclimática. Las fuentes de

  20. Spatial neutron kinetic module of ROSA code

    International Nuclear Information System (INIS)

    Cherezov, A.L.; Shchukin, N.V.

    2009-01-01

    A spatial neutron kinetic module was developed for computer code ROSA. The paper describes a numerical scheme used in the module for resolving neutron kinetic equations. Analytical integration for delayed neutrons emitters method and direct numerical integration method (Gear's method) were analyzed. The two methods were compared on their efficiency and accuracy. Both methods were verified with test problems. The results obtained in the verification studies were presented [ru

  1. Mutagenesis of hibiscus rosa-sinensis

    International Nuclear Information System (INIS)

    Shuhaimi Shamsudin; Shakinah Salleh; Mohd Nazir Basiran

    2005-01-01

    Mutation induction is an alternative method to create more variation in Hibiscus rosa-sinensis. Radiosensitivity test was carried out to determine the effective doses for irradiation of stem cuttings. Stem cuttings of Hibiscus rosa-sinensis were irradiated at 10, 20, 30 and 60 Gy using a gamma cell with a Co-60 source at a dose rate of 1.66 Gys -1 . Irradiated stem cuttings were planted in sand-beds and data on the number of growing shoots were taken. Increasing gamma ray doses resulted in a reduction of growing shoots on the irradiated stem cuttings. It was shown that the LD50 for the stem cuttings was 36.2 Gy and at 75% growth was 17.15 Gy. Based on these results, 20 to 30 Gy were chosen for irradiation of the stem cuttings to induce mutation in Hibiscus rosa-sinensis. Irradiated stem cuttings were then planted into sand-beds for rooting. After one month, the rooted stems were transferred into polybags and allowed to grow under 70% shade provided by plastic netting and the variants were subsequently observed. Three variants with variation in flower shapes and colours were obtained. (Author)

  2. Cytoplasmic male sterility in Petunia hybrida : a structural and histochemical analysis

    NARCIS (Netherlands)

    Bino, R.J.

    1986-01-01

    This thesis presents an analysis of the structural and histochemical aspects of cytoplasmic male sterility (cms) in Petuniahybrida . In petunia and in other crops, cms is the most commonly used tool for hybrid seed production. Application of the trait

  3. NEC1, a regulator of nectary development and nectar production in Petunia hybrida

    NARCIS (Netherlands)

    Ge, Ya-Xin

    2001-01-01

    This thesis describes an efficient cloning, characterization and functional analysis of a nectary-specific gene in Petunia hybrida. NEC1, a novel gene, is highly expressed in nectaries. NEC1 is involved in nectar production and nectar secretion. Nectar secreted from floral nectaries is the main

  4. Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida

    NARCIS (Netherlands)

    Bombarely, Aureliano; Moser, Michel; Amrad, Avichai; Bao, Manzhu; Bapaume, Laure; Barry, Cornelius S.; Bliek, Mattijs; Boersma, Maaike R.; Borghi, Lorenzo; Bruggmann, Rémy; Bucher, Marcel; Agostino, D' Nunzio; Davies, Kevin; Druege, Uwe; Dudareva, Natalia; Egea-Cortines, Marcos; Delledonne, Massimo; Fernandez-Pozo, Noe; Franken, Philipp; Grandont, Laurie; Heslop-Harrison, J.S.; Hintzsche, Jennifer; Johns, Mitrick; Koes, Ronald; Lv, Xiaodan; Lyons, Eric; Malla, Diwa; Martinoia, Enrico; Mattson, Neil S.; Morel, Patrice; Mueller, Lukas A.; Muhlemann, Joëlle; Nouri, Eva; Passeri, Valentina; Pezzotti, Mario; Qi, Qinzhou; Reinhardt, Didier; Rich, Melanie; Richert-Pöggeler, Katja R.; Robbins, Tim P.; Schatz, Michael C.; Schranz, Eric; Schuurink, Robert C.; Schwarzacher, Trude; Spelt, Kees; Tang, Haibao; Urbanus, Susan L.; Vandenbussche, Michiel; Vijverberg, Kitty; Villarino, Gonzalo H.; Warner, Ryan M.; Weiss, Julia; Yue, Zhen; Zethof, Jan; Quattrocchio, Francesca; Sims, Thomas L.; Kuhlemeier, Cris

    2016-01-01

    Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid

  5. The ultrastructure of chloroplasts in variegata irregulare mutants of garden petunias (Petunia hybrida hort. superbissima

    Directory of Open Access Journals (Sweden)

    Stanisław Muszyński

    2015-01-01

    Full Text Available The ultrastructure of mutated chloroplasts in tetraploid garden petunias (Petunia hybrida hort. superbissima was analyzed by electron microscopy. The formation of grana structure is inhibited after secondary thylacoids start forming. Rapid dezintegration of the structure is observed. It is suggested that a substance responsible for photostabilization of grana structure is lacking.

  6. Characterization of a Nepovirus causing a leaf mottling disease in Petunia hybrida

    Science.gov (United States)

    This report describes the complete genome sequence and characterization of a new virus infecting petunia. Icosahedral virus-like particles were isolated from Petunia hybrida cuttings with interveinal chlorotic mottling. The virus was transmitted by mechanical inoculation from infected to healthy P. ...

  7. Evidence for DNA repair after ultraviolet irradiation of Petunia hybrida pollen

    International Nuclear Information System (INIS)

    Jackson, J.F.; Linskens, H.F.; Katholieke Universiteit Nijmegen; Katholike Universiteit Nijmegen

    1978-01-01

    Ultraviolet irradiation of Petunia hybrida pollen led to an unscheduled labelling of pollen DNA by 3 H-thymidine during the early stages of germination. Hydroxyurea increased this DNA labelling, while added boron, required absolutely for pollen germination, tube elongation and tube generative cell mitosis, was not needed for this repair-like DNA synthesis. (orig.) [de

  8. Estado Actual de Peronospora sparsa, Causante del Mildiu Velloso en Rosa ( Rosa sp.)

    OpenAIRE

    Pablo Israel Álvarez Romero

    2013-01-01

    La rosa ( Rosa sp.) es una ornamental de importancia en México. Es afectada por un complejo de enfermedades, destacándose el Mildiu velloso ocasionado por el Chromista Per onospora sparsa que incide en la producti vidad, calidad, comercia lización y costos de producción, las pérdidas llegan hasta el 100 % de los ta llos florales. Entre los fungicidas empleados para el control del Mildiu velloso se destacan: fosetil-Al, dimetomorph, c y m o x a n i l , m e t a l a x y l . A l m o m e n t o e x...

  9. Potencial uso de la leonardita para el cultivo de rosa en condiciones de invernadero

    Directory of Open Access Journals (Sweden)

    Marilú González

    2018-01-01

    Full Text Available La leonardita es un fertilizante orgánico que está teniendo cada vez mayor aceptación entre los agricultores, debido a que contiene altas cantidades de ácidos húmicos que favorecen la producción de los cultivos. Se evaluó el efecto de la aplicación de leonardita sobre parámetros vegetativos y productivos de rosa (Rosa sinensis cv. Freedom bajo condiciones de invernadero. Se evaluaron 3 dosis del ácidos orgánicos derivados del mineral leonardita: baja (10 g.cama-1, media (15 g.cama-1 y alta (20 g.cama-1 lo cual fue comparado con el efecto de un ácido orgánico comúnmente usado en el invernadero (control positivo y con la fertilización inorgánica (control negativo. Las aplicaciones fueron realizadas al inicio del ensayo sobre un área de 18 m2 por tratamiento y las plantas fueron evaluadas semanalmente durante 5 semanas. Los resultados obtenidos mostraron que las plantas tratadas con 15 g de leonardita.cama-1 presentaron mayor longitud de brotes, número de brotes basales y productividad de tallos en comparación con el resto de los tratamientos. Sin embargo, se sugiere realizar estudios similares donde se evalúe durante un periodo más prolongado para determinar el tiempo que se mantiene el efecto sobre la productividad en el cultivo de rosas en condiciones de invernadero.

  10. The Myth of "Rosa Parks the Tired." Teaching about Rosa Parks and the Montgomery Bus Boycott.

    Science.gov (United States)

    Kohl, Herbert

    1993-01-01

    Retells the story of Rosa Parks and the Montgomery (Alabama) bus boycott to reflect more accurately the cultural and historical background of the boycott and the conscious decision made by Mrs. Parks. Accurate examination of the story actually enhances a child's ability to identify with the issues and the protagonists. (SLD)

  11. La España de Rosas Blancas = The Spain of Rosas Blancas

    Directory of Open Access Journals (Sweden)

    Óscar Gual Boronat

    2015-06-01

    Full Text Available Mediante la lectura del cuaderno femenino español Rosas blancas, publicado por la editorial Toray entre 1958 y 1965, tratamos de estudiar la capacidad del cómic para reflejar el papel de la mujer durante una de las etapas clave de la dictadura franquista. Tras trazar un recorrido por los tebeos sentimentales de la década de 1940 (Azucena y las primeras revistas modernas para niñas (Florita y Sissi, analizaremos el contenido de dicha publicación. En ese análisis percibiremos cómo Rosas blancas prolongará determinados mitos de aquellas historietas al tiempo que introducirá nuevas perspectivas en consonancia con lo que estaba sucediendo en la sociedad española.Through the reading of the Spanish female comic-book Rosas blancas, published by Editorial Toray between 1958 and 1965, we try to explore the ability of comics to reflect the role of women during the key stages of the Franco dictatorship. After a study of the sentimental comics of the 1940s (Azucena and the first modern magazines for girls (Florita and Sissi, we analyse the content of this publication. In this analysis we perceive how Rosas blancas extend certain myths while those cartoons introduce new perspectives in line with what was happening in the Spanish society.

  12. Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in Gerbera hybrida

    Directory of Open Access Journals (Sweden)

    Gan Huang

    2017-05-01

    Full Text Available Gerbera hybrida is a cut-flower crop of global importance, and an understanding of the mechanisms underlying petal development is vital for the continued commercial development of this plant species. Brassinosteroids (BRs, a class of phytohormones, are known to play a major role in cell expansion, but their effect on petal growth in G. hybrida is largely unexplored. In this study, we found that the brassinolide (BL, the most active BR, promotes petal growth by lengthening cells in the middle and basal regions of petals, and that this effect on petal growth was greater than that of gibberellin (GA. The RNA-seq (high-throughput cDNA sequencing technique was employed to investigate the regulatory mechanisms by which BRs control petal growth. A global transcriptome analysis of the response to BRs in petals was conducted and target genes regulated by BR were identified. These differentially expressed genes (DEGs include various transcription factors (TFs that were activated during the early stage (0.5 h of BL treatment, as well as cell wall proteins whose expression was regulated at a late stage (10 h. BR-responsive DEGs are involved in multiple plant hormone signal pathways, hormone biosynthesis and biotic and abiotic stress responses, showing that the regulation of petal growth by BRs is a complex network of processes. Thus, our study provides new insights at the transcriptional level into the molecular mechanisms of BR regulation of petal growth in G. hybrida.

  13. INFLUENCE OF THE NATURAL ILLUMINATION LEVEL ON THE INDOOR GROWTH AND DEVELOPMENT OF PETUNIA HYBRIDA

    Directory of Open Access Journals (Sweden)

    B. Lixandru

    2004-01-01

    Full Text Available In a room with a natural illumination index (NII of 4.79, for 70 days, the influence of three illumination levels on the growth and development process of petunias from the Petunia hybrida species was studied. After rising, plants were placed in three zones of the room with different illumination levels: zone A (683 lx – situated on the window’s sill, zone B (113 lx – situated on the floor, at the base of the parapet, and zone C (376 lx – situated in the center of the room, on a table, at 80 cm from the floor. After two weeks from the pricking out, plants were transplanted in glass pots of 200 ml. At 2, 15, 30, 50 and 70 days the maximum length of the stem, branching level and leaf number were determined. At the end of the experiment and 10 days of room temperature drying, root, stem, leaf and flower biomass quantity was determined. Our results evidence the negative effect of the low illumination level (113 lx and 376 lx on the growth and development process of petunias from the Petunia hybrida species. Being a light and warmth loving plant, P. hybrida may be grown only at well-illuminated windows, with a level of minimum 1000 lx.

  14. ANTIMICROBIAL ACTIVITY OF ROSA CANINA FLOWERS AGAINST SELECTED MICROORGANISMS

    Directory of Open Access Journals (Sweden)

    Katarína Rovná

    2015-02-01

    Full Text Available Rosa canina flowers were screened against various plant pathogenic microbial strains to study the antimicrobial properties of the plant. Ethanolic and methanolic extracts of flowers were screened applying agar well diffusion method against two Gram-negative bacteria including Escherichia coli CCM 3988 and Pseudomonas aeruginosa CCM 1960 and three microscopic filamentous fungi strains Aspergillus niger, Fusarium culmorum and Alternaria alternata, respectively. The best antimicrobial effect of ethanolic extract of Rosa canina flowers was found against Pseudomonas aeruginosa and the best antimicrobial effect of methanolic extract of Rosa canina flowers was found against Escherichia coli.

  15. PhMYB4 fine-tunes the floral volatile signature of Petunia x hybrida through PhC4H.

    Science.gov (United States)

    Colquhoun, Thomas A; Kim, Joo Young; Wedde, Ashlyn E; Levin, Laura A; Schmitt, Kyle C; Schuurink, Robert C; Clark, David G

    2011-01-01

    In Petunia × hybrida cv 'Mitchell Diploid' (MD), floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is controlled spatially, developmentally, and daily at molecular, metabolic, and biochemical levels. Multiple genes have been shown to encode proteins that either directly catalyse a biochemical reaction yielding FVBP compounds or are involved in metabolite flux prior to the formation of FVBP compounds. It was hypothesized that multiple transcription factors are involved in the precise regulation of all necessary genes, resulting in the specific volatile signature of MD flowers. After acquiring all available petunia transcript sequences with homology to Arabidopsis thaliana R2R3-MYB transcription factors, PhMYB4 (named for its close identity to AtMYB4) was identified, cloned, and characterized. PhMYB4 transcripts accumulate to relatively high levels in floral tissues at anthesis and throughout open flower stages, which coincides with the spatial and developmental distribution of FVBP production and emission. Upon RNAi suppression of PhMYB4 (ir-PhMYB4) both petunia cinnamate-4-hydroxylase (PhC4H1 and PhC4H2) gene transcript levels were significantly increased. In addition, ir-PhMYB4 plants emit higher levels of FVBP compounds derived from p-coumaric acid (isoeugenol and eugenol) compared with MD. Together, these results indicate that PhMYB4 functions in the repression of C4H transcription, indirectly controlling the balance of FVBP production in petunia floral tissue (i.e. fine-tunes).

  16. Instant OpenCV starter

    CERN Document Server

    Dalal, Jayneil

    2013-01-01

    Get to grips with a new technology, understand what it is and what it can do for you, and then get to work with the most important features and tasks.A practical, quick, and hands-on guide for Python developers and hobbyists who want to get started with computer vision with OpenCV.This book is great for developers, hobbyists, and students new to computer vision who are looking to get a good grounding in how to use the OpenCV library. It's assumed that you will have some basic experience in C/C++ programming.

  17. ROSA-II test data report, 10

    International Nuclear Information System (INIS)

    1977-12-01

    Results of the ROSA-II test simulating a loss-of coolant accident (LOCA) in a light water reactor (LWR) are presented, including the test conditions and interpretation of the phenomena for test runs 415, 417, 421 and 422. Even in small break at the cold leg, the core is exposed to void and the temperature rises. In small break of the hot leg, however, core cooling keeps without temperature rise, because there still remains much residual water and upward core flow exists. Direct effect of the HPCI on the depressurization rate is small, but it increases the accumulator injection rate, leading to early core reflooding and early core cooling from upward. Effects of the secondary system depressurization are increase of depressurization and discharge rates of the primary loop, which results in early initiation of the accumulator injection and core reflooding. (auth.)

  18. Solar drying of rose (Rosa sp.) petals

    Energy Technology Data Exchange (ETDEWEB)

    Balladin, D.A.; Headley, Oliver [University of the West Indies, Center for Resource Management and Environmental Studies, St. Michael (Barbados)

    1999-10-01

    The rose (Rosa sp.) petals can be dried after 2 days at about 30degC reaching an equilibrium moisture content after 16 h using the solar wire basket dryer. The initial moisture content (wet wt basis) and final moisture content (dry wt basis), determined by the Dean-Stark toluene were 65.7 and 25.2% respectively. The intensity of the rose red coloured pigment (pelargonidin) decreased by a factor of 2.5 after drying. The pelargonidin ethanoic extract as an acid-base indicator, has a K{sub 4} value of 1 x 10{sup -4} mol 1{sup -1} and pH of end point 4 and imbibed on filter paper and allowed to air dry for 5 min showed excellent properties as acid-based test tapes. (Author)

  19. ROSA-II test data report, 13

    International Nuclear Information System (INIS)

    1978-07-01

    Results of the ROSA-II test simulating a loss-of-coolant accident (LOCA) in a PWR are presented, including test conditions and interpretations of phenomena observed in test runs 502, 505, 506 and 507. Development tests were performed to find a more effective ECCS injection method than the existing one based on cold leg injection. A combined injection of hot water into upper plenum in early stage of blowdown and subsequent cold water into lower plenum is the most effective method for a cold leg break. A hot leg injection of a low pressure injection system is effective for direct core cooling and early reflooding. The generalization for actual reactors will require analyses with a reliable code. (auth.)

  20. Identification of antimutagenic properties of anthocyanins and other polyphenols from rose (Rosa centifolia) petals and tea.

    Science.gov (United States)

    Kumar, Sanjeev; Gautam, Satyendra; Sharma, Arun

    2013-06-01

    Petals from different rose (Rosa centifolia) cultivars ("passion," "pink noblesse," and "sphinx") were assessed for antimutagenicity using Escherichia coli RNA polymerase B (rpoB)-based Rif (S) →Rif (R) (rifampicin sensitive to resistant) forward mutation assay against ethyl methanesulfonate (EMS)-induced mutagenesis. The aqueous extracts of rose petals from different cultivars exhibited a wide variation in their antimutagenicity. Among these, cv. "passion" was found to display maximum antimutagenicity. Upon further fractionation, the anthocyanin extract of cv. "passion" displayed significantly higher antimutagenicity than its phenolic extract. During thin-layer chromatography (TLC) analysis, the anthocyanin extract got resolved into 3 spots: yellow (Rf : 0.14), blue (Rf : 0.30), and pink (Rf : 0.49). Among these spots, the blue one displayed significantly higher antimutagenicity than the other 2. Upon high-performance liquid chromatography analysis, this blue spot further got resolved into 2 peaks (Rt : 2.7 and 3.8 min). The 2nd peak (Rt : 3.8 min) displaying high antimutagenicity was identified by ESI-IT-MS/MS analysis as peonidin 3-glucoside, whereas less antimutagenic peak 1 (Rt : 2.7) was identified as cyanidin 3, 5-diglucoside. The other TLC bands were also characterized by ESI-IT-MS/MS analysis. The least antimutagenic pink band (Rf : 0.49) was identified as malvidin 3-acetylglucoside-4-vinylcatechol, whereas non-antimutagenic yellow band (Rf : 0.14) was identified as luteolinidin anthocyanin derivative. Interestingly, the anthocyanin extracted from rose tea of cv. "passion" exhibited a similar antimutagenicity as that of the raw rose petal indicating the thermal stability of the contributing bioactive(s). The findings thus indicated the health protective property of differently colored rose cultivars and the nature of their active bioingredients. © 2013 Institute of Food Technologists®

  1. ROSA-II test data report, 11

    International Nuclear Information System (INIS)

    1978-02-01

    Results of the ROSA-II tests simulating a loss-of-coolant accident (LOCA) and effects of an emergency core cooling system (ECCS) in a pressurized water reactor (PWR) are presented including the test conditions and interpretations of the data in test runs 327,328,329 and 330. Each test was performed with large double-ended hot leg break and effect of the break area distribution (break diameter are 25.0 mm at one end and 37.5 mm at the other end of break) and of pump circulation upon coolant flow in the core were studied. The following are the results: In the case of a smaller break on the steam generator side, core cooling was achieved due to upward coolant flow in the core and early reflooding by ACC water injected into the cold leg. In the case of a smaller break area on the vessel side, on the other hand, coolant flow in the core was stagnant and the heater rods were mostly exposed to steam, so that core cooling was not as good. Effect of the coolant circulation by acting pump on the core cooling during a blowdown was not significant except that in a steam generator side small break the core cooling was improved. (auth.)

  2. Localization of membrane-associated calcium in unpollinated and pollinated pistil of Petunia hybrida Hort.

    Directory of Open Access Journals (Sweden)

    Elżbieta Bednarska

    2014-01-01

    Full Text Available In the pistil of Petunia hybrida, the transmitting tract and the ovules are the sites which give Ca2+-CTC fluorescence. In unpollinated pistil the level of membrane-associated Ca2+ decreases from the stigma to the base of the style. The renewed strong rise of Ca2+-CTC fluorescence appears on the placenta surface and in the ovule integuments. Following pollination, when the pollen tubes have grown through the pistil, the pattern of membrane-associated Ca2+ on the path stigma - ovary is reversed. The highest fluorescence is found in the base of the style. In pollinated ovules the Ca2+-CTC fluorescence increases markedly. In the transmitting cells membrane-associated Ca2+ occurs mainly in the polar regions of the cell. During cell degeneration following pollination the cytoplasmic clusters show Ca2+-CTC fluorescence. The used P. hybrida cultivar is self-fertile. The selection of pollen tubes occurs mainly in the upper part of the style. The rejected pollen tubes show a steady high level of membrane-associated calcium.

  3. Setenta anos depois: direitos autorais em Noel Rosa Seventy years later: Noel Rosa's copyrights

    Directory of Open Access Journals (Sweden)

    Daniel do Amaral Arbix

    2008-06-01

    Full Text Available Um regime ótimo de direitos autorais, ao tomar fundamento no estímulo ao desenvolvimento de novos bens intelectuais, depende de limites bem traçados. Este artigo procura aferir a funcionalidade desses direitos no que diz respeito a composições musicais, de sorte a avaliar, nesse campo específico, a maleabilidade da proteção aos direitos autorais e sua adequação à criação de novas obras. Para tanto, remete-se à experiência criativa de e sobre noel rosa, expoente do samba brasileiro. A marcada assunção de riscos por autores de obras derivadas, a formalização irregular das relações jurídicas entre titulares de direitos autorais e a profissionalização de determinados autores são constatadas como respostas diretas aos incentivos legais pertinentes. A incerteza decorrente dessas respostas expõe a dessintonia entre alguns dos objetivos das normas analisadas e seus efeitos práticos.An optimal copyright regime, based on fostering the development of new intellectual works, depends on well designed limitations. This article aims to analyze such rights' functionality with respect to musical works, in order to evaluate, in this field specifically, the flexibility of copyright protection and its adequacy for the creation of new works. To perform such task, the creative process of brazilian samba expert, noel rosa, is examined. A remarkable assumption of risks by derivative works' authors, the irregular formalization of legal relations between copyright owners and the profissionalization of certain authors are seen as direct answers to the legal incentives currently in force. The uncertainty derived from such answers exposes the tension between some of the analyzed norms' objectives and their actual effects.

  4. Studies on the constituents of hibiscus rosa-sinensis

    International Nuclear Information System (INIS)

    Hossain, M.A.; Tarafdar, S.A.

    2003-01-01

    A new flavone derivative, furano flavone (1) was isolated along with ovalichalcone (2) from the leaves and stems of Hibiscus rosa-sinensis (Malvaceae). The structure of furanoflavone (1) was identified as 5-methoxy-3' methyl-3', 4'-methylenedioxyfurano(2,3; 7,8) flavone by spectroscopic and chemical analysis. (author)

  5. Membrane Stabilizing Activity And Phytochemistry Of Hibiscus rosa ...

    African Journals Online (AJOL)

    The human erythrocyte membrane stabilizing activity of saline extract of Hibiscus rosa-sinensis leaves was investigated as part of efforts at validating its use as anti-arthritic and anti-inflammatory agent. The results of the membrane stabilizing activity of the extract, when compared to two non-steroidal anti-inflammatory drugs ...

  6. Profile of the Phenolic Compounds of Rosa rugosa Petals

    Directory of Open Access Journals (Sweden)

    Andrzej Cendrowski

    2017-01-01

    Full Text Available Rosa rugosa petals are a rich source of phenolic compounds, which determined their antioxidant properties. The aim of this study was to determine the polyphenolic composition of not processed petals of Rosa rugosa collected from the commodity crops and to determine the variability of the contained therein polyphenols between harvesting seasons. Twenty polyphenols were identified by UPLC-ESI-MS. The main fraction of polyphenols was ellagitannins, which are 69 to 74% of the total polyphenols of the petals. In the petals of Rosa rugosa, four anthocyanins have been identified: cyanidin 3,5-di-O-glucoside, peonidin 3-O-sophoroside, peonidin 3,5-di-O-glucoside, and peonidin 3-O-glucoside, of which the predominant peonidin 3,5-di-O-glucoside represented approx. 85% of all the determined anthocyanin compounds. It was found that the petals of Rosa rugosa are a valuable source of bioactive compounds and can be considered as a healthy valuable resource.

  7. Genetic relationships among Rosa species based on random ...

    African Journals Online (AJOL)

    To investigate the genetic diversity of Rosa accessions, random amplified polymorphism DNA (RAPD) approach was employed. Nine of ten primers amplified 138 scorable RAPD loci with 111 polymorphic bands (80%). Percentages of polymorphic bands ranged from 75 to 100%. Sizes of amplified DNA fragments ranged ...

  8. Miki ja Rosa hõivasid Tartu / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-2016

    2001-01-01

    Lühidalt viiest kunstinäitusest - Vaikust lõhestas vali kärgatus (Peeter Allik), Küla, mida on põletanud punased õhtud (Imat Suumann), Rosa Permanente (Ruth Huimerind), Miki-Hiire kirik (Erki Meister, Christer Hägglund), Aledoia (Andrus Joonas)

  9. Effect of Plant Growth Regulators and Cold on Improvement of Morphological Characteristics of Cineraria (Pericallis × hybrida

    Directory of Open Access Journals (Sweden)

    Kazem Bashiri

    2017-12-01

    Full Text Available Introduction: The climate of every region does not let to have year-round production of crops. Use of plant growth regulators allows to produce the flowers out of season and improve their quality and yield. Gibberellin is one of the plant growth regulators which can substitute cold requirement of plants, while cytokinin is another plant growth regulator to stimulate floral initials. Paclobutrazol is a triazole compound that inhibits gibberellin synthesis. Cineraria (Pericallis × hybrida as a prominent flowering pot plant has a growing demand during the spring festivals especially Nowrouz, which needs further improvement of quality and yield. In this regard a study was designed to examine the morphological characteristics of cineraria using gibberellin, cytokinin, paclobutrazol and cold. Materials and Methods: Seeds of cineraria (Pericallis × hybrida cv. Satellite( were sown in plug trays at the research greenhouse of college of agriculture, Shiraz University. A study was carried out with a completely randomized design and five replications. Control plants were transferred to incubators at eight leaves stage to receive six weeks of cold. Spraying treatments consisted of control (water, 100 mg/L gibberellin at three times (before cold, middle of cold and after cold, combinations of cold and/or gibberellin (100, 200 mg/L with benzyl adenine (150, 300 mg/L and/or paclobutrazol (250, 500 mg/L. In order to better understand the effects of paclobutrazol, its treatments were applied two weeks after gibberellin treatments. Data analysis was done by SAS 9.1 software and means were compared by LSD at 5 percent probability level. Results and Discussions: Gibberellin can be used as a replacement for cold. The maximum acceleration of full flowering (32 days and the greatest delay of full bloom (14 days were observed in 100 mg/L gibberellin + 250 mg/L paclobutrazol and 200 mg/L gibberellin + 300 mg/L benzyl adenine, respectively. These results were

  10. Characterization of PhPRP1, a histidine domain arabinogalactan protein from Petunia hybrida pistils.

    Science.gov (United States)

    Twomey, Megan C; Brooks, Jenna K; Corey, Jillaine M; Singh-Cundy, Anu

    2013-10-15

    An arabinogalactan protein, PhPRP1, was purified from Petunia hybrida pistils and shown to be orthologous to TTS-1 and TTS-2 from Nicotiana tabacum and NaTTS from Nicotiana alata. Sequence comparisons among these proteins, and CaPRP1 from Capsicum annuum, reveal a conserved histidine-rich domain and two hypervariable domains. Immunoblots show that TTS-1 and PhPRP1 are also expressed in vegetative tissues of tobacco and petunia respectively. In contrast to the molecular mass heterogeneity displayed by the pistil proteins, the different isoforms found in seedlings, roots, and leaves each has a discrete size (37, 80, 160, and 200 kDa) on SDS-PAGE gels. On the basis of their chemistry, distinctive domain architecture, and the unique pattern of expression, we have named this group of proteins HD-AGPs (histidine domain-arabinogalactan proteins). Copyright © 2013 Elsevier GmbH. All rights reserved.

  11. Antifungal activity of saponins originated from Medicago hybrida against some ornamental plant pathogens

    Directory of Open Access Journals (Sweden)

    Alicja Saniewska

    2012-12-01

    Full Text Available Antifungal activity of total saponins originated from roots of Medicago hybrida (Pourret Trautv. were evaluated in vitro against six pathogenic fungi and eight individual major saponin glycosides were tested against one of the most susceptible fungi. The total saponins showed fungitoxic effect at all investigated concentrations (0.01%, 0.05% and 0.1% but their potency was different for individual fungi. The highest saponin concentration (0.1% was the most effective and the inhibition of Fusarium oxysporum f. sp. callistephi, Botrytis cinerea, Botrytis tulipae, Phoma narcissi, Fusarium oxysporum f. sp. narcissi was 84.4%, 69.9%, 68.6%, 57.2%, 55.0%, respectively. While Fusarium oxysporum Schlecht., a pathogen of Muscari armeniacum, was inhibited by 9.5% only. Eight major saponin glycosides isolated from the total saponins of M. hybrida roots were tested against the mycelium growth of Botrytis tulipae. The mycelium growth of the pathogen was greatly inhibited by hederagenin 3-O-β-D-glucopyranoside and medicagenic acid 3-O-β-D-glucopyranoside. Medicagenic acid 3-O-β-D-glucuronopyranosyl-28-O-β-D-glucopyranoside and oleanolic acid 3-O-[β-D-glucuronopyranosyl(1→2-α-L-galactopyranosyl]-28-O-β-D-glucopyranoside showed low fungitoxic activity. Medicagenic acid 3-O-a-D-glucopyranosyl- 28-O-β-D-glucopyranoside, hederagenin 3-O-[α-L- hamnopyranosyl(1→2-β-D-glucopyranosyl(1→2-β-D-glucopyranosyl]- 28-O-α-D-glucopyranoside and hederagenin 3-O-β-D-glucuronopyranosyl-28-O-β-D- lucopyranoside did not limit or only slightly inhibited growth of the tested pathogen. While 2β, 3β-dihydroxyolean-12 ene-23-al-28-oic acid 3-O-β-D-glucuronopyranosyl-28-O-β-D-glucopyranoside slightly stimulated mycelium growth of B. tulipae.

  12. A morphological and cytological study of Petunia hybrida exposed to UV-B radiation

    International Nuclear Information System (INIS)

    Staxén, I.; Bornmann, J.F.

    1994-01-01

    The aim of this study was to investigate whether the cytoskeleton, and in particular the microtubular system, is affected by enhanced levels of ultraviolet-B (280–320 nm, 9 kJ m −2 day −1 biologically effective UV-B radiation) radiation in epidermal cells of Petunia x hybrida Vilm, isolated from leaves of plants grown under UV-B radiation and visible light. In addition, morphological changes during development were monitored. In a previous study microtubules were depolymerized and delays in the different stages of the cell cycle were found when protoplasts of Petunia were irradiated with UV-B radiation (Staxén et al. 1993. Protoplasma 173: 70–76). Thus it was of interest to ascertain whether the cytoskeleton would be similarly affected in an intact system. Assuming an effect of UV-B radiation on the microtubular system, we wished to determine whether this could be correlated to concomitant changes in leaf morphology. Plants of Petunia hybrida were grown in greenhouse conditions in the presence or absence of UV-B radiation. During the course of the experiment, samples were taken from young, expanding leaves and from older, fully expanded leaves and prepared for localization and analysis of microtubules from the adaxial epidermal cells. Morphology rather than the cytoskeleton was affected by UV radiation, despite the fact that the epidermal cytoskeleton would most likely be affected, since it is located in the cells which form the first intercepting layer for incident radiation. Morphological changes under UV-B radiation, as compared to those under control conditions, were reflected in earlier flowering and an increase in leaf number. Cell division was thus stimulated as was also evidenced from the increased leaf area. Our results indicate that the number of stomata differentiated on a leaf area basis was not altered although the number of stomata per epidermal cell was reduced. (author)

  13. 33 CFR 334.730 - Waters of Santa Rosa Sound and Gulf of Mexico adjacent to Santa Rosa Island, Air Force Proving...

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Waters of Santa Rosa Sound and..., Fla. 334.730 Section 334.730 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.730 Waters of Santa Rosa...

  14. Still the same after all these years: Santa Rosa`s curbside recycling model

    Energy Technology Data Exchange (ETDEWEB)

    Egan, K.

    1997-10-01

    When Santa Rosa, located north of San Francisco, first began its recycling program in 1977, it was no different from the other fledgling curbside recycling programs in the state. Back then, residents collected recyclables in their homes and put them out on the curb each week next to the garbage cans in whatever container they could find, whether it was a paper grocery bag or a cardboard box. The city`s pre-bin recycling program had a participation rate of about 25%. Then in 1978, with the firm`s help, Empire officials came up with the idea of providing recycling bins to residents. The program planners asked residents to source-separate their recyclables and then put the metals (including steel, tin, and aluminum); all three glass colors (brown, green, and clear); and newspaper in three individual bins. A few weeks after city officials distributed the bins to residents, the program showed a participation rate of 77%, indicating to Clark that the bins were necessary to significantly increase public awareness of the program.

  15. INTERSTOCK EFFECT ON THE GROWTH OF MANDARIN CV BATU 55, TANGERINE CV PONTIANAK AND LIME CV NIMAS PROPAGATED BY GRAFTING

    Directory of Open Access Journals (Sweden)

    Sugiyatno A.

    2017-10-01

    Full Text Available Japansche citroen (JC rootstock is still the main choice for producing citrus seeds for its high availability and compatibility. Other rootstocks have been used in the production, yet they produce unsatisfying results. Based on the theory, there is an alternative to use rootstock as interstock in order to stimulate the growth of scion. The objective of this reseach is to understand the effect of interstock on the growth of Mandarin cv. Batu 55, Tangerine cv. Pontianak and Lime cv. Nimas. This research was conducted in the screen house of Tlekung Experimental Field of Indonesian Citrus and Subtropical Fruits Research Institute (ICSFRI in Batu-East Java, located on 950 m asl, from January to April 2016. This research was arranged in a CRD design with 4 replications and 24 interstock-scion combination treatments. The interstocks used in this study were Carizzo citrange, Citrumello, Poncirus trifoliata, Volkameriana, Rough lemon, Troyer citrange, Kanci and control (JC combined with 3 scions namely Mandarin cv. Batu 55, Tangerine cv. Pontianak and Lime cv. Nimas propagated by grafting. The result showed the percentage of successful grafting ranged between 37.5% to 100%, while the percentage of the shoot formed on grafted plants was 75%-100%. The use of Poncirus trifoliata as interstock has stimulated fastest bud break on Tangerine cv. Pontianak in just 24.75 days while the Troyer citrange has stimulated better shoot growth in Lime cv. Nimas, resulted to the highest shoot around 52.20 cm which was significantly different from other treatments. Meanwhile, Carizzo citrange has been found to have some influences to the plant diameter and the number of leave of Lime cv. Nimas but showed insignificant effect on the growth of its own diameter. The use of interstock showed that it can be used to promote the growth of scion which usually has slow growth when it is directly grafted on the rootstock.

  16. Therapeutic Applications of Rose Hips from Different Rosa Species.

    Science.gov (United States)

    Mármol, Inés; Sánchez-de-Diego, Cristina; Jiménez-Moreno, Nerea; Ancín-Azpilicueta, Carmen; Rodríguez-Yoldi, María Jesús

    2017-05-25

    Rosa species, rose hips, are widespread wild plants that have been traditionally used as medicinal compounds for the treatment of a wide variety of diseases. The therapeutic potential of these plants is based on its antioxidant effects caused by or associated with its phytochemical composition, which includes ascorbic acid, phenolic compounds and healthy fatty acids among others. Over the last few years, medicinal interest in rose hips has increased as a consequence of recent research that has studied its potential application as a treatment for several diseases including skin disorders, hepatotoxicity, renal disturbances, diarrhoea, inflammatory disorders, arthritis, diabetes, hyperlipidaemia, obesity and cancer. In this review, the role of different species of Rosa in the prevention of treatment of various disorders related to oxidative stress, is examined, focusing on new therapeutic approaches from a molecular point of view.

  17. Tocopherols in rose hips (Rosa spp.) during ripening.

    Science.gov (United States)

    Andersson, Staffan C; Olsson, Marie E; Gustavsson, Karl-Erik; Johansson, Eva; Rumpunen, Kimmo

    2012-08-15

    Rose hips are used as a food ingredient and in health products. They are rich in various bioactive compounds such as carotenoids and vitamin C, but data on their vitamin E content (tocopherols and tocotrienols) are limited. In this study, four different species of Rosa were analysed for tocopherol and tocotrienol content during ripening in three different years. Only α- and γ-tocopherol were found in the fleshy parts of the rose hips, and the tocopherol content and vitamin E activity varied depending on date of harvesting, species and year. The amount of vitamin E activity differed between species of Rosa and years, whereas the changes during ripening were relatively small. The choice of species must be considered if tocopherol content is to be optimised when rose hips are used as a food ingredient. Copyright © 2012 Society of Chemical Industry.

  18. Mil rosas roubadas, Work of Silviano Santiago a Bakhitinian Analysis

    Directory of Open Access Journals (Sweden)

    Renata Coelho Marchezan

    2017-10-01

    Full Text Available This article analyzes Mil rosas roubadas, written by Silviano Santiago in 2014, dealing with the notions of (autobiography, (autobiographical novel, metafiction and autofiction. Although the creation of this last word may indicate the existence of a new genre – it is even adopted by the writer himself to name his work –, the article shows the pertinence of placing Mil rosas roubadas in the route of the transformations and stylisations of the (autobiographical novel, such as examined by M. Bakhtin. The novel is, for Bakhtin, a genre without rigid forms, an unfinished genre, as it always follows the inflections of social life. In this way the ressemantization of the self, proposed by Silviano Santiago, is analyzed.

  19. Rosa micrantha as a powerfull source of antioxidants

    OpenAIRE

    Guimarães, Rafaela; Barros, Lillian; Carvalho, Ana Maria; Ferreira, Isabel C.F.R.

    2010-01-01

    Reactive oxygen (ROS) and nitrogen (RNS) species production inevitably occurs during normal cell metabolism both in animals and plants. An excess of those species lead to oxidative and nitrosative stresses, resulting in damage for some cellular molecules such as DNA, proteins and lipids. Natural antioxidants have become a safety option to avoid biological oxidations involved in cellular damage and deterioration of food quality. Rosa micrantha is one of the rose species that gro...

  20. Analisis Kualitas Layanan Hotel New Sany Rosa Bandung

    OpenAIRE

    Elisabeth, Elisabeth

    2013-01-01

    In this decade, hospitality is an industry that grows rapidly in Indonesia, notably inBandung. New Sany Rosa Hotel Bandung is among the most popular hotels. Fac-ing tough industry, transformation at this hotel was enforced. The new managementdelivered a change in customer satisfaction valuation, from suggestions or criticsto service quality. The objective is to identify if five dimensions of service qualityimpact the customer satisfaction and which dimension has most influence on cus-tomer sa...

  1. Análisis de la evapotranspiración real en el cultivo de rosa

    OpenAIRE

    Esmeral Vargas, Yullayma Rosa

    2011-01-01

    La estimación de la evapotranspiración real, (ETc), para el cultivo de rosa (Rosa sp) constituye un elemento fundamental para lograr un uso eficiente del agua y un manejo adecuado del riego para el sector floricultor en Colombia. El objetivo de este trabajo fue determinar la ETc de Rosa (Rosa Sp) con técnicas de cultivo sin suelo bajo invernadero en la Sabana de Bogotá empleando el método de balance de agua diario. El diseño experimental fue completamente aleatorio. Se utilizaron tres tipos ...

  2. Storage and methyl jasmonate in postharvest conservation of roses cv. Avalanche

    Directory of Open Access Journals (Sweden)

    Elka Fabiana Aparecida Almeida

    2017-07-01

    Full Text Available The use of methyl jasmonate has demonstrated its efficiency to extend the vase life of cut flowers. The objective of this study was to evaluate the effect of methyl jasmonate associated with storage at low temperatures on the postharvest quality of Rosa cv. Avalanche stems. The treatments consisted of 125, 250, 500 and 1000 μM of methyl jasmonate, besides the control with distilled water. The flower buds were sprayed with 4 mL of the solution, according to the treatments, and then kept in a cold chamber (1 °C for periods of 2 and 6 days. Subsequently, the stems were taken to the postharvest laboratory at a temperature of 16 °C. Better quality, higher fresh weight and water absorption were observed in flower stems stored for 2 days. The application of methyl jasmonate caused less turgescence and greater darkening of roses. It was possible to conclude that two days is the best storage time at 1 °C and the use of methyl jasmonate does not maintain the quality of roses cv. Avalanche after harvest.

  3. Wyoming CV Pilot Traveler Information Message Sample

    Data.gov (United States)

    Department of Transportation — This dataset contains a sample of the sanitized Traveler Information Messages (TIM) being generated by the Wyoming Connected Vehicle (CV) Pilot. The full set of TIMs...

  4. cv. “Sukali Ndiizi”- AAB

    African Journals Online (AJOL)

    Clara

    2013-03-27

    Mar 27, 2013 ... improve transformation and regeneration efficiency of banana (cv. “Sukali .... Vuylsteke (1989) proliferation medium in the tissue culture .... DNA repair machinery is more active during cell division ... Biology-Plant 40:31-45.

  5. Population Dynamics of Macrosiphum rosae (L. on Different Cultivars of Rose (Rosahybrida, Rosaceae and Biodiversity of its Predators in Mashhad

    Directory of Open Access Journals (Sweden)

    M. Keykhosravi

    2016-06-01

    Full Text Available Introduction: Rose (Rosa hybrida (L., Rosaceae has been grown on earth for millions of years and has been used for beauty and decoration of gardens, extraction of perfume and in medicine. But main use of roses is in cut flower industry and landscaping. Roses are attractive for insects, especially aphids (2, 3, 5, 6, 7, 11, 17, 27, 28, 35, 38. Among them, the predominant aphid that feeds on the cultivated roses in outdoors is the rose aphid, Macrosiphum rosae (13, 27, 28, 29. The rose aphid has a wide distribution throughout Iran and the world (27, 28, 29. Rose aphids generally initiate feeding on roses in early spring as the new flush of growth emerges. Like other aphid species, rose aphids tend to congregate or cluster in large numbers feeding on the terminal growth including leaves and stems, and developing flower buds, and on leaf undersides. Their feeding causes deformity flower buds and leaves which may result in flower buds aborting or falling off prematurely before opening. In addition, aphids secrete honeydew, which attracts ants, wasps, hornets and serves as a growing medium for certain black sooty mold fungi. Rose aphids are attacked by anvarray of natural enemies including parasitoids and predators such as ladybird beetles, green lacewings, syrphids and several other groups of arthropods. These may provide natural regulation depending on the number of rose aphids present and other biotic and abiotic factors. Although many herbivorous arthropods may attack roses but many roses cultivar can resist against these pests (23, 26, 28, 40. An important factor influencing this success is careful selection of varieties, which vary significantly in susceptibility to pests and disease problems (9, 13, 19, 21, 23, 30, 40. Of course, other factors such as agricultural practices and the presence and activities of natural enemies of pests are also important. This study aimed to determine any resistance against rose aphid in different rose cultivars

  6. Chemical Composition and Antibacterial Activity of Iranian Lavandula × hybrida.

    Science.gov (United States)

    Bajalan, Iman; Rouzbahani, Razieh; Pirbalouti, Abdollah Ghasemi; Maggi, Filippo

    2017-07-01

    Lavandin (Lavandula × hybrida) is an evergreen shrub and cultivated worldwide for its essential oil which possesses various biological activities. In this study, the essential oils were isolated from the leaves of ten lavandin populations in western Iran. The hydrodistilled essential oils were analyzed by GC-FID/MS. Results indicated significant differences (P ≤ 0.05) among the various populations for the main essential oil constituents. The major components from different populations were 1,8-cineole (31.64 - 47.94%), borneol (17.11 - 26.14%), and camphor (8.41 - 12.68%). In vitro antibacterial activity was evaluated against S. agalactiae, S. aureus, E. coli, and K. pneumoniae. The inhibition zones were in the range of 09.36 mm for S. aureus to 23.30 mm for E. coli. Results indicated that there was a significant correlation between essential oil composition and level of antibacterial efficacy expressed as inhibition zones. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  7. Patterns of MADS-box gene expression mark flower-type development in Gerbera hybrida (Asteraceae

    Directory of Open Access Journals (Sweden)

    Teeri Teemu H

    2006-06-01

    Full Text Available Abstract Background The inflorescence of the cut-flower crop Gerbera hybrida (Asteraceae consists of two principal flower types, ray and disc, which form a tightly packed head, or capitulum. Despite great interest in plant morphological evolution and the tractability of the gerbera system, very little is known regarding genetic mechanisms involved in flower type specification. Here, we provide comparative staging of ray and disc flower development and microarray screening for differentially expressed genes, accomplished via microdissection of hundreds of coordinately developing flower primordia. Results Using a 9K gerbera cDNA microarray we identified a number of genes with putative specificity to individual flower types. Intrestingly, several of these encode homologs of MADS-box transcription factors otherwise known to regulate flower organ development. From these and previously obtained data, we hypothesize the functions and protein-protein interactions of several gerbera MADS-box factors. Conclusion Our RNA expression results suggest that flower-type specific MADS protein complexes may play a central role in differential development of ray and disc flowers across the gerbera capitulum, and that some commonality is shared with known protein functions in floral organ determination. These findings support the intriguing conjecture that the gerbera flowering head is more than a mere floral analog at the level of gene regulation.

  8. Investigation of callogenesis and indirect regeneration of Freesia × hybrida Bailey ‘Argenta’

    Directory of Open Access Journals (Sweden)

    Pourkhaloee Ali

    2015-12-01

    Full Text Available An investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (Freesia × hybrida Bailey ‘Argenta’. Sections of two different types of explants, corms and pupae (cold storage-produced corms, were placed on Murashige and Skoog (MS media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%, the highest callus growth (15 mm diameter and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−1 1-naphthaleneacetic acid (NAA and 2 mg L−1 6-benzylaminopurine (BAP in the dark. Increasing BAP up to 3 to 4.5 mg L−1 resulted in the maximum number of regenerated cormlets from 1 cm2 calli (2 cormlets under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1 indole-3-butyric acid (IBA. In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1. The results indicated that 15 g L−1 maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1 BAP and 1 mg L−1 NAA.

  9. Characterization of petunia flower mottle virus (PetFMV), a new potyvirus infecting Petunia x hybrida.

    Science.gov (United States)

    Feldhoff, A; Wetzel, T; Peters, D; Kellner, R; Krczal, G

    1998-01-01

    With the introduction of cutting-grown Petunia x hybrida plants on the European market, a new potyvirus which showed no serological reaction with antisera against any other potyviruses infecting petunias was discovered. Infected leaves contained flexuous rod-shaped virus particles of 750-800 nm in length and inclusion bodies (pinwheel structures) typical for potyviruses in ultrathin leaf sections. The purified coat protein with a Mr of approximately 36 kDa could be detected in Western immunoblots with a specific antibody to the coat protein of the petunia-infecting virus. The 3' end of the viral genome encompassing the 3' non-coding region, the coat protein gene, and part of the NIb gene was amplified from infected leaf material by IC/PCR using degenerate and specific primers. Sequences of PCR-generated cDNA clones were compared to other known sequences of potyviruses. Maximum homology of 56% was found in the 3' non-coding region between the petunia isolate and other potyviruses. A maximum homology of 69% was found between the amino acid sequence of the coat protein of the petunia isolate and corresponding sequences of other potyviruses. These data indicate that the petunia-infecting virus is a previously undescribed potyvirus and the name petunia flower mottle virus (PetFMV) is suggested.

  10. γ irradiation with different dose rates induces different DNA damage responses in Petunia x hybrida cells.

    Science.gov (United States)

    Donà, Mattia; Ventura, Lorenzo; Macovei, Anca; Confalonieri, Massimo; Savio, Monica; Giovannini, Annalisa; Carbonera, Daniela; Balestrazzi, Alma

    2013-05-15

    In plants, there is evidence that different dose rate exposures to gamma (γ) rays can cause different biological effects. The dynamics of DNA damage accumulation and molecular mechanisms that regulate recovery from radiation injury as a function of dose rate are poorly explored. To highlight dose-rate dependent differences in DNA damage, single cell gel electrophoresis was carried out on regenerating Petunia x hybrida leaf discs exposed to LDR (total dose 50 Gy, delivered at 0.33 Gy min(-1)) and HDR (total doses 50 and 100 Gy, delivered at 5.15 Gy min(-1)) γ-ray in the 0-24h time period after treatments. Significant fluctuations of double strand breaks and different repair capacities were observed between treatments in the 0-4h time period following irradiation. Dose-rate-dependent changes in the expression of the PhMT2 and PhAPX genes encoding a type 2 metallothionein and the cytosolic isoform of ascorbate peroxidase, respectively, were detected by Quantitative RealTime-Polymerase Chain Reaction. The PhMT2 and PhAPX genes were significantly up-regulated (3.0- and 0.7-fold) in response to HDR. The results are discussed in light of the potential practical applications of LDR-based treatments in mutation breeding. Copyright © 2013 Elsevier GmbH. All rights reserved.

  11. Phenylpropanoid Scent Compounds in Petunia x hybrida Are Glycosylated and Accumulate in Vacuoles

    Science.gov (United States)

    Cna'ani, Alon; Shavit, Reut; Ravid, Jasmin; Aravena-Calvo, Javiera; Skaliter, Oded; Masci, Tania; Vainstein, Alexander

    2017-01-01

    Floral scent has been studied extensively in the model plant Petunia. However, little is known about the intracellular fate of scent compounds. Here, we characterize the glycosylation of phenylpropanoid scent compounds in Petunia x hybrida. This modification reduces scent compounds' volatility, reactivity, and autotoxicity while increasing their water-solubility. Gas chromatography–mass spectrometry (GC–MS) analyses revealed that flowers of petunia cultivars accumulate substantial amounts of glycosylated scent compounds and that their increasing level parallels flower development. In contrast to the pool of accumulated aglycones, which drops considerably at the beginning of the light period, the collective pool of glycosides starts to increase at that time and does not decrease thereafter. The glycoside pool is dynamic and is generated or catabolized during peak scent emission, as inferred from phenylalanine isotope-feeding experiments. Using several approaches, we show that phenylpropanoid scent compounds are stored as glycosides in the vacuoles of petal cells: ectopic expression of Aspergillus niger β-glucosidase-1 targeted to the vacuole resulted in decreased glycoside accumulation; GC–MS analysis of intact vacuoles isolated from petal protoplasts revealed the presence of glycosylated scent compounds. Accumulation of glycosides in the vacuoles seems to be a common mechanism for phenylpropanoid metabolites. PMID:29163617

  12. Petunia × hybrida floral scent production is negatively affected by high-temperature growth conditions.

    Science.gov (United States)

    Cna'ani, Alon; Mühlemann, Joelle K; Ravid, Jasmin; Masci, Tania; Klempien, Antje; Nguyen, Thuong T H; Dudareva, Natalia; Pichersky, Eran; Vainstein, Alexander

    2015-07-01

    Increasing temperatures due to changing global climate are interfering with plant-pollinator mutualism, an interaction facilitated mainly by floral colour and scent. Gas chromatography-mass spectroscopy analyses revealed that increasing ambient temperature leads to a decrease in phenylpropanoid-based floral scent production in two Petunia × hybrida varieties, P720 and Blue Spark, acclimated at 22/16 or 28/22 °C (day/night). This decrease could be attributed to down-regulation of scent-related structural gene expression from both phenylpropanoid and shikimate pathways, and up-regulation of a negative regulator of scent production, emission of benzenoids V (EOBV). To test whether the negative effect of increased temperature on scent production can be reduced in flowers with enhanced metabolic flow in the phenylpropanoid pathway, we analysed floral volatile production by transgenic 'Blue Spark' plants overexpressing CaMV 35S-driven Arabidopsis thaliana production of anthocyanin pigments 1 (PAP1) under elevated versus standard temperature conditions. Flowers of 35S:PAP1 transgenic plants produced the same or even higher levels of volatiles when exposed to a long-term high-temperature regime. This phenotype was also evident when analysing relevant gene expression as inferred from sequencing the transcriptome of 35S:PAP1 transgenic flowers under the two temperature regimes. Thus, up-regulation of transcription might negate the adverse effects of temperature on scent production. © 2014 John Wiley & Sons Ltd.

  13. Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida.

    Science.gov (United States)

    Bombarely, Aureliano; Moser, Michel; Amrad, Avichai; Bao, Manzhu; Bapaume, Laure; Barry, Cornelius S; Bliek, Mattijs; Boersma, Maaike R; Borghi, Lorenzo; Bruggmann, Rémy; Bucher, Marcel; D'Agostino, Nunzio; Davies, Kevin; Druege, Uwe; Dudareva, Natalia; Egea-Cortines, Marcos; Delledonne, Massimo; Fernandez-Pozo, Noe; Franken, Philipp; Grandont, Laurie; Heslop-Harrison, J S; Hintzsche, Jennifer; Johns, Mitrick; Koes, Ronald; Lv, Xiaodan; Lyons, Eric; Malla, Diwa; Martinoia, Enrico; Mattson, Neil S; Morel, Patrice; Mueller, Lukas A; Muhlemann, Joëlle; Nouri, Eva; Passeri, Valentina; Pezzotti, Mario; Qi, Qinzhou; Reinhardt, Didier; Rich, Melanie; Richert-Pöggeler, Katja R; Robbins, Tim P; Schatz, Michael C; Schranz, M Eric; Schuurink, Robert C; Schwarzacher, Trude; Spelt, Kees; Tang, Haibao; Urbanus, Susan L; Vandenbussche, Michiel; Vijverberg, Kitty; Villarino, Gonzalo H; Warner, Ryan M; Weiss, Julia; Yue, Zhen; Zethof, Jan; Quattrocchio, Francesca; Sims, Thomas L; Kuhlemeier, Cris

    2016-05-27

    Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 and 36,697 protein-coding genes, respectively. The genomes reveal that the Petunia lineage has experienced at least two rounds of hexaploidization: the older gamma event, which is shared with most Eudicots, and a more recent Solanaceae event that is shared with tomato and other solanaceous species. Transcription factors involved in the shift from bee to moth pollination reside in particularly dynamic regions of the genome, which may have been key to the remarkable diversity of floral colour patterns and pollination systems. The high-quality genome sequences will enhance the value of Petunia as a model system for research on unique biological phenomena such as small RNAs, symbiosis, self-incompatibility and circadian rhythms.

  14. Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes.

    Science.gov (United States)

    Sasse, Joëlle; Schlegel, Markus; Borghi, Lorenzo; Ullrich, Friederike; Lee, Miyoung; Liu, Guo-Wei; Giner, José-Luis; Kayser, Oliver; Bigler, Laurent; Martinoia, Enrico; Kretzschmar, Tobias

    2016-12-01

    As a first line of defense against insect herbivores many plants store high concentrations of toxic and deterrent secondary metabolites in glandular trichomes. Plant Pleiotropic Drug Resistance (PDR)-type ABC transporters are known secondary metabolite transporters, and several have been implicated in pathogen or herbivore defense. Here, we report on Petunia hybrida PhPDR2 as a major contributor to trichome-related chemical defense. PhPDR2 was found to localize to the plasma membrane and be predominantly expressed in multicellular glandular trichomes of leaves and stems. Down-regulation of PhPDR2 via RNA interference (pdr2) resulted in a markedly higher susceptibility of the transgenic plants to the generalist foliage feeder Spodoptera littoralis. Untargeted screening of pdr2 trichome metabolite contents showed a significant decrease in petuniasterone and petuniolide content, compounds, which had previously been shown to act as potent toxins against various insects. Our findings suggest that PhPDR2 plays a leading role in controlling petuniasterone levels in leaves and trichomes of petunia, thus contributing to herbivory resistance. © 2016 John Wiley & Sons Ltd.

  15. The effect of Medicago arabica, M. hybrida and M. sativa saponins on the growth and development of Fusarium oxysporum Schlecht f. sp. tulipae apt.

    Directory of Open Access Journals (Sweden)

    Anna Jarecka

    2012-12-01

    Full Text Available In the present work it was shown that total saponins originated from M. hybrida and M. sativa substantially limited mycelium growth of F. oxysporum f. sp. tulipae and symptoms of fusariosis on tulip bulbs. Out of 15 individual tested saponins originated from M. arabica, M. hybrida and M. sativa, four compounds: 3-O-[β-D-glucopyranosyl (1→2α-L-arabinopyranosyl] hederagenin, hederagenin 3-O-β-D-glucopyranoside, medicagenic acid, medicagenic acid 3-O-β-D-glucopyranoside had the strongest inhibitory effect on mycelium growth of Fusarium oxysporum f. sp. tulipae on PDA medium. The total saponins from M. arabica, M. hybrida and M. sativa inhibited the number of colony forming units of Fusarium oxysporum f. sp. tulipae in artificially infested substrate. The use of saponins originated from Medicago as a fungicide is suggested.

  16. Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening

    OpenAIRE

    Xue, Jingqi; Li, Yunhui; Tan, Hui; Yang, Feng; Ma, Nan; Gao, Junping

    2008-01-01

    Ethylene production, as well as the expression of ethylene biosynthetic (Rh-ACS1?4 and Rh-ACO1) and receptor (Rh-ETR1?5) genes, was determined in five different floral tissues (sepals, petals, stamens, gynoecia, and receptacles) of cut rose (Rosa hybrida cv. Samantha upon treatment with ethylene or the ethylene inhibitor 1-methylcyclopropene (1-MCP). Ethylene-enhanced ethylene production occurred only in gynoecia, petals, and receptacles, with gynoecia showing the greatest enhancement in the ...

  17. De roodrandzandbij Andrena rosae in de Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae).

    NARCIS (Netherlands)

    Meer, van der F.; Reemer, M.; Peeters, T.M.J.

    2006-01-01

    Andrena rosae in the Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae) Andrena rosae is a fairly large, black and red mining bee. It has declined dramatically in the 20th century in northwestern Europe. In the Netherlands the species only survived in and around the Biesbosch, at the

  18. «Magma»: as origens de Guimarães Rosa

    Directory of Open Access Journals (Sweden)

    Luiz Cláudio Vieira de Oliveira

    2011-10-01

    Full Text Available Resumo: Leitura de Magma, de Guimarães Rosa, com o objetivo de indicar a presença de temas, fragmentos, personagens, expressões e recursos estilísticos ali existentes, em outros textos do autor, cronologicamente posteriores.Palavras-chave: Literatura brasileira; Guimarães Rosa; Magma.Résumé: Lecture de Magma, de Guimarães Rosa, ayant l’objectif de montrer la présence de quelques sujets, fragments, personnages, expressions et traits stylistiques, que y sont présents, et aussi dans autres textes du même auteur, chronologiquement postérieurs.Mots-clés: Littérature brésilienne; Guimarães Rosa; Magma.Keywords: Brazilian literature; Guimarães Rosa; Magma.

  19. Face and Convergent Validity of Persian Version of Rapid Office Strain Assessment (ROSA Checklist

    Directory of Open Access Journals (Sweden)

    Afrouz Armal

    2016-01-01

    Full Text Available Objective: The aim of this work was the translation, cultural adaptation and validation of the Persian version of the Rapid Office Stress Assessment (ROSA checklist. Material & Methods: This methodological study was conducted according of IQOLA method. 100 office worker were selected in order to carry out a psychometric evaluation of the ROSA checklist by performing validity (face and convergent analyses. The convergent validity was evaluated using RULA checklist. Results: Upon major changes made to the ROSA checklist during the translation/cultural adaptation process, face validity of the Persian version was obtained. Spearman correlation coefficient between total score of ROSA check list and RULA checklist was significant (r=0.76, p<0.0001. Conclusion: The results indicated that the translated version of the ROSA checklist is acceptable in terms of face validity, convergent validity in target society, and hence provides a useful instrument for assessing Iranian office workers

  20. The far East in the Poetry of Rosa Romojaro

    Directory of Open Access Journals (Sweden)

    Yanghongyi Ou

    2016-05-01

    Full Text Available The present article pays attention, in the first place, to the new conditions which provide a wide reception of the East Asian literature in the western area. Coming up next, in this context, a study of the poetries of Rosa Romojaro has been carried out, from the key points and compared motives, especially with the Chinese lyrical poetries and its tradition. After presenting the creative work of Rosa Romojaro, one of the most outstanding Spanish poets nowadays, standing on the point of view of a Chinese original critic, fundamental motives are given in advance in the chosen texts, and constantly linking with the Asian canon: so as the nature, and the degree of the symbolic subtlety in the writing of the poet according to the eastern point of view; the feeling of “Hen”, which contains the emotion of bittersweet and also associates with the passage of time; the essence of the poems and every word of hers, which is fulfilled with sentiments; the symbolism of the moon and its metaphorical reflections of the nocturne; the great motive of voyage and motions, and attached to them, the hope and the seasons, the fall; the adventure of  discovery within the heart of the known places and the purity driven by her glance; the synesthetic evocations; and, lastly, the relationship of her poems with the vital prolongation, making up every poem of Rosa Romojaro in a center of expressivity shared with universal motives which look up to the tradition and the modernity.  

  1. Geophysics report of Santa Rosa place Canelones province

    International Nuclear Information System (INIS)

    Cicalese, H.

    1984-01-01

    The Hydrogeology Section required to Geophysics Department of the DINAMIGE to carry out this report for Education and Culture Ministery, to study the feasibility of the exploration and exploitation of underground waters located in three properties zones in Santa Rosa's town, Canelones province. By means of the geoeletric methods it was possible to estimate the alluviums thickness ,sedimentary deposits or alteration mantels. The purpose of the present work has been to establish geologic and structural features through vertical electric well by means of which is been able to study the vertical variations of the resistivity .

  2. Morphological and chemical characteristics of fruits of selected Rosa sp.

    Directory of Open Access Journals (Sweden)

    Agnieszka Najda

    2013-04-01

    Full Text Available The aim of this study was to evaluate the morphological and chemical parameters, antioxidant activity (DPPH of five rose species (Rosa rugosa, R. villosa, R. californica, R. spinosissima, and R. × damascene and grouping them according to the harvest date. On the basis of the study, rose species grown in eastern Poland were grouped according to their harvest time, and three dates of cumulative ripeness of pseudofruits were distinguished. Rosehips of studied species varied referring to their harvest date and morphological properties and were characterized by diverse contents of primary metabolites analyzed. In addition, obtained extracts showed high antioxidant activity, which has a significant impact on their value for processing.

  3. Evaluating and validating the protocol for gamma radiation induced mutations in floral distinct rosa spp

    International Nuclear Information System (INIS)

    Qadeer, M.; Hafiz, I. A.; Abbasi, N. A.; Ahmad, T.

    2015-01-01

    Among the highly fragrant Rosa species, Rosa gruss an teplitz and Rosa centifolia have high value in terms of commercial importance and economical trade. However, the absence of floral diversification and diversity in color patterns in these species acts as limiting factors in floriculture trade of these species. In the present study, we aimed to induce mutations using gamma radiations up to 120 Gy to observe the rate and the correlated effects on the several plant traits in micropropagated shoot tips of Rosa gruss an teplitz and Rosa centifolia. Irradiated shoot tips were micropropagated for one culture cycle and were acclimatized in a green house after in vitro rooting. Plants of irradiated population at 60 and 30 Gy showed 78.12 and 38.50 percentage less culture rooting percentage age as well as 23.82 and 7 percentage less flower size as compared to non irradiated population of Rosa gruss an teplitz and Rosa centifolia respectively. Moreover, flower color component a* (+ redness - green, 36.16 and 27.16) and chroma (37.77 and 27.5) depicted minimum while L* (Lightness, 45.12 and 76.64), b* (+ yellow - blue, 10.69 and -4.14) and hue angle (17.1 and -8.58) maximum value. Apart from these variations, mutants of Rosa gruss an teplitz also produced variegated, pink color and different shape flowers. Genetic variations observed among the putative mutants of Rosa gruss an teplitz and Rosa centifolia were evaluated using twelve decamer RAPD primers. Phylogenetic inferences showed large genetic diversity in putative mutants as compared to mother plant. (author)

  4. Role of the GRAS transcription factor ATA/RAM1 in the transcriptional reprogramming of arbuscular mycorrhiza in Petunia hybrida

    OpenAIRE

    Rich, Melanie K.; Courty, Pierre-Emmanuel; Roux, Christophe; Reinhardt, Didier

    2017-01-01

    Background Development of arbuscular mycorrhiza (AM) requires a fundamental reprogramming of root cells for symbiosis. This involves the induction of hundreds of genes in the host. A recently identified GRAS-type transcription factor in Petunia hybrida, ATA/RAM1, is required for the induction of host genes during AM, and for morphogenesis of the fungal endosymbiont. To better understand the role of RAM1 in symbiosis, we set out to identify all genes that depend on activation by RAM1 in mycorr...

  5. Floral pipe: length in Petunia x hybrida heredability, number of genes and the interaction of the date with the character

    OpenAIRE

    Fatta, N.; Vazquez, M.; García, N.; Mascarini, A.; Grigioni, G.

    2007-01-01

    Petunia x hybrida, a floral summer of a economical importance in Buenos Aires, is nowadays produced with imported seed. The research presented is focused on the study of the feasibility to obtain locally competitive seed. The length of the floral pipe is a relevant character so the assay was designed in order to estimate the heredability, the number of genes intervener and the interactions with the date. The assay began with a F1 purchased from United States. In 2003, the F1 and F2 were sowed...

  6. Propiedades físicas e hidráulicas de perlita en cultivos de rosas y sus variaciones temporales Physical and hydraulic properties of perlites used in rose cultures and their dependency on time

    Directory of Open Access Journals (Sweden)

    Daniel A. Martínez

    2006-12-01

    Full Text Available Se evaluaron las características físicas e hidráulicas de la perlita usada como sustrato único, en un cultivo bajo cubierta de rosas (Rosa hybrida L. para corte, observando su comportamiento durante el cultivo. Se evaluaron muestras de perlita con 0, 28 y 39 meses de uso extraídas de la zona radicular del cultivo. Se determinaron la densidad aparente (DA, densidad real de las partículas intactas (DRI, densidad real de las partículas pulverizadas (DRP, distribución de tamaño de partícula, capacidad de aire (CA, agua fácilmente disponible (AF, agua de reserva (AR, porosidad efectiva (PE. La conductividad hidráulica no saturada (Kns se obtuvo a partir del modelo de Mualem-van Genuchten. La perlita registró en el tiempo, rotura de los granos superiores a 2 mm, observándose un incremento de la fracción comprendida entre 1 mm y 2 mm, no habiendo modificaciones significativas en partículas inferiores a 0,5 mm. Se observó un aumento de la PE, y de la CA mientras que los valores de AF, AR se mantuvieron aproximadamente constantes. El aumento de PE podría explicarse como resultado de la inserción de las raíces y el arrastre de las partículas finas fuera de la zona radicular. En cambio, de forma consistente con el aumento de la CA, Kns experimentó una disminución en un orden de magnitud luego de 39 meses.Physical and hydraulic properties of perlite used as unique substrate in a greenhouse cut roses (Rosa hybrida L. culture and their dependence on time were investigated. Samples of, perlite with 0,28 and 39 months of use, extracted from the rhizosphere, were analyzed. Bulk density (DA, real density of intact particles (DRI real density of pulverized particles (DRP, particle size distribution, air capacity (CA, available water (AFD, unavailable water (AR and effective porosity (PE were determined. Non-saturated hydraulic conductivity (Kns was obtained using the Mualemvan Genuchten model. It was found cracking of particles with

  7. Diversity of morphology and oil content of rosa damascena land races and related rosa species from Pakistan

    International Nuclear Information System (INIS)

    Farooq, A.; Khan, M.; Riaz, A.; Ali, A.

    2011-01-01

    For the perfume industry, Rosa damascena is the most important species used in the production of rose attar which is made by distilling volatile oils from the petals of flowers. It is also used widely in the production of rose water, a flavoring agent. Other species like R. gallica L., R. centifolia L., R. bourboniana and Gruss an Teplitz also exhibit the fragrance that is sought by perfumeries in the world. Eight land races of Damask rose along with related Rosa species were collected from Punjab province and evaluated to determine the diversity on the basis of morphology and oil yield. The investigated characters were flower weight, flower diameter, peduncle length, number of petals, number of stamens and oil content. Pearson's coefficients showed a negative association of flower weight with peduncle length (r = -0.3348) and a positive and strong correlation was observed for flower weight with the all other morphological characters. Flower diameter showed a positive but weak correlation with peduncle length, number of petals and number of stamens with the values of r = 0.0733, r 0.5302 and r = 0.1241, respectively. Oil content (%) was measured from the Rosa species by using Soxhlet extractor with N-hexane. R. damascena land race from Choha Syedan Shah produced the highest oil content of absolute oil (0.147%) on petal basis while the lowest value for absolute oil content was 0.36% from R. indica. Dendrogram created by cluster analysis for morphological characters, indicated no relationship of genetic variation with their collection sites. This study has revealed that the selection of land races for their specific characteristic could be possible for future breeding program. It also provides practical information for the future collection of Damask rose germplasm and breeding program. (author)

  8. Analysis of MINT hibiscus rosa-sinensis mutants

    International Nuclear Information System (INIS)

    Shakinah Salleh; Shuhaimi Shamsudin; Putri Noor Faizah MM Tahir; Mohd Nazir Basiran

    2006-01-01

    Gamma irradiation is an alternative method to create new cultivars of Hibiscus rosa-sinensis in Malaysia. Increasing gamma ray doses resulted in a reduction of growing shoots on the irradiated stem cuttings. The LD50 for the stem cuttings was 36.2 Gy and at 75% growth was 17.15 Gy. Based on these results, 20 to 30 Gy were chosen for irradiation of the stem cuttings to induce mutation in Hibiscus rosa-sinensis. Irradiated stem cuttings were then planted into sand-beds for rooting. After one month, the rooted stems were transferred into polybags and allowed to grow under 70% shade provided by plastic netting and the variants were subsequently observed. Three new cultivars with variations in flower size and colours were obtained. The plants were propagated for five generations to achieve the stable mutants. Anthocyanins analysis is being carried out to measure the amount of anthocyanins in each cultivar. Pelargonidin chloride, Cyanidin chloride and Delphinidin chloride are used as standards for the analysis. (Author)

  9. Experiment data of ROSA-III integral test Run 7341

    International Nuclear Information System (INIS)

    Anoda, Yoshinari; Soda, Kunihisa; Tasaka, Kanji

    1983-02-01

    This report presents the test data of Run 7341 in the single failure test series of the ROSA-III program to conduct the system effect test concerning the response of a BWR during a LOCA with the ECC injection. The ROSA-III test facility is a volumetrically scaled (1/424) system of the BWR/6 with an electrically heated core and the scaled ECCS. Run 7341 is a double-ended break test at the recirculation pump inlet with the assumption that all ECCS function as designed. The test is initiated with the steam dome pressure of 7.28 MPa, the lower plenum subcooling of 11.0 K, the core inlet flow rate of 15.3 kg/s, and the core heat generation rate of 3.55 MW and proceeded as planned. The whole core is quenched after the ECCS actuation and the maximum fuel cladding temperature is 810 K. The effectiveness of ECC injection has been clarified from the comparison of the test results with those of the other test in the series without ECCS. (author)

  10. CORÍN TELLADO Y LA NOVELA ROSA

    Directory of Open Access Journals (Sweden)

    Encarna Alonso Valero

    2012-07-01

    Full Text Available Resumen: Las novelas de Corín Tellado marcaron la vida de varias generaciones de mujeres y también de muchos hombres. Sufrió los reproches habituales a la novela comercial y de éxito, además del desprecio dedicado a la llamada novela rosa al ser considerada un género menor y fundamentalmente para mujeres. En este artículo nos proponemos analizar cómo con frecuencia ese tipo de juicios no son literarios sino sociales, juicios sociales denegados. Además, estudiaremos las novelas de Corín Tellado, que influyeron enormemente en la “educación sentimental” de las mujeres durante el franquismo, y analizaremos el modelo de mujer que presentan. Abstract: The novels of Corín Tellado marked the life of several generations of women, as well as of many men. She suffered the criticisms habitually levelled at commercially successful novels, especially the so-called novela rosa, or popular romance novel, because of its reputation as a minor genre aimed primarily at women. This paper aims to show that these judgments are often not literary but social. The paper will also examine Corín Tellado's novels, which greatly influenced the 'sentimental education' of women during the Franco regime, and we will analyze the models of woman that theses romances present.

  11. Estimation of Heterosis and Combining Ability in Petunia (Petunia hybrida Hort.

    Directory of Open Access Journals (Sweden)

    Hassan BAYAT

    2012-08-01

    Full Text Available Combining ability and heterosis were estimated for ornamental and vegetative traits of four petunia (Petunia hybrida Hort. inbred lines viz. L5 (P1, L8 (P2, L11 (P3 and L17 (P4 and their diallel hybrids in horticultural research farm of Ferdowsi University of Mashhad, Iran in 2011. The measured traits were plant height at first flower, number of leaf to first flower, flower diameter, flower tube length, internode length, stem diameter, plant height at flowering stage, plant spreading, number of branches per plant, leaf length and width. The results of combining ability for all studied traits revealed that both additive and non-additive gene effects contributed to the inheritance of the traits. Estimates of general combining ability effects showed that parent P3 was a good general combiner for most of the studied traits. For flower diameter, hybrid combination P1 � P2 had the highest significant positive specific combining ability effects. Reciprocal effects were significant for all traits and hybrid combination P1 � P2 had the highest significant positive reciprocal effects for flower tube length and plant height. Heterosis was found significant relative to both the mid parent and batter parent for all traits. For flower diameter, the highest positive values of heterotic effects were recorded in hybrid combination P2 � P3 both relative to the parental mean (37.3% and relative to the better parent (33.9%. It is obvious that heterotic effects represent an important resource in hybrid breeding of petunia.

  12. Estimation of Heterosis and Combining Ability in Petunia (Petunia hybrida Hort.

    Directory of Open Access Journals (Sweden)

    Hassan BAYAT

    2012-08-01

    Full Text Available Combining ability and heterosis were estimated for ornamental and vegetative traits of four petunia (Petunia hybrida Hort. inbred lines viz. L5 (P1, L8 (P2, L11 (P3 and L17 (P4 and their diallel hybrids in horticultural research farm of Ferdowsi University of Mashhad, Iran in 2011. The measured traits were plant height at first flower, number of leaf to first flower, flower diameter, flower tube length, internode length, stem diameter, plant height at flowering stage, plant spreading, number of branches per plant, leaf length and width. The results of combining ability for all studied traits revealed that both additive and non-additive gene effects contributed to the inheritance of the traits. Estimates of general combining ability effects showed that parent P3 was a good general combiner for most of the studied traits. For flower diameter, hybrid combination P1 P2 had the highest significant positive specific combining ability effects. Reciprocal effects were significant for all traits and hybrid combination P1 P2 had the highest significant positive reciprocal effects for flower tube length and plant height. Heterosis was found significant relative to both the mid parent and batter parent for all traits. For flower diameter, the highest positive values of heterotic effects were recorded in hybrid combination P2 P3 both relative to the parental mean (37.3% and relative to the better parent (33.9%. It is obvious that heterotic effects represent an important resource in hybrid breeding of petunia.

  13. Manipulation of MKS1 gene expression affects Kalanchoë blossfeldiana and Petunia hybrida phenotypes.

    Science.gov (United States)

    Gargul, Joanna Maria; Mibus, Heiko; Serek, Margrethe

    2015-01-01

    The establishment of alternative methods to chemical treatments for growth retardation and pathogen protection in ornamental plant production has become a major goal in recent breeding programmes. This study evaluates the effect of manipulating MAP kinase 4 nuclear substrate 1 (MKS1) expression in Kalanchoë blossfeldiana and Petunia hybrida. The Arabidopsis thaliana MKS1 gene was overexpressed in both species via Agrobacterium-mediated transformation, resulting in dwarfed phenotypes and delayed flowering in both species and increased tolerance to Pseudomonas syringae pv. tomato in transgenic Petunia plants. The lengths of the stems and internodes were decreased, while the number of nodes in the transgenic plants was similar to that of the control plants in both species. The transgenic Kalanchoë flowers had an increased anthocyanin concentration, and the length of the inflorescence stem was decreased. The morphology of transgenic Petunia flowers was not altered. The results of the Pseudomonas syringae tolerance test showed that Petunia plants with one copy of the transgene reacted similarly to the nontransgenic control plants; however, plants with four copies of the transgene exhibited considerably higher tolerance to bacterial attack. Transgene integration and expression was determined by Southern blot hybridization and RT-PCR analyses. MKS1 in wild-type Petunia plants was down-regulated through a virus-induced gene silencing (VIGS) method using tobacco rattle virus vectors. There were no significant phenotypic differences between the plants with silenced MKS1 genes and the controls. The relative concentration of the MKS1 transcript in VIGS-treated plants was estimated by quantitative RT-PCR. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  14. Extraction and the Fatty Acid Profile of Rosa acicularis Seed Oil.

    Science.gov (United States)

    Du, Huanan; Zhang, Xu; Zhang, Ruchun; Zhang, Lu; Yu, Dianyu; Jiang, Lianzhou

    2017-12-01

    Rosa acicularis seed oil was extracted from Rosa acicularis seeds by the ultrasonic-assisted aqueous enzymatic method using cellulase and protease. Based on a single experiment, Plackett-Burman design was applied to ultrasonic-assisted aqueous enzymatic extraction of wild rose seed oil. The effects of enzyme amount, hydrolysis temperature and initial pH on total extraction rate of wild rose seed oil was studied by using Box-Behnken optimize methodology. Chemical characteristics of a sample of Rosa acicularis seeds and Rosa acicularis seed oil were characterized in this work. The tocopherol content was 200.6±0.3 mg/100 g oil. The Rosa acicularis seed oil was rich in linoleic acid (56.5%) and oleic acid (34.2%). The saturated fatty acids included palmitic acid (4%) and stearic acid (2.9%). The major fatty acids in the sn-2 position of triacylglycerol in Rosa acicularis oil were linoleic acid (60.6%), oleic acid (33.6%) and linolenic acid (3.2%). According to the 1,3-random-2-random hypothesis, the dominant triacylglycerols were LLL (18%), LLnL (1%), LLP (2%), LOL (10%), LLSt (1.2%), PLP (0.2%), LLnP (0.1%), LLnO (0.6%) and LOP (1.1%). This work could be useful for developing applications for Rosa acicularis seed oil.

  15. Development and evaluation of an office ergonomic risk checklist: ROSA--rapid office strain assessment.

    Science.gov (United States)

    Sonne, Michael; Villalta, Dino L; Andrews, David M

    2012-01-01

    The Rapid Office Strain Assessment (ROSA) was designed to quickly quantify risks associated with computer work and to establish an action level for change based on reports of worker discomfort. Computer use risk factors were identified in previous research and standards on office design for the chair, monitor, telephone, keyboard and mouse. The risk factors were diagrammed and coded as increasing scores from 1 to 3. ROSA final scores ranged in magnitude from 1 to 10, with each successive score representing an increased presence of risk factors. Total body discomfort and ROSA final scores for 72 office workstations were significantly correlated (R = 0.384). ROSA final scores exhibited high inter- and intra-observer reliability (ICCs of 0.88 and 0.91, respectively). Mean discomfort increased with increasing ROSA scores, with a significant difference occurring between scores of 3 and 5 (out of 10). A ROSA final score of 5 might therefore be useful as an action level indicating when immediate change is necessary. ROSA proved to be an effective and reliable method for identifying computer use risk factors related to discomfort. Copyright © 2011 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  16. OpenCV for secret agents

    CERN Document Server

    Howse, Joseph

    2015-01-01

    This book is for programmers who want to expand their skills by building fun, smart, and useful systems with OpenCV. The projects are ideal in helping you to think creatively about the uses of computer vision, natural user interfaces, and ubiquitous computers (in your home, car, and hand).

  17. AstroCV: Astronomy computer vision library

    Science.gov (United States)

    González, Roberto E.; Muñoz, Roberto P.; Hernández, Cristian A.

    2018-04-01

    AstroCV processes and analyzes big astronomical datasets, and is intended to provide a community repository of high performance Python and C++ algorithms used for image processing and computer vision. The library offers methods for object recognition, segmentation and classification, with emphasis in the automatic detection and classification of galaxies.

  18. Management of arbuscular mycorrhizal fungi by growing petunia hybrida (l.) mill. as an ornamental plant in saudi arabia - a case study

    International Nuclear Information System (INIS)

    Qarawi, A.A.; Mridha, M.A.U.; Alghamdi, O.M.

    2014-01-01

    Arbuscular mycorrhizal fungi (AMF) regarded as ubiquitous soil fungi which help in improving plant growth under harsh conditions. Petunia hybrida is one of the most favorite ornamental plants growing all over the Riyadh city of Saudi Arabia. In the present study, we would like to highlight the Petunia as a mycotrophic plant for the management of mycorrhizal fungi under field conditions. Roots along with rhizosphere soils of P. hybrida were collected from various sites in Riyadh, Saudi Arabia to study AM colonization and biodiversity of AMF. The data obtained in this study indicated that P. hybrida is a very highly mycotrophic plants, and all the samples produced very high colonization with mycelium, vesicles, coiled hyphae and arbuscules. The significant variation was found with the occurrence of mycelium and vesicles among the locations but in case of arbuscules more or less same range of occurrence was found. Only different species of Glomus were observed in all the locations. Glomus showed diversity in all the locations as indicated by the Shanon Diversity Index. As the P. hybrida is a highly mycotrophic plant, so this plant may be grown under harsh condition of Saudi Arabia to manage the plant growth under different stresses viz., water stress, saline soils and heavy metal toxicity conditions. (author)

  19. Two potential Petunia hybrida mitochondrial DNA replication origins show structural and in vitro functional homology with the animal mitochondrial DNA heavy and light strand replication origins

    NARCIS (Netherlands)

    Haas, Jan M. de; Hille, Jacques; Kors, Frank; Meer, Bert van der; Kool, Ad J.; Folkerts, Otto; Nijkamp, H. John J.

    1991-01-01

    Four Petunia hybrida mitochondrial (mt) DNA fragments have been isolated, sequenced, localized on the physical map and analyzed for their ability to initiate specific DNA synthesis. When all four mtDNA fragments were tested as templates in an in vitro DNA synthesizing lysate system, developed from

  20. El duelo y el tiempo mítico en Rosa Cuchillo y La hora azul

    OpenAIRE

    Torres Oyarce, Tania

    2017-01-01

    In this paper, I propose that the novels Rosa Cuchillo and La hora azulproject individual processes of mourning in a mythical social order thatlimits itself to reproduce the phantom of the enclosed nation (Ubilluz) asa (seudo) proposal to solve the internal conflict that took place in Perubetween 1980-2000. In Rosa Cuchillo the main character, Rosa Cuchillo,goes through a process of mourning due to the loss of his terrorist son,Liborio, whereas in La hora azul the main character, Adrián Ormac...

  1. El duelo y el tiempo mítico en Rosa Cuchillo y La hora azul

    OpenAIRE

    Torres Oyarce, Tania

    2017-01-01

    En este artículo, sostenemos que las novelas Rosa Cuchillo y La hora azul proyectan procesos individuales de duelo en un orden social mítico que se limita a reproducir el fantasma de la nación cercada (Ubilluz) como (seudo) propuesta de solución al conflicto armado acontecido en el Perú entre 1980-2000. En Rosa Cuchillo, la protagonista Rosa Cuchillo atraviesa un proceso de duelo por haber perdido a su hijo subversivo, Liborio, mientras que, en La hora azul, el protagonista Adrián Ormache suf...

  2. The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12

    Directory of Open Access Journals (Sweden)

    Jiliang Fang

    2012-01-01

    Full Text Available fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4 and Zhongwan (CV12 were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation.

  3. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    The effect of sodium cyanide on the morphology of stem, leaves and grain yields of Triticum durum cv. Altar and Triticum aestivum cv. Cumhuriyet grown under glass was studied. Seeds were planted in six different sets of pots containing ordinary garden soil. After formation of the first leaves......, the first set was used as the control and watered using ordinary bottled water sold commercially. The other five sets with T. durum cv. Altar and T. aestivum cv. Cumhuriyet seedlings were additionally watered with various concentrations of sodium cyanide, the test-quantity used being 10-50 mg/L. Growth...... of individual plants was monitored until grain production. It was found that the sodium cyanide concentrations in the feed solutions affected plant stature, with the plants becoming progressively dwarfed with increasing dosage. Anomalies in the morphological and anatomical structure of the plant were also noted...

  4. Prediction of ROSA-III experiment Run 702

    International Nuclear Information System (INIS)

    Koizumi, Yasuo; Soda, Kunihisa; Kikuchi, Osamu.

    1978-11-01

    The purpose of the ROSA-III experiment with a scaled BWR test facility is to examine primary coolant thermalhydraulic behavior and performance during a postulated loss-of-coolant accident of BWR. The results provide information for verification and improvement of reactor safety analysis codes. Run 702 assumes a recirculation line double ended break at the pump suction with average core power and no ECCS. Prediction of the Run 702 experiment was made with computer code RELAP-4J. What determine the coolant behavior are mixture level in the downcomer and flowrates and flow directions at jet pump drive flow nozzle, jet pump suction and discharge. There is thus the need for these measurements to compare predicted results with experimental ones. The liquid level formation model also needs improvement. (author)

  5. Experiment data of ROSA-III integral test RUN 710

    International Nuclear Information System (INIS)

    Koizumi, Yasuo; Tasaka, Kanji; Adachi, Hiromichi; Anoda, Yoshinari; Soda, Kunihisa

    1981-01-01

    The report presents data of RUN 710 at ROSA-III test facility. RUN 710 simulates a 200% double-ended break at the inlest side of a recirculation pump of a BWR. All ECCS are activated and electric power to simulated fuel rods in one core channel among four is not supplied in RUN 710. The primary initial conditions are steam dome pressure 7.35 MPa, lower plenum subcooling 10.8 K, core inlet flow rate 31.3 kg/s and core heat generation 2.42 MW. Peak cladding temperature is 609 K at Position 3, 352.5 mm above the mid plane of the core. All heater rods are quenched after ECCS actuation and the effectiveness of ECCS is confirmed. (author)

  6. [Structural organization of 5S ribosomal DNA of Rosa rugosa].

    Science.gov (United States)

    Tynkevych, Iu O; Volkov, R A

    2014-01-01

    In order to clarify molecular organization of the genomic region encoding 5S rRNA in diploid species Rosa rugosa several 5S rDNA repeated units were cloned and sequenced. Analysis of the obtained sequences revealed that only one length variant of 5S rDNA repeated units, which contains intact promoter elements in the intergenic spacer region (IGS) and appears to be transcriptionally active is present in the genome. Additionally, a limited number of 5S rDNA pseudogenes lacking a portion of coding sequence and the complete IGS was detected. A high level of sequence similarity (from 93.7 to 97.5%) between the IGS of major 5S rDNA variants of East Asian R. rugosa and North American R. nitida was found indicating comparatively recent divergence of these species.

  7. Socialising Semiotic Mediation: Some Reflections on Rosa and Pievi's Paper

    DEFF Research Database (Denmark)

    Glaveanu, Vlad Petre

    2013-01-01

    on similarities but also addresses and exploits several important conceptual differences. The article proceeds by outlining the areas in which SRT can be enriched by a semiotic account – such as incorporating a more clearly articulated theory of signs and unpacking further the role of mediated action and personal......This article aims to reflect on the relation between social representations theory (SRT) and a cultural psychological view of semiotics, as presented in Rosa and Pievi's paper (this issue). It is argued that a fruitful dialogue can be established between the two orientations, one that draws...... experience – as well as the ways in which the latter can benefit from engaging with the notion of social representations. Most of all, I propose that what a SRT-informed view of cultural psychology can offer us is a ‘socialized model of semiotic mediation', one that grounds the construction of knowledge...

  8. Rose hip (Rosa canina L: A functional food perspective

    Directory of Open Access Journals (Sweden)

    Cui Fan

    2014-12-01

    Full Text Available Rose hip (Rosa canina L. is the pseudo-fruit of the rose plant, which is widely known as a valuable source of polyphenols and vitamin C. Both in vivo and in vitro studies have demonstrated that this fruit exhibits anti-inflammatory, antioxidant, and antiobesogenic activities. The health benefits of Rose hip (RH have been attributed to its wide range of bioactive compounds including the anti-inflammatory galactolipid: (2S-1,2-di-O-[(9Z,12Z,15Z-octadeca-9,12,15-trienoyl]-3-O-β-D galactopyranosyl glycerol (GOPO, vitamin C, phenolics, lycopene, lutein, zeaxanthin, and other carotenoids. As cyclooxygenase inhibitors, RH compounds may reduce the risk of cancer, heart disease, and various inflammatory conditions. The aim of this review is to present an overview of the functional, medical, and physiological properties of RH.

  9. The Dmp1-SOST Transgene Interacts With and Downregulates the Dmp1-Cre Transgene and the Rosa(Notch) Allele.

    Science.gov (United States)

    Zanotti, Stefano; Canalis, Ernesto

    2016-05-01

    Activation of Notch1 in osteocytes of Rosa(Notch) mice, where a loxP-flanked STOP cassette and the Nicd coding sequence were targeted to the reverse orientation splice acceptor (Rosa)26 locus, causes osteopetrosis associated with suppressed Sost expression and enhanced Wnt signaling. To determine whether Sost downregulation mediates the effects of Notch activation in osteocytes, Rosa(Notch) mice were crossed with transgenics expressing Cre recombinase or SOST under the control of the dentin matrix protein (Dmp)1 promoter. Dmp1-SOST transgenics displayed vertebral osteopenia and a modest femoral cancellous and cortical bone phenotype, whereas hemizygous Dmp1-Cre transgenics heterozygous for the Rosa(Notch) allele (Dmp1-Cre;Rosa(Notch)) exhibited osteopetrosis. The phenotype of Notch activation in osteocytes was prevented in Dmp1-Cre;Rosa(Notch) mice hemizygous for the Dmp1-SOST transgene. The effect was associated with downregulated Notch signaling and suppressed Dmp1 and Rosa26 expression. To test whether SOST regulates Notch expression in osteocytes, cortical bone cultures from Dmp1-Cre;Rosa(Notch) mice or from Rosa(Notch) control littermates were exposed to recombinant human SOST. The addition of SOST had only modest effects on Notch target gene mRNA levels and suppressed Dmp1, but not Cre or Rosa26, expression. These findings suggest that prevention of the Dmp1-Cre;Rosa(Notch) skeletal phenotype by Dmp1-SOST is not secondary to SOST expression but to interactions among the Dmp1-SOST and Dmp1-Cre transgenes and the Rosa26 locus. In conclusion, the Dmp1-SOST transgene suppresses the expression of the Dmp1-Cre transgene and of Rosa26. © 2015 Wiley Periodicals, Inc.

  10. Nitrogen remobilisation facilitates adventitious root formation on reversible dark-induced carbohydrate depletion in Petunia hybrida.

    Science.gov (United States)

    Zerche, Siegfried; Haensch, Klaus-Thomas; Druege, Uwe; Hajirezaei, Mohammad-Reza

    2016-10-10

    Adventitious root (AR) formation in axillary shoot tip cuttings is a crucial physiological process for ornamental propagation that is utilised in global production chains for young plants. In this process, the nitrogen and carbohydrate metabolisms of a cutting are regulated by its total nitrogen content (N t ), dark exposure during transport and irradiance levels at distinct production sites and phases through a specific plasticity to readjust metabolite pools. Here, we examined how elevated N t contents with a combined dark exposure of cuttings influence their internal N-pools including free amino acids and considered early anatomic events of AR formation as well as further root development in Petunia hybrida cuttings. Enhanced N t contents of unrooted cuttings resulted in elevated total free amino acid levels and in particular glutamate (glu) and glutamine (gln) in leaf and basal stem. N-allocation to mobile N-pools increased whereas the allocation to insoluble protein-N declined. A dark exposure of cuttings conserved initial N t and nitrate-N, while it reduced insoluble protein-N and increased soluble protein, amino- and amide-N. The increase of amino acids mainly comprised asparagine (asn), aspartate (asp) and arginine (arg) in the leaves, with distinct tissue specific responses to an elevated N supply. Dark exposure induced an early transient rise of asp followed by a temporary increase of glu. A strong positive N effect of high N t contents of cuttings on AR formation after 384 h was observed. Root meristematic cells developed at 72 h with a negligible difference for two N t levels. After 168 h, an enhanced N t accelerated AR formation and gave rise to first obvious fully developed roots while only meristems were formed with a low N t . However, dark exposure for 168 h promoted AR formation particularly in cuttings with a low N t to such an extent so that the benefit of the enhanced N t was almost compensated. Combined dark exposure and low N t of

  11. CRED REA Algal Assessment, Santa Rosa Bank 2003 (NODC Accession 0010352)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Twelve quadrats were sampled along 2 consecutively-placed, 25m transect lines as part of Rapid Ecological Assessments conducted at 2 sites at Santa Rosa Bank (off...

  12. Breeding of Hibiscus rosa-sinensis for garden use in Denmark

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna; Simonsen, Maria Boye; Lütken, Henrik Vlk

    2013-01-01

    -sinensis for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa......Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa......-sinensis suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities...

  13. High-Performance Elastically Self-Deployed Roll-Out Solar Array (ROSA), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Deployable Space Systems (DSS) has developed an ultra-lightweight elastically self-deployable roll-out solar array (ROSA) structural platform that when combined with...

  14. AFLP Markers as a tool to reconstruct complex relationships: a case study in Rosa (Rosaceae)

    NARCIS (Netherlands)

    Koopman, W.J.M.; Wissemann, V.; Cock, de K.; Huylenbroeck, van J.; Riek, de J.; Sabatino, G.J.H.; Visser, D.L.; Vosman, B.; Ritz, K.; Maes, B.; Werlemark, G.; Nybom, H.; Debener, T.; Linde, M.; Smulders, M.J.M.

    2008-01-01

    The genus Rosa has a complex evolutionary history caused by several factors, often in conjunction: extensive hybridization, recent radiation, incomplete lineage sorting, and multiple events of polyploidy. We examined the applicability of AFLP markers for reconstructing (species) relationships in

  15. The metabolism of sorbitol and fructose in isolated chloroplasts of Santa Rosa plum leaves

    International Nuclear Information System (INIS)

    De Villiers, O.T.

    1979-01-01

    Aqueously as well as non-aqueously isolated chloroplasts from Santa Rosa plum leaves readily metabolised sorbitol- 14 C to fructose, glucose and sucrose. Likewise, fructose- 14 C was converted to sorbitol, glucose and sucrose [af

  16. ROSA P : The National Transportation Library’s Repository and Open Science Access Portal

    Science.gov (United States)

    2018-01-01

    The National Transportation Library (NTL) was founded as an all-digital repository of US DOT research reports, technical publications and data products. NTLs primary public offering is ROSA P, the Repository and Open Science Access Portal. An open...

  17. CV controls from design to operation

    CERN Document Server

    Blanc, D

    2002-01-01

    The cooling and Ventilation (CV) group has emphasised the need to redefine its organisational structure at the end of 98. The main objective of this operation was to ensure the CV group to be more competitive and efficient through the growing tasks of the LHC projects. The main evolution given to this reorganisation is that the new structure is more project oriented and then operates on three distinct axes: Design, Work and Operation. Process control project management requires a complete and early interaction and participation of all the actors involved. This procedure to be efficient and constructive must be considered and performed not only during the design stage but along the project planning phases and must go beyond the completion work including the process control operation activity. The paper explains the present project management for process control. It describes the present constraints and gives suggestions to a different approach to these projects to improve performances and efficiency of a contr...

  18. Grande Sertão: Veredas by João Guimarães Rosa

    Directory of Open Access Journals (Sweden)

    2013-07-01

    Full Text Available "What is a translation that stalls comprehension?" asks Nancy Fumero in her introduction to Felipe W. Martinez' English-language renditions of João Guimarães Rosa's Portuguese poetics. In these translations (presented alongside the Portuguese originals Fumero argues that we encounter "a new form of translation that risks everything in order to encounter the same treacherous knowing Rosa had traversed."

  19. EARTH, MOON, SUN, AND CV ACCRETION DISKS

    International Nuclear Information System (INIS)

    Montgomery, M. M.

    2009-01-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if

  20. The effect of fertilizer level and foliar-applied calcium on seed production and germination of Gerbera hybrida

    DEFF Research Database (Denmark)

    Andreasen, Christian; Kemezys, Andrius Hansen; Müller, Renate

    2014-01-01

    an additional foliar calcium application influenced the same parameters. Subsequently, the effect of the various treatments on the germination of the obtained seeds was explored. Two identical experiments (A and B) were carried out with five concentrations of nutrient solutions corresponding to an electrical...... and seed number, but seed weight and plant biomass were significantly reduced at the highest fertilizer concentration. In both experiments, the seeds germinated slower and less seeds germinated when plants had received the largest amount of fertilizer (6.25 mS·cm-1). In none of the experiments did applied......Gerbera hybrida is an ornamental plant of great commercial interest, which is primarily propagated by seeds. We investigated whether increasing fertilizer concentrations during seed set enhanced plant biomass, number of flower heads, seed set, and seed weight. Furthermore, we studied whether...

  1. Learning openCV computer vision with the openCV library

    CERN Document Server

    Bradski, Gary

    2008-01-01

    Learning OpenCV puts you right in the middle of the rapidly expanding field of computer vision. Written by the creators of OpenCV, the widely used free open-source library, this book introduces you to computer vision and demonstrates how you can quickly build applications that enable computers to see" and make decisions based on the data. With this book, any developer or hobbyist can get up and running with the framework quickly, whether it's to build simple or sophisticated vision applications

  2. Reproductive success of Whiskered Tern Chlidonias hybrida in eastern Spain in relation to water level variation.

    Science.gov (United States)

    Ortiz Lledó, Álvaro; Vidal Mateo, Javier; Urios Moliner, Vicente

    2018-01-01

    A study on the Whiskered Tern Chlidonias hybrida was carried out between 2002 and 2009 in wetlands of eastern Spain to evaluate how water level fluctuation affects its reproductive success (hatching, fledgling and breeding success). This species is catalogued as Vulnerable in Spain and has an unfavorable conservation status in Europe. Our study includes 18 sampling areas from five wetlands, covering a total of 663 nests, 1,618 eggs, 777 nestlings and 225 fledglings. The colonies were visited at least twice per week in breeding period. The number of eggs and/or nestlings present in each nest were annotated each time the colonies were visited with the aim to compare the evolution of these parameters with time. Hatching success was calculated as the proportion of egg that hatched successfully. Fledgling success and breeding success were calculated as the proportion of chicks that fledged successfully and the proportion of eggs that produced fledglings. We used the Kruskal-Wallis test to analyze the differences in the dependent variables hatching, fledgling and breeding success among the wetlands and the sampling areas. We explored the relationship between the different reproductive success with the average fluctuation rate and the anchoring depth of nests, using statistics of the linear regression. It was observed that the reproductive success varied significantly in the interaction among the different categories of water level fluctuation and the different areas (using the Kruskal-Wallis test). Our records showed that pronounced variations in water level destroyed several nests, which affected the Whiskered Tern reproductive success. Considering all events that occurred in 18 areas, the mean (±SD) of nests, eggs and nestlings that were lost after water level fluctuations were of 25.60 ± 21.79%, 32.06 ± 27.58% and 31.91 ± 21.28% respectively, also including the effects of rain and predation. Unfavorable climatic events, such as strong wind, rain or hail, also

  3. Reproductive success of Whiskered Tern Chlidonias hybrida in eastern Spain in relation to water level variation

    Directory of Open Access Journals (Sweden)

    Álvaro Ortiz Lledó

    2018-04-01

    Full Text Available Background A study on the Whiskered Tern Chlidonias hybrida was carried out between 2002 and 2009 in wetlands of eastern Spain to evaluate how water level fluctuation affects its reproductive success (hatching, fledgling and breeding success. This species is catalogued as Vulnerable in Spain and has an unfavorable conservation status in Europe. Methods Our study includes 18 sampling areas from five wetlands, covering a total of 663 nests, 1,618 eggs, 777 nestlings and 225 fledglings. The colonies were visited at least twice per week in breeding period. The number of eggs and/or nestlings present in each nest were annotated each time the colonies were visited with the aim to compare the evolution of these parameters with time. Hatching success was calculated as the proportion of egg that hatched successfully. Fledgling success and breeding success were calculated as the proportion of chicks that fledged successfully and the proportion of eggs that produced fledglings. We used the Kruskal–Wallis test to analyze the differences in the dependent variables hatching, fledgling and breeding success among the wetlands and the sampling areas. We explored the relationship between the different reproductive success with the average fluctuation rate and the anchoring depth of nests, using statistics of the linear regression. Results It was observed that the reproductive success varied significantly in the interaction among the different categories of water level fluctuation and the different areas (using the Kruskal–Wallis test. Our records showed that pronounced variations in water level destroyed several nests, which affected the Whiskered Tern reproductive success. Considering all events that occurred in 18 areas, the mean (±SD of nests, eggs and nestlings that were lost after water level fluctuations were of 25.60 ± 21.79%, 32.06 ± 27.58% and 31.91 ± 21.28% respectively, also including the effects of rain and predation. Discussion Unfavorable

  4. Effect of Mentha pulegium extract and 8-hydroxy quinoline sulphate to extend the quality and vase life of rose (Rosa hybrid) cut flower.

    Science.gov (United States)

    Hashemabadi, Davood; Torkashvand, Ali Mohammadi; Kaviani, Behzad; Bagherzadeh, Maryam; Rezaalipour, Mohaddeseh; Zarchini, Mohammad

    2015-01-01

    Rose is an ornamental plant which contains one of the world's top cut flowers. Vase life of cut rose flower is short. Extracts of Mentha pulegium and 8-hydroxy quinoline sulphate (8-HQS) were used as two preservative solutions, aiming to extend the vase life of cut rose (Rosa hybrid L.) flowers. Rose flowers were treated with a vase solution containing the extract of M. pulegium, at concentrations of 0, 10, 20 and 30%, in combination with 8-HQS at concentrations of 0, 200, 400 and 600 mg l(-1). Longevity of cut roses flowers was determined on the basis of wilting and chlorophyll retention. Cut roses flowers were kept at room temperature (20 ± 2 degrees C) under normal day light and natural ventilation. The vase life of cut flowers studied was prolonged by all 8-HQS and extract treatments. The best concentration of 8-HQS and extractwere 400 mg l(-1) and 10%, respectively. Our results indicated that the flowers treated with the extract and 8-HQS had longer vase life, higher rate of solution uptake and lower SPAD value (total chlorophyll) compared to the control. Also, cut flowers treated with the extract and 8-HQS had least bacterial colonies. The greatest longevity of vase life by 11.20 and 10.25 days was related to 400 mg I(-1) 8-HQS and 10% of extract, respectively. These treatments improved cut vase life more than the control treatment. The maximum solution uptake (1.85 ml g(-1) f.wt.) and minimum SPAD value (2.19) were calculated in 30% extract along with 200 mg l(-1) 8-HQS, and 200 mg l(-1) 8-HQS, respectively. The lowest number of bacterial colonies (55.75) was obtained in treatment of 600 mg l(-1) 8-HQS. Flower quality of specimens treated with extract and 8-HQS was better than those of the control. The experiments were repeated three times with three replicates and a completely randomized design had been used. The present study concludes that it would be possible to use preservative solutions containing extract of M. pulegium L. and 8-HQS to extend vase

  5. Hydrologic and geochemical characterization of the Santa Rosa Plain watershed, Sonoma County, California

    Science.gov (United States)

    Nishikawa, Tracy

    2013-01-01

    The Santa Rosa Plain is home to approximately half of the population of Sonoma County, California, and faces growth in population and demand for water. Water managers are confronted with the challenge of meeting the increasing water demand with a combination of water sources, including local groundwater, whose future availability could be uncertain. To meet this challenge, water managers are seeking to acquire the knowledge and tools needed to understand the likely effects of future groundwater development in the Santa Rosa Plain and to identify efficient strategies for surface- and groundwater management that will ensure the long-term viability of the water supply. The U.S. Geological Survey, in cooperation with the Sonoma County Water Agency and other stakeholders in the area (cities of Cotati, Rohnert Park, Santa Rosa, and Sebastopol, town of Windsor, Cal-American Water Company, and the County of Sonoma), undertook this study to characterize the hydrology of the Santa Rosa Plain and to develop tools to better understand and manage the groundwater system. The objectives of the study are: (1) to develop an updated assessment of the hydrogeology and geochemistry of the Santa Rosa Plain; (2) to develop a fully coupled surface-water and groundwater-flow model for the Santa Rosa Plain watershed; and (3) to evaluate the potential hydrologic effects of alternative groundwater-management strategies for the basin. The purpose of this report is to describe the surface-water and groundwater hydrology, hydrogeology, and water-quality characteristics of the Santa Rosa Plain watershed and to develop a conceptual model of the hydrologic system in support of the first objective. The results from completing the second and third objectives will be described in a separate report.

  6. Organisation des travaux ST-CV

    CERN Document Server

    Martel, C; CERN. Geneva. ST Division

    2003-01-01

    Débutée il y a deux ans, la phase de construction des installations de refroidissement du LHC a atteint en 2003 son apogée. De nombreux autres projets sont également gérés par le groupe dans divers secteurs du laboratoire. La spécificité du groupe est de réaliser des installations complexes impliquant des spécificités diverses telles qu’aéraulique, hydraulique, électrique, etc. Pour faire face à ces demandes la section travaux du groupe ST CV a adopté une structure capable de réaliser ces divers projets avec un minimum de personnel. La première partie de ce document décrit l’organisation de la section travaux ST CV. Dans une deuxième partie, les auteurs dressent un état des lieux des chantiers par projet en décrivant plus particulièrement les activités dans les domaines du traitement d’air et de refroidissement par eau.

  7. Retendering of ST-CV maintenance contract

    CERN Document Server

    Battistin, M

    2003-01-01

    During the next three years, the ST/CV group will be deeply involved in the installation works for the LHC project. During this period the need for maintenance activity will decrease. The minimum level will be reached during the “long shutdown” of the PS and SPS machines in 2005. The budget for the maintenance will decrease accordingly, thus the CV group had to review its maintenance strategy. The new contract, which started on January 1st 2003, has been defined to cope with these workload variations during the next years and to guarantee the minimum maintenance activity on the existing equipment. A lump-sum contract based on a win-win strategy has been discarded. The contractor no longer has to guarantee the performance of the CERN cooling and ventilation systems. A new price list strategy based on performance indicators and penalties has been chosen. The contractor now has to guarantee the performance of every maintenance operation demanded by CERN. This modification obliged the Operation section of the...

  8. Fullerene-Based Symmetry in Hibiscus rosa-sinensis Pollen

    Science.gov (United States)

    Andrade, Kleber; Guerra, Sara; Debut, Alexis

    2014-01-01

    The fullerene molecule belongs to the so-called super materials. The compound is interesting due to its spherical configuration where atoms occupy positions forming a mechanically stable structure. We first demonstrate that pollen of Hibiscus rosa-sinensis has a strong symmetry regarding the distribution of its spines over the spherical grain. These spines form spherical hexagons and pentagons. The distance between atoms in fullerene is explained applying principles of flat, spherical, and spatial geometry, based on Euclid’s “Elements” book, as well as logic algorithms. Measurements of the pollen grain take into account that the true spine lengths, and consequently the real distances between them, are measured to the periphery of each grain. Algorithms are developed to recover the spatial effects lost in 2D photos. There is a clear correspondence between the position of atoms in the fullerene molecule and the position of spines in the pollen grain. In the fullerene the separation gives the idea of equal length bonds which implies perfectly distributed electron clouds while in the pollen grain we suggest that the spines being equally spaced carry an electrical charge originating in forces involved in the pollination process. PMID:25003375

  9. ROSA-III 50 % break integral test RUN 916

    International Nuclear Information System (INIS)

    Yonomoto, Taisuke; Tasaka, Kanji; Koizumi, Yasuo; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Suzuki, Mitsuhiro; Murata, Hideo

    1985-08-01

    This report presents the experimental data of RUN 916 conducted at the ROSA-III test facility. The facility is a volumetrically scaled (1/424) simulator for a BWR/6 with the electrically heated core, the break simulator and the scaled ECCS(emergency core cooling system). RUN 916 was a 50 % split break test at the recirculation pump suction line with an assumption of HPCS diegel generator failure and conducted as one of the break area parameter tests. A peak cladding temperature (PCT) of 917 K was reached at 190 s after the break during the reflooding phase. Whole core was completely quenched by ECCS, and the effectiveness of ECCS was confermed. The primary test results of RUN 916 are compared in this report with those of RUN 926, which was a 200 % double-ended break test. The initiation of core dryout in RUN 916 was later than that in RUN 926 because of the smaller discharge flow rate. Duration of core dryourt was, however, longer in RUN 916 because of later actuation of ECCSs. PCT in RUN 916 was 133 K higher than that in RUN 926. (author)

  10. ROSA-III 100 % break integral test Run 914

    International Nuclear Information System (INIS)

    Yonomoto, Taisuke; Tasaka, Kanji; Koizumi, Yasuo; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Suzuki, Mitsuhiro; Murata, Hideo

    1987-05-01

    This report presents the experimental data of RUN 914 conducted at the ROSA-III test facility. The facility is a volumetrically scaled (1/424) simulator for a BWR/6 with the electrically heated core, the break simulator and the scaled ECCS (emergency core cooling system). RUN 914 was a 100% split break test at the recirculation pump suction line with an assumption of HPCS diesel generator failure and conducted as one of the break area parameter tests. A peak cladding temperature (PCT) of 851 K was reached at 130 s after the break during the reflooding phase. Whole core was completely quenched by ECCS, and the effectiveness of ECCS was confirmed. The primary test results of RUN 914 are compared in this report with those of RUN 926, which was a 200 % double-ended break test. The initiation of core dryout in RUN 914 was almost the same as that in RUN 926. Duration of core dryourt was, however, longer in RUN 914 because of later actuation of ECCSs. PCT in RUN 914 was 67 K higher than that in RUN 926. (author)

  11. Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation.

    OpenAIRE

    Ahkami, Amir H.; Melzer, Michael; Ghaffari, Mohammad R.; Pollmann, Stephan; Ghorbani, Majid; Shahinnia, Fahimeh; Hajirezaei, Mohammad R.; Druege, Uwe

    2013-01-01

    To determine the contribution of polar auxin transport (PAT) to auxin accumulation and to adventitious root (AR) formation in the stem base of Petunia hybrida shoot tip cuttings, the level of indole-3-acetic acid (IAA) was monitored in non-treated cuttings and cuttings treated with the auxin transport blocker naphthylphthalamic acid (NPA) and was complemented with precise anatomical studies. The temporal course of carbohydrates, amino acids and activities of controlling enzymes was also inves...

  12. Expression of transgenes targeted to the Gt(ROSA26Sor locus is orientation dependent.

    Directory of Open Access Journals (Sweden)

    Douglas Strathdee

    2006-12-01

    Full Text Available Targeting transgenes to a chosen location in the genome has a number of advantages. A single copy of the DNA construct can be inserted by targeting into regions of chromatin that allow the desired developmental and tissue-specific expression of the transgene.In order to develop a reliable system for reproducibly expressing transgenes it was decided to insert constructs at the Gt(ROSA26Sor locus. A cytomegalovirus (CMV promoter was used to drive expression of the Tetracycline (tet transcriptional activator, rtTA2(s-M2, and test the effectiveness of using the ROSA26 locus to allow transgene expression. The tet operator construct was inserted into one allele of ROSA26 and a tet responder construct controlling expression of EGFP was inserted into the other allele.Expression of the targeted transgenes was shown to be affected by both the presence of selectable marker cassettes and by the orientation of the transgenes with respect to the endogenous ROSA26 promoter. These results suggest that transcriptional interference from the endogenous gene promoter or from promoters in the selectable marker cassettes may be affecting transgene expression at the locus. Additionally we have been able to determine the optimal orientation for transgene expression at the ROSA26 locus.

  13. Identification of two new races of Diplocarpon rosae Wolf, the causal agent of rose black spot disease

    Science.gov (United States)

    The fungal pathogen, Diplocarpon rosae Wolf, infects only roses (Rosa spp.) and leads to rose black spot disease. Rose black spot is the most problematic disease of outdoor grown roses worldwide, due to the potential for rapid leaf yellowing and defoliation. Plants repeatedly defoliated from black ...

  14. 77 FR 37318 - Eighth Coast Guard District Annual Safety Zones; Sound of Independence; Santa Rosa Sound; Fort...

    Science.gov (United States)

    2012-06-21

    ...-AA00 Eighth Coast Guard District Annual Safety Zones; Sound of Independence; Santa Rosa Sound; Fort... Coast Guard will enforce a Safety Zone for the Sound of Independence event in the Santa Rosa Sound, Fort... during the Sound of Independence. During the enforcement period, entry into, transiting or anchoring in...

  15. Variety of plant pathogens of ornamental shrubs of the genus Rosa L.

    Directory of Open Access Journals (Sweden)

    Marchenko Alla Borisovna

    2015-12-01

    In the Ukraine’s forest-steppe environment, over the years of research, the most common disease among different representatives of the genus Rosa L. is Diplocarpon rosae FA Wolf, about 52.7%. Diseases caused by Sphaerotheca pannosa var. rosae Woron account for 28.3%: Coniothyrium wernsdorffiae Laubert―19.2%; Botrytis cinerea Pers.―16.9%; Phragmidium mucronatum (Pers. Schltdl., Phragmidium tuberculatum Müll. Hal., Nectria cinnabarina (Tode Fr.―from 7.2 to 6.7%. Pathogens Alternaria alternata (Fr. Keissl., Alternaria tenuissima (Kunze Wiltshire, Fusarium oxysporum Schltdl., Fusarium solani (Mart. Sacc., Phytophthora cactorum J. Schröt., Verticillium dahliae Kleb., Verticillium albo-atrum Reinke Berthold are less common―5%.

  16. Effect of Rosa damascene aromatherapy on sleep quality in cardiac patients: a randomized controlled trial.

    Science.gov (United States)

    Hajibagheri, Ali; Babaii, Atye; Adib-Hajbaghery, Mohsen

    2014-08-01

    Sleep disorders are common among patients hospitalized in coronary care unit (CCU). This study aimed to investigate the effect of Rosa damascene aromatherapy on sleep quality of patients hospitalized in CCU. In this randomized controlled trial, 60 patients who met the inclusion criteria were conveniently sampled and randomly allocated to the experimental and control groups. Patients in the control group received routine care. In the experimental group, patients received routine care and Rosa damascene aromatherapy for three subsequent nights. In the both groups the sleep quality was assessed using the Pittsburgh Sleep Quality Index. After the study, the mean scores of five domains of Pittsburg Sleep Quality Index as well as the mean of total score of the index in the experimental group were significantly lower than the control group. Rosa damascene aromatherapy can significantly improve the sleep quality of patients hospitalized in CCUs. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii.

    Science.gov (United States)

    Kumar, Neeraj; Bhandari, Pamita; Singh, Bikram; Bari, Shamsher S

    2009-02-01

    Roses are one of the most important groups of ornamental plants and their fruits and flowers are used in a wide variety of food, nutritional products and different traditional medicines. The antioxidant activity of methanolic extracts from fresh flowers of three rose species (Rosa damascena, Rosa bourboniana and Rosa brunonii) was evaluated by 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical method. The ability to scavenge DPPH radical was measured by the discoloration of the solution. The methanolic extract from R. brunonii exhibited maximum free-radical-scavenging activity (64.5+/-0.38%) followed by R. bourboniana (51.8+/-0.46%) and R. damascena (43.6+/-0.25%) at 100 microg/ml. Simultaneously, ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study phenolic composition in the methanolic extracts from the fresh flowers of rose species. The phenolic constituents were further investigated by direct infusion-ESI-QTOF-MS/MS in negative ion mode. Characteristic Electrospray ionization tandem mass spectrometry (ESI-MS/MS) spectra with other diagnostic fragment ions generated by retro Diels-Alder (RDA) fragmentation pathways were recorded for the flavonoids. Distinct similarities were observed in the relative distribution of polyphenolic compounds among the three species. The dominance of quercetin, kaempferol and their glycosides was observed in all the three species.

  18. Petra : la ciudad rosa del desierto (Historia y urbanismo

    Directory of Open Access Journals (Sweden)

    Ana María Vázquez Hoys

    1997-01-01

    Full Text Available Petra, la ciudad rosa del desierto jordano, es conocida, sobre todo, por el bello color de sus piedras. A menudo se olvida su importancia histórica, como sede de los edomitas, enemigos de Israel en el Antiguo Testamento y más tarde de los nabateos, pueblo nómada árabe de excelentes comerciantes, intermediarios entre el Próximo Oriente y Occidente. Sólo el pueblo romano, en el siglo ii d. C. cambió la suerte de Petra, que permaneció olvidada durante diez y siete siglos. En los últimos tiempos, el esfuerzo de los viajeros y arqueólogos ha permitido conocer quienes eran sus habitantes, donde vivían, qué pensaban y cuales eran sus dioses.Petra, the «pink town» of the Jordán desert, is mainly known for its beautifully colored stones. Often, we may forget about its historical significance as headquarters of the edomites —Israel's enemies in the Oíd Testament— and, aftenvards, royal city of the nabateans, Arabian nomads mainly known for their comercial importance as intermediarles between the near East and Occident. It was only on the II century after J.C. that the Román people changed Petra's chance, letting the city remain «forgotten» during almost seventeen centuries. On the last two centuries, Petra was awakened of its lethargic sieep by unfatigable travelers and archaeclogists. Thanks to them, we have got to know about Petra's inhabitants, their thoughts, their ways of thinking, how they were living, and the ñame of their gods.

  19. IJ-OpenCV: Combining ImageJ and OpenCV for processing images in biomedicine.

    Science.gov (United States)

    Domínguez, César; Heras, Jónathan; Pascual, Vico

    2017-05-01

    The effective processing of biomedical images usually requires the interoperability of diverse software tools that have different aims but are complementary. The goal of this work is to develop a bridge to connect two of those tools: ImageJ, a program for image analysis in life sciences, and OpenCV, a computer vision and machine learning library. Based on a thorough analysis of ImageJ and OpenCV, we detected the features of these systems that could be enhanced, and developed a library to combine both tools, taking advantage of the strengths of each system. The library was implemented on top of the SciJava converter framework. We also provide a methodology to use this library. We have developed the publicly available library IJ-OpenCV that can be employed to create applications combining features from both ImageJ and OpenCV. From the perspective of ImageJ developers, they can use IJ-OpenCV to easily create plugins that use any functionality provided by the OpenCV library and explore different alternatives. From the perspective of OpenCV developers, this library provides a link to the ImageJ graphical user interface and all its features to handle regions of interest. The IJ-OpenCV library bridges the gap between ImageJ and OpenCV, allowing the connection and the cooperation of these two systems. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. The duplicated B-class heterodimer model: whorl-specific effects and complex genetic interactions in Petunia hybrida flower development.

    Science.gov (United States)

    Vandenbussche, Michiel; Zethof, Jan; Royaert, Stefan; Weterings, Koen; Gerats, Tom

    2004-03-01

    In both Antirrhinum (Antirrhinum majus) and Arabidopsis (Arabidopsis thaliana), the floral B-function, which specifies petal and stamen development, is embedded in a heterodimer consisting of one DEFICIENS (DEF)/APETALA3 (AP3)-like and one GLOBOSA (GLO)/PISTILLATA (PI)-like MADS box protein. Here, we demonstrate that gene duplications in both the DEF/AP3 and GLO/PI lineages in Petunia hybrida (petunia) have led to a functional diversification of their respective members, which is reflected by partner specificity and whorl-specific functions among these proteins. Previously, it has been shown that mutations in PhDEF (formerly known as GREEN PETALS) only affect petal development. We have isolated insertion alleles for PhGLO1 (FLORAL BINDING PROTEIN1) and PhGLO2 (PETUNIA MADS BOX GENE2) and demonstrate unique and redundant properties of PhDEF, PhGLO1, and PhGLO2. Besides a full homeotic conversion of petals to sepals and of stamens to carpels as observed in phglo1 phglo2 and phdef phglo2 flowers, we found that gene dosage effects for several mutant combinations cause qualitative and quantitative changes in whorl 2 and 3 meristem fate, and we show that the PHDEF/PHGLO1 heterodimer controls the fusion of the stamen filaments with the petal tube. Nevertheless, when the activity of PhDEF, PhGLO1, and PhGLO2 are considered jointly, they basically appear to function as DEF/GLO does in Antirrhinum and to a lesser extent as AP3/PI in Arabidopsis. By contrast, our data suggest that the function of the fourth B-class MADS box member, the paleoAP3-type PETUNIA HYBRIDA TM6 (PhTM6) gene, differs significantly from the known euAP3-type DEF/AP3-like proteins; PhTM6 is mainly expressed in the developing stamens and ovary of wild-type flowers, whereas its expression level is upregulated in whorls 1 and 2 of an A-function floral mutant; PhTM6 is most likely not involved in petal development. The latter is consistent with the hypothesis that the evolutionary origin of the higher eudicot

  1. El ritmo de intensidad en La rosa escrita de Xavier Abril

    Directory of Open Access Journals (Sweden)

    Óscar Coello

    2011-07-01

    Full Text Available Luego de las aventuras rítmicas practicadas por Abril en las más audaces posiciones de vanguardia, se produce una abjuración insólita –explicada luego metatextualmente– en la poiesis del artista, que lo regresa a los cauces de la elaboración versal tradicional. La rosa escrita es el poemario más emblemático de este retorno. La presente ponencia ejemplifica en un texto, a través del ritmo de intensidad, los pormenores de esta redención.Palabras claves: Xavier Abril, La rosa escrita, Vanguardia, ritmo.

  2. MORPHOLOGICAL AND MICROMORPHOLOGICAL INVESTIGATIONS REGARDING THE LEAVES OF SEVERAL ROSA L. SPECIES

    Directory of Open Access Journals (Sweden)

    Lidia ADUMITRESEI

    2016-12-01

    Full Text Available Multicellular secretory glands are present on Rosa L. species, at least on the stipellae edge; some of them have a larger number and different particularities. The researches have been made on leaves of the following species: Rosa agrestis, R. x damascena, R. multibracteata, R. pimpinellifolia and R. rubiginosa. Micromorphological studies emphasized a large number of cells which forming both terminal secretory part and foot, even though their dimensions are small. These researches highlight the micromorphological aspect of these glands, tector hairs and epicuticular wax. All of them were examined through scanning electron microscopy method.

  3. Exploitation of the bloc 17: development schemes for Rosa-Lirio; L'exploitation du bloc 17: des schemas de developpement pour Rosa-Lirio

    Energy Technology Data Exchange (ETDEWEB)

    Legros, E.

    2002-12-01

    New developments are expected on the prolific bloc 17 of the Angola offshore. Among the tenth of discoveries that followed the discovery of the Girassol giant field, TotalFinaElf initially selected the Dalia field, for which engineering studies started in December 2000. However, it is still not excluded that the development of the Rosa-Lirio field will start first. This article describes the specific characteristics of this field and the solutions proposed for its exploitation. (J.S.)

  4. Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis Huds.

    Science.gov (United States)

    Nađpal, Jelena D; Lesjak, Marija M; Šibul, Filip S; Anačkov, Goran T; Četojević-Simin, Dragana D; Mimica-Dukić, Neda M; Beara, Ivana N

    2016-02-01

    The aim of this study was to compare phenolic profile, vitamin C content, antioxidant, anti-inflammatory and cytotoxic activity of rose hips and the preserves (purée and jam) of two Rosa species: renowned Rosa canina L. and unexplored Rosa arvensis Huds. The liquid chromatography-tandem mass spectrometry analysis of 45 phenolics resulted in quantification of 14 compounds, with quercitrin, gallic and protocatechuic acids as the most dominant. High antioxidant potential of R. canina and a moderate activity of R. arvensis extracts were determined through several assays. Purée of both species and methanol extract of air-dried R. canina hips showed some anti-inflammatory (cyclooxygenase-1 and 12-lipooxygense inhibition potency) activity. Purée of R. canina exerted cytotoxic activity only against the HeLa cell line among several others (HeLa, MCF7, HT-29 and MRC-5). The presented results support traditional use of rose hips and their fruit preserves as food with health and nutritional benefits. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Pollination-Induced Corolla Wilting in Petunia hybrida Rapid Transfer through the Style of a Wilting-Inducing Substance.

    Science.gov (United States)

    Gilissen, L J; Hoekstra, F A

    1984-06-01

    Pollination or wounding of the stigma of Petunia hybrida flowers led to the generation of a wilting factor and its transfer to the corolla within 4 hours. This was concluded from the effects of time course removal of whole styles. In this 4-hour period, pollen tubes traversed only a fraction of the total distance to the ovaries. Both pollination and wounding of the stigma immediately resulted in an increase of ethylene evolution. Accelerated wilting, however, occured only when treated styles remained connected with the ovaries, and not when they were detached and left in the flower. A wilting factor was found in eluates collected from the ovarian end of the styles, only in the case of previous pollination or wounding. In such eluates, the level of the ethylene precursor 1-amino-cyclopropane-1-carboxylic acid was below detection.These observations suggest a material nature of the wilting factor in Petunia flowers, which rapidly passes through the style to the corolla, but which is different from 1-aminocyclopropane-1-carboxylic acid.

  6. PhEXPA1, a Petunia hybrida expansin, is involved in cell wall metabolism and in plant architecture specification.

    Science.gov (United States)

    Dal Santo, Silvia; Fasoli, Marianna; Cavallini, Erika; Tornielli, Giovanni Battista; Pezzotti, Mario; Zenoni, Sara

    2011-12-01

    Expansins are wall-loosening proteins that induce wall stress relaxation and irreversible wall extension in a pH-dependent manner. Despite a substantial body of work has been performed on the characterization of many expansins genes in different plant species, the knowledge about their precise biological roles during plant development remains scarce. To yield insights into the expansion process in Petunia hybrida, PhEXPA1, an expansin gene preferentially expressed in petal limb, has been characterized. The constitutive overexpression of PhEXPA1 significantly increased expansin activity, cells size and organ dimensions. Moreover, 35S::PhEXPA1 transgenic plants exhibited an altered cell wall polymer composition and a precocious timing of axillary meristem development compared with wild-type plants. These findings supported a previous hypothesis that expansins are not merely structural proteins involved in plant cell wall metabolism but they also take part in many plant development processes. Here, to support this expansins dual role, we discuss about differential cell wall-related genes expressed in PhEXPA1 expression mutants and gradients of altered petunia branching pattern. © 2011 Landes Bioscience

  7. Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE7 is involved in the production of negative and positive branching signals in petunia.

    Science.gov (United States)

    Drummond, Revel S M; Martínez-Sánchez, N Marcela; Janssen, Bart J; Templeton, Kerry R; Simons, Joanne L; Quinn, Brian D; Karunairetnam, Sakuntala; Snowden, Kimberley C

    2009-12-01

    One of the key factors that defines plant form is the regulation of when and where branches develop. The diversity of form observed in nature results, in part, from variation in the regulation of branching between species. Two CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes, CCD7 and CCD8, are required for the production of a branch-suppressing plant hormone. Here, we report that the decreased apical dominance3 (dad3) mutant of petunia (Petunia hybrida) results from the mutation of the PhCCD7 gene and has a less severe branching phenotype than mutation of PhCCD8 (dad1). An analysis of the expression of this gene in wild-type, mutant, and grafted petunia suggests that in petunia, CCD7 and CCD8 are coordinately regulated. In contrast to observations in Arabidopsis (Arabidopsis thaliana), ccd7ccd8 double mutants in petunia show an additive phenotype. An analysis using dad3 or dad1 mutant scions grafted to wild-type rootstocks showed that when these plants produce adventitious mutant roots, branching is increased above that seen in plants where the mutant roots are removed. The results presented here indicate that mutation of either CCD7 or CCD8 in petunia results in both the loss of an inhibitor of branching and an increase in a promoter of branching.

  8. Improving Postharvest Storage of Apple Cv.

    Directory of Open Access Journals (Sweden)

    H. Etemadi Nasab

    2013-06-01

    Full Text Available Apple is one of the important fruit in Iran. according to respiration and ethylene production behaviors, apple classified as a climacteric fruit. Therefore, any treatments, which can inhibit or prevent the action of ethylene, they can increase the postharvest life of horticultural production. 1-methylcyclopropane (1-MCP is one of the chemical which is environmental save and use as a very small concentrations. In this experiment, the effects of 1-MCP at concentrations of 0 (as a control, 0.25, 0.5, 0.75 and 1 µl.l-1 were used in apple cv. "Golab Kohanz", a land race fruits which is cultivated from long times ago in Iran. After the 1-MCP treatment, they were moved to cold room at 2 ºC and RH of ca 90% for 60 days. Samples were removed from the cold room and analysis for physicochemical characteristics. "Golab Kohanz" apples treated with 1-MCP showed improved fruit firmness, titratable acidity (TA, and total soluble solid after 147 days at 2ºC , compared to the control non-treated fruits. Also, treatment with 1-MCP significantly reduced the incidence of superficial scald in "Golab Kohanze". In general, 1-MCP-treated apples delay ripening during storing in cold room and increased shelf life. Overall, the results indicate that 1-MCP has tremendous potential for maintaining apple quality during storage and post storage.

  9. "Ishica, de quien sos tu?": Nostalgia for a Mother Tongue in Rosa Nissan's Novels

    Science.gov (United States)

    Linhard, Tabea Alex

    2009-01-01

    This paper explores the use of Judeo-Spanish in the Rosa Nissan's novels "Novia que te vea" (1992) and "Hisho que te nazca" (1996) and reveals the ways in which the presence of this language interrupts the otherwise linear coming-of-age narrative. An analysis of the main character's relationship with Judeo-Spanish establishes a critical dialogue…

  10. Heat loss and fluid leakage tests of the ROSA-III facility

    International Nuclear Information System (INIS)

    Suzuki, Mitsuhiro; Tasaka, Kanji; Shiba, Masayoshi

    1981-12-01

    The report presents characteristic test results about the steady state heat loss, one of the inherent characteristics of the ROSA-III test facility. The steady state heat loss tests were conducted at five different temperature conditions between 111 0 C and 290 0 C . Net heat loss rates were obtained by estimating the electric power supplied to the core, heat input from the recirculation pumps and steam leakage rate. The heat loss characteristics have important contribution to analyses of the ROSA-III small break tests. A following simple relation was obtained between the net heat loss rate Q*sub(HL) (kJ/s) (*: radical) of the ROSA-III facility and the temperature difference ΔT ( 0 C) between the fluid temperature of the system and the room temperature, Q*sub(HL) = 0.56 x ΔT. (*: radical) And the steam leak flow at normal operating condition of the ROSA-III test, (P = 7.2 MPa) was obtained as 8.9 x 10 -3 kg/s and corresponding steam leakage energy as 10.5 kJ/s. The heat input from the recirculation pumps was indirectly estimated under a constant speed by assuming the heat input was equal to the brake horce power of the pumps. (author)

  11. Comparison report for CSNI International Standard Problem 12 (ROSA-III Run 912)

    International Nuclear Information System (INIS)

    Tasaka, Kanji; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Iriko, Masanori; Yonomoto, Taisuke; Shiba, Masayoshi

    1982-09-01

    ROSA-III Run 912 was identified as International Standard Problem 12 by the Committee on the Safety of Nuclear Installations. Run 912 simulated a 5% split break LOCA condition in a BWR at the pump suction in the recirculation line with the HPCS failure. Comparisons between the test data and the calculations by eight international participants were made and discussed. (author)

  12. Taxonomic status of the roses (Rosa) described by S.G. Dimitrov from Bulgaria

    DEFF Research Database (Denmark)

    Zielinski, J.; Petrova, A; Tan, Kit

    2004-01-01

    The original herbarium vouchers for six species of Rosa (Rosaceae) described by S. G. Dimitrov from Bulgaria are taxonomically evaluated. Two species (R. balcanica, R. orphei) are considered hybrids, four other names (R. bulgarica, R. parilica, R. pontica and R. rhodopaea) are taxonomic synonyms...

  13. Cytotoxic Activity of the Leaf and Stem Extracts of Hibiscus rosa ...

    African Journals Online (AJOL)

    Methods: The crude petroleum ether, ethyl acetate and methanol extracts of the leaf and stem of Hibiscus rosa sinensis were prepared using cold extraction method. The in vitro cytotoxic activity of the extracts (20 - 100 μg/ml) was evaluated on leukaemic cancer cell line (K-562) and Mardin-Darby kidney cell line (MDBK) ...

  14. The Politics of Children's Literature: The Story of Rosa Parks and the Montgomery Bus Boycott.

    Science.gov (United States)

    Kohl, Herbert

    1991-01-01

    As commonly told to and read by children, the story of Rosa Parks and the Montgomery bus boycott fails to indicate Mrs. Parks' activist role or the degree of community organization and participation in the boycott. Telling what actually occurred allows children identify with people who make justice happen. (SLD)

  15. Cuticular hydrocarbons corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis rosa) populations

    Czech Academy of Sciences Publication Activity Database

    Vaníčková, Lucie; Břízová, Radka; Pompeiano, A.; Ekesi, S.; De Meyer, M.

    -, č. 540 (2015), s. 507-524 ISSN 1313-2989 Institutional support: RVO:61388963 Keywords : Ceratitis rosa * cryptic species * chemotaxonomy * GCxGC/MS * integrative taxonomy Subject RIV: EG - Zoology Impact factor: 0.938, year: 2015 http://zookeys.pensoft.net/articles.php?id=6222

  16. Photograph-based ergonomic evaluations using the Rapid Office Strain Assessment (ROSA).

    Science.gov (United States)

    Liebregts, J; Sonne, M; Potvin, J R

    2016-01-01

    The Rapid Office Strain Assessment (ROSA) was developed to assess musculoskeletal disorder (MSD) risk factors for computer workstations. This study examined the validity and reliability of remotely conducted, photo-based assessments using ROSA. Twenty-three office workstations were assessed on-site by an ergonomist, and 5 photos were obtained. Photo-based assessments were conducted by three ergonomists. The sensitivity and specificity of the photo-based assessors' ability to correctly classify workstations was 79% and 55%, respectively. The moderate specificity associated with false positive errors committed by the assessors could lead to unnecessary costs to the employer. Error between on-site and photo-based final scores was a considerable ∼2 points on the 10-point ROSA scale (RMSE = 2.3), with a moderate relationship (ρ = 0.33). Interrater reliability ranged from fairly good to excellent (ICC = 0.667-0.856) and was comparable to previous results. Sources of error include the parallax effect, poor estimations of small joint (e.g. hand/wrist) angles, and boundary errors in postural binning. While this method demonstrated potential validity, further improvements should be made with respect to photo-collection and other protocols for remotely-based ROSA assessments. Copyright © 2015 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  17. ANALISIS POSTUR KERJA PADA PT. XYZ MENGGUNAKAN METODE ROSA (RAPID OFFICE STRAIN ASSESSMENT

    Directory of Open Access Journals (Sweden)

    Rosma Hani Damayanti

    2014-06-01

    Full Text Available Penggunaan teknologi informasi, dimana komputer sebagai medianya kini semakin meningkat. Frekuensi yang tinggi akan penggunaan komputer yang tidak memperhatikan sisi ergonomi dalam bekerja mengakibatkan adanya resiko yang dirasakan oleh pengguna. PT. XYZ merupakan salah satu perusahaan yang menggunakan komputer sebagai salah satu alat utama dalam bekerja. Pada penggunaannya karyawan pada Departemen Publishing merasakan keluhan pada punggung, pinggang, nyeri bahu, leher dan tangan. Keluhan yang dirasakan oleh pekerja pada Departemen publishing dapat diminimalkan dengan cara mengetahui dan mengidentifikasi postur kerja pada pekerja dalam menggunakan komputer. Identifikasi tersebut digunakan untuk mengetahui kondisi pekerja dan mengetahui penyebab keluhan yang dirasakan oleh pekerja untuk dilakukan perbaikan. Pada penelitian ini dilakukan analisis dengan menggunakan metode ROSA untuk mengurangi adanya keluhan yang dirasakan oleh pekerja pada Departemen publishing. ROSA merupakan salah satu metode pada office ergonomics, dimana penilaiannnya dirancang untuk mengukur resiko yang terkait dengan penggunaan komputer serta untuk menetapkan tingkat tindakan perubahan berdasarkan laporan dari ketidaknyamanan pekerja. Dengan menggunakan metode ROSA, dapat diketahui apakah postur kerja karyawan pada Departemen Publishing pada saat bekerja aman atau berbahaya. Hasil analisis postur kerja menggunakan metode ROSA pada Departemen Publishing menunjukkan bahwa seluruh pekerja yang menjadi sampel memiliki level resiko yang tinggi dan perlu dilakukan perbaikan segera. Perbaikan yang dapat dilakukan untuk mengurangi tingkat resiko yang dirasakan oleh pekerja yaitu dengan perbaikan fasilitas yang digunakan oleh pekerja yang sesuai dengan standar ergonomi, melakukan sosialisasi kepada pekerja tentang pentingnya ergonomi pada dunia kerja, dan sebaiknya pekerja melakukan istirahat atau peregangan otot minimal setiap tiga jam sekali.

  18. ANALISIS POSTUR KERJA PADA PT. XYZ MENGGUNAKAN METODE ROSA (RAPID OFFICE STRAIN ASSESSMENT

    Directory of Open Access Journals (Sweden)

    Rosma Hani Damayanti

    2014-06-01

    Full Text Available Penggunaan teknologi informasi, dimana komputer sebagai medianya kini semakin meningkat. Frekuensi yang tinggi akan penggunaan komputer yang tidak memperhatikan sisi ergonomi dalam bekerja mengakibatkan adanya resiko yang dirasakan oleh pengguna. PT. XYZ merupakan salah satu perusahaan yang menggunakan komputer sebagai salah satu alat utama dalam bekerja. Pada penggunaannya karyawan pada Departemen Publishing merasakan keluhan pada punggung, pinggang, nyeri bahu, leher dan tangan. Keluhan yang dirasakan oleh pekerja pada Departemen publishing dapat diminimalkan dengan cara mengetahui dan mengidentifikasi postur kerja pada pekerja dalam menggunakan komputer. Identifikasi tersebut digunakan untuk mengetahui kondisi pekerja dan mengetahui penyebab keluhan yang dirasakan oleh pekerja untuk dilakukan perbaikan. Pada penelitian ini dilakukan analisis dengan menggunakan metode ROSA untuk mengurangi adanya keluhan yang dirasakan oleh pekerja pada Departemen publishing. ROSA merupakan salah satu metode pada office ergonomics, dimana penilaiannnya dirancang untuk mengukur resiko yang terkait dengan penggunaan komputer serta untuk menetapkan tingkat tindakan perubahan berdasarkan laporan dari ketidaknyamanan pekerja. Dengan menggunakan metode ROSA, dapat diketahui apakah postur kerja karyawan pada Departemen Publishing pada saat bekerja aman atau berbahaya. Hasil analisis postur kerja menggunakan metode ROSA pada Departemen Publishing menunjukkan bahwa seluruh pekerja yang menjadi sampel memiliki level resiko yang tinggi dan perlu dilakukan perbaikan segera.  Perbaikan yang dapat dilakukan untuk mengurangi tingkat resiko yang dirasakan oleh pekerja yaitu dengan perbaikan fasilitas yang digunakan oleh pekerja yang sesuai dengan standar ergonomi, melakukan sosialisasi kepada pekerja tentang pentingnya ergonomi pada dunia kerja, dan sebaiknya pekerja melakukan istirahat atau peregangan otot minimal setiap tiga jam sekali.

  19. INDIKATOR TITRASI ASAM-BASA DARI EKSTRAK BUNGA SEPATU (Hibiscus rosa sinensis L Indicator of Acid-Base Titration from the Extract of Hibiscus rosa sinensis L Flower

    Directory of Open Access Journals (Sweden)

    Siti Nuryanti

    2012-05-01

    Full Text Available Titration acid-base needs indicator  to show the change of color on interval of hydrogen exponent/degree of acid (pH. Indicator of synthetic which always be used have disadvantages like chemical pollution,  stock and expensive of pro- duction  cost. The research has been carried out to substitute the synthetic indicator with herbal indicator extracted from flower crown of Hibiscus rosa sinensis L extract. The herbal indicator was extracted from the flower crown Hibiscus rosa sinensis L using a mixture methanol-acetic acid. Then it was evaluated with phenolphthalein and methyl orange(E merck comparer to titration the acid-base, they are strong acid-strong base, weak base-strong acid and weak acid-strong base. The result of research show that herbal indicator  of flower crown Hibiscus rosa sinensis L to show theequivalent point in all titrations give peer result with the comparison. With the research result hoped that indicatortitration acid-base flower crown Hibiscus rosa sinensis L is able to as replace synthetic indicator  (metyl orange andphenolphtalein which always be used before. ABSTRAK Titrasi asam-basa memerlukan indikator untuk menunjukkan perubahan warna pada setiap interval derajad keasaman (pH. Indikator sintetis yang digunakan selama ini mempunyai beberapa kelemahan seperti polusi kimia, ketersediaan dan biaya produksi mahal. Upaya penelitian sudah dilakukan untuk menggantikan indikator sintetis dengan indikator dari ekstrak mahkota bunga sepatu. Indikator herbal tersebut dibuat dengan cara mengekstrak mahkota bunga Hibiscus rosa sinensis L dengan mengunakan pelarut metanol-asam asetat. Kemudian dievaluasi dengan indikator pembanding fenolftalein dan metil oranye (produksi E merck untuk titrasi asam-basa yaitu asam kuat-basa kuat, basa lemah-asam kuat dan asam lemah-basa kuat. Dari hasil penelitian diketahui bahwa indikator dari mahkota bunga sepatu untuk menunjukkan titik ekivalen dalam titrasi tersebut memberikan hasil yang

  20. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Science.gov (United States)

    Afiune, Luana Alves Freitas; Leal-Silva, Thaís; Sinzato, Yuri Karen; Moraes-Souza, Rafaianne Queiroz; Soares, Thaigra Sousa; Campos, Kleber Eduardo; Fujiwara, Ricardo Toshio; Herrera, Emilio; Damasceno, Débora Cristina; Volpato, Gustavo Tadeu

    2017-01-01

    The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes. Diabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed. The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group. Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  1. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes

    Science.gov (United States)

    Afiune, Luana Alves Freitas; Leal-Silva, Thaís; Sinzato, Yuri Karen; Moraes-Souza, Rafaianne Queiroz; Soares, Thaigra Sousa; Campos, Kleber Eduardo; Fujiwara, Ricardo Toshio; Herrera, Emilio; Damasceno, Débora Cristina

    2017-01-01

    Purpose The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes. Methods Diabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed. Results The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group. Conclusion Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy. PMID:28644857

  2. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Directory of Open Access Journals (Sweden)

    Luana Alves Freitas Afiune

    Full Text Available The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes.Diabetes was induced by streptozotocin (STZ, 40 mg/kg in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group: non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed.The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI and coronary artery risk index (CRI, and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group.Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  3. A practical introduction to computer vision with OpenCV

    CERN Document Server

    Dawson-Howe, Kenneth

    2014-01-01

    Explains the theory behind basic computer vision and provides a bridge from the theory to practical implementation using the industry standard OpenCV libraries Computer Vision is a rapidly expanding area and it is becoming progressively easier for developers to make use of this field due to the ready availability of high quality libraries (such as OpenCV 2).  This text is intended to facilitate the practical use of computer vision with the goal being to bridge the gap between the theory and the practical implementation of computer vision. The book will explain how to use the relevant OpenCV

  4. Guidelines on CV networking information flow optimization for Texas.

    Science.gov (United States)

    2017-03-01

    Recognizing the fundamental role of information flow in future transportation applications, the research team investigated the quality and security of information flow in the connected vehicle (CV) environment. The research team identified key challe...

  5. Calibration of ADRET voltage generator type CV102. Program CODAV

    International Nuclear Information System (INIS)

    Lagarde, Gerard.

    1978-07-01

    The CODAV programm studied by the Metrology SES/SME laboratory is used for the calibration of ADRET voltage generator type CV.102. A JCAM.10 microcomputer run the measurement cycle and the printout of the results [fr

  6. Nutritive evaluation of Medicago truncutula (cv. jernalong) pasture ...

    African Journals Online (AJOL)

    Nutritive evaluation of Medicago truncutula (cv. jernalong) pasture for sheep. 1. Seasonal .... obtained by laboratory work, using in vitro techniques. (Engels et al. .... model that was used to explain 92,3% of the variance in. IVDOM content.

  7. Seafloor Backscatter Image of North of Santa Rosa Island, Channel Islands National Marine Sanctuary (8m resolution tif)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This image represents an 8 meter resolution backscatter of the seafloor south of Santa Rosa Island in Channel Islands National Marine Sanctuary. It was acquired...

  8. Seafloor Backscatter Image of South of Santa Rosa Island, Channel Islands National Marine Sanctuary (8m resolution tif)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This image represents an 8 meter resolution backscatter of the seafloor south of Santa Rosa Island in Channel Islands National Marine Sanctuary. It was acquired...

  9. Recirculation pump suction line 2.8% break integral test at ROSA-III with HPCS failure, RUN 984

    International Nuclear Information System (INIS)

    Suzuki, Mitsuhiro; Anoda, Yoshinari; Tasaka, Kanji; Kumamaru, Hiroshige; Nakamura, Hideo; Yonomoto, Taisuke; Murata, Hideo; Shiba, Masayoshi

    1984-06-01

    This report presents the experimental data of 2.8% suction line break test RUN 984 at ROSA-III, which was conducted as one of counterpart tests to FIST program sponsored by GE, EPRI and USNRC. The similarity study between the ROSA-III and FIST tests is on the way. The report also presents the information on the ROSA-III test facility, experiment results and the effects of the ADS flow rate and the MSIV trip level comparing with the previously conducted ROSA-III small break tests, RUNs 920 and 922. Major conclusions obtained are as follows. (1) Change of the MSIV trip level from L2 to L1 gives delay of MSIV closure and longer actuation of pressure control system in a small break LOCA. (2) Larger ADS flow gives faster depressurization rate and earlier ECCS actuation, which results in shorter fuel rod dryout period and lower PCT. (author)

  10. Seafloor Bathymetry Image of South of Santa Rosa Island, Channel Islands National Marine Sanctuary (8m resolution tif)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This image represents an 8 meter resolution bathymetry of the seafloor south of Santa Rosa Island in Channel Islands National Marine Sanctuary. It was acquired using...

  11. Seafloor Bathymetry Image of North of Santa Rosa Island, Channel Islands National Marine Sanctuary (8m resolution tif)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This image represents an 8 meter resolution bathymetry of the seafloor north of Santa Rosa Island in Channel Islands National Marine Sanctuary. It was acquired using...

  12. Dissecting Tissue-Specific Transcriptomic Responses from Leaf and Roots under Salt Stress in Petunia hybrida Mitchell

    Science.gov (United States)

    Villarino, Gonzalo H.; Hu, Qiwen; Scanlon, Michael J.; Mueller, Lukas; Mattson, Neil S.

    2017-01-01

    One of the primary objectives of plant biotechnology is to increase resistance to abiotic stresses, such as salinity. Salinity is a major abiotic stress and increasing crop resistant to salt continues to the present day as a major challenge. Salt stress disturbs cellular environment leading to protein misfolding, affecting normal plant growth and causing agricultural losses worldwide. The advent of state-of-the-art technologies such as high throughput mRNA sequencing (RNA-seq) has revolutionized whole-transcriptome analysis by allowing, with high precision, to measure changes in gene expression. In this work, we used tissue-specific RNA-seq to gain insight into the Petunia hybrida transcriptional responses under NaCl stress using a controlled hydroponic system. Roots and leaves samples were taken from a continuum of 48 h of acute 150 mM NaCl. This analysis revealed a set of tissue and time point specific differentially expressed genes, such as genes related to transport, signal transduction, ion homeostasis as well as novel and undescribed genes, such as Peaxi162Scf00003g04130 and Peaxi162Scf00589g00323 expressed only in roots under salt stress. In this work, we identified early and late expressed genes in response to salt stress while providing a core of differentially express genes across all time points and tissues, including the trehalose-6-phosphate synthase 1 (TPS1), a glycosyltransferase reported in salt tolerance in other species. To test the function of the novel petunia TPS1 allele, we cloned and showed that TPS1 is a functional plant gene capable of complementing the trehalose biosynthesis pathway in a yeast tps1 mutant. The list of candidate genes to enhance salt tolerance provided in this work constitutes a major effort to better understand the detrimental effects of salinity in petunia with direct implications for other economically important Solanaceous species. PMID:28771200

  13. Comprehensive Transcriptome Analysis Unravels the Existence of Crucial Genes Regulating Primary Metabolism during Adventitious Root Formation in Petunia hybrida

    Science.gov (United States)

    Ahkami, Amirhossein; Scholz, Uwe; Steuernagel, Burkhard; Strickert, Marc; Haensch, Klaus-Thomas; Druege, Uwe; Reinhardt, Didier; Nouri, Eva; von Wirén, Nicolaus; Franken, Philipp; Hajirezaei, Mohammad-Reza

    2014-01-01

    To identify specific genes determining the initiation and formation of adventitious roots (AR), a microarray-based transcriptome analysis in the stem base of the cuttings of Petunia hybrida (line W115) was conducted. A microarray carrying 24,816 unique, non-redundant annotated sequences was hybridized to probes derived from different stages of AR formation. After exclusion of wound-responsive and root-regulated genes, 1,354 of them were identified which were significantly and specifically induced during various phases of AR formation. Based on a recent physiological model distinguishing three metabolic phases in AR formation, the present paper focuses on the response of genes related to particular metabolic pathways. Key genes involved in primary carbohydrate metabolism such as those mediating apoplastic sucrose unloading were induced at the early sink establishment phase of AR formation. Transcriptome changes also pointed to a possible role of trehalose metabolism and SnRK1 (sucrose non-fermenting 1- related protein kinase) in sugar sensing during this early step of AR formation. Symplastic sucrose unloading and nucleotide biosynthesis were the major processes induced during the later recovery and maintenance phases. Moreover, transcripts involved in peroxisomal beta-oxidation were up-regulated during different phases of AR formation. In addition to metabolic pathways, the analysis revealed the activation of cell division at the two later phases and in particular the induction of G1-specific genes in the maintenance phase. Furthermore, results point towards a specific demand for certain mineral nutrients starting in the recovery phase. PMID:24978694

  14. Functional diversification of duplicated CYC2 clade genes in regulation of inflorescence development in Gerbera hybrida (Asteraceae).

    Science.gov (United States)

    Juntheikki-Palovaara, Inka; Tähtiharju, Sari; Lan, Tianying; Broholm, Suvi K; Rijpkema, Anneke S; Ruonala, Raili; Kale, Liga; Albert, Victor A; Teeri, Teemu H; Elomaa, Paula

    2014-09-01

    The complex inflorescences (capitula) of Asteraceae consist of different types of flowers. In Gerbera hybrida (gerbera), the peripheral ray flowers are bilaterally symmetrical and lack functional stamens while the central disc flowers are more radially symmetrical and hermaphroditic. Proteins of the CYC2 subclade of the CYC/TB1-like TCP domain transcription factors have been recruited several times independently for parallel evolution of bilaterally symmetrical flowers in various angiosperm plant lineages, and have also been shown to regulate flower-type identity in Asteraceae. The CYC2 subclade genes in gerbera show largely overlapping gene expression patterns. At the level of single flowers, their expression domain in petals shows a spatial shift from the dorsal pattern known so far in species with bilaterally symmetrical flowers, suggesting that this change in expression may have evolved after the origin of Asteraceae. Functional analysis indicates that GhCYC2, GhCYC3 and GhCYC4 mediate positional information at the proximal-distal axis of the inflorescence, leading to differentiation of ray flowers, but that they also regulate ray flower petal growth by affecting cell proliferation until the final size and shape of the petals is reached. Moreover, our data show functional diversification for the GhCYC5 gene. Ectopic activation of GhCYC5 increases flower density in the inflorescence, suggesting that GhCYC5 may promote the flower initiation rate during expansion of the capitulum. Our data thus indicate that modification of the ancestral network of TCP factors has, through gene duplications, led to the establishment of new expression domains and to functional diversification. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.

  15. Transcriptome, carbohydrate, and phytohormone analysis of Petunia hybrida reveals a complex disturbance of plant functional integrity under mild chilling stress

    Science.gov (United States)

    Bauerfeind, Martin Andreas; Winkelmann, Traud; Franken, Philipp; Druege, Uwe

    2015-01-01

    Cultivation of chilling-tolerant ornamental crops at lower temperature could reduce the energy demands of heated greenhouses. To provide a better understanding of how sub-optimal temperatures (12°C vs. 16°C) affect growth of the sensitive Petunia hybrida cultivar ‘SweetSunshine Williams’, the transcriptome, carbohydrate metabolism, and phytohormone homeostasis were monitored in aerial plant parts over 4 weeks by use of a microarray, enzymatic assays and GC-MS/MS. The data revealed three consecutive phases of chilling response. The first days were marked by a strong accumulation of sugars, particularly in source leaves, preferential up-regulation of genes in the same tissue and down-regulation of several genes in the shoot apex, especially those involved in the abiotic stress response. The midterm phase featured a partial normalization of carbohydrate levels and gene expression. After 3 weeks of chilling exposure, a new stabilized balance was established. Reduced hexose levels in the shoot apex, reduced ratios of sugar levels between the apex and source leaves and a higher apical sucrose/hexose ratio, associated with decreased activity and expression of cell wall invertase, indicate that prolonged chilling induced sugar accumulation in source leaves at the expense of reduced sugar transport to and reduced sucrose utilization in the shoot. This was associated with reduced levels of indole-3-acetic acid and abscisic acid in the apex and high numbers of differentially, particularly up-regulated genes, especially in the source leaves, including those regulating histones, ethylene action, transcription factors, and a jasmonate-ZIM-domain protein. Transcripts of one Jumonji C domain containing protein and one expansin accumulated in source leaves throughout the chilling period. The results reveal a dynamic and complex disturbance of plant function in response to mild chilling, opening new perspectives for the comparative analysis of differently tolerant cultivars

  16. Role of the GRAS transcription factor ATA/RAM1 in the transcriptional reprogramming of arbuscular mycorrhiza in Petunia hybrida.

    Science.gov (United States)

    Rich, Mélanie K; Courty, Pierre-Emmanuel; Roux, Christophe; Reinhardt, Didier

    2017-08-08

    Development of arbuscular mycorrhiza (AM) requires a fundamental reprogramming of root cells for symbiosis. This involves the induction of hundreds of genes in the host. A recently identified GRAS-type transcription factor in Petunia hybrida, ATA/RAM1, is required for the induction of host genes during AM, and for morphogenesis of the fungal endosymbiont. To better understand the role of RAM1 in symbiosis, we set out to identify all genes that depend on activation by RAM1 in mycorrhizal roots. We have carried out a transcript profiling experiment by RNAseq of mycorrhizal plants vs. non-mycorrhizal controls in wild type and ram1 mutants. The results show that the expression of early genes required for AM, such as the strigolactone biosynthetic genes and the common symbiosis signalling genes, is independent of RAM1. In contrast, genes that are involved at later stages of symbiosis, for example for nutrient exchange in cortex cells, require RAM1 for induction. RAM1 itself is highly induced in mycorrhizal roots together with many other transcription factors, in particular GRAS proteins. Since RAM1 has previously been shown to be directly activated by the common symbiosis signalling pathway through CYCLOPS, we conclude that it acts as an early transcriptional switch that induces many AM-related genes, among them genes that are essential for the development of arbuscules, such as STR, STR2, RAM2, and PT4, besides hundreds of additional RAM1-dependent genes the role of which in symbiosis remains to be explored. Taken together, these results indicate that the defect in the morphogenesis of the fungal arbuscules in ram1 mutants may be an indirect consequence of functional defects in the host, which interfere with nutrient exchange and possibly other functions on which the fungus depends.

  17. Comprehensive transcriptome analysis unravels the existence of crucial genes regulating primary metabolism during adventitious root formation in Petunia hybrida.

    Science.gov (United States)

    Ahkami, Amirhossein; Scholz, Uwe; Steuernagel, Burkhard; Strickert, Marc; Haensch, Klaus-Thomas; Druege, Uwe; Reinhardt, Didier; Nouri, Eva; von Wirén, Nicolaus; Franken, Philipp; Hajirezaei, Mohammad-Reza

    2014-01-01

    To identify specific genes determining the initiation and formation of adventitious roots (AR), a microarray-based transcriptome analysis in the stem base of the cuttings of Petunia hybrida (line W115) was conducted. A microarray carrying 24,816 unique, non-redundant annotated sequences was hybridized to probes derived from different stages of AR formation. After exclusion of wound-responsive and root-regulated genes, 1,354 of them were identified which were significantly and specifically induced during various phases of AR formation. Based on a recent physiological model distinguishing three metabolic phases in AR formation, the present paper focuses on the response of genes related to particular metabolic pathways. Key genes involved in primary carbohydrate metabolism such as those mediating apoplastic sucrose unloading were induced at the early sink establishment phase of AR formation. Transcriptome changes also pointed to a possible role of trehalose metabolism and SnRK1 (sucrose non-fermenting 1- related protein kinase) in sugar sensing during this early step of AR formation. Symplastic sucrose unloading and nucleotide biosynthesis were the major processes induced during the later recovery and maintenance phases. Moreover, transcripts involved in peroxisomal beta-oxidation were up-regulated during different phases of AR formation. In addition to metabolic pathways, the analysis revealed the activation of cell division at the two later phases and in particular the induction of G1-specific genes in the maintenance phase. Furthermore, results point towards a specific demand for certain mineral nutrients starting in the recovery phase.

  18. Comprehensive transcriptome analysis unravels the existence of crucial genes regulating primary metabolism during adventitious root formation in Petunia hybrida.

    Directory of Open Access Journals (Sweden)

    Amirhossein Ahkami

    Full Text Available To identify specific genes determining the initiation and formation of adventitious roots (AR, a microarray-based transcriptome analysis in the stem base of the cuttings of Petunia hybrida (line W115 was conducted. A microarray carrying 24,816 unique, non-redundant annotated sequences was hybridized to probes derived from different stages of AR formation. After exclusion of wound-responsive and root-regulated genes, 1,354 of them were identified which were significantly and specifically induced during various phases of AR formation. Based on a recent physiological model distinguishing three metabolic phases in AR formation, the present paper focuses on the response of genes related to particular metabolic pathways. Key genes involved in primary carbohydrate metabolism such as those mediating apoplastic sucrose unloading were induced at the early sink establishment phase of AR formation. Transcriptome changes also pointed to a possible role of trehalose metabolism and SnRK1 (sucrose non-fermenting 1- related protein kinase in sugar sensing during this early step of AR formation. Symplastic sucrose unloading and nucleotide biosynthesis were the major processes induced during the later recovery and maintenance phases. Moreover, transcripts involved in peroxisomal beta-oxidation were up-regulated during different phases of AR formation. In addition to metabolic pathways, the analysis revealed the activation of cell division at the two later phases and in particular the induction of G1-specific genes in the maintenance phase. Furthermore, results point towards a specific demand for certain mineral nutrients starting in the recovery phase.

  19. Dissecting Tissue-Specific Transcriptomic Responses from Leaf and Roots under Salt Stress in Petunia hybrida Mitchell

    Directory of Open Access Journals (Sweden)

    Gonzalo H. Villarino

    2017-08-01

    Full Text Available One of the primary objectives of plant biotechnology is to increase resistance to abiotic stresses, such as salinity. Salinity is a major abiotic stress and increasing crop resistant to salt continues to the present day as a major challenge. Salt stress disturbs cellular environment leading to protein misfolding, affecting normal plant growth and causing agricultural losses worldwide. The advent of state-of-the-art technologies such as high throughput mRNA sequencing (RNA-seq has revolutionized whole-transcriptome analysis by allowing, with high precision, to measure changes in gene expression. In this work, we used tissue-specific RNA-seq to gain insight into the Petunia hybrida transcriptional responses under NaCl stress using a controlled hydroponic system. Roots and leaves samples were taken from a continuum of 48 h of acute 150 mM NaCl. This analysis revealed a set of tissue and time point specific differentially expressed genes, such as genes related to transport, signal transduction, ion homeostasis as well as novel and undescribed genes, such as Peaxi162Scf00003g04130 and Peaxi162Scf00589g00323 expressed only in roots under salt stress. In this work, we identified early and late expressed genes in response to salt stress while providing a core of differentially express genes across all time points and tissues, including the trehalose-6-phosphate synthase 1 (TPS1, a glycosyltransferase reported in salt tolerance in other species. To test the function of the novel petunia TPS1 allele, we cloned and showed that TPS1 is a functional plant gene capable of complementing the trehalose biosynthesis pathway in a yeast tps1 mutant. The list of candidate genes to enhance salt tolerance provided in this work constitutes a major effort to better understand the detrimental effects of salinity in petunia with direct implications for other economically important Solanaceous species.

  20. Small RNA and transcriptome deep sequencing proffers insight into floral gene regulation in Rosa cultivars

    Directory of Open Access Journals (Sweden)

    Kim Jungeun

    2012-11-01

    Full Text Available Abstract Background Roses (Rosa sp., which belong to the family Rosaceae, are the most economically important ornamental plants—making up 30% of the floriculture market. However, given high demand for roses, rose breeding programs are limited in molecular resources which can greatly enhance and speed breeding efforts. A better understanding of important genes that contribute to important floral development and desired phenotypes will lead to improved rose cultivars. For this study, we analyzed rose miRNAs and the rose flower transcriptome in order to generate a database to expound upon current knowledge regarding regulation of important floral characteristics. A rose genetic database will enable comprehensive analysis of gene expression and regulation via miRNA among different Rosa cultivars. Results We produced more than 0.5 million reads from expressed sequences, totalling more than 110 million bp. From these, we generated 35,657, 31,434, 34,725, and 39,722 flower unigenes from Rosa hybrid: ‘Vital’, ‘Maroussia’, and ‘Sympathy’ and Rosa rugosa Thunb. , respectively. The unigenes were assigned functional annotations, domains, metabolic pathways, Gene Ontology (GO terms, Plant Ontology (PO terms, and MIPS Functional Catalogue (FunCat terms. Rose flower transcripts were compared with genes from whole genome sequences of Rosaceae members (apple, strawberry, and peach and grape. We also produced approximately 40 million small RNA reads from flower tissue for Rosa, representing 267 unique miRNA tags. Among identified miRNAs, 25 of them were novel and 242 of them were conserved miRNAs. Statistical analyses of miRNA profiles revealed both shared and species-specific miRNAs, which presumably effect flower development and phenotypes. Conclusions In this study, we constructed a Rose miRNA and transcriptome database, and we analyzed the miRNAs and transcriptome generated from the flower tissues of four Rosa cultivars. The database provides a

  1. Effects of Herbal-Acupuncture with Rosae Laevigatae Fructus Extract at KI10(Umgok on Osteoporosis in Ovariectomized Mice

    Directory of Open Access Journals (Sweden)

    Kim Dong-soo

    2009-12-01

    Full Text Available Objective & Methods : The purpose of this study was to observe the effects of herbal-acupuncture with Rosae Laevigatae Fructus extact(RLF-HA at KI10(Umgok on osteoporosis in ovariectomized(OVX ddy mice. We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : 1. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok and saline injection at KI10(Umgok significantly inhibited the reduction of tibial calcium level in ovariectomized mice. 2. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok significantly inhibited the increase of tibial osteoclast cells in ovariectomized mice. 3. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok significantly inhibited the reduction of tibial trabecular bone thickness(TBT in ovariectomized mice. 4. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok significantly inhibited the overgrowth of tibial growth plate length(GPL in ovariectomized mice. Conclusion : These results suggested that herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok has a therapeutic effect on osteoporosis, and to be put to practical use in the future osteoporosis clinic.

  2. Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato.

    Science.gov (United States)

    Kobayashi, Toshihiro; Kato-Itoh, Megumi; Yamaguchi, Tomoyuki; Tamura, Chihiro; Sanbo, Makoto; Hirabayashi, Masumi; Nakauchi, Hiromitsu

    2012-11-01

    Recent discovery of a method for derivation and culture of germline-competent rat pluripotent stem cells (PSCs) enables generation of transgenic rats or knock-out rats via genetic modification of such PSCs. This opens the way to use rats, as is routine in mice, for analyses of gene functions or physiological features. In mouse or human, one widely used technique to express a gene of interest stably and ubiquitously is to insert that gene into the Rosa26 locus via gene targeting of PSCs. Rosa26 knock-in mice conditionally expressing a reporter or a toxin gene have contributed to tracing or ablation of specific cell lineages. We successfully identified a rat orthologue of the mouse Rosa26 locus. Insertion of tdTomato, a variant of red fluorescent protein, into the Rosa26 locus of PSCs of various rat strains allows ubiquitous expression of tdTomato. Through germline transmission of one Rosa26-tdTomato knock-in embryonic stem cell line, we also obtained tdTomato knock-in rats. These expressed tdTomato ubiquitously throughout their bodies, which indicates that the rat Rosa26 locus conserves functions of its orthologues in mouse and human. The new tools described here (targeting vectors, knock-in PSCs, and rats) should be useful for a variety of research using rats.

  3. Nietzsche, Walter Benjamin, Guimarães Rosa: uma ideia de história = Nietzsche, Walter Benjamin, Guimarães Rosa: an idea of History

    Directory of Open Access Journals (Sweden)

    Roncari, Luiz

    2012-01-01

    Full Text Available Estudo da obra Corpo de baile, de Guimarães Rosa buscando perceber como a dispersão das personagens da primeira estória, seus reagrupamentos nas novelas intermediárias e a reintegração final, formam as ranhuras que amarravam o conjunto. Além de analisar como esses elementos de composição, que sustentam a unidade do livro, têm também um fundo ideológico, ou seja, apresentam uma visão da história e uma concepção da vida e do mundo

  4. A Mini Zinc-Finger Protein (MIF from Gerbera hybrida Activates the GASA Protein Family Gene, GEG, to Inhibit Ray Petal Elongation

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    Meixiang Han

    2017-09-01

    Full Text Available Petal appearance is an important horticultural trail that is generally used to evaluate the ornamental value of plants. However, knowledge of the molecular regulation of petal growth is mostly derived from analyses of Arabidopsis thaliana, and relatively little is known about this process in ornamental plants. Previously, GEG (Gerbera hybrida homolog of the gibberellin [GA]–stimulated transcript 1 [GAST1] from tomato, a gene from the GA stimulated Arabidopsis (GASA family, was reported to be an inhibitor of ray petal growth in the ornamental species, G. hybrida. To explore the molecular regulatory mechanism of GEG in petal growth inhibition, a mini zinc-finger protein (MIF was identified using yeast one-hybrid (Y1H screen. The direct binding of GhMIF to the GEG promoter was verified by using an electrophoretic mobility shift assay and a dual-luciferase assay. A yeast two-hybrid (Y2H revealed that GhMIF acts as a transcriptional activator. Transient transformation assay indicated that GhMIF is involved in inhibiting ray petal elongation by activating the expression of GEG. Spatiotemporal expression analyses and hormone treatment assay showed that the expression of GhMIF and GEG is coordinated during petal development. Taken together, these results suggest that GhMIF acts as a direct transcriptional activator of GEG, a gene from the GASA protein family to regulate the petal elongation.

  5. The optimization of essential oils supercritical CO2 extraction from Lavandula hybrida through static-dynamic steps procedure and semi-continuous technique using response surface method

    Science.gov (United States)

    Kamali, Hossein; Aminimoghadamfarouj, Noushin; Golmakani, Ebrahim; Nematollahi, Alireza

    2015-01-01

    Aim: The aim of this study was to examine and evaluate crucial variables in essential oils extraction process from Lavandula hybrida through static-dynamic and semi-continuous techniques using response surface method. Materials and Methods: Essential oil components were extracted from Lavandula hybrida (Lavandin) flowers using supercritical carbon dioxide via static-dynamic steps (SDS) procedure, and semi-continuous (SC) technique. Results: Using response surface method the optimum extraction yield (4.768%) was obtained via SDS at 108.7 bar, 48.5°C, 120 min (static: 8×15), 24 min (dynamic: 8×3 min) in contrast to the 4.620% extraction yield for the SC at 111.6 bar, 49.2°C, 14 min (static), 121.1 min (dynamic). Conclusion: The results indicated that a substantial reduction (81.56%) solvent usage (kg CO2/g oil) is observed in the SDS method versus the conventional SC method. PMID:25598636

  6. The chalcone synthase multigene family of Petunia hybrida (V30): differential, light-regulated expression during flower development and UV light induction

    International Nuclear Information System (INIS)

    Koes, R.E.; Spelt, C.E.; Mol, J.N.M.

    1989-01-01

    We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. About 90% of the total CHS mRNA pool is transcribed from CHS-A, wheares CHS-J delivers about 10% in flower corolla, tube and anthers. Expression of CHS-A and CHS-J during flower development is coordinated and (red) light-dependent. In young seedlings and cell suspension cultures expression of CHS-A and CHS-J can be induced with UV light. In addition to CHS-A and CHS-J, expression of another two CHS genes (CHS-B and CHS-G) is induced in young seedlings by UV light, albeit at a low level. In contrast to CHS genes from Leguminoseae, Petunia CHS genes are not inducible by phytopathogen-derived elicitors. Expression of CHS-A and CHS-J is reduced to a similar extent in a regulatory CHS mutant, Petunia hybrida Red Star, suggesting that both genes are regulated by the same trans-acting factors. Comparison of the promoter sequences of CHS-A and CHS-J reveals some striking homologies, which might represent cis-acting regulatory sequences. (author)

  7. Western Imperialism Against Primitive Communism. A New Reading of Rosa Luxemburg’s Economic Writings

    Directory of Open Access Journals (Sweden)

    Michael Löwy

    2012-01-01

    Full Text Available The discussion on Rosa Luxemburg’s theories of imperialism hasmainly focused on the economic argument - the schemes of reproduction, theprocess of circulation, the need for “external” markets, etc. There is howeveranother dimension, at least as important : the struggle of imperialism againstpre-capitalist economies, the ruthless destruction of “natural” and peasant economies,many of them being forms of primitive communism. Luxemburg’sinterest for primitive communist societies is documented by her Introductionto Political Economy and the imperialist war against them is discussed both inthis work and in the last chapters of The Accumulation of Capital. A whollyoriginal approach to the evolution of social formations, running counter tolinear “progressive” views of bourgeois ideology, is outlined in these reflections. Present indigenous struggles e.g. in Latin America, against multinational oil or mining companies, illustrate the topicality of Rosa Luxemburg’s argument in the 21th century.

  8. ROSA-III 200% double-ended break integral test RUN 901

    International Nuclear Information System (INIS)

    Nakamura, Hideo; Tasaka, Kanji; Koizumi, Yasuo; Anoda, Yoshinari; Kumamaru, Hiroshige; Murata, Hideo; Suzuki, Mitsuhiro; Shiba, Masayoshi

    1984-02-01

    This report presents the experimental data of RUN 901, a 200% double-ended break test at the recirculation pump suction line with the ROSA-III test facility. The ROSA-III test facility is a volumetrically scaled (1/424) system of the BWR/6. The facility has the electrically heated core, the break simulator and the scaled ECCS (Emergency Core Cooling System). The MSIV closure and the ECCS actuation were tripped by the liquid level in the upper downcomer. The channel inlet flows were measured by differential pressure transducers installed at the channel inlet orifices of the fuel assembly No.4. The PCT (Peak Cladding Temperature) was 780 K occured during the blowdown phase in RUN 901. The whole core was quenched after the ECCS actuation and the effectiveness of ECCS has been confirmed. (author)

  9. Data report on spray cooling test by ROSA-III, (1)

    International Nuclear Information System (INIS)

    Sobajima, Makoto; Murata, Hideo; Shiba, Masayoshi

    1980-03-01

    A separate effect test on spray cooling was carried out using one core channel of ROSA-III BWR LOCA test facility. This report describes a heating experiment in the series of runs. (1) The cooling from top of the core by spray easily causes countercurrent flow limit due to the vaparization of falling water itself, so it becomes in effective. (2) The cooling by falling water is irregular and unstable. Therefore, the cooling by the falling water is not to be relied on. (3) CCFL at porous plate is hard to occur, compared with single pipe. A quantitative study of this is desired to evaluate reflooding rate. Some suggestions for ROSA-III design are also made. (author)

  10. THE ROLE OF EMPLOYEE PERFORMANCE APPRAISAL AT CASA DE LA ROSA TIMISOARA

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    SILVIA VLAD

    2015-04-01

    Full Text Available The purpose of this research is to analyze the most important aspects of employee performance appraisal role in a hotel. The research is based on highlighting the major aspects of employee performance appraisal at Casa de la Rosa Hotel and aims the aspects related to the hotel performance. The aim of this evaluation is to observe and to analyze the employee time evolution and the way they complete their tasks and their job requirements.

  11. Hibiscus Rosa Sinensis Leaves: Analysis Of Proximate, Antioxidant Activities And Inorganic Compound

    International Nuclear Information System (INIS)

    Saiful Irwan Zubairi; Nurul Shahreda Jaies

    2014-01-01

    A variety of herbal plants species has been used in traditional medicine. Most of these plants contained several potent bio-active ingredients and nutrients that could give potential positive effects to the health such as antioxidant and antipyretic. Hibiscus rosa sinensis, commonly known as Bunga raya, have similar concoction characteristic to tea which contain antioxidants that help to control cholesterol. In addition, mucilage that was found in the leaves could helps to reduce extreme body heat during fever (which potentially acts as an antipyretic). Therefore, this preliminary study on the fresh and dried H. rosa sinensis leaves was carried out to analyze and identify the nutrients content, anti-oxidants and inorganic material. Total phenolic content (TPC) method was used for both fresh and dried leaves prior to the antioxidant activities of DPPH free radicals scavenging and ferric ion reducing antioxidant power (FRAP) as to confirm the existence of antioxidant constituents. Meanwhile, the composition of heavy metals was studied using inductively coupled plasma mass spectrometry (ICP-MS). The proximate analysis of the fresh leaves showed the presence of moisture content (9.03 %), protein (10.44 %), fat (6.43 %), crude fiber (11.55 %), ash (11.22 %) and carbohydrate (51.33 %). Meanwhile, the inorganic contents are as follows: cadmium (Cd), chromium (Cr), arsenic (As), nickel (Ni), lead (Pb), iron (Fe) and zinc (Zn). Furthermore, the antioxidant activities of FRAP and DPPH showed that dried leaves of H. rosa sinensis was higher than the fresh leaves (p < 0.05) irrespective of any solvent used. The positive correlation between TPC and two other antioxidant activities of DPPH and FRAP (p < 0.05) indicates the presence of antioxidant components in the acetone and water extracts. Therefore, the high availability of essential nutritional component, anti-oxidants and low concentration of hazardous inorganic matter in H. rosa sinensis leaves enable it to be used as one

  12. Antihyperlipidemic activity of Hibiscus rosa-sinensis Linn. ethanolic extract fractions

    OpenAIRE

    Mukesh Singh Sikarwar; M B Patil

    2015-01-01

    Aim: The study investigates the antihyperlipidemic effect of Hibiscus rosa-sinensis Linn. (Malvaceae) ethanolic extract fractions in triton and atherogenic diet-induced hyperlipidemic rats. Materials and Methods: Oral administrations of 500 mg/kg body weight of various fractions of selected plant were evaluated for possible antihyperlipidemic activity in triton and atherogenic diet-induced hyperlipidemic rats for duration of 48 h and 14 days respectively. In triton model, hyperlipidemia was i...

  13. Sobre a obscenidade inocente: o caderno Rosa de Lori Lamby, de Hilda Hilst

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    Luciana Borges

    2006-01-01

    Full Text Available From this text gender construction and pornography on female literature may be discussed. By reading O caderno rosa de Lori Lamby, not only the ancient prejudice over the erotic literature (what transforms written pornography into outraging political act but the way society assemble the idea that eroticism and sexual army may raise once it’s associated with the contrasts between male and female conceptions.

  14. Experiment on performance of upper head injection system with ROSA-II

    International Nuclear Information System (INIS)

    1978-05-01

    Of the total 10 ROSA-II/UHI performance tests, 6 were reported previously. The rest are presented and discussion is made on the effects of heat generation in the core and UHI injection and repeatability of experiments. In addition, the following are described: (1) Pressure spikes observed in the upper head after sudden stoppage of UHI injection, and (2) discharge flow oscillation possibly due to UHI water injection into the upper plenum. (auth.)

  15. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

    OpenAIRE

    Kaleemullah, M.; Jiyauddin, K.; Thiban, E.; Rasha, S.; Al-Dhalli, S.; Budiasih, S.; Gamal, O.E.; Fadli, A.; Eddy, Y.

    2016-01-01

    Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop susta...

  16. The effect of aromatherapy with Rosa damascena essential oil on sleep quality in children

    OpenAIRE

    A.S. Keyhanmehr; M. Movahhed; S. Sahranavard; L. Gachkar; M. Hamdieh; Sh. Afsharpaiman*; H. Nikfarjad

    2018-01-01

    Background and objectives: Sleep disorder is one of the main problems in children. Poor sleep quality can lead to adverse effects on their growth and development. Aromatherapy is a kind of method for improving sleep. In Iranian traditional medicine, inhaling Rosa damascena has been recommended for treating sleep disorder. Due to the side effects of chemical drugs and trend to alternative medicine due to less complication, the aim of this study was to investigate the effect of aromatherapy wit...

  17. Rosa canina Extracts Have Antiproliferative and Antioxidant Effects on Caco-2 Human Colon Cancer.

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    Sandra Jiménez

    Full Text Available The in vitro antiproliferative and antioxidant effects of different fractions of Rosa canina hips on human colon cancer cell lines (Caco-2 was studied. The compounds tested were total extract (fraction 1, vitamin C (fraction 2, neutral polyphenols (fraction 3 and acidic polyphenols (fraction 4. All the extracts showed high cytotoxicity after 72 h, both low and high concentrations. The flow cytometric analysis revealed that all the fractions produce disturbances in the cell cycle resulting in a concomitant cell death by an apoptotic pathway. Changes in the redox status of Caco-2 cells in response to Rosa canina hips were determined. Cells were exposed to hydrogen peroxide in presence of plant fractions and the production of Reactive Oxygen Species (ROS was significantly decreased. Therefore, our data demonstrate that rosehip extracts are a powerful antioxidant that produces an antiproliferative effect in Caco-2 cells. Therefore, these results predict a promising future for Rosa canina as a therapeutic agent. Thus, this natural plant could be an effective component of functional foods addressed towards colorectal carcinoma.

  18. E uma rosa se abre: a guerra e a flor na poesia de Drummond

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    Valéria Daiane Soares Rodrigues

    2013-08-01

    Full Text Available Carlos Drummond de Andrade, em A Rosa do Povo (1945 articula, por meio da palavra e da criação de um “eu” lírico habitante de um mundo em guerra, a construção de uma imagem, imbuída de teor histórico, do Brasil dos anos finais da 2ª guerra mundial. Os versos traduzem a experiência do Drummond gauche, angustiado pelos problemas do seu tempo, em que a individualidade advinda da fragmentação do homem moderno é marca do conflito entre o “eu” lírico e o mundo. O poeta descreve de forma melancólica a degeneração das coisas e dos homens, em meio à consciência paralisante e a falta de perspectivas. A força que emerge do símbolo da rosa respalda o acento poético do Itabirano: a destruição dos valores provocados pela morte e pelo derramar de sangue contrasta com o brotar da flor no asfalto.  Do chão forrado de cadáveres, emerge uma rosa, numa representação da resistência humana.

  19. Flora vascular y vegetación del humedal de Santa Rosa (Chancay, Lima

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    Dámaso W. Ramirez

    2011-05-01

    Full Text Available El presente trabajo muestra los resultados de un estudio de la flora y vegetación del humedal de Santa Rosa (Chancay, Lima realizado entre los años 2007 y 2009. La flora vascular estuvo conformada por 66 especies agrupadas en 57 géneros y 26 familias. Las Poaceae (16, Cyperaceae (6 y Asteraceae (6 fueron las familias más diversas y constituyeron el 42% de la flora total. En comparación con los principales humedales de Lima, Santa Rosa presenta un mayor número de especies (Pantanos de Villa (65, Paraíso (26 y Medio Mundo (16. El análisis de similitud florística muestra que Santa Rosa es más semejante a Los Pantanos de Villa (Lima. El Análisis de Correspondencia (AC determinó tres comunidades vegetales predominantes: a Comunidad de acuáticas flotantes, b Totoral y asociadas y c Vega Mixta. Se considera que los cambios en la estructura de las comunidades vegetales y el número de especies introducidas son indicadores del impacto antrópico al que está sometido este ecosistema. Se identifican las fuentes de impacto humano y se considera a la agricultura como la principal amenaza del humedal.

  20. Comparisons of ROSA-III and FIST BWR loss of coolant accident simulation tests

    International Nuclear Information System (INIS)

    Tasaka, Kanji; Suzuki, Mitsuhiro; Koizumi, Yasuo

    1985-10-01

    A common understanding and interpretation of BWR system response and the controlling phenomena in LOCA transients has been achieved through the evaluation and comparison of counterpart tests performed in the ROSA-III and FIST test facilities. These facilities, which are designed to simulate the thermal-hydraulic response of BWR systems, are operated respectively by the Japan Atomic Energy Research Institute (JAERI) and the General Electric Company. Comparison is made between three types of counterpart tests, each performed under similar tests conditions in the two facilities. They are large break, small break, and steamline break LOCA's. The system responses to these tests in each facility are quite similar. The sequence of events are similar, and the timing of these events are similar. Differences that do occur are due to minor differences in modeling objectives, facility scaling, and test conditions. Parallel channel flow interactions effects in the ROSA-III four channel (half length) core, although noticeable in the large break test, do not result in major differences with the single channel response in FIST. In the small break tests the timing of events is offset by the earlier ADS actuation in FIST. The steamline test responses are similar except there is no heatup in FIST, resulting from a different ECCS trip modeling. Overall comparisons between ROSA-III and FIST system responses in LOCA tests is very good. (author)

  1. The effect of aromatherapy with Rosa damascena essential oil on sleep quality in children

    Directory of Open Access Journals (Sweden)

    A.S. Keyhanmehr

    2018-01-01

    Full Text Available Background and objectives: Sleep disorder is one of the main problems in children. Poor sleep quality can lead to adverse effects on their growth and development. Aromatherapy is a kind of method for improving sleep. In Iranian traditional medicine, inhaling Rosa damascena has been recommended for treating sleep disorder. Due to the side effects of chemical drugs and trend to alternative medicine due to less complication, the aim of this study was to investigate the effect of aromatherapy with Rosa damascena essential oil on sleep quality in children. Methods: This study was an experimental before and after study that conducted in 30 children with sleep disorder. Children inhaled 5 drops of Rosa damascena essential oil on a cotton ball before sleep for 20 min (2 weeks. Before and after intervention, BEARS questionnaire was asked. Wilcoxon signed-rank test was used for comparisons by SPSS software. Results: The results of this study showed that resistance to sleep, difficulty waking in the morning, nightmare and waking up during the night in children decreased (p

  2. Post-test analysis of the ROSA/LSTF and PKL counterpart test

    Energy Technology Data Exchange (ETDEWEB)

    Carlos, S., E-mail: scarlos@iqn.upv.es [Departament d’Enginyeria Química i Nuclear, Universitat Politècnica de València, Camí de Vera, 14, València Spain (Spain); Querol, A., E-mail: anquevi@iqn.upv.es [Departament d’Enginyeria Química i Nuclear, Universitat Politècnica de València, Camí de Vera, 14, València Spain (Spain); Instituto de Seguridad Industrial, Radiofísica y Medioambiental, Universitat Politècnica de València, Camí de Vera, 14, València (Spain); Gallardo, S., E-mail: sergalbe@iqn.upv.es [Departament d’Enginyeria Química i Nuclear, Universitat Politècnica de València, Camí de Vera, 14, València Spain (Spain); Instituto de Seguridad Industrial, Radiofísica y Medioambiental, Universitat Politècnica de València, Camí de Vera, 14, València (Spain); Sanchez-Saez, F., E-mail: frasansa@etsii.upv.es [Departament d’Enginyeria Química i Nuclear, Universitat Politècnica de València, Camí de Vera, 14, València Spain (Spain); and others

    2016-02-15

    Highlights: • TRACE modelization for PKL and ROSA/LSTF installations. • Secondary-side depressurization as accident management action. • CET vs PCT relation. • Analysis of differences in the vessel models. - Abstract: Experimental facilities are scaled models of commercial nuclear power plants, and are of great importance to improve nuclear power plants safety. Thus, the results obtained in the experiments undertaken in such facilities are essential to develop and improve the models implemented in the thermal-hydraulic codes, which are used in safety analysis. The experiments and inter-comparisons of the simulated results are usually performed in the frame of international programmes in which different groups of several countries simulate the behaviour of the plant under the accidental conditions established, using different codes and models. The results obtained are compared and studied to improve the knowledge on codes performance and nuclear safety. Thus, the Nuclear Energy Agency (NEA), in the nuclear safety work area, auspices several programmes which involve experiments in different experimental facilities. Among the experiments proposed in NEA programmes, one on them consisted of performing a counterpart test between ROSA/LSTF and PKL facilities, with the main objective of determining the effectiveness of late accident management actions in a small break loss of coolant accident (SBLOCA). This study was proposed as a result of the conclusion obtained by the NEA Working Group on the Analysis and Management of Accidents, which analyzed different installations and observed differences in the measurements of core exit temperature (CET) and maximum peak cladding temperature (PCT). In particular, the transient consists of a small break loss of coolant accident (SBLOCA) in a hot leg with additional failure of safety systems but with accident management measures (AM), consisting of a fast secondary-side depressurization, activated by the CET. The paper

  3. Post-test analysis of the ROSA/LSTF and PKL counterpart test

    International Nuclear Information System (INIS)

    Carlos, S.; Querol, A.; Gallardo, S.; Sanchez-Saez, F.

    2016-01-01

    Highlights: • TRACE modelization for PKL and ROSA/LSTF installations. • Secondary-side depressurization as accident management action. • CET vs PCT relation. • Analysis of differences in the vessel models. - Abstract: Experimental facilities are scaled models of commercial nuclear power plants, and are of great importance to improve nuclear power plants safety. Thus, the results obtained in the experiments undertaken in such facilities are essential to develop and improve the models implemented in the thermal-hydraulic codes, which are used in safety analysis. The experiments and inter-comparisons of the simulated results are usually performed in the frame of international programmes in which different groups of several countries simulate the behaviour of the plant under the accidental conditions established, using different codes and models. The results obtained are compared and studied to improve the knowledge on codes performance and nuclear safety. Thus, the Nuclear Energy Agency (NEA), in the nuclear safety work area, auspices several programmes which involve experiments in different experimental facilities. Among the experiments proposed in NEA programmes, one on them consisted of performing a counterpart test between ROSA/LSTF and PKL facilities, with the main objective of determining the effectiveness of late accident management actions in a small break loss of coolant accident (SBLOCA). This study was proposed as a result of the conclusion obtained by the NEA Working Group on the Analysis and Management of Accidents, which analyzed different installations and observed differences in the measurements of core exit temperature (CET) and maximum peak cladding temperature (PCT). In particular, the transient consists of a small break loss of coolant accident (SBLOCA) in a hot leg with additional failure of safety systems but with accident management measures (AM), consisting of a fast secondary-side depressurization, activated by the CET. The paper

  4. Compostos bioativos em polpas de mangas 'rosa' e 'espada' submetidas ao branqueamento e congelamento

    Directory of Open Access Journals (Sweden)

    Lucimara Piauí Soares

    2013-06-01

    Full Text Available A manga (Mangifera indica L. constitui uma importante fonte de compostos bioativos, dentre os quais se destacam os carotenoides e a vitamina C, que contribuem para a promoção da saúde. Vários métodos podem ser utilizados para conservação de alimentos a fim de aproveitar melhor os frutos durante a safra e permitir seu armazenamento fora de época de produção. No entanto, podem alterar a qualidade nutricional do alimento. Dessa forma, sendo o branqueamento e o congelamento métodos que são utilizados no processamento industrial para conservação de polpas de frutas, objetivou-se, neste trabalho, analisar a influência do branqueamento e do congelamento sobre os teores de carotenoides totais e ácido ascórbico em mangas 'Rosa' e 'Espada' em relação ao tempo de armazenamento. As mangas no estádio maduro, após serem colhidas, lavadas, cortadas e despolpadas, foram submetidas ao congelamento (-18ºC ou branqueamento por imersão em água (75ºC, por 3 minutos ou branqueamento por vapor em água fervente, por 3 minutos. Em seguida, foram realizadas as determinações bioquímicas dos teores de carotenoides totais e ácido ascórbico no tempo zero e após 20; 40 e 60 dias de armazenamento sob congelamento. Após análise dos resultados obtidos, conclui-se que, na forma in natura (tempo zero, as variedades Rosa e Espada apresentam os máximos teores de ácido ascórbico, porém o uso do branqueamento com vapor e o congelamento após 60 dias de armazenamento provocam perdas de, respectivamente, 53% ('Rosa' e 35% ('Espada'; 72% ('Rosa' e 60% ('Espada' destes compostos. No branqueamento por imersão em água, há perda completa do ácido ascórbico nas polpas das variedades Rosa e Espada. Em relação aos carotenoides totais, os valores foram semelhantes em polpas branqueadas a vapor e sem branqueamento no tempo zero,com tendência de redução durante o longo da armazenagem.

  5. Heavy metal accumulation imparts structural differences in fragrant Rosa species irrigated with marginal quality water.

    Science.gov (United States)

    Ahsan, Muhammad; Younis, Adnan; Jaskani, Muhammad Jafar; Tufail, Aasma; Riaz, Atif; Schwinghamer, Timothy; Tariq, Usman; Nawaz, Fahim

    2018-09-15

    Wastewater is an alternative to traditional sources of renewable irrigation water in agriculture, particularly in water-scarce regions. However, the possible risks due to heavy metals accumulation in plant tissues are often overlooked by producers. The present study aimed to identify heavy metals-induced structural modifications to roots of scented Rosa species that were irrigated with water of marginal quality. The chemical and mineral contents from the experimental irrigation canal water (control) and treated wastewater were below the limits recommended by the Pakistan Environmental Protection Agency (Pak-EPA) for medicinal plants. The experimentally untreated wastewater contained electrical conductivity (EC), chemical oxygen demand (COD), biological oxygen demand (BOD), and heavy metals (Co, Cu, Cd, Pb) that were above the recommended limits. The responses by wastewater-treated Rosa species (Rosa damascena, R. bourboniana, R. Gruss-an-Teplitz, and R. centifolia) were evaluated. The experimental data revealed that treated wastewater significantly increased the thickness of collenchyma (cortex and pith) and parenchyma tissues (vascular bundle, xylem, and phloem) of R. Gruss-an-Teplitz. Root dermal tissues (epidermis) of R. bourboniana also responded to treated wastewater. R. damascena and R. centifolia were the least affected species, under the experimental irrigation conditions. Collenchyma and dermal tissues were thicker in R. damascena and R. Gruss-an-Teplitz under untreated wastewater conditions. In parenchyma tissues, vascular bundles were thicker in R. damascena in untreated wastewater conditions, while the xylem and phloem of R. Gruss-an-Teplitz were thicker where treated wastewater was applied. In tissues other than the vascular bundle, the differences in anatomical metrics due to the experimental irrigation treatments were greater during the second year of the experiment than in the first year. The contents of metals other than chromium in the roots and

  6. Anonymous voting for multi-dimensional CV quantum system

    International Nuclear Information System (INIS)

    Shi Rong-Hua; Xiao Yi; Shi Jin-Jing; Guo Ying; Lee, Moon-Ho

    2016-01-01

    We investigate the design of anonymous voting protocols, CV-based binary-valued ballot and CV-based multi-valued ballot with continuous variables (CV) in a multi-dimensional quantum cryptosystem to ensure the security of voting procedure and data privacy. The quantum entangled states are employed in the continuous variable quantum system to carry the voting information and assist information transmission, which takes the advantage of the GHZ-like states in terms of improving the utilization of quantum states by decreasing the number of required quantum states. It provides a potential approach to achieve the efficient quantum anonymous voting with high transmission security, especially in large-scale votes. (paper)

  7. Instant OpenCV for iOS

    CERN Document Server

    Kornyakov, Kirill

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. This book uses a very practical approach, with each recipe and their associated sample projects or examples focusing on a particular aspect of the technology.This book is intended for OpenCV developers who are interested in porting their applications to the iOS platform. Basic experience with OpenCV, computer vision, Objective C, and other iOS tools is encouraged.

  8. Verification of a TRACE EPRTM model on the basis of a scaling calculation of an SBLOCA ROSA test

    International Nuclear Information System (INIS)

    Freixa, J.; Manera, A.

    2011-01-01

    Research highlights: → Verification of a TRACE input deck for the EPR TM generation III PWR. → Scaling simulation of an SBLOCA experiment of the integral test facility ROSA/LSTF. → The EPR TM model was compared with the TRACE results of the ROSA/LSTF model. - Abstract: In cooperation with the Finnish Radiation and Nuclear Safety Authority (STUK), a project has been launched at the Paul Scherrer Institute (PSI) aimed at performing safety evaluations of the Olkiluoto-3 nuclear power plant (NPP), the first EPR TM , a generation III pressurizer water reactor (PWR); with particular emphasis on small-and large-break loss-of-coolant-accidents (SB/LB-LOCAs) and main steam-line breaks. As a first step of this work, the best estimate system code TRACE has been used to develop a model of Olkiluoto-3. In order to test the nodalization, a scaling calculation from the rig of safety assessment (ROSA) test facility has been performed. The ROSA large scale test facility (LSTF) was built to simulate Westinghouse design pressurized water reactors (PWR) with a four-loop configuration. Even though there are differences between the EPR TM and the Westinghouse designs, the number of similarities is large enough to carry out scaling calculations on SBLOCA and LOCA cases from the ROSA facility; as a matter of fact, the main differences are located in the secondary side. Test 6-1 of the ROSA 1 programme, an SBLOCA with the break situated in the upper head of the reactor pressure vessel (RPV), was of special interest since a very good agreement with the experiment was obtained with a TRACE input deck. In order to perform such scaling calculation, the set-points of the secondary relief and safety valves in the EPR TM nodalization had to be changed to those used in the ROSA facility, the break size and the core power had to be scaled by a factor of 60 (according to the core power and core volume) and the pumps coast down had to be adapted to the ones of the test. The calculation showed

  9. Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat

    Science.gov (United States)

    Gauthaman, Karunakaran K; Saleem, Mohamed TS; Thanislas, Peter T; Prabhu, Vinoth V; Krishnamoorthy, Karthikeyan K; Devaraj, Niranjali S; Somasundaram, Jayaprakash S

    2006-01-01

    Background The present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, particularly in terms of its antioxidant effects. Methods The medicinal values of the flowers of Hibiscus rosa sinensis (Chinese rose) have been mentioned in ancient literature as useful in disorders of the heart. Dried pulverized flower of Hibiscus rosa sinensis was administered orally to Wistar albino rats (150–200 gms) in three different doses [125, 250 and 500 mg/kg in 2% carboxy methyl cellulose (CMC)], 6 days per week for 4 weeks. Thereafter, rats were sacrificed; either for the determination of baseline changes in cardiac endogenous antioxidants [superoxide dismutase, reduced glutathione and catalase] or the hearts were subjected to isoproterenol induced myocardial necrosis. Results There was significant increase in the baseline contents of thiobarbituric acid reactive substances (TBARS) [a measure of lipid per oxidation] with both doses of Hibiscus Rosa sinensis. In the 250 mg/kg treated group, there was significant increase in superoxide dismutase, reduced glutathione, and catalase levels but not in the 125 and 500 mg/kg treated groups. Significant rise in myocardial thiobarbituric acid reactive substances and loss of superoxide dismutase, catalase and reduced glutathione (suggestive of increased oxidative stress) occurred in the vehicle treated hearts subjected to in vivo myocardial ischemic reperfusion injury. Conclusion It may be concluded that flower of Hibiscus rosa sinensis (250 mg/kg) augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from isoproterenol induced myocardial injury. PMID:16987414

  10. Ensacamento de frutos de pereira cv. Housui Bagging of nashi pear cv. Housui

    Directory of Open Access Journals (Sweden)

    Ivan Dagoberto Faoro

    2004-04-01

    Full Text Available Foi avaliada a qualidade de frutos de pêra japonesa cv. Housui (Pyrus pyrifolia var. culta ensacados com diferentes tipos de sacos de papel e em duas épocas: 34 e 83 dias após a florada. O ensacamento não influenciou na firmeza, no teor de sólidos solúveis totais e no peso médio dos frutos. Sacos vermelhos não induziram aumento do pH da polpa, mas o uso de sacos duplos, sacos marrons, sacos de papel kraft marrons e sacos de pipoca brancos aumentaram significativamente o pH. Ao buscar-se maior precocidade de colheita, os melhores resultados foram obtidos com o uso de sacos pequenos de papel manteiga aos 34 dias após a floração e o uso de sacos grandes duplos ou sacos grandes marrons, 83 dias após a plena floração. O ensacamento 34 dias após a plena floração, com sacos grandes de papel duplo de cor marrom ou sacos de papel kraft marrons, ou ainda o uso de sacos pequenos parafinados transparentes de papel manteiga, aos 34 dias, seguidos pela colocação, aos 83 dias, dos dois tipos de sacos grandes citados anteriormente, resultaram em frutos de melhor qualidade externa (película de coloração homogênea e mais clara, lisa e com lenticelas pouco salientes. O uso de sacos vermelhos de papel manteiga e de sacos de pipoca brancos, com ou sem ensacamento prévio com sacos pequenos de papel manteiga parafinado, não resultaram em melhoria substancial da qualidade externa do fruto.The fruit quality of Nashi pears cv. Housui (Pyrus pyrifolia var. culta have been evaluated after bagging with different paper bags at two bagging dates: 34 and 83 days after full bloom. The bagging did not affect the fruit firmness, the total soluble solids and the average fruit weight. Red bags did not affect the pH of the fruit flesh. However the fruit bagged with double bags of brown color, brown kraft paper and white popcorn paper increased the pH. In order to harvest precociously, the use of small transparent paraffin paper bags 34 days after full bloom

  11. Pressurized fluid extraction of essential oil from Lavandula hybrida using a modified supercritical fluid extractor and a central composite design for optimization.

    Science.gov (United States)

    Kamali, Hossein; Jalilvand, Mohammad Reza; Aminimoghadamfarouj, Noushin

    2012-06-01

    Essential oil components were extracted from lavandin (Lavandula hybrida) flowers using pressurized fluid extraction. A central composite design was used to optimize the effective extraction variables. The chemical composition of extracted samples was analyzed by a gas chromatograph-flame ionization detector column. For achieving 100% extraction yield, the temperature, pressure, extraction time, and the solvent flow rate were adjusted at 90.6°C, 63 bar, 30.4 min, and 0.2 mL/min, respectively. The results showed that pressurized fluid extraction is a practical technique for separation of constituents such as 1,8-cineole (8.1%), linalool (34.1%), linalyl acetate (30.5%), and camphor (7.3%) from lavandin to be applied in the food, fragrance, pharmaceutical, and natural biocides industries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Characterisation of the nucleolar organising regions during the cell cycle in two varieties of Petunia hybrida as visualised by fluorescence in situ hybridisation and silver staining.

    Science.gov (United States)

    Montijn, M B; ten Hoopen, R; Fransz, P F; Oud, J L; Nanninga, N

    1998-05-01

    The cell cycle-dependent spatial position, morphology and activity of the four nucleolar organising regions (NORs) of the Petunia hybrida cultivar Mitchell and the inbred line V26 have been analysed. Application of the silver staining technique and fluorescence in situ hybridisation on fixed root-tip material revealed that these interspecific hybrids possess four NORs of which only those of chromosome 2 are active during interphase, which implies that the NOR activity is not of parental origin. However, at the end of mitosis, activity of all NOR regions could be detected, suggesting that the high demand for ribosomes at this stage of the cell cycle requires temporal activity of all NORs. Using actin DNA probes as markers in fluorescence in situ hybridisation experiments enabled the identification of the individual petunia chromosomes.

  13. Plant regeneration of Brassica oleracea subsp. italica (Broccoli) CV ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-06-03

    Jun 3, 2009 ... Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul. Ehsan, Malaysia. Accepted 20 March, 2009. Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp.italica) cv. Green Marvel.

  14. Biology of Maconellicoccus hirsutus (Hemiptera: Pseudococcidae on Hibiscus rosa-sinensis = Biologia de Maconellicoccus hirsutus (Hemiptera: Pseudococcidae em Hibiscus rosa-sinensis

    Directory of Open Access Journals (Sweden)

    Marcelo Negrini

    2017-10-01

    Full Text Available Maconellicoccus hirsutus Green, 1908 (Hemiptera: Pseudococcidae is an introduced pest that threatens fruit and ornamental plant production in Brazil. Reported in Brazil for the first time in 2010, in the state of Roraima, M. hirsutus, has spread rapidly to other regions of the country. The objective of this study was to investigate the biology of M. hirsutus on Hibiscus rosa-sinensis L. (Malvaceae leaves in laboratory conditions with climatic parameters similar to those of the Brazilian North and Northeast (27 ± 1 °C, 60 ± 10% relative humidity and 12 hours light and 12 hours dark. Eighty M. hirsutus nymphs were individualized on rooted H. rosa-sinensis leaves and their survival, mortality, sex ratio, and egg numbers per female emerged were recorded. Maconellicoccus hirsutus can have up to nine generations per year, taking 6.5 days to double its population size. The female numbers of this pest are about three times higher than those for its male counterparts, with 98 eggs per female and 97% viability. The survival curve of the species is type I, that is, mortality rates are higher in adulthood. Maconellicoccus hirsutus, especially, a pest of fruit trees and ornamental plants, presents great potential for population growth in Brazilian tropical conditions. = Maconellicoccus hirsutus Green, 1908 (Hemiptera: Pseudococcidae é uma praga introduzida que ameaça a produção de frutas e plantas ornamentais no Brasil. Registrada no Brasil pela primeira vez em 2010, no estado de Roraima, M. hirsutus se espalhou rapidamente para outras regiões do país. O objetivo deste estudo foi investigar a biologia de M. hirsutus em folhas de Hibiscus rosa-sinensis L. (Malvaceae, em condições de laboratório com parâmetros climáticos semelhantes aos das regiões Norte e Nordeste do Brasil (27 ± 1 °C, 60 ± 10 % de umidade relativa e 12 h de luz e 12 h de escuro. Oitenta ninfas de M. hirsutus foram individualizadas em folhas enraizadas de H. rosa-sinensis e

  15. Methodology for the design of Santa Rosa de Cabal sanitary landfill, Risaralda; Metodologia para el diseno del relleno sanitario de Santa Rosa de Cabal, Risaralda

    Energy Technology Data Exchange (ETDEWEB)

    Tabares, J; Orozco, J

    1992-07-01

    In 1987 the Regional Autonomous Corporation of Risaralda, CARDER and the Risaralda Government, they signed a cooperation agreement, in order to endowing from sanitary landfill to the municipalities of the department. In the mark of this agreement it was carried out the design of Santa Rosa's sanitary landfill, that with near 50.000 inhabitants it is constituted in the third city of the department. This city generates some 25 tons/day of garbage that at the present time are heady directly to the waters of San Eugenio River. The present work contains the most important methodological aspects in the design of the sanitary landfill and some comments about the approaches ideal Vs real approaches of selection of places.

  16. Intertextual Sexual Politics: Illness and Desire in Enrique Gomez Carrillo's "Del amor", "del dolor y del vicio" and Aurora Caceres's "La rosa muerta"

    Science.gov (United States)

    LaGreca, Nancy

    2012-01-01

    This study explores the intertextuality between Aurora Caceres's "La rosa muerta" (1914) and the novel "Del amor, del dolor y del vicio" (1898) by her ex-husband, Enrique Gomez Carrillo. Caceres strategically mentions Gomez Carrillo's novel in "La rosa muerta" to invite a reading of her work in dialogue with his. Both narratives follow the sexual…

  17. Erzincan ve Çevresinde Yayılış Gösteren Doğal Gül ( Rosa L. Taksonları

    Directory of Open Access Journals (Sweden)

    Mustafa KORKMAZ

    2014-07-01

    Full Text Available Roses (Rosa L. is known as the genus of fragrant, nice-looking plants of Rosaceae family. Rosa which has a history older than the history of humanity, due to the alluring beauty and nice- smell were cultivated and used throughout the ages

  18. Tandemly arranged chalcone synthase A genes contribute to the spatially regulated expression of siRNA and the natural bicolor floral phenotype in Petunia hybrida.

    Science.gov (United States)

    Morita, Yasumasa; Saito, Ryoko; Ban, Yusuke; Tanikawa, Natsu; Kuchitsu, Kazuyuki; Ando, Toshio; Yoshikawa, Manabu; Habu, Yoshiki; Ozeki, Yoshihiro; Nakayama, Masayoshi

    2012-06-01

    The natural bicolor floral traits of the horticultural petunia (Petunia hybrida) cultivars Picotee and Star are caused by the spatial repression of the chalcone synthase A (CHS-A) gene, which encodes an anthocyanin biosynthetic enzyme. Here we show that Picotee and Star petunias carry the same short interfering RNA (siRNA)-producing locus, consisting of two intact CHS-A copies, PhCHS-A1 and PhCHS-A2, in a tandem head-to-tail orientation. The precursor CHS mRNAs are transcribed from the two CHS-A copies throughout the bicolored petals, but the mature CHS mRNAs are not found in the white tissues. An analysis of small RNAs revealed the accumulation of siRNAs of 21 nucleotides that originated from the exon 2 region of both CHS-A copies. This accumulation is closely correlated with the disappearance of the CHS mRNAs, indicating that the bicolor floral phenotype is caused by the spatially regulated post-transcriptional silencing of both CHS-A genes. Linkage between the tandemly arranged CHS-A allele and the bicolor floral trait indicates that the CHS-A allele is a necessary factor to confer the trait. We suppose that the spatially regulated production of siRNAs in Picotee and Star flowers is triggered by another putative regulatory locus, and that the silencing mechanism in this case may be different from other known mechanisms of post-transcriptional gene silencing in plants. A sequence analysis of wild Petunia species indicated that these tandem CHS-A genes originated from Petunia integrifolia and/or Petunia inflata, the parental species of P. hybrida, as a result of a chromosomal rearrangement rather than a gene duplication event. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.

  19. Micromycetes on climbing roses leaves (Rosa L. in the Botanic Garden of the Jagiellonian University in Cracow

    Directory of Open Access Journals (Sweden)

    Maria Kowalik

    2015-08-01

    Full Text Available Micromycetes inhabiting the leaves of 20 cultivars of climbing roses (Rosa L., grown in Botanic Garden of the Jagiellonian University in Cracow was investigated in the three successive years of research. Sixty-five taxa of of micromycetes was recorded with a few species dominating: Alternaria alternata, Epicoccum nigrum, Pestalotia rosae, Penicillium brevicompactum and Sordaria fimicola, accompanied by various other microfungi. A high abundance of rose black spot caused by Diplocarpon rosae was also observed. The affected leaves revealed advancing necrosis, substantially enhancing at the end of the growing season. Defoliation took place from June to October. Micromycetes inhabiting the leaves of climbing roses in Botanic Garden of the Jagiellonian University in Cracow considerably deteriorated the decorative aspect of the plants.

  20. Population Growth Parameters of Rose Aphid, Macrosiphum rosae (Hemiptera: Aphididae) on Different Rose Cultivars.

    Science.gov (United States)

    Golizadeh, A; Jafari-Behi, V; Razmjou, J; Naseri, B; Hassanpour, M

    2017-02-01

    The rose aphid, Macrosiphum rosae (L.), is one of the most important pests on rose plants (Rosa spp.) with a worldwide distribution. As resistance indices, the development, survivorship, and reproduction of this aphid were evaluated on 10 rose cultivars, including Bella Vita, Cool Water, Dolce Vita, Maroussia, Orange Juice, Pinkpromise, Roulette, Tea, Valentine, and Persian Yellow in laboratory at 25 ± 1°C, 65 ± 5% relative humidity, and photoperiod of 16:8 (L/D) h. Rose aphid successfully survived on all 10 rose cultivars, although mortality rate was higher on Tea and Bella Vita. The number of offspring per female differed significantly among the tested rose cultivars, and ranged from 9.2 on Tea to 38.7 nymphs on Orange Juice. Population growth parameters were significantly affected by rose cultivars. The longest mean generation time (T) was observed on Bella Vita (14.8 days) and Tea (14.7 days) and the shortest on Orange Juice (10.0 days). The net reproductive rate (R 0 ) ranged from 6.9 on Tea to 33.2 nymphs on Orange Juice cultivar. Correspondingly, the highest value of intrinsic rate of increase (r m ) was observed on Orange Juice (0.348 day -1 ) and lower values on Tea (0.131 day -1 ) followed by Bella Vita (0.154 day -1 ). Cluster analysis of all the measured parameters of rose aphid on different rose cultivars revealed that Tea and Bella Vita were relatively resistant to M. rosae. These findings could be useful in developing an integrated pest management (IPM) program for this aphid in urbanized areas and commercial rose potting.

  1. DIVERSIDAD GENÉTICA DE Peronospora sparsa (PERONOSPORACEAE EN CULTIVOS DE ROSA DE COLOMBIA

    Directory of Open Access Journals (Sweden)

    MARILUZ AYALA-VÁSQUEZ

    2008-01-01

    Full Text Available El mildeo velloso de la rosa es la enfermedad más limitante de este cultivo en Colombia. Aunque algunos trabajos de investigación se han adelantado recientemente sobre la epidemiología de esta enfermedad, uno de los campos menos estudiados es el nivel de variabilidad de su agente causal. El conocimiento de este aspecto es fundamental para definir las estrategias de manejo, especialmente aquellas relacionadas con la resistencia genética y el control químico. Los objetivos de esta investigación fueron confirmar la identidad taxonómica del agente causal del mildeo velloso de la rosa y profundizar en el conocimiento de su estructura poblacional en Colombia. Para esto se colectaron 34 aislamientos del pseudohongo en cultivos de rosas ubicados en Antioquia y la sabana de Bogotá y se extrajo su ADN, para proceder a la amplificación mediante PCR de la región ITS del ADNr utilizando los primers especieespecíficos PS3 y PS1. Dichos productos se emplearon tanto para su secuenciación directa como para la evaluación de variabilidad genética mediante la técnica PCRRFLP, resultados que se complementaron con la utilización de los marcadores RAPD y RAMS. La presencia de un amplicón de aproximadamente 700 pb que compartió un porcentaje de identidad del 100% con secuencias depositadas en el GenBank, permitió confirmar a los aislamientos como pertenecientes a Peronospora sparsa, mientras que el análisis de RFLP, RAPD y RAMS indicó un muy bajo nivel de variabilidad entre todos los aislamientos, concluyéndose que la población de P. sparsa en Colombia es predominantemente clonal

  2. DIAGNÓSTICO SEROLÓGICO (ROSA DE BENGALA) Y MOLECULAR (PCR) DE BRUCELOSIS EN HUMANO

    OpenAIRE

    Orly Fernando Cevallos Falquez; Aldelmo Rodríguez Grefa; Ariel Escobar Troya; Camilo Mestanza Uquillas; Diego Romero Garaicoa; Fabricio Canchignia Martínez; Jaime Fabian Vera Chang; Jonathan Mariscal Álvarez; Ketty Cobeña Rosado; Luís Ramos Gavilanes; Maria Lorena Cadme; Mercedes Susana Carranza Patiño; Silvia Gicela Saucedo Aguiar; Ximena Reyes Chancay

    2010-01-01

    El objetivo de esta investigación fue: Determinar y comparar la presencia de Brucelosis del personal que labora en los camales de los cantones, Buena Fé, Quevedo, El Empalme y Pichincha, utilizando la prueba serológica Rosa de Bengala (RB) y la técnica molecular (PCR). Se utilizó como fuente de ADN y anticuerpos sangre periférica. De un total de 115 muestras de sangre recolectadas al personal que labora en los camales como faenadores y operadores, 54 (47%) y 15 (13%) fueron positivas con RB y...

  3. PORTUGUESE LITERATURE FOR CHILDREN: AQUILINO RIBEIRO AND MATILDE ROSA ARAÚJO

    Directory of Open Access Journals (Sweden)

    Luci Ruas

    2014-11-01

    Full Text Available This work shows that, in the twentieth century, specifically in its second half, many of the texts produced for children already showed clear concern in separating itself from the receiver, so that value is given to how they are built, that is, so that from the writing scripture is made, and from text, literature. For this purpose, we start by presenting the concerns of Antero de Quental and Eça de Queiroz, and then approaching the literary work of Aquilino Ribeiro, specifically O romance da raposa, following with the approach present in the literary work of Matilde Rosa Araújo. 

  4. DIVERSIDAD GENÉTICA DE Peronospora sparsa (PERONOSPORACEAE) EN CULTIVOS DE ROSA DE COLOMBIA

    OpenAIRE

    MARILUZ AYALA-VÁSQUEZ; LUZ EDITH ARGEL-ROLDAN; MAURICIO MARÍN-MONTOYA

    2008-01-01

    El mildeo velloso de la rosa es la enfermedad más limitante de este cultivo en Colombia. Aunque algunos trabajos de investigación se han adelantado recientemente sobre la epidemiología de esta enfermedad, uno de los campos menos estudiados es el nivel de variabilidad de su agente causal. El conocimiento de este aspecto es fundamental para definir las estrategias de manejo, especialmente aquellas relacionadas con la resistencia genética y el control químico. Los objetivos de esta investigación...

  5. DIVERSIDAD GENÉTICA DE Peronospora sparsa (PERONOSPORACEAE) EN CULTIVOS DE ROSA DE COLOMBIA

    OpenAIRE

    MARILUZ AYALA-VÁSQUEZ; LUZ EDITH ARGEL-ROLDAN; SONIA JARAMILLO-VILLEGAS; MAURICIO MARÍN-MONTOYA

    2008-01-01

    El mildeo velloso de la rosa es la enfermedad más limitante de este cultivo en Co- lombia. Aunque algunos trabajos de investigación se han adelantado recientemente sobre la epidemiología de esta enfermedad, uno de los campos menos estudiados es el nivel de variabilidad de su agente causal. El conocimiento de este aspecto es fun- damental para definir las estrategias de manejo, especialmente aquellas relacionadas con la resistencia genética y el control químico. Los objetivos de esta investiga...

  6. Estrutura e significado em "Uma rosa para Emily", de William Faulkner

    OpenAIRE

    Daghlian,Carlos

    2004-01-01

    Trata-se de uma análise do consagrado conto "Uma Rosa para Emily", de William Faulkner, voltada para alguns dos principais aspectos de sua estrutura. Após considerarmos o enredo, discutimos a construção das personagens, com destaque para a protagonista, fazendo um levantamento e comentários sobre possíveis fontes de inspiração, destacando, entre outras, aspectos da biografia da poeta Emily Dickinson, a ficção e a poesia de E. A. Poe, romances de Charles Dickens e Henry James, o conto de Sherw...

  7. The Samba Controversy between Noel Rosa and Wilson Batista: Intertextuality and the Meanders of Composition

    Directory of Open Access Journals (Sweden)

    Leandro Moreira da Luz

    2015-07-01

    Full Text Available The purpose of this paper is to analyze the meanders of composition and their intertextuality in the samba controversy between Noel Rosa and Wilson Batista. We observe dialogism in the verbal and musical interaction between the samba writers and several texts of the 1930s. When ears are in tune with the "samba controversy," one observes that the compositions reveal inexorable tensions between the festive world of the "malandro" and the limits of the reality of the time. Accordingly, based on studies that address this theme, we aim to present an insight into this musical "duel," which is not restricted to purely aesthetics issues, but certainly disseminated in society.

  8. Sombras da Escuta - Guimarães Rosa criador de livros e textos

    Directory of Open Access Journals (Sweden)

    Mônica Gama

    2008-10-01

    Full Text Available Nous essayerons ici une lecture de l'historicité de la pratique de lecture proposée par Guimarães Rosa, qui même en étant un auteur connu pour sa capacité fabulation, il est aussi remarquable par laisser apparaître dans sa fiction la préoccupation par l'importance de la matérialité du livre et avec les processus d’appréhension de la lecture.

  9. Atividade fungitóxica de extratos vegetais e produtos comerciais contra Diplocarpon rosae

    Directory of Open Access Journals (Sweden)

    Janaina Miyashiro Simon

    Full Text Available RESUMO O objetivo deste trabalho foi verificar o potencial de extratos oriundos de plantas medicinais (Rosmarinus officinalis L., Equisetum arvense L. e Moringa oleifera Lam. e produtos comerciais (a base de extratos vegetais, óleos vegetais e fosfito de potássio no controle in vitro de Diplocarpon rosae. Avaliou-se o efeito dos tratamentos sobre o fitopatógeno pelo índice de velocidade de crescimento micelial, inibição do crescimento micelial, inibição da esporulação, índice de esporulação, síntese de proteínas, a atividade específica de peroxidase e catalase no micélio do fitopatógeno, utilizando extrato bruto aquoso (EBA de folhas de R. officinalis (1%; EBA de caules de E. arvense (1%; EBA de sementes de M. oleifera (1%; produto comercial a base de extratos vegetais fermentados (0,01%; produto comercial a base de fosfito de potássio (0,01%; produto comercial a base de óleos vegetais (0,01%; produto a base de massa cítrica (0,01%; fungicida a base de Tiofanato metílico (0,007%; adjuvante (0,0025% BDA e BD. Pelos resultados observou-se maior redução do crescimento de D. rosae quando na presença do produto comercial a base de óleos vegetais. O EBA de E. arvense e o produto comercial a base de extratos vegetais fermentados apresentaram efeito anti-esporulante. Os tratamentos com EBA de R. officinalis, EBA de E. arvense, EBA de M. oleifera, produto comercial a base de óleos vegetais e produto a base de massa cítrica aumentaram a síntese de proteínas no micélio D.rosae. Houve aumento da atividade específica das enzimas peroxidase e catalase no micélio quando na presença do EBA de R. officinalis e produto comercial a base de fosfito de potássio, respectivamente. O EBA de E. arvense, e os produtos comerciais a base de óleos vegetais e a base de extratos vegetais fermentados, apresentaram resultados promissores no controle do fitopatógeno D. rosae.

  10. GUIMARÃES ROSA: UMA REFLEXÃO SOBRE A QUESTÃO DA IDENTIDADE NACIONAL

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Viana Lyra

    2016-01-01

    Full Text Available A partir da discussão levantada por Guimarães Rosa no texto Pé-Duro, Chapéu-de-Couro, sobre a busca dos fundamentos da nacionalidade, o artigo enfoca a reflexão desenvolvida sobre os fundamentos da identidade nacional, analisando o tempo histórico correspondente e ressaltando o empenho do autor pela valorização das raízes sertanejas na constituição da nacionalidade brasileira. Palavras-chave: Nacionalidade, Raízes sertanejas, Identidade nacional

  11. A feeling for Systems Development Work - Design of the ROSA project

    DEFF Research Database (Denmark)

    Bødker, Susanne; Greenbaum, Joan

    1988-01-01

    This article is based on the design of a research project that will look at intuition, learning processes, language and roles in the development of computer systems. The research project, called ROSA (a Danish acronym for Roles and Cooperation in Systems Development) grew out of our interest...... in the informal working practices among systems developers, because it is these informal working relationships that are most often overlooked in research about computer science methods and tools. The project applies a gender perspective to look at the informal work relations of systems developers. The concept...

  12. Studies on the antifertility potentiality of Hibiscus rosa sinensis. Parts of medicinal value; selection of species and seasonal variations.

    Science.gov (United States)

    Kholkute, S D; Mudgal, V; Udupa, K N

    1977-02-01

    The postcoital antifertility properties of benzene hot extracts of Hibiscus rosa sinensis flowers, leaves, and stembarks, collected during the winter, spring, rainy, and summer seasons, were investigated in female rats. Only extracts from the flowers of the plant were 100% effective in preventing pregnancy. Those flowers collected during the winter showed the greatest potency, followed by those collected in the spring, rainy season, and summer, in decreasing order. Benzene extracts of flowers collected from Hibiscus mutabilis, Hibiscus schizopetalus, and Malvasicus grandiflorus, plants resembling Hibiscus rosa sinensis in petaloid structure, did not markedly affect pregnancy.

  13. Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa canina L.).

    Science.gov (United States)

    Saaby, Lasse; Jäger, Anna Katharina; Moesby, Lise; Hansen, Erik Wind; Christensen, Søren Brøgger

    2011-02-01

    A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.

  14. Hibiscus rosa- sinensis leaf extract as coagulant aid in leachate treatment

    Science.gov (United States)

    Awang, Nik Azimatolakma; Aziz, Hamidi Abdul

    2012-12-01

    Hibiscus rosa- sinensis is a biodegradable material that has remained untested for flocculating properties. The objective of this study is to examine the efficiency of coagulation-flocculation processes for the removal of color, iron (Fe3+), suspended solids, turbidity and ammonia nitrogen(NH3-N), from landfill leachate using 4,000 mg/L alum in conjunction with H. rosa- sinensis leaf extract (HBaqs). Hydroxyl (O-H) and (carboxyl) C=O functional groups along the HBaqs chain help to indulge flocculating efficiency of HBaqs via bridging. The experiments confirm the positive coagulation properties of HBaqs. The Fe3+ removal rate using 4,000 mg/L alum as sole coagulant was approximately 60 %, and increased to 100 % when 4,000 mg/L alum was mixed with 500 mg/L HBaqs. By mixing, 4,000 mg/L alum with 100-500 mg/L HBaqs, 72 % of SS was removed as compared with only 45 % reduction using 4,000 mg/L alum as sole coagulant.

  15. Experimental analysis of the power curve sensitivity test series at ROSA-III

    International Nuclear Information System (INIS)

    Koizumi, Y.; Iriko, M.; Yonomoto, T.; Tasaka, K.

    1985-01-01

    The rig of safety assessment (ROSA)-III facility is a volumetrically scaled (1/424) boiling water reactor (BWR/6) system with an electrically heated core designed for integral LOCA and ECCS tests. Seven recirculation pump suction line break LOCA experiments were conducted at the ROSA-III facility in order to examine the effect of the initial stored heat of a fuel rod on the peak cladding temperature (PCT). The break size was changed from 200% to 5% in the test series and a failure of a high pressure core spray (HPCS) diesel generator was assumed. Three power curves which represented conservative, realistic and zero initial stored heat, respectively, were used. In a large break LOCA such as 200% or 50% breaks, the initial stored heat in a fuel rod has a large effect on the cladding surface temperature because core uncovery occurs before all the initial stored heat is released, whereas in a small break LOCA such as a 5% break little effect is observed because core uncovery occurs after the initial stored heat is released. The maximum PCTs for the conservative initial stored heat case was 925 K, obtained in the 50% break experiment, and that for the realistic initial stored heat case was 835 K, obtained in the 5% break experiment. (orig./HP)

  16. Pecando en el deleite de la meditación: sobre la narrativa de Rosa Chacel

    Directory of Open Access Journals (Sweden)

    Ferrús Antón, Beatriz

    2006-06-01

    Full Text Available Rosa Chacel’s narrative participates in a same search: the way to the ONE; but also the bond’s between knowledge and misfortune search, like expression of a mystical individual. The objective of this article is to try to present this bet, that is the philosophy’s bet, but also the praxis of writing’s bet, through the analysis of two novels Memorias de Leticia Valle and La sinrazón. We’re going to put special attention to the relation between body, woman and memory like reading key.El conjunto de la narrativa de Rosa Chacel participa de una misma búsqueda: la del camino que lleva a lo UNO; pero también la del vínculo entre conocimiento y desdicha, como expresión de una particular mística. El objetivo de este artículo es tratar de presentar esta apuesta, que es la de una filosofía, pero también la de una praxis de escritura, a través del análisis de dos novelas Memorias de Leticia Valle y La sinrazón. Asimismo, se prestará especial atención a la relación entre cuerpo, mujer y memoria como clave de lectura.

  17. Reacciones Adversas al Tratamiento Antirretroviral de Gran Actividad Inicial en el Hospital Santa Rosa - Piura.

    Directory of Open Access Journals (Sweden)

    Jiovany Jhan Carlos Saldaña-Gastulo

    2009-01-01

    Full Text Available Objetivo, Determinar la incidencia y factores asociados a reacciones adversas medicamentosas (RAM al TARGA inicial durante el periodo enero 2006 - diciembre 2007 en el Hospital de Apoyo Santa Rosa-Piura. Diseño, Se realizó un estudio analítico de cohorte retrospectiva. Lugar, Hospital de Apoyo Santa Rosa-Piura. Participantes, 107 historias clínicas de pacientes VIH positivos mayores de 18 años que recibieron TARGA y fueron atendidos durante este periodo en el consultorio de infectología. Intervenciones, Se analizaron retrospectivamente las historias clínicas que fueron pertinentes para la recolección de datos. Principales medidas de resultado, Analisis Univariado, Bivariado (t de Student, y Riesgo Relativo, multivariado (Regresión logística con 95% de confianza (p<0.05. Resultados, En 107 pacientes se encontró una incidencia acumulada de 66,35% de RAM al TARGA inicial. Las RAM experimentadas con mayor frecuencia fueron anemia (35,2%, rash (16,9% y vómitos (9,9%. Se encontró diferencias significativas entre la media de edad y la presencia de RAM con la prueba de t de student (p < 0.05 Conclusiones, Existe una alta incidenica de RAM en pacientes incluidos en TARGA, siendo la más frecuente la anemia en 25 pacientes (35,2%. La media de edad es mayor en pacientes con RAM.

  18. Cytotoxic and genotoxic studies of essential oil from Rosa damascene Mill., Kashan, Iran.

    Science.gov (United States)

    Shokrzadeh, Mohammad; Habibi, Emran; Modanloo, Mona

    2017-08-01

    Aim Rosa damascene Mill. belongs to the family of Roseaceae and its essential oil is produced in large amounts in Iran. The wide application of rose oil has raised questions about potential adverse health effects. We have investigated cytotoxic activity and genotoxic effects of Rosa oil from Kashan, Iran. Methods The cytotoxic effect and IC50 of the essential oil on the cell lines was studied followed by MTT assay. In this assay mitochondrial oxidoreductase enzymes with reducing the tetrazolium dye MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) reflect the number of viable cells. Genotoxic effect of the oil was evaluated by micronucleus assay by evaluating produced micronuclei due to cytogenetic damage in binucleated lymphocytes. Results The results showed that essential oil significantly had cytotoxic and genotoxic effects at doses over 10µg/mL (pessential oil of Rose showed lower IC50 in cancer cell line (A549) in comparison with the normal cell line (NIH3T3). Conclusion Cytotoxic and genotoxic properties of essential oil of Rose in Kashan, Iran, are safe at a dose of 10µg/mL. Also, a good cytotoxic effect was shown and could be introduced as an anticancer compound. Further studies are needed with regard to anti-cancer effects of Rose essential oil. Copyright© by the Medical Assotiation of Zenica-Doboj Canton.

  19. Santa Rosa Geysers Recharge Project: GEO-98-001. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Brauner, Edwin Jr.; Carlson, Daniel C.

    2002-10-01

    The Geysers steamfields in northern Sonoma County have produced reliable ''green'' power for many years. An impediment to long-term continued production has been the ability to provide a reliable source of injection water to replace water extracted and lost in the form of steam. The steamfield operators have historcially used cooling towers to recycle a small portion of the steam and have collected water during the winter months using stream extraction. These two sources, however, could not by themselves sustain the steamfield in the long term. The Lake County Reclaimed Water Project (SEGEP) was inititated in 1997 and provides another source of steamfield replenishment water. The Santa Rosa Geysers Recharge Project provides another significant step in replenishing the steamfield. In addition, the Santa Rosa Geysers Recharge Project has been built with capacity to potentially meet virtually all injection water requirements, when combined with these other sources. Figure 2.1 graphically depicts the combination of injection sources.

  20. THE DEVELOPMENT OF TECHNOLOGY CUPCAKES USING ADDITIVES ROSEHIP MAY (ROSA MAJALIS

    Directory of Open Access Journals (Sweden)

    E. V. Belokurova

    2014-01-01

    Full Text Available Summary. This article contains information about newly developed technologies cupcakes with making fine powder (product recycling and water extract of the fruit of the wild rose may (Rosa majalis. Given the chemical composition of the deposited product of the processing of the fine powder hips may. The aim of this study is to develop and scientific substantiation of technologies of production of flour confectionery products, in particular cupcakes, with superior quality with the use of additives rosehip may. The results of experimental studies on changes of physico-chemical properties of the dough during fermentation, depending on the amounts of applied additives and modifying complex indicators of the quality of finished products. Based on the data produced optimal ratios make product recycling and extract from the fruits of medicinal plants in Central Russia hips may (Rosa majalis recipe cake products. See recipe cake products with optimal ratios introduced, enriching the product components. Based on the results of organoleptic and physico-chemical evaluation of the quality of finished products obtained from the samples of the test, the values obtained comprehensive quality indicators. The conclusion is that the best physico-chemical and organoleptic quality, has been studied in a laboratory study, are the samples with the introduction of the product refining of fine powder rosehip 3.6 % from the total amount of wheat flour and rosehip extract 5.7 % instead of the water in the recipe.

  1. Analgesic activity of Hibiscus rosa sinensis Linn in rat

    Directory of Open Access Journals (Sweden)

    Alka Sawarkar

    Full Text Available The plant Hibiscus rosa  sinensis Linn. belongs to family “Malvacecae” and Considering various medicinal properties of this plant, the leaves were collected and studied for Extractability percentage, analgesic. The herb Hibiscus rosa  sinensis  belonging to the family “Malvacecae” and is commonly known as jasvand. It is cultivated in garden throughout India native country probably china. The buds have a sweet odour and bitter taste, cooling, astringent, remove burning sensations of the body and relieve pain. In the present study aqueous, alcoholic Extracts of dried leaves of Hibiscus rosasinensis was prepared. The extractability percentage of leaves was 20%. The extract showed marked analgesic activity in a dose dependent manner. Aqueous and alcoholic extracts produced significant results at both doses (P<0.01, the findings indicated the analgesic activity of the leaves of the plant. [Vet World 2009; 2(9.000: 353-354

  2. Post-test analysis of ROSA-III experiment Run 702

    International Nuclear Information System (INIS)

    Koizumi, Yasuo; Kikuchi, Osamu; Soda, Kunihisa

    1980-01-01

    The purpose of the ROSA-III experiment with a scaled BWR test facility is to examine primary coolant thermal-hydraulic behavior and performance of ECCS during a posturated loss-of-coolant accident of BWR. The results provide information for verification and improvement of reactor safety analysis codes. Run 702 assumed a 200% split break at the recirculation pump suction line under an average core power without ECCS activation. Post - test analysis of the Run 702 experiment was made with computer code RELAP4J. Agreement of the calculated system pressure and the experiment one was good. However, the calculated heater surface temperatures were higher than the measured ones. Also, the axial temperature distribution was different in tendency from the experimental one. From these results, the necessity was indicated of improving the analytical model of void distribution in the core and the nodalization in the pressure vassel, in order to make the analysis more realistic. And also, the need of characteristic test was indicated for ROSA-III test facility components, such as jet pump and piping form loss coefficient; likewise, flow rate measurements must be increased and refined. (author)

  3. On the way to a self-reliance of 90 %. The new construction of the Monte Rosa Lodge in the Swiss Canton Wallis; Auf dem Weg zu 90% Autarkie. Der Neubau der Monte Rosa-Huette im Wallis

    Energy Technology Data Exchange (ETDEWEB)

    Sinn, Ruediger

    2010-07-01

    The new Monte Rosa Lodge in the Swiss Canton Wallis was established not only as a passive house, but also achieves a degree of self-reliance of 90 % due to a thought-out energy management. This is how the future of the alpine construction might look.

  4. Analisis Kinerja CV. Fabamus Famili Utama Semarang Dengan Balanced Scorecard

    OpenAIRE

    Permata, Nilla Mega; Nugraha, Hari Susanta; Nurseto, Sendhang

    2014-01-01

    A method of measuring the performance of the company to assist in knowing the health condition of the company itself. The study was conducted on the CV. Fabamus First Family with Balanced Scorecard approach to measuring through four perspectives: financial perspective, customer perspective, internal business process perspective and learning and growth perspectives. In the financial aspects of performance measurement with a weight of 70%, 10% customer perspective, internal business process per...

  5. STRATEGI PEMASARAN PRODUK DIGITAL PRINTING PADA CV. FNB DIGITAL JAMBI

    OpenAIRE

    Yuniarti, Yenni; Mauliana, Sarah

    2012-01-01

    CV. Digital FNB is one of the companies that involved in Digital Printing business. Based on survey in the middle of 2011-2012, there are 31 digital printing businesses in the city of Jambi. In order to examine the marketing stratey implemented, descriptive study has been conducted, uses interview, observation, and documentation as instruments. The strategy that observed in this study consist of marketing strategy including market segmentation, targeting, product strategy, pricing strategy, ...

  6. Applications in Foreign Currency Prima CV Ellindo Money Changer

    OpenAIRE

    Wisnu Sutrisno; Ire Puspa Wardhani, Skom, MMSI

    2004-01-01

    This explains the scientific writing about the design of application programs forforeign currency transactions by using Visual Basic 6.0 programming language.In scientific writing database is also used by Visual Data Manager (VisData)integrated in Visual Basic 6.0 program itself. Consists of three tables namelyCurrency,Customer and Transaction.In the end application program for foreign currency transactions will be applied to theCV. Prima Ellindo Money Changer for ease in performing activitie...

  7. The draft genome of Corchorus olitorius cv. JRO-524 (Navin

    Directory of Open Access Journals (Sweden)

    Debabrata Sarkar

    2017-06-01

    Full Text Available Here, we present the draft genome (377.3 Mbp of Corchorus olitorious cv. JRO-524 (Navin, which is a leading dark jute variety developed from a cross between African (cv. Sudan Green and indigenous (cv. JRO-632 types. We predicted from the draft genome a total of 57,087 protein-coding genes with annotated functions. We identified a large number of 1765 disease resistance-like and defense response genes in the jute genome. The annotated genes showed the highest sequence similarities with that of Theobroma cacao followed by Gossypium raimondii. Seven chromosome-scale genetically anchored pseudomolecules were constructed with a total size of 8.53 Mbp and used for synteny analyses with the cocoa and cotton genomes. Like other plant species, gypsy and copia retrotransposons were the most abundant classes of repeat elements in jute. The raw data of our study are available in SRA database of NCBI with accession number SRX1506532. The genome sequence has been deposited at DDBJ/EMBL/GenBank under the accession LLWS00000000, and the version described in this paper will be the first version (LLWS01000000.

  8. Hydrosols of orange blossom (Citrus aurantium), and rose flower (Rosa damascena and Rosa centifolia) support the growth of a heterogeneous spoilage microbiota.

    Science.gov (United States)

    Labadie, Cécile; Ginies, Christian; Guinebretiere, Marie-Hélène; Renard, Catherine M G C; Cerutti, Céline; Carlin, Frédéric

    2015-10-01

    Hydrosols are hydrodistillation products of aromatic plants. They contain less than 1g/L of dispersed essential oils giving organoleptic properties. Hydrosols are subjected to microbial proliferation. Reasons for spoilage have to be found in the nature of substrates supporting growth and of microbiological contaminants. The composition in essential oils and the microbiota of 22 hydrosol samples of Citrus aurantium L. ssp. amara L. (orange blossom), Rosa damascena Miller (rose D.), and Rosa centifolia L. (rose C.) flowers were analyzed to determine the factors responsible for decay. The median concentrations in essential oils were 677mg/L for orange blossom hydrosols, 205mg/L for rose D. hydrosols, and 116mg/L for rose C. hydrosols. The dry matter content of these hydrosols varied between 4.0mg/L and 702mg/L, and the carbohydrate content varied between 0.21mg/L and 0.38mg/L. These non-volatile compounds were likely carried over during distillation by a priming and foaming effect, and could be used as nutrients by microorganisms. A microbial proliferation at ambient temperature and also at 5°C has been observed in all studied hydrosols when stored in a non-sterile container. In contaminated hydrosols, maximal counts were about 7log 10 CFU/mL, while the French pharmacopeia recommends a maximal total bacterial count of 2log 10 CFU/mL. Neither yeast nor mold was detected. The isolated microbial population was composed of environmental Gram-negative bacteria, arranged in four major genera: Pseudomonas sp., Burkholderia cepacia complex, and presumably two new genera belonging to Acetobacteraceae and Rhodospirillaceae. Among those bacteria, Burkholderia vietnamiensis and Novosphingobium capsulatum were able to metabolize volatile compounds, such as geraniol to produce 6-methyl-5-hepten-2-one or geranic acid, or phenylethyl acetate to produce 2-phenylethanol. EO concentrations in hydrosols or cold storage are not sufficient to insure microbiological stability. Additional

  9. First formate, acetate and methanesulfonate analyses in firn samples from Grenzgletscher (Monte Rosa, 4200 m a.s.l.)

    Energy Technology Data Exchange (ETDEWEB)

    Grund, A.; Schwikowski, M.; Bruetsch, S.; Gaeggeler, H.W. [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    1997-09-01

    In order to determine trace concentrations of acetate, formate and methanesulfonate in ice samples by ion chromatography precautions have to be taken to avoid contaminations. We investigated sources of contamination and analysed first samples from Grenzgletscher (Monte Rosa massif). (author) 1 fig., 3 refs.

  10. The Role of Leaves in Photocontrol of Flower Bud Abscission in Hibiscus rosa-sinensis L. 'Nairobi'

    NARCIS (Netherlands)

    Meeteren, van U.; Gelder, van A.

    2000-01-01

    When compared with exposure to darkness, exposing Hibiscus rosa-sinensis L. 'Nairobi' plants to red light (635 to 685 nm, 2.9 μmol?m-2?s-1) delayed flower bud abscission, while exposure to far-red light (705 to 755 nm, μmol?m-2?s-1) accelerated this process. Flower bud abscission in response to

  11. Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa canina L.)

    DEFF Research Database (Denmark)

    Saaby, Lasse; Jäger, Anna Katharina; Moesby, Lise

    2011-01-01

    A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and...

  12. Population genetics of the potentially invasive African fruit fly species, Ceratitis rosa and Ceratitis fasciventris (Diptera: Tephritidae).

    Science.gov (United States)

    Baliraine, F N; Bonizzoni, M; Guglielmino, C R; Osir, E O; Lux, S A; Mulaa, F J; Gomulski, L M; Zheng, L; Quilici, S; Gasperi, G; Malacrida, A R

    2004-03-01

    A set of 10 microsatellite markers was used to survey the levels of genetic variability and to analyse the genetic aspects of the population dynamics of two potentially invasive pest fruit fly species, Ceratitis rosa and C. fasciventris, in Africa. The loci were derived from the closely related species, C. capitata. The degree of microsatellite polymorphism in C. rosa and C. fasciventris was extensive and comparable to that of C. capitata. In C. rosa, the evolution of microsatellite polymorphism in its distribution area reflects the colonization history of this species. The mainland populations are more polymorphic than the island populations. Low levels of differentiation were found within the Africa mainland area, while greater levels of differentiation affect the islands. Ceratitis fasciventris is a central-east African species. The microsatellite data over the Uganda/Kenya spatial scale suggest a recent expansion and possibly continuing gene flow within this area. The microsatellite variability data from C. rosa and C. fasciventris, together with those of C. capitata, support the hypothesis of an east African origin of the Ceratitis spp.

  13. The distribution, relative abundance, and seasonal phenology of Ceratitis capitata, Ceratitis rosa, and Ceratitis cosyra (Diptera: Tephritidae) in South Africa.

    Science.gov (United States)

    De Villiers, Marelize; Manrakhan, Aruna; Addison, Pia; Hattingh, Vaughan

    2013-10-01

    Ceratitis capitata (Wiedemann), Ceratitis rosa Karsch, and Ceratitis cosyra (Walker) are fruit fly species (Diptera: Tephritidae) of economic importance in South Africa. These pests cause direct damage to a number of commercially produced fruit and are of phytosanitary concern. A study was conducted to determine the distribution, relative abundance, and seasonal occurrence of the three species in different climatic regions of South Africa. The relative abundance and seasonal phenology of C. capitata and C. rosa were also compared between production areas and home gardens in Stellenbosch, Western Cape. Yellow bucket traps baited with Biolure were used to trap the flies over a 2-yr period in the different sampling areas. Different fruit types were sampled in Stellenbosch to determine fruit fly infestation. C. capitata was found to have a widespread distribution in South Africa, whereas C. rosa were absent from or only present in low numbers in the drier regions. C. cosyra was restricted to the North East and East coast, following a similar pattern to the distribution of marula, Sclerocarrya birrea, an important wild host. Fruit in home gardens provided a breeding ground for C. capitata and C. rosa and a source for infestation of orchards when fruit started to mature, highlighting the need for an area-wide strategy for the control of fruit flies.

  14. First formate, acetate and methanesulfonate analyses in firn samples from Grenzgletscher (Monte Rosa, 4200 m a.s.l.)

    International Nuclear Information System (INIS)

    Grund, A.; Schwikowski, M.; Bruetsch, S.; Gaeggeler, H.W.

    1997-01-01

    In order to determine trace concentrations of acetate, formate and methanesulfonate in ice samples by ion chromatography precautions have to be taken to avoid contaminations. We investigated sources of contamination and analysed first samples from Grenzgletscher (Monte Rosa massif). (author) 1 fig., 3 refs

  15. The Effect of Medium Cultures on Water Use and Charactristic of Gazania Flowers (Gazania hybrida in Green Roof.

    Directory of Open Access Journals (Sweden)

    Tahereh Bahrami

    2017-09-01

    Full Text Available Introduction: Green roof is one of the newest phenomenons in architecture and urbanism that refers to the sustainable development concepts and it will be usable for increasing landscape design, improving quality of the environment and reduction in energy consumption. Ensure of existing adequate green landscape in urban areas and improving access to natural areas surrounding the cities can help to offset negative effects of urban life. The use of green roof technology in cities is one of advanced techniques of green landscape. A green or living roof is a roof of a building that is partially or completely covered with vegetation and a growing medium on top view of buildings. Green roof layers that considered for roof side consist of protection layer, drainage layer, growing medium and plant layer. Medium layer is the medium culture of green roof that plants are begins to grow in it. This space should enable to save enough minerals and water for conserve of green-roof plants. All kinds of plants can growth on the green roof, but there are some constraints in creative of design because of roots dimension, plant canopy, necessary volume of soil, suitable direction to light, good weather, weight of designed structures, budget of repairing and keeping. Materials and Methods: To evaluate the effect of some culture medium on water consumption, vegetative and reproductive traits of Gazania (Gazania hybrida in condition of green roof a factorial experiment was conducted based on a completely randomized design with nine treatments and three replications in 2014. Treatments were three levels of vermicompost (zero, 5%, and 10% and rice hull (zero, 7, and 14%. Seedlings of plants cultivated in the media mixture of coco peat 15%, perlite 15%, leaf 10%, manure 10%, and filed soil 50%. The container had 60 × 60 ×25 cm dimensions that placed on the roof of greenhouse building with four meters height. The measured traits was number, average, and diameter of

  16. The Effect of Medium Cultures on Water Use and Charactristic of Gazania Flowers (Gazania hybrida in Green Roof.

    Directory of Open Access Journals (Sweden)

    Tahereh Bahrami

    2017-02-01

    Full Text Available Introduction: Green roof is one of the newest phenomenons in architecture and urbanism that refers to the sustainable development concepts and it will be usable for increasing landscape design, improving quality of the environment and reduction in energy consumption. Ensure of existing adequate green landscape in urban areas and improving access to natural areas surrounding the cities can help to offset negative effects of urban life. The use of green roof technology in cities is one of advanced techniques of green landscape. A green or living roof is a roof of a building that is partially or completely covered with vegetation and a growing medium on top view of buildings. Green roof layers that considered for roof side consist of protection layer, drainage layer, growing medium and plant layer. Medium layer is the medium culture of green roof that plants are begins to grow in it. This space should enable to save enough minerals and water for conserve of green-roof plants. All kinds of plants can growth on the green roof, but there are some constraints in creative of design because of roots dimension, plant canopy, necessary volume of soil, suitable direction to light, good weather, weight of designed structures, budget of repairing and keeping. Materials and Methods: To evaluate the effect of some culture medium on water consumption, vegetative and reproductive traits of Gazania (Gazania hybrida in condition of green roof a factorial experiment was conducted based on a completely randomized design with nine treatments and three replications in 2014. Treatments were three levels of vermicompost (zero, 5%, and 10% and rice hull (zero, 7, and 14%. Seedlings of plants cultivated in the media mixture of coco peat 15%, perlite 15%, leaf 10%, manure 10%, and filed soil 50%. The container had 60 × 60 ×25 cm dimensions that placed on the roof of greenhouse building with four meters height. The measured traits was number, average, and diameter of

  17. Evaluation of the wound-healing activity of Hibiscus rosa sinensis L (Malvaceae) in Wistar albino rats

    Science.gov (United States)

    Bhaskar, Anusha; Nithya, V.

    2012-01-01

    Objective: To investigate the wound-healing potency of the ethanolic extract of the flowers of Hibiscus rosa sinensis. Materials and Methods: The wound-healing activity of H. rosa sinensis (5 and 10% w/w) on Wistar albino rats was studied using three different models viz., excision, incision and dead space wound. The parameters studied were breaking strength in incision model, granulation tissue dry weight, breaking strength and collagen content in dead space wound model, percentage of wound contraction and period of epithelization in excision wound model. The granulation tissue formed on days 4, 8, 12, and 16 (post-wound) was used to estimate total collagen, hexosamine, protein, DNA and uronic acid. Data were analyzed by Analysis of Variance (ANOVA) test. P<0.05 was considered statistically significant. Results: The extract increased cellular proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and total collagen content of granulation tissues. The extract-treated wounds were found to heal much faster as indicated by improved rates of epithelialization and wound contraction. The extract of H. rosa sinensis significantly (P<0.001) increased the wound-breaking strength in the incision wound model compared to controls. The extract-treated wounds were found to epithelialize faster, and the rate of wound contraction was significantly (P<0.001) increased as compared to control wounds. Wet and dry granulation tissue weights in a dead space wound model increased significantly (P<0.001). There was a significant increase in wound closure rate, tensile strength, dry granuloma weight, wet granuloma weight and decrease in epithelization period in H. rosa sinensis-treated group as compared to control and standard drug-treated groups. Conclusion: The ethanolic extract of H. rosa sinensis had greater wound-healing activity than the nitrofurazone ointment. PMID:23248396

  18. SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida

    Directory of Open Access Journals (Sweden)

    Yongbiao eXue

    2014-07-01

    Full Text Available Many flowering plants adopt self-incompatibility (SI to maintain their genetic diversity. In species of Solanaceae, Plantaginaceae and Rosaceae, SI is genetically controlled by a single S-locus with multiple haplotypes. The S-locus has been shown to encode S-RNases expressed in pistil and multiple SLF (S-locus F-box proteins in pollen controlling the female and male specificity of SI, respectively. S-RNases appear to function as a cytotoxin to reject self-pollen. In addition, SLFs have been shown to form SCF (SKP1/Cullin1/F-box complexes to serve as putative E3 ubiquitin ligase to interact with S-RNases. Previously, two different mechanisms, the S-RNase degradation and the S-RNase compartmentalization, have been proposed as the restriction mechanisms of S-RNase cytotoxicity allowing compatible pollination. In this study, we have provided several lines of evidence in support of the S-RNase degradation mechanism by a combination of cellular, biochemical and molecular biology approaches. First, both immunogold labeling and subcellular fractionation assays showed that two key pollen SI factors, PhSLF-S3L and PhSSK1 (SLF-interacting SKP1-like1 from Petunia hybrida, a Solanaceous species, are co-localized in cytosols of both pollen grains and tubes. Second, PhS3L-RNases are mainly detected in the cytosols of both self and non-self pollen tubes after pollination. Third, we found that both PhS3-RNases and PhS3L-RNases directly interact with PhSLF-S3L by yeast two-hybrid and co-immunoprecipitation assays. Fourth, S-RNases are specifically degraded in compatible pollen tubes by non-self SLF action. Taken together, our results demonstrate that SCFSLF-mediated non-self S-RNase degradation occurs in the cytosol of pollen tube through the ubiquitin/26S proteasome system serving as the major mechanism to neutralize S-RNase cytotoxicity during compatible pollination in P. hybrida.

  19. Analisis Daya Saing untuk Meningkatkan Kualitas Layanan terhadap Pelanggan dengan Pendekatan CRM di CV. BLITZSPOT

    Directory of Open Access Journals (Sweden)

    Suryadi Muhammad

    2017-04-01

    Full Text Available CV. Blitzspot adalah perusahaan yang bergerak di bidang jasa penyediaan layanan internet (internet service provider, yang berkantor pusat di Komplek Padasuka Indah Ruko Blok A-1, Cimahi, Jawa Barat. Berdasarkan hasil wawancara dan hasil observasi pada data transaksi CV. Blitzspot, konsumen CV. Blitzspot yang melakukan transaksi tiap tahunnya selalu berkurang, hal ini dikarenakan konsumen sering membandingkan layanan-layanan yang ditawarkan oleh para pesaing CV. Blitzspot. Bila permasalahan tersebut belum teratasi, konsumen CV. Blitzspot akan menurun tiap tahunnya dan akan selalu berpindah ke pesaing CV. Blitzspot. Direktur Utama CV. Blitzspot ingin menganalisis daya saing untuk menentukan layanan-layanan yang akan diberikan kepada pelanggan CV. Blitzspot dan penentuan strategi untuk mempertahankan konsumen CV. Blitzspot.  Metode Analisis daya saing yang digunakan adalah Model Analisis Berlian Porter dan Metode Pengelompokkan Pelanggan yang digunakan adalah Metode LRFM. Berdasarkan hasil pengujian dan pembahasan yang telah dibuat, maka dapat ditarik kesimpulan bahwa Direktur Utama dapat menganalisis daya saing CV. Blitzspot dengan menggunakan Berlian Porter untuk meningkatkan kualitas layanan terhadap pelanggan CV. Blitzspot.

  20. Entrevista com Mathieu Dosse, Gian Luigi de Rosa e Michael Kegler

    Directory of Open Access Journals (Sweden)

    Andréia Guerini

    2017-09-01

    Full Text Available O conjunto das três entrevistas que seguem aborda a mesma temática, isto é, aspectos de tradução/adaptação em geral e da tradução do romance Estive em Lisboa e lembrei de você de Luiz Ruffato em particular. O romance de Ruffato foi escrito em 2009, editado em Portugal (Quetzal, 2010 e traduzido para diferentes línguas, como italiano (La Nuova Frontiera, 2011, espanhol (Eterna Cadencia, 2011, francês (Chandeigne, 2015, alemão (Assoziation A, 2016 e, nos próximos meses será publicado em finlandês (Into. O livro foi adaptado para o cinema em 2015, pelo cineasta português José Barahona, sendo exibido em festivais nacionais e internacionais. As entrevistas abaixo foram feitas com os tradutores Gian Luigi De Rosa, Mathieu Dosse e Michael Kegler em 2016. Gian Luigi De Rosa (Itália/1969-- possui doutorado em “Culture e Istituzioni dei paesi di lingue iberiche in età moderna e contemporanea” e é professor de português na Universidade do Salento, em Lecce. É autor de livros e ensaios sobre língua e linguística portuguesa, literatura portuguesa e brasileira e tradução audiovisual e intersemiótica. É também responsável pela elaboração das legendas em italiano do filme Estive em Lisboa e lembrei de você. Mathieu Dosse (Brasil/1978-- é formado pela Université Paris 8, onde estudou Teoria da Tradução. Traduziu para o francês Graciliano Ramos, Luiz Ruffato e João Guimarães Rosa. Michael Kegler (Alemanha/1967-- estudou literatura brasileira e portuguesa na universidade de Frankfurt, sem concluir o curso. Trabalhou como livreiro no Centro do Livro de Língua Portuguesa, antes de se tornar tradutor literário. Traduziu para o alemão José Eduardo Agualusa, Moacyr Scliar, Luiz Ruffato, entre outros autores. Em julho de 2016, junto com Ruffato, recebeu o prêmio literário Hermann Hesse na Alemanha, pela qualidade da obra publicada em conjunto com a sua tradução.

  1. Multiplicação in vitro da ameixeira 'Santa Rosa': efeito da citocinina BAP

    Directory of Open Access Journals (Sweden)

    Rogalski Marcelo

    2003-01-01

    Full Text Available A ameixeira 'Santa Rosa' apresenta alta produtividade, ótimo sabor e aparência dos frutos para a comercialização. No entanto, por ser altamente suscetível à escaldadura das folhas (Xylella fastidiosa Wells, esta variedade apresenta problemas de cultivo no sul do Brasil. As técnicas de cultura in vitro permitem propagar e rapidamente espécies de interesse, além de permitir a limpeza de patógenos e a produção de matrizes com qualidade genética e sanitária comprovada. Porém, o uso prático da propagação in vitro requer a otimização das condições de cultura para cada espécie e/ou variedade. Dentre os fatores que mais influenciam a micropropagação, estão as citocininas, com destaque para o BAP. O objetivo principal deste trabalho foi avaliar o potencial de multiplicação in vitro da ameixeira 'Santa Rosa' sob diferentes concentrações de BAP. Após três subculturas em meio MA1, segmentos nodais com 0,5-1,0 cm foram submetidos a diferentes concentrações de BAP (0,5; 1,0; 2,0 e 3,0 mg.L-1. Os resultados mostraram que não ocorreram diferenças significativas no número de brotos para as diferentes concentrações de BAP testadas. No entanto, o maior número de brotos por explante (3,6 obteve-se na concentração de 2,0 mg.L-1 e a maior altura média dos brotos foi obtido na concentração de 0,5 mg.L-1 de BAP. Concentrações maiores que a 0,5 mg.L-1 de BAP inibiram o crescimento dos brotos. A micropropagação da ameixeira 'Santa Rosa' a partir de ápices caulinares e gemas laterais em meio de cultura MA1 mostrou-se eficaz.

  2. Induction of single flower mutant in Hibiscus cv. 'Alipur Beauty'

    International Nuclear Information System (INIS)

    Banerji, B.K.; Datta, S.K.

    1986-01-01

    Stem cuttings of Hibicus cv. 'Alipur Beauty' were irradiated with 0, 1.0, 2.0 Krad of gamma rays in 60 Co source and planted just after irradiation. Data were recorded on days to sprout, number of sprouts, sprouting percentage, survival, plant height, leaf number and size, abnormal leaves and plants, floral abnormalities and induction of somatic mutations in flower colour/form. Some of the characters like plant height, leaf number, abnormal leaves and plants and leaf size were recorded after 60 and 120 days. 7 refs. (author)

  3. DETECTION OF MOTORCYCLISTS WITHOUT HELMET AND FINEPAYMENT USING OPEN CV

    OpenAIRE

    Stemy Simon, Divya Kumaran A.K.

    2018-01-01

    The helmet is the main safety equipment of motorcyclists, but many drivers do not use it. The main aim of this project is to construct an automatic detection of the motorcyclist without helmet from video using OpenCV library tools. If they are not wearing the helmet, the license plate of the motorcycle is focused automatically. By using Computer Vision technique we can detect and recognize the license plate number. We make the training set of different characters of different sizes. Based on ...

  4. Comparison of tracking algorithms implemented in OpenCV

    Directory of Open Access Journals (Sweden)

    Janku Peter

    2016-01-01

    Full Text Available Computer vision is very progressive and modern part of computer science. From scientific point of view, theoretical aspects of computer vision algorithms prevail in many papers and publications. The underlying theory is really important, but on the other hand, the final implementation of an algorithm significantly affects its performance and robustness. For this reason, this paper tries to compare real implementation of tracking algorithms (one part of computer vision problem, which can be found in the very popular library OpenCV. Moreover, the possibilities of optimizations are discussed.

  5. OpenCV 3.0 computer vision with Java

    CERN Document Server

    Baggio, Daniel Lélis

    2015-01-01

    If you are a Java developer, student, researcher, or hobbyist wanting to create computer vision applications in Java then this book is for you. If you are an experienced C/C++ developer who is used to working with OpenCV, you will also find this book very useful for migrating your applications to Java. All you need is basic knowledge of Java, with no prior understanding of computer vision required, as this book will give you clear explanations and examples of the basics.

  6. Real-time graphic display system for ROSA-V Large Scale Test Facility

    International Nuclear Information System (INIS)

    Kondo, Masaya; Anoda, Yoshinari; Osaki, Hideki; Kukita, Yutaka; Takigawa, Yoshio.

    1993-11-01

    A real-time graphic display system was developed for the ROSA-V Large Scale Test Facility (LSTF) experiments simulating accident management measures for prevention of severe core damage in pressurized water reactors (PWRs). The system works on an IBM workstation (Power Station RS/6000 model 560) and accommodates 512 channels out of about 2500 total measurements in the LSTF. It has three major functions: (a) displaying the coolant inventory distribution in the facility primary and secondary systems; (b) displaying the measured quantities at desired locations in the facility; and (c) displaying the time histories of measured quantities. The coolant inventory distribution is derived from differential pressure measurements along vertical sections and gamma-ray densitometer measurements for horizontal legs. The color display indicates liquid subcooling calculated from pressure and temperature at individual locations. (author)

  7. Investigation of Polyhenolic Content of Rose Hip (Rosa canina L. Tea Extracts: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Zeynep İlbay

    2013-02-01

    Full Text Available Three different brands of Rose hip (Rosa canina L. tea were extracted with water, ethanol (EtOH, methanol (MeOH, and aqueous mixtures (50%, v/v by ultrasound-assisted extraction (UAE and Soxhlet methods. Total phenolic content was determined according to the Folin-Ciocalteu method. The results were presented by means of the extract yields and total phenolic contents, expressed in gallic acid equivalent (GAE per g of dried matter (DM. The greatest amount of extract observed in tea samples was obtained by UAE through water with the value of 619.37 ± 0.58 mg/g DM. Regarding the phenolic content, the best result was achieved by the Soxhlet method through 50% MeOH mixture (59.69 ± 0.89 mg GAE/g DM, followed by the UAE method with water (48.59 ± 0.29 mg GAE/g DM.

  8. The Rosa genome provides new insights into the domestication of modern roses.

    Science.gov (United States)

    Raymond, Olivier; Gouzy, Jérôme; Just, Jérémy; Badouin, Hélène; Verdenaud, Marion; Lemainque, Arnaud; Vergne, Philippe; Moja, Sandrine; Choisne, Nathalie; Pont, Caroline; Carrère, Sébastien; Caissard, Jean-Claude; Couloux, Arnaud; Cottret, Ludovic; Aury, Jean-Marc; Szécsi, Judit; Latrasse, David; Madoui, Mohammed-Amin; François, Léa; Fu, Xiaopeng; Yang, Shu-Hua; Dubois, Annick; Piola, Florence; Larrieu, Antoine; Perez, Magali; Labadie, Karine; Perrier, Lauriane; Govetto, Benjamin; Labrousse, Yoan; Villand, Priscilla; Bardoux, Claudia; Boltz, Véronique; Lopez-Roques, Céline; Heitzler, Pascal; Vernoux, Teva; Vandenbussche, Michiel; Quesneville, Hadi; Boualem, Adnane; Bendahmane, Abdelhafid; Liu, Chang; Le Bris, Manuel; Salse, Jérôme; Baudino, Sylvie; Benhamed, Moussa; Wincker, Patrick; Bendahmane, Mohammed

    2018-06-01

    Roses have high cultural and economic importance as ornamental plants and in the perfume industry. We report the rose whole-genome sequencing and assembly and resequencing of major genotypes that contributed to rose domestication. We generated a homozygous genotype from a heterozygous diploid modern rose progenitor, Rosa chinensis 'Old Blush'. Using single-molecule real-time sequencing and a meta-assembly approach, we obtained one of the most comprehensive plant genomes to date. Diversity analyses highlighted the mosaic origin of 'La France', one of the first hybrids combining the growth vigor of European species and the recurrent blooming of Chinese species. Genomic segments of Chinese ancestry identified new candidate genes for recurrent blooming. Reconstructing regulatory and secondary metabolism pathways allowed us to propose a model of interconnected regulation of scent and flower color. This genome provides a foundation for understanding the mechanisms governing rose traits and should accelerate improvement in roses, Rosaceae and ornamentals.

  9. Claiming Rosa Parks: conservative Catholic bids for 'rights' in contemporary Latin America.

    Science.gov (United States)

    Morgan, Lynn M

    2014-01-01

    When the Rosa Parks Prize was awarded to a conservative Argentine senator in 2009 for her outspoken opposition to contraception, sterilisation and abortion, it was clear that something odd was happening. This paper documents the appropriation of 'human rights' discourses by conservative Catholics in Latin America, where the recent success of reproductive and sexual rights social movements has generated a significant backlash. It specifically traces an effort by Catholic legal scholars to justify what they term 'a distinctively Latin American approach to human rights' while ignoring decades of human rights activism by others. Opponents of reproductive and sexual rights are deploying rights-talk selectively and strategically, it is argued, using this as secular cover to advance pro-life and pro-family policies.

  10. Experiment on performance of upper head injection system with ROSA-II

    International Nuclear Information System (INIS)

    1976-09-01

    Thermo-hydraulic behavior in the primary cooling system of a pressurized water reactor with an upper head injection system (UHI) in a postulated loss-of-coolant accident (LOCA) has been studied with ROSA-II test facility. Simulated UHI and internal structures of the pressure vessel were installed to the facility for the experiment. Nine maximum-sized double-ended break tests and one medium-sized split break test were performed for the cold-leg break condition. The results are as follows: (1) Fluid mixing in the upper head is not perfect. (2) Cold water injection into the steam or two-phase fluid causes violent depressurization due to the condensation. Flow pattern in the primary cooling system is largely influenced by the above two. (auth.)

  11. Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts.

    Science.gov (United States)

    Nayak, Debasis; Ashe, Sarbani; Rauta, Pradipta Ranjan; Nayak, Bismita

    2015-10-01

    Green synthesis of metallic nanoparticles has lured the world from the chemical and physical approaches owing to its rapid, non-hazardous and economic aspect of production mechanism. In this study, silver nanoparticles (AgNPs) were synthesised using petal extracts of Hibiscus rosa-sinensis. The AgNPs displayed characteristic surface plasmon resonance peak at around 421 nm having a mean particle size of 76.25±0.17 nm and carried a charge of -41±0.2 mV. The X-ray diffraction patterns displayed typical peaks of face centred cubic crystalline silver. The surface morphology was characterised by scanning electron microscopy and atomic force microscopy. Fourier transform infrared spectroscopy studies confirmed the surface modifications of the functional groups for the synthesis of AgNPs. Furthermore, the synthesised AgNPs displayed proficient antimicrobial activity against pathogenic strains of Vibrio cholerae, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus.

  12. Methodology for the design of Santa Rosa de Cabal sanitary landfill, Risaralda

    International Nuclear Information System (INIS)

    Tabares, J; Orozco, J

    1992-01-01

    In 1987 the Regional Autonomous Corporation of Risaralda, CARDER and the Risaralda Government, they signed a cooperation agreement, in order to endowing from sanitary landfill to the municipalities of the department. In the mark of this agreement it was carried out the design of Santa Rosa's sanitary landfill, that with near 50.000 inhabitants it is constituted in the third city of the department. This city generates some 25 tons/day of garbage that at the present time are heady directly to the waters of San Eugenio River. The present work contains the most important methodological aspects in the design of the sanitary landfill and some comments about the approaches ideal Vs real approaches of selection of places

  13. Investigation of Polyhenolic Content of Rose Hip (Rosa canina L.) Tea Extracts: A Comparative Study

    Science.gov (United States)

    İlbay, Zeynep; Şahin, Selin; Kırbaşlar, Ş. İsmail

    2013-01-01

    Three different brands of Rose hip (Rosa canina L.) tea were extracted with water, ethanol (EtOH), methanol (MeOH), and aqueous mixtures (50%, v/v) by ultrasound-assisted extraction (UAE) and Soxhlet methods. Total phenolic content was determined according to the Folin-Ciocalteu method. The results were presented by means of the extract yields and total phenolic contents, expressed in gallic acid equivalent (GAE) per g of dried matter (DM). The greatest amount of extract observed in tea samples was obtained by UAE through water with the value of 619.37 ± 0.58 mg/g DM. Regarding the phenolic content, the best result was achieved by the Soxhlet method through 50% MeOH mixture (59.69 ± 0.89 mg GAE/g DM), followed by the UAE method with water (48.59 ± 0.29 mg GAE/g DM). PMID:28239095

  14. Gel state of the fictional body in Mario Bellatin’s 'Rosa Canina' and "Flores"

    Directory of Open Access Journals (Sweden)

    Isabel Jasinski

    2015-12-01

    Full Text Available Mario Bellatin creates a peculiar "space of sense" in fiction, related to the visual and per­formatic expressions of language. So, his literary project presents writing as body language, a terri­tory of significance constantly deterritorialized by sensations connected to the narrative game, with images and symbols in the literary actions. A sense of creative destruction constitutes the paradox expressed by fiction as body and by the body as fiction, in what we call Bellatin’s "nomadic writing", by means of the abnormality of the characters, their sexuality and religiosity, as one can note in Flo­res (2000, especially in "Rosa canina", published online by Cosac Naify in 2009. The position adopted by the protagonist in this work seems to indicate a specific "regime of signification”, as Josefina Ludmer puts it, according to which art and reality, fiction and biography blur their borders in a “gel state exchange”.

  15. Supplemental description of ROSA-IV/LSTF with No.1 simulated fuel-rod assembly

    International Nuclear Information System (INIS)

    1989-09-01

    Forty-two integral simulation tests of PWR small break LOCA (loss-of-coolant accident) and transient were conducted at the ROSA-IV Large-Scale Test Facility (LSTF) with the No.1 simulated fuel-rod assembly between March 1985 and August 1988. Described in the report are supplemental information on modifications of the system hardware and measuring systems, results of system characteristics tests including the initial fluid mass inventory and heat loss distribution for the primary system, and thermal properties for the heater rod materials. These are necessary to establish the correct boundary conditions of each LSTF experiment with the No.1 core assembly in addition to the system data given in the system description report (JAERI-M 84-237). (author)

  16. Yağ Gülü (Rosa damascena Mill'nde Mutasyon Islahı

    Directory of Open Access Journals (Sweden)

    Hasan BAYDAR

    2014-07-01

    Full Text Available Mutation breeding is one of the ways to create genetic variation of oil rose, as in other species. In the present study, it is aimed to develop superior mutants in terms of mophological, agronomic and quality characteristic compare to the oil rose. Oil rose fruits (Rosa damascena Mill. were harvested and seeds were extracted in 2007. 1000 seeds were irradiated with 0, 100, 200, 300, 400 Gry doses of the cobalt 60 gamma-ray in Atomic Energy Agency, Ankara, Turkey. Irradiated seeds were stratified at 4oC for 3 month and planted to viols on March in 2008. Seedlings applied 0, 100, 200 Gry doses were transferred to pots in 2008 and were planted to field on March in 2010. Mutant oil roses showed wide genetic variation for flower color (from darker pink to white, petal number (from 5 to 90 and flowering period (M1 generation in 2011

  17. ROSA-IV Large Scale Test Facility (LSTF) system description for second simulated fuel assembly

    International Nuclear Information System (INIS)

    1990-10-01

    The ROSA-IV Program's Large Scale Test Facility (LSTF) is a test facility for integral simulation of thermal-hydraulic response of a pressurized water reactor (PWR) during small break loss-of-coolant accidents (LOCAs) and transients. In this facility, the PWR core nuclear fuel rods are simulated using electric heater rods. The simulated fuel assembly which was installed during the facility construction was replaced with a new one in 1988. The first test with this second simulated fuel assembly was conducted in December 1988. This report describes the facility configuration and characteristics as of this date (December 1988) including the new simulated fuel assembly design and the facility changes which were made during the testing with the first assembly as well as during the renewal of the simulated fuel assembly. (author)

  18. Impact of invasive Rosa rugosa on the arthropod fauna of Danish yellow dunes

    DEFF Research Database (Denmark)

    Elleriis, Pernille; Pedersen, Morten Lauge; Toft, Søren

    2015-01-01

    monospecific shrubbery rich in large flowers. We predicted faunal responses according to the changes in resource availability and environmental conditions promoted by this particular invasive plant: increased populations of flower-visiting insects and species of the phytophagous and detritivorous guilds...... and diversity and increased dominance in the rose patches, due to reductions among xerotherm species. The results indicate that considerable faunistic impoverishment of thermophilic dune specialist species can be expected in the future if R. rugosa is allowed to continue its invasion across the dune habitat.......We compared the arthropod fauna of Rosa rugosa patches to the adjacent native yellow dune vegetation by pitfall trapping in the National Park Thy at the Danish North Sea coast. R. rugosa changes the vegetation from a dune grassland (dominated by Ammophila arenaria) poor in flowering plants to a low...

  19. Effects of Harvest Times on the Fatty Acids Composition of Rose Hip (Rosa sp. Seeds

    Directory of Open Access Journals (Sweden)

    Mehmet Güneş

    2017-04-01

    Full Text Available This study was conducted to determine the change of fatty acids ratios of some rose hip species seeds in different harvest times. Seeds of five genotypes belonging to rose hip species (Rosa sp. were used in the study. The fruits of species were harvested in six different ripening times and analyzed. Total oil analysis was performed for the fifth harvest only, which was determined as the optimal harvest time. As a result; total oil ratio of rose hip seeds varied as 5.22 and 6.62 g/100g respectively for accessions of Rosa dumalis (MR-12 and MR-15, 6.37 g/100g for R. canina (MR-26, 5.00 g/100g for R. dumalis ssp. boissieri (MR-46 and 5.29 g/100g for R. villosa (MR-84. Eleven fatty acids were determined in rose hip seeds. Among these fatty acids linoleic, oleic, linolenic, palmitic and stearic acids respectively had high ratio. Saturated fatty acids ratio (SFAs was the highest in R. canina (MR-26 and the lowest in R. dumalis (MR-12; monounsaturated fatty acids ratio (MUFAs was the highest in R. dumalis (MR-12 and the lowest in R. dumalis ssp. boissieri (MR-46; polyunsaturated fatty acids ratio (PUFAs was the highest in R. dumalis ssp. boissieri (MR-46 and the lowest in R. dumalis (MR-12. Mono and polyunsaturated fatty acid contents obtained in this study was high; the change of fatty acid profile in the studied species in relation to harvest time was significant for some species and insignificant for others. A conclusion was reached that it is important to pay attention to qualitative and quantitative properties of seeds when conducting studies about rose hip improvement.

  20. Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations.

    Science.gov (United States)

    Wenzig, E M; Widowitz, U; Kunert, O; Chrubasik, S; Bucar, F; Knauder, E; Bauer, R

    2008-10-01

    The aim of the present study was to compare powdered rose hip with and without fruits (Rosae pseudofructus cum/sine fructibus, Rosa canina L., Rosaceae) with regard to their phytochemical profile and their in vitro anti-inflammatory and radical-scavenging properties. The two powders were subsequently extracted with solvents of increasing polarity and tested for inhibition of cyclooxygenase (COX-1, COX-2) and of 5-LOX-mediated leukotriene B(4) (LTB(4)) formation as well as for DPPH-radical-scavenging capacity. While the water and methanol extracts were inactive in the COX-1, COX-2 and LTB(4) inhibition assays, the n-hexane and the dichloromethane extracts inhibited all three enzymes. In the active extracts, the triterpenoic acids ursolic acid, oleanolic acid and betulinic acid were identified, although only in minute amounts. Furthermore, oleic, linoleic and alpha-linolenic acid were identified apart from several saturated fatty acids. Even though unsaturated fatty acids are known to be good inhibitors of COX-1, COX-2 and LT formation, no clear correlation between their concentration in the extracts and their activity was found. We suggest that other, yet unidentified, lipophilic constituents might play a more important role for the observed in vitro inhibitory activity on arachidonic acid metabolism. Some of the extracts also showed considerable DPPH radical scavenging activity, the methanolic extracts being most potent. The radical scavenging activity of the extracts correlated very well with their total phenolic content, while ascorbic acid contributes only little to the radical-scavenging activity due to its low concentration present in the extracts. In summary, extracts derived from powdered rose hip without fruits were more effective in all assays carried out compared with extracts derived from powdered rose hip with fruits.

  1. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

    Directory of Open Access Journals (Sweden)

    M. Kaleemullah

    2017-07-01

    Full Text Available Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor (f2 value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05 between the F3 and reference drug in terms of MDT and

  2. Reacciones adversas al tratamiento antirretroviral de gran actividad inicial en el hospital Santa Rosa – Piura.

    Directory of Open Access Journals (Sweden)

    Jiovany Jahn Carlos Saldaña-Gastulo

    2011-01-01

    Full Text Available Objetivo: Determinar la incidencia y factores asociados a reacciones adversas medicamentosas (RAM al TARGA inicial durante el periodo enero 2006 – diciembre 2007 en el Hospital de Apoyo Santa Rosa-Piura. Diseño: Se realizó un estudio analítico de cohorte retrospectiva. Lugar: Hospital de Apoyo Santa Rosa-Piura. Participantes: 107 historias clínicas de pacientes VIH positivos mayores de 18 años que recibieron TARGA y fueron atendidos durante este periodo en el consultorio de infectología. Intervenciones: Se analizaron retrospectivamente las historias clínicas que fueron pertinentes para la recolección de datos. Principales medidas de resultado: Analisis Univariado, Bivariado (t de Student, y Riesgo Relativo, multivariado (Regresión logística con 95% de confianza (p<0.05. Resultados: En 107 pacientes se encontró una incidencia acumulada de 66,35% de RAM al TARGA inicial. Las RAM experimentadas con mayor frecuencia fueron anemia (35,2%, rash (16,9% y vómitos (9,9%. Se encontró diferencias significativas entre la media de edad y la presencia de RAM con la prueba de t de student (p < 0.05 Conclusiones: Existe una alta incidenica de RAM en pacientes incluidos en TARGA, siendo la más frecuente la anemia en 25 pacientes (35,2%. La media de edad es mayor en pacientes con RAM. Palabras Claves: VIH/SIDA, Terapia de alta actividad antirretroviral, Reacciones adversas a medicamentos.

  3. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage.

    Science.gov (United States)

    Kaleemullah, M; Jiyauddin, K; Thiban, E; Rasha, S; Al-Dhalli, S; Budiasih, S; Gamal, O E; Fadli, A; Eddy, Y

    2017-07-01

    Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor ( f 2 ) value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f 2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05) between the F3 and reference drug in terms of MDT and T50% with p

  4. Association between mapped vegetation and Quaternary geology on Santa Rosa Island, California

    Science.gov (United States)

    Cronkite-Ratcliff, C.; Corbett, S.; Schmidt, K. M.

    2017-12-01

    Vegetation and surficial geology are closely connected through the interface generally referred to as the critical zone. Not only do they influence each other, but they also provide clues into the effects of climate, topography, and hydrology on the earth's surface. This presentation describes quantitative analyses of the association between the recently compiled, independently generated vegetation and geologic map units on Santa Rosa Island, part of the Channel Islands National Park in Southern California. Santa Rosa Island was heavily grazed by sheep and cattle ranching for over one hundred years prior to its acquisition by the National Park Service. During this period, the island experienced significant erosion and spatial reduction and diversity of native plant species. Understanding the relationship between geology and vegetation is necessary for monitoring the recovery of native plant species, enhancing the viability of restoration sites, and understanding hydrologic conditions favorable for plant growth. Differences in grain size distribution and soil depth between geologic units support different plant communities through their influence on soil moisture, while differences in unit age reflect different degrees of pedogenic maturity. We find that unsupervised machine learning methods provide more informative insight into vegetation-geology associations than traditional measures such as Cramer's V and Goodman and Kruskal's lambda. Correspondence analysis shows that unique vegetation-geology patterns associated with beach/dune, grassland, hillslope/colluvial, and fluvial/wetland environments can be discerned from the data. By combining geology and vegetation with topographic variables, mixture models can be used to partition the landscape into multiple representative types, which then be compared with conceptual models of plant growth and succession over different landforms. Using this collection of methods, we show various ways that that Quaternary geology

  5. Subcooled decompression analysis of the ROSA and the LOFT semiscale blowdown test data with the digital computer code DEPCO-MULTI

    International Nuclear Information System (INIS)

    Namatame, Ken; Kobayashi, Kensuke

    1975-12-01

    In the ROSA (Rig of Safety Assessment) program, the digital computer code DEPCO-SINGLE and DEPCO-MULTI (Subcooled Decompression Process in Loss-of-Coolant Accident - Single Pipe and - Multiple Pipe Network) were prepared to study thermo-hydraulic behavior of the primary coolant in subcooled decompression of the PWR LOCA. The analytical results with DEPCO-MULTI on the subcooled decompression phenomena are presented for ROSA-I, ROSA-II and LOFT 500, 600, 700 and 800 series experiments. The effects of space mesh length, elasticity of pressure boundary materials and simplification for computational piping system on the computed result are described. This will be the final work on the study of the subcooled decompression analysis as for the ROSA program, and the authors wish that the present code shall further be examined with the data of much advanced experiments. (auth.)

  6. Effects of Biopesticides on Foliar Diseases and Japanese Beetle (Popillia japonica) Adults in Roses (Rosa spp.), Oakleaf Hydrangea (Hydrangea quercifolia), and Crapemyrtle (Lagerstroemia indica)

    Science.gov (United States)

    This study evaluated efficacy of biopesticides for reducing foliar diseases and feeding damage from Japanese beetle adults on hybrid T rose (Rosa spp.), oakleaf hydrangea (Hydrangea quercifolia), and crapemyrtle (Lagerstroemia indica). The materials tested included household soaps with Triclosan act...

  7. Sterile mutant of Verbena hybrida induced by heavy ion beam irradiation and wild species V. peruviana exhibit self-incompatible phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Saito, H; Hayashi, Y; Abe, T [RIKEN, Wako (Japan); Kanaya, T; Suzuki, K [Suntory Flowers Ltd., Higashiomi (Japan)

    2005-07-01

    Full text: Garden verbenas (Verbena hybrida) belonging to the Verbenaceae family are originated from interspecific hybridization among several species, many cultivars frequently produce seeds. Recently, a sterile mutant has been isolated in the verbena cultivar 'Coral Pink' of Temari series (Suntory Flowers Ltd., Tokyo, Japan) by mutation induction using heavy-ion beams at RIKEN Accelarator Research Facility (RARF, Saitama, Japan). We investigated pollen and ovule fertility assessed by acetocarmin staining, seed-set following controlled-pollination tests and behavior of pollen tubes in pistils with the sterile mutant of 'Coral Pink' (SC) and its original fertile one (FC). As the results, although SC has functional male and female gametes, few self-pollinated flowers carry out seed-set, leading to sterile phenotype. Additionally, the sterile mechanism of SC was compared with the wild species V. peruviana (VP) which is one of origin of Temari series and exhibits sterility. Interestingly, similar phenotype was observed in PV. We further investigated, therefore, whether there are differences on self-incompatible reaction between SC and VP. (author)

  8. Sterile mutant of Verbena hybrida induced by heavy ion beam irradiation and wild species V. peruviana exhibit self-incompatible phenotype

    International Nuclear Information System (INIS)

    Saito, H.; Hayashi, Y.; Abe, T.; Kanaya, T.; Suzuki, K.

    2005-01-01

    Full text: Garden verbenas (Verbena hybrida) belonging to the Verbenaceae family are originated from interspecific hybridization among several species, many cultivars frequently produce seeds. Recently, a sterile mutant has been isolated in the verbena cultivar 'Coral Pink' of Temari series (Suntory Flowers Ltd., Tokyo, Japan) by mutation induction using heavy-ion beams at RIKEN Accelarator Research Facility (RARF, Saitama, Japan). We investigated pollen and ovule fertility assessed by acetocarmin staining, seed-set following controlled-pollination tests and behavior of pollen tubes in pistils with the sterile mutant of 'Coral Pink' (SC) and its original fertile one (FC). As the results, although SC has functional male and female gametes, few self-pollinated flowers carry out seed-set, leading to sterile phenotype. Additionally, the sterile mechanism of SC was compared with the wild species V. peruviana (VP) which is one of origin of Temari series and exhibits sterility. Interestingly, similar phenotype was observed in PV. We further investigated, therefore, whether there are differences on self-incompatible reaction between SC and VP. (author)

  9. Avaliação de plantas transgênicas de Petunia x hybrida contendo o gene Psag12 - ipt cultivadas sob deficiência nutricional.

    Directory of Open Access Journals (Sweden)

    Francine Lorena Cuquel

    2001-05-01

    Full Text Available Deficiência nutricional em plantas pode causar clorose, necrose, desfolha, redução do crescimento e da produtividade e senescência prematura. Aplicações xógenas de citocinina podem aliviar tais sintomas. Este estudo foi efetuado para avaliar os efeitos da produção auto-regulada de citocinina na tolerância de plantas de petúnia (Petunia x hybrida à deficiência nutricional. Um gene quimérico contendo o promotor SAG12 unido ao gene ipt, o qual codifica a produção de isopentenyl transferase, uma enzima da rota metabólica da biossíntese de citocinina, foi introduzido em petúnia através de transformação mediada por Agrobacterium. Duas plantas contendo o gene ipt foram selecionadas e autopolinizadas para obtenção das linhas a serem submetidas à deficiência nutricional. Ambas as linhas PSAG12–ipt avaliadas foram mais tolerantes à deficiência nutricional que as plantas do tipo selvagem. Os resultados indicam que a produção endógena de citocinina pode aumentar a tolerância das plantas à deficiência nutricional.

  10. Overexpression of PhEXPA1 increases cell size, modifies cell wall polymer composition and affects the timing of axillary meristem development in Petunia hybrida.

    Science.gov (United States)

    Zenoni, Sara; Fasoli, Marianna; Tornielli, Giovanni Battista; Dal Santo, Silvia; Sanson, Andrea; de Groot, Peter; Sordo, Sara; Citterio, Sandra; Monti, Francesca; Pezzotti, Mario

    2011-08-01

    • Expansins are cell wall proteins required for cell enlargement and cell wall loosening during many developmental processes. The involvement of the Petunia hybrida expansin A1 (PhEXPA1) gene in cell expansion, the control of organ size and cell wall polysaccharide composition was investigated by overexpressing PhEXPA1 in petunia plants. • PhEXPA1 promoter activity was evaluated using a promoter-GUS assay and the protein's subcellular localization was established by expressing a PhEXPA1-GFP fusion protein. PhEXPA1 was overexpressed in transgenic plants using the cauliflower mosaic virus (CaMV) 35S promoter. Fourier transform infrared (FTIR) and chemical analysis were used for the quantitative analysis of cell wall polymers. • The GUS and GFP assays demonstrated that PhEXPA1 is present in the cell walls of expanding tissues. The constitutive overexpression of PhEXPA1 significantly affected expansin activity and organ size, leading to changes in the architecture of petunia plants by initiating premature axillary meristem outgrowth. Moreover, a significant change in cell wall polymer composition in the petal limbs of transgenic plants was observed. • These results support a role for expansins in the determination of organ shape, in lateral branching, and in the variation of cell wall polymer composition, probably reflecting a complex role in cell wall metabolism. © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

  11. Small RNA analysis in Petunia hybrida identifies unusual tissue-specific expression patterns of conserved miRNAs and of a 24mer RNA

    Science.gov (United States)

    Tedder, Philip; Zubko, Elena; Westhead, David R.; Meyer, Peter

    2009-01-01

    Two pools of small RNAs were cloned from inflorescences of Petunia hybrida using a 5′-ligation dependent and a 5′-ligation independent approach. The two libraries were integrated into a public website that allows the screening of individual sequences against 359,769 unique clones. The library contains 15 clones with 100% identity and 53 clones with one mismatch to miRNAs described for other plant species. For two conserved miRNAs, miR159 and miR390, we find clear differences in tissue-specific distribution, compared with other species. This shows that evolutionary conservation of miRNA sequences does not necessarily include a conservation of the miRNA expression profile. Almost 60% of all clones in the database are 24-nucleotide clones. In accordance with the role of 24mers in marking repetitive regions, we find them distributed across retroviral and transposable element sequences but other 24mers map to promoter regions and to different transcript regions. For one target region we observe tissue-specific variation of matching 24mers, which demonstrates that, as for 21mers, 24mer concentrations are not necessarily identical in different tissues. Asymmetric distribution of a putative novel miRNA in the two libraries suggests that the cloning method can be selective for the representation of certain small RNAs in a collection. PMID:19369427

  12. Calcium in pollen-pistil interaction in 'Petunia hybrida Hat'. Pt. 3. Localization of Ca2+ ions and Ca2+-ATPase in pollinated pistil

    International Nuclear Information System (INIS)

    Bednarska, E.; Butowt, R.

    1995-01-01

    Studies were carried out of Ca 2+ and Ca 2+ -ATPase localization in pollinated (6 and 48 h after pollination) pistils of 'Petunia hybrida'. The results were confronted with Ca 2+ localization in mature pollen grain and in unpollinated pistil. It has been found that after pollination the number of Ca 2+ sequestered in the stigmal exudate and in the sporoderm of the pollen grain gets lower. That phenomenon was associated with the appearance of a large number of Sb/Ca precipitates in the submembrane cytoplasm of the germinating pollen. In the vacuolized pollen grain, i.e. grown into a pollen tube, there were only a few precipitates. In the pollen tube, Ca 2+ were found in the organelles of the tip cytoplasm and in the external pectin cell wall. Studies with the use of 45 Ca 2+ have revealed that the source of calcium ions incorporated into the pollen tube tip and its pectin wall is the transmitting tract of the style. In the transmitting tract overgrown with pollen tubes, Ca 2+ were localized in the intercellular matrix and in the transmitting cell. Sb/Ca precipitates occurred in the nuclei, around the secretary vesicles and on the plasmalemma in the transverse walls region. Elevated Ca 2+ level was found in degenerating cells (inhibited pollen tubes, transmitting cells, nucellar cells). The progressing degeneration process of the cells of the transmitting tract of the pollinated pistil was associated with a decrease in the activity of plasmalemma Ca 2+ -ATPase. (author). 30 refs, 19 figs

  13. Electrostatic potentials of the S-locus F-box proteins contribute to the pollen S specificity in self-incompatibility in Petunia hybrida.

    Science.gov (United States)

    Li, Junhui; Zhang, Yue; Song, Yanzhai; Zhang, Hui; Fan, Jiangbo; Li, Qun; Zhang, Dongfen; Xue, Yongbiao

    2017-01-01

    Self-incompatibility (SI) is a self/non-self discrimination system found widely in angiosperms and, in many species, is controlled by a single polymorphic S-locus. In the Solanaceae, Rosaceae and Plantaginaceae, the S-locus encodes a single S-RNase and a cluster of S-locus F-box (SLF) proteins to control the pistil and pollen expression of SI, respectively. Previous studies have shown that their cytosolic interactions determine their recognition specificity, but the physical force between their interactions remains unclear. In this study, we show that the electrostatic potentials of SLF contribute to the pollen S specificity through a physical mechanism of 'like charges repel and unlike charges attract' between SLFs and S-RNases in Petunia hybrida. Strikingly, the alteration of a single C-terminal amino acid of SLF reversed its surface electrostatic potentials and subsequently the pollen S specificity. Collectively, our results reveal that the electrostatic potentials act as a major physical force between cytosolic SLFs and S-RNases, providing a mechanistic insight into the self/non-self discrimination between cytosolic proteins in angiosperms. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.

  14. Alcohol and CV Health: Jekyll and Hyde J-Curves.

    Science.gov (United States)

    O'Keefe, Evan L; DiNicolantonio, James J; O'Keefe, James H; Lavie, Carl J

    2018-02-16

    A routine of light or moderate alcohol consumption (≤1 drink/day for women and 1 to 2 drinks/day for men) is associated with a lower risk for all-cause mortality, coronary artery disease (CAD), type 2 diabetes mellitus (T2D), heart failure (HF), and stroke. Conversely, heavy drinking, (>4 drinks/day) is associated with an increased risk for death and cardiovascular (CV) disease (CVD). Excessive alcohol intake trails behind only smoking and obesity among the 3 leading causes of premature deaths in the United States (US). Heavy alcohol use is a common cause of reversible hypertension (HTN), nonischemic dilated cardiomyopathy, atrial fibrillation (AF), and stroke (both ischemic and hemorrhagic). Among males aged 15 to 59 years, alcohol abuse is perhaps the leading cause of premature death. As such, the risk-to-benefit ratio of drinking is less favorable in younger individuals. A daily habit of light to moderate drinking is ideal for those who choose to consume alcohol regularly. Red wine in particular before or during the evening meal is linked with the best long-term CV outcomes. Most of the studies on alcohol and health are observational, and correlation does not prove causation. Health care professionals should not advise nondrinkers to begin drinking because of the paucity of randomized outcome data coupled with the potential for alcohol abuse even among seemingly low risk individuals. Copyright © 2017. Published by Elsevier Inc.

  15. Resistance selection on banana CV. Ambon Kuning Against Fusarium Wilt

    International Nuclear Information System (INIS)

    Sutarto, Ismiyarti; Meldia, Yeni; Jumjunidang

    1998-01-01

    This research was conducted in order to study the occurrence of mutation on irradiated plantlets and their resistance of plants of banana cv. Ambon Kuning against Fusarium wilt. Plantlets of banana cv. Ambon Kuning sized 5 cm were exposed to gamma rays at the doses 5 - 35 Gy intervals, then were subcultured for obtained M 1 V 5 plantlets. More over, the planlets were acclimatized and were planted in the field was already infected by Fasarium (f).culbense (FOC). The result indicated that irradiated plantlets of the doses 20 - 35 Gy were not able to survive up to 6 months after exposing to gamma rays. Abnormalities of M 1 V 5 plantlets originated from irradiated plantlets at the doses 10 and 15 Gy were shown on rossette plantlets with rigid and dark green leaves, and the formation of smooth mass morphologically shaped like calculus. The appearance of plant height and number of suckers of suckers of M 1 V 5 plants in the field was quite various. The number of survival plants after 8 moths planting was 8, 7, 15, and 28, respectively originated from untreated plants and irradiated plantlets at the doses 5, 10, and 15 Gy. After one year planting , only 2 plants were able to survive from irradiated plantlet at the dose 15 Gy. The plants could produce 27 plantlets obtained from culturing their shoot tips. Further study of these plantlets was needed in order create the stability of their resistance to FOC. (author)

  16. EN-CV during LS1: upgrade, consolidation, maintenance, operation

    International Nuclear Information System (INIS)

    Nonis, M.

    2012-01-01

    The Cooling and Ventilation (CV) Group in the Engineering Department (EN) will be heavily involved in several projects and activities during the long shutdown in 2013 and 2014 (LS1) within a time-frame limited to around twelve months. According to the requests received so far, most projects are related to the upgrade of users' equipment, consolidation work, and the construction of new plants. However, through the experience gained from the first years of the LHC run, some projects are also needed to adapt the existing installations to the new operating parameters. Some of these projects are presented hereafter, outlining the impact that they will have on operational working conditions or risks of breakdown. Among these projects we find: the PM32 raising pumps, the cooling of the CERN Control Center, R2E, the backup cooling towers for ATLAS and cryogenics, a thermosyphon for ATLAS, or new pumps in UWs. Finally, EN-CV activities during LS1 for maintenance, operation, and commissioning will be mentioned since they represent a major workload for the Group

  17. Perancangan Kemasan Transportasi Buah Jambu Air (Syzygium aqueum cv Camplong

    Directory of Open Access Journals (Sweden)

    Iswahyudi

    2015-04-01

    Full Text Available Jamboo cv Camplong was an exotic fruit from Sampang Indonesia which had high economic values. The quality of fresh Jamboo was greatly influenced by the types of packaging and ways of its transportation that affected its shelf life. The purpose of this research were designing a primary packaging and analyzing the quality of Jamboo cv Camplong after short transportation (from Sampang to Surabaya. Farmers used conventional packaging with capacity of 8.4 kg/box (dimension 478 mm x 146 mm x 354 mm. Based on theoretical packaging design showed that the flute BC cardboard (capacity 4.5 kg/box, dimension 357 mm x 217 mm x 216 mm with partition flute A cardboard. In fact, there was needed modification of the dimension (342 mm x 210 mm x 200 mm because of the fruits diameter (60-65 mm. This result did not change the efficiency usage of transportation space (91-95% and compression strength of box can support (7 boxes/ stack. The result showed that the mechanical damage after transportation were 20.87% for conventional packaging (as control and 7.70% for modification packaging design (packaging with partition.

  18. Enraizamento de miniestaca caulinar e foliar na propagação vegetativa de cedro-rosa (Cedrela fissilis Vell. Rooting of stem and leaf minicuttings in the vegetative propagation of cedro-rosa (Cedrela fissilis Vell.

    Directory of Open Access Journals (Sweden)

    Aloisio Xavier

    2003-06-01

    Full Text Available No presente trabalho objetivou-se avaliar o enraizamento de cinco diferentes tipos de miniestaca (caulinar, caulinar apical, caulinar intermediária, caulinar apical desfolhada e foliar, na propagação vegetativa de cedro-rosa (Cedrela fissilis por miniestaquia, a partir de material seminal. Os resultados obtidos quanto ao enraizamento indicaram o melhor desempenho da miniestaca caulinar, com 84% de sobrevivência das mudas aos 90 dias de idade, demonstrando o potencial da miniestaquia como alternativa na produção de mudas de cedro-rosa.This work aimed to evaluate the rooting of five different types of minicuttings (stem, apical stem, intermediate stem, apical stem with removed leaves and leaf, in the vegetative propagation of cedro-rosa (Cedrela fissilis, by applying the minicutting technique, using seedlings material. The results showed that stem cutting was the most efficient technique for the vegetative propagation of this species, with up to 84% survival at 90 days of age. Thus, this technique can be used as an alternative in the production of minicuttings of this species, throughout the year.

  19. Forma literária e crítica da lógica racionalista em Guimarães Rosa = Literary form and critique of rationalism in Guimarães Rosa

    Directory of Open Access Journals (Sweden)

    Hansen, João Adolfo

    2012-01-01

    Full Text Available Guimarães Rosa relaciona a sua arte com a tradição literária brasileira e universal como autor de um ato que é simultaneamente estético e social. Essa relação é expressa através de uma forma literária que congrega a representação do sertão, uma referência principal; e a avaliação da mesma a partir de seu ponto de vista particular que é comunicado ao destinatário e ao leitor no estilo dos seus textos. Muitos textos críticos foram produzidos a respeito de Rosa e suas obras, mas uma questão crítica pertinente hoje, depois de muita interpretação de conteúdos, é a do sentido estético e político da intervenção da forma literária de Rosa no cânone

  20. Wilting and biological additive effect on in situ degradability and chemical composition of Arachis pintoi cv Belomonte silage

    OpenAIRE

    Rosana Aparecida Possenti; Evaldo Ferrari Júnior; Valdinei Tadeu Paulino; Ivani Pozar Otsuk; Patrícia Brás

    2010-01-01

    The purpose of this work was to evaluate the effect of wilting and biological additive amendment on chemical composition, fermentation and ruminal degradability of Arachis pintoi cv Belmonte silage. The following treatments were analysed: T1- Arachis pintoi cv Belmonte fresh forage; T2 - Arachis pintoi cv Belmonte fresh forage plus bacterial additive added to the forage prior to the ensilage; T3- Arachis pintoi cv Belmonte wilted by the sun for 4 hours; T4- Arachis pintoi cv Belmonte wilted b...

  1. JOÃO GUIMARÃES ROSA, TRADUTOR DE O ÚLTIMO DOS MAÇARICOS, DE FRED BODSWORTH

    Directory of Open Access Journals (Sweden)

    Pedro Guilherme Bastos Menezes

    2016-12-01

    Full Text Available This article concerns João Guimarães Rosa’s translation of the condensed novel Last of the Curlews (1954, from Canadian writer Fred Bodsworth, entitled O Último dos Maçaricos (1958. In the first place, we describe the context in which the translation was made as well as certain aspects of the process itself, such as the motivation behind it and the use of ornithological books by the translator. Then, some passages of the novel are analyzed in order to corroborate the conceivable effort undertaken by Rosa to write a poetically relevant text, even though the style of the novel does not match that of Rosa’s own oeuvre. Other texts besides the novel are used to achieve these objectives, such as documents, statements from close friends and the correspondence between Rosa and his Italian and German translators.

  2. Analisis Tingkat Kepuasan Kerja Karyawan pada CV Sumber Pangan di Denpasar

    OpenAIRE

    SUPRATMAN, I WAYAN NOVA; USTRIYANA, I NYOMAN GEDE; DEWI, IDA AYU LISTIA

    2016-01-01

    Analysis of Job Satisfaction at CV Food Sources in DenpasarHuman resources is a factor of production which is very important for a company,compared with other factors of production include natural resources, capital, andskills. The purpose of this study was to determine the level of employee satisfactionCV Food Source in Denpasar. The location determination is done deliberately. Thetotal number of the population sampled that stout 40 employees CV Source of Foodin Denpasar. The factors used in...

  3. [The development of pollen grains and formation of pollen tubes in higher plants : I. Quantitative measurements of the DNA-content of generative and vegetative nuclei in the pollen grain and pollen tube of Petunia hybrida mutants].

    Science.gov (United States)

    Hesemann, C U

    1971-01-01

    The DNA-content of generative and vegetative nuclei in mature pollen grains of four Petunia hybrida mutants was determined by cytophotometry. In addition the DNA-content of generative and vegetative nuclei in the pollen tube of two of these four mutants (virescens-2 n and ustulata-2 n) was cytophotometrically measured.The DNA-values found in the generative nuclei indicate that the DNA-replication continues in the mature pollen grain and comes to an end only after the migration of the nuclei into the pollen tube. These data are in disagreement with the results of DNA-measurements described for a limited number of other species which all show completion of DNA-synthesis during the maturation stage of the pollen grains.The vegetative nuclei of the four Petunia mutants studied show significant differences in the onset of the degenerative phase. Extreme variation is manifested in the ustulata-2 n mutant in which the degeneration of nuclei may reach the final stage in the maturing pollen grain. However in this mutant vegetative nuclei with an unaltered DNA-content may also be demonstrated in the pollen tube. Some of the vegetative nuclei in the pollen tube of ustulata-2 n exhibit an increased amount of DNA which could be the result of differential DNA-replication in the vegetative nuclei. The decrease of the DNA-content in a certain fraction of the vegetative nuclei in the maturing pollen grain does not agree with observations made in other species by several authors who report DNA constancy until the pollen grain is fully mature.The data obtained from the analysis of the four Petunia hybrida mutants point to an important role of the vegetative nucleus in the development of the pollen tube. The Petunia hybrida mutants may be regarded as especially favourable material for investigations concerning the function of the vegetative cell in the development of the pollen grain and pollen tube.

  4. Compromising polarity and waveform constraints in focal-mechanism solutions; the Mara Rosa 2010 Mw 4 central Brazil earthquake revisited

    Czech Academy of Sciences Publication Activity Database

    Zahradník, J.; Fojtíková, Lucia; Carvalho, J.; Barros, L.V.; Sokos, E.; Janský, J.

    2015-01-01

    Roč. 63, NOV (2015), s. 323-333 ISSN 0895-9811 R&D Projects: GA ČR GAP210/12/2336 Institutional support: RVO:67985891 Keywords : weak events * focal mechanism * waveforms * polarities * uncertainties * Brazil * Mara Rosa Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 1.737, year: 2015 http://www. science direct.com/ science /article/pii/S0895981115300481

  5. Data report for ROSA-IV LSTF 10% hot leg break experiment Run SB-HL-02

    International Nuclear Information System (INIS)

    Kukita, Yutaka; Hirata, Kazuo; Gotou, Hiroki

    1990-03-01

    Experimental data for the 10% hot leg break test, Run SB-HL-02, conducted at the ROSA-IV Large Scale Test Facility (LSTF) on June 30, 1987, are presented. This test assumed total failure of both high pressure injection (HPI) and auxiliary feedwater (AFW) systems. The test results were characterized by asymmetric loop responses, flashing in the cold legs and upper downcomer, as well as condensation depressurization in the cold legs following injection of emergency core coolant (ECC) from accumulators. (author)

  6. Pengaruh Budaya Organisasi, Kepemimpinan, dan Motivasi Kerja terhadap Kepuasan Kerja Karyawan CV Surya Raya

    Directory of Open Access Journals (Sweden)

    Laksmi Sito Dwi Irvianti

    2012-05-01

    Full Text Available This research was conducted on CV Surya Raya which is engaged in car shipping. The aim of this study is to determine the influence of organization culture, leadership, and working motivation partially to employees’ satisfaction in CV Surya Raya. The data was collected by conducting interviews with the company and distributing questionnaires to 67 employees as respondents and then analyzed using linear regression analysis method. From the research results, leadership variable does not have a significant impact on employees' job satisfaction in CV Surya Raya. Whereas, the motivation variable has greater influence on job satisfaction of CV Surya Raya employees compared to the organization's culture.

  7. BWR 2 % main recirculation line break LOCA tests RUNs 915 and 920 without HPCS in ROSA-III program

    International Nuclear Information System (INIS)

    Suzuki, Mitsuhiro; Nakamura, Hideo; Anoda, Yoshinari; Kumamaru, Hiroshige; Yonomoto, Taisuke; Koizumi, Yasuo; Tasaka, Kanji

    1987-03-01

    This report presents the experimental results of BWR LOCA integral tests, RUNs 915 and 920, which are performed in the ROSA-III program simulating 2 % main recirculation line break LOCA tests with and without pressure control system operation. The ROSA-III test facility simulates a BWR system with volume scale of 1/424 and has four half-length electrically heated fuel bundles, two active recirculation loops, four types of ECCS's, and steam and feedwater systems. The report presents (1) the experimental results of 2 % small break LOCA phanomena in the ROSA-III system and (2) the effects of the pressure control system on the LOCA phenomena. The pressure control system contributed to (A) prevent bulk flashing in the early blowdown phase, (B) early closure of MSIV by L2 level trip, (C) early actuation of ADS by L1 level trip. However, the core thermal responses of the two tests were similar because of the similar mass inventory in PV after the ADS actuation in both tests. (author)

  8. José Rosa y La guaracha del Macho Camacho de Luis Rafael Sánchez: texto, iconotexto y palimpsesto

    Directory of Open Access Journals (Sweden)

    Rita De Maeseneer

    2014-06-01

    Full Text Available La edición de Cátedra de La guaracha del Macho Camacho (2000 de Luis R. Sánchez lleva en su portada una reproducción incompleta de un llamativo cartel del artista puertorriqueño José Rosa. Esta obra, un iconotexto que estudiamos y reproducimos íntegramente, incorpora una rica serie de citas –falsas y verdaderas– de La guaracha. Proponemos un recorrido interpretativo de la configuración visual y textual de esta imagen, creada con otro fin que el de servir de pórtico a la novela. Con ella Rosa condensaba ya en 1977 de manera sorprendente muchos de los enfoques que los críticos literarios de Sánchez sólo desarrollarían mucho más tarde: la desacralización, el enmascaramiento racial, sexual y social, la dictadura del ocio y del consumo, el barroquismo... Como copista infiel y retozón, Rosa reinterpreta La guaracha insertando su autobiografía en ella y sobre todo insistiendo en que oigamos su trasfondo sonoro más profundo hecho de violencia, de racismo, de explotación y de exclusión.

  9. Texture and composition of the Rosa Marina beach sands (Adriatic coast, southern Italy: a sedimentological/ecological approach

    Directory of Open Access Journals (Sweden)

    Moretti Massimo

    2016-06-01

    Full Text Available Beach sands from the Rosa Marina locality (Adriatic coast, southern Italy were analysed mainly microscopically in order to trace the source areas of their lithoclastic and bioclastic components. The main cropping out sedimentary units were also studied with the objective to identify the potential source areas of lithoclasts. This allowed to establish how the various rock units contribute to the formation of beach sands. The analysis of the bioclastic components allows to estimate the actual role of organisms regarding the supply of this material to the beach. Identification of taxa that are present in the beach sands as shell fragments or other remains was carried out at the genus or family level. Ecological investigation of the same beach and the recognition of sub-environments (mainly distinguished on the basis of the nature of the substrate and of the water depth was the key topic that allowed to establish the actual source areas of bioclasts in the Rosa Marina beach sands. The sedimentological analysis (including a physical study of the beach and the calculation of some statistical parameters concerning the grain-size curves shows that the Rosa Marina beach is nowadays subject to erosion.

  10. Comparison studies on catalytic properties of silver nanoparticles biosynthesized via aqueous leaves extract of Hibiscus rosa sinensis and Imperata cylindrica

    Science.gov (United States)

    Fairuzi, Afiza Ahmad; Bonnia, Noor Najmi; Akhir, Rabiatuladawiyah Md.; Akil, Hazizan Md; Yahya, Sabrina M.; Rahman, Norafifah A.

    2018-05-01

    Synthesis of silver nanoparticles has been developed by using aqueous leaves extract (ALE) of Hibiscus rosa sinensis (H. rosa sinensis) and Imperata cylindrica (I. cylindrica). Both plants extract acts as reducing and capping agent. The colour change in reaction mixture (pale yellow to dark brown) was observed during the synthesis process. The formation of silver nanoparticles was confirmed by surface Plasmon Resonance (SPR) at range 300-700 nm for both leaves using UV-Vis Spectroscopy. The reduction of silver ions to silver nanoparticles was completed within 2 hour for H. rosa sinensis and 30 minutes for I. cylindrica extract. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, field emission scanning electron microscope (FESEM) and Fourier transform infrared (FTIR) spectroscopy. The morphology of silver nanoparticles was found to be different when synthesized using different plant extract. In addition, this study also reported on the effect of silver nanoparticles on the degradation of organic dye by sodium borohydride (NaBH4). The silver nanoparticles synthesis by aqueous leaf extract demonstrates rapid, simple and inexpensive method compared to the conventional physical and physical methods. The efficiency of silver nanoparticles as a promising candidate for the catalysis of organic dyes by NaBH4 through the electron transfer is established in the present study.

  11. Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes.

    Science.gov (United States)

    Chu, Van Trung; Weber, Timm; Graf, Robin; Sommermann, Thomas; Petsch, Kerstin; Sack, Ulrike; Volchkov, Pavel; Rajewsky, Klaus; Kühn, Ralf

    2016-01-16

    The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied CRISPR/Cas9 for the knock-in of 8-11 kb inserts into Rosa26 of C57BL/6 zygotes. We found that 10-20 % of live pups derived from microinjected zygotes were founder mutants, without apparent off-target effects, and up to 50 % knock-in embryos were recovered upon coinjection of Cas9 mRNA and protein. Using this approach, we established a new mouse line for the Cre/loxP-dependent expression of Cas9. Altogether, our protocols and resources support the fast and direct generation of new Rosa26 knock-in alleles and of Cas9-mediated in vivo gene editing in the widely used C57BL/6 inbred strain.

  12. CIVAC CV-01 type pressure gage for middle vacuum pumps

    International Nuclear Information System (INIS)

    Olaru, Grigore; Aculai, Agustin

    1997-01-01

    The digital display pressure gage CIVAC CV-01 measures absolute pressures in vacuum range of 10 2 - 10 -3 mbar in any installation or equipment generating or using low pressures. It uses a transducer type PIRANI, model TR-02. It is a portable device, easy to exploit and handle. It is applied in research, chemistry, metallurgical industry, mechanical engineering. The system of coupling the transducer to the enclosure where the pressure is to be measured is of type ISO-PNEUROP with flange, collar and adjusting ring with a DN 25 ring gasket. The technical and functional features are: - Measuring error: ± 35% of real conventional value ; - Response time: 20 ms; - Number of measuring points: 1; - Output signal: 0 - 10 V d.c.; - Repeatability error: 5%; - Max weight: 1,600 Kg; Size: 91 x 117 x 187 mm. (authors)

  13. Cytotoxic diterpenoids from Jatropha curcas cv. nigroviensrugosus CY Yang Roots.

    Science.gov (United States)

    Liu, JieQing; Yang, YuanFeng; Xia, JianJun; Li, XuYang; Li, ZhongRong; Zhou, Lin; Qiu, MingHua

    2015-09-01

    An investigation of phytochemicals from the roots of Jatropha curcas cv. nigroviensrugosus resulted in the isolation of twenty diterpenoids, including lathyranlactone, an unusual diterpenoid lactone possessing a 5/13/3 tricyclic skeleton, jatrocurcasenones A-E and jatrophodiones B-E, as well as 10 known analogues. All isolates were evaluated for cytotoxicity against the HL-60, SMMC-772, A-549, MCF-7 and SW480 human tumor cell lines using the MTS viability assay. Four of the known analogues showed cytotoxic activity in these cell lines, with IC50 values ranging from 2.0 to 23.0 μM. Moreover, the assessment of their cytotoxic structure-activity relationships showed the epoxy ring between C-5 and C-6 and the hydroxyl group at C-2 were the key functionalities for cytotoxicity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Sign Language Translator Application Using OpenCV

    Science.gov (United States)

    Triyono, L.; Pratisto, E. H.; Bawono, S. A. T.; Purnomo, F. A.; Yudhanto, Y.; Raharjo, B.

    2018-03-01

    This research focuses on the development of sign language translator application using OpenCV Android based, this application is based on the difference in color. The author also utilizes Support Machine Learning to predict the label. Results of the research showed that the coordinates of the fingertip search methods can be used to recognize a hand gesture to the conditions contained open arms while to figure gesture with the hand clenched using search methods Hu Moments value. Fingertip methods more resilient in gesture recognition with a higher success rate is 95% on the distance variation is 35 cm and 55 cm and variations of light intensity of approximately 90 lux and 100 lux and light green background plain condition compared with the Hu Moments method with the same parameters and the percentage of success of 40%. While the background of outdoor environment applications still can not be used with a success rate of only 6 managed and the rest failed.

  15. Gamma irradiation studies on Gladiolus cv. White Friendship

    International Nuclear Information System (INIS)

    Banerji, B.K.; Datta, S.K.; Sharma, S.C.

    1994-01-01

    Dormant corms of Gladiolus cv. White Friendship were irradiated with 250, 500, 750, 1000 and 1250 Gy of gamma rays. Reduction in survival, plant height, number of leaves and floret, spike length, leaf and corm size and delayed flowering were recorded after irradiation. Morphological abnormalities in foliage and florets and chromosomal aberration during root tip mitosis increased with increase in exposure. Flowering was ceased cent per cent in highest dose of 1250 Gy. LD 50 on survival basis was found in 750 Gy exposure. MV 2 and MV 3 also followed the similar pattern of results as exhibited by MV 1 plants. Pink flower colour mutation was detected in few plants as sectorial chimeric form in MV 2 in treated population. In MV 3 one plant produced spike with lighter pink florets in 750 Gy treatment. This mutant has been isolated in pure form. (author). 10 refs., 2 figs., 2 tabs

  16. Induced somatic mutation in mango, mangifera indica L. cv. Langra

    International Nuclear Information System (INIS)

    Siddiqui, S.H.

    1985-01-01

    Dormant buds of mango (Mangifera indica L. cv. Langra) exposed to acute gamma-irradiation dosages of 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 kiloroentgens (kR), were grafted on to one-year-old seedling stock. Dosages of 2.0 and 3.0 kR were found satisfactory for the purpose, as measured by bud lethality and scion growth. A bud graft from 3.0 kR bore fruits of excellent quality. Compared with the control, the fruits were heavier, larger and had more creamish-yellow pulp. None of the other morphological changes expressed by the mutant fruits, observed over three fruiting seasons, were disadvantageous. The tree habit is being further investigated before the mutant can be considered for release as an improved cultivar. (author)

  17. Analisis Pengukuran Produktivitas Perusahaan Alsintan CV. Cherry Sarana Agro

    Directory of Open Access Journals (Sweden)

    Prima Fithri

    2016-04-01

    Full Text Available CV Cherry Sarana Agro is a manufacturing company, which produces agricultural machinery. In reality, company evaluatesits work and performance based on level of profits. In order to maintain the company's performance, it is necessary to measures the productivity of company to see how effective the performance of this company. So it can be determined what is needed in order to improve the performance of the company and also can increase profits. Measuring the productivity can be seen from the financial data that obtained from the company. Then based on data processing, show this company's productivity levels decreased during the period of measurement and it means company requires efforts to increase the efficient use of inputs. It is necessary for company to increase its productivity for the future. This improvement was made by taking into account the partial productivity as inputs that affect the profitability of the company on an ongoing basis.

  18. CV activities on the LHC complex during the long shutdown

    CERN Document Server

    Deleval, S; Body, Y; Obrecht, M; Moccia, S; Peon, G

    2011-01-01

    The presentation gives an overview of the major projects and work foreseen to be performed during next long shutdown on cooling and ventilation plants. Several projects are needed following the experience of the last years when LHC was running, in particular the modifications in the water cooling circuits presently in overflow. Some other projects are linked to the CV consolidation plan. Finally, most of the work shall be done to respond to additional requests: SR buildings air conditioning, the need to be able to clean and maintain the LHC cooling towers without a complete stop of cooling circuits, the upgrade of the air conditioning of the CCC rack room cooling etc. For all these activities, the author will detail constraints and the impact on the schedule and on the operation of the plants that will however need to run for most of the shutdown duration. The consequence of postponing the long shutdown from 2012 to 2013 will be also covered.

  19. Propolis extract in postharvest conservation Solo papaya cv. 'Golden'

    Directory of Open Access Journals (Sweden)

    Flávia Regina Passos

    2016-12-01

    Full Text Available The high perishability of papaya (Carica papaya L. reduces its lifespan as well as limits marketing. Coating the fruits is an alternative to aid food preservation. We aimed to evaluate the effects of coating propolis extract on the physicochemical characteristics of papaya stored at room temperature. Solo papayas cv. 'Golden' were randomly divided into five postharvest treatment groups, three forms of dip-coating (70% alcohol, hydroalcoholic extract of propolis to 2.5%, and hydroalcoholic extract of propolis to 5% and two controls (one uncoated and one with refrigerated uncoated fruits. The weight loss, firmness, soluble solids (SS, titratable acidity (TA, SS/TA, and hydrogen potential (pH were evaluated at the intervals of 4 days every 12th storage day. Sensory analysis was performed on the 4th day of storage of papayas and evaluated by untrained through the acceptance testing. Treatments “refrigerated”, “propolis 2.5%”, and “5% propolis” presented with the lowest weight loss. The firmness level for treatment propolis 5% was superior to that of control, alcohol, and 2.5% propolis treatments. The SS was greater for propolis 5% treatment, which only differed from the alcohol treatment. The TA and SS/TA did not vary with the treatments, but with the storage time. The pH of refrigerated papaya showed significant differences in relation to other treatments. The fruits subjected to refrigerated treatment presented with chilling injury. Papayas coated with propolis extract showed sensory acceptability similar to that in other treatments on the 4th day of storage. The coating of propolis extract is a promising alternative for the control of weight loss and for the maintenance of firmness in Solo papaya cv. 'Golden'.

  20. Core Problem: Does the CV Parent Body Magnetization require differentiation?

    Science.gov (United States)

    O'Brien, T.; Tarduno, J. A.; Smirnov, A. V.

    2016-12-01

    Evidence for the presence of past dynamos from magnetic studies of meteorites can provide key information on the nature and evolution of parent bodies. However, the suggestion of a past core dynamo for the CV parent body based on the study of the Allende meteorite has led to a paradox: a core dynamo requires differentiation, evidence for which is missing in the meteorite record. The key parameter used to distinguish core dynamo versus external field mechanisms is absolute field paleointensity, with high values (>>1 μT) favoring the former. Here we explore the fundamental requirements for absolute field intensity measurement in the Allende meteorite: single domain grains that are non-interacting. Magnetic hysteresis and directional data define strong magnetic interactions, negating a standard interpretation of paleointensity measurements in terms of absolute paleofield values. The Allende low field magnetic susceptibility is dominated by magnetite and FeNi grains, whereas the magnetic remanence is carried by an iron sulfide whose remanence-carrying capacity increases with laboratory cycling at constant field values, indicating reordering. The iron sulfide and FeNi grains are in close proximity, providing mineralogical context for interactions. We interpret the magnetization of Allende to record the intense early solar wind with metal-sulfide interactions amplifying the field, giving the false impression of a higher field value in some prior studies. An undifferentiated CV parent body is thus compatible with Allende's magnetization. Early solar wind magnetization should be the null hypothesis for evaluating the source of magnetization for chondrites and other meteorites.

  1. History of a modern place: Clorindo Testa and the Santa Rosa Civic Center, La Pampa

    Directory of Open Access Journals (Sweden)

    Cláudia Costa Cabral

    2013-12-01

    Full Text Available Santa Rosa, capital of the young province of La Pampa, was founded in 1892 at the end of the military campaigns that annihilated the indigenous people and ensured republican control over the Patagonia region. In 1955, the city held a design competition for the construction of its Civic Center. This was not a current infill operation intended to renew the core of the city, since it was not just a matter of raising a new facade along one of the four sides of the main square. The task implied designing, almost from scratch, a new part of city. Competitors were supposed to organize new buildings and public spaces within an area of nine hectares standing between the existing city and the surrounding pampas. Clorindo Testa won the competition by proposing that it be built as a piece of a modern city. Situated in the middle of Argentinean pampas, the Santa Rosa Civic Center has been less extensively discussed than other of Testa’s great contemporary works, such as the London Bank (1959 and the National Library (1962, both in Buenos Aires, even in the South American context. The Government Building, the Bus Station and the covered central space were built before 1963. Testa finished the Legislature Building in 1976, and even though in 2006 he was able to conclude the little Legislature Library, half of the Civic Center area still remains as open space. Nevertheless, as a living piece of the never-completed modern project installed in the far south, La Pampa’s case seems to pose relevant urban questions. This paper explores the case from two complementary perspectives. One focuses on the results of the first competition, recognizing an original contribution to the relationship between modernity, monument, and place. The second discusses the unfinished condition of the Civic Center as constitutive to modern tradition in the very modern sense of the city as a never-completed work.

  2. Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins.

    Science.gov (United States)

    Subburaj, Saminathan; Chung, Sung Jin; Lee, Choongil; Ryu, Seuk-Min; Kim, Duk Hyoung; Kim, Jin-Soo; Bae, Sangsu; Lee, Geung-Joo

    2016-07-01

    Site-directed mutagenesis of nitrate reductase genes using direct delivery of purified Cas9 protein preassembled with guide RNA produces mutations efficiently in Petunia × hybrida protoplast system. The clustered, regularly interspaced, short palindromic repeat (CRISPR)-CRISPR associated endonuclease 9 (CRISPR/Cas9) system has been recently announced as a powerful molecular breeding tool for site-directed mutagenesis in higher plants. Here, we report a site-directed mutagenesis method targeting Petunia nitrate reductase (NR) gene locus. This method could create mutations efficiently using direct delivery of purified Cas9 protein and single guide RNA (sgRNA) into protoplast cells. After transient introduction of RNA-guided endonuclease (RGEN) ribonucleoproteins (RNPs) with different sgRNAs targeting NR genes, mutagenesis at the targeted loci was detected by T7E1 assay and confirmed by targeted deep sequencing. T7E1 assay showed that RGEN RNPs induced site-specific mutations at frequencies ranging from 2.4 to 21 % at four different sites (NR1, 2, 4 and 6) in the PhNR gene locus with average mutation efficiency of 14.9 ± 2.2 %. Targeted deep DNA sequencing revealed mutation rates of 5.3-17.8 % with average mutation rate of 11.5 ± 2 % at the same NR gene target sites in DNA fragments of analyzed protoplast transfectants. Further analysis from targeted deep sequencing showed that the average ratio of deletion to insertion produced collectively by the four NR-RGEN target sites (NR1, 2, 4, and 6) was about 63:37. Our results demonstrated that direct delivery of RGEN RNPs into protoplast cells of Petunia can be exploited as an efficient tool for site-directed mutagenesis of genes or genome editing in plant systems.

  3. Enhanced formation of aromatic amino acids increases fragrance without affecting flower longevity or pigmentation in Petunia × hybrida.

    Science.gov (United States)

    Oliva, Moran; Ovadia, Rinat; Perl, Avichai; Bar, Einat; Lewinsohn, Efraim; Galili, Gad; Oren-Shamir, Michal

    2015-01-01

    Purple Petunia × hybrida V26 plants accumulate fragrant benzenoid-phenylpropanoid molecules and anthocyanin pigments in their petals. These specialized metabolites are synthesized mainly from the aromatic amino acids phenylalanine. Here, we studied the profile of secondary metabolites of petunia plants, expressing a feedback-insensitive bacterial form of 3-deoxy-di-arabino-heptulosonate 7-phosphate synthase enzyme (AroG*) of the shikimate pathway, as a tool to stimulate the conversion of primary to secondary metabolism via the aromatic amino acids. We focused on specialized metabolites contributing to flower showy traits. The presence of AroG* protein led to increased aromatic amino acid levels in the leaves and high phenylalanine levels in the petals. In addition, the AroG* petals accumulated significantly higher levels of fragrant benzenoid-phenylpropanoid volatiles, without affecting the flowers' lifetime. In contrast, AroG* abundance had no effect on flavonoids and anthocyanins levels. The metabolic profile of all five AroG* lines was comparable, even though two lines produced the transgene in the leaves, but not in the petals. This implies that phenylalanine produced in leaves can be transported through the stem to the flowers and serve as a precursor for formation of fragrant metabolites. Dipping cut petunia stems in labelled phenylalanine solution resulted in production of labelled fragrant volatiles in the flowers. This study emphasizes further the potential of this metabolic engineering approach to stimulate the production of specialized metabolites and enhance the quality of various plant organs. Furthermore, transformation of vegetative tissues with AroG* is sufficient for induced production of specialized metabolites in organs such as the flowers. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  4. Calcium in pollen-pistil interaction in `Petunia hybrida Hat`. Pt. 3. Localization of Ca{sup 2+} ions and Ca{sup 2+}-ATPase in pollinated pistil

    Energy Technology Data Exchange (ETDEWEB)

    Bednarska, E.; Butowt, R. [Uniwersytet Mikolaja Kopernika, Torun (Poland)

    1995-12-31

    Studies were carried out of Ca{sup 2+} and Ca{sup 2+}-ATPase localization in pollinated (6 and 48 h after pollination) pistils of `Petunia hybrida`. The results were confronted with Ca{sup 2+} localization in mature pollen grain and in unpollinated pistil. It has been found that after pollination the number of Ca{sup 2+} sequestered in the stigmal exudate and in the sporoderm of the pollen grain gets lower. That phenomenon was associated with the appearance of a large number of Sb/Ca precipitates in the submembrane cytoplasm of the germinating pollen. In the vacuolized pollen grain, i.e. grown into a pollen tube, there were only a few precipitates. In the pollen tube, Ca{sup 2+} were found in the organelles of the tip cytoplasm and in the external pectin cell wall. Studies with the use of {sup 45}Ca{sup 2+} have revealed that the source of calcium ions incorporated into the pollen tube tip and its pectin wall is the transmitting tract of the style. In the transmitting tract overgrown with pollen tubes, Ca{sup 2+} were localized in the intercellular matrix and in the transmitting cell. Sb/Ca precipitates occurred in the nuclei, around the secretary vesicles and on the plasmalemma in the transverse walls region. Elevated Ca{sup 2+} level was found in degenerating cells (inhibited pollen tubes, transmitting cells, nucellar cells). The progressing degeneration process of the cells of the transmitting tract of the pollinated pistil was associated with a decrease in the activity of plasmalemma Ca{sup 2+}-ATPase. (author). 30 refs, 19 figs.

  5. Planning for Integral Development. Public Policies, Economic Growth and Social Improvements in Santa Rosa (Ecuador

    Directory of Open Access Journals (Sweden)

    José Prada-Trigo

    2014-12-01

    Full Text Available Ecuador is immersed, since the adoption of the 2008 Constitution, in a process of economic, social and political changes, following the steps on their path to a more equal, post-extractive and democratic society. The main tool for this purpose, the National Strategy for the «Buen Vivir» (Good living and the Change in the Production Model emphasizes new ways for producing, consumption and self-organization. Although Ecuadorian Constitution comprises these ideas and the Ecuadorian Government has promoted different programs and strategies, there is a limited analysis about the local manifestations of these policies. In this way, this paper proposes a study of the local strategies developed in a medium-size city in the south of Ecuador through the analysis of the local networks and the initiatives of local actors for developing strategies oriented to this end. Interviews and statistical data (demographic, economic and social data mainly are used to confirm the level of accomplishment of these objectives. Thus, the existing local networks and the path of the city of Santa Rosa may explain the different level of recent socioeconomic changes taken place at local level instead of Central government policies.

  6. Core heat transfer analysis during a BWR LOCA simulation experiment at ROSA-III

    International Nuclear Information System (INIS)

    Yonomoto, T.; Koizumi, Y.; Tasaka, K.

    1987-01-01

    The ROSA-III test facility is a 1/424-th volumetrically scaled BWR/6 simulator with an electrically heated core to study the thermal-hydraulic response during a postulated loss-of-coolant accident (LOCA). Heat transfer analyses for 5, 15, 50 and 200% break tests were conducted to understand the basic heat transfer behavior in the core under BWR LOCA conditions and to obtain a data base of post-critical heat flux (CHF) heat transfer coefficients and quench temperature. The results show that the convective heat transfer coefficient of dried-out rods at the core midplane during a steam cooling period is less than approximately 120 W/m 2 K. It is larger than existing data measured at lower pressures during a spray cooling period. Bottom-up quench temperatures are given by a simple equations: The sum of the saturation temperature and a constant of 262 K. Then the heat transfer model in the RELAP4/MOD6/U4/J3 code was revised using the present results. The rod surface temperature behavior in the 200% break test was calculated better by using the revised model although the model is very simple. (orig.)

  7. Rosa damascena Mill. Essential Oil Has Protective Effect Against Testicular Damage in Diabetic Rats.

    Science.gov (United States)

    Hamedi, Somayeh; Shomali, Tahoora; Haghighat, Aliakbar

    2018-05-04

    This study investigates the protective effect of Rosa damascena essential oil on diabetes-induced testicular damage in rats. Thirty-six male Wistar rats were randomly divided into 6 equal groups: Group I: negative control (no treatment); Group II: positive control (diabetic by alloxan injection); Groups III-VI that rendered diabetic and received, respectively, 50, 100, 200, and 400 µg/kg/day rose oil, orally for 28 days. Rose oil did not significantly change body weight and blood glucose level as compared to positive control. Serum testosterone level of rose oil-treated rats remained statistically the same with both negative and positive control groups (Groups I and II). Rats treated with rose oil especially at 2 higher dosages (Groups V and VI) had higher sperm count and increased diameters of seminiferous tubules as compared to Group II. Rose oil even at the lowest dosage significantly increased cell count of spermatogonia, primary spermatocytes, Sertoli cells, and Leydig cells, with better outcomes for higher dosages. It appears that short-term repeated dose administration of rose oil can dose-dependently improve structural deteriorations of testes and epididymal sperm count in diabetic rats.

  8. Post-test analysis of ROSA-III experiment RUNs 705 and 706

    International Nuclear Information System (INIS)

    Koizumi, Yasuo; Soda, Kunihisa; Kikuchi, Osamu; Tasaka, Kanji; Shiba, Masayoshi

    1980-07-01

    The purpose of ROSA-III experiment with a scaled BWR Test facility is to examine primary coolant thermal-hydraulic behavior and performance of ECCS during a postulated loss-of-coolant accident of BWR. The results provide the information for verification and improvement of reactor safety analysis codes. RUNs 705 and 706 assumed a 200% double-ended break at the recirculation pump suction. RUN 705 was an isothermal blowdown test without initial power and initial core flow. In RUN 706 for an average core power and no ECCS, the main steam line and feed water line were isolated immediately on the break. Post-test analysis of RUNs 705 and 706 was made with computer code RELAP4J. The agreement in system pressure between calculation and experiment was satisfactory. However, the calculated heater rod surface temperature were significantly higher than the experimental ones. The calculated axial temperature profile was different in tendency from the experimental one. The calculated mixture level behavior in the core was different from the liquid void distribution observed in experiment. The rapid rise of fuel rod surface temperature was caused by the reduction of heat transfer coefficient attributed to the increase of quality. The need was indicated for improvement of analytical model of void distribution in the core, and also to performe a characteristic test of recirculation line under reverse flow and to examine the core inlet flow rate experimentally and analytically. (author)

  9. Phytoextraction of heavy metals from municipal sewage sludge by Rosa multiflora and Sida hermaphrodita.

    Science.gov (United States)

    Antonkiewicz, Jacek; Kołodziej, Barbara; Bielińska, Elżbieta Jolanta

    2017-04-03

    The aim of the study was to evaluate the efficacy of the multiflora rose var. "Jatar" (Rosa multiflora Thunb. ex Murray) and the Virginia fanpetals (Sida hermaphrodita Rusby) to phytoextract heavy metals from municipal sewage sludge. The 6-year field experiment involved four levels of fertilization with sewage sludge at doses of 0, 10, 20, 40, and 60 Mg DM (Dry Mass) sludge ha -1 . The increasing doses of sewage sludge were found to significantly increase the yield of multiflora rose and Virginia fanpetals biomass. They also significantly increased the content of heavy metals in these plants. The highest uptake of heavy metals by the multiflora rose and Virginia fanpetals crops was recorded at the fertilization dose of 60 Mg DM ⋅ ha -1 . Our investigations show that the Virginia fanpetals was more efficient in the phytoextraction of Cr, Ni, Cu, Zn, and Cd from the sewage sludge than the multiflora rose, due to the greater yields and higher heavy metal uptake by the former plant. In turn, the multiflora rose phytoextracted greater amounts of Pb from the sewage sludge. The analyses indicate that the Virginia fanpetals can be used for phytoremediation (phytoextraction) of heavy metals contained in sewage sludge.

  10. Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil from Rosa indica L. Petals

    Directory of Open Access Journals (Sweden)

    Hafiz Majid Rasheed

    2015-01-01

    Full Text Available Rosa indica L. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester, santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K+ (80 mM than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K+ induced contractions than spontaneous contractions. Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

  11. Immunomodulating pectic polysaccharides from waste rose petals of Rosa damascena Mill.

    Science.gov (United States)

    Slavov, Anton; Kiyohara, Hiroaki; Yamada, Haruki

    2013-08-01

    A water-soluble polysaccharide (RP-1) was obtained from distilled rose petals of Rosa damascena Mill. as an attempt for valorization of the waste. RP-1 showed in vitro intestinal immune system modulating activity through Peyer's patch cells and IL-6 producing activity from macrophages. RP-1 lost most of its immunomodulating activity by degradation of the carbohydrate moiety with periodate. RP-1 was fractionated by anion-exchange and gel filtration chromatography and some of the fractions showed significant intestinal immune system modulating activity. The active fractions were suggested to be pectic polysaccharides and type II arabino-3,6-galactan from the component sugar analyses and the reactivity with Yariv antigen. When some active fractions were digested with endo α-d-(1→4)-polygalacturonase, highest molecular weight fragments which were considered as rhamnogalacturonan I, showed potent immunomodulating activities. To our knowledge, this is a first report which explores the possibility for utilization of waste rose petals as a source of immunomodulating pectic polysaccharides. Copyright © 2013 Elsevier B.V. All rights reserved.

  12. Estimating floodplain sedimentation in the Laguna de Santa Rosa, Sonoma County, CA

    Science.gov (United States)

    Curtis, Jennifer A.; Flint, Lorraine E.; Hupp, Cliff R.

    2013-01-01

    We present a conceptual and analytical framework for predicting the spatial distribution of floodplain sedimentation for the Laguna de Santa Rosa, Sonoma County, CA. We assess the role of the floodplain as a sink for fine-grained sediment and investigate concerns regarding the potential loss of flood storage capacity due to historic sedimentation. We characterized the spatial distribution of sedimentation during a post-flood survey and developed a spatially distributed sediment deposition potential map that highlights zones of floodplain sedimentation. The sediment deposition potential map, built using raster files that describe the spatial distribution of relevant hydrologic and landscape variables, was calibrated using 2 years of measured overbank sedimentation data and verified using longer-term rates determined using dendrochronology. The calibrated floodplain deposition potential relation was used to estimate an average annual floodplain sedimentation rate (3.6 mm/year) for the ~11 km2 floodplain. This study documents the development of a conceptual model of overbank sedimentation, describes a methodology to estimate the potential for various parts of a floodplain complex to accumulate sediment over time, and provides estimates of short and long-term overbank sedimentation rates that can be used for ecosystem management and prioritization of restoration activities.

  13. Hemidesmus indicus and Hibiscus rosa-sinensis Affect Ischemia Reperfusion Injury in Isolated Rat Hearts

    Directory of Open Access Journals (Sweden)

    Vinoth Kumar Megraj Khandelwal

    2011-01-01

    Full Text Available Hemidesmus indicus (L. R. Br. (HI and Hibiscus rosa-sinensis L. (HRS are widely used traditional medicine. We investigated cardioprotective effects of these plants applied for 15 min at concentrations of 90, 180, and 360 μg/mL in Langendorff-perfused rat hearts prior to 25-min global ischemia/120-min reperfusion (I/R. Functional recovery (left ventricular developed pressure—LVDP, and rate of development of pressure, reperfusion arrhythmias, and infarct size (TTC staining served as the endpoints. A transient increase in LVDP (32%–75% occurred at all concentrations of HI, while coronary flow (CF was significantly increased after HI 180 and 360. Only a moderate increase in LVDP (21% and 55% and a tendency to increase CF was observed at HRS 180 and 360. HI and HRS at 180 and 360 significantly improved postischemic recovery of LVDP. Both the drugs dose-dependently reduced the numbers of ectopic beats and duration of ventricular tachycardia. The size of infarction was significantly decreased by HI 360, while HRS significantly reduced the infarct size at all concentrations in a dose-dependent manner. Thus, it can be concluded that HI might cause vasodilation, positive inotropic effect, and cardioprotection, while HRS might cause these effects at higher concentrations. However, further study is needed to elucidate the exact mechanism of their actions.

  14. Green synthesis of gold and silver nanoparticles using Hibiscus rosa sinensis

    Science.gov (United States)

    Philip, Daizy

    2010-03-01

    Biological synthesis of gold and silver nanoparticles of various shapes using the leaf extract of Hibiscus rosa sinensis is reported. This is a simple, cost-effective, stable for long time and reproducible aqueous room temperature synthesis method to obtain a self-assembly of Au and Ag nanoparticles. The size and shape of Au nanoparticles are modulated by varying the ratio of metal salt and extract in the reaction medium. Variation of pH of the reaction medium gives silver nanoparticles of different shapes. The nanoparticles obtained are characterized by UV-vis, transmission electron microscopy (TEM), X-ray diffraction (XRD) and FTIR spectroscopy. Crystalline nature of the nanoparticles in the fcc structure are confirmed by the peaks in the XRD pattern corresponding to (1 1 1), (2 0 0), (2 2 0) and (3 1 1) planes, bright circular spots in the selected area electron diffraction (SAED) and clear lattice fringes in the high-resolution TEM image. From FTIR spectra it is found that the Au nanoparticles are bound to amine groups and the Ag nanoparticles to carboxylate ion groups.

  15. Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil from Rosa indica L. Petals

    Science.gov (United States)

    Rasheed, Hafiz Majid; Khan, Taous; Wahid, Fazli; Khan, Rasool; Shah, Abdul Jabbar

    2015-01-01

    Rosa indica L. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS) technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester), santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K+ (80 mM) than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K+ induced contractions than spontaneous contractions. Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases. PMID:26357519

  16. Modernidad y posmodernidad en El gran señor de Enrique Rosas Paravicino

    Directory of Open Access Journals (Sweden)

    Miguel Arribasplata Cabanillas

    2015-07-01

    Full Text Available En la novela El Gran Señor de Enrique Rosas Paravicino hay una confluencia de pasado histórico y presente conflictivo, donde el fervor religioso cumple un papel de nucleo integrador. En la sociedad andina ficcionalizada aparece una novedosa galería de tipos sociales, como corresponde a un país, como Perú, en pugna por integrar la modernidad con la tradición cultural. Los Andes se constituyen en el gran escenario de una épica popular, con todo el colorido de sus gentes y el paisaje de su cultura. Todo se mueve en torno a un rasgo que es predominante en la novela: el desplazamiento humano en torno a una devoción en plena Cordillera Oriental: el Cristo de la Nieve Resplandeciente. Aquí el sincretismo religioso da lugar a la presencia de personajes involucrados en el mito, la magia y la tradición oral, a la par que asoman los cambios sociales y políticos impulsados por otros actores embarcados en proyectos mundanos, acordes con ese otro gran mito: el progreso colectivo, en perspectiva al desarrollo nacional.

  17. Polyploidization mechanisms: temperature environment can induce diploid gamete formation in Rosa sp.

    Science.gov (United States)

    Pécrix, Yann; Rallo, Géraldine; Folzer, Hélène; Cigna, Mireille; Gudin, Serge; Le Bris, Manuel

    2011-06-01

    Polyploidy is an important evolutionary phenomenon but the mechanisms by which polyploidy arises still remain underexplored. There may be an environmental component to polyploidization. This study aimed to clarify how temperature may promote diploid gamete formation considered an essential element for sexual polyploidization. First of all, a detailed cytological analysis of microsporogenesis and microgametogenesis was performed to target precisely the key developmental stages which are the most sensitive to temperature. Then, heat-induced modifications in sporad and pollen characteristics were analysed through an exposition of high temperature gradient. Rosa plants are sensitive to high temperatures with a developmental sensitivity window limited to meiosis. Moreover, the range of efficient temperatures is actually narrow. 36 °C at early meiosis led to a decrease in pollen viability, pollen ectexine defects but especially the appearance of numerous diploid pollen grains. They resulted from dyads or triads mainly formed following heat-induced spindle misorientations in telophase II. A high temperature environment has the potential to increase gamete ploidy level. The high frequencies of diplogametes obtained at some extreme temperatures support the hypothesis that polyploidization events could have occurred in adverse conditions and suggest polyploidization facilitating in a global change context.

  18. ROSA-III/971, BWR Rig of Safety Assessment LOCA, Loss of Offsite Power Transient

    International Nuclear Information System (INIS)

    1992-01-01

    1 - Description of test facility: ROSA-III is a 1/124 scaled down test facility with electrically heated core designed to study the response of engineered safety features to loss-of-coolant accidents in in commercial BWR. It consists of the following, fully instrumented subsystems: (a) the pressure vessel with a core simulating four half-length fuel assemblies and control rod; (b) steam line and feed water line, which are independent open loops; (c) coolant recirculation system, which consists of two loops provided with a recirculation pump and two jet pumps in each loop; (d) emergency cooling system, including HPCS, LPCS, LPCI, and ADS. 2 - Description of test: Run 971 simulated a BWR LOSS of off-site power transient. The core scram was assumed to occur at 6 seconds after the transient initiated by the turbine trip. HPCS failure was assumed. After ADS started, the upper half of the core was uncovered by steam. The core was re-flooded by LPCS alone

  19. Chemical Fingerprint Analysis and Quantitative Analysis of Rosa rugosa by UPLC-DAD

    Directory of Open Access Journals (Sweden)

    Sanawar Mansur

    2016-12-01

    Full Text Available A method based on ultra performance liquid chromatography with a diode array detector (UPLC-DAD was developed for quantitative analysis of five active compounds and chemical fingerprint analysis of Rosa rugosa. Ten batches of R. rugosa collected from different plantations in the Xinjiang region of China were used to establish the fingerprint. The feasibility and advantages of the used UPLC fingerprint were verified for its similarity evaluation by systematically comparing chromatograms with professional analytical software recommended by State Food and Drug Administration (SFDA of China. In quantitative analysis, the five compounds showed good regression (R2 = 0.9995 within the test ranges, and the recovery of the method was in the range of 94.2%–103.8%. The similarities of liquid chromatography fingerprints of 10 batches of R. rugosa were more than 0.981. The developed UPLC fingerprint method is simple, reliable, and validated for the quality control and identification of R. rugosa. Additionally, simultaneous quantification of five major bioactive ingredients in the R. rugosa samples was conducted to interpret the consistency of the quality test. The results indicated that the UPLC fingerprint, as a characteristic distinguishing method combining similarity evaluation and quantification analysis, can be successfully used to assess the quality and to identify the authenticity of R. rugosa.

  20. Nutritional value of Rosa spp. l. and Cornus mas l. fruits, as affected by storage conditions

    Directory of Open Access Journals (Sweden)

    Craita-Maria Rosu

    2011-12-01

    Full Text Available A comparison between two storage methods (freezing and drying of fruits, in terms of ascorbic acid, carotenes, total sugar and protein content in Rosa spp. L. species and Cornus mas L. was performed. In the dried rose hips, the major losses were registered at the level of ascorbic acid content (32.04–50.25 %, followed by carotenes (30.85–52.08 %, total sugar (21.57–34.6 % and protein content (21.33–46.89 %. The freezing method resulted in a better preservation of ascorbic acid (only 19.80–29.21 % decrease and total sugar content (3.41–12.94 % increase. In the preserved cornelian cherry fruits, no statistically significant differences were registered between fruits categories, except carotene content, which was dramatically decreased in dried fruits (88.23 %. For cornelian cherry fruits, both storage methods induced a decrease of ascorbic acid and protein contents (57.60 and 46.32 % and an increase of total sugar level (37.60 %.

  1. Anticonvulsant and neuroprotective effects of Rosa damascena hydro-alcoholic extract on rat hippocampus

    Directory of Open Access Journals (Sweden)

    Mansour Homayoun

    2015-04-01

    Full Text Available Objective: Previously, analgesic, hypnotic, and anticonvulsant effects have been suggested for Rosa damascena (R. damascena. In the present study, possible anti-seizure and neuro-protective effects of hydro-alcoholic extract of R. damascena has been investigated after inducing seizures in rats by pentylenetetrazole (PTZ. Materials and Methods: The rats were divided to five groups: (1 Control: received saline, (2 PTZ: 100 mg/kg, i.p., (3 PTZ-Extract 50 mg/kg(PTZ-Ext 50, (4 PTZ- Extract 100 mg/kg(PTZ-Ext 100, and (5 PTZ- Extract 200 mg/kg(PTZ-Ext 200 groups which were treated with 50, 100, and 200 mg/kg respectively of hydro-alcoholic extract of R. damascena for one week before PTZ injection. The animals were examined for electrocorticography (ECoG recording and finally, the brains were removed for histological study. Results: The hydro-alcoholic extract of R. damascena significantly prolonged the latency of seizure attacks and reduced the frequency and amplitude of epileptiform burst discharges induced by PTZ injection. Moreover, all three doses of the extract significantly inhibited production of dark neurons in different regions of the hippocampus in the mentioned animal model. Conclusion: The present study showed that the hydro-alcoholic extract of R. damascena has anticonvulsant and neuroprotective effects. More investigations are needed to be done in order to better understand the responsible compound(s as well as the possible mechanism(s.

  2. VULNERABILITY ASSESSMENT USING LIDAR DATA IN SILANG-STA. ROSA SUBWATERSHED, PHILIPPINES

    Directory of Open Access Journals (Sweden)

    M. A. Bragais

    2016-10-01

    Full Text Available Silang-Sta. Rosa Subwatershed is experiencing rapid urbanization. Its downstream area is already urbanized and the development is moving fast upstream. With the rapid land conversion of pervious to impervious areas and increase frequency of intense rainfall events, the downstream of the watershed is at risk of flood hazard. The widely used freeware HEC-RAS (Hydrologic Engineering Center- River Analysis System model was used to implement the 2D unsteady flow analysis to develop a flood hazard map. The LiDAR derived digital elevation model (DEM with 1m resolution provided detailed terrain that is vital for producing reliable flood extent map that can be used for early warning system. With the detailed information from the simulation like areas to be flooded, the predicted depth and duration, we can now provide specific flood forecasting and mitigation plan even at community level. The methodology of using 2D unsteady flow modelling and high resolution DEM in a watershed can be replicated to other neighbouring watersheds specially those areas that are not yet urbanized so that their development will be guided to be flood hazard resilient. LGUs all over the country will benefit from having a high resolution flood hazard map.

  3. ROSA: Resource-Oriented Service Management Schemes for Web of Things in a Smart Home.

    Science.gov (United States)

    Liao, Chun-Feng; Chen, Peng-Yu

    2017-09-21

    A Pervasive-computing-enriched smart home environment, which contains many embedded and tiny intelligent devices and sensors coordinated by service management mechanisms, is capable of anticipating intentions of occupants and providing appropriate services accordingly. Although there are a wealth of research achievements in recent years, the degree of market acceptance is still low. The main reason is that most of the devices and services in such environments depend on particular platform or technology, making it hard to develop an application by composing the devices or services. Meanwhile, the concept of Web of Things (WoT) is becoming popular recently. Based on WoT, the developers can build applications based on popular web tools or technologies. Consequently, the objective of this paper is to propose a set of novel WoT-driven plug-and-play service management schemes for a smart home called Resource-Oriented Service Administration (ROSA). We have implemented an application prototype, and experiments are performed to show the effectiveness of the proposed approach. The results of this research can be a foundation for realizing the vision of "end user programmable smart environments".

  4. Vulnerability Assessment Using LIDAR Data in Silang-Sta Rosa Subwatershed, Philippines

    Science.gov (United States)

    Bragais, M. A.; Magcale-Macandog, D. B.; Arizapa, J. L.; Manalo, K. M.

    2016-10-01

    Silang-Sta. Rosa Subwatershed is experiencing rapid urbanization. Its downstream area is already urbanized and the development is moving fast upstream. With the rapid land conversion of pervious to impervious areas and increase frequency of intense rainfall events, the downstream of the watershed is at risk of flood hazard. The widely used freeware HEC-RAS (Hydrologic Engineering Center- River Analysis System) model was used to implement the 2D unsteady flow analysis to develop a flood hazard map. The LiDAR derived digital elevation model (DEM) with 1m resolution provided detailed terrain that is vital for producing reliable flood extent map that can be used for early warning system. With the detailed information from the simulation like areas to be flooded, the predicted depth and duration, we can now provide specific flood forecasting and mitigation plan even at community level. The methodology of using 2D unsteady flow modelling and high resolution DEM in a watershed can be replicated to other neighbouring watersheds specially those areas that are not yet urbanized so that their development will be guided to be flood hazard resilient. LGUs all over the country will benefit from having a high resolution flood hazard map.

  5. Ecofeminismo, mujeres y desarrollo sustentable: el caso de la Sierra de Santa Rosa en Guanajuato

    Directory of Open Access Journals (Sweden)

    Abril Saldaña Tejeda

    2015-01-01

    Full Text Available en este artículo se explora la participación de las mujeres en el Programa de Desarrollo Sustentable Sierra de Santa Rosa, en Guanajuato, de 2001 a 2011. Se expone la aportación del ecofeminismo para la noción de desarrollo sustentable, y para entender el vínculo entre la naturaleza y las mujeres. Se argumenta que la historia de la región, en específico el auge de la industria minera durante la Colonia, es un buen punto de partida para entender los problemas ambientales de la sierra y para visualizar el vínculo entre las estructuras que funcionan en detrimento del medio ambiente y de las mujeres. Así como también que dicho programa tuvo un efecto positivo en la participación social de las mujeres. Sin embargo, existen todavía retos importantes que se manifiestan en estructuras ideológicas, que impiden la participación de las mujeres como líderes y no sólo como trabajadoras, en las condiciones de pobreza que obstaculizan un verdadero compromiso con la sustentabilidad.

  6. ROSA: Resource-Oriented Service Management Schemes for Web of Things in a Smart Home

    Directory of Open Access Journals (Sweden)

    Chun-Feng Liao

    2017-09-01

    Full Text Available A Pervasive-computing-enriched smart home environment, which contains many embedded and tiny intelligent devices and sensors coordinated by service management mechanisms, is capable of anticipating intentions of occupants and providing appropriate services accordingly. Although there are a wealth of research achievements in recent years, the degree of market acceptance is still low. The main reason is that most of the devices and services in such environments depend on particular platform or technology, making it hard to develop an application by composing the devices or services. Meanwhile, the concept of Web of Things (WoT is becoming popular recently. Based on WoT, the developers can build applications based on popular web tools or technologies. Consequently, the objective of this paper is to propose a set of novel WoT-driven plug-and-play service management schemes for a smart home called Resource-Oriented Service Administration (ROSA. We have implemented an application prototype, and experiments are performed to show the effectiveness of the proposed approach. The results of this research can be a foundation for realizing the vision of “end user programmable smart environments”.

  7. Good livestock practices in dairy farms in Santa Rosa de Cabal, Risaralda, Colombia

    Directory of Open Access Journals (Sweden)

    Libia Marín A

    2017-05-01

    Full Text Available The objective of the present study was to analyze the degree of compliance of these practices, by evaluation of 99 variables in 21 productive systems in Santa Rosa de Cabal, Risaralda. Only one of the farms complied with the 48 fundamental criteria required and, the degree of compliance of major and minor criteria was insufficient. Only 8 of the farms are certified as free of brucellosis and tuberculosis. The best performing areas are animal welfare (81% and those related to the milking routine (79.1%; On the other hand, the most deficient were the control of veterinary drugs and agricultural products (5.8%, as well as the conditions of the cooling tank (32.6%. Through a Multiple Correspondence Analysis, a significant association was found between variables involved with the guarantee in the safety of the milk, regarding the operation, cleaning and disinfection of equipment that guarantee an adequate temperature. There was also an association between variables involved in the milking procedure and hygienic conditions during the milking procedure, involving both the operator and the animal. A lack of training and awareness about the importance of compliance with many of these criteria was found, this is a fundamental tool to obtain a diagnosis of the current sanitary status, and in which it may be necessary to take into account other variables, not included in the current checklist, for a later implementation and follow-up to improvement plans of the productive systems.

  8. Pressure vessel SBLOCA simulation with trace: application to ISTF (Rosa V) - 151

    International Nuclear Information System (INIS)

    Abella, V.; Gallardo, S.; Verdu, G.

    2010-01-01

    In this work, an overview of the results obtained in the simulation of an Upper Head Small Break Loss-Of-Coolant-Accident (SBLOCA) under the assumption of total failure of High Pressure Injection System (HPIS) in the Large Scale Test Facility (LSTF) is provided. In previous works, an SBLOCA located in the Pressure Vessel (PV) Lower Plenum was simulated with TRACE. In that case, an asymmetrical steam generator secondary-side depressurization was produced as an accident management action at the Steam Generator in loop without pressurizer after the generation of safety injection signal to achieve a determined depressurization rate in the primary system. The new SBLOCA scenario has been simulated and results compared with experimental values, with the purpose of completing the analysis of PV SBLOCA. This study is developed in the frame of the OECD/NEA ROSA Project Test 6-1 (SB-PV-9 in JAEA). Finally, the present paper represents a contribution for the study of safety analysis of vessel SBLOCAs and the assessment of the predictability of thermal-hydraulic codes like TRACE. (authors)

  9. Radiocarbon determination of particulate organic carbon in glacier ice from the Grenzgletscher (Monte Rosa)

    International Nuclear Information System (INIS)

    Steier, P.; Drosg, R.; Kutschera, W.; Wild, E.M.; Fedi, M.; Schock, M.; Wagenbach, D.

    2005-01-01

    Full text: Dating ice cores from cold glaciers via radiocarbon is still an unsolved problem. This work describes our approach towards extraction and AMS radiocarbon dating of the particulate organic carbon (POC) fraction in ice samples at VERA (Vienna Environmental Research Accelerator). First measurements were performed on 1 snow and 11 ice samples from Gorner Glacier and Colle Gnifetti in the Monte Rosa Mountain region (Swiss Alps). The sample masses used were between 0.3 kg and 1.4 kg ice yielding between 18 μg and 307 μg carbon as POC. The carbon contamination introduced during the sample processing varied between 9 μg and 33 μg C and originates mainly from the quartz filters and the rinsing liquids used. Minimum sample sizes for successful graphitization of carbon dioxide in our laboratory have been reduced to less than 10 μg carbon. The background in the graphitization process is approximately 0.5 μg carbon of 40 pMC. Scatter and outliers in the radiocarbon data suggest that presently a single radiocarbon date of glacial POC has limited significance. For the set of 11 ice samples, a calibrated age of 2100 BC to 900 AD (95% confidence level) is obtained. (author)

  10. Comparison of leaf anatomical characteristics of hibiscus rosa-sinensis grown in faisalabad region

    International Nuclear Information System (INIS)

    Noman, A.; Ali, Q.; Mehmood, T.; Iftikhar, T.; Mahmeed, M.

    2014-01-01

    The genetic potential of different plant species to different environmental conditions differ in relation to different physiological, biochemical and anatomical characteristics. Of these varying attributes leaf anatomical characteristics play most important role for the establishment of that cultivar in varied environmental conditions. So, the present study was conducted to assess the inter-cultivar genetic potential of Hibiscus in relation to leaf anatomical characteristics. To fulfill the study requirements Hibiscus rosa-sinensis and its six cultivars (were well adapted to their specific natural habitat) were collected from different locations of district Faisalabad Pakistan that have great environmental changes round the year. Results showed significant variability among cultivars in relation to analyzed anatomical characteristics. Cultivars Lemon shiffon and Wilder's white emerge more promising among others by possessing more epidermal thickness, increased epidermal cell area, high cortical cell area and incremented stomatal density as compared with other cultivars. On the other hand, cultivars Cooperi alba, Mrs. George Davis and Frank green possessed least cortex cell area, lowest xylem region thickness and minimum phloem region thickness respectively. Overall, it can be concluded that anatomical genetic potential has endorsed cultivars Lemon chiffon and Wilder's white with enormous capability to grow well under variable environments. (author)

  11. PRACTICAS MEDICAS, PRACTICAS POLITICAS. ROSAS Y LA «VACUNA INDIGENA»

    Directory of Open Access Journals (Sweden)

    MARÍA SILVIA DI LISCIA

    2011-10-01

    Full Text Available BUENOS AIRES, 1830. Se declara una epidemia de viruela entre los indios «pampas» que están en la ciudad para honrar al Restaurador de las Leyes. Los caciques no disimulan su admiración ante la valentía de Rosas, quien los visita sin alarma ante el contagio y los estimula a vacunarse para eliminar el temible mal. Un número estimado en más de cien indios acepta, entonces, inmunizarse, colocándose, según el testimonio de Parish, la «vacuna a indígenas ». Buenos Aires, 1833. Francisco X. Muñiz, médico de la campaña bonaerense, inicia las investigaciones con las que diez años después se logra el redescubrimiento del cow pox, es decir, la viruela vacuna, llamada por Sarmiento «la vacuna indígena».

  12. How Adequate is your CV? Analyzing French CVs with ReaderBench

    NARCIS (Netherlands)

    Gutu, Gabriel; Dascalu, Mihai; Trausan-Matu, Stefan; Lepoivre, Olivier

    2017-01-01

    This study is aimed at presenting a new ReaderBench-based tool built to support candidates in increasing the quality of their CV for a job opening. Both the visual quality and the textual content are considered while also providing an overview and corresponding feedback for the entire CV.

  13. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  14. PERUMUSAN STRATEGI BERSAING JAHE INSTAN PRODUK CV. INTRAFOOD SURAKARTA MENGGUNAKAN PERCEPTUAL MAPPING

    Directory of Open Access Journals (Sweden)

    Mohd. Harisudin

    2013-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui posisi bersaing jahe instan produk CV. Intrafood dan strategi bersaing yang dapat direkomendasikan untuk meraih keberasilannya. Metode dasar yang digunakan adalah analisis deskriptif. Penentuan lokasi penelitian ditentukan dengan metode purposive, yaitu CV. Intrafood Surakarta. Jenis data yang digunakan dalam penelitian ini adalah data primer dan data sekunder. Metode analisis data menggunakan analisis Perceptual Mapping. Dari hasil penelitian diperoleh informasi bahwa jahe instan produk CV Intrafood berada pada peringkat ke-2 dari produk empat jahe instan yang diperbandingkan. Atribut yang dapat dijadikan kekuatan utama dalam meningkatkan pemasaran jahe instan produk CV. Intrafood adalah manfaat produk. Sisi yang paling lemah adalah atribut desain kemasan dan kinerja produk dalam kemasan. Abstract This study aims to determine the competitive position of the product instant ginger CV. Intrafood and competitive strategy can be recommended to achieve success. The basic method used is descriptive analysis. Determining the location of the study are determined by purposive method, namely CV. Intrafood Surakarta. Data used in this study is primary data and secondary data. Method analyzed using Perceptual Mapping. From the results of the study concluded that instant ginger of CV Intrafood product ranks second of four instant ginger products are compared. Attributes that can be used as a major force in improving product marketing instant ginger CV. Intrafood is the benefits of the product. The weakest side is the packaging design and performance attributes of the product in the packaging.

  15. ANALISIS KELAYAKAN INVESTASI PENAMBAHAN MESIN FRAIS BARU PADA CV. XYZ

    Directory of Open Access Journals (Sweden)

    Febri Muhammad Rachadian

    2013-04-01

    Full Text Available Perkembangan teknologi sangat pesat sehingga dunia perindustrian harus mengikuti perkembangannya. Penerapan teknologi diharapkan dapat meningkatkan proses produksi sehingga dapat menghasilkan produk yang berkualitas. Pada industri pembuatan mesin cetak di CV XYZ, mesin-mesin yang digunakan telah hampir lebih dari 20 tahun. Ada indikasi bahwa mesin-mesin tersebut sudah lewat umur pakainya. Alternatif yang digunakan selama ini ialah penggantian komponen yang rusak. Peneliti ingin membandingkan alternative tersebut dengan  penambahan mesin frais baru. Untuk itu diperlukan analisis kelayakan investasi penambahan mesin baru. Dalam perkiraan kurun waktu 10 tahun dan suku bunganya 15 % dengan menggunakan NPV, penambahan mesin frais menarik sebesar Rp 461.201.000 dibandingkan penggantian komponen yang rusak sebesar Rp 211.227.000. Sebaliknya pada PBP, penggantian komponen yang rusak masih mengungguli dengan 1 tahun 4 bulan dibandingkan penambahan mesin frais baru selama 2 tahun 1 bulan. Begitupun juga PI penggantian komponen yang rusak masih menarik sebesar 4,2 dibandingkan PI penambahan mesin frais baru sebesar 2,56. Dapat disimpulkan bahwa dalam jangka waktu 10 tahun, alternatif penggantian komponen yang rusak masih lebih baik dibandingkan penambahan mesin frais baru. Namun, untuk investasi jangka panjang, mungkin saja alternatif penambahan mesin frais baru lebih menarik. Kata Kunci: penggantian komponen rusak, mesin frais baru, mesin cetak Abstract The rapid development of technology makes the world of industry must follow its development. Application of technology is expected to improve the production process so it can be produce a quality product. On the industrial manufacture of printing machines in CV XYZ, the machines have been used almost more than 20 years. There are indications that those machines passed the age lifetime. The alternatives that have been used during this time are the replacement of defective parts. Researchers wanted to

  16. Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L dan Bunga Rosela (Hibiscus sabdariffa L

    Directory of Open Access Journals (Sweden)

    Yusraini Dian Inayati Siregar

    2017-03-01

    Full Text Available Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L dan Bunga Rosela (Hibiscus sabdariffa L telah dilakukan. Penelitian bertujuan untuk mengekstraksi bunga kembang sepatu dan bunga rosella dengan mencari temperatur dan konsentrasi yang optimum untuk mendapatkan pigmen dari bunga kembang sepatu dan bunga rosella dengan pelarut air dan etanol, selain itu dilakukan juga uji stabilitas zat warna. Analisa kadar zat warna dilakukan dengan metode spektrofotometri. Hasil ekstrasi optimum menggunakan metode maserasi dengan pelarut air  adalah pada temperatur 90°C dan dengan pelarut etanol pada konsentrasi 96 %.Uji stabilitas warna memberikan hasil sebagai berikut: a Kondisi penyimpanan, sinar matahari dan sinar lampu dapat mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan meningkatnya nilai absorbansi pada kedua ekstrak. b Penambahan oksidator, H2O2 dapat mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan perubahan dari ekstrak berwarna menjadi ekstrak tidak berwarna karena menghasilkan turunan asam benzoat. c Nilai pH yang semakin meningkat, dari pH 4 ke pH 5, mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan perubahan ekstrak berwarna menjadi tidak berwarna karena terbentuknya basa kuinodal.   Kata Kunci: Ekstraksi, Hibiscus rosa-sinensis L, Hibiscus sabdariffa L, Spektrofotometri UV-Vis

  17. Importancia y propiedades físico química de la Rosa mosqueta (R. canina, R. rubiginosa: una revisión

    Directory of Open Access Journals (Sweden)

    T. Espinoza

    2016-01-01

    Full Text Available Los consumidores cada día exigen productos en sus dietas que no solo aporten los nutrientes requeridos para una vida sana, sino que además son de preferencia aquellos que puedan complementar con propiedades benéficas para la salud. En las últimas décadas se ha reconocido a la Rosa mosqueta, la Rosa canina y a la Rosa rubiginosa, como frutos que contienen muchas propiedades nutracéuticas. El presente trabajo hace una revisión de los artículos de investigación realizados a la Rosa mosqueta desde un punto de vista de su importancia de su consumo y sus beneficios para la salud; describiendo las características químicas, físicas y bioquímicas tales como el contenido de componentes fenólicos, ácidos grasos, ácido linoleico, minerales; y de otros compuestos importantes también desde el punto de vista industrial, tales como el contenido de antioxidantes, pigmentos, entre otros. De acuerdo a los antecedentes recopilados, la Rosa mosqueta es una fuente de micronutrientes incluyendo la vitamina c y el licopeno, entregando además un buen aporte nutricional; por lo anterior debe ser considerado como un alimento funcional. Aun siendo una especie que crece en estado salvaje, sobre suelos forestales; siendo un frutal menor y una planta medicinal su importancia en el consumo masivo debe ser considerada.

  18. A Rosa Mosqueta no tratamento de feridas abertas: uma revisão La Rosa Mosqueta en el tratamiento de heridas abiertas: una revisión Treatment of open wounds using Mosqueta Rose: a review

    Directory of Open Access Journals (Sweden)

    Joyce Silva dos Santos

    2009-06-01

    Full Text Available Trata-se de uma revisão de literatura sobre os principais trabalhos científicos publicados nas últimas duas décadas acerca das propriedades medicinais da Rosa Mosqueta, especialmente no tratamento de feridas abertas. O objetivo é reunir informações que demonstrem, mediante evidências científicas e empíricas, a importância da medicina tradicional quanto ao uso da Rosa Mosqueta em diversas aplicações clínicas. Este estudo caracteriza-se como uma compilação de informações significativas sobre a composição, indicações e usos empíricos do extrato de Rosa Mosqueta, a fim de facilitar pesquisas posteriores, visto que a produção sobre o assunto é escassa. A pesquisa bibliográfica foi realizada através dos bancos de dados Bireme, SciELO, Capes e sites relacionados ao tema na internet.Este articulo es sobre una revisión de la literatura en los principales trabajos científicos publicados en las últimas dos décadas referentes a las características medicinales de Rosa Mosqueta, especialmente en el tratamiento de heridas abiertas. El objetivo es juntar la información que demuestran, con las evidencias científicas y empíricas, la importancia de la medicina tradicional cuánto al uso de Rosa Mosqueta en aplicaciones clínicas diversas. Este estudio se caracteriza como una compilación de la información significativa sobre la composición, las indicaciones y las aplicaciones empíricas del extracto de Rosa Mosqueta, para facilitar la investigación posterior, puesto que la producción en el tema es escasa. La investigación bibliográfica fue llevada a través de las bases de datos Bireme, SciELO, Capes y sitios relacionados con el tema en el Internet.This article is about a literature revision concerning to the main published scientific works in the last two decades about the medicinal properties of the Mosqueta Rose, especially in the treatment of open wounds. The objective is to congregate information demonstrating, by

  19. Nitrogen fertilization on the establishment of Arachis pintoi cv. Belmonte

    Directory of Open Access Journals (Sweden)

    Rita Manuele Porto Sales

    2012-11-01

    Full Text Available The objective was to evaluate the effect of nitrogen fertilization on the establishment of forage peanut (Arachis pintoi cv. Belmonte propagated vegetatively. The experiment was conducted in a greenhouse in a completely randomized design with treatments arranged in a 2 × 4 factorial design - two ages (70 and 85 days after planting and four nitrogen doses (0, 40, 80 and 120 kg/ha - with four replications. Morphogenetic and structural characteristics and production were evaluated. The nitrogen accelerated the establishment of the forage peanut with an increase in dry weight of green leaves and stolons. The greatest length of stolons (48.0 cm was obtained with a dose equivalent to 86 kg N/ha and higher density of stolons (20 stolons/vase between 78 and 82 kg N/ha. Nitrogen fertilization also reduced the phyllochron from 6.7 to 4.6 days/leaf. These data were more intense at 85 days, suggesting greater photosynthetic contribution during this period related to the large number of leaves after 70 days. Therefore, nitrogen can be an important tool to accelerate the establishment of pure stands of forage peanut.

  20. EVALUASI SISTEM INFORMASI PERSEDIAAN PADA CV. SARANA TELEMAXINDO

    Directory of Open Access Journals (Sweden)

    Nelly Nelly

    2010-10-01

    Full Text Available Evaluation objectives of inventory information systems at CV. Facilities Telemaxindo was to evaluate the weaknesses of the implementation of internal control, as well as estimating and assessing possible risks that might arise. The research method used is literature study of reference books in the library, and field study consisted of observation, interviews with relevant parties, creating check lists, check the inventory information systems documentation, and testing of inventory application systems. The results are findings of control weaknesses in the implementation of security management, operational management control, boundary control, input control, and output control on the present inventory information system. These weaknesses could pose a risk and if not dealt soon it could hurt the company. The resulting conclusion of the security management control, boundary control, control input, and output control is not running well so that it needs to be improved in accordance with the recommendations, while operational management control has been running fairly well.Keywords: evaluation, information system, inventory

  1. Infrared small target detection technology based on OpenCV

    Science.gov (United States)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  2. Seven health physics calculator programs for the HP-41CV

    International Nuclear Information System (INIS)

    Rittmann, P.D.

    1984-08-01

    Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices

  3. A virtual computing infrastructure for TS-CV SCADA systems

    CERN Document Server

    Poulsen, S

    2008-01-01

    In modern data centres, it is an emerging trend to operate and manage computers as software components or logical resources and not as physical machines. This technique is known as â€ワvirtualisation” and the new computers are referred to as â€ワvirtual machines” (VMs). Multiple VMs can be consolidated on a single hardware platform and managed in ways that are not possible with physical machines. However, this is not yet widely practiced for control system deployment. In TS-CV, a collection of VMs or a â€ワvirtual infrastructure” is installed since 2005 for SCADA systems, PLC program development, and alarm transmission. This makes it possible to consolidate distributed, heterogeneous operating systems and applications on a limited number of standardised high-performance servers in the Central Control Room (CCR). More generally, virtualisation assists in offering continuous computing services for controls and maintaining performance and assuring quality. Implementing our systems in a vi...

  4. MICROPROPAGAÇÃO DO KIWI CV. HAYWARD

    Directory of Open Access Journals (Sweden)

    SCALIZE FÁBIO EDUARDO

    2001-01-01

    Full Text Available O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch, cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962 -- MS, suplementado com dois tipos de auxina (AIA e AIB e uma citocinina (BAP. Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1, combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1 na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1, independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

  5. Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves

    Science.gov (United States)

    Ganogpichayagrai, Aunyachulee; Palanuvej, Chanida; Ruangrungsi, Nijsiri

    2017-01-01

    Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC50 of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro. PMID:28217550

  6. Produção e qualidade de rosas em três densidades de plantio e uso de soluções conservantes com sacarose na colheita de rosas

    OpenAIRE

    Bastos, Fernanda Espíndola Assumpção

    2014-01-01

    O objetivo deste trabalho foi avaliar a produtividade das cultivares de rosa Avalanche‟ e Carola‟, de coloração branca e vermelha, respectivamente, em três densidades de plantio bem como a qualidade e conservação pós-colheita da cultivar Carola‟, no município de Lages-SC. O experimento referente à densidade de plantio foi implantado em janeiro de 2013, no campus da Universidade do Estado de Santa Catarina (UDESC), com espaçamento de 1,0 m entre fileiras e de 30, 40 e 50 c...

  7. Assembly of the Lactuca sativa, L. cv. Tizian draft genome sequence reveals differences within major resistance complex 1 as compared to the cv. Salinas reference genome.

    Science.gov (United States)

    Verwaaijen, Bart; Wibberg, Daniel; Nelkner, Johanna; Gordin, Miriam; Rupp, Oliver; Winkler, Anika; Bremges, Andreas; Blom, Jochen; Grosch, Rita; Pühler, Alfred; Schlüter, Andreas

    2018-02-10

    Lettuce (Lactuca sativa, L.) is an important annual plant of the family Asteraceae (Compositae). The commercial lettuce cultivar Tizian has been used in various scientific studies investigating the interaction of the plant with phytopathogens or biological control agents. Here, we present the de novo draft genome sequencing and gene prediction for this specific cultivar derived from transcriptome sequence data. The assembled scaffolds amount to a size of 2.22 Gb. Based on RNAseq data, 31,112 transcript isoforms were identified. Functional predictions for these transcripts were determined within the GenDBE annotation platform. Comparison with the cv. Salinas reference genome revealed a high degree of sequence similarity on genome and transcriptome levels, with an average amino acid identity of 99%. Furthermore, it was observed that two large regions are either missing or are highly divergent within the cv. Tizian genome compared to cv. Salinas. One of these regions covers the major resistance complex 1 region of cv. Salinas. The cv. Tizian draft genome sequence provides a valuable resource for future functional and transcriptome analyses focused on this lettuce cultivar. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Performance Comparison of Fuzzy ARTMAP and LDA in Qualitative Classification of Iranian Rosa damascena Essential Oils by an Electronic Nose

    Directory of Open Access Journals (Sweden)

    Abbas Gorji-Chakespari

    2016-05-01

    Full Text Available Quality control of essential oils is an important topic in industrial processing of medicinal and aromatic plants. In this paper, the performance of Fuzzy Adaptive Resonant Theory Map (ARTMAP and linear discriminant analysis (LDA algorithms are compared in the specific task of quality classification of Rosa damascene essential oil samples (one of the most famous and valuable essential oils in the world using an electronic nose (EN system based on seven metal oxide semiconductor (MOS sensors. First, with the aid of a GC-MS analysis, samples of Rosa damascene essential oils were classified into three different categories (low, middle, and high quality, classes C1, C2, and C3, respectively based on the total percent of the most crucial qualitative compounds. An ad-hoc electronic nose (EN system was implemented to sense the samples and acquire signals. Forty-nine features were extracted from the EN sensor matrix (seven parameters to describe each sensor curve response. The extracted features were ordered in relevance by the intra/inter variance criterion (Vr, also known as the Fisher discriminant. A leave-one-out cross validation technique was implemented for estimating the classification accuracy reached by both algorithms. Success rates were calculated using 10, 20, 30, and the entire selected features from the response of the sensor array. The results revealed a maximum classification accuracy of 99% when applying the Fuzzy ARTMAP algorithm and 82% for LDA, using the first 10 features in both cases. Further classification results explained that sub-optimal performance is likely to occur when all the response features are applied. It was found that an electronic nose system employing a Fuzzy ARTMAP classifier could become an accurate, easy, and inexpensive alternative tool for qualitative control in the production of Rosa damascene essential oil.

  9. Evaluation of Biological Effects of Hydroalcoholic Extract of Hibiscus Rosa Sinensis Flowers on Alloxan Induced Diabetes in Rats.

    Science.gov (United States)

    Pethe, Mohan; Yelwatkar, Samir; Manchalwar, Smita; Gujar, Vijay

    2017-08-01

    Aim and Objective The current study sought to investigate antidiabetic, hypolipidimic, antioxidant and histopathological effects of floral extract of Hibiscus rosa sinensis in Alloxan induced Diabetes in rats. Materials and Methods Study was conducted on 6 groups with 6 wistar rats in each group for the period of 4 weeks. Group I: served as normal control (NC), rats administered with gum acacia 1 ml daily, group II: consider as diabetic control (DC) treated with alloxon 150 mg/kg body wt. Whereas Hibiscus rosa-sinensis flower extract was given orally in group III (DE1), group IV (DE2), group V (DE3) at doses of 50, 100 and 200 mg/kg body weight dissolved in distilled water respectively. Group VI (DG) was given glibenclamide (5 mg/kg) as a standard drug and results were compared in reference to it. Results The results indicate that the test compound HEFHR (Hydroalcoholic extract of flower Hibiscus rosa-sinensis) has significant and sustained oral antidiabetic activity, comparable with the hypoglycemic effect of Glibenclamide and Sulphonylurea. Flower extract of HRS was more efficacious in lipid lowering effect and in antioxidative activity than glibenclamide. After 28 day treatment with flower extract, size of islets was significantly increased and necrosis and atrophy of islets were significantly improved; also increase in number and diameter of cell islets appeared to be regular as compared to the diabetic group. Conclusion HEFHR possesses significant antidiabetic, hypolipidemic and antioxidant properties as well as regeneration of beta cells in rats. Further evaluation of HEFHR is in progress. © Georg Thieme Verlag KG Stuttgart · New York.

  10. Aromatherapy with Rosa Damascenes in Apnea, Bradycardia and Spo2 of Preterm Infants; a Randomized Clinical Trial

    Directory of Open Access Journals (Sweden)

    Saeideh Aghagoli

    2016-06-01

    Full Text Available Background  Apnea is one of the most common problems in preterm neonates. This study aimed to evaluate the effectiveness of aromatherapy on the reduction of apnea, bradycardia, and Pulse Oximetry (SPO2 in premature infants. Materials and Methods In a clinical trial study, 60 preterm neonates randomly divided in two groups and exposed to aromatherapy with Rosa damascenes distillate or distilled water beside the routine treatment. In experimental group, two drops of 10% Rosa damascenes distillate was dropped on the pad eye in second day of birth at 6 Am. The intervention was repeated in 9 AM, 12 Am, 15 PM and 18 PM hours. A same condition applied for control group and distilled water was dropped on the pad eye. The number of apnea attacks, bradycardia and decrease in SpO2 compared between two groups using chi-square test, independent t-test and repeated measure test. Results The overall apnea attacks, bradycardia, and SPO2 in tree studied days were lower in intervention group than control group (0.47±0.13 vs. 2.6±0.41, 0.47±0.13 vs. 2.56±0.41 and 0.70±0.17 vs. 2.77±0.21, respectively. In addition, the repeated measurement test showed that the mean number of apnea attacks, decrease in heart pulse rate, and decrease in SpO2 was statistically lower in intervention group with aromatherapy than controls in first, second, third and sum of three days (P< 0.05. Conclusion Aromatherapy with Rosa damascenes distillate can reduce more and speedy the occurrence of apnea attacks, bradycardia and SPO2 in premature infants, along with other routine treatment.

  11. Summary of ROSA-4 LSTF first phase test program and station blackout (TMLB) test results

    International Nuclear Information System (INIS)

    Tasaka, K.; Kukita, Y.; Anoda, Y.

    1990-01-01

    This paper summarizes major test results obtained at the ROSA-4 Large Scale Test Facility (LSTF) during the first phase of the test program. The results from a station blackout (TMLB) test conducted at the end of the first-phase program are described in some detail. The LSTF is an integral test facility being operated by the Japan Atomic Energy Research Institute for simulation of pressurized water reactor (PWR) thermal-hydraulic responses during small-break loss-of-coolant accidents (SBLOCAs) and operational/abnormal transients. It is a 1/48 volumetrically scaled, full-height, full-pressure simulator of a Westinghouse-type 4-loop PWR. The facility includes two symmetric primary loops each one containing an active inverted-U tube steam generator and an active reactor coolant pump. The loop horizontal legs are sized to conserve the scaled (1/24) volumes as well as the length to the square root of the diameter ratio in order to simulate the two-phase flow regime transitions. The primary objective of the LSTF first-phase program was to define the fundamental PWR thermal-hydraulic responses during SBLOCAs and transients. Most of the tests were conducted with simulated component/operator failures, including unavailability of the high pressure injection system and auxiliary feedwater system, as well as operator failure to take corrective actions. The forty-two first phase tests included twenty-nine SBLOCA tests conducted mainly for cold leg breaks, three abnormal transient tests and ten natural circulation tests. Attempts were made in several of the SBLOCA tests to simulate the plant recovery procedures as well as candidate accident management measures for prevention of high-pressure core melt situation. The natural circulation tests simulated the single-phase and two-phase natural circulation as well as reflux condensation behavior in the primary loops in steady or quasi-steady states

  12. Rosa Cappiello’s 'Paese Fortunato' and the Poetics of Alienation

    Directory of Open Access Journals (Sweden)

    Isobel Grave

    2010-05-01

    Full Text Available The paper characterises Rosa Cappiello’s art in Paese fortunato as the poetics of alienation. The narratorprotagonist’s portrayal of the first two years she spent in Sydney took little account of the emerging politics of Australian multiculturalism. Rather, her unselfconscious use of labels that categorise according to ethnicity (‘the Greek girls’/‘those lousy Greek cows’, ‘the Turk’s child’, ‘the pretty Lebanese poofter’, ‘the Yugoslavs’, ‘the French and Danish women’ portrays a deeply divided vision of Australian society. Her isolation is completed by her sense of disconnection from the Italian Australian community, which she detests for what she sees as its crass materialism and its reduction of life to ‘the feverish race for gain’. This suggests that her alienation may not have been the result only of the migration and settlement experience but that it had its roots in her personal pre-migrant life and in deep-seated notions of national identity. With regards to her negative depictions of ethnic characters, we have hypothesised that her sense of alienation was intensified by her critical and literary background. In the second half of the paper, we focus on the author’s experimentation with syntactic categories in order to create effects of fragmentation. Pivotal to the discussion is the tension between physical survival and the genesis of her art, with the discussion then widening to show how Cappiello’s poetics reflect the experiential contours of separation, marginalisation and exile, not only from the perspective of the migrant, but within other sectors of society.

  13. Mapping process and age of Quaternary deposits on Santa Rosa Island, Channel Islands National Park, California

    Science.gov (United States)

    Schmidt, K. M.; Minor, S. A.; Bedford, D.

    2016-12-01

    Employing a geomorphic process-age classification scheme, we mapped the Quaternary surficial geology of Santa Rosa (SRI) within the Channel Islands National Park. This detailed (1:12,000 scale) map represents upland erosional transport processes and alluvial, fluvial, eolian, beach, marine terrace, mass wasting, and mixed depositional processes. Mapping was motivated through an agreement with the National Park Service and is intended to aid natural resource assessments, including post-grazing disturbance recovery and identification of mass wasting and tectonic hazards. We obtained numerous detailed geologic field observations, fossils for faunal identification as age control, and materials for numeric dating. This GPS-located field information provides ground truth for delineating map units and faults using GIS-based datasets- high-resolution (sub-meter) aerial imagery, LiDAR-based DEMs and derivative raster products. Mapped geologic units denote surface processes and Quaternary faults constrain deformation kinematics and rates, which inform models of landscape change. Significant findings include: 1) Flights of older Pleistocene (>120 ka) and possibly Pliocene marine terraces were identified beneath younger alluvial and eolian deposits at elevations as much as 275 m above modern sea level. Such elevated terraces suggest that SRI was a smaller, more submerged island in the late Neogene and (or) early Pleistocene prior to tectonic uplift. 2) Structural and geomorphic observations made along the potentially seismogenic SRI fault indicate a protracted slip history during the late Neogene and Quaternary involving early normal slip, later strike slip, and recent reverse slip. These changes in slip mode explain a marked contrast in island physiography across the fault. 3) Many of the steeper slopes are dramatically stripped of regolith, with exposed bedrock and deeply incised gullies, presumably due effects related to past grazing practices. 4) Surface water presence is

  14. Effect of Antioxidants, Amino Acids and Plant Growth Regulators on in vitro Propagation of Rosa centifolia.

    Science.gov (United States)

    Akhtar, Gulzar; Jaskani, Muhammad Jafar; Sajjad, Yasar; Akram, Ahsan

    2016-03-01

    Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication. The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation. Axillary buds of R. centifolia were sterilized with 70% ethyl alcohol for 4 min and 5% sodium hypochlorite for 2 min followed by three washing with sterilized double distilled water. In order to control oxidative browning, Ascorbic acid (100 mg.L -1 ), citric acid (100 mg.L -1 ) and activated charcoal (3 g.L -1 ) were used while to control withering of shoots, different concentrations (3.0 mg.L -1 , 6.0 mg.L -1 , 9.0 mg.L -1 ) of either glutamine, asparagine and proline were put into trial. Different concentrations of Benzyl aminopurine (BAP) and naphthalene acetic acid (NAA) were used for in vitro shoot and root formation. Minimum browning percentage (20%) was achieved in the presence of activated charcoal (3.0 g.L -1 ) and pretreatment of explants with running tap water. Asparagin (9.0 mg.L -1 ) produced maximum shooting (93%), minimum withering (6.67%), and it took longer period (27 days) for shoots to wither. BAP (3.0 mg.L -1 ) + NAA (0.5 mg.L -1 ) was produced the highest number of shoots (1.63), in a shortest periods (9 days). For root production, NAA (1.5 mg.L -1 ) + BAP (0.5 mg.L -1 ) reduced the time to 11 days with maximum number of roots (4.33) and root length (4.20 cm). The supplement of activated charcoal (3.0 g.L -1 ), a sparagin (9.0 mg.L -1 ) and combination of BAP and NAA in the MS medium is effective for in vitro propagation of R. centifolia.

  15. Estrutura e significado em "Uma rosa para Emily", de William Faulkner

    Directory of Open Access Journals (Sweden)

    Carlos Daghlian

    2004-01-01

    Full Text Available Trata-se de uma análise do consagrado conto "Uma Rosa para Emily", de William Faulkner, voltada para alguns dos principais aspectos de sua estrutura. Após considerarmos o enredo, discutimos a construção das personagens, com destaque para a protagonista, fazendo um levantamento e comentários sobre possíveis fontes de inspiração, destacando, entre outras, aspectos da biografia da poeta Emily Dickinson, a ficção e a poesia de E. A. Poe, romances de Charles Dickens e Henry James, o conto de Sherwood Anderson e a poesia de William Blake, Emily Dickinson, Robert Browning e John Crowe Ransom, acrescentando paralelos com o conto "Bartleby, o escrivão", de Herman Melville. Analisamos, então, o foco narrativo, os símbolos e o significado, ressaltando aqui o desenvolvimento temático da narrativa.This is an analysis of the well-known short story "A Rose for Emily," by William Faulkner, concentrating on some of the main aspects of its structure. A consideration of the plot is followed by a discussion of characterization, with emphasis on the protagonist, by means of a survey and comments on possible sources of inspiration including, among others, aspects of Emily Dickinson's biography, E. A. Poe's fiction and poetry, novels by Charles Dickens and Henry James, Sherwood Anderson's short stories, and the poetry of William Blake, Emily Dickinson, Robert Browning, and John Crowe Ransom, in addition to Herman Melville's short story "Bartleby, the Scrivener." The narrative focus, symbolism and meaning, stressing the thematic development of the narrative, are then analyzed.

  16. Effect of DNA extraction in the Rosa canina L. identification under different processing temperature

    Directory of Open Access Journals (Sweden)

    Jana Žiarovská

    2017-01-01

    Full Text Available Rosa canina, L. is widely used for medicinal purposes as well as in food industry where it is a valuable source, bioactive compounds and natural colorants. Actually, no specific method is recommended as suitable one for DNA extraction from rose hips. The aim of the study was to compare three commercial and three non-commercial methods to extract total genomic DNA from rose hips hyphanthium. Four methods are based on the precipitation in principle and two methods are based on resin-binding. Extracted DNA was proved for the effectivity in following PCR. In total, six different DNA isolations was performed for differently heat processes rose hips - fresh hyphanthium, 2-weeks frozen hyphanthium, dried hyphanthium (50 °C and boiled hyphanthium (100 °C. The amplification parameters of 500 bp chloroplast gene amplicon were evaluated. Obtained amounts of extracted DNA was very variable not only for every individual method used but for individual treatment of samples, too. In general, non-commercial method provided higher amount of extracted DNA, but the A260/280 ratio was lower. When regarding the processing treatment of the samples, high differences were found among the samples untreated by heat and those that were dried or boiled for three of the used extraction methods. All the samples were positive for amplification, but different amounts of amplified product were obtained. The comparison of data for concentrations of extracted DNA and concentrations of amplified product showed large differences when regarding the achieved purity of DNA in extraction.

  17. DIAGNÓSTICO SEROLÓGICO (ROSA DE BENGALA Y MOLECULAR (PCR DE BRUCELOSIS EN HUMANO

    Directory of Open Access Journals (Sweden)

    Orly Fernando Cevallos Falquez

    2010-06-01

    Full Text Available El objetivo de esta investigación fue: Determinar y comparar la presencia de Brucelosis del personal que labora en los camales de los cantones, Buena Fé, Quevedo, El Empalme y Pichincha, utilizando la prueba serológica Rosa de Bengala (RB y la técnica molecular (PCR. Se utilizó como fuente de ADN y anticuerpos sangre periférica. De un total de 115 muestras de sangre recolectadas al personal que labora en los camales como faenadores y operadores, 54 (47% y 15 (13% fueron positivas con RB y PCR respectivamente, dando 61(53% y 100 (87% negativas para las dos pruebas correspondientemente. El promedio de casos positivos para las tres poblaciones excepto Pichincha por PCR fue de 3.4% y para RB 39.4%. Se toma atención con el cantón Pichincha debido a que 19 (70.3% de las muestras salieron positivas con RB de las cuales 12 (44.4% al ser analizadas con PCR fueron negativas, teniendo una correlación de 52.6%, lo que indica una alta incidencia de la enfermedad. Mientras tanto que de las 8 muestras negativas con RB, 2 fueron positivas con PCR dando una correlación entre negativos del 75%. Las muestras positivas amplificaron un fragmento de 725 pb. Con este trabajo se sugiere que la técnica de PCR es una herramienta altamente eficiente y muy útil para el diagnóstico de la brucelosis en humanos.

  18. CRISPR/Cas9-mediated targeting of the Rosa26 locus produces Cre reporter rat strains for monitoring Cre-loxP-mediated lineage tracing.

    Science.gov (United States)

    Ma, Yuanwu; Yu, Lei; Pan, Shuo; Gao, Shan; Chen, Wei; Zhang, Xu; Dong, Wei; Li, Jing; Zhou, Rui; Huang, Lan; Han, Yunlin; Bai, Lin; Zhang, Li; Zhang, Lianfeng

    2017-10-01

    The rat is an important laboratory animal for physiological, toxicological and pharmacological studies. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) is a simple and efficient tool to generate precise genetic modifications in rats, which will promote the accumulation of genetic resources and enable more precise studies of gene function. To monitor Cre-loxP-mediated excision in vivo, we generated a Cre reporter rat strain (Rosa26-imCherry) by knockin of a Cre reporter cassette at the Rosa26 locus using CRISPR/Cas9. Rosa26-imCherry rats exhibited inducible expression of the mCherry cassette (imCherry) using the Cre-loxP system, whereas normal rats exhibited ubiquitous expression of eGFP but not mCherry in the whole body. Injection of adeno-associated virus serotype 9-Cre into the hippocampus and skeletal muscle resulted in mCherry expression in virus-infected cells. Cre-loxP-mediated mCherry expression was then evaluated by crossing Rosa26-imCherry rats with transgenic rats ubiquitously expressing CAG-Cre, heart-specific α-MHC-Cre transgenic rats and liver-specific Alb-Cre knockin rats. Finally, using the established system the expression pattern of Cre driven by two endogenous gene promoters (Wfs1-Cre knockin rat, FabP2-Cre knockin rat) was traced. In summary, we demonstrated excision of the loxP-flanked allele in Rosa26-imCherry rats via activation of mCherry expression in the presence of Cre recombinase. This newly established Rosa26-imCherry rat strain represents a useful tool to facilitate Cre-expression pattern determination and tracing experiments. © 2017 Federation of European Biochemical Societies.

  19. The Effect of Rosa Damascena Extract on Expression of Neurotrophic Factors in the CA1 Neurons of Adult Rat Hippocampus Following Ischemia

    Directory of Open Access Journals (Sweden)

    Seyedeh Farzaneh Moniri

    2018-01-01

    Full Text Available Ischemic stroke is an important cause of death and disability in the world. Brain ischemia causes damage to brain cell, and among brain neurons, pyramidal neurons of the hippocampal CA1 region are more susceptive to ischemic injury. Recent findings suggest that neurotrophic factors protect against ischemic cell death. A dietary component of Rosa damascene extract possibly is associated with expression of neurotrophic factors mRNA following ischemia, so it can have therapeutic effect on cerebral ischemia. The present study attempts to evaluate the neuroprotective effect of Rosa damascene extract on adult rat hippocampal neurons following ischemic brain injury. Forty-eight adult male Wistar rats (weighing 250±20 gr and ages 10-12 weeks used in this study, animals randomly were divided into 6 groups including Control, ischemia/ reperfusion (IR, vehicle and three treated groups (IR+0.5, 1, 2 mg/ml extract. Global ischemia was induced by bilateral common carotid arteries occlusion for 20 minutes. The treatment was done by different doses of Rosa damascena extract for 30 days. After 30 days cell death and gene expression in neurons of the CA1 region of the hippocampus were evaluated by Nissl staining and real time PCR assay. We found a significant decrease in NGF, BDNF and NT3 mRNA expression in neurons of CA1 region of the hippocampus in ischemia group compared to control group (P<0.0001. Our results also revealed that the number of dark neurons significantly increases in ischemia group compared to control group (P<0.0001. Following treatment with Rosa damascene extract reduced the number of dark neurons that was associated with NGF, NT3, and BDNF mRNA expression. All doses level had positive effects, but the most effective dose of Rosa damascena extract was 1 mg/ml. Our results suggest that neuroprotective activity of Rosa damascena can enhance hippocampal CA1 neuronal survival after global ischemia.

  20. ANALISIS PENGUKURAN PRODUKTIVITAS PADA CV. CITRA JEPARA FURNITURE

    Directory of Open Access Journals (Sweden)

    Darminto Pujotomo

    2012-02-01

    Full Text Available CV. Citra Jepara adalah perusahaan furnitur ekspor. Pada masa itu, perusahaan hanya melihat level keuntungan untuk mengkaji perusahaan telah berjalan lebih baik atau kebalikannya. Hasil persepsi membuat perusahaan hanya sedikit memperhatikan efisiensi pemakaian sumber daya yang dimiliki (input sehingga perusahaan membutuhkan biaya besar untuk melakukan aktivitas perusahaan.Penelitian ini mempelajari evaluasi produktivitas perusahaan dan pengukuran serta hubungan antara level produktivitas dengan level profitabilitas (kemampuan perusahaan untuk menciptakan laba yang dapat dicapai oleh perusahaan.Hasil penelitian ini tentang pengukuran produktivitas parsial memperlihatkan level produktivitas berubah-ubah dan masih memerlukan usaha peningkatan produktivitas parsial terutama dalam pemakaian masing-masing input dengan efisien. Level Total Factor Productivity selama perioda pengukuran memperlihatkan level pertumbuhan positif. Ini melukiskan perusahaan sukses dalam mengelola input tenaga kerja dan modal kolektif yang dimiliki. Level Total Productivity selama perioda pengukuran menunjukkan level pertumbuhan yang berubah-ubah. Ini melukiskan perusahaan itu belum dapat mengelola input yang dimiliki keseluruhan dengan sukses. Untuk melukiskan hubungan antara produktivitas total dengan profitabilitas dari hasil penelitian bisa diketahui faktor produktivitas (0,998 mempunyai pengaruh lebih besar dibandingkan pemulihan harga (0,457 untuk peningkatan profitabilitas perusahaan selama kurun waktu satu tahun perioda pengukuran. Diperlukan usaha untuk meningkatkan profitabilitas perusahaan untuk periode mendatang disertai usaha untuk meningkatkan produktivitas yang memusatkan pada peningkatan produktivitas material dan produktivitas modal sebagai input yang sangat mempengaruhi profitabilitas perusahaan dengan cara berkelanjutkan dan berkesinambungan. Kata kunci: pengukuran evaluasi dan produktivitas, profitabilitas    CV. Citra Jepara is an export furniture

  1. Evaluation of Life Table Parameters of Coccinella septempunctata (L. Fed on Macrosiphum rosae (L.

    Directory of Open Access Journals (Sweden)

    Elham Aminafshar

    2016-09-01

    Full Text Available Introduction: Macrosiphum rosae is one of the most important members of the Aphididae (Hemiptera which feeds on plant juices, colonizes underside of leaves,stems and buds. This pest is a polyphagous insect vastly found in Europe, Asia and America. Coccinella septempunctata (L. (Col: Coccinellidae is the successful biological control agent against aphids in under the greenhouse and field conditions. In the present study, the effects of constant temperature on the demography of C. septempunctata which feds on M. rosae was investigated. Materials and Methods: The predators were originally collected from the field (Sorke hesar park in Tehran in march 2012 and reared on aphids in the germinator at 25 ± 1°C, 60 % ± 5 % relative humidity and photoperiod of 16L: 8D/h to lay their eggs and then females were removed after oviposition. These eggs were used for further examination. The life table parameters of C. septempunctata were measured under laboratory conditions at constant temperatures (14, 25 and 31 °C, relative humidity 60 ± 5 % and photoperiod of 16 hours light and 8 hours darkness with 100 repetitions. The different stages of the ladybeetles were fed with 20-100 aphids from different stages of M. rosae. The developmental time of immature stages of beetles were recorded every day. When adult females emerged, an adult male was introduced into the cells to fertilize of them and after that number of offspring produced was counted daily. SAS software v 9.1 was used for data analysis. One-way ANOVA was run to determine the significant differences between demographic parameter of C. septempunctata at two constant temperatures. If there were significant differences, the Tukey's multiple tests were used for multiple comparisons (P < 0.05. Results and Discussion: There was no ovipostion at 14 °C. The intrinsic rate of increase (rm of C. septempunctata at 25 and 31 ˚C were 0.170 and 0.186, the net reproductive rate (R0 were 187.55 and 76.74,the

  2. Prevalencia de Blastocystis sp. y otros protozoarios comensales en individuos de Santa Rosa de Agua, Maracaibo, estado Zulia

    OpenAIRE

    Calchi La Corte, Marinella; Rivero de Rodríguez, Zulbey; Bracho Mora, Angela; Villalobos, Rafael; Acurero de Yamarte, Ellen; Maldonado, Adriana; Chourio-Lozano, Glenis; Díaz, Iris

    2013-01-01

    Con la finalidad de determinar la prevalencia de Blastocystis sp. y especies de protozoarios comensales intestinales del hombre según diferentes aspectos como la edad y el sexo, se evaluaron muestras de heces de 111 individuos varones y mujeres de todas las edades, pobladores de Santa Rosa de Agua, Maracaibo, estado Zulia. Cada muestra fecal se analizó a través de un examen al fresco, tinción de lugol y técnica de concentración de Ritchie. Se utilizó la tinción de hematoxilina férrica para id...

  3. Developmental assessment of RELAP5/MOD3 code against ROSA-IV/TPTF horizontal two-phase flow experiments

    International Nuclear Information System (INIS)

    Kukita, Yutaka; Asaka, Hideaki; Anoda, Yoshinari; Ishiguro, Misako; Tasaka, Kanji; Mimura, Yuichi; Nemoto, Toshiyuki.

    1990-03-01

    A developmental version of the RELAP5/Mod3 code (as of June 1989) was assessed for accuracy using experimental data taken for high-pressure (7MPa) steam-water two-phase flow in a large-diameter (0.18 m) horizontal-pipe test section of the ROSA-IV Two-Phase Flow Test Facility (TPTF). The agreement between the measured and calculated test section void fractions was much better than that for the previous generation of RELAP5 (MOD2). The improvement was achieved primarily due to the code changes with respect to the flow stratification criterion and interfacial-drag calculation scheme. (author)

  4. Data report for ROSA-IV LSTF 10% hot leg break experiment Run SB-HL-04

    International Nuclear Information System (INIS)

    Kukita, Yutaka; Nakamura, Hideo; Saeki, Hiroyuki

    1991-03-01

    Experimental data for the 10% hot leg break test, Run SB-HL-04, conducted on March 29, 1988 at the ROSA-IV Large Scale Test Facility (LSTF), are presented. This test was conducted as part of test series which studied the effect of break orientation on 10% hot leg break transient, and represented a vertical upward break. Other two tests in this test series represented horizontal break and vertical downward break, respectively. The results of these tests were characterized by asymmetric loop responses, flashing in the cold legs as well as upper downcomer, and condensation depressurization in the cold legs following injection of emergency core coolant (ECC) from accumulators. (author)

  5. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking Hibiscus rosa-sinensis L. in Malaysia, with two new country records

    Science.gov (United States)

    Sartiami, Dewi; Watson, Gillian W.; Mohamad Roff, M. N.; Idris, A. B.

    2016-11-01

    A survey of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking the national flower of Malaysia, Hibiscus rosa-sisnensis L. and Hibiscus spp. (Malvaceae) was conducted in Selangor and Kuala Lumpur, Malaysia, from January to March 2016. Adult females were mounted on microscope slides in Canada balsam. The five species identified were Ferrisia dasylirii (Cockerell), Maconellicoccus hirsutus (Green), Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis (Tinsley) and Pseudococcus jackbeardsleyi Gimpel & Miller. Two of these, the invasive species Ferrisia dasylirii and P. solenopsis were introduced and first recorded in Malaysia.

  6. Padrões motores de escolares de oito a nove anos de idade da cidade de Santa Rosa, RS

    OpenAIRE

    Loi, Luiz Serafim de Mello

    1999-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Desportos. O desenvolvimento motor é um assunto que necessita maiores estudos, haja vista a importância cada vez maior do desenvolvimento pleno da criança, desde os primeiros anos de vida. Este estudo utilizando como Marco Referencial Teórico (MRT) o modelo de desenvolvimento motor de Gallahue & Ozmun (1998) teve por objetivo verificar o perfil motor de escolares de oito e nove anos de idade, da cidade de Santa Rosa...

  7. Conversa de bois: uma fábula de João Guimarães Rosa

    OpenAIRE

    Alexandre Veloso de Abreu

    2015-01-01

    Este artigo apresenta o conto “Conversa de Bois” de João Guimaraes Rosa em uma perspectiva fabular. O conceito de fábula abarca um grande espectro de significações, que vai desde: resumo, intriga, conjunto, construção, até o conceito mais formalista, que lhe dá um sentido mais material.  No entanto, imprimiu-se no gênero como característica principal, o cumprimento de uma ação e o uso do antropomorfismo (quando animais assumem características humanas). Na verdade, essa característica é que de...

  8. Novel circulatory connection from the acupoint Zhong Wan(CV12 to pancreas

    Directory of Open Access Journals (Sweden)

    Minsoo Kim

    2008-03-01

    Full Text Available Objectives : Demonstrating a novel circulatory path from the acupoint(CV12 to the pancreas. Method : Alcian blue(1% solution, 20μl, pH 7.4 was injected into the acupoint(CV12. Two hours later the surfaces of internal organs were observed by using a stereomicroscope. Results : Alcian blue arrived and colored the omental fat band(OFB on the pancreas. The OFB connected the head and tail of the pancreas, the pancreas and the spleen, and the pancreas and the stomach. Conclusion : The existence of a novel circulatory path from the acupoint CV12 to the pancreas and its OFB was demonstrated.

  9. Analisis Strategi Mitigasi Resiko Pada Supply Chain CV Surya Cip Dengan House of Risk Model

    OpenAIRE

    Pertiwi, Yoana Ellen; Susanty, Aries

    2017-01-01

    Analysis of Risk Mitigation Strategies on CV Surya CIP's Supply Chain Using House of Risk Model. In the supply chain activities always has the potential of risk, therefore risk management is necessary for handling the risks. At the company that produces plastic molding machine such as CV Surya CIP, in its supply chain activities have a chance to arise risks. The purpose of this study is to identify the various risks that occur in the CV Surya CIP and its causes, severity and determine the ...

  10. Analisis Pengawasan Logistik Produk Aqua Ukuran 330ml Pada CV. Dlu'x Resto Samarinda

    OpenAIRE

    Mardiana, Ali Masuhud, H. Mulyadi Syp

    2016-01-01

    The problem in this research is "Are Determination Against Aqua Products Logistics Control 330ml sizes on CV. DLux Resto has been optimized? "This study aims to determine the amount of inventory on the CV aqua 330ml sizes. Dlu'x Resto in Samarinda.Formulation of the problem in this study is whether the determination of the logistical monitoring product inventory aqua 330ml sizes that have been carried out on the CV. Dlu'x Resto Samarinda already performed optimally.The hypothesis in this stud...

  11. Analisis de exportación y plan estratégico de marketing para la venta de rosas preservadas BEAUTYFLOR en el mercado nacional e internacional

    OpenAIRE

    Moya Cuito, María Gabriela; Almeida Pazmiño, Andrea Carolina

    2016-01-01

    Beautyflor inició sus actividades hace aproximadamente 4 años, con la distribución y venta de rosas naturales preservadas, las mismas que tienen un tiempo de duración de 3 años. El producto se dirigirá a un segmento objetivo en el mercado nacional, el cual comprende, hombres y mujeres que se encuentran entre los 26 a 35 años de edad, quienes, al momento de adquirir rosas preservadas buscan que sus atributos: color, aroma, tamaño y calidad cumplan sus expectativas. Beautyflor, al ser innovador...

  12. The effects of rose hip (Rosa canina) on plasma antioxidative activity and C-reactive protein in patients with rheumatoid arthritis and normal controls: A prospective cohort study

    DEFF Research Database (Denmark)

    Kirkeskov, Bente; Christensen, Robin; Bügel, Susanne Gjedsted

    2011-01-01

    OBJECTIVES: Rose hip (Rosa canina) has been used as an herbal remedy against a wide range of ailments including inflammatory disorders. The anti-inflammatory and antioxidant properties of rose hips have been evaluated in vitro and active constituents have been isolated. Rose hip contains antioxid......OBJECTIVES: Rose hip (Rosa canina) has been used as an herbal remedy against a wide range of ailments including inflammatory disorders. The anti-inflammatory and antioxidant properties of rose hips have been evaluated in vitro and active constituents have been isolated. Rose hip contains...

  13. Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

    OpenAIRE

    Carvalho, Dayse Cristina de; Silva, André Luís Lopes da; Schuck, Mariane Ruzza; Purcino, Marivel; Tanno, Guilherme Nakao; Biasi, Luiz Antonio

    2013-01-01

    The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine wa...

  14. Retention of storage quality and post-refrigeration shelf-life extension of plum (Prunus domestica L.) cv. Santa Rosa using combination of carboxymethyl cellulose (CMC) coating and gamma irradiation

    International Nuclear Information System (INIS)

    Hussain, Peerzada R.; Suradkar, Prashant P.; Wani, Ali M.; Dar, Mohd A.

    2015-01-01

    Carboxymethyl cellulose (CMC) coatings alone and in combination with gamma irradiation was tested for maintaining the storage quality and extending shelf-life of plum. Matured green plums were CMC coated at levels 0.5–1.0% w/v and gamma irradiated at 1.5 kGy. The treated fruit including control was stored under ambient (temperature 25±2 °C, RH 70%) and refrigerated (temperature 3±1 °C, RH 80%) conditions. In fruits treated with individual treatments of 1.0% w/v CMC; 1.5 kGy irradiation and combination of 1.0% w/v CMC and 1.5 kGy irradiation, no decay was recorded up to 11, 17 and 21 days of ambient storage. Irradiation alone at 1.5 kGy gave 8 days extension in shelf-life of plum compared to 5 days by 1.0% w/v CMC coating following 45 days of refrigeration. All combinatory treatments of CMC coating and irradiation proved beneficial in maintaining the storage quality as well as delaying the decaying of plum during post-refrigerated storage at 25±2 °C, RH 70% but, combination of CMC at 1.0% w/v and 1.5 kGy irradiation was found significantly (p≤0.05) superior to all other treatments in maintaining the storage quality and delaying the decaying of plum. CMC coating of plums at 1.0% w/v followed by irradiation at 1.5 kGy resulted in chlorophyll retention of 19.4% after 16 days compared to 10% in control after 8 days of ambient storage. Under refrigerated conditions, same treatment gave retention of 67.6% in chlorophyll compared to 10.6% in control after 35 days of storage. The above combinatory treatment resulted in extension of 11 days in shelf-life of plum during post-refrigerated storage at 25±2 °C, RH 70% following 45 days of refrigeration. Based on microbial analysis, irradiation alone at 1.5 kGy and in combination with 1.0% w/v CMC resulted in 2.0 and 1.8 log reduction in yeast and mold count of plum fruit after 20 and 35 days of ambient and refrigerated storage, thereby ensuring consumer safety. - Highlights: • Irradiation and CMC alone at 1.5 kGy and 1.0% gave 8 and 5 days extension in shelf life. • Treatment of 1% w/v CMC+1.5 kGy inhibited decay up to21 days of ambient storage. • Combination of 1% CMC and 1.5 kGy irradiation extended shelf-life of plum by 11 days. • Combination treatment can help fruit marketing in glut season and enabling good returns

  15. Limited geographic distribution of the novel cyclovirus CyCV-VN.

    Science.gov (United States)

    Le, Van Tan; de Jong, Menno D; Nguyen, Van Kinh; Nguyen, Vu Trung; Taylor, Walter; Wertheim, Heiman F L; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Nguyen, Hanh Uyen; Giri, Abhishek; Nguyen, Thi Thuy Chinh Bkrong; Ho, Dang Trung Nghia; Farrar, Jeremy; Bryant, Juliet E; Tran, Tinh Hien; Nguyen, Van Vinh Chau; van Doorn, H Rogier

    2014-02-05

    A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600 CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease.

  16. Analisis Pengaruh Lingkungan Kerja dan Pemberian Kompensasi terhadap Kinerja Karyawan CV Mum Indonesia

    Directory of Open Access Journals (Sweden)

    Jerry M. Logahan

    2012-05-01

    Full Text Available Mum CV is a company engaged in the sale of bread. To increase sales of bread (performance it needs to investigate the influence of work environment and compensation of employees. The method of analysis used in this study is descriptive, Pearson Regression, and Multiple Regression. The data were obtained from the employees performance by completing the questionnaires provided using Likert scale which was useful to determine the level of disagreement questions on the questionnaires. Results achieved in this study are the work environment has no significant influence on employees performance in CV Mum Indonesia, amounting to 0,068. Compensation has significant, no influence on employees performance in CV Mum Indonesia that is equal to 0,580 and there is a significant effect of 33.6%. Work environment and compensation have a significant impact on employees performance in CV Mum Indonesia. It is equal to 0,580 and there is a significant effect of 33.6%.

  17. Guimarães Rosa e Monteiro Lobato: uma aproximação insólita

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Santilli

    2002-03-01

    Full Text Available Poderá ser surpreendente fazer pendant de escritores, com Guimarães Rosa e Monteiro Lobato. Mas haverá, no mínimo, a procedência de ser ilustrativo do trabalho que aqui se apresenta. Ficou, em nossa memória mais remota de leituras, que as figuras encantadoras do imaginário infanto-juvenil europeu, em sua coorte de significados, desembarcaram, com Lobato, para nacionalizarem-se em terreiro do Brasil. Mas ficou, também, em nossa memória mais recente de leituras, que o pensamento dos antigos ou “clássicos” da Grécia, em seus sedutores achados, pousou no sertão grande de Guimarães Rosa, transfundindose à passagem pelas veredas da metabolização cultural brasileira. Este trabalho tem, então, o objetivo de refletir sobre tal operação, realizada no processo da alquimia literária rosiana, através de recorte estabelecido à volta do conto “A hora e a vez de Augusto Matraga”, de Primeiras estórias. 

  18. Spatial and temporal transcriptome changes occurring during flower opening and senescence of the ephemeral hibiscus flower, Hibiscus rosa-sinensis.

    Science.gov (United States)

    Trivellini, Alice; Cocetta, Giacomo; Hunter, Donald A; Vernieri, Paolo; Ferrante, Antonio

    2016-10-01

    Flowers are complex systems whose vegetative and sexual structures initiate and die in a synchronous manner. The rapidity of this process varies widely in flowers, with some lasting for months while others such as Hibiscus rosa-sinensis survive for only a day. The genetic regulation underlying these differences is unclear. To identify key genes and pathways that coordinate floral organ senescence of ephemeral flowers, we identified transcripts in H. rosa-sinensis floral organs by 454 sequencing. During development, 2053 transcripts increased and 2135 decreased significantly in abundance. The senescence of the flower was associated with increased abundance of many hydrolytic genes, including aspartic and cysteine proteases, vacuolar processing enzymes, and nucleases. Pathway analysis suggested that transcripts altering significantly in abundance were enriched in functions related to cell wall-, aquaporin-, light/circadian clock-, autophagy-, and calcium-related genes. Finding enrichment in light/circadian clock-related genes fits well with the observation that hibiscus floral development is highly synchronized with light and the hypothesis that ageing/senescence of the flower is orchestrated by a molecular clock. Further study of these genes will provide novel insight into how the molecular clock is able to regulate the timing of programmed cell death in tissues. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  19. Hibiscus rosa sinensis Linn. Petals Modulates Glycogen Metabolism and Glucose Homeostasis Signalling Pathway in Streptozotocin-Induced Experimental Diabetes.

    Science.gov (United States)

    Pillai, Sneha S; Mini, S

    2016-03-01

    The prevalence of diabetes mellitus is becoming more and more serious and reaches epidemic proportions worldwide. Scientific research is constantly looking for new agents that could be used as dietary functional ingredients in the fight against diabetes. The objective of the present study was to evaluate the effect of ethyl acetate fraction of Hibiscus rosa sinensis Linn. petals on experimental diabetes at a dose of 25 mg/kg body weight and it was compared with standard anti-diabetic drug metformin. The elevated levels of serum glucose (398.56 ± 35.78) and glycated haemoglobin (12.89 ± 1.89) in diabetic rats were significantly decreased (156.89 ± 14.45 and 6.12 ± 0.49, respectively) by Hibiscus rosa sinensis petals (EHRS) administration. Hepatotoxicity marker enzyme levels in serum were normalized. The fraction supplementation restored the glycogen content by regulating the activities of glycogen metabolizing enzymes. It significantly modulated the expressions of marker genes involved in glucose homeostasis signalling pathway. Histopathological analysis of liver and pancreas supported our findings. The overall effect was comparable with metformin. Hence, our study reveals the role of hibiscus petals for alleviation of diabetes complications, thus it can be propagated as a nutraceutical agent.

  20. Analysis of FAME for two species of earthworms Allolobophora caliginosa Savigny and Pheretima hawayana Rosa (Annelida-Oligochaeta

    Directory of Open Access Journals (Sweden)

    Hossam El-Din Mohamed Omar

    2012-01-01

    Full Text Available Analysis of fatty acid (FA is one of the most commonly used tools for investigating microbial populations in ecological studies. Fatty acids can be extracted and esterified to form fatty acid methyl esters (FAME when analyzed using gas chromatography–mass spectrometry, the resulting profile contains some microbial biomarkers. The aim of the present study to analysis FAME present in two species of earthworms exhibit the same environment. The thin layer chromatography of earthworm Allolobophora caliginosa Savigny showed three major spots corresponding to FAME while that of earthworm Pheretima hawayana Rosa showed two major spots. The GC-MS analysis of Allolobophora caliginosa Savigny extract showed the presence of at least 23 peaks, only two peaks were identified from their Rt and Ms spectrum, hexadecanoic acid methyl ester and octadecenoic acid methyl ester. While the chromatogram of Pheretima hawayana Rosa extract showed at least 20 peaks, five of them were identified; butanedioic acid dimethyl ester, pentadecanoic acid 14-methyl ester, 2-hydroxy-hexadecanoic acid methyl ester, 9-octadecenoic acid methyl ester and octadecanoic acid methyl ester. In conclusion, in spite of the two species of earthworms from the same environment, but their content of FAME was different and that may explain why the biological activity of the whole body extract was different.

  1. BWR 200 % recirculation pump suction line break LOCA tests, RUNs 942 and 943 at ROSA-III without HPCS

    International Nuclear Information System (INIS)

    Suzuki, Mitsuhiro; Tasaka, Kanji; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Yonomoto, Taisuke; Murata, Hideo; Koizumi, Yasuo

    1986-03-01

    This report presents the experimental results of RUNs 942 and 943 in ROSA-III program, which are 200 % recirculation pump suction line break LOCA tests with assumption of HPCS failure. The ROSA-III test facility simulates a BWR system with volume scale of 1/424 and has four half-length electrically heated fuel bundles, two active recirculation loops, ECCS's, and steam and feedwater systems. Effects of initial core void distribution and other fluid conditions on overall LOCA phenomena with special interest on transient core cooling phenomena were investigated by comparing the present test results with those of RUN 926, a 200 % suction line break test with standard initial fluid conditions. The initial core outlet quality was changed between 5 % and 43 %. As conclusions, (1) the initial lower core flow and higher void fraction affected significantly the core cooling conditions and resulted in earlier and higher PCT. (2) The lower plenum flashing temporarily contributed to cool down the core. (3) Flashing of remained hot water in the feedwater line affected slightly the pressure response and delayed the actuation of LPCI by 11 seconds. (4) The whole core was completely cooled down within 104 seconds after the LPCI actuation in these large break tests. (author)

  2. Components in aqueous Hibiscus rosa-sinensis flower extract inhibit in vitro melanoma cell growth

    Directory of Open Access Journals (Sweden)

    Karina H. Goldberg

    2017-01-01

    Full Text Available Skin cancer is extremely common, and melanoma causes about 80% of skin cancer deaths. In fact, melanoma kills over 50 thousand people around the world each year, and these numbers are rising. Clearly, standard treatments are not effectively treating melanoma, and alternative therapies are needed to address this problem. Hibiscus tea has been noted to have medicinal properties, including anticancer effects. Extracts from Hibiscus have been shown to inhibit the growth of a variety of cancer cells. In particular, recent studies found that polyphenols extracted from Hibiscus sabdariffa by organic solvents can inhibit melanoma cell growth. However, effects of aqueous extracts from Hibiscus rosa-sinesis flowers, which are commonly used to make traditional medicinal beverages, have not been examined on melanoma cells. Here, we report that aqueous H. rosa-sinesis flower extract contains compounds that inhibit melanoma cell growth in a dose dependent manner at concentrations that did not affect the growth of nontransformed cells. In addition, these extracts contain low molecular weight growth inhibitory compounds below 3 kD in size that combine with larger compounds to more effectively inhibit melanoma cell growth. Future work should identify these compounds, and evaluate their potential to prevent and treat melanoma and other cancers.

  3. «O Nome da Rosa», romance de transcrição e de metalinguagem

    Directory of Open Access Journals (Sweden)

    Eliana Lourenço de Lima Reis

    2011-10-01

    Full Text Available O trabalho procura estudar O Nome da Rosa sob dois aspectos: como romance de transcrição, focalizando a figura do narrador, e também como metalinguagem. Como se trata de um livro sobre livros, Umberto Eco chama a atenção para o caráter intertextual e fenomênico da escrita. Seu uso das técnicas do romance policial visa ao mesmo tempo distrair o leitor e apresentar um modelo de interpretação tanto de um mundo feito de sinais, quanto do próprio texto. O livro torna-se, então, uma versão ficcional de sua pesquisa sobre semiótica e comunicação.Codesto studio de Il Nome della Rosa focalizza due aspectti: quello del metalinguaggio e quello del romanzo di transcrizione, valorizzando il ruolo del narratore. Sicomme è un libro sui libri, Umberto Eco fa risaltare il carattere intertestuale e fenomenico della scrittura. L'impiego delle tecnica di costruzione del giallo mira a intrattenere il lettore e contemporaneamente offre una chiave interpretativa del testo stesso e di un mondo di segni. Il romanzo si trasforma cosi un una versione fittizia della sua ricerca si semiotica e di comunicazione.

  4. BWR 1 % main recirculation line break LOCA tests, RUNs 917 and 918, without HPCS at ROSA-III program

    International Nuclear Information System (INIS)

    Suzuki, Mitsuhiro; Okazaki, Motoaki; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Yonomoto, Taisuke; Koizumi, Yasuo; Tasaka, Kanji

    1988-07-01

    In a case of small break loss-of-coolant accident (LOCA) at a boiling water reactor (BWR) system, it is important to lower the system pressure to cool down the reactor system by using either the high pressure core spray (HPCS) or the automatic depressurization system (ADS). The report presents characteristic test results of RUNs 918 and 917, which were performed at the rig-of-safety assessment (ROSA)-III program simulating a 1 % break BWR LOCA with an assumption of HPCS failure, and clarifies effects of the ADS delay time on a small break LOCA. The ROSA-III test facility simulates principal components of a BWR/6 system with volumetric scaling factor of 1/424. It is experimentally concluded that the ADS delay time shorter than 4 minutes results in a similar PCT as that in a standard case, in which the PCT is observed after actuation of the low pressure core spray (LPCS). And the ADS delay time longer than 4 minutes results in higher PCT than in the standard case. In the latter, the PCT depends on the ADS time, a 220 K higher PCT, for example, in a case of 10 minutes ADS delay compared with the standard case. (author) 52 refs. 299 figs

  5. ROSA-V large scale test facility (LSTF) system description for the third and fourth simulated fuel assemblies

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Mitsuhiro; Nakamura, Hideo; Ohtsu, Iwao [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment] [and others

    2003-03-01

    The Large Scale Test Facility (LSTF) is a full-height and 1/48 volumetrically scaled test facility of the Japan Atomic Energy Research Institute (JAERI) for system integral experiments simulating the thermal-hydraulic responses at full-pressure conditions of a 1100 MWe-class pressurized water reactor (PWR) during small break loss-of-coolant accidents (SBLOCAs) and other transients. The LSTF can also simulate well a next-generation type PWR such as the AP600 reactor. In the fifth phase of the Rig-of-Safety Assessment (ROSA-V) Program, eighty nine experiments have been conducted at the LSTF with the third simulated fuel assembly until June 2001, and five experiments have been conducted with the newly-installed fourth simulated fuel assembly until December 2002. In the ROSA-V program, various system integral experiments have been conducted to certify effectiveness of both accident management (AM) measures in beyond design basis accidents (BDBAs) and improved safety systems in the next-generation reactors. In addition, various separate-effect tests have been conducted to verify and develop computer codes and analytical models to predict non-homogeneous and multi-dimensional phenomena such as heat transfer across the steam generator U-tubes under the presence of non-condensable gases in both current and next-generation reactors. This report presents detailed information of the LSTF system with the third and fourth simulated fuel assemblies for the aid of experiment planning and analyses of experiment results. (author)

  6. Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV for Diesel Engines

    Directory of Open Access Journals (Sweden)

    Saddam H. Al-lwayzy

    2014-03-01

    Full Text Available This work aims to investigate biofuels for diesel engines produced on a lab-scale from the fresh water microalgae Chlorella vulgaris (FWM-CV. The impact of growing conditions on the properties of biodiesel produced from FWM-CV was evaluated. The properties of FWM-CV biodiesel were found to be within the ASTM standards for biodiesel. Due to the limited amount of biodiesel produced on the lab-scale, the biomass of dry cells of FWM-CV was used to yield emulsified water fuel. The preparation of emulsion fuel with and without FWM-CV cells was conducted using ultrasound to overcome the problems of large size microalgae colonies and to form homogenized emulsions. The emulsified water fuels, prepared using ultrasound, were found to be stable and the size of FWM-CV colonies were effectively reduced to pass through the engine nozzle safely. Engine tests at 3670 rpm were conducted using three fuels: cottonseed biodiesel CS-B100, emulsified cottonseed biodiesel water fuel, water and emulsifier (CS-E20 and emulsified water containing FWM-CV cells CS-ME20. The results showed that the brake specific fuel consumption (BSFC was increased by about 41% when the engine was fueled with emulsified water fuels compared to CS-B100. The engine power, exhaust gas temperature, NOx and CO2 were significantly lower than that produced by CS-B100. The CS-ME20 produced higher power than CS-E20 due to the heating value improvement as a result of adding FWM-CV cells to the fuel.

  7. Analisis Pengaruh Kemampuan, Usaha dan Dukungan Perusahaan terhadap Kinerja Karyawan pada CV Sandang Gloria Konveksindo

    OpenAIRE

    Logahan, Jerry; Sari, Synthia Atas; Marisa, Dian

    2012-01-01

    Clothing Konveksindo CV Gloria is a trading company engaged in the sale of products and clothing items. Therefore, the performance of employees is very important in achieving that goal. The purpose of this study was to analyze the influence of ability, effort, and support the company towards the employee's performance CV Gloria Konveksindo Clothing. The method of analysis used in this study is descriptive, Pearson Regression, and Multiple Regression. Data obtained from the appraisal of employ...

  8. Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.

    Science.gov (United States)

    Garigliany, Mutien-Marie; Hagen, Ralf Matthias; Frickmann, Hagen; May, Jürgen; Schwarz, Norbert Georg; Perse, Amanda; Jöst, Hanna; Börstler, Jessica; Shahhosseini, Nariman; Desmecht, Daniel; Mbunkah, Herbert Afegenwi; Daniel, Achukwi Mbunkah; Kingsley, Manchang Tanyi; Campos, Renata de Mendonca; de Paula, Vanessa Salete; Randriamampionona, Njary; Poppert, Sven; Tannich, Egbert; Rakotozandrindrainy, Raphael; Cadar, Daniel; Schmidt-Chanasit, Jonas

    2014-12-18

    Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution.

  9. Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV)

    International Nuclear Information System (INIS)

    Shi Min; Chen Yafeng; Huang Fang; Liu Pengcheng; Zhou Xueping; Chen Xuexin

    2008-01-01

    Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with IκB-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy

  10. Evaluation of faba beans ( Vicia faba cv. Fiord) and sweet lupins ...

    African Journals Online (AJOL)

    Evaluation of faba beans ( Vicia faba cv. Fiord) and sweet lupins ( Lupizus albus cv. Kiev) as protein sources for ... P igsi n the metabolisrna ndN balance trial consurned5 % less( P < 0.01)o 1'thel upind ietc omparedto the fabab eand iet (l 370 vs. | 440 gld).N o significandt ift'erenceisn the digestiblee nergy( DE) contenta nd ...

  11. Pengaruh Kualitas Pelayanan terhadap Loyalitas Pelanggan di Rental Mobil CV. Ilham Motor Gandu Mlarak Ponorogo

    OpenAIRE

    Dhika Amalia

    2015-01-01

    The purpose of this study was to determine the amount of quality customer service and loyalty to know a very dominant factor affecting customer loyalty in the CV . Ilham Motor. This research method is the primary data from the questionnaires , sampling technique is a technique associated with customer 100 CV Ilham Motor . Data analysis is a quantitative method to test the validity , reliability testing , multiple linear regression analysis , the correlation coefficient analytic , analytic det...

  12. [Habitat factor analysis for Torreya grandis cv. Merrillii based on spatial information technology].

    Science.gov (United States)

    Wang, Xiao-ming; Wang, Ke; Ao, Wei-jiu; Deng, Jin-song; Han, Ning; Zhu, Xiao-yun

    2008-11-01

    Torreya grandis cv. Merrillii, a tertiary survival plant, is a rare tree species of significant economic value and expands rapidly in China. Its special habitat factor analysis has the potential value to provide guide information for its planting, management, and sustainable development, because the suitable growth conditions for this tree species are special and strict. In this paper, the special habitat factors for T. grandis cv. Merrillii in its core region, i.e., in seven villages of Zhuji City, Zhejiang Province were analyzed with Principal Component Analysis (PCA) and a series of data, such as IKONOS image, Digital Elevation Model (DEM), and field survey data supported by the spatial information technology. The results showed that T. grandis cv. Merrillii exhibited high selectivity of environmental factors such as elevation, slope, and aspect. 96.22% of T. grandis cv. Merrillii trees were located at the elevation from 300 to 600 m, 97.52% of them were found to present on the areas whose slope was less than 300, and 74.43% of them distributed on sunny and half-sunny slopes. The results of PCA analysis indicated that the main environmental factors affecting the habitat of T. grandis cv. Merrillii were moisture, heat, and soil nutrients, and moisture might be one of the most important ecological factors for T. grandis cv. Merrillii due to the unique biological and ecological characteristics of the tree species.

  13. Thermal hydraulic research on next generation PWRs using ROSA/LSTF

    International Nuclear Information System (INIS)

    Yonomoto, T.; Anoda, Y.

    2000-01-01

    A thermal-hydraulic research on next generation PWRs has been conducted at JAERI using the ROSA-V/Large Scale Test Facility (LSTF), focusing on phenomena related to passive safety systems. This paper describes two test results conducted for this research: a small break loss-of-coolant accident (SBLOCA) test and a low pressure steady-state natural circulation (NC) test. The former test investigated a combined use of a SG secondary-side automatic depressurization system (SADS) and a gravity-driven injection system (GDIS) to mitigate a SBLOCA. The results have shown that the primary loop can be depressurized to the GDIS actuation pressure of 0.2 MPa by the SADS alone, and then the stable long-term core cooling can be established by NC. Results of both tests showed a complicated nonuniform flow behavior among SG U-tubes during NC, which was characterized by the coexistence of concurrent condensing two-phase flow in some tubes and stagnant two-phase stratification in the others. The mechanism for the stratification was understood from the measured secondary side temperature distribution showing the lowest temperature at the top and bottom regions and the highest around the midplane. This was caused by the saturation temperature difference corresponding to the static pressure difference, and the recirculation in the secondary. This secondary side temperature distribution enabled the condensation occurring around the tube top to be balanced with evaporation occurring around the midplane in the U-tube with the stratification. Since the heat transfer occurs primarily through tubes with the concurrent flow, the nonuniform behavior directly affects the effective heat transfer area at SG. When the SG primary side was modeled with one lumped flow channel, the RELAP5 significantly over predicted the primary depressurization rate, and could not predict the stable long-term core cooling behavior at low pressure. In order to understand the mechanism of the nonuniform behavior, the

  14. Compact and high-sensitivity 100-Gb/s (4 × 25 Gb/s) APD-ROSA with a LAN-WDM PLC demultiplexer.

    Science.gov (United States)

    Yoshimatsu, Toshihide; Nada, Masahiro; Oguma, Manabu; Yokoyama, Haruki; Ohno, Tetsuichiro; Doi, Yoshiyuki; Ogawa, Ikuo; Takahashi, Hiroshi; Yoshida, Eiji

    2012-12-10

    We demonstrate an integrated 100 GbE receiver optical sub-assembly (ROSA) that incorporates a monolithic four-channel avalanche photodiode (APD) array and a planer lightwave circuit (PLC) based LAN-WDM demultiplexer. A record minimum receiver sensitivity of -20 dBm and 50-km error-free SMF transmission without an optical amplifier have been achieved.

  15. The distribution of a non-native (Rosa multiflora) and native (Kalmia latifolia) shrub in mature closed-canopy forests across soil fertility gradients

    Science.gov (United States)

    Cynthia D. Huebner; Jim Steinman; Todd F. Hutchinson; Todd E. Ristau; Alejandro A. Royo

    2014-01-01

    Background and aims. A soil fertility gradient, ranging from infertile to highly fertile soils, may define whether or not a plant will establish and spread at a site. We evaluated whether or not such a fertility gradient exists for Rosa multiflora Thunb., a nonnative invasive shrub, and Kalmia latifolia L., a...

  16. The effects of rose hip (Rosa canina) on plasma antioxidative activity and C-reactive protein in patients with rheumatoid arthritis and normal controls

    DEFF Research Database (Denmark)

    Nielsen, Bente Kirkeskov; Christensen, Robin; Bügel, Susanne Gjedsted

    2011-01-01

    OBJECTIVES: Rose hip (Rosa canina) has been used as an herbal remedy against a wide range of ailments including inflammatory disorders. The anti-inflammatory and antioxidant properties of rose hips have been evaluated in vitro and active constituents have been isolated. Rose hip contains antioxid...

  17. Assessment of risk factors for musculoskeletal disorders using the Rapid Office Strain Assessment (ROSA Method and implementing ergonomics intervention programs in Sepah Bank

    Directory of Open Access Journals (Sweden)

    I. Nasiri

    2015-07-01

    .Conclusion: Using the ROSA technique was seemed to be beneficialto assess the ergonomic risk factors of office works, and the deficiencies in the workstation can be identified through this method. Moreover,by design and implementation of an educational intervention program along with engineering interventions which comply with the elements of this technique, the defects can be eliminated.

  18. Detailed mapping and rupture implications of the 1 km releasing bend in the Rodgers Creek Fault at Santa Rosa, northern California

    Science.gov (United States)

    Hecker, Suzanne; Langenheim, Victoria; Williams, Robert; Hitchcock, Christopher S.; DeLong, Stephen B.

    2016-01-01

    Airborne light detection and ranging (lidar) topography reveals for the first time the trace of the Rodgers Creek fault (RCF) through the center of Santa Rosa, the largest city in the northern San Francisco Bay area. Vertical deformation of the Santa Rosa Creek floodplain expresses a composite pull‐apart basin beneath the urban cover that is part of a broader 1‐km‐wide right‐releasing bend in the fault. High‐resolution geophysical data illuminate subsurface conditions that may be responsible for the complex pattern of surface faulting, as well as for the distribution of seismicity and possibly for creep behavior. We identify a dense, magnetic basement body bounded by the RCF beneath Santa Rosa that we interpret as a strong asperity, likely part of a larger locked patch of the fault to the south. A local increase in frictional resistance associated with the basement body appears to explain (1) distributed fault‐normal extension above where the RCF intersects the body; (2) earthquake activity around the northern end of the body, notably the 1969 ML 5.6 and 5.7 events and aftershocks; and (3) creep rates on the RCF that are higher to the north of Santa Rosa than to the south. There is a significant probability of a major earthquake on the RCF in the coming decades, and earthquakes associated with the proposed asperity have the potential to release seismic energy into the Cotati basin beneath Santa Rosa, already known from damaging historical earthquakes to produce amplified ground shaking.

  19. CARACTERIZAÇÃO FÍSICA, QUÍMICA E NUTRICIONAL DA PITAIA-ROSA DE POLPA VERMELHA

    Directory of Open Access Journals (Sweden)

    MARIA HELENA MENEZES CORDEIRO

    2015-03-01

    Full Text Available RESUMO A pitaia-rosa de polpa vermelha é uma cactácea com potencial para exploração econômica, devido aos seus componentes nutricionais e ao alto valor comercial; no entanto, poucas são as pesquisas quanto a suas características pós-colheita. O objetivo do trabalho foi caracterizar física, química e nutricionalmente pitaias-rosas de polpa vermelha. Frutos colhidos totalmente maduros foram sanitizados, selecionados e divididos em grupos de quatro frutos por repetição, constituindo um total de 30 amostras. Em seguida, as amostras foram submetidas às seguintes avaliações: comprimento, diâmetro, espessura de casca, massa fresca, massa de casca e polpa, teor de umidade, firmeza da casca, sólidos solúveis, pH, acidez titulável, açúcares e fibra bruta, cromaticidade, ângulo hue e luminosidade da casca e da polpa, e o teor de nutrientes. Para os dados obtidos, foram determinados a média, o valor mínimo e máximo, desvio-padrão e o coeficiente de variação. A fruta apresentou valores elevados de conteúdo de água, teor de fibras e dos nutrientes nitrogênio, potássio, cálcio, ferro, manganês e zinco. Quanto ao teor de sólidos solúveis, a média observada foi de 13,14° Brix, e para acidez titulável,0,29 mg de ác. málico 100-1 mL de suco. Verificaram-se para os açúcares valores de 5,56; 8,79 e 3,07 %, respectivamente, para os açúcares redutores, totais e não redutores. Quanto à coloração, os frutos apresentaram valores maiores para luminosidade e cromaticidade da casca e ângulo Hue da polpa. A pitaia-rosa de polpa vermelha (Hylocereus polyrhizus apresenta características físicas, químicas e nutricionais interessantes para o consumo in natura.

  20. [Regularity of Clinical Application of Lianquan (CV 23) in Chinese Ancient Times According to Literature of Traditional Chinese Medicine].

    Science.gov (United States)

    Deng, Mei-Jun; Liu, Chun-Yan; Xie, Yu; Zhu, Jie-Bin; Xu, Zhen-Hua

    2018-03-25

    To summarize the regularity of application of Lianquan (CV 23) in clinical practice in Chinese ancient times through analysis of ancient traditional Chinese medical (TCM) literature. A total of 60 books involving CV 23 from the 1 156 ancient TCM books listed in the fifth edition of Encyclopedia of Traditional Chinese Medicine were collected by using CV 23 as the main keyword and "Sheben" "Benchi", and "Jieben" (the other names of CV 23 in TCM)as the supplementary keywords and analyzed systematically. A database was then constructed from the collected data, including the related types of disorders or symptoms, acupoint recipes, and methods of needling and moxibustion, contraindications, etc. A total of 196 articles related to the application of CV 23 from 60 ancient classical books were collected in accordance with the inclusive criteria. Among them, 155 articles are referred to the indications of CV 23, 35 to types of disorders such as asthma, cough, tongue swelling with difficulty in speaking, protracted tongue, acute contraction of tongue root, vomiting, spasm syndrome, stroke, aphtha, problems of mouth and teeth, throat problems, etc. of the internal medicine, surgery, pediatrics, and five-sense organs; 64 items are referred to the application of single CV 23, 91 to CV 23-included recipes containing 111 adjunct acupoints, and 78 to stimulation of CV 23 with acupuncture needle, moxibustion, pricking blood, and fire needle. Moreover, of the 111 adjunct acupoints, the most commonly used are Shaoshang (LU 11), Tiantu (CV 22), Hegu (LI 4), Yuye (EX-HN 13), Zhongchong (PC 9), etc. Lianquan (CV 23) is mainly used for glossopharyngeal problems chiefly by syndrome-meridian differentiation. The supplement of complementary acupoints or five-shu points in combination with CV 23 has a synergistic effect. Moxibustion (3 moxa- cones in general) is often employed, and the needling depth is usually about 7.5 mm. The common contraindication of CV 23 is severe tongue swelling.

  1. Diversidad de coleópteros en un bosque alto andino del municipio de Santa Rosa de Viterbo (Boyacá (Diversity of coleoptera in a high forest Andean in the municipality of Santa Rosa de Viterbo (Boyacá

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    Humberto Bohórquez Salazar

    2016-09-01

    Full Text Available Este artículo presenta los resultados de un estudio desarrollado en el municipio de Santa Rosa de Viterbo, ubicado a 2750 msnm, en un relicto de bosque húmedo montano bajo (bh-M. Dicho estudio tuvo como finalidad identificar la diversidad y riqueza de entomofauna referida al orden Coleóptera, en los predios de la Escuela de Policía Rafael Reyes (ESREY. Es importante señalar que este es el primer estudio de este tipo realizado en la zona. Se reportan un total de 270 individuos capturados pertenecientes a 17 familias, 25 subfamilias, 30 tribus y 44 géneros; siendo Coccinellidae la familia más representativa (19,3% y Dryophthoridae la familia menos representativa (0,4%. Vale la pena mencionar que, según lo reportado por Martínez (2005, no se encuentran registros de ningún género de Carabidae en el municipio, por lo que se puede destacar que los resultados publicados en este artículo son los primeros registros para Santa Rosa de Viterbo. Así mismo, la revisión de Medina, Lopera-Toro, Vitolo & Gill, (2001 sobre escarabajos coprófagos de Colombia indica que los géneros encontrados en este estudio no se registran a una altitud mayor a 2600 msnm por lo que se puede reportar un registro altitudinal para Canthon y Dichotomius Finalmente, en el artículo se presentan los resultados de un proceso de educación desarrollado con niños de grado quinto de primaria de una institución educativa del municipio y con los semilleros de investigación de la ESREY, proceso que fue diseñado como estrategia para promover la apropiación social de conocimiento y divulgación de los resultados del trabajo. (Abstract. This article presents the results of a study conducted in the municipality of Santa Rosa de Viterbo, located at 2750 meters above sea level, in a relict humid montane forest under (bh-M. This study aimed to identify the diversity and richness of Coleoptera order entomofauna referred to in the grounds of the School of Police Rafael Reyes

  2. Conservação de tangerina cv. Clemenules utilizando diferentes recobrimentos = Conservation of Mandarins cv. Clemenules with different cuticles

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    Rosa de Oliveira Treptow

    2007-01-01

    Full Text Available O objetivo deste trabalho foi verificar os efeitos de diferentes recobrimentos e períodos de armazenamento na conservação de tangerinas Clemenules. Após a colheita, as frutas foram submetidas ao pré-resfriamento por 12 horas a 5ºC e em seguida, aos seguintes tratamentos: T1 testemunha; T2 filme de polietileno microperfurado; T3 filme de polietileno não perfurado; T4 cera de carnaúba a 50%, diluída em água; T5 cera de carnaúba a 100% (não diluída. As tangerinas foram armazenadas por 20, 40 e 60 dias em temperatura de 5ºC e UR de 90-95%, depois foram mantidas em temperatura de 15±1ºC e UR de 75-80%, durante quatro dias simulando a comercialização. Na colheita e após cada período de armazenamento, seguido de simulação decomercialização, foram avaliadas as variáveis: perda de massa; cor; sólidos solúveis (SS; acidez titulável (AT; relação SS/AT; podridões, distúrbios fisiológicos e características sensoriais. Amodificação da atmosfera reduziu a perda de massa, a incidência de distúrbios e podridões de tangerinas Clemenules durante o armazenamento. As frutas acondicionadas em filme de polietilenosem perfuração ou aquelas revestidas com cera sem diluição apresentaram sabor estranho e menor qualidade geral. Tangerinas ‘Clemenules’ acondicionadas em filme de polietileno microperfurado ecera diluída em 50% de água podem ser armazenadas durante 60 dias a 5ºC e comercializadas durante quatro dias a 15±1ºC sem comprometer a qualidade sensorial.The aim of this work was to evaluate the effects of different cuticles and storage periods on the conservation of mandarins cv. Clemenules. After harvest the fruits were kept at 5ºC during 12 hours. Then, they were submitted to the following treatments: T1 control; T2 micro perforated polyethylene bag; T3 non-perforated polyethylene bag; T4 carnauba wax at 50% in water; T5 carnauba wax at 100% (without dilution. The mandarins were stored for 20, 40 and 60 days at 5

  3. Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril Productivity and nutritional value of Brachiaria brizantha cv. Marandu in a silvopastoral system

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    L.F. Sousa

    2007-08-01

    Full Text Available O experimento foi realizado em sistema silvipastoril (Ssp localizado no bioma de Cerrado, no município de Lagoa Santa, Minas Gerais, no ano agrícola de 2004-2005. Avaliou-se a influência do Ssp composto pela arbórea bolsa-de-pastor (Zeyheria tuberculosa sobre os parâmetros produção, atributos morfogênicos, composição bromatológica e valor nutritivo da gramínea Brachiaria brizantha cv. Marandu, e os efeitos sobre as condições microclimáticas. O delineamento experimental foi em blocos ao acaso, com cinco blocos (épocas de corte, dois tratamentos (T1 - B. brizantha cv. Marandu sombreada com a Z. tuberculosa e T2 - B. brizantha cv. Marandu, a pleno sol e três repetições (parcelas. O sombreamento proporcionado pela Z. tuberculosa, apesar de reduzir a produção de matéria seca (MS da gramínea, favoreceu o aumento do teor de proteina bruta (PB e não alterou a produção de PB/ha e nem afetou a concentração de FDN. Os teores de FDA foram mais altos que os encontrados a pleno sol, o que provavelmente resultou em menor degradabilidade in vitro da MS e da matéria orgânica, não alterando a degradabilidade efetiva.The experiment was carried out in a Silvopastoral system (Sps located in Brazilian Savannah, Lagoa Santa county, Minas Gerais State, during the agricultural year of 2004-2005. It was studied the influence of Sps formed by "Bolsa de Pastor" (Zeyheria tuberculosa on the production, morphogenic attributes, bromatological composition and nutritional value of the graminaceous Brachiaria brizantha cv. Marandu as well as on the microclimatic aspects. The Sps was established in 1982, in a red-yellow latossol by the natural regeneration process. The experiment was based on a randomized block design, with 5 blocks (cutting times, 2 treatments (T1 - B. brizantha cv. Marandu shaded by Z. tuberculosa and T2 - B. brizantha cv. Marandu at open sky and three repetitions (plots. Although the DM production of the graminaceous in the Sps

  4. FORMULASI STRATEGI PENINGKATAN PRODUKSI DOMBA CV MITRA TANI FARM, CIAMPEA, BOGOR

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    Amalia Sholehana

    2014-04-01

    Full Text Available ABSTRACTThis research was conducted to formulate to increase the sheep production at CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. The research was conducted using descriptive analysis by analyzing the problem experienced by CV. MitraTani Farm. Primary data and secondary data were gathered at this research. The research was also conducted using several analytical methods such as value chain mapping, internal factor evaluation (IFE and external factor evaluation (EFE matrix, SWOT and quantitative strategic planning matrix (QSPM. Based on the value chain analysis, the current production of sheep at CV MT farm is highly depending on the supply from its partners and internal farmers. According to the IFE and EFE results, the differences of each weighted values were respectively 2,120 and 0,686 so the position of the company in the SWOT matrix was situated at Quadrant I. Therefore the company needs to use a growth strategy. The QSPM sequenced the strategy priority as follow (1 increasing the number of lambs, (2 developing the management, (3 strengthening the capital and ownership, (4 improving the quality through technology. The result of the gross profit margin calculation, if the sheep‘s selling is increased up to 1000 sheep per month then the possible annual profit that can be earned by CV MT farm is 40,34% of the total production.Keywords: MT Farm, sheep production, strategies, SWOT, QSPM, gross profit marginABSTRAKPenelitian ini dilakukan untuk memformulasikan strategi peningkatan produksi domba di CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. Pendekatan penelitian yang diterapkan adalah analisis deskriptif yang dilakukan dengan mempelajari permasalahan dari objek yang diteliti. Data yang dikumpulkan dalam penelitian ini adalah data primer dan data sekunder. Penelitian dilakukan menggunakan alat analisis berupa pemetaan rantai nilai,  matriks IFE (Internal Factor Evaluation dan EFE (External Factor Evaluation, SWOT, dan QSPM (Quantitative

  5. Phytophagous Arthropod Species Associated with Oil Bearing Rose, Rosa damascena Miller, in Isparta Province with Distributional Remarks

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    Ozan Demirözer

    2011-05-01

    Full Text Available Abstract: The study is based on field researches made in 2006–2007 in order to determine the phytophagous arthropod species and the deployment of those species that are economically important in the oil-bearing rose production fields in the province of Isparta. As a result, 62 species were determined, 60 of which belongs to 24 families of 6 orders in Insecta and 2 of which belongs to 2 families of 1 order in Arachnida. In the study, 57 species were identified up to species level and 5 species were identified up to genus level. It is seen that 20 species of them are previously identified as pests in Rosa damascena Miller in production fields in the literature. Ten of the species determined in the study are recorded as new for the oil-bearing rose pest fauna in the province of Isparta. Key words: Oil Bearing Rose, Rosa damascena, pest, phytophagous, Isparta Özet: Bu çalışma, Isparta ili yağ gülü üretim alanlarında bulunan fitofag türlerin belirlenmesi ve ekonomik açıdan önemlilerinin yayılışlarının ortaya çıkarılması amacıyla 2006-2007 yılları arasında yürütülmüştür. Çalışma sonucunda, Insecta sınıfının 6 takımına ait 24 familyadan toplam 60, Arachnida sınıfına ait 1 takıma bağlı 2 familyadan 2 olmak üzere toplamda 62 türün varlığı belirlenmiştir. Çalışmada 57 adet örneğin tür düzeyinde teşhisleri tamamlanırken, 5 türün cins düzeyine kadar teşhisleri yapılmıştır. Yapılan literatür çalışmalarında bu araştırmada belirlenen türlerden 20'sinin daha önceden Rosa damascena Miller üretim alanlarında zararlı olarak bildirildiği saptanmıştır. Çalışmada belirlenen türlerden 10'u ise Isparta ili yağ gülü zararlı faunası için yeni kayıt niteliği taşımaktadır. Anahtar kelimeler: Yağ gülü, Rosa damascena, zararlı, fitofag, Isparta

  6. Heat stability of strawberry anthocyanins in model solutions containing natural copigments extracted from rose (Rosa damascena Mill.) petals.

    Science.gov (United States)

    Shikov, Vasil; Kammerer, Dietmar R; Mihalev, Kiril; Mollov, Plamen; Carle, Reinhold

    2008-09-24

    Thermal degradation and color changes of purified strawberry anthocyanins in model solutions were studied upon heating at 85 degrees C by HPLC-DAD analyses and CIELCh measurements, respectively. The anthocyanin half-life values increased significantly due to the addition of rose (Rosa damascena Mill.) petal extracts enriched in natural copigments. Correspondingly, the color stability increased as the total color difference values were smaller for anthocyanins upon copigment addition, especially after extended heating. Furthermore, the stabilizing effect of rose petal polyphenols was compared with that of well-known copigments such as isolated kaempferol, quercetin, and sinapic acid. The purified rose petal extract was found to be a most effective anthocyanin-stabilizing agent at a molar pigment/copigment ratio of 1:2. The results obtained demonstrate that the addition of rose petal polyphenols slows the thermal degradation of strawberry anthocyanins, thus resulting in improved color retention without affecting the gustatory quality of the product.

  7. Characterization of biochemical traits of dog rose (Rosa canina L.) ecotypes in the central part of Iran.

    Science.gov (United States)

    Javanmard, Milad; Asadi-Gharneh, Hossein Ali; Nikneshan, Pejman

    2018-07-01

    Dog rose (Rosa canina L.) is a wild native species in Iran, with a significant genetic diversity. This plant serves as a rich source of vitamin C, anthocyanins, phenolic contents and carotenoids. Rose hips have been used in several food products, as well as perfumery and cosmetics industries. In this research, we investigate biochemical characteristics of five dog rose ecotypes (Kopehjamshid, Zarneh, Miyankish, Aghcheh and Sadeghiyeh), that were collected from the central part of Iran (Isfahan province). Amounts of vitamin C, total carotenoids, total phenolic contents, total anthocyanins, macro and micro minerals were measured. Seed oil are extracted by soxhlet method and analysed by gas chromatography. The macro and micro minerals levels in the fruit vary significantly among these regions. The results of this study demonstrate that dog rose have great diversity and can be used in breeding programmes in order to increase nutrient values as a food resource additive.

  8. Dog rose (Rosa canina L.) as a functional ingredient in porcine frankfurters without added sodium ascorbate and sodium nitrite.

    Science.gov (United States)

    Vossen, Els; Utrera, Mariana; De Smet, Stefaan; Morcuende, David; Estévez, Mario

    2012-12-01

    The effect of dog rose (Rosa canina L.; RC), rich in polyphenols and ascorbic acid, on lipid and protein oxidation, colour stability and texture of frankfurters was investigated. Four treatments were prepared: with 5 or 30 g/kg RC extract and without sodium ascorbate and sodium nitrite (5RC and 30RC, respectively), a positive control (with sodium ascorbate and sodium nitrite; PC) and a negative control (without sodium ascorbate, sodium nitrite or RC extract; NC). Hexanal values were much higher throughout storage in NC compared to RC and PC frankfurters (Prose can act as a natural antioxidant in frankfurters, but not as full replacer for sodium nitrite. Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. Aporia e passagem: a sobrevivência do "trágico" em Guimarães Rosa

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    Ettore Finazzi-Agrò

    2002-03-01

    Full Text Available O intuito deste texto é pôr em questão a persistência do “pensamento trágico” em Grande sertão: veredas, a partir de uma reflexão sobre o estatuto essencialmente (i.e., ontologicamente aporético da obra de João Guimarães Rosa. Romance da espera, de fato, ele se inclui por completo naquele lugar “terceiro”, suspenso entre esperança e desespero, que se delineia no interior de qualquer contradição e que constitui, desde sempre, o logos da tragédia.

  10. Notes on (un-defeated revolution. Remarks in the margin of Rosa Luxemburg’s works from 1905-1906.

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    Kamil Piskała

    2012-01-01

    Full Text Available This article attempts to present some reflections about Rosa Luxemburg’sworks from the first Russian revolution (1905-1906. I consider Luxemburg’sview on the historical meaning of this revolution and discuss her analyses of classstruggle in 1905-1906. The description of class struggle’s forms and its dynamicsis the most important and interesting excerpt of Luxemburg’s works from this time.She emphasized the meaning of a revolutionary sense of freedom and the changingworkers consciousness that happens during the revolution. She presents an inspiringdialectic relation between defeated rebellion or revolution and the final victory ofsocialist movement. I think that Luxemburg’s perspective may be useful for researchon contemporary social struggles (e.g. “Arab Spring”, “Occupy!” and helpful insearching for new forms of organization for radical liberation movements.

  11. Incremento anual do Pau-rosa (Aniba duckei Kostermans) Lauraceae, em floresta tropical úmida primária. ()

    OpenAIRE

    Alencar, Jurandyr da Cruz; Araujo, Vivaldo Campbell de

    1981-01-01

    Resumo Os autores analisam o incremento periódico anual do diâmetro (D.A.P.) de vinte árvores de "Pau-rosa" (Aniba duckei Kostermans) durante o período de 12,5 anos em floresta tropical primária na Reserva Ducke, na Amazônia Central. O incremento periódico anual variou de 0,16 cm a 0.74 cm/ano sendo a média 0.38 cm/ano. O incremento periódico médio, para as árvores tomadas em conjunto, cresce a partir de janeiro, época de chuvas, atinge o máximo em julho e decresce durante a estação seca. Tes...

  12. Re/position? "Tres Marías y una Rosa" three decades to address the resistance

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    Yael Zaliasnik

    2010-11-01

    Full Text Available The present work explores the conditions and repercussions of staging anew an emblematic play of the dictatorship era in Chile: "Tres Marías y una Rosa," three decades after that period, and without significant modifications. In the words of Raymond Williams, what's the residual, the dominant, the emergent? What is won/lost in this re-staging? How is it perceived ("read"? Are there still residual social images from the dictatorship? What did the play represent 30 years ago, and has that changed today? Inevitably, these questions lead us to the issue of the Chilean textile craft of the "arpilleras" and the women artisans who made these ("arpilleristas", to our understanding, central to the play, but avoided and forgotten as a resistance movement, as it is not treated in depth in its current staging.

  13. Fire and vegetation history on Santa Rosa Island, Channel Islands, and long-term environmental change in southern California

    Science.gov (United States)

    Starratt, Scott W.; Pinter, N.; Anderson, Robert S.; Jass, R.B.

    2009-01-01

    The long-term history of vegetation and fire was investigated at two locations – Soledad Pond (275 m; from ca. 12 000 cal. a BP) and Abalone Rocks Marsh (0 m; from ca. 7000 cal. a BP) – on Santa Rosa Island, situated off the coast of southern California. A coastal conifer forest covered highlands of Santa Rosa during the last glacial, but by ca. 11 800 cal. a BP Pinus stands, coastal sage scrub and grassland replaced the forest as the climate warmed. The early Holocene became increasingly drier, particularly after ca. 9150 cal. a BP, as the pond dried frequently, and coastal sage scrub covered the nearby hillslopes. By ca. 6900 cal. a BP grasslands recovered at both sites. Pollen of wetland plants became prominent at Soledad Pond after ca. 4500 cal. a BP, and at Abalone Rocks Marsh after ca. 3465 cal. a BP. Diatoms suggest freshening of the Abalone Rocks Marsh somewhat later, probably by additional runoff from the highlands. Introduction of non-native species by ranchers occurred subsequent to AD 1850. Charcoal influx is high early in the record, but declines during the early Holocene when minimal biomass suggests extended drought. A general increase occurs after ca. 7000 cal. a BP, and especially after ca. 4500 cal. a BP. The Holocene pattern closely resembles population levels constructed from the archaeological record, and suggests a potential influence by humans on the fire regime of the islands, particularly during the late Holocene.

  14. Chemical composition, leishmanicidal and cytotoxic activities of the essential oils from Mangifera indica L. var. Rosa and Espada.

    Science.gov (United States)

    Ramos, Eduardo H S; Moraes, Marcílio M; Nerys, Laís L de A; Nascimento, Silene C; Militão, Gardênia C G; de Figueiredo, Regina C B Q; da Câmara, Cláudio A G; Silva, Teresinha Gonçalves

    2014-01-01

    The essential oils from Mangifera indica var. Rosa and Espada latex were obtained by hydrodistillation and analyzed using GC-FID and GC-MS. Twenty-seven components were identified. The main compound in the essential oil from M. indica var. Espada (EOMiE) was terpinolene (73.6%). The essential oil of M. indica var. Rosa (EOMiR) was characterized by high amounts of β-pinene (40.7%) and terpinolene (28.3%). In the test for leishmanicidal activity against promastigotes forms of L. amazonensis, EOMiR and EOMiE showed IC50 (72 h) of 39.1 and 23.0 μg/mL, respectively. In macrophages, EOMiR and EOMiE showed CC50 of 142.84 and 158.65 μg/mL, respectively. However, both were more specific to the parasite than macrophages, with values of selectivity index of 6.91 for EOMiE and 3.66 for EOMiR. The essential oils were evaluated for their cytotoxicity against the human tumor cells HEp-2, HT-29, NCI-H292, and HL-60. The EOMiR and EOMiE were most effective against the HL-60, with IC50 values of 12.3 and 3.6 μg/mL, respectively. The results demonstrated that the essential oils of M. indica can destroy L. amazonensis and inhibit tumor cell growth. These findings contribute to the knowledge of the Brazilian biodiversity as a source of potential therapeutic agents.

  15. Effects of aromatherapy with Rosa damascena on nulliparous women's pain and anxiety of labor during first stage of labor.

    Science.gov (United States)

    Hamdamian, Sepideh; Nazarpour, Soheila; Simbar, Masoumeh; Hajian, Sepideh; Mojab, Faraz; Talebi, Atefeh

    2018-03-01

    Reducing labor pain and anxiety is one of the most important goals of maternity care. This study aimed to assess the effects of aromatherapy with Rosa damascena on pain and anxiety in the first stage of labor among nulliparous women. This was a randomized clinical trial of 110 nulliparous women. The eligible participants were randomly assigned to two groups of aromatherapy and control in an Iranian maternity hospital. The participants received 0.08 mL of Rosa damascena essence in the aromatherapy group and 0.08 mL of normal saline in the control group, every 30 min. Pain was measured 3 times, once each at three stages of cervical dilation (4-5, 6-7, and 8-10 cm). Anxiety was measured twice, once each at two stages of cervical dilation (4-7 and 8-10 cm). The tools for data collection were the Spielberger anxiety questionnaire, numerical pain rating scale, demographic and obstetric questionnaire, and an observational checklist. Data analyses included the t-test, Mann-Whitney U test and Chi-square test. Severity of labor pain and severity of anxiety were used as primary outcome measures. Labor and delivery characteristics (including number of contractions, duration of contractions in second stage, Bishop score, augmentation by oxytocin, Apgar score, and mode of delivery), demographic characteristics, and fertility information were used as secondary outcome measures. Pain severity in the group receiving aromatherapy with R. damascena was significantly lower than in the control group after treatment at each pain assessment (cervical dilation of 4-5, 6-7, and 8-10 cm; P labor. Aromatherapy with R. damascena is a convenient and effective method for pain and anxiety reduction during the first stage of labor. Iranian Registry of Clinical Trial: IRCT201306258801N3. Copyright © 2018 Shanghai Changhai Hospital. Published by Elsevier B.V. All rights reserved.

  16. 3CV+2: modelo de calidad para la construcción de la vivienda 3CV+2: quality model for dwelling construction

    Directory of Open Access Journals (Sweden)

    Salvador García Rodríguez

    2008-08-01

    Full Text Available Este artículo presenta el modelo y los resultados que, desde Junio de 2005, se han alcanzado en el desarrollo del Programa Nacional de Certificación de los Procesos Constructivos de la Vivienda en base al Modelo 3cv+2. El programa se ha desarrollado con base a un convenio entre el Tecnológico de Monterrey y la Cámara Nacional de la Industria de Desarrollo y Promoción de Vivienda. Actualmente se encuentran voluntariamente en el programa 52 empresas inmobiliarias de diferentes estados de la República Mexicana. El modelo de calidad 3cv+2 es una metodología que busca establecer en los niveles operativos de la construcción criterios que permitan reducir la variabilidad del proceso de construcción, y además permita de manera sistemática, y en un proceso de mejora continua, evaluar el desempeño de calidad en proyectos de construcción de vivienda. El modelo 3cv+2 define en su nombre el alcance del mismo, 3 criterios de calidad para la vivienda (insumos, proceso y producto, apoyado por 2 auditorías (+2, la auditoría interna de la propia empresa, mas la auditoría externa hecha por el Tecnológico de Monterrey. El modelo 3cv+2 busca garantizar que los insumos utilizados en la construcción de las viviendas sean de calidad superior, y que los procesos de ejecución posean características de calidad, productividad, eficiencia, racionalización, y estandarización; de tal manera que insumos y productos sean de alto valor agregado, y garanticen la calidad especificada en las etapas parciales y en la vivienda terminada.This paper presents the model and the results that have been achieved during the development of the National Quality Certification Program based on the model 3cv +2 from June 2005. This program has been developed based on an agreement between the Monterrey Tech and the National Chamber of Housing Industry. Nowadays, the program has 52 voluntary real estate companies from different states of Mexico. The 3cv+2 quality model is a

  17. Studies of frequency dependent C-V characteristics of neutron irradiated p+-n silicon detectors

    International Nuclear Information System (INIS)

    Li, Zheng; Kraner, H.W.

    1990-10-01

    Frequency-dependent capacitance-voltage fluence (C-V) characteristics of neutron irradiated high resistivity silicon p + -n detectors have been observed up to a fluence of 8.0 x 10 12 n/cm 2 . It has been found that frequency dependence of the deviation of the C-V characteristic (from its normal V -1/2 dependence), is strongly dependent on the ratio of the defect density and the effective doping density N t /N' d . As the defect density approaches the effective dopant density, or N t /N' d → 1, the junction capacitance eventually assumes the value of the detector geometry capacitance at high frequencies (f ≤ 10 5 Hz), independent of voltage. A two-trap-level model using the concept of quasi-fermi levels has been developed, which predicts both the effects of C-V frequency dependence and dopant compensation observed in this study

  18. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.

    Science.gov (United States)

    Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

    2015-05-01

    Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater.

  19. Light equation in eclipsing binary CV Boo: third body candidate in elliptical orbit

    Science.gov (United States)

    Bogomazov, A. I.; Kozyreva, V. S.; Satovskii, B. L.; Krushevska, V. N.; Kuznyetsova, Y. G.; Ehgamberdiev, S. A.; Karimov, R. G.; Khalikova, A. V.; Ibrahimov, M. A.; Irsmambetova, T. R.; Tutukov, A. V.

    2016-12-01

    A short period eclipsing binary star CV Boo is tested for the possible existence of additional bodies in the system with a help of the light equation method. We use data on the moments of minima from the literature as well as from our observations during 2014 May-July. A variation of the CV Boo's orbital period is found with a period of {≈}75 d. This variation can be explained by the influence of a third star with a mass of {≈}0.4 M_{⊙} in an eccentric orbit with e≈0.9. A possibility that the orbital period changes on long time scales is discussed. The suggested tertiary companion is near the chaotic zone around the central binary, so CV Boo represents an interesting example to test its dynamical evolution. A list of 14 minima moments of the binary obtained from our observations is presented.

  20. Segmentation of kidney using C-V model and anatomy priors

    Science.gov (United States)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.