WorldWideScience

Sample records for riverbank protection bioengineering

  1. Bioengineering Techniques for Soil Erosion Protection and Slope Stabilization

    OpenAIRE

    Julia Georgi; Ioannis Stathakopoulos

    2006-01-01

    The use of bio-engineering methods for soil erosion protection and slope stabilization has a long tradition. Old methods with rocks and plants, structures of timber have been used over the past centuries. Recently these old soil conservation and stabilization techniques have been rediscovered and improved. Biotechnical engineering methods have become part of geotechnical and hydraulic engineering and have helped bridge the gap between classical engineering disciplines, land use management, la...

  2. History of bioengineering techniques for erosion control in rivers in Western Europe.

    Science.gov (United States)

    Evette, Andre; Labonne, Sophie; Rey, Freddy; Liebault, Frederic; Jancke, Oliver; Girel, Jacky

    2009-06-01

    Living plants have been used for a very long time throughout the world in structures against soil erosion, as traces have been found dating back to the first century BC. Widely practiced in Western Europe during the eighteenth and nineteenth centuries, bioengineering was somewhat abandoned in the middle of the twentieth century, before seeing a resurgence in recent times. Based on an extensive bibliography, this article examines the different forms of bioengineering techniques used in the past to manage rivers and riverbanks, mainly in Europe. We compare techniques using living material according to their strength of protection against erosion. Many techniques are described, both singly and in combination, ranging from tree planting or sowing seeds on riverbanks to dams made of fascine or wattle fences. The recent appearance of new materials has led to the development of new techniques, associated with an evolution in the perception of riverbanks.

  3. Suitability of soil bioengineering techniques in Central America: a case study in Nicaragua

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2008-10-01

    In the last few years "D. I. A. F." (Department of Agriculture and Forestry Engineering of Florence University), has been testing the effectiveness of soil bioengineering techniques in Central America. The focus of the present study was to find out which native plants were most suited for soil bioengineering purposes, particularly in the realization of riverbank protection in Nicaragua. Furthermore, we have also been aiming at economic efficiency. These techniques are appropriate for sustainable watershed management especially in underdeveloped countries. Concerning the plants to be used we experimented four native species. Gliricidia Sepium, Cordia dentata and Jatropha curcas are suitable for soil bioengineering more than Bursera Simaruba. Economically speaking, the sustainability of such interventions in underdeveloped countries, has been shown by the evaluation of the cost of riverbank protection using vegetated crib-walls in Nicaragua compared to the cost in different contexts.

  4. Soil bio-engineering for risk mitigation and environmental restoration in a humid tropical area

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2009-07-01

    The use of soil bio-engineering techniques in developing countries is a relevant issue for disaster mitigation, environmental restoration and poverty reduction. Research on authochtonal plants suitable for this kind of works and on economic efficiency is essential for the divulgation of such techniques. The present paper is focused on this two issues related to the realization of various typologies of soil bio-engineering works in the humid tropic of Nicaragua. In the area of Río Blanco, located in the Department of Matagalpa, soil bio-engineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in the works, monitorings were performed, one in the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, collecting survival rate and morphological parameters data. Concerning the economic efficiency we proceed to a financial analysis of the works and once the unit price was obtained, we converted the amount in EPP Dollars (Equal Purchasing Power) in order to compare the Nicaraguan context with the Italian one. Among the used species we found that Madero negro (Gliricidia sepium) and Roble macuelizo (Tabebuia rosea) are adequate for soil-bioengineering measure on slopes while Helequeme (Erythrina fusca) reported a successful behaviour only in the crib wall for riverbank protection. In the comparison of the costs in Nicaragua and in Italy, the unit price reduction for the Central American country ranges between 1.5 times (for the vegetative covering) and almost 4 times (for the fascine mattress) if it's used the EPP dollar exchange rate. Conclusions are reached with regard to hydrological-risk mitigating actions performed on a

  5. Soil bioengineering for risk mitigation and environmental restoration in a humid tropical area

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2010-02-01

    The use of soil bio-engineering techniques in developing countries is a relevant issue for disaster mitigation, environmental restoration and poverty reduction. Research on the autochthonal plants suitable for these kinds of interventions and on the economic efficiency of the interventions is essential for the dissemination of such techniques. The present paper is focused on these two issues as related to the realization of various typologies of soil bioengineering works in the humid tropics of Nicaragua. In the area of Río Blanco, located in the Department of Matagalpa, soil bioengineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in these works, monitoring was performed, one on the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, by collecting data on survival rate and morphological parameters. Concerning economic efficiency, we proceeded to a financial analysis of the works. Once the unit price was obtained, we converted the amount into EPP Dollars (Equal Purchasing Power) in order to compare the Nicaraguan context with the European one. Among the species used we found that Gliricidia sepium (local common name: Madero negro) and Tabebuia rosea (local common name: Roble macuelizo) are adequate for soil bioengineering measures on slopes, while Erythrina fusca (local common name: Helequeme) resulted in successful behaviour only in the crib wall for riverbank protection. In comparing costs in Nicaragua and in Italy, the unit price reduction for Nicaragua ranges from 1.5 times (for the vegetative covering) to almost 4 times (for the fascine mattress), using the EPP dollar exchange rate. Our conclusions with

  6. Soil bioengineering for risk mitigation and environmental restoration in a humid tropical area

    Directory of Open Access Journals (Sweden)

    A. Petrone

    2010-02-01

    Full Text Available The use of soil bio-engineering techniques in developing countries is a relevant issue for disaster mitigation, environmental restoration and poverty reduction. Research on the autochthonal plants suitable for these kinds of interventions and on the economic efficiency of the interventions is essential for the dissemination of such techniques. The present paper is focused on these two issues as related to the realization of various typologies of soil bioengineering works in the humid tropics of Nicaragua.

    In the area of Río Blanco, located in the Department of Matagalpa, soil bioengineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in these works, monitoring was performed, one on the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, by collecting data on survival rate and morphological parameters. Concerning economic efficiency, we proceeded to a financial analysis of the works. Once the unit price was obtained, we converted the amount into EPP Dollars (Equal Purchasing Power in order to compare the Nicaraguan context with the European one.

    Among the species used we found that Gliricidia sepium (local common name: Madero negro and Tabebuia rosea (local common name: Roble macuelizo are adequate for soil bioengineering measures on slopes, while Erythrina fusca (local common name: Helequeme resulted in successful behaviour only in the crib wall for riverbank protection.

    In comparing costs in Nicaragua and in Italy, the unit price reduction for Nicaragua ranges from 1.5 times (for the vegetative covering to almost 4 times (for the fascine mattress

  7. Bioengineered nanomaterials

    CERN Document Server

    Tiwari, Atul

    2013-01-01

    Many varieties of new, complex diseases are constantly being discovered, which leaves scientists with little choice but to embrace innovative methods for controlling the invasion of life-threatening problems. The use of nanotechnology has given scientists an opportunity to create nanomaterials that could help medical professionals in diagnosing and treating problems quickly and effectively. Bioengineered Nanomaterials presents in-depth information on bioengineered nanomaterials currently being developed in leading research laboratories around the world. In particular, the book focuses on nanom

  8. Soil bioengineering measures for disaster mitigation and environmental restoration in Central America: authochtonal cuttings suitability and economic efficiency

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2009-04-01

    The use of Soil Bio-Engineering techniques in Developing countries is a relevant issue for Disaster mitigation, environmental restoration and poverty reduction. Research on authochtonal plants suitable for this kind of works and on economic efficiency is essential for the divulgation of this Discipline. The present paper is focused on this two issues related to the realization of various typologies of Soil Bio-engineering works in the Humid tropic of Nicaragua. In the area of Río Blanco, located in the Department of Matagalpa, Soil bio-engineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in the works, monitorings were performed, one in the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, collecting survival rate and morphological parameters data. Concerning the economic efficiency we proceed to a financial analysis of the works and once the unit price was obtained, we converted the amount in EPP Dollars (Equal Purchasing Power) in order to compare the Nicaraguan context with the Italian one. Among the used species we found that Madero negro (Gliricidia sepium) and Roble macuelizo (Tabebuia rosea) are adequate for Soil-bioengineering measure on slopes while Helequeme (Erythrina fusca) reported a successful behaviour only in the crib wall for riverbank protection. In the comparison of the costs in Nicaragua and in Italy, the unit price reduction for the central American country ranges between 1.5 times (for the vegetative covering) and almost 4 times (for the fascine mattress) if it's used the EPP dollar exchange rate. Thus, a conclusion can be reached with regard to hydrological-risk mitigating actions

  9. Soil Bioengineering Application and Practices in Nepal

    Science.gov (United States)

    Dhital, Yam Prasad; Kayastha, Rijan Bhakta; Shi, Jiancheng

    2013-02-01

    The small mountainous country Nepal is situated in the central part of the Himalayas. Its climate varies from tropical in the south to arctic in the north; and natural vegetation follows the pattern of climate and altitude. Water-induced disaster problems including soil erosion, debris flow, landslides and flooding are common due to the unstable landscape. Soil erosion is the most important driving force for the degradation of upland and mountain ecosystems. Soil bioengineering has been used in Nepal for nearly 30 years to deal with erosion problems on slopes, in high way construction and riverbank stabilization. The main soil bioengineering techniques used in Nepal are brush layering, palisades, live check dams, fascines and vegetative stone pitching. This study is based on the geology, climate and vegetation of Nepal and briefly summarizes the application of soil bioengineering on slopes and stream banks, with especial attention to the role of vegetation on slope and stream bank stabilization. Furthermore, this paper addresses the role of community participation and responsibility for successful application of vegetation-based techniques in management, maintenance and utility aspects for the future. In recent years, soil bioengineering techniques are extensively used due to their cost-effectiveness, using locally available materials and low-cost labour in comparison to more elaborate civil engineering works. However, scientific implementation and record-keeping and evaluation of the work are indeed essential.

  10. Soil bioengineering application and practices in Nepal.

    Science.gov (United States)

    Dhital, Yam Prasad; Kayastha, Rijan Bhakta; Shi, Jiancheng

    2013-02-01

    The small mountainous country Nepal is situated in the central part of the Himalayas. Its climate varies from tropical in the south to arctic in the north; and natural vegetation follows the pattern of climate and altitude. Water-induced disaster problems including soil erosion, debris flow, landslides and flooding are common due to the unstable landscape. Soil erosion is the most important driving force for the degradation of upland and mountain ecosystems. Soil bioengineering has been used in Nepal for nearly 30 years to deal with erosion problems on slopes, in high way construction and riverbank stabilization. The main soil bioengineering techniques used in Nepal are brush layering, palisades, live check dams, fascines and vegetative stone pitching. This study is based on the geology, climate and vegetation of Nepal and briefly summarizes the application of soil bioengineering on slopes and stream banks, with especial attention to the role of vegetation on slope and stream bank stabilization. Furthermore, this paper addresses the role of community participation and responsibility for successful application of vegetation-based techniques in management, maintenance and utility aspects for the future. In recent years, soil bioengineering techniques are extensively used due to their cost-effectiveness, using locally available materials and low-cost labour in comparison to more elaborate civil engineering works. However, scientific implementation and record-keeping and evaluation of the work are indeed essential.

  11. Starch bioengineering

    DEFF Research Database (Denmark)

    Blennow, Andreas

    2018-01-01

    Application of starch in industry frequently requires extensive modification. This is usually achieved by chemical and/or physical modification that is time-consuming and often expensive and polluting. To impart functionality as early as possible in the starch production chain, modification can...... be achieved directly as part of the developing starch storage roots, tubers, and seeds and grains of the crop. Starch has been a strong driver for human development and is now the most important energy provider in the diet forcing the development of novel and valuable starch qualities for specific...... applications. Among the most important structures that can be targeted include starch phosphorylation chain transfer/branching generating chemically substituted and chain length-modified starches such as resistant and health-promoting high-amylose starch. Starch bioengineering has been employed for more than...

  12. Monitoring and evaluation of soil bioengineering interventions for watershed management, disaster mitigation and environmental restoration in Latin America

    Science.gov (United States)

    Petrone, Alessandro; Preti, Federico

    2013-04-01

    In recent decades the institutions responsible for land management and civil protection have showed a great interest in relation to the use of more environmentally friendly techniques to mitigate the risk of landslides and floods. Soil bioengineering has responded to this need and several research groups are carrying out experimentations using the techniques of this discipline in the countries in the developing world. The Deistaf from University of Florence has concentrated its activities in this area over the past decade promoting the use of the techniques of Soil bioengineering in Latin America through the implementation of training and experimentation programmes. Numerous works have been completed both in riverbanks and on slopes in Nicaragua, Guatemala, Ecuador and Colombia. It was decided to make a census of interventions in Latin America from different institutions that may be related to Soil bioengineering in order to obtain an overview of the state of the art in the specific context taking into account also environmental and socio-economic issues. Taking advantage of its network of contacts, DEISTAF has collected dozens of sheets that describe interventions. These sheets describe, among other fields focused on the environment in which the work has been carried out, the materials and techniques used, and the impact of the intervention. In the sheets we present also the monitoring that has been realized for some of these works in the months of October and November 2012; we include the identification of the current condition and functionality of the intervention and, in the case of the presence of some damages, the formulation of instructions to fix them as well as the economic quantification of the repairs to be carried out.

  13. 1988 Hanford riverbank springs characterization report

    International Nuclear Information System (INIS)

    Dirkes, R.L.

    1990-12-01

    This reports presents the results of a special study undertaken to characterize the riverbank springs (i.e., ground-water seepage) entering the Columbia River along the Hanford Site. Radiological and nonradiological analyses were performed. River water samples were also analyzed from upstream and downstream of the Site as well as from the immediate vicinity of the springs. In addition, irrigation return water and spring water entering the river along the shoreline opposite Hanford were analyzed. Hanford-origin contaminants were detected in spring water entering the Columbia River along the Hanford Site. The type and concentrations of contaminants in the spring water were similar to those known to exist in the ground water near the river. The location and extent of the contaminated discharges compared favorably with recent ground-water reports and predictions. Spring discharge volumes remain very small relative to the flow of the Columbia. Downstream river sampling demonstrates the impact of ground-water discharges to be minimal, and negligible in most cases. Radionuclide concentrations were below US Department of Energy Derived Concentration Guides (DCGs) with the exception 90 Sr near the 100-N Area. Tritium, while below the DCG, was detected at concentrations above the US Environmental Protection Agency drinking water standards in several springs. All other radionuclide concentrations were below drinking water standards. Nonradiological contaminants were generally undetectable in the spring water. River water contaminant concentrations, outside of the immediate discharge zones, were below drinking water standards in all cases. 19 refs., 5 figs., 12 tabs

  14. The Perspective of Riverbank Filtration in China

    Science.gov (United States)

    Li, J.; Teng, Y.; Zhai, Y.; Zuo, R.

    2014-12-01

    Sustainable drinking water supply can affect the health of people, and the surrounding ecosystems. According to statistics of the monitoring program of drinking water sources in 309 at or above prefecture level of China in 2013, the major pollutants index were total phosphorus, ammonia and manganese in surface drinking water sources, respectively, iron, ammonia and manganese in groundwater drinking water sources, respectively. More than 150 drinking water emergency environmental accidents happened since 2006, 52 of these accidents led to the disruption of water supply in waterworks, and a population of over ten million were affected. It indicated that there is a potential risk for people's health by the use of river water directly and it is necessary to require alternative techniques such as riverbank filtration for improving the drinking water quality. Riverbank filtration is an inexpensive natural process, not only smoothing out normal pollutant concentration found in surface water but also significantly reducing the risk from such emergency events as chemical spill into the river. Riverbank filtration technique has been used in many countries more than 100 years, including China. In China, in 1950s, the bank infiltration technique was first applied in northeast of China. Extensive bank infiltration application was conducted in 1980s, and more than 300 drinking water sources utilities bank infiltration established mainly near the Songhua River Basin, the Yellow River Basin, Haihe River Basin. However, the comparative lack of application and researches on riverbank filtration have formed critical scientific data gap in China. As the performance of riverbank filtration technique depend on not only the design and setting such as well type, pumping rate, but also the local hydrogeology and environmental properties. We recommend more riverbank filtration project and studies to be conducted to collect related significant environmental geology data in China

  15. Bioengineering a conceptual approach

    CERN Document Server

    Pavlovic, Mirjana

    2015-01-01

    This book explores critical principles and new concepts in bioengineering, integrating the biological, physical and chemical laws and principles that provide a foundation for the field. Both biological and engineering perspectives are included, with key topics such as the physical-chemical properties of cells, tissues and organs; principles of molecules; composition and interplay in physiological scenarios; and the complex physiological functions of heart, neuronal cells, muscle cells and tissues. Chapters evaluate the emerging fields of nanotechnology, drug delivery concepts, biomaterials, and regenerative therapy. The leading individuals and events are introduced along with their critical research. Bioengineering: A Conceptual Approach is a valuable resource for professionals or researchers interested in understanding the central elements of bioengineering. Advanced-level students in biomedical engineering and computer science will also find this book valuable as a secondary textbook or reference.

  16. Ground beetle habitat templets and riverbank integrity

    OpenAIRE

    Van Looy, Kris; Vanacker, Stijn; Jochems, Hans; De Blust, Geert; Dufrêne, M

    2006-01-01

    The habitat templet approach was used in a scale-sensitive bioindicator assessment for the ecological integrity of riverbanks and for specific responses to river management. Ground beetle habitat templets were derived from a catchment scale sampling, integrating the overall variety of bank types. This coarse-filter analysis was integrated in the reach scale fine-filtering approaches of community responses to habitat integrity and river management impacts. Higher species diversity was associat...

  17. Bioengineering fluid mechanics

    CERN Document Server

    Hung, Tin-kan

    2013-01-01

    This book highlights the basic concepts and equations for bioengineering flow processes. Physical concepts and meanings are emphasized while rigorous derivations are simplified, making it easier for self learning on some biological and medical flow processes. The well known Bernoulli equation in hydraulics is extended for pulsating flows, peristaltic flows and cardiac pumping. The dimensional analysis, model law and dimensionless equations can be related to computational models and experimental observations. The velocity vector imaging stored in echocardiograms can be used to analyze the pumping characteristics of the ventricular contraction. New topics included oxygen transport in membrane oxygenator and micro mixing of blood flow in capillary channels.

  18. Bioengineering Technology to Control River Soil Erosion using Vetiver (Vetiveria Zizaniodes)

    Science.gov (United States)

    Sriwati, M.; Pallu, S.; Selintung, M.; Lopa, R.

    2018-04-01

    Erosion is the action of surface processes (such as water flow or wind) that removes soil, rock or dissolved material from one location on the earth’s crust, and then transport it away to another location. Bioengineering is an attempt to maximise the use of vegetation components along riverbanks to cope with landslides and erosion of river cliffs and another riverbank damage. This study aims to analyze the bioengineering of Vetiver as a surface layer for soil erosion control using slope of 100, 200, and 300. This study is conducted with 3 variations of rain intensity (I), at 103 mm/hour, 107 mm/hour, and 130 mm/hour by using rainfall simulator tool. In addition, the USLE (Universal Soil Loss Equation) method is used in order to measure the rate of soil erosion. In this study, there are few USLE model parameters were used such as rainfall erosivity factor, soil erodibility factor, length-loss slope and stepness factor, cover management factor, and support practise factor. The results demonstrated that average of reduction of erosion rate using Vetiver, under 3 various rainfalls, namely rainfall intensity 103 mm/hr had reduced 84.971%, rainfall intensity 107 mm/hr had reduced 86.583 %, rainfall intensity 130 mm/hr had reduced 65.851%.

  19. Future cereal starch bioengineering

    DEFF Research Database (Denmark)

    Blennow, Andreas; Jensen, Susanne Langgård; Shaik, Shahnoor Sultana

    2013-01-01

    The importance of cereal starch production worldwide cannot be overrated. However, the qualities and resulting values of existing raw and processed starch do not fully meet future demands for environmentally friendly production of renewable, advanced biomaterials, functional foods, and biomedical...... additives. New approaches for starch bioengineering are needed. In this review, we discuss cereal starch from a combined universal bioresource point of view. The combination of new biotechniques and clean technology methods can be implemented to replace, for example, chemical modification. The recently...... released cereal genomes and the exploding advancement in whole genome sequencing now pave the road for identifying new genes to be exploited to generate a multitude of completely new starch functionalities directly in the cereal grain, converting cereal crops to production plants. Newly released genome...

  20. Topics of Bioengineering in Wikipedia

    Directory of Open Access Journals (Sweden)

    Vassia Atanassova

    2009-10-01

    Full Text Available The present report aims to give a snapshot of how topics from the field of bioengineering (bioinformatics, bioprocess systems, biomedical engineering, biotechnology, etc. are currently covered in the free electronic encyclopedia Wikipedia. It also offers insights and information about what Wikipedia is, how it functions, how and when to cite Wikipedian articles, if necessary. Several external wikis, devoted to topics of bioengineering, are also listed and reviewed.

  1. Integrative Bioengineering Institute

    Energy Technology Data Exchange (ETDEWEB)

    Eddington, David; Magin,L,Richard; Hetling, John; Cho, Michael

    2009-01-09

    Microfabrication enables many exciting experimental possibilities for medicine and biology that are not attainable through traditional methods. However, in order for microfabricated devices to have an impact they must not only provide a robust solution to a current unmet need, but also be simple enough to seamlessly integrate into standard protocols. Broad dissemination of bioMEMS has been stymied by the common aim of replacing established and well accepted protocols with equally or more complex devices, methods, or materials. The marriage of a complex, difficult to fabricate bioMEMS device with a highly variable biological system is rarely successful. Instead, the design philosophy of my lab aims to leverage a beneficial microscale phenomena (e.g. fast diffusion at the microscale) within a bioMEMS device and adapt to established methods (e.g. multiwell plate cell culture) and demonstrate a new paradigm for the field (adapt instead of replace). In order for the field of bioMEMS to mature beyond novel proof-of-concept demonstrations, researchers must focus on developing systems leveraging these phenomena and integrating into standard labs, which have largely been ignored. Towards this aim, the Integrative Bioengineering Institute has been established.

  2. Riverbed Clogging and Sustainability of Riverbank Filtration

    Directory of Open Access Journals (Sweden)

    Thomas Grischek

    2016-12-01

    Full Text Available Clogging refers to a reduction of riverbed hydraulic conductivity. Due to difficulties in determining the thickness of the clogging layer, the leakage coefficient (L is introduced and used to quantify the recoverable portion of bank filtrate. L was determined at several riverbank filtration (RBF sites in field tests and using an analytical solution. Results were compared with data from similar experiments in the early 1970s and 1991–1993. In the 1980s, severe river water pollution in conjunction with high water abstraction led to partly unsaturated conditions beneath the riverbed. A leakage coefficient L of 5 × 10−7 s−1 was determined. After water quality improvement, L increased to 1–1.5 × 10−6 s−1. An alternative, cost and time efficient method is presented to estimate accurate leakage coefficients. The analytical solution is based on groundwater level monitoring data from observation wells next to the river, which can later feed into numerical models. The analytical approach was able to reflect long-term changes as well as seasonal variations. Recommendations for its application are given based on experience.

  3. Efektifitas Riverbank Filtration Trhadap Parameter Fisik (TDS di Sungai Cihideung

    Directory of Open Access Journals (Sweden)

    Roh Santoso Budi Waspodo

    2013-01-01

    Full Text Available Water is a natural resource that is necessary for the livelihood of many people, even by all living things. Data Ministry of Public Works in 2006 mentions the availability of water in Indonesia amounted to 15,500 cubic meters per capita per year, far higher than the global availability averaged only 600 m3. If drawn, the amount of water covers 21% of Indonesia Pacific Ocean. However, the overflow Indonesia does not necessarily solve the problem of water crisis which is also predicted to fall on two major islands in 2015. The general objective of this research is to develop and implement technological innovations RBF (Riverbank Filtration to support the needs of irrigation water Watershed Cisadane, making RBF (Riverbank Filtration as a natural water treatment in Cisadane River area, and water quality data information through RBF (Riverbank Filtration Cisadane Watershed.

  4. Bioengineered anterior cruciate ligament

    Science.gov (United States)

    Martin, Ivan (Inventor); Altman, Gregory (Inventor); Kaplan, David (Inventor); Vunjak-Novakovic, Gordana (Inventor)

    2001-01-01

    The present invention provides a method for producing an anterior cruciate ligament ex vivo. The method comprises seeding pluripotent stem cells in a three dimensional matrix, anchoring the seeded matrix by attachment to two anchors, and culturing the cells within the matrix under conditions appropriate for cell growth and regeneration, while subjecting the matrix to one or more mechanical forces via movement of one or both of the attached anchors. Bone marrow stromal cells are preferably used as the pluripotent cells in the method. Suitable matrix materials are materials to which cells can adhere, such as a gel made from collagen type I. Suitable anchor materials are materials to which the matrix can attach, such as Goinopra coral and also demineralized bone. Optimally, the mechanical forces to which the matrix is subjected mimic mechanical stimuli experienced by an anterior cruciate ligament in vivo. This is accomplished by delivering the appropriate combination of tension, compression, torsion, and shear, to the matrix. The bioengineered ligament which is produced by this method is characterized by a cellular orientation and/or matrix crimp pattern in the direction of the applied mechanical forces, and also by the production of collagen type I, collagen type III, and fibronectin proteins along the axis of mechanical load produced by the mechanical forces. Optimally, the ligament produced has fiber bundles which are arranged into a helical organization. The method for producing an anterior cruciate ligament can be adapted to produce a wide range of tissue types ex vivo by adapting the anchor size and attachment sites to reflect the size of the specific type of tissue to be produced, and also adapting the specific combination of forces applied, to mimic the mechanical stimuli experienced in vivo by the specific type of tissue to be produced. The methods of the present invention can be further modified to incorporate other stimuli experienced in vivo by the

  5. Estimation of riverbank soil erodibility parameters using genetic ...

    Indian Academy of Sciences (India)

    Tapas Karmaker

    2017-11-07

    Nov 7, 2017 ... process. Therefore, this is a study to verify the applicability of inverse parameter ... successful modelling of the riverbank erosion, precise estimation of ..... For this simulation, about 40 iterations are found to attain the convergence. ..... rithm for function optimization: a Matlab implementation. NCSU-IE TR ...

  6. Impacts of extreme flooding on riverbank filtration water quality.

    Science.gov (United States)

    Ascott, M J; Lapworth, D J; Gooddy, D C; Sage, R C; Karapanos, I

    2016-06-01

    Riverbank filtration schemes form a significant component of public water treatment processes on a global level. Understanding the resilience and water quality recovery of these systems following severe flooding is critical for effective water resources management under potential future climate change. This paper assesses the impact of floodplain inundation on the water quality of a shallow aquifer riverbank filtration system and how water quality recovers following an extreme (1 in 17 year, duration >70 days, 7 day inundation) flood event. During the inundation event, riverbank filtrate water quality is dominated by rapid direct recharge and floodwater infiltration (high fraction of surface water, dissolved organic carbon (DOC) >140% baseline values, >1 log increase in micro-organic contaminants, microbial detects and turbidity, low specific electrical conductivity (SEC) 400% baseline). A rapid recovery is observed in water quality with most floodwater impacts only observed for 2-3 weeks after the flooding event and a return to normal groundwater conditions within 6 weeks (lower fraction of surface water, higher SEC, lower DOC, organic and microbial detects, DO). Recovery rates are constrained by the hydrogeological site setting, the abstraction regime and the water quality trends at site boundary conditions. In this case, increased abstraction rates and a high transmissivity aquifer facilitate rapid water quality recoveries, with longer term trends controlled by background river and groundwater qualities. Temporary reductions in abstraction rates appear to slow water quality recoveries. Flexible operating regimes such as the one implemented at this study site are likely to be required if shallow aquifer riverbank filtration systems are to be resilient to future inundation events. Development of a conceptual understanding of hydrochemical boundaries and site hydrogeology through monitoring is required to assess the suitability of a prospective riverbank filtration

  7. Bioengineering of artificial lymphoid organs

    OpenAIRE

    NOSENKO M.A.; DRUTSKAYA M.S.; MOISENOVICH M.M.; NEDOSPASOV S.A.

    2016-01-01

    This review addresses the issue of bioengineering of artificial lymphoid organs.Progress in this field may help to better understand the nature of the structure-function relations that exist in immune organs. Artifical lymphoid organs may also be advantageous in the therapy or correction of immunodefficiencies, autoimmune diseases, and cancer. The structural organization, development, and function of lymphoid tissue are analyzed with a focus on the role of intercellular contacts and on the cy...

  8. Soil bioengineering applied to the environmental rehabilitation of controlled landfills

    International Nuclear Information System (INIS)

    Luria, P.

    2005-01-01

    Soil bioengineering is a discipline characterised by the capability of associating geo-technical approaches (e.g. soil stabilisation) with naturalistic rehabilitation and creation of biotopes. It is extremely suitable for the environmental rehabilitation of controlled landfills, especially of area and depression landfills, mainly through soil protection and stabilisation measures. Its increasing notoriety is mainly due to the great variety and specificity of its techniques, to the capability of joining technical matters with naturalistic aspects, and to the reduced cost of some interventions. Nevertheless, its application to environmental rehabilitation of controlled landfills is still scarce in Italy. Only 3% of 87 closed landfills analysed, whose rehabilitation projects adopt natural techniques for soil stabilisation and protection, explicitly refers to Soil Bioengineering [it

  9. Bioengineering of Artificial Lymphoid Organs.

    Science.gov (United States)

    Nosenko, M A; Drutskaya, M S; Moisenovich, M M; Nedospasov, S A

    2016-01-01

    This review addresses the issue of bioengineering of artificial lymphoid organs.Progress in this field may help to better understand the nature of the structure-function relations that exist in immune organs. Artifical lymphoid organs may also be advantageous in the therapy or correction of immunodefficiencies, autoimmune diseases, and cancer. The structural organization, development, and function of lymphoid tissue are analyzed with a focus on the role of intercellular contacts and on the cytokine signaling pathways regulating these processes. We describe various polymeric materials, as scaffolds, for artificial tissue engineering. Finally, published studies in which artificial lymphoid organs were generated are reviewed and possible future directions in the field are discussed.

  10. THE PHILOSOPHICAL FOUNDATIONS OF MODERN BIOENGINEERING

    Directory of Open Access Journals (Sweden)

    Ibrahim A. Shogar

    2017-12-01

    Full Text Available This paper investigates the philosophical foundations of modern bioengineering to articulate its ethical framework. Engineering as an ultimate mechanism to transform knowledge into practice is essential for both physical and biological sciences. It reduces data, concepts, and designs to pictorial forms. The integration of engineering with the newly emerging biosciences, has presented a unique opportunity to overcome the major challenges that face the environmental and human health. To harness potentials of bioengineering and establish a sustainable foundation for green technology, modern scientists and engineers need to be acquainted with the normative questions of science. In addition to acquiring the general principles of scientific research and identifying the intrinsic goals of the endeavour, philosophy of bioengineering exposes bioengineers to both the descriptive ‘how’ questions of the physical world as well as the normative ‘why’ questions of values. Such an interdisciplinary approach is significant, not only for inspiring to acquire the genuine knowledge of the existing world, but also to expose the bioengineers to their ethical and social responsibilities. Besides introducing the conceptual framework of bioengineering, this paper has investigated the three major philosophies that have been dominating the theoretical presuppositions of scientific research method in history. Namely, (i Systems biology approach; (ii Evolutionary biology approach; and (iii Mechanical view approach. To establish the ethical foundation of modern bioengineering, the paper, also has conducted an analytical study on various branches of the emerging discipline of bioscience. The paper has concluded that adopting the interdisciplinary approach in research and education is essential to harness potentials of bioengineering and to establish foundations of green technology. To achieve the final objectives of bioengineering, both the practical and theoretical

  11. Transport of micropollutants in a riverbank filtration system

    Science.gov (United States)

    van Driezum, Inge; Oudega, Thomas; Reiner, Philipp; Zessner, Matthias; Farnleitner, Andreas; Blaschke, Paul

    2014-05-01

    Groundwater locations at alluvial backwaters are essential for public water supply. Riverbank filtration (RBF) systems are widely used as a means of obtaining public water supplies. Riverbank filtration is an effective way to remove micropollutants from the receiving surface water. The efficiency of the RBF system strongly depends on the residence time of the water in the aquifer and on the soil properties (Ray, 2011). In order to understand all bio- and geochemical processes within the hyporheic zone (e.g. the region were mixing of surface water and groundwater occurs), exchange rates and flow patterns need to be quantified. The main study area covers the porous groundwater aquifer study site (PGWA) - an urban floodplain extending on the left bank of the River Danube downstream of the City of Vienna. It is one of the main groundwater bodies in Austria. Groundwater quality in the PGWA is influenced by a combination of anthropogenic activities, industry, wastewater treatment plants, heavy precipitation events and floodings. The upper layer of the DPA is impermeable, preventing pollution originating from the surface. The upper layer consists of silt. The underlying confined aquifer consists of sand and gravel layers. Hydraulic conductivities range from 5 x 10-2 m/s up to 5 x 10-5 m/s. Underneath the aquifer are alternating sand an clay/silt layers. Samples are taken from two transects in the DPA. These transects consist of four piezometers in the first few meters of the groundwater aquifer. Several other piezometers are placed downstream from the river-groundwater interface. The behaviour of the micropollutants in the hyporheic zone can therefore be studied intensively. The transport behaviour of several micropollutants is modeled using carbamazepine (CBZ) and acesulfame (ACE) as natural tracers. Furthermore, temperature and electrical conductivity data was used for modeling. The micropollutants are measured using an in house developed online SPE-HPLC-MS/MS method

  12. Bioengineering and Cybernetics: A Modern Caduceus.

    Science.gov (United States)

    Magin, Richard L

    2017-01-01

    Like the caduceus, a medical symbol of entwined serpents, bioengineering and cybernetics have interwoven together ideas and concepts for over 50 years. Half a century is a long time, and whether we are talking about an academic discipline, our lives, or an old car, achieving 50 is a number that brings pause to the conversation. In books, wine, or collectibles, 50 years is termed vintage, which carries the connotation of depth and maturity. Certainly, in the case of the discipline of bioengineering, 50 years is a milestone of growth and development. By all academic measures (number of departments, current enrollment and graduates, size of faculty, and impact factor for its publications), bioengineering is a mature discipline. Presently, there are almost 100 ABET-certified bioengineering degree programs in the United States alone.

  13. Monitoring of bioengineering stabilization projects : final report.

    Science.gov (United States)

    2009-08-01

    Four sites with bioengineered stream banks were monitored for four years. Observations included quantitative : measurements of stream discharge, stage and velocity. No bank erosion was observed to have affected the stream : banks at any of the monito...

  14. Monitoring Riverbank Erosion in Mountain Catchments Using Terrestrial Laser Scanning

    Directory of Open Access Journals (Sweden)

    Laura Longoni

    2016-03-01

    Full Text Available Sediment yield is a key factor in river basins management due to the various and adverse consequences that erosion and sediment transport in rivers may have on the environment. Although various contributions can be found in the literature about sediment yield modeling and bank erosion monitoring, the link between weather conditions, river flow rate and bank erosion remains scarcely known. Thus, a basin scale assessment of sediment yield due to riverbank erosion is an objective hard to be reached. In order to enhance the current knowledge in this field, a monitoring method based on high resolution 3D model reconstruction of riverbanks, surveyed by multi-temporal terrestrial laser scanning, was applied to four banks in Val Tartano, Northern Italy. Six data acquisitions over one year were taken, with the aim to better understand the erosion processes and their triggering factors by means of more frequent observations compared to usual annual campaigns. The objective of the research is to address three key questions concerning bank erosion: “how” erosion happens, “when” during the year and “how much” sediment is eroded. The method proved to be effective and able to measure both eroded and deposited volume in the surveyed area. Finally an attempt to extrapolate basin scale volume for bank erosion is presented.

  15. Bioengineering thermodynamics of biological cells.

    Science.gov (United States)

    Lucia, Umberto

    2015-12-01

    Cells are open complex thermodynamic systems. They can be also regarded as complex engines that execute a series of chemical reactions. Energy transformations, thermo-electro-chemical processes and transports phenomena can occur across the cells membranes. Moreover, cells can also actively modify their behaviours in relation to changes in their environment. Different thermo-electro-biochemical behaviours occur between health and disease states. But, all the living systems waste heat, which is no more than the result of their internal irreversibility. This heat is dissipated into the environment. But, this wasted heat represent also a sort of information, which outflows from the cell toward its environment, completely accessible to any observer. The analysis of irreversibility related to this wasted heat can represent a new approach to study the behaviour of the cells themselves and to control their behaviours. So, this approach allows us to consider the living systems as black boxes and analyze only the inflows and outflows and their changes in relation to the modification of the environment. Therefore, information on the systems can be obtained by analyzing the changes in the cell heat wasted in relation to external perturbations. The bioengineering thermodynamics bases are summarized and used to analyse possible controls of the calls behaviours based on the control of the ions fluxes across the cells membranes.

  16. Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.

    Science.gov (United States)

    Takagi, Ryoji; Ishimaru, Junko; Sugawara, Ayaka; Toyoshima, Koh-Ei; Ishida, Kentaro; Ogawa, Miho; Sakakibara, Kei; Asakawa, Kyosuke; Kashiwakura, Akitoshi; Oshima, Masamitsu; Minamide, Ryohei; Sato, Akio; Yoshitake, Toshihiro; Takeda, Akira; Egusa, Hiroshi; Tsuji, Takashi

    2016-04-01

    The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.

  17. Bio-engineering for land stabilization : executive summary report.

    Science.gov (United States)

    2010-06-30

    Soil-bioengineering, or simply : bioengineering, is the use of vegetation for : slope stabilization. Currently, a large : number of slopes near Ohio highways are : experiencing stability problems. These : failures usually begin as local erosion...

  18. The Status of Fluid Mechanics in Bioengineering Curricula.

    Science.gov (United States)

    Miller, Gerald E.; Hyman, William A.

    1981-01-01

    Describes the status of fluid mechanics courses in bioengineering curricula. A survey of institutions offering bioengineering degrees indicates that over half do not require fluid mechanics courses. Suggests increasing number of mechanics courses to increase the quality of bioengineering students and to prepare students for graduate work and more…

  19. Riverbank Filtration: A Sustainable Process to Attenuate Contaminants during Drinking Water Production

    Directory of Open Access Journals (Sweden)

    Pradeep Kumar

    2018-03-01

    Full Text Available Riverbank filtration leads to purification of water. For India it can be a simple, economical and effective alternative. A few unanswered questions were: Can it work in Indian mountainous regions? Will it be of any advantage in the case of some of the polluted Indian surface waters? With the goal to evaluate use of riverbank filtration as a sustainable technology under widely varying conditions prevalent in India, the effectiveness of riverbank filtration has been examined over the last 10 years. In the case of cleaner surface waters, the wells deliver water free of turbidity and coliform even during monsoon irrespective of well configuration. In the case of polluted source waters, it results in an overall advantage in terms of improved raw water quality, reduced degree and cost of subsequent treatment and decreased levels of disinfection by-products. The study shows riverbank filtration to be an effective and sustainable option for plains as well as the mountainous region.

  20. Bio-engineering in the Baltic Sea

    DEFF Research Database (Denmark)

    Zandersen, Marianne

    2011-01-01

    Bio-engineering in the Baltic Sea – value of water quality improvements & risk perceptions Dr. Marianne Zandersen1 Department of Environmental Science, Aarhus University Abstract The Baltic Sea is heavily eutrofied and the trend has gone from bad to worse. The hypoxic zone has increased about 4...... of the water column to the bottom waters/deepwater. The expected effects include a slowing down of the sediment release from the bottom and improvement of the possibilities for aerobic bacterial decomposition and over time for the establishment of fauna. The projects test a bio-engineered approach to speeding...

  1. Integrated electronics and fluidic MEMS for bioengineering

    Science.gov (United States)

    Fok, Ho Him Raymond

    pedestals. This approach also allows the in-vitro model to mimic the three-dimensionality of the cellular mitotic spindle that is absent in previous work. The second application of an integrated bioengineering system discussed in this dissertation is to design and fabricate active electronics and sensors for an in-vivo application to monitor neural activity at the cellular level. Temperature sensors were chosen for a first demonstration. In order for temperature sensors to be able to be implanted into brain interfaces, it is necessary for these devices to be fabricated using processes that are compatible with bio-compatible substrates such as glass and plastic. This dissertation addresses this challenge by developing temperature sensors integrated with biasing circuitry using zinc oxide thin film transistors (TFTs) fabricated on polyimide substrates. The integrated sensors show good temperature sensitivity, which is critical for monitoring neural temperature at the cellular level. This dissertation also describes the unique requirements of encapsulating implantable electronics. For instance, encapsulation schemes must be designed in such a way that they both protect electronic devices from extracellular fluids and also do not interfere with the functionality of these devices. In this work, SU-8 is used as a convenient and effective encapsulation layer. Thermal engineering to prevent active electronics from overheating and to ensure accurate temperature measurement from temperature sensors is also discussed, and a synergistic encapsulation and thermal engineering combination is presented.

  2. Bioengineering towards self-assembly of particulate vaccines.

    Science.gov (United States)

    Rehm, Bernd H A

    2017-12-01

    There is an unmet demand for safe and efficient vaccines for prevention of various infectious diseases. Subunit vaccines comprise selected pathogen specific antigens are a safe alternative to whole organism vaccines. However they often lack immunogenicity. Natural and synthetic self-assembling polymers and proteins will be reviewed in view their use to encapsulate and/or display antigens to serve as immunogenic antigen carriers for induction of protective immunity. Recent advances made in in vivo assembly of antigen-displaying polyester inclusions will be a focus. Particulate vaccines are inherently immunogenic due to enhanced uptake by antigen presenting cells which process antigens mediating adaptive immune responses. Bioengineering approaches enable the design of tailor-made particulate vaccines to fine tune immune responses towards protective immunity. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Scales of form roughness on riverbanks with different riparian vegetation

    Science.gov (United States)

    Konsoer, K. M.; Rhoads, B. L.; Best, J.; Langendoen, E. J.; Ursic, M.; Abad, J. D.; Garcia, M. H.

    2013-12-01

    Riverbanks often include topographic irregularities that occur over a range of scales and that are produced by interactions among erosional processes, vegetation, and the geotechnical properties of the banks and floodplains. Irregularity of the bank surface can increase form drag, affecting the overall flow resistance, near-bank shear stresses, and patterns of sediment transport. Understanding how dominant scales of form roughness influence the near-bank flow structure, and thus the shear stress partitioning, is vital for the development of accurate predictive morphodynamic models. In this paper, the scales of bank roughness are examined for two meander bends of a large alluvial river with differing riparian vegetation on the Wabash River near Grayville, Illinois. Detailed measurements of bank topography were obtained using terrestrial LiDAR during low flow events and a multibeam echo sounder (MBES) during bankfull events. These measurements yielded high spatial resolution maps (~5-10 cm) that were used to analyze scales of roughness at different elevations along the banks during both subaerial and subaqueous conditions. The results of these analyses provide insight into the influence of riparian vegetation on form roughness and patterns of near-bank flow structure as documented using acoustic Doppler current profilers (ADCP).

  4. Flow Hydrodynamics across Open Channel Flows with Riparian Zones: Implications for Riverbank Stability

    Directory of Open Access Journals (Sweden)

    Da Liu

    2017-09-01

    Full Text Available Riverbank vegetation is of high importance both for preserving the form (morphology and function (ecology of natural river systems. Revegetation of riverbanks is commonly used as a means of stream rehabilitation and management of bank instability and erosion. In this experimental study, the effect of different riverbank vegetation densities on flow hydrodynamics across the channel, including the riparian zone, are reported and discussed. The configuration of vegetation elements follows either linear or staggered arrangements as vegetation density is progressively increased, within a representative range of vegetation densities found in nature. Hydrodynamic measurements including mean streamwise velocity and turbulent intensity flow profiles are recorded via acoustic Doppler velocimetry (ADV—both at the main channel and within the riverbank. These results show that for the main channel and the toe of riverbank, turbulence intensity for the low densities (λ ≈ 0 to 0.12 m−1 can increase up to 40% compared the case of high densities (λ = 0.94 to 1.9 m−1. Further analysis of these data allowed the estimation of bed-shear stresses, demonstrating 86% and 71% increase at the main channel and near the toe region, for increasing densities (λ = 0 to 1.9 m−1. Quantifying these hydrodynamic effects is important for assessing the contribution of physically representative ranges of riparian vegetation densities on hydrogeomorphologic feedback.

  5. Application of short-range photogrammetry for monitoring seepage erosion of riverbank by laboratory experiments

    Science.gov (United States)

    Masoodi, A.; Noorzad, A.; Majdzadeh Tabatabai, M. R.; Samadi, A.

    2018-03-01

    Temporal and spatial monitoring play a significant role in evaluating and examining the riverbank morphology and its spatiotemporal changes. Unlike the terrestrial laser scanners, other previously used methods such as satellite images, total station surveying, and erosion pins have limited application to quantify the small-scale bank variations due to the lack of rapid survey and resolution in data acquisition. High cost, lack of availability, specialized equipment and hard movement of laser scanners make it necessary to develop new accurate, economical and easily available methods. The present study aims to test the Kinect photogrametric technology for measuring and assessing riverbank variations in laboratory environment. For this purpose, three models of layered soil blocks for three different levels of groundwater (i.e. 24, 34 and 44 cm) were designed to investigate the seepage erosion behavior experimentally. The results indicate the high accuracy of Kinect in measuring the bank erosion cavity dimensions (i.e., 0.5% error) with high spatial resolution data (i.e. 300,000 points per frame). The high speed of Kinect in riverbank scanning enables the analysis of time variations of mechanisms such as seepage erosion which occurs rather rapidly. The results confirmed that there is a power relationship between the seepage gradient and the time of the bank failure with a determination coefficient of 0.97. Moreover, an increase in the level of groundwater on the riverbank increases the rate of undercutting retreat that caused more rapid failure of the riverbank.

  6. Bioengineering Strategies to Treat Female Infertility.

    Science.gov (United States)

    Kuo, Che-Ying; Baker, Hannah; Fries, Melissa H; Yoo, James J; Kim, Peter C W; Fisher, John P

    2017-06-01

    Bioengineering strategies have demonstrated enormous potential to treat female infertility as a result of chemotherapy, uterine injuries, fallopian tube occlusion, massive intrauterine adhesions, congenital uterine malformations, and hysterectomy. These strategies can be classified into two broad categories as follows: (i) Transplantation of fresh or cryopreserved organs into the host and (ii) tissue engineering approaches that utilize a combination of cells, growth factors, and biomaterials that leverages the body's inherent ability to regenerate/repair reproductive organs. While whole organ transplant has demonstrated success, the source of the organ and the immunogenic effects of allografts remain challenging. Even though tissue engineering strategies can avoid these issues, their feasibilities of creating whole organ constructs are yet to be demonstrated. In this article we summarize the recent advancements in the applications of bioengineering to treat female infertility.

  7. 76 FR 67174 - Arkansas Electric Cooperative Corp., Riverbank Hydro No. 9 LLC, Solia 3 Hydroelectric LLC, Lock...

    Science.gov (United States)

    2011-10-31

    ...; 14166-000; 14180-000; 14193-000] Arkansas Electric Cooperative Corp., Riverbank Hydro No. 9 LLC, Solia 3... May 2, 2011, Arkansas Electric Cooperative Corp. (Arkansas Electric), Riverbank Hydro No. 9 LLC.... Krouse, Hydro Green Energy, 5090 Richmond Avenue 390, Houston, TX 77056. (877) 556-6566 x 709. FFP 2's...

  8. Bioengineering of Artificial Antigen Presenting Cells and Lymphoid Organs.

    Science.gov (United States)

    Wang, Chao; Sun, Wujin; Ye, Yanqi; Bomba, Hunter N; Gu, Zhen

    2017-01-01

    The immune system protects the body against a wide range of infectious diseases and cancer by leveraging the efficiency of immune cells and lymphoid organs. Over the past decade, immune cell/organ therapies based on the manipulation, infusion, and implantation of autologous or allogeneic immune cells/organs into patients have been widely tested and have made great progress in clinical applications. Despite these advances, therapy with natural immune cells or lymphoid organs is relatively expensive and time-consuming. Alternatively, biomimetic materials and strategies have been applied to develop artificial immune cells and lymphoid organs, which have attracted considerable attentions. In this review, we survey the latest studies on engineering biomimetic materials for immunotherapy, focusing on the perspectives of bioengineering artificial antigen presenting cells and lymphoid organs. The opportunities and challenges of this field are also discussed.

  9. Bioengineered Lacrimal Gland Organ Regeneration in Vivo

    Directory of Open Access Journals (Sweden)

    Masatoshi Hirayama

    2015-07-01

    Full Text Available The lacrimal gland plays an important role in maintaining a homeostatic environment for healthy ocular surfaces via tear secretion. Dry eye disease, which is caused by lacrimal gland dysfunction, is one of the most prevalent eye disorders and causes ocular discomfort, significant visual disturbances, and a reduced quality of life. Current therapies for dry eye disease, including artificial tear eye drops, are transient and palliative. The lacrimal gland, which consists of acini, ducts, and myoepithelial cells, develops from its organ germ via reciprocal epithelial-mesenchymal interactions during embryogenesis. Lacrimal tissue stem cells have been identified for use in regenerative therapeutic approaches aimed at restoring lacrimal gland functions. Fully functional organ replacement, such as for tooth and hair follicles, has also been developed via a novel three-dimensional stem cell manipulation, designated the Organ Germ Method, as a next-generation regenerative medicine. Recently, we successfully developed fully functional bioengineered lacrimal gland replacements after transplanting a bioengineered organ germ using this method. This study represented a significant advance in potential lacrimal gland organ replacement as a novel regenerative therapy for dry eye disease. In this review, we will summarize recent progress in lacrimal regeneration research and the development of bioengineered lacrimal gland organ replacement therapy.

  10. Introduction to bioengineering: melding of engineering and biological sciences.

    Science.gov (United States)

    Shoureshi, Rahmat A

    2005-04-01

    Engineering has traditionally focused on the external extensions of organisms, such as transportation systems, high-rise buildings, and entertainment systems. In contrast, bioengineering is concerned with inward processes of biologic organisms. Utilization of engineering principles and techniques in the analysis and solution of problems in medicine and biology is the basis for bioengineering. This article discusses subspecialties in bioengineering and presents examples of projects in this discipline.

  11. A Combined Approach to Measure Micropollutant Behaviour during Riverbank Filtration

    Science.gov (United States)

    van Driezum, Inge; Saracevic, Ernis; Derx, Julia; Kirschner, Alexander; Sommer, Regina; Farnleitner, Andreas; Blaschke, Alfred Paul

    2016-04-01

    Riverbank filtration (RBF) systems are widely used as natural treatment process. The advantages of RBF over surface water abstraction are the elimination of for example suspended solids, biodegradable compounds (like specific micropollutants), bacteria and viruses (Hiscock and Grischek, 2002). However, in contrast to its importance, remarkably less is known on the respective external (e.g. industrial or municipal sewage) and the internal (e.g. wildlife and agricultural influence) sources of contaminants, the environmental availability and fate of the various hazardous substances, and its potential transport during soil and aquifer passage. The goal of this study is to get an insight in the behaviour of various micropollutants and microbial indicators during riverbank filtration. Field measurements were combined with numerical modelling approaches. The study area comprises an alluvial backwater and floodplain area downstream of Vienna. The river is highly dynamic, with discharges ranging from 900 m3/s during low flow to 11000 m3/s during flood events. Samples were taken in several monitoring wells along a transect extending from the river towards a backwater river in the floodplain. Three of the piezometers were situated in the first 20 meters away from the river in order to obtain information about micropollutant behaviour close to the river. A total of 9 different micropollutants were analysed in grab samples taken under different river flow conditions (n=33). Following enrichment using SPE, analysis was performed using high performance liquid chromatography-tandem mass spectrometry. Faecal indicators (E. coli and enterococci) and bacterial spores were enumerated in sample volumes of 1 L each using cultivation based methods (ISO 16649-1, ISO 7899-2:2000 and ISO 6222). The analysis showed that some compounds, e.g. ibuprofen and diclofenac, were only found in the river. These compounds were already degraded in the first ten meters away from the river. Analysis of

  12. Riverbank filtration for the treatment of highly turbid Colombian rivers

    Science.gov (United States)

    Gutiérrez, Juan Pablo; van Halem, Doris; Rietveld, Luuk

    2017-05-01

    The poor quality of many Colombian surface waters forces us to seek alternative, sustainable treatment solutions with the ability to manage peak pollution events and to guarantee the uninterrupted provision of safe drinking water to the population. This review assesses the potential of using riverbank filtration (RBF) for the highly turbid and contaminated waters in Colombia, emphasizing water quality improvement and the influence of clogging by suspended solids. The suspended sediments may be favorable for the improvement of the water quality, but they may also reduce the production yield capacity. The cake layer must be balanced by scouring in order for an RBF system to be sustainable. The infiltration rate must remain high enough throughout the river-aquifer interface to provide the water quantity needed, and the residence time of the contaminants must be sufficient to ensure adequate water quality. In general, RBF seems to be a technology appropriate for use in highly turbid and contaminated surface rivers in Colombia, where improvements are expected due to the removal of turbidity, pathogens and to a lesser extent inorganics, organic matter and micro-pollutants. RBF has the potential to mitigate shock loads, thus leading to the prevention of shutdowns of surface water treatment plants. In addition, RBF, as an alternative pretreatment step, may provide an important reduction in chemical consumption, considerably simplifying the operation of the existing treatment processes. However, clogging and self-cleansing issues must be studied deeper in the context of these highly turbid waters to evaluate the potential loss of abstraction capacity yield as well as the development of different redox zones for efficient contaminant removal.

  13. LEAKAGE CHARACTERISTICS OF BASE OF RIVERBANK BY SELF POTENTIAL METHOD AND EXAMINATION OF EFFECTIVENESS OF SELF POTENTIAL METHOD TO HEALTH MONITORING OF BASE OF RIVERBANK

    Science.gov (United States)

    Matsumoto, Kensaku; Okada, Takashi; Takeuchi, Atsuo; Yazawa, Masato; Uchibori, Sumio; Shimizu, Yoshihiko

    Field Measurement of Self Potential Method using Copper Sulfate Electrode was performed in base of riverbank in WATARASE River, where has leakage problem to examine leakage characteristics. Measurement results showed typical S-shape what indicates existence of flow groundwater. The results agreed with measurement results by Ministry of Land, Infrastructure and Transport with good accuracy. Results of 1m depth ground temperature detection and Chain-Array detection showed good agreement with results of the Self Potential Method. Correlation between Self Potential value and groundwater velocity was examined model experiment. The result showed apparent correlation. These results indicate that the Self Potential Method was effective method to examine the characteristics of ground water of base of riverbank in leakage problem.

  14. Liver bioengineering: Current status and future perspectives

    Institute of Scientific and Technical Information of China (English)

    Christopher Booth; Tom Soker; Pedro Baptista; Christina L Ross; Shay Soker; Umar Farooq; Robert J Stratta

    2012-01-01

    The present review aims to illustrate the strategies that are being implemented to regenerate or bioengineer livers for clinical purposes.There are two general pathways to liver bioengineering and regeneration.The first consists of creating a supporting scaffold,either synthetically or by decellularization of human or animal organs,and seeding cells on the scaffold,where they will mature either in bioreactors or in vivo.This strategy seems to offer the quickest route to clinical translation,as demonstrated by the development of liver organoids from rodent livers which were repopulated with organ specific cells of animal and/or human origin.Liver bioengineering has potential for transplantation and for toxicity testing during preclinical drug development.The second possibility is to induce liver regeneration of dead or resected tissue by manipulating cell pathways.In fact,it is well known that the liver has peculiar regenerative potential which allows hepatocyte hyperplasia after amputation of liver volume.Infusion of autologous bone marrow cells,which aids in liver regeneration,into patients was shown to be safe and to improve their clinical condition,but the specific cells responsible for liver regeneration have not yet been determined and the underlying mechanisms remain largely unknown.A complete understanding of the cell pathways and dynamics and of the functioning of liver stem cell niche is necessary for the clinical translation of regenerative medicine strategies.As well,it will be crucial to elucidate the mechanisms through which cells interact with the extracellular matrix,and how this latter supports and drives cell fate.

  15. Bioengineered yogurt: ‘open source’ medicine

    Directory of Open Access Journals (Sweden)

    Shireesh Apte

    2012-12-01

    Full Text Available The ease with which commensal bacteria in yogurt can be bioengineered to express an array of biotherapeutics, coupled with altruistic distribution systems that can easily include those, most likely to benefit (think expression of antiplasmodial peptides in the Bifidobacterium genus so that a herder in the Sahel can inoculate this yogurt culture into goat milk thus making ‘antimalarial yogurt’ for free for his entire village. This could potentially go on as long as the plasmid is stable and could lead to a revolution in the way medicines could become ‘open sourced’.

  16. Design Criteria in Revitalizing Old Warehouse District on the Kalimas Riverbank Area of Surabaya City

    Directory of Open Access Journals (Sweden)

    Endang Titi Sunarti Darjosanjoto

    2015-09-01

    Full Text Available Neglected warehouse buildings along the Kalimas River have created a poor urban façade in terms of visual quality. However the city government is planning to encourage tourism activities that take advantage of Kalimas River and its surrounding environment. If there is no good plan in accordance with the concept of local identity for old city of Surabaya, it will reduce it as a tourist attraction. In reference to the issue above, design criteria needs to be compiled for revitalizing the old warehouse district, which is expected to revive the identity of this district and be able to support the city’s tourism. This study was conducted by recording field observations, and the data was analyzed using the character appraisal method. The character appraisal analysis method is presented in the form of street picture data, which is divided into determined segments. The results show that there are five components including place attachment, sustainable urban design, green open space design, ecological riverfront design, and activity support that should be considered in the revitalization of the warehouse district. Those components are divided into two parts: building and open space at the riverbank. There are 13 design criteria for building at the riverbank, while there are 14 design criteria for open space at the riverbank. These design criteria can enrich the warehouse district’s revitalization by improving the visual quality of the urban environment.Keywords: design criteria; warehouse district; riverbank; Surabaya; revitalization.

  17. Environmental Life Cycle Assessment Model for Soil Bioengineering Measures on Infrastructure Slopes

    Science.gov (United States)

    Hoerbinger, Stephan; Obriejetan, Michael

    2015-04-01

    used. The principle of biological engineering constructions is based on the combination of dead and living materials and the emerging positive synergistic effects. The dead auxiliaries (stone, wood, etc.) protect the living plants until they undertake technical functions. During the operational phase of the structure, maintenance work has to be done in order to maintain the protective properties of the used plants. Through measures like cutting the vegetation back to the trunk, energy will be put into the system (e.g. through machine use), but will also be put out in form of biomass. Additionally, carbon is sequestered by the biomass production of the plants. The end of life phase is reached after the projected lifetime of a structure. Outside of zones with plant growth restrictions like within a clearance gauge, soil bioengineering approaches are designed to have a subsequent use phase. That means, that after the actual lifespan of the construction, a forest should have developed and maintain its balance by natural succession. The LCA model aims at supporting sustainable management strategies for CED and GWP as a base for Green Public Procurement and eco- friendly implementation and maintenance of soil bioengineering structures on infrastructure slopes.

  18. 7th WACBE World Congress on Bioengineering 2015

    CERN Document Server

    Lim, Chwee

    2015-01-01

    This volume publishes the proceedings of the WACBE World Congress on Bioengineering 2015 (WACBE 2015), which was be held in Singapore, from 6 to 8 July 2015. The World Association for Chinese Biomedical Engineers (WACBE) organizes this World Congress biannually. Our past congresses have brought together many biomedical engineers from over the world to share their experiences and views on the future development of biomedical engineering. The 7th WACBE World Congress on Bioengineering 2015 in Singapore continued to offer such a networking platform for all biomedical engineers. Hosted by the Biomedical Engineering Society (Singapore) and the Department of Biomedical Engineering, National University of Singapore, the congress covered all related areas in bioengineering.

  19. Synthetic biology: Emerging bioengineering in Indonesia

    Science.gov (United States)

    Suhandono, Sony

    2017-05-01

    The development of synthetic biology will shape the new era of science and technology. It is an emerging bioengineering technique involving genetic engineering which can alter the phenotype and behavior of the cell or the new product. Synthetic biology may produce biomaterials, drugs, vaccines, biosensors, and even a recombinant secondary metabolite used in herbal and complementary medicine, such as artemisinin, a malaria drug which is usually extracted from the plant Artemisia annua. The power of synthetic biology has encouraged scientists in Indonesia, and is still in early development. This paper also covers some research from an Indonesian research institute in synthetic biology such as observing the production of bio surfactants and the enhanced production of artemisinin using a transient expression system. Synthetic biology development in Indonesia may also be related to the iGEM competition, a large synthetic biology research competition which was attended by several universities in Indonesia. The application of synthetic biology for drug discovery will be discussed.

  20. The bioengineered kidney: science or science fiction?

    Science.gov (United States)

    Oxburgh, Leif; Carroll, Thomas J

    2016-07-01

    This article gives an overview of important new advances relating to kidney bioengineering. Directed differentiation studies have shown that proximal tubules, distal tubules, podocytes, collecting ducts, interstitium and endothelial cells can be generated from patient-derived stem cells using standardized protocols. One caveat to the interpretation of these studies is that the physiological characteristics of differentiated cells remain to be defined. Another important area of progress is scaffolding. Both decellularized organs and polymeric materials are being used as platforms for three-dimensional growth of kidney tissue, and key distinctions between these approaches are discussed. In the past 3 years, it has become clear that building kidney tissue is feasible. The laboratory-grown kidney is an attainable goal if efforts are focused on refining directed differentiation procedures to optimize cell function and on developing scaffolding strategies that ensure physiological function at the tissue level.

  1. A specialized bioengineering ion beam line

    International Nuclear Information System (INIS)

    Yu, L.D.; Sangyuenyongpipat, S.; Sriprom, C.; Thongleurm, C.; Suwanksum, R.; Tondee, N.; Prakrajang, K.; Vilaithong, T.; Brown, I.G.; Wiedemann, H.

    2007-01-01

    A specialized bioengineering ion beam line has recently been completed at Chiang Mai University to meet rapidly growing needs of research and application development in low-energy ion beam biotechnology. This beam line possesses special features: vertical main beam line, low-energy (30 keV) ion beams, double swerve of the beam, a fast pumped target chamber, and an in-situ atomic force microscope (AFM) system chamber. The whole beam line is situated in a bioclean environment, occupying two stories. The quality of the ion beam has been studied. It has proved that this beam line has significantly contributed to our research work on low-energy ion beam biotechnology

  2. Evaluating PHA productivity of bioengineered Rhodosprillum rubrum.

    Directory of Open Access Journals (Sweden)

    Huanan Jin

    Full Text Available This study explored the potential of using Rhodosprillum rubrum as the biological vehicle to convert chemically simple carbon precursors to a value-added bio-based product, the biopolymer PHA. R. rubrum strains were bioengineered to overexpress individually or in various combinations, six PHA biosynthetic genes (phaC1, phaA, phaB, phaC2, phaC3, and phaJ, and the resulting nine over-expressing strains were evaluated to assess the effect on PHA content, and the effect on growth. These experiments were designed to genetically evaluate: 1 the role of each apparently redundant PHA polymerase in determining PHA productivity; 2 identify the key gene(s within the pha biosynthetic operon that determines PHA productivity; and 3 the role of phaJ to support PHA productivity. The result of overexpressing each PHA polymerase-encoding gene indicates that phaC1 and phaC2 are significant contributors to PHA productivity, whereas phaC3 has little effect. Similarly, over-expressing individually or in combination the three PHA biosynthesis genes located in the pha operon indicates that phaB is the key determinant of PHA productivity. Finally, analogous experiments indicate that phaJ does not contribute significantly to PHA productivity. These bioengineering strains achieved PHA productivity of up to 30% of dry biomass, which is approximately 2.5-fold higher than the non-engineered control strain, indicating the feasibility of using this approach to produce value added bio-based products.

  3. Bio-engineering for land stabilization : final report.

    Science.gov (United States)

    2010-06-01

    As part of the Ohio Department of Transportations (ODOTs) ongoing effort to solve engineering problems for the Ohio : transportation system through research, The Ohio State University has undertaken a study entitled Bioengineering for : Land...

  4. Evolution of bioengineering at UCSD: opening new vistas.

    Science.gov (United States)

    Subramaniam, Shankar

    2012-07-01

    Before this, the field of bioengineering refers to biomedical engineering of prosthetic devices in physiology. In addition to exciting applications of engineering principles, UCSD Department of Bioengineering began to extend the notion of engineering models of physiological systems to physiological processes. This led to a conceptual shift in the discipline and contributed to the areas of tissue and physiological process engineering. In 1988, Dr. Shu Chien and Richard Skalak joined UCSD to begin research and education on cellular and molecular bioengineering, especially, mechanobiology. Dr. Fung and Dr. Skalak initiated the new field of tissue engineering. These two decades of evolution of bioengineering and its growth across the country was spearheaded by the Whitaker Foundation, whose leitmotif was the building of bioand biomedical engineering across the country. We have garnered other accomplishments in the following fields: regenerative medicine; bioinspired artificial extracellular matrices; flexible bioelectronics and tatoos; cells show how to synchronize biological clocks; and systems medicine.

  5. Bioengineering case studies sustainable stream bank and slope stabilization

    CERN Document Server

    Goldsmith, Wendi; McCullah, John

    2014-01-01

    This unique volume describes and evaluates 30 projects from across the United States where bio-stabilization was employed to address a detrimental naturally occurring process or byproduct of the built environment. Bio-stabilization (or soil bioengineering) refers to the use of plant materials, primarily live cuttings, arranged in the ground in different arrays to reinforce soils and protect upland slopes and/or stream banks against surficial erosion and shallow slope failures. Examples included in the collection represent different regions of the country and their specific conditions and challenges. Each project is illustrated with a number of distinctive photographs to support the reader's understanding and showcase the wide scope of projects and techniques presented. This book also: ·         Presents a range of well-documented case studies on key techniques and best practices for bio-stabilization projects ·         Emphasizes evaluation and comparison of different techniques and challeng...

  6. Identification and analysis the illegal dumping spot of solid waste at Ciliwung segment 5 riverbanks

    Science.gov (United States)

    Indrawati, D.; Purwaningrum, P.

    2018-01-01

    Ciliwung River is the main river in the area of Jakarta that is divided into six segments across West Java and Jakarta. The study focuses on the fifth segment which is 30 km long, covering from Kelapa Dua Depok to Manggarai, South Jakarta. The survey of the river consists of 3 sub-segments: Lenteng Agung, Pejaten Timur and Manggarai. Objectives of the study are to describe the characteristics and typology of the residential surrounding the Ciliwung Segment 5 Riverbank, to identification the illegal dumping spot of solid waste, to measure the volume and composition of solid waste in the riverbank, to decide solid waste management for residential area surrounding river banks to control the river pollution. The study shows that there are 11 illegal dumping spot of solid waste consisting of 4.37 m3 solid waste volume. The average composition of solid waste consists of 44% organic, 14% woods, 12% papers, 11% plastics, 3% rubbers, 1% metals and 2% others. To control the river pollution efforts are restoring the function of riverbanks to become green open space area, installing the trash rack into the river, to manage domestic solid waste based on 3R (Reduce, Reuse, Recycle) concept.

  7. Fractional calculus in bioengineering, part 3.

    Science.gov (United States)

    Magin, Richard L

    2004-01-01

    Fractional calculus (integral and differential operations of noninteger order) is not often used to model biological systems. Although the basic mathematical ideas were developed long ago by the mathematicians Leibniz (1695), Liouville (1834), Riemann (1892), and others and brought to the attention of the engineering world by Oliver Heaviside in the 1890s, it was not until 1974 that the first book on the topic was published by Oldham and Spanier. Recent monographs and symposia proceedings have highlighted the application of fractional calculus in physics, continuum mechanics, signal processing, and electromagnetics, but with few examples of applications in bioengineering. This is surprising because the methods of fractional calculus, when defined as a Laplace or Fourier convolution product, are suitable for solving many problems in biomedical research. For example, early studies by Cole (1933) and Hodgkin (1946) of the electrical properties of nerve cell membranes and the propagation of electrical signals are well characterized by differential equations of fractional order. The solution involves a generalization of the exponential function to the Mittag-Leffler function, which provides a better fit to the observed cell membrane data. A parallel application of fractional derivatives to viscoelastic materials establishes, in a natural way, hereditary integrals and the power law (Nutting/Scott Blair) stress-strain relationship for modeling biomaterials. In this review, I will introduce the idea of fractional operations by following the original approach of Heaviside, demonstrate the basic operations of fractional calculus on well-behaved functions (step, ramp, pulse, sinusoid) of engineering interest, and give specific examples from electrochemistry, physics, bioengineering, and biophysics. The fractional derivative accurately describes natural phenomena that occur in such common engineering problems as heat transfer, electrode/electrolyte behavior, and sub

  8. Seasonal variations in composite riverbank stability in the Lower Jingjiang Reach, China

    Science.gov (United States)

    Xia, Junqiang; Zong, Quanli; Deng, Shanshan; Xu, Quanxi; Lu, Jinyou

    2014-11-01

    Bank erosion is a key process in a fluvial system in the context of river dynamics and geomorphology. Since the operation of the Three Gorges Project (TGP), the Lower Jingjiang Reach (LJR) below the dam has experienced continuous channel degradation, with the phenomenon of bank erosion occurring frequently in local reaches. Therefore it is necessary to quantitatively investigate seasonal variations in the stability of composite riverbanks along the reach in order to better understand the fluvial processes in the reach. Laboratory tests were conducted for the sampled soils at six riverbanks during a field survey, with various bank soil properties being presented for the first time. These test results show that: the cohesive bank soils are relatively loose due to the high water contents of 28.5-40.0% and the low dry densities of 1.31-1.47 tonnes/m3; and the cohesion or angle of internal friction generally decreases with an increase in water content of the cohesive soil. Based on the measured cross-sectional profiles and interpolated hydrological data, the near-bank hydrodynamic conditions and soil parameters of two typical composite riverbanks were then determined during the 2007 hydrological year. An improved method was proposed for calculating the stability at the mode of cantilever failure for the overhanging block of a composite riverbank, and the stability degrees of these two riverbanks were calculated at different stages. These results reveal that: (i) the incipient velocity of the non-cohesive lower bank had a magnitude of 0.4 m/s, less than the mean near-bank velocity of about 1.0 m/s, which led to intensive basal erosion especially during the flood season; (ii) the cohesive upper bank before failure had sufficient strength to resist direct fluvial erosion, but the failed soil mass deposited in the near-bank zone was disintegrated easily with the submerged immersion and was then transported downstream by fluvial entrainment; (iii) the degree of bank

  9. Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model

    OpenAIRE

    Takagi, Ryoji; Ishimaru, Junko; Sugawara, Ayaka; Toyoshima, Koh-ei; Ishida, Kentaro; Ogawa, Miho; Sakakibara, Kei; Asakawa, Kyosuke; Kashiwakura, Akitoshi; Oshima, Masamitsu; Minamide, Ryohei; Sato, Akio; Yoshitake, Toshihiro; Takeda, Akira; Egusa, Hiroshi

    2016-01-01

    The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bio...

  10. The effect of wound dressings on a bio-engineered human dermo-epidermal skin substitute in a rat model

    OpenAIRE

    Hüging, Martina; Biedermann, Thomas; Sobrio, Monia; Meyer, Sarah; Böttcher-Haberzeth, Sophie; Manuel, Edith; Horst, Maya; Hynes, Sally; Reichmann, Ernst; Schiestl, Clemens; Hartmann-Fritsch, Fabienne

    2017-01-01

    Autologous bio-engineered dermo-epidermal skin substitutes are a promising treatment for large skin defects such as burns. For their successful clinical application, the graft dressing must protect and support the keratinocyte layer and, in many cases, possess antimicrobial properties. However, silver in many antimicrobial dressings may inhibit keratinocyte growth and differentiation. The purpose of our study is to evaluate the effect of various wound dressings on the healing of a human hydro...

  11. New course in bioengineering and bioinspired design.

    Science.gov (United States)

    Erickson, Jonathan C

    2012-01-01

    The past two years, a new interdisciplinary course has been offered at Washington and Lee University (Lexington, VA, USA), which seeks to surmount barriers that have traditionally existed between the physical and life sciences. The course explores the physiology leading to the physical mechanisms and engineering principles that endow the astonishing navigation abilities and sensory mechanisms of animal systems. The course also emphasizes how biological systems are inspiring novel engineering designs. Two (among many) examples are how the adhesion of the gecko foot inspired a new class of adhesives based on Van der Waals forces; and how the iridophore protein plates found in mimic octopus and squid act as tunable ¼ wave stacks, thus inspiring the engineering of optically tunable block copolymer gels for sensing temperature, pressure, or chemical gradients. A major component of this course is the integration of a 6-8 week long research project. To date, projects have included engineering: a soft-body robot whose motion mimics the inchworm; an electrical circuit to sense minute electric fields in aqueous environments based on the shark electrosensory system; and cyborg grasshoppers whose jump motion is controlled via an electronic-neural interface. Initial feedback has indicated that this course has served to increase student interaction and “cross-pollination” of ideas between the physical and life sciences. Student feedback also indicated a marked increase in desire and confidence to continue to pursue problems at the boundary of biology and engineering—bioengineering.

  12. Bioengineering in the oral cavity: our experience

    Directory of Open Access Journals (Sweden)

    Catalfamo L

    2013-10-01

    Full Text Available L Catalfamo,1 E Belli,2 C Nava,1 E Mici,1 A Calvo,1 B D'Alessandro,1 FS De Ponte1 1Unit of Maxillofacial Surgery, University of Messina, Azienda Ospedaliera Universitaria, Policlinico G Martino, Messina, Italy; 2Unit of Maxillofacial Surgery, University Rome Sapienza, Azienda Ospedaliera Sant Andrea, Rome, Italy Background: To date, there are no studies reported in the literature on the possible use of bovine collagen, oxidized regenerated cellulose, or synthetic hyaluronic acid medications in the oral cavity. The aim of this paper is to report the use of bovine collagen, oxidized regenerated cellulose, and synthetic hyaluronic acid medications to improve wound healing in the oral cavity by stimulating granulomatous tissue. Methods: From 2007 to 2011, 80 patients (median age 67 years suffering from oral mucosal lesions participated in this double-blind study. The patients were divided into two groups, each consisting of 40 patients. One group received conventional medications, while the other group of patients were treated with the advanced medications. Results: Advanced medications allowed re-epithelialization of the wound margin in 2–20 days, whereas patients receiving conventional medication showed a median healing duration of 45 days. Conclusion: The results of this study demonstrate that treating oral mucosal wounds with advanced medication has an advantage with regard to wound healing time, allowing patients to have a rapid, functional, and esthetic recovery. Keywords: bioengineering, oral cavity, mucosal recovery

  13. Case Study: Effects of a Partial-Debris Dam on Riverbank Erosion in the Parlung Tsangpo River, China

    Directory of Open Access Journals (Sweden)

    Clarence Edward Choi

    2018-02-01

    Full Text Available This paper examines two successive debris flows that deposited a total of 1.4 million m3 of sediment into the Parlung Tsangpo River in China in 2010. As a result of these deposits, a partial-debris dam was formed in the river. This dam rerouted the discharge in the river along one of the riverbanks, which supported a highway. The rerouted discharge eroded the riverbank and the highway eventually collapsed. To enhance our understanding of the threat posed by partial-debris dams, a field investigation was carried out to measure the discharge in the river and to collect soil samples of the collapsed riverbank. Findings from the field investigation were then used to back-analyze fluvial erosion along the riverbank using a combined erosion framework proposed in this study. This combined framework adopts a dam-breach erosion model which can capture the progressive nature of fluvial erosion by considering the particle size distribution of the soil being eroded. The results from the back-analysis were then evaluated against unique high-resolution images obtained from satellites. This case study not only highlights the consequences of the formation of partial-debris dams on nearby infrastructure, but it also proposes the use of a combined erosion framework to provide a first-order assessment of riverbank stability. Unique high-resolution satellite images are used to assess the proposed erosion framework and key challenges in assessing erosion are discussed.

  14. Bioengineered Proteins for Chemical/Biological Defense, Protection and Decontamination

    National Research Council Canada - National Science Library

    DeBoer, Kenneth

    2002-01-01

    Report developed under contract for STTR topic "ARMY01-T005". The purpose of the project was to demonstrate feasibility of developing transgenic chickens by methods invented by the Contractor and RI...

  15. Career development in Bioengineering/Biomedical Engineering: a student's roadmap.

    Science.gov (United States)

    Abu-Faraj, Ziad O

    2008-01-01

    Bioengineering/biomedical engineering education has progressed since the late 1950s and is still evolving in leading academic institutions worldwide. Today, Bioengineering/Biomedical Engineering is acclaimed as one of the most reputable fields within the global arena, and will likely be the catalyst for any future breakthroughs in Medicine and Biology. This paper provides a set of strategies and recommendations to be pursued by individuals aiming at planning and developing careers in this field. The paper targets the international student contemplating bioengineering/biomedical engineering as a career, with an underlying emphasis on the student within developing and transitional countries where career guidance is found deficient. The paper also provides a comprehensive definition of the field and an enumeration of its subdivisions.

  16. Glycan bioengineering in immunogen design for tumor T antigen immunotargeting

    DEFF Research Database (Denmark)

    Sendra, Victor G; Zlocowski, Natacha; Ditamo, Yanina

    2009-01-01

    MM2 energy function showed that pentalysine (Lys5) linker and benzyl (Bzl) residue enhance TFD rigidity of the glycosidic bond. Antibodies raised against BzlalphaTFD-Lys5 immunogen recognize tumor T antigen. Competitive assays confirm that TFD-related structures are the main glycan epitope...... to the bioengineered glycoconjugate inhibited CT26 tumor cell proliferation and reduced tumor growth in an in vivo mouse model. These results show that TFD bioengineering is a useful immunogenic strategy with potential application in cancer therapy. The same approach can be extended to other glycan immunogens......Bioengineering of Galbeta3GalNAcalpha, known as Thomsen-Friedenreich disaccharide (TFD), is studied to promote glycan immunogenicity and immunotargeting to tumor T antigen (Galbeta3GalNAcalpha-O-Ser/Thr). Theoretical studies on disaccharide conformations by energy minimization of structures using...

  17. Successful implantation of physiologically functional bioengineered mouse internal anal sphincter.

    Science.gov (United States)

    Raghavan, Shreya; Miyasaka, Eiichi A; Hashish, Mohamed; Somara, Sita; Gilmont, Robert R; Teitelbaum, Daniel H; Bitar, Khalil N

    2010-08-01

    We have previously developed bioengineered three-dimensional internal anal sphincter (IAS) rings from circular smooth muscle cells isolated from rabbit and human IAS. We provide proof of concept that bioengineered mouse IAS rings are neovascularized upon implantation into mice of the same strain and maintain concentric smooth muscle alignment, phenotype, and IAS functionality. Rings were bioengineered by using smooth muscle cells from the IAS of C57BL/6J mice. Bioengineered mouse IAS rings were implanted subcutaneously on the dorsum of C57BL/6J mice along with a microosmotic pump delivering fibroblast growth factor-2. The mice remained healthy during the period of implantation, showing no external signs of rejection. Mice were killed 28 days postsurgery and implanted IAS rings were harvested. IAS rings showed muscle attachment, neovascularization, healthy color, and no external signs of infection or inflammation. Assessment of force generation on harvested IAS rings showed the following: 1) spontaneous basal tone was generated in the absence of external stimulation; 2) basal tone was relaxed by vasoactive intestinal peptide, nitric oxide donor, and nifedipine; 3) acetylcholine and phorbol dibutyrate elicited rapid-rising, dose-dependent, sustained contractions repeatedly over 30 min without signs of muscle fatigue; and 4) magnitudes of potassium chloride-induced contractions were 100% of peak maximal agonist-induced contractions. Our preliminary results confirm the proof of concept that bioengineered rings are neovascularized upon implantation. Harvested rings maintain smooth muscle alignment and phenotype. Our physiological studies confirm that implanted rings maintain 1) overall IAS physiology and develop basal tone, 2) integrity of membrane ionic characteristics, and 3) integrity of membrane associated intracellular signaling transduction pathways for contraction and relaxation by responding to cholinergic, nitrergic, and VIP-ergic stimulation. IAS smooth muscle

  18. Soil bioengineering methods for abandoned mine land surface drainage channels

    Energy Technology Data Exchange (ETDEWEB)

    Sotir, R.B.; Simms, A.P.; Sweigard, R.J.; Hammer, P.; Graves, D.H.; Adkins, M. [Robbin B. Sotir & Associates, Marietta, GA (USA)

    1999-07-01

    Research to determine the suitability of soil bioengineering for slope stabilization at abandoned surface mining sites is described. The technology uses live woody plant material as a structural component, in this case live fascine with coir erosion control fabric made from coconut. A large water collection pond draining to nine channels on the slope below was constructed as a test site. The pond has drainage channels for testing at low, intermediate, and steep slope grades. Each group of three channels is composed of one riprap rock channel, one gabion channel, and one soil bioengineering channel. The channels will be tested summer 1999. 11 refs., 5 figs., 2 tabs., 8 photos.

  19. The future of starch bioengineering: GM microorganisms or GM plants?

    DEFF Research Database (Denmark)

    Hebelstrup, Kim; Sagnelli, Domenico; Blennow, Andreas

    2015-01-01

    , tubers and cereal grains to provide a GM crop as an alternative to the use of enzymes from GM microorganisms. We here discuss these techniques in relation to important structural features and modifications of starches such as: starch phosphorylation, starch hydrolysis, chain transfer/branching and novel...... concepts of hybrid starch-based polysaccharides. In planta starch bioengineering is generally challenged by yield penalties and inefficient production of the desired product. However, in some situations, GM crops for starch bioengineering without deleterious effects have been achieved....

  20. Study of water quality improvements during riverbank filtration at three midwestern United States drinking water utilities

    Science.gov (United States)

    Weiss, W.; Bouwer, E.; Ball, W.; O'Melia, C.; Lechevallier, M.; Arora, H.; Aboytes, R.; Speth, T.

    2003-04-01

    Riverbank filtration (RBF) is a process during which surface water is subjected to subsurface flow prior to extraction from wells. During infiltration and soil passage, surface water is subjected to a combination of physical, chemical, and biological processes such as filtration, dilution, sorption, and biodegradation that can significantly improve the raw water quality (Tufenkji et al, 2002; Kuehn and Mueller, 2000; Kivimaki et al, 1998; Stuyfzand, 1998). Transport through alluvial aquifers is associated with a number of water quality benefits, including removal of microbes, pesticides, total and dissolved organic carbon (TOC and DOC), nitrate, and other contaminants (Hiscock and Grischek, 2002; Tufenkji et al., 2002; Ray et al, 2002; Kuehn and Mueller, 2000; Doussan et al, 1997; Cosovic et al, 1996; Juttner, 1995; Miettinen et al, 1994). In comparison to most groundwater sources, alluvial aquifers that are hydraulically connected to rivers are typically easier to exploit (shallow) and more highly productive for drinking water supplies (Doussan et al, 1997). Increased applications of RBF are anticipated as drinking water utilities strive to meet increasingly stringent drinking water regulations, especially with regard to the provision of multiple barriers for protection against microbial pathogens, and with regard to tighter regulations for disinfection by-products (DBPs), such as trihalomethanes (THMs) and haloacetic acids (HAAs). In the above context, research was conducted to document the water quality benefits during RBF at three major river sources in the mid-western United States, specifically with regard to DBP precursor organic matter and microbial pathogens. Specific objectives were to: 1. Evaluate the merits of RBF for removing/controlling DBP precursors and certain other drinking water contaminants (e.g. microorganisms). 2. Evaluate whether RBF can improve finished drinking water quality by removing and/or altering natural organic matter (NOM) in a

  1. Successful Implantation of Bioengineered, Intrinsically Innervated, Human Internal Anal Sphincter

    Science.gov (United States)

    Raghavan, Shreya; Gilmont, Robert R.; Miyasaka, Eiichi A.; Somara, Sita; Srinivasan, Shanthi; Teitelbaum, Daniel H; Bitar, Khalil N.

    2011-01-01

    Background & Aims To restore fecal continence, the weakened pressure of the internal anal sphincter (IAS) must be increased. We bioengineered intrinsically innervated human IAS, to emulate sphincteric physiology, in vitro. Methods We co-cultured human IAS circular smooth muscle with immortomouse fetal enteric neurons. We investigated the ability of bioengineered innervated human IAS, implanted in RAG1−/− mice, to undergo neovascularization and preserve the physiology of the constituent myogenic and neuronal components. Results The implanted IAS was neovascularized in vivo; numerous blood vessels were observed with no signs of inflammation or infection. Real-time force acquisition from implanted and pre-implant IAS showed distinct characteristics of IAS physiology. Features included the development of spontaneous myogenic basal tone; relaxation of 100% of basal tone in response to inhibitory neurotransmitter vasoactive intestinal peptide (VIP) and direct electrical field stimulation of the intrinsic innervation; inhibition of nitrergic and VIPergic EFS-induced relaxation (by antagonizing nitric oxide synthesis or receptor interaction); contraction in response to cholinergic stimulation with acetylcholine; and intact electromechanical coupling (evidenced by direct response to potassium chloride). Implanted, intrinsically innervated bioengineered human IAS tissue preserved the integrity and physiology of myogenic and neuronal components. Conclusion Intrinsically innervated human IAS bioengineered tissue can be successfully implanted in mice. This approach might be used to treat patients with fecal incontinence. PMID:21463628

  2. European governance challenges in 21st century bio-engineering

    NARCIS (Netherlands)

    Est, van R.; Stemerding, D.; Est, van R.; Stemerding, D.

    2012-01-01

    This Final Report discussed four case studies (whole genome sequencing, neuromodulation, biocybernetic adaptation and standardization in synthetic biology) focusing on particular developments in the four fields of 21st-century bio-engineering that were explored more extensively in the earlier

  3. 76 FR 67178 - Riverbank Hydro No. 15 LLC; Notice of Preliminary Permit Application Accepted for Filing and...

    Science.gov (United States)

    2011-10-31

    ... Corps. Applicant Contact: Mr. Kuo-Bao Tong, Riverbank Power Corporation, Royal Bank Plaza, South Tower, P.O. Box 166, 200 Bay Street, Suite 3230, Toronto, ON, Canada M5J2J4. (416) 861-0092 x 154. FERC...

  4. 77 FR 10740 - Riverbank Hydro No. 22, LLC; FFP Project 93, LLC; Notice Announcing Preliminary Permit Drawing

    Science.gov (United States)

    2012-02-23

    ...] Riverbank Hydro No. 22, LLC; FFP Project 93, LLC; Notice Announcing Preliminary Permit Drawing The..., will conduct a random drawing to determine the filing priority of the applicants identified in this... regulations.\\2\\ The priority established by this drawing will be used to determine which applicant, among...

  5. 76 FR 67175 - Riverbank Hydro No. 2 LLC, Lock Hydro Friends Fund XXXVI, Arkansas Electric Cooperative Corp...

    Science.gov (United States)

    2011-10-31

    ...; 14149-000] Riverbank Hydro No. 2 LLC, Lock Hydro Friends Fund XXXVI, Arkansas Electric Cooperative Corp... Lock Hydro Friends Fund XXXVI (Lock Hydro) and on April 11, 2011, Arkansas Electric Cooperative Corp... & Dam No. 3, as directed by the Corps. Applicant Contact: Mr. Wayne F. Krouse, Hydro Green Energy, 5090...

  6. Assessing the fate of organic micropollutants during riverbank filtration utilizing field studies and laboratory test systems

    Science.gov (United States)

    Schmidt, C. K.; Lange, F. T.; Sacher, F.; Baus, C.; Brauch, H.-J.

    2003-04-01

    In Germany and other highly populated countries, several waterworks use riverbank filtration as a first step in the treatment of river water for water supplies. Unfortunately, industrial and municipal discharges and the influence of agriculture lead to the pollution of rivers and lakes by a number of organic chemicals. In order to assess the impact of those organic micropollutants on the quality of drinking water, it is necessary to clarify their fate during infiltration and underground passage. The fate of organic micropollutants in a river water-groundwater infiltration system is mainly determined by adsorption mechanisms and biological transformations. One possibility to simulate the microbial degradation of single compounds during riverbank filtration is the use of laboratory test filter systems, that are operated as biological fixed-bed reactors under aerobic conditions. The benefit and meaningfulness of those test filters was evaluated on the basis of selected target compounds by comparing the results derived from test filter experiments with field studies under environmental conditions at the River Rhine. Samples from the river and from groundwater of a well characterized aerobic infiltration pathway were analyzed over a time period of several years for a spectrum of organic micropollutants. Target compounds comprised several contaminants relevant for the aquatic environment, such as complexing agents, aromatic sulfonates, pharmaceuticals (including iodinated X ray contrast media), and MTBE. Furthermore, the behaviour of some target compounds during aerobic riverbank filtration was compared to their fate along a section of an anaerobic (oxygen-depleted) aquifer at the River Ruhr that is characterized by a transition state between sulfate reduction and methane production. While some organic micropollutants showed no major differences, the elimination of others turned out to be clearly dependent on the underlying redox processes in the groundwater. The

  7. Design Procedure Enhanced with Numerical Modeling to Mitigate River-Bank Erosion

    Directory of Open Access Journals (Sweden)

    Elhakeem Mohamed

    2016-01-01

    Full Text Available In this study, the 2D Finite Element Surface Water Modeling System (FESWMS is used to design barb structures to mitigate river bank erosion in a stream reach located on the Raccoon River near Adel, Iowa, USA just upstream of the US Highway Bridge 169. FESWMS is used also to access the barbs effect on the study reach. The model results showed that the proposed barb structures successfully reduced the flow velocity along the outside bank and increased the velocity in the center of the stream, thereby successfully increased the conveyance towards the core of the river. The estimated velocities values along the river-banks where the barbs exist were within the recommended values for channel stability design. Thus, the barb structures were able to reduce the erosion along the bankline.

  8. Identifying the effects of parameter uncertainty on the reliability of riverbank stability modelling

    Science.gov (United States)

    Samadi, A.; Amiri-Tokaldany, E.; Darby, S. E.

    2009-05-01

    Bank retreat is a key process in fluvial dynamics affecting a wide range of physical, ecological and socioeconomic issues in the fluvial environment. To predict the undesirable effects of bank retreat and to inform effective measures to prevent it, a wide range of bank stability models have been presented in the literature. These models typically express bank stability by defining a factor of safety as the ratio of driving and resisting forces acting on the incipient failure block. These forces are affected by a range of controlling factors that include such aspects as the bank profile (bank height and angle), the geotechnical properties of the bank materials, as well as the hydrological status of the riverbanks. In this paper we evaluate the extent to which uncertainties in the parameterization of these controlling factors feed through to influence the reliability of the resulting bank stability estimate. This is achieved by employing a simple model of riverbank stability with respect to planar failure (which is the most common type of bank stability model) in a series of sensitivity tests and Monte Carlo analyses to identify, for each model parameter, the range of values that induce significant changes in the simulated factor of safety. These identified parameter value ranges are compared to empirically derived parameter uncertainties to determine whether they are likely to confound the reliability of the resulting bank stability calculations. Our results show that parameter uncertainties are typically high enough that the likelihood of generating unreliable predictions is typically very high (> ˜ 80% for predictions requiring a precision of < ± 15%). Because parameter uncertainties are derived primarily from the natural variability of the parameters, rather than measurement errors, much more careful attention should be paid to field sampling strategies, such that the parameter uncertainties and consequent prediction unreliabilities can be quantified more

  9. Preliminary investigation of the transport of small plastic litter along a vegetated riverbank

    Science.gov (United States)

    Liu, Da; Valyrakis, Manousos

    2017-04-01

    Plastics are widely used in consumer products, due to its low cost, low weight and high durability compared to other types of materials. Contamination of marine ecosystems due to plastics (including microplastics) is a challenge that has received a lot of attention due to the significant risks it poses for the environment and human health. Plastics find their way to the ocean from land via the river system. Studying and obtaining a better understanding of the mechanisms contributing to the fate of plastic litter is therefore important in proactively devising methods to reduce their quantity or produce designs to trap plastic pollutants and prevent them from entering the ocean through estuaries. In this context, it is a common observation of hydraulic practitioners and field geomorphologists, that plastic litter can be trapped within riparian vegetation patches along streams or canals, which can be washed away in periods of high flows. To this goal this study aims to use a series of purpose specific physical experiments to examine the mechanisms of dispersion of plastic litter along the water surface of a channel with simulated riparian vegetation. The set of experiments are conducted in a recirculating flume with rigid riverbank and riparian vegetation modeled by a large number of acrylic rods, placed on the top of the riverbank section. Six different sizes of pieces of Styrofoam are used to simulate plastic litter. These are released from different locations upstream and in the vicinity of the riparian vegetation for various configurations (linear, staggered and random) of characteristic solid density. The trajectory of the plastic litter is recorded with a camera offering a top view of the arrangement. From the analysis of this a variety of results are obtained including transport metrics (including transport velocity and time to trapping) and litter-trapping location. The relation between the size of the litter, the vegetation configuration and the traveling

  10. Biomimetic chemical sensors using bioengineered olfactory and taste cells.

    Science.gov (United States)

    Du, Liping; Zou, Ling; Zhao, Luhang; Wang, Ping; Wu, Chunsheng

    2014-01-01

    Biological olfactory and taste systems are natural chemical sensing systems with unique performances for the detection of environmental chemical signals. With the advances in olfactory and taste transduction mechanisms, biomimetic chemical sensors have achieved significant progress due to their promising prospects and potential applications. Biomimetic chemical sensors exploit the unique capability of biological functional components for chemical sensing, which are often sourced from sensing units of biological olfactory or taste systems at the tissue level, cellular level, or molecular level. Specifically, at the cellular level, there are mainly two categories of cells have been employed for the development of biomimetic chemical sensors, which are natural cells and bioengineered cells, respectively. Natural cells are directly isolated from biological olfactory and taste systems, which are convenient to achieve. However, natural cells often suffer from the undefined sensing properties and limited amount of identical cells. On the other hand, bioengineered cells have shown decisive advantages to be applied in the development of biomimetic chemical sensors due to the powerful biotechnology for the reconstruction of the cell sensing properties. Here, we briefly summarized the most recent advances of biomimetic chemical sensors using bioengineered olfactory and taste cells. The development challenges and future trends are discussed as well.

  11. Inherent risks associated with manufacture of bioengineered ocular surface tissue.

    Science.gov (United States)

    Schwab, Ivan R; Johnson, Nigel T; Harkin, Damien G

    2006-12-01

    To review the potential health risks associated with bioengineered ocular surface tissue, which serves as a bellwether for other tissues. All clinical trials using bioengineered ocular surface tissue published between July 1, 1996, and June 30, 2005, were reviewed with respect to materials used and statements of risk assessment, risk remediation, adverse events, manufacturing standards, and regulatory oversight. Ninety-five percent of investigational protocols used 1 or more animal-derived products and an overlapping 95% used 1 or more donor human tissues. Consideration of risks reveals a very low probability of potential harm but a significant risk of disability or death if such an event were to occur. Details of ethics approval, patient consent, and donor serologic test results were not consistently provided. No references were made to risk assessment or to codes of manufacturing and clinical practice. While a degree of risk is associated with bioengineered ocular surface tissue, investigational reports of this new technology have yet to address issues of risk management and regulatory oversight. Attention to risk and codes of manufacturing and clinical practice will be required for advancement of the technology. We suggest the adoption of international standards to address these issues.

  12. Tidal and Seasonal River Stage Fluctuations Impact the Formation of Permeable Natural Reactive Barriers in Riverbank Sediments

    Science.gov (United States)

    Shuai, P.; Myers, K.; Knappett, P.; Cardenas, M. B.

    2017-12-01

    River stage fluctuations, induced by ocean tides and rainfall, enhance the exchange between oxic river water and reducing groundwater. When mixing occurs within riverbank aquifers high in dissolved iron (Fe) and arsenic (As), the timing and extent of mixing likely control the accumulation and mobility of arsenic (As) within the hyporheic zone. Here we analyzed the impact of tidal and seasonal water level fluctuations on the formation of a Permeable Natural Reactive Barrier (PNRB) within an aquifer adjacent to the Meghna River, Bangladesh and its impact on As mobility. We found that the periodicity and amplitude of river stage fluctuations strongly control the spatial and temporal distribution of the PNRB, comprised of rapidly precipitated iron oxides, in this riverbank along a relatively straight reach of the Meghna River. The PNRB forms much faster and with higher concentration of Fe-oxide under semi-diurnal (12 hr) tidal fluctuations compared to simulations run assuming only neap-spring tides (14 day). As tidal amplitude increases, a larger contact area between oxic river water and reducing groundwater results which in turn leads to the horizontal expansion of the PNRB into the riverbank. Seasonal fluctuations expand the PNRB up to 60 m horizontally and 5 m vertically. In contrast neap-spring tidal fluctuations result in a smaller PNRB that is 10 and 3 m in the horizontal and vertical dimensions. The predicted changes in the spatial distribution of iron oxides within the riverbank would trap and release As at different times of the year. The PNRB could act as a secondary source of As to drinking water aquifers under sustained groundwater pumping scenarios near the river.

  13. Bioengineering the Uterus: An Overview of Recent Advances and Future Perspectives in Reproductive Medicine.

    Science.gov (United States)

    Campo, Hannes; Cervelló, Irene; Simón, Carlos

    2017-07-01

    Since the initial in vitro attempts to more complex models, research on uterine regeneration is moving towards the creation of a functional bioengineered uterus with possible clinical applications. We describe here the most relevant advances in bioengineering of the uterus published in the last decades considering the use of stem cells and biomaterials as well as future developing techniques in Regenerative Medicine.

  14. Should bioengineering graduates seek employment in the defense industry?

    Science.gov (United States)

    Johnson, Arthur T

    2014-01-01

    They say that the difference between a mechanical engineer and a civil engineer is that the mechanical engineer develops weapons whereas a civil engineer designs targets. The implication is that some engineers are involved with building peaceful infrastructure whereas others contribute to destruction. This brings to mind the question: what is the proper role for engineers in the creation of weapons and defenses against them? In particular, should engineers specializing in biology or medicine be involved in the defense industry? After all, bioengineers are supposed to be builders or healers rather than warriors or destroyers.

  15. EDITORIAL: Bioengineering nanotechnology: towards the clinic Bioengineering nanotechnology: towards the clinic

    Science.gov (United States)

    Zhao, Weian; Karp, Jeffrey M.; Ferrari, Mauro; Serda, Rita

    2011-12-01

    special issue highlights some of the most recent advances in the exciting field of bioengineering nanotechnology. The themes covered in this issue include nanoparticle systems for tracking transplanted stem cells using a variety of imaging modalities, synthesis of smart nanomaterials for drug delivery and targeting specific intracellular organalles, elucidating how physical cues provided by nano- or micro-fabricated scaffolds impact cell phenotype, novel nanocomposite materials for biosensing, biomimetic assembly of nanostructures for studying molecular interactions, and high content analysis for assessing nanotoxicity. We believe next-generation nanomaterials that are currently under development will transform our understanding of biological mechanisms, and revolutionize clinical practice through ushering in new diagnostics and therapeutic paradigms. As nanotechnology becomes increasingly accessible to research laboratories, significant advances leading to paradigm shifts in basic biology and medicine will require connecting the right technology to the right problem and ensuring that the most pertinent criteria are correctly identified and addressed. References [1] Peer D et al Nat. Nanotechnol. 2 751-60 [2] Wang A Z, Langer R S and Farokhzad O C 2011 Annu. Rev. Med. doi: 10.1146/annurev-med-040210-162544 [3] Farokhzad O C and Langer R 2006 Adv. Drug Deliv. Rev. 58 1456-9 [4] Zhao W and Karp J M 2009 Nat. Mater. 8 453-4 [5] Ferreira L, Karp J M, Nobre L and Langer R 2008 Cell Stem Cell 3 136-46 [6] Zhao W and Karp J M 2009 ChemBioChem 10 2308-10 [7] Zhao W et al 2011 Nat. Nanotechnol. 6 524-31 [8] Stephan M T, Moon J J, Um S H, Bershteyn A and Irvine D J 2010 Nat. Med. 16 1035-41 [9] Sarkar D, Ankrum J A, Teo G S, Carman C V and Karp J M 2011 Biomaterials 32 3053-61

  16. Removal of Escherichia coli via low frequency electromagnetic field in riverbank filtration system.

    Science.gov (United States)

    Selamat, Rossitah; Abustan, Ismail; Rizal Arshad, Mohd; Mokhtar Kamal, Nurul Hana

    2018-04-01

    The removal of Escherichia coli (E. coli) via low frequency of electromagnetic field (LF-EMF) with different magnetic field was studied. LF-EMF is known as a high magnetic susceptibility method, which could affect E. coli growth without the usage of chemicals. The aim of this study was to investigate the removal of E. coli by using LF-EMF in water abstraction for the riverbank filtration (RBF) application. The effect of LF-EMF with the intensity of 2 to 10mT and 50Hz on coiled column of 1mm copper wire at 1 to 6 hours was assessed. The removal of E. coli after exposing to LF-EMF on the column model was measured using most probable number (MPN/100mL) and colonies forming unit (CFU/100mL) methods. Water flows into the column were varied up to 6 hours and with flowrate of 100 mL/min. Experimental results demonstrate that 100% of E. coli was removed at 8mT after 6 hours exposure. The magnetic field at 10mT removed 100% of E. coli after 4 hours exposure. The results obtained in this study proved that the LF-EMF was efficient in E. coli removal from RBF system. These finding indicated that the LF-EMF intensities and time of exposure can affect the removal of E. coli.

  17. Nitrogen and Organics Removal during Riverbank Filtration along a Reclaimed Water Restored River in Beijing, China

    Directory of Open Access Journals (Sweden)

    Weiyan Pan

    2018-04-01

    Full Text Available Reclaimed water has been widely used to restore rivers and lakes in water scarce areas as well as in Beijing municipality, China. However, refilling the rivers with reclaimed water may result in groundwater pollution. A three-year field monitoring program was conducted to assess the effect of a riverbank filtration (RBF system on the removal of nitrogen and organics from the Qingyang River of Beijing, which is replenished with reclaimed water. Water samples from the river, sediment, and groundwater were collected for NO3-N, NH4-N, and chemical oxygen demand (COD was measured. The results indicate that about 85% of NO3-N was removed from the riverbed sediments. Approximate 92% of NH4-N was removed during the infiltration of water from river to aquifer. On average, 54% of COD was removed by RBF. The attenuation of NO3-N through RBF to the groundwater varied among seasons and was strongly related to water temperature. On the other hand, no obvious temporal variability was identified in the removal of COD. These results suggest that the RBF system is an effective barrier against NO3-N, NH4-N and COD in the Qingyang River, as well as those rivers with similar geological and climatic conditions refilled with reclaimed water.

  18. Application of risk-based assessment and management to riverbank filtration sites in India.

    Science.gov (United States)

    Bartak, Rico; Page, Declan; Sandhu, Cornelius; Grischek, Thomas; Saini, Bharti; Mehrotra, Indu; Jain, Chakresh K; Ghosh, Narayan C

    2015-03-01

    This is the first reported study of a riverbank filtration (RBF) scheme to be assessed following the Australian Guidelines for Managed Aquifer Recharge. A comprehensive staged approach to assess the risks from 12 hazards to human health and the environment has been undertaken. Highest risks from untreated ground and Ganga River water were identified with pathogens, turbidity, iron, manganese, total dissolved solids and total hardness. Recovered water meets the guideline values for inorganic chemicals and salinity but exceeds limits for thermotolerant coliforms frequently. A quantitative microbial risk assessment undertaken on the water recovered from the aquifer indicated that the residual risks of 0.00165 disability-adjusted life years (DALYs) posed by the reference bacteria Escherichia coli O157:H7 were below the national diarrhoeal incidence of 0.027 DALYs and meet the health target in this study of 0.005 DALYs per person per year, which corresponds to the World Health Organization (WHO) regional diarrhoeal incidence in South-East Asia. Monsoon season was a major contributor to the calculated burden of disease and final DALYs were strongly dependent on RBF and disinfection pathogen removal capabilities. Finally, a water safety plan was developed with potential risk management procedures to minimize residual risks related to pathogens.

  19. Bioengineering Spin-Offs from Dynamical Systems Theory

    Science.gov (United States)

    Collins, J. J.

    1997-03-01

    Recently, there has been considerable interest in applying concepts and techniques from dynamical systems and statistical physics to physiological systems. In this talk, we present work dealing which two active topics in this area: stochastic resonance and (2) chaos control. Stochastic resonance is a phenomenon wherein the response of nonlinear system to a weak input signal is optimally enhanced by the presence of a particular level of noise. Here we demonstrate that noise-based techniques can be used to lower sensory detection thresholds in humans. We discuss how from a bioengineering and clinical standpoint, these developments may be particularly relevant for individuals with elevated sensory thresholds, such as older adults and patients with peripheral neuropathy. Chaos control techniques have been applied to a wide range of experimental systems, including biological preparations. The application of chaos control to biological systems has led to speculations that these methods may be clinically useful. Here we demonstrate that the principles of chaos control can be utilized to stabilize underlying unstable periodic orbits in non-chaotic biological systems. We discuss how from a bioengineering and clinical standpoint, these developments may be important for suppressing or eliminating certain types of cardiac arrhythmias.

  20. The future of starch bioengineering: GM microorganisms or GM plants?

    Directory of Open Access Journals (Sweden)

    Kim Henrik eHebelstrup

    2015-04-01

    Full Text Available Plant starches regularly require extensive modification to permit subsequent applications. Such processing is usually done by the use of chemical and/or physical treatments. The use of recombinant enzymes produced by large-scale fermentation of GM microorganisms is increasingly used in starch processing and modification, sometimes as an alternative to chemical or physical treatments. However, as a means to impart the modifications as early as possible in the starch production chain, similar recombinant enzymes may also be expressed in planta in the developing starch storage organ such as in roots, tubers and cereal grains to provide a GM crop as an alternative to the use of enzymes from GM microorganisms. We here discuss these techniques in relation to important structural features and modifications of starches such as: starch phosphorylation, starch hydrolysis, chain transfer/branching and novel concepts of hybrid starch-based polysaccharides. In planta starch bioengineering is generally challenged by yield penalties and inefficient production of the desired product. However in some situations, GM crops for starch bioengineering without deleterious effects have been achieved.

  1. Bioengineering natural product biosynthetic pathways for therapeutic applications.

    Science.gov (United States)

    Wu, Ming-Cheng; Law, Brian; Wilkinson, Barrie; Micklefield, Jason

    2012-12-01

    With the advent of next-generation DNA sequencing technologies, the number of microbial genome sequences has increased dramatically, revealing a vast array of new biosynthetic gene clusters. Genomics data provide a tremendous opportunity to discover new natural products, and also to guide the bioengineering of new and existing natural product scaffolds for therapeutic applications. Notably, it is apparent that the vast majority of biosynthetic gene clusters are either silent or produce very low quantities of the corresponding natural products. It is imperative therefore to devise methods for activating unproductive biosynthetic pathways to provide the quantities of natural products needed for further development. Moreover, on the basis of our expanding mechanistic and structural knowledge of biosynthetic assembly-line enzymes, new strategies for re-programming biosynthetic pathways have emerged, resulting in focused libraries of modified products with potentially improved biological properties. In this review we will focus on the latest bioengineering approaches that have been utilised to optimise yields and increase the structural diversity of natural product scaffolds for future clinical applications. Copyright © 2012 Elsevier Ltd. All rights reserved.

  2. Processing Techniques and Applications of Silk Hydrogels in Bioengineering

    Directory of Open Access Journals (Sweden)

    Michael Floren

    2016-09-01

    Full Text Available Hydrogels are an attractive class of tunable material platforms that, combined with their structural and functional likeness to biological environments, have a diversity of applications in bioengineering. Several polymers, natural and synthetic, can be used, the material selection being based on the required functional characteristics of the prepared hydrogels. Silk fibroin (SF is an attractive natural polymer for its excellent processability, biocompatibility, controlled degradation, mechanical properties and tunable formats and a good candidate for the fabrication of hydrogels. Tremendous effort has been made to control the structural and functional characteristic of silk hydrogels, integrating novel biological features with advanced processing techniques, to develop the next generation of functional SF hydrogels. Here, we review the several processing methods developed to prepare advanced SF hydrogel formats, emphasizing a bottom-up approach beginning with critical structural characteristics of silk proteins and their behavior under specific gelation environments. Additionally, the preparation of SF hydrogel blends and other advanced formats will also be discussed. We conclude with a brief description of the attractive utility of SF hydrogels in relevant bioengineering applications.

  3. Model for determining the load or carrying capacity of rivers and riverbanks for recreational use

    Directory of Open Access Journals (Sweden)

    Otero, I.

    2015-12-01

    Full Text Available The aim of this article is to determine the load or functional carrying capacity for the assimilation and recovery of rivers and riverbanks by compiling information on the environment, and to assess the potential for recreation from the environmental and scenic point of view. We set out to find the most significant basis for analysing the pressures limiting carrying capacity and constraining the demand for recreational use. This methodology will help planners and legislators in their decision-making. We also propose a methodology for assessing the recreational use of riverbanks, tested in a number of sites in the Madrid Region, in terms of the satisfaction the activity produces in the users, and the load or carrying capacity of the surrounding environment. A pilot site assessment is presented as a validation of the proposed methodology.El objetivo de este artículo es, determinar la capacidad de carga o acogida funcional, de asimilación y de recuperación de los márgenes y los cursos fluviales mediante la recopilación de información del medio; el potencial recreativo se evalúa además desde un doble punto de vista: ecológico y paisajístico. La forma en que se enfoca el problema y se presenta la metodología y los resultados puede resultar de gran utilidad para planificadores y legisladores. La metodología propuesta para valorar el uso recreativo de los márgenes fluviales, se aplica a una serie de parcelas en la Comunidad de Madrid, teniendo en cuenta la satisfacción que la actividad produce en los usuarios, y la capacidad de carga o acogida del entorno en el que se desarrollan. Como resultado, se presenta la valoración de parcelas piloto, que servirían para validar la metodología propuesta. [fr] Le but de cet article est, déterminer la capacité de charge ou réception fonctionnelle, l’assimilation et rétablissement des marges et les cours de la rivière par le biais de la collecte d’informations de l’environnement: le

  4. Wave-current induced erosion of cohesive riverbanks in northern Manitoba, Canada

    Science.gov (United States)

    Kimiaghalam, N.; Clark, S.; Ahmari, H.; Hunt, J.

    2015-03-01

    The field of cohesive soil erosion is still not fully understood, in large part due to the many soil parameters that affect cohesive soil erodibility. This study is focused on two channels, 2-Mile and 8-Mile channels in northern Manitoba, Canada, that were built to connect Lake Winnipeg with Playgreen Lake and Playgreen Lake with Kiskikittogisu Lake, respectively. The banks of the channels consist of clay rich soils and alluvial deposits of layered clay, silts and sands. The study of erosion at the sites is further complicated because the flow-induced erosion is combined with the effects of significant wave action due to the large fetch length on the adjacent lakes, particularly Lake Winnipeg that is the seventh largest lake in North America. The study included three main components: field measurements, laboratory experiments and numerical modelling. Field measurements consisted of soil sampling from the banks and bed of the channels, current measurements and water sampling. Grab soil samples were used to measure the essential physical and electrochemical properties of the riverbanks, and standard ASTM Shelby tube samples were used to estimate the critical shear stress and erodibility of the soil samples using an erosion measurement device (EMD). Water samples were taken to estimate the sediment concentration profile and also to monitor changes in sediment concentration along the channels over time. An Acoustic Doppler Current Profiler (ADCP) was used to collect bathymetry and current data, and two water level gauges have been installed to record water levels at the entrance and outlet of the channels. The MIKE 21 NSW model was used to simulate waves using historical winds and measured bathymetry of the channels and lakes. Finally, results from the wave numerical model, laboratory tests and current measurement were used to estimate the effect of each component on erodibility of the cohesive banks.

  5. Smart maintenance of riverbanks using a standard data layer and Augmented Reality

    Science.gov (United States)

    Pierdicca, Roberto; Frontoni, Emanuele; Zingaretti, Primo; Mancini, Adriano; Malinverni, Eva Savina; Tassetti, Anna Nora; Marcheggiani, Ernesto; Galli, Andrea

    2016-10-01

    Linear buffer strips (BS) along watercourses are commonly adopted to reduce run-off, accumulation of bank-top sediments and the leaking of pesticides into fresh-waters, which strongly increase water pollution. However, the monitoring of their conditions is a difficult task because they are scattered over wide rural areas. This work demonstrates the benefits of using a standard data layer and Augmented Reality (AR) in watershed control and outlines the guideline of a novel approach for the health-check of linear BS. We designed a mobile environmental monitoring system for smart maintenance of riverbanks by embedding the AR technology within a Geographical Information System (GIS). From the technological point of view, the system's architecture consists of a cloud-based service for data sharing, using a standard data layer, and of a mobile device provided with a GPS based AR engine for augmented data visualization. The proposed solution aims to ease the overall inspection process by reducing the time required to run a survey. Indeed, ordinary operational survey conditions are usually performed basing the fieldwork on just classical digitized maps. Our application proposes to enrich inspections by superimposing information on the device screen with the same point of view of the camera, providing an intuitive visualization of buffer strip location. This way, the inspection officer can quickly and dynamically access relevant information overlaying geographic features, comments and other contents in real time. The solution has been tested in fieldwork to prove at what extent this cutting-edge technology contributes to an effective monitoring over large territorial settings. The aim is to encourage officers, land managers and practitioners toward more effective monitoring and management practices.

  6. Hydraulic and hydrogeochemical characteristics of a riverbank filtration site in rural India.

    Science.gov (United States)

    Boving, T B; Choudri, B S; Cady, P; Cording, A; Patil, K; Reddy, Veerabaswant

    2014-07-01

    A riverbank filtration (RBF) system was tested along the Kali River in rural part of the state of Karnataka in India. The polluted river and water from open wells served the local population as their principal irrigation water resource and some used it for drinking. Four RBF wells (up to 25 m deep) were installed. The mean hydraulic conductivity of the well field is 6.3 x 10(-3) cm/s and, based on Darcy's law, the water travel time from the river to the principal RBF well (MW3) is 45.2 days. A mixing model based on dissolved silica concentrations indicated that, depending on the distance from the river and closeness to irrigated rice fields, approximately 27 to 73% of the well water originated from groundwater. Stable isotopic data indicates that a fraction of the water was drawn in from the nearby rice fields that were irrigated with river water. Relative to preexisting drinking water sources (Kali River and an open well), RBF well water showed lower concentration of dissolved metals (60.1% zinc, 27.8% cadmium, 83.9% lead, 75.5% copper, 100% chromium). This study demonstrates that RBF technology can produce high-quality water from low-quality surface water sources in a rural, tropical setting typical for many emerging economies. Further, in parts of the world where flood irrigation is common, RBF well water may draw in infiltrated irrigation water, which possibly alters its geochemical composition. A combination of more than one mixing model, silica together with stable isotopes, was shown to be useful explaining the origin of the RBF water at this study site.

  7. Rhizobia symbiosis of seven leguminous species growing along Xindian riverbank of Northern Taiwan

    Directory of Open Access Journals (Sweden)

    Cheng-Tai Huang

    2018-01-01

    Full Text Available Legume-rhizobia symbioses of seven leguminous species growing along Xindian riverbank of Northern Taiwan were investigated in this study. These legumes form either determinate or indeterminate types of root nodules. The determinate nodules of Alysicarpus vaginalis, Desmodium. triflorum, D. heterophyllum, Sesbania cannabina and the indeterminate nodules of Mimosa pudica harbored bacteroids of morphological uniformity (length of 1-3 μm, while the indeterminate nodules of Crotalaria zanzibarica and Trifolium repens contained bacteroids of highly pleomorphism (size varying from 1 to 5 μm. The enclosed bacteria were isolated from respective nodules, and twenty slow-growing and nine fast-growing rhizobial isolates were recovered. The slow-growing isolates were classified to the genus Bradyrhizobium based on the 16S rRNA sequences, whereas the fast-growing rhizobia comprise four genera, Neorhizobium, Rhizobium, Cupriavidus and Paraburkholderia. Results of stable isotope analyses revealed that the seven leguminous species had similar and consistently negative δ15N values in leaves (mean of -1.2 ‰, whereas the values were positive (varying from 3.7 to 7.3 ‰ in the nodules. These values were significantly higher in the indeterminate nodules than those in the determinate ones. In addition, variations in the values of leaf δ13C (varying from -29 to -34‰ among the seven legumes were measured, indicating their photosynthetic water use efficiencies were different. This is the first field survey to report the rhizobial diversity and the nutrient relationships of sympatric legume in Taiwan.

  8. Wave-current induced erosion of cohesive riverbanks in northern Manitoba, Canada

    Directory of Open Access Journals (Sweden)

    N. Kimiaghalam

    2015-03-01

    Full Text Available The field of cohesive soil erosion is still not fully understood, in large part due to the many soil parameters that affect cohesive soil erodibility. This study is focused on two channels, 2-Mile and 8-Mile channels in northern Manitoba, Canada, that were built to connect Lake Winnipeg with Playgreen Lake and Playgreen Lake with Kiskikittogisu Lake, respectively. The banks of the channels consist of clay rich soils and alluvial deposits of layered clay, silts and sands. The study of erosion at the sites is further complicated because the flow-induced erosion is combined with the effects of significant wave action due to the large fetch length on the adjacent lakes, particularly Lake Winnipeg that is the seventh largest lake in North America. The study included three main components: field measurements, laboratory experiments and numerical modelling. Field measurements consisted of soil sampling from the banks and bed of the channels, current measurements and water sampling. Grab soil samples were used to measure the essential physical and electrochemical properties of the riverbanks, and standard ASTM Shelby tube samples were used to estimate the critical shear stress and erodibility of the soil samples using an erosion measurement device (EMD. Water samples were taken to estimate the sediment concentration profile and also to monitor changes in sediment concentration along the channels over time. An Acoustic Doppler Current Profiler (ADCP was used to collect bathymetry and current data, and two water level gauges have been installed to record water levels at the entrance and outlet of the channels. The MIKE 21 NSW model was used to simulate waves using historical winds and measured bathymetry of the channels and lakes. Finally, results from the wave numerical model, laboratory tests and current measurement were used to estimate the effect of each component on erodibility of the cohesive banks.

  9. Ex Vivo Growth of Bioengineered Ligaments and Other Tissues

    Science.gov (United States)

    Altman, Gregory; Kaplan, David L.; Martin, Ivan; Vunjak-Novakovic, Gordana

    2005-01-01

    A method of growing bioengineered tissues for use in surgical replacement of damaged anterior cruciate ligaments has been invented. An anterior cruciate ligament is one of two ligaments (the other being the posterior cruciate ligament) that cross in the middle of a knee joint and act to prevent the bones in the knee from sliding forward and backward relative to each other. Anterior cruciate ligaments are frequently torn in sports injuries and traffic accidents, resulting in pain and severe limitations on mobility. By making it possible to grow replacement anterior cruciate ligaments that structurally and functionally resemble natural ones more closely than do totally synthetic replacements, the method could create new opportunities for full or nearly full restoration of functionality in injured knees. The method is also adaptable to the growth of bioengineered replacements for other ligaments (e.g., other knee ligaments as well as those in the hands, wrists, and elbows) and to the production of tissues other than ligaments, including cartilage, bones, muscles, and blood vessels. The method is based on the finding that the histomorphological properties of a bioengineered tissue grown in vitro from pluripotent cells within a matrix are affected by the direct application of mechanical force to the matrix during growth generation. This finding provides important new insights into the relationships among mechanical stress, biochemical and cell-immobilization methods, and cell differentiation, and is applicable to the production of the variety of tissues mentioned above. Moreover, this finding can be generalized to nonmechanical (e.g., chemical and electromagnetic) stimuli that are experienced in vivo by tissues of interest and, hence, the method can be modified to incorporate such stimuli in the ex vivo growth of replacements for the various tissues mentioned above. In this method, a three-dimensional matrix made of a suitable material is seeded with pluripotent stem

  10. Development of a statistical model for the determination of the probability of riverbank erosion in a Meditteranean river basin

    Science.gov (United States)

    Varouchakis, Emmanouil; Kourgialas, Nektarios; Karatzas, George; Giannakis, Georgios; Lilli, Maria; Nikolaidis, Nikolaos

    2014-05-01

    Riverbank erosion affects the river morphology and the local habitat and results in riparian land loss, damage to property and infrastructures, ultimately weakening flood defences. An important issue concerning riverbank erosion is the identification of the areas vulnerable to erosion, as it allows for predicting changes and assists with stream management and restoration. One way to predict the vulnerable to erosion areas is to determine the erosion probability by identifying the underlying relations between riverbank erosion and the geomorphological and/or hydrological variables that prevent or stimulate erosion. A statistical model for evaluating the probability of erosion based on a series of independent local variables and by using logistic regression is developed in this work. The main variables affecting erosion are vegetation index (stability), the presence or absence of meanders, bank material (classification), stream power, bank height, river bank slope, riverbed slope, cross section width and water velocities (Luppi et al. 2009). In statistics, logistic regression is a type of regression analysis used for predicting the outcome of a categorical dependent variable, e.g. binary response, based on one or more predictor variables (continuous or categorical). The probabilities of the possible outcomes are modelled as a function of independent variables using a logistic function. Logistic regression measures the relationship between a categorical dependent variable and, usually, one or several continuous independent variables by converting the dependent variable to probability scores. Then, a logistic regression is formed, which predicts success or failure of a given binary variable (e.g. 1 = "presence of erosion" and 0 = "no erosion") for any value of the independent variables. The regression coefficients are estimated by using maximum likelihood estimation. The erosion occurrence probability can be calculated in conjunction with the model deviance regarding

  11. Chloroplast bioengineering: the greening of chloroplasts in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Rebeiz, C.A.; Daniell, H.; Mattheis, J.R.

    1982-01-01

    During the first symposium on biotechnology in energy production and conservation we discussed the concept of cell-free agriculture and described its initial implementation via the assembly of an experimental photosynthetic reactor. Transducing photosynthetic membranes capable of converting solar energy, CO/sub 2/, and H/sub 2/O into chemical energy at extremely high efficiencies, approaching the theoretical efficiency of photosynthesis, are very essential components of the reactor. Although such membranes are not presently available, the technology for the bioengineering of such manmade membranes is developing very rapidly. We have been working on this problem for the past several years and as a first step in the right direction, it is now possible to biosynthesize chlorophyll in cell-free systems at rates higher than those observed in intact greening plants. This progress has been made possible by considerable improvements in analytical techniques and instrumentation and in our understanding of the biochemistry of the greening process. 4 figures, 6 tables.

  12. Dynamic, nondestructive imaging of a bioengineered vascular graft endothelium.

    Directory of Open Access Journals (Sweden)

    Bryce M Whited

    Full Text Available Bioengineering of vascular grafts holds great potential to address the shortcomings associated with autologous and conventional synthetic vascular grafts used for small diameter grafting procedures. Lumen endothelialization of bioengineered vascular grafts is essential to provide an antithrombogenic graft surface to ensure long-term patency after implantation. Conventional methods used to assess endothelialization in vitro typically involve periodic harvesting of the graft for histological sectioning and staining of the lumen. Endpoint testing methods such as these are effective but do not provide real-time information of endothelial cells in their intact microenvironment, rather only a single time point measurement of endothelium development. Therefore, nondestructive methods are needed to provide dynamic information of graft endothelialization and endothelium maturation in vitro. To address this need, we have developed a nondestructive fiber optic based (FOB imaging method that is capable of dynamic assessment of graft endothelialization without disturbing the graft housed in a bioreactor. In this study we demonstrate the capability of the FOB imaging method to quantify electrospun vascular graft endothelialization, EC detachment, and apoptosis in a nondestructive manner. The electrospun scaffold fiber diameter of the graft lumen was systematically varied and the FOB imaging system was used to noninvasively quantify the affect of topography on graft endothelialization over a 7-day period. Additionally, results demonstrated that the FOB imaging method had a greater imaging penetration depth than that of two-photon microscopy. This imaging method is a powerful tool to optimize vascular grafts and bioreactor conditions in vitro, and can be further adapted to monitor endothelium maturation and response to fluid flow bioreactor preconditioning.

  13. Improving Bioengineering Student Leadership Identity Via Training and Practice within the Core-Course.

    Science.gov (United States)

    Rosch, David M; Imoukhuede, P I

    2016-12-01

    The development of a leadership identity has become significant in bioengineering education as a result of an increasing emphasis on teamwork within the profession and corresponding shifts in accreditation criteria. Unsurprisingly, placing bioengineering students in teams to complete classroom-based projects has become a dominant pedagogical tool. However, recent research indicates that engineering students may not develop a leadership identity, much less increased leadership capacity, as a result of such efforts. Within this study, we assessed two similar sections of an introductory course in bioengineering; each placed students in teams, while one also included leadership training and leadership practice. Results suggest that students in the leadership intervention section developed a strong self-image of themselves as leaders compared to students in the control section. These data suggest that creating mechanisms for bioengineering students to be trained in leadership and to practice leadership behaviors within a classroom team may be keys for unlocking leadership development.

  14. Project Alexander the Great: a study on the world proliferation of bioengineering/biomedical engineering education.

    Science.gov (United States)

    Abu-Faraj, Ziad O

    2008-01-01

    Bioengineering/Biomedical Engineering is considered amongst the most reputable fields within the global arena, and will likely be the primer for any future breakthroughs in Medicine and Biology. Bioengineering/biomedical engineering education has evolved since late 1950s and is undergoing advancement in leading academic institutions worldwide. This paper delineates an original study on the world proliferation of bioengineering/biomedical engineering education and bears the name 'Project Alexander the Great'. The initial step of the project was to survey all 10448 universities, recognized by the International Association of Universities, spread among the 193 member states of the United Nations within the six continents. The project aims at identifying, disseminating, and networking, through the world-wide-web, those institutions of higher learning that provide bioengineering/biomedical engineering education. The significance of this project is multifold: i) the inception of a web-based 'world-map' in bioengineering/biomedical engineering education for the potential international student desiring to pursue a career in this field; ii) the global networking of bioengineering/biomedical engineering academic/research programs; iii) the promotion of first-class bioengineering/biomedical engineering education and the catalysis of global proliferation of this field; iv) the erection of bridges among educational institutions, industry, and professional societies or organizations involved in Bioengineering/Biomedical Engineering; and v) the catalysis in the establishment of framework agreements for cooperation among the identified institutions offering curricula in this field. This paper presents the results obtained from Africa and North America. The whole project is due to be completed by 2009.

  15. A Machine Learning Approach to Estimate Riverbank Geotechnical Parameters from Sediment Particle Size Data

    Science.gov (United States)

    Iwashita, Fabio; Brooks, Andrew; Spencer, John; Borombovits, Daniel; Curwen, Graeme; Olley, Jon

    2015-04-01

    Assessing bank stability using geotechnical models traditionally involves the laborious collection of data on the bank and floodplain stratigraphy, as well as in-situ geotechnical data for each sedimentary unit within a river bank. The application of geotechnical bank stability models are limited to those sites where extensive field data has been collected, where their ability to provide predictions of bank erosion at the reach scale are limited without a very extensive and expensive field data collection program. Some challenges in the construction and application of riverbank erosion and hydraulic numerical models are their one-dimensionality, steady-state requirements, lack of calibration data, and nonuniqueness. Also, numerical models commonly can be too rigid with respect to detecting unexpected features like the onset of trends, non-linear relations, or patterns restricted to sub-samples of a data set. These shortcomings create the need for an alternate modelling approach capable of using available data. The application of the Self-Organizing Maps (SOM) approach is well-suited to the analysis of noisy, sparse, nonlinear, multidimensional, and scale-dependent data. It is a type of unsupervised artificial neural network with hybrid competitive-cooperative learning. In this work we present a method that uses a database of geotechnical data collected at over 100 sites throughout Queensland State, Australia, to develop a modelling approach that enables geotechnical parameters (soil effective cohesion, friction angle, soil erodibility and critical stress) to be derived from sediment particle size data (PSD). The model framework and predicted values were evaluated using two methods, splitting the dataset into training and validation set, and through a Bootstrap approach. The basis of Bootstrap cross-validation is a leave-one-out strategy. This requires leaving one data value out of the training set while creating a new SOM to estimate that missing value based on the

  16. Field experiment on excess air formation in the riverbank of Danube

    International Nuclear Information System (INIS)

    Palcsu, L.; Major, Z.; Aeschbach-Hertig, W.

    2007-01-01

    Complete text of publication follows. Excess air is a contribution to the gases dissolved in groundwater in addition to the solubility equilibrium component, formed by partial or total dissolution of air trapped during water level rises in the unsaturated zone. The amount of excess air can be quite large, mainly if the water level increase is significant. The first aim of this field experiment carried out in September 2007 during a flood (Fig. 1/b) occurred along the Danube River has been to demonstrate that the excess air formation can be detected and studied under natural conditions when the groundwater level is rising. The study site is located in the riverbank of Danube River, the dug-well is at around 30 m from the riverbed (Fig. 1/a). The whole area is composed of fluvial sand. Separated water samples can be taken from the sampling tubes. Each sampling tube represents a certain depth. The horizontal groundwater flow velocity is supposed to be around 10 m/day (or at least this magnitude), that of course depends on the level difference between Danube and the groundwater. Helium and neon concentrations have been measured from the water samples. What we can see from these noble gas data is a probable correlation between groundwater level and excess noble gas concentrations. He and Ne are correlating providing evidence that the excess components are of same origin. However, the helium excess is usually larger than that of neon that means there has to be non atmospheric components of helium, such as terrigenic and tritiogenic. The R/Ra ratios are generally higher than one clearly showing tritiogenic 3 He in the water. Having a look at Fig. 1/b-c, it can be observed that there is a relatively good relation between groundwater rising and the excesses of the noble gases. From this sampling campaign, we can conclude that this kind of field experiment does make sense. Further noble gases have to be measured, and more information has to be gained regarding to the local

  17. Engineering excellence in breakthrough biomedical technologies: bioengineering at the University of California, Riverside.

    Science.gov (United States)

    Schultz, Jane S; Rodgers, V G J

    2012-07-01

    The Department of Bioengineering at the University of California, Riverside (UCR), was established in 2006 and is the youngest department in the Bourns College of Engineering. It is an interdisciplinary research engine that builds strength from highly recognized experts in biochemistry, biophysics, biology, and engineering, focusing on common critical themes. The range of faculty research interests is notable for its diversity, from the basic cell biology through cell function to the physiology of the whole organism, each directed at breakthroughs in biomedical devices for measurement and therapy. The department forges future leaders in bioengineering, mirroring the field in being energetic, interdisciplinary, and fast moving at the frontiers of biomedical discoveries. Our educational programs combine a solid foundation in bio logical sciences and engineering, diverse communication skills, and training in the most advanced quantitative bioengineering research. Bioengineering at UCR also includes the Bioengineering Interdepartmental Graduate (BIG) program. With its slogan Start-Grow-Be-BIG, it is already recognized for its many accomplishments, including being third in the nation in 2011 for bioengineering students receiving National Science Foundation graduate research fellowships as well as being one of the most ethnically inclusive programs in the nation.

  18. Preliminary assessment of bioengineered fringing shoreline reefs in Grand Isle and Breton Sound, Louisiana

    Science.gov (United States)

    La Peyre, Megan K.; Schwarting, Lindsay; Miller, Shea

    2013-01-01

    Restoration of three-dimensional shell habitats in coastal Louisiana presents a valuable and potentially self-sustaining approach to providing shoreline protection and critical nekton habitat and may contribute to water quality maintenance. The use of what has been called “living shorelines” is particularly promising because in addition to the hypothesized shoreline protection services, it is predicted that, if built and located in viable sites, these living shorelines may ultimately contribute to water quality maintenance through filtration of bivalves and may enhance nekton habitat. This approach, however, has not been tested extensively in different shallow water estuarine settings; understanding under what conditions a living shoreline must have to support a sustainable oyster population, and where these reefs may provide valuable shoreline protection, is key to ensuring that this approach provides an effective tool for coastal restoration. This project gathered preliminary data on the sustainability and shoreline stabilization of three large bioengineered fringing reefs located in Grand Isle, Lake Eloi, and Lake Fortuna, Louisiana. We collected preconstruction and postconstruction physiochemical and biological data by using a before-after-control-impact approach to evaluate the effectiveness of these living shoreline structures on reducing marsh erosion, enabling reef sustainability, and providing other ecosystem benefits. Although this project was originally designed to compare reef performance and impacts across three different locations over 2 years, delays in construction because of the Deepwater Horizon oil spill resulted in reefs being built from 12 to 18 months later than anticipated. As a result, monitoring postconstruction was severely limited. One reef, Grand Isle, was completed in March 2011 and monitored up to 18 months postcreation, whereas Lake Eloi and Lake Fortuna reefs were not completed until January 2012, and only 8 months of

  19. PREFACE: XVII Congress of Bioengineering and VI Clinical Engineering Conference

    Science.gov (United States)

    Rocha, Darío

    2011-09-01

    SABI 2009 was the XVII Biennial Congress of the Argentinean Bioengineering Society (SABI - www.sabi.org.ar), celebrated along with the VI Clinical Engineering Conference. It took place in Rosario, the second city of Argentina, located on the west bank of the Paraná, one of the world's most important rivers. This city, with its 150 year history and one million inhabitants, is characterized by a strong enterprising spirit. It is the agroindustrial leader of Argentina, with cereal ports recognized to be among the most active in the world, and its cereal stock exchange competes with Chicago's in international cereal pricing. Demographically Rosario presents a European profile, and there are seven national and private higher level universities in the area. SABI 2009 was the first time the Congress was celebrated in Rosario. Usually the Congress is organized by the Bioengineering Society in cooperation with a university with an undergraduate program, which Rosario lacks. To meet the needs of this exceptional case, a young local institution was asked to coordinate the Congress, the Rosario Technological Center (www.polotecnologico.net). This organization gathers together around 100 companies that produce technology, with a large number focused on IT, but those focused on biotechnology also stand out. The Center is also integrated with relevant public and government bodies. Traditionally, bioengineering has been related to human health applications, with less emphasis on applications significant to agrotechnology, an area in which Rosario is growing as an economic force. In order to address this oversight, the Congress formulated its main goals for integrating and synergizing bioengineering and biotechnology, particularly bioengineering and agrotechnology. This initiative has produced promising results. The importance of the Congress was reflected in the high number of participants - including researchers, professionals and students - from abroad, with participants from

  20. Design of the monitoring system at the Sant'Alessio induced riverbank filtration plant (Lucca, Italy)

    Science.gov (United States)

    Rossetto, Rudy; Barbagli, Alessio; Borsi, Iacopo; Mazzanti, Giorgio; Picciaia, Daniele; Vienken, Thomas; Bonari, Enrico

    2015-04-01

    In Managed Aquifer Recharge (MAR) schemes the monitoring system, for both water quality and quantity issues, plays a key role in assuring that a groundwater recharge plant is really managed. Considering induced Riverbank Filtration (RBF) schemes, while the effect of the augmented filtration consists in an improvement of the quality and quantity of the water infiltrating the aquifer, there is in turn the risk for groundwater contamination, as surface water bodies are highly susceptible to contamination. Within the framework of the MARSOL (2014) EU FPVII-ENV-2013 project, an experimental monitoring system has been designed and will be set in place at the Sant'Alessio RBF well field (Lucca, Italy) to demonstrate the sustainability and the benefits of managing induced RBF versus the unmanaged option. The RBF scheme in Sant'Alessio (Borsi et al. 2014) allows abstraction of an overall amount of about 0,5 m3/s groundwater providing drinking water for about 300000 people of the coastal Tuscany. Water is derived by ten vertical wells set along the Serchio River embankments inducing river water filtration into a high yield (10-2m2/s transmissivity) sand and gravel aquifer. Prior to the monitoring system design, a detailed site characterization has been completed taking advantage of previous and new investigations, the latter performed by means of MOSAIC on-site investigation platform (UFZ). A monitoring network has been set in place in the well field area using existing wells. There groundwater head and the main physico-chemical parameters (temperature, pH, dissolved oxygen, electrical conductivity and redox potential) are routinely monitored. Major geochemical compounds along with a large set of emerging pollutants are analysed (in cooperation with IWW Zentrum Wasser, Germany) both in surface-water and ground-water. The experimental monitoring system (including sensors in surface- and ground-water) has been designed focusing on managing abstraction efficiency and safety at

  1. Life cycle performances of log wood applied for soil bioengineering constructions

    Science.gov (United States)

    Kalny, Gerda; Strauss-Sieberth, Alexandra; Strauss, Alfred; Rauch, Hans Peter

    2016-04-01

    Nowadays there is a high demand on engineering solutions considering not only technical aspects but also ecological and aesthetic values. Soil bioengineering is a construction technique that uses biological components for hydraulic and civil engineering solutions. Soil bioengineering solutions are based on the application of living plants and other auxiliary materials including among others log wood. This kind of construction material supports the soil bioengineering system as long as the plants as living construction material overtake the stability function. Therefore it is important to know about the durability and the degradation process of the wooden logs to retain the integral performance of a soil bio engineering system. These aspects will be considered within the framework of the interdisciplinary research project „ELWIRA Plants, wood, steel and concrete - life cycle performances as construction materials". Therefore field investigations on soil bioengineering construction material, specifically European Larch wood logs, of different soil bioengineering structures at the river Wien have been conducted. The drilling resistance as a parameter for particular material characteristics of selected logs was measured and analysed. The drilling resistance was measured with a Rinntech Resistograph instrument at different positions of the wooden logs, all surrounded with three different backfills: Fully surrounded with air, with earth contact on one side and near the water surface in wet-dry conditions. The age of the used logs ranges from one year old up to 20 year old. Results show progress of the drilling resistance throughout the whole cross section as an indicator to assess soil bioengineering construction material. Logs surrounded by air showed a higher drilling resistance than logs with earth contact and the ones exposed to wet-dry conditions. Hence the functional capability of wooden logs were analysed and discussed in terms of different levels of degradation

  2. Islet grafting and imaging in a bioengineered intramuscular space.

    Science.gov (United States)

    Witkowski, Piotr; Sondermeijer, Hugo; Hardy, Mark A; Woodland, David C; Lee, Keagan; Bhagat, Govind; Witkowski, Kajetan; See, Fiona; Rana, Abbas; Maffei, Antonella; Itescu, Silviu; Harris, Paul E

    2009-11-15

    Because the hepatic portal system may not be the optimal site for islet transplantation, several extrahepatic sites have been studied. Here, we examine an intramuscular transplantation site, bioengineered to better support islet neovascularization, engraftment, and survival, and we demonstrate that at this novel site, grafted beta cell mass may be quantitated in a real-time noninvasive manner by positron emission tomography (PET) imaging. Streptozotocin-induced rats were pretreated intramuscularly with a biocompatible angiogenic scaffold received syngeneic islet transplants 2 weeks later. The recipients were monitored serially by blood glucose and glucose tolerance measurements and by PET imaging of the transplant site with [11C] dihydrotetrabenazine. Parallel histopathologic evaluation of the grafts was performed using insulin staining and evaluation of microvasularity. Reversal of hyperglycemia by islet transplantation was most successful in recipients pretreated with bioscaffolds containing angiogenic factors when compared with those who received no bioscaffolds or bioscaffolds not treated with angiogenic factors. PET imaging with [11C] dihydrotetrabenazine, insulin staining, and microvascular density patterns were consistent with islet survival, increased levels of angiogenesis, and with reversal of hyperglycemia. Induction of increased neovascularization at an intramuscular site significantly improves islet transplant engraftment and survival compared with controls. The use of a nonhepatic transplant site may avoid intrahepatic complications and permit the use of PET imaging to measure and follow transplanted beta cell mass in real time. These findings have important implications for effective islet implantation outside of the liver and offer promising possibilities for improving islet survival, monitoring, and even prevention of islet loss.

  3. Development of bioengineering system for stem cell proliferation

    Science.gov (United States)

    Park, H. S.; Shah, R.; Shah, C.

    2016-08-01

    From last decades, intensive research in the field of stem cells proliferation had been promoted due to the unique property of stem cells to self-renew themselves into multiples and has potential to replicate into an organ or tissues and so it's highly demanding though challenging. Bioreactor, a mechanical device, works as a womb for stem cell proliferation by providing nutritious environment for the proper growth of stem cells. Various factors affecting stem cells growth are the bioreactor mechanism, feeding of continuous nutrients, healthy environment, etc., but it always remains a challenge for controlling biological parameters. The present paper unveils the design of mechanical device commonly known as bioreactor in tissues engineering and biotech field, use for proliferation of stem cells and imparts the proper growing condition for stem cells. This high functional bioreactor provides automation mixing of cell culture and stem cells. This design operates in conjunction with mechanism of reciprocating motion. Compare to commercial bioreactors, this proposed design is more convenient, easy to operate and less maintenance is required as bioreactor culture bag is made of polyethylene which is single use purpose. Development of this bioengineering system will be beneficial for better growth and expansion of stem cell

  4. Bioengineered protein-based nanocage for drug delivery.

    Science.gov (United States)

    Lee, Eun Jung; Lee, Na Kyeong; Kim, In-San

    2016-11-15

    Nature, in its wonders, presents and assembles the most intricate and delicate protein structures and this remarkable phenomenon occurs in all kingdom and phyla of life. Of these proteins, cage-like multimeric proteins provide spatial control to biological processes and also compartmentalizes compounds that may be toxic or unstable and avoids their contact with the environment. Protein-based nanocages are of particular interest because of their potential applicability as drug delivery carriers and their perfect and complex symmetry and ideal physical properties, which have stimulated researchers to engineer, modify or mimic these qualities. This article reviews various existing types of protein-based nanocages that are used for therapeutic purposes, and outlines their drug-loading mechanisms and bioengineering strategies via genetic and chemical functionalization. Through a critical evaluation of recent advances in protein nanocage-based drug delivery in vitro and in vivo, an outlook for de novo and in silico nanocage design, and also protein-based nanocage preclinical and future clinical applications will be presented. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Bioengineering in renal transplantation: technological advances and novel options.

    Science.gov (United States)

    Yeo, Wee-Song; Zhang, Yao-Chun

    2017-06-06

    End-stage kidney disease (ESKD) is one of the most prevalent diseases in the world with significant morbidity and mortality. Current modes of renal replacement therapy include dialysis and renal transplantation. Although dialysis is an acceptable mode of renal replacement therapy, it does have its shortcomings, which include poorer life expectancy compared with renal transplantation, risk of infections and vascular thrombosis, lack of vascular access and absence of biosynthetic functions of the kidney. Renal transplantation, in contrast, is the preferred option of renal replacement therapy, with improved morbidity and mortality rates and quality of life, compared with dialysis. Renal transplantation, however, may not be available to all patients with ESKD. Some of the key factors limiting the availability and efficiency of renal transplantation include shortage of donor organs and the constant risk of rejection with complications associated with over-immunosuppression respectively. This review focuses chiefly on the potential roles of bioengineering in overcoming limitations in renal transplantation via the development of cell-based bioartificial dialysis devices as bridging options before renal transplantation, and the development of new sources of organs utilizing cell and organ engineering.

  6. Leonardo da Vinci: engineer, bioengineer, anatomist, and artist.

    Science.gov (United States)

    West, John B

    2017-03-01

    Leonardo da Vinci (1452-1519) enjoys a reputation as one of the most talented people of all time in the history of science and the arts. However, little attention has been given to his contributions to physiology. One of his main interests was engineering, and he was fascinated by structural problems and the flow patterns of liquids. He also produced a large number of ingenious designs for warfare and a variety of highly original flying machines. But of particular interest to us are his contributions to bioengineering and how he used his knowledge of basic physical principles to throw light on physiological function. For example, he produced new insights into the mechanics of breathing including the action of the ribs and diaphragm. He was the first person to understand the different roles of the internal and external intercostal muscles. He had novel ideas about the airways including the mode of airflow in them. He also worked on the cardiovascular system and had a special interest in the pulmonary circulation. But, interestingly, he was not able to completely divorce his views from those of Galen, in that although he could not see pores in the interventricular septum of the heart, one of his drawings included them. Leonardo was a talented anatomist who made many striking drawings of the human body. Finally, his reputation for many people is based on his paintings including the Mona Lisa that apparently attracts more viewers than any other painting in the world. Copyright © 2017 the American Physiological Society.

  7. Modelling tools for managing Induced RiverBank Filtration MAR schemes

    Science.gov (United States)

    De Filippis, Giovanna; Barbagli, Alessio; Marchina, Chiara; Borsi, Iacopo; Mazzanti, Giorgio; Nardi, Marco; Vienken, Thomas; Bonari, Enrico; Rossetto, Rudy

    2017-04-01

    Induced RiverBank Filtration (IRBF) is a widely used technique in Managed Aquifer Recharge (MAR) schemes, when aquifers are hydraulically connected with surface water bodies, with proven positive effects on quality and quantity of groundwater. IRBF allows abstraction of a large volume of water, avoiding large decrease in groundwater heads. Moreover, thanks to the filtration process through the soil, the concentration of chemical species in surface water can be reduced, thus becoming an excellent resource for the production of drinking water. Within the FP7 MARSOL project (demonstrating Managed Aquifer Recharge as a SOLution to water scarcity and drought; http://www.marsol.eu/), the Sant'Alessio IRBF (Lucca, Italy) was used to demonstrate the feasibility and technical and economic benefits of managing IRBF schemes (Rossetto et al., 2015a). The Sant'Alessio IRBF along the Serchio river allows to abstract an overall amount of about 0.5 m3/s providing drinking water for 300000 people of the coastal Tuscany (mainly to the town of Lucca, Pisa and Livorno). The supplied water is made available by enhancing river bank infiltration into a high yield (10-2 m2/s transmissivity) sandy-gravelly aquifer by rising the river head and using ten vertical wells along the river embankment. A Decision Support System, consisting in connected measurements from an advanced monitoring network and modelling tools was set up to manage the IRBF. The modelling system is based on spatially distributed and physically based coupled ground-/surface-water flow and solute transport models integrated in the FREEWAT platform (developed within the H2020 FREEWAT project - FREE and Open Source Software Tools for WATer Resource Management; Rossetto et al., 2015b), an open source and public domain GIS-integrated modelling environment for the simulation of the hydrological cycle. The platform aims at improving water resource management by simplifying the application of EU water-related Directives and at

  8. The riverbank filtration plant in S. Alessio (Lucca: monitoring and modeling activity within EU the FP7 MARSOL project

    Directory of Open Access Journals (Sweden)

    Iacopo Borsi

    2014-09-01

    Full Text Available The riverbank filtration (RBF scheme along the Serchio River, Lucca (Tuscany – Italy allows abstraction of an overall amount of about 1 m3/s groundwater providing drinking water for about 300000 people of the coastal Tuscany (town of Lucca, Pisa and Livorno. Water is derived by means of an RBF scheme by a set of ten vertical wells inducing riverbank filtration into a high yield (10-2 m2/s transmissivity sand and gravel aquifer including a downstream weir to raise river head and increasing water storage in the aquifer along the river reach. Within the framework of the MARSOL FPVII-ENV-2013 project, the Sant’Alessio well field will be used to demonstrate the sustainability, by a technical, social and market point of view, and the benefits of RBF managing versus the unmanaged option. The Serchio experimental site will involve merging existing and proved technologies, including continuous monitoring of several parameters and analytes and the development of dedicated software tools, to produce a Decision Support System (DSS based on remote data acquisition and transmission and GIS physically-based fully distributed numerical modeling to continuously monitor and manage the well field, reducing also, prone to error, human operated activities. A set of sensors will be installed to monitor by a quantitative and qualitative point of view hydrologic variables in the river water, in the aquifer, the unsaturated zone and the wells. Data will be continuously acquired and remotely transmitted to a server where they will first be checked for consistency and then sent to a database for processing in a dedicated modelling environment included in the DSS and equipped with an alert system to inform water managers about the scheme performance and reaching limits of infiltration rates or water quality indices. The DSS along with the installed sensors, data transmission and storage tools will constitute a prototype whose potential market exploitation will be

  9. Functionalized bioengineered spider silk spheres improve nuclease resistance and activity of oligonucleotide therapeutics providing a strategy for cancer treatment.

    Science.gov (United States)

    Kozlowska, Anna Karolina; Florczak, Anna; Smialek, Maciej; Dondajewska, Ewelina; Mackiewicz, Andrzej; Kortylewski, Marcin; Dams-Kozlowska, Hanna

    2017-09-01

    Cell-selective delivery and sensitivity to serum nucleases remain major hurdles to the clinical application of RNA-based oligonucleotide therapeutics, such as siRNA. Spider silk shows great potential as a biomaterial due to its biocompatibility and biodegradability. Self-assembling properties of silk proteins allow for processing into several different morphologies such as fibers, scaffolds, films, hydrogels, capsules and spheres. Moreover, bioengineering of spider silk protein sequences can functionalize silk by adding peptide moieties with specific features including binding or cell recognition domains. We demonstrated that modification of silk protein by adding the nucleic acid binding domain enabled the development of a novel oligonucleotide delivery system that can be utilized to improve pharmacokinetics of RNA-based therapeutics, such as CpG-siRNA. The MS2 bioengineered silk was functionalized with poly-lysine domain (KN) to generate hybrid silk MS2KN. CpG-siRNA efficiently bound to MS2KN in contrary to control MS2. Both MS2KN complexes and spheres protected CpG-siRNA from degradation by serum nucleases. CpG-siRNA molecules encapsulated into MS2KN spheres were efficiently internalized and processed by TLR9-positive macrophages. Importantly, CpG-STAT3siRNA loaded in silk spheres showed delayed and extended target gene silencing compared to naked oligonucleotides. The prolonged Stat3 silencing resulted in the more pronounced downregulation of interleukin 6 (IL-6), a proinflammatory cytokine and upstream activator of STAT3, which limits the efficacy of TLR9 immunostimulation. Our results demonstrate the feasibility of using spider silk spheres as a carrier of therapeutic nucleic acids. Moreover, the modified kinetic and activity of the CpG-STAT3siRNA embedded into silk spheres is likely to improve immunotherapeutic effects in vivo. We demonstrated that modification of silk protein by adding the nucleic acid binding domain enabled the development of a novel

  10. Bioengineering of Dental Tissues: Bibliometric Analysis 2000-2011.

    Directory of Open Access Journals (Sweden)

    Sebastián Zamorano

    2014-03-01

    Full Text Available Introduction: There has been a noticeable increase in experimental use and therapies based on stem cells over recent years. Nevertheless, there is a lack of information about this progress in the dental field, which makes it difficult to trace development and design policies. The purpose of this study, as a first approach to the subject, is to determine a bibliometric profile for the investigation related to bioengineering of dental tissue at a worldwide scale, based on the MEDLINE database, for the period 2000-2011. Methodology: A bibliometric study was carried out. Every article indexed in the MEDLINE database and associated with the terms “stem cells” and “tooth regeneration” for the period 2000-2011 was included. The analyzed variables were publishing date, country of origin, language and publication type (original or review, journal, author, associated university and tissue source (human or animal. Results: For the entire period included in the study, 257 articles were found. Of these, 149 corresponded to original Works published in English; 5 in other languages; 92 comprised literature reviews in English, 9 in other languages and 2 publications were included in the “others” category. The countries with the highest research productivity were the United States (24.51%, Japan (20.62% and China (17.90%, while Brazil (3.9% was the only Latin-American country found in the list. Animal tissues were used in 59.09% of them. The most productive authors were Ueda M (17 and Jin Y (11, whereas Fourth Military University (13, University of Tokyo (12 and Capital Medical University (10 had the largest number of publications. Conclusion: The United States, Japan and China concentrate about two thirds of the production. Latin-America was represented only by Brazil.

  11. Islet grafting and imaging in a bioengineered intramuscular space†

    Science.gov (United States)

    Witkowski, Piotr; Sondermeijer, Hugo; Hardy, Mark A.; Woodland, David C.; Lee, Keagan; Bhagat, Govind; Witkowski, Kajetan; See, Fiona; Rana, Abbas; Maffei, Antonella; Itescu, Silviu; Harris, Paul E.

    2011-01-01

    Background Since the hepatic portal system may not be the optimal site for islet transplantation, several extrahepatic sites have been studied. Here we examine an intramuscular transplantation site, bioengineered to better support islet neovascularization, engraftment, and survival, and demonstrate that at this novel site, grafted beta cell mass may be quantitated in a real time non-invasive manner by PET imaging. Methods Streptozotocin induced rats were pretreated intramuscularly with a biocompatible angiogenic scaffold received syngeneic islet transplants 2 weeks later. The recipients were monitored serially by blood glucose and glucose tolerance measurements and by PET imaging of the transplant site with [11C] dihydrotetrabenazine. Parallel histopathologic evaluation of the grafts was done using insulin staining and evaluation of microvasularity. Results Reversal of hyperglycemia by islet transplantation was most successful in recipients pretreated with bioscaffolds containing angiogenic factors as compared to those who received no bioscaffolds or bioscaffolds not treated with angiogenic factors. PET imaging with [11C] dihydrotetrabenazine, insulin staining and microvascular density patterns were consistent with islet survival, increased levels of angiogenesis, and with reversal of hyperglycemia. Conclusions Induction of increased neovascularization at an intramuscular site significantly improves islet transplant engraftment and survival compared to controls. The use of a non hepatic transplant site may avoid intrahepatic complications and permit the use of PET imaging to measure and follow transplanted beta-cell mass in real time. These findings have important implications for effective islet implantation outside of the liver, and offer promising possibilities for improving islet survival, monitoring, and even prevention of islet loss. PMID:19898201

  12. Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies

    Directory of Open Access Journals (Sweden)

    Gregory H. Underhill

    2018-01-01

    Full Text Available In vitro models of the human liver are important for the following: (1 mitigating the risk of drug-induced liver injury to human beings, (2 modeling human liver diseases, (3 elucidating the role of single and combinatorial microenvironmental cues on liver cell function, and (4 enabling cell-based therapies in the clinic. Methods to isolate and culture primary human hepatocytes (PHHs, the gold standard for building human liver models, were developed several decades ago; however, PHHs show a precipitous decline in phenotypic functions in 2-dimensional extracellular matrix–coated conventional culture formats, which does not allow chronic treatment with drugs and other stimuli. The development of several engineering tools, such as cellular microarrays, protein micropatterning, microfluidics, biomaterial scaffolds, and bioprinting, now allow precise control over the cellular microenvironment for enhancing the function of both PHHs and induced pluripotent stem cell–derived human hepatocyte-like cells; long-term (4+ weeks stabilization of hepatocellular function typically requires co-cultivation with liver-derived or non–liver-derived nonparenchymal cell types. In addition, the recent development of liver organoid culture systems can provide a strategy for the enhanced expansion of therapeutically relevant cell types. Here, we discuss advances in engineering approaches for constructing in vitro human liver models that have utility in drug screening and for determining microenvironmental determinants of liver cell differentiation/function. Design features and validation data of representative models are presented to highlight major trends followed by the discussion of pending issues that need to be addressed. Overall, bioengineered liver models have significantly advanced our understanding of liver function and injury, which will prove useful for drug development and ultimately cell-based therapies.

  13. Skin Hydration Assessment through Modern Non-Invasive Bioengineering Technologies.

    Science.gov (United States)

    Constantin, Maria-Magdalena; Poenaru, Elena; Poenaru, Calin; Constantin, Traian

    2014-03-01

    Non-invasive bioengineering technologies continuously discovered and developed in recent decades provide a significant input to research development and remarkably contribute to the improvement of medical education and care to our patients. Assessing skin hydration by using the capacitance method for a group of patients with allergic contact dermatitis versus healthy subjects, before and after applying a moisturiser (assessing the immediate and long-term effectiveness of hydration). For both groups, but especially for the patients with dry skin, there was a clear improvement of hydration, statistically significant after applying the moisturiser. In the case of the patients with allergic contact dermatitis, hydration was at a maximum immediately after the first application, and then maintained an increased level after 7 and 28 days, respectively. In the healthy subjects, the increase in hydration was lower, but progressive. The moisturiser determined an increase in hydration for all age groups, but those who showed the most obvious effect were the young adults (18-29 years old) with an increase of 19.9%.The maintenance effect of hydration lasted for 28 days, while the improvement was important for allergic skin (17.1%) and significant for healthy skin (10.9%). The assessment of epidermal hydration performed by using the corneometer showed very good hydration of the stratum corneum for both groups studied, with immediate and long-term effect. This study also showed that the degree of skin hydration was inversely proportional with age. The corneometer is easy to use, efficient and widely utilised in international studies for measurements in healthy or pathological conditions, for quantitative assessment of the effectiveness of various preparations intended for application to the skin surface, under well-controlled and standardised conditions.

  14. Protective

    Directory of Open Access Journals (Sweden)

    Wessam M. Abdel-Wahab

    2013-10-01

    Full Text Available Many active ingredients extracted from herbal and medicinal plants are extensively studied for their beneficial effects. Antioxidant activity and free radical scavenging properties of thymoquinone (TQ have been reported. The present study evaluated the possible protective effects of TQ against the toxicity and oxidative stress of sodium fluoride (NaF in the liver of rats. Rats were divided into four groups, the first group served as the control group and was administered distilled water whereas the NaF group received NaF orally at a dose of 10 mg/kg for 4 weeks, TQ group was administered TQ orally at a dose of 10 mg/kg for 5 weeks, and the NaF-TQ group was first given TQ for 1 week and was secondly administered 10 mg/kg/day NaF in association with 10 mg/kg TQ for 4 weeks. Rats intoxicated with NaF showed a significant increase in lipid peroxidation whereas the level of reduced glutathione (GSH and the activity of superoxide dismutase (SOD, catalase (CAT, glutathione S-transferase (GST and glutathione peroxidase (GPx were reduced in hepatic tissues. The proper functioning of the liver was also disrupted as indicated by alterations in the measured liver function indices and biochemical parameters. TQ supplementation counteracted the NaF-induced hepatotoxicity probably due to its strong antioxidant activity. In conclusion, the results obtained clearly indicated the role of oxidative stress in the induction of NaF toxicity and suggested hepatoprotective effects of TQ against the toxicity of fluoride compounds.

  15. Development of a tissue engineered heart valve for pediatrics: a case study in bioengineering ethics.

    Science.gov (United States)

    Merryman, W David

    2008-03-01

    The following hypothetical case study was developed for bioengineering students and is concerned with choosing between two devices used for development of a pediatric tissue engineered heart valve (TEHV). This case is intended to elicit assessment of the devices, possible future outcomes, and ramifications of the decision making. It is framed in light of two predominant ethical theories: utilitarianism and rights of persons. After the case was presented to bioengineering graduate students, they voted on which device should be released. The results revealed that these bioengineering students preferred the more reliable (and substantially more expensive) design, though this choice precludes the majority of the world from having access to this technology. This case is intended to examine and explore where the balance lies between design, cost, and adequate distribution of biomedical devices.

  16. Perianal implantation of bioengineered human internal anal sphincter constructs intrinsically innervated with human neural progenitor cells.

    Science.gov (United States)

    Raghavan, Shreya; Miyasaka, Eiichi A; Gilmont, Robert R; Somara, Sita; Teitelbaum, Daniel H; Bitar, Khalil N

    2014-04-01

    The internal anal sphincter (IAS) is a major contributing factor to pressure within the anal canal and is required for maintenance of rectoanal continence. IAS damage or weakening results in fecal incontinence. We have demonstrated that bioengineered, intrinsically innervated, human IAS tissue replacements possess key aspects of IAS physiology, such as the generation of spontaneous basal tone and contraction/relaxation in response to neurotransmitters. The objective of this study is to demonstrate the feasibility of implantation of bioengineered IAS constructs in the perianal region of athymic rats. Human IAS tissue constructs were bioengineered from isolated human IAS circular smooth muscle cells and human enteric neuronal progenitor cells. After maturation of the bioengineered constructs in culture, they were implanted operatively into the perianal region of athymic rats. Platelet-derived growth factor was delivered to the implanted constructs through a microosmotic pump. Implanted constructs were retrieved from the animals 4 weeks postimplantation. Animals tolerated the implantation well, and there were no early postoperative complications. Normal stooling was observed during the implantation period. At harvest, implanted constructs were adherent to the perirectal rat tissue and appeared healthy and pink. Immunohistochemical analysis revealed neovascularization. Implanted smooth muscle cells maintained contractile phenotype. Bioengineered constructs responded in vitro in a tissue chamber to neuronally evoked relaxation in response to electrical field stimulation and vasoactive intestinal peptide, indicating the preservation of neuronal networks. Our results indicate that bioengineered innervated IAS constructs can be used to augment IAS function in an animal model. This is a regenerative medicine based therapy for fecal incontinence that would directly address the dysfunction of the IAS muscle. Copyright © 2014 Mosby, Inc. All rights reserved.

  17. Managing induced riverbank filtration (IRF) at the Serchio River well field, Tuscany, Italy (Italy)

    Science.gov (United States)

    Rossetto, Rudy; Ansiati, Alberto; Barbagli, Alessio; Borsi, Iacopo; Costabile, Gennarino; Dietrich, Peter; Mazzanti, Giorgio; Picciaia, Daniele; Bonari, Enrico

    2014-05-01

    Along the Serchio River (Tuscany -Italy) a series of well fields is set for an overall amount of about 1 m3/s pumped groundwater providing drinking water for about 300000 people of the coastal Tuscany (mainly to the town of Lucca, Pisa and Livorno). Water is pumped enhancing riverbank filtration into a high yield (10-2 m2/s transmissivity) sand and gravel aquifer by artificially rising river head and setting pumping well fields along the river reach. However, being it unmanaged aquifer recharge, concerns arise both for quality and quantity of the abstracted groundwater. It happens in dry climate extremes (i.e. 2002/2003 or 2011/2012) that Serchio River flow falls below minimum environmental flow (MEF). Long term contamination of river water had been causing contamination of groundwater, as in 2002/2006, when pesticide contaminated surface water was polluting the well fields causing several problems to water supply. Such problems were overcome by setting in place derogatory regulations and then through dissemination and stakeholder activities reducing pesticide presence in surface water (EU LIFE SERIAL WELLFIR project). Although widely adopted, IRF is also not well stated from a regulatory point of view, eventually leading to concerns by a legal point of view. Within the framework of the MARSOL FPVII-ENV-2013 project an experimental site at a well field will be set to demonstrate the feasibility (by a technical, social and market point of view) and the benefits of managing IRF versus the unmanaged option. The Serchio experimental site will involve merging existing and proved technologies to produce a Decision Support System (DSS) based on remote data acquisition and transmission and GIS physically-based fully distributed numerical modeling to continuously monitor and manage well fields, reducing also human operated activities. The DSS along with the installed sensors, data transmission and storage tools will constitute a prototype whose potential market exploitation

  18. An Effective Numerical Method and Its Utilization to Solution of Fractional Models Used in Bioengineering Applications

    Directory of Open Access Journals (Sweden)

    Petráš Ivo

    2011-01-01

    Full Text Available This paper deals with the fractional-order linear and nonlinear models used in bioengineering applications and an effective method for their numerical solution. The proposed method is based on the power series expansion of a generating function. Numerical solution is in the form of the difference equation, which can be simply applied in the Matlab/Simulink to simulate the dynamics of system. Several illustrative examples are presented, which can be widely used in bioengineering as well as in the other disciplines, where the fractional calculus is often used.

  19. Use of subatmospheric (VAC) therapy to improve bioengineered tissue grafting in diabetic foot wounds.

    Science.gov (United States)

    Espensen, Eric H; Nixon, Brent P; Lavery, Lawrence A; Armstrong, David G

    2002-01-01

    The use of bioengineered tissue and topical subatmospheric pressure therapy have both been widely accepted as adjunctive therapies for the treatment of noninfected, nonischemic diabetic foot wounds. This article describes a temporally overlapping method of care that includes a period of simultaneous application of bioengineered tissue (Apligraf, Novartis Pharmaceuticals Corp, East Hanover, New Jersey) and subatmospheric pressure therapy delivered through the VAC (Vacuum Assisted Closure) system (KCI, Inc, San Antonio, Texas). Future descriptive and analytic works may test the hypothesis that combined therapies used at different and often overlapping periods during the wound-healing cycle may be more effective than a single modality.

  20. Bio-Engineering High Performance Microbial Strains for MEOR

    Energy Technology Data Exchange (ETDEWEB)

    Xiangdong Fang; Qinghong Wang; Patrick Shuler

    2007-12-30

    The main objectives of this three-year research project are: (1) to employ the latest advances in genetics and bioengineering, especially Directed Protein Evolution technology, to improve the effectiveness of the microbial enhanced oil recovery (MEOR) process. (2) to improve the surfactant activity and the thermal stability of bio-surfactant systems for MEOR; and (3) to develop improved laboratory methods and tools that screen quickly candidate bio-systems for EOR. Biosurfactants have been receiving increasing attention as Enhanced Oil Recovery (EOR) agents because of their unique properties (i.e., mild production conditions, lower toxicity, and higher biodegradability) compared to their synthetic chemical counterparts. Rhamnolipid as a potent natural biosurfactant has a wide range of potential applications, including EOR and bioremediation. During the three-year of the project period, we have successfully cloned the genes involved in the rhamnolipid bio-synthesis. And by using the Transposon containing Rhamnosyltransferase gene rhlAB, we engineered the new mutant strains P. aeruginosa PEER02 and E. coli TnERAB so they can produce rhamnolipid biosurfactans. We were able to produce rhamnolipds in both P. aeroginosa PAO1-RhlA- strain and P. fluorescens ATCC15453 strain, with the increase of 55 to 175 fold in rhamnolipid production comparing with wild type bacteria strain. We have also completed the first round direct evolution studies using Error-prone PCR technique and have constructed the library of RhlAB-containing Transposon to express mutant gene in heterologous hosts. Several methods, such as colorimetric agar plate assay, colorimetric spectrophotometer assay, bioactive assay and oil spreading assay have been established to detect and screen rhamnolipid production. Our engineered P. aeruginosa PEER02 strain can produce rhamnolipids with different carbon sources as substrate. Interfacial tension analysis (IFT) showed that different rhamnolipids from different

  1. Bio-Engineering Services to the Developmentally Disabled Adolescent. Final Report.

    Science.gov (United States)

    Mallik, Kalisankar; Yuspeh, Sheldon

    A 1-year demonstration project involving 24 developmentally disabled students (9- to 20-years-old) with severe physical limitations was conducted to increase their educational and vocational possibilities by using cost-effective bio-engineering techniques to modify their physical environment and develop improved adaptive devices. Phase I of the…

  2. Considerations for restoring temperate forests of tomorrow: Forest restoration, assisted migration, and bioengineering

    Science.gov (United States)

    Kas Dumroese; Mary I. Williams; John A. Stanturf; Brad St. Clair

    2015-01-01

    Tomorrow’s forests face extreme pressures from contemporary climate change, invasive pests, and anthropogenic demands for other land uses. These pressures, collectively, demand land managers to reassess current and potential forest management practices. We discuss three considerations, functional restoration, assisted migration, and bioengineering, which are currently...

  3. Making perfect life : bio-engineering (in) the 21st century : interim study

    NARCIS (Netherlands)

    Est, van R.; Keulen, van I.; Geesink, I.; Schuiff, M.

    2010-01-01

    Reportnumber: PE 438.829; This document is the result of the preparatory phase of the STOA-project "Making perfect life", that defined the project focus. This resulted in the study document Making Perfect Life: Bio-engineering (in) the 21st century. A project plan for the next two phases was

  4. Simulation of a Congress at the Chair of Biology II in Bioengineering

    International Nuclear Information System (INIS)

    Naranjo, A V; Reznichenco, V; Lopez, N; Hernandez, R; Bajinay, S

    2007-01-01

    This work has been developed in the Chair of Biology II, the curricular contents of which correspond to Human Anatomy. This subject is taught in the second semester of the second year of studies in Bioengineering. Our main objective is that the students attending the course may integrate the syllabus contents of Anatomy with those of other subjects in the career. Ever since 1998 we have organized a congress named Congreso Intracatedra de BiologIa II (Intra Chair Congress on Biology II). This is the last assignment in the semester and is compulsory for regular students of the subject. It consists in simulating a scientific congress with international characteristics. The guidelines for the congress are made known to the students at the beginning of the semester. In groups of up to three members, the students must undertake a work that relates aspects of Anatomy with Bioengineering. Students are expected to investigate on diagnostic and/or therapeutic technology in order to write a paper that must be accepted in advance of the event. The presentation of the work must be made through PowerPoint. The originality of the research work done and the wide range of topics selected are surprising. Problems are tackled from the standpoints both of the various medical fields and of bioengineering despite the fact that they are just students of the second year in Bioengineering

  5. 77 FR 54584 - National Institute of Biomedical Imaging and Bioengineering; Notice of Closed Meeting

    Science.gov (United States)

    2012-09-05

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health National Institute of... Imaging and Bioengineering Special Emphasis Panel, ZEB1 OSR-D(J2) P Tissue Engineering Resource Center... applications. Place: Best Western Hotel III Tria, 220 Alewife Brook Parkway, Cambridge, MA 02138. Contact...

  6. Ecological and Bioengineering Studies for Stabilizing the Wad Medani-Sennar Roadside Slope Linking the Gezira and Sennar States

    Directory of Open Access Journals (Sweden)

    Altaeb Mohammed

    2018-01-01

    Full Text Available The erosion of the highway embankment slope's soil along the Wad Medani-Sennar road is a significant issue, as there are many traffic accidents on this road, with an average of 15 to 25 fatalities per annum. It was thus decided to investigate this issue to find a method to protect slope from erosion on this road and to provide new approaches to slope erosion knowledge gap in Sudan. An engineering survey was carried out, followed by geotechnical studies, experimental work and interviews with academic experts regarding native vegetation in the survey area. These include measuring the eroded parts of the road; studying cross- sections of the road; soil experiments to check the strength, compaction and particle size distribution; and a native vegetation survey to check for suitable plants that could be used to control the slope erosion. It was found that an appropriate bio-engineering method to stabilize the slope soil against erosion due to rainfall was to cultivate the grasses Cynodon Dactylon and Vetiver on the slopes. In conclusion, that using native vegetation for eco -protection, was an excellent solution to the problem based on the climate, native vegetation, and type of soil in Sudan and it reduces the accidents.

  7. Removal of iron and manganese using granular activated carbon and zeolite in artificial barrier of riverbank filtration

    Science.gov (United States)

    Ismail, Abustan; Harmuni, Halim; Mohd, Remy Rozainy M. A. Z.

    2017-04-01

    Iron and Manganese was examined from riverbank filtration (RBF) and river water in Sungai Kerian, Lubok Buntar, Serdang Kedah. Water from the RBF was influenced by geochemical and hydro chemical processes in the aquifer that made concentrations of iron (Fe), and manganese (Mn) high, and exceeded the standard values set by the Malaysia Ministry of Health. Therefore, in order to overcome the problem, the artificial barrier was proposed to improve the performance of the RBF. In this study, the capability and performance of granular activated carbon, zeolite and sand were investigated in this research. The effects of dosage, shaking speed, pH and contact time on removal of iron and manganese were studied to determine the best performance. For the removal of iron using granular activated carbon (GAC) and zeolite, the optimum contact time was at 2 hours with 200rpm shaking speed with 5g and 10g at pH 5 with percentage removal of iron was 87.81% and 83.20% respectively. The removal of manganese and zeolite arose sharply in 75 minutes with 90.21% removal, with 100rpm shaking speed. The GAC gave the best performance with 99.39% removal of manganese. The highest removal of manganese was achieved when the adsorbent dosage increased to 10g with shaking speed of 200rpm.

  8. Fluorescent microspheres as surrogates in evaluating the efficacy of riverbank filtration for removing Cryptosporidium parvum oocysts and other pathogens

    Science.gov (United States)

    Harvey, Ronald W.; Metge, David W.; Sheets, Rodney A.; Jasperse, Jay

    2011-01-01

    A major benefit of riverbank filtration (RBF) is that it provides a relatively effective means for pathogen removal. There is a need to conduct more injection-and-recovery transport studies at operating RBF sites in order to properly assess the combined effects of the site heterogeneities and ambient physicochemical conditions, which are difficult to replicate in the lab. For field transport studies involving pathogens, there is considerable interest in using fluorescent carboxylated microspheres (FCM) as surrogates, because they are chemically inert, negatively charged, easy to detect, available in a wide variety of sizes, and have been found to be nonhazardous in tracer applications. Although there have been a number of in-situ studies comparing the subsurface transport behaviors of FCM to those of bacteria and viruses, much less is known about their suitability for investigations of protozoa. Oocysts of the intestinal protozoan pathogen Cryptosporidium spp are of particular concern for many RBF operations because of their ubiquity and persistence in rivers and high resistance to chlorine disinfection. Although microspheres often have proven to be less-than-ideal analogs for capturing the abiotic transport behavior of viruses and bacteria, there is encouraging recent evidence regarding use of FCM as surrogates for C. parvum oocysts. This chapter discusses the potential of fluorescent microspheres as safe and easy-to-detect surrogates for evaluating the efficacy of RBF operations for removing pathogens, particularly Cryptosporidium, from source waters at different points along the flow path.

  9. Bioengineering of the plant culture of Capsicum frutescens with vanillin synthase gene for the production of vanillin

    OpenAIRE

    Chee, Marcus Jenn Yang; Lycett, Grantley W.; Khoo, Teng-Jin; Chin, Chiew Foan

    2016-01-01

    Production of vanillin by bioengineering has gained popularity due to consumer demand towards vanillin produced by biological systems. Natural vanillin from vanilla beans is very expensive to produce compared to its synthetic counterpart. Current bioengineering works mainly involve microbial biotechnology. Therefore, alternative means to the current approaches are constantly being explored. This work describes the use of vanillin synthase (VpVAN), to bioconvert ferulic acid to vanillin in a p...

  10. A Bioengineered Human Skin Equivalent (HSE) for the Evaluation of Protectants

    Science.gov (United States)

    2006-11-01

    agonist clofibrate to the growth media. Medium supplemented with 25 μM palmitic acid , 15 μM linoleic acid , 25 μM oleic acid , 7 μM arachidonic acid , 0.25...granules (indicated by arrows). Fig. 6: A cross section of the HSE with lipids, ascorbic acid and clofibrate supplementation. The combination of... Clofibrate , Ascorbic Acid and Lipids Compared With the Lipid Profile of Native Human Skin. Clofibrate 300 μM Lipid class Control No ascorbic

  11. Geomorphic and hydraulic controls on large-scale riverbank failure on a mixed bedrock-alluvial river system, the River Murray, South Australia: a bathymetric analysis.

    Science.gov (United States)

    De Carli, E.; Hubble, T.

    2014-12-01

    During the peak of the Millennium Drought (1997-2010) pool-levels in the lower River Murray in South Australia dropped 1.5 metres below sea level, resulting in large-scale mass failure of the alluvial banks. The largest of these failures occurred without signs of prior instability at Long Island Marina whereby a 270 metre length of populated and vegetated riverbank collapsed in a series of rotational failures. Analysis of long-reach bathymetric surveys of the river channel revealed a strong relationship between geomorphic and hydraulic controls on channel width and downstream alluvial failure. As the entrenched channel planform meanders within and encroaches upon its bedrock valley confines the channel width is 'pinched' and decreases by up to half, resulting in a deepening thalweg and channel bed incision. The authors posit that flow and shear velocities increase at these geomorphically controlled 'pinch-points' resulting in complex and variable hydraulic patterns such as erosional scour eddies, which act to scour the toe of the slope over-steepening and destabilising the alluvial margins. Analysis of bathymetric datasets between 2009 and 2014 revealed signs of active incision and erosional scour of the channel bed. This is counter to conceptual models which deem the backwater zone of a river to be one of decelerating flow and thus sediment deposition. Complex and variable flow patterns have been observed in other mixed alluvial-bedrock river systems, and signs of active incision observed in the backwater zone of the Mississippi River, United States. The incision and widening of the lower Murray River suggests the channel is in an erosional phase of channel readjustment which has implications for riverbank collapse on the alluvial margins. The prevention of seawater ingress due to barrage construction at the Murray mouth and Southern Ocean confluence, allowed pool-levels to drop significantly during the Millennium Drought reducing lateral confining support to the

  12. Effect of low-frequency electromagnetic fields on the Escherichia coli growth for application in riverbank filtration

    Science.gov (United States)

    Selamat, Rossitah; Abustan, Ismail; Arshad, Mohd Rizal; Kamal, Nurul Hana Mokhtar

    2017-10-01

    A 50 Hz low-frequency electromagnetic field (LF-EMF) could give removal effect on the growth of Escherichia coli (E. coli) in the riverbank filtration (RBF) was studied. The LF-EMF strengths were varied from 2 to 10 mT using coil column induce magnetic fields. For this study, two different columns with a diameter of 50 mm and 80 mm were applied; 1.5mm copper wires with the number of turns between 300 to 600 turns. Water samples were flowed into the column at flowrate of 50mL/min with temperature in range of 28-30°C. This study, it was found that the LF-EMF has significant removal effects on E. coli growth, where the removal of 100% of E. coli at 6 mT and 8 mT after 6 hrs exposed. Moreover, for shorter exposure period of 3 hrs exposed, 82% of E. coli removal were detected in 80 mm column for 8 mT and 10 mT magnetic field. These findings indicate that the application of LF-EMF is able to decrease the E. coli, can be and efficient in E. coli growth from river water intake. Thus, the results from this study proved that the utilization of LF-EMF is able to decrease the concentrations of E. coli, and contributes in slowing down their growth. Also, it can be indicated that the LF-EMF kill a part of E. coli exposed in the river water with the large magnetic field and long-time exposure.

  13. Scientific Programs and Funding Opportunities at the National Institute of Biomedical Imaging and Bioengineering

    Science.gov (United States)

    Baird, Richard

    2006-03-01

    The mission of the National Institute of Biomedical Imaging and Bioengineering (NIBIB) is to improve human health by promoting the development and translation of emerging technologies in biomedical imaging and bioengineering. To this end, NIBIB supports a coordinated agenda of research programs in advanced imaging technologies and engineering methods that enable fundamental biomedical discoveries across a broad spectrum of biological processes, disorders, and diseases and have significant potential for direct medical application. These research programs dramatically advance the Nation's healthcare by improving the detection, management and, ultimately, the prevention of disease. The research promoted and supported by NIBIB also is strongly synergistic with other NIH Institutes and Centers as well as across government agencies. This presentation will provide an overview of the scientific programs and funding opportunities supported by NIBIB, highlighting those that are of particular important to the field of medical physics.

  14. Bioengineering Embryonic Stem Cell Microenvironments for the Study of Breast Cancer

    Directory of Open Access Journals (Sweden)

    Yubing Xie

    2011-11-01

    Full Text Available Breast cancer is the most prevalent disease amongst women worldwide and metastasis is the main cause of death due to breast cancer. Metastatic breast cancer cells and embryonic stem (ES cells display similar characteristics. However, unlike metastatic breast cancer cells, ES cells are nonmalignant. Furthermore, embryonic microenvironments have the potential to convert metastatic breast cancer cells into a less invasive phenotype. The creation of in vitro embryonic microenvironments will enable better understanding of ES cell-breast cancer cell interactions, help elucidate tumorigenesis, and lead to the restriction of breast cancer metastasis. In this article, we will present the characteristics of breast cancer cells and ES cells as well as their microenvironments, importance of embryonic microenvironments in inhibiting tumorigenesis, convergence of tumorigenic and embryonic signaling pathways, and state of the art in bioengineering embryonic microenvironments for breast cancer research. Additionally, the potential application of bioengineered embryonic microenvironments for the prevention and treatment of invasive breast cancer will be discussed.

  15. A Bioengineered Living Cell Construct Activates an Acute Wound Healing Response in Venous Leg Ulcers

    OpenAIRE

    Stone, Rivka C.; Stojadinovic, Olivera; Rosa, Ashley M.; Ramirez, Horacio A.; Badiavas, Evangelos; Blumenberg, Miroslav; Tomic-Canic, Marjana

    2017-01-01

    Chronic non-healing venous leg ulcers (VLUs) are widespread and debilitating, with high morbidity and associated costs; approximately $15 billion is spent annually on the care of VLUs in the US. Despite this, there is a paucity of treatments for VLUs, due to the lack of pathophysiologic insight into ulcer development as well as the lack of knowledge regarding biologic actions of existing VLU-targeted therapies. The bioengineered bilayered living cellular construct (BLCC) skin substitute is an...

  16. Restoration of active gully systems following the implementation of bioengineering techniques.

    Science.gov (United States)

    Borja, Pablo; Vanacker, Veerle; Govers, Gerard

    2015-04-01

    Intensive land use in the central parts of the Andean basin has led to widespread land degradation. The formation of badlands dates back from the 1950s and 1960s. Several studies indicate that human activities have accelerated mountain erosion rates by up to 100 times. In this study, we have evaluated the effects of bio-engineering works aiming to stabilize degraded catchments. Five micro-catchments (0.2 up to 5 ha) have been selected within a 3 km2 area in the lower part of the Loreto catchment (Southern Ecuadorian Andes). The five micro-catchments differ in vegetation cover and implementation of bio-engineering works. The experimental design consisted of three micro-catchments: (1) DI with conservation works, (2) DF with reforestation by Eucalyptus sp and (3) DT with no conservation works. Two micro-catchments have been monitored in an agricultural area: with (AI) and without (AT) bio-engineering works in the active gullies. Small checkdams were constructed in the gully floors of two of the micro-catchments in the badland area (DI) and the agricultural area (AI). The checkdams are made of wood and tires. Water flow has been measured in every micro-catchment, while sediment traps were constructed to monitor sediment transport. Results show that bio-engineering techniques are effective to stabilize active gullies. Deposition of sediments in manmade dams is strongly dependent on previous rainfall events, as well as gully channel slope, and its vegetation cover. From the experimental data, an I30 max threshold value was determined. Above this threshold value, all micro-catchments are actively contributing sediment to the main river system. The checkdams built with wood and tires have an efficiency of 70%, and were shown to be very effective to stabilize active gullies in bad lands through significant reduction (about 62%) of the amount of sediment exported from the micro-catchments. Key words: degraded soils, erosion, sediment, restoration, reforestation

  17. Bioengineered Hydrogel to Inhibit Post-Traumatic Central Nervous System Scarring

    Science.gov (United States)

    2016-10-01

    AWARD NUMBER: W81XWH-14-1-0586 TITLE: Bioengineered Hydrogel to Inhibit Post-Traumatic Central Nervous System Scarring PRINCIPAL...Hydrogel to Inhibit Post-Traumatic Central Nervous System Scarring 5a. CONTRACT NUMBER W81XWH-14-1-0586 5b. GRANT NUMBER W81XWH- 14-1-0586 5c...barriers that prevent the optimal delivery of biologics and cells to the injured nervous system . A significant problem is the formation of scar tissue

  18. A translational bioengineering course provides substantial gains in civic scientific literacy.

    Science.gov (United States)

    Richards-Kortum, Rebecca; Buckley, Deanna; Schwarz, Richard A; Atkinson, E Neely; Follen, Michele

    2007-08-01

    A growing number of essential consumer choices and public policy issues require a basic level of scientific literacy. Recent studies suggest as many as three-quarters of adults are unable to read and understand news accounts of scientific advances and controversies. In response to this challenge, a new course for non-science majors, Bioengineering and World Health, was designed to improve biomedical literacy. The goal of this study was to compare scientific literacy of students enrolled in the course to that of two groups of students who had not taken the course; the first control group included students majoring in Biomedical Engineering (BME), the second included those majoring in Liberal Arts or Natural Sciences. Small group interviews in which students discussed science news accounts from the popular press were used to assess scientific literacy. Students in Bioengineering and World Health showed increasing scientific literacy throughout the course. At the conclusion of Bioengineering and World Health, the mean scientific literacy of students in the course was significantly higher than that in both control groups. Students were stratified by the number of semester credit hours completed in science, math, engineering and technology (SME&T) courses. Regardless of number of SME&T hours completed, the mean scientific literacy of students completing Bioengineering and World Health was equivalent to that of BME majors who had completed more than 60 semester credit hours of SME&T coursework, suggesting that a single introductory course can significantly influence scientific literacy as measured by participant's ability to discuss medical innovations from a common news source.

  19. Update on Renal Replacement Therapy: Implantable Artificial Devices and Bioengineered Organs.

    Science.gov (United States)

    Attanasio, Chiara; Latancia, Marcela T; Otterbein, Leo E; Netti, Paolo A

    2016-08-01

    Recent advances in the fields of artificial organs and regenerative medicine are now joining forces in the areas of organ transplantation and bioengineering to solve continued challenges for patients with end-stage renal disease. The waiting lists for those needing a transplant continue to exceed demand. Dialysis, while effective, brings different challenges, including quality of life and susceptibility to infection. Unfortunately, the majority of research outputs are far from delivering satisfactory solutions. Current efforts are focused on providing a self-standing device able to recapitulate kidney function. In this review, we focus on two remarkable innovations that may offer significant clinical impact in the field of renal replacement therapy: the implantable artificial renal assist device (RAD) and the transplantable bioengineered kidney. The artificial RAD strategy utilizes micromachining techniques to fabricate a biohybrid system able to mimic renal morphology and function. The current trend in kidney bioengineering exploits the structure of the native organ to produce a kidney that is ready to be transplanted. Although these two systems stem from different technological approaches, they are both designed to be implantable, long lasting, and free standing to allow patients with kidney failure to be autonomous. However, for both of them, there are relevant issues that must be addressed before translation into clinical use and these are discussed in this review.

  20. Assessing the National Bioengineered Food Disclosure Standard of 2016: Can Americans Access Electronic Disclosure Information?

    Directory of Open Access Journals (Sweden)

    Craig F. Berning

    2017-05-01

    Full Text Available The debate as to whether to require mandatory labeling of genetically modified organism (GMO foods was partially settled on 29 July 2016, when President Obama signed the National Bioengineered Food Disclosure Standard into public law. In contrast to precipitating legislation passed by the State of Vermont that required disclosure of GMO ingredients on food shelves or food packages, the superseding National Standard allows firms to disclose bioengineered ingredients to consumers via symbols, electronic or digital links, or phone numbers, and further requires a study assessing the ability of consumers to access disclosure information by these means. This communication analyzes survey responses from 525 adults to investigate whether U.S. consumers are able to obtain information as per the disclosure methods allowed in the Federal legislation. The survey probes deeper to investigate consumer perceptions of genetically modified organisms and whether consumers would use the tools available to access disclosure about bioengineered ingredients. Findings from the survey show that 93.8% of respondents have the ability to access information via the disclosure methods permitted. Those in the lowest income group, and from the oldest age group are least likely to have such access. This provides the United State Department of Agriculture with information relevant to how they can implement the law and highlights particular demographic segments that may require additional attention to ensure the disclosed information is universally accessible.

  1. Seismic response monitoring of the Arno river masonry embankment during the conservation works after the Lungarno Torrigiani riverbank landslide (Florence - May 25, 2016)

    Science.gov (United States)

    Lotti, Alessia; Pazzi, Veronica; Chiara, Paolo; Lombardi, Luca; Nocentini, Massimiliano; Casagli, Nicola

    2017-04-01

    Geohazards are the most relevant processes that can damage or increase the risk of human beings, properties, critical and transport infrastructures, and environment itself. They also could involve the interruption of human activities. The concepts of disaster risk reduction and disaster risk management involve the development, improvement, and application of policies, strategies, and practices to minimize disaster risks throughout society. Since 1972 (UNESCO Convention) the identification, protection, and preservation of cultural and natural heritage has been recognized to be of outstanding value to humanity, and a key resource to build resilient societies. Nevertheless, world architectural wealth is accumulating damages and heavy losses because of both materials deterioration and exceptional natural or man-made events. The "health" of buildings/structures/infrastructures may be evaluate by its deterioration or damage level. Thus, structure dynamic characterization and microtremor analysis are considered powerful techniques, even thought seismic noise techniques in densely populated area are hardly to carry out because of the background noise due to the human activities. A wide bibliography about buildings/structures/infrastructures seismic dynamic characterization is counterposed to a missing one about their seismic response during conservation/safety works, even thought the seismic vibration monitoring (SVM) is widely used. On May 25, 2016 a riverbank landslide seriously damaged a portion roughly 100 m long of the Lungarno Torrigiani historical masonry embankment wall (left river bank of the Florence urban stretch of the Arno river, between Ponte alle Grazie e Ponte Vecchio). The street next to the embankment wall collapsed, and the earth fill material was fully retained by the embankment wall that did not collapse but seriously deformed towards the Arno river, fracturing itself in three main areas (a cusp roughly in the middle of the damaged wall, where is also

  2. Preliminary analysis on mowing and harvesting grass along riverbanks for the supply of anaerobic digestion plants in north-eastern Italy

    Directory of Open Access Journals (Sweden)

    Davide Boscaro

    2015-10-01

    Full Text Available The increasing demand of vegetal biomass for biogas production is causing competition with food production. To reduce this problem and to provide new opportunities it is necessary to take into consideration different kinds of vegetable biomass that are more sustainable. Grass from the maintenance of non-cultivated areas such as riverbanks has not yet been fully studied as a potential biomass for biogas production. Although grass has lower methane potential, it could be interesting because it does not compete with food production. However, there is a lack of appropriate technologies and working system adapted to these areas. In this paper, different systems that could be available for the mowing and harvesting of grass along riverbanks have been preliminarily assessed through the evaluation of the field capacity, labour requirement, economic and energy aspects. The splitting of the cutting and harvesting phases into operations with different machinery seems to be the best system for handling this biomass. However, these solutions have to take into consideration the presence of obstacles or accessibility problems in the harvesting areas that could limit the operational feasibility and subsequent correct sizing.

  3. Potential of Ranunculus acris L. for biomonitoring trace element contamination of riverbank soils: photosystem II activity and phenotypic responses for two soil series.

    Science.gov (United States)

    Marchand, Lilian; Lamy, Pierre; Bert, Valerie; Quintela-Sabaris, Celestino; Mench, Michel

    2016-02-01

    Foliar ionome, photosystem II activity, and leaf growth parameters of Ranunculus acris L., a potential biomonitor of trace element (TE) contamination and phytoavailability, were assessed using two riverbank soil series. R. acris was cultivated on two potted soil series obtained by mixing a TE (Cd, Cu, Pb, and Zn)-contaminated technosol with either an uncontaminated sandy riverbank soil (A) or a silty clay one slightly contaminated by TE (B). Trace elements concentrations in the soil-pore water and the leaves, leaf dry weight (DW) yield, total leaf area (TLA), specific leaf area (SLA), and photosystem II activity were measured for both soil series after a 50-day growth period. As soil contamination increased, changes in soluble TE concentrations depended on soil texture. Increase in total soil TE did not affect the leaf DW yield, the TLA, the SLA, and the photosystem II activity of R. acris over the 50-day exposure. The foliar ionome did not reflect the total and soluble TE concentrations in both soil series. Foliar ionome of R. acris was only effective to biomonitor total and soluble soil Na concentrations in both soil series and total and soluble soil Mo concentrations in the soil series B.

  4. Monitoring Inter- and Intra-Seasonal Dynamics of Rapidly Degrading Ice-Rich Permafrost Riverbanks in the Lena Delta with TerraSAR-X Time Series

    Directory of Open Access Journals (Sweden)

    Samuel Stettner

    2017-12-01

    Full Text Available Arctic warming is leading to substantial changes to permafrost including rapid degradation of ice and ice-rich coasts and riverbanks. In this study, we present and evaluate a high spatiotemporal resolution three-year time series of X-Band microwave satellite data from the TerraSAR-X (TSX satellite to quantify cliff-top erosion (CTE of an ice-rich permafrost riverbank in the central Lena Delta. We apply a threshold on TSX backscatter images and automatically extract cliff-top lines to derive intra- and inter-annual CTE. In order to examine the drivers of erosion we statistically compare CTE with climatic baseline data using linear mixed models and analysis of variance (ANOVA. Our evaluation of TSX-derived CTE against annual optical-derived CTE and seasonal in situ measurements showed good agreement between all three datasets. We observed continuous erosion from June to September in 2014 and 2015 with no significant seasonality across the thawing season. We found the highest net annual cliff-top erosion of 6.9 m in 2014, in accordance with above-average mean temperatures and thawing degree days as well as low precipitation. We found high net annual erosion and erosion variability in 2015 associated with moderate mean temperatures but above average precipitation. According to linear mixed models, climate parameters alone could not explain intra-seasonal erosional patterns and additional factors such as ground ice content likely drive the observed erosion. Finally, mean backscatter intensity on the cliff surface decreased from −5.29 to −6.69 dB from 2013 to 2015, respectively, likely resulting from changes in surface geometry and properties that could be connected to partial slope stabilization. Overall, we conclude that X-Band backscatter time series can successfully be used to complement optical remote sensing and in situ monitoring of rapid tundra permafrost erosion at riverbanks and coasts by reliably providing information about intra

  5. Assessing the transport and fate of bioengineered microorganisms in the environment

    International Nuclear Information System (INIS)

    Barnthouse, L.W.; Palumbo, A.V.

    1985-01-01

    We review the methods currently available for quantifying the transport and fate of microbes in atmospheric and aqueous media and assess their adequacy for purposes of risk assessment. We review the literature on transport and fate of microorganisms, including studies of: (1) pathways of migration, (2) the survival of microorganisms during transport and fate. In addition, we review the transport and fate models that have been used in environmental risk assessments for radionuclides and toxic chemicals and evaluate their applicability to the problem of assessing environmental risks of bioengineered microorganisms

  6. Profile of prospective bioengineering students at National University of San Juan

    International Nuclear Information System (INIS)

    Lopez, N; Puzzella, A; Zabala, A; Demartini, H; Alborch, A; Cabrera, L

    2007-01-01

    The low percentage of students (43 % of applicants) that passed the entrance exams for the bioengineering career at the National University of San Juan in 2007, plus the historical situation of desertion in first year (about 50%), motivated the application of a diagnostic test to prospective students of this career. The aim of this test was to obtain information about the competences acquired by students to solve problems in different contexts using basic mathematical tools, reading comprehension skills to understand texts, graphs and tables. Although this test was sat by the entire population of applicants of the current school year, only the results belonging to bioengineering students are the ones presented for the purpose of this work. However, students of other disciplines of the school of engineering also have similar problems. From the analysis of the answers to the different items, it can be observed that there are serious difficulties in the development of basic capacities to successfully take the courses of this career

  7. Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering.

    Science.gov (United States)

    Bernard, Aurélien; He, Zhiguo; Forest, Fabien; Gauthier, Anne-Sophie; Peocʼh, Michel; Dumollard, Jean-Marc; Acquart, Sophie; Montard, Romain; Delbosc, Bernard; Gain, Philippe; Thuret, Gilles

    2015-02-01

    To assess the feasibility of cutting multiple thin stromal lamellae in human donor corneas using a commercial femtosecond laser (FSL) to provide cell carriers for future endothelial graft bioengineering. Eight edematous organ-cultured corneas not suitable for grafting for endothelial reasons were mounted on a Ziemer anterior chamber and cut with a Z6 FSL with 6 successive parallel cuts, from depth to surface. Target thickness of each lamella ranged from 100 to 150 μm depending on initial corneal thickness. Thickness was measured using anterior segment optical coherence tomography before and after cutting on mounted corneas, and on each stromal lamella after detachment. Scanning electron microscopy observation was performed on 4 lamellae and histological cross sections on 1 cornea before detachment. A median of 5 (minimum 3, maximum 7) lamellae was obtained per cornea. All lamellae still attached were the most posterior ones, suggesting that FSL was less efficient because of light scattering by edematous stroma. Cut precision and postdetachment swelling were correlated with anterior-posterior position within the cornea. Median lamella thickness was 127 μm (56-222 μm) before detachment and 196 μm (80-304 μm) after detachment. Surface state was consistent with previously reported FSL lamellar cuts during Descemet stripping automated endothelial keratoplasty. Up to 7 thin lamellae can be cut in stored corneas with an FSL. This method, once optimized primarily by using deswelled, more transparent corneas, could prove effective for recycling unsuitable donor corneas in corneal bioengineering processes.

  8. Bioengineered Systems and Designer Matrices That Recapitulate the Intestinal Stem Cell Niche

    Directory of Open Access Journals (Sweden)

    Yuli Wang

    2018-01-01

    Full Text Available The relationship between intestinal stem cells (ISCs and the surrounding niche environment is complex and dynamic. Key factors localized at the base of the crypt are necessary to promote ISC self-renewal and proliferation, to ultimately provide a constant stream of differentiated cells to maintain the epithelial barrier. These factors diminish as epithelial cells divide, migrate away from the crypt base, differentiate into the postmitotic lineages, and end their life span in approximately 7 days when they are sloughed into the intestinal lumen. To facilitate the rapid and complex physiology of ISC-driven epithelial renewal, in vivo gradients of growth factors, extracellular matrix, bacterial products, gases, and stiffness are formed along the crypt-villus axis. New bioengineered tools and platforms are available to recapitulate various gradients and support the stereotypical cellular responses associated with these gradients. Many of these technologies have been paired with primary small intestinal and colonic epithelial cells to re-create select aspects of normal physiology or disease states. These biomimetic platforms are becoming increasingly sophisticated with the rapid discovery of new niche factors and gradients. These advancements are contributing to the development of high-fidelity tissue constructs for basic science applications, drug screening, and personalized medicine applications. Here, we discuss the direct and indirect evidence for many of the important gradients found in vivo and their successful application to date in bioengineered in vitro models, including organ-on-chip and microfluidic culture devices.

  9. Profile of prospective bioengineering students at National University of San Juan

    Science.gov (United States)

    Lopez, N.; Puzzella, A.; Zabala, A.; Demartini, H.; Alborch, A.; Cabrera, L.

    2007-11-01

    The low percentage of students (43 % of applicants) that passed the entrance exams for the bioengineering career at the National University of San Juan in 2007, plus the historical situation of desertion in first year (about 50%), motivated the application of a diagnostic test to prospective students of this career. The aim of this test was to obtain information about the competences acquired by students to solve problems in different contexts using basic mathematical tools, reading comprehension skills to understand texts, graphs and tables. Although this test was sat by the entire population of applicants of the current school year, only the results belonging to bioengineering students are the ones presented for the purpose of this work. However, students of other disciplines of the school of engineering also have similar problems. From the analysis of the answers to the different items, it can be observed that there are serious difficulties in the development of basic capacities to successfully take the courses of this career.

  10. Profile of prospective bioengineering students at National University of San Juan

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, N [School of Engineering. National University of San Juan. Av. San MartIn 1109 (Oeste). CP 5400. San Juan (Argentina); Puzzella, A [School of Philosophy, Humanities and Arts. National University of San Juan. I de la Roza 230 (Oeste). CP 5400. San Juan (Argentina); Zabala, A [School of Engineering. National University of San Juan. Av. San MartIn 1109 (Oeste). CP 5400. San Juan (Argentina); Demartini, H [School of Engineering. National University of San Juan. Av. San MartIn 1109 (Oeste). CP 5400. San Juan (Argentina); Alborch, A [School of Philosophy, Humanities and Arts. National University of San Juan. I de la Roza 230 (Oeste). CP 5400. San Juan (Argentina); Cabrera, L [' Col. Central Universitario Dr. M. Moreno' Secondary School. National University of San Juan. I de la Roza 230 (Oeste). CP 5400. San Juan (Argentina)

    2007-11-15

    The low percentage of students (43 % of applicants) that passed the entrance exams for the bioengineering career at the National University of San Juan in 2007, plus the historical situation of desertion in first year (about 50%), motivated the application of a diagnostic test to prospective students of this career. The aim of this test was to obtain information about the competences acquired by students to solve problems in different contexts using basic mathematical tools, reading comprehension skills to understand texts, graphs and tables. Although this test was sat by the entire population of applicants of the current school year, only the results belonging to bioengineering students are the ones presented for the purpose of this work. However, students of other disciplines of the school of engineering also have similar problems. From the analysis of the answers to the different items, it can be observed that there are serious difficulties in the development of basic capacities to successfully take the courses of this career.

  11. Using biomimetic cell wall models to identify new plant lignin bioengineering targets for improving forage and biomass utilization

    Science.gov (United States)

    Bioengineering of lignin to contain atypical components derived from other metabolic pathways is increasingly being pursued to custom design lignified cell walls that are inherently more digestible by livestock or more easily pretreated and saccharified for biofuel production. Because plants produce...

  12. Identifying new lignin bioengineering targets for improving biomass and forage utilization: a review of biomimetic studies with maize cell walls

    Science.gov (United States)

    Bioengineering of lignin to contain atypical components derived from other metabolic pathways is increasingly being pursued to custom design lignified cell walls that are more readily pretreated and saccharified for biofuel production or easily digested by livestock. Because plants produce such a di...

  13. Biomimetic cell wall model studies to identify new lignin bioengineering targets for improving biomass susceptibility to pretreatment and enzymatic saccharification

    Science.gov (United States)

    Increasingly, bioengineering of lignin to contain atypical building blocks from other metabolic pathways is being pursued to custom-design lignin that is easier to remove by chemical pretreatments and less inhibitory toward polysaccharide saccharification. Because plants produce such a diverse array...

  14. Development of a compact permanent magnet helicon plasma source for ion beam bioengineering

    Energy Technology Data Exchange (ETDEWEB)

    Kerdtongmee, P.; Srinoum, D.; Nisoa, M. [Plasma Technology for Agricultural Applications Research Laboratory, School of Science, Walailak University, Nakhon Si Thammarat 80161 (Thailand); ThEP Center, CHE, 328 Si Ayutthaya Rd., Bangkok 10400 (Thailand)

    2011-10-15

    A compact helicon plasma source was developed as a millimeter-sized ion source for ion beam bioengineering. By employing a stacked arrangement of annular-shaped permanent magnets, a uniform axial magnetic flux density up to 2.8 kG was obtained. A cost effective 118 MHz RF generator was built for adjusting forward output power from 0 to 40 W. The load impedance and matching network were then analyzed. A single loop antenna and circuit matching elements were placed on a compact printed circuit board for 50 {Omega} impedance matching. A plasma density up to 1.1 x 10{sup 12} cm{sup -3} in the 10 mm diameter tube under the magnetic flux density was achieved with 35 W applied RF power.

  15. Development of a compact permanent magnet helicon plasma source for ion beam bioengineering.

    Science.gov (United States)

    Kerdtongmee, P; Srinoum, D; Nisoa, M

    2011-10-01

    A compact helicon plasma source was developed as a millimeter-sized ion source for ion beam bioengineering. By employing a stacked arrangement of annular-shaped permanent magnets, a uniform axial magnetic flux density up to 2.8 kG was obtained. A cost effective 118 MHz RF generator was built for adjusting forward output power from 0 to 40 W. The load impedance and matching network were then analyzed. A single loop antenna and circuit matching elements were placed on a compact printed circuit board for 50 Ω impedance matching. A plasma density up to 1.1 × 10(12) cm(-3) in the 10 mm diameter tube under the magnetic flux density was achieved with 35 W applied RF power.

  16. Bioengineering aspects of inorganic carbon supply to mass algal cultures. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Goldman, J.C.

    1980-06-01

    The work included in this report is part of an ongoing study (currently funded by the Solar Energy Research Institute - Subcontract No. XR-9-8144-1) on the inorganic carbon requirements of microalgae under mass culture conditions and covers the period June 1, 1978 through May 31, 1979. It is divided into two parts appended herein. The first part is a literature review on the inorganic carbon chemical system in relation to algal growth requirements, and the second part deals with the kinetics of inorganic carbon-limited growth of two freshwater chlorophytes including the effect of carbon limitation on cellular chemical composition. Additional experiment research covered under this contract was reported in the Proceedings of the 3rd Annual Biomass Energy Systems Conferences, pp. 25-32, Bioengineering aspects of inorganic carbon supply to mass algal cultures. Report No. SERI/TP-33-285.

  17. An innovative bio-engineering retaining structure for supporting unstable soil

    Directory of Open Access Journals (Sweden)

    Gianluca Bella

    2017-04-01

    Full Text Available The paper presents a new prefabricated bio-engineering structure for the support of unstable soil. This prefabricated structure is made of a steel frame which is completely filled with soil and a face made of tree trunks among which scions or autochthonous bushes are planted. Due to the difficulties in interpreting the complex interaction between soil and structure during the installation and lifetime, an in situ test was carried out in order to evaluate the state of stress in the steel frame and to understand the global behavior of the structure under service loads. On the basis of the obtained results, a procedure for checking the structure safety was proposed and discussed. An easy design method was developed during the research. Moreover, the use of this type of prefabricated structure shows several advantages, such as good performances in terms of stabilizing effects, and easy assembly and transport.

  18. Validity and reliability of an instrument for assessing case analyses in bioengineering ethics education.

    Science.gov (United States)

    Goldin, Ilya M; Pinkus, Rosa Lynn; Ashley, Kevin

    2015-06-01

    Assessment in ethics education faces a challenge. From the perspectives of teachers, students, and third-party evaluators like the Accreditation Board for Engineering and Technology and the National Institutes of Health, assessment of student performance is essential. Because of the complexity of ethical case analysis, however, it is difficult to formulate assessment criteria, and to recognize when students fulfill them. Improvement in students' moral reasoning skills can serve as the focus of assessment. In previous work, Rosa Lynn Pinkus and Claire Gloeckner developed a novel instrument for assessing moral reasoning skills in bioengineering ethics. In this paper, we compare that approach to existing assessment techniques, and evaluate its validity and reliability. We find that it is sensitive to knowledge gain and that independent coders agree on how to apply it.

  19. Bioengineering a non-genotoxic vector for genetic modification of mesenchymal stem cells.

    Science.gov (United States)

    Chen, Xuguang; Nomani, Alireza; Patel, Niket; Nouri, Faranak S; Hatefi, Arash

    2018-01-01

    Vectors used for stem cell transfection must be non-genotoxic, in addition to possessing high efficiency, because they could potentially transform normal stem cells into cancer-initiating cells. The objective of this research was to bioengineer an efficient vector that can be used for genetic modification of stem cells without any negative somatic or genetic impact. Two types of multifunctional vectors, namely targeted and non-targeted were genetically engineered and purified from E. coli. The targeted vectors were designed to enter stem cells via overexpressed receptors. The non-targeted vectors were equipped with MPG and Pep1 cell penetrating peptides. A series of commercial synthetic non-viral vectors and an adenoviral vector were used as controls. All vectors were evaluated for their efficiency and impact on metabolic activity, cell membrane integrity, chromosomal aberrations (micronuclei formation), gene dysregulation, and differentiation ability of stem cells. The results of this study showed that the bioengineered vector utilizing VEGFR-1 receptors for cellular entry could transfect mesenchymal stem cells with high efficiency without inducing genotoxicity, negative impact on gene function, or ability to differentiate. Overall, the vectors that utilized receptors as ports for cellular entry (viral and non-viral) showed considerably better somato- and genosafety profiles in comparison to those that entered through electrostatic interaction with cellular membrane. The genetically engineered vector in this study demonstrated that it can be safely and efficiently used to genetically modify stem cells with potential applications in tissue engineering and cancer therapy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Root reinforcement and slope bioengineering stabilization by Spanish Broom (Spartium junceum L.

    Directory of Open Access Journals (Sweden)

    F. Giadrossich

    2009-09-01

    Full Text Available The present paper deals with the root system's characteristics of Spanish Broom (Spartium junceum L., a species whose capacity for adaptating and resisting to drought is worth investigating. In particular, the aims of the study were 1 to investigate the plant's bio-mechanical aspects and 2 to verify whether root reinforcement and the field rooting ability of stem cuttings enhance its potential for use in slope stabilization and soil bio-engineering techniques, particularly in the Mediterranean areas. Single root specimens were sampled and tested for tensile strength, obtaining classic tensile strength-diameter relationships. Analysis were performed on the root systems in order to assess root density distribution. The Root Area Ratio (RAR was analyzed by taking both direct and indirect measurements, the latter relying on image processing. The data obtained were used to analyze the stability of an artificial slope (landfill and the root reinforcement. The measurement and calculation of mean root number, mean root diameter, RAR, root cohesion and Factor of safety are presented in order to distinguish the effect of plant origin and propagation. Furthermore, tests were performed to assess the possibility of agamic propagation (survival rate of root-ball endowed plants, rooting from stem cuttings. These tests confirmed that agamic propagation is difficult, even though roots were produced from some buried stems, and for practical purposes it has been ruled out. Our results show that Spanish Broom has good bio-mechanical characteristics with regard to slope stabilization, even in critical pedoclimatic conditions and where inclinations are quite steep, and it is effective on soil depths up to about 50 cm, in agreement with other studies on Mediterranean species. It is effective in slope stabilization, but less suitable for soil bio-engineering or for triggering natural plant succession.

  1. Elemental composition study of heavy metal (Ni, Cu, Zn) in riverbank soil by electrokinetic-assisted phyto remediation using XRF and SEM/EDX

    International Nuclear Information System (INIS)

    Suhailly Jamari; Zaidi Embong; Zaidi Embong; Ismail Bakar

    2013-01-01

    Full-text: Electrokinetic (EK)-assisted phyto remediation is one of the methods that have a big potential in enhancing the ability of plant uptake in soils remediation process. This research was conducted to investigate the difference in elemental composition concentration of riverbank soil and the change of pH between pre- and post-phyto remediation under the following condition: 1) control or as-receive sample; 2) Dieffenbachia spp plant with EK system (a pair of EK electrodes connected to a direct current (DC) power supply). After the electrodes were connected to a magnitude of 6 V/ cm -1 electric field for 4 hours/ day, the soil and plant samples were analyzed using and X-ray Fluorescence Spectrometer (XRF) and Scanning Electron Microscope/ Energy Dispersive X-ray Spectroscopy (SEM/ EDX). The SEM/EDX analysis showed that concentration of elemental composition (Ni, Cu and Zn) in post-phyto remediation plant powder samples had increase while elemental concentrations in the post-hydrotreatment soil samples were decreased. XRF analysis presented a variation in soil elemental composition concentration from anode to cathode where the concentration near anode region increased while decreased near the cathode region. A significant changes in soil pH were obtained where the soil pH increase in cathode region while decrease in anode region. The results reveal that the assistance of EK in phyto remediation process has increase the efficiency of plant uptake. (author)

  2. Elemental composition study of heavy metal (Ni, Cu, Zn) in riverbank soil by electrokinetic-assisted phytoremediation using XRF and SEM/EDX

    International Nuclear Information System (INIS)

    Jamari, Suhailly; Embong, Zaidi; Bakar, Ismail

    2014-01-01

    Electrokinetic (EK)-assisted phytoremediation is one of the methods that have a big potential in enhancing the ability of plant uptake in soils remediation process. This research was conducted to investigate the difference in elemental composition concentration of riverbank soil and the change of pH between pre- and post-phytoremediation under the following condition: 1) control or as-receive sample; 2) Dieffenbachia spp plant with EK system (a pair of EK electrodes connected to a direct current (DC) power supply). After the electrodes were connected to a magnitude of 6V/cm −1 electric field for 4 hours/day, the soil and plant samples were analyzed using and X-ray Fluorescence Spectrometer (XRF) and Scanning Electron Microscope / Energy Dispersive X-ray Spectroscopy (SEM/EDX). The SEM/EDX analysis showed that concentration of elemental composition (Ni, Cu and Zn) in post-phytoremediation plant powder samples had increase while elemental concentrations in the post-phytoremediation soil samples were decreased. XRF analysis presented a variation in soil elemental composition concentration from anode to cathode where the concentration near anode region increased while decreased near the cathode region. A significant changes in soil pH were obtained where the soil pH increase in cathode region while decrease in anode region. The results reveal that the assistance of EK in phytoremediation process has increase the efficiency of plant uptake

  3. Elemental composition study of heavy metal (Ni, Cu, Zn) in riverbank soil by electrokinetic-assisted phytoremediation using XRF and SEM/EDX

    Energy Technology Data Exchange (ETDEWEB)

    Jamari, Suhailly [Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia (UTHM) (Malaysia); Embong, Zaidi [Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia (UTHM) and Research Center for Soft Soils (RECESS), Office of Research, Innovation, Commercialization and Consultancy Management - ORRIC, Universiti Tun Hussein (Malaysia); Bakar, Ismail [Research Center for Soft Soils (RECESS), Office of Research, Innovation, Commercialization and Consultancy Management (ORRIC), Universiti Tun Hussein Onn Malaysia -UTHM, 86400 Batu Pahat, Johor (Malaysia)

    2014-02-12

    Electrokinetic (EK)-assisted phytoremediation is one of the methods that have a big potential in enhancing the ability of plant uptake in soils remediation process. This research was conducted to investigate the difference in elemental composition concentration of riverbank soil and the change of pH between pre- and post-phytoremediation under the following condition: 1) control or as-receive sample; 2) Dieffenbachia spp plant with EK system (a pair of EK electrodes connected to a direct current (DC) power supply). After the electrodes were connected to a magnitude of 6V/cm{sup −1} electric field for 4 hours/day, the soil and plant samples were analyzed using and X-ray Fluorescence Spectrometer (XRF) and Scanning Electron Microscope / Energy Dispersive X-ray Spectroscopy (SEM/EDX). The SEM/EDX analysis showed that concentration of elemental composition (Ni, Cu and Zn) in post-phytoremediation plant powder samples had increase while elemental concentrations in the post-phytoremediation soil samples were decreased. XRF analysis presented a variation in soil elemental composition concentration from anode to cathode where the concentration near anode region increased while decreased near the cathode region. A significant changes in soil pH were obtained where the soil pH increase in cathode region while decrease in anode region. The results reveal that the assistance of EK in phytoremediation process has increase the efficiency of plant uptake.

  4. Complete genome sequence of cyanobacterium Nostoc sp. NIES-3756, a potentially useful strain for phytochrome-based bioengineering.

    Science.gov (United States)

    Hirose, Yuu; Fujisawa, Takatomo; Ohtsubo, Yoshiyuki; Katayama, Mitsunori; Misawa, Naomi; Wakazuki, Sachiko; Shimura, Yohei; Nakamura, Yasukazu; Kawachi, Masanobu; Yoshikawa, Hirofumi; Eki, Toshihiko; Kanesaki, Yu

    2016-01-20

    To explore the diverse photoreceptors of cyanobacteria, we isolated Nostoc sp. strain NIES-3756 from soil at Mimomi-Park, Chiba, Japan, and determined its complete genome sequence. The Genome consists of one chromosome and two plasmids (total 6,987,571 bp containing no gaps). The NIES-3756 strain carries 7 phytochrome and 12 cyanobacteriochrome genes, which will facilitate the studies of phytochrome-based bioengineering. Copyright © 2015. Published by Elsevier B.V.

  5. Bioengineering methods employed in the study of wound healing of sulphur mustard burns.

    Science.gov (United States)

    Graham, John S; Schomacker, Kevin T; Glatter, Robert D; Briscoe, Crystal M; Braue, Ernest H; Squibb, Katherine S

    2002-02-01

    Sulphur mustard (SM) is a potent incapacitating chemical warfare agent that remains a threat to war fighters and civilians worldwide. SM lesions may require weeks or months to heal, depending upon their severity. This study was undertaken to find a treatment regimen that promotes speedier healing of deep cutaneous SM burns in a weanling pig model. The principal objective of the study was to compare four treatment regimens and establish which achieved the shortest healing time. Twelve Yorkshire Cross weanling pigs were exposed to SM liquid for 2h, generating six large deep dermal/full thickness burns on the ventrum of each animal. Three additional animals served as sham-exposed controls. Surgical intervention occurred at 48 h postexposure. Treatments included: (i) full-thickness debridement of the burns with a computer controlled, raster scanned continuous wave CO2 laser followed by autologous split-thickness skin grafting; (ii) full-thickness sharp surgical tangential excision followed by skin grafting, the 'Gold Standard' used in human deep dermal/full-thickness thermal burns management; (iii) partial-thickness laser ablation with no grafting; and (iv) partial-thickness sharp surgical excision with no grafting. Several non-invasive bioengineering methods were used to monitor the progress of wound healing throughout a 36-day healing period: reflectance colourimetry, evaporimetry, laser Doppler perfusion imaging and ballistometry. Bioengineering methods indicated that laser debridement followed by autologous split-thickness skin grafting was as efficacious in improving the wound healing of deep SM burns in weanling swine as the 'Gold Standard.' Regardless of the method of debridement, barrier function, skin colour and mechanical properties returned to near-normal levels within 15 days of treatment in the grafted sites. Regardless of surgical approach, blood flux levels remained approximately 50-60% of normal tissue throughout the 36-day postsurgical observation

  6. Horizon Scanning: How Will Metabolomics Applications Transform Food Science, Bioengineering, and Medical Innovation in the Current Era of Foodomics?

    Science.gov (United States)

    Bayram, Mustafa; Gökırmaklı, Çağlar

    2018-03-01

    Food and engineering sciences have tended to neglect the importance of human nutrition sciences and clinical study of new molecules discovered by food engineering community, and vice versa. Yet, the value of systems thinking and use of omics technologies in food engineering are rapidly emerging. Foodomics is a new concept and practice to bring about "precision nutrition" and integrative bioengineering studies of food composition, quality, and safety, and applications to improve health of humans, animals, and other living organisms on the planet. Foodomics signals a three-way convergence among (1) food engineering; (2) omics systems science technologies such as proteomics, metabolomics, glycomics; and (3) medical/life sciences. This horizon scanning expert review aims to challenge the current practices in food sciences and bioengineering so as to adopt foodomics and systems thinking in foodstuff analysis, with a focus on possible applications of metabolomics. Among the omics biotechnologies, metabolomics is one of the prominent analytical platforms of interest to both food engineers and medical researchers engaged in nutritional sciences, precision medicine, and systems medicine diagnostics. Medical and omics system scientists, and bioengineering scholars can mutually learn from their respective professional expertise. Moving forward, establishment of "Foodomics Think Tanks" is one conceivable strategy to integrate medical and food sciences innovation at a systems scale. With its rich history in food sciences and tradition of interdisciplinary scholarship, the Silk Road countries offer notable potential for synthesis of diverse knowledge strands necessary to realize the prospects of foodomics from Asia and Middle East to Europe.

  7. Restoration of badlands through applying bio-engineering techniques in active gully systems: Evidence from the Ecuadorian Andes

    Science.gov (United States)

    Borja, P.; Vanacker, V.; Alvarado, D.; Govers, G.

    2012-04-01

    A better insight in the processes controlling sediment generation, transport and deposition in badlands is necessary to enhance restoration of degraded soils through eco-engineering techniques. In this study, we evaluate the effect of different bio-engineering measures on soil and slope stability. Five micro-catchments (of 0.2 to 5 ha) were selected within a 3 km2 area in the lower part of the Loreto catchment (Southern Ecuadorian Andes). The micro-catchments differ only by land cover and degree of implementation of soil and water conservation measures. Bio-engineering techniques were used to construct dikes made of fascines of wooden sticks and earth-filled tires in active gully beds, where they are most efficient to reduce water and sediment transport. The experimental design consists of three micro-catchments within highly degraded lands: (DI) micro-catchment with bio-engineering measures concentrated in the active gully beds, (DF) with reforestation of Eucalyptus trees, and (DT) reference situation without any conservation measures. Two micro-catchments were monitored in agricultural lands with (AI) and without (AT) bio-engineering measures in the active gully beds. All catchments were equipped with San Dimas flumes to measure water flow, and sediment traps to monitor sediment export. In the (active) gully beds, various parameters related to gully stability (soil water content, bed elevation, vegetation cover, sedimentation/erosion) were monitored at weekly intervals. First results show that bio-engineering techniques are efficient to stabilize active gully beds through a reduction of the rapid concentration of excess rainfall and the sediment production and transfer. Fascines made of wooden sticks are far more efficient than earth-filled tires. Sediment deposition behind dikes is strongly dependent on precedent rainfall events, and the slope and vegetation cover of the gully floor. The sediment deposited facilitates colonization of the gully floor by native

  8. Bioengineered nisin A derivatives with enhanced activity against both Gram positive and Gram negative pathogens.

    Directory of Open Access Journals (Sweden)

    Des Field

    Full Text Available Nisin is a bacteriocin widely utilized in more than 50 countries as a safe and natural antibacterial food preservative. It is the most extensively studied bacteriocin, having undergone decades of bioengineering with a view to improving function and physicochemical properties. The discovery of novel nisin variants with enhanced activity against clinical and foodborne pathogens has recently been described. We screened a randomized bank of nisin A producers and identified a variant with a serine to glycine change at position 29 (S29G, with enhanced efficacy against S. aureus SA113. Using a site-saturation mutagenesis approach we generated three more derivatives (S29A, S29D and S29E with enhanced activity against a range of Gram positive drug resistant clinical, veterinary and food pathogens. In addition, a number of the nisin S29 derivatives displayed superior antimicrobial activity to nisin A when assessed against a range of Gram negative food-associated pathogens, including E. coli, Salmonella enterica serovar Typhimurium and Cronobacter sakazakii. This is the first report of derivatives of nisin, or indeed any lantibiotic, with enhanced antimicrobial activity against both Gram positive and Gram negative bacteria.

  9. Comparison of bioengineered human bone construct from four sources of osteogenic cells.

    Science.gov (United States)

    Ng, Angela Min-Hwei; Saim, Aminuddin Bin; Tan, Kok-Keong; Tan, G H; Mokhtar, Sabarul Afian; Rose, Isa Mohamed; Othman, Fauziah; Idrus, Ruszymah Binti Haji

    2005-01-01

    Osteoprogenitor cells have been reported to be present in periosteum, cancellous and cortical bone, and bone marrow; but no attempt to identify the best cell source for bone tissue engineering has yet been reported. In this study, we aimed to investigate the growth and differentiation pattern of cells derived from these four sources in terms of cell doubling time and expression of osteoblast-specific markers in both monolayer cells and three-dimensional cell constructs in vitro. In parallel, human plasma derived-fibrin was evaluated for use as biomaterial when forming three-dimensional bone constructs. Our findings showed osteoprogenitor cells derived from periosteum to be most proliferative followed by cortical bone, cancellous bone, and then bone marrow aspirate. Bone-forming activity was observed in constructs formed with cells derived from periosteum, whereas calcium deposition was seen throughout the constructs formed with cells derived from cancellous and cortical bones. Although no mineralization activity was seen in constructs formed with osteoprogenitor cells derived from bone marrow, well-organized lacunae as would appear in the early phase of bone reconstruction were noted. Scanning electron microscopy evaluation showed cell proliferation throughout the fibrin matrix, suggesting the possible application of human fibrin as the bioengineered tissue scaffold at non-load-bearing sites.

  10. Dental Pulp Stem Cells as a multifaceted tool for bioengineering and the regeneration of craniomaxillofacial tissues

    Directory of Open Access Journals (Sweden)

    Maitane eAurrekoetxea

    2015-10-01

    Full Text Available Dental pulp stem cells, or DPSC, are neural crest-derived cells with an outstanding capacity to differentiate along multiple cell lineages of interest for cell therapy. In particular, highly efficient osteo/dentinogenic differentiation of DPSC can be achieved using simple in vitro protocols, making these cells a very attractive and promising tool for the future treatment of dental and periodontal diseases. Among craniomaxillofacial organs, the tooth and salivary gland are two such cases in which complete regeneration by tissue engineering using DPSC appears to be possible, as research over the last decade has made substantial progress in experimental models of partial or total regeneration of both organs, by cell recombination technology. Moreover, DPSC seem to be a particularly good choice for the regeneration of nerve tissues, including injured or transected cranial nerves. In this context, the oral cavity appears to be an excellent testing ground for new regenerative therapies using DPSC. However, many issues and challenges need yet to be addressed before these cells can be employed in clinical therapy. In this review, we point out some important aspects on the biology of DPSC with regard to their use for the reconstruction of different craniomaxillofacial tissues and organs, with special emphasis on cranial bones, nerves, teeth, and salivary glands. We suggest new ideas and strategies to fully exploit the capacities of DPSC for bioengineering of the aforementioned tissues.

  11. Torn ACL: A New Bioengineered Substitute Brought from the Laboratory to the Knee Joint

    Directory of Open Access Journals (Sweden)

    Francine Goulet

    2004-01-01

    Full Text Available Anterior cruciate ligament (ACL injuries occur at an annual rate of 120 000 in the USA, and many need reconstructive surgery. We report successful results at 1–13 months following implantation of bioengineered ACL (bACL in goats. A bACL has been developed using autologous ACL cells, a collagen matrix and bone plugs. The extremities of the bACL were fully integrated into the femur and tibia of the host. Vascularisation of the grafts was extensive 1 month post-surgery and improved with time. At 6 months post-grafting, histological and ultrastructural observations demonstrated a highly organised ligamentous structure, rich in type I collagen fibres and fibroblasts. At the implants' insertion sites, characteristic fibrocartilage was observed having well aligned chondrocytes and collagen fibrils. After a year, mechanical rupture of the grafts demonstrated a major gain in strength. Eventual applications of this new technology in humans include multiple uses in orthopaedic, dental and reconstructive surgeries.

  12. Flash NanoPrecipitation (FNP) for bioengineering nanoparticles to enhance the bioavailability

    Science.gov (United States)

    Feng, Jie; Zhang, Yingyue; McManus, Simone; Prud'Homme, Robert

    2017-11-01

    Nanoparticles for the delivery of therapeutics have been one of the successful areas in biomedical nanotechnology. Nanoparticles improve bioavailability by 1) the higher surface-to-volume ratios, enhancing dissolution rates, and 2) trapping drug molecules in higher energy, amorphous states for a higher solubility. However, conventional direct precipitation to prepare nanoparticles has the issues of low loading and encapsulation efficiency. Here we demonstrate a kinetically controlled and rapid-precipitation process called Flash NanoPrecipitation (FNP), to offer a multi-phase mixing platform for bioengineering nanoparticles. With the designed geometry in the micro-mixer, we can generate nanoparticles with a narrow size distribution, while maintaining high loading and encapsulation efficiency. By controlling the time scales in FNP, we can tune the nanoparticle size and the robustness of the process. Remarkably, the dissolution rates of the nanoparticles are significantly improved compared with crystalline drug powders. Furthermore, we investigate how to recover the drug-loaded nanoparticles from the aqueous dispersions. Regarding the maintenance of the bioavailability, we discuss the advantages and disadvantages of each drying process. These results suggest that FNP offers a versatile and scalable nano-fabrication platform for biomedical engineering.

  13. Bioengineering of rFVIIa Biopharmaceutical and Structure Characterization for Biosimilarity Assessment

    Directory of Open Access Journals (Sweden)

    Othman Montacir

    2018-01-01

    Full Text Available Eptacog alfa (NovoSeven® is a vitamin K-dependent recombinant Factor VIIa produced by genetic engineering from baby hamster kidney (BHK cells as a single peptide chain of 406 residues. After activation, it consists of a light chain (LC of 152 amino and a heavy chain (HC of 254 amino acids. Recombinant FVIIa undergoes many post-translational modifications (PTMs. The first ten glutamic acids of the N-terminal moiety are γ-carboxylated, Asn145 and Asn322 are N-glycosylated, and Ser52 and Ser60 are O-glycosylated. A head-to-head biosimilarity study was conducted for the originator and the first biosimilar AryoSeven™ to evaluate comparable bioengineering. Physicochemical properties were analyzed based on mass spectrometry, including intact mass, PTMs and higher-order structure. Both biotherapeutics exhibit a batch-to-batch variability in their N-glycan profiles. N-Glycopeptide analysis with UHPLC-QTOF-MSE confirmed N-glycosylation sites as well as two different O-glycopeptide sites. Ser60 was found to be O-fucosylated and Ser52 had O-glucose or O-glucose-(xylose1,2 motifs as glycan variants. Ion mobility spectrometry (TWIMS and NMR spectroscopy data affirm close similarity of the higher-order structure of both biologicals. Potency of the biodrugs was analyzed by a coagulation assay demonstrating comparable bioactivity. Consequently, careful process optimization led to a stable production process of the biopharmaceuticals.

  14. Teaching statics of fluids in bioengineering: a multidisciplinary proposal based on competences

    Science.gov (United States)

    Alborch, A.; Puzzella, A.; Lopez, N.; Cabrera, L.; Zabala, A.; Demartini, H.

    2007-11-01

    The aim of this work is to share the findings of an educational experience undertaken by first-year university students of bioengineering, oriented towards the model of Competence-based Education. Different aspects on integrative education pursued in the subject goals are explicitly focused here by designing a strategy within a contextualized and multidisciplinary approach that combines knowledge from Physics, Chemistry and Biology. The topic chosen for the work is Static of Fluids, because it allows relating pressure to its biological effects on human beings. After evaluating a pre-test, new interrelated strategies are implemented. Due to the motivation audiovisuals generate in adolescents, we start showing an argumentative film entitled 'The Big Blue', and continue with different individual and/or group activities, finishing with a post-test to assess the development of the competences proposed. Results are encouraging as regards the level of specific competences acquired and, complementarily, basic and professional competences in general. Besides, the experience met expectations as regards student motivation, interest and commitment to learning, which ensured the path taken by the academicians by means of implementing innovative strategies.

  15. Microalgal bioengineering for sustainable energy development: Recent transgenesis and metabolic engineering strategies.

    Science.gov (United States)

    Banerjee, Chiranjib; Singh, Puneet Kumar; Shukla, Pratyoosh

    2016-03-01

    Exploring the efficiency of algae to produce remarkable products can be directly benefitted by studying its mechanism at systems level. Recent advents in biotechnology like flux balance analysis (FBA), genomics and in silico proteomics minimize the wet lab exertion. It is understood that FBA predicts the metabolic products, metabolic pathways and alternative pathway to maximize the desired product, and these are key components for microalgae bio-engineering. This review encompasses recent transgenesis techniques and metabolic engineering strategies applied to different microalgae for improving different traits. Further it also throws light on RNAi and riboswitch engineering based methods which may be advantageous for high throughput microalgal research. A valid and optimally designed microalga can be developed where every engineering strategies meet each other successfully and will definitely fulfill the market needs. It is also to be noted that Omics (viz. genetic and metabolic manipulation with bioinformatics) should be integrated to develop a strain which could prove to be a futuristic solution for sustainable development for energy. Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Scorpion Toxins Specific for Potassium (K+ Channels: A Historical Overview of Peptide Bioengineering

    Directory of Open Access Journals (Sweden)

    Zachary L. Bergeron

    2012-11-01

    Full Text Available Scorpion toxins have been central to the investigation and understanding of the physiological role of potassium (K+ channels and their expansive function in membrane biophysics. As highly specific probes, toxins have revealed a great deal about channel structure and the correlation between mutations, altered regulation and a number of human pathologies. Radio- and fluorescently-labeled toxin isoforms have contributed to localization studies of channel subtypes in expressing cells, and have been further used in competitive displacement assays for the identification of additional novel ligands for use in research and medicine. Chimeric toxins have been designed from multiple peptide scaffolds to probe channel isoform specificity, while advanced epitope chimerization has aided in the development of novel molecular therapeutics. Peptide backbone cyclization has been utilized to enhance therapeutic efficiency by augmenting serum stability and toxin half-life in vivo as a number of K+-channel isoforms have been identified with essential roles in disease states ranging from HIV, T-cell mediated autoimmune disease and hypertension to various cardiac arrhythmias and Malaria. Bioengineered scorpion toxins have been monumental to the evolution of channel science, and are now serving as templates for the development of invaluable experimental molecular therapeutics.

  17. Bioengineered Nisin A Derivatives with Enhanced Activity against Both Gram Positive and Gram Negative Pathogens

    Science.gov (United States)

    Field, Des; Begley, Maire; O’Connor, Paula M.; Daly, Karen M.; Hugenholtz, Floor; Cotter, Paul D.; Hill, Colin; Ross, R. Paul

    2012-01-01

    Nisin is a bacteriocin widely utilized in more than 50 countries as a safe and natural antibacterial food preservative. It is the most extensively studied bacteriocin, having undergone decades of bioengineering with a view to improving function and physicochemical properties. The discovery of novel nisin variants with enhanced activity against clinical and foodborne pathogens has recently been described. We screened a randomized bank of nisin A producers and identified a variant with a serine to glycine change at position 29 (S29G), with enhanced efficacy against S. aureus SA113. Using a site-saturation mutagenesis approach we generated three more derivatives (S29A, S29D and S29E) with enhanced activity against a range of Gram positive drug resistant clinical, veterinary and food pathogens. In addition, a number of the nisin S29 derivatives displayed superior antimicrobial activity to nisin A when assessed against a range of Gram negative food-associated pathogens, including E. coli, Salmonella enterica serovar Typhimurium and Cronobacter sakazakii. This is the first report of derivatives of nisin, or indeed any lantibiotic, with enhanced antimicrobial activity against both Gram positive and Gram negative bacteria. PMID:23056510

  18. Teaching statics of fluids in bioengineering: a multidisciplinary proposal based on competences

    International Nuclear Information System (INIS)

    Alborch, A; Puzzella, A; Lopez, N; Cabrera, L; Zabala, A; Demartini, H

    2007-01-01

    The aim of this work is to share the findings of an educational experience undertaken by first-year university students of bioengineering, oriented towards the model of Competence-based Education. Different aspects on integrative education pursued in the subject goals are explicitly focused here by designing a strategy within a contextualized and multidisciplinary approach that combines knowledge from Physics, Chemistry and Biology. The topic chosen for the work is Static of Fluids, because it allows relating pressure to its biological effects on human beings. After evaluating a pre-test, new interrelated strategies are implemented. Due to the motivation audiovisuals generate in adolescents, we start showing an argumentative film entitled 'The Big Blue', and continue with different individual and/or group activities, finishing with a post-test to assess the development of the competences proposed. Results are encouraging as regards the level of specific competences acquired and, complementarily, basic and professional competences in general. Besides, the experience met expectations as regards student motivation, interest and commitment to learning, which ensured the path taken by the academicians by means of implementing innovative strategies

  19. Single-cell analyses identify bioengineered niches for enhanced maintenance of hematopoietic stem cells.

    Science.gov (United States)

    Roch, Aline; Giger, Sonja; Girotra, Mukul; Campos, Vasco; Vannini, Nicola; Naveiras, Olaia; Gobaa, Samy; Lutolf, Matthias P

    2017-08-09

    The in vitro expansion of long-term hematopoietic stem cells (HSCs) remains a substantial challenge, largely because of our limited understanding of the mechanisms that control HSC fate choices. Using single-cell multigene expression analysis and time-lapse microscopy, here we define gene expression signatures and cell cycle hallmarks of murine HSCs and the earliest multipotent progenitors (MPPs), and analyze systematically single HSC fate choices in culture. Our analysis revealed twelve differentially expressed genes marking the quiescent HSC state, including four genes encoding cell-cell interaction signals in the niche. Under basal culture conditions, most HSCs rapidly commit to become early MPPs. In contrast, when we present ligands of the identified niche components such as JamC or Esam within artificial niches, HSC cycling is reduced and long-term multipotency in vivo is maintained. Our approach to bioengineer artificial niches should be useful in other stem cell systems.Haematopoietic stem cell (HSC) self-renewal is not sufficiently understood to recapitulate in vitro. Here, the authors generate gene signature and cell cycle hallmarks of single murine HSCs, and use identified endothelial receptors Esam and JamC as substrates to enhance HSC growth in engineered niches.

  20. Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis.

    Science.gov (United States)

    Jiang, Nan; Zhou, Jian; Chen, Mo; Schiff, Michael D; Lee, Chang H; Kong, Kimi; Embree, Mildred C; Zhou, Yanheng; Mao, Jeremy J

    2014-02-01

    Rodent incisors provide a classic model for studying epithelial-mesenchymal interactions in development. However, postnatal stem/progenitor cells in rodent incisors have not been exploited for tooth regeneration. Here, we characterized postnatal rat incisor epithelium and mesenchyme stem/progenitor cells and found that they formed enamel- and dentin-like tissues in vivo. Epithelium and mesenchyme cells were harvested separately from the apical region of postnatal 4-5 day rat incisors. Epithelial and mesenchymal phenotypes were confirmed by immunocytochemistry, CFU assay and/or multi-lineage differentiation. CK14+, Sox2+ and Lgr5+ epithelium stem cells from the cervical loop enhanced amelogenin and ameloblastin expression upon BMP4 or FGF3 stimulation, signifying their differentiation towards ameloblast-like cells, whereas mesenchyme stem/progenitor cells upon BMP4, BMP7 and Wnt3a treatment robustly expressed Dspp, a hallmark of odontoblastic differentiation. We then control-released microencapsulated BMP4, BMP7 and Wnt3a in transplants of epithelium and mesenchyme stem/progenitor cells in the renal capsule of athymic mice in vivo. Enamel and dentin-like tissues were generated in two integrated layers with specific expression of amelogenin and ameloblastin in the newly formed, de novo enamel-like tissue, and DSP in dentin-like tissue. These findings suggest that postnatal epithelium and mesenchyme stem/progenitor cells can be primed towards bioengineered tooth regeneration. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. 8th Argentinean Bioengineering Society Conference (SABI 2011) and 7th Clinical Engineering Meeting

    Science.gov (United States)

    Meschino, Gustavo Javier; Ballarin, Virginia L.

    2011-12-01

    In September 2011, the Eighteenth Edition of the Argentinean Bioengineering Society Conference (SABI 2011) and Seventh Clinical Engineering Meeting were held in Mar del Plata, Argetina. The Mar del Plata SABI Regional and the School of Engineering of the Universidad Nacional de Mar del Plata invited All bioengineers, engineers, physicists, mathematicians, biologists, physicians and health professionals working in the field of Bioengineering to participate in this event. The overall objectives of the Conference were: To provide discussion of scientific research results in Bioengineering and Clinical Engineering. To promote technological development experiences. To strengthen the institutional and scientific communication links in the area of Bioengineering, mainly between Universities of Latin America. To encourage students, teachers, researchers and professionals to establish exchanges of experiences and knowledge. To provide biomedical engineering technology solutions to the society and contributing ideas for low cost care. Conference photograph Conference photograph Conference photograph Conference photograph EXECUTIVE COMMITTEE SABI 2011 Chair Dra Virginia L Ballarin Universidad Nacional de Mar del Plata Co-Chair Dra Teresita R Cuadrado Universidad Nacional de Mar del Plata - CONICET Local Comittee Dr Gustavo Abraham Universidad Nacional de Mar del Plata - CONICET Dra Josefina Ballarre Universidad Nacional de Mar del Plata - CONICET Dr Eduardo Blotta Universidad Nacional de Mar del Plata Dra Agustina Bouchet Universidad Nacional de Mar del Plata Dr Marcel Brun Universidad Nacional de Mar del Plata Dra Silvia Ceré Universidad Nacional de Mar del Plata - CONICET Dra Mariela Azul Gonzalez Universidad Nacional de Mar del Plata - CONICET Dra Lucia Isabel Passoni Universidad Nacional de Mar del Plata Dr Juan Ignacio Pastore Universidad Nacional de Mar del Plata - CONICET Dra Adriana Scandurra Universidad Nacional de Mar del Plata SCIENTIFIC ADVISORY COMMITTEE

  2. Water-Quality Changes Caused by Riverbank Filtration Between the Missouri River and Three Pumping Wells of the Independence, Missouri, Well Field 2003-05

    Science.gov (United States)

    Kelly, Brian P.; Rydlund, Jr., Paul H.

    2006-01-01

    Riverbank filtration substantially improves the source-water quality of the Independence, Missouri well field. Coliform bacteria, Cryptosporidium, Giardia, viruses and selected constituents were analyzed in water samples from the Missouri River, two vertical wells, and a collector well. Total coliform bacteria, Cryptosporidium, Giardia, and total culturable viruses were detected in the Missouri River, but were undetected in samples from wells. Using minimum reporting levels for non-detections in well samples, minimum log removals were 4.57 for total coliform bacteria, 1.67 for Cryptosporidium, 1.67 for Giardia, and 1.15 for total culturable virus. Ground-water flow rates between the Missouri River and wells were calculated from water temperature profiles and ranged between 1.2 and 6.7 feet per day. Log removals based on sample pairs separated by the traveltime between the Missouri River and wells were infinite for total coliform bacteria (minimum detection level equal to zero), between 0.8 and 3.5 for turbidity, between 1.5 and 2.1 for Giardia, and between 0.4 and 2.6 for total culturable viruses. Cryptosporidium was detected once in the Missouri River but no corresponding well samples were available. No clear relation was evident between changes in water quality in the Missouri River and in wells for almost all constituents. Results of analyses for organic wastewater compounds and the distribution of dissolved oxygen, specific conductance, and temperature in the Missouri River indicate water quality on the south side of the river was moderately influenced by the south bank inflows to the river upstream from the Independence well field.

  3. Surface modification of electrospun PLGA scaffold with collagen for bioengineered skin substitutes

    International Nuclear Information System (INIS)

    Sadeghi, A.R.; Nokhasteh, S.; Molavi, A.M.; Khorsand-Ghayeni, M.; Naderi-Meshkin, H.; Mahdizadeh, A.

    2016-01-01

    In skin tissue engineering, surface feature of the scaffolds plays an important role in cell adhesion and proliferation. In this study, non-woven fibrous substrate based on poly (lactic-co-glycolic acid) (PLGA) (75/25) were hydrolyzed in various concentrations of NaOH (0.05 N, 0.1 N, 0.3 N) to increase carboxyl and hydroxyl groups on the fiber surfaces. These functional groups were activated by EDC/NHS to create chemical bonding with collagen. To improve bioactivity, the activated substrates were coated with a collagen solution (2 mg/ml) and cross-linking was carried out using the EDC/NHS in MES buffer. The effectiveness of the method was evaluated by contact angle measurements, porosimetry, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), tensile and degradation tests as well as in vitro cell attachment and cytotoxicity assays. Cell culture results of human dermal fibroblasts (HDF) and keratinocytes cell line (HaCat) revealed that the cells could attach to the scaffold. Further investigation with MTT assay showed that the cell proliferation of HaCat significantly increases with collagen coating. It seems that sufficient stability of collagen on the surface due to proper chemical bonding and cross-linking has increased the bioactivity of surface remarkably which can be promising for bioengineered skin applications. - Highlights: • Surface activation was carried out by hydrolysis of PLGA fibers. • To improve bioactivity, the activated samples were coated with a collagen solution. • Functional groups were activated by EDC/NHS to create chemical bonding with collagen. • Cross-linking of collagen was carried out using EDC/NHS in MES buffer. • The coated samples exhibited better adhesion and proliferation of epidermal cells.

  4. Extraction and characterization of highly purified collagen from bovine pericardium for potential bioengineering applications

    International Nuclear Information System (INIS)

    Santos, Maria Helena; Silva, Rafael M.; Dumont, Vitor C.; Neves, Juliana S.; Mansur, Herman S.; Heneine, Luiz Guilherme D.

    2013-01-01

    Bovine pericardium is widely used as a raw material in bioengineering as a source of collagen, a fundamental structural molecule. The physical, chemical, and biocompatibility characteristics of these natural fibers enable their broad use in several areas of the health sciences. For these applications, it is important to obtain collagen of the highest possible purity. The lack of a method to produce these pure biocompatible materials using simple and economically feasible techniques presents a major challenge to their production on an industrial scale. This study aimed to extract, purify, and characterize the type I collagen protein originating from bovine pericardium, considered to be an abundant tissue resource. The pericardium tissue was collected from male animals at slaughter age. Pieces of bovine pericardium were enzymatically digested, followed by a novel protocol developed for protein purification using ion-exchange chromatography. The material was extensively characterized by electrophoresis, scanning electron microscopy, energy dispersive X-ray spectroscopy, and infrared spectroscopy. The results showed a purified material with morphological properties and chemical functionalities compatible with type I collagen and similar to a highly purified commercial collagen. Thus, an innovative and relatively simple processing method was developed to extract and purify type I collagen from bovine tissue with potential applications as a biomaterial for regenerative tissue engineering. - Highlights: ► Type I collagen was obtained from bovine pericardium, an abundant tissue resource. ► A simple and feasible processing technique was developed to purify bovine collagen. ► The appropriate process may be performed on industrial scale. ► The pure collagen presented appropriate morphological and molecular characteristics. ► The purify collagen has shown potential use as a biomaterial in tissue engineering.

  5. Surface modification of electrospun PLGA scaffold with collagen for bioengineered skin substitutes

    Energy Technology Data Exchange (ETDEWEB)

    Sadeghi, A.R., E-mail: sadeghi_av@ymail.com [Materials Research Group, Iranian Academic Center for Education, Culture and Research, (ACECR), Mashhad Branch, Mashhad (Iran, Islamic Republic of); Nokhasteh, S. [Materials Research Group, Iranian Academic Center for Education, Culture and Research, (ACECR), Mashhad Branch, Mashhad (Iran, Islamic Republic of); Molavi, A.M. [Materials Research Group, Iranian Academic Center for Education, Culture and Research, (ACECR), Mashhad Branch, Mashhad (Iran, Islamic Republic of); Materials Engineering Department, Tarbiat Modares University, Tehran (Iran, Islamic Republic of); Khorsand-Ghayeni, M. [Materials Research Group, Iranian Academic Center for Education, Culture and Research, (ACECR), Mashhad Branch, Mashhad (Iran, Islamic Republic of); Naderi-Meshkin, H. [Stem Cell and Regenerative Medicine Research Department, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad (Iran, Islamic Republic of); Mahdizadeh, A. [Nanotechnology Institute, University of Sistan and Baluchestan, Zahedan (Iran, Islamic Republic of)

    2016-09-01

    In skin tissue engineering, surface feature of the scaffolds plays an important role in cell adhesion and proliferation. In this study, non-woven fibrous substrate based on poly (lactic-co-glycolic acid) (PLGA) (75/25) were hydrolyzed in various concentrations of NaOH (0.05 N, 0.1 N, 0.3 N) to increase carboxyl and hydroxyl groups on the fiber surfaces. These functional groups were activated by EDC/NHS to create chemical bonding with collagen. To improve bioactivity, the activated substrates were coated with a collagen solution (2 mg/ml) and cross-linking was carried out using the EDC/NHS in MES buffer. The effectiveness of the method was evaluated by contact angle measurements, porosimetry, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), tensile and degradation tests as well as in vitro cell attachment and cytotoxicity assays. Cell culture results of human dermal fibroblasts (HDF) and keratinocytes cell line (HaCat) revealed that the cells could attach to the scaffold. Further investigation with MTT assay showed that the cell proliferation of HaCat significantly increases with collagen coating. It seems that sufficient stability of collagen on the surface due to proper chemical bonding and cross-linking has increased the bioactivity of surface remarkably which can be promising for bioengineered skin applications. - Highlights: • Surface activation was carried out by hydrolysis of PLGA fibers. • To improve bioactivity, the activated samples were coated with a collagen solution. • Functional groups were activated by EDC/NHS to create chemical bonding with collagen. • Cross-linking of collagen was carried out using EDC/NHS in MES buffer. • The coated samples exhibited better adhesion and proliferation of epidermal cells.

  6. Bioengineered human IAS reconstructs with functional and molecular properties similar to intact IAS

    Science.gov (United States)

    Singh, Jagmohan

    2012-01-01

    Because of its critical importance in rectoanal incontinence, we determined the feasibility to reconstruct internal anal sphincter (IAS) from human IAS smooth muscle cells (SMCs) with functional and molecular attributes similar to the intact sphincter. The reconstructs were developed using SMCs from the circular smooth muscle layer of the human IAS, grown in smooth muscle differentiation media under sterile conditions in Sylgard-coated tissue culture plates with central Sylgard posts. The basal tone in the reconstructs and its changes were recorded following 0 Ca2+, KCl, bethanechol, isoproterenol, protein kinase C (PKC) activator phorbol 12,13-dibutyrate, and Rho kinase (ROCK) and PKC inhibitors Y-27632 and Gö-6850, respectively. Western blot (WB), immunofluorescence (IF), and immunocytochemical (IC) analyses were also performed. The reconstructs developed spontaneous tone (0.68 ± 0.26 mN). Bethanechol (a muscarinic agonist) and K+ depolarization produced contraction, whereas isoproterenol (β-adrenoceptor agonist) and Y-27632 produced a concentration-dependent decrease in the tone. Maximal decrease in basal tone with Y-27632 and Gö-6850 (each 10−5 M) was 80.45 ± 3.29 and 17.76 ± 3.50%, respectively. WB data with the IAS constructs′ SMCs revealed higher levels of RhoA/ROCK, protein kinase C-potentiated inhibitor or inhibitory phosphoprotein for myosin phosphatase (CPI-17), phospho-CPI-17, MYPT1, and 20-kDa myosin light chain vs. rectal smooth muscle. WB, IF, and IC studies of original SMCs and redispersed from the reconstructs for the relative distribution of different signal transduction proteins confirmed the feasibility of reconstruction of IAS with functional properties similar to intact IAS and demonstrated the development of myogenic tone with critical dependence on RhoA/ROCK. We conclude that it is feasible to bioengineer IAS constructs using human IAS SMCs that behave like intact IAS. PMID:22790596

  7. Living part on soil bioengineering structures in Appennino Tosco-emiliano

    Science.gov (United States)

    Guastini, Enrico; Preti, Federico; Dani, Andrea

    2014-05-01

    From analisys headed up in soil bioengineered areas in different parts of Tuscany, the suitest root systems in slope stabilization turn out to be those spreading from seed-born plants, while adventitious roots from cuttings are often absent in the part more distant from the neck, and in some cases are insufficient to grant life support just in case of minor stress conditions. Genus Alnus shows it's adaptation capability to restore initial restoration steps and to create renovation prerequisites for other species through ammending litter production and symbiosis for nitrogen fixation with Frankia genus bacteria; other similar symbiosis (with Rhizobium and fungi) are carried out by Robinia pseudacacia. Soil fecundity increase is confirmed by the following entrance of more demanding species, as Ostrya carpinifolia and Acer pseudoplatanus at the tree level, Urtica dioica and Rubus Ulmifolius (nitrophilouses) at grass level. In the project phase it ought to imagine a well-structured implant, including rooted plants, cuttings and posibly a seed mix of colonising species aiming to form a germplasm on the structure itself in order to sprout whenever the local conditions allow it. Verifying that many after developed species came from ornithocore dissemination (Ficus carica, Pinus spp., Rosa canina, Sambucus nigra), lead to toughts about bedding out bird-attracting species on structures in order to realise a faster (and maybe more complex) succession development. This higher velocity could grant in a shorter period the production of a root mass spread in a more disomogeneous and complex pattern than that deriving from cuttings disposed in the traditional way; such a variability could allow a better interaction with other biological factors in the soil (bacteria, fungi, nematodes, ...) that are important for the plant nutrient cicle (Ohsowski et al., 2012) and then the constituion of an articulate, long-term system.

  8. Molecular and bioengineering strategies to improve alginate and polydydroxyalkanoate production by Azotobacter vinelandii

    Directory of Open Access Journals (Sweden)

    Espín Guadalupe

    2007-02-01

    Full Text Available Abstract Several aspects of alginate and PHB synthesis in Azotobacter vinelandii at a molecular level have been elucidated in articles published during the last ten years. It is now clear that alginate and PHB synthesis are under a very complex genetic control. Genetic modification of A. vinelandii has produced a number of very interesting mutants which have particular traits for alginate production. One of these mutants has been shown to produce the alginate with the highest mean molecular mass so far reported. Recent work has also shed light on the factors determining molecular mass distribution; the most important of these being identified as; dissolved oxygen tension and specific growth rate. The use of specific mutants has been very useful for the correct analysis and interpretation of the factors affecting polymerization. Recent scale-up/down work on alginate production has shown that oxygen limitation is crucial for producing alginate of high molecular mass, a condition which is optimized in shake flasks and which can now be reproduced in stirred fermenters. It is clear that the phenotypes of mutants grown on plates are not necessarily reproducible when the strains are tested in lab or bench scale fermenters. In the case of PHB, A. vinelandii has shown itself able to produce relatively large amounts of this polymer of high molecular weight on cheap substrates, even allowing for simple extraction processes. The development of fermentation strategies has also shown promising results in terms of improving productivity. The understanding of the regulatory mechanisms involved in the control of PHB synthesis, and of its metabolic relationships, has increased considerably, making way for new potential strategies for the further improvement of PHB production. Overall, the use of a multidisciplinary approach, integrating molecular and bioengineering aspects is a necessity for optimizing alginate and PHB production in A. vinelandii.

  9. Phenotypic characterization of prostate cancer LNCaP cells cultured within a bioengineered microenvironment.

    Directory of Open Access Journals (Sweden)

    Shirly Sieh

    Full Text Available Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D cultures lack the essential characteristics of the native cellular microenvironment, three-dimensional (3D cultures have been developed to better mimic the natural extracellular matrix. To date, 3D culture systems have relied mostly on collagen and Matrigel™ hydrogels, allowing only limited control over matrix stiffness, proteolytic degradability, and ligand density. In contrast, bioengineered hydrogels allow us to independently tune and systematically investigate the influence of these parameters on cell growth and differentiation. In this study, polyethylene glycol (PEG hydrogels, functionalized with the Arginine-glycine-aspartic acid (RGD motifs, common cell-binding motifs in extracellular matrix proteins, and matrix metalloproteinase (MMP cleavage sites, were characterized regarding their stiffness, diffusive properties, and ability to support growth of androgen-dependent LNCaP prostate cancer cells. We found that the mechanical properties modulated the growth kinetics of LNCaP cells in the PEG hydrogel. At culture periods of 28 days, LNCaP cells underwent morphogenic changes, forming tumor-like structures in 3D culture, with hypoxic and apoptotic cores. We further compared protein and gene expression levels between 3D and 2D cultures upon stimulation with the synthetic androgen R1881. Interestingly, the kinetics of R1881 stimulated androgen receptor (AR nuclear translocation differed between 2D and 3D cultures when observed by immunofluorescent staining. Furthermore, microarray studies revealed that changes in expression levels of androgen responsive genes upon R1881 treatment differed greatly between 2D and 3D cultures. Taken together, culturing LNCaP cells in the tunable PEG hydrogels reveals differences in the cellular responses to

  10. Extraction and characterization of highly purified collagen from bovine pericardium for potential bioengineering applications

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Maria Helena, E-mail: mariahelena.santos@gmail.com [Department of Dentistry, Federal University of Vales do Jequitinhonha e Mucuri-UFVJM, Diamantina/MG 39100-000 (Brazil); Center for Assessment and Development of Biomaterials-BioMat, Federal University of Vales do Jequitinhonha e Mucuri-UFVJM, Diamantina/MG 39100-000 (Brazil); Silva, Rafael M.; Dumont, Vitor C. [Department of Dentistry, Federal University of Vales do Jequitinhonha e Mucuri-UFVJM, Diamantina/MG 39100-000 (Brazil); Center for Assessment and Development of Biomaterials-BioMat, Federal University of Vales do Jequitinhonha e Mucuri-UFVJM, Diamantina/MG 39100-000 (Brazil); Neves, Juliana S. [Center for Assessment and Development of Biomaterials-BioMat, Federal University of Vales do Jequitinhonha e Mucuri-UFVJM, Diamantina/MG 39100-000 (Brazil); Mansur, Herman S. [Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais-UFMG, Belo Horizonte/MG 31270-901 (Brazil); Heneine, Luiz Guilherme D. [Department of Health Science, Ezequiel Dias Foundation-FUNED, Belo Horizonte/MG 30510-010 (Brazil)

    2013-03-01

    Bovine pericardium is widely used as a raw material in bioengineering as a source of collagen, a fundamental structural molecule. The physical, chemical, and biocompatibility characteristics of these natural fibers enable their broad use in several areas of the health sciences. For these applications, it is important to obtain collagen of the highest possible purity. The lack of a method to produce these pure biocompatible materials using simple and economically feasible techniques presents a major challenge to their production on an industrial scale. This study aimed to extract, purify, and characterize the type I collagen protein originating from bovine pericardium, considered to be an abundant tissue resource. The pericardium tissue was collected from male animals at slaughter age. Pieces of bovine pericardium were enzymatically digested, followed by a novel protocol developed for protein purification using ion-exchange chromatography. The material was extensively characterized by electrophoresis, scanning electron microscopy, energy dispersive X-ray spectroscopy, and infrared spectroscopy. The results showed a purified material with morphological properties and chemical functionalities compatible with type I collagen and similar to a highly purified commercial collagen. Thus, an innovative and relatively simple processing method was developed to extract and purify type I collagen from bovine tissue with potential applications as a biomaterial for regenerative tissue engineering. - Highlights: Black-Right-Pointing-Pointer Type I collagen was obtained from bovine pericardium, an abundant tissue resource. Black-Right-Pointing-Pointer A simple and feasible processing technique was developed to purify bovine collagen. Black-Right-Pointing-Pointer The appropriate process may be performed on industrial scale. Black-Right-Pointing-Pointer The pure collagen presented appropriate morphological and molecular characteristics. Black-Right-Pointing-Pointer The purify

  11. Plasticity of human dental pulp stromal cells with bioengineering platforms: a versatile tool for regenerative medicine.

    Science.gov (United States)

    Barachini, Serena; Danti, Serena; Pacini, Simone; D'Alessandro, Delfo; Carnicelli, Vittoria; Trombi, Luisa; Moscato, Stefania; Mannari, Claudio; Cei, Silvia; Petrini, Mario

    2014-12-01

    In recent years, human dental pulp stromal cells (DPSCs) have received growing attention due to their characteristics in common with other mesenchymal stem cells, in addition to the ease with which they can be harvested. In this study, we demonstrated that the isolation of DPSCs from third molar teeth of healthy individuals allowed the recovery of dental mesenchymal stem cells that showed self-renewal and multipotent differentiation capability. DPSCs resulted positive for CD73, CD90, CD105, STRO-1, negative for CD34, CD45, CD14 and were able to differentiate into osteogenic and chondrogenic cells. We also assayed the angiogenic potential of DPSCs, their capillary tube-like formation was assessed using an in vitro angiogenesis assay and the uptake of acetylated low-density lipoprotein was measured as a marker of endothelial function. Based on these results, DPSCs were capable of differentiating into cells with phenotypic and functional features of endothelial cells. Furthermore, this study investigated the growth and differentiation of human DPSCs under a variety of bioengineering platforms, such as low frequency ultrasounds, tissue engineering and nanomaterials. DPSCs showed an enhanced chondrogenic differentiation under ultrasound application. Moreover, DPSCs were tested on different scaffolds, poly(vinyl alcohol)/gelatin (PVA/G) sponges and human plasma clots. We showed that both PVA/G and human plasma clot are suitable scaffolds for adhesion, growth and differentiation of DPSCs toward osteoblastic lineages. Finally, we evaluated the interactions of DPSCs with a novel class of nanomaterials, namely boron nitride nanotubes (BNNTs). From our investigation, DPSCs have appeared as a highly versatile cellular tool to be employed in regenerative medicine. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays

    Science.gov (United States)

    Lam, Patricia; Gulati, Neetu M.; Stewart, Phoebe L.; Keri, Ruth A.; Steinmetz, Nicole F.

    2016-03-01

    The 2014 Ebola epidemic is the largest to date. There is no cure or treatment for this deadly disease; therefore there is an urgent need to develop new diagnostics to accurately detect Ebola. Current RT-PCR assays lack sensitive and reliable positive controls. To address this critical need, we devised a bio-inspired positive control for use in RT-PCR diagnostics: we encapsulated scrambled Ebola RNA sequences inside of tobacco mosaic virus to create a biomimicry that is non-infectious, but stable, and could therefore serve as a positive control in Ebola diagnostic assays. Here, we report the bioengineering and validation of this probe.

  13. Bioengineering a human plasma-based epidermal substitute with efficient grafting capacity and high content in clonogenic cells.

    Science.gov (United States)

    Alexaline, Maia M; Trouillas, Marina; Nivet, Muriel; Bourreau, Emilie; Leclerc, Thomas; Duhamel, Patrick; Martin, Michele T; Doucet, Christelle; Fortunel, Nicolas O; Lataillade, Jean-Jacques

    2015-06-01

    Cultured epithelial autografts (CEAs) produced from a small, healthy skin biopsy represent a lifesaving surgical technique in cases of full-thickness skin burn covering >50% of total body surface area. CEAs also present numerous drawbacks, among them the use of animal proteins and cells, the high fragility of keratinocyte sheets, and the immaturity of the dermal-epidermal junction, leading to heavy cosmetic and functional sequelae. To overcome these weaknesses, we developed a human plasma-based epidermal substitute (hPBES) for epidermal coverage in cases of massive burn, as an alternative to traditional CEA, and set up critical quality controls for preclinical and clinical studies. In this study, phenotypical analyses in conjunction with functional assays (clonal analysis, long-term culture, or in vivo graft) showed that our new substitute fulfills the biological requirements for epidermal regeneration. hPBES keratinocytes showed high potential for cell proliferation and subsequent differentiation similar to healthy skin compared with a well-known reference material, as ascertained by a combination of quality controls. This work highlights the importance of integrating relevant multiparameter quality controls into the bioengineering of new skin substitutes before they reach clinical development. This work involves the development of a new bioengineered epidermal substitute with pertinent functional quality controls. The novelty of this work is based on this quality approach. ©AlphaMed Press.

  14. Bioengineering of the Plant Culture of Capsicum frutescens with Vanillin Synthase Gene for the Production of Vanillin.

    Science.gov (United States)

    Chee, Marcus Jenn Yang; Lycett, Grantley W; Khoo, Teng-Jin; Chin, Chiew Foan

    2017-01-01

    Production of vanillin by bioengineering has gained popularity due to consumer demand toward vanillin produced by biological systems. Natural vanillin from vanilla beans is very expensive to produce compared to its synthetic counterpart. Current bioengineering works mainly involve microbial biotechnology. Therefore, alternative means to the current approaches are constantly being explored. This work describes the use of vanillin synthase (VpVAN), to bioconvert ferulic acid to vanillin in a plant system. The VpVAN enzyme had been shown to directly convert ferulic acid and its glucoside into vanillin and its glucoside, respectively. As the ferulic acid precursor and vanillin were found to be the intermediates in the phenylpropanoid biosynthetic pathway of Capsicum species, this work serves as a proof-of-concept for vanillin production using Capsicum frutescens (C. frutescens or hot chili pepper). The cells of C. frutescens were genetically transformed with a codon optimized VpVAN gene via biolistics. Transformed explants were selected and regenerated into callus. Successful integration of the gene cassette into the plant genome was confirmed by polymerase chain reaction. High-performance liquid chromatography was used to quantify the phenolic compounds detected in the callus tissues. The vanillin content of transformed calli was 0.057% compared to 0.0003% in untransformed calli.

  15. Environmental Toxin Screening Using Human-Derived 3D Bioengineered Liver and Cardiac Organoids

    Directory of Open Access Journals (Sweden)

    Steven D. Forsythe

    2018-04-01

    Full Text Available IntroductionEnvironmental toxins, such as lead and other heavy metals, pesticides, and other compounds, represent a significant health concern within the USA and around the world. Even in the twenty-first century, a plethora of cities and towns in the U.S. have suffered from exposures to lead in drinking water or other heavy metals in food or the earth, while there is a high possibility of further places to suffer such exposures in the near future.MethodsWe employed bioengineered 3D human liver and cardiac organoids to screen a panel of environmental toxins (lead, mercury, thallium, and glyphosate, and charted the response of the organoids to these compounds. Liver and cardiac organoids were exposed to lead (10 µM–10 mM, mercury (200 nM–200 µM, thallium (10 nM–10 µM, or glyphosate (25 µM–25 mM for a duration of 48 h. The impacts of toxin exposure were then assessed by LIVE/DEAD viability and cytotoxicity staining, measuring ATP activity and determining IC50 values, and determining changes in cardiac organoid beating activity.ResultsAs expected, all of the toxins induced toxicity in the organoids. Both ATP and LIVE/DEAD assays showed toxicity in both liver and cardiac organoids. In particular, thallium was the most toxic, with IC50 values of 13.5 and 1.35 µM in liver and cardiac organoids, respectively. Conversely, glyphosate was the least toxic of the four compounds, with IC50 values of 10.53 and 10.85 mM in liver and cardiac organoids, respectively. Additionally, toxins had a negative influence on cardiac organoid beating activity as well. Thallium resulting in the most significant decreases in beating rate, followed by mercury, then glyphosate, and finally, lead. These results suggest that the 3D organoids have significant utility to be deployed in additional toxicity screening applications, and future development of treatments to mitigate exposures.Conclusion3D organoids have significant utility to be

  16. Capture of circulatory endothelial progenitor cells and accelerated re-endothelialization of a bio-engineered stent in human ex vivo shunt and rabbit denudation model

    NARCIS (Netherlands)

    K. Larsen (Katarína); C. Cheng (Caroline (Ka Lai)); D. Tempel (Dennie); S. Parker (Sherry); S. Yazdani (Saami); W.K. den Dekker (Wijnand); H.J. Houtgraaf (Jaco); R. de Jong (Renate); S. Swager-ten Hoor (Stijn); E. Ligtenberg (Erik); S.R. Hanson (Stephen); R. Rowland (Steve); F. Kolodgie (Frank); P.W.J.C. Serruys (Patrick); R. Virmani (Renu); H.J. Duckers (Henricus)

    2012-01-01

    textabstractThe Genous™ Bio-engineered R™ stent (GS) aims to promote vascular healing by capture of circulatory endothelial progenitor cells (EPCs) to the surface of the stent struts, resulting in accelerated re-endothelialization. Here, we assessed the function of the GS in comparison to bare-metal

  17. Bioengineering targeted nanodrugs for hematologic malignancies: An innovation in pediatric oncology

    Science.gov (United States)

    Krishnan, Vinu

    Chemotherapy for pediatric cancers employs combinations of highly toxic drugs. This has achieved 5-year survival rates exceeding 90% in children treated for leukemia -- the most prominent form of pediatric cancer. However, delayed onset of harmful side effects in more than 60% of survivors result in death or low quality of life post therapy. This is primarily due to the non-specific effect of drugs on healthy dividing cells in a growing child. Nanomedicine has advanced tremendously to improve adult cancer therapy, but as yet has had minimal impact in pediatric oncology. There is a pressing need for innovative therapeutic strategies that can reduce life-threatening side effects caused by conventional chemotherapy in the clinic. Targeting chemotherapeutic agents specifically to leukemia cells may alleviate treatment-related toxicity in children. The research objective of this dissertation is to bioengineer and advance preclinically a novel nanotherapeutic approach that can specifically target and deliver drugs into leukemic cells. Dexamethasone (Dex) is one of the most commonly used chemotherapeutic drugs in treating pediatric leukemia. For the first part in this study, we encapsulated Dex in polymeric NPs and validated its anti-leukemic potential in vitro and in vivo. NPs with an average diameter of 110 nm were assembled from an amphiphilic block copolymer of poly(ethylene glycol) (PEG) and poly-caprolactone (PCL) bearing pendant cyclic ketals (ECT2). The blank NPs were nontoxic to cultured cells in vitro and to mice in vivo. Encapsulation of Dex into the NPs (Dex-NP) did not compromise the bioactivity of the drug. Dex-NPs induced glucocorticoid phosphorylation and showed cytotoxicity similar to free drug when treated with leukemic cells. Studies using NPs labeled with fluorescent dyes revealed leukemic cell surface binding and internalization. In vivo biodistribution studies showed NP accumulation in the liver and spleen with subsequent clearance of particles with

  18. Designing a Bioengine for Detection and Analysis of Base String on an Affected Sequence in High-Concentration Regions

    Directory of Open Access Journals (Sweden)

    Debnath Bhattacharyya

    2013-01-01

    Full Text Available We design an Algorithm for bioengine. As a program are enable optimal alignments searching between two sequences, the host sequence (normal plant as well as query sequence (virus. Searching for homologues has become a routine operation of biological sequences in 4 × 4 combination with different subsequence (word size. This program takes the advantage of the high degree of homology between such sequences to construct an alignment of the matching regions. There is a main aim which is to detect the overlapping reading frames. This program also enables to find out the highly infected colones selection highest matching region with minimum gap or mismatch zones and unique virus colones matches. This is a small, portable, interactive, front-end program intended to be used to find out the regions of matching between host sequence and query subsequences. All the operations are carried out in fraction of seconds, depending on the required task and on the sequence length.

  19. Designing a Bioengine for Detection and Analysis of Base String on an Affected Sequence in High-Concentration Regions

    Science.gov (United States)

    Mandal, Bijoy Kumar; Kim, Tai-hoon

    2013-01-01

    We design an Algorithm for bioengine. As a program are enable optimal alignments searching between two sequences, the host sequence (normal plant) as well as query sequence (virus). Searching for homologues has become a routine operation of biological sequences in 4 × 4 combination with different subsequence (word size). This program takes the advantage of the high degree of homology between such sequences to construct an alignment of the matching regions. There is a main aim which is to detect the overlapping reading frames. This program also enables to find out the highly infected colones selection highest matching region with minimum gap or mismatch zones and unique virus colones matches. This is a small, portable, interactive, front-end program intended to be used to find out the regions of matching between host sequence and query subsequences. All the operations are carried out in fraction of seconds, depending on the required task and on the sequence length. PMID:24000321

  20. Next Generation Respiratory Viral Vaccine System: Advanced and Emerging Bioengineered Human Lung Epithelia Model (HLEM) Organoid Technology

    Science.gov (United States)

    Goodwin, Thomas J.; Schneider, Sandra L.; MacIntosh, Victor; Gibbons, Thomas F.

    2010-01-01

    Acute respiratory infections, including pneumonia and influenza, are the S t" leading cause of United States and worldwide deaths. Newly emerging pathogens signaled the need for an advanced generation of vaccine technology.. Human bronchial-tracheal epithelial tissue was bioengineered to detect, identify, host and study the pathogenesis of acute respiratory viral disease. The 3-dimensional (3D) human lung epithelio-mesechymal tissue-like assemblies (HLEM TLAs) share characteristics with human respiratory epithelium: tight junctions, desmosomes, microvilli, functional markers villin, keratins and production of tissue mucin. Respiratory Syntial Virus (RSV) studies demonstrate viral growth kinetics and membrane bound glycoproteins up to day 20 post infection in the human lung-orgainoid infected cell system. Peak replication of RSV occurred on day 10 at 7 log10 particles forming units per ml/day. HLEM is an advanced virus vaccine model and biosentinel system for emergent viral infectious diseases to support DoD global surveillance and military readiness.

  1. Impact of mussel bioengineering on fine-grained sediment dynamics in a coastal lagoon: a numerical modelling investigation

    DEFF Research Database (Denmark)

    Forsberg, Pernille Louise; Lumborg, Ulrik; Bundgaard, Klavs

    2017-01-01

    Rødsand lagoon in southeast Denmark is a non-tidal coastal lagoon. It is home to a wide range of marine flora and fauna and part of the Natura 2000 network. An increase in turbidity through elevated levels of suspended sediment concentration (SSC) within the lagoon may affect the ecosystem health...... due to reduced light penetration. Increasing SSC levels within Rødsand lagoon could be caused by increasing storm intensity or by a sediment spill from dredging activities west of the lagoon in relation to the planned construction of the Fehmarnbelt fixed link between Denmark and Germany. The aim...... of the study was to investigate the impact of a mussel reef on sediment import and SSC in a semi-enclosed lagoon through the development of a bioengineering modelling application that makes it possible to include the filtrating effect of mussels in a numerical model of the lagoonal system. The numerical...

  2. Platelet-rich plasma enhances the integration of bioengineered cartilage with native tissue in an in vitro model.

    Science.gov (United States)

    Sermer, Corey; Kandel, Rita; Anderson, Jesse; Hurtig, Mark; Theodoropoulos, John

    2018-02-01

    Current therapies for cartilage repair can be limited by an inability of the repair tissue to integrate with host tissue. Thus, there is interest in developing approaches to enhance integration. We have previously shown that platelet-rich plasma (PRP) improves cartilage tissue formation. This raised the question as to whether PRP could promote cartilage integration. Chondrocytes were isolated from cartilage harvested from bovine joints, seeded on a porous bone substitute and grown in vitro to form an osteochondral-like implant. After 7 days, the biphasic construct was soaked in PRP for 30 min before implantation into the core of a donut-shaped biphasic explant of native cartilage and bone. Controls were not soaked in PRP. The implant-explant construct was cultured for 2-4 weeks. PRP-soaked bioengineered implants integrated with host tissue in 73% of samples, whereas controls only integrated in 19% of samples. The integration strength, as determined by a push-out test, was significantly increased in the PRP-soaked implant group (219 ± 35.4 kPa) compared with controls (72.0 ± 28.5 kPa). This correlated with an increase in glycosaminoglycan and collagen accumulation in the region of integration in the PRP-treated implant group, compared with untreated controls. Immunohistochemical studies revealed that the integration zone contained collagen type II and aggrecan. The cells at the zone of integration in the PRP-soaked group had a 3.5-fold increase in matrix metalloproteinase-13 gene expression compared with controls. These results suggest that PRP-soaked bioengineered cartilage implants may be a better approach for cartilage repair due to enhanced integration. Copyright © 2017 John Wiley & Sons, Ltd.

  3. The Challenges of Recombinant Endostatin in Clinical Application: Focus on the Different Expression Systems and Molecular Bioengineering

    Directory of Open Access Journals (Sweden)

    Abbas Mohajeri

    2017-04-01

    Full Text Available Angiogenesis plays an essential role in rapid growing and metastasis of the tumors. Inhibition of angiogenesis is a putative strategy for cancer therapy. Endostatin (Es is an attractive anti-angiogenesis protein with some clinical application challenges including; short half-life, instability in serum and requirement to high dosage. Therefore, production of recombinant endostatin (rEs is necessary in large scale. The production of rEs is difficult because of its structural properties and is high-cost. Therefore, this review focused on the different expression systems that involved in rEs production including; mammalian, baculovirus, yeast, and Escherichia coli (E. coli expression systems. The evaluating of the results of different expression systems declared that none of the mentioned systems can be considered to be generally superior to the other. Meanwhile with considering the advantages and disadvantage of E. coli expression system compared with other systems beside the molecular properties of Es, E. coli expression system can be a preferred expression system for expressing of the Es in large scale. Also, the molecular bioengineering and sustained release formulations that lead to improving of its stability and bioactivity will be discussed. Point mutation (P125A of Es, addition of RGD moiety or an additional zinc biding site to N-terminal of Es , fusing of Es to anti-HER2 IgG or heavy-chain of IgG, and finally loading of the endostar by PLGA and PEG- PLGA nanoparticles and gold nano-shell particles are the effective bioengineering methods to overcome to clinical changes of endostatin.

  4. Impact of mussel bioengineering on fine-grained sediment dynamics in a coastal lagoon: A numerical modelling investigation

    Science.gov (United States)

    Forsberg, Pernille L.; Lumborg, Ulrik; Bundgaard, Klavs; Ernstsen, Verner B.

    2017-12-01

    Rødsand lagoon in southeast Denmark is a non-tidal coastal lagoon. It is home to a wide range of marine flora and fauna and part of the Natura 2000 network. An increase in turbidity through elevated levels of suspended sediment concentration (SSC) within the lagoon may affect the ecosystem health due to reduced light penetration. Increasing SSC levels within Rødsand lagoon could be caused by increasing storm intensity or by a sediment spill from dredging activities west of the lagoon in relation to the planned construction of the Fehmarnbelt fixed link between Denmark and Germany. The aim of the study was to investigate the impact of a mussel reef on sediment import and SSC in a semi-enclosed lagoon through the development of a bioengineering modelling application that makes it possible to include the filtrating effect of mussels in a numerical model of the lagoonal system. The numerical implementation of an exterior mussel reef generated a reduction in the SSC in the vicinity of the reef, through the adjacent inlet and in the western part of the lagoon. The mussel reef reduced the sediment import to Rødsand lagoon by 13-22% and reduced the SSC within Rødsand lagoon by 5-9% depending on the filtration rate and the reef length. The results suggest that the implementation of a mussel reef has the potential to relieve the pressure of increasing turbidity levels within a semi-enclosed lagoonal system. However, further assessment and development of the bioengineering application and resulting ecosystem impacts are necessary prior to actual implementation.

  5. A tribute to Dr Willem J. Kolff: innovative inventor, physician, scientist, bioengineer, mentor, and significant contributor to modern cardiovascular surgical and anesthetic practice.

    Science.gov (United States)

    Stanley, Theodore H

    2013-06-01

    Dr Willem J. Kolff was surely one of the greatest inventors/physicians/scientists/bioengineers of the last few hundred years. He was knighted (Commander of the Order of Oranje-Nassau) in 1970 by Queen Juliana of the Netherlands. In 1990, Life magazine published a list of its own 100 most important figures of the 20th century. Kolff stood in 99th place as the Father of Artificial Organs. Dr Kolff forged a path of innovative thinking and creativity that has had a huge impact on the quality of human life. His contributions to the development of the artificial kidney and dialysis, the heart-lung machine, the membrane oxygenator, potassium arrest of the heart, the AH, mechanical cardiac assistance, and other artificial organs, and his support and mentoring of hundreds to thousands of anesthesiologists, surgeons, and bioengineers throughout the world, have had a significant impact on anesthesiology and the medical community.

  6. Immobilized enzymes to convert N-sulfo, N-acetyl heparosan to a critical intermediate in the production of bioengineered heparin.

    Science.gov (United States)

    Xiong, Jian; Bhaskar, Ujjwal; Li, Guoyun; Fu, Li; Li, Lingyun; Zhang, Fuming; Dordick, Jonathan S; Linhardt, Robert J

    2013-09-10

    Heparin is a critically important anticoagulant drug that is prepared from pig intestine. In 2007-2008, there was a crisis in the heparin market when the raw material was adulterated with the toxic polysaccharide, oversulfated chondroitin sulfate, which was associated with 100 deaths in the U.S. alone. As the result of this crisis, our laboratory and others have been actively pursuing alternative sources for this critical drug, including synthetic heparins and bioengineered heparin. In assessing the bioengineering processing costs it has become clear that the use of both enzyme-catalyzed cofactor recycling and enzyme immobilization will be needed for commercialization. In the current study, we examine the use of immobilization of C₅-epimerase and 2-O-sulfotransferase involved in the first enzymatic step in the bioengineered heparin process, as well as arylsulfotransferase-IV involved in cofactor recycling in all three enzymatic steps. We report the successful immobilization of all three enzymes and their use in converting N-sulfo, N-acetyl heparosan into N-sulfo, N-acetyl 2-O-sulfo heparin. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. Reabilitation of degraded area by erosion, using soil bioengineering techniques in Bacanga river basin, Sao Luis City - Maranhao State, Brazil.

    Science.gov (United States)

    Teixeira Guerra, A. J.; Rodrigues Bezerra, J. F.; da Mota Lima, L. D.; Silva Mendonça, J. K.; Vieira Souza, U. D.; Teixeira Guerra, T.

    2009-04-01

    The aim of this paper is to assess the stages of rehabilitation of a degraded site by erosion, in Salina/Sacavém district, São Luís City, considering geomorphologic characteristics and soil bioengineering techniques. This technique has been applied in different situations to rehabilitate degraded areas, with positive results from the use of biodegradable materials (e.g. vegetal fibres, wooden stakes and re-vegetation). These techniques stabilize the soil at low cost and improve the environment. Bioengineering involves the planned and strategic application of selected materials, involving biodegradable materials, often in combination with 'hard engineering' structures constructed from stone, concrete and steel. The settlement of São Luís was established in 1612 and has evolved in distinct phases. Rapid urban growth was associated with industrialization in the second half of the 18th Century. Rapid population and urban growth has intensified problems, compounded by poor planning and improper soil use. São Luís, like many other Brazilian cities, has experienced rapid population growth in recent decades, which has created a series of socio-economic and environmental problems, including accelerated soil erosion. Sacavém is one of these communities where natural and human factors contribute to the severe gully erosion. The local lithology is mainly Tertiary sandstones and, to a lesser extent, shales, argillites and siltstones, all of which belong to the Barreiras Formation. Weathering on these rocks produces erodible soils, including lithosols, latosols, concretionary red/yellow clay soils and concretionary plinthosols. Thus, erodible soils and regolith are subject to high erosion rates, especially on steeper slopes subject to additional human interventions. Furthermore, although regional slopes are quite gentle, there is localized high relative relief. Sacavém vegetation, in the gullied area, consists of brushwood. Secondary mixed forest and brushwood are the

  8. Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4-loaded hydrogel directs hard tissue bioengineering.

    Science.gov (United States)

    Diniz, Ivana M A; Carreira, Ana C O; Sipert, Carla R; Uehara, Cindi M; Moreira, Maria S N; Freire, Laila; Pelissari, Cibele; Kossugue, Patrícia M; de Araújo, Daniele R; Sogayar, Mari C; Marques, Márcia M

    2018-06-01

    Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic ® F-127) loaded with rhBMP4 and then photoactivated. PBM improved MSCs self-renewal and survival upon encapsulation in the Pluronic ® F-127. In the presence of rhBMP4, cell odonto/osteogenic differentiation was premature and markedly improved in the photoactivated MSCs. An in vivo calvarial critical sized defect model demonstrated significant increase in bone formation after PBM treatment. Finally, a balance in the reactive oxygen species levels may be related to the favorable results of PBM and rhBMP4 association. PBM may act in synergism with rhBMP4 and is a promise candidate to direct and accelerate hard tissue bioengineering. © 2017 Wiley Periodicals, Inc.

  9. A Bioengineered Three-Dimensional Cell Culture Platform Integrated with Microfluidics To Address Antimicrobial Resistance in Tuberculosis

    Directory of Open Access Journals (Sweden)

    Magdalena K. Bielecka

    2017-02-01

    Full Text Available Antimicrobial resistance presents one of the most significant threats to human health, with the emergence of totally drug-resistant organisms. We have combined bioengineering, genetically modified bacteria, longitudinal readouts, and fluidics to develop a transformative platform to address the drug development bottleneck, utilizing Mycobacterium tuberculosis as the model organism. We generated microspheres incorporating virulent reporter bacilli, primary human cells, and an extracellular matrix by using bioelectrospray methodology. Granulomas form within the three-dimensional matrix, and mycobacterial stress genes are upregulated. Pyrazinamide, a vital first-line antibiotic for treating human tuberculosis, kills M. tuberculosis in a three-dimensional culture but not in a standard two-dimensional culture or Middlebrook 7H9 broth, demonstrating that antibiotic sensitivity within microspheres reflects conditions in patients. We then performed pharmacokinetic modeling by combining the microsphere system with a microfluidic plate and demonstrated that we can model the effect of dynamic antibiotic concentrations on mycobacterial killing. The microsphere system is highly tractable, permitting variation of cell content, the extracellular matrix, sphere size, the infectious dose, and the surrounding medium with the potential to address a wide array of human infections and the threat of antimicrobial resistance.

  10. Bioinformatic analysis of the distribution of inorganic carbon transporters and prospective targets for bioengineering to increase Ci uptake by cyanobacteria.

    Science.gov (United States)

    Gaudana, Sandeep B; Zarzycki, Jan; Moparthi, Vamsi K; Kerfeld, Cheryl A

    2015-10-01

    Cyanobacteria have evolved a carbon-concentrating mechanism (CCM) which has enabled them to inhabit diverse environments encompassing a range of inorganic carbon (Ci: [Formula: see text] and CO2) concentrations. Several uptake systems facilitate inorganic carbon accumulation in the cell, which can in turn be fixed by ribulose 1,5-bisphosphate carboxylase/oxygenase. Here we survey the distribution of genes encoding known Ci uptake systems in cyanobacterial genomes and, using a pfam- and gene context-based approach, identify in the marine (alpha) cyanobacteria a heretofore unrecognized number of putative counterparts to the well-known Ci transporters of beta cyanobacteria. In addition, our analysis shows that there is a huge repertoire of transport systems in cyanobacteria of unknown function, many with homology to characterized Ci transporters. These can be viewed as prospective targets for conversion into ancillary Ci transporters through bioengineering. Increasing intracellular Ci concentration coupled with efforts to increase carbon fixation will be beneficial for the downstream conversion of fixed carbon into value-added products including biofuels. In addition to CCM transporter homologs, we also survey the occurrence of rhodopsin homologs in cyanobacteria, including bacteriorhodopsin, a class of retinal-binding, light-activated proton pumps. Because they are light driven and because of the apparent ease of altering their ion selectivity, we use this as an example of re-purposing an endogenous transporter for the augmentation of Ci uptake by cyanobacteria and potentially chloroplasts.

  11. Fabrication of Collagen Gel Hollow Fibers by Covalent Cross-Linking for Construction of Bioengineering Renal Tubules.

    Science.gov (United States)

    Shen, Chong; Zhang, Guoliang; Wang, Qichen; Meng, Qin

    2015-09-09

    Collagen, the most used natural biomacromolecule, has been extensively utilized to make scaffolds for cell cultures in tissue engineering, but has never been fabricated into the configuration of a hollow fiber (HF) for cell culture due to its poor mechanical properties. In this study, renal tubular cell-laden collagen hollow fiber (Col HF) was fabricated by dissolving sacrificial Ca-alginate cores from collagen shells strengthened by carbodiimide cross-linking. The inner/outer diameters of the Col HF were precisely controlled by the flow rates of core alginate/shell collagen solution in the microfluidic device. As found, the renal tubular cells self-assembled into renal tubules with diameters of 50-200 μm post to the culture in Col HF for 10 days. According to the 3D reconstructed confocal images or HE staining, the renal cells appeared as a tight tubular monolayer on the Col HF inner surface, sustaining more 3D cell morphology than the cell layer on the 2D flat collagen gel surface. Moreover, compared with the cultures in either a Transwell or polymer HF membrane, the renal tubules in Col HF exhibited at least 1-fold higher activity on brush border enzymes of alkaline phosphatase and γ-glutamyltransferase, consistent with their gene expressions. The enhancement occurred similarly on multidrug resistance protein 2 and glucose uptake. Such bioengineered renal tubules in Col HF will present great potential as alternatives to synthetic HF in both clinical use and pharmaceutical investigation.

  12. Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.

    Science.gov (United States)

    Sood, Disha; Chwalek, Karolina; Stuntz, Emily; Pouli, Dimitra; Du, Chuang; Tang-Schomer, Min; Georgakoudi, Irene; Black, Lauren D; Kaplan, David L

    2016-01-01

    The extracellular matrix (ECM) constituting up to 20% of the organ volume is a significant component of the brain due to its instructive role in the compartmentalization of functional microdomains in every brain structure. The composition, quantity and structure of ECM changes dramatically during the development of an organism greatly contributing to the remarkably sophisticated architecture and function of the brain. Since fetal brain is highly plastic, we hypothesize that the fetal brain ECM may contain cues promoting neural growth and differentiation, highly desired in regenerative medicine. Thus, we studied the effect of brain-derived fetal and adult ECM complemented with matricellular proteins on cortical neurons using in vitro 3D bioengineered model of cortical brain tissue. The tested parameters included neuronal network density, cell viability, calcium signaling and electrophysiology. Both, adult and fetal brain ECM as well as matricellular proteins significantly improved neural network formation as compared to single component, collagen I matrix. Additionally, the brain ECM improved cell viability and lowered glutamate release. The fetal brain ECM induced superior neural network formation, calcium signaling and spontaneous spiking activity over adult brain ECM. This study highlights the difference in the neuroinductive properties of fetal and adult brain ECM and suggests that delineating the basis for this divergence may have implications for regenerative medicine.

  13. Whistleblower Protection

    Science.gov (United States)

    The Whistleblower Protection Enhancement Act of 2012 (WPA) and the Whistleblower Protection Act of 1989 Enhanced by the Act of 2012 provides protection rights for Federal employees against retaliation for whistleblowing activities.

  14. Bioengineering vascularized tissue constructs using an injectable cell-laden enzymatically crosslinked collagen hydrogel derived from dermal extracellular matrix.

    Science.gov (United States)

    Kuo, Kuan-Chih; Lin, Ruei-Zeng; Tien, Han-Wen; Wu, Pei-Yun; Li, Yen-Cheng; Melero-Martin, Juan M; Chen, Ying-Chieh

    2015-11-01

    -Ph hydrogel resulted in not only improving the long-term differentiation of transplanted MSCs into mineralized osteoblasts, but the collagen-Ph hydrogel also improved an increased of adipocytes within the vascularized bioengineered tissue in a mouse after 1 month of implantation. We reported a method for preparing autologous extracellular matrix scaffolds, murine collagen-Ph hydrogels, and demonstrated its suitability for use in supporting human progenitor cell-based formation of 3D vascular networks in vitro and in vivo. Results showed extensive human vascular networks can be generated within 7 days, engineered vascular density inside collagen-Ph constructs can be manipulated through refinable mechanical properties and proteolytic degradability, and these networks can form functional anastomoses with existing vasculature to further support the survival of host muscle tissues. Moreover, optimized conditions of cell-laden collagen-Ph hydrogel resulted in not only improving the long-term differentiation of transplanted MSCs into mineralized osteoblasts, but the collagen-Ph hydrogel also improved an increased of adipocytes within the vascularized bioengineered tissue in a mouse. Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

  15. Low cost and open source multi-fluorescence imaging system for teaching and research in biology and bioengineering.

    Directory of Open Access Journals (Sweden)

    Isaac Nuñez

    Full Text Available The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates. We developed a set of genetic components (e.g. promoters, coding sequences, terminators and vectors following the standard framework of Golden Gate, which allowed the fabrication of genetic constructs in a combinatorial, low cost and robust manner. In order to provide simultaneous imaging of multiple wavelength signals, we screened a series of long stokes shift fluorescent proteins that could be combined with cyan/green fluorescent proteins. We found CyOFP1, mBeRFP and sfGFP to be the most compatible set for 3-channel fluorescent imaging. We developed open source Python code to operate the hardware to run time-lapse experiments with automated control of illumination and camera and a Python module to analyze data and extract meaningful biological information. To demonstrate the potential application of this integral system, we tested its performance on a diverse range of imaging assays often used in disciplines such as microbial ecology, microbiology and synthetic biology. We also assessed its potential use in a high school environment to teach biology, hardware design, optics, and programming. Together, these results demonstrate the successful integration of open source hardware, software, genetic resources and customizable manufacturing to obtain a powerful, low cost and robust system for education, scientific research and bioengineering. All the resources developed here

  16. Low cost and open source multi-fluorescence imaging system for teaching and research in biology and bioengineering.

    Science.gov (United States)

    Nuñez, Isaac; Matute, Tamara; Herrera, Roberto; Keymer, Juan; Marzullo, Timothy; Rudge, Timothy; Federici, Fernán

    2017-01-01

    The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates. We developed a set of genetic components (e.g. promoters, coding sequences, terminators) and vectors following the standard framework of Golden Gate, which allowed the fabrication of genetic constructs in a combinatorial, low cost and robust manner. In order to provide simultaneous imaging of multiple wavelength signals, we screened a series of long stokes shift fluorescent proteins that could be combined with cyan/green fluorescent proteins. We found CyOFP1, mBeRFP and sfGFP to be the most compatible set for 3-channel fluorescent imaging. We developed open source Python code to operate the hardware to run time-lapse experiments with automated control of illumination and camera and a Python module to analyze data and extract meaningful biological information. To demonstrate the potential application of this integral system, we tested its performance on a diverse range of imaging assays often used in disciplines such as microbial ecology, microbiology and synthetic biology. We also assessed its potential use in a high school environment to teach biology, hardware design, optics, and programming. Together, these results demonstrate the successful integration of open source hardware, software, genetic resources and customizable manufacturing to obtain a powerful, low cost and robust system for education, scientific research and bioengineering. All the resources developed here are available under

  17. A bioengineered nisin derivative, M21A, in combination with food grade additives eradicates biofilms of Listeria monocytogenes.

    Directory of Open Access Journals (Sweden)

    Lorraine Anne Draper

    2016-11-01

    Full Text Available The burden of foodborne disease has large economic and social consequences worldwide. Despite strict regulations, a number of pathogens persist within the food environment, which is greatly contributed to by a build-up of resistance mechanisms and also through the formation of biofilms. Biofilms have been shown to be highly resistant to a number of antimicrobials and can be extremely difficult to remove once they are established. In parallel, the growing concern of consumers regarding the use of chemically derived antimicrobials within food has led to a drive towards more natural products. As a consequence, the use of naturally derived antimicrobials has become of particular interest. In this study we investigated the efficacy of nisin A and its bioengineered derivative M21A in combination with food grade additives to treat biofilms of a representative strain of Listeria monocytogenes. Investigations revealed the enhanced antimicrobial effects, in liquid culture, of M21A in combination with citric acid or cinnamaldehyde over its wild type nisin A counterpart. Subsequently, an investigation was conducted into the effects of these combinations on an established biofilm of the same strain. Nisin M21A (0.1 µg/ml alone or in combination with cinnamaldehyde (35 µg/ml or citric acid (175 µg/ml performed significantly better than combinations involving nisin A. All combinations of M21A with either citric acid or cinnamaldehyde eradicated the L. monocytogenes biofilm (in relation to a non-biofilm control. We conclude that M21A in combination with available food additives could further enhance the antimicrobial treatment of biofilms within the food industry, simply by substituting nisin A with M21A in current commercial products such as Nisaplin (Danisco, DuPont.

  18. Radiation protection

    International Nuclear Information System (INIS)

    Koelzer, W.

    1975-01-01

    Physical and radiological terms, quantities, and units. Basic principles of radiation protection (ICRP, IAEA, EURATOM, FRG). Biological effects of ionizing radiation. Objectives of practical radiation protection. (HP) [de

  19. Reprogramming somatic cell differentiation and the Hayflick Limit: contrasting two modern molecular bioengineering aims and their impact on the future of mankind.

    Science.gov (United States)

    Sills, E S; Takeuchi, T; Rosenwaks, Z; Palermo, G D

    2001-08-01

    The molecular biology of human cloning and aging research depend on the closely related laboratory techniques supported by a thorough understanding of cell-signaling processes. Unfortunately, the link between these two research fields has received only marginal attention in the lay press. Cloning is possible when somatic cell differentiation is successfully reprogrammed, and clinical control of cellular senescence depends on a proper reconfiguration of the predetermined number of divisions permitted during the cell life-cycle (the so-called "Hayflick Limit"). In this paper, we discuss these two concepts and compare the impact likely to be associated with bioengineering studies that facilitate both human cloning and longevity therapy.

  20. Proceedings of the 1. National Forum of Science and Technology on Health; 13. Brazilian Congress on Biomedical Engineering; 4. Brazilian Congress of Physicists on Medicine; Brazilian Meeting on Biology and Nuclear Medicine; Brazilian Meeting on Radiological Protection

    International Nuclear Information System (INIS)

    Costa, E.T.; Martins, H.L.; Muehlen, S.S.; Rockman, T.M.B.

    1992-01-01

    This 1. National Forum of Science and Technology on Health presents works of several scientific institutions, including topics on bioengineering; modelling and simulation; sensors and transducers; ultrasonic on medicine; instrumentation processing of signs and medical images; biomedical informatics and clinical software; engineering of rehabilitation; bio-materials and bio-mechanical; clinical engineering; in vivo and in vitro nuclear medicine; radioisotope production and utilization; radiology; radiology protection and dosimetry; radiotherapy; evaluation of technology on health and education. (C.G.C.)

  1. Physical protection

    International Nuclear Information System (INIS)

    Myers, D.A.

    1989-01-01

    Physical protection is defined and its function in relation to other functions of a State System of Accounting for and Control of Nuclear Materials is described. The need for a uniform minimum international standard for physical protection as well as the need for international cooperation in physical protection is emphasized. The IAEA's INFCIRC/225/Rev. 1 (Annex 1) is reviewed. The Convention on the Physical Protection of Nuclear Material (Annex 2) is discussed. Photographs show examples of typical physical protection technology (Annex 3)

  2. Diplomatic Protection

    OpenAIRE

    Režná, Jana

    2006-01-01

    Final thesis Topic: Diplomatic protection Thesis supervisor: JUDr. Vladimír Balaš, CSc. Student: Marek Čermák Thesis on the topic of diplomatic protection deals with the granting of exercise of diplomatic protection by the states and is divided into seven chapters which follow each other. The first chapter describes the diplomatic protection and its historical foundations. The second chapter focuses on the possibility of exercise of diplomatic protection in respect of natural persons and the ...

  3. PENGARUH PERILAKU MASYARAKAT YANG BERMUKIM DI KAWASAN BANTARAN SUNGAI TERHADAP PENURUNAN KUALITAS AIR SUNGAI KARANG ANYAR KOTA TARAKAN (Influence of The Behavior of Citizens Residing in Riverbanks to The Decrease of Water Quality in The River of Karang

    Directory of Open Access Journals (Sweden)

    Ira Puspita

    2016-07-01

    The data of environmental status of Tarakan City states that water quality of Karang Anyar’s river for COD, ammonia and TSS paremeters from year 2010-2013 are above the government regulation for quality standard. The decreasing of Karang Anyar’s river water quality are influenced by the behavior of regional society along the riverbanks. Research methods used in this research is the combination between quantitative and qualitative methods. The result of this research are the disposal of domestic waste water directly into the river suspected in affecting the parameters COD and ammonia exceeded the quality standard because waste water contains foam of soap sources from waste water of wash. The disposal of domestic waste water from cooking residue in affecting ammonia parameters exceeded the quality standard. The behavior of livestock chicken was suspected affecting ammonia parameter in exceeding the quality standard because of chicken’s feces have been decomposed. Mining soil behavior from hills or mountains suspected not affecting TSS parameter because of climate influenced. The behaviour of sand river’s mining suspected affecting ammonia parameter and exceeding the quality standard but did not affect TSS parameter because the activities were not daily and the miners depending on rain level and sand volume. The conclusion of this research are the not all behavior of regional society along the riverbanks affect the decreasing of Karang Anyar’s river water quality.

  4. Human immature dental pulp stem cells (hIDPSCs), their application to cell therapy and bioengineering: an analysis by systematic revision of the last decade of literature.

    Science.gov (United States)

    de Souza, Priscilla Vianna; Alves, Fabiana Bucholdz Teixeira; Costa Ayub, Cristina Lucia Sant'Ana; de Miranda Soares, Maria Albertina; Gomes, Jose Rosa

    2013-12-01

    During recent years, attention has been given to the potential of therapeutic approaches using stem cells obtained from dental pulp tissue. The aim of this study, therefore, was to give an overview of the papers produced during the last 10 years that have described the use of stem cells obtained from human deciduous teeth in cell therapy or bioengineering. The PubMed database was investigated from January 2002 until July 2011 and the papers published during this period were analyzed according to criteria previously established, using the methodology of systematic review. The measurements were done using "stem cell" as the primary keyword, and "human deciduous teeth dental pulp cell" and "human exfoliated deciduous teeth" as the secondary keywords. Four hundred and seventy-five papers were found. The first screening resulted in 276 papers, from which 84 papers were selected. However, only 11 of them attained the aim proposed in our approach. There were few scientific studies related to direct therapeutic application using stem cells of human deciduous teeth and none of them had been applied to humans. However, the results indicated important and promising applications of the pulp stem-cells in cell therapy and bioengineering as demonstrated by studies in animal models of muscular dystrophy, Parkison's disease, and lupus erythematosus. Copyright © 2013 Wiley Periodicals, Inc.

  5. The Role of Inorganic Polyphosphates in the Formation of Bioengineered Cartilage Incorporating a Zone of Calcified Cartilage In Vitro

    Science.gov (United States)

    St-Pierre, Jean-Philippe

    The development of bioengineered cartilage for replacement of damaged articular cartilage has gained momentum in recent years. One such approach has been developed in the Kandel lab, whereby cartilage is formed by seeding primary articular chondrocytes on the top surface of a porous biodegradable calcium polyphosphate (CPP) bone substitute, permitting anchorage of the tissue within the pores of the substrate; however, the interfacial shear properties of the tissue-substrate interface of these biphasic constructs are 1 to 2 orders of magnitude lower than the native cartilage-subchondral bone interface. To overcome this limitation, a strategy was devised to generate a zone of calcified cartilage (ZCC), thereby mimicking the native architecture of the osteochondral junction; however, the ZCC was located slightly above the cartilage-CPP interface. Thus, it was hypothesized that polyphosphate released from the CPP substrate and accumulating in the tissue inhibits the formation of the ZCC at the tissue-substrate interface. Based on this information, a strategy was devised to generate biphasic constructs incorporating a properly located ZCC. This approach involved the application of a thin calcium phosphate film to the surfaces of porous CPP via a sol-gel procedure, thereby limiting the accumulation of polyphosphate in the cartilaginous tissue. This modification to the substrate surface did not negatively impact the quality of the in vitro-formed cartilage tissue or the ZCC. Interfacial shear testing of biphasic constructs demonstrated significantly improved interfacial shear properties in the presence of a properly located ZCC. These studies also led to the observation that chondrocytes produce endogenous polyphosphate and that its levels in deep zone cartilage appear inversely related to mineral deposition within the tissue. Using an in vitro model of cartilage calcification, it was demonstrated that polyphosphate levels are modulated in part by the inhibitory effects

  6. Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II

    Directory of Open Access Journals (Sweden)

    Gerstenhaber JA

    2017-11-01

    Full Text Available Jonathan A Gerstenhaber,1,* Frank C Barone,2,* Cezary Marcinkiewicz,1,3 Jie Li,2 Aaron O Shiloh,4 Mark Sternberg,3 Peter I Lelkes,1,* Giora Feuerstein1,3,* 1Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA, 2Department of Neurology, State University of New York Downstate Medical Center, Brooklyn, NY, 3Debina Diagnostic Inc., Newtown Square, 4Diagnostic Imaging, Inc., Philadelphia, PA, USA *These authors contributed equally to this work Abstract: The aim of this feasibility study was to test the ability of fluorescent nanodiamond particles (F-NDP covalently conjugated with bitistatin (F-NDP-Bit to detect vascular blood clots in vivo using extracorporeal near-infrared (NIR imaging. Specifically, we compared NIR fluorescence properties of F-NDP with N-V (F-NDPNV and N-V-N color centers and sizes (100–10,000 nm. Optimal NIR fluorescence and tissue penetration across biological tissues (rat skin, porcine axillary veins, and skin was obtained for F-NDPNV with a mean diameter of 700 nm. Intravital imaging (using in vivo imaging system [IVIS] in vitro revealed that F-NDPNV-loaded glass capillaries could be detected across 6 mm of rat red-muscle barrier and 12 mm of porcine skin, which equals the average vertical distance of a human carotid artery bifurcation from the surface of the adjacent skin (14 mm. In vivo, feasibility was demonstrated in a rat model of occlusive blood clots generated using FeCl3 in the carotid artery bifurcation. Following systemic infusions of F-NDPNV-Bit (3 or 15 mg/kg via the external carotid artery or femoral vein (N=3, presence of the particles in the thrombi was confirmed both in situ via IVIS, and ex vivo via confocal imaging. The presence of F-NDPNV in the vascular clots was further confirmed by direct counting of fluorescent particles extracted from clots following tissue solubilization. Our data suggest that F-NDPNV-Bit associate with vascular blood clots, presumably by binding

  7. Identifying new lignin bioengineering targets: 1. Monolignol-substitute impacts on lignin formation and cell wall fermentability

    Directory of Open Access Journals (Sweden)

    Lu Fachuang

    2010-06-01

    Full Text Available Abstract Background Recent discoveries highlighting the metabolic malleability of plant lignification indicate that lignin can be engineered to dramatically alter its composition and properties. Current plant biotechnology efforts are primarily aimed at manipulating the biosynthesis of normal monolignols, but in the future apoplastic targeting of phenolics from other metabolic pathways may provide new approaches for designing lignins that are less inhibitory toward the enzymatic hydrolysis of structural polysaccharides, both with and without biomass pretreatment. To identify promising new avenues for lignin bioengineering, we artificially lignified cell walls from maize cell suspensions with various combinations of normal monolignols (coniferyl and sinapyl alcohols plus a variety of phenolic monolignol substitutes. Cell walls were then incubated in vitro with anaerobic rumen microflora to assess the potential impact of lignin modifications on the enzymatic degradability of fibrous crops used for ruminant livestock or biofuel production. Results In the absence of anatomical constraints to digestion, lignification with normal monolignols hindered both the rate and extent of cell wall hydrolysis by rumen microflora. Inclusion of methyl caffeate, caffeoylquinic acid, or feruloylquinic acid with monolignols considerably depressed lignin formation and strikingly improved the degradability of cell walls. In contrast, dihydroconiferyl alcohol, guaiacyl glycerol, epicatechin, epigallocatechin, and epigallocatechin gallate readily formed copolymer-lignins with normal monolignols; cell wall degradability was moderately enhanced by greater hydroxylation or 1,2,3-triol functionality. Mono- or diferuloyl esters with various aliphatic or polyol groups readily copolymerized with monolignols, but in some cases they accelerated inactivation of wall-bound peroxidase and reduced lignification; cell wall degradability was influenced by lignin content and the degree

  8. Environmental protection

    International Nuclear Information System (INIS)

    Klinda, J.; Lieskovska, Z.

    1998-01-01

    In this chapter environmental protection in the Slovak Republic in 1997 are reviewed. The economics of environmental protection, state budget, Slovak state environmental fund, economic instruments, environmental laws, environmental impact assessment, environmental management systems, and environmental education are presented

  9. River-bed erosion due to changing boundary conditions: performance of a protective measure

    Directory of Open Access Journals (Sweden)

    D. Termini

    2014-09-01

    Full Text Available Due to the introduction of man-made sediment barriers along a river, the amount of sediment load entering the downstream river reach is different to that leaving the reach, and erosion processes occur downstream of the barrier itself. Designers are often required to take into account the scouring process and to include adequate protective measures against the local scour. This paper addresses the performance of bio-engineering protective measures against the erosion process. In particular, a green carpet, realized with real flexible vegetation, has been used as the protective measure against erosion processes downstream of a rigid bed. Analyses are based on experimental work carried out in a straight channel constructed at the laboratory of the Dipartimento di Ingegneria Civile, Ambientale, Aereospaziale, dei Materiali, Palermo University (Italy.

  10. Protective relay

    International Nuclear Information System (INIS)

    Lim, Mu Ji; Jung, Hae Sang

    1974-10-01

    This book is divided into two chapters, which deals with protective relay. The first chapter deals with the basic knowledge of relay on development of relay, classification of protective relay, rating of protective relay general structure of protective relay, detecting of ground protection, about point of contact, operating relay and trip relaying. The second chapter is about structure and explanation of relay on classification by structure such as motor type and moving-coil type, explanation of other relays over current relay, over voltage relay, short voltage relay, relay for power, relay for direction, test of over voltage relay, test of short voltage relay and test of directional circuit relay.

  11. Protecting knowledge

    DEFF Research Database (Denmark)

    Sofka, Wolfgang; de Faria, Pedro; Shehu, Edlira

    2018-01-01

    Most firms use secrecy to protect their knowledge from potential imitators. However, the theoretical foundations for secrecy have not been well explored. We extend knowledge protection literature and propose theoretical mechanisms explaining how information visibility influences the importance...... of secrecy as a knowledge protection instrument. Building on mechanisms from information economics and signaling theory, we postulate that secrecy is more important for protecting knowledge for firms that have legal requirements to reveal information to shareholders. Furthermore, we argue that this effect...... and a firm's investment in fixed assets. Our findings inform both academics and managers on how firms balance information disclosure requirements with the use of secrecy as a knowledge protection instrument....

  12. Update on Bioengineering.

    Science.gov (United States)

    Basta, Nicholas

    1986-01-01

    Discusses the human, plant and animal, and industrial dimensions of biotechnology, focusing on current uses in such fields as: (1) genetics; (2) electronics; (3) prosthetics; (4) drugs; (5) agriculture; and (6) the environment. (JN)

  13. Starch Bioengineering in Barley

    DEFF Research Database (Denmark)

    Shaik, Shahnoor Sultana

    , the effects of engineering high levels of phosphate and amylose content on starch physico-chemical properties were evaluated by various biochemical and morphological studies. As a result, a substantial increase of 10-fold phosphate content and ~99% amylose content with high-resistant starch was observed...... in storage reserve accumulation, metabolite accumulation in AO but no significant differences were observed in HP compared to WT. Scanning electron microscopy and confocal microscopy revealed the details in topography and internal structures of the starch granules in these lines. The results demonstrated......Starch represents the most important carbohydrate used for food and feed purposes. Increasingly, it is also used as a renewable raw material, as a source of biofuel, and for many different industrial applications. Progress in understanding starch biosynthesis, and investigations of the genes...

  14. Bioengineered riboflavin in nanotechnology

    NARCIS (Netherlands)

    Beztsinna, N; Solé, M; Taib, N; Bestel, I

    Riboflavin (RF) is an essential water-soluble vitamin with unique biological and physicochemical properties such as transporterspecific cell internalization, implication in redox reactions, fluorescence and photosensitizing. Due to these features RF attracted researchers in various fields from

  15. Tibiotalar torsion: bioengineering paradigm.

    Science.gov (United States)

    Michele, A A; Nielsen, P M

    1976-10-01

    1. Medial tibiotalar torsion is the most common disorder peculiar to mankind. 2. The pathogonomic findings are (a) an axial medially rotated and adducted distal third of the shaft of the tibia, (b) the plafond of the tibia with its mortise containing the "track-bound" talus, which is deflected strongly toward the tibial side, (c) an exaggerated midtarsal equinus, (d) ostensible restriction of dorsiflexion of the hindfoot against the tibia, (e) mild separation of the distal tibiofibular articulation, and (f) forward displacement of the gravitational axis to the naviculocunei-form joint. 3. Faulty leg crossing in utero resulting in an abnormal pelvofemoral-tibial design is discussed and its important consequences in the vulnerable 40 per cent of the population are emphasized. 4. The kinesiomechanics of the leg, ankle and foot is reviewed. 5. The radiographic parameters of medial tibiotalar torsion are presented, as well as the multiple facets of the clinical examination. 6. Methods of treatment depending on age and severity of the disorder are recommended. Surgery, detortional casts, and corrective footwear are discussed. Shoes presently available are inadequate for tibiotalar torsion and therefore engineering principles must be applied in the design and construction of all footwear, including sneakers and sportswear. This can be done only if the pathological biomechanics of this group of disorders is recognized. Biplane proximal tibial osteotomy is recommended in refractory cases, especially when tibiotalar torsion is demonstrated. 7. After 30 years of experience, the author finds that results with these patients have been uniformly good to excellent, depending on age and mode of treatment. 8. In medial tibiotalar torsion, the consequent adaptive changes are readily observed, but rarely are they recognized as the inevitable sequelae of medial tibiotalar torsion. 9. Adaptive compensating disorders are identified and their mechanism described. 10. The management of torsional and static disorders and adaptations of the leg, foot, and knee should not continue on an empirical basis. Recognition of recently presented parameters of the lower limbs in the vulnerable "40 percenters" permits the use of sound engineering principles in both conservative and operative correction of these disorders.

  16. Radiation bioengineering; Bioinzynieria radiacyjna

    Energy Technology Data Exchange (ETDEWEB)

    Rosiak, J M [Politechnika Lodzka, Lodz (Poland). Inst. Techniki Radiacynej

    1997-10-01

    Radiation processing for modification of different properties of materials being designed for medical use have been described. Especially the polymers as very often used for medical equipment production have been modified by radiation. The different medical applications of biomaterials based on radiation modified polymers have been presented. 13 refs.

  17. Protocol for a cluster randomised stepped wedge trial assessing the impact of a community-level hygiene intervention and a water intervention using riverbank filtration technology on diarrhoeal prevalence in India.

    Science.gov (United States)

    McGuinness, Sarah L; O'Toole, Joanne E; Boving, Thomas B; Forbes, Andrew B; Sinclair, Martha; Gautam, Sumit K; Leder, Karin

    2017-03-17

    Diarrhoea is a leading cause of death globally, mostly occurring as a result of insufficient or unsafe water supplies, inadequate sanitation and poor hygiene. Our study aims to investigate the impact of a community-level hygiene education program and a water quality intervention using riverbank filtration (RBF) technology on diarrhoeal prevalence. We have designed a stepped wedge cluster randomised trial to estimate the health impacts of our intervention in 4 rural villages in Karnataka, India. At baseline, surveys will be conducted in all villages, and householders will receive hygiene education. New pipelines, water storage tanks and taps will then be installed at accessible locations in each village and untreated piped river water will be supplied. A subsequent survey will evaluate the impact of hygiene education combined with improved access to greater water volumes for hygiene and drinking purposes (improved water quantity). Villages will then be randomly ordered and RBF-treated water (improved water quality) will be sequentially introduced into the 4 villages in a stepwise manner, with administration of surveys at each time point. The primary outcome is a 7-day period prevalence of self-reported diarrhoea. Secondary outcomes include self-reported respiratory and skin infections, and reported changes in hygiene practices, household water usage and water supply preference. River, tank and tap water from each village, and stored water from a subset of households, will be sampled to assess microbial and chemical quality. Ethics approval was obtained from the Monash University Human Research Ethics Committee in Australia and The Energy and Resources Institute Institutional Ethics Committee in India. The results of the trial will be presented at conferences, published in peer-reviewed journals and disseminated to relevant stakeholders. This study is funded by an Australian National Health and Medical Research Council (NHMRC) project grant. ACTRN12616001286437; pre

  18. An Italian Education: IEEE Pulse talks with Riccardo Pietrabissa, president of Italy's National Bioengineering Group, about Italian progress and challenges in biomedical engineering education.

    Science.gov (United States)

    Pietrabissa, Riccardo; Reynolds, Pamela

    2015-01-01

    From Leonardo da Vinci's designs for ball bearings to the incredible engineering wizardry behind the Ferrari, the inventive, inquisitive, and ingenious spirit of the engineer has always lived--and thrived--in Italy. From education to research to product development, Italy has always been regarded as an engineering leader. But does this apply to biomedical engineering (BME)? Despite many successes, questions loom, as they do at engineering schools worldwide. Concerns such as whether BME programs are providing students with enough focused, practical, hands-on training remain at the forefront, as does the question of whether graduates will be able to find jobs in industry after university studies are over. Here, IEEE Pulse explores these topics with Riccardo Pietrabissa, president of the Gruppo Nazionale di Bioingegneria (National Bioengineering Group) and a full professor in the Department of Chemistry, Materials, and Chemical Engineering at Politecnico di Milano.

  19. The Proximal Medial Sural Nerve Biopsy Model: A Standardised and Reproducible Baseline Clinical Model for the Translational Evaluation of Bioengineered Nerve Guides

    Directory of Open Access Journals (Sweden)

    Ahmet Bozkurt

    2014-01-01

    Full Text Available Autologous nerve transplantation (ANT is the clinical gold standard for the reconstruction of peripheral nerve defects. A large number of bioengineered nerve guides have been tested under laboratory conditions as an alternative to the ANT. The step from experimental studies to the implementation of the device in the clinical setting is often substantial and the outcome is unpredictable. This is mainly linked to the heterogeneity of clinical peripheral nerve injuries, which is very different from standardized animal studies. In search of a reproducible human model for the implantation of bioengineered nerve guides, we propose the reconstruction of sural nerve defects after routine nerve biopsy as a first or baseline study. Our concept uses the medial sural nerve of patients undergoing diagnostic nerve biopsy (≥2 cm. The biopsy-induced nerve gap was immediately reconstructed by implantation of the novel microstructured nerve guide, Neuromaix, as part of an ongoing first-in-human study. Here we present (i a detailed list of inclusion and exclusion criteria, (ii a detailed description of the surgical procedure, and (iii a follow-up concept with multimodal sensory evaluation techniques. The proximal medial sural nerve biopsy model can serve as a preliminarynature of the injuries or baseline nerve lesion model. In a subsequent step, newly developed nerve guides could be tested in more unpredictable and challenging clinical peripheral nerve lesions (e.g., following trauma which have reduced comparability due to the different nature of the injuries (e.g., site of injury and length of nerve gap.

  20. Radiation Protection

    International Nuclear Information System (INIS)

    Loos, M.

    2002-01-01

    Major achievements of SCK-CEN's Radiation Protection Department in 2001 are described. The main areas for R and D of the department are enviromnental remediation, emergency planning, radiation protection research, low-level radioactvity measurements, safeguards and physics measurements, decision strategy research and policy support and social sciences in nuclear research. Main achievements for 2001 in these areas are reported

  1. Sun protection

    Science.gov (United States)

    ... sun exposure. The start of summer is when UV rays can cause the most skin damage. Use sun protection, even on cloudy days. Clouds and haze don't protect you from the sun. Avoid surfaces that reflect light, such as water, sand, concrete, snow, and areas ...

  2. Radiation protection

    International Nuclear Information System (INIS)

    1989-01-01

    A NRPB leaflet in the 'At-a-Glance' series explains in a simple but scientifically accurate way what radiation is, the biological effects and the relative sensitivity of different parts of the human body. The leaflet then discusses radiation protection principles, radiation protection in the UK and finally the effectiveness of this radiation protection as judged by a breakdown of the total dose received by an average person in the UK, a heavy consumer of Cumbrian seafood, an average nuclear industry worker and an average person in Cornwall. (UK)

  3. Protected Areas

    Data.gov (United States)

    Kansas Data Access and Support Center — This dataset shows the boundaries of properties in Kansas in public or institutional ownership that contain ecological resources that merit some level of protection....

  4. Protective clothing

    International Nuclear Information System (INIS)

    Malet, J.C.; Regnier, J.

    1979-01-01

    The present operational and intervention suits are described. Research work is currently in progress to improve the performance of the existing suits and to develop more resistant protective clothing. (author)

  5. Radiation protection

    International Nuclear Information System (INIS)

    Ures Pantazi, M.

    1994-01-01

    This work define procedures and controls about ionizing radiations. Between some definitions it found the following topics: radiation dose, risk, biological effects, international radioprotection bodies, workers exposure, accidental exposure, emergencies and radiation protection

  6. Employment protection

    OpenAIRE

    Stefano Scarpetta

    2014-01-01

    Laws on hiring and firing are intended to protect workers from unfair behavior by employers, to counter imperfections in financial markets that limit workers’ ability to insure themselves against job loss, and to preserve firm-specific human capital. But by imposing costs on firms’ adaptation to changes in demand and technology, employment protection legislation may reduce not only job destruction but also job creation, hindering the efficient allocation of labor and productivity growth....

  7. Environmental protection

    International Nuclear Information System (INIS)

    Martinez, A.S.

    1987-01-01

    The question of environment protection related to the use of nuclear energy aiming to power generation, based on the harmonic concept of economic and industrial development, preserving the environment, is discussed. A brief study of environmental impacts for some energy sources, including nuclear energy, to present the systems of a nuclear power plant which aim at environmental protection, is done. (M.C.K.) [pt

  8. Radiation protection

    International Nuclear Information System (INIS)

    Koelzer, W.

    1976-01-01

    The lecture is divided into five sections. The introduction deals with the physical and radiological terms, quantities and units. Then the basic principles of radiological protection are discussed. In the third section attention is paid to the biological effects of ionizing radiation. The fourth section deals with the objectives of practical radiological protection. Finally the emergency measures are discussed to be taken in radiation accidents. (HP) [de

  9. Copyright protection

    OpenAIRE

    Plchotová, Gabriela

    2011-01-01

    The aim of this thesis is to offer a straightforward manual to anyone who authors their own original work or who utilises the original work of other creators. As such, it is necessary to briefly and clearly explain the historical development and essential terms of authorship as a concept and the origin of the need for copyright protection. Furthermore, this thesis includes chapters on copyright protection development specifically in the Czech Republic and the current definition of related law...

  10. Radiological protection

    International Nuclear Information System (INIS)

    Azorin N, J.; Azorin V, J. C.

    2010-01-01

    This work is directed to all those people related with the exercise of the radiological protection and has the purpose of providing them a base of knowledge in this discipline so that they can make decisions documented on technical and scientist factors for the protection of the personnel occupationally exposed, the people in general and the environment during the work with ionizing radiations. Before de lack of a text on this matter, this work seeks to cover the specific necessities of our country, providing a solid presentation of the radiological protection, included the bases of the radiations physics, the detection and radiation dosimetry, the radiobiology, the normative and operational procedures associates, the radioactive wastes, the emergencies and the transport of the radioactive material through the medical and industrial applications of the radiations, making emphasis in the relative particular aspects to the radiological protection in Mexico. The book have 16 chapters and with the purpose of supplementing the given information, are included at the end four appendixes: 1) the radioactive waste management in Mexico, 2-3) the Mexican official standards related with the radiological protection, 4) a terms glossary used in radiological protection. We hope this book will be of utility for those people that work in the investigation and the applications of the ionizing radiations. (Author)

  11. Is there redundancy in bioengineering for molluscan assemblages on the rocky shores of central Chile? ¿Existe redundancia en la bioingeniería de los ensambles de moluscos de las costas rocosas de Chile central?

    Directory of Open Access Journals (Sweden)

    BRENDAN P KELAHER

    2007-06-01

    Full Text Available Bioengineers modify habitats via their own physical structures and substantially increase local diversity in marine ecosystems. On rocky shores, there are large overlaps in the composition of communities associated with bioengineers that form complex mat-like habitats. We investigated the potential for redundancy in habitat provision by these types of habitats by comparing diverse molluscan assemblages associated with Perumytilus purpuratus mussel beds and algal turfs of Corallina officinalis var. chilenis, Gelidium chilense and Gastroclonium cylindricum. At three times between September 2003 and January 2004, we sampled the molluscan assemblages associated with each bioengineer at similar tidal heights on two rocky shores on the coast of central Chile. Of the 31 molluscan species identified, 30 were found in Corallina and 19-22 were identified from the other habitats. The pool of species found associated with each bioengineer overlapped greatly, demonstrating the potential for redundancy in habitat provision and little habitat-specificity. However, multivariate and univariate analyses showed all bioengineers except Gastroclonium contained a unique molluscan assemblage for at least one time of sampling because of variation in frequency of occurrence, richness and total abundance. Recent studies have highlighted many anthropogenic and natural processes that directly influence the diversity and composition of bioengineering species on rocky shores. We demonstrate that the loss of any particular bioengineer would not substantially alter the overall pool of molluscan species on the rocky shores of Chile. The loss of any bioengineer except Gastroclonium would, however, result in decreased local biodiversity because the molluscan assemblages in Perumytilus, Corallina and Gelidium, each contained a significantly different community structure for at least one time of samplingEn los ecosistemas marinos los organismos bioingenieros modifican habitats a

  12. Radiation protection

    International Nuclear Information System (INIS)

    Jain, Aman; Sharma, Shivam; Parasher, Abhishek

    2014-01-01

    Radiation dose measurement, field of radiobiology, is considered to be critical factor for optimizing radiation protection to the health care practitioners, patients and the public. This lead to equipment that has dose - area product meters permanently installed. In many countries and even institution, the range of equipment is vast and with the opportunity for radiation protection and dose recording varies considerably. Practitioners must move with the changed demands of radiation protection but in many cases without assistance of modern advancements in technology Keeping the three basic safety measures Time, Dose and Shielding we can say 'Optimum dose is safe dose' instead of 'No dose is safe dose'. The purpose enclosed within the title 'Radiation Protection'. The use of radiation is expanding widely everyday around the world and crossing boundaries of medical imaging, diagnostic and. The way to get the ''As low as reasonably achievable' is only achievable by using methodology of radiation protection and to bring the concern of general public and practitioners over the hazards of un-necessary radiation dose. Three basic principles of radiation protection are time, distance and shielding. By minimizing the exposure time increasing the distance and including the shielding we can reduce the optimum range of dose. The ability of shielding material to attenuate radiation is generally given as half value layer. This is the thickness of the material which will reduce the amount of radiation by 50%. Lab coat and gloves must be worn when handling radioactive material or when working in a labeled radiation work area. Safety glasses or other appropriate splash shields should be used when handling radioactive material. 1. Reached to low dose level to occupational workers, public as per prescribed dose limit. 2. By mean of ALARA principle we achieved the protection from radiation besides us using the radiation for our benefit

  13. Machine Protection

    International Nuclear Information System (INIS)

    Schmidt, R

    2014-01-01

    The protection of accelerator equipment is as old as accelerator technology and was for many years related to high-power equipment. Examples are the protection of powering equipment from overheating (magnets, power converters, high-current cables), of superconducting magnets from damage after a quench and of klystrons. The protection of equipment from beam accidents is more recent. It is related to the increasing beam power of high-power proton accelerators such as ISIS, SNS, ESS and the PSI cyclotron, to the emission of synchrotron light by electron–positron accelerators and FELs, and to the increase of energy stored in the beam (in particular for hadron colliders such as LHC). Designing a machine protection system requires an excellent understanding of accelerator physics and operation to anticipate possible failures that could lead to damage. Machine protection includes beam and equipment monitoring, a system to safely stop beam operation (e.g. dumping the beam or stopping the beam at low energy) and an interlock system providing the glue between these systems. The most recent accelerator, the LHC, will operate with about 3 × 10 14 protons per beam, corresponding to an energy stored in each beam of 360 MJ. This energy can cause massive damage to accelerator equipment in case of uncontrolled beam loss, and a single accident damaging vital parts of the accelerator could interrupt operation for years. This article provides an overview of the requirements for protection of accelerator equipment and introduces the various protection systems. Examples are mainly from LHC, SNS and ESS

  14. Machine Protection

    CERN Document Server

    Schmidt, R

    2014-01-01

    The protection of accelerator equipment is as old as accelerator technology and was for many years related to high-power equipment. Examples are the protection of powering equipment from overheating (magnets, power converters, high-current cables), of superconducting magnets from damage after a quench and of klystrons. The protection of equipment from beam accidents is more recent. It is related to the increasing beam power of high-power proton accelerators such as ISIS, SNS, ESS and the PSI cyclotron, to the emission of synchrotron light by electron–positron accelerators and FELs, and to the increase of energy stored in the beam (in particular for hadron colliders such as LHC). Designing a machine protection system requires an excellent understanding of accelerator physics and operation to anticipate possible failures that could lead to damage. Machine protection includes beam and equipment monitoring, a system to safely stop beam operation (e.g. dumping the beam or stopping the beam at low energy) and an ...

  15. Erosion Control and Recultivation Measures at a Headrace Channel of a Hydroelectric Power Plant using Different Combined Soil Bioengineering Techniques

    Science.gov (United States)

    Obriejetan, M.; Florineth, F.; Rauch, H. P.

    2012-04-01

    As a consequence of land use change resulting in an increased number of slope protection constructions and with respect to effects associated with climate change like extremes in temperatures and temperature variations or increased frequency of heavy precipitation, adaptation strategies for sustainable erosion protection systems are needed which meet ecological compatibility and economical requirements. Therefore a wide range of different technical solutions respectively geotextiles and geotextile-related products (blankets, nettings, grids etc.) are available on the market differing considerably in function, material, durability and pricing. Manufacturers usually provide product-specific information pertaining to application field, functional range or (technical) installation features whereas vegetational aspects are frequently neglected while vegetation can contribute substantially to increased near-surface erosion protection respectively slope stability. Though, the success of sustainable erosion control is directly dependent on several vegetational aspects. Adequate development of a functional vegetation layer in combination with geotextiles is closely associated to application aspects such as seeding technique, sowing date and intensity, seed-soil contact or maintenance measures as well as to qualitative aspects like seed quality, germination rates, area of origin, production method or certification. As a general guideline, erosion control within an initial phase is directly related to restoration techniques whereas vegetation specifics with regard to species richness or species composition play a key role in medium to long-term development and slope protection. In this context one of the fundamental objectives of our study is the identification and subsequently the determination of the main interaction processes between technical and biological components of combined slope protection systems. The influence of different geotextile characteristics on specific

  16. Developing control algorithms of a voluntary cough for an artificial bioengineered larynx using surface electromyography of chest muscles: A prospective cohort study.

    Science.gov (United States)

    Banus, M S; Birchall, M A; Graveston, J A

    2018-04-01

    This prospective cohort study investigates the prediction of a voluntary cough using surface electromyography (EMG) of intercostal and diaphragm muscles, to develop control algorithms for an EMG-controlled artificial larynx. The Ear Institute, London. Electromyography onset compared to voluntary cough exhalation onset and to 100 ms (to give the artificial larynx the time to close the bioengineered vocal cords) before voluntary cough exhalation onset, in twelve healthy participants. In the 189 EMG of intercostal muscle-detected voluntary coughs, 172 coughs (91% CI 70-112) were detected before onset of cough exhalation and 128 coughs (67.6% CI 33.7-101.7) 100 ms before onset of cough exhalation. In the 158 EMG of diaphragm muscle-detected voluntary coughs, 149 coughs (94.3% CI 76.3-112.3) were detected before onset of cough exhalation and 102 coughs (64.6% CI 26.6-102.6) 100 ms before onset of cough exhalation. More coughs were detected before onset of cough exhalation when combining EMG activity of intercostal and diaphragm muscles and comparing this to intercostal muscle activity alone (183 coughs [96.8% CI 83.8-109.8] vs 172 coughs, P = .0294). When comparing the mentioned combination to diaphragm muscle activity alone, the higher percentage of detected coughs before cough exhalation onset was not found to be significant (183 coughs vs 149 coughs, P = .295). In addition, more coughs were detected 100 ms before onset of cough exhalation with the mentioned combination of EMG activity and comparing this to intercostal muscles alone (149 coughs [78.8% CI 48.8-108.8] vs 128 coughs, P = .0198) and to diaphragm muscles alone (149 coughs vs 102 coughs, P = .0038). Most voluntary coughs can be predicted based on combined EMG signals of intercostal and diaphragm muscles, and therefore, these two muscle groups will be useful in controlling the bioengineered vocal cords within the artificial larynx during a voluntary cough. © 2017 John Wiley & Sons Ltd.

  17. Bioengineered Temporomandibular Joint Disk Implants: Study Protocol for a Two-Phase Exploratory Randomized Preclinical Pilot Trial in 18 Black Merino Sheep (TEMPOJIMS)

    Science.gov (United States)

    Monje, Florencio Gil; González-García, Raúl; Little, Christopher B; Mónico, Lisete; Pinho, Mário; Santos, Fábio Abade; Carrapiço, Belmira; Gonçalves, Sandra Cavaco; Morouço, Pedro; Alves, Nuno; Moura, Carla; Wang, Yadong; Jeffries, Eric; Gao, Jin; Sousa, Rita; Neto, Lia Lucas; Caldeira, Daniel; Salvado, Francisco

    2017-01-01

    Background Preclinical trials are essential to test efficacious options to substitute the temporomandibular joint (TMJ) disk. The contemporary absence of an ideal treatment for patients with severe TMJ disorders can be related to difficulties concerning the appropriate study design to conduct preclinical trials in the TMJ field. These difficulties can be associated with the use of heterogeneous animal models, the use of the contralateral TMJ as control, the absence of rigorous randomized controlled preclinical trials with blinded outcomes assessors, and difficulties involving multidisciplinary teams. Objective This study aims to develop a new, reproducible, and effective study design for preclinical research in the TMJ domain, obtaining rigorous data related to (1) identify the impact of bilateral discectomy in black Merino sheep, (2) identify the impact of bilateral discopexy in black Merino sheep, and (3) identify the impact of three different bioengineering TMJ discs in black Merino sheep. Methods A two-phase exploratory randomized controlled preclinical trial with blinded outcomes is proposed. In the first phase, nine sheep are randomized into three different surgical bilateral procedures: bilateral discectomy, bilateral discopexy, and sham surgery. In the second phase, nine sheep are randomized to bilaterally test three different TMJ bioengineering disk implants. The primary outcome is the histological gradation of TMJ. Secondary outcomes are imaging changes, absolute masticatory time, ruminant time per cycle, ruminant kinetics, ruminant area, and sheep weight. Results Previous preclinical studies in this field have used the contralateral unoperated side as a control, different animal models ranging from mice to a canine model, with nonrandomized, nonblinded and uncontrolled study designs and limited outcomes measures. The main goal of this exploratory preclinical protocol is to set a new standard for future preclinical trials in oromaxillofacial surgery

  18. Machine Protection

    International Nuclear Information System (INIS)

    Zerlauth, Markus; Schmidt, Rüdiger; Wenninger, Jörg

    2012-01-01

    The present architecture of the machine protection system is being recalled and the performance of the associated systems during the 2011 run will be briefly summarized. An analysis of the causes of beam dumps as well as an assessment of the dependability of the machine protection systems (MPS) itself is being presented. Emphasis will be given to events that risked exposing parts of the machine to damage. Further improvements and mitigations of potential holes in the protection systems will be evaluated along with their impact on the 2012 run. The role of rMPP during the various operational phases (commissioning, intensity ramp up, MDs...) will be discussed along with a proposal for the intensity ramp up for the start of beam operation in 2012

  19. Machine Protection

    CERN Document Server

    Zerlauth, Markus; Wenninger, Jörg

    2012-01-01

    The present architecture of the machine protection system is being recalled and the performance of the associated systems during the 2011 run will be briefly summarized. An analysis of the causes of beam dumps as well as an assessment of the dependability of the machine protection systems (MPS) itself is being presented. Emphasis will be given to events that risked exposing parts of the machine to damage. Further improvements and mitigations of potential holes in the protection systems will be evaluated along with their impact on the 2012 run. The role of rMPP during the various operational phases (commissioning, intensity ramp up, MDs...) will be discussed along with a proposal for the intensity ramp up for the start of beam operation in 2012.

  20. Machine Protection

    Energy Technology Data Exchange (ETDEWEB)

    Zerlauth, Markus; Schmidt, Rüdiger; Wenninger, Jörg [European Organization for Nuclear Research, Geneva (Switzerland)

    2012-07-01

    The present architecture of the machine protection system is being recalled and the performance of the associated systems during the 2011 run will be briefly summarized. An analysis of the causes of beam dumps as well as an assessment of the dependability of the machine protection systems (MPS) itself is being presented. Emphasis will be given to events that risked exposing parts of the machine to damage. Further improvements and mitigations of potential holes in the protection systems will be evaluated along with their impact on the 2012 run. The role of rMPP during the various operational phases (commissioning, intensity ramp up, MDs...) will be discussed along with a proposal for the intensity ramp up for the start of beam operation in 2012.

  1. Physical protection

    International Nuclear Information System (INIS)

    Myre, W.C.; DeMontmollin, J.M.

    1989-01-01

    Serious concern about physical protection of nuclear facilities began around 1972. R and D was initiated at Sandia National Laboratories which had developed techniques to protect weapons for many years. Special vehicles, convoy procedures, and a communications system previously developed for weapons shipments were improved and extended for shipments of other sensitive materials. Barriers, perimeter alarms, portal and internal control systems were developed, tested, and published in handbooks and presented at symposia. Training programs were initiated for U.S. and foreign personnel. Containment and surveillance techniques were developed for the IAEA. Presently emphasis is on computer security, active barriers, and techniques to prevent theft or sabotage by ''insiders''

  2. Negotiating Protection

    DEFF Research Database (Denmark)

    Bille, Mikkel

    efficacy. Some informants, for example, adopt an orthodox scriptural Islamic approach to protection and denounce certain material registers as un-Islamic and materialistic leftovers from an ignorant past, and rather prescribe Qur'anic remembrance. For other informants the very physicality of such contested...

  3. Protection Myopia

    DEFF Research Database (Denmark)

    Laursen, Keld; Salter, Ammon; Li, Cher

    from having an orientation towards legal appropriability, we conjecture that protection myopia may lead some firms to allocate too much attention to legal appropriability, in particular when the behavioral and structural contingencies are unfavorable. Examining a panel of three successive waves...

  4. Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair.

    Science.gov (United States)

    Wang, Ting-Yi; Bruggeman, Kiara A F; Sheean, Rebecca K; Turner, Bradley J; Nisbet, David R; Parish, Clare L

    2014-05-23

    Various engineering applications have been utilized to deliver molecules and compounds in both innate and biological settings. In the context of biological applications, the timely delivery of molecules can be critical for cellular and organ function. As such, previous studies have demonstrated the superiority of long-term protein delivery, by way of protein tethering onto bioengineered scaffolds, compared with conventional delivery of soluble protein in vitro and in vivo. Despite such benefits little knowledge exists regarding the stability, release kinetics, longevity, activation of intracellular pathway, and functionality of these proteins over time. By way of example, here we examined the stability, degradation and functionality of a protein, glial-derived neurotrophic factor (GDNF), which is known to influence neuronal survival, differentiation, and neurite morphogenesis. Enzyme-linked immunosorbent assays (ELISA) revealed that GDNF, covalently tethered onto polycaprolactone (PCL) electrospun nanofibrous scaffolds, remained present on the scaffold surface for 120 days, with no evidence of protein leaching or degradation. The tethered GDNF protein remained functional and capable of activating downstream signaling cascades, as revealed by its capacity to phosphorylate intracellular Erk in a neural cell line. Furthermore, immobilization of GDNF protein promoted cell survival and differentiation in culture at both 3 and 7 days, further validating prolonged functionality of the protein, well beyond the minutes to hours timeframe observed for soluble proteins under the same culture conditions. This study provides important evidence of the stability and functionality kinetics of tethered molecules. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  5. Protective articles

    International Nuclear Information System (INIS)

    Wardley, R.B.

    1983-01-01

    This patent specification describes an article affording protection against radiation, and especially against X-rays comprising at least one flexible layer of lead filled material in an envelope of, or sandwiched between two layers of a knitted, woven or non-woven fabric preferably of synthetic fibrous material, carrying on its outer surface a coating of flexible polyurethane. The outer fabric provides a resilient, extremely tough and cut resistant covering for the relatively soft lead filled material. (author)

  6. Eye Protection

    OpenAIRE

    Pashby, Tom

    1986-01-01

    Eye injuries frequently occur in the home, at work and at play. Many result in legally blind eyes, and most are preventable. Awareness of potential hazards is essential to preventing eye injuries, particularly in children. In addition, protective devices must be used appropriately. We have developed eye protectors that have proved effective in reducing both the overall incidence and the severity of sports eye injuries.

  7. A training syllabus for radiation protection in dental radiology

    International Nuclear Information System (INIS)

    Gallagher, A.; Dowling, A.; Renehan, J.; Clarke, D.; Malone, J. F.

    2008-01-01

    The EU Council Directive 97/43/EURATOM (MED) states that Member States shall ensure that adequate theoretical and practical training is provided for dental practitioners working with ionising radiation; this also includes the provision of continuing education and training programmes, post-qualification. The area of dental radiology is specifically mentioned in this legally binding document. The Dept. of Medical Physics and Bioengineering, St James's Hospital, Dublin, is particularly interested in the area of radiation protection training and routinely provides educational courses both at national and international levels. A recent review of their dental radiation protection course was undertaken in conjunction with a number of Principal Dental Surgeons within the Health Service Executive in Ireland. The revised course was delivered to over 200 dental staff members at two separate meetings during 2006. The response from attendees was very positive. It is proposed to extend this course to other dental professionals, working both in the Irish private and public health sectors in the future. (authors)

  8. A training syllabus for radiation protection in dental radiology.

    LENUS (Irish Health Repository)

    Gallagher, A

    2008-01-01

    The EU Council Directive 97\\/43\\/EURATOM (MED) states that Member States shall ensure that adequate theoretical and practical training is provided for dental practitioners working with ionising radiation; this also includes the provision of continuing education and training programmes, post-qualification. The area of dental radiology is specifically mentioned in this legally binding document. The Department of Medical Physics and Bioengineering, St James\\'s Hospital, Dublin, is particularly interested in the area of radiation protection training and routinely provides educational courses both at national and international levels. A recent review of their dental radiation protection course was undertaken in conjunction with a number of Principal Dental Surgeons within the Health Service Executive in Ireland. The revised course was delivered to over 200 dental staff members at two separate meetings during 2006. The response from attendees was very positive. It is proposed to extend this course to other dental professionals, working both in the Irish private and public health sectors in the future.

  9. [Cerebral protection].

    Science.gov (United States)

    Cattaneo, A D

    1993-09-01

    Cerebral protection means prevention of cerebral neuronal damage. Severe brain damage extinguishes the very "human" functions such as speech, consciousness, intellectual capacity, and emotional integrity. Many pathologic conditions may inflict injuries to the brain, therefore the protection and salvage of cerebral neuronal function must be the top priorities in the care of critically ill patients. Brain tissue has unusually high energy requirements, its stores of energy metabolites are small and, as a result, the brain is totally dependent on a continuous supply of substrates and oxygen, via the circulation. In complete global ischemia (cardiac arrest) reperfusion is characterized by an immediate reactive hyperemia followed within 20-30 min by a delayed hypoperfusion state. It has been postulated that the latter contributes to the ultimate neurologic outcome. In focal ischemia (stroke) the primary focus of necrosis is encircled by an area (ischemic penumbra) that is underperfused and contains neurotoxic substances such as free radicals, prostaglandins, calcium, and excitatory neurotransmitters. The variety of therapeutic effort that have addressed the question of protecting the brain reflects their limited success. 1) Barbiturates. After an initial enthusiastic endorsement by many clinicians and years of vigorous controversy, it can now be unequivocally stated that there is no place for barbiturate therapy following resuscitation from cardiac arrest. One presumed explanation for this negative statement is that cerebral metabolic suppression by barbiturates (and other anesthetics) is impossible in the absence of an active EEG. Conversely, in the event of incomplete ischemia EEG activity in usually present (albeit altered) and metabolic suppression and hence possibly protection can be induced with barbiturates. Indeed, most of the animal studies led to a number of recommendations for barbiturate therapy in man for incomplete ischemia. 2) Isoflurane. From a cerebral

  10. Mussel farming as a large-scale bioengineering tool: a numerical modelling case study in Rødsand lagoon, Denmark

    DEFF Research Database (Denmark)

    Forsberg, Pernille Louise; Ernstsen, Verner Brandbyge; Lumborg, Ulrik

    Rødsand lagoon in southeast Denmark is a non-tidal coastal lagoon, which is home to a wide range of marine flora and fauna and is protected under Natura 2000. An increase in turbidity within the lagoon may reduce the ecosystem health due to hindered light penetration. A threat to the lagoonal...... ecosystem is related to future increasing storm intensities (Olesen et al., 2014), which are presumed to increase the suspended sediment concentration (SSC) within the lagoon. Furthermore the planned construction of a tunnel between Germany and Denmark may propose a threat to the lagoon due to a possible...... spill of sediment, which could increase the longshore sediment influx to Rødsand lagoon. Mussels can reduce the SSC in marine environments (Schröder et al., 2014), why the implementation of a mussel farm has been considered as a management option. In the present study we developed a module to include...

  11. Concepts of radiation protection

    International Nuclear Information System (INIS)

    2013-01-01

    This seventh chapter presents the concepts and principles of safety and radiation protection, emergency situations; NORM and TENORM; radiation protection care; radiation protection plan; activities of the radiation protection service; practical rules of radiation protection and the radiation symbol

  12. Environmental protection

    International Nuclear Information System (INIS)

    Hull, A.P.

    1979-01-01

    Environmental Studies and Internal Dosimetry projects include: Environmental Protection; 1977 Environmental Monitoring Report; Sewage Sludge Disposal on the Sanitary Landfill; Radiological Analyses of Marshall Islands Environmental Samples, 1974 to 1976; External Radiation Survey and Dose Predictions for Rongelap, Utirik, Rongerik, Ailuk, and Wotje Atolls; Marshall Islands - Diet and Life Style Study; Dose Reassessment for Populations on Rongelap and Utirik Following Exposure to Fallout from BRAVO Incident (March 1, 1954); Whole Body Counting Results from 1974 to 1979 for Bikini Island Residents; Dietary Radioactivity Intake from Bioassay Data, a Model Applied to 137 Cs Intake by Bikini Island Residents; and External Exposure Measurements at Bikini Atoll

  13. Radiation protection

    International Nuclear Information System (INIS)

    Kamalaksh Shenoy, K.

    2013-01-01

    Three main pillars underpin the IAEA's mission: Safety and Security - The IAEA helps countries to upgrade their infrastructure for nuclear and radiation safety and security, and to prepare for and respond to emergencies. Work is keyed to international conventions, the development of international standards and the application of these standards. The aim is to protect people and the environment from the harmful effects of exposure to ionizing radiation. Science and Technology - The IAEA is the world's focal point for mobilizing peaceful applications of nuclear science and technology for critical needs in developing countries. The work contributes to alleviating poverty, combating disease and pollution of the environment and to other goals of sustainable development. Safeguards and Verification - The IAEA is the nuclear inspectorate, with more than four decades of verification experience. Inspectors work to verify that nuclear material and activities are not diverted towards military purposes. Quantities and Units: Dose equivalent is the product of absorbed dose of radiation and quality factor (Q). For absorbed dose in rads, dose equivalent is in rems. If absorbed dose is in gray, the dose equivalent is in sievert. Quality factor is defined without reference to any particular biological end point. Quality factors are recommended by committees such as the International Commission on Radiological Protection (ICRP) or the National Council on Radiation Protection and Measurements (NCRP), based on experimental RBE values but with some judgment exercised. Effective Dose Equivalent: It is the sum of the weighted dose equivalents for all irradiated tissues, in which the weighting factors represent the different risks of each tissue to mortality from cancer and hereditary effects. Committed dose equivalent: It is the integral over 50 years of dose equivalent following the intake of a radionuclide. Collective effective dose equivalent: It is a quantity for a population and is

  14. Protecting Antarctica

    Science.gov (United States)

    Carlowicz, Michael

    House Science Committee Chairman Robert Walker (R-Pa.) has introduced a bill into Congress to give the United States the legislative authority to implement the 1991 Environmental Protocol to the Antarctic Treaty. That protocol established rules and principles to shield the Antarctic environment from human spoilage—placing limits on the discharge of pollutants, protecting plant and animal life, and requiring environmental impact assessments before new activities and programs are launched. The protocol also forbids prospecting or developing of mineral resources except for scientific research.

  15. Food protection

    International Nuclear Information System (INIS)

    Gummer, Selwyn; Campbell-Savours, D.N.; Maxwell-Hyslop, R.

    1987-01-01

    The motion is to consolidate the Food Protection (Emergency Prohibitions) (England) (No 2) Order 1987 and seven subsequent amendment orders, into one order. The orders are those under which restrictions on the movement of sheep as a result of the Chernobyl accident are carried out. A debate lasting more than two hours followed and this is reported verbatim. Much of the early part of the debate concerns a telex message allegedly from the chief scientist of the radiochemical institute saying that meat contaminated by radiation after Chernobyl was sold in Britain. Both the source and the content of the telex were found not to be as alleged. In particular, the allegation that 10kg lambs had gone to market. The minister pointed out that these lambs were not big enough to be sold. The debate broadened into a more general discussion as to levels of contamination in sheep and the government diligence or otherwise in protecting the public. The motion was agreed to. (U.K.)

  16. Mycorrhizal inoculation as a tool for sustainable bio-engineering measures in steep alpine environments? - Results of a three year field experiment

    Science.gov (United States)

    Bast, Alexander; Wilcke, Wolfgang; Lüscher, Peter; Graf, Frank; Gärtner, Holger

    2013-04-01

    Global warming is anticipated to result in an increase of heavy precipitation events. In vegetation-free, steep Alpine areas intense rain fall events have distinct influences on erosional processes on slopes. These processes and (shallow) mass movements are directly linked with torrential rain falls, and for this lead to high erosion rates in those regions, resulting in an increased natural and socio-economic damage potential. For restoring and managing erosion-prone sites, bioengineering measures as a tool for hazard prevention gain more importance. Due to the rough environmental conditions, and hence, reduced germination capability and sprout vigour, it is difficult to establish a dense cover of pioneer vegetation. Thus, the question is what can be done to give planted saplings within bioengineering projects maximum support, to develop their above- and belowground structures to promote slope stabilization. Green-house and laboratory experiments have shown that mycorrhizal inoculum has a positive impact on plant development and soil structure, e.g. the formation of (stable) aggregates within several months. Based on these promising results, we intended to apply mycorrhizal inoculation in a field-experiment. In May 2010, we established experimental plots at an erosion-prone talus slope (inclination: ~40 - 45 °; elevation 1220 - 1360 m a.s.l.), located in the Eastern Swiss Alps. The slope, consisting of moraine and denudation-derived substrate, shows high geomorphic activity (e.g. debris flows, rill erosion). Two slope areas, 10m wide and 32m long, were stabilized with 1200 plants each. Additionally, mycorrhiza inoculum (INOQ Forst, 40 ml/plant) was added to one of the two areas. Within the stabilized areas, a mixture of eight saplings was planted per running meter in 15 rows. The assortment included four saplings of green alder and two of purple willow, as well as one tree (maple, birch, ash) and shrub species (e.g. guelder rose, honeysuckle). Finally, both areas

  17. Bio-Engineering tissue and V.A.C. therapy: A new method for the treatment of extensive necrotizing infection in the diabetic foot.

    Science.gov (United States)

    Armenio, Andrea; Cutrignelli, Daniela Anna; Nardulli, Maria Luisa; Maggio, Giulio; Memeo, Giuseppe; De Santis, Valerio; Giudice, Giuseppe; Ressa, Cosmo Maurizio

    2017-01-01

    The aim of the study is to compare the standard care for progressive necrotizing infection in diabetic foot with a treatment protocol based on the association between autologous fibroblast grafts and vacuum-assisted closure therapy (V.A.C.). A retrospective matched Case-Control study was carried out on 20 patients with diabetic foot infection, 10 treated with the standard care and 10 with our new protocol. Inclusion criteria were: acute diabetic foot necrosis (Wagner III and IV), ulcer size (30 to 80 cm2), tendon and bone exposure. Success in the treatment was evaluated as: percentage of healing at the 20th week, time of healing, deambulation, recurrence and major amputation rate. A 90% healing rate was observed after 20 weeks in the study group, compared to a 28.6% in the control group. The recurrence rate in the treated areas was 20% in the study group and 100% in the control group. None of the patients in either group required major amputations. We achieved very promising results by associating autologous fibroblasts grafts and V.A.C. therapy, in comparison with standard care. V.A.C. therapy seems to improve the growth rate of the fibroblasts, probably by sealing the wound and providing a moist environment following the fibroblast graft. The improved neoangiogenesis of the neo-dermis could explain the reduced recurrence rate of the study group. Despite the low number of patients involved and the retrospective nature of the analysis, this study showed a reliable, safe and cost-effective method of treating extensive infection in the diabetic foot. Bio-Engineered Tissue, Diabetic foot, Fibroblast graft, V.A.C.

  18. Radiation protection and environmental protection

    International Nuclear Information System (INIS)

    Xie Zi; Dong Liucan; Zhang Yongxing

    1994-01-01

    A collection of short papers is presented which review aspects of research in radiation and environmental protection carried out by the Chinese Institute of Atomic Energy in 1991. The topics covered are: the analysis of Po 210 in the gaseous effluent of coal-fired boilers; the determination of natural radionuclide levels in various industrial waste slags and management countermeasures; assessment of the collective radiation dose from natural sources for the Chinese population travelling by water; the preliminary environmental impact report for the multipurpose heavy water research reactor constructed by China for the Islamic Republic of Algeria. (UK)

  19. Protective clothing

    International Nuclear Information System (INIS)

    Winnett, G.F.

    1979-01-01

    A protective suit used for isolating its wearer from radioactively contaminated areas is described in three parts. The first part includes the covering for the wearer's head, arms and upper body and at the waist is releasably fitted around an opening into the contaminated area. The second part includes the legs of the suit and is releasably connectible to the first part of the suit to enclose the wearer who is then supplied with air through an umbilical pipe. A further part surrounds the second part and is releasably connectible to it, enclosing a space between the parts. This further part is also releasably connectible to the opening at the waist to prevent egress from the contaminated area. The releasable connections between the parts may be bayonet type fittings or may be rotating T-shaped projections which engage in T-shaped grooves. (author)

  20. Fire protection

    International Nuclear Information System (INIS)

    Janetzky, E.

    1980-01-01

    Safety and fire prevention measurements have to be treated like the activities developing, planning, construction and erection. Therefore it is necessary that these measurements have to be integrated into the activities mentioned above at an early stage in order to guarantee their effectiveness. With regard to fire accidents the statistics of the insurance companies concerned show that the damage caused increased in the last years mainly due to high concentration of material. Organization of fire prevention and fire fighting, reasons of fire break out, characteristics and behaviour of fire, smoke and fire detection, smoke and heat venting, fire extinguishers (portable and stationary), construction material in presence of fire, respiratory protection etc. will be discussed. (orig./RW)

  1. Protecting Democracy

    DEFF Research Database (Denmark)

    Galster, Kjeld

    2007-01-01

    of a democratic state as defence per se is to its government. Democratic governance rests on the mutual dependence and influence of leadership and led, and the practical function subsumes professional bureaucracies. Thus, defence debate is the exchange of views on matters important to national security amongst...... the democratically elected leadership, the public, and the defence bureaucracy’s professional experts. Defence debate is a decisive contributor to defence policy and it normally includes quantitative issues like the size of military forces and the proportion of the state’s resources devoted to the armed forces......ABSTRACT Galster, Kjeld Hald. Doctoral Student (History). Saxo Institute. May 2007. Protecting Democracy: Danish Defence Debate in Times of Change. Supervisor: Professor, Dr. Gunner Lind. Democratic debate on defence and democratic organisation of the forces are as central to the life...

  2. Protective Coatings

    Science.gov (United States)

    1980-01-01

    General Magnaplate Corporation's pharmaceutical machine is used in the industry for high speed pressing of pills and capsules. Machine is automatic system for molding glycerine suppositories. These machines are typical of many types of drug production and packaging equipment whose metal parts are treated with space spinoff coatings that promote general machine efficiency and contribute to compliance with stringent federal sanitation codes for pharmaceutical manufacture. Collectively known as "synergistic" coatings, these dry lubricants are bonded to a variety of metals to form an extremely hard slippery surface with long lasting self lubrication. The coatings offer multiple advantages; they cannot chip, peel or be rubbed off. They protect machine parts from corrosion and wear longer, lowering maintenance cost and reduce undesired heat caused by power-robbing friction.

  3. Offspring Protection

    Directory of Open Access Journals (Sweden)

    Eric T. Steiner

    2016-08-01

    Full Text Available Parental aggression, that is, offspring protection aggression, can be viewed as a type of parental investment. Most mammalian males do not exhibit parental investment and therefore exhibit little, if any, parental aggression. Men demonstrate parental investment, and are typically more physically aggressive than women, but parental physical aggression in humans has been largely unexplored. The current study examined potential sex differences in estimates of parental physical aggression involving hypothetical situations, while controlling for general physical aggression. A self-report measure was administered to 217 students from a western U.S. university (55 male nonparents, 50 female nonparents, 54 fathers, and 58 mothers. Male nonparents reported higher parental physical aggression than female nonparents, but there was no difference between mothers and fathers. The results are interpreted in light of ancestral effects of sexual selection and proximal effects of sex differences in testosterone, risk taking, and fear aversion.

  4. The role of the implementation angle of cuttings of Phyllanthus sellowianus as a reference for a soil protection measure against surface erosion

    Science.gov (United States)

    Rauch, H. P.; Sutili, F. J.; Aschbacher, M.; Müller, B.

    2009-04-01

    Cutting plantation is a very common method of soil bioengineering techniques. The potential of vegetative reproduction is used to install a vegetation cover on eroded slopes to prevent surface erosion. The development of above and below biomass from parts of the stock plant in a very short time and the fast and easy propagation are one of the most important advantages of this soil bioengineering type. Several handbooks (Schiechtl, 1992; Florineth, 2004 and Zeh, 2007) suggest potential plants for vegetative reproduction and describe the procedure of plantation in detail. It is recommended that the cuttings are not driven vertically into the ground. A flat implementation angle guarantees a more uniform rooting of the cutting part driven into the soil, however there are no systematically investigations of the impact of the implementation angle on the biomass performance and consequently on the performance as a surface erosion protection measure. This paper shows results from field investigations focusing on the problem of the impact of the implementation angle of cuttings. In sum 75 specimens of the species of Phyllanthus sellowianus. The plant species was recommended as a native potential soil bioengineering plant by Sutili (s. Sutili, 2006). The cuttings were planted with an average length of 50 cm and diameter of 2 cm. The implementation angle differences between 90 (vertical) 45 and 10 degree. Two months after plantation all plants were excavated and the relevant plant data sets were collected in order to analyse the biomass performance. The field investigations are part of an integrated research project of the University of Natural Resources and Applied Life Sciences, Vienna and the Federal University of Santa Maria, Rio Grande do Sul - Brazil.

  5. Pharmacological strategies for protection of extrahepatic islet transplantation.

    Science.gov (United States)

    Omori, K; Komatsu, H; Rawson, J; Mullen, Y

    2015-06-01

    The safety and effectiveness of islet transplantation has been proven through world-wide trials. However, acute and chronic islet loss has hindered the ultimate objective of becoming a widely used treatment option for type 1 diabetes. A large islet loss is attributed, in part, to the liver being a less-than-optimal site for transplantation. Over half of the transplanted islets are destroyed shortly after transplantation due to direct exposure to blood and non-specific inflammation. Successfully engrafted islets are continuously exposed to the liver micro-environment, a unique immune system, low oxygen tension, toxins and high glucose, which is toxic to islets, leading to premature islet dysfunction/death. Investigations have continued to search for alternate sites to transplant islets that provide a better environment for prolonged function and survival. This article gathers courses and conditions that lead to islet loss, from organ procurement through islet transplantation, with special emphasis on hypoxia, oxidative stress, and antigen non-specific inflammation, and reviews strategies using pharmacological agents that have shown effectiveness in protecting islets, including a new treatment approach utilizing siRNA. Pharmacological agents that support islet survival and promote β-cell proliferation are also included. Treatment of donor pancreata and/or islets with these agents should increase the effectiveness of islets transplanted into extrahepatic sites. Furthermore, the development of methods designed to release these agents over an extended period, will further increase their efficacy. This requires the combined efforts of both islet transplant biologists and bioengineers.

  6. The REMEDEE-OCT Study: An Evaluation of the Bioengineered COMBO Dual-Therapy CD34 Antibody-Covered Sirolimus-Eluting Coronary Stent Compared With a Cobalt-Chromium Everolimus-Eluting Stent in Patients With Acute Coronary Syndromes: Insights From Optical Coherence Tomography Imaging Analysis

    NARCIS (Netherlands)

    Jaguszewski, Milosz; Aloysius, Romila; Wang, Wei; Bezerra, Hiram G.; Hill, Jonathan; de Winter, Robbert J.; Karjalainen, Pasi P.; Verheye, Stefan; Wijns, William; Lüscher, Thomas F.; Joner, Michael; Costa, Marco; Landmesser, Ulf

    2017-01-01

    The aim of the present study was to evaluate vascular healing of the bioengineered COMBO Dual Therapy Stent compared with a cobalt-chromium (CoCr) everolimus-eluting stent (EES) as assessed by optical coherence tomography in patients with acute coronary syndromes. CD34+ cells promote endothelial

  7. REMOTE SENSING FOR BIOENGINEERED CROPS

    Science.gov (United States)

    Increasing interest in the responsible management of technology in the industrial and agricultural sectors of the economy has been met through the development of broadly applicable tools to assess the "sustainability" of new technologies. An arena ripe for application of such ana...

  8. Bio-engineering - A Perspective

    African Journals Online (AJOL)

    of modern life. The truism that medicine is ... balance between clinical medicine and laboratory ... this type of work accept its interdisciplinary nature, and those institutions ... representing strain for given stress, with long axes gene- rally running ...

  9. Tissue bioengineering and artificial organs.

    Science.gov (United States)

    Llames, Sara; García, Eva; Otero Hernández, Jesús; Meana, Alvaro

    2012-01-01

    The scarcity of organs and tissues for transplant and the need of immunosuppressive drugs to avoid rejection constitute two reasons that justify organ and tissue production in the laboratory. Tissue engineering based tissues (TE) could allow to regenerate the whole organ from a fragment or even to produce several organs from an organ donor for grafting purposes. TE is based in: (1) the ex vivo expansion of cells, (2) the seeding of these expanded cells in tridimensional structures that mimic physiological conditions and, (3) grafting the prototype. In order to graft big structures it is necessary that the organ or tissue produced "ex vivo" bears a vascular tree to ensure the nutrition of its deep layers. At present, no technology has been developed to provide this vascular tree to TE derived products. Thus, these tissues must be thin enough to acquire nutrients during the first days by diffusion from surrounding tissues. This fact constitutes nowadays the greatest limitation of technologies for organ development in the laboratory.In this chapter, all these problems and their possible solutions are commented. Also, the present status of TE techniques in the regeneration of different organ systems is reviewed.

  10. Skin Bioengineering: Noninvasive Transdermal Monitoring

    Science.gov (United States)

    2005-01-01

    chloroquine , promazine, tetracaine and metoclopramide) were administered iontophoretically (figure 6). Then, the anodal extraction of PGE2 from the site of... analogue of human colour this apparent thermo-optical response of the perception. Unlike the classical NIR approach skin can also provide an

  11. Radiation protection principles

    International Nuclear Information System (INIS)

    Ismail Bahari

    2007-01-01

    The presentation outlines the aspects of radiation protection principles. It discussed the following subjects; radiation hazards and risk, the objectives of radiation protection, three principles of the system - justification of practice, optimization of protection and safety, dose limit

  12. [Light protection: principles of UV protection].

    Science.gov (United States)

    Stege, H; Mang, R

    2006-05-01

    UV radiation is responsible for the induction of epithelial and melanocytic skin cancer, photoaging, and photodermatoses. UV protection is necessary to prevent damage caused by non-physiologic exposure. UV protection includes not only reduction of sun exposure but also use of sun protective filters, UV protective clothes, DNA repair enzymes, and antioxidant supplementation. Consumers are uncertain about the possibilities and limitations of commercial sun protection measures. Dermatologists must explain protective measures to the general public which continues to believe that UV-tanned skin is healthy. The sunscreen market is a highly competitive but lucrative market. The range of products with different designations and promises makes difficult for both consumers and dermatologists to determine what is sensible UV protection.

  13. Bioengineering applied to erosion and stability control in the North Apennines (Emilia-Romagna Region, Italy): a check about critical aspects of the works.

    Science.gov (United States)

    Selli, Lavinia; Cavazza, Claudio; Pavanelli, Donatella

    2013-04-01

    Because of its geological structure, in the Emilia-Romagna Region over 32,000 landslides have been identified. Several works have been made in order to control mass movement's dynamics and to secure of Reno and Lamone Mountain Basin Rivers, the road network and near by villages and towns. Most of the control works dealt with bioengineering practices: palisades piles, geotextiles, seedings, surface flow control works, dikes within main drainage ditches. In order to check about critical aspects related to the use of these techniques in the Apennines, a survey in this basins was designed with specific interest in the several kinds of works realised, in which plant species were mostly used and in the factors that affected the success or failure of the works. Territory encompasses steep slopes covered with woods to low reliefs covered with grasslands. It is characterized by prevailing clays, inducing instability, and arenaceous lithology with impermeable soils; drainage density is quite high and hillsides suffer extensive and severe erosion and slope stability problems. Chestnut woods mainly represent land use at higher altitudes, while coppice, pastures and crops are present on milder hillsides. The remaining part of the basin is covered by vineyards, orchards, ponds and urban areas, which are basically located in the valley floor. Precipitation events mainly consist of rainfall ranging between 950-1015 mm per year; few snowfalls occur during winter and a long dry season lasts from June until September. We have analyzed 187 works designed mainly for the consolidation of slope instabilities through a widespread enhancement of the vegetation cover. The surveyed works are classified as a function of their building features: it can be seen that cribwalls and palisades are by far the most common types, being the 24% and the 34% respectively of the works. As far as the most adopted plant species, they were silver willow (Salix alba), Spanish Broom (Spartium Junceum) and

  14. Laser Protection TIL

    Data.gov (United States)

    Federal Laboratory Consortium — The Laser Protection TIL conducts research and analysis of laser protection materials along with integration schemes. The lab's objectives are to limit energy coming...

  15. Vegetated Riprap Installation Techniques for Steambank Protection, Fish and Wildlife Habitat Creation

    Science.gov (United States)

    Raymond, Pierre

    2014-05-01

    Vegetated riprap is a cost effective alternative to conventional riprap erosion protection. Terra Erosion Control has experimented with the vegetation of riprap over the past ten years. As a result we have adapted a technique that can successfully establish vegetation during the installation of riprap structures. This presentation will demonstrate innovative ways of installing vegetated riprap for the protection of access roads on industrial sites and urban infrastructure such as storm water outfalls, bridge approaches and pedestrian pathways within public areas. This vegetation will provide additional bank protection, soften the rock appearance and enhance fish, wildlife and urban habitat along the shoreline. Vegetated riprap incorporates a combination of rock and native vegetation in the form of live cuttings. These are planted in conjunction with the placement of rock used to armour the banks of watercourses. Establishment of native vegetation will improve fish habitat by creating shade, cover and an input of small organic debris to stream banks. In most cases it will negate the need for the regulator (Canadian Department of Fisheries and Oceans) to require habitat alteration compensation. It will also provide added bank protection through the development of root mass. Adding vegetation to riprap provides a softer, more natural appearance to the installed rocks. This presentation will detail the processes involved in the installation of vegetated riprap such as the harvesting and soaking of live material, site preparation of the stream bank, placement of riprap in conjunction with live material and the use of burlap/coir fabric and soil amendments. It will also discuss the innovative method of using wooden boards to protect live cuttings during construction and to direct precipitation and/or irrigation water to the root zone during the establishment phase of the vegetation. These boards will eventually biodegrade within the rock. This approach was applied over

  16. Whistland: An Augmented Reality Crowd-Mapping System for Civil Protection and Emergency Management

    Directory of Open Access Journals (Sweden)

    Gioele Luchetti

    2017-02-01

    Full Text Available The prevention and correct management of natural disaster event sequences play a key role in saving human lives. The availability of embedded and mobile smart computing systems opens new roads for the management of land and infrastructures by civil protection operators. To date, research has explored the use of social networks for the management of disasters connected to meteorological/hydrogeological events or earthquakes, but without emphasis on the importance of an integrated system. The main feature of the Whistland system proposed in this paper is to make synergistic use of augmented reality (AR, crowd-mapping (CM, social networks, the Internet of Things (IoT and wireless sensor networks (WSN by exploiting technologies and frameworks of Web 2.0 and GIS 2.0 to make informed decisions about the chain of events. The Whistland system is composed of a geo-server, a mobile application with AR and an analytics dashboard. The geo-server acts as the hub of the sensor and social networks. The abstracted concept in this sense is the transformation of the user domain into “intelligent sensors” for the whole scope of crisis management. The social network integration is made through an efficient pointer-like mechanism that keeps the storage requirement low through a mobile application based on an augmented reality engine and provides qualitative information that sensors are unable to capture. Real-time analyses, geo-searches and the capability to examine event histories with an augmented reality engine all help the stakeholders to understand better the state of the resources under observation/monitoring. The system has been extensively tested in the programmed maintenance of river basins, where it is necessary to log maintenance activities in order to keep the riverbank clean: a significant use-case in many countries affected by hydro-geological instability.

  17. Environmental protection economically viable

    International Nuclear Information System (INIS)

    Dartsch, B.; Hildebrandt, T.

    1994-01-01

    The Environmental Technology Forum for Industry and Research (Utech) was held for the fifth time this year at the International Congress Centre in Berlin. The main themes of this year's Utech were additive environmental protection, production-integrated environmental protection, management and economic aspects of environmental protection, research and development in environmental protection as well as environmental information legislation. (orig.) [de

  18. Nature and landscape protection

    International Nuclear Information System (INIS)

    Klinda, J.; Lieskovska, Z.

    1998-01-01

    In accordance with National Council of the Slovak Republic Act N. 287/1994 Coll. on Nature and Landscape Protection, the system of complex nature landscape protection has been designed based on five levels of protection. Categories of protected areas as well as cultural monuments in the Slovak Republic are reviewed.Slovak contribution to the world heritage is included

  19. Operational radiation protection and radiation protection training

    International Nuclear Information System (INIS)

    Kraus, W.

    1989-01-01

    The radiation protection system in the German Democratic Republic (GDR) is reviewed. The competent authority (the SAAS) and its systems of licensing and supervision are described. Discussion covers the role of the Radiation Protection Officer, the types of radiation monitoring, medical surveillance programs and the classification of workers and work areas. Unusual occurrences in the GDR, 1963-1976, are presented and the occupational radiation protection problems at some specific types of workplaces are discussed. The GDR's system of training in radiation protection and nuclear safety is described. 5 figs., 18 tabs

  20. Focus radiation protection

    International Nuclear Information System (INIS)

    Ebermann, Lutz

    2016-01-01

    The publication of the Bundesamt fuer Strahlenschutz on radiation protection covers the following issues: (i) exposure from natural sources: health hazard due to radon, radiation protection in residential homes, radon in Germany, natural raw materials in industrial processes; (ii) clearance of radioactive wastes: clearance in the frame of nuclear power plant dismantling, the situation in Germany and Europe; (iii) emergency management: principles of radiation protection, fictive sequence of accident events; (iiii) other actual radiation protection topics: more limits - more protection? radiation protection in medicine, occupational radiation protection.

  1. Radiation. Protection. Health. Proceedings

    International Nuclear Information System (INIS)

    Hajek, Michael; Maringer, Franz Josef; Steurer, Andreas; Schwaiger, Martina; Timal, Guenter

    2015-01-01

    The topics of the meeting are the diagnostic and therapeutic application of ionizing radiations, the application of radiation in research, industry and engineering and radiation protection. The volume includes the following chapters: Radiation protection and society, radiation protection infrastructure, population and environment, metrology and measuring techniques, 1. Workshop on population and environment, NORM and radon, 2. Update: dose - extent of damage - limiting value definition, radiation protection for personnel (except medicine), radiation protection in medicine.

  2. Loft fire protection

    International Nuclear Information System (INIS)

    White, E.R.; Jensen, J.D.

    1980-01-01

    Quantified criteria that was developed and applied to provide in-depth fire protection for the Loss of Fluid Test (LOFT) Facility are presented. The presentation describes the evolution process that elevated the facility's fire protection from minimal to that required for a highly protected risk or improved risk. Explored are some infrequently used fire protection measures that are poorly understood outside the fire protection profession

  3. Aircraft Fire Protection Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Navy Aircraft Protection Laboratory provides complete test support for all Navy air vehicle fire protection systems.The facility allows for the simulation of a...

  4. Noise and Hearing Protection

    Science.gov (United States)

    ... ENTCareers Marketplace Find an ENT Doctor Near You Noise and Hearing Protection Noise and Hearing Protection Patient ... it is. How can I tell if a noise is dangerous? People differ in their sensitivity to ...

  5. Radiation protection standards

    International Nuclear Information System (INIS)

    Koelzer, W.

    1980-01-01

    The present paper deals with: Objectives and basic concepts of radiation protection, basic radiobiological considerations, the ICRP system of dose limitation and with operational radiation protection (limits, reference levels, occupational exposure). (RW)

  6. Cathodic protection -- Rectifier 46

    International Nuclear Information System (INIS)

    Lane, W.M.

    1995-01-01

    This Acceptance Test Procedure (ATP) has been prepared to demonstrate that the cathodic protection system functions as required by project criteria. The cathodic protection system is for the tank farms on the Hanford Reservation. The tank farms store radioactive waste

  7. Healthy Watersheds Protection

    Science.gov (United States)

    ... for restoring areas with degraded water quality, as well as protecting healthy waters from emerging problems before expensive damages occur. ... exclusively on restoring impaired waters, EPA created the Healthy ... more emphasis to proactively protecting high quality waters, following the ...

  8. Cathodic protection -- Rectifier 47

    International Nuclear Information System (INIS)

    Lane, W.M.

    1995-01-01

    This Acceptance Test Procedure (ATP) has been prepared to demonstrate that the cathodic protection system functions as required by project criteria. The cathodic protection system is for the tank farms at the Hanford Reservation. The tank farms store radioactive waste

  9. Endangered Species Protection Bulletins

    Science.gov (United States)

    Endangered Species Protection Bulletins set forth geographically specific pesticide use limitations for the protection of threatened and endangered (listed) species and their designated critical habitat. Find out how to get and use Bulletins.

  10. Information protection playbook

    CERN Document Server

    Kane, Greg

    2013-01-01

    The primary goal of the Information Protection Playbook is to serve as a comprehensive resource for information protection (IP) professionals who must provide adequate information security at a reasonable cost. It emphasizes a holistic view of IP: one that protects the applications, systems, and networks that deliver business information from failures of confidentiality, integrity, availability, trust and accountability, and privacy. Using the guidelines provided in the Information Protection Playbook, security and information technology (IT) managers will learn how to

  11. Machine protection systems

    CERN Document Server

    Macpherson, A L

    2010-01-01

    A summary of the Machine Protection System of the LHC is given, with particular attention given to the outstanding issues to be addressed, rather than the successes of the machine protection system from the 2009 run. In particular, the issues of Safe Machine Parameter system, collimation and beam cleaning, the beam dump system and abort gap cleaning, injection and dump protection, and the overall machine protection program for the upcoming run are summarised.

  12. Environmental Protection Agency, Protecting Children's Environmental Health

    Science.gov (United States)

    ... and Research Centers Contact Us Share Protecting Children's Environmental Health Children are often more vulnerable to pollutants ... during development. Learn more about children's health, the environment, and what you can do. Basic Information Children ...

  13. Protective Behavior in Games

    OpenAIRE

    Fiestras-Janeiro, G.; Borm, P.E.M.; van Megen, F.J.C.

    1996-01-01

    This paper introduces the notion of protective equilibrium in the context of fin ite games in strategic form.It shows that for matrix games the set of protective equilibria equals the set of proper equilibria.Moreover, in the context of bima trix games, the notion of protective behaviour is used as a refinement tool.

  14. Protective Behavior in Games

    NARCIS (Netherlands)

    Fiestras-Janeiro, G.; Borm, P.E.M.; van Megen, F.J.C.

    1996-01-01

    This paper introduces the notion of protective equilibrium in the context of fin ite games in strategic form.It shows that for matrix games the set of protective equilibria equals the set of proper equilibria.Moreover, in the context of bima trix games, the notion of protective behaviour is used as

  15. Radiation protection seminar

    International Nuclear Information System (INIS)

    2012-01-01

    The Radiation Protection Seminar, was organized by the Argentina Association of Biology and Nuclear Medicine, and Bacon Laboratory, the 20 june 2012, in the Buenos Aires city of Argentina. In this event were presented some papers on the following topics: methods of decontamination, radiation protection of patients; concepts of radiation protection and dosimetry.

  16. Ozone Layer Protection

    Science.gov (United States)

    ... and Research Centers Contact Us Share Ozone Layer Protection The stratospheric ozone layer is Earth’s “sunscreen” – protecting ... GreenChill Partnership Responsible Appliance Disposal (RAD) Program Ozone Protection vs. Ozone Pollution This website addresses stratospheric ozone ...

  17. Designing physical protection technology for insider protection

    International Nuclear Information System (INIS)

    Trujillo, A.A.; Waddoups, I.G.

    1986-01-01

    Since its inception, the nuclear industry has been engaged in providing protection against an insider threat. Although insider protection activities have been fairly successful in the past, present societal conditions require increased protection to further minimize the existence of an insider or the consequences of an insider-perpetrated incident. Integration of insider protection techniques into existing administrative and operational procedures has resulted in economic and operational impacts. Future increases in insider protection may result in even greater impacts, so we must proceed wisely as new approaches are developed. Increased emphasis on background investigations, security clearances, human reliability programs, security awareness activities, and the development of technology to address the insider threat are evidence of continuing concern in this area. Experience ranging from operational test and evaluation of developmental equipment to conceptual designs for new facilities has led to the development of general principles and conclusions for mitigating the insider threat while minimizing adverse impacts on site operations. Important principles include real-time monitoring of personnel and material and requiring that the physical protection and material control and accounting systems to be much more coordinated and integrated than in the past

  18. Numerical distance protection

    CERN Document Server

    Ziegler, Gerhard

    2011-01-01

    Distance protection provides the basis for network protection in transmission systems and meshed distribution systems. This book covers the fundamentals of distance protection and the special features of numerical technology. The emphasis is placed on the application of numerical distance relays in distribution and transmission systems.This book is aimed at students and engineers who wish to familiarise themselves with the subject of power system protection, as well as the experienced user, entering the area of numerical distance protection. Furthermore it serves as a reference guide for s

  19. Radiation protection in Sudan

    International Nuclear Information System (INIS)

    Elamin, O.I.; Hajmusa, E.A.; Shaddad, I.A.

    2001-01-01

    The regulatory framework as established by the Sudan Atomic Energy Commission (SAEC) Act, promulgated in 1996, is described in the report. Three levels of responsibility in meeting radiation protection requirements are established: the Board, the Radiation Protection Technical Committee as the competent authority in the field of radiation protection, and the SAEC Department of Radiation Protection and Environmental Monitoring as the implementing technical body. The report also refers to environmental activities, patient doses in diagnostic radiology, the management of disused sources, emergency preparedness and orphan sources, and the national training activities in the radiation protection field. (author)

  20. Fire Protection Program Manual

    Energy Technology Data Exchange (ETDEWEB)

    Sharry, J A

    2012-05-18

    This manual documents the Lawrence Livermore National Laboratory (LLNL) Fire Protection Program. Department of Energy (DOE) Orders 420.1B, Facility Safety, requires LLNL to have a comprehensive and effective fire protection program that protects LLNL personnel and property, the public and the environment. The manual provides LLNL and its facilities with general information and guidance for meeting DOE 420.1B requirements. The recommended readers for this manual are: fire protection officers, fire protection engineers, fire fighters, facility managers, directorage assurance managers, facility coordinators, and ES and H team members.

  1. Numerical differential protection

    CERN Document Server

    Ziegler, Gerhard

    2012-01-01

    Differential protection is a fast and selective method of protection against short-circuits. It is applied in many variants for electrical machines, trans?formers, busbars, and electric lines.Initially this book covers the theory and fundamentals of analog and numerical differential protection. Current transformers are treated in detail including transient behaviour, impact on protection performance, and practical dimensioning. An extended chapter is dedicated to signal transmission for line protection, in particular, modern digital communication and GPS timing.The emphasis is then pla

  2. Personal protective equipment

    International Nuclear Information System (INIS)

    2004-01-01

    This Practical Radiation Technical Manual is one of a series that has been designed to provide guidance on radiological protection for employers, radiation protection officers, managers and other technically competent persons who have responsibility for ensuring the safety of employees working with ionizing radiation. The Manual may be used with the appropriate IAEA Practical Radiation Safety Manuals to provide training, instruction and information for all employees engaged in work with ionizing radiation. Personal protective equipment (PPE) includes clothing or other special equipment that is issued to individual workers to provide protection against actual or potential exposure to ionizing radiations. It is used to protect each worker against the prevailing risk of external or internal exposure in circumstances in which it is not reasonably practicable to provide complete protection by means of engineering controls or administrative methods. Adequate personal protection depends on PPE being correctly selected, fitted and maintained. Appropriate training for the users and arrangements to monitor usage are also necessary to ensure that PPE provides the intended degree of protection effectively. This Manual explains the principal types of PPE, including protective clothing and respiratory protective equipment (RPE). Examples of working procedures are also described to indicate how PPE should be used within a safe system of work. The Manual will be of most benefit if it forms part of a more comprehensive training programme or is supplemented by the advice of a qualified expert in radiation protection. Some of the RPE described in this Manual should be used under the guidance of a qualified expert

  3. Adaptive protection scheme

    Directory of Open Access Journals (Sweden)

    R. Sitharthan

    2016-09-01

    Full Text Available This paper aims at modelling an electronically coupled distributed energy resource with an adaptive protection scheme. The electronically coupled distributed energy resource is a microgrid framework formed by coupling the renewable energy source electronically. Further, the proposed adaptive protection scheme provides a suitable protection to the microgrid for various fault conditions irrespective of the operating mode of the microgrid: namely, grid connected mode and islanded mode. The outstanding aspect of the developed adaptive protection scheme is that it monitors the microgrid and instantly updates relay fault current according to the variations that occur in the system. The proposed adaptive protection scheme also employs auto reclosures, through which the proposed adaptive protection scheme recovers faster from the fault and thereby increases the consistency of the microgrid. The effectiveness of the proposed adaptive protection is studied through the time domain simulations carried out in the PSCAD⧹EMTDC software environment.

  4. Theory of vibration protection

    CERN Document Server

    Karnovsky, Igor A

    2016-01-01

    This text is an advancement of the theory of vibration protection of mechanical systems with lumped and distributed parameters. The book offers various concepts and methods of solving vibration protection problems, discusses the advantages and disadvantages of different methods, and the fields of their effective applications. Fundamental approaches of vibration protection, which are considered in this book, are the passive, parametric and optimal active vibration protection. The passive vibration protection is based on vibration isolation, vibration damping and dynamic absorbers. Parametric vibration protection theory is based on the Shchipanov-Luzin invariance principle. Optimal active vibration protection theory is based on the Pontryagin principle and the Krein moment method. The book also contains special topics such as suppression of vibrations at the source of their occurrence and the harmful influence of vibrations on humans. Numerous examples, which illustrate the theoretical ideas of each chapter, ar...

  5. Resilience from coastal protection.

    Science.gov (United States)

    Ewing, Lesley C

    2015-10-28

    Coastal areas are important residential, commercial and industrial areas; but coastal hazards can pose significant threats to these areas. Shoreline/coastal protection elements, both built structures such as breakwaters, seawalls and revetments, as well as natural features such as beaches, reefs and wetlands, are regular features of a coastal community and are important for community safety and development. These protection structures provide a range of resilience to coastal communities. During and after disasters, they help to minimize damages and support recovery; during non-disaster times, the values from shoreline elements shift from the narrow focus on protection. Most coastal communities have limited land and resources and few can dedicate scarce resources solely for protection. Values from shore protection can and should expand to include environmental, economic and social/cultural values. This paper discusses the key aspects of shoreline protection that influence effective community resilience and protection from disasters. This paper also presents ways that the economic, environmental and social/cultural values of shore protection can be evaluated and quantified. It presents the Coastal Community Hazard Protection Resilience (CCHPR) Index for evaluating the resilience capacity to coastal communities from various protection schemes and demonstrates the use of this Index for an urban beach in San Francisco, CA, USA. © 2015 The Author(s).

  6. Training in radiation protection

    International Nuclear Information System (INIS)

    Schreiber, F.

    1998-01-01

    Persons who are exposed to ionizing radiation at their workplace have to be trained in radiation protection. According to the Radiation Protection Ordinance the person with responsibility in radiation protection has to guarantee that the training is performed twice a year. Our training material was created especially for the persons defined in the Radiation Protection Ordinance and the X-ray Ordinance. It enables persons who teach (generally the radiation protection officer) to perform the training without tedious study and preparation of the documents. Our material is not just another textbook for radiation protection but rather a folder with colour transparencies and explanatory texts which make a difference in volume and price in comparison to other existing materials. (orig.) [de

  7. Protecting plutonium: physical safeguards

    International Nuclear Information System (INIS)

    Ney, J.F.

    1975-10-01

    In the development of physical protection systems, objectives for improving overall performance include the following: (1) Increase the time required for the malefactor to achieve his goal; (2) decrease the time required for detection of malevolent activities; (3) reduce the time for adequate response force arrival; (4) increase the capability to neutralize the malefactor; (5) reduce the total protection system costs, while increasing the level of protection; (6) improve acceptance levels (social, environmental, legal, and institutional); and (7) increase nuclear fuel cycle safety. Fortunately, there is sufficient lead time and technical base to explore and develop new protection system concepts so that a completely integrated and tested protection system capable of providing unprecedented levels of security can be available when needed. Although it will be impossible to completely eliminate all risks, it should be both possible and economically feasible to install protection systems which will deter or prevent a malefactor from using the nuclear fuel cycle to disrupt society

  8. Emergency Protection from Aerosols

    Energy Technology Data Exchange (ETDEWEB)

    Cristy, G.A.

    2001-11-13

    Expedient methods were developed that could be used by an average person, using only materials readily available, to protect himself and his family from injury by toxic (e.g., radioactive) aerosols. The most effective means of protection was the use of a household vacuum cleaner to maintain a small positive pressure on a closed house during passage of the aerosol cloud. Protection factors of 800 and above were achieved.

  9. Emergency protection from aerosols

    International Nuclear Information System (INIS)

    Cristy, G.A.; Chester, C.V.

    1981-07-01

    Expedient methods were developed that could be used by an average person, using only materials readily available, to protect himself and his family from injury by toxic (e.g., radioactive) aerosols. The most effective means of protection was the use of a household vacuum cleaner to maintain a small positive pressure on a closed house during passage of the aerosol cloud. Protection factors of 800 and above were achieved

  10. Protecting genetic privacy.

    Science.gov (United States)

    Roche, P A; Annas, G J

    2001-05-01

    This article outlines the arguments for and against new rules to protect genetic privacy. We explain why genetic information is different to other sensitive medical information, why researchers and biotechnology companies have opposed new rules to protect genetic privacy (and favour anti-discrimination laws instead), and discuss what can be done to protect privacy in relation to genetic-sequence information and to DNA samples themselves.

  11. Radiological protection act, 1991

    International Nuclear Information System (INIS)

    1991-01-01

    This Act provides for the establishment of the Radiological Protection Institute of Ireland and dissolves An Bord Fuinnimh Nuicleigh (the Board), transferring its assets and liabilities to the Institute. It sets out a range of radiation protection measures to be taken by various Ministers in the event of a radiological emergency and gives effect at national level to the Assistance Convention, the Early Notification Convention and the Physical Protection Convention. The Institute is the competent Irish authority for the three Conventions. (NEA) [fr

  12. Protection using telecommunications

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    Deregulation in both the telecommunication and electric power industry, together with new telecommunication network technologies and advances in numerical protection, has resulted in the need to reconsider traditional methods of delivering tele-protection schemes and their associated bearer services. Fibre-optic technology is commonly deployed in new telecommunication networks for inter-station communication and utility-owned and public telecommunication networks from third parties are available for protection purposes. (author)

  13. Cathodic Protection Model Facility

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Performs Navy design and engineering of ship and submarine impressed current cathodic protection (ICCP) systems for underwater hull corrosion control and...

  14. DOE groundwater protection strategy

    International Nuclear Information System (INIS)

    Lichtman, S.

    1988-01-01

    EH is developing a DOE-wide Groundwater Quality Protection Strategy to express DOE's commitment to the protection of groundwater quality at or near its facilities. This strategy responds to a September 1986 recommendation of the General Accounting Office. It builds on EPA's August 1984 Ground-Water Protection Strategy, which establishes a classification system designed to protect groundwater according to its value and vulnerability. The purposes of DOE's strategy are to highlight groundwater protection as part of current DOE programs and future Departmental planning, to guide DOE managers in developing site-specific groundwater protection practices where DOE has discretion, and to guide DOE's approach to negotiations with EPA/states where regulatory processes apply to groundwater protection at Departmental facilities. The strategy calls for the prevention of groundwater contamination and the cleanup of groundwater commensurate with its usefulness. It would require long-term groundwater protection with reliance on physical rather than institutional control methods. The strategy provides guidance on providing long-term protection of groundwater resources; standards for new remedial actions;guidance on establishing points of compliance; requirements for establishing classification review area; and general guidance on obtaining variances, where applicable, from regulatory requirements. It also outlines management tools to implement this strategy

  15. Radiation protection in Bolivia

    International Nuclear Information System (INIS)

    Miranda Cuadros, A.A.

    2001-01-01

    Radiation protection in Bolivia has gone through a number of stages. Initially, in the 1970s, the focus was mainly on the analysis of environmental sources resulting from the nuclear tests carried out by France in the Pacific Ocean. Subsequently, the focus switched somewhat to radiation protection in connection with the mining of uranium and in the area of public health. During the third stage, radiation protection in other areas became important as the use of radiation sources was introduced. Finally, during the present -- fourth -- stage, radiation protection regulations are being introduced and mechanisms for the control of radiation sources are being established. (author)

  16. Radiological Protection Act 1970

    International Nuclear Information System (INIS)

    1970-01-01

    This Act provides for the establishment of a Radiological Protection Board to undertake research and advise on protection from radiation hazards. Its functions include provision of advice to Government departments with responsibilities in relation to protection of sectors of the community or the community as a whole against the hazards of ionizing radiation. The Act, which lays down that the Board shall replace certain departments concerned with radiation protection, repeals several Sections of the Radioactive Substances Act 1948 and the Science and Technology Act 1965. (NEA) [fr

  17. Complete rerouting protection

    DEFF Research Database (Denmark)

    Stidsen, Thomas K.; Kjærulff, Peter

    2006-01-01

    Protection of communication against network failures is becoming increasingly important and in this paper we present the most capacity efficient protection method possible, the complete rerouting protection method, when requiring that all communication should be restored in case of a single link...... network failure. We present a linear programming model of the protection method and a column generation algorithm. For 6 real world networks, the minimal restoration overbuild network capacity is between 13% and 78%. We further study the importance of the density of the network, derive analytical bounds...

  18. Techniques in cerebral protection.

    Science.gov (United States)

    Fanelli, Fabrizio; Bezzi, Mario; Boatta, Emanuele; Passariello, Roberto

    2006-10-01

    Carotid angioplasty and stenting is a valid alternative option to conventional carotid endarterectomy in the treatment of carotid artery stenosis. During the stenting process, however, distal embolization can occur with neurological consequences. To avoid this, cerebral protection devices have been introduced. Three principal types of protection system have been developed: distal balloon occlusion, distal filters and proximal protection with or without reversal of flow. As protection devices became the focus of interest by manufactures and physicians, several trials are going on worldwide to analyze the characteristics of each of them and to evaluate their efficacy to reduce the rate of distal embolization.

  19. Protection, interlocks and diagnostics

    CERN Document Server

    Griffiths, S A; White, C; Theed, J E

    2006-01-01

    When designing any power converter it is essential to assess and incorporate adequate protection. The main objective is to offer a solution which is safe, reliable and repairable and that achieves its specification within budget. The level of protection found within each converter varies widely and will depend on the topology employed, its application and rating. This document is a guide to the types of protection engineers should consider mainly when designing power converters, as protection added during construction or after installation will always be expensive.

  20. Radiation protection law

    International Nuclear Information System (INIS)

    Hebert, J.

    1981-01-01

    This article first reviews the general radiation protection law at international and national level, with particular reference to the recommendations of the International Commission on Radiological Protection (ICRP) which, although not mandatory, are nevertheless taken into consideration by international organisations establishing basic radiation protection standards such as the UN, IAEA, NEA and Euratom, at Community level, and by national legislation. These standards are therefore remarkably harmonized. Radiation protection rule applied in France for the different activities and uses of radioactive substances are then described, and finally, a description is given of the regulations governing artificial radioisotopes and radioactive effluents. (NEA) [fr

  1. Investor Protections and Economic Growth

    OpenAIRE

    Haidar, Jamal Ibrahim

    2009-01-01

    Using objective measures of investor protections in 170 countries, I establish that the level of investor protection matters for cross-country differences in GDP growth: countries with stronger protections tend to grow faster than those with poor investor protections.

  2. Protecting the Amazon with protected areas

    Science.gov (United States)

    Walker, Robert; Moore, Nathan J.; Arima, Eugenio; Perz, Stephen; Simmons, Cynthia; Caldas, Marcellus; Vergara, Dante; Bohrer, Claudio

    2009-01-01

    This article addresses climate-tipping points in the Amazon Basin resulting from deforestation. It applies a regional climate model to assess whether the system of protected areas in Brazil is able to avoid such tipping points, with massive conversion to semiarid vegetation, particularly along the south and southeastern margins of the basin. The regional climate model produces spatially distributed annual rainfall under a variety of external forcing conditions, assuming that all land outside protected areas is deforested. It translates these results into dry season impacts on resident ecosystems and shows that Amazonian dry ecosystems in the southern and southeastern basin do not desiccate appreciably and that extensive areas experience an increase in precipitation. Nor do the moist forests dry out to an excessive amount. Evidently, Brazilian environmental policy has created a sustainable core of protected areas in the Amazon that buffers against potential climate-tipping points and protects the drier ecosystems of the basin. Thus, all efforts should be made to manage them effectively. PMID:19549819

  3. Buffer moisture protection system

    International Nuclear Information System (INIS)

    Ritola, J.; Peura, J.

    2013-11-01

    With the present knowledge, bentonite blocks have to be protected from the air relative humidity and from any moisture leakages in the environment that might cause swelling of the bentonite blocks during the 'open' installation phase before backfilling. The purpose of this work was to design the structural reference solution both for the bottom of the deposition hole and for the buffer moisture protection and dewatering system with their integrated equipment needed in the deposition hole. This report describes the Posiva's reference solution for the buffer moisture protection system and the bottom plate on basis of the demands and functional requirements set by long-term safety. The reference solution with structural details has been developed in research work made 2010-2011. The structural solution of the moisture protection system has not yet been tested in practice. On the bottom of the deposition hole a copper plate which protects the lowest bentonite block from the gathered water is installed straight to machined and even rock surface. The moisture protection sheet made of EPDM rubber is attached to the copper plate with an inflatable seal. The upper part of the moisture protection sheet is fixed to the collar structures of the lid which protects the deposition hole in the disposal tunnel. The main function of the moisture protection sheet is to protect bentonite blocks from the leaking water and from the influence of the air humidity at their installation stage. The leaking water is controlled by the dewatering and alarm system which has been integrated into the moisture protection liner. (orig.)

  4. Smart cathodic protection systems

    NARCIS (Netherlands)

    Polder, R.B.; Leggedoor, J.; Schuten, G.; Sajna, S.; Kranjc, A.

    2010-01-01

    Cathodic protection delivers corrosion protection in concrete structures exposed to aggressive environments, e.g. in de-icing salt and marine climates. Working lives of a large number of CP systems are at least more than 13 years and probably more than 25 years, provided a minimum level of

  5. Complete Rerouting Protection

    DEFF Research Database (Denmark)

    Stidsen, Thomas K.; Kjærulff, Peter

    2005-01-01

    In this paper we present a new protection method: Complete Rerouting. This is the most capacity e cient protection method for circuit switched networks and it is, to the best of our knowledge, the first time it has been described. We implement a column generation algorithm and test the performance...

  6. Protected urban planet

    NARCIS (Netherlands)

    Pereira Roders, A.R.; Veldpaus, L.; Verbruggen, R.C.

    2012-01-01

    PUP, abbreviation to Protected Urban Planet, is the first tool developed for visualizing, mapping and contributing to information exchange on the evolution of protected urban areas worldwide. Besides locating them, it also provides communities with means to disseminate and raise awareness for their

  7. Protective Health Education

    Science.gov (United States)

    Aydin, Ganime

    2016-01-01

    Problem Statement: As a result of wars, starvation, traffic accidents, homicide, infectious diseases, insufficient adult protection, migration, and inadequate legal reforms the mortality rate of children has become a serious problem in the world. Protective health education contributes to a child's physical and social health. In this case, the…

  8. Regulations in radiation protection

    International Nuclear Information System (INIS)

    1986-01-01

    On the occasion of the twenty fifth anniversary of the Dutch Society for Radiation Protection, a symposium was held about Regulations in Radiation Protection. The program consisted of six contributions of which four are included in this publication. The posters presented are published in NVS-nieuws, 1985, vol. 11(5). (G.J.P.)

  9. Individual protections and ergonomics

    International Nuclear Information System (INIS)

    2000-01-01

    The object of this conference was the protective clothing against radioactive contamination. The regulatory frame, the physiological constraints and the human factor are the different aspects studied through the conference with a constant objective, the optimization of radiation protection. (N.C.)

  10. Environmental protection Implementation Plan

    International Nuclear Information System (INIS)

    Holland, R. C.

    1999-01-01

    This ''Environmental Protection Implementation Plan'' is intended to ensure that the environmental program objectives of Department of Energy Order 5400.1 are achieved at SNL/California. This document states SNL/California's commitment to conduct its operations in an environmentally safe and responsible manner. The ''Environmental Protection Implementation Plan'' helps management and staff comply with applicable environmental responsibilities

  11. The Radiation Protection Act

    International Nuclear Information System (INIS)

    Persson, L.

    1989-01-01

    The new Radiation Protection Act (1988:220) entered into force in Sweden on July 1st, 1988. This book presents the Act as well as certain regulations connected to it. As previously, the main responsibility for public radiation protection will rest with one central radiation protection authority. According to the 1988 Act, the general obligations with regard to radiation protection will place a greater responsibility than in the past on persons carrying out activities involving radiation. Under the act, it is possible to adjust the licensing and supervisory procedures to the level of danger of the radiation source and the need for adequate competence, etc. The Act recognises standardised approval procedures combined with technical regulations for areas where the risks are well known. The Act contains several rules providing for more effective supervision. The supervising authority may in particular decide on the necessary regulations and prohibitions for each individual case. The possibilities of using penal provisions have been extended and a rule on the mandatory execution of orders has been introduced. The Ordinance on Radiation Protection (1988:293) designates the National Institute of Radiation Protection (SSI) as the central authority referred to in the Radiation Protection Act. The book also gives a historic review of radiation protection laws in Sweden, lists regulations issued by SSI and presents explanations of radiation effects and international norms in the area. (author)

  12. Electroplating offers embrittlement protection

    Science.gov (United States)

    Daniels, C. M., Jr.

    1970-01-01

    Thin copper electrodeposited layer protects metal parts in environments with which they may be incompatible. Originally developed for main engine of Space Shuttle where high strength nickle alloy bellows must operate in high-pressure hydrogen, technique protects nickel and is unaffected by forming process or subsequent heat treatment and preinstallation processing.

  13. Practical radiation protection

    International Nuclear Information System (INIS)

    Brouwer, G.; Van den Eijnde, J.H.G.M.

    1997-01-01

    This textbook aims at providing sufficient knowledge and insight to carry out correctly radiation protection activities and operations. The subjects are appropriate for the training of radiation protection experts for the levels 5A (encapsulated sources, X rays) and 5B (open sources, laboratory activities)

  14. Consumer rights and protections

    Science.gov (United States)

    ... care consumer rights; Rights of the health care consumer ... RIGHTS AND PROTECTIONS Here are ways that the health care law protects consumers. You must be covered, even if you have a pre-existing condition. No insurance plan can reject you, ...

  15. Military radiation protection

    International Nuclear Information System (INIS)

    Harrison, J.

    1993-01-01

    The Ministry of Defence and the military in particular have a very strong commitment to radiation protection of personnel in war and peace. MOD endeavours to do better all the time because it is essential that the armed forces have the confidence to fulfil their role and this is best achieved by providing them with the best possible protection irrespective of the hazard. (author)

  16. Respirators and protective clothing

    International Nuclear Information System (INIS)

    1967-01-01

    The basic object in the use of protective clothing and equipment is to prevent contamination of the skin and to prevent inhalation and ingestion of radioactive isotopes or other toxic materials. This book is a guide to deciding the kind and quantity of protective equipment needed for a particular type of laboratory or operation.

  17. Optimisation of radiation protection

    International Nuclear Information System (INIS)

    1988-01-01

    Optimisation of radiation protection is one of the key elements in the current radiation protection philosophy. The present system of dose limitation was issued in 1977 by the International Commission on Radiological Protection (ICRP) and includes, in addition to the requirements of justification of practices and limitation of individual doses, the requirement that all exposures be kept as low as is reasonably achievable, taking social and economic factors into account. This last principle is usually referred to as optimisation of radiation protection, or the ALARA principle. The NEA Committee on Radiation Protection and Public Health (CRPPH) organised an ad hoc meeting, in liaison with the NEA committees on the safety of nuclear installations and radioactive waste management. Separate abstracts were prepared for individual papers presented at the meeting

  18. Introduction to Machine Protection

    CERN Document Server

    Schmidt, R

    2016-01-01

    Protection of accelerator equipment is as old as accelerator technology and was for many years related to high-power equipment. Examples are the protection of powering equipment from overheating (magnets, power converters, high-current cables), of superconducting magnets from damage after a quench and of klystrons. The protection of equipment from beam accidents is more recent, although there was one paper that discussed beam-induced damage for the SLAC linac (Stanford Linear Accelerator Center) as early as in 1967. It is related to the increasing beam power of high-power proton accelerators, to the emission of synchrotron light by electron-positron accelerators and to the increase of energy stored in the beam. Designing a machine protection system requires an excellent understanding of accelerator physics and operation to anticipate possible failures that could lead to damage. Machine protection includes beam and equipment monitoring, a system to safely stop beam operation (e.g. dumping the beam or stopping ...

  19. Wake Shield Target Protection

    International Nuclear Information System (INIS)

    Valmianski, Emanuil I.; Petzoldt, Ronald W.; Alexander, Neil B.

    2003-01-01

    The heat flux from both gas convection and chamber radiation on a direct drive target must be limited to avoid target damage from excessive D-T temperature increase. One of the possibilities of protecting the target is a wake shield flying in front of the target. A shield will also reduce drag force on the target, thereby facilitating target tracking and position prediction. A Direct Simulation Monte Carlo (DSMC) code was used to calculate convection heat loads as boundary conditions input into ANSYS thermal calculations. These were used for studying the quality of target protection depending on various shapes of shields, target-shield distance, and protective properties of the shield moving relative to the target. The results show that the shield can reduce the convective heat flux by a factor of 2 to 5 depending on pressure, temperature, and velocity. The protective effect of a shield moving relative to the target is greater than the protective properties of a fixed shield. However, the protective effect of a shield moving under the drag force is not sufficient for bringing the heat load on the target down to the necessary limit. Some other ways of diminishing heat flux using a protective shield are discussed

  20. Optimization in radiological protection

    International Nuclear Information System (INIS)

    Acosta Perez, Clarice de Freitas

    1996-01-01

    The optimization concept in radiation protection is, in its essence, practical. In each aspect that we deal with the man, it is necessary to take frequent decisions such as: what is the protection level to be pursued, since the protection levels under consideration provide doses lower than the appropriate annual limits. The optimization gives a basic framework of the minding that is appropriate to conduct to a balance kind of the resources available for the protection and protection level obtained against a multitude of factors and constrains in a manner to obtain the best result. In this work, was performed the optimization, from the radiation protection point of view, of a facility project who enclose two shielded hot cells where will be handled UO 2 small plate with 50% of U-235 burn-up, irradiated in the research swimming pool reactor, IEA-R1. To obtain this goal were specified the relevant factors and criteria, were applied the main techniques used in a decision-making in radiological protection, presently adopted and was performed a sensibility study of the factors and criteria used in this work. In order to obtain a greater agility in applying the techniques for decision-making was developed a micro computer program. (author)

  1. Innovative protection cookbook

    International Nuclear Information System (INIS)

    Lambert, D.L.

    2002-01-01

    Full text: A large portion of the international community relies on expert judgment and military expertise to form the protection strategy of weapons-grade nuclear materials. This approach can be less than effectual for two primary reasons. First, much of the nuclear material that needs the most protection is not in a static form and located in easily secured storage locations. Some of the most effective measures for protection of in-process material are administrative and procedural in nature. There are basic protection differences between countries just like there are differences in baking bread. However, the necessary steps to produce bread are basically the same around the world. Certain ingredients are required to give it flavor, make it rise, and have a certain texture. So it is in the development of protection systems. A measure of intrusion detection, some access controls and searches, a pinch of computerized accounting, a layer of delay barriers, and a smidgen of armed response--and the basis for a 'cookbook' is born. Any and all protection systems can begin with simple elements that can be just as effective as the most sophisticated modern components. This paper will describe some of those simple, but effective, innovative protection measures. (author)

  2. Shanghai's Huangpu Riverbank Redevelopment Beyond World Expo 2010

    NARCIS (Netherlands)

    Chen, Y.; Tu, Q.; Su, N.

    2014-01-01

    Event-led regeneration has become part of a deliberate urban policy strategy to position the host cities on the world agenda. Mega events such as the world expo need different facilities and services for an efficient operation of the expo during those three or six months. Most cities stressed

  3. Theoretical investigation on discharge-induced river-bank erosion

    NARCIS (Netherlands)

    Mosselman, E.

    1989-01-01

    Bank erosion is incorporated in one-dimensional and two-dimensional horizontal models for river morphology. The banks are assumed to consist of a fraction of cohesive material, which becomes washload after being eroded, and a fraction of granular material, with the same properties as the material of

  4. The riverbank, the seashore and the wilderness: Miriam, liberation ...

    African Journals Online (AJOL)

    2017-07-25

    Jul 25, 2017 ... feminist perspective it is argued that this style of non-violent, faithful prophetic witness has a ..... now shared by Yahweh and the military leader, the king. For the first ...... increasingly autocratic, and increasingly unpopular, rule.

  5. Feasibility Study of Improved Methods for Riverbank Stabilization

    Science.gov (United States)

    1964-11-01

    often placed under and near the structure to prevent or reduce undercut- ting. P:.acement Conditions and Meth ods: All probable clima tic, foundation...inch thick lining on several canals cost from $0. 08 to $0. 09 per square foot including labor , equipment, and materials, but not including subgrade...would f eed fou r presses . The cost figures below take into account ( 1) labor of three men per s hift wit h one general supervi sor, (2

  6. Ethics and radiation protection

    International Nuclear Information System (INIS)

    Hansson, Sven Ove

    2007-01-01

    Some of the major problems in radiation protection are closely connected to issues that have a long, independent tradition in moral philosophy. This contribution focuses on two of these issues. One is the relationship between the protection of individuals and optimisation on the collective level, and the other is the relative valuation of future versus immediate damage. Some of the intellectual tools that have been developed by philosophers can be useful in radiation protection. On the other hand, philosophers have much to learn from radiation protectors, not least when it comes to finding pragmatic solutions to problems that may be intractable in principle

  7. PROTECTION OF THREATENED WITNESSES

    Directory of Open Access Journals (Sweden)

    Nadia Claudia CANTEMIR-STOICA

    2016-05-01

    Full Text Available First, I wish to make a presentation of historically institution and subsequently parallels between past and current regulators to expose whether the legislature has reached desire - namely ensuring effective protection of witnesses threatened and vulnerable. Also, I decided to analyze the topic from the perspective of the criminal procedural provisions of Law 682/2002 and witness protection, which are republished to expose the conditions and criteria by which to ensure this status. I also want to present besides theoretical and practical ways in which the National Office for Witness Protection gives effective legal provisions. Not least, I will bring criticism of current regulation and not by law ferenda proposals.

  8. Ethics and radiation protection

    Energy Technology Data Exchange (ETDEWEB)

    Hansson, Sven Ove [Department of Philosophy and the History of Technology, Royal Institute of Technology (KTH), Teknikringen 78 B, 2tr, SE-100 44 Stockholm (Sweden)

    2007-06-01

    Some of the major problems in radiation protection are closely connected to issues that have a long, independent tradition in moral philosophy. This contribution focuses on two of these issues. One is the relationship between the protection of individuals and optimisation on the collective level, and the other is the relative valuation of future versus immediate damage. Some of the intellectual tools that have been developed by philosophers can be useful in radiation protection. On the other hand, philosophers have much to learn from radiation protectors, not least when it comes to finding pragmatic solutions to problems that may be intractable in principle.

  9. Selective protection. Selektivschutz

    Energy Technology Data Exchange (ETDEWEB)

    1983-01-01

    TC 2, ''Transmission and distribution of electrical energy'', held its meeting on selective protection on 16/17 March 1983 at Nuremberg. The main reason for the development of computerized protective systems is their ability to solve a wide range of tasks using only a few, high-integrated systems, i.e. on the software side instead of the hardware side. At the meeting, experts from industrial companies and public utilities reviewed the status and trends of protective systems engineering. Scientific studies and operating experience were presented. Separate abstracts were prepared for 2 papers in this conference proceedings volume.

  10. Radiation protective clothing

    International Nuclear Information System (INIS)

    Fujinuma, Tadashi; Tamura, Shoji; Ijiri, Yasuo.

    1988-01-01

    Purpose: To obtain radiation protective clothings of excellent workability and durability. Constitution: Protective clothings of the present invention comprise shielding materials for the upper-half of the body having lead foils laminated on one surface and shielding materials for the lower-half of the body a resin sheet containing inorganic powders of high specific gravity. Such protective clothings have a frexibility capable of followings after the movement of the upper-half body and easily follow after the movement such as acute bending of the body near the waste in the lower-half body. (Kamimura, M.)

  11. Fall Protection Introduction, #33462

    Energy Technology Data Exchange (ETDEWEB)

    Chochoms, Michael [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-06-23

    The proper use of fall prevention and fall protection controls can reduce the risk of deaths and injuries caused by falls. This course, Fall Protection Introduction (#33462), is designed as an introduction to various types of recognized fall prevention and fall protection systems at Los Alamos National Laboratory (LANL), including guardrail systems, safety net systems, fall restraint systems, and fall arrest systems. Special emphasis is given to the components, inspection, care, and storage of personal fall arrest systems (PFASs). This course also presents controls for falling object hazards and emergency planning considerations for persons who have fallen.

  12. Protective head of sensors

    International Nuclear Information System (INIS)

    Liska, K.; Anton, P.

    1987-01-01

    The discovery concerns the protective heads of diagnostic assemblies of nuclear power plants for conductors of the sensors from the fuel and control parts of the said assemblies. A detailed description is presented of the design of the protective head which, as compared with the previous design, allows quick and simple assembly with reduced risk of damaging the sensors. The protective head may be used for diagnostic assemblies both in power and in research reactors and it will be used for WWER reactor assemblies. (A.K.). 3 figs

  13. Radiation protection; Proteccion Radiologica

    Energy Technology Data Exchange (ETDEWEB)

    Ures Pantazi, M [Universidad de la Republica, Facultad de Quimica (Uruguay)

    1994-12-31

    This work define procedures and controls about ionizing radiations. Between some definitions it found the following topics: radiation dose, risk, biological effects, international radioprotection bodies, workers exposure, accidental exposure, emergencies and radiation protection.

  14. Physics for radiation protection

    CERN Document Server

    Martin, James E

    2013-01-01

    A much-needed working resource for health physicists and other radiation protection professionals, this volume presents clear, thorough, up-to-date explanations of the basic physics necessary to address real-world problems in radiation protection. Designed for readers with limited as well as basic science backgrounds, Physics for Radiation Protection emphasizes applied concepts and carefully illustrates all topics through examples as well as practice problems. Physics for Radiation Protection draws substantially on current resource data available for health physics use, providing decay schemes and emission energies for approximately 100 of the most common radionuclides encountered by practitioners. Excerpts of the Chart of the Nuclides, activation cross sections, fission yields, fission-product chains, photon attenuation coefficients, and nuclear masses are also provided.

  15. Ingredients of protection engineering

    International Nuclear Information System (INIS)

    Latorre, V.R.; Spogen, L.R. Jr.

    1974-01-01

    The protection of an electronic/electrical system from an electromagnetic environment requires a systematic approach and a clear and concise identification of the data requirements and tools (analytical and experimental) for the vulnerability assessment. In this paper, a basic protection engineering concept is developed. The concept is general and therefore applicable to many systems. It consists of five stages: (1) systems analysis, (2) subsystem degradation, (3) interaction and coupling, (4) protection trade-off analysis, and (5) fabrication and operation. A detailed discussion of all functions performed in each stage is described; the input data to these functions identified; and means (tools) by which the functional operations are satisfied are summarized. Simple examples are used throughout the paper to illustrate the definitions of various functions and to emphasize the iterative process required in arriving at a protection scheme. (U.S.)

  16. Protection without Protectionism.

    Science.gov (United States)

    Saur, Ricardo A. C.

    1979-01-01

    Presents a Third World approach to transborder data flow. Suggests developing local capacities for computer power, confining data flow within borders, and providing protection for local development efforts. (PD)

  17. Radiation protection in brachytherapy

    International Nuclear Information System (INIS)

    Benitez, Manuel

    1996-02-01

    It covers technical procedures in medical applications for cancer treatment. Radiation protection principles in brachytherapy. Medical uses in therapy for Sr-90, Cs-137, Co-60, Ra-226, Ir-192, Au-198, Bi-214, Pb-214. (The author)

  18. Tritium protective clothing

    International Nuclear Information System (INIS)

    Fuller, T.P.; Easterly, C.E.

    1979-06-01

    Occupational exposures to radiation from tritium received at present nuclear facilities and potential exposures at future fusion reactor facilities demonstrate the need for improved protective clothing. Important areas relating to increased protection factors of tritium protective ventilation suits are discussed. These areas include permeation processes of tritium through materials, various tests of film permeability, selection and availability of suit materials, suit designs, and administrative procedures. The phenomenological nature of film permeability calls for more standardized and universal test methods, which would increase the amount of directly useful information on impermeable materials. Improvements in suit designs could be expedited and better communicated to the health physics community by centralizing devlopmental equipment, manpower, and expertise in the field of tritium protection to one or two authoritative institutions

  19. Radiation Protection: introduction

    International Nuclear Information System (INIS)

    Loos, M.

    2005-01-01

    The abstract gives an overview and introduction to the activities of SCK-CEN's Radiation Protection department. Main strategic developments and achievements in the field of life sciences, policy supports and medical applications are summarised

  20. Radiation protection in medicine

    International Nuclear Information System (INIS)

    Vano, E.; Holmberg, O.; Perez, M. R.; Ortiz, P.

    2016-01-01

    Diagnostic, interventional and therapeutic used of ionizing radiation are beneficial for hundreds of millions of people each year by improving health care and saving lives. In March 2001, the first International Conference on the Radiological Protection of Patients was held in Malaga, Spain, which led to an international action plan for the radiation protection of patients. Ten years after establishing the international action plan, the International Conference on Radiation Protection in Medicine: Setting the Scene for the Next Decade was held in Bonn, Germany, in December 2012. the main outcome of this conference was the so called Bonn Call for Action that identifies then priority actions to enhance radiation protection in medicine for the next decade. The IAEA and WHO are currently working in close cooperation to foster and support the implementation of these ten priority actions in Member States, but their implementation requires collaboration of national governments, international agencies, researchers, educators, institutions and professional associations. (Author)

  1. Water Quality Protection Charges

    Data.gov (United States)

    Montgomery County of Maryland — The Water Quality Protection Charge (WQPC) is a line item on your property tax bill. WQPC funds many of the County's clean water initiatives including: • Restoration...

  2. Radiation protection in medicine

    Energy Technology Data Exchange (ETDEWEB)

    Vano, E.; Holmberg, O.; Perez, M. R.; Ortiz, P.

    2016-08-01

    Diagnostic, interventional and therapeutic used of ionizing radiation are beneficial for hundreds of millions of people each year by improving health care and saving lives. In March 2001, the first International Conference on the Radiological Protection of Patients was held in Malaga, Spain, which led to an international action plan for the radiation protection of patients. Ten years after establishing the international action plan, the International Conference on Radiation Protection in Medicine: Setting the Scene for the Next Decade was held in Bonn, Germany, in December 2012. the main outcome of this conference was the so called Bonn Call for Action that identifies then priority actions to enhance radiation protection in medicine for the next decade. The IAEA and WHO are currently working in close cooperation to foster and support the implementation of these ten priority actions in Member States, but their implementation requires collaboration of national governments, international agencies, researchers, educators, institutions and professional associations. (Author)

  3. Radiation protection and monitoring

    International Nuclear Information System (INIS)

    Thomas, P.

    1982-01-01

    The present paper deals with the following topics: - Radiological quantities and units - Principles of radiological protection - Limits of doses and activity uptake - Activity discharges and monitoring - Radiation exposure and its calculation - Environmental monitoring - Personnel dosimetry. (orig./RW)

  4. Pollinator Protection Strategic Plan

    Science.gov (United States)

    Developed by EPA, this ensures that pesticide risk assessments and risk management decisions use best available information and scientific methods, and full evaluation of pollinator protection when making registration decisions.

  5. Personal Protective Equipment

    National Research Council Canada - National Science Library

    1998-01-01

    ... of personal protective equipment A safety program for new employees is a necessary part of any orientation program An on-going safety program should be used to motivate employees to continue to use...

  6. Power system protection

    International Nuclear Information System (INIS)

    Venkata, S.S.; Damborg, M.J.; Jampala, A.K.

    1991-01-01

    Power systems of the 21st century will be more modern, and complex, utilizing the latest available technologies. At the same time, generating plants will have to operate with minimal spinning margins and energy transportation has to take place at critical levels due to environmental and economical constraints. These factors dictate that the power systems be protected with optimum sensitivity, selectivity and time of operation to assure maximum reliability, and security at minimal cost. With an increasing role played by digital computers in every aspect of protection, it is important to take a critical and fresh look at the art and science of relaying and protection. The main objective of this paper is to review the past, present and future of power system protection from a software point of view

  7. Radiation Protection Group

    CERN Document Server

    2006-01-01

    The Radioactive Waste Section of the Radiation Protection Group wishes to inform you that the Radioactive Waste Treatment Centre will be closed on the afternoon of Tuesday 19 December 2006. Thank-you for your understanding.

  8. Asbestos: Protect Your Family

    Science.gov (United States)

    ... Related Topics: Asbestos Contact Us Share Protect Your Family How to Identify Materials That May Contain Asbestos ... Improper removal may actually increase your and your family’s exposure to asbestos fibers. Top of Page Asbestos ...

  9. Software for radiation protection

    International Nuclear Information System (INIS)

    Graffunder, H.

    2002-01-01

    The software products presented are universally usable programs for radiation protection. The systems were designed in order to establish a comprehensive database specific to radiation protection and, on this basis, model in programs subjects of radiation protection. Development initially focused on the creation of the database. Each software product was to access the same nuclide-specific data; input errors and differences in spelling were to be excluded from the outset. This makes the products more compatible with each other and able to exchange data among each other. The software products are modular in design. Functions recurring in radiation protection are always treated the same way in different programs, and also represented the same way on the program surface. The recognition effect makes it easy for users to familiarize with the products quickly. All software products are written in German and are tailored to the administrative needs and codes and regulations in Germany and in Switzerland. (orig.) [de

  10. Radiation protection standards

    International Nuclear Information System (INIS)

    Fitch, J.

    1983-11-01

    Topics covered include biological radiation effects, radiation protection principles, recommendations of the ICRP and the National Health and Medical Research Council, and dose limits for individuals, particularly the limit applied to the inhalation of radon daughters

  11. Tritium protective clothing

    Energy Technology Data Exchange (ETDEWEB)

    Fuller, T. P.; Easterly, C. E.

    1979-06-01

    Occupational exposures to radiation from tritium received at present nuclear facilities and potential exposures at future fusion reactor facilities demonstrate the need for improved protective clothing. Important areas relating to increased protection factors of tritium protective ventilation suits are discussed. These areas include permeation processes of tritium through materials, various tests of film permeability, selection and availability of suit materials, suit designs, and administrative procedures. The phenomenological nature of film permeability calls for more standardized and universal test methods, which would increase the amount of directly useful information on impermeable materials. Improvements in suit designs could be expedited and better communicated to the health physics community by centralizing devlopmental equipment, manpower, and expertise in the field of tritium protection to one or two authoritative institutions.

  12. Expeditionary Warfare- Force Protection

    National Research Council Canada - National Science Library

    Higgins, Eric

    2004-01-01

    In 2003, the Systems Engineering and Analysis students were tasked to develop a system of systems conceptual solution to provide force protection for the Sea Base conceptualized in the 2002 Expeditionary Warfare study...

  13. Protected Objects in Java

    DEFF Research Database (Denmark)

    Løvengreen, Hans Henrik; Schwarzer, Jens Christian

    1998-01-01

    We present an implementation of Ada 95's notion of protected objects in Java. The implementation comprises a class library supporting entry queues and a (pre-) compiler translating slightly decorated Java classes to pure Java classes utilizing the library.......We present an implementation of Ada 95's notion of protected objects in Java. The implementation comprises a class library supporting entry queues and a (pre-) compiler translating slightly decorated Java classes to pure Java classes utilizing the library....

  14. Radiation protection housing

    Energy Technology Data Exchange (ETDEWEB)

    Maier, A

    1975-04-10

    The radiation protection housing consists of a foot rim with castor swivel wheels, a tubular frame tapering off at the top, and a crown. In the upper part of the tubular frame a lead glass window is permanently installed. The sides are covered with radiation attenuating curtains of leaded rubber. The housing has the shape of a truncated pyramid which can be dismantled into its constituent parts. It is used for protection from radiation encountered in X-ray facilities in dental radiology.

  15. Advanced worker protection system

    International Nuclear Information System (INIS)

    Caldwell, B.; Duncan, P.; Myers, J.

    1995-01-01

    The Department of Energy (DOE) is in the process of defining the magnitude and diversity of Decontamination and Decommissioning (D ampersand D) obligations at its numerous sites. The DOE believes that existing technologies are inadequate to solve many challenging problems such as how to decontaminate structures and equipment cost effectively, what to do with materials and wastes generated, and how to adequately protect workers and the environment. Preliminary estimates show a tremendous need for effective use of resources over a relatively long period (over 30 years). Several technologies are being investigated which can potentially reduce D ampersand D costs while providing appropriate protection to DOE workers. The DOE recognizes that traditional methods used by the EPA in hazardous waste site clean up activities are insufficient to provide the needed protection and worker productivity demanded by DOE D ampersand D programs. As a consequence, new clothing and equipment which can adequately protect workers while providing increases in worker productivity are being sought for implementation at DOE sites. This project will result in the development of an Advanced Worker Protection System (AWPS). The AWPS will be built around a life support backpack that uses liquid air to provide cooling as well as breathing gas to the worker. The backpack will be combined with advanced protective garments, advanced liquid cooling garment, respirator, communications, and support equipment to provide improved worker protection, simplified system maintenance, and dramatically improve worker productivity through longer duration work cycles. Phase I of the project has resulted in a full scale prototype Advanced Worker Protection Ensemble (AWPE, everything the worker will wear), with sub-scale support equipment, suitable for integrated testing and preliminary evaluation. Phase II will culminate in a full scale, certified, pre-production AWPS and a site demonstration

  16. Radiation protection forum

    International Nuclear Information System (INIS)

    Cabral, W.

    2010-01-01

    The National Director of the Nuclear Regulatory Authority and Radiation Protection of Uruguay in the first forum for radiation protection set out the following themes: activity of regulatory body, radiation safety, physical security, safeguards, legal framework, committed substantive program, use of radiation, risks and benefits, major sources of radiation, the national regulatory framework, national inventory of sources, inspections, licensing, import and export of sources control , radioactive transport, materials safety, agreements, information and teaching, radiological emergencies and prompt response.

  17. Manual of Radiation Protection

    International Nuclear Information System (INIS)

    Gambini, D.J.; Granier, R.; Boisserie, G.

    1992-01-01

    This manual explains the principles and practice of radiation protection for those whose work in research, in the field of medicine or in the industry requires the use of radiation sources. It provides the information radiation users need to protect themselves and others and to understand and comply with international recommendations, regulations and legislation regarding the use of radionuclides and radiation machines. It is designed to teach a wide audience of doctors, biologists, research scientists, technicians, engineers, students and others

  18. Radiation protection instrument 1993

    International Nuclear Information System (INIS)

    1993-04-01

    The Radiation Protection Instrument, 1993 (Legislative Instrument 1559) prescribes the powers and functions of the Radiation Protection Board established under the Ghana Atomic Energy Commission by the Atomic Energy Commission (Amendment) Law, 1993 (P.N.D.C. Law 308). Also included in the Legislative Instrument are schedules on control and use of ionising radiation and radiation sources as well as procedures for notification, licensing and inspection of ionising radiation facilities. (EAA)

  19. Laser radiation protection

    International Nuclear Information System (INIS)

    Pantelic, D.; Muric, B.; Vasiljevic, D.

    2011-01-01

    We have presented the effects of laser radiation on human organism, with special emphasize on eye as the most sensitive organ. It was pointed-out that there are many parameters that should be taken into account when determining the level of protection from laser light. In that respect it is important to be aware of international standards that regulate this area. In addition, we have described a new material which efficiently protects human eye, by formation of microlens and carbonization. [sr

  20. Radiation protection philosophy alters

    International Nuclear Information System (INIS)

    Firmin, G.

    1977-01-01

    Two significant events that have taken place this year in the field of radiation protection are reported. New SI units have been proposed (and effectively adopted), and the ICRP has revised its recommendations. Changes of emphasis in the latest recommendations (ICRP Publication 26) imply an altered radiation protection philosophy, in particular the relation of dose limits to estimates of average risk, an altered view of the critical organ approach and a new attitude to genetic dose to the population. (author)

  1. Advanced worker protection system

    Energy Technology Data Exchange (ETDEWEB)

    Caldwell, B.; Duncan, P.; Myers, J.

    1995-12-01

    The Department of Energy (DOE) is in the process of defining the magnitude and diversity of Decontamination and Decommissioning (D&D) obligations at its numerous sites. The DOE believes that existing technologies are inadequate to solve many challenging problems such as how to decontaminate structures and equipment cost effectively, what to do with materials and wastes generated, and how to adequately protect workers and the environment. Preliminary estimates show a tremendous need for effective use of resources over a relatively long period (over 30 years). Several technologies are being investigated which can potentially reduce D&D costs while providing appropriate protection to DOE workers. The DOE recognizes that traditional methods used by the EPA in hazardous waste site clean up activities are insufficient to provide the needed protection and worker productivity demanded by DOE D&D programs. As a consequence, new clothing and equipment which can adequately protect workers while providing increases in worker productivity are being sought for implementation at DOE sites. This project will result in the development of an Advanced Worker Protection System (AWPS). The AWPS will be built around a life support backpack that uses liquid air to provide cooling as well as breathing gas to the worker. The backpack will be combined with advanced protective garments, advanced liquid cooling garment, respirator, communications, and support equipment to provide improved worker protection, simplified system maintenance, and dramatically improve worker productivity through longer duration work cycles. Phase I of the project has resulted in a full scale prototype Advanced Worker Protection Ensemble (AWPE, everything the worker will wear), with sub-scale support equipment, suitable for integrated testing and preliminary evaluation. Phase II will culminate in a full scale, certified, pre-production AWPS and a site demonstration.

  2. Protection Coordination in Electrical Substation Part-2 Unit Protections (Differential and Distance Protection)

    OpenAIRE

    TÜR, Mehmet Rida; Shobole, Abdulfetah; Baysal, Mustafa; Wadi, Mohammed

    2018-01-01

    Power systemsmust be protected against faults to ensure quality and reliable generation,transmission and distribution of power systems. Power system protection is providedby the protection relays. This paper is the second part of the ProtectionCoordination study for the Siddik Kardesler Substation. The protection fortransmission lines, transformer, bus bars and customer feeders is provided byovercurrent protection, differential and distance protection schemes. In thispaper, issues related wit...

  3. Protection of Levees against Beavers

    Science.gov (United States)

    Kozłowski, Wojciech; Balawejder, Adam

    2017-10-01

    Beavers are a protected species, so the levees must be properly protected so as not to harm the beavers and protect the levees from the destruction caused by these animals. This protection requires the use of bentonite mats as shaft seals, and wire mesh. Recently, such new protection structures began to be used successfully.

  4. Advanced worker protection system

    Energy Technology Data Exchange (ETDEWEB)

    Caldwell, B.; Duncan, P.; Myers, J. [Oceaneering Space Systems, Houston, TX (United States)

    1995-10-01

    The Department of Energy (DOE) is in the process of defining the magnitude and diversity of Decontamination and Decommissioning (D&D) obligations at its numerous sites. The DOE believes that existing technologies are inadequate to solve many challenging problems such as how to decontaminate structures and equipment cost effectively, what to do with materials and wastes generated, and how to adequately protect workers and the environment. Preliminary estimates show a tremendous need for effective use of resources over a relatively long period (over 30 years). Several technologies are being investigated which can potentially reduce D&D costs while providing appropriate protection to DOE workers. The DOE recognizes that traditional methods used by the EPA in hazardous waste site clean up activities are insufficient to provide the needed protection and worker productivity demanded by DOE D&D programs. As a consequence, new clothing and equipment which can adequately protect workers while providing increases in worker productivity are being sought for implementation at DOE sites. This project describes the development of an Advanced Worker Protection System (AWPS) which will include a life-support backpack with liquid air for cooling and as a supply of breathing gas, protective clothing, respirators, communications, and support equipment.

  5. Advanced worker protection system

    International Nuclear Information System (INIS)

    Caldwell, B.; Duncan, P.; Myers, J.

    1995-01-01

    The Department of Energy (DOE) is in the process of defining the magnitude and diversity of Decontamination and Decommissioning (D ampersand D) obligations at its numerous sites. The DOE believes that existing technologies are inadequate to solve many challenging problems such as how to decontaminate structures and equipment cost effectively, what to do with materials and wastes generated, and how to adequately protect workers and the environment. Preliminary estimates show a tremendous need for effective use of resources over a relatively long period (over 30 years). Several technologies are being investigated which can potentially reduce D ampersand D costs while providing appropriate protection to DOE workers. The DOE recognizes that traditional methods used by the EPA in hazardous waste site clean up activities are insufficient to provide the needed protection and worker productivity demanded by DOE D ampersand D programs. As a consequence, new clothing and equipment which can adequately protect workers while providing increases in worker productivity are being sought for implementation at DOE sites. This project describes the development of an Advanced Worker Protection System (AWPS) which will include a life-support backpack with liquid air for cooling and as a supply of breathing gas, protective clothing, respirators, communications, and support equipment

  6. An experimental modeling of trinomial bioengineering- crp, rDNA, and transporter engineering within single cell factory for maximizing two-phase bioreduction.

    Science.gov (United States)

    Basak, Souvik; Ghosh, Sumanta Kumar; Punetha, Vinay Deep; Aphale, Ashish N; Patra, Prabir K; Sahoo, Nanda Gopal

    2017-02-01

    A carbonyl reductase (cr) gene from Candida glabrata CBS138 has been heterologously expressed in cofactor regenerating E. coli host to convert Ethyl-4-chloro-3-oxobutanoate (COBE) into Ethyl-4-chloro-3-hydroxybutanoate (CHBE). The CR enzyme exhibited marked velocity at substrate concentration as high as 363mM with highest turnover number (112.77±3.95s -1 ). Solitary recombineering of such catalytic cell reproduced CHBE 161.04g/L per g of dry cell weight (DCW). Introduction of combinatorially engineered crp (crp*, F136I) into this heterologous E. coli host yielded CHBE 477.54g/L/gDCW. Furthermore, using nerolidol as exogenous cell transporter, the CHBE productivity has been towered to 710.88g/L/gDCW. The CHBE production has thus been upscaled to 8-12 times than those reported so far. qRT-PCR studies revealed that both membrane efflux channels such as acrAB as well as ROS scavenger genes such as ahpCF have been activated by engineering crp. Moreover, membrane protecting genes such as manXYZ together with solvent extrusion associated genes such as glpC have been upregulated inside mutant host. Although numerous proteins have been investigated to convert COBE to CHBE; this is the first approach to use engineering triad involving crp engineering, recombinant DNA engineering and transporter engineering together for improving cell performance during two-phase biocatalysis. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Protective effects of different marigold (Calendula officinalis L.) and rosemary cream preparations against sodium-lauryl-sulfate-induced irritant contact dermatitis.

    Science.gov (United States)

    Fuchs, S M; Schliemann-Willers, S; Fischer, T W; Elsner, P

    2005-01-01

    In the present study, we evaluated the protective action of cream preparations containing seven different types of marigold and rosemary extracts in vivo in healthy volunteers with experimentally induced irritant contact dermatitis (ICD). Marigold and rosemary extracts in base cream DAC (Deutscher Arzneimittel-Codex = German Pharmaceutical Codex) were tested in a 4-day repetitive irritation test using sodium lauryl sulfate. The effect was evaluated visually and quantified by noninvasive bioengineering methods, namely chromametry and tewametry. When the test products were applied parallel to the induction period of ICD, a statistically significant protective effect of all cream preparations was observed by all methods. This effect, although not statistically significant, was superior to control by undyed marigold und faradiol ester-enriched extracts in chromametry and by dyed and undyed rosemary extracts in tewametry. The sequential treatment (postirritation) once a day for 5 days was without any effect. Thus, a protective effect of some marigold and rosemary extracts against ICD could be shown in the elicitation phase. Copyright (c) 2005 S. Karger AG, Basel.

  8. Concepts in radiation protection

    International Nuclear Information System (INIS)

    Oncescu, M.

    1996-01-01

    This monograph provides basic notions and principles in dosimetry and radiation protection in compliance with two fundamental works: IAEA Safety Series No.115 - International Basic Safety Standards for Protection against Ionizing Radiation and for the Safety of Radiation Sources - and Publication no. 60 of International Commission on Radiological Protection. After the review of quantities and units necessary in radiation protection, the book presents the new values of dose limits as well as the values of 'radiation weighting factor', 'tissue weighting factor' and 'conversion factor intake-dose' (committed effective dose per unit intake) by ingestion and inhalation for 30 most important radionuclides. The new values of dose limits, lower than the old values, are a challenge for the radiation protection, especially of the 'public' where the dose limit diminished by a factor of five relative to the earlier edition. The new value of dose limit for public, 1 mSv per year (obviously over the natural exposure of 2.4 mSv per year), imposes new action ways and levels in radiation protection, especially in some cases of exacerbated natural radioactivity. The book provides the calculus of external exposure with the Gamma constant expressed in adequate units, to make the calculation easier. In the calculus of protection shield for gamma sources one uses a method, which while approximate helps save time. The calculus of internal exposure is made using the conversion factor intake-dose. Finally, the 'dosimetric watch' of the natural and artificial radioactivity of the atmosphere, hydrosphere and biosphere is intended to comply with the International Basic Safety Standards. Each chapter ends with a set of illustrative problems which enhances the reader's understanding of underlying concepts and current methods used in the field

  9. ETHIOPIAN WITNESS PROTECTION SYSTEM: COMPARATIVE ...

    African Journals Online (AJOL)

    witness protection involves social cohesion of the protected person. Scholars ..... interests' need to balance an accused right to know and confront prosecution ..... was reduced to 2.5 years through the fast tracking of witness protection cases.

  10. Radiation protection optimization of workers

    International Nuclear Information System (INIS)

    Lochard, J.

    1994-11-01

    This report presents the contribution of CEPN (study center on protection evaluation in nuclear area) to the Days of the French Radiation Protection Society (SFRP) on optimization of workers radiation protection in electronuclear, industrial and medical areas

  11. Anticorrosion protection of uranium

    Energy Technology Data Exchange (ETDEWEB)

    Goncharov, Ivan D.; Kazakovskaya, Tatiana; Tukmakov, Victor; Shapovalov, Vyacheslav [Russian Federal Nuclear Center-VNIIEF, 37, Mira Ave., RU-607190 Sarov (Nizhnii Gorod), 010450 (Russian Federation)

    2004-07-01

    Uranium in atmospheric conditions is non-stable. Sloughing products are being generated on its surface during storage or use. These corrosion products make many difficulties because of necessity to provide personnel safety. Besides, uranium corrosion may cause damage in parts. The first works devoted to uranium corrosion were performed in the framework of the USA Manhattan Project in the early forties of last century. Various methods of uranium protection were investigated, among them the galvanic one was the most studied. Later on the galvanic technology was patented. The works on this problem remains urgent up to the present time. In Russia, many methods of uranium corrosion protection, mainly against atmospheric corrosion, were tried on. In particular, such methods as diffusion zinc and paint coating were investigated. In the first case, a complex intermetallic U-Zn compound was formed but its protection was not reliable enough, this protection system was inconvenient and uncertain and that is why an additional paint coating was necessary. In the case of paint coatings another problem appeared. It was necessary to find such a coating where gas-permeability would prevail over water-permeability. Otherwise significant uranium corrosion occurs. This circumstance together with low mechanical resistance of paint coatings does not allow to use paint coating for long-term protection of uranium. Currently, there are following methods of uranium protection: ion-plasma, galvanic and thermo-vacuum annealing. These are described in this paper. In the end the issue of corrosion protection in reactor core zones is addressed. Here the greatest difficulties are caused when enriched uranium heated up to 500 deg. C needs anticorrosion protection. In this case various metal coatings are not reliable because of brittle inter-metallide formation. The reliable protection may be provided only up to the temperature plus 400 - 500 deg. C with the help of galvanic copper coating since

  12. Anticorrosion protection of uranium

    International Nuclear Information System (INIS)

    Goncharov, Ivan D.; Kazakovskaya, Tatiana; Tukmakov, Victor; Shapovalov, Vyacheslav

    2004-01-01

    Uranium in atmospheric conditions is non-stable. Sloughing products are being generated on its surface during storage or use. These corrosion products make many difficulties because of necessity to provide personnel safety. Besides, uranium corrosion may cause damage in parts. The first works devoted to uranium corrosion were performed in the framework of the USA Manhattan Project in the early forties of last century. Various methods of uranium protection were investigated, among them the galvanic one was the most studied. Later on the galvanic technology was patented. The works on this problem remains urgent up to the present time. In Russia, many methods of uranium corrosion protection, mainly against atmospheric corrosion, were tried on. In particular, such methods as diffusion zinc and paint coating were investigated. In the first case, a complex intermetallic U-Zn compound was formed but its protection was not reliable enough, this protection system was inconvenient and uncertain and that is why an additional paint coating was necessary. In the case of paint coatings another problem appeared. It was necessary to find such a coating where gas-permeability would prevail over water-permeability. Otherwise significant uranium corrosion occurs. This circumstance together with low mechanical resistance of paint coatings does not allow to use paint coating for long-term protection of uranium. Currently, there are following methods of uranium protection: ion-plasma, galvanic and thermo-vacuum annealing. These are described in this paper. In the end the issue of corrosion protection in reactor core zones is addressed. Here the greatest difficulties are caused when enriched uranium heated up to 500 deg. C needs anticorrosion protection. In this case various metal coatings are not reliable because of brittle inter-metallide formation. The reliable protection may be provided only up to the temperature plus 400 - 500 deg. C with the help of galvanic copper coating since

  13. Special protective concretes

    International Nuclear Information System (INIS)

    Bouniol, P.

    2001-01-01

    Concrete is the most convenient material when large-scale radiation protection is needed. Thus, special concretes for nuclear purposes are used in various facilities like reactors, reprocessing centers, storage sites, accelerators, hospitals with nuclear medicine equipment, food ionization centers etc.. The recent advances made in civil engineering for the improvement of concrete durability and compactness are for a large part transposable to protection concretes. This article presents the basic knowledge about protection concretes with the associated typological and technological aspects. A large part is devoted to the intrinsic properties of concretes and to their behaviour in irradiation and temperature conditions: 1 - definition and field of application of special protective concretes; 2 - evolution of concepts and technologies (durability of structures, techniques of formulation, new additives, market evolution); 3 - design of protective structures (preliminary study, radiation characteristics, thermal constraints, damping and dimensioning, mechanical criteria); 4 - formulation of special concretes (general principles, granulates, hydraulic binders, pulverulent additives, water/cement ratio, reference composition of some special concretes); 5 - properties of special concretes (damping and thermo-mechanical properties); 6 - induced-irradiation and temperature phenomena (activation, radiolysis, mineralogical transformations, drying, shrinking, creep, corrosion of reinforcement). (J.S.)

  14. Digital integrated protection system

    International Nuclear Information System (INIS)

    Savornin, M.; Furet, M.

    1978-01-01

    As a result of technological progress it is now possible to achieve more elaborate protection functions able to follow more closely the phenomena to be supervised. For this reason the CEA, Framatome and Merlin/Gerin/CERCI have undertaken in commonn to develop a Digital Integrated Protection System (D.I.P.S.). This system is designed with the following aims: to improve the safety of the station, . to improve its availability, . to facilitate installation, . to facilitate tests and maintenance. The main characteristics adopted are: . possibilities of obtaining more elaborate monitoring and protection algorithm treatments, . order 4 redundancy of transducers, associated instruments and signal processing, . possibility of inhibiting part of the protection system, . standardisation of equipment, physical and electrical separation of redundant units, . use of multiplexed connections, . automation of tests. Four flow charts are presented: - DIPS with four APUP (Acquisition and Processing Unit for Protection) - APUP - LSU (Logic Safeguard Unit), number LSU corresponding to number fluidic safeguard circuits, - structure of a function unit, - main functions of the APUP [fr

  15. System level ESD protection

    CERN Document Server

    Vashchenko, Vladislav

    2014-01-01

    This book addresses key aspects of analog integrated circuits and systems design related to system level electrostatic discharge (ESD) protection.  It is an invaluable reference for anyone developing systems-on-chip (SoC) and systems-on-package (SoP), integrated with system-level ESD protection. The book focuses on both the design of semiconductor integrated circuit (IC) components with embedded, on-chip system level protection and IC-system co-design. The readers will be enabled to bring the system level ESD protection solutions to the level of integrated circuits, thereby reducing or completely eliminating the need for additional, discrete components on the printed circuit board (PCB) and meeting system-level ESD requirements. The authors take a systematic approach, based on IC-system ESD protection co-design. A detailed description of the available IC-level ESD testing methods is provided, together with a discussion of the correlation between IC-level and system-level ESD testing methods. The IC-level ESD...

  16. Sports eyewear protective standards.

    Science.gov (United States)

    Dain, Stephen J

    2016-01-01

    Eye injuries sustained during sport comprise up to 20 per cent of all injuries to the eye serious enough for medical attention to be sought. The prevalence of eye injuries in sport is not easily assessed due to lack of authoritative participation rates, so most studies report total numbers in a time period. The evidence on the proportion of all ocular injuries that are from sport is reviewed. The relative frequencies in different sports are compared in a qualitative manner and the sports with greater numbers of ocular injuries are detailed. In common with occupational injuries to the eye, most sports eye injuries are considered preventable. The hierarchy of action for occupational risk is detailed and adapted to use in a sports scenario. All the available international, regional and national standards on sports eye protection are detailed and their provisions compared. The major function of the standards is to provide adequate protection against the hazard of the sport concerned. These are detailed and compared as a function of energy transfer. Eye protection must not introduce additional or secondary hazards (for instance, fracturing into sharp fragments on impact) and not introduce features that would deter the wearing of eye protection (for instance, restricting field of view to impede playing the sport). The provisions of the standards intended to limit secondary hazards are detailed and compared. The need for future work in standards writing and the activities of the International Standardization Organization in sports eye protection are detailed. © 2016 Optometry Australia.

  17. Instructed officers Radiation Protection

    International Nuclear Information System (INIS)

    2007-01-01

    This law contains instructions on the prevention of radiological and contains 4 articles Article I: describe the responsibilities of the institutions that operate within the scope of radiological protection in terms of the number of radiation protection officers and personal Supervisors who available in the practices radiation field. Article II: talking about the conditions of radiation protection officers that must be available in the main officers and working field in larg institutions and thecondition of specific requirements for large enterprises of work permits in the field of radiological work that issued by the Council. Article III: the functions and duties of officers in the prevention of radiological oversee the development of radiation protection programmes in the planning stages, construction and preparing the rules of local labour and what it lead of such tasks.Article IV: radiation protection officers powers: to modify and approve the programme of prevention and radiation safety at the company, stop any unsafe steps, amend the steps of the usage, operation of materials, devices and so on

  18. Characteristics of protective instrumentation

    International Nuclear Information System (INIS)

    Reichart, G.

    1982-01-01

    Protective Instrumentation (PI) for Nuclear Power Plants (NPP) is a general term for an highly reliable instrumentation, which provides information for keeping the system within safe limits, for initation of countermeasures in the case of an incident or for mitigation of consequences of an accident. In German NPPs one can find a hierarchical structure of protective instrumentation, wherein the Reactor Protection System (RPS) has the highest priority. To meet the reliability requirements different design principles are used, like - redundancy - diversity - fail safe - decoupling. The presentation gives an overview about the different design principles and characterizes their reliability aspects. As an example for the technical realization the RPS of a German NPP is discussed in some detail. Furthermore some information about other type of PI is given and reliability aspects of the interaction of operating personell with these systems are mentioned. (orig.)

  19. Radiation protecting clothing materials

    International Nuclear Information System (INIS)

    Mio, Kotaro; Ijiri, Yasuo.

    1986-01-01

    Purpose: To provide radiation protecting clothing materials excellent in mechanical strength, corrosion resistance, flexibility and flexing strength. Constitution: The radiation protecting clothing materials according to this invention has pure lead sheets comprising a thin pure lead foil of 50 to 150 μm and radiation resistant organic materials, for example, polyethylene with high neutron shielding effect disposed to one or both surfaces thereof. The material are excellent in the repeating bending fatigue and mechanical strength, corrosion resistance and flexibility and, accordingly, radiation protecting clothings prepared by using them along or laminating them also possess these excellent characteristics. Further, they are excellent in the handlability, particularly, durability to the repeated holding and extension, as well as are preferable in the physical movability and feeling upon putting. The clothing materials may be cut into an appropriate size, or stitched into clothings made by radiation-resistant materials. In this case, pure lead sheets are used in lamination. (Horiuchi, T.)

  20. Reactor protection system

    International Nuclear Information System (INIS)

    Fairbrother, D.B.; Lesniak, L.M.; Orgera, E.G.

    1977-10-01

    The report describes the reactor protection system (RPS-II) designed for use on Babcock and Wilcox 145-, later 177-, and 205-fuel assembly pressurized water reactors. In this system, relays in the trip logic have been replaced by solid state devices. A calculating module for the low DNBR, pump status, and offset trip functions has replaced the overpower trip (based on flow and imbalance), the power/RC pump trip, and the variable low-pressure trip. Included is a description of the changes from the present Oconee-type reactor protection system (RPS-I), a functional and hardware description of the calculating module, a description of the software programmed in the calculating module, and a discussion of the qualification program conducted to ensure that the degree of protection provided by RPS-II is not less than that provided by previously licensed systems supplied by B and W

  1. Protective and preventative measures

    International Nuclear Information System (INIS)

    Freedman, P. N

    2002-01-01

    Health care workers who come in contact with blood and other body fluids in their working environment risk being exposed to blood borne diseases such as human immunodeficiency virus (HIV), hepatitis B and C. An anti-hepatitis B vaccine is available as well as hepatitis B immunoglobulin but no vaccine is available against hepatitis C and HIV. The best way to protect against exposure to blood and body fluids is to use 'Universal Precautions' which encourage safe working methods. If an exposure does take place it should be regarded as an urgent medical problem and every facility should have a management policy to deal with this problem. The source patient's rights must also be protected. The preventative and protective measures available to health care workers as well as practical suggestions to carry out in the event of an exposure are discussed (Au)

  2. Smart Machine Protection System

    International Nuclear Information System (INIS)

    Clark, S.; Nelson, D.; Grillo, A.; Spencer, N.; Hutchinson, D.; Olsen, J.; Millsom, D.; White, G.; Gromme, T.; Allison, S.; Underwood, K.; Zelazny, M.; Kang, H.

    1991-11-01

    A Machine Protection System implemented on the SLC automatically controls the beam repetition rates in the accelerator so that radiation or temperature faults slow the repetition rate to bring the fault within tolerance without shutting down the machine. This process allows the accelerator to aid in the fault diagnostic process, and the protection system automatically restores the beams back to normal rates when the fault is diagnosed and corrected. The user interface includes facilities to monitor the performance of the system, and track rate limits, faults, and recoveries. There is an edit facility to define the devices to be included in the protection system, along with their set points, limits, and trip points. This set point and limit data is downloaded into the CAMAC modules, and the configuration data is compiled into a logical decision tree for the 68030 processor. 3 figs

  3. Advanced Worker Protection System

    International Nuclear Information System (INIS)

    1996-04-01

    The Advanced Worker Protection System (AWPS) is a liquid-air-based, self-contained breathing and cooling system with a duration of 2 hrs. AWPS employs a patented system developed by Oceaneering Space Systems (OSS), and was demonstrated at their facility in Houston, TX as well as at Kansas State University, Manhattan. The heart of the system is the life-support backpack that uses liquid air to provide cooling as well as breathing gas to the worker. The backpack is combined with advanced protective garments, an advanced liquid cooling garment (LCG), a respirator, and communications and support equipment. The prototype unit development and testing under Phase 1 has demonstrated that AWPS has the ability to meet performance criteria. These criteria were developed with an understanding of both the AWPS capabilities and the DOE decontamination and decommissioning (D and D) activities protection needs

  4. The protection of possession

    Directory of Open Access Journals (Sweden)

    Popov Danica

    2011-01-01

    Full Text Available Protection in disputes for the protection of possession can be attained through the following actions a for dispossession (interdictum recuperande possessionis and b with an action for the disturbance of possession (interdictum retinendae possessionis. The general feature of these disputes is that there is only discussion on the facts and not a legal matters. Subject matter jurisdiction for the resolution of such disputes belongs to the court of general jurisdiction, while the dispute itself is a litigation. The special rule of proceedings of action for disturbance are: provisionality of the protection of possession; urgency in proceedings; initiation of the proceedings; limiting of objection; prescribing temporary measures; rendering a ruling in the form of order; appeals which may be filed within a short deadline and which does not have suspensive effect (do not delay the execution of the order; revision is not allowed etc.

  5. Preventive Radiation Protection Act

    International Nuclear Information System (INIS)

    Roewer, H.

    1988-01-01

    The commentary is intended to contribute to protection of the population by a practice-oriented discussion and explanation of questions arising in connection with the Preventive Radiation Protection Act. Leaving aside discussions about abandonment of nuclear power, or criticism from any legal point of view, the commentary adopts the practical approach that accepts, and tries to help implementing, the act as it is. It is a guide for readers who are not experts in the law and gives a line of orientation by means of explanations and sometimes by citations from other acts (in footnotes). The commentary also presents the EURATOM Directive No. 3954/87 dated 22 December 1987, the EC Directive No. 3955/87 dated 22 December 1987, and the EC Directive No. 1983/88 dated 5 July 1988. A tabular survey shows the system of duties and competences defined by the Preventive Radiation Protection Act. (RST) [de

  6. Education in Radiation Protection

    International Nuclear Information System (INIS)

    Dodig, D.; Kasal, B.; Tezak, S.; Poropat, M.; Kubelka, D.

    2001-01-01

    Full text: This paper discussed the problem of the education in radiation protection. All aspects of education are included started with primary school and lasted with very specialised courses for the experts. In the last few years the lack of interest for education in radiation protection was recognised by many agencies included also IAEA and EU commission. In this paper the reasons for this situation will be presented and the way how to promote this subject again. It is not possible to prevent effects of radiation on environment and population if qualified and well educated experts do not exist. The situation in the field of education in radiation protection in Croatia will be presented, according to the new regulations in this field. (author)

  7. Smart machine protection system

    International Nuclear Information System (INIS)

    Clark, S.; Nelson, D.; Grillo, A.

    1992-01-01

    A Machine Protection System implemented on the SLC automatically controls the beam repetition rates in the accelerator so that radiation or temperature faults slow the repetition rate to bring the fault within tolerance without shutting down the machine. This process allows the accelerators to aid in the fault diagnostic process, and the protection system automatically restores the beams back to normal rates when the fault is diagnosed and corrected. The user interface includes facilities to monitor the performance of the system, and track rate limits, faults, and recoveries. There is an edit facility to define the devices to be included in the protection system, along with their set points, limits, and trip points. This set point and limit data is downloaded into the CAMAC modules, and the configuration data is complied into a logical decision tree for the 68030 processor. (author)

  8. New radiation protection law

    International Nuclear Information System (INIS)

    1985-01-01

    The structure of the existing legislation and its contents and aims are reconsidered. New rules which correspond to the present situation are to be established. Also the fundamental principles of the task and methods of radiation protection are to be changed. The main effort will be to create conditions so that all human beings will be protected against the harmful effects of radiation. The effects on plants, animals and on the environment should be considered as well. The legislation should include both ionizing and non-ionizing radiation. The main responsibility of protection should stay with the central authority. Licensing of apparatus, liability for medical applications and radioactive waste is discussed. Granting of permissions and control should be accomplished by the authority. Cooperation with other national and international authorities is dealt with. (G.B.)

  9. Radiation protection - Revision of French radiation protection regulations (1988)

    International Nuclear Information System (INIS)

    Mayoux, J.C.

    1989-01-01

    This article analyses the recent amendments to the 1966 and 1975 Decrees on general radiation protection principles and radiation protection of workers in large nuclear installations respectively and also describes national radiation protection law. In particular, the amendments incorporate the revised EURATOM basic radiation protection standards and the new international units (sievert and becquerel replace rem and curie) in the Decrees. (NEA) [fr

  10. Radiation protection in the field of environmental protection

    International Nuclear Information System (INIS)

    Zhao Yamin

    2003-01-01

    The relationship of radiation protection with environmental protection, the sources that may give rise to the environmental radiation contamination, and the system of radiation protection and the fundamental principles and requirements for radiation environmental management are introduced. Some special radiation protection problems faced with in the radiation environmental management are discussed. (author)

  11. Radiation protection in hospitals

    International Nuclear Information System (INIS)

    MOuld, R.F.

    1985-01-01

    A book on radiation protection in hospitals has been written to cater for readers with different backgrounds, training and needs by providing an elementary radiation physics text in Part I and an advanced, comprehensive Part II relating to specific medical applications of X-rays and of radioactivity. Part I includes information on basic radiation physics, radiation risk, radiation absorption and attenuation, radiation measurement, radiation shielding and classification of radiation workers. Part II includes information on radiation protection in external beam radiotherapy, interstitial source radiotherapy, intracavitary radiotherapy, radioactive iodine-131 radiotherapy, nuclear medicine diagnostics and diagnostic radiology. (U.K.)

  12. Radiation protection in space

    Energy Technology Data Exchange (ETDEWEB)

    Blakely, E.A. [Lawrence Berkeley Lab., CA (United States); Fry, R.J.M. [Oak Ridge National Lab., TN (United States)

    1995-02-01

    The challenge for planning radiation protection in space is to estimate the risk of events of low probability after low levels of irradiation. This work has revealed many gaps in the present state of knowledge that require further study. Despite investigations of several irradiated populations, the atomic-bomb survivors remain the primary basis for estimating the risk of ionizing radiation. Compared to previous estimates, two new independent evaluations of available information indicate a significantly greater risk of stochastic effects of radiation (cancer and genetic effects) by about a factor of three for radiation workers. This paper presents a brief historical perspective of the international effort to assure radiation protection in space.

  13. Protection from space radiation

    International Nuclear Information System (INIS)

    Tripathi, R.K.; Wilson, J.W.; Shinn, J.L.

    2000-01-01

    The exposures anticipated for astronauts in the anticipated human exploration and development of space will be significantly higher (both annual and carrier) than for any other occupational group. In addition, the exposures in deep space result largely from galactic cosmic rays for which there is as yet little experience. Some evidence exists indicating that conventional linear energy transfer defined protection quantities (quality factors) may not be appropriate. The authors evaluate their current understanding of radiation protection with laboratory and flight experimental data and discuss recent improvements in interaction models and transport methods

  14. Commercial Aircraft Protection

    Energy Technology Data Exchange (ETDEWEB)

    Ehst, David A. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2016-10-26

    This report summarizes the results of theoretical research performed during 3 years of P371 Project implementation. In results of such research a new scientific conceptual technology of quasi-passive individual infrared protection of heat-generating objects – Spatial Displacement of Thermal Image (SDTI technology) was developed. Theoretical substantiation and description of working processes of civil aircraft individual IR-protection system were conducted. The mathematical models and methodology were presented, there were obtained the analytical dependencies which allow performing theoretical research of the affect of intentionally arranged dynamic field of the artificial thermal interferences with variable contrast onto main parameters of optic-electronic tracking and homing systems.

  15. Radiation Protection Dosimetry

    International Nuclear Information System (INIS)

    Kramer, H.M.; Schnuer, K.

    1992-01-01

    The contributions presented during the seminar provided clear evidence that radiation protection of the patient plays an increasingly important role for manufacturers of radiological equipment and for regulatory bodies, as well as for radiologists, doctors and assistants. The proceedings of this seminar reflect the activities and work in the field of radiation protection of the patient and initiate further action in order to harmonize dosimetric measurements and calculations, to ameliorate education and training, to improve the technical standards of the equipment and to give a push to a more effective use of ionising radiation in the medical sector

  16. Environmental protection implementation plan

    International Nuclear Information System (INIS)

    Holland, R.C.

    1998-03-01

    This Environmental Protection Implementation Plan is intended to ensure that the environmental program objectives of Department of Energy Order 5400.1 are achieved at SNL/California. This document states SNL/California's commitment to conduct its operations in an environmentally safe and responsible manner. The Environmental Protection Implementation Plan helps management and staff comply with applicable environmental responsibilities. SNL is committed to operating in full compliance with the letter and spirit of applicable environmental laws, regulations, and standards. Furthermore, SNL/California strives to go beyond compliance with legal requirements by making every effort practical to reduce impacts to the environment to levels as low as reasonably achievable

  17. Material for radioactive protection

    Science.gov (United States)

    Taylor, R.S.; Boyer, N.W.

    A boron containing burn resistant, low-level radiation protection material useful, for example, as a liner for radioactive waste disposal and storage, a component for neutron absorber, and a shield for a neutron source is described. The material is basically composed of borax in the range of 25 to 50%, coal tar in the range of 25 to 37.5%, with the remainder being an epoxy resin mix. A preferred composition is 50% borax, 25% coal tar and 25% epoxy resin. The material is not susceptible to burning and is about 1/5 the cost of existing radiation protection material utilized in similar applications.

  18. Protecting patron privacy

    CERN Document Server

    Beckstrom, Matthew

    2015-01-01

    In a world where almost anyone with computer savvy can hack, track, and record the online activities of others, your library can serve as a protected haven for your visitors who rely on the Internet to conduct research-if you take the necessary steps to safeguard their privacy. This book shows you how to protect patrons' privacy while using the technology that your library provides, including public computers, Internet access, wireless networks, and other devices. Logically organized into two major sections, the first part of the book discusses why the privacy of your users is of paramount

  19. Radiation protection in space

    International Nuclear Information System (INIS)

    Blakely, E.A.; Fry, R.J.M.

    1995-01-01

    The challenge for planning radiation protection in space is to estimate the risk of events of low probability after low levels of irradiation. This work has revealed many gaps in the present state of knowledge that require further study. Despite investigations of several irradiated populations, the atomic-bomb survivors remain the primary basis for estimating the risk of ionizing radiation. Compared to previous estimates, two new independent evaluations of available information indicate a significantly greater risk of stochastic effects of radiation (cancer and genetic effects) by about a factor of three for radiation workers. This paper presents a brief historical perspective of the international effort to assure radiation protection in space

  20. Radiation protection in education

    International Nuclear Information System (INIS)

    Viragh, Elemer

    1985-01-01

    The education of secondary school students in the fields of nuclear sciences was strictly limited according to the 9th recommendations of the ICRP issued in 1966 saying that people under age 18 are not allowed to deal with ionizing radiations. Due to the changes concerning the concept of radiation protection, new opportunities for teaching nuclear technology even in the secondary schools were opened. The 36th recommendations of the ICRP published in 1983 dealing with the maximum permissible doses and the measures taken for radiation protection should be kept in mind while organizing the education of the pupils between age 16 and 18. (V.N.)

  1. Radiation Protection Proclamation

    International Nuclear Information System (INIS)

    1993-01-01

    A proclamation of the Government of Ethiopia, cited as the radiation protection proclamation number 79/1993 was prepared with the objective to establish a national radiation protection authority that formulates policies, controls and supervises activities involving all sources of radiation and lay down laws governing such activities in order to ensure public safety against associated hazards while allowing radiation related activities to be carried out for the benefit of the public . The Authority is guided by an inter-ministerial board and is accountable to the Ethiopian Science and Technology Commission

  2. Radiation protection glossary

    International Nuclear Information System (INIS)

    1986-01-01

    The glossary is intended to be used as a terminology standard for IAEA documentation on radiation protection. An effort has been made to use definitions contained in internationally accepted publications such as recommendations of the International Commission on Radiological Protection (ICRP), standards of the International Organization for Standardization (ISO) and of the International Electrotechnical Commission (IEC), reports of the International Commission on Radiation Units and Measurements (ICRU), with only slight modifications in order to tailor them more closely to IAEA needs. The glossary is restricted to ionizing radiation

  3. Programme Biology - Health protection

    International Nuclear Information System (INIS)

    1975-01-01

    The scientific results for 1975, of the five-year Biology-Health Protection programme adopted in 1971, are presented in two volumes. In volume one, Research in Radiation Protection are developed exclusively, including the following topics: measurement and interpretation of radiation (dosimetry); transfer of radioactive nuclides in the constituents of the environment; hereditary effects of radiation; short-term effects (acute irradiation syndrome and its treatment); long-term effects and toxicology of radioactive elements. In volume, two Research on applications in Agriculture and Medicine are developed. It includes: mutagenesis; soil-plant relations; radiation analysis; food conservation; cell culture; radioentomology. Research on applications in Medicine include: Nuclear Medicine and Neutron Dosimetry

  4. Painting for protection

    International Nuclear Information System (INIS)

    Stepto, G.G.C.

    1981-01-01

    Despite the use of special alloys, paint is still the most economical and practical method of protecting steel. Chlorinated rubber resin based paint systems are shown to be satisfactory in areas requiring decontamination as well as for outside exposed areas of nuclear power plants. (author)

  5. Radiation protection glossary

    International Nuclear Information System (INIS)

    Othman, Ibrahim; Abdul-Rahim, Maha

    1989-12-01

    This glossary contains the arabic equivalent of all the terms included in the IAEA Safety Series No.76 (which is a selected basic terms used in IAEA publications), thus this glossary contains English, French, Spanish, Russian, and Arabic. It is intended to facilitate the work of arabic speaking scientists involved in the field of radiation protection

  6. Radiation protection to firemen

    International Nuclear Information System (INIS)

    Almeida, E.S. de.

    1985-01-01

    The basic Knowledge about ionizing radiation oriented for firemen, are presented. The mainly damage and effects caused by radiation exposure as well as the method of radiation protection are described in simple words. The action to be taken in case of fire involving radiation such as vehicles transporting radioactive materials are emphasized. (author)

  7. Marine Mammal Protection Act

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Marine Mammal Protection Act (MMPA or Act) prohibits, with certain exceptions, the "take" of marine mammals in U.S. waters and by U.S. citizens on the high seas,...

  8. Depth protection system

    International Nuclear Information System (INIS)

    Arita, Setsuo; Izumi, Shigeru; Suzuki, Satoru; Noguchi, Atomi.

    1988-01-01

    Purpose: To previously set a nuclear reactor toward safety side by the reactor scram if an emergency core cooling system is failed to operate. Constitution If abnormality occurs in an emergency core cooling system or an aqueous boric acid injection system, a reactor protection system is operated and, if the reactor protection system shows an abnormal state, a control rod withdrawal inhibition system is operated as a fundamental way. For instance, when the driving power source voltage for the emergency core cooling system is detected and, if it is lower than a predetermined value, the reactor protection system is operated. Alternatively, if the voltage goes lower than the predetermined value, the control rod withdrawal is inhibited. In addition, stopping for the feedwater system is inhibited. Further, integrity of the driving means for the emergency core cooling system is positively checked and the protection function is operated depending on the result of check. Since the nuclear reactor can be set toward the safety side even if the voltage for the driving power source of the aqueous boric acid injection system is lower than a predetermined value, the reactor safety can further be improved. (Horiuchi, T.)

  9. Radiation Protection in Guatemala

    International Nuclear Information System (INIS)

    Carazo, N.

    1979-01-01

    The tasks connected with radiation protection are allocated to the National Institute for Nuclear Energy in Guatemala. Regulatory measures are further needed to identify the responsibilities of various authorities to ensure that all radiation workers are provided with personal dosemeters. (author)

  10. Protectiveness, Persecution, and Powerlessness

    Science.gov (United States)

    Weeks, Kathleen G.

    1977-01-01

    This paper is an investigation of family pathology from a transactional viewpoint. The thesis proposed is that family pathology is a means of protecting the family and ensuring its survival. The father, mother, and child assume and exchange the roles of the Karpman drama triangle; namely, Persecutor, Victim, and Rescuer. (Author)

  11. Beyond Pain and Protection

    DEFF Research Database (Denmark)

    Lee, Jieun

    2007-01-01

    discourse on homosexual youth in Korea, protection discourse and pain discourse, the notion of identity itself will be critically examined and the girls' agency in destabilizing heteronormativity will be discussed. This study also deals with the appropriation of popular culture by the girls, suggesting...

  12. Applied Crop Protection 2017

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup; Nielsen, Bent Jørgen; Mathiassen, Solvejg Kopp

    Linket til højre henviser til rapporten i trykt format til download. This publication contains results from crop protection trials which were carried out at the Department of Agroecology within the area of gricultural crops. Most of the results come from field trials, but results from greenhouse...

  13. Maternity Protection at Work.

    Science.gov (United States)

    World of Work, 1998

    1998-01-01

    Discusses the need for maternity benefits for working women. Suggests that although most countries provide paid maternity leave by law, there is a gap between that law and practice. Includes a chart depicting maternity protection (length of leave, cash benefits, who pays) around the world. (JOW)

  14. Radiation protection in Switzerland

    International Nuclear Information System (INIS)

    Brunner, H.

    1990-01-01

    Switzerland's present radiation protection regulations are based on only two paragraphs of the atomic law but have been very successful in practice. A new radiation protection law, separated from nuclear legislation and valid for all application of ionizing radiation and radioctive materials, was proposed and drafted by the Federal Commission on Radiation Protection and has now been accepted by parliament with only minor modifications. The draft of the revised regulations which also will cover all applications, should be ready for consultations next year. Both the law (which contains principles but no figures such as limits) and the regulations incorporate the latest state of ICRP recommendations and are formulated in such a way as to allow application of or quick adaptation to the new basic ICRP recommendation expected for 1991. The legislation is flexible, with a relatively low regulation density and leaves sufficient room for professional judgement on a case by case basis both for authorities and for the specialists responsible for radiation protection in practice. (orig./HSCH)

  15. Inflation Protected Investment Strategies

    Directory of Open Access Journals (Sweden)

    Mirco Mahlstedt

    2016-03-01

    Full Text Available In this paper, a dynamic inflation-protected investment strategy is presented, which is based on traditional asset classes and Markov-switching models. Different stock market, as well as inflation regimes are identified, and within those regimes, the inflation hedging potential of stocks, bonds, real estate, commodities and gold are investigated. Within each regime, we determine optimal investment portfolios driven by the investment idea of protection from losses due to changing inflation if inflation is rising or high, but decoupling the performance from inflation if inflation is low. The results clearly indicate that these asset classes behave differently in different stock market and inflation regimes. Whereas in the long-run, we agree with the general opinion in the literature that stocks and bonds are a suitable hedge against inflation, we observe for short time horizons that the hedging potential of each asset class, especially of real estate and commodities, depend strongly on the state of the current market environment. Thus, our approach provides a possible explanation for different statements in the literature regarding the inflation hedging properties of these asset classes. A dynamic inflation-protected investment strategy is developed, which combines inflation protection and upside potential. This strategy outperforms standard buy-and-hold strategies, as well as the well-known 1 N -portfolio.

  16. Applied Crop Protection 2017

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup; Nielsen, Bent Jørgen; Mathiassen, Solvejg Kopp

    Linket til højre henviser til rapporten i trykt format til download. This publication contains results from crop protection trials which were carried out at the Department of Agroecology within the area of gricultural crops. Most of the results come from field trials, but results from greenhouse ...

  17. Applied crop protection 2016

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup; Nielsen, Bent Jørgen; Jensen, Peter Kryger

    This publication contains results from crop protection trials which were carried out at the Department of Agroecology within the area of agricultural crops. Most of the results come from field trials, but results from greenhouse and semi-field trials are also included. The report contains results...

  18. Consumer Protection for Educators.

    Science.gov (United States)

    Wallace, James M.

    Educational changes are examined from the perspective of consumer protection--the direct consumers are the teachers being prepared; the indirect consumers are the students and the society that supports the schools. During the colonial and early national periods of American history, there was an absence of formal and separate teacher education.…

  19. Armored garment for protecting

    Science.gov (United States)

    Purvis, James W [Albuquerque, NM; Jones, II, Jack F.; Whinery, Larry D [Albuquerque, NM; Brazfield, Richard [Albuquerque, NM; Lawrie, Catherine [Tijeras, NM; Lawrie, David [Tijeras, NM; Preece, Dale S [Watkins, CO

    2009-08-11

    A lightweight, armored protective garment for protecting an arm or leg from blast superheated gases, blast overpressure shock, shrapnel, and spall from a explosive device, such as a Rocket Propelled Grenade (RPG) or a roadside Improvised Explosive Device (IED). The garment has a ballistic sleeve made of a ballistic fabric, such as an aramid fiber (e.g., KEVLAR.RTM.) cloth, that prevents thermal burns from the blast superheated gases, while providing some protection from fragments. Additionally, the garment has two or more rigid armor inserts that cover the upper and lower arm and protect against high-velocity projectiles, shrapnel and spall. The rigid inserts can be made of multiple plies of a carbon/epoxy composite laminate. The combination of 6 layers of KEVLAR.RTM. fabric and 28 plies of carbon/epoxy laminate inserts (with the inserts being sandwiched in-between the KEVLAR.RTM. layers), can meet the level IIIA fragmentation minimum V.sub.50 requirements for the US Interceptor Outer Tactical Vest.

  20. Environmental radiation protection standards

    International Nuclear Information System (INIS)

    Richings, L.D.G.; Morley, F.; Kelley, G.N.

    1978-04-01

    The principles involved in the setting of radiological protection standards are reviewed, and the differences in procedures used by various countries in implementing them are outlined. Standards are taken here to mean the specific numerical limits relating to radiation doses to people or to amounts of radioactive material released into the environment. (author)

  1. Solar collector overheating protection

    NARCIS (Netherlands)

    Slaman, M.J.; Griessen, R.P.

    Prismatic structures in a thermal solar collector are used as overheating protection. Such structures reflect incoming light efficiently back whenever less thermal power is extracted from the solar collector. Maximum thermal power is generated when the prismatic structure is surrounded by a

  2. Radiation protection textbook

    International Nuclear Information System (INIS)

    Gambini, D.J.; Granier, R.

    2007-01-01

    This textbook of radiation protection presents the scientific bases, legal and statutory measures and technical means of implementation of the radioprotection in the medical and industrial sectors, research and nuclear installations. It collects the practical information (organization, analysis of post, prevention, evaluation and risks management, the controls, the training and the information) usually scattered and the theoretical knowledge allowing every person using ionizing radiation: To analyze jobs in controlled areas, to watch the respect for the current regulations, to participate in the training and in the information of the staffs exposed to intervene in accidental situation. This third edition is widely updated and enriched by the most recent scientific and legal data concerning, notably, the human exposure, the dosimetry, the optimization of the radiation protection and the epidemiological inquiries. The contents is as follows: physics of ionizing radiation, ionizing radiation: origin and interaction with matter, dosimetry and protection against ionizing radiation, detection and measurement of ionizing radiation, radiobiology, legal measures relative to radiation protection, human exposure of natural origin, human exposure of artificial origin, medical, dental and veterinarian radiology, radiotherapy, utilization of unsealed sources in medicine and research, electronuclear industry, non nuclear industrial and aeronautical activities exposing to ionizing radiation, accidental exposures. (N.C.)

  3. Towards data protection compliance

    NARCIS (Netherlands)

    Zannone, N.; Petkovic, M.; Etalle, S.

    2010-01-01

    Privacy and data protection are fundamental issues nowadays for every organization. This paper calls for the development of methods, techniques and infrastructure to allow the deployment of privacy-aware IT systems, in which humans are integral part of the organizational processes and accountable

  4. Towards Data Protection Compliance

    NARCIS (Netherlands)

    Zannone, Nicola; Petkovic, M.; Etalle, Sandro

    2010-01-01

    Privacy and data protection are fundamental issues nowadays for every organization. This paper calls for the development of methods, techniques and infrastructure to allow the deployment of privacy-aware IT systems, in which humans are integral part of the organizational processes and accountable

  5. ISO radiation protection standards

    International Nuclear Information System (INIS)

    Becker, K.; West, N.

    1981-01-01

    After a brief description of the International Organization for Standardization (ISO) and its Technical Committee (TC) 85 ''Nuclear Energy'', the work of its Sub-Committee (SC) 2 ''Radiation Protection'' is described in some detail. Several international standards on subjects closely related to radiation protection have already been published, for example ISO-361 (Basic radiation protection symbol), ISO-1757 (Photographic dosimeters), ISO-1758 and 1759 (Direct and indirect-reading pocket exposure meters), ISO-2889 (Sampling of airborne radioactive materials), ISO-4037 (X and gamma reference radiations for calibration) and ISO-4071 (Testing of exposure meters and dosimeters). TC 85/SC 2 has currently eight active Working Groups (WG) dealing with 14 standards projects, mostly in advanced stages, in such fields as neutron and beta reference radiations, and X and gamma radiations of high and low dose-rates and high energies for calibration purposes, reference radiations for surface contamination apparatus, ejection systems for gamma radiography apparatus, industrial and laboratory irradiators, lead shielding units, protective clothing, thermoluminescence dosemeters, radioelement gauges, and surface contamination and decontamination. (author)

  6. Radiation protection in Qatar

    International Nuclear Information System (INIS)

    Al Maadheed, Khalid; Al Khatibeh, Ahmad

    2008-01-01

    Full text: The State of Qatar has become a member State of IAEA since 1974. Later the Department of Industrial Development (DID) beam the focal point and the competent authority regarding all aspects of the peaceful application of Nuclear Technology. In July, 2000 the Supreme Council was established and charged with all matters related to environmental protection. The Supreme Council joined the IAEA Projects on upgrading protection infrastructure in West Asia region. A preliminary research was initiated to discover where radiation sources are being used, and the legal framework, if any, to regulate their use. The research indicated that radiation sources were being used in the industrial practices (well logging, industrial radiography and nuclear gauges) and in medical practices (mainly diagnostic radiology). The research also indicated that there was virtually no legal framework to regulate them. In less than five years, the State of Qatar was able to issue the radiation protection law, three sets of regulations, namely: Radiation Protection Regulations, Radioactive Waste Management Regulations and the Safe Transport of Radioactive Materials Regulations. In addition, several specific regulation work, dose limits and radiation protection officers were issued. A radiation Protection Department, comprising three sections was established. We are providing individual exposure monitoring for most of the radiation workers in the public sector and some in the private sector. We have set up a proper licensing and inspections procedures, where our inspectors are enforcing the law. More recently, we established an early warning network for nuclear of radiological emergencies, consisting of 6 transplantable stations, five mobile stations and two navigating stations. This year, the network was augmented with five fixed station and an advanced early warning centre, which provides early warning via multiple means (MMS, Fax, E-mail and audio alarms). Last year we signed a nuclear

  7. Foundations for radiation protection

    International Nuclear Information System (INIS)

    2006-01-01

    Full text; In 1996, the IAEA published the latest edition of the International Basic Safety Standards for Protection Against Ionizing Radiation and for the Safety of Radiation Sources (Basic Safety Standards or BSS) comprising basic requirements to be filled in all activities involving radiation exposure. The standards define internationally harmonized requirements and provide practical guidance for public authorities and services, employers and workers, specialized radiation protection bodies, enterprises and health and safety communities. In the same year, the IAEA, through the technical cooperation programme, launched the Model Project on Upgrading Radiation Protection Infrastructure, a global initiative designed to help Member States establish the infrastructure needed to adhere to the BSS. To address the complexity of this task, the radiation protection team identified key elements, known as Thematic Safety Areas. These are: 1. Legislative Framework and Regulatory Infrastructure, Draft and put into effect radiation protection laws and regulations and establish and empower a national regulatory authority. 2. Occupational Exposure Control Protect the health and safety of each individual who faces the risk of radiation exposure in the workplace through individual and workplace monitoring programmes, including dose assessment, record keeping of doses and quality management. 3. Medical Exposure Control: Develop procedures and activities to control the exposure of patients undergoing diagnosis and/or treatment via diagnostic and interventional radiology, nuclear medicine or radiotherapy through staff training, provision of basic quality control equipment, and the establishment of quality assurance programmes. 4. Public and Environmental Exposure Control: Develop means to protect both the public and the environment including: a) programmes to register, inventory and provide safe storage of unused radioactive sources and material; b) procedures to control and safely

  8. Principles of Radiation Protection Concepts

    International Nuclear Information System (INIS)

    Abd Aziz Mhd Ramli

    2004-01-01

    The contents of this chapter are follows - Radiation Protection Concepts: justification, dose limitation, optimisation, potential exposures, situation requiring intervention; Special Considerations. Protection from Radiation Hazards, Remove the Hazard, Prevent the Hazard, Guard the Worker, Implementation of Radiation Protection and Safety Measures, Distance, Shielding, Time, Monitoring Programme, Safety System. Radiation Protection in Radiological Service: Specific Requirement in Diagnostic Radiological Service

  9. BGS Radon Protective Measures GIS

    International Nuclear Information System (INIS)

    Appleton, D.; Adlam, K.

    2000-01-01

    The British Geological Survey Radon Protective Measures Geographical Information System is described. The following issues are highlighted: Identification of development sites where radon protection is required in new dwellings; Mapping radon potential on the basis of house radon and geology; Radon Protective Measures GIS; Radon site reports; and Follow-up radon protective measures sire reports

  10. Protection of HTS magnets

    International Nuclear Information System (INIS)

    Iwasa, Yukikazu

    2005-01-01

    The paper discusses protection issues for HTS magnet. For protection, the HTS magnet must rely on an active technique. Closed-form expressions of the matrix metal operating current density based on overheating and internal voltage criteria for protection, under very simplifying assumptions, are presented. Perhaps the most important conclusions of these criteria are that: (1) HTS (and LTS) magnets must be wound with composite conductor having a significant portion of its overall cross section occupied by normal metal generally of high electrical conductivity and (2) HTS windings must possess 'high' NZP velocities to make the resistive zone occupy as large a fraction of the winding volume as possible. The paper also derives an analytical expression, under another set of simplifying assumptions, to determine the minimum resistive voltage level, dictated by the maximum hot-spot temperature set at 150 K, required to initiate an active protection process. Remarkably, this minimum detection voltage is nearly independent of the matrix metal current density, I op /A m . For a set of operating parameters used in a numerical example, a computed minimum detection voltage, at I op /A m = 5 x 10 4 A/cm 2 , is ∼30 mV, which, considering it must be discerned in the presence of extraneous voltage signals likely to be present in real world operating conditions, would be non-trivial. To satisfy the overheating criterion at a level of I op /A m , which keeps the winding overall current density 'viable' and at the same time to raise the minimum detection resistive voltage, the winding volume occupied by the resistive state must be expanded. The paper concludes with discussion of challenging new areas of research for protection of HTS magnets

  11. Enhancing radiation protection

    International Nuclear Information System (INIS)

    2006-01-01

    When a new radiotherapy center in Gezira, Sudan, delivers its first therapeutic dose to a cancer patient, two things happen: A young man begins to regain his health and looks forward to being better able to support his family and contribute to his community; and a developing nation realizes an important step toward deriving the social and economic benefits of nuclear science. The strategic application of nuclear technology in particular fields- human health, industry, food and agriculture, energy, water resources and environmental protection - has enormous potential to help shape the future of developing countries. But past radiological incidents, several of which involved high levels of exposure or death (Bolivia, Brazil, Cost Rica, Georgia, Ghana, Morocco, Panama and Thailand), underscore the inherent and very serious risks. For this reason, the IAEA's Departments of Technical Cooperation and Nuclear Safety and Security partner closely, particularly in the area of radiation protection. They strive to consider every minute detail in the equation that brings together radiation sources, modern technologies, people and the environment. Launched in 1996, the Model Project on Upgrading Radiation Protection Infrastructure (the Model Project) aimed to help Member States: achieve capacities that underpin the safe and secure application of nuclear technologies; establish a legislative framework and regulatory infrastructure; develop exposure control mechanisms to protect workers, medical patients, the public and the environment; and achieve preparedness and planned response to radiological emergencies. In fact, the hospital scenario above typically marks several years of intense collaboration amongst scientists, legislators, regulators, politicians and administrators from both Member States and the IAEA, orchestrated and aided by regional managers and technical experts from the IAEA. As radiation protection team members can attest, every application of nuclear technology

  12. Focus radiation protection; Schwerpunkt Strahlenschutz

    Energy Technology Data Exchange (ETDEWEB)

    Ebermann, Lutz (comp.)

    2016-07-01

    The publication of the Bundesamt fuer Strahlenschutz on radiation protection covers the following issues: (i) exposure from natural sources: health hazard due to radon, radiation protection in residential homes, radon in Germany, natural raw materials in industrial processes; (ii) clearance of radioactive wastes: clearance in the frame of nuclear power plant dismantling, the situation in Germany and Europe; (iii) emergency management: principles of radiation protection, fictive sequence of accident events; (iiii) other actual radiation protection topics: more limits - more protection? radiation protection in medicine, occupational radiation protection.

  13. Digital protection in power plants. Electrical unit and line protection. Digital protection systems for NPP

    International Nuclear Information System (INIS)

    Kaczmarek, A.

    2000-01-01

    In this presentation author deals with the digital protection systems for nuclear power plants. The evolution of protection devices, protection concept for power plants, concept of functional redundancy, references for digital protection, benefits for the customer well as concept fault recorder are presented. (author)

  14. Temporary physical protection systems

    International Nuclear Information System (INIS)

    Williams, J.D.; Gangel, D.J.; Madsen, R.W.

    1991-01-01

    Terrorism and other aspects of world political instability have created a high demand for temporary physical protection systems within the nuclear materials management community. They can be used when vehicles carrying important assets are away from their permanent fixed site location, around areas where experiments are being temporarily conducted, around construction areas and one portions of a fixed site physical security system which is temporarily inoperable. Physical security systems can be grouped into four categories: tactical, portable, semi-permanent, and fixed. The resources and experience gained at Sandia National Laboratories in over forty years of developing and implementing security systems for protecting nuclear weapons and fixed nuclear facilities is now being applied to temporary physical security systems. This paper emphasizes temporary physical security systems and their component parts that are presently available and identify additional system-subsystem objectives, requirements, and concepts

  15. Radiation protection manual

    International Nuclear Information System (INIS)

    Spang, A.

    1983-01-01

    According to the Radiation Protection Ordinance, radiation protection experts directing or supervising the handling of radioactive materials must have expert knowledge. The concept of expert knowledge has been clearly defined by the Fachverband e.V. in a catalogue of instruction goals. The manual follows the principles of this catalogue; it presents the expert knowledge required in a total of 15 subject groups. There is an index which helps the reader to find his specific subject group and the knowledge required of him in this subject group. However, the manual gives only an outline of the subject matter in many instances and should therefore not be regarded as a textbook in the proper sense. (orig./HP) [de

  16. Radiation Protection. Chapter 24

    Energy Technology Data Exchange (ETDEWEB)

    Sutton, D. [Ninewells Hospital, Dundee (United Kingdom); Collins, L. T. [Westmead Hospital, Sydney (Australia); Le Heron, J. [International Atomic Energy Agency, Vienna (Austria)

    2014-09-15

    Chapter 21, in describing basic radiation biology and radiation effects, demonstrates the need to have a system of radiation protection that allows the many beneficial uses of radiation to be realized while ensuring detrimental radiation effects are either prevented or minimized. This can be achieved with the twin objectives of preventing the occurrence of deterministic effects and of limiting the probability of stochastic effects to a level that is considered acceptable. In a radiology facility, consideration needs to be given to the patient, the staff involved in performing the radiological procedures, members of the public and other staff that may be in the radiology facility, carers and comforters of patients undergoing procedures, and persons who may be undergoing a radiological procedure as part of a biomedical research project. This chapter discusses how the objectives given above are fulfilled through a system of radiation protection and how such a system should be applied practically in a radiology facility.

  17. EMP protection technology interchange

    International Nuclear Information System (INIS)

    Martin, L.C.

    1977-01-01

    Investigation and assessment of the potential degrading and destructive effects associated with the nuclear electromagnetic (EMP) phenomena has created a large technological base. This base is indeed quite broad owing to the unique and system-pervasive character of the EMP. One of the aims of an emerging discipline, such as that associated with EMP, is to provide for adequate transfer and integration of the developing technology into the overall engineering community. The needs of the community and how EMP hardening and protection information can be transferred or exchanged are reviewed. Many of the current and generally available sources of information are listed, examples of interchange methods are provided, and problems of developing specifications, standards, and suitable guidelines for design and incorporation of protection are also discussed

  18. Lectures on radiation protection

    International Nuclear Information System (INIS)

    Wachsmann, F.; Consentius, K.

    1981-01-01

    All important subjects of radiation protection are presented in concise form; the explanations may serve as lecture manuscripts. The lectures are divided into 16 to 19 teaching units. Each teaching unit is supplemented by a slide to be projected on a screen while the text is read. This method of visual teaching has already been tried with good results in medicine and medical engineering. Pictures of the slides are given in the text so that the book may also be used for self-studies. The main facts are summarized at the end of each lesson. The finished book will consist of 8 lessons; the first three of these discuss 1. Radiation effects and hazards 2. Dose definitions and units and their role in radiology and radiation protection 3. Dose limits and legal specifications. (orig.) [de

  19. Radiation protection zoning

    International Nuclear Information System (INIS)

    2015-01-01

    Radiation being not visible, the zoning of an area containing radioactive sources is important in terms of safety. Concerning radiation protection, 2 work zones are defined by regulations: the monitored zone and the controlled zone. The ministerial order of 15 may 2006 settles the frontier between the 2 zones in terms of radiation dose rates, the rules for access and the safety standards in both zones. Radioprotection rules and the name of the person responsible for radiation protection must be displayed. The frontier between the 2 zones must be materialized and marked with adequate equipment (specific danger signs and tapes). Both zones are submitted to selective entrance, the access for the controlled zone is limited because of the radiation risk and of the necessity of confining radioactive contamination while the limitation of the access to the monitored zone is due to radiation risk only. (A.C.)

  20. Superconducting coil protection

    International Nuclear Information System (INIS)

    Woods, E.L.

    1975-01-01

    The protection system is based on a two-phase construction program. Phase I is the development of a reliable hardwired relay control system with a digital loop utilizing firmware and a microprocessor controller. Phase II is an expansion of the digital loop to include many heretofore unmonitored coil variables. These new monitored variables will be utilized to establish early quench detection and to formulate confirmation techniques of the quench detection mechanism. Established quench detection methods are discussed and a new approach to quench detection is presented. The new circuit is insensitive to external pulsed magnetic fields and the associated induced voltages. Reliability aspects of the coil protection system are discussed with respect to shutdowns of superconducting coil systems. Redundance and digital system methods are presented as related topics