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Sample records for rhodamines

  1. Studying reactions of interaction of tungsten (6) with rhodamine B and rhodamine 6 Zh

    International Nuclear Information System (INIS)

    Andreeva, I.Yu.; Lebedeva, L.I.; Burmistrova, N.M.

    1978-01-01

    The reaction of the interaction between tungsten (6) and rhodamine B and rhodamine 6 Zh has been investigated. The formation of two compounds in the tungsten-rhodamine system is shown. The composition of these compounds has been determined, and their stability has been estimated. The composition of compounds in the solid phase has also been determined. This reaction is used for the determination of tungsten in stells

  2. Superradiant laser emission from rhodamine 6G and rhodamine B using a coaxial flashpump.

    Science.gov (United States)

    Mumola, P. B.

    1972-01-01

    Superradiant laser emission has been observed from ethanol solutions of rhodamine 6G and rhodamine B using a coaxial flashlamp pump. The dependence of input threshold energy on dye concentration is examined. The possibility of exciting other dyes to superradiance is discussed.

  3. Quirks of dye nomenclature. 5. Rhodamines.

    Science.gov (United States)

    Cooksey, C J

    2016-01-01

    Rhodamines were first produced in the late 19(th) century, when they constituted a new class of synthetic dyes. These compounds since have been used to color many things including cosmetics, inks, textiles, and in some countries, food products. Certain rhodamine dyes also have been used to stain biological specimens and currently are widely used as fluorescent probes for mitochondria in living cells. The early history and current biological applications are sketched briefly and an account of the ambiguities, complications and confusions concerning dye identification and nomenclature are discussed.

  4. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats.

    Science.gov (United States)

    Jiang, Shiau-Han; Cheng, Yung-Yi; Huo, Teh-Ia; Tsai, Tung-Hu

    2017-09-06

    Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, and sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats. Rhodamine 110 exhibited linear pharmacokinetics and slow elimination after oral administration. Furthermore, its oral bioavailability was approximately 34-35%. The distribution in the liver and kidney suggests that these organs are primarily responsible for rhodamine 110 metabolism and elimination. Our investigation describes the pharmacokinetics and a quantification method for rhodamine 110, improving our understanding of the food safety of rhodamine dyes.

  5. (18)F-labeled rhodamines as potential myocardial perfusion agents: comparison of pharmacokinetic properties of several rhodamines.

    Science.gov (United States)

    Bartholomä, Mark D; Zhang, Shaohui; Akurathi, Vamsidhar; Pacak, Christina A; Dunning, Patricia; Fahey, Frederic H; Cowan, Douglas B; Treves, S Ted; Packard, Alan B

    2015-10-01

    We recently reported the development of the [(18)F]fluorodiethylene glycol ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging (MPI). This compound was developed by optimizing the ester moiety on the rhodamine B core, and its pharmacokinetic properties were found to be superior to those of the prototype ethyl ester. The goal of the present study was to optimize the rhodamine core while retaining the fluorodiethyleneglycol ester prosthetic group. A series of different rhodamine cores (rhodamine 6G, rhodamine 101, and tetramethylrhodamine) were labeled with (18)F using the corresponding rhodamine lactones as the precursors and [(18)F]fluorodiethylene glycol ester as the prosthetic group. The compounds were purified by semipreparative HPLC, and their biodistribution was measured in rats. Additionally, the uptake of the compounds was evaluated in isolated rat cardiomyocytes. As was the case with the different prosthetic groups, we found that the rhodamine core has a significant effect on the in vitro and in vivo properties of this series of compounds. Of the rhodamines evaluated to date, the pharmacologic properties of the (18)F-labeled diethylene glycol ester of rhodamine 6G are superior to those of the (18)F-labeled diethylene glycol esters of rhodamine B, rhodamine 101, and tetramethylrhodamine. As with (18)F-labeled rhodamine B, [(18)F]rhodamine 6G was observed to localize in the mitochondria of isolated rat cardiomyocytes. Based on these results, the (18)F-labeled diethylene glycol ester of rhodamine 6G is the most promising potential PET MPI radiopharmaceutical of those that have evaluated to date, and we are now preparing to carry out first-in-human clinical studies with this compound. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. 18F-labeled rhodamines as potential myocardial perfusion agents: comparison of pharmacokinetic properties of several rhodamines

    International Nuclear Information System (INIS)

    Bartholomä, Mark D.; Zhang, Shaohui; Akurathi, Vamsidhar; Pacak, Christina A.; Dunning, Patricia; Fahey, Frederic H.; Cowan, Douglas B.; Ted Treves, S.; Packard, Alan B.

    2015-01-01

    Introduction: We recently reported the development of the [ 18 F]fluorodiethylene glycol ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging (MPI). This compound was developed by optimizing the ester moiety on the rhodamine B core, and its pharmacokinetic properties were found to be superior to those of the prototype ethyl ester. The goal of the present study was to optimize the rhodamine core while retaining the fluorodiethyleneglycol ester prosthetic group. Methods: A series of different rhodamine cores (rhodamine 6G, rhodamine 101, and tetramethylrhodamine) were labeled with 18 F using the corresponding rhodamine lactones as the precursors and [ 18 F]fluorodiethylene glycol ester as the prosthetic group. The compounds were purified by semipreparative HPLC, and their biodistribution was measured in rats. Additionally, the uptake of the compounds was evaluated in isolated rat cardiomyocytes. Results: As was the case with the different prosthetic groups, we found that the rhodamine core has a significant effect on the in vitro and in vivo properties of this series of compounds. Of the rhodamines evaluated to date, the pharmacologic properties of the 18 F-labeled diethylene glycol ester of rhodamine 6G are superior to those of the 18 F-labeled diethylene glycol esters of rhodamine B, rhodamine 101, and tetramethylrhodamine. As with 18 F-labeled rhodamine B, [ 18 F]rhodamine 6G was observed to localize in the mitochondria of isolated rat cardiomyocytes. Conclusions: Based on these results, the 18 F-labeled diethylene glycol ester of rhodamine 6G is the most promising potential PET MPI radiopharmaceutical of those that have evaluated to date, and we are now preparing to carry out first-in-human clinical studies with this compound

  7. 18F-labeled Rhodamines as Potential Myocardial Perfusion Agents: Comparison of Pharmacokinetic Properties of Several Rhodamines

    Science.gov (United States)

    Bartholoma, Mark D.; Zhang, Shaohui; Akurathi, Vamsidhar; Pacak, Christina A.; Dunning, Patricia; Fahey, Frederic H.; Cowan, Douglas B.; Treves, S. Ted; Packard, Alan B.

    2015-01-01

    Introduction We recently reported the development of the [18F]fluorodiethylene glycol ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging (MPI). This compound was developed by optimizing the ester moiety on the rhodamine B core, and its pharmacokinetic properties were found to be superior to those of the prototype ethyl ester. The goal of the present study was to optimize the rhodamine core while retaining the fluorodiethyleneglycol ester prosthetic group. Methods A series of different rhodamine cores (rhodamine 6G, rhodamine 101, and tetramethylrhodamine) were labeled with 18F using the corresponding rhodamine lactones as the precursors and [18F]fluorodiethylene glycol ester as the prosthetic group. The compounds were purified by semipreparative HPLC, and their biodistribution was measured in rats. Additionally, the uptake of the compounds was evaluated in isolated rat cardiomyocytes. Results As was the case with the different prosthetic groups, we found that the rhodamine core has a significant effect on the in vitro and in vivo properties of this series of compounds. Of the rhodamines evaluated to date, the pharmacologic properties of the 18F-labeled diethylene glycol ester of rhodamine 6G are superior to those of the 18F-labeled diethylene glycol esters of rhodamine B, rhodamine 101, and tetramethylrhodamine. As with 18F-labeled rhodamine B, [18F]rhodamine 6G was observed to localize in the mitochondria of isolated rat cardiomyocytes. Conclusions Based on these results, the 18F-labeled diethylene glycol ester of rhodamine 6G is the most promising potential PET MPI radiopharmaceutical of those that have been evaluated to date, and we are now preparing to carry out first-in-human clinical studies with this compound. PMID:26205075

  8. Pulse radiolysis of rhodamine dye solutions

    International Nuclear Information System (INIS)

    Kucherenko, E.A.; Kartasheva, L.I.; Pikaev, A.K.

    1982-01-01

    Applying the method of pulse radiolysis (5 MeV electrons) a study was made on intermediate products of rhodamine B radiolytic transformations in neutral aqueous and ethanol solutions. Rate constants of reactions of esub(aq) and OH with the dye (they are equal to (2.2+-0.3)x10 10 and (2.1+-0.3)x10 10 e/molxs, accordingly) as well as optical and kinetic characteristics of esub(aq), OH and H interaction products were measured. The nature of these products is concluded. It was found that in ethanol solutions the semirecovered form - electroneutral radical of rhodamine B - was the only intermediate product. It arises during the interaction of the dye with esub(s) (k=(9.2+-1.2)x10 9 e/molxs) and α-et hananol radical (k=(1.1+-0.1)x10 8 l/molxs). Properties of this product were investigated

  9. Photolysis of rhodamine-WT dye

    Science.gov (United States)

    Tai, D.Y.; Rathbun, R.E.

    1988-01-01

    Photolysis of rhodamine-WT dye under natural sunlight conditions was determined by measuring the loss of fluorescence as a function of time. Rate coefficients at 30?? north latitude ranged from 4.77 x 10-2 day-1 for summer to 3.16 x 10-2 day-1 for winter. Experimental coefficients were in good agreement with values calculated using a laboratory-determined value of the quantum yield.

  10. Radioiodinated Rhodamine-123: a potential cationic hepatobiliary imaging agent

    International Nuclear Information System (INIS)

    Moonen, P.; Gorree, G.C.M.; Hoekstra, A.

    1987-01-01

    The labelling of the cationic dye Rhodamine-123 with 125 I is described. The biodistribution of the iodinated Rhodamine-123 has been determined at different time intervals after intravenous injection into fasted rats. It turned out that the dye is predominantly cleared by the liver and discharged into the bile. The bile acid taurocholate did not enhance the rate of excretion of 125 I-Rhodamine-123. (author)

  11. Blocking of Single α-Hemolysin Pore by Rhodamine Derivatives.

    Science.gov (United States)

    Rokitskaya, Tatyana I; Nazarov, Pavel A; Golovin, Andrey V; Antonenko, Yuri N

    2017-06-06

    Measurements of ion conductance through α-hemolysin pore in a bilayer lipid membrane revealed blocking of the ion channel by a series of rhodamine 19 and rhodamine B esters. The longest dwell closed time of the blocking was observed with rhodamine 19 butyl ester (C4R1), whereas the octyl ester (C8R1) was of poor effect. Voltage asymmetry in the binding kinetics indicated that rhodamine derivatives bound to the stem part of the aqueous pore lumen. The binding frequency was proportional to a quadratic function of rhodamine concentrations, thereby showing that the dominant binding species were rhodamine dimers. Two levels of the pore conductance and two dwell closed times of the pore were found. The dwell closed times lengthened as the voltage increased, suggesting impermeability of the channel for the ligands. Molecular docking analysis revealed two distinct binding sites within the lumen of the stem of the α-hemolysin pore for the C4R1 dimer, but only one binding site for the C8R1 dimer. The blocking of the α-hemolysin nanopore by rhodamines could be utilized in DNA sequencing as additional optical sensing owing to bright fluorescence of rhodamines if used for DNA labeling. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  12. Rhodamine-WT dye losses in a mountain stream environment

    Science.gov (United States)

    Bencala, Kenneth E.; Rathburn, Ronald E.; Jackman, Alan P.; Kennedy, Vance C.; Zellweger, Gary W.; Avanzino, Ronald J.

    1983-01-01

    A significant fraction of rhodamine WT dye was lost during a short term multitracer injection experiment in a mountain stream environment. The conservative anion chloride and the sorbing cation lithium were concurrently injected. In-stream rhodamine WT concentrations were as low as 45 percent of that expected, based on chloride data. Concentration data were available from shallow‘wells’dug near the stream course and from a seep of suspected return flow. Both rhodamine WT dye and lithium were nonconservative with respect to the conservative chloride, with rhodamine WT dye closely following the behavior of the sorbing lithium.Nonsorption and sorption mechanisms for rhodamine WT loss in a mountain stream were evaluated in laboratory experiments. Experiments evaluating nonsorption losses indicated minimal losses by such mechanisms. Laboratory experiments using sand and gravel size streambed sediments show an appreciable capacity for rhodamine WT sorption.The detection of tracers in the shallow wells and seep indicates interaction between the stream and the flow in the surrounding subsurface, intergravel water, system. The injected tracers had ample opportunity for intimate contact with materials shown in the laboratory experiments to be potentially sorptive. It is suggested that in the study stream system, interaction with streambed gravel was a significant mechanism for the attenuation of rhodamine WT dye (relative to chloride).

  13. [11 C]Rhodamine-123: Synthesis and biodistribution in rodents

    International Nuclear Information System (INIS)

    Bao Xiaofeng; Lu Shuiyu; Liow, Jeih-San; Morse, Cheryl L.; Anderson, Kacey B.; Zoghbi, Sami S.; Innis, Robert B.; Pike, Victor W.

    2012-01-01

    Introduction: Rhodamine-123 is a known substrate for the efflux transporter, P-glycoprotein (P-gp). We wished to assess whether rhodamine-123 might serve as a useful substrate for developing probes for imaging efflux transporters in vivo with positron emission tomography (PET). For this purpose, we aimed to label rhodamine-123 with carbon-11 (t 1/2 = 20.4 min) and to study its biodistribution in rodents. Methods: [ 11 C]Rhodamine-123 was prepared by treating rhodamine-110 (desmethyl-rhodamine-123) with [ 11 C]methyl iodide. The biodistribution of this radiotracer was studied with PET in wild-type mice and rats, in efflux transporter knockout mice, in wild-type rats pretreated with DCPQ (an inhibitor of P-gp) or with cimetidine (an inhibitor of organic cation transporters; OCT), and in P-gp knockout mice pretreated with cimetidine. Unchanged radiotracer in forebrain, plasma and peripheral tissues was also measured ex vivo at 30 min after radiotracer administration to wild-type and efflux transporter knockout rodents. Results: [ 11 C]Rhodamine-123 was obtained in 4.4% decay-corrected radiochemical yield from cyclotron-produced [ 11 C]carbon dioxide. After intravenous administration of [ 11 C]rhodamine-123 to wild-type rodents, PET and ex vivo measurements showed radioactivity uptake was very low in brain, but relatively high in some other organs such as heart, and especially liver and kidney. Inhibition of P-gp increased uptake in brain, heart, kidney and liver, but only by up to twofold. Secretion of radioactivity from kidney was markedly reduced by OCT knockout or pretreatment with cimetidine. Conclusions: [ 11 C]Rhodamine-123 was unpromising as a PET probe for P-gp function and appears to be a strong substrate of OCT in kidney. Cimetidine appears effective for blocking OCT in kidney in vivo.

  14. Localization of mitochondria in living cells with rhodamine 123.

    Science.gov (United States)

    Johnson, L V; Walsh, M L; Chen, L B

    1980-01-01

    The laser dye rhodamine 123 is shown to be a specific probe for the localization of mitochondria in living cells. By virtue of its selectivity for mitochondria and its fluorescent properties, the detectability of mitochondria stained with rhodamine 123 is significantly improved over that provided by conventional light microscopic techniques. With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment. Images PMID:6965798

  15. Pulse radiolysis of ethanolic solutions of rhodamine dyes

    International Nuclear Information System (INIS)

    Kartasheva, L.I.; Kucherenko, E.A.; Kozlov, A.S.; Pikaev, A.K.

    1983-01-01

    The primary products of radiolytical transformations of rhodamine 6G, rhodamine B, rhodamine 3B and rhodamine 110 in ethanolic solutions were studied by pulse radiolysis method under various conditions. It was found that the semireduced form of a dye was the only intermediate product of such transformations in ethanolic solutions of all dyes. It was shown that this species was formed by interaction of the dye with esub(s) - and CH 3 CHOH. The properties of this species were investigated and the rate constants of respective reactions for each dye were determined. It was found that nature and position of a substituent in the molecule of the dye have an effect on the rate of formation of the semi-reduced form. (author)

  16. Identifikasi Zat Warna Rhodamine B Pada Lipstik Berwarna Merah

    OpenAIRE

    A.H., Mukaromah; E.T., Maharani

    2008-01-01

    Telah dilakukan penelitian tentang identifikasi zat warna Rhodamine B pada lipsttk. Populasi Penelitian adalah sediaan lipstik yang berwarna merah dengan harga kurang atau sama dengan Rp 5000,00 (12 merk) dan sediaan lipstik yang berwarna merah dengan harga lebih Rp 5000,00 (13 merk) yang dijual di Pasar Johar Semarang. Masing-masing sediaan lipstik diambil 40 % dari total populasi secara acak. Metode yang digunakan untuk mangidentifikasi zat warna Rhodamine B adalah metode kromatograf...

  17. Noninvasive control of rhodamine-loaded capsules distribution in vivo

    Science.gov (United States)

    Stelmashchuk, O.; Tarakanchikova, Y.; Seryogina, E.; Piavchenko, G.; Zherebtsov, E.; Dunaev, A.; Popov, A.; Meglinski, I.

    2018-04-01

    Using fluorescence spectroscopy system with fibre-optical probe, we investigated the dynamics of propagation and circulation in the microcirculatory system of experimental nanocapsules fluorescent-labelled (rhodamine TRITC) nanocapsules. The studies were carried out in clinically healthy Wistar rats. The model animals were divided into control group and group received injections of the nanocapsules. The fluorescent measurements conducted transcutaneously on the thigh surface. The administration of the preparation with the rhodamine concentration of 5 mg/kg of animal weight resulted in twofold increase of fluorescence intensity by reference to the baseline level. As a result of the study, it was concluded that fluorescence spectroscopy can be used for transdermal measurements of the rhodamine-loaded capsules in vivo.

  18. Hydrophobic analogues of rhodamine B and rhodamine 101: potent fluorescent probes of mitochondria in living C. elegans

    Directory of Open Access Journals (Sweden)

    Laurie F. Mottram

    2012-12-01

    Full Text Available Mitochondria undergo dynamic fusion and fission events that affect the structure and function of these critical energy-producing cellular organelles. Defects in these dynamic processes have been implicated in a wide range of human diseases including ischemia, neurodegeneration, metabolic disease, and cancer. To provide new tools for imaging of mitochondria in vivo, we synthesized novel hydrophobic analogues of the red fluorescent dyes rhodamine B and rhodamine 101 that replace the carboxylate with a methyl group. Compared to the parent compounds, methyl analogues termed HRB and HR101 exhibit slightly red-shifted absorbance and emission spectra (5–9 nm, modest reductions in molar extinction coefficent and quantum yield, and enhanced partitioning into octanol compared with aqueous buffer of 10-fold or more. Comparison of living C. elegans (nematode roundworm animals treated with the classic fluorescent mitochondrial stains rhodamine 123, rhodamine 6G, and rhodamine B, as well as the structurally related fluorophores rhodamine 101, and basic violet 11, revealed that HRB and HR101 are the most potent mitochondrial probes, enabling imaging of mitochondrial motility, fusion, and fission in the germline and other tissues by confocal laser scanning microscopy after treatment for 2 h at concentrations as low as 100 picomolar. Because transgenes are poorly expressed in the germline of these animals, these small molecules represent superior tools for labeling dynamic mitochondria in this tissue compared with the expression of mitochondria-targeted fluorescent proteins. The high bioavailabilty of these novel fluorescent probes may facilitate the identification of agents and factors that affect diverse aspects of mitochondrial biology in vivo.

  19. Synthesis of Rhodamines from Fluoresceins Using Pd-Catalyzed C–N Cross-Coupling

    Science.gov (United States)

    2011-01-01

    A unified, convenient, and efficient strategy for the preparation of rhodamines and N,N′-diacylated rhodamines has been developed. Fluorescein ditriflates were found to undergo palladium-catalyzed C–N cross-coupling with amines, amides, carbamates, and other nitrogen nucleophiles to provide direct access to known and novel rhodamine derivatives, including fluorescent dyes, quenchers, and latent fluorophores. PMID:22091952

  20. Photostabilization of Rhodamine 6G in acetone by β-carotene and t-butyl hydroxyanisole

    OpenAIRE

    Aoki, Keisuke; Matsuda, Masahiro; Yamamoto, Daisuke; Abe, Yasuhiro; Akutsu, Shinya; Fujita, Koichi; Doi, Kazutaka; Kobayashi, Masami

    2011-01-01

    The photostability of Rhodamine 6G is of crucial importance for the organic light-emitting display of mobilephones. Photobleaching of Rhodamine 6G in acetone was not protected by β-carotene, but was efficientlyphotostabilized by t-butyl hydroxyanisole. The results indicate that photobleaching of Rhodamine 6G inacetone was not caused by singlet oxygen but some radical species.

  1. Synthesis and characterization of macromolecular rhodamine tethers and their interactions with P-glycoprotein.

    Science.gov (United States)

    Crawford, Lindsey; Putnam, David

    2014-08-20

    Rhodamine dyes are well-known P-glycoprotein (P-gp) substrates that have played an important role in the detection of inhibitors and other substrates of P-gp, as well as in the understanding of P-gp function. Macromolecular conjugates of rhodamines could prove useful as tethers for further probing of P-gp structure and function. Two macromolecular derivatives of rhodamine, methoxypolyethylene glycol-rhodamine6G and methoxypolyethylene glycol-rhodamine123, were synthesized through the 2'-position of rhodamine6G and rhodamine123, thoroughly characterized, and then evaluated by inhibition with verapamil for their ability to interact with P-gp and to act as efflux substrates. To put the results into context, the P-gp interactions of the new conjugates were compared to the commercially available methoxypolyethylene glycol-rhodamineB. FACS analysis confirmed that macromolecular tethers of rhodamine6G, rhodamine123, and rhodamineB were accumulated in P-gp expressing cells 5.2 ± 0.3%, 26.2 ± 4%, and 64.2 ± 6%, respectively, compared to a sensitive cell line that does not overexpress P-gp. Along with confocal imaging, the efflux analysis confirmed that the macromolecular rhodamine tethers remain P-gp substrates. These results open potential avenues for new ways to probe the function of P-gp both in vitro and in vivo.

  2. Spiroguanidine rhodamines as fluorogenic probes for lysophosphatidic acid

    Science.gov (United States)

    Wang, Lei; Sibrian-Vazquez, Martha; Escobedo, Jorge O.; Wang, Jialu; Moore, Richard G.

    2015-01-01

    Direct determination of total lysophosphatidic acid (LPA) was accomplished using newly developed spiroguanidines derived from rhodamine B as universal fluorogenic probes. Optimum conditions for the quantitative analysis of total LPA were investigated. The linear range for the determination of total LPA is up to 5 μM with a limit of detection of 0.512 μM. PMID:25516957

  3. Pharmacokinetics and Biodistribution of the Illegal Food Colorant Rhodamine B in Rats.

    Science.gov (United States)

    Cheng, Yung-Yi; Tsai, Tung-Hu

    2017-02-08

    The International Agency for Research on Cancer (IARC) demonstrated rhodamine B as a potential carcinogen in 1978. Nevertheless, rhodamine B has been illegally used as a colorant in food in many countries. Few pharmacokinetic and toxicological investigations have been performed since the first pharmacokinetic study on rhodamine B in 1961. The aims of this study were to develop a simple and sensitive high-performance liquid chromatography method with fluorescence detection for the quantitative detection of rhodamine B in the plasma and organs of rats and to estimate its pharmacokinetics and biodistribution. The results demonstrated that the oral bioavailabilities of rhodamine B were 28.3 and 9.8% for the low-dose and high-dose exposures, respectively. Furthermore, rhodamine B was highly accumulated in the liver and, to a lesser extent, the kidney, but was undetectable in the brain. These results provide useful information for improving the pharmacokinetics and biodistribution of rhodamine B, supporting additional food safety evaluations.

  4. New fluorinated rhodamines for optical microscopy and nanoscopy.

    Science.gov (United States)

    Mitronova, Gyuzel Yu; Belov, Vladimir N; Bossi, Mariano L; Wurm, Christian A; Meyer, Lars; Medda, Rebecca; Moneron, Gael; Bretschneider, Stefan; Eggeling, Christian; Jakobs, Stefan; Hell, Stefan W

    2010-04-19

    New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to undergo sulfonation of the xanthene fragment at the positions 4' and 5'. Two fluorine atoms were introduced into the positions 2' and 7' of the 3',6'-diaminoxanthene fragment in compounds 1 a-d, 1 i-l and 1 m-r. The new rhodamine dyes may be excited with λ=488 or 514 nm light; most of them emit light at λ=512-554 nm (compounds 1 q and 1r at λ=576 and 589 nm in methanol, respectively) and have high fluorescence quantum yields in solution (up to 98 %), relatively long excited-state lifetimes (>3 ns) and are resistant against photobleaching, especially at high laser intensities, as is usually applied in confocal microscopy. Sulfonation of the xanthene fragment with 30 % SO3 in H2SO4 is compatible with the secondary amide bond (rhodamine-CON(Me)CH2CH2COOH) formed with MeNHCH2CH2COOCH3 to providing the sterically unhindered carboxylic group required for further (bio)conjugation reactions. After creating the amino reactive sites, the modified derivatives may be used as fluorescent markers and labels for (bio)molecules in optical microscopy and nanoscopy with very-high light intensities. Further, the new rhodamine dyes are able to pass the plasma membrane of living cells, introducing them as potential labels for recent live-cell-tag approaches. We exemplify the excellent performance of the fluorinated rhodamines in optical microscopy by fluorescence correlation spectroscopy (FCS) and stimulated emission depletion (STED) nanoscopy experiments. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Energy transfer in porous anodic alumina/rhodamine 110 nanocomposites

    Energy Technology Data Exchange (ETDEWEB)

    Elhouichet, H., E-mail: habib.elhouichet@fst.rnu.tn [Laboratoire de Physico-Chimie des Materiaux Mineraux et leurs Applications, Centre National de Recherches en Sciences des Materiaux, B.P. 95, Hammam-Lif 2050 (Tunisia); Departement de Physique, Faculte des Sciences de Tunis, University of Tunis Elmanar 2092 Tunis (Tunisia); Harima, N.; Koyama, H. [Hyogo University of Teacher Education, Kato, Hyogo 673-1494 (Japan); Gaponenko, N.V. [Belarusian State University of Informatics and Radioelectronics, P. Browki St. 6, 220013 Minsk (Belarus)

    2012-09-15

    We have used porous anodic alumina (PAA) films as templates for embedding rhodamine 110 (Rh110) molecules and examined their photoluminescence (PL) properties in detail. The analysis of the polarization memory (PM) of PL strongly suggests that there is a significant energy transfer from PAA to Rh110 molecules. The effect of annealing the PAA layer on the PL properties of the nanocomposite has been studied. The results show that the energy transfer becomes more efficient in annealed PAA. - Highlights: Black-Right-Pointing-Pointer Porous anodic alumina-rhodamine 110 nanocomposites are elaborated. Black-Right-Pointing-Pointer Efficient energy transfer from the host to Rh110 molecules is evidenced from measurements of photoluminescence and degree of polarization memory spectra. Black-Right-Pointing-Pointer Thermal annealing of porous anodic alumina can improve the process of excitation transfer.

  6. Rhodamine 6G hexachloridostannate(IV acetonitrile disolvate

    Directory of Open Access Journals (Sweden)

    Ramaiyer Venkatraman

    2008-01-01

    Full Text Available In the title compound, bis({6-ethylamino-10-[2-(methoxycarbonylphenyl]-2,7-dimethylxanthen-3-ylidene}ethanaminium hexachloridotin(IV acetonitrile disolvate, (C27H29N2O32[SnCl6]·2C2H3N, the octahedral SnCl62− anion lies on an inversion center. The xanthene ring system is essentially planar, with an average deviation of 0.020 Å, and the substituent benzene ring forms a dihedral angle of 85.89 (2° with it. The Sn—Cl distances are in the range 2.4237 (3–2.4454 (3 Å. There are N—H...Cl hydrogen bonds between SnCl62− ions and rhodamine 6G cations as well as π–π stacking interactions between rhodamine 6G cations (interplanar distance of 3.827 Å.

  7. Tunable cytotoxicity of rhodamine 6G via anion variations.

    Science.gov (United States)

    Magut, Paul K S; Das, Susmita; Fernand, Vivian E; Losso, Jack; McDonough, Karen; Naylor, Brittni M; Aggarwal, Sita; Warner, Isiah M

    2013-10-23

    Chemotherapeutic agents with low toxicity to normal tissues are a major goal in cancer research. In this regard, the therapeutic activities of cationic dyes, such as rhodamine 6G, toward cancer cells have been studied for decades with observed toxicities toward normal and cancer cells. Herein, we report rhodamine 6G-based organic salts with varying counteranions that are stable under physiological conditions, display excellent fluorescence photostability, and more importantly have tunable chemotherapeutic properties. Our in vitro studies indicate that the hydrophobic compounds of this series allow production of nanoparticles which are nontoxic to normal cells and toxic to cancer cells. Furthermore, the anions, in combination with cations such as sodium, were observed to be nontoxic to both normal and cancer cells. To the best of our knowledge, this is the first demonstration that both the cation and anion play an extremely important and cooperative role in the antitumor properties of these compounds.

  8. Rhodamine 6G impregnated porous silica: A photoluminescence study

    Energy Technology Data Exchange (ETDEWEB)

    Anedda, A. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Canada) (Italy); Carbonaro, C.M. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Canada) (Italy)]. E-mail: cm.carbonaro@dsf.unica.it; Clemente, F. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy); Corpino, R. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy); Ricci, P.C. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy); Rossini, S. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy)

    2005-12-15

    The optical properties of rhodamine 6G dye confined in porous silica are reported. Photoluminescence properties of embedded chromophores in mesoporous hosts can be affected by the surrounding matrices: shifts in emission spectra and variations of photoluminescence quantum yield are found as compared to dye solutions. Host-guest interactions are studied here by varying both SiO{sub 2} xerogels porosity and the dye concentration. Comparing samples obtained by impregnating matrices with 5.4 and 18.2 nm pores with solutions having concentrations in the rhodamine 6G high laser gain, matrices with 5.4 nm pores impregnated with a dye concentration of 5 x 10{sup -4} M are found to be the most stable and efficient in the examined range.

  9. Extended rhodamine photosensitizers for photodynamic therapy of cancer cells.

    Science.gov (United States)

    Davies, Kellie S; Linder, Michelle K; Kryman, Mark W; Detty, Michael R

    2016-09-01

    Extended thio- and selenorhodamines with a linear or angular fused benzo group were prepared. The absorption maxima for these compounds fell between 640 and 700nm. The extended rhodamines were evaluated for their potential as photosensitizers for photodynamic therapy in Colo-26 cells. These compounds were examined for their photophysical properties (absorption, fluorescence, and ability to generate singlet oxygen), for their dark and phototoxicity toward Colo-26 cells, and for their co-localization with mitochondrial-specific agents in Colo-26 and HUT-78 cells. The angular extended rhodamines were effective photosensitizers toward Colo-26 cells with 1.0Jcm(-2) laser light delivered at λmax±2nm with values of EC50 of (2.8±0.4)×10(-7)M for sulfur-containing analogue 6-S and (6.4±0.4)×10(-8)M for selenium-containing analogue 6-Se. The linear extended rhodamines were effective photosensitizers toward Colo-26 cells with 5 and 10Jcm(-2) of broad-band light (EC50's⩽2.4×10(-7)M). Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Fluorescence studies of Rhodamine 6G functionalized silicon oxide nanostructures

    International Nuclear Information System (INIS)

    Baumgaertel, Thomas; Borczyskowski, Christian von; Graaf, Harald

    2010-01-01

    Selective anchoring of optically active molecules on nanostructured surfaces is a promising step towards the creation of nanoscale devices with new functionalities. Recently we have demonstrated the electrostatic attachment of charged fluorescent molecules on silicon oxide nanostructures prepared by atomic force microscopy (AFM) nanolithography via local anodic oxidation (LAO) of dodecyl-terminated silicon. In this paper we report on our findings from a more detailed optical investigation of the bound dye Rhodamine 6G. High sensitivity optical wide field microscopy as well as confocal laser microscopy have been used to characterize the Rhodamine fluorescence emission. A highly interesting question concerns the interaction between an emitter close to a silicon surface because mechanisms such as energy transfer and fluorescence quenching will occur which are still not fully understood. Since the oxide thickness can be varied during preparation continuously from 1 to ∼ 5 nm, it is possible to investigate the fluorescence of the bound dye in close proximity to the underlying silicon. Using confocal laser microscopy we were also able to obtain optical spectra from the bound molecules. Together with the results from an analysis of their photochemical bleaching behaviour, we conjecture that some of the Rhodamine 6G molecules on the structure are interacting with the oxide, causing a spectral shift and differences in their photochemical properties.

  11. Spectroscopic insights on selfassembly and excited state interactions between rhodamine and phthalocyanine molecules.

    Science.gov (United States)

    Geng, Hao; Zhang, Xian-Fu

    2015-03-15

    The absorption and fluorescence spectra as well as fluorescence lifetimes of tetrasulfonated zinc phthalocyanine ZnPc(SO3Na)4 were measured in the absence and presence of four rhodamine dyes, Rhodamine B (RB), Ethyl rhodamine B (ERB), Rhodamine 6G (R6G), Rhodamine 110 (R110), and Pyronine B (PYB). The ground state complexes of phthalocyanine-(Rhodamine)2 were observed which exhibit new absorption bands. The binding constants are all very large (0.86×10(5)-0.22×10(8) M(-1)), suggesting rhodamine-phthalocyanine pairs are very good combinations for efficient selfassembly. Both the fluorescence intensity and the lifetime values of ZnPc(SO3Na)4 were decreased by the presence of rhodamines. The structural effect of rhodamines on selfassembly is significant. The ground state binding and dynamic quenching capability is PYB>R6G>ERB>RB>R110. The dynamic fluorescence quenching is due to the photoinduced electron transfer (PET). The PET rate constant is very large and in the order of 10(13) M(-1) s(-1), much greater than kf and kic (in the order of 10(8) M(-1) s(-1)), which means that the PET efficiency is almost 100%. Therefore the non-covalent Pc-rhodamine is a very good pair of donor/acceptor for potential efficient solar energy conversion. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Organic cation rhodamines for screening organic cation transporters in early stages of drug development.

    Science.gov (United States)

    Ugwu, Malachy C; Oli, Angus; Esimone, Charles O; Agu, Remigius U

    The aim of this study was to investigate the suitability of rhodamine-123, rhodamine-6G and rhodamine B as non-radioactive probes for characterizing organic cation transporters in respiratory cells. Fluorescent characteristics of the compounds were validated under standard in vitro drug transport conditions (buffers, pH, and light). Uptake/transport kinetics and intracellular accumulation of the compounds were investigated. Uptake/transport mechanisms were investigated by comparing the effect of pH, temperature, concentration, polarity, OCTs/OCTNs inhibitors/substrates, and metabolic inhibitors on the cationic dyes uptake in Calu-3 cells. Fluorescence stability and intensity of the compounds were altered by buffer composition, light, and pH. Uptake of the dyes was concentration-, temperature- and pH-dependent. OCTs/OCTNs inhibitors significantly reduced intracellular accumulation of the compounds. Whereas rhodamine-B uptake was sodium-dependent, pH had no effect on rhodamine-123 and rhodamine-6G uptake. Transport of the dyes across the cells was polarized: (AP→BL>BL→AP transport) and saturable: {V max =14.08±2.074, K m =1821±380.4 (rhodamine-B); V max =6.555±0.4106, K m =1353±130.4 (rhodamine-123) and V max =0.3056±0.01402, K m =702.9±60.97 (rhodamine-6G)}. The dyes were co-localized with MitoTracker®, the mitochondrial marker. Cationic rhodamines, especially rhodamine-B and rhodamine- 6G can be used as organic cation transporter substrates in respiratory cells. During such studies, buffer selection, pH and light exposure should be taken into consideration. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Fluorescence quenching of Rhodamine B base by two amines

    Science.gov (United States)

    Bakkialakshmi, S.; Selvarani, P.; Chenthamarai, S.

    2013-03-01

    Fluorescence quenching of Rhodamine B base (RhB) in DMF solution has been studied at different concentrations of the amine Triethyl amine (TEA) and n-butyl amine (NBA) at room temperature. It has been observed that the fluorescence intensity of RhB decrease with increase in the concentration of the TEA and NBA. It has been observed that the quenching due to amines proceeds via dynamic quenching process. The rate constants for the quenching process have been calculated using Stern-Volmer equation. Time resolved fluorescence study and 1H NMR spectral study have also been carried out and discussed.

  14. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects.

    Science.gov (United States)

    Zehentbauer, Florian M; Moretto, Claudia; Stephen, Ryan; Thevar, Thangavel; Gilchrist, John R; Pokrajac, Dubravka; Richard, Katherine L; Kiefer, Johannes

    2014-01-01

    Rhodamine 6G (R6G), also known as Rhodamine 590, is one of the most frequently used dyes for application in dye lasers and as a fluorescence tracer, e.g., in the area of environmental hydraulics. Knowing the spectroscopic characteristics of the optical emission is key to obtaining high conversion efficiency and measurement accuracy, respectively. In this work, solvent and concentration effects are studied. A series of eight different organic solvents (methanol, ethanol, n-propanol, iso-propanol, n-butanol, n-pentanol, acetone, and dimethyl sulfoxide (DMSO)) are investigated at constant dye concentration. Relatively small changes of the fluorescence spectrum are observed for the different solvents; the highest fluorescence intensity is observed for methanol and lowest for DMSO. The shortest peak wavelength is found in methanol (568 nm) and the longest in DMSO (579 nm). Concentration effects in aqueous R6G solutions are studied over the full concentration range from the solubility limit to highly dilute states. Changing the dye concentration provides tunability between ∼550 nm in the dilute case and ∼620 nm at high concentration, at which point the fluorescence spectrum indicates the formation of R6G aggregates. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. On different photodecomposition behaviors of rhodamine B on laponite and montmorillonite clay under visible light irradiation

    KAUST Repository

    Wang, Peng; Cheng, Mingming; Zhang, Zhonghai

    2013-01-01

    In this study, laponite and montmorillonite clays were found to be able to decompose rhodamine B upon visible light irradiation (λ>420nm). Very interestingly, it was found that rhodamine B on laponite underwent a stepwise N-deethylation and its

  16. Synthesis of a Far-Red Photoactivatable Silicon-Containing Rhodamine for Super-Resolution Microscopy.

    Science.gov (United States)

    Grimm, Jonathan B; Klein, Teresa; Kopek, Benjamin G; Shtengel, Gleb; Hess, Harald F; Sauer, Markus; Lavis, Luke D

    2016-01-26

    The rhodamine system is a flexible framework for building small-molecule fluorescent probes. Changing N-substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si-containing analogue of Q-rhodamine. This probe is the first example of a "caged" Si-rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red-shifted to allow multicolor imaging. The dye is a useful label for super-resolution imaging and constitutes a new scaffold for far-red fluorogenic molecules. © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

  17. Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy

    Science.gov (United States)

    Grimm, Jonathan B.; Klein, Teresa; Kopek, Benjamin G.; Shtengel, Gleb; Hess, Harald F.; Sauer, Markus

    2015-01-01

    Abstract The rhodamine system is a flexible framework for building small‐molecule fluorescent probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si‐containing analogue of Q‐rhodamine. This probe is the first example of a “caged” Si‐rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red‐shifted to allow multicolor imaging. The dye is a useful label for super‐resolution imaging and constitutes a new scaffold for far‐red fluorogenic molecules. PMID:26661345

  18. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells.

    Science.gov (United States)

    Tan, Dehong; Bai, Bing; Jiang, Donghua; Shi, Lin; Cheng, Shunchang; Tao, Dongbing; Ji, Shujuan

    2014-03-01

    The cytogenetic toxicity of rhodamine B on root tip cells of Allium cepa was investigated. A. cepa were cultured in water (negative control), 10 ppm methyl methanesulfonate (positive control), and three concentrations of rhodamine B (200, 100, and 50 ppm) for 7 days. Rhodamine B inhibited mitotic activity; increased nuclear anomalies, including micronuclei, nuclear buds, and bridged nuclei; and induced oxidative stress in A. cepa root tissues. Furthermore, a substantial amount of long nucleoplasmic bridges were entangled together, and some nuclei were simultaneously linked to several other nuclei and to nuclear buds with nucleoplasmic bridges in rhodamine B-treated cells. In conclusion, rhodamine B induced cytogenetic effects in A. cepa root tip cells, which suggests that the A. cepa root is an ideal model system for detecting cellular interactions.

  19. [Fluorescence Resonance Energy Transfer Detection of Cobalt Ions by Silver Triangular Nanoplates and Rhodamine 6G].

    Science.gov (United States)

    Zhang, Xiu-qing; Peng, Jun; Ling, Jian; Liu, Chao-juan; Cao, Qiu-e; Ding, Zhong-tao

    2015-04-01

    In the present paper, the authors studied fluorescence resonance energy transfer (FRET) phenomenon between silver triangular nanoplates and bovine serum albumin (BSA)/Rhodamine 6G fluorescence complex, and established a fluorescence method for the detection of cobalt ions. We found that when increasing the silver triangular nanoplates added to certain concentrations of fluorescent bovine serum albumin (BSA)/Rhodamine 6G complex, the fluorescence of Rhodamine 6G would be quenched up to 80% due to the FRET between the quencher and donor. However, in the presence of cobalt ions, the disassociation of the fluorescent complex from silver triangular nanoplates occurred and the fluorescence of the Rhodamine 6G recovered. The recovery of fluorescence intensity rate (I/I0) has a good relationship with the cobalt ion concentration (cCO2+) added. Thus, the authors developed a fluorescence method for the detection of cobalt ions based on the FRET of silver triangular nanoplates and Rhodamine 6G.

  20. IDENTIFIKASI RHODAMIN B PADA SAUS TOMAT YANG BEREDAR DI PASAR PAGI SAMARINDA

    Directory of Open Access Journals (Sweden)

    Eka Siswanto Syamsul

    2018-03-01

    Full Text Available Rhodamin B is a green powder synthetic dye used as a textile dye and is often used to color a food product, especially tomato sauce. Tomato sauce sampled in Samarinda Morning Market for 5 samples. This research used color reaction test and KLT method. The result of the research using color reaction test showed that the sample did not contain rhodamine synthetic dye B. The result of identification with thin layer chromatography obtained Rf value of A sample 0,46, sample B 0,46, sample C 0,4, sample D 0,5 and sample E 0.78, the value of Rf standard rhodamin B 0.8. From the value of Rf, the sample E contains rhodamine B because the color of the sample spots and rhodamin B are the same pink and the difference of Rf value is in the range ± 0.02 of the standard Rf.

  1. Penetration pattern of rhodamine dyes into enamel and dentin: confocal laser microscopy observation.

    Science.gov (United States)

    Kwon, S R; Wertz, P W; Li, Y; Chan, D C N

    2012-02-01

    Enamel and dentin are susceptible to extrinsic and intrinsic stains. The purposes of this study were to determine the penetration pattern of Rhodamine B and dextran-conjugated Rhodamine B into the enamel and dentin as observed by confocal laser microscopy and to relate it to the penetration pattern of hydrogen peroxide commonly used as an active ingredient in tooth-whitening agents and high-molecular-weight staining molecules. Eighteen recently extracted human maxillary anterior teeth were used. Teeth were cleaned and painted with nail varnish except for the crown area above the cemento-enamel junction (CEJ). The painted teeth were then immersed in Rhodamine B and dextran-conjugated Rhodamine B (70 000 MW) for 4, 7, 10 and 15 days. Teeth were sliced to 3 mm thickness in transverse plane and mounted on a glass slide just prior to observation with confocal laser microscopy. Rhodamine B and dextran-conjugated Rhodamine B readily penetrated into the enamel and dentin when exposed for 4 and 7 days, respectively. Rhodamine B penetrated along the interprismatic spaces of the enamel into the dentin. The penetration was accentuated in sections with existing crack lines in the enamel. Rhodamine B was readily absorbed into the dentinal tubules at the dentino-enamel junction and continued to penetrate through the dentin via the dentinal tubules into the pre-dentin. Within the limitations of this study, it is concluded that Rhodamine B and dextran-conjugated Rhodamine B when applied to the external surface of the tooth readily penetrate into the enamel and dentin via the interprismatic spaces in the enamel and dentinal tubules in the dentin, suggesting that stain molecules and bleaching agents possibly exhibit similar penetration pathways. © 2011 The Authors. ICS © 2011 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

  2. Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining

    Science.gov (United States)

    Zaitsev, Sergei Yu.; Shaposhnikov, Mikhail N.; Solovyeva, Daria O.; Solovyeva, Valeria V.; Rizvanov, Albert A.

    2014-01-01

    Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (“Chitosan-PFD”) and histone H1 (“Histone H1.3-PFD”). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (“caged” dyes) for microscopic probing of biological objects. Thus, the synthesized “Chitosan-PFD” and “Histone H1-PFD” have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy. PMID:25383365

  3. Determination of Cerium (IV) Using Rhodamine 6G Fluorescence Quenching

    Science.gov (United States)

    Zhao, Zh.; Sheng, L.; Su, B.; Tao, C.; Jing, W.

    2017-11-01

    The interaction between rhodamine 6G (Rh6G) and cerium sulfate was studied by the fluorescence quenching method. In a sulfuric acid medium, the interaction of Ce(IV) with Rh6G results in Rh6G fluorescence quenching. The maximum excitation wavelength (λex) and the maximum emission wavelength (λem) are 530 nm and 555 nm, respectively. A good linearity between the relative fl uorescence intensity (ΔF) and Ce(IV) was observed in the range 0.12-1.08 μg/mL. The detection limit was 1.4 × 10-3 μg/mL. The optimum reaction conditions, influencing factors, and effect of coexisting substances were investigated in the experiment. We found that the concentration of Rh6G was 3.2 × 10-6 mol/L, and the fl uorescence intensity was maximum.

  4. PENURUNAN KADAR RHODAMIN B DALAM AIR LIMBAH DENGAN BIOFILTRASI SISTEM TANAMAN

    Directory of Open Access Journals (Sweden)

    K. Yogi Purnamawati

    2016-01-01

    Full Text Available The textile industry is growing rapidly and as the result it’s producing waste that can harm the environment. One of which is rhodamine B. Rhodamine B is a synthetics dyes that have a form crystalline which an organic base containing amino groups, so it is difficult to degrade naturally by microorganism. Biofiltration system method is one of many ways in handling wastewater. Layered filtration unit of sand and rocks combine with the adsorption of plant and decomposition by microorganisms in rhizosphere so that wastewater can be reused. The aim of this study determined effectiveness and capacity of biofiltration system vegetation in reducing concentrate of rhodamine-B, total dissolved solid (TDS, total suspended solid (TSS and the pH in wastewater. The result showed that biofiltration effectiveness in reducing rhodamine B, TDS and TSS concetrate were 51,70%; 47,60%; 50,44% while decreasing and stabilization of pH obtained at 30 hours treatment time with pH value is 7,5. Capacity of biofiltration system vegetation with volume 0,06 m3 can reduced rhodamine B, TDS and TSS by 0,2256 ppm; 278,0237 ppm and 9,4978 ppm respectively, while the optimum detention time of wastewater in the biosystem for reducing rhodamine B was 30 hours and for TSS and TDS was 36 hours. It can be concluded that biofiltration system vegetation was able to reduce rhodamine B, TDS, TSS and pH of wastewater

  5. Influence of the UV Radiation on Rhodamine WT Fluorescence in Water Samples

    OpenAIRE

    , L. Kola; , P. Lazo

    2016-01-01

    The şuorescence ability of Rhodamine WT enables its using as artiŞcial tracer in the water system studies. The problem is dealt with in relation to applying Rhodamine WT (RhWT) to trace and determine water movements within the karstic system and underground waters. Rhodamine WT has been used as an artiŞcial tracer for the Şrst times in our country on Mali me Gropa system study (2002). UV radiation may induce photochemical decomposition of the dye which can cause large measurement errors on me...

  6. A dansyl-rhodamine ratiometric fluorescent probe for Hg2+ based on FRET mechanism.

    Science.gov (United States)

    Xie, Puhui; Guo, Fengqi; Wang, Lingyu; Yang, Sen; Yao, Denghui; Yang, Guoyu

    2015-03-01

    Based on resonance energy transfer (FRET) from dansyl to rhodamine 101, a new fluorescent probe (compound 1) containing rhodamine 101 and a dansyl unit was synthesized for detecting Hg(2+) through ratiometric sensing in DMSO aqueous solutions. This probe shows a fast, reversible and selective response toward Hg(2+) in a wide pH range. Hg(2+) induced ring-opening reactions of the spirolactam rhodamine moiety of 1, leading to the formation of fluorescent derivatives that can serve as the FRET acceptors. Very large stokes shift (220 nm) was observed in this case. About 97-fold increase in fluorescence intensity ratio was observed upon its binding with Hg(2+).

  7. Stimulation of mitochondrial respiration induced by laser irradiation in the presence of rhodamine dyes

    International Nuclear Information System (INIS)

    Krasnikov, B.F.; Zorov, D.B.

    1996-01-01

    The effect of micromolar concentration of rhodamine 123 (methylrhodamine) and ethyl and amyl esters of unsubstituted rhodamine on oxygen consumption by rat liver mitochondria was studied under irradiation by an argon laser (488 and 514 nm). Irradiation of mitochondria in the presence of rhodamine stimulates their respiration. Light-induced stimulation of respiration is not inhibited by free radical scavenger ionol and by inhibitor of the permeability transition pore cyclosporine A. Stimulation of respiration by moderate doses of radiation is reversed in the dark. Increase in radiation dose resulted in only partial reversal of stimulated respiration in the dark. Rhodamine efficacy in stimulation of mitochondrial respiration depends on its structure (amyl > ethyl > methylrhodamine). 22 refs.; 4 figs

  8. Fabrication and Photostability of Rhodamine-6G Gold Nanoparticle Doped Polymer Optical Fiber

    International Nuclear Information System (INIS)

    Sebastian, Suneetha; Ajina, C; Vallabhan, C. P. G; Nampoori, V. P. N.; Radhakrishnan, P.; Kailasnath, M.

    2013-01-01

    We report on fabrication of a rhodamine-6G-gold-nanoparticle doped polymer optical fiber. The gold nanoparticle is synthesized directly into the monomer solution of the polymer using laser ablation synthesis in liquid. The size of the particle is found from the transmission electron microscopy. Rhodamine-6G is then mixed with the nanoparticle-monomer solution and optical characterization of the solution is investigated. It is found that there is a pronounced quenching of fluorescence of rhodamine 6G due to fluorescence resonance energy transfer. The monomer solution containing rhodamine 6G and gold nanoparticles is now made into a cylindrical rod and drawn into a polymer optical fiber. Further, the photostability is calculated with respect to the pure dye doped polymer optical fiber

  9. Rhodamine 800 as reference substance for fluorescence quantum yield measurements in deep red emission range

    Energy Technology Data Exchange (ETDEWEB)

    Alessi, A., E-mail: andrea.alessi@eni.com [Centro Ricerche per le Energie non Convenzionali, Istituto eni Donegani, e.n.i. S.p.A., Via G. Fauser 4, 28100 Novara (Italy); Salvalaggio, M. [Centro Ricerche per le Energie non Convenzionali, Istituto eni Donegani, e.n.i. S.p.A., Via G. Fauser 4, 28100 Novara (Italy); Ruzzon, G. [HORIBA Jobin Yvon Srl, Via Cesare Pavese 35/AB, 20090 Opera Milano (Italy)

    2013-02-15

    The determination of fluorescence quantum yields ({Phi}{sub f}) of deep red dyes emitting at 635-900 nm is difficult due to lack of suitable standards. In this work, we propose a commercial dye, rhodamine 800 (Rho800), as reference standard which belongs to the family of xanthenes. The quantum yield of rhodamine 800 in absolute ethanol has been studied using a relative method with cresyl violet (CV) and rhodamine 101 (Rho101) as references, and an absolute fluorometric method by integrating sphere measurements. - Highlights: Black-Right-Pointing-Pointer A red emitting dye Rhodamine 800 was electronic spectroscopy characterized. Black-Right-Pointing-Pointer Its fluorescence quantum yield was studied using a relative and an absolute method. Black-Right-Pointing-Pointer The values found are greater than the values currently known in the literature.

  10. Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent

    International Nuclear Information System (INIS)

    Heinrich, Tobias K.; Gottumukkala, Vijay; Snay, Erin; Dunning, Patricia; Fahey, Frederic H.; Ted Treves, S.; Packard, Alan B.

    2010-01-01

    There is considerable interest in developing an 18 F-labeled PET myocardial perfusion agent. Rhodamine dyes share several properties with 99m Tc-MIBI, the most commonly used single-photon myocardial perfusion agent, suggesting that an 18 F-labeled rhodamine dye might prove useful for this application. In addition to being lipophilic cations, like 99m Tc-MIBI, rhodamine dyes are known to accumulate in the myocardium and are substrates for Pgp, the protein implicated in MDR1 multidrug resistance. As the first step in determining whether 18 F-labeled rhodamines might be useful as myocardial perfusion agents for PET, our objective was to develop synthetic methods for preparing the 18 F-labeled compounds so that they could be evaluated in vivo. Rhodamine B was chosen as the prototype compound for development of the synthesis because the ethyl substituents on the amine moieties of rhodamine B protect them from side reactions, thus eliminating the need to include (and subsequently remove) protecting groups. The 2'-[ 18 F]fluoroethyl ester of rhodamine B was synthesized by heating rhodamine B lactone with [ 18 F]fluoroethyltosylate in acetonitrile at 165 deg. C for 30 min using [ 18 F]fluoroethyl tosylate, which was prepared by the reaction of ethyleneglycol ditosylate with Kryptofix 2.2.2, K 2 CO 3 , and [ 18 F]NaF in acetonitrile for 10 min at 90 deg. C. The product was purified by semi-preparative HPLC to produce the 2'-[ 18 F]fluoroethylester in >97% radiochemical purity with a specific activity of 1.3 GBq/μmol, an isolated decay corrected yield of 35%, and a total synthesis time of 90 min.

  11. Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent

    Science.gov (United States)

    Heinrich, Tobias K.; Gottumukkala, Vijay; Snay, Erin; Dunning, Patricia; Fahey, Frederic H; Treves, S. Ted; Packard, Alan B.

    2009-01-01

    There is considerable interest in developing an 18F-labeled PET myocardial perfusion agent. Rhodamine dyes share several properties with 99mTc-MIBI, the most commonly used single-photon myocardial perfusion agent, suggesting that an 18F-labeled rhodamine dye might prove useful for this application. In addition to being lipophilic cations, like 99mTc-MIBI, rhodamine dyes are known to accumulate in the myocardium and are substrates for Pgp, the protein implicated in MDR1 multidrug resistance. As the first step in determining whether 18F-labeled rhodamines might be useful as myocardial perfusion agents for PET, our objective was to develop synthetic methods for preparing the 18F-labeled compounds so that they could be evaluated in vivo. Rhodamine B was chosen as the prototype compound for development of the synthesis because the ethyl substituents on the amine moieties of rhodamine B protect them from side reactions, thus eliminating the need to include (and subsequently remove) protecting groups. The 2′-[18F]fluoroethyl ester of rhodamine B was synthesized by heating rhodamine B lactone with [18F]fluoroethyltosylate in acetonitrile at 165°C for 30 min.using [18F]fluoroethyl tosylate, which was prepared by the reaction of ethyleneglycol ditosylate with Kryptofix 2.2.2, K2CO3, and [18F]NaF in acetonitrile for 10 min. at 90°C. The product was purified by semi-preparative HPLC to produce the 2′-[18F]-fluoroethylester in >97% radiochemical purity with a specific activity of 1.3 GBq/μmol, an isolated decay corrected yield of 35%, and a total synthesis time of 90 min. PMID:19783150

  12. Turn-on fluorescent chemosensor for Hg2+ based on multivalent rhodamine ligands

    NARCIS (Netherlands)

    Wang, X.; Iqbal, M.; Huskens, Jurriaan; Verboom, Willem

    2012-01-01

    Rhodamine-based fluorescent chemosensors 1 and 2 exhibit selective fluorescence enhancement to Fe3+ and Hg2+ over other metal ions at 580 nm in CH3CN/H2O (3/1, v/v) solution. Bis(rhodamine) chemosensor 1, under optimized conditions (CH3CN/HEPES buffer (0.02 M, pH = 7.0) (95/5, v/v)), shows a high

  13. A NOVEL RHODAMINE-BASED FLUORESCENCE CHEMOSENSOR CONTAINING POLYETHER FOR MERCURY (II IONS IN AQUEOUS SOLUTION

    Directory of Open Access Journals (Sweden)

    Wenqi Du

    Full Text Available A novel rhodamine-based Hg2+ chemosensor P2 containing polyether was readily synthesized and investigated, which displayed high selectivity and sensitivity for Hg2+. Because of good water-solubility of polyther, the rhodamine-based chemosensor containing polyether can be used in aqueous solution. The sensor responded rapidly to Hg2+ in pure water solutions with a 1:1 stoichiometry. Meanwhile, it indicated excellent adaptability and also the responsiveness.

  14. Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Heinrich, Tobias K.; Gottumukkala, Vijay [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States); Snay, Erin; Dunning, Patricia [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Fahey, Frederic H.; Ted Treves, S. [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States); Packard, Alan B. [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States)], E-mail: alan.packard@childrens.harvard.edu

    2010-01-15

    There is considerable interest in developing an {sup 18}F-labeled PET myocardial perfusion agent. Rhodamine dyes share several properties with {sup 99m}Tc-MIBI, the most commonly used single-photon myocardial perfusion agent, suggesting that an {sup 18}F-labeled rhodamine dye might prove useful for this application. In addition to being lipophilic cations, like {sup 99m}Tc-MIBI, rhodamine dyes are known to accumulate in the myocardium and are substrates for Pgp, the protein implicated in MDR1 multidrug resistance. As the first step in determining whether {sup 18}F-labeled rhodamines might be useful as myocardial perfusion agents for PET, our objective was to develop synthetic methods for preparing the {sup 18}F-labeled compounds so that they could be evaluated in vivo. Rhodamine B was chosen as the prototype compound for development of the synthesis because the ethyl substituents on the amine moieties of rhodamine B protect them from side reactions, thus eliminating the need to include (and subsequently remove) protecting groups. The 2'-[{sup 18}F]fluoroethyl ester of rhodamine B was synthesized by heating rhodamine B lactone with [{sup 18}F]fluoroethyltosylate in acetonitrile at 165 deg. C for 30 min using [{sup 18}F]fluoroethyl tosylate, which was prepared by the reaction of ethyleneglycol ditosylate with Kryptofix 2.2.2, K{sub 2}CO{sub 3}, and [{sup 18}F]NaF in acetonitrile for 10 min at 90 deg. C. The product was purified by semi-preparative HPLC to produce the 2'-[{sup 18}F]fluoroethylester in >97% radiochemical purity with a specific activity of 1.3 GBq/{mu}mol, an isolated decay corrected yield of 35%, and a total synthesis time of 90 min.

  15. Use of scandium ionic associates with salicylic- or 2-phenylquinoline-4-carboxylic acid and rhodamine C

    Energy Technology Data Exchange (ETDEWEB)

    Kononenko, L.I.; Bel' tyukova, S V; Drobyazko, V N; Poluehktov, N S [AN Ukrainskoj SSR, Kiev. Inst. Obshchej i Neorganicheskoj Khimii; AN Ukrainskoj SSR, Odessa. Inst. Obshchej i Neorganicheskoj Khimii)

    1975-09-01

    With salicylic or 2-phenylquinoline-4-carboxylic acid and rhodamine C scandium forms ion associations whose benzene solutions are capable of luminescence. Optimum conditions for the formation of complexes and the composition of the complex with the ratio of Sc:acid:rhodamine C = 1:2:1 are established. A possibility of luminescence determination of scandium in the presence of rare earths is shown.

  16. Preparation of Rhodamine B Fluorescent Poly(methacrylic acid) Coated Gelatin Nanoparticles

    OpenAIRE

    Gan, Zhenhai; Ju, Jianhui; Zhang, Ting; Wu, Daocheng

    2011-01-01

    Poly(methacrylic acid) (PMAA)-coated gelatin nanoparticles encapsulated with fluorescent dye rhodamine B were prepared by the coacervation method with the aim to retard the release of rhodamine B from the gelatin matrix. With sodium sulfate as coacervation reagent for gelatin, a kind of biopolymer with excellent biocompatibility, the formed gelatin nanoparticles were cross-linked by formaldehyde followed by the polymerization of methacrylic acid coating. The fluorescent poly(methacrylic acid)...

  17. Synthesis of an amphiphilic rhodamine derivative and characterization of its solution and thin film properties

    International Nuclear Information System (INIS)

    Aviv, Hagit; Harazi, Sivan; Schiff, Dillon; Ramon, Yoni; Tischler, Yaakov R.

    2014-01-01

    Here we present characterization of solution and thin film properties of Lissamine rhodamine B sulfonyl didodecyl amine (LRSD), an amphiphilic derivative of rhodamine. LRSD was synthesized by functionalizing Lissamine rhodamine B sulfonyl chloride (LRSC) with didodecylamine via a straightforward sulfonylation reaction. LRSD's long alkane chains make it highly soluble in chloroform, with a marked increase in brightness compared to the starting material. LRSD is shown to form well-defined robust micelles in water, without the addition of a co-surfactant and stable monolayers at the air–water interface. The greater lipophilicity of LRSD also enables doping into non-polar polymeric host matrices such as polystyrene with less aggregation and hence higher fluorescence quantum yield than LRSC or even rhodamine B. The monolayers of LRSD were prepared via Langmuir–Blodgett deposition and showed shifts in the photoluminescence peak from 575 nm to 595 nm, as the surface pressure is varied from 3 mN/m to 11 mN/m. - Highlights: • Lissamine rhodamine B sulfonyl didodecyl amine (LRSD) is soluble in chloroform. • LRSD shows robust quantum yield in solution and as a dopant in thin film. • LRSD is an amphiphilic rhodamine dye that forms compact fluorescent micelles. • LRSD forms a stable isotherm when spread at the air–water interface

  18. Electrochemical Degradation of Rhodamine B over Ti/SnO2-Sb Electrode.

    Science.gov (United States)

    Maharana, Dusmant; Niu, Junfeng; Gao, Ding; Xu, Zesheng; Shi, Jianghong

    2015-04-01

    Electrochemical degradation of rhodamine B (C28H31ClN2O3) over Ti/SnO2-Sb anode was investigated in a rectangular cell. The degradation reaction follows pseudo-first-order kinetics. The degradation efficiency of rhodamine B attained >90.0% after 20 minutes of electrolysis at initial concentrations of 5 to 200 mg/L at a constant current density of 20 mA/cm2 with a 10 mmol/L Na2SO4 supporting electrolyte solution. Rhodamine B (50 mg/L) degradation and total organic carbon (TOC) removal ratio achieved 99.9 and 86.7%, respectively, at the optimal conditions after 30 minutes of electrolysis. The results showed that the energy efficiency of rhodamine B (50 mg/L) degradation at the optimal current densities from 2 to 30 mA/cm2 were 23.2 to 84.6 Wh/L, whereas the electrolysis time for 90% degradation of rhodamine B with Ti/SnO2-Sb anode was 36.6 and 7.3 minutes, respectively. The electrochemical method can be an advisable option for the treatment of dyes such as rhodamine B in wastewater.

  19. A Photostable Silicon Rhodamine Platform for Optical Voltage Sensing

    Science.gov (United States)

    Huang, Yi-Lin; Walker, Alison S.; Miller, Evan W.

    2015-01-01

    This paper describes the design and synthesis of a photostable, far-red to near-infrared (NIR) platform for optical voltage sensing. We developed a new, sulfonated silicon rhodamine fluorophore and integrated it with a phenylenevinylene molecular wire to create a Berkeley Red Sensor of Transmembrane potential, or BeRST 1 (“burst”). BeRST 1 is the first member of a class of farred to NIR voltage sensitive dyes that make use of a photoinduced electron transfer (PeT) trigger for optical interrogation of membrane voltage. We show that BeRST 1 displays bright, membrane-localized fluorescence in living cells, high photostability, and excellent voltage sensitivity in neurons. Depolarization of the plasma membrane results in rapid fluorescence increases (24% ΔF/F per 100 mV). BeRST 1 can be used in conjunction with fluorescent stains for organelles, Ca2+ indicators, and voltage-sensitive fluorescent proteins. In addition, the red-shifted spectral profile of BeRST 1, relative to commonly employed optogenetic actuators like ChannelRhodopsin2 (ChR2), which require blue light, enables optical electrophysiology in neurons. The high speed, sensitivity, photostability and long-wavelength fluorescence profiles of BeRST 1 make it a useful platform for the non-invasive, optical dissection of neuronal activity. PMID:26237573

  20. Rhodamine/Nanodiamond as a System Model for Drug Carrier.

    Science.gov (United States)

    Reina, G; Orlanducci, S; Cairone, C; Tamburri, E; Lenti, S; Cianchetta, I; Rossi, M; Terranova, M L

    2015-02-01

    In this paper we present some strategies that are being developed in our labs towards enabling nanodiamond-based applications for drug delivery. Rhodamine B (RhB) has been choosen as model molecule to study the loading of nanodiamonds with active moieties and the conditions for their controlled release. In order to test the chemical/physical interactions between functionalized detonation nanodiamond (DND) and complex molecules, we prepared and tested different RhB@DND systems, with RhB adsorbed or linked by ionic bonding to the DND surface. The chemical state of the DND surfaces before conjugation with the RhB molecules, and the chemical features of the DND-RhB interactions have been deeply analysed by coupling DND with Au nanoparticles and taking advantage of surface enhanced Raman spectroscopy SERS. The effects due to temperature and pH variations on the process of RhB release from the DND carrier have been also investigated. The amounts of released molecules are consistent with those required for effective drug action in conventional therapeutic applications, and this makes the DND promising nanostructured cargos for drug delivery applications.

  1. On different photodecomposition behaviors of rhodamine B on laponite and montmorillonite clay under visible light irradiation

    KAUST Repository

    Wang, Peng

    2013-12-11

    In this study, laponite and montmorillonite clays were found to be able to decompose rhodamine B upon visible light irradiation (λ>420nm). Very interestingly, it was found that rhodamine B on laponite underwent a stepwise N-deethylation and its decomposition was terminated once rhodamine 110, as a decomposition product, was formed, whereas the same phenomenon was not observed for rhodamine B on montmorillonite, whose decomposition involved chromophore destruction. Mechanistic study revealed that the different photodecomposition behaviors of rhodamine B on laponite and montmorillonite were attributed to the oxidation by different reactive oxygen species, with laponite involving HO2/O2- while montmorillonite involving OH. It was also found that the degradation pathway of rhodamine B on laponite switched from N-deethylation to chromophore destruction when solution pH was changed from 7.0 to 3.0, which was attributed to a much higher fraction of HO2 relative to O2- under pH 3.0 than under pH 7.0. Based on the results, a mechanism of rhodamine dye decomposition on clay under visible light was proposed, involving the clay as an electron acceptor, electron relay between the adsorbed dye molecules and oxygen molecules, and subsequent reactions between the generated dye radical cations and different reactive oxygen species. The results of this study shed light on how to best utilize visible light for organic pollutant degradation on clays within engineered treatment systems as well as on many of naturally occurring pollutant degradation processes in soils and air involving clay. © 2013 King Saud University.

  2. On the use of rhodamine WT for the characterization of stream hydrodynamics and transient storage

    Science.gov (United States)

    Runkel, Robert L.

    2015-01-01

    Recent advances in fluorometry have led to increased use of rhodamine WT as a tracer in streams and rivers. In light of this increased use, a review of the dye's behavior in freshwater systems is presented. Studies in the groundwater literature indicate that rhodamine WT is transported nonconservatively, with sorption removing substantial amounts of tracer mass. Column studies document a two-step breakthrough curve in which two structural isomers are chromatographically separated. Although the potential for nonconservative transport is acknowledged in the surface water literature, many studies assume that sorptive losses will not affect the characterization of physical transport processes. A literature review and modeling analysis indicates that this assumption is valid for quantification of physical properties that are based on the bulk of the tracer mass (traveltime), and invalid for the characterization of processes represented by the tracer tail (transient storage attributable to hyporheic exchange). Rhodamine WT should be considered nonconservative in the hyporheic zone due to nonconservative behavior demonstrated for similar conditions in groundwater. As such, rhodamine WT should not be used as a quantitative tracer in hyporheic zone investigations, including the study of long flow paths and the development of models describing hyporheic zone processes. Rhodamine WT may be used to qualitatively characterize storage in large systems, where there are few practical alternatives. Qualitative investigations should rely on early portions of the tracer profile, making use of the temporal resolution afforded by in situ fluorometry, while discarding later parts of the tracer profile that are adversely affected by sorption.

  3. Photoionization of rhodamine dyes adsorbed at the aqueous solution surfaces investigated by synchrotron radiation

    International Nuclear Information System (INIS)

    Seno, Koichiro; Ishioka, Toshio; Harata, Akira; Hatano, Yoshihiko

    2002-01-01

    Photoionization spectroscopy using synchrotron radiation as a photon source was applied to the aqueous solution surfaces of rhodamine B (RhB), rhodamine 6G (Rh6G), and rhodamine 101 (Rh101) with their concentration of 0-100 μmol dm -3 . Synchrotron radiation was irradiated upon the solution surface between two electrodes in the photon energy range of 4-7 eV, and photoionization current was measured by a pico-ammeter. The photocurrent for each of the rhodamine aqueous solution surface showed an increase with the photon energy above a threshold photon energy. The photoionization threshold of RhB at the aqueous solution surface was 5.6 eV, which was smaller than that in the gas phase. The intensity of photoionization current of Rh6G was smaller than that of RhB or Rh101. Photoionization processes and the state of Rhodamine dye molecules at the aqueous solution surface were discussed in connection with results of surface tension measurements. (author)

  4. Extraction/fluorometric determination of uranium with rhodamine B and 2-thenoyltrifluoroacetone

    International Nuclear Information System (INIS)

    Naganuma, Takeshi; Tuzuki, Satoshi; Jin, Jiye.

    1990-01-01

    A method for the fluorometric determination of uranium, based on a solvent extraction of an ion associate formed between Rhodamine B cation and 2-thenoyltrifluoroacetone(TTA)-metal chelate anion with benzene, is described. The procedure for the construction of the calibration curve is as follows. Varying amounts of uranium standard solution and 2 ml of 2 M ammonium acetate buffer solution are mixed. The solution is adjusted at pH 6.0 and 10 ml of 0.3 % Rhodamine B are added. It is then shaken for 5 min with 10 ml of 0.01 M TTA benzene solution. The fluoresence of the organic phase is measured at 545 nm (excitation) and 570 nm (emission) for uranyl-TTA-Rhodamine B complex. The fluorescence intensity is linearly related to the concentration of uranyl ion in the range of 0.05 ppm ot 1 ppm. (author)

  5. A turn-on fluorescent rhodamine-acyl hydrazide for selective detection of Cu"2"+ ions

    International Nuclear Information System (INIS)

    Yoon, Jung Won; Jeong, Hyuk; Lee, Min Hee

    2017-01-01

    We present a rhodamine-based probe that can detect Cu"2"+ ions via Cu"2"+-promoted hydrolysis in aqueous solutions. In solution, the probe is non-fluorescent and colorless, but gives a strong fluorescence at 586 nm and shows a color change to pink on the addition of Cu"2"+ ions. We demonstrate that the rhodamine-based probe undergoes Cu"2"+-promoted hydrolysis with a moderate reaction time (within 3 min) and provides a fluorescence off–on change, even in the presence of other competitive metal ions. The rhodamine-based probe shows a linear correlation between increasing fluorescence at 586 nm and the Cu"2"+ concentration, and can detect Cu"2"+ at nanomolar levels (10 nM) in CH_3CN:H_2O (v/v, 90:10)

  6. Rhodamine B conjugates of triterpenoic acids are cytotoxic mitocans even at nanomolar concentrations.

    Science.gov (United States)

    Sommerwerk, Sven; Heller, Lucie; Kerzig, Christoph; Kramell, Annemarie E; Csuk, René

    2017-02-15

    Triterpenoic acids 1-6 exhibited very low or no cytotoxicity at all, but their corresponding 2,3-di-O-acetyl-piperazinyl amides 13-18 showed low EC 50 values for several human tumor cell lines. Their cytotoxicity, however, was also high for the non-malignant mouse fibroblasts NIH 3T3. A significant improvement was achieved by preparing the rhodamine B derivatives 19-24. While rhodamine B is not cytotoxic (up to a concentration of 30μM - cut-off of the assay), the triterpenoid piperazine-spacered rhodamine B derivatives were cytotoxic in nano-molar concentration. Compound 24 (a diacetylated maslinic acid derivative) was most toxic for several human tumor cell lines but less toxic for mouse fibroblasts NIH 3T3. Staining and double-staining experiments revealed 24 to act as a mitocan. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  7. A rhodamine B-based fluorescent sensor toward highly selective mercury (II) ions detection.

    Science.gov (United States)

    Jiao, Yang; Zhang, Lei; Zhou, Peng

    2016-04-01

    This work presented the design, syntheses and photophysical properties of a rhodamine B-based fluorescence probe, which exhibited a sensitive and selective recognition towards mercury (II). The chemosensor RA (Rhodamine- amide- derivative) contained a 5-aminoisophthalic acid diethyl ester and a rhodamine group, and the property of spirolactone of this chemosensor RA was detected by X-ray crystal structure analyses. Chemosensor RA afforded turn-on fluorescence enhancement and displayed high brightness for Hg(2+), which leaded to the opening of the spirolactone ring and consequently caused the appearance of strong absorption at visible range, moreover, the obvious and characteristic color changed from colorless to pink was observed. We envisioned that the chemosensor RA exhibited a considerable specificity with two mercury (II) ions which was attributed to the open of spirolactone over other interference metal ions. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Rhodamine dyes as potential agents for photochemotherapy of cancer in human bladder carcinoma cells

    International Nuclear Information System (INIS)

    Shea, C.R.; Chen, N.; Wimberly, J.; Hasan, T.

    1989-01-01

    The phototoxicity in vitro of rhodamine 123 and tetrabromo rhodamine 123 (TBR) was compared, in order to assess their photochemotherapeutic potential. Exposure to 514.5-nm radiation from an argon ion laser caused phototoxicity in MGH-U1 bladder carcinoma cells previously treated with either dye at 10 microM for 30 min. As assessed by colony formation and cellular morphology, TBR was markedly more phototoxic than rhodamine 123, reflecting increased intersystem crossing of TBR to the triplet manifold via spin-orbital coupling induced by the heavy bromine atoms. Photoreactions of TBR very efficiently generated singlet oxygen ( 1 O 2 ) in solution; furthermore, irradiation of TBR-treated cells was significantly more toxic when performed in the presence of deuterium oxide, an enhancer of damage caused by 1 O 2 . Retention of fluorescence in TBR-treated cells was enhanced by irradiation, indicating that a stable photoproduct may be formed in reaction with cellular components

  9. Preparation of a magnetic molecularly imprinted polymer for selective recognition of rhodamine B

    International Nuclear Information System (INIS)

    Liu, Xiuying; Yu, Dan; Yu, Yingchao; Ji, Shujuan

    2014-01-01

    Graphical abstract: A novel material based on the use of magnetic Fe3O4 nanoparticles coated with MMIP for preconcentration and determination of RhB in real samples prior to fluorospectrophotometry was developed. - Highlights: • A novel rhodamine B magnetic molecularly imprinted polymer by using Fe 3 O 4 magnetite as the magnetically susceptible component was synthesized. • The MMIP had rapid adsorption and high selectivity towards rhodamine B. • Rhodamine B can be extracted selectively by MMIP from real samples. • The method provides the advantages of short analysis time and high sensitivity. - Abstract: A novel magnetic molecularly imprinted polymer (MMIP) was developed as an adsorbent to selectively remove rhodamine B from real samples. The polymer was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermo-gravimetric analysis. Static adsorption, kinetic adsorption, and selective recognition experiments were also performed to investigate the specific adsorption equilibrium, kinetics, and selective recognition ability of the MMIP. The MMIPs had outstanding thermal stability, large adsorption capacity, and high competitive selectivity. When they were used as dispersed solid-phase extraction adsorbents in real samples, rhodamine B recovery was 79.97–81.88% and 75.56–79.74% in intra-day and inter-day reproducibility experiments with relative standard deviations lower than 2.62% and 4.28%, respectively. Extraction was optimized for yield and efficiency. Precision, accuracy, and linear working range were determined under optimal experimental conditions. The limits of detection and quantification were 1.05 and 3.49 μg L −1 , respectively. These results suggest MMIPs may be used for determination of rhodamine B in real samples

  10. Indicating pressure and environmental effects by means of the spectral shift with rhodamine B and fluorescein

    Directory of Open Access Journals (Sweden)

    R. M. Johann

    2015-07-01

    Full Text Available Fluorescence absorption and emission wavelengths can be influenced by environmental conditions, such as pressure, temperature and concentration. Here those effects are explored with an emphasis on determining the potential of rhodamine B and fluorescein as high-pressure indicators. The red shift of the emission peak maxima of rhodamine B and fluorescein are investigated in dependence of pressure up to 200 MPa using as the solvents water, ethanol and poly(dimethylsiloxane (PDMS with rhodamine B and water, polystyrene beads and melamine resin beads with fluorescein. Emission spectra recording and peak fitting is done automatically at time intervals of down to a second and with 0.3 nm wavelength resolution. The wavenumber-pressure relation for rhodamine B reveals increasing divergence from linear behavior in the sequence of the solvents water, ethanol and silicone rubber. Graphical correlation of the data diverging only slightly from linearity with a selection of polarity functions is enabled using the concept of ‘deviation from linearity (DL’ plots. Using the example of rhodamine B dissolved in PDMS elastomer it is shown that there is a temperature induced irreversible molecular reordering, when scanning between 3 and ∼50°C, and a polarity change in the proximity of the embedded dye molecule. Swelling studies are performed with PDMS containing rhodamine B, where the elastomer is first put in water, then in ethanol and again in water. There a complex solvent exchange process is revealed in the elastomer demonstrating the feasibility of fluorescence spectroscopy, when observing variations in wavelength, to indicate and enlighten molecular rearrangements and swelling dynamics in the polymer, and polarity changes and solvent exchange processes in the dye solvation shell.

  11. Efficient Cycloaddition Reaction of Carbon Dioxide with Epoxide by Rhodamine Based Catalyst Under 1 atm Pressure

    Energy Technology Data Exchange (ETDEWEB)

    Gong, Qing; Luo, Huadong; Cao, Di; Zhang, Haibo; Wang, Wenjing; Zhou, Xiaohai [Wuhan University, Wuhan (China)

    2012-06-15

    Rhodamine B (RhB) and rhodamine 6G (Rh6G) were employed as catalysts for the synthesis of cyclic carbonate from carbon dioxide and epoxide. It turned out that the catalytic activity of Rh6G was nearly 29 times higher than that of RhB at 1 atm pressure, 90 .deg. C. Furthermore, the catalytic efficiency of RhB and Rh6G was greatly enhanced with triethylamine as co-catalyst. Under the optimized conditions, the best isolated yield (93%) of cyclic carbonate was achieved without organic solvent and metal component

  12. Staining diatoms with rhodamine dyes: control of emission colour in photonic biocomposites

    Science.gov (United States)

    Kucki, Melanie; Fuhrmann-Lieker, Thomas

    2012-01-01

    The incorporation of rhodamine dyes in the cell wall of diatoms Coscinodiscus granii and Coscinodiscus wailesii for the production of luminescent hybrid nanostructures is investigated. By systematic variation of the substitution pattern of the rhodamine core, we found that carbonic acids are considerably better suited than esters because of their physiological compatibility. The amino substitution pattern that controls the optical properties of the chromophore has no critical influence on dye uptake and incorporation, thus a variety of biocomposites with different emission maxima can be prepared. Applications in biomineralization studies as well as in materials science are envisioned. PMID:21865248

  13. A Dansyl-Rhodamine Based Fluorescent Probe for Detection of Hg2+ and Cu2.

    Science.gov (United States)

    Yuan, Shizhuang; Su, Wei; Wang, Enju

    2017-09-01

    A novel fluorescent probe based on dansyl-appended rhodamine B was developed. The probe can selectively recognize and sense Hg2+ and Cu2+ from other common metal ions by showing unique fluorescence and absorption characteristics. In MeCN/HEPES buffer solution, the probe gives a ratiometric fluorescent response to Hg2+, which was ascribed to the fluorescence resonance energy transfer from dansyl moiety to the ring-opened rhodamine B moiety, while the presence of Cu2+ causes fluorescence quenching. Beside the fluorescence change, the presence of Cu2+ and Hg2+ can induce intensive absorption at about 555 nm, which resulted in a color change from colorless to pink.

  14. Near-Infrared Phosphorus-Substituted Rhodamine with Emission Wavelength above 700 nm for Bioimaging.

    Science.gov (United States)

    Chai, Xiaoyun; Cui, Xiaoyan; Wang, Baogang; Yang, Fan; Cai, Yi; Wu, Qiuye; Wang, Ting

    2015-11-16

    Phosphorus has been successfully fused into a classic rhodamine framework, in which it replaces the bridging oxygen atom to give a series of phosphorus-substituted rhodamines (PRs). Because of the electron-accepting properties of the phosphorus moiety, which is due to effective σ*-π* interactions and strengthened by the inductivity of phosphine oxide, PR exhibits extraordinary long-wavelength fluorescence emission, elongating to the region above 700 nm, with bathochromic shifts of 140 and 40 nm relative to rhodamine and silicon-substituted rhodamine, respectively. Other advantageous properties of the rhodamine family, including high molar extinction coefficient, considerable quantum efficiency, high water solubility, pH-independent emission, great tolerance to photobleaching, and low cytotoxicity, stay intact in PR. Given these excellent properties, PR is desirable for NIR-fluorescence imaging in vivo. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Molecular dynamics simulation of the effect of pH on the adsorption of rhodamine laser dyes on TiO2 hydroxylated surfaces

    OpenAIRE

    2009-01-01

    Abstract We have carried out a study of the adsorption, on the (101) surface of anatase TiO2, of two industrially relevant rhodamines molecules (rhodamine 6G and rhodamine 800) employing Molecular Dynamics. These theoretical studies have shown that Rhodamine 6G must adsorb on surfaces under basic conditions. Moreover, the adsorption of this molecule shows a strong dependence upon the pH of the system, i.e. under neutral conditions the adsorption energy is quite smaller, and under ...

  16. Low concentrations of Rhodamine-6G selectively destroy tumor cells and improve survival of melanoma transplanted mice.

    Science.gov (United States)

    Kutushov, M; Gorelik, O

    2013-01-01

    Rhodamine-6G is a fluorescent dye binding to mitochondria, thus reducing the intact mitochondria number and inhibiting mitochondrial metabolic activity. Resultantly, the respiratory chain functioning becomes blocked, the cell "suffocated" and eventually destroyed. Unlike normal cells, malignant cells demonstrate a priori reduced mitochondrial numbers and aberrant metabolism. Therefore, a turning point might exist, when Rhodamine-induced loss of active mitochondria would selectively destroy malignant, but spare normal cells. Various malignant vs. non-malignant cell lines were cultured with Rhodamine-6G at different concentrations. In addition, C57Bl mice were implanted with B16-F10 melanoma and treated with Rhodamine-6G at different dosage/time regimens. Viability and proliferation of cultured tumor cells were time and dose-dependently inhibited, up to 90%, by Rhodamine-6G, with profound histological signs of cell death. By contrast, inhibition of normal control cell proliferation hardly exceeded 15-17%. Melanoma-transplanted mice receiving Rhodamine-6G demonstrated prolonged survival, improved clinical parameters, inhibited tumor growth and metastases count, compared to their untreated counterparts. Twice-a-week 10-6M Rhodamine-6G regimen yielded the most prominent results. We conclude that malignant, but not normal, cells are selectively destroyed by low doses of Rhodamine-6G. In vivo, such treatment selectively suppresses tumor progression and dissemination, thus improving prognosis. We suggest that selective anti-tumor properties of Rhodamine-6G are based on unique physiologic differences in energy metabolism between malignant and normal cells. If found clinically relevant, low concentrations of Rhodamine-6G might be useful for replacing, or backing up, more aggressive nonselective chemotherapeutic compounds.

  17. Rhodamine-Injected Eggs to Photographically Identify Small Nest-Predators

    Science.gov (United States)

    Thomas J. Maier; Richard M. DeGraaf

    2000-01-01

    Photographs that clearly disclose avian-nest predators are difficult to obtain, particularly when predators are small and exhibit subtle depredatory behavior. We exposed House Sparrow (Passer domesticus) eggs injected with Rhodamine B dye in camera-monitored ground nests for 12-d periods at 76 sites within mixed-hardwood forest stands in central...

  18. Influence of Ti–O–Si hetero-linkages in the photocatalytic degradation of Rhodamine B

    NARCIS (Netherlands)

    Rasalingam, S; Kibombo, H.S.; Wu, C.M.; Budhi, S.; Peng, R.; Baltrusaitis, Jonas; Koodali, R.T.

    2013-01-01

    The influence of Ti–O–Si hetero-linkages in the degradation of Rhodamine B (RhB) dye over TiO2–SiO2 xerogels is exemplified by XPS analysis. We demonstrate a relationship between the percentage surface content of Ti–O–Si and the rate of photocatalytic degradation of RhB. Our detailed surface

  19. A dansyl-rhodamine chemosensor for Fe(III) based on off-on FRET.

    Science.gov (United States)

    Piao, Jingyu; Lv, Jia; Zhou, Xin; Zhao, Tong; Wu, Xue

    2014-07-15

    A novel fluorescent chemosensor bearing a rhodamine and a dansyl moiety was developed for highly selective detection of Fe(3+) based on fluorescence resonance energy transfer (FRET) mechanism. Binding of Fe(3+) to the chemosensor induced spirolactam ring opening in the rhodamine moiety and subsequent off-on FRET from the dansyl energy donor to the rhodamine energy acceptor due to the spectral overlap between the emission of the dansyl moiety and the absorption of the ring opened rhodamine moiety. Job's plot analysis indicated a 1:1 binding stoichiometry between the chemosensor and Fe(3+). The association constant was estimated to be 2.72×10(3) M(-1) according to the Benesi-Hildebrand method. With the feature of easy synthesis, simple structural skeleton and excellent sensing ability, the newly synthesized chemosensor provided the potential for applying as a highly selective fluorescent probe in complex samples containing various competitive metal ions and developing other metal ion chemosensors to fulfill various needs of biological and environmental field. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Highly luminescent hybrid materials based on smectites with polyethylene glycol modified with rhodamine fluorophore

    Czech Academy of Sciences Publication Activity Database

    Sas, S.; Danko, M.; Bizovská, V.; Lang, Kamil; Bujdák, J.

    2017-01-01

    Roč. 138, MAR (2017), s. 25-33 ISSN 0169-1317 Institutional support: RVO:61388980 Keywords : Luminescent polymers * Rhodamine B * Molecular aggregation * Fluorescence * Optical materials Subject RIV: CA - Inorganic Chemistry OBOR OECD: Inorganic and nuclear chemistry Impact factor: 3.101, year: 2016

  1. A dansyl-rhodamine chemosensor for Fe(III) based on off-on FRET

    Science.gov (United States)

    Piao, Jingyu; Lv, Jia; Zhou, Xin; Zhao, Tong; Wu, Xue

    2014-07-01

    A novel fluorescent chemosensor bearing a rhodamine and a dansyl moiety was developed for highly selective detection of Fe3+ based on fluorescence resonance energy transfer (FRET) mechanism. Binding of Fe3+ to the chemosensor induced spirolactam ring opening in the rhodamine moiety and subsequent off-on FRET from the dansyl energy donor to the rhodamine energy acceptor due to the spectral overlap between the emission of the dansyl moiety and the absorption of the ring opened rhodamine moiety. Job's plot analysis indicated a 1:1 binding stoichiometry between the chemosensor and Fe3+. The association constant was estimated to be 2.72 × 103 M-1 according to the Benesi-Hildebrand method. With the feature of easy synthesis, simple structural skeleton and excellent sensing ability, the newly synthesized chemosensor provided the potential for applying as a highly selective fluorescent probe in complex samples containing various competitive metal ions and developing other metal ion chemosensors to fulfill various needs of biological and environmental field.

  2. KELAINAN PATOLOGI PADA MENCIT DAN TIKUS DISEBABKAN ZAT WARNA RHODAMINE B DAN METANIL YELLOW

    Directory of Open Access Journals (Sweden)

    Iwan T. Budiarso

    2012-09-01

    Full Text Available PATHOLOGICAL LESIONS IN MICE AND RATS CAUSED BY DYESTUFFS RHODAMINE B AND METANIL YELLOW. Rhodamine B and Metanil Yellow are 2 non-edible dyestuffs which are widely used for coloring snacks and drinks in Jakarta. These substances are reportedly toxic for human beings, however no data on acute or chronic intoxications are available so far. Groups of mice and rats were fed with either Rhodamine B or Metanil Yellow. The doses of these dyestuffs varied from 0.5 mg to 1350 mg per kilogram body weight. These animals were dcvided into 3 different experimental groups, respectively acute, subacute and chronic toxicity tests. Clinical signs included discoloration of the skin and its intensity depended upon the concentration of the dyestuffs used. The body weight gain of the test animals were consistently lower than those of the controls. Some animals became agressive and cannibalism occurred. Pathological lesions consisted of unthriftiness, focal liver inflammation, hydronephrosis, hepatoma and lymphoma. Considering the results of the experiments, it is justified to warn that the wide use of Rhodamine B and Metanil Yellow for food coloring might be hazardous for human health.

  3. DETEKSI KANDUNGAN RHODAMIN B PADA SAUS SERTA CEMARAN BORAKS DAN BAKTERI SALMONELLA SP. PADACILOK KELILING SALATIGA

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    Ardhikajaya Wahyu Prasetya

    2017-01-01

    Full Text Available ABSTRACT Borax and rhodamine B are preservative and dye banned to be used in food products. However, some traders still use borax and rhodamine B to their food and drink products. Cilok is a kind of food liked by schoolchildren and categorized as street vendor food (PKL. Most of food sold by street vendor has not touched by strict control from BPOM, so its quality is not good. Pentol cilok is a food made by starch. It tastes delicious and chewy. Furthermore, it is liked by schoolchildren. Cilok is also served by adding the sauce in order to make it tastier. It is common to add dye in that sauce and the dye is not natural dye nor food coloring. In the cilok, the contamination of microbe especially Salmonella sp. is influenced by unhygienic process. This research aims to find out borax and Salmonella sp. contamination and also the existence of rhodamine B on the cilok sauce. The methods used in this research are qualitative, quantitative, and the detection of Salmonella sp. contamination. The result shows, of the 8 tested samples, 4 samples (sample B, C, E, and H contain little amount of borax, the sauce contains no rhodamine B. This can be seen from Rf value and color reaction test. On the testing of Salmonella sp., there are bacteria before boiling process (when cilok is still in the form of dough and there are no bacteria after boiling and steaming process, so it is safe to be consumed.

  4. Preparation of Rhodamine B Fluorescent Poly(methacrylic acid Coated Gelatin Nanoparticles

    Directory of Open Access Journals (Sweden)

    Zhenhai Gan

    2011-01-01

    Full Text Available Poly(methacrylic acid (PMAA-coated gelatin nanoparticles encapsulated with fluorescent dye rhodamine B were prepared by the coacervation method with the aim to retard the release of rhodamine B from the gelatin matrix. With sodium sulfate as coacervation reagent for gelatin, a kind of biopolymer with excellent biocompatibility, the formed gelatin nanoparticles were cross-linked by formaldehyde followed by the polymerization of methacrylic acid coating. The fluorescent poly(methacrylic acid coated gelatin (FPMAAG nanoparticles had a uniform spherical shape and a size distribution of 60±5 nm. Infrared spectral analysis confirmed the formation of PMAA coating on the gelatin nanoparticles. Based on UV-Vis spectra, the loading efficiency of rhodamine B for the FPMAAG nanoparticles was 0.26 μg per mg nanoparticles. The encapsulated rhodamine B could sustain for two weeks. Favorable fluorescence property and fluorescence imaging of cells confirmed that the FPMAAG nanoparticles have promising biochemical, bioanalytical, and biomedical applications.

  5. Reversible photochromic system based on rhodamine B salicylaldehyde hydrazone metal complex.

    Science.gov (United States)

    Li, Kai; Xiang, Yu; Wang, Xiaoyan; Li, Ji; Hu, Rongrong; Tong, Aijun; Tang, Ben Zhong

    2014-01-29

    Photochromic molecules are widely applied in chemistry, physics, biology, and materials science. Although a few photochromic systems have been developed before, their applications are still limited by complicated synthesis, low fatigue resistance, or incomplete light conversion. Rhodamine is a class of dyes with excellent optical properties including long-wavelength absorption, large absorption coefficient, and high photostability in its ring-open form. It is an ideal chromophore for the development of new photochromic systems. However, known photochromic rhodamine derivatives, such as amides, exhibit only millisecond lifetimes in their colored ring-open forms, making their application very limited and difficult. In this work, rhodamine B salicylaldehyde hydrazone metal complex was found to undergo intramolecular ring-open reactions upon UV irradiation, which led to a distinct color and fluorescence change both in solution and in solid matrix. The complex showed good fatigue resistance for the reversible photochromism and long lifetime for the ring-open state. Interestingly, the thermal bleaching rate was tunable by using different metal ions, temperatures, solvents, and chemical substitutions. It was proposed that UV light promoted isomerization of the rhodamine B derivative from enol-form to keto-form, which induced ring-opening of the rhodamine spirolactam in the complex to generate color. The photochromic system was successfully applied for photoprinting and UV strength measurement in the solid state. As compared to other reported photochromic molecules, the system in this study has its advantages of facile synthesis and tunable thermal bleaching rate, and also provides new insights into the development of photochromic materials based on metal complex and spirolactam-containing dyes.

  6. Environmentally Robust Rhodamine Reporters for Probe-based Cellular Detection of the Cancer-linked Oxidoreductase hNQO1.

    Science.gov (United States)

    Best, Quinn A; Johnson, Amanda E; Prasai, Bijeta; Rouillere, Alexandra; McCarley, Robin L

    2016-01-15

    We successfully synthesized a fluorescent probe capable of detecting the cancer-associated quinoneoxidoreductase isozyme-1 within human cells, based on results from an investigation of the stability of various rhodamines and seminaphthorhodamines toward the biological reductant NADH, present at ∼100-200 μM within cells. While rhodamines are generally known for their chemical stability, we observe that NADH causes significant and sometimes rapid modification of numerous rhodamine analogues, including those oftentimes used in imaging applications. Results from mechanistic studies lead us to rule out a radical-based reduction pathway, suggesting rhodamine reduction by NADH proceeds by a hydride transfer process to yield the reduced leuco form of the rhodamine and oxidized NAD(+). A relationship between the structural features of the rhodamines and their reactivity with NADH is observed. Rhodamines with increased alkylation on the N3- and N6-nitrogens, as well as the xanthene core, react the least with NADH; whereas, nonalkylated variants or analogues with electron-withdrawing substituents have the fastest rates of reaction. These outcomes allowed us to judiciously construct a seminaphthorhodamine-based, turn-on fluorescent probe that is capable of selectively detecting the cancer-associated, NADH-dependent enzyme quinoneoxidoreductase isozyme-1 in human cancer cells, without the issue of NADH-induced deactivation of the seminaphthorhodamine reporter.

  7. PENGETAHUAN DAN PERILAKU PEDAGANG CABE MERAH GILING DALAM PENGGUNAAN RHODAMINE B DI PASAR TRADISIONAL DI DKI JAKARTA

    Directory of Open Access Journals (Sweden)

    Djarismawati Djarismawati

    2012-11-01

    Full Text Available Rhodamine B, a basic dye, is prohibited to be used in foods as it is hazardous to health. Recently, it is being used to make ground red chili and oher foods more colorful and brighter. A study was carried out in DKI Jakarta to elicit information about the knowledge and practice of ground red chili vendors using Rhodamine B in three traditional markets of Jakarta. A total of 90 samples were collected from Kramat Jati, Pasar Minggu, and Tanah Abang markets. The laboratory examination result confirmed that 67% of samples contained Rhodamine B. There were 60% of respondents who knew about the health hazard of Rhodamine B and 45% of them use if to make the ground chili a brighter red. The study also showed that there was a statistical correlation between the vendors knowledge and the use of Rhodamine B. The study suggests that regular inspection and education should be conducted to improve the knowledge of ground red chili vendors, as to increase awareness of the health hazards of Rhodamine B in foods.   Keywords: knowledge, practice, red chili, Rhodamine B

  8. Effects of single and double bonds in linkers on colorimetric and fluorescent sensing properties of polyving akohol grafting rhodamine hydrazides.

    Science.gov (United States)

    Geng, Tong-Mou; Wang, Xie; Wang, Zhu-Qing; Chen, Tai-Jie; Zhu, Hai; Wang, Yu

    2015-03-01

    Two rhodamine derivatives, N-mono-maleic acid amide-N'-rhodamine B hydrazide (MRBH) and N-mono-succinic acid amide-N'-rhodamine 6G hydrazide (SR6GH), were synthesized by amidation with maleic anhydride (MAH), succinic anhydride (SAH) and rhodamine B hydrazide, rhodamine 6G hydrazide, which were identified by FTIR, (1)H NMR and elemental analysis. Two water-soluble fluorescent materials (PVA-MRBH and PVA-SR6GH) were prepared via esterification reaction with N-mono-maleic acyl chloride amide-N'-rhodamine B hydrazide (MRBHCl) or N-mono-maleic acyl chloride amide-N'-rhodamine 6G hydrazide (SR6GHCl) and poly(vinyl alcohol) (PVA) in DMSO solution. The sensing behaviors of PVA-MRBH and PVA-SR6GH were explored by recording the fluorescence spectra in completely aqueous solution. Upon the addition of Cu(2+) and Fe(3+) ions to the aqueous solution of PVA-MRBH, visual color change from rose pink to amaranth and orange for Cu(2+) and Fe(3+) ions, respectively, and fluorescence quenching were observed. Titration of Cu(2+), Fe(3+), Cr(3+) or Hg(2+) into the aqueous solution of PVA-SR6GH, although they induced fluorescence enhancement, only Fe(3+) made the color changing from colorless to yellow. Moreover, other metal ions did not induce obvious changes to color and the fluorescence spectra.

  9. Selective Activation of N,N'-Diacyl Rhodamine Pro-fluorophores Paired with Releasing Enzyme, Porcine Liver Esterase (PLE).

    Science.gov (United States)

    Abney, Kristopher K; Ramos-Hunter, Susan J; Romaine, Ian M; Godwin, J Shawn; Sulikowski, Gary A; Weaver, Charles David

    2018-04-21

    This study reports the synthesis and testing of a family of rhodamine pro-fluorophores and an enzyme capable of converting pro-fluorophores to Rhodamine 110. We prepared a library of simple N,N'-diacyl rhodamines and investigated Porcine Liver Esterase (PLE) as an enzyme to activate rhodamine-based pro-fluorophores. A PLE-expressing cell line generated an increase in fluorescence rapidly upon pro-fluorophore addition demonstrating the rhodamine pro-fluorophores are readily taken up and fluorescent upon PLE-mediated release. Rhodamine pro-fluorophore amides trifluoroacetamide (TFAm) and proponamide (PAm) appeared to be the best substrates using a cell-based assay using PLE expressing HEK293. Our pro-fluorophore series showed diffusion into live cells and resisted endogenous hydrolysis. The use of our engineered cell line containing the exogenous enzyme PLE demonstrated the rigorousness of amide masking when compared to cells not containing PLE. This simple and selective pro-fluorophore rhodamine pair with PLE offers the potential to be used in vitro and in vivo fluorescence based assays. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Long-lived and largely red-shifted photoluminescence of solid-state rhodamine dyes: Molecular exciton coupling and structural effect

    International Nuclear Information System (INIS)

    Zhang, Xian-Fu; Zhang, Ya-Kui

    2015-01-01

    The optical absorption and fluorescence properties of five rhodamine dyes in solid-state are measured and show large difference from that in their gas phase or liquid solvents. All solid-state rhodamine dyes strongly absorb all light in UV and visible region, but emit only red and NIR fluorescence (680–800 nm, >100 nm red-shifted from that in solution). Further more, the absorption maxima of a solid-state rhodamine show a large red-shifted band (~100 nm) and blue-shifted peak (~125 nm) compared to that in solutions, indicating a strong molecular exciton coupling between molecules. All solid-state rhodamines still show reasonably good fluorescence quantum yield (Φ f ). In particular, solid-state Rhodamine B butyl ester and sulfonyl Rhodamine B showed a much longer emission lifetime (τ f ) than that of the corresponding molecular rhodamine, i.e. 4.12 and 4.14 ns in solid state compared to 1.61 and 2.47 ns in solution. The chemical structure of a rhodamine molecule showed dramatic effect on Φ f and τ f values for solid state rhodamine. The larger substituent in the benzene moiety favors higher Φ f and τ f values of rhodamine solids. These effects can be elucidated by the relation between structure-molecular distance and molecular exciton couplings. - Highlights: • Optical properties of solid rhodamines show large difference from that in solutions. • Solid-state rhodamine dyes emit red and NIR fluorescence (680–800 nm). • Solid-state rhodamines still show reasonably good fluorescence quantum yield. • Solid-state rhodamines have much longer fluorescence lifetimes than that in solutions

  11. Long-lived and largely red-shifted photoluminescence of solid-state rhodamine dyes: Molecular exciton coupling and structural effect

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xian-Fu, E-mail: zhangxianfu@tsinghua.org.cn [Institute of Applied Photochemistry & Center of Analysis and Measurements, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei Province (China); MPC Technologies, Hamilton, ON, Canada L8S 3H4 (Canada); Zhang, Ya-Kui [Institute of Applied Photochemistry & Center of Analysis and Measurements, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei Province (China)

    2015-10-15

    The optical absorption and fluorescence properties of five rhodamine dyes in solid-state are measured and show large difference from that in their gas phase or liquid solvents. All solid-state rhodamine dyes strongly absorb all light in UV and visible region, but emit only red and NIR fluorescence (680–800 nm, >100 nm red-shifted from that in solution). Further more, the absorption maxima of a solid-state rhodamine show a large red-shifted band (~100 nm) and blue-shifted peak (~125 nm) compared to that in solutions, indicating a strong molecular exciton coupling between molecules. All solid-state rhodamines still show reasonably good fluorescence quantum yield (Φ{sub f}). In particular, solid-state Rhodamine B butyl ester and sulfonyl Rhodamine B showed a much longer emission lifetime (τ{sub f}) than that of the corresponding molecular rhodamine, i.e. 4.12 and 4.14 ns in solid state compared to 1.61 and 2.47 ns in solution. The chemical structure of a rhodamine molecule showed dramatic effect on Φ{sub f} and τ{sub f} values for solid state rhodamine. The larger substituent in the benzene moiety favors higher Φ{sub f} and τ{sub f} values of rhodamine solids. These effects can be elucidated by the relation between structure-molecular distance and molecular exciton couplings. - Highlights: • Optical properties of solid rhodamines show large difference from that in solutions. • Solid-state rhodamine dyes emit red and NIR fluorescence (680–800 nm). • Solid-state rhodamines still show reasonably good fluorescence quantum yield. • Solid-state rhodamines have much longer fluorescence lifetimes than that in solutions.

  12. Broadband Light-Harvesting Molecular Triads with High FRET Efficiency Based on the Coumarin-Rhodamine-BODIPY Platform.

    Science.gov (United States)

    He, Longwei; Zhu, Sasa; Liu, Yong; Xie, Yinan; Xu, Qiuyan; Wei, Haipeng; Lin, Weiying

    2015-08-17

    Broadband capturing and FRET-based light-harvesting molecular triads, CRBs, based on the coumarin-rhodamine-BODIPY platform were rationally designed and synthesized. The absorption band of CRBs starts from blue-green to yellow-orange regions (330-610 nm), covering the strong radiation scope of sunlight. The peripheral coumarin and BODIPY chromophore energy could transfer to the central acceptor rhodamine by a one-step direct way. The energy of the coumarin moiety could also transfer to the BODIPY unit, subsequently transferring to the rhodamine core by two-step sequential ways. Both the efficiencies of the coumarin moiety and the BODIPY unit to the rhodamine core in CRBs, determined by two different ways, are very high. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Chemiluminescence behavior based on oxidation reaction of rhodamine B with cerium(IV) in sulfuric acid medium

    International Nuclear Information System (INIS)

    Ma Yongjun; Jin Xiaoyong; Zhou Min; Zhang Ziyu; Teng Xiulan; Chen Hui

    2003-01-01

    The chemiluminescence (CL) of the rhodamine B (RhB)-cerium(IV) system was investigated by flow-injection. Rhodamine B was suggested to be a suitable chemiluminescent reagent in acidic conditions. When the concentration of rhodamine B was 100 mg l -1 and cerium sulfate was 1.6 mmol l -1 in sulfuric acid, the chemiluminescent intensity was found to be highest by using 0.3 mol l -1 sulfuric acid as a carrier solution. The particular chemiluminescent system could tolerate such distinct acidic environments that it was utilized for detecting many compounds that are stable in acidic solutions. Furthermore, by virtue of IR, UV-Vis and luminescence spectroscopic measurements, the chemiluminescent behavior of rhodamine B was studied and a possible mechanism for this chemiluminescent reaction was proposed. The emitter was affirmed to be a radical species due to one of the oxidation products of RhB; the chemiluminescent emissive wavelength was about 425 nm

  14. Turn-On Fluorescent Chemosensor for Hg2+ Based on Multivalent Rhodamine Ligands

    Science.gov (United States)

    Wang, Xuemei; Iqbal, Mudassir; Huskens, Jurriaan; Verboom, Willem

    2012-01-01

    Rhodamine-based fluorescent chemosensors 1 and 2 exhibit selective fluorescence enhancement to Fe3+ and Hg2+ over other metal ions at 580 nm in CH3CN/H2O (3/1, v/v) solution. Bis(rhodamine) chemosensor 1, under optimized conditions (CH3CN/HEPES buffer (0.02 M, pH = 7.0) (95/5, v/v)), shows a high selectivity and sensitivity to Hg2+, with a linear working range of 0–50 μM, a wide pH span of 4–10, and a detection limit of 0.4 μM Hg2+. PMID:23222686

  15. Characteristics of a Broadband Dye Laser Using Pyrromethene and Rhodamine Dyes

    Science.gov (United States)

    Tedder, Sarah A.; Danehy, Paul M.; Wheeler, Jeffrey L.

    2011-01-01

    A broadband dye laser pumped by a frequency-doubled Nd:YAG laser with a full-width half-maximum (FWHM) from 592 to 610 nm was created for the use in a dual-pump broadband CARS system called WIDECARS. The desired broadband dye laser was generated with a mixture of Pyrromethene dyes as an oscillator gain medium and a spectral selective optic in the oscillator cavity. A mixture of Rhodamine dyes were used in the amplifier dye cell. To create this laser a study was performed to characterize the spectral behavior of broadband dye lasers created with Rhodamine dyes 590, 610, and 640, Pyrromethene dyes 597 and 650 as well as mixture of these dyes.

  16. Adsorption properties of cationic rhodamine B dye onto metals chloride-activated castor bean residue carbons.

    Science.gov (United States)

    Zhi, Lee Lin; Zaini, Muhammad Abbas Ahmad

    2017-02-01

    This work was aimed to evaluate the feasibility of castor bean residue based activated carbons prepared through metals chloride activation. The activated carbons were characterized for textural properties and surface chemistry, and the adsorption data of rhodamine B were established to investigate the removal performance. Zinc chloride-activated carbon with specific surface area of 395 m 2 /g displayed a higher adsorption capacity of 175 mg/g. Magnesium chloride and iron(III) chloride are less toxic and promising agents for composite chemical activation. The adsorption data obeyed Langmuir isotherm and pseudo-second-order kinetics model. The rate-limiting step in the adsorption of rhodamine B is film diffusion. The positive values of enthalpy and entropy indicate that the adsorption is endothermic and spontaneous at high temperature.

  17. Teaching Old Dyes New Tricks: Biological Probes Built from Fluoresceins and Rhodamines.

    Science.gov (United States)

    Lavis, Luke D

    2017-06-20

    Small-molecule fluorophores, such as fluorescein and rhodamine derivatives, are critical tools in modern biochemical and biological research. The field of chemical dyes is old; colored molecules were first discovered in the 1800s, and the fluorescein and rhodamine scaffolds have been known for over a century. Nevertheless, there has been a renaissance in using these dyes to create tools for biochemistry and biology. The application of modern chemistry, biochemistry, molecular genetics, and optical physics to these old structures enables and drives the development of novel, sophisticated fluorescent dyes. This critical review focuses on an important example of chemical biology-the melding of old and new chemical knowledge-leading to useful molecules for advanced biochemical and biological experiments.

  18. Spectrophotometric procedure using rhodamine B for determination of submicrogram quantities of antimony in rocks

    Science.gov (United States)

    Schnepfe, M.M.

    1973-01-01

    A spectrophotometric procedure using Rhodamine B is given for the determination of antimony in mineralized rocks after its separation as stibine. A study of the Rhodamine B reaction points to the importance of the order of addition of reagents in enhancing sensitivity and increasing the stability of the system. The tolerance of some 26 elements is established for the overall procedure. Although the limit of determination is approximately 0??5 ppm Sb in a 0??2-g sample, the procedure is intended primarily for screening samples containing more than 1 ppm Sb. In pure solutions 0??1 ??g of antimony can be determined with a relative standard deviation of 25%. For >0??2 ??g of antimony a relative standard deviation of 15% or less can be expected. ?? 1973.

  19. Ratiometric Signaling of Hypochlorite by the Oxidative Cleavage of Sulfonhydrazide-Based Rhodamine-Dansyl Dyad.

    Science.gov (United States)

    Lee, Hyo Jin; Cho, Min Jeoung; Chang, Suk-Kyu

    2015-09-08

    A reaction-based probe 1 for hypochlorite signaling was designed by the conjugation of two fluorophores, rhodamine and dansyl moieties, by the reaction of rhodamine B base with dansylhydrazine. Probe 1 exhibited pronounced hypochlorite-selective chromogenic and fluorescent signaling behavior over other oxidants used in practical applications, such as hydrogen peroxide, peracetic acid, and ammonium persulfate, as well as commonly encountered metal ions and anions. Signaling was attributed to the hypochlorite-induced oxidative cleavage of the sulfonhydrazide linkage of the probe. In particular, favorable ratiometric fluorescence signaling was possible by utilizing the emissions of the two fluorophores. A detection limit of 1.13 × 10(-6) M (0.058 ppm) was estimated for the determination of hypochlorite. A paper-based test strip was prepared and was used as a semiquantitative indicator for the presence of hypochlorite in aqueous solutions. The probe was also successfully applied for the determination of hypochlorite in practical tap water samples.

  20. A thiourea-appended rhodamine chemodosimeter for mercury(II) and its bioimaging application

    Science.gov (United States)

    Tantipanjaporn, Ajcharapan; Prabpai, Samran; Suksen, Kanoknetr; Kongsaeree, Palangpon

    2018-03-01

    A rhodamine-thiourea conjugate RTP with an o-phenylenediamine linker was developed as a fluorogenic chemodosimeter for Hg2+ detection. In the presence of Hg2+, a colorless solution of RTP turned pink with a maximum absorption band at 555 nm and with a 62-fold fluorescence enhancement at 578 nm (Φ = 0.34). RTP is highly selective to Hg2+ among other metal ions with a detection limit of 1.6 nM (0.3 ppb). A similar rhodamine analog with a flexible ethylenediamine spacer was less selective and less sensitive than RTP. Hg2+ induced cyclic guanylation to yield a benzimidazole moiety and a subsequent ring-opening of the spirolactam unit resulted in chromogenic and fluorogenic changes. The membrane-permeable RTP probe was successfully demonstrated in monitoring of Hg2+ in cultured HeLa cells.

  1. Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer.

    Science.gov (United States)

    Koide, Yuichiro; Urano, Yasuteru; Hanaoka, Kenjiro; Terai, Takuya; Nagano, Tetsuo

    2011-06-17

    The absorption and emission wavelengths of group 14 pyronines and rhodamines, which contain silicon, germanium, or tin at the 10 position of the xanthene chromophore, showed large bathochromic shifts compared to the original rhodamines, owing to stabilization of the LUMO energy levels by σ*-π* conjugation between group 14 atom-C (methyl) σ* orbitals and a π* orbital of the fluorophore. These group 14 pyronines and rhodamines retain the advantages of the original rhodamines, including high quantum efficiency in aqueous media (Φ(fl) = 0.3-0.45), tolerance to photobleaching, and high water solubility. Group 14 rhodamines have higher values of reduction potential than other NIR light-emitting original rhodamines, and therefore, we speculated their NIR fluorescence could be controlled through the photoinduced electron transfer (PeT) mechanism. Indeed, we found that the fluorescence quantum yield (Φ(fl)) of Si-rhodamine (SiR) and Ge-rhodamine (GeR) could be made nearly equal to zero, and the threshold level for fluorescence on/off switching lies at around 1.3-1.5 V for the SiRs. This is about 0.1 V lower than in the case of TokyoGreens, in which the fluorophore is well established to be effective for PeT-based probes. That is to say, the fluorescence of SiR and GeR can be drastically activated by more than 100-fold through a PeT strategy. To confirm the validity of this strategy for developing NIR fluorescence probes, we employed this approach to design two kinds of novel fluorescence probes emitting in the far-red to NIR region, i.e., a series of pH-sensors for use in acidic environments and a Zn(2+) sensor. We synthesized these probes and confirmed that they work well.

  2. Ion associates of rare earth elements with salicylic acid derivatives and rhodamine B and their analytical application

    Energy Technology Data Exchange (ETDEWEB)

    Tselik, E I; Poluehktov, N S; Mishchenko, V T [AN Ukrainskoj SSR, Odessa. Fiziko-Khimicheskij Inst.

    1979-10-01

    The determination of rare earth elements by extraction photometry (fluorimetric) technique with the use of salicylic acid derivatives and Rhodamine B is reported. The best results in the determination of REE in the form of ionic associates between their acidocomplexes and Rhodamine B are obtained with the use of 3,5-diiodinesalicylic acid. The ratio between components in the compounds formed and the conditions of extraction are determined.

  3. IV and IP administration of rhodamine in visualization of WBC-BBB interactions in cerebral vessels.

    Science.gov (United States)

    Reichenbach, Zachary Wilmer; Li, Hongbo; Gaughan, John P; Elliott, Melanie; Tuma, Ronald

    2015-10-01

    Epi-illuminescence intravital fluorescence microscopy has been employed to study leukocyte-endothelial interactions in a number of brain pathologies. Historically, dyes such as Rhodamine 6G have been injected intravenously. However, intravenous injections can predispose experimental animals to a multitude of complications and requires a high degree of technical skill. Here, we study the efficacy of injecting Rhodamine 6G into the peritoneum (IP) for the purpose of analyzing leukocyte-endothelial interactions through a cranial window during real time intravital microscopy. After examining the number of rolling and adherent leukocytes through a cranial window, we found no advantage to the intravenous injection (IV). Additionally, we tested blood from both routes of injection by flow cytometry to gain a very precise picture of the two methods. The two routes of administration failed to show any difference in the ability to detect cells. The study supports the notion that IP Rhodamine 6G works as efficaciously as IV and should be considered a viable alternative in experimental design for investigations employing intravital microscopy. Facilitated intravital studies will allow for more exploration into cerebral pathologies and allow for more rapid translation from the laboratory to the patient with less chance of experimental error from failed IV access. © 2015 Wiley Periodicals, Inc.

  4. A selectively rhodamine-based colorimetric probe for detecting copper(II) ion.

    Science.gov (United States)

    Zhang, Jiangang; Zhang, Li; Wei, Yanli; Chao, Jianbing; Shuang, Shaomin; Cai, Zongwei; Dong, Chuan

    2014-11-11

    A novel rhodamine derivative 3-bromo-5-methylsalicylaldehyde rhodamine B hydrazone (BMSRH) has been synthesized by reacting rhodamine B hydrazide with 3-bromo-5-methylsalicylaldehyde and developed as a new colorimetric probe for the selective and sensitive detection of Cu2+. Addition of Cu2+ to the solution of BMSRH results in a rapid color change from colorless to red together with an obvious new band appeared at 552 nm in the UV-vis absorption spectra. This change is attributed to the spirocycle form of BMSRH opened via coordination with Cu2+ in a 1:1 stoichiometry and their association constant is determined as 3.2×10(4) L mol(-1). Experimental results indicate that the BMSRH can provide a rapid, selective and sensitive response to Cu2+ with a linear dynamic range 0.667-240 μmol/L. Common interferent ions do not show any interference on the Cu2+ determination. It is anticipated that BMSRH can be a good candidate probe and has potential application for Cu2+ determination. The proposed probe exhibits the following advantages: a quick, simple and facile synthesis. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Multi step FRET among three laser dyes Pyrene, Acriflavine and Rhodamine B

    International Nuclear Information System (INIS)

    Saha, Jaba; Dey, Dibyendu; Roy, Arpan Datta; Bhattacharjee, D.; Hussain, Syed Arshad

    2016-01-01

    Fluorescence Resonance Energy Transfer (FRET) system using three dyes has been demonstrated. It has been observed that multi step energy transfer occurred from Pyrene to Rhodamine B via Acriflavine. Here Acriflavine acts as an antenna to receive energy from Pyrene and transfer the same to Rhodamine B. This multi step FRET system is advantageous compared to the conventional FRET as this can be used to study molecular level interaction beyond conventional FRET distance (1–10 nm) as well as studying multi-branched macromolecules. The introduction of clay enhances the FRET efficiencies among the dye pair, which is an advantage to make the multi step system more useful. Similar approach can be used for increasing FRET efficiencies by using other dyes. - Highlights: • Multi-step FRET occurred from Pyrene (Py) to Rhodamine B (RhB) via Acriflavine (Acf). • Acf acts as an antenna to receive energy from Py and to transfer energy to RhB. • Multi-step FRET can be used to study molecular level interaction beyond 1–10 nm. • Incorporation of nanoclay laponite enhances the energy transfer efficiency.

  6. New fluorescent pH sensors based on covalently linkable PET rhodamines

    Science.gov (United States)

    Aigner, Daniel; Borisov, Sergey M.; Orriach Fernández, Francisco J.; Fernández Sánchez, Jorge F.; Saf, Robert; Klimant, Ingo

    2012-01-01

    A new class of rhodamines for the application as indicator dyes in fluorescent pH sensors is presented. Their pH-sensitivity derives from photoinduced electron transfer between non-protonated amino groups and the excited chromophore which results in effective fluorescence quenching at increasing pH. The new indicator class carries a pentafluorophenyl group at the 9-position of the xanthene core where other rhodamines bear 2-carboxyphenyl substituents instead. The pentafluorophenyl group is used for covalent coupling to sensor matrices by “click” reaction with mercapto groups. Photophysical properties are similar to “classical” rhodamines carrying 2′-carboxy groups. pH sensors have been prepared with two different matrix materials, silica gel and poly(2-hydroxyethylmethacrylate). Both sensors show high luminescence brightness (absolute fluorescence quantum yield ΦF≈0.6) and high pH-sensitivity at pH 5–7 which makes them suitable for monitoring biotechnological samples. To underline practical applicability, a dually lifetime referenced sensor containing Cr(III)-doped Al2O3 as reference material is presented. PMID:22967541

  7. Foam separation of Rhodamine-G and Evans Blue using a simple separatory bottle system.

    Science.gov (United States)

    Dasarathy, Dhweeja; Ito, Yoichiro

    2017-09-29

    A simple separatory glass bottle was used to improve separation effectiveness and cost efficiency while simultaneously creating a simpler system for separating biological compounds. Additionally, it was important to develop a scalable separation method so this would be applicable to both analytical and preparative separations. Compared to conventional foam separation methods, this method easily forms stable dry foam which ensures high purity of yielded fractions. A negatively charged surfactant, sodium dodecyl sulfate (SDS), was used as the ligand to carry a positively charged Rhodamine-G, leaving a negatively charged Evans Blue in the bottle. The performance of the separatory bottle was tested for separating Rhodamine-G from Evans Blue with sample sizes ranged from 1 to 12mg in preparative separations and 1-20μg in analytical separations under optimum conditions. These conditions including N 2 gas pressure, spinning speed of contents with a magnetic stirrer, concentration of the ligand, volume of the solvent, and concentration of the sample, were all modified and optimized. Based on the calculations at their peak absorbances, Rhodamine-G and Evans Blue were efficiently separated in times ranging from 1h to 3h, depending on sample volume. Optimal conditions were found to be 60psi N 2 pressure and 2mM SDS for the affinity ligand. This novel separation method will allow for rapid separation of biological compounds while simultaneously being scalable and cost effective. Published by Elsevier B.V.

  8. The influence of functional groups on the permeation and distribution of antimycobacterial rhodamine chelators.

    Science.gov (United States)

    Moniz, T; Leite, A; Silva, T; Gameiro, P; Gomes, M S; de Castro, B; Rangel, M

    2017-10-01

    We formerly hypothesized a mechanism whereby the antimycobacterial efficiency of a set of rhodamine labelled iron chelators is improved via the rhodamine fluorophore which enhances the chelators' permeation properties through membranes. To validate our hypothesis in a cellular context and to understand the influence of the structure of the fluorophore on the chelator's uptake and distribution within macrophages we now report comparative confocal microscopy studies performed with a set of rhodamine labelled chelators. We identify the functional groups of the chelator's framework that favor uptake by macrophages and conclude that the antimycobacterial effect is strongly related with the capacity of the chelator to distribute within the host cell and its compartments, a property that is closely related with the chelators' ability to interact with membranes. The quantification of the chelators' interaction with membranes was assessed through measurement of the corresponding partition constants in liposomes. The overall results support that the compounds which are preferentially taken up are the most efficient antimycobacterial chelators and for that reason we infer that the biological activity is modulated by the structural features of the fluorophore. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Toward Protein Structure In Situ: Comparison of Two Bifunctional Rhodamine Adducts of Troponin C

    Science.gov (United States)

    Julien, Olivier; Sun, Yin-Biao; Knowles, Andrea C.; Brandmeier, Birgit D.; Dale, Robert E.; Trentham, David R.; Corrie, John E. T.; Sykes, Brian D.; Irving, Malcolm

    2007-01-01

    As part of a program to develop methods for determining protein structure in situ, sTnC was labeled with a bifunctional rhodamine (BR or BSR), cross-linking residues 56 and 63 of its C-helix. NMR spectroscopy of the N-terminal domain of BSR-labeled sTnC in complex with Ca2+ and the troponin I switch peptide (residues 115–131) showed that BSR labeling does not significantly affect the secondary structure of the protein or its dynamics in solution. BR-labeling was previously shown to have no effect on the solution structure of this complex. Isometric force generation in isolated demembranated fibers from rabbit psoas muscle into which BR- or BSR-labeled sTnC had been exchanged showed reduced Ca2+-sensitivity, and this effect was larger with the BSR label. The orientation of rhodamine dipoles with respect to the fiber axis was determined by polarized fluorescence. The mean orientations of the BR and BSR dipoles were almost identical in relaxed muscle, suggesting that both probes accurately report the orientation of the C-helix to which they are attached. The BSR dipole had smaller orientational dispersion, consistent with less flexible linkers between the rhodamine dipole and cysteine-reactive groups. PMID:17483167

  10. Tests of rhodamine WT dye for toxicity to oysters and fish

    Science.gov (United States)

    Parker, Garald G.

    1973-01-01

    Because of the toxicity to oyster larvae and eggs of rhodamine B dye in concentrations greater than 1 mg/l in earlier tests, there was a concern that rhodamine WT, a similar tracer dye, would have a detrimental effect on marine life being developed under the aquaculture program of the Lummi Indian Tribe near Bellingham, Wash. Tests showed that 48-hour exposures at 24° C of 11,000 oyster eggs per liter and 6,000 12-day-old larvae per liter, in sea water with concentrations of rhodamine WT ranging from 1 μg/l to 10 mg/l, resulted in development of the eggs to normal straight-hinge larvae and no abnormalities in the larvae development. Tests made on the smolt of both silver salmon and Donaldson trout, with the fish held for 17.5 hours in a tankfull of sea water with a dye concentration of 10 mg/l at 22°C showed no mortalities or respiratory problems. With the concentration increased to 375 mg/l, and the time extended an additional 3.2 hours, still no mortalities or abnormalities were noted. The fish remained healthy in dye-free water when last checked a month after the test.

  11. Advanced oxidation of rhodamine B with hydrogen peroxide over ZnCr layered double hydroxide catalysts

    Directory of Open Access Journals (Sweden)

    Nguyen Tien Thao

    2017-09-01

    Full Text Available Zn/Cr layered zinc hydroxide materials with different molar ratios of Cr/Zn have been synthesized through the coprecipitation method at pH of 9.0–9.5. At high Cr/Zn molar ratios of 0.5/1–1/3, the materials possess some layered structure with carbonate anions between the interlayer galleries. The catalysts present uniform particle sizes and quite high surface area. An isomorphous substitution of Zn2+ by Cr3+ in the brucite-like sheets makes the layered Cr-doped zinc hydroxides potential catalysts for efficient oxidation of rhodamine B with H2O2 solution. The experimental results indicated that the intra-lattice Cr3+ ions are more active than Cr2O3 components in the oxidative removal of rhodamine B. The degradation efficiency is dependent on the intra lattice Cr3+ contents and reaction variables. The Cr/Zn LDH gave a high decolorization (99% of rhodamine B at near neutral pH and room temperature.

  12. [Fluorescence Determination of Trace Se with the Hydride-K13-Rhodamine 6G System].

    Science.gov (United States)

    Liang, Ai-hui; Li, Yuan; Huang, Shan-shan; Luo, Yang-he; Wen, Gui-qing; Jiang, Zhi-liang

    2015-05-01

    Se is a necessary trace element for human and animals, but the excess intake of Se caused poison. Thus, it is very important to determination of Se in foods and water. The target of this study is development of a new, sensitive and selective hydride generation-molecular fluorescence method for the determination of Se. In 0. 36 mol . L-1 sulfuric acid, NaBH4 as reducing agent, Se (IV) is reduced to H2 Se. Usin3-g I solution as absorption liquid3, I- is reduced to I- by H2Se. When adding rhodamine 6G, Rhodamine 6G and I3- form association particles, which lead to the fluorescence intensity decreased. When Se(IV) existing, Rhodamine 6G and I3- bind less, And the remaining amount of Rhodamine 6G increase. So the fluorescence intensity is enhanced. The analytical conditions were optimized, a 0. 36 ml . L-1 H2SO4, 21. 6.g . L-1 NaBH4, 23.3 µm . L-1 rhodamine 6G, and 50 µmol . L-1 KI3 were chosen for use. When the excitation wavelength is at 480nm, the Rayleigh scattering peak does not affect the fluorescence recording, and was selected for determination of Se. Under the selected conditions, Se(IV) concentration in the 0. 02~0. 60 µg . mL-1 range and the increase value of the fluorescence intensity (ΔF) at 562 nm linear relationship. The linear regression equation is ΔF562 nm =12. 6c + 20. 9. The detecton limit was 0.01 µ.g . L-1. The influence of coexistence substances on the hydride generatin-molecular fluorescence determination of 5. 07 X10(-6) mol . L-1 Se(IV) was considered in details. Results showed that this new fluorescence method is of high selectivity, that is, 0. 5 mmol. L-1 Ba2+, Ca2+, Zn2+ and Fe3+, 0. 25 mmol . L-1 . Mg2+, 0. 05 mmol . L-1 K+, 0. 2 mmol . L-1 Al3+, 0. 025 mmol . L-1 Te(VI) do not interfere with the determination. The influence of Hg2+, CD2+ and Cu2+ that precipitate with Se(IV), can be eliminated by addition of complex reagent. This hydride generation-molecular fluorescence method has been applied to determination of trace Se in water

  13. Biological characterization of F-18-labeled rhodamine B, a potential positron emission tomography perfusion tracer.

    Science.gov (United States)

    Bartholomä, Mark D; He, Huamei; Pacak, Christina A; Dunning, Patricia; Fahey, Frederic H; McGowan, Francis X; Cowan, Douglas B; Treves, S Ted; Packard, Alan B

    2013-11-01

    Myocardial infarction is the leading cause of death in western countries, and positron emission tomography (PET) plays an increasing role in the diagnosis and treatment planning for this disease. However, the absence of an (18)F-labeled PET myocardial perfusion tracer hampers the widespread use of PET in myocardial perfusion imaging (MPI). We recently reported a potential MPI agent based on (18)F-labeled rhodamine B. The goal of this study was to more completely define the biological properties of (18)F-labeled rhodamine B with respect to uptake and localization in an animal model of myocardial infarction and to evaluate the uptake (18)F-labeled rhodamine B by cardiomyocytes. A total of 12 female Sprague Dawley rats with a permanent ligation of the left anterior descending artery (LAD) were studied with small-animal PET. The animals were injected with 100-150 μCi of (18)F-labeled rhodamine B diethylene glycol ester ([(18)F]RhoBDEGF) and imaged two days before ligation. The animals were imaged again two to ten days post-ligation. After the post-surgery scans, the animals were euthanized and the hearts were sectioned into 1mm slices and myocardial infarct size was determined by phosphorimaging and 2,3,5-triphenyltetrazolium chloride staining (TTC). In addition, the uptake of [(18)F]RhoBDEGF in isolated rat neonatal cardiomyocytes was determined by fluorescence microscopy. Small-animal PET showed intense and uniform uptake of [(18)F]RhoBDEGF throughout the myocardium in healthy rats. After LAD ligation, well defined perfusion defects were observed in the PET images. The defect size was highly correlated with the infarct size as determined ex vivo by phosphorimaging and TTC staining. In vitro, [(18)F]RhoBDEGF was rapidly internalized into rat cardiomyocytes with ~40 % of the initial activity internalized within the 60 min incubation time. Fluorescence microscopy clearly demonstrated localization of [(18)F]RhoBDEGF in the mitochondria of rat cardiomyocytes. Fluorine-18

  14. Biological characterization of F-18-labeled rhodamine B, a potential positron emission tomography perfusion tracer

    International Nuclear Information System (INIS)

    Bartholomä, Mark D.; He, Huamei; Pacak, Christina A.; Dunning, Patricia; Fahey, Frederic H.; McGowan, Francis X.; Cowan, Douglas B.; Treves, S. Ted; Packard, Alan B.

    2013-01-01

    Introduction: Myocardial infarction is the leading cause of death in western countries, and positron emission tomography (PET) plays an increasing role in the diagnosis and treatment planning for this disease. However, the absence of an 18 F-labeled PET myocardial perfusion tracer hampers the widespread use of PET in myocardial perfusion imaging (MPI). We recently reported a potential MPI agent based on 18 F-labeled rhodamine B. The goal of this study was to more completely define the biological properties of 18 F-labeled rhodamine B with respect to uptake and localization in an animal model of myocardial infarction and to evaluate the uptake 18 F-labeled rhodamine B by cardiomyocytes. Methods: A total of 12 female Sprague Dawley rats with a permanent ligation of the left anterior descending artery (LAD) were studied with small-animal PET. The animals were injected with 100–150 μCi of 18 F-labeled rhodamine B diethylene glycol ester ([ 18 F]RhoBDEGF) and imaged two days before ligation. The animals were imaged again two to ten days post-ligation. After the post-surgery scans, the animals were euthanized and the hearts were sectioned into 1 mm slices and myocardial infarct size was determined by phosphorimaging and 2,3,5-triphenyltetrazolium chloride staining (TTC). In addition, the uptake of [ 18 F]RhoBDEGF in isolated rat neonatal cardiomyocytes was determined by fluorescence microscopy. Results: Small-animal PET showed intense and uniform uptake of [ 18 F]RhoBDEGF throughout the myocardium in healthy rats. After LAD ligation, well defined perfusion defects were observed in the PET images. The defect size was highly correlated with the infarct size as determined ex vivo by phosphorimaging and TTC staining. In vitro, [ 18 F]RhoBDEGF was rapidly internalized into rat cardiomyocytes with ∼ 40 % of the initial activity internalized within the 60 min incubation time. Fluorescence microscopy clearly demonstrated localization of [ 18 F]RhoBDEGF in the mitochondria

  15. ANALISIS ZAT PEWARNA RHODAMIN B PADA SAUS CABAI YANG BEREDAR DI KAMPUS UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

    Directory of Open Access Journals (Sweden)

    Debby Chrislia

    2017-01-01

    Full Text Available Chili sauce is one of the most popular food product because it was used as food complement. Generally in processing manufacture chili sauce added coloring substance, so product colour interesting. Nowadays more and more food manufacturers use dye that have been banned use. Rhodamine B dye in the form of a crystalline powder green or reddish purple, odorless and dissolves easily in bright red solution berfluoresan as textile dyes. Rhodamin b is still no food products were found to countain rhodamine B dye such as crackers, sauces, ice and other pastries. Rhodamin B is a synthetic dye that was banned for use in foods and is certified as a hazardous material according to Minister of Healthy of Indonesian Republic No. 722/Menkes/Per/IX/1998. Rhodamin B is carcinogenic, in the long term use can cause liver damage, kidney inflammation, and cancer. The kind of research this used a quantitative approach with the experimental methods laboratory. The purpose of this study was to determine whether there rhodamin B in the test samples using thin layer chromatography (TLC. The result shows that 7 examined samples doesn’t contain rhodamin B and and shows f count smaller than f table (F count< F table (3,61< 3,71. Synthetic dyes are contained in most of the analyzed samples are dyes that allow its use for food by the Regulation of the Minister of Health of Indonesia such as ponceau 4R and amarant and Contribution of research given by researchers of worksheets students and implementation plan learning

  16. Analisa Kandungan Rhodamin B dan Formalin pada Gula Merah Serta Pengetahuan dan Sikap Pedagang di Pasar Tradisional Kecamatan Medan Baru Tahun 2013

    OpenAIRE

    Sihombing, Emma Sari Yanti

    2014-01-01

    Rhodamine B and formaldehyde is colouration substance and preservative substance that prohibits to be utilized in food product. But there are still traders using rhodamine B and formaldehyde in food products and beverages. Brown sugar is one of the food ingredients that are often encountered in daily life. The purpose of this research is to determine the rhodamine B dye and preservative formaldehyde in brown sugar in the traditional market sub-district Medan Baru in 2013 and determine the ...

  17. Analisa Kandungan Rhodamin B dan Formalin pada Gula Merah Serta Pengetahuan dan Sikap Pedagang di Pasar Tradisional Kecamatan Medan Baru Tahun 2013

    OpenAIRE

    sihombing, emma; hasan, wirsal; marsaulina, irnawati

    2014-01-01

    Rhodamine B and formaldehyde is colouration substance and preservative substance that prohibits to be utilized in food product. But there are still traders using rhodamine B and formaldehyde in food products and beverages. Brown sugar is one of the food ingredients that are often encountered in daily life. The purpose of this research is to determine the rhodamine B dye and preservative formaldehyde in brown sugar in the traditional market sub-district Medan Baru in 2013 and determine the lev...

  18. Application of derivative and derivative ratio spectrophotometry to simultaneous trace determination of rhodamine B and rhodamine 6G after dispersive liquid-liquid microextraction.

    Science.gov (United States)

    Xiao, Ni; Deng, Jian; Huang, Kaihui; Ju, Saiqin; Hu, Canhui; Liang, Jun

    2014-07-15

    Two novel methods, first derivative spectrophotometric method ((1)D) and first derivative ratio spectrophotometric method ((1)DR), have been developed for the simultaneous trace determination of rhodamine B (RhB) and rhodamine 6G (Rh6G) in food samples after dispersive liquid-liquid microextraction (DLLME). The combination of derivative spectrophotometric techniques and DLLME procedure endows the presented methods with enhanced sensitivity and selectivity. Under optimum conditions, the linear calibration curves ranged from 5 to 450 ng mL(-1), with the correlation coefficients (r) of 0.9997 for RhB and 0.9977 for Rh6G by (1)D method, and 0.9987 for RhB and 0.9958 for Rh6G by (1)DR method, respectively. The calculated limits of detection (LODs) based on the variability of the blank solutions (S/N = 3 criterion) for 11 measurements were in the range of 0.48-1.93 ng mL(-1). The recoveries ranged from 88.1% to 111.6% (with RSD less than 4.4%) and 91.5-110.5% (with RSD less than 4.7%) for (1)D and (1)DR method, respectively. The influence of interfering substances such as foreign ions and food colorants which might be present in the food samples on the signals of RhB and Rh6G was examined. The developed methods have been successfully applied to the determination of RhB and Rh6G in black tea, red wine and chilli powder samples with the characteristics of simplicity, cost-effectiveness, environmental friendliness, and could be valuable for routine analysis. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Efflux of rhodamine from CD56+ cells as a surrogate marker for reversal of P-glycoprotein-mediated drug efflux by PSC 833

    DEFF Research Database (Denmark)

    Robey, R; Bakke, S; Stein, W

    1999-01-01

    minutes. A dose-response relationship was shown between the concentration of PSC 833 in the blood and the inhibition of rhodamine efflux, with an apparent plateau of the inhibition of rhodamine efflux at approximately 1,000 ng/mL. The Ki, defined as the concentration required for half-maximal inhibition...... of Pgp-mediated rhodamine efflux, was determined to be in the range of 29 to 181 ng/mL; although results in two patients were distinctly different, with Ki values of 914 and 916 ng/mL. MRK-16 staining was similar among all patients. We conclude that measurement of rhodamine efflux from CD56(+) cells...

  20. Derivatives of Rhodamine 19 as Mild Mitochondria-targeted Cationic Uncouplers*

    Science.gov (United States)

    Antonenko, Yuri N.; Avetisyan, Armine V.; Cherepanov, Dmitry A.; Knorre, Dmitry A.; Korshunova, Galina A.; Markova, Olga V.; Ojovan, Silvia M.; Perevoshchikova, Irina V.; Pustovidko, Antonina V.; Rokitskaya, Tatyana I.; Severina, Inna I.; Simonyan, Ruben A.; Smirnova, Ekaterina A.; Sobko, Alexander A.; Sumbatyan, Natalia V.; Severin, Fedor F.; Skulachev, Vladimir P.

    2011-01-01

    A limited decrease in mitochondrial membrane potential can be beneficial for cells, especially under some pathological conditions, suggesting that mild uncouplers (protonophores) causing such an effect are promising candidates for therapeutic uses. The great majority of protonophores are weak acids capable of permeating across membranes in their neutral and anionic forms. In the present study, protonophorous activity of a series of derivatives of cationic rhodamine 19, including dodecylrhodamine (C12R1) and its conjugate with plastoquinone (SkQR1), was revealed using a variety of assays. Derivatives of rhodamine B, lacking dissociable protons, showed no protonophorous properties. In planar bilayer lipid membranes, separating two compartments differing in pH, diffusion potential of H+ ions was generated in the presence of C12R1 and SkQR1. These compounds induced pH equilibration in liposomes loaded with the pH probe pyranine. C12R1 and SkQR1 partially stimulated respiration of rat liver mitochondria in State 4 and decreased their membrane potential. Also, C12R1 partially stimulated respiration of yeast cells but, unlike the anionic protonophore FCCP, did not suppress their growth. Loss of function of mitochondrial DNA in yeast (grande-petite transformation) is known to cause a major decrease in the mitochondrial membrane potential. We found that petite yeast cells are relatively more sensitive to the anionic uncouplers than to C12R1 compared with grande cells. Together, our data suggest that rhodamine 19-based cationic protonophores are self-limiting; their uncoupling activity is maximal at high membrane potential, but the activity decreases membrane potentials, which causes partial efflux of the uncouplers from mitochondria and, hence, prevents further membrane potential decrease. PMID:21454507

  1. Photoluminescence of urea- and urea/rhodamine B-capped TiO{sub 2} nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalo-Juan, I., E-mail: gonzalo@materials.tu-darmstadt.de; Macé, L.; Tengeler, S.; Mosallem, A.; Nicoloso, N.; Riedel, R.

    2016-07-01

    Urea- and rhodamine B (RhB)-capped TiO{sub 2} nanoparticles (NPs) have been prepared by solvothermal synthesis and characterized by HRTEM, XRD, FTIR, XPS, optical absorption and photoemission. The urea and urea/RhB ligands are capped to the surface of the TiO{sub 2} NPs for the first time through carbamate bonding. The band gap of TiO{sub 2} is slightly reduced from 3.1 eV to 3.0 eV in the urea capped TiO{sub 2} NPs (TU) and 2.9 eV for the NPs capped with urea/RhB (TUR). The generation of new trapping states in TU and TUR at the conduction band edges (surface oxygen vacancies) has been confirmed by the Urbach law providing tail state energies of 180 meV and 270 meV, respectively. These tail states are considered to be responsible for the strong reduction of the photoluminescence at ≈400 nm and the increased emission at ≈600 nm in TU and TUR. The findings suggest that urea- and RhB-capped TiO{sub 2} NPs could have potential applications as photocatalysts, opto-electronic devices, sensors, biological labels and anti-bacterial agents. - Highlights: • Urea- and urea/rhodamine B (RhB)-capped TiO{sub 2} nanoparticles preparation. • Characterization of optical properties of urea- and urea/rhodamine B (RhB)-capped TiO{sub 2} nanoparticles. • The recombination of electrons and holes is significantly reduced in the capped TiO{sub 2} nanoparticles, in comparison with TiO{sub 2}.

  2. Fluorescence spectra of Rhodamine 6G for high fluence excitation laser radiation

    CERN Document Server

    Hung, J; Olaizola, A M

    2003-01-01

    Fluorescence spectral changes of Rhodamine 6G in ethanol and glycerol solutions and deposited as a film on a silica surface have been studied using a wide range of pumping field fluence at 532 nm at room temperature. Blue shift of the fluorescence spectra and fluorescence quenching of the dye molecule in solution are observed at high excitation fluence values. Such effects are not reported for the film sample. The effects are interpreted as the result of population redistribution in the solute-solvent molecular system induced by the high fluence field and the fluence dependence of the radiationless decay mechanism.

  3. Use of Rhodamine B as a biomarker for oral plague vaccination of prairie dogs

    Science.gov (United States)

    Fernandez, Julia Rodriguez-Ramos; Rocke, Tonie E.

    2011-01-01

    Oral vaccination against Yersinia pestis could provide a feasible approach for controlling plague in prairie dogs (Cynomys spp.) for conservation and public health purposes. Biomarkers are useful in wildlife vaccination programs to demonstrate exposure to vaccine baits. Rhodamine B (RB) was tested as a potential biomarker for oral plague vaccination because it allows nonlethal sampling of animals through hair, blood, and feces. We found that RB is an appropriate marker for bait uptake studies of C. ludovicianus) when used at concentrations 10 mg RB per kg target animal mass. Whiskers with follicles provided the best sample for RB detection.

  4. Synthesis and Characterization of Rhodamine B-ethylenediamine-hyaluronan Acid as Potential Biological Functional Materials

    Science.gov (United States)

    Li, Y. L.; Wang, W. X.; Wang, Y.; Zhang, W. B.; Gong, H. M.; Liu, M. X.

    2018-05-01

    The purpose of this study is to synthesize and characterize fluorescent polymers, rhodamine B-ethylenediamine-hyaluronan acid (RhB-EA-HA). RhB-EA-HA was successfully synthesized by ester ammonolysis reaction and amidation reaction. Moreover, the structural properties of RhB-EA-HA were characterized by 1H-NMR spectra, UV-vis spectrometry and Fourier transform infrared spectroscopy (FT-IR). RhB-EA-HA can be grafted on the surface of silica nanomaterials, which may be potential biological functional materials for drug delivery system.

  5. Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells

    Science.gov (United States)

    Mitronova, Gyuzel Yu.; Sidenstein, Sven C.; Klocke, Jessica L.; Kamin, Dirk; Meineke, Dirk N. H.; D'Este, Elisa; Kraemer, Philip‐Tobias; Danzl, Johann G.

    2016-01-01

    Abstract A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm. PMID:26844929

  6. Silver nanoparticles plasmonic effect on eosin and rhodamine 6G luminescence in various media

    Science.gov (United States)

    Samusev, Ilia G.; Tikhomirova, Nadezhda S.; Slezhkin, Vasiliy A.; Zyubin, Andrey Yu.; Bryukhanov, Valery V.; Tsibulnikova, Anna V.

    2016-11-01

    The plasmonic enhancement and quenching of phosphorescence and fluorescence of the anionic (eosin) and cationic (rhodamine 6G) dyes have been studied in various environments: silver nanoparticles of silver hydrosol citrate in water, in polymer films and on the surface of nanoporous silica in order to determine the kinetic and spectral effects on the dye luminescence. Depending on the silver nanoparticles concentration both the enhancement and quenching of the dyes phosphorescence and fluorescence have been detected. The mechanism of interaction between the excited molecules and silver nanoparticles has been discussed.

  7. A turn-on fluorescent rhodamine-acyl hydrazide for selective detection of Cu{sup 2+} ions

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Jung Won; Jeong, Hyuk; Lee, Min Hee [Dept. of Chemistry, Sookmyung Women' s University, Seoul (Korea, Republic of)

    2017-03-15

    We present a rhodamine-based probe that can detect Cu{sup 2+} ions via Cu{sup 2+}-promoted hydrolysis in aqueous solutions. In solution, the probe is non-fluorescent and colorless, but gives a strong fluorescence at 586 nm and shows a color change to pink on the addition of Cu{sup 2+} ions. We demonstrate that the rhodamine-based probe undergoes Cu{sup 2+}-promoted hydrolysis with a moderate reaction time (within 3 min) and provides a fluorescence off–on change, even in the presence of other competitive metal ions. The rhodamine-based probe shows a linear correlation between increasing fluorescence at 586 nm and the Cu{sup 2+} concentration, and can detect Cu{sup 2+} at nanomolar levels (10 nM) in CH{sub 3}CN:H{sub 2}O (v/v, 90:10)

  8. Synthesis of Tb_4O_7 complexed with reduced graphene oxide for Rhodamine-B absorption

    International Nuclear Information System (INIS)

    Gao, Hui; Zhou, Yang; Chen, Keqin; Li, Xiaolong

    2016-01-01

    Highlights: • Tb–rGO composite was fabricated via a facile thermally reduction process. • The green and blue emissions were both observed in the composite. • The composite exhibited efficient absorption capability for Rhodamine-B. - Abstract: Tb_4O_7 complexed with reduced graphene oxide composite (Tb–rGO) had been designed and fabricated by a facile thermal reduction method. The formation of Tb_4O_7 particles and reduction of graphene oxide (GO) occurred simultaneously, and partial terbium ions would be complexed with rGO via oxygen-containing function groups on rGO sheets. Introducing of terbium ions could effectively tune the photoluminescence properties of rGO, and the composite exhibited the typical green emission of terbium ions as well as the blue self-luminescence of graphene entered at 440 nm. Moreover, Tb–rGO had demonstrated its high capability as an organic dye (Rhodamine-B) scavenger with high speed and efficiency. The findings showed the promising applications for large-scale removal of organic dye contaminants, especially in the field of waste water treatment.

  9. Biochemical activity of a fluorescent dye rhodamine 6G: Molecular modeling, electrochemical, spectroscopic and thermodynamic studies.

    Science.gov (United States)

    Al Masum, Abdulla; Chakraborty, Maharudra; Ghosh, Soumen; Laha, Dipranjan; Karmakar, Parimal; Islam, Md Maidul; Mukhopadhyay, Subrata

    2016-11-01

    Interaction of CT DNA with Rhodamine 6G (R6G) has been studied using molecular docking, electrochemical, spectroscopic and thermodynamic methods. From the study, it was illustrated that Rhodamine 6G binds to the minor groove of CT DNA. The binding was cooperative in nature. Circular voltametric study showed significant change in peak current and peak potential due to complexation. All the studies showed that the binding constant was in the order of 10 6 M -1 . Circular dichroic spectra showed significant conformational change on binding and DNA unwind during binding. Thermodynamic study showed that binding was favored by negative enthalpy and positive entropy change. From thermodynamic study it was also observed that several positive and negative free energies played significant role during binding and the unfavorable conformational free energy change was overcame by highly negative hydrophobic and salt dependent free energy changes. The experimental results were further validated using molecular docking study and the effect of structure on binding has been studied theoretically. From docking study it was found that the hydrophobic interaction and hydrogen bonds played a significant role during binding. The dye was absorbed by cell and this phenomenon was studied using fluorescent microscope. Cell survivability test showed that the dye active against Human Breast Cancer cells MDA-MB 468. ROS study showed that the activity is due to the production of reactive oxygen. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Laser-Induced Population Inversion in Rhodamine 6G for Lysozyme Oligomer Detection.

    Science.gov (United States)

    Hanczyc, Piotr; Sznitko, Lech

    2017-06-06

    Fluorescence spectroscopy is a common method for detecting amyloid fibrils in which organic fluorophores are used as markers that exhibit an increase in quantum yield upon binding. However, most of the dyes exhibit enhanced emission only when bound to mature fibrils, and significantly weaker signals are obtained in the presence of amyloid oligomers. In the concept of population inversion, a laser is used as an excitation source to keep the major fraction of molecules in the excited state to create the pathways for the occurrence of stimulated emission. In the case of the proteins, the conformational changes lead to the self-ordering and thus different light scattering conditions that can influence the optical signatures of the generated light. Using this methodology, we show it is possible to optically detect amyloid oligomers using commonly available staining dyes in which population inversion can be induced. The results indicate that rhodamine 6G molecules are complexed with oligomers, and using a laser-assisted methodology, weakly emissive states can be detected. Significant spectral red-shifting of rhodamine 6G dispersed with amyloid oligomers and a notable difference determined by comparison of spectra of the fibrils suggest the existence of specific dye aggregates around the oligomer binding sites. This approach can provide new insights into intermediate oligomer states that are believed to be responsible for toxic seeding in neurodegeneration diseases.

  11. Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Fluorescence Quenching of Rhodamine B.

    Science.gov (United States)

    Fael, Hanan; Sakur, Amir Al-Haj

    2015-11-01

    A novel, simple and specific spectrofluorimetric method was developed and validated for the determination of perindopril erbumine (PDE). The method is based on the fluorescence quenching of Rhodamine B upon adding perindopril erbumine. The quenched fluorescence was monitored at 578 nm after excitation at 500 nm. The optimization of the reaction conditions such as the solvent, reagent concentration, and reaction time were investigated. Under the optimum conditions, the fluorescence quenching was linear over a concentration range of 1.0-6.0 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the developed and reference methods revealed no significant differences between the methods compared in terms of accuracy and precision. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. The mechanism of rhodamine B quenching was also discussed.

  12. A simple rhodamine hydrazide-based turn-on fluorescent probe for HOCl detection.

    Science.gov (United States)

    Zhang, Zhen; Zou, Yuan; Deng, Chengquan; Meng, Liesu

    2016-06-01

    Hypochlorous acid (HOCl) plays a crucial role in daily life and mediates a variety of physiological processes, however, abnormal levels of HOCl have been associated with numerous human diseases. It is therefore of significant interest to establish a simple, selective, rapid and sensitive fluorogenic method for the detection of HOCl in environmental and biological samples. A hydrazide-containing fluorescent probe based on a rhodamine scaffold was facilely developed that could selectively detect HOCl over other biologically relevant reactive oxygen species, reactive nitrogen species and most common metal ions in vitro. Via an irreversible oxidation-hydrolysis mechanism, and upon HOCl-triggered opening of the intramolecular spirocyclic ring during detection, the rhodamine hydrazide-based probe exhibited large fluorescence enhancement in the emission spectra with a fast response, low detection limit and comparatively wide pH detection range in aqueous media. The probe was further successfully applied to monitoring trace HOCl in tap water and imaging both exogenous and endogenous HOCl within living cells. It is anticipated that this simple and useful probe might be an efficient tool with which to facilitate more HOCl-related chemical and biological research. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

  13. Rhodamine-123: a p-glycoprotein marker complex with sodium lauryl sulfate.

    Science.gov (United States)

    Al-Mohizea, Abdullah M; Al-Jenoobi, Fahad Ibrahim; Alam, Mohd Aftab

    2015-03-01

    Aim of this study was to investigate the role of sodium lauryl sulfate (SLS) as P-glycoprotein inhibitor. The everted rat gut sac model was used to study in-vitro mucosal to serosal transport of Rhodamine-123 (Rho-123). Surprisingly, SLS decreases the serosal absorption of Rho-123 at all investigated concentrations. Investigation reveals complex formation between Rhodamine-123 and sodium lauryl sulfate. Interaction profile of SLS & Rho-123 was studied at variable SLS concentrations. The SLS concentration higher than critical micelle concentration (CMC) increases the solubility of Rho-123 but could not help in serosal absorption, on the contrary the absorption of Rho-123 decreased. Rho-123 and SLS form pink color complex at sub-CMC. The SLS concentrations below CMC decrease the solubility of Rho-123. For further studies, Rho-123 & SLS complex was prepared by using solvent evaporation technique and characterized by using differential scanning calorimeter (DSC). Thermal analysis also proved the formation of complex between SLS & Rho-123. The P values were found to be significant (<0.05) except group comprising 0.0001% SLS, and that is because 0.0001% SLS is seems to be very low to affect the solubility or complexation of Rho-123.

  14. Gold nanorods-enhanced rhodamine B-permanganate chemiluminescence and its analytical application.

    Science.gov (United States)

    Hassanzadeh, Javad; Amjadi, Mohammad; Manzoori, Jamshid L; Sorouraddin, Mohammad Hossein

    2013-04-15

    A novel enhanced chemiluminescence system was developed by applying gold nanorods (Au NRs) as catalysts in rhodamine B-permanganate reaction. Au NRs with three different aspect ratios were synthesized by seed mediated growth method and characterized by UV-Vis spectra and transmission electron microscopy. It was demonstrated that Au NRs have much higher catalytic effect than spherical nanoparticles on rhodamine B-permanganate chemiluminescence reaction. Among various sizes of Au NRs, those with average aspect ratio of 3.0 were found to have the most remarkable catalytic activity. As an analytical application of the new chemiluminescence system, albumin as a model protein was quantified based on its interaction with NRs. Albumin binds to Au NRs active surfaces and inhibits their catalytic action and therefore decreases the intensity of chemiluminescence. This diminution effect is linearly related to the concentration of the human and bovine serum albumin over the ranges of 0.45-90 and 0.75-123 nmol L(-1), respectively with the corresponding limits of detection of 0.18 and 0.30 nmol L(-1). The method was successfully applied to the determination of albumin in human and bovine serum samples. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. Photocatalytic degradation of Rhodamine B dye using Fe doped TiO2 nanocomposites

    Science.gov (United States)

    Barkhade, Tejal; Banerjee, Indrani

    2018-05-01

    The unique properties of titanium dioxide (TiO2) such as high photo catalytic activity, high chemical stability and low toxicity have made it a suitable photocatalyst in recent decades. The effect of modification of TiO2 with doping of iron on its characteristics and photo catalytic efficiency was studied. The change in band gap energy of TiO2 nanoparticles after doping with Fe has been studied. Significant enhancement in photo catalytic property of TiO2 after Fe doping under light exposure conditions has been investigated. Acute exposure to non-biodegradable Rhodamine B resulted in many health problems like burning of eyes, skin irritation, nasal burning, and chest pain etc. Therefore, degradation of this dye is needed to save environment and animals. Considering the similar radius of Fe3+ and Ti4+ ions (respectively 0.64 Å and 0.68 Å), titanium position in the lattice of TiO2 can be replaced by iron cations easily. The undoped and Fe doped TiO2 nano composites were synthesized by sol-gel method, in which 1.0M% of Fe was doped with TiO2 and then the samples were characterized by using FE-SEM, UV-Visible diffuse spectroscopy, Raman Spectroscopy, and FTIR. Photo catalytic degradation of Rhodamine B dye experiment was carried out in visible light range. After 90 min time duration pink colour of dye turned colourless, indicating significant degradation rate with time.

  16. Characterization of Rhodamine Self-Assembled Films Using Desorption Electrospray Ionization Mass Spectrometry

    Science.gov (United States)

    Shi, Ruixia; Na, Na; Jiang, Fubin; Ouyang, Jin

    2013-06-01

    Growth process information and molecular structure identification are very important for characterization of self-assembled films. Here, we explore the possible application of desorption electrospray ionization mass spectrometry (DESI-MS) that provides the assembled information of rhodamine B (Rh B) and rhodamine 123 (Rh 123) films. With the help of lab-made DESI source, two characteristic ions [Rh B]+ and [Rh 123]+ are observed directly in the open environment. To evaluate the reliability of this technique, a comparative study of ultraviolet-visible (UV-vis) spectroscopy and our method is carried out, and the result shows good correlation. According to the signal intensity of characteristic ions, the layer-by-layer adsorption process of dyes can be monitored, and the thicknesses of multilayer films can also be comparatively determined. Combining the high sensitivity, selectivity, and speed of mass spectrometry, the selective adsorption of similar structure molecules under different pH is recognized easily from extracted ion chronograms. The variation trend of dyes signalling intensity with concentration of polyelectrolyte is studied as well, which reflects the effect of surface charge on dyes deposition. Additionally, the desorption area, surface morphology, and thicknesses of multilayer films are investigated using fluorescence microscope, scanning electron microscope (SEM), and atomic force microscopy (AFM), respectively. Because the desorption area was approximately as small as 2 mm2, the distribution situation of organic dyes in an arbitrary position could be gained rapidly, which means DESI-MS has advantages on in situ analysis.

  17. Off–on switchable chemosensor based on rhodamine armed with morpholine moiety

    Energy Technology Data Exchange (ETDEWEB)

    Dhara, Anamika; Guchhait, Nikhil, E-mail: nguchhait@yahoo.com; Kar, Susanta K., E-mail: skkar_cu@yahoo.co.in

    2015-12-15

    We have synthesized a novel morpholine functionalized rhodamine derivative RBM which specifically binds to Fe{sup 3+} in the presence of large excess of other competing metal ions. The chemosensor is highly selective for Fe{sup 3+} over other cations. Meanwhile, the distinct color changes and rapid switch-on fluorescence also provide “naked eyes” detection for Fe{sup 3+} over a broad pH range. The chemosensor also displays 1:1 complex formation with Fe{sup 3+} ion, with a detection limit of 2.1 µM in aqueous CH{sub 3}CN solution, showing that it may offer potential as a chemosensor for the detection of submillimolar Fe{sup 3+} ions in physiological environments. - Highlights: • Design and synthesis of morpholine functionalized rhodamine derivative RBM. • RBM is a highly selective fluorescence sensor for Fe{sup 3+} via reversible CHEF mechanism. • Distinct color changes “naked-eye” and rapid switch-on fluorescence detection for Fe{sup 3+}. • Detection limit was calculated to be 2.1 μM for Fe{sup 3+} ion.

  18. Fluorescence fluctuation of Rhodamine 6G dye for high repetition rate laser excitation

    International Nuclear Information System (INIS)

    Singh, Nageshwar; Patel, Hemant K.; Dixit, S.K.; Vora, H.S.

    2013-01-01

    In this paper, fluorescence from Rhodamine 6G dye for stationary and flowing liquid medium, excited by copper vapor laser, operating at 6 kHz pulse repetition frequency, was investigated. Large fluctuations in spectral width (about 5 nm) and spectral intensity in the fluorescence from stationary dye solution were observed, while fluctuations in the spectral width diminish in a flowing dye medium. However, this increases spectral intensity and slightly red shifts the fluorescence peak emission wavelength. Theoretical analysis was carried out to explain the observed results by incorporating the temperature induced refractive index, beam deflection and spectral variation in stationary dye solution. Numerical analysis of thermal load and contour of temperature in the optical pumped region inside the dye cell in stationary, 0.2 and 1.5 m/s flow velocity was also investigated to support our analysis. - Highlights: ► High repetition rate excitation generates inhomogeneity in the gain medium. ► Fluorescence of Rhodamine 6G in stationary and flowing medium was carried out. ► Fluorescence fluctuations lessen in flowing medium in contrast to stationary medium. ► Our theoretical and numerical analysis enlightens the experimented outcome trend.

  19. A rhodamine-labeled citalopram analogue as a high-affinity fluorescent probe for the serotonin transporter

    DEFF Research Database (Denmark)

    Zhang, Peng; Jørgensen, Trine Nygaard; Løland, Claus Juul

    2013-01-01

    A novel fluorescent ligand was synthesized as a high-affinity, high specificity probe for visualizing the serotonin transporter (SERT). The rhodamine fluorophore was extended from an aniline substitution on the 5-position of the dihydroisobenzofuran ring of citalopram (2, 1-(3-(dimethylamino......)propyl)-1-(4-fluorophenyl)-1,3-dihydroisobenzofuran-5-carbonitrile), using an ethylamino linker. The resulting rhodamine-labeled ligand 8 inhibited [3H]5-HT uptake in COS-7 cells (Ki = 225 nM) with similar potency to the tropane-based JHC 1-064 (1), but with higher specificity towards the SERT relative...

  20. Bright new colours: the history and analysis of fluorescein, eosin, erythrosine, rhodamine and some of their derivatives

    NARCIS (Netherlands)

    de Keijzer, M.; van Bommel, M.R.; Kirby, J.

    2017-01-01

    This paper discusses the history, chemical constitution, production, dyeing processes and properties of the hydroxyphthaleins. Important dyes of this dye class are fluorescein, the eosins, phloxines, erythrosines, rose bengals and rhodamines. These dyes were used for textile dyeing and as painting

  1. Novel synthesis and initial preclinical evaluation of (18)F-[FDG] labeled rhodamine: a potential PET myocardial perfusion imaging agent.

    Science.gov (United States)

    AlJammaz, Ibrahim; Al-Otaibi, Basim; AlHindas, Hussein; Okarvi, Subhani M

    2015-10-01

    Myocardial perfusion imaging is one of the most commonly performed investigations in nuclear medicine studies. Due to the clinical importance of [(18)F]-fluoro-2-deoxy-D-glucose ([(18)F]-FDG) and its availability in almost every PET center, a new radiofluorinated [(18)F]-FDG-rhodamine conjugate was synthesized using [(18)F]-FDG as a prosthetic group. In a convenient and simple one-step radiosynthesis, [(18)F]-FDG-rhodamine conjugate was prepared in quantitative radiochemical yields, with total synthesis time of nearly 20 min and radiochemical purity of greater than 98%, without the need for HPLC purification, which make these approaches amenable for automation. Biodistribution studies in normal rats at 60 min post-injection demonstrated a high uptake in the heart (>11% ID/g) and favorable pharmacokinetics. Additionally, [(18)F]-FDG-rhodamine showed an extraction value of 27.63%±5.12% in rat hearts. These results demonstrate that [(18)F]-FDG-rhodamine conjugate may be useful as an imaging agent for the positron emission tomography evaluation of myocardial perfusion. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. A Study on the Interaction of Rhodamine B with Methylthioadenosine Phosphorylase Protein Sourced from an Antarctic Soil Metagenomic Library

    Science.gov (United States)

    Bujacz, Anna; Wierzbicka-Woś, Anna; Kur, Józef

    2013-01-01

    The presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified and its UV-VIS absorbance spectrum and emission spectrum were assayed. Simultaneously, the same spectroscopic studies were carried out for rhodamine B in the absence or presence of RSFP protein or native E. coli proteins, respectively. The results of the spectroscopic studies suggested that the fluorescence of E. coli cells expressing rsfp gene under UV illumination is due to the interaction of rhodamine B molecules with the RSFP protein. Finally, this interaction was proved by a crystallographic study and then by site-directed mutagenesis of rsfp gene sequence. The crystal structures of RSFP apo form (1.98 Å) and complex RSFP/RB (1.90 Å) show a trimer of RSFP molecules located on the crystallographic six fold screw axis. The RSFP complex with rhodamine B revealed the binding site for RB, in the pocket located on the interface between symmetry related monomers. PMID:23383268

  3. A first principles study of fluorescence quenching in rhodamine B dimers : how can quenching occur in dimeric species?

    NARCIS (Netherlands)

    Setiawan, Dani; Kazaryan, Andranik; Martoprawiro, Muhamad Abdulkadir; Filatov, Michael

    2010-01-01

    Rhodamine B (RhB) is widely used in chemistry and biology due to its high fluorescence quantum yield. In high concentrations, the quantum yield of fluorescence decreases considerably which is attributed to the formation of RhB dimers. In the present work, a possible mechanism of fluorescence

  4. Chemiluminescence behavior based on oxidation reaction of rhodamine B with cerium(IV) in sulfuric acid medium

    Energy Technology Data Exchange (ETDEWEB)

    Ma Yongjun; Jin Xiaoyong; Zhou Min; Zhang Ziyu; Teng Xiulan; Chen Hui

    2003-08-18

    The chemiluminescence (CL) of the rhodamine B (RhB)-cerium(IV) system was investigated by flow-injection. Rhodamine B was suggested to be a suitable chemiluminescent reagent in acidic conditions. When the concentration of rhodamine B was 100 mg l{sup -1} and cerium sulfate was 1.6 mmol l{sup -1} in sulfuric acid, the chemiluminescent intensity was found to be highest by using 0.3 mol l{sup -1} sulfuric acid as a carrier solution. The particular chemiluminescent system could tolerate such distinct acidic environments that it was utilized for detecting many compounds that are stable in acidic solutions. Furthermore, by virtue of IR, UV-Vis and luminescence spectroscopic measurements, the chemiluminescent behavior of rhodamine B was studied and a possible mechanism for this chemiluminescent reaction was proposed. The emitter was affirmed to be a radical species due to one of the oxidation products of RhB; the chemiluminescent emissive wavelength was about 425 nm.

  5. Cavity-enhanced spontaneous emission rates for rhodamine 6-G in levitated microdroplets

    International Nuclear Information System (INIS)

    Barnes, M.D.; Whitten, W.B.; Ramsey, J.M.; Arnold, S.

    1992-01-01

    Fluorescence decay kinetics of Rhodamine 6-G molecules in levitated glycerol microdroplets (4--20 microns in diameter) have been investigated to determine the effects of spherical cavity resonances on spontaneous emission rates. For droplet diameters greater than 10 microns, the fluorescence lifetime is essentially the same as in bulk glycerol. As the droplet diameter is decreased below 10 microns, bi-exponential decay behavior is observed with a slow component whose rate is similar to bulk glycerol, and a fast component whose rate is as much as a factor of 10 larger than the bulk decay rate. This fast component is attributed to cavity enhancement of the spontaneous emission rate and, within the weak coupling approximation, a value for the homogeneous linewidth at room temperature can be estimated from the fluorescence lifetime data

  6. Rapid and simple preparation of rhodamine 6G loaded HY zeolite for highly selective nitrite detection

    Science.gov (United States)

    Viboonratanasri, Duangkamon; Pabchanda, Suwat; Prompinit, Panida

    2018-05-01

    In this study, a simple, rapid and relatively less toxic method for rhodamine 6G dye adsorption on hydrogen-form Y-type zeolite for highly selective nitrite detection was demonstrated. The adsorption behavior was described by Langmuir isotherm and the adsorption process reached the equilibrium promptly within a minute. The developed test papers characterized by fluorescence technique display high sensing performance with wide working range (0.04-20.0 mg L-1) and high selectivity. The test papers show good reproducibility with relative standard deviation (RSD) of 7% for five replicated determinations of 3 mg L-1 of nitrite. The nitrite concentration determined by using the test paper was in the same range as using ion chromatography within a 95% confidence level. The test papers offer advantages in terms of low cost and practical usage enabling them to be a promising candidate for nitrite sensor in environmental samples, food, and fertilizers.

  7. Two rhodamine 6G derivative compounds: a structural and fluorescence single-crystal study.

    Science.gov (United States)

    Di Paolo, Matias; Bossi, Mariano L; Baggio, Ricardo; Suarez, Sebastián A

    2016-10-01

    The synthesis, characterization, structural analysis and fluorescence properties of two rhodamine 6G derivatives are described, namely a propargylamine derivative, 3',6'-bis(ethylamino)-2',7'-dimethyl-2-(methylcyanide)spiro[isoindole-1,9'-xanthen]-3(2H)-one (I), and a γ-aminobutyric acid (GABA) derivative, 3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindole-1,9'-xanthen]-2(3H)-yl)butyricacid (II). Both structures are compared with four similar ones from the Cambridge Structural Database (CSD), and the interactions involved in the stabilization are analyzed using the atoms in molecules (AIM) theory. Finally, a single-crystal in-situ reaction study is presented, carried out by fluorescence methods, which enabled the `opening' of the spirolactam ring in the solid phase.

  8. Synthesis of a Cu2+-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging

    Directory of Open Access Journals (Sweden)

    Chunwei Yu

    2014-11-01

    Full Text Available A new fluorescent probe P based on rhodamine for Cu2+ was synthesized and characterized. The new probe P showed high selectivity to Cu2+ over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10−6–1.0 × 10−5 M with a detection limit of 3.3 × 10−7 M Cu2+ in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0. Furthermore, it has been used for imaging of Cu2+ in living cells with satisfying results.

  9. Efficient photocatalytic degradation of rhodamine 6G with a quantum dot-metal organic framework nanocomposite.

    Science.gov (United States)

    Kaur, Rajnish; Vellingiri, Kowsalya; Kim, Ki-Hyun; Paul, A K; Deep, Akash

    2016-07-01

    The hybrid structures of metal organic frameworks (MOFs) and nanoparticles may offer the realization of effective photocatalytic materials due to combined benefits of the porous and molecular sieving properties of MOF matrix and the functional characteristics of encapsulated nanoparticles. In this study, cadmium telluride (CdTe) quantum dots (QD) are conjugated with a europium-MOF for the synthesis of a novel nanocomposite material with photocatalytic properties. Successful synthesis of a QD/Eu-MOF nanocomposite was characterized with various spectroscopic and microscopic techniques. This QD/Eu-MOF is found to be an effective catalyst to complete the degradation of Rhodamine 6G dye within 50 min. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Highly Selective Deethylation of Rhodamine B on Prepared in Supercritical Fluids

    Directory of Open Access Journals (Sweden)

    Yuzun Fan

    2012-01-01

    Full Text Available Pure phase anatase TiO2 nanoparticles with sizes of 5–8 nm and varying crystallinity were synthesized in supercritical isopropanol/water using a continuous flow reactor. Their photodegradation of rhodamine B (RhB was evaluated under visible light irradiation. The as-prepared TiO2 nanoparticles show much higher photodegradation efficiencies than commercial Degussa P25 TiO2. Moreover, the photodegradation of RhB on the as-prepared TiO2 follows a different process from that on P25 TiO2, quicker N-deethylation and slower cleavage of conjugated chromophore structure. Based on PXRD, TEM, and BET measurements, these two photodegradation properties have been explained by the physicochemical properties of TiO2.

  11. Marine sponge skeleton photosensitized by copper phthalocyanine: A catalyst for Rhodamine B degradation

    Directory of Open Access Journals (Sweden)

    Norman Małgorzata

    2016-01-01

    Full Text Available We present a combined approach to photo-assisted degradation processes, in which a catalyst, H2O2 and UV irradiation are used together to enhance the oxidation of Rhodamine B (RB. The heterogeneous photocatalyst was made by the process of adsorption of copper phthalocyanine tetrasulfonic acid (CuPC onto purified spongin-based Hippospongia communis marine sponge skeleton (HcS. The product obtained, CuPC-HcS, was investigated by a variety of spectroscopic (carbon-13 nuclear magnetic resonance 13C NMR, Fourier transform infrared spectroscopy FTIR, energy-dispersive X-ray spectroscopy EDS and microscopic techniques (scanning electron microscopy SEM, fluorescent and optical microscopy, as well as thermal analysis. The study confirms the stable combination of the adsorbent and adsorbate. For a 10 mg/L RB solution, the percentage degradation reached 95% using CuPC-HcS as a heterocatalyst. The mechanism of RB removal involves adsorption and photodegradation simultaneously.

  12. Application of activated carbon derived from scrap tires for adsorption of Rhodamine B.

    Science.gov (United States)

    Li, Li; Liu, Shuangxi; Zhu, Tan

    2010-01-01

    Activated carbon derived from solid hazardous waste scrap tires was evaluated as a potential adsorbent for cationic dye removal. The adsorption process with respect to operating parameters was investigated to evaluate the adsorption characteristics of the activated pyrolytic tire char (APTC) for Rhodamine B (RhB). Systematic research including equilibrium, kinetics and thermodynamic studies was performed. The results showed that APTC was a potential adsorbent for RhB with a higher adsorption capacity than most adsorbents. Solution pH and temperature exert significant influence while ionic strength showed little effect on the adsorption process. The adsorption equilibrium data obey Langmuir isotherm and the kinetic data were well described by the pseudo second-order kinetic model. The adsorption process followed intra-particle diffusion model with more than one process affecting the adsorption process. Thermodynamic study confirmed that the adsorption was a physisorption process with spontaneous, endothermic and random characteristics.

  13. A new rhodamine-based fluorescent probe for the discrimination of Fe"3"+ from Fe"2"+

    International Nuclear Information System (INIS)

    You, Qi Hua; Huang, Hua Bin; Zhuang, Zhi Xia; Wang, Xiao Ru; Chan, Wing Hong

    2016-01-01

    A new rhodamine-based fluorescent probe for the discrimination of Fe"3"+ from Fe"2"+ has been designed and investigated. The probe shows an immediate visual color change in response to Fe"3"+ and Cu"2"+, while only Fe"3"+ triggers the fluorescent change of the probe. The existence of large amount of other metal ions shows negligible interference in the detection of Fe"3"+. The association constant K_a_s_s of 4.64 × 10"8 M"-"2 (R"2 = 0.994) and 5.38 × 10"8 M"-"2 (R"2 = 0.991) of the complex was derived from UV/Vis and fluorescence titration assuming 1:2 stoichiometry of probe–Fe"3"+ complex, respectively

  14. Studies of rhodamine-123: effect on rat prostate cancer and human prostate cancer cells in vitro.

    Science.gov (United States)

    Arcadi, J A; Narayan, K S; Techy, G; Ng, C P; Saroufeem, R M; Jones, L W

    1995-06-01

    The effect of the lipophilic, cationic dye, Rhodamine-123 (Rh-123), on prostate cancer in rats, and on three tumor cell lines in vitro is reported here. The general toxicity of Rh-123 in mice has been found to be minimal. Lobund-Wistar (L-W) rats with the autochthonous prostate cancer of Pollard were treated for six doses with Rh-123 at a dose of 15 mg/kg subcutaneously every other day. Microscopic examination of the tumors revealed cellular and acinar destruction. The effectiveness of Rh-123 as a cytotoxic agent was tested by clonogenic and viability assays in vitro with three human prostate cancer cell lines. Severe (60-95%) growth inhibition was observed following Rh-123 exposure for 2-5 days at doses as low as 1.6 micrograms/ml in all three prostate cancer cell lines.

  15. Two sugar-rhodamine "turn-on" fluorescent probes for the selective detection of Fe3 +

    Science.gov (United States)

    Chen, Qing; Fang, Zhijie

    2018-03-01

    Two new sugar-rhodamine fluorescent probes (RDG1 and RDG2) have been synthesized and characterized by 1H NMR, 13C NMR and HRMS. Their UV-Vis, fluorescence spectra and fluorescence-response to Fe3 + are investigated and discussed. RDG1 had a very nice linear relationship between UV absorbance and Fe3 + concentration with the correlation coefficient as high as 0.997 and the detection limit is 3.46 × 10- 6 M. Upon the addition of Fe3 +, the spirolactam ring of RDG1 was opened and a 1:1 metal ligand complex was formed from Job's plot. The results showed that RDG1 can be used as an effective fluorescent probe for selective detection of Fe3 + in water. RDG2 was incorporated the well-known rhodamine group and a water-soluble D-glucose group within one molecule and can be used for detecting Fe3 + in natural water as a selective fluorescent sensor. The addition of Fe3 + into RDG2 resulted in a strongly enhanced fluorescence as well as color change of solution from colorless to pink. Job's plot of RDG2 indicated 1:1 stoichiometry of RDG2-Fe3 +. RDG2 can serve as a probe for Fe3 + between pH = 4.0 to 7.0 and it's detection limit is 2.09 × 10- 6 M. The OFF-ON fluorescent mechanisms of RDG1-Fe3 + and RDG2-Fe3 + are proposed.

  16. A specific Tween-80-Rhodamine S-MWNTs phosphorescent reagent for the detection of trace calcitonin

    Energy Technology Data Exchange (ETDEWEB)

    Liu Jiaming, E-mail: zzsyliujiaming@163.com [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Huang Xiaomei; Zhang Lihong; Zheng Zhiyong [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou, 363000 (China); Lin Xuan; Zhang Xiaoyang; Jiao Li; Cui Malin [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Jiang Shulian [Fujian Provincial Bureau of Quality and Technical Supervision, Zhangzhou, 363000 (China); Lin Shaoqin [Department of Biochemistry, Fujian Education College, Fuzhou 350001 (China)

    2012-09-26

    Graphical abstract: A new Tween-80-Rhodamine S-water-soluble multi-walled carbon nanotubes (Tween-80-Rhod.S-MWNTs-EDC-NHS, TRMEN) phosphorescent labelling reagent was developed. High sensitive solid substrate room temperature phosphorescence immunoassay (SSRTPIA) for the determination of calcitonin (CT) in human serum and the prediction of human diseases based on the TRMEN could be used to label anti-calcitonin antibody (Ab{sub CT}) to form the TRMEN-Ab{sub CT} labelling product, which could take high specific immunoreaction with CT causing that the {Delta}I{sub p} of the system was linear to the content of CT. Moreover, the reaction mechanisms of both labelling Ab{sub CT} by TRMEN and SSRTPIA for the determination of trace CT were discussed. This research not only provides a new hormones analysis method, but also expands the application field of MWNTs and promotes the development of SSRTP and IA. --Highlights: Black-Right-Pointing-Pointer A Tween-80-Rhodamine S-multi-walled carbon nanotubes labelling reagent was developed. Black-Right-Pointing-Pointer The phosphorescence immunoassay was established for the determination of calcitonin. Black-Right-Pointing-Pointer This method has been applied to determine CT and the prediction of diseases. Black-Right-Pointing-Pointer The structure of MWNTs was characterized with SEM and IR. Black-Right-Pointing-Pointer The mechanisms for both determining trace CT and labelling Ab{sub CT} were discussed. - Abstract: The present study proposed a simple sensitive and specific immunoassay for the quantification of calcitonin (CT) in human serum with water-soluble multi-walled carbon nanotubes (MWNTs). The -COOH group of MWNTs could react with the -NH- group of rhodamine S (Rhod.S) molecules to form Rhod.S-MWNTs, which could emit room temperature phosphorescence (RTP) on acetate cellulose membrane (ACM) and react with Tween-80 to form micellar compound. Tween-80-Rhod.S-MWNTs (TRM), as a phosphorescent labelling reagent, could

  17. Development of a high-throughput liquid state assay for lipase activity using natural substrates and rhodamine B.

    Science.gov (United States)

    Zottig, Ximena; Meddeb-Mouelhi, Fatma; Beauregard, Marc

    2016-03-01

    A fluorescence-based assay for the determination of lipase activity using rhodamine B as an indicator, and natural substrates such as olive oil, is described. It is based on the use of a rhodamine B-natural substrate emulsion in liquid state, which is advantageous over agar plate assays. This high-throughput method is simple and rapid and can be automated, making it suitable for screening and metagenomics application. Reaction conditions such as pH and temperature can be varied and controlled. Using triolein or olive oil as a natural substrate allows monitoring of lipase activity in reaction conditions that are closer to those used in industrial settings. The described method is sensitive over a wide range of product concentrations and offers good reproducibility. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. Efficient Photocatalytic Degradation of Rhodamine B Dye by Aligned Arrays of Self-Assembled Hydrogen Titanate Nanotubes

    Directory of Open Access Journals (Sweden)

    Sriparna Chatterjee

    2014-01-01

    Full Text Available We show that an aligned array of hydrothermally grown, multiwalled hydrogen titanate (H2Ti3O7 nanotubes—anchored to both faces of a metallic Ti foil—acts as an efficient photocatalyst. We studied the degradation of rhodamine B dye in the presence of the nanostructured photocatalyst under UV irradiation, by monitoring the optical absorption of the dye. Rhodamine B was chosen as a representative—and particularly harmful—industrial pollutant dye. The inner and outer diameters of the H2Ti3O7 nanotubes were 5 nm and 10 nm, respectively. The nanotube array catalyst is recyclable and structurally stable. Most importantly, it shows comparable or higher photodecomposition rate constant than those of both H2Ti3O7 nanotube powder and P-25 (Degussa. The enhanced photocatalytic performance may be ascribed to the nanotube array having a superhydrophilic surface with a high accessible surface area.

  19. Synthesis of metal free ultrathin graphitic carbon nitride sheet for photocatalytic dye degradation of Rhodamine B under visible light irradiation

    Science.gov (United States)

    Rahman, Shakeelur; Momin, Bilal; Higgins M., W.; Annapure, Uday S.; Jha, Neetu

    2018-04-01

    In recent times, low cost and metal free photocatalyts driven under visible light have attracted a lot of interest. One such photo catalyst researched extensively is bulk graphitic carbon nitride sheets. But the low surface area and weak mobility of photo generated electrons limits its photocatalytic performance in the visible light spectrum. Here we present the facile synthesis of ultrathin graphitic carbon nitride using a cost effective melamine precursor and its application in highly efficient photocatalytic dye degradation of Rhodamine B molecules. Compared to bulk graphitic carbon nitride, the synthesized ultrathin graphitic carbon nitride shows an increase in surface area, a a decrease in optical band gap and effective photogenerated charge separation which facilitates the harvest of visible light irradiation. Due to these optimal properties of ultrathin graphitic carbon nitride, it shows excellent photocatalytic activity with photocatalytic degradation of about 95% rhodamine B molecules in 1 hour.

  20. Random laser emission from a Rhodamine B-doped GPTS/TEOS-derived organic/silica monolithic xerogel

    Science.gov (United States)

    Abegão, Luis M. G.; Manoel, D. S.; Otuka, A. J. G.; Ferreira, P. H. D.; Vollet, D. R.; Donatti, D. A.; De Boni, L.; Mendonça, C. R.; De Vicente, F. S.; Rodrigues, J. J., Jr.; Alencar, M. A. R. C.

    2017-06-01

    A Rhodamine B-doped 3-glycidoxypropyltrimethoxysilane (GPTS)/tetraethyl orthosilicate (TEOS)-derived organic/silica monolithic xerogel with excellent optical properties was prepared and its potential as a random laser host investigated. This hybrid material has a non-porous organic/inorganic morphology with silica-rich nanoparticles of less than 10 nm in diameter homogeneously dispersed within the matrix. Random laser emission with incoherent feedback, centered at 618 nm, was observed from Rhodamine B incorporated into the monolithic xerogel when excited by a 532 nm pulsed laser. This hybrid system is shown to be very promising for the development of a new class of random laser-based integrated devices, with applications ranging from optical bio-imaging to sensing.

  1. Modification of fluorescence and optical properties of Rhodamine B dye doped PVA/Chitosan polymer blend films

    Science.gov (United States)

    Padmakumari, R.; Ravindrachary, V.; Mahantesha, B. K.; Sagar, Rohan N.; Sahanakumari, R.; Bhajantri, R. F.

    2018-05-01

    Pure and Rhodamine B doped Poly (vinyl alcohol)/Chitosan composite films are prepared using solution casting method. Fourier transforms infrared spectra (FTIR), Ultraviolet-Visible (UV-Vis), fluorescence studies were used to characterize the prepared polymer films. The FT-IR results show that the appearance of new peaks along with shift in peak positions indicates the interaction of Rhodamine B with PVA-CS blend. Optical absorption edge, band gap and activation energy were determined from UV-Visible studies. The optical absorption edge increases, band gap decreases and activation energy increases with dopant concentration respectively. The corresponding emission spectra were studied using fluorescence spectroscopy. From the fluorescence study the quenching phenomena are observed in emission wavelength range of 607nm-613nm upon excitation with absorption maxima 443nm.

  2. Study of the deposition features of the organic dye Rhodamine B on the porous surface of silicon with different pore sizes

    Energy Technology Data Exchange (ETDEWEB)

    Lenshin, A. S., E-mail: lenshinas@phys.vsu.ru; Seredin, P. V.; Kavetskaya, I. V.; Minakov, D. A.; Kashkarov, V. M. [Voronezh State University (Russian Federation)

    2017-02-15

    The deposition features of the organic dye Rhodamine B on the porous surface of silicon with average pore sizes of 50–100 and 100–250 nm are studied. Features of the composition and optical properties of the obtained systems are studied using infrared and photoluminescence spectroscopy. It is found that Rhodamine-B adsorption on the surface of por-Si with various porosities is preferentially physical. The optimal technological parameters of its deposition are determined.

  3. Solvent extraction of W(VI) and Hg(II) with malachite green and rhodamine-B respectively into organic solvents

    International Nuclear Information System (INIS)

    Patil, V.B.; David, M.M.; Turel, Z.R.

    1992-01-01

    Aqueous malachite green and alcoholic rhodamine-B have been used for the extraction of tungsten( W(VI)) and mercury( Hg(II)) respectively into nitrobenzene. This paper deals with developing a rapid method and selective method for the extraction of tungsten(W(VI)) and mercury (Hg(II)) using malachite green and rhodamine-B respectively. 185 W and 203 Hg were used as tracers for studying the extraction process.(author). 2 refs., 2 tab

  4. Rhodamine B triggers ovarian toxicity through oxidative stress, decreases in the number of follicles, 17B-estradiol level, and thickness of endometrium

    Directory of Open Access Journals (Sweden)

    Syiska Atik Maryanti

    2014-06-01

    Full Text Available Objective: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17beta-estradiol and thickness of endometrium. Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P 0.05. Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P 0.05. Administration of rhodamine B of the second and third doses in female rats can reduce 17-estradiol level significantly compared to the control (P 0.05. The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P 0.05. Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17-estradiol which ultimately lowered the thickness of endometrium. [Cukurova Med J 2014; 39(3.000: 451-457

  5. Lasing of a Solid-State Active Element Based on Anodized Aluminum Oxide Film Doped with Rhodamine 6G

    Science.gov (United States)

    Shelkovnikov, V. V.; Lyubas, G. A.; Korotaev, S. V.; Kopylova, T. N.; Tel'minov, E. N.; Gadirov, R. M.; Nikonova, E. N.; Nikonov, S. Yu.; Solodova, T. A.; Novikov, V. A.

    2017-04-01

    Spectral-luminescent and lasing characteristics of rhodamine 6G in porous aluminum oxide films anodized under various conditions are investigated. Lasing is obtained without external resonator in the longitudinal scheme under excitation by the second harmonic of Nd3+:YAG-laser radiation. The threshold pump power densities are in the range 3.5-15 MW/cm2 depending on the anodizing conditions. Wherein, the lasing line narrows down from 12 to 5 nm.

  6. Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells

    Czech Academy of Sciences Publication Activity Database

    Cmiel, V.; Skopalík, J.; Poláková, K.; Solař, J.; Havrdová, M.; Milde, D.; Justan, I.; Magro, M.; Starčuk jr., Zenon; Provazník, I.

    2017-01-01

    Roč. 46, JUL (2017), s. 433-444 ISSN 0175-7571 R&D Projects: GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01 Institutional support: RVO:68081731 Keywords : intracellular fluorescent labels * stem cell tracking * dual contrast agents * iron oxide nanoparticles * confocal microscopy * mesenchymal stromal cells * rhodamine Subject RIV: FS - Medical Facilities ; Equipment OBOR OECD: Biophysics Impact factor: 1.472, year: 2016

  7. Nanobeads of zinc oxide with rhodamine B dye as a sensitizer for dye sensitized solar cell application

    Energy Technology Data Exchange (ETDEWEB)

    Baviskar, P.K. [Thin Film and Nano Science Laboratory, Department of Physics, School of Physical Sciences, North Maharashtra University, Jalgaon 425 001, MS (India); Zhang, J.B. [Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (China); Gupta, V.; Chand, S. [Organic and Hybrid Solar Cell, Physics of Energy Harvesting Division, Dr. K. S. Krishnan Marg, National Physical Laboratory, New Delhi 110012 (India); Sankapal, B.R., E-mail: brsankapal@rediffmail.com [Thin Film and Nano Science Laboratory, Department of Physics, School of Physical Sciences, North Maharashtra University, Jalgaon 425 001, MS (India)

    2012-01-05

    Highlights: > Synthesis of ZnO film was done at room temperature (27 deg. C). > Simple and inexpensive chemical bath deposition method was employed. > The as deposited film consists of mixed phases of hydroxide and oxide. > The post annealing was done at 200 deg. C in order to remove hydroxide phase. > Low-cost, metal free Rhodamine B dye was used for DSSC application. - Abstract: Cost effective, ruthenium metal free rhodamine B dye has been chemically adsorbed on ZnO films consisting of nanobeads to serve as a photo anode in dye sensitized solar cells. These ZnO films were chemically synthesized at room temperature (27 deg. C) on to fluorine doped tin oxide (FTO) coated glass substrates followed by annealing at 200 deg. C. These films consisting of inter connected nanobeads (20-40 nm) which are due to the agglomeration of very small size particles (3-5 nm) leading to high surface area. The film shows wurtzite structure having high crystallinity with optical direct band gap of 3.3 eV. Optical absorbance measurements for rhodamine B dye covered ZnO film revealed the good coverage in the visible region (460-590 nm) of the solar spectrum. With poly-iodide liquid as an electrolyte, device exhibits photon to electric energy conversion efficiency ({eta}) of 1.26% under AM 1.5G illumination at 100 mW/cm{sup 2}.

  8. Surface Behavior of Rhodamin and Tartrazine on Silica-Cellulose Sol-Gel Surfaces by Thin Layer Elution

    Directory of Open Access Journals (Sweden)

    Surjani Wonorahardjo

    2016-05-01

    Full Text Available Physical and chemical interactions are the principles for different types of separation systems as the equillibrium dynamics on surface plays a key-role. Surface modification is a way for selective separation at interfaces. Moreover, synthesis of gel silica by a sol-gel method is preferred due to the homogeneity and surface feature easily controlled. Cellulose can be added in situ to modified the silica features during the process. Further application for to study interaction of rhodamin and tartrazine in its surface and their solubilities in mobile phase explains the possibility for their separation. This paper devoted to evaluate the surface behavior in term of adsorption and desorption of tartrazine and rhodamin on silica-cellulose thin layer in different mobile phase. Some carrier liquids applied such as methanol, acetone, n-hexane and chloroform. The result proves tartrazine and rhodamin is separated and have different behavior in different mobile phase. The retardation factors (Rf of the mixtures suggest complexity behavior on silica-cellulose surface.

  9. Removal of methylene blue and rhodamine B from water by zirconium oxide/graphene

    Directory of Open Access Journals (Sweden)

    Sumita Rani

    2016-04-01

    Full Text Available Methylene blue (MB and rhodamine B dyes (RB were degraded from water using zirconium oxide (ZrO2 and zirconium oxide/graphene composites (ZrO2/GR as photocatalyst. The photocatalytic efficiency was calculated from absorption spectra obtained using UV–visible spectroscopy. It has been observed that photodegradation time as well as photocatalytic efficiency increase with the concentration of catalyst up to a certain limit after which effect was reversed. The degradation was studied as a function of pH also. It was found that photocatalytic efficiency was more in alkaline medium than acidic medium. Degradation of RB takes place at higher value of pH as compared to MB. The degradation time for MB was 1 h using ZrO2 which get reduced to 32 min using ZrO2/GR composite and for RB it reduced to 40 min (using ZrO2/GR from 80 min (ZrO2.

  10. Rhodamine Inhibitors of P-glycoprotein: An Amide/Thioamide “Switch” for ATPase Activity

    Science.gov (United States)

    Gannon, Michael K.; Holt, Jason J.; Bennett, Stephanie M.; Wetzel, Bryan R.; Loo, Tip W.; Bartlett, M. Claire; Clarke, David M.; Sawada, Geri A.; Higgins, J. William; Tombline, Gregory; Raub, Thomas J.; Detty, Michael R.

    2012-01-01

    We have examined 46 tetramethylrosamine/rhodamine derivatives with structural diversity in the heteroatom of the xanthylium core, the amino substituents of the 3- and 6-positions, and the alkyl, aryl, or heteroaryl group at the 9-substituent. These compounds were examined for affinity and ATPase stimulation in isolated MDR3 CL P-gp and human P-gp-His10, for their ability to promote uptake of calcein AM and vinblastine in multidrug-resistant MDCKII-MDR1 cells, and for transport in monolayers of MDCKII-MDR1 cells. Thioamide 31-S gave KM of 0.087 μM in human P-gp. Small changes in structure among this set of compounds affected affinity as well as transport rate (or flux) even though all derivatives examined were substrates for P-gp. With isolated protein, tertiary amide groups dictate high affinity and high stimulation while tertiary thioamide groups give high affinity and inhibition of ATPase activity. In MDCKII-MDR1 cells, the tertiary thioamide-containing derivatives promote uptake of calcein AM and have very slow passive, absorptive, and secretory rates of transport relative to transport rates for tertiary amide-containing derivatives. Thioamide 31-S promoted uptake of calcein AM and inhibited efflux of vinblastine with IC50’s of ~2 μM in MDCKII-MDR1 cells. PMID:19402665

  11. Occurrence of rhodamine B contamination in capsicum caused by agricultural materials during the vegetation process.

    Science.gov (United States)

    Gao, Wei; Wu, Naiying; Du, Jingjing; Zhou, Li; Lian, Yunhe; Wang, Lei; Liu, Dengshuai

    2016-08-15

    This paper reports on the environmental rhodamine B (RhB) contamination in capsicum caused by agricultural materials during the vegetation process. Ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was applied to detect 64 capsicum samples from China, Peru, India and Burma. Results demonstrated that RhB was found in all samples at low concentrations (0.11-0.98 μg/kg), indicating RhB contamination in capsicums is probably a ubiquitous phenomenon. In addition, studies into soils, roots, stems and leaves in Handan of Hebei province, China showed that the whole ecologic chain had been contaminated with RhB with the highest levels in leaves. The investigation into the agricultural environment in Handan of Hebei province and Korla of Xinjiang province, China demonstrated that the appearances of RhB contamination in the tested capsicums are mainly due to the agricultural materials contamination. The study verified that environmental contamination should be an important origin for the RhB contamination in capsicum fruits. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Animal Bone Supported SnO2 as Recyclable Photocatalyst for Degradation of Rhodamine B Dye.

    Science.gov (United States)

    Wu, Yun; Wang, Hui; Cao, Mengdie; Zhang, Yichi; Cao, Feifei; Zheng, Xinsheng; Hu, Jinfei; Dong, Jiangshan; Xiao, Zhidong

    2015-09-01

    SnO2 nanoparticles supported on an animal bone which serves as inexpensive and environment-friendly natural products were developed by a facile hydrothermal approach. As a promising photocatalyst, the novel SnO2/porcine bone material exhibited high photocatalytic activity towards the degradation of rhodamine B (RhB) dye under UV-Vis irradiation. About 97.3% of RhB can be effectively decomposed by the catalysis with the SnO2/porcine bone in 90 min, while only 51.5% of RhB can be degraded by pure SnO2 nanoparticles. Moreover, the photocatalytic activity was incremental with the increase of cycle times in previous five cycles. It is mainly because the photocatalyst which has been used for several times possesses a stronger ability of light absorption and utilization compared to the fresh catalyst according to the results of the characterization and relative experiments. It is noteworthy that the animal bone support can improve the activity for the photocatalyst, which would provide further impetus to alternate synthesis strategies for photocatalysts and make the photocatalysis process faster, less expensive, and more environmentally friendly.

  13. Interaction of Lysozyme with Rhodamine B: A combined analysis of spectroscopic & molecular docking.

    Science.gov (United States)

    Millan, Sabera; Satish, Lakkoji; Kesh, Sandeep; Chaudhary, Yatendra S; Sahoo, Harekrushna

    2016-09-01

    The interaction of Rhodamine B (RB) with Lysozyme (Lys) was investigated by different optical spectroscopic techniques such as absorption, fluorescence, and circular-dichroism (CD), along with molecular docking studies. The fluorescence results (including steady-state and time-resolved mode) revealed that the addition of RB effectively causes strong quenching of intrinsic fluorescence in Lysozyme and mostly, by the static quenching mechanism. Different binding and thermodynamic parameters were calculated at different temperatures and the binding constant value was found to be 2963.54Lmol(-1) at 25°C. The average distance (r0) was found to be 3.31nm according to Förster's theory of non-radiative energy transfer between Lysozyme and RB. The conformational change in Lysozyme during interaction with RB was confirmed from absorbance, synchronous fluorescence, and circular dichroism measurements. Finally, molecular docking studies were done to confirm that the dye binds with Lysozyme. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Single bead near-infrared random laser based on silica-gel infiltrated with Rhodamine 640

    Science.gov (United States)

    Moura, André L.; Barbosa-Silva, Renato; Dominguez, Christian T.; Pecoraro, Édison; Gomes, Anderson S. L.; de Araújo, Cid B.

    2018-04-01

    Photoluminescence properties of single bead silica-gel (SG) embedded with a laser-dye were studied aiming at the operation of near-infrared (NIR) Random Lasers (RLs). The operation of RLs in the NIR spectral region is especially important for biological applications since the optical radiation has deep tissue penetration with negligible damage. Since laser-dyes operating in the NIR have poor stability and are poor emitters, ethanol solutions of Rhodamine 640 (Rh640) infiltrated in SG beads were used. The Rh640 concentrations in ethanol varied from 10-5 to 10-2 M and the excitation at 532 nm was made by using a 7 ns pulsed laser. The proof-of-principle RL scheme herein presented was adopted in order to protect the dye-molecules from the environment and to favor formation of aggregates. The RL emission from ≈650 nm to 720 nm, beyond the typical Rh640 monomer and dimer wavelengths emissions range, was attributed to the trade-off between reabsorption and reemission processes along the light pathways inside the SG bead and the contribution of Rh640 aggregates.

  15. Bifunctional Rhodamine Probes of Myosin Regulatory Light Chain Orientation in Relaxed Skeletal Muscle Fibers

    Science.gov (United States)

    Brack, Andrew S.; Brandmeier, Birgit D.; Ferguson, Roisean E.; Criddle, Susan; Dale, Robert E.; Irving, Malcolm

    2004-01-01

    The orientation of the regulatory light chain (RLC) region of the myosin heads in relaxed skinned fibers from rabbit psoas muscle was investigated by polarized fluorescence from bifunctional rhodamine (BR) probes cross-linking pairs of cysteine residues introduced into the RLC. Pure 1:1 BR-RLC complexes were exchanged into single muscle fibers in EDTA rigor solution for 30 min at 30°C; ∼60% of the native RLC was removed and stoichiometrically replaced by BR-RLC, and >85% of the BR-RLC was located in the sarcomeric A-bands. The second- and fourth-rank order parameters of the orientation distributions of BR dipoles linking RLC cysteine pairs 100-108, 100-113, 108-113, and 104-115 were calculated from polarized fluorescence intensities, and used to determine the smoothest RLC orientation distribution—the maximum entropy distribution—consistent with the polarized fluorescence data. Maximum entropy distributions in relaxed muscle were relatively broad. At the peak of the distribution, the “lever” axis, linking Cys707 and Lys843 of the myosin heavy chain, was at 70–80° to the fiber axis, and the “hook” helix (Pro830–Lys843) was almost coplanar with the fiber and lever axes. The temperature and ionic strength of the relaxing solution had small but reproducible effects on the orientation of the RLC region. PMID:15041671

  16. Adsorption kinetics of Rhodamine-B on used black tea leaves

    Directory of Open Access Journals (Sweden)

    Hossain Mohammad

    2012-08-01

    Full Text Available Abstract Rhodamine B (Rh-B is one of the most common pollutants in the effluents of textile industries effluents in developing countries. This study was carried out to evaluate the applicability of used black tea leaves (UBTL for the adsorptive removal of Rh-B from aqueous system by investigating the adsorption kinetics in batch process. The effects of concentration and temperature on adsorption kinetics were examined. First-, second- and pseudo-second order kinetic equations were used to investigate the adsorption mechanism. The adsorption of Rh-B on UBTL followed pseudo-second order kinetics. The equilibrium amount adsorbed and the equilibrium concentration were calculated from pseudo-second-order kinetic plots for different initial concentrations of Rh-B to construct the adsorption isotherm. The adsorption isotherm was well expressed by Langmuir equation. The maximum adsorption capacity of UBTL to Rh-B was found to be 53.2 mg/g at pH = 2.0. The equilibrium amount adsorbed, calculated from pseudo-second-order kinetic plots, increased with temperature increase. The positive value of enthalpy of adsorption, ΔHads = 31.22 kJ/mol, suggested that the adsorption of Rh-B on UBTL at pH = 2.0 is an endothermic process.

  17. Investigation of adsorption of Rhodamine B onto a natural adsorbent Argemone mexicana.

    Science.gov (United States)

    Khamparia, Shraddha; Jaspal, Dipika

    2016-12-01

    The present study aims at exploring the potential of the seeds of a tropical weed, Argemone mexicana (AM), for the removal of a toxic xanthene textile dye, Rhodamine B (RHB), from waste water. Impact of pH, adsorbent dosage, particle size, contact time and dye concentration have been assessed during adsorption. The weed has been well characterized by several latest techniques thereby providing an indepth information of the mechanism during adsorption. About 80% removal has been attained with 0.06 g of adsorbent over the studied system. Thermodynamic and kinetic studies, followed by second order kinetic model, directed towards the endothermic nature of adsorption. The results obtained from batch experiments were modelled using Langmuir and Freundlich isotherm and were analysed on the basis of R 2 and six error functions for selection of appropriate model. Langmuir isotherm was found to be best fitted to the experimental data with high values of R 2 and lower values of error functions. Adsorption study revealed the affinity of AM seeds for the dye ions present in waste water, introducing a novel adsorbent in field of waste water treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Formulation and in vitro interaction of rhodamine-B loaded PLGA nanoparticles with cardiac myocytes.

    Directory of Open Access Journals (Sweden)

    Antranik Jonderian

    2016-12-01

    Full Text Available This study aims to characterize rhodamine B (Rh B loaded poly(D,L-lactide-co-glycolide (PLGA nanoparticles (NPs and their interactions with cardiac myocytes. PLGA NPs were formulated using single emulsion solvent evaporation technique. The influence of varying parameters such as the stabilizer concentration, the sonication time, and the organic to aqueous ratio were investigated. The diameter, the dispersity, the encapsulation efficiency and the zeta potential of the optimized nanoparticles were about 184 nm, 0.19, 40% and -21.7 mV respectively. In vitro release showed that 29% of the Rh B was released within the first 8 hours. Scanning electron microscopy (SEM measurements performed on the optimized nanoparticles showed smooth surface and spherical shapes. No significant cytotoxic or apoptotic effects were observed on fetal cardiac myocytes after 24 and 48 hours of exposure with concentrations up to 200 µg/mL. The kinetic of the intracellular uptake was confirmed by confocal microscopy and cells took up PLGA NPs within the first hours. Interestingly, our data show an increase in the nanoparticles’ uptake with time of exposure. Taken together, we demonstrate for the first time that the designed NPs can be used as potential probes for drug delivery in cardiac myocytes.

  19. Adsorption of rhodamine B by acid activated carbon-Kinetic, thermodynamic and equilibrium studies

    Directory of Open Access Journals (Sweden)

    Shanmugam Arivoli

    2009-08-01

    Full Text Available A carbonaceous adsorbent prepared from an indigenous waste by acid treatment was tested for its efficiency in removing Rhodamine B (RDB. The parameters studied include agitation time, initial dye concentration, carbon dose, pH and temperature. The adsorption followed first order kinetics and the rate is mainly controlled by intra-particle diffusion. Freundlich and Langmuir isotherm models were applied to the equilibrium data. The adsorption capacity (Qm obtained from the Langmuir isotherm plots were 40.161, 35.700, 38.462 and 37.979 mg/g respectively at an initial pH of 7.0 at 30, 40, 50 and 60 0C. The temperature variation study showed that the RDB adsorption is endothermic and spontaneous with increased randomness at the solid solution interface. Significant effect on adsorption was observed on varying the pH of the RDB solutions. Almost 85% removal of RDB was observed at 60 0C. The Langmuir and Freundlich isotherms obtained, positive ?H0 value, pH dependent results and desorption of dye in mineral acid suggest that the adsorption of RDB by Banana bark carbon involves physisorption mechanism.

  20. A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

    International Nuclear Information System (INIS)

    Kempahanumakkagaari, Suresh Kumar; Thippeswamy, Ramakrishnappa; Malingappa, Pandurangappa

    2014-01-01

    A simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu 2+ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580 nm and displayed color change from colorless to pink for Cu 2+ in the pH range 1–6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu 2+ ions in living cells. This probe can detect Cu 2+ with good linear relationships from 10 to 100 nM with r=0.99971 then limit of detection was found to be 0.015 nM with ±0.91% RSD at 10 nM concentrations. -- Highlights: • The new sensitive, highly selective fluorescent chemodosimeter for Cu 2+ based on spirolactam ring opening process has been proposed. • The probe posse’s excellent cell permeability and it has been applied for fluorescence imaging of Cu 2+ ions in MCF-7 cell lines. • The protocol has been successfully applied for copper determination in blood and urine samples

  1. Xanthium strumarium L. seed hull as a zero cost alternative for Rhodamine B dye removal.

    Science.gov (United States)

    Khamparia, Shraddha; Jaspal, Dipika Kaur

    2017-07-15

    Treatment of polluted water has been considered as one of the most important aspects in environmental sciences. Present study explores the decolorization potential of a low cost natural adsorbent Xanthium strumarium L. seed hull for the adsorption of a toxic xanthene dye, Rhodamine B (RHB). The characterization of the adsorbent revealed the presence of high amount of carbon, when exposed to Electron Dispersive Spectroscopy (EDS). Further appreciable decolorization took place which was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) analysis noticing shift in peaks. Isothermal studies indicated multilayer adsorption following Freundlich isotherm. The rate of adsorption was supported by second order kinetics directing a chemical phenomenon during the process with dominance of film diffusion as the rate governing step. Moreover paper aims at correlating the chemical arena to the mathematical aspect providing an in-depth information of the studied treatment process. For proper assessment and validation of the observed data, experimental data has been statistically treated by applying different error functions namely, Chi-square test (χ 2 ), Sum of absolute errors (EABS) and Normalized standard deviation (NSD). Further practical applicability of the low cost adsorbent was evaluated by continuous column mode studies with 72.2% of dye recovery. Xanthium strumarium L. proved to be environment friendly low cost natural adsorbent for decolorizing RHB from aquatic system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Narrow Bandwidth Top-Emitting OLEDs Designed for Rhodamine 6G Excitation in Biological Sensing Applications

    Directory of Open Access Journals (Sweden)

    Matthias Jahnel

    2015-11-01

    Full Text Available Organic light emitting diodes (OLED are promising candidates offering in optical sensor applications to detect different gas compositions and excitable optical marker groups in chemical and biological processes. They enable attractive solutions for monitoring the gas phase composition of e.g., dissolved molecular oxygen (O2 species in bio reactors or excitation of fluorescent markers. In this work, we investigate different OLED devices for biomedical applications to excite the fluorescent dye rhodamine 6G (R6G. The OLED devices are built in top emission geometry comprising a distributed Bragg reflector (DBR acting as optical mirror. The OLED is optimized to provide a very narrow emission characteristic to excite the R6G at 530 nm wavelength and enabling the possibility to minimize the optical crosstalk between the OLED electroluminescence and the fluorescence of R6G. The DBR includes a thin film encapsulation and enables the narrowing of the spectral emission band depending on the number of DBR pairs. The comparison between optical simulation data and experimental results exhibits good agreement and proves process stability.

  3. Studies on the Removal of Rhodamine B and Malachite Green from Aqueous Solutions by Activated Carbon

    Directory of Open Access Journals (Sweden)

    A. Edwin Vasu

    2008-01-01

    Full Text Available Activated carbon prepared from tamarind fruit shells by direct carbonization was used for the removal of rhodamine B and malachite green dyes from aqueous solutions. Adsorption studies were performed by varying such parameters as dye concentration, pH of the dye solution, time and temperature. The equilibrium adsorption data obtained were used to calculate the Freundlich, Langmuir and Redlich-Peterson isotherm parameters. Increase in pH of the solution pH resulted in increased adsorption of both the dyes. Kinetic studies indicate that the pseudo-second order model can be used for describing the dynamics of the sorption processes. Film diffusion of the dyes was the rate determining step at low dye concentrations while diffusion of dyes through the pores the carbon particles determined the overall uptake at high concentrations. Thermodynamic parameters of the endothermic sorptions were evaluated using van’t Hoff equation. Desorption studies with acids were also performed in order to regenerate the used carbons.

  4. Determination of cyanide using a chemiluminescence system composed of permanganate, rhodamine B, and gold nanoparticles

    International Nuclear Information System (INIS)

    Amjadi, Mohammad; Hassanzadeh, Javad; Manzoori, Jamshid L.

    2014-01-01

    We describe a new chemiluminescence (CL) system based on the oxidation of rhodamine B (RhoB) with alkaline potassium permanganate in the presence of gold nanoparticles (Au-NPs) and anionic detergent sodium dodecyl sulfate. Free RhoB is weakly chemiluminescent when oxidized with permanganate at alkaline pH values. However, a remarkably strong enhancement of CL is observed in the presence of Au-NPs, probably due to a strong interaction between RhoB and the NPs. The possible mechanism was studied via recording the CL emission. It is also found that the intensity of CL gradually decreases in the presence of cyanide due to its interaction with the Au-NPs. The relation between the decreased CL intensity and cyanide concentration was exploited to develop a method for the determination of cyanide in the 0.01–0.5 μM concentration range, with a detection limit of 2.8 nM. The method was used to determine cyanide in spiked water, urine, and serum. (author)

  5. Polymer optical fiber with Rhodamine doped cladding for fiber light systems

    Energy Technology Data Exchange (ETDEWEB)

    Narro-García, R., E-mail: roberto.narro@gmail.com [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Quintero-Torres, R. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Domínguez-Juárez, J.L. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Cátedras CONACyT, Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Ocampo, M.A. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico)

    2016-01-15

    Both preform and polymer optical fiber with a Poly(methyl methacrylate) core and THV–Rhodamine 6G cladding were characterized. UV–vis absorbance, photoluminescence spectra and lifetime of the preform were measured. Axial and lateral photoluminescence spectra of the polymer optical fiber were studied under 404 nm excitation in order to study the illumination performance of the fiber. It was observed that the peak wavelength from the fiber photoluminescence spectra is higher than the peak wavelength from the fiber preform and that the peak wavelength from the fiber photoluminescence spectra is red shifted with the fiber length in the case of axial emission. The obtained results suggest the influence of self-absorption on the photoluminescence shape. Strong lateral emission along the fiber was observed with the naked eyes in all the cases. The lateral photoluminescence spectra show that the lateral emission is a combination between the pump laser and the Rh6G molecule photoluminescence. The results suggest that this polymer optical fiber could be a potential candidate for the development of fiber lighting systems. - Highlights: • Axial and lateral emission along the fiber was studied. • Self-absorption effect was confirmed in the case of axial photoluminescence. • The lateral emission is a combination between the laser and the RhG6 emission. • This fiber could be a potential candidate for the development of lighting systems.

  6. Development of magnetic luminescent core/shell nanocomplex particles with fluorescence using Rhodamine 6G

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hee Uk; Song, Yoon Seok [Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701 (Korea, Republic of); Park, Chulhwan [Department of Chemical Engineering, Kwangwoon University, 447-1 Wolgye-Dong, Nowon-Gu, Seoul 139-701 (Korea, Republic of); Kim, Seung Wook, E-mail: kimsw@korea.ac.kr [Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701 (Korea, Republic of)

    2012-12-15

    Graphical abstract: Display Omitted Highlights: ► A simple method was developed to synthesize Co-B/SiO{sub 2}/dye/SiO{sub 2} composite particles. ► The magnetic particle shows that highly luminescent and core/shell particles are formed. ► Such core/shell particles can be easily suspended in water. ► The magnetic particles could detect fluorescence for the application of biosensor. -- Abstract: A simple and reproducible method was developed to synthesize a novel class of Co-B/SiO{sub 2}/dye/SiO{sub 2} composite core/shell particles. Using a single cobalt core, Rhodamine 6G of organic dye molecules was entrapped in a silica shell, resulting in core/shell particles of ∼200 nm diameter. Analyses using a variety of techniques such as transmission electron microscopy, X-ray photoelectron spectroscopy, vibration sample magnetometry, confocal laser scanning microscopy, and fluorescence intensity demonstrated that dye molecules were trapped inside the core/shell particles. A photoluminescence investigation showed that highly luminescent and photostable core/shell particles were formed. Such core/shell particles can be easily suspended in water. The synthesized magnetic particles could be used to detect fluorescence on glass substrate arrays for bioassay and biosensor applications.

  7. Highly selective rhodamine-based fluorescence turn-on chemosensor for Al3+ ion

    Science.gov (United States)

    Manjunath, Rangasamy; Kannan, Palaninathan

    2018-05-01

    A new rhodamine-based colorimetric and fluorescent turn-on chemosensor (L) has been designed and synthesized for selective and sensitive detection of Al3+ ion. The sensing behavior toward metal ion was investigated by UV/Vis and fluorescence spectroscopy. Upon addition of Al3+ ion to solution of L provided a visual color change as well as significantly fluorescent enhancement, while other metal ions including Na+, Mg2+, K+, Mn2+, Fe3+, Ni2+, Cu2+, Zn2+, Pb2+, Cd2+ and Hg2+ ions fails to generate a distinct color and spectral changes, the distinct color change and rapid switch-on fluorescence also provide naked eye detection for Al3+ ion. The mechanism involved equilibrium between non-fluorescent spirocyclic form and highly fluorescent ring open form process was utilized and 1:2 stoichiometry for L-Al3+ complex formed with an association constant of 1.42 × 103 M-1. Moreover, chemosensor L was applied for living cell imaging and confirmed that can be used as a fluorescent probe for monitoring Al3+ ion in living cells.

  8. Ratiometric Time-Gated Luminescence Probe for Nitric Oxide Based on an Apoferritin-Assembled Lanthanide Complex-Rhodamine Luminescence Resonance Energy Transfer System.

    Science.gov (United States)

    Tian, Lu; Dai, Zhichao; Liu, Xiangli; Song, Bo; Ye, Zhiqiang; Yuan, Jingli

    2015-11-03

    Using apoferritin (AFt) as a carrier, a novel ratiometric luminescence probe based on luminescence resonance energy transfer (LRET) between a Tb(3+) complex (PTTA-Tb(3+)) and a rhodamine derivative (Rh-NO), PTTA-Tb(3+)@AFt-Rh-NO, has been designed and prepared for the specific recognition and time-gated luminescence detection of nitric oxide (NO) in living samples. In this LRET probe, PTTA-Tb(3+) encapsulated in the core of AFt is the energy donor, and Rh-NO, a NO-responsive rhodamine derivative, bound on the surface of AFt is the energy acceptor. The probe only emits strong Tb(3+) luminescence because the emission of rhodamine is switched off in the absence of NO. Upon reaction with NO, accompanied by the turn-on of rhodamine emission, the LRET from Tb(3+) complex to rhodamine occurs, which results in the remarkable increase and decrease of the long-lived emissions of rhodamine and PTTA-Tb(3+), respectively. After the reaction, the intensity ratio of rhodamine emission to Tb(3+) emission, I565/I539, is ∼24.5-fold increased, and the dose-dependent enhancement of I565/I539 shows a good linearity in a wide concentration range of NO. This unique luminescence response allowed PTTA-Tb(3+)@AFt-Rh-NO to be conveniently used as a ratiometric probe for the time-gated luminescence detection of NO with I565/I539 as a signal. Taking advantages of high specificity and sensitivity of the probe as well as its good water-solubility, biocompatibility, and cell membrane permeability, PTTA-Tb(3+)@AFt-Rh-NO was successfully used for the luminescent imaging of NO in living cells and Daphnia magna. The results demonstrated the efficacy of the probe and highlighted it's advantages for the ratiometric time-gated luminescence bioimaging application.

  9. Kinetic and catalytic analysis of mesoporous metal oxides on the oxidation of Rhodamine B

    Science.gov (United States)

    Xaba, Morena S.; Noh, Ji-Hyang; Mokgadi, Keabetswe; Meijboom, Reinout

    2018-05-01

    In this study, we demonstrate the synthesis and catalytic activity of different mesoporous transition metal oxides, silica (SiO2), copper oxide (CuO), chromium oxide (Cr2O3), iron oxide (Fe2O3) cobalt oxide (Co3O4), cerium oxide (CeO2) and nickel oxide (NiO), on the oxidation of a pollutant dye, Rhodamine B (RhB). These metal oxides were synthesized by inverse micelle formation method and characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), adsorption-desorption isotherms (BET) and H2-temperature programmed reduction (TPR). UV-vis spectrophotometry was used to monitor the time-resolved absorbance of RhB at λmax = 554 nm. Mesoporous copper oxide was calcined at different final heating temperatures of 250, 350, 450 and 550 °C, and each mesoporous copper oxide catalyst showed unique physical properties and catalytic behavior. Mesoporous CuO-550 with the smallest characteristic path length δ, proved to be the catalyst of choice for the oxidation of RhB in aqueous media. We observed that the oxidation of RhB in aqueous media is dependent on the crystallite size and characteristic path length of the mesoporous metal oxide. The Langmuir-Hinshelwood model was used to fit the experimental data and to prove that the reaction occurs on the surface of the mesoporous CuO. The thermodynamic parameters, EA, ΔH#, ΔS# and ΔG# were calculated and catalyst recycling and reusability were demonstrated.

  10. Decolourization of Rhodamine B: A swirling jet-induced cavitation combined with NaOCl.

    Science.gov (United States)

    Mancuso, Giuseppe; Langone, Michela; Laezza, Marco; Andreottola, Gianni

    2016-09-01

    A hydrodynamic cavitation reactor (Ecowirl) based on swirling jet-induced cavitation has been used in order to allow the degradation of a waste dye aqueous solution (Rhodamine B, RhB). Cavitation generated by Ecowirl reactor was directly compared with cavitation generated by using multiple hole orifice plates. The effects of operating conditions and parameters such as pressure, pH of dye solution, initial concentration of RhB and geometry of the cavitating devices on the degradation rate of RhB were discussed. In similar operative conditions, higher extents of degradation (ED) were obtained using Ecowirl reactor rather than orifice plate. An increase in the ED from 8.6% to 14.7% was observed moving from hole orifice plates to Ecowirl reactor. Intensification in ED of RhB by using hydrodynamic cavitation in presence of NaOCl as additive has been studied. It was found that the decolourization was most efficient for the combination of hydrodynamic cavitation and chemical oxidation as compared to chemical oxidation and hydrodynamic cavitation alone. The value of ED of 83.4% was reached in 37min using Ecowirl combined with NaOCl (4.0mgL(-1)) as compared to the 100min needed by only mixing NaOCl at the same concentration. At last, the energetic consumptions of the cavitation devices have been evaluated. Increasing the ED and reducing the treatment time, Ecowirl reactor resulted to be more energy efficient as compared to hole orifice plates, Venturi and other swirling jet-induced cavitation devices, as reported in literature. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Heat response of mouse tumor cells treated with 5-thio-D-glucose and Rhodamine-123

    International Nuclear Information System (INIS)

    Rhee, J.G.; Lyons, J.C.; Song, C.W.

    1987-01-01

    Cellular heat-sensitivity has been known to depend on intracellular energy. The authors studied the thermal response of cultured SCK mammary carcinoma cells in vitro, following glycolytic inhibition with 5-thio-D-glucose (TG) and mitochondrial inactivation with Rhodamine-123 (Rh). The cells in exponential growth phase in RPMI 1640 medium supplemented with serum and antibiotics were exposed to medium containing Rh and/or TG, heated in a prewarmed water bath, and the clonogenic survivals of the heated cells were determined. Thermal cell killing by the 30 min. heating was increased, when 10 and 20 μg/ml Rh were present in the medium at temperatures above 42 0 and 40 0 C, respectively. The slope of the heat survival curve for 43 0 C heating became steeper in the presence of 10 and 20 μg/ml Rh, and the initial shoulder of the survival curve was unaltered at the dose of 10 μg/ml Rh, but disappeared at 20 μg/ml. A TG dose of 3 mg/ml, which is about 10 times that necessary to kill 90% of cells in 5 hrs. under hypoxic condition, was ineffective in altering any parameters of the heat survival curve of aerobic cells. The combined effect of TG and Rh on the thermal cell killing in aerobic condition did not exceed the effect of Rh alone. The above results indicate that the energy supply derived by mitochondria is an important determinant for the shape of heat survival curve of the proliferating and aerobic SCK tumor cells

  12. Mixed Hemi/Ad-Micelle Sodium Dodecyl Sulfate-Coated Magnetic Iron Oxide Nanoparticles for the Efficient Removal and Trace Determination of Rhodamine-B and Rhodamine-6G.

    Science.gov (United States)

    Ranjbari, Elias; Hadjmohammadi, Mohammad Reza; Kiekens, Filip; De Wael, Karolien

    2015-08-04

    Mixed hemi/ad-micelle sodium dodecyl sulfate (SDS)-coated magnetic iron oxide nanoparticles (MHAMS-MIONPs) were used as an efficient adsorbent for both removal and preconcentration of two important carcinogenic xanthine dyes named rhodamine-B (RB) and rhodamine-6G (RG). To gain insight in the configuration of SDS molecules on the surface of MIONPs, zeta potential measurements were performed in different [SDS]/[MIONP] ratios. Zeta potential data indicated that mixed hemi/ad-micelle MHAM was formed in [SDS]/[MIONP] ratios over the range of 1.1 to 7.3. Parameters affecting the adsorption of dyes were optimized as removal efficiency by one variable at-a-time and response surface methodology; the obtained removal efficiencies were ∼100%. Adsorption kinetic and equilibrium studies, under the optimum condition (pH = 2; amount of MIONPs = 87.15 mg; [SDS]/[MIONP] ratio = 2.9), showed that adsorption of both dyes are based on the pseudo-second-order and the Langmuir isotherm models, respectively. The maximum adsorption capacities for RB and RG were 385 and 323 mg g(-1), respectively. MHAMS-MIONPs were also applied for extraction of RB and RG. Under optimum conditions (pH = 2; amount of damped MHAMS-MIONPs = 90 mg; eluent solvent volume = 2.6 mL of 3% acetic acid in acetonitrile), extraction recoveries for 0.5 mg L(-1) of RB and RG were 98% and 99%, with preconcentration factors of 327 and 330, respectively. Limit of detection obtained for rhodamine dyes were <0.7 ng mL(-1). Finally, MHAMS-MIONPs were successfully applied for both removal and trace determination of RB and RG in environmental and wastewater samples.

  13. Highly selective coextraction of rhodamine B and dibenzyl phthalate based on high-density dual-template imprinted shells on silica microparticles.

    Science.gov (United States)

    Long, Zerong; Xu, Weiwei; Peng, Yumei; Lu, Yi; Luo, Qian; Qiu, Hongdeng

    2017-01-01

    A simple one-pot approach based on molecularly imprinted polymer shells dispersed on the surface of silica for simultaneous determination of rhodamine B and dibenzyl phthalate (DBzP) has been developed. Highly dense molecularly imprinted polymer shells were formed in the mixture of acetonitrile and toluene by the copolymerization of methacrylic acid and ethylene glycol dimethacrylate, as well as two templates, rhodamine B and dibenzyl phthalate, directed by the vinyl end groups functional monolayer at surface silica microspheres after 3-methacryloxypropyl trimethoxysilane modification. The obtained imprinted polymer shells showed large average pore diameter (102.5 nm) and about 100 nm shell thickness. The imprinted particles also showed high imprinting factor (α RhB = 3.52 and α DBzP = 3.94), rapid binding kinetics, and excellent selective affinity capacity for rhodamine B and dibenzyl phthalate containing another three competitors in mixed solution. Moreover, the imprinted particles coupled with ultra high performance liquid chromatography was successfully applied to simultaneous analysis of rhodamine B and dibenzyl phthalate in two spiked beverage samples with average recoveries in the range of 88.0-93.0% for rhodamine B and 84.0-92.0% for dibenzyl phthalate with the relative standard deviation lower than 5.1%. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe.

    Science.gov (United States)

    Soršak, Eva; Volmajer Valh, Julija; Korent Urek, Špela; Lobnik, Aleksandra

    2018-04-14

    This study presents chemical modification of a Rhodamine B (RhB) sensor probe by ethylenediamine (EDA), and investigation of its spectral as well as sensor properties to the various metals. The synthesised N -(Rhodamine-B)-lactam-ethylenediamine (RhB-EDA) fluorescent probe shows interesting optical sensor properties, and high sensitivity and selectivity to Ag⁺ ions among all the tested metal ions (K⁺, Mg 2+ , Cu 2+ , Ni 2+ , Fe 2+ , Pb 2+ , Na⁺, Mn 2+ , Li⁺, Al 3+ , Co 2+ , Hg 2+ , Sr 2+ , Ca 2+ , Ag⁺, Cd 2+ and Zn 2+ ), while the well-known Rhodamine B (RhB) fluorescent probe shows much less sensitivity to Ag⁺ ions, but high sensitivity to Fe 2+ ions. The novel fluorescent sensor probe RhB-EDA has the capabilities to sense Ag⁺ ions up to µM ranges by using the fluorescence quenching approach. The probe displayed a dynamic response to Ag⁺ in the range of 0.43 × 10 -3 -10 -6 M with a detection limit of 0.1 μM. The sensing system of an RhB-EDA novel fluorescent probe was optimised according to the spectral properties, effect of pH and buffer, photostability, incubation time, sensitivity, and selectivity. Since all the spectral and sensing properties were tested in green aqueous media, although many other similar sensor systems rely on organic solvent solutions, the RhB-EDA sensing probe may be a good candidate for measuring Ag⁺ ions in real-life applications.

  15. Effect of porphyrin on photocatalytic activity of TiO2 nanoparticles toward Rhodamine B photodegradation.

    Science.gov (United States)

    Ahmed, M A; Abou-Gamra, Z M; Medien, H A A; Hamza, M A

    2017-11-01

    As known, porphyrins have central role in photosynthesis, biological oxidation and reduction and oxygen transport beside to their intensive color which qualify them to be good photosensitizers. Herein, tetra (4-carboxyphenyl) porphyrin (TCPP) was prepared by a simple one-pot synthesis to use as a visible antenna for TiO 2 nanoparticles that were prepared via a simple template-free sol-gel method. Various loading percentages of TCPP (0.05-1%) were incorporated on the surface of TiO 2 as photosensitizer for photocatalytic degradation of Rhodamine B (Rh B) dye as a primary cationic pollutant model. Among them, 0.1% TCPP-TiO 2 was the most reactive sample. It was found that the photoactivity of 0.1% TCPP-TiO 2 sample (0.5g/L) was approximately 1.5 times greater than that of pure TiO 2 (0.5g/L) toward the degradation of Rh B (1×10 -5 M) under UV-A irradiation. Transient fluorescence decay measurements showed that the life time of TiO 2 excited state has doubled after anchoring TCPP, thus the probability of electron-hole recombination has decreased. The samples were characterized by XRD, HR-TEM, DRS and N 2 adsorption-desorption isotherms. The XRD patterns confirmed the successful preparation of TiO 2 nanoparticles with average crystalline size of 25.7nm. Also, XRD patterns suggested the presence of mixed phase TiO 2 nanoparticles of 77% anatase and 23% rutile. DRS showed that the characteristic peaks of TCPP covered the whole visible range 400-700nm. HR-TEM images showed the spheroids shape of TiO 2 nanoparticles and confirmed the presence of anatase and rutile phases as suggested from XRD data. The different parameters affecting the photodegradation of Rh B dye such as catalyst dose, dye concentration and pH were studied to obtain the optimum conditions. Almost complete degradation of Rh B was obtained which confirmed by HPLC and TOC measurements. The effect of scavengers was studied to indicate the most active species. TCPP-TiO 2 gave a good response toward the

  16. Enhanced visible light photocatalytic degradation of Rhodamine B over phosphorus doped graphitic carbon nitride

    Energy Technology Data Exchange (ETDEWEB)

    Chai, Bo, E-mail: willycb@163.com; Yan, Juntao; Wang, Chunlei; Ren, Zhandong; Zhu, Yuchan

    2017-01-01

    Highlights: • The phosphorus doped g-C{sub 3}N{sub 4} photocatalysts are synthesized by a co-pyrolysis procedure. • The crystal phase, morphology, and optical property of P doped g-C{sub 3}N{sub 4} are characterized. • The P doped g-C{sub 3}N{sub 4} photocatalysts show the improved photocatalytic activity. • The possible mechanism for enhanced photocatalytic activity is proposed. - Abstract: Phosphorus doped graphitic carbon nitride (g-C{sub 3}N{sub 4}) was easily synthesized using ammonium hexafluorophosphate (NH{sub 4}PF{sub 6}) as phosphorus source, and ammonium thiocyanate (NH{sub 4}SCN) as g-C{sub 3}N{sub 4} precursor, through a direct thermal co-polycondensation procedure. The obtained phosphorus doped g-C{sub 3}N{sub 4} was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FTIR), UV–vis diffuse reflectance absorption spectra (UV-DRS), photoelectrochemical measurement and photoluminescence spectra (PL). The photocatalytic activities of phosphorus doped g-C{sub 3}N{sub 4} samples were evaluated by degradation of Rhodamine B (RhB) solution under visible light irradiation. The results showed that the phosphorus doped g-C{sub 3}N{sub 4} had a superior photocatalytic activity than that of pristine g-C{sub 3}N{sub 4}, attributing to the phosphorus atoms substituting carbon atoms of g-C{sub 3}N{sub 4} frameworks to result in light harvesting enhancement and delocalized π-conjugated system of this copolymer, beneficial for the increase of photocatalytic performance. The photoelectrochemical measurements also verified that the charge carrier separation efficiency was promoted by phosphorus doping g-C{sub 3}N{sub 4}. Moreover, the tests of radical scavengers demonstrated that the holes (h{sup +}) and superoxide radicals (·O{sub 2}{sup −}) were the main active species for the

  17. One-pot synthesis and characterization of rhodamine derivative-loaded magnetic core-shell nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Jin, E-mail: jzhang@eng.uwo.ca; Li Jiaxin [University of Western Ontario, Department of Chemical and Biochemical Engineering (Canada); Razavi, Fereidoon S. [Brock University, Department of Physics (Canada); Mumin, Abdul Md. [University of Western Ontario, Department of Chemical and Biochemical Engineering (Canada)

    2011-05-15

    A new method to produce elaborate nanostructure with magnetic and fluorescent properties in one entity is reported in this article. Magnetite (Fe{sub 3}O{sub 4}) coated with fluorescent silica (SiO{sub 2}) shell was produced through the one-pot reaction, in which one reactor was utilized to realize the synthesis of superparamagnetic core of Fe{sub 3}O{sub 4}, the formation of SiO{sub 2} coating through the condensation and polymerization of tetraethylorthosilicate (TEOS), and the encapsulation of tetramethyl rhodamine isothiocyanate-dextran (TRITC-dextran) within silica shell. Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) analysis, and X-ray diffraction (XRD) were carried out to investigate the core-shell structure. The magnetic core of the core-shell nanoparticles is 60 {+-} 10 nm in diameter. The thickness of the fluorescent SiO{sub 2} shell is estimated at 15 {+-} 5 nm. In addition, the fluorescent signal of the SiO{sub 2} shell has been detected by the laser confocal scanning microscopy (LCSM) with emission wavelength ({lambda}{sub em}) at 566 nm. In addition, the magnetic properties of TRITC-dextran loaded silica-coating iron oxide nanoparticles (Fe{sub 3}O{sub 4}-SiO{sub 2} NPs) were studied. The hysteresis loop of the core-shell NPs measured at room temperature shows that the saturation magnetization (M{sub s}) is not reached even at the field of 70 kOe (7T). Meanwhile, the very low coercivity (H{sub c}) and remanent magnetization (M{sub r}) are 0.375 kOe and 6.6 emu/g, respectively, at room temperature. It indicates that the core-shell particles have the superparamagnetic properties. The measured blocking temperature (T{sub B}) of the TRITC-dextran loaded Fe{sub 3}O{sub 4}-SiO{sub 2} NPs is about 122.5 K. It is expected that the multifunctional core-shell nanoparticles can be used in bio-imaging.

  18. A contribution to the study of radioactive waste dilution in the Rhone involving tests with a rhodamine B tracer; Contribution a l'etude de la dilution des effluents radioactifs dans le Rhone par le rejet experimental de rhodamine B

    Energy Technology Data Exchange (ETDEWEB)

    Rodier, J.; Marichal, M. [Commissariat a l' energie atomique et aux energies alternatives - CEA, Centre de production de plutonium de Marcoule, Service de protection contre les radiations (France)

    1961-07-01

    The process whereby waste from the Marcoule plant mixes with the water in the Rhone was followed in tests with rhodamine as a chemical tracer. Satisfactory dispersion was noted less than 4 km downstream from the waste discharge duct outlet, and the degree of homogeneity was considered to be satisfactory at the bridge of Roquemaure, und perfect at Avignon. This investigation not only revealed a complete absence of any preferential flow paths containing high radioactive waste concentrations, but it also enabled the most representative points to be selected at which to take Rhone water samples during future radioactive waste discharges. Reprint of a paper published in 'La Houille Blanche' N. 5 - Aug 196, p. 636-641 [French] L'emploi de la rhodamine comme traceur chimique a permis de suivre l'evolution du melange des effluents du Centre de Marcoule aux eaux du Rhone. La dispersion est deja satisfaisante a moins de 4 km en aval de la conduite des rejets, et l'homogeneite peut etre consideree comme atteinte au pont de Roquemaure et parfaite a Avignon. Cette etude a montre que les veines preferentielles ou se concentrait l'ecoulement des effluents radioactifs n'existent pas. Elle a permis de preciser en outre les emplacements les plus representatifs des points d'echantillonnage des eaux du Rhone au cours des rejets. Reproduction d'un article publie dans 'La houille blanche' N. 5 - Aug 196, p. 636-641.

  19. Development of a rapid, simple and sensitive HPLC-FLD method for determination of rhodamine B in chili-containing products.

    Science.gov (United States)

    Qi, Ping; Lin, Zhihao; Li, Jiaxu; Wang, ChengLong; Meng, WeiWei; Hong, Hong; Zhang, Xuewu

    2014-12-01

    In this work, a simple, rapid and sensitive analytical method for the determination of rhodamine B in chili-containing foodstuffs is described. The dye is extracted from samples with methanol and analysed without further cleanup procedure by high-performance liquid chromatography (HPLC) coupled to fluorescence detection (FLD). The influence of matrix fluorescent compounds (capsaicin and dihydrocapsaicin) on the analysis was overcome by the optimisation of mobile-phase composition. The limit of determination (LOD) and limit of quantification (LOQ) were 3.7 and 10 μg/kg, respectively. Validation data show a good repeatability and within-lab reproducibility with relative standard deviations rhodamine B in foodstuffs. This method is suitable for the routine analysis of rhodamine B due to its sensitivity, simplicity, reasonable time and cost. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Application of Taguchi L16 design method for comparative study of ability of 3A zeolite in removal of Rhodamine B and Malachite green from environmental water samples

    Science.gov (United States)

    Rahmani, Mashaallah; Kaykhaii, Massoud; Sasani, Mojtaba

    2018-01-01

    This study aimed to investigate the efficiency of 3A zeolite as a novel adsorbent for removal of Rhodamine B and Malachite green dyes from water samples. To increase the removal efficiency, effecting parameters on adsorption process were investigated and optimized by adopting Taguchi design of experiments approach. The percentage contribution of each parameter on the removal of Rhodamine B and Malachite green dyes determined using ANOVA and showed that the most effective parameters in removal of RhB and MG by 3A zeolite are initial concentration of dye and pH, respectively. Under optimized condition, the amount predicted by Taguchi design method and the value obtained experimentally, showed good closeness (more than 94.86%). Good adsorption efficiency obtained for proposed methods indicates that, the 3A zeolite is capable to remove the significant amounts of Rhodamine B and Malachite green from environmental water samples.

  1. Synthesis of Tb{sub 4}O{sub 7} complexed with reduced graphene oxide for Rhodamine-B absorption

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Hui, E-mail: hope@lzu.edu.cn [School of Physical Science and Technology, Key Laboratory for Magnetism and Magnetic Materials of Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Zhou, Yang; Chen, Keqin [School of Physical Science and Technology, Key Laboratory for Magnetism and Magnetic Materials of Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Li, Xiaolong, E-mail: lixiaolong@sinap.ac.cn [Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204 (China)

    2016-05-15

    Highlights: • Tb–rGO composite was fabricated via a facile thermally reduction process. • The green and blue emissions were both observed in the composite. • The composite exhibited efficient absorption capability for Rhodamine-B. - Abstract: Tb{sub 4}O{sub 7} complexed with reduced graphene oxide composite (Tb–rGO) had been designed and fabricated by a facile thermal reduction method. The formation of Tb{sub 4}O{sub 7} particles and reduction of graphene oxide (GO) occurred simultaneously, and partial terbium ions would be complexed with rGO via oxygen-containing function groups on rGO sheets. Introducing of terbium ions could effectively tune the photoluminescence properties of rGO, and the composite exhibited the typical green emission of terbium ions as well as the blue self-luminescence of graphene entered at 440 nm. Moreover, Tb–rGO had demonstrated its high capability as an organic dye (Rhodamine-B) scavenger with high speed and efficiency. The findings showed the promising applications for large-scale removal of organic dye contaminants, especially in the field of waste water treatment.

  2. Rhodamine-based chemodosimeter for fluorescent determination of Hg(2+) in 100% aqueous solution and in living cells.

    Science.gov (United States)

    Li, Dan; Li, Chun-Yan; Li, Yong-Fei; Li, Zhi; Xu, Fen

    2016-08-31

    A rhodamine spirolactam derivative (1) bearing a hydrophilic carboxylic acid group is developed as a fluorescent chemodosimeter for bivalent mercury ions (Hg(2+)) in 100% aqueous solution. It exhibits a highly sensitive "turn-on" fluorescent response toward Hg(2+) with a 42-fold fluorescence intensity enhancement under 1 equiv. of Hg(2+) added. The chemodosimeter can be applied to the quantification of Hg(2+) with a linear range covering from 3.0 × 10(-7) to 1.0 × 10(-5) M and a detection limit of 9.7 × 10(-8) M. Most importantly, the fluorescence changes of the chemodosimeter are remarkably specific for Hg(2+) in the presence of other metal ions, which meet the selective requirements for practical application. Moreover, the experiment results show that the response behavior of 1 towards Hg(2+) is pH independent in neutral condition (pH 5.0-8.0) and the response is fast (response time less than 3 min). Furthermore, the ring-opening mechanism of the rhodamine spirolactam induced by Hg(2+) was supported by NMR, MS, and DFT theoretical calculations. In addition, the proposed chemodosimeter has been used to detect Hg(2+) in water samples and image Hg(2+) in living cells with satisfying results. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. A novel fluorescent probe based on rhodamine hydrazone derivatives bearing a thiophene group for Al³⁺.

    Science.gov (United States)

    Li, Meng-xiao; Zhang, Xia; Fan, Yu-hua; Bi, Cai-feng

    2016-05-01

    In the present work, a novel 5-methyl-thiophene-carbaldehyde-functionalized rhodamine 6G Schiff base (RA) was designed and easily prepared as an Al(3+) fluorescent and colorimetric probe, which could selectively and sensitively detect Al(3+) by showing enhanced fluorescence emission. Meanwhile distinct color variation from colorless to pink also provided 'naked eye' detection of Al(3+), due to the ring spirolactam opening of the rhodamine derivative. Other metal ions (including K(+), Mg(2+), Na(+), Ba(2+), Mn(2+), Cd(2+), Fe(2+), Ni(2+), Pb(2+), Zn(2+), Hg(2+), Co(2+), Li(+), Sr(2+) and Cu(2+)) could only induce limited interference. The detection limit of the fluorescent probe was estimated to be 4.17 × 10(-6) M, the binding constant of the RA-Al(3+) complex was 1.4 × 10(6)  M(-1). Moreover, this fluorescent probe RA possessed high reversibility. As aluminum is a ubiquitous metal in nature and plays vital roles in many biological processes, this chemosensor could be explored for biological study applications. Copyright © 2015 John Wiley & Sons, Ltd.

  4. A solvent-dependent fluorescent detection method for Fe(3+) and Hg(2+) based on a rhodamine B derivative.

    Science.gov (United States)

    Li, Xutian; Yin, Yue; Deng, Junjie; Zhong, Huixian; Tang, Jian; Chen, Zhi; Yang, Liting; Ma, Li-Jun

    2016-07-01

    A new rhodamine B-benzofurazan based fluorescent probe (1) for Fe(3+) and Hg(2+) was synthesized. In aqueous solution containing 30% (v/v) ethanol, probe 1 shows a high selective fluorescent enhancement recognition to Fe(3+) with a binding ratio of 1:1 (probe 1: Fe(3+)), when the concentration of Fe(3+) is less than that of the probe. When the concentration of Fe(3+) is higher than that of the probe, it shows fluorescent "turn-on" response to Fe(3+) by opening the rhodamine spirolactam with a binding ratio of 1:2 (probe 1: Fe(3+)). Furthermore, probe 1 displays a high selectivity and a hypersensitivity (detection limit is 4.4nM) to Hg(2+) with a binding ratio of 1:1 in ethanol. NMR and UV-vis experiments indicate that the different fluorescent recognition signals to Fe(3+) and Hg(2+) are derived from different binding modes of 1-Fe(3+) and 1-Hg(2+). Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Spectrophotometric determination of trace carbaryl in water and grain samples by inhibition of the rhodamine-B oxidation.

    Science.gov (United States)

    Gupta, Nirja; Pillai, Ajai Kumar; Parmar, Prachi

    2015-03-15

    A novel, sensitive, selective and simple kinetic spectrophotometric method has been developed for determination of trace levels of carbaryl based on its inhibitory effect on the oxidation of rhodamine-B by chlorine and bromine released from reaction of potassium bromate with hydrochloric acid in micellar medium. A linear relationship was observed between the inhibitory effect and the concentration of the compound. The absorbance was monitored at the maximum wavelength of 555 nm. The effect of different parameters such as pH, temperature and concentration of rhodamine-B, potassium bromate and surfactant on the reaction were investigated and optimum conditions were established. Under the selected experimental conditions, carbaryl was determined in the range of 0.04-0.4 μg mL(-1). Sandell's sensitivity and molar absorptivity were found to be 0.00055 μg cm(-2) and 3.658×10(5) L mol(-1) cm(-1) respectively. The proposed method was applied satisfactorily for the determination of carbaryl in water and different grain samples. The results were compared with those obtained by reference method and were found to be in agreement. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Determination of fluvoxamine maleate in human urine and human serum using alkaline KMnO4 -rhodamine B chemiluminescence.

    Science.gov (United States)

    Yang, Dongqin; He, Yanyan; Chen, Funan

    2017-09-01

    The flow-injection chemiluminescence (FI-CL) behavior of a gold nanocluster (Au NC)-enhanced rhodamine B-KMnO 4 system was studied under alkaline conditions for the first time. In the present study, the as-prepared bovine serum albumin-stabilized Au NCs showed excellent stability and reproducibility. The addition of trace levels of fluvoxamine maleate (Flu) led to an obvious decline in CL intensity in the rhodamine B-KMnO 4 -Au NCs system, which could be used for quantitative detection of Flu. Under optimized conditions, the proposed CL system exhibited a favorable analytical performance for Flu determination in the range 2 to 100 μg ml -1 . The detection limit for Flu measurement was 0.021 μg ml -1 . Moreover, this newly developed system revealed outstanding selectivity for Flu detection when compared with a multitude of other species, such as the usual ions, uric acid and a section of hydroxy compounds. Additionally, CL spectra, UV-visible spectroscopes and fluorescence spectra were measured in order to determine the possible reaction mechanism. This approach could be used to detect Flu in human urine and human serum samples with the desired recoveries and could have promising application under physiological conditions. Copyright © 2017 John Wiley & Sons, Ltd.

  7. Study on adsorption of rhodamine B onto Beta zeolites by tuning SiO2/Al2O3 ratio.

    Science.gov (United States)

    Cheng, Zhi-Lin; Li, Yan-Xiang; Liu, Zan

    2018-02-01

    The exploration of the relationship between zeolite composition and adsorption performance favored to facilitate its better application in removal of the hazardous substances from water. The adsorption capacity of rhodamine B (RB) onto Beta zeolite from aqueous solution was reported. The relationship between SiO 2 /Al 2 O 3 ratio and adsorption capacity of Beta zeolite for RB was explored. The structure and physical properties of Beta zeolites with various SiO 2 /Al 2 O 3 ratios were determined by XRD, FTIR, TEM, BET, UV-vis and so on characterizations. The adsorption behavior of rhodamine B onto Beta zeolite matched to Langmuir adsorption isotherm and more suitable description for the adsorption kinetics was a pseudo-second-order reaction model. The maximum adsorption capacity of the as-prepared Beta zeolite with SiO 2 /Al 2 O 3 = 18.4 was up to 27.97mg/g. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. A study on linear and non-linear optical constants of Rhodamine B thin film deposited on FTO glass

    Science.gov (United States)

    Yahia, I. S.; Jilani, Asim; Abutalib, M. M.; AlFaify, S.; Shkir, M.; Abdel-wahab, M. Sh.; Al-Ghamdi, Attieh A.; El-Naggar, A. M.

    2016-06-01

    The aim of this research was to fabricate/deposit the good quality thin film of Rhodamine B dye on fluorine doped tin oxide glass substrate by the low cost spin coating technique and study their linear and nonlinear optical parameters. The thickness of the thin film was measured about 300 nm with alpha step system. The transmittance of the fabricated thin film was found to be above 75% corresponding to the fluorine doped tin oxide layer. The structural analysis was performed with X-rays diffraction spectroscopy. Atomic force microscope showed the topographic image of deposited thin film. Linear optical constant like absorption coefficient, band gap, and extinction index was calculated. The dielectric constant was calculated to know the optical response of Rhodamine B dye over fluorine doped tin oxide substrate. The nonlinear optical constant like linear optical susceptibility χ(1), nonlinear optical susceptibility χ(3), nonlinear refractive index (n2) were calculated by spectroscopic method. This method has advantage over the experimental method like Z-Scan for organic dye base semiconductors for future advance optoelectronics applications like dye synthesis solar cell.

  9. A novel three-input monomolecular logic circuit on a rhodamine inspired bio-compatible bi-compartmental molecular platform

    International Nuclear Information System (INIS)

    Mistri, Tarun; Bhowmick, Rahul; Katarkar, Atul; Chaudhuri, Keya; Ali, Mahammad

    2017-01-01

    Methodological synthesis of a new biocompatible bi-compartmental rhodamine based probe (L 3 ) provides a multi-inputs and multi-outputs molecular logic circuit based on simple chemosensing phenomena. Spectroscopic responses of Cu 2+ and Hg 2+ towards L 3 together with reversible binding of S 2- with L 3 -Cu 2+ and L 3 -Hg 2+ complexes help us to construct a thee-input molecular circuit on their control and sequential addition to a solution of L 3 in a mixed organo-aqueous medium. We have further successfully encoded binary digits out of these inputs and outputs which may convert a three-digit input string into a two-digit output string resulting a simple monomolecular logic circuit. Such a molecular ‘Boolean’ logic operation may improve the complexity of logic gate circuitry and computational speed and may be useful to employ in potential biocompatible molecular logic platforms. - Graphical abstract: A new bi-compartmental molecular system equipped with Rhodamine fluorophore unit provides a Multi-inputs and Multi-outputs Molecular Logic Circuit based on a very simple observation of chemosensing activities.

  10. A novel three-input monomolecular logic circuit on a rhodamine inspired bio-compatible bi-compartmental molecular platform

    Energy Technology Data Exchange (ETDEWEB)

    Mistri, Tarun; Bhowmick, Rahul [Department of Chemistry, Jadavpur University, 188 Raja S.C. Mullick Road, Kolkata 700032 (India); Katarkar, Atul; Chaudhuri, Keya [Molecular & Human Genetics Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata 700032 (India); Ali, Mahammad, E-mail: mali@chemistry.jdvu.ac.in [Department of Chemistry, Jadavpur University, 188 Raja S.C. Mullick Road, Kolkata 700032 (India)

    2017-05-15

    Methodological synthesis of a new biocompatible bi-compartmental rhodamine based probe (L{sup 3}) provides a multi-inputs and multi-outputs molecular logic circuit based on simple chemosensing phenomena. Spectroscopic responses of Cu{sup 2+} and Hg{sup 2+} towards L{sup 3} together with reversible binding of S{sup 2-} with L{sup 3}-Cu{sup 2+} and L{sup 3}-Hg{sup 2+} complexes help us to construct a thee-input molecular circuit on their control and sequential addition to a solution of L{sup 3} in a mixed organo-aqueous medium. We have further successfully encoded binary digits out of these inputs and outputs which may convert a three-digit input string into a two-digit output string resulting a simple monomolecular logic circuit. Such a molecular ‘Boolean’ logic operation may improve the complexity of logic gate circuitry and computational speed and may be useful to employ in potential biocompatible molecular logic platforms. - Graphical abstract: A new bi-compartmental molecular system equipped with Rhodamine fluorophore unit provides a Multi-inputs and Multi-outputs Molecular Logic Circuit based on a very simple observation of chemosensing activities.

  11. Delivery of cyclodextrin polymers to bacterial biofilms - An exploratory study using rhodamine labelled cyclodextrins and multiphoton microscopy.

    Science.gov (United States)

    Thomsen, Hanna; Benkovics, Gábor; Fenyvesi, Éva; Farewell, Anne; Malanga, Milo; Ericson, Marica B

    2017-10-15

    Cyclodextrin (CD) polymers are interesting nanoparticulate systems for pharmaceutical delivery; however, knowledge regarding their applications towards delivery into complex microbial biofilm structures is so far limited. The challenge is to demonstrate penetration and transport through the biofilm and its exopolysaccharide matrix. The ideal functionalization for penetration into mature biofilms is unexplored. In this paper, we present a novel set of rhodamine labelled βCD-polymers, with different charge moieties, i.e., neutral, anionic, and cationic, and explore their potential delivery into mature Staphylococcus epidermidis biofilms using multiphoton laser scanning microscopy (MPM). The S. epidermidis biofilms, being a medically relevant model organism, were stained with SYTO9. By using MPM, three-dimensional imaging and spectral investigation of the distribution of the βCD-polymers could be obtained. It was found that the cationic βCD-polymers showed significantly higher integration into the biofilms, compared to neutral and anionic functionalized βCDs. None of the carriers presented any inherent toxicity to the biofilms, meaning that the addition of rhodamine moiety does not affect the inertness of the delivery system. Taken together, this study demonstrates a novel approach by which delivery of fluorescently labelled CD nanoparticles to bacterial biofilms can be explored using MPM. Future studies should be undertaken investigating the potential in using cationic functionalization of CD based delivery systems for targeting anti-microbial effects in biofilms. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  12. A study on linear and non-linear optical constants of Rhodamine B thin film deposited on FTO glass

    Energy Technology Data Exchange (ETDEWEB)

    Yahia, I.S. [Nano-Science & Semiconductor Labs, Physics Department, Faculty of Education, Ain Shams University, Roxy, Cairo (Egypt); Department of Physics, Faculty of Science, King Khalid University, P.O. Box 9004, Abha (Saudi Arabia); Jilani, Asim, E-mail: asim.jilane@gmail.com [Centre of Nanotechnology, Physics Department-Faculty of Science-AL Faisaliah Campus, King Abdulaziz University, P.O. Box 80200, Jeddah 21589 (Saudi Arabia); Abutalib, M.M. [Centre of Nanotechnology, Physics Department-Faculty of Science-AL Faisaliah Campus, King Abdulaziz University, P.O. Box 80200, Jeddah 21589 (Saudi Arabia); AlFaify, S. [Nano-Science & Semiconductor Labs, Physics Department, Faculty of Education, Ain Shams University, Roxy, Cairo (Egypt); Shkir, M. [Department of Physics, Faculty of Science, King Khalid University, P.O. Box 9004, Abha (Saudi Arabia); Abdel-wahab, M.Sh.; Al-Ghamdi, Attieh A. [Centre of Nanotechnology, Physics Department-Faculty of Science-AL Faisaliah Campus, King Abdulaziz University, P.O. Box 80200, Jeddah 21589 (Saudi Arabia); El-Naggar, A.M. [Exploitation of Renewable Energy Applications in Saudi Arabia, Physics & Astronomy Department, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451 (Saudi Arabia)

    2016-06-01

    The aim of this research was to fabricate/deposit the good quality thin film of Rhodamine B dye on fluorine doped tin oxide glass substrate by the low cost spin coating technique and study their linear and nonlinear optical parameters. The thickness of the thin film was measured about 300 nm with alpha step system. The transmittance of the fabricated thin film was found to be above 75% corresponding to the fluorine doped tin oxide layer. The structural analysis was performed with X-rays diffraction spectroscopy. Atomic force microscope showed the topographic image of deposited thin film. Linear optical constant like absorption coefficient, band gap, and extinction index was calculated. The dielectric constant was calculated to know the optical response of Rhodamine B dye over fluorine doped tin oxide substrate. The nonlinear optical constant like linear optical susceptibility χ{sup (1)}, nonlinear optical susceptibility χ{sup (3)}, nonlinear refractive index (n{sub 2}) were calculated by spectroscopic method. This method has advantage over the experimental method like Z-Scan for organic dye base semiconductors for future advance optoelectronics applications like dye synthesis solar cell.

  13. Adsorption and transport of cadmium and rhodamine WT in pumice sand columns

    International Nuclear Information System (INIS)

    Pang, L.; Close, M.; Greenfield, H.; Stanton, G.

    2004-01-01

    The transport and attenuation of cadmium (Cd) and rhodamine WT (RWT) in a pumice sand aquifer media was investigated using column experiments to study a scenario of point-source contamination. A pore-water velocity of 1.7-1.8 m/day, which is a typical field groundwater velocity in a pumice sand aquifer system, was applied to triplicate columns. A pulse of a solution containing Cd and RWT, together with the conservative tracer tritiated water ( 3 H 2 O) at pH = 7, was introduced into the columns. Experimental results showed that concentration breakthrough curves (BTCs) of 3 H 2 O were symmetrical and fitted well into an equilibrium model. In contrast, BTCs of Cd and RWT were asymmetrical with significant tailings and fitted well with a two-site adsorption/desorption model. The symmetric 3 H 2 O BTCs suggest that physical non-equilibrium was absent in the experimental system, therefore the asymmetrical BTCs of Cd and RWT were attributed to chemical non-equilibrium. Modelling results showed that, in comparison with 3 H 2 O, Cd was apparently retarded by 101-108 times in pumice sand aquifer media (apparent adsorption coefficient 7.33-9.24 ml/g) and underwent a mass loss of 20-30% that was probably because of precipitation of CdCO 3 . As CdCO 3 is extremely insoluble, Cd precipitation would be irreversible and therefore it would not contribute to the tailing of the Cd BTCs. The experimental results suggest that the adsorption and desorption of Cd in pumice sand aquifer media in hydrodynamic conditions was a kinetic process. Cd desorption rates were two orders-of-magnitude slower than its adsorption rates. This resulted in a prolonged mean residence time for Cd in pumice sand aquifer media, which was 10-12 days in the 18-cm-long columns under a flow velocity of 1.7-1.8 m/day. Since the mean residence time is only indicative for the arrival of the central of mass in a contaminant BTC, the time required for the total disappearance of Cd will be much longer than the mean

  14. Polar red-emitting rhodamine dyes with reactive groups: synthesis, photophysical properties, and two-color STED nanoscopy applications.

    Science.gov (United States)

    Kolmakov, Kirill; Wurm, Christian A; Meineke, Dirk N H; Göttfert, Fabian; Boyarskiy, Vadim P; Belov, Vladimir N; Hell, Stefan W

    2014-01-03

    The synthesis, reactivity, and photophysical properties of new rhodamines with intense red fluorescence, two polar residues (hydroxyls, primary phosphates, or sulfonic acid groups), and improved hydrolytic stability of the amino-reactive sites (NHS esters or mixed N-succinimidyl carbonates) are reported. All fluorophores contain an N-alkyl-1,2-dihydro-2,2,4-trimethylquinoline fragment, and most of them bear a fully substituted tetrafluoro phenyl ring with a secondary carboxamide group. The absorption and emission maxima in water are in the range of 635-639 and 655-659 nm, respectively. A vastly simplified approach to red-emitting rhodamines with two phosphate groups that are compatible with diverse functional linkers was developed. As an example, a phosphorylated dye with an azide residue was prepared and was used in a click reaction with a strained alkyne bearing an N-hydroxysuccinimid (NHS) ester group. This method bypasses the undesired activation of phosphate groups, and gives an amphiphilic amino-reactive dye, the solubility and distribution of which between aqueous and organic phases can be controlled by varying the pH. The presence of two hydroxyl groups and a phenyl ring with two carboxyl residues in the dyes with another substitution pattern is sufficient for providing the hydrophilic properties. Selective formation of a mono-N-hydroxysuccinimidyl ester from 5-carboxy isomer of this rhodamine is reported. The fluorescence quantum yields varied from 58 to 92% for free fluorophores, and amounted to 18-64% for antibody conjugates in aqueous buffers. The brightness and photostability of these fluorophores facilitated two-color stimulated emission depletion (STED) fluorescence nanoscopy of biological samples with high contrast and minimal background. Selecting a pair of fluorophores with absorption/emission bands at 579/609 and 635/655 nm enabled two-color channels with low cross-talk and negligible background at approximately 40 nm resolution. Copyright

  15. Rhodamine-labeled 2beta-carbomethoxy-3beta-(3,4-dichlorophenyl)tropane analogues as high-affinity fluorescent probes for the dopamine transporter

    DEFF Research Database (Denmark)

    Cha, Joo Hwan; Zou, Mu-Fa; Adkins, Erika M

    2005-01-01

    linker. The resulting 2-substituted (5) and N-substituted (9) rhodamine-labeled ligands provided the highest DAT binding affinities expressed in COS-7 cells (Ki= 27 and 18 nM, respectively) in the series. Visualization of the DAT with 5 and 9 was demonstrated by confocal fluorescence laser scanning...

  16. Synthesis and characterization of core-shell magnetic molecularly imprinted polymers for solid-phase extraction and determination of Rhodamine B in food.

    Science.gov (United States)

    Su, Xiaomeng; Li, Xiaoyan; Li, Junjie; Liu, Min; Lei, Fuhou; Tan, Xuecai; Li, Pengfei; Luo, Weiqiang

    2015-03-15

    Core-shell magnetic molecularly imprinted polymers (MIPs) nanoparticles (NPs), in which a Rhodamine B-imprinted layer was coated on Fe3O4 NPs. were synthesized. First, Fe3O4 NPs were prepared by a coprecipitation method. Then, amino-modified Fe3O4 NPs (Fe3O4@SiO2-NH2) was prepared. Finally, the MIPs were coated on the Fe3O4@SiO2-NH2 surface by the copolymerization with functional monomer, acrylamide, using a cross-linking agent, ethylene glycol dimethacrylate; an initiator, azobisisobutyronitrile and a template molecule, Rhodamine B. The Fe3O4@MIPs were characterized using a scanning electron microscope, Fourier transform infrared spectrometer, vibrating sample magnetometer, and re-binding experiments. The Fe3O4@MIPs showed a fast adsorption equilibrium, a highly improved imprinting capacity, and significant selectivity; they could be used as a solid-phase extraction material and detect illegal addition Rhodamine B in food. A method was developed for the selective isolation and enrichment of Rhodamine B in food samples with recoveries in the range 78.47-101.6% and the relative standard deviation was <2%. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Synthesis of magnetic CoFe2O4/ordered mesoporous carbon nanocomposites and application in Fenton-like oxidation of rhodamine B.

    Science.gov (United States)

    Deng, Jing; Chen, Yi-Jing; Lu, Yu-An; Ma, Xiao-Yan; Feng, Shan-Fang; Gao, Naiyun; Li, Jun

    2017-06-01

    CoFe 2 O 4 /ordered mesoporous carbon (OMC) nanocomposites were synthesized and tested as heterogeneous peroxymonosulfate (PMS) activator for the removal of rhodamine B. Characterization confirmed that CoFe 2 O 4 nanoparticles were tightly bonded to OMC, and the hybrid catalyst possessed high surface area, pore volume, and superparamagnetism. Oxidation experiments demonstrated that CoFe 2 O 4 /OMC nanocomposites displayed favorable catalytic activity in PMS solution and rhodamine B degradation could be well described by pseudo-first-order kinetic model. Sulfate radicals (SO 4 - ·) were verified as the primary reactive species which was responsible for the decomposition of rhodamine B. The optimum loading ratio of CoFe 2 O 4 and OMC was determined to be 5:1. Under optimum operational condition (catalyst dosage 0.05 g/L, PMS concentration 1.5 mM, pH 7.0, and 25 °C), CoFe 2 O 4 /OMC-activated peroxymonosulfate system could achieve almost complete decolorization of 100 mg/L rhodamine B within 60 min. The enhanced catalytic activity of CoFe 2 O 4 /OMC nanocomposites compared to that of CoFe 2 O 4 nanoparticles could be attributable to the increased adsorption capacity and accelerated redox cycles between Co(III)/Co(II) and Fe(III)/Fe(II).

  18. Dual optoelectronic visual detection and quantification of spectroscopically silent heavy metal toxins: A multi-measurand sensing strategy based on Rhodamine 6G as chromo or fluoro ionophore

    Energy Technology Data Exchange (ETDEWEB)

    Prathish, K.P.; James, D.; Jaisy, J. [Inorganic and Polymeric Materials Group, National Institute for Interdisciplinary Science and Technology (CSIR), Industrial Estate (P.O), Pappanamcode, Trivandrum 695019 (India); Prasada Rao, T., E-mail: rao@csrrltrd.ren.nic.in [Inorganic and Polymeric Materials Group, National Institute for Interdisciplinary Science and Technology (CSIR), Industrial Estate (P.O), Pappanamcode, Trivandrum 695019 (India)

    2009-08-04

    A novel colorimetric chemo-sensor for the simultaneous visual detection and quantification of spectroscopically silent heavy metal toxins viz. cadmium, lead and mercury has been developed. This is based on the proposed sequential ligand exchange (SLE) mechanism of iodide from Pb-I{sup -}-Rhodamine 6G ion associate with citrate (without affecting ion associates of Cd and Hg) and subsequently from Cd-I{sup -}-Rhodamine 6G ion associate with EDTA (without affecting Hg-I{sup -}-Rhodamine 6G). Multi-measurand detection and quantification by colorimetry is possible as the individual toxins gives identical bathochromic shifts in aqueous solution, i.e. from 530 to 575 nm on formation of ternary ion associates in singular, binary and ternary mixtures. The visual detection provides a simple, quick and sensitive detection method in addition to quantification via spectrophotometry with Sandell sensitivities of 1.1, 15 and 2.5 {mu}g dm{sup -2} for cadmium, lead and mercury, respectively. The developed procedure has been successfully tested for the analysis of environmental (cast alkali, lead acid battery and zinc manufacturing industry effluents) samples. Furthermore, the multi-measurand quantification of the above-mentioned heavy metal toxins based on fluorescence quenching and use of Pyronine G as chromo-ionophore instead of Rhodamine 6G is also described.

  19. Simple and fast fluorescence detection of benzoyl peroxide in wheat flour by N-methoxy rhodamine-6G spirolactam based on consecutive chemical reactions

    International Nuclear Information System (INIS)

    Chen Wei; Shi Wen; Li Zhao; Ma Huimin; Liu Yang; Zhang Jinghua; Liu Qingjun

    2011-01-01

    Graphical abstract: A simple and fast method for fluorescence detection of benzoyl peroxide in wheat flour by N-methoxy rhodamine-6G spirolactam (1) is proposed based on consecutive chemical reactions. Highlights: ► Benzoyl peroxide can oxidize Fe 2+ into Fe 3+ . ► Fe 3+ selectively induces the opening of rhodamine spirolactam ring. ► The two reactions led to the development of a new fluorescent method for benzoyl peroxide. ► The method is simple and fast, and is used to detect benzoyl peroxide in wheat flour. - Abstract: Benzoyl peroxide (BPO) as a brightener is often added to wheat flour, and excessive use of this food additive is receiving increasing concern. Herein, a simple and fast method for fluorescence detection of BPO is proposed based on consecutive chemical reactions. In this approach, BPO first oxidizes Fe 2+ into Fe 3+ and the resulting Fe 3+ then induces the opening of the spirolactam ring of a new rhodamine derivative, N-methoxy rhodamine-6G spirolactam, switching on fluorescence of the detection system. More importantly, the fluorescence response of the reaction system to BPO is rather rapid and sensitive, with a detection limit of 6 mg kg −1 (k = 3), which makes it to be of great potential use in food safety analysis. The applicability of the proposed method has been successfully demonstrated on the determination of BPO in wheat flour samples.

  20. Dynamic adsorption of mixtures of Rhodamine B, Pb (II), Cu (II) and Zn(II) ions on composites chitosan-silica-polyethylene glycol membrane

    Science.gov (United States)

    Mahatmanti, F. W.; Rengga, W. D. P.; Kusumastuti, E.; Nuryono

    2018-04-01

    The adsorption of a solution mixture of Rhodamine B, Pb (II), Cu (II) and Zn(II) was studied using dynamic methods employing chitosan-silica-polyethylene glycol (Ch/Si/P) composite membrane as an adsorptive membrane. The composite Ch/Si/P membrane was prepared by mixing a chitosan-based membrane with silica isolated from rice husk ash (ASP) and polyethylene glycol (PEG) as a plasticizer. The resultant composite membrane was a stronger and more flexible membrane than the original chitosan-based membrane as indicated by the maximum percentage of elongation (20.5 %) and minimum Young’s Modulus (80.5 MPa). The composite membrane also showed increased mechanical and hydrophilic properties compared to the chitosan membranes. The membrane was used as adsorption membrane for Pb (II), Cu (II), Cd (II) ions and Rhodamine B dyes in a dynamic system where the permeation and selectivity were determined. The permeation of the components was observed to be in the following order: Rhodamine B > Cd (II) > Pb (II) > Cu (II) whereas the selectivity was shown to decrease the order of Cu (II) > Pb (II) > Cd (II) > Rhodamine B.

  1. Hg(II) sensing platforms with improved photostability: The combination of rhodamine derived chemosensors and up-conversion nanocrystals.

    Science.gov (United States)

    Song, Kai; Mo, Jingang; Lu, Chengwen

    2017-05-15

    This paper reported two nanocomposite sensing platforms for Hg(II) detection with improved photostability, using two rhodamine derivatives as chemosensors and up-conversion nanocrystals as excitation host, respectively. There existed a secondary energy transfer from this excitation host to these chemosensors, which was confirmed by spectral analysis, energy transfer radius calculation and emission decay lifetime comparison. In this case, chemosensor photostability was greatly improved. Further analysis suggested that these chemosensors recognized Hg(II) following a simple binding stoichiometry of 1:1. Hg(II) sensing performance of these sensing platforms was analyzed through their emission spectra upon various Hg(II) concentrations. Emission spectral response, Stern-Volmer equation, emission stability and sensing selectivity were discussed in detail. It was finally concluded that these chemosensors showed emission turn on effect towards Hg(II), with high photostability, good selectivity and linear response. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Giant unilamellar vesicles containing Rhodamine 6G as a marker for immunoassay of bovine serum albumin and lipocalin-2.

    Science.gov (United States)

    Sakamoto, Misato; Shoji, Atsushi; Sugawara, Masao

    2016-07-15

    Functionalized giant unilamellar vesicles (GUVs) containing a fluorescence dye Rhodamine 6G is proposed as a marker in sandwich-type immunoassay for bovine serum albumin (BSA) and lipocalin-2 (LCN2). The GUVs were prepared by the electroformation method and functionalized with anti-BSA antibody and anti-LCN2 antibody, respectively. The purification of antibody-modified GUVs was achieved by conventional centrifugation and a washing step in a flow system. To antigen on an antibody slip, antibody-modified GUVs were added as a marker and incubated. After wash-out of excess reagents and lysis of the bound GUVs with Triton X-100, the fluorescence image was captured. The fluorometric immunoassays for BSA and LCN2 exhibited lower detection limits of 4 and 80 fg ml(-)(1), respectively. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Sensitive detection of strong acidic condition by a novel rhodamine-based fluorescent pH chemosensor.

    Science.gov (United States)

    Tan, Jia-Lian; Yang, Ting-Ting; Liu, Yu; Zhang, Xue; Cheng, Shu-Jin; Zuo, Hua; He, Huawei

    2016-05-01

    A novel rhodamine-based fluorescent pH probe responding to extremely low pH values has been synthesized and characterized. This probe showed an excellent photophysical response to pH on the basis that the colorless spirocyclic structure under basic conditions opened to a colored and highly fluorescent form under extreme acidity. The quantitative relationship between fluorescence intensity and pH value (1.75-2.62) was consistent with the equilibrium equation pH = pKa + log[(Imax - I)/(I - Imin)]. This sensitive pH probe was also characterized with good reversibility and no interaction with interfering metal ions, and was successfully applied to image Escherichia coli under strong acidity. Copyright © 2015 John Wiley & Sons, Ltd.

  4. Monitoring of chlorsulfuron in biological fluids and water samples by molecular fluorescence using rhodamine B as fluorophore.

    Science.gov (United States)

    Alesso, Magdalena; Escudero, Luis A; Talio, María Carolina; Fernández, Liliana P

    2016-11-01

    A new simple methodology is proposed for chlorsufuron (CS) traces quantification based upon enhancement of rhodamine B (RhB) fluorescent signal. Experimental variables that influence fluorimetric sensitivity have been studied and optimized. The zeroth order regression calibration was linear from 0.866 to 35.800µgL(-1) CS, with a correlation coefficient of 0.99. At optimal experimental conditions, a limit of detection of 0.259µgL(-1) and a limit of quantification of 0.866µgL(-1) were obtained. The method showed good sensitivity and adequate selectivity and was applied to the determination of trace amounts of CS in plasma, serum and water samples with satisfactory results analyzed by ANOVA test. The proposed methodology represents an alternative to traditional chromatographic techniques for CS monitoring in complex samples, using an accessible instrument in control laboratories. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. A contribution to the study of radioactive waste dilution in the Rhone involving tests with a rhodamine B tracer

    International Nuclear Information System (INIS)

    Rodier, J.; Marichal, M.

    1961-01-01

    The process whereby waste from the Marcoule plant mixes with the water in the Rhone was followed in tests with rhodamine as a chemical tracer. Satisfactory dispersion was noted less than 4 km downstream from the waste discharge duct outlet, and the degree of homogeneity was considered to be satisfactory at the bridge of Roquemaure, und perfect at Avignon. This investigation not only revealed a complete absence of any preferential flow paths containing high radioactive waste concentrations, but it also enabled the most representative points to be selected at which to take Rhone water samples during future radioactive waste discharges. Reprint of a paper published in 'La Houille Blanche' N. 5 - Aug 196, p. 636-641 [fr

  6. Kinetic, Thermodynamic and Equilibrium Studies on Uptake of Rhodamine B onto ZnCl2 Activated Low Cost Carbon

    Directory of Open Access Journals (Sweden)

    N. Bhadusha

    2012-01-01

    Full Text Available A carbonaceous adsorbent prepared from biomass waste like wood apple outer shell (Limonia acidissima by ZnCl2 treatment was investigated for its efficiency in removing Rhodamine B (RDB. Influence of agitation time, adsorbent dose, dye concentration, pH and temperature were explored. Two theoretical adsorption isotherms namely Langmuir and Freundlich were used to describe the experimental results. The Langmuir adsorption capacity (Qo was found to be 46.7 mg/g and the equilibrium parameter (RL values indicate favourable adsorption. The experimental data were well fitted with Langmuir isotherm model and pseudo second order kinetic model. Desorption studies showed that ion exchange mechanism might be involved in the adsorption process.

  7. Enhanced chemiluminescence for trazodone trace analysis based on acidic permanganate oxidation in concurrent presence of rhodamine 6G.

    Science.gov (United States)

    Fujimori, Keiichi; Sakata, Yuta; Moriuchi-Kawakami, Takayo; Shibutani, Yasuhiko

    2017-11-01

    A new sensitized chemiluminescence method by acidic permanganate oxidation was developed for the sensitive determination of trazodone. A fluorescent dye as used rhodamine 6G to increase a chemiluminescence intensity. Under optimum conditions, the liner range of the calibration curve was obtained for 1-5000 nmol/L. The limit of detection was calculated from 3σ of a blank was 0.23 nmol/L. The coexistent ions and substances had no interference with the chemiluminescence measurement. The chemiluminescence spectra were measured to elucidate a possible mechanism for the system. The present method was satisfactorily used in the determination of the drugs in pharmaceutical samples and animal serums. Copyright © 2017 John Wiley & Sons, Ltd.

  8. Thermodynamic study of rhodamine 123-calf thymus DNA interaction: determination of calorimetric enthalpy by optical melting study.

    Science.gov (United States)

    Masum, Abdulla Al; Chakraborty, Maharudra; Pandya, Prateek; Halder, Umesh Chandra; Islam, Md Maidul; Mukhopadhyay, Subrata

    2014-11-20

    In this paper, the interaction of rhodamine123 (R123) with calf thymus DNA has been studied using molecular modeling and other biophysical methods like UV-vis spectroscopy, fluoremetry, optical melting, isothermal titration calorimetry, and circular dichroic studies. Results showed that the binding energy is about -6 to -8 kcal/mol, and the binding process is favored by both negative enthalpy change and positive entropy change. A new method to determine different thermodynamic properties like calorimetric enthalpy and heat capacity change has been introduced in this paper. The obtained data has been crossed-checked by other methods. After dissecting the free-energy contribution, it was observed that the binding was favored by both negative hydrophobic free energy and negative molecular free energy which compensated for the positive free energies due to the conformational change loss of rotational and transitional freedom of the DNA helix.

  9. A Continuous Flow System for the Measurement of Ambient Nitrogen Oxides [NO + NO] Using Rhodamine B Hydrazide as a Chemosensor

    Directory of Open Access Journals (Sweden)

    Pandurangappa Malingappa

    2014-01-01

    Full Text Available A new chemosensor has been used to monitor atmospheric nitrogen oxides [NO + NO 2 ] at parts per billion (ppb level. It is based on the catalytic reaction of nitrogen oxides with rhodamine B hydrazide (RBH to produce a colored compound through the hydrolysis of the amide bond of the molecule. A simple colorimeter has been used to monitor atmospheric nitrogen dioxide at ppb level. The air samples were purged through a sampling cuvette containing RBH solution using peristaltic pump. The proposed method has been successfully applied to monitor the ambient nitrogen dioxide levels at traffic junction points within the city limits and the results obtained are compared with the standard Griess-Ilosvay method.

  10. A new rhodamine-based fluorescent probe for the discrimination of Fe{sup 3+} from Fe{sup 2+}

    Energy Technology Data Exchange (ETDEWEB)

    You, Qi Hua; Huang, Hua Bin; Zhuang, Zhi Xia; Wang, Xiao Ru [Dept. of Science and Technology for Inspection, Xiamen Huaxia University, Xiamen (China); Chan, Wing Hong [Dept. of of Chemistry, Hong Kong Bap tist University, Hong Kong (China)

    2016-11-15

    A new rhodamine-based fluorescent probe for the discrimination of Fe{sup 3+} from Fe{sup 2+} has been designed and investigated. The probe shows an immediate visual color change in response to Fe{sup 3+} and Cu{sup 2+}, while only Fe{sup 3+} triggers the fluorescent change of the probe. The existence of large amount of other metal ions shows negligible interference in the detection of Fe{sup 3+}. The association constant K{sub ass} of 4.64 × 10{sup 8} M{sup -2} (R{sup 2} = 0.994) and 5.38 × 10{sup 8} M{sup -2} (R{sup 2} = 0.991) of the complex was derived from UV/Vis and fluorescence titration assuming 1:2 stoichiometry of probe–Fe{sup 3+} complex, respectively.

  11. Fluorescein isothiocyanate and rhodamine B dye encapsulated mesoporous SiO2 for applications of blue LED excited white LED

    Science.gov (United States)

    Das, Sourav; Manam, J.

    2018-05-01

    In this work, the fluorescein isothiocyanate (FITC) and rhodamine B (RhB) dyes were encapsulated in mesoporous silica nanoparticles (MSNp). The MSNp-FITC-RhB nanohybrids phosphor showed a dichromatic PL emission at green region and orange region when excited at 460 nm. A Forster Resonance Energy Transfer (FRET) was observed from FITC to RhB. The materials were further characterized by XRD, FTIR, TEM, and temperature dependent photoluminescence. The CIE coordinates were tuned from greenish yellow to the orange region and quantum yield was reached 52.04% based on FRET. So by combining the MSNp-FITC-RhB nanohybrids phosphor with the blue LED chip, the white light emission with flexible Color Correlated Temperature and improved Color Rendering Index can be obtained.

  12. Investigation of ionic associates of rare earths with 3,5-diiodosalicylic acid and rhodamine B as an analytical form

    Energy Technology Data Exchange (ETDEWEB)

    Tselik, E I; Kushch, V P; Poluehktov, N S; Mishchenko, V T [AN Ukrainskoj SSR, Kiev. Inst. Fizicheskoj Khimii

    1979-11-01

    It is established that three-charge ions of rare earth elements form ionic associates with 3,5-diiodosalicylic acid (DISA) and rhodamine B (RB) with the ratio of components Me:DISA:RB = 1:4:1. For the associates toluene is the most suitable solvent. Certain correlation dependencies of properties in the natural series of these elements are found. Extraction-photometric method of Nd and Er determination with sensitivity of 0.026 and 0.033 ..mu..g/ml respectively (l = 0.3 cm) and relative standard deviation of <= 0.043 is developed. The use of DISA permits to increase the method sensitivity as compared with the method in which salicylic acid is used.

  13. Synthesis and study of the optical properties of dielectric Bragg reflectors infiltrated with 6G-Rhodamine

    International Nuclear Information System (INIS)

    Gómez-Barojas, E; Aca-López, V; Luna-López, J A; Sánchez-Mora, E; Silva-González, R

    2014-01-01

    We report the study of the optical properties of 6G-Rhodamine (Rhd) infiltrated porous silicon dielectric Bragg reflectors (DBRs) with 31 constituent periods. The DBRs were obtained by an electrochemical anodizing process of Si in a two electrodes Teflon cell. The porosity was determined by gravimetric measurements on single Porous silicon (PSi) layers. Based on the characterization results of single layers the DBRs were synthesized. After anodizing, the DBRs were silanized with a 3-mercaptopropyltrimethoxysilane solution and functionalized with Rhd solutions at different concentrations. Cross section scanning electron micrographs show that the DBRs synthesis was successful. After each preparation step, Reflectance and Fluorescence (FL) spectra were recorded. These spectra show that as the Rhd concentration in solution is increased the stop band intensity as well as the FL intensity are enhanced due to constructive interference effects

  14. Fabrication and characterization of microcavity lasers in rhodamine B doped SU8 using high energy proton beam

    Science.gov (United States)

    Venugopal Rao, S.; Bettiol, A. A.; Vishnubhatla, K. C.; Bhaktha, S. N. B.; Narayana Rao, D.; Watt, F.

    2007-03-01

    The authors present their results on the characterization of individual dye-doped microcavity polymer lasers fabricated using a high energy proton beam. The lasers were fabricated in rhodamine B doped SU8 resist with a single exposure step followed by chemical processing. The resulting trapezoidal shaped cavities had dimensions of ˜250×250μm2. Physical characterization of these structures was performed using a scanning electron microscope while the optical characterization was carried out by recording the emission subsequent to pumping the lasers with 532nm, 6 nanosecond pulses. The authors observed intense, narrow emission near 624nm with the best emission linewidth full width at half maximum of ˜9nm and a threshold ˜150μJ/mm2.

  15. Characterization of channel waveguides and tunable microlasers in SU8 doped with rhodamine B fabricated using proton beam writing

    International Nuclear Information System (INIS)

    Rao, S Venugopal; Bettiol, A A; Watt, F

    2008-01-01

    We present our results on the fabrication and characterization of buried channel waveguides and tunable microlasers in SU8 doped with rhodamine B achieved using direct writing with a 2.0 MeV proton beam. The channel waveguides, fabricated in single exposure, had an optical propagation loss of -1 at 532 nm measured using the scattering technique while the microlasers with dimensions of 250 x 250 μm 2 had a threshold of ∼150 μJ mm -2 when pumped with 532 nm nanosecond pulses. The emitted wavelength from the microlasers was tunable to an extent of ∼15 nm with increasing pump intensity and different pumping angles. The advantages of such micro-photonic components for the realization of a lab-on-a-chip device are discussed briefly. (fast track communication)

  16. A rhodamine chromene-based turn-on fluorescence probe for selectively imaging Cu2+ in living cell

    Science.gov (United States)

    Liu, Wei-Yong; Li, Hai-Ying; Lv, Hong-Shui; Zhao, Bao-Xiang; Miao, Jun-Ying

    We describe the development of a rhodamine chromene-based turn-on fluorescence probe to monitor the intracellular Cu2+ level in living cells. The new fluorescent probe with a chlorine group in chromene moiety exhibits good membrane-permeable property than previous reported because the predicted lipophilicity of present probe 4 is stronger than that of methoxyl substituted probe in our previous work (CLogP of 4: 8.313, CLogP of methoxyl substituted probe: 7.706), and a fluorescence response toward Cu2+ under physiological conditions with high sensitivity and selectivity, and facilitates naked-eye detection of Cu2+. The fluorescence intensity was remarkably increased upon the addition of Cu2+ within 1 or 2 min, while the other sixteen metal ions caused no significant effect.

  17. Electrochemical sensors based on gold nanoparticles modified with rhodamine B hydrazide to sensitively detect Cu(II)

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Donglai; Hu, Bin; Kang, Mengmeng [Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001 (China); Wang, Minghua [Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, No.136, Science Avenue, Zhengzhou 450001 (China); He, Linghao [Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001 (China); Zhang, Zhihong, E-mail: mainzhh@163.com [Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001 (China); Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, No.136, Science Avenue, Zhengzhou 450001 (China); Fang, Shaoming, E-mail: mingfang@zzuli.edu.cn [Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001 (China); Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, No.136, Science Avenue, Zhengzhou 450001 (China)

    2016-12-30

    Highlights: • An electrochemical sensor based on gold nanoparticles modified with rhodamine B hydrazide (AuNPs-RBH) was developed. • The sensor was applied in the highly sensitive and selective detection of Cu{sup 2+} in water. • The electrochemical sensor displays excellent regeneration, stability, and practicability for Cu{sup 2+} detection. • EIS was used to determine Cu{sup 2+} ions in an aqueous solution with the developed AuNPs-RBH-based electrochemical sensor. - Abstract: An electrochemical sensor based on gold nanoparticles (Au NPs) modified with rhodamine B hydrazide (RBH) (AuNPs-RBH) was developed and applied in the highly sensitive and selective detection of Cu{sup 2+} in water. RBH molecules were bounded onto the surface of AuNPs via the strong interaction between the amino groups and Au NPs. The chemical structure variations were characterized by X-ray photoelectron spectroscopy and fluoresence spectroscopy. Additionally, electrochemical impedance spectroscopy was used to determine Cu{sup 2+} ions in an aqueous solution with the developed AuNPs-RBH-based electrochemical sensor. Results show that the fabricated sensor exhibits good electrochemical performance because of the presence of Au NPs and high affinity with the Cu{sup 2+} resulting from the strong coordination chemistry between Cu{sup 2+} and RBH. The as-developed sensor towards detecting Cu{sup 2+} has a detection limitation of 12.5 fM within the concentration range of 0.1 pM–1 nM by using the electrochemical impedance technique. It also displays excellent selectivity, regeneration, stability, and practicability for Cu{sup 2+} detection. Therefore, the new strategy of the RBH-based electrochemical sensor exhibits great potential application in environment treatment and protection.

  18. Composite Magnetic Nanoparticles (CuFe₂O₄) as a New Microsorbent for Extraction of Rhodamine B from Water Samples.

    Science.gov (United States)

    Roostaie, Ali; Allahnoori, Farzad; Ehteshami, Shokooh

    2017-09-01

    In this work, novel composite magnetic nanoparticles (CuFe2O4) were synthesized based on sol-gel combustion in the laboratory. Next, a simple production method was optimized for the preparation of the copper nanoferrites (CuFe2O4), which are stable in water, magnetically active, and have a high specific area used as sorbent material for organic dye extraction in water solution. CuFe2O4 nanopowders were characterized by field-emission scanning electron microscopy (SEM), FTIR spectroscopy, and energy dispersive X-ray spectroscopy. The size range of the nanoparticles obtained in such conditions was estimated by SEM images to be 35-45 nm. The parameters influencing the extraction of CuFe2O4 nanoparticles, such as desorption solvent, amount of sorbent, desorption time, sample pH, ionic strength, and extraction time, were investigated and optimized. Under the optimum conditions, a linear calibration curve in the range of 0.75-5.00 μg/L with R2 = 0.9996 was obtained. The LOQ (10Sb) and LOD (3Sb) of the method were 0.75 and 0.25 μg/L (n = 3), respectively. The RSD for a water sample spiked with 1 μg/L rhodamine B was 3% (n = 5). The method was applied for the determination of rhodamine B in tap water, dishwashing foam, dishwashing liquid, and shampoo samples. The relative recovery percentages for these samples were in the range of 95-99%.

  19. A novel magnetic poly(aniline-naphthylamine)-based nanocomposite for micro solid phase extraction of rhodamine B

    International Nuclear Information System (INIS)

    Bagheri, Habib; Daliri, Rasoul; Roostaie, Ali

    2013-01-01

    Graphical abstract: -- Highlights: •A Fe 3 O 4 –aniline-naphthylamine nanocomposite was prepared via a simple route. •The magnetic nanocomposite was applied for isolation of RhB from water. •The nanocomposite applicability was compared with other pristine polymers. •The method was applied for the determination of RhB in different samples. -- Abstract: A novel Fe 3 O 4 –poly(aniline-naphthylamine)-based nanocomposite was synthesized by chemical oxidative polymerization process as a magnetic sorbent for micro solid phase extraction. The scanning electron microscopy images of the synthesized nanocomposite revealed that the copolymer posses a porous structure with diameters less than 50 nm. The extraction efficiency of this sorbent was examined by isolation of rhodamine B, a mutagenic and carcinogenic dye, from aquatic media in dispersion mode. Among different synthesized polymers, Fe 3 O 4 /poly(aniline-naphthylamine) nanocomposite showed a prominent efficiency. Parameters including the desorption solvent, amount of sorbent, desorption time, sample pH, ionic strength, extraction time and stirring rate were optimized. Under the optimum condition, a linear spiked calibration curve in the range of 0.35–5.00 μg L −1 with R 2 = 0.9991 was obtained. The limits of detection (3S b ) and limits of quantification (10S b ) of the method were 0.10 μg L −1 and 0.35 μg L −1 (n = 3), respectively. The relative standard deviation for water sample with 0.5 μg L −1 of RhB was 4.2% (n = 5) and the absolute recovery was 92%. The method was applied for the determination of rhodamine B in dishwashing foam, dishwashing liquid, shampoo, pencil, matches tips and eye shadows samples and the relative recovery percentage were in the range of 94–99%

  20. Synthesis, surface group modification of 3D MnV2O6 nanostructures and adsorption effect on Rhodamine B

    International Nuclear Information System (INIS)

    Zhang, Wanqun; Shi, Lei; Tang, Kaibin; Liu, Zhongping

    2012-01-01

    Highlights: ► Fabrication of urchin-like MnV 2 O 6 with oxygen-containing surface groups. ► Mn 0.5 V 2 O 5 ·nH 2 O as an intermediate product holds the key to the final products. ► 3D architectures of MnV 2 O 6 with oxygen-containing surface groups as sorbent. ► The sorbent shows a good adsorption ability. -- Abstract: Highly uniform 3D MnV 2 O 6 nanostructures modified by oxygen functional groups (-COO-) were successfully prepared in large quantities by an approach involving preparation of vanadyl ethylene glycolate as the precursor. The growth and self-assembly of MnV 2 O 6 nanobelts and nanorods could be readily tuned by additive species and quantities, which brought different morphologies and sizes to the final products. With a focus on the regulation of structure, the formation process of 3D architectures of MnV 2 O 6 by self-assembly of nanobelts was followed by field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD). The consecutive processes of vanadyl ethylene glycolate and benzoyl peroxide assisted formation of layered structure Mn 0.5 V 2 O 5 ·nH 2 O, growth of aligned MnV 2 O 6 nanobelts, and oriented assembly were proposed for the growth mechanism. The band gap vs. different morphology was also studied. Optical characterization of these MnV 2 O 6 with different morphologies showed direct bandgap energies at 1.8–1.95 eV. The adsorption properties of 3D MnV 2 O 6 nanostructures synthesized under different conditions were investigated through the removal test of Rhodamine B in aqueous water, and the 3D nanostructures synthesized with 30 g L −1 benzoyl peroxide showed good adsorption capability of Rhodamine B.

  1. Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.

    Science.gov (United States)

    Johnson, Brian J; Mitchell, Sara N; Paton, Christopher J; Stevenson, Jessica; Staunton, Kyran M; Snoad, Nigel; Beebe, Nigel; White, Bradley J; Ritchie, Scott A

    2017-09-01

    Recent interest in male-based sterile insect technique (SIT) and incompatible insect technique (IIT) to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success) during field releases. Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v) in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males. These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis) and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males.

  2. Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.

    Directory of Open Access Journals (Sweden)

    Brian J Johnson

    2017-09-01

    Full Text Available Recent interest in male-based sterile insect technique (SIT and incompatible insect technique (IIT to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success during field releases.Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males.These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males.

  3. Real-time piscicide tracking using Rhodamine WT dye for support of application, transport, and deactivation strategies in riverine environments

    Science.gov (United States)

    Jackson, Patrick Ryan; Lageman, Jonathan D.

    2013-01-01

    Piscicide applications in riverine environments are complicated by the advection and dispersion of the piscicide by the flowing water. Proper deactivation of the fish toxin is required outside of the treatment reach to ensure that there is minimal collateral damage to fisheries downstream or in connecting and adjacent water bodies. In urban settings and highly managed waterways, further complications arise from the influence of industrial intakes and outfalls, stormwater outfalls, lock and dam operations, and general unsteady flow conditions. These complications affect the local hydrodynamics and ultimately the transport and fate of the piscicide. This report presents two techniques using Rhodamine WT dye for real-time tracking of a piscicide plume—or any passive contaminant—in rivers and waterways in natural and urban settings. Passive contaminants are those that are present in such low concentration that there is no effect (such as buoyancy) on the fluid dynamics of the receiving water body. These methods, when combined with data logging and archiving, allow for visualization and documentation of the application and deactivation process. Real-time tracking and documentation of rotenone applications in rivers and urban waterways was accomplished by encasing the rotenone plume in a plume of Rhodamine WT dye and using vessel-mounted submersible fluorometers together with acoustic Doppler current profilers (ADCP) and global positioning system (GPS) receivers to track the dye and map the water currents responsible for advection and dispersion. In this study, two methods were used to track rotenone plumes: (1) simultaneous injection of dye with rotenone and (2) delineation of the upstream and downstream boundaries of the treatment zone with dye. All data were logged and displayed on a shipboard laptop computer, so that survey personnel provided real-time feedback about the extent of the rotenone plume to rotenone application and deactivation personnel. Further

  4. Synthesis and semiconducting properties of tin(II) sulfide: Application to photocatalytic degradation of Rhodamine B under sun light

    Energy Technology Data Exchange (ETDEWEB)

    Kabouche, S. [Laboratory of Electrochemistry-Corrosion, Metallurgy and Inorganic Chemistry, Faculty of Chemistry, U.S.T.H.B., BP 32, Algiers, 16111 (Algeria); Bellal, B. [Laboratory of Storage and Valorization of Renewable Energies, Faculty of the Chemistry, U.S.T.H.B., BP 32, Algiers, 16111 (Algeria); Louafi, Y. [Laboratory of Electrochemistry-Corrosion, Metallurgy and Inorganic Chemistry, Faculty of Chemistry, U.S.T.H.B., BP 32, Algiers, 16111 (Algeria); Trari, M., E-mail: solarchemistry@gmail.com [Laboratory of Storage and Valorization of Renewable Energies, Faculty of the Chemistry, U.S.T.H.B., BP 32, Algiers, 16111 (Algeria)

    2017-07-01

    We have investigated the semiconducting and photoelectrochemical properties of SnS grown by a template-free chemical route using thiourea as precursor. Tin(II) sulfide is characterized by X-ray diffraction, scanning electron microscopy, diffuse reflectance and Raman spectroscopy. The X-ray diffraction indicates an orthorhombic SnS phase (SG: Pbnm) with a crystallite size of 52 nm while the optical measurements give a direct band gap of 1.33 eV. The Mott–Schottky plot exhibits a linear behavior, characteristic of n-type conductivity with a flat band potential of 0.19 V{sub SCE} and a donor density of 4.12 × 10{sup 18} cm{sup -3}. The electrochemical impedance spectroscopy (EIS) measured in the range (10{sup -2}–5 × 10{sup 4} Hz) shows one semicircle attributed to the bulk resistance (R{sub b} = 20.37 kΩ cm{sup 2}). The conduction band, located at 4.84 eV below vacuum, is made up of Sn{sup 2+:}5p while the valence band (6.17 eV) derives mainly from S{sup 2-}: 3p character. The energy band diagram, constructed from the photoelectrochemical characterization, predicts the photodegradation of Rhodamine B on SnS by H{sub 2}O{sub 2} generated photoelectrochemically. 88.46% of the initial concentration (10 mg L{sup -1}) disappears after adsorption and 4 h of exposure to solar light. The photoactivity is nearly restored during the second cycle and follows a second order kinetic with a rate constant of 1.55 × 10{sup -3} mg{sup -1} L min{sup -1}. - Highlights: • The semiconducting properties of SnS synthesized by chemical route are studied. • The n type conductivity is evidenced by chrono-amperometry and photoelectrochemistry. • The conduction band, located at 4.84 eV below vacuum, is made up of Sn{sup 2+}: 5p. • SnS was successfully used for the Rhodamine B oxidation under sunlight.

  5. A novel magnetic poly(aniline-naphthylamine)-based nanocomposite for micro solid phase extraction of rhodamine B

    Energy Technology Data Exchange (ETDEWEB)

    Bagheri, Habib, E-mail: bagheri@sharif.edu; Daliri, Rasoul; Roostaie, Ali

    2013-09-10

    Graphical abstract: -- Highlights: •A Fe{sub 3}O{sub 4}–aniline-naphthylamine nanocomposite was prepared via a simple route. •The magnetic nanocomposite was applied for isolation of RhB from water. •The nanocomposite applicability was compared with other pristine polymers. •The method was applied for the determination of RhB in different samples. -- Abstract: A novel Fe{sub 3}O{sub 4}–poly(aniline-naphthylamine)-based nanocomposite was synthesized by chemical oxidative polymerization process as a magnetic sorbent for micro solid phase extraction. The scanning electron microscopy images of the synthesized nanocomposite revealed that the copolymer posses a porous structure with diameters less than 50 nm. The extraction efficiency of this sorbent was examined by isolation of rhodamine B, a mutagenic and carcinogenic dye, from aquatic media in dispersion mode. Among different synthesized polymers, Fe{sub 3}O{sub 4}/poly(aniline-naphthylamine) nanocomposite showed a prominent efficiency. Parameters including the desorption solvent, amount of sorbent, desorption time, sample pH, ionic strength, extraction time and stirring rate were optimized. Under the optimum condition, a linear spiked calibration curve in the range of 0.35–5.00 μg L{sup −1} with R{sup 2} = 0.9991 was obtained. The limits of detection (3S{sub b}) and limits of quantification (10S{sub b}) of the method were 0.10 μg L{sup −1} and 0.35 μg L{sup −1} (n = 3), respectively. The relative standard deviation for water sample with 0.5 μg L{sup −1} of RhB was 4.2% (n = 5) and the absolute recovery was 92%. The method was applied for the determination of rhodamine B in dishwashing foam, dishwashing liquid, shampoo, pencil, matches tips and eye shadows samples and the relative recovery percentage were in the range of 94–99%.

  6. Pyrazolone as a recognition site: Rhodamine 6G-based fluorescent probe for the selective recognition of Fe3+ in acetonitrile-aqueous solution.

    Science.gov (United States)

    Parihar, Sanjay; Boricha, Vinod P; Jadeja, R N

    2015-03-01

    Two novel Rhodamine-pyrazolone-based colorimetric off-on fluorescent chemosensors for Fe(3+) ions were designed and synthesized using pyrazolone as the recognition moiety and Rhodamine 6G as the signalling moiety. The photophysical properties and Fe(3+) -binding properties of sensors L(1) and L(2) in acetonitrile-aqueous solution were also investigated. Both sensors successfully exhibit a remarkably 'turn-on' response, toward Fe(3+) , which was attributed to 1: 2 complex formation between Fe(3+) and L(1) /L(2) . The fluorescent and colorimetric response to Fe(3+) can be detected by the naked eye, which provides a facile method for the visual detection of Fe(3+) . Copyright © 2014 John Wiley & Sons, Ltd.

  7. Photocatalytic properties of BiVO{sub 4} prepared by the co-precipitation method: Degradation of rhodamine B and possible reaction mechanisms under visible irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Martinez-de la Cruz, A., E-mail: azael70@yahoo.com.mx [Facultad de Ingenieria Mecanica y Electrica, Universidad Autonoma de Nuevo Leon, Ciudad Universitaria, C.P. 66451, San Nicolas de los Garza, N.L. (Mexico); Perez, U.M. Garcia [Facultad de Ingenieria Mecanica y Electrica, Universidad Autonoma de Nuevo Leon, Ciudad Universitaria, C.P. 66451, San Nicolas de los Garza, N.L. (Mexico)

    2010-02-15

    Bismuth vanadate (BiVO{sub 4}) was synthesized by the co-precipitation method at 200 {sup o}C. The photocatalytic activity of the oxide was tested for the photodegradation of rhodamine B under visible light irradiation. The analysis of the total organic carbon showed that the mineralization of rhodamine B over a BiVO{sub 4} photocatalyst ({approx}40% after 100 h of irradiation) is feasible. In the same way, a gas chromatography analysis coupled with mass spectroscopy revealed the existence of organic intermediates during the photodegradation process such as ethylbenzene, o-xylene, m-xylene, and phthalic anhydride. The modification of variables such as dispersion pH, amount of dissolved O{sub 2}, and irradiation source was studied in order to know the details about the photodegradation mechanism.

  8. Photocatalytic properties of BiVO4 prepared by the co-precipitation method: Degradation of rhodamine B and possible reaction mechanisms under visible irradiation

    International Nuclear Information System (INIS)

    Martinez-de la Cruz, A.; Perez, U.M. Garcia

    2010-01-01

    Bismuth vanadate (BiVO 4 ) was synthesized by the co-precipitation method at 200 o C. The photocatalytic activity of the oxide was tested for the photodegradation of rhodamine B under visible light irradiation. The analysis of the total organic carbon showed that the mineralization of rhodamine B over a BiVO 4 photocatalyst (∼40% after 100 h of irradiation) is feasible. In the same way, a gas chromatography analysis coupled with mass spectroscopy revealed the existence of organic intermediates during the photodegradation process such as ethylbenzene, o-xylene, m-xylene, and phthalic anhydride. The modification of variables such as dispersion pH, amount of dissolved O 2 , and irradiation source was studied in order to know the details about the photodegradation mechanism.

  9. A ratiometric fluorescent probe based on boron dipyrromethene and rhodamine Förster resonance energy transfer platform for hypochlorous acid and its application in living cells

    International Nuclear Information System (INIS)

    Liu, Ying; Zhao, Zhi-Min; Miao, Jun-Ying; Zhao, Bao-Xiang

    2016-01-01

    We have developed a ratiometric fluorescent probe BRT based on boron dipyrromethene (BODIPY) and rhodamine-thiohydrazide Förster resonance energy transfer (FRET) platform for sensing hypochlorous acid (HOCl) with high selectivity and sensitivity. The probe can detect HOCl in 15 s with the detection limit of 38 nM. Upon mixing with HOCl the fluorescence colour of probe BRT changed from green to orange. Moreover, probe BRT was applied to successfully monitor HOCl in living RAW 264.7 cells. - Highlights: • A probe based on BODIPY and rhodamine was developed for sensing HOCl. • The probe could sense HOCl in a ratiometric manner based on the FRET platform in PBS buffer solution. • The probe can detect HOCl in 15 s accompanied with a fluorescence colour change. • This probe was successfully used to monitor HOCl in living RAW 264.7 cells.

  10. A validated LC-MS/MS determination method for the illegal food additive rhodamine B: Applications of a pharmacokinetic study in rats.

    Science.gov (United States)

    Cheng, Yung-Yi; Tsai, Tung-Hu

    2016-06-05

    Rhodamine B is an illegal and potentially carcinogenic food dye. The aim of this study was to develop a convenient, rapid, and sensitive UHPLC-MS/MS method for pharmacokinetic studies in rats. Rat plasma samples were deproteinized with acetonitrile and separated by UHPLC on a reverse-phase C18e column (100mm×2.1mm, 2μm) using a mobile phase consisting of methanol-5mM ammonium acetate (90:10, v/v). Detection was performed using a triple quadrupole tandem mass spectrometer in the selected reaction monitoring mode at [M](+) ion m/z 443.39→399.28 for rhodamine B and [M+H](+) ion m/z 253.17→238.02 for 5-methoxyflavone as the internal standard. This method was specific and produced linear results over a concentration range of 0.5-100ng/mL, with a lower limit of quantitation of 0.5ng/mL. All validation parameters, including the inter-day, intra-day, matrix effect, recovery, and stability in rat plasma, were acceptable according to the biological method validation guidelines developed by the FDA (2001). This method was successfully applied to a pharmacokinetic study in rats; oral administration of 1mg/kg of rhodamine B yielded a time to maximum concentration (Tmax) of 1.3±0.4h and an elimination half-life of 8.8±1.4h, with a clearance of 229.7±19.4mL/h/kg. These pharmacokinetic results provide a constructive contribution to our understanding of the absorption mechanism of rhodamine B and support additional food safety evaluations. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Plasmon enhancement of Raman scattering and fluorescence for rhodamine 6G molecules in the porous glass and PVA films with nanoparticles of silver citrate hydrosol

    International Nuclear Information System (INIS)

    Konstantinova, E I; Zyubin, A U; Samusev, I G; Slezhkin, V A; Bryukhanov, V V

    2016-01-01

    The study of Raman and fluorescence spectra for Rhodamine 6G molecules in a film of polyvinyl alcohol on the modified by silver nanoparticles (NPs) porous glass and without the porous glass has been done. The gain of the scattering intensity and fluorescence emission has been obtained in the presence of silver nanoparticles. The gain order was obtained as ∼ 10"1"1 (paper)

  12. Sensitive and selective determination of fluvoxamine maleate using a sensitive chemiluminescence system based on the alkaline permanganate-Rhodamine B-gold nanoparticles reaction.

    Science.gov (United States)

    Hassanzadeh, Javad; Amjadi, Mohammad

    2015-06-01

    A high-yield chemiluminescence (CL) system based on the alkaline permanganate-Rhodamine B reaction was developed for the sensitive determination of fluvoxamine maleate (Flu). Rhodamine B is oxidized by alkaline KMnO4 and a weak CL emission is produced. It was demonstrated that gold nanoparticles greatly enhance this CL emission due to their interaction with Rhodamine B molecules. It is also observed that sodium dodecyl sulfate, an anionic surfactant, can strongly increase this enhancement. In addition, it was demonstrated that a notable decrease in the CL intensity is observed in the presence of Flu. This may be related to Flu oxidation with KMnO4 . There is a linear relationship between the decrease in CL intensity and the Flu concentration over a range of 2-300 µg/L. A new simple, rapid and sensitive CL method was developed for the determination of Flu with a detection limit (3s) of 1.35 µg/L. The proposed method was used for the determination of Flu in pharmaceutical and urine samples. Copyright © 2014 John Wiley & Sons, Ltd.

  13. Effect of the prosthetic group on the pharmacologic properties of 18F-labeled rhodamine B, a potential myocardial perfusion agent for positron emission tomography (PET).

    Science.gov (United States)

    Bartholomä, Mark D; Gottumukkala, Vijay; Zhang, Shaohui; Baker, Amanda; Dunning, Patricia; Fahey, Frederic H; Treves, S Ted; Packard, Alan B

    2012-12-27

    We recently reported the development of the 2-[(18)F]fluoroethyl ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging. This compound, which was prepared using a [(18)F]fluoroethyl prosthetic group, has significant uptake in the myocardium in rats but also demonstrates relatively high liver uptake and is rapidly hydrolyzed in vivo in mice. We have now prepared (18)F-labeled rhodamine B using three additional prosthetic groups (propyl, diethylene glycol, and triethylene glycol) and found that the prosthetic group has a significant effect on the in vitro and in vivo properties of these compounds. Of the esters prepared to date, the diethylene glycol ester is superior in terms of in vitro stability and pharmacokinetics. These observations suggest that the prosthetic group plays a significant role in determining the pharmacological properties of (18)F-labeled compounds. They also support the value of continued investigation of (18)F-labeled rhodamines as PET radiopharmaceuticals for myocardial perfusion imaging.

  14. The Effect of the Prosthetic Group on the Pharmacologic Properties of 18F-labeled Rhodamine B, a Potential Myocardial Perfusion Agent for PET

    Science.gov (United States)

    Bartholomä, Mark D.; Gottumukkala, Vijay; Zhang, Shaohui; Baker, Amanda; Dunning, Patricia; Fahey, Frederic H.; Treves, S. Ted; Packard, Alan B.

    2013-01-01

    We recently reported the development of the 2-[18F]fluoroethyl ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging. This compound, which was prepared using a [18F]fluoroethyl prosthetic group, has significant uptake in the myocardium in rats, but also demonstrates relatively high liver uptake and is rapidly hydrolyzed in vivo in mice. We have now prepared 18F-labeled rhodamine B using three additional prosthetic groups (propyl, diethylene glycol, and triethylene glycol) and found that the prosthetic group has a significant effect on the in vitro and in vivo properties of these compounds. Of the esters prepared to date, the diethylene glycol ester is superior in terms of in vitro stability and pharmacokinetics. These observations suggest that the prosthetic group plays a significant role in determining the pharmacological properties of 18F-labeled compounds. They also support the value of continued investigation of 18F-labeled rhodamines as PET radiopharmaceuticals for myocardial perfusion imaging. PMID:23210516

  15. Picosecond studies of excitation transport in a finite volume: The clustered transport system octadecyl rhodamine B in triton X-100 micelles

    International Nuclear Information System (INIS)

    Ediger, M.D.; Domingue, R.P.; Fayer, M.D.

    1984-01-01

    A detailed experimental and theoretical examination of electronic excited state transport in the finite volume system, octadecyl rhodamine B molecules in triton X-100 micelles, is presented. Picosecond fluorescence mixing and transient grating techniques were used to examine systems in which the average number of chromophores per micelle ranged from 0.1 to 11. Because of the clustering of chromophores in the small micelles, the energy transport observed is extremely efficient. A statistical mechanical theory, based on a density expansion with a Pade approximant, is developed for donor--donor transport on a spherical surface. This theory accurately accounts for the experimental data with only the micelle radius as an adjustable parameter. The radius obtained from this procedure is in good agreement with determinations by other methods. This demonstrates that quantitative information about the spatial extent of chromophore distributions in small volumes can be obtained when appropriate finite volume energy transport theories are employed. It is shown that theories developed for infinite volumes are not applicable to systems such as the ones considered here. Finally the partitioning of rhodamine B and octadecyl rhodamine B between aqueous and micellar phases is measured, and lifetimes and rotation times are reported

  16. Photocatalytic Degradation of Rhodamine B Dye over Novel Porous TiO2-SnO2 Nanocomposites Prepared by Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    Yan Wang

    2014-01-01

    Full Text Available The photocatalytic degradation of Rhodamine B dye was successfully carried out under UV irradiation over porous TiO2-SnO2 nanocomposites with various molar ratios of Ti/Sn (4–12 synthesized by hydrothermal method using polystyrene microspheres as template. The combination of TiO2 with SnO2 can obtain high quantum yield of TiO2, and then achieve the high photocatalytic activity. And its porous structure can provide large surface area, leading to more adsorption and fast transfer of dye pollutant. Structural and textural features of the samples were investigated by X-ray diffraction (XRD, transmission electron microscopy (TEM, and N2 sorption techniques. Both adsorption and UV irradiation contribute to decolorization of about 100% of Rhodamine B dye over the sample TiSn10 after 30 min of the photocatalytic reaction, while the decomposition of Rhodamine B dye is only 62% over pure titania (Degussa P25.

  17. Adsorption of rhodamine B and methylene blue dyes using waste of seeds of Aleurites Moluccana, a low cost adsorbent

    Directory of Open Access Journals (Sweden)

    Debora Luiza Postai

    2016-06-01

    Full Text Available Removal of the cationic dyes rhodamine B (RhB and methylene blue (MB by waste seeds Aleurites moluccana (WAM was studied in a batch system. The adsorbent was characterized by Fourier transform infrared spectroscopy (FTIR, thermogravimetric analysis (TGA, point of zero charge measurement, and the Boehm titration method. The effects of contact time and pH were investigated for the removal of cationic dyes. An increase in pH from 3 to 9 was accompanied by an approximately three-fold increase in the amount of dye adsorbed. The adsorptions equilibrium values were obtained and analyzed using the Langmuir, Freundlich, Sips, and Redlich–Peterson equations, the Sips isotherm being the one that showed the best correlation with the experimental values. The maximum adsorption capacities of the dyes were 178 mg/g for the MB and 117 mg/g for the RhB. The kinetic sorption was evaluated by the pseudo-first-order, pseudo-second-order, and intraparticle diffusion models, where it was observed that sorption follows the pseudo-second-order kinetic model. The study of thermodynamics showed that the adsorption is a spontaneous and endothermic process. The results indicate that waste seeds of A. moluccana could be used as a low cost material for the removal of cationic dyes from wastewater.

  18. Enhancing the photovoltaic performance of bulk heterojunction polymer solar cells by adding Rhodamine B laser dye as co-sensitizer.

    Science.gov (United States)

    Kazemifard, Sholeh; Naji, Leila; Afshar Taromi, Faramarz

    2018-04-01

    Ternary blend (TB) strategy has been considered as an effective method to enhance the photovoltaic performance of bulk heterojunction (BHJ) polymer solar cells (PSCs). Here, we report on TB-based PSCs containing two donor materials; poly-3-hexylthiophene (P3HT) and Rhodamine B (RhB) laser organic dye, and [6,6]-phenyl C 61 butyric acid methyl ester (PC 61 BM) as an acceptor. The influence of RhB weight percentage and injection volume was extensively studied. To gain insight into the influences of RhB on the photovoltaic performance of PSCs, physicochemical and optical properties of TBs were compared with those of BHJ binary blend as a standard. RhB broadened the light absorption properties of the active layer and played a bridging role between P3HT and PC 61 BM. The PCE and short-circuit current density (Jsc) of the optimized TB-based PSCs comprising of 0.5 wt% RhB reached 5% and 12.12 mA/cm 2 , respectively. Compared to BHJ standard cell, the PCE and the generated current was improved by two orders of magnitude due to higher photon harvest of the active layer, cascade energy level structure of TB components and a considerable decrease in the charge carrier recombination. The results suggest that RhB can be considered as an effective material for application in PSCs to attain high photovoltaic performance. Copyright © 2018 Elsevier Inc. All rights reserved.

  19. Photoluminescence Spectroscopy of Rhodamine 800 Aqueous Solution and Dye-Doped Polymer Thin-Film: Concentration and Solvent Effects

    Science.gov (United States)

    Le, Khai Q.; Dang, Ngo Hai

    2018-05-01

    This paper investigates solvent and concentration effects on photoluminescence (PL) or fluorescence properties of Rhodamine 800 (Rho800) dyes formed in aqueous solution and polymer thin-film. Various commonly used organic solvents including ethanol, methanol and cyclopentanol were studied at a constant dye concentration. There were small changes in the PL spectra for the different solvents in terms of PL intensity and peak wavelength. The highest PL intensity was observed for cyclopentanol and the lowest for ethanol. The longest peak wavelength was found in cyclopentanol (716 nm) and the shortest in methanol (708 nm). Dissolving the dye powder in the methanol solvent and varying the dye concentration in aqueous solution from the high concentrated solution to highly dilute states, the wavelength tunability was observed between about 700 nm in the dilute state and 730 nm at high concentration. Such a large shift may be attributed to the formation of dye aggregates. Rho800 dye-doped polyvinyl alcohol (PVA) polymer thin-film was further investigated. The PL intensity of the dye in the form of thin-film is lower than that of the aqueous solution form whereas the peak wavelength is redshifted due to the presence of PVA. This paper, to our best knowledge, reports the first study of spectroscopic properties of Rho800 dyes in various forms and provides useful guidelines for production of controllable organic luminescence sources.

  20. The transfer of natural Rhodamine B contamination from raw paprika fruit to capsicum oleoresin during the extraction process.

    Science.gov (United States)

    Wu, Naiying; Gao, Wei; Lian, Yunhe; Du, Jingjing; Tie, Xiaowei

    2017-12-15

    Occurrence of Rhodamine B (RhB) contamination in paprika caused by agricultural materials during the vegetation process has been reported. It may transfer during the process of active compounds extraction, and eventually exist in final products. Herein, the re-distribution of RhB during the extraction process was assessed in terms of RhB contents, as well as mass, color value and capsaicinoids yield of each process. Results revealed that natural RhB contamination at 0.55-1.11µg/kg originated from raw paprika fruit then transferred with the extraction proceeded. About 95.5% of RhB was found in red oleoresin. After separation of red oleoresin, 91.6% of RhB was remained in capsicum oleoresin, only 3.7% in paprika red. These results were consistent with total capsaicinoids recovery of each product. The RhB levels in edible capsicum oleoresin in our present study at 0.01-0.34µg/kg did not exceed the legal limits established by the European Union. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. A highly selective fluorescent chemosensor for Cu2+ : synthesis and properties of a rhodamine B-containing diarylethene.

    Science.gov (United States)

    Xue, Dandan; Zheng, Chunhong; Qu, Shengzu; Liao, Guanming; Fan, Congbin; Liu, Gang; Pu, Shouzhi

    2017-06-01

    A diarylethene bearing a triazole-linked rhodamine B unit was synthesized. Its fluorescent emission was significantly enhanced in the presence of protons or Cu 2 + due to transformation from the pirocyclic form to open-ring form. The fluorescence was quenched sequentially upon irradiation with 297 nm light based on the intramolecular fluorescence resonance energy transfer mechanism. In an acetonitrile: water binary solvent (1: 1 v/v), the compound showed significant fluorescent enhancement for Cu 2 + compared with a wide range of tested metal ions with a fast response and a limit of detection of 2.86 × 10 -8  mol L -1 . Using Cu 2 + and UV light as the chemical inputs, and fluorescence intensity at 597 nm as the output, a logic gate was developed at the molecular level. Moreover, the compound can be used with a high accuracy to detect Cu 2 + in a natural water sample. Copyright © 2016 John Wiley & Sons, Ltd.

  2. Rhodamine B-conjugated encrypted vipericidin nonapeptide is a potent toxin to zebrafish and associated with in vitro cytotoxicity.

    Science.gov (United States)

    Wang, Liang; Chan, Judy Y W; Rêgo, Juciane V; Chong, Cheong-Meng; Ai, Nana; Falcão, Cláudio B; Rádis-Baptista, Gandhi; Lee, Simon M Y

    2015-06-01

    Animal venoms contain a diverse array of proteins and enzymes that are toxic toward various physiological systems. However, there are also some practical medicinal uses for these toxins including use as anti-bacterial and anti-tumor agents. In this study, we identified a nine-residue cryptic oligopeptide, KRFKKFFKK (EVP50) that is repeatedly encoded in tandem within vipericidin sequences. EVP50 displayed in vivo potent lethal toxicity to zebrafish larvae (LD50=6 μM) when the peptide's N-terminus was chemically conjugated to rhodamine B (RhoB). In vitro, RhoB-conjugated EVP50 (RhoB-EVP50) exhibited a concentration-dependent cytotoxic effect toward MCF-7 and MDA-MB-231 breast cancer cells. In MCF-7 cells, the RhoB-EVP50 nonapeptide accumulated inside the cells within minutes. In the cytoplasm, the RhoB-EVP50 induced extracellular calcium influx and intracellular calcium release. Membrane budding was also observed after incubation with micromolar concentrations of the fluorescent EVP50 conjugate. The conjugate's interference with calcium homeostasis, its intracellular accumulation and its induced membrane dysfunction (budding and vacuolization) seem to act in concert to disrupt the cell circuitry. Contrastively, unconjugated EVP50 peptide did not display neither toxic nor cytotoxic activities in our in vivo and in vitro models. The synergic mechanism of toxicity was restricted to the structurally modified encrypted vipericidin nonapeptide. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Thiophene-based rhodamine as selectivef luorescence probe for Fe(III) and Al(III) in living cells.

    Science.gov (United States)

    Wang, Kun-Peng; Chen, Ju-Peng; Zhang, Si-Jie; Lei, Yang; Zhong, Hua; Chen, Shaojin; Zhou, Xin-Hong; Hu, Zhi-Qiang

    2017-09-01

    The thiophene-modified rhodamine 6G (GYJ) has been synthesized as a novel chemosensor. The sensor has sufficiently high selectivity and sensitivity for the detection of Fe 3+ and Al 3+ ions (M 3+ ) by fluorescence and ultraviolet spectroscopy with a strong ability for anti-interference performance. The binding ratio of M 3+ -GYJ complex was determined to be 2:1 according to the Job's plot. The binding constants for Fe 3+ and Al 3+ were calculated to be 3.91 × 10 8 and 5.26 × 10 8  M -2 , respectively. All these unique features made it particularly favorable for cellular imaging applications. The obvious fluorescence microscopy experiments demonstrated that the probes could contribute to the detection of Fe 3+ and Al 3+ in related cells and biological organs with satisfying resolution. Graphical abstract GYJ has high selectivity and sensitivity for the detection of Fe(III) and Al(III) with the binding ratio of 2:1.

  4. Preparation and Utilization of Kapok Hull Carbon for the Removal of Rhodamine-B from Aqueous Solution

    Directory of Open Access Journals (Sweden)

    P. S. Syed Shabudeen

    2006-01-01

    Full Text Available A carbonaceous sorbent prepared from the indegeneous agricultural waste (which is facing solid waste disposal problem Kapok Hull, by acid treatment was tested for its efficiency in removing basic dyes. Batch kinetic and isotherm experiments were conducted to determine the sorption and desorption of the Rhodamine-B from aqueous solution with activated carbon. The factors affecting the rate processes involved in the removal of dye for initial dye concentration, agitation time, and carbon dose and particle size have been studied at ambient temperature. The adsorption process followed first order rate kinetics. The first-order rate equation by Lagergren was tested on the kinetic data, and isotherm data was analyzed for possible agreement with the Langmuir and Freundlich adsorption isotherm equations. The intraparticle diffusion rate equation from which adsorption rate constants, diffusion rate constants and diffusion coefficients were determined. Intraparticle diffusion was found to be the rate-limiting step. The structural and morphological of activated carbon were characterized by XRD and SEM studies respectively.

  5. Photoinduced intermolecular electron transfer and off-resonance Raman characteristics of Rhodamine 101/N,N-diethylaniline

    International Nuclear Information System (INIS)

    Jiang, Li-lin; Liu, Wei-long; Song, Yun-fei; He, Xing; Wang, Yang; Wang, Chang; Wu, Hong-lin; Yang, Fang; Yang, Yan-qiang

    2014-01-01

    Highlights: • Mechanism of PIET reaction process for the Rh101 + /DEA system is investigated. • The significant geometrical changes of the charge–transfer complex are explained. • Forward Electron transfer from DEA to Rh101 +∗ occurs with lifetime of 425–560 fs. • Backward electron transfer occurs with a time constant of 46.16–51.40 ps. • Intramolecular vibrational relaxation occurs with lifetime of 2.77–5.39 ps. - Abstract: The ultrafast photoinduced intermolecular electron transfer (PIET) reaction of Rhodamine 101 (Rh101 + ) in N,N-diethylaniline (DEA) was investigated using off-resonance Raman, femtosecond time-resolved multiplex transient grating (TG) and transient absorption (TA) spectroscopies. The Raman spectra indicate that the C=C stretching vibration of the chromophore aromatic ring is more sensitive to ET compared with the C-C stretching mode. The ultrafast photoinduced intermolecular forward ET (FET) from DEA to Rh101 +∗ occurs on a time scale of τ FET = 425–560 fs. The backward ET (BET) occurs in the inverted region with a time constant of τ BET = 46.16–51.40 ps. The intramolecular vibrational relaxation (IVR) process occurs on the excited state potential energy surface with the time constant of τ IVR = 2.77–5.39 ps

  6. Enhanced photosensitized degradation of rhodamine B on CdS/TiO{sub 2} nanocomposites under visible light irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Li, Wenjuan [Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Sciences and Engineering, Nanjing University of Information Sciences and Engineering, Nanjing 210044 (China); Research Institute of Photocatalysis, State Key Laboratory Breeding Base of Photocatalysis, Fuzhou University, Fuzhou 350002 (China); Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu 273165 (China); Cui, Xiaoli [Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu 273165 (China); Wang, Peixian; Shao, Yu [Research Institute of Photocatalysis, State Key Laboratory Breeding Base of Photocatalysis, Fuzhou University, Fuzhou 350002 (China); Li, Danzhen, E-mail: dzli@fzu.edu.cn [Research Institute of Photocatalysis, State Key Laboratory Breeding Base of Photocatalysis, Fuzhou University, Fuzhou 350002 (China); Teng, Fei [Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Sciences and Engineering, Nanjing University of Information Sciences and Engineering, Nanjing 210044 (China)

    2013-09-01

    Graphical abstract: The photosensitized degradation of RhB was largely enhanced by the synergistic effect of the RhB and CdS/TiO{sub 2} nanocomposite. The composite of the two inorganic semiconductors with appropriate oxidation reduction energy levels enhanced the charge separation and extended the absorption response to visible region. - Highlights: • CdS/TiO{sub 2} nanocomposites were synthesized by a simple hydrothermal method. • Samples prepared at 200 °C, 12 h, CdS/TiO{sub 2} = 12% possessed the best activity. • The photosensitized degradation of RhB was largely enhanced by the composite. • The better activity was due to the synergistic effect of the RhB and CdS/TiO{sub 2}. - Abstract: Visible-light-driven photocatalysts, CdS/TiO{sub 2} nanocomposites were synthesized by a simple hydrothermal method. Their formation and structures were characterized by X-ray diffractometer, transmission electron microscopy, diffuse reflectance spectroscopy, and X-ray photoelectron spectroscopy. Taking rhodamine B (RhB) as a model, their photocatalytic activities in aqueous phase under visible light irradiation (420 < λ < 800 nm) were tested. The results showed that the composite of CdS and TiO{sub 2} with appropriate oxidation reduction energy levels enhanced the charge separation and extended the absorption response into visible light region. Thus, the photosensitized degradation of RhB was largely enhanced. The degradation mechanism was explored concretely.

  7. Electronic parameters of high barrier Au/Rhodamine-101/n-Inp Schottky diode with organic ınterlayer

    International Nuclear Information System (INIS)

    Güllü, Ö.; Aydoğan, S.; Türüt, A.

    2012-01-01

    In this work, we present that Rhodamine-101 (Rh-101) organic molecules can control the electrical characteristics of conventional Au/n-InP metal–semiconductor contacts. An Au/n-InP Schottky junction with Rh-101 interlayer has been formed by using a simple cast process. A potential barrier height as high as 0.88 eV has been achieved for Au/Rh-101/n-InP Schottky diodes, which have good current–voltage (I–V) characteristics. This good performance is attributed to the effect of formation of interfacial organic thin layer between Au and n-InP. By using capacitance-voltage measurement of the Au/Rh-101/n-InP Schottky diode the diffusion potential and the barrier height have been calculated as 0.78 V and 0.88 eV, respectively. From the I–V measurement of the diode under illumination, short circuit current and open circuit voltage have been extracted as 1.70 μA and 240 mV, respectively.

  8. Dual-signal fenamithion probe by combining fluorescence with colorimetry based on Rhodamine B modified silver nanoparticles.

    Science.gov (United States)

    Cui, Zhimin; Han, Cuiping; Li, Haibing

    2011-04-07

    A versatile yet simple strategy for the fabrication of a highly selective and sensitive fenamithion probe based on Rhodamine B (RB) modified silver nanoparticles (RB-Ag NPs) was developed. The advantage of our system over classical assays is that it combined fluorescence with colorimetry which can realize the prompt on-site and real-time detection of fenamithion with high sensitivity (0.1 nM) in aqueous solution. Moreover, the detection system presents excellent anti-disturbance ability when exposed to a series of interfering ionic/pesticides mixtures and can be applied to the determination of fenamithion in real vegetables and different water samples with the limit of detection (LOD) as low as 10 nM (0.0026 mg L(-1)), which is in accord with the maximum contamination level of 0.001∼0.25 mg L(-1) for organophosphorus pesticides as defined by the U.S. Environmental Protection Agency (EPA). Advantage is taken of the fact that RB would be displaced from the surface of the Ag NPs because of the stronger coordination ability of Ag NPs with fenamithion, an amino-containing organophosphorus pesticide, accompanying the clustered Ag NPs (9 nm) dissipating into smaller individual particles (7 nm). Based on this phenomenon, a novel analyte-induced etching mechanism was proposed. © The Royal Society of Chemistry 2011

  9. Two rhodamine lactam modulated lysosome-targetable fluorescence probes for sensitively and selectively monitoring subcellular organelle pH change

    Energy Technology Data Exchange (ETDEWEB)

    Li, Hongmei [Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi' an 710069 (China); Wang, Cuiling [Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi' an 710069 (China); She, Mengyao; Zhu, Yuelu; Zhang, Jidong; Yang, Zheng [Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi' an 710069 (China); Liu, Ping, E-mail: liuping@nwu.edu.cn [Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi' an 710069 (China); Wang, Yaoyu [Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi' an 710069 (China); Li, Jianli, E-mail: lijianli@nwu.edu.cn [Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi' an 710069 (China)

    2015-11-05

    Be a powerful technique for convenient detection of pH change in living cells, especially at subcellular level, fluorescent probes has attracted more and more attention. In this work, we designed and synthesized three rhodamine lactam modulated fluorescent probes RS1, RS2 and RS3, which all respond sensitively toward weak acidity (pH range 4–6) via the photophysical property in buffer solution without interference from the other metal ions, and they also show ideal pKa values and excellent reversibility. Particularly, by changing the lone pair electrons distribution of lactam-N atom with different conjugations, RS2 and RS3 exhibit high quantum yield, negligible cytotoxicity and excellent permeability. They are suitable to stain selectively lysosomes of tumor cells and monitor its pH changes sensitively via optical molecular imaging. The above findings suggest that the probes we designed could act as ideal and easy method for investigating the pivotal role of H{sup +} in lysosomes and are potential pH detectors in disease diagnosis through direct intracellular imaging. - Highlights: • Two probes for sensitively and selectively monitoring weak acidic pH change. • The pKa of the probes was highly suitable for staining lysosomes in tumor cells. • The properties of those probes were changed by different conjugate system. • These probes have negligible cytotoxicity and good sensitivity in vivo.

  10. Graphene oxide based CdSe photocatalysts: Synthesis, characterization and comparative photocatalytic efficiency of rhodamine B and industrial dye

    International Nuclear Information System (INIS)

    Ghosh, Trisha; Lee, Jeong-Ho; Meng, Ze-Da; Ullah, Kefayat; Park, Chong-Yeon; Nikam, Vikram; Oh, Won-Chun

    2013-01-01

    Highlights: ► CdSe–graphene is synthesized by hydrothermal method. ► Three molar solutions of CdSe were used making three different composites. ► RhB and Texbrite MST-L were used as sample dye solutions. ► Texbrite MST-L is photo degraded in visible light. ► UV-spectroscopic analysis was done to measure degradation. - Abstract: CdSe–graphene composites were prepared using simple “hydrothermal method” where the graphene surface was modified using different molar solutions of cadmium selenide (CdSe) in aqueous media. The characterization of CdSe–graphene composites were studied by X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscope (SEM), and with transmission electron microscope (TEM). The catalytic activities of CdSe-composites were evaluated by degradation of rhodamine B (RhB) and commercial industrial dye “Texbrite MST-L (TXT-MST)” with fixed concentration. The degradation was observed by the decrease in the absorbance peak studied by UV spectrophotometer. The decrease in the dye concentration indicated catalytic degradation effect by CdSe–graphene composites

  11. Kinetics, isothermal and thermodynamics studies of electrocoagulation removal of basic dye rhodamine B from aqueous solution using steel electrodes

    Science.gov (United States)

    Adeogun, Abideen Idowu; Balakrishnan, Ramesh Babu

    2017-07-01

    Electrocoagulation was used for the removal of basic dye rhodamine B from aqueous solution, and the process was carried out in a batch electrochemical cell with steel electrodes in monopolar connection. The effects of some important parameters such as current density, pH, temperature and initial dye concentration, on the process, were investigated. Equilibrium was attained after 10 min at 30 °C. Pseudo-first-order, pseudo-second-order, Elovich and Avrami kinetic models were used to test the experimental data in order to elucidate the kinetic adsorption process; pseudo-first-order and Avrami models best fitted the data. Experimental data were analysed using six model equations: Langmuir, Freudlinch, Redlich-Peterson, Temkin, Dubinin-Radushkevich and Sips isotherms and it was found that the data fitted well with Sips isotherm model. The study showed that the process depends on current density, temperature, pH and initial dye concentration. The calculated thermodynamics parameters (Δ G°, Δ H° and Δ S°) indicated that the process is spontaneous and endothermic in nature.

  12. Amorphous TiO2 doped with carbon for visible light photodegradation of rhodamine B and 4-chlorophenol

    International Nuclear Information System (INIS)

    Shao, Penghui; Tian, Jiayu; Zhao, Zhiwei; Shi, Wenxin; Gao, Shanshan; Cui, Fuyi

    2015-01-01

    Graphical abstract: - Highlights: • Amorphous TiO 2 doped with carbon is prepared as a visible photocatalyst. • RhB and 4-chlorophenol are decomposed effectively by carbon-doped amorphous TiO 2 . • The mechanism for visible light photocatalysis is discussed detailedly. - Abstract: Visible light photocatalytic activity of amorphous TiO 2 doped with carbon is prepared by a facile sol-gel route for the first time. The most active sample with mesostructure of amorphous phase, high surface area (273 m 2 g −1 ) and large pore volume (0.33 cm 3 g −1 ) is identified by X-ray diffractometer, Raman spectrometer, transmission electron microscope and N 2 adsorption–desorption isotherms. In addition, the most active sample is characterized by Fourier transform-infrared spectrometer, X-ray photoelectron spectrometer, UV–vis diffuse reflectance spectrometer and luminescence spectrometer. The results show that the most active sample with oxygenic groups has a narrower bandgap and lower recombination of electron–hole, due to the carbon doping and phase of amorphous. Effective photodegradation capability and stability of rhodamine B and colorless 4-chlorophenol are verified by photocatalytic tests under visible light irradiation. A possible mechanism of amorphous TiO 2 doped with carbon for visible light photocatalysis is proposed. The findings of this paper will provide new insights to design visible light-induced photocatalyst based on amorphous TiO 2 for organic removal

  13. Photoinduced intermolecular electron transfer and off-resonance Raman characteristics of Rhodamine 101/N,N-diethylaniline

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Li-lin [Department of Physics, Harbin Institute of Technology, Harbin 150001 (China); School of Mechanical and Electronic Engineering, Hezhou University, Hezhou 542800 (China); Liu, Wei-long; Song, Yun-fei; He, Xing; Wang, Yang; Wang, Chang; Wu, Hong-lin [Department of Physics, Harbin Institute of Technology, Harbin 150001 (China); Yang, Fang [National Key Laboratory of Science and Technology on Tunable Laser, Department of Optoelectronics Information Science Technology, Harbin Institute of Technology, Harbin 150001 (China); Yang, Yan-qiang, E-mail: yqyang@hit.edu.cn [Department of Physics, Harbin Institute of Technology, Harbin 150001 (China); National Key Laboratory of Shock Wave and Detonation Physics, Institute of Fluid Physics, China Academy of Engineering Physics, Mianyang 621900, Sichuan (China)

    2014-01-31

    Highlights: • Mechanism of PIET reaction process for the Rh101{sup +}/DEA system is investigated. • The significant geometrical changes of the charge–transfer complex are explained. • Forward Electron transfer from DEA to Rh101{sup +∗} occurs with lifetime of 425–560 fs. • Backward electron transfer occurs with a time constant of 46.16–51.40 ps. • Intramolecular vibrational relaxation occurs with lifetime of 2.77–5.39 ps. - Abstract: The ultrafast photoinduced intermolecular electron transfer (PIET) reaction of Rhodamine 101 (Rh101{sup +}) in N,N-diethylaniline (DEA) was investigated using off-resonance Raman, femtosecond time-resolved multiplex transient grating (TG) and transient absorption (TA) spectroscopies. The Raman spectra indicate that the C=C stretching vibration of the chromophore aromatic ring is more sensitive to ET compared with the C-C stretching mode. The ultrafast photoinduced intermolecular forward ET (FET) from DEA to Rh101{sup +∗} occurs on a time scale of τ{sub FET} = 425–560 fs. The backward ET (BET) occurs in the inverted region with a time constant of τ{sub BET} = 46.16–51.40 ps. The intramolecular vibrational relaxation (IVR) process occurs on the excited state potential energy surface with the time constant of τ{sub IVR} = 2.77–5.39 ps.

  14. UV-visible spectroscopic estimation of photodegradation of rhodamine-B dye using tin(IV) oxide nanoparticles.

    Science.gov (United States)

    Sangami, G; Dharmaraj, N

    2012-11-01

    Nanocrystalline, tin(IV) oxide (SnO(2)) particles has been prepared by thermal decomposition of tin oxalate precursor obtained from the reactions of tin(IV) chloride and sodium oxalate using eggshell membrane (ESM). The as-prepared SnO(2) nanoparticles were characterized by thermal studies, transmission electron microscopy (TEM), powder X-ray diffraction (XRD), Raman, FT-IR and UV-visible studies and used as a photocatalyst for the degradation of rhodamine-B (Rh-B) dye. The size of the prepared nanoparticles was in the range of 5-12nm as identified from the TEM images. Powder XRD data revealed the presence of a tetragonal, rutile crystalline phase of the tin(IV) oxide nanoparticles. Thermal analysis showed that the decomposition of tin oxalate precursor to yield the titled tin(IV) oxide nanoparticles was completed below 500°C. The extent of degradation of Rh-B in the presence of SnO(2) monitored by absorption spectral measurements demonstrated that 94.48% of the selected dye was degraded upon irradiation with UV light for 60 min. Copyright © 2012 Elsevier B.V. All rights reserved.

  15. Separation of toxic rhodamine B from aqueous solution using an efficient low-cost material, Azolla pinnata, by adsorption method.

    Science.gov (United States)

    Kooh, Muhammad Raziq Rahimi; Lim, Linda B L; Lim, Lee Hoon; Dahri, Muhammad Khairud

    2016-02-01

    This study investigated the potential of untreated Azolla pinnata (AP) to remove toxic rhodamine B (RB) dye. The effects of adsorbent dosage, pH, ionic strength, contact time, and concentration were studied. Experiments involving the effects of pH and ionic strength indicated that hydrophobic-hydrophobic interactions might be the dominant force of attraction for the RB-AP adsorption system. The kinetics modelling of the kinetics experiment showed that pseudo-second-order best represented the adsorption process. The Weber-Morris intraparticle diffusion model showed that intraparticle diffusion is not the rate-limiting step, while the Boyd model suggested that film diffusion might be rate-limiting. The adsorption isotherm model, Langmuir, best represented the adsorption process, and the maximum adsorption capacity was predicted to be 72.2 and 199.7 mg g(-1) at 25 and 65 °C, respectively. Thermodynamics study indicates spontaneity, endothermic and physisorption-dominant adsorption process. The adsorbents were regenerated to satisfactory level with distilled water, HNO3 and NaOH. Pre-treatment of adsorbent with oxalic acid, citric acid, NaOH, HCl and phosphoric acid was investigated but the adsorption capacity was less than the untreated AP.

  16. Two rhodamine lactam modulated lysosome-targetable fluorescence probes for sensitively and selectively monitoring subcellular organelle pH change

    International Nuclear Information System (INIS)

    Li, Hongmei; Wang, Cuiling; She, Mengyao; Zhu, Yuelu; Zhang, Jidong; Yang, Zheng; Liu, Ping; Wang, Yaoyu; Li, Jianli

    2015-01-01

    Be a powerful technique for convenient detection of pH change in living cells, especially at subcellular level, fluorescent probes has attracted more and more attention. In this work, we designed and synthesized three rhodamine lactam modulated fluorescent probes RS1, RS2 and RS3, which all respond sensitively toward weak acidity (pH range 4–6) via the photophysical property in buffer solution without interference from the other metal ions, and they also show ideal pKa values and excellent reversibility. Particularly, by changing the lone pair electrons distribution of lactam-N atom with different conjugations, RS2 and RS3 exhibit high quantum yield, negligible cytotoxicity and excellent permeability. They are suitable to stain selectively lysosomes of tumor cells and monitor its pH changes sensitively via optical molecular imaging. The above findings suggest that the probes we designed could act as ideal and easy method for investigating the pivotal role of H + in lysosomes and are potential pH detectors in disease diagnosis through direct intracellular imaging. - Highlights: • Two probes for sensitively and selectively monitoring weak acidic pH change. • The pKa of the probes was highly suitable for staining lysosomes in tumor cells. • The properties of those probes were changed by different conjugate system. • These probes have negligible cytotoxicity and good sensitivity in vivo.

  17. Upconversion NaYF4 Nanoparticles for Size Dependent Cell Imaging and Concentration Dependent Detection of Rhodamine B

    Directory of Open Access Journals (Sweden)

    Shigang Hu

    2015-01-01

    Full Text Available Upconversion nanoparticles (UCNPs based on NaYF4 nanocrystals with strong upconversion luminescence are synthesized by the solvothermal method. The emission color of these NaYF4 upconversion nanoparticles can be easily modulated by the doping. These NaYF4 upconversion nanocrystals can be employed as fluorescence donors to pump fluorescent organic molecules. For example, the efficient luminescence resonant energy transfer (LRET can be achieved by controlling the distance between NaYF4:Yb3+/Er3+ UCNPs and Rhodamine B (RB. NaYF4:Yb3+/Er3+ UCNPs can emit green light at the wavelength of ~540 nm while RB can efficiently absorb the green light of ~540 nm to emit red light of 610 nm. The LRET efficiency is highly dependent on the concentration of NaYF4 upconversion fluorescent donors. For the fixed concentration of 3.2 µg/mL RB, the optimal concentration of NaYF4:Yb3+/Er3+ UCNPs is equal to 4 mg/mL which generates the highest LRET signal ratio. In addition, it is addressed that the upconversion nanoparticles with diameter of 200 nm are suitable for imaging the cells larger than 10 µm with clear differentiation between cell walls and cytoplasm.

  18. UV or X-irradiation increases the cytoplasmic accumulation of rhodamine 123 in various cancer cell lines

    International Nuclear Information System (INIS)

    Dumitriu, I.E.; Beyer, T.D.; Gaipl, U.S.; Kalden, J.R.; Herrmann, M.; Roedel, F.

    2003-01-01

    Purpose: Previous studies indicated that ATP-binding cassette (ABC) membrane transporters protect against UV-induced apoptosis. We investigated the effect of UVB and X-ray irradiation on the export function of these ABC transporters in primary lymphocytes and various cancer cell lines. Material and Methods: We used rhodamine accumulation assays in various human malignant cell lines and peripheral blood lymphocytes (PBL). Cells were irradiated with up to 960 mJ/cm 2 and up to 50 Gy of UVB and X-ray, respectively. Results: We demonstrated that UVB as well as X-ray irradiation inhibit the export function of the ABC transporters in a dose-dependent fashion. For PBL, this effect did not correlate with an apoptotic phenotype. In the case of the tumor cell lines, even though the irradiation-induced inhibition of membrane transporters was accompanied by phosphatidylserine exposure, only a minority of cells had lost their mitochondrial membrane potential during the observation period. Furthermore, we demonstrated that the inhibition of membrane transporters is not a general feature of apoptosis. Conclusion: Irradiation inhibits the export function of ABC transporters. Although some of the irradiated cells undergo apoptosis following irradiation, the inhibition is an unique feature accompanying irradiation and not a general hallmark of apoptotic cell death. The inhibition of drug export by irradiation may offer new potential for reverting multidrug resistance of cancer cells. (orig.)

  19. UV or X-irradiation increases the cytoplasmic accumulation of rhodamine 123 in various cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Dumitriu, I.E.; Beyer, T.D.; Gaipl, U.S.; Kalden, J.R.; Herrmann, M. [Inst. for Clinical Immunology, Dept. of Medicine III, Friedrich Alexander Univ. of Erlangen-Nuremberg, Erlangen (Germany); Roedel, F. [Dept. of Radiooncology, Univ. of Erlangen-Nuremberg, Erlangen (Germany)

    2003-08-01

    Purpose: Previous studies indicated that ATP-binding cassette (ABC) membrane transporters protect against UV-induced apoptosis. We investigated the effect of UVB and X-ray irradiation on the export function of these ABC transporters in primary lymphocytes and various cancer cell lines. Material and Methods: We used rhodamine accumulation assays in various human malignant cell lines and peripheral blood lymphocytes (PBL). Cells were irradiated with up to 960 mJ/cm{sup 2} and up to 50 Gy of UVB and X-ray, respectively. Results: We demonstrated that UVB as well as X-ray irradiation inhibit the export function of the ABC transporters in a dose-dependent fashion. For PBL, this effect did not correlate with an apoptotic phenotype. In the case of the tumor cell lines, even though the irradiation-induced inhibition of membrane transporters was accompanied by phosphatidylserine exposure, only a minority of cells had lost their mitochondrial membrane potential during the observation period. Furthermore, we demonstrated that the inhibition of membrane transporters is not a general feature of apoptosis. Conclusion: Irradiation inhibits the export function of ABC transporters. Although some of the irradiated cells undergo apoptosis following irradiation, the inhibition is an unique feature accompanying irradiation and not a general hallmark of apoptotic cell death. The inhibition of drug export by irradiation may offer new potential for reverting multidrug resistance of cancer cells. (orig.)

  20. Application of AlMCM-41 for competitive adsorption of methylene blue and rhodamine B: Thermodynamic and kinetic studies

    International Nuclear Information System (INIS)

    Eftekhari, S.; Habibi-Yangjeh, A.; Sohrabnezhad, Sh.

    2010-01-01

    AlMCM-41 was applied for adsorption of methylene blue (MB) and rhodamine B (RB) in single and binary component systems. In the single component systems, AlMCM-41 represents higher adsorption capacity for MB than RB with the maximal adsorption capacity of 2.08 x 10 -4 and 8.74 x 10 -5 mol/g at 25 deg. C for MB and RB, respectively. In the binary component system, MB and RB exhibit competitive adsorption onto the adsorbent. The adsorption is approximately reduced to 94 and 79% of single component adsorption systems for MB and RB (initial concentration of 8 x 10 -6 M) at 25 deg. C. In single and binary component systems, kinetic and adsorption isotherm studies demonstrate that the data are following pseudo-second-order kinetic model and Langmuir isotherm. Effect of solution pH on the adsorption in single and binary component systems was studied and the results were described by electrostatic interactions.

  1. Flotation-spectrophotometric determination of ruthenium in the Ru(IV)-chloride-rhodamine 6G-toluene system

    International Nuclear Information System (INIS)

    Balcerzak, M.

    1985-01-01

    The reduction of RuO 4 in hydrochloric acid has been examined. A sensitive flotation-spectrophotometric method of the determination of ruthenium based on the ion associate formed by the anionic chlorid complex of ruthenium RuCl 2- 6 with the basic dye Rhodamine 6G (R6G) has been developed. The solution of the ion associate obeys Beer's law up to the concentration of 0.25 μg Ru/ml. The ion associate precipitates when the aqueous solution is shaken with toluene. The separated compound is dissolved in acetone. The molar absorptivity (epsilon) at 530 nm is 5.1 x 10 5 l x mole -1 x cm -1 . The relative standard deviation is 3-7%. The mole ratio of Ru:R6G in the complex is 1:5. Osmium reacts similarly. The determination of ruthenium can be selective after the preliminary separation of osmium as OsO 4 . The method was applied to the determination of microgram amounts of ruthenium in crucible platinum. (Author)

  2. A six-membered-ring incorporated Si-rhodamine for imaging of copper(ii) in lysosomes.

    Science.gov (United States)

    Wang, Baogang; Cui, Xiaoyan; Zhang, Zhiqiang; Chai, Xiaoyun; Ding, Hao; Wu, Qiuye; Guo, Zhongwu; Wang, Ting

    2016-07-12

    The regulation of copper homeostasis in lysosomes of living cells is closely related to various physiological and pathological processes. Thus, it is of urgent need to develop a fluorescent probe for selectively and sensitively monitoring the location and concentration of lysosomal Cu(2+). Herein, a six-membered ring, thiosemicarbazide, was incorporated into a Si-rhodamine (SiR) scaffold for the first time, affording a SiR-based fluorescent probe SiRB-Cu. Through the effective Cu(2+)-triggered ring-opening process, the probe exhibits fast NIR chromogenic and fluorogenic responses to Cu(2+) within 2 min as the result of formation of a highly fluorescent product SiR-NCS. Compared with a five-membered ring, the expanded ring retains great tolerance to H(+), ensuring the superior sensitivity with a detection limit as low as 7.7 nM and 200-fold enhancement of relative fluorescence in the presence of 1.0 equiv. of Cu(2+) in pH = 5.0 solution, the physiological pH of lysosome. Moreover, the thiosemicarbazide moiety acts not only as the chelating and reactive site, but also as an efficient lysosome-targeting group, leading to the proactive accumulation of the probe into lysosomes. Taking advantage of these distinct properties, SiRB-Cu provides a functional probe suitable for imaging exogenous and endogenous lysosomal Cu(2+) with high imaging contrast and fidelity.

  3. LiFePO4 microcrystals as an efficient heterogeneous Fenton-like catalyst in degradation of rhodamine 6G.

    Science.gov (United States)

    Li, Zhan Jun; Ali, Ghafar; Kim, Hyun Jin; Yoo, Seong Ho; Cho, Sung Oh

    2014-01-01

    We present a novel heterogeneous Fenton-like catalyst of LiFePO4 (LFP). LFP has been widely used as an electrode material of a lithium ion battery, but we observed that commercial LFP (LFP-C) could act as a good Fenton-like catalyst to decompose rhodamine 6G. The catalytic activity of LFP-C microparticles was much higher than a popular catalyst, magnetite nanoparticles. Furthermore, we found that the catalytic activity of LFP-C could be further increased by increasing the specific surface area. The reaction rate constant of the hydrothermally synthesized LFP microcrystals (LFP-H) is at least 18 times higher than that of magnetite nanoparticles even though the particle size of LFP is far larger than magnetite nanoparticles. The LFP catalysts also exhibited a good recycling behavior and high stability under an oxidizing environment. The effects of the experimental parameters such as the concentration of the catalysts, pH, and the concentration of hydrogen peroxide on the catalytic activity of LFP were also analyzed.

  4. Highly Sensitive Fluorescent Sensor for Cartap Based on Fluorescence Resonance Energy Transfer Between Gold Nanoparticles and Rhodamine B.

    Science.gov (United States)

    Dong, Liang; Hou, Changjun; Fa, Huanbao; Yang, Mei; Wu, Huixiang; Zhang, Liang; Huo, Danqun

    2018-04-01

    Cartap residue poses a great threat to human health and its derivatives would remain in soils, natural waters and other environmental domains for a long time. Herein, a simple, rapid and ultrasensitive analytical method for the determination of cartap based on fluorescence resonance energy transfer (FRET) between Au nanoparticles (AuNPs) and rhodamine B (RB) is first described. With the presence of citrate-stabilized AuNPs, the fluorescence of RB was remarkably quenched by AuNPs via FRET. The fluorescence of the AuNPs-RB system was recovered upon addition of cartap, cartap can be adsorbed on the surface of AuNPs due to its amino group that has good affinity with gold, which could induce the aggregation of AuNPs accompanying color change from red to blue. Thus, the FRET between AuNPs and RB was weakened and the PL intensity of RB was recovered accordingly. A good linear correlation for detection of RB was exhibited from 1 nM to 180 nM, and the detection limit reached 0.88 nM, which was much lower than the safety limit required by USA, UK and China. To the best of our knowledge, it has been the lowest detection ever without the aid of costly instrumentation. This method was successfully carried out for the assessment of cartap in real samples with satisfactory results, which revealed many advantages such as high sensitivity, low cost and non-time-consuming compared with traditional methods.

  5. Enhanced photocatalytic performance and degradation pathway of Rhodamine B over hierarchical double-shelled zinc nickel oxide hollow sphere heterojunction

    Science.gov (United States)

    Zhang, Ying; Zhou, Jiabin; Cai, Weiquan; Zhou, Jun; Li, Zhen

    2018-02-01

    In this study, hierarchical double-shelled NiO/ZnO hollow spheres heterojunction were prepared by calcination of the metallic organic frameworks (MOFs) as a sacrificial template in air via a one-step solvothermal method. Additionally, the photocatalytic activity of the as-prepared samples for the degradation of Rhodamine B (RhB) under UV-vis light irradiation were also investigated. NiO/ZnO microsphere comprised a core and a shell with unique hierarchically porous structure. The photocatalytic results showed that NiO/ZnO hollow spheres exhibited excellent catalytic activity for RhB degradation, causing complete decomposition of RhB (200 mL of 10 g/L) under UV-vis light irradiation within 3 h. Furthermore, the degradation pathway was proposed on the basis of the intermediates during the photodegradation process using liquid chromatography analysis coupled with mass spectroscopy (LC-MS). The improvement in photocatalytic performance could be attributed to the p-n heterojunction in the NiO/ZnO hollow spheres with hierarchically porous structure and the strong double-shell binding interaction, which enhances adsorption of the dye molecules on the catalyst surface and facilitates the electron/hole transfer within the framework. The degradation mechanism of pollutant is ascribed to the hydroxyl radicals (rad OH), which is the main oxidative species for the photocatalytic degradation of RhB. This work provides a facile and effective approach for the fabrication of porous metal oxides heterojunction with high photocatalytic activity and thus can be potentially used in the environmental purification.

  6. Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells.

    Science.gov (United States)

    Cmiel, Vratislav; Skopalik, Josef; Polakova, Katerina; Solar, Jan; Havrdova, Marketa; Milde, David; Justan, Ivan; Magro, Massimiliano; Starcuk, Zenon; Provaznik, Ivo

    2017-07-01

    In the last few years, magnetically labeled cells have been intensively explored, and non-invasive cell tracking and magnetic manipulation methods have been tested in preclinical studies focused on cell transplantation. For clinical applications, it is desirable to know the intracellular pathway of nanoparticles, which can predict their biocompatibility with cells and the long-term imaging properties of labeled cells. Here, we quantified labeling efficiency, localization, and fluorescence properties of Rhodamine derivatized superparamagnetic maghemite nanoparticles (SAMN-R) in mesenchymal stromal cells (MSC). We investigated the stability of SAMN-R in the intracellular space during a long culture (20 days). Analyses were based on advanced confocal microscopy accompanied by atomic absorption spectroscopy (AAS) and magnetic resonance imaging. SAMN-R displayed excellent cellular uptake (24 h of labeling), and no toxicity of SAMN-R labeling was found. 83% of SAMN-R nanoparticles were localized in lysosomes, only 4.8% were found in mitochondria, and no particles were localized in the nucleus. On the basis of the MSC fluorescence measurement every 6 days, we also quantified the continual decrease of SAMN-R fluorescence in the average single MSC during 18 days. An additional set of analyses showed that the intracellular SAMN-R signal decrease was minimally caused by fluorophore degradation or nanoparticles extraction from the cells, main reason is a cell division. The fluorescence of SAMN-R nanoparticles within the cells was detectable minimally for 20 days. These observations indicate that SAMN-R nanoparticles have a potential for application in transplantation medicine.

  7. Rhodamine B in dissolved and nano-bound forms: Indicators for light-based advanced oxidation processes.

    Science.gov (United States)

    Shabat-Hadas, Efrat; Mamane, Hadas; Gitis, Vitaly

    2017-10-01

    Rhodamine B (RhB) is a water-soluble fluorescent dye that is often used to determine flux and flow direction in biotechnological and environmental applications. In the current research, RhB in soluble (termed free) and virus-bound (termed nano-bound) forms was used as an efficiency indicator for three environmental processes. The degradation of free and nano-bound RhB by (i) direct UV photolysis and (ii) UV/H 2 O 2 advanced oxidation process (AOP) was studied in a collimated beam apparatus equipped with medium-pressure mercury vapor lamp. The degradation by (iii) solar light-induced photocatalysis was studied in a solar simulator with titanium dioxide and bismuth photocatalysts. Results showed negligible RhB degradation by direct UV and solar light, and its nearly linear degradation by UV/H 2 O 2 and photocatalysis/photosensitization in the presence of a solid catalyst. Considerable adsorption of free RhB on bismuth-based catalyst vs. no adsorption of nano-bound RhB on this catalyst or of any form of the dye on titanium dioxide produced two important conclusions. First, the better degradation of free RhB by the bismuth catalyst suggests that close proximity of a catalyst hole and the decomposing molecule significantly influences degradation. Second, the soluble form of the dye might not be the best option for its use as an indicator. Nano-bound RhB showed high potential as an AOP indicator, featuring possible separation from water after the analysis. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Nanosilica-based molecularly imprinted polymer nanoshell for specific recognition and determination of rhodamine B in red wine and beverages.

    Science.gov (United States)

    Long, Zerong; Xu, Weiwei; Lu, Yi; Qiu, Hongdeng

    2016-09-01

    A new and facile rhodamine B (RhB)-imprinted polymer nanoshell coating for SiO2 nanoparticles was readily prepared by a combination of silica gel modification and molecular surface imprinting. The RhB-imprinted polymers (RhB-MIPs) were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and UV-vis spectroscopy; the binding properties and selectivity of these MIPs were investigated in detail. The uniformly imprinted nanoparticles displayed a rather thin shell thickness (23nm) with highly effective recognition sites, showing homogenous distribution and monolayer adsorption. The maximum MIP adsorption capacity (Qm) was as high as 45.2mgg(-1), with an adsorption equilibrium time of about 15min at ambient temperature. Dynamic rebinding experiments showed that chemical adsorption is crucial for RhB binding to RhB-MIPs. The adsorption isotherm for RhB-MIPs binding could also be described by the Langmuir equation at different temperatures and pH values. Increasing temperature led to an enhanced Qm, a decreased dissociation constant (K'd), and a more negative free energy (ΔG), indicating that adsorption is favored at higher temperatures. Moreover, the adsorption capacity of RhB was remarkably affected by pH. At pH>7, the adsorption of RhB was driven by hydrogen bonding interactions, while at pH<7 electrostatic forces were dominant. Additionally, the MIPs also showed specific recognition of RhB from the standard mixture solution containing five structurally analogs. This method was also successfully employed to determine RhB content in red wine and beverages using three levels of spiking, with recoveries in the range of 91.6-93.1% and relative standard deviations lower than 4.1%. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Ratiometric detection of copper ions and alkaline phosphatase activity based on semiconducting polymer dots assembled with rhodamine B hydrazide.

    Science.gov (United States)

    Sun, Junyong; Mei, Han; Gao, Feng

    2017-05-15

    The rational surface functionalization of semiconducting polymer dots (Pdots) has attracted much attention to extend their applications in fabricating chemo/biosensing platform. In this study, a novel ratiometric fluorescent sensing platform using functionalized Pdots as probes for fluorescence signal transmission has been designed for sensing Cu(Ⅱ) and activity of alkaline phosphatase (ALP) with high selectivity and enhanced sensitivity. The highly fluorescent Pdots were firstly prepared with Poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1',3}-thiadiazole)] (PFBT) via nanoprecipitation method, and then assembled with non-fluorescent rhodamine B hydrazide (RB-hy), which shows special binding activity to Cu(Ⅱ), through adsorption process to obtain functionalized nanohybrids, Pdots@RB-hy. As thus, a FRET donors/acceptors pair, in which PFBT Pdots act as energy donors while RB-hy-Cu(II) complexes act as energy acceptors were constructed. On the basis of the varies in fluorescence intensities of donors/acceptors in the presence of different amounts of Cu(II), a ratiometric method for sensing Cu(II) has been proposed. The proposed ratiometric Cu(II) sensor shows a good linear detection range from 0.05 to 5μM with a detection limit of 15nM. Furthermore, using the Pdots@RB-hy-Cu(II) system as signal transducer, a ratiometric sensing for alkaline phosphatase (ALP) activity has also been established with pyrophosphate (PPi) as substrates. The constructed ratiometric sensor of ALP activity displays a linear detection range from 0.005 to 15UL -1 with a detection limit of 0.0018UL -1 . The sensor was further successfully used for ALP activity detection in human serum with satisfactory results. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Degradation of a cationic dye (Rhodamine 6G) using hydrodynamic cavitation coupled with other oxidative agents: Reaction mechanism and pathway.

    Science.gov (United States)

    Rajoriya, Sunil; Bargole, Swapnil; Saharan, Virendra Kumar

    2017-01-01

    In the present study, decolorization and mineralization of a cationic dye, Rhodamine 6G (Rh6G), has been carried out using hydrodynamic cavitation (HC). Two cavitating devices such as slit and circular venturi were used to generate cavitation in HC reactor. The process parameters such as initial dye concentration, solution pH, operating inlet pressure, and cavitation number were investigated in detail to evaluate their effects on the decolorization efficiency of Rh6G. Decolorization of Rh6G was marginally higher in the case of slit venturi as compared to circular venturi. The kinetic study showed that decolorization and mineralization of the dye fitted first-order kinetics. The loadings of H 2 O 2 and ozone have been optimized to intensify the decolorization and mineralization efficiency of Rh6G using HC. Nearly 54% decolorization of Rh6G was obtained using a combination of HC and H 2 O 2 at a dye to H 2 O 2 molar ratio of 1:30. The combination of HC with ozone resulted in 100% decolorization in almost 5-10min of processing time depending upon the initial dye concentration. To quantify the extent of mineralization, total organic carbon (TOC) analysis was also performed using various processes and almost 84% TOC removal was obtained using HC coupled with 3g/h of ozone. The degradation by-products formed during the complete degradation process were qualitatively identified by liquid chromatography-mass spectrometry (LC-MS) and a detailed degradation pathway has been proposed. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Adsorption of chromium(VI) and Rhodamine B by surface modified tannery waste: Kinetic, mechanistic and thermodynamic studies

    Energy Technology Data Exchange (ETDEWEB)

    Anandkumar, J. [Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Mandal, B., E-mail: bpmandal@iitg.ernet.in [Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam (India)

    2011-02-28

    In this study, various activation methods have been employed to examine the potential reuse of tannery residual biomass (TRB) obtained from vegetable tanning process for the removal of Cr(VI) and Rhodamine B (RB) from aqueous solution. The maximum BET surface area (10.42 m{sup 2}/g), honey comb pore distribution and uptake of both Cr(VI) and RB were achieved when only 3-fold volume of HCl was used to activate the biomass. The pH and temperature experiment showed that they have considerable impact on the adsorption capacity of the used adsorbent. The presence of other ions (Na{sup +}, Ca{sup 2+} and NH{sub 4}{sup +}) significantly reduces the metal uptake but marginal enhancement in the dye removal was observed when Na{sup +} and NH{sub 4}{sup +} ions were present in the solution. The equilibrium data fitted satisfactorily with the Langmuir model and monolayer sorption capacity obtained as 177-217 and 213-250 mg/g for Cr(VI) and RB at 30-50 deg. C, respectively. The sorption kinetics was found to follow the pseudo-second-order kinetic model. The increase in adsorption capacity for both metal and dye with increase in temperature indicates that the uptake was endothermic in nature. The results indicate that the HCl modified TRB (A-TRB) could be employed as a low cost adsorbent for the removal of both Cr(VI) and RB from the aqueous solution including industrial wastewater.

  12. Amphiphilic HPMA-LMA copolymers increase the transport of Rhodamine 123 across a BBB model without harming its barrier integrity.

    Science.gov (United States)

    Hemmelmann, Mirjam; Metz, Verena V; Koynov, Kaloian; Blank, Kerstin; Postina, Rolf; Zentel, Rudolf

    2012-10-28

    The successful non-invasive treatment of diseases associated with the central nervous system (CNS) is generally limited by poor brain permeability of various developed drugs. The blood-brain barrier (BBB) prevents the passage of therapeutics to their site of action. Polymeric drug delivery systems are promising solutions to effectively transport drugs into the brain. We recently showed that amphiphilic random copolymers based on the hydrophilic p(N-(2-hydroxypropyl)-methacrylamide), pHPMA, possessing randomly distributed hydrophobic p(laurylmethacrylate), pLMA, are able to mediate delivery of domperidone into the brain of mice in vivo. To gain further insight into structure-property relations, a library of carefully designed polymers based on p(HPMA) and p(LMA) was synthesized and tested applying an in vitro BBB model which consisted of human brain microvascular endothelial cells (HBMEC). Our model drug Rhodamine 123 (Rh123) exhibits, like domperidone, a low brain permeability since both substances are recognized by efflux transporters at the BBB. Transport studies investigating the impact of the polymer architecture in relation to the content of hydrophobic LMA revealed that random p(HPMA)-co-p(LMA) having 10mol% LMA is the most auspicious system. The copolymer significantly increased the permeability of Rh123 across the HBMEC monolayer whereas transcytosis of the polymer was very low. Further investigations on the mechanism of transport showed that integrity and barrier function of the BBB model were not harmed by the polymer. According to our results, p(HPMA)-co-p(LMA) copolymers are a promising delivery system for neurological therapeutics and their application might open alternative treatment strategies. Copyright © 2012 Elsevier B.V. All rights reserved.

  13. A simple and rapid chromatographic method to determine unauthorized basic colorants (rhodamine B, auramine O, and pararosaniline) in processed foods.

    Science.gov (United States)

    Tatebe, Chiye; Zhong, Xining; Ohtsuki, Takashi; Kubota, Hiroki; Sato, Kyoko; Akiyama, Hiroshi

    2014-09-01

    A simple and rapid high-performance liquid chromatography (HPLC) method to determine basic colorants such as pararosaniline (PA), auramine O (AO), and rhodamine B (RB) in various processed foods was developed. Linearity of the calibration curves ranged from 0.05 to 50 μg/mL for PA and 0.05-100 μg/mL for AO and RB. The detection and quantification limits (LOD and LOQ) of the basic colorants, which were evaluated as signal-to-noise ratios of 3 for LOD and 10 for LOQ, ranged from 0.0125 to 0.05 and 0.025 to 0.125 μg/g, respectively. The recoveries and relative standard deviations of three basic colorants in six processed foods, namely, chili sauce, curry paste, gochujang (hot pepper paste), tandoori chicken (roasted chicken prepared with yogurt and spices), powder soup, and shrimp powder ranged from 70.2% to 102.8% and 0.8% to 8.0%, respectively. The intraday precision of the recovery test ranged from 1.7% to 4.5%, whereas the interday precision ranged from 3.7% to 7.7%. The reported method has been successfully applied to basic colorant determination in various processed foods such as fat-based food matrices (curry paste and tandoori chicken), chili products (gochujang and chili sauce), and protein-based products (shrimp powder and powder soup). Thin layer chromatography and liquid chromatography/mass spectrometry methods for the determination of basic colorants in processed foods were also developed for rapid analysis and identification, respectively. These methods are very useful for monitoring unauthorized basic colorants in inspection centers or quarantine laboratories in many countries.

  14. Photodegradation of rhodamine B and methyl orange over one-dimensional TiO2 catalysts under simulated solar irradiation

    International Nuclear Information System (INIS)

    Guo Changsheng; Xu Jian; He Yan; Zhang Yuan; Wang Yuqiu

    2011-01-01

    In this paper, two one-dimensional (1D) TiO 2 nanostructures, nanotube and nanowire were synthesized by a hydrothermal method using Degussa P25 TiO 2 as a precursor. The synthesized anatase TiO 2 nanotubes with the diameters of 10-20 nm and length of several hundred nanometers were formed from P25 and NaOH with the hydrothermal treatment temperature at 150 deg. C, and anatase TiO 2 nanowires with the diameters of 10-40 nm and length up to several micrometers were prepared at 180 deg. C. The photocatalytic activity of the two nanostructures was evaluated by degrading rhodamine B (RhB) and methyl orange (MO) in aqueous solutions under simulated solar light irradiation. The results suggested that the TiO 2 nanocatalysts displayed higher degradation activity compared to P25. For RhB, 98.9% and 91.9% of RhB were removed by nanotubes and nanowires, respectively after 60 min irradiation in comparison to the 81.8% removal by P25. Similar trend was observed for MO, with the removal percentage of 95.6%, 88.3% and 74.9%, respectively by TiO 2 nanotubes, nanowires and P25. Meanwhile, RhB and MO showed different photodegradation rates in nanotubes and nanowires suspensions, probably due to the morphology and crystal structure of the TiO 2 nanocatalysts which play important roles in the degradation activity of the catalysts.

  15. Argon laser phototherapy of human malignancies using rhodamine-123 as a new laser dye: The intracellular role of oxygen

    International Nuclear Information System (INIS)

    Castro, D.J.; Saxton, R.E.; Markley, J.; Foote, C.S.; Fetterman, H.R.; Castro, D.J.; Ward, P.H.

    1990-01-01

    Recent studies demonstrated that the cationic, mitochondrial-specific dye Rhodamine-123 (Rh-123), is an efficient tumor photosensitizer for Argon laser treatment of human cancer cells both in vitro and in tumors grown as xenografts in athymic mice. To demonstrate the photodynamic mechanism of action of this reaction, the intracellular role of oxygen and temperature changes in treated cells have to be defined. In the current study, a large panel of human tumor cell lines of diverse histologic origin were tested for in vitro sensitivity to Rh-123 and the Argon laser (514.5 nm) in oxygen, deuterium oxide (D2O), and nitrogen (N2) environment. Tumor cells in suspension were first sensitized to Rh-123 (1 or 20 micrograms/ml for 1 hour), cooled on ice to 4 degrees C, and then exposed to the Argon laser (delta T = 14 +/- 1 degree C). Cell proliferation measured by [3H]-thymidine uptake 24 hours after sensitization with Rh-123 and laser treatment was significantly decreased in tumor cells kept in oxygen and D2O atmospheres. No decrease in DNA synthesis was seen in Rh-123 and laser treated cells kept in an N2 environment. Control tumor cells treated with Rh-123 or the Argon laser separately did not show any decreased [3H]-thymidine uptake in oxygen, D2O or N2 environment. These results provide evidence of a photodynamic process since Rh-123 sensitization and Argon laser activation occur at nonthermal levels of energy and are oxygen dependent. The high effectiveness of this technique of photodynamic therapy with the Argon laser, and low toxicity of Rh-123 could make its clinical use very attractive for the treatment of superficial malignancies

  16. Extraction spectrophotometric determination of elements in form of ion-association compounds of metal chaltes and dyes. Pt. 2. The system cobalt-5, 7-dichloro-8-hydroxyquinoline-rhodamine 6G, benzene

    International Nuclear Information System (INIS)

    Minczewski, J.; Chwastowska, J.; Lachowicz, E.

    1976-01-01

    The extraction of the ion-pair formed by the anionic, coordinatively saturated complex of cobalt with 5,7-dichloro-8-hydroxyquinoline (Cl 2 HOx) and Rhodamine 6G cation (ROD + ) was investigated. The cobalt ions at the concentration 4 x 10 -5 M are fully extractable at pH=6.3; 1 x 10 -3 M Cl 2 HOx and 4 x 10 -4 M Rhodamine 6G after 10 minutes of extraction. In absence of Rhodamine 6G the cobalt complexes are not extractable. The extractable ion-pair has the composition according to the formula Co(Cl 2 Ox) 3 - .ROD + . The molar absorptivity at lambda=542 nm is equal to 7.5 x 10 4 . The extraction constant log Ksub(ex)=0.44+-0.14. The protonation constant of Rhodamine (log K=-0.98+-0.08) and two-phase stability constant of the carbinol base of Rhodamine 6G (log β = 5.35. +- 0.04) were also calculated. (author)

  17. Comparitive study of fluorescence lifetime quenching of rhodamine 6G by MoS2 and Au-MoS2

    Science.gov (United States)

    Shakya, Jyoti; Kasana, Parath; Mohanty, T.

    2018-04-01

    Time resolved fluorescence study of Rhodamine 6G (R6G) in the presence of Molybdenum disulfide (MoS2) nanosheets and gold doped MoS2 (Au-MoS2) have been carried out and discussed. We have analyzed the fluorescence decay curves of R6G and it is observed that Au-MoS2 is a better fluorescence lifetime quencher as compare to MoS2 nanosheets. Also, the energy transfer efficiency and energy transfer rate from R6G to MoS2 and Au-MoS2 has been calculated and found higher for Au-MoS2.

  18. RBAP, a Rhodamine B-Based Derivative: Synthesis, Crystal Structure Analysis, Molecular Simulation, and Its Application as a Selective Fluorescent Chemical Sensor for Sn2+

    Directory of Open Access Journals (Sweden)

    Xiaofeng Bao

    2014-06-01

    Full Text Available A new fluorescent chemosensor based on a Rhodamine B and a benzyl 3-aminopropanoate conjugate (RBAP was designed, synthesized, and structurally characterized. Its single crystal structure was obtained and analyzed by X-ray analysis. In a MeOH/H2O (2:3, v/v, pH 5.95 solution RBAP exhibits a high selectivity and excellent sensitivity for Sn2+ ions in the presence of many other metal cations. The binding analysis using the Job’s plot suggested the RBAP formed a 1:1 complex with Sn2+.

  19. Measurement of Fluorescence in a Rhodamine-123 Doped Self-Assembled “Giant” Mesostructured Silica Sphere Using a Smartphone as Optical Hardware

    Directory of Open Access Journals (Sweden)

    Ingemar Petermann

    2011-07-01

    Full Text Available The blue OLED emission from a mobile phone was characterised, revealing a sharp emission band centred at λ = 445 nm with a 3dB bandwidth Δλ ~ 20 nm. It was used to excite Rhodamine 123 doped within a “giant” mesostructured silica sphere during fabrication through evaporative self-assembly of silica nanoparticles. Fluorescence was able to be detected using a standard optical microscope fitted with a green transmission pass filter and cooled CCD and with 1 ms exposure time demonstrating the potential of mobile platforms as the basis for portable diagnostics in the field.

  20. RBAP, a Rhodamine B-Based Derivative: Synthesis, Crystal Structure Analysis, Molecular Simulation, and Its Application as a Selective Fluorescent Chemical Sensor for Sn2+

    OpenAIRE

    Xiaofeng Bao; Xiaowei Cao; Xuemei Nie; Yanyan Jin; Baojing Zhou

    2014-01-01

    A new fluorescent chemosensor based on a Rhodamine B and a benzyl 3-aminopropanoate conjugate (RBAP) was designed, synthesized, and structurally characterized. Its single crystal structure was obtained and analyzed by X-ray analysis. In a MeOH/H2O (2:3, v/v, pH 5.95) solution RBAP exhibits a high selectivity and excellent sensitivity for Sn2+ ions in the presence of many other metal cations. The binding analysis using the Job’s plot suggested the RBAP formed a 1:1 complex with Sn2+.

  1. Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications.

    OpenAIRE

    Belov, V.; Mitronova, G.; Bossi, M.; Boyarski, V.; Hebisch, E.; Geisler, C.; Kolmakov, K.; Wurm, C.; Willig, K.; Hell, S.

    2014-01-01

    Caged rhodamine dyes (Rhodamines NN) of five basic colors were synthesized and used as “hidden” markers in subdiffractional and conventional light microscopy. These masked fluorophores with a 2-diazo-1-indanone group can be irreversibly photoactivated, either by irradiation with UV- or violet light (one-photon process), or by exposure to intense red light (λ∼750 nm; two-photon mode). All dyes possess a very small 2-diazoketone caging group incorporated into the 2-diazo-1-indanone residue with...

  2. Optimization of a heterogeneous catalytic hydrodynamic cavitation reactor performance in decolorization of Rhodamine B: application of scrap iron sheets.

    Science.gov (United States)

    Basiri Parsa, Jalal; Ebrahimzadeh Zonouzian, Seyyed Alireza

    2013-11-01

    A low pressure pilot scale hydrodynamic cavitation (HC) reactor with 30 L volume, using fixed scrap iron sheets, as the heterogeneous catalyst, with no external source of H2O2 was devised to investigate the effects of operating parameters of the HC reactor performance. In situ generation of Fenton reagents suggested an induced advanced Fenton process (IAFP) to explain the enhancing effect of the used catalyst in the HC process. The reactor optimization was done based upon the extent of decolorization (ED) of aqueous solution of Rhodamine B (RhB). To have a perfect study on the pertinent parameters of the heterogeneous catalyzed HC reactor, the following cases as, the effects of scrap iron sheets, inlet pressure (2.4-5.8 bar), the distance between orifice plates and catalyst sheets (submerged and inline located orifice plates), back-pressure (2-6 bar), orifice plates type (4 various orifice plates), pH (2-10) and initial RhB concentration (2-14 mg L(-1)) have been investigated. The results showed that the highest cavitational yield can be obtained at pH 3 and initial dye concentration of 10 mg L(-1). Also, an increase in the inlet pressure would lead to an increase in the ED. In addition, it was found that using the deeper holes (thicker orifice plates) would lead to lower ED, and holes with larger diameter would lead to the higher ED in the same cross-sectional area, but in the same holes' diameters, higher cross-sectional area leads to the lower ED. The submerged operation mode showed a greater cavitational effects rather than the inline mode. Also, for the inline mode, the optimum value of 3 bar was obtained for the back-pressure condition in the system. Moreover, according to the analysis of changes in the UV-Vis spectra of RhB, both degradation of RhB chromophore structure and N-deethylation were occurred during the catalyzed HC process. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. The Effects of Heteroatoms Si and S on Tuning the Optical Properties of Rhodamine- and Fluorescein-Based Fluorescence Probes: A Theoretical Analysis.

    Science.gov (United States)

    Zhou, Panwang; Ning, Cai; Alsaedi, Ahmed; Han, Keli

    2016-10-05

    The effects of the incorporated heteroatoms Si and S on tuning the optical properties of rhodamine- and fluorescein-based fluorescence probes is investigated using DFT and time-dependent DFT with four different functionals. As previously proposed, the large redshift (90 nm) produced by a Si atom in both the absorption and emission spectra can be attributed to the σ*-π* conjugation between the σ* orbital of the Si atom and the π* orbital of the adjacent carbon atoms. However, the presence of a Si atom does not alter the fluorescence quenching mechanism of the nonfluorescent forms of the investigated compounds. For the first time, these theoretical results indicate that the n orbital of the S atom plays an important role in determining the optical properties of the nonfluorescent form of rhodamine-based fluorescence probes. It alters the fluorescence quenching mechanism by lowering the energy of the dark nπ* state, which is due to breakage of the C10-S52 bond upon photoexcitation. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. A highly selective and sensitive photoswitchable fluorescent probe for Hg2+ based on bisthienylethene-rhodamine 6G dyad and for live cells imaging.

    Science.gov (United States)

    Xu, Li; Wang, Sheng; Lv, Yingnian; Son, Young-A; Cao, Derong

    2014-07-15

    A new photochromic diarylethene derivative bearing rhodamine 6G dimmer as a fluorescent molecular probe is designed and synthesized successfully. All the compounds are characterized by nuclear magnetic resonance and mass spectrometry. The bisthienylethene-rhodamine 6G dyad exhibit excellent phtochromism with reversibly color and fluorescence changes alternating irradiation with ultraviolet and visible light. Upon addition of Hg(2+), its color changes from colorless to red and its fluorescence is remarkably enhanced. Whereas other ions including K(+), Na(+), Ca(2+), Mg(2+), Fe(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Mn(2+), Pb(2+), Ni(2+), Fe(3+), Al(3+), Cr(3+) and so on induce basically no spectral changes, which constitute a highly selective and sensitive photoswitchable fluorescent probe toward Hg(2+). Furthermore, by means of laser confocal scanning microscopy experiments, it is demonstrated that this probe can be applied for live cell imaging and monitoring Hg(2+) in living lung cancer cells with satisfying results, which shows its value of potential application in environmental and biological systems. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.

    Science.gov (United States)

    Alam, Rabiul; Islam, Abu Saleh Musha; Sasmal, Mihir; Katarkar, Atul; Ali, Mahammad

    2018-05-10

    A new sensor (L3) based on Rhodamine-B-en (2) and 2-(pyridin-2-ylmethoxy)benzaldehyde (1) has been developed for highly sensitive and selective recognition of NO in purely aqueous medium where the reaction of NO with the fluorophore leads to an unusual formation of nitrosohydroxylamine with the selective opening of the spirolactam ring over different cations, anions, amino-acids and other biological species with prominent enhancement in absorption and emission intensities. A large enhancement of fluorescence intensity for NO (11 fold) was observed upon addition of 3 equivalents of NO into the sensor in aqueous HEPES buffer (20 mM) at pH 7.20, μ = 0.05 M NaCl with naked eye detection. The corresponding Kf value was evaluated to be (7.55 ± 2.04) × 104 M-1 from the fluorescence titration plot. Quantum yields of L3 and the [L3 + NO] compound are found to be 0.07 and 0.77, respectively, using Rhodamine-6G as the standard. The LOD for NO was determined by the 3σ method and found to be 83.4 nM. The L3 sensor has low cytotoxicity, and is cell permeable and suitable for in vitro NO sensing. The in vivo compatibility of the sensor was also checked on zebrafish.

  6. Application of Micro-cloud point extraction for spectrophotometric determination of Malachite green, Crystal violet and Rhodamine B in aqueous samples

    Science.gov (United States)

    Ghasemi, Elham; Kaykhaii, Massoud

    2016-07-01

    A novel, green, simple and fast method was developed for spectrophotometric determination of Malachite green, Crystal violet, and Rhodamine B in water samples based on Micro-cloud Point extraction (MCPE) at room temperature. This is the first report on the application of MCPE on dyes. In this method, to reach the cloud point at room temperature, the MCPE procedure was carried out in brine using Triton X-114 as a non-ionic surfactant. The factors influencing the extraction efficiency were investigated and optimized. Under the optimized condition, calibration curves were found to be linear in the concentration range of 0.06-0.60 mg/L, 0.10-0.80 mg/L, and 0.03-0.30 mg/L with the enrichment factors of 29.26, 85.47 and 28.36, respectively for Malachite green, Crystal violet, and Rhodamine B. Limit of detections were between 2.2 and 5.1 μg/L.

  7. Photophysical property of rhodamine-cored poly(amidoamine) dendrimers: Simultaneous effect of spirolactam ring-opening and PET process on sensing trivalent chromium ion

    International Nuclear Information System (INIS)

    Lei Yonglin; Su Yuanqiang; Huo Jichuan

    2011-01-01

    Two novel poly(amidoamine) (PAMAM) dendrimers, comprising rhodamine B unit in the core and 1-phenyl-3-methyl-5-pyrazolone unit at the periphery, have been synthesized and characterized. Both dendrimers displayed high selectivity and sensitivity towards Cr 3+ ion. As considering the potential of being applied as fluorescent sensors for Cr 3+ ion, we studied the complexes formed between the dendrimers and Cr 3+ ion. Different PAMAM dendrimers had different recognition mechanism towards Cr 3+ ion. For dendrimer G2, the recognition of Cr 3+ was mainly due to the ring-opening of spirolactam. However, it significantly depended on the simultaneous effect of ring-opening of spirolactam and photoinduced electron transfer (PET) in the case of dendrimer G3. - Highlights: → First synthesize two novel PAMAM simultaneously containing rhodamine and pyrazolone. → Novel dendrimer show high selectivity and sensitivity towards Cr 3+ . → Recognition Cr 3+ of dendrimer G2 is dominantly due to the ring-opening mechanism. → Sensing Cr 3+ of dendrimer G3 is dependent on simultaneous mechanisms of ring-opening and PET.

  8. Computational design and fabrication of core-shell magnetic molecularly imprinted polymer for dispersive micro-solid-phase extraction coupled with high-performance liquid chromatography for the determination of rhodamine 6G.

    Science.gov (United States)

    Xie, Jin; Xie, Jie; Deng, Jian; Fang, Xiangfang; Zhao, Haiqing; Qian, Duo; Wang, Hongjuan

    2016-06-01

    A novel core-shell magnetic nano-adsorbent with surface molecularly imprinted polymer coating was fabricated and then applied to dispersive micro-solid-phase extraction followed by determination of rhodamine 6G using high-performance liquid chromatography. The molecularly imprinted polymer coating was prepared by copolymerization of dopamine and m-aminophenylboronic acid (functional monomers), in the presence of rhodamine 6G (template). The selection of the suitable functional monomers was based on the interaction between different monomers and the template using the density functional theory. The ratios of the monomers to template were further optimized by an OA9 (3(4) ) orthogonal array design. The binding performances of the adsorbent were evaluated by static, kinetic, and selective adsorption experiments. The results reveal that the adsorbent possesses remarkable affinity and binding specificity for rhodamine 6G because of the enhanced Lewis acid-base interaction between the B(Ш) embedded in the imprinted cavities and the template. The nano-adsorbent was successfully applied to dispersive micro-solid-phase extraction coupled to high-performance liquid chromatography for the trace determination of rhodamine 6G in samples with a detection limit of 2.7 nmol/L. Spiked recoveries ranged from 93.0-99.1, 89.5-92.7, and 86.9-105% in river water, matrimony vine and paprika samples, respectively, with relative standard deviations of less than 4.3%. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Ag(I)-bovine serum albumin hydrosol-mediated formation of Ag3PO4/reduced graphene oxide composites for visible-light degradation of Rhodamine B solution.

    Science.gov (United States)

    Ma, Peiyan; Chen, Anliang; Wu, Yan; Fu, Zhengyi; Kong, Wei; Che, Liyuan; Ma, Ruifang

    2014-03-01

    A cost-effective Ag(I)-bovine serum albumin (BSA) supramolecular hydrosol strategy was utilized to assemble Ag3PO4 nanospheres onto reduced graphene oxide (rGO) sheets. The obtained composites were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, UV-vis absorption spectroscopy and Fourier transform infrared spectroscopy. Compared with the pure Ag3PO4 crystals and Ag3PO4 particles prepared with Ag(I)-BSA hydrosol as precursor, the Ag3PO4/rGO composites obtained with different content of graphene oxide indicated improved visible-light-driven photocatalysis activity for the decomposition of Rhodamine B aqueous solution. The results pointed to the possibility of synthesizing graphene-based photocatalysts by metal ion-BSA hydrosol. Copyright © 2013 Elsevier Inc. All rights reserved.

  10. Synthesis of a ternary Ag/RGO/ZnO nanocomposite via microwave irradiation and its application for the degradation of Rhodamine B under visible light.

    Science.gov (United States)

    Surendran, Divya Kollikkara; Xavier, Marilyn Mary; Viswanathan, Vandana Parakkal; Mathew, Suresh

    2017-06-01

    Reduced graphene oxide supporting plasmonic photocatalyst (Ag) on ZnO has been synthesized via a facile two-step microwave synthesis using RGO/ZnO and AgNO 3 . First step involves fabrication of RGO/ZnO via microwave irradiation. The nanocomposites were characterized by X-ray diffraction analysis, transmission electron microscopy, Fourier transform infrared spectroscopy, and Raman spectroscopy. Ag/RGO/ZnO shows enhanced photoactivity under visible light for the degradation of Rhodamine B. Enhanced charge separation and migration have been assigned using UV-vis diffuse reflectance spectra, photoluminescence spectra, electrochemical impedance spectra, and TCSPC analysis. The improved photoactivity of Ag/RGO/ZnO can be ascribed to the prolonged lifetime of photogenerated electron-hole pairs and effective interfacial hybridization between RGO and Ag with ZnO nanoparticles. Ag nanoparticles can absorb visible light via surface plasmon resonance to enhance photocatalytic activity.

  11. Detection of gain enhancement in laser-induced fluorescence of rhodamine B lasing dye by silicon dioxide nanostructures-coated cavity

    Science.gov (United States)

    Al-Tameemi, Mohammed N. A.

    2018-03-01

    In this work, nanostructured silicon dioxide films are deposited by closed-field unbalanced direct-current (DC) reactive magnetron sputtering technique on two sides of quartz cells containing rhodamine B dye dissolved in ethanol with 10‒5 M concentration as a random gain medium. The preparation conditions are optimized to prepare highly pure SiO2 nanostructures with a minimum particle size of about 20 nm. The effect of SiO2 films as external cavity for the random gain medium is determined by the laser-induced fluorescence of this medium, and an increase of about 200% in intensity is observed after the deposition of nanostructured SiO2 thin films on two sides of the dye cell.

  12. Highly Selective and Ultrasensitive Turn-On Luminescence Chemosensor for Mercury (II) Determination Based on the Rhodamine 6G Derivative FC1 and Au Nanoparticles

    Science.gov (United States)

    Brasca, Romina; Onaindia, María C.; Goicoechea, Héctor C.; Muñoz de la Peña, Arsenio; Culzoni, María J.

    2016-01-01

    A method for the detection and quantitation of Hg2+ in aqueous samples by fluorescence spectroscopy is presented. It consists of a turn-on sensor developed by coupling Gold nanoparticles (AuNPs) with the rhodamine 6G derivative FC1, in which the response is generated by a mercury-induced ring-opening reaction. The AuNPs were included in order to improve the sensitivity of the method towards the analyte, maintaining its high selectivity. The method was validated in terms of linearity, precision and accuracy, and applied to the quantitation of Hg2+ in Milli-Q and tap water with and without spiked analyte. The limit of detection and quantitation were 0.15 μg·L−1 and 0.43 μg·L−1, respectively, constituting a substantial improvement of sensitivity in comparison with the previously reported detection of Hg2+ with free FC1. PMID:27782059

  13. Two emissive-magnetic composite platforms for Hg(II) sensing and removal: The combination of magnetic core, silica molecular sieve and rhodamine chemosensors

    Science.gov (United States)

    Mao, Hanping; Liu, Zhongshou

    2018-01-01

    In this paper, a composite sensing platform for Hg(II) optical sensing and removal was designed and reported. A core-shell structure was adopted, using magnetic Fe3O4 nanoparticles as the core, silica molecular sieve MCM-41 as the shell, respectively. Two rhodamine derivatives were synthesized as chemosensor and covalently immobilized into MCM-41 tunnels. Corresponding composite samples were characterized with SEM/TEM images, XRD analysis, IR spectra, thermogravimetry and N2 adsorption/desorption analysis, which confirmed their core-shell structure. Their emission was increased by Hg(II), showing emission turn on effect. High selectivity, linear working curves and recyclability were obtained from these composite samples.

  14. Förster resonance energy transfer between α -Bi2O3 nanorods and rhodamine 6G in aqueous media for turn-off glucose-sensing application

    International Nuclear Information System (INIS)

    Balasubramanian, Karthikeyan

    2017-01-01

    The present study deals with the Förster resonance energy transfer (FRET) process that takes place between α -Bi 2 O 3 nanorods (BN), which function as a donor, and Rhodamine 6G, which (R6G) functions as an acceptor. Fluorescence of R6G situated at 550 nm is found to be enhanced due to this FRET behaviour with the increase of the number of acceptor concentrations. Apart from this, the rate of energy transfer also increased. It is found that a decrease in the donor–acceptor distance is assisting FRET. Steady state and time-resolved fluorescence studies are performed in two different excitation wavelengths and it shows the possible FRET behaviour where the average lifetime increases with the increase of acceptor concentrations. This pair is used to sense the glucose in nM concentrations. (paper)

  15. Enhanced photosensitization process induced by the p–n junction of Bi2O2CO3/BiOCl heterojunctions on the degradation of rhodamine B

    International Nuclear Information System (INIS)

    Lu, Haijing; Xu, Lingling; Wei, Bo; Zhang, Mingyi; Gao, Hong; Sun, Wenjun

    2014-01-01

    Herein, we report the enhanced photosensitization process in the nanosheet Bi 2 O 2 CO 3 /BiOCl heterojunctions photocatalyst. The combined XRD, FT-IR and Raman results have confirmed the co-existence of Bi 2 O 2 CO 3 and BiOCl phases in the composites. Although both Bi 2 O 2 CO 3 and BiOCl are wide bandgap semiconductors, the composites showed an unexpectedly high catalytic activity in decomposing RhB (rhodamine B) aqueous solution under visible light irradiation. The mechanism of enhanced photocatalytic activity was ascribed to the inner electric field formed in the Bi 2 O 2 CO 3 /BiOCl p–n junction.

  16. Synthesis and properties of complexes of copper(II), nickel(II), cobalt(II) and uranyl ions with 3-(p-tolylsulphonamido)rhodamine

    International Nuclear Information System (INIS)

    El-Bindary, A.A.; El-Sonbati, A.Z.

    2000-01-01

    Metal complexes of copper(II), nickel(II), cobalt(II) and uranyl ions with 3-(p-tolylsulphonamido)rhodamine (HL) have been prepared and characterized by chemical and thermal analyses, molar conductivity , magnetic susceptibility measurements, and infrared, electronic and EPR spectra. The visible and EPR spectra indicated that the Cu(II) complex has a tetragonal geometry. From EPR spectrum of the Cu(II) complex,various parameters were calculated. The crystal field parameters of Ni(II) complex were calculated and were found to agree fairly well with the values reported for known square pyramidal complexes. The infrared spectral studies showed a monobasic bidentate behaviour with the oxygen and nitrogen donor system. Thermal stabilities of the complexes are also reported. (author)

  17. Rhodamine B immobilized on hollow Au-HMS material for naked-eye detection of Hg{sup 2+} in aqueous media

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Na [State Key Laboratory of Fine Chemicals, College of Chemical Engineering, Dalian University of Technology, Dalian, 116024 (China); Li, Gang, E-mail: liganghg@dlut.edu.cn [State Key Laboratory of Fine Chemicals, College of Chemical Engineering, Dalian University of Technology, Dalian, 116024 (China); Cheng, Zhuhong [State Key Laboratory of Fine Chemicals, College of Chemical Engineering, Dalian University of Technology, Dalian, 116024 (China); Zuo, Xiujin [Key Laboratory of Bio-organic Chemistry, College of Environmental and Chemical Engineering, Dalian University, Dalian, 116622 (China)

    2012-08-30

    Graphical abstract: Au-HMS-Probe with worm-like mesoporous framework for detection of Hg{sup 2+} in aqueous media has been simply and effectively synthesized by immobilizing a Rhodamine B derivative on Au-HMS via Au-N groups under room temperature. Highlights: Black-Right-Pointing-Pointer Au-HMS-Probe is prepared via Au-N bonds. Black-Right-Pointing-Pointer Gold nanoparticles are chosen as connectors instead of silane agents. Black-Right-Pointing-Pointer Au-HMS is chosen as carrier for the first time. Black-Right-Pointing-Pointer The immobilization method of Au-HMS-Probe is very simple and effective. Black-Right-Pointing-Pointer Au-HMS-Probe shows excellent ability for detecting Hg{sup 2+} by naked-eye. - Abstract: A simple, effective method has been demonstrated to immobilize Rhodamine B (RhB) probes on mesoporous silica (Au-HMS). The prepared chemosensor (Au-HMS-Probe) was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), UV-vis spectrum and Fourier transform infrared spectroscopy (FT-IR). Further application of Au-HMS-Probe in sensing Hg{sup 2+} was confirmed by fluorescence titration experiment. Au-HMS-Probe afforded 'turn-on' fluorescence enhancement and displayed high brightness in water, and it also showed excellent selectivity for Hg{sup 2+} over alkali (Na{sup +}, K{sup +}), alkaline earth (Mg{sup 2+}, Ca{sup 2+}) and other heavy metal ions (Ag{sup +}, Cd{sup 2+}, Co{sup 2+}, Pb{sup 2+}, Ni{sup 2+}, Cu{sup 2+}, Fe{sup 2+}). Importantly, Au-HMS-Probe could be regenerated by treatment with tetrapropylammonium hydroxide solution.

  18. Ag3PO4/ZnO: An efficient visible-light-sensitized composite with its application in photocatalytic degradation of Rhodamine B

    International Nuclear Information System (INIS)

    Liu, Wei; Wang, Mingliang; Xu, Chunxiang; Chen, Shifu; Fu, Xianliang

    2013-01-01

    Graphical abstract: The free OH radicals generated in the VB of ZnO play the primary role in the visible-light photocatalytic degradation of RhB in Ag 3 PO 4 /ZnO system. The accumulated electrons in the CB of Ag 3 PO 4 can be transferred to O 2 adsorbed on the surface of the composite semiconductors and H 2 O 2 yields. H 2 O 2 reacts with electrons in succession to produce active ·OH to some extent. Display Omitted Highlights: ► Efficient visible-light-sensitized Ag 3 PO 4 /ZnO composites were successfully prepared. ► Effect of Ag 3 PO 4 content on the catalytic activity of Ag 3 PO 4 /ZnO is studied in detail. ► Rate constant of RhB degradation over Ag 3 PO 4 (3.0 wt.%)/ZnO is 3 times that of Ag 3 PO 4 . ► The active species in RhB degradation are examined by adding a series of scavengers. ► Visible light degradation mechanism of RhB over Ag 3 PO 4 /ZnO is systematically studied. -- Abstract: The efficient visible-light-sensitized Ag 3 PO 4 /ZnO composites with various weight percents of Ag 3 PO 4 were prepared by a facile ball milling method. The photocatalysts were characterized by XRD, DRS, SEM, EDS, XPS, and BET specific area. The ·OH radicals produced during the photocatalytic reaction was detected by the TA–PL technique. The photocatalytic property of Ag 3 PO 4 /ZnO was evaluated by photocatalytic degradation of Rhodamine B under visible light irradiation. Significantly, the results revealed that the photocatalytic activity of the composites was much higher than that of pure Ag 3 PO 4 and ZnO. The rate constant of RhB degradation over Ag 3 PO 4 (3.0 wt.%)/ZnO is 3 times that of single-phase Ag 3 PO 4 . The optimal percentage of Ag 3 PO 4 in the composite is 3.0 wt.%. It is proposed that the ·OH radicals produced in the valence band of ZnO play the leading role in the photocatalytic degradation of Rhodamine B by Ag 3 PO 4 /ZnO systems under visible light irradiation.

  19. Simultaneous determination of 2,4,6-trichlorophenol and pentachlorophenol based on poly(Rhodamine B)/graphene oxide/multiwalled carbon nanotubes composite film modified electrode

    International Nuclear Information System (INIS)

    Zhu, Xiaolin; Zhang, Kexin; Lu, Nan; Yuan, Xing

    2016-01-01

    Graphical abstract: A poly(Rhodamine B)/graphene oxide/multiwalled carbon nanotubes composite film modified glassy carbon electrode (PRhB/GO/MWCNTs/GCE) was developed for the simultaneous determination of 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP) without any pretreatment. - Highlights: • A poly(RhB)/graphene oxide/multiwalled carbon nanotubes composite was synthesized. • The composite film was characterized by SEM, XRD, EIS and Raman spectroscopy. • The simultaneous electrochemical determination of 2,4,6-TCP and PCP was realized. • The electrode showed high sensitivity, excellent reproducibility and good stability. • The electrode was used to determine 2,4,6-TCP and PCP in practical water samples. - Abstract: In the present study, a poly(Rhodamine B)/graphene oxide/multiwalled carbon nanotubes nanocomposite modified glass carbon electrode (PRhB/GO/MWCNTs/GCE) was developed for the simultaneous determination of 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP). The PRhB/GO/MWCNTs film was extensively characterized by emission scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of 2,4,6-TCP and PCP were investigated by cyclic voltammetry, linear sweep voltammetry and differential pulse voltammetry. Due to the synergistic effect, the PRhB/GO/MWCNTs/GCE significantly facilitated the simultaneous electro-oxidation of 2,4,6-TCP and PCP with peak potential difference of 160 mV and enhanced oxidation currents. Under optimum conditions, the oxidation current of 2,4,6-TCP was linear to its concentration in the ranges of 4.0 × 10"−"9 to 1.0 × 10"−"7 M and 1.0 × 10"−"7 to 1.0 × 10"−"4 M with the detection limit (S/N = 3) of 8.0 × 10"−"1"0 M. And the linear concentration ranges for PCP were 2.0 × 10"−"9 to 1.0 × 10"−"7 M and 1.0 × 10"−"7 to 9.0 × 10"−"5 M with the detection limit of 5.0 × 10"−"1"0 M

  20. Synthesis, surface group modification of 3D MnV{sub 2}O{sub 6} nanostructures and adsorption effect on Rhodamine B

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wanqun, E-mail: wqz@ustc.edu.cn [Hefei National Laboratory for Physical Sciences at Micro-scale, University of Science and Technology of China, Hefei, Anhui 230026 (China); Chemical Experimental Teaching Center, University of Science and Technology of China, Hefei, Anhui 230026 (China); Shi, Lei, E-mail: shil@ustc.edu.cn [Hefei National Laboratory for Physical Sciences at Micro-scale, University of Science and Technology of China, Hefei, Anhui 230026 (China); Tang, Kaibin; Liu, Zhongping [Hefei National Laboratory for Physical Sciences at Micro-scale, University of Science and Technology of China, Hefei, Anhui 230026 (China)

    2012-07-15

    Highlights: ► Fabrication of urchin-like MnV{sub 2}O{sub 6} with oxygen-containing surface groups. ► Mn{sub 0.5}V{sub 2}O{sub 5}·nH{sub 2}O as an intermediate product holds the key to the final products. ► 3D architectures of MnV{sub 2}O{sub 6} with oxygen-containing surface groups as sorbent. ► The sorbent shows a good adsorption ability. -- Abstract: Highly uniform 3D MnV{sub 2}O{sub 6} nanostructures modified by oxygen functional groups (-COO-) were successfully prepared in large quantities by an approach involving preparation of vanadyl ethylene glycolate as the precursor. The growth and self-assembly of MnV{sub 2}O{sub 6} nanobelts and nanorods could be readily tuned by additive species and quantities, which brought different morphologies and sizes to the final products. With a focus on the regulation of structure, the formation process of 3D architectures of MnV{sub 2}O{sub 6} by self-assembly of nanobelts was followed by field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD). The consecutive processes of vanadyl ethylene glycolate and benzoyl peroxide assisted formation of layered structure Mn{sub 0.5}V{sub 2}O{sub 5}·nH{sub 2}O, growth of aligned MnV{sub 2}O{sub 6} nanobelts, and oriented assembly were proposed for the growth mechanism. The band gap vs. different morphology was also studied. Optical characterization of these MnV{sub 2}O{sub 6} with different morphologies showed direct bandgap energies at 1.8–1.95 eV. The adsorption properties of 3D MnV{sub 2}O{sub 6} nanostructures synthesized under different conditions were investigated through the removal test of Rhodamine B in aqueous water, and the 3D nanostructures synthesized with 30 g L{sup −1} benzoyl peroxide showed good adsorption capability of Rhodamine B.

  1. Simultaneous determination of 2,4,6-trichlorophenol and pentachlorophenol based on poly(Rhodamine B)/graphene oxide/multiwalled carbon nanotubes composite film modified electrode

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Xiaolin; Zhang, Kexin; Lu, Nan; Yuan, Xing, E-mail: yuanx@nenu.edu.cn

    2016-01-15

    Graphical abstract: A poly(Rhodamine B)/graphene oxide/multiwalled carbon nanotubes composite film modified glassy carbon electrode (PRhB/GO/MWCNTs/GCE) was developed for the simultaneous determination of 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP) without any pretreatment. - Highlights: • A poly(RhB)/graphene oxide/multiwalled carbon nanotubes composite was synthesized. • The composite film was characterized by SEM, XRD, EIS and Raman spectroscopy. • The simultaneous electrochemical determination of 2,4,6-TCP and PCP was realized. • The electrode showed high sensitivity, excellent reproducibility and good stability. • The electrode was used to determine 2,4,6-TCP and PCP in practical water samples. - Abstract: In the present study, a poly(Rhodamine B)/graphene oxide/multiwalled carbon nanotubes nanocomposite modified glass carbon electrode (PRhB/GO/MWCNTs/GCE) was developed for the simultaneous determination of 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP). The PRhB/GO/MWCNTs film was extensively characterized by emission scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of 2,4,6-TCP and PCP were investigated by cyclic voltammetry, linear sweep voltammetry and differential pulse voltammetry. Due to the synergistic effect, the PRhB/GO/MWCNTs/GCE significantly facilitated the simultaneous electro-oxidation of 2,4,6-TCP and PCP with peak potential difference of 160 mV and enhanced oxidation currents. Under optimum conditions, the oxidation current of 2,4,6-TCP was linear to its concentration in the ranges of 4.0 × 10{sup −9} to 1.0 × 10{sup −7} M and 1.0 × 10{sup −7} to 1.0 × 10{sup −4} M with the detection limit (S/N = 3) of 8.0 × 10{sup −10} M. And the linear concentration ranges for PCP were 2.0 × 10{sup −9} to 1.0 × 10{sup −7} M and 1.0 × 10{sup −7} to 9.0 × 10{sup −5} M with the

  2. In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B

    Directory of Open Access Journals (Sweden)

    Antoine Berberi

    2014-01-01

    Full Text Available Objectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization of the interfacial gap may ultimately lead to infection and bone resorption. Using Rhodamine B, a sensitive fluorescent tracer dye, we aim in this study to investigate leakage at implant-abutment connection of three implant systems having the same prosthetic interface. Materials and Methods. Twenty-one implants (seven Astra Tech, seven Euroteknika, and seven Dentium with the same prosthetic interface were connected to their original abutments, according to the manufacturers’ recommendation. After determination of the inner volume of each implant systems, the kinetic quantification of leakage was evaluated for each group using Rhodamine B (10−2 M. For each group, spectrophotometric analysis was performed to detect leakage with a fluorescence spectrophotometer at 1 h (T0 and 48 h (T1 of incubation time at room temperature. Results. Astra Tech had the highest inner volume (6.8 μL, compared to Dentium (4 μL and Euroteknika (2.9 μL. At T0 and T1, respectively, the leakage volume and percentage of each system were as follows: Astra Tech 0.043 μL or 1.48% (SD 0.0022, 0.08 μL or 5.56% (SD 0.0074, Euroteknika 0.09 μL or 6.93% (SD 0.0913, 0.21 μL or 20.55% (SD 0.0035, and Dentium 0.07 μL or 4.6% (SD 0.0029, 0.12 μL or 10.47% (SD 0.0072. Conclusion. The tested internal conical implant-abutment connections appear to be unable to prevent leakage. In average, Astra Tech implants showed the highest inner volume and the least leakage.

  3. Magnetic solid phase extraction using ionic liquid-coated core-shell magnetic nanoparticles followed by high-performance liquid chromatography for determination of Rhodamine B in food samples.

    Science.gov (United States)

    Chen, Jieping; Zhu, Xiashi

    2016-06-01

    Three hydrophobic ionic liquids (ILs) (1-butyl-3-methylimidazole hexafluorophosphate ([BMIM]PF6), 1-hexyl-3-methyl-imidazole hexafluoro-phosphate ([HMIM]PF6), and 1-octyl-3-methylimidazole hexafluorophosphate ([OMIM]PF6)) were used to coat Fe3O4@SiO2 nanoparticles (NPs) with core-shell structures to prepare magnetic solid phase extraction (MSPE) agents (Fe3O4@SiO2@IL). A novel method of MSPE coupled with high-performance liquid chromatography for the separation/analysis of Rhodamine B was then established. The results showed that Rhodamine B was adsorbed rapidly on Fe3O4@SiO2@[OMIM]PF6 and was released using ethanol. Under optimal conditions, the pre-concentration factor for the proposed method was 25. The linear range, limit of detection (LOD), correlation coefficient (R), and relative standard deviation (RSD) were found to be 0.50-150.00 μgL(-1), 0.08 μgL(-1), 0.9999, and 0.51% (n=3, c=10.00 μgL(-1)), respectively. The Fe3O4@SiO2 NPs could be re-used up to 10 times. The method was successfully applied to the determination of Rhodamine B in food samples. Copyright © 2016. Published by Elsevier Ltd.

  4. Hydrothermal synthesis of B-doped Bi2MoO6 and its high photocatalytic performance for the degradation of Rhodamine B

    Science.gov (United States)

    Wang, Min; Han, Jin; Guo, Pengyao; Sun, Mingzhi; Zhang, Yu; Tong, Zhu; You, Meiyan; Lv, Chunmei

    2018-02-01

    B-doped Bi2MoO6 photocatalysts have been synthesized by a hydrothermal method using HBO3 as the doping source and the effect of B doping content on Bi2MoO6 structure and performance was studied. The samples were characterized with XPS, XRD, SEM, BET, UV-Vis DRS, and PL. The photocatalytic activity was evaluated by photocatalytic degradation of Rhodamine B (RhB) under visible light (λ ≥ 420 nm). The results show that all samples are orthorhombic structure. Doping Bi2MoO6 with B increases the amount of Bi5+ and oxygen vacancies, which led to stronger absorption in visible light region and lower band gap energy of the B-doped Bi2MoO6 but had little impact on morphology. B doping significantly improves the photocatalytic activity of Bi2MoO6 and the highest photocatalytic degradation rate is 89% when the initial molar ratio of B to Bi is 0.01.

  5. Removal of Rhodamine B under visible irradiation in the presence of Fe⁰, H₂O₂, citrate and aeration at circumneutral pH.

    Science.gov (United States)

    Hong, Jun; Lu, Sijia; Zhang, Caixiang; Qi, Shihua; Wang, Yanxin

    2011-09-01

    A new Vis-Fe(0)-H(2)O(2)-citrate-O(2) system comprising zero-valent iron, hydrogen peroxide, citrate anion and aeration at circumneutral pH under visible irradiation was studied. 21 μmol L(-1) of Rhodamine B (RhB) was chosen as the substrate to be tested. Experiments were conducted under conditions of 2.9 mmol L(-1) of H(2)O(2), 12.6g of Fe(0) and 1.0 mmol L(-1) of citrate at pH 7.5. Results showed that, in 1h reaction, 54% of RhB was removed with corresponding 26% of COD reduced. Meanwhile, the amount of released dissolved irons from Fe(0) surface was found to be at a very low level as removal in 3h reaction. Control and factor influencing experiments showed that the prohibitive extents of individual factor importance on RhB removal followed a decreasing order of Fe(0)>H(2)O(2)>citrate>Vis>O(2). This study showed an excellent system that could remove refractory organic compounds from water in laboratory researches, and also provided a good idea to reduce secondary contamination by dissolved irons in future investigations. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Preparation of TiO₂/Carbon Nanotubes/Reduced Graphene Oxide Composites with Enhanced Photocatalytic Activity for the Degradation of Rhodamine B.

    Science.gov (United States)

    Huang, Yanzhen; Chen, Dongping; Hu, Xinling; Qian, Yingjiang; Li, Dongxu

    2018-06-13

    In this report, ternary titanium dioxide (TiO₂)/carbon nanotubes (CNTs)/reduced graphene oxide (rGO) composites were fabricated by a facile and environmentally friendly one-pot solvethermal method for the removal of Rhodamine B (RhB). Its structures were represented by X-ray powder diffraction (XRD), Raman spectrometry, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The photocatalytic performance was tested by the degradation efficiency of RhB under UV-vis light irradiation. The experimental results indicated that photocatalytic activity improved as the ratio of CNTs:TiO₂ ranged from 0.5% to 3% but reduced when the content increased to 5% and 10%, and the TiO₂/CNTs/rGO-3% composites showed superior photocatalytic activity compared with the binary ones (i.e., TiO₂/CNTs, TiO₂/rGO) and pristine TiO₂. The rate constant k of the pseudo first-order reaction was about 1.5 times that of TiO₂. The improved photocatalytic activity can be attributed to the addition of rGO and CNTs, which reduced the recombination of photo-induced electron-hole pairs, and the fact that CNTs and rGO, with a high specific surface area and high adsorption ability to efficiently adsorb O₂, H₂O and organics, can increase the hydroxyl content of the photocatalyst surface.

  7. Sunlight impelled photocatalytic pursuance of Ag-TiO2-SGO and Pt-TiO2-SGO ternary nanocomposites on rhodamine B degradation

    Science.gov (United States)

    Alamelu, K.; Ali, B. M. Jaffar

    2018-04-01

    We demonstrate a hydrothermal method combined with polyol reduction process for the synthesis of an Ag-TiO2-SGO and Pt-TiO2-SGO ternary nanocomposites in which the Ag, Pt and TiO2 nanoparticles are dispersed on the Sulfonated graphene oxide nanosheets. The structural and optical properties of obtained nanocomposites were characterized by XRD, UV-DRS, Raman, FTIR and Photoluminescence spectroscopy. The nanocomposites shows increased light absorption ability in the visible region due to surface plasmon resonance effect of noble metal. The rate of electron-hole pair recombination was significating reduced for nanocomposites system compare to pure. Also, their Performance for the photocatalytic degradation of Rhodamine B as a model organic pollutant is explored. The results showed that Ag-TiO2-SGO and Pt-TiO2-SGO nanocomposites could degrade 95% of the dye within 90 min, under natural sunlight irradiation. The reaction kinetics of ternary nanocomposites exhibit more than 2.2 fold increased photocatalytic activity compared to pristine TiO2. Sulfonated graphene based ternary photocatalyst are potential candidates for wastewater treatment in real time application, due to this ability degrade cationic and anionic dyes.

  8. Activatable Optical Imaging with a Silica-Rhodamine Based Near Infrared (SiR700) Fluorophore: A comparison with cyanine based dyes

    Science.gov (United States)

    McCann, Thomas E.; Kosaka, Nobuyuki; Koide, Yuichiro; Mitsunaga, Makoto; Choyke, Peter L.; Nagano, Tetsuo; Urano, Yasuteru; Kobayashi, Hisataka

    2011-01-01

    Optical imaging is emerging as an important tool to visualize tumors. However, there are many potential choices among the available fluorophores. Optical imaging probes that emit in the visible range can image superficial tumors with high quantum yields, however, if deeper imaging is needed then near infrared (NIR) fluorophores are necessary. Most commercially available NIR fluorophores are cyanine based and are prone to non-specific binding and relatively limited photostability. Silica-containing rhodamine (SiR) fluorophores represent a new class of NIR fluorophores, which permit photoactivation via H-dimer formation as well as demonstrate improved photostability. This permits higher tumor-to-background ratios (TBRs) to be achieved over longer periods of time. Here, we compared an avidin conjugated with SiR700 (Av-SiR700) to similar compounds based on cyanine dyes (Av-Cy5.5 and Av-Alexa Fluor 680) in a mouse tumor model of ovarian cancer metastasis. We found that the Av-SiR700 probe demonstrated superior quenching enabling activation after binding-internalization to the target cell. As a result, Av-SiR700 had higher TBRs compared to Av-Cy5.5, and better biostability compared to Av-Alexa Fluor 680. PMID:22034863

  9. Synthesis of molecularly imprinted polypyrrole/titanium dioxide nanocomposites and its selective photocatalytic degradation of rhodamine B under visible light irradiation

    Directory of Open Access Journals (Sweden)

    M. Q. He

    2014-11-01

    Full Text Available Highly selective molecularly imprinted nanocomposites MIPRhB-PPy/TiO2 were successfully prepared by surface molecular imprinting technique with rhodamine B (RhB as template molecule. The prepared MIPRhB-PPy/TiO2 coated with a thin imprinted layer could respond to visible light. The static and dynamic binding experiments revealed that MIPRhB-PPy/TiO2 possessed strong affinity, high adsorption capacity and fast adsorption rate for RhB. The selectivity experiments indicated that MIPRhB-PPy/TiO2 had excellent recognition selectivity for RhB. Selective photocatalytic degradation experiments indicated that the apparent rate constant (k for the photodegradation of RhB over MIPRhB-PPy/TiO2 is 0.0158 min–1, being 3.6 times of that over non-imprinted nanocomposites NIPRhB-PPy/TiO2 (0.0044 min–1. Compared with the NIPRhB-PPy/TiO2, MIPRhB-PPy/TiO2 showed higher photocatalytic selectivity toward RhB under visible light, which was attributed the introduction of the imprinted cavities on the surface of MIPRhB-PPy/TiO2. Moreover, MIPRhB-PPy/TiO2 exhibited high reusability and stability. The results indicate that molecularly imprinted nanocomposites MIPRhB-PPy/TiO2 have a promising perspective in industrial wastewater treatment.

  10. Nanocomposite of exfoliated bentonite/g-C3N4/Ag3PO4 for enhanced visible-light photocatalytic decomposition of Rhodamine B.

    Science.gov (United States)

    Ma, Jianfeng; Huang, Daiqin; Zhang, Wenyi; Zou, Jing; Kong, Yong; Zhu, Jianxi; Komarneni, Sridhar

    2016-11-01

    Novel visible-light-driven heterojunction photocatalyst comprising exfoliated bentonite, g-C3N4 and Ag3PO4 (EB/g-C3N4/Ag3PO4) was synthesized by a facile and green method. The composites EB/g-C3N4/Ag3PO4 were characterized by X-ray diffraction, Transmission electron microscopy, Fourier transform infrared spectroscopy, UV-Vis diffuse reflectance spectroscopy and the Brunauer, Emmett, and Teller (BET) surface area method. Under visible light irradiation, EB/g-C3N4/Ag3PO4 composites displayed much higher photocatalytic activity than that of either pure g-C3N4 or pure Ag3PO4 in the degradation of Rhodamine B (RhB). Among the hybrid photocatalysts, EB/g-C3N4/Ag3PO4 composite containing 20 wt% Ag3PO4 exhibited the highest photocatalytic activity for the decolorization of RhB. Under the visible-light irradiation, the RhB dye was completely decolorized in less than 60 min. The enhanced photocatalytic performance is attributed to the stable structure, enlarged surface area, strong adsorbability, strong light absorption ability, and high-efficiency separation rate of photoinduced electron-hole pairs. Our finding paves a way to design highly efficient and stable visible-light-induced photocatalysts for practical applications in wastewater treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Chip-based molecularly imprinted monolithic capillary array columns coated GO/SiO2 for selective extraction and sensitive determination of rhodamine B in chili powder.

    Science.gov (United States)

    Zhai, Haiyun; Huang, Lu; Chen, Zuanguang; Su, Zihao; Yuan, Kaisong; Liang, Guohuan; Pan, Yufang

    2017-01-01

    A novel solid-phase extraction chip embedded with array columns of molecularly imprinted polymer-coated silanized graphene oxide (GO/SiO2-MISPE) was established to detect trace rhodamine B (RB) in chili powder. GO/SiO2-MISPE monolithic columns for RB detection were prepared by optimizing the supporting substrate, template, and polymerizing monomer under mild water bath conditions. Adsorption capacity and specificity, which are critical properties for the application of the GO/SiO2-MISPE monolithic column, were investigated. GO/SiO2-MIP was examined by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy. The recovery and the intraday and interday relative standard deviations for RB ranged from 83.7% to 88.4% and 2.5% to 4.0% and the enrichment factors were higher than 110-fold. The chip-based array columns effectively eliminated impurities in chili powder, indicating that the chip-based GO/SiO2-MISPE method was reliable for RB detection in food samples using high-performance liquid chromatography. Accordingly, this method has direct applications for monitoring potentially harmful dyes in processed food. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Evaluation in zebrafish model of the toxicity of rhodamine B-conjugated crotamine, a peptide potentially useful for diagnostics and therapeutics.

    Science.gov (United States)

    Chan, Judy Yuet-Wa; Zhou, Hefeng; Kwan, Yiu Wa; Chan, Shun Wan; Radis-Baptista, Gandhi; Lee, Simon Ming-Yuen

    2017-11-01

    Crotamine is defensin-like cationic peptide from rattlesnake venom that possesses anticancer, antimicrobial, and antifungal properties. Despite these promising biological activities, toxicity is a major concern associated with the development of venom-derived peptides as therapeutic agents. In the present study, we used zebrafish as a system model to evaluate the toxicity of rhodamine B-conjugated (RhoB) crotamine derivative. The lethal toxic concentration of RhoB-crotamine was as low as 4 μM, which effectively kill zebrafish larvae in less than 10 min. With non-lethal concentrations (<1 μM), crotamine caused malformation in zebrafish embryos, delayed or completely halted hatching, adversely affected embryonic developmental programming, decreased the cardiac functions, and attenuated the swimming distance of zebrafish. The RhoB-crotamine translocated across vitelline membrane and accumulated in zebrafish yolk sac. These results demonstrate the sensitive responsivity of zebrafish to trial crotamine analogues for the development of novel therapeutic peptides with improved safety, bioavailability, and efficacy profiles. © 2017 Wiley Periodicals, Inc.

  13. Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg²⁺ with different sensing properties and its application in living cells.

    Science.gov (United States)

    Rui, Qing-Qing; Zhou, Yi; Fang, Yuan; Yao, Cheng

    2016-04-15

    Two new rhodamine B-based fluorescent probes PyRbS and PyRbO containing pyrene moiety were designed and synthesized. Both of the probes showed colorimetric and fluorometric sensing abilities for Hg(2+) with high selectivity over other metal ions. The binding analysis using Job's plot suggested 1:1 stoichiometry for the complexes formed for Hg(2+). Compared with PyRbO, the PyRbS showed higher selectivity and sensitivity due to the thiophilic property of Hg(2+) ion. The PyRbS exhibited the linear fluorescence quenching to Hg(2+) in the range of 0.3 to 4.8 μM (λ(ex)=365 nm) and 0.3 to 5.4 μM (λ(ex)=515 nm), and the detection limit was 0.72 μM. Moreover, ratiometric changes of PyRbS with Hg(2+) in absorption spectrum were observed, which could not be obtained in the combination of PyRbO with Hg(2+). In addition, the methyl thiazolyl tetrazolium (MTT) assay demonstrated that RbPyS had low cytotoxicity and was successfully used to monitor intracellular Hg(2+) levels in living cells. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. A new "off-on" fluorescent probe for Al(3+) in aqueous solution based on rhodamine B and its application to bioimaging.

    Science.gov (United States)

    Huang, Qi; Zhang, Qingyou; Wang, Enze; Zhou, Yanmei; Qiao, Han; Pang, Lanfang; Yu, Fang

    2016-01-05

    In this paper, a new fluorescent probe has been synthesized and applied as "off-on" sensor for the detection of Al(3+) with a high sensitivity and excellent selectivity in aqueous media. The sensor was easily prepared by one step reaction between rhodamine B hydrazide and pyridoxal hydrochloride named RBP. The structure of the sensor has been characterized by nuclear magnetic resonance and electron spray ionization-mass spectrometry. The fluorescence intensity and absorbance for the sensor showed a good linearity with the concentration of Al(3+) in the range of 0-12.5μM and 8-44μM, respectively, with detection limits of 0.23μM and 1.90μM. The sensor RBP was preliminarily applied to the determination of Al(3+) in water samples from the lake of Henan University and tap water with satisfying results. Moreover, it can be used as a bioimaging reagent for imaging of Al(3+) in living cells. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Green synthesis of zero-valent Fe-nanoparticles: Catalytic degradation of rhodamine B, interactions with bovine serum albumin and their enhanced antimicrobial activities.

    Science.gov (United States)

    Khan, Zaheer; Al-Thabaiti, Shaeel Ahmad

    2018-03-01

    Biomimetic method was used for the synthesis of Fe-nanoparticles (FeNPs). FeCl 3 and Hibiscus sabdariffa, Roselle flower aqueous extract (HBS) were employed in the present studies. The FeNPs have been characterized by using UV-visible spectroscopy, transmission electron microscope (TEM), and energy dispersion X-ray spectroscopy (EDS). The average particles diameter was found to be 18 nm. The as prepared FeNPs were used as a catalyst to the oxidative degradation of rhodamine B (RB) in presence of NaBH 4 . The effects of various quencher on the degradation rates were examined by employing ammonium oxalate (AO), benzoquinone (BQ), isopropyl alcohol (IPA), and potassium iodide (KI). The interactions of FeNPs with bovine serum albumin (BSA) have been determined and discussed. Adsorption of FeNPs into the core of BSA changes the tryptophan environment from hydrophobic to hydrophilic (from folding to partially folded and/or unfolded). Tryptophan residues, indole moieties of BSA were responsible to complex formation with FeNPs in excited states via electrostatic, van der Waals, hydrogen bonding, hydrophobic and hydrophilic interactions with static quenching. The antimicrobial activities of FeNPs have been determined against human pathogens. Hibiscus sabdariffa flower extract shows mild antimicrobial activities against all target pathogenic organisms. FeNPs have potential antimicrobial activity against both bacterial strains and candida fungus even at low concentration, and retains potential application in biomedical industries. Copyright © 2018 Elsevier B.V. All rights reserved.

  16. Photocatalytic degradation of rhodamine B, paracetamol and diclofenac sodium by supported titania-based catalysts from petrochemical residue: effect of doping with magnesium.

    Science.gov (United States)

    da Silva, William Leonardo; Lansarin, Marla Azário; Dos Santos, João Henrique Z; Silveira, Fernando

    2016-11-01

    Three different lots of a residual Ziegler-Natta catalyst slurry (bearing Ti and Mg) obtained from an industrial petrochemical plant were employed as sources for the photocatalyst supported on silica. The effect of additional magnesium (1.0-25.0 wt% Mg/SiO 2 ) on the photocatalytic properties of the doped materials was investigated. Doping the titania-based photocatalyst with Mg results in a shift in the absorption threshold toward the visible spectrum. The optical band gap energy of the bare supported photocatalyst was in the range of 2.5 eV and shifted to 1.72 eV after 25 wt% Mg doping. The systems were evaluated for the photodegradation of one dye (rhodamine B (RhB)) and two drugs (paracetamol and diclofenac sodium) either under ultraviolet (UV) (365 nm - UVA) or visible radiation, separately. Among the evaluated systems, doping with 25 wt% Mg afforded the highest degradation values for the target molecules under UV and visible radiation (i.e. 87%, 60% and 55% of the RhB, paracetamol and diclofenac under UV, respectively, and 82%, 48.3% and 48% under visible irradiation, respectively).

  17. Dynamics of Intercalation/De-Intercalation of Rhodamine B during the Polymorphic Transformation of CdAl Layered Double Hydroxide to the Brucite-Like Cadmium Hydroxide

    KAUST Repository

    Saliba, Daniel

    2016-06-23

    Cadmium-Aluminum layered double hydroxide (CdAl LDH) is thermodynamically unstable and transforms to Cd(OH)2 and Al(OH)3 in a short period of time. We present a reaction-diffusion framework that enables us to use in situ steady-state fluorescence spectroscopy to study the kinetics of intercalation of a fluorescent probe (Rhodamine B (RhB)) during the formation of the CdAl LDH and its de-intercalation upon the conversion of the LDH phase to the β phase (Cd(OH)2). The method involves the diffusion of sodium hydroxide into a hydrogel gel matrix containing the aluminum and cadmium ions as well as the species we wish to incorporate in the interlayers of the LDH. The existence of RhB between the LDH layers and its expel during the transition into the β phase are proved via fluorescence microscopy, XRD and ssNMR. The activation energies of intercalation and de-intercalation of RhB are computed and show dependence on the cationic ratio of the corresponding LDH. We find that the energies of de- intercalation are systematically higher than those of intercalation proving that the dyes are stabilized due to the probe-brucite sheets interactions.

  18. High photocatalytic activity of hierarchical SiO2@C-doped TiO2 hollow spheres in UV and visible light towards degradation of rhodamine B.

    Science.gov (United States)

    Zhang, Ying; Chen, Juanrong; Hua, Li; Li, Songjun; Zhang, Xuanxuan; Sheng, Weichen; Cao, Shunsheng

    2017-10-15

    Ongoing research activities are targeted to explore high photocatalytic activity of TiO 2 -based photocatalysts for the degradation of environmental contaminants under UV and visible light irradiation. In this work, we devise a facile, cost-effective technique to in situ synthesize hierarchical SiO 2 @C-doped TiO 2 (SCT) hollow spheres for the first time. This strategy mainly contains the preparation of monodisperse cationic polystyrene spheres (CPS), sequential deposition of inner SiO 2 , the preparation of the sandwich-like CPS@SiO 2 @CPS particles, and formation of outer TiO 2 . After the one-step removal of CPS templates by calcination at 450°C, hierarchical SiO 2 @C-doped TiO 2 hollow spheres are in situ prepared. The morphology, hierarchical structure, and properties of SCT photocatalyst were characterized by TEM. SEM, STEM Mapping, BET, XRD, UV-vis spectroscopy, and XPS. Results strongly confirm the carbon doping in the outer TiO 2 lattice of SCT hollow spheres. When the as-synthesized SCT hollow spheres were employed as a photocatalyst for the degradation of Rhodamine B under visible-light and ultraviolet irradiation, the SCT photocatalyst exhibits a higher photocatalytic activity than commercial P25, effectively overcoming the limitations of poorer UV activity for many previous reported TiO 2 -based photocatalysts due to doping. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Inorganic-organic Ag-rhodamine 6G hybrid nanorods: "turn on" fluorescent sensors for highly selective detection of Pb2+ ions in aqueous solution.

    Science.gov (United States)

    Tyagi, A K; Ramkumar, Jayshree; Jayakumar, O D

    2012-02-07

    Lead metal ions are of great concern and the monitoring of their concentration in the environment has become extremely important. In the present study, a new inorganic-organic hybrid assay of Ag nanorods (AgNR)-Rhodamine 6G (R6G) was developed for the sensitive and selective determination of Pb(2+) ions in aqueous solutions. To the best of our knowledge there is almost no literature on the use of silver nanorod sensors for determination of lead ions in aqueous solutions. The sensor is developed by the coating of R6G on the surface of AgNRs. The sensing is based on the photoluminescence of R6G. The sensor was rapid as the measurements were carried out within 3 min of addition of the test solution to the AgNR-R6G hybrid. Moreover, the system showed excellent stability at tested concentration levels of Pb(2+) ions. The naked eye detection of the colour was possible with 1 mg L(-1) of Pb(2+) ions. The present method has a detection limit of 50 μg L(-1) of Pb(2+) (for a signal/noise (S/N) ratio > 3). The selectivity toward Pb(2+) ions against other metal ions was improved using chelating agents. The proposed method was validated by analysis using different techniques.

  20. Solvothermal synthesis of mesoporous slabstone-like TiO{sub 2} and its photodegradation preference in a methyl orange-rhodamine B mixture solution

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Kun; Zhu, Lianjie; Li, Naixuan; Sun, Youguang; Li, Hongbin; Zhu, Xuchen [School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384 (China)

    2012-10-15

    Mesoporous slabstone-like anatase TiO{sub 2} micro-nanometer composite structure has been successfully synthesized by a facile solvothermal method at 180 C using polyethylene glycol (PEG) as a structure-directing agent, followed by calcination at 400 C for 2 h. The crystal structure and morphology of the product were characterized by XRD, SEM, TEM and HRTEM. Its BET specific surface area was obtained from N{sub 2} adsorption-desorption isotherm measurement. Rhodamine B (RB) aqueous solution was used to evaluate the photocatalytic activity of the as-prepared TiO{sub 2} under simulated sunlight irradiation and compared with that of commercial TiO{sub 2} (P25). A RB and methyl orange (MO) coexisting solution was chosen to investigate the photodegradation preference of the slabstone-like TiO{sub 2} on these two dyes. The results show that the photocatalytic activity of the as-prepared TiO{sub 2} is much higher than that of P25, and MO is the preferential degradation species in the MO-RB mixture solution. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  1. Regenerable, innovative porous silicon-based polymer-derived ceramics for removal of methylene blue and rhodamine B from textile and environmental waters.

    Science.gov (United States)

    Bruzzoniti, Maria Concetta; Appendini, Marta; Onida, Barbara; Castiglioni, Michele; Del Bubba, Massimo; Vanzetti, Lia; Jana, Prasanta; Sorarù, Gian Domenico; Rivoira, Luca

    2018-04-01

    The presence of residual color in treated textile wastewater above the regulation limits is still a critical issue in many textile districts. Innovative, polymer-derived ceramics of the Si-C-O system were here synthesized in order to obtain porous nanocomposite materials where a free carbon phase is dispersed into a silicon carbide/silicon oxycarbide network. The sorbents were comprehensively characterized for the removal of two model water-soluble dyes (i.e., the cation methylene blue and the zwitterion rhodamine B). Adsorption is very rapid and controlled by intra-particle and/or film diffusion, depending on dye concentration. Among the nanocomposites studied, the SiOC aerogel (total capacity about 45 mg/g, is easily regenerated under mild treatment (250 °C, 2 h). Adsorption of dyes is not affected by the matrix composition: removals of 150 mg/L methylene blue from river water and simulated textile wastewater with high content of metal ions (2-50 mg/L) and chemical oxygen demand (800 mg/L) were higher than 92% and quantitative for a dye concentration of 1 mg/L.

  2. A sensitive and selective fluorescence assay for metallothioneins by exploiting the surface energy transfer between rhodamine 6G and gold nanoparticles

    International Nuclear Information System (INIS)

    Yan, Yu-Qian; Tang, Xian; Wang, Yong-Sheng; Li, Ming-Hui; Cao, Jin-Xiu; Chen, Si-Han; Zhu, Yu-Feng; Wang, Xiao-Feng; Huang, Yan-Qin

    2015-01-01

    We report on a sensitive and selective strategy for the determination of metallothioneins (MTs). The assay is based on the suppression of the surface energy transfer that occurs between rhodamine 6G (Rh6G) and gold nanoparticles (AuNPs). If Rh6G is adsorbed onto the surface of AuNPs in water solution of pH 3.0, its fluorescence is quenched due to surface energy transfer. However, on addition of MTs to the Rh6G-AuNPs system, fluorescence is recovered owing to the formation of the MTs-AuNPs complex and the release of Rh6G into the solution. Under optimized conditions, the increase in fluorescence intensity is directly proportional to the concentration of the MTs in the range from 9.68 to 500 ng mL −1 , with a detection limit as low as 2.9 ng mL −1 . The possible mechanism of this assay is discussed. The method was successfully applied to the determination of MTs in (spiked) human urine. (author)

  3. Polypyrrole-magnetite dispersive micro-solid-phase extraction combined with ultraviolet-visible spectrophotometry for the determination of rhodamine 6G and crystal violet in textile wastewater.

    Science.gov (United States)

    Kamaruddin, Amirah Farhan; Sanagi, Mohd Marsin; Wan Ibrahim, Wan Aini; Md Shukri, Dyia S; Abdul Keyon, Aemi S

    2017-11-01

    Polypyrrole-magnetite dispersive micro-solid-phase extraction method combined with ultraviolet-visible spectrophotometry was developed for the determination of selected cationic dyes in textile wastewater. Polypyrrole-magnetite was used as adsorbent due to its thermal stability, magnetic properties, and ability to adsorb Rhodamine 6G and crystal violet. Dispersive micro-solid-phase extraction parameters were optimized, including sample pH, adsorbent amount, extraction time, and desorption solvent. The optimum polypyrrole-magnetite dispersive micro-solid phase-extraction conditions were sample pH 8, 60 mg polypyrrole-magnetite adsorbent, 5 min of extraction time, and acetonitrile as the desorption solvent. Under the optimized conditions, the polypyrrole-magnetite dispersive micro-solid-phase extraction with ultraviolet-visible method showed good linearity in the range of 0.05-7 mg/L (R 2  > 0.9980). The method also showed a good limit of detection for the dyes (0.05 mg/L) and good analyte recoveries (97.4-111.3%) with relative standard deviations extraction and determination of dyes at trace concentration levels. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Random laser based on Rhodamine 6G (Rh6G doped poly(methyl methacrylate (PMMA films coating on ZnO nanorods synthesized by hydrothermal oxidation

    Directory of Open Access Journals (Sweden)

    Hua Zhang

    Full Text Available Random laser based on Rh6G doped PMMA thin films coating on ZnO nanorods synthesized by a simple hydrothermal oxidation method has been demonstrated. This kind of random laser medium is based on waveguide structure consisting of ZnO nanorods, Rh6G doped PMMA film and air. By controlling the time of hydrothermal oxidation reaction, wheat-like and hexagonal prism ZnO nanorods have been successfully fabricated. The emission spectra of these gain mediums based on different ZnO nanorods are different. The one based on wheat-like ZnO nanorods mainly exhibits amplified spontaneous emission, and the other one based on hexagonal prism ZnO nanorods shows random laser emission. The threshold of the random laser medium is about 73.8 μJ/pulse, and the full width at half maximum (FWHM is around 2.1 nm. The emission spectra measured at different detecting angles reveal that the output direction is strongly confined in ±30° by the waveguide effect. Our experiments demonstrate a promising method to achieve organic random laser medium. Keywords: Random laser, ZnO nanorods, Hydrothermal oxidation, Rhodamine 6G (Rh6G, Poly(methyl methacrylate (PMMA

  5. Chemiluminescence from an oxidation reaction of rhodamine B with cerium(IV) in a reversed micellar medium of cetyltrimethylammonium chloride in 1-hexanol-cyclohexane/water.

    Science.gov (United States)

    Hasanin, Tamer H A; Tsunemine, Yusuke; Tsukahara, Satoshi; Okamoto, Yasuaki; Fujiwara, Terufumi

    2011-01-01

    The chemiluminescence (CL) emission, observed when rhodamine B (RB) in 1-hexanol-cyclohexane was mixed with cerium(IV) sulfate in sulfuric acid dispersed in a reversed micellar medium of cetyltrimethylammonium chloride (CTAC) in 1-hexanol-cyclohexane/water, was investigated using a flow-injection system. The CL emission from the oxidation reaction of RB with Ce(IV) was found to be stronger in the CTAC reversed micellar solution compared with an aqueous solution. Bearing on the enhancement effect of the CTAC reverse micelles on the RB-Ce(IV) CL, several studies including stopped-flow, fluorescence and electron spin resonance (ESR) spectrometries were performed. Rapid spectral changes of an intermediate in the RB-Ce(IV) reaction in the aqueous and reversed micellar solutions were successfully observed using a stopped-flow method. The effect of the experimental variables, i.e., oxidant concentration, sulfuric acid concentration, the mole fraction of 1-hexanol, water-to-surfactant molar concentration ratio, flow rate, upon the CL intensity was evaluated. Under the experimental conditions optimized for a flow-injection determination of RB based on the new reversed micellar-mediated CL reaction with Ce(IV), a detection limit of 0.08 µmol dm(-3) RB was achieved, and a linear calibration graph was obtained with a dynamic range from 0.5 to 20 µmol dm(-3). The relative standard deviation (n = 6) obtained at an RB concentration of 3 µmol dm(-3) was 3%.

  6. Photocatalytic activity of attapulgite-TiO2-Ag3PO4 ternary nanocomposite for degradation of Rhodamine B under simulated solar irradiation

    Science.gov (United States)

    He, Hongcai; Jiang, Zhuolin; He, Zhaoling; Liu, Tao; Li, Enzhu; Li, Bao-Wen

    2018-01-01

    An excellent ternary composite photocatalyst consisting of silver orthophosphate (Ag3PO4), attapulgite (ATP), and TiO2 was synthesized, in which heterojunction was formed between dissimilar semiconductors to promote the separation of photo-generated charges. The ATP/TiO2/Ag3PO4 composite was characterized by SEM, XRD, and UV-vis diffuse reflectance spectroscopy. The co-deposition of Ag3PO4 and TiO2 nanoparticles onto the surface of ATP forms a lath-particle structure. Compared with composite photocatalysts consisting of two phases, ATP/TiO2/Ag3PO4 ternary composite exhibits greatly improved photocatalytic activity for degradation of rhodamine B under simulated solar irradiation. Such ternary composite not only improves the stability of Ag3PO4, but also lowers the cost by reducing application amount of Ag3PO4, which provides guidance for the design of Ag3PO4- and Ag-based composites for photocatalytic applications.

  7. Application of optimized large surface area date stone (Phoenix dactylifera ) activated carbon for rhodamin B removal from aqueous solution: Box-Behnken design approach.

    Science.gov (United States)

    Danish, Mohammed; Khanday, Waheed Ahmad; Hashim, Rokiah; Sulaiman, Nurul Syuhada Binti; Akhtar, Mohammad Nishat; Nizami, Maniruddin

    2017-05-01

    Box-Behnken model of response surface methodology was used to study the effect of adsorption process parameters for Rhodamine B (RhB) removal from aqueous solution through optimized large surface area date stone activated carbon. The set experiments with three input parameters such as time (10-600min), adsorbent dosage (0.5-10g/L) and temperature (25-50°C) were considered for statistical significance. The adequate relation was found between the input variables and response (removal percentage of RhB) and Fisher values (F- values) along with P-values suggesting the significance of various term coefficients. At an optimum adsorbent dose of 0.53g/L, time 593min and temperature 46.20°C, the adsorption capacity of 210mg/g was attained with maximum desirability. The negative values of Gibb ' s free energy (ΔG) predicted spontaneity and feasibility of adsorption; whereas, positive Enthalpy change (ΔH) confirmed endothermic adsorption of RhB onto optimized large surface area date stone activated carbons (OLSADS-AC). The adsorption data were found to be the best fit on the Langmuir model supporting monolayer type of adsorption of RhB with maximum monolayer layer adsorption capacity of 196.08mg/g. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Flow-injection chemiluminescence assay for ultra-trace determination of DNA using rhodamine B-Ce(IV)-DNA ternary system in sulfuric acid media

    International Nuclear Information System (INIS)

    Ma Yongjun; Zhou Min; Jin Xiaoyong; Zhang Ziyu; Teng Xiulan; Chen Hui

    2004-01-01

    A novel flow-injection chemiluminescence method for the determination of DNA at ultra-trace level has been established. In 0.8 M sulfuric acid media, the chemiluminescence of the rhodamine B-cerium (IV) or Ce(IV) system is enhanced by DNA, activated previously by imidazole-HCl buffer solution (pH 7.0). The enhanced intensity of chemiluminescence is in proportion to log DNA concentration 1.0x10 -8 to 0.1 μg ml -1 for herring sperm DNA and 2.0x10 -6 to 0.2 μg ml -1 for calf thymus DNA with 3σ detection limits of 8.3x10 -9 μg ml -1 for herring sperm DNA and 3.5x10 -7 μg ml -1 for calf thymus DNA, respectively. The relative standard deviation for 1.0x10 -4 μg ml -1 herring sperm DNA was 0.99% and 2.0x10 -3 μg ml -1 for calf thymus DNA was 1.1% (n=11). Using the optimized system, DNA contents in six synthetic samples has been determined with recoveries of 99.5-109.0%. The possible mechanism has also been studied in this paper

  9. Flow-injection chemiluminescence assay for ultra-trace determination of DNA using rhodamine B-Ce(IV)-DNA ternary system in sulfuric acid media

    Energy Technology Data Exchange (ETDEWEB)

    Ma Yongjun; Zhou Min; Jin Xiaoyong; Zhang Ziyu; Teng Xiulan; Chen Hui

    2004-01-09

    A novel flow-injection chemiluminescence method for the determination of DNA at ultra-trace level has been established. In 0.8 M sulfuric acid media, the chemiluminescence of the rhodamine B-cerium (IV) or Ce(IV) system is enhanced by DNA, activated previously by imidazole-HCl buffer solution (pH 7.0). The enhanced intensity of chemiluminescence is in proportion to log DNA concentration 1.0x10{sup -8} to 0.1 {mu}g ml{sup -1} for herring sperm DNA and 2.0x10{sup -6} to 0.2 {mu}g ml{sup -1} for calf thymus DNA with 3{sigma} detection limits of 8.3x10{sup -9} {mu}g ml{sup -1} for herring sperm DNA and 3.5x10{sup -7} {mu}g ml{sup -1} for calf thymus DNA, respectively. The relative standard deviation for 1.0x10{sup -4} {mu}g ml{sup -1} herring sperm DNA was 0.99% and 2.0x10{sup -3} {mu}g ml{sup -1} for calf thymus DNA was 1.1% (n=11). Using the optimized system, DNA contents in six synthetic samples has been determined with recoveries of 99.5-109.0%. The possible mechanism has also been studied in this paper.

  10. Dynamics of Intercalation/De-Intercalation of Rhodamine B during the Polymorphic Transformation of CdAl Layered Double Hydroxide to the Brucite-Like Cadmium Hydroxide

    KAUST Repository

    Saliba, Daniel; Ezzeddine, Alaa; Emwas, Abdul-Hamid M.; Khashab, Niveen M.; Al-Ghoul, Mazen

    2016-01-01

    Cadmium-Aluminum layered double hydroxide (CdAl LDH) is thermodynamically unstable and transforms to Cd(OH)2 and Al(OH)3 in a short period of time. We present a reaction-diffusion framework that enables us to use in situ steady-state fluorescence spectroscopy to study the kinetics of intercalation of a fluorescent probe (Rhodamine B (RhB)) during the formation of the CdAl LDH and its de-intercalation upon the conversion of the LDH phase to the β phase (Cd(OH)2). The method involves the diffusion of sodium hydroxide into a hydrogel gel matrix containing the aluminum and cadmium ions as well as the species we wish to incorporate in the interlayers of the LDH. The existence of RhB between the LDH layers and its expel during the transition into the β phase are proved via fluorescence microscopy, XRD and ssNMR. The activation energies of intercalation and de-intercalation of RhB are computed and show dependence on the cationic ratio of the corresponding LDH. We find that the energies of de- intercalation are systematically higher than those of intercalation proving that the dyes are stabilized due to the probe-brucite sheets interactions.

  11. SYNTHESIS OF MAGNETIC NANOPARTICLES OF TiO2-NiFe2O4: CHARACTERIZATION AND PHOTOCATALYTIC ACTIVITY ON DEGRADATION OF RHODAMINE B

    Directory of Open Access Journals (Sweden)

    Rahmayeni Rahmayeni

    2012-12-01

    Full Text Available Magnetic nanoparticles of TiO2-(xNiFe2O4 with x = 0.01, 0.1, and 0.3have been synthesized by mixture of titanium isopropoxide (TIP and nitric metal as precursors. The particles were characterized by XRD, SEM-EDX, and VSM. XRD pattern show the peaks at 2q = 25.3°, 38.4° and 47.9° which are referred as anatase phase of TiO2. Meanwhile NiFe2O4 phase was observed clearly for x = 0.3. The present of NiFe2O4 can prevent the transformation of TiO2 from anatase to rutile when the calcination temperature increased. Microstructure analyses by SEM show the homogeneous form and size of particles. The magnetic properties analysis by VSM indicates that TiO2-NiFe2O4 is paramagnetic behavior. TiO2 doped NiFe2O4 has higher photocatalytic activity than TiO2 synthesized for degradation of Rhodamine B in aqueous solution under solar light irradiation.

  12. Enhanced photocatalytic performance of RGO/Ag nanocomposites produced via a facile microwave irradiation for the degradation of Rhodamine B in aqueous solution

    Science.gov (United States)

    Divya, K. S.; Chandran, Akash; Reethu, V. N.; Mathew, Suresh

    2018-06-01

    A series of RGO/Ag nanocomposites with different weight addition ratios of graphene oxide (GO) have been successfully prepared in situ through the simultaneous reduction of GO and AgNO3 via a facile microwave irradiation. X-ray diffraction analysis, Fourier Transform Infrared Spectroscopy, UV-vis diffuse reflectance spectra, Scanning electron microscopy, Photoluminescence spectra, Raman spectra, Atomic Force Microscopy, X-ray photoelectron spectroscopy (XPS) and Transmission electron microscopy are employed to determine the properties of the samples. It is found that RGO/Ag nanocomposites with a proper weight addition ratios of GO exhibit higher photocatalytic activity toward liquid phase photodegradation of Rhodamine B under visible light irradiation. The improved photoactivity of RGO/Ag nanocomposites can be ascribed to the integrative synergestic effect of enhanced adsorption capacity, the prolonged lifetime of photogenerated electron-hole pairs and effective interfacial hybridization between RGO and Ag nanoparticles. This study also shows that graphene sheets act as electronic conductive channels to efficiently separate charge carriers from Ag nanoparticles.

  13. Electrostatic self-assembly of Fe3O4/GO nanocomposites and their application as an efficient Fenton-like catalyst for degradation of rhodamine B

    Science.gov (United States)

    Wang, Wenxia; He, Qi; Xiao, Kaijun; Zhu, Liang

    2018-03-01

    In the study, a two-major step involving a hydrothermal method and an electrostatic self-assembly method was adopted to synthesis Fe3O4/GO nanocomposites. The Fe3O4 nanoparticles were successfully modified with the 3-aminopropyltrimethoxy-silane and homogeneously deposited onto the surface of GO. They were used as Fenton-like catalyst to degrade Rhodamine B and displayed a higher activity compared with the pristine Fe3O4 nanoparticles, H2O2, Fe3O4/GO nanocomposite and Fe3O4/H2O2 system, demonstrating the synergistic effect between the superior adsorption properties of GO and the excellent catalytic activity of Fe3O4/H2O2 system. Besides, the possible catalytic mechanism and degradation pathway for RhB molecules by Fe3O4/GO nanocomposites and H2O2 was proposed based on the liquid chromatography-mass spectrometry (LC-MS) analysis. The result reveals that the •OH radicals should be the main actives species during catalytic degradation of RhB by the Fe3O4/GO/H2O2 system. In addition, the catalyst is reusable and shows efficiency up to 5 cycles. We believe the strategy in our work can provide insight into designing the novel catalysts for large-scale degradation of organic pollutants in the wastewater.

  14. Facile hydrothermal synthesis of Fe3O4@cellulose aerogel nanocomposite and its application in Fenton-like degradation of Rhodamine B.

    Science.gov (United States)

    Jiao, Yue; Wan, Caichao; Bao, Wenhui; Gao, He; Liang, Daxin; Li, Jian

    2018-06-01

    A magnetic cellulose aerogel-supported Fe 3 O 4 nanoparticles composite was designed as a highly efficient and eco-friendly catalyst for Fenton-like degradation of Rhodamine B (RhB). The composite (coded as Fe 3 O 4 @CA) was formed by embedding well-dispersed Fe 3 O 4 nanoparticles into the 3D structure of cellulose aerogels by virtue of a facile and cheap hydrothermal method. Comparative studies indicate that the RhB decolorization ratio is much higher in co-presence of Fe 3 O 4 and H 2 O 2 than that in presence of Fe 3 O 4 or H 2 O 2 only, revealing that the Fe 3 O 4 @CA-catalyzed Fenton-like reaction governed the RhB decolorization process. It was also found that almost 100% RhB removal was achieved in the Fenton-like system. Moreover, the composite exhibited higher catalytic activity than that of the individual Fe 3 O 4 particles. In addition, the Fe 3 O 4 @CA catalyst retained ∼97% of its ability to degrade RhB after the six successive degradation experiments, suggesting its excellent reusability. All these merits indicate that the green and low-cost catalyst with strong magnetic responsiveness possesses good potential for H 2 O 2 -driven Fenton-like treatment of organic dyestuff wastewater. Copyright © 2018 Elsevier Ltd. All rights reserved.

  15. Ferrocene-functionalized graphitic carbon nitride as an enhanced heterogeneous catalyst of Fenton reaction for degradation of Rhodamine B under visible light irradiation.

    Science.gov (United States)

    Lin, Kun-Yi Andrew; Lin, Jyun-Ting

    2017-09-01

    To enhance degradation of Rhodamine B (RhB), a toxic xanthene dye, an iron-doped graphitic carbon nitride (CN) is prepared by establishing a covalent bond (-CN-) bridging ferrocene (Fc) and CN via a Schiff base reaction. The π-conjugation between the aromatic Fc and CN can be much enhanced by the covalent bond, thereby facilitating the bulk-to-surface charge transfer and separation as well as reversible photo-redox reactions during photocatalytic reactions. Thus, the resulting Fc-CN exhibits a much higher catalytic activity than CN to activate hydrogen peroxide (HP) for RhB degradation, because the photocatalytically generated electrons from CN can activate HP and effectively maintain the bivalence state of Fe in Fc, which also induces the activation of HP. The RhB degradation by the Fc-CN activated HP process (Fc-CN-HP) is validated to involve OH • by examining the effect of radical probe agent as well as electron paramagnetic resonance (EPR) spectroscopic analysis. Fc-CN is also proven to activate HP for RhB degradation over multiple times without loss of catalytic activity. Through determining the degradation intermediates, RhB is indeed fully decomposed by Fc-CN-HP into much lower-molecular-weight organic compounds. These features indicate that Fc-functionalization can be an advantageous technique to enhance the catalytic activity of CN for activating HP. The results obtained in this study are essential to further design and utilize Fc-functionalized CN for Fenton-like reactions. The findings shown here, especially the degradation mechanism and pathway, are also quite important for treating xanthene dyes in wastewater. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Near-Infrared Fluorescence Detection of Acetylcholine in Aqueous Solution Using a Complex of Rhodamine 800 and p-Sulfonato-calix[8]arene

    Science.gov (United States)

    Jin, Takashi

    2010-01-01

    The complexing properties of p-sulfonatocalix[n]arenes (n = 4: S[4], n = 6: S[6], and n = 8: S[8]) for rhodamine 800 (Rh800) and indocyanine green (ICG) were examined to develop a near-infrared (NIR) fluorescence detection method for acetylcholine (ACh). We found that Rh800 (as a cation) forms an inclusion complex with S[n], while ICG (as a twitter ion) have no binding ability for S[n]. The binding ability of Rh800 to S[n] decreased in the order of S[8] > S[6] >> S[4]. By the formation of the complex between Rh800 and S[8], fluorescence intensity of the Rh800 was significantly decreased. From the fluorescence titration of Rh800 by S[8], stoichiometry of the Rh800-S[8] complex was determined to be 1:1 with a dissociation constant of 2.2 μM in PBS. The addition of ACh to the aqueous solution of the Rh800-S[8] complex caused a fluorescence increase of Rh800, resulting from a competitive replacement of Rh800 by ACh in the complex. From the fluorescence change by the competitive fluorophore replacement, stoichiometry of the Rh800-ACh complex was found to be 1:1 with a dissociation constant of 1.7 mM. The effects of other neurotransmitters on the fluorescence spectra of the Rh800-S[8] complex were examined for dopamine, GABA, glycine, and l-asparatic acid. Among the neurotransmitters examined, fluorescence response of the Rh800-S[8] complex was highly specific to ACh. Rh800-S[8] complexes can be used as a NIR fluorescent probe for the detection of ACh (5 × 10−4−10−3 M) in PBS buffer (pH = 7.2). PMID:22294934

  17. Comparison and mechanism of photocatalytic activities of N-ZnO and N-ZrO2 for the degradation of rhodamine 6G.

    Science.gov (United States)

    Sudrajat, Hanggara; Babel, Sandhya

    2016-05-01

    N-doped ZnO (N-ZnO) and N-doped ZrO2 (N-ZrO2) are synthesized by novel, simple thermal decomposition methods. The catalysts are evaluated for the degradation of rhodamine 6G (R6G) under visible and UV light. N-ZnO exhibits higher dye degradation under both visible and UV light compared to N-ZrO2 due to possessing higher specific surface area, lower crystalline size, and lower band gap. However, it is less reusable than N-ZrO2 and its photocatalytic activity is also deteriorated at low pH. At the same intensity of 3.5 W/m(2), UVC light is shown to be a better UV source for N-ZnO, while UVA light is more suitable for N-ZrO2. At pH 7 with initial dye concentration of 10 mg/L, catalyst concentration of 1 g/L, and UVC light, 94.3 % of R6G is degraded by N-ZnO within 2 h. Using UVA light under identical experimental conditions, 93.5 % degradation of R6G is obtained by N-ZrO2. Moreover, the type of light source is found to determine the reactive species produced in the R6G degradation by N-ZnO and N-ZrO2. Less oxidative reactive species such as superoxide radical and singlet oxygen play a major role in the degradation of R6G under visible light. On the contrary, highly oxidative hydroxyl radicals are predominant under UVC light. Based on the kinetic study, the adsorption of R6G on the catalyst surface is found to be the controlling step.

  18. Coupling carbon nanotube film microextraction with desorption corona beam ionization for rapid analysis of Sudan dyes (I-IV) and Rhodamine B in chilli oil.

    Science.gov (United States)

    Chen, Di; Huang, Yun-Qing; He, Xiao-Mei; Shi, Zhi-Guo; Feng, Yu-Qi

    2015-03-07

    A rapid analysis method by coupling carbon nanotube film (CNTF) microextraction with desorption corona beam ionization (DCBI) was developed for the determination of Sudan dyes (I-IV) and Rhodamine B in chilli oil samples. Typically, CNTF was immersed into the diluted solution of chilli oil for extraction, which was then placed directly under the visible plasma beam tip of the DCBI source for desorption and ionization. Under optimized conditions, five dyes were simultaneously determined using this method. Results showed that the analytes were enriched by the CNTF through the π-π interactions, and the proposed method could significantly improve the sensitivities of these compounds, compared to the direct analysis by DCBI-MS/MS. The method with a linear range of 0.08-12.8 μg g(-1) and good linear relationships (R(2) > 0.93) in a multiple reaction monitoring (MRM) mode was developed. Satisfactory reproducibility was achieved. Relative standard deviations (RSDs) were less than 20.0%. The recoveries ranged from 80.0 to 110.0%, and the limits of detection (LODs) were in the range of 1.4-21 ng g(-1). Finally, the feasibility of the method was further exhibited by the determination of five illegal dyes in chilli powder. These results demonstrate that the proposed method consumes less time and solvent than conventional HPLC-based methods and avoids the contamination of chromatographic column and ion source from non-volatile oil. With the help of a 72-well shaker, multiple samples could be treated simultaneously, which ensures high throughput for the entire pretreatment process. In conclusion, it provides a rapid and high-throughput approach for the determination of such illicit additions in chilli products.

  19. A comparison study of rhodamine B photodegradation over nitrogen-doped lamellar niobic acid and titanic acid under visible-light irradiation.

    Science.gov (United States)

    Li, Xiukai; Kikugawa, Naoki; Ye, Jinhua

    2009-01-01

    A solid-state reaction method with urea as a nitrogen precursor was used to prepare nitrogen-doped lamellar niobic and titanic solid acids (i.e., HNb(3)O(8) and H(2)Ti(4)O(9)) with different acidities for visible-light photocatalysis. The photocatalytic activities of the nitrogen-doped solid acids were evaluated for rhodamine B (RhB) degradation and the results were compared with those obtained over the corresponding nitrogen-doped potassium salts. Techniques such as XRD, BET, SEM, X-ray photoelectron spectroscopy, and UV-visible diffuse reflectance spectroscopy were adopted to explore the nature of the materials as well as the characteristics of the doped nitrogen species. It was found that the intercalation of the urea precursor helped to stabilize the layered structures of both lamellar solid acids and enabled easier nitrogen doping. The effects of urea intercalation were more significant for the more acidic HNb(3)O(8) sample than for the less acidic H(2)Ti(4)O(9). Compared with the nitrogen-doped KNb(3)O(8) and K(2)Ti(4)O(9) samples, the nitrogen-doped HNb(3)O(8) and H(2)Ti(4)O(9) solid acids absorb more visible light and exhibit a superior activity for RhB photodegradation under visible-light irradiation. The nitrogen-doped HNb(3)O(8) sample performed the best among all the samples. The results of the current study suggest that the protonic acidity of the lamellar solid-acid sample is a key factor that influences nitrogen doping and the resultant visible-light photocatalysis.

  20. Growth temperature dependent surface plasmon resonances of densely packed gold nanoparticles’ films and their role in surface enhanced Raman scattering of Rhodamine6G

    International Nuclear Information System (INIS)

    Verma, Shweta; Rao, B. Tirumala; Bhartiya, S.; Sathe, V.; Kukreja, L.M.

    2015-01-01

    Highlights: • Growth temperature produces and tunes the surface plasmon resonance (SPR) of gold films. • Optimum thickness and growth temperature combination results narrow SPR band. • Alumina capping red-shifted the SPR band and showed marginal re-sputtering of films. • Densely packed gold nanoparticles of varying sizes can be realized by pulsed laser deposition. • High SERS intensity of dye from gold films of large SPR strength at excitation wavelength. - Abstract: Localized surface plasmon resonance (LSPR) characteristics of gold nanoparticles films grown at different substrate temperatures and mass thicknesses with and without alumina capping were studied. At different film mass thicknesses, the LSPR response was observed mainly in the films grown at high substrate temperatures. About 300 °C substrate temperature was found to be optimum for producing narrow and strong LSPR band in both uncapped and alumina capped gold nanoparticles films. The LSPR wavelength could be tuned in the range of 600–750 nm by changing either number of ablation pulses or decreasing target to substrate distance (TSD) and alumina layer capping. Though the alumina capping re-sputtered the gold films still these films exhibited stronger LSPR response compared to the uncapped films. Atomic force microscopic analysis revealed formation of densely packed nanoparticles films exhibiting strong LSPR response which is consistent with the package density of the nanoparticles predicted by the theoretical calculations. The average size of nanoparticles increased with substrate temperature, number of ablation pulses and decreasing the TSD. For the same mass thickness of gold films grown at different substrate temperatures the surface enhanced Raman scattering (SERS) intensity of Rhodamine6G dye was found to be significantly different which had direct correlation with the LSPR strength of the films at the excitation wavelength

  1. Removal of rhodamine B (a basic dye) and thoron (an acidic dye) from dilute aqueous solutions and wastewater simulants by ion flotation.

    Science.gov (United States)

    Shakir, Kamal; Elkafrawy, Ahmed Faouzy; Ghoneimy, Hussein Fouad; Elrab Beheir, Shokry Gad; Refaat, Mamdoh

    2010-03-01

    The present work deals with removal, by ion flotation, of two dyes: a basic dye (rhodamine B (RB)) and an acidic one (thoron (TH)) from dilute aqueous solutions and simulated wastewaters. These dyes are widely used for analytical and biological staining purposes. Besides, RB is commonly used in dyeing of various industrial products. Therefore, wastewaters emanating from chemical and radiochemical laboratories, and biomedical and biological research laboratories may be contaminated with RB and TH. Ion flotation of these dyes has been investigated over a wide range of pH using the anionic surfactant, sodium lauryl sulfate (NaLS) and the cationic surfactant, cetyltrimethylammonium bromide (CTAB) as collectors. Successful removals could be achieved for RB and TH with the anionic collector, NaLS, and the cationic collector, CTAB, respectively. In addition to the effects of pH and type of collector on the efficiency of removal of each dye, the effects of collector and dye concentrations, frother dosage, ionic strength, bubbling time period and presence of foreign salts were investigated and the optimal removal conditions have been established. Removals exceeding 99.5 % and 99.9% could be achieved for RB and TH, respectively. The results obtained are discussed with respect to dissociation of dye, type of collector, ionic strength and sign and magnitude of charge of added foreign ions. Kinetics of flotation were also studied. Further studies demonstrate that under optimum conditions the developed flotation processes can be applied for the treatment of dye-contaminated wastewaters simulated to those generated at dyeing industries and radiochemical laboratories. Copyright 2009 Elsevier Ltd. All rights reserved.

  2. Photocatalytic applications with CdS • block copolymer/exfoliated graphene nanoensembles: hydrogen generation and degradation of Rhodamine B

    International Nuclear Information System (INIS)

    Skaltsas, T; Karousis, N; Pispas, S; Tagmatarchis, N

    2014-01-01

    Amphiphilic block copolymer poly(isoprene-b-acrylic acid) (PI-b-PAA) was used to stabilize exfoliated graphene in water, allowing the immobilization of semiconductor CdS nanoparticles forming CdS • PI-b-PAA/graphene. Characterization using high-resolution transmission electron microscopy and energy-dispersive x-ray spectroscopy proved the success of the preparation method and revealed the presence of spherical CdS. Moreover, UV–Vis and photoluminescence assays suggested that electronic interactions within CdS • PI-b-PAA/graphene exist as evidenced by the significant quenching of the characteristic emission of CdS by exfoliated graphene. Photoillumination of CdS • PI-b-PAA/graphene, in the presence of ammonium formate as a quencher for the photogenerated holes, resulted in the generation of hydrogen by water splitting, monitored by the reduction of 4-nitroaniline to benzene-1,4-diamine (>80 ± 4% at 20 min; 100% at 24 min), much faster and more efficient compared to when reference CdS • PI-b-PAA was used as the photocatalyst (<30 ± 3% at 20 min; 100% at 240 min). Moreover, Rhodamine B was photocatalytically degraded by CdS • PI-b-PAA/graphene, with fast kinetics under visible light illumination in the presence of air. The enhancement of both photocatalytic processes by CdS • PI-b-PAA/graphene was rationalized in terms of effective separation of holes and electrons, contrary to reference CdS • PI-b-PAA, in which rapid recombination of the hole–electron pair is inevitable due to the absence of exfoliated graphene as a suitable electron acceptor. (paper)

  3. Imaging lysosomal highly reactive oxygen species and lighting up cancer cells and tumors enabled by a Si-rhodamine-based near-infrared fluorescent probe.

    Science.gov (United States)

    Zhang, Hongxing; Liu, Jing; Liu, Chenlu; Yu, Pengcheng; Sun, Minjia; Yan, Xiaohan; Guo, Jian-Ping; Guo, Wei

    2017-07-01

    Lysosomes have recently been regarded as the attractive pharmacological targets for selectively killing of cancer cells via lysosomal cell death (LCD) pathway that is closely associated with reactive oxygen species (ROS). However, the details on the ROS-induced LCD of cancer cells are still poorly understood, partially due to the absence of a lysosome-targetable, robust, and biocompatible imaging tool for ROS. In this work, we brought forward a Si-rhodamine-based fluorescent probe, named PSiR, which could selectively and sensitively image the pathologically more relavent highly reactive oxygen species (hROS: HClO, HO, and ONOO - ) in lysosomes of cancer cells. Compared with many of the existing hROS fluorescent probes, its superiorities are mainly embodied in the high stability against autoxidation and photoxidation, near-infrared exitation and emission, fast fluorescence off-on response, and specific lysosomal localization. Its practicality has been demonstrated by the real-time imaging of hROS generation in lysosomes of human non-small-cell lung cancer cells stimulated by anticancer drug β-lapachone. Moreover, the probe was sensitive enough for basal hROS in cancer cells, allowing its further imaging applications to discriminate not only cancer cells from normal cells, but also tumors from healthy tissues. Overall, our results strongly indicated that PSiR is a very promising imaging tool for the studies of ROS-related LCD of cancer cells, screening of new anticancer drugs, and early diagnosis of cancers. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. A new turn-on fluorimetric method for the rapid speciation of Cr(III)/Cr(VI) species in tea samples with rhodamine-based fluorescent reagent

    Science.gov (United States)

    Özyol, Esra; Saçmacı, Şerife; Saçmacı, Mustafa; Ülgen, Ahmet

    2018-02-01

    A new fluorimetric method with rhodamine-based fluorescent agent was developed for the rapid speciation of Cr(III)/Cr(VI) in tea, soil and water samples. The system, which utilizes a fluorescent reagent, was used for the first time after synthesis/characterization of 3‧,6‧-bis(diethylamino)-2-{[(1E)-(2,4-dimethoxyphenyl)methylene] amino}spiro[isoindole-1,9‧-xanthen]-3(2H)-one (BDAS). The reagent responds instantaneously at room temperature in a 1:1 stoichiometric manner to the amount of Cr(III). The selectivity of this system for Cr(III) over other metal ions is remarkably high, and its sensitivity is below 0.01 mg L- 1 in aqueous solutions which enables a simplification without any pretreatment of the real sample. The method has a wide linear range of 0.1-10 mg L- 1 and a detection limit of 0.15 μg L- 1 for Cr(III) while the relative standard deviation was 0.1% for 0.1 mg L- 1 Cr(III) concentration. The results of detection and recovery experiments for Cr(III) in tea, soil and water were satisfactory, indicating that the method has better feasibility and application potential in the routine determination and speciation of Cr(III)/Cr(VI). The results of analysis of the certified reference material (INCT-TL-1 tea sample and CWW-TM-D waste water) are in good agreement with the certified value.

  5. A novel piperazine-bis(rhodamine-B)-based chemosensor for highly sensitive and selective naked-eye detection of Cu{sup 2+} and its application as an INHIBIT logic device

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Zebin; Li, Haizhen; Guo, Dan; Liu, Yan; Tian, Zhang; Yan, Shiqiang, E-mail: yansq@lzu.edu.cn

    2015-11-15

    Abstact: We report the design and synthesis of a new piperazine-bis(rhodamine-B) (RB-P-RB)-based indicator for selective detection of Cu{sup 2+} ion. Optical sensing behavior toward various metal ions including alkali, alkaline earth and transition metal ions (Na{sup +}, K{sup +}, Ba{sup 2+}, Mg{sup 2+}, Ca{sup 2+}, Zn{sup 2+}, Cd{sup 2+}, Mn{sup 2+}, Co{sup 2+}, Ni{sup 2+}, Pb{sup 2+}, Cu{sup 2+}, Hg{sup 2+}, and Ag{sup +}) were investigated by UV–vis and fluorescence spectroscopy in ethassnol solution. The indicator showed highly selective and sensitive colorimetric and “turn-on” fluorescence enhancement responses toward Cu{sup 2+} ion owing to the ring-opening structure of the rhodamine spirolactam. The significant change from colorless to pink upon the addition of Cu{sup 2+} could make it a suitable “naked-eye” indicator for Cu{sup 2+}. Furthermore, a possible ring-opening mechanism (off-on) of the rhodamine spirolactam induced by Cu{sup 2+} binding is supported by Job plot, ESI-mass, FT-IR, and {sup 1}H NMR. More significantly, the probe displayed highly selective Cu{sup 2+}-amplified absorption in the presence of Cu{sup 2+} ions. Finally, using Cu{sup 2+} and EDTA as inputs and the fluorescence emission intensity as output, an INHIBIT logic gate can be constructed at the molecular level. - Highlights: • A novel piperazine-bis(rhodamine-B)-based sensor for selective detection of Cu{sup 2+} ion was synthesized via simple synthetic procedures. • The probe exhibited highly selective and sensitive colorimetric and “turn on” fluorescence enhancement responses to Cu{sup 2+}. • The probe can serve as a reversible and selective “naked eye” indicator for Cu{sup 2+} ions in ethanol solution. • The probe can be utilized to construct an INHIBIT logic gate at the molecular level. • The probe displays highly selective Cu{sup 2+}-amplified absorption in ethanol solution.

  6. Enhanced Sonocatalytic Degradation of Rhodamine B by Graphene-TiO2 Composites Synthesized by an Ultrasonic-Assisted Method%Enhanced Sonocatalytic Degradation of Rhodamine B by Graphene-TiO2 Composites Synthesized by an Ultrasonic-Assisted Method

    Institute of Scientific and Technical Information of China (English)

    ZHU Lei; Trisha GHOSH; Chong-Yeon PARK; MENG Ze-Da; OH Won-Chun

    2012-01-01

    A series of graphene-TiO2 composites was fabricated from graphene oxide and titanium n-butoxide (TNB) by an ultrasonic-assisted method.The structure and composition of the nanocomposites were characterized by Raman spectroscopy,BET surface area measurements,X-ray diffraction,transmission electron microscopy,and ultraviolet-visible absorption spectroscopy.The average size of the TiO2 nanoparticles on the graphene nanosheets was controlled at around 10-15 nm without using surfactant,which is attributed to the pyrolysis and condensation of dissolved TNB into TiO2 by ultrasonic irradiation.The catalytic activity of the composites under ultrasonic irradiation was determined using a rhodamine B (RhB) solution.The graphene-TiO2 composites possessed a high specific surface area,which increased the decolorization rate for RhB solution.This is because the graphene and TiO2 nanoparticles in the composites interact strongly,which enhances the photoelectric conversion of TiO2 by reducing the recombination ofphotogenerated electron-hole pairs.

  7. Hydrothermal synthesis of Ti oxide nanostructures and TiO{sub 2}:SnO{sub 2} heterostructures applied to the photodegradation of rhodamine B

    Energy Technology Data Exchange (ETDEWEB)

    Mourao, Henrique A.J.L., E-mail: henriquepiau@yahoo.com.br [Universidade Federal de Sao Carlos, Departamento de Quimica, Rod. Washington Luiz, km 235, CEP 13565-905, Sao Carlos, SP (Brazil); EMBRAPA Instrumentacao Agropecuaria, Rua XV de Novembro, 1452, CEP 13560-970, CP 741, Sao Carlos, SP (Brazil); Junior, Waldir Avansi; Ribeiro, Caue [EMBRAPA Instrumentacao Agropecuaria, Rua XV de Novembro, 1452, CEP 13560-970, CP 741, Sao Carlos, SP (Brazil)

    2012-08-15

    The present study describes the synthesis, characterization and testing of the photocatalytic potential of TiO{sub 2} nanoparticles (NPs), TiO{sub 2}:SnO{sub 2} heterostructures and potassium titanate nanotubes (TNTs) obtained by the alkaline hydrothermal method. The materials were characterized by X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy, surface area estimated from the N{sub 2} physisorption isotherm (BET), X-ray absorption near-edge structure (XANES) spectroscopy, scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), scanning transmission electron microscopy (STEM) and Fourier transform near-infrared (FT-NIR) spectroscopy, among other methods. Photocatalytic potential was assessed by rhodamine B dye photodegradation under UVC radiation. The properties of the materials were shown to depend on the KOH concentration. Potassium TNTs with high surface area were obtained only in 5 mol L{sup -1} KOH. The material composed of TiO{sub 2} anatase phase, which was obtained in KOH solution ranging from 10{sup -4} to 1 mol L{sup -1}, showed higher photocatalytic activity than the TNTs, despite the lower surface area and lower density of hydroxyl groups on the anatase. In the heterostructure syntheses, SnO{sub 2} NPs were identified attached to TiO{sub 2} when 10{sup -4} and 10{sup -2} mol L{sup -1} KOH were used, whereas at [KOH] = 1 and 5 mol L{sup -1}, Sn remained in solution during the synthetic process and only the respective TiO{sub 2} phase was identified. The TiO{sub 2}:SnO{sub 2} heterostructures were more active than the material without SnO{sub 2} prepared at the same KOH concentrations. Highlights: Black-Right-Pointing-Pointer The formation of the materials depends on the [KOH] used during syntheses. Black-Right-Pointing-Pointer The heterostructures were obtained with the lower [KOH]. Black-Right-Pointing-Pointer Photoactivity of the heterostructures was higher than the respective TiO{sub 2

  8. Significant visible-light photocatalytic enhancement in Rhodamine B degradation of silver orthophosphate via the hybridization of N-doped graphene and poly(3-hexylthiophene)

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yanlin, E-mail: zhangyl@scnu.edu.cn; Xie, Churu; Gu, Feng Long, E-mail: Gu@scnu.edu.cn; Wu, Honghai; Guo, Qiang

    2016-09-05

    Highlights: • The Ag{sub 3}PO{sub 4}/NG/P3HT composites were synthesized via a facile method. • The Ag{sub 3}PO{sub 4}/NG/P3HT composites exhibit excellent photocatalytic activity for RhB degradation. • The kinetic constant of Ag{sub 3}PO{sub 4}/NG/P3HT is more than 6 times of pristine Ag{sub 3}PO{sub 4}. • The composites show better recyclability and stability than pristine Ag{sub 3}PO{sub 4}. - Abstract: Organic pollutants as typical water contaminants are potentially harmful to human health. In this study, we suggested that the novel Ag{sub 3}PO{sub 4}/N-doped graphene (NG)/Poly(3-hexylthiophene) (P3HT) composites can remove the organic dye Rhodamine B (RhB) from water. This Ag{sub 3}PO{sub 4}-based photocatalyst was synthesized via a facile method and subsequently characterized by XRD, SEM, TEM, XPS, Raman spectroscopy, PL spectroscopy, and UV–vis DRS. The photocatalytic activity of Ag{sub 3}PO{sub 4}/NG/P3HT composites is significantly higher than that of pristine Ag{sub 3}PO{sub 4}, Ag{sub 3}PO{sub 4}/NG, and Ag{sub 3}PO{sub 4}/P3HT for RhB degradation under visible light irradiation, especially the kinetic constant of Ag{sub 3}PO{sub 4}/NG/P3HT is more than 6 times of pristine Ag{sub 3}PO{sub 4}. The reactive oxygen species trapping experiments indicate that the degradation of RhB over the Ag{sub 3}PO{sub 4}/NG/P3HT composites mainly results from the holes oxidation and superoxide radical reduction. Besides, Ag{sub 3}PO{sub 4}/NG/P3HT composites exhibit better recyclability and stability than pristine Ag{sub 3}PO{sub 4}. Furthermore, the photocatalytic mechanism of Ag{sub 3}PO{sub 4}/NG/P3HT composites for RhB degradation under visible light was proposed as the synergistic effect of irradiated Ag{sub 3}PO{sub 4}, P3HT and NG sheets on the effective separation of photogenerated electron-hole pairs, and the enhancement of visible light absorbance.

  9. Hydrothermal synthesis of Ti oxide nanostructures and TiO2:SnO2 heterostructures applied to the photodegradation of rhodamine B

    International Nuclear Information System (INIS)

    Mourão, Henrique A.J.L.; Junior, Waldir Avansi; Ribeiro, Caue

    2012-01-01

    The present study describes the synthesis, characterization and testing of the photocatalytic potential of TiO 2 nanoparticles (NPs), TiO 2 :SnO 2 heterostructures and potassium titanate nanotubes (TNTs) obtained by the alkaline hydrothermal method. The materials were characterized by X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy, surface area estimated from the N 2 physisorption isotherm (BET), X-ray absorption near-edge structure (XANES) spectroscopy, scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), scanning transmission electron microscopy (STEM) and Fourier transform near-infrared (FT-NIR) spectroscopy, among other methods. Photocatalytic potential was assessed by rhodamine B dye photodegradation under UVC radiation. The properties of the materials were shown to depend on the KOH concentration. Potassium TNTs with high surface area were obtained only in 5 mol L −1 KOH. The material composed of TiO 2 anatase phase, which was obtained in KOH solution ranging from 10 −4 to 1 mol L −1 , showed higher photocatalytic activity than the TNTs, despite the lower surface area and lower density of hydroxyl groups on the anatase. In the heterostructure syntheses, SnO 2 NPs were identified attached to TiO 2 when 10 −4 and 10 −2 mol L −1 KOH were used, whereas at [KOH] = 1 and 5 mol L −1 , Sn remained in solution during the synthetic process and only the respective TiO 2 phase was identified. The TiO 2 :SnO 2 heterostructures were more active than the material without SnO 2 prepared at the same KOH concentrations. Highlights: ► The formation of the materials depends on the [KOH] used during syntheses. ► The heterostructures were obtained with the lower [KOH]. ► Photoactivity of the heterostructures was higher than the respective TiO 2 nanostructures. ► Titanate nanotubes showed high concentration of OH groups but low photoactivity.

  10. Effects of the iron oxide nanoparticle Molday ION Rhodamine B on the viability and regenerative function of neural stem cells: relevance to clinical translation

    Directory of Open Access Journals (Sweden)

    Umashankar A

    2016-04-01

    Full Text Available Abhishek Umashankar,1,2,* Mandi J Corenblum,1,* Sneha Ray,1,2,* Michel Valdez,3 Eriko S Yoshimaru,3 Theodore P Trouard,3,4 Lalitha Madhavan1,4 1Department of Neurology, 2Neuroscience and Cognitive Science Undergraduate Program, Undergraduate Biology Research Program, 3Department of Biomedical Engineering, 4Evelyn F McKnight Brain Institute, University of Arizona, Tucson, AZ, USA *These authors contributed equally to this work Abstract: An essential component of developing successful neural stem cell (NSC-based therapies involves the establishment of methodologies to noninvasively monitor grafted NSCs within brain tissues in real time. In this context, ex vivo labeling with ultrasmall superparamagnetic iron oxide (USPIO particles has been shown to enable efficient tracking of transplanted NSCs via magnetic resonance imaging (MRI. However, whether and how USPIO labeling affects the intrinsic biology of NSCs is not thoroughly understood, and remains an active area of investigation. Here, we perform a comprehensive examination of rat NSC survival and regenerative function upon labeling with the USPIO, Molday ION Rhodamine B (MIRB, which allows for dual magnetic resonance and optical imaging. After optimization of labeling efficiency, two specific doses of MIRB (20 and 50 µg/mL were chosen and were followed for the rest of the study. We observed that both MIRB doses supported the robust detection of NSCs, over an extended period of time in vitro and in vivo after transplantation into the striata of host rats, using MRI and post hoc fluorescence imaging. Both in culture and after neural transplantation, the higher 50 µg/mL MIRB dose significantly reduced the survival, proliferation, and differentiation rate of the NSCs. Interestingly, although the lower 20 µg/mL MIRB labeling did not produce overtly negative effects, it increased the proliferation and glial differentiation of the NSCs. Additionally, application of this dose also changed the

  11. Effects of the iron oxide nanoparticle Molday ION Rhodamine B on the viability and regenerative function of neural stem cells: relevance to clinical translation.

    Science.gov (United States)

    Umashankar, Abhishek; Corenblum, Mandi J; Ray, Sneha; Valdez, Michel; Yoshimaru, Eriko S; Trouard, Theodore P; Madhavan, Lalitha

    2016-01-01

    An essential component of developing successful neural stem cell (NSC)-based therapies involves the establishment of methodologies to noninvasively monitor grafted NSCs within brain tissues in real time. In this context, ex vivo labeling with ultrasmall superparamagnetic iron oxide (USPIO) particles has been shown to enable efficient tracking of transplanted NSCs via magnetic resonance imaging (MRI). However, whether and how USPIO labeling affects the intrinsic biology of NSCs is not thoroughly understood, and remains an active area of investigation. Here, we perform a comprehensive examination of rat NSC survival and regenerative function upon labeling with the USPIO, Molday ION Rhodamine B (MIRB), which allows for dual magnetic resonance and optical imaging. After optimization of labeling efficiency, two specific doses of MIRB (20 and 50 μg/mL) were chosen and were followed for the rest of the study. We observed that both MIRB doses supported the robust detection of NSCs, over an extended period of time in vitro and in vivo after transplantation into the striata of host rats, using MRI and post hoc fluorescence imaging. Both in culture and after neural transplantation, the higher 50 μg/mL MIRB dose significantly reduced the survival, proliferation, and differentiation rate of the NSCs. Interestingly, although the lower 20 μg/mL MIRB labeling did not produce overtly negative effects, it increased the proliferation and glial differentiation of the NSCs. Additionally, application of this dose also changed the morphological characteristics of neurons and glia produced after NSC differentiation. Importantly, the transplantation of NSCs labeled with either of the two MIRB doses upregulated the immune response in recipient animals. In particular, in animals receiving the 50 μg/mL MIRB-labeled NSCs, this immune response consisted of an increased number of CD68(+)-activated microglia, which appeared to have phagocytosed MIRB particles and cells contributing to an

  12. Enhanced visible-light photocatalytic activity of Fe/ZnO for rhodamine B degradation and its photogenerated charge transfer properties

    International Nuclear Information System (INIS)

    Yi, Shasha; Cui, Jiabao; Li, Shuo; Zhang, Lijing; Wang, Dejun; Lin, Yanhong

    2014-01-01

    Highlights: • ZnO and Fe/ZnO nanoflowers were synthesized via a hydrothermal process. • Fe/ZnO nanoflowers show improved photocatalytic activity under the irradiation of visible light. • In the Fe/ZnO system, Fe 3+ and Fe 2+ coexistences in the ZnO host. • The detailed photocatalytic mechanism and the role of Fe in the photodegradation system are discussed. - Abstract: Zinc oxide (ZnO) and iron doped zinc oxide (Fe/ZnO) nanoflowers were successfully synthesized via a simple hydrothermal process. The samples were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman scattering, ultraviolet–visible (UV–vis) diffuse spectroscopy and scanning electron microscopy (SEM), and it was found that the dopant ions replaced some of the crystal lattice zinc ions, and furthermore, Fe 3+ and Fe 2+ ions coexist. Photocatalytic activities of the catalysts were assessed based on the degradation of rhodamine B (RhB) in aqueous solution under both UV and visible light irradiation (λ > 420 nm), respectively. All Fe/ZnO samples showed enhanced photocatalytic activity under the irradiation of visible light. On the contrary, Fe/ZnO products displayed poorer performance than that of pure ZnO in the presence of UV light. This phenomenon can be attributed to the coexistence of Fe 3+ and Fe 2+ in the ZnO host. The photophysical mechanism of the UV and visible photocatalytic activity was investigated with the help of surface photovoltage (SPV) and PL measurements, respectively. The results indicated the influence of coexistence of Fe 3+ and Fe 2+ in ZnO host on the separation and transfer behavior of the photogenerated charges in the UV and visible regions, which are distinctly different: under the irradiation of UV light, the recombination of the photogenerated electrons and holes was promoted, whereas the separation and transfer of photogenerated electrons and holes was facilitated under the visible light irradiation. The detailed photocatalytic

  13. Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications.

    Science.gov (United States)

    Belov, Vladimir N; Mitronova, Gyuzel Yu; Bossi, Mariano L; Boyarskiy, Vadim P; Hebisch, Elke; Geisler, Claudia; Kolmakov, Kirill; Wurm, Christian A; Willig, Katrin I; Hell, Stefan W

    2014-10-06

    Caged rhodamine dyes (Rhodamines NN) of five basic colors were synthesized and used as "hidden" markers in subdiffractional and conventional light microscopy. These masked fluorophores with a 2-diazo-1-indanone group can be irreversibly photoactivated, either by irradiation with UV- or violet light (one-photon process), or by exposure to intense red light (λ∼750 nm; two-photon mode). All dyes possess a very small 2-diazoketone caging group incorporated into the 2-diazo-1-indanone residue with a quaternary carbon atom (C-3) and a spiro-9H-xanthene fragment. Initially they are non-colored (pale yellow), non-fluorescent, and absorb at λ=330-350 nm (molar extinction coefficient (ε)≈10(4)  M(-1)  cm(-1)) with a band edge that extends to about λ=440 nm. The absorption and emission bands of the uncaged derivatives are tunable over a wide range (λ=511-633 and 525-653 nm, respectively). The unmasked dyes are highly colored and fluorescent (ε=3-8×10(4)  M(-1)  cm(-1) and fluorescence quantum yields (ϕ)=40-85% in the unbound state and in methanol). By stepwise and orthogonal protection of carboxylic and sulfonic acid groups a highly water-soluble caged red-emitting dye with two sulfonic acid residues was prepared. Rhodamines NN were decorated with amino-reactive N-hydroxysuccinimidyl ester groups, applied in aqueous buffers, easily conjugated with proteins, and readily photoactivated (uncaged) with λ=375-420 nm light or intense red light (λ=775 nm). Protein conjugates with optimal degrees of labeling (3-6) were prepared and uncaged with λ=405 nm light in aqueous buffer solutions (ϕ=20-38%). The photochemical cleavage of the masking group generates only molecular nitrogen. Some 10-40% of the non-fluorescent (dark) byproducts are also formed. However, they have low absorbance and do not quench the fluorescence of the uncaged dyes. Photoactivation of the individual molecules of Rhodamines NN (e.g., due to reversible or irreversible

  14. Pd-MnO2 nanoparticles/TiO2 nanotube arrays (NTAs) photo-electrodes photo-catalytic properties and their ability of degrading Rhodamine B under visible light.

    Science.gov (United States)

    Thabit, Mohamed; Liu, Huiling; Zhang, Jian; Wang, Bing

    2017-10-01

    Pd-MnO 2 /TiO 2 nanotube arrays (NTAs) photo-electrodes were successfully fabricated via anodization and electro deposition subsequently; the obtained Pd-MnO 2 /TiO 2 NTAs photo electrodes were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD) and characterized accordingly. Moreover, the light harvesting and absorption properties were investigated via ultraviolet-visible diffuse reflectance spectrum (DRS); photo degradation efficiency was investigated via analyzing the photo catalytic degradation of Rhodamine B under visible illumination (xenon light). The performed analyses illustrated that Pd-MnO 2 codoped particles were successfully deposited onto the surface of the TiO 2 nanotube arrays; DRS results showed significant improvement in visible light absorption which was between 400 and 700nm. Finally, the photo catalytic degradation efficiency results of the designated organic pollutant (Rhodamine B) illustrated a superior photocatalytic (PC) efficiency of approximately 95% compared to the bare TiO 2 NTAs, which only exhibited a photo catalytic degradation efficiency of approximately 61%, thus it indicated the significant enhancement of the light absorption properties of fabricated photo electrodes and their yield of OH radicals. Copyright © 2017. Published by Elsevier B.V.

  15. α-Amino Acid Derived Benzimidazole-Linked Rhodamines: A Case of Substitution Effect at the Amino Acid Site toward Spiro Ring Opening for Selective Sensing of Al3+ Ions.

    Science.gov (United States)

    Majumdar, Anupam; Mondal, Subhendu; Daniliuc, Constantin G; Sahu, Debashis; Ganguly, Bishwajit; Ghosh, Sourav; Ghosh, Utpal; Ghosh, Kumaresh

    2017-08-07

    α-Amino acid derived benzimidazole-linked rhodamines have been synthesized, and their metal ion sensing properties have been evaluated. Experimentally, l-valine- and l-phenylglycine-derived benzimidazole-based rhodamines 1 and 2 selectively recognize Al 3+ ion in aqueous CH 3 CN (CH 3 CN/H 2 O 4/1 v/v, 10 mM tris HCl buffer, pH 7.0) over the other cations by exhibiting color and "turn-on" emission changes. In contrast, glycine-derived benzimidazole 3 remains silent in the recognition event and emphasizes the role of α-substitution of amino acid undertaken in the design. The fact has been addressed on the basis of the single-crystal X-ray structures and theoretical calculations. Moreover, pink 1·Al 3+ and 2·Al 3+ ensembles selectively sensed F - ions over other halides through a discharge of color. Importantly, compounds 1 and 2 are cell permeable and have been used as imaging reagents for the detection of Al 3+ uptake in human lung carcinoma cell line A549.

  16. Synthesis, characterization of organo-modified zeolitic nanomaterial from coal ash and application as adsorbent on remediation of contaminated water by rhodamine B and direct blue 71

    International Nuclear Information System (INIS)

    Alcântara, Raquel Reis

    2016-01-01

    The synthesis of zeolites from mineral coal fly and bottom ash was performed by alkaline hydrothermal treatment, which were named ZFA and ZBA, respectively. Organo-modified zeolites, SMZF and SMZB, were obtained from surface modification of ZFA and ZBA, respectively, using the cationic surfactant hexadecyltrimethylammonium bromide. From the remaining solutions generated in ZFA and ZBA synthesis it was possible to synthesis two new zeolites. The physicochemical characteristics of the synthesized nanomaterials zeolite as well as their respective raw materials, such as cation exchange capacity, density, specific area, chemical composition, mineralogical and morphological, among others, were determined. The adsorbents SMZF and SMZB were used to remove the dyes, Direct Blue 71 (DB71) and Rhodamine B (RB) from aqueous solutions in batch system. Thus, four systems DB71/SMZF, RB/SMZF, DB71/SMZB, RB/SMZB were investigated. The models of pseudo-first order and pseudo-second order were applied to the experimental data for the study the adsorption kinetics. The model of pseudo-second order was the one that best described the adsorption of all dye/organomodified-zeolites systems. The equilibrium adsorption was analyzed from four models isotherm, namely: Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D-B). The results show that the model Freundlich and Langmuir best described the experimental data systems DB71/SMZF and DB71/SMZB, respectively. For systems with RB, the model D-R was the best fit for both adsorbents (SMZF and SMZB). The factorial design 2 4 was applied to the analysis of the following factors influencing the adsorption process: initial concentration of dye (C o ), pH, amount of adsorbent (M) and temperature (T). Under the conditions studied it concludes with the confidence interval of 95%, which for the DB71/SMZF system, the factors and their interactions that influence more were C 0 , M, pH, pH∗M, pH∗C 0 , M∗C 0 , pH∗M∗C 0 , in that order. In DB

  17. Synthesis and evaluation of a new Rhodamine B and Di(2-picolyl)amine conjugate as a highly sensitive and selective chemosensor for Al3+ and its application in living-cell imaging.

    Science.gov (United States)

    Bao, Xiaofeng; Cao, Qiansheng; Xu, Yazhou; Gao, Yuanxue; Xu, Yuan; Nie, Xuemei; Zhou, Baojing; Pang, Tao; Zhu, Jing

    2015-02-15

    A new Rhodamine B derivative (RBDPA), namely, N(1)-(2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)ethyl)-N(4),N(4)-bis(pyridin-2-ylmethyl)succinamide, was designed, synthesized and structurally characterized to develop a chemosensor. The studies show that RBDPA exhibits high sensitivity and selectivity toward Al(3+) among many other metal cations in an ethanol/H2O (1:1, v/v, pH=7.2, HEPES buffer, 0.1mM) solution. Fluorescence microscopy experiments further demonstrate that RBDPA can be used as a fluorescent probe to detect Al(3+) in living cells. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Polyaniline nanotubes coated with TiO2&γ-Fe2O3@graphene oxide as a novel and effective visible light photocatalyst for removal of rhodamine B from water

    Science.gov (United States)

    Ghavami, Monireh; Kassaee, Mohammad Zaman; Mohammadi, Reza; Koohi, Maryam; Haerizadeh, Bibi Narjes

    2014-12-01

    Synthesis of polyaniline-nanotubes (PANI-NT), in the presence of TiO2 and γ-Fe2O3 functionalized graphene oxide (GO), gives a green and magnetically recyclable photocatalyst, TiO2&γ-Fe2O3@GO/PANI-NT. The later orchestrates 94% photocatalytic efficiency in removal of rhodamine B (RB) from water, under simulated solar light irradiation. This is far higher than the 36% observed in the presence of TiO2&γ-Fe2O3@GO alone, where PANI-NT is excluded from the structure. Morphology, composition, and structural properties of our economically sound photocatalyst are characterized by X-ray diffraction, energy-dispersive X-ray spectroscopy, thermo-gravimetric, transmission electron microscopy, inductively coupled plasma, RAMAN and Fourier-transform infrared spectroscopy.

  19. SIRB, sans iron oxide rhodamine B, a novel cross-linked dextran nanoparticle, labels human neuroprogenitor and SH-SY5Y neuroblastoma cells and serves as a USPIO cell labeling control.

    Science.gov (United States)

    Shen, Wei-Bin; Vaccaro, Dennis E; Fishman, Paul S; Groman, Ernest V; Yarowsky, Paul

    2016-05-01

    This is the first report of the synthesis of a new nanoparticle, sans iron oxide rhodamine B (SIRB), an example of a new class of nanoparticles. SIRB is designed to provide all of the cell labeling properties of the ultrasmall superparamagnetic iron oxide (USPIO) nanoparticle Molday ION Rhodamine B (MIRB) without containing the iron oxide core. MIRB was developed to label cells and allow them to be tracked by MRI or to be manipulated by magnetic gradients. SIRB possesses a similar size, charge and cross-linked dextran coating as MIRB. Of great interest is understanding the biological and physiological changes in cells after they are labeled with a USPIO. Whether these effects are due to the iron oxide buried within the nanoparticle or to the surface coating surrounding the iron oxide core has not been considered previously. MIRB and SIRB represent an ideal pairing of nanoparticles to identify nanoparticle anatomy responsible for post-labeling cytotoxicity. Here we report the effects of SIRB labeling on the SH-SY5Y neuroblastoma cell line and primary human neuroprogenitor cells (hNPCs). These effects are contrasted with the effects of labeling SH-SY5Y cells and hNPCs with MIRB. We find that SIRB labeling, like MIRB labeling, (i) occurs without the use of transfection reagents, (ii) is packaged within lysosomes distributed within cell cytoplasm, (iii) is retained within cells with no loss of label after cell storage, and (iv) does not alter cellular viability or proliferation, and (v) SIRB labeled hNPCs differentiate normally into neurons or astrocytes. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  20. An enhanced cerium(IV)-rhodamine 6G chemiluminescence system using guest-host interactions in a lab-on-a-chip platform for estimating the total phenolic content in food samples.

    Science.gov (United States)

    Al Haddabi, Buthaina; Al Lawati, Haider A J; Suliman, FakhrEldin O

    2016-04-01

    Two chemiluminescence-microfluidic (CL-MF) systems, e.g., Ce(IV)-rhodamine B (RB) and Ce(IV)-rhodamine 6G (R6G), for the determination of the total phenolic content in teas and some sweeteners were evaluated. The results indicated that the Ce(IV)-R6G system was more sensitive in comparison to the Ce(IV)-RB CL system. Therefore, a simple (CL-MF) method based on the CL of Ce(IV)-R6G was developed, and the sensitivity, selectivity and stability of this system were evaluated. Selected phenolic compounds (PCs), such as quercetin (QRC), catechin (CAT), rutin (RUT), gallic acid (GA), caffeic acid (CA) and syringic acid (SA), produced analytically useful chemiluminescence signals with low detection limits ranging from 0.35 nmol L(-1) for QRC to 11.31 nmol L(-1) for SA. The mixing sequence and the chip design were crucial, as the sensitivity and reproducibility could be substantially affected by these two factors. In addition, the anionic surfactant (i.e., sodium dodecyl sulfate (SDS)) can significantly enhance the CL signal intensity by as much as 300% for the QRC solution. Spectroscopic studies indicated that the enhancement was due to a strong guest-host interaction between the cationic R6G molecules and the anionic amphiphilic environment. Other parameters that could affect the CL intensities of the PCs were carefully optimized. Finally, the method was successfully applied to tea and sweetener samples. Six different tea samples exhibited total phenolic/antioxidant levels from 7.32 to 13.5 g per 100g of sample with respect to GA. Four different sweetener samples were also analyzed and exhibited total phenolic/antioxidant levels from 500.9 to 3422.9 mg kg(-1) with respect to GA. The method was selective, rapid and sensitive when used to estimate the total phenolic/antioxidant level, and the results were in good agreement with those reported for honey and tea samples. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. A highly sensitive fluorescence quenching method for perphenazine detection based on its catalysis of K{sub 2}S{sub 2}O{sub 8} oxidizing rhodamine 6G

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Lihong; Huang, Qitong; Lin, Changqing [Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou, 363000 (China); Lin, Xiaofeng [College of Chemistry and Environment, Minnan Normal University, Zhangzhou, 363000 (China); Huang, Yiqun [Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000 (China); Liu, Jiaming, E-mail: mnsdljm@163.com [College of Chemistry and Environment, Minnan Normal University, Zhangzhou, 363000 (China); Ma, Xudong, E-mail: maxudong005@hotmail.com [Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000 (China)

    2014-12-15

    In this paper, the fluorescence spectra of Rhod 6G (rhodamine 6G)–K{sub 2}S{sub 2}O{sub 8}–PPH (perphenazine) were studied. We found that Rhod 6G existed in the form of Rhod 6G{sup +} under the conditions of 60 °C, 10 min and pH 5.42, and Rhod 6G{sup +} can emit strong and stable fluorescence. Further study showed that when PPH and Rhod 6G{sup +} coexisted, the ester exchange reaction carried out between -OH of PPH and -COOC{sub 2}H{sub 5} of Rhod 6G{sup +} to produced Rhod 6G{sup +}–PPH compound. More interestingly, K{sub 2}S{sub 2}O{sub 8} could oxidize Rhod 6G{sup +} and quench its RTP signal, while PPH was oxidized to red compound PPH′ by K{sub 2}S{sub 2}O{sub 8}, and Rhod 6G{sup +}–PPH′ and PPH were produced in the ester exchange reaction between the -OH of PPH′ and the -COOC{sub 2}H{sub 5} of Rhod 6G{sup +}–PPH. In the above process, PPH catalyzed K{sub 2}S{sub 2}O{sub 8} oxidizing Rhod 6G, which caused the fluorescence signal of the system to quench sharply. Hence, a catalytic fluorescence quenching method for the determination of residual PPH has been developed based on the its catalyzing K{sub 2}S{sub 2}O{sub 8} oxidize rhodamine 6G. This sensitive, accurate, simple and selective fluorescence quenching method was used to determine residual PPH in biological samples with the results consisting with those obtained by high performance liquid chromatography (HPLC), showing good accuracy. The structures of Rhod 6G{sup +}, PPH and Rhod 6G{sup +}–PPH were characterized by infrared spectra. The reaction mechanism of the determination of PPH was also discussed. - Highlights: • Fluorescence for the determination of perphenazine (PPH) had been established. • This method had high sensitivity (limit of detection was 3.3×10{sup −14} g mL{sup −1}). • This method had been applied to determination of PPH in biological samples. • Structures of Rhod 6G{sup +}, PPH and Rhod 6G{sup +}–PPH were characterized by infrared spectra. • Mechanism

  2. Synthesis and Characterization of Reduced Graphene Oxide/Rhodamine 101 (rGO-Rh101) Nanocomposites and Their Heterojunction Performance in rGO-Rh101/ p-Si Device Configuration

    Science.gov (United States)

    Batır, G. Güven; Arık, Mustafa; Caldıran, Zakir; Turut, Abdulmecit; Aydogan, Sakir

    2018-01-01

    Reduced graphene oxide (rGO)-rhodamine 101 (Rh101) nanocomposites with different ratios of rGO have been synthesized in aqueous medium by ultrasonic homogenization. The fluorescence of Rh101 as measured using a laser dye with high fluorescence quantum yield was substantially quenched with increasing amount of rGO in the nanocomposite. Formation of rGO-Rh101 nanocomposites was confirmed by x-ray diffraction analysis, scanning electron microscopy, ultraviolet-visible (UV-Vis) spectroscopy, and fluorescence microscopy. Furthermore, rGO-Rh101 nanocomposite/ p-Si heterojunctions were synthesized, all of which showed good rectifying behavior. The electrical characteristics of these devices were analyzed using current-voltage ( I- V) measurements to determine the ideality factor and barrier height. The experimental results confirmed the presence of lateral inhomogeneity in the effective barrier height of the rGO-Rh101 nanocomposite/ p-Si heterojunctions. In addition to I- V measurements, one device was analyzed in more detail using frequency-dependent capacitance-voltage measurements. All electrical measurements were carried out at room temperature and in the dark.

  3. Effective degradation of rhodamine B by electro-Fenton process, using ferromagnetic nanoparticles loaded on modified graphite felt electrode as reusable catalyst: in neutral pH condition and without external aeration.

    Science.gov (United States)

    Tian, Jiangnan; Zhao, Jixiang; Olajuyin, Ayobami Matthew; Sharshar, Moustafa Mohamed; Mu, Tingzhen; Yang, Maohua; Xing, Jianmin

    2016-08-01

    Polytetrafluoroethylene/ferromagnetic nanoparticle/carbon black (PTFE/MNP/CB)-modified graphite felt (GF) was successfully applied as cathode for the mineralization of rhodamine B (RhB) in electro-Fenton (EF) process. The modified cathode showed high decolorization efficiency for RhB solution even in neutral pH condition and without external aeration, achieving nearly complete decolorization and 89.52 % total organic carbon (TOC) removal after 270-min oxidation with the MNP load 1.2 g at 50 A/m(2). Moreover, the operational parameters (current density, MNP load, initial pH, and airflow rate) were optimized. After that, adsorption isotherm was also conducted to compare the absorption quantity of CB and carbon nanotube (CNT). Then, the surface morphologies of MNPs were characterized by transmission electron microscope (TEM), energy-dispersive X-ray detector (EDX), and Fourier transform infrared spectroscopy (FTIR); and the modified cathode was characterized by SEM and contact angle. Finally, the stability and reusability of modified cathode were tested. Result uncovered that the PTFE/MNP/CB-modified cathode has the potential for industrial application and the solution after treatment was easily biodegradable.

  4. Effects of hepatic ischemia-reperfusion injury on the P-glycoprotein activity at the liver canalicular membrane and blood-brain barrier determined by in vivo administration of rhodamine 123 in rats.

    Science.gov (United States)

    Miah, Mohammad K; Shaik, Imam H; Bickel, Ulrich; Mehvar, Reza

    2014-04-01

    To investigate the effects of normothermic hepatic ischemia-reperfusion (IR) injury on the activity of P-glycoprotein (P-gp) in the liver and at the blood-brain barrier (BBB) of rats using rhodamine 123 (RH-123) as an in vivo marker. Rats were subjected to 90 min of partial ischemia or sham surgery, followed by 12 or 24 h of reperfusion. Following intravenous injection, the concentrations of RH-123 in blood, bile, brain, and liver were used for pharmacokinetic calculations. The protein levels of P-gp and some other transporters in the liver and brain were also determined by Western blot analysis. P-gp protein levels at the liver canalicular membrane were increased by twofold after 24 h of reperfusion. However, the biliary excretion of RH-123 was reduced in these rats by 26%, presumably due to IR-induced reductions in the liver uptake of the marker and hepatic ATP concentrations. At the BBB, a 24% overexpression of P-gp in the 24-h IR animals was associated with a 30% decrease in the apparent brain uptake clearance of RH-123. The pharmacokinetics or brain distribution of RH-123 was not affected by the 12-h IR injury. Hepatic IR injury may alter the peripheral pharmacokinetics and brain distribution of drugs that are transported by P-gp and possibly other transporters.

  5. Layer-by-Layer Self-Assembled Metal-Ion- (Ag-, Co-, Ni-, and Pd- Doped TiO2 Nanoparticles: Synthesis, Characterisation, and Visible Light Degradation of Rhodamine B

    Directory of Open Access Journals (Sweden)

    Mphilisi M. Mahlambi

    2012-01-01

    Full Text Available Metal-ion- (Ag, Co, Ni and Pd doped titania nanocatalysts were successfully deposited on glass slides by layer-by-layer (LbL self-assembly technique using a poly(styrene sulfonate sodium salt (PSS and poly(allylamine hydrochloride (PAH polyelectrolyte system. Solid diffuse reflectance (SDR studies showed a linear increase in absorbance at 416 nm with increase in the number of m-TiO2 thin films. The LbL assembled thin films were tested for their photocatalytic activity through the degradation of Rhodamine B under visible-light illumination. From the scanning electron microscope (SEM, the thin films had a porous morphology and the atomic force microscope (AFM studies showed “rough” surfaces. The porous and rough surface morphology resulted in high surface areas hence the high photocatalytic degradation (up to 97% over a 6.5 h irradiation period using visible-light observed. Increasing the number of multilayers deposited on the glass slides resulted in increased film thickness and an increased rate of photodegradation due to increase in the availability of more nanocatalysts (more sites for photodegradation. The LbL assembled thin films had strong adhesion properties which made them highly stable thus displaying the same efficiencies after five (5 reusability cycles.

  6. An efficient and ultrasensitive rhodamine B-based reversible colorimetric chemosensor for naked-eye recognition of molybdenum and citrate ions in aqueous solution: sensing behavior and logic operation.

    Science.gov (United States)

    Tavallali, Hossein; Deilamy-Rad, Gohar; Parhami, Abolfath; Hasanli, Nahid

    2015-03-15

    In this paper we manifest a novel rhodamine B (RhB) based colorimetric chemosensor for molybdenum and citrate ions (Cit(3-)) in an absolutely aqueous media. It has been identified as highly sensitive probe for Mo(6+) which responds at 4.0 nmol L(-1) concentration levels. RhB while combined with Mo(6+) in aqueous solution displays a color changing from pink to purple which could be quickly dissociated by the addition of citrate in this system so that reversible color changes from purple to pink can be achieved. The comparison of this method with some other methods for citrate indicates that this is the only method which can detect citrate in aqueous solution by color changes. This chemosensor can be applied for quantification of citrate with a linear range covering from 1.67×10(-7) to 1.22×10(-5) M and a detection limit of 2.0×10(-8) M. Moreover, the response of the chemosensor toward Mo(6+) and citrate is fast. In addition, based on above sensing mechanism, an IMPLICATION logic operation can be achieved using Mo(6+) ion and Cit(3-) as the inputs, making RhB a promising candidate for further applications in molecular logic devices and also indicates that RhB is suitable for the detection of Mo(6+) and Cit(3-) ions in real samples. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. Pd-catalytic in situ generation of H2O2 from H2 and O2 produced by water electrolysis for the efficient electro-fenton degradation of rhodamine B.

    Science.gov (United States)

    Yuan, Songhu; Fan, Ye; Zhang, Yucheng; Tong, Man; Liao, Peng

    2011-10-01

    A novel electro-Fenton process was developed for wastewater treatment using a modified divided electrolytic system in which H2O2 was generated in situ from electro-generated H2 and O2 in the presence of Pd/C catalyst. Appropriate pH conditions were obtained by the excessive H+ produced at the anode. The performance of the novel process was assessed by Rhodamine B (RhB) degradation in an aqueous solution. Experimental results showed that the accumulation of H2O2 occurred when the pH decreased and time elapsed. The maximum concentration of H2O2 reached 53.1 mg/L within 120 min at pH 2 and a current of 100 mA. Upon the formation of the Fenton reagent by the addition of Fe2+, RhB degraded completely within 30 min at pH 2 with a pseudo first order rate constant of 0.109 ± 0.009 min(-1). An insignificant decline in H2O2 generation and RhB degradation was found after six repetitions. RhB degradation was achieved by the chemisorption of H2O2 on the Pd/C surface, which subsequently decomposed into •OH upon catalysis by Pd0 and Fe2+. The catalytic decomposition of H2O2 to •OH by Fe2+ was more powerful than that by Pd0, which was responsible for the high efficiency of this novel electro-Fenton process.

  8. Optimizing the Removal of Rhodamine B in Aqueous Solutions by Reduced Graphene Oxide-Supported Nanoscale Zerovalent Iron (nZVI/rGO Using an Artificial Neural Network-Genetic Algorithm (ANN-GA

    Directory of Open Access Journals (Sweden)

    Xuedan Shi

    2017-06-01

    Full Text Available Rhodamine B (Rh B is a toxic dye that is harmful to the environment, humans, and animals, and thus the discharge of Rh B wastewater has become a critical concern. In the present study, reduced graphene oxide-supported nanoscale zero-valent iron (nZVI/rGO was used to treat Rh B aqueous solutions. The nZVI/rGO composites were synthesized with the chemical deposition method and were characterized using scanning electron microscopy (SEM, X-ray diffraction (XRD, Raman spectroscopy, N2-sorption, and X-ray photoelectron spectroscopy (XPS analysis. The effects of several important parameters (initial pH, initial concentration, temperature, and contact time on the removal of Rh B by nZVI/rGO were optimized by response surface methodology (RSM and artificial neural network hybridized with genetic algorithm (ANN-GA. The results suggest that the ANN-GA model was more accurate than the RSM model. The predicted optimum value of Rh B removal efficiency (90.0% was determined using the ANN-GA model, which was compatible with the experimental value (86.4%. Moreover, the Langmuir, Freundlich, and Temkin isotherm equations were applied to fit the adsorption equilibrium data, and the Freundlich isotherm was the most suitable model for describing the process for sorption of Rh B onto the nZVI/rGO composites. The maximum adsorption capacity based on the Langmuir isotherm was 87.72 mg/g. The removal process of Rh B could be completed within 20 min, which was well described by the pseudo-second order kinetic model.

  9. Synthesis and characterization of novel PPy/Bi{sub 2}O{sub 2}CO{sub 3} composite with improved photocatalytic activity for degradation of Rhodamine-B

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Qizhao, E-mail: wangqizhao@163.com [College of Chemistry and Chemical Engineering, Northwest Normal University, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education of China, Key Laboratory of Gansu Polymer Materials, Lanzhou 730070 (China); Zheng, Longhui; Chen, Yutao; Fan, Jiafeng [College of Chemistry and Chemical Engineering, Northwest Normal University, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education of China, Key Laboratory of Gansu Polymer Materials, Lanzhou 730070 (China); Huang, Haohao, E-mail: scuthhh@hotmail.com [College of Materials Science and Engineering, South China University of Technology, Guangzhou 510640 (China); Su, Bitao [College of Chemistry and Chemical Engineering, Northwest Normal University, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education of China, Key Laboratory of Gansu Polymer Materials, Lanzhou 730070 (China)

    2015-07-15

    Highlights: • A new photocatalyst PPy/Bi{sub 2}O{sub 2}CO{sub 3} was synthesized by a simple hydrothermal method. • The PPy/Bi{sub 2}O{sub 2}CO{sub 3} photocatalyst shows enhanced degradation activity of RhB under UV light irradiation. • A photocatalytic mechanism is proposed based on the synergistic effect of PPy and Bi{sub 2}O{sub 2}CO{sub 3}. - Abstract: Photocatalyst Bi{sub 2}O{sub 2}CO{sub 3} modified by polypyrrole (PPy) was synthesized via a facile hydrothermal method. As-prepared PPy/Bi{sub 2}O{sub 2}CO{sub 3} composites were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and UV–vis diffuse reflectance spectroscopy (DRS). Presence of PPy did not affect the crystal structure, but exerted great influence on the photocatalytic activity of Bi{sub 2}O{sub 2}CO{sub 3} and enhanced absorption band of pure Bi{sub 2}O{sub 2}CO{sub 3}. The photocatalytic activities of the PPy/Bi{sub 2}O{sub 2}CO{sub 3} samples were determined by photocatalytic degradation of Rhodamine-B (RhB) under ultra violet (UV) irradiation and 0.75 wt.% PPy/Bi{sub 2}O{sub 2}CO{sub 3} composite showed the highest photocatalytic activity. The enhanced photocatalytic performance could be attributed to the synergistic effect of PPy and Bi{sub 2}O{sub 2}CO{sub 3}. A possible photocatalytic mechanism of the PPy/Bi{sub 2}O{sub 2}CO{sub 3} photocatalysts was proposed in order to guide the further improvement of its photocatalytic performance.

  10. One pot hydrothermal synthesis of a novel BiIO4/Bi2MoO6 heterojunction photocatalyst with enhanced visible-light-driven photocatalytic activity for rhodamine B degradation and photocurrent generation

    International Nuclear Information System (INIS)

    Huang, Hongwei; Liu, Liyuan; Zhang, Yihe; Tian, Na

    2015-01-01

    Graphical abstract: The efficient charge transfer occurred at the interface of BiIO 4 /Bi 2 MoO 6 heterojunction results in the efficient separation of photoexcited electron–hole pairs and promotes the photocatalytic activity. - Highlights: • BiIO 4 /Bi 2 MoO 6 composites were synthesized by a one-step hydrothermal method. • The BiIO 4 /Bi 2 MoO 6 composite exhibits much better photoelectrochemical performance. • The highly improved photocatalytic activity is attributed to heterojunction structure. • Holes (h + ) are the main active species in the photodegradation process of RhB. - Abstract: A novel BiIO 4 /Bi 2 MoO 6 heterojunction photocatalyst has been successfully developed by a one-step hydrothermal method for the first time. It was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and diffuse reflection spectroscopy (DRS). Compared to pure BiIO 4 and Bi 2 MoO 6 , the BiIO 4 /Bi 2 MoO 6 composite exhibits the much better photoelectrochemical performance for Rhodamine B (RhB) degradation and photocurrent (PC) generation under visible light irradiation (λ > 420 nm). This enhancement on visible-light-responsive photocatalytic activity should be attributed to the fabrication of a BiIO 4 /Bi 2 MoO 6 heterojunction, thus resulting in the high separation and transfer efficiency of photogenerated charge carriers. The supposed photocatalytic mechanism dominated by holes (h + ) was verified by the photoluminescence (PL) spectroscopy, electrochemical impedance spectra (EIS) and active species trapping experiments

  11. Fabrication of flower-like direct Z-scheme β-Bi2O3/g-C3N4 photocatalyst with enhanced visible light photoactivity for Rhodamine B degradation

    Science.gov (United States)

    Zhang, Liping; Wang, Guohong; Xiong, Zhenzhong; Tang, Hua; Jiang, Chuanjia

    2018-04-01

    A combined hydrothermal-calcination approach is developed to synthesize hierarchical β-Bi2O3/g-C3N4 direct Z-scheme photocatalyst with enhanced visible light photoactivity for Rhodamine B (RhB) degradation. First, Bi2O2CO3 microflowers were hydrothermally prepared using Bi(NO3)3·5H2O as feedstocks, and then a series of β-Bi2O3/g-C3N4 direct Z-scheme photocatalysts were synthesized via a facile calcination method using Bi2O2CO3 and g-C3N4 as precursors. The samples were systematically characterized by various characterization technologies including X-ray diffraction, scanning and transmission electron microscopes, Fourier transform infrared spectroscopy and N2 absorption-desorption equipment. It was found that the g-C3N4 content in the precursors played a key role in affecting the photocatalytic activity of the final products. The β-Bi2O3/g-C3N4 heterojunction exhibited higher photocatalytic activity than single active components (β-Bi2O3 and g-C3N4), indicating the presence of a synergistic effect between two active components in β-Bi2O3/g-C3N4 heterojunction. Among all as-prepared catalysts, the 70 wt.% g-C3N4/Bi2O2CO3 exhibits the highest activity for RhB degradation, and the apparent reaction rate constant k (42.2 × 10-3 min-1) is 3.1 and 1.7 times as high as that of pure β-Bi2O3 (13.5 × 10-3 min-1) and g-C3N4 (25.2 × 10-3 min-1), respectively. The enhanced photocatalytic performance of β-Bi2O3/g-C3N4 heterostructure photocatalysts is mainly due to the high surface area, closely contacted interfaces between the β-Bi2O3 and g-C3N4 component, and the formation of direct Z-scheme structure in the β-Bi2O3/g-C3N4 composites.

  12. An ultrasensitive and highly selective fluorescent and colorimetric chemosensor for citrate ions based on rhodamine B and its application as the first molecular security keypad lock based on phosphomolybdic acid and citrate inputs

    Energy Technology Data Exchange (ETDEWEB)

    Tavallali, Hossein, E-mail: Tavallali@pnu.ac.ir; Baezzat, Mohammad-Reza; Deilamy-Rad, Gohar; Parhami, Abolfath; Hasanli, Nahid

    2015-04-15

    Rhodamine B (Rh{sub B}) has been developed as novel and efficient colorimetric and fluorometric chemosensor for citrate ions (Cit{sup 3−}) in an absolutely aqueous media. The UV–vis absorption and fluorescent emission titrations experiments have been employed to study the sensing process. Rh{sub B} could act as an efficient “ON–OFF” fluorescent chemosensor for phosphomolybdic acid (PMA) based on an electron transfer (ET) process. Also (Rh{sub B}{sup +}){sub 3}.PMA{sup 3−} could operate as an “OFF–ON” fluorescent chemosensor for citrate ions based on a ligand substitution process. The chemosensor Rh{sub B} shows excellent fluorescence sensitivity and selectivity toward citrate in aqueous media, and displays ON–OFF–ON type fluorescence change with alternately adding PMA and citrate to the media along with reversible association–dissociation of the complex. The (Rh{sub B}{sup +}){sub 3}.PMA{sup 3−} can be applied to the quantification of citrate with a linear ranges covering from 0.053 to 0.83 and 0.08 to 1.6 µM by detection limits of 6.0 and 9.1 nM for fluorescence and colorimetric methods respectively. The keypad lock operation is particularly important, as the output of the system depends not only on the proper combination but also on the order of input signals, creating the correct password that can be used to “open” this molecular keypad lock through strong fluorescence emission at 575 nm. As a whole, its various logic gate properties may improve its impact for the development of new-generation “intelligence” digital devices. The ionic PMA and Cit{sup 3−} inputs to (Rh{sub B}{sup +}){sub 3}.PMA{sup 3−} have been mimicked as a superimposed electronic molecular keypad lock. Also indicates that Rh{sub B} is suitable for the detection of Cit{sup 3−} ions in the biological environment. - Highlights: • Our probe is commercially available with good photostability and high quantum yield. • Both color and fluorescence change

  13. 软模板法制备中孔碳材料及其对罗丹明B吸附性能的研究%Synthesis of mesoporous carbon by soft-templating method and it absorption performance for Rhodamine B

    Institute of Scientific and Technical Information of China (English)

    陈刚; 赵振波; 杜鹏

    2012-01-01

    Mesoporous carbon has been synthesized by soft-templating method. The samples were characterized by XRD,FT-IR,SEM and N2 adsorption-desorption method. The adsorption properties of mesoporous carbon to Rhodamine B were investigated detailedly. It was found that adsorption capacity of mesoporous carbon depends on pH of solution, temperature and mass of adsorbent. When the pH value and temperature were 1 and 25 % respectively, the adsorption properties of mesoporous carbon to Rhodamine B are the best. ;%采用软模板法合成了较大比表面积的中孔碳材料,通过XRD、FT-IR、SEM、N2吸附-脱附对样品进行了表征.并以所制备的中孔碳材料为吸附剂,以罗丹明B溶液模拟染料废水,进行了吸附脱色实验,考察了吸附时的pH值、温度、吸附剂加入量等因素对吸附性能的影响.研究发现在pH=1,吸附温度为25℃时,所合成的中孔碳材料对罗丹明B表现出了最好的吸附性能.

  14. In situ hydrothermal synthesis of g-C{sub 3}N{sub 4}/TiO{sub 2} heterojunction photocatalysts with high specific surface area for Rhodamine B degradation

    Energy Technology Data Exchange (ETDEWEB)

    Hao, Ruirui [Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, Institute for Advanced Materials, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002 (China); Wang, Guohong, E-mail: wanggh2003@163.com [Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, Institute for Advanced Materials, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002 (China); Jiang, Chuanjia [State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Luoshi Road 122, Wuhan, 430070 (China); Tang, Hua [School of Materials Science and Engineering, Jiangsu University, Zhenjiang, 212013 (China); Xu, Qingchuan [Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, Institute for Advanced Materials, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002 (China)

    2017-07-31

    Highlights: • High surface area g-C{sub 3}N4/TiO{sub 2} is obtained by combined hydrothermal-calcination method. • HCl formation from Ti precursor changed the structural properties of the composite. • Composite exhibited visible light photocatalytic performance for RhB degradation. • A 3 g of melamine for 0.5 mL TiCl{sub 4} was found to be the optimum content. - Abstract: Semiconductor-based photocatalysis is a promising method for degradation of environmental pollutants, but the activity of most widely used photocatalysts such as titania (TiO{sub 2}) is still unsatisfactory under visible light. Herein, we synthesized a highly efficient visible-light-responsive heterojunction catalysts based on graphitic carbon nitride (g-C{sub 3}N{sub 4}) and TiO{sub 2}. The g-C{sub 3}N{sub 4}/TiO{sub 2} heterojunction composites with high specific surface area were prepared via in situ hydrothermal synthesis followed by calcination, using titanium tetrachloride (TiCl{sub 4}) and melamine as precursors. Interesting, HCl from the hydrolysis of TiCl{sub 4} served as the proton source to acidify the melamine. The g-C{sub 3}N{sub 4}/TiO{sub 2} heterojunction composites exhibited higher photocatalytic performance for decomposition of Rhodamine B (RhB) than pure g-C{sub 3}N{sub 4} or TiO{sub 2} under visible light irradiation. The high activity can be ascribed to the high specific surface area (up to 115.6 m{sup 2} g{sup −1}) of the g-C{sub 3}N{sub 4}/TiO{sub 2} composites and a synergistic heterojunction structure between TiO{sub 2} and g-C{sub 3}N{sub 4}. Moreover, the photocatalytic performances of the g-C{sub 3}N{sub 4}/TiO{sub 2} composites rely on the content of melamine in the synthesis precursors: with an optimum melamine content (3 g for 0.5 mL of TiCl{sub 4}), the sample showed the highest photocatalytic performance, which is superior to pure TiO{sub 2} and g-C{sub 3}N{sub 4} by a factor of 18.7 and 3.5, respectively. Active species trapping experiments revealed that

  15. Excitonic Behavior of Rhodamine Dimers: A Single-Molecule Study

    NARCIS (Netherlands)

    Hernando Campos, J.; van der Schaaf, Martijn; van Dijk, E.M.H.P.; Sauer, Markus; Garcia Parajo, M.F.; van Hulst, N.F.

    2003-01-01

    The optical behavior of a dimer of tetramethylrhodamine-5-isothiocyanate has been investigated by means of single-molecule measurements. Bulk absorption and fluorescence spectra show the existence of two populations of the dimer molecule that exhibit distinct excitonic interactions (strong and weak

  16. Thermodynamics of the Rhodamine B Lactone--Zwitterion Equilibrium.

    Science.gov (United States)

    Hinckley, Daniel A.; Seybold, Paul G.

    1987-01-01

    Discusses the benefits of thermochromic transformations for studying thermodynamic properties. Describes an experiment that uses a commercially available dye, attains equilibrium rapidly, employs a simple, single-beam spectrophotometer, and is suitable for both physical chemistry and introductory chemistry laboratories. (TW)

  17. Synthesis and characterization of a novel rhodamine labeled cholesterol reporter.

    Science.gov (United States)

    Maiwald, Alexander; Bauer, Olivia; Gimpl, Gerald

    2017-06-01

    We introduce the novel fluorescent cholesterol probe RChol in which a sulforhodamine group is linked to the sixth carbon atom of the steroid backbone of cholesterol. The same position has recently been selected to generate the fluorescent reporter 6-dansyl-cholestanol (DChol) and the photoreactive 6-azi-cholestanol. In comparison with DChol, RChol is brighter, much more photostable, and requires less energy for excitation, i.e. favorable conditions for microscopical imaging. RChol easily incorporates into methyl-β-cyclodextrin forming a water-soluble inclusion complex that acts as an efficient sterol donor for cells and membranes. Like cholesterol, RChol possesses a free 3'OH group, a prerequisite to undergo intracellular esterification. RChol was also able to support the growth of cholesterol auxotrophic cells and can therefore substitute for cholesterol as a major component of the plasma membrane. According to subcellular fractionation, slight amounts of RChol (~12%) were determined in low-density Triton-insoluble fractions whereas the majority of RChol was localized in non-rafts fractions. In phase-separated giant unilamellar vesicles, RChol preferentially partitions in liquid-disordered membrane domains. Intracellular RChol was transferred to extracellular sterol acceptors such as high density lipoproteins in a dose-dependent manner. Unlike DChol, RChol was not delivered to the cholesterol storage pathway. Instead, it translocated to endosomes/lysosomes with some transient contacts to peroxisomes. Thus, RChol is considered as a useful probe to study the endosomal/lysosomal pathway of cholesterol. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. A new method for dosing rhodamine B in natural water

    International Nuclear Information System (INIS)

    Marichal, M.; Benoit, R.

    1961-01-01

    A simple and sensitive method well adapted to hydrological research. The dye is first extracted from the water sample by isoamyl alcohol and then the fluorescence of the alcoholic solution, after excitation by ultraviolet radiation, is measured spectrophotometrically. The sensitivity of the method is about 10 -12 , that is, a millionth of a milligram of dye per litre. Reprint of a paper published in 'Chimie Analytique', N. 2, Feb 1962, p. 70-72 [fr

  19. Adsorption of Rhodamine B Dye from Aqueous Solution on Irvingia ...

    African Journals Online (AJOL)

    NICOLAAS

    second-order kinetics model was observed to fit the adsorption data. Solution pH ... usually released containing various dyes and heavy metals.1 In particular ...... B.S. Ogunsina, O.A. Koya and O.O. Adeosun, Deformation and frac- ture of dika ...

  20. A new method for dosing rhodamine B in natural water; Dosage de la rhodamine B dans les eaux naturelles

    Energy Technology Data Exchange (ETDEWEB)

    Marichal, M.; Benoit, R. [Commissariat a l' energie atomique et aux energies alternatives - CEA, Centre de Marcoule, Centre de Production de Plutonium de Marcoule, Service de Protection contre les Radiations (France)

    1961-07-01

    A simple and sensitive method well adapted to hydrological research. The dye is first extracted from the water sample by isoamyl alcohol and then the fluorescence of the alcoholic solution, after excitation by ultraviolet radiation, is measured spectrophotometrically. The sensitivity of the method is about 10{sup -12}, that is, a millionth of a milligram of dye per litre. Reprint of a paper published in 'Chimie Analytique', N. 2, Feb 1962, p. 70-72 [French] Methode simple et sensible convenant bien aux recherches hydrologiques. Le colorant est prealablement extrait de l'echantillon d'eau par l'alcool isoamylique; la fluorescence de la solution alcoolique eclairee en lumiere ultraviolette est ensuite mesuree au spectrophotometre. La sensibilite de la methode est de l'ordre de I0{sup -12}, soit un millionieme de milligramme de colorant par litre. Reproduction d'un article publie dans 'Chimie Analytique', N. 2, Feb 1962, p. 70-72.

  1. Synthesis, characterization of organo-modified zeolitic nanomaterial from coal ash and application as adsorbent on remediation of contaminated water by rhodamine B and direct blue 71; Síntese, caracterização de nanomaterial zeolítico de cinzas de carvão organomodificado e aplicação como adsorvente na remediação de água contaminada por Rodamina B e Azul Direto 71

    Energy Technology Data Exchange (ETDEWEB)

    Alcântara, Raquel Reis

    2016-07-01

    The synthesis of zeolites from mineral coal fly and bottom ash was performed by alkaline hydrothermal treatment, which were named ZFA and ZBA, respectively. Organo-modified zeolites, SMZF and SMZB, were obtained from surface modification of ZFA and ZBA, respectively, using the cationic surfactant hexadecyltrimethylammonium bromide. From the remaining solutions generated in ZFA and ZBA synthesis it was possible to synthesis two new zeolites. The physicochemical characteristics of the synthesized nanomaterials zeolite as well as their respective raw materials, such as cation exchange capacity, density, specific area, chemical composition, mineralogical and morphological, among others, were determined. The adsorbents SMZF and SMZB were used to remove the dyes, Direct Blue 71 (DB71) and Rhodamine B (RB) from aqueous solutions in batch system. Thus, four systems DB71/SMZF, RB/SMZF, DB71/SMZB, RB/SMZB were investigated. The models of pseudo-first order and pseudo-second order were applied to the experimental data for the study the adsorption kinetics. The model of pseudo-second order was the one that best described the adsorption of all dye/organomodified-zeolites systems. The equilibrium adsorption was analyzed from four models isotherm, namely: Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D-B). The results show that the model Freundlich and Langmuir best described the experimental data systems DB71/SMZF and DB71/SMZB, respectively. For systems with RB, the model D-R was the best fit for both adsorbents (SMZF and SMZB). The factorial design 2{sup 4} was applied to the analysis of the following factors influencing the adsorption process: initial concentration of dye (C{sub o}), pH, amount of adsorbent (M) and temperature (T). Under the conditions studied it concludes with the confidence interval of 95%, which for the DB71/SMZF system, the factors and their interactions that influence more were C{sub 0}, M, pH, pH∗M, pH∗C{sub 0}, M∗C{sub 0}, p

  2. Sensing of polymeric sensor-based rhodamine B derivative for metal ...

    Indian Academy of Sciences (India)

    The development of fluorescent sensors for the detection of environmentally ... mechanical and thermal stability, weak chemical union with the metals ..... signals corresponding to the vinyl groups of the monomer. (d(H) 5.77 .... Photographs recorded under (a) visible light and (b) UV light at 365 nm for aqueous solutions of ...

  3. A new rhodamine based 'turn-on' ion selective chemosensor in ...

    Indian Academy of Sciences (India)

    ABHISHEK MAJI

    2017-08-03

    Aug 3, 2017 ... working under a physiological condition with a fast response is highly .... fluorescence microscope (Carl Zeiss, Germany) using an oil immersion lens at 63X ... mg/mL PBS) was added to each well of a 96-well culture plate and ...

  4. Biodistribution of rhodamine B fluorescence-labeled cationic nanoparticles in rats

    DEFF Research Database (Denmark)

    Knudsen, K. B.; Northeved, H.; Gjetting, Torben

    2014-01-01

    We investigated the biodistribution following the administration of nanosized (about 50 and 90 nm) cationic (ζ: +30 and +50 mV) micelles and liposomes intended for drug delivery. The particles were stable and well characterized with respect to size and ζ potential. Ten 5- to 6-week-old male rats ...

  5. Quenching of photoluminescence of Rhodamine 6G molecules on functionalized graphene

    Czech Academy of Sciences Publication Activity Database

    Valeš, Václav; Kovaříček, Petr; Ji, X.; Ling, X.; Kong, J.; Dresselhaus, M. S.; Kalbáč, Martin

    2016-01-01

    Roč. 253, č. 12 (2016), s. 2347-2350 ISSN 0370-1972 Institutional support: RVO:61388955 Keywords : atomic force microscopy * functionalized graphene * photoluminiscence quenching Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.674, year: 2016

  6. Tip Enhanced Raman Spectroscopy of Rhodamine 6G on nanostructured gold substrate

    KAUST Repository

    Moretti, Manola

    2015-05-01

    A new concept based setup for Tip Enhanced Raman Scattering measurement assisted by gold nanostructure is presented, that can provide a platform for gap-mode enhancement of the signal at the single molecule level conjugated with controlled spatial localization of the molecule under investigation and a method to determine the diffraction limit properties of the tip. In essence, this effect is obtained illuminating a gold coated AFM tip which is raster scanned over a nanostructured gold substrate, after chemisorption of a Raman active molecule. We expect that the near-field Raman enhancement would be given by the gap-mode effect of the two facing nano-features. Thanks to the nanostructured substrate, we verify that the resolution of the Raman mapping signal is well below the diffraction limit given by the combination of the optics geometry and the laser wavelength. We show that the gap-mode TERS can generate an estimated field- enhancement (g) of ~20 in localized areas of the sample and we demonstrate the ability to spatially define the molecule position (by Raman mapping) at the tens of nanometers scale. © 2015 Elsevier Ltd.

  7. Sensing of polymeric sensor-based rhodamine B derivative for metal ...

    Indian Academy of Sciences (India)

    TONG-MOU GENG1 XIE WANG1 FENG ZHU1 HUI JIANG1 YU WANG2. Collaborative Innovation Center for Petrochemical New Materials, Anhui Key Laboratory of Functional Coordination Compounds, School of Chemistry and Chemical Engineering, Anqing Normal University, Anqing 246011, People's Republic of China ...

  8. Visualizing tributyltin (TBT) in bacterial aggregates by specific rhodamine-based fluorescent probes.

    Science.gov (United States)

    Jin, Xilang; Hao, Likai; She, Mengyao; Obst, Martin; Kappler, Andreas; Yin, Bing; Liu, Ping; Li, Jianli; Wang, Lanying; Shi, Zhen

    2015-01-01

    Here we present the first examples of fluorescent and colorimetric probes for microscopic TBT imaging. The fluorescent probes are highly selective and sensitive to TBT and have successfully been applied for imaging of TBT in bacterial Rhodobacter ferrooxidans sp. strain SW2 cell-EPS-mineral aggregates and in cell suspensions of the marine cyanobacterium Synechococcus PCC 7002 by using confocal laser scanning microscopy. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. Efficient tetracycline adsorption and photocatalytic degradation of rhodamine B by uranyl coordination polymer

    Science.gov (United States)

    Ren, Ya-Nan; Xu, Wei; Zhou, Lin-Xia; Zheng, Yue-Qing

    2017-07-01

    Two mixed uranyl-cadmium malonate coordination polymers [(UO2)2Cd(H-bipy)2(mal)4(H2O)2]·4H2O 1 and [(UO2)Cd(bipy)(mal)2]·H2O 2 (H2mal = malonic acid, bipy =4,4‧-bipyridine) have been synthesized in room temperature. Compound 1 represents a one-dimensional (1D) chain assembly of Cd(II) ions, uranyl centers and malonate ligands. Compound 2 exhibits a two-dimensional (2D) 2D +2D → 3D polycatenated framework based on inclined interlocked 2D 44 sql grids. The two compounds have been characterized by elemental analysis, IR and UV-vis spectroscopy, thermal analysis, powder X-ray diffraction and photoluminescence spectroscopy. And the ferroelectric property of 2 also has been studied. Moreover, compound 2 exhibits good photocatalytic activity for dye degradation under UV light and is excellent adsorbent for removing tetracycline antibiotics in the aqueous solution.

  10. Selective floatation-spectrophotometric determination of tellurium (4) with papaverine and butyl rhodamine B

    International Nuclear Information System (INIS)

    Skripchuk, V.G.

    1981-01-01

    It is shown, that papaverine reacts with a bromide complex of tellurium (4) to form a compound readily floated by toluene. The floatation is carried out from an aqueous solution, 5.2 M in H 2 SO 4 , 0.2 M in KBr and 5.4x10 -3 M in papaverine. The absorbance is a function of tellurium (4) concentration over a range of 5-100 μg Te/5 ml. Such a highly sensitive reagent as butylrhodamine B can be effectively substituted for papaverine. The floatation results in better selectivity. The method makes it possible to determine tellurium in blister, anodic and cathodic copper without matrix preseparation [ru

  11. Tip Enhanced Raman Spectroscopy of Rhodamine 6G on nanostructured gold substrate

    KAUST Repository

    Moretti, Manola; Das, Gobind; Torre, Bruno; Allione, Marco; Di Fabrizio, Enzo M.

    2015-01-01

    A new concept based setup for Tip Enhanced Raman Scattering measurement assisted by gold nanostructure is presented, that can provide a platform for gap-mode enhancement of the signal at the single molecule level conjugated with controlled spatial localization of the molecule under investigation and a method to determine the diffraction limit properties of the tip. In essence, this effect is obtained illuminating a gold coated AFM tip which is raster scanned over a nanostructured gold substrate, after chemisorption of a Raman active molecule. We expect that the near-field Raman enhancement would be given by the gap-mode effect of the two facing nano-features. Thanks to the nanostructured substrate, we verify that the resolution of the Raman mapping signal is well below the diffraction limit given by the combination of the optics geometry and the laser wavelength. We show that the gap-mode TERS can generate an estimated field- enhancement (g) of ~20 in localized areas of the sample and we demonstrate the ability to spatially define the molecule position (by Raman mapping) at the tens of nanometers scale. © 2015 Elsevier Ltd.

  12. AgBr/diatomite for the efficient visible-light-driven photocatalytic degradation of Rhodamine B

    Science.gov (United States)

    Fang, Jing; Zhao, Huamei; Liu, Qinglei; Zhang, Wang; Gu, Jiajun; Su, Yishi; Abbas, Waseem; Su, Huilan; You, Zhengwei; Zhang, Di

    2018-03-01

    The treatment of organic pollution via photocatalysis has been investigated for a few decades. However, earth-abundant, cheap, stable, and efficient substrates are still to be developed. Here, we prepare an efficient visible-light-driven photocatalyst via the deposition of Ag nanoparticles (light intensity. For comparison, AgBr/SiO2 ( κ = 0.04 min-1) and commercial AgBr nanoparticles ( κ = 0.05 min-1) were measured as well. The experimental results reveal that diatomite acted more than a substrate benefiting the dispersion of AgBr nanoparticles, as well as a cooperator to help harvest visible light and adsorb dye molecules, leading to the efficient visible-light-driven photocatalytic performance of AgBr/diatomite. Considering the low cost (10 per ton) and large-scale availability of diatomite, our study provides the possibility to prepare other types of diatomite-based efficient photocatalytic composites with low-cost but excellent photocatalytic performance.

  13. Rhodamine B removal on A-rGO/cobalt oxide nanoparticles composite by adsorption from contaminated water

    Science.gov (United States)

    Alwan, Salam H.; Alshamsi, Hassan A. Habeeb; Jasim, Layth S.

    2018-06-01

    Cobalt oxide nanoparticles@rGO composite is prepared by using graphene oxide (GO) as a supporting substance. GO is first treated with ascorbic acid to form rGO. Finally, cobalt oxide nanoparticles reaction with rGO sheets and using as the adsorbent to removal Rh.B dye from wastewater. The morphology and chemical structure of prepared samples were characterized by FTIR, X-ray spectroscopy, SEM-EDX, TEM, AFM and TGA. The adsorption of Rh.B dye on the A-rGO/Co3O4 composite was accomplished under different conditions that are equilibrium time, pH solution, ionic strength, and temperature. The adsorption isotherms of Rh.B dye on the A-rGO/Co3O4 composite could be illustrated well by the Langmuir, Freundlich and Tempkin model. The thermodynamic factors (ΔHo, ΔSo, and ΔGo) estimated from the temperature-dependent isotherms revealed that the adsorption reaction of Rh.B dye on the A-rGO/Co3O4 composite was an endothermic and spontaneous process.

  14. PEMANFAATAN GELOMBANG MIKRO DALAM EKSTRAKSI ANTOSIANIN DARI KULIT BUAH NAGA SEBAGAI PENGGANTI RHODAMIN B UNTUK SEDIAAN PEWARNA BIBIR ALAMI

    Directory of Open Access Journals (Sweden)

    Arief Adhiksana

    2017-06-01

    Full Text Available Today, microwave can be used in various chemical processes. The advantage of microwaves is able to deliver energy directly to the material. One of microwave application is the extraction process of natural materials. Microwaves can be used to reduce extraction process time of the anthocyanin from the dragon fruit skin. This research aims to determine the solvent extracted effect to the anthocyanin extraction of dragon fruit skin using microwaves. The solvent used was 95% ethanol, 85% ethanol, 75% ethanol, 95% ethanol –citric acid, 85% ethanol -acids and 75% - citric acid ethanol to the anthocyanin extraction results of dragon fruit skin extracted using microwaves. The consumption of microwave power is 400 watt with 50 gram of dragon fruits skin. Then, anthocyanin extracts are mixe with supporting materials to be made as lipstick and proposed for topical and favorite test. The best yield can be obtained by using ethanol-citric acid solvent at 25,031%.

  15. Shape-controlled synthesis of Sn-doped CuO nanoparticles for catalytic degradation of Rhodamine B

    Czech Academy of Sciences Publication Activity Database

    Vomáčka, Petr; Štengl, Václav; Henych, Jiří; Kormunda, M.

    2016-01-01

    Roč. 481, NOV (2016), s. 28-38 ISSN 0021-9797 R&D Projects: GA MŠk(CZ) LM2015073 Institutional support: RVO:61388980 Keywords : Sn-doped CuO * Tin doping * Copper oxide * Catalyst * Catalytic activity * Morphology Subject RIV: CA - Inorganic Chemistry Impact factor: 4.233, year: 2016

  16. THE APPLICATION OF NATURAL ZEOLITE FROM CIAMIS AS TiO2 PHOTOCATALYST SUPPORT FOR RHODAMINE B DYE PHOTODEGRADATION

    Directory of Open Access Journals (Sweden)

    Intan Cahaya Dani

    2016-04-01

    Full Text Available Heavy metals such as nickel and cadmium from the waste of human activities (industry, domestic, can lead the pollution and sediments deposited on the seabed. Water pH changing, can lead to the release (leaching metals in the sediment into the water body and then it will be bioaccumulated on biota arround the environment. To see the effect of pH changing on the release (leaching of these metals, extracting the sediment at pH variations has done (TCLP method. From the results of detection metals cadmium (Cd and nickel (Ni release studies, to see the hazards of cadmium and nickel metal, carried out a simulation of bioaccumulation test on biota using bioindikator Cyprinus carpio (OECD Guideline 305. Based on the analysis of data obtained in the nickel content in the sediment extract pH 3, 5 and 7 reached 2.55 to 27.94 µg/g, while for cadmium reaches 4.31 to 4.68 µg/g. Observation of metallic nickel and cadmium bioaccumulation in fish hass done for 28 days by looking at levels of cadmium and nickel on the gills of fish and meat. In the flesh of fish, the highest cadmium concentration of 3.179 µg/g while in the gills is 5.392 µg/g. The highest nickel concentrations in fish flesh is equal to 4.557 µg/g while for gill is equal to 10.417 µg/g. The study results indicate the presence of cadmium and nickel metal accumulation on biota.   Keywords: TCLP method, biota, Cyprinus carpio

  17. Efficient removal of rhodamine 6G dye from aqueous solution using nickel sulphide incorporated polyacrylamide grafted gum karaya bionanocomposite hydrogel

    CSIR Research Space (South Africa)

    Kumar, N

    2016-02-01

    Full Text Available polymer of the Gk with the PAAm was synthesized using the graft co-polymerization technique. In the second step, the nickel sulphide nanoparticles were incorporated in situ within the hydrogel polymer matrix. The synthesized hydrogel nanocomposite...

  18. Synthesis of carbon nanospheres using fallen willow leaves and adsorption of Rhodamine B and heavy metals by them.

    Science.gov (United States)

    Qu, Jiao; Zhang, Qian; Xia, Yunsheng; Cong, Qiao; Luo, Chunqiu

    2015-01-01

    This paper focuses on the synthesis of carbon nanospheres (CNSs) using fallen willow leaves as a low-cost precursor. The scanning electron microscopy (SEM) image and transmission electron microscopy (TEM) image demonstrated that the structure of synthesized CNSs was spherical, with a diameter of 100 nm. The crystal structure and chemical information were characterized by Raman spectrum and energy-dispersive spectrum (EDS), respectively. BET results showed that the CNSs had a larger specific surface area of 294.32 m(2) g(-1), which makes it a potentially superior adsorbent. Rh-B and heavy metal ions such as Cu(2+), Zn(2+), and Cr(6+) were used as targets to investigate the adsorption capacity of the CNSs. The effects of adsorption parameters such as adsorption equilibrium time, dose of CNSs, adsorption kinetics, and effect factors were also studied. These findings not only established a cost-effective method of synthesizing CNSs using fallen willow leaves but also broadened the potential application range of these CNSs.

  19. Determination of Trace Amounts of Gold in Environmental Samples by Adsorptive Stripping Voltammetry of Its Complex with Rhodamine Using Osc-Pls

    Directory of Open Access Journals (Sweden)

    A. Akrami

    2012-11-01

    Full Text Available The multivariate calibration method was applied for the determination of trace amounts of gold based on a hanging mercury drop electrode (HMDE in the presence of rhodanine, followed by reduction of adsorbed gold by voltammetric scan using differential pulse modulation The optimum experimental conditions are: rhodanine concentration of 0.20 mg mL-1, pH 5.0, accumulation potential of -600 mV versus Ag/AgCl, accumulation time of 100 sec, scan rate of 30 mV s-1 and pulse height of 100 mV. The calibration matrix for partial least squares (PLS regression was designed with 9 samples. Orthogonal signal correction (OSC is a preprocessing technique used for removing the information unrelated to the target variables based on constrained principal component analysis. OSC is a suitable preprocessing method for PLS calibration without loss of prediction capacity using electrochemical method. The RMSEP for gold determination with PLS and OSC-PLS were 8.51 and 1.94, respectively. This procedure allows the determination of gold in synthetic and real samples with good reliability of the determination. 

  20. The leaching of Rhodamine B, Naphthol Blue Black, Metanil Yellow and Bismarck Brown R from silica deposits on polyester and viscose textiles

    Czech Academy of Sciences Publication Activity Database

    Nedelčev, T.; Krupa, I.; Lath, D.; Špírková, Milena

    2008-01-01

    Roč. 46, č. 1 (2008), s. 47-56 ISSN 0928-0707 R&D Projects: GA AV ČR IAA400500505 Grant - others:Science and Technology Assistance Agency(SK) APVT-99-035004; VEGA(SK) 2/6114/26 Institutional research plan: CEZ:AV0Z40500505 Keywords : sol-gel process * polyester * viscose Subject RIV: CD - Macromolecular Chemistry Impact factor: 1.433, year: 2008

  1. Highly mesoporous α-Fe2O3 nanostructures: preparation, characterization and improved photocatalytic performance towards Rhodamine B (RhB)

    International Nuclear Information System (INIS)

    Bharathi, S; Nataraj, D; Mangalaraj, D; Masuda, Y; Senthil, K; Yong, K

    2010-01-01

    Single-crystalline porous hematite nanorods and spindle-like nanostructures were successfully synthesized by a low temperature reflux condensation method. Two different iron sources, namely, FeCl 3 ·6H 2 O and Fe(NO 3 ) 3 ·9H 2 O, were hydrolyzed in the presence of urea to selectively prepare nanorods and spindle-like nanostructures. Initially, the akagenite phase was obtained by refluxing the precursor for 12 h and then the as-prepared akagenite nanostructures were transformed to porous hematite nanostructures upon calcination at 300 0 C for 1 h. The shape and the aspect ratio of the 12 h refluxed sample was retained even after calcination and this shows the topotactic transformation of the nanostructure. TEM and HRTEM investigations have shown the porous nature of the prepared sample. Brunauer-Emmett-Teller and Barret-Joyner-Halenda measurements have shown a large surface area and distribution of mesopores in the nanorods sample. The photocatalytic activity of the prepared nanostructures towards RhB has reflected this variation in the pore size distribution and specific surface area, by showing a higher activity for the nanorods sample. Magnetic studies by VSM have shown a weak ferromagnetic behaviour in both the samples due to shape anisotropy.

  2. Highly mesoporous {alpha}-Fe{sub 2}O{sub 3} nanostructures: preparation, characterization and improved photocatalytic performance towards Rhodamine B (RhB)

    Energy Technology Data Exchange (ETDEWEB)

    Bharathi, S; Nataraj, D [Thin Films and Nanomaterials Lab, Department of Physics, Bharathiar University, Coimbatore 641046 (India); Mangalaraj, D [DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore 641046 (India); Masuda, Y [National Institute of Advanced Industrial Science and Technology (AIST), Nagoya 463-8560 (Japan); Senthil, K [Centre for Information Materials, Pohang University of Science and Technology, Pohang (Korea, Republic of); Yong, K, E-mail: de.natraj@gmail.co [Department of Chemical Engineering, Pohang University of Science and Technology, Pohang (Korea, Republic of)

    2010-01-13

    Single-crystalline porous hematite nanorods and spindle-like nanostructures were successfully synthesized by a low temperature reflux condensation method. Two different iron sources, namely, FeCl{sub 3{center_dot}}6H{sub 2}O and Fe(NO{sub 3}){sub 3{center_dot}}9H{sub 2}O, were hydrolyzed in the presence of urea to selectively prepare nanorods and spindle-like nanostructures. Initially, the akagenite phase was obtained by refluxing the precursor for 12 h and then the as-prepared akagenite nanostructures were transformed to porous hematite nanostructures upon calcination at 300 {sup 0}C for 1 h. The shape and the aspect ratio of the 12 h refluxed sample was retained even after calcination and this shows the topotactic transformation of the nanostructure. TEM and HRTEM investigations have shown the porous nature of the prepared sample. Brunauer-Emmett-Teller and Barret-Joyner-Halenda measurements have shown a large surface area and distribution of mesopores in the nanorods sample. The photocatalytic activity of the prepared nanostructures towards RhB has reflected this variation in the pore size distribution and specific surface area, by showing a higher activity for the nanorods sample. Magnetic studies by VSM have shown a weak ferromagnetic behaviour in both the samples due to shape anisotropy.

  3. In situ grown hierarchical 50%BiOCl/BiOI hollow flowerlike microspheres on reduced graphene oxide nanosheets for enhanced visible-light photocatalytic degradation of rhodamine B

    Science.gov (United States)

    Su, Xiangde; Yang, Jinjin; Yu, Xiang; Zhu, Yi; Zhang, Yuanming

    2018-03-01

    50%BiOCl/BiOI/reduced graphene oxide (50%BiOCl/BiOI/rGO) composite photocatalyst was synthesized successfully by a facile one-step solvothermal route in this work. Reduction of graphene oxide (GO) took place in the process of solvothermal reaction and a new Bi-C bond between rGO and 50%BiOCl/BiOI was formed. The introduction of rGO affected the morphology of 50%BiOCl/BiOI, resulting in the transformation of 50%BiOCl/BiOI from solid microspheres to hollow microspheres. Both the introduction of rGO and formation of 50%BiOCl/BiOI hollow microspheres can facilitate the light absorption. The strong interaction between 50%BiOCl/BiOI and rGO and the electrical conductivity of rGO greatly improved the effective separation of photogenerated carriers. Hence, GOB-5 demonstrated the highest photocatalytic activity which was over twice of the pristine 50%BiOCl/BiOI in the presence of visible light. Mechanism study revealed that 50%BiOCl/BiOI generated electrons and holes in the presence of visible light, and holes together with rad O2- generated from reduction of O2 by electrons degraded the pollutant directly. Overall, this work provides an excellent reference to the synthesis of chemically bonded BiOX/BiOY (X, Y = Cl, Br, I)/rGO nanocomposite and helps to promote their applications in environmental protection and photoelectric conversion.

  4. Chemically stable and reusable nano zero-valent iron/graphite-like carbon nitride nanohybrid for efficient photocatalytic treatment of Cr(VI) and rhodamine B under visible light

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Zhiyu; Wen, Qingjuan; Wang, Xiu; Zhang, Fuwei [Key Laboratory of Eco-materials Advanced Technology (Fuzhou University), Fujian Province University (China); College of Materials Science and Engineering, Fuzhou University, New Campus, Minhou, Fujian Province 350108 (China); Yu, Yan, E-mail: yuyan@fzu.edu.cn [Key Laboratory of Eco-materials Advanced Technology (Fuzhou University), Fujian Province University (China); College of Materials Science and Engineering, Fuzhou University, New Campus, Minhou, Fujian Province 350108 (China)

    2016-11-15

    Highlights: • NZVI-g-C{sub 3}N{sub 4} efficiently photodegrades the organic/inorganic pollutants. • Fe{sup 0} improves the charge separation efficiency of g-C{sub 3}N{sub 4}. • A possible recover mechanism of nZVI-g-C{sub 3}N{sub 4} was proposed. - Abstract: Graphite-like carbon nitride (g-C{sub 3}N{sub 4}) displays strong potential applications in visible-light photocatalytic for water treatment, but its applications are greatly restricted by high recombination probability of photo-generated electron-hole pairs, as well as a weak reduction ability toward the heavy metals. In this work, we reported the synthesis of nZVI-g-C{sub 3}N{sub 4} nano-hybrid with highly efficiency toward the photodegradation of RhB and Cr(VI) under the visible light irradiation. The nZVI nanoparticles can well be immobilized and dispersed on the surface of g-C{sub 3}N{sub 4} nanosheets by a facile borohydride-reduction method. As-synthesized nZVI-g-C{sub 3}N{sub 4} has an improved photocatalytic activity much better than that of the pure g-C{sub 3}N{sub 4}, wherein over 92.9% of Cr(VI) and 99.9% of RhB can be removed by using nZVI-g-C{sub 3}N{sub 4}. The nZVI particles not only contributes to the reduction and immobilization of Cr(VI), but also accelerates the photocatalytic degradation efficiency of RhB due to a lower recombination rate of photoexcited holes and electrons. Moreover, nZVI-g-C{sub 3}N{sub 4} preserves superior photodegradation efficiency after five experimental cycles. It can be attributed that nZVI-g-C{sub 3}N{sub 4} photocatalyst is chemically stable, and part of nZVI can be recovered by g-C{sub 3}N{sub 4}. We believe that, the composite of nZVI-g-C{sub 3}N{sub 4} reported here could provide guidance for the design of efficient and reusable materials to remove both the organic compounds and heavy metal ions from waste waters.

  5. Study of internalization and viability of multimodal nanoparticles for labeling of human umbilical cord mesenchymal stem cells; Estudo de internalizacao e viabilidade de nanoparticulas multimodal para marcacao de celulas-tronco mesenquimais de cordao umbilical humano

    Energy Technology Data Exchange (ETDEWEB)

    Miyaki, Liza Aya Mabuchi [Faculdade de Enfermagem, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Sibov, Tatiana Tais; Pavon, Lorena Favaro; Mamani, Javier Bustamante; Gamarra, Lionel Fernel, E-mail: tatianats@einstein.br [Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil)

    2012-04-15

    Objective: To analyze multimodal magnetic nanoparticles-Rhodamine B in culture media for cell labeling, and to establish a study of multimodal magnetic nanoparticles-Rhodamine B detection at labeled cells evaluating they viability at concentrations of 10 {mu}g Fe/mL and 100{mu}g Fe/mL. Methods: We performed the analysis of stability of multimodal magnetic nanoparticles-Rhodamine B in different culture media; the mesenchymal stem cells labeling with multimodal magnetic nanoparticles-Rhodamine B; the intracellular detection of multimodal magnetic nanoparticles-Rhodamine B in mesenchymal stem cells, and assessment of the viability of labeled cells by kinetic proliferation. Results: The stability analysis showed that multimodal magnetic nanoparticles-Rhodamine B had good stability in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium. The mesenchymal stem cell with multimodal magnetic nanoparticles-Rhodamine B described location of intracellular nanoparticles, which were shown as blue granules co-localized in fluorescent clusters, thus characterizing magnetic and fluorescent properties of multimodal magnetic nanoparticles Rhodamine B. Conclusion: The stability of multimodal magnetic nanoparticles-Rhodamine B found in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium assured intracellular mesenchymal stem cells labeling. This cell labeling did not affect viability of labeled mesenchymal stem cells since they continued to proliferate for five days. (author)

  6. Study of internalization and viability of multimodal nanoparticles for labeling of human umbilical cord mesenchymal stem cells

    International Nuclear Information System (INIS)

    Miyaki, Liza Aya Mabuchi; Sibov, Tatiana Tais; Pavon, Lorena Favaro; Mamani, Javier Bustamante; Gamarra, Lionel Fernel

    2012-01-01

    Objective: To analyze multimodal magnetic nanoparticles-Rhodamine B in culture media for cell labeling, and to establish a study of multimodal magnetic nanoparticles-Rhodamine B detection at labeled cells evaluating they viability at concentrations of 10 μg Fe/mL and 100μg Fe/mL. Methods: We performed the analysis of stability of multimodal magnetic nanoparticles-Rhodamine B in different culture media; the mesenchymal stem cells labeling with multimodal magnetic nanoparticles-Rhodamine B; the intracellular detection of multimodal magnetic nanoparticles-Rhodamine B in mesenchymal stem cells, and assessment of the viability of labeled cells by kinetic proliferation. Results: The stability analysis showed that multimodal magnetic nanoparticles-Rhodamine B had good stability in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium. The mesenchymal stem cell with multimodal magnetic nanoparticles-Rhodamine B described location of intracellular nanoparticles, which were shown as blue granules co-localized in fluorescent clusters, thus characterizing magnetic and fluorescent properties of multimodal magnetic nanoparticles Rhodamine B. Conclusion: The stability of multimodal magnetic nanoparticles-Rhodamine B found in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium assured intracellular mesenchymal stem cells labeling. This cell labeling did not affect viability of labeled mesenchymal stem cells since they continued to proliferate for five days. (author)

  7. Photocatalytic Degradation Property of NANO-TiO2/DIATOMITE for Rodamine B Dye Wastewater

    Science.gov (United States)

    Liu, Yue; Zheng, Shuilin; Du, Gaoxiang; Shu, Feng; Chen, Juntao

    The Nano-TiO2/Diatomite compound photocatalyst is used to degrade rhodamine B dye wastewater in photochemical reactor. The test result indicates that the rate of photodegradation of rhodamine B is influenced by reactive conditions. The best technical conditions are concentration of rhodamine B solution 10mg/L, ultraviolet light 300W, the compound photocatalyst amount used 1g/L, the pH 5.8, reaction time 20min. Under these conditions the rate of photodegradation of rhodamine B may reach as high as 97.80%. And the efficiency of photodegradation of catalyst only has a little changed in recycling.

  8. Synthesis, characterization and ion recognition studies of lower rim ...

    Indian Academy of Sciences (India)

    Keywords. Rhodamine conjugate of calix[4]arene; ratiometric sensor for Hg2+; ion recognition; emission ... rhodamine-based receptor is dependent on the changes that occur in ..... (DRDL) for allowing him to register for Ph.D. pro- gramme at ...

  9. Micro-fabricated solid state dye lasers based on a photo-definable polymer

    DEFF Research Database (Denmark)

    Nilsson, Daniel; Balslev, Søren; Gregersen, Misha Marie

    2005-01-01

    the commercially available laser dye Rhodamine 6G, which is incorporated into the SU-8 polymer matrix. The single-mode slab waveguide is formed by three-step spin-coating deposition: a buffer layer of undoped SU-8, a core layer of SU-8 doped with Rhodamine, and a cladding layer of undoped SU-8. (c) 2005 Optical...

  10. for aqueous dye lasers

    Indian Academy of Sciences (India)

    2014-02-12

    Feb 12, 2014 ... inclusion complex of RhB with the container molecule cucurbit[7]uril (CB[7]). Keywords. Temperature-dependent fluorescence; Rhodamine B; cucurbit[7]uril; host–guest complex; dye laser. PACS Nos 36.20.kd; 83.60.pq; 87.64.kv. 1. Introduction. Rhodamine B (RhB) is an efficient and photostable laser dye ...

  11. Kandungan bahan tambahan pangan berbahaya pada makanan jajanan anak sekolah dasar di Kabupaten Bantul

    Directory of Open Access Journals (Sweden)

    Yhona Paratmanitya

    2016-08-01

    Full Text Available ABSTRACTBackground: The National Agency of Drug and Food Control (BPOM showed that in 2012, consuming of food is the highest caused of poisoning incidence (66.7% among others (drug, cosmetics, etc. One of factors influence it was chemical contamination in food, such as borax (sodium tetraborate, formaldehyde, and rhodamine-B. Based on this data, there were 2.93% of borax, 1.34% of formaldehyde, and 1.02% of rhodamine-B detected in the snack foods of elementary school children. Their accumulation may have negative impact on body health.Objectives: To know the content of food additive substances contamination (borax, formaldehyde, rhodamin-B in the snack food of elementary school children in Bantul. Methods: This was an observational research with survey design. The research was done in 68 of elementary schools in District of Bantul. All suspected snack food samples inside and outside the school were analyzed for their chemical contaminations. Qualitative analysis of borax, formaldehyde, and rhodamin-B were done by using curcumin, KMnO4, and test kit methods, respectively. Results: There were 107 samples collected from 68 elementary schools in Bantul. The most sold sample suspecting of chemical contamination was meatball (22.4%. Among 98 analyzed samples, there were 15 (15.3% and 25 (25.5% samples that were proven to have borax and formaldehyde. Meanwhile, there were 7 (46.7% samples of 15 samples were proven to have rhodamine-B. From the data, there were 34 elementary schools (50% that have no harmful chemical contamination in their snack food.Conclusions: The percentage of snack food containing borax, formaldehyde, and rhodamine-B in elementary school in Bantul was still high enough.KEYWORDS: snack food, borax, formaldehyde, rhodamine-BABSTRAKLatar belakang: Data Badan Pengawas Obat dan Makanan (BPOM menunjukkan bahwa sepanjang tahun 2012, insiden keracunan akibat mengkonsumsi makanan menduduki posisi paling tinggi, yaitu 66,7%, dibandingkan

  12. Efficiencies of laser dyes for atomic vapor laser isotope separation

    International Nuclear Information System (INIS)

    Maeda, Mitsuo; Oki, Yuji; Uchiumi, Michihiro; Takao, Takayuki; Igarashi, Kaoru; Shimamoto, Kojiro.

    1995-01-01

    Efficiencies of 30 laser dyes for the atomic vapor laser isotope separation (AVLIS) are experimentally evaluated with a dye laser pumped by a frequency-doubled Nd:YAG laser. On the other hand, a simulation code is developed to describe the laser action of Rhodamine 6G, and the dependence of the laser efficiency on the pump wavelength is calculated. Following conclusions are obtained by these considerations:space: 1) Pyrromethene 567 showed 16% higher laser efficiency than Rhodamine 6G by 532 nm pumping, and Pyrromethene 556 has an ability to provide better efficiency by green light pumping with a Cu vapor laser; 2) Kiton red 620 and Rhodamine 640, whose efficiencies were almost the same as Rhodamine 6G by 532 nm pumping, will show better efficiencies by two-wavelength pumping with a Cu vapor laser. (author)

  13. Efficient photocatalytic activity with carbon-doped SiO2 nanoparticles

    KAUST Repository

    Zhang, Dongen; Wu, Jinbo; Zhou, Bingpu; Hong, Yaying; Li, Shunbo; Wen, Weijia

    2013-01-01

    by thermogravimetric analysis, X-ray diffraction, standard and high resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The C-doped SiO2 displayed outstanding photocatalytic properties, as evidenced by its catalysis of Rhodamine B

  14. Acquired mutations in the MXR/BCRP/ABCP gene alter substrate specificity in MXR/BCRP/ABCP-overexpressing cells

    DEFF Research Database (Denmark)

    Honjo, Y; Hrycyna, C A; Yan, Q W

    2001-01-01

    A disparity was noted in the transport of rhodamine 123 among nine MXR/BCRP/ABCP-overexpressing cells studied; all demonstrated mitoxantrone transport, whereas only two effluxed rhodamine 123. When the MXR/BCRP/ABCP gene was sequenced in the cell lines studied, differences were noted at amino acid...... 482, predicted to be at the start of the third transmembrane domain. Sequencing genomic DNA revealed wild-type MXR/BCRP/ABCP to have an arginine at position 482. Cells having a threonine or glycine at position 482 were able to efflux rhodamine 123, whereas cells having an arginine were not. A vaccinia...... virus expression system confirmed that rhodamine as well as doxorubicin efflux is observed with R482T or R482G but not with the wild-type R482; all three MXR/BCRP/ABCP forms transported mitoxantrone. Cross-resistance studies suggest that, compared with wild-type MXR/BCRP/ABCP, cells having an R482T...

  15. IR, 1H-NMR, UV-Vis AND THERMAL STUDIES ON THE ...

    African Journals Online (AJOL)

    Preferred Customer

    1Department of Chemistry, Faculty of Science, Port Said University, Port Said ... The general chemical structure of Rhodamine 6G donor and acceptors are given in .... atom of the -NH group is involved in the complexation process between ...

  16. Photocatalytic hydrogen generation with Ag-loaded LiNbO3

    Indian Academy of Sciences (India)

    Administrator

    rhodamine B decomposition under solar illumination was also reported (Stock ... photocatalytic hydrogen production. ... lytic hydrogen production from a methanol aqueous solu- .... estimated bandgap energy of Nb2O5 and LiNbO3 samples.

  17. Two-dimensional electronic femtosecond stimulated Raman spectroscopy

    Directory of Open Access Journals (Sweden)

    Ogilvie J.P.

    2013-03-01

    Full Text Available We report two-dimensional electronic spectroscopy with a femtosecond stimulated Raman scattering probe. The method reveals correlations between excitation energy and excited state vibrational structure following photoexcitation. We demonstrate the method in rhodamine 6G.

  18. Photophysical properties of some xanthylium salts performances under CVL pumping

    International Nuclear Information System (INIS)

    Doizi, D.; Lompre, L.A.; Gazeau, M.C.

    1995-01-01

    We report the photochemical and photophysical performances of some new dyes belonging to the xanthylium salts family. Performances under Copper Vapor Laser (CVL) pumping are described and compared to those of Rhodamine 6G. (author)

  19. Gold Nanoparticle-Based Detection of Hg(II) in an Aqueous Solution: Fluorescence Quenching and Surface-Enhanced Raman Scattering Study

    International Nuclear Information System (INIS)

    Ganbold, Erdene Ochir; Park, Jin Ho; Ock, Kwang Su; Joo, Sang Woo

    2011-01-01

    We studied the detection of the Hg(II) concentration in an aqueous solution using rhodamine dyes on citrate-reduced Au nanoparticles (NPs). The quenching effect from Au NPs was found to decrease as the Hg(II) concentration increased under our experimental conditions. As the fluorescence signals intensified, the surface-enhanced Raman scattering (SERS) intensities reduced on the contrary due to less rhodamine dyes on Au NPs as the Hg(II) concentration increased. The rhodamine 6G (Rh6G) and rhodamine 123 (Rh123) dyes were examined via fluorescence and SERS measurements depending on Hg(II) concentrations. Fast and easy fluorescence detection of an Hg (II) concentration as low as a few ppm could be achieved by naked eye using citrate-reduced Au NPs

  20. Synthesis of fluorescent diblock copolymer nanoparticle supported ...

    Indian Academy of Sciences (India)

    2017-06-09

    Jun 9, 2017 ... and induction time (Ti) were determined from the UV–visible spectral data. ... The photo- degradation of rhodamine dye was carried out in the presence ... ence of hierarchical structured polystyrene/polyaniline@Au.

  1. Bibliographic study of photophysical and photochemical properties of laser dyes

    International Nuclear Information System (INIS)

    Doizi, D.

    1986-06-01

    Laser isotope separation of uranium requires high power and precise wave length. This report is a bibliographic and experimental study of the photophysical and photochemical properties of seven commercial laser dyes which have an emission wavelength in the range 5500-6500 A: Rhodamine 110 or 560, rhodamine 6G or 590, rhodamine B or 610, rhodamine 101 or 640, sulforhodamine B or kiton red 620, sulforhodamine 101 or 640 and DCM or LC 6500. Absorption and emission cross section values, fluorescence lifetimes and quantum yields in various solvents are indicated. For each dye, a non exhaustive list of laboratory experiments made with two types of pump sources: Nd YAG (532) and copper vapor laser is given. When it is known, the toxicity of the dyes is mentioned [fr

  2. Synthesis and characterization of fluorophore attached silver ...

    Indian Academy of Sciences (India)

    Administrator

    in protein detection (Nam et al 2003), labeling agents. (Tkachenko et al ... these chemicals are highly reactive and pose potential environmental and ... The interaction of rhodamine 6G with ... lar excretion of the kidney although the electrostatic.

  3. [A novel yellow organic light-emitting device].

    Science.gov (United States)

    Ma, Chen; Wang, Hua; Hao, Yu-Ying; Gao, Zhi-Xiang; Zhou, He-Feng; Xu, Bing-She

    2008-07-01

    The fabrication of a novel organic yellow-light-emitting device using Rhodamine B as dopant with double quantum-well (DQW) structure was introduced in the present article. The structure and thickness of this device is ITO/CuPc (6 nm) /NPB (20 nm) /Alq3 (3 nm)/Alq3 : Rhodamine B (3 nm) /Alq3 (3 nm) /Al q3 : Rhodamine B(3 nm) /Alq3 (30 nm) /Liq (5 nm)/Al (30 nm). With the detailed investigation of electroluminescence of the novel organic yellow-light-emitting device, the authors found that the doping concentration of Rhodamine B (RhB) had a very big influence on luminance and efficiency of the organic yellow-light-emitting device. When doping concentration of Rhodamine B (RhB) was 1.5 wt%, the organic yellow-light-emitting device was obtained with the maximum current efficiency of 1.526 cd x A(-1) and the maximum luminance of 1 309 cd x m(-2). It can be seen from the EL spectra of the devices that there existed energy transferring from Alq3 to RhB in the organic light-emitting layers. When the doping concentration of RhB increased, lambda(max) of EL spectra redshifted obviously. The phenomenon was attributed to the Stokes effect of quantum wells and self-polarization of RhB dye molecules.

  4. Ratiometric fluorescent nanosensor based on carbon dots for the detection of mercury ion

    Science.gov (United States)

    Ma, Yusha; Mei, Jing; Bai, Jianliang; Chen, Xu; Ren, Lili

    2018-05-01

    A novel ratiometric fluorescent nanosensor based on carbon dots has been synthesized via bonding rhodamine B hydrazide to the carbon dots surface by an amide reaction. The ratiometric fluorescent nanosensor showed only a single blue fluorescence emission around 450 nm. While, as mercury ion was added, due to the open-ring of rhodamine moiety bonded on the CDs surface, the orange emission of the open-ring rhodamine would increase obviously according to the concentration of mercury ion, resulting in the distinguishable dual emissions at 450 nm and 575 nm under a single 360 excitation wavelength. Meanwhile, the ratiometric fluorescent nanosensor based on carbon dots we prepared is more sensitive to qualitative and semi-quantitative detection of mercury ion in the range of 0–100 μM, because fluorescence changes gradually from blue to orange emission under 365 nm lamp with the increasing of mercury ion in the tested solution.

  5. Synthesis and characterisation of new laser dyes

    International Nuclear Information System (INIS)

    Scala-Valero, Claudine

    1997-01-01

    Rhodamines are very efficient laser dyes for the red part of the visible spectrum: their fluorescence quantum efficiencies are about hundred per cent. However, their conversion efficiencies in dye amplifier are about fifty per cent, due to the presence of S n electronic level which is responsible of the re-absorption of a part of photons. In this research thesis, the author aims at trying to move this S n level out of the stimulated emission bandwidth. Models have been developed to propose new structures derived from rhodamines and theoretically possessing the desired properties. The so-recommended molecules have then been synthesised and characterised in terms of absorption and fluorescence spectra, of quantum efficiency, and of fluorescence lifetime. Two modifications are proposed for the rhodamine 6G structure, either by grafting methyl substitutes, or by grafting variously substituted amines. The searched result is obtained with the second modification [fr

  6. Investigation of the lasing of dyes under copper vapor laser irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Danilova, V I; Kopylova, T N; Maier, G V; Masarnovskii, L V; Soldatov, A N; Sukhanov, V B

    1980-10-01

    The lasing characteristics of dyes pumped by copper vapor laser radiation are investigated in order to determine the optimal energetic parameters of the dye-laser system. Expressions are derived for the yields of stimulated emission from dye molecules, and it is shown that the most effective means of improving the lasing characteristics of rhodamine dye solutions is by the modification of intermolecular interactions, in part by the use of multicomponent solutions. Results are then presented of experimental measurements of the emission intensities of combinations of rhodamine dyes irradiated by the 5106-A line of a copper vapor laser. An increase in the lasing efficiency of the acceptor molecule is found for all the dye pairs investigated, with even greater emission intensities observed for multicomponent dye mixtures when the mixtures were pumped transversely. Under longitudinal pumping, improvements in lasing efficiency were obtained only for mixtures of rhodamine 6 Zh with cresil violet.

  7. Mitochondrial activity assessed by cytofluorescence after in-vitro-irradiation of primary rat brain cultures

    International Nuclear Information System (INIS)

    Cervos-Navarro, J.; Hamdorf, G.

    1993-01-01

    Mitochondria play a key role in cell homeostasis and are the first cell organells affected by ionizing irradiation, as it was proved by previous electron microscopic investigations. In order to observe functional parameters of mitochondria after low-dose irradiation, primary rat brain cultures (prepared from 15-day-old rat fetuses) were irradiated from a 60 Co-source with 0.5 and 1 Gy at the age of 2 or 7 days in vitro (div). Cytofluorescence measurement was made by a Cytofluor trademark2350 using Rhodamine 123. This fluorescent dye is positively charged and accumulates specifically in the mitochondria of living cells without cytotoxic effect. Since its retention depends on the negative membrane potential as well as the proton gradient that exists across the inner mitochondrial membrane, Rhodamine 123 accumulation reflects the status of mitochondrial activity as a whole. After irradiation with 0.5 and 1 Gy on day 2 in culture there was a decrease in Rhodamine uptake in the irradiated cultures during the first week after the irradiation insult which reached minimum values after 3 days. Rhodamine uptake increased during the following period and finally reached the values of the control cultures. In the second experiment with irradiated cultures on day 7 and the same doses of 0.5 and 1 Gy the accumulation of Rhodamine decreased only initially then increased tremendously. After both doses values of Rhodamine-accumulation were higher than the control level. The results demonstrated that irradiation caused a change in mitochondrial activity depending on the time of irradiation. The dramatic increase over the control levels after irradiation on day 7 in vitro is attributed to the fact that at this time synapses have already developed. Deficiency of mitochondrial activity as well as hyperactivity and the consequent change in energy production may lead to changes in neuronal metabolism including an increase in production of free radicals

  8. Fluorescence detection of single molecules using pulsed near-field optical excitation and time correlated photon counting

    International Nuclear Information System (INIS)

    Ambrose, W.P.; Goodwin, P.M.; Martin, J.C.; Keller, R.A.

    1994-01-01

    Pulsed excitation, time correlated single photon counting and time gated detection are used in near-field optical microscopy to enhance fluorescence images and measure the fluorescence lifetimes of single molecules of Rhodamine 6G on silica surfaces. Time gated detection is used to reject prompt scattered background and to improve the image signal to noise ratio. The excited state lifetime of a single Rhodamine 6G molecule is found to depend on the position of the near-field probe. We attribute the lifetime variations to spontaneous emission rate alterations by the fluorescence reflected from and quenching by the aluminum coated probe

  9. Optical study of planar waveguides based on oxidized porous silicon impregnated with laser dyes

    Energy Technology Data Exchange (ETDEWEB)

    Chouket, A. [Unite de recherche de Spectroscopie Raman, Departement de Physique, Faculte des Sciences de Tunis, Elmanar 2092, Tunis (Tunisia); Charrier, J. [Laboratoire d' Optronique CNRS-UMR FOTON 6082, Universite de Rennes 1, ENSSAT-6 rue de Kerampont, BP 80518, 22305 Lannion Cedex (France); Elhouichet, H. [Unite de recherche de Spectroscopie Raman, Departement de Physique, Faculte des Sciences de Tunis, Elmanar 2092, Tunis (Tunisia)], E-mail: habib.elhouichet@fst.rnu.tn; Oueslati, M. [Unite de recherche de Spectroscopie Raman, Departement de Physique, Faculte des Sciences de Tunis, Elmanar 2092, Tunis (Tunisia)

    2009-05-15

    Oxidized porous silicon optical planar waveguides were elaborated and impregnated with rhodamine B and rhodamine 6G. The waveguiding, absorption, and photoluminescence properties of these impregnated waveguides were studied. Successful impregnation of the structure with laser dyes is shown from photoluminescence and reflectivity measurements. Furthermore, the reflectivity spectra prove the homogenous incorporation of both dye molecules inside the pores of the matrices. The refractive indices of waveguide layers were determined before and after dye impregnation to indicate the conservation of guiding conditions. The optical losses in the visible wavelengths are studied as a function of dye concentration. The dye absorption is the main reason for these losses.

  10. Surface-enhanced Raman spectroscopy on novel black silicon-based nanostructured surfaces

    DEFF Research Database (Denmark)

    Talian, Ivan; Mogensen, Klaus Bo; Orinak, A.

    2009-01-01

    , effects of Ti and Ti/Pt adhesion layers underneath the gold layers on the analytical signal enhancement were tested. An enhancement factor of 7.6 x 10(7) with the excitation laser 785 nm was achieved for the tested analyte, Rhodamine 6G, and non-resonance SER spectra were recorded in a 5 s acquisition...... mode. Such an enhancement enables to achieve a detection limit down to 2.4 pg of Rhodamine 6G on a black silicon-based nanosurface coated with a 400-nm-thin layer of gold....

  11. Investigation of the dye concentration influence on the lasing wavelength and threshold for a micro-fluidic dye laser

    DEFF Research Database (Denmark)

    Helbo, Bjarne; Kragh, Søren; Kjeldsen, B.G.

    2003-01-01

    We investigate a micro-fluidic dye laser, which can be integrated with polymer-based lab-on-a-chip microsystems without further processing steps. A simple rate-equation model is used to predict the lasing threshold. The laser device is characterised using the laser dye Rhodamine 6G dissolved...... in ethanol, and the influence of dye concentration on the lasing wavelength and threshold is investigated. The experiments confirm the predictions of the rate-equation model, that lasing can be achieved in the 10 mum long laser cavity with moderate concentrations of Rhodamine 6G in ethanol, starting from 5 x...

  12. Highly selective and reversible chemosensor for Pd(2+) detected by fluorescence, colorimetry, and test paper.

    Science.gov (United States)

    Wang, Mian; Liu, Xiaomei; Lu, Huizhe; Wang, Hongmei; Qin, Zhaohai

    2015-01-21

    A "turn-on" fluorescent and colorimetric chemosensor (RBS) for Pd(2+) has been designed and synthesized through introduction of sulfur as a ligand atom to Rhodamine B. RBS exhibits high selectivity (freedom from the interference of Hg(2+ )in particular) and sensitivity toward Pd(2+) with a detection limit as low as 2.4 nM. RBS is also a reversible sensor, and it can be made into test paper to detect Pd(2+) in pure water. Compared to the chemosensors that introduced phosphorus to Rhodamine to detect Pd(2+), RBS can be synthesized more simply and economically.

  13. Laser generation in opal-like single-crystal and heterostructure photonic crystals

    Science.gov (United States)

    Kuchyanov, A. S.; Plekhanov, A. I.

    2016-11-01

    This study describes the laser generation of a 6Zh rhodamine in artificial opals representing single-crystal and heterostructure films. The spectral and angular properties of emission and the threshold characteristics of generation are investigated. In the case where the 6Zh rhodamine was in a bulk opal, the so-called random laser generation was observed. In contrast to this, the laser generation caused by a distributed feedback inside the structure of the photonic bandgap was observed in photonic-crystal opal films.

  14. Magnetothermal release of payload from iron oxide/silica drug delivery agents

    Energy Technology Data Exchange (ETDEWEB)

    Luong, T.T., E-mail: thientai.luong@chem.kuleuven.be [KU Leuven, Department of Chemistry, Celestijnenlaan 200D, Heverlee 3001 (Belgium); Hanoi National University of Education, Faculty of Chemistry, Xuan Thuy 136, Cau Giay, Hanoi (Viet Nam); Knoppe, S.; Bloemen, M.; Brullot, W.; Strobbe, R. [KU Leuven, Department of Chemistry, Celestijnenlaan 200D, Heverlee 3001 (Belgium); Locquet, J.-P. [KU Leuven, Department of Physics, Celestijnenlaan 200D, Heverlee 3001 (Belgium); Verbiest, T. [KU Leuven, Department of Chemistry, Celestijnenlaan 200D, Heverlee 3001 (Belgium)

    2016-10-15

    The release of covalently bound Rhodamine B from iron oxide/mesoporous silica core/shell nanoparticles under magnetically induced heating was studied. The system acts as a model to study drug delivery and payload release under magnetothermal heating. - Graphical abstract: The release of covalently bound Rhodamine B from iron oxide/mesoporous silica core/shell nanoparticles under magnetically induced heating was studied. - Highlights: • Iron oxide/mesoporous-SiO{sub 2} core-shell NPs were synthesized. • The dye was covalently bound to SiO{sub 2} shells. • The release of dye under magnetothermal heating was studied. • The results are relevant for controlled drug release.

  15. LASERS, ACTIVE MEDIA: The aqueous-polyelectrolyte dye solution as an active laser medium

    Science.gov (United States)

    Akimov, A. I.; Saletskii, A. M.

    2000-11-01

    The spectral, luminescent, and lasing properties of aqueous solutions of a cationic dye rhodamine 6G with additions of anion polyelectrolytes — polyacrylic and polymethacrylic acids — are studied. It is found that the energy and spectral properties of lasing of these solutions depend on the ratio of concentrations of polyelectrolyte and molecules. It is also found that the lasing parameters of aqueous-polyelectrolyte dye solutions can be controlled by changing the structure of the molecular system. The variation in the structure of aqueous-polyelectrolyte dye solutions of rhodamine 6G resulted in an almost five-fold increase in the lasing efficiency compared to that in aqueous dye solutions.

  16. Real-time tunability of chip-based light source enabled by microfluidic mixing

    DEFF Research Database (Denmark)

    Olsen, Brian Bilenberg; Rasmussen, Torben; Balslev, Søren

    2006-01-01

    We demonstrate real-time tunability of a chip-based liquid light source enabled by microfluidic mixing. The mixer and light source are fabricated in SU-8 which is suitable for integration in SU-8-based laboratory-on-a-chip microsystems. The tunability of the light source is achieved by changing...... the concentration of rhodamine 6G dye inside two integrated vertical resonators, since both the refractive index and the gain profile are influenced by the dye concentration. The effect on the refractive index and the gain profile of rhodamine 6G in ethanol is investigated and the continuous tuning of the laser...

  17. Single-mode solid-state polymer dye laser fabricated with standard I-line UV lithography

    DEFF Research Database (Denmark)

    Balslev, Søren; Mironov, Andrej; Nilsson, Daniel

    2005-01-01

    We present single-mode solid-state polymer dye lasers fabricated with standard UV lithography. The lasers use a high-order Bragg grating and rely on index-tuning of a photosensitive polymer for waveguiding. The gain medium is Rhodamine 6G.......We present single-mode solid-state polymer dye lasers fabricated with standard UV lithography. The lasers use a high-order Bragg grating and rely on index-tuning of a photosensitive polymer for waveguiding. The gain medium is Rhodamine 6G....

  18. Zhu Qing Wang

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences. Zhu Qing Wang. Articles written in Journal of Chemical Sciences. Volume 121 Issue 4 July 2009 pp 543-548. Highly sensitive optical chemosensor for the detection of Cu using a rhodamine B spirolatam · Gen Hua Wu Dong Xiang Wang Da Yu Wu Yuan Gao Zhu Qing Wang.

  19. Synthesis and characterization of photoswitchable fluorescent silica nanoparticles

    NARCIS (Netherlands)

    Fölling, J.; Polyakova, S.; Belov, V.; van Blaaderen, A.; Bossi, M.L.; Hell, S.W.

    2008-01-01

    We have designed and synthesized a new functional (amino reactive) highly efficient fluorescent molecular switch (FMS) with a photochromic diarylethene and a rhodamine fluorescent dye. The reactive group in this FMS -N-hydroxysuccinimide ester- allows selective labeling of amino containing molecules

  20. Reversal of resistance by GF120918 in cell lines expressing the ABC half-transporter, MXR

    DEFF Research Database (Denmark)

    de Bruin, M; Miyake, K; Litman, Thomas

    1999-01-01

    -80, a subline expressing a newly identified mitoxantrone transporter, MXR. GF120918 was ineffective in sensitizing MRP-overexpressing MCF-7 VP-16 cells to etoposide as determined by cytotoxicity studies. In flow cytometry experiments, rhodamine 123 efflux in S1-B1-20 cells was decreased at GF120918...

  1. Mathematical Modeling and Experimental Validation of Nanoemulsion-Based Drug Transport across Cellular Barriers.

    Science.gov (United States)

    Kadakia, Ekta; Shah, Lipa; Amiji, Mansoor M

    2017-07-01

    Nanoemulsions have shown potential in delivering drug across epithelial and endothelial cell barriers, which express efflux transporters. However, their transport mechanisms are not entirely understood. Our goal was to investigate the cellular permeability of nanoemulsion-encapsulated drugs and apply mathematical modeling to elucidate transport mechanisms and sensitive nanoemulsion attributes. Transport studies were performed in Caco-2 cells, using fish oil nanoemulsions and a model substrate, rhodamine-123. Permeability data was modeled using a semi-mechanistic approach, capturing the following cellular processes: endocytotic uptake of the nanoemulsion, release of rhodamine-123 from the nanoemulsion, efflux and passive permeability of rhodamine-123 in aqueous solution. Nanoemulsions not only improved the permeability of rhodamine-123, but were also less sensitive to efflux transporters. The model captured bidirectional permeability results and identified sensitive processes, such as the release of the nanoemulsion-encapsulated drug and cellular uptake of the nanoemulsion. Mathematical description of cellular processes, improved our understanding of transport mechanisms, such as nanoemulsions don't inhibit efflux to improve drug permeability. Instead, their endocytotic uptake, results in higher intracellular drug concentrations, thereby increasing the concentration gradient and transcellular permeability across biological barriers. Modeling results indicated optimizing nanoemulsion attributes like the droplet size and intracellular drug release rate, may further improve drug permeability.

  2. Bulletin of Materials Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    By employing a 'single-touch contact' method, we reveal the ability of these probes to detect routinely used Raman markers such as 2-napthalenethiol and rhodamine B, at nano-molar concentrations, in dry and liquid forms, respectively. Reproducibility of the signals with variation <5%, and the ability to detect biomolecules ...

  3. Monitoring rodents movements with a biomarker around introduction and feeding foci in an urban environment in Tanzania

    DEFF Research Database (Denmark)

    Mohr, Katrine; Leirs, Herwig; Katakweba, Abdul

    2007-01-01

    , Rhodamine B (RB), we investigated the distances over which rodents are active around such potential introduction sites. Animals feeding at such sites were traced up to 100 m distant within a period of 10 days.We found that RB is a practical alternative for studying rodent movements. These results may be...

  4. Oxygen dynamics and porewater transport in sediments inhabited by the invasive polychaete Marenzelleria viridis

    DEFF Research Database (Denmark)

    Jovanovic, Zeljko; Larsen, Morten; Quintana, Cintia Organo

    2014-01-01

    (rhodamine WT and brilliant blue), we investigated the relationship between irrigation and O2 dynamics in burrows of M. viridis. The investigated animals shifted between 2 modes of ventilation: ciliary pumping for 77% of the time and muscular pumping for 23% of the time. On average, muscular pumping...

  5. Surface-Enhanced Raman Scattering Activity of Ag/graphene/polymer Nanocomposite Films Synthesized by Laser Ablation

    Czech Academy of Sciences Publication Activity Database

    Siljanovska Petreska, G.; Blazevska-Gilev, J.; Fajgar, Radek; Tomovska, R.

    2014-01-01

    Roč. 564, AUG 1 (2014), s. 115-120 ISSN 0040-6090 Grant - others:NATO SfP(US) 984399 Institutional support: RVO:67985858 Keywords : laser ablation * surface-enhanced raman scattering * nanocomposite s * graphene * rhodamine 6G Subject RIV: CH - Nuclear ; Quantum Chemistry Impact factor: 1.759, year: 2014

  6. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Highly sensitive colorimetric chemosensor molecule RHN for selective detection of Cu2+ in mixed CH3CN aqueous media was designed and prepared by incorporating the well-known rhodamine fluorophore and a terdentate O2N binding unit into one molecule. The chemosensor RHN showed not only a reversible, ...

  7. Single mode solid state distributed feedback dye laser fabricated by grey scale electron beam lithography on dye doped SU-8 resist

    DEFF Research Database (Denmark)

    Balslev, Søren; Rasmussen, Torben; Shi, Peixiong

    2005-01-01

    We demonstrate grey scale electron beam lithography on functionalized SU-8 resist for fabrication of single mode solid state dye laser devices. The resist is doped with Rhodamine 6G perchlorate and the lasers are based on a first order Bragg grating distributed feedback resonator. The lasers...

  8. A novel device for hazardous substances degradation based on double-cavitating-jets impingement: Parameters optimization and efficiency assessment.

    Science.gov (United States)

    Tao, Yuequn; Cai, Jun; Huai, Xiulan; Liu, Bin

    2017-08-05

    Hydrodynamic cavitation is an effective advanced oxidation process. But sometimes it cannot obtain satisfactory treatment efficiency by using hydrodynamic cavitation individually, so it is necessary to introduce intensive methods. Based on double-cavitating-jets impingement, this paper presents a novel device that has advantages of strong heat and mass transfer and efficient chemical reactions. Based on the device, a series of experimental investigations on degradation of a basic dye, i.e. Rhodamine B were carried out. Significant Rhodamine B removal from aqueous solution was observed during 2h treatment and the degradation reaction conformed to pseudo-first-order kinetics. The synergetic effects between double-cavitating-jets impingement and Fenton chemistry on simultaneous degradation of Rhodamine B were confirmed. Both single-variable experiments and orthogonal experiments were carried out to study the effects of initial hydrogen peroxide, ferrous sulfate and Rhodamine B concentrations and the optimum conditions were found out. Effects of jet inlet pressure in the range of 6-12MPa and solution pH value in the range of 2-8 were also investigated. The cavitation yield was evaluated to assess the energy efficiency. The present treatment scheme showed advantages in terms of reducing the demand of hydrogen peroxide concentration and enhancing the treatment efficiency in large scale operation. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Determination of antimony trioxide in fire-retardant conveyor belts

    Energy Technology Data Exchange (ETDEWEB)

    Rytych-Witwicka, B.; Szmyd, E.

    1976-12-01

    Two methods for the determination of antimony trioxide in rubber and pvc are described. One is a colorimetric method based on the reaction of antimony with rhodamine B; the other is a polarographic method. The results of the two methods show a satisfactory consistency and the methods themselves appear rapid and effective.

  10. Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging.

    Science.gov (United States)

    Nakamura, Akinobu; Takigawa, Kazumasa; Kurishita, Yasutaka; Kuwata, Keiko; Ishida, Manabu; Shimoda, Yasushi; Hamachi, Itaru; Tsukiji, Shinya

    2014-06-11

    We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.

  11. Ciprofloxacin@SiO2: Fluorescent nanobubbles

    Indian Academy of Sciences (India)

    Unknown

    Centre, Indian Institute of Technology Madras, Chennai 600 036, India ... modified pharmacological and toxicological properties of these drugs in the form of ... Apart from these, there are a few other studies on the application of the .... kind of red shift was also found in the case of rhodamine 6G adsorbed on clay minerals.

  12. Screening of thermophilic neutral lipase-producing Pseudomonas ...

    African Journals Online (AJOL)

    From oil-contaminated soil, three lipase-producing microorganisms were selected as good lipase producers using rhodamine B-olive oil plate agar and they were identified as from Pseudomonas, Burkholderia and Klebsiella genera by morphology, biochemical characterization and 16S rRNA gene sequencing. Among the ...

  13. A highly selective and sensitive "turn-on" fluorescence chemodosimeter for the detection of mustard gas.

    Science.gov (United States)

    Raghavender Goud, D; Purohit, Ajay Kumar; Tak, Vijay; Dubey, Devendra Kumar; Kumar, Pravin; Pardasani, Deepak

    2014-10-21

    A new chemodosimetric protocol based on a tandem S-alkylation followed by desulfurisation reaction of rhodamine-thioamide with mustard gas is reported. The chemodosimeter is highly selective for potential DNA alkylating agents like sulfur mustard, over other simple alkyl halides with the limit of detection of 4.75 μM.

  14. The antifungal effect of peptides from hymenoptera venom and their analogs

    Czech Academy of Sciences Publication Activity Database

    Slaninová, Jiřina; Putnová, Helena; Borovičková, Lenka; Šácha, Pavel; Čeřovský, Václav; Monincová, Lenka; Fučík, Vladimír

    2011-01-01

    Roč. 6, č. 2 (2011), s. 150-159 ISSN 1895-104X R&D Projects: GA ČR GA203/08/0536 Institutional research plan: CEZ:AV0Z40550506 Keywords : antifungal agents * candida * fluorescent microscopy * rhodamine 6G * fluorescein-labeled peptide Subject RIV: CC - Organic Chemistry Impact factor: 1.000, year: 2011

  15. Fluorescence lifetime of emitters with broad homogeneous linewidths modified in opal photonic crystals

    DEFF Research Database (Denmark)

    Nikolaev, Ivan S.; Lodahl, Peter; Vos, Willem L.

    2008-01-01

    We have investigated the dynamics of spontaneous emission from dye molecules embedded in opal photonic crystals. Fluorescence lifetimes of Rhodamine 6G (R6G) dye were measured as a function of both optical frequency and crystal lattice parameter of the polystyrene opals. Due to the broad...

  16. Nanoporous materials modified with biodegradable polymers as models for drug delivery applications

    DEFF Research Database (Denmark)

    Gruber, Mathias F; Schulte, Lars; Ndoni, Sokol

    2013-01-01

    of principle for a system combining these two encapsulation methods and consisting of a nanoporous polymer (NP) with the pores filled with a degradable polymer mixed with a drug model. Rhodamine 6G (R6G) mixed with Poly(l-Lactic Acid) (PLLA) were confined within the 14nm pores of a NP with gyroid morphology...

  17. Direct laser writing for nanoporous liquid core laser sensors

    DEFF Research Database (Denmark)

    Grossmann, Tobias; Christiansen, Mads Brøkner; Peterson, Jeffrey

    2012-01-01

    We report the fabrication of nanoporous liquid core lasers via direct laser writing based on two-photon absorption in combination with thiolene-chemistry. As gain medium Rhodamine 6G was embedded in the nanoporous polybutadiene matrix. The lasing devices with thresholds of 19 µJ/mm2 were measured...

  18. Intracellular Environment-Responsive Stabilization of Polymer Vesicles Formed from Head-Tail Type Polycations Composed of a Polyamidoamine Dendron and Poly(L-lysine

    Directory of Open Access Journals (Sweden)

    Kenji Kono

    2013-09-01

    Full Text Available For the development of effective drug carriers, nanocapsules that respond to micro-environmental changes including a decrease in pH and a reductive environment were prepared by the stabilization of polymer vesicles formed from head-tail type polycations, composed of a polyamidoamine dendron head and a poly(L-lysine tail (PAMAM dendron-PLL, through the introduction of disulfide bonds between the PLL tails. Disulfide bonds were successfully introduced through the reaction of Lys residues in the PAMAM dendron-PLL polymer vesicles with 2-iminothiolane. The stabilization of PAMAM dendron-PLL polymer vesicles was confirmed by dynamic light scattering measurements. In acid-base titration experiments, nanocapsules cross-linked by disulfide bonds had a buffering effect during the cellular uptake process. The PAMAM dendron-PLL nanocapsules were used to incorporate the fluorescent dyes rhodamine 6G and fluorescein as a drug model. Cationic rhodamine 6G was generally not released from the nanocapsules because of the electrostatic barrier of the PLL membrane. However, the nanocapsules were destabilized at high glutathione concentrations corresponding to intracellular concentrations. Rhodamine 6G was immediately released from the nanocapsules because of destabilization upon the cleavage of disulfide bonds. This release of rhodamine 6G from the nanocapsules was also observed in HeLa cells by laser confocal microscopy.

  19. S Ananda

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science. S Ananda. Articles written in Bulletin of Materials Science. Volume 29 Issue 5 October 2006 pp 433-438 Ceramics and Glasses. Photocatalytic degradation of rhodamine B dye using hydrothermally synthesized ZnO · K Byrappa A K Subramani S Ananda K M Lokanatha Rai R ...

  20. Study of the Leacril Dyeing Process by a Cationic Dye from an Emulsion System.

    Science.gov (United States)

    Chibowski, E.; Ortega, A. Ontiveros; Espinosa-Jiménez, M.; Perea-Carpio, R.; Holysz, L.

    2001-03-15

    Adsorption studies of a cationic dye, Rhodamine B, from an emulsion phase on Leacril fabric at different temperatures were conducted. The emulsion phase consisted of n-hexadecane emulsified by isopropyl alcohol (1 M) and stabilized by tannic acid. In the alcohol solution Rhodamine B was dissolved. The kinetics of its adsorption and desorption is discussed. The changes in Leacril surface free energy components in the dyeing process were also determined. The adsorption data show that the presence of an emulsion increases the dye adsorption at room temperature (293 K) and at 313 K, while at 333 K it is smaller than that from Rhodamine solution alone. However, Rhodamine desorbs more when adsorbed from the solution. Surface free energy components differ for the Leacril samples dyed at different temperatures, and the most hydrophobic surface was obtained for the samples dyed at 333 K, where the electron-donor component is the lowest one. In general, the work of water spreading is close to zero, except for the above sample for which it is relatively highly negative. Possible mechanisms of the dye adsorption are discussed. Copyright 2001 Academic Press.

  1. Gum karaya based hydrogel nanocomposites for the effective removal of cationic dyes from aqueous solutions

    CSIR Research Space (South Africa)

    Mittala, H

    2016-02-01

    Full Text Available after nanocomposite formation with SiC NPs. The HNC was employed for the removal of cationic dyes, i.e., malachite green (MG) and rhodamine B (RhB) from the aqueous solution. The HNC was found to remove 91% (MG) and 86% (RhB) of dyes with a polymer dose...

  2. The drug-binding activity of the multidrug-responding transcriptional regulator BmrR resides in its C-terminal domain.

    OpenAIRE

    Markham, P N; Ahmed, M; Neyfakh, A A

    1996-01-01

    Rhodamine and tetraphenylphosphonium, the substrates of the Bacillus subtilis multidrug efflux transporter Bmr, induce the expression of Bmr through direct interaction with its transcriptional activator BmrR. Here we show that the C-terminal domain of BmrR, expressed individually, binds both these compounds and therefore can be used as a model for molecular analysis of the phenomenon of multidrug recognition.

  3. Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation

    CSIR Research Space (South Africa)

    Visser, Daniel F

    2006-10-01

    Full Text Available : Olive Oil / Rhodamine B Tributyrin Fluorescence under UV Secondary Screening Colorimet- ric - PNP-ester substrates (C2, C4, C18); Substrate, Temperature and pH Profiling 16S ribosomal RNA gene (DNA level) Amidase ATP-dependant protease Beta...

  4. advances and problems in mathematical modelling of dispersion of ...

    African Journals Online (AJOL)

    ES Obe

    1981-03-01

    Mar 1, 1981 ... NIJOTECH VOL. 5 NO. 1 MARCH 1981. OJIAKO. 22 kt. D tux e. tD. Co. txC t. L. L. +. -. -. -. = 4. ) (. 2. ),(. 2 π. (29) k may, be determined from comparison of tracer profile and computer simmulation technique. k was studied using experimental channel runs of dye (Rhodamine B) tracer and well sieved organic.

  5. Graphene Oxide Based Nanocarrier Combined with a pH-Sensitive Tracer: A Vehicle for Concurrent pH Sensing and pH-Responsive Oligonucleotide Delivery.

    Science.gov (United States)

    Hsieh, Chia-Jung; Chen, Yu-Cheng; Hsieh, Pei-Ying; Liu, Shi-Rong; Wu, Shu-Pao; Hsieh, You-Zung; Hsu, Hsin-Yun

    2015-06-03

    We chemically tuned the oxidation status of graphene oxide (GO) and constructed a GO-based nanoplatform combined with a pH-sensitive fluorescence tracer that is designed for both pH sensing and pH-responsive drug delivery. A series of GOs oxidized to distinct degrees were examined to optimize the adsorption of the model drug, poly dT30. We determined that highly oxidized GO was a superior drug-carrier candidate in vitro when compared to GOs oxidized to lesser degrees. In the cell experiment, the synthesized pH-sensitive rhodamine dye was first applied to monitor cellular pH; under acidic conditions, protonated rhodamine fluoresces at 588 nm (λex=561 nm). When the dT30-GO nanocarrier was introduced into cells, a rhodamine-triggered competition reaction occurred, and this led to the release of the oligonucleotides and the quenching of rhodamine fluorescence by GO. Our results indicate high drug loading (FAM-dT30/GO=25/50 μg/mL) and rapid cellular uptake (<0.5 h) of the nanocarrier which can potentially be used for targeted RNAi delivery to the acidic milieu of tumors.

  6. Photoelectrochemical detection of toxic heavy metals

    CSIR Research Space (South Africa)

    Chamier, J

    2010-09-01

    Full Text Available on various substrates introduced the possibility for portable and on-site instant verification of heavy metal pollutants. In this work, the favorable properties of the mercury-sensitive fluorescent molecule, Rhodamine 6G hydrozone derivative (RS), were...

  7. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Role of vanadium and pyridine in heteropolycompounds for selective oxidation of ... in the selective oxidation of aromatic methyl group to aldehyde without mediators .... ion recognition studies of lower rim 1,3-di{rhodamine} conjugate of calix[4]arene ... Two expedient one-pot methods have been developed for synthesis of ...

  8. PREPARATION AND CHARACTERIZATION OF MOLECULARLY IMPRINTED ELECTROPOLYMERIZED CARBON ELECTRODES

    Science.gov (United States)

    Molecularly imprinted polymers (MIP) selective for fluorescein, rhodamine or 2,4-dichlorophenoxyacetic acid (2,4-D) were electropolymerized onto graphite electrodes using an aqueous solution equimolar in resorsinol/ortho-phenylenediamine and in the presence of the template mole...

  9. Levitated droplet dye laser

    DEFF Research Database (Denmark)

    Azzouz, H.; Alkafadiji, L.; Balslev, Søren

    2006-01-01

    a high quality optical resonator. Our 750 nL lasing droplets consist of Rhodamine 6G dissolved in ethylene glycol, at a concentration of 0.02 M. The droplets are optically pumped at 532 nm light from a pulsed, frequency doubled Nd:YAG laser, and the dye laser emission is analyzed by a fixed grating...

  10. Facile preparation of C, N co-modified Nb2O5 nanoneedles with enhanced visible light photocatalytic activity

    KAUST Repository

    Xue, Jiao; Wang, Runwei; Zhang, Zongtao; Qiu, Shilun

    2016-01-01

    activity than commercial Degussa P25 and commercial Nb2O5 towards photodegradation of Rhodamine B (RhB) at a concentration of 10 mg L−1 under visible light. Special chemical species, such as carbonate species and NOX species, that exist on the surface

  11. Proton Motive Force-Driven and ATP-Dependent Drug Extrusion Systems in Multidrug-Resistant Lactococcus lactis

    NARCIS (Netherlands)

    BOLHUIS, H; MOLENAAR, D; POELARENDS, G; VANVEEN, HW; POOLMAN, B; DRIESSEN, AJM; KONINGS, WN

    1994-01-01

    Three mutants of Lactococcus lactis subsp. lactis MG1363, termed Eth(R), Dau(R), and Rho(R), were selected for resistance to high concentrations of ethidium bromide, daunomycin, and rhodamine 6G, respectively. These mutants were found to be cross resistant to a number of structurally and

  12. Role of bismuth in nano-structured doped TiO2 photocatalyst prepared by environmentally benign soft synthesis

    Czech Academy of Sciences Publication Activity Database

    Henych, Jiří; Štengl, Václav; Kormunda, M.; Mattsson, A.; Österlund, L.

    2014-01-01

    Roč. 49, č. 9 (2014), s. 3560-3571 ISSN 0022-2461 Institutional support: RVO:61388980 Keywords : TITANIUM-DIOXIDE * RHODAMINE-B * RAMAN-SPECTROSCOPY * AQUEOUS-SOLUTION * SOLAR-CELL Subject RIV: CA - Inorganic Chemistry Impact factor: 2.371, year: 2014

  13. Photogalvanic cell: A new approach for green and sustainable chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Genwa, K.R. [Department of Chemistry, JNV University, Jodhpur 342 001 (India); Genwa, Mahaveer [Department of Chemistry, Deen Dayal Upadhyaya College (Delhi University), Shivaji Marg, Karam Pura, New Delhi 110015 (India)

    2008-05-15

    A comparative study of anionic, cationic, and nonionic surfactants on photogalvanic effect was studied in a photogalvanic cell containing dioctyl sulfosuccinate (DSS), cetyltrimethylammonium bromide (CTAB), and TritonX-100 as surfactants, rhodamine 6G as photosensitizer, and oxalic acid as reductant. Observed values of photopotential, photocurrent, fill factor, conversion efficiency, and storage capacity for DSS-rhodamine 6G-oxalic acid system was, respectively, 880.0 mV, 200 {mu}A, 0.41, 0.86%, and 131.0 min, for CTAB-rhodamine 6G-oxalic acid system, 414.0 mV, 90.0 {mu}A, 0.45, 0.24%, and 68.0 min, and for TritonX-100-rhodamine 6G-oxalic acid system, 672.0 mV, 165.0 {mu}A, 0.38, 0.55%, and 96.0 min. The effects of different parameters on electrical output of the cell were observed and a tentative mechanism has also been proposed for the generation of photocurrent in photogalvanic cell. (author)

  14. Gallium determination with Rodamina B: a simple method

    International Nuclear Information System (INIS)

    Queiroz, R.R.U. de.

    1981-01-01

    A simple method for determining gallium with Rhodamine B, by the modification of the method proposed by Onishi and Sandell. The complex (RH) GaCl 4 is extracted with a mixture benzene-ethylacetate (3:1 V/V), from an aqueous medium 6 M in hydrochloric acid. The interference of foreign ions is studied. (C.G.C.) [pt

  15. A functional assay for detection of the mitoxantrone resistance protein, MXR (ABCG2)

    DEFF Research Database (Denmark)

    Robey, R W; Honjo, Y; van de Laar, A

    2001-01-01

    The fluorescent compounds rhodamine 123, LysoTracker Green DMD-26, mitoxantrone, and BODIPY-prazosin were used with the antagonist fumitremorgin C (FTC) in order to develop functional assays for the half-transporter, MXR/BCRP/ABCP1. A measure of FTC-inhibitable efflux was generated for each compo...

  16. Diatomaceous Fungal and Bacterial Building Blocks for Material Synthesis

    Science.gov (United States)

    2008-04-08

    Thalassiosira-templated metallic microshells. Thalassiosira, only 1mM rhodamine 6G (d) lmM, (e) I piM ,(f 100 nM R6G. 4 concentrations gave detectable...hybridized with a 27-mer 7 oligonucleotide containing a 7-mer TAG GAA TAG TTA TA AT OTT ATT AGO complementary region 2, which produces TAIOATCCTTA TACAATAATCC

  17. Facile synthesis and photocatalytic activity of zinc oxide hierarchical microcrystals

    KAUST Repository

    Xu, Xinjiang; Kuang, Fangcheng; Xu, Jiangping

    2013-01-01

    was suggested. Furthermore, the catalytic activity of the ZnO microcrystals was studied by treating low concentration Rhodamine B (RhB) solution under UV light, and research results show the hierarchical microstructures of ZnO display high catalytic activity

  18. Combined nanoimprint and photolithography of integrated polymer optics

    DEFF Research Database (Denmark)

    Christiansen, Mads Brøkner; Schøler, Mikkel; Gersborg-Hansen, Morten

    2007-01-01

    are defined in SU-8 resist, doped with Rhodamine 6G laser dye, shaped as planar slab waveguides on a Fused Silica buffer substrate, and with a lst-order DFB surface corrugation forming the laser resonator. When optically pumped at 532 nm, lasing is obtained in the wavelength range 560 nm -600 nm, determined...

  19. Evaluation of one-step luminescent cyanoacrylate fuming.

    Science.gov (United States)

    Khuu, Alicia; Chadwick, Scott; Spindler, Xanthe; Lam, Rolanda; Moret, Sébastien; Roux, Claude

    2016-06-01

    One-step luminescent cyanoacrylates have recently been introduced as an alternative to the conventional cyanoacrylate fuming methods. These new techniques do not require the application of a luminescent post-treatment in order to enhance cyanoacrylate-developed fingermarks. In this study, three one-step polymer cyanoacrylates: CN Yellow Crystals (Aneval Inc.), PolyCyano UV (Foster+Freeman Ltd.) and PECA Multiband (BVDA), and one monomer cyanoacrylate: Lumikit™ (Crime Scene Technology), were evaluated against a conventional two-step cyanoacrylate fuming method (Cyanobloom (Foster+Freeman Ltd.) with rhodamine 6G stain). The manufacturers' recommended conditions or conditions compatible with the MVC™ 1000/D (Foster+Freeman Ltd.) were assessed with fingermarks aged for up to 8 weeks on non-porous and semi-porous substrates. Under white light, Cyanobloom generally gave better development than the one-step treatments across the substrates. Similarly when viewed under the respective luminescent conditions, Cyanobloom with rhodamine 6G stain resulted in improved contrast against the one-step treatments except on polystyrene, where PolyCyano UV and PECA Multiband gave better visualisation. Rhodamine 6G post-treatment of one-step samples did not significantly enhance the contrast of any of the one-step treatments against Cyanobloom/rhodamine 6G-treated samples. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  20. Chemically driven switches for online detection of pH changes in microfluidic devices

    NARCIS (Netherlands)

    Mela, P.; Onclin, S.; Goedbloed, M.H.; Levi, S.; Levi, S.A.; van Hulst, N.F.; van den Berg, Albert

    2003-01-01

    The internal walls of microfabricated fluidic channels were functionalized with a selfassembled monolayer of Rhodamine B lactam. This molecule has the capability to interconvert between its open fluorescent amide form and the closed non-fluorescent lactam form upon changes of the pH conditions. The