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Sample records for rhodamine 6g dye

  1. Fluorescence fluctuation of Rhodamine 6G dye for high repetition rate laser excitation

    International Nuclear Information System (INIS)

    Singh, Nageshwar; Patel, Hemant K.; Dixit, S.K.; Vora, H.S.

    2013-01-01

    In this paper, fluorescence from Rhodamine 6G dye for stationary and flowing liquid medium, excited by copper vapor laser, operating at 6 kHz pulse repetition frequency, was investigated. Large fluctuations in spectral width (about 5 nm) and spectral intensity in the fluorescence from stationary dye solution were observed, while fluctuations in the spectral width diminish in a flowing dye medium. However, this increases spectral intensity and slightly red shifts the fluorescence peak emission wavelength. Theoretical analysis was carried out to explain the observed results by incorporating the temperature induced refractive index, beam deflection and spectral variation in stationary dye solution. Numerical analysis of thermal load and contour of temperature in the optical pumped region inside the dye cell in stationary, 0.2 and 1.5 m/s flow velocity was also investigated to support our analysis. - Highlights: ► High repetition rate excitation generates inhomogeneity in the gain medium. ► Fluorescence of Rhodamine 6G in stationary and flowing medium was carried out. ► Fluorescence fluctuations lessen in flowing medium in contrast to stationary medium. ► Our theoretical and numerical analysis enlightens the experimented outcome trend.

  2. Biochemical activity of a fluorescent dye rhodamine 6G: Molecular modeling, electrochemical, spectroscopic and thermodynamic studies.

    Science.gov (United States)

    Al Masum, Abdulla; Chakraborty, Maharudra; Ghosh, Soumen; Laha, Dipranjan; Karmakar, Parimal; Islam, Md Maidul; Mukhopadhyay, Subrata

    2016-11-01

    Interaction of CT DNA with Rhodamine 6G (R6G) has been studied using molecular docking, electrochemical, spectroscopic and thermodynamic methods. From the study, it was illustrated that Rhodamine 6G binds to the minor groove of CT DNA. The binding was cooperative in nature. Circular voltametric study showed significant change in peak current and peak potential due to complexation. All the studies showed that the binding constant was in the order of 10 6 M -1 . Circular dichroic spectra showed significant conformational change on binding and DNA unwind during binding. Thermodynamic study showed that binding was favored by negative enthalpy and positive entropy change. From thermodynamic study it was also observed that several positive and negative free energies played significant role during binding and the unfavorable conformational free energy change was overcame by highly negative hydrophobic and salt dependent free energy changes. The experimental results were further validated using molecular docking study and the effect of structure on binding has been studied theoretically. From docking study it was found that the hydrophobic interaction and hydrogen bonds played a significant role during binding. The dye was absorbed by cell and this phenomenon was studied using fluorescent microscope. Cell survivability test showed that the dye active against Human Breast Cancer cells MDA-MB 468. ROS study showed that the activity is due to the production of reactive oxygen. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Degradation of a cationic dye (Rhodamine 6G) using hydrodynamic cavitation coupled with other oxidative agents: Reaction mechanism and pathway.

    Science.gov (United States)

    Rajoriya, Sunil; Bargole, Swapnil; Saharan, Virendra Kumar

    2017-01-01

    In the present study, decolorization and mineralization of a cationic dye, Rhodamine 6G (Rh6G), has been carried out using hydrodynamic cavitation (HC). Two cavitating devices such as slit and circular venturi were used to generate cavitation in HC reactor. The process parameters such as initial dye concentration, solution pH, operating inlet pressure, and cavitation number were investigated in detail to evaluate their effects on the decolorization efficiency of Rh6G. Decolorization of Rh6G was marginally higher in the case of slit venturi as compared to circular venturi. The kinetic study showed that decolorization and mineralization of the dye fitted first-order kinetics. The loadings of H 2 O 2 and ozone have been optimized to intensify the decolorization and mineralization efficiency of Rh6G using HC. Nearly 54% decolorization of Rh6G was obtained using a combination of HC and H 2 O 2 at a dye to H 2 O 2 molar ratio of 1:30. The combination of HC with ozone resulted in 100% decolorization in almost 5-10min of processing time depending upon the initial dye concentration. To quantify the extent of mineralization, total organic carbon (TOC) analysis was also performed using various processes and almost 84% TOC removal was obtained using HC coupled with 3g/h of ozone. The degradation by-products formed during the complete degradation process were qualitatively identified by liquid chromatography-mass spectrometry (LC-MS) and a detailed degradation pathway has been proposed. Copyright © 2016 Elsevier B.V. All rights reserved.

  4. Superradiant laser emission from rhodamine 6G and rhodamine B using a coaxial flashpump.

    Science.gov (United States)

    Mumola, P. B.

    1972-01-01

    Superradiant laser emission has been observed from ethanol solutions of rhodamine 6G and rhodamine B using a coaxial flashlamp pump. The dependence of input threshold energy on dye concentration is examined. The possibility of exciting other dyes to superradiance is discussed.

  5. Rhodamine 6G impregnated porous silica: A photoluminescence study

    Energy Technology Data Exchange (ETDEWEB)

    Anedda, A. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Canada) (Italy); Carbonaro, C.M. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Canada) (Italy)]. E-mail: cm.carbonaro@dsf.unica.it; Clemente, F. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy); Corpino, R. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy); Ricci, P.C. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy); Rossini, S. [Dipartimento di Fisica, Universita degli Studi di Cagliari and INMF UdR Cagliari, SP no8, Km 0700, 09042, Monserrato (Ca) (Italy)

    2005-12-15

    The optical properties of rhodamine 6G dye confined in porous silica are reported. Photoluminescence properties of embedded chromophores in mesoporous hosts can be affected by the surrounding matrices: shifts in emission spectra and variations of photoluminescence quantum yield are found as compared to dye solutions. Host-guest interactions are studied here by varying both SiO{sub 2} xerogels porosity and the dye concentration. Comparing samples obtained by impregnating matrices with 5.4 and 18.2 nm pores with solutions having concentrations in the rhodamine 6G high laser gain, matrices with 5.4 nm pores impregnated with a dye concentration of 5 x 10{sup -4} M are found to be the most stable and efficient in the examined range.

  6. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects.

    Science.gov (United States)

    Zehentbauer, Florian M; Moretto, Claudia; Stephen, Ryan; Thevar, Thangavel; Gilchrist, John R; Pokrajac, Dubravka; Richard, Katherine L; Kiefer, Johannes

    2014-01-01

    Rhodamine 6G (R6G), also known as Rhodamine 590, is one of the most frequently used dyes for application in dye lasers and as a fluorescence tracer, e.g., in the area of environmental hydraulics. Knowing the spectroscopic characteristics of the optical emission is key to obtaining high conversion efficiency and measurement accuracy, respectively. In this work, solvent and concentration effects are studied. A series of eight different organic solvents (methanol, ethanol, n-propanol, iso-propanol, n-butanol, n-pentanol, acetone, and dimethyl sulfoxide (DMSO)) are investigated at constant dye concentration. Relatively small changes of the fluorescence spectrum are observed for the different solvents; the highest fluorescence intensity is observed for methanol and lowest for DMSO. The shortest peak wavelength is found in methanol (568 nm) and the longest in DMSO (579 nm). Concentration effects in aqueous R6G solutions are studied over the full concentration range from the solubility limit to highly dilute states. Changing the dye concentration provides tunability between ∼550 nm in the dilute case and ∼620 nm at high concentration, at which point the fluorescence spectrum indicates the formation of R6G aggregates. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. Tunable cytotoxicity of rhodamine 6G via anion variations.

    Science.gov (United States)

    Magut, Paul K S; Das, Susmita; Fernand, Vivian E; Losso, Jack; McDonough, Karen; Naylor, Brittni M; Aggarwal, Sita; Warner, Isiah M

    2013-10-23

    Chemotherapeutic agents with low toxicity to normal tissues are a major goal in cancer research. In this regard, the therapeutic activities of cationic dyes, such as rhodamine 6G, toward cancer cells have been studied for decades with observed toxicities toward normal and cancer cells. Herein, we report rhodamine 6G-based organic salts with varying counteranions that are stable under physiological conditions, display excellent fluorescence photostability, and more importantly have tunable chemotherapeutic properties. Our in vitro studies indicate that the hydrophobic compounds of this series allow production of nanoparticles which are nontoxic to normal cells and toxic to cancer cells. Furthermore, the anions, in combination with cations such as sodium, were observed to be nontoxic to both normal and cancer cells. To the best of our knowledge, this is the first demonstration that both the cation and anion play an extremely important and cooperative role in the antitumor properties of these compounds.

  8. Fluorescence studies of Rhodamine 6G functionalized silicon oxide nanostructures

    International Nuclear Information System (INIS)

    Baumgaertel, Thomas; Borczyskowski, Christian von; Graaf, Harald

    2010-01-01

    Selective anchoring of optically active molecules on nanostructured surfaces is a promising step towards the creation of nanoscale devices with new functionalities. Recently we have demonstrated the electrostatic attachment of charged fluorescent molecules on silicon oxide nanostructures prepared by atomic force microscopy (AFM) nanolithography via local anodic oxidation (LAO) of dodecyl-terminated silicon. In this paper we report on our findings from a more detailed optical investigation of the bound dye Rhodamine 6G. High sensitivity optical wide field microscopy as well as confocal laser microscopy have been used to characterize the Rhodamine fluorescence emission. A highly interesting question concerns the interaction between an emitter close to a silicon surface because mechanisms such as energy transfer and fluorescence quenching will occur which are still not fully understood. Since the oxide thickness can be varied during preparation continuously from 1 to ∼ 5 nm, it is possible to investigate the fluorescence of the bound dye in close proximity to the underlying silicon. Using confocal laser microscopy we were also able to obtain optical spectra from the bound molecules. Together with the results from an analysis of their photochemical bleaching behaviour, we conjecture that some of the Rhodamine 6G molecules on the structure are interacting with the oxide, causing a spectral shift and differences in their photochemical properties.

  9. Fabrication and Photostability of Rhodamine-6G Gold Nanoparticle Doped Polymer Optical Fiber

    International Nuclear Information System (INIS)

    Sebastian, Suneetha; Ajina, C; Vallabhan, C. P. G; Nampoori, V. P. N.; Radhakrishnan, P.; Kailasnath, M.

    2013-01-01

    We report on fabrication of a rhodamine-6G-gold-nanoparticle doped polymer optical fiber. The gold nanoparticle is synthesized directly into the monomer solution of the polymer using laser ablation synthesis in liquid. The size of the particle is found from the transmission electron microscopy. Rhodamine-6G is then mixed with the nanoparticle-monomer solution and optical characterization of the solution is investigated. It is found that there is a pronounced quenching of fluorescence of rhodamine 6G due to fluorescence resonance energy transfer. The monomer solution containing rhodamine 6G and gold nanoparticles is now made into a cylindrical rod and drawn into a polymer optical fiber. Further, the photostability is calculated with respect to the pure dye doped polymer optical fiber

  10. Determination of Cerium (IV) Using Rhodamine 6G Fluorescence Quenching

    Science.gov (United States)

    Zhao, Zh.; Sheng, L.; Su, B.; Tao, C.; Jing, W.

    2017-11-01

    The interaction between rhodamine 6G (Rh6G) and cerium sulfate was studied by the fluorescence quenching method. In a sulfuric acid medium, the interaction of Ce(IV) with Rh6G results in Rh6G fluorescence quenching. The maximum excitation wavelength (λex) and the maximum emission wavelength (λem) are 530 nm and 555 nm, respectively. A good linearity between the relative fl uorescence intensity (ΔF) and Ce(IV) was observed in the range 0.12-1.08 μg/mL. The detection limit was 1.4 × 10-3 μg/mL. The optimum reaction conditions, influencing factors, and effect of coexisting substances were investigated in the experiment. We found that the concentration of Rh6G was 3.2 × 10-6 mol/L, and the fl uorescence intensity was maximum.

  11. Rhodamine 6G hexachloridostannate(IV acetonitrile disolvate

    Directory of Open Access Journals (Sweden)

    Ramaiyer Venkatraman

    2008-01-01

    Full Text Available In the title compound, bis({6-ethylamino-10-[2-(methoxycarbonylphenyl]-2,7-dimethylxanthen-3-ylidene}ethanaminium hexachloridotin(IV acetonitrile disolvate, (C27H29N2O32[SnCl6]·2C2H3N, the octahedral SnCl62− anion lies on an inversion center. The xanthene ring system is essentially planar, with an average deviation of 0.020 Å, and the substituent benzene ring forms a dihedral angle of 85.89 (2° with it. The Sn—Cl distances are in the range 2.4237 (3–2.4454 (3 Å. There are N—H...Cl hydrogen bonds between SnCl62− ions and rhodamine 6G cations as well as π–π stacking interactions between rhodamine 6G cations (interplanar distance of 3.827 Å.

  12. Silver nanoparticles plasmonic effect on eosin and rhodamine 6G luminescence in various media

    Science.gov (United States)

    Samusev, Ilia G.; Tikhomirova, Nadezhda S.; Slezhkin, Vasiliy A.; Zyubin, Andrey Yu.; Bryukhanov, Valery V.; Tsibulnikova, Anna V.

    2016-11-01

    The plasmonic enhancement and quenching of phosphorescence and fluorescence of the anionic (eosin) and cationic (rhodamine 6G) dyes have been studied in various environments: silver nanoparticles of silver hydrosol citrate in water, in polymer films and on the surface of nanoporous silica in order to determine the kinetic and spectral effects on the dye luminescence. Depending on the silver nanoparticles concentration both the enhancement and quenching of the dyes phosphorescence and fluorescence have been detected. The mechanism of interaction between the excited molecules and silver nanoparticles has been discussed.

  13. Extraction spectrophotometric determination of elements in form of ion-association compounds of metal chaltes and dyes. Pt. 2. The system cobalt-5, 7-dichloro-8-hydroxyquinoline-rhodamine 6G, benzene

    International Nuclear Information System (INIS)

    Minczewski, J.; Chwastowska, J.; Lachowicz, E.

    1976-01-01

    The extraction of the ion-pair formed by the anionic, coordinatively saturated complex of cobalt with 5,7-dichloro-8-hydroxyquinoline (Cl 2 HOx) and Rhodamine 6G cation (ROD + ) was investigated. The cobalt ions at the concentration 4 x 10 -5 M are fully extractable at pH=6.3; 1 x 10 -3 M Cl 2 HOx and 4 x 10 -4 M Rhodamine 6G after 10 minutes of extraction. In absence of Rhodamine 6G the cobalt complexes are not extractable. The extractable ion-pair has the composition according to the formula Co(Cl 2 Ox) 3 - .ROD + . The molar absorptivity at lambda=542 nm is equal to 7.5 x 10 4 . The extraction constant log Ksub(ex)=0.44+-0.14. The protonation constant of Rhodamine (log K=-0.98+-0.08) and two-phase stability constant of the carbinol base of Rhodamine 6G (log β = 5.35. +- 0.04) were also calculated. (author)

  14. Photostabilization of Rhodamine 6G in acetone by β-carotene and t-butyl hydroxyanisole

    OpenAIRE

    Aoki, Keisuke; Matsuda, Masahiro; Yamamoto, Daisuke; Abe, Yasuhiro; Akutsu, Shinya; Fujita, Koichi; Doi, Kazutaka; Kobayashi, Masami

    2011-01-01

    The photostability of Rhodamine 6G is of crucial importance for the organic light-emitting display of mobilephones. Photobleaching of Rhodamine 6G in acetone was not protected by β-carotene, but was efficientlyphotostabilized by t-butyl hydroxyanisole. The results indicate that photobleaching of Rhodamine 6G inacetone was not caused by singlet oxygen but some radical species.

  15. Low concentrations of Rhodamine-6G selectively destroy tumor cells and improve survival of melanoma transplanted mice.

    Science.gov (United States)

    Kutushov, M; Gorelik, O

    2013-01-01

    Rhodamine-6G is a fluorescent dye binding to mitochondria, thus reducing the intact mitochondria number and inhibiting mitochondrial metabolic activity. Resultantly, the respiratory chain functioning becomes blocked, the cell "suffocated" and eventually destroyed. Unlike normal cells, malignant cells demonstrate a priori reduced mitochondrial numbers and aberrant metabolism. Therefore, a turning point might exist, when Rhodamine-induced loss of active mitochondria would selectively destroy malignant, but spare normal cells. Various malignant vs. non-malignant cell lines were cultured with Rhodamine-6G at different concentrations. In addition, C57Bl mice were implanted with B16-F10 melanoma and treated with Rhodamine-6G at different dosage/time regimens. Viability and proliferation of cultured tumor cells were time and dose-dependently inhibited, up to 90%, by Rhodamine-6G, with profound histological signs of cell death. By contrast, inhibition of normal control cell proliferation hardly exceeded 15-17%. Melanoma-transplanted mice receiving Rhodamine-6G demonstrated prolonged survival, improved clinical parameters, inhibited tumor growth and metastases count, compared to their untreated counterparts. Twice-a-week 10-6M Rhodamine-6G regimen yielded the most prominent results. We conclude that malignant, but not normal, cells are selectively destroyed by low doses of Rhodamine-6G. In vivo, such treatment selectively suppresses tumor progression and dissemination, thus improving prognosis. We suggest that selective anti-tumor properties of Rhodamine-6G are based on unique physiologic differences in energy metabolism between malignant and normal cells. If found clinically relevant, low concentrations of Rhodamine-6G might be useful for replacing, or backing up, more aggressive nonselective chemotherapeutic compounds.

  16. Laser-Induced Population Inversion in Rhodamine 6G for Lysozyme Oligomer Detection.

    Science.gov (United States)

    Hanczyc, Piotr; Sznitko, Lech

    2017-06-06

    Fluorescence spectroscopy is a common method for detecting amyloid fibrils in which organic fluorophores are used as markers that exhibit an increase in quantum yield upon binding. However, most of the dyes exhibit enhanced emission only when bound to mature fibrils, and significantly weaker signals are obtained in the presence of amyloid oligomers. In the concept of population inversion, a laser is used as an excitation source to keep the major fraction of molecules in the excited state to create the pathways for the occurrence of stimulated emission. In the case of the proteins, the conformational changes lead to the self-ordering and thus different light scattering conditions that can influence the optical signatures of the generated light. Using this methodology, we show it is possible to optically detect amyloid oligomers using commonly available staining dyes in which population inversion can be induced. The results indicate that rhodamine 6G molecules are complexed with oligomers, and using a laser-assisted methodology, weakly emissive states can be detected. Significant spectral red-shifting of rhodamine 6G dispersed with amyloid oligomers and a notable difference determined by comparison of spectra of the fibrils suggest the existence of specific dye aggregates around the oligomer binding sites. This approach can provide new insights into intermediate oligomer states that are believed to be responsible for toxic seeding in neurodegeneration diseases.

  17. Fluorescence spectra of Rhodamine 6G for high fluence excitation laser radiation

    CERN Document Server

    Hung, J; Olaizola, A M

    2003-01-01

    Fluorescence spectral changes of Rhodamine 6G in ethanol and glycerol solutions and deposited as a film on a silica surface have been studied using a wide range of pumping field fluence at 532 nm at room temperature. Blue shift of the fluorescence spectra and fluorescence quenching of the dye molecule in solution are observed at high excitation fluence values. Such effects are not reported for the film sample. The effects are interpreted as the result of population redistribution in the solute-solvent molecular system induced by the high fluence field and the fluence dependence of the radiationless decay mechanism.

  18. Pulse radiolysis of ethanolic solutions of rhodamine dyes

    International Nuclear Information System (INIS)

    Kartasheva, L.I.; Kucherenko, E.A.; Kozlov, A.S.; Pikaev, A.K.

    1983-01-01

    The primary products of radiolytical transformations of rhodamine 6G, rhodamine B, rhodamine 3B and rhodamine 110 in ethanolic solutions were studied by pulse radiolysis method under various conditions. It was found that the semireduced form of a dye was the only intermediate product of such transformations in ethanolic solutions of all dyes. It was shown that this species was formed by interaction of the dye with esub(s) - and CH 3 CHOH. The properties of this species were investigated and the rate constants of respective reactions for each dye were determined. It was found that nature and position of a substituent in the molecule of the dye have an effect on the rate of formation of the semi-reduced form. (author)

  19. [Fluorescence Resonance Energy Transfer Detection of Cobalt Ions by Silver Triangular Nanoplates and Rhodamine 6G].

    Science.gov (United States)

    Zhang, Xiu-qing; Peng, Jun; Ling, Jian; Liu, Chao-juan; Cao, Qiu-e; Ding, Zhong-tao

    2015-04-01

    In the present paper, the authors studied fluorescence resonance energy transfer (FRET) phenomenon between silver triangular nanoplates and bovine serum albumin (BSA)/Rhodamine 6G fluorescence complex, and established a fluorescence method for the detection of cobalt ions. We found that when increasing the silver triangular nanoplates added to certain concentrations of fluorescent bovine serum albumin (BSA)/Rhodamine 6G complex, the fluorescence of Rhodamine 6G would be quenched up to 80% due to the FRET between the quencher and donor. However, in the presence of cobalt ions, the disassociation of the fluorescent complex from silver triangular nanoplates occurred and the fluorescence of the Rhodamine 6G recovered. The recovery of fluorescence intensity rate (I/I0) has a good relationship with the cobalt ion concentration (cCO2+) added. Thus, the authors developed a fluorescence method for the detection of cobalt ions based on the FRET of silver triangular nanoplates and Rhodamine 6G.

  20. Narrow Bandwidth Top-Emitting OLEDs Designed for Rhodamine 6G Excitation in Biological Sensing Applications

    Directory of Open Access Journals (Sweden)

    Matthias Jahnel

    2015-11-01

    Full Text Available Organic light emitting diodes (OLED are promising candidates offering in optical sensor applications to detect different gas compositions and excitable optical marker groups in chemical and biological processes. They enable attractive solutions for monitoring the gas phase composition of e.g., dissolved molecular oxygen (O2 species in bio reactors or excitation of fluorescent markers. In this work, we investigate different OLED devices for biomedical applications to excite the fluorescent dye rhodamine 6G (R6G. The OLED devices are built in top emission geometry comprising a distributed Bragg reflector (DBR acting as optical mirror. The OLED is optimized to provide a very narrow emission characteristic to excite the R6G at 530 nm wavelength and enabling the possibility to minimize the optical crosstalk between the OLED electroluminescence and the fluorescence of R6G. The DBR includes a thin film encapsulation and enables the narrowing of the spectral emission band depending on the number of DBR pairs. The comparison between optical simulation data and experimental results exhibits good agreement and proves process stability.

  1. Development of magnetic luminescent core/shell nanocomplex particles with fluorescence using Rhodamine 6G

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hee Uk; Song, Yoon Seok [Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701 (Korea, Republic of); Park, Chulhwan [Department of Chemical Engineering, Kwangwoon University, 447-1 Wolgye-Dong, Nowon-Gu, Seoul 139-701 (Korea, Republic of); Kim, Seung Wook, E-mail: kimsw@korea.ac.kr [Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701 (Korea, Republic of)

    2012-12-15

    Graphical abstract: Display Omitted Highlights: ► A simple method was developed to synthesize Co-B/SiO{sub 2}/dye/SiO{sub 2} composite particles. ► The magnetic particle shows that highly luminescent and core/shell particles are formed. ► Such core/shell particles can be easily suspended in water. ► The magnetic particles could detect fluorescence for the application of biosensor. -- Abstract: A simple and reproducible method was developed to synthesize a novel class of Co-B/SiO{sub 2}/dye/SiO{sub 2} composite core/shell particles. Using a single cobalt core, Rhodamine 6G of organic dye molecules was entrapped in a silica shell, resulting in core/shell particles of ∼200 nm diameter. Analyses using a variety of techniques such as transmission electron microscopy, X-ray photoelectron spectroscopy, vibration sample magnetometry, confocal laser scanning microscopy, and fluorescence intensity demonstrated that dye molecules were trapped inside the core/shell particles. A photoluminescence investigation showed that highly luminescent and photostable core/shell particles were formed. Such core/shell particles can be easily suspended in water. The synthesized magnetic particles could be used to detect fluorescence on glass substrate arrays for bioassay and biosensor applications.

  2. Rapid and simple preparation of rhodamine 6G loaded HY zeolite for highly selective nitrite detection

    Science.gov (United States)

    Viboonratanasri, Duangkamon; Pabchanda, Suwat; Prompinit, Panida

    2018-05-01

    In this study, a simple, rapid and relatively less toxic method for rhodamine 6G dye adsorption on hydrogen-form Y-type zeolite for highly selective nitrite detection was demonstrated. The adsorption behavior was described by Langmuir isotherm and the adsorption process reached the equilibrium promptly within a minute. The developed test papers characterized by fluorescence technique display high sensing performance with wide working range (0.04-20.0 mg L-1) and high selectivity. The test papers show good reproducibility with relative standard deviation (RSD) of 7% for five replicated determinations of 3 mg L-1 of nitrite. The nitrite concentration determined by using the test paper was in the same range as using ion chromatography within a 95% confidence level. The test papers offer advantages in terms of low cost and practical usage enabling them to be a promising candidate for nitrite sensor in environmental samples, food, and fertilizers.

  3. Efficient photocatalytic degradation of rhodamine 6G with a quantum dot-metal organic framework nanocomposite.

    Science.gov (United States)

    Kaur, Rajnish; Vellingiri, Kowsalya; Kim, Ki-Hyun; Paul, A K; Deep, Akash

    2016-07-01

    The hybrid structures of metal organic frameworks (MOFs) and nanoparticles may offer the realization of effective photocatalytic materials due to combined benefits of the porous and molecular sieving properties of MOF matrix and the functional characteristics of encapsulated nanoparticles. In this study, cadmium telluride (CdTe) quantum dots (QD) are conjugated with a europium-MOF for the synthesis of a novel nanocomposite material with photocatalytic properties. Successful synthesis of a QD/Eu-MOF nanocomposite was characterized with various spectroscopic and microscopic techniques. This QD/Eu-MOF is found to be an effective catalyst to complete the degradation of Rhodamine 6G dye within 50 min. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Quirks of dye nomenclature. 5. Rhodamines.

    Science.gov (United States)

    Cooksey, C J

    2016-01-01

    Rhodamines were first produced in the late 19(th) century, when they constituted a new class of synthetic dyes. These compounds since have been used to color many things including cosmetics, inks, textiles, and in some countries, food products. Certain rhodamine dyes also have been used to stain biological specimens and currently are widely used as fluorescent probes for mitochondria in living cells. The early history and current biological applications are sketched briefly and an account of the ambiguities, complications and confusions concerning dye identification and nomenclature are discussed.

  5. [Fluorescence Determination of Trace Se with the Hydride-K13-Rhodamine 6G System].

    Science.gov (United States)

    Liang, Ai-hui; Li, Yuan; Huang, Shan-shan; Luo, Yang-he; Wen, Gui-qing; Jiang, Zhi-liang

    2015-05-01

    Se is a necessary trace element for human and animals, but the excess intake of Se caused poison. Thus, it is very important to determination of Se in foods and water. The target of this study is development of a new, sensitive and selective hydride generation-molecular fluorescence method for the determination of Se. In 0. 36 mol . L-1 sulfuric acid, NaBH4 as reducing agent, Se (IV) is reduced to H2 Se. Usin3-g I solution as absorption liquid3, I- is reduced to I- by H2Se. When adding rhodamine 6G, Rhodamine 6G and I3- form association particles, which lead to the fluorescence intensity decreased. When Se(IV) existing, Rhodamine 6G and I3- bind less, And the remaining amount of Rhodamine 6G increase. So the fluorescence intensity is enhanced. The analytical conditions were optimized, a 0. 36 ml . L-1 H2SO4, 21. 6.g . L-1 NaBH4, 23.3 µm . L-1 rhodamine 6G, and 50 µmol . L-1 KI3 were chosen for use. When the excitation wavelength is at 480nm, the Rayleigh scattering peak does not affect the fluorescence recording, and was selected for determination of Se. Under the selected conditions, Se(IV) concentration in the 0. 02~0. 60 µg . mL-1 range and the increase value of the fluorescence intensity (ΔF) at 562 nm linear relationship. The linear regression equation is ΔF562 nm =12. 6c + 20. 9. The detecton limit was 0.01 µ.g . L-1. The influence of coexistence substances on the hydride generatin-molecular fluorescence determination of 5. 07 X10(-6) mol . L-1 Se(IV) was considered in details. Results showed that this new fluorescence method is of high selectivity, that is, 0. 5 mmol. L-1 Ba2+, Ca2+, Zn2+ and Fe3+, 0. 25 mmol . L-1 . Mg2+, 0. 05 mmol . L-1 K+, 0. 2 mmol . L-1 Al3+, 0. 025 mmol . L-1 Te(VI) do not interfere with the determination. The influence of Hg2+, CD2+ and Cu2+ that precipitate with Se(IV), can be eliminated by addition of complex reagent. This hydride generation-molecular fluorescence method has been applied to determination of trace Se in water

  6. Giant unilamellar vesicles containing Rhodamine 6G as a marker for immunoassay of bovine serum albumin and lipocalin-2.

    Science.gov (United States)

    Sakamoto, Misato; Shoji, Atsushi; Sugawara, Masao

    2016-07-15

    Functionalized giant unilamellar vesicles (GUVs) containing a fluorescence dye Rhodamine 6G is proposed as a marker in sandwich-type immunoassay for bovine serum albumin (BSA) and lipocalin-2 (LCN2). The GUVs were prepared by the electroformation method and functionalized with anti-BSA antibody and anti-LCN2 antibody, respectively. The purification of antibody-modified GUVs was achieved by conventional centrifugation and a washing step in a flow system. To antigen on an antibody slip, antibody-modified GUVs were added as a marker and incubated. After wash-out of excess reagents and lysis of the bound GUVs with Triton X-100, the fluorescence image was captured. The fluorometric immunoassays for BSA and LCN2 exhibited lower detection limits of 4 and 80 fg ml(-)(1), respectively. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. Enhanced chemiluminescence for trazodone trace analysis based on acidic permanganate oxidation in concurrent presence of rhodamine 6G.

    Science.gov (United States)

    Fujimori, Keiichi; Sakata, Yuta; Moriuchi-Kawakami, Takayo; Shibutani, Yasuhiko

    2017-11-01

    A new sensitized chemiluminescence method by acidic permanganate oxidation was developed for the sensitive determination of trazodone. A fluorescent dye as used rhodamine 6G to increase a chemiluminescence intensity. Under optimum conditions, the liner range of the calibration curve was obtained for 1-5000 nmol/L. The limit of detection was calculated from 3σ of a blank was 0.23 nmol/L. The coexistent ions and substances had no interference with the chemiluminescence measurement. The chemiluminescence spectra were measured to elucidate a possible mechanism for the system. The present method was satisfactorily used in the determination of the drugs in pharmaceutical samples and animal serums. Copyright © 2017 John Wiley & Sons, Ltd.

  8. Pulse radiolysis of rhodamine dye solutions

    International Nuclear Information System (INIS)

    Kucherenko, E.A.; Kartasheva, L.I.; Pikaev, A.K.

    1982-01-01

    Applying the method of pulse radiolysis (5 MeV electrons) a study was made on intermediate products of rhodamine B radiolytic transformations in neutral aqueous and ethanol solutions. Rate constants of reactions of esub(aq) and OH with the dye (they are equal to (2.2+-0.3)x10 10 and (2.1+-0.3)x10 10 e/molxs, accordingly) as well as optical and kinetic characteristics of esub(aq), OH and H interaction products were measured. The nature of these products is concluded. It was found that in ethanol solutions the semirecovered form - electroneutral radical of rhodamine B - was the only intermediate product. It arises during the interaction of the dye with esub(s) (k=(9.2+-1.2)x10 9 e/molxs) and α-et hananol radical (k=(1.1+-0.1)x10 8 l/molxs). Properties of this product were investigated

  9. Flotation-spectrophotometric determination of ruthenium in the Ru(IV)-chloride-rhodamine 6G-toluene system

    International Nuclear Information System (INIS)

    Balcerzak, M.

    1985-01-01

    The reduction of RuO 4 in hydrochloric acid has been examined. A sensitive flotation-spectrophotometric method of the determination of ruthenium based on the ion associate formed by the anionic chlorid complex of ruthenium RuCl 2- 6 with the basic dye Rhodamine 6G (R6G) has been developed. The solution of the ion associate obeys Beer's law up to the concentration of 0.25 μg Ru/ml. The ion associate precipitates when the aqueous solution is shaken with toluene. The separated compound is dissolved in acetone. The molar absorptivity (epsilon) at 530 nm is 5.1 x 10 5 l x mole -1 x cm -1 . The relative standard deviation is 3-7%. The mole ratio of Ru:R6G in the complex is 1:5. Osmium reacts similarly. The determination of ruthenium can be selective after the preliminary separation of osmium as OsO 4 . The method was applied to the determination of microgram amounts of ruthenium in crucible platinum. (Author)

  10. Photolysis of rhodamine-WT dye

    Science.gov (United States)

    Tai, D.Y.; Rathbun, R.E.

    1988-01-01

    Photolysis of rhodamine-WT dye under natural sunlight conditions was determined by measuring the loss of fluorescence as a function of time. Rate coefficients at 30?? north latitude ranged from 4.77 x 10-2 day-1 for summer to 3.16 x 10-2 day-1 for winter. Experimental coefficients were in good agreement with values calculated using a laboratory-determined value of the quantum yield.

  11. Cavity-enhanced spontaneous emission rates for rhodamine 6-G in levitated microdroplets

    International Nuclear Information System (INIS)

    Barnes, M.D.; Whitten, W.B.; Ramsey, J.M.; Arnold, S.

    1992-01-01

    Fluorescence decay kinetics of Rhodamine 6-G molecules in levitated glycerol microdroplets (4--20 microns in diameter) have been investigated to determine the effects of spherical cavity resonances on spontaneous emission rates. For droplet diameters greater than 10 microns, the fluorescence lifetime is essentially the same as in bulk glycerol. As the droplet diameter is decreased below 10 microns, bi-exponential decay behavior is observed with a slow component whose rate is similar to bulk glycerol, and a fast component whose rate is as much as a factor of 10 larger than the bulk decay rate. This fast component is attributed to cavity enhancement of the spontaneous emission rate and, within the weak coupling approximation, a value for the homogeneous linewidth at room temperature can be estimated from the fluorescence lifetime data

  12. Two rhodamine 6G derivative compounds: a structural and fluorescence single-crystal study.

    Science.gov (United States)

    Di Paolo, Matias; Bossi, Mariano L; Baggio, Ricardo; Suarez, Sebastián A

    2016-10-01

    The synthesis, characterization, structural analysis and fluorescence properties of two rhodamine 6G derivatives are described, namely a propargylamine derivative, 3',6'-bis(ethylamino)-2',7'-dimethyl-2-(methylcyanide)spiro[isoindole-1,9'-xanthen]-3(2H)-one (I), and a γ-aminobutyric acid (GABA) derivative, 3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindole-1,9'-xanthen]-2(3H)-yl)butyricacid (II). Both structures are compared with four similar ones from the Cambridge Structural Database (CSD), and the interactions involved in the stabilization are analyzed using the atoms in molecules (AIM) theory. Finally, a single-crystal in-situ reaction study is presented, carried out by fluorescence methods, which enabled the `opening' of the spirolactam ring in the solid phase.

  13. Tuning luminescence intensity of RHO6G dye using silver ...

    Indian Academy of Sciences (India)

    Wintec

    Wang and Kerker (1982) found that due to interaction of metal and dye in core shell particles splitting of extinction bands occurs. En- hancement also has been reported due to such interaction. Quenching of the luminescence of dye molecules ad- sorbed on a smooth Ag surface was observed by Ritchie and Burstein (1981).

  14. Random laser based on Rhodamine 6G (Rh6G doped poly(methyl methacrylate (PMMA films coating on ZnO nanorods synthesized by hydrothermal oxidation

    Directory of Open Access Journals (Sweden)

    Hua Zhang

    Full Text Available Random laser based on Rh6G doped PMMA thin films coating on ZnO nanorods synthesized by a simple hydrothermal oxidation method has been demonstrated. This kind of random laser medium is based on waveguide structure consisting of ZnO nanorods, Rh6G doped PMMA film and air. By controlling the time of hydrothermal oxidation reaction, wheat-like and hexagonal prism ZnO nanorods have been successfully fabricated. The emission spectra of these gain mediums based on different ZnO nanorods are different. The one based on wheat-like ZnO nanorods mainly exhibits amplified spontaneous emission, and the other one based on hexagonal prism ZnO nanorods shows random laser emission. The threshold of the random laser medium is about 73.8 μJ/pulse, and the full width at half maximum (FWHM is around 2.1 nm. The emission spectra measured at different detecting angles reveal that the output direction is strongly confined in ±30° by the waveguide effect. Our experiments demonstrate a promising method to achieve organic random laser medium. Keywords: Random laser, ZnO nanorods, Hydrothermal oxidation, Rhodamine 6G (Rh6G, Poly(methyl methacrylate (PMMA

  15. Lasing of a Solid-State Active Element Based on Anodized Aluminum Oxide Film Doped with Rhodamine 6G

    Science.gov (United States)

    Shelkovnikov, V. V.; Lyubas, G. A.; Korotaev, S. V.; Kopylova, T. N.; Tel'minov, E. N.; Gadirov, R. M.; Nikonova, E. N.; Nikonov, S. Yu.; Solodova, T. A.; Novikov, V. A.

    2017-04-01

    Spectral-luminescent and lasing characteristics of rhodamine 6G in porous aluminum oxide films anodized under various conditions are investigated. Lasing is obtained without external resonator in the longitudinal scheme under excitation by the second harmonic of Nd3+:YAG-laser radiation. The threshold pump power densities are in the range 3.5-15 MW/cm2 depending on the anodizing conditions. Wherein, the lasing line narrows down from 12 to 5 nm.

  16. Comparison and mechanism of photocatalytic activities of N-ZnO and N-ZrO2 for the degradation of rhodamine 6G.

    Science.gov (United States)

    Sudrajat, Hanggara; Babel, Sandhya

    2016-05-01

    N-doped ZnO (N-ZnO) and N-doped ZrO2 (N-ZrO2) are synthesized by novel, simple thermal decomposition methods. The catalysts are evaluated for the degradation of rhodamine 6G (R6G) under visible and UV light. N-ZnO exhibits higher dye degradation under both visible and UV light compared to N-ZrO2 due to possessing higher specific surface area, lower crystalline size, and lower band gap. However, it is less reusable than N-ZrO2 and its photocatalytic activity is also deteriorated at low pH. At the same intensity of 3.5 W/m(2), UVC light is shown to be a better UV source for N-ZnO, while UVA light is more suitable for N-ZrO2. At pH 7 with initial dye concentration of 10 mg/L, catalyst concentration of 1 g/L, and UVC light, 94.3 % of R6G is degraded by N-ZnO within 2 h. Using UVA light under identical experimental conditions, 93.5 % degradation of R6G is obtained by N-ZrO2. Moreover, the type of light source is found to determine the reactive species produced in the R6G degradation by N-ZnO and N-ZrO2. Less oxidative reactive species such as superoxide radical and singlet oxygen play a major role in the degradation of R6G under visible light. On the contrary, highly oxidative hydroxyl radicals are predominant under UVC light. Based on the kinetic study, the adsorption of R6G on the catalyst surface is found to be the controlling step.

  17. Rhodamine-WT dye losses in a mountain stream environment

    Science.gov (United States)

    Bencala, Kenneth E.; Rathburn, Ronald E.; Jackman, Alan P.; Kennedy, Vance C.; Zellweger, Gary W.; Avanzino, Ronald J.

    1983-01-01

    A significant fraction of rhodamine WT dye was lost during a short term multitracer injection experiment in a mountain stream environment. The conservative anion chloride and the sorbing cation lithium were concurrently injected. In-stream rhodamine WT concentrations were as low as 45 percent of that expected, based on chloride data. Concentration data were available from shallow‘wells’dug near the stream course and from a seep of suspected return flow. Both rhodamine WT dye and lithium were nonconservative with respect to the conservative chloride, with rhodamine WT dye closely following the behavior of the sorbing lithium.Nonsorption and sorption mechanisms for rhodamine WT loss in a mountain stream were evaluated in laboratory experiments. Experiments evaluating nonsorption losses indicated minimal losses by such mechanisms. Laboratory experiments using sand and gravel size streambed sediments show an appreciable capacity for rhodamine WT sorption.The detection of tracers in the shallow wells and seep indicates interaction between the stream and the flow in the surrounding subsurface, intergravel water, system. The injected tracers had ample opportunity for intimate contact with materials shown in the laboratory experiments to be potentially sorptive. It is suggested that in the study stream system, interaction with streambed gravel was a significant mechanism for the attenuation of rhodamine WT dye (relative to chloride).

  18. Mixed Hemi/Ad-Micelle Sodium Dodecyl Sulfate-Coated Magnetic Iron Oxide Nanoparticles for the Efficient Removal and Trace Determination of Rhodamine-B and Rhodamine-6G.

    Science.gov (United States)

    Ranjbari, Elias; Hadjmohammadi, Mohammad Reza; Kiekens, Filip; De Wael, Karolien

    2015-08-04

    Mixed hemi/ad-micelle sodium dodecyl sulfate (SDS)-coated magnetic iron oxide nanoparticles (MHAMS-MIONPs) were used as an efficient adsorbent for both removal and preconcentration of two important carcinogenic xanthine dyes named rhodamine-B (RB) and rhodamine-6G (RG). To gain insight in the configuration of SDS molecules on the surface of MIONPs, zeta potential measurements were performed in different [SDS]/[MIONP] ratios. Zeta potential data indicated that mixed hemi/ad-micelle MHAM was formed in [SDS]/[MIONP] ratios over the range of 1.1 to 7.3. Parameters affecting the adsorption of dyes were optimized as removal efficiency by one variable at-a-time and response surface methodology; the obtained removal efficiencies were ∼100%. Adsorption kinetic and equilibrium studies, under the optimum condition (pH = 2; amount of MIONPs = 87.15 mg; [SDS]/[MIONP] ratio = 2.9), showed that adsorption of both dyes are based on the pseudo-second-order and the Langmuir isotherm models, respectively. The maximum adsorption capacities for RB and RG were 385 and 323 mg g(-1), respectively. MHAMS-MIONPs were also applied for extraction of RB and RG. Under optimum conditions (pH = 2; amount of damped MHAMS-MIONPs = 90 mg; eluent solvent volume = 2.6 mL of 3% acetic acid in acetonitrile), extraction recoveries for 0.5 mg L(-1) of RB and RG were 98% and 99%, with preconcentration factors of 327 and 330, respectively. Limit of detection obtained for rhodamine dyes were <0.7 ng mL(-1). Finally, MHAMS-MIONPs were successfully applied for both removal and trace determination of RB and RG in environmental and wastewater samples.

  19. Application of derivative and derivative ratio spectrophotometry to simultaneous trace determination of rhodamine B and rhodamine 6G after dispersive liquid-liquid microextraction.

    Science.gov (United States)

    Xiao, Ni; Deng, Jian; Huang, Kaihui; Ju, Saiqin; Hu, Canhui; Liang, Jun

    2014-07-15

    Two novel methods, first derivative spectrophotometric method ((1)D) and first derivative ratio spectrophotometric method ((1)DR), have been developed for the simultaneous trace determination of rhodamine B (RhB) and rhodamine 6G (Rh6G) in food samples after dispersive liquid-liquid microextraction (DLLME). The combination of derivative spectrophotometric techniques and DLLME procedure endows the presented methods with enhanced sensitivity and selectivity. Under optimum conditions, the linear calibration curves ranged from 5 to 450 ng mL(-1), with the correlation coefficients (r) of 0.9997 for RhB and 0.9977 for Rh6G by (1)D method, and 0.9987 for RhB and 0.9958 for Rh6G by (1)DR method, respectively. The calculated limits of detection (LODs) based on the variability of the blank solutions (S/N = 3 criterion) for 11 measurements were in the range of 0.48-1.93 ng mL(-1). The recoveries ranged from 88.1% to 111.6% (with RSD less than 4.4%) and 91.5-110.5% (with RSD less than 4.7%) for (1)D and (1)DR method, respectively. The influence of interfering substances such as foreign ions and food colorants which might be present in the food samples on the signals of RhB and Rh6G was examined. The developed methods have been successfully applied to the determination of RhB and Rh6G in black tea, red wine and chilli powder samples with the characteristics of simplicity, cost-effectiveness, environmental friendliness, and could be valuable for routine analysis. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Photoionization of rhodamine dyes adsorbed at the aqueous solution surfaces investigated by synchrotron radiation

    International Nuclear Information System (INIS)

    Seno, Koichiro; Ishioka, Toshio; Harata, Akira; Hatano, Yoshihiko

    2002-01-01

    Photoionization spectroscopy using synchrotron radiation as a photon source was applied to the aqueous solution surfaces of rhodamine B (RhB), rhodamine 6G (Rh6G), and rhodamine 101 (Rh101) with their concentration of 0-100 μmol dm -3 . Synchrotron radiation was irradiated upon the solution surface between two electrodes in the photon energy range of 4-7 eV, and photoionization current was measured by a pico-ammeter. The photocurrent for each of the rhodamine aqueous solution surface showed an increase with the photon energy above a threshold photon energy. The photoionization threshold of RhB at the aqueous solution surface was 5.6 eV, which was smaller than that in the gas phase. The intensity of photoionization current of Rh6G was smaller than that of RhB or Rh101. Photoionization processes and the state of Rhodamine dye molecules at the aqueous solution surface were discussed in connection with results of surface tension measurements. (author)

  1. Polypyrrole-magnetite dispersive micro-solid-phase extraction combined with ultraviolet-visible spectrophotometry for the determination of rhodamine 6G and crystal violet in textile wastewater.

    Science.gov (United States)

    Kamaruddin, Amirah Farhan; Sanagi, Mohd Marsin; Wan Ibrahim, Wan Aini; Md Shukri, Dyia S; Abdul Keyon, Aemi S

    2017-11-01

    Polypyrrole-magnetite dispersive micro-solid-phase extraction method combined with ultraviolet-visible spectrophotometry was developed for the determination of selected cationic dyes in textile wastewater. Polypyrrole-magnetite was used as adsorbent due to its thermal stability, magnetic properties, and ability to adsorb Rhodamine 6G and crystal violet. Dispersive micro-solid-phase extraction parameters were optimized, including sample pH, adsorbent amount, extraction time, and desorption solvent. The optimum polypyrrole-magnetite dispersive micro-solid phase-extraction conditions were sample pH 8, 60 mg polypyrrole-magnetite adsorbent, 5 min of extraction time, and acetonitrile as the desorption solvent. Under the optimized conditions, the polypyrrole-magnetite dispersive micro-solid-phase extraction with ultraviolet-visible method showed good linearity in the range of 0.05-7 mg/L (R 2  > 0.9980). The method also showed a good limit of detection for the dyes (0.05 mg/L) and good analyte recoveries (97.4-111.3%) with relative standard deviations extraction and determination of dyes at trace concentration levels. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. Characteristics of a Broadband Dye Laser Using Pyrromethene and Rhodamine Dyes

    Science.gov (United States)

    Tedder, Sarah A.; Danehy, Paul M.; Wheeler, Jeffrey L.

    2011-01-01

    A broadband dye laser pumped by a frequency-doubled Nd:YAG laser with a full-width half-maximum (FWHM) from 592 to 610 nm was created for the use in a dual-pump broadband CARS system called WIDECARS. The desired broadband dye laser was generated with a mixture of Pyrromethene dyes as an oscillator gain medium and a spectral selective optic in the oscillator cavity. A mixture of Rhodamine dyes were used in the amplifier dye cell. To create this laser a study was performed to characterize the spectral behavior of broadband dye lasers created with Rhodamine dyes 590, 610, and 640, Pyrromethene dyes 597 and 650 as well as mixture of these dyes.

  3. Synthesis and study of the optical properties of dielectric Bragg reflectors infiltrated with 6G-Rhodamine

    International Nuclear Information System (INIS)

    Gómez-Barojas, E; Aca-López, V; Luna-López, J A; Sánchez-Mora, E; Silva-González, R

    2014-01-01

    We report the study of the optical properties of 6G-Rhodamine (Rhd) infiltrated porous silicon dielectric Bragg reflectors (DBRs) with 31 constituent periods. The DBRs were obtained by an electrochemical anodizing process of Si in a two electrodes Teflon cell. The porosity was determined by gravimetric measurements on single Porous silicon (PSi) layers. Based on the characterization results of single layers the DBRs were synthesized. After anodizing, the DBRs were silanized with a 3-mercaptopropyltrimethoxysilane solution and functionalized with Rhd solutions at different concentrations. Cross section scanning electron micrographs show that the DBRs synthesis was successful. After each preparation step, Reflectance and Fluorescence (FL) spectra were recorded. These spectra show that as the Rhd concentration in solution is increased the stop band intensity as well as the FL intensity are enhanced due to constructive interference effects

  4. LiFePO4 microcrystals as an efficient heterogeneous Fenton-like catalyst in degradation of rhodamine 6G.

    Science.gov (United States)

    Li, Zhan Jun; Ali, Ghafar; Kim, Hyun Jin; Yoo, Seong Ho; Cho, Sung Oh

    2014-01-01

    We present a novel heterogeneous Fenton-like catalyst of LiFePO4 (LFP). LFP has been widely used as an electrode material of a lithium ion battery, but we observed that commercial LFP (LFP-C) could act as a good Fenton-like catalyst to decompose rhodamine 6G. The catalytic activity of LFP-C microparticles was much higher than a popular catalyst, magnetite nanoparticles. Furthermore, we found that the catalytic activity of LFP-C could be further increased by increasing the specific surface area. The reaction rate constant of the hydrothermally synthesized LFP microcrystals (LFP-H) is at least 18 times higher than that of magnetite nanoparticles even though the particle size of LFP is far larger than magnetite nanoparticles. The LFP catalysts also exhibited a good recycling behavior and high stability under an oxidizing environment. The effects of the experimental parameters such as the concentration of the catalysts, pH, and the concentration of hydrogen peroxide on the catalytic activity of LFP were also analyzed.

  5. Comparitive study of fluorescence lifetime quenching of rhodamine 6G by MoS2 and Au-MoS2

    Science.gov (United States)

    Shakya, Jyoti; Kasana, Parath; Mohanty, T.

    2018-04-01

    Time resolved fluorescence study of Rhodamine 6G (R6G) in the presence of Molybdenum disulfide (MoS2) nanosheets and gold doped MoS2 (Au-MoS2) have been carried out and discussed. We have analyzed the fluorescence decay curves of R6G and it is observed that Au-MoS2 is a better fluorescence lifetime quencher as compare to MoS2 nanosheets. Also, the energy transfer efficiency and energy transfer rate from R6G to MoS2 and Au-MoS2 has been calculated and found higher for Au-MoS2.

  6. Growth temperature dependent surface plasmon resonances of densely packed gold nanoparticles’ films and their role in surface enhanced Raman scattering of Rhodamine6G

    International Nuclear Information System (INIS)

    Verma, Shweta; Rao, B. Tirumala; Bhartiya, S.; Sathe, V.; Kukreja, L.M.

    2015-01-01

    Highlights: • Growth temperature produces and tunes the surface plasmon resonance (SPR) of gold films. • Optimum thickness and growth temperature combination results narrow SPR band. • Alumina capping red-shifted the SPR band and showed marginal re-sputtering of films. • Densely packed gold nanoparticles of varying sizes can be realized by pulsed laser deposition. • High SERS intensity of dye from gold films of large SPR strength at excitation wavelength. - Abstract: Localized surface plasmon resonance (LSPR) characteristics of gold nanoparticles films grown at different substrate temperatures and mass thicknesses with and without alumina capping were studied. At different film mass thicknesses, the LSPR response was observed mainly in the films grown at high substrate temperatures. About 300 °C substrate temperature was found to be optimum for producing narrow and strong LSPR band in both uncapped and alumina capped gold nanoparticles films. The LSPR wavelength could be tuned in the range of 600–750 nm by changing either number of ablation pulses or decreasing target to substrate distance (TSD) and alumina layer capping. Though the alumina capping re-sputtered the gold films still these films exhibited stronger LSPR response compared to the uncapped films. Atomic force microscopic analysis revealed formation of densely packed nanoparticles films exhibiting strong LSPR response which is consistent with the package density of the nanoparticles predicted by the theoretical calculations. The average size of nanoparticles increased with substrate temperature, number of ablation pulses and decreasing the TSD. For the same mass thickness of gold films grown at different substrate temperatures the surface enhanced Raman scattering (SERS) intensity of Rhodamine6G dye was found to be significantly different which had direct correlation with the LSPR strength of the films at the excitation wavelength

  7. Dual optoelectronic visual detection and quantification of spectroscopically silent heavy metal toxins: A multi-measurand sensing strategy based on Rhodamine 6G as chromo or fluoro ionophore

    Energy Technology Data Exchange (ETDEWEB)

    Prathish, K.P.; James, D.; Jaisy, J. [Inorganic and Polymeric Materials Group, National Institute for Interdisciplinary Science and Technology (CSIR), Industrial Estate (P.O), Pappanamcode, Trivandrum 695019 (India); Prasada Rao, T., E-mail: rao@csrrltrd.ren.nic.in [Inorganic and Polymeric Materials Group, National Institute for Interdisciplinary Science and Technology (CSIR), Industrial Estate (P.O), Pappanamcode, Trivandrum 695019 (India)

    2009-08-04

    A novel colorimetric chemo-sensor for the simultaneous visual detection and quantification of spectroscopically silent heavy metal toxins viz. cadmium, lead and mercury has been developed. This is based on the proposed sequential ligand exchange (SLE) mechanism of iodide from Pb-I{sup -}-Rhodamine 6G ion associate with citrate (without affecting ion associates of Cd and Hg) and subsequently from Cd-I{sup -}-Rhodamine 6G ion associate with EDTA (without affecting Hg-I{sup -}-Rhodamine 6G). Multi-measurand detection and quantification by colorimetry is possible as the individual toxins gives identical bathochromic shifts in aqueous solution, i.e. from 530 to 575 nm on formation of ternary ion associates in singular, binary and ternary mixtures. The visual detection provides a simple, quick and sensitive detection method in addition to quantification via spectrophotometry with Sandell sensitivities of 1.1, 15 and 2.5 {mu}g dm{sup -2} for cadmium, lead and mercury, respectively. The developed procedure has been successfully tested for the analysis of environmental (cast alkali, lead acid battery and zinc manufacturing industry effluents) samples. Furthermore, the multi-measurand quantification of the above-mentioned heavy metal toxins based on fluorescence quenching and use of Pyronine G as chromo-ionophore instead of Rhodamine 6G is also described.

  8. Simple and fast fluorescence detection of benzoyl peroxide in wheat flour by N-methoxy rhodamine-6G spirolactam based on consecutive chemical reactions

    International Nuclear Information System (INIS)

    Chen Wei; Shi Wen; Li Zhao; Ma Huimin; Liu Yang; Zhang Jinghua; Liu Qingjun

    2011-01-01

    Graphical abstract: A simple and fast method for fluorescence detection of benzoyl peroxide in wheat flour by N-methoxy rhodamine-6G spirolactam (1) is proposed based on consecutive chemical reactions. Highlights: ► Benzoyl peroxide can oxidize Fe 2+ into Fe 3+ . ► Fe 3+ selectively induces the opening of rhodamine spirolactam ring. ► The two reactions led to the development of a new fluorescent method for benzoyl peroxide. ► The method is simple and fast, and is used to detect benzoyl peroxide in wheat flour. - Abstract: Benzoyl peroxide (BPO) as a brightener is often added to wheat flour, and excessive use of this food additive is receiving increasing concern. Herein, a simple and fast method for fluorescence detection of BPO is proposed based on consecutive chemical reactions. In this approach, BPO first oxidizes Fe 2+ into Fe 3+ and the resulting Fe 3+ then induces the opening of the spirolactam ring of a new rhodamine derivative, N-methoxy rhodamine-6G spirolactam, switching on fluorescence of the detection system. More importantly, the fluorescence response of the reaction system to BPO is rather rapid and sensitive, with a detection limit of 6 mg kg −1 (k = 3), which makes it to be of great potential use in food safety analysis. The applicability of the proposed method has been successfully demonstrated on the determination of BPO in wheat flour samples.

  9. Plasmon enhancement of Raman scattering and fluorescence for rhodamine 6G molecules in the porous glass and PVA films with nanoparticles of silver citrate hydrosol

    International Nuclear Information System (INIS)

    Konstantinova, E I; Zyubin, A U; Samusev, I G; Slezhkin, V A; Bryukhanov, V V

    2016-01-01

    The study of Raman and fluorescence spectra for Rhodamine 6G molecules in a film of polyvinyl alcohol on the modified by silver nanoparticles (NPs) porous glass and without the porous glass has been done. The gain of the scattering intensity and fluorescence emission has been obtained in the presence of silver nanoparticles. The gain order was obtained as ∼ 10"1"1 (paper)

  10. Förster resonance energy transfer between α -Bi2O3 nanorods and rhodamine 6G in aqueous media for turn-off glucose-sensing application

    International Nuclear Information System (INIS)

    Balasubramanian, Karthikeyan

    2017-01-01

    The present study deals with the Förster resonance energy transfer (FRET) process that takes place between α -Bi 2 O 3 nanorods (BN), which function as a donor, and Rhodamine 6G, which (R6G) functions as an acceptor. Fluorescence of R6G situated at 550 nm is found to be enhanced due to this FRET behaviour with the increase of the number of acceptor concentrations. Apart from this, the rate of energy transfer also increased. It is found that a decrease in the donor–acceptor distance is assisting FRET. Steady state and time-resolved fluorescence studies are performed in two different excitation wavelengths and it shows the possible FRET behaviour where the average lifetime increases with the increase of acceptor concentrations. This pair is used to sense the glucose in nM concentrations. (paper)

  11. Pyrazolone as a recognition site: Rhodamine 6G-based fluorescent probe for the selective recognition of Fe3+ in acetonitrile-aqueous solution.

    Science.gov (United States)

    Parihar, Sanjay; Boricha, Vinod P; Jadeja, R N

    2015-03-01

    Two novel Rhodamine-pyrazolone-based colorimetric off-on fluorescent chemosensors for Fe(3+) ions were designed and synthesized using pyrazolone as the recognition moiety and Rhodamine 6G as the signalling moiety. The photophysical properties and Fe(3+) -binding properties of sensors L(1) and L(2) in acetonitrile-aqueous solution were also investigated. Both sensors successfully exhibit a remarkably 'turn-on' response, toward Fe(3+) , which was attributed to 1: 2 complex formation between Fe(3+) and L(1) /L(2) . The fluorescent and colorimetric response to Fe(3+) can be detected by the naked eye, which provides a facile method for the visual detection of Fe(3+) . Copyright © 2014 John Wiley & Sons, Ltd.

  12. Inorganic-organic Ag-rhodamine 6G hybrid nanorods: "turn on" fluorescent sensors for highly selective detection of Pb2+ ions in aqueous solution.

    Science.gov (United States)

    Tyagi, A K; Ramkumar, Jayshree; Jayakumar, O D

    2012-02-07

    Lead metal ions are of great concern and the monitoring of their concentration in the environment has become extremely important. In the present study, a new inorganic-organic hybrid assay of Ag nanorods (AgNR)-Rhodamine 6G (R6G) was developed for the sensitive and selective determination of Pb(2+) ions in aqueous solutions. To the best of our knowledge there is almost no literature on the use of silver nanorod sensors for determination of lead ions in aqueous solutions. The sensor is developed by the coating of R6G on the surface of AgNRs. The sensing is based on the photoluminescence of R6G. The sensor was rapid as the measurements were carried out within 3 min of addition of the test solution to the AgNR-R6G hybrid. Moreover, the system showed excellent stability at tested concentration levels of Pb(2+) ions. The naked eye detection of the colour was possible with 1 mg L(-1) of Pb(2+) ions. The present method has a detection limit of 50 μg L(-1) of Pb(2+) (for a signal/noise (S/N) ratio > 3). The selectivity toward Pb(2+) ions against other metal ions was improved using chelating agents. The proposed method was validated by analysis using different techniques.

  13. A sensitive and selective fluorescence assay for metallothioneins by exploiting the surface energy transfer between rhodamine 6G and gold nanoparticles

    International Nuclear Information System (INIS)

    Yan, Yu-Qian; Tang, Xian; Wang, Yong-Sheng; Li, Ming-Hui; Cao, Jin-Xiu; Chen, Si-Han; Zhu, Yu-Feng; Wang, Xiao-Feng; Huang, Yan-Qin

    2015-01-01

    We report on a sensitive and selective strategy for the determination of metallothioneins (MTs). The assay is based on the suppression of the surface energy transfer that occurs between rhodamine 6G (Rh6G) and gold nanoparticles (AuNPs). If Rh6G is adsorbed onto the surface of AuNPs in water solution of pH 3.0, its fluorescence is quenched due to surface energy transfer. However, on addition of MTs to the Rh6G-AuNPs system, fluorescence is recovered owing to the formation of the MTs-AuNPs complex and the release of Rh6G into the solution. Under optimized conditions, the increase in fluorescence intensity is directly proportional to the concentration of the MTs in the range from 9.68 to 500 ng mL −1 , with a detection limit as low as 2.9 ng mL −1 . The possible mechanism of this assay is discussed. The method was successfully applied to the determination of MTs in (spiked) human urine. (author)

  14. A highly selective and sensitive photoswitchable fluorescent probe for Hg2+ based on bisthienylethene-rhodamine 6G dyad and for live cells imaging.

    Science.gov (United States)

    Xu, Li; Wang, Sheng; Lv, Yingnian; Son, Young-A; Cao, Derong

    2014-07-15

    A new photochromic diarylethene derivative bearing rhodamine 6G dimmer as a fluorescent molecular probe is designed and synthesized successfully. All the compounds are characterized by nuclear magnetic resonance and mass spectrometry. The bisthienylethene-rhodamine 6G dyad exhibit excellent phtochromism with reversibly color and fluorescence changes alternating irradiation with ultraviolet and visible light. Upon addition of Hg(2+), its color changes from colorless to red and its fluorescence is remarkably enhanced. Whereas other ions including K(+), Na(+), Ca(2+), Mg(2+), Fe(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Mn(2+), Pb(2+), Ni(2+), Fe(3+), Al(3+), Cr(3+) and so on induce basically no spectral changes, which constitute a highly selective and sensitive photoswitchable fluorescent probe toward Hg(2+). Furthermore, by means of laser confocal scanning microscopy experiments, it is demonstrated that this probe can be applied for live cell imaging and monitoring Hg(2+) in living lung cancer cells with satisfying results, which shows its value of potential application in environmental and biological systems. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Highly Selective and Ultrasensitive Turn-On Luminescence Chemosensor for Mercury (II) Determination Based on the Rhodamine 6G Derivative FC1 and Au Nanoparticles

    Science.gov (United States)

    Brasca, Romina; Onaindia, María C.; Goicoechea, Héctor C.; Muñoz de la Peña, Arsenio; Culzoni, María J.

    2016-01-01

    A method for the detection and quantitation of Hg2+ in aqueous samples by fluorescence spectroscopy is presented. It consists of a turn-on sensor developed by coupling Gold nanoparticles (AuNPs) with the rhodamine 6G derivative FC1, in which the response is generated by a mercury-induced ring-opening reaction. The AuNPs were included in order to improve the sensitivity of the method towards the analyte, maintaining its high selectivity. The method was validated in terms of linearity, precision and accuracy, and applied to the quantitation of Hg2+ in Milli-Q and tap water with and without spiked analyte. The limit of detection and quantitation were 0.15 μg·L−1 and 0.43 μg·L−1, respectively, constituting a substantial improvement of sensitivity in comparison with the previously reported detection of Hg2+ with free FC1. PMID:27782059

  16. Staining diatoms with rhodamine dyes: control of emission colour in photonic biocomposites

    Science.gov (United States)

    Kucki, Melanie; Fuhrmann-Lieker, Thomas

    2012-01-01

    The incorporation of rhodamine dyes in the cell wall of diatoms Coscinodiscus granii and Coscinodiscus wailesii for the production of luminescent hybrid nanostructures is investigated. By systematic variation of the substitution pattern of the rhodamine core, we found that carbonic acids are considerably better suited than esters because of their physiological compatibility. The amino substitution pattern that controls the optical properties of the chromophore has no critical influence on dye uptake and incorporation, thus a variety of biocomposites with different emission maxima can be prepared. Applications in biomineralization studies as well as in materials science are envisioned. PMID:21865248

  17. Teaching Old Dyes New Tricks: Biological Probes Built from Fluoresceins and Rhodamines.

    Science.gov (United States)

    Lavis, Luke D

    2017-06-20

    Small-molecule fluorophores, such as fluorescein and rhodamine derivatives, are critical tools in modern biochemical and biological research. The field of chemical dyes is old; colored molecules were first discovered in the 1800s, and the fluorescein and rhodamine scaffolds have been known for over a century. Nevertheless, there has been a renaissance in using these dyes to create tools for biochemistry and biology. The application of modern chemistry, biochemistry, molecular genetics, and optical physics to these old structures enables and drives the development of novel, sophisticated fluorescent dyes. This critical review focuses on an important example of chemical biology-the melding of old and new chemical knowledge-leading to useful molecules for advanced biochemical and biological experiments.

  18. Computational design and fabrication of core-shell magnetic molecularly imprinted polymer for dispersive micro-solid-phase extraction coupled with high-performance liquid chromatography for the determination of rhodamine 6G.

    Science.gov (United States)

    Xie, Jin; Xie, Jie; Deng, Jian; Fang, Xiangfang; Zhao, Haiqing; Qian, Duo; Wang, Hongjuan

    2016-06-01

    A novel core-shell magnetic nano-adsorbent with surface molecularly imprinted polymer coating was fabricated and then applied to dispersive micro-solid-phase extraction followed by determination of rhodamine 6G using high-performance liquid chromatography. The molecularly imprinted polymer coating was prepared by copolymerization of dopamine and m-aminophenylboronic acid (functional monomers), in the presence of rhodamine 6G (template). The selection of the suitable functional monomers was based on the interaction between different monomers and the template using the density functional theory. The ratios of the monomers to template were further optimized by an OA9 (3(4) ) orthogonal array design. The binding performances of the adsorbent were evaluated by static, kinetic, and selective adsorption experiments. The results reveal that the adsorbent possesses remarkable affinity and binding specificity for rhodamine 6G because of the enhanced Lewis acid-base interaction between the B(Ш) embedded in the imprinted cavities and the template. The nano-adsorbent was successfully applied to dispersive micro-solid-phase extraction coupled to high-performance liquid chromatography for the trace determination of rhodamine 6G in samples with a detection limit of 2.7 nmol/L. Spiked recoveries ranged from 93.0-99.1, 89.5-92.7, and 86.9-105% in river water, matrimony vine and paprika samples, respectively, with relative standard deviations of less than 4.3%. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Nanobeads of zinc oxide with rhodamine B dye as a sensitizer for dye sensitized solar cell application

    Energy Technology Data Exchange (ETDEWEB)

    Baviskar, P.K. [Thin Film and Nano Science Laboratory, Department of Physics, School of Physical Sciences, North Maharashtra University, Jalgaon 425 001, MS (India); Zhang, J.B. [Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (China); Gupta, V.; Chand, S. [Organic and Hybrid Solar Cell, Physics of Energy Harvesting Division, Dr. K. S. Krishnan Marg, National Physical Laboratory, New Delhi 110012 (India); Sankapal, B.R., E-mail: brsankapal@rediffmail.com [Thin Film and Nano Science Laboratory, Department of Physics, School of Physical Sciences, North Maharashtra University, Jalgaon 425 001, MS (India)

    2012-01-05

    Highlights: > Synthesis of ZnO film was done at room temperature (27 deg. C). > Simple and inexpensive chemical bath deposition method was employed. > The as deposited film consists of mixed phases of hydroxide and oxide. > The post annealing was done at 200 deg. C in order to remove hydroxide phase. > Low-cost, metal free Rhodamine B dye was used for DSSC application. - Abstract: Cost effective, ruthenium metal free rhodamine B dye has been chemically adsorbed on ZnO films consisting of nanobeads to serve as a photo anode in dye sensitized solar cells. These ZnO films were chemically synthesized at room temperature (27 deg. C) on to fluorine doped tin oxide (FTO) coated glass substrates followed by annealing at 200 deg. C. These films consisting of inter connected nanobeads (20-40 nm) which are due to the agglomeration of very small size particles (3-5 nm) leading to high surface area. The film shows wurtzite structure having high crystallinity with optical direct band gap of 3.3 eV. Optical absorbance measurements for rhodamine B dye covered ZnO film revealed the good coverage in the visible region (460-590 nm) of the solar spectrum. With poly-iodide liquid as an electrolyte, device exhibits photon to electric energy conversion efficiency ({eta}) of 1.26% under AM 1.5G illumination at 100 mW/cm{sup 2}.

  20. Tests of rhodamine WT dye for toxicity to oysters and fish

    Science.gov (United States)

    Parker, Garald G.

    1973-01-01

    Because of the toxicity to oyster larvae and eggs of rhodamine B dye in concentrations greater than 1 mg/l in earlier tests, there was a concern that rhodamine WT, a similar tracer dye, would have a detrimental effect on marine life being developed under the aquaculture program of the Lummi Indian Tribe near Bellingham, Wash. Tests showed that 48-hour exposures at 24° C of 11,000 oyster eggs per liter and 6,000 12-day-old larvae per liter, in sea water with concentrations of rhodamine WT ranging from 1 μg/l to 10 mg/l, resulted in development of the eggs to normal straight-hinge larvae and no abnormalities in the larvae development. Tests made on the smolt of both silver salmon and Donaldson trout, with the fish held for 17.5 hours in a tankfull of sea water with a dye concentration of 10 mg/l at 22°C showed no mortalities or respiratory problems. With the concentration increased to 375 mg/l, and the time extended an additional 3.2 hours, still no mortalities or abnormalities were noted. The fish remained healthy in dye-free water when last checked a month after the test.

  1. Rhodamine dyes as potential agents for photochemotherapy of cancer in human bladder carcinoma cells

    International Nuclear Information System (INIS)

    Shea, C.R.; Chen, N.; Wimberly, J.; Hasan, T.

    1989-01-01

    The phototoxicity in vitro of rhodamine 123 and tetrabromo rhodamine 123 (TBR) was compared, in order to assess their photochemotherapeutic potential. Exposure to 514.5-nm radiation from an argon ion laser caused phototoxicity in MGH-U1 bladder carcinoma cells previously treated with either dye at 10 microM for 30 min. As assessed by colony formation and cellular morphology, TBR was markedly more phototoxic than rhodamine 123, reflecting increased intersystem crossing of TBR to the triplet manifold via spin-orbital coupling induced by the heavy bromine atoms. Photoreactions of TBR very efficiently generated singlet oxygen ( 1 O 2 ) in solution; furthermore, irradiation of TBR-treated cells was significantly more toxic when performed in the presence of deuterium oxide, an enhancer of damage caused by 1 O 2 . Retention of fluorescence in TBR-treated cells was enhanced by irradiation, indicating that a stable photoproduct may be formed in reaction with cellular components

  2. Adsorption of Rhodamine B Dye from Aqueous Solution on Irvingia ...

    African Journals Online (AJOL)

    NICOLAAS

    second-order kinetics model was observed to fit the adsorption data. Solution pH ... usually released containing various dyes and heavy metals.1 In particular ...... B.S. Ogunsina, O.A. Koya and O.O. Adeosun, Deformation and frac- ture of dika ...

  3. A highly sensitive fluorescence quenching method for perphenazine detection based on its catalysis of K{sub 2}S{sub 2}O{sub 8} oxidizing rhodamine 6G

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Lihong; Huang, Qitong; Lin, Changqing [Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou, 363000 (China); Lin, Xiaofeng [College of Chemistry and Environment, Minnan Normal University, Zhangzhou, 363000 (China); Huang, Yiqun [Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000 (China); Liu, Jiaming, E-mail: mnsdljm@163.com [College of Chemistry and Environment, Minnan Normal University, Zhangzhou, 363000 (China); Ma, Xudong, E-mail: maxudong005@hotmail.com [Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000 (China)

    2014-12-15

    In this paper, the fluorescence spectra of Rhod 6G (rhodamine 6G)–K{sub 2}S{sub 2}O{sub 8}–PPH (perphenazine) were studied. We found that Rhod 6G existed in the form of Rhod 6G{sup +} under the conditions of 60 °C, 10 min and pH 5.42, and Rhod 6G{sup +} can emit strong and stable fluorescence. Further study showed that when PPH and Rhod 6G{sup +} coexisted, the ester exchange reaction carried out between -OH of PPH and -COOC{sub 2}H{sub 5} of Rhod 6G{sup +} to produced Rhod 6G{sup +}–PPH compound. More interestingly, K{sub 2}S{sub 2}O{sub 8} could oxidize Rhod 6G{sup +} and quench its RTP signal, while PPH was oxidized to red compound PPH′ by K{sub 2}S{sub 2}O{sub 8}, and Rhod 6G{sup +}–PPH′ and PPH were produced in the ester exchange reaction between the -OH of PPH′ and the -COOC{sub 2}H{sub 5} of Rhod 6G{sup +}–PPH. In the above process, PPH catalyzed K{sub 2}S{sub 2}O{sub 8} oxidizing Rhod 6G, which caused the fluorescence signal of the system to quench sharply. Hence, a catalytic fluorescence quenching method for the determination of residual PPH has been developed based on the its catalyzing K{sub 2}S{sub 2}O{sub 8} oxidize rhodamine 6G. This sensitive, accurate, simple and selective fluorescence quenching method was used to determine residual PPH in biological samples with the results consisting with those obtained by high performance liquid chromatography (HPLC), showing good accuracy. The structures of Rhod 6G{sup +}, PPH and Rhod 6G{sup +}–PPH were characterized by infrared spectra. The reaction mechanism of the determination of PPH was also discussed. - Highlights: • Fluorescence for the determination of perphenazine (PPH) had been established. • This method had high sensitivity (limit of detection was 3.3×10{sup −14} g mL{sup −1}). • This method had been applied to determination of PPH in biological samples. • Structures of Rhod 6G{sup +}, PPH and Rhod 6G{sup +}–PPH were characterized by infrared spectra. • Mechanism

  4. Multi step FRET among three laser dyes Pyrene, Acriflavine and Rhodamine B

    International Nuclear Information System (INIS)

    Saha, Jaba; Dey, Dibyendu; Roy, Arpan Datta; Bhattacharjee, D.; Hussain, Syed Arshad

    2016-01-01

    Fluorescence Resonance Energy Transfer (FRET) system using three dyes has been demonstrated. It has been observed that multi step energy transfer occurred from Pyrene to Rhodamine B via Acriflavine. Here Acriflavine acts as an antenna to receive energy from Pyrene and transfer the same to Rhodamine B. This multi step FRET system is advantageous compared to the conventional FRET as this can be used to study molecular level interaction beyond conventional FRET distance (1–10 nm) as well as studying multi-branched macromolecules. The introduction of clay enhances the FRET efficiencies among the dye pair, which is an advantage to make the multi step system more useful. Similar approach can be used for increasing FRET efficiencies by using other dyes. - Highlights: • Multi-step FRET occurred from Pyrene (Py) to Rhodamine B (RhB) via Acriflavine (Acf). • Acf acts as an antenna to receive energy from Py and to transfer energy to RhB. • Multi-step FRET can be used to study molecular level interaction beyond 1–10 nm. • Incorporation of nanoclay laponite enhances the energy transfer efficiency.

  5. Molecular dynamics simulation of the effect of pH on the adsorption of rhodamine laser dyes on TiO2 hydroxylated surfaces

    OpenAIRE

    2009-01-01

    Abstract We have carried out a study of the adsorption, on the (101) surface of anatase TiO2, of two industrially relevant rhodamines molecules (rhodamine 6G and rhodamine 800) employing Molecular Dynamics. These theoretical studies have shown that Rhodamine 6G must adsorb on surfaces under basic conditions. Moreover, the adsorption of this molecule shows a strong dependence upon the pH of the system, i.e. under neutral conditions the adsorption energy is quite smaller, and under ...

  6. Photocatalytic degradation of Rhodamine B dye using Fe doped TiO2 nanocomposites

    Science.gov (United States)

    Barkhade, Tejal; Banerjee, Indrani

    2018-05-01

    The unique properties of titanium dioxide (TiO2) such as high photo catalytic activity, high chemical stability and low toxicity have made it a suitable photocatalyst in recent decades. The effect of modification of TiO2 with doping of iron on its characteristics and photo catalytic efficiency was studied. The change in band gap energy of TiO2 nanoparticles after doping with Fe has been studied. Significant enhancement in photo catalytic property of TiO2 after Fe doping under light exposure conditions has been investigated. Acute exposure to non-biodegradable Rhodamine B resulted in many health problems like burning of eyes, skin irritation, nasal burning, and chest pain etc. Therefore, degradation of this dye is needed to save environment and animals. Considering the similar radius of Fe3+ and Ti4+ ions (respectively 0.64 Å and 0.68 Å), titanium position in the lattice of TiO2 can be replaced by iron cations easily. The undoped and Fe doped TiO2 nano composites were synthesized by sol-gel method, in which 1.0M% of Fe was doped with TiO2 and then the samples were characterized by using FE-SEM, UV-Visible diffuse spectroscopy, Raman Spectroscopy, and FTIR. Photo catalytic degradation of Rhodamine B dye experiment was carried out in visible light range. After 90 min time duration pink colour of dye turned colourless, indicating significant degradation rate with time.

  7. Efficient removal of rhodamine 6G dye from aqueous solution using nickel sulphide incorporated polyacrylamide grafted gum karaya bionanocomposite hydrogel

    CSIR Research Space (South Africa)

    Kumar, N

    2016-02-01

    Full Text Available polymer of the Gk with the PAAm was synthesized using the graft co-polymerization technique. In the second step, the nickel sulphide nanoparticles were incorporated in situ within the hydrogel polymer matrix. The synthesized hydrogel nanocomposite...

  8. Efficient Photocatalytic Degradation of Rhodamine B Dye by Aligned Arrays of Self-Assembled Hydrogen Titanate Nanotubes

    Directory of Open Access Journals (Sweden)

    Sriparna Chatterjee

    2014-01-01

    Full Text Available We show that an aligned array of hydrothermally grown, multiwalled hydrogen titanate (H2Ti3O7 nanotubes—anchored to both faces of a metallic Ti foil—acts as an efficient photocatalyst. We studied the degradation of rhodamine B dye in the presence of the nanostructured photocatalyst under UV irradiation, by monitoring the optical absorption of the dye. Rhodamine B was chosen as a representative—and particularly harmful—industrial pollutant dye. The inner and outer diameters of the H2Ti3O7 nanotubes were 5 nm and 10 nm, respectively. The nanotube array catalyst is recyclable and structurally stable. Most importantly, it shows comparable or higher photodecomposition rate constant than those of both H2Ti3O7 nanotube powder and P-25 (Degussa. The enhanced photocatalytic performance may be ascribed to the nanotube array having a superhydrophilic surface with a high accessible surface area.

  9. Long-lived and largely red-shifted photoluminescence of solid-state rhodamine dyes: Molecular exciton coupling and structural effect

    International Nuclear Information System (INIS)

    Zhang, Xian-Fu; Zhang, Ya-Kui

    2015-01-01

    The optical absorption and fluorescence properties of five rhodamine dyes in solid-state are measured and show large difference from that in their gas phase or liquid solvents. All solid-state rhodamine dyes strongly absorb all light in UV and visible region, but emit only red and NIR fluorescence (680–800 nm, >100 nm red-shifted from that in solution). Further more, the absorption maxima of a solid-state rhodamine show a large red-shifted band (~100 nm) and blue-shifted peak (~125 nm) compared to that in solutions, indicating a strong molecular exciton coupling between molecules. All solid-state rhodamines still show reasonably good fluorescence quantum yield (Φ f ). In particular, solid-state Rhodamine B butyl ester and sulfonyl Rhodamine B showed a much longer emission lifetime (τ f ) than that of the corresponding molecular rhodamine, i.e. 4.12 and 4.14 ns in solid state compared to 1.61 and 2.47 ns in solution. The chemical structure of a rhodamine molecule showed dramatic effect on Φ f and τ f values for solid state rhodamine. The larger substituent in the benzene moiety favors higher Φ f and τ f values of rhodamine solids. These effects can be elucidated by the relation between structure-molecular distance and molecular exciton couplings. - Highlights: • Optical properties of solid rhodamines show large difference from that in solutions. • Solid-state rhodamine dyes emit red and NIR fluorescence (680–800 nm). • Solid-state rhodamines still show reasonably good fluorescence quantum yield. • Solid-state rhodamines have much longer fluorescence lifetimes than that in solutions

  10. Long-lived and largely red-shifted photoluminescence of solid-state rhodamine dyes: Molecular exciton coupling and structural effect

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xian-Fu, E-mail: zhangxianfu@tsinghua.org.cn [Institute of Applied Photochemistry & Center of Analysis and Measurements, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei Province (China); MPC Technologies, Hamilton, ON, Canada L8S 3H4 (Canada); Zhang, Ya-Kui [Institute of Applied Photochemistry & Center of Analysis and Measurements, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei Province (China)

    2015-10-15

    The optical absorption and fluorescence properties of five rhodamine dyes in solid-state are measured and show large difference from that in their gas phase or liquid solvents. All solid-state rhodamine dyes strongly absorb all light in UV and visible region, but emit only red and NIR fluorescence (680–800 nm, >100 nm red-shifted from that in solution). Further more, the absorption maxima of a solid-state rhodamine show a large red-shifted band (~100 nm) and blue-shifted peak (~125 nm) compared to that in solutions, indicating a strong molecular exciton coupling between molecules. All solid-state rhodamines still show reasonably good fluorescence quantum yield (Φ{sub f}). In particular, solid-state Rhodamine B butyl ester and sulfonyl Rhodamine B showed a much longer emission lifetime (τ{sub f}) than that of the corresponding molecular rhodamine, i.e. 4.12 and 4.14 ns in solid state compared to 1.61 and 2.47 ns in solution. The chemical structure of a rhodamine molecule showed dramatic effect on Φ{sub f} and τ{sub f} values for solid state rhodamine. The larger substituent in the benzene moiety favors higher Φ{sub f} and τ{sub f} values of rhodamine solids. These effects can be elucidated by the relation between structure-molecular distance and molecular exciton couplings. - Highlights: • Optical properties of solid rhodamines show large difference from that in solutions. • Solid-state rhodamine dyes emit red and NIR fluorescence (680–800 nm). • Solid-state rhodamines still show reasonably good fluorescence quantum yield. • Solid-state rhodamines have much longer fluorescence lifetimes than that in solutions.

  11. An enhanced cerium(IV)-rhodamine 6G chemiluminescence system using guest-host interactions in a lab-on-a-chip platform for estimating the total phenolic content in food samples.

    Science.gov (United States)

    Al Haddabi, Buthaina; Al Lawati, Haider A J; Suliman, FakhrEldin O

    2016-04-01

    Two chemiluminescence-microfluidic (CL-MF) systems, e.g., Ce(IV)-rhodamine B (RB) and Ce(IV)-rhodamine 6G (R6G), for the determination of the total phenolic content in teas and some sweeteners were evaluated. The results indicated that the Ce(IV)-R6G system was more sensitive in comparison to the Ce(IV)-RB CL system. Therefore, a simple (CL-MF) method based on the CL of Ce(IV)-R6G was developed, and the sensitivity, selectivity and stability of this system were evaluated. Selected phenolic compounds (PCs), such as quercetin (QRC), catechin (CAT), rutin (RUT), gallic acid (GA), caffeic acid (CA) and syringic acid (SA), produced analytically useful chemiluminescence signals with low detection limits ranging from 0.35 nmol L(-1) for QRC to 11.31 nmol L(-1) for SA. The mixing sequence and the chip design were crucial, as the sensitivity and reproducibility could be substantially affected by these two factors. In addition, the anionic surfactant (i.e., sodium dodecyl sulfate (SDS)) can significantly enhance the CL signal intensity by as much as 300% for the QRC solution. Spectroscopic studies indicated that the enhancement was due to a strong guest-host interaction between the cationic R6G molecules and the anionic amphiphilic environment. Other parameters that could affect the CL intensities of the PCs were carefully optimized. Finally, the method was successfully applied to tea and sweetener samples. Six different tea samples exhibited total phenolic/antioxidant levels from 7.32 to 13.5 g per 100g of sample with respect to GA. Four different sweetener samples were also analyzed and exhibited total phenolic/antioxidant levels from 500.9 to 3422.9 mg kg(-1) with respect to GA. The method was selective, rapid and sensitive when used to estimate the total phenolic/antioxidant level, and the results were in good agreement with those reported for honey and tea samples. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Xanthium strumarium L. seed hull as a zero cost alternative for Rhodamine B dye removal.

    Science.gov (United States)

    Khamparia, Shraddha; Jaspal, Dipika Kaur

    2017-07-15

    Treatment of polluted water has been considered as one of the most important aspects in environmental sciences. Present study explores the decolorization potential of a low cost natural adsorbent Xanthium strumarium L. seed hull for the adsorption of a toxic xanthene dye, Rhodamine B (RHB). The characterization of the adsorbent revealed the presence of high amount of carbon, when exposed to Electron Dispersive Spectroscopy (EDS). Further appreciable decolorization took place which was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) analysis noticing shift in peaks. Isothermal studies indicated multilayer adsorption following Freundlich isotherm. The rate of adsorption was supported by second order kinetics directing a chemical phenomenon during the process with dominance of film diffusion as the rate governing step. Moreover paper aims at correlating the chemical arena to the mathematical aspect providing an in-depth information of the studied treatment process. For proper assessment and validation of the observed data, experimental data has been statistically treated by applying different error functions namely, Chi-square test (χ 2 ), Sum of absolute errors (EABS) and Normalized standard deviation (NSD). Further practical applicability of the low cost adsorbent was evaluated by continuous column mode studies with 72.2% of dye recovery. Xanthium strumarium L. proved to be environment friendly low cost natural adsorbent for decolorizing RHB from aquatic system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Animal Bone Supported SnO2 as Recyclable Photocatalyst for Degradation of Rhodamine B Dye.

    Science.gov (United States)

    Wu, Yun; Wang, Hui; Cao, Mengdie; Zhang, Yichi; Cao, Feifei; Zheng, Xinsheng; Hu, Jinfei; Dong, Jiangshan; Xiao, Zhidong

    2015-09-01

    SnO2 nanoparticles supported on an animal bone which serves as inexpensive and environment-friendly natural products were developed by a facile hydrothermal approach. As a promising photocatalyst, the novel SnO2/porcine bone material exhibited high photocatalytic activity towards the degradation of rhodamine B (RhB) dye under UV-Vis irradiation. About 97.3% of RhB can be effectively decomposed by the catalysis with the SnO2/porcine bone in 90 min, while only 51.5% of RhB can be degraded by pure SnO2 nanoparticles. Moreover, the photocatalytic activity was incremental with the increase of cycle times in previous five cycles. It is mainly because the photocatalyst which has been used for several times possesses a stronger ability of light absorption and utilization compared to the fresh catalyst according to the results of the characterization and relative experiments. It is noteworthy that the animal bone support can improve the activity for the photocatalyst, which would provide further impetus to alternate synthesis strategies for photocatalysts and make the photocatalysis process faster, less expensive, and more environmentally friendly.

  14. Photoluminescence Spectroscopy of Rhodamine 800 Aqueous Solution and Dye-Doped Polymer Thin-Film: Concentration and Solvent Effects

    Science.gov (United States)

    Le, Khai Q.; Dang, Ngo Hai

    2018-05-01

    This paper investigates solvent and concentration effects on photoluminescence (PL) or fluorescence properties of Rhodamine 800 (Rho800) dyes formed in aqueous solution and polymer thin-film. Various commonly used organic solvents including ethanol, methanol and cyclopentanol were studied at a constant dye concentration. There were small changes in the PL spectra for the different solvents in terms of PL intensity and peak wavelength. The highest PL intensity was observed for cyclopentanol and the lowest for ethanol. The longest peak wavelength was found in cyclopentanol (716 nm) and the shortest in methanol (708 nm). Dissolving the dye powder in the methanol solvent and varying the dye concentration in aqueous solution from the high concentrated solution to highly dilute states, the wavelength tunability was observed between about 700 nm in the dilute state and 730 nm at high concentration. Such a large shift may be attributed to the formation of dye aggregates. Rho800 dye-doped polyvinyl alcohol (PVA) polymer thin-film was further investigated. The PL intensity of the dye in the form of thin-film is lower than that of the aqueous solution form whereas the peak wavelength is redshifted due to the presence of PVA. This paper, to our best knowledge, reports the first study of spectroscopic properties of Rho800 dyes in various forms and provides useful guidelines for production of controllable organic luminescence sources.

  15. Organic cation rhodamines for screening organic cation transporters in early stages of drug development.

    Science.gov (United States)

    Ugwu, Malachy C; Oli, Angus; Esimone, Charles O; Agu, Remigius U

    The aim of this study was to investigate the suitability of rhodamine-123, rhodamine-6G and rhodamine B as non-radioactive probes for characterizing organic cation transporters in respiratory cells. Fluorescent characteristics of the compounds were validated under standard in vitro drug transport conditions (buffers, pH, and light). Uptake/transport kinetics and intracellular accumulation of the compounds were investigated. Uptake/transport mechanisms were investigated by comparing the effect of pH, temperature, concentration, polarity, OCTs/OCTNs inhibitors/substrates, and metabolic inhibitors on the cationic dyes uptake in Calu-3 cells. Fluorescence stability and intensity of the compounds were altered by buffer composition, light, and pH. Uptake of the dyes was concentration-, temperature- and pH-dependent. OCTs/OCTNs inhibitors significantly reduced intracellular accumulation of the compounds. Whereas rhodamine-B uptake was sodium-dependent, pH had no effect on rhodamine-123 and rhodamine-6G uptake. Transport of the dyes across the cells was polarized: (AP→BL>BL→AP transport) and saturable: {V max =14.08±2.074, K m =1821±380.4 (rhodamine-B); V max =6.555±0.4106, K m =1353±130.4 (rhodamine-123) and V max =0.3056±0.01402, K m =702.9±60.97 (rhodamine-6G)}. The dyes were co-localized with MitoTracker®, the mitochondrial marker. Cationic rhodamines, especially rhodamine-B and rhodamine- 6G can be used as organic cation transporter substrates in respiratory cells. During such studies, buffer selection, pH and light exposure should be taken into consideration. Copyright © 2016 Elsevier Inc. All rights reserved.

  16. Synthesis and characterization of macromolecular rhodamine tethers and their interactions with P-glycoprotein.

    Science.gov (United States)

    Crawford, Lindsey; Putnam, David

    2014-08-20

    Rhodamine dyes are well-known P-glycoprotein (P-gp) substrates that have played an important role in the detection of inhibitors and other substrates of P-gp, as well as in the understanding of P-gp function. Macromolecular conjugates of rhodamines could prove useful as tethers for further probing of P-gp structure and function. Two macromolecular derivatives of rhodamine, methoxypolyethylene glycol-rhodamine6G and methoxypolyethylene glycol-rhodamine123, were synthesized through the 2'-position of rhodamine6G and rhodamine123, thoroughly characterized, and then evaluated by inhibition with verapamil for their ability to interact with P-gp and to act as efflux substrates. To put the results into context, the P-gp interactions of the new conjugates were compared to the commercially available methoxypolyethylene glycol-rhodamineB. FACS analysis confirmed that macromolecular tethers of rhodamine6G, rhodamine123, and rhodamineB were accumulated in P-gp expressing cells 5.2 ± 0.3%, 26.2 ± 4%, and 64.2 ± 6%, respectively, compared to a sensitive cell line that does not overexpress P-gp. Along with confocal imaging, the efflux analysis confirmed that the macromolecular rhodamine tethers remain P-gp substrates. These results open potential avenues for new ways to probe the function of P-gp both in vitro and in vivo.

  17. Penetration pattern of rhodamine dyes into enamel and dentin: confocal laser microscopy observation.

    Science.gov (United States)

    Kwon, S R; Wertz, P W; Li, Y; Chan, D C N

    2012-02-01

    Enamel and dentin are susceptible to extrinsic and intrinsic stains. The purposes of this study were to determine the penetration pattern of Rhodamine B and dextran-conjugated Rhodamine B into the enamel and dentin as observed by confocal laser microscopy and to relate it to the penetration pattern of hydrogen peroxide commonly used as an active ingredient in tooth-whitening agents and high-molecular-weight staining molecules. Eighteen recently extracted human maxillary anterior teeth were used. Teeth were cleaned and painted with nail varnish except for the crown area above the cemento-enamel junction (CEJ). The painted teeth were then immersed in Rhodamine B and dextran-conjugated Rhodamine B (70 000 MW) for 4, 7, 10 and 15 days. Teeth were sliced to 3 mm thickness in transverse plane and mounted on a glass slide just prior to observation with confocal laser microscopy. Rhodamine B and dextran-conjugated Rhodamine B readily penetrated into the enamel and dentin when exposed for 4 and 7 days, respectively. Rhodamine B penetrated along the interprismatic spaces of the enamel into the dentin. The penetration was accentuated in sections with existing crack lines in the enamel. Rhodamine B was readily absorbed into the dentinal tubules at the dentino-enamel junction and continued to penetrate through the dentin via the dentinal tubules into the pre-dentin. Within the limitations of this study, it is concluded that Rhodamine B and dextran-conjugated Rhodamine B when applied to the external surface of the tooth readily penetrate into the enamel and dentin via the interprismatic spaces in the enamel and dentinal tubules in the dentin, suggesting that stain molecules and bleaching agents possibly exhibit similar penetration pathways. © 2011 The Authors. ICS © 2011 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

  18. Synthesis of metal free ultrathin graphitic carbon nitride sheet for photocatalytic dye degradation of Rhodamine B under visible light irradiation

    Science.gov (United States)

    Rahman, Shakeelur; Momin, Bilal; Higgins M., W.; Annapure, Uday S.; Jha, Neetu

    2018-04-01

    In recent times, low cost and metal free photocatalyts driven under visible light have attracted a lot of interest. One such photo catalyst researched extensively is bulk graphitic carbon nitride sheets. But the low surface area and weak mobility of photo generated electrons limits its photocatalytic performance in the visible light spectrum. Here we present the facile synthesis of ultrathin graphitic carbon nitride using a cost effective melamine precursor and its application in highly efficient photocatalytic dye degradation of Rhodamine B molecules. Compared to bulk graphitic carbon nitride, the synthesized ultrathin graphitic carbon nitride shows an increase in surface area, a a decrease in optical band gap and effective photogenerated charge separation which facilitates the harvest of visible light irradiation. Due to these optimal properties of ultrathin graphitic carbon nitride, it shows excellent photocatalytic activity with photocatalytic degradation of about 95% rhodamine B molecules in 1 hour.

  19. Removal of rhodamine B (a basic dye) and thoron (an acidic dye) from dilute aqueous solutions and wastewater simulants by ion flotation.

    Science.gov (United States)

    Shakir, Kamal; Elkafrawy, Ahmed Faouzy; Ghoneimy, Hussein Fouad; Elrab Beheir, Shokry Gad; Refaat, Mamdoh

    2010-03-01

    The present work deals with removal, by ion flotation, of two dyes: a basic dye (rhodamine B (RB)) and an acidic one (thoron (TH)) from dilute aqueous solutions and simulated wastewaters. These dyes are widely used for analytical and biological staining purposes. Besides, RB is commonly used in dyeing of various industrial products. Therefore, wastewaters emanating from chemical and radiochemical laboratories, and biomedical and biological research laboratories may be contaminated with RB and TH. Ion flotation of these dyes has been investigated over a wide range of pH using the anionic surfactant, sodium lauryl sulfate (NaLS) and the cationic surfactant, cetyltrimethylammonium bromide (CTAB) as collectors. Successful removals could be achieved for RB and TH with the anionic collector, NaLS, and the cationic collector, CTAB, respectively. In addition to the effects of pH and type of collector on the efficiency of removal of each dye, the effects of collector and dye concentrations, frother dosage, ionic strength, bubbling time period and presence of foreign salts were investigated and the optimal removal conditions have been established. Removals exceeding 99.5 % and 99.9% could be achieved for RB and TH, respectively. The results obtained are discussed with respect to dissociation of dye, type of collector, ionic strength and sign and magnitude of charge of added foreign ions. Kinetics of flotation were also studied. Further studies demonstrate that under optimum conditions the developed flotation processes can be applied for the treatment of dye-contaminated wastewaters simulated to those generated at dyeing industries and radiochemical laboratories. Copyright 2009 Elsevier Ltd. All rights reserved.

  20. Study of the deposition features of the organic dye Rhodamine B on the porous surface of silicon with different pore sizes

    Energy Technology Data Exchange (ETDEWEB)

    Lenshin, A. S., E-mail: lenshinas@phys.vsu.ru; Seredin, P. V.; Kavetskaya, I. V.; Minakov, D. A.; Kashkarov, V. M. [Voronezh State University (Russian Federation)

    2017-02-15

    The deposition features of the organic dye Rhodamine B on the porous surface of silicon with average pore sizes of 50–100 and 100–250 nm are studied. Features of the composition and optical properties of the obtained systems are studied using infrared and photoluminescence spectroscopy. It is found that Rhodamine-B adsorption on the surface of por-Si with various porosities is preferentially physical. The optimal technological parameters of its deposition are determined.

  1. Stimulation of mitochondrial respiration induced by laser irradiation in the presence of rhodamine dyes

    International Nuclear Information System (INIS)

    Krasnikov, B.F.; Zorov, D.B.

    1996-01-01

    The effect of micromolar concentration of rhodamine 123 (methylrhodamine) and ethyl and amyl esters of unsubstituted rhodamine on oxygen consumption by rat liver mitochondria was studied under irradiation by an argon laser (488 and 514 nm). Irradiation of mitochondria in the presence of rhodamine stimulates their respiration. Light-induced stimulation of respiration is not inhibited by free radical scavenger ionol and by inhibitor of the permeability transition pore cyclosporine A. Stimulation of respiration by moderate doses of radiation is reversed in the dark. Increase in radiation dose resulted in only partial reversal of stimulated respiration in the dark. Rhodamine efficacy in stimulation of mitochondrial respiration depends on its structure (amyl > ethyl > methylrhodamine). 22 refs.; 4 figs

  2. Adsorption of rhodamine B and methylene blue dyes using waste of seeds of Aleurites Moluccana, a low cost adsorbent

    Directory of Open Access Journals (Sweden)

    Debora Luiza Postai

    2016-06-01

    Full Text Available Removal of the cationic dyes rhodamine B (RhB and methylene blue (MB by waste seeds Aleurites moluccana (WAM was studied in a batch system. The adsorbent was characterized by Fourier transform infrared spectroscopy (FTIR, thermogravimetric analysis (TGA, point of zero charge measurement, and the Boehm titration method. The effects of contact time and pH were investigated for the removal of cationic dyes. An increase in pH from 3 to 9 was accompanied by an approximately three-fold increase in the amount of dye adsorbed. The adsorptions equilibrium values were obtained and analyzed using the Langmuir, Freundlich, Sips, and Redlich–Peterson equations, the Sips isotherm being the one that showed the best correlation with the experimental values. The maximum adsorption capacities of the dyes were 178 mg/g for the MB and 117 mg/g for the RhB. The kinetic sorption was evaluated by the pseudo-first-order, pseudo-second-order, and intraparticle diffusion models, where it was observed that sorption follows the pseudo-second-order kinetic model. The study of thermodynamics showed that the adsorption is a spontaneous and endothermic process. The results indicate that waste seeds of A. moluccana could be used as a low cost material for the removal of cationic dyes from wastewater.

  3. Real-time piscicide tracking using Rhodamine WT dye for support of application, transport, and deactivation strategies in riverine environments

    Science.gov (United States)

    Jackson, Patrick Ryan; Lageman, Jonathan D.

    2013-01-01

    Piscicide applications in riverine environments are complicated by the advection and dispersion of the piscicide by the flowing water. Proper deactivation of the fish toxin is required outside of the treatment reach to ensure that there is minimal collateral damage to fisheries downstream or in connecting and adjacent water bodies. In urban settings and highly managed waterways, further complications arise from the influence of industrial intakes and outfalls, stormwater outfalls, lock and dam operations, and general unsteady flow conditions. These complications affect the local hydrodynamics and ultimately the transport and fate of the piscicide. This report presents two techniques using Rhodamine WT dye for real-time tracking of a piscicide plume—or any passive contaminant—in rivers and waterways in natural and urban settings. Passive contaminants are those that are present in such low concentration that there is no effect (such as buoyancy) on the fluid dynamics of the receiving water body. These methods, when combined with data logging and archiving, allow for visualization and documentation of the application and deactivation process. Real-time tracking and documentation of rotenone applications in rivers and urban waterways was accomplished by encasing the rotenone plume in a plume of Rhodamine WT dye and using vessel-mounted submersible fluorometers together with acoustic Doppler current profilers (ADCP) and global positioning system (GPS) receivers to track the dye and map the water currents responsible for advection and dispersion. In this study, two methods were used to track rotenone plumes: (1) simultaneous injection of dye with rotenone and (2) delineation of the upstream and downstream boundaries of the treatment zone with dye. All data were logged and displayed on a shipboard laptop computer, so that survey personnel provided real-time feedback about the extent of the rotenone plume to rotenone application and deactivation personnel. Further

  4. Spectroscopic insights on selfassembly and excited state interactions between rhodamine and phthalocyanine molecules.

    Science.gov (United States)

    Geng, Hao; Zhang, Xian-Fu

    2015-03-15

    The absorption and fluorescence spectra as well as fluorescence lifetimes of tetrasulfonated zinc phthalocyanine ZnPc(SO3Na)4 were measured in the absence and presence of four rhodamine dyes, Rhodamine B (RB), Ethyl rhodamine B (ERB), Rhodamine 6G (R6G), Rhodamine 110 (R110), and Pyronine B (PYB). The ground state complexes of phthalocyanine-(Rhodamine)2 were observed which exhibit new absorption bands. The binding constants are all very large (0.86×10(5)-0.22×10(8) M(-1)), suggesting rhodamine-phthalocyanine pairs are very good combinations for efficient selfassembly. Both the fluorescence intensity and the lifetime values of ZnPc(SO3Na)4 were decreased by the presence of rhodamines. The structural effect of rhodamines on selfassembly is significant. The ground state binding and dynamic quenching capability is PYB>R6G>ERB>RB>R110. The dynamic fluorescence quenching is due to the photoinduced electron transfer (PET). The PET rate constant is very large and in the order of 10(13) M(-1) s(-1), much greater than kf and kic (in the order of 10(8) M(-1) s(-1)), which means that the PET efficiency is almost 100%. Therefore the non-covalent Pc-rhodamine is a very good pair of donor/acceptor for potential efficient solar energy conversion. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Surface-enhanced resonance Raman scattering spectroscopy of single R6G molecules

    Institute of Scientific and Technical Information of China (English)

    Zhou Zeng-Hui; Liu Li; Wang Gui-Ying; Xu Zhi-Zhan

    2006-01-01

    Surface-enhanced resonance Raman scattering (SERRS) of Rhodamine 6G (R6G) adsorbed on colloidal silver clusters has been studied. Based on the great enhancement of the Raman signal and the quench of the fluorescence, the SERRS spectra of R6G were recorded for the samples of dye colloidal solution with different concentrations. Spectral inhomogeneity behaviours from single molecules in the dried sample films were observed with complementary evidences, such as spectral polarization, spectral diffusion, intensity fluctuation of vibrational lines and even "breathing" of the molecules. Sequential spectra observed from a liquid sample with an average of 0.3 dye molecules in the probed volume exhibited the expected Poisson distribution for actually measuring 0, 1 or 2 molecules. Difference between the SERRS spectra of R6G excited by linearly and circularly polarized light were experimentally measured.

  6. Polar red-emitting rhodamine dyes with reactive groups: synthesis, photophysical properties, and two-color STED nanoscopy applications.

    Science.gov (United States)

    Kolmakov, Kirill; Wurm, Christian A; Meineke, Dirk N H; Göttfert, Fabian; Boyarskiy, Vadim P; Belov, Vladimir N; Hell, Stefan W

    2014-01-03

    The synthesis, reactivity, and photophysical properties of new rhodamines with intense red fluorescence, two polar residues (hydroxyls, primary phosphates, or sulfonic acid groups), and improved hydrolytic stability of the amino-reactive sites (NHS esters or mixed N-succinimidyl carbonates) are reported. All fluorophores contain an N-alkyl-1,2-dihydro-2,2,4-trimethylquinoline fragment, and most of them bear a fully substituted tetrafluoro phenyl ring with a secondary carboxamide group. The absorption and emission maxima in water are in the range of 635-639 and 655-659 nm, respectively. A vastly simplified approach to red-emitting rhodamines with two phosphate groups that are compatible with diverse functional linkers was developed. As an example, a phosphorylated dye with an azide residue was prepared and was used in a click reaction with a strained alkyne bearing an N-hydroxysuccinimid (NHS) ester group. This method bypasses the undesired activation of phosphate groups, and gives an amphiphilic amino-reactive dye, the solubility and distribution of which between aqueous and organic phases can be controlled by varying the pH. The presence of two hydroxyl groups and a phenyl ring with two carboxyl residues in the dyes with another substitution pattern is sufficient for providing the hydrophilic properties. Selective formation of a mono-N-hydroxysuccinimidyl ester from 5-carboxy isomer of this rhodamine is reported. The fluorescence quantum yields varied from 58 to 92% for free fluorophores, and amounted to 18-64% for antibody conjugates in aqueous buffers. The brightness and photostability of these fluorophores facilitated two-color stimulated emission depletion (STED) fluorescence nanoscopy of biological samples with high contrast and minimal background. Selecting a pair of fluorophores with absorption/emission bands at 579/609 and 635/655 nm enabled two-color channels with low cross-talk and negligible background at approximately 40 nm resolution. Copyright

  7. Levitated droplet dye laser

    DEFF Research Database (Denmark)

    Azzouz, H.; Alkafadiji, L.; Balslev, Søren

    2006-01-01

    a high quality optical resonator. Our 750 nL lasing droplets consist of Rhodamine 6G dissolved in ethylene glycol, at a concentration of 0.02 M. The droplets are optically pumped at 532 nm light from a pulsed, frequency doubled Nd:YAG laser, and the dye laser emission is analyzed by a fixed grating...

  8. Graphene oxide based CdSe photocatalysts: Synthesis, characterization and comparative photocatalytic efficiency of rhodamine B and industrial dye

    International Nuclear Information System (INIS)

    Ghosh, Trisha; Lee, Jeong-Ho; Meng, Ze-Da; Ullah, Kefayat; Park, Chong-Yeon; Nikam, Vikram; Oh, Won-Chun

    2013-01-01

    Highlights: ► CdSe–graphene is synthesized by hydrothermal method. ► Three molar solutions of CdSe were used making three different composites. ► RhB and Texbrite MST-L were used as sample dye solutions. ► Texbrite MST-L is photo degraded in visible light. ► UV-spectroscopic analysis was done to measure degradation. - Abstract: CdSe–graphene composites were prepared using simple “hydrothermal method” where the graphene surface was modified using different molar solutions of cadmium selenide (CdSe) in aqueous media. The characterization of CdSe–graphene composites were studied by X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscope (SEM), and with transmission electron microscope (TEM). The catalytic activities of CdSe-composites were evaluated by degradation of rhodamine B (RhB) and commercial industrial dye “Texbrite MST-L (TXT-MST)” with fixed concentration. The degradation was observed by the decrease in the absorbance peak studied by UV spectrophotometer. The decrease in the dye concentration indicated catalytic degradation effect by CdSe–graphene composites

  9. Kinetics, isothermal and thermodynamics studies of electrocoagulation removal of basic dye rhodamine B from aqueous solution using steel electrodes

    Science.gov (United States)

    Adeogun, Abideen Idowu; Balakrishnan, Ramesh Babu

    2017-07-01

    Electrocoagulation was used for the removal of basic dye rhodamine B from aqueous solution, and the process was carried out in a batch electrochemical cell with steel electrodes in monopolar connection. The effects of some important parameters such as current density, pH, temperature and initial dye concentration, on the process, were investigated. Equilibrium was attained after 10 min at 30 °C. Pseudo-first-order, pseudo-second-order, Elovich and Avrami kinetic models were used to test the experimental data in order to elucidate the kinetic adsorption process; pseudo-first-order and Avrami models best fitted the data. Experimental data were analysed using six model equations: Langmuir, Freudlinch, Redlich-Peterson, Temkin, Dubinin-Radushkevich and Sips isotherms and it was found that the data fitted well with Sips isotherm model. The study showed that the process depends on current density, temperature, pH and initial dye concentration. The calculated thermodynamics parameters (Δ G°, Δ H° and Δ S°) indicated that the process is spontaneous and endothermic in nature.

  10. Adsorption properties of cationic rhodamine B dye onto metals chloride-activated castor bean residue carbons.

    Science.gov (United States)

    Zhi, Lee Lin; Zaini, Muhammad Abbas Ahmad

    2017-02-01

    This work was aimed to evaluate the feasibility of castor bean residue based activated carbons prepared through metals chloride activation. The activated carbons were characterized for textural properties and surface chemistry, and the adsorption data of rhodamine B were established to investigate the removal performance. Zinc chloride-activated carbon with specific surface area of 395 m 2 /g displayed a higher adsorption capacity of 175 mg/g. Magnesium chloride and iron(III) chloride are less toxic and promising agents for composite chemical activation. The adsorption data obeyed Langmuir isotherm and pseudo-second-order kinetics model. The rate-limiting step in the adsorption of rhodamine B is film diffusion. The positive values of enthalpy and entropy indicate that the adsorption is endothermic and spontaneous at high temperature.

  11. Fluorescein isothiocyanate and rhodamine B dye encapsulated mesoporous SiO2 for applications of blue LED excited white LED

    Science.gov (United States)

    Das, Sourav; Manam, J.

    2018-05-01

    In this work, the fluorescein isothiocyanate (FITC) and rhodamine B (RhB) dyes were encapsulated in mesoporous silica nanoparticles (MSNp). The MSNp-FITC-RhB nanohybrids phosphor showed a dichromatic PL emission at green region and orange region when excited at 460 nm. A Forster Resonance Energy Transfer (FRET) was observed from FITC to RhB. The materials were further characterized by XRD, FTIR, TEM, and temperature dependent photoluminescence. The CIE coordinates were tuned from greenish yellow to the orange region and quantum yield was reached 52.04% based on FRET. So by combining the MSNp-FITC-RhB nanohybrids phosphor with the blue LED chip, the white light emission with flexible Color Correlated Temperature and improved Color Rendering Index can be obtained.

  12. Feasibility of solar-pumped dye lasers

    Science.gov (United States)

    Lee, Ja H.; Kim, Kyung C.; Kim, Kyong H.

    1987-01-01

    Dye laser gains were measured at various pump-beam irradiances on a dye cell in order to evaluate the feasibility of solar pumping. Rhodamine 6G dye was considered as a candidate for the solar-pumped laser because of its high utilization of the solar spectrum and high quantum efficiency. Measurements show that a solar concentration of 20,000 is required to reach the threshold of the dye.

  13. UV-visible spectroscopic estimation of photodegradation of rhodamine-B dye using tin(IV) oxide nanoparticles.

    Science.gov (United States)

    Sangami, G; Dharmaraj, N

    2012-11-01

    Nanocrystalline, tin(IV) oxide (SnO(2)) particles has been prepared by thermal decomposition of tin oxalate precursor obtained from the reactions of tin(IV) chloride and sodium oxalate using eggshell membrane (ESM). The as-prepared SnO(2) nanoparticles were characterized by thermal studies, transmission electron microscopy (TEM), powder X-ray diffraction (XRD), Raman, FT-IR and UV-visible studies and used as a photocatalyst for the degradation of rhodamine-B (Rh-B) dye. The size of the prepared nanoparticles was in the range of 5-12nm as identified from the TEM images. Powder XRD data revealed the presence of a tetragonal, rutile crystalline phase of the tin(IV) oxide nanoparticles. Thermal analysis showed that the decomposition of tin oxalate precursor to yield the titled tin(IV) oxide nanoparticles was completed below 500°C. The extent of degradation of Rh-B in the presence of SnO(2) monitored by absorption spectral measurements demonstrated that 94.48% of the selected dye was degraded upon irradiation with UV light for 60 min. Copyright © 2012 Elsevier B.V. All rights reserved.

  14. Photocatalytic Degradation of Rhodamine B Dye over Novel Porous TiO2-SnO2 Nanocomposites Prepared by Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    Yan Wang

    2014-01-01

    Full Text Available The photocatalytic degradation of Rhodamine B dye was successfully carried out under UV irradiation over porous TiO2-SnO2 nanocomposites with various molar ratios of Ti/Sn (4–12 synthesized by hydrothermal method using polystyrene microspheres as template. The combination of TiO2 with SnO2 can obtain high quantum yield of TiO2, and then achieve the high photocatalytic activity. And its porous structure can provide large surface area, leading to more adsorption and fast transfer of dye pollutant. Structural and textural features of the samples were investigated by X-ray diffraction (XRD, transmission electron microscopy (TEM, and N2 sorption techniques. Both adsorption and UV irradiation contribute to decolorization of about 100% of Rhodamine B dye over the sample TiSn10 after 30 min of the photocatalytic reaction, while the decomposition of Rhodamine B dye is only 62% over pure titania (Degussa P25.

  15. Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications.

    OpenAIRE

    Belov, V.; Mitronova, G.; Bossi, M.; Boyarski, V.; Hebisch, E.; Geisler, C.; Kolmakov, K.; Wurm, C.; Willig, K.; Hell, S.

    2014-01-01

    Caged rhodamine dyes (Rhodamines NN) of five basic colors were synthesized and used as “hidden” markers in subdiffractional and conventional light microscopy. These masked fluorophores with a 2-diazo-1-indanone group can be irreversibly photoactivated, either by irradiation with UV- or violet light (one-photon process), or by exposure to intense red light (λ∼750 nm; two-photon mode). All dyes possess a very small 2-diazoketone caging group incorporated into the 2-diazo-1-indanone residue with...

  16. Micro-fabricated solid state dye lasers based on a photo-definable polymer

    DEFF Research Database (Denmark)

    Nilsson, Daniel; Balslev, Søren; Gregersen, Misha Marie

    2005-01-01

    the commercially available laser dye Rhodamine 6G, which is incorporated into the SU-8 polymer matrix. The single-mode slab waveguide is formed by three-step spin-coating deposition: a buffer layer of undoped SU-8, a core layer of SU-8 doped with Rhodamine, and a cladding layer of undoped SU-8. (c) 2005 Optical...

  17. Investigation of the dye concentration influence on the lasing wavelength and threshold for a micro-fluidic dye laser

    DEFF Research Database (Denmark)

    Helbo, Bjarne; Kragh, Søren; Kjeldsen, B.G.

    2003-01-01

    We investigate a micro-fluidic dye laser, which can be integrated with polymer-based lab-on-a-chip microsystems without further processing steps. A simple rate-equation model is used to predict the lasing threshold. The laser device is characterised using the laser dye Rhodamine 6G dissolved...... in ethanol, and the influence of dye concentration on the lasing wavelength and threshold is investigated. The experiments confirm the predictions of the rate-equation model, that lasing can be achieved in the 10 mum long laser cavity with moderate concentrations of Rhodamine 6G in ethanol, starting from 5 x...

  18. Enhancing the photovoltaic performance of bulk heterojunction polymer solar cells by adding Rhodamine B laser dye as co-sensitizer.

    Science.gov (United States)

    Kazemifard, Sholeh; Naji, Leila; Afshar Taromi, Faramarz

    2018-04-01

    Ternary blend (TB) strategy has been considered as an effective method to enhance the photovoltaic performance of bulk heterojunction (BHJ) polymer solar cells (PSCs). Here, we report on TB-based PSCs containing two donor materials; poly-3-hexylthiophene (P3HT) and Rhodamine B (RhB) laser organic dye, and [6,6]-phenyl C 61 butyric acid methyl ester (PC 61 BM) as an acceptor. The influence of RhB weight percentage and injection volume was extensively studied. To gain insight into the influences of RhB on the photovoltaic performance of PSCs, physicochemical and optical properties of TBs were compared with those of BHJ binary blend as a standard. RhB broadened the light absorption properties of the active layer and played a bridging role between P3HT and PC 61 BM. The PCE and short-circuit current density (Jsc) of the optimized TB-based PSCs comprising of 0.5 wt% RhB reached 5% and 12.12 mA/cm 2 , respectively. Compared to BHJ standard cell, the PCE and the generated current was improved by two orders of magnitude due to higher photon harvest of the active layer, cascade energy level structure of TB components and a considerable decrease in the charge carrier recombination. The results suggest that RhB can be considered as an effective material for application in PSCs to attain high photovoltaic performance. Copyright © 2018 Elsevier Inc. All rights reserved.

  19. LASERS, ACTIVE MEDIA: The aqueous-polyelectrolyte dye solution as an active laser medium

    Science.gov (United States)

    Akimov, A. I.; Saletskii, A. M.

    2000-11-01

    The spectral, luminescent, and lasing properties of aqueous solutions of a cationic dye rhodamine 6G with additions of anion polyelectrolytes — polyacrylic and polymethacrylic acids — are studied. It is found that the energy and spectral properties of lasing of these solutions depend on the ratio of concentrations of polyelectrolyte and molecules. It is also found that the lasing parameters of aqueous-polyelectrolyte dye solutions can be controlled by changing the structure of the molecular system. The variation in the structure of aqueous-polyelectrolyte dye solutions of rhodamine 6G resulted in an almost five-fold increase in the lasing efficiency compared to that in aqueous dye solutions.

  20. Efficiencies of laser dyes for atomic vapor laser isotope separation

    International Nuclear Information System (INIS)

    Maeda, Mitsuo; Oki, Yuji; Uchiumi, Michihiro; Takao, Takayuki; Igarashi, Kaoru; Shimamoto, Kojiro.

    1995-01-01

    Efficiencies of 30 laser dyes for the atomic vapor laser isotope separation (AVLIS) are experimentally evaluated with a dye laser pumped by a frequency-doubled Nd:YAG laser. On the other hand, a simulation code is developed to describe the laser action of Rhodamine 6G, and the dependence of the laser efficiency on the pump wavelength is calculated. Following conclusions are obtained by these considerations:space: 1) Pyrromethene 567 showed 16% higher laser efficiency than Rhodamine 6G by 532 nm pumping, and Pyrromethene 556 has an ability to provide better efficiency by green light pumping with a Cu vapor laser; 2) Kiton red 620 and Rhodamine 640, whose efficiencies were almost the same as Rhodamine 6G by 532 nm pumping, will show better efficiencies by two-wavelength pumping with a Cu vapor laser. (author)

  1. Argon laser phototherapy of human malignancies using rhodamine-123 as a new laser dye: The intracellular role of oxygen

    International Nuclear Information System (INIS)

    Castro, D.J.; Saxton, R.E.; Markley, J.; Foote, C.S.; Fetterman, H.R.; Castro, D.J.; Ward, P.H.

    1990-01-01

    Recent studies demonstrated that the cationic, mitochondrial-specific dye Rhodamine-123 (Rh-123), is an efficient tumor photosensitizer for Argon laser treatment of human cancer cells both in vitro and in tumors grown as xenografts in athymic mice. To demonstrate the photodynamic mechanism of action of this reaction, the intracellular role of oxygen and temperature changes in treated cells have to be defined. In the current study, a large panel of human tumor cell lines of diverse histologic origin were tested for in vitro sensitivity to Rh-123 and the Argon laser (514.5 nm) in oxygen, deuterium oxide (D2O), and nitrogen (N2) environment. Tumor cells in suspension were first sensitized to Rh-123 (1 or 20 micrograms/ml for 1 hour), cooled on ice to 4 degrees C, and then exposed to the Argon laser (delta T = 14 +/- 1 degree C). Cell proliferation measured by [3H]-thymidine uptake 24 hours after sensitization with Rh-123 and laser treatment was significantly decreased in tumor cells kept in oxygen and D2O atmospheres. No decrease in DNA synthesis was seen in Rh-123 and laser treated cells kept in an N2 environment. Control tumor cells treated with Rh-123 or the Argon laser separately did not show any decreased [3H]-thymidine uptake in oxygen, D2O or N2 environment. These results provide evidence of a photodynamic process since Rh-123 sensitization and Argon laser activation occur at nonthermal levels of energy and are oxygen dependent. The high effectiveness of this technique of photodynamic therapy with the Argon laser, and low toxicity of Rh-123 could make its clinical use very attractive for the treatment of superficial malignancies

  2. Optical study of planar waveguides based on oxidized porous silicon impregnated with laser dyes

    Energy Technology Data Exchange (ETDEWEB)

    Chouket, A. [Unite de recherche de Spectroscopie Raman, Departement de Physique, Faculte des Sciences de Tunis, Elmanar 2092, Tunis (Tunisia); Charrier, J. [Laboratoire d' Optronique CNRS-UMR FOTON 6082, Universite de Rennes 1, ENSSAT-6 rue de Kerampont, BP 80518, 22305 Lannion Cedex (France); Elhouichet, H. [Unite de recherche de Spectroscopie Raman, Departement de Physique, Faculte des Sciences de Tunis, Elmanar 2092, Tunis (Tunisia)], E-mail: habib.elhouichet@fst.rnu.tn; Oueslati, M. [Unite de recherche de Spectroscopie Raman, Departement de Physique, Faculte des Sciences de Tunis, Elmanar 2092, Tunis (Tunisia)

    2009-05-15

    Oxidized porous silicon optical planar waveguides were elaborated and impregnated with rhodamine B and rhodamine 6G. The waveguiding, absorption, and photoluminescence properties of these impregnated waveguides were studied. Successful impregnation of the structure with laser dyes is shown from photoluminescence and reflectivity measurements. Furthermore, the reflectivity spectra prove the homogenous incorporation of both dye molecules inside the pores of the matrices. The refractive indices of waveguide layers were determined before and after dye impregnation to indicate the conservation of guiding conditions. The optical losses in the visible wavelengths are studied as a function of dye concentration. The dye absorption is the main reason for these losses.

  3. Detection of gain enhancement in laser-induced fluorescence of rhodamine B lasing dye by silicon dioxide nanostructures-coated cavity

    Science.gov (United States)

    Al-Tameemi, Mohammed N. A.

    2018-03-01

    In this work, nanostructured silicon dioxide films are deposited by closed-field unbalanced direct-current (DC) reactive magnetron sputtering technique on two sides of quartz cells containing rhodamine B dye dissolved in ethanol with 10‒5 M concentration as a random gain medium. The preparation conditions are optimized to prepare highly pure SiO2 nanostructures with a minimum particle size of about 20 nm. The effect of SiO2 films as external cavity for the random gain medium is determined by the laser-induced fluorescence of this medium, and an increase of about 200% in intensity is observed after the deposition of nanostructured SiO2 thin films on two sides of the dye cell.

  4. Bibliographic study of photophysical and photochemical properties of laser dyes

    International Nuclear Information System (INIS)

    Doizi, D.

    1986-06-01

    Laser isotope separation of uranium requires high power and precise wave length. This report is a bibliographic and experimental study of the photophysical and photochemical properties of seven commercial laser dyes which have an emission wavelength in the range 5500-6500 A: Rhodamine 110 or 560, rhodamine 6G or 590, rhodamine B or 610, rhodamine 101 or 640, sulforhodamine B or kiton red 620, sulforhodamine 101 or 640 and DCM or LC 6500. Absorption and emission cross section values, fluorescence lifetimes and quantum yields in various solvents are indicated. For each dye, a non exhaustive list of laboratory experiments made with two types of pump sources: Nd YAG (532) and copper vapor laser is given. When it is known, the toxicity of the dyes is mentioned [fr

  5. Micro-Cavity Fluidic Dye Laser

    DEFF Research Database (Denmark)

    Helbo, Bjarne; Kristensen, Anders; Menon, Aric Kumaran

    2003-01-01

    We have successfully designed, fabricated and characterized a micro-cavity fluidic dye laser with metallic mirrors, which can be integrated with polymer based lab-on-a-chip microsystems without further processing steps. A simple rate-equation model is used to predict the average pumping power...... threshold for lasing as function of cavity-mirror reflectance, laser dye concentration and cavity length. The laser device is characterized using the laser dye Rhodamine 6G dissolved in ethanol. Lasing is observed, and the influence of dye concentration is investigated....

  6. Modification of fluorescence and optical properties of Rhodamine B dye doped PVA/Chitosan polymer blend films

    Science.gov (United States)

    Padmakumari, R.; Ravindrachary, V.; Mahantesha, B. K.; Sagar, Rohan N.; Sahanakumari, R.; Bhajantri, R. F.

    2018-05-01

    Pure and Rhodamine B doped Poly (vinyl alcohol)/Chitosan composite films are prepared using solution casting method. Fourier transforms infrared spectra (FTIR), Ultraviolet-Visible (UV-Vis), fluorescence studies were used to characterize the prepared polymer films. The FT-IR results show that the appearance of new peaks along with shift in peak positions indicates the interaction of Rhodamine B with PVA-CS blend. Optical absorption edge, band gap and activation energy were determined from UV-Visible studies. The optical absorption edge increases, band gap decreases and activation energy increases with dopant concentration respectively. The corresponding emission spectra were studied using fluorescence spectroscopy. From the fluorescence study the quenching phenomena are observed in emission wavelength range of 607nm-613nm upon excitation with absorption maxima 443nm.

  7. Coupling carbon nanotube film microextraction with desorption corona beam ionization for rapid analysis of Sudan dyes (I-IV) and Rhodamine B in chilli oil.

    Science.gov (United States)

    Chen, Di; Huang, Yun-Qing; He, Xiao-Mei; Shi, Zhi-Guo; Feng, Yu-Qi

    2015-03-07

    A rapid analysis method by coupling carbon nanotube film (CNTF) microextraction with desorption corona beam ionization (DCBI) was developed for the determination of Sudan dyes (I-IV) and Rhodamine B in chilli oil samples. Typically, CNTF was immersed into the diluted solution of chilli oil for extraction, which was then placed directly under the visible plasma beam tip of the DCBI source for desorption and ionization. Under optimized conditions, five dyes were simultaneously determined using this method. Results showed that the analytes were enriched by the CNTF through the π-π interactions, and the proposed method could significantly improve the sensitivities of these compounds, compared to the direct analysis by DCBI-MS/MS. The method with a linear range of 0.08-12.8 μg g(-1) and good linear relationships (R(2) > 0.93) in a multiple reaction monitoring (MRM) mode was developed. Satisfactory reproducibility was achieved. Relative standard deviations (RSDs) were less than 20.0%. The recoveries ranged from 80.0 to 110.0%, and the limits of detection (LODs) were in the range of 1.4-21 ng g(-1). Finally, the feasibility of the method was further exhibited by the determination of five illegal dyes in chilli powder. These results demonstrate that the proposed method consumes less time and solvent than conventional HPLC-based methods and avoids the contamination of chromatographic column and ion source from non-volatile oil. With the help of a 72-well shaker, multiple samples could be treated simultaneously, which ensures high throughput for the entire pretreatment process. In conclusion, it provides a rapid and high-throughput approach for the determination of such illicit additions in chilli products.

  8. Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications.

    Science.gov (United States)

    Belov, Vladimir N; Mitronova, Gyuzel Yu; Bossi, Mariano L; Boyarskiy, Vadim P; Hebisch, Elke; Geisler, Claudia; Kolmakov, Kirill; Wurm, Christian A; Willig, Katrin I; Hell, Stefan W

    2014-10-06

    Caged rhodamine dyes (Rhodamines NN) of five basic colors were synthesized and used as "hidden" markers in subdiffractional and conventional light microscopy. These masked fluorophores with a 2-diazo-1-indanone group can be irreversibly photoactivated, either by irradiation with UV- or violet light (one-photon process), or by exposure to intense red light (λ∼750 nm; two-photon mode). All dyes possess a very small 2-diazoketone caging group incorporated into the 2-diazo-1-indanone residue with a quaternary carbon atom (C-3) and a spiro-9H-xanthene fragment. Initially they are non-colored (pale yellow), non-fluorescent, and absorb at λ=330-350 nm (molar extinction coefficient (ε)≈10(4)  M(-1)  cm(-1)) with a band edge that extends to about λ=440 nm. The absorption and emission bands of the uncaged derivatives are tunable over a wide range (λ=511-633 and 525-653 nm, respectively). The unmasked dyes are highly colored and fluorescent (ε=3-8×10(4)  M(-1)  cm(-1) and fluorescence quantum yields (ϕ)=40-85% in the unbound state and in methanol). By stepwise and orthogonal protection of carboxylic and sulfonic acid groups a highly water-soluble caged red-emitting dye with two sulfonic acid residues was prepared. Rhodamines NN were decorated with amino-reactive N-hydroxysuccinimidyl ester groups, applied in aqueous buffers, easily conjugated with proteins, and readily photoactivated (uncaged) with λ=375-420 nm light or intense red light (λ=775 nm). Protein conjugates with optimal degrees of labeling (3-6) were prepared and uncaged with λ=405 nm light in aqueous buffer solutions (ϕ=20-38%). The photochemical cleavage of the masking group generates only molecular nitrogen. Some 10-40% of the non-fluorescent (dark) byproducts are also formed. However, they have low absorbance and do not quench the fluorescence of the uncaged dyes. Photoactivation of the individual molecules of Rhodamines NN (e.g., due to reversible or irreversible

  9. Single-mode solid-state polymer dye laser fabricated with standard I-line UV lithography

    DEFF Research Database (Denmark)

    Balslev, Søren; Mironov, Andrej; Nilsson, Daniel

    2005-01-01

    We present single-mode solid-state polymer dye lasers fabricated with standard UV lithography. The lasers use a high-order Bragg grating and rely on index-tuning of a photosensitive polymer for waveguiding. The gain medium is Rhodamine 6G.......We present single-mode solid-state polymer dye lasers fabricated with standard UV lithography. The lasers use a high-order Bragg grating and rely on index-tuning of a photosensitive polymer for waveguiding. The gain medium is Rhodamine 6G....

  10. Synthesis and characterisation of new laser dyes

    International Nuclear Information System (INIS)

    Scala-Valero, Claudine

    1997-01-01

    Rhodamines are very efficient laser dyes for the red part of the visible spectrum: their fluorescence quantum efficiencies are about hundred per cent. However, their conversion efficiencies in dye amplifier are about fifty per cent, due to the presence of S n electronic level which is responsible of the re-absorption of a part of photons. In this research thesis, the author aims at trying to move this S n level out of the stimulated emission bandwidth. Models have been developed to propose new structures derived from rhodamines and theoretically possessing the desired properties. The so-recommended molecules have then been synthesised and characterised in terms of absorption and fluorescence spectra, of quantum efficiency, and of fluorescence lifetime. Two modifications are proposed for the rhodamine 6G structure, either by grafting methyl substitutes, or by grafting variously substituted amines. The searched result is obtained with the second modification [fr

  11. Threshold pump power of a solar-pumped dye laser

    Science.gov (United States)

    Lee, Ja H.; Kim, Kyung C.; Kim, Kyong H.

    1988-01-01

    Threshold solar power for dye laser pumping has been determined by measuring the gain of a rhodamine 6G dye laser amplifier at various solar-simulated irradiances on an amplifier cell. The measured threshold was 20,000 solar constants (2.7 kW/sq cm) for the dye volume of 2 x 5 x 40 cu mm and the optimum dye concentration of 0.001 M. The threshold is about one-third of that achievable with a high-intensity solar concentrator.

  12. Single mode solid state distributed feedback dye laser fabricated by grey scale electron beam lithography on dye doped SU-8 resist

    DEFF Research Database (Denmark)

    Balslev, Søren; Rasmussen, Torben; Shi, Peixiong

    2005-01-01

    We demonstrate grey scale electron beam lithography on functionalized SU-8 resist for fabrication of single mode solid state dye laser devices. The resist is doped with Rhodamine 6G perchlorate and the lasers are based on a first order Bragg grating distributed feedback resonator. The lasers...

  13. Activatable Optical Imaging with a Silica-Rhodamine Based Near Infrared (SiR700) Fluorophore: A comparison with cyanine based dyes

    Science.gov (United States)

    McCann, Thomas E.; Kosaka, Nobuyuki; Koide, Yuichiro; Mitsunaga, Makoto; Choyke, Peter L.; Nagano, Tetsuo; Urano, Yasuteru; Kobayashi, Hisataka

    2011-01-01

    Optical imaging is emerging as an important tool to visualize tumors. However, there are many potential choices among the available fluorophores. Optical imaging probes that emit in the visible range can image superficial tumors with high quantum yields, however, if deeper imaging is needed then near infrared (NIR) fluorophores are necessary. Most commercially available NIR fluorophores are cyanine based and are prone to non-specific binding and relatively limited photostability. Silica-containing rhodamine (SiR) fluorophores represent a new class of NIR fluorophores, which permit photoactivation via H-dimer formation as well as demonstrate improved photostability. This permits higher tumor-to-background ratios (TBRs) to be achieved over longer periods of time. Here, we compared an avidin conjugated with SiR700 (Av-SiR700) to similar compounds based on cyanine dyes (Av-Cy5.5 and Av-Alexa Fluor 680) in a mouse tumor model of ovarian cancer metastasis. We found that the Av-SiR700 probe demonstrated superior quenching enabling activation after binding-internalization to the target cell. As a result, Av-SiR700 had higher TBRs compared to Av-Cy5.5, and better biostability compared to Av-Alexa Fluor 680. PMID:22034863

  14. How to prevent dimerization of laser dyes in water? Simulation and organic synthesis

    International Nuclear Information System (INIS)

    Dare-Doyen, S.

    2000-01-01

    Xanthenes are widely used as laser dyes in ethanol medium because their photophysical properties there are excellent. On the other hand, when they are dissolved in water, their fluorescence is almost zero on account of the dimerization phenomenon (aggregation of two molecules) which is specific in water although the interaction between the two molecules (these dyes are mainly cations) be repulsive. The first part of this work deals with the dimerization study of two dyes, the 6G rhodamine and the 6G pyronine. Molecular dynamics simulation results (AMBER software) have been compared with those of the NMR; thus it has been possible to describe the geometry of the 6G rhodamine dimer and to identify two structures present in equal quantities for the 6G pyronine dimer. It has been demonstrated that the role of water is essential in the aggregation mechanism; this role can be understood as resulting of the hydrophobic effect. The second part of this work concerns the synthesis of rhodamines which are soluble but not able to dimerize in water at the running concentrations of the laser dyes. At first, aminophenol precursors having hydrophilic ionic groups on modifiable sites have been synthesized without changing their photophysical properties. The synthesis sequence of the 3-(2-alkylamino-4-hydroxyphenyl)propionic acids has not given the waited products but N-(3'-hydroxyphenyl)amino-alkylsulfonic acids have been obtained. Their condensation with the phthalic anhydride has led to dyes of a charge -2 at a pH of 5 in water and which have photophysical properties similar to those of the rhodamine 575 in ethanol and laser emission properties in the emission spectral range of the rhodamine 6G in ethanol. This synthesis work has then led to the preparation of two laser dyes usable in water. (author) [fr

  15. Hydrophobic analogues of rhodamine B and rhodamine 101: potent fluorescent probes of mitochondria in living C. elegans

    Directory of Open Access Journals (Sweden)

    Laurie F. Mottram

    2012-12-01

    Full Text Available Mitochondria undergo dynamic fusion and fission events that affect the structure and function of these critical energy-producing cellular organelles. Defects in these dynamic processes have been implicated in a wide range of human diseases including ischemia, neurodegeneration, metabolic disease, and cancer. To provide new tools for imaging of mitochondria in vivo, we synthesized novel hydrophobic analogues of the red fluorescent dyes rhodamine B and rhodamine 101 that replace the carboxylate with a methyl group. Compared to the parent compounds, methyl analogues termed HRB and HR101 exhibit slightly red-shifted absorbance and emission spectra (5–9 nm, modest reductions in molar extinction coefficent and quantum yield, and enhanced partitioning into octanol compared with aqueous buffer of 10-fold or more. Comparison of living C. elegans (nematode roundworm animals treated with the classic fluorescent mitochondrial stains rhodamine 123, rhodamine 6G, and rhodamine B, as well as the structurally related fluorophores rhodamine 101, and basic violet 11, revealed that HRB and HR101 are the most potent mitochondrial probes, enabling imaging of mitochondrial motility, fusion, and fission in the germline and other tissues by confocal laser scanning microscopy after treatment for 2 h at concentrations as low as 100 picomolar. Because transgenes are poorly expressed in the germline of these animals, these small molecules represent superior tools for labeling dynamic mitochondria in this tissue compared with the expression of mitochondria-targeted fluorescent proteins. The high bioavailabilty of these novel fluorescent probes may facilitate the identification of agents and factors that affect diverse aspects of mitochondrial biology in vivo.

  16. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats.

    Science.gov (United States)

    Jiang, Shiau-Han; Cheng, Yung-Yi; Huo, Teh-Ia; Tsai, Tung-Hu

    2017-09-06

    Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, and sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats. Rhodamine 110 exhibited linear pharmacokinetics and slow elimination after oral administration. Furthermore, its oral bioavailability was approximately 34-35%. The distribution in the liver and kidney suggests that these organs are primarily responsible for rhodamine 110 metabolism and elimination. Our investigation describes the pharmacokinetics and a quantification method for rhodamine 110, improving our understanding of the food safety of rhodamine dyes.

  17. Radioiodinated Rhodamine-123: a potential cationic hepatobiliary imaging agent

    International Nuclear Information System (INIS)

    Moonen, P.; Gorree, G.C.M.; Hoekstra, A.

    1987-01-01

    The labelling of the cationic dye Rhodamine-123 with 125 I is described. The biodistribution of the iodinated Rhodamine-123 has been determined at different time intervals after intravenous injection into fasted rats. It turned out that the dye is predominantly cleared by the liver and discharged into the bile. The bile acid taurocholate did not enhance the rate of excretion of 125 I-Rhodamine-123. (author)

  18. (18)F-labeled rhodamines as potential myocardial perfusion agents: comparison of pharmacokinetic properties of several rhodamines.

    Science.gov (United States)

    Bartholomä, Mark D; Zhang, Shaohui; Akurathi, Vamsidhar; Pacak, Christina A; Dunning, Patricia; Fahey, Frederic H; Cowan, Douglas B; Treves, S Ted; Packard, Alan B

    2015-10-01

    We recently reported the development of the [(18)F]fluorodiethylene glycol ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging (MPI). This compound was developed by optimizing the ester moiety on the rhodamine B core, and its pharmacokinetic properties were found to be superior to those of the prototype ethyl ester. The goal of the present study was to optimize the rhodamine core while retaining the fluorodiethyleneglycol ester prosthetic group. A series of different rhodamine cores (rhodamine 6G, rhodamine 101, and tetramethylrhodamine) were labeled with (18)F using the corresponding rhodamine lactones as the precursors and [(18)F]fluorodiethylene glycol ester as the prosthetic group. The compounds were purified by semipreparative HPLC, and their biodistribution was measured in rats. Additionally, the uptake of the compounds was evaluated in isolated rat cardiomyocytes. As was the case with the different prosthetic groups, we found that the rhodamine core has a significant effect on the in vitro and in vivo properties of this series of compounds. Of the rhodamines evaluated to date, the pharmacologic properties of the (18)F-labeled diethylene glycol ester of rhodamine 6G are superior to those of the (18)F-labeled diethylene glycol esters of rhodamine B, rhodamine 101, and tetramethylrhodamine. As with (18)F-labeled rhodamine B, [(18)F]rhodamine 6G was observed to localize in the mitochondria of isolated rat cardiomyocytes. Based on these results, the (18)F-labeled diethylene glycol ester of rhodamine 6G is the most promising potential PET MPI radiopharmaceutical of those that have evaluated to date, and we are now preparing to carry out first-in-human clinical studies with this compound. Copyright © 2015 Elsevier Inc. All rights reserved.

  19. 18F-labeled rhodamines as potential myocardial perfusion agents: comparison of pharmacokinetic properties of several rhodamines

    International Nuclear Information System (INIS)

    Bartholomä, Mark D.; Zhang, Shaohui; Akurathi, Vamsidhar; Pacak, Christina A.; Dunning, Patricia; Fahey, Frederic H.; Cowan, Douglas B.; Ted Treves, S.; Packard, Alan B.

    2015-01-01

    Introduction: We recently reported the development of the [ 18 F]fluorodiethylene glycol ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging (MPI). This compound was developed by optimizing the ester moiety on the rhodamine B core, and its pharmacokinetic properties were found to be superior to those of the prototype ethyl ester. The goal of the present study was to optimize the rhodamine core while retaining the fluorodiethyleneglycol ester prosthetic group. Methods: A series of different rhodamine cores (rhodamine 6G, rhodamine 101, and tetramethylrhodamine) were labeled with 18 F using the corresponding rhodamine lactones as the precursors and [ 18 F]fluorodiethylene glycol ester as the prosthetic group. The compounds were purified by semipreparative HPLC, and their biodistribution was measured in rats. Additionally, the uptake of the compounds was evaluated in isolated rat cardiomyocytes. Results: As was the case with the different prosthetic groups, we found that the rhodamine core has a significant effect on the in vitro and in vivo properties of this series of compounds. Of the rhodamines evaluated to date, the pharmacologic properties of the 18 F-labeled diethylene glycol ester of rhodamine 6G are superior to those of the 18 F-labeled diethylene glycol esters of rhodamine B, rhodamine 101, and tetramethylrhodamine. As with 18 F-labeled rhodamine B, [ 18 F]rhodamine 6G was observed to localize in the mitochondria of isolated rat cardiomyocytes. Conclusions: Based on these results, the 18 F-labeled diethylene glycol ester of rhodamine 6G is the most promising potential PET MPI radiopharmaceutical of those that have evaluated to date, and we are now preparing to carry out first-in-human clinical studies with this compound

  20. 18F-labeled Rhodamines as Potential Myocardial Perfusion Agents: Comparison of Pharmacokinetic Properties of Several Rhodamines

    Science.gov (United States)

    Bartholoma, Mark D.; Zhang, Shaohui; Akurathi, Vamsidhar; Pacak, Christina A.; Dunning, Patricia; Fahey, Frederic H.; Cowan, Douglas B.; Treves, S. Ted; Packard, Alan B.

    2015-01-01

    Introduction We recently reported the development of the [18F]fluorodiethylene glycol ester of rhodamine B as a potential positron emission tomography (PET) tracer for myocardial perfusion imaging (MPI). This compound was developed by optimizing the ester moiety on the rhodamine B core, and its pharmacokinetic properties were found to be superior to those of the prototype ethyl ester. The goal of the present study was to optimize the rhodamine core while retaining the fluorodiethyleneglycol ester prosthetic group. Methods A series of different rhodamine cores (rhodamine 6G, rhodamine 101, and tetramethylrhodamine) were labeled with 18F using the corresponding rhodamine lactones as the precursors and [18F]fluorodiethylene glycol ester as the prosthetic group. The compounds were purified by semipreparative HPLC, and their biodistribution was measured in rats. Additionally, the uptake of the compounds was evaluated in isolated rat cardiomyocytes. Results As was the case with the different prosthetic groups, we found that the rhodamine core has a significant effect on the in vitro and in vivo properties of this series of compounds. Of the rhodamines evaluated to date, the pharmacologic properties of the 18F-labeled diethylene glycol ester of rhodamine 6G are superior to those of the 18F-labeled diethylene glycol esters of rhodamine B, rhodamine 101, and tetramethylrhodamine. As with 18F-labeled rhodamine B, [18F]rhodamine 6G was observed to localize in the mitochondria of isolated rat cardiomyocytes. Conclusions Based on these results, the 18F-labeled diethylene glycol ester of rhodamine 6G is the most promising potential PET MPI radiopharmaceutical of those that have been evaluated to date, and we are now preparing to carry out first-in-human clinical studies with this compound. PMID:26205075

  1. IV and IP administration of rhodamine in visualization of WBC-BBB interactions in cerebral vessels.

    Science.gov (United States)

    Reichenbach, Zachary Wilmer; Li, Hongbo; Gaughan, John P; Elliott, Melanie; Tuma, Ronald

    2015-10-01

    Epi-illuminescence intravital fluorescence microscopy has been employed to study leukocyte-endothelial interactions in a number of brain pathologies. Historically, dyes such as Rhodamine 6G have been injected intravenously. However, intravenous injections can predispose experimental animals to a multitude of complications and requires a high degree of technical skill. Here, we study the efficacy of injecting Rhodamine 6G into the peritoneum (IP) for the purpose of analyzing leukocyte-endothelial interactions through a cranial window during real time intravital microscopy. After examining the number of rolling and adherent leukocytes through a cranial window, we found no advantage to the intravenous injection (IV). Additionally, we tested blood from both routes of injection by flow cytometry to gain a very precise picture of the two methods. The two routes of administration failed to show any difference in the ability to detect cells. The study supports the notion that IP Rhodamine 6G works as efficaciously as IV and should be considered a viable alternative in experimental design for investigations employing intravital microscopy. Facilitated intravital studies will allow for more exploration into cerebral pathologies and allow for more rapid translation from the laboratory to the patient with less chance of experimental error from failed IV access. © 2015 Wiley Periodicals, Inc.

  2. for aqueous dye lasers

    Indian Academy of Sciences (India)

    2014-02-12

    Feb 12, 2014 ... inclusion complex of RhB with the container molecule cucurbit[7]uril (CB[7]). Keywords. Temperature-dependent fluorescence; Rhodamine B; cucurbit[7]uril; host–guest complex; dye laser. PACS Nos 36.20.kd; 83.60.pq; 87.64.kv. 1. Introduction. Rhodamine B (RhB) is an efficient and photostable laser dye ...

  3. Preparation and flow cytometry of uniform silica-fluorescent dye microspheres.

    Science.gov (United States)

    Bele, Marjan; Siiman, Olavi; Matijević, Egon

    2002-10-15

    Uniform fluorescent silica-dye microspheres have been prepared by coating preformed monodispersed silica particles with silica layers containing rhodamine 6G or acridine orange. The resulting dispersions exhibit intense fluorescent emission between 500 and 600 nm, over a broad excitation wavelength range of 460 to 550 nm, even with exceedingly small amounts of dyes incorporated into the silica particles (10-30 ppm, expressed as weight of dye relative to weight of dry particles). The fluorescent particles can be prepared in micrometer diameters suitable for analyses using flow cytometry with 488-nm laser excitation.

  4. Synthesis of Rhodamines from Fluoresceins Using Pd-Catalyzed C–N Cross-Coupling

    Science.gov (United States)

    2011-01-01

    A unified, convenient, and efficient strategy for the preparation of rhodamines and N,N′-diacylated rhodamines has been developed. Fluorescein ditriflates were found to undergo palladium-catalyzed C–N cross-coupling with amines, amides, carbamates, and other nitrogen nucleophiles to provide direct access to known and novel rhodamine derivatives, including fluorescent dyes, quenchers, and latent fluorophores. PMID:22091952

  5. Optimum design of a multi-stage dye-laser amplifier pumped with Cu-vapor lasers

    International Nuclear Information System (INIS)

    Maeda, Mitsuo; Uchiumi, Michihiro

    1990-01-01

    A numerical simulation code, based on the one-dimensional photon transport equation, was developed and analyzed to evaluate the performances of Rhodamine 6G dye laser amplifiers pumped with Cu-vapor lasers. The upper singlet-state absorption played an important role to determine the efficiency. The simulation code was applied to optimize a multi-stage amplifier system with a pulsed or a CW dye-laser oscillator. The analytical results gave a useful guideline to design a high-power pulsed dye-laser system for atomic uranium enrichment. (author)

  6. Studies on widely tunable ultra-short laser pulses using energy transfer distributed feedback dye laser

    International Nuclear Information System (INIS)

    Ahamed, M.B.; Ramalingam, A.; Palanisamy, P.K.

    2003-01-01

    This paper presents both theoretical and experimental study of the characteristics of Nd: YAG laser pumped energy transfer distributed feedback dye laser (ETDFDL). Using theoretical model proposed, the behavior of ETDFDL such as the characteristics of donor DFDL, the acceptor DFDL, the dependence of their pulse width and output power on donor-acceptor concentrations and pump power are studied for dye mixture Rhodamine 6G and Cresyl Violet in detail. Experimentally using prism-dye cell configuration, the ETDFDL output is obtained and the output energy of DFDL is measured at the emission peaks of donor and acceptor dyes for different pump powers and donor-acceptor concentrations. In addition, the DFDL linewidth measurement has been carried out at the lasing wavelengths of the donor and acceptor dyes using Fabry-Perot etalon and the tunability of DFDL is measured to be in the wavelength range of 545-680 nm

  7. Nanotubular halloysite clay as efficient water filtration system for cationic and anionic dyes removal

    OpenAIRE

    Conference, Nanostruc; Yafei Zhao, Elshad Abdullayev and Yuri Lvov

    2014-01-01

    Halloysite clay has chemical structure similar to kaolinite but it is rolled in tubes with diameter of 50 nm and length of ca. 1000 nm. Halloysite exhibits higher adsorption capacity for both cationic and anionic dyes because it has negative SiO2 outermost and positive Al2O3 inner lumen surface. An adsorption study using cationicRhodamine 6G and anionic Chrome azurol S has shown pproximately two times better dye removal for halloysite as compared to kaolin. Halloysite filters have been effect...

  8. Development of the plastic solid-dye cell for tunable solid-state dye lasers and study on its optical properties

    International Nuclear Information System (INIS)

    Ko, Do Kyeong; Lee, Jong Min; Cha, Byung Heon; Yi, Jong Hoon; Lee, Kang Soo; Kim, Sung Ho; Lim, Gwon

    2000-01-01

    We have fabricated solid-state dyes with Copolex NK-55, which is the base element of plastic lens, and PMMA. We have measured the longevity of solid-state dyes doped in both polymers and found that PMMA has better properties than Coploex NK-55. We have realized the tuning range of 560-620 nm by doping rhodamine 6G and rhodamin B in the manufactured solid-state dye laser oscillators. In the standing-wave cavity we achieved the slop efficiency of 10.8 percent and in the grazing incidence cavity, 1.2 percent. We have constructed a very compact grazing- incidence cavity which is only 6 cm long and the linewidth of the laser was less than 1.5 GHz with 3-ns pulse duration. And we have fabricated disk-type solid-state dye cell and installed it in the cavity in which the dye cell can be translated and rotated with the help of the two steeping motors. By this we could constantly changed the illuminated area of the dye cell and , therefore, were able to achieve long time operation and to use almost the entire region of the solid-state dye cell. (author)

  9. Bright new colours: the history and analysis of fluorescein, eosin, erythrosine, rhodamine and some of their derivatives

    NARCIS (Netherlands)

    de Keijzer, M.; van Bommel, M.R.; Kirby, J.

    2017-01-01

    This paper discusses the history, chemical constitution, production, dyeing processes and properties of the hydroxyphthaleins. Important dyes of this dye class are fluorescein, the eosins, phloxines, erythrosines, rose bengals and rhodamines. These dyes were used for textile dyeing and as painting

  10. Quenching of photoluminescence of Rhodamine 6G molecules on functionalized graphene

    Czech Academy of Sciences Publication Activity Database

    Valeš, Václav; Kovaříček, Petr; Ji, X.; Ling, X.; Kong, J.; Dresselhaus, M. S.; Kalbáč, Martin

    2016-01-01

    Roč. 253, č. 12 (2016), s. 2347-2350 ISSN 0370-1972 Institutional support: RVO:61388955 Keywords : atomic force microscopy * functionalized graphene * photoluminiscence quenching Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.674, year: 2016

  11. Tip Enhanced Raman Spectroscopy of Rhodamine 6G on nanostructured gold substrate

    KAUST Repository

    Moretti, Manola

    2015-05-01

    A new concept based setup for Tip Enhanced Raman Scattering measurement assisted by gold nanostructure is presented, that can provide a platform for gap-mode enhancement of the signal at the single molecule level conjugated with controlled spatial localization of the molecule under investigation and a method to determine the diffraction limit properties of the tip. In essence, this effect is obtained illuminating a gold coated AFM tip which is raster scanned over a nanostructured gold substrate, after chemisorption of a Raman active molecule. We expect that the near-field Raman enhancement would be given by the gap-mode effect of the two facing nano-features. Thanks to the nanostructured substrate, we verify that the resolution of the Raman mapping signal is well below the diffraction limit given by the combination of the optics geometry and the laser wavelength. We show that the gap-mode TERS can generate an estimated field- enhancement (g) of ~20 in localized areas of the sample and we demonstrate the ability to spatially define the molecule position (by Raman mapping) at the tens of nanometers scale. © 2015 Elsevier Ltd.

  12. Tip Enhanced Raman Spectroscopy of Rhodamine 6G on nanostructured gold substrate

    KAUST Repository

    Moretti, Manola; Das, Gobind; Torre, Bruno; Allione, Marco; Di Fabrizio, Enzo M.

    2015-01-01

    A new concept based setup for Tip Enhanced Raman Scattering measurement assisted by gold nanostructure is presented, that can provide a platform for gap-mode enhancement of the signal at the single molecule level conjugated with controlled spatial localization of the molecule under investigation and a method to determine the diffraction limit properties of the tip. In essence, this effect is obtained illuminating a gold coated AFM tip which is raster scanned over a nanostructured gold substrate, after chemisorption of a Raman active molecule. We expect that the near-field Raman enhancement would be given by the gap-mode effect of the two facing nano-features. Thanks to the nanostructured substrate, we verify that the resolution of the Raman mapping signal is well below the diffraction limit given by the combination of the optics geometry and the laser wavelength. We show that the gap-mode TERS can generate an estimated field- enhancement (g) of ~20 in localized areas of the sample and we demonstrate the ability to spatially define the molecule position (by Raman mapping) at the tens of nanometers scale. © 2015 Elsevier Ltd.

  13. Localization of mitochondria in living cells with rhodamine 123.

    Science.gov (United States)

    Johnson, L V; Walsh, M L; Chen, L B

    1980-01-01

    The laser dye rhodamine 123 is shown to be a specific probe for the localization of mitochondria in living cells. By virtue of its selectivity for mitochondria and its fluorescent properties, the detectability of mitochondria stained with rhodamine 123 is significantly improved over that provided by conventional light microscopic techniques. With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment. Images PMID:6965798

  14. Synthesis of a Far-Red Photoactivatable Silicon-Containing Rhodamine for Super-Resolution Microscopy.

    Science.gov (United States)

    Grimm, Jonathan B; Klein, Teresa; Kopek, Benjamin G; Shtengel, Gleb; Hess, Harald F; Sauer, Markus; Lavis, Luke D

    2016-01-26

    The rhodamine system is a flexible framework for building small-molecule fluorescent probes. Changing N-substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si-containing analogue of Q-rhodamine. This probe is the first example of a "caged" Si-rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red-shifted to allow multicolor imaging. The dye is a useful label for super-resolution imaging and constitutes a new scaffold for far-red fluorogenic molecules. © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

  15. Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy

    Science.gov (United States)

    Grimm, Jonathan B.; Klein, Teresa; Kopek, Benjamin G.; Shtengel, Gleb; Hess, Harald F.; Sauer, Markus

    2015-01-01

    Abstract The rhodamine system is a flexible framework for building small‐molecule fluorescent probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si‐containing analogue of Q‐rhodamine. This probe is the first example of a “caged” Si‐rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red‐shifted to allow multicolor imaging. The dye is a useful label for super‐resolution imaging and constitutes a new scaffold for far‐red fluorogenic molecules. PMID:26661345

  16. IDENTIFIKASI RHODAMIN B PADA SAUS TOMAT YANG BEREDAR DI PASAR PAGI SAMARINDA

    Directory of Open Access Journals (Sweden)

    Eka Siswanto Syamsul

    2018-03-01

    Full Text Available Rhodamin B is a green powder synthetic dye used as a textile dye and is often used to color a food product, especially tomato sauce. Tomato sauce sampled in Samarinda Morning Market for 5 samples. This research used color reaction test and KLT method. The result of the research using color reaction test showed that the sample did not contain rhodamine synthetic dye B. The result of identification with thin layer chromatography obtained Rf value of A sample 0,46, sample B 0,46, sample C 0,4, sample D 0,5 and sample E 0.78, the value of Rf standard rhodamin B 0.8. From the value of Rf, the sample E contains rhodamine B because the color of the sample spots and rhodamin B are the same pink and the difference of Rf value is in the range ± 0.02 of the standard Rf.

  17. Tunable lasers in isotope separation: a colorful view of a dye chemist

    International Nuclear Information System (INIS)

    Hammond, P.R.

    1977-01-01

    Some of the problems to be encountered in the possible large scale use of dye lasers in an isotope separation plant are discussed.The effect of laser dye deterioration on performance is examined algebraically in terms of disappearance of dye molecules and the appearance of a new, single chemical product having absorption in the fluorescence band for a single pass through a transversely pumped amplifier. Loss of output, defined as ''quantum yield of laser deterioration'', Q/sub L/, is related to the true quantum yield of molecular destruction of the dye Q/sub M/, and other known parameters. 6-Diethylamino 3-keto fluoran, an example of an oxygen tricyclic merocyanine, is described. It was first reported in the pre-1900 German literature under the name of Chromogen Red B and it is an ineffective lasing dye on account of low fluorescence quantum yield. The techniques for measurement and the excited state absorption cross-sections are reported for the dyes rhodamine 6G fluoroborate in alcohol, rhodamine B basic solution in trifluoroethanol and kiton red S in trifluoroethanol

  18. Nanotubular Halloysite Clay as Efficient Water Filtration System for Removal of Cationic and Anionic Dyes

    International Nuclear Information System (INIS)

    Zhao, Yafei; Abdullayev, Elshad; Lvov, Yuri

    2014-01-01

    Halloysite nanotubes, chemically similar to kaolinite, are formed by rolling of kaolinite layers in tubes with diameter of 50 nm and length of ca. 1 μm. Halloysite has negative SiO 2 outermost and positive Al 2 O 3 inner lumen surface, which enables it to be used as potential absorbent for both cationic and anionic dyes due to the efficient bivalent adsorbancy. An adsorption study using cationic Rhodamine 6G and anionic Chrome azurol S has shown approximately two times better dye removal for halloysite as compared to kaolinite. Halloysite filters have been effectively regenerated up to 50 times by burning the adsorbed dyes. Overall removal efficiency of anionic Chrome azurol S exceeded 99.9% for 5th regeneration cycle of halloysite. Chrome azurol S adsorption capacity decreases with the increase of ionic strength, temperature and pH. For cationic Rhodamine 6G, higher ionic strength, temperature and initial solution concentration were favorable to enhanced adsorption with optimal pH 8. These results indicate a potential to utilize halloysite for the removal of ionic dyes from environmental waters

  19. Influence of silver nanoparticles on relaxation processes and efficiency of dipole – dipole energy transfer between dye molecules in polymethylmethacrylate films

    Energy Technology Data Exchange (ETDEWEB)

    Bryukhanov, V V; Borkunov, R Yu; Tsarkov, M V [Immanuel Kant Baltic Federal University, Kaliningrad (Russian Federation); Konstantinova, E I; Slezhkin, V A [Kaliningrad State Technical University, Kaliningrad (Russian Federation)

    2015-10-31

    The fluorescence and phosphorescence of dyes in thin polymethylmethacrylate (PMMA) films in the presence of ablated silver nanoparticles has been investigated in a wide temperature range by methods of femtosecond and picosecond laser photoexcitation. The fluorescence and phosphorescence times, as well as spectral and kinetic characteristics of rhodamine 6G (R6G) molecules in PMMA films are measured in a temperature range of 80 – 330 K. The temperature quenching activation energy of the fluorescence of R6G molecules in the presence of ablated silver nanoparticles is found. The vibrational relaxation rate of R6G in PMMA films is estimated, the efficiency of the dipole – dipole electron energy transfer between R6G and brilliant green molecules (enhanced by plasmonic interaction with ablated silver nanoparticles) is analysed, and the constants of this energy transfer are determined. (nanophotonics)

  20. Influence of silver nanoparticles on relaxation processes and efficiency of dipole – dipole energy transfer between dye molecules in polymethylmethacrylate films

    International Nuclear Information System (INIS)

    Bryukhanov, V V; Borkunov, R Yu; Tsarkov, M V; Konstantinova, E I; Slezhkin, V A

    2015-01-01

    The fluorescence and phosphorescence of dyes in thin polymethylmethacrylate (PMMA) films in the presence of ablated silver nanoparticles has been investigated in a wide temperature range by methods of femtosecond and picosecond laser photoexcitation. The fluorescence and phosphorescence times, as well as spectral and kinetic characteristics of rhodamine 6G (R6G) molecules in PMMA films are measured in a temperature range of 80 – 330 K. The temperature quenching activation energy of the fluorescence of R6G molecules in the presence of ablated silver nanoparticles is found. The vibrational relaxation rate of R6G in PMMA films is estimated, the efficiency of the dipole – dipole electron energy transfer between R6G and brilliant green molecules (enhanced by plasmonic interaction with ablated silver nanoparticles) is analysed, and the constants of this energy transfer are determined. (nanophotonics)

  1. Efficient Cycloaddition Reaction of Carbon Dioxide with Epoxide by Rhodamine Based Catalyst Under 1 atm Pressure

    Energy Technology Data Exchange (ETDEWEB)

    Gong, Qing; Luo, Huadong; Cao, Di; Zhang, Haibo; Wang, Wenjing; Zhou, Xiaohai [Wuhan University, Wuhan (China)

    2012-06-15

    Rhodamine B (RhB) and rhodamine 6G (Rh6G) were employed as catalysts for the synthesis of cyclic carbonate from carbon dioxide and epoxide. It turned out that the catalytic activity of Rh6G was nearly 29 times higher than that of RhB at 1 atm pressure, 90 .deg. C. Furthermore, the catalytic efficiency of RhB and Rh6G was greatly enhanced with triethylamine as co-catalyst. Under the optimized conditions, the best isolated yield (93%) of cyclic carbonate was achieved without organic solvent and metal component

  2. Coulombic interactions on the deposition and rotational mobility distributions of dyes in polyelectrolyte multilayer thin films.

    Science.gov (United States)

    Li, Ye; Yip, Wai Tak

    2004-12-07

    We employed negatively charged fluorescein (FL), positively charged rhodamine 6G (R6G), and neutral Nile Red (NR) as molecular probes to investigate the influence of Coulombic interaction on their deposition into and rotational mobility inside polyelectrolyte multilayer (PEM) films. The entrapment efficiency of the dyes reveals that while Coulombic repulsion has little effect on dye deposition, Coulombic attraction can dramatically enhance the loading efficiency of dyes into a PEM film. By monitoring the emission polarization of single dye molecules in polyethylenimine (PEI) films, the percentages of mobile R6G, NR, and FL were determined to be 87 +/- 4%, 76 +/- 5%, and 68 +/- 3%, respectively. These mobility distributions suggest that cationic R6G enjoys the highest degree of rotational freedom, whereas anionic FL shows the least mobility because of Coulombic attraction toward cationic PEI. Regardless of charges, this high percentage of mobile molecules is in stark contrast to the 5-40% probe mobility reported from spun-cast polymer films, indicating that our PEI films contain more free volume and display richer polymer dynamics. These observations demonstrate the potential of using isolated fluorescent probes to interrogate the internal structure of a PEM film at a microscopic level.

  3. Investigation on ionic states of 1,2-Dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC) using organic laser dyes: A FRET study

    Energy Technology Data Exchange (ETDEWEB)

    Roy, Arpan Datta; Saha, Jaba; Dey, D.; Bhattacharjee, D.; Hussain, Syed Arshad, E-mail: sa_h153@hotmail.com

    2017-05-15

    Fluorescence Resonance Energy Transfer (FRET) between two organic dyes Fluorescein and Rhodamine 6G were successfully investigated in aqueous solution in presence and absence of 1,2-Dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC) at different pH. Spectroscopic studies suggest that both the dyes were present mainly as monomer in solution. FRET occurred from Fluorescein to Rhodamine 6G in solutions. Energy transfer efficiency increases in presence of DPPC and the maximum efficiency was 59.3% when the concentration of DPPC was 1.4×10{sup −4} M at ambient condition. pH plays a crucial role in this investigation as the energy transfer efficiency was found to change in presence of DPPC at different pH. It has been demonstrated that with proper calibration it is possible to use the present system under investigation to realize various ionic states of DPPC by observing the change in FRET efficiency between these two dyes. - Graphical abstract: Electrostatic interaction between anionic Flu and cationic R6G molecules in presence and absence of DPPC at different pH. Here pH of DPPC was changed, not the pH of individual dyes.

  4. Charge transfer in graphene oxide-dye system for photonic applications

    International Nuclear Information System (INIS)

    Bongu, Sudhakara Reddy; Bisht, Prem B.; Thu, Tran V.; Sandhu, Adarsh

    2014-01-01

    The fluorescence of a standard dye Rhodamine 6G (R6G) in solution decreases on addition of reduced graphene oxide (rGO). The absorption spectra and lifetime measurements confirm that no excited-state but a ground-state complex formation is responsible for this effect. For silver decorated rGO (Ag-rGO), the quenching efficiency and ground state complex formation process is small. Z-scan measurements have been done to study the optical nonlinearity at 532 nm under ps time scale. Remarkable reduction in the saturable absorption (SA) effect of R6G indicates no nonlinear contribution from the ground state complex. The results have been explained with varying charge transfer rates and non-fluorescence nature of the complex

  5. Laser-induced incandescence of suspended particles as a source of excitation of dye luminescence

    CERN Document Server

    Zelensky, S

    2003-01-01

    The interaction of pulsed YAG-Nd sup 3 sup + laser radiation with submicron light-absorbing particles suspended in an aqueous solution of Rhodamine 6G is investigated experimentally. The experiments demonstrate that the laser-induced incandescence of suspended particles excites the luminescence of the dissolved dye molecules. The mechanism of the luminescence excitation consists in the reabsorption of the thermal radiation within the volume of the sample cell. On the ground of this mechanism of excitation, a method of measurement of the luminescence quantum yield is proposed and realized. The method requires the knowledge of the geometrical parameters of the cell and does not require the use of reference samples.

  6. Rhodamine 800 as reference substance for fluorescence quantum yield measurements in deep red emission range

    Energy Technology Data Exchange (ETDEWEB)

    Alessi, A., E-mail: andrea.alessi@eni.com [Centro Ricerche per le Energie non Convenzionali, Istituto eni Donegani, e.n.i. S.p.A., Via G. Fauser 4, 28100 Novara (Italy); Salvalaggio, M. [Centro Ricerche per le Energie non Convenzionali, Istituto eni Donegani, e.n.i. S.p.A., Via G. Fauser 4, 28100 Novara (Italy); Ruzzon, G. [HORIBA Jobin Yvon Srl, Via Cesare Pavese 35/AB, 20090 Opera Milano (Italy)

    2013-02-15

    The determination of fluorescence quantum yields ({Phi}{sub f}) of deep red dyes emitting at 635-900 nm is difficult due to lack of suitable standards. In this work, we propose a commercial dye, rhodamine 800 (Rho800), as reference standard which belongs to the family of xanthenes. The quantum yield of rhodamine 800 in absolute ethanol has been studied using a relative method with cresyl violet (CV) and rhodamine 101 (Rho101) as references, and an absolute fluorometric method by integrating sphere measurements. - Highlights: Black-Right-Pointing-Pointer A red emitting dye Rhodamine 800 was electronic spectroscopy characterized. Black-Right-Pointing-Pointer Its fluorescence quantum yield was studied using a relative and an absolute method. Black-Right-Pointing-Pointer The values found are greater than the values currently known in the literature.

  7. Fabrication of a large-area, flexible micro-pyramid PET film SERS substrate and its application in the detection of dye in herbal tea

    Science.gov (United States)

    Liu, Xi; Huang, Meizhen; Chen, Jie; Kong, Lili; Wang, Keihui

    2018-05-01

    A simple method, based on a roll-to-roll ultraviolet micro-pyramid imprinting technique and a nanoparticle self-assembling process in aqueous solution, to fabricate a large-area, flexible surface-enhanced Raman scattering (SERS) polyethylene glycol terephthalate substrate is proposed. The SERS substrate is demonstrated to be of high sensitivity. The detection concentration of Rhodamine 6G (R6G) measured by a portable Raman spectrometer is down to 10-9 mol l-1. The relative standard deviation values of different spots and different substrates are less than 13%. In addition, the feasibility for rapid detection of dye in herbal tea based on this SERS substrate and a portable Raman spectrometer is investigated. Three industrial dyes are employed to simulate the dyeing process. It is presented that R6G of 4.8× {{10}-7} g ml-1, malachite green of 10-6 g ml-1 and Auramine O of 10-6 g ml-1 in herbal tea could be detected rapidly. The experimental results imply that this method could be potentially applied in the field of dyed herbal tea detection.

  8. Preparation of Rhodamine B Fluorescent Poly(methacrylic acid) Coated Gelatin Nanoparticles

    OpenAIRE

    Gan, Zhenhai; Ju, Jianhui; Zhang, Ting; Wu, Daocheng

    2011-01-01

    Poly(methacrylic acid) (PMAA)-coated gelatin nanoparticles encapsulated with fluorescent dye rhodamine B were prepared by the coacervation method with the aim to retard the release of rhodamine B from the gelatin matrix. With sodium sulfate as coacervation reagent for gelatin, a kind of biopolymer with excellent biocompatibility, the formed gelatin nanoparticles were cross-linked by formaldehyde followed by the polymerization of methacrylic acid coating. The fluorescent poly(methacrylic acid)...

  9. Influence of the UV Radiation on Rhodamine WT Fluorescence in Water Samples

    OpenAIRE

    , L. Kola; , P. Lazo

    2016-01-01

    The şuorescence ability of Rhodamine WT enables its using as artiŞcial tracer in the water system studies. The problem is dealt with in relation to applying Rhodamine WT (RhWT) to trace and determine water movements within the karstic system and underground waters. Rhodamine WT has been used as an artiŞcial tracer for the Şrst times in our country on Mali me Gropa system study (2002). UV radiation may induce photochemical decomposition of the dye which can cause large measurement errors on me...

  10. Single-molecule studies of oligomer extraction and uptake of dyes in poly(dimethylsiloxane) films.

    Science.gov (United States)

    Lange, Jeffrey J; Collinson, Maryanne M; Culbertson, Christopher T; Higgins, Daniel A

    2009-12-15

    Single-molecule microscopic methods were used to probe the uptake, mobility, and entrapment of dye molecules in cured poly(dimethylsiloxane) (PDMS) films as a function of oligomer extraction. The results are relevant to the use of PDMS in microfluidic separations, pervaporation, solid-phase microextraction, and nanofiltration. PDMS films were prepared by spin-casting dilute solutions of Sylgard 184 onto glass coverslips, yielding approximately 1.4 microm thick films after curing. Residual oligomers were subsequently extracted from the films by "spin extraction". In this procedure, 200 microL aliquots of isopropyl alcohol were repeatedly dropped onto the film surface and spun off at 2000 rpm. Samples extracted 5, 10, 20, and 40 times were investigated. Dye molecules were loaded into these films by spin-casting nanomolar dye solutions onto the films. Both neutral perylene diimide (N,N'-bis(butoxypropyl)perylene-3,4,9,10-tetracarboxylic diimide) and cationic rhodamine 6G (R6G) dyes were employed. The films were imaged by confocal fluorescence microscopy. The images obtained depict nonzero populations of fixed and mobile molecules in all films. Cross-correlation methods were used to quantitatively determine the population of fixed molecules in a given region, while a Bayesian burst analysis was used to obtain the total population of molecules. The results show that the total amount of dye loaded increases with increased oligomer extraction, while the relative populations of fixed and mobile molecules decrease and increase, respectively. Bulk R6G data also show greater dye loading with increased oligomer extraction.

  11. Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining

    Science.gov (United States)

    Zaitsev, Sergei Yu.; Shaposhnikov, Mikhail N.; Solovyeva, Daria O.; Solovyeva, Valeria V.; Rizvanov, Albert A.

    2014-01-01

    Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (“Chitosan-PFD”) and histone H1 (“Histone H1.3-PFD”). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (“caged” dyes) for microscopic probing of biological objects. Thus, the synthesized “Chitosan-PFD” and “Histone H1-PFD” have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy. PMID:25383365

  12. Recent advance to 3 × 10(-5) rad near diffraction-limited beam divergence of dye laser with transverse-discharge flash-lamp pumping.

    Science.gov (United States)

    Trusov, K K

    1994-02-20

    A new experimental setup of a Rhodamine 6G dye laser with a transverse-discharge flash-lamp-pumping system is presented. It differs from a previous setup [Sov. J. Quantum Electron. 16, 468-471 (1989)] in that it has a larger laser beam aperture (32 mm) and higher pumping energy (1 kJ), which made it possible to test the scalability and reach near diffraction-limited laser beam divergence of 3 × 10(-5) rad FWHM at beam energy 1.4 J. The effect of spectral dispersion in the active medium and of other optical elements on the beam divergence is also discussed.

  13. Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent

    International Nuclear Information System (INIS)

    Heinrich, Tobias K.; Gottumukkala, Vijay; Snay, Erin; Dunning, Patricia; Fahey, Frederic H.; Ted Treves, S.; Packard, Alan B.

    2010-01-01

    There is considerable interest in developing an 18 F-labeled PET myocardial perfusion agent. Rhodamine dyes share several properties with 99m Tc-MIBI, the most commonly used single-photon myocardial perfusion agent, suggesting that an 18 F-labeled rhodamine dye might prove useful for this application. In addition to being lipophilic cations, like 99m Tc-MIBI, rhodamine dyes are known to accumulate in the myocardium and are substrates for Pgp, the protein implicated in MDR1 multidrug resistance. As the first step in determining whether 18 F-labeled rhodamines might be useful as myocardial perfusion agents for PET, our objective was to develop synthetic methods for preparing the 18 F-labeled compounds so that they could be evaluated in vivo. Rhodamine B was chosen as the prototype compound for development of the synthesis because the ethyl substituents on the amine moieties of rhodamine B protect them from side reactions, thus eliminating the need to include (and subsequently remove) protecting groups. The 2'-[ 18 F]fluoroethyl ester of rhodamine B was synthesized by heating rhodamine B lactone with [ 18 F]fluoroethyltosylate in acetonitrile at 165 deg. C for 30 min using [ 18 F]fluoroethyl tosylate, which was prepared by the reaction of ethyleneglycol ditosylate with Kryptofix 2.2.2, K 2 CO 3 , and [ 18 F]NaF in acetonitrile for 10 min at 90 deg. C. The product was purified by semi-preparative HPLC to produce the 2'-[ 18 F]fluoroethylester in >97% radiochemical purity with a specific activity of 1.3 GBq/μmol, an isolated decay corrected yield of 35%, and a total synthesis time of 90 min.

  14. Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent

    Science.gov (United States)

    Heinrich, Tobias K.; Gottumukkala, Vijay; Snay, Erin; Dunning, Patricia; Fahey, Frederic H; Treves, S. Ted; Packard, Alan B.

    2009-01-01

    There is considerable interest in developing an 18F-labeled PET myocardial perfusion agent. Rhodamine dyes share several properties with 99mTc-MIBI, the most commonly used single-photon myocardial perfusion agent, suggesting that an 18F-labeled rhodamine dye might prove useful for this application. In addition to being lipophilic cations, like 99mTc-MIBI, rhodamine dyes are known to accumulate in the myocardium and are substrates for Pgp, the protein implicated in MDR1 multidrug resistance. As the first step in determining whether 18F-labeled rhodamines might be useful as myocardial perfusion agents for PET, our objective was to develop synthetic methods for preparing the 18F-labeled compounds so that they could be evaluated in vivo. Rhodamine B was chosen as the prototype compound for development of the synthesis because the ethyl substituents on the amine moieties of rhodamine B protect them from side reactions, thus eliminating the need to include (and subsequently remove) protecting groups. The 2′-[18F]fluoroethyl ester of rhodamine B was synthesized by heating rhodamine B lactone with [18F]fluoroethyltosylate in acetonitrile at 165°C for 30 min.using [18F]fluoroethyl tosylate, which was prepared by the reaction of ethyleneglycol ditosylate with Kryptofix 2.2.2, K2CO3, and [18F]NaF in acetonitrile for 10 min. at 90°C. The product was purified by semi-preparative HPLC to produce the 2′-[18F]-fluoroethylester in >97% radiochemical purity with a specific activity of 1.3 GBq/μmol, an isolated decay corrected yield of 35%, and a total synthesis time of 90 min. PMID:19783150

  15. Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Heinrich, Tobias K.; Gottumukkala, Vijay [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States); Snay, Erin; Dunning, Patricia [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Fahey, Frederic H.; Ted Treves, S. [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States); Packard, Alan B. [Division of Nuclear Medicine, Department of Radiology, Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States)], E-mail: alan.packard@childrens.harvard.edu

    2010-01-15

    There is considerable interest in developing an {sup 18}F-labeled PET myocardial perfusion agent. Rhodamine dyes share several properties with {sup 99m}Tc-MIBI, the most commonly used single-photon myocardial perfusion agent, suggesting that an {sup 18}F-labeled rhodamine dye might prove useful for this application. In addition to being lipophilic cations, like {sup 99m}Tc-MIBI, rhodamine dyes are known to accumulate in the myocardium and are substrates for Pgp, the protein implicated in MDR1 multidrug resistance. As the first step in determining whether {sup 18}F-labeled rhodamines might be useful as myocardial perfusion agents for PET, our objective was to develop synthetic methods for preparing the {sup 18}F-labeled compounds so that they could be evaluated in vivo. Rhodamine B was chosen as the prototype compound for development of the synthesis because the ethyl substituents on the amine moieties of rhodamine B protect them from side reactions, thus eliminating the need to include (and subsequently remove) protecting groups. The 2'-[{sup 18}F]fluoroethyl ester of rhodamine B was synthesized by heating rhodamine B lactone with [{sup 18}F]fluoroethyltosylate in acetonitrile at 165 deg. C for 30 min using [{sup 18}F]fluoroethyl tosylate, which was prepared by the reaction of ethyleneglycol ditosylate with Kryptofix 2.2.2, K{sub 2}CO{sub 3}, and [{sup 18}F]NaF in acetonitrile for 10 min at 90 deg. C. The product was purified by semi-preparative HPLC to produce the 2'-[{sup 18}F]fluoroethylester in >97% radiochemical purity with a specific activity of 1.3 GBq/{mu}mol, an isolated decay corrected yield of 35%, and a total synthesis time of 90 min.

  16. Effects of single and double bonds in linkers on colorimetric and fluorescent sensing properties of polyving akohol grafting rhodamine hydrazides.

    Science.gov (United States)

    Geng, Tong-Mou; Wang, Xie; Wang, Zhu-Qing; Chen, Tai-Jie; Zhu, Hai; Wang, Yu

    2015-03-01

    Two rhodamine derivatives, N-mono-maleic acid amide-N'-rhodamine B hydrazide (MRBH) and N-mono-succinic acid amide-N'-rhodamine 6G hydrazide (SR6GH), were synthesized by amidation with maleic anhydride (MAH), succinic anhydride (SAH) and rhodamine B hydrazide, rhodamine 6G hydrazide, which were identified by FTIR, (1)H NMR and elemental analysis. Two water-soluble fluorescent materials (PVA-MRBH and PVA-SR6GH) were prepared via esterification reaction with N-mono-maleic acyl chloride amide-N'-rhodamine B hydrazide (MRBHCl) or N-mono-maleic acyl chloride amide-N'-rhodamine 6G hydrazide (SR6GHCl) and poly(vinyl alcohol) (PVA) in DMSO solution. The sensing behaviors of PVA-MRBH and PVA-SR6GH were explored by recording the fluorescence spectra in completely aqueous solution. Upon the addition of Cu(2+) and Fe(3+) ions to the aqueous solution of PVA-MRBH, visual color change from rose pink to amaranth and orange for Cu(2+) and Fe(3+) ions, respectively, and fluorescence quenching were observed. Titration of Cu(2+), Fe(3+), Cr(3+) or Hg(2+) into the aqueous solution of PVA-SR6GH, although they induced fluorescence enhancement, only Fe(3+) made the color changing from colorless to yellow. Moreover, other metal ions did not induce obvious changes to color and the fluorescence spectra.

  17. Analisa Kandungan Rhodamin B dan Formalin pada Gula Merah Serta Pengetahuan dan Sikap Pedagang di Pasar Tradisional Kecamatan Medan Baru Tahun 2013

    OpenAIRE

    Sihombing, Emma Sari Yanti

    2014-01-01

    Rhodamine B and formaldehyde is colouration substance and preservative substance that prohibits to be utilized in food product. But there are still traders using rhodamine B and formaldehyde in food products and beverages. Brown sugar is one of the food ingredients that are often encountered in daily life. The purpose of this research is to determine the rhodamine B dye and preservative formaldehyde in brown sugar in the traditional market sub-district Medan Baru in 2013 and determine the ...

  18. Analisa Kandungan Rhodamin B dan Formalin pada Gula Merah Serta Pengetahuan dan Sikap Pedagang di Pasar Tradisional Kecamatan Medan Baru Tahun 2013

    OpenAIRE

    sihombing, emma; hasan, wirsal; marsaulina, irnawati

    2014-01-01

    Rhodamine B and formaldehyde is colouration substance and preservative substance that prohibits to be utilized in food product. But there are still traders using rhodamine B and formaldehyde in food products and beverages. Brown sugar is one of the food ingredients that are often encountered in daily life. The purpose of this research is to determine the rhodamine B dye and preservative formaldehyde in brown sugar in the traditional market sub-district Medan Baru in 2013 and determine the lev...

  19. ANALISIS ZAT PEWARNA RHODAMIN B PADA SAUS CABAI YANG BEREDAR DI KAMPUS UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

    Directory of Open Access Journals (Sweden)

    Debby Chrislia

    2017-01-01

    Full Text Available Chili sauce is one of the most popular food product because it was used as food complement. Generally in processing manufacture chili sauce added coloring substance, so product colour interesting. Nowadays more and more food manufacturers use dye that have been banned use. Rhodamine B dye in the form of a crystalline powder green or reddish purple, odorless and dissolves easily in bright red solution berfluoresan as textile dyes. Rhodamin b is still no food products were found to countain rhodamine B dye such as crackers, sauces, ice and other pastries. Rhodamin B is a synthetic dye that was banned for use in foods and is certified as a hazardous material according to Minister of Healthy of Indonesian Republic No. 722/Menkes/Per/IX/1998. Rhodamin B is carcinogenic, in the long term use can cause liver damage, kidney inflammation, and cancer. The kind of research this used a quantitative approach with the experimental methods laboratory. The purpose of this study was to determine whether there rhodamin B in the test samples using thin layer chromatography (TLC. The result shows that 7 examined samples doesn’t contain rhodamin B and and shows f count smaller than f table (F count< F table (3,61< 3,71. Synthetic dyes are contained in most of the analyzed samples are dyes that allow its use for food by the Regulation of the Minister of Health of Indonesia such as ponceau 4R and amarant and Contribution of research given by researchers of worksheets students and implementation plan learning

  20. New fluorinated rhodamines for optical microscopy and nanoscopy.

    Science.gov (United States)

    Mitronova, Gyuzel Yu; Belov, Vladimir N; Bossi, Mariano L; Wurm, Christian A; Meyer, Lars; Medda, Rebecca; Moneron, Gael; Bretschneider, Stefan; Eggeling, Christian; Jakobs, Stefan; Hell, Stefan W

    2010-04-19

    New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to undergo sulfonation of the xanthene fragment at the positions 4' and 5'. Two fluorine atoms were introduced into the positions 2' and 7' of the 3',6'-diaminoxanthene fragment in compounds 1 a-d, 1 i-l and 1 m-r. The new rhodamine dyes may be excited with λ=488 or 514 nm light; most of them emit light at λ=512-554 nm (compounds 1 q and 1r at λ=576 and 589 nm in methanol, respectively) and have high fluorescence quantum yields in solution (up to 98 %), relatively long excited-state lifetimes (>3 ns) and are resistant against photobleaching, especially at high laser intensities, as is usually applied in confocal microscopy. Sulfonation of the xanthene fragment with 30 % SO3 in H2SO4 is compatible with the secondary amide bond (rhodamine-CON(Me)CH2CH2COOH) formed with MeNHCH2CH2COOCH3 to providing the sterically unhindered carboxylic group required for further (bio)conjugation reactions. After creating the amino reactive sites, the modified derivatives may be used as fluorescent markers and labels for (bio)molecules in optical microscopy and nanoscopy with very-high light intensities. Further, the new rhodamine dyes are able to pass the plasma membrane of living cells, introducing them as potential labels for recent live-cell-tag approaches. We exemplify the excellent performance of the fluorinated rhodamines in optical microscopy by fluorescence correlation spectroscopy (FCS) and stimulated emission depletion (STED) nanoscopy experiments. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Dye sensitization of antimony-doped CdS photoelectrochemical solar cell

    Energy Technology Data Exchange (ETDEWEB)

    El Zayat, M.Y.; Saed, A.O.; El-Dessouki, M.S. [Department of Physics, Faculty of Science, Cairo University, Giza (Egypt)

    2002-01-31

    Sb-doped CdS single crystal was used as a photoanode to fabricate a photoelectrochemical solar (PECS) cell. The three organic dyes; eosin, thymol blue and rhodamin 6G were used as sensitizers in (PECS) cell. In the absence of the dye, the results showed that with Sb-doped CdS single crystal electrode, a higher power conversion efficiency 9.27% has been achieved compared to 5.7-7.4% for pure crystal. Application of the dye in PECS cell increases the efficiency to about 13%. The efficiency reaches its maximum value when the dye concentration is (2.5x10{sup -5})M, sufficient to cover the surface of the semiconductor electrode with a continuous monolayer of the dye. Exceeding this value resulted in a gradual decrease of the efficiency from its maximum value. Mott-Schottky plots gave a doping density of 3.14x10{sup 17}cm{sup -3} and a space charge width of 4.95x10{sup -6}cm for the sample used. A flat-band potential equal to -0.84V, independent of both frequency and pH, was also predicted. Cyclic voltammetry (c.v.) measurements showed an anodic current peak at 0.4V vs. SCE. The disappearance of this peak after excess addition of the reducing agent Na{sub 2}S, indicates that this peak is due to the PEC corrosion of the semiconductor electrode.

  2. PENURUNAN KADAR RHODAMIN B DALAM AIR LIMBAH DENGAN BIOFILTRASI SISTEM TANAMAN

    Directory of Open Access Journals (Sweden)

    K. Yogi Purnamawati

    2016-01-01

    Full Text Available The textile industry is growing rapidly and as the result it’s producing waste that can harm the environment. One of which is rhodamine B. Rhodamine B is a synthetics dyes that have a form crystalline which an organic base containing amino groups, so it is difficult to degrade naturally by microorganism. Biofiltration system method is one of many ways in handling wastewater. Layered filtration unit of sand and rocks combine with the adsorption of plant and decomposition by microorganisms in rhizosphere so that wastewater can be reused. The aim of this study determined effectiveness and capacity of biofiltration system vegetation in reducing concentrate of rhodamine-B, total dissolved solid (TDS, total suspended solid (TSS and the pH in wastewater. The result showed that biofiltration effectiveness in reducing rhodamine B, TDS and TSS concetrate were 51,70%; 47,60%; 50,44% while decreasing and stabilization of pH obtained at 30 hours treatment time with pH value is 7,5. Capacity of biofiltration system vegetation with volume 0,06 m3 can reduced rhodamine B, TDS and TSS by 0,2256 ppm; 278,0237 ppm and 9,4978 ppm respectively, while the optimum detention time of wastewater in the biosystem for reducing rhodamine B was 30 hours and for TSS and TDS was 36 hours. It can be concluded that biofiltration system vegetation was able to reduce rhodamine B, TDS, TSS and pH of wastewater

  3. On different photodecomposition behaviors of rhodamine B on laponite and montmorillonite clay under visible light irradiation

    KAUST Repository

    Wang, Peng

    2013-12-11

    In this study, laponite and montmorillonite clays were found to be able to decompose rhodamine B upon visible light irradiation (λ>420nm). Very interestingly, it was found that rhodamine B on laponite underwent a stepwise N-deethylation and its decomposition was terminated once rhodamine 110, as a decomposition product, was formed, whereas the same phenomenon was not observed for rhodamine B on montmorillonite, whose decomposition involved chromophore destruction. Mechanistic study revealed that the different photodecomposition behaviors of rhodamine B on laponite and montmorillonite were attributed to the oxidation by different reactive oxygen species, with laponite involving HO2/O2- while montmorillonite involving OH. It was also found that the degradation pathway of rhodamine B on laponite switched from N-deethylation to chromophore destruction when solution pH was changed from 7.0 to 3.0, which was attributed to a much higher fraction of HO2 relative to O2- under pH 3.0 than under pH 7.0. Based on the results, a mechanism of rhodamine dye decomposition on clay under visible light was proposed, involving the clay as an electron acceptor, electron relay between the adsorbed dye molecules and oxygen molecules, and subsequent reactions between the generated dye radical cations and different reactive oxygen species. The results of this study shed light on how to best utilize visible light for organic pollutant degradation on clays within engineered treatment systems as well as on many of naturally occurring pollutant degradation processes in soils and air involving clay. © 2013 King Saud University.

  4. Rhodamine-Injected Eggs to Photographically Identify Small Nest-Predators

    Science.gov (United States)

    Thomas J. Maier; Richard M. DeGraaf

    2000-01-01

    Photographs that clearly disclose avian-nest predators are difficult to obtain, particularly when predators are small and exhibit subtle depredatory behavior. We exposed House Sparrow (Passer domesticus) eggs injected with Rhodamine B dye in camera-monitored ground nests for 12-d periods at 76 sites within mixed-hardwood forest stands in central...

  5. Influence of Ti–O–Si hetero-linkages in the photocatalytic degradation of Rhodamine B

    NARCIS (Netherlands)

    Rasalingam, S; Kibombo, H.S.; Wu, C.M.; Budhi, S.; Peng, R.; Baltrusaitis, Jonas; Koodali, R.T.

    2013-01-01

    The influence of Ti–O–Si hetero-linkages in the degradation of Rhodamine B (RhB) dye over TiO2–SiO2 xerogels is exemplified by XPS analysis. We demonstrate a relationship between the percentage surface content of Ti–O–Si and the rate of photocatalytic degradation of RhB. Our detailed surface

  6. DETEKSI KANDUNGAN RHODAMIN B PADA SAUS SERTA CEMARAN BORAKS DAN BAKTERI SALMONELLA SP. PADACILOK KELILING SALATIGA

    Directory of Open Access Journals (Sweden)

    Ardhikajaya Wahyu Prasetya

    2017-01-01

    Full Text Available ABSTRACT Borax and rhodamine B are preservative and dye banned to be used in food products. However, some traders still use borax and rhodamine B to their food and drink products. Cilok is a kind of food liked by schoolchildren and categorized as street vendor food (PKL. Most of food sold by street vendor has not touched by strict control from BPOM, so its quality is not good. Pentol cilok is a food made by starch. It tastes delicious and chewy. Furthermore, it is liked by schoolchildren. Cilok is also served by adding the sauce in order to make it tastier. It is common to add dye in that sauce and the dye is not natural dye nor food coloring. In the cilok, the contamination of microbe especially Salmonella sp. is influenced by unhygienic process. This research aims to find out borax and Salmonella sp. contamination and also the existence of rhodamine B on the cilok sauce. The methods used in this research are qualitative, quantitative, and the detection of Salmonella sp. contamination. The result shows, of the 8 tested samples, 4 samples (sample B, C, E, and H contain little amount of borax, the sauce contains no rhodamine B. This can be seen from Rf value and color reaction test. On the testing of Salmonella sp., there are bacteria before boiling process (when cilok is still in the form of dough and there are no bacteria after boiling and steaming process, so it is safe to be consumed.

  7. Conversion of the luminescence of laser dyes in opal matrices to stimulated emission

    International Nuclear Information System (INIS)

    Alimov, O K; Basiev, T T; Orlovskii, Yu V; Osiko, V V; Samoilovich, M I

    2008-01-01

    The luminescence and laser characteristics of a synthetic opal matrix filled with organic dyes are studied upon excitation by nanosecond laser pulses. The appearance of stimulated emission in a partially ordered scattering medium is investigated. It is shown that if the luminescence spectrum of a dye (oxazine-17) is located far outside the photonic bandgap of the opal matrix, stimulated emission along a preferential direction in the (111) plane is observed when pumping exceeds a threshold even without an external optical cavity. The stimulated emission spectrum is considerably narrower than the luminescence spectrum and consists of several narrow lines located within the dye luminescence band. If the luminescence spectrum of a dye (rhodamine 6G) overlaps with the photonic bandgap of the opal matrix, a different picture is observed. The loss of radiation in the matrix leads to the red shift of the luminescence spectrum, while the stimulated emission as in the case of oxazine-17 lies is observed within the luminescence band. (active media, lasers, and amplifiers)

  8. Synthesis of an amphiphilic rhodamine derivative and characterization of its solution and thin film properties

    International Nuclear Information System (INIS)

    Aviv, Hagit; Harazi, Sivan; Schiff, Dillon; Ramon, Yoni; Tischler, Yaakov R.

    2014-01-01

    Here we present characterization of solution and thin film properties of Lissamine rhodamine B sulfonyl didodecyl amine (LRSD), an amphiphilic derivative of rhodamine. LRSD was synthesized by functionalizing Lissamine rhodamine B sulfonyl chloride (LRSC) with didodecylamine via a straightforward sulfonylation reaction. LRSD's long alkane chains make it highly soluble in chloroform, with a marked increase in brightness compared to the starting material. LRSD is shown to form well-defined robust micelles in water, without the addition of a co-surfactant and stable monolayers at the air–water interface. The greater lipophilicity of LRSD also enables doping into non-polar polymeric host matrices such as polystyrene with less aggregation and hence higher fluorescence quantum yield than LRSC or even rhodamine B. The monolayers of LRSD were prepared via Langmuir–Blodgett deposition and showed shifts in the photoluminescence peak from 575 nm to 595 nm, as the surface pressure is varied from 3 mN/m to 11 mN/m. - Highlights: • Lissamine rhodamine B sulfonyl didodecyl amine (LRSD) is soluble in chloroform. • LRSD shows robust quantum yield in solution and as a dopant in thin film. • LRSD is an amphiphilic rhodamine dye that forms compact fluorescent micelles. • LRSD forms a stable isotherm when spread at the air–water interface

  9. Electrochemical Degradation of Rhodamine B over Ti/SnO2-Sb Electrode.

    Science.gov (United States)

    Maharana, Dusmant; Niu, Junfeng; Gao, Ding; Xu, Zesheng; Shi, Jianghong

    2015-04-01

    Electrochemical degradation of rhodamine B (C28H31ClN2O3) over Ti/SnO2-Sb anode was investigated in a rectangular cell. The degradation reaction follows pseudo-first-order kinetics. The degradation efficiency of rhodamine B attained >90.0% after 20 minutes of electrolysis at initial concentrations of 5 to 200 mg/L at a constant current density of 20 mA/cm2 with a 10 mmol/L Na2SO4 supporting electrolyte solution. Rhodamine B (50 mg/L) degradation and total organic carbon (TOC) removal ratio achieved 99.9 and 86.7%, respectively, at the optimal conditions after 30 minutes of electrolysis. The results showed that the energy efficiency of rhodamine B (50 mg/L) degradation at the optimal current densities from 2 to 30 mA/cm2 were 23.2 to 84.6 Wh/L, whereas the electrolysis time for 90% degradation of rhodamine B with Ti/SnO2-Sb anode was 36.6 and 7.3 minutes, respectively. The electrochemical method can be an advisable option for the treatment of dyes such as rhodamine B in wastewater.

  10. Preparation and performance study of MgFe2O4/metal-organic framework composite for rapid removal of organic dyes from water

    Science.gov (United States)

    Tian, Huairu; Peng, Jun; Lv, Tingting; Sun, Chen; He, Hua

    2018-01-01

    In present study, a stable and magnetic metal-organic framework (MOF) material was synthesized by simple solvothermal method as adsorbent to rapid removal of two organic dyes, the Rhodamine B (RB) and Rhodamine 6G (Rh6G), in water samples. The prepared material showed great characteristics of large surface area (519.86 m2 g-1), excellent magnetic responsivity (35.00 emu g-1) and rapid removal (within 5 min). Maximum adsorption capacities of the magnetic material toward RB and Rh6G were up to 219.78 and 306.75 mg g-1, respectively. Adsorption kinetics suggested the adsorption process met the pseudo-second-order kinetic model. The prepared material could be reused at least 10 times by washing with acetonitrile solution, the relative standard deviation (RSD) of these ten cycles removal efficiency was 4.8%. In conclusion, good chemical inertness, a mechanical/water stability and super-hydrophilicity feature made this MOF a promising adsorbent for targets removal from environmental water sample.

  11. Dye laser with distributed feedback and with pumping by copper-vapor laser

    Energy Technology Data Exchange (ETDEWEB)

    Mirza, S Yu; Soldatov, A N; Sukhanov, V B

    1983-10-01

    An experimental study was made for determining the characteristics of dye lasers with distributed feedback, not requiring intricate resonator structures, and the feasibility of their pumping with radiation from a metal-vapor laser. The experiments were performed with five different dyes lasing in the yellow-red (510.6 - 578.2 nm) range of the spectrum: rhodamine 110, 6G, S and ocazine 17,1 in ethyl alcohol solution. The optical equipment included a copper-vapor pumping laser with the gas-discharge tube inside a telescopic resonator of the unstable type. Pumping pulses of 20 ns duration were generated at 510.6 and 578.2 nm wavelengths and a 4 kHz repetition rate. The pumping power was varied by means of an interference filter smoothly adjustable through rotation. The pumping laser beam was focused by a cylindrical lens on the dye cell. At optimum dye concentrations, corresponding to a maximum attainable emission power, dye concentrate was added into the circulation system for determining the dependence of the pumping threshold power on the dye concentration. Also measured were the dependence of the emission efficiency on the pumping power and the tuning range of each dye laser. The efficiency was found to remain constant over the pumping power range from threshold level to eight times higher level. The results reveal different angles of laser beam divergence in the vertical plane and in the horizontal plane, the divergence angle being four times larger in the vertical plane. The conversion efficiency increased, without significant changes in spectral characteristics, with a single annular reflector instead of two reflectors. 9 references, 4 figures, 1 table.

  12. Investigation of the lasing of dyes under copper vapor laser irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Danilova, V I; Kopylova, T N; Maier, G V; Masarnovskii, L V; Soldatov, A N; Sukhanov, V B

    1980-10-01

    The lasing characteristics of dyes pumped by copper vapor laser radiation are investigated in order to determine the optimal energetic parameters of the dye-laser system. Expressions are derived for the yields of stimulated emission from dye molecules, and it is shown that the most effective means of improving the lasing characteristics of rhodamine dye solutions is by the modification of intermolecular interactions, in part by the use of multicomponent solutions. Results are then presented of experimental measurements of the emission intensities of combinations of rhodamine dyes irradiated by the 5106-A line of a copper vapor laser. An increase in the lasing efficiency of the acceptor molecule is found for all the dye pairs investigated, with even greater emission intensities observed for multicomponent dye mixtures when the mixtures were pumped transversely. Under longitudinal pumping, improvements in lasing efficiency were obtained only for mixtures of rhodamine 6 Zh with cresil violet.

  13. Development of tunable flashlamp excited dye laser system

    International Nuclear Information System (INIS)

    Bhanthumnavin, V.; Apikitmata, S.; Kochareon, P.

    1991-01-01

    A tunable flashlamp excited dye laser (FEDL) was successfully developed for the first time in Thailand by Thai scientists at KMIT Thonburi (Bangmod). The Rhodamine 6G dissolved in ethyl alcohol was utilized as a laser medium and circulated by a pump through a laser head. The dye cuvette had an inner diameter of 4.0 mm and was 90 mm long. The cavity mirrors M 1 , and M 2 were concave mirrors with reflectivities of 100% and 73% respectively. A power supply of 0-20 kV and current of 0-50 mA charged a capacitor of 0.3 μ f at 10-15 kV which was then discharged via a spark gap through the flashlamp. The output laser wavelengths was tunable from λ = 550-640 nm. It is the first FEDL system, locally developed, which has a tunable wavelength for the laser output. The laser pulse width is about 1.0 μs with energy of 20 mJ and peak power pf 20 KW. The repetition rate of the laser is 1/15 Hz. (author). 14 refs, 7 figs

  14. Indicating pressure and environmental effects by means of the spectral shift with rhodamine B and fluorescein

    Directory of Open Access Journals (Sweden)

    R. M. Johann

    2015-07-01

    Full Text Available Fluorescence absorption and emission wavelengths can be influenced by environmental conditions, such as pressure, temperature and concentration. Here those effects are explored with an emphasis on determining the potential of rhodamine B and fluorescein as high-pressure indicators. The red shift of the emission peak maxima of rhodamine B and fluorescein are investigated in dependence of pressure up to 200 MPa using as the solvents water, ethanol and poly(dimethylsiloxane (PDMS with rhodamine B and water, polystyrene beads and melamine resin beads with fluorescein. Emission spectra recording and peak fitting is done automatically at time intervals of down to a second and with 0.3 nm wavelength resolution. The wavenumber-pressure relation for rhodamine B reveals increasing divergence from linear behavior in the sequence of the solvents water, ethanol and silicone rubber. Graphical correlation of the data diverging only slightly from linearity with a selection of polarity functions is enabled using the concept of ‘deviation from linearity (DL’ plots. Using the example of rhodamine B dissolved in PDMS elastomer it is shown that there is a temperature induced irreversible molecular reordering, when scanning between 3 and ∼50°C, and a polarity change in the proximity of the embedded dye molecule. Swelling studies are performed with PDMS containing rhodamine B, where the elastomer is first put in water, then in ethanol and again in water. There a complex solvent exchange process is revealed in the elastomer demonstrating the feasibility of fluorescence spectroscopy, when observing variations in wavelength, to indicate and enlighten molecular rearrangements and swelling dynamics in the polymer, and polarity changes and solvent exchange processes in the dye solvation shell.

  15. PENGETAHUAN DAN PERILAKU PEDAGANG CABE MERAH GILING DALAM PENGGUNAAN RHODAMINE B DI PASAR TRADISIONAL DI DKI JAKARTA

    Directory of Open Access Journals (Sweden)

    Djarismawati Djarismawati

    2012-11-01

    Full Text Available Rhodamine B, a basic dye, is prohibited to be used in foods as it is hazardous to health. Recently, it is being used to make ground red chili and oher foods more colorful and brighter. A study was carried out in DKI Jakarta to elicit information about the knowledge and practice of ground red chili vendors using Rhodamine B in three traditional markets of Jakarta. A total of 90 samples were collected from Kramat Jati, Pasar Minggu, and Tanah Abang markets. The laboratory examination result confirmed that 67% of samples contained Rhodamine B. There were 60% of respondents who knew about the health hazard of Rhodamine B and 45% of them use if to make the ground chili a brighter red. The study also showed that there was a statistical correlation between the vendors knowledge and the use of Rhodamine B. The study suggests that regular inspection and education should be conducted to improve the knowledge of ground red chili vendors, as to increase awareness of the health hazards of Rhodamine B in foods.   Keywords: knowledge, practice, red chili, Rhodamine B

  16. On the use of rhodamine WT for the characterization of stream hydrodynamics and transient storage

    Science.gov (United States)

    Runkel, Robert L.

    2015-01-01

    Recent advances in fluorometry have led to increased use of rhodamine WT as a tracer in streams and rivers. In light of this increased use, a review of the dye's behavior in freshwater systems is presented. Studies in the groundwater literature indicate that rhodamine WT is transported nonconservatively, with sorption removing substantial amounts of tracer mass. Column studies document a two-step breakthrough curve in which two structural isomers are chromatographically separated. Although the potential for nonconservative transport is acknowledged in the surface water literature, many studies assume that sorptive losses will not affect the characterization of physical transport processes. A literature review and modeling analysis indicates that this assumption is valid for quantification of physical properties that are based on the bulk of the tracer mass (traveltime), and invalid for the characterization of processes represented by the tracer tail (transient storage attributable to hyporheic exchange). Rhodamine WT should be considered nonconservative in the hyporheic zone due to nonconservative behavior demonstrated for similar conditions in groundwater. As such, rhodamine WT should not be used as a quantitative tracer in hyporheic zone investigations, including the study of long flow paths and the development of models describing hyporheic zone processes. Rhodamine WT may be used to qualitatively characterize storage in large systems, where there are few practical alternatives. Qualitative investigations should rely on early portions of the tracer profile, making use of the temporal resolution afforded by in situ fluorometry, while discarding later parts of the tracer profile that are adversely affected by sorption.

  17. Application of Taguchi L16 design method for comparative study of ability of 3A zeolite in removal of Rhodamine B and Malachite green from environmental water samples

    Science.gov (United States)

    Rahmani, Mashaallah; Kaykhaii, Massoud; Sasani, Mojtaba

    2018-01-01

    This study aimed to investigate the efficiency of 3A zeolite as a novel adsorbent for removal of Rhodamine B and Malachite green dyes from water samples. To increase the removal efficiency, effecting parameters on adsorption process were investigated and optimized by adopting Taguchi design of experiments approach. The percentage contribution of each parameter on the removal of Rhodamine B and Malachite green dyes determined using ANOVA and showed that the most effective parameters in removal of RhB and MG by 3A zeolite are initial concentration of dye and pH, respectively. Under optimized condition, the amount predicted by Taguchi design method and the value obtained experimentally, showed good closeness (more than 94.86%). Good adsorption efficiency obtained for proposed methods indicates that, the 3A zeolite is capable to remove the significant amounts of Rhodamine B and Malachite green from environmental water samples.

  18. Study of the Leacril Dyeing Process by a Cationic Dye from an Emulsion System.

    Science.gov (United States)

    Chibowski, E.; Ortega, A. Ontiveros; Espinosa-Jiménez, M.; Perea-Carpio, R.; Holysz, L.

    2001-03-15

    Adsorption studies of a cationic dye, Rhodamine B, from an emulsion phase on Leacril fabric at different temperatures were conducted. The emulsion phase consisted of n-hexadecane emulsified by isopropyl alcohol (1 M) and stabilized by tannic acid. In the alcohol solution Rhodamine B was dissolved. The kinetics of its adsorption and desorption is discussed. The changes in Leacril surface free energy components in the dyeing process were also determined. The adsorption data show that the presence of an emulsion increases the dye adsorption at room temperature (293 K) and at 313 K, while at 333 K it is smaller than that from Rhodamine solution alone. However, Rhodamine desorbs more when adsorbed from the solution. Surface free energy components differ for the Leacril samples dyed at different temperatures, and the most hydrophobic surface was obtained for the samples dyed at 333 K, where the electron-donor component is the lowest one. In general, the work of water spreading is close to zero, except for the above sample for which it is relatively highly negative. Possible mechanisms of the dye adsorption are discussed. Copyright 2001 Academic Press.

  19. Photocatalytic Degradation Property of NANO-TiO2/DIATOMITE for Rodamine B Dye Wastewater

    Science.gov (United States)

    Liu, Yue; Zheng, Shuilin; Du, Gaoxiang; Shu, Feng; Chen, Juntao

    The Nano-TiO2/Diatomite compound photocatalyst is used to degrade rhodamine B dye wastewater in photochemical reactor. The test result indicates that the rate of photodegradation of rhodamine B is influenced by reactive conditions. The best technical conditions are concentration of rhodamine B solution 10mg/L, ultraviolet light 300W, the compound photocatalyst amount used 1g/L, the pH 5.8, reaction time 20min. Under these conditions the rate of photodegradation of rhodamine B may reach as high as 97.80%. And the efficiency of photodegradation of catalyst only has a little changed in recycling.

  20. Studying reactions of interaction of tungsten (6) with rhodamine B and rhodamine 6 Zh

    International Nuclear Information System (INIS)

    Andreeva, I.Yu.; Lebedeva, L.I.; Burmistrova, N.M.

    1978-01-01

    The reaction of the interaction between tungsten (6) and rhodamine B and rhodamine 6 Zh has been investigated. The formation of two compounds in the tungsten-rhodamine system is shown. The composition of these compounds has been determined, and their stability has been estimated. The composition of compounds in the solid phase has also been determined. This reaction is used for the determination of tungsten in stells

  1. Reversible photochromic system based on rhodamine B salicylaldehyde hydrazone metal complex.

    Science.gov (United States)

    Li, Kai; Xiang, Yu; Wang, Xiaoyan; Li, Ji; Hu, Rongrong; Tong, Aijun; Tang, Ben Zhong

    2014-01-29

    Photochromic molecules are widely applied in chemistry, physics, biology, and materials science. Although a few photochromic systems have been developed before, their applications are still limited by complicated synthesis, low fatigue resistance, or incomplete light conversion. Rhodamine is a class of dyes with excellent optical properties including long-wavelength absorption, large absorption coefficient, and high photostability in its ring-open form. It is an ideal chromophore for the development of new photochromic systems. However, known photochromic rhodamine derivatives, such as amides, exhibit only millisecond lifetimes in their colored ring-open forms, making their application very limited and difficult. In this work, rhodamine B salicylaldehyde hydrazone metal complex was found to undergo intramolecular ring-open reactions upon UV irradiation, which led to a distinct color and fluorescence change both in solution and in solid matrix. The complex showed good fatigue resistance for the reversible photochromism and long lifetime for the ring-open state. Interestingly, the thermal bleaching rate was tunable by using different metal ions, temperatures, solvents, and chemical substitutions. It was proposed that UV light promoted isomerization of the rhodamine B derivative from enol-form to keto-form, which induced ring-opening of the rhodamine spirolactam in the complex to generate color. The photochromic system was successfully applied for photoprinting and UV strength measurement in the solid state. As compared to other reported photochromic molecules, the system in this study has its advantages of facile synthesis and tunable thermal bleaching rate, and also provides new insights into the development of photochromic materials based on metal complex and spirolactam-containing dyes.

  2. Characterization of Rhodamine Self-Assembled Films Using Desorption Electrospray Ionization Mass Spectrometry

    Science.gov (United States)

    Shi, Ruixia; Na, Na; Jiang, Fubin; Ouyang, Jin

    2013-06-01

    Growth process information and molecular structure identification are very important for characterization of self-assembled films. Here, we explore the possible application of desorption electrospray ionization mass spectrometry (DESI-MS) that provides the assembled information of rhodamine B (Rh B) and rhodamine 123 (Rh 123) films. With the help of lab-made DESI source, two characteristic ions [Rh B]+ and [Rh 123]+ are observed directly in the open environment. To evaluate the reliability of this technique, a comparative study of ultraviolet-visible (UV-vis) spectroscopy and our method is carried out, and the result shows good correlation. According to the signal intensity of characteristic ions, the layer-by-layer adsorption process of dyes can be monitored, and the thicknesses of multilayer films can also be comparatively determined. Combining the high sensitivity, selectivity, and speed of mass spectrometry, the selective adsorption of similar structure molecules under different pH is recognized easily from extracted ion chronograms. The variation trend of dyes signalling intensity with concentration of polyelectrolyte is studied as well, which reflects the effect of surface charge on dyes deposition. Additionally, the desorption area, surface morphology, and thicknesses of multilayer films are investigated using fluorescence microscope, scanning electron microscope (SEM), and atomic force microscopy (AFM), respectively. Because the desorption area was approximately as small as 2 mm2, the distribution situation of organic dyes in an arbitrary position could be gained rapidly, which means DESI-MS has advantages on in situ analysis.

  3. Preparation of Rhodamine B Fluorescent Poly(methacrylic acid Coated Gelatin Nanoparticles

    Directory of Open Access Journals (Sweden)

    Zhenhai Gan

    2011-01-01

    Full Text Available Poly(methacrylic acid (PMAA-coated gelatin nanoparticles encapsulated with fluorescent dye rhodamine B were prepared by the coacervation method with the aim to retard the release of rhodamine B from the gelatin matrix. With sodium sulfate as coacervation reagent for gelatin, a kind of biopolymer with excellent biocompatibility, the formed gelatin nanoparticles were cross-linked by formaldehyde followed by the polymerization of methacrylic acid coating. The fluorescent poly(methacrylic acid coated gelatin (FPMAAG nanoparticles had a uniform spherical shape and a size distribution of 60±5 nm. Infrared spectral analysis confirmed the formation of PMAA coating on the gelatin nanoparticles. Based on UV-Vis spectra, the loading efficiency of rhodamine B for the FPMAAG nanoparticles was 0.26 μg per mg nanoparticles. The encapsulated rhodamine B could sustain for two weeks. Favorable fluorescence property and fluorescence imaging of cells confirmed that the FPMAAG nanoparticles have promising biochemical, bioanalytical, and biomedical applications.

  4. Studies on the Removal of Rhodamine B and Malachite Green from Aqueous Solutions by Activated Carbon

    Directory of Open Access Journals (Sweden)

    A. Edwin Vasu

    2008-01-01

    Full Text Available Activated carbon prepared from tamarind fruit shells by direct carbonization was used for the removal of rhodamine B and malachite green dyes from aqueous solutions. Adsorption studies were performed by varying such parameters as dye concentration, pH of the dye solution, time and temperature. The equilibrium adsorption data obtained were used to calculate the Freundlich, Langmuir and Redlich-Peterson isotherm parameters. Increase in pH of the solution pH resulted in increased adsorption of both the dyes. Kinetic studies indicate that the pseudo-second order model can be used for describing the dynamics of the sorption processes. Film diffusion of the dyes was the rate determining step at low dye concentrations while diffusion of dyes through the pores the carbon particles determined the overall uptake at high concentrations. Thermodynamic parameters of the endothermic sorptions were evaluated using van’t Hoff equation. Desorption studies with acids were also performed in order to regenerate the used carbons.

  5. Characterisation of the light pulses of a cavity dumped dye laser pumped by a cw mode-locked and q-switched Nd:YAG laser

    International Nuclear Information System (INIS)

    Geist, P.; Heisel, F.; Martz, A.; Miehe, J.A.; Miller, R.J.D.

    1984-01-01

    The frequency doubled pulses (of 532 nm) obtained, with the help of a KTP crystal, from those delivered by either a continuous wave mode-locked (100 MHz) or mode-locked Q-switched (0-1 KHz) Nd: YAG laser, are analyzed by means of a streak camera, operating in synchroscan or triggered mode. In the step-by-step measurements the pulse stability, concerning form and amplitude, is shown. In addition, measurements effectuated with synchronously pumped and cavity dumped dye laser (Rhodamine 6G), controlled by a Pockels cell, allows the obtention of stable and reproducible single pulses of 30 ps duration, 10 μJ energy and 500Hz frequency [fr

  6. Nd:YAG (2 omega) pumped dye laser using self-filtering unstable resonator

    CERN Document Server

    Rahimian, K; Hariri, H

    2002-01-01

    A self-filtering unstable resonator with a magnification of M=-3 in a Nd:YAG (2 omega) dye laser has been studied. The dye solution is Rhodamine 6 G in alcohol with the concentration of 5*10 sub - 3 Mol/lit. The spatial intensity distribution of the resonator has been compared has been compared with that of a plane-parallel resonator of equal length. The output energy in both configurations are comparable (20 mu J ,and 26 mu J ,respectively). A significant difference between these two resonators is the laser beam divergence, where beam divergences of 0.77 mrad for the self-filtering unstable resonator and 1.6 mrad for the plane-parallel resonator have been measured. The brightness corresponding to these two resonators are 1.5* 10 sub 1 1 and 2.2* 10 sub 1 0W.cm sub - 2.Sr sub - 1, and the pulse widths are 7 and 17 ns, respectively. These figures show clearly that laser resonator based on the self-filtering unstable resonator design an increase the laser brightness a factor of 10, with a beam divergence of a f...

  7. Tailored Synthesis of Core-Shell Mesoporous Silica Particles—Optimization of Dye Sorption Properties

    Directory of Open Access Journals (Sweden)

    Andrzej Baliś

    2018-04-01

    Full Text Available Monodisperse spherical silica particles, with solid cores and mesoporous shells (SCMS, were synthesized at various temperatures using a one-pot method utilizing a cationic surfactant template. The temperature of the synthesis was found to significantly affect the diameters of both the cores (ca. 170–800 nm and shells (ca. 11–80 nm of the particles, which can be tailored for specific applications that require a high specific surface area of the nanocarriers (mesoporous shells and simultaneously their mechanical robustness for, e.g., facile isolation from suspensions (dense cores. The applied method enabled the formation of the relatively thick mesoporous shells at conditions below room temperature. Radially ordered pores with narrow distributions of their sizes in 3–4 nm range were found in the shells. The adsorption ability of the SCMS particles was studied using rhodamine 6G as a model dye. Decolorization of the dye solution in the presence of the SCMS particles was correlated with their structure and specific surface area and reached its maximum for the particles synthesized at 15 °C. The presented strategy may be applied for the fine-tuning of the structure of SCMS particles and the enhancement of their adsorption capabilities.

  8. Gum karaya based hydrogel nanocomposites for the effective removal of cationic dyes from aqueous solutions

    CSIR Research Space (South Africa)

    Mittala, H

    2016-02-01

    Full Text Available after nanocomposite formation with SiC NPs. The HNC was employed for the removal of cationic dyes, i.e., malachite green (MG) and rhodamine B (RhB) from the aqueous solution. The HNC was found to remove 91% (MG) and 86% (RhB) of dyes with a polymer dose...

  9. Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells

    Science.gov (United States)

    Mitronova, Gyuzel Yu.; Sidenstein, Sven C.; Klocke, Jessica L.; Kamin, Dirk; Meineke, Dirk N. H.; D'Este, Elisa; Kraemer, Philip‐Tobias; Danzl, Johann G.

    2016-01-01

    Abstract A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm. PMID:26844929

  10. Laser Dyes

    Indian Academy of Sciences (India)

    amplification or generation of coherent light waves in the UV,. VIS, and near IR region. .... ciency in most flashlamp pumped dye lasers. It is used as reference dye .... have led to superior laser dyes with increased photostabilities. For instance ...

  11. New fluorescent pH sensors based on covalently linkable PET rhodamines

    Science.gov (United States)

    Aigner, Daniel; Borisov, Sergey M.; Orriach Fernández, Francisco J.; Fernández Sánchez, Jorge F.; Saf, Robert; Klimant, Ingo

    2012-01-01

    A new class of rhodamines for the application as indicator dyes in fluorescent pH sensors is presented. Their pH-sensitivity derives from photoinduced electron transfer between non-protonated amino groups and the excited chromophore which results in effective fluorescence quenching at increasing pH. The new indicator class carries a pentafluorophenyl group at the 9-position of the xanthene core where other rhodamines bear 2-carboxyphenyl substituents instead. The pentafluorophenyl group is used for covalent coupling to sensor matrices by “click” reaction with mercapto groups. Photophysical properties are similar to “classical” rhodamines carrying 2′-carboxy groups. pH sensors have been prepared with two different matrix materials, silica gel and poly(2-hydroxyethylmethacrylate). Both sensors show high luminescence brightness (absolute fluorescence quantum yield ΦF≈0.6) and high pH-sensitivity at pH 5–7 which makes them suitable for monitoring biotechnological samples. To underline practical applicability, a dually lifetime referenced sensor containing Cr(III)-doped Al2O3 as reference material is presented. PMID:22967541

  12. A Photostable Silicon Rhodamine Platform for Optical Voltage Sensing

    Science.gov (United States)

    Huang, Yi-Lin; Walker, Alison S.; Miller, Evan W.

    2015-01-01

    This paper describes the design and synthesis of a photostable, far-red to near-infrared (NIR) platform for optical voltage sensing. We developed a new, sulfonated silicon rhodamine fluorophore and integrated it with a phenylenevinylene molecular wire to create a Berkeley Red Sensor of Transmembrane potential, or BeRST 1 (“burst”). BeRST 1 is the first member of a class of farred to NIR voltage sensitive dyes that make use of a photoinduced electron transfer (PeT) trigger for optical interrogation of membrane voltage. We show that BeRST 1 displays bright, membrane-localized fluorescence in living cells, high photostability, and excellent voltage sensitivity in neurons. Depolarization of the plasma membrane results in rapid fluorescence increases (24% ΔF/F per 100 mV). BeRST 1 can be used in conjunction with fluorescent stains for organelles, Ca2+ indicators, and voltage-sensitive fluorescent proteins. In addition, the red-shifted spectral profile of BeRST 1, relative to commonly employed optogenetic actuators like ChannelRhodopsin2 (ChR2), which require blue light, enables optical electrophysiology in neurons. The high speed, sensitivity, photostability and long-wavelength fluorescence profiles of BeRST 1 make it a useful platform for the non-invasive, optical dissection of neuronal activity. PMID:26237573

  13. Peculiarities of the statistics of spectrally selected fluorescence radiation in laser-pumped dye-doped random media

    Science.gov (United States)

    Yuvchenko, S. A.; Ushakova, E. V.; Pavlova, M. V.; Alonova, M. V.; Zimnyakov, D. A.

    2018-04-01

    We consider the practical realization of a new optical probe method of the random media which is defined as the reference-free path length interferometry with the intensity moments analysis. A peculiarity in the statistics of the spectrally selected fluorescence radiation in laser-pumped dye-doped random medium is discussed. Previously established correlations between the second- and the third-order moments of the intensity fluctuations in the random interference patterns, the coherence function of the probe radiation, and the path difference probability density for the interfering partial waves in the medium are confirmed. The correlations were verified using the statistical analysis of the spectrally selected fluorescence radiation emitted by a laser-pumped dye-doped random medium. Water solution of Rhodamine 6G was applied as the doping fluorescent agent for the ensembles of the densely packed silica grains, which were pumped by the 532 nm radiation of a solid state laser. The spectrum of the mean path length for a random medium was reconstructed.

  14. Sensitive chemiluminescence immunoassay for staphylococcal enterotoxin C1 based on the use of dye-encapsulated mesoporous silica nanoparticles

    International Nuclear Information System (INIS)

    Tao, Liang; Zhang, Chunmei; Sun, Yuanjie; Jin, Boquan; Yang, Kun; Li, Xiaohua; Zhang, Zhujun; Zhang, Jinpeng; Yan, Kuocheng

    2016-01-01

    A chemiluminescent immunoassay for the staphylococcal enterotoxin C1 (SEC1) based on the use of dye-encapsulated mesoporous silica nanoparticles (m-SiNPs) as a label is described. The dyes are retained in the m-SiNPs via strong hydrophobic interactions. The assay comprises the following steps: (a) Microplates coated with antibody against SEC1 are filled with sample upon which the SEC antigen will be bound to the surface; (b) following a washing step, secondary antibody linked to m-SiNPs (that were covalently labeled with rhodamine 6G and fluorescein) were added to form the sandwich complex; (c) after another washing step, bis(2,4,6-trichlorophenyl) oxalate, H_2O_2 and imidazole are added to generate chemiluminescence whose intensity is proportional to the number of m-SiNPs and thus to the number of antigen (SEC) molecules. It is found that the use of functionalized m-SiNPs strongly amplifies the signal. Enterotoxin SEC1 can be detected by this method in the 0.025 to 2 ng⋅mL"-"1 concentration range, the detection limit is 19 pg⋅mL"-"1 (at 3σ), and the relative standard deviation (for 11 parallel measurements at a 1 ng⋅mL"-"1 level) is 4.6 %. The use of an automated chemiluminescence analyzer further improves detection. (author)

  15. Synthesis of Tb_4O_7 complexed with reduced graphene oxide for Rhodamine-B absorption

    International Nuclear Information System (INIS)

    Gao, Hui; Zhou, Yang; Chen, Keqin; Li, Xiaolong

    2016-01-01

    Highlights: • Tb–rGO composite was fabricated via a facile thermally reduction process. • The green and blue emissions were both observed in the composite. • The composite exhibited efficient absorption capability for Rhodamine-B. - Abstract: Tb_4O_7 complexed with reduced graphene oxide composite (Tb–rGO) had been designed and fabricated by a facile thermal reduction method. The formation of Tb_4O_7 particles and reduction of graphene oxide (GO) occurred simultaneously, and partial terbium ions would be complexed with rGO via oxygen-containing function groups on rGO sheets. Introducing of terbium ions could effectively tune the photoluminescence properties of rGO, and the composite exhibited the typical green emission of terbium ions as well as the blue self-luminescence of graphene entered at 440 nm. Moreover, Tb–rGO had demonstrated its high capability as an organic dye (Rhodamine-B) scavenger with high speed and efficiency. The findings showed the promising applications for large-scale removal of organic dye contaminants, especially in the field of waste water treatment.

  16. A study on linear and non-linear optical constants of Rhodamine B thin film deposited on FTO glass

    Science.gov (United States)

    Yahia, I. S.; Jilani, Asim; Abutalib, M. M.; AlFaify, S.; Shkir, M.; Abdel-wahab, M. Sh.; Al-Ghamdi, Attieh A.; El-Naggar, A. M.

    2016-06-01

    The aim of this research was to fabricate/deposit the good quality thin film of Rhodamine B dye on fluorine doped tin oxide glass substrate by the low cost spin coating technique and study their linear and nonlinear optical parameters. The thickness of the thin film was measured about 300 nm with alpha step system. The transmittance of the fabricated thin film was found to be above 75% corresponding to the fluorine doped tin oxide layer. The structural analysis was performed with X-rays diffraction spectroscopy. Atomic force microscope showed the topographic image of deposited thin film. Linear optical constant like absorption coefficient, band gap, and extinction index was calculated. The dielectric constant was calculated to know the optical response of Rhodamine B dye over fluorine doped tin oxide substrate. The nonlinear optical constant like linear optical susceptibility χ(1), nonlinear optical susceptibility χ(3), nonlinear refractive index (n2) were calculated by spectroscopic method. This method has advantage over the experimental method like Z-Scan for organic dye base semiconductors for future advance optoelectronics applications like dye synthesis solar cell.

  17. A study on linear and non-linear optical constants of Rhodamine B thin film deposited on FTO glass

    Energy Technology Data Exchange (ETDEWEB)

    Yahia, I.S. [Nano-Science & Semiconductor Labs, Physics Department, Faculty of Education, Ain Shams University, Roxy, Cairo (Egypt); Department of Physics, Faculty of Science, King Khalid University, P.O. Box 9004, Abha (Saudi Arabia); Jilani, Asim, E-mail: asim.jilane@gmail.com [Centre of Nanotechnology, Physics Department-Faculty of Science-AL Faisaliah Campus, King Abdulaziz University, P.O. Box 80200, Jeddah 21589 (Saudi Arabia); Abutalib, M.M. [Centre of Nanotechnology, Physics Department-Faculty of Science-AL Faisaliah Campus, King Abdulaziz University, P.O. Box 80200, Jeddah 21589 (Saudi Arabia); AlFaify, S. [Nano-Science & Semiconductor Labs, Physics Department, Faculty of Education, Ain Shams University, Roxy, Cairo (Egypt); Shkir, M. [Department of Physics, Faculty of Science, King Khalid University, P.O. Box 9004, Abha (Saudi Arabia); Abdel-wahab, M.Sh.; Al-Ghamdi, Attieh A. [Centre of Nanotechnology, Physics Department-Faculty of Science-AL Faisaliah Campus, King Abdulaziz University, P.O. Box 80200, Jeddah 21589 (Saudi Arabia); El-Naggar, A.M. [Exploitation of Renewable Energy Applications in Saudi Arabia, Physics & Astronomy Department, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451 (Saudi Arabia)

    2016-06-01

    The aim of this research was to fabricate/deposit the good quality thin film of Rhodamine B dye on fluorine doped tin oxide glass substrate by the low cost spin coating technique and study their linear and nonlinear optical parameters. The thickness of the thin film was measured about 300 nm with alpha step system. The transmittance of the fabricated thin film was found to be above 75% corresponding to the fluorine doped tin oxide layer. The structural analysis was performed with X-rays diffraction spectroscopy. Atomic force microscope showed the topographic image of deposited thin film. Linear optical constant like absorption coefficient, band gap, and extinction index was calculated. The dielectric constant was calculated to know the optical response of Rhodamine B dye over fluorine doped tin oxide substrate. The nonlinear optical constant like linear optical susceptibility χ{sup (1)}, nonlinear optical susceptibility χ{sup (3)}, nonlinear refractive index (n{sub 2}) were calculated by spectroscopic method. This method has advantage over the experimental method like Z-Scan for organic dye base semiconductors for future advance optoelectronics applications like dye synthesis solar cell.

  18. A Study on the Interaction of Rhodamine B with Methylthioadenosine Phosphorylase Protein Sourced from an Antarctic Soil Metagenomic Library

    Science.gov (United States)

    Bujacz, Anna; Wierzbicka-Woś, Anna; Kur, Józef

    2013-01-01

    The presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified and its UV-VIS absorbance spectrum and emission spectrum were assayed. Simultaneously, the same spectroscopic studies were carried out for rhodamine B in the absence or presence of RSFP protein or native E. coli proteins, respectively. The results of the spectroscopic studies suggested that the fluorescence of E. coli cells expressing rsfp gene under UV illumination is due to the interaction of rhodamine B molecules with the RSFP protein. Finally, this interaction was proved by a crystallographic study and then by site-directed mutagenesis of rsfp gene sequence. The crystal structures of RSFP apo form (1.98 Å) and complex RSFP/RB (1.90 Å) show a trimer of RSFP molecules located on the crystallographic six fold screw axis. The RSFP complex with rhodamine B revealed the binding site for RB, in the pocket located on the interface between symmetry related monomers. PMID:23383268

  19. Development of a rapid, simple and sensitive HPLC-FLD method for determination of rhodamine B in chili-containing products.

    Science.gov (United States)

    Qi, Ping; Lin, Zhihao; Li, Jiaxu; Wang, ChengLong; Meng, WeiWei; Hong, Hong; Zhang, Xuewu

    2014-12-01

    In this work, a simple, rapid and sensitive analytical method for the determination of rhodamine B in chili-containing foodstuffs is described. The dye is extracted from samples with methanol and analysed without further cleanup procedure by high-performance liquid chromatography (HPLC) coupled to fluorescence detection (FLD). The influence of matrix fluorescent compounds (capsaicin and dihydrocapsaicin) on the analysis was overcome by the optimisation of mobile-phase composition. The limit of determination (LOD) and limit of quantification (LOQ) were 3.7 and 10 μg/kg, respectively. Validation data show a good repeatability and within-lab reproducibility with relative standard deviations rhodamine B in foodstuffs. This method is suitable for the routine analysis of rhodamine B due to its sensitivity, simplicity, reasonable time and cost. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Effect of Tannic Acid on the zeta Potential, Sorption, and Surface Free Energy in the Process of Dyeing of Leacril with a Cationic Dye.

    Science.gov (United States)

    Espinosa-Jiménez; Giménez-Martín; Ontiveros-Ortega

    1998-11-01

    The behavior of the surface free energy in the process of dyeing Leacril pretreated with tannic acid and subsequently dyeing with the cationic dye Rhodamine B has been studied. Also the electrokinetic behavior of these systems has been analyzed by studying the zeta potential, which has been obtained by means of the streaming potential technique. Values more significative of the zeta potential of these systems have been obtained using the three models of capillaries existing in the literature. The qualitative behavior of the zeta potential is the same for the three models of capillaries tested in this paper. These models are those of Goring and Mason, Biefer and Mason, and Chang and Robertson. The zeta potential of the systems analyzed is negative in the range of concentration of the dye in the liquid phase from 10(-6) to ca. 10(-4) M of dye. In the range of low concentrations (from 10(-6) to ca. 10(-5) M of dye) the zeta potential of the system untreated Leacril/Rhodamine B increases in absolute value due to increasing hydrophobic attractions between both the hydrophobic chains of the dye and the Leacril fibers in aqueous media. In the system Leacril treated with tannic acid/Rhodamine B, this increase is also due to the presence of hydrogen bonding between the phenolic hydroxyl groups of the tannic acid and the sulfonate and sulfate end groups of Leacril fibers. For concentrations of dye between 10(-5) and 10(-4) M of dye in solution, the zeta potential decreases in absolute value due to the electrostatic attractions between the groups negatively charged in the fiber and the cation of the dye. The zeta potential changes its sign at the highest concentrations of dye used in this work. The adsorption of Rhodamine B onto both untreated Leacril and Leacril treated with tannic acid is favored by the increasing temperature of adsorption. The behavior of the components of the surface free energy obtained by the thin-layer wicking technique led us to consider that the

  1. Ly6G-mediated depletion of neutrophils is dependent on macrophages.

    Science.gov (United States)

    Bruhn, Kevin W; Dekitani, Ken; Nielsen, Travis B; Pantapalangkoor, Paul; Spellberg, Brad

    2016-01-01

    Antibody-mediated depletion of neutrophils is commonly used to study neutropenia. However, the mechanisms by which antibodies deplete neutrophils have not been well defined. We noticed that mice deficient in complement and macrophages had blunted neutrophil depletion in response to anti-Ly6G monoclonal antibody (MAb) treatment. In vitro, exposure of murine neutrophils to anti-Ly6G MAb in the presence of plasma did not result in significant depletion of cells, either in the presence or absence of complement. In vivo, anti-Ly6G-mediated neutrophil depletion was abrogated following macrophage depletion, but not complement depletion, indicating a requirement for macrophages to induce neutropenia by this method. These results inform the use and limitations of anti-Ly6G antibody as an experimental tool for depleting neutrophils in various immunological settings.

  2. Blocking of Single α-Hemolysin Pore by Rhodamine Derivatives.

    Science.gov (United States)

    Rokitskaya, Tatyana I; Nazarov, Pavel A; Golovin, Andrey V; Antonenko, Yuri N

    2017-06-06

    Measurements of ion conductance through α-hemolysin pore in a bilayer lipid membrane revealed blocking of the ion channel by a series of rhodamine 19 and rhodamine B esters. The longest dwell closed time of the blocking was observed with rhodamine 19 butyl ester (C4R1), whereas the octyl ester (C8R1) was of poor effect. Voltage asymmetry in the binding kinetics indicated that rhodamine derivatives bound to the stem part of the aqueous pore lumen. The binding frequency was proportional to a quadratic function of rhodamine concentrations, thereby showing that the dominant binding species were rhodamine dimers. Two levels of the pore conductance and two dwell closed times of the pore were found. The dwell closed times lengthened as the voltage increased, suggesting impermeability of the channel for the ligands. Molecular docking analysis revealed two distinct binding sites within the lumen of the stem of the α-hemolysin pore for the C4R1 dimer, but only one binding site for the C8R1 dimer. The blocking of the α-hemolysin nanopore by rhodamines could be utilized in DNA sequencing as additional optical sensing owing to bright fluorescence of rhodamines if used for DNA labeling. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  3. BiVO4 -TiO2 Composite Photocatalysts for Dye Degradation Formed Using the SILAR Method.

    Science.gov (United States)

    Odling, Gylen; Robertson, Neil

    2016-09-19

    Composite photocatalyst films have been fabricated by depositing BiVO4 upon TiO2 via a sequential ionic layer adsorption reaction (SILAR) method. The photocatalytic materials were investigated by XRD, TEM, UV/Vis diffuse reflectance, inductively coupled plasma optical emission spectrometry (ICP-OES), XPS, photoluminescence and Mott-Schottky analyses. SILAR processing was found to deposit monoclinic-scheelite BiVO4 nanoparticles onto the surface, giving successive improvements in the films' visible light harvesting. Electrochemical and valence band XPS studies revealed that the prepared heterojunctions have a type II band structure, with the BiVO4 conduction band and valence band lying cathodically shifted from those of TiO2 . The photocatalytic activity of the films was measured by the decolourisation of the dye rhodamine 6G using λ>400 nm visible light. It was found that five SILAR cycles was optimal, with a pseudo-first-order rate constant of 0.004 min(-1) . As a reference material, the same SILAR modification has been made to an inactive wide-band-gap ZrO2 film, where the mismatch of conduction and valence band energies disallows charge separation. The photocatalytic activity of the BiVO4 -ZrO2 system was found to be significantly reduced, highlighting the importance of charge separation across the interface. The mechanism of action of the photocatalysts has also been investigated, in particular the effect of self-sensitisation by the model organic dye and the ability of the dye to inject electrons into the photocatalyst's conduction band. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Bimanes and Related Heterocycles as Laser Dyes

    Science.gov (United States)

    1991-01-10

    mixture that contained no more than a trace ig-Bridged syn-Bimanes amount of a solid thought to be a mononitro deriv- ative on the basis of M+ 209 in...disappeared and the solution became yellow. ether, ethanol, ethyl acetate, ethyl benzylacetoace- Traces of chlorine were removed by purging with a tate...methyl phosphite . Coumarins 6, 30, 120, and 314, and neutral alumina (2:3 by wt) was added to the rhodamine 6G, sulforhodamine B and thin layer

  5. Single longitudinal mode operation of a solid-state dye laser oscillator

    CERN Document Server

    Lim, G; Kim, H S; Cha, B H; Lee, J M

    2000-01-01

    We have operated a single longitudinal mode of a solid-state dye laser oscillator in a Littman configuration. The host material of the solid-state gain medium was rhodamine dye-doped poly (methyl methacrylate). The pumping source was the second harmonic of a Nd:YAG laser with a repetition rate of 10 Hz. The measured linewidth of the laser output was about 1.5 GHz.

  6. Single bead near-infrared random laser based on silica-gel infiltrated with Rhodamine 640

    Science.gov (United States)

    Moura, André L.; Barbosa-Silva, Renato; Dominguez, Christian T.; Pecoraro, Édison; Gomes, Anderson S. L.; de Araújo, Cid B.

    2018-04-01

    Photoluminescence properties of single bead silica-gel (SG) embedded with a laser-dye were studied aiming at the operation of near-infrared (NIR) Random Lasers (RLs). The operation of RLs in the NIR spectral region is especially important for biological applications since the optical radiation has deep tissue penetration with negligible damage. Since laser-dyes operating in the NIR have poor stability and are poor emitters, ethanol solutions of Rhodamine 640 (Rh640) infiltrated in SG beads were used. The Rh640 concentrations in ethanol varied from 10-5 to 10-2 M and the excitation at 532 nm was made by using a 7 ns pulsed laser. The proof-of-principle RL scheme herein presented was adopted in order to protect the dye-molecules from the environment and to favor formation of aggregates. The RL emission from ≈650 nm to 720 nm, beyond the typical Rh640 monomer and dimer wavelengths emissions range, was attributed to the trade-off between reabsorption and reemission processes along the light pathways inside the SG bead and the contribution of Rh640 aggregates.

  7. Investigation of adsorption of Rhodamine B onto a natural adsorbent Argemone mexicana.

    Science.gov (United States)

    Khamparia, Shraddha; Jaspal, Dipika

    2016-12-01

    The present study aims at exploring the potential of the seeds of a tropical weed, Argemone mexicana (AM), for the removal of a toxic xanthene textile dye, Rhodamine B (RHB), from waste water. Impact of pH, adsorbent dosage, particle size, contact time and dye concentration have been assessed during adsorption. The weed has been well characterized by several latest techniques thereby providing an indepth information of the mechanism during adsorption. About 80% removal has been attained with 0.06 g of adsorbent over the studied system. Thermodynamic and kinetic studies, followed by second order kinetic model, directed towards the endothermic nature of adsorption. The results obtained from batch experiments were modelled using Langmuir and Freundlich isotherm and were analysed on the basis of R 2 and six error functions for selection of appropriate model. Langmuir isotherm was found to be best fitted to the experimental data with high values of R 2 and lower values of error functions. Adsorption study revealed the affinity of AM seeds for the dye ions present in waste water, introducing a novel adsorbent in field of waste water treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. [11 C]Rhodamine-123: Synthesis and biodistribution in rodents

    International Nuclear Information System (INIS)

    Bao Xiaofeng; Lu Shuiyu; Liow, Jeih-San; Morse, Cheryl L.; Anderson, Kacey B.; Zoghbi, Sami S.; Innis, Robert B.; Pike, Victor W.

    2012-01-01

    Introduction: Rhodamine-123 is a known substrate for the efflux transporter, P-glycoprotein (P-gp). We wished to assess whether rhodamine-123 might serve as a useful substrate for developing probes for imaging efflux transporters in vivo with positron emission tomography (PET). For this purpose, we aimed to label rhodamine-123 with carbon-11 (t 1/2 = 20.4 min) and to study its biodistribution in rodents. Methods: [ 11 C]Rhodamine-123 was prepared by treating rhodamine-110 (desmethyl-rhodamine-123) with [ 11 C]methyl iodide. The biodistribution of this radiotracer was studied with PET in wild-type mice and rats, in efflux transporter knockout mice, in wild-type rats pretreated with DCPQ (an inhibitor of P-gp) or with cimetidine (an inhibitor of organic cation transporters; OCT), and in P-gp knockout mice pretreated with cimetidine. Unchanged radiotracer in forebrain, plasma and peripheral tissues was also measured ex vivo at 30 min after radiotracer administration to wild-type and efflux transporter knockout rodents. Results: [ 11 C]Rhodamine-123 was obtained in 4.4% decay-corrected radiochemical yield from cyclotron-produced [ 11 C]carbon dioxide. After intravenous administration of [ 11 C]rhodamine-123 to wild-type rodents, PET and ex vivo measurements showed radioactivity uptake was very low in brain, but relatively high in some other organs such as heart, and especially liver and kidney. Inhibition of P-gp increased uptake in brain, heart, kidney and liver, but only by up to twofold. Secretion of radioactivity from kidney was markedly reduced by OCT knockout or pretreatment with cimetidine. Conclusions: [ 11 C]Rhodamine-123 was unpromising as a PET probe for P-gp function and appears to be a strong substrate of OCT in kidney. Cimetidine appears effective for blocking OCT in kidney in vivo.

  9. Application of activated carbon derived from scrap tires for adsorption of Rhodamine B.

    Science.gov (United States)

    Li, Li; Liu, Shuangxi; Zhu, Tan

    2010-01-01

    Activated carbon derived from solid hazardous waste scrap tires was evaluated as a potential adsorbent for cationic dye removal. The adsorption process with respect to operating parameters was investigated to evaluate the adsorption characteristics of the activated pyrolytic tire char (APTC) for Rhodamine B (RhB). Systematic research including equilibrium, kinetics and thermodynamic studies was performed. The results showed that APTC was a potential adsorbent for RhB with a higher adsorption capacity than most adsorbents. Solution pH and temperature exert significant influence while ionic strength showed little effect on the adsorption process. The adsorption equilibrium data obey Langmuir isotherm and the kinetic data were well described by the pseudo second-order kinetic model. The adsorption process followed intra-particle diffusion model with more than one process affecting the adsorption process. Thermodynamic study confirmed that the adsorption was a physisorption process with spontaneous, endothermic and random characteristics.

  10. Studies of rhodamine-123: effect on rat prostate cancer and human prostate cancer cells in vitro.

    Science.gov (United States)

    Arcadi, J A; Narayan, K S; Techy, G; Ng, C P; Saroufeem, R M; Jones, L W

    1995-06-01

    The effect of the lipophilic, cationic dye, Rhodamine-123 (Rh-123), on prostate cancer in rats, and on three tumor cell lines in vitro is reported here. The general toxicity of Rh-123 in mice has been found to be minimal. Lobund-Wistar (L-W) rats with the autochthonous prostate cancer of Pollard were treated for six doses with Rh-123 at a dose of 15 mg/kg subcutaneously every other day. Microscopic examination of the tumors revealed cellular and acinar destruction. The effectiveness of Rh-123 as a cytotoxic agent was tested by clonogenic and viability assays in vitro with three human prostate cancer cell lines. Severe (60-95%) growth inhibition was observed following Rh-123 exposure for 2-5 days at doses as low as 1.6 micrograms/ml in all three prostate cancer cell lines.

  11. Identifikasi Zat Warna Rhodamine B Pada Lipstik Berwarna Merah

    OpenAIRE

    A.H., Mukaromah; E.T., Maharani

    2008-01-01

    Telah dilakukan penelitian tentang identifikasi zat warna Rhodamine B pada lipsttk. Populasi Penelitian adalah sediaan lipstik yang berwarna merah dengan harga kurang atau sama dengan Rp 5000,00 (12 merk) dan sediaan lipstik yang berwarna merah dengan harga lebih Rp 5000,00 (13 merk) yang dijual di Pasar Johar Semarang. Masing-masing sediaan lipstik diambil 40 % dari total populasi secara acak. Metode yang digunakan untuk mangidentifikasi zat warna Rhodamine B adalah metode kromatograf...

  12. Development of the plastic solid-dye cell for tunable solid-state dye lasers and study on its optical properties

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Do Kyeong; Lee, Jong Min; Cha, Byung Heon; Yi, Jong Hoon; Lee, Kang Soo; Kim, Sung Ho; Lim, Gwon

    2000-01-01

    We have fabricated solid-state dyes with Copolex NK-55, which is the base element of plastic lens, and PMMA. We have measured the longevity of solid-state dyes doped in both polymers and found that PMMA has better properties than Coploex NK-55. We have realized the tuning range of 560-620 nm by doping rhodamine 6Gand rhodamin B in the manufactured solid-state dye laser oscillators. In the standing-wave cavity we achieved the slop efficiency of 10.8 percent and in the grazing incidence cavity, 1.2 percent. We have constructed a very compact grazing- incidence cavity which is only 6 cm long and the linewidth of the laser was less than 1.5 GHz with 3-ns pulse duration. And we have fabricated disk-type solid-state dye cell and installed it in the cavity in which the dye cell can be translated and rotated with the help of the two steeping motors. By this we could constantly changed the illuminated area of the dye cell and , therefore, were able to achieve long time operation and to use almost the entire region of the solid-state dye cell. (author)

  13. Synthesis of Tb{sub 4}O{sub 7} complexed with reduced graphene oxide for Rhodamine-B absorption

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Hui, E-mail: hope@lzu.edu.cn [School of Physical Science and Technology, Key Laboratory for Magnetism and Magnetic Materials of Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Zhou, Yang; Chen, Keqin [School of Physical Science and Technology, Key Laboratory for Magnetism and Magnetic Materials of Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Li, Xiaolong, E-mail: lixiaolong@sinap.ac.cn [Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204 (China)

    2016-05-15

    Highlights: • Tb–rGO composite was fabricated via a facile thermally reduction process. • The green and blue emissions were both observed in the composite. • The composite exhibited efficient absorption capability for Rhodamine-B. - Abstract: Tb{sub 4}O{sub 7} complexed with reduced graphene oxide composite (Tb–rGO) had been designed and fabricated by a facile thermal reduction method. The formation of Tb{sub 4}O{sub 7} particles and reduction of graphene oxide (GO) occurred simultaneously, and partial terbium ions would be complexed with rGO via oxygen-containing function groups on rGO sheets. Introducing of terbium ions could effectively tune the photoluminescence properties of rGO, and the composite exhibited the typical green emission of terbium ions as well as the blue self-luminescence of graphene entered at 440 nm. Moreover, Tb–rGO had demonstrated its high capability as an organic dye (Rhodamine-B) scavenger with high speed and efficiency. The findings showed the promising applications for large-scale removal of organic dye contaminants, especially in the field of waste water treatment.

  14. Dynamic adsorption of mixtures of Rhodamine B, Pb (II), Cu (II) and Zn(II) ions on composites chitosan-silica-polyethylene glycol membrane

    Science.gov (United States)

    Mahatmanti, F. W.; Rengga, W. D. P.; Kusumastuti, E.; Nuryono

    2018-04-01

    The adsorption of a solution mixture of Rhodamine B, Pb (II), Cu (II) and Zn(II) was studied using dynamic methods employing chitosan-silica-polyethylene glycol (Ch/Si/P) composite membrane as an adsorptive membrane. The composite Ch/Si/P membrane was prepared by mixing a chitosan-based membrane with silica isolated from rice husk ash (ASP) and polyethylene glycol (PEG) as a plasticizer. The resultant composite membrane was a stronger and more flexible membrane than the original chitosan-based membrane as indicated by the maximum percentage of elongation (20.5 %) and minimum Young’s Modulus (80.5 MPa). The composite membrane also showed increased mechanical and hydrophilic properties compared to the chitosan membranes. The membrane was used as adsorption membrane for Pb (II), Cu (II), Cd (II) ions and Rhodamine B dyes in a dynamic system where the permeation and selectivity were determined. The permeation of the components was observed to be in the following order: Rhodamine B > Cd (II) > Pb (II) > Cu (II) whereas the selectivity was shown to decrease the order of Cu (II) > Pb (II) > Cd (II) > Rhodamine B.

  15. Polymer optical fiber with Rhodamine doped cladding for fiber light systems

    Energy Technology Data Exchange (ETDEWEB)

    Narro-García, R., E-mail: roberto.narro@gmail.com [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Quintero-Torres, R. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Domínguez-Juárez, J.L. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Cátedras CONACyT, Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico); Ocampo, M.A. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230 (Mexico)

    2016-01-15

    Both preform and polymer optical fiber with a Poly(methyl methacrylate) core and THV–Rhodamine 6G cladding were characterized. UV–vis absorbance, photoluminescence spectra and lifetime of the preform were measured. Axial and lateral photoluminescence spectra of the polymer optical fiber were studied under 404 nm excitation in order to study the illumination performance of the fiber. It was observed that the peak wavelength from the fiber photoluminescence spectra is higher than the peak wavelength from the fiber preform and that the peak wavelength from the fiber photoluminescence spectra is red shifted with the fiber length in the case of axial emission. The obtained results suggest the influence of self-absorption on the photoluminescence shape. Strong lateral emission along the fiber was observed with the naked eyes in all the cases. The lateral photoluminescence spectra show that the lateral emission is a combination between the pump laser and the Rh6G molecule photoluminescence. The results suggest that this polymer optical fiber could be a potential candidate for the development of fiber lighting systems. - Highlights: • Axial and lateral emission along the fiber was studied. • Self-absorption effect was confirmed in the case of axial photoluminescence. • The lateral emission is a combination between the laser and the RhG6 emission. • This fiber could be a potential candidate for the development of lighting systems.

  16. Adsorption of rhodamine B by acid activated carbon-Kinetic, thermodynamic and equilibrium studies

    Directory of Open Access Journals (Sweden)

    Shanmugam Arivoli

    2009-08-01

    Full Text Available A carbonaceous adsorbent prepared from an indigenous waste by acid treatment was tested for its efficiency in removing Rhodamine B (RDB. The parameters studied include agitation time, initial dye concentration, carbon dose, pH and temperature. The adsorption followed first order kinetics and the rate is mainly controlled by intra-particle diffusion. Freundlich and Langmuir isotherm models were applied to the equilibrium data. The adsorption capacity (Qm obtained from the Langmuir isotherm plots were 40.161, 35.700, 38.462 and 37.979 mg/g respectively at an initial pH of 7.0 at 30, 40, 50 and 60 0C. The temperature variation study showed that the RDB adsorption is endothermic and spontaneous with increased randomness at the solid solution interface. Significant effect on adsorption was observed on varying the pH of the RDB solutions. Almost 85% removal of RDB was observed at 60 0C. The Langmuir and Freundlich isotherms obtained, positive ?H0 value, pH dependent results and desorption of dye in mineral acid suggest that the adsorption of RDB by Banana bark carbon involves physisorption mechanism.

  17. Endogenous morphine-6-glucuronide (M6G) is present in the plasma of patients: validation of a specific anti-M6G antibody for clinical and basic research.

    Science.gov (United States)

    Laux-Biehlmann, Alexis; Chung, Hélène; Mouheiche, Jinane; Vérièpe, Julie; Delalande, François; Lamshöft, Marc; Welters, Ingeborg D; Soldevila, Stéphanie; Bazin, Hervé; Lamarque, Laurent; Van Dorsselaer, Alain; Poisbeau, Pierrick; Schneider, Francis; Goumon, Yannick; Garnero, Patrick

    2014-01-01

    Endogenous morphine and its derivatives (morphine-6-glucuronide [M6G]; morphine-3-glucuronide [M3G]) are formed by mammalian cells from dopamine. Changes in the concentrations of endogenous morphine have been demonstrated in several pathologies (sepsis, Parkinson's disease, etc.), and they might be relevant as pathological markers. While endogenous morphine levels are detectable using enzyme-linked immunosorbant assay (ELISA), mass spectrometry (MS) analysis was, so far, the only approach to detect and quantify M6G. This study describes the preparation of a specific anti-M6G rabbit polyclonal antibody and its validation. The specificity of this antibody was assessed against 30 morphine-related compounds. Then, a M6G-specific ELISA-assay was tested to quantify M6G in the plasma of healthy donors, morphine-treated, and critically ill patients. The antibody raised against M6G displays a strong affinity for M6G, codeine-6-glucuronide, and morphine-3-6-glucuronide, whereas only weak cross-reactivities were observed for the other compounds. Both M6G-ELISA and LC-MS/MS approaches revealed the absence of M6G in the plasma of healthy donors (controls, n = 8). In all positive donors treated with morphine-patch (n = 5), M6G was detected using both M6G-ELISA and LC-MS/MS analysis. Finally, in a study on critically ill patients with circulating endogenous morphine (n = 26), LC-MS/MS analysis revealed that 73% of the positive-patients (19 of 26), corresponding to high M6G-levels in M6G-ELISA, contained M6G. In conclusion, we show that endogenous M6G can be found at higher levels than morphine in the blood of morphine-naive patients. With respect to the interest of measuring endogenous M6G in pathologies, we provide evidences that our ELISA procedure represents a powerful tool as it can easily and specifically detect endogenous M6G levels. © 2013 International Union of Biochemistry and Molecular Biology.

  18. Nano-dyeing

    Directory of Open Access Journals (Sweden)

    Ning Cui-Juan

    2016-01-01

    Full Text Available Dyeing nanofibers is a frontier of both modern textile engineering and nanotechnology. This paper suggest a feasible method for dyeing nanofibers with a natural red (Roselle Calyx by bubble electrospinning. Reactive dye (Red S3B and acid dye (Red 2B were also used in the experiment for comparison. The dyeing process was finished during the spinning process.

  19. Anomalous dependence of the lasing parameters of dye solutions on the spectrum of microsecond pump laser pulses

    International Nuclear Information System (INIS)

    Tarkovsky, V V; Kurstak, V Yu; Anufrik, S S

    2003-01-01

    The anomalous dependence of the lasing parameters of ethanol solutions of coumarin, rhodamine, oxazine, and laser dyes of other classes on the spectrum of microsecond pump laser pulses is found. The dependence is determined by the shape of the induced singlet - singlet absorption spectra and absorption spectra of short-lived photoproducts. The elucidation of the influence of these factors makes it possible to choose optimal pump spectra and to enhance the efficiency and stability of microsecond dye lasers. (active media)

  20. Random laser emission at dual wavelengths in a donor-acceptor dye mixture solution

    Directory of Open Access Journals (Sweden)

    Sunita Kedia

    Full Text Available The work was aimed to generate random laser emissions simultaneously at two wavelengths in a weakly scattering system containing mixture of binary dyes, rhodamine-B (Rh-B and oxazine-170 (O-170 dispersed with ZnO nano-particles serving as scattering centres. Random lasing performances for individual Rh-B dye were extensively studied for varying small signal gain/scatterer density and we found lasing threshold to significantly depend upon number density of dispersed nano-particles. In spite of inefficient pumping, we demonstrated possibility of random lasing in O-170 dye solution on account of resonance energy transfer from Rh-B dye which served as donor. At optimum concentrations of fluorophores and scatterer in dye mixture solution, incoherent random lasing was effectively attained simultaneously at two wavelengths centered 90 nm apart. Dual-emission intensities, lasing thresholds and rate of amplifications could be controlled and made equivalent for both donor and acceptor in dye mixture solution by appropriate choice of concentrations of dyes and scatterers. Keywords: Random lasing, Energy transfer, Rhodamine-B, Oxazine-170, Zinc oxide

  1. Noninvasive control of rhodamine-loaded capsules distribution in vivo

    Science.gov (United States)

    Stelmashchuk, O.; Tarakanchikova, Y.; Seryogina, E.; Piavchenko, G.; Zherebtsov, E.; Dunaev, A.; Popov, A.; Meglinski, I.

    2018-04-01

    Using fluorescence spectroscopy system with fibre-optical probe, we investigated the dynamics of propagation and circulation in the microcirculatory system of experimental nanocapsules fluorescent-labelled (rhodamine TRITC) nanocapsules. The studies were carried out in clinically healthy Wistar rats. The model animals were divided into control group and group received injections of the nanocapsules. The fluorescent measurements conducted transcutaneously on the thigh surface. The administration of the preparation with the rhodamine concentration of 5 mg/kg of animal weight resulted in twofold increase of fluorescence intensity by reference to the baseline level. As a result of the study, it was concluded that fluorescence spectroscopy can be used for transdermal measurements of the rhodamine-loaded capsules in vivo.

  2. Hair dye poisoning

    Science.gov (United States)

    Hair tint poisoning ... Different types of hair dye contain different harmful ingredients. The harmful ingredients in permanent dyes are: Naphthylamine Other aromatic amino compounds Phenylenediamines Toluene ...

  3. Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.

    Science.gov (United States)

    Johnson, Brian J; Mitchell, Sara N; Paton, Christopher J; Stevenson, Jessica; Staunton, Kyran M; Snoad, Nigel; Beebe, Nigel; White, Bradley J; Ritchie, Scott A

    2017-09-01

    Recent interest in male-based sterile insect technique (SIT) and incompatible insect technique (IIT) to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success) during field releases. Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v) in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males. These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis) and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males.

  4. Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.

    Directory of Open Access Journals (Sweden)

    Brian J Johnson

    2017-09-01

    Full Text Available Recent interest in male-based sterile insect technique (SIT and incompatible insect technique (IIT to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success during field releases.Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males.These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males.

  5. Selective extraction-photometric determination of cadmium by basic dyes

    Energy Technology Data Exchange (ETDEWEB)

    Kish, P P; Balog, J S [Uzhgorodskij Gosudarstvennyj Univ. (Ukrainian SSR)

    1979-12-01

    Two variants of selective extraction-photometric determination of cadmium with basic dyes have been developed. In the first one, cadmium is extracted as the iodide by a tributyl phosphate solution in benzene from aqueous solutions containing 0.1 M KI (pH 6-10). Then the cadmium is transformed into a coloured ion associate by treatment of the extracts with Malachite Green in the presence of iodide ions. In the second case, the extract is equilibrated with an equeous solutions of Rhodamine B in the presence of KBr. In this variant, the cadmium is transformed into an anionic iodide-bromide complex which reacts with Rhodamine B cations to form an ion associate. Procedures have been developed of selective extraction-photometric determination of cadmium in sulphur, indium-gallium and zinc concentrates, Zn-As-Cd-Se and Zn-As-Cd-Te films, Cd-S-In and Ga-Sb-Cd-Te alloys.

  6. Spiroguanidine rhodamines as fluorogenic probes for lysophosphatidic acid

    Science.gov (United States)

    Wang, Lei; Sibrian-Vazquez, Martha; Escobedo, Jorge O.; Wang, Jialu; Moore, Richard G.

    2015-01-01

    Direct determination of total lysophosphatidic acid (LPA) was accomplished using newly developed spiroguanidines derived from rhodamine B as universal fluorogenic probes. Optimum conditions for the quantitative analysis of total LPA were investigated. The linear range for the determination of total LPA is up to 5 μM with a limit of detection of 0.512 μM. PMID:25516957

  7. Solvent effects on lasing characteristics for Rh B laser dye

    Energy Technology Data Exchange (ETDEWEB)

    Peter, Jaison, E-mail: jaison.peter@gmail.com [International School of Photonics, Cochin University of Science and Technology, Cochin 682022 (India); Kumar, Mahesh [Department of Applied Chemistry, Cochin University of Science and Technology, Cochin 682022 (India); Ananad, V.R.; Saleem, Rasool; Sebastian, Ananthu; Radhakrishnan, P.; Nampoori, V.P.N.; Vallabhan, C.P.G. [International School of Photonics, Cochin University of Science and Technology, Cochin 682022 (India); Prabhu, Radhakrishna [School of Engineering, Robert Gordon University, Aberdeen AB10 1FR, Scotland (United Kingdom); Kailasnath, M. [International School of Photonics, Cochin University of Science and Technology, Cochin 682022 (India)

    2016-01-15

    We demonstrate pulsed, photopumped multimode laser emission in the visible spectral range from rhodamine B dye dissolved in various solvents. The laser emission is characterized by a well-defined, low threshold pump power at which the emission spectral intensity dramatically increases and collapsed into several dominant laser modes with reduced mode spacing and spectral width. The modes were found to originate from the subcavities formed by the plane-parallel walls of the cuvette containing the gain medium. The cavity lasing spectral structure and the numbers of longitudinal modes were easily controlled by changing the solvents. A shift in the emission spectra has been also observed by changing the solvents will allow a limited range of tuning of laser emission wavelength. We also determined the gain coefficient and stimulated emission cross-section for the Rh B dye dissolved liquid laser system. A detailed discussion of the solvent effect in the lasing characteristics of Rh B in different solution is explained along with the computational data. - Highlights: • Report multimode laser emission from rhodamine B dye dissolved in various solvents. • Modes are originated from the plane-parallel walls of the cuvette. • Spectral range and the number of modes can be controlled by changing the solvents. • Changing solvents also allows a limited range of tuning of laser emission.

  8. Preparation and Utilization of Kapok Hull Carbon for the Removal of Rhodamine-B from Aqueous Solution

    Directory of Open Access Journals (Sweden)

    P. S. Syed Shabudeen

    2006-01-01

    Full Text Available A carbonaceous sorbent prepared from the indegeneous agricultural waste (which is facing solid waste disposal problem Kapok Hull, by acid treatment was tested for its efficiency in removing basic dyes. Batch kinetic and isotherm experiments were conducted to determine the sorption and desorption of the Rhodamine-B from aqueous solution with activated carbon. The factors affecting the rate processes involved in the removal of dye for initial dye concentration, agitation time, and carbon dose and particle size have been studied at ambient temperature. The adsorption process followed first order rate kinetics. The first-order rate equation by Lagergren was tested on the kinetic data, and isotherm data was analyzed for possible agreement with the Langmuir and Freundlich adsorption isotherm equations. The intraparticle diffusion rate equation from which adsorption rate constants, diffusion rate constants and diffusion coefficients were determined. Intraparticle diffusion was found to be the rate-limiting step. The structural and morphological of activated carbon were characterized by XRD and SEM studies respectively.

  9. A novel fluorescent probe based on rhodamine hydrazone derivatives bearing a thiophene group for Al³⁺.

    Science.gov (United States)

    Li, Meng-xiao; Zhang, Xia; Fan, Yu-hua; Bi, Cai-feng

    2016-05-01

    In the present work, a novel 5-methyl-thiophene-carbaldehyde-functionalized rhodamine 6G Schiff base (RA) was designed and easily prepared as an Al(3+) fluorescent and colorimetric probe, which could selectively and sensitively detect Al(3+) by showing enhanced fluorescence emission. Meanwhile distinct color variation from colorless to pink also provided 'naked eye' detection of Al(3+), due to the ring spirolactam opening of the rhodamine derivative. Other metal ions (including K(+), Mg(2+), Na(+), Ba(2+), Mn(2+), Cd(2+), Fe(2+), Ni(2+), Pb(2+), Zn(2+), Hg(2+), Co(2+), Li(+), Sr(2+) and Cu(2+)) could only induce limited interference. The detection limit of the fluorescent probe was estimated to be 4.17 × 10(-6) M, the binding constant of the RA-Al(3+) complex was 1.4 × 10(6)  M(-1). Moreover, this fluorescent probe RA possessed high reversibility. As aluminum is a ubiquitous metal in nature and plays vital roles in many biological processes, this chemosensor could be explored for biological study applications. Copyright © 2015 John Wiley & Sons, Ltd.

  10. Anaerobic azo dye reduction

    OpenAIRE

    Zee, van der, F.P.

    2002-01-01

    Azo dyes, aromatic moieties linked together by azo (-N=N-) chromophores, represent the largest class of dyes used in textile-processing and other industries. The release of these compounds into the environment is undesirable, not only because of their colour, but also because many azo dyes and their breakdown products are toxic and/or mutagenic to life. To remove azo dyes from wastewater, a biological treatment strategy based on anaerobic reduction of the azo dye...

  11. Pharmacokinetics and Biodistribution of the Illegal Food Colorant Rhodamine B in Rats.

    Science.gov (United States)

    Cheng, Yung-Yi; Tsai, Tung-Hu

    2017-02-08

    The International Agency for Research on Cancer (IARC) demonstrated rhodamine B as a potential carcinogen in 1978. Nevertheless, rhodamine B has been illegally used as a colorant in food in many countries. Few pharmacokinetic and toxicological investigations have been performed since the first pharmacokinetic study on rhodamine B in 1961. The aims of this study were to develop a simple and sensitive high-performance liquid chromatography method with fluorescence detection for the quantitative detection of rhodamine B in the plasma and organs of rats and to estimate its pharmacokinetics and biodistribution. The results demonstrated that the oral bioavailabilities of rhodamine B were 28.3 and 9.8% for the low-dose and high-dose exposures, respectively. Furthermore, rhodamine B was highly accumulated in the liver and, to a lesser extent, the kidney, but was undetectable in the brain. These results provide useful information for improving the pharmacokinetics and biodistribution of rhodamine B, supporting additional food safety evaluations.

  12. Kinetic, Thermodynamic and Equilibrium Studies on Uptake of Rhodamine B onto ZnCl2 Activated Low Cost Carbon

    Directory of Open Access Journals (Sweden)

    N. Bhadusha

    2012-01-01

    Full Text Available A carbonaceous adsorbent prepared from biomass waste like wood apple outer shell (Limonia acidissima by ZnCl2 treatment was investigated for its efficiency in removing Rhodamine B (RDB. Influence of agitation time, adsorbent dose, dye concentration, pH and temperature were explored. Two theoretical adsorption isotherms namely Langmuir and Freundlich were used to describe the experimental results. The Langmuir adsorption capacity (Qo was found to be 46.7 mg/g and the equilibrium parameter (RL values indicate favourable adsorption. The experimental data were well fitted with Langmuir isotherm model and pseudo second order kinetic model. Desorption studies showed that ion exchange mechanism might be involved in the adsorption process.

  13. Fabrication and characterization of microcavity lasers in rhodamine B doped SU8 using high energy proton beam

    Science.gov (United States)

    Venugopal Rao, S.; Bettiol, A. A.; Vishnubhatla, K. C.; Bhaktha, S. N. B.; Narayana Rao, D.; Watt, F.

    2007-03-01

    The authors present their results on the characterization of individual dye-doped microcavity polymer lasers fabricated using a high energy proton beam. The lasers were fabricated in rhodamine B doped SU8 resist with a single exposure step followed by chemical processing. The resulting trapezoidal shaped cavities had dimensions of ˜250×250μm2. Physical characterization of these structures was performed using a scanning electron microscope while the optical characterization was carried out by recording the emission subsequent to pumping the lasers with 532nm, 6 nanosecond pulses. The authors observed intense, narrow emission near 624nm with the best emission linewidth full width at half maximum of ˜9nm and a threshold ˜150μJ/mm2.

  14. Central Shops Burning/Rubble Pit 631-6G Additional Sampling and Monitor Well Installation Report

    International Nuclear Information System (INIS)

    Palmer, E.

    1995-02-01

    The Central Shops Burning/Rubble Pit 631-6G was constructed in 1951 as an unlined earthen pit in surficial sediments for disposal and incineration of potentially hazardous substances, such as metals and organic solvents

  15. Decolourization of Rhodamine B: A swirling jet-induced cavitation combined with NaOCl.

    Science.gov (United States)

    Mancuso, Giuseppe; Langone, Michela; Laezza, Marco; Andreottola, Gianni

    2016-09-01

    A hydrodynamic cavitation reactor (Ecowirl) based on swirling jet-induced cavitation has been used in order to allow the degradation of a waste dye aqueous solution (Rhodamine B, RhB). Cavitation generated by Ecowirl reactor was directly compared with cavitation generated by using multiple hole orifice plates. The effects of operating conditions and parameters such as pressure, pH of dye solution, initial concentration of RhB and geometry of the cavitating devices on the degradation rate of RhB were discussed. In similar operative conditions, higher extents of degradation (ED) were obtained using Ecowirl reactor rather than orifice plate. An increase in the ED from 8.6% to 14.7% was observed moving from hole orifice plates to Ecowirl reactor. Intensification in ED of RhB by using hydrodynamic cavitation in presence of NaOCl as additive has been studied. It was found that the decolourization was most efficient for the combination of hydrodynamic cavitation and chemical oxidation as compared to chemical oxidation and hydrodynamic cavitation alone. The value of ED of 83.4% was reached in 37min using Ecowirl combined with NaOCl (4.0mgL(-1)) as compared to the 100min needed by only mixing NaOCl at the same concentration. At last, the energetic consumptions of the cavitation devices have been evaluated. Increasing the ED and reducing the treatment time, Ecowirl reactor resulted to be more energy efficient as compared to hole orifice plates, Venturi and other swirling jet-induced cavitation devices, as reported in literature. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Photocatalytic decolorization of basic dye by TiO2 nanoparticle in photoreactor

    Directory of Open Access Journals (Sweden)

    Jutaporn Chanathaworn1

    2012-04-01

    Full Text Available Photocatalytic decolorization of rhodamine B (RB and malachite green (MG basic dyes in aqueous solution wasevaluated using TiO2 powder as a semiconductor photocatalyst under UV black light irradiation. A 0.5 L batch photoreactorcontaining dyeing solution was installed in a stainless steel chamber with air cooling under irradiation. The TiO2 powder wascharacterized by XRD observation and it was shown that the nanoparticles could be identified as 73 nm anatase crystals. Theeffects of operational parameters such as light intensity (0-114 W/m2, initial dye concentration (10-30 mg/L, and TiO2 powderloading (0.5-1.5 g/L on the decolorization of dye samples were examined. The photocatalytic decolorization rate depended onthe pollutant’s structure, such that the MG dye could be removed faster than the RB dye. Decolorization efficiency (% of thephotocatalytic system increased with increasing TiO2 loading and light intensity; however, it decreased with increasing initialdye concentration. A loading of 1.5 g TiO2/L, initial dye concentration of 20 mg/L, and light intensity of 114 W/m2 were foundto yield the highest removal efficiency of dye solution based on time requirement. The kinetics are of first order and dependon the TiO2 powder loading and dye structure. The research had a perfect application foreground.

  17. Removal of methylene blue and rhodamine B from water by zirconium oxide/graphene

    Directory of Open Access Journals (Sweden)

    Sumita Rani

    2016-04-01

    Full Text Available Methylene blue (MB and rhodamine B dyes (RB were degraded from water using zirconium oxide (ZrO2 and zirconium oxide/graphene composites (ZrO2/GR as photocatalyst. The photocatalytic efficiency was calculated from absorption spectra obtained using UV–visible spectroscopy. It has been observed that photodegradation time as well as photocatalytic efficiency increase with the concentration of catalyst up to a certain limit after which effect was reversed. The degradation was studied as a function of pH also. It was found that photocatalytic efficiency was more in alkaline medium than acidic medium. Degradation of RB takes place at higher value of pH as compared to MB. The degradation time for MB was 1 h using ZrO2 which get reduced to 32 min using ZrO2/GR composite and for RB it reduced to 40 min (using ZrO2/GR from 80 min (ZrO2.

  18. Interaction of Lysozyme with Rhodamine B: A combined analysis of spectroscopic & molecular docking.

    Science.gov (United States)

    Millan, Sabera; Satish, Lakkoji; Kesh, Sandeep; Chaudhary, Yatendra S; Sahoo, Harekrushna

    2016-09-01

    The interaction of Rhodamine B (RB) with Lysozyme (Lys) was investigated by different optical spectroscopic techniques such as absorption, fluorescence, and circular-dichroism (CD), along with molecular docking studies. The fluorescence results (including steady-state and time-resolved mode) revealed that the addition of RB effectively causes strong quenching of intrinsic fluorescence in Lysozyme and mostly, by the static quenching mechanism. Different binding and thermodynamic parameters were calculated at different temperatures and the binding constant value was found to be 2963.54Lmol(-1) at 25°C. The average distance (r0) was found to be 3.31nm according to Förster's theory of non-radiative energy transfer between Lysozyme and RB. The conformational change in Lysozyme during interaction with RB was confirmed from absorbance, synchronous fluorescence, and circular dichroism measurements. Finally, molecular docking studies were done to confirm that the dye binds with Lysozyme. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Tunable Microfluidic Dye Laser

    DEFF Research Database (Denmark)

    Olsen, Brian Bilenberg; Helbo, Bjarne; Kutter, Jörg Peter

    2003-01-01

    We present a tunable microfluidic dye laser fabricated in SU-8. The tunability is enabled by integrating a microfluidic diffusion mixer with an existing microfluidic dye laser design by Helbo et al. By controlling the relative flows in the mixer between a dye solution and a solvent......, the concentration of dye in the laser cavity can be adjusted, allowing the wavelength to be tuned. Wavelength tuning controlled by the dye concentration was demonstrated with macroscopic dye lasers already in 1971, but this principle only becomes practically applicable by the use of microfluidic mixing...

  20. Energy transfer in porous anodic alumina/rhodamine 110 nanocomposites

    Energy Technology Data Exchange (ETDEWEB)

    Elhouichet, H., E-mail: habib.elhouichet@fst.rnu.tn [Laboratoire de Physico-Chimie des Materiaux Mineraux et leurs Applications, Centre National de Recherches en Sciences des Materiaux, B.P. 95, Hammam-Lif 2050 (Tunisia); Departement de Physique, Faculte des Sciences de Tunis, University of Tunis Elmanar 2092 Tunis (Tunisia); Harima, N.; Koyama, H. [Hyogo University of Teacher Education, Kato, Hyogo 673-1494 (Japan); Gaponenko, N.V. [Belarusian State University of Informatics and Radioelectronics, P. Browki St. 6, 220013 Minsk (Belarus)

    2012-09-15

    We have used porous anodic alumina (PAA) films as templates for embedding rhodamine 110 (Rh110) molecules and examined their photoluminescence (PL) properties in detail. The analysis of the polarization memory (PM) of PL strongly suggests that there is a significant energy transfer from PAA to Rh110 molecules. The effect of annealing the PAA layer on the PL properties of the nanocomposite has been studied. The results show that the energy transfer becomes more efficient in annealed PAA. - Highlights: Black-Right-Pointing-Pointer Porous anodic alumina-rhodamine 110 nanocomposites are elaborated. Black-Right-Pointing-Pointer Efficient energy transfer from the host to Rh110 molecules is evidenced from measurements of photoluminescence and degree of polarization memory spectra. Black-Right-Pointing-Pointer Thermal annealing of porous anodic alumina can improve the process of excitation transfer.

  1. Extended rhodamine photosensitizers for photodynamic therapy of cancer cells.

    Science.gov (United States)

    Davies, Kellie S; Linder, Michelle K; Kryman, Mark W; Detty, Michael R

    2016-09-01

    Extended thio- and selenorhodamines with a linear or angular fused benzo group were prepared. The absorption maxima for these compounds fell between 640 and 700nm. The extended rhodamines were evaluated for their potential as photosensitizers for photodynamic therapy in Colo-26 cells. These compounds were examined for their photophysical properties (absorption, fluorescence, and ability to generate singlet oxygen), for their dark and phototoxicity toward Colo-26 cells, and for their co-localization with mitochondrial-specific agents in Colo-26 and HUT-78 cells. The angular extended rhodamines were effective photosensitizers toward Colo-26 cells with 1.0Jcm(-2) laser light delivered at λmax±2nm with values of EC50 of (2.8±0.4)×10(-7)M for sulfur-containing analogue 6-S and (6.4±0.4)×10(-8)M for selenium-containing analogue 6-Se. The linear extended rhodamines were effective photosensitizers toward Colo-26 cells with 5 and 10Jcm(-2) of broad-band light (EC50's⩽2.4×10(-7)M). Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Fast adsorption kinetics of highly dispersed ultrafine nickel/carbon nanoparticles for organic dye removal

    Science.gov (United States)

    Kim, Taek-Seung; Song, Hee Jo; Dar, Mushtaq Ahmad; Lee, Hack-Jun; Kim, Dong-Wan

    2018-05-01

    Magnetic metal/carbon nano-materials are attractive for pollutant adsorption and removal. In this study, ultrafine nickel/carbon nanoparticles are successfully prepared via electrical wire explosion processing in ethanol media for the elimination of pollutant organic dyes such as Rhodamine B and methylene blue in aqueous solutions. High specific surface areas originating from both the nano-sized particles and the existence of carbon on the surface of Ni nanoparticles enhance dye adsorption capacity. In addition to this, the excellent dispersity of Ni/C nanoparticles in aqueous dye solutions leads to superior adsorption rates. The adsorption kinetics for the removal of organic dyes by Ni/C nanoparticles agree with a pseudo-second-order model and follow Freundlich adsorption isotherm behavior.

  3. Chemistry of Natural Dyes

    Indian Academy of Sciences (India)

    scientific principles, and the interaction between the dye and the dyed material is ... Dyes are classified based on their structure, source, method of application .... the right source that gives not only beautiful tones, but colourfast shades as well.

  4. A validated LC-MS/MS determination method for the illegal food additive rhodamine B: Applications of a pharmacokinetic study in rats.

    Science.gov (United States)

    Cheng, Yung-Yi; Tsai, Tung-Hu

    2016-06-05

    Rhodamine B is an illegal and potentially carcinogenic food dye. The aim of this study was to develop a convenient, rapid, and sensitive UHPLC-MS/MS method for pharmacokinetic studies in rats. Rat plasma samples were deproteinized with acetonitrile and separated by UHPLC on a reverse-phase C18e column (100mm×2.1mm, 2μm) using a mobile phase consisting of methanol-5mM ammonium acetate (90:10, v/v). Detection was performed using a triple quadrupole tandem mass spectrometer in the selected reaction monitoring mode at [M](+) ion m/z 443.39→399.28 for rhodamine B and [M+H](+) ion m/z 253.17→238.02 for 5-methoxyflavone as the internal standard. This method was specific and produced linear results over a concentration range of 0.5-100ng/mL, with a lower limit of quantitation of 0.5ng/mL. All validation parameters, including the inter-day, intra-day, matrix effect, recovery, and stability in rat plasma, were acceptable according to the biological method validation guidelines developed by the FDA (2001). This method was successfully applied to a pharmacokinetic study in rats; oral administration of 1mg/kg of rhodamine B yielded a time to maximum concentration (Tmax) of 1.3±0.4h and an elimination half-life of 8.8±1.4h, with a clearance of 229.7±19.4mL/h/kg. These pharmacokinetic results provide a constructive contribution to our understanding of the absorption mechanism of rhodamine B and support additional food safety evaluations. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. uv dye lasers

    International Nuclear Information System (INIS)

    Abakumov, G.A.; Fadeev, V.V.; Khokhlov, R.V.; Simonov, A.P.

    1975-01-01

    The most important property of visible dye lasers, that is, continuous wavelength tuning, stimulated the search for dyes capable to lase in uv. They were found in 1968. Now the need for tunable uv lasers for applications in spectroscopy, photochemistry, isotope separation, remote air and sea probing, etc. is clearly seen. A review of some recent advances in uv dye lasers is reviewed

  6. Effect of porphyrin on photocatalytic activity of TiO2 nanoparticles toward Rhodamine B photodegradation.

    Science.gov (United States)

    Ahmed, M A; Abou-Gamra, Z M; Medien, H A A; Hamza, M A

    2017-11-01

    As known, porphyrins have central role in photosynthesis, biological oxidation and reduction and oxygen transport beside to their intensive color which qualify them to be good photosensitizers. Herein, tetra (4-carboxyphenyl) porphyrin (TCPP) was prepared by a simple one-pot synthesis to use as a visible antenna for TiO 2 nanoparticles that were prepared via a simple template-free sol-gel method. Various loading percentages of TCPP (0.05-1%) were incorporated on the surface of TiO 2 as photosensitizer for photocatalytic degradation of Rhodamine B (Rh B) dye as a primary cationic pollutant model. Among them, 0.1% TCPP-TiO 2 was the most reactive sample. It was found that the photoactivity of 0.1% TCPP-TiO 2 sample (0.5g/L) was approximately 1.5 times greater than that of pure TiO 2 (0.5g/L) toward the degradation of Rh B (1×10 -5 M) under UV-A irradiation. Transient fluorescence decay measurements showed that the life time of TiO 2 excited state has doubled after anchoring TCPP, thus the probability of electron-hole recombination has decreased. The samples were characterized by XRD, HR-TEM, DRS and N 2 adsorption-desorption isotherms. The XRD patterns confirmed the successful preparation of TiO 2 nanoparticles with average crystalline size of 25.7nm. Also, XRD patterns suggested the presence of mixed phase TiO 2 nanoparticles of 77% anatase and 23% rutile. DRS showed that the characteristic peaks of TCPP covered the whole visible range 400-700nm. HR-TEM images showed the spheroids shape of TiO 2 nanoparticles and confirmed the presence of anatase and rutile phases as suggested from XRD data. The different parameters affecting the photodegradation of Rh B dye such as catalyst dose, dye concentration and pH were studied to obtain the optimum conditions. Almost complete degradation of Rh B was obtained which confirmed by HPLC and TOC measurements. The effect of scavengers was studied to indicate the most active species. TCPP-TiO 2 gave a good response toward the

  7. Daylight-driven photocatalytic degradation of ionic dyes with negatively surface-charged In{sub 2}S{sub 3} nanoflowers: dye charge-dependent roles of reactive species

    Energy Technology Data Exchange (ETDEWEB)

    Ge, Suxiang [Xuchang University, Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, and School of Chemistry and Chemical Engineering (China); Cai, Lejuan, E-mail: 494169965@qq.com [Central China Normal University, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry (China); Li, Dapeng, E-mail: lidapengabc@126.com; Fa, Wenjun; Zhang, Yange; Zheng, Zhi [Xuchang University, Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, and School of Chemistry and Chemical Engineering (China)

    2015-12-15

    Even though dye degradation is a successful application of semiconductor photocatalysis, the roles of reactive species in dye degradation have not received adequate attention. In this study, we systematically investigated the degradation of two cationic dyes (rhodamine B and methylene blue) and two anionic dyes (methyl orange and orange G) over negatively surface-charged In{sub 2}S{sub 3} nanoflowers synthesized at 80 °C under indoor daylight lamp irradiation. It is notable to find In{sub 2}S{sub 3} nanoflowers were more stable in anionic dyes degradation compared to that in cationic dyes removal. The active species trapping experiments indicated photogenerated electrons were mainly responsible for cationic dyes degradation, but holes were more important in anionic dyes degradation. A surface-charge-dependent role of reactive species in ionic dye degradation was proposed for revealing such interesting phenomenon. This study would provide a new insight for preparing highly efficient daylight-driven photocatalyst for ionic dyes degradation.

  8. Hair dye contact allergy

    DEFF Research Database (Denmark)

    Søsted, Heidi; Rastogi, Suresh Chandra; Andersen, Klaus Ejner

    2004-01-01

    Colouring of hair can cause severe allergic contact dermatitis. The most frequently reported hair dye allergens are p-phenylenediamine (PPD) and toluene-2,5-diamine, which are included in, respectively, the patch test standard series and the hairdressers series. The aim of the present study...... was to identify dye precursors and couplers in hair dyeing products causing clinical hair dye dermatitis and to compare the data with the contents of these compounds in a randomly selected set of similar products. The patient material comprised 9 cases of characteristic clinical allergic hair dye reaction, where...... exposure history and patch testing had identified a specific hair dye product as the cause of the reaction. The 9 products used by the patients were subjected to chemical analysis. 8 hair dye products contained toluene-2,5-diamine (0.18 to 0.98%). PPD (0.27%) was found in 1 product, and m-aminophenol (0...

  9. Construction of a photovoltaic cell based on the photoelectrochemistry of organic dyes at transparent semi-conducting electrodes. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Nasielski, J; Kirsch-De Mesmaeker, A

    1982-01-01

    It is shown that the study of the photoelectrochemical mechanisms of different dye-reductant systems at transparent semi-conducting bubbling gas electrodes, coupled with a detailed analysis of the photovoltammetric curves, allows: a deeper insight into the operation principles of the corresponding dye photogalvanic cells; prediction of the parameters important for optimizing the cell operation; and working out of strategies for improving the cell output. In order to compare the behaviors of an adsorbed type dye (rhodamine-hydroquinone) with a solution type dye in a photogalvanic cell, the thionine-Fe/sup 2 +/ system was examined, including a detailed kinetic analysis of the photocurrents at the bubbling gas electrode as a function of several parameters. Photoelectrochemical mechanisms related to the sensitization and supersensitization in this system have thus been determined. Photoelectrochemical methods were applied to analyze other dye-reductant systems. The photoelectrochemisty of three triphenyl-methanes, methyl violet, crystal violet, and malachite green were examined at the bubbling gas electrode. These dyes produce photocurrents originating from the adsorbed molecules and behave very much like rhodamine. The photoelectrochemical behavior of the erythrosine-hydroquinone couple is also examined. For constructing photogalvanic cells, the CdS-on-SnO/sub 2/-cysteine system is found promising. (LEW)

  10. Regenerable, innovative porous silicon-based polymer-derived ceramics for removal of methylene blue and rhodamine B from textile and environmental waters.

    Science.gov (United States)

    Bruzzoniti, Maria Concetta; Appendini, Marta; Onida, Barbara; Castiglioni, Michele; Del Bubba, Massimo; Vanzetti, Lia; Jana, Prasanta; Sorarù, Gian Domenico; Rivoira, Luca

    2018-04-01

    The presence of residual color in treated textile wastewater above the regulation limits is still a critical issue in many textile districts. Innovative, polymer-derived ceramics of the Si-C-O system were here synthesized in order to obtain porous nanocomposite materials where a free carbon phase is dispersed into a silicon carbide/silicon oxycarbide network. The sorbents were comprehensively characterized for the removal of two model water-soluble dyes (i.e., the cation methylene blue and the zwitterion rhodamine B). Adsorption is very rapid and controlled by intra-particle and/or film diffusion, depending on dye concentration. Among the nanocomposites studied, the SiOC aerogel (total capacity about 45 mg/g, is easily regenerated under mild treatment (250 °C, 2 h). Adsorption of dyes is not affected by the matrix composition: removals of 150 mg/L methylene blue from river water and simulated textile wastewater with high content of metal ions (2-50 mg/L) and chemical oxygen demand (800 mg/L) were higher than 92% and quantitative for a dye concentration of 1 mg/L.

  11. Fluorescence quenching of Rhodamine B base by two amines

    Science.gov (United States)

    Bakkialakshmi, S.; Selvarani, P.; Chenthamarai, S.

    2013-03-01

    Fluorescence quenching of Rhodamine B base (RhB) in DMF solution has been studied at different concentrations of the amine Triethyl amine (TEA) and n-butyl amine (NBA) at room temperature. It has been observed that the fluorescence intensity of RhB decrease with increase in the concentration of the TEA and NBA. It has been observed that the quenching due to amines proceeds via dynamic quenching process. The rate constants for the quenching process have been calculated using Stern-Volmer equation. Time resolved fluorescence study and 1H NMR spectral study have also been carried out and discussed.

  12. A new method for dosing rhodamine B in natural water

    International Nuclear Information System (INIS)

    Marichal, M.; Benoit, R.

    1961-01-01

    A simple and sensitive method well adapted to hydrological research. The dye is first extracted from the water sample by isoamyl alcohol and then the fluorescence of the alcoholic solution, after excitation by ultraviolet radiation, is measured spectrophotometrically. The sensitivity of the method is about 10 -12 , that is, a millionth of a milligram of dye per litre. Reprint of a paper published in 'Chimie Analytique', N. 2, Feb 1962, p. 70-72 [fr

  13. Rhodamine B in dissolved and nano-bound forms: Indicators for light-based advanced oxidation processes.

    Science.gov (United States)

    Shabat-Hadas, Efrat; Mamane, Hadas; Gitis, Vitaly

    2017-10-01

    Rhodamine B (RhB) is a water-soluble fluorescent dye that is often used to determine flux and flow direction in biotechnological and environmental applications. In the current research, RhB in soluble (termed free) and virus-bound (termed nano-bound) forms was used as an efficiency indicator for three environmental processes. The degradation of free and nano-bound RhB by (i) direct UV photolysis and (ii) UV/H 2 O 2 advanced oxidation process (AOP) was studied in a collimated beam apparatus equipped with medium-pressure mercury vapor lamp. The degradation by (iii) solar light-induced photocatalysis was studied in a solar simulator with titanium dioxide and bismuth photocatalysts. Results showed negligible RhB degradation by direct UV and solar light, and its nearly linear degradation by UV/H 2 O 2 and photocatalysis/photosensitization in the presence of a solid catalyst. Considerable adsorption of free RhB on bismuth-based catalyst vs. no adsorption of nano-bound RhB on this catalyst or of any form of the dye on titanium dioxide produced two important conclusions. First, the better degradation of free RhB by the bismuth catalyst suggests that close proximity of a catalyst hole and the decomposing molecule significantly influences degradation. Second, the soluble form of the dye might not be the best option for its use as an indicator. Nano-bound RhB showed high potential as an AOP indicator, featuring possible separation from water after the analysis. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Plasmonic effects of gold colloids on the fluorescence behavior of dye-doped SiO{sub 2} nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Tarpani, Luigi, E-mail: luigi.tarpani@unipg.it; Latterini, Loredana

    2017-05-15

    The interactions of dye molecules with gold nanoparticles are of great interest owing to the potential applications in the areas of bioimaging, sensing and photodynamic therapy applications. In many cases the distances between fluorophores and the metal particles can change during the experiment and the spectral features of the units are not taken into account. In this work, the fluorescence behaviour of two dyes with different spectral properties (Rhodamine B and 9-aminoacridine) are investigated in the presence of gold nanoparticles having diameters of 2 or 26 nm and hence different plasmonic properties. In order to fix the distance between the dye and the gold nanoparticles, the dyes are entrapped in 20 nm silica nanoparticles, and the metal colloids are adsorbed on the silica surface. The distance between the fluorescent units and the metal particles is tuned by growing additional silica layers on the pristine nanoparticles. Steady-state and time-resolved fluorescence measurements show that in the presence of gold nanoparticles, having 2 nm diameter, a drastic quenching of the dye emission is observed, for all the prepared samples, despite the average dye-metal distances. When gold nanoparticles with 26 nm diameters are used, their interactions with the dyes are strongly dependent on the averaged distances between the metal colloids and the dyes and on the overlap of their spectral properties. Indeed, an enhanced emission is observed for 9-aminoacridine while the fluorescence of longer wavelength emitting Rhodamine B is quenched. The steady state and time-resolved data are analysed to evaluate the plasmonic impact of the radiative and non-radiative rate constants of the dyes.

  15. On different photodecomposition behaviors of rhodamine B on laponite and montmorillonite clay under visible light irradiation

    KAUST Repository

    Wang, Peng; Cheng, Mingming; Zhang, Zhonghai

    2013-01-01

    In this study, laponite and montmorillonite clays were found to be able to decompose rhodamine B upon visible light irradiation (λ>420nm). Very interestingly, it was found that rhodamine B on laponite underwent a stepwise N-deethylation and its

  16. Study of decolorisation of binary dye mixture by response surface methodology.

    Science.gov (United States)

    Khamparia, Shraddha; Jaspal, Dipika

    2017-10-01

    Decolorisation of a complex mixture of two different classes of textile dyes Direct Red 81 (DR81) and Rhodamine B (RHB), simulating one of the most important condition in real textile effluent was investigated onto deoiled Argemone Mexicana seeds (A. Mexicana). The adsorption behaviour of DR81 and RHB dyes was simultaneously analyzed in the mixture using derivative spectrophotometric method. Central composite design (CCD) was employed for designing the experiments for this complex binary mixture where significance of important parameters and possible interactions were analyzed by response surface methodology (RSM). Maximum adsorption of DR81 and RHB by A. Mexicana was obtained at 53 °C after 63.33 min with 0.1 g of adsorbent and 8 × 10 -6  M DR81, 12 × 10 -6  M RHB with composite desirability of 0.99. The predicted values for percentage removal of dyes from the mixture were in good agreement with the experimental values with R 2 > 96% for both the dyes. CCD superimposed RSM confirmed that presence of different dyes in a solution created a competition for the adsorbent sites and hence interaction of dyes was one of the most important factor to be studied to simulate the real effluent. The adsorbent showed remarkable adsorption capacities for both the dyes in the mixture. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Composite Magnetic Nanoparticles (CuFe₂O₄) as a New Microsorbent for Extraction of Rhodamine B from Water Samples.

    Science.gov (United States)

    Roostaie, Ali; Allahnoori, Farzad; Ehteshami, Shokooh

    2017-09-01

    In this work, novel composite magnetic nanoparticles (CuFe2O4) were synthesized based on sol-gel combustion in the laboratory. Next, a simple production method was optimized for the preparation of the copper nanoferrites (CuFe2O4), which are stable in water, magnetically active, and have a high specific area used as sorbent material for organic dye extraction in water solution. CuFe2O4 nanopowders were characterized by field-emission scanning electron microscopy (SEM), FTIR spectroscopy, and energy dispersive X-ray spectroscopy. The size range of the nanoparticles obtained in such conditions was estimated by SEM images to be 35-45 nm. The parameters influencing the extraction of CuFe2O4 nanoparticles, such as desorption solvent, amount of sorbent, desorption time, sample pH, ionic strength, and extraction time, were investigated and optimized. Under the optimum conditions, a linear calibration curve in the range of 0.75-5.00 μg/L with R2 = 0.9996 was obtained. The LOQ (10Sb) and LOD (3Sb) of the method were 0.75 and 0.25 μg/L (n = 3), respectively. The RSD for a water sample spiked with 1 μg/L rhodamine B was 3% (n = 5). The method was applied for the determination of rhodamine B in tap water, dishwashing foam, dishwashing liquid, and shampoo samples. The relative recovery percentages for these samples were in the range of 95-99%.

  18. A novel magnetic poly(aniline-naphthylamine)-based nanocomposite for micro solid phase extraction of rhodamine B

    International Nuclear Information System (INIS)

    Bagheri, Habib; Daliri, Rasoul; Roostaie, Ali

    2013-01-01

    Graphical abstract: -- Highlights: •A Fe 3 O 4 –aniline-naphthylamine nanocomposite was prepared via a simple route. •The magnetic nanocomposite was applied for isolation of RhB from water. •The nanocomposite applicability was compared with other pristine polymers. •The method was applied for the determination of RhB in different samples. -- Abstract: A novel Fe 3 O 4 –poly(aniline-naphthylamine)-based nanocomposite was synthesized by chemical oxidative polymerization process as a magnetic sorbent for micro solid phase extraction. The scanning electron microscopy images of the synthesized nanocomposite revealed that the copolymer posses a porous structure with diameters less than 50 nm. The extraction efficiency of this sorbent was examined by isolation of rhodamine B, a mutagenic and carcinogenic dye, from aquatic media in dispersion mode. Among different synthesized polymers, Fe 3 O 4 /poly(aniline-naphthylamine) nanocomposite showed a prominent efficiency. Parameters including the desorption solvent, amount of sorbent, desorption time, sample pH, ionic strength, extraction time and stirring rate were optimized. Under the optimum condition, a linear spiked calibration curve in the range of 0.35–5.00 μg L −1 with R 2 = 0.9991 was obtained. The limits of detection (3S b ) and limits of quantification (10S b ) of the method were 0.10 μg L −1 and 0.35 μg L −1 (n = 3), respectively. The relative standard deviation for water sample with 0.5 μg L −1 of RhB was 4.2% (n = 5) and the absolute recovery was 92%. The method was applied for the determination of rhodamine B in dishwashing foam, dishwashing liquid, shampoo, pencil, matches tips and eye shadows samples and the relative recovery percentage were in the range of 94–99%

  19. Kinetic and catalytic analysis of mesoporous metal oxides on the oxidation of Rhodamine B

    Science.gov (United States)

    Xaba, Morena S.; Noh, Ji-Hyang; Mokgadi, Keabetswe; Meijboom, Reinout

    2018-05-01

    In this study, we demonstrate the synthesis and catalytic activity of different mesoporous transition metal oxides, silica (SiO2), copper oxide (CuO), chromium oxide (Cr2O3), iron oxide (Fe2O3) cobalt oxide (Co3O4), cerium oxide (CeO2) and nickel oxide (NiO), on the oxidation of a pollutant dye, Rhodamine B (RhB). These metal oxides were synthesized by inverse micelle formation method and characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), adsorption-desorption isotherms (BET) and H2-temperature programmed reduction (TPR). UV-vis spectrophotometry was used to monitor the time-resolved absorbance of RhB at λmax = 554 nm. Mesoporous copper oxide was calcined at different final heating temperatures of 250, 350, 450 and 550 °C, and each mesoporous copper oxide catalyst showed unique physical properties and catalytic behavior. Mesoporous CuO-550 with the smallest characteristic path length δ, proved to be the catalyst of choice for the oxidation of RhB in aqueous media. We observed that the oxidation of RhB in aqueous media is dependent on the crystallite size and characteristic path length of the mesoporous metal oxide. The Langmuir-Hinshelwood model was used to fit the experimental data and to prove that the reaction occurs on the surface of the mesoporous CuO. The thermodynamic parameters, EA, ΔH#, ΔS# and ΔG# were calculated and catalyst recycling and reusability were demonstrated.

  20. WATERLESS DYEING [REVIEW

    Directory of Open Access Journals (Sweden)

    DEVRENT Nalan

    2015-05-01

    Full Text Available The textile industry is believed to be one of the biggest consumers of water. Water consumption and exhaustion in dyeing textile materials in conventional methods is an important environmental problem. The cost of waste water treatment will cause a prominent problem in the future as it does today. Increasing consideration of ecologic consequences of industrial processes as well as legislation enforcing the avoidance of environmental problems have caused a reorientation of thinking and promoted projects for replacement of conventional technologies. One of these new technologies is dyeing in supercritical fluids. Dyeing with supercritical carbon dioxide is a favourable concept considering the value of water as a natural resource and the cost of waste water treatment. This dyeing method offers many advantages over conventional aqueous dyeing: During this dyeing process no water is used, therefore there is no waste water problem, no other chemicals are required; the carbon dioxide can be recycled; the dystuff which is not adsorbed on the substrate can be collected and reused; The necessary energy consumption in this process is relatively lower than is needed to heat water in conventional methods of dyeing. Due to unnecessary of drying process, it helps to save both energy and time; and dyeing cycle is shorter compared with traditional methods. In addition carbon dioxide is non-toxic and non-flammable. Supercritical fluid, supercritical dyeing, disperse dyestuffs, solid-fluid equilibrium

  1. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells.

    Science.gov (United States)

    Tan, Dehong; Bai, Bing; Jiang, Donghua; Shi, Lin; Cheng, Shunchang; Tao, Dongbing; Ji, Shujuan

    2014-03-01

    The cytogenetic toxicity of rhodamine B on root tip cells of Allium cepa was investigated. A. cepa were cultured in water (negative control), 10 ppm methyl methanesulfonate (positive control), and three concentrations of rhodamine B (200, 100, and 50 ppm) for 7 days. Rhodamine B inhibited mitotic activity; increased nuclear anomalies, including micronuclei, nuclear buds, and bridged nuclei; and induced oxidative stress in A. cepa root tissues. Furthermore, a substantial amount of long nucleoplasmic bridges were entangled together, and some nuclei were simultaneously linked to several other nuclei and to nuclear buds with nucleoplasmic bridges in rhodamine B-treated cells. In conclusion, rhodamine B induced cytogenetic effects in A. cepa root tip cells, which suggests that the A. cepa root is an ideal model system for detecting cellular interactions.

  2. Textile Dye Removal from Aqueous Solution using Modified Graphite Waste/Lanthanum/Chitosan Composite

    Science.gov (United States)

    Kusrini, E.; Wicaksono, B.; Yulizar, Y.; Prasetyanto, EA; Gunawan, C.

    2018-03-01

    We investigated various pre-treatment processes of graphite waste using thermal, mechanical and chemical methods. The aim of this work is to study the performance of modified graphite waste/lanthanum/chitosan composite (MG) as adsorbent for textile dye removal from aqueous solution. Effect of graphite waste resources, adsorbent size and lanthanum concentration on the dye removal were studied in batch experiments. Selectivity of MG was also investigated. Pre-heated graphite waste (NMG) was conducted at 80°C for 1 h, followed by mechanical crushing of the resultant graphite to 75 μm particle size, giving adsorption performance of ˜58%, ˜67%, ˜93% and ˜98% of the model dye rhodamine B (concentration determined by UV-vis spectroscopy at 554 nm), methyl orange (464 nm), methylene blue (664 nm) and methyl violet (580 nm), respectively from aqueous solution. For this process, the system required less than ˜5 min for adsorbent material to be completely saturated with the adsorbate. Further chemical modification of the pre-treated graphite waste (MG) with lanthanum (0.01 – V 0.03 M) and chitosan (0.5% w/w) did not improve the performance of dye adsorption. Under comparable experimental conditions, as those of the ‘thermal-mechanical-pre-treated-only’ (NMG), modification of graphite waste (MG) with 0.03 M lanthanum and 0.5% w/w chitosan resulted in ˜14%, ˜47%, ˜72% and ˜85% adsorption of rhodamine B, methyl orange, methylene blue and methyl violet, respectively. Selective adsorption of methylene blue at most to ˜79%, followed by methyl orange, methyl violet and rhodamine B with adsorption efficiency ˜67, ˜38, and ˜9% sequentially using MG with 0.03 M lanthanum and 0.5% w/w chitosan.

  3. Photo-electrochemical solar cells with a SnO/sub 2/-liquid junction sensitized with highly concentrated dyes

    Energy Technology Data Exchange (ETDEWEB)

    Shimura, Michiko; Shakushiro, Kiyoaki; Shimura, Yukio

    1986-09-01

    The sensitization of a SnO/sub 2/-liquid junction cell with highly concentrated dyes was investigated. The dyes used were Crystal Violet, Methyl Violet B, Malachite Green, Pararosaniline, and Rhodamine B. Anomalous or positive photovoltages were obtained in the system when Fe(CN)/sub 6//sup 3 -/ was added. The performance of the photovoltaic cells showed an open-circuit photovoltage, Vsub(oc), of 175 mV, a short-circuit photocurrent, Isub(sc), of 12 ..mu..A, and a fill factor of 0.42. The action spectra resembled the absorption spectra of the aggregated dyes. A D-D mechanism is introduced to explain the anomaly of the photovoltage of the SnO/sub 2/ electrode sensitized with the dyes. This behaviour is relevant to the practical usage of such photo-electrochemical cells and merits further investigation.

  4. Anaerobic azo dye reduction

    NARCIS (Netherlands)

    Zee, van der F.P.

    2002-01-01

    Azo dyes, aromatic moieties linked together by azo (-N=N-) chromophores, represent the largest class of dyes used in textile-processing and other industries. The release of these compounds into the environment is undesirable, not only because of their colour, but also

  5. Extraction of dye

    African Journals Online (AJOL)

    Dyes of natural origins are great for color appreciation as any variation in the concentration of dye, mordant, type of water, soil and climate give variations in ... Grey scale and blue dyed silk were used for color fastness rating. ..... Down to Earth.

  6. Thermodynamics of the Rhodamine B Lactone--Zwitterion Equilibrium.

    Science.gov (United States)

    Hinckley, Daniel A.; Seybold, Paul G.

    1987-01-01

    Discusses the benefits of thermochromic transformations for studying thermodynamic properties. Describes an experiment that uses a commercially available dye, attains equilibrium rapidly, employs a simple, single-beam spectrophotometer, and is suitable for both physical chemistry and introductory chemistry laboratories. (TW)

  7. A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.

    Science.gov (United States)

    Alam, Rabiul; Islam, Abu Saleh Musha; Sasmal, Mihir; Katarkar, Atul; Ali, Mahammad

    2018-05-10

    A new sensor (L3) based on Rhodamine-B-en (2) and 2-(pyridin-2-ylmethoxy)benzaldehyde (1) has been developed for highly sensitive and selective recognition of NO in purely aqueous medium where the reaction of NO with the fluorophore leads to an unusual formation of nitrosohydroxylamine with the selective opening of the spirolactam ring over different cations, anions, amino-acids and other biological species with prominent enhancement in absorption and emission intensities. A large enhancement of fluorescence intensity for NO (11 fold) was observed upon addition of 3 equivalents of NO into the sensor in aqueous HEPES buffer (20 mM) at pH 7.20, μ = 0.05 M NaCl with naked eye detection. The corresponding Kf value was evaluated to be (7.55 ± 2.04) × 104 M-1 from the fluorescence titration plot. Quantum yields of L3 and the [L3 + NO] compound are found to be 0.07 and 0.77, respectively, using Rhodamine-6G as the standard. The LOD for NO was determined by the 3σ method and found to be 83.4 nM. The L3 sensor has low cytotoxicity, and is cell permeable and suitable for in vitro NO sensing. The in vivo compatibility of the sensor was also checked on zebrafish.

  8. Photophysical properties of some xanthylium salts performances under CVL pumping

    International Nuclear Information System (INIS)

    Doizi, D.; Lompre, L.A.; Gazeau, M.C.

    1995-01-01

    We report the photochemical and photophysical performances of some new dyes belonging to the xanthylium salts family. Performances under Copper Vapor Laser (CVL) pumping are described and compared to those of Rhodamine 6G. (author)

  9. Application of Micro-cloud point extraction for spectrophotometric determination of Malachite green, Crystal violet and Rhodamine B in aqueous samples

    Science.gov (United States)

    Ghasemi, Elham; Kaykhaii, Massoud

    2016-07-01

    A novel, green, simple and fast method was developed for spectrophotometric determination of Malachite green, Crystal violet, and Rhodamine B in water samples based on Micro-cloud Point extraction (MCPE) at room temperature. This is the first report on the application of MCPE on dyes. In this method, to reach the cloud point at room temperature, the MCPE procedure was carried out in brine using Triton X-114 as a non-ionic surfactant. The factors influencing the extraction efficiency were investigated and optimized. Under the optimized condition, calibration curves were found to be linear in the concentration range of 0.06-0.60 mg/L, 0.10-0.80 mg/L, and 0.03-0.30 mg/L with the enrichment factors of 29.26, 85.47 and 28.36, respectively for Malachite green, Crystal violet, and Rhodamine B. Limit of detections were between 2.2 and 5.1 μg/L.

  10. A dansyl-rhodamine ratiometric fluorescent probe for Hg2+ based on FRET mechanism.

    Science.gov (United States)

    Xie, Puhui; Guo, Fengqi; Wang, Lingyu; Yang, Sen; Yao, Denghui; Yang, Guoyu

    2015-03-01

    Based on resonance energy transfer (FRET) from dansyl to rhodamine 101, a new fluorescent probe (compound 1) containing rhodamine 101 and a dansyl unit was synthesized for detecting Hg(2+) through ratiometric sensing in DMSO aqueous solutions. This probe shows a fast, reversible and selective response toward Hg(2+) in a wide pH range. Hg(2+) induced ring-opening reactions of the spirolactam rhodamine moiety of 1, leading to the formation of fluorescent derivatives that can serve as the FRET acceptors. Very large stokes shift (220 nm) was observed in this case. About 97-fold increase in fluorescence intensity ratio was observed upon its binding with Hg(2+).

  11. Direct thermal dyes

    Science.gov (United States)

    Ehlinger, Edward

    1990-07-01

    Direct thermal dyes are members of a class of compounds referred to in the imaging industry as color formers or leuco dyes. The oldest members of that class have simple triarylmethane structures, and have been employed for years in various dyeing applications. More complex triarylmethane compounds, such as phthalides and fluorans, are now used in various imaging systems to produce color. Color is derived from all of these compounds via the same mechanism, on a molecular level. That is, an event of activation produces a highly resonating cationic system whose interaction with incident light produces reflected light of a specific color. The activation event in the case of a direct thermal system is the creation of a melt on the paper involving dye and an acidic developer. The three major performance parameters in a thermal system are background color, image density, and image stability. The three major dye physical parameters affecting thermal performance are chemical constituency, purity, and particle size. Those dyes having the best combination of characteristics which can also be manufactured economically dominate the marketplace. Manufacturing high performance dyes for the thermal market involves multi-step, convergent reaction sequences performed on large scale. Intermediates must be manufactured at the right time, and at the right quality to be useful.

  12. Dyes for displays

    Science.gov (United States)

    Claussen, U.

    1984-01-01

    The improvement of contrast and visibility of LCD by two different means was undertaken. The two methods are: (1) development of fluorescent dyes to increase the visibility of fluorescent activated displays (FLAD); and (2) development of dichroic dyes to increase the contrast of displays. This work was done in close cooperation with the electronic industry, where the newly synthesized dyes were tested. The targets for the chemical synthesis were selected with the help of computer model calculations. A marketable range of dyes was developed. Since the interest of the electronic industries concerning FLAD was low, the investigations were stopped. Dichroic dyes, especially black mixtures with good light fastness, order parameter, and solubility in nematic phases were developed. The application of these dyes is restricted to indoor use because of an increase of viscosity below -10 C. Applications on a technical scale, e.g., for the automotive industry, will be possible if the displays work at temperatures down to -40 C. This problem requires a complex optimization of the dye/nematic phase system.

  13. The role of rare earth oxide nanoparticles in suppressing the photobleaching of fluorescent organic dyes

    Science.gov (United States)

    Guha, Anubhav; Basu, Anindita

    2013-03-01

    Organic dyes are widely used for both industrial as well as in scientific applications such as the fluorescent tagging of materials. However the process of photobleaching can rapidly degrade dye fluorescence rendering the material non-functional. Thus exploring novel methods for preventing photobleaching can have widespread benefits. In this work we show that the addition of minute quantities of rare earth (RE) oxide nanoparticles can significantly suppress the photobleaching of dyes. The fluorescence of Rhodamine and AlexaFluor dyes was measured as a function of time with and without the addition of CeO2 and La2O3 nanoparticle additives (two RE oxides that contain an oxygen vacancy based defect structure), as well as with FeO nanoparticles (which has an oxygen excess stoichiometry). We find that the rare earth oxides significantly prolonged the lifetimes of the dyes. The results allow us to develop a model based upon the presence of oxygen vacancies defects that allow the RE oxides to act as oxygen scavengers. This enables the RE oxide particles to effectively remove reactive oxygen free radicals generated in the dye solutions during the photoabsorption process. Current affiliation: Harvard University

  14. Dyeing of Polyester with Disperse Dyes: Part 2. Synthesis and Dyeing Characteristics of Some Azo Disperse Dyes for Polyester Fabrics

    Directory of Open Access Journals (Sweden)

    Alya M. Al-Etaibi

    2016-06-01

    Full Text Available The goal of this study was to utilize carrier for accelerating the rate of dyeing not only to enhance dyeing of polyester fabrics dyed with disperse dyes 3a,b, but also to save energy. Both the color strength expressed as dye uptake and the fastness properties of the dyed fabrics were evaluated.

  15. A volumetric three-dimensional digital light photoactivatable dye display

    Science.gov (United States)

    Patel, Shreya K.; Cao, Jian; Lippert, Alexander R.

    2017-07-01

    Volumetric three-dimensional displays offer spatially accurate representations of images with a 360° view, but have been difficult to implement due to complex fabrication requirements. Herein, a chemically enabled volumetric 3D digital light photoactivatable dye display (3D Light PAD) is reported. The operating principle relies on photoactivatable dyes that become reversibly fluorescent upon illumination with ultraviolet light. Proper tuning of kinetics and emission wavelengths enables the generation of a spatial pattern of fluorescent emission at the intersection of two structured light beams. A first-generation 3D Light PAD was fabricated using the photoactivatable dye N-phenyl spirolactam rhodamine B, a commercial picoprojector, an ultraviolet projector and a custom quartz imaging chamber. The system displays a minimum voxel size of 0.68 mm3, 200 μm resolution and good stability over repeated `on-off' cycles. A range of high-resolution 3D images and animations can be projected, setting the foundation for widely accessible volumetric 3D displays.

  16. A NIR-remote controlled upconverting nanoparticle: an improved tool for living cell dye-labeling

    International Nuclear Information System (INIS)

    Zheng, Bin; Gong, Xiaoqun; Wang, Hanjie; Wang, Sheng; Chang, Jin; Wang, Huiquan; Li, Wei; Tan, Jian

    2015-01-01

    In living cells, due to the selective permeability and complicated cellular environment, the uptake efficiency and fluorescence decay of organic dyes during dye-labeling may be influenced, which may eventually result in poor fluorescent imaging. In this work, a protocol of UCNs@mSiO_2-(FA and Azo) core–shell nanocarriers was designed and prepared successfully. The core–shell nanocarriers were assembled from two parts, including a mesoporous silica shell surface modified by folate (FA) and azobenzene (Azo), and an upconverting nanocrystal (UCN) core. The mesoporous silica shell is used for loading organic dyes and conjugating folate which helps to enhance the cellular uptake of nanocarriers. The UCN core works as a transducer to convert near infrared (NIR) light to local UV and visible light to activate a back-and-forth wagging motion of azobenzene molecules on the surface, while the azobenzene acts as a molecular impeller for propelling the release of organic dyes. The nanocarriers of loading organic dyes can maintain the stability of the fluorescent imaging effect better than free organic dyes. The experimental results show that with the help of the nanoparticle, cell uptake efficiency of the model dyes of rhodamine and 4′, 6-diamidino-2-phenylindole (DAPI) was significantly improved. The release of dyes can only be triggered by NIR light exposure and their quantity is highly dependent on the duration of NIR light exposure, thus realizing NIR-regulated dye release spatiotemporally. Our work may open a novel avenue for precisely controlling UCN-based living cell imaging in biotechnology and diagnostics, as well as studying cell dynamics, cell–cell interactions, and tissue morphogenesis. (paper)

  17. Thiophene-based rhodamine as selectivef luorescence probe for Fe(III) and Al(III) in living cells.

    Science.gov (United States)

    Wang, Kun-Peng; Chen, Ju-Peng; Zhang, Si-Jie; Lei, Yang; Zhong, Hua; Chen, Shaojin; Zhou, Xin-Hong; Hu, Zhi-Qiang

    2017-09-01

    The thiophene-modified rhodamine 6G (GYJ) has been synthesized as a novel chemosensor. The sensor has sufficiently high selectivity and sensitivity for the detection of Fe 3+ and Al 3+ ions (M 3+ ) by fluorescence and ultraviolet spectroscopy with a strong ability for anti-interference performance. The binding ratio of M 3+ -GYJ complex was determined to be 2:1 according to the Job's plot. The binding constants for Fe 3+ and Al 3+ were calculated to be 3.91 × 10 8 and 5.26 × 10 8  M -2 , respectively. All these unique features made it particularly favorable for cellular imaging applications. The obvious fluorescence microscopy experiments demonstrated that the probes could contribute to the detection of Fe 3+ and Al 3+ in related cells and biological organs with satisfying resolution. Graphical abstract GYJ has high selectivity and sensitivity for the detection of Fe(III) and Al(III) with the binding ratio of 2:1.

  18. Adsorption of chromium(VI) and Rhodamine B by surface modified tannery waste: Kinetic, mechanistic and thermodynamic studies

    Energy Technology Data Exchange (ETDEWEB)

    Anandkumar, J. [Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Mandal, B., E-mail: bpmandal@iitg.ernet.in [Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam (India)

    2011-02-28

    In this study, various activation methods have been employed to examine the potential reuse of tannery residual biomass (TRB) obtained from vegetable tanning process for the removal of Cr(VI) and Rhodamine B (RB) from aqueous solution. The maximum BET surface area (10.42 m{sup 2}/g), honey comb pore distribution and uptake of both Cr(VI) and RB were achieved when only 3-fold volume of HCl was used to activate the biomass. The pH and temperature experiment showed that they have considerable impact on the adsorption capacity of the used adsorbent. The presence of other ions (Na{sup +}, Ca{sup 2+} and NH{sub 4}{sup +}) significantly reduces the metal uptake but marginal enhancement in the dye removal was observed when Na{sup +} and NH{sub 4}{sup +} ions were present in the solution. The equilibrium data fitted satisfactorily with the Langmuir model and monolayer sorption capacity obtained as 177-217 and 213-250 mg/g for Cr(VI) and RB at 30-50 deg. C, respectively. The sorption kinetics was found to follow the pseudo-second-order kinetic model. The increase in adsorption capacity for both metal and dye with increase in temperature indicates that the uptake was endothermic in nature. The results indicate that the HCl modified TRB (A-TRB) could be employed as a low cost adsorbent for the removal of both Cr(VI) and RB from the aqueous solution including industrial wastewater.

  19. Separation of toxic rhodamine B from aqueous solution using an efficient low-cost material, Azolla pinnata, by adsorption method.

    Science.gov (United States)

    Kooh, Muhammad Raziq Rahimi; Lim, Linda B L; Lim, Lee Hoon; Dahri, Muhammad Khairud

    2016-02-01

    This study investigated the potential of untreated Azolla pinnata (AP) to remove toxic rhodamine B (RB) dye. The effects of adsorbent dosage, pH, ionic strength, contact time, and concentration were studied. Experiments involving the effects of pH and ionic strength indicated that hydrophobic-hydrophobic interactions might be the dominant force of attraction for the RB-AP adsorption system. The kinetics modelling of the kinetics experiment showed that pseudo-second-order best represented the adsorption process. The Weber-Morris intraparticle diffusion model showed that intraparticle diffusion is not the rate-limiting step, while the Boyd model suggested that film diffusion might be rate-limiting. The adsorption isotherm model, Langmuir, best represented the adsorption process, and the maximum adsorption capacity was predicted to be 72.2 and 199.7 mg g(-1) at 25 and 65 °C, respectively. Thermodynamics study indicates spontaneity, endothermic and physisorption-dominant adsorption process. The adsorbents were regenerated to satisfactory level with distilled water, HNO3 and NaOH. Pre-treatment of adsorbent with oxalic acid, citric acid, NaOH, HCl and phosphoric acid was investigated but the adsorption capacity was less than the untreated AP.

  20. High resolution measurement of light in terrestrial ecosystems using photodegrading dyes.

    Directory of Open Access Journals (Sweden)

    Javier Roales

    Full Text Available Incoming solar radiation is the main determinant of terrestrial ecosystem processes, such as primary production, litter decomposition, or soil mineralization rates. Light in terrestrial ecosystems is spatially and temporally heterogeneous due to the interaction among sunlight angle, cloud cover and tree-canopy structure. To integrate this variability and to know light distribution over time and space, a high number of measurements are needed, but tools to do this are usually expensive and limited. An easy-to-use and inexpensive method that can be used to measure light over time and space is needed. We used two photodegrading fluorescent organic dyes, rhodamine WT (RWT and fluorescein, for the quantification of light. We measured dye photodegradation as the decrease in fluorescence across an irradiance gradient from full sunlight to deep shade. Then, we correlated it to accumulated light measured with PAR quantum sensors and obtained a model for this behavior. Rhodamine WT and fluorescein photodegradation followed an exponential decay curve with respect to accumulated light. Rhodamine WT degraded slower than fluorescein and remained unaltered after exposure to temperature changes. Under controlled conditions, fluorescence of both dyes decreased when temperatures increased, but returned to its initial values after cooling to the pre-heating temperature, indicating no degradation. RWT and fluorescein can be used to measure light under a varying range of light conditions in terrestrial ecosystems. This method is particularly useful to integrate solar radiation over time and to measure light simultaneously at different locations, and might be a better alternative to the expensive and time consuming traditional light measurement methods. The accuracy, low price and ease of this method make it a powerful tool for intensive sampling of large areas and for developing high resolution maps of light in an ecosystem.

  1. Turn-on fluorescent chemosensor for Hg2+ based on multivalent rhodamine ligands

    NARCIS (Netherlands)

    Wang, X.; Iqbal, M.; Huskens, Jurriaan; Verboom, Willem

    2012-01-01

    Rhodamine-based fluorescent chemosensors 1 and 2 exhibit selective fluorescence enhancement to Fe3+ and Hg2+ over other metal ions at 580 nm in CH3CN/H2O (3/1, v/v) solution. Bis(rhodamine) chemosensor 1, under optimized conditions (CH3CN/HEPES buffer (0.02 M, pH = 7.0) (95/5, v/v)), shows a high

  2. A NOVEL RHODAMINE-BASED FLUORESCENCE CHEMOSENSOR CONTAINING POLYETHER FOR MERCURY (II IONS IN AQUEOUS SOLUTION

    Directory of Open Access Journals (Sweden)

    Wenqi Du

    Full Text Available A novel rhodamine-based Hg2+ chemosensor P2 containing polyether was readily synthesized and investigated, which displayed high selectivity and sensitivity for Hg2+. Because of good water-solubility of polyther, the rhodamine-based chemosensor containing polyether can be used in aqueous solution. The sensor responded rapidly to Hg2+ in pure water solutions with a 1:1 stoichiometry. Meanwhile, it indicated excellent adaptability and also the responsiveness.

  3. Use of scandium ionic associates with salicylic- or 2-phenylquinoline-4-carboxylic acid and rhodamine C

    Energy Technology Data Exchange (ETDEWEB)

    Kononenko, L.I.; Bel' tyukova, S V; Drobyazko, V N; Poluehktov, N S [AN Ukrainskoj SSR, Kiev. Inst. Obshchej i Neorganicheskoj Khimii; AN Ukrainskoj SSR, Odessa. Inst. Obshchej i Neorganicheskoj Khimii)

    1975-09-01

    With salicylic or 2-phenylquinoline-4-carboxylic acid and rhodamine C scandium forms ion associations whose benzene solutions are capable of luminescence. Optimum conditions for the formation of complexes and the composition of the complex with the ratio of Sc:acid:rhodamine C = 1:2:1 are established. A possibility of luminescence determination of scandium in the presence of rare earths is shown.

  4. A dye laser with a partial-selective resonator

    Energy Technology Data Exchange (ETDEWEB)

    Makogon, M M; Sukhanov, V B

    1977-04-01

    The possibility of controlling the width and spectral position of the generation line of an organic dye laser (Rhodamine 6Zh) whose resonator represents a combination of selective and non-selective channels is demonstrated. The selective channel entails an unsymmetrically mounted prism with whose angular displacement the spectral width can be changed within broad ranges; the non-selective channel maintains the resonator's quality at a sufficiently high level. An expression is given which makes it possible to determine the generation's spectral width when fixing the prism's angular position. The change in the rearrangement band was studied in relation to the qualities of the selective and non-selective channels as determined by the form of the active medium's amplification contour (a narrowing of the spectrum from 0.15 to 0.0019 nm led to a reduction of the rearrangement area from 38.4 to 28.3 nm).

  5. Improved fructan accumulation in perennial ryegrass transformed with the onion fructosyltransferase genes 1-SST and 6G-FFT

    DEFF Research Database (Denmark)

    Gadegaard, Gitte; Didion, Thomas; Foiling, Marianne

    2008-01-01

    Carbohydrate limitation has been identified as a main cause of inefficient nitrogen use in ruminant animals, which feed mainly on fresh forage, hay and silage. This inefficiency results in suboptimal meat and milk productivity. One important molecular breeding strategy is to improve the nutritional...... value of ryegrass (Lolium perenne) by increasing the fructan content through expression of heterologous fructan biosynthetic genes. We developed perennial ryegrass Lines expressing sucrose:sucrose 1-fructosyltransferase and fructan:fructan 6G-fructosyltransferase genes from onion (Allium cepa) which...... exhibited up to a 3-fold increased fructan content. Further, the high fructan content was stable during the growth period, whereas the fructan content in an elite variety, marketed as a high sugar variety, dropped rapidly after reaching its maximum and subsequently remained low. (c) 2007 Elsevier GmbH. ALL...

  6. Fluorescence lifetime of emitters with broad homogeneous linewidths modified in opal photonic crystals

    DEFF Research Database (Denmark)

    Nikolaev, Ivan S.; Lodahl, Peter; Vos, Willem L.

    2008-01-01

    We have investigated the dynamics of spontaneous emission from dye molecules embedded in opal photonic crystals. Fluorescence lifetimes of Rhodamine 6G (R6G) dye were measured as a function of both optical frequency and crystal lattice parameter of the polystyrene opals. Due to the broad...

  7. Removal of Organic Dyes from Industrial Wastewaters Using UV/H2O2, UV/H2O2/Fe (II, UV/H2O2/Fe (III Processes

    Directory of Open Access Journals (Sweden)

    Nezamaddin Daneshvar

    2007-03-01

    Full Text Available UV/H2O2, UV/H2O2/Fe (II and UV/H2O2/Fe (III processes are very effective in removing pollutants from wastewater and can be used for treatment of dyestuff units wastewaters. In this study, Rhodamine B was used as a typical organic dye. Rhodamine B has found wide applications in wax, leather, and paper industries. The results from this study showed that this dye was degradable in the presence of hydrogen peroxide under UV-C irradiation (30W mercury light and Photo-Fenton process. The dye was resistant to UV irradiation. In the absence of UV irradiation, the decolorization efficiency was very negligible in the presence of hydrogen. The effects of different system variables such as initial dye concentration, duration of UV irradiation, and initial hydrogen peroxide concentration were investigated in the UV/H2O2 process. Investigation of the kinetics of the UV/H2O2 process showed that the semi-log plot of the dye concentration versus time was linear, suggesting a first order reaction. It was found that Rhodamine B decolorization efficiencies in the UV/H2O2/Fe (II and UV/H2O2/Fe (III processes were higher than that in the UV/H2O2 process. Furthermore, a solution containing 20 ppm of Rhodamine B was decolorized in the presence 18 mM of H2O2 under UV irradiation for 15 minutes. It was also found that addition of 0.1 mM Fe(II or Fe(III to the solution containing  20  ppm of the dye and 5 mM H2O2 under UV light  illumination decreased removal time to 10 min.

  8. Rhodamine/Nanodiamond as a System Model for Drug Carrier.

    Science.gov (United States)

    Reina, G; Orlanducci, S; Cairone, C; Tamburri, E; Lenti, S; Cianchetta, I; Rossi, M; Terranova, M L

    2015-02-01

    In this paper we present some strategies that are being developed in our labs towards enabling nanodiamond-based applications for drug delivery. Rhodamine B (RhB) has been choosen as model molecule to study the loading of nanodiamonds with active moieties and the conditions for their controlled release. In order to test the chemical/physical interactions between functionalized detonation nanodiamond (DND) and complex molecules, we prepared and tested different RhB@DND systems, with RhB adsorbed or linked by ionic bonding to the DND surface. The chemical state of the DND surfaces before conjugation with the RhB molecules, and the chemical features of the DND-RhB interactions have been deeply analysed by coupling DND with Au nanoparticles and taking advantage of surface enhanced Raman spectroscopy SERS. The effects due to temperature and pH variations on the process of RhB release from the DND carrier have been also investigated. The amounts of released molecules are consistent with those required for effective drug action in conventional therapeutic applications, and this makes the DND promising nanostructured cargos for drug delivery applications.

  9. A new method for dosing rhodamine B in natural water; Dosage de la rhodamine B dans les eaux naturelles

    Energy Technology Data Exchange (ETDEWEB)

    Marichal, M.; Benoit, R. [Commissariat a l' energie atomique et aux energies alternatives - CEA, Centre de Marcoule, Centre de Production de Plutonium de Marcoule, Service de Protection contre les Radiations (France)

    1961-07-01

    A simple and sensitive method well adapted to hydrological research. The dye is first extracted from the water sample by isoamyl alcohol and then the fluorescence of the alcoholic solution, after excitation by ultraviolet radiation, is measured spectrophotometrically. The sensitivity of the method is about 10{sup -12}, that is, a millionth of a milligram of dye per litre. Reprint of a paper published in 'Chimie Analytique', N. 2, Feb 1962, p. 70-72 [French] Methode simple et sensible convenant bien aux recherches hydrologiques. Le colorant est prealablement extrait de l'echantillon d'eau par l'alcool isoamylique; la fluorescence de la solution alcoolique eclairee en lumiere ultraviolette est ensuite mesuree au spectrophotometre. La sensibilite de la methode est de l'ordre de I0{sup -12}, soit un millionieme de milligramme de colorant par litre. Reproduction d'un article publie dans 'Chimie Analytique', N. 2, Feb 1962, p. 70-72.

  10. Quantitative differentiation of dyes with overlapping one-photon spectra by femtosecond pulse-shaping

    International Nuclear Information System (INIS)

    Tkaczyk, Eric R.; Tkaczyk, Alan H.; Mauring, Koit; Ye, Jing Yong; Baker, James R.; Norris, Theodore B.

    2010-01-01

    We demonstrate that DiI and rhodamine B, which are not easily distinguishable to one-photon measurements, can be differentiated and in fact quantified in mixture via tailored two-photon excitation pulses found by a genetic algorithm (GA). A nearly three-fold difference in the ratio of two-photon fluorescence of the two dyes is achieved, without a drop in signal of the favored fluorophore. Implementing an acousto-optic interferometer, we were able to prove that the mechanism of discrimination is second-harmonic tuning by the phase-shaped pulses to the relative maxima and minima of these cross-sections.

  11. Dye gain gold NW array of surface plasmon polariton waveguide

    Directory of Open Access Journals (Sweden)

    Jun Zhu

    Full Text Available Plasmon lasers can support ultrasmall mode confinement and ultrafast dynamics with device feature sizes below the diffraction limit. At present in the single visible light frequency, the optical gain method of constraint SPP on metal nanowires structure reported less. We design the gold nanowire array structure, consisting of PMMA and R6G dye molecules as gain, by 488 nm pump in the middle of the nanowires position for wide range of light, use symmetry broken overcome that momentum does not match the photonic and SPP energy conversion. Theoretical analysis shows that dyes provide coherent optical feedback, resulting in nanowires face will observe laser properties of surface plasmons. Feature analysis: the incident light and pump joint strength is greater than the sum of strength which is the incident light, pump respectively. Under the effect of dye molecules gain effective, length of SPP transmission can increase 1 µm. The results achieved in a single optical frequency of stimulated radiation, application of dye optical gain can achieve continuous gain effect. This is for the future development of plasma amplifier and the wavelength laser. Keywords: SPP, Stimulated radiation, Gold nanowires array, Dye molecules

  12. Hyper-Rayleigh scattering and hyper-Raman scattering of dye-adsorbed silver nanoparticles induced by a focused continuous-wave near-infrared laser

    International Nuclear Information System (INIS)

    Itoh, Tamitake; Ozaki, Yukihiro; Yoshikawa, Hiroyuki; Ihama, Takashi; Masuhara, Hiroshi

    2006-01-01

    We report that hyper-Rayleigh scattering, surface-enhanced hyper-Raman scattering, and two-photon excited luminescence occur intermittently by focusing a continuous-wave near-infrared (cw-NIR) laser into a colloidal silver solution including rhodamine 6G (R6G) and sodium chloride (NaCl). On the other hand, continuous hyper-Rayleigh scattering is observed from colloidal silver free from R6G and NaCl, demonstrating that hyper-Raman scattering and two-photon excited luminescence are attributed to R6G and their intermittent features are dependent on the colloidal dispersion. These results suggest that the cw-NIR laser has three roles; the source of the nonlinear response, optical trapping of nanoparticles, and making nanoparticle aggregates possessing the high activity for the nonlinear response

  13. The ICE-6G_C (VM5a) Global Model of the GIA Process: Antarctica at High Spatial Resolution

    Science.gov (United States)

    Peltier, W. R.; Drummond, R.; Argus, D. F.

    2016-12-01

    The ICE-6G_C (VM5a) global model of the glacial isostatic adjustment process (Argus et al., 2014 GJI 198, 537-563; Peltier et al. , 2015, JGR 119, doi:10.1002/2014JB011176) is the latest model in the ICE-nG (VMx) sequence. The model continues to be unique in that it is the only model whose properties are made freely available at each iterative step in its development. This latest version, which embodies detailed descriptions of the Laurentide , Fennoscandian/Barents Sea, Greenland and Antarctic ice sheets through the most recent glacial cycle, is a refinement based primarily upon the incorporation of the constraints being provided by GPS measurements of the vertical and horizontal motion of the crust as well as GRACE observations of the time dependent gravity field. The model has been shown to provide exceptionally accurate predictions of these space geodetic observations of the response to the most recent Late Quaternary glacial cycle. Particular attention has been paid to the Antarctic component as it is well known on the basis of analyses of the sedimentary stratigraphy off-shore and geomorphological characteristics of the continental shelf, that the Last Glacial Maximum state of the southern continent was one in which grounded ice extended out to the shelf break in most locations, including significant fractions of the Ross Sea and Weddell Sea embayments. In the latter regions especially, it is expected that grounded ice would have existed below sea level. In ICE-6G_C (VM5a) a grounding line tracking algorithm was employed (Stuhne and Peltier, 2015 JGR 120, 1841-1865) in order to describe the unloading of the solid surface by ice that was initially grounded below sea level, an apparently unique characteristic of this model. In the initially published version, in which the Sea Level Equation (SLE) was inverted on a basis of spherical harmonics truncated at degree and order 256, this led to "ringing" in the embayments when the Stokes coefficients of the model

  14. Physiological and transcriptional approaches reveal connection between nitrogen and manganese cycles in Shewanella algae C6G3

    Science.gov (United States)

    Aigle, Axel; Bonin, Patricia; Iobbi-Nivol, Chantal; Méjean, Vincent; Michotey, Valérie

    2017-03-01

    To explain anaerobic nitrite/nitrate production at the expense of ammonium mediated by manganese oxide (Mn(IV)) in sediment, nitrate and manganese respirations were investigated in a strain (Shewanella algae C6G3) presenting these features. In contrast to S. oneidensis MR-1, a biotic transitory nitrite accumulation at the expense of ammonium was observed in S. algae during anaerobic growth with Mn(IV) under condition of limiting electron acceptor, concomitantly, with a higher electron donor stoichiometry than expected. This low and reproducible transitory accumulation is the result of production and consumption since the strain is able to dissimilative reduce nitrate into ammonium. Nitrite production in Mn(IV) condition is strengthened by comparative expression of the nitrate/nitrite reductase genes (napA, nrfA, nrfA-2), and rates of the nitrate/nitrite reductase activities under Mn(IV), nitrate or fumarate conditions. Compared with S. oneidensis MR-1, S. algae contains additional genes that encode nitrate and nitrite reductases (napA-α and nrfA-2) and an Outer Membrane Cytochrome (OMC)(mtrH). Different patterns of expression of the OMC genes (omcA, mtrF, mtrH and mtrC) were observed depending on the electron acceptor and growth phase. Only gene mtrF-2 (SO1659 homolog) was specifically expressed under the Mn(IV) condition. Nitrate and Mn(IV) respirations seem connected at the physiological and transcriptional levels.

  15. A novel magnetic poly(aniline-naphthylamine)-based nanocomposite for micro solid phase extraction of rhodamine B

    Energy Technology Data Exchange (ETDEWEB)

    Bagheri, Habib, E-mail: bagheri@sharif.edu; Daliri, Rasoul; Roostaie, Ali

    2013-09-10

    Graphical abstract: -- Highlights: •A Fe{sub 3}O{sub 4}–aniline-naphthylamine nanocomposite was prepared via a simple route. •The magnetic nanocomposite was applied for isolation of RhB from water. •The nanocomposite applicability was compared with other pristine polymers. •The method was applied for the determination of RhB in different samples. -- Abstract: A novel Fe{sub 3}O{sub 4}–poly(aniline-naphthylamine)-based nanocomposite was synthesized by chemical oxidative polymerization process as a magnetic sorbent for micro solid phase extraction. The scanning electron microscopy images of the synthesized nanocomposite revealed that the copolymer posses a porous structure with diameters less than 50 nm. The extraction efficiency of this sorbent was examined by isolation of rhodamine B, a mutagenic and carcinogenic dye, from aquatic media in dispersion mode. Among different synthesized polymers, Fe{sub 3}O{sub 4}/poly(aniline-naphthylamine) nanocomposite showed a prominent efficiency. Parameters including the desorption solvent, amount of sorbent, desorption time, sample pH, ionic strength, extraction time and stirring rate were optimized. Under the optimum condition, a linear spiked calibration curve in the range of 0.35–5.00 μg L{sup −1} with R{sup 2} = 0.9991 was obtained. The limits of detection (3S{sub b}) and limits of quantification (10S{sub b}) of the method were 0.10 μg L{sup −1} and 0.35 μg L{sup −1} (n = 3), respectively. The relative standard deviation for water sample with 0.5 μg L{sup −1} of RhB was 4.2% (n = 5) and the absolute recovery was 92%. The method was applied for the determination of rhodamine B in dishwashing foam, dishwashing liquid, shampoo, pencil, matches tips and eye shadows samples and the relative recovery percentage were in the range of 94–99%.

  16. Treatment of dyeing wastewater including reactive dyes (Reactive ...

    African Journals Online (AJOL)

    Fungal growth was not observed at pH 2. Maximum fungal decolourisation ocurred at pH 3 for anionic reactive dyes (RR, RBB, RB) and pH 6 for cationic MB dye. The fungal dye bioremoval was associated with the surface charge of the fungus due to electrostatic interactions. Growing R. arrhizus strain decolourised 100% of ...

  17. Hair cosmetics: dyes.

    Science.gov (United States)

    Guerra-Tapia, A; Gonzalez-Guerra, E

    2014-11-01

    Hair plays a significant role in body image, and its appearance can be changed relatively easily without resort to surgical procedures. Cosmetics and techniques have therefore been used to change hair appearance since time immemorial. The cosmetics industry has developed efficient products that can be used on healthy hair or act on concomitant diseases of the hair and scalp. Dyes embellish the hair by bleaching or coloring it briefly, for temporary periods of longer duration, or permanently, depending on the composition of a dye (oxidative or nonoxidative) and its degree of penetration of the hair shaft. The dermatologist's knowledge of dyes, their use, and their possible side effects (contact eczema, cancer, increased porosity, brittleness) can extend to an understanding of cosmetic resources that also treat hair and scalp conditions. Copyright © 2013 Elsevier España, S.L.U. and AEDV. All rights reserved.

  18. Influence of fluorescent dye on physical-mechanical properties of luting cements for confocal microscopy analysis.

    Science.gov (United States)

    Oliveira, Dayane; Prieto, Lúcia; Araújo, Cíntia; Coppini, Erick; Pereira, Gisele; Paulillo, Luís

    2014-12-01

    To evaluate the influence of a fluorescent dye (rhodamine B) on the physical and mechanical properties of three different luting cements: a conventional adhesive luting cement (RelyX ARC, 3M/ESPE), a self-adhesive luting cement (RelyX U-200, 3M/ESPE), and a self-etching and self-adhesive luting cement (SeT PP, SDI). The cements were mixed with 0.03 wt% rhodamine B, formed into bar-shaped specimens (n = 10), and light cured using an LED curing unit (Radii, SDI) with a radiant exposure of 32 J/cm(2) . The Knoop hardness (KHN), flexural strength (FS), and Young's modulus (YM) analyses were evaluated after storage for 24 h. Outcomes were subjected to two-way ANOVA and Tukey's test (P = 0.05) for multiple comparisons. No significant differences in FS or YM were observed among the tested groups (P ≥ 0.05); the addition of rhodamine B increased the hardness of the luting cements tested. The addition of a fluorescent agent at 0.03 wt% concentration does not negatively affect the physical-mechanical properties of the luting cement polymerization behavior. © 2014 Wiley Periodicals, Inc.

  19. Hair Dye and Hair Relaxers

    Science.gov (United States)

    ... For Consumers Consumer Information by Audience For Women Hair Dye and Hair Relaxers Share Tweet Linkedin Pin it More sharing ... products. If you have a bad reaction to hair dyes and relaxers, you should: Stop using the ...

  20. Metabolism of methoxychlor by the P450-monooxygenase CYP6G1 involved in insecticide resistance of Drosophila melanogaster after expression in cell cultures of Nicotiana tabacum.

    Science.gov (United States)

    Joussen, Nicole; Schuphan, Ingolf; Schmidt, Burkhard

    2010-03-01

    Cytochrome P450 monooxygenase CYP6G1 of Drosophila melanogaster was heterologously expressed in a cell suspension culture of Nicotiana tabacum. This in vitro system was used to study the capability of CYP6G1 to metabolize the insecticide methoxychlor (=1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane, 1) against the background of endogenous enzymes of the corresponding non-transgenic culture. The Cyp6g1-transgenic cell culture metabolized 96% of applied methoxychlor (45.8 microg per assay) within 24 h by demethylation and hydroxylation mainly to trishydroxy and catechol methoxychlor (16 and 17%, resp.). About 34% of the metabolism and the distinct formation of trishydroxy and catechol methoxychlor were due to foreign enzyme CYP6G1. Furthermore, methoxychlor metabolism was inhibited by 43% after simultaneous addition of piperonyl butoxide (458 microg), whereas inhibition in the non-transgenic culture amounted to 92%. Additionally, the rate of glycosylation was reduced in both cultures. These results were supported by the inhibition of the metabolism of the insecticide imidacloprid (6; 20 microg, 24 h) in the Cyp6g1-transgenic culture by 82% in the presence of piperonyl butoxide (200 microg). Due to CYP6G1 being responsible for imidacloprid resistance of Drosophila or being involved in DDT resistance, it is likely that CYP6G1 conveys resistance to methoxychlor (1). Furthermore, treating Drosophila with piperonyl butoxide could weaken the observed resistance phenomena.

  1. Sunlight impelled photocatalytic pursuance of Ag-TiO2-SGO and Pt-TiO2-SGO ternary nanocomposites on rhodamine B degradation

    Science.gov (United States)

    Alamelu, K.; Ali, B. M. Jaffar

    2018-04-01

    We demonstrate a hydrothermal method combined with polyol reduction process for the synthesis of an Ag-TiO2-SGO and Pt-TiO2-SGO ternary nanocomposites in which the Ag, Pt and TiO2 nanoparticles are dispersed on the Sulfonated graphene oxide nanosheets. The structural and optical properties of obtained nanocomposites were characterized by XRD, UV-DRS, Raman, FTIR and Photoluminescence spectroscopy. The nanocomposites shows increased light absorption ability in the visible region due to surface plasmon resonance effect of noble metal. The rate of electron-hole pair recombination was significating reduced for nanocomposites system compare to pure. Also, their Performance for the photocatalytic degradation of Rhodamine B as a model organic pollutant is explored. The results showed that Ag-TiO2-SGO and Pt-TiO2-SGO nanocomposites could degrade 95% of the dye within 90 min, under natural sunlight irradiation. The reaction kinetics of ternary nanocomposites exhibit more than 2.2 fold increased photocatalytic activity compared to pristine TiO2. Sulfonated graphene based ternary photocatalyst are potential candidates for wastewater treatment in real time application, due to this ability degrade cationic and anionic dyes.

  2. Dye filled security seal

    International Nuclear Information System (INIS)

    Wilson, D.C.

    1982-01-01

    A security seal for providing an indication of unauthorized access to a sealed object includes an elongate member to be entwined in the object such that access is denied unless the member is removed. The elongate member has a hollow, pressurizable chamber extending throughout its length that is filled with a permanent dye under greater than atmospheric pressure. Attempts to cut the member and weld it together are revealed when dye flows through a rupture in the chamber wall and stains the outside surface of the member

  3. Evolutionary changes in gene expression, coding sequence and copy-number at the Cyp6g1 locus contribute to resistance to multiple insecticides in Drosophila.

    Directory of Open Access Journals (Sweden)

    Thomas W R Harrop

    Full Text Available Widespread use of insecticides has led to insecticide resistance in many populations of insects. In some populations, resistance has evolved to multiple pesticides. In Drosophila melanogaster, resistance to multiple classes of insecticide is due to the overexpression of a single cytochrome P450 gene, Cyp6g1. Overexpression of Cyp6g1 appears to have evolved in parallel in Drosophila simulans, a sibling species of D. melanogaster, where it is also associated with insecticide resistance. However, it is not known whether the ability of the CYP6G1 enzyme to provide resistance to multiple insecticides evolved recently in D. melanogaster or if this function is present in all Drosophila species. Here we show that duplication of the Cyp6g1 gene occurred at least four times during the evolution of different Drosophila species, and the ability of CYP6G1 to confer resistance to multiple insecticides exists in D. melanogaster and D. simulans but not in Drosophila willistoni or Drosophila virilis. In D. virilis, which has multiple copies of Cyp6g1, one copy confers resistance to DDT and another to nitenpyram, suggesting that the divergence of protein sequence between copies subsequent to the duplication affected the activity of the enzyme. All orthologs tested conferred resistance to one or more insecticides, suggesting that CYP6G1 had the capacity to provide resistance to anthropogenic chemicals before they existed. Finally, we show that expression of Cyp6g1 in the Malpighian tubules, which contributes to DDT resistance in D. melanogaster, is specific to the D. melanogaster-D. simulans lineage. Our results suggest that a combination of gene duplication, regulatory changes and protein coding changes has taken place at the Cyp6g1 locus during evolution and this locus may play a role in providing resistance to different environmental toxins in different Drosophila species.

  4. Removal of textile dyes by carbon nanotubes: A comparison between adsorption and UV assisted photocatalysis

    Science.gov (United States)

    Dutta, Arun Kumar; Ghorai, Uttam Kumar; Chattopadhyay, Kalyan Kumar; Banerjee, Diptonil

    2018-05-01

    Amorphous carbon nanotubes were synthesized using low temperature solid state reaction. The as synthesized a-CNTs were used to remove two different textile dyes, Methyl Orange and Rhodamine B from water. Two ways of removal were followed; i.e. Adsorption and UV assisted catalysis. Adsorption experiment was carried out under various conditions. Analysis of the adsorption data was performed using Langmuir, Freundlich and Temkin models. It has been shown that the as prepared samples can effectively be used as adsorbent of textile dyes. Exposure of visible or UV light can make no significant additional effect to the removal efficiency. The mechanism of the adsorption has been found to be following a pseudo 1st order mechanism with corresponding correlation factor >0.95. Also it has been shown that presence of impurities can drastically kill the performance of the sample. This detail comparative study has been reported for the first time.

  5. Changing M3G/M6G ratios and pharmacodynamics in a cancer patient during long-term morphine treatment

    DEFF Research Database (Denmark)

    Andersen, Gertrud; Christrup, Lona Louring; Sjøgren, Per

    2002-01-01

    A cancer patient receiving long-term oral sustained-release morphine treatment and periodically presenting with unusually high plasma M3G/M6G ratios is described. We found the patient's formation of M6G more unstable and perhaps delayed compared to the formation of M3G. There is no apparent...... explanation for this phenomenon and the high M3G/M6G ratios had no implications for the patient's pain experience or side effects from the morphine treatment....

  6. Postglacial Rebound and Current Ice Loss Estimates from Space Geodesy: The New ICE-6G (VM5a) Global Model

    Science.gov (United States)

    Peltier, W. R.; Argus, D.; Drummond, R.; Moore, A. W.

    2012-12-01

    We compare, on a global basis, estimates of site velocity against predictions of the newly constructed postglacial rebound model ICE-6G (VM5a). This model is fit to observations of North American postglacial rebound thereby demonstrating that the ice sheet at last glacial maximum must have been, relative to ICE-5G,thinner in southern Manitoba, thinner near Yellowknife (northwest Territories), thicker in eastern and southern Quebec, and thicker along the British Columbia-Alberta border. The GPS based estimates of site velocity that we employ are more accurate than were previously available because they are based on GPS estimates of position as a function of time determined by incorporating satellite phase center variations [Desai et al. 2011]. These GPS estimates are constraining postglacial rebound in North America and Europe more tightly than ever before. In particular, given the high density of GPS sites in North America, and the fact that the velocity of the mass center (CM) of Earth is also more tightly constrained, the new model much more strongly constrains both the lateral extent of the proglacial forebulge and the rate at which this peripheral bulge (that was emplaced peripheral to the late Pleistocence Laurentia ice sheet) is presently collapsing. This fact proves to be important to the more accurate inference of the current rate of ice loss from both Greenland and Alaska based upon the time dependent gravity observations being provided by the GRACE satellite system. In West Antarctica we have also been able to significantly revise the previously prevalent ICE-5G deglaciation history so as to enable its predictions to be optimally consistent with GPS site velocities determined by connecting campaign WAGN measurements to those provided by observations from the permanent ANET sites. Ellsworth Land (south of the Antarctic peninsula), is observed to be rising at 6 ±3 mm/yr according to our latest analyses; the Ellsworth mountains themselves are observed to be

  7. THE APPLICATION OF NATURAL ZEOLITE FROM CIAMIS AS TiO2 PHOTOCATALYST SUPPORT FOR RHODAMINE B DYE PHOTODEGRADATION

    Directory of Open Access Journals (Sweden)

    Intan Cahaya Dani

    2016-04-01

    Full Text Available Heavy metals such as nickel and cadmium from the waste of human activities (industry, domestic, can lead the pollution and sediments deposited on the seabed. Water pH changing, can lead to the release (leaching metals in the sediment into the water body and then it will be bioaccumulated on biota arround the environment. To see the effect of pH changing on the release (leaching of these metals, extracting the sediment at pH variations has done (TCLP method. From the results of detection metals cadmium (Cd and nickel (Ni release studies, to see the hazards of cadmium and nickel metal, carried out a simulation of bioaccumulation test on biota using bioindikator Cyprinus carpio (OECD Guideline 305. Based on the analysis of data obtained in the nickel content in the sediment extract pH 3, 5 and 7 reached 2.55 to 27.94 µg/g, while for cadmium reaches 4.31 to 4.68 µg/g. Observation of metallic nickel and cadmium bioaccumulation in fish hass done for 28 days by looking at levels of cadmium and nickel on the gills of fish and meat. In the flesh of fish, the highest cadmium concentration of 3.179 µg/g while in the gills is 5.392 µg/g. The highest nickel concentrations in fish flesh is equal to 4.557 µg/g while for gill is equal to 10.417 µg/g. The study results indicate the presence of cadmium and nickel metal accumulation on biota.   Keywords: TCLP method, biota, Cyprinus carpio

  8. OPTIMIZATION OF DYEING PARAMETERS TO DYE COTTON WITH CARROT EXTRACTION

    Directory of Open Access Journals (Sweden)

    MIRALLES Verónica

    2017-05-01

    Full Text Available Natural dyes derived from flora and fauna are believed to be safe because of non-toxic, non-carcinogenic and biodegradable nature. Furthermore, natural dyes do not cause pollution and waste water problems. Natural dyes as well as synthetic dyes need the optimum parameters to get a good dyeing. On some occasions, It is necessary the use of mordants to increase the affinity between cellulose fiber and natural dye, but there are other conditions to optimize in the dyeing process, like time, temperature, auxiliary porducts, etc. In addition, the optimum conditions are different depends on the type of dye and the fiber nature. The aim of this work is the use of carrot extract to dye cotton fabric by exhaustion at diverse dyeing conditions. Diffferent dyeing processes were carried out to study the effect of pH condition and the temperature, using 7, 6 and 4 pH values and 95 ºC and 130ºC for an hour. As a result some images of dyed samples are shown. Moreover, to evaluate the colour of each sample CIELAB parameters are analysed obtained by reflexion spectrophotometre. The results showed that the temperature used has an important influence on the colour of the dyed sample.

  9. Microfluidic Dye Lasers

    DEFF Research Database (Denmark)

    Kristensen, Anders; Balslev, Søren; Gersborg-Hansen, Morten

    2006-01-01

    A technology for miniaturized, polymer based lasers, suitable for integration with planar waveguides and microfluidic networks is presented. The microfluidic dye laser device consists of a microfluidic channel with an embedded optical resonator. The devices are fabricated in a thin polymer film...

  10. (MTT) dye reduction assay.

    African Journals Online (AJOL)

    to inhibit proliferation of HeLa cells was determined using the 3443- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) dye reduction assay. Extracts from roots of Agathisanthemum bojeri, Synaptolepis kirkii and Zanha africana and the leaf extract of Physalis peruviana at a concentration of 10 pg/ml inhibited cell ...

  11. Dye laser principles with applications

    CERN Document Server

    Duarte, Frank J; Liao, Peter F; Kelley, Paul

    1990-01-01

    A tutorial introduction to the field of dye lasers, Dye Laser Principles also serves as an up-to-date overview for those using dye lasers as research and industrial tools. A number of the issues discussed in this book are pertinent not only to dye lasers but also to lasers in general. Most of the chapters in the book contain problem sets that expand on the material covered in the chapter.Key Features* Dye lasers are among the most versatile and successful laser sources currently available in use Offering both pulsed and continuous-wave operation and tunable from the near ultraviole

  12. Extraction/fluorometric determination of uranium with rhodamine B and 2-thenoyltrifluoroacetone

    International Nuclear Information System (INIS)

    Naganuma, Takeshi; Tuzuki, Satoshi; Jin, Jiye.

    1990-01-01

    A method for the fluorometric determination of uranium, based on a solvent extraction of an ion associate formed between Rhodamine B cation and 2-thenoyltrifluoroacetone(TTA)-metal chelate anion with benzene, is described. The procedure for the construction of the calibration curve is as follows. Varying amounts of uranium standard solution and 2 ml of 2 M ammonium acetate buffer solution are mixed. The solution is adjusted at pH 6.0 and 10 ml of 0.3 % Rhodamine B are added. It is then shaken for 5 min with 10 ml of 0.01 M TTA benzene solution. The fluoresence of the organic phase is measured at 545 nm (excitation) and 570 nm (emission) for uranyl-TTA-Rhodamine B complex. The fluorescence intensity is linearly related to the concentration of uranyl ion in the range of 0.05 ppm ot 1 ppm. (author)

  13. A turn-on fluorescent rhodamine-acyl hydrazide for selective detection of Cu"2"+ ions

    International Nuclear Information System (INIS)

    Yoon, Jung Won; Jeong, Hyuk; Lee, Min Hee

    2017-01-01

    We present a rhodamine-based probe that can detect Cu"2"+ ions via Cu"2"+-promoted hydrolysis in aqueous solutions. In solution, the probe is non-fluorescent and colorless, but gives a strong fluorescence at 586 nm and shows a color change to pink on the addition of Cu"2"+ ions. We demonstrate that the rhodamine-based probe undergoes Cu"2"+-promoted hydrolysis with a moderate reaction time (within 3 min) and provides a fluorescence off–on change, even in the presence of other competitive metal ions. The rhodamine-based probe shows a linear correlation between increasing fluorescence at 586 nm and the Cu"2"+ concentration, and can detect Cu"2"+ at nanomolar levels (10 nM) in CH_3CN:H_2O (v/v, 90:10)

  14. Rhodamine B conjugates of triterpenoic acids are cytotoxic mitocans even at nanomolar concentrations.

    Science.gov (United States)

    Sommerwerk, Sven; Heller, Lucie; Kerzig, Christoph; Kramell, Annemarie E; Csuk, René

    2017-02-15

    Triterpenoic acids 1-6 exhibited very low or no cytotoxicity at all, but their corresponding 2,3-di-O-acetyl-piperazinyl amides 13-18 showed low EC 50 values for several human tumor cell lines. Their cytotoxicity, however, was also high for the non-malignant mouse fibroblasts NIH 3T3. A significant improvement was achieved by preparing the rhodamine B derivatives 19-24. While rhodamine B is not cytotoxic (up to a concentration of 30μM - cut-off of the assay), the triterpenoid piperazine-spacered rhodamine B derivatives were cytotoxic in nano-molar concentration. Compound 24 (a diacetylated maslinic acid derivative) was most toxic for several human tumor cell lines but less toxic for mouse fibroblasts NIH 3T3. Staining and double-staining experiments revealed 24 to act as a mitocan. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  15. A rhodamine B-based fluorescent sensor toward highly selective mercury (II) ions detection.

    Science.gov (United States)

    Jiao, Yang; Zhang, Lei; Zhou, Peng

    2016-04-01

    This work presented the design, syntheses and photophysical properties of a rhodamine B-based fluorescence probe, which exhibited a sensitive and selective recognition towards mercury (II). The chemosensor RA (Rhodamine- amide- derivative) contained a 5-aminoisophthalic acid diethyl ester and a rhodamine group, and the property of spirolactone of this chemosensor RA was detected by X-ray crystal structure analyses. Chemosensor RA afforded turn-on fluorescence enhancement and displayed high brightness for Hg(2+), which leaded to the opening of the spirolactone ring and consequently caused the appearance of strong absorption at visible range, moreover, the obvious and characteristic color changed from colorless to pink was observed. We envisioned that the chemosensor RA exhibited a considerable specificity with two mercury (II) ions which was attributed to the open of spirolactone over other interference metal ions. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Degradation of TiO2 and/or SiO2 hybrid films doped with different cationic dyes

    International Nuclear Information System (INIS)

    Purcar, Violeta; Caprarescu, Simona; Donescu, Dan; Petcu, Cristian; Stamatin, Ioan; Ianchis, Raluca; Stroescu, Hermine

    2013-01-01

    Hybrid thin films, silica–titanium oxides and silica–aluminum oxides, designed based on the sol–gel process are evaluated as catalysts in the photo-degradation of the cationic dyes. Silica matrices from different precursors with various organic functional groups and cross-linked with titanium or aluminum agents (tetraisopropyl orthotitanate and aluminum sec-butoxide) allow the surface property tailoring related to the high capacity of the dye adsorption respective, high photo-degradation activity. The cationic dyes (methylene blue, rhodamine B, crystal violet, malachite green) embedded on the hybrid silica matrix, under ultraviolet light, have a first order kinetics of photodegradation. The cross-linking agents play a key role in the photocatalytic degradation and silica matrix as dye absorbent. The photo-degradation rate for the binary system derived from methyltriethoxysilane/vinyltriethoxysilane precursors with both cross linkers showed a significant improvement by comparison with other hybrid materials. The significant increasing in the photodecomposition rate is related to the capacity to generate additional oxidizing species by each silica hybrid compounds. - Highlights: ► Dyes display different electrostatic interactions to the silica matrix. ► Cross-linking agent influences the photocatalytic degradation of dyes. ► Photodegradation reaction obeyed the rules of a pseudo-first-order kinetic reaction. ► UV radiation can be the origin of the photodegradation

  17. Visible-Light-Driven, Dye-Sensitized TiO2 Photo-Catalyst for Self-Cleaning Cotton Fabrics

    Directory of Open Access Journals (Sweden)

    Ishaq Ahmad

    2017-11-01

    Full Text Available We report here the photo-catalytic properties of dye-sensitized TiO2-coated cotton fabrics. In this study, visible-light-driven, self-cleaning cotton fabrics were developed by coating the cotton fabrics with dye-sensitized TiO2. TiO2 nano-sol was prepared via the sol-gel method and the cotton fabric was coated with this nano-sol by the dip-pad–dry-cure method. In order to enhance the photo-catalytic properties of this TiO2-coated cotton fabric under visible light irradiation, the TiO2-coated cotton fabric was dyed with a phthalocyanine-based reactive dye, C.I. Reactive Blue 25 (RB-25, as a dye sensitizer for TiO2. The photo-catalytic self-cleaning efficiency of the resulting dye/TiO2-coated cotton fabrics was evaluated by degradation of Rhodamine B (RhB and color co-ordinate measurements. Dye/TiO2-coated cotton fabrics show very good photo-catalytic properties under visible light.

  18. Gold nanoworms immobilized graphene oxide polymer brush nanohybrid for catalytic degradation studies of organic dyes

    International Nuclear Information System (INIS)

    Mogha, Navin Kumar; Gosain, Saransh; Masram, Dhanraj T.

    2017-01-01

    Highlights: • AuNPs on PDMAEMA brushes immobilized reduced graphene oxide was used as catalyst. • A novel highly efficient, reusable heterogeneous catalyst for dyes degradation. • Rhodamine B, Methyl Orange and Eosin Y was used for study. • Apparent rate constant observed was 21.8, 26.2, and 8.7 (×10 −3 s −1 ) respectively. - Abstract: In the present work, we report gold nanoparticles (AuNPs) on poly (dimethylaminoethyl methacrylate) (PDMAEMA) brushes immobilized reduced graphene oxide (Au/PDMAEMA/RGO) as catalyst for degradation kinetic studies of Rhodamine B (RB), Methyl Orange (MO) and Eosine Y (EY) dyes, having an excellent catalytic activity, as evident by the apparent rate constant (k app ), which is found to be 21.8, 26.2, and 8.7 (×10 −3 s −1 ), for RB, MO and EY respectively. Au/PDMAEMA/RGO catalyst is easy to use, highly efficient, recyclable, which make it suitable for applications in waste water management. Foremost, synthesis of PDMAEMA brushes on graphene oxide is accomplished by Atom transfer radical polymerization method (ATRP), whereas AuNPs are synthesized by simple chemical reduction method.

  19. Gold nanoworms immobilized graphene oxide polymer brush nanohybrid for catalytic degradation studies of organic dyes

    Energy Technology Data Exchange (ETDEWEB)

    Mogha, Navin Kumar; Gosain, Saransh; Masram, Dhanraj T., E-mail: dhnaraj_masram27@rediffmail.com

    2017-02-28

    Highlights: • AuNPs on PDMAEMA brushes immobilized reduced graphene oxide was used as catalyst. • A novel highly efficient, reusable heterogeneous catalyst for dyes degradation. • Rhodamine B, Methyl Orange and Eosin Y was used for study. • Apparent rate constant observed was 21.8, 26.2, and 8.7 (×10{sup −3} s{sup −1}) respectively. - Abstract: In the present work, we report gold nanoparticles (AuNPs) on poly (dimethylaminoethyl methacrylate) (PDMAEMA) brushes immobilized reduced graphene oxide (Au/PDMAEMA/RGO) as catalyst for degradation kinetic studies of Rhodamine B (RB), Methyl Orange (MO) and Eosine Y (EY) dyes, having an excellent catalytic activity, as evident by the apparent rate constant (k{sub app}), which is found to be 21.8, 26.2, and 8.7 (×10{sup −3} s{sup −1}), for RB, MO and EY respectively. Au/PDMAEMA/RGO catalyst is easy to use, highly efficient, recyclable, which make it suitable for applications in waste water management. Foremost, synthesis of PDMAEMA brushes on graphene oxide is accomplished by Atom transfer radical polymerization method (ATRP), whereas AuNPs are synthesized by simple chemical reduction method.

  20. Preparation of a magnetic molecularly imprinted polymer for selective recognition of rhodamine B

    International Nuclear Information System (INIS)

    Liu, Xiuying; Yu, Dan; Yu, Yingchao; Ji, Shujuan

    2014-01-01

    Graphical abstract: A novel material based on the use of magnetic Fe3O4 nanoparticles coated with MMIP for preconcentration and determination of RhB in real samples prior to fluorospectrophotometry was developed. - Highlights: • A novel rhodamine B magnetic molecularly imprinted polymer by using Fe 3 O 4 magnetite as the magnetically susceptible component was synthesized. • The MMIP had rapid adsorption and high selectivity towards rhodamine B. • Rhodamine B can be extracted selectively by MMIP from real samples. • The method provides the advantages of short analysis time and high sensitivity. - Abstract: A novel magnetic molecularly imprinted polymer (MMIP) was developed as an adsorbent to selectively remove rhodamine B from real samples. The polymer was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermo-gravimetric analysis. Static adsorption, kinetic adsorption, and selective recognition experiments were also performed to investigate the specific adsorption equilibrium, kinetics, and selective recognition ability of the MMIP. The MMIPs had outstanding thermal stability, large adsorption capacity, and high competitive selectivity. When they were used as dispersed solid-phase extraction adsorbents in real samples, rhodamine B recovery was 79.97–81.88% and 75.56–79.74% in intra-day and inter-day reproducibility experiments with relative standard deviations lower than 2.62% and 4.28%, respectively. Extraction was optimized for yield and efficiency. Precision, accuracy, and linear working range were determined under optimal experimental conditions. The limits of detection and quantification were 1.05 and 3.49 μg L −1 , respectively. These results suggest MMIPs may be used for determination of rhodamine B in real samples

  1. A Dansyl-Rhodamine Based Fluorescent Probe for Detection of Hg2+ and Cu2.

    Science.gov (United States)

    Yuan, Shizhuang; Su, Wei; Wang, Enju

    2017-09-01

    A novel fluorescent probe based on dansyl-appended rhodamine B was developed. The probe can selectively recognize and sense Hg2+ and Cu2+ from other common metal ions by showing unique fluorescence and absorption characteristics. In MeCN/HEPES buffer solution, the probe gives a ratiometric fluorescent response to Hg2+, which was ascribed to the fluorescence resonance energy transfer from dansyl moiety to the ring-opened rhodamine B moiety, while the presence of Cu2+ causes fluorescence quenching. Beside the fluorescence change, the presence of Cu2+ and Hg2+ can induce intensive absorption at about 555 nm, which resulted in a color change from colorless to pink.

  2. Characteristics of dye-sensitized solar cells using natural dye

    Energy Technology Data Exchange (ETDEWEB)

    Furukawa, Shoji, E-mail: furukawa@cse.kyutech.ac.j [Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka-shi, Fukuoka-ken 820-8502 (Japan); Iino, Hiroshi; Iwamoto, Tomohisa; Kukita, Koudai; Yamauchi, Shoji [Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka-shi, Fukuoka-ken 820-8502 (Japan)

    2009-11-30

    Dye-sensitized solar cells are expected to be used for future clean energy. Recently, most of the researchers in this field use Ruthenium complex as dye in the dye-sensitized solar cells. However, Ruthenium is a rare metal, so the cost of the Ruthenium complex is very high. In this paper, various dye-sensitized solar cells have been fabricated using natural dye, such as the dye of red-cabbage, curcumin, and red-perilla. As a result, it was found that the conversion efficiency of the solar cell fabricated using the mixture of red-cabbage and curcumin was about 0.6% (light source: halogen lamp), which was larger than that of the solar cells using one kind of dye. It was also found that the conversion efficiency was about 1.0% for the solar cell with the oxide semiconductor film fabricated using polyethylene glycol (PEG) whose molecular weight was 2,000,000 and red-cabbage dye. This indicates that the cost performance (defined by [conversion efficiency]/[cost of dye]) of the latter solar cell (dye: red-cabbage) is larger by more than 50 times than that of the solar cell using Ruthenium complex, even if the effect of the difference between the halogen lamp and the standard light source is taken into account.

  3. Enhanced photocatalytic performance and degradation pathway of Rhodamine B over hierarchical double-shelled zinc nickel oxide hollow sphere heterojunction

    Science.gov (United States)

    Zhang, Ying; Zhou, Jiabin; Cai, Weiquan; Zhou, Jun; Li, Zhen

    2018-02-01

    In this study, hierarchical double-shelled NiO/ZnO hollow spheres heterojunction were prepared by calcination of the metallic organic frameworks (MOFs) as a sacrificial template in air via a one-step solvothermal method. Additionally, the photocatalytic activity of the as-prepared samples for the degradation of Rhodamine B (RhB) under UV-vis light irradiation were also investigated. NiO/ZnO microsphere comprised a core and a shell with unique hierarchically porous structure. The photocatalytic results showed that NiO/ZnO hollow spheres exhibited excellent catalytic activity for RhB degradation, causing complete decomposition of RhB (200 mL of 10 g/L) under UV-vis light irradiation within 3 h. Furthermore, the degradation pathway was proposed on the basis of the intermediates during the photodegradation process using liquid chromatography analysis coupled with mass spectroscopy (LC-MS). The improvement in photocatalytic performance could be attributed to the p-n heterojunction in the NiO/ZnO hollow spheres with hierarchically porous structure and the strong double-shell binding interaction, which enhances adsorption of the dye molecules on the catalyst surface and facilitates the electron/hole transfer within the framework. The degradation mechanism of pollutant is ascribed to the hydroxyl radicals (rad OH), which is the main oxidative species for the photocatalytic degradation of RhB. This work provides a facile and effective approach for the fabrication of porous metal oxides heterojunction with high photocatalytic activity and thus can be potentially used in the environmental purification.

  4. Near-Infrared Phosphorus-Substituted Rhodamine with Emission Wavelength above 700 nm for Bioimaging.

    Science.gov (United States)

    Chai, Xiaoyun; Cui, Xiaoyan; Wang, Baogang; Yang, Fan; Cai, Yi; Wu, Qiuye; Wang, Ting

    2015-11-16

    Phosphorus has been successfully fused into a classic rhodamine framework, in which it replaces the bridging oxygen atom to give a series of phosphorus-substituted rhodamines (PRs). Because of the electron-accepting properties of the phosphorus moiety, which is due to effective σ*-π* interactions and strengthened by the inductivity of phosphine oxide, PR exhibits extraordinary long-wavelength fluorescence emission, elongating to the region above 700 nm, with bathochromic shifts of 140 and 40 nm relative to rhodamine and silicon-substituted rhodamine, respectively. Other advantageous properties of the rhodamine family, including high molar extinction coefficient, considerable quantum efficiency, high water solubility, pH-independent emission, great tolerance to photobleaching, and low cytotoxicity, stay intact in PR. Given these excellent properties, PR is desirable for NIR-fluorescence imaging in vivo. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Adsorption of reactive blue BF-5G dye by soybean hulls: kinetics, equilibrium and influencing factors.

    Science.gov (United States)

    Honorio, Jacqueline Ferandin; Veit, Márcia Teresinha; Gonçalves, Gilberto da Cunha; de Campos, Élvio Antonio; Fagundes-Klen, Márcia Regina

    2016-01-01

    The textile industry is known for the high use of chemicals, such as dyes, and large volumes of effluent that contaminate waters, a fact that has encouraged research and improved treatment techniques. In this study, we used unprocessed soybean hulls for the removal of reactive blue BF-5G dye. The point of zero charge of soybean hulls was 6.76. Regarding the speed of agitation in the adsorption process, the resistance to mass transfer that occurs in the boundary layer was eliminated at 100 rpm. Kinetics showed an experimental amount of dye adsorbed at equilibrium of 57.473 mg g(-1) obtained under the following conditions: dye initial concentration = 400 mg L(-1); diameter of particle = 0.725 mm; dosage = 6 g L(-1); pH 2; 100 rpm; temperature = 30 °C; and duration of 24 hours. The pseudo-second order best showed the dye removal kinetics. The adsorption isotherms performed at different temperatures (20, 30, 40 and 50 °C) showed little variation in the concentration range assessed, being properly adjusted by the Langmuir isotherm model. The maximum capacity of dye adsorption was 72.427 mg g(-1) at 30 °C. Since soybean hull is a low-cost industrial byproduct, it proved to be a potential adsorbent for the removal of the textile dye assessed.

  6. Adsorption of reactive dyes from aqueous solutions by fly ash: Kinetic and equilibrium studies

    International Nuclear Information System (INIS)

    Dizge, N.; Aydiner, C.; Demirbas, E.; Kobya, M.; Kara, S.

    2008-01-01

    Adsorption kinetic and equilibrium studies of three reactive dyes namely, Remazol Brillant Blue (RB), Remazol Red 133 (RR) and Rifacion Yellow HED (RY) from aqueous solutions at various initial dye concentration (100-500 mg/l), pH (2-8), particle size (45-112.5 μm) and temperature (293-323 K) on fly ash (FA) were studied in a batch mode operation. The adsorbent was characterized with using several methods such as SEM, XRD and FTIR. Adsorption of RB reactive dye was found to be pH dependent but both RR and RY reactive dyes were not. The result showed that the amount adsorbed of the reactive dyes increased with increasing initial dye concentration and contact time. Batch kinetic data from experimental investigations on the removal of reactive dyes from aqueous solutions using FA have been well described by external mass transfer and intraparticle diffusion models. It was found that external mass transfer and intraparticle diffusion had rate limiting affects on the removal process. This was attributed to the relatively simple macropore structure of FA particles. The adsorption data fitted well with Langmuir and Freundlich isotherm models. The optimum conditions for removal of the reactive dyes were 100 mg/l initial dye concentration, 0.6 g/100 ml adsorbent dose, temperature of 293 K, 45 μm particle size, pH 6 and agitation speed of 250 rpm, respectively. The values of Langmuir and Freundlich constants were found to increase with increasing temperature in the range 135-180 and 15-34 mg/g for RB, 47-86 and 1.9-3.7 mg/g for RR and 37-61 and 3.0-3.6 mg/g for RY reactive dyes, respectively. Different thermodynamic parameters viz., changes in standard free energy, enthalpy and entropy were evaluated and it was found that the reaction was spontaneous and endothermic in nature

  7. Synthesis of azo pyridone dyes

    Directory of Open Access Journals (Sweden)

    Mijin Dušan Ž.

    2011-01-01

    Full Text Available Over 50% of all colorants which are used nowdays are azo dyes and pigments, and among them arylazo pyridone dyes (and pigments have became of interest in last several decades due to the high molar extinction coefficient, and the medium to high light and wet fastness properties. They find application generally as disperse dyes. The importance of disperse dyes increased in the 1970s and 1980s due to the use of polyester and nylon as the main synthetic fibers. Also, disperse dyes were used rapidly since 1970 in inks for the heat-transfer printing of polyester. The main synthetic route for the preparation of azo dyes is coupling reaction between an aromatic diazo compound and a coupling component. Of all dyes manufactured, about 60% are produced by this reaction. Arylazo pyridone dyes can be prepared from pyridone moiety as a coupling component, where substituent can be on nitrogen, and diazonim salts which can be derived from different substituted anilines or other heterocyclic derivatives. In addition, arylazo dyes containing pyridone ring can be prepared from arylazo diketones or arylazo ketoesters (obtained by coupling β-diketones or β-ketoesters with diazonim salts by condensation with cyanoacetamide. Disazo dyes can be prepared by tetrazotizing a dianiline and coupling it with a pyridone or by diazotizing aniline and coupling it with a dipyridone. Trisazo dyes can be also prepared by diazotizing of aniline and coupling it with a tripyridone or by hexazotizing a trianiline and coupling it with a pyridone. The main goal of this paper is to give a brief review on the synthesis of arylazo pyridone dyes due to the lack of such reviews. In addition, some properties of arylazo pyridone dyes as light fastness and azo-hydrazon tautomerism are disccused.

  8. Gold Decorated Graphene for Rapid Dye Reduction and Efficient Electro Catalytic Oxidation of Ethanol

    Science.gov (United States)

    Siddhardha, R. S.; Kumar v, Lakshman; Kaniyoor, A.; Podila, R.; Kumar, V. S.; Venkataramaniah, K.; Ramaprabhu, S.; Rao, A.; Ramamurthy, S. S.; Clemson University Team; Sri Sathya Sai Institute of Higher Learning Team; IITMadras Team

    2013-03-01

    A well known disadvantage in fabrication of metal-graphene composite is the use of surfactants that strongly adsorb on the surface and reduce the performance of the catalyst. Here, we demonstrate a novel one pot synthesis of gold nanoparticles (AuNPs) by laser ablation of gold strip and simultaneous decoration of these on functionalized graphene derivatives. Not only the impregnation of AuNPs was linker free, but also the synthesis by itself was surfactant free. This resulted in in-situ decoration of pristine AuNPs on functionalized graphene derivatives. These materials were well characterized and tested for catalytic applications pertaining to dye reduction and electrooxidation. The catalytic reduction rates are 1.4 x 102 and 9.4x102 times faster for Rhodamine B and Methylene Blue dyes respectively, compared to earlier reports. The enhanced rate involves synergistic interplay of electronic relay between AuNPs and the dye, also charge transfer between the graphene system and dye. In addition, the onset potential for ethanol oxidation was found to be more negative ~ 100 mV, an indication of its promising application in direct ethanol fuel cells.

  9. Dimerization of Organic Dyes on Luminescent Gold Nanoparticles for Ratiometric pH Sensing.

    Science.gov (United States)

    Sun, Shasha; Ning, Xuhui; Zhang, Greg; Wang, Yen-Chung; Peng, Chuanqi; Zheng, Jie

    2016-02-12

    Synergistic effects arising from the conjugation of organic dyes onto non-luminescent metal nanoparticles (NPs) have greatly broadened their applications in both imaging and sensing. Herein, we report that conjugation of a well-known pH-insensitive dye, tetramethyl-rhodamine (TAMRA), to pH-insensitive luminescent gold nanoparticles (AuNPs) can lead to an ultrasmall nanoindicator that can fluorescently report local pH in a ratiometric way. Such synergy originated from the dimerization of TAMRA on AuNPs, of which geometry was very sensitive to surface charges of the AuNPs and can be reversely modulated through protonation of surrounding glutathione ligands. Not limited to pH-insensitive dyes, this pH-dependent dimerization can also enhance the pH sensitivity of fluorescein, a well-known pH-sensitive dye, within a larger pH range, opening up a new pathway to design ultrasmall fluorescent ratiometric nanoindicators with tunable wavelengths and pH response ranges. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Feasibility of Using Fluorescence Spectrophotometry to Develop a Sensitive Dye Immersion Method for Container Closure Integrity Testing of Prefilled Syringes.

    Science.gov (United States)

    Lu, Xujin; Lloyd, David K; Klohr, Steven E

    2016-01-01

    A feasibility study was conducted for a sensitive and robust dye immersion method for the measurement of container closure integrity of unopened prefilled syringes using fluorescence spectrophotometry as the detection method. A Varian Cary Eclipse spectrofluorometer was used with a custom-made sample holder to position the intact syringe in the sample compartment for fluorescence measurements. Methylene blue solution was initially evaluated as the fluorophore in a syringe with excitation at 607 nm and emission at 682 nm, which generated a limit of detection of 0.05 μg/mL. Further studies were conducted using rhodamine 123, a dye with stronger fluorescence. Using 480 nm excitation and 525 nm emission, the dye in the syringe could be easily detected at levels as low as 0.001 μg/mL. The relative standard deviation for 10 measurements of a sample of 0.005 μg/mL (with repositioning of the syringe after each measurement) was less than 1.1%. A number of operational parameters were optimized, including the photomultiplier tube voltage, excitation, and emission slit widths. The specificity of the testing was challenged by using marketed drug products and a protein sample, which showed no interference to the rhodamine detection. Results obtained from this study demonstrated that using rhodamine 123 for container closure integrity testing with in-situ (in-syringe) fluorescence measurements significantly enhanced the sensitivity and robustness of the testing and effectively overcame limitations of the traditional methylene blue method with visual or UV-visible absorption detection. Ensuring container closure integrity of injectable pharmaceutical products is necessary to maintain quality throughout the shelf life of a sterile drug product. Container closure integrity testing has routinely been used to evaluate closure integrity during product development and production line qualification of prefilled syringes, vials, and devices. However, container closure integrity testing

  11. Application of Ni-Oxide@TiO₂ Core-Shell Structures to Photocatalytic Mixed Dye Degradation, CO Oxidation, and Supercapacitors.

    Science.gov (United States)

    Lee, Seungwon; Lee, Jisuk; Nam, Kyusuk; Shin, Weon Gyu; Sohn, Youngku

    2016-12-20

    Performing diverse application tests on synthesized metal oxides is critical for identifying suitable application areas based on the material performances. In the present study, Ni-oxide@TiO₂ core-shell materials were synthesized and applied to photocatalytic mixed dye (methyl orange + rhodamine + methylene blue) degradation under ultraviolet (UV) and visible lights, CO oxidation, and supercapacitors. Their physicochemical properties were examined by field-emission scanning electron microscopy, X-ray diffraction analysis, Fourier-transform infrared spectroscopy, and UV-visible absorption spectroscopy. It was shown that their performances were highly dependent on the morphology, thermal treatment procedure, and TiO₂ overlayer coating.

  12. Adsorption and transport of cadmium and rhodamine WT in pumice sand columns

    International Nuclear Information System (INIS)

    Pang, L.; Close, M.; Greenfield, H.; Stanton, G.

    2004-01-01

    The transport and attenuation of cadmium (Cd) and rhodamine WT (RWT) in a pumice sand aquifer media was investigated using column experiments to study a scenario of point-source contamination. A pore-water velocity of 1.7-1.8 m/day, which is a typical field groundwater velocity in a pumice sand aquifer system, was applied to triplicate columns. A pulse of a solution containing Cd and RWT, together with the conservative tracer tritiated water ( 3 H 2 O) at pH = 7, was introduced into the columns. Experimental results showed that concentration breakthrough curves (BTCs) of 3 H 2 O were symmetrical and fitted well into an equilibrium model. In contrast, BTCs of Cd and RWT were asymmetrical with significant tailings and fitted well with a two-site adsorption/desorption model. The symmetric 3 H 2 O BTCs suggest that physical non-equilibrium was absent in the experimental system, therefore the asymmetrical BTCs of Cd and RWT were attributed to chemical non-equilibrium. Modelling results showed that, in comparison with 3 H 2 O, Cd was apparently retarded by 101-108 times in pumice sand aquifer media (apparent adsorption coefficient 7.33-9.24 ml/g) and underwent a mass loss of 20-30% that was probably because of precipitation of CdCO 3 . As CdCO 3 is extremely insoluble, Cd precipitation would be irreversible and therefore it would not contribute to the tailing of the Cd BTCs. The experimental results suggest that the adsorption and desorption of Cd in pumice sand aquifer media in hydrodynamic conditions was a kinetic process. Cd desorption rates were two orders-of-magnitude slower than its adsorption rates. This resulted in a prolonged mean residence time for Cd in pumice sand aquifer media, which was 10-12 days in the 18-cm-long columns under a flow velocity of 1.7-1.8 m/day. Since the mean residence time is only indicative for the arrival of the central of mass in a contaminant BTC, the time required for the total disappearance of Cd will be much longer than the mean

  13. A dansyl-rhodamine chemosensor for Fe(III) based on off-on FRET.

    Science.gov (United States)

    Piao, Jingyu; Lv, Jia; Zhou, Xin; Zhao, Tong; Wu, Xue

    2014-07-15

    A novel fluorescent chemosensor bearing a rhodamine and a dansyl moiety was developed for highly selective detection of Fe(3+) based on fluorescence resonance energy transfer (FRET) mechanism. Binding of Fe(3+) to the chemosensor induced spirolactam ring opening in the rhodamine moiety and subsequent off-on FRET from the dansyl energy donor to the rhodamine energy acceptor due to the spectral overlap between the emission of the dansyl moiety and the absorption of the ring opened rhodamine moiety. Job's plot analysis indicated a 1:1 binding stoichiometry between the chemosensor and Fe(3+). The association constant was estimated to be 2.72×10(3) M(-1) according to the Benesi-Hildebrand method. With the feature of easy synthesis, simple structural skeleton and excellent sensing ability, the newly synthesized chemosensor provided the potential for applying as a highly selective fluorescent probe in complex samples containing various competitive metal ions and developing other metal ion chemosensors to fulfill various needs of biological and environmental field. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. Highly luminescent hybrid materials based on smectites with polyethylene glycol modified with rhodamine fluorophore

    Czech Academy of Sciences Publication Activity Database

    Sas, S.; Danko, M.; Bizovská, V.; Lang, Kamil; Bujdák, J.

    2017-01-01

    Roč. 138, MAR (2017), s. 25-33 ISSN 0169-1317 Institutional support: RVO:61388980 Keywords : Luminescent polymers * Rhodamine B * Molecular aggregation * Fluorescence * Optical materials Subject RIV: CA - Inorganic Chemistry OBOR OECD: Inorganic and nuclear chemistry Impact factor: 3.101, year: 2016

  15. A dansyl-rhodamine chemosensor for Fe(III) based on off-on FRET

    Science.gov (United States)

    Piao, Jingyu; Lv, Jia; Zhou, Xin; Zhao, Tong; Wu, Xue

    2014-07-01

    A novel fluorescent chemosensor bearing a rhodamine and a dansyl moiety was developed for highly selective detection of Fe3+ based on fluorescence resonance energy transfer (FRET) mechanism. Binding of Fe3+ to the chemosensor induced spirolactam ring opening in the rhodamine moiety and subsequent off-on FRET from the dansyl energy donor to the rhodamine energy acceptor due to the spectral overlap between the emission of the dansyl moiety and the absorption of the ring opened rhodamine moiety. Job's plot analysis indicated a 1:1 binding stoichiometry between the chemosensor and Fe3+. The association constant was estimated to be 2.72 × 103 M-1 according to the Benesi-Hildebrand method. With the feature of easy synthesis, simple structural skeleton and excellent sensing ability, the newly synthesized chemosensor provided the potential for applying as a highly selective fluorescent probe in complex samples containing various competitive metal ions and developing other metal ion chemosensors to fulfill various needs of biological and environmental field.

  16. KELAINAN PATOLOGI PADA MENCIT DAN TIKUS DISEBABKAN ZAT WARNA RHODAMINE B DAN METANIL YELLOW

    Directory of Open Access Journals (Sweden)

    Iwan T. Budiarso

    2012-09-01

    Full Text Available PATHOLOGICAL LESIONS IN MICE AND RATS CAUSED BY DYESTUFFS RHODAMINE B AND METANIL YELLOW. Rhodamine B and Metanil Yellow are 2 non-edible dyestuffs which are widely used for coloring snacks and drinks in Jakarta. These substances are reportedly toxic for human beings, however no data on acute or chronic intoxications are available so far. Groups of mice and rats were fed with either Rhodamine B or Metanil Yellow. The doses of these dyestuffs varied from 0.5 mg to 1350 mg per kilogram body weight. These animals were dcvided into 3 different experimental groups, respectively acute, subacute and chronic toxicity tests. Clinical signs included discoloration of the skin and its intensity depended upon the concentration of the dyestuffs used. The body weight gain of the test animals were consistently lower than those of the controls. Some animals became agressive and cannibalism occurred. Pathological lesions consisted of unthriftiness, focal liver inflammation, hydronephrosis, hepatoma and lymphoma. Considering the results of the experiments, it is justified to warn that the wide use of Rhodamine B and Metanil Yellow for food coloring might be hazardous for human health.

  17. Double-stranded RNA promotes CTL-independent tumor cytolysis mediated by CD11b+Ly6G+ intratumor myeloid cells through the TICAM-1 signaling pathway

    Science.gov (United States)

    Shime, Hiroaki; Matsumoto, Misako; Seya, Tsukasa

    2017-01-01

    PolyI:C, a synthetic double-stranded RNA analog, acts as an immune-enhancing adjuvant that regresses tumors in cytotoxic T lymphocyte (CTL)-dependent and CTL-independent manner, the latter of which remains largely unknown. Tumors contain CD11b+Ly6G+ cells, known as granulocytic myeloid-derived suppressor cells (G-MDSCs) or tumor-associated neutrophils (TANs) that play a critical role in tumor progression and development. Here, we demonstrate that CD11b+Ly6G+ cells respond to polyI:C and exhibit tumoricidal activity in an EL4 tumor implant model. PolyI:C-induced inhibition of tumor growth was attributed to caspase-8/3 cascade activation in tumor cells that occurred independently of CD8α+/CD103+ dendritic cells (DCs) and CTLs. CD11b+Ly6G+ cells was essential for the antitumor effect because depletion of CD11b+Ly6G+ cells totally abrogated tumor regression and caspase activation after polyI:C treatment. CD11b+Ly6G+ cells that had been activated with polyI:C showed cytotoxicity and inhibited tumor growth through the production of reactive oxygen species (ROS)/reactive nitrogen species (RNS). These responses were abolished in either Toll/interleukin-1 receptor domain-containing adaptor molecule-1 (TICAM-1)−/− or interferon (IFN)-αβ receptor 1 (IFNAR1)−/− mice. Thus, our results suggest that polyI:C activates the TLR3/TICAM-1 and IFNAR signaling pathways in CD11b+Ly6G+ cells in tumors, thereby eliciting their antitumor activity, independent of those in CD8α+/CD103+ DCs that prime CTLs. PMID:27834952

  18. Dye lasers in atomic spectroscopy

    International Nuclear Information System (INIS)

    Lange, W.; Luther, J.; Steudel, A.

    1974-01-01

    The properties of dye lasers which are relevant to atomic spectroscopy are discussed. Several experiments made possible by tunable dye lasers are discussed. Applications of high spectral density dye lasers are covered in areas such as absorption spectroscopy, fluorescence spectroscopy, photoionization and photodetachment, and two- and multi-photon processes. Applications which take advantage of the narrow bandwidth of tunable dye lasers are discussed, including saturation spectroscopy, fluorescence line narrowing, classic absorption and fluorescence spectroscopy, nonoptical detection of optical resonances, heterodyne spectroscopy, and nonlinear coherent resonant phenomena. (26 figures, 180 references) (U.S.)

  19. Environmentally Robust Rhodamine Reporters for Probe-based Cellular Detection of the Cancer-linked Oxidoreductase hNQO1.

    Science.gov (United States)

    Best, Quinn A; Johnson, Amanda E; Prasai, Bijeta; Rouillere, Alexandra; McCarley, Robin L

    2016-01-15

    We successfully synthesized a fluorescent probe capable of detecting the cancer-associated quinoneoxidoreductase isozyme-1 within human cells, based on results from an investigation of the stability of various rhodamines and seminaphthorhodamines toward the biological reductant NADH, present at ∼100-200 μM within cells. While rhodamines are generally known for their chemical stability, we observe that NADH causes significant and sometimes rapid modification of numerous rhodamine analogues, including those oftentimes used in imaging applications. Results from mechanistic studies lead us to rule out a radical-based reduction pathway, suggesting rhodamine reduction by NADH proceeds by a hydride transfer process to yield the reduced leuco form of the rhodamine and oxidized NAD(+). A relationship between the structural features of the rhodamines and their reactivity with NADH is observed. Rhodamines with increased alkylation on the N3- and N6-nitrogens, as well as the xanthene core, react the least with NADH; whereas, nonalkylated variants or analogues with electron-withdrawing substituents have the fastest rates of reaction. These outcomes allowed us to judiciously construct a seminaphthorhodamine-based, turn-on fluorescent probe that is capable of selectively detecting the cancer-associated, NADH-dependent enzyme quinoneoxidoreductase isozyme-1 in human cancer cells, without the issue of NADH-induced deactivation of the seminaphthorhodamine reporter.

  20. Selective Activation of N,N'-Diacyl Rhodamine Pro-fluorophores Paired with Releasing Enzyme, Porcine Liver Esterase (PLE).

    Science.gov (United States)

    Abney, Kristopher K; Ramos-Hunter, Susan J; Romaine, Ian M; Godwin, J Shawn; Sulikowski, Gary A; Weaver, Charles David

    2018-04-21

    This study reports the synthesis and testing of a family of rhodamine pro-fluorophores and an enzyme capable of converting pro-fluorophores to Rhodamine 110. We prepared a library of simple N,N'-diacyl rhodamines and investigated Porcine Liver Esterase (PLE) as an enzyme to activate rhodamine-based pro-fluorophores. A PLE-expressing cell line generated an increase in fluorescence rapidly upon pro-fluorophore addition demonstrating the rhodamine pro-fluorophores are readily taken up and fluorescent upon PLE-mediated release. Rhodamine pro-fluorophore amides trifluoroacetamide (TFAm) and proponamide (PAm) appeared to be the best substrates using a cell-based assay using PLE expressing HEK293. Our pro-fluorophore series showed diffusion into live cells and resisted endogenous hydrolysis. The use of our engineered cell line containing the exogenous enzyme PLE demonstrated the rigorousness of amide masking when compared to cells not containing PLE. This simple and selective pro-fluorophore rhodamine pair with PLE offers the potential to be used in vitro and in vivo fluorescence based assays. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Fast, Highly-Sensitive, and Wide-Dynamic-Range Interdigitated Capacitor Glucose Biosensor Using Solvatochromic Dye-Containing Sensing Membrane.

    Science.gov (United States)

    Khan, Md Rajibur Rahaman; Khalilian, Alireza; Kang, Shin-Won

    2016-02-20

    In this paper, we proposed an interdigitated capacitor (IDC)-based glucose biosensor to measure different concentrations of glucose from 1 μM to 1 M. We studied four different types of solvatochromic dyes: Auramine O, Nile red, Rhodamine B, and Reichardt's dye (R-dye). These dyes were individually incorporated into a polymer [polyvinyl chloride (PVC)] and N,N-Dimethylacetamide (DMAC) solution to make the respective dielectric/sensing materials. To the best of our knowledge, we report for the first time an IDC glucose biosensing system utilizing a solvatochromic-dye-containing sensing membrane. These four dielectric or sensing materials were individually placed into the interdigitated electrode (IDE) by spin coating to make four IDC glucose biosensing elements. The proposed IDC glucose biosensor has a high sensing ability over a wide dynamic range and its sensitivity was about 23.32 mV/decade. It also has fast response and recovery times of approximately 7 s and 5 s, respectively, excellent reproducibility with a standard deviation of approximately 0.023, highly stable sensing performance, and real-time monitoring capabilities. The proposed IDC glucose biosensor was compared with an IDC, potentiometric, FET, and fiber-optic glucose sensor with respect to response time, dynamic range width, sensitivity, and linearity. We observed that the designed IDC glucose biosensor offered excellent performance.

  2. Development of new near-infrared and leuco-dye optical systems for forensic and crime fighting applications

    Science.gov (United States)

    Patonay, Gabor; Strekowski, Lucjan; Salon, Jozef; Medou-Ovono, Martial; Krutak, James J.; Leggitt, Jeffrey; Seubert, Heather; Craig, Rhonda

    2004-12-01

    New chemistry for leuco fluorescin and leuco rhodamine for latent bloodstain and fingerprint detection has been developed in our laboratories. The use of these leuco dyes results in excellent contrast for several hours. The FBI's Evidence Response Team and DNA I unit collaborated with Georgia State University to validate the new fluorescin chemistry for use in the field. In addition, several new NIR dyes have been developed in our laboratories that can be used to detect different chemical residues, e.g., pepper spray, latent fingerprint, latent blood, metal ions, or other trace evidence during crime scene investigations. Proof of principle experiments showed that NIR dyes reacting with such residues can be activated with appropriately filtered semiconductor lasers and LEDs to emit NIR fluorescence that can be observed using optimally filtered night vision intensifiers or pocket scopes, digital cameras, CCD and CMOS cameras, or other NIR detection systems. The main advantage of NIR detection is that the color of the background has very little influence on detection and that there are very few materials that would interfere by exhibiting NIR fluorescence. The use of pocket scopes permits sensitive and convenient detection. Once the residues are located, digital images of the fluorescence can be recorded and samples obtained for further analyses. NIR dyes do not interfere with subsequent follow-up or confirmation methods such as DNA or LC/MS analysis. Near-infrared absorbing dyes will be summarized along with detection mechanisms.

  3. Fast, Highly-Sensitive, and Wide-Dynamic-Range Interdigitated Capacitor Glucose Biosensor Using Solvatochromic Dye-Containing Sensing Membrane

    Directory of Open Access Journals (Sweden)

    Md. Rajibur Rahaman Khan

    2016-02-01

    Full Text Available In this paper, we proposed an interdigitated capacitor (IDC-based glucose biosensor to measure different concentrations of glucose from 1 μM to 1 M. We studied four different types of solvatochromic dyes: Auramine O, Nile red, Rhodamine B, and Reichardt’s dye (R-dye. These dyes were individually incorporated into a polymer [polyvinyl chloride (PVC] and N,N-Dimethylacetamide (DMAC solution to make the respective dielectric/sensing materials. To the best of our knowledge, we report for the first time an IDC glucose biosensing system utilizing a solvatochromic-dye-containing sensing membrane. These four dielectric or sensing materials were individually placed into the interdigitated electrode (IDE by spin coating to make four IDC glucose biosensing elements. The proposed IDC glucose biosensor has a high sensing ability over a wide dynamic range and its sensitivity was about 23.32 mV/decade. It also has fast response and recovery times of approximately 7 s and 5 s, respectively, excellent reproducibility with a standard deviation of approximately 0.023, highly stable sensing performance, and real-time monitoring capabilities. The proposed IDC glucose biosensor was compared with an IDC, potentiometric, FET, and fiber-optic glucose sensor with respect to response time, dynamic range width, sensitivity, and linearity. We observed that the designed IDC glucose biosensor offered excellent performance.

  4. A study of the lasing of dyes under the influence of emission from a copper vapor laser

    Energy Technology Data Exchange (ETDEWEB)

    Danilova, V I; Kopylova, T N; Maier, G V; Masarnovskii, L V; Soldatov, A N; Sukhanov, V B

    1980-01-01

    Intense pulsed sources of coherent emission with a continuously tunable wavelength and a high pulse repetition frequency are necessary for atmospheric optics. The use of rhodamine lasing during pumping by a copper dye laser is the most promising. The goals of this work include using the opportunities for improving the lasing properties of dyes pumped by a copper dye laser, choosing dye mixtures that are optimum with respect to their lasing relation, and studying the influence of the dye on their lasing characteristics in order to obtain the optimum energy parameters in the device that is built using a copper vapor laser and an optical attachment. On the basis of an analysis of the equations that describe multiatomic molecular lasing, it is possible to come to a conclusion on the intermolecular processes that determine the lasing effectiveness: singlet-singlet and triplettriplet overabsorption of lasing emission, intercombination (S-T) and internal conversion, and photoconversion in excited electron states. A large probability of emission from the lower singlet state (a large value of the constant of the velocity of radiative decay) is also necessary.

  5. Dye Sensitized Solar Cells

    Directory of Open Access Journals (Sweden)

    Di Wei

    2010-03-01

    Full Text Available Dye sensitized solar cell (DSSC is the only solar cell that can offer both the flexibility and transparency. Its efficiency is comparable to amorphous silicon solar cells but with a much lower cost. This review not only covers the fundamentals of DSSC but also the related cutting-edge research and its development for industrial applications. Most recent research topics on DSSC, for example, applications of nanostructured TiO2, ZnO electrodes, ionic liquid electrolytes, carbon nanotubes, graphene and solid state DSSC have all been included and discussed.

  6. Dye solar cell research

    CSIR Research Space (South Africa)

    Cummings, F

    2009-11-01

    Full Text Available Cummings Energy and Processes Materials Science and Manufacturing Council for Scientific and Industrial Research P.O. Box 395 Pretoria 0001, South Africa 27 November 2009 CONTENT head2rightBackground head2rightCSIR Dye Solar Cell Research head2... rightCollaborations and Links © CSIR 2007 www.csir.co.za head2rightAcknowledgements BACKGROUND head2rightSA is dry: Annual rainfall average of 450 mm compared with a world average of 860 mm head2rightOn upside, we have some...

  7. CW organic dye laser

    International Nuclear Information System (INIS)

    Tuccio, S.A.; Peterson, O.G.

    1975-01-01

    A method and apparatus for producing continuous emission from a lasing medium comprising organic dye molecules in solution are described. Continuous emission is accomplished by flowing the medium through a focused optical cavity while simultaneously producing a population inversion in that portion of the medium flowing in close proximity to the focal point of the cavity. The population inversion is produced by pumping the medium longitudinally, along the optical axis of the cavity, preferably by the focused output of a continuous-wave argon laser. Sufficient thermal energy is continuously dissipated from the medium to maintain the optical homogeneity thereof at or above the quality required for continuous emission

  8. Warm-White-Light-Emitting Diode Based on a Dye-Loaded Metal-Organic Framework for Fast White-Light Communication.

    Science.gov (United States)

    Wang, Zhiye; Wang, Zi; Lin, Bangjiang; Hu, XueFu; Wei, YunFeng; Zhang, Cankun; An, Bing; Wang, Cheng; Lin, Wenbin

    2017-10-11

    A dye@metal-organic framework (MOF) hybrid was used as a fluorophore in a white-light-emitting diode (WLED) for fast visible-light communication (VLC). The white light was generated from a combination of blue emission of the 9,10-dibenzoate anthracene (DBA) linkers and yellow emission of the encapsulated Rhodamine B molecules. The MOF structure not only prevents dye molecules from aggregation-induced quenching but also efficiently transfers energy to the dye for dual emission. This light-emitting material shows emission lifetimes of 1.8 and 5.3 ns for the blue and yellow components, respectively, which are significantly shorter than the 200 ns lifetime of Y 3 Al 5 O 12 :Ce 3+ in commercial WLEDs. The MOF-WLED device exhibited a modulating frequency of 3.6 MHz for VLC, six times that of commercial WLEDs.

  9. Echinococcus canadensis (Cestoda: Taeniidae) is a valid species consisting of the mitochondrial genotypes G6, G7, G8 and G10

    Science.gov (United States)

    The species status of Echinococcus canadensis has long been controversial, mainly because it consists of the mitochondrial genotypes G6, G7, G8 and G10 with different host affinity: G6 (camel strain) and G7 (pig strain) with domestic cycles and G8 (cervid strain) and G10 (Fennoscandian cervid strain...

  10. Statement of basis/proposed plan for the Central Shops Burning/Rubble Pit (631-6G). Revision 1, Final

    International Nuclear Information System (INIS)

    Palmer, E.

    1996-01-01

    The purpose of this plan is to describe the preferred alternative for addressing the Central Shops Burning/Rubble Pit 631-6G (BRP6G) located at SRS, in northwestern Barnwell County, South Carolina and to provide an opportunity for public input into the remedial action selection process. Arsenic, beryllium, iron, and octachloro-dibenzo-p-dioxin isomers (OCDD) concentrations in the pit soil are at levels consistent with those found in the background. Therefore, the only contamination attributable to actions in BRP6G is PCB-1254. After the risk contributions of these chemicals are eliminated, the only remaining risk attributable to the pit soil is from PCB-1254 (about 2 x 10 -6 via ingestion of vegetables grown on-site). The maximum concentration of PCB-1254 detected in the pit was 0.115 mg/kg, approximately 10% of the residential action level for PCBs of 1 mg/kg. Based on the results of the remedial investigation and the BRA, it is proposed that No Action be performed for the BRP6G. Considering the low levels of residual contamination present principally below 1.2 meters (4 feet) within the pit and the associated risks (about 2 x 10 -6 ) within the lower level of EPA's target risk range, action is not warranted for this unit

  11. RCRA Facility Investigation/Remedial Investigation Report with Baseline Risk Assessment for the Central Shops Burning/Rubble Pit (631-6G), Volume 1 Final

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    The Burning/Rubble Pits at the Savannah River Site were usually shallow excavations approximately 3 to 4 meters in depth. Operations at the pits consisted of collecting waste on a continuous basis and burning on a monthly basis. The Central Shops Burning/Rubble Pit 631- 6G (BRP6G) was constructed in 1951 as an unlined earthen pit in surficial sediments for disposal of paper, lumber, cans and empty galvanized steel drums. The unit may have received other materials such as plastics, rubber, rags, cardboard, oil, degreasers, or drummed solvents. The BRP6G was operated from 1951 until 1955. After disposal activities ceased, the area was covered with soil. Hazardous substances, if present, may have migrated into the surrounding soil and/or groundwater. Because of this possibility, the United States Environmental Protection Agency (EPA) has designated the BRP6G as a Solid Waste Management Unit (SWMU) subject to the Resource Conservation Recovery Act/Comprehensive Environmental Response, Compensation and Liability Act (RCRA/CERCLA) process.

  12. Use of dyes in cariology.

    Science.gov (United States)

    van de Rijke, J W

    1991-04-01

    The property of dyes to enhance contrast by their colour can be used in clinical dentistry and in investigations in vitro or in vivo. They have been used for indication of affected dental tissues, improvement of diagnostic methods, enhancement of patient awareness and information about specific processes. The development of particular dye systems, aimed at clinical application, is often laborious because of toxic effects, lack of specificity, irreversible staining or difficulties with removal of the dye. Clinically used dyes are often visually observed, which means a qualitative assessment of the staining, while quantification of the staining, if performed at all, is confined mostly to laboratory experiments. In this paper the application of dyes, arranged according to their specific purpose in cariology, is discussed, and a brief historical overview is given of the development of two particular dye applications for which commercial dye systems are now available. If certain requirements are met, dyes can be of great help in detection and quantification when used with several diagnostic methods.

  13. Assimilating the ICE-6G_C Reconstruction of the Latest Quaternary Ice Age Cycle Into Numerical Simulations of the Laurentide and Fennoscandian Ice Sheets

    Science.gov (United States)

    Stuhne, G. R.; Peltier, W. R.

    2017-12-01

    We analyze the effects of nudging 100 kyr numerical simulations of the Laurentide and Fennoscandian ice sheets toward the glacial isostatic adjustment-based (GIA-based) ICE-6G_C reconstruction of the most recent ice age cycle. Starting with the ice physics approximations of the PISM ice sheet model and the SeaRISE simulation protocols, we incorporate nudging at characteristic time scales, τf, through anomalous mass balance terms in the ice mass conservation equation. As should be expected, these mass balances exhibit physically unrealistic details arising from pure GIA-based reconstruction geometry when nudging is very strong (τf=20 years for North America), while weakly nudged (τf=1,000 years) solutions deviate from ICE-6G_C sufficiently to degrade its observational fit quality. For reasonable intermediate time scales (τf=100 years and 200 years), we perturbatively analyze nudged ice dynamics as a superposition of "leading-order smoothing" that diffuses ICE-6G_C in a physically and observationally consistent manner and "higher-order" deviations arising, for instance, from biases in the time dependence of surface climate boundary conditions. Based upon the relative deviations between respective nudged simulations in which these biases follow surface temperature from ice cores and eustatic sea level from marine sediment cores, we compute "ice core climate adjustments" that suggest how local paleoclimate observations may be applied to the systematic refinement of ICE-6G_C. Our results are consistent with a growing body of evidence suggesting that the geographical origins of Meltwater Pulse 1B (MWP1b) may lie primarily in North America as opposed to Antarctica (as reconstructed in ICE-6G_C).

  14. Radioactive and dye tracer studies for the NWNT sewerage outfall, Hong Kong, and comparison to near-field modelling

    International Nuclear Information System (INIS)

    Horton, P.R.; Wilson, J.R.

    1997-01-01

    A monitoring programme for the North West New Territories (NWNT) sewage outfall, located in the Urmston Road channel in Hong Kong, was completed in 1996. This included three surveys measuring effluent behaviour and oceanographic conditions near the outfall. Radioisotopes gold-198 (γ energy 0.42 MeV, half-life 2.7 days) and tritium (as tritiated water, HTO, β energy 0.018 MeV, half-life 12.3 years) were used to trace the effluent discharging from the outfall during both a wet season and dry season survey. The effluent was also simultaneously tagged with Rhodamine dye which was detected with fluorometers. The gold-198 was generally traced in real time using submersible detectors, while the tritium was measured in the laboratory from sea water samples using liquid scintillation techniques. The Radioisotopes and dye measurement techniques were progressively refined over the surveys and a reliable equipment arrangement and sampling procedure was established

  15. Hydrothermally synthesized reduced graphene oxide/nickel hydroxide (rGO/Ni(OH)2) nanocomposite: A promising material in dye removal

    Science.gov (United States)

    Debata, Suryakanti; Das, Trupti R.; Madhuri, Rashmi; Sharma, Prashant K.

    2017-05-01

    In order to fulfill the unquenchable demand of this expanded human society, a large number of industries have grown up resulting in a severe pollution in air, soil and water as well. Industrial dye is one of the most abundant contaminant in fresh water. Here we have prepared reduced graphene oxide/nickel hydroxide nanocomposite (rGo/Ni(OH)2) by a facile hydrothermal method, aiming for the treatment of water disposed by the textile industries. The characteristic properties of the prepared sample was observed by field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR). The effect of rGo/Ni(OH)2 on the adsorption of Rhodamine B (Rh-B) in aqueous solution was investigated, mainly focusing on the removal time. It was found that, at 45 min, the composite shows a complete disappearance of the initial concentration of Rhodamine B (RhB).

  16. Removal of Rhodamine B under visible irradiation in the presence of Fe⁰, H₂O₂, citrate and aeration at circumneutral pH.

    Science.gov (United States)

    Hong, Jun; Lu, Sijia; Zhang, Caixiang; Qi, Shihua; Wang, Yanxin

    2011-09-01

    A new Vis-Fe(0)-H(2)O(2)-citrate-O(2) system comprising zero-valent iron, hydrogen peroxide, citrate anion and aeration at circumneutral pH under visible irradiation was studied. 21 μmol L(-1) of Rhodamine B (RhB) was chosen as the substrate to be tested. Experiments were conducted under conditions of 2.9 mmol L(-1) of H(2)O(2), 12.6g of Fe(0) and 1.0 mmol L(-1) of citrate at pH 7.5. Results showed that, in 1h reaction, 54% of RhB was removed with corresponding 26% of COD reduced. Meanwhile, the amount of released dissolved irons from Fe(0) surface was found to be at a very low level as removal in 3h reaction. Control and factor influencing experiments showed that the prohibitive extents of individual factor importance on RhB removal followed a decreasing order of Fe(0)>H(2)O(2)>citrate>Vis>O(2). This study showed an excellent system that could remove refractory organic compounds from water in laboratory researches, and also provided a good idea to reduce secondary contamination by dissolved irons in future investigations. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. Ferrocene-functionalized graphitic carbon nitride as an enhanced heterogeneous catalyst of Fenton reaction for degradation of Rhodamine B under visible light irradiation.

    Science.gov (United States)

    Lin, Kun-Yi Andrew; Lin, Jyun-Ting

    2017-09-01

    To enhance degradation of Rhodamine B (RhB), a toxic xanthene dye, an iron-doped graphitic carbon nitride (CN) is prepared by establishing a covalent bond (-CN-) bridging ferrocene (Fc) and CN via a Schiff base reaction. The π-conjugation between the aromatic Fc and CN can be much enhanced by the covalent bond, thereby facilitating the bulk-to-surface charge transfer and separation as well as reversible photo-redox reactions during photocatalytic reactions. Thus, the resulting Fc-CN exhibits a much higher catalytic activity than CN to activate hydrogen peroxide (HP) for RhB degradation, because the photocatalytically generated electrons from CN can activate HP and effectively maintain the bivalence state of Fe in Fc, which also induces the activation of HP. The RhB degradation by the Fc-CN activated HP process (Fc-CN-HP) is validated to involve OH • by examining the effect of radical probe agent as well as electron paramagnetic resonance (EPR) spectroscopic analysis. Fc-CN is also proven to activate HP for RhB degradation over multiple times without loss of catalytic activity. Through determining the degradation intermediates, RhB is indeed fully decomposed by Fc-CN-HP into much lower-molecular-weight organic compounds. These features indicate that Fc-functionalization can be an advantageous technique to enhance the catalytic activity of CN for activating HP. The results obtained in this study are essential to further design and utilize Fc-functionalized CN for Fenton-like reactions. The findings shown here, especially the degradation mechanism and pathway, are also quite important for treating xanthene dyes in wastewater. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Biosorption of Acid Dye in Single and Multidye Systems onto Sawdust of Locust Bean (Parkia biglobosa Tree

    Directory of Open Access Journals (Sweden)

    Abdur-Rahim Adebisi Giwa

    2016-01-01

    Full Text Available Properties of raw sawdust of Parkia biglobosa, as a biosorbent for the removal of Acid Blue 161 dye in single, binary, and ternary dye systems with Rhodamine B and Methylene Blue dyes in aqueous solution, were investigated. The sawdust was characterized using Scanning Electron Microscopy, Fourier Transform Infrared spectrophotometry, X-ray diffraction, and pH point of zero charge. Batch adsorption experiments were carried out to determine the equilibrium characteristics, thermodynamics, and kinetics of the sorption processes. The data obtained were subjected to various isotherm and kinetics equations. The results showed that the adsorption processes were described by different isotherm models depending on the composition of the system; they were all spontaneous (ΔG ranges from −0.72 to −5.36 kJ/mol and endothermic (range of ΔH is 11.37–26.31 kJ/mol and with increased randomness with ΔS values of 55.55 and 98.78 J·mol/K for single and ternary systems, respectively. Pseudo-second-order kinetics model gave better fit for all the sorption systems studied irrespective of the differences in composition, with the initial and overall rate constants higher for the mixtures than for the single system (6.76 g·mg−1min−1. The presence of Rhodamine B and Methylene Blue had a synergetic effect on the maximum monolayer capacity of the adsorbent for Acid Blue 161 dye.

  19. Broadband Light-Harvesting Molecular Triads with High FRET Efficiency Based on the Coumarin-Rhodamine-BODIPY Platform.

    Science.gov (United States)

    He, Longwei; Zhu, Sasa; Liu, Yong; Xie, Yinan; Xu, Qiuyan; Wei, Haipeng; Lin, Weiying

    2015-08-17

    Broadband capturing and FRET-based light-harvesting molecular triads, CRBs, based on the coumarin-rhodamine-BODIPY platform were rationally designed and synthesized. The absorption band of CRBs starts from blue-green to yellow-orange regions (330-610 nm), covering the strong radiation scope of sunlight. The peripheral coumarin and BODIPY chromophore energy could transfer to the central acceptor rhodamine by a one-step direct way. The energy of the coumarin moiety could also transfer to the BODIPY unit, subsequently transferring to the rhodamine core by two-step sequential ways. Both the efficiencies of the coumarin moiety and the BODIPY unit to the rhodamine core in CRBs, determined by two different ways, are very high. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Chemiluminescence behavior based on oxidation reaction of rhodamine B with cerium(IV) in sulfuric acid medium

    International Nuclear Information System (INIS)

    Ma Yongjun; Jin Xiaoyong; Zhou Min; Zhang Ziyu; Teng Xiulan; Chen Hui

    2003-01-01

    The chemiluminescence (CL) of the rhodamine B (RhB)-cerium(IV) system was investigated by flow-injection. Rhodamine B was suggested to be a suitable chemiluminescent reagent in acidic conditions. When the concentration of rhodamine B was 100 mg l -1 and cerium sulfate was 1.6 mmol l -1 in sulfuric acid, the chemiluminescent intensity was found to be highest by using 0.3 mol l -1 sulfuric acid as a carrier solution. The particular chemiluminescent system could tolerate such distinct acidic environments that it was utilized for detecting many compounds that are stable in acidic solutions. Furthermore, by virtue of IR, UV-Vis and luminescence spectroscopic measurements, the chemiluminescent behavior of rhodamine B was studied and a possible mechanism for this chemiluminescent reaction was proposed. The emitter was affirmed to be a radical species due to one of the oxidation products of RhB; the chemiluminescent emissive wavelength was about 425 nm

  1. Nanocomposite of exfoliated bentonite/g-C3N4/Ag3PO4 for enhanced visible-light photocatalytic decomposition of Rhodamine B.

    Science.gov (United States)

    Ma, Jianfeng; Huang, Daiqin; Zhang, Wenyi; Zou, Jing; Kong, Yong; Zhu, Jianxi; Komarneni, Sridhar

    2016-11-01

    Novel visible-light-driven heterojunction photocatalyst comprising exfoliated bentonite, g-C3N4 and Ag3PO4 (EB/g-C3N4/Ag3PO4) was synthesized by a facile and green method. The composites EB/g-C3N4/Ag3PO4 were characterized by X-ray diffraction, Transmission electron microscopy, Fourier transform infrared spectroscopy, UV-Vis diffuse reflectance spectroscopy and the Brunauer, Emmett, and Teller (BET) surface area method. Under visible light irradiation, EB/g-C3N4/Ag3PO4 composites displayed much higher photocatalytic activity than that of either pure g-C3N4 or pure Ag3PO4 in the degradation of Rhodamine B (RhB). Among the hybrid photocatalysts, EB/g-C3N4/Ag3PO4 composite containing 20 wt% Ag3PO4 exhibited the highest photocatalytic activity for the decolorization of RhB. Under the visible-light irradiation, the RhB dye was completely decolorized in less than 60 min. The enhanced photocatalytic performance is attributed to the stable structure, enlarged surface area, strong adsorbability, strong light absorption ability, and high-efficiency separation rate of photoinduced electron-hole pairs. Our finding paves a way to design highly efficient and stable visible-light-induced photocatalysts for practical applications in wastewater treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Chip-based molecularly imprinted monolithic capillary array columns coated GO/SiO2 for selective extraction and sensitive determination of rhodamine B in chili powder.

    Science.gov (United States)

    Zhai, Haiyun; Huang, Lu; Chen, Zuanguang; Su, Zihao; Yuan, Kaisong; Liang, Guohuan; Pan, Yufang

    2017-01-01

    A novel solid-phase extraction chip embedded with array columns of molecularly imprinted polymer-coated silanized graphene oxide (GO/SiO2-MISPE) was established to detect trace rhodamine B (RB) in chili powder. GO/SiO2-MISPE monolithic columns for RB detection were prepared by optimizing the supporting substrate, template, and polymerizing monomer under mild water bath conditions. Adsorption capacity and specificity, which are critical properties for the application of the GO/SiO2-MISPE monolithic column, were investigated. GO/SiO2-MIP was examined by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy. The recovery and the intraday and interday relative standard deviations for RB ranged from 83.7% to 88.4% and 2.5% to 4.0% and the enrichment factors were higher than 110-fold. The chip-based array columns effectively eliminated impurities in chili powder, indicating that the chip-based GO/SiO2-MISPE method was reliable for RB detection in food samples using high-performance liquid chromatography. Accordingly, this method has direct applications for monitoring potentially harmful dyes in processed food. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Photocatalytic degradation of rhodamine B, paracetamol and diclofenac sodium by supported titania-based catalysts from petrochemical residue: effect of doping with magnesium.

    Science.gov (United States)

    da Silva, William Leonardo; Lansarin, Marla Azário; Dos Santos, João Henrique Z; Silveira, Fernando

    2016-11-01

    Three different lots of a residual Ziegler-Natta catalyst slurry (bearing Ti and Mg) obtained from an industrial petrochemical plant were employed as sources for the photocatalyst supported on silica. The effect of additional magnesium (1.0-25.0 wt% Mg/SiO 2 ) on the photocatalytic properties of the doped materials was investigated. Doping the titania-based photocatalyst with Mg results in a shift in the absorption threshold toward the visible spectrum. The optical band gap energy of the bare supported photocatalyst was in the range of 2.5 eV and shifted to 1.72 eV after 25 wt% Mg doping. The systems were evaluated for the photodegradation of one dye (rhodamine B (RhB)) and two drugs (paracetamol and diclofenac sodium) either under ultraviolet (UV) (365 nm - UVA) or visible radiation, separately. Among the evaluated systems, doping with 25 wt% Mg afforded the highest degradation values for the target molecules under UV and visible radiation (i.e. 87%, 60% and 55% of the RhB, paracetamol and diclofenac under UV, respectively, and 82%, 48.3% and 48% under visible irradiation, respectively).

  4. Dynamics of Intercalation/De-Intercalation of Rhodamine B during the Polymorphic Transformation of CdAl Layered Double Hydroxide to the Brucite-Like Cadmium Hydroxide

    KAUST Repository

    Saliba, Daniel

    2016-06-23

    Cadmium-Aluminum layered double hydroxide (CdAl LDH) is thermodynamically unstable and transforms to Cd(OH)2 and Al(OH)3 in a short period of time. We present a reaction-diffusion framework that enables us to use in situ steady-state fluorescence spectroscopy to study the kinetics of intercalation of a fluorescent probe (Rhodamine B (RhB)) during the formation of the CdAl LDH and its de-intercalation upon the conversion of the LDH phase to the β phase (Cd(OH)2). The method involves the diffusion of sodium hydroxide into a hydrogel gel matrix containing the aluminum and cadmium ions as well as the species we wish to incorporate in the interlayers of the LDH. The existence of RhB between the LDH layers and its expel during the transition into the β phase are proved via fluorescence microscopy, XRD and ssNMR. The activation energies of intercalation and de-intercalation of RhB are computed and show dependence on the cationic ratio of the corresponding LDH. We find that the energies of de- intercalation are systematically higher than those of intercalation proving that the dyes are stabilized due to the probe-brucite sheets interactions.

  5. Solvothermal synthesis of mesoporous slabstone-like TiO{sub 2} and its photodegradation preference in a methyl orange-rhodamine B mixture solution

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Kun; Zhu, Lianjie; Li, Naixuan; Sun, Youguang; Li, Hongbin; Zhu, Xuchen [School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384 (China)

    2012-10-15

    Mesoporous slabstone-like anatase TiO{sub 2} micro-nanometer composite structure has been successfully synthesized by a facile solvothermal method at 180 C using polyethylene glycol (PEG) as a structure-directing agent, followed by calcination at 400 C for 2 h. The crystal structure and morphology of the product were characterized by XRD, SEM, TEM and HRTEM. Its BET specific surface area was obtained from N{sub 2} adsorption-desorption isotherm measurement. Rhodamine B (RB) aqueous solution was used to evaluate the photocatalytic activity of the as-prepared TiO{sub 2} under simulated sunlight irradiation and compared with that of commercial TiO{sub 2} (P25). A RB and methyl orange (MO) coexisting solution was chosen to investigate the photodegradation preference of the slabstone-like TiO{sub 2} on these two dyes. The results show that the photocatalytic activity of the as-prepared TiO{sub 2} is much higher than that of P25, and MO is the preferential degradation species in the MO-RB mixture solution. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  6. Dynamics of Intercalation/De-Intercalation of Rhodamine B during the Polymorphic Transformation of CdAl Layered Double Hydroxide to the Brucite-Like Cadmium Hydroxide

    KAUST Repository

    Saliba, Daniel; Ezzeddine, Alaa; Emwas, Abdul-Hamid M.; Khashab, Niveen M.; Al-Ghoul, Mazen

    2016-01-01

    Cadmium-Aluminum layered double hydroxide (CdAl LDH) is thermodynamically unstable and transforms to Cd(OH)2 and Al(OH)3 in a short period of time. We present a reaction-diffusion framework that enables us to use in situ steady-state fluorescence spectroscopy to study the kinetics of intercalation of a fluorescent probe (Rhodamine B (RhB)) during the formation of the CdAl LDH and its de-intercalation upon the conversion of the LDH phase to the β phase (Cd(OH)2). The method involves the diffusion of sodium hydroxide into a hydrogel gel matrix containing the aluminum and cadmium ions as well as the species we wish to incorporate in the interlayers of the LDH. The existence of RhB between the LDH layers and its expel during the transition into the β phase are proved via fluorescence microscopy, XRD and ssNMR. The activation energies of intercalation and de-intercalation of RhB are computed and show dependence on the cationic ratio of the corresponding LDH. We find that the energies of de- intercalation are systematically higher than those of intercalation proving that the dyes are stabilized due to the probe-brucite sheets interactions.

  7. Turn-On Fluorescent Chemosensor for Hg2+ Based on Multivalent Rhodamine Ligands

    Science.gov (United States)

    Wang, Xuemei; Iqbal, Mudassir; Huskens, Jurriaan; Verboom, Willem

    2012-01-01

    Rhodamine-based fluorescent chemosensors 1 and 2 exhibit selective fluorescence enhancement to Fe3+ and Hg2+ over other metal ions at 580 nm in CH3CN/H2O (3/1, v/v) solution. Bis(rhodamine) chemosensor 1, under optimized conditions (CH3CN/HEPES buffer (0.02 M, pH = 7.0) (95/5, v/v)), shows a high selectivity and sensitivity to Hg2+, with a linear working range of 0–50 μM, a wide pH span of 4–10, and a detection limit of 0.4 μM Hg2+. PMID:23222686

  8. Spectrophotometric procedure using rhodamine B for determination of submicrogram quantities of antimony in rocks

    Science.gov (United States)

    Schnepfe, M.M.

    1973-01-01

    A spectrophotometric procedure using Rhodamine B is given for the determination of antimony in mineralized rocks after its separation as stibine. A study of the Rhodamine B reaction points to the importance of the order of addition of reagents in enhancing sensitivity and increasing the stability of the system. The tolerance of some 26 elements is established for the overall procedure. Although the limit of determination is approximately 0??5 ppm Sb in a 0??2-g sample, the procedure is intended primarily for screening samples containing more than 1 ppm Sb. In pure solutions 0??1 ??g of antimony can be determined with a relative standard deviation of 25%. For >0??2 ??g of antimony a relative standard deviation of 15% or less can be expected. ?? 1973.

  9. Ratiometric Signaling of Hypochlorite by the Oxidative Cleavage of Sulfonhydrazide-Based Rhodamine-Dansyl Dyad.

    Science.gov (United States)

    Lee, Hyo Jin; Cho, Min Jeoung; Chang, Suk-Kyu

    2015-09-08

    A reaction-based probe 1 for hypochlorite signaling was designed by the conjugation of two fluorophores, rhodamine and dansyl moieties, by the reaction of rhodamine B base with dansylhydrazine. Probe 1 exhibited pronounced hypochlorite-selective chromogenic and fluorescent signaling behavior over other oxidants used in practical applications, such as hydrogen peroxide, peracetic acid, and ammonium persulfate, as well as commonly encountered metal ions and anions. Signaling was attributed to the hypochlorite-induced oxidative cleavage of the sulfonhydrazide linkage of the probe. In particular, favorable ratiometric fluorescence signaling was possible by utilizing the emissions of the two fluorophores. A detection limit of 1.13 × 10(-6) M (0.058 ppm) was estimated for the determination of hypochlorite. A paper-based test strip was prepared and was used as a semiquantitative indicator for the presence of hypochlorite in aqueous solutions. The probe was also successfully applied for the determination of hypochlorite in practical tap water samples.

  10. A thiourea-appended rhodamine chemodosimeter for mercury(II) and its bioimaging application

    Science.gov (United States)

    Tantipanjaporn, Ajcharapan; Prabpai, Samran; Suksen, Kanoknetr; Kongsaeree, Palangpon

    2018-03-01

    A rhodamine-thiourea conjugate RTP with an o-phenylenediamine linker was developed as a fluorogenic chemodosimeter for Hg2+ detection. In the presence of Hg2+, a colorless solution of RTP turned pink with a maximum absorption band at 555 nm and with a 62-fold fluorescence enhancement at 578 nm (Φ = 0.34). RTP is highly selective to Hg2+ among other metal ions with a detection limit of 1.6 nM (0.3 ppb). A similar rhodamine analog with a flexible ethylenediamine spacer was less selective and less sensitive than RTP. Hg2+ induced cyclic guanylation to yield a benzimidazole moiety and a subsequent ring-opening of the spirolactam unit resulted in chromogenic and fluorogenic changes. The membrane-permeable RTP probe was successfully demonstrated in monitoring of Hg2+ in cultured HeLa cells.

  11. Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer.

    Science.gov (United States)

    Koide, Yuichiro; Urano, Yasuteru; Hanaoka, Kenjiro; Terai, Takuya; Nagano, Tetsuo

    2011-06-17

    The absorption and emission wavelengths of group 14 pyronines and rhodamines, which contain silicon, germanium, or tin at the 10 position of the xanthene chromophore, showed large bathochromic shifts compared to the original rhodamines, owing to stabilization of the LUMO energy levels by σ*-π* conjugation between group 14 atom-C (methyl) σ* orbitals and a π* orbital of the fluorophore. These group 14 pyronines and rhodamines retain the advantages of the original rhodamines, including high quantum efficiency in aqueous media (Φ(fl) = 0.3-0.45), tolerance to photobleaching, and high water solubility. Group 14 rhodamines have higher values of reduction potential than other NIR light-emitting original rhodamines, and therefore, we speculated their NIR fluorescence could be controlled through the photoinduced electron transfer (PeT) mechanism. Indeed, we found that the fluorescence quantum yield (Φ(fl)) of Si-rhodamine (SiR) and Ge-rhodamine (GeR) could be made nearly equal to zero, and the threshold level for fluorescence on/off switching lies at around 1.3-1.5 V for the SiRs. This is about 0.1 V lower than in the case of TokyoGreens, in which the fluorophore is well established to be effective for PeT-based probes. That is to say, the fluorescence of SiR and GeR can be drastically activated by more than 100-fold through a PeT strategy. To confirm the validity of this strategy for developing NIR fluorescence probes, we employed this approach to design two kinds of novel fluorescence probes emitting in the far-red to NIR region, i.e., a series of pH-sensors for use in acidic environments and a Zn(2+) sensor. We synthesized these probes and confirmed that they work well.

  12. Ion associates of rare earth elements with salicylic acid derivatives and rhodamine B and their analytical application

    Energy Technology Data Exchange (ETDEWEB)

    Tselik, E I; Poluehktov, N S; Mishchenko, V T [AN Ukrainskoj SSR, Odessa. Fiziko-Khimicheskij Inst.

    1979-10-01

    The determination of rare earth elements by extraction photometry (fluorimetric) technique with the use of salicylic acid derivatives and Rhodamine B is reported. The best results in the determination of REE in the form of ionic associates between their acidocomplexes and Rhodamine B are obtained with the use of 3,5-diiodinesalicylic acid. The ratio between components in the compounds formed and the conditions of extraction are determined.

  13. A novel approach to fabricate dye-encapsulated polymeric micro- and nanoparticles by thin film dewetting technique.

    Science.gov (United States)

    Chatterjee, Manosree; Hens, Abhiram; Mahato, Kuldeep; Jaiswal, Namita; Mahato, Nivedita; Nagahanumaiah; Chanda, Nripen

    2017-11-15

    A new method is reported for fabrication of polymeric micro- and nanoparticles from an intermediate patterned surface originated by dewetting of a polymeric thin film. Poly (d, l-lactide-co-glycolide) or PLGA, a biocompatible polymer is used to develop a thin film over a clean glass substrate which dewets spontaneously in the micro-/nano-patterned surface of size range 50nm to 3.5µm. Since another water-soluble polymer, poly vinyl alcohol (PVA) is coated on the same glass substrate before PLGA thin film formation, developed micro-/nano-patterns are easily extracted in water in the form of micro- and nanoparticle mixture of size range 50nm to 3.0µm. This simplified method is also used to effectively encapsulate a dye molecule, rhodamine B inside the PLGA micro-/nanoparticles. The developed dye-encapsulated nanoparticles, PLGA-rhodamine are separated from the mixture and tested for in-vitro delivery application of external molecules inside human lung cancer cells. For the first time, the use of thin film dewetting technique is reported as a potential route for the synthesis of polymeric micro-/nanoparticles and effective encapsulation of external species therein. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. A selectively rhodamine-based colorimetric probe for detecting copper(II) ion.

    Science.gov (United States)

    Zhang, Jiangang; Zhang, Li; Wei, Yanli; Chao, Jianbing; Shuang, Shaomin; Cai, Zongwei; Dong, Chuan

    2014-11-11

    A novel rhodamine derivative 3-bromo-5-methylsalicylaldehyde rhodamine B hydrazone (BMSRH) has been synthesized by reacting rhodamine B hydrazide with 3-bromo-5-methylsalicylaldehyde and developed as a new colorimetric probe for the selective and sensitive detection of Cu2+. Addition of Cu2+ to the solution of BMSRH results in a rapid color change from colorless to red together with an obvious new band appeared at 552 nm in the UV-vis absorption spectra. This change is attributed to the spirocycle form of BMSRH opened via coordination with Cu2+ in a 1:1 stoichiometry and their association constant is determined as 3.2×10(4) L mol(-1). Experimental results indicate that the BMSRH can provide a rapid, selective and sensitive response to Cu2+ with a linear dynamic range 0.667-240 μmol/L. Common interferent ions do not show any interference on the Cu2+ determination. It is anticipated that BMSRH can be a good candidate probe and has potential application for Cu2+ determination. The proposed probe exhibits the following advantages: a quick, simple and facile synthesis. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Foam separation of Rhodamine-G and Evans Blue using a simple separatory bottle system.

    Science.gov (United States)

    Dasarathy, Dhweeja; Ito, Yoichiro

    2017-09-29

    A simple separatory glass bottle was used to improve separation effectiveness and cost efficiency while simultaneously creating a simpler system for separating biological compounds. Additionally, it was important to develop a scalable separation method so this would be applicable to both analytical and preparative separations. Compared to conventional foam separation methods, this method easily forms stable dry foam which ensures high purity of yielded fractions. A negatively charged surfactant, sodium dodecyl sulfate (SDS), was used as the ligand to carry a positively charged Rhodamine-G, leaving a negatively charged Evans Blue in the bottle. The performance of the separatory bottle was tested for separating Rhodamine-G from Evans Blue with sample sizes ranged from 1 to 12mg in preparative separations and 1-20μg in analytical separations under optimum conditions. These conditions including N 2 gas pressure, spinning speed of contents with a magnetic stirrer, concentration of the ligand, volume of the solvent, and concentration of the sample, were all modified and optimized. Based on the calculations at their peak absorbances, Rhodamine-G and Evans Blue were efficiently separated in times ranging from 1h to 3h, depending on sample volume. Optimal conditions were found to be 60psi N 2 pressure and 2mM SDS for the affinity ligand. This novel separation method will allow for rapid separation of biological compounds while simultaneously being scalable and cost effective. Published by Elsevier B.V.

  16. The influence of functional groups on the permeation and distribution of antimycobacterial rhodamine chelators.

    Science.gov (United States)

    Moniz, T; Leite, A; Silva, T; Gameiro, P; Gomes, M S; de Castro, B; Rangel, M

    2017-10-01

    We formerly hypothesized a mechanism whereby the antimycobacterial efficiency of a set of rhodamine labelled iron chelators is improved via the rhodamine fluorophore which enhances the chelators' permeation properties through membranes. To validate our hypothesis in a cellular context and to understand the influence of the structure of the fluorophore on the chelator's uptake and distribution within macrophages we now report comparative confocal microscopy studies performed with a set of rhodamine labelled chelators. We identify the functional groups of the chelator's framework that favor uptake by macrophages and conclude that the antimycobacterial effect is strongly related with the capacity of the chelator to distribute within the host cell and its compartments, a property that is closely related with the chelators' ability to interact with membranes. The quantification of the chelators' interaction with membranes was assessed through measurement of the corresponding partition constants in liposomes. The overall results support that the compounds which are preferentially taken up are the most efficient antimycobacterial chelators and for that reason we infer that the biological activity is modulated by the structural features of the fluorophore. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Toward Protein Structure In Situ: Comparison of Two Bifunctional Rhodamine Adducts of Troponin C

    Science.gov (United States)

    Julien, Olivier; Sun, Yin-Biao; Knowles, Andrea C.; Brandmeier, Birgit D.; Dale, Robert E.; Trentham, David R.; Corrie, John E. T.; Sykes, Brian D.; Irving, Malcolm

    2007-01-01

    As part of a program to develop methods for determining protein structure in situ, sTnC was labeled with a bifunctional rhodamine (BR or BSR), cross-linking residues 56 and 63 of its C-helix. NMR spectroscopy of the N-terminal domain of BSR-labeled sTnC in complex with Ca2+ and the troponin I switch peptide (residues 115–131) showed that BSR labeling does not significantly affect the secondary structure of the protein or its dynamics in solution. BR-labeling was previously shown to have no effect on the solution structure of this complex. Isometric force generation in isolated demembranated fibers from rabbit psoas muscle into which BR- or BSR-labeled sTnC had been exchanged showed reduced Ca2+-sensitivity, and this effect was larger with the BSR label. The orientation of rhodamine dipoles with respect to the fiber axis was determined by polarized fluorescence. The mean orientations of the BR and BSR dipoles were almost identical in relaxed muscle, suggesting that both probes accurately report the orientation of the C-helix to which they are attached. The BSR dipole had smaller orientational dispersion, consistent with less flexible linkers between the rhodamine dipole and cysteine-reactive groups. PMID:17483167

  18. Advanced oxidation of rhodamine B with hydrogen peroxide over ZnCr layered double hydroxide catalysts

    Directory of Open Access Journals (Sweden)

    Nguyen Tien Thao

    2017-09-01

    Full Text Available Zn/Cr layered zinc hydroxide materials with different molar ratios of Cr/Zn have been synthesized through the coprecipitation method at pH of 9.0–9.5. At high Cr/Zn molar ratios of 0.5/1–1/3, the materials possess some layered structure with carbonate anions between the interlayer galleries. The catalysts present uniform particle sizes and quite high surface area. An isomorphous substitution of Zn2+ by Cr3+ in the brucite-like sheets makes the layered Cr-doped zinc hydroxides potential catalysts for efficient oxidation of rhodamine B with H2O2 solution. The experimental results indicated that the intra-lattice Cr3+ ions are more active than Cr2O3 components in the oxidative removal of rhodamine B. The degradation efficiency is dependent on the intra lattice Cr3+ contents and reaction variables. The Cr/Zn LDH gave a high decolorization (99% of rhodamine B at near neutral pH and room temperature.

  19. Development of AVLIS dye laser system

    International Nuclear Information System (INIS)

    Sugiyama, Akira; Nakayama, Tsuyoshi; Kato, Masaaki; Arisawa, Takashi

    1995-01-01

    CVL pumped single mode dye laser was performed. It was found that pressure tuning has some excellent feature in comparison to mechanical tuning in dye laser frequency control. For evaluation of dye laser amplifier, two-dimensional rate equation was proposed. Calculated data by this equation agreed with experimental data in large diameter input dye laser beam condition. (author)

  20. Identification of 5g and 6g terms and revised ionization energies in the Yb II 4f14nl isoelectronic sequence

    International Nuclear Information System (INIS)

    Sugar, J.; Kaufman, V.

    1979-01-01

    The 5f-5g transitions in Lu III through Os VIII and the 5f-6g transitions in Hf IV through W VI were identified and used to redetermine the ionization energies of Yb II, Lu III, W VI, Re VII, and Os VIII. Complete line-lists and energy levels are given for the one-electron spectra Hf IV, W VI and Os VIII

  1. Identification of 5g and 6g terms and revised ionization energies in the Yb II 4f/sup 14/nl isoelectronic sequence

    Energy Technology Data Exchange (ETDEWEB)

    Sugar, J.; Kaufman, V.

    1979-01-01

    The 5f-5g transitions in Lu III through Os VIII and the 5f-6g transitions in Hf IV through W VI were identified and used to redetermine the ionization energies of Yb II, Lu III, W VI, Re VII, and Os VIII. Complete line-lists and energy levels are given for the one-electron spectra Hf IV, W VI and Os VIII.

  2. Treatment of dye house effluents

    International Nuclear Information System (INIS)

    Waheed, S.; Ashraf, C.M.

    1999-01-01

    Environmental considerations play an increasingly important role in processing of textiles. For textile, limits on particular substances have been and are being laid down either by law or as a result of the demands of clothing manufactures. One of the most complex areas in textile processing is textile printing and dyeing. Here, virtually all dye classes are used. In some printing processes such as reactive printing, many of products used end up in the wastewater. A study of the optimisation of wastewater treatment systems and the systematic management of water and the problems of dyeing are reviewed in this article. (author)

  3. Degradation of a xanthene dye by Fe(II)-mediated activation of Oxone process.

    Science.gov (United States)

    Wang, Y R; Chu, W

    2011-02-28

    A powerful oxidation process using sulfate radicals activated by transition metal mediated Oxone process has been evaluated in depth by monitoring the degradation of a xanthene dye Rhodamine B (RhB) in aqueous solution. Ferrous ion was chosen as the transition metal due to its potential catalytic effect and wide availability in dyeing industrial effluent. The effects of parameters including reactant dosing sequence, Fe(II)/Oxone molar ratio and concentration, solution pH, and inorganic salts on the process performance have been investigated. Total RhB removal was obtained within 90 min under an optimal Fe(II)/Oxone molar ratio of 1:1. The RhB degradation was found to be a two-stage kinetics, consisting of a rapid initial decay and followed by a retarded stage. Additionally, experimental results indicated that the presence of certain anions had either a positive or negative effect on the process. The inhibitory effect in the presence of SO(4)(2-) was elucidated by a proposed formula using Nernst equation. Furthermore, dye mineralization in terms of TOC removal indicates that stepwise addition of Fe(II) and Oxone can significantly improve the process performance by about 20%, and the retention time required can be greatly reduced comparing with the conventional one-off dosing method. Copyright © 2010 Elsevier B.V. All rights reserved.

  4. Genomic and Transcriptomic Associations Identify a New Insecticide Resistance Phenotype for the Selective Sweep at the Cyp6g1 Locus of Drosophila melanogaster.

    Science.gov (United States)

    Battlay, Paul; Schmidt, Joshua M; Fournier-Level, Alexandre; Robin, Charles

    2016-08-09

    Scans of the Drosophila melanogaster genome have identified organophosphate resistance loci among those with the most pronounced signature of positive selection. In this study, the molecular basis of resistance to the organophosphate insecticide azinphos-methyl was investigated using the Drosophila Genetic Reference Panel, and genome-wide association. Recently released full transcriptome data were used to extend the utility of the Drosophila Genetic Reference Panel resource beyond traditional genome-wide association studies to allow systems genetics analyses of phenotypes. We found that both genomic and transcriptomic associations independently identified Cyp6g1, a gene involved in resistance to DDT and neonicotinoid insecticides, as the top candidate for azinphos-methyl resistance. This was verified by transgenically overexpressing Cyp6g1 using natural regulatory elements from a resistant allele, resulting in a 6.5-fold increase in resistance. We also identified four novel candidate genes associated with azinphos-methyl resistance, all of which are involved in either regulation of fat storage, or nervous system development. In Cyp6g1, we find a demonstrable resistance locus, a verification that transcriptome data can be used to identify variants associated with insecticide resistance, and an overlap between peaks of a genome-wide association study, and a genome-wide selective sweep analysis. Copyright © 2016 Battlay et al.

  5. Survery on Actual Conditions of Food Dyes

    OpenAIRE

    佐藤, ひろみ

    1981-01-01

    Many food dyes are widely used as food additives in Japan, and many investigations have been pointed the problems of safety of these food dyes used in Japanese food. There are two types of commercial food dyes, one is synthetic dyes and the other is natural dyes.Recently Japanese food is not stained so colourfully, but it is stained faintly in colour near to natural food by using of mixed synthetic dyes. On their hand, many consumers have a tendency to prefer natural food dyes because they ha...

  6. Dye sensitized photoelectrochemical immunosensor for the tumor marker CEA by using a flower-like 3D architecture prepared from graphene oxide and MoS2.

    Science.gov (United States)

    Song, Kaijing; Ding, Chuanmin; Zhang, Bing; Chang, Honghong; Zhao, Zhihuan; Wei, Wenlong; Wang, Junwen

    2018-06-01

    The authors describe a dye-sensitized photoelectrochemical immunoassay for the tumor marker carcinoembryonic antigen (CEA). The method employs the rhodamine dye Rh123 with red color and absorption maximum at 500 nm for spectral sensitization, and a 3D nanocomposite prepared from graphene oxide and MoS 2 acting as the photoelectric conversion layer. The nanocomposite with flower-like 3D architectures was characterized by transmission electron microscopy, scanning electron microscopy, X-ray powder diffraction, and UV-vis diffuse reflectometry. A photoelectrochemical sandwich immunoassay was developed that is based on the use of the nanocomposite and based on the specific binding of antibody and antigen, and by using a secondary antibody labeled with Rh123 and CdS (Ab 2 -Rh123@CdS). Under optimal conditions and at a typical working voltage of 0 V (vs. Hg/HgCl 2 ), the photocurrent increases linearly 10 pg mL -1 to 80 ng mL -1 CEA concentration range, with a 3.2 pg mL -1 detection limit. Graphical abstract Flower-like GO-MoS 2 complex with high efficiency of electron transport was synthesized to construct photoelectrochemical platform. The sandwich-type immunoassay was built on this platform based on specific binding of antigen and antibody. Carcinoembryonic antigen in sample was detected sensitively by using sensitization of rhodamine dye Rh123 as signal amplification strategy.

  7. Optimization of a heterogeneous catalytic hydrodynamic cavitation reactor performance in decolorization of Rhodamine B: application of scrap iron sheets.

    Science.gov (United States)

    Basiri Parsa, Jalal; Ebrahimzadeh Zonouzian, Seyyed Alireza

    2013-11-01

    A low pressure pilot scale hydrodynamic cavitation (HC) reactor with 30 L volume, using fixed scrap iron sheets, as the heterogeneous catalyst, with no external source of H2O2 was devised to investigate the effects of operating parameters of the HC reactor performance. In situ generation of Fenton reagents suggested an induced advanced Fenton process (IAFP) to explain the enhancing effect of the used catalyst in the HC process. The reactor optimization was done based upon the extent of decolorization (ED) of aqueous solution of Rhodamine B (RhB). To have a perfect study on the pertinent parameters of the heterogeneous catalyzed HC reactor, the following cases as, the effects of scrap iron sheets, inlet pressure (2.4-5.8 bar), the distance between orifice plates and catalyst sheets (submerged and inline located orifice plates), back-pressure (2-6 bar), orifice plates type (4 various orifice plates), pH (2-10) and initial RhB concentration (2-14 mg L(-1)) have been investigated. The results showed that the highest cavitational yield can be obtained at pH 3 and initial dye concentration of 10 mg L(-1). Also, an increase in the inlet pressure would lead to an increase in the ED. In addition, it was found that using the deeper holes (thicker orifice plates) would lead to lower ED, and holes with larger diameter would lead to the higher ED in the same cross-sectional area, but in the same holes' diameters, higher cross-sectional area leads to the lower ED. The submerged operation mode showed a greater cavitational effects rather than the inline mode. Also, for the inline mode, the optimum value of 3 bar was obtained for the back-pressure condition in the system. Moreover, according to the analysis of changes in the UV-Vis spectra of RhB, both degradation of RhB chromophore structure and N-deethylation were occurred during the catalyzed HC process. Copyright © 2013 Elsevier B.V. All rights reserved.

  8. Biological characterization of F-18-labeled rhodamine B, a potential positron emission tomography perfusion tracer.

    Science.gov (United States)

    Bartholomä, Mark D; He, Huamei; Pacak, Christina A; Dunning, Patricia; Fahey, Frederic H; McGowan, Francis X; Cowan, Douglas B; Treves, S Ted; Packard, Alan B

    2013-11-01

    Myocardial infarction is the leading cause of death in western countries, and positron emission tomography (PET) plays an increasing role in the diagnosis and treatment planning for this disease. However, the absence of an (18)F-labeled PET myocardial perfusion tracer hampers the widespread use of PET in myocardial perfusion imaging (MPI). We recently reported a potential MPI agent based on (18)F-labeled rhodamine B. The goal of this study was to more completely define the biological properties of (18)F-labeled rhodamine B with respect to uptake and localization in an animal model of myocardial infarction and to evaluate the uptake (18)F-labeled rhodamine B by cardiomyocytes. A total of 12 female Sprague Dawley rats with a permanent ligation of the left anterior descending artery (LAD) were studied with small-animal PET. The animals were injected with 100-150 μCi of (18)F-labeled rhodamine B diethylene glycol ester ([(18)F]RhoBDEGF) and imaged two days before ligation. The animals were imaged again two to ten days post-ligation. After the post-surgery scans, the animals were euthanized and the hearts were sectioned into 1mm slices and myocardial infarct size was determined by phosphorimaging and 2,3,5-triphenyltetrazolium chloride staining (TTC). In addition, the uptake of [(18)F]RhoBDEGF in isolated rat neonatal cardiomyocytes was determined by fluorescence microscopy. Small-animal PET showed intense and uniform uptake of [(18)F]RhoBDEGF throughout the myocardium in healthy rats. After LAD ligation, well defined perfusion defects were observed in the PET images. The defect size was highly correlated with the infarct size as determined ex vivo by phosphorimaging and TTC staining. In vitro, [(18)F]RhoBDEGF was rapidly internalized into rat cardiomyocytes with ~40 % of the initial activity internalized within the 60 min incubation time. Fluorescence microscopy clearly demonstrated localization of [(18)F]RhoBDEGF in the mitochondria of rat cardiomyocytes. Fluorine-18

  9. Biological characterization of F-18-labeled rhodamine B, a potential positron emission tomography perfusion tracer

    International Nuclear Information System (INIS)

    Bartholomä, Mark D.; He, Huamei; Pacak, Christina A.; Dunning, Patricia; Fahey, Frederic H.; McGowan, Francis X.; Cowan, Douglas B.; Treves, S. Ted; Packard, Alan B.

    2013-01-01

    Introduction: Myocardial infarction is the leading cause of death in western countries, and positron emission tomography (PET) plays an increasing role in the diagnosis and treatment planning for this disease. However, the absence of an 18 F-labeled PET myocardial perfusion tracer hampers the widespread use of PET in myocardial perfusion imaging (MPI). We recently reported a potential MPI agent based on 18 F-labeled rhodamine B. The goal of this study was to more completely define the biological properties of 18 F-labeled rhodamine B with respect to uptake and localization in an animal model of myocardial infarction and to evaluate the uptake 18 F-labeled rhodamine B by cardiomyocytes. Methods: A total of 12 female Sprague Dawley rats with a permanent ligation of the left anterior descending artery (LAD) were studied with small-animal PET. The animals were injected with 100–150 μCi of 18 F-labeled rhodamine B diethylene glycol ester ([ 18 F]RhoBDEGF) and imaged two days before ligation. The animals were imaged again two to ten days post-ligation. After the post-surgery scans, the animals were euthanized and the hearts were sectioned into 1 mm slices and myocardial infarct size was determined by phosphorimaging and 2,3,5-triphenyltetrazolium chloride staining (TTC). In addition, the uptake of [ 18 F]RhoBDEGF in isolated rat neonatal cardiomyocytes was determined by fluorescence microscopy. Results: Small-animal PET showed intense and uniform uptake of [ 18 F]RhoBDEGF throughout the myocardium in healthy rats. After LAD ligation, well defined perfusion defects were observed in the PET images. The defect size was highly correlated with the infarct size as determined ex vivo by phosphorimaging and TTC staining. In vitro, [ 18 F]RhoBDEGF was rapidly internalized into rat cardiomyocytes with ∼ 40 % of the initial activity internalized within the 60 min incubation time. Fluorescence microscopy clearly demonstrated localization of [ 18 F]RhoBDEGF in the mitochondria

  10. Infantile hemangioma: pulsed dye laser versus surgical therapy

    Science.gov (United States)

    Remlova, E.; Dostalova, T.; Michalusova, I.; Vranova, J.; Jelinkova, H.; Hubacek, M.

    2014-05-01

    Hemangioma is a mesenchymal benign tumor formed by blood vessels. Anomalies affect up to 10% of children and they are more common in females than in males. The aim of our study was to compare the treatment efficacy, namely the curative effect and adverse events, such as loss of pigment and appearance of scarring, between classical surgery techniques and laser techniques. For that reason a group of 223 patients with hemangioma was retrospectively reviewed. For treatment, a pulsed dye laser (PDL) (Rhodamine G, wavelength 595 nm, pulsewidth between 0.45 and 40 ms, spot diameter 7 mm, energy density 9-11 J cm-2) was used and the results were compared with a control group treated with classical surgical therapy under general anesthesia. The curative effects, mainly number of sessions, appearance of scars, loss of pigment, and relapses were evaluated as a marker of successful treatment. From the results it was evident that the therapeutic effects of both systems are similar. The PDL was successful in all cases. The surgery patients had four relapses. Classical surgery is directly connected with the presence of scars, but the system is safe for larger hemangiomas. It was confirmed that the PDL had the optimal curative effect without scars for small lesions (approximately 10 mm). Surgical treatment under general anesthesia is better for large hemangiomas; the disadvantage is the presence of scars.

  11. Hair Dyes and Cancer Risk

    Science.gov (United States)

    ... http://www.fda.gov/aboutfda/centersoffices/officeoffoods/cfsan/default.htm . Selected References Huncharek M, Kupelnick B. Personal use of hair dyes and the risk of bladder cancer: results of a meta-analysis. ...

  12. Ultrasound for low temperature dyeing of wool with acid dye.

    Science.gov (United States)

    Ferrero, F; Periolatto, M

    2012-05-01

    The possibility of reducing the temperature of conventional wool dyeing with an acid levelling dye using ultrasound was studied in order to reach exhaustion values comparable to those obtained with the standard procedure at 98 °C, obtaining dyed samples of good quality. The aim was to develop a laboratory method that could be transferred at industrial level, reducing both the energy consumption and fiber damage caused by the prolonged exposure to high temperature without the use of polluting auxiliary agents. Dyeings of wool fabrics were carried out in the temperature range between 60 °C and 80 °C using either mechanical or ultrasound agitation of the bath and coupling the two methods to compare the results. For each dyeing, the exhaustion curves of the dye bath were determined and the better results of dyeing kinetics were obtained with ultrasound coupled with mechanical stirring. Hence the corresponding half dyeing times, absorption rate constants according to Cegarra-Puente modified equation and ultrasonic efficiency were calculated in comparison with mechanical stirring alone. In the presence of ultrasound the absorption rate constants increased by at least 50%, at each temperature, confirming the synergic effect of sonication on the dyeing kinetics. Moreover the apparent activation energies were also evaluated and the positive effect of ultrasound was ascribed to the pre-exponential factor of the Arrhenius equation. It was also shown that the effect of ultrasound at 60 °C was just on the dye bath, practically unaffecting the wool fiber surface, as confirmed by the results of SEM analysis. Finally, fastness tests to rubbing and domestic laundering yielded good values for samples dyed in ultrasound assisted process even at the lower temperature. These results suggest the possibility, thanks to the use of ultrasound, to obtain a well equalized dyeing on wool working yet at 60°C, a temperature process strongly lower than 98°C, currently used in industry

  13. Mesoporous cerium oxide nanospheres for the visible-light driven photocatalytic degradation of dyes

    Directory of Open Access Journals (Sweden)

    Subas K. Muduli

    2014-04-01

    Full Text Available A facile, solvothermal synthesis of mesoporous cerium oxide nanospheres is reported for the purpose of the photocatalytic degradation of organic dyes and future applications in sustainable energy research. The earth-abundant, relatively affordable, mixed valence cerium oxide sample, which consists of predominantly Ce7O12, has been characterized by powder X-ray diffraction, X-ray photoelectron and UV–vis spectroscopy, and transmission electron microscopy. Together with N2 sorption experiments, the data confirms that the new cerium oxide material is mesoporous and absorbs visible light. The photocatalytic degradation of rhodamin B is investigated with a series of radical scavengers, suggesting that the mechanism of photocatalytic activity under visible-light irradiation involves predominantly hydroxyl radicals as the active species.

  14. Surfactant-free solvothermal synthesis of Hydroxyapatite nested bundles for the effective photodegradation of cationic dyes

    Science.gov (United States)

    Reeta Mary, I.; Sonia, S.; Navadeepthy, D.; Mangalaraj, D.; Viswanathan, C.; Ponpandian, N.

    2018-05-01

    In this study, hydroxyapatite nested bundles (HNBs) were successfully constructed from nanosticks as nanoscale building blocks via a facile, solvothermal process without using any surfactant. The fabricated HNBs were structurally analyzed using X-ray diffraction and Fourier transform infrared spectroscopy, which confirmed the purity of the HNBs. The surface characteristics were determined by field emission scanning electron microscopy and Brunauer-Emmett-Teller analysis, and the optical characteristics by ultraviolet (UV)-visible spectroscopy. The synthesized HNBs were tested to determine their activity during the degradation of methylene blue, methylene violet, and rhodamine B via photocatalysis under UV irradiation. The degradation efficiency of HNBs and the rate of degradation can be explained based on the properties of the HNBs and cationic dyes.

  15. Gold nanoworms immobilized graphene oxide polymer brush nanohybrid for catalytic degradation studies of organic dyes

    Science.gov (United States)

    Mogha, Navin Kumar; Gosain, Saransh; Masram, Dhanraj T.

    2017-02-01

    In the present work, we report gold nanoparticles (AuNPs) on poly (dimethylaminoethyl methacrylate) (PDMAEMA) brushes immobilized reduced graphene oxide (Au/PDMAEMA/RGO) as catalyst for degradation kinetic studies of Rhodamine B (RB), Methyl Orange (MO) and Eosine Y (EY) dyes, having an excellent catalytic activity, as evident by the apparent rate constant (kapp), which is found to be 21.8, 26.2, and 8.7 (×10-3 s-1), for RB, MO and EY respectively. Au/PDMAEMA/RGO catalyst is easy to use, highly efficient, recyclable, which make it suitable for applications in waste water management. Foremost, synthesis of PDMAEMA brushes on graphene oxide is accomplished by Atom transfer radical polymerization method (ATRP), whereas AuNPs are synthesized by simple chemical reduction method.

  16. High stability of polymer optical fiber with dye doped cladding for illumination systems

    Energy Technology Data Exchange (ETDEWEB)

    Jaramillo-Ochoa, L. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230, México (Mexico); Narro-García, R. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230, México (Mexico); Universidad Autónoma de Chihuahua, Facultad de Ingeniería, Circuito Universitario S/N, 31125 Chihuahua, Chih., México (Mexico); Ocampo, M.A. [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230, México (Mexico); Quintero-Torres, R., E-mail: rquintero@fata.unam.mx [Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro 76230, México (Mexico)

    2017-04-15

    In this work, the photodegradation of a polymer optical fiber with Rhodamine doped cladding as a function of illumination time and excitation intensity is presented. To show the effect of photodegradation on different bulk geometries and environments, the photodegradation from a dye doped preform and a PMMA thick film is also evaluated. The reversible and the irreversible degradation of the florescent material were quantified under an established excitation scheme. To this purpose, a four-level system to model the photodegradation rates and its relation with the population of the states is presented and it is used to justify a possible underlying mechanism. The obtained results suggest an increase of one order of magnitude in the stability (lifetime) of the polymer optical fiber with respect to the preform or the thick film geometry stability.

  17. Gold Nanoparticle-Based Detection of Hg(II) in an Aqueous Solution: Fluorescence Quenching and Surface-Enhanced Raman Scattering Study

    International Nuclear Information System (INIS)

    Ganbold, Erdene Ochir; Park, Jin Ho; Ock, Kwang Su; Joo, Sang Woo

    2011-01-01

    We studied the detection of the Hg(II) concentration in an aqueous solution using rhodamine dyes on citrate-reduced Au nanoparticles (NPs). The quenching effect from Au NPs was found to decrease as the Hg(II) concentration increased under our experimental conditions. As the fluorescence signals intensified, the surface-enhanced Raman scattering (SERS) intensities reduced on the contrary due to less rhodamine dyes on Au NPs as the Hg(II) concentration increased. The rhodamine 6G (Rh6G) and rhodamine 123 (Rh123) dyes were examined via fluorescence and SERS measurements depending on Hg(II) concentrations. Fast and easy fluorescence detection of an Hg (II) concentration as low as a few ppm could be achieved by naked eye using citrate-reduced Au NPs

  18. In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B

    Directory of Open Access Journals (Sweden)

    Antoine Berberi

    2014-01-01

    Full Text Available Objectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization of the interfacial gap may ultimately lead to infection and bone resorption. Using Rhodamine B, a sensitive fluorescent tracer dye, we aim in this study to investigate leakage at implant-abutment connection of three implant systems having the same prosthetic interface. Materials and Methods. Twenty-one implants (seven Astra Tech, seven Euroteknika, and seven Dentium with the same prosthetic interface were connected to their original abutments, according to the manufacturers’ recommendation. After determination of the inner volume of each implant systems, the kinetic quantification of leakage was evaluated for each group using Rhodamine B (10−2 M. For each group, spectrophotometric analysis was performed to detect leakage with a fluorescence spectrophotometer at 1 h (T0 and 48 h (T1 of incubation time at room temperature. Results. Astra Tech had the highest inner volume (6.8 μL, compared to Dentium (4 μL and Euroteknika (2.9 μL. At T0 and T1, respectively, the leakage volume and percentage of each system were as follows: Astra Tech 0.043 μL or 1.48% (SD 0.0022, 0.08 μL or 5.56% (SD 0.0074, Euroteknika 0.09 μL or 6.93% (SD 0.0913, 0.21 μL or 20.55% (SD 0.0035, and Dentium 0.07 μL or 4.6% (SD 0.0029, 0.12 μL or 10.47% (SD 0.0072. Conclusion. The tested internal conical implant-abutment connections appear to be unable to prevent leakage. In average, Astra Tech implants showed the highest inner volume and the least leakage.

  19. Preparation of environment-friendly 3D eggshell membrane-supported anatase TiO2 as a reusable photocatalyst for degradation of organic dyes

    Science.gov (United States)

    Li, Yaling; Zhou, Ji; Fan, Yunde; Ye, Yong; Tang, Bin

    2017-12-01

    We fabricated a low-cost and efficient composite photocatalyst material, combining eggshell membrane (ESM) and titanium dioxide (TiO2) nanoparticles, through self-assembly method. ESM with 3D porous structures provide scaffolds for TiO2 nanoparticles. Polyethyleneimine (PEI) was used to modify ESM by grafting amine groups. The microstructure and property of the fabricated composites were studied by various characterization methods. The composite was used for the photodegradation of Rhodamine B (RhB). The results demonstrate that the composite catalyst possesses good photocatalytic performance for dye degradation under sunlight irradiation simulated by a xenon lamp. Functionalization based on nanomaterials may promote the applications of ESM.

  20. Application of Ni-Oxide@TiO2 Core-Shell Structures to Photocatalytic Mixed Dye Degradation, CO Oxidation, and Supercapacitors

    Directory of Open Access Journals (Sweden)

    Seungwon Lee

    2016-12-01

    Full Text Available Performing diverse application tests on synthesized metal oxides is critical for identifying suitable application areas based on the material performances. In the present study, Ni-oxide@TiO2 core-shell materials were synthesized and applied to photocatalytic mixed dye (methyl orange + rhodamine + methylene blue degradation under ultraviolet (UV and visible lights, CO oxidation, and supercapacitors. Their physicochemical properties were examined by field-emission scanning electron microscopy, X-ray diffraction analysis, Fourier-transform infrared spectroscopy, and UV-visible absorption spectroscopy. It was shown that their performances were highly dependent on the morphology, thermal treatment procedure, and TiO2 overlayer coating.

  1. In search of laterally heterogeneous viscosity models of Glacial Isostatic Adjustment with the ICE-6G_C global ice history model

    Science.gov (United States)

    Li, Tanghua; Wu, Patrick; Steffen, Holger; Wang, Hansheng

    2018-05-01

    Most models of Glacial Isostatic Adjustment (GIA) assume that the Earth is laterally homogeneous. However, seismic and geological observations clearly show that the Earth's mantle is laterally heterogeneous. Previous studies of GIA with lateral heterogeneity mostly focused on its effect or sensitivity on GIA predictions, and it is not clear to what extent can lateral heterogeneity solve the misfits between GIA predictions and observations. Our aim is to search for the best 3D viscosity models that can simultaneously fit the global relative sea-level (RSL) data, the peak uplift rates (u-dot from GNSS) and peak gravity-rate-of-change (g-dot from the GRACE satellite mission) in Laurentia and Fennoscandia. However, the search is dependent on the ice and viscosity model inputs - the latter depends on the background viscosity and the seismic tomography models used. In this paper, the ICE-6G_C ice model, with Bunge & Grand's seismic tomography model and background viscosity models close to VM5 will be assumed. A Coupled Laplace-Finite Element Method is used to compute gravitationally self-consistent sea level change with time dependent coastlines and rotational feedback in addition to changes in deformation, gravity and the state of stress. Several laterally heterogeneous models are found to fit the global sea level data better than laterally homogeneous models. Two of these laterally heterogeneous models also fit the ICE-6G_C peak g-dot and u-dot rates observed in Laurentia simultaneously. However, even with the introduction of lateral heterogeneity, no model that is able to fit the present-day g-dot and uplift rate data in Fennoscandia has been found. Therefore, either the ice history of ICE-6G_C in Fennoscandia and Barent Sea needs some modifications, or the sub-lithospheric property/non-thermal effect underneath northern Europe must be different from that underneath Laurentia.

  2. Efflux of rhodamine from CD56+ cells as a surrogate marker for reversal of P-glycoprotein-mediated drug efflux by PSC 833

    DEFF Research Database (Denmark)

    Robey, R; Bakke, S; Stein, W

    1999-01-01

    minutes. A dose-response relationship was shown between the concentration of PSC 833 in the blood and the inhibition of rhodamine efflux, with an apparent plateau of the inhibition of rhodamine efflux at approximately 1,000 ng/mL. The Ki, defined as the concentration required for half-maximal inhibition...... of Pgp-mediated rhodamine efflux, was determined to be in the range of 29 to 181 ng/mL; although results in two patients were distinctly different, with Ki values of 914 and 916 ng/mL. MRK-16 staining was similar among all patients. We conclude that measurement of rhodamine efflux from CD56(+) cells...

  3. Natural dyes as photosensitizers for dye-sensitized solar cell

    Energy Technology Data Exchange (ETDEWEB)

    Hao, Sancun; Wu, Jihuai; Huang, Yunfang; Lin, Jianming [Institute of Materials Physical Chemistry, Huaqiao University, Quanzhou, Fujian 362021 (China)

    2006-02-15

    The dye-sensitized solar cells (DSC) were assembled by using natural dyes extracted from black rice, capsicum, erythrina variegata flower, rosa xanthina, and kelp as sensitizers. The I{sub SC} from 1.142mA to 0.225mA, the V{sub OC} from 0.551V to 0.412V, the fill factor from 0.52 to 0.63, and P{sub max} from 58{mu}W to 327{mu}W were obtained from the DSC sensitized with natural dye extracts. In the extracts of natural fruit, leaves and flower chosen, the black rice extract performed the best photosensitized effect, which was due to the better interaction between the carbonyl and hydroxyl groups of anthocyanin molecule on black rice extract and the surface of TiO{sub 2} porous film. The blue-shift of absorption wavelength of the black rice extract in ethanol solution on TiO{sub 2} film and the blue-shift phenomenon from absorption spectrum to photoaction spectrum of DSC sensitized with black rice extract are discussed in the paper. Because of the simple preparation technique, widely available and low cheap cost natural dye as an alternative sensitizer for dye-sensitized solar cell is promising. (author)

  4. Synthesis of highly phosphonic acid functionalized benzene-bridged periodic mesoporous organosilicas for use as efficient dye adsorbents

    International Nuclear Information System (INIS)

    Deka, Juti Rani; Liu, Chia-Ling; Wang, Tzu-Hua; Chang, Wei-Chieh; Kao, Hsien-Ming

    2014-01-01

    Highlights: • Synthesis of highly phosphonic acid functionalized benzene-bridged PMOs. • Phosphonic acid loaded PMOs as adsorbent for cationic and anionic dyes. • Due to electrostatic interaction the adsorbent has high dye adsorption capacity. • π–π stacking interaction between benzene and dye enhances adsorption capacity. • Intraparticle diffusion played a dominant role in the adsorption process. - Abstract: Periodic mesoporous organosilicas (PMOs) with benzene bridging groups in the silica wall were functionalized with a tunable content of phosphonic acid groups. These bifunctional materials were synthesized by co-condensation of two different organosilane precursors, that is, 1,4-bis(triethoxysilyl)benzene (BTEB) and sodium 3-(trihydroxysilyl)propyl methyl phosphate (SPMP), under acidic conditions using nonionic surfactant Brij-S10 as template. The materials exhibited well-ordered mesostructures and were characterized by X-ray diffraction, nitrogen sorption, TEM, TGA, FTIR, and solid-state NMR measurements. The materials thus obtained were employed as adsorbents to remove different types of dyes, for example, cationic dyes methylene blue and phenosafranine, anionic orange II, and amphoteric rhodamine B, from aqueous solutions. The materials exhibited a remarkably high adsorption capacity than activated carbon due to their ordered mesostructures, a large number of phosphonic acid groups, and high surface areas. The adsorption was mainly governed by electrostatic interaction, but also involved π–π stacking interaction as well as hydrogen bonding. The adsorption kinetics can be better fitted by the pseudo-second order model. The adsorption process was controlled by the mechanisms of external mass transfer and intraparticle diffusion. The materials retained more than 97% dye removal efficiency after use for five consecutive cycles

  5. Derivatives of Rhodamine 19 as Mild Mitochondria-targeted Cationic Uncouplers*

    Science.gov (United States)

    Antonenko, Yuri N.; Avetisyan, Armine V.; Cherepanov, Dmitry A.; Knorre, Dmitry A.; Korshunova, Galina A.; Markova, Olga V.; Ojovan, Silvia M.; Perevoshchikova, Irina V.; Pustovidko, Antonina V.; Rokitskaya, Tatyana I.; Severina, Inna I.; Simonyan, Ruben A.; Smirnova, Ekaterina A.; Sobko, Alexander A.; Sumbatyan, Natalia V.; Severin, Fedor F.; Skulachev, Vladimir P.

    2011-01-01

    A limited decrease in mitochondrial membrane potential can be beneficial for cells, especially under some pathological conditions, suggesting that mild uncouplers (protonophores) causing such an effect are promising candidates for therapeutic uses. The great majority of protonophores are weak acids capable of permeating across membranes in their neutral and anionic forms. In the present study, protonophorous activity of a series of derivatives of cationic rhodamine 19, including dodecylrhodamine (C12R1) and its conjugate with plastoquinone (SkQR1), was revealed using a variety of assays. Derivatives of rhodamine B, lacking dissociable protons, showed no protonophorous properties. In planar bilayer lipid membranes, separating two compartments differing in pH, diffusion potential of H+ ions was generated in the presence of C12R1 and SkQR1. These compounds induced pH equilibration in liposomes loaded with the pH probe pyranine. C12R1 and SkQR1 partially stimulated respiration of rat liver mitochondria in State 4 and decreased their membrane potential. Also, C12R1 partially stimulated respiration of yeast cells but, unlike the anionic protonophore FCCP, did not suppress their growth. Loss of function of mitochondrial DNA in yeast (grande-petite transformation) is known to cause a major decrease in the mitochondrial membrane potential. We found that petite yeast cells are relatively more sensitive to the anionic uncouplers than to C12R1 compared with grande cells. Together, our data suggest that rhodamine 19-based cationic protonophores are self-limiting; their uncoupling activity is maximal at high membrane potential, but the activity decreases membrane potentials, which causes partial efflux of the uncouplers from mitochondria and, hence, prevents further membrane potential decrease. PMID:21454507

  6. Photoluminescence of urea- and urea/rhodamine B-capped TiO{sub 2} nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalo-Juan, I., E-mail: gonzalo@materials.tu-darmstadt.de; Macé, L.; Tengeler, S.; Mosallem, A.; Nicoloso, N.; Riedel, R.

    2016-07-01

    Urea- and rhodamine B (RhB)-capped TiO{sub 2} nanoparticles (NPs) have been prepared by solvothermal synthesis and characterized by HRTEM, XRD, FTIR, XPS, optical absorption and photoemission. The urea and urea/RhB ligands are capped to the surface of the TiO{sub 2} NPs for the first time through carbamate bonding. The band gap of TiO{sub 2} is slightly reduced from 3.1 eV to 3.0 eV in the urea capped TiO{sub 2} NPs (TU) and 2.9 eV for the NPs capped with urea/RhB (TUR). The generation of new trapping states in TU and TUR at the conduction band edges (surface oxygen vacancies) has been confirmed by the Urbach law providing tail state energies of 180 meV and 270 meV, respectively. These tail states are considered to be responsible for the strong reduction of the photoluminescence at ≈400 nm and the increased emission at ≈600 nm in TU and TUR. The findings suggest that urea- and RhB-capped TiO{sub 2} NPs could have potential applications as photocatalysts, opto-electronic devices, sensors, biological labels and anti-bacterial agents. - Highlights: • Urea- and urea/rhodamine B (RhB)-capped TiO{sub 2} nanoparticles preparation. • Characterization of optical properties of urea- and urea/rhodamine B (RhB)-capped TiO{sub 2} nanoparticles. • The recombination of electrons and holes is significantly reduced in the capped TiO{sub 2} nanoparticles, in comparison with TiO{sub 2}.

  7. Comment on "An Assessment of the ICE-6G_C (VM5a) Glacial Isostatic Adjustment Model" by Purcell et al.

    Science.gov (United States)

    Richard Peltier, W.; Argus, Donald F.; Drummond, Rosemarie

    2018-02-01

    The most recently published model of the glacial isostatic adjustment process in the ICE-NG (VMX) sequence from the University of Toronto, denoted ICE-6G_C (VM5a), was originally developed to degree and order 256 in spherical harmonics and has been shown to provide accurate fits to a voluminous database of GPS observations from North America, Eurasia, and Antarctica, to time dependent gravity data being provided by the GRACE satellites, and to radiocarbon-dated relative sea level histories through the Holocene epoch. The authors of the Purcell et al. (2016, https://doi.org/10.1002/2015JB012742) paper have suggested this model to be flawed. We have produced a further version of our model, denoted ICE-6G_D (VM5a), by employing the same BEDMAP2 bathymetry for the Southern Ocean as employed in their analysis which has somewhat reduced the differences between our results. However, significant physically important differences remain, including the magnitude of present-day vertical crustal motion in the embayments and in the spectrum of Stokes coefficients for present-day geoid height time dependence which continues to "flatten" at high spherical harmonic degree. We explore the reasons for these differences and trace them to the use by Purcell et al. of a loading history for the embayments that differs significantly from that tabulated for both the original and modified versions of our model.

  8. Application of fluorescent dyes for some problems of bioelectromagnetics

    Science.gov (United States)

    Babich, Danylo; Kylsky, Alexandr; Pobiedina, Valentina; Yakunov, Andrey

    2016-04-01

    Fluorescent organic dyes solutions are used for non-contact measurement of the millimeter wave absorption in liquids simulating biological tissue. There is still not any certain idea of the physical mechanism describing this process despite the widespread technology of microwave radiation in the food industry, biotechnology and medicine. For creating adequate physical model one requires an accurate command of knowledge concerning to the relation between millimeter waves and irradiated object. There were three H-bonded liquids selected as the samples with different coefficients of absorption in the millimeter range like water (strong absorption), glycerol (medium absorption) and ethylene glycol (light absorption). The measurements showed that the greatest response to the action of microwaves occurs for glycerol solutions: R6G (building-up luminescence) and RC (fading luminescence). For aqueous solutions the signal is lower due to lower quantum efficiency of luminescence, and for ethylene glycol — due to the low absorption of microwaves. In the area of exposure a local increase of temperature was estimated. For aqueous solutions of both dyes the maximum temperature increase is about 7° C caused with millimeter waves absorption, which coincides with the direct radio physical measurements and confirmed by theoretical calculations. However, for glycerol solution R6G temperature equivalent for building-up luminescence is around 9° C, and for the solution of ethylene glycol it's about 15°. It is assumed the possibility of non-thermal effect of microwaves on the different processes and substances. The application of this non-contact temperature sensing is a simple and novel method to detect temperature change in small biological objects.

  9. A multifunctional magnetic nanocarrier bearing fluorescent dye for targeted drug delivery by enhanced two-photon triggered release

    International Nuclear Information System (INIS)

    Banerjee, Shashwat S; Chen, D-H

    2009-01-01

    We report a novel nanoformulation for targeted drug delivery which utilizes nanophotonics through the fusion of nanotechnology with biomedical application. The approach involves an energy-transferring magnetic nanoscopic co-assembly fabricated of rhodamine B (RDB) fluorescent dye grafted gum arabic modified Fe 3 O 4 magnetic nanoparticle and photosensitive linker by which dexamethasone drug is conjugated to the magnetic nano-assembly. The advantage offered by this nanoformulation is the indirect photo-triggered-on-demand drug release by efficient up-converting energy of the near-IR (NIR) light to higher energy and intraparticle energy transfer from the dye grafted magnetic nanoparticle to the linker for drug release by cleavage. The synthesized nanoparticles were found to be of ultra-small size (13.33 nm) and are monodispersed in an aqueous suspension. Dexamethasone (Dexa) drug conjugated to RDB-GAMNP by photosensitive linker showed appreciable release of Dexa by photo-triggered response on exposure to radiation having a wavelength in the NIR region whereas no detectable release was observed in the dark. Photo-triggered response for the nanoformulation not bearing the rhodamine B dye was drastically less as less Dexa was released on exposure to NIR radiation which suggest that the photo-cleavage of linker and release of Dexa mainly originated from the indirect excitation through the uphill energy conversions based on donor-acceptor model FRET. The promising pathway of nanophotonics for the on-demand release of the drug makes this nanocarrier very promising for applications in nanomedicine.

  10. Use of Rhodamine B as a biomarker for oral plague vaccination of prairie dogs

    Science.gov (United States)

    Fernandez, Julia Rodriguez-Ramos; Rocke, Tonie E.

    2011-01-01

    Oral vaccination against Yersinia pestis could provide a feasible approach for controlling plague in prairie dogs (Cynomys spp.) for conservation and public health purposes. Biomarkers are useful in wildlife vaccination programs to demonstrate exposure to vaccine baits. Rhodamine B (RB) was tested as a potential biomarker for oral plague vaccination because it allows nonlethal sampling of animals through hair, blood, and feces. We found that RB is an appropriate marker for bait uptake studies of C. ludovicianus) when used at concentrations 10 mg RB per kg target animal mass. Whiskers with follicles provided the best sample for RB detection.

  11. Synthesis and Characterization of Rhodamine B-ethylenediamine-hyaluronan Acid as Potential Biological Functional Materials

    Science.gov (United States)

    Li, Y. L.; Wang, W. X.; Wang, Y.; Zhang, W. B.; Gong, H. M.; Liu, M. X.

    2018-05-01

    The purpose of this study is to synthesize and characterize fluorescent polymers, rhodamine B-ethylenediamine-hyaluronan acid (RhB-EA-HA). RhB-EA-HA was successfully synthesized by ester ammonolysis reaction and amidation reaction. Moreover, the structural properties of RhB-EA-HA were characterized by 1H-NMR spectra, UV-vis spectrometry and Fourier transform infrared spectroscopy (FT-IR). RhB-EA-HA can be grafted on the surface of silica nanomaterials, which may be potential biological functional materials for drug delivery system.

  12. Decolorization Treatment of Copper Phthalocyanine Textile Dye Wastewater by Electrochemical Methods

    Directory of Open Access Journals (Sweden)

    K. Dermentzis

    2013-01-01

    Full Text Available Electrochemical decolorization and degradation treatment of aqueous copper phthalocyanine reactive dye solutions was comparatively studied by electrocoagulation, electrooxidation and electro-Fenton processes. In the electrocoagulation process with aluminum electrodes the colored aqueous solutions of initial pH 6.4 containing 50 mg L-1 copper phthalocyanine and 6 g L-1 NaCl were treated at applied current densities of 2.5 and 5 mA cm-2. Fast and 100% decolorization was achieved in 4 and 2 minutes of electroprocessing respectively. The indirect electrooxidation process was conducted in acidic electrolyte solutions containing 50 mg L-1 copper phthalocyanine and 6 g L-1 NaCl with Ti/Pt and graphite plate electrodes at the applied current density of 10 mA cm-2. Even after 90 minutes of electrolysis time the dye remained by 23 and 18.8 % respectively undegradable. By the direct and indirect electrooxidation with the same amount of Na2SO4 electrolyte and added H2O2 respectively and using the same electrodes, the copper phthalocyanine dye was not or was only barely degraded respectively. In the electro-Fenton process with Fe electrodes and added amounts of H2O2 at pH 3 and an applied current density of 5 mA/cm2 complete degradation of copper phthalocyanine occurred in 15 minutes.

  13. A combined Surface Enhanced Raman Spectroscopy (SERS)/UV-vis approach for the investigation of dye content in commercial felt tip pens inks.

    Science.gov (United States)

    Saviello, Daniela; Trabace, Maddalena; Alyami, Abeer; Mirabile, Antonio; Giorgi, Rodorico; Baglioni, Piero; Iacopino, Daniela

    2018-05-01

    The development of protocols for the protection of the large patrimony of works of art created by felt tip pen media since the 1950's requires detailed knowledge of the main dyes constituting commercial ink mixtures. In this work Surface Enhanced Raman Scattering (SERS) and UV-vis spectroscopy were used for the first time for the systematic identification of dye composition in commercial felt tip pens. A large selection of pens comprising six colors of five different brands was analyzed. Intense SERS spectra were obtained for all colors, allowing identification of main dye constituents. Poinceau 4R and Eosin dyes were found to be the main constituents of red and pink colors; Rhodamine and Tartrazine were found in orange and yellow colors; Erioglaucine was found in green and blue colors. UV-vis analysis of the same inks was used to support SERS findings but also to unequivocally assign some uncertain dye identifications, especially for yellow and orange colors. The spectral data of all felt tip pens collected through this work were assembled in a database format. The data obtained through this systematic investigation constitute the basis for the assembly of larger reference databases that ultimately will support the development of conservation protocols for the long term preservation of modern art collections. Copyright © 2018 Elsevier B.V. All rights reserved.

  14. Recycling of agricultural solid waste, coir pith: Removal of anions, heavy metals, organics and dyes from water by adsorption onto ZnCl2 activated coir pith carbon

    International Nuclear Information System (INIS)

    Namasivayam, C.; Sangeetha, D.

    2006-01-01

    The abundant lignocellulosic agricultural waste, coir pith is used to develop ZnCl 2 activated carbon and applied to the removal of toxic anions, heavy metals, organic compounds and dyes from water. Sorption of inorganic anions such as nitrate, thiocyanate, selenite, chromium(VI), vanadium(V), sulfate, molybdate, phosphate and heavy metals such as nickel(II) and mercury(II) has been studied. Removal of organics such as resorcinol, 4-nitrophenol, catechol, bisphenol A, 2-aminophenol, quinol, O-cresol, phenol and 2-chlorophenol has also been investigated. Uptake of acidic dyes such as acid brilliant blue, acid violet, basic dyes such as methylene blue, rhodamine B, direct dyes such as direct red 12B, congo red and reactive dyes such as procion red, procion orange were also examined to assess the possible use of the adsorbent for the treatment of contaminated ground water. Favorable conditions for maximum removal of all adsorbates at the adsorbate concentration of 20 mg/L were used. Results show that ZnCl 2 activated coir pith carbon is effective for the removal of toxic pollutants from water

  15. A turn-on fluorescent rhodamine-acyl hydrazide for selective detection of Cu{sup 2+} ions

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Jung Won; Jeong, Hyuk; Lee, Min Hee [Dept. of Chemistry, Sookmyung Women' s University, Seoul (Korea, Republic of)

    2017-03-15

    We present a rhodamine-based probe that can detect Cu{sup 2+} ions via Cu{sup 2+}-promoted hydrolysis in aqueous solutions. In solution, the probe is non-fluorescent and colorless, but gives a strong fluorescence at 586 nm and shows a color change to pink on the addition of Cu{sup 2+} ions. We demonstrate that the rhodamine-based probe undergoes Cu{sup 2+}-promoted hydrolysis with a moderate reaction time (within 3 min) and provides a fluorescence off–on change, even in the presence of other competitive metal ions. The rhodamine-based probe shows a linear correlation between increasing fluorescence at 586 nm and the Cu{sup 2+} concentration, and can detect Cu{sup 2+} at nanomolar levels (10 nM) in CH{sub 3}CN:H{sub 2}O (v/v, 90:10)

  16. Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Fluorescence Quenching of Rhodamine B.

    Science.gov (United States)

    Fael, Hanan; Sakur, Amir Al-Haj

    2015-11-01

    A novel, simple and specific spectrofluorimetric method was developed and validated for the determination of perindopril erbumine (PDE). The method is based on the fluorescence quenching of Rhodamine B upon adding perindopril erbumine. The quenched fluorescence was monitored at 578 nm after excitation at 500 nm. The optimization of the reaction conditions such as the solvent, reagent concentration, and reaction time were investigated. Under the optimum conditions, the fluorescence quenching was linear over a concentration range of 1.0-6.0 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the developed and reference methods revealed no significant differences between the methods compared in terms of accuracy and precision. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. The mechanism of rhodamine B quenching was also discussed.

  17. A simple rhodamine hydrazide-based turn-on fluorescent probe for HOCl detection.

    Science.gov (United States)

    Zhang, Zhen; Zou, Yuan; Deng, Chengquan; Meng, Liesu

    2016-06-01

    Hypochlorous acid (HOCl) plays a crucial role in daily life and mediates a variety of physiological processes, however, abnormal levels of HOCl have been associated with numerous human diseases. It is therefore of significant interest to establish a simple, selective, rapid and sensitive fluorogenic method for the detection of HOCl in environmental and biological samples. A hydrazide-containing fluorescent probe based on a rhodamine scaffold was facilely developed that could selectively detect HOCl over other biologically relevant reactive oxygen species, reactive nitrogen species and most common metal ions in vitro. Via an irreversible oxidation-hydrolysis mechanism, and upon HOCl-triggered opening of the intramolecular spirocyclic ring during detection, the rhodamine hydrazide-based probe exhibited large fluorescence enhancement in the emission spectra with a fast response, low detection limit and comparatively wide pH detection range in aqueous media. The probe was further successfully applied to monitoring trace HOCl in tap water and imaging both exogenous and endogenous HOCl within living cells. It is anticipated that this simple and useful probe might be an efficient tool with which to facilitate more HOCl-related chemical and biological research. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

  18. Rhodamine-123: a p-glycoprotein marker complex with sodium lauryl sulfate.

    Science.gov (United States)

    Al-Mohizea, Abdullah M; Al-Jenoobi, Fahad Ibrahim; Alam, Mohd Aftab

    2015-03-01

    Aim of this study was to investigate the role of sodium lauryl sulfate (SLS) as P-glycoprotein inhibitor. The everted rat gut sac model was used to study in-vitro mucosal to serosal transport of Rhodamine-123 (Rho-123). Surprisingly, SLS decreases the serosal absorption of Rho-123 at all investigated concentrations. Investigation reveals complex formation between Rhodamine-123 and sodium lauryl sulfate. Interaction profile of SLS & Rho-123 was studied at variable SLS concentrations. The SLS concentration higher than critical micelle concentration (CMC) increases the solubility of Rho-123 but could not help in serosal absorption, on the contrary the absorption of Rho-123 decreased. Rho-123 and SLS form pink color complex at sub-CMC. The SLS concentrations below CMC decrease the solubility of Rho-123. For further studies, Rho-123 & SLS complex was prepared by using solvent evaporation technique and characterized by using differential scanning calorimeter (DSC). Thermal analysis also proved the formation of complex between SLS & Rho-123. The P values were found to be significant (<0.05) except group comprising 0.0001% SLS, and that is because 0.0001% SLS is seems to be very low to affect the solubility or complexation of Rho-123.

  19. Gold nanorods-enhanced rhodamine B-permanganate chemiluminescence and its analytical application.

    Science.gov (United States)

    Hassanzadeh, Javad; Amjadi, Mohammad; Manzoori, Jamshid L; Sorouraddin, Mohammad Hossein

    2013-04-15

    A novel enhanced chemiluminescence system was developed by applying gold nanorods (Au NRs) as catalysts in rhodamine B-permanganate reaction. Au NRs with three different aspect ratios were synthesized by seed mediated growth method and characterized by UV-Vis spectra and transmission electron microscopy. It was demonstrated that Au NRs have much higher catalytic effect than spherical nanoparticles on rhodamine B-permanganate chemiluminescence reaction. Among various sizes of Au NRs, those with average aspect ratio of 3.0 were found to have the most remarkable catalytic activity. As an analytical application of the new chemiluminescence system, albumin as a model protein was quantified based on its interaction with NRs. Albumin binds to Au NRs active surfaces and inhibits their catalytic action and therefore decreases the intensity of chemiluminescence. This diminution effect is linearly related to the concentration of the human and bovine serum albumin over the ranges of 0.45-90 and 0.75-123 nmol L(-1), respectively with the corresponding limits of detection of 0.18 and 0.30 nmol L(-1). The method was successfully applied to the determination of albumin in human and bovine serum samples. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Off–on switchable chemosensor based on rhodamine armed with morpholine moiety

    Energy Technology Data Exchange (ETDEWEB)

    Dhara, Anamika; Guchhait, Nikhil, E-mail: nguchhait@yahoo.com; Kar, Susanta K., E-mail: skkar_cu@yahoo.co.in

    2015-12-15

    We have synthesized a novel morpholine functionalized rhodamine derivative RBM which specifically binds to Fe{sup 3+} in the presence of large excess of other competing metal ions. The chemosensor is highly selective for Fe{sup 3+} over other cations. Meanwhile, the distinct color changes and rapid switch-on fluorescence also provide “naked eyes” detection for Fe{sup 3+} over a broad pH range. The chemosensor also displays 1:1 complex formation with Fe{sup 3+} ion, with a detection limit of 2.1 µM in aqueous CH{sub 3}CN solution, showing that it may offer potential as a chemosensor for the detection of submillimolar Fe{sup 3+} ions in physiological environments. - Highlights: • Design and synthesis of morpholine functionalized rhodamine derivative RBM. • RBM is a highly selective fluorescence sensor for Fe{sup 3+} via reversible CHEF mechanism. • Distinct color changes “naked-eye” and rapid switch-on fluorescence detection for Fe{sup 3+}. • Detection limit was calculated to be 2.1 μM for Fe{sup 3+} ion.

  1. Natural dyes versus lysochrome dyes in cheiloscopy: A comparative evaluation.

    Science.gov (United States)

    Singh, Narendra Nath; Brave, V R; Khanna, Shally

    2010-01-01

    Cheiloscopy is the study of lip prints. Lip prints are genotypically determined and are unique, and stable. At the site of crime, lip prints can be either visible or latent. To develop lip prints for study purpose various chemicals such as lysochrome dyes, fluorescent dyes, etc. are available which are very expensive. Vermilion (Sindoor used by married Indian women) and indigo dye (fabric whitener) are readily available, naturally derived, and cost-effective reagents available in India. To compare the efficacy of sudan black, vermilion, and indigo in developing visible and latent lip prints made on bone china cup, satin fabric, and cotton fabric. Out of 45 Volunteers 15 lip prints were made on bone China cup 15 lip prints on Satin fabric and 15 on Cotton fabric. Sudan black, vermilion and indigo were applied on visible and latent lip prints and graded as good (+,+), fair (+), and poor (-) and statistically evaluated. The vermilion and indigo dye gives comparable results to that of sudan black for developing visible and latent lip prints.

  2. Effect of dye structure and redox mediators on anaerobic azo and anthraquinone dye reduction

    Directory of Open Access Journals (Sweden)

    Mayara Carantino Costa

    2012-01-01

    Full Text Available We investigated the biological decolourisation of dyes with different molecular structures. The kinetic constant values (k1 achieved with azo dye Reactive Red 120 were 7.6 and 10.1 times higher in the presence of RM (redox mediators AQDS and riboflavin, respectively, than the assays lacking RM. The kinetic constant achieved with the azo dye Congo Red was 42 times higher than that obtained with the anthraquinone dye Reactive Blue 4. The effect of RM on dye reduction was more evident for azo dyes resistant to reductive processes, and ineffective for anthraquinone dyes because of the structural stability of the latter.

  3. High orbital angular momentum states in H2 and D2. II. The 6h--5g and 6g--5f transitions

    International Nuclear Information System (INIS)

    Jungen, C.; Dabrowski, I.; Herzberg, G.; Kendall, D.J.W.

    1989-01-01

    A group of lines accompanying the first line of the Pfund series of the H atom has been observed by Fourier transform infrared spectrometry. The lines are due to transitions in molecular hydrogen of a nonpenetrating Rydberg electron possessing a high-orbital angular momentum, which is coupled only loosely to the vibrations and rotations of the H + 2 core. Lines belonging to the 6h--5g and 6g--5f (v=0--3) transitions of H 2 have been identified. The identifications are based on a calculation of the spectrum from first principles by multichannel quantum defect theory. The interaction between the nonpenetrating electron and the core was evaluated in terms of the permanent and induced molecular moments of H + 2 as calculated by Bishop and collaborators. The analogous transitions in D 2 have also been observed and assigned

  4. A rhodamine-labeled citalopram analogue as a high-affinity fluorescent probe for the serotonin transporter

    DEFF Research Database (Denmark)

    Zhang, Peng; Jørgensen, Trine Nygaard; Løland, Claus Juul

    2013-01-01

    A novel fluorescent ligand was synthesized as a high-affinity, high specificity probe for visualizing the serotonin transporter (SERT). The rhodamine fluorophore was extended from an aniline substitution on the 5-position of the dihydroisobenzofuran ring of citalopram (2, 1-(3-(dimethylamino......)propyl)-1-(4-fluorophenyl)-1,3-dihydroisobenzofuran-5-carbonitrile), using an ethylamino linker. The resulting rhodamine-labeled ligand 8 inhibited [3H]5-HT uptake in COS-7 cells (Ki = 225 nM) with similar potency to the tropane-based JHC 1-064 (1), but with higher specificity towards the SERT relative...

  5. Quirks of dye nomenclature. 1. Evans blue.

    Science.gov (United States)

    Cooksey, C J

    2014-02-01

    The history, origin, identity, chemistry and use of Evans blue dye are described along with the first application to staining by Herbert McLean Evans in 1914. In the 1930s, the dye was marketed under the name, Evans blue dye, which was profoundly more acceptable than the ponderous chemical name.

  6. ISOLASI DAN KARAKTERISASI JAMUR PENDEGRADASI ZAT PEWARNA TEKSTIL (Isolation and Characterization of dye-degrading Fungi

    Directory of Open Access Journals (Sweden)

    Erni Martani

    2011-07-01

    . ABSTRACT The aim of this study was to isolate textile dye degrading fungi from many kinds of sample. Isolation was done using surface plating method on Potato Dextrose Agar medium. Degradation ability was measured based on dye decolorization of agar medium. The selection of isolates was based on ability to decolorize some types of dye, rate of decolorization, and tolerance to dye concentration, respectively. Six kinds dye, namely Basic fuchsin, Crystal violet, Direct blue, Methylene blue, Rhodamine B, and Safranine were used in this study. Six species of lignin degrading white rot fungi were used as positive controls. More than 100 fungal strains could be isolated from waste water and solid wastes of textile and pulp & paper industries, peat soils from Central Kalimantan and Riau, and forest soil. Examination on dye decolorization resulted in 6 selected isolates (coded as JKNT-1, JKSC-1, KRMS 5, TPA-4, TPA-10, and JYGC-1; and 2 species of lignin degrading white rot fungi, namely Phanerochaete chrysosporium and Pleurotus ostreatus. Decolorization of dye was depended on the fungal species and type of dye, i.e. one species decolorized some dyes but not the others. Methylene Blue was decolorized more readily than other dyes. In general, dye decolorization activity of fungal isolates was lower than the lignin degrading fungi. Microscopic examination indicated that the isolates of JKNT 1 and KRMS-5 were come from the genus Penicillium, the genus of JKSC-1 was Stachybotrys, the TPA-4 and JYGC-1 were Cladosporium, and TPA-10 isolate was included in genus of Aspergillus.

  7. Studies on Dyeing Performance of Novel Acid Azo Dyes and Mordent Acid Azo Dyes Based on 2,4-Dihydroxybenzophenone

    Directory of Open Access Journals (Sweden)

    Bharat C. Dixit

    2009-01-01

    Full Text Available Novel acid azo and mordent acid azo dyes have been prepared by the coupling of diazo solution of different aminonaphthol sulphonic acids and aromatic amino acids with 2,4-dihydroxybenzophenone. The resultant dyes were characterized by elemental analysis as well as IR and 1H NMR spectral studies. The UV-visible spectral data have also been discussed in terms of structure property relationship. The dyeing assessments of all the dyes were evaluated on wool and silk textile fibers. The dyeing of chrome pretreated wool and silk have also been monitored. The result shows that better hue was obtained on mordented fiber. Results of bactericidal studies of chrome pretreated fibers revealed that the toxicity of mordented dyes against bacteria is fairly good. Dyeing on wool and silk fibers resulted in yellowish pink to reddish brown colourations having excellent light fastness and washing fastness.

  8. Real-time tunability of chip-based light source enabled by microfluidic mixing

    DEFF Research Database (Denmark)

    Olsen, Brian Bilenberg; Rasmussen, Torben; Balslev, Søren

    2006-01-01

    We demonstrate real-time tunability of a chip-based liquid light source enabled by microfluidic mixing. The mixer and light source are fabricated in SU-8 which is suitable for integration in SU-8-based laboratory-on-a-chip microsystems. The tunability of the light source is achieved by changing...... the concentration of rhodamine 6G dye inside two integrated vertical resonators, since both the refractive index and the gain profile are influenced by the dye concentration. The effect on the refractive index and the gain profile of rhodamine 6G in ethanol is investigated and the continuous tuning of the laser...

  9. [Selected enhancement of different order stokes lines of SRS by using fluorescence of mixed dye solution].

    Science.gov (United States)

    Zuo, Hao-yi; Gao, Jie; Yang, Jing-guo

    2007-03-01

    A new method to enhance the intensity of the different orders of Stokes lines of SRS by using mixed dye fluorescence is reported. The Stokes lines from the second-order to the fifth-order of CCl4 were enhanced by the fluorescence of mixed R6G and RB solutions in different proportions of 20:2, 20:13 and 20:40 (R6g:Rb), respectively. It is considered that the Stokes lines from the second-order to the fifth-order are near the fluorescence peaks of the three mixed solutions, and far from the absorption peaks of R6g and Rb, so the enhancement effect dominates the absorption effect; as a result, these stokes lines are enhanced. On the contrary, the first-order stokes line is near the absorption peak of RB and far from the fluorescence peaks of the mixed solutions, which leads to the weakening of this stokes line. It is also reported that the first-order, the second-order and the third-order Stokes lines of benzene were enhanced by the fluorescence of mixed solutions of R6g and DCM with of different proportions. The potential application of this method is forecasted.

  10. Preparation of magnetic Ni@graphene nanocomposites and efficient removal organic dye under assistance of ultrasound

    International Nuclear Information System (INIS)

    Zhao, Chuang; Guo, Jianhui; Yang, Qing; Tong, Lei; Zhang, Jingwei; Zhang, Jiwei; Gong, Chunhong; Zhou, Jingfang; Zhang, Zhijun

    2015-01-01

    Graphical abstract: Reduced graphene oxide/Ni microspheres, being prepared under ultrasound conditions, exhibit a better removal efficiency to decolorize RhB with ultrasonic-assisted decolorization process. - Highlights: • One-step synthesis of Ni@graphene microspheres under ultrasound conditions. • During the ultrasonic process, graphene oxide was reduced and Ni nanoparticles were formed and anchored on graphene sheets. • The products exhibit excellent performance for fast and efficient removal of dye contaminants. • The nanocomposites can be easily separated from solution by a magnet. - Abstract: In this article, we report a facile one-step synthesis of Ni@graphene nanocomposite microspheres (NGs) in hydrazine hydrate solution under ultrasound conditions. During the ultrasonic process, graphene oxide (GO) was reduced effectively under mild conditions and Ni nanoparticles were simultaneously formed and anchored on graphene sheets, which act as spacers to keep the neighboring sheets separated. The target products exhibit excellent performance for fast and efficient removal of dye contaminants, rhodamine B (RhB) in aqueous solution, under assistance of ultrasound. Finally, the nanocomposites can be easily separated from solution by a magnet. Furthermore, higher content of graphene can be produced under sonication, which facilitates faster and more efficient removal of organic contaminates in the solution. The nanocomposites were also characterized by scanning electron microscopy, Raman spectroscopy, Fourier transformed infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction and thermogravimetric analysis.

  11. Preparation of magnetic Ni@graphene nanocomposites and efficient removal organic dye under assistance of ultrasound

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Chuang; Guo, Jianhui; Yang, Qing; Tong, Lei [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China); Zhang, Jingwei, E-mail: jwzhang@henu.edu.cn [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China); Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095 (Australia); Zhang, Jiwei [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China); Gong, Chunhong [College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004 (China); Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095 (Australia); Zhou, Jingfang, E-mail: jingfang.zhou@unisa.edu.au [Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095 (Australia); Zhang, Zhijun [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China)

    2015-12-01

    Graphical abstract: Reduced graphene oxide/Ni microspheres, being prepared under ultrasound conditions, exhibit a better removal efficiency to decolorize RhB with ultrasonic-assisted decolorization process. - Highlights: • One-step synthesis of Ni@graphene microspheres under ultrasound conditions. • During the ultrasonic process, graphene oxide was reduced and Ni nanoparticles were formed and anchored on graphene sheets. • The products exhibit excellent performance for fast and efficient removal of dye contaminants. • The nanocomposites can be easily separated from solution by a magnet. - Abstract: In this article, we report a facile one-step synthesis of Ni@graphene nanocomposite microspheres (NGs) in hydrazine hydrate solution under ultrasound conditions. During the ultrasonic process, graphene oxide (GO) was reduced effectively under mild conditions and Ni nanoparticles were simultaneously formed and anchored on graphene sheets, which act as spacers to keep the neighboring sheets separated. The target products exhibit excellent performance for fast and efficient removal of dye contaminants, rhodamine B (RhB) in aqueous solution, under assistance of ultrasound. Finally, the nanocomposites can be easily separated from solution by a magnet. Furthermore, higher content of graphene can be produced under sonication, which facilitates faster and more efficient removal of organic contaminates in the solution. The nanocomposites were also characterized by scanning electron microscopy, Raman spectroscopy, Fourier transformed infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction and thermogravimetric analysis.

  12. Removal of Organic Dyes by Nanostructure ZnO-Bamboo Charcoal Composites with Photocatalysis Function

    Directory of Open Access Journals (Sweden)

    Xinliang Yu

    2015-01-01

    Full Text Available Composites of nanostructure zinc oxide (nano-ZnO and bamboo charcoal (BC were successfully prepared via impregnation-precipitation method. The products were characterized by XRD, SEM, and EDS. Rhodamine B (RhB and acid fuchsin (AF were selected as the organic dyes of photocatalysis degradation under the irradiation of ultraviolet light (UV. The influence of particle size of BC, irradiation time, pH value of the solution, and additive amount of H2O2 on removal of the dyes has been studied. The results show that smaller particle size of BC in the composites has a better removal effect. The composites possess the highest removal capacity for RhB and AF under the conditions of pH = 2 and pH = 5.4, respectively. The optimum additive amount of H2O2 for 5 mL RhB and AF was 0.050 mL and 0.1 mL, with a removal rate of 93% and 99%, respectively.

  13. Photoelectrochemical studies of dye-sensitized solar cells using organic dyes

    Energy Technology Data Exchange (ETDEWEB)

    Marinado, Tannia

    2009-10-15

    The dye-sensitized solar cell (DSC) is a promising efficient low-cost molecular photovoltaic device. One of the key components in DSCs is the dye, as it is responsible for the capture of sunlight. State-of-the-art DSC devices, based on ruthenium dyes, show record efficiencies of 10-12 %. During the last decade, metal-free organic dyes have been extensively explored as sensitizers for DSC application. The use of organic dyes is particularly attractive as it enables easy structural modifications, due to fairly short synthetic routes and reduced material cost. Novel dye should in addition to the light-harvesting properties also be compatible with the DSC components. In this thesis, a series of new organic dyes are investigated, both when integrated in the DSC device and as individual components. The evaluation methods consisted of different electrochemical and photoelectrochemical techniques. Whereas the light-harvesting properties of the dyes were fairly easily improved, the behavior of the dye integrated in the DSC showed less predictable photovoltaic results. The dye series studied in Papers II and IV revealed that their dye energetics limited vital electron-transfer processes, the dye regeneration (Paper II) and injection quantum yield (Paper IV). Further, in Papers III-VI, it was observed that different dye structures seemed to alter the interfacial electron recombination with the electrolyte. In addition to the dye structure sterics, some organic dyes appear to enhance the interfacial recombination, possibly due to specific dye-redox acceptor interaction (Paper V). The impact of dye sterical modifications versus the use of coadsorbent was explored in Paper VI. The dye layer properties in the presence and absence of various coadsorbents were further investigated in Paper VII. The core of this thesis is the identification of the processes and properties limiting the performance of the DSC device, aiming at an overall understanding of the compatibility between the

  14. Radiative characteristics of CVL pumped dye laser

    Energy Technology Data Exchange (ETDEWEB)

    Kubo, Uichi; Ishiguro, Takahide

    1987-09-01

    This paper describes the radiative characteristics of CVL pumped dye laser. It is compared YAG-SH (530 nm) with CVL (511, 578 nm) and CVL (511 nm) for pumping source. Influence of solvent in dye laser power was examined. The present experimental results show that efficiency of CVL (511 nm) pumped dye laser was most high. When the dye solution is at a standstill, the efficiency of dye laser with water and Sodium Lauryl Sulfate (S.L.S., 2 %wt.) was most high among the four kinds of solvent. In the condition of dye solution flow, the water and S.L.S. or ethylene glycol was useful solvent for dye laser.

  15. Radiative characteristics of CVL pumped dye laser

    International Nuclear Information System (INIS)

    Kubo, Uichi; Ishiguro, Takahide.

    1987-01-01

    This paper describes the radiative characteristics of CVL pumped dye laser. It is compared YAG-SH (530 nm) with CVL (511, 578 nm) and CVL (511 nm) for pumping source. Influence of solvent in dye laser power was examined. The present experimental results show that efficiency of CVL (511 nm) pumped dye laser was most high. When the dye solution is at a standstill, the efficiency of dye laser with water and Sodium Lauryl Sulfate (S.L.S., 2 %wt.) was most high among the four kinds of solvent. In the condition of dye solution flow, the water and S.L.S. or Ethylene Glycol was useful solvent for dye laser. (author)

  16. BODIPYs for Dye-Sensitized Solar Cells.

    Science.gov (United States)

    Klfout, Hafsah; Stewart, Adam; Elkhalifa, Mahmoud; He, Hongshan

    2017-11-22

    BODIPY, abbreviation of boron-dipyrromethene, is one class of robust organic molecules that has been used widely in bioimaging, sensing, and logic gate design. Recently, BODIPY dyes have been explored for dye-sensitized solar cells (DSCs). Studies demonstrate their potential as light absorbers for the conversion of solar energy to electricity. However, their photovoltaic performance is inferior to many other dyes, including porphyrin dyes. In this review, several synthetic strategies of BODIPY dyes for DSCs and their further functionalization are described. The photophysical properties of dye molecules and their photovoltaic performances in DSCs are summarized. We aim to provide readers a clear picture of the field and expect to shed light on the next generation of BODIPY dyes for their applications in solar energy conversion.

  17. Influence of styryl dyes on blood erythrocytes

    Science.gov (United States)

    Nizomov, Negmat; Barakaeva, Mubaro; Kurtaliev, Eldar N.; Rahimov, Sherzod I.; Khakimova, Dilorom P.; Khodjayev, Gayrat; Yashchuk, Valeriy N.

    2008-08-01

    It was studied the influence of F, Sbt, Sil, Sbo monomer and homodimer Dst-5, Dst-10, Dbt-5, Dbt-10, Dil-10, Dbo-10 styryl dyes on blood erythrocytes of white rats. It was shown that the homodimer styryl dyes Dst-5, Dbt-5 and Dbo-10 decrease the erythrocytes quantity by 1.5-2 times more as compared with monomer dyes Sbt and Sbo. The main cause of dyes different action is the different oxidation degree of intracellular hemoglobin evoked by these dyes. It was established that the observed effects was connected with different penetration of these dyes through membrane of erythrocytes and with interaction of these dyes with albumin localized in membranes of cells.

  18. Polyurethane foam loaded with SDS for the adsorption of cationic dyes from aqueous medium: Multivariate optimization of the loading process.

    Science.gov (United States)

    Robaina, Nicolle F; Soriano, Silvio; Cassella, Ricardo J

    2009-08-15

    This paper reports the development of a new procedure for the adsorption of four cationic dyes (Rhodamine B, Methylene Blue, Crystal Violet and Malachite Green) from aqueous medium employing polyurethane foam (PUF) loaded with sodium dodecylsulfate (SDS) as solid phase. PUF loading process was based on the stirring of 200mg PUF cylinders with acidic solutions containing SDS. The conditions for loading were optimized by response surface methodology (RSM) using a Doehlert design with three variables that were SDS and HCl concentrations and stirring time. Results obtained in the optimization process showed that the stirring time is not a relevant parameter in the PUF loading, evidencing that the transport of SDS from solution to PUF surface is fast. On the other hand, both SDS and HCl concentrations were important parameters causing significant variation in the efficiency of the resulting solid phase for the removal of dyes from solution. At optimized conditions, SDS and HCl concentrations were 4.0 x 10(-4) and 0.90 mol L(-1), respectively. The influence of stirring time was evaluated by univariate methodology. A 20 min stirring time was established in order to make the PUF loading process fast and robust without losing efficiency. The procedure was tested for the removal of the four cationic dyes from aqueous solutions and removal efficiencies always better than 90% were achieved for the two concentrations tested (2.0 x 10(-5) and 1.0 x 10(-4)mol L(-1)).

  19. Study and realisation of a femtosecond dye laser operating at different wavelengths. Ultrashort pulses compression and amplification

    International Nuclear Information System (INIS)

    Georges, Patrick

    1989-01-01

    We present the study and the realization of a passively mode-locked dye laser producing pulses shorter than 100 femto-seconds (10 -13 s). In a ring cavity with an amplifier medium (Rhodamine 60) and a saturable absorber (DODCI), a sequence of four prisms controls the group velocity dispersion and allows the generation of very short pulses. Then we have studied the production of femtosecond pulses at other wavelengths directly from the femtosecond dye laser. For the first rime, 60 fs pulses at 685 nm and pulses shorter than 50 fs between 775 nm and 800 nm have been produced by passive mode locking. These near infrared pulses have been used to study the absorption saturation kinetics in semiconductors multiple quantum wells GaAs/GaAlAs. We have observed a singular behavior of the laser operating at 685 nm and analyzed the produced pulses in terms of optical solitons. To perform time resolved spectroscopy with shortest pulses, we have studied a pulse compressor and a multipass amplifier to increase the pulses energy. Pulses of 20 fs and 10 micro-joules (peak power: 0.5 GW) have been obtained at low repetition rate (10 Hz) and pulses of 16 fs and 0.6 micro-joules pulses have been generated at high repetition rate (11 kHz) using a copper vapor laser. These pulses have been used to study the absorption saturation kinetics of an organic dye (the Malachite Green). (author) [fr

  20. Light-assisted decomposition of dyes over iron-bearing soil clays in the presence of H2O2

    International Nuclear Information System (INIS)

    Wang Zhaohui; Ma Wanhong; Chen Chuncheng; Zhao Jincai

    2009-01-01

    Four types of soil clays from different sites in China have been chosen to simulate chemical remediation of soils contaminated with dyes by light-assisted Fenton-like method. X-Ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and electron spin resonance (ESR) measurements indicated that these soil clays contain iron oxides such as magnetite and hematite, where nondistorted iron active sites (ESR spectra, g = 2.3) predominate. Upon visible or UV irradiation, the soil clays were very effective for the degradation of nonbiodegradable cationic dyes such as Rhodamine B (RhB) by activating H 2 O 2 at neutral pH. The photodegradation rates of RhB were closely related to total Fe content in clays and H 2 O 2 dosage, indicating the mineral-catalyzed Fenton-like reactions operated. Soil organic matters (SOM) would remarkably inhibit the photodecomposition of RhB dye. The reaction products were some low-molecular-weight dicarboxylic acids and their derivatives, all of which are easily biodegradable. A possible mechanism was proposed based on the results obtained by spin-trapping ESR technique.

  1. Visible light assisted photodegradation of halocarbons on the dye modified TiO{sub 2} surface using visible light

    Energy Technology Data Exchange (ETDEWEB)

    Chatterjee, Debabrata; Dasgupta, Shimanti [Chemistry Section, Central Mechanical Engineering Research Institute, Durgapur 713209 (India); N Rao, Nageswar [Wastewater Technology Division, National Environmental Engineering Research Institute, Nagpur 400020 (India)

    2006-05-05

    Visible light assisted photodegradation of various halocarbons, viz. chloro-phenols, trichloroethylene, 1,2-dichloroethane and 1,4-dichlorobenzene, has been achieved on the surface of dye-modified TiO{sub 2} semiconductor. Thionine, eosinY, rhodamineB, methyleneblue, nileblueA and safranineO were used for the modification of TiO{sub 2} semiconductor photocatalyst. After 5h of irradiation with a 150W Xenon lamp, over 55-72% degradation of pollutants has been achieved. A working mechanism involving excitation of surface adsorbed dye, followed by charge injection into the TiO{sub 2} conduction band and formation of reactive {sup *}O{sub 2}{sup -}/{sup *}HO{sub 2} radicals, is proposed for the degradation of the enlisted organics to carbon dioxide. Efficacy of the photocatalytic system towards halocarbon degradation has been discussed in regard to excited state redox properties of the dyes selected for this study. Formation of {sup *}O{sub 2}{sup -} radical has been substantiated by using a chemiluminescent probe luminol. (author)

  2. A review on applicability of naturally available adsorbents for the removal of hazardous dyes from aqueous waste.

    Science.gov (United States)

    Sharma, Pankaj; Kaur, Harleen; Sharma, Monika; Sahore, Vishal

    2011-12-01

    The effluent water of many industries, such as textiles, leather, paper, printing, cosmetics, etc., contains large amount of hazardous dyes. There is huge number of treatment processes as well as adsorbent which are available for the processing of this effluent water-containing dye content. The applicability of naturally available low cast and eco-friendly adsorbents, for the removal of hazardous dyes from aqueous waste by adsorption treatment, has been reviewed. In this review paper, we have provided a compiled list of low-cost, easily available, safe to handle, and easy-to-dispose-off adsorbents. These adsorbents have been classified into five different categories on the basis of their state of availability: (1) waste materials from agriculture and industry, (2) fruit waste, (3) plant waste, (4) natural inorganic materials, and (5) bioadsorbents. Some of the treated adsorbents have shown good adsorption capacities for methylene blue, congo red, crystal violet, rhodamine B, basic red, etc., but this adsorption process is highly pH dependent, and the pH of the medium plays an important role in the treatment process. Thus, in this review paper, we have made some efforts to discuss the role of pH in the treatment of wastewater.

  3. Novel synthesis and initial preclinical evaluation of (18)F-[FDG] labeled rhodamine: a potential PET myocardial perfusion imaging agent.

    Science.gov (United States)

    AlJammaz, Ibrahim; Al-Otaibi, Basim; AlHindas, Hussein; Okarvi, Subhani M

    2015-10-01

    Myocardial perfusion imaging is one of the most commonly performed investigations in nuclear medicine studies. Due to the clinical importance of [(18)F]-fluoro-2-deoxy-D-glucose ([(18)F]-FDG) and its availability in almost every PET center, a new radiofluorinated [(18)F]-FDG-rhodamine conjugate was synthesized using [(18)F]-FDG as a prosthetic group. In a convenient and simple one-step radiosynthesis, [(18)F]-FDG-rhodamine conjugate was prepared in quantitative radiochemical yields, with total synthesis time of nearly 20 min and radiochemical purity of greater than 98%, without the need for HPLC purification, which make these approaches amenable for automation. Biodistribution studies in normal rats at 60 min post-injection demonstrated a high uptake in the heart (>11% ID/g) and favorable pharmacokinetics. Additionally, [(18)F]-FDG-rhodamine showed an extraction value of 27.63%±5.12% in rat hearts. These results demonstrate that [(18)F]-FDG-rhodamine conjugate may be useful as an imaging agent for the positron emission tomography evaluation of myocardial perfusion. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. A first principles study of fluorescence quenching in rhodamine B dimers : how can quenching occur in dimeric species?

    NARCIS (Netherlands)

    Setiawan, Dani; Kazaryan, Andranik; Martoprawiro, Muhamad Abdulkadir; Filatov, Michael

    2010-01-01

    Rhodamine B (RhB) is widely used in chemistry and biology due to its high fluorescence quantum yield. In high concentrations, the quantum yield of fluorescence decreases considerably which is attributed to the formation of RhB dimers. In the present work, a possible mechanism of fluorescence

  5. Chemiluminescence behavior based on oxidation reaction of rhodamine B with cerium(IV) in sulfuric acid medium

    Energy Technology Data Exchange (ETDEWEB)

    Ma Yongjun; Jin Xiaoyong; Zhou Min; Zhang Ziyu; Teng Xiulan; Chen Hui

    2003-08-18

    The chemiluminescence (CL) of the rhodamine B (RhB)-cerium(IV) system was investigated by flow-injection. Rhodamine B was suggested to be a suitable chemiluminescent reagent in acidic conditions. When the concentration of rhodamine B was 100 mg l{sup -1} and cerium sulfate was 1.6 mmol l{sup -1} in sulfuric acid, the chemiluminescent intensity was found to be highest by using 0.3 mol l{sup -1} sulfuric acid as a carrier solution. The particular chemiluminescent system could tolerate such distinct acidic environments that it was utilized for detecting many compounds that are stable in acidic solutions. Furthermore, by virtue of IR, UV-Vis and luminescence spectroscopic measurements, the chemiluminescent behavior of rhodamine B was studied and a possible mechanism for this chemiluminescent reaction was proposed. The emitter was affirmed to be a radical species due to one of the oxidation products of RhB; the chemiluminescent emissive wavelength was about 425 nm.

  6. Octane-Assisted Reverse Micellar Dyeing of Cotton with Reactive Dyes

    Directory of Open Access Journals (Sweden)

    Alan Yiu-lun Tang

    2017-12-01

    Full Text Available In this study, we investigated the computer colour matching (CCM of cotton fabrics dyed with reactive dye using the octane-assisted reverse micellar approach. The aim of this study is to evaluate the colour quality and compare the accuracy between CCM forecasting and simulated dyeing produced by conventional water-based dyeing and octane-assisted reverse micellar dyeing. First, the calibration of dyeing databases for both dyeing methods was established. Standard samples were dyed with known dye concentrations. Computer colour matching was conducted by using the colour difference formula of International Commission on Illumination (CIE L*a*b*. Experimental results revealed that the predicted concentrations were nearly the same as the expected known concentrations for both dyeing methods. This indicates that octane-assisted reverse micellar dyeing system can achieve colour matching as good as the conventional water-based dyeing system. In addition, when comparing the colour produced by the conventional water-based dyeing system and the octane-assisted reverse micellar dyeing system, the colour difference (ΔE is ≤1, which indicates that the reverse micellar dyeing system could be applied for industrial dyeing with CCM.

  7. Artificial evolution of coumarin dyes for dye sensitized solar cells.

    Science.gov (United States)

    Venkatraman, Vishwesh; Abburu, Sailesh; Alsberg, Bjørn Kåre

    2015-11-07

    The design and discovery of novel molecular structures with optimal properties has been an ongoing effort for materials scientists. This field has in general been dominated by experiment driven trial-and-error approaches that are often expensive and time-consuming. Here, we investigate if a de novo computational design methodology can be applied to the design of coumarin-based dye sensitizers with improved properties for use in Grätzel solar cells. To address the issue of synthetic accessibility of the designed compounds, a fragment-based assembly is employed, wherein the combination of chemical motifs (derived from the existing databases of structures) is carried out with respect to user-adaptable set of rules. Rather than using computationally intensive density functional theory (DFT)/ab initio methods to screen candidate dyes, we employ quantitative structure-property relationship (QSPR) models (calibrated from empirical data) for rapid estimation of the property of interest, which in this case is the product of short circuit current (Jsc) and open circuit voltage (Voc). Since QSPR models have limited validity, pre-determined applicability domain criteria are used to prevent unacceptable extrapolation. DFT analysis of the top-ranked structures provides supporting evidence of their potential for dye sensitized solar cell applications.

  8. COMPARATIVE STUDY OF TWO DYEING METHODS USING REACTIVE DYE

    Directory of Open Access Journals (Sweden)

    HINOJOSA Belén

    2016-05-01

    Full Text Available Environment preservation is a common worry not only for people but for companies as well. Industry is more and more concern about the necessity of developing new and more respectful processes. Dye is one of the most important processes in the textile industry but it is also considered as no too safe regarding environment issues. This process uses large amounts of water and generates big volumes of wastewater. Following this issue, new regulations and laws emerge to control the waste generated. This leads to the companies and increased costs in terms of wastewater treatments and high water consumption. In this research we compare two systems on garment finishing application, the conventional bath process and the new Ecofinish system that is able to save water and product. To compare these processes, we carried out a reactive dyeing using both systems in order to determine the quality differences in the final product. For this purpose, the samples have been tested to washing and rubbing fastness, according to UNE EN ISO 105 C10 and UNE- EN ISO 105 X12 standards, respectively. This study confirms that this system achieves water savings and reduces the wastewater produced, getting a good dyeing. This process can be considered as an alternative to the conventional one.

  9. Synthesis of a Cu2+-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging

    Directory of Open Access Journals (Sweden)

    Chunwei Yu

    2014-11-01

    Full Text Available A new fluorescent probe P based on rhodamine for Cu2+ was synthesized and characterized. The new probe P showed high selectivity to Cu2+ over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10−6–1.0 × 10−5 M with a detection limit of 3.3 × 10−7 M Cu2+ in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0. Furthermore, it has been used for imaging of Cu2+ in living cells with satisfying results.

  10. Highly Selective Deethylation of Rhodamine B on Prepared in Supercritical Fluids

    Directory of Open Access Journals (Sweden)

    Yuzun Fan

    2012-01-01

    Full Text Available Pure phase anatase TiO2 nanoparticles with sizes of 5–8 nm and varying crystallinity were synthesized in supercritical isopropanol/water using a continuous flow reactor. Their photodegradation of rhodamine B (RhB was evaluated under visible light irradiation. The as-prepared TiO2 nanoparticles show much higher photodegradation efficiencies than commercial Degussa P25 TiO2. Moreover, the photodegradation of RhB on the as-prepared TiO2 follows a different process from that on P25 TiO2, quicker N-deethylation and slower cleavage of conjugated chromophore structure. Based on PXRD, TEM, and BET measurements, these two photodegradation properties have been explained by the physicochemical properties of TiO2.

  11. Marine sponge skeleton photosensitized by copper phthalocyanine: A catalyst for Rhodamine B degradation

    Directory of Open Access Journals (Sweden)

    Norman Małgorzata

    2016-01-01

    Full Text Available We present a combined approach to photo-assisted degradation processes, in which a catalyst, H2O2 and UV irradiation are used together to enhance the oxidation of Rhodamine B (RB. The heterogeneous photocatalyst was made by the process of adsorption of copper phthalocyanine tetrasulfonic acid (CuPC onto purified spongin-based Hippospongia communis marine sponge skeleton (HcS. The product obtained, CuPC-HcS, was investigated by a variety of spectroscopic (carbon-13 nuclear magnetic resonance 13C NMR, Fourier transform infrared spectroscopy FTIR, energy-dispersive X-ray spectroscopy EDS and microscopic techniques (scanning electron microscopy SEM, fluorescent and optical microscopy, as well as thermal analysis. The study confirms the stable combination of the adsorbent and adsorbate. For a 10 mg/L RB solution, the percentage degradation reached 95% using CuPC-HcS as a heterocatalyst. The mechanism of RB removal involves adsorption and photodegradation simultaneously.

  12. A new rhodamine-based fluorescent probe for the discrimination of Fe"3"+ from Fe"2"+

    International Nuclear Information System (INIS)

    You, Qi Hua; Huang, Hua Bin; Zhuang, Zhi Xia; Wang, Xiao Ru; Chan, Wing Hong

    2016-01-01

    A new rhodamine-based fluorescent probe for the discrimination of Fe"3"+ from Fe"2"+ has been designed and investigated. The probe shows an immediate visual color change in response to Fe"3"+ and Cu"2"+, while only Fe"3"+ triggers the fluorescent change of the probe. The existence of large amount of other metal ions shows negligible interference in the detection of Fe"3"+. The association constant K_a_s_s of 4.64 × 10"8 M"-"2 (R"2 = 0.994) and 5.38 × 10"8 M"-"2 (R"2 = 0.991) of the complex was derived from UV/Vis and fluorescence titration assuming 1:2 stoichiometry of probe–Fe"3"+ complex, respectively

  13. Degradation and removal of Ceftriaxone sodium in aquatic environment with Bi2WO6/g-C3N4 photocatalyst.

    Science.gov (United States)

    Zhao, Yanyan; Liang, Xuhua; Wang, Yongbo; Shi, Huanxian; Liu, Enzhou; Fan, Jun; Hu, Xiaoyun

    2018-08-01

    Modern chemistry aims to identify outstanding photocatalytic materials for antibiotic degradation given the overuse and misuse of antibiotics. Herein, highly efficient heterojunction photocatalysts composed of Bi 2 WO 6 nanoflowers (BW) and g-C 3 N 4 nanosheets (CNNs) were successfully synthesized. The Bi 2 WO 6 /g-C 3 N 4 (BW/CNNs) were presented for Ceftriaxone sodium degradation in the aquatic environment at low concentrations under visible light irradiation. The 40%-BW/CNNs showed excellent photocatalytic activity, and approximately 94.50% Ceftriaxone sodium was degraded under visible lightirradiation for 120 min. h + and O 2 - played major roles compared with ·OH in the photocatalytic process. The degradation mechanism and the intermediate products were proposed to better understand the reaction process. Moreover, the as-prepared photocatalysts were highly stable in recycling photocatalytic experiments. Therefore, the photocatalysts prepared in the study showed outstanding photocatalytic activity and potential applications in inhibiting environmental pollution. In addition, this work provides a new insight for constructing other high-performance, low-cost photocatalysts for wastewater treatment. Copyright © 2018 Elsevier Inc. All rights reserved.

  14. Two sugar-rhodamine "turn-on" fluorescent probes for the selective detection of Fe3 +

    Science.gov (United States)

    Chen, Qing; Fang, Zhijie

    2018-03-01

    Two new sugar-rhodamine fluorescent probes (RDG1 and RDG2) have been synthesized and characterized by 1H NMR, 13C NMR and HRMS. Their UV-Vis, fluorescence spectra and fluorescence-response to Fe3 + are investigated and discussed. RDG1 had a very nice linear relationship between UV absorbance and Fe3 + concentration with the correlation coefficient as high as 0.997 and the detection limit is 3.46 × 10- 6 M. Upon the addition of Fe3 +, the spirolactam ring of RDG1 was opened and a 1:1 metal ligand complex was formed from Job's plot. The results showed that RDG1 can be used as an effective fluorescent probe for selective detection of Fe3 + in water. RDG2 was incorporated the well-known rhodamine group and a water-soluble D-glucose group within one molecule and can be used for detecting Fe3 + in natural water as a selective fluorescent sensor. The addition of Fe3 + into RDG2 resulted in a strongly enhanced fluorescence as well as color change of solution from colorless to pink. Job's plot of RDG2 indicated 1:1 stoichiometry of RDG2-Fe3 +. RDG2 can serve as a probe for Fe3 + between pH = 4.0 to 7.0 and it's detection limit is 2.09 × 10- 6 M. The OFF-ON fluorescent mechanisms of RDG1-Fe3 + and RDG2-Fe3 + are proposed.

  15. Biochemical study of some environmental pollutants dyes Part II: disperse dyes

    International Nuclear Information System (INIS)

    Shakra, S.; Ahmed, F.A.; Fetyan, N.A.

    2005-01-01

    This work was aimed to develop a method for removal of the dyes color from the textile wastewater that is well be much less costly than the other chemical or physical methods used. It therefore included: 1. Preparation of three disperses dyes. 2. Isolation of dyes degradable microorganisms from wastewater effluents and soil after adding 200 ppm of each dye individually. 3. Decolorisation and biodegradation of the dyes in liquid culture of the isolated bacteria (Bacillus thuringiensis). 4. Identification of the probable byproducts by different instruments. 5. Toxicity assessment of the dyes and their biodegraded products

  16. Degradation of organic dyes using spray deposited nanocrystalline stratified WO3/TiO2 photoelectrodes under sunlight illumination

    Science.gov (United States)

    Hunge, Y. M.; Yadav, A. A.; Mahadik, M. A.; Bulakhe, R. N.; Shim, J. J.; Mathe, V. L.; Bhosale, C. H.

    2018-02-01

    The need to utilize TiO2 based metal oxide hetero nanostructures for the degradation of environmental pollutants like Rhodamine B and reactive red 152 from the wastewater using stratified WO3/TiO2 catalyst under sunlight illumination. WO3, TiO2 and stratified WO3/TiO2 catalysts were prepared by a spray pyrolysis method. It was found that the stratified WO3/TiO2 heterostructure has high crystallinity, no mixed phase formation occurs, strong optical absorption in the visible region of the solar spectrum, and large surface area. The photocatalytic activity was tested for degradation of Rhodamine B (Rh B) and reactive red 152 in an aqueous medium. TiO2 layer in stratified WO3/TiO2 catalyst helps to extend its absorption spectrum in the solar light region. Rh B and Reactive red 152is eliminated up to 98 and 94% within the 30 and 40 min respectively at optimum experimental condition by stratified WO3/TiO2. Moreover, stratified WO3/TiO2 photoelectrode has good stability and reusability than individual TiO2 and WO3 thin film in the degradation of Rh B and reactive red 152. The photoelectrocatalytic experimental results indicate that stratified WO3/TiO2 photoelectrode is a promising material for dye removal.

  17. Synthesis, characterization and dyeing behavior of heterocyclic acid dyes and mordent acid dyes on wool and silk fabrics

    Directory of Open Access Journals (Sweden)

    Patel Hitendra M.

    2012-01-01

    Full Text Available Novel heterocyclic acid and mordent acid dyes were synthesized by the coupling of diazonium salt solution of different aromatic amines with 2- butyl-3-(4-hydroxybenzoylbenzofuran. The resulting heterocyclic acid dyes were characterized by spectral techniques, i.e., elemental analysis, IR, 1HNMR, 13C-NMR spectral studies and UV- visible spectroscopy. The dyeing performance of all the heterocyclic acid dyes was evaluated on wool and silk fabrics. The dyeing of chrome pre treated wool and silk fabrics showed better hues on mordented fabrics. Dyeing of wool and silk fabrics resulted in pinkish blue to red shades with very good depth and levelness. The dyed fabrics showed excellent to very good light, washing, perspiration, sublimation and rubbing fastness.

  18. Fate of Colored Smoke Dyes

    Science.gov (United States)

    1992-01-01

    4.13] have been applied to their estimation. This approach has the advantages of sensitivity and of not requiring high purity and known structures...Chrom absorbance detector, and an Alltech Econosil C-18 (10 micrometer) column (4.6 mm X 25 cm with guard column). The mobile phase, HPLC-grade methanol...water partition coefficient or vice versa. The HPLC method is of similar precision and has the advantage that known structure and purity of the dye are

  19. Broad band exciplex dye lasers

    International Nuclear Information System (INIS)

    Dienes, A.; Shank, C.V.; Trozzolo, A.M.

    1975-01-01

    The disclosure is concerned with exciplex dye lasers, i.e., lasers in which the emitting species is a complex formed only from a constituent in an electronically excited state. Noting that an exciplex laser, favorable from the standpoint of broad tunability, results from a broad shift in the peak emission wavelength for the exciplex relative to the unreacted species, a desirable class resulting in such broad shift is described. Preferred classes of laser media utilizing specified resonant molecules are set forth. (auth)

  20. Dataset on analysis of dyeing property of natural dye from Thespesia populnea bark on different fabrics

    Directory of Open Access Journals (Sweden)

    Kuchekar Mohini

    2018-02-01

    Full Text Available The natural dyes separated from plants are of gaining interest as substitutes for synthetic dyes in food and cosmetics. Thespesia populnea (T. populnea is widely grown plant and used in the treatment of various diseases. This study was aimed to separate natural dye from T. populnea bark and analysis of its dyeing property on different fabrics. In this investigation pharmacognostic study was carried out. The pharmacognostic study includes morphological study, microscopical examination, proximate analysis along with the phytochemical study. The dyeing of different fabric was done with a natural dye extracted from T. populnea bark. The fabrics like cotton, butter crep, polymer, chiken, lone, ulene and tarakasa were dye with plant extract. The various evaluation parameters were studied. It includes effect of washing with water, effect of soap, effect of sunlight, effect of alum, effect of Cupric sulphate, microscopical study of fabrics and visual analysis of dyeing by common people were studied. In results, natural dye isolated from T. populnea bark could be used for dyeing fabrics with good fastness properties. The studies reveals that, the dyeing property of fabrics after washing with water and soap, exposed to sunlight does not get affected. It was observed that cotton and tarakasa stains better as compared with other fabrics. It was concluded that the ethanolic extract having good dyeing property. Keywords: Plant, Thespesia populnea, Bark, Natural dye, Fabrics

  1. ICE-6G models of postglacial relative sea-level history applied to Holocene coral reef and mangrove records of the western Caribbean

    Science.gov (United States)

    Toscano, M. A.; Peltier, W. R.; Drummond, R.; Gonzalez, J.

    2012-12-01

    Fossil coral reefs and mangrove peat accumulations at western Caribbean sites along a latitudinal gradient from the Florida Keys through Belize and Panama provide dated and interpreted 8,000 year Holocene sea-level records for comparison with RSL predictions of the ICE-6G (VM5A, VM5B; L90) models of glacio-hydro-isostatic adjustment, with and without rotational feedback. These presumably passive continental margin sites provide the means to establish a N-S spatial trend in the varying influences of GIA, eustatic components of Holocene sea level, extent of forebulge collapse and influence of rotational feedback over a 20° latitudinal range. Previous ICE6G (VM5A) model-coral data comparisons for St Croix, USVI, Antigua, Martinique and Barbados (Toscano, Peltier and Drummond, 2011, QSR) along the eastern Caribbean plate and island arc illustrated the close model-data compatibility, the influence of rotational feedback acting as a significant factor in reducing misfits, and the need for high quality in situ data to confirm the extension of the proglacial forebulge into tropical latitudes. The gradient of western Caribbean continental shelf sites comprises a much more varied range of model-data relationships based on extensive combined Acropora palmata (reef crest coral) and Rhizophora mangle (microtidal mangrove) peat datasets in all cases. Starting at the northernmost region with the Florida Keys, there exist negative model misfits to the data, suggesting the possibility of a positive tectonic overprint upon expectations related to the glacial isostatic adjustment process acting alone, even though this region is normally believed to be tectonically stable. The largest multi-proxy database from Belize supports the likelihood of increasing rates of subsidence from north to south in the Belize Lagoon, which may account for numerous positive GIA model-data misfits. The southernmost site at Panama is most similar to Belize in the possible nature of tectonic influences on

  2. Effect of temperature on the expansion and microstructure Of U3 Si2-AI mini plate fuel of 3.6 g/cm3 uranium loading

    International Nuclear Information System (INIS)

    Ginting, A. Br.; Samosir, N.; Suparjo; Nasution, H.

    2000-01-01

    Expansion analysis has been conducted to 50 x 20-mm U 3 Si 2 -AI mini plate of 3.6 g/cm 3 uranium loading using dilatometer. The analysis was carried out at various temperatures of 170 o C, 350 o C and 550 o C in Argon medium with delay time 4 days. The result showed that the fuel plate was relatively stable with increasing of heating time but underwent significant expansion. Heating at 170 o C, 350 o C and 550 o C resulted in the expansion of the U 3 Si 2 -AI fuel plate of to 83-212 mum, 333-475 mum, and 433-724 mum with coefficient expansion of 24.2x10 -6 / o C - 24.3x10 -6 / o C, 25.5x10 -6 / o C - 26.2x10 -6 /'oC and 26.6 x 10 -6 / o C - 28.2 x 10 -6 / o C respectively. Microanalysis of the U 3 Si 2 -AI mini plate fuel with SEM-EDS upon heating at those temperature variation showed that microstructure change didn't occur at 170 o C, mean while interaction between AIMg2 cladding and the fuel meat appeared to take place at 350 o C and 550 o C. Data on the expansion and microstructure change of U 3 Si 2 -AI fuel plate upon heating are of great important for the manufacture/fabrication of research fuel plate to produce silicide fuel element for higher uranium loading. (author)

  3. Glacial Isostatic Adjustment Derived Boundary Conditions for Paleoclimate Simulation: the Refined ICE-6G_D (VM5a) Model and the Dansgaard-Oeschger Oscillation

    Science.gov (United States)

    Peltier, W. R.; Vettoretti, G.; Argus, D. F.

    2017-12-01

    Global models of the glacial isostatic adjustment (GIA) process are designed to fit a wide range of geophysical and geomorphological observations that simultaneously constrain the internal viscoelastic structure of Earths interior and the history of grounded ice thickness variations that has occurred over the most recent ice-age cycle of the Late Quaternary interval of time. The most recent refinement of the ICE-NG (VMX) series of such global models from the University of Toronto, ICE-6G_C (VM5a), has recently been slightly modified insofar as its Antarctic component is concerned to produce a "_D" version of the structure. This has been chosen to provide the boundary conditions for the next round of model-data inter-comparisons in the context of the international Paleoclimate Modeling Inter-comparison Project (PMIP). The output of PMIP will contribute to the Sixth Assessment Report (AR6) of the Intergovernmental Panel on Climate Change which is now under way. A highly significant test of the utility of this latest model has recently been performed that is focused upon the Dansgaard-Oeschger oscillation that was the primary source of climate variability during Marine Isotope Stage 3 (MIS3) of the most recent glacial cycle. By introducing the surface boundary conditions for paleotopography and paleobathymetry, land-sea mask and surface albedo into the NCAR CESM1 coupled climate model configured at full one degree by one degree CMIP5 resolution, together with the appropriate trace gas and orbital insolation forcing, we show that the millennium timescale Dansgard-Oeschger oscillation naturally develops following spin- up of the model into the glacial state.

  4. Preparation of carbon microspheres decorated with silver nanoparticles and their ability to remove dyes from aqueous solution

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Qingchun [Department of Chemistry, Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai 200092 (China); Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433 (China); Wu, Qingsheng, E-mail: qswu@tongji.edu.cn [Department of Chemistry, Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai 200092 (China); Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433 (China)

    2015-02-11

    Highlights: • Carbon microspheres decorated with silver nanoparticles (AgNP-CMSs) were prepared. • AgNP-CMSs show not only rapid and high adsorption capacity to methylene blue (MB) in water, but also excellent reusability. • It exhibits photocatalytic activity to Rhodamine B as well as MB under visible light. • The adsorption is from the ionic interactions but not the π–π conjugations. • The origin of photocatalysis is a surface plasmon resonance effect of AgNP on CMSs. - Abstract: Solid, but not hollow or porous, carbon microspheres decorated with silver nanoparticles (AgNP-CMSs) were prepared from silver nitrate and CMSs by a redox reaction at room temperature. The CMSs and AgNP-CMSs were characterized using X-ray diffraction, scanning electron microscopy, field emission scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), and UV–vis spectrophotometry. Though with non-high specific surface area, the AgNP-CMSs exhibited a high adsorption capacity toward methylene blue (MB) in an aqueous solution. The AgNP-CMSs were able to remove all the MB from a solution of 30 mg/L MB in water within 1 min when the adsorbent concentration was 0.12 g/L. The AgNP-CMSs also exhibited good adsorption and photocatalytic activity in the decomposition of aqueous Rhodamine B as well as MB under visible light. FTIR was used to examine the interaction between AgNP-CMSs and MB, and the spectrum and more extra experiments suggest ionic interactions between cationic dyes and the negatively charged groups can be formed but not the presence of abundant π–π conjugations between dye molecules and the aromatic rings. The origin of the photocatalytic activity of AgNP-CMSs was attributed to a surface plasmon resonance (SPR) effect of the silver nanoparticles on the CMSs.

  5. Synthesis and characterization of reactive dye-cassava mesocarp ...

    African Journals Online (AJOL)

    The synthesis of triazine based reactive dyes was carried out. The resultant dyes were characterized by thin layers chromatography, molecular weight, infrared and ultra- violet spectroscopy, and used in dyeing cassava mesocarp to produce dye modified cellulosic substrates. The dyed substrates were tested for dye fixation, ...

  6. Dye removal from textile wastewater using bioadsorbent

    International Nuclear Information System (INIS)

    Gardazi, S.M.H.

    2014-01-01

    Textile industries throughout the world produce huge quantities of dyes and pigments annually. Effluents from textile industries are dye wastewater, and disposal of these wastes to freshwater bodies causes damage to the environment. Among the treatment technologies, adsorption is an attractive and viable option, provided that the sorbent is inexpensive and readily available for use. In this study, a typical basic dye, methylene blue, in wastewater was treated using Melia azedarach sawdust. The effects of contact time, adsorbent amount and particle size were investigated on the removal efficiency of adsorbent for methylene blue. Complete removal of the dye were attained at higher adsorbent dose of 3 g/L with 50 mg/L initial dye concentration. The maximum adsorption was at 240 minutes, whereas more than 90% removal with 105 meu m particle size of 1 g/L adsorbent for same initial dye concentration. The experimental data best fits with 2 Langmuir adsorption isotherm (R= 0.991). (author)

  7. Biological wastewater treatment of azo dyes

    Energy Technology Data Exchange (ETDEWEB)

    Shaul, G.M.; Dempsey, C.R.; Dostal, K.A. (Environmental Protection Agency, Cincinnati, OH (USA))

    1988-09-01

    EPA Water Engineering Research Laboratory, Office of Research and Development, undertook a study to determine the fate of specific water soluble azo dye compounds in the activated sludge process (ASP). The study was approached by dosing the feed to the pilot ASP systems with various water soluble azo dyes and by monitoring each dye compound through the system, analyzing both liquid and sludge samples. The fate of the parent dye compound was assessed via mass balance calculations. These data could determine if the compound was removed by adsorption, apparent biodegradation, or not removed at all. The paper presents results for 18 dye compounds tested from June 1985 through August 1987. The study was conducted at EPAs Test and Evaluation Facility in Cincinnati, Ohio. The objective of this study was to determine the partitioning of water soluble azo dyes in the ASP.

  8. Panchromatic Response in Solid-State Dye-Sensitized Solar Cells Containing Phosphorescent Energy Relay Dyes

    KAUST Repository

    Yum, Jun-Ho; Hardin, Brianâ E.; Moon, Soo-Jin; Baranoff, Etienne; Nà ¼ esch, Frank; McGehee, Michaelâ D.; Grà ¤ tzel, Michael; Nazeeruddin, Mohammadâ K.

    2009-01-01

    Running relay: Incorporating an energyrelay dye (ERD) into the hole transporter of a dye-sensitized solar cell increased power-conversion efficiency by 29% by extending light harvesting into the blue region. In the operating mechanism (see picture

  9. Time dependent – density functional theory characterization of organic dyes for dye-sensitized solar cells

    KAUST Repository

    Hilal, Rifaat; Aziz, Saadullah G.; Osman, Osman I.; Bredas, Jean-Luc

    2017-01-01

    We aim at providing better insight into the parameters that govern the intramolecular charge transfer (ICT) and photo-injection processes in dyes for dye-sensitised solar cells (DSSC). Density functional theory (DFT) and time-dependent DFT (TD

  10. Incorporating Multiple Energy Relay Dyes in Liquid Dye-Sensitized Solar Cells

    KAUST Repository

    Yum, Jun-Ho; Hardin, Brian E.; Hoke, Eric T.; Baranoff, Etienne; Zakeeruddin, Shaik M.; Nazeeruddin, Mohammad K.; Torres, Tomas; McGehee, Michael D.; Grä tzel, Michael

    2011-01-01

    Panchromatic response is essential to increase the light-harvesting efficiency in solar conversion systems. Herein we show increased light harvesting from using multiple energy relay dyes inside dye-sensitized solar cells. Additional photoresponse

  11. Increased light harvesting in dye-sensitized solar cells with energy relay dyes

    KAUST Repository

    Hardin, Brian E.; Hoke, Eric T.; Armstrong, Paul B.; Yum, Jun-Ho; Comte, Pascal; Torres, Tomá s; Fré chet, Jean M. J.; Nazeeruddin, Md Khaja; Grä tzel, Michael; McGehee, Michael D.

    2009-01-01

    Conventional dye-sensitized solar cells have excellent charge collection efficiencies, high open-circuit voltages and good fill factors. However, dye-sensitized solar cells do not completely absorb all of the photons from the visible and near

  12. Increased light harvesting in dye-sensitized solar cells with energy relay dyes

    KAUST Repository

    Hardin, Brian E.

    2009-06-21

    Conventional dye-sensitized solar cells have excellent charge collection efficiencies, high open-circuit voltages and good fill factors. However, dye-sensitized solar cells do not completely absorb all of the photons from the visible and near-infrared domain and consequently have lower short-circuit photocurrent densities than inorganic photovoltaic devices. Here, we present a new design where high-energy photons are absorbed by highly photoluminescent chromophores unattached to the titania and undergo Förster resonant energy transfer to the sensitizing dye. This novel architecture allows for broader spectral absorption, an increase in dye loading, and relaxes the design requirements for the sensitizing dye. We demonstrate a 26% increase in power conversion efficiency when using an energy relay dye (PTCDI) with an organic sensitizing dye (TT1). We estimate the average excitation transfer efficiency in this system to be at least 47%. This system offers a viable pathway to develop more efficient dye-sensitized solar cells.

  13. Hair dye poisoning and the developing world

    Directory of Open Access Journals (Sweden)

    Sampathkumar Krishnaswamy

    2009-01-01

    Full Text Available Hair dye poisoning has been emerging as one of the important causes of intentional self harm in the developing world. Hair dyes contain paraphenylene-diamine and a host of other chemicals that can cause rhabdomyolysis, laryngeal edema, severe metabolic acidosis and acute renal failure. Intervention at the right time has been shown to improve the outcome. In this article, we review the various manifestations, clinical features and treatment modalities for hair dye poisoning.

  14. Synthesis, characterization of organo-modified zeolitic nanomaterial from coal ash and application as adsorbent on remediation of contaminated water by rhodamine B and direct blue 71

    International Nuclear Information System (INIS)

    Alcântara, Raquel Reis

    2016-01-01

    The synthesis of zeolites from mineral coal fly and bottom ash was performed by alkaline hydrothermal treatment, which were named ZFA and ZBA, respectively. Organo-modified zeolites, SMZF and SMZB, were obtained from surface modification of ZFA and ZBA, respectively, using the cationic surfactant hexadecyltrimethylammonium bromide. From the remaining solutions generated in ZFA and ZBA synthesis it was possible to synthesis two new zeolites. The physicochemical characteristics of the synthesized nanomaterials zeolite as well as their respective raw materials, such as cation exchange capacity, density, specific area, chemical composition, mineralogical and morphological, among others, were determined. The adsorbents SMZF and SMZB were used to remove the dyes, Direct Blue 71 (DB71) and Rhodamine B (RB) from aqueous solutions in batch system. Thus, four systems DB71/SMZF, RB/SMZF, DB71/SMZB, RB/SMZB were investigated. The models of pseudo-first order and pseudo-second order were applied to the experimental data for the study the adsorption kinetics. The model of pseudo-second order was the one that best described the adsorption of all dye/organomodified-zeolites systems. The equilibrium adsorption was analyzed from four models isotherm, namely: Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D-B). The results show that the model Freundlich and Langmuir best described the experimental data systems DB71/SMZF and DB71/SMZB, respectively. For systems with RB, the model D-R was the best fit for both adsorbents (SMZF and SMZB). The factorial design 2 4 was applied to the analysis of the following factors influencing the adsorption process: initial concentration of dye (C o ), pH, amount of adsorbent (M) and temperature (T). Under the conditions studied it concludes with the confidence interval of 95%, which for the DB71/SMZF system, the factors and their interactions that influence more were C 0 , M, pH, pH∗M, pH∗C 0 , M∗C 0 , pH∗M∗C 0 , in that order. In DB

  15. Solubilization of Hydrophobic Dyes in Surfactant Solutions

    Directory of Open Access Journals (Sweden)

    Ali Reza Tehrani-Bagha

    2013-02-01

    Full Text Available In this paper, the use of surfactants for solubilization of hydrophobic organic dyes (mainly solvent and disperse dyes has been reviewed. The effect of parameters such as the chemical structures of the surfactant and the dye, addition of salt and of polyelectrolytes, pH, and temperature on dye solubilization has been discussed. Surfactant self-assemble into micelles in aqueous solution and below the concentration where this occurs—the critical micelle concentration (CMC—there is no solubilization. Above the CMC, the amount of solubilized dye increases linearly with the increase in surfactant concentration. It is demonstrated that different surfactants work best for different dyes. In general, nonionic surfactants have higher solubilization power than anionic and cationic surfactants. It is likely that the reason for the good performance of nonionic surfactants is that they allow dyes to be accommodated not only in the inner, hydrocarbon part of the micelle but also in the headgroup shell. It is demonstrated that the location of a dye in a surfactant micelle can be assessed from the absorption spectrum of the dye-containing micellar solution.

  16. Ultrasonic assisted dyeing: dyeing of acrylic fabrics C.I. Astrazon Basic Red 5BL 200%.

    Science.gov (United States)

    Kamel, M M; Helmy, H M; Mashaly, H M; Kafafy, H H

    2010-01-01

    The dyeing of acrylic fabrics using C.I. Astrazon Basic Red 5BL 200% has been studied with both conventional and ultrasonic techniques. The effect of dye concentration, dye bath pH, ultrasonic power, dyeing time and temperature were studied and the resulting shades obtained by dyeing with both techniques were compared. Colour strength values obtained were found to be higher with ultrasonic than with conventional heating. The results of fastness properties of the dyed fabrics were studied. X-ray and Scanning Electron Microscope SEM were carried out on dyed samples using both methods of dyeing to find out an explanation for the better dyeability of acrylic fabrics with (US) method. Dyeing kinetics of acrylic fabrics using C.I. Astrazon Basic Red 5BL 200% using conventional and ultrasonic conditions were compared. The time/dye-uptake isotherms are revealing the enhanced dye-uptake in the second phase of dyeing. The values of dyeing rate constant, half-time of dyeing and standard affinity and ultrasonic efficiency have been calculated and discussed.

  17. High Excitation Transfer Efficiency from Energy Relay Dyes in Dye-Sensitized Solar Cells

    KAUST Repository

    Hardin, Brian E.; Yum, Jun-Ho; Hoke, Eric T.; Jun, Young Chul; Péchy, Peter; Torres, Tomás; Brongersma, Mark L.; Nazeeruddin, Md. Khaja; Grätzel, Michael; McGehee, Michael D.

    2010-01-01

    The energy relay dye, 4-(Dicyanomethylene)-2-methyl-6-(4- dimethylaminostyryl)-4H-pyran (DCM), was used with a near-infrared sensitizing dye, TT1, to increase the overall power conversion efficiency of a dye-sensitized solar cell (DSC) from 3

  18. Photostability of low cost dye-sensitized solar cells based on natural and synthetic dyes

    Science.gov (United States)

    Abdou, E. M.; Hafez, H. S.; Bakir, E.; Abdel-Mottaleb, M. S. A.

    2013-11-01

    This paper deals with the use of some natural pigments as well as synthetic dyes to act as sensitizers in dye-sensitized solar cells (DSSCs). Anthocyanin dye extracted from rosella (Hibiscus sabdariffa L.) flowers, the commercially available textile dye Remazole Red RB-133 (RR) and merocyanin-like dye based on 7-methyl coumarin are tested. The photostability of the three dyes is investigated under UV-Vis light exposure. The results show a relatively high stability of the three dyes. Moreover, the photostability of the solid dyes is studied over the TiO2 film electrodes. A very low decolorization rates are recorded as; rate constants k = 1.6, 2.1 and 1.9 × 10-3 min-1 for anthocyanin, RR and coumarin dyes, respectively. The stability results favor selecting anthocyanin as a promising sensitizer candidate in DSSCs based on natural products. Dyes-sensitized solar cells are fabricated and their conversion efficiency (η) is 0.27%, 0.14% and 0.001% for the anthocyanin, RR and coumarin dyes, respectively. Moreover, stability tests of the sealed cells based on anthocyanin and RR dyes are done under continuous light exposure of 100 mW cm-2, reveals highly stable DSSCs.

  19. Acid-base indicator properties of dyes from local plants I: Dyes from ...

    African Journals Online (AJOL)

    DR. MIKE HORSFALL

    Acid-base indicator properties of dyes from local plants I: Dyes from Basella alba. (Indian spinach) and ... solution, which change colour immediately after the equivalence point has .... The pH ranges over which the dyes change colour were ...

  20. A specific Tween-80-Rhodamine S-MWNTs phosphorescent reagent for the detection of trace calcitonin

    Energy Technology Data Exchange (ETDEWEB)

    Liu Jiaming, E-mail: zzsyliujiaming@163.com [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Huang Xiaomei; Zhang Lihong; Zheng Zhiyong [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou, 363000 (China); Lin Xuan; Zhang Xiaoyang; Jiao Li; Cui Malin [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Jiang Shulian [Fujian Provincial Bureau of Quality and Technical Supervision, Zhangzhou, 363000 (China); Lin Shaoqin [Department of Biochemistry, Fujian Education College, Fuzhou 350001 (China)

    2012-09-26

    Graphical abstract: A new Tween-80-Rhodamine S-water-soluble multi-walled carbon nanotubes (Tween-80-Rhod.S-MWNTs-EDC-NHS, TRMEN) phosphorescent labelling reagent was developed. High sensitive solid substrate room temperature phosphorescence immunoassay (SSRTPIA) for the determination of calcitonin (CT) in human serum and the prediction of human diseases based on the TRMEN could be used to label anti-calcitonin antibody (Ab{sub CT}) to form the TRMEN-Ab{sub CT} labelling product, which could take high specific immunoreaction with CT causing that the {Delta}I{sub p} of the system was linear to the content of CT. Moreover, the reaction mechanisms of both labelling Ab{sub CT} by TRMEN and SSRTPIA for the determination of trace CT were discussed. This research not only provides a new hormones analysis method, but also expands the application field of MWNTs and promotes the development of SSRTP and IA. --Highlights: Black-Right-Pointing-Pointer A Tween-80-Rhodamine S-multi-walled carbon nanotubes labelling reagent was developed. Black-Right-Pointing-Pointer The phosphorescence immunoassay was established for the determination of calcitonin. Black-Right-Pointing-Pointer This method has been applied to determine CT and the prediction of diseases. Black-Right-Pointing-Pointer The structure of MWNTs was characterized with SEM and IR. Black-Right-Pointing-Pointer The mechanisms for both determining trace CT and labelling Ab{sub CT} were discussed. - Abstract: The present study proposed a simple sensitive and specific immunoassay for the quantification of calcitonin (CT) in human serum with water-soluble multi-walled carbon nanotubes (MWNTs). The -COOH group of MWNTs could react with the -NH- group of rhodamine S (Rhod.S) molecules to form Rhod.S-MWNTs, which could emit room temperature phosphorescence (RTP) on acetate cellulose membrane (ACM) and react with Tween-80 to form micellar compound. Tween-80-Rhod.S-MWNTs (TRM), as a phosphorescent labelling reagent, could

  1. Aerobic decolourization of two reactive azo dyes under varying ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-02-01

    Feb 1, 2010 ... Azo dyes generally resist aerobic microbial degra- dation, only organisms with specialized azo dye reducing enzymes were found to degrade azo dyes under fully aerobic ... textile mill, in sterile plastic bottles. Isolation of ...

  2. Mn2O3 decorated graphene nanosheet: An advanced material for the photocatalytic degradation of organic dyes

    International Nuclear Information System (INIS)

    Chandra, Sourov; Das, Pradip; Bag, Sourav; Bhar, Radhaballabh; Pramanik, Panchanan

    2012-01-01

    Graphical abstract: A facile and economical route has been developed for the synthesis of graphene–Mn 2 O 3 nanocomposite in which Mn 2 O 3 nanoparticles are uniformly distributed throughout the surface of the graphene nanosheet with their average sizes ranging from 8 to 10 nm. It shows a brilliant catalytic activity during the photodegradation of several organic dyes as compare to both of the bare manganese oxide and graphene too. Highlights: ► One step sonochemical synthesis of graphene–Mn 2 O 3 nanocomposite. ► Growth of such nanoparticles over graphene is accelerated by the simultaneous reduction with KMnO 4 . ► The composite can effectively use as heterogeneous catalyst during the photodegradation of organic dyes. ► It exhibits ∼84%, ∼80% and ∼60% degradation of MB, eosin and RB respectively within a few minutes. - Abstract: A one step sonochemical route has been developed to prepare graphene–Mn 2 O 3 nanocomposite with uniform distribution of Mn 2 O 3 nanoparticles throughout the surface of graphene nanosheet. Growth of such nanoparticles over this two dimensional carbon network is simply accelerated by the simultaneous reduction of potassium permanganate along with graphene oxide, in which metal ions are first anchored through binding with oxy-functional groups of graphene oxide and finally reduced by hydrazine. The final product ensure a new platform for the photodegradation of organic dyes, as it can store electrons and circulate them towards dye molecules through the formation of hydroxyl radical under the exposure of UV-light. Almost 80% photocatalytic degradation of eosin, methylene blue and rhodamine B have been observed within few minutes, which has not been obtained by using bare manganese oxide itself.

  3. Synthesis and Characterization of Reduced Graphene Oxide/Rhodamine 101 (rGO-Rh101) Nanocomposites and Their Heterojunction Performance in rGO-Rh101/ p-Si Device Configuration

    Science.gov (United States)

    Batır, G. Güven; Arık, Mustafa; Caldıran, Zakir; Turut, Abdulmecit; Aydogan, Sakir

    2018-01-01

    Reduced graphene oxide (rGO)-rhodamine 101 (Rh101) nanocomposites with different ratios of rGO have been synthesized in aqueous medium by ultrasonic homogenization. The fluorescence of Rh101 as measured using a laser dye with high fluorescence quantum yield was substantially quenched with increasing amount of rGO in the nanocomposite. Formation of rGO-Rh101 nanocomposites was confirmed by x-ray diffraction analysis, scanning electron microscopy, ultraviolet-visible (UV-Vis) spectroscopy, and fluorescence microscopy. Furthermore, rGO-Rh101 nanocomposite/ p-Si heterojunctions were synthesized, all of which showed good rectifying behavior. The electrical characteristics of these devices were analyzed using current-voltage ( I- V) measurements to determine the ideality factor and barrier height. The experimental results confirmed the presence of lateral inhomogeneity in the effective barrier height of the rGO-Rh101 nanocomposite/ p-Si heterojunctions. In addition to I- V measurements, one device was analyzed in more detail using frequency-dependent capacitance-voltage measurements. All electrical measurements were carried out at room temperature and in the dark.

  4. Dye Sensitized Solar Cell, DSSC

    Directory of Open Access Journals (Sweden)

    Pongsatorn Amornpitoksuk

    2003-07-01

    Full Text Available A dye sensitized solar cell is a new type of solar cell. The operating system of this solar cell type is similar to plant’s photosynthesis process. The sensitizer is available for absorption light and transfer electrons to nanocrystalline metal oxide semiconductor. The ruthenium(II complexes with polypyridyl ligands are usually used as the sensitizers in solar cell. At the present time, the complex of [Ru(2,2',2'’-(COOH3- terpy(NCS3] is the most efficient sensitizer. The total photon to current conversion efficiency was approximately 10% at AM = 1.5.

  5. Development of a high-throughput liquid state assay for lipase activity using natural substrates and rhodamine B.

    Science.gov (United States)

    Zottig, Ximena; Meddeb-Mouelhi, Fatma; Beauregard, Marc

    2016-03-01

    A fluorescence-based assay for the determination of lipase activity using rhodamine B as an indicator, and natural substrates such as olive oil, is described. It is based on the use of a rhodamine B-natural substrate emulsion in liquid state, which is advantageous over agar plate assays. This high-throughput method is simple and rapid and can be automated, making it suitable for screening and metagenomics application. Reaction conditions such as pH and temperature can be varied and controlled. Using triolein or olive oil as a natural substrate allows monitoring of lipase activity in reaction conditions that are closer to those used in industrial settings. The described method is sensitive over a wide range of product concentrations and offers good reproducibility. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Random laser emission from a Rhodamine B-doped GPTS/TEOS-derived organic/silica monolithic xerogel

    Science.gov (United States)

    Abegão, Luis M. G.; Manoel, D. S.; Otuka, A. J. G.; Ferreira, P. H. D.; Vollet, D. R.; Donatti, D. A.; De Boni, L.; Mendonça, C. R.; De Vicente, F. S.; Rodrigues, J. J., Jr.; Alencar, M. A. R. C.

    2017-06-01

    A Rhodamine B-doped 3-glycidoxypropyltrimethoxysilane (GPTS)/tetraethyl orthosilicate (TEOS)-derived organic/silica monolithic xerogel with excellent optical properties was prepared and its potential as a random laser host investigated. This hybrid material has a non-porous organic/inorganic morphology with silica-rich nanoparticles of less than 10 nm in diameter homogeneously dispersed within the matrix. Random laser emission with incoherent feedback, centered at 618 nm, was observed from Rhodamine B incorporated into the monolithic xerogel when excited by a 532 nm pulsed laser. This hybrid system is shown to be very promising for the development of a new class of random laser-based integrated devices, with applications ranging from optical bio-imaging to sensing.

  7. Mixed ZnO-TiO2 Suspended Solution as an Efficient Photocatalyst for Decolonization of a Textile Dye from Waste Water

    Directory of Open Access Journals (Sweden)

    Mohammad Ali Mooji

    2015-03-01

    Full Text Available Introduction: Textile industries produce large volume of colored dye effluents which are toxic and removal of dyes from wastewater is a significant environmental issue. Advanced oxidation process (AOPs is alternative method for the complete degradation many organic pollutants. ZnO and TiO2 are important photocatalysts with high catalytic activity that have attracted much research attention. Material and Methods: Mixed ZnO/TiO2 was prepared with mixing of ZnO and TiO2 (20, 40, 60, 80 % (w/w. 20 mL of dye solution (80 mgL-1 for DB71 containing the appropriate quantity of photocatalyst was magnetically stirred under UV irradiation. Photocatalytic study was carried out to evaluate the effect of UV (400 W, ZnO/TiO2 weight percent (20, 40, 60, 80 % (w/w, pH (2.3 – 9.2, irradiation time of (10 – 70 min, initial dye concentration of (10, 40, 80 mg/L and ZnO/TiO2 dosage of (0.2 – 1.6 g/L on removal of dye. Dye concentration was monitored spectrophotometrically by measuring the dye absorbance at 285 nm. Results: In comparison with TiO2 or ZnO as photocatalyst, mixed photocatalyst (ZnO/TiO2 is more efficient catalyst for degradation of dye under UV irradiation Results show that approximately 90 % of Direct Blue 71 has been eliminated after 70 minutes and optimized condition ((pH = 6.4, ZnO/TiO2 (50% w/w, 1.25 g/L. Experiments showed, the noticeable decolorization of dye solution can be done without any oxidation agent with mixed ZnO/TiO2 photocatalyst.

  8. Incorporating Multiple Energy Relay Dyes in Liquid Dye-Sensitized Solar Cells

    KAUST Repository

    Yum, Jun-Ho

    2011-01-05

    Panchromatic response is essential to increase the light-harvesting efficiency in solar conversion systems. Herein we show increased light harvesting from using multiple energy relay dyes inside dye-sensitized solar cells. Additional photoresponse from 400-590 nm matching the optical window of the zinc phthalocyanine sensitizer was observed due to Förster resonance energy transfer (FRET) from the two energy relay dyes to the sensitizing dye. The complementary absorption spectra of the energy relay dyes and high excitation transfer efficiencies result in a 35% increase in photovoltaic performance. © 2011 Wiley-VCH Verlag GmbH& Co. KGaA.

  9. Solvent extraction of W(VI) and Hg(II) with malachite green and rhodamine-B respectively into organic solvents

    International Nuclear Information System (INIS)

    Patil, V.B.; David, M.M.; Turel, Z.R.

    1992-01-01

    Aqueous malachite green and alcoholic rhodamine-B have been used for the extraction of tungsten( W(VI)) and mercury( Hg(II)) respectively into nitrobenzene. This paper deals with developing a rapid method and selective method for the extraction of tungsten(W(VI)) and mercury (Hg(II)) using malachite green and rhodamine-B respectively. 185 W and 203 Hg were used as tracers for studying the extraction process.(author). 2 refs., 2 tab

  10. Rhodamine B triggers ovarian toxicity through oxidative stress, decreases in the number of follicles, 17B-estradiol level, and thickness of endometrium

    Directory of Open Access Journals (Sweden)

    Syiska Atik Maryanti

    2014-06-01

    Full Text Available Objective: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17beta-estradiol and thickness of endometrium. Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P 0.05. Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P 0.05. Administration of rhodamine B of the second and third doses in female rats can reduce 17-estradiol level significantly compared to the control (P 0.05. The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P 0.05. Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17-estradiol which ultimately lowered the thickness of endometrium. [Cukurova Med J 2014; 39(3.000: 451-457

  11. Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells

    Czech Academy of Sciences Publication Activity Database

    Cmiel, V.; Skopalík, J.; Poláková, K.; Solař, J.; Havrdová, M.; Milde, D.; Justan, I.; Magro, M.; Starčuk jr., Zenon; Provazník, I.

    2017-01-01

    Roč. 46, JUL (2017), s. 433-444 ISSN 0175-7571 R&D Projects: GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01 Institutional support: RVO:68081731 Keywords : intracellular fluorescent labels * stem cell tracking * dual contrast agents * iron oxide nanoparticles * confocal microscopy * mesenchymal stromal cells * rhodamine Subject RIV: FS - Medical Facilities ; Equipment OBOR OECD: Biophysics Impact factor: 1.472, year: 2016

  12. Characteristics of dye Rhoeo spathacea in dye sensitizer solar cell (DSSC)

    Science.gov (United States)

    Sumardiasih, Sri; Obina, Wilfrida M.; Cari; Supriyanto, Agus; Septiawan, Trio Y.; Khairuddin

    2017-01-01

    Dye-sensitized solar cell (DSSC) is a device that converts solar energy into electrical energy. The magnitude of the efficiency of DSSC is mainly based on the amount of dye absorbed by the surface of TiO2. In this work, used natural dye extracted from leaves Rhoeo spathacea. The dye partially used to immerse of TiO2 as working electrodes, and the rest are directly mixed TiO2 paste to obtain dye titanium dioxide.The paste TiO2 and dye titanium dioxide coated onto the fluorine-doped tin oxide (FTO) glass plate by spin coating method. The absorbance spectra of the dye, dye titanium dioxide and TiO2 were obtained by UV-Vis spectroscopy. The conductivity of the dye, dye titanium dioxide, and TiO2 was measured by two point probe El-Kahfi 100. The DSSC based on dye titanium dioxide that stirring for 5 hours the highest efficiency of 0,0520 % whereas those based on TiO2 immersed for 36 hours showed achieved 0,0501 % obtained from I-V characterization.

  13. Characteristics of dye Rhoeo spathacea in dye sensitizer solar cell (DSSC)

    International Nuclear Information System (INIS)

    Sumardiasih, Sri; Obina, Wilfrida M.; Cari; Supriyanto, Agus; Septiawan, Trio Y.; Khairuddin

    2017-01-01

    Dye-sensitized solar cell (DSSC) is a device that converts solar energy into electrical energy. The magnitude of the efficiency of DSSC is mainly based on the amount of dye absorbed by the surface of TiO 2 . In this work, used natural dye extracted from leaves Rhoeo spathacea. The dye partially used to immerse of TiO 2 as working electrodes, and the rest are directly mixed TiO 2 paste to obtain dye titanium dioxide.The paste TiO 2 and dye titanium dioxide coated onto the fluorine-doped tin oxide (FTO) glass plate by spin coating method. The absorbance spectra of the dye, dye titanium dioxide and TiO 2 were obtained by UV-Vis spectroscopy. The conductivity of the dye, dye titanium dioxide, and TiO 2 was measured by two point probe El-Kahfi 100. The DSSC based on dye titanium dioxide that stirring for 5 hours the highest efficiency of 0,0520 % whereas those based on TiO 2 immersed for 36 hours showed achieved 0,0501 % obtained from I-V characterization. (paper)

  14. Surface Behavior of Rhodamin and Tartrazine on Silica-Cellulose Sol-Gel Surfaces by Thin Layer Elution

    Directory of Open Access Journals (Sweden)

    Surjani Wonorahardjo

    2016-05-01

    Full Text Available Physical and chemical interactions are the principles for different types of separation systems as the equillibrium dynamics on surface plays a key-role. Surface modification is a way for selective separation at interfaces. Moreover, synthesis of gel silica by a sol-gel method is preferred due to the homogeneity and surface feature easily controlled. Cellulose can be added in situ to modified the silica features during the process. Further application for to study interaction of rhodamin and tartrazine in its surface and their solubilities in mobile phase explains the possibility for their separation. This paper devoted to evaluate the surface behavior in term of adsorption and desorption of tartrazine and rhodamin on silica-cellulose thin layer in different mobile phase. Some carrier liquids applied such as methanol, acetone, n-hexane and chloroform. The result proves tartrazine and rhodamin is separated and have different behavior in different mobile phase. The retardation factors (Rf of the mixtures suggest complexity behavior on silica-cellulose surface.

  15. Theoretical study of indoline dyes for dye-sensitized solar cells

    International Nuclear Information System (INIS)

    Ham, Ho Wan; Kim, Young Sik

    2010-01-01

    Indoline dye sensitizers were designed and studied theoretically to increase molar extinction coefficients in the visible to near infrared region for solar-cell devices. To gain insight into dye sensitizers' structural, electronic, and optical properties, DFT/TDDFT calculations were performed on a series of dye sensitizers derived from the D149. The good agreement between the experimental and TDDFT calculated absorption spectra of the D149 sensitizer allowed us to provide a detailed assessment of the main spectral features of a series of dye sensitizers. Increase in the conjugation length resulted in a more red-shifted spectral response and less positive oxidation potential than that of the D149. The dye with the dimethylfluorene group showed stronger absorption bands due to a large dipole moment. The calculated dipoles for the dye series correlate well with the observed strong absorption bands of the electronic spectra. These results provided useful clues for the molecular engineering of efficient organic dye sensitizers.

  16. High Excitation Transfer Efficiency from Energy Relay Dyes in Dye-Sensitized Solar Cells

    KAUST Repository

    Hardin, Brian E.

    2010-08-11

    The energy relay dye, 4-(Dicyanomethylene)-2-methyl-6-(4- dimethylaminostyryl)-4H-pyran (DCM), was used with a near-infrared sensitizing dye, TT1, to increase the overall power conversion efficiency of a dye-sensitized solar cell (DSC) from 3.5% to 4.5%. The unattached DCM dyes exhibit an average excitation transfer efficiency (EÌ?TE) of 96% inside TT1-covered, mesostructured TiO2 films. Further performance increases were limited by the solubility of DCM in an acetonitrile based electrolyte. This demonstration shows that energy relay dyes can be efficiently implemented in optimized dye-sensitized solar cells, but also highlights the need to design highly soluble energy relay dyes with high molar extinction coefficients. © 2010 American Chemical Society.

  17. Low-threshold conical microcavity dye lasers

    DEFF Research Database (Denmark)

    Grossmann, Tobias; Schleede, Simone; Hauser, Mario

    2010-01-01

    element simulations confirm that lasing occurs in whispering gallery modes which corresponds well to the measured multimode laser-emission. The effect of dye concentration on lasing threshold and lasing wavelength is investigated and can be explained using a standard dye laser model....

  18. Rhodamine Inhibitors of P-glycoprotein: An Amide/Thioamide “Switch” for ATPase Activity

    Science.gov (United States)

    Gannon, Michael K.; Holt, Jason J.; Bennett, Stephanie M.; Wetzel, Bryan R.; Loo, Tip W.; Bartlett, M. Claire; Clarke, David M.; Sawada, Geri A.; Higgins, J. William; Tombline, Gregory; Raub, Thomas J.; Detty, Michael R.

    2012-01-01

    We have examined 46 tetramethylrosamine/rhodamine derivatives with structural diversity in the heteroatom of the xanthylium core, the amino substituents of the 3- and 6-positions, and the alkyl, aryl, or heteroaryl group at the 9-substituent. These compounds were examined for affinity and ATPase stimulation in isolated MDR3 CL P-gp and human P-gp-His10, for their ability to promote uptake of calcein AM and vinblastine in multidrug-resistant MDCKII-MDR1 cells, and for transport in monolayers of MDCKII-MDR1 cells. Thioamide 31-S gave KM of 0.087 μM in human P-gp. Small changes in structure among this set of compounds affected affinity as well as transport rate (or flux) even though all derivatives examined were substrates for P-gp. With isolated protein, tertiary amide groups dictate high affinity and high stimulation while tertiary thioamide groups give high affinity and inhibition of ATPase activity. In MDCKII-MDR1 cells, the tertiary thioamide-containing derivatives promote uptake of calcein AM and have very slow passive, absorptive, and secretory rates of transport relative to transport rates for tertiary amide-containing derivatives. Thioamide 31-S promoted uptake of calcein AM and inhibited efflux of vinblastine with IC50’s of ~2 μM in MDCKII-MDR1 cells. PMID:19402665

  19. Occurrence of rhodamine B contamination in capsicum caused by agricultural materials during the vegetation process.

    Science.gov (United States)

    Gao, Wei; Wu, Naiying; Du, Jingjing; Zhou, Li; Lian, Yunhe; Wang, Lei; Liu, Dengshuai

    2016-08-15

    This paper reports on the environmental rhodamine B (RhB) contamination in capsicum caused by agricultural materials during the vegetation process. Ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was applied to detect 64 capsicum samples from China, Peru, India and Burma. Results demonstrated that RhB was found in all samples at low concentrations (0.11-0.98 μg/kg), indicating RhB contamination in capsicums is probably a ubiquitous phenomenon. In addition, studies into soils, roots, stems and leaves in Handan of Hebei province, China showed that the whole ecologic chain had been contaminated with RhB with the highest levels in leaves. The investigation into the agricultural environment in Handan of Hebei province and Korla of Xinjiang province, China demonstrated that the appearances of RhB contamination in the tested capsicums are mainly due to the agricultural materials contamination. The study verified that environmental contamination should be an important origin for the RhB contamination in capsicum fruits. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Bifunctional Rhodamine Probes of Myosin Regulatory Light Chain Orientation in Relaxed Skeletal Muscle Fibers

    Science.gov (United States)

    Brack, Andrew S.; Brandmeier, Birgit D.; Ferguson, Roisean E.; Criddle, Susan; Dale, Robert E.; Irving, Malcolm

    2004-01-01

    The orientation of the regulatory light chain (RLC) region of the myosin heads in relaxed skinned fibers from rabbit psoas muscle was investigated by polarized fluorescence from bifunctional rhodamine (BR) probes cross-linking pairs of cysteine residues introduced into the RLC. Pure 1:1 BR-RLC complexes were exchanged into single muscle fibers in EDTA rigor solution for 30 min at 30°C; ∼60% of the native RLC was removed and stoichiometrically replaced by BR-RLC, and >85% of the BR-RLC was located in the sarcomeric A-bands. The second- and fourth-rank order parameters of the orientation distributions of BR dipoles linking RLC cysteine pairs 100-108, 100-113, 108-113, and 104-115 were calculated from polarized fluorescence intensities, and used to determine the smoothest RLC orientation distribution—the maximum entropy distribution—consistent with the polarized fluorescence data. Maximum entropy distributions in relaxed muscle were relatively broad. At the peak of the distribution, the “lever” axis, linking Cys707 and Lys843 of the myosin heavy chain, was at 70–80° to the fiber axis, and the “hook” helix (Pro830–Lys843) was almost coplanar with the fiber and lever axes. The temperature and ionic strength of the relaxing solution had small but reproducible effects on the orientation of the RLC region. PMID:15041671