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Sample records for rg endocrinol metab

  1. Litiasis renal: estudio y manejo endocrinológico

    Directory of Open Access Journals (Sweden)

    V. Gilberto González, Dr.

    2013-09-01

    Full Text Available La litiasis renal es causa de importante morbilidad y costo económico, afectando hasta el 15% de la población. Además, la litiasis renal puede ser expresión también de enfermedades extrarrenales, entre las cuales destaca riesgo aumentado de osteoporosis. Estudios nacionales muestran que las causas de litiasis renal en Chile son similares a las comunicadas internacionalmente, destacando la hipercalciuria idiopática como el principal factor de riesgo. A pesar de los avances en técnicas urológicas de remoción de cálculo, éstas no modifican la historia natural de la litiasis renal. Así, en los pacientes sin tratamiento médico, la recurrencia es la regla más que la excepción. En esta revisión se actualiza el estudio y manejo endocrinológico de la litiasis renal, para el cual la evidencia muestra que éste es eficaz y seguro en la prevención de recurrencia de litiasis renal y control de enfermedades asociadas, complementando así el manejo urológico de esta importante enfermedad.

  2. Síndrome metabólico

    OpenAIRE

    Fériz Bonelo, Karen; Fundación Valle de Lili

    2007-01-01

    Síndrome metabólico/ ¿Cuál es la causa del Síndrome metabólico? / ¿Quién está en riesgo de sufrir síndrome metabólico?/ ¿Cómo se diagnostica el síndrome metabólico?/ ¿Cómo se trata el síndrome metabólico?/ ¿Cómo prevenir el síndrome metabólico?

  3. Metabolic programming of lipid profile and reproductive organs weight by leptin treatment on early life Programação metabólica do perfil lipídico e peso dos órgãos do sistema reprodutor pelo tratamento com leptina nos primeiros dias de vida

    Directory of Open Access Journals (Sweden)

    Durval Santos Marques

    2010-02-01

    leptina resultou em aumento significativo no peso corporal (Control= 411.8±16.31; Leptin= 481.8±11.29, p=0.005 e consumo alimentar (Control= 25.32±0.09; Leptin= 32.42±0.15, p=0.0001 e redução significativa nos níveis séricos de triglicerídeos (Control= 540.0±117.9; Leptin= 93.25±15.21, p=0.006, no peso do hipotálamo (Control= 0.234±0.016; Leptin= 0.154±0.015, p=0.007, hipófise (Control= 0.104±0.0120; Leptin= 0.033±0.012, p=0.003, testículo (Control= 3.75±0.055; Leptin= 3.19±0.10, p=0.002 e próstata (Control=1.641±0.1389; Leptin= 0.91±0.07, p=0.001. CONCLUSÃO: O tratamento com leptina nos primeiros dias de vida pode programar o peso dos órgãos do sistema reprodutor e o perfil lipídico da prole.

  4. Keterkaitan Rg-aljabar Dan Struktur Grup

    OpenAIRE

    Yudi, Irwan

    2014-01-01

    In this paper is discussed an algebraic structure which is called RG-algebra. The RG-algebra is a subclass of BCI-algebra and a sub subclass of K-algebra. Therefore, everyRG-algebra is a BCI-algebra, but conversly is not true. The RG-algebra andGroup Structure have a relation. Its relation is RG-algebra can be constructed by acommutativegroup and conversly commutativegroup can be constructed by a RG-algebra. The RG-algebra is a K-algebra with commutativegroupas generator group.

  5. Exotic RG flows from holography

    Energy Technology Data Exchange (ETDEWEB)

    Kiritsis, Elias [APC, Universite Paris 7, CNRS/IN2P3, CEA/IRFU, Obs. de Paris, Sorbonne Paris Cite (France); Crete Center for Theoretical Physics, Institute for Theoretical and Computational Physics, Department of Physics, University of Crete, Heraklion (Greece); Crete Center for Quantum Complexity and Nanotechnology, Department of Physics, University of Crete, Heraklion (Greece); Nitti, Francesco; Silva Pimenta, Leandro [APC, Universite Paris 7, CNRS/IN2P3, CEA/IRFU, Obs. de Paris, Sorbonne Paris Cite (France)

    2017-02-15

    Holographic RG flows are studied in an Einstein-dilaton theory with a general potential. The superpotential formalism is utilized in order to characterize and classify all solutions that are associated with asymptotically AdS space-times. Such solutions correspond to holographic RG flows and are characterized by their holographic β-functions. Novel solutions are found that have exotic properties from a RG point-of view. Some have β-functions that are defined patch-wise and lead to flows where the β-function changes sign without the flow stopping. Others describe flows that end in non-neighboring extrema in field space. Finally others describe regular flows between two minima of the potential and correspond holographically to flows driven by the VEV of an irrelevant operator in the UV CFT. (copyright 2017 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  6. Teledyne H1RG, H2RG, and H4RG Noise Generator

    Science.gov (United States)

    Rauscher, Bernard J.

    2015-01-01

    This paper describes the near-infrared detector system noise generator (NG) that we wrote for the James Webb Space Telescope (JWST) Near Infrared Spectrograph (NIRSpec). NG simulates many important noise components including; (1) white "read noise", (2) residual bias drifts, (3) pink 1/f noise, (4) alternating column noise, and (5) picture frame noise. By adjusting the input parameters, NG can simulate noise for Teledyne's H1RG, H2RG, and H4RG detectors with and without Teledyne's SIDECAR ASIC IR array controller. NG can be used as a starting point for simulating astronomical scenes by adding dark current, scattered light, and astronomical sources into the results from NG. NG is written in Python-3.4.

  7. Penrose limits and RG flows

    International Nuclear Information System (INIS)

    Gimon, Eric G.; Sonnenschein, Jacob; Pando Zayas, Leopoldo A.

    2002-01-01

    The Penrose-Gueven limit simplifies a given supergravity solution into a pp-wave background. Aiming at clarifying its relation to renormalization group flow we study the Penrose-Guven limit of supergravity backgrounds that are dual to non-conformal gauge theories. The resulting backgrounds fall in a class simple enough that the quantum particle is exactly solvable. We propose a map between the effective time-dependent quantum mechanical problem and the RG flow in the gauge theory. As a testing ground we consider explicitly two Penrose limits of the infrared fixed point of the Pilch-Warner solution. We analyze the corresponding gauge theory picture and write down the operators which are the duals of the low lying string states. We also address RG flows of a different nature by considering the Penrose-Gueven limit of a stack of N D p branes. We note that in the far IR (for p<3)the limit generically has negative mass-squared. This phenomenon signals, in the world sheet picture, the necessity to transform to another description. In this regard, we consider explicitly the cases of M2 from D2 and F1 from D1. (author)

  8. Diabetes mellitus. Urgencias metabólicas

    Directory of Open Access Journals (Sweden)

    Alfredo Darío Espinosa Brito

    2010-12-01

    Full Text Available En este artículo se realiza una revisión profunda sobra las características fundamentales de las complicaciones metabólicas que se pueden presentar en el paciente diabético. El propósito de dicha revisión es contribuir a una mejor identificación diagnóstica y establecer las pautas generales de tratamiento de las principales complicaciones metabólicas agudas de los diabéticos. Entre ellas, se consideran: la cetoacidosis diabética, el coma hiperglicémico hiperosmolar no cetósico, la acidosis láctica y la hipoglicemia. En ese sentido, se revisan los aspectos clínicos más importantes, los cambios metabólicos que ocurren en el organismo y las bases fundamentales para el tratamiento de estas complicaciones.An extensive review on the fundamental characteristics of the main metabolic complications that may occur in diabetic patients is presented in this article. Its purpose is to contribute to a better diagnosis and to establish general guidelines for the treatment of major acute metabolic complications in diabetic patients. Among those complications we have considered: diabetic ketoacidosis, hyperosmolar coma, nonketotic hyperglycemia, lactic acidosis and hypoglycemia. In that sense, an extensive review on the major clinical aspects, metabolic changes in the organism and fundamental basis for the treatment of such serious complications is presented.

  9. Strong disorder RG approach of random systems

    International Nuclear Information System (INIS)

    Igloi, Ferenc; Monthus, Cecile

    2005-01-01

    There is a large variety of quantum and classical systems in which the quenched disorder plays a dominant ro-circumflex le over quantum, thermal, or stochastic fluctuations: these systems display strong spatial heterogeneities, and many averaged observables are actually governed by rare regions. A unifying approach to treat the dynamical and/or static singularities of these systems has emerged recently, following the pioneering RG idea by Ma and Dasgupta and the detailed analysis by Fisher who showed that the Ma-Dasgupta RG rules yield asymptotic exact results if the broadness of the disorder grows indefinitely at large scales. Here we report these new developments by starting with an introduction of the main ingredients of the strong disorder RG method. We describe the basic properties of infinite disorder fixed points, which are realized at critical points, and of strong disorder fixed points, which control the singular behaviors in the Griffiths-phases. We then review in detail applications of the RG method to various disordered models, either (i) quantum models, such as random spin chains, ladders and higher dimensional spin systems, or (ii) classical models, such as diffusion in a random potential, equilibrium at low temperature and coarsening dynamics of classical random spin chains, trap models, delocalization transition of a random polymer from an interface, driven lattice gases and reaction diffusion models in the presence of quenched disorder. For several one-dimensional systems, the Ma-Dasgupta RG rules yields very detailed analytical results, whereas for other, mainly higher dimensional problems, the RG rules have to be implemented numerically. If available, the strong disorder RG results are compared with another, exact or numerical calculations

  10. Understanding the holographic principle via RG flow

    CERN Document Server

    Mukhopadhyay, Ayan

    2016-01-01

    This is a review of some recent works which demonstrate how the classical equations of gravity in AdS themselves hold the key to understanding their holographic origin in the form of a strongly coupled large $N$ QFT whose algebra of local operators can be generated by a few (single-trace) elements. I discuss how this can be realised by reformulating Einstein's equations in AdS in the form of a non-perturbative RG flow that further leads to a new approach towards constructing strongly interacting QFTs. In particular, the RG flow can self-determine the UV data that are otherwise obtained by solving classical gravity equations and demanding that the solutions do not have naked singularities. For a concrete demonstration, I focus on the hydrodynamic limit in which case this RG flow connects the AdS/CFT correspondence with the membrane paradigm, and also reproduces the known values of the dual QFT transport coefficients.

  11. Enfermedades Metabólicas del Hueso

    Directory of Open Access Journals (Sweden)

    Efraim Otero Ruiz

    1993-08-01

    Full Text Available

    Señoras, señores:

    Como se trata de una homologación de afectos y preferencias personales por los autores, colombianos y mexicanos, de esta obra; y como los dos hemos trajinado largo rato en esto de ser editores de revistas y libros, decidimos, el Dr. Jorge Maldonado y yo, echar a la suerte para ver quién hablaba en esta presentación formal del libro de Antonio Iglesias y colaboradores, titulado “Enfermedades metabólicas del hueso”.

    Yo gané la partida pero con la advertencia de que lo que aquí exprese, lo que se diga encomiásticamente del libro, de su autor principal y de su elenco de coautores, viene compenetradamente de nosotros dos y, para usar una expresión muy de la generación torera y española del 98, es como si hubiese sido escrito al alimón.

    Realmente, la aparición de esta publicación en sus dos volúmenes completos (el primero había aparecido en junio de este año marca un hito en la literatura médica escrita en nuestro idioma, ya que no se contaba con un texto castellano dedicado al hueso y sus enfermedades metabólicas. Ella no sólo denota el interés inveterado del autor principal, iniciado hace ya muchos años con la reumatología y el colágeno y preocupado después asiduamente por la osteoporosis y las enfermedades óseas, sino que le otorga además una especial capacidad de liderazgo ya que pudo, en un tiempo relativamente corto y sumergido en las responsabilidades directivas de un Instituto Nacional de Salud, lograr que una nómina ilustre, de 3 autores principales y 47 colaboradores, fuera construyendo, paso a paso, capítulo por capítulo, esta gran síntesis que destaca el esqueleto óseo como órgano de vital importancia metabólica y al tiempo asiento de muy disímiles patologías, muchas de ellas con causas aún no totalmente aclaradas.

    En efecto, durante muchos siglos se pensó en el esqueleto como estructura casi inerte que servía solamente de soporte o sostén a la

  12. Relative entropy and the RG flow

    Energy Technology Data Exchange (ETDEWEB)

    Casini, Horacio; Testé, Eduardo; Torroba, Gonzalo [Centro Atómico Bariloche and CONICET,S.C. de Bariloche, Río Negro, R8402AGP (Argentina)

    2017-03-16

    We consider the relative entropy between vacuum states of two different theories: a conformal field theory (CFT), and the CFT perturbed by a relevant operator. By restricting both states to the null Cauchy surface in the causal domain of a sphere, we make the relative entropy equal to the difference of entanglement entropies. As a result, this difference has the positivity and monotonicity properties of relative entropy. From this it follows a simple alternative proof of the c-theorem in d=2 space-time dimensions and, for d>2, the proof that the coefficient of the area term in the entanglement entropy decreases along the renormalization group (RG) flow between fixed points. We comment on the regimes of convergence of relative entropy, depending on the space-time dimensions and the conformal dimension Δ of the perturbation that triggers the RG flow.

  13. Prevalencia Síndrome metabólico en España

    OpenAIRE

    Pérez Tasigchana, Raúl Francisco

    2012-01-01

    Existen pocos estudios que han reportado datos nacionales de base poblacional para el Síndrome metabólico según las ultimas definiciones de la OMS. Este trabajo examina la prevalencia de Síndrome metabólico y síndrome metabólico premórbido en España.

  14. Exact RG flow equations and quantum gravity

    Science.gov (United States)

    de Alwis, S. P.

    2018-03-01

    We discuss the different forms of the functional RG equation and their relation to each other. In particular we suggest a generalized background field version that is close in spirit to the Polchinski equation as an alternative to the Wetterich equation to study Weinberg's asymptotic safety program for defining quantum gravity, and argue that the former is better suited for this purpose. Using the heat kernel expansion and proper time regularization we find evidence in support of this program in agreement with previous work.

  15. rg Specht: toetan niikaua, kui suudan / Jörg Specht ; interv. Elina Kononenko

    Index Scriptorium Estoniae

    Specht, Jörg

    2005-01-01

    Võru maakonna 2004. a. parimaks toetajaks valiti Jörg Specht, kes töötab Bad Segebergis ehitusettevõtjana ning on Võrumaale toonud suurel hulgal abipakke ja kingitusi. Mullu autasustati teda päästeameti hõberistiga. Vt. samas: Specht kinkis Kandlele oma auto

  16. RG cascades in hyperbolic quiver gauge theories

    International Nuclear Information System (INIS)

    Ahl Laamara, R.; Ait Ben Haddou, M.; Belhaj, A.; Drissi, L.B.; Saidi, E.H.

    2004-01-01

    In this paper, we provide a general classification of supersymmatric QFT4s into three basic sets: ordinary, affine and indefinite classes. The last class, which has not been enough explored in literature, is shown to share most of properties of ordinary and affine super-QFT4s. This includes, amongst others, its embedding in type II string on local Calabi-Yau threefolds. We give realizations of these supersymmetric QFT4s as D-brane world volume gauge theories. A special interest is devoted to hyperbolic subset for its peculiar features and for the role it plays in type IIB background with non-zero axion. We also study RG flows and duality cascades in case of hyperbolic quiver theories. Comments regarding the full indefinite sector are made

  17. Geometry of 6D RG flows

    International Nuclear Information System (INIS)

    Heckman, Jonathan J.; Morrison, David R.; Rudelius, Tom; Vafa, Cumrun

    2015-01-01

    We study renormalization group flows between six-dimensional superconformal field theories (SCFTs) using their geometric realizations as singular limits of F-theory compactified on elliptically fibered Calabi-Yau threefolds. There are two general types of flows: one corresponds to giving expectation values to scalars in the tensor multiplets (tensor branch flow) realized as resolving the base of the geometry. The other corresponds to giving expectation values to hypermultiplets (Higgs branch flow) realized as complex structure deformations of the geometry. To corroborate this physical picture we calculate the change in the anomaly polynomial for these theories, finding strong evidence for a flow from a UV fixed point to an IR fixed point. Moreover, we find evidence against non-trivial dualities for 6D SCFTs. In addition we find non-trivial RG flows for theories realizing small E 8 instantons on ALE spaces.

  18. Evaluation of Ginsenoside Rg1 as a Potential Antioxidant for ...

    African Journals Online (AJOL)

    Purpose: To determine whether Rg1 inhibits H2O2-induced injury in human umbilical vein endothelial cells (HUVECs), an injury often regarded as a key early event in the development of atherosclerosis. Methods: Cell viability of HUVECs treated with Rg1 and/or H2O2 was measured using 3-(4, 5- dimethylthiazol-2-yl)-2, ...

  19. Utilização da vitamina D3 e seus metabólitos na alimentação de frangos de corte sobre parâmetros imunológicos e morfometria intestinal

    Directory of Open Access Journals (Sweden)

    Ana Flávia Q. Garcia Guerra

    2014-05-01

    Full Text Available Os efeitos da suplementação da vitamina D3 e de seus diferentes metabólitos foram avaliados na resposta imune e na morfometria intestinal de frangos de corte. Foram utilizados 952 frangos de corte de um dia de idade, distribuídos em um delineamento inteiramente casualizado, com quatro tratamentos, sete repetições e 34 aves por unidade experimental. Os tratamentos foram constituídos por quatros diferentes fontes de vitamina D3: colecalciferol (D3, 25-hidroxicolecalciferol (25(OHD3, 1,25-dihidroxicolecalciferol (1,25(OH2D3 e 1α-hidroxicolecalciferol (1α(OHD3. As diferentes fontes foram incluídas na dieta, fornecendo 2000 e 1600 UI de vitamina D, nas fases pré-inicial, inicial e de crescimento, respectivamente. O peso relativo do intestino delgado diferiu entre os tratamentos aos 7, 21 e 42 dias e o peso relativo do fígado somente aos 42 dias de idade. Os demais órgãos e parâmetros imunológicos avaliados (peso dos órgãos linfóides, reação de hipersensibilidade cutânea basofílica, avaliação da atividade de macrófagos, dosagem de nitrito e perfil heterofilo: linfócito foram similares entre os animais alimentados com os diferentes metabólitos. Houve efeito (P0,05 dos tratamentos. Os parâmetros imunológicos não foram afetados pelos diferentes metabólitos de vitamina D. Os diferentes metabólitos de vitamina D afetaram positivamente a morfometria intestinal na altura de vilo na fase inicial, sendo os melhores resultados obtido pelos animais alimentados com 1,25(OH2D3, contudo os parâmetros imunológicos foram similares entre os metabólitos estudados.

  20. Síndrome Metabólico y Riesgo Cardiovascular.

    OpenAIRE

    Julio César Fernández-Travieso

    2016-01-01

    El síndrome metabólico (SM) se define como el conjunto de factores de riesgo cardiovascular constituido por obesidad de distribución central, dislipidemia caracterizada por elevación de las concentraciones de triglicéridos y disminución de las concentraciones de colesterol unido a lipoproteínas de alta densidad (HDL-C), anormalidades en el metabolismo de la glucosa e hipertensión arterial, estrechamente asociado a resistencia a la insulina. El SM se considera como predictor de morbilidad y mo...

  1. Síndrome metabólico en la mujer

    OpenAIRE

    Dora I. Molina de Salazar; Daniela Muñoz-Gómez

    2018-01-01

    Resumen: Introducción: La obesidad, especialmente la abdominal, se asocia con la resistencia al efecto de la insulina sobre la glucosa periférica y la utilización de ácidos grasos, hecho que puede conducir al desarrollo de síndrome metabólico y a la diabetes mellitus tipo 2. La resistencia a la insulina, la hiperinsulinemia y la hiperglucemia asociada, y el aumento de adipoquinas también puede llevar a la disfunción endotelial vascular, perfil anormal de lípidos, hipertensión e inflamación v...

  2. Ginsenoside Rg1 improves ischemic brain injury by balancing ...

    African Journals Online (AJOL)

    Ginsenoside Rg1 improves ischemic brain injury by balancing mitochondrial ... and autophagy-related proteins were determined by reat time-polymerase chain ... Treatment with autophagy inhibitors decreased the mitochondrial protective ...

  3. Ginsenoside Rg1 improves ischemic brain injury by balancing ...

    African Journals Online (AJOL)

    Tropical Journal of Pharmaceutical Research October 2017; 16 (10): 2469-2475 ... Keywords: Cerebral ischemia, Ginsenoside Rg1, Mitochondrial dysfunction, .... percentage of apoptotic death. .... d), which permit unrestricted use, distribution,.

  4. rg Haideri miljonid tekitavad kahtlusi / Marlen Rein

    Index Scriptorium Estoniae

    Rein, Marlen, 1990-

    2010-01-01

    Austria ajakiri Profil avaldas väljavõtteid Iraagi siseminiseeriumi toimikust, mis heidavad halba varju Austria parempopulistliku partei Austria Tulevikuallianss autoõnnetuses hukkunud liidrile Jörg Haiderile ja teistele prominentsetele poliitikutele

  5. Electrophysiolocal findings in Mohr-Tranebjærg syndrome

    Directory of Open Access Journals (Sweden)

    Regina Halfeld Furtado de Mendonça

    2015-04-01

    Full Text Available Mohr-Tranebjærg syndrome (MTS is an X-liked recessive rare syndrome also known as deafness-dystonia syndrome. The severity of the symptoms may vary, but they progress usually to severe deafness and dystonia and sometimes they are accompanied by cortical deterioration of vision and mental deterioration. The purpose of this paper is to illustrate a very interesting case of Mohr-Tranebjærg syndrome. A 24-year-old italian man with Mohr-Tranebjærg syndrome underwent full field electroretinography (ERG and visual evoked potentials (VEPs. Fundus examination showed apparently normal retina with pallor of the optic disc. Pattern reversal VEP and flash VEP responses were non-recordable. ERG showed amplitude reduction of the fotopic, scotopic and 30 Hz flicker responses revealing generalized retinal dysfunction with reduction of cone and rod responses. The progressive neurodegeneration in Mohr-Tranebjærg syndrome can be also associated with a retinal degeneration.

  6. Holography as a highly efficient RG flow I: Rephrasing gravity

    OpenAIRE

    Behr, Nicolas; Kuperstein, Stanislav; Mukhopadhyay, Ayan

    2015-01-01

    We investigate how the holographic correspondence can be reformulated as a generalisation of Wilsonian RG flow in a strongly interacting large $N$ quantum field theory. We firstly define a \\textit{highly efficient RG flow} as one in which the Ward identities related to local conservation of energy, momentum and charges preserve the same form at each scale -- to achieve this it is necessary to redefine the background metric and external sources at each scale as functionals of the effective sin...

  7. Biotransformation of ginsenosides F4 and Rg6 in zebrafish.

    Science.gov (United States)

    Shen, Wen-Wen; Zhang, Hai-Xia; Qiu, Shou-Bei; Wei, Ying-Jie; Zhu, Fen-Xia; Wang, Jing; Wang, Dan-Dan; Jia, Xiao-Bin; Tang, Dao-Quan; Chen, Bin

    2017-03-28

    Ginsenosides F 4 and Rg 6 (GF 4 and GRg 6 ), two main active components of steamed notoginseng or red ginseng, are dehydrated disaccharide saponins. In this work, biotransformation of ginsenosides F 4 and Rg 6 in zebrafish was investigated by qualitatively identifying their metabolites and then proposing their possible metabolic pathways. The prediction of possible metabolism of ginsenosides F 4 and Rg 6 using zebrafish model which can effectively simulate existing mammals model was early and quickly performed. Metabolites of ginsenosides F 4 and Rg 6 after exposing to zebrafish for 24 h were identified by Ultraperformance Liquid Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry. A total of 8 and 6 metabolites of ginsenosides F 4 and Rg 6 were identified in zebrafish, respectively. Of these, 7 and 5, including M1, M3-M5, M7-M9 and N1 (N5), N2, N4 (N9), N7-N8 were reported for the first time as far as we know. The mechanisms of their biotransformation involved were further deduced to be desugarization, glucuronidation, sulfation, dehydroxylation, loss of C-17 and/or C-23 residue pathways. It was concluded that loss of rhamnose at position C-6 and glucuronidation at position C-3 in zebrafish were considered as the main physiologic and metabolic processes of ginsenosides F 4 and ginsenosides Rg 6 , respectively.

  8. Síndrome metabólico en la mujer

    Directory of Open Access Journals (Sweden)

    Dora I. Molina de Salazar

    2018-01-01

    Full Text Available Resumen: Introducción: La obesidad, especialmente la abdominal, se asocia con la resistencia al efecto de la insulina sobre la glucosa periférica y la utilización de ácidos grasos, hecho que puede conducir al desarrollo de síndrome metabólico y a la diabetes mellitus tipo 2. La resistencia a la insulina, la hiperinsulinemia y la hiperglucemia asociada, y el aumento de adipoquinas también puede llevar a la disfunción endotelial vascular, perfil anormal de lípidos, hipertensión e inflamación vascular, todo lo cual promueve el desarrollo de enfermedad cardiovascular aterosclerótica; a esa asociación se le conoce como síndrome x, cuarteto de la muerte, síndrome de resistencia a la insulina.En el estudio NHANES III, el síndrome metabólico estaba presente en el 5% de los pacientes de peso normal, 22% de los que tenían sobrepeso, y el 60% de aquellos que eran obesos. El aumento del peso corporal es un factor de riesgo importante para el síndrome metabólico, además de la edad y la raza, otros factores asociados con un mayor riesgo de síndrome metabólico, el consumo de bebidas endulzadas con azúcar y medicamentos antipsicóticos. Objetivo: Determinar cómo influye el síndrome metabólico en la mujer en el aumento de riesgo cardiovascular. Método: Se realizó una revisión sistemática de las investigaciones y de las revisiones de tema que se han realizado en últimos años sobre el síndrome metabólico en la mujer, a partir de los hallazgos arrojados de bases de datos científicas. Uptodate, PubMed y SciELO. Conclusiones: La enfermedad cardiovascular es la principal causa de muerte en las mujeres y existen factores de riesgo específicos de la mujer para desarrollarla, entre los cuales están: edad temprana de menarquia, menopausia, síndrome premenstrual, síndrome de ovario poliquístico, uso de anticonceptivos orales, haber presentado trastorno hipertensivo del embarazo, parto pretérmino y otros factores de riesgo

  9. HM{sup +}–RG complexes (M = group 2 metal; RG = rare gas): Physical vs. chemical interactions

    Energy Technology Data Exchange (ETDEWEB)

    Harris, Joe P.; Dodson, Hannah; Wright, Timothy G., E-mail: Tim.Wright@nottingham.ac.uk [School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD (United Kingdom); Breckenridge, W. H. [Department of Chemistry, University of Utah, Salt Lake City, Utah 84112 (United States)

    2015-04-21

    Previous work on the HM{sup +}–He complexes (M = Be–Ra) has been extended to the cases of the heavier rare gas atoms, HM{sup +}–RG (RG = Ne–Rn). Optimized geometries and harmonic vibrational frequencies have been calculated using MP2 theory and quadruple-ζ quality basis sets. Dissociation energies for the loss of the rare gas atom have been calculated at these optimized geometries using coupled cluster with single and double excitations and perturbative triples, CCSD(T)theory, extrapolating interaction energies to the basis set limit. Comparisons are made between the present data and the previously obtained helium results, as well as to those of the bare HM{sup +} molecules; furthermore, comparisons are made to the related M{sup +}–RG and M{sup 2+}–RG complexes. Partial atomic charge analyses have also been undertaken, and these used to test a simple charge-induced dipole model. Molecular orbital diagrams are presented together with contour plots of the natural orbitals from the quadratic configuration with single and double excitations (QCISD) density. The conclusion is that the majority of these complexes are physically bound, with very little sharing of electron density; however, for M = Be, and to a lesser extent M = Mg, some evidence for chemical effects is seen in HM{sup +}–RG complexes involving RG atoms with the higher atomic numbers.

  10. Relaciones de la grasa visceral y de tronco con el sindrome metabólico

    OpenAIRE

    Fernández Vázquez, Rosalía Asunción

    2016-01-01

    La obesidad central es uno de los componentes que conforman el conjunto de factores del síndrome metabólico. La obesidad central se asocia a una gran incidencia de diabetes tipo 2, resistencia a la insulina, hipertensión arterial y particularmente a las enfermedades cardiovasculares. Las acciones metabólicas del tejido adiposo visceral son por sí solas un factor de riesgo independiente para una serie de alteraciones metabólicas. Objetivos: Los objetivos de la presente tesis han sido estudiar ...

  11. Programación metabólica fetal

    Directory of Open Access Journals (Sweden)

    R. Barrera Reyes

    2015-07-01

    Full Text Available En años recientes México es considerado como el país que tiene el primer lugar mundial de sobrepeso y/o obesidad en la población infantil. Organizaciones internacionales mencionan que el 33% de la población infantil de nuestro país padece sobrepeso y/o obesidad, los que representa un número muy grande de niños con riesgos de alteraciones a nivel metabólico, que al llegar a la edad adulta pueden tener lo que se ha denominado síndrome metabólico, el cual es una constelación de factores de riesgo cardiovascular, que incluye resistencia a la insulina, hipertensión, dislipidemia y obesidad central. En estudios realizados con animales se pudo probar que el feto puede adaptarse a la malnutrición, puede alterar su metabolismo, redistribuir el flujo sanguíneo para proteger los órganos más importantes como el cerebro, incluso, puede adaptarse a un crecimiento más lento para disminuir las exigencias de sustrato, pero esta adaptación lo puede llevar a cambios permanentes en la estructura y función de su organismo. La teoría Barker conocida también como efecto programador intra-útero, fue la primera en proponer el origen fetal de las enfermedades coronarias. El crecimiento y el desarrollo del feto están determinados por tres factores: el estado nutricional de la embarazada, la función placentaria y la capacidad del feto para utilizar los nutrientes.

  12. Functional perturbative RG and CFT data in the ϵ -expansion

    DEFF Research Database (Denmark)

    Codello, A.; Safari, M.; Vacca, G. P.

    2018-01-01

    We show how the use of standard perturbative RG in dimensional regularization allows for a renormalization group-based computation of both the spectrum and a family of coefficients of the operator product expansion (OPE) for a given universality class. The task is greatly simplified by a straight......We show how the use of standard perturbative RG in dimensional regularization allows for a renormalization group-based computation of both the spectrum and a family of coefficients of the operator product expansion (OPE) for a given universality class. The task is greatly simplified...... several results for the whole family of renormalizable multi-critical models ϕ2 n. Whenever comparison is possible our RG results explicitly match the ones recently derived in CFT frameworks....

  13. RG-inspired machine learning for lattice field theory

    Directory of Open Access Journals (Sweden)

    Foreman Sam

    2018-01-01

    Full Text Available Machine learning has been a fast growing field of research in several areas dealing with large datasets. We report recent attempts to use renormalization group (RG ideas in the context of machine learning. We examine coarse graining procedures for perceptron models designed to identify the digits of the MNIST data. We discuss the correspondence between principal components analysis (PCA and RG flows across the transition for worm configurations of the 2D Ising model. Preliminary results regarding the logarithmic divergence of the leading PCA eigenvalue were presented at the conference. More generally, we discuss the relationship between PCA and observables in Monte Carlo simulations and the possibility of reducing the number of learning parameters in supervised learning based on RG inspired hierarchical ansatzes.

  14. RG-inspired machine learning for lattice field theory

    Science.gov (United States)

    Foreman, Sam; Giedt, Joel; Meurice, Yannick; Unmuth-Yockey, Judah

    2018-03-01

    Machine learning has been a fast growing field of research in several areas dealing with large datasets. We report recent attempts to use renormalization group (RG) ideas in the context of machine learning. We examine coarse graining procedures for perceptron models designed to identify the digits of the MNIST data. We discuss the correspondence between principal components analysis (PCA) and RG flows across the transition for worm configurations of the 2D Ising model. Preliminary results regarding the logarithmic divergence of the leading PCA eigenvalue were presented at the conference. More generally, we discuss the relationship between PCA and observables in Monte Carlo simulations and the possibility of reducing the number of learning parameters in supervised learning based on RG inspired hierarchical ansatzes.

  15. Uus tootedisain = New product design / Monika Järg

    Index Scriptorium Estoniae

    Järg, Monika, 1975-

    2012-01-01

    Tutvustatakse: mööblisari Smart (Tarmo Luisk, 2011) ja Slice (Tarmo Luisk, 2008), rattahoidja Typo (Tarmo Luisk) ja Tulip Fan Fan (Margus Triibmann, 2011), iste Seat (Monika Järg), mööbliseeria Maast: tool CHO1 ja laud TBO1 (Tõnis Kalve, Aap Piho, 2011), padjad KO! (Kärt Ojavee, 2011), vaibakollektsioon Unlimited (Kaidi Ploomipuu, Monika Järg, 2011), vann Aquator CLAUDE 170 (2011), büroomööbli seeria Metod (Iseasi, 2012), rippvalgusti Aero (Tõnis Vellama, 2011), tool Must ja nagi Z (Toivo Raidmets, 2008-2012)

  16. Assimilação metabólica de nitrogênio em plântulas de seringueira cultivadas com nitrato ou amônio

    Directory of Open Access Journals (Sweden)

    Andrea Yu Kwan Villar Shan

    2012-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a assimilação metabólica de nitrogênio em plântulas de seringueira cultivadas na presença de nitrato ou de amônio, por meio da quantificação da atividade das enzimas redutase do nitrato (RN, glutamina sintetase (GS, glutamato sintase (GOGAT e glutamato desidrogenase (GDH. Os seguintes tratamentos foram avaliados: fontes de N - controle, sem aplicação de N; amônio a 8 mmol L-1; e nitrato a 8 mmol L-1 - e tempos de exposição - 0 e 27 dias -, em arranjo fatorial 3x2, no delineamento inteiramente casualizado, com quatro repetições. A forma amoniacal promoveu maior assimilação metabólica de N, o que resultou em maior acúmulo de aminoácidos. A assimilação, tanto da forma nítrica quanto da amoniacal, aconteceu principalmente via GS/GOGAT; parte do N nítrico é assimilado nas raízes, com pequena participação da GDH, e parte é translocada para outros órgãos. O N amoniacal é totalmente assimilado nas raízes, com significativa participação da GDH. Nas folhas, a GS participa tanto da assimilação primária como da reassimilação do amônio fotorrespirado, independentemente da fonte nitrogenada, enquanto a enzima GDH tem papel secundário, independentemente da fonte ou do tempo de exposição. A atividade da RN in vitro é detectada somente em raízes e caules.

  17. A functional RG equation for the c-function

    DEFF Research Database (Denmark)

    Codello, A.; D'Odorico, G.; Pagani, C.

    2014-01-01

    , local potential approximation and loop expansion. In each case we construct the relative approximate c-function and find it to be consistent with Zamolodchikov's c-theorem. Finally, we present a relation between the c-function and the (matter induced) beta function of Newton's constant, allowing us...... to use heat kernel techniques to compute the RG running of the c-function....

  18. [The bonding characteristic of titanium and RG experiment porcelain].

    Science.gov (United States)

    Ren, Wei-hong; Guo, Tian-wen; Tian, Jie-mo; Zhang, Yun-long

    2003-07-01

    To study the bonding characteristic of Titanium and RG experiment porcelain. 5 specimens with a size of 10 mm x 5 mm x 1.4 mm were cast from pure titanium. Then 1 mm of RG experiment opaque and body porcelain were fused on the surface of the titanium specimens. The interface of titanium and porcelain was analyzed with a scanning electron microscope with energy-despersive spectrometry; 6 metal specimens with the size of 25 mm x 3 mm x 0.5 mm were cast from Ni-Cr alloy and a uniform thickness of 1 mm of VMK 99 porcelain was veneered on the central area of 8 mm x 3 mm 18 metal specimens as the same size were cast from pure titanium. The uniform thickness of 1 mm of VITA TITANKERAMIK porcelain, of Noritake super porcelain Ti-22 and of RG experiment porcelain were veneered on every 6 specimens respectively in the central area of 8 mm x 3 mm. The specimens were subjected to a three-point bending test on a load-test machine with a span of 20 mm, then the failure loads were recorded and statistically analysised. The RG porcelain/titanium crown was fabricated by fusing RG opaque porcelain and body porcelain to cast titanium substrate crown. The SEM results show no porosity and crackle were found in the interface. The energy-dispersive spectrometry show that there are Si, Ti and O in the 1 micro m layer between porcelain and titanium, which suggesting titanium and experiment porcelain bonding well. The three point test showed the fracture force for the combinations of titanium/VITA TITANKERAMIK porcelain, titanium/Noritake super porcelain Ti-22 and titanium/RG experiment porcelain were (7.233 +/- 2.539) N, (5.533 +/- 1.199) N and (6.316 +/- 1.433) N respectively. There were not statistically significant differences among them (t test, P porcelain combination (12.733 +/- 3.297) N was significantly greater than those of the cast titanium/porcelain (t test, P > 0.05). The crown was translucent with no crack. RG porcelain is well compatible with titanium.

  19. Síndrome metabólico Metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Ana Liz Rodríguez Porto

    2002-12-01

    Full Text Available El síndrome metabólico fue reconocido hace más de 80 años en la literatura médica y ha recibido diversas denominaciones a través del tiempo. No se trata de una única enfermedad sino de una asociación de problemas de salud que pueden aparecer de forma simultánea o secuencial en un mismo individuo, causados por la combinación de factores genéticos y ambientales asociados al estilo de vida en los que la resistencia a la insulina se considera el componente patogénico fundamental. La presencia de síndrome metabólico se relaciona con un incremento significativo de riesgo de diabetes, enfermedad coronaria y enfermedad cerebrovascular, con disminución en la supervivencia, en particular, por el incremento unas 5 veces en la mortalidad cardiovascular. En la presente revisión se trataron aspectos relacionados con su patogenia, epidemiología y diagnóstico, se enfatizó además en la importancia de identificar y tratar oportunamente las comorbilidades presentes en estos pacientes como estrategia en la prevención de enfermedades cardiovasculares.The metabolic syndrome was recognized more than 80 years ago in medical literature and it has received since then several denominations. It is not a unique disease, but an association of health problems that may appear in a simultaneous or sequential way in the same individual caused by the combination of genetic and environmental factors associated with the life style, in which insulin resistance is considered as the main pathogenic component. The presence of metabolic syndrome is related to a significant increase of risk for diabetes, coronary disease and cerebrovascular disease, with a decrease of survival, particularly due to a 5-fold rise in cardiovascular mortality. Aspects connected with its pathogeny, epidemiology and diagnosis were dealt with in the present review. Emphasis was made on the importance of identifying and treating opportunely the comorbidities present in these patients as a

  20. 78 FR 8588 - Rg Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG...

    Science.gov (United States)

    2013-02-06

    ... Consulting, Crown Security, Eastern Automation, EDS(HP), Teksystems, URS Corporation, and B More Industrial... from B More Industrial Services LLC were employed on-site at the Sparrows Point, Maryland location of RG Steel Sparrows Point LLC. The Department has determined that these workers from B More Industrial...

  1. 77 FR 70478 - RG Steel Wheeling, LLC, Wheeling Office, A Division Of RG Steel, LLC, Including On-Site Leased...

    Science.gov (United States)

    2012-11-26

    ... Unlimited and Green Energy Initiatives LLC, Including Workers Whose Wages Were Reported Through Severstal..., Wheeling Office, a division of RG Steel, LLC, including on-site leased workers from Pro Unlimited and Green Energy Initiatives, LLC, Wheeling, West Virginia (TA-W-81,880) and Mountain State Carbon, LLC, including...

  2. Neuroprotective effects of ginsenoside Rg1 against oxygen–glucose deprivation in cultured hippocampal neurons

    OpenAIRE

    He, Qing; Sun, Jianguo; Wang, Qin; Wang, Wei; He, Bin

    2014-01-01

    Background: Ginsenoside Rg1 (Rg1) is believed to be one of the main active principles in ginseng, a traditional Chinese medicine extensively used to enhance stamina and deal with fatigue as well as physical stress. It has been reported that Rg1 performs multiple biological activities, including neuroprotective activity. In this study, we investigated the efficacy of ginsenoside Rg1 on ischemia–reperfusion injury in cultured hippocampal cells and also probed its possible mechanisms. Methods...

  3. Síndrome metabólico y enfermedad renal

    Directory of Open Access Journals (Sweden)

    P. Alfredo Molina, Dr.

    2010-07-01

    Full Text Available Estudios epidemiológicos han señalado que el Síndrome Metabólico (SM es un factor de riesgo independiente, que incidiría en el desarrollo de daño renal crónico, existiendo además una relación gradual entre el número de componentes del SM y la prevalencia de enfermedad renal crónica (ERC. El SM corresponde a una agrupación de factores de riesgo: Obesidad abdominal, hipertrigliceridemia, colesterol HDL bajo, elevación de la presión arterial, elevación de la glicemia en ayunas, que se asocian a un aumento del riesgo de desarrollo de diabetes mellitus y enfermedades cardiovasculares. Debido a limitaciones metodológicas de los estudios actuales, no ha sido posible demostrar si el SM es causa o efecto de ERC. Se describen los mecanismos fisiopatológicos del SM y de su asociación a ERC. La American Heart Association recomienda un manejo agresivo y enfocado en los múltiples factores de riesgo del SM; sin embargo, aún no existen estudios que hayan evaluado si este tipo de intervenciones en pacientes con SM puede reducir la incidencia o la progresión de ERC. Se requieren estudios prospectivos e intervencionales que permitan medir de manera exacta el impacto del tratamiento del SM y la obesidad en el riesgo de ERC.

  4. Holographic RG flows on curved manifolds and quantum phase transitions

    Science.gov (United States)

    Ghosh, J. K.; Kiritsis, E.; Nitti, F.; Witkowski, L. T.

    2018-05-01

    Holographic RG flows dual to QFTs on maximally symmetric curved manifolds (dS d , AdS d , and S d ) are considered in the framework of Einstein-dilaton gravity in d + 1 dimensions. A general dilaton potential is used and the flows are driven by a scalar relevant operator. The general properties of such flows are analyzed and the UV and IR asymptotics computed. New RG flows can appear at finite curvature which do not have a zero curvature counterpart. The so-called `bouncing' flows, where the β-function has a branch cut at which it changes sign, are found to persist at finite curvature. Novel quantum first-order phase transitions are found, triggered by a variation in the d-dimensional curvature in theories allowing multiple ground states.

  5. The nanosecond generator RG-1 with near-rectangular pulse

    International Nuclear Information System (INIS)

    Bulan, V.V.; Grabovskij, E.V.; Gribov, A.N.; Luzhnov, V.G.

    1996-01-01

    The 300 kV, 17 Ohm generator RG-1, which can deliver near-rectangular pulses with a pulse duration of 80 ns FWHM, is described. The polarity of the output pulse can be changed by a simple switch. The fast capacities of the Marx generator are used instead of the pulse forming line. Multi-spark gas switches were developed to decrease the inductance of the discharged circuit. The generator is supplied by a built-in high voltage source and its operation is controlled by a minicomputer. It is used the power supply-line 220 V. The RG-1 can be used in different modes of operation: gas discharge, particle beam formation, etc. (author). 4 figs., 3 refs

  6. Radiation and drug response of the rat glioma RG2

    International Nuclear Information System (INIS)

    Weizsaecker, M.; Nagamune, A.; Winkelstroeter, R.; Vieten, H.; Wechsler, W.

    1982-01-01

    A clonogenic cell assay was developed for the chemically induced rat glioma RG2 that allows in vivo, in vitro, and in vivo to in vitro studies of cell survival after experimental therapy. RG2 monolayer cells were resistant to BCNU up to high concentrations. The x-radiation survival curves were characterized by a D 0 of 2.4 gray and n = 2.2 for monolayer cells, a D 0 of 3.5 gray and n = 1.3 for cells irradiated as brain tumors in air-breathing rats, and a D 0 of 5.9 gray and n = 1.2 for cells irradiated as brain tumors in nitrogen-asphyxiated rats. There was no evidence of a radiobiologically hypoxic fraction of cells in the brain tumors, but their radiosensitivity was definitely smaller than that of monolayer cells. (author)

  7. The nanosecond generator RG-1 with near-rectangular pulse

    Energy Technology Data Exchange (ETDEWEB)

    Bulan, V V; Grabovskij, E V; Gribov, A N; Luzhnov, V G [TRINITI, Troitsk (Russian Federation)

    1997-12-31

    The 300 kV, 17 Ohm generator RG-1, which can deliver near-rectangular pulses with a pulse duration of 80 ns FWHM, is described. The polarity of the output pulse can be changed by a simple switch. The fast capacities of the Marx generator are used instead of the pulse forming line. Multi-spark gas switches were developed to decrease the inductance of the discharged circuit. The generator is supplied by a built-in high voltage source and its operation is controlled by a minicomputer. It is used the power supply-line 220 V. The RG-1 can be used in different modes of operation: gas discharge, particle beam formation, etc. (author). 4 figs., 3 refs.

  8. Constraints on perturbative RG flows in six dimensions

    Energy Technology Data Exchange (ETDEWEB)

    Stergiou, Andreas [Department of Physics, Yale University,217 Prospect St, New Haven, CT 06520 (United States); Stone, David [INFN, Sezione di Roma,Piazzale A. Moro 2, I-00185 Roma (Italy); Vitale, Lorenzo G. [Institut de Théorie des Phènoménes Physiques, EPFL,Route Cantonale, CH-1015 Lausanne (Switzerland)

    2016-08-01

    When conformal field theories (CFTs) are perturbed by marginally relevant deformations, renormalization group (RG) flows ensue that can be studied with perturbative methods, at least as long as they remain close to the original CFT. In this work we study such RG flows in the vicinity of six-dimensional unitary CFTs. Neglecting effects of scalar operators of dimension two and four, we use Weyl consistency conditions to prove the a-theorem in perturbation theory, and establish that scale implies conformal invariance. We identify a quantity that monotonically decreases in the flow to the infrared due to unitarity, showing that it does not agree with the one studied recently in the literature on the six-dimensional ϕ{sup 3} theory.

  9. Síndrome metabólico en adolescentes con sobrepeso y obesidad

    Directory of Open Access Journals (Sweden)

    Jaime Pajuelo

    2007-06-01

    Full Text Available Objetivo: Conocer y cuantificar la presencia de síndrome metabólico en una población adolescente que presenta sobrepeso y obesidad. Diseño: Estudio clínico. Lugar: Servicio de Endocrinología. Hospital Nacional Dos de Mayo. Participantes: Adolescentes. Intervenciones: Se estudió una muestra de 195 adolescentes comprendidos entre los 10 a 19 años. El criterio de inclusión fue que presentasen sobrepeso u obesidad, de acuerdo al índice de masa corporal (IMC. Para ello, se hizo uso de la población de referencia de Must y col, con los siguientes criterios diagnósticos: de 85 a 95 percentil, sobrepeso; y mayor del 95 percentil, obesos. El peso, la talla y la circunferencia de la cintura (CC fueron tomados siguiendo las recomendaciones internacionales. El síndrome metabólico fue definido mediante la recomendación de Cook (triglicéridos > 110, colesterol HDL 110 mg/dL y CC > 90 percentil, la presencia de tres o más de estos criterios indicó síndrome metabólico. Principales medidas de resultados: Síndrome metabólico, sobrepeso, obesidad. Resultados: En 8,8% de la muestra estudiada existió la presencia de síndrome metabólico. Los obesos presentaron mayor prevalencia de síndrome metabólico que los con sobrepeso (22,9 y 3%, respectivamente; el género masculino (24,1% más que el femenino (2,9%. Los de 15 años y más tuvieron un 9,7%, con relación a los menores, 7,4%. Conforme se incrementaba la CC, la presencia de síndrome metabólico fue mayor. Conclusiones: El síndrome metabólico se encuentra presente en la población adolescente, si bien con prevalencias bajas, pero que están íntimamente relacionadas al estado nutricional (sobrepeso y obesidad. La tendencia de estas alteraciones nutricionales es a incrementarse y como consecuencia mucho más adolescentes presentaran el síndrome metabólico, lo que lleva implícito un riesgo para su salud.

  10. Alteradores endocrinos y desórdenes metabólicos y reproductivos: Perspectivas futuras

    Directory of Open Access Journals (Sweden)

    Jaime Mendiola Olivares

    2014-06-01

    Full Text Available Es evidente la importancia que está adquiriendo la asociación entre exposiciones medioambientales (principalmente compuestos alteradores endocrinos y la afectación de la salud humana. Estos compuestos incluyen una gran variedad de sustancias químicas, tanto de uso hortofrutícola (compuestos organoclorados y organofosforados, fungicidas, etc., como industrial o comercial (bisfenol A, ftalatos, compuestos perfluorados, etc.. Actualmente, las principales líneas de investigación incluyen los desórdenes en el neurodesarrollo o el cáncer, junto con alteraciones o enfermedades hormonales, metabólicas o reproductivas. La incidencia de desórdenes metabólicos como la obesidad, el síndrome metabólico o la diabetes, y los problemas reproductivos o de infertilidad están incrementándose en poblaciones humanas. No obstante, los factores de riesgo establecidos no pueden explicar completamente las tendencias observadas para estos desórdenes. En general, sería extremadamente recomendable aumentar el número de estudios epidemiológicos en humanos y mecanísticos en modelos preclínicos o celulares que exploren las asociaciones entre exposición a alteradores endocrinos y desórdenes o enfermedades metabólicas como la obesidad, el síndrome metabólico, la diabetes o la infertilidad, incluyendo aspectos epigenéticos.

  11. Per Nørgårds “nordiske" periode

    DEFF Research Database (Denmark)

    Nielsen, Rie B.; Bonde, Anders

    1997-01-01

    s. 491-653 + bilag  Per Nørgård (f. 1932) er en af de nulevende danske komponister, hvis musik har givet anledning til flest skriverier. Det være sig i bøger, aviser, tidsskrifter m.m. Samtidigt foreligger fra komponisten selv et væld af artikler og manuskripter, omhandlende musikteori, analyser......, kulturkritik m.m. I en lang række af disse skrifter fra især 1980'erne og 1990'erne dukker ordet "interferens" til stadighed op i såvel Per Nørgårds egne som andres udtalelser og kommentarer, knyttet til hans kompositoriske virke. Interferensfænomenet har med tiden vist sig at have en helt central placering i...... forbindelse med hans musik, dog uden at der på noget tidspunkt er opstillet fyldestgørende definitioner af begrebet. Samtidig har Per Nørgård flere gange fremhævet, hvorledes interferensen synes at stå som en overordnet fællesnævner for hele hans produktion trods store stilistiske forskelle. Som følge deraf...

  12. Boomerang RG flows in M-theory with intermediate scaling

    Science.gov (United States)

    Donos, Aristomenis; Gauntlett, Jerome P.; Rosen, Christopher; Sosa-Rodriguez, Omar

    2017-07-01

    We construct novel RG flows of D=11 supergravity that asymptotically approach AdS 4 × S 7 in the UV with deformations that break spatial translations in the dual field theory. In the IR the solutions return to exactly the same AdS 4 × S 7 vacuum, with a renormalisation of relative length scales, and hence we refer to the flows as `boomerang RG flows'. For sufficiently large deformations, on the way to the IR the solutions also approach two distinct intermediate scaling regimes, each with hyperscaling violation. The first regime is Lorentz invariant with dynamical exponent z = 1 while the second has z = 5/2. Neither ofthe two intermediatescaling regimesare associatedwith exact hyperscaling violation solutions of D = 11 supergravity. The RG flow solutions are constructed using the four dimensional N = 2 STU gauged supergravity theory with vanishing gauge fields, but non-vanishing scalar and pseudoscalar fields. In the ABJM dual field theory the flows are driven by spatially modulated deformation parameters for scalar and fermion bilinear operators.

  13. Supersymmetric RG flows and Janus from type II orbifold compactification

    Energy Technology Data Exchange (ETDEWEB)

    Karndumri, Parinya; Upathambhakul, Khem [Chulalongkorn University, String Theory and Supergravity Group, Department of Physics, Faculty of Science, Bangkok (Thailand)

    2017-07-15

    We study holographic RG flow solutions within four-dimensional N = 4 gauged supergravity obtained from type IIA and IIB string theories compactified on T{sup 6}/Z{sub 2} x Z{sub 2} orbifold with gauge, geometric and non-geometric fluxes. In type IIB non-geometric compactifications, the resulting gauged supergravity has ISO(3) x ISO(3) gauge group and admits an N = 4 AdS{sub 4} vacuum dual to an N = 4 superconformal field theory (SCFT) in three dimensions. We study various supersymmetric RG flows from this N = 4 SCFT to N = 4 and N = 1 non-conformal field theories in the IR. The flows preserving N = 4 supersymmetry are driven by relevant operators of dimensions Δ = 1, 2 or alternatively by one of these relevant operators, dual to the dilaton, and irrelevant operators of dimensions Δ = 4 while the N = 1 flows in addition involve marginal deformations. Most of the flows can be obtained analytically. We also give examples of supersymmetric Janus solutions preserving N = 4 and N = 1 supersymmetries. These solutions should describe two-dimensional conformal defects within the dual N = 4 SCFT. Geometric compactifications of type IIA theory give rise to N = 4 gauged supergravity with ISO(3) x U(1){sup 6} gauge group. In this case, the resulting gauged supergravity admits an N = 1 AdS{sub 4} vacuum. We also numerically study possible N = 1 RG flows to non-conformal field theories in this case. (orig.)

  14. Ejercicio, Obesidad y Sindrome Metabólico

    Directory of Open Access Journals (Sweden)

    A. Carlos Jorquera, Nut. Msc

    2012-05-01

    Full Text Available Los bajos niveles de actividad física y/o ejercicio, junto a los malos hábitos alimentarios presentes a nivel nacional e internacional, han traído consigo una serie de complicaciones y/o morbilidades que han provocado un importante aumento de las tasas de mortalidad por enfermedades adquiridas, muchas de ellas ligadas a esta malnutrición por exceso y sedentarismo, descritos en encuestas nacionales e internacionales. La falta de movimiento es extrema en la población chilena y mundial, cada día la industria desarrolla elementos que buscan disminuir la ejecución de ejercicio físico, lo que se refleja de cierta manera en las encuestas ejecutadas, donde las tasas de inactividad física son elevadísimas, tanto en adultos como en niños, ya sean estos hombres o mujeres. Esto, sumado a los elevados niveles de obesidad, hace que enfoquemos nuestra atención al desarrollo de un tratamiento que combata estos dos factores de riesgo de manera paralela, pues en la ecuación de Balance Energético un 50% de ella corresponde a la ingesta y un 50% al gasto de energía. Los elevados niveles de sobrepeso y obesidad de nuestra realidad chilena (60% de la población, son la evidencia de un balance energético positivo provocado por una mayor ingesta de alimentos y un menor gasto en actividad física lo que ha llevado a que aumente progresivamente el Síndrome Metabólico y las ECNT en nuestra población.

  15. Efeito do sulfato de vanadil sobre o comprometimento metabólico muscular induzido pela imobilização de membro posterior de ratos Efecto del sulfato de vanadil sobre comprometimiento metabólico muscular inducido por la inmovilización del miembro posterior en ratones Effect of the vanadyl sulphate on the muscular metabolic compromising induced by immobilization of posterior limb of rats

    Directory of Open Access Journals (Sweden)

    Gabriel Borges Delfino

    2006-12-01

    Full Text Available A proposta deste trabalho foi avaliar o efeito do sulfato de vanadil (SV no perfil metabólico muscular de membro posterior imobilizado de ratos. Ratos Wistar foram divididos nos grupos (n = 6: controle (C, imobilizado em posição neutra do tornozelo (I, tratado com sulfato de vanadil (SV, 0,25mM, VO e imobilizado tratado com SV (I + SV durante sete dias. Após o período experimental, foram avaliadas as reservas de glicogênio (RG dos músculos sóleo (S, gastrocnêmio branco (GB e vermelho (GV, tibial anterior (TA e extensor longo dos dedos (ELD, além do peso do S e ELD. A análise estatística foi realizada pela ANOVA seguida pelo teste de Tukey (p La propuesta de este trabajo ha sido la de evaluar el efecto del sulfato de vanadil (SV en el perfil metabólico muscular de miembro posterior inmovilizado de ratones. Ratones Wistar fueron divididos en grupos (n = 6: control (C, inmovilizado en posición neutra de tobillo (I, tratado con sulfato de vanadil (SV, 0,25mM, VO e inmovilizado tratado con SV (I + SV durante 7 días. Después del periodo experimental, fueron evaluadas las reservas de glicógeno (RG de los músculos soleo (S, gastrocnemio blanco (GB y colorado (GV, tibial anterior (TA y extensor largo de los dedos (ELD, además del peso de S y ELD. El análisis estadístico fue realizado por ANOVA seguido del test de Tukey (p The purpose of this study was to evaluate the metabolic performance of immobilized skeletal muscle in rats treated with vanadyl sulphate. Male Wistar rats were divided in groups (n = 6: control (C, immobilized (I, treated with vanadyl sulphate (VS, 0,25 mM and immobilized treated with vanadyl sulphate (I + VS during seven days. The concentration of vanadyl sulphate diluted in water was 0,25 mM. After experimental stage, the glycogen content (GC was evaluated in soleus (S, white gastrocnemius (WG, red gastrocnemius (RG, tibialis anterior (TA and extensor digitorum longus (EDL muscles, besides S and EDL weight. The

  16. Nutrición y síndrome metabólico.

    OpenAIRE

    Pilar Matía Martín; Edurne Lecumberri Pascual; Alfonso L. Calle Pascual

    2007-01-01

    Existe suficiente evidencia sobre la asociación de alteraciones en el metabolismo de la glucosa, las lipoproteínas, la acción de la insulina, la hipertensión y la obesidad de distribución central. Esta asociación se denomina Síndrome Metabólico. A pesar de que ha sido cuestionada su existencia por la ADA y la EASD, es una herramienta útil que permite identificar a las personas que tienen un alto riesgo de desarrollar enfermedad cardiovascular. El síndrome metabólico y sus ...

  17. Evaluación metabólica y nutricional en litiasis renal

    OpenAIRE

    Rodrigo Orozco, B.; Carolina Camaggi, M.

    2010-01-01

    Los pacientes con litiasis renal requieren de investigaciones para identificar las condiciones médicas subyacentes y otras anomalías metabólicas predisponentes. Los resultados de estas investigaciones se utilizan para guiar el tratamiento preventivo. La profundidad del estudio necesario depende de varios factores, incluyendo la edad, la historia clínica de la persona y el número y la frecuencia de los cálculos. Una variedad de factores dietéticos y metabólicos pueden contribuir o causar la fo...

  18. Síndrome metabólico durante el embarazo: Complicaciones materno-fetales

    OpenAIRE

    Yépez, Mayel Cristina; Zeppenfel, María Eugenia; Colón, José Antonio; Zimmer, Eveline

    2011-01-01

    Objetivo: Determinar la asociación entre el síndrome metabólico y las complicaciones maternas, fetales y neonatales, en un grupo de embarazadas entre agosto 2008 y septiembre 2009. Ambiente: Servicio Prenatal de la Maternidad “Concepción Palacios”. Métodos: Estudio prospectivo, longitudinal, comparativo, con una muestra de 130 embarazadas, 38 cumplieron los criterios de la Federación Internacional de Diabetes para síndrome metabólico y 92 fueron el grupo control. Resultados: El promedio de ed...

  19. Holographic RG flows from Quasi-Topological Gravity

    International Nuclear Information System (INIS)

    Camara da Silva, U.; Sotkov, G.M.

    2013-01-01

    We investigate the holographic Renormalization Group (RG) flows and the critical phenomena that take place in the QFT's dual to the d-dimensional cubic Quasi-Topological Gravity coupled to scalar matter. The knowledge of the corresponding flat Domain Walls (DW's) solutions allows us to derive the explicit form of the QFT's β-functions, as well as of the trace anomalies a(l) and c(l), in terms of the matter superpotential. As a consequence we are able to determine the complete set of CFT data characterizing the universality classes of the UV and IR critical points and to follow the particular RG evolution of this data. We further analyse the dependence of the critical properties of such dual QFT's on the values of the Lovelock couplings and on the shape of the superpotential. For odd values of d, the explicit form of the “a and c-central charges” as functions of the running coupling constant, enable us to establish the conditions under which the a and c-Theorems for their decreasing are valid. The restrictions imposed on the massless holographic RG flows by the requirements of the positivity of the energy fluxes are derived. The particular case of quartic Higgs-like superpotential is studied in detail. It provides an example of unitary dual QFT's having few c≠a-critical points representing second or infinite order phase transitions. Depending on the range of the values of the coupling constant they exhibit massive and massless phases, described by a chain of distinct DW's solutions sharing common boundaries

  20. Determination of Gravitational Counterterms Near Four Dimensions from RG Equations

    OpenAIRE

    Hamada, Ken-ji

    2014-01-01

    The finiteness condition of renormalization gives a restriction on the form of the gravitational action. By reconsidering the Hathrell's RG equations for massless QED in curved space, we determine the gravitational counterterms and the conformal anomalies as well near four dimensions. As conjectured for conformal couplings in 1970s, we show that at all orders of the perturbation they can be combined into two forms only: the square of the Weyl tensor in $D$ dimensions and $E_D=G_4 +(D-4)\\chi(D...

  1. R.G. Collingwood, Analytical Philosophy And Logical Positivism

    Directory of Open Access Journals (Sweden)

    James Connelly

    2009-10-01

    Full Text Available R.G. Collingwood is not normally associated with analytic philosophy, neither negatively nor positively. He neither regarded himself, nor was regarded by his contemporaries and their successors, as an analytical philosopher. However, the story is more interestingly complex than this, both because Collingwood is one of the few pre-analytics in the UK who continues to be of interest to current analytical philosophers, especially in relation to the philosophy of art and history and his conception of metaphysics, and because he mounted a critique of analytical philosophy in the years of its emergence.

  2. Ginsenoside Rg3 improves cardiac mitochondrial population quality: Mimetic exercise training

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Mengwei [Key Laboratory of State General Administration of Sport, Shanghai Research Institute of Sports Science, Shanghai 200031 (China); Huang, Chenglin [Shanghai Key Laboratory of Vascular Biology, Department of Hypertension and Pharmacology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200025 (China); Wang, Cheng; Zheng, Jianheng; Zhang, Peng; Xu, Yangshu [Key Laboratory of State General Administration of Sport, Shanghai Research Institute of Sports Science, Shanghai 200031 (China); Chen, Hong, E-mail: hchen100@hotmail.com [Shanghai Key Laboratory of Vascular Biology, Department of Hypertension and Pharmacology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200025 (China); Shen, Weili, E-mail: weili_shen@hotmail.com [Shanghai Key Laboratory of Vascular Biology, Department of Hypertension and Pharmacology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200025 (China)

    2013-11-08

    Highlights: •Rg3 is an ergogenic aid. •Rg3 improves mitochondrial antioxidant capacity. •Rg3 regulates mitochondria dynamic remodeling. •Rg3 alone matches some the benefits of aerobic exercise. -- Abstract: Emerging evidence indicates exercise training could mediate mitochondrial quality control through the improvement of mitochondrial dynamics. Ginsenoside Rg3 (Rg3), one of the active ingredients in Panax ginseng, is well known in herbal medicine as a tonic and restorative agent. However, the molecular mechanism underlying the beneficial effects of Rg3 has been elusive. In the present study, we compared the effects of Rg3 administration with aerobic exercise on mitochondrial adaptation in cardiac muscle tissue of Sprague–Dawley (SD) rats. Three groups of SD rats were studied: (1) sedentary control, (2) Rg3-treated and (3) aerobic exercise trained. Both aerobic exercise training and Rg3 supplementation enhanced peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) and nuclear factor-E2-related factor 2 (Nrf2) protein levels in cardiac muscle. The activation of PGC-1α led to increased mRNA levels of mitochondrial transcription factor A (Tfam) and nuclear related factor 1(Nrf1), these changes were accompanied by increases in mitochondrial DNA copy number and complex protein levels, while activation of Nrf2 increased levels of phase II detoxifying enzymes, including nicotinamide adenine dinucleotide phosphate:quinone oxidoreductase 1(NQO1), superoxide dismutase (MnSOD) and catalase. Aerobic exercise also enhanced mitochondrial autophagy pathway activity, including increased conversion of LC3-I to LC3-II and greater expression of beclin1 and autophagy-related protein 7 (ATG7), these effects of aerobic exercise are comparable to that of Rg3. These results demonstrate that Rg3 mimics improved cardiac adaptations to exercise by regulating mitochondria dynamic remodeling and enhancing the quantity and quality of mitochondria.

  3. Ginsenoside Rg3 improves cardiac mitochondrial population quality: Mimetic exercise training

    International Nuclear Information System (INIS)

    Sun, Mengwei; Huang, Chenglin; Wang, Cheng; Zheng, Jianheng; Zhang, Peng; Xu, Yangshu; Chen, Hong; Shen, Weili

    2013-01-01

    Highlights: •Rg3 is an ergogenic aid. •Rg3 improves mitochondrial antioxidant capacity. •Rg3 regulates mitochondria dynamic remodeling. •Rg3 alone matches some the benefits of aerobic exercise. -- Abstract: Emerging evidence indicates exercise training could mediate mitochondrial quality control through the improvement of mitochondrial dynamics. Ginsenoside Rg3 (Rg3), one of the active ingredients in Panax ginseng, is well known in herbal medicine as a tonic and restorative agent. However, the molecular mechanism underlying the beneficial effects of Rg3 has been elusive. In the present study, we compared the effects of Rg3 administration with aerobic exercise on mitochondrial adaptation in cardiac muscle tissue of Sprague–Dawley (SD) rats. Three groups of SD rats were studied: (1) sedentary control, (2) Rg3-treated and (3) aerobic exercise trained. Both aerobic exercise training and Rg3 supplementation enhanced peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) and nuclear factor-E2-related factor 2 (Nrf2) protein levels in cardiac muscle. The activation of PGC-1α led to increased mRNA levels of mitochondrial transcription factor A (Tfam) and nuclear related factor 1(Nrf1), these changes were accompanied by increases in mitochondrial DNA copy number and complex protein levels, while activation of Nrf2 increased levels of phase II detoxifying enzymes, including nicotinamide adenine dinucleotide phosphate:quinone oxidoreductase 1(NQO1), superoxide dismutase (MnSOD) and catalase. Aerobic exercise also enhanced mitochondrial autophagy pathway activity, including increased conversion of LC3-I to LC3-II and greater expression of beclin1 and autophagy-related protein 7 (ATG7), these effects of aerobic exercise are comparable to that of Rg3. These results demonstrate that Rg3 mimics improved cardiac adaptations to exercise by regulating mitochondria dynamic remodeling and enhancing the quantity and quality of mitochondria

  4. The effect of recycled Natural Rubber Glove (rRG) Plasticizers to Deflection and Flexural Strength Properties of PP/MMt/rRG Smart Composites and Its Inflammability

    Science.gov (United States)

    Suharty, N. S.; Ismail, H.; Diharjo, K.; Handayani, D. S.; Saputri, L. N. M. Z.; Ariesta, N.

    2018-03-01

    Had been synthesized PP/rRG/MMt+ZB smart material composite in solution reactive processes with various rRG concentration. The addition of rRG plasticizers will improve the deflection properties and increase the filler capacity MMt loading to reach the optimum concentration. The addition of 3% rRG is capable of loading filler capacity MMt to 23% as the optimum condition. At the optimum conditions it can increase the deflection (Defl) and flexural strength (FS) up to 16% and 15% respectively compared to that of the composites without rRG. The rRG plasticizer serves as a bio-compatibilizer that can reduce surface tension of the mixture and leads to decrease the Defl., follow by the increase of loading filler capacity and well interaction finally can increase the FS properties. The increase of loading filler MMt up to 23% can also improve the inflammability of the composites. Time to Ignition (TTI) increase by 5% and Burning Rate (BR) decrease by 4.5% compared to that of the composites which is containing MMt 20% without rRG.

  5. Entanglement and RG in the O(N) vector model

    International Nuclear Information System (INIS)

    Akers, Chris; Ben-Ami, Omer; Rosenhaus, Vladimir; Smolkin, Michael; Yankielowicz, Shimon

    2016-01-01

    We consider the large N interacting vector O(N) model on a sphere in 4−ϵ Euclidean dimensions. The Gaussian theory in the UV is taken to be either conformally or non-conformally coupled. The endpoint of the RG flow corresponds to a conformally coupled scalar field at the Wilson-Fisher fixed point. We take a spherical entangling surface in de Sitter space and compute the entanglement entropy everywhere along the RG trajectory. In 4 dimensions, a free non-conformal scalar has a universal area term scaling with the logarithm of the UV cutoff. In 4−ϵ dimensions, such a term scales as 1/ϵ. For a non-conformal scalar, a 1/ϵ term is present both at the UV fixed point, and its vicinity. For flow between two conformal fixed points, 1/ϵ terms are absent everywhere. Finally, we make contact with replica trick calculations. The conical singularity gives rise to boundary terms residing on the entangling surface, which are usually discarded. Consistency with our results requires they be kept. We argue that, in fact, this conclusion also follows from the work of Metlitski, Fuertes, and Sachdev, which demonstrated that such boundary terms will be generated through quantum corrections.

  6. MetabR: an R script for linear model analysis of quantitative metabolomic data

    Directory of Open Access Journals (Sweden)

    Ernest Ben

    2012-10-01

    Full Text Available Abstract Background Metabolomics is an emerging high-throughput approach to systems biology, but data analysis tools are lacking compared to other systems level disciplines such as transcriptomics and proteomics. Metabolomic data analysis requires a normalization step to remove systematic effects of confounding variables on metabolite measurements. Current tools may not correctly normalize every metabolite when the relationships between each metabolite quantity and fixed-effect confounding variables are different, or for the effects of random-effect confounding variables. Linear mixed models, an established methodology in the microarray literature, offer a standardized and flexible approach for removing the effects of fixed- and random-effect confounding variables from metabolomic data. Findings Here we present a simple menu-driven program, “MetabR”, designed to aid researchers with no programming background in statistical analysis of metabolomic data. Written in the open-source statistical programming language R, MetabR implements linear mixed models to normalize metabolomic data and analysis of variance (ANOVA to test treatment differences. MetabR exports normalized data, checks statistical model assumptions, identifies differentially abundant metabolites, and produces output files to help with data interpretation. Example data are provided to illustrate normalization for common confounding variables and to demonstrate the utility of the MetabR program. Conclusions We developed MetabR as a simple and user-friendly tool for implementing linear mixed model-based normalization and statistical analysis of targeted metabolomic data, which helps to fill a lack of available data analysis tools in this field. The program, user guide, example data, and any future news or updates related to the program may be found at http://metabr.r-forge.r-project.org/.

  7. RG-Whitham dynamics and complex Hamiltonian systems

    Directory of Open Access Journals (Sweden)

    A. Gorsky

    2015-06-01

    Full Text Available Inspired by the Seiberg–Witten exact solution, we consider some aspects of the Hamiltonian dynamics with the complexified phase space focusing at the renormalization group (RG-like Whitham behavior. We show that at the Argyres–Douglas (AD point the number of degrees of freedom in Hamiltonian system effectively reduces and argue that anomalous dimensions at AD point coincide with the Berry indexes in classical mechanics. In the framework of Whitham dynamics AD point turns out to be a fixed point. We demonstrate that recently discovered Dunne–Ünsal relation in quantum mechanics relevant for the exact quantization condition exactly coincides with the Whitham equation of motion in the Ω-deformed theory.

  8. RG flows in the D-series of minimal CFTs

    International Nuclear Information System (INIS)

    Klassen, T.R.; Melzer, E.

    1993-01-01

    Using results of the thermodynamic Bethe ansatz approach and conformal perturbation theory we argue that the φ 1,3 perturbation of a unitary minimal (1+1)-dimensional conformal field theory (CFT) in the D-series of modular invariant partition functions induces a renormalization group (RG) flow to the next-lower model in the D-series. An exception is the first model in the series, the three-state Potts CFT, which under the Z 2 -even φ 1,3 -perturbation flows to the tricritical Ising CFT, the second model in the A-series. We present arguments that in the A-series flow corresponding to this exceptional case, interpolating between the tetracritical and the tricritical Ising CFT, the IR fixed point is approached from 'exactly the opposite direction'. Our results indicate how (most of) the relevant conformal fields evolve from the UV to the IR CFT. (orig.)

  9. Defects and boundary RG flows in ℂ/ℤ{sub d}

    Energy Technology Data Exchange (ETDEWEB)

    Becker, Melanie; Cabrera, Yaniel [George and Cynthia Mitchell Institute for Fundamental Physics and Astronomy,Texas A& M University,College Station, TX 77843-4242 (United States); Robbins, Daniel [Department of Physics, University at Albany,1400 Washington Ave., Albany, NY 12222 (United States)

    2017-02-01

    We show that topological defects in the language of Landau-Ginzburg models carry information about the RG flow between the non-compact orbifolds ℂ/ℤ{sub d}. We show that such defects correctly implement the bulk-induced RG flow on the boundary.

  10. Neuroprotective effects of ginsenoside Rg1 against oxygen–glucose deprivation in cultured hippocampal neurons

    Directory of Open Access Journals (Sweden)

    Qing He

    2014-03-01

    Conclusion: Ginsenoside Rg1 has neuroprotective effect on ischemia–reperfusion injury in cultured hippocampal cells mediated by blocking calcium over-influx into neuronal cells and decreasing the nNOS activity after OGD exposure. We infer that ginsenoside Rg1 may serve as a potential therapeutic agent for cerebral ischemia injury.

  11. El síndrome metabólico en adultos, en el Perú

    Directory of Open Access Journals (Sweden)

    Jaime Pajuelo

    2007-03-01

    Full Text Available Objetivo: Conocer la prevalencia del síndrome metabólico en la población adulta del Perú. Materiales y Métodos: Se estudió 4091 personas mayores de 20 años. El 50,4% correspondió al género femenino y 49,6% al masculino. Estas personas fueron elegidas sobre la base de un muestreo por conglomerado trietápico, que representa el nivel nacional y los siguientes ámbitos: Lima metropolitana, resto de la costa, sierra urbana, sierra rural y selva. A todos se les tomó el peso, la talla, la circunferencia de la cintura, la presión arterial, y se les dosó triglicéridos, colesterol HDL y glucosa. Para el diagnóstico del síndrome metabólico se utilizó el criterio del National Cholesterol Education Program ATP III (Adult Treatment Panel. Resultados: La prevalencia nacional del síndrome metabólico fue 16,8%. Lima metropolitana (20,7% y el resto de la costa (21,5% fueron los únicos ámbitos que estuvieron por encima de la prevalencia nacional. La sierra rural es la que presentó los valores más bajos, con 11,1%. El género femenino (26,4% superó ampliamente al masculino (7,2%. El síndrome metabólico fue más prevalente en las personas con obesidad que en las que tenían sobrepeso. A mayor edad, mayor presencia del síndrome metabólico. Conforme se incrementó la circunferencia de la cintura, las otras variables lo hicieron de la misma manera. Conclusiones: En el país, 2 680 000 personas presentaron el síndrome metabólico, lo que significa que una gran cantidad de personas se encuentra en riesgo de su salud por las diversas alteraciones que le pueden ocurrir. Conociendo que la principal causa de este problema es el sobrepeso y la obesidad, hay que realizar estrategias que permitan combatir lo mencionado. Estas estrategias son ampliamente conocidas: tener una alimentación saludable y realizar una actividad física.

  12. Relación entre el estado nutricional y el síndrome metabólico en adultos

    Directory of Open Access Journals (Sweden)

    María José Romero Ausfeld

    2015-08-01

    Full Text Available El síndrome metabólico constituye una agrupación de factores como son la obesidad abdominal, dislipidemia, glucemia y presión arterial elevadas, implicados en el desarrollo de la diabetes mellitus y enfermedades cardiovasculares. El estado nutricional refleja la condición física en la que se encuentra un individuo, evaluado comúnmente por el Índice de Masa Corporal (IMC o Índice de Quelet. El objetivo de este estudio fue evaluar la relación entre el estado nutricional y el síndrome metabólico en adultos de ambos sexos. Estudio transversal analítico con datos secundarios de sujetos que frecuentaron el parque de la salud del Instituto de Previsión Social (Asunción, Paraguay en el mes de setiembre del 2.011, realizado en 98 sujetos con rango de edad de 34 a 51 años. La prevalencia del síndrome metabólico fue del 25,5%. En mujeres con síndrome metabólico fueron más frecuentes los criterios de circunferencia de cintura (p<0,001 y triglicéridos (p<0,001 aumentados, en hombres el colesterol-HDL bajo (p=0,001, la glucemia (p=0,007 y presión arterial elevadas (p=0,002. El estado nutricional según índice de masa corporal indicó un mayor porcentaje de exceso de peso en hombres (89,6%. Al relacionar el estado nutricional con el síndrome metabólico, en general, la mayoría de los sujetos obesos presentaron síndrome metabólico (p<0,001; por sexo se observó que la mayoría de las mujeres tenían sobrepeso y que los hombres con obesidad presentaron un mayor porcentaje de síndrome metabólico (p=0,001. El estado nutricional y el síndrome metabólico se incrementan de manera proporcional, observándose una mayor prevalencia de síndrome metabólico en mujeres con sobrepeso y en hombres con obesidad. La prevalencia del síndrome metabólico fue del 25,5% siendo menor el síndrome metabólico premórbido (22,4%.

  13. Síndrome metabólica: identificando fatores de risco

    OpenAIRE

    Lottenberg,Simão Augusto; Glezer,Andrea; Turatti,Luiz Alberto

    2007-01-01

    OBJETIVOS Discutir a síndrome metabólica e a identificação de seus fatores de risco, inclusive na faixa etária pediátrica. FONTES DE DADOS: Artigos de revisão indexados. SÍNTESE DOS DADOS:A síndrome metabólica caracteriza-se pela resistência à insulina e pela presença de fatores de risco para doenças cardiovasculares e diabetes melito tipo 2. Não existe ainda um consenso com relação a seus critérios diagnósticos. Na presente revisão, os critérios diagnósticos da Associação Americana de Cardio...

  14. Factores de riesgo para sindrome metabólico en pacientes con higado graso.

    OpenAIRE

    Uzcátegui, Lilia; Ángel, José Gregorio; Martínez, Dasyl; Gómez Pérez, Roald; Arata de Bellabarba, Gabriela

    2008-01-01

    Editorial. Periodismo, mal periodismo, mal de Chagas y ciencias de la salud. Editorial. Journalism, poor journalim, Chagas disease and health sciences. Salinas, Pedro José Cistoadenoma seroso de ovario y embarazo. Reporte de caso. Ovarian serous cystoadenoma and pregnancy. Case report Terán, Penélope; Uribarri, Carolina; Castillo, Sonia y Salinas, Roberto. Factores de riesgo para sindrome metabólico en pacientes con higado graso. Risk factors of the metabolic syndrome i...

  15. Evaluación metabólica y nutricional en litiasis renal

    Directory of Open Access Journals (Sweden)

    B. Rodrigo Orozco, Dr.

    2010-07-01

    Full Text Available Los pacientes con litiasis renal requieren de investigaciones para identificar las condiciones médicas subyacentes y otras anomalías metabólicas predisponentes. Los resultados de estas investigaciones se utilizan para guiar el tratamiento preventivo. La profundidad del estudio necesario depende de varios factores, incluyendo la edad, la historia clínica de la persona y el número y la frecuencia de los cálculos. Una variedad de factores dietéticos y metabólicos pueden contribuir o causar la formación de litiasis renal. Los factores dietéticos incluyen una alta ingesta de proteínas animales, oxalato y sodio, y una baja ingesta de líquidos y de productos cítricos que contienen potasio. Las alteraciones metabólicas más frecuentemente asociadas a litiasis son la hipercalciuria, la hipocitraturia, la gota, la hiperoxaluria y la hiperuricosuria. Las modificaciones en la dieta deben aplicarse en todos los pacientes con litiasis renal, y consisten en una elevada ingesta de líquidos, la restricción de oxalato y sodio, una dieta balanceada en proteínas animales y complementadas por una ingesta adecuada de frutas y verduras. Cuando las modificaciones en la dieta no son suficientes en prevenir la formación de litiasis o en la presencia de alteraciones metabólicas importantes, es necesaria una intervención farmacológica especifica.

  16. Rg/sup a/ (Rodgers) and the HLA region: linkage and associations

    Energy Technology Data Exchange (ETDEWEB)

    Giles, C.M. (MRC Blood Group Reference Lab., London, Eng.); Gedde-Dahl, T. Jr.; Robson, E.B.; Thorsby, E.; Olaisen, B.; Arnason, A.; Kissmeyer-Nielsen, F.; Schreuder, I.

    1976-01-01

    In 19 families with 97 children the segregation of Rg/sup a/ (Rodgers) was found to be compatible with Mendelian inheritance and five backcross and 14 intercross families were found among HLA and BF type families. Close linkage (lods + 17.82) without recombination was found between Rg and the HLA region, with a direct count of 96 nonrecombinant meioses for Rg--HLA--B, Rg/sup -/ was strongly associated with HLA-B8 (29 of 30 haplotypes) and probably associated with Bw40, but did occur on other HLA--B haplotypes. By inference Rg/sup -/ is negatively associated with Ch/sup -/ (Chido). The Rg/sup -/Ch/sup -/ haplotype has not been observed. Rg/sup a/ and Ch/sup a/ may or may not be coded for by different sites of the same cistron closely linked to HLA--B:C and cannot as yet be excluded from being parts of B or C.

  17. La expresión neonatal de microRNAs como potenciales biomarcadores tempranos del síndrome metabólico

    OpenAIRE

    P. Rodil-García; L.A. Salazar-Olivo

    2015-01-01

    El síndrome metabólico es un trastorno caracterizado por obesidad, hipertensión arterial, hipercolesterolemia, hiperlipidemia y alteraciones en el metabolismo de la glucosa, y constituye un factor de riesgo para el desarrollo de diabetes y enfermedades cardiovasculares. Las condiciones nutricionales, hormonales y metabólicas de la madre durante el embarazo y el posparto temprano pueden condicionar el desarrollo de enfermedades metabólicas en la vida adulta de los hijos. Este concepto, llamado...

  18. Manejo integral del paciente hipertenso con síndrome metabólico y diabetes

    Directory of Open Access Journals (Sweden)

    Vicente Lahera, MD

    2010-01-01

    Full Text Available En los últimos años se ha observado un aumento de la prevalencia de obesidad y síndrome metabólico a nivel mundial, cuyas causas habrían de buscarse fundamentalmente en el sedentarismo y la alimentación inadecuada. La obesidad y el síndrome metabólico son una primera causa para el desarrollo de enfermedad cardiovascular y diabetes mellitus tipo 2. La resistencia a la insulina desempeña un papel central en el desarrollo de las alteraciones vasculares y metabólicas que acompañan al síndrome metabólico y la obesidad: dislipidemia aterogénica, hipertensión y estado protrombótico e inflamatorio. La activación del sistema nervioso simpático y del sistema reninaangiotensina- aldosterona también participa en la mayoría de estas alteraciones. Por ello, el bloqueo de las acciones de la angiotensina II con los inhibidores de la enzima convertidora de angiotensina (IECA o los antagonistas de los receptores de angiotensina II (ARA-II, se considera una aproximación terapéutica obligatoria en estos pacientes. Es preciso considerar que muchos pacientes con múltiples factores de riesgo requieren más de un fármaco para controlar su hipertensión. La combinación de medicamentos bloqueadores de la angiotensina II y diuréticos es la más frecuente; sin embargo, en pacientes hipertensos con diabetes, síndrome metabólico o alteraciones del metabolismo de la glucosa, ésta no parece ser la más adecuada debido al potencial diabetogénico de los diuréticos tiazídicos. Por ello, la unión de IECA y calcioantagonistas se ha revelado como una combinación alternativa interesante. Los estudios ACCOMPLISH, STAR y STAR-LET, han demostrado beneficios importantes en pacientes diabéticos o con resistencia a la insulina.

  19. Predicted NMR properties of noble gas hydride cations RgH +

    Science.gov (United States)

    Cukras, Janusz; Sadlej, Joanna

    2008-12-01

    The NMR shielding constants and, for the first time, the spin-spin coupling constants of Rg and H in RgH + compounds for Rg = Ne, Ar, Kr, Xe have been investigated by non-relativistic Hartree-Fock (HF) and relativistic Dirac-Hartree-Fock (DHF) methods. Electron-correlation effects have been furthermore calculated using SOPPA and CCSD at the non-relativistic level. The correlation effects are large on both parameters and opposite to the relativistic effects. The results indicate that both the relativistic and correlation effects need to be taken into account in a quantitative computations, especially in the case of the spin-spin coupling constants.

  20. Anatomy of new SUSY breaking holographic RG flows

    Energy Technology Data Exchange (ETDEWEB)

    Argurio, Riccardo [Physique Théorique et Mathématique andInternational Solvay Institutes, Université Libre de Bruxelles,C.P. 231, 1050 Brussels (Belgium); Musso, Daniele [International Center of Theoretical Physics (ICTP),Strada Costiera 11, I 34014 Trieste (Italy); Redigolo, Diego [Physique Théorique et Mathématique andInternational Solvay Institutes, Université Libre de Bruxelles,C.P. 231, 1050 Brussels (Belgium); Sorbonne Universités, UPMC University Paris 06, UMR 7589, LPTHE,F-75005, Paris (France); CNRS, UMR 7589, LPTHE,F-75005, Paris (France)

    2015-03-17

    We find and thoroughly study new supergravity domain wall solutions which are holographic realizations of supersymmetry breaking strongly coupled gauge theories. We set ourselves in an N=2 gauged supergravity with a minimal content in order to reproduce a dual N=1 effective SCFT which has a U(1){sub R} symmetry, a chiral operator whose components are responsible for triggering the RG flow, and an additional U(1){sub F} symmetry. We present a full three dimensional parameter space of solutions, which generically break supersymmetry. Some known solutions are recovered for specific sets of values of the parameters, with the new solutions interpolating between them. The generic backgrounds being singular, we provide a stability test of their dual theories by showing that there are no tachyonic resonances in the two point correlators. We compute the latter by holographic renormalization. We also carefully analyze the appearance of massless modes, such as the dilaton and the R axion, when the respective symmetries are spontaneously broken, and their lifting when the breaking is explicit. We further comment on the application of such class of backgrounds as archetypes of strongly coupled hidden sectors for gauge mediation of supersymmetry breaking. In particular, we show that it is possible to model in this way all types of hierarchies between the visible sector gaugino and sfermion masses.

  1. Static electric polarizabilities and first hyperpolarizabilities of molecular ions RgH + (Rg = He, Ne, Ar, Kr, Xe): ab initio study

    Science.gov (United States)

    Cukras, Janusz; Antušek, Andrej; Holka, Filip; Sadlej, Joanna

    2009-06-01

    Extensive ab initio calculations of static electric properties of molecular ions of general formula RgH + (Rg = He, Ne, Ar, Kr, Xe) involving the finite field method and coupled cluster CCSD(T) approach have been done. The relativistic effects were taken into account by Douglas-Kroll-Hess approximation. The numerical stability and reliability of calculated values have been tested using the systematic sequence of Dunning's cc-pVXZ-DK and ANO-RCC-VQZP basis sets. The influence of ZPE and pure vibrational contribution has been discussed. The component αzz has increasing trend in RgH + while the relativistic effect on αzz leads to a small increase of this molecular parameter.

  2. The holographic RG flow in a field theory on a curved background

    International Nuclear Information System (INIS)

    Cardoso, Gabriel Lopes; Luest, Dieter

    2002-01-01

    As shown by Freedman, Gubser, Pilch and Warner, the RG flow in N=4 super-Yang-Mills theory broken to an N=1 theory by the addition of a mass term can be described in terms of a supersymmetric domain wall solution in five-dimensional N=8 gauged supergravity. The FGPW flow is an example of a holographic RG flow in a field theory on a flat background. Here we put the field theory studied by Freedman, Gubser, Pilch and Warner on a curved AdS 4 background, and we construct the supersymmetric domain wall solution which describes the RG flow in this field theory. This solution is a curved (non-Ricci flat) domain wall solution. This example demonstrates that holographic RG flows in supersymmetric field theories on a curved AdS 4 background can be described in terms of curved supersymmetric domain wall solutions. (author)

  3. Bouncing and emergent cosmologies from Arnowitt–Deser–Misner RG flows

    Science.gov (United States)

    Bonanno, Alfio; Gionti, S. J. Gabriele; Platania, Alessia

    2018-03-01

    Asymptotically safe gravity provides a framework for the description of gravity from the trans-Planckian regime to cosmological scales. According to this scenario, the cosmological constant and Newton’s coupling are functions of the energy scale whose evolution is dictated by the renormalization group (RG) equations. The formulation of the RG equations on foliated spacetimes, based on the Arnowitt–Deser–Misner (ADM) formalism, furnishes a natural way to construct the RG energy scale from the spectrum of the Laplacian operator on the spatial slices. Combining this idea with an RG improvement procedure, in this work we study quantum gravitational corrections to the Einstein–Hilbert action on Friedmann–Lemaître–Robertson–Walker backgrounds. The resulting quantum-corrected Friedmann equations can give rise to both bouncing cosmologies and emergent Universe solutions. Our bouncing models do not require the presence of exotic matter and emergent Universe solutions can be constructed for any allowed topology of the spatial slices.

  4. Expression of the rgMT gene, encoding for a rice metallothionein ...

    Indian Academy of Sciences (India)

    the rice rgMT gene in transgenic yeast and Arabidopsis is implicated in improving their tolerance for .... possible by the use of a microscope with UV optics that ... Abiotic stress factors that induced the expression of the ..... Arabidopsis mutant.

  5. Exercício físico e síndrome metabólica Ejercicio físico y síndrome metabólico Physical exercise and metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Emmanuel Gomes Ciolac

    2004-08-01

    Full Text Available A prática regular de atividade física tem sido recomendada para a prevenção e reabilitação de doenças cardiovasculares e outras doenças crônicas por diferentes associações de saúde no mundo, como o American College of Sports Medicine, os Centers for Disease Control and Prevention, a American Heart Association, o National Institutes of Health, o US Surgeon General, a Sociedade Brasileira de Cardiologia, entre outras. Estudos epidemiológicos têm demonstrado relação direta entre inatividade física e a presença de múltiplos fatores de risco como os encontrados na síndrome metabólica. Entretanto, tem sido demonstrado que a prática regular de exercício físico apresenta efeitos benéficos na prevenção e tratamento da hipertensão arterial, resistência à insulina, diabetes, dislipidemia e obesidade. Com isso, o condicionamento físico deve ser estimulado para todos, pessoas saudáveis e com múltiplos fatores de risco, desde que sejam capazes de participar de um programa de treinamento físico. Assim como a terapêutica clínica cuida de manter a função dos órgãos, a atividade física promove adaptações fisiológicas favoráveis, resultando em melhora da qualidade de vida.La práctica regular de actividad fisica viene siendo recomendada para la prevención y la rehabilitación de enfermedades cardiovasculares y otras dolencias crónicas por las diferentes asociaciones de salud del mundo, como el Colegio Americano de Medicina del Deporte, los Centros para el Control y Prevención de Enfermedades, la Asociación Cardiológica Americana, los Institutos Nacionales de Salud, el US Surgeon General y la Sociedad Brasileña de Cardiología entre otras. Estudios epidemiológicos han demostrado que la relación directa entre la inactividad física y la presencia de múltiples factores de riesgo como los encontrados en el síndrome metabólico. Entretanto, se ha demostrado que la práctica regular del ejercicio físico presenta

  6. Pivotal Roles of Ginsenoside Rg3 in Tumor Apoptosis Through Regulation of Reactive Oxygen Species.

    Science.gov (United States)

    Sun, Hwa Yeon; Lee, Jun Hee; Han, Yong-Seok; Yoon, Yeo Min; Yun, Chul Won; Kim, Jae Heon; Song, Yun Seob; Lee, Sang Hun

    2016-09-01

    Elevated production of reactive oxygen species (ROS) is observed in various cancer types and pathophysiological conditions. In cancer cells, ROS induce cell proliferation, genetic instability, and a malignant phenotype. Ginsenoside Rg3 is the main pharmacologically active component in ginseng and has been reported to have an antioxidant effect. To overcome lung cancer by regulating the ROS level, we investigated the antitumor effect and mechanism of Rg3 and its antioxidative property on Lewis lung carcinoma (LLC) cells. Inhibition of ROS was suppressed in LLC cells by Rg3 treatment, and these cells were used to investigate the antioxidant, antiproliferative, and antitumor effects in LLC cells. ROS production was increased in cells grown in serum-containing media (conditioned media) compared to those grown in serum-free media. The high level of ROS induced LLC cell proliferation, but treatment with Rg3 (200 ng/ml) resulted in reduction of ROS, leading to inhibition of cell proliferation. Treatment with Rg3 significantly reduced cyclin and cyclin-dependent kinase expression in LLC cells. Additionally, Rg3 treatment significantly suppressed activation of mitogen-activated protein kinases and induced LLC cell apoptosis through activation of pro-apoptotic proteins and suppression of anti-apoptotic proteins. Taken together, these findings demonstrate the role of Rg3 in reduction of the intracellular ROS level, attenuation of proliferation via augmentation of cell cycle- and cell proliferation-associated proteins, and activation of apoptosis through regulation of apoptosis-associated proteins in LLC. These findings suggest that Rg3 could be used as a therapeutic agent in lung cancer. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  7. Ginsenoside Rg1 improves bone marrow haematopoietic activity via extramedullary haematopoiesis of the spleen.

    Science.gov (United States)

    Liu, Hua-Hsing; Chen, Fei-Peng; Liu, Rong-Kai; Lin, Chun-Lin; Chang, Ko-Tung

    2015-11-01

    Cyclophosphamide (CY) is a chemotherapeutic agent used for cancer and immunological diseases. It induces cytotoxicity of bone marrow and causes myelosuppression and extramedullary haematopoiesis (EMH) in treated patients. EMH is characterized with the emergence of multipotent haematopoietic progenitors most likely in the spleen and liver. Previous studies indicated that a Chinese medicine, ginsenoside Rg1, confers a significant effect to elevate the number of lineage (Lin(-) ) Sca-1(+) c-Kit(+) haematopoietic stem and progenitor cells (HSPCs) and restore the function of bone marrow in CY-treated myelosuppressed mice. However, whether the amelioration of bone marrow by Rg1 accompanies an alleviation of EMH in the spleen was still unknown. In our study, the cellularity and weight of the spleen were significantly reduced after Rg1 treatment in CY-treated mice. Moreover, the number of c-Kit(+) HSPCs was significantly decreased but not as a result of apoptosis, indicating that Rg1 alleviated EMH of the spleen induced by CY. Unexpectedly, the proliferation activity of c-Kit(+) HSPCs was only up-regulated in the spleen, but not in the bone marrow, after Rg1 treatment in CY-treated mice. We also found that a fraction of c-Kit(+) /CD45(+) HSPCs was simultaneously increased in the circulation after Rg1 treatment. Interestingly, the effects of Rg1 on the elevation of HSPCs in bone marrow and in the peripheral blood were suppressed in CY-treated splenectomized mice. These results demonstrated that Rg1 improves myelosuppression induced by CY through its action on the proliferation of HSPCs in EMH of the spleen and migration of HSPCs from the spleen to the bone marrow. © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

  8. Ginsenoside Rg1 ameliorates hippocampal long-term potentiation and memory in an Alzheimer's disease model.

    Science.gov (United States)

    Li, Fengling; Wu, Xiqing; Li, Jing; Niu, Qingliang

    2016-06-01

    The complex etiopathogenesis of Alzheimer's disease (AD) has limited progression in the identification of effective therapeutic agents. Amyloid precursor protein (APP) and presenilin‑1 (PS1) are always overexpressed in AD, and are considered to be the initiators of the formation of β‑amyloid plaques and the symptoms of AD. In the present study, a transgenic AD model, constructed via the overexpression of APP and PS1, was used to verify the protective effects of ginsenoside Rg1 on memory performance and synaptic plasticity. AD mice (6‑month‑old) were treated via intraperitoneal injection of 0.1‑10 mg/kg ginsenoside Rg1. Long‑term memory, synaptic plasticity, and the levels of AD‑associated and synaptic plasticity‑associated proteins were measured following treatment. Memory was measured using a fear conditioning task and protein expression levels were investigated using western blotting. All the data was analyzed by one-way analysis of variance or t‑test. Following 30 days of consecutive treatment, memory in the AD mouse model was ameliorated in the 10 mg/kg ginsenoside Rg1 treatment group. As demonstrated by biochemical experiments, ginsenoside Rg1 treatment reduced the accumulations of β‑amyloid 1‑42 and phosphorylated (p)‑Tau in the AD model. Additionally, brain-derived neurotrophic factor (BDNF) and p‑TrkB synaptic plasticity‑associated proteins were upregulated following ginsenoside Rg1 application. Correspondingly, long‑term potentiation (LTP) was restored following ginsenoside Rg1 application in the AD mice model. Taken together, ginsenoside Rg1 repaired hippocampal LTP and memory, likely through facilitating the clearance of AD‑associated proteins and through activation of the BDNF‑TrkB pathway. Therefore, ginsenoside Rg1 may be a candidate drug for the treatment of AD.

  9. Neuroprotective effects of ginsenoside Rg1-induced neural stem cell transplantation on hypoxic-ischemic encephalopathy

    Directory of Open Access Journals (Sweden)

    Ying-bo Li

    2015-01-01

    Full Text Available Ginsenoside Rg1 is the major pharmacologically active component of ginseng, and is reported to have various therapeutic actions. To determine whether it induces the differentiation of neural stem cells, and whether neural stem cell transplantation after induction has therapeutic effects on hypoxic-ischemic encephalopathy, we cultured neural stem cells in 10-80 µM ginsenoside Rg1. Immunohistochemistry revealed that of the concentrations tested, 20 mM ginsenoside Rg1 had the greatest differentiation-inducing effect and was the concentration used for subsequent experiments. Whole-cell patch clamp showed that neural stem cells induced by 20 µM ginsenoside Rg1 were more mature than non-induced cells. We then established neonatal rat models of hypoxic-ischemic encephalopathy using the suture method, and ginsenoside Rg1-induced neural stem cells were transplanted via intracerebroventricular injection. These tests confirmed that neural stem cells induced by ginsenoside had fewer pathological lesions and had a significantly better behavioral capacity than model rats that received saline. Transplanted neural stem cells expressed neuron-specific enolase, and were mainly distributed in the hippocampus and cerebral cortex. The present data suggest that ginsenoside Rg1-induced neural stem cells can promote the partial recovery of complicated brain functions in models of hypoxic-ischemic encephalopathy.

  10. Indicadores metabólicos en razas lecheras especializadas en condiciones tropicales en Colombia

    Directory of Open Access Journals (Sweden)

    Rómulo Campos G

    2007-06-01

    Full Text Available Se ha seleccionado un alto número de razas bovinas para producción de leche, sin embargo, las de origen Bos taurus no han logrado adaptarse a las condiciones tropicales. El objetivo del trabajo fue analizar el comportamiento metabólico de siete razas (Ayrshire, Girolando, Holstein Friesian, Jersey, Lucerna, Pardo Suizo y Simenthal a través de 15 metabolitos. Se emplearon 28 animales por raza, distribuidos en cuatro grupos fisiológicos: novillas, inicio y final de lactancia y vacas secas (final de gestación. Los valores medios de los indicadores metabólicos fueron: BOH 0.5 mmol/l; glucosa 2.8 mmol/l; colesterol 2.5 mmol/l; potasio 4.1 mmol/l; calcio 2.0 mmol/l; fósforo inorgánico 1.7 mmol/l; magnesio 1.1 mmol/l; proteínas totales 66.2 mg/dl; albúmina 25.8 mg/dl; globulinas 40.2 mg/dl; creatinina 109 µmol/l; BUN 3.8 mmol/l; ALT 32.2 UI/l; AST 56.6 UI/l; GGT 12.3 UI/l; bilirrubina total 0.2 µmol/l; bilirrubina conjugada 0.08 µmol/l. Los valores medios de la condición corporal y el hematocrito fueron 3.25% y 27.0% respectivamente. Se encontraron diferencias estadísticas significativas entre grupos raciales y entre grupos de producción. Los animales de menor peso metabólico (Jersey presentaron mejor homeostasis que los de pesos mayores (Simenthal, Holstein.

  11. Síndrome metabólica: importância de seu reconhecimento

    OpenAIRE

    Sthefano Atique Gabriel; Cristiane Knopp Tristão; José Roberto Pretel Pereira Job; Antonio Carlos Guerra da Cunha

    2007-01-01

    A Síndrome Metabólica caracteriza-se por um agrupamento de fatores de risco para doenças cardiovasculares e diabetes, geralmente ligados à resistência à insulina, à obesidade e à obesidade central, tais como, hipertensão arterial sistêmica, obesidade abdominal, tolerância à glicose prejudicada, hipertrigliceridemia e baixas concentrações sanguíneas de HDL-colesterol, além dos estados pró-trombótico e pró-inflamatório observados. Uma alimentação adequada, entretanto, associada a modificações n...

  12. El síndrome metabólico ¿Cómo abordar el problema?

    Directory of Open Access Journals (Sweden)

    Alain Francisco Morejón Giraldoni

    2011-08-01

    Full Text Available El síndrome metabólico (SM es una entidad clínica cuya prevalencia mundial se incrementa año tras año, lo que lo convierte en un serio problema de salud pública. Se considera que este aumento se debe, por un lado, al estilo de vida sedentario, y por el otro, a la obesidad, por lo que se asocia con un incremento del riesgo de padecer enfermedad cardiovascular (ECV y diabetes (DM tipo II.

  13. Efeito do yacon (Smallanthus sonchifolius) e Lactobacillus plantarum sobre os parâmetros metabólicos e de estresse oxidativo em ratos Wistar com síndrome metabólica

    OpenAIRE

    Naiara Lourenço Mari

    2015-01-01

    A Síndrome metabólica (SM) é caracterizada pela associação de fatores de riscos para doenças cardiovasculares e Diabetes Mellitus tipo 2, tais como adiposidade abdominal, resistência à insulina, dislipidemia, hipertensão e disfunção endotelial. A presente investigação avaliou o efeito do prebiótico yacon (Smallanthus sonchifolius), e do probiótico Lactobacillus plantarum Lp115, individualmente e em simbiose, nos parâmetros metabólicos e de estresse oxidativo em ratos com SM. A SM foi induzida...

  14. Theoretical Study of Group 14 M^{+}(^{2}P_{J})-RG Complexes (M^{+} = C^{+}, Si^{+}; RG = he - Ar)

    Science.gov (United States)

    Tuttle, William Duncan; Thorington, Rebecca L.; Wright, Timothy G.; Viehland, Larry A.

    2017-06-01

    The light group 14 cations are found in a wide variety of environments, with, for example, C^{+} ions thought to play a key role in the chemistry of the interstellar medium, while Si^{+} ions are an important component of the upper atmosphere of the Earth due to their presence in meteoroids. We calculate accurate interatomic potentials for a singly charged carbon cation and a singly charged silicon cation interacting with the rare gas atoms helium, neon and argon. The RCCSD(T) method is employed, with basis sets of quadruple-ζ and quintuple-ζ quality, and the energies counterpoise corrected and extrapolated to the basis set limit at each point. In all cases, we consider the lowest electronic states of the M^{+} atom, (^{2}P_{J}), interacting with the ground electronic state of the RG atom, (^{1}S_{0}), and compute potentials corresponding to the molecular terms, ^{2}Π and ^{2}Σ^{+}, as well as the spin-orbit levels which arise: ^{2}Π_{3/2}, ^{2}Π_{1/2} and ^{2}Σ_{1/2}^{+}. The potentials are employed to calculated spectroscopic constants and ion transport properties. S. Petrie and D. K. Bohme, Mass Spec. Rev., 26, 258 (2007). J. M. C. Plane, J. C. Gómez-Martin, W. Feng, and D. Janches, J. Geophys. Res. Atmos. 121, 3718 (2016). W. D. Tuttle, R. L. Thorington, L. A. Viehland and T. G. Wright, Mol. Phys. 113, 3767 (2015). W. D. Tuttle, R. L. Thorington, L. A. Viehland and T. G. Wright (in preparation). W. D. Tuttle, R. L. Thorington, L. A. Viehland and T. G. Wright, Mol. Phys. 115, 437 (2017).

  15. Prevalencia del síndrome metabólico en profesores de Guanajuato, México

    Directory of Open Access Journals (Sweden)

    Francisco José Díaz-Cisneros

    2010-04-01

    Full Text Available Objetivos: Identificar la prevalencia del síndrome metabólico en profesores del nivel educativo básico del estado de Guanajuato, México. Diseño: Estudio observacional, transversal, retrospectivo. Institución: Universidad de Guanajuato, México. Participantes: Docentes de nivel educativo básico. Intervenciones: En 477 docentes, 208 hombres y 269 mujeres, se llevó a cabo una evaluación médica directa y exámenes de laboratorio de los componentes del síndrome metabólico (glucosa, triglicéridos y colesterol de densidad alta (HDL-C, así como, medición de circunferencia de abdomen, presión arterial y nivel de actividad física. Principales medidas de resultados: Prevalencia del síndrome metabólico. Resultados: La prevalencia del síndrome metabólico fue 32,2% en los docentes hombres y 26,8% en las mujeres. Más de 40% de los profesores presentó tres factores de riesgo para el síndrome metabólico, siendo más prevalente la hipertrigliceridemia. Referente al nivel de actividad física, 24% correspondió a personas sedentarias, 65% hipoactivos y únicamente 11% practicaba ejercicio vigoroso regular. Conclusiones: El perfil de salud de los docentes de Guanajuato se encontró lejos del ideal esperado, observándose una elevada prevalencia del síndrome metabólico, por lo que se sugiere aplicar estrategias de prevención para modificar los estilos de vida del profesorado, en especial mejorar la dieta y promover mayor nivel de actividad física.

  16. Exact results and open questions in first principle functional RG

    International Nuclear Information System (INIS)

    Le Doussal, Pierre

    2010-01-01

    Some aspects of the functional RG (FRG) approach to pinned elastic manifolds (of internal dimension d) at finite temperature T > 0 are reviewed and reexamined in this much expanded version of Le Doussal (2006) . The particle limit d = 0 provides a test for the theory: there the FRG is equivalent to the decaying Burgers equation, with viscosity ν ∼ T-both being formally irrelevant. An outstanding question in FRG, i.e. how temperature regularizes the otherwise singular flow of T = 0 FRG, maps to the viscous layer regularization of inertial range Burgers turbulence (i.e. to the construction of the inviscid limit). Analogy between Kolmogorov scaling and FRG cumulant scaling is discussed. First, multi-loop FRG corrections are examined and the direct loop expansion at T > 0 is shown to fail already in d = 0, a hierarchy of ERG equations being then required (introduced in Balents and Le Doussal (2005) ). Next we prove that the FRG function R(u) and higher cumulants defined from the field theory can be obtained for any d from moments of a renormalized potential defined in an sliding harmonic well. This allows to measure the fixed point function R(u) in numerics and experiments. In d = 0 the beta function (of the inviscid limit) is obtained from first principles to four loop. For Sinai model (uncorrelated Burgers initial velocities) the ERG hierarchy can be solved and the exact function R(u) is obtained. Connections to exact solutions for the statistics of shocks in Burgers and to ballistic aggregation are detailed. A relation is established between the size distribution of shocks and the one for droplets. A droplet solution to the ERG functional hierarchy is found for any d, and the form of R(u) in the thermal boundary layer is related to droplet probabilities. These being known for the d = 0 Sinai model the function R(u) is obtained there at any T. Consistency of the ε=4-d expansion in one and two loop FRG is studied from first principles, and connected to shock and

  17. Síndrome metabólico: concepto y aplicación práctica

    OpenAIRE

    Lizarzaburu Robles, Juan Carlos

    2014-01-01

    El síndrome metabólico (SM) es un conjunto de anormalidades metabólicas consideradas como un factor de riesgo para desarrollar enfermedad cardiovascular y diabetes. Los componentes del SM se han definido según diferentes guías y consensos. Las definiciones propuestas por el National Cholesterol Education Program Adult Treatment Panel III (ATP III) y la International Diabetes Federation (IDF) son las más utilizadas en las diferentes publicaciones; sin embargo, se han realizado actualizaciones ...

  18. Síndrome metabólico: concepto y aplicación práctica

    OpenAIRE

    Juan Carlos Lizarzaburu Robles

    2013-01-01

    El síndrome metabólico (SM) es un conjunto de anormalidades metabólicas consideradas como un factor de riesgo para desarrollar enfermedad cardiovascular y diabetes. Los componentes del SM se han definido según diferentes guías y consensos. Las definiciones propuestas por el National Cholesterol Education Program Adult Treatment Panel III (ATP III) y la International Diabetes Federation (IDF) son las más utilizadas en las diferentes publicaciones; sin embargo, se han realizado actualizaciones ...

  19. Protocolo Multiprofissional em cirúrgia bariátrica e metabólica com ênfase em fisioterapia

    OpenAIRE

    Nassif, Denise Serpa Bopp

    2011-01-01

    Resumo: OBJETIVOS: 1) criar base teórica de dados clínicos referentes a multiprofissionalidade em Cirurgia Bariátrica e Metabólica; 2) informatizar a base teórica dos dados transformando-os em protocolo eletrônico multiprofissional; 3) implantar a base teórica de dados clínicos no protocolo mestre e confeccionar o protocolo específico; 4) incorporar a base de dados ao SINPE©; 5) aplicar o protocolo eletrônico específico de Fisioterapia em Cirurgia Bariátrica e Metabólica; 6) analisar e compar...

  20. Disease: H01483 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available atin analog responsiveness in acromegaly. ... JOURNAL ... J Clin Endocrinol Metab 90:6290-5 (2005) DOI:10.1210/j...gland: New consensus in acromegaly: criteria for cure and control. ... JOURNAL ... Nat Rev Endocrinol 6:480-1 (2...c.2005-0998 ... PMID:20720589 (marker) ... AUTHORS ... Biermasz N ... TITLE ... Pituitary

  1. Genetics Home Reference: Allan-Herndon-Dudley syndrome

    Science.gov (United States)

    ... cells in the developing brain. This hormone, called triiodothyronine or T3, is produced by a butterfly-shaped ... with X-linked psychomotor retardation and elevated serum triiodothyronine. J Clin Endocrinol Metab. 2007 Jun;92(6): ...

  2. [Research of anti-aging mechanism of ginsenoside Rg1 on brain].

    Science.gov (United States)

    Li, Cheng-peng; Zhang, Meng-si; Liu, Jun; Geng, Shan; Li, Jing; Zhu, Jia-hong; Zhang, Yan-yan; Jia, Yan-yan; Wang, Lu; Wang, Shun-he; Wang, Ya-ping

    2014-11-01

    Neurodegenerative disease is common and frequently occurs in elderly patients. Previous studies have shown that ginsenoside Rg1 was able to inhibit senescent of brain, but the mechanism on the brain during the treatment remains elucidated. To study the mechanism of ginsenoside Rg1 in the process of anti-aging of brain, forty male SD rats were randomly divided into normal group, Rg1 normal group, brain aging model group and Rg1 brain aging model group, each group with 10 rats (brain aging model group: subcutaneous injection of D-galactose (120 mg kg(-1)), qd for 42 consecutive days; Rg1 brain aging model group: while copying the same test as that of brain aging model group, begin intraperitoneal injection of ginsenosides Rg1 (20 mg x kg(-1)) qd for 27 d from 16 d. Rg1 normal group: subcutaneous injection of the same amount of saline; begin intraperitoneal injection of ginsenosides Rg1 (20 mg x kg(-1)) qd for 27 d from 16 d. Normal: injected with an equal volume of saline within the same time. Perform the related experiment on the second day after finishing copying the model or the completion of the first two days of drug injections). Learning and memory abilities were measured by Morris water maze. The number of senescent cells was detected by SA-beta-Gal staining while the level of IL-1 and IL-6 proinflammatory cytokines in hippocampus were detected by ELISA. The activities of SOD, contents of GSH in hippo- campus were quantified by chromatometry. The change of telomerase activities and telomerase length were performed by TRAP-PCR and southern blotting assay, respectively. It is pointed that, in brain aging model group, the spatial learning and memory capacities were weaken, SA-beta-Gal positive granules increased in section of brain tissue, the activity of antioxidant enzyme SOD and the contents of GSH decreased in hippocampus, the level of IL-1 and IL-6 increased in hippocampus, while the length of telomere and the activity of telomerase decreased in hippocampus

  3. Ginsenoside Rg3 regulates S-nitrosylation of the NLRP3 inflammasome via suppression of iNOS

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Sung-Jin; Park, Jun-Young [Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon (Korea, Republic of); Department of Functional Genomics, University of Science and Technology, Yuseong-gu, Daejeon (Korea, Republic of); Choi, Song [Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon (Korea, Republic of); Lee, Jin-Bong; Jung, Haiyoung; Kim, Tae-Don; Yoon, Suk Ran; Choi, Inpyo [Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon (Korea, Republic of); Department of Functional Genomics, University of Science and Technology, Yuseong-gu, Daejeon (Korea, Republic of); Shim, Sungbo, E-mail: sungbo@ulsan.ac.kr [Department of Biomedical Sciences & Neuromarker Resource Bank (NRB), University of Ulsan College of Medicine, Seoul 138-736 (Korea, Republic of); Park, Young-Jun, E-mail: pyj71@kribb.re.kr [Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon (Korea, Republic of); Department of Functional Genomics, University of Science and Technology, Yuseong-gu, Daejeon (Korea, Republic of)

    2015-08-07

    Ginsenoside Rg3, a specific biological effector, is well-known as a major bioactive ingredient of Panax ginseng. However, its role in the inflammasome activation process remains unclear. In this report, we demonstrate that ginsenosides 20(R)-Rg3 and 20(S)-Rg3 are capable of suppressing both lethal endotoxic shock and the S-nitrosylation of the NLRP3 inflammasome by inhibiting nitric oxide (NO) production through the regulation of inducible nitric oxide synthase (iNOS) expression. In response to lipopolysaccharide (LPS), the reducing effect of 20(S)-Rg3 and 20(R)-Rg3 on nitric oxide led to an increase in the survival time of mice after lethal endotoxin-induced shock, and excess levels of NO inhibited IL-1β production via the S-nitrosylation of the NLRP3 inflammasome. In addition, ginsenosides 20(R)-Rg3 and 20(S)-Rg3 had suppressive effects on the LPS- or UV-irradiation-induced reactive oxygen species (ROS) levels in macrophage and HaCaT cells and thereby prevented apoptosis of spleen cells in mice. Altogether, these results demonstrate that ginsenoside 20(R)-Rg3 and 20(S)-Rg3, a naturally occurring compound, might act as a dual therapeutic regulator for the treatment of inflammatory and oxidative stress-related diseases. - Highlights: • Ginsenosides Rg3 inhibits NO production through the regulation of iNOS expression. • Ginsenosides Rg3 inhibits the S-nitrosylation of the NLRP3 inflammasome. • Ginsenosides Rg3 suppress on the LPS- or UV-irradiation-induced ROS levels in cells.

  4. Functional perturbative RG and CFT data in the ε-expansion

    Energy Technology Data Exchange (ETDEWEB)

    Codello, A. [Southern Denmark Univ., Odense (Denmark). CP3-Origins; INFN-Sezione di Bologna, Bologna (Italy); Safari, M. [INFN-Sezione di Bologna, Bologna (Italy); Bologna Univ. (Italy). Dipt di Fisica e Astronomia; Vacca, G.P. [INFN-Sezione di Bologna, Bologna (Italy); Zanusso, O. [INFN-Sezione di Bologna, Bologna (Italy); Jena Univ. (Germany). Theoretisch-Physikalisches Inst.

    2018-01-15

    We show how the use of standard perturbative RG in dimensional regularization allows for a renormalization group-based computation of both the spectrum and a family of coefficients of the operator product expansion (OPE) for a given universality class. The task is greatly simplified by a straightforward generalization of perturbation theory to a functional perturbative RG approach. We illustrate our procedure in the ε-expansion by obtaining the next-to-leading corrections for the spectrum and the leading corrections for the OPE coefficients of Ising and Lee-Yang universality classes and then give several results for the whole family of renormalizable multi-critical models φ{sup 2n}. Whenever comparison is possible our RG results explicitly match the ones recently derived in CFT frameworks. (orig.)

  5. Inequalities for trace anomalies, length of the RG flow, distance between the fixed points and irreversibility

    International Nuclear Information System (INIS)

    Anselmi, Damiano

    2004-01-01

    I discuss several issues about the irreversibility of the RG flow and the trace anomalies c, a and a'. First I argue that in quantum field theory: (i) the scheme-invariant area Δ a' of the graph of the effective beta function between the fixed points defines the length of the RG flow; (ii) the minimum of Δ a' in the space of flows connecting the same UV and IR fixed points defines the (oriented) distance between the fixed points and (iii) in even dimensions, the distance between the fixed points is equal to Δ a = a UV - a IR . In even dimensions, these statements imply the inequalities 0 ≤ Δ a ≤ Δ a' and therefore the irreversibility of the RG flow. Another consequence is the inequality a ≤ c for free scalars and fermions (but not vectors), which can be checked explicitly. Secondly, I elaborate a more general axiomatic set-up where irreversibility is defined as the statement that there exist no pairs of non-trivial flows connecting interchanged UV and IR fixed points. The axioms, based on the notions of length of the flow, oriented distance between the fixed points and certain 'oriented-triangle inequalities', imply the irreversibility of the RG flow without a global a function. I conjecture that the RG flow is also irreversible in odd dimensions (without a global a function). In support of this, I check the axioms of irreversibility in a class of d = 3 theories where the RG flow is integrable at each order of the large N expansion

  6. Ginsenoside Rg1 alleviates corticosterone-induced dysfunction of gap junctions in astrocytes.

    Science.gov (United States)

    Xia, Cong-Yuan; Chu, Shi-Feng; Zhang, Shuai; Gao, Yan; Ren, Qian; Lou, Yu-Xia; Luo, Piao; Tian, Man-Tong; Wang, Zhi-Qi; Du, Guo-Hua; Tomioka, Yoshihisa; Yamakuni, Tohru; Zhang, Yi; Wang, Zhen-Zhen; Chen, Nai-Hong

    2017-08-17

    Ginsenoside Rg1 (Rg1), one of the major bioactive ingredients of Panax ginseng C. A. Mey, has neuroprotective effects in animal models of depression, but the mechanism underlying these effects is still largely unknown AIM OF THE STUDY: Gap junction intercellular communication (GJIC) dysfunction is a potentially novel pathogenic mechanism for depression. Thus, we investigated that whether antidepressant-like effects of Rg1 were related to GJIC. Primary rat prefrontal cortical and hippocampal astrocytes cultures were treated with 50μM CORT for 24h to induce gap junction damage. Rg1 (0.1, 1, or 10μM) or fluoxetine (1μM) was added 1h prior to CORT treatment. A scrape loading and dye transfer assay was performed to identify the functional capacity of gap junctions. Western blot was used to detect the expression and phosphorylation of connexin43 (Cx43), the major component of gap junctions. Treatment of primary astrocytes with CORT for 24h inhibited GJIC, decreased total Cx43 expression, and increased the phosphorylation of Cx43 at serine368 in a dose-dependent manner. Pre-treatment with 1μM and 10μM Rg1 significantly improved GJIC in CORT-treated astrocytes from the prefrontal cortex and hippocampus, respectively, and this was accompanied by upregulation of Cx43 expression and downregulation of Cx43 phosphorylation. These findings provide the first evidence indicating that Rg1 can alleviate CORT-induced gap junction dysfunction, which may have clinical significance in the treatment of depression. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  7. Síndrome metabólico en niños y adolescentes hipertensos obesos

    Directory of Open Access Journals (Sweden)

    Yudalvis Oquendo de la Cruz

    2010-12-01

    Full Text Available INTRODUCCIÓN. La combinación de hipertensión y obesidad en la infancia y la adolescencia incrementa el riesgo de un síndrome metabólico (SM en edades tempranas de la vida. El objetivo de este estudio fue determinar la presencia de SM en adolescentes hipertensos obesos y su relación con algunas variantes clínicas y bioquímicas. MÉTODOS. Se realizó un estudio descriptivo y prospectivo, con 152 niños y adolescentes hipertensos obesos, con edades entre 5 y 19 años, procedentes de la consulta de nefrología del Hospital Pediátrico Universitario «Juan Manuel Márquez». Se realizaron estudios de microalbuminuria, glucemia, insulina, colesterol total y triglicéridos en ayunas. RESULTADOS. Se encontró síndrome metabólico en el 35,5 % de los hipertensos obesos y fue más frecuente en el sexo masculino. Predominó la obesidad grave y 8 de cada 10 niños presentaron 3 criterios del síndrome. La prevalencia de cada uno de los componentes fue del 33,3 % en la alteración del metabolismo de la glucosa, del 100 % en la obesidad central e hipertensión arterial, del 51,8 % en la hipertrigliceridemia y del 13 % en el colesterol de lipoproteínas de alta densidad (HDL-C bajo. Casi la mitad de los pacientes tenían microalbuminuria. El 31,5 % de los sujetos estudiados presentó insulinorresistencia y en el 29,5 % de los casos se encontró hígado graso no alcohólico. CONCLUSIONES.Uno de cada 3 hipertensos obesos tenía síndrome metabólico, por lo que se recomienda continuar investigando sobre este síndrome en la infancia y la adolescencia.

  8. RG domain wall for the general (su)-hat (2) coset models

    Energy Technology Data Exchange (ETDEWEB)

    Stanishkov, Marian [Institute for Nuclear Research and Nuclear Energy,Bulgarian Academy of Sciences, 1784 Sofia (Bulgaria)

    2016-08-16

    We consider a RG flow in a general (su)-hat (2) coset model induced by the least relevant field. This is done using two different approaches. We first compute the mixing coefficients of certain fields in the UV and IR theories using a conformal perturbation theory. The necessary structure constants are computed. The same coefficients can be calculated using the RG domain wall construction of Gaiotto. We compute the corresponding one-point functions and show that the two approaches give the same result in the leading order.

  9. Neuroprotective effects of ginsenoside Rg1 against oxygen-glucose deprivation in cultured hippocampal neurons.

    Science.gov (United States)

    He, Qing; Sun, Jianguo; Wang, Qin; Wang, Wei; He, Bin

    2014-03-01

    Ginsenoside Rg1 (Rg1) is believed to be one of the main active principles in ginseng, a traditional Chinese medicine extensively used to enhance stamina and deal with fatigue as well as physical stress. It has been reported that Rg1 performs multiple biological activities, including neuroprotective activity. In this study, we investigated the efficacy of ginsenoside Rg1 on ischemia-reperfusion injury in cultured hippocampal cells and also probed its possible mechanisms. To establish a model of oxygen-glucose deprivation (OGD) and reperfusion, cultured hippocampal neurons were exposed to OGD for 2.5 hours, followed by a 24-hour reoxygenation. Cultured hippocampal neurons were randomly divided into control group, model group (vehicle), and ginsenoside Rg1 treatment groups (5μM, 20μM, 60μM). At 24 hours post-OGD, the intracellular free calcium concentration was detected using Furo-3/AM-loaded hippocampal neurons deprived of oxygen and glucose. Neuronal nitric oxide synthase (nNOS) activity was measured by chemical colorimetry. Cell apoptosis was evaluated by Hoechst staining, and the neuron viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Excitotoxic neuronal injury of OGD was demonstrated by the increase of intracellular free calcium concentrations and elevated nNOS activity in the model group compared with the control group. The intracellular free calcium concentrations and the nNOS activity in the groups receiving intermediate and high dose of ginsenoside Rg1 were significantly lower than those of the control group (p cell viability loss (p cell apoptosis induced by OGD. Ginsenoside Rg1 has neuroprotective effect on ischemia-reperfusion injury in cultured hippocampal cells mediated by blocking calcium over-influx into neuronal cells and decreasing the nNOS activity after OGD exposure. We infer that ginsenoside Rg1 may serve as a potential therapeutic agent for cerebral ischemia injury. Copyright © 2014

  10. Promoción de la salud: clínica de diabetes y síndrome metabólico de la FES Iztacala UNAM

    OpenAIRE

    Álvarez-Gasca, M.A.; Hernández-Pozo, M.R.; Alan-Guzmán, S.

    2017-01-01

    Introducción: Las enfermedades relacionadas con síndrome metabólico representan un problema de salud pública en México y la Universidad Nacional Autónoma de México como parte de sus actividades asume el compromiso social de inferir en programas de salud. Así la clínica de diabetes y síndrome metabólico de la FESIztacala-UNAM, tiene como Objetivo: Promover la salud mediante medidas de prevención y tratamiento oportuno para reducir la incidencia de sobrepeso, obesidad, diabetes y síndrome metab...

  11. Síndrome metabólica: importância de seu reconhecimento

    Directory of Open Access Journals (Sweden)

    Sthefano Atique Gabriel

    2007-06-01

    Full Text Available A Síndrome Metabólica caracteriza-se por um agrupamento de fatores de risco para doenças cardiovasculares e diabetes, geralmente ligados à resistência à insulina, à obesidade e à obesidade central, tais como, hipertensão arterial sistêmica, obesidade abdominal, tolerância à glicose prejudicada, hipertrigliceridemia e baixas concentrações sanguíneas de HDL-colesterol, além dos estados pró-trombótico e pró-inflamatório observados. Uma alimentação adequada, entretanto, associada a modificações no estilo de vida, tais como prática regular de atividade física e abandono do tabagismo, contribui para um melhor controle da doença, prevenindo suas complicações e aumentando a qualidade de vida.

  12. Modelización del control metabólico: cursos basados en el autoaprendizaje

    OpenAIRE

    Aguilera Mochón, Juan Antonio; Departamento de Bioquímica y Biología molecular, Universidad de Alcalá de Henares, Madrid

    2013-01-01

    Introducción. La necesidad de un análisis cuantitativo de las rutas bioquímicas y de su control llevó al desarrollo del Análisis del Control Metabólico (MCA, por las siglas en inglés). Por otro lado, dada la extraordinaria complejidad de los procesos biológicos a nivel molecular, su comprensión es imposible sin el auxilio de procedimientos de modelización. En la actualidad, los alumnos de Bioquímica se quedan lejos de la comprensión o visualización del funcionamiento real –siquiera en término...

  13. Metab2MeSH: annotating compounds with medical subject headings.

    Science.gov (United States)

    Sartor, Maureen A; Ade, Alex; Wright, Zach; States, David; Omenn, Gilbert S; Athey, Brian; Karnovsky, Alla

    2012-05-15

    Progress in high-throughput genomic technologies has led to the development of a variety of resources that link genes to functional information contained in the biomedical literature. However, tools attempting to link small molecules to normal and diseased physiology and published data relevant to biologists and clinical investigators, are still lacking. With metabolomics rapidly emerging as a new omics field, the task of annotating small molecule metabolites becomes highly relevant. Our tool Metab2MeSH uses a statistical approach to reliably and automatically annotate compounds with concepts defined in Medical Subject Headings, and the National Library of Medicine's controlled vocabulary for biomedical concepts. These annotations provide links from compounds to biomedical literature and complement existing resources such as PubChem and the Human Metabolome Database.

  14. Comportamiento endocrino-metabólico de las fracturas de tercio medio facial

    Directory of Open Access Journals (Sweden)

    Berta Beauballet Fernández

    1997-12-01

    Full Text Available Se valoran los resultados del comportamiento endocrino-metabólico en 40 pacientes con fracturas del tercio medio facial: 10 nasales, 20 maxilomalares, 2 Lefort I, 4 Lefort II y 4 Lefort III. Según el sexo, 34 pacientes correspondieron al sexo masculino y 6 al femenino. Cuantificamos en orina (muestras de 24 horas: cloruro, sodio, potasio, urea y creatinina; en el ionograma en sangre: cloro, sodio, y potasio; en la química sanguínea: glucosa, clacio, fósforo, urea y creatinina y hemograma con diferencial. Estas muestras se cuantificaron al llegar el paciente, a las 72 horas y a las 24 horas del posoperatorio inmediato. En todos los pacientes estudiados se mantuvo la estabilidad endocrino-metabólica, con excepción de aquéllos portadores de Lefort I, II y III, los cuales en la última muestra tomada presentaron alteraciones del sodio y potasio en el ionograma de orina y sangre.The results of the endocrine and metabolic behaviour in 40 patient with fractures of the medial facial third: 10 nasal, 20 maxillomalar, 2 Lefort I, 4 Lefort II, and 4 Lefort III, are evaluated. 34 patients were males and 6 females. Chloride, sodium, potassium, urea, and creatinine were cuantified in urine (24-hour samples; chlorine, sodium, and potassium in blood ionogram; glucose, calcium, phosphorus, urea, creatinine, and differential blood count in blood chemistry. These samples were taken 72 hours and 24 hours after the immediate postoperative. All the patients studied mantained the endocrine and metabolic stability except those with Leford I, II and III, who in the last sample taken presented alterations of sodium and potassium in the urine and blood ionogram.

  15. Intracellular Trafficking Modulation by Ginsenoside Rg3 Inhibits Brucella abortus Uptake and Intracellular Survival within RAW 264.7 Cells.

    Science.gov (United States)

    Huy, Tran Xuan Ngoc; Reyes, Alisha Wehdnesday Bernardo; Hop, Huynh Tan; Arayan, Lauren Togonon; Min, WonGi; Lee, Hu Jang; Rhee, Man Hee; Chang, Hong Hee; Kim, Suk

    2017-03-28

    Ginsenoside Rg3, a saponin extracted from ginseng, has various pharmacological and biological activities; however, its effects against Brucella infection are still unclear. Herein, the inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as immunoblotting and FACS for detecting MAPK signaling proteins and F-actin polymerization, respectively. The internalization, intracellular growth, and adherence of Brucella abortus in Rg3-treated RAW 264.7 cells were significantly decreased compared with the Rg3-untreated control. Furthermore, an apparent reduction of F-actin content and intensity of F-actin fluorescence in Rg3-treated cells was observed compared with B. abortus -infected cells without treatment by flow cytometry analysis and confocal microscopy, respectively. In addition, treating cells with Rg3 decreased the phosphorylation of MAPK signaling proteins such as ERK 1/2 and p38 compared with untreated cells. Moreover, the colocalization of B. abortus -containing phagosomes with LAMP-1 was markedly increased in Rg3-treated cells. These findings suggest that ginsenoside Rg3 inhibits B. abortus infection in mammalian cells and can be used as an alternative approach in the treatment of brucellosis.

  16. Ginsenosides Rb1 and Rg1 Stimulate Melanogenesis in Human Epidermal Melanocytes via PKA/CREB/MITF Signaling

    Directory of Open Access Journals (Sweden)

    Mao Lin

    2014-01-01

    Full Text Available Reduced or defective melanin skin pigmentation may cause many hypopigmentation disorders and increase the risk of damage to the skin triggered by UV irradiation. Ginsenosides Rb1 and Rg1 have many molecular targets including the cAMP-response element-binding protein (CREB, which is involved in melanogenesis. This study aimed to investigate the effects of ginsenosides Rb1 and Rg1 on melanogenesis in human melanocytes and their related mechanisms. The effects of Rb1 and Rg1 on cell viability, tyrosinase activity, cellular melanin content and protein levels of tyrosinase, microphthalmia-associated transcription factor (MITF, and activation of CREB in melanocytes were assessed. Results showed that Rb1 or Rg1 significantly increased cellular melanin content and tyrosinase activity in a dose-dependent manner. By contrast, the cell viability of melanocytes remained unchanged. After exposure to Rb1 or Rg1, the protein levels of tyrosinase, MITF, and phosphorylated CREB were significantly increased. Furthermore, pretreatment with the selective PKA inhibitor H-89 significantly blocked the Rb1- or Rg1-induced increase of melanin content. These findings indicated that Rb1 and Rg1 increased melanogenesis and tyrosinase activity in human melanocytes, which was associated with activation of PKA/CREB/MITF signaling. The effects and mechanisms of Rb1 or Rg1 on skin pigmentation deserve further study.

  17. Remarks on the Landau-Ginzburg potential and RG-flow for SU(2)-coset models

    International Nuclear Information System (INIS)

    Marzban, C.

    1989-09-01

    The existence of a Landau-Ginzburg (LG)-field for the SU(2)-coset models is motivated and conjectured. The general form of the LG potential for the A-series is found, and the RG-flow pattern suggested by this is shown to agree with that found by other authors, thereby further supporting the conjecture. (author). 17 refs

  18. Kolm küsimust Rait Pärgile / Rait Pärg ; interv. Katrin Nielsen

    Index Scriptorium Estoniae

    Pärg, Rait

    2001-01-01

    Skulptor Rait Pärg Pärnu skulptuurikaardi ja selle põhjal skulptuurikontseptsiooni koostamisest (osalevad ka Omar Volmer ja Mark Soosaar), 2.-11. juunini korraldatavatest skulptuuripäevadest "Pärnu - positiivse märgiga linn"

  19. Effect of enzyme on extraction of ginsenoside Rb1 and Rg3 from Panax notoginseng roots

    Science.gov (United States)

    Phuong, Nguyen Tran Xuan; Thy, Lu Thi Mong; Khang, Nguyen Luu Vinh; My, Huynh Thi Kieu; Tam, Nguyen Le Phuong; Hieu, Nguyen Huu

    2018-04-01

    Panax notoginseng is distributed throughout the north and northwest of Vietnam, especially Ha Giang, Lao Cai, and Cao Bang provinces. The root of this plant contains ginsenosides (Rb1, Rb2, Rd, Rg3), flavonoids, polyacetylene, polysaccharides, amino acids, fatty acids, and peptides. In this study, the ratios of enzyme (Viscozyme, Termamyl, Cellulase), solvent of components, and time extraction were investigated. The results showed that the highest contents of Rb1 and Rg3 were achieved in the sample extracted with the ratio of enzymes V:C:T = 1:0:0, ethanol:water (60:40, v/v) as extracting solvent in 45 minutes. Then, conditions of high performance liquid chromatography with diode array detector method to determine the content of ginsenosides Rb1 and Rg3 in the roots of Panax notoginseng were studied, including wavelength, mobile phase, and flow rate. The separation was subjected on a reversed-phase C18 column using acetonitrile (A) and water (B) as mobile phase. The gradient elution was set as follow: 0-10 min, 15-25% A; 10-20 min, 25-30% A; 20-40 min, 30-60% A; 40-60 min, 60-80% A; and 60-65 min back to 15% A before the next injection, at a flow rate of 0.5 mL/min, and the wavelength was set at 202 nm. The linear range was from 298.59 to 696.72 µg/mL for Rb1 and from 8.19 to 19.10 µg/L for Rg3. The limits of detection for Rb1 and Rg3 obtained were 0.31 µg/mL and 0.33 µg/mL, respectively. The limits of quantification were 0.95 µg/mL and 1.01 µg/mL for Rb1 and Rg3, respectively. Consequently, the high performance liquid chromatography demonstrated the highly sensitive and accurate method for determination of Rb1 and Rg3 in Panax notoginseng.

  20. Efeitos da estimulação elétrica neuromuscular sobre o membro posterior imobilizado de ratos durante 15 dias: análises metabólicas e morfométricas Effects of neuromuscular electrical stimulation on rat hind limbs immobilized for 15 days: metabolic and morphometric analyses

    Directory of Open Access Journals (Sweden)

    JLQ Durigan

    2006-09-01

    Full Text Available OBJETIVO: Avaliar o efeito da estimulação elétrica (EE sobre o perfil metabólico e morfométrico dos músculos do membro posterior de ratos submetidos à imobilização durante 15 dias. MÉTODO: Ratos Wistar foram divididos em 3 grupos (n=5: controle, imobilizado por 15 dias e imobilizado associado à EE por 15 dias. Foram avaliados: reserva de glicogênio (RG dos músculos sóleo (S, extensor longo dos dedos (ELD, gastrocnêmio branco (GB, gastrocnêmio vermelho (GV e tibial anterior (TA, além do peso do sóleo, área das fibras e tecido conjuntivo do S. A análise estatística foi feita pelos testes ANOVA e Kruskal-Wallis (pOBJECTIVE: To evaluate the effect of electrical stimulation on the metabolic and morphometric profile of rat hind limb muscles subjected to immobilization for 15 days. METHOD: Wistar rats were divided into three groups (n=5: control; immobilized for 15 days; and immobilized for 15 days with electrical stimulation. The glycogen reserves of the soleus, extensor digitorum longus (EDL, white gastrocnemius (WG, red gastrocnemius (RG and tibialis anterior (TA muscles were evaluated, along with the weight, fibrous area and conjunctive tissue of the soleus. The statistical analysis was performed using the Anova and Kruskal-Wallis tests (p<0.05. RESULTS: Immobilization promoted significant alterations (p<0.05, such as: reductions in the glycogen reserves (soleus: 44.73%, WG: 47.82%, RG: 46.34%, EDL: 41.66%, TA: 48.38% and in the weight (7.2% and fibrous area (35% of the soleus, and also increased connective tissue density (160%. Electrical stimulation promoted a significant increase (p<0.05 in the glycogen reserves of all the immobilized muscles: (soleus: 90.47%, WG: 62.5%, RG: 95.45%, EDL: 76.19%, TA: 56.25% and in the weight (20.94% and fibrous area (19.65% of the soleus, and also promoted a significant reduction (15.38%, p<0.05 in connective tissue density. CONCLUSION: Electrical stimulation minimized the reduction in

  1. Immunogenic Cell Death Induced by Ginsenoside Rg3: Significance in Dendritic Cell-based Anti-tumor Immunotherapy.

    Science.gov (United States)

    Son, Keum-Joo; Choi, Ki Ryung; Lee, Seog Jae; Lee, Hyunah

    2016-02-01

    Cancer is one of the leading causes of morbidity and mortality worldwide; therefore there is a need to discover new therapeutic modules with improved efficacy and safety. Immune-(cell) therapy is a promising therapeutic strategy for the treatment of intractable cancers. The effectiveness of certain chemotherapeutics in inducing immunogenic tumor cell death thus promoting cancer eradication has been reported. Ginsenoside Rg3 is a ginseng saponin that has antitumor and immunomodulatory activity. In this study, we treated tumor cells with Rg3 to verify the significance of inducing immunogenic tumor cell death in antitumor therapy, especially in DC-based immunotherapy. Rg3 killed the both immunogenic (B16F10 melanoma cells) and non-immunogenic (LLC: Lewis Lung Carcinoma cells) tumor cells by inducing apoptosis. Surface expression of immunogenic death markers including calreticulin and heat shock proteins and the transcription of relevant genes were increased in the Rg3-dying tumor. Increased calreticulin expression was directly related to the uptake of dying tumor cells by dendritic cells (DCs): the proportion of CRT(+) CD11c(+) cells was increased in the Rg3-treated group. Interestingly, tumor cells dying by immunogenic cell death secreted IFN-γ, an effector molecule for antitumor activity in T cells. Along with the Rg3-induced suppression of pro-angiogenic (TNF-α) and immunosuppressive cytokine (TGF-β) secretion, IFN-γ production from the Rg3-treated tumor cells may also indicate Rg3 as an effective anticancer immunotherapeutic strategy. The data clearly suggests that Rg3-induced immunogenic tumor cell death due its cytotoxic effect and its ability to induce DC function. This indicates that Rg3 may be an effective immunotherapeutic strategy.

  2. Desigualdades sociales en la mortalidad de enfermedades por causas externas, tumores, enfermedades metabólicas en el departamento de Caldas 2010-2013

    OpenAIRE

    Marín Ocampo, Angela María

    2017-01-01

    Objetivo. Determinar las desigualdades sociales en la mortalidad de enfermedades por causas externas, enfermedades metabólicas y tumores en los municipios del departamento de Caldas en el período 2010-2013.

  3. Kamu personelinde algılanan örgütsel desteğin örgütsel bağlılık ve örgütsel özdeşleşme düzeyleri ile ilişkisini belirlemeye yönelik bir araştırma

    OpenAIRE

    Barutcu, Kürşad

    2015-01-01

    Algılanan örgütsel destek (AÖD) bireylerin örgüte olan katkılarının ne derece önemsendiği, refahlarının ne derece dikkate alındığı ve üyesi oldukları örgütlerinin kedilerini ne derece destekleyip güvende hissetmelerini sağladıklarına olan inançlarıdır. Bireylerin örgütsel destek algılarının olumlu olduğu ortamlarda bireylerin karşılık olarak bir takım olumlu tepkilerde bulunacakları literatürde yer alan birçok araştırmada ifade edilmiştir. Bu kapsamda değerlendirildiğinde örgüt...

  4. Estudio y caracterización de la respuesta fisiologíca y metabólica del niño obeso en reposo y esfuerzo

    OpenAIRE

    Guixeres Provinciale, Jaime

    2014-01-01

    Antecedentes: Existen hasta la fecha bastantes trabajos de la respuesta metabólica y cardiovascular en adultos y algunos menos en niños pero muy pocos se han centrado en investigar acerca de las alteraciones cardiorespiratorias y metabólicas que implica la obesidad infantil. Una auténtica epidemia del siglo XXI. Se hace necesario instaurar nuevos marcadores para adaptar los tratamientos en este campo Objetivo: Mejorar y avanzar en el conocimiento que permita proponer nuevos...

  5. Tratamiento de la hipertensión arterial en el paciente con síndrome metabólico

    Directory of Open Access Journals (Sweden)

    Patricio López-Jaramillo, MD., PhD.

    2010-01-01

    Full Text Available El síndrome metabólico afecta alrededor de 25% a 45% de la población colombiana de acuerdo con los criterios diagnósticos propuestos por la Federación Internacional de Diabetes, los cuales, en nuestra población, aparecen como los más útiles. Bien se sabe que el síndrome metabólico se asocia con un incremento tres a seis veces en el riesgo de desarrollar diabetes y nuevos casos de hipertensión. En Colombia, un estudio de cohorte realizado en pacientes que sufrieron infarto agudo del miocardio, demostró que la resistencia a la insulina fue el principal factor que predijo muerte cardiovascular o presencia de nuevos eventos cardio-cerebro-vasculares. Además, la presencia de síndrome metabólico se asocia con más frecuencia a daño subclínico de órgano blanco. El principal tratamiento en sujetos con síndrome metabólico es la reducción del peso corporal a través de la implementación de una dieta baja en calorías y aumento del ejercicio físico. Así, los pacientes con este síndrome necesitan la administración adicional de medicamentos antihipertensivos, antidiabéticos orales o fármacos hipolipemiantes cuando existe franca hipertensión, diabetes o dislipidemia. En vista de que el riesgo cardiovascular es alto en pacientes hipertensos con síndrome metabólico, es necesario hacer un riguroso control de la presión arterial, manteniendo los niveles siempre por debajo de 130/85 mm Hg. A no ser que existan indicaciones específicas, en los pacientes con síndrome metabólico se debe evitar el uso de beta-bloqueadores, ya que son bien conocidos sus efectos adversos en el aumento de peso, en la incidencia de nuevos casos de diabetes, en la resistencia a la insulina y en el perfil lipídico. Los diuréticos tiazídicos presentan efectos diabetogénicos y otras acciones dismetabólicas, especialmente a dosis altas, por lo que no deben utilizarse en pacientes con síndrome metabólico. Por lo tanto, los fármacos recomendados como

  6. O potencial da rotulação metabólica de 15N para a pesquisa de esquizofrenia

    Directory of Open Access Journals (Sweden)

    Michaela D. Filiou

    2013-01-01

    Full Text Available Pesquisas em psiquiatria ainda necessitam de estudos não dirigidos por hipóteses para revelar fundamentos neurobiológicos e biomarcadores moleculares para distúrbios psiquiátricos. Metodologias proteômicas disponibilizam uma série de ferramentas para esses fins. Apresentamos o princípio de rotulação metabólica utilizando 15N para proteômica quantitativa e suas aplicações em modelos animais de fenótipos psiquiátricos com um foco particular em esquizofrenia. Exploramos o potencial de rotulação metabólica por 15N em diferentes tipos de experimentos, bem como suas considerações metodológicas.

  7. Hiperidricidade: uma desordem metabólica Hyperhydricity: a metabolic disorder

    Directory of Open Access Journals (Sweden)

    Andréa Guimarães Vieira de Vasconcelos

    2012-05-01

    Full Text Available A hiperidricidade, anteriormente chamada vitrificação, é considerada uma desordem fisiológica, bioquímica e morfológica decorrente do acúmulo anormal de água no interior das células e tecidos. As plantas cultivadas in vitro estão, indubitavelmente, sob contínua condição de estresse, os quais resultam em alterações metabólicas características do estresse oxidativo. Anatomicamente, plantas ou brotos afetados frequentemente apresentam-se inchados, com coloração verde claro, folhas translúcidas e com aparência de vidro, baixa relação número de células/área celular e hipolignificação. Alterações fisiológicas que ocorrem nas principais vias metabólicas, incluindo fotossíntese, respiração e transpiração, resultam em redução de eficiência dessas vias metabólicas. Os distúrbios morfológicos, fisiológicos e bioquímicos são desencadeados por fatores físicos, relacionados ao ambiente dos recipientes de cultivo e consistência do meio de cultura ou por fatores químicos como os componentes do meio de cultura, em especial dos reguladores de crescimento em altas concentrações. A hiperidricidade ocorre em vários níveis de severidade, chegando a resultar na perda irreversível da capacidade morfogênica e o estabelecimento de um estado neoplásico das células, no entanto, na maioria dos casos, a hiperidricidade é considerada reversível. Esta revisão foca o conhecimento atual sobre o fenômeno da hiperidricidade abordando aspectos morfológicos, fisiológicos, bioquímicos e a reversibilidade do processo.The hyperhydricity, formerly called vitrification, is considered a physiological, biochemistry and morfologic disorder due to abnormal accumulation of water inside the cells and tissues. Plants grown in vitro are undoubtedly under continuous stress condition which results in metabolic changes characteristic of oxidative stress. Anatomically plants or shoots affected often become swollen, with pale green

  8. Suplementos nutricionales como modificadores del riesgo cardiovascular en componentes del síndrome metabólico en adultos

    Directory of Open Access Journals (Sweden)

    Stefano Tassinari

    2017-05-01

    Conclusión: El uso de suplementos nutricionales con ácidos grasos de cadena larga y antioxidante podría tener efectos benéficos en la disminución de riesgo cardiovascular en pacientes con diagnóstico de síndrome metabólico o alguno de sus componentes. El uso de suplementos con otros componentes debe estudiarse a mayor profundidad para efectuar recomendaciones.

  9. Ginsenoside Rg1 enhances lymphatic transport of intrapulmonary silica via VEGF-C/VEGFR-3 signaling in silicotic rats.

    Science.gov (United States)

    Yu, Jie; Mao, Lijun; Guan, Li; Zhang, Yanlin; Zhao, Jinyuan

    2016-03-25

    Ginsenoside Rg1, extracted mainly from Panax ginseng, has been shown to exert strong pro-angiogenic activities in vivo. But it is unclear whether ginsenoside Rg1 could promote lung lymphangiogenesis to improve lymphatic transport of intrapulmonary silica in silicotic rats. Here we investigated the effect of ginsenoside Rg1 on lymphatic transport of silica during experimental silicosis, and found that ginsenoside Rg1 treatment significantly raised the silicon content in tracheobronchial lymph nodes and serum to reduce the silicon level in lung interstitium, meanwhile increased pulmonary lymphatic vessel density by enhancing the protein and mRNA expressions of vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3). The stimulative effect of ginsenoside Rg1 on lymphatic transport of silica was actively correlated with its pro-lymphangiogenic identity. And VEGFR-3 inhibitor SAR131675 blocked these above effects of ginsenoside Rg1. These findings suggest that ginsenoside Rg1 exhibits good protective effect against lung burden of silica during experimental silicosis through improving lymphatic transport of intrapulmonary silica, which is potentially associated with the activation of VEGF-C/VEGFR-3 signaling pathway. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Chronic treatment with ginsenoside Rg1 promotes memory and hippocampal long-term potentiation in middle-aged mice.

    Science.gov (United States)

    Zhu, G; Wang, Y; Li, J; Wang, J

    2015-04-30

    Ginseng serves as a potential candidate for the treatment of aging-related memory decline or memory loss. However, the related mechanism is not fully understood. In this study, we applied an intraperitoneal injection of ginsenoside Rg1, an active compound from ginseng in middle-aged mice and detected memory improvement and the underlying mechanisms. Our results showed that a period of 30-day administration of ginsenoside Rg1 enhanced long-term memory in the middle-aged animals. Consistent with the memory improvement, ginsenoside Rg1 administration facilitated weak theta-burst stimulation (TBS)-induced long-term potentiation (LTP) in acute hippocampal slices from middle-aged animals. Ginsenoside Rg1 administration increased the dendritic apical spine numbers and area in the CA1 region. In addition, ginsenoside Rg1 administration up-regulated the expression of hippocampal p-AKT, brain-derived neurotrophic factor (BDNF), proBDNF and glutamate receptor 1 (GluR1), but not p-ERK. Interestingly, the phosphatase and tensin homolog deleted on chromosome ten (PTEN) inhibitor (bpV) mimicked the ginsenoside Rg1 effects, including increasing p-AKT expression, promoting hippocampal basal synaptic transmission, LTP and memory. Taken together, our data suggest that ginsenoside Rg1 treatment improves memory in middle-aged mice possibly through regulating the PI3K/AKT pathway, altering apical spines and facilitating hippocampal LTP. Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

  11. Physiological Expression of AMPKγ2RG Mutation Causes Wolff-Parkinson-White Syndrome and Induces Kidney Injury in Mice.

    Science.gov (United States)

    Yang, Xiaodong; Mudgett, John; Bou-About, Ghina; Champy, Marie-France; Jacobs, Hugues; Monassier, Laurent; Pavlovic, Guillaume; Sorg, Tania; Herault, Yann; Petit-Demoulière, Benoit; Lu, Ku; Feng, Wen; Wang, Hongwu; Ma, Li-Jun; Askew, Roger; Erion, Mark D; Kelley, David E; Myers, Robert W; Li, Cai; Guan, Hong-Ping

    2016-11-04

    Mutations of the AMP-activated kinase gamma 2 subunit (AMPKγ2), N488I (AMPKγ2 NI ) and R531G (AMPKγ2 RG ), are associated with Wolff-Parkinson-White (WPW) syndrome, a cardiac disorder characterized by ventricular pre-excitation in humans. Cardiac-specific transgenic overexpression of human AMPKγ2 NI or AMPKγ2 RG leads to constitutive AMPK activation and the WPW phenotype in mice. However, overexpression of these mutant proteins also caused profound, non-physiological increase in cardiac glycogen, which might abnormally alter the true phenotype. To investigate whether physiological levels of AMPKγ2 NI or AMPKγ2 RG mutation cause WPW syndrome and metabolic changes in other organs, we generated two knock-in mouse lines on the C57BL/6N background harboring mutations of human AMPKγ2 NI and AMPKγ2 RG , respectively. Similar to the reported phenotypes of mice overexpressing AMPKγ2 NI or AMPKγ2 RG in the heart, both lines developed WPW syndrome and cardiac hypertrophy; however, these effects were independent of cardiac glycogen accumulation. Compared with AMPKγ2 WT mice, AMPKγ2 NI and AMPKγ2 RG mice exhibited reduced body weight, fat mass, and liver steatosis when fed with a high fat diet (HFD). Surprisingly, AMPKγ2 RG but not AMPKγ2 NI mice fed with an HFD exhibited severe kidney injury characterized by glycogen accumulation, inflammation, apoptosis, cyst formation, and impaired renal function. These results demonstrate that expression of AMPKγ2 NI and AMPKγ2 RG mutations at physiological levels can induce beneficial metabolic effects but that this is accompanied by WPW syndrome. Our data also reveal an unexpected effect of AMPKγ2 RG in the kidney, linking lifelong constitutive activation of AMPK to a potential risk for kidney dysfunction in the context of an HFD. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  12. Physiological Expression of AMPKγ2RG Mutation Causes Wolff-Parkinson-White Syndrome and Induces Kidney Injury in Mice*

    Science.gov (United States)

    Yang, Xiaodong; Mudgett, John; Bou-About, Ghina; Champy, Marie-France; Jacobs, Hugues; Monassier, Laurent; Pavlovic, Guillaume; Sorg, Tania; Herault, Yann; Petit-Demoulière, Benoit; Lu, Ku; Feng, Wen; Wang, Hongwu; Ma, Li-Jun; Askew, Roger; Erion, Mark D.; Kelley, David E.; Myers, Robert W.; Li, Cai

    2016-01-01

    Mutations of the AMP-activated kinase gamma 2 subunit (AMPKγ2), N488I (AMPKγ2NI) and R531G (AMPKγ2RG), are associated with Wolff-Parkinson-White (WPW) syndrome, a cardiac disorder characterized by ventricular pre-excitation in humans. Cardiac-specific transgenic overexpression of human AMPKγ2NI or AMPKγ2RG leads to constitutive AMPK activation and the WPW phenotype in mice. However, overexpression of these mutant proteins also caused profound, non-physiological increase in cardiac glycogen, which might abnormally alter the true phenotype. To investigate whether physiological levels of AMPKγ2NI or AMPKγ2RG mutation cause WPW syndrome and metabolic changes in other organs, we generated two knock-in mouse lines on the C57BL/6N background harboring mutations of human AMPKγ2NI and AMPKγ2RG, respectively. Similar to the reported phenotypes of mice overexpressing AMPKγ2NI or AMPKγ2RG in the heart, both lines developed WPW syndrome and cardiac hypertrophy; however, these effects were independent of cardiac glycogen accumulation. Compared with AMPKγ2WT mice, AMPKγ2NI and AMPKγ2RG mice exhibited reduced body weight, fat mass, and liver steatosis when fed with a high fat diet (HFD). Surprisingly, AMPKγ2RG but not AMPKγ2NI mice fed with an HFD exhibited severe kidney injury characterized by glycogen accumulation, inflammation, apoptosis, cyst formation, and impaired renal function. These results demonstrate that expression of AMPKγ2NI and AMPKγ2RG mutations at physiological levels can induce beneficial metabolic effects but that this is accompanied by WPW syndrome. Our data also reveal an unexpected effect of AMPKγ2RG in the kidney, linking lifelong constitutive activation of AMPK to a potential risk for kidney dysfunction in the context of an HFD. PMID:27621313

  13. Effect of ginsenoside Rg3 on tyrosine hydroxylase and related mechanisms in the forced swimming-induced fatigue rats.

    Science.gov (United States)

    Xu, Yuxia; Zhang, Peng; Wang, Chu; Shan, Ye; Wang, Dandan; Qian, Fenglei; Sun, Mengwei; Zhu, Cuiqing

    2013-10-28

    Ginsenoside Rg3 has shown multiple pharmacological activities and been considered as one of the most promising approaches for fatigue treatment. However, little is known about the cellular and molecular mechanisms of Rg3 on anti-fatigue and the effect of Rg3 on dopaminergic system has not been reported yet. The major aim of this study is to investigate the effect of Rg3 on TH expression and the related biochemical parameters, such as PKAα, ERK1/2, Akt and α-synuclein in brain of fatigue rats. Weight-loaded forced swimming was performed to establish an animal model of fatigue. Rg3 (10mg/kg, 50mg/kg and 100mg/kg) was intragastrically administrated before swimming. The effect of Rg3 on the expression and phosphorylation of TH and TH-related proteins in fatigue rats or in SH-SY5Y cells was assessed with western blotting. HPLC was used to examine the level of DA and DOPAC in the fatigue rats tissues. TH and phosphorylated TH were decreased in different brain regions of which ventral midbrain were less affected in weight-loaded forced swimming rats. Pretreatment with Rg3 significantly suppressed fatigue-induced decrease expression of TH and TH phosphorylation. Also treatment with Rg3 reversed the decrease expression of PKAα as well as the phosphorylation of ERK1/2 and Akt which were induced by weight-loaded forced swimming. Moreover, weight-loaded swimming could induce the increase expression of α-synuclein in hippocampus and midbrain, while suppressed α-synuclein expression in striatum and prefrontal cortex. Furthermore, Rg3 could induce the increase of TH expression and phosphorylation which was accompanied with elevated expression and phosphorylation of related kinase proteins in vitro, while the inhibitors of kinase proteins could suppress these effects of Rg3. In addition, HPLC results showed that Rg3 could reverse the weight-loaded swimming-induced increase of DOPAC/DA ratio. Our data suggest that fatigue can induce the decrease of DA which might partially

  14. Índice cronotrópico-metabólico na doença de Chagas

    Directory of Open Access Journals (Sweden)

    Rocha Ana Luiza Lunardi

    2005-01-01

    Full Text Available A insuficiência cronotrópica constitui achado comum entre os pacientes chagásicos. Novas metodologias estão sendo empregadas na avaliação da resposta cronotrópica em vários grupos de pacientes. O índice cronotrópico-metabólico, um desses novos métodos, quantifica a relação entre o aumento da freqüência cardíaca e o consumo máximo de oxigênio (VO2 max durante o teste ergométrico. A resposta normal é linear, com índice em torno de 1,0. Objetivamos avaliar a resposta cronotrópica e em indivíduos saudáveis e pacientes chagásicos com e sem disfunção ventricular esquerda, utilizando-se do índice cronotrópico-metabólico. Foram avaliados 171 pacientes com doença de Chagas sem doenças associadas e 24 controles submetidos a protocolo clínico e ao teste ergométrico máximo. Os chagásicos foram divididos em dois grupos: Ch1= pacientes com fração de ejeção (FE > 39% e Ch 2= FE<40%. A análise e o cálculo do índice cronotrópico-metabólico foram feitos pelo método de Wilkoff. Os pacientes chagásicos apresentaram maior idade e maior prevalência de bloqueio completo de ramo direito, assim como menor VO2 max ao teste ergométrico. Ambos os grupos de chagásicos apresentaram menor inclinação do índice cronotrópico-metabólico (Ch1: 0,91±0,10, Ch2: 0,89±0,08 do que os controles (1,0±0,12, p< 0,001. Pacientes com doença de Chagas com e sem disfunção ventricular esquerda podem apresentar resposta cronotrópica deprimida, manifesta por menor inclinação do índice cronotrópico-metabólico.

  15. Impact of isomalathion on malathion cytotoxicity and genotoxicity in human HepaRG cells.

    OpenAIRE

    Josse , Rozenn; Sharanek , Ahmad; Savary , Camille C; Guillouzo , André

    2014-01-01

    International audience; Isomalathion is a major impurity of technical grade malathion, one of the most abundantly applied insecticides; however little is known about its hepatotoxicity. In the present study, cytotoxicity and genotoxicity of malathion and isomalathion either individually or in combination, were assessed using the metabolically competent human liver HepaRG cell line. Isomalathion reduced cell viability starting at a 100 μM concentration after a 24h exposure. It also significant...

  16. Laboratory Measurement of the Brighter-fatter Effect in an H2RG Infrared Detector

    OpenAIRE

    Plazas, A. A.; Shapiro, C.; Smith, R.; Huff, E.; Rhodes, J.

    2018-01-01

    The "brighter-fatter" (BF) effect is a phenomenon (originally discovered in charge coupled devices) in which the size of the detector point spread function (PSF) increases with brightness. We present, for the first time, laboratory measurements demonstrating the existence of the effect in a Hawaii-2RG HgCdTe near infrared (NIR) detector. We use the Precision Projector Laboratory, a JPL facility for emulating astronomical observations with UV/VIS/NIR detectors, to project about 17,000 point so...

  17. N=1 Deformations and RG flows of N=2 SCFTs, part II: non-principal deformations

    Energy Technology Data Exchange (ETDEWEB)

    Agarwal, Prarit [Department of Physics and Astronomy & Center for Theoretical Physics,Seoul National University,Seoul 151-747 (Korea, Republic of); Maruyoshi, Kazunobu [Faculty of Science and Technology, Seikei University,3-3-1 Kichijoji-Kitamachi, Musashino-shi, Tokyo, 180-8633 (Japan); Song, Jaewon [Department of Physics, University of California,San Diego, La Jolla, CA 92093 (United States)

    2016-12-20

    We continue to investigate the N=1 deformations of four-dimensional N=2 superconformal field theories (SCFTs) labeled by a nilpotent element of the flavor symmetry http://arxiv.org/abs/1607.04281. This triggers a renormalization group (RG) flow to an N=1 SCFT. We systematically analyze all possible deformations of this type for certain classes of N=2 SCFTs: conformal SQCDs, generalized Argyres-Douglas theories and the E{sub 6} SCFT. We find a number of examples where the amount of supersymmetry gets enhanced to N=2 at the end point of the RG flow. Most notably, we find that the SU(N) and Sp(N) conformal SQCDs can be deformed to flow to the Argyres-Douglas (AD) theories of type (A{sub 1},D{sub 2N−1}) and (A{sub 1},D{sub 2N}) respectively. This RG flow therefore allows us to compute the full superconformal index of the (A{sub 1},D{sub N}) class of AD theories. Moreover, we find an infrared duality between N=1 theories where the fixed point is described by an N=2 AD theory. We observe that the classes of examples that exhibit supersymmetry enhancement saturate certain bounds for the central charges implied by the associated two-dimensional chiral algebra.

  18. Evaluating vacquinol-1 in rats carrying glioblastoma models RG2 and NS1.

    Science.gov (United States)

    Ahlstedt, Jonatan; Förnvik, Karolina; Zolfaghari, Shaian; Kwak, Dongoh; Hammarström, Lars G J; Ernfors, Patrik; Salford, Leif G; Redebrandt, Henrietta Nittby

    2018-02-02

    Glioblastoma multiforme (GBM) is the most common malignant primary brain tumor, and available experimental and routine therapies result in limited survival benefits. A vulnerability of GBM cells to catastrophic vacuolization and cell death, a process termed methuosis, induced by Vacquinol-1 (VQ-1) has been described earlier. In the present study, we investigate the efficacy of VQ-1 treatment in two syngeneic rat GBM models, RG2 and NS1. VQ-1 treatment affected growth of both RG2 and NS1 cells in vitro . Intracranially, significant reduction in RG2 tumor size was observed, although no effect was seen on overall survival. No survival advantage or effect on tumor size was seen in animals carrying the NS1 models compared to untreated controls. Furthermore, immunological staining of FOXP3, CD4 and CD8 showed no marked difference in immune cell infiltrate in tumor environment following treatment. Taken together, a survival advantage of VQ-1 treatment alone could not be demonstrated here, even though some effect upon tumor size was seen. Staining for immune cell markers did not indicate that VQ-1 either reduced or increased host anti-tumor immune response.

  19. Ginsenoside Rg3 enhances radiosensitization of hypoxic oesophageal cancer cell lines through vascular endothelial growth factor and hypoxia inducible factor 1α.

    Science.gov (United States)

    Ge, Xiaolin; Zhen, Fuxi; Yang, Baixia; Yang, Xi; Cai, Jing; Zhang, Chi; Zhang, Sheng; Cao, Yuandong; Ma, Jianxin; Cheng, Hongyan; Sun, Xinchen

    2014-06-01

    To determine if the pretreatment of hypoxic human oesophageal carcinoma cell lines (EC109, TE1 and KYSE170) with ginsenoside Rg3 (Rg3) increases their radiosensitivity to X-rays. The growth inhibitory effect of different Rg3 concentrations was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Radiation sensitivity was measured using a clone formation assay and flow cytometry was used to measure the effects of Rg3 on radiation-induced apoptosis. Western blot analysis was used to measure the effects of Rg3 on the levels of hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF). Rg3 inhibited EC109, TE1 and KYSE170 cell growth in a dose- and time-dependent manner. Pretreatment with 10 µmol/ml Rg3 increased EC109, TE1 and KYSE170 radiosensitivity. Rg3 plus radiation significantly increased the apoptosis rate compared with radiation alone. Rg3 also decreased VEGF and HIF-1α protein levels in EC109 cells in a dose-dependent manner. The combination of Rg3 and radiation increased the fragmentation of double-stranded DNA. Rg3 enhanced the radiosensitivity of human oesophageal carcinoma cell lines cultured under hypoxic conditions possibly by downregulating VEGF and HIF-1α protein levels. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  20. Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells

    Directory of Open Access Journals (Sweden)

    Gang Li

    2014-01-01

    Full Text Available The aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells. 20(R-ginsenoside Rg3 (20(R-Rg3 and 20(S-ginsenoside Rg3 (20(S-Rg3 were prepared by the method of chemical degradation and column chromatography, and the structure of the two compounds was characterized by 1H-NMR and 13C-NMR spectroscopy. MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h. We also investigated the changes of intracellular MDA content, SOD activity, and ROS formation after the treatment of ginsenoside Rg3 for 20 h. The results indicated that both 20 (R-Rg3 and 20 (S-Rg3 had obvious protection against H2O2-induced oxidative stress in SK-N-SH cells. Moreover, 20(R-Rg3 exhibited better antioxidant activity than 20(S-Rg3 in vitro. These findings are expected to provide some implication for further research and application of ginsenoside Rg3 in neuroprotection.

  1. Bioética e Doação e Transplante de Órgãos

    OpenAIRE

    Goldim, José Roberto

    2016-01-01

    Slide presentation about Bioethics, Organ Donations and Transplants. A brief review about ethical issues retated to organ transplantation.Apresentação de diapositivos sobre Bioética, Doação e Transplante de Órgãos. É uma breve apresentação dobre os aspectos éticos relacionados ao transplante de órgãos. 

  2. La expresión neonatal de microRNAs como potenciales biomarcadores tempranos del síndrome metabólico

    Directory of Open Access Journals (Sweden)

    P. Rodil-García

    2015-01-01

    Full Text Available El síndrome metabólico es un trastorno caracterizado por obesidad, hipertensión arterial, hipercolesterolemia, hiperlipidemia y alteraciones en el metabolismo de la glucosa, y constituye un factor de riesgo para el desarrollo de diabetes y enfermedades cardiovasculares. Las condiciones nutricionales, hormonales y metabólicas de la madre durante el embarazo y el posparto temprano pueden condicionar el desarrollo de enfermedades metabólicas en la vida adulta de los hijos. Este concepto, llamado “programación fetal”, sugiere la existencia de marcadores moleculares que permitirían estimar de forma temprana el riesgo de desarrollar síndrome metabólico e implementar medidas para prevenirlo. Este trabajo revisa el potencial de los microRNAs como biomarcadores para la detección temprana del síndrome metabólico dada su especificidad y estabilidad, su presencia en fluidos biológicos de obtención rutinaria en la clínica y su expresión temprana en diversas enfermedades.

  3. Nutrición y síndrome metabólico.

    Directory of Open Access Journals (Sweden)

    Pilar Matía Martín

    2007-01-01

    Full Text Available Existe suficiente evidencia sobre la asociación de alteraciones en el metabolismo de la glucosa, las lipoproteínas, la acción de la insulina, la hipertensión y la obesidad de distribución central. Esta asociación se denomina Síndrome Metabólico. A pesar de que ha sido cuestionada su existencia por la ADA y la EASD, es una herramienta útil que permite identificar a las personas que tienen un alto riesgo de desarrollar enfermedad cardiovascular. El síndrome metabólico y sus componentes individuales se asocian a una elevada incidencia de enfermedad cardiovascular. La obesidad y el sedentarismo son factores de riesgo subyacentes en la ruta patogénica de este síndrome, por tanto la modificación de los hábitos de vida es una intervención de primera línea en la prevención y tratamiento de la resistencia insulínica, la hiperglucemia, la dislipemia aterogénica y la hipertensión arterial. La reducción ponderal y el ejercicio son las claves del plan global, pero entre los tratamientos no farmacológicos la dieta permanece como una de las estrategias de reducción del riesgo cardiovascular más importantes. Estudios epidemiológicos han observado que una ingesta elevada de azúcares simples, de alimentos con alto índice glucémico y de dietas con alta carga glucémica se asocian a resistencia insulínica, diabetes mellitus tipo 2, hipertrigliceridemia y cifras bajas de colesterol- HDL. Un bajo consumo de grasa saturada a favor de ácidos grasos poliinsaturados y monoinsaturados se ha implicado en una reducción de la incidencia de diabetes mellitus tipo 2 y dislipemia, aunque continúa el debate. La fibra dietética de cereales no refinados ha sido beneficiosa en la reducción del riesgo de diabetes. Entre los patrones dietéticos, la dieta mediterránea se ha visto relacionada con una menor incidencia de diabetes y con una reducción del riesgo de muerte. Estudios de intervención para la prevención de diabetes tipo 2 han propuesto

  4. Síndrome metabólico: una mirada interdisciplinaria Metabolic syndrome: an interdisciplinary outlook

    Directory of Open Access Journals (Sweden)

    María del P Barrera

    2008-06-01

    Full Text Available El síndrome metabólico se caracteriza por la presencia de alteraciones como resistencia a la insulina, que se manifiestan por hiperinsulinismo y asociación con obesidad, diabetes mellitus tipo 2, hipertensión arterial y dislipidemia. Existen, entre otros, factores de riesgo como sobrepeso, obesidad, ingestión alta de carbohidratos y grasa, tabaquismo y sedentarismo. Diferentes grupos revisaron el concepto de síndrome metábólico: Organización Mundial de la Salud, Grupo Europeo para el Estudio de la Resistencia a la Insulina, Asociación Americana de Endocrinólogos Clínicos, Panel de expertos en detección, evaluación y tratamiento del colesterol alto en adultos y Federación Internacional de Diabetes. Estudios epidemiológicos reportan diversas cifras en prevalencia dependiendo de la población estudiada y del criterio diagnóstico; sin embargo, todos ellos muestran una problemática importante de salud pública. El eje de las alteraciones del síndrome está en la resistencia a la insulina que involucra un estado proinflamatorio que disminuye la capacidad de la insulina para ejercer las acciones en los órganos blanco. El tejido adiposo visceral sintetiza gran cantidad de hormonas e interleuquinas que influyen en las alternaciones metabólicas asociadas. Las medidas de prevención primaria y secundaria son necesarias para reducir la morbimortalidad y con ello lograr efectos importantes en la sociedad y en la calidad de vida de los individuos. El tratamiento contempla aspectos no farmacológicos y la prescripción de medicamentos en caso de requerirse. El tratamiento farmacológico debe considerar el control de los factores de riesgo cardiovascular.The metabolic syndrome is characterized by the presence of alterations such as insulin resistance manifested by hyperinsulinism and its association with pathologies like obesity, diabetes mellitus type 2, arterial hypertension and dyslipidemia. There are other risk factors like overweight

  5. Síndrome metabólica e artrite idiopática juvenil Metabolic syndrome and juvenile idiopathic arthritis

    Directory of Open Access Journals (Sweden)

    Clarisse de Almeida Zanette

    2010-04-01

    Full Text Available A Artrite Idiopática Juvenil (AIJ é a artropatia crônica mais prevalente na infância e na adolescência. A prevalência da síndrome metabólica, assim como da obesidade, vem apresentando rápido aumento, atingindo todas as faixas etárias, inclusive a infância. A síndrome metabólica é caracterizada por um conjunto de riscos para doença cardiovascular e diabetes melito tipo 2, abrangendo adiposidade abdominal, resistência à insulina, dislipidemias e hipertensão arterial sistêmica. Além desses componentes, a inflamação tem sido reconhecida cada vez mais como um fator importante na síndrome metabólica e na obesidade, e pacientes com doenças caracterizadas por processos inflamatórios crônicos, como a AIJ, poderiam representar grupos de risco especiais. Os glicocorticoides são utilizados rotineiramente no controle da inflamação da AIJ, em doses elevadas e com uso prolongado. O uso crônico do glicocorticoide pode induzir resistência à insulina, hipertensão arterial sistêmica e obesidade, aumentando o risco de desenvolver síndrome metabólica. O objetivo deste artigo é revisar a literatura sobre a prevalência dos diversos componentes da síndrome metabólica em pacientes com AIJ. Observamos que, nesses pacientes, os dados sobre síndrome metabólica e seus componentes são muito escassos e mais estudos se fazem necessários, tendo em vista o potencial impacto no aumento do risco de doença cardiovascular.Juvenile idiopathic arthritis (JIA is the most prevalent chronic arthropathy in childhood and adolescence. The prevalence of metabolic syndrome, as well as obesity, is increasing rapidly in all age groups, including children. Metabolic syndrome is defined as a cluster of risk factors for cardiovascular disease and type 2 diabetes mellitus, including abdominal obesity, insulin resistance, dyslipidemia and hypertension. Besides those components, inflammation has been increasingly considered as a significant component of

  6. The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice

    Directory of Open Access Journals (Sweden)

    Yanqing Gu

    2014-01-01

    Full Text Available Ginsenoside Rg1 is a natural product extracted from Panax ginseng C.A. Although Rg1 protects tissue structure and functions by inhibiting local inflammatory reaction, the mechanism remains poorly understood. In vitro, Rg1 dose-dependently inhibited TRAP activity in receptor activator of nuclear factor-κB ligand- (RANKL- induced osteoclasts and decreased the number of osteoclasts and osteoclast resorption area. Rg1 also significantly inhibited the RANK signaling pathway, including suppressing the expression of Trap, cathepsin K, matrix metalloproteinase 9 (MMP9, and calcitonin receptor (CTR. In vivo, Rg1 dramatically decreased arthritis scores in CIA mice and effectively controlled symptoms of inflammatory arthritis. Pathologic analysis demonstrated that Rg1 significantly attenuated pathological changes in CIA mice. Pronounced reduction in synovial hyperplasia and inflammatory cell invasion were observed in CIA mice after Rg1 therapy. Alcian blue staining results illustrated that mice treated with Rg1 had significantly reduced destruction in the articular cartilage. TRAP and cathepsin K staining results demonstrated a significant reduction of numbers of OCs in the articular cartilage in proximal interphalangeal joints and ankle joints in Rg1-treated mice. In summary, this study revealed that Rg1 reduced the inflammatory destruction of periarticular bone by inhibiting differentiation and maturation of osteoclasts in CIA mice.

  7. Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways

    Directory of Open Access Journals (Sweden)

    Yang Li

    2017-01-01

    Full Text Available The ginsenoside Rg1 exerts a neuroprotective effect during cerebral ischemia/reperfusion injury. Rg1 has been previously reported to improve PPARγ expression and signaling, consequently enhancing its regulatory processes. Due to PPARγ’s role in the suppression of oxidative stress and inflammation, Rg1’s PPARγ-normalizing capacity may play a role in the observed neuroprotective action of Rg1 during ischemic brain injury. We utilized a middle cerebral artery ischemia/reperfusion injury model in rats in addition to an oxygen glucose deprivation model in cortical neurons to elucidate the mechanisms underlying the neuroprotective effects of Rg1. We found that Rg1 significantly increased PPARγ expression and reduced multiple indicators of oxidative stress and inflammation. Ultimately, Rg1 treatment improved neurological function and diminished brain edema, indicating that Rg1 may exert its neuroprotective action on cerebral ischemia/reperfusion injury through the activation of PPARγ signaling. In addition, the present findings suggested that Rg1 was a potent PPARγ agonist in that it upregulated PPARγ expression and was inhibited by GW9662, a selective PPARγ antagonist. These findings expand our previous understanding of the molecular basis of the therapeutic action of Rg1 in cerebral ischemic injury, laying the ground work for expanded study and clinical optimization of the compound.

  8. Acidosis metabólica: un reto para los intensivistas Metabolic acidosis: a challenge for intensive care specialists

    Directory of Open Access Journals (Sweden)

    Isabel V. Hidalgo Acosta

    2005-06-01

    Full Text Available La acidosis es una manifestación de trastornos metabólicos en el organismo, la cual puede reflejar hipovolemia, hipoxia, sepsis y utilización del metabolismo alternativo en la producción de energía. Su diagnóstico precoz y sobre todo la prevención en el paciente críticamente enfermo condicionan la evolución de este. Existen varias clasificaciones que tratan de reflejar el estado metabólico y hemodinámico del paciente en los servicios de terapia intensiva, sin embargo el problema se presenta en el momento de atender un paciente. Restaurar volumen, alimentar, oxigenar o usar bicarbonato, podrían ser opciones terapéuticas. El problema radica en cuándo hacerlo y cómo. Realizamos esta revisión con el objetivo de actualizar a nuestros médicos con respecto al conocimiento, diagnóstico y atención de la acidosis metabólica en la sepsisAcidosis is a manifestation of metabolic disorders in the organism that may reflect hypovolemia, hypoxia, sepsis and utilization of alternative metabolism in the production of energy. Its early diagnosis, and mainly the prevention in the critically ill patient condition its evolution. There are various classifications that try to show the metabolic and hemodynamic state of the patient at the intensive care services; however, the problem appears at the moment of giving attention to a patient. To restore volume, to nourish, to oxygenate, or to use bicarbonate could be therapeutic options. The problem is when and how to do it. This review is made aimed at updating our physicians as regards knowledge, diagnosis and attention to metabolic acidosis in sepsis

  9. Suplementos nutricionales como modificadores del riesgo cardiovascular en componentes del síndrome metabólico en adultos

    OpenAIRE

    Tassinari, Stefano; Azuero, Andrés; Arreaza, Dan; Rueda-Rodríguez, María C.; Castañeda-Cardona, Camilo; Rosselli, Diego

    2016-01-01

    Objetivo: analizar los suplementos nutricionales con ácidos grasos de cadena larga, micronutrientes y antioxidantes en la población adulta, como posibles modificadores del riesgo cardiovascular en pacientes con diagnóstico de síndrome metabólico, o alguno de sus componentes. Métodos: Se realizó una revisión de la literatura en las bases de datos Medline y Embase, utilizando los términos “Metabolic Syndrome” AND “Dietary supplements” y ‘Metabolic Syndrome’/exp AND ‘Dietary supplement’/exp, ...

  10. Balanços metabólicos do enxofre em pacientes com leucodistrofia metacromática

    Directory of Open Access Journals (Sweden)

    Horacio M. Canelas

    1968-12-01

    Full Text Available Foram estudados os balanços metabólicos do enxofre em dois pacientes com a forma juvenil da leucodistrofia metacromática. Foi verificado, em ambos os casos, um balanço positivo desse metaloide, aparentemente não influenciado pelo tipo de dieta (geral ou vegetal. Este resultado está de acordo com a atual concepção patogênica dessa moléstia, que consiste, essencialmente, em uma sulfatidose com diminuição da atividade das sulfatases.

  11. O sistema endocanabinóide: novo paradigma no tratamento da síndrome metabólica

    OpenAIRE

    Godoy-Matos, Amélio F. de; Guedes, Erika Paniago; Souza, Luciana Lopes de; Valério, Cynthia Melissa

    2006-01-01

    O balanço energético é um dos mais importantes mecanismos de homeostase e de sobrevivência das espécies. O sistema endocanabinóide é um novo e importante componente entre estes mecanismos. Os seus receptores e agonistas endógenos se expressam no sistema nervoso central (SNC) e perifericamente, em vários sítios, estabelecendo uma rede de comunicação periferiaSNC. Um aspecto marcante é a sua expressão no tecido adiposo, onde regula a lipogênese e aumenta a expressão de genes influentes no metab...

  12. Síndrome metabólico y su relación con hipogonadismo tardío

    OpenAIRE

    Dra. A. Verónica Araya Q.

    2014-01-01

    Existen evidencias clínicas y experimentales que muestran una asociación entre niveles bajos de testosterona (T) e insulino resistencia (IR), síndrome metabólico y diabetes tipo 2. La etiología no ha sido completamente dilucidada pero, diversos estudios muestran que el mecanismo sería bidireccional. La IR puede manifestarse en el eje hipotálamo-hipófisis-testículo (HHT), disminuyendo la respuesta testicular a LH y la secreción de testosterona (T). Por otra parte, la disminudón de T favorece l...

  13. Síndrome metabólico. Asociación entre GLP-1 y factores de riesgo cardiovascular

    OpenAIRE

    Pérez-Durillo, Francsco Tomás

    2017-01-01

    [ES] Objetivos: Determinar la variación de niveles plasmáticos de péptido similar al glucagón (GLP-1) y de actividad dipeptidil peptidasa 4 (DPP4) en pacientes con síndrome metabólico (SM). Diseño: Estudio descriptivo transversal. 46 sujetos (entre 50-65 años) distribuidos en grupo SM y grupo sin SM. Resultados: Los niveles postprandiales de GLP-1 fueron superiores en hombres con SM respecto a las mujeres y en mujeres sin SM respecto a los hombres. Los pacientes diagnosticados de SM de ...

  14. Avaliação metabólica de mulheres jovens com hiperplasia adrenal congênita

    OpenAIRE

    Gabrielle Sormanti Schnaider Rezek

    2011-01-01

    Resumo: Introdução: Pacientes adultos com hiperplasia adrenal congênita (HAC) por deficiência de 21-hidroxilase apresentam maior incidência de obesidade, adiposidade visceral, hiperinsulinismo, resistência insulínica e hiperandrogenismo que indivíduos normais. A disfunção adrenomedular e o hipercortisolismo intermitente parecem estar associados a estas anormalidades, o que predispõe estes pacientes a um risco aumentado de desenvolvimento de síndrome metabólica e doença aterosclerótica. Objeti...

  15. Síndrome metabólico: concepto y aplicación práctica

    Directory of Open Access Journals (Sweden)

    Juan Carlos Lizarzaburu Robles

    2013-10-01

    Full Text Available El síndrome metabólico (SM es un conjunto de anormalidades metabólicas consideradas como un factor de riesgo para desarrollar enfermedad cardiovascular y diabetes. Los componentes del SM se han definido según diferentes guías y consensos. Las definiciones propuestas por el National Cholesterol Education Program Adult Treatment Panel III (ATP III y la International Diabetes Federation (IDF son las más utilizadas en las diferentes publicaciones; sin embargo, se han realizado actualizaciones para diferentes poblaciones según la etnia y ubicación geográfica, como es el caso de la Asociación Latinoamericana de Diabetes (ALAD, que define un perímetro abdominal determinado para la región América Latina. En el año 2009, la publicación Harmonizing the Metabolic Syndrome sugirió un consenso para el diagnóstico de SM tratando de unificar los criterios de las diferentes organizaciones. Respecto a la fisiopatología del SM, la resistencia a insulina ha sido considerada como base del desarrollo del conjunto de anormalidades que lo conforman, sugiriendo a la obesidad abdominal o central como responsable del desarrollo de la insulino resistencia. Las adipoquinas producidas por el tejido adiposo abdominal actuarían directa o indirectamente en el desarrollo de los componentes del síndrome. Es importante mencionar que el síndrome metabólico ha sido considerado un equivalente diagnóstico de prediabetes, por ser predictor de diabetes. El incremento en la prevalencia del SM a nivel mundial le ha otorgado una gran importancia en la prevención y control de riesgo de la enfermedad cardiovascular y la diabetes. En el presente artículo revisaremos importantes aspectos sobre la definición y diagnóstico del síndrome metabólico.

  16. Produtos naturais ativadores de PPAR e marcadores associados ao processo inflamatório na Síndrome Metabólica

    Directory of Open Access Journals (Sweden)

    L.F. Brito

    2013-01-01

    Full Text Available O processo inflamatório é o elo entre a síndrome metabólica e as doenças cardiovasculares. Para verificar a presença e o grau da inflamação, vários biomarcadores têm sido propostos e investigados. Este trabalho tem como objetivo revisar as recentes pesquisas que associam alguns marcadores expressos no tecido adiposo, enfatizando, dentre eles, a adiponectina, a resistina, a leptina e o transportador de glicose GLUT-4 na síndrome metabólica, a relação da inflamação decorrente desse conjunto de desordens metabólicas sob os receptores proliferadores peroxissomais (PPARs, bem como o efeito de diferentes extratos vegetais e produtos naturais bioativos na ativação desses receptores.

  17. Fatores associados à síndrome metabólica em adultos atendidos em um ambulatório de nutrição = Factors associated with metabolic syndrome in adults attending a nutrition outpatient clinic

    Directory of Open Access Journals (Sweden)

    Hess, Simone

    2014-01-01

    Conclusões: O estudo revelou uma elevada prevalência de síndrome metabólica nos adultos que demandam atendimento nutricional. A presença da síndrome metabólica esteve associada à maior idade, menor escolaridade, tabagismo e maior índice de massa corporal

  18. Advantageous use of HepaRG cells for the screening and mechanistic study of drug-induced steatosis

    Energy Technology Data Exchange (ETDEWEB)

    Tolosa, Laia [Unidad de Hepatología Experimental, Instituto de Investigación Sanitaria La Fe, Valencia 46026 (Spain); Gómez-Lechón, M. José [Unidad de Hepatología Experimental, Instituto de Investigación Sanitaria La Fe, Valencia 46026 (Spain); CIBERehd, FIS, Barcelona 08036 (Spain); Jiménez, Nuria [Unidad de Hepatología Experimental, Instituto de Investigación Sanitaria La Fe, Valencia 46026 (Spain); Hervás, David [Biostatistics Unit, Instituto de Investigación Sanitaria La Fe, Valencia 46026 (Spain); Jover, Ramiro [Unidad de Hepatología Experimental, Instituto de Investigación Sanitaria La Fe, Valencia 46026 (Spain); CIBERehd, FIS, Barcelona 08036 (Spain); Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Valencia, Valencia 46010 (Spain); Donato, M. Teresa, E-mail: donato_mte@gva.es [Unidad de Hepatología Experimental, Instituto de Investigación Sanitaria La Fe, Valencia 46026 (Spain); CIBERehd, FIS, Barcelona 08036 (Spain); Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Valencia, Valencia 46010 (Spain)

    2016-07-01

    Only a few in vitro assays have been proposed to evaluate the steatotic potential of new drugs. The present study examines the utility of HepaRG cells as a cell-based assay system for screening drug-induced liver steatosis. A high-content screening assay was run to evaluate multiple toxicity-related cell parameters in HepaRG cells exposed to 28 compounds, including drugs reported to cause steatosis through different mechanisms and non-steatotic compounds. Lipid content was the most sensitive parameter for all the steatotic drugs, whereas no effects on lipid levels were produced by non-steatotic compounds. Apart from fat accumulation, increased ROS production and altered mitochondrial membrane potential were also found in the cells exposed to steatotic drugs, which indicates that all these cellular events contributed to drug-induced hepatotoxicity. These findings are of clinical relevance as most effects were observed at drug concentrations under 100-fold of the therapeutic peak plasmatic concentration. HepaRG cells showed increased lipid overaccumulation vs. HepG2 cells, which suggests greater sensitivity to drug-induced steatosis. An altered expression profile of transcription factors and the genes that code key proteins in lipid metabolism was also found in the cells exposed to drugs capable of inducing liver steatosis. Our results generally indicate the value of HepaRG cells for assessing the risk of liver damage associated with steatogenic compounds and for investigating the molecular mechanisms involved in drug-induced steatosis. - Highlights: • HepaRG cells were explored as an in vitro model to detect steatogenic potential. • Multiple toxicity-related endpoints were analysed by HCS. • HepaRG showed a greater sensitivity to drug-induced steatosis than HepG2 cells. • Changes in the expression of genes related to lipid metabolism were revealed. • HepaRG allow mechanistic understanding of liver damage induced by steatogenic drugs.

  19. Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1.

    Directory of Open Access Journals (Sweden)

    Chien-Wen Hou

    Full Text Available The purpose of the study was to determine the effect of ginseng-based steroid Rg1 on TNF-alpha and IL-10 gene expression in human skeletal muscle against exercise challenge, as well as on its ergogenic outcomes. Randomized double-blind placebo-controlled crossover trials were performed, separated by a 4-week washout. Healthy young men were randomized into two groups and received capsule containing either 5 mg of Rg1 or Placebo one night and one hour before exercise. Muscle biopsies were conducted at baseline, immediately and 3 h after a standardized 60-min cycle ergometer exercise. While treatment differences in glycogen depletion rate of biopsied quadriceps muscle during exercise did not reach statistical significance, Rg1 supplementations enhanced post-exercise glycogen replenishment and increased citrate synthase activity in the skeletal muscle 3 h after exercise, concurrent with improved meal tolerance during recovery (P<0.05. Rg1 suppressed the exercise-induced increases in thiobarbituric acids reactive substance (TBARS and reversed the increased TNF-alpha and decreased IL-10 mRNA of quadriceps muscle against the exercise challenge. PGC-1 alpha and GLUT4 mRNAs of exercised muscle were not affected by Rg1. Maximal aerobic capacity (VO2max was not changed by Rg1. However, cycling time to exhaustion at 80% VO2max increased significantly by ~20% (P<0.05.Our result suggests that Rg1 is an ergogenic component of ginseng, which can minimize unwanted lipid peroxidation of exercised human skeletal muscle, and attenuate pro-inflammatory shift under exercise challenge.

  20. Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1.

    Science.gov (United States)

    Hou, Chien-Wen; Lee, Shin-Da; Kao, Chung-Lan; Cheng, I-Shiung; Lin, Yu-Nan; Chuang, Sheng-Ju; Chen, Chung-Yu; Ivy, John L; Huang, Chih-Yang; Kuo, Chia-Hua

    2015-01-01

    The purpose of the study was to determine the effect of ginseng-based steroid Rg1 on TNF-alpha and IL-10 gene expression in human skeletal muscle against exercise challenge, as well as on its ergogenic outcomes. Randomized double-blind placebo-controlled crossover trials were performed, separated by a 4-week washout. Healthy young men were randomized into two groups and received capsule containing either 5 mg of Rg1 or Placebo one night and one hour before exercise. Muscle biopsies were conducted at baseline, immediately and 3 h after a standardized 60-min cycle ergometer exercise. While treatment differences in glycogen depletion rate of biopsied quadriceps muscle during exercise did not reach statistical significance, Rg1 supplementations enhanced post-exercise glycogen replenishment and increased citrate synthase activity in the skeletal muscle 3 h after exercise, concurrent with improved meal tolerance during recovery (P<0.05). Rg1 suppressed the exercise-induced increases in thiobarbituric acids reactive substance (TBARS) and reversed the increased TNF-alpha and decreased IL-10 mRNA of quadriceps muscle against the exercise challenge. PGC-1 alpha and GLUT4 mRNAs of exercised muscle were not affected by Rg1. Maximal aerobic capacity (VO2max) was not changed by Rg1. However, cycling time to exhaustion at 80% VO2max increased significantly by ~20% (P<0.05). Our result suggests that Rg1 is an ergogenic component of ginseng, which can minimize unwanted lipid peroxidation of exercised human skeletal muscle, and attenuate pro-inflammatory shift under exercise challenge.

  1. Depth Discrimination Using Rg-to-Sg Spectral Amplitude Ratios for Seismic Events in Utah Recorded at Local Distances

    Energy Technology Data Exchange (ETDEWEB)

    Tibi, Rigobert [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Koper, Keith D. [Univ. of Utah, Salt Lake City, UT (United States). Dept. of Geology and Geophysics; Pankow, Kristine L. [Univ. of Utah, Salt Lake City, UT (United States). Dept. of Geology and Geophysics; Young, Christopher J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2018-03-20

    Short-period fundamental-mode Rayleigh waves (Rg) are commonly observed on seismograms of anthropogenic seismic events and shallow, naturally occurring tectonic earthquakes (TEs) recorded at local distances. In the Utah region, strong Rg waves traveling with an average group velocity of about 1.8 km/s are observed at ~1 Hz on waveforms from shallow events ( depth<10 km ) recorded at distances up to about 150 km. At these distances, Sg waves, which are direct shear waves traveling in the upper crust, are generally the dominant signals for TEs. Here in this study, we leverage the well-known notion that Rg amplitude decreases dramatically with increasing event depth to propose a new depth discriminant based on Rg-to-Sg spectral amplitude ratios. The approach is successfully used to discriminate shallow events (both earthquakes and anthropogenic events) from deeper TEs in the Utah region recorded at local distances ( <150 km ) by the University of Utah Seismographic Stations (UUSS) regional seismic network. Using Mood’s median test, we obtained probabilities of nearly zero that the median Rg-to-Sg spectral amplitude ratios are the same between shallow events on the one hand (including both shallow TEs and anthropogenic events), and deeper earthquakes on the other, suggesting that there is a statistically significant difference in the estimated Rg-to-Sg ratios between the two populations. We also observed consistent disparities between the different types of shallow events (e.g., mining blasts vs. mining-induced earthquakes), implying that it may be possible to separate the subpopulations that make up this group. Lastly, this suggests that using local distance Rg-to-Sg spectral amplitude ratios one can not only discriminate shallow events from deeper events but may also be able to discriminate among different populations of shallow events.

  2. Advantageous use of HepaRG cells for the screening and mechanistic study of drug-induced steatosis

    International Nuclear Information System (INIS)

    Tolosa, Laia; Gómez-Lechón, M. José; Jiménez, Nuria; Hervás, David; Jover, Ramiro; Donato, M. Teresa

    2016-01-01

    Only a few in vitro assays have been proposed to evaluate the steatotic potential of new drugs. The present study examines the utility of HepaRG cells as a cell-based assay system for screening drug-induced liver steatosis. A high-content screening assay was run to evaluate multiple toxicity-related cell parameters in HepaRG cells exposed to 28 compounds, including drugs reported to cause steatosis through different mechanisms and non-steatotic compounds. Lipid content was the most sensitive parameter for all the steatotic drugs, whereas no effects on lipid levels were produced by non-steatotic compounds. Apart from fat accumulation, increased ROS production and altered mitochondrial membrane potential were also found in the cells exposed to steatotic drugs, which indicates that all these cellular events contributed to drug-induced hepatotoxicity. These findings are of clinical relevance as most effects were observed at drug concentrations under 100-fold of the therapeutic peak plasmatic concentration. HepaRG cells showed increased lipid overaccumulation vs. HepG2 cells, which suggests greater sensitivity to drug-induced steatosis. An altered expression profile of transcription factors and the genes that code key proteins in lipid metabolism was also found in the cells exposed to drugs capable of inducing liver steatosis. Our results generally indicate the value of HepaRG cells for assessing the risk of liver damage associated with steatogenic compounds and for investigating the molecular mechanisms involved in drug-induced steatosis. - Highlights: • HepaRG cells were explored as an in vitro model to detect steatogenic potential. • Multiple toxicity-related endpoints were analysed by HCS. • HepaRG showed a greater sensitivity to drug-induced steatosis than HepG2 cells. • Changes in the expression of genes related to lipid metabolism were revealed. • HepaRG allow mechanistic understanding of liver damage induced by steatogenic drugs.

  3. Three-dimensional HepaRG model as an attractive tool for toxicity testing.

    Science.gov (United States)

    Leite, Sofia B; Wilk-Zasadna, Iwona; Zaldivar, Jose M; Airola, Elodie; Reis-Fernandes, Marcos A; Mennecozzi, Milena; Guguen-Guillouzo, Christiane; Chesne, Christopher; Guillou, Claude; Alves, Paula M; Coecke, Sandra

    2012-11-01

    The culture of HepaRG cells as three dimensional (3D) structures in the spinner-bioreactor may represent added value as a hepatic system for toxicological purposes. The use of a cost-effective commercially available bioreactor, which is compatible with high-throughput cell analysis, constitutes an attractive approach for routine use in the drug testing industry. In order to assess specific aspects of the biotransformation capacity of the bioreactor-based HepaRG system, the induction of CYP450 enzymes (i.e., CYP1A2, 2B6, 2C9, and 3A4) and the activity of the phase II enzyme, uridine diphosphate glucuronoltransferase (UGT), were tested. The long-term functionality of the system was demonstrated by 7-week stable profiles of albumin secretion, CYP3A4 induction, and UGT activities. Immunofluorescence-based staining showed formation of tissue-like arrangements including bile canaliculi-like structures and polar distribution of transporters. The use of in silico models to analyze the in vitro data related to hepatotoxic activity of acetaminophen (APAP) demonstrated the advantage of the integration of kinetic and dynamic aspects for a better understanding of the in vitro cell behavior. The bioactivation of APAP and its related cytotoxicity was assessed in a system compatible to high-throughput screening. The approach also proved to be a good strategy to reduce the time necessary to obtain fully differentiated cell cultures. In conclusion, HepaRG cells cultured in 3D spinner-bioreactors are an attractive tool for toxicological studies, showing a liver-like performance and demonstrating a practical applicability for toxicodynamic approaches.

  4. Synthesis of branched–backbone oligosaccharides of the pectic RG-I plant cell wall polysaccharide

    DEFF Research Database (Denmark)

    Awan, Shahid Iqbal; Clausen, Mads Hartvig

    with numerous branches of galactan, arabinan, or arabinogalactan positioned at C-4 of the rhamnose residues. The use of defined oligosaccharides rather than isolated polysaccharides can aid in obtaining detailedinformation about biosynthetic pathways, plant evolution, and agronomical properties. Furthermore......,biological testing can provide new insight into plant biology; important for plant preservation, engineering,and utilization of plants as a source of bioenergy. Present work towards defined RG-I substructures involvesa [4+3]-coupling to furnish a heptasaccharide backbone unit (see Figure 1). Moreover, installation...

  5. Shaping biomedical objects across history and philosophy:a conversation with Hans-Jörg Rheinberger.

    Science.gov (United States)

    García-Sancho, Miguel; González-Silva, Matiana; Jesús Santesmases, María; Rheinberger, Hans-Jörg

    2014-01-01

    Historical epistemology, according to the historian of science Hans-Jörg Rheinberger, is a space through which "to take experimental laboratory work into the realm of philosophy". This key concept, together with the crucial events and challenges of his career, were discussed in a public conversation which took place on the occasion of Rheinberger's retirement. By making sense of natural phenomena in the laboratory, the act of experimenting shapes the object; it is this shaping which became the core of Rheinberger's own research across biology and philosophy into history. For his intellectual agenda, a history of the life sciences so constructed became "epistemologically demanding".

  6. Biomaterials in medical devices: an interview with Jörg Vienken of Fresenius Medical Care, Germany.

    Science.gov (United States)

    Vienken, Jörg

    2012-06-01

    Biomaterial and biopolymer research have significant impact on the development as well as application of biotechnology. Biotechnology Journal recently attended the "Nanomaterials for Biomedical Technologies 2012" conference. We were privileged to have the opportunity to ask Prof. Dr. Jörg Vienken, VP of BioSciences at Fresenius Medical Care, a few questions relating to medical devices, the importance of publishing for industry, and also his advice for young scientists/engineers looking for a career in industry. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Importancia del grado de control metabólico en los resultados de la cirugía oftalmológica en pacientes diabéticos

    OpenAIRE

    González Gómez, María

    2012-01-01

    La diabetes mellitus (DM) por su implicación endocrino- metabólica consituye un factor de riesgo en el paciente quirúrgico. Afecta a diferentes órganos, siendo los ojos uno de los órganos más frecuentemente dañados, generando complicaciones en estructuras oculares incluso en la visión y dando lugar a diferentes patologías que requieren cirugía. el manejo terapeútico y de control metabólico del paciente diabético antes, durante y después del acto quirúrgico posee una trascendencia esencial p...

  8. Relación entre síndrome metabólico e índice neutrófilo/linfocito

    OpenAIRE

    Copca-Nieto, DV; Álvarez-López, JA; Santillán-Fragoso, WJ; Ramírez-del Pilar, R; López y López, LR; López-González, DS; Lagunas-Alvarado, M; López-Alférez, R; Martínez-Ramos-Méndez, M; Reyes-Jiménez, AE; Alba-Rangel, DL; Terán-González, JO; Castro-D'Franchis, LJ

    2017-01-01

    Resumen ANTECEDENTES: el síndrome metabòlico agrupa factores de riesgo de diabetes mellitus 2 y enfermedad cardiovascular: obesidad central, hiperglucemia, hipertensión arterial, hipertrigliceridemia y colesterol de alta densidad (C-HDL) reducido. La obesidad y el síndrome metabòlico son afecciones en las que sobreviene inflamación subclínica crónica, factor de riesgo independiente de enfermedad cardiovascular y aterosclerosis. El índice neutrófilo/linfocito (INL) ha surgido como un marcador...

  9. Síndrome metabólico y calidad de vida en pacientes con psoriasis. Hospital Arzobispo Loayza Enero - Julio 2015.

    OpenAIRE

    Castro Rodríguez, María del Carmen

    2017-01-01

    Determina la asociación entre la severidad de psoriasis con el síndrome metabólico y calidad de vida en los pacientes con dicha enfermedad que acudieron al Servicio de Dermatología del Hospital Nacional Arzobispo Loayza, periodo enero a julio 2015. Se realizó un estudio analítico, observacional y transversal en 97 pacientes mayores de edad con diagnóstico de psoriasis, se evaluó la asociación del síndrome metabólico, calidad de vida y severidad de la psoriasis (criterios de la American Health...

  10. Efecto del Síndrome Metabólico inducido por una dieta rica en fructosa sobre el tejido óseo

    OpenAIRE

    Felice, Juan Ignacio

    2014-01-01

    Introducción. El Síndrome Metabólico (SM) se define como un conjunto de anormalidades fisiológicas y metabólicas que incluyen obesidad central y global, tolerancia a la glucosa alterada con insulinorresistencia, dislipidemia e hipertensión. Desde su descripción en 1988, la incidencia del SM ha aumentado dramáticamente, y se ha reportado que se presenta en más del 20% de la población adulta en los países en desarrollo. La prevalencia del SM aumenta con la edad y se asocia con alto riesgo de Di...

  11. Efecto de una bebida antioxidante sobre marcadores de estrés oxidativo y riesgo cardiovascular en pacientes con Síndrome Metabólico

    OpenAIRE

    Bernabé García, Juana

    2014-01-01

    El síndrome metabólico, conocido también como síndrome plurimetabólico, síndrome de resistencia a la insulina o síndrome X, es una entidad clínica controvertida que aparece con amplias variaciones fenotípicas en personas con una predisposición endógena, determinada genéticamente y condicionada por factores ambientales. El síndrome metabólico se caracteriza por la presencia de insulinoresistencia con hiperinsulinismo compensador asociado a trastornos del metabolismo hidrocarbonado, cifras elev...

  12. Alterações Ósseas e do Sistema Estomatognático associadas a Doenças Endócrino Metabólicas

    OpenAIRE

    Matos Paraguassú, Gardênia; Andrade Pimentel, Poliana; Sacramento dos Santos, Mércia; Morais Godoy Figueiredo, Leonardo; Pedreira Ramalho, Luciana Maria; Almeida Sarmento, Viviane

    2013-01-01

    Introdução: a prevalência de doenças endócrino-metabólicas tem aumentado nos últimos anos com a melhoria da expectativa de vida da população. Diabete Melittus, hipotireoidismo, hipertireoidismo, hipoparatireoidismo e hiperparatireoidismo podem levar as alterações no sistema estomatognático, contudo, a literatura ainda é limitada nesta área. Objetivo: Abordar as características e o processo fisiopatológico de doenças endócrino-metabólicas, com destaque para as alterações ósseas e do sistema es...

  13. Factores de riesgo ambientales y componentes del síndrome metabólico en adolescentes con exceso de peso

    Directory of Open Access Journals (Sweden)

    Nora Elena Múnera

    2012-03-01

    Conclusiones. Los factores de riesgo ambientales asociados con los componentes del síndrome metabólico en este estudio fueron el mayor consumo de calorías, carbohidratos complejos y simples, todos relacionados directamente con el IMC; por el contrario, el nivel de actividad física, los antecedentes familiares y los personales no mostraron ninguna asociación. El síndrome metabólico sólo se presentó en jóvenes con obesidad.   DOI: http://dx.doi.org/10.7705/biomedica.v32i1.595

  14. Correlación fenotipo-genotipo de variantes génicas en síndrome metabólico

    OpenAIRE

    Antolí Royo, Ana Cristina

    2016-01-01

    [ES]La importancia del Síndrome Metabólico viene dada por el aumento del riesgo cardiovascular que genera, con un aumento de la mortalidad, y por su alta prevalencia. Esto ha llevado a investigar diferentes factores genéticos que puedan estar implicados en la resistencia insulínica y la obesidad. Nuestro objetivo ha sido encontrar una asociación entre determinados polimorfismos genéticos, como son la IL-1β, IL-4, IL-6, PPAR-γ y TNF-α, y su relación con el Síndrome Metabólico (según l...

  15. Caracterización fenotípica y metabólica del síndrome metabólico en Cartagena de Indias Phenotypic and metabolic characterization of the metabolic syndrome in Cartagena de Indias

    Directory of Open Access Journals (Sweden)

    Fernando Manzur

    2008-06-01

    Full Text Available Introducción: el síndrome metabólico agrupa una serie de factores de riesgo que afectan a un mismo individuo y predisponen a enfermedad cardiovascular y diabetes mellitus tipo 2. Su detección y tratamiento precoces permiten mejorar los indicadores de salud de la población. Objetivo: describir la prevalencia del síndrome metabólico y sus componentes, a través de la comparación de los criterios del Tercer Panel de Tratamiento del Adulto (ATP III y los de la Federación Internacional de Diabetes (IDF, en adultos mayores de 30 años, oriundos de Cartagena de Indias. Métodos: estudio descriptivo de corte transversal, en el cual se evaluaron 749 personas de las diferentes zonas de Cartagena, por muestreo aleatorio, encuesta estructurada y consentimiento informado. Se les midió la presión arterial, y la circunferencia de la cintura, y en ayunas se determinaron niveles de glucemia, colesterol total, colesterol HDL y triglicéridos. Resultados: la muestra quedó conformada por 545 (73% mujeres y 204 (27% hombres, con edad promedio de 51,7 ± 13 años. Con los criterios del ATP III la prevalencia del síndrome metabólico fue de 25,4% y según los de la IDF 31,5%. Para los criterios comunes fueron: hipertrigliceridemia 21,1%; hipertensión arterial 49,1% y c-HDL bajo 55,1%. Las prevalencias para los componentes no comunes de obesidad central e hiperglucemia en ayunas ATP III versus IDF fueron: 41% vs. 71% y 10% vs. 13%, respectivamente. Cconclusiones: la presencia de síndrome metabólico fue mayor cuando se aplicaron los criterios de la IDF, en especial para el género masculino. Estos resultados muestran que el síndrome metabólico presenta una alta prevalencia en Cartagena de Indias, de allí la importancia de la promoción y prevención para mejorar la calidad de vida de esta población.Introduction: the metabolic syndrome gathers a series of risk factors that affect a subject and predispose to cardiovascular disease and diabetes mellitus

  16. Influence of postbiotic RG14 and inulin combination on cecal microbiota, organic acid concentration, and cytokine expression in broiler chickens.

    Science.gov (United States)

    Kareem, K Y; Loh, T C; Foo, H L; Asmara, S A; Akit, H

    2017-04-01

    This study examined the effects of different combinations of inulin and postbiotics RG14 on growth performance, cecal microbiota, volatile fatty acids (VFA), and ileal cytokine expression in broiler chickens. Two-hundred-and sixteen, one-day-old chicks were allocated into 6 treatment groups, namely, a basal diet (negative control, NC), basal diet + neomycin and oxytetracycline (positive control, PC), T1 = basal diet + 0.15% postbiotic RG14 + 1.0% inulin, T2 = basal diet + 0.3% postbiotic RG14 + 1.0% inulin, T3 = basal diet + 0.45% postbiotic RG14 + 1.0% inulin, and T4 = basal diet + 0.6% postbiotic RG14 + 1.0% inulin, and fed for 6 weeks. The results showed that birds fed T1 and T3 diets had higher (P  0.05) among diets. The NC birds had higher (P inulin combinations are potential replacements for antibiotic growth promoters in the poultry industry. © 2016 Poultry Science Association Inc.

  17. Influence of B-Complex Vitamins on the Pharmacokinetics of Ginsenosides Rg1, Rb1, and Ro After Oral Administration.

    Science.gov (United States)

    Zheng, Peihe; Chen, Yinbin; Fu, Yangyang; Wang, Hecheng; Wang, Jia; Zheng, Siwen; Xiao, Shengyuan; Wang, Yingping

    2017-11-01

    After cultivation of ginseng, ginsenosides, which are the major active ingredients of gingeng, were approved for use by the food industry, and began to be used as added functional ingredients to try to improve the quality and price of functional foods. However, the interaction between different types of ginsenosides and nutrients needs further study. We investigated the effect of B-complex vitamins (which are essential nutrients) on the pharmacokinetics of the ginsenosides protopanaxatriol-type saponin Rg 1 , protopanaxadiol-type saponin Rb 1 , and oleanolic acid-type saponin Ro after oral administration. Ginsenosides Rg 1 , Rb 1 , and Ro, with or without B-complex vitamins, respectively, were administered orally to rats to evaluate their pharmacokinetics. The concentration of ginsenosides in plasma was determined by liquid chromatography/tandem mass spectrometry. Pharmacokinetic parameters were fitted using WinNonlin v6.2. After oral coadministration with B-complex vitamins, the area under the concentration-time curve from zero to infinity (AUC 0-∞ ) of ginsenoside Rg 1 was reduced by 70%, that of ginsenoside Rb 1 was reduced by 43%, and that of ginsenoside Ro was reduced by 34%. The AUC 0-∞ of ginsenosides Rg 1 and Rb 1 showed significant differences between different treatments, but the AUC 0-∞ of ginsenoside Ro did not. These results suggest significant ginsenoside-nutrient interactions between ginsenosides Rg 1 , Rb 1 , and B-complex vitamins.

  18. Evidence for the strongest version of the 4d a-theorem via a-maximization along RG flows

    International Nuclear Information System (INIS)

    Barnes, Edwin; Intriligator, Ken; Wecht, Brian; Wright, Jason

    2004-01-01

    In earlier work, we (KI and BW) gave a two line 'almost proof' (for supersymmetric RG flows) of the weakest form of the conjectured 4d a-theorem, that aIRaUV, using our result that the exact superconformal R-symmetry of 4d SCFTs maximizes a=3TrR3-TrR. The proof was incomplete because of two identified loopholes: theories with accidental symmetries, and the fact that it is only a local maximum of a. Here we discuss and extend a proposal of Kutasov (which helps close the latter loophole) in which a-maximization is generalized away from the endpoints of the RG flow, with Lagrange multipliers that are conjectured to be identified with the running coupling constants. a-maximization then yields a monotonically decreasing 'a-function' along the RG flow to the IR. As we discuss, this proposal in fact suggests the strongest version of the a-theorem: that 4d RG flows are gradient flows of an a-function, with positive-definite metric. In the perturbative limit, the RG flow metric thus obtained is shown to agree precisely with that found by very different computations by Osborn and collaborators. As examples, we discuss a new class of 4d SCFTs, along with their dual descriptions and IR phases, obtained from SQCD by coupling some of the flavors to added singlets

  19. Örgütsel Bağlılık ile Örgütsel Sinizm ve Örgütsel Muhalefet Arasındaki İlişki The Relationship Between Organizational Commitment and Organizational Cynicism and Organizational Dissent

    Directory of Open Access Journals (Sweden)

    Kaya YILDIZ

    2013-07-01

    Full Text Available Organizatonal commitment is the teellings and relation employessto the organization they work. It is seen that organizational commitmentis affected by various factors and various factors are influenced byorganizational commitment. In recent years, organizational cynicismand organizational dissent are observed as new topic areasorganizational behaviour. In this regard, theachers’ cynicism anddissent perceptions are considered it is seen that to be important. Inthis framework, the aim of this study is to investigate the relationshipbetween primary school teachers’ organizational commitment andorganizational cynicism and organizational dissent perceptions. Thepopulation of the study is formed by primary school teachers (n:219working in the city center of Bolu in 2012-2013 academic year. Thedata were collected through “Organizational Commitment Scale”developed by Meyer and Allen (1984 adapted into Turkish by Boylu,Pelit and Güçer (2007 and “Organizational Cynicism Scale” developedby Brandes, Dharwadkar ve Dean (1999 adapted into Turkish byKalağan (2009 and Dissent Behaviours Sub-Scale of “OrganizationalDissent Scale” developed by Özdemir (2010. To analyze the data, SPSS16.0 program was used and descriptive statistics as; frequency,percentage, meanscore, standart deviation and correlation, were used.The findings of the study revealed correlation between primary schoolteachers’ organizational commitment and organizational cynicism andorganizational dissent perceptions. A high negative correlation wasfound between the organizational commitment and organizationalcynicism perceptions and a moderate negative correlation was foundbetween organizational commitment and organizational dissentperceptions of primary school teachers. Örgütsel bağlılık, işgörenin çalıştığı örgüte karşı hissettiği bağın bir ifadesidir. Örgütsel bağlılığın birçok değişkenden etkilendiği ve birçok değişkeni de etkilediği g

  20. Síndrome metabólico y puesto de trabajo Metabolic syndrome and workplace

    Directory of Open Access Journals (Sweden)

    Juan Carlos Palomino Baldeon

    2010-12-01

    Full Text Available Introducción: El Síndrome Metabólico (SM aumenta el riesgo de enfermedad cardiovascular y diabetes. Nuestro objetivo es conocer la prevalencia del SM en nuestra población y conocer la diferencia de prevalencias de SM entre trabajadores que trabajan en zona industrial y zona administrativa u oficina, independientemente de la ocupación específica. Materiales y Métodos: La población fue dividida en zona industrial (Tipo 1 y no industrial u oficina (Tipo 2. Es un estudio Caso Control; la exposición es el trabajo en zona industrial y la enfermedad es la presencia de SM. Se utilizó un modelo multivariado usando la regresión linear. Resultados: 891 trabajadores fueron evaluados, población Tipo 1 fue 722 (81,03% y población Tipo 2 fue 169 (18,97%. La prevalencia de SM fue 27,83%. La prevalencia de SM en población Tipo 1 y Tipo 2 fue de 28,94% y 23,07% respectivamente, p Introduction: The Metabolic Syndrome (MS raises Diabetes and Cardiovascular disease risks. Our goal is to know MS prevalence in our population and to know the difference of MS prevalence between workers that work in an industrial zone versus administrative or office zone independently of their specific occupation. Materials and methods: Population was divided in industrial zone (Type 1 and non industrial or office zone (Type 2. It is a Case-Control study; the exposure is the job in industrial zone and the disease is the presence of MS. A multivariate model using lineal regression was used. Results: 891 workers were evaluated, Type 1 population was 722 (81.03% and Type 2 population was 169 (18.97%. MS prevalence was 27.83%. Type 1 MS prevalence was 28.94% and Type 2 MS prevalence was 23.07%, p < 0.05. It was found p<0.01 for age, Body mass index (BMI, Arterial Tension, Glycemia, triglycerides and HDL measures. Relative risk of Type 1 job for complaint MS was 1.358 CI (1.19-2.45. Conclusion: Those Type 1 workers have more risk of MS because of high calories food consumption

  1. Artrite reumatoide e síndrome metabólica Rheumatoid arthritis and metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Viviane Roseli da Cunha

    2011-06-01

    Full Text Available Nos últimos 20 anos, tem sido demonstrado que a artrite reumatoide (AR pode reduzir a expectativa de vida de 3 a 10 anos em comparação com a população em geral. Atualmente, a doença cardiovascular (DCV é a principal causa de morte nos pacientes com AR, e a ocorrência de infarto agudo do miocárdio pode ser até quatro vezes maior nesses pacientes. A resposta inflamatória sistêmica de etiologia autoimune, somada à presença de síndrome metabólica (SM, torna duas vezes maior o risco de DCV fatal ou não fatal e da aterosclerose coronariana, independentemente da idade e sexo. A artrite reumatoide tem sido associada a aumento da prevalência de SM, mas o papel das diferentes características da doença, tais como a duração da doença, atividade e tratamento com glucocorticoides não estão bem definidos. O objetivo deste trabalho é revisar a prevalência de SM e os fatores implicados no desenvolvimento de aterosclerose nos pacientes com AR, avaliando os aspectos clínicos da AR e sua associação com o desenvolvimento de SM.In the past 20 years, the life expectancy of patients with rheumatoid arthritis (RA has been shown to be reduced by three to ten years as compared to that of the general population. Currently, cardiovascular disease (CVD is the major cause of death in patients with RA, and acute myocardial infarction can be up to four times more frequent in these patients. The autoimmune systemic inflammatory response, along with the presence of metabolic syndrome (MetS, doubles the risk for fatal or non-fatal CVD and coronary atherosclerosis, regardless of age and sex. Rheumatoid arthritis has been associated with increased prevalence of MetS, but its role in the different characteristics of the disease, such as disease duration, activity, and treatment with glucocorticoids, is not well defined. This study aimed at reviewing the prevalence of MetS and the factors implicated in the development of atherosclerosis in RA patients

  2. Topiramate and severe metabolic acidosis: case report Acidose metabólica grave por topiramato: relato de caso

    Directory of Open Access Journals (Sweden)

    Jayme E. Burmeister

    2005-06-01

    Full Text Available Topiramate infrequently induces anion gap metabolic acidosis through carbonic anhydrase inhibition on the distal tubule of the nephron - a type 2 renal tubular acidosis. We report on a 40 years old woman previously healthy that developed significant asymptomatic metabolic acidosis during topiramate therapy at a dosage of 100mg/day for three months. Stopping medication was followed by normalization of the acid-base status within five weeks. This infrequent side effect appears unpredictable and should be given careful attention.Topiramato pode produzir raramente uma acidose metabólica através da inibição da anidrase carbônica no túbulo distal do néfron - acidose tubular renal do tipo 2. Relatamos o caso de mulher de 40 anos previamente saudável que desenvolveu quadro de acidose metabólica assintomática grave, sem outra etiologia identificável, durante uso de topiramato na dose de 100mg/dia por três meses. Este efeito colateral, embora infrequente, parece ser imprevisível e requer atenção cuidadosa.

  3. Síndrome metabólico en la infancia, un enfoque para la atención primaria

    Directory of Open Access Journals (Sweden)

    Orlando Araujo Herrera

    Full Text Available La obesidad en niños y adolescentes es un problema de salud a nivel mundial, asociado frecuentemente a diferentes alteraciones clínicas, como el síndrome metabólico, la aterosclerosis temprana, la dislipidemia, la hipertensión arterial y la diabetes mellitus tipo 2. El síndrome metabólico ha sido definido como la asociación de varios factores de riesgo precursores de enfermedad cardiovascular arterosclerótica y de diabetes mellitus tipo 2 en el adulto, sin embargo no hay consenso respecto al diagnóstico en la infancia y la adolescencia. La importancia clínica de este síndrome y su detección temprana, se debe a su creciente prevalencia y a su elevado riesgo cardiovascular, que a su vez, se debe a la suma de sus componentes, que de manera independiente, constituyen factores de riesgo. El tratamiento en este grupo de edades tiene como objetivo la prevención primaria y promover cambios en el estilo de vida.

  4. Obesidad y síndrome metabólico en niños y adolescentes

    Directory of Open Access Journals (Sweden)

    Dr. Castillo-Durán Garlos

    2012-03-01

    Full Text Available La obesidad general y en niños es un problema de salud pública con claro aumento a nivel mundial y en Chile. En las edades pediátricas, aparte de sus consecuencias traumatológicas, metabólicas y psicológicas, ya tiene un impacto en el riesgo de enfermedades en las edades adultas. El concepto de síndrome metabólico está en uso hace algunos años en la edad adulta, para aunar factores de riesgo de llegar a desarrollar diabetes tipo II y enfermedad cardiovascular. En pediatría hay suficiente evidencia que son factores predictores de riesgo desde la edad escolar la mayoría de los componentes estudiados en adultos: aumento de circunferencia abdominal, de presión arterial sistólica y diastólica, de triglicéridos y disminución de colesterol HDL sérico. Están en estudio otros componentes del síndrome: resistencia insulínica, adiposidad intraabdominal, hiperuricemia, hiperleptinemia, grosor aumentado de íntimamedia arterial, signos sugerentes de hígado graso. Se justifica el estudio de estos factores en niños y adolescentes obesos y su manejo intensivo por un equipo profesional entrenado.

  5. 5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells in vitro

    Directory of Open Access Journals (Sweden)

    Krzysztof Bilmin

    2016-10-01

    Full Text Available Sonodynamic therapy (SDT is a promising technique based on the ability of certain substances, called sonosensitizers, to sensitize cancer cells to non-thermal effects of low-energy ultrasound waves, allowing their destruction. Sonosensitization is thought to induce cell death by direct physical effects such as cavitation and acoustical streaming as well as by complementary chemical reactions generating oxygen free radicals. One of the promising sonosensitizers is 5-aminolevulinic acid (ALA which upon selective uptake by cancer cells is metabolized and accumulated as protoporphyrin IX. The objective of the study was to describe ALA-mediated sonodynamic effects in vitro on a rat RG2 glioma cell line. Glioma cells, seeded at the bottom of 96-well plates and incubated with ALA (10 µg/ml for 6 h, were exposed to the sinusoidal US pulses with a resonance frequency of 1 MHz, 1000 µs duration, 0.4 duty-cycle, and average acoustic power varying from 2 W to 6 W. Ultrasound waves were generated by a flat circular piezoelectric transducer with a diameter of 25 mm. Cell viability was determined by MTT assay. Structural cellular changes were visualized with a fluorescence microscope. Signs of cytotoxicity such as a decrease in cell viability, chromatin condensation and apoptosis were found. ALA-mediated SDT evokes cytotoxic effects of low intensity US on rat RG2 glioma cells in vitro . This cell line is indicated for further preclinical assessment of SDT in in vivo conditions.

  6. COL Application Content Guide for HTGRs: Revision to RG 1.206, Part 1 - Status Report

    Energy Technology Data Exchange (ETDEWEB)

    Wayne Moe

    2012-08-01

    A combined license (COL) application is required by the Nuclear Regulatory Commission (NRC) for all proposed nuclear plants. The information requirements for a COL application are set forth in 10 CFR 52.79, “Contents of Applications; Technical Information in Final Safety Analysis Report.” An applicant for a modular high temperature gas-cooled reactor (HTGR) must develop and submit for NRC review and approval a COL application which conforms to these requirements. The technical information necessary to allow NRC staff to evaluate a COL application and resolve all safety issues related to a proposed nuclear plant is detailed and comprehensive. To this, Regulatory Guide (RG) 1.206, “Combined License Applications for Nuclear Power Plants” (LWR Edition), was developed to assist light water reactor (LWR) applicants in incorporating and effectively formatting required information for COL application review (Ref. 1). However, the guidance prescribed in RG 1.206 presumes a LWR design proposal consistent with the systems and functions associated with large LWR power plants currently operating under NRC license.

  7. WD+RG systems as the progenitors of type Ia supernovae

    International Nuclear Information System (INIS)

    Wang Bo; Han Zhanwen

    2010-01-01

    Type Ia supernovae (SNe Ia) play an important role in the study of cosmic evolution, especially in cosmology. There are several progenitor models for SNe Ia proposed in the past years. By considering the effect of accretion disk instability on the evolution of white dwarf (WD) binaries, we performed detailed binary evolution calculations for the WD + red-giant (RG) channel of SNe Ia, in which a carbon-oxygen WD accretes material from a RG star to increase its mass to the Chandrasekhar mass limit. According to these calculations, we mapped out the initial and final parameters for SNe Ia in the orbital period-secondary mass (log P i - M i 2 ) plane for various WD masses for this channel. We discussed the influence of the variation of the duty cycle value on the regions for producing SNe Ia. Similar to previous studies, this work also indicates that the long-period dwarf novae offer possible ways for producing SNe Ia. Meanwhile, we find that the surviving companion stars from this channel have a low mass after the SN explosion, which may provide a means for the formation of the population of single low-mass WDs ( o-dot ).

  8. Morfoanatomia dos órgãos vegetativos de Piper hispidum Sw. (Piperaceae

    Directory of Open Access Journals (Sweden)

    Adriana L.M. Albiero

    Full Text Available O presente trabalho teve por objetivo o estudo morfoanatômico dos órgãos vegetativos de Piper hispidum, visando a estabelecer características marcantes para a sua identificação e auxiliar estudos taxonômicos e farmacobotânicos. O material vegetal fresco e fixado foi estudado segundo as técnicas usuais de corte e coloração, incluindo análise em MEV. Piper hispidum é um arbusto com caule cilíndrico, nodoso, verde claro, com folhas alternas, ovadas, de cor verde-escura na face adaxial e verde claro na abaxial. Dentre as características anatômicas importantes para sua identificação destacam-se: parênquima cortical da raiz apresentando grupos de esclereídes. Córtex caulinar com faixas descontínuas de colênquima do tipo angular e tecido vascular organizado em dois círculos descontínuos de feixes colaterais. A folha é dorsiventral, hipoestomática, com estômatos tetracíticos. Hipoderme adaxial descontínua e abaxial frouxa com número variável de camadas; tricomas tectores e glandulares ocorrem nas duas faces. Epiderme uniestratificada e idioblastos oleíferos ocorrem em todos os órgãos.

  9. The Amelioration of N-Acetyl-p-Benzoquinone Imine Toxicity by Ginsenoside Rg3: The Role of Nrf2-Mediated Detoxification and Mrp1/Mrp3 Transports

    Directory of Open Access Journals (Sweden)

    Sang Il Gum

    2013-01-01

    Full Text Available Previously, we found that Korean red ginseng suppressed acetaminophen (APAP-induced hepatotoxicity via alteration of its metabolic profile involving GSTA2 induction and that ginsenoside Rg3 was a major component of this gene induction. In the present study, therefore, we assessed the protective effect of Rg3 against N-acetyl-p-benzoquinone imine (NAPQI, a toxic metabolic intermediate of APAP. Excess NAPQI resulted in GSH depletion with increases in the ALT and AST activities in H4IIE cells. Rg3 pretreatment reversed GSH depletion by NAPQI. Rg3 resulted in increased mRNA levels of the catalytic and modulatory subunit of glutamate cysteine ligase (GCL, the rate-limiting steps in GSH synthesis and subsequently increased GSH content. Rg3 increased levels of nuclear Nrf2, an essential transcriptional factor of these genes. The knockdown or knockout of the Nrf2 gene abrogated the inductions of mRNA and protein by Rg3. Abolishment of the reversal of GSH depletion by Rg3 against NAPQI was observed in Nrf2-deficient cells. Rg3 induced multidrug resistance-associated protein (Mrp 1 and Mrp3 mRNA levels, but not in Nrf2-deficient cells. Taken together, these results demonstrate that Rg3 is efficacious in protecting hepatocytes against NAPQI insult, due to GSH repletion and coordinated gene regulations of GSH synthesis and Mrp family genes by Nrf2.

  10. Síndrome pulmonar por hantavírus com disfunção de múltiplos órgãos: relato de caso Hantavirus pulmonary syndrome with multiple organ dysfunctions: case report

    Directory of Open Access Journals (Sweden)

    Marcelo Spegiorin Moreno

    2007-12-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A hantavirose é uma zoonose que apresenta distribuição mundial e sua transmissão está relacionada com o íntimo contato com roedores. Causa dois tipos de doença: a febre hemorrágica com síndrome renal (FHSR, endêmica na Ásia e Europa e a síndrome pulmonar por hantavírus (SPH, encontrada no continente americano, inclusive no Brasil, com elevadas taxas de mortalidade. O objetivo deste estudo foi relatar um caso de SPH com disfunção de múltiplos órgãos, que recebeu tratamento intensivo precoce e reanimação guiada por parâmetros de fluxo e de perfusão tecidual. RELATO DO CASO: Paciente do sexo masculino, 36 anos, iniciou quadro febril inespecífico, dispnéia progressiva, hipóxia grave e insuficiência respiratória aguda. Apresentava infiltrado interstício-alveolar difuso na radiografia de tórax. Evoluiu com disfunção de múltiplos órgãos (pulmonar, renal, hematológica, cardiovascular e metabólica. Recebeu tratamento e monitorização hemodinâmica invasiva precoces. As alterações laboratoriais mais importantes foram plaquetopenia, elevação dos níveis de hematócrito e hemoglobina, leucocitose, elevação de transaminases, de lactado desidrogenase e sorologia positiva para hantavírus (ELISA IgM positivo. O paciente apresentou reversão das disfunções orgânicas, recebendo alta hospitalar após 21 dias de hospitalização. CONCLUSÕES: A reanimação precoce e agressiva dirigida por metas levou à reversão da síndrome de falência de múltiplos órgãos e a um desfecho clínico favorável, apesar da gravidade da doença.BACKGROUND AND OBJECTIVES: Hantavirus infection is a zoonose with worldwide distribution. The transmission is related to the intimal contact with rodents. It causes two syndromes: hemorrhagic fever with renal syndrome (HFRS, endemic in Asia and Europe and the Hantavirus pulmonary syndrome (HPS, found in the American continent, including Brazil, with high mortality

  11. Successful rotational atherectomy over RG3 guidewire after failure of various techniques to deliver RotaWire.

    Science.gov (United States)

    Kaneko, Umihiko; Kashima, Yoshifumi; Kanno, Daitaro; Sugie, Takuro; Kobayashi, Ken; Fujita, Tsutomu

    2017-10-01

    Although performing rotational atherectomy (RA) requires guidewire exchange for the dedicated guidewire, RotaWire guidewire (Boston Scientific) exhibits much lower performance than conventional guidewire. Consequently, there are times when RotaWire cannot be advanced past the lesion independently or using a microcatheter exchange technique, rendering RA impossible. We present a case of a heavily calcified, device-uncrossable, and non-expansible chronic total occlusion lesion successfully revascularized with RA over RG3 guidewire (Asahi Intecc), which has a length of 330 cm, hydrophilic coating, and a 0.010-inch-long shaft. RG3 provided excellent cross-ability and RA could also be performed over RG3 without guidewire exchange for the RotaWire.

  12. Inhibitory effect of ginsenoside Rg3 combined with gemcitabine on angiogenesis and growth of lung cancer in mice

    International Nuclear Information System (INIS)

    Liu, Tai-Guo; Huang, Ying; Cui, Dan-Dan; Huang, Xiao-Bing; Mao, Shu-Hua; Ji, Ling-Ling; Song, Hai-Bo; Yi, Cheng

    2009-01-01

    Ginsenoside Rg3, a saponin extracted from ginseng, inhibits angiogenesis. The combination of low-dose chemotherapy and anti-angiogenic inhibitors suppresses growth of experimental tumors more effectively than conventional therapy or anti-angiogenic agent alone. The present study was designed to evaluate the efficacy of low-dose gemcitabine combined with ginsenoside Rg3 on angiogenesis and growth of established Lewis lung carcinoma in mice. C57L/6 mice implanted with Lewis lung carcinoma were randomized into the control, ginsenoside Rg3, gemcitabine and combination group. The quality of life and survival of mice were recorded. Tumor volume, inhibitive rate and necrosis rate were estimated. Necrosis of tumor and signals of blood flow as well as dynamic parameters of arterial blood flow in tumors such as peak systolic velocity (PSV) and resistive index (RI) were detected by color Doppler ultrasound. In addition, expression of vascular endothelial cell growth factor (VEGF) and CD31 were observed by immunohistochemstry, and microvessel density (MVD) of the tumor tissues was assessed by CD31 immunohistochemical analysis. Quality of life of mice in the ginsenoside Rg3 and combination group were better than in the control and gemcitabine group. Combined therapy with ginsenoside Rg3 and gemcitabine not only enhanced efficacy on suppression of tumor growth and prolongation of the survival, but also increased necrosis rate of tumor significantly. In addition, the combination treatment could obviously decrease VEGF expression and MVD as well as signals of blood flow and PSV in tumors. Ginsenoside Rg3 combined with gemcitabine may significantly inhibit angiogenesis and growth of lung cancer and improve survival and quality of life of tumor-bearing mice. The combination of chemotherapy and anti-angiogenic drugs may be an innovative and promising therapeutic strategy in the experimental treatment of human lung cancer

  13. Atividade biológica de metabólitos secundários de algas marinhas do gênero Laurencia

    Directory of Open Access Journals (Sweden)

    Fernanda L. da S. Machado

    Full Text Available As algas vermelhas do gênero Laurencia são conhecidas como uma riquíssima fonte de metabólitos secundários. As principais classes químicas já isoladas são terpenos - sesquiterpenos, diterpenos, triterpenos - e acetogeninas. Estudos apontam que estas substâncias apresentam importantes atividades biológicas, principalmente citotóxica e antibacteriana. Existem ainda alguns poucos trabalhos com informações sobre atividade antiparasitária, antiviral e antifúngica dos metabólitos de Laurencia. O forte perfil bioativo observado em alguns estudos sugere que alguns destes metabólitos podem ser futuramente utilizados como fármacos ou como protótipos de novos agentes antitumorais e antibacterianos. Muitos dos metabólitos de Laurencia ainda não foram avaliados sob o ponto de vista farmacológico, o que representa um grande potencial a ser explorado por diversos campos da farmacologia.

  14. Control glucémico y metabólico integral: dos metas complementarias para el paciente diabético

    Directory of Open Access Journals (Sweden)

    Alicia Alayón

    2008-01-01

    Conclusión: Es insuficiente la medida aislada de HbA1c como evaluadora del estado metabólico integral del paciente diabético. Es necesario fortalecer las estrategias para reducir el riesgo cardiovascular en esta población.

  15. Función endotelial y lipemia postprandial en adultos con presencia de criterios asociados a síndrome metabólico: efecto del estado nutricional

    Directory of Open Access Journals (Sweden)

    Diana Milena Díaz-Vidal

    2017-07-01

    Conclusión: Este estudio demuestra que los sujetos clasificados en sobrepeso y que presentan criterios asociados al síndrome metabólico, muestran un perfil cardiometabólico asociado con un mayor riesgo cardiovascular, tras una ingesta alta en grasas.

  16. PPAR agonists reduce steatosis in oleic acid-overloaded HepaRG cells

    International Nuclear Information System (INIS)

    Rogue, Alexandra; Anthérieu, Sébastien; Vluggens, Aurore; Umbdenstock, Thierry; Claude, Nancy; Moureyre-Spire, Catherine de la; Weaver, Richard J.; Guillouzo, André

    2014-01-01

    Although non-alcoholic fatty liver disease (NAFLD) is currently the most common form of chronic liver disease there is no pharmacological agent approved for its treatment. Since peroxisome proliferator-activated receptors (PPARs) are closely associated with hepatic lipid metabolism, they seem to play important roles in NAFLD. However, the effects of PPAR agonists on steatosis that is a common pathology associated with NAFLD, remain largely controversial. In this study, the effects of various PPAR agonists, i.e. fenofibrate, bezafibrate, troglitazone, rosiglitazone, muraglitazar and tesaglitazar on oleic acid-induced steatotic HepaRG cells were investigated after a single 24-hour or 2-week repeat treatment. Lipid vesicles stained by Oil-Red O and triglycerides accumulation caused by oleic acid overload, were decreased, by up to 50%, while fatty acid oxidation was induced after 2-week co-treatment with PPAR agonists. The greatest effects on reduction of steatosis were obtained with the dual PPARα/γ agonist muraglitazar. Such improvement of steatosis was associated with up-regulation of genes related to fatty acid oxidation activity and down-regulation of many genes involved in lipogenesis. Moreover, modulation of expression of some nuclear receptor genes, such as FXR, LXRα and CAR, which are potent actors in the control of lipogenesis, was observed and might explain repression of de novo lipogenesis. Conclusion: Altogether, our in vitro data on steatotic HepaRG cells treated with PPAR agonists correlated well with clinical investigations, bringing a proof of concept that drug-induced reversal of steatosis in human can be evaluated in in vitro before conducting long-term and costly in vivo studies in animals and patients. - Highlights: • There is no pharmacological agent approved for the treatment of NAFLD. • This study demonstrates that PPAR agonists can reduce fatty acid-induced steatosis. • Some nuclear receptors appear to be potent actors in the control

  17. PPAR agonists reduce steatosis in oleic acid-overloaded HepaRG cells

    Energy Technology Data Exchange (ETDEWEB)

    Rogue, Alexandra [Inserm UMR 991, 35043 Rennes Cedex (France); Université de Rennes 1, Faculté des Sciences Pharmaceutiques et Biologiques, 35043 Rennes Cedex (France); Biologie Servier, Gidy (France); Anthérieu, Sébastien; Vluggens, Aurore [Inserm UMR 991, 35043 Rennes Cedex (France); Université de Rennes 1, Faculté des Sciences Pharmaceutiques et Biologiques, 35043 Rennes Cedex (France); Umbdenstock, Thierry [Technologie Servier, Orléans (France); Claude, Nancy [Institut de Recherches Servier, Courbevoie (France); Moureyre-Spire, Catherine de la; Weaver, Richard J. [Biologie Servier, Gidy (France); Guillouzo, André, E-mail: Andre.Guillouzo@univ-rennes1.fr [Inserm UMR 991, 35043 Rennes Cedex (France); Université de Rennes 1, Faculté des Sciences Pharmaceutiques et Biologiques, 35043 Rennes Cedex (France)

    2014-04-01

    Although non-alcoholic fatty liver disease (NAFLD) is currently the most common form of chronic liver disease there is no pharmacological agent approved for its treatment. Since peroxisome proliferator-activated receptors (PPARs) are closely associated with hepatic lipid metabolism, they seem to play important roles in NAFLD. However, the effects of PPAR agonists on steatosis that is a common pathology associated with NAFLD, remain largely controversial. In this study, the effects of various PPAR agonists, i.e. fenofibrate, bezafibrate, troglitazone, rosiglitazone, muraglitazar and tesaglitazar on oleic acid-induced steatotic HepaRG cells were investigated after a single 24-hour or 2-week repeat treatment. Lipid vesicles stained by Oil-Red O and triglycerides accumulation caused by oleic acid overload, were decreased, by up to 50%, while fatty acid oxidation was induced after 2-week co-treatment with PPAR agonists. The greatest effects on reduction of steatosis were obtained with the dual PPARα/γ agonist muraglitazar. Such improvement of steatosis was associated with up-regulation of genes related to fatty acid oxidation activity and down-regulation of many genes involved in lipogenesis. Moreover, modulation of expression of some nuclear receptor genes, such as FXR, LXRα and CAR, which are potent actors in the control of lipogenesis, was observed and might explain repression of de novo lipogenesis. Conclusion: Altogether, our in vitro data on steatotic HepaRG cells treated with PPAR agonists correlated well with clinical investigations, bringing a proof of concept that drug-induced reversal of steatosis in human can be evaluated in in vitro before conducting long-term and costly in vivo studies in animals and patients. - Highlights: • There is no pharmacological agent approved for the treatment of NAFLD. • This study demonstrates that PPAR agonists can reduce fatty acid-induced steatosis. • Some nuclear receptors appear to be potent actors in the control

  18. Validação de equações de predição da taxa metabólica basal em mulheres residentes em Porto Alegre, RS, Brasil

    Directory of Open Access Journals (Sweden)

    Wahrlich Vivian

    2001-01-01

    Full Text Available OBJETIVO: Medir a taxa metabólica basal em mulheres de 20 a 40 anos, não-gestantes ou lactantes, e comparar o valor medido com os valores de taxa metabólica basal estimados por equações de predição. MÉTODOS: A taxa metabólica basal foi medida por calorimetria indireta, pela manhã, durante a fase folicular do ciclo menstrual, em 60 voluntárias residentes no município de Porto Alegre, RS, sob condições padronizadas de jejum, repouso e ambiente. RESULTADOS: A média (± desvio-padrão da taxa metabólica basal medida foi 1.185,3±148,6 kcal em 24 horas. A taxa metabólica basal, estimada por equações, foi significativamente maior (7% a 17% do que a taxa metabólica basal medida. CONCLUSÕES: Os dados evidenciaram que as equações de predição não são adequadas para estimar a taxa metabólica basal nas mulheres avaliadas. O emprego dessas equações podem superestimar os requerimentos energéticos para mulheres com características semelhantes.

  19. Validação de equações de predição da taxa metabólica basal em mulheres residentes em Porto Alegre, RS, Brasil

    Directory of Open Access Journals (Sweden)

    Vivian Wahrlich

    2001-02-01

    Full Text Available OBJETIVO: Medir a taxa metabólica basal em mulheres de 20 a 40 anos, não-gestantes ou lactantes, e comparar o valor medido com os valores de taxa metabólica basal estimados por equações de predição. MÉTODOS: A taxa metabólica basal foi medida por calorimetria indireta, pela manhã, durante a fase folicular do ciclo menstrual, em 60 voluntárias residentes no município de Porto Alegre, RS, sob condições padronizadas de jejum, repouso e ambiente. RESULTADOS: A média (± desvio-padrão da taxa metabólica basal medida foi 1.185,3±148,6 kcal em 24 horas. A taxa metabólica basal, estimada por equações, foi significativamente maior (7% a 17% do que a taxa metabólica basal medida. CONCLUSÕES: Os dados evidenciaram que as equações de predição não são adequadas para estimar a taxa metabólica basal nas mulheres avaliadas. O emprego dessas equações podem superestimar os requerimentos energéticos para mulheres com características semelhantes.

  20. Alterações metabólicas e inflamatórias na gestação

    Directory of Open Access Journals (Sweden)

    Josilaine Tonin Ribas

    2015-10-01

    Full Text Available Em gestantes, ocorrem alterações hormonais, metabólicas e hematológicas, fisiologicamente importantes. As alterações metabólicas observadas estão associadas ao aumento do efeito hepático da progesterona, estradiol e lactogênio placentário nesse período. Estrogênios são conhecidos por aumentar a produção de colesterol VLDL e diminuir a atividade da lipase hepática. Nesse grupo de mulheres, notase também um aumento do número de neutrófilos e monócitos para a circulação periférica, notadamente em gestantes com pré-eclampsia, tendo como consequência produção e secreção da mieloperoxidase. Assim, torna-se importante determinar quais alterações metabólicas e inflamatórias estão relacionadas com a gestação, para auxiliar no diagnóstico e prognóstico de doenças cardiovasculares futuras nesta população. Além dos fatores de risco, esta revisão aponta novos biomarcadores, tais como a mieloperoxidase, capacidade antioxidante total do soro, marcadores inflamatórios e proteína plasmática A.Palavras-chave: Gestação. Biomarcadores. Metabolismo. Inflamação. ABSTRACT In pregnant women, hormonal, metabolic and hematologic physiologically important changes occur. The metabolic changes observed are associated with increased hepatic effect of estradiol, progesterone and placental lactogen during this period. Estrogens are known to increase production of VLDL cholesterol and decrease the activity of hepatic lipase. In this group of women, we can note an increased number of neutrophils and monocytes into the peripheral circulation, especially in pregnant women with pre-eclampsia, resulting in production and secretion of myeloperoxidase. Thus, it becomes important to determine which metabolic and inflammatory changes are related to pregnancy, to assist in the diagnosis and prognosis of future cardiovascular disease in this population. In addition to the risk factors, this review shows new biomarkers such as

  1. Síndrome metabólico en un área de salud de Cienfuegos. Segunda medición de CARMEN

    Directory of Open Access Journals (Sweden)

    Alain Francisco Morejón Giraldoni

    2011-02-01

    Full Text Available Fundamento: El conocimiento de las prevalencias de los principales factores de riesgos cardiovasculares y del síndrome metabólico en una comunidad, es determinante para establecer programas preventivos de salud.Objetivo: Determinar la prevalencia del síndrome metabólico en un área de salud de la ciudad de Cienfuegos, así como su relación con los principales factores de riesgo.Métodos: Se realizó un estudio de corte transversal del tipo descriptivo correlacional. La muestra se obtuvo mediante un método probabilístico y es representativa de la población mayor de 17 años del área de salud IV del municipio de Cienfuegos; se estudiaron 209 personas extraídas de un universo de 16577 individuos, se utilizó un porcentaje de prevalencia esperada del 18 %, un intervalo de confianza (IC del 95 %, una precisión de ± 5 % y una tasa de no respuesta del 35 %.Resultados: La prevalencia de síndrome metabólico se determinó en 21,7 %, la prevalencia de hipertensión arterial fue de 39,2 %, la obesidad en 24,8 % y la diabetes mellitus en 10,7 %. La prevalencia de síndrome metabólico en mayores de 40 años fue de 43,7 % con una razón de prevalencia de 2,40 para el sexo masculino.Conclusiones: Existe una alta prevalencia de los principales factores de riesgo en la comunidad, la principal triada de asociación para el síndrome metabólico esta formada por la hipertensión, hipertrigliceridemia e hipocolesterolemia de HDL.

  2. Decoupling in an expanding universe boundary RG-flow affects initial conditions for inflation

    CERN Document Server

    Schalm, K; Van der Schaar, J P; Schalm, Koenraad; Shiu, Gary; Schaar, Jan Pieter van der

    2004-01-01

    We study decoupling in FRW spacetimes, emphasizing a Lagrangian description throughout. To account for the vacuum choice ambiguity in cosmological settings, we introduce an arbitrary boundary action representing the initial conditions. RG flow in these spacetimes naturally affects the boundary interactions. As a consequence the boundary conditions are sensitive to high-energy physics through irrelevant terms in the boundary action. Using scalar field theory as an example, we derive the leading dimension four irrelevant boundary operators. We discuss how the known vacuum choices, e.g. the Bunch-Davies vacuum, appear in the Lagrangian description and square with decoupling. For all choices of boundary conditions encoded by relevant boundary operators, of which the known ones are a subset, backreaction is under control. All, moreover, will generically feel the influence of high-energy physics through irrelevant (dimension four) boundary corrections. Having established a coherent effective field theory framework ...

  3. Historical thinking in clinical medicine: lessons from R.G. Collingwood's philosophy of history.

    Science.gov (United States)

    Chin-Yee, Benjamin H; Upshur, Ross E G

    2015-06-01

    The aim of this article is to create a space for historical thinking in medical practice. To this end, we draw on the ideas of R.G. Collingwood (1889-1943), the renowned British philosopher of history, and explore the implications of his philosophy for clinical medicine. We show how Collingwood's philosophy provides a compelling argument for the re-centring of medical practice around the patient history as a means of restoring to the clinical encounter the human meaning that is too often lost in modern medicine. Furthermore, we examine how Collingwood's historical thinking offers a patient-centred epistemology and a more pluralistic concept of evidence that includes the qualitative, narrative evidence necessary for human understanding. We suggest that clinical medicine can benefit from Collingwood's historical thinking, and, more generally, illustrates how a philosophy of medicine that draws on diverse sources from the humanities offers a richer, more empathetic clinical practice. © 2015 John Wiley & Sons, Ltd.

  4. Life-finding detector development at NASA GSFC using a custom H4RG test bed

    Science.gov (United States)

    Mosby, Gregory; Rauscher, Bernard; Kutyrev, Alexander

    2018-01-01

    Chemical species associated with life, called biosignatures, should be visible in exoplanet atmospheres with larger space telescopes. These signals will be faint and require very low noise (~e-) detectors to robustly measure. At NASA Goddard we are developing a single detector H4RG test bed to characterize and identify potential technology developments needed for the next generation's large space telescopes. The vacuum and cryogenic test bed will include near infrared light sources from integrating spheres using a motorized shutter. The detector control and readout will be handled by a Leach controller. Detector cables have been manufactured and test planning has begun. Planned tests include testing minimum read noise capabilities, persistence mitigation strategies using long wavelength light, and measuring intrapixel variation which might affect science goals of future missions. In addition to providing a means to identify areas of improvement in detector technology, we hope to use this test bed to probe some fundamental physics of these infrared arrays.

  5. Whole-genome sequence of Pseudomonas fluorescens EK007-RG4, a promising biocontrol agent against a broad range of bacteria, including the fire blight bacterium Erwinia amylovora

    DEFF Research Database (Denmark)

    Habibi, Roghayeh; Tarighi, Saeed; Behravan, Javad

    2017-01-01

    Here, we report the first draft whole-genome sequence of Pseudomonas fluorescens strain EK007-RG4, which was isolated from the phylloplane of a pear tree. P. fluorescens EK007-RG4 displays strong antagonism against Erwinia amylovora, the causal agent for fire blight disease, in addition to several...

  6. Ginsenoside Rg1 Ameliorates Behavioral Abnormalities and Modulates the Hippocampal Proteomic Change in Triple Transgenic Mice of Alzheimer’s Disease

    Directory of Open Access Journals (Sweden)

    Lulin Nie

    2017-01-01

    Full Text Available Alzheimer’s disease (AD is one of the most common neurodegenerative diseases, so far, there are no effective measures to prevent and cure this deadly condition. Ginsenoside Rg1 (Rg1 was shown to improve behavioral abnormalities in AD; however, the potential mechanisms remain unclear. In this study, we pretreated 7-month-old 3xTg-AD mice for 6 weeks with Rg1 and evaluated the effects of Rg1 on the behaviors and the protein expression of hippocampal tissues. The behavioral tests showed that Rg1 could improve the memory impairment and ameliorate the depression-like behaviors of 3xTg-AD mice. Proteomic results revealed a total of 28 differentially expressed hippocampal proteins between Rg1-treated and nontreated 3xTg-AD mice. Among these proteins, complexin-2 (CPLX2, synapsin-2 (SYN2, and synaptosomal-associated protein 25 (SNP25 were significantly downregulated in the hippocampus of 3xTg-AD mice compared with the WT mice, and the treatment of Rg1 modulated the expression of CPLX2 and SNP25 in the hippocampus of 3xTg-AD mice. The expression of CPLX2, SYN2, and SNP25 was further validated by Western blot analysis. Taken together, we concluded that Rg1 could be a potential candidate drug to improve the behavioral deficits in AD via modulating the expression of the proteins (i.e., CPLX2, SYN2, and SNP25.

  7. Effects of curcumin-loaded PLGA nanoparticles on the RG2 rat glioma model.

    Science.gov (United States)

    Orunoğlu, Merdan; Kaffashi, Abbas; Pehlivan, Sibel Bozdağ; Şahin, Selma; Söylemezoğlu, Figen; Oğuz, Kader Karli; Mut, Melike

    2017-09-01

    Curcumin, the active ingredient of turmeric, has a remarkable antitumor activity against various cancers, including glioblastoma. However, it has poor absorption and low bioavailability; thus, to cross the blood-brain barrier and reach tumor tissue, it needs to be transferred to tumor site by special drug delivery systems, such as nanoparticles. We aimed to evaluate the antitumor activity of curcumin on glioblastoma tissue in the rat glioma-2 (RG2) tumor model when it is loaded on poly(lactic-co-glycolic acid)-1,2-distearoyl-glycerol-3-phospho-ethanolamine-N-[methoxy (polyethylene glycol)-2000] ammonium salt (PLGA-DSPE-PEG) hybrid nanoparticles. Glioblastoma was induced in 42 adult female Wistar rats (250-300g) by RG2 tumor model. The curcumin-loaded nanoparticles were injected by intravenous (n=6) or intratumoral route (n=6). There were five control groups, each containing six rats. First control group was not applied any treatment. The remaining four control groups were given empty nanoparticles or curcumin alone by intravenous or intratumoral route, respectively. The change in tumor volume was assessed by magnetic resonance imaging and histopathology before and 5days after drug injections. Tumor size decreased significantly after 5days of intratumoral injection of curcumin-loaded nanoparticle (from 66.6±44.6 to 34.9±21.7mm 3 , p=0.028), whereas it significantly increased in nontreated control group (from 33.9±21.3 to 123.7±41.1mm 3 , p=0.036) and did not significantly change in other groups (p>0.05 for all). In this in vivo experimental model, intratumoral administration of curcumin-loaded PLGA-DSPE-PEG hybrid nanoparticles was effective against glioblastoma. Curcumine-loaded nanoparticles may have potential application in chemotherapy of glioblastoma. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Efectividad del Programa Educativo “Vida Saludable” en el Cambio de Estilos de Vida de Pacientes con Síndrome Metabólico. Hospital I Cono Sur Essalud - Nuevo Chimbote 2015.

    OpenAIRE

    Rondán Caballero, Nancy Verónica

    2016-01-01

    El presente estudio de investigación es cuasi-experimental con objetivo Conocer la efectividad del programa educativo “Vida Saludable” en el Cambio de Estilos de Vida de Pacientes con Síndrome Metabólico. Hospital I Cono Sur Essalud - Nuevo Chimbote 2015.Sustentado en Teoría General de Sistemas de Bertalanffy. Muestra 50 pacientes con Síndrome Metabólico, se aplicó un test, un programa educativo y post-test, concluyendo:  El estilo de vida de los pacientes con síndrome metabólico del Hosp...

  9. Expressão hormonal e metabólica dos carcinomas broncogénicos

    OpenAIRE

    Cyro Teixeira da Silva Junior; Gilberto Perez Cardoso

    2003-01-01

    RESUMO: No presente artigo de revisão os autores abordam um tema não levado em consideração pela maioria dos clínicos e pneumologistas, que são as manifestações endócrino-metabólicas do carcinoma broncogénico. As principais manifestações encontradas na literatura são as síndromas para neoplásicas (síndroma de secreção inapropriada da hormona antidiurética, alterações no metabolismo do cálcio, síndroma carcinóide e de Cushing). A osteoartropatia hipertrófica também Ã...

  10. Síndrome metabólico y su relación con hipogonadismo tardío

    Directory of Open Access Journals (Sweden)

    Dra. A. Verónica Araya Q.

    2014-01-01

    Conclusiones: En todo paciente con un hipogonadismo demostrado se debe buscar elementos del SM pero, en los pacientes con SM o DM2, la medición de T se debe considerar sólo si hay otros elementos clínicos que orienten a una deficiencia de andrógenos. En cuanto a la indicación de terapia de reemplazo en los pacientes diabéticos o con SM, aún faltan estudios controlados, a largo plazo que evalúen los beneficios reales, metabólicos y cardiovasculares. Lo fundamental en estos pacientes es la prevención, promoviendo un estilo de vida saludable.

  11. Perspectivas moleculares y metabólicas de la suplementación con creatina en el entrenamiento de fuerza

    Directory of Open Access Journals (Sweden)

    Diego A. Bonilla

    2015-01-01

    Full Text Available En los últimos años el problema de investigación en el campo de la suplementación deportiva ha cambiado al punto de explicar los mecanismos metabólicos por los cuales la administración de creatina (Cr incrementa el rendimiento en ciertos deportes o simplemente beneficia la adaptación muscular. Esta revisión analiza por primera vez los mecanismos bioquímicos de la ingesta de Cr desde la perspectiva de señalización celular, enfocándose en la mayor biodisponibilidad energética de Cr y optimización de la acción buffer espacial/ temporal que ofrece el sistema Cr/PCr/CK. Además, se examinan aspectos relacionados con el incremento en los procesos de proliferación y diferenciación de células musculares (IGF-I/PI3K/Akt-PKB, SPHK1/ MAPK/p38/MRFs, mTOR, hinchamiento celular, actividad mitótica de células satélite, polimerización de actina y fusión de mioblastos y la inactivación y/o reducción en la expresión de proteínas con funciones ergolíticas (GSK3β, miostatina y regulación de AMPK. De esta manera, se explican el aumento de la masa muscular, la fuerza, la resistencia a la fatiga y el rendimiento en ejercicios de alta intensidad, producidos por la suplementación con monohidrato de Cr, desde un punto de vista metabólico.

  12. Eficacia educacional en control metabólico de diabéticos con diálisis peritoneal

    Directory of Open Access Journals (Sweden)

    María Adelaida Zapata-Zapata

    2017-01-01

    Full Text Available La segunda causa de enfermedad renal crónica mundial es la diabetes mellitus, con repercusiones individuales y socioeconómicas. La hemoglobina glucosilada (HbA1c es un marcador del control metabólico; su reducción se ha asociado a disminución en complicaciones microvasculares y neuropáticas de la diabetes. Objetivo: determinar la eficacia de un programa educativo a pacientes diabéticos en diálisis peritoneal según niveles de Hb1Ac como parámetro de control metabólico en una unidad renal de Cali, Colombia. Metodología: estudio cuasi experimental de junio 2013 a febrero de 2014 con 150 sujetos diabéticos tipo 2 en diálisis peritoneal asignados a 3 grupos, según análisis X2 , t de Student, ANOVA, ANCOVA y regresión lineal múltiple, con IBM-SPSS®. Resultados: las características sociodemográficas y clínicas de los 3 grupos no presentaron diferencias significativas en línea base. Se observó diferencia significativa en el conocimiento post intervención del grupo intervenido con cada módulo (p<0,05. Niveles de Hb1Ac sin diferencias estadísticamente significativas a 3 meses. Hubo diferencia a 6 meses de intervención entre el grupo intervenido respecto de los 2 de control (p<0,05. Conclusiones: la intervención educativa puede ayudar a disminuir niveles de Hb1Ac en el paciente diabético con diálisis peritoneal, siempre que la intervención sea continua.

  13. Manifestações endócrino-metabólicas na tuberculose

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    Cyro Teixeira da Silva Junior

    2003-07-01

    Full Text Available RESUMO: No presente artigo de revisão os autores abordam um tema não levado em consideração pela maioria dos clínicos e pneumologistas, que são as manifestações endócrino-metabólicas da tuberculose. As principais manifestações encon tradas na literatura são: insuficiência adrenal, síndroma de secreção inapropriada da hormona antidiurética, alterações no metabolismo do cálcio, hipopituitarismo, tireoidopatias, alterações pancreáticas, manifestações da tuberculose nos diabéticos e as alterações metabólicas produzidas pelos tuberculostáticos.Chama a atenção na discussão realizada a quantidade de informações sobre as alterações no metabolismo do cálcio.REV PORT PNEUMOL 2003; IX (4: 353-358 ABSTRACT: In the present paper, the authors reviews the endocrine and metabolic manifestations in tuberculosis. Disorders as adrenal insufficiency, the syndrome of inappropriate antidiuretic hormone secretion (SIADH, hypercalcemia, endocrine effects of antituberculous drugs, hypopituitarism, tuberculous involvement of the thyroid gland and pancreas and chest radiograph presentation in diabetes mellitus are discussed.In the literature, several reports support the presence of abnormal calcium metabolism in tuberculosis.REV PORT PNEUMOL 2003; IX (4: 353-358 Palavras-chave: tuberculose, metabolismo, efeitos endócrinos, Key-words: tuberculosis, metabolism, endocrine effects

  14. 78 FR 24803 - Hilco SP Rail, LLC-Acquisition and Operation Exemption-RG Steel Railroad Holding, LLC

    Science.gov (United States)

    2013-04-26

    ... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35734] Hilco SP Rail, LLC--Acquisition and Operation Exemption--RG Steel Railroad Holding, LLC Hilco SP Rail, LLC (Hilco), a noncarrier... Holding, LLC, and operate as a common carrier over an approximately 12-mile line of railroad in Sparrows...

  15. [Effect of ginsenoside Rg3 on Pim-3 and Bad proteins in human pancreatic cancer cell line PANC-1].

    Science.gov (United States)

    Jian, Jie; Hu, Zhi-Fang; Huang, Yuan

    2009-05-01

    Ginsenoside Rg3 is a traditional Chinese medicine monomer which possesses anticancer effects. This study was to investigate the effects of ginsenoside Rg3 on Pim-3 and phosphorylated Bad (pBad) proteins, pBad (Ser112) and pBad (Ser136) in human pancreatic cancer cell line PANC-1. PANC-1 cells were exposed to 10, 20, 40 and 80 micromol/L ginsenoside Rg3 for 24 h. A short hairpin RNA (shRNA) of Pim-3 was cloned and inserted into a eukaryotic expression vector pSilencer 3.1-H1 Neo to construct pSilencer 3.1-H1 Neo-Pim-3. pSilencer 3.1-H1 Neo-Pim-3 was then transfected into PANC-1 cells. Cell proliferation was measured by MTT assay; cell apoptosis was observed under an invert microscope and measured by flow cytometry with Annexin V/PI staining; protein expressions of Pim-3, Bad, pBad (Ser112) and pBad (Ser136) were measured by Western blot. The inhibitory rates of 10, 20, 40 and 80 micromol/L ginsenoside Rg3 on PANC-1 cells were 20.2%, 33.4%, 52.8% and 65.3%, respectively. Typical morphological changes in apoptosis were induced by ginsenoside Rg3. The apoptotic rate of PANC-1 cells was significantly higher in the ginsenoside Rg3 treatment group (80 micromol/L) than in the control group (12.2% vs. 3.3%, PPANC-1 cells. Compared with the control group, the percentages of early and total apoptotic cells were significantly increased in PANC-1 cells transfected with pim-3-shRNA [(11.5+/-3.7)% vs. (5.8+/-2.2)%,P<0.01;(20.8+/-2.6)% vs.(13.0+/-4.1)%,P<0.05], while the expressions of pim-3 and pBad (Ser112) were both decreased. The anti-tumor effect of ginsenoside Rg3 may be associated with the decrease of Pim-3 and pBad (Ser112).

  16. Preclinical efficacy of the MDM2 inhibitor RG7112 in MDM2 amplified and TP53 wild-type glioblastomas

    Science.gov (United States)

    Verreault, Maite; Schmitt, Charlotte; Goldwirt, Lauriane; Pelton, Kristine; Haidar, Samer; Levasseur, Camille; Guehennec, Jeremy; Knoff, David; Labussiere, Marianne; Marie, Yannick; Ligon, Azra H.; Mokhtari, Karima; Hoang-Xuan, Khe; Sanson, Marc; Alexander, Brian M; Wen, Patrick Y.; Delattre, Jean-Yves; Ligon, Keith L.; Idbaih, Ahmed

    2016-01-01

    Rationale p53 pathway alterations are key molecular events in glioblastoma (GBM). MDM2 inhibitors increase expression and stability of p53 and are presumed to be most efficacious in patients with TP53 wild-type and MDM2-amplified cancers. However, this biomarker hypothesis has not been tested in patients or patient-derived models for GBM. Methods We performed a preclinical evaluation of RG7112 MDM2 inhibitor, across a panel of 36 patient-derived GBM cell lines (PDCLs), each genetically characterized according to their P53 pathway status. We then performed a pharmacokinetic (PK) profiling of RG7112 distribution in mice and evaluated the therapeutic activity of RG7112 in orthotopic and subcutaneous GBM models. Results MDM2-amplified PDCLs were 44 times more sensitive than TP53 mutated lines that showed complete resistance at therapeutically attainable concentrations (avg. IC50 of 0.52 μM vs 21.9 μM). MDM4 amplified PDCLs were highly sensitive but showed intermediate response (avg. IC50 of 1.2 μM), whereas response was heterogeneous in TP53 wild-type PDCLs with normal MDM2/4 levels (avg. IC50 of 7.7 μM). In MDM2-amplified lines, RG7112 restored p53 activity inducing robust p21 expression and apoptosis. PK profiling of RG7112-treated PDCL intracranial xenografts demonstrated that the compound significantly crosses the blood-brain and the blood-tumor barriers. Most importantly, treatment of MDM2-amplified/TP53 wild-type PDCL-derived model (subcutaneous and orthotopic) reduced tumor growth, was cytotoxic, and significantly increased survival. Conclusion These data strongly support development of MDM2 inhibitors for clinical testing in MDM2-amplified GBM patients. Moreover, significant efficacy in a subset of non-MDM2 amplified models suggests that additional markers of response to MDM2 inhibitors must be identified. PMID:26482041

  17. Caracterización de niños y adolescentes obesos con síndrome metabólico

    Directory of Open Access Journals (Sweden)

    Yamileisy Santiago Martínez

    2012-03-01

    Full Text Available Introducción: el síndrome metabólico es más frecuente en niños y adolescentes con obesidad. Objetivo: determinar algunas características clínicas, antropométricas y de laboratorio en niños obesos con síndrome metabólico. Métodos: estudio transversal con 59 niños y adolescentes obesos con edades entre 2 y 14 años, seleccionados por muestreo aleatorio simple de 20 consultorios del municipio Urbano Noris, de Holguín, atendidos en abril-mayo de 2010. Las variables fueron: sexo, edad, peso, talla, índice de masa corporal, circunferencia abdominal, presión arterial, colesterol total, triglicéridos, glucemia, HDL-colesterol, LDL-colesterol, microalbuminuria y los índices colesterol total/HDL-colesterol y LDL-colesterol/HDL-colesterol, además, la presencia de hipertensión arterial y diabetes mellitus. Se empleó el análisis de varianza y la prueba c2 en SPSS y nivel de significación del 0,05. La pesquisa se realizó según los criterios cubanos basados en el Programa Nacional de Educación sobre el Colesterol de Estados Unidos. Resultados: la frecuencia del síndrome metabólico fue del 55,9 %. Estos pacientes presentaron valores superiores de la presión arterial, los triglicéridos, la glucemia, LDL-colesterol y de los índices aterogénicos y concentraciones más bajas de lipoproteínas de alta densidad, que el grupo sin síndrome metabólico (n= 26. Se encontró una asociación significativa entre el síndrome metabólico y la hipertensión arterial, no así con la microalbuminuria y la diabetes mellitus. No se encontraron diferencias de género. Conclusiones: es importante el diagnóstico del síndrome metabólico en niños y adolescentes para una mejor caracterización de este estado, y se recomienda una intervención basada en cambios de estilos de vida.

  18. Acidose metabólica e sua associação com o estado nutricional em hemodiálise

    Directory of Open Access Journals (Sweden)

    Claudia Maria Costa de Oliveira

    2015-12-01

    Full Text Available Resumo Introdução: A acidose metabólica é frequente em pacientes dialíticos e participa na patogênese da desnutrição energético-proteica destes pacientes. Objetivos: Avaliar a prevalência de acidose metabólica em hemodiálise e pesquisar sua associação com o estado nutricional. Métodos: Foi realizado um estudo transversal em pacientes em hemodiálise em um único centro. O estado nutricional foi avaliado por meio de indicadores antropométricos, bioquímicos e da bioimpedância elétrica multifrequencial. Acidose metabólica foi definida como bicarbonato sérico (BIC < 22 mEq/L, e os pacientes foram divididos em 3 grupos, segundo o BIC (< 15,15-21,9 e ≥ 22. A associação entre o BIC e as variáveis contínuas foi pesquisada com o teste de Kruskal Wallis. A correlação linear entre o BIC e as variáveis do estudo também foi testada. Resultados: Foram avaliados 95 pacientes, 59% masculinos, idade média 52,3 anos. A prevalência de acidose metabólica foi 94,7%. O IMC, o ganho de peso interdialítico e o PTH foram significativamente diferentes entre os 3 grupos de BIC. O BIC apresentou correlação negativa significativa com ureia, fósforo e ganho de peso interdialítico. Não houve correlação significativa com albumina, ângulo de fase e índice de massa magra. Conclusão: A prevalência de acidose metabólica foi elevada na população em estudo, e um BIC mais baixo correlacionou-se com valores maiores de ureia, PTH, fósforo, ganho de peso interdialítico e menores de IMC. A avaliação do estado metabólico em hemodiálise deve ser implementada rotineiramente, considerando-se os efeitos negativos da acidose no estado nutricional, inflamatório e na doença óssea.

  19. Ginsenoside Rg1 nanoparticle penetrating the blood–brain barrier to improve the cerebral function of diabetic rats complicated with cerebral infarction

    Directory of Open Access Journals (Sweden)

    Shen J

    2017-09-01

    Full Text Available Junyi Shen, Zhiming Zhao, Wei Shang, Chunli Liu, Beibei Zhang, Lingjie Zhao, Hui Cai Department of Integrated Traditional and Western Medicine, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China Abstract: Diabetic cerebral infarction is with poorer prognosis and high rates of mortality. Ginsenoside Rg1 (Rg1 has a wide variety of therapeutic values for central nervous system (CNS diseases for the neuron protective effects. However, the blood–brain barrier (BBB restricts Rg1 in reaching the CNS. In this study, we investigated the therapeutic effects of Rg1 nanoparticle (PHRO, fabricated with γ-PGA, L-PAE (H, Rg1, and OX26 antibody, targeting transferrin receptor, on the diabetes rats complicated with diabetic cerebral infarction in vitro and in vivo. Dynamic light scattering analysis shows the average particle size of PHRO was 79±18 nm and the polydispersity index =0.18. The transmission electron microscope images showed that all NPs were spherical in shape with diameters of 89±23 nm. PHRO released Rg1 with sustained release manner and could promote the migration of cerebrovascular endothelial cells and tube formation and even penetrated the BBB in vitro. PHRO could penetrate the BBB with high concentration in brain tissue to reduce the cerebral infarction volume and promote neuronal recovery in vivo. PHRO was promising to be a clinical treatment of diabetes mellitus with cerebral infarction. Keywords: poly-γ-glutamic acid, ginsenoside Rg1, OX26, blood–brain barrier

  20. Ginsenoside Rg5 improves cognitive dysfunction and beta-amyloid deposition in STZ-induced memory impaired rats via attenuating neuroinflammatory responses.

    Science.gov (United States)

    Chu, Shenghui; Gu, Junfei; Feng, Liang; Liu, Jiping; Zhang, Minghua; Jia, Xiaobin; Liu, Min; Yao, Danian

    2014-04-01

    Neuroinflammatory responses play a crucial role in the pathogenesis of Alzheimer's disease (AD). Ginsenoside Rg5 (Rg5), an abundant natural compound in Panax ginseng, has been found to be beneficial in treating AD. In the present study, we demonstrated that Rg5 improved cognitive dysfunction and attenuated neuroinflammatory responses in streptozotocin (STZ)-induced memory impaired rats. Cognitive deficits were ameliorated with Rg5 (5, 10 and 20mg/kg) treatment in a dose-dependent manner together with decreased levels of inflammatory cytokines TNF-α and IL-1β (Pred and immunohistochemistry staining results showed that Rg5 alleviated Aβ deposition but enhanced the expressions of insulin-like growth factors 1 (IGF-1) and brain derived neurophic factor (BDNF) in the hippocampus and cerebral cortex (Pmemory impairments in rats could be improved by Rg5, which was associated with attenuating neuroinflammatory responses. Our findings suggested that Rg5 could be a beneficial agent for the treatment of AD. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. Ginsenoside Rg1 and platelet-rich fibrin enhance human breast adipose-derived stem cells function for soft tissue regeneration

    Science.gov (United States)

    Li, Hong-Mian; Peng, Qi-Liu; Huang, Min-Hong; Li, De-Quan; Liang, Yi-Dan; Chi, Gang-Yi; Li, De-Hui; Yu, Bing-Chao; Huang, Ji-Rong

    2016-01-01

    Adipose-derived stem cells (ASCs) can be used to repair soft tissue defects, wounds, burns, and scars and to regenerate various damaged tissues. The cell differentiation capacity of ASCs is crucial for engineered adipose tissue regeneration in reconstructive and plastic surgery. We previously reported that ginsenoside Rg1 (G-Rg1 or Rg1) promotes proliferation and differentiation of ASCs in vitro and in vivio. Here we show that both G-Rg1 and platelet-rich fibrin (PRF) improve the proliferation, differentiation, and soft tissue regeneration capacity of human breast adipose-derived stem cells (HBASCs) on collagen type I sponge scaffolds in vitro and in vivo. Three months after transplantation, tissue wet weight, adipocyte number, intracellular lipid, microvessel density, and gene and protein expression of VEGF, HIF-1α, and PPARγ were higher in both G-Rg1- and PRF-treated HBASCs than in control grafts. More extensive new adipose tissue formation was evident after treatment with G-Rg1 or PRF. In summary, G-Rg1 and/or PRF co-administration improves the function of HBASCs for soft tissue regeneration engineering. PMID:27191987

  2. Ginsenoside Rg3 induces DNA damage in human osteosarcoma cells and reduces MNNG-induced DNA damage and apoptosis in normal human cells.

    Science.gov (United States)

    Zhang, Yue-Hui; Li, Hai-Dong; Li, Bo; Jiang, Sheng-Dan; Jiang, Lei-Sheng

    2014-02-01

    Panax ginseng is a Chinese medicinal herb. Ginsenosides are the main bioactive components of P. ginseng, and ginsenoside Rg3 is the primary ginsenoside. Ginsenosides can potently kill various types of cancer cells. The present study was designed to evaluate the potential genotoxicity of ginsenoside Rg3 in human osteosarcoma cells and the protective effect of ginsenoside Rg3 with respect to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage and apoptosis in a normal human cell line (human fibroblasts). Four human osteosarcoma cell lines (MG-63, OS732, U-2OS and HOS cells) and a normal human cell line (human fibroblasts) were employed to investigate the cytotoxicity of ginsenosides Rg3 by MTT assay. Alkaline comet assay and γH2AX focus staining were used to detect the DNA damage in MG-63 and U-2OS cells. The extent of cell apoptosis was determined by flow cytometry and a DNA ladder assay. Our results demonstrated that the cytotoxicity of ginsenoside Rg3 was dose-dependent in the human osteosarcoma cell lines, and MG-63 and U-2OS cells were the most sensitive to ginsenoside Rg3. As expected, compared to the negative control, ginsenoside Rg3 significantly increased DNA damage in a concentration-dependent manner. In agreement with the comet assay data, the percentage of γH2AX-positive MG-63 and U-2OS cells indicated that ginsenoside Rg3 induced DNA double-strand breaks in a concentration-dependent manner. The results also suggest that ginsenoside Rg3 reduces the extent of MNNG-induced DNA damage and apoptosis in human fibroblasts.

  3. Efeitos do treinamento resistido sobre o perfil lipídico de indivíduos com síndrome metabólica

    OpenAIRE

    Albarello, R.A.; Boufleur Farinha, J.; Reckelberg Azambuja, C.; Lopes dos Santos, D.

    2016-01-01

    Objetivo: Investigar os efeitos do treinamento resistido sobre o perfil lipídico de indivíduos com síndrome metabólica. Método: Dez indivíduos sedentários (52.88 ± 10.9 anos) com síndrome metabólica completaram um protocolo de treinamento resistido, com duração total de 15 semanas e frequência semanal de 3 vezes. Antes e após o treinamento resistido, foram analisados parâmetros antropométricos, como massa corporal, índice de massa corporal, circunferências do abdômen, cintura, quadril, per...

  4. Exceso de peso y síndrome metabólico en adolescentes de la Ciudad de México

    OpenAIRE

    Cardoso-Saldaña, Guillermo C.; Yamamoto-Kimura, Liria; Medina-Urrutia, Aída; Posadas-Sánchez, Rosalinda; Caracas-Portilla, Nacú A.; Posadas-Romero, Carlos

    2010-01-01

    Objetivo: Conocer la prevalencia del síndrome metabólico y sus componentes en una muestra de adolescentes de la Ciudad de México. Diseño: Se efectuó un estudio transversal en 772 varones y 1,078 mujeres de 12 a 16 años edad, en ocho escuelas secundarias de la Ciudad de México seleccionadas al azar. Métodos: Se realizaron medidas antropométricas, lípidos y lipoproteínas, Apo-AI y B, glucosa e insulina. Resultados: La prevalencia del síndrome metabólico fue de 12.5%, 11.5% en los varones y 13.5...

  5. Factores de riesgo de síndrome metabólico en estudiantes de la universidad Pablo Guardado Chávez, año 2013

    OpenAIRE

    Bojorges Velázquez, Luis Alberto; Castillo Herrera, José Arcides; Jiménez Tamayo, Roberto

    2013-01-01

    Introducción: en México el síndrome metabólico afecta a más del 25 % de la población, lo cual se atribuye a la combinación de factores genéticos y ambientales. Objetivo: conocer la prevalencia de factores de riesgo de síndrome metabólico en una muestra de estudiantes de la Universidad Pablo Guardado Chávez de la ciudad de Tuxtla Gutiérrez, Chiapas, México. Métodos: se realizó un estudio descriptivo transversal a 190 estudiantes de 17 a 28 años de edad, 99 del sexo femenino y 91 del masculino....

  6. Alterações metabólicas de plantas de milho submetidas à aplicação de glyphosate e fosfito

    OpenAIRE

    Gomes, Giovanna Larissa Gimenes Cotrick [UNESP

    2011-01-01

    O glyphosate é um herbicida de largo espectro de controle, não seletivo, e seu sítio de ação é a inibição da enzima 5-enolpiruvilchiquimato 3-fosfato sintase (EPSPs). Com a inibição da enzima, e o bloqueio da rota do ácido chiquímico pelo glyphosate, ocorre o acúmulo de alguns compostos como os ácidos chiquímico e quínico, além de outras alterações metabólicas e fisiológicas nas plantas. O objetivo deste trabalho foi avaliar as alterações metabólicas e fisiológicas de plantas de milho submeti...

  7. Actividad física, obesidad y factores de riesgo metabólico en niños y adolescentes españoles.

    OpenAIRE

    Laguna Nieto, María

    2013-01-01

    Profundizar en la relación entre la actividad física, la obesidad y el riesgo metabólico en niños y adolescentes resulta determinante bajo una perspectiva clínica. De esta forma, se podrá actuar de manera más eficaz en el tratamiento y prevención de dichas alteraciones. El objetivo general de la presente Tesis Doctoral es estudiar la relación entre actividad física, obesidad y factores de riesgo metabólico en niños y adolescentes. Un total de 439 niños de 9 y 236 adolescentes de 15 años...

  8. Ginsenoside-Rg{sub 1} induces angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a

    Energy Technology Data Exchange (ETDEWEB)

    Kwok, Hoi-Hin [Dr. Gilbert Hung Ginseng Laboratory, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China); Chan, Lai-Sheung [Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China); Poon, Po-Ying [Dr. Gilbert Hung Ginseng Laboratory, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China); Yue, Patrick Ying-Kit [Dr. Gilbert Hung Ginseng Laboratory, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China); Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China); Wong, Ricky Ngok-Shun, E-mail: rnswong@hkbu.edu.hk [Dr. Gilbert Hung Ginseng Laboratory, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China); Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR (China)

    2015-09-15

    Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. Ginsenoside-Rg{sub 1} (Rg{sub 1}), one of the most abundant active components of ginseng, has been demonstrated as an angiogenesis-stimulating compound in different models. There is increasing evidence implicating microRNAs (miRNAs), a group of non-coding RNAs, as important regulators of angiogenesis, but the role of microRNAs in Rg{sub 1}-induced angiogenesis has not been fully explored. In this report, we found that stimulating endothelial cells with Rg{sub 1} could reduce miR-23a expression. In silico experiments predicted hepatocyte growth factor receptor (MET), a well-established mediator of angiogenesis, as the target of miR-23a. Transfection of the miR-23a precursor or inhibitor oligonucleotides validated the inverse relationship of miR-23a and MET expression. Luciferase reporter assays further confirmed the interaction between miR-23a and the MET mRNA 3′-UTR. Intriguingly, ginsenoside-Rg{sub 1} was found to increase MET protein expression in a time-dependent manner. We further demonstrated that ginsenoside-Rg{sub 1}-induced angiogenic activities were indeed mediated through the down-regulation of miR-23a and subsequent up-regulation of MET protein expression, as confirmed by gain- and loss-of-function angiogenic experiments. In summary, our results demonstrated that ginsenoside-Rg{sub 1} could induce angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a. This study has broadened our understanding of the non-genomic effects of ginsenoside-Rg{sub 1,} and provided molecular evidence that warrant further development of natural compound as novel angiogenesis-promoting therapy. - Highlights: • Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. • Ginsenoside-Rg{sub 1} (Rg{sub 1}) has been demonstrated as an angiogenesis-stimulating compound. • We found that Rg{sub 1} induces angiogenesis by

  9. Síndrome metabólica: identificando fatores de risco Metabolic syndrome: identifying the risk factors

    Directory of Open Access Journals (Sweden)

    Simão Augusto Lottenberg

    2007-11-01

    Full Text Available OBJETIVOS Discutir a síndrome metabólica e a identificação de seus fatores de risco, inclusive na faixa etária pediátrica. FONTES DE DADOS: Artigos de revisão indexados. SÍNTESE DOS DADOS:A síndrome metabólica caracteriza-se pela resistência à insulina e pela presença de fatores de risco para doenças cardiovasculares e diabetes melito tipo 2. Não existe ainda um consenso com relação a seus critérios diagnósticos. Na presente revisão, os critérios diagnósticos da Associação Americana de Cardiologia (US National Cholesterol Education Program, Associação Americana de Endocrinologistas Clínicos, Organização Mundial da Saúde e Federação Internacional de Diabetes são apresentados, e as possibilidades de aplicação dos mesmos na infância são discutidas. São também abordados os aspectos fisiopatológicos da síndrome, principalmente aqueles relacionados ao período perinatal e à infância. CONCLUSÃO: A síndrome metabólica tem sido identificada de forma cada vez mais freqüente, principalmente durante a adolescência. Mudanças de estilo de vida, como alimentação e atividade física, são fundamentais na sua prevenção e tratamento. Tratamento medicamentoso e, eventualmente, tratamento cirúrgico também devem ser considerados, dependendo da gravidade, mesmo nesta fase da vida.OBJECTIVES: To discuss the metabolic syndrome and identify its risk factors, including in the pediatric age group. SOURCES: Indexed review articles. SUMMARY OF THE FINDINGS: The metabolic syndrome is characterized by insulin resistance and the presence of risk factors for cardiovascular diseases and diabetes mellitus type 2. Consensus has not yet been reached on its diagnostic criteria. This review presents diagnostic criteria defined by the American Heart Association (US National Cholesterol Education Program, the American Association of Clinical Endocrinologists, the World Health Organization and the International Diabetes Federation

  10. Excesso de peso e gordura abdominal para a síndrome metabólica em nipo-brasileiros

    Directory of Open Access Journals (Sweden)

    Lerario Daniel D G

    2002-01-01

    Full Text Available OBJETIVO: A obesidade, especialmente de distribuição abdominal, associa-se a fatores de risco cardiovasculares como a dislipidemia, a hipertensão arterial (HA e o diabetes mellitus (DM. A importância desses fatores em nipo-brasileiros foi previamente demonstrada, apesar de a obesidade não ser característica marcante dos migrantes japoneses. Realizou-se estudo com o objetivo de avaliar a prevalência de excesso de peso e a adiposidade central (AC em nipo-brasileiros e suas relações com distúrbios metabólicos. MÉTODOS: A amostra incluiu 530 nipo-brasileiros (40-79 anos de primeira e segunda gerações, submetidos a medidas antropométricas de pressão arterial, perfil lipídico e teste oral de tolerância à glicose. A prevalência (por ponto e intervalo de confiança de excesso de peso foi calculada pelo valor de corte >26,4 kg/m². O diagnóstico de AC foi baseado na razão entre as circunferências da cintura e do quadril (RCQ, sendo que valores > ou = 0,85 e > ou = 0,95, para mulheres e homens, respectivamente, firmavam esse diagnóstico. RESULTADOS: A prevalência de excesso de peso foi de 22,4% (IC95%-- 20,6-28,1, e a de AC, de 67,0% (IC95% -- 63,1-70,9. Além de maiores prevalências de DM, HA e dislipidemia, estratificando-se pelo índice de massa corporal (IMC e RCQ, indivíduos com excesso de peso e adiposidade central apresentaram pior perfil metabólico: a pressão arterial foi significantemente maior naqueles com excesso de peso, sem e com AC; indivíduos com AC apresentaram maiores índices de glicemia, triglicerídeos, colesterol total e LDL e menor HDL quando comparados aos sem excesso de peso e sem AC; a insulinemia de jejum foi significantemente maior em indivíduos com excesso de peso (sem e com AC do que naqueles sem excesso de peso e sem AC. CONCLUSÃO: A comparação de subgrupos com e sem adiposidade central foi compatível com a hipótese de que a deposição abdominal de gordura representa fator de risco para

  11. Excesso de peso e gordura abdominal para a síndrome metabólica em nipo-brasileiros

    Directory of Open Access Journals (Sweden)

    Daniel D G Lerario

    2002-02-01

    Full Text Available OBJETIVO: A obesidade, especialmente de distribuição abdominal, associa-se a fatores de risco cardiovasculares como a dislipidemia, a hipertensão arterial (HA e o diabetes mellitus (DM. A importância desses fatores em nipo-brasileiros foi previamente demonstrada, apesar de a obesidade não ser característica marcante dos migrantes japoneses. Realizou-se estudo com o objetivo de avaliar a prevalência de excesso de peso e a adiposidade central (AC em nipo-brasileiros e suas relações com distúrbios metabólicos. MÉTODOS: A amostra incluiu 530 nipo-brasileiros (40-79 anos de primeira e segunda gerações, submetidos a medidas antropométricas de pressão arterial, perfil lipídico e teste oral de tolerância à glicose. A prevalência (por ponto e intervalo de confiança de excesso de peso foi calculada pelo valor de corte >26,4 kg/m². O diagnóstico de AC foi baseado na razão entre as circunferências da cintura e do quadril (RCQ, sendo que valores > ou = 0,85 e > ou = 0,95, para mulheres e homens, respectivamente, firmavam esse diagnóstico. RESULTADOS: A prevalência de excesso de peso foi de 22,4% (IC95%-- 20,6-28,1, e a de AC, de 67,0% (IC95% -- 63,1-70,9. Além de maiores prevalências de DM, HA e dislipidemia, estratificando-se pelo índice de massa corporal (IMC e RCQ, indivíduos com excesso de peso e adiposidade central apresentaram pior perfil metabólico: a pressão arterial foi significantemente maior naqueles com excesso de peso, sem e com AC; indivíduos com AC apresentaram maiores índices de glicemia, triglicerídeos, colesterol total e LDL e menor HDL quando comparados aos sem excesso de peso e sem AC; a insulinemia de jejum foi significantemente maior em indivíduos com excesso de peso (sem e com AC do que naqueles sem excesso de peso e sem AC. CONCLUSÃO: A comparação de subgrupos com e sem adiposidade central foi compatível com a hipótese de que a deposição abdominal de gordura representa fator de risco para

  12. Impacto de las nuevas definiciones en la prevalencia del síndrome metabólico en una población adulta de Bucaramanga, Colombia

    Directory of Open Access Journals (Sweden)

    Juan Bernardo Pinzón

    2007-06-01

    Conclusión. Las nuevas definiciones para el síndrome metabólico (ATP-IIIa e IDF aumentan tres veces la prevalencia del diagnóstico, sin encontrar diferencias entre ellas, a pesar de que la última organización incluye la obesidad central como un criterio necesario para el diagnóstico, cuando en la primera no se hace igual ponderación.

  13. Origem da síndrome metabólica: aspectos genético-evolutivos e nutricionais = Origin of the metabolic syndrome: genetic, evolutionary and nutritional aspects

    Directory of Open Access Journals (Sweden)

    Gottlieb, Maria Gabriela Valle

    2008-01-01

    Conclusões: a programação genética fetal pode ser afetada pela nutrição materna, o que pode levar a distúrbios no metabolismo energético. Além disso, uma vida sedentária, aliada a alta ingestão calórica, pode desencadear a Síndrome Metabólica

  14. Índice de adiposidad corporal como predictor de obesidad y de síndrome metabólico en adultos de Bogotá, Colombia

    OpenAIRE

    González-Ruíz, Katherine; Ramírez-Vélez, Robinson; Correa Bautista, Jorge Enrique

    2015-01-01

    Recientemente, Bergman et al. desarrollaron el índice de adiposidad corporal (IAC), como un marcador de obesidad por exceso de grasa corporal en la práctica clínica. En este estudio se evaluó la validez del IAC como marcador de obesidad por exceso de adiposidad, además de examinar la capacidad predictiva del IAC con componentes e índices metabólicos asociados al SM en adultos de Bogotá, Colombia.

  15. The Poet of Borcali Zərgər Musa and his Poems

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    Bahadır GÜNEŞ

    2017-12-01

    Full Text Available Today it is divided into different regions and borrowed under the administration of the Republic of Georgia, living as an old Turkish homeland from old dates. Due to being located in the connection area between Turkistan and Anatolia, together with the Turks, it is one of the war zones of different ethnic communities. This situation manifested itself as the fusion of diversity in the cultural sense, and it has opened the way for the way of showing tricks live to this day. There are a lot of poets in love today in Borcali. These poets both in with saz and solo they live their art in different regions of the Turkish world. Among the poets waiting to be discovered by researchers there are also those who live the last days of their life. Therefore, especially Turkish and Turkish language and literature researchers from different disciplines must be in this geography. In this study, born and raised in Borcali a unknown poet of many and the region known by the name Zərgər Musa,Borcali poet introduced and the poet’s book by the name Bir Çinarın Dərdi will be evaluated in terms of content, expression forms and pattern expressions.

  16. The histone deacetylase inhibiting drug Entinostat induces lipid accumulation in differentiated HepaRG cells

    Science.gov (United States)

    Nunn, Abigail D. G.; Scopigno, Tullio; Pediconi, Natalia; Levrero, Massimo; Hagman, Henning; Kiskis, Juris; Enejder, Annika

    2016-06-01

    Dietary overload of toxic, free metabolic intermediates leads to disrupted insulin signalling and fatty liver disease. However, it was recently reported that this pathway might not be universal: depletion of histone deacetylase (HDAC) enhances insulin sensitivity alongside hepatic lipid accumulation in mice, but the mechanistic role of microscopic lipid structure in this effect remains unclear. Here we study the effect of Entinostat, a synthetic HDAC inhibitor undergoing clinical trials, on hepatic lipid metabolism in the paradigmatic HepaRG liver cell line. Specifically, we statistically quantify lipid droplet morphology at single cell level utilizing label-free microscopy, coherent anti-Stokes Raman scattering, supported by gene expression. We observe Entinostat efficiently rerouting carbohydrates and free-fatty acids into lipid droplets, upregulating lipid coat protein gene Plin4, and relocating droplets nearer to the nucleus. Our results demonstrate the power of Entinostat to promote lipid synthesis and storage, allowing reduced systemic sugar levels and sequestration of toxic metabolites within protected protein-coated droplets, suggesting a potential therapeutic strategy for diseases such as diabetes and metabolic syndrome.

  17. Rigorous RG Algorithms and Area Laws for Low Energy Eigenstates in 1D

    Science.gov (United States)

    Arad, Itai; Landau, Zeph; Vazirani, Umesh; Vidick, Thomas

    2017-11-01

    One of the central challenges in the study of quantum many-body systems is the complexity of simulating them on a classical computer. A recent advance (Landau et al. in Nat Phys, 2015) gave a polynomial time algorithm to compute a succinct classical description for unique ground states of gapped 1D quantum systems. Despite this progress many questions remained unsolved, including whether there exist efficient algorithms when the ground space is degenerate (and of polynomial dimension in the system size), or for the polynomially many lowest energy states, or even whether such states admit succinct classical descriptions or area laws. In this paper we give a new algorithm, based on a rigorously justified RG type transformation, for finding low energy states for 1D Hamiltonians acting on a chain of n particles. In the process we resolve some of the aforementioned open questions, including giving a polynomial time algorithm for poly( n) degenerate ground spaces and an n O(log n) algorithm for the poly( n) lowest energy states (under a mild density condition). For these classes of systems the existence of a succinct classical description and area laws were not rigorously proved before this work. The algorithms are natural and efficient, and for the case of finding unique ground states for frustration-free Hamiltonians the running time is {\\tilde{O}(nM(n))} , where M( n) is the time required to multiply two n × n matrices.

  18. Test set up description and performances for HAWAII-2RG detector characterization at ESTEC

    Science.gov (United States)

    Crouzet, P.-E.; ter Haar, J.; de Wit, F.; Beaufort, T.; Butler, B.; Smit, H.; van der Luijt, C.; Martin, D.

    2012-07-01

    In the frame work of the European Space Agency's Cosmic Vision program, the Euclid mission has the objective to map the geometry of the Dark Universe. Galaxies and clusters of galaxies will be observed in the visible and near-infrared wavelengths by an imaging and spectroscopic channel. For the Near Infrared Spectrometer instrument (NISP), the state-of-the-art HAWAII-2RG detectors will be used, associated with the SIDECAR ASIC readout electronic which will perform the image frame acquisitions. To characterize and validate the performance of these detectors, a test bench has been designed, tested and validated. This publication describes the pre-tests performed to build the set up dedicated to dark current measurements and tests requiring reasonably uniform light levels (such as for conversion gain measurements). Successful cryogenic and vacuum tests on commercial LEDs and photodiodes are shown. An optimized feed through in stainless steel with a V-groove to pot the flex cable connecting the SIDECAR ASIC to the room temperature board (JADE2) has been designed and tested. The test set up for quantum efficiency measurements consisting of a lamp, a monochromator, an integrating sphere and set of cold filters, and which is currently under construction will ensure a uniform illumination across the detector with variations lower than 2%. A dedicated spot projector for intra-pixel measurements has been designed and built to reach a spot diameter of 5 μm at 920nm with 2nm of bandwidth [1].

  19. Laboratory Measurement of the Brighter-fatter Effect in an H2RG Infrared Detector

    Science.gov (United States)

    Plazas, A. A.; Shapiro, C.; Smith, R.; Huff, E.; Rhodes, J.

    2018-06-01

    The “brighter-fatter” (BF) effect is a phenomenon—originally discovered in charge coupled devices—in which the size of the detector point-spread function (PSF) increases with brightness. We present, for the first time, laboratory measurements demonstrating the existence of the effect in a Hawaii-2RG HgCdTe near-infrared (NIR) detector. We use JPL’s Precision Projector Laboratory, a facility for emulating astronomical observations with UV/VIS/NIR detectors, to project about 17,000 point sources onto the detector to stimulate the effect. After calibrating the detector for nonlinearity with flat-fields, we find evidence that charge is nonlinearly shifted from bright pixels to neighboring pixels during exposures of point sources, consistent with the existence of a BF-type effect. NASAs Wide Field Infrared Survey Telescope (WFIRST) will use similar detectors to measure weak gravitational lensing from the shapes of hundreds of million of galaxies in the NIR. The WFIRST PSF size must be calibrated to ≈0.1% to avoid biased inferences of dark matter and dark energy parameters; therefore further study and calibration of the BF effect in realistic images will be crucial.

  20. Multidisciplinary Identification of the Controversial Freedom Fighter Jörg Jenatsch, Assassinated 1639 in Chur, Switzerland.

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    Martin Haeusler

    Full Text Available Jörg Jenatsch, a leading freedom fighter during the Thirty Year's War in Graubünden, Switzerland, was assassinated on carnival 1639. Jenatsch's controversial biography and the unclear circumstances of his death inspired the formation of various legends, novels and films. In 1959, a skeleton discovered in the cathedral of Chur with remains of wealthy baroque clothing was tentatively attributed to Jenatsch. Here, we reassess the skeleton based on a new exhumation. Our multidisciplinary analysis and the head injuries are consistent with reports of the eyewitnesses of the crime, demonstrating that Jenatsch was killed from behind with a semi-sharp implement, supposedly an axe, as well as by a blow with a broad-surfaced object. Moreover, our facial reconstruction closely matches an oil portrait of Jenatsch, and the HIrisPlex system applied to DNA-extracts from the femoral bone reveals brown eye and dark brown hair colour, which coincides well with the portrait, too. Finally, isotope analysis of the femoral bone and a molar support Jenatsch's high social status, luxury diet and a high mobility in the last decade of his life. This multidisciplinary approach thus reinforces personal identification and provides additional insight into the life of this important historic person beyond written resources.

  1. Multidisciplinary Identification of the Controversial Freedom Fighter Jörg Jenatsch, Assassinated 1639 in Chur, Switzerland.

    Science.gov (United States)

    Haeusler, Martin; Haas, Cordula; Lösch, Sandra; Moghaddam, Negahnaz; Villa, Igor M; Walsh, Susan; Kayser, Manfred; Seiler, Roger; Ruehli, Frank; Janosa, Manuel; Papageorgopoulou, Christina

    2016-01-01

    rg Jenatsch, a leading freedom fighter during the Thirty Year's War in Graubünden, Switzerland, was assassinated on carnival 1639. Jenatsch's controversial biography and the unclear circumstances of his death inspired the formation of various legends, novels and films. In 1959, a skeleton discovered in the cathedral of Chur with remains of wealthy baroque clothing was tentatively attributed to Jenatsch. Here, we reassess the skeleton based on a new exhumation. Our multidisciplinary analysis and the head injuries are consistent with reports of the eyewitnesses of the crime, demonstrating that Jenatsch was killed from behind with a semi-sharp implement, supposedly an axe, as well as by a blow with a broad-surfaced object. Moreover, our facial reconstruction closely matches an oil portrait of Jenatsch, and the HIrisPlex system applied to DNA-extracts from the femoral bone reveals brown eye and dark brown hair colour, which coincides well with the portrait, too. Finally, isotope analysis of the femoral bone and a molar support Jenatsch's high social status, luxury diet and a high mobility in the last decade of his life. This multidisciplinary approach thus reinforces personal identification and provides additional insight into the life of this important historic person beyond written resources.

  2. Fatores associados à efetividade da doação de órgãos e tecidos para transplantes

    OpenAIRE

    Freire, Izaura Luzia Silverio

    2013-01-01

    O transplante de órgãos e tecidos apresenta-se como importante opção terapêutica, tanto do ponto de vista médico, quanto social ou econômico. Assim, a identificação das variáveis que podem interferir na efetividade da doação de órgãos e tecidos para transplante precisa ser investigada de maneira adequada, pois se está diante de crescente índice de doenças crônicas e degenerativas na população, que faz com que a lista de espera para transplante aumente desproporcionalmente e os pacientes chega...

  3. Processo de transporte de órgãos para transplante na Cidade de São Paulo

    Directory of Open Access Journals (Sweden)

    Adriana Roberta Lira

    2016-06-01

    Full Text Available O trabalho da Polícia Militar do Estado de São Paulo, em favor da sociedade completa 185 anos de existência. É um trabalho amplo e digno. Uma parcela desse trabalho apoia-se no Grupamento Aéreo da Polícia Militar do Estado de São Paulo, guarnição direcionada especificamente à atividade de transportar órgãos para transplante. Nesse sentido, o presente artigo, objetiva avaliar essa atividade, baseando-se em pesquisa realizada junto às Instituições e profissionais responsáveis pela captação e o transporte de órgãos para transplante, e sugere também ações de melhorias no planejamento dessa complexa operação.

  4. Morphine dependence is attenuated by red ginseng extract and ginsenosides Rh2, Rg3, and compound K

    OpenAIRE

    Yayeh, Taddesse; Yun, Kyunghwa; Jang, Soyong; Oh, Seikwan

    2016-01-01

    Background: Red ginseng and ginsenosides have shown plethoric effects against various ailments. However, little is known regarding the effect of red ginseng on morphine-induced dependence and tolerance. We therefore investigated the effect of red ginseng extract (RGE) and biotransformed ginsenosides Rh2, Rg3, and compound K on morphine-induced dependence in mice and rats. Methods: While mice were pretreated with RGE and then morphine was injected intraperitoneally, rats were infused with g...

  5. N-(4-Hydroxyphenyl) retinamide potentiated paclitaxel for cell cycle arrest and apoptosis in glioblastoma C6 and RG2 cells

    OpenAIRE

    Janardhanan, Rajiv; Butler, Jonathan T.; Banik, Naren L.; Ray, Swapan K.

    2009-01-01

    Glioblastoma grows aggressively due to its ability to maintain abnormally high potentials for cell proliferation. The present study examines the synergistic actions of N-(4-hdroxyphenyl) retinamide (4-HPR) and paclitaxel (PTX) to control the growth of rat glioblastoma C6 and RG2 cell lines. 4-HPR induced astrocytic differentiation was accompanied by increased expression of the tight junction protein e-cadherin and sustained down regulation of Id2 (member of inhibitor of differentiation family...

  6. Animal study of rh-endostatin combined with ginsenoside Rg3 on inhibiting growth of breast cancer xenografts

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    Chuan-Ming Tong

    2016-03-01

    Full Text Available Objective: To study the inhibiting effect of rh-endostatin combined with ginsenoside Rg3 on growth of breast cancer xenografts. Methods: C57BL/6 nude mice were selected as research subjects, animal models with breast cancer xenografts were established through subcutaneous injection of breast cancer cells in the neck and randomly divided into A-D groups, and normal saline, rh-endostatin, ginsenoside Rg3 and rh-endostatin combined with ginsenoside Rg3 were injected respectively. Tumor growth, serum angiogenesis factor contents, percentages of cell cycles and expression levels of cell cycle-related molecules in tumor tissue of four groups were compared. Results: 7 d, 14 d and 21 d after treatment, tumor volume as well as serum VEGF and bFGF contents of B group, C group and D group were significantly lower than those of A group, and tumor volume as well as serum VEGF and bFGF contents of D group were significantly lower than those of B group and C group; 21 d after treatment, tumor weight, percentages of S phase and G2/M phase as well as mRNA contents and protein contents of cyclin E and CDC25A in tumor tissue of B group, C group and D group were lower than those of A group, and G0/G1 phase percentages were higher than that of A group; tumor weight, percentages of S phase and G2/M phase as well as mRNA contents and protein contents of cyclin E and CDC25A in tumor tissue of D group were significantly lower than those of B group and C group, and G0/G1 phase percentage was lower than those of B group and C group. Conclusion: rh-endostatin combined with ginsenoside Rg3 can more effectively inhibit growth of breast cancer xenografts, make cell cycle arrest and down-regulate expression of cell cyclerelated molecules.

  7. The Rg1 allele as a valuable tool for genetic transformation of the tomato 'Micro-Tom' model system

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    Quecini Vera

    2010-10-01

    Full Text Available Abstract Background The cultivar Micro-Tom (MT is regarded as a model system for tomato genetics due to its short life cycle and miniature size. However, efforts to improve tomato genetic transformation have led to protocols dependent on the costly hormone zeatin, combined with an excessive number of steps. Results Here we report the development of a MT near-isogenic genotype harboring the allele Rg1 (MT-Rg1, which greatly improves tomato in vitro regeneration. Regeneration was further improved in MT by including a two-day incubation of cotyledonary explants onto medium containing 0.4 μM 1-naphthaleneacetic acid (NAA before cytokinin treatment. Both strategies allowed the use of 5 μM 6-benzylaminopurine (BAP, a cytokinin 100 times less expensive than zeatin. The use of MT-Rg1 and NAA pre-incubation, followed by BAP regeneration, resulted in high transformation frequencies (near 40%, in a shorter protocol with fewer steps, spanning approximately 40 days from Agrobacterium infection to transgenic plant acclimatization. Conclusions The genetic resource and the protocol presented here represent invaluable tools for routine gene expression manipulation and high throughput functional genomics by insertional mutagenesis in tomato.

  8. Relativity-Induced Bonding Pattern Change in Coinage Metal Dimers M2 (M = Cu, Ag, Au, Rg).

    Science.gov (United States)

    Li, Wan-Lu; Lu, Jun-Bo; Wang, Zhen-Ling; Hu, Han-Shi; Li, Jun

    2018-05-07

    The periodic table provides a fundamental protocol for qualitatively classifying and predicting chemical properties based on periodicity. While the periodic law of chemical elements had already been rationalized within the framework of the nonrelativistic description of chemistry with quantum mechanics, this law was later known to be affected significantly by relativity. We here report a systematic theoretical study on the chemical bonding pattern change in the coinage metal dimers (Cu 2 , Ag 2 , Au 2 , Rg 2 ) due to the relativistic effect on the superheavy elements. Unlike the lighter congeners basically demonstrating ns- ns bonding character and a 0 g + ground state, Rg 2 shows unique 6d-6d bonding induced by strong relativity. Because of relativistic spin-orbit (SO) coupling effect in Rg 2 , two nearly degenerate SO states, 0 g + and 2 u , exist as candidate of the ground state. This relativity-induced change of bonding mechanism gives rise to various unique alteration of chemical properties compared with the lighter dimers, including higher intrinsic bond energy, force constant, and nuclear shielding. Our work thus provides a rather simple but clear-cut example, where the chemical bonding picture is significantly changed by relativistic effect, demonstrating the modified periodic law in heavy-element chemistry.

  9. Diretrizes para avaliação e validação do potencial doador de órgãos em morte encefálica

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    Glauco Adrieno Westphal

    Full Text Available RESUMO O transplante de órgãos é a única alternativa para muitos pacientes portadores de algumas doenças terminais. Ao mesmo tempo, é preocupante a crescente desproporção entre a alta demanda por transplantes de órgãos e o baixo índice de transplantes efetivados. Dentre as diferentes causas que alimentam essa desproporção, estão os equívocos na identificação do potencial doador de órgãos e as contraindicações mal atribuídas pela equipe assistente. Assim, o presente documento pretende fornecer subsídios à equipe multiprofissional da terapia intensiva para o reconhecimento, a avaliação e a validação do potencial doador de órgãos.

  10. Ansiedade, depressão e estresse em pacientes com síndrome metabólica

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    Martha Wallig Brusius Ludwig

    2012-01-01

    Full Text Available El Síndrome Metabólico (SM es un conjunto de factores de riesgo para enfermedades cardiovasculares relacionadas con un estilo de vida poco saludable. Algunos estudios indican la presencia de complicaciones psicológicas como la depresión, la ansiedad y el estrés en estos pacientes. La evaluación de las mismas es fundamental para un tratamiento individualizado visando la mejora y la adhesión del paciente a las directrices. El estudio pretende presentar la descripción socio demográfica de ansiedad, depresión y de estrés de los participantes del programa de Modificación de estilo de vida y el riesgo cardiovascular, desarrollado en la ciudad de Porto Alegre, RS. De los 38 participantes, la mayoría era del género femenino (78,9%, con educación superior completa (42,1%. En cuanto a otros aspectos evaluados, el 31,6% tenían síntomas moderados de depresión, el 55,3% se encontraban en la etapa de la resistencia de estrés, el 47,4% tenían síntomas mínimos de ansiedad y el 86,8% presenta alta probabilidad de desarrollo de enfermedades relacionadas con el estrés.

  11. Dinámicas del capitalismo : escisión metabólica y sacrificio del valor de uso

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    Julio Peña y Lillo E.

    2013-10-01

    Full Text Available El presente artículo pretende indagar los elementos que hacen parte de la dinámica de funcionamiento del sistema capitalista. Estos elementos vinculados con el sacrificio del valor de uso así como con la escisión metabólica, son piezas fundamentales en la conformación de la crisis estructural del modo de producción y reproducción capitalista, los cuales se conjugan y articulan en el proceso de perpetuación de las crisis ambientales. A través de las categorías de análisis que nos brinda el marxismo, en su vertiente ecológica, nos planteamos la incorporación del concepto de biocentrismo, como una respuesta o alternativa a la ética antropocéntrica (del individualismo productivista cuya relevancia prima en el reconocimiento y respeto de la naturaleza y todas sus entidades vivas.

  12. 20(S-ginsenoside Rg3 promotes senescence and apoptosis in gallbladder cancer cells via the p53 pathway

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    Zhang F

    2015-08-01

    Full Text Available Fei Zhang,* Maolan Li,* Xiangsong Wu,* Yunping Hu, Yang Cao, Xu’an Wang, Shanshan Xiang, Huaifeng Li, Lin Jiang, Zhujun Tan, Wei Lu, Hao Weng, Yijun Shu, Wei Gong, Xuefeng Wang, Yong Zhang, Weibin Shi, Ping Dong,# Jun Gu,# Yingbin Liu#Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work #These authors jointly directed this work Abstract: Gallbladder cancer (GBC, the most frequent malignancy of the biliary tract, is associated with high mortality and extremely poor prognosis. 20(S-ginsenoside Rg3 (20(S-Rg3 is a steroidal saponin with high pharmacological activity. However, the anticancer effect of 20(S-Rg3 in human GBC has not yet been determined. In this study, we primarily found that 20(S-Rg3 exposure suppressed the survival of both NOZ and GBC-SD cell lines in a concentration-dependent manner. Moreover, induction of cellular senescence and G0/G1 arrest by 20(S-Rg3 were accompanied by a large accumulation of p53 and p21 as a result of murine double minute 2 (MDM2 inhibition. 20(S-Rg3 also caused a remarkable increase in apoptosis via the activation of the mitochondrial-mediated intrinsic caspase pathway. Furthermore, intraperitoneal injection of 20(S-Rg3 (20 or 40 mg/kg for 3 weeks markedly inhibited the growth of xenografts in nude mice. Our results demonstrated that 20(S-Rg3 potently inhibited growth and survival of GBC cells both in vitro and in vivo. 20(S-Rg3 attenuated GBC growth probably via activation of the p53 pathway, and subsequent induction of cellular senescence and mitochondrial-dependent apoptosis. Therefore, 20(S-Rg3 may be a potential chemotherapeutic agent for GBC therapy.Keywords: gallbladder cancer, 20(S-ginsenoside Rg3, senescence, apoptosis, p53 pathway

  13. Pharmacokinetics and efficiency of brain targeting of ginsenosides Rg1 and Rb1 given as Nao-Qing microemulsion.

    Science.gov (United States)

    Li, Tao; Shu, Ya-Jun; Cheng, Jia-Yin; Liang, Run-Cheng; Dian, Shao-Na; Lv, Xiao-Xun; Yang, Meng-Qi; Huang, Shu-Ling; Chen, Gang; Yang, Fan

    2015-02-01

    Nao-Qing solution has been shown to be clinically effective in the treatment of acute ischemic stroke (AIS). The purpose of this study was to improve the pharmacokinetics and brain uptake of Nao-Qing, administered as an oil-in-water microemulsion. Sprague-Dawley (SD) rats were given Nao-Qing microemulsion by intranasal or intragastric routes. Samples of blood, brain, heart, liver, lung and kidney were collected at pre-determined time intervals, and the contents of ginsenosides Rg1 and Rb1 (active ingredients of the Nao-Qing microemulsion) were analyzed by high-performance liquid chromatography (HPLC). The results showed that contents of ginsenosides Rg1 and Rb1 in Nao-Qing microemulsion was 8475.13 ± 54.61 μg/ml and 6633.42 ± 527.27 μg/ml, respectively, and that the particle size, pH and viscosity of the microemulsion were 19.9 ± 5.07 nm, 6.1 and 3.056 × 10(-3 )Pas, respectively. Absorption of ginsenoside Rg1 was higher than that of ginsenoside Rb1, which was barely detectable after intragastric administration; furthermore, the concentration of ginsenoside Rg1 in blood and other tissues at each time point was lower for intragastric than for intranasal administration. Compared with intragastric administration, intranasal administration resulted in a shorter tmax (0.08 versus 1 h), a higher Cmax (16.65 versus 11.29 μg/ml), and a higher area under the concentration-time curve (AUC) (592.91 versus 101.70 μgċh/ml) in the brain. The relative rates of uptake (Re) and the ratio of peak concentration (Ce) in the brain were 126.31% and 147.48% for ginsenoside Rg1, respectively. These data illustrate that intranasal administration can promote the absorption of drugs in Nao-Qing microemulsion and achieve fast effect.

  14. The 3D Recognition, Generation, Fusion, Update and Refinement (RG4) Concept

    Science.gov (United States)

    Maluf, David A.; Cheeseman, Peter; Smelyanskyi, Vadim N.; Kuehnel, Frank; Morris, Robin D.; Norvig, Peter (Technical Monitor)

    2001-01-01

    This paper describes an active (real time) recognition strategy whereby information is inferred iteratively across several viewpoints in descent imagery. We will show how we use inverse theory within the context of parametric model generation, namely height and spectral reflection functions, to generate model assertions. Using this strategy in an active context implies that, from every viewpoint, the proposed system must refine its hypotheses taking into account the image and the effect of uncertainties as well. The proposed system employs probabilistic solutions to the problem of iteratively merging information (images) from several viewpoints. This involves feeding the posterior distribution from all previous images as a prior for the next view. Novel approaches will be developed to accelerate the inversion search using novel statistic implementations and reducing the model complexity using foveated vision. Foveated vision refers to imagery where the resolution varies across the image. In this paper, we allow the model to be foveated where the highest resolution region is called the foveation region. Typically, the images will have dynamic control of the location of the foveation region. For descent imagery in the Entry, Descent, and Landing (EDL) process, it is possible to have more than one foveation region. This research initiative is directed towards descent imagery in connection with NASA's EDL applications. Three-Dimensional Model Recognition, Generation, Fusion, Update, and Refinement (RGFUR or RG4) for height and the spectral reflection characteristics are in focus for various reasons, one of which is the prospect that their interpretation will provide for real time active vision for automated EDL.

  15. Differential toxicity of heterocyclic aromatic amines and their mixture in metabolically competent HepaRG cells

    International Nuclear Information System (INIS)

    Dumont, Julie; Josse, Rozenn; Lambert, Carine; Antherieu, Sebastien; Le Hegarat, Ludovic; Aninat, Caroline; Robin, Marie-Anne; Guguen-Guillouzo, Christiane

    2010-01-01

    Human exposure to heterocyclic aromatic amines (HAA) usually occurs through mixtures rather than individual compounds. However, the toxic effects and related mechanisms of co-exposure to HAA in humans remain unknown. We compared the effects of two of the most common HAA, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), individually or in combination, in the metabolically competent human hepatoma HepaRG cells. Various endpoints were measured including cytotoxicity, apoptosis, oxidative stress and DNA damage by the comet assay. Moreover, the effects of PhIP and/or MeIQx on mRNA expression and activities of enzymes involved in their activation and detoxification pathways were evaluated. After a 24 h treatment, PhIP and MeIQx, individually and in combination, exerted differential effects on apoptosis, oxidative stress, DNA damage and cytochrome P450 (CYP) activities. Only PhIP induced DNA damage. It was also a stronger inducer of CYP1A1 and CYP1B1 expression and activity than MeIQx. In contrast, only MeIQx exposure resulted in a significant induction of CYP1A2 activity. The combination of PhIP with MeIQx induced an oxidative stress and showed synergistic effects on apoptosis. However, PhIP-induced genotoxicity was abolished by a co-exposure with MeIQx. Such an inhibitory effect could be explained by a significant decrease in CYP1A2 activity which is responsible for PhIP genotoxicity. Our findings highlight the need to investigate interactions between HAA when assessing risks for human health and provide new insights in the mechanisms of interaction between PhIP and MeIQx.

  16. RG flow and thermodynamics of causal horizons in higher-derivative AdS gravity

    International Nuclear Information System (INIS)

    Banerjee, Shamik; Bhattacharyya, Arpan

    2016-01-01

    In http://arxiv.org/abs/1508.01343 [hep-th], one of the authors proposed that in AdS/CFT the gravity dual of the boundary c-theorem is the second law of thermodynamics satisfied by causal horizons in AdS and this was verified for Einstein gravity in the bulk. In this paper we verify this for higher derivative theories. We pick up theories for which an entropy expression satisfying the second law exists and show that the entropy density evaluated on the causal horizon in a RG flow geometry is a holographic c-function. We also prove that given a theory of gravity described by a local covariant action in the bulk a sufficient condition to ensure holographic c-theorem is that the second law of causal horizon thermodynamics be satisfied by the theory. This allows us to explicitly construct holographic c-function in a theory where there is curvature coupling between gravity and matter and standard null energy condition cannot be defined although second law is known to hold. Based on the duality between c-theorem and the second law of causal horizon thermodynamics proposed in http://arxiv.org/abs/1508.01343 [hep-th] and the supporting calculations of this paper we conjecture that every Unitary higher derivative theory of gravity in AdS satisfies the second law of causal horizon thermodynamics. If this is not true then c-theorem will be violated in a unitary Lorentz invariant field theory.

  17. Estudos sôbre os órgãos odoríferos de alguns Hesperidae Brasileiros

    Directory of Open Access Journals (Sweden)

    Rudolf Barth

    1952-03-01

    Full Text Available 1. São descritas áreas de escamas nas asas do macho e da fêmea de várias espécies de Hesperidae brasilienses. 2. É considerada a corelação destas áreas as articulações entre as asas anteriores e posteriores. 3. Verificou-se que as áreas das escamas odoríferas da asa posterior têm origem nas áreas das escamas de ligação. Esta mudança de função é demonstrada pela forma das escamas em comparação com escamas de ligação em função. 4. É apresentada uma descrição detalhada da estrutura mais fina das escamas de ligação e das escamas odoríferas. 5. As borboletas de sexo masculino das espécies examinadas podem ser divididas de acôrdo com a ausência ou presença de órgãos odoríferos diferentes (v. tabela 1: 1. grupo: borboletas sem glândulas odoríferas especiais (tipo de Vehilius. 2. grupo: borboletas com sulco odorífero na asa anterior (tipo de Augiades 3. grupo: borboletas com dobra costal da asa anterior (tipo de Thanaos. 4. grupo: borboletas com órgão odorífero no metatórax (tipo de Achlyodes. 6. A fêmea de H 31 possui na memebrana intersegmental VII./VIII. escamas odoríferas de uma notável estrutura mais fina. 7. As escamas odoríferas são estudadas com respeito à sua estrutura fina e dada uma possível derivação da cerda da escama. 8. O estudo dos órgãos odoríferos é feito com respeito ao aumento da superfície destinada á evaporação pelas escamas e ainda á função protetora das escamas de cobertura e dobras cuticulares. 9. A evolução de órgãos odoríferos diferentes em diferentes partes do corpo indica uma evolução polifilética dos órgãos, o que tem valor sistemático na família bem limitada das Hesperidae. 10. Os machos de h 16 e H 130 possuem dois tipos de órgãos odoríferos especiais, a dobra costal e o órgão odorífero do metatórax com pincel-distribuidor na tíbia posterior. Nas espécies citadas e que têm parentesco, toma lugar uma evolução polifilética de órg

  18. Effects of Ginsenoside Rg1 on Learning and Memory in a Reward-directed Instrumental Conditioning Task in Chronic Restraint Stressed Rats.

    Science.gov (United States)

    Kezhu, Wang; Pan, Xu; Cong, Lu; Liming, Dong; Beiyue, Zhang; Jingwei, Lu; Yanyan, Yang; Xinmin, Liu

    2017-01-01

    Ginsenoside Rg1 is one of the major active ingredients of Panax ginseng and has showed notable improving learning and memory effects in several behavioral tasks, such as water maze, shuttle-box, and step-through, based on avoidance. However, there was no report about the role of Rg1 on the performance of reward-directed instrumental conditioning, which could reflect the adaptive capacity to ever-changing environments. Thus, in this study, the reward devaluation test and conditional visual discrimination task were conducted to study the ameliorating effects of Rg1 on cognitive deficits, especially the loss of adaptation capacity in chronic restraint stress (CRS) rat model. Our results showed that rat subjected to CRS became insensitive to the changes in outcome value, and it significantly harmed the rat's performance in conditional visual discrimination task. Moreover, the levels of BDNF, TrkB, and Erk phosphorylation were decreased in the prefrontal cortex of CRS rats. However, these changes were effectively reversed by Rg1 (5 and 10 mg/kg, i.p.). Therefore, it demonstrated that Rg1 has a good ability to improve learning and memory and also ameliorate impaired adaptive capacity induced by CRS. This amelioration effect of Rg1 might be mediated partially by BDNF/TrkB/Erk pathway in prefrontal cortex. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  19. Rg1 inhibits high glucose-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway.

    Science.gov (United States)

    Xue, Li-Ping; Fu, Xiao-Lin; Hu, Min; Zhang, Li-Wei; Li, Ya-Di; Peng, Ya-Li; Ding, Peng

    2018-07-02

    Recent study has showed that Ginsenoside Rg1, the mian active compound of Panax ginseng, could ameliorate oxidative stress and myocardial apoptosis in diabetes mellitus. However, the roles and mechanisms of Rg1 in proliferative diabetic retinopathy (PDR) are still unclear. In the present study, we aimed to investigate the effects of Rg1 on mesenchymal activation of high-glucose (HG) cultured müller cells. High glucose conditions up-regulate MMP-2, MMP-9 and down-regulate TIMP-2, and promote mesenchymal activation in Müller cells. And Rg1 inhibits the HG-induced mesenchymal activation and HG-increased MMP-2 and MMP-9 and HG-decreased TIMP-2 in Müller cells. HG up-regulates Zeb1 and lncRNA RP11-982M15.8, and down-regulates miR-2113, and Rg1 inhibits these effects of HG. Both inhibition of miR-2113 and over-expression of RP11-982M15.8 significantly restored the HG induced mesenchymal activasion. Taken together, our findings suggested that Rg1 inhibited HG-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway. Copyright © 2018. Published by Elsevier Inc.

  20. Ginsenoside Rg1 protects rat bone marrow mesenchymal stem cells against ischemia induced apoptosis through miR-494-3p and ROCK-1.

    Science.gov (United States)

    Zheng, Hui-Zhen; Fu, Xue-Kun; Shang, Jiu-Long; Lu, Rong-Xi; Ou, Yong-Fang; Chen, Chun-Ling

    2018-03-05

    This study aimed to verify the cytoprotective effect of ginsenoside Rg1 in vivo, and to elucidate the mechanism of Rg1 in the ischemic microenvironment. Male rat bone marrow mesenchymal stem cells (rBMSCs) or rBMSCs treated with Rg1 were injected into ischemic region of the arterial embolism hind limb in female rats. Behavioral and histological data, obtained one-week post injection, showed that rBMSCs with Rg1 could improve the survival rate of BMSCs and enhance the therapeutic effects. rBMSCs treated with hypoxia and serum deprivation for 24h (H/SD-rBMSCs) showed the up-regulated expression of ras homolog family member A (RhoA), Rho associated coiled-coil containing protein kinase 1 (ROCK-1), myosin light chain 2 (MLC-2), Bcl2 associated agonist of cell death (Bad) and Bcl2 associated X, apoptosis regulator (Bax); while the expression of miR-148b-3p, miR-148b-5p and miR-494-3p was down-regulated. H/SD with Rg1 treatment (H/SD+Rg1-rBMSCs) inhibited the expression of ROCK-1, MLC-2, Bad and Bax, increased the expression of Bcl-2, miR-494-3p. After ROCK-1 knockout, the expression of Bad and Bax were downregulated and Bcl-2 upregulated, but Rg1 no longer altered their expression. Mir-494-3p functional study established that miR-494-3 mimic downregulated and miR-494-3 inhibitor upregulated ROCK-1 gene expression, Rg1 did not have the ability to change the ROCK gene expression after loss of function of miR-494-3p. Also, the function loss of mir-494-3p promoted apoptosis; otherwise reduced apoptosis. The anti-apoptotic effect of Rg1 disappeared after mir-494-3p loss or gain function. In conclusion, Ginsenoside Rg1 has shown to have protective effects on ischemic-induced rBMSCs apoptosis through mir-494-3p→ROCK-1→Bcl-2 signaling pathway. Copyright © 2018. Published by Elsevier B.V.

  1. Caspase-Mediated Anti-Apoptotic Effect of Ginsenoside Rg5, a Main Rare Ginsenoside, on Acetaminophen-Induced Hepatotoxicity in Mice.

    Science.gov (United States)

    Wang, Zi; Hu, Jun-Nan; Yan, Meng-Han; Xing, Jing-Jing; Liu, Wen-Cong; Li, Wei

    2017-10-25

    Frequent overdose of acetaminophen (APAP) is one of the most common and important incentives of acute hepatotoxicity. Prior to this work, our research group confirmed that black ginseng (Panax ginseng, BG) showed powerful protective effects on APAP-induced ALI. However, it is not clear which kind of individual ginsenoside from BG plays such a liver protection effect. The objective of the current investigation was to evaluate whether ginsenoside Rg5 (G-Rg5) protected against APAP-induced hepatotoxicity and the involved action mechanisms. Mice were administrated with G-Rg5 at two dosages of 10 or 20 mg/kg for 7 consecutive days. After the last treatment, all of the animals that received a single intraperitoneal injection of APAP (250 mg/kg) showed severe liver toxicity after 24 h, and the liver protection effects of G-Rg5 were examined. The results clearly indicated that pretreatment with G-Rg5 remarkably inhibited the production of serum tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) compared with the APAP group. Meanwhile, G-Rg5 decreased the hepatic malondialdehyde (MDA) content, the protein expression levels of 4-hydroxynonenal (4-HNE) and cytochrome P450 2E1 (CYP2E1) in the liver tissues. G-Rg5 decreased APAP caused the hepatic overexpression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Furthermore, analysis of immunohistochemistry and Western blotting also indicated that G-Rg5 pretreatment inhibited activation of apoptotic pathways mainly via increasing the expression of Bcl-2 protein, decreasing the expression of Bax protein, proliferating cell nuclear antigen (PCNA), cytochrome c, caspase-3, caspase-8, and caspase-9. Liver histopathological observation provided further evidence that pretreatment with G-Rg5 could significantly inhibit hepatocyte necrosis, inflammatory cell infiltration, and apoptosis caused by APAP. In conclusion, the present study clearly demonstrates that G-Rg5 exerts a liver protection effect against

  2. 95. German Roentgen congress and 7. joint congress of the DRG and OeRG. Program with abstracts; 95. Deutscher Roentgenkongress und 7. Gemeinsamer Kongress von DRG und OeRG. Vollstaendiges Programm mit Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    Diederich, Stefan; Lammer, Johannes (eds.)

    2014-05-15

    The volume contains the program and the abstracts of the 95th German Roentgen congress and the 7th joint congress of the DRG and OeRG. The radiological focal points of the congress were thorax radiology (pneumology: lung fibrosis, emphysema); oncological radiology: skeletal carcinoma, lung carcinoma, kidneys, lung metastases, primary liver carcinoma, liver metastases. Further topics included radiology in hospitals and medical centers, tele-radiology, ambulant health care, legal issues, financial accounting and management issues. Several courses an specific radiological issues and radiological techniques were offered, including radiation protection and legal aspects.

  3. Síndrome metabólico en empleados en la Argentina Metabolic syndrome in employees in Argentina

    Directory of Open Access Journals (Sweden)

    Raúl I Coniglio

    2009-04-01

    Full Text Available La detección del síndrome metabólico (SM es útil para identificar individuos en riesgo para la diabetes mellitus tipo 2 y la enfermedad coronaria. Los objetivos de este trabajo fueron describir la prevalencia del síndrome metabólico (SM en empleados de 40 a 65 años utilizando diferentes definiciones y analizar la relación con el nivel de educación y el sexo, mediante una investigación observacional, transversal y multicéntrica en diferentes regiones de Argentina. Se compararon las definiciones de la Federación Internacional de Diabetes, la Asociación Americana de Cardiología/Instituto Nacional del Corazón, Pulmones y Sangre de EE.UU. y el Tercer Panel de Expertos del Programa Nacional de Educación para el Colesterol de EE.UU. Cumplimentaron el protocolo 2806 casos. La prevalencia fue: 0.31, 0.30 y 0.26 respectivamente y mayor en varones (p=0.0000. No se observaron diferencias significativas entre sexos en el grupo de 60 a 65 años de edad. Luego de ajustar por edad, sexo, actividad física, historia familiar de diabetes y menopausia, las mujeres con bajo nivel educativo (The detection of metabolic syndrome (MS is use ful for identifying individuals at risk for type 2 diabetes and coronary heart disease. The objectives of the study were to describe the prevalence of MS in employees 40-65 years old, utilizing different definitions and to analyze the relation with educational level and gender by means of cross-sectional and multicenter study of different regions of Argentina. Compared MS definitions were: International Diabetes Federation, American Heart Association/National Heart, Lung and Blood Institute and National Cholesterol Education Program - Adults Treatment Panel III. Fulfilled the protocol 2806 cases. It was observed a prevalence of 0.31, 0.30 and 0.26 respectively, more frequent in men (p = 0.0000. There was no significant difference between sexes in the group 60 to 65 years old. After adjusting to age, sex, physical

  4. Expressão hormonal e metabólica dos carcinomas broncogénicos

    Directory of Open Access Journals (Sweden)

    Cyro Teixeira da Silva Junior

    2003-03-01

    Full Text Available RESUMO: No presente artigo de revisão os autores abordam um tema não levado em consideração pela maioria dos clínicos e pneumologistas, que são as manifestações endócrino-metabólicas do carcinoma broncogénico. As principais manifestações encontradas na literatura são as síndromas para neoplásicas (síndroma de secreção inapropriada da hormona antidiurética, alterações no metabolismo do cálcio, síndroma carcinóide e de Cushing. A osteoartropatia hipertrófica também é revista.REV PORT PNEUMOL 2003; IX (2: 109-115 ABSTRACT: In the present paper, the authors reviews the endocrine and metabolic manifestations in bronchogenic carcinoma. Disorders as paraneoplastic syndromes (hypercalcemia, syndrome of inappropriate antidiuretic hormone, carcinoide and Cushing’s syndromes and others endocrinopathies are discussed. Hypertrophic pulmonary osteoarthropathy is briefly rewiewed.REV PORT PNEUMOL 2003; IX (2: 109-115 Palavras-chave: carcinoma broncogénico, hormonas, síndromas paraneoplásicas, marcadores tumorais, Key-words: bronchogenic carcinoma, hormones, paraneoplastic syndromes, tumor markers

  5. Obesidad y síndrome metabólico en niños y adolescentes

    Directory of Open Access Journals (Sweden)

    Andrés Rosende

    2013-10-01

    Full Text Available La obesidad y el síndrome metabólico se encuentran íntimamente relacionados con los casos de enfermedad cardiovascular; son considerados habitualmente como propios de la población adulta aunque se observan con una frecuencia creciente en niños y adolescentes. Existe evidencia de que las lesiones ateroescleróticas se presentan con más frecuencia en jóvenes obesos. Los factores involucrados en esta pandemia son múltiples e incluyen desde variables genético-biológicas hasta cambios culturales. La familia y el entorno en el que el niño se desarrolla juegan un papel fundamental en la adopción de los hábitos relacionados con la alimentación y la actividad física. Esta problemática no respeta fronteras ni culturas sino que todos los países están siendo afectados, más aún aquellos de ingresos medios. El papel que le cabe al Estado y a la sociedad en su conjunto se orienta a modificar este medio ambiente. La restricción en las ventas de alimentos poco saludables y la lucha contra el sedentarismo son políticas que urgen ser aplicadas. El impacto que estos trastornos tendrán en términos de enfermedad cardiovascular, no ha cobrado aún su verdadera dimensión.

  6. Síndrome metabólica e menopausa: estudo transversal em ambulatório de ginecologia Síndrome metabólico y menopausia: estudio transversal en ambulatorio de ginecología Metabolic syndrome and menopause: cross-sectional study in gynecology clinic

    Directory of Open Access Journals (Sweden)

    José Albuquerque de Figueiredo Neto

    2010-09-01

    Full Text Available FUNDAMENTO: No Brasil, são escassos os estudos sobre síndrome metabólica na população geral, mais raros são os que a correlacionam ao climatério. OBJETIVO: Determinar a prevalência da síndrome metabólica e seus componentes em mulheres climatéricas. MÉTODOS: Estudo transversal com 323 mulheres climatéricas, divididas em dois grupos: pré e pós-menopausadas. Foram avaliadas para presença de síndrome metabólica, segundo os critérios do National Cholesterol Education Program's (NCEP e da International Diabetes Federation (IDF. Foi verificada a associação entre as variáveis estudadas e a síndrome metabólica por meio de análise uni e multivariada. Um p-valor FUNDAMENTO: En Brasil, son raros los estudios sobre síndrome metabólico en la población general, más raros son los que la correlacionan al climaterio. OBJETIVO: Determinar la prevalencia del síndrome metabólico y sus componentes en mujeres climatéricas. MÉTODOS: Estudio transversal con 323 mujeres climatéricas, divididas en dos grupos: pre y posmenopáusicas. Se evaluaron para presencia de síndrome metabólico, según los criterios del NCEP y de la IDF. Se verificó la asociación entre las variables estudiadas y el síndrome metabólico por medio de análisis uni e multivariado. Un p-valor BACKGROUND: In Brazil, there are few studies of the metabolic syndrome in the general population, and even fewer studies that establish a correlation between metabolic syndrome and climacteric. OBJECTIVE: To determine the prevalence of metabolic syndrome and its components among climacteric women. METHODS: Cross-sectional study with 323 climacteric women, divided into two groups: pre and post-menopausal. They were examined for the presence of metabolic syndrome, according to the criteria of National Cholesterol Education Program's (NCEP and International Diabetes Federation (IDF. It was possible to notice the association between the variables under study and the metabolic

  7. Prevalencia del síndrome metabólico en pobladores peruanos por debajo de 1 000 y por encima de los 3 000 msnm

    Directory of Open Access Journals (Sweden)

    Jaime Pajuelo

    2012-04-01

    Full Text Available Objetivos: Determinar y comparar la prevalencia del síndrome metabólico y de sus diferentes componentes en población adulta peruana que habita por debajo de 1 000 y por encima de 3 000 msnm. Diseño: Análisis de los datos de la Encuesta Nacional de Indicadores Nutricionales, Bioquímicos, Socioeconómicos y Culturales Relacionados a las Enfermedades Crónicas Degenerativas 2006. Institución: Instituto Nacional de Salud Centro Nacional de Alimentación y Nutrición, Ministerio de Salud, Lima, Perú. Participantes: Personas mayores de 20 años. Métodos: Se incluyó 3 384 personas mayores de 20 años, 2 425 que habitaban por debajo de 1 000 (nivel-I y 959 por encima de los 3 000 msnm (nivel-II. El síndrome metabólico fue definido de acuerdo a los criterios del National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III. Principales medidas de resultados: Prevalencia del síndrome metabólico y sus componentes. Resultados: La prevalencia de síndrome metabólico fue significativamente mayor en el nivel-I (19,7% que en el nivel-II (10,2%, p<0,001. En varones, la prevalencia fue 9,2% en el nivel-I y 5,1% en el nivel-II. En mujeres fue 29,9% en el nivel-I comparado con 15,2% en el nivel-II. La obesidad central (35,5% vs. 21,1%, elevación de presión arterial (20,9% vs. 15,0%, hiperglicemia (3,9% vs. 1,7%, hipertrigliceridemia (31,3% vs. 25,7% y concentraciones bajas de HDLc (57,4% vs. 52,5% fueron significativamente más prevalentes en el nivel-I comparado con el nivel-II (p<0,05. Los componentes del síndrome metabólico más frecuentes en los varones fueron la hipertrigliceridemia y el HDLc bajo, mientras que en las mujeres fueron la obesidad central y el HDLc disminuido. Conclusiones: El síndrome metabólico fue significativamente más prevalente en la población por debajo de los 1 000 msnm; esto podría ser resultado de la transición nutricional, demográfica y socioeconómica en esta área. Las mujeres fueron m

  8. Endophytic Bacterium Pseudomonas fluorescens RG11 May Transform Tryptophan to Melatonin and Promote Endogenous Melatonin Levels in the Roots of Four Grape Cultivars.

    Science.gov (United States)

    Ma, Yaner; Jiao, Jian; Fan, Xiucai; Sun, Haisheng; Zhang, Ying; Jiang, Jianfu; Liu, Chonghuai

    2016-01-01

    Endophytes have been verified to synthesize melatonin in vitro and promote abiotic stress-induced production of endogenous melatonin in grape ( Vitis vinifera L.) roots. This study aimed to further characterize the biotransformation of tryptophan to melatonin in the endophytic bacterium Pseudomonas fluorescens RG11 and to investigate its capacity for enhancing endogenous melatonin levels in the roots of different grape cultivars. Using ultra performance liquid chromatography-tandem mass spectrometry combined with 15N double-labeled L -tryptophan as the precursor for melatonin, we detected isotope-labeled 5-hydroxytryptophan, serotonin, N -acetylserotonin, and melatonin, but tryptamine was not detected during the in vitro incubation of P. fluorescens RG11. Furthermore, the production capacity of these four compounds peaked during the exponential growth phase. RG11 colonization increased the endogenous levels of 5-hydroxytryptophan, N -acetylserotonin, and melatonin, but reduced those of tryptamine and serotonin, in the roots of the Red Globe grape cultivar under salt stress conditions. Quantitative real-time PCR revealed that RG11 reduced the transcription of grapevine tryptophan decarboxylase and serotonin N -acetyltransferase genes when compared to the un-inoculated control. These results correlated with decreased reactive oxygen species bursts and cell damage, which were alleviated by RG11 colonization under salt stress conditions. Additionally, RG11 promoted plant growth and enhanced the levels of endogenous melatonin in different grape cultivars. Intraspecific variation in the levels of melatonin precursors was found among four grape cultivars, and the associated root crude extracts appeared to significantly induce RG11 melatonin biosynthesis in vitro . Overall, this study provides useful information that enhances the existing knowledge of a potential melatonin synthesis pathway in rhizobacteria, and it reveals plant-rhizobacterium interactions that affect

  9. Anti-inflammatory function of ginsenoside Rg1 on alcoholic hepatitis through glucocorticoid receptor related nuclear factor-kappa B pathway.

    Science.gov (United States)

    Gao, Yan; Chu, Shifeng; Li, Jingwei; Li, Jianping; Zhang, Zhao; Xia, Congyuan; Heng, Yang; Zhang, Meijin; Hu, Jinfeng; Wei, Guining; Li, Yueting; Chen, Naihong

    2015-09-15

    Ginseng is the dried root of Panax ginseng C.A. Mayer. Since ancient times, ginseng has been used as one kind of treatment drug or tonic in China and even other eastern countries like Korea and Japan. Pharmacological active chemical ingredients and its extract of ginseng are a mixture of triterpenoid saponins, collectively called ginsenosides. Among them, ginsenoside Rg1 is the most pharmacological active one. Based on prior experimental results and the understanding of alcoholic hepatitis, the major aim of this study is to investigate whether Rg1 is beneficial in a rodent model mimic alcoholic hepatic injury associated with binge drinking and explore the underlying possible mechanisms. C57BL/6 mice were given oral consumption of 6g/kg alcohol 1h after treated with Rg1 (10, 20 and 40mg/kg) or dexamethasone (1mg/kg) for 9 consecutive days. Biochemical analyses were performed and liver fragments were processed for microscopy, immunohistochemistry and western blot analysis. According to our data, Rg1 treatment significantly reversed the high mortality rate induced by alcohol consumption and also alleviated liver impairment as evidenced by the decrease of serum parameters. Meanwhile, histological and ultrastructural analysis of alcoholic groups showed hepatocellular impairment but restored in Rg1-treated groups. Overproductive inflammatory cytokines were also suppressed by Rg1 in alcohol-intoxicated mouse livers. In addition, changes of GR related NF-κB pathway, including phospho-IκB-α, were also modulated to normal levels. This study demonstrates that Rg1 might promote GR mediating the repression of NF-κB and inhibit the inflammatory reactions in alcoholic hepatitis. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  10. Métodos para estudo das respostas metabólicas de cães e gatos a diferentes alimentos Methods of study of metabolic responses of dogs and cats to different foods

    Directory of Open Access Journals (Sweden)

    Aulus Cavalieri Carciofi

    2007-07-01

    Full Text Available Durante o processo de assimilação e uso dos alimentos, diferentes respostas metabólicas podem ser desenvolvidas pelo organismo dos animais. Estas respostas são fruto da integração de mecanismos complexos, que envolvem os sistemas neuro-endócrino e o funcionamento dos órgãos, sendo influenciadas pela dieta, espécie animal, idade, condição fisiológica e composição corporal. Este trabalho enfoca a importância fisiológica e os métodos de estudo das respostas pós-prandiais aos carboidratos, bem como as alterações fisiológicas conseqüentes ao balanço eletrolítico da dieta. A quantidade, estrutura química e processamento industrial do amido determinam boa parte da resposta pós-prandial de glicose e insulina de cães. Em gatos, outros mecanismos parecem ser mais importantes, como a ingestão de aminoácidos. A fibra alimentar também altera a resposta pós-prandial ao alimento, devendo ser consideradas sua quantidade, solubilidade e fermentabilidade no desenvolvimento das dietas. Os métodos de estudo destas respostas incluem avaliação das respostas glicêmica e insulínca pós-prandiais, teste endovenoso de tolerância à glicose e à arginina. O clâmp euglicêmico apresenta-se também como ferramenta de estudo, no entanto revela informações mais relacionadas ao animal do que à dieta. A compreensão do conjunto de alterações metabólicas aos carboidratos é importante no estudo do controle da saciedade, composição corporal e inúmeras doenças degenerativas e endócrinas. A concentração e relação entre os macro-elementos da dieta (Na, Cl, K, P, Ca, Mg e S e dos aminoácidos sulfurados (metionina, cistina e taurina interferem em inúmeras funções orgânicas, como a cardiovascular, neuromuscular, metabolismo ósseo, renal e pulmonar, refletindo-se no equilíbrio hidro-eletrolítico e ácido-básico orgânicos. De importância prática para cães e gatos encontram-se a relação destes nutrientes com cardiopatias

  11. Development of a framework for resilience measurement: Suggestion of fuzzy Resilience Grade (RG) and fuzzy Resilience Early Warning Grade (REWG).

    Science.gov (United States)

    Omidvar, Mohsen; Mazloumi, Adel; Mohammad Fam, Iraj; Nirumand, Fereshteh

    2017-01-01

    Resilience engineering (RE) can be an alternative technique to the traditional risk assessment and management techniques, to predict and manage safety conditions of modern socio-technical organizations. While traditional risk management approaches are retrospective and highlight error calculation and computation of malfunction possibilities, resilience engineering seeks ways to improve capacity at all levels of organizations in order to build strong yet flexible processes. Considering the resilience potential measurement as a concern in complex working systems, the aim of this study was to quantify the resilience by the help of fuzzy sets and Multi-Criteria Decision-Making (MCDM) techniques. In this paper, we adopted the fuzzy analytic hierarchy process (FAHP) method to measure resilience in a gas refinery plant. A resilience assessment framework containing six indicators, each with its own sub-indicators, was constructed. Then, the fuzzy weights of the indicators and the sub-indicators were derived from pair-wise comparisons conducted by experts. The fuzzy evaluating vectors of the indicators and the sub-indicators computed according to the initial assessment data. Finally, the Comprehensive Resilience Index (CoRI), Resilience Grade (RG), and Resilience Early Warning Grade (REWG) were established. To demonstrate the applicability of the proposed method, an illustrative example in a gas refinery complex (an instance of socio-technical systems) was provided. CoRI of the refinery ranked as "III". In addition, for the six main indicators, RG and REWG ranked as "III" and "NEWZ", respectively, except for C3, in which RG ranked as "II", and REWG ranked as "OEWZ". The results revealed the engineering practicability and usefulness of the proposed method in resilience evaluation of socio-technical systems.

  12. Morfologia dos órgãos genitais externos do macho de cutia (Dasyprocta aguti. Linnaeus, 1766

    Directory of Open Access Journals (Sweden)

    Danilo José Ayres de Menezes

    2003-01-01

    Full Text Available Foram utilizados vinte e três cutias (D. aguti, machos, adultos, adquiridos em criatórios científicos, legalmente licenciados pelo IBAMA, da Universidade Federal do Piauí - UFPI, Teresina, PI e da Escola Superior Agrícola de Mossoró - ESAM, Mossoró, RN, com o objetivo de descrever morfologicamente os órgãos genitais masculinos externos, abordando aspectos histológicos e macroscópicos destes órgãos. Foi realizada a dissecação dos órgãos genitais externos dos espécimes. Fragmentos foram coletados e submetidos ao processamento histológico de rotina para inclusão em parafina, os quais foram cortados e corados pelos métodos de H/E e Tricômico de Masson e observados em microscópio de luz. Os resultados mostraram que o pênis destes animais possui características do tipo fibrocavernoso, com grande quantidade de estruturas queratinizadas no seu ápice. No terço médio do pênis da cutia foi evidenciado uma flexura peniana em forma de "U" deitado; a glande do pênis encontra-se revestida por uma epiderme queratinizada contendo espículas córneas; um osso peniano substituindo o corpo cavernoso e um saco suburetral ventral, com dois longos esporões córneos no seu interior, estão presentes no pênis desses espécimes. Na região escrotal não se observa limites externos nítidos do escroto. Dorsalmente, abaixo da lâmina interna do prepúcio, próximo ao óstio prepucial, verifica-se um par de glândulas prepuciais.

  13. Dose- and time-dependent effects of phenobarbital on gene expression profiling in human hepatoma HepaRG cells

    International Nuclear Information System (INIS)

    Lambert, Carine B.; Spire, Catherine; Claude, Nancy; Guillouzo, Andre

    2009-01-01

    Phenobarbital (PB) induces or represses a wide spectrum of genes in rodent liver. Much less is known about its effects in human liver. We used pangenomic cDNA microarrays to analyze concentration- and time-dependent gene expression profile changes induced by PB in the well-differentiated human HepaRG cell line. Changes in gene expression profiles clustered at specific concentration ranges and treatment times. The number of correctly annotated genes significantly modulated by at least three different PB concentration ranges (spanning 0.5 to 3.2 mM) at 20 h exposure amounted to 77 and 128 genes (p ≤ 0.01) at 2- and 1.8-fold filter changes, respectively. At low concentrations (0.5 and 1 mM), PB-responsive genes included the well-recognized CAR- and PXR-dependent responsive cytochromes P450 (CYP2B6, CYP3A4), sulfotransferase 2A1 and plasma transporters (ABCB1, ABCC2), as well as a number of genes critically involved in various metabolic pathways, including lipid (CYP4A11, CYP4F3), vitamin D (CYP24A1) and bile (CYP7A1 and CYP8B1) metabolism. At concentrations of 3.2 mM or higher after 20 h, and especially 48 h, increased cytotoxic effects were associated with disregulation of numerous genes related to oxidative stress, DNA repair and apoptosis. Primary human hepatocyte cultures were also exposed to 1 and 3.2 mM PB for 20 h and the changes were comparable to those found in HepaRG cells treated under the same conditions. Taken altogether, our data provide further evidence that HepaRG cells closely resemble primary human hepatocytes and provide new information on the effects of PB in human liver. These data also emphasize the importance of investigating dose- and time-dependent effects of chemicals when using toxicogenomic approaches

  14. Nonviral gene therapy in vivo with PAM-RG4/apoptin as a potential brain tumor therapeutic.

    Science.gov (United States)

    An, Songhie; Nam, Kihoon; Choi, Sunghyun; Bai, Cheng Z; Lee, Yan; Park, Jong-Sang

    2013-01-01

    Glioma is still one of the most complicated forms of brain tumor to remove completely due to its location and the lack of an efficient means to specifically eliminate tumor cells. For these reasons, this study has examined the effectiveness of a nonviral gene therapy approach utilizing a tumor-selective killer gene on a brain tumor xenograft model. The therapeutic apoptin gene was recombined into the JDK plasmid and delivered into human brain tumor cells (U87MG) by using a polyamidoamine dendrimer with an arginine surface (PAM-RG4). Studies in vitro showed that the PAM-RG4/apoptin plasmid polyplex exhibited a particularly high transfection activity of .40%. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, 4',6-Diamidino-2-phenylindole (DAPI) TUNEL assay, DAPI staining, and caspase-3 activity assay verified that the tumor cells had undergone apoptosis induced by apoptin. For in vivo studies, the polyplex was injected into tumors, which were induced by injecting U87MG cells intradermally into nude mice. Based on hematoxylin and eosin staining, epidermal growth factor receptor immunohistochemistry results and tumor volume measurement results, tumor growth was effectively inhibited and no specific edema, irritation, or other harm to the skin was observed after polyplex injection. The in vivo expression of apoptin and the induction of apoptosis were verified by reverse-transcription polymerase chain reaction analysis, TUNEL assay, and DAPI staining. The PAM-RG4/apoptin gene polyplex is a strong candidate for brain tumor therapeutics because of the synergistic effect of the carrier's high transfection efficiency (35%-40%) in glioma cells and the selective apoptosis-inducing activity of apoptin in tumor cells.

  15. Adipocinas y síndrome metabólico: múltiples facetas de un proceso fisiopatológico complejo

    Directory of Open Access Journals (Sweden)

    Julio C. Sánchez N., MD., PhD

    2010-07-01

    Full Text Available Debido a la alta morbimortalidad de las enfermedades cardiovasculares y a su relación con trastornos de base como la obesidad y el síndrome metabólico, es crucial entender cuáles son los mecanismos y procesos que desencadenan la alteración del metabolismo y a su vez la generación de dichas enfermedades. En tal sentido, el tejido adiposo y el adipocito tienen un papel fundamental en este proceso, mediante la producción de múltiples adipocinas, algunas clásicas y otras de reciente descripción, pero que hasta ahora empieza a dilucidarse en medio del complejo panorama de interacciones fisiopatológicas conducentes al desarrollo de resistencia a la insulina y del complejo desequilibrio metabólico que conlleva un sinnúmero de complicaciones clínicas. Un grupo de estas adipocinas tiene claros efectos proinflamatorios, mientras que otras pueden clasificarse como anti-inflamatorias, las cuales contrarrestan en cierta medida y hasta cierto punto las acciones de las otras. Cuando esta homeostasis se rompe, la cascada de inflamación crónica allí originada desencadena resistencia a la insulina y se inicia el desarrollo del síndrome metabólico a partir de la obesidad, que a su vez genera alteraciones de la respuesta del adipocito a diferentes estímulos. Esto, sumado a los efectos de otros elementos, configura un complejo cuadro de factores que es necesario tener en cuenta para el abordaje correcto de la obesidad y sus patologías asociadas.

  16. rg Matthias Determann, Historiography in Saudi Arabia: Globalization and the State in the Middle East

    OpenAIRE

    Pétriat, Philippe

    2016-01-01

    It is a widely held view that Saudi Arabia’s history is recent, that its main elements are oil, Salafism and sectarianism, and that this recent history has been written mostly by non-Saudi scholars. In a stimulating in-depth analysis of Saudi historiography on Saudi Arabia, Jörg Matthias Determann delves into the very writing of history. He draws on an impressive range of written (including Saudi and non-Saudi historical works, dissertations, articles and textbooks) as well as oral sources. A...

  17. 95. German Roentgen congress and 7. joint congress of the DRG and OeRG. Program with abstracts

    International Nuclear Information System (INIS)

    Diederich, Stefan; Lammer, Johannes

    2014-01-01

    The volume contains the program and the abstracts of the 95th German Roentgen congress and the 7th joint congress of the DRG and OeRG. The radiological focal points of the congress were thorax radiology (pneumology: lung fibrosis, emphysema); oncological radiology: skeletal carcinoma, lung carcinoma, kidneys, lung metastases, primary liver carcinoma, liver metastases. Further topics included radiology in hospitals and medical centers, tele-radiology, ambulant health care, legal issues, financial accounting and management issues. Several courses an specific radiological issues and radiological techniques were offered, including radiation protection and legal aspects.

  18. Nonviral gene therapy in vivo with PAM-RG4/apoptin as a potential brain tumor therapeutic

    Directory of Open Access Journals (Sweden)

    An S

    2013-02-01

    Full Text Available Songhie An,* Kihoon Nam,* Sunghyun Choi, Cheng Z Bai, Yan Lee, Jong-Sang ParkDepartment of Chemistry, Seoul National University, Seoul, Republic of Korea*These authors contributed equally to this workBackground: Glioma is still one of the most complicated forms of brain tumor to remove completely due to its location and the lack of an efficient means to specifically eliminate tumor cells. For these reasons, this study has examined the effectiveness of a nonviral gene therapy approach utilizing a tumor-selective killer gene on a brain tumor xenograft model.Methods and results: The therapeutic apoptin gene was recombined into the JDK plasmid and delivered into human brain tumor cells (U87MG by using a polyamidoamine dendrimer with an arginine surface (PAM-RG4. Studies in vitro showed that the PAM-RG4/apoptin plasmid polyplex exhibited a particularly high transfection activity of >40%. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL assay, 4´,6-Diamidino-2-phenylindole (DAPI TUNEL assay, DAPI staining, and caspase-3 activity assay verified that the tumor cells had undergone apoptosis induced by apoptin. For in vivo studies, the polyplex was injected into tumors, which were induced by injecting U87MG cells intradermally into nude mice. Based on hematoxylin and eosin staining, epidermal growth factor receptor immunohistochemistry results and tumor volume measurement results, tumor growth was effectively inhibited and no specific edema, irritation, or other harm to the skin was observed after polyplex injection. The in vivo expression of apoptin and the induction of apoptosis were verified by reverse-transcription polymerase chain reaction analysis, TUNEL assay, and DAPI staining.Conclusion: The PAM-RG4/apoptin gene polyplex is a strong candidate for brain tumor therapeutics because of the synergistic effect of the carrier's high transfection efficiency (35%–40% in glioma cells and the selective apoptosis-inducing activity of

  19. Processo de transporte de órgãos para transplante na Cidade de São Paulo

    OpenAIRE

    Adriana Roberta Lira; Elton Lima dos Reis; Marcus Vinícius de Oliveira Santos

    2016-01-01

    O trabalho da Polícia Militar do Estado de São Paulo, em favor da sociedade completa 185 anos de existência. É um trabalho amplo e digno. Uma parcela desse trabalho apoia-se no Grupamento Aéreo da Polícia Militar do Estado de São Paulo, guarnição direcionada especificamente à atividade de transportar órgãos para transplante. Nesse sentido, o presente artigo, objetiva avaliar essa atividade, baseando-se em pesquisa realizada junto às Instituições e profissionais responsáveis pela captação e o ...

  20. Obesidad y riesgo de síndrome metabólico en estudiantes de posgrado de Veracruz, México

    Directory of Open Access Journals (Sweden)

    Martha Gabriela Campos-Mondragón

    2016-01-01

    Full Text Available Introducción: Debido a que la salud de los estudiantes influye en su rendimiento académico y eficiencia terminal, y la obesidad aumenta en los adultos, se planteó analizar las variables antropométricas y ambientales asociadas al riesgo de síndrome metabólico en estudiantes de posgrado.Material y Métodos: Estudio transversal en 52 estudiantes mediante muestreo intencional no probabilístico. Se evaluaron peso, estatura e índice de masa corporal. Según la circunferencia de cintura, relación cintura-cadera y grasa corporal, se identificó el riesgo de síndrome metabólico. Se registraron antecedentes familiares, personales, peso al nacer, ingesta semanal de alimentos, alcohol, tabaco y café, nivel de actividad física, depresión y estrés. Se analizaron diferencias entre medias, correlaciones y modelos de regresión lineal.Resultados: El peso (74,8±13,9; p=0,0024, la circunferencia de cintura (87,6±9,0; p=0,0044 y la relación cintura-cadera (0,9±0,0; p=0,0000 fueron superiores en los hombres y la grasa corporal en las mujeres (35,4±4,5; p=0,0000. Según la circunferencia de cintura y la grasa corporal, el 34% y el 87% respectivamente mostraron riesgo de síndrome metabólico, en su mayoría mujeres. El modelo de regresión lineal para grasa corporal incluyó sexo (b=9,5; p=0,0000 y estrés leve (b=4,7; p=0,0410. El modelo para índice de masa corporal incluyó circunferencia de cadera, circunferencia de cintura, depresión y estrés severo (b=1,61; p=0,0486. Más del 50% mostraron un consumo nulo de leche, nueces y leguminosas.Conclusiones: Las variables asociadas a los indicadores de mayor riesgo de síndrome metabólico fueron el sexo y el estrés. Se evidenciaron hábitos de alimentación deficientes.

  1. Prevalencia y Factores de Riesgo de Síndrome Metabólico en Población adulta del Departamento de Lambayeque, Perú - 2004

    Directory of Open Access Journals (Sweden)

    Víctor Soto C

    2005-10-01

    Full Text Available Objetivos: Determinar la prevalencia y factores de riesgo de síndrome metabólico en la población adulta del departamento de Lambayeque en el año 2004. Materiales y métodos: Estudio descriptivo, transversal y prospectivo, realizado en el departamento de Lambayeque; se incluyeron 1000 personas entre 30 y 70 años de edad mediante un muestreo probabilístico polietápico; se realizaron mediciones antropométricas y de presión arterial, así como análisis de glicemia, colesterol total, triglicéridos y HDL colesterol. Se usaron las definiciones de síndrome metabólico de la ATP III y de la Oficina Internacional de Información en Lípidos Latinoamérica (ILIBLA. Resultados: La prevalencia del síndrome metabólico según criterios ATP III es 28,3% (IC95: 25,4-37,1 y según ILIBLA es de 33,2% (IC95: 28,1-38,3, la prevalencia de hipertensión arterial es 17,8%, diabetes mellitus tipo 2 de 3,3%, hipercolesterolemia 47,3%, hipertrigliceridemia 43,4%, HDL bajo 56,3%. La prevalencia de obesidad (índice de masa corporal e» 30 es de 30,2%, la obesidad central según circunferencia abdominal (ATP III es 44,4% y según índice cintura cadera (ILIBLA 63,3%. No se encontró asociación entre el SM y el consumo de pescado, dieta hipercalórica, actividad física, tabaco, alcohol, ocupación, sólo con el sexo masculino y la edad e»50 años. Conclusiones: Más de uno de cada cuatro adultos en el departamento de Lambayeque presenta síndrome metabólico, la proporción se incrementa conforme avanza la edad y es predominante en el sexo masculino según criterios ATP III.

  2. Patrones de alimentarios, adiposidad y alteraciones metabólicas en niños y adolescentes en España

    OpenAIRE

    Vázquez Cobela, Rocío

    2016-01-01

    La OMS reconoce que la epidemia de la obesidad se debe a una combinación de factores prenatales, genéticos, medioambientales, socioeconómicos, de limitada actividad física y una dieta inadecuada. Esta epidemia de la obesidad y sus complicaciones podría iniciarse ya en el periodo prenatal. Nuestro estudio ha valorado estos riesgos junto al efecto de los patrones del consumo alimentario sobre el riesgo de la adiposidad y de sus alteraciones metabólicas. El 44,8% de los niños y ...

  3. Respostas metabólicas em camundongos submetidos à dieta indutora de obesidade e alimentados com iogurtes probióticos e yacon (Smallanthus sonchifolius)

    OpenAIRE

    Pereira, Juciane de Abreu Ribeiro

    2013-01-01

    Muitas pesquisas têm sido realizadas na busca de terapias alternativas para a obesidade, a qual caracteriza-se por estado inflamatório que interfere em vários processos metabólicos, modificando perfil lipídico e glicêmico, podendo causar dislipidemias e resistência à insulina. Os alimentos com alegação de propriedades funcionais se destacam nesse contexto, a exemplo de iogurtes probióticos e raiz tuberosa de yacon (Smallanthus sonchinfolius). O presente trabalho objetivou avaliar os efeitos d...

  4. Nefrolitíase em pacientes pediátricos: investigação metabólica e anatômica

    Directory of Open Access Journals (Sweden)

    Luis Alberto Batista Peres

    2011-03-01

    Full Text Available Desordens metabólicas são frequentes em pacientes com nefrolitíase pediátrica. OBJETIVOS: Estudar as alterações metabólicas e anatômicas e a análise química dos cálculos encontrados em pacientes da nossa região. MÉTODOS: Este é um estudo retrospectivo em 158 crianças com evidência de formação recente de cálculos, destes apenas 109 concluíram a investigação metabólica. A investigação laboratorial consistiu de duas amostras de urina de 24 horas com dosagem de cálcio, ácido úrico, citrato, oxalato, sódio e creatinina, cistinúria qualitativa, pH urinário seguido de 12 horas de jejum e restrição hídrica, cultura da urina e análise química quando os cálculos foram disponíveis. As técnicas de imagem incluíram ultrassonografia do trato urinário e urografia excretora. RESULTADOS: Em 96,3% das crianças alguma causa foi detectada. A principal alteração metabólica encontrada foi a hipercalciúria (73,4%. Análise química dos cálculos mostrou oxalato de cálcio em 90,9% dos casos. Alterações anatômicas foram encontradas em 18,0% dos pacientes investigados, e a mais frequente foi a duplicação pieloureteral (28,6%. CONCLUSÕES:Hipercalciúria foi a desordem mais encontrada, a alteração anatômica mais comum foi a duplicação pieloureteral e oxalato de cálcio foi o constituinte químico mais frequente. Este trabalho serviu para o conhecimento das características dos pacientes pediátricos portadores de nefrolitíase em nossa região.

  5. El consumo regular de bebidas azucaradas incrementa el perfil lipídico-metabólico y los niveles de adiposidad en universitarios de Colombia

    OpenAIRE

    Robinson Ramírez-Vélez; Mónica L. Ojeda; M. Alejandra Tordecilla; Jhonatan C. Peña; José F. Meneses

    2016-01-01

    Objetivo: Examinar si el consumo de bebidas azucaradas se relaciona con alteraciones en el perfil lipídico-metabólico y con marcadores de adiposidad en una muestra de estudiantes universitarios de Colombia. Métodos: Estudio transversal en 280 voluntarios (73,9% varones) de 3 universidades de Colombia. El consumo de bebidas azucaradas se obtuvo mediante un cuestionario de frecuencia de ingesta en los últimos 7 días (BEVQ-15) en los dominios: bebidas azucaradas carbonatadas y refrescos de ju...

  6. Adherencia a la dieta mediterréna y su papel en la prevención del síndrome metabólico

    OpenAIRE

    Benabdellah, Kaouthar

    2016-01-01

    El síndrome metabólico (SM), también conocido como síndrome X, síndrome de Insulino Resistencia, o síndrome de Reaven, se define por la presencia en un individuo de un conjunto de factores tales como obesidad, hipertensión, intolerancia a la glucosa, dislipemias aterogénicas y estado protrombótico y proinflamatorio íntimamente relacionados entre sí, los cuales inducen un mayor riesgo de padecer enfermedad cardiovascular y diabetes mellitus de tipo 2, en un contexto de resistencia insulínica. ...

  7. Índice de adiposidad corporal como predictor de obesidad y de síndrome metabólico en adultos de Bogotá, Colombia

    OpenAIRE

    González-Ruíz, Katherine; Ramírez-Vélez, Robinson; Correa Bautista, Jorge Enrique

    2015-01-01

    Recientemente, Bergman et al. desarrollaron el índice de adiposidad corporal (IAC), como un marcador de obesidad por exceso de grasa corporal en la práctica clínica. En este estudio se evaluó la validez del IAC como marcador de obesidad por exceso de adiposidad, además de examinar la capacidad predictiva del IAC con componentes e índices metabólicos asociados al SM en adultos de Bogotá, Colombia. Convocatoria FODEIN-USTA 2014 Nº 2013004

  8. Estudio del efecto de los ácidos grasos poliinsaturados, polifenoles e iminociclitoles sobre marcadores relacionados con el síndrome metabólico

    OpenAIRE

    Molinar Toribio, Eunice María

    2015-01-01

    La prevalencia del denominado Síndrome Metabólico (SM) está aumentando rápidamente en todo el mundo a tal punto que se está convirtiendo en un problema de salud global. Se conoce como SM a un conjunto de factores de riesgo para la salud, que incluyen la obesidad (obesidad central), resistencia a la insulina (RI), dislipidemia, hígado graso no alcohólico y la hipertensión. Estas alteraciones se han relacionado con un alto riesgo de desarrollar diabetes tipo 2 (DM2) y enfermedades cardiovascula...

  9. Estudio del efecto de los ácidos grasos poliinsaturados, polifenoles e iminociclitoles sobre marcadores relacionados con el síndrome metabólico

    OpenAIRE

    Molinar Toribio, Eunice María

    2015-01-01

    [spa] La prevalencia del denominado Síndrome Metabólico (SM) está aumentando rápidamente en todo el mundo a tal punto que se está convirtiendo en un problema de salud global. Se conoce como SM a un conjunto de factores de riesgo para la salud, que incluyen la obesidad (obesidad central), resistencia a la insulina (RI), dislipidemia, hígado graso no alcohólico y la hipertensión. Estas alteraciones se han relacionado con un alto riesgo de desarrollar diabetes tipo 2 (DM2) y enfermedades cardiov...

  10. Síndrome metabólico y riesgo cardiovascular en pacientes con diagnóstico de esquizofrenia, trastorno esquizoafectivo y trastorno bipolar

    OpenAIRE

    Muñoz-Calero Franco, Paloma; Sánchez Sánchez, Blanca; Rodríguez Criado, Natalia; Pinilla Santos, Berta; Bravo Herrero, Sandra; Cruz Fourcade, José Fernando; Martín Aragón, Rubén

    2015-01-01

    Introducción: los pacientes con patologías mentales graves como la esquizofrenia, el trastorno esquizoafectivo y el trastorno bipolar fallecen de media 20 años antes que la población general. La muerte por problemas cardiovasculares es la primera causa de fallecimiento, a pesar de la introducción de estrategias para el control de dichos factores de riesgo. Objetivos: analizar el porcentaje de pacientes con índice de masa corporal elevado, síndrome metabólico y riesgo cardiovascular diagnostic...

  11. Dificultades en la clasificación del síndrome metabólico: El ejemplo de los adolescentes en México

    OpenAIRE

    Eneida Camarillo-Romero

    2010-01-01

    OBJETIVO. Determinar la diferencia entre las definiciones del National Cholesterol Education Program Adult Treatment Panel (ATPIII) y de la International Diabetes Federation (IDF) para síndrome metabólico (SM) en adolescentes mexicanos. MATERIAL Y MÉTODOS. Estudio transversal en 575 adolescentes de 14 a 16 años. Se utilizaron pruebas t de Student, ji cuadrada y correlación de Spearman. RESULTADOS. La prevalencia de SM fue mayor por ATPIII (18.6%) versus IDF (8.2%) (p

  12. Potenciais interações entre medicamentos usados na síndrome metabólica = Potential interactions between drugs used in metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Bandeira, Vanessa Adelina Casali

    2014-01-01

    Conclusões: O tratamento da síndrome metabólica é complexo por requerer a associação de vários medicamentos, o que aumenta os riscos de interações medicamentosas e de efeitos adversos. O presente estudo identificou um grande número de potenciais interações medicamentosas entre esses medicamentos, o que pode reduzir ou potencializar a eficácia dos mesmos e expor o usuário a riscos

  13. Impacto metabólico e inflamatorio de una comida rica en grasas saturadas y su relación con la obesidad abdominal.

    Science.gov (United States)

    Alayón, Alicia Norma; Rivadeneira, Ana Patricia; Herrera, Carlos; Guzmán, Heidy; Arellano, Dioneris; Echeverri, Isabella

    2018-05-01

    Introducción. La etapa posprandial se asocia con el incremento de marcadores relacionados con el riesgo cardiovascular, cuya intensidad depende del estado metabólico.Objetivo. Determinar el impacto de la ingestión de una comida rica en grasas saturadas sobre el perfil metabólico e inflamatorio y su relación con la obesidad abdominal.Materiales y métodos. Se hizo un ensayo clínico en 42 individuos (21 con obesidad abdominal). Se midieron, en sangre, la glucosa, la insulina, el perfil lipídico, la proteína C reactiva, los lipopolisacáridos y la interleucina 6, en ayunas y después de la ingestión.Resultados. Además de la obesidad, se registró la presencia de resistencia a la insulina y de niveles elevados de triacilglicéridos y proteína C reactiva en ayunas. Asimismo, se detectaron niveles posprandiales más elevados de glucosa, insulina y triacilglicéridos. La interleucina 6 disminuyó en el grupo de personas sin obesidad y los lipopolisacáridos aumentaron en ambos grupos.Conclusión. La ingestión de una comida rica en grasas saturadas produjo un mayor impacto en las variables glucémicas en el grupo con obesidad y, aunque afectó de forma similar los lípidos en ambos grupos, el incremento de triacilglicéridos fue mayor en presencia de una concentración basal elevada y promovió el aumento de lipopolisacáridos. El estado inflamatorio basal y posprandial afectó en mayor medida al grupo con obesidad. El momento posprandial reflejó el estado más frecuente de los individuos en un día normal y permitió evidenciar la capacidad de respuesta metabólica frente a la ingestión de alimentos, así como los estados tempranos de riesgo metabólico.

  14. Associação dos fatores demográficos, socioeconômicos e dietéticos com os componentes da síndrome metabólica em escolares com excesso de peso

    OpenAIRE

    Ana Elisa Madalena Rinaldi

    2009-01-01

    Introdução: O critério diagnóstico da síndrome metabólica na infância não está bem estabelecido, entretanto sua presença e dos seus componentes, já estão presentes, predominantemente, nas crianças com excesso de peso. Poucos estudos na população infantil mostram a influência do consumo alimentar na prevalência da síndrome metabólica. Objetivo: Verificar a relação dos fatores demográficos, socioeconômicos e dietéticos com os componentes da síndrome metabólica em escolares com excesso de peso p...

  15. Riesgo coronario según ecuación de Framingham en adultos con síndrome metabólico de la ciudad de Soledad, Atlántico. 2010

    Directory of Open Access Journals (Sweden)

    Edgar Navarro, MD., MSc.

    2012-05-01

    Resultados y conclusiones: 49,5% de los sujetos tenían síndrome metabólico según la International Diabetes Foundation (IDF, 41,4% de acuerdo con la American Heart Asociation (AHA y 20,2% con base en el Adult Treatment Panel III (ATP III. La prevalencia de síndrome metabólico fue mayor en mujeres (p>0,05; por edad se encontraron diferencias estadísticamente significativas (p<0,05. El promedio de porcentaje de riesgo cardiovascular según Framingham fue de 3% (DE ±: 4,72%, mayor en los hombres: 5,67 (DE ±: 5,24 frente a 1,01 (DE±: 3,04 en mujeres. En los hombres con síndrome metabólico el riesgo cardiovascular fue mayor.

  16. Progress of Organizational Behavior of Libraries Kütüphanelerin Örgütsel Davranışının Gelişimi

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    Mustafa Bayter

    2008-03-01

    Full Text Available The manner of librarian, determines organizational behavior of library. We can define as all of human behavior of organizational behavior, the expressions of individual while working inside of institution, perception, worth, the capacity of learning and base of organizational of human behavior. User focused organizational library, must heal of service quality everyday. The heal of service quality is possible in case of good organizational behavior. At this work, we try to work under organizational behavior, organizational culture, organizational culture and library, organizational behavior of libraries, alternation and progress of organizational behavior of libraries. Kütüphanede çalışanların davranışları, kütüphanenin örgütsel davranışını belirler. Örgütsel davranışı; bireyin kurum içinde çalışırken g��sterdiği davranışları, algıları, değerleri, öğrenme kapasitesini ve örgütsel bazdaki insan davranışının tümü olarak tanımlanmaktadır. Kullanıcı odaklı örgüt olan kütüphane, kullanıcı memnuniyeti için hizmet kalitesini her gün iyileştirmek zorundadır. Bu çalışmamızda konuyu; örgütsel davranış, örgütsel kültür, örgütsel davranış ve kütüphane, kütüphanelerin örgütsel davranışı, kütüphanenin örgütsel davranışının değişim ve gelişim analiz modeli başlıkları altında incelemeye çalıştık

  17. Detección precoz de trastornos endocrinológicos

    OpenAIRE

    M. Patricio Trincado, Dr.; B. Carolina Letelier, Dra.

    2010-01-01

    Se revisan los trastornos más comunes del sistema endocrino que se detectan en la consulta no especializada. Se hace énfasis en: a) Trastornos tiroideos tales como hipo o hipertiroidismo, nódulos de la tiroides y la importancia de la enfermedad de la tiroides durante el embarazo, b) la enfermedad adrenal en la hipertensión y el enfoque de la incidentaloma suprarrenal c) hiperparatiroidismo primario y la deficiencia de vitamina D d) Trastornos gonadal y la importancia de la detección precoz de...

  18. Detección precoz de trastornos endocrinológicos

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    M. Patricio Trincado, Dr.

    2010-09-01

    Full Text Available Se revisan los trastornos más comunes del sistema endocrino que se detectan en la consulta no especializada. Se hace énfasis en: a Trastornos tiroideos tales como hipo o hipertiroidismo, nódulos de la tiroides y la importancia de la enfermedad de la tiroides durante el embarazo, b la enfermedad adrenal en la hipertensión y el enfoque de la incidentaloma suprarrenal c hiperparatiroidismo primario y la deficiencia de vitamina D d Trastornos gonadal y la importancia de la detección precoz de la enfermedad hormonal, tanto en la disfunción ovárica y testicular.

  19. Efficient transfection of Xenobiotic Responsive Element-biosensor plasmid using diether lipid and phosphatidylcholine liposomes in differentiated HepaRG cells.

    Science.gov (United States)

    Demazeau, Maxime; Quesnot, Nicolas; Ripoche, Nicolas; Rauch, Claudine; Jeftić, Jelena; Morel, Fabrice; Gauffre, Fabienne; Benvegnu, Thierry; Loyer, Pascal

    2017-05-30

    In this study, we evaluated cationic liposomes prepared from diether-NH 2 and egg phosphatidylcholine (EPC) for in vitro gene delivery. The impact of the lipid composition, i.e. the EPC and Diether-NH 2 molar ratio, on in vitro transfection efficiency and cytotoxicity was investigated using the human HEK293T and hepatoma HepaRG cells known to be permissive and poorly permissive cells for liposome-mediated gene transfer, respectively. Here, we report that EPC/Diether-NH 2 -based liposomes enabled a very efficient transfection with low cytotoxicity compared to commercial transfection reagents in both HEK293T and proliferating progenitor HepaRG cells. Taking advantage of these non-toxic EPC/Diether-NH 2 -based liposomes, we developed a method to efficiently transfect differentiated hepatocyte-like HepaRG cells and a biosensor plasmid containing a Xenobiotic Responsive Element and a minimal promoter driving the transcription of the luciferase reporter gene. We demonstrated that the luciferase activity was induced by a canonical inducer of cytochrome P450 genes, the benzo[a]pyrene, and two environmental contaminants, the fluoranthene, a polycyclic aromatic hydrocarbon, and the endosulfan, an organochlorine insecticide, known to induce toxicity and genotoxicity in differentiated HepaRG cells. In conclusion, we established a new efficient lipofection-mediated gene transfer in hepatocyte-like HepaRG cells opening new perspectives in drug evaluation relying on xenobiotic inducible biosensor plasmids. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Enhancement of ginsenoside Rg(1) in Panax ginseng hairy root by overexpressing the α-L-rhamnosidase gene from Bifidobacterium breve.

    Science.gov (United States)

    Zhang, Ru; Zhang, Bian-Ling; Li, Gu-Cai; Xie, Tao; Hu, Teng; Luo, Zhi-Yong

    2015-10-01

    To improve the production of ginsenoside Rg1 in Panax ginseng. The α-L-rhamnosidase gene from Bifidobacterium breve (BbRha) was overexpressed into hairy root culture system using Agrobacterium rhizogenes A4. Ginsenoside Rg1 in hairy roots was obtained following transformation via overexpressed gene representing 2.2-fold higher than those of control lines. Several overexpression transgenic hairy root lines were obtained exhibiting markedly increased levels of the corresponding α-L-rhamnosidase enzymatic activity relative to control. Ginsenoside Rg1 levels in the transgenic lines were higher (2.2-fold) than those of control after following 30 days culturing, while ginsenoside Re contents in tested transgenic lines were found to be lower. The transgenic hairy roots harboring α-L-rhamnosidase gene improved the accumulation of ginsenoside Rg1 up to 3.6 mg g(-1) dry weight. BbRha gene selectively enhances the production of ginsenoside Rg1 in P. ginseng hairy roots.

  1. The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

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    Dan Lu

    2015-01-01

    Full Text Available Neural stem cells (NSCs transplanted is one of the hottest research to treat Alzheimer’s disease (AD, but cholinergic neurons from stem cells were also susceptible to cell death which Heat shock protein 70 (HSP70 was affirmed to reverse. Related to cognitive impairment, cholinergic nervous cells should be investigated and ginsenoside Rg1 (G-Rg1 was considered to increase them. We chose tert-butyl hydroperoxide (t-BHP damage model to study in vitro. Functional properties of our recombination plasmid pEGFP-C2-HSP70 were affirmed by SH-SY5Y cells. To opposite the transitory appearance of HSP70, NSCs used as the vectors of HSP70 gene overexpressed HSP70 for at least 7 days in vitro. After transfection for 3 days, G-Rg1 pretreatment for 4 hours, and coculture for 3 days, the expression of acetylcholinesterase (ChAT, synaptophysin, and the ratio of NeuN and GFAP were assessed by western blot; Morphological properties were detected by 3D reconstruction and immunofluorescence. ChAT was markedly improved in the groups contained G-Rg1. In coculture system, the ratio of neurons/astrocytes and the filaments of neurons were increased; apoptosis cells were decreased, compared to monotherapy (P<0.05. In conclusion, we demonstrated that, as a safe cotreatment affirmed in vitro, overexpression of HSP70 in NSCs plus G-Rg1 promoted nervous cells regeneration from chronic oxidative damage.

  2. Dissociação da resposta anti-hipertensiva e metabólica à losartana e espironolactona em ratos com síndrome metabólica experimental

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    Hussen Machado

    2012-12-01

    Full Text Available INTRODUÇÃO: O tratamento da hipertensão arterial (HA em indivíduos com síndrome metabólica (SM é um desafio, uma vez que terapias não medicamentosas são de difícil implementação e o tratamento farmacológico ideal não está totalmente estabelecido. OBJETIVO: Avaliar o bloqueio do sistema renina angiotensina aldosterona (SRAA na pressão arterial (PA, na função e na morfologia renais em modelo experimental de SM induzida por dieta hiperlipídica. MÉTODOS: Ratos Wistar receberam ração hiperlipídica a partir da quarta semana de vida, por 20 semanas. Os grupos tratados receberam Losartana ou Espironolactona a partir da oitava semana de vida. Avaliou-se semanalmente o peso corporal e a PA de cauda por pletismografia. Ao final do experimento, foram realizados testes de tolerância oral à glicose, perfil lipídico, clearance de creatinina, medida direta da PA, análise morfométrica renal. RESULTADOS: A administração de dieta hiperlipídica se associou ao desenvolvimento de SM, caracterizada por acúmulo central de gordura, hipertensão arterial, hiperglicemia e hipertrigliceridemia. Nesse modelo não foram observadas alterações da histomorfometria renal. O bloqueio do receptor AT1 da angiotensina II (Ang II preveniu o desenvolvimento da HA. O bloqueio mineralocorticoide não apresentou eficácia anti-hipertensiva, porém, associou-se à redução da gordura abdominal. CONCLUSÃO: A dissociação da resposta anti-hipertensiva aos bloqueios dos receptores da Ang II e mineralocorticoide indica a participação da Ang II na gênese da HA associada à obesidade. A redução da obesidade central com a Espironolactona sugere a presença de efeito adipogênico mineralocorticoide.

  3. Efecto de la terapia UVB de banda estrecha sobre la síntesis de vitamina D y los parámetros de síndrome metabólico en pacientes con psoriasis moderada y grave

    OpenAIRE

    Romaní de Gabriel, Jorge

    2013-01-01

    La psoriasis es una enfermedad cutánea crónica que ha sido relacionada con el síndrome metabólico. El objetivo de este trabajo fue evaluar en pacientes con psoriasis la presencia de alteraciones en parámetros antropométricos y marcadores séricos relacionados con el síndrome metabólico, y su modificación tras un tratamiento con ultravioleta B de banda estrecha. Comparamos a 50 pacientes con psoriasis con 50 controles emparejados por sus características antropométricas (edad, sexo e índice de m...

  4. Impacto de uma intervenção nutricional em pacientes adultos portadores de síndrome metabólica atendidos no Ambulatório de Nutrição do Hospital Escola/UFPel

    OpenAIRE

    Duval, Patrícia Abrantes

    2011-01-01

    A Síndrome Metabólica (SM) é um importante fator de risco independente para o desenvolvimento de Diabetes Mellitus tipo 2 e doença cardiovascular, sendo atribuído principalmente à presença de resistência à insulina. O rápido crescimento da SM tem sido atribuído às alterações do estilo de vida, principalmente, hábitos alimentares inadequados e sedentarismo. Os objetivos do estudo são avaliar o impacto de uma intervenção nutricional realizada em pacientes adultos portadores de Síndrome Metab...

  5. Efecto de la cantidad y del tipo de grasa de la dieta sobre la función reguladora del endotelio durante el estado posprnadial en pacientes con síndrome metabólico

    OpenAIRE

    Moreno Conde, Mirian

    2013-01-01

    Introducción. La etiología del síndrome metabólico (SM) es en gran medida desconocida, aunque probablemente sea la consecuencia de una compleja interacción entre factores genéticos, metabólicos y ambientales, entre los que destaca la dieta y sobre todo la calidad y la cantidad de la grasa. Hipótesis de trabajo: La ingesta de una dieta con alto contenido en ácidos grasos monoinsaturados (HMUFA) podría mejorar la función reguladora del endotelio en situación postprandial en pa...

  6. Papel de la testosterona y el cortisol en el síndrome metabólico y la diabetes mellitus tipo 2 Role of testosterone and cortisol in metabolic syndrome and type 2 diabetes mellitus

    OpenAIRE

    Aimée M. Álvarez Álvarez; Roberto M. González Suárez; Miguel A. Marrero Falcón

    2010-01-01

    INTRODUCCIÓN: el síndrome metabólico y la diabetes mellitus tipo 2 son trastornos metabólicos que han sido ampliamente abordados en la literatura científica por su alta incidencia, así como la alta morbilidad y mortalidad que a ellos se asocia. En los últimos años se han explorado nuevos elementos de posible impacto en su fisiopatogenia, dentro de los que se destacan los esteroides sexuales y los glucocorticoides. En este trabajo se revisaron y comentaron los conocimientos más actuales sobre ...

  7. Efeitos metabólicos da suplementação dietética com Agaricus sylvaticus em pacientes com câncer colorretal em fase pós-operatória

    OpenAIRE

    Fortes, Renata Costa

    2011-01-01

    O câncer colorretal é um dos mais comuns tipos de neoplasias malignas. Pacientes com câncer apresentam diversas anormalidades metabólicas e nos parâmetros bioquímicos e enzimáticos, com repercussões significativas sobre a qualidade de vida. Os fungos Agaricus sylvaticus têm sido utilizados como suplementos dietéticos devido às suas propriedades nutricionais, farmacológicas e organolépticas. O objetivo deste estudo foi avaliar os efeitos metabólicos da suplementação dietética com fungos Agaric...

  8. El síndrome metabólico en pacientes en tratamiento con antipsicóticos atípicos. Medidas higiénico-dietética de prevención

    OpenAIRE

    García García, Estefanía

    2014-01-01

    Los pacientes con enfermedad mental grave, en especial esquizofrenia, tienen un riesgo de padecer síndrome metabólico más elevado que el de la población general. Dicho síndrome es una entidad clínica representada por trastornos metabólicos que conforman una serie de factores de riesgo como son: obesidad abdominal, dislipemia aterogénica, hipertensión, resistencia a la insulina, y un estado protrombótico y proinflamatorio. La introducción de los antipsicóticos atípicos en los tr...

  9. Effects of diet and exercise on metabolic syndrome and the underlying factors = Efectos de la dieta y el ejercicio sobre el síndrome metabólico y sus factores

    OpenAIRE

    Morencos Martínez, Esther

    2012-01-01

    Introducción. El número de personas que padecen síndrome metabólico ha incrementado a nivel mundial durante las últimas dos décadas. Existen numerosos estudios que tratan de comparar prevalencias según los diferentes criterios y estimaciones del riesgo metabólico. De ellos se puede concluir que el principal hallazgo ha sido recalcar la necesidad de una definición estándar universal. A pesar de estas discrepancias no hay lugar a duda sobre el problema de salud pública que esto conlleva. Se nec...

  10. Gestão da Qualidade em um Órgão Público Auxiliar do Poder Legislativo Estadual

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    Napoleão Dieb Holanda Oliveira

    2011-12-01

    Full Text Available O presente artigo foi desenvolvido em um órgão da Administração Pública do Estado do Ceará, no qual fora implementado um Sistema de Gestão da Qualidade sob os requisitos da Norma ISO 9001:2000. O problema de pesquisa foi verificar quais as melhorias que o mencionado sistema proporcionou aos processos internos dessa organização. O objetivo geral foi investigar os impactos resultantes de sua implantação, e os objetivos específicos foram caracterizar o Sistema de Gestão da Qualidade – SGQ com a apresentação de algumas ferramentas de apoio à gestão, mostrar a orientação da Norma ISO 9001 para inovação e melhoria do órgão em estudo e identificar as melhorias obtidas com a adoção da estratégia. A realização da revisão da literatura e de normas específicas e a conclusão dos trabalhos contribuíram para o avanço da ciência, do estudo acadêmico e para a melhoria contínua da organização em pesquisa.

  11. Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent Tolerant Bacillus tequilensis RG-01: Thermostable and Surfactant Resistant

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    Soni Tiwari

    2014-01-01

    Full Text Available Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL in the presence of starch, peptone, and Ca2+ ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1% and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5% on amylase activity. This isolate (Bacillus tequilensis RG-01 may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

  12. Doação de órgãos: é preciso educar para avançar

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    Taise Ribeiro Morais

    Full Text Available O estudo refere-se a uma das principais dificuldades encontradas no processo de doação de órgãos: a deficiência de educação continuada específica para doação por parte da população e dos profissionais de saúde. Teve como objetivo enfatizar como a educação influi positivamente nas estatísticas de doações de órgãos. Trata-se de uma revisão bibliográfica, incluindo artigos de periódicos eletrônicos e obras literárias. Existe uma grande diferença entre o número de pessoas esperando transplante e o número de doadores. Medidas de educação contínua e políticas de saúde pública que incentivem as pessoas a manifestar o desejo de serem doadoras são estratégias importantes para amenizar esse problema.

  13. Síndrome metabólica em ambulatório cardiológico Síndrome metabólico en ambulatorio cardiológico Metabolic syndrome in outpatient cardiology clinics

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    José Bonifácio Barbosa

    2010-01-01

    Full Text Available FUNDAMENTO: No Brasil, a prevalência de síndrome metabólica (SM é pouco conhecida em várias regiões. OBJETIVO: Analisar a prevalência da SM, seus componentes e a concordância entre dois critérios diagnósticos numa população com idade > 13 anos. MÉTODOS: Estudo transversal, realizado de junho a outubro de 2007, em 719 pacientes, em ambulatórios cardiológicos de São Luís, MA. Mediu-se a pressão arterial (PA, peso, altura, circunferência abdominal e perfil lipídico. Avaliaram-se os fatores de risco para a SM segundo o critério da International Diabetes Federation (IDF. Razões de prevalência e intervalos de confiança de 95% foram estimados pela regressão de Poisson. RESULTADOS: A prevalência da SM foi maior em ambos os sexos pelo conceito da IDF (62,3% em homens e 64,6% em mulheres, em relação ao do National Cholesterol Education Program - Adult Treatment Prevention (NCEP ATPIII (48,9% em homens e 59% em mulheres. Os componentes da SM mais prevalentes foram: hipertensão arterial sistêmica HAS (87,2% e 86%; hipertrigliceridemia (84,4% e 82,5%; circunferência abdominal alterada (77,8% e 100%; HDL-c baixo (58,1% e 49,9%; e glicemia alterada (59,9% e 51,9%, pelos conceitos NCEP ATPIII e IDF, respectivamente. Após análise ajustada, idade > 60 anos e índice de massa corporal (IMC > 30 foram associados a um maior risco de SM (p FUNDAMENTO: En Brasil, la prevalencia de síndrome metabólico (SM es poco conocida en varias regiones. OBJETIVO: Analizar la prevalencia del SM, sus componentes y la concordancia entre dos criterios diagnósticos en una población con edad > 13 años. MÉTODOS: Estudio transversal, realizado de junio a octubre de 2007, en 719 pacientes, en ambulatorios cardiológicos de São Luís, MA. Se midió la presión arterial (PA, peso, altura, circunferencia abdominal y perfil lipídico. Se evaluaron los factores de riesgo para el SM según el criterio de la International Diabetes Federation (IDF. Razones

  14. Terapia nutricional nas alterações metabólicas em pessoas vivendo com HIV/aids

    Directory of Open Access Journals (Sweden)

    Marianne Falco

    2012-08-01

    Full Text Available OBJETIVO: Analisar o efeito do tratamento nutricional sobre as alterações metabólicas provocadas pelo uso da terapia antirretroviral em adultos vivendo com HIV/aids. MÉTODOS: Revisão sistemática de literatura no PubMed, Lilacs e Cochrane, entre 1996 e 2010, do tipo ensaio clínico, controlado, randomizado, crossover, adultos, vivendo com HIV/aids em uso de terapia antirretroviral e sem doenças oportunistas. A intervenção de interesse foi suplementação nutricional via oral e/ou mudança de estilo de vida por tratamento dietoterápico específico: dislipidemia, resistência insulínica, lipodistrofia e hipertensão arterial sistêmica. A escala de Jadad foi utilizada para classificação qualitativa dos artigos. RESULTADOS: Foram localizados 385 artigos e sete foram incluídos. As intervenções utilizadas nesses estudos foram: dieta, dieta mais exercício físico, dieta mais suplemento e somente suplementos. Dislipidemia foi desfecho avaliado em todos os estudos. Os estudos que avaliaram suplementação com ômega 3 encontraram redução significativa dos triglicérides. Dieta específica mais suplementação de ômega 3 mostrou aumento de HDL-colesterol. Suplementação com nicotinato de cromo não teve efeito sobre a dislipidemia. Modificação de estilo de vida, incluindo dieta e atividade física, reduziu significativamente a circunferência da cintura, lipodistrofia e pressão arterial sistólica. CONCLUSÕES: A redução de triglicérides pela suplementação com ômega 3 foi a intervenção nutricional com maiores evidências científicas. A prescrição de dieta específica parece ser a intervenção mais adequada para aumentar HDL-colesterol. Não é possível fazer inferências sobre o tratamento nutricional do colesterol total, LDL-colesterol e resistência insulínica. Modificações no estilo de vida podem promover melhora da lipodistrofia e pressão arterial.

  15. Estudo ultrastrutural de biópsias de conjuntiva em doenças metabólicas do sistema nervoso

    Directory of Open Access Journals (Sweden)

    S. Rosemberg

    1987-03-01

    Full Text Available Quarenta biópsias de conjuntivas obtidas de um número igual de crianças portadoras de doenças metabólicas do SNC, ou supostas de tal acometimento, foram analisadas do ponto de vista ultrastrutural. Em 20 casos foram observadas alterações ultrastruturais que possibilitaram firmar o diagnóstico de muco-polissacaridose em 8, gangliosidose G M1 em 6, distrofia neuro-axonal infantil em 4, gangliosidose G M1 em um e forma infantil tardia de lipofuscinose ceróide em um. Nos 20 casos em que o exame revelou-se negativo, este era esperado em dois (doença de Leigh e de Hallervorden-Spatz; em dois, o resultado revelou-se falso negativo (leucodistrofia metacromática e adrenoleucodistrofia; em 12 casos sua negatividade permitiu afastar doença de acúmulo; em 4 casos suspeitos de lipofuscinose ceróide seu resultado foi inconclusivo. O estudo ultrastrutural de biópsias de conjuntiva é um instrumento importante para o diagnóstico de doenças metabólicas do SNC.

  16. Tratamiento de la hipertensión arterial en el paciente con síndrome metabólico Hypertension treatment in patients with metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Patricio López-Jaramillo

    2010-02-01

    Full Text Available El síndrome metabólico afecta alrededor de 25% a 45% de la población colombiana de acuerdo con los criterios diagnósticos propuestos por la Federación Internacional de Diabetes, los cuales, en nuestra población, aparecen como los más útiles. Bien se sabe que el síndrome metabólico se asocia con un incremento tres a seis veces en el riesgo de desarrollar diabetes y nuevos casos de hipertensión. En Colombia, un estudio de cohorte realizado en pacientes que sufrieron infarto agudo del miocardio, demostró que la resistencia a la insulina fue el principal factor que predijo muerte cardiovascular o presencia de nuevos eventos cardio-cerebro-vasculares. Además, la presencia de síndrome metabólico se asocia con más frecuencia a daño subclínico de órgano blanco. El principal tratamiento en sujetos con síndrome metabólico es la reducción del peso corporal a través de la implementación de una dieta baja en calorías y aumento del ejercicio físico. Así, los pacientes con este síndrome necesitan la administración adicional de medicamentos antihipertensivos, antidiabéticos orales o fármacos hipolipemiantes cuando existe franca hipertensión, diabetes o dislipidemia. En vista de que el riesgo cardiovascular es alto en pacientes hipertensos con síndrome metabólico, es necesario hacer un riguroso control de la presión arterial, manteniendo los niveles siempre por debajo de 130/85 mm Hg. A no ser que existan indicaciones específicas, en los pacientes con síndrome metabólico se debe evitar el uso de beta-bloqueadores, ya que son bien conocidos sus efectos adversos en el aumento de peso, en la incidencia de nuevos casos de diabetes, en la resistencia a la insulina y en el perfil lipídico. Los diuréticos tiazídicos presentan efectos diabetogénicos y otras acciones dismetabólicas, especialmente a dosis altas, por lo que no deben utilizarse en pacientes con síndrome metabólico. Por lo tanto, los fármacos recomendados como

  17. Exposure and Tumor Fn14 Expression as Determinants of Pharmacodynamics of the Anti-TWEAK Monoclonal Antibody RG7212 in Patients with Fn14-Positive Solid Tumors

    DEFF Research Database (Denmark)

    Meulendijks, Didier; Lassen, Ulrik N; Siu, Lillian L

    2016-01-01

    PURPOSE: The TWEAK-Fn14 pathway represents a novel anticancer target that is being actively investigated. Understanding the relationship between pharmacokinetics of anti-TWEAK therapeutics and tumor pharmacodynamics is critical. We investigated exposure-response relationships of RG7212, an anti...... changes in tumor TWEAK-Fn14 signaling in paired pre- and posttreatment tumor biopsies. The objectives of the analysis were to define exposure-response relationships and the relationship between pretreatment tumor Fn14 expression and pharmacodynamic effect. Associations between changes in TWEAK-Fn14...... longer time on study was observed with high versus low RG7212 exposure. CONCLUSIONS: RG7212 reduced tumor TWEAK-Fn14 signaling in a systemic exposure-dependent manner. In addition to higher exposure, relatively high Fn14 expression might be required for pharmacodynamic effect of anti-TWEAK monoclonal...

  18. Manejo integral del paciente hipertenso con síndrome metabólico y diabetes Integrated management of hypertensive patients with metabolic syndrome and diabetes

    Directory of Open Access Journals (Sweden)

    Vicente Lahera

    2010-02-01

    Full Text Available En los últimos años se ha observado un aumento de la prevalencia de obesidad y síndrome metabólico a nivel mundial, cuyas causas habrían de buscarse fundamentalmente en el sedentarismo y la alimentación inadecuada. La obesidad y el síndrome metabólico son una primera causa para el desarrollo de enfermedad cardiovascular y diabetes mellitus tipo 2. La resistencia a la insulina desempeña un papel central en el desarrollo de las alteraciones vasculares y metabólicas que acompañan al síndrome metabólico y la obesidad: dislipidemia aterogénica, hipertensión y estado protrombótico e inflamatorio. La activación del sistema nervioso simpático y del sistema renina-angiotensina-aldosterona también participa en la mayoría de estas alteraciones. Por ello, el bloqueo de las acciones de la angiotensina II con los inhibidores de la enzima convertidora de angiotensina (IECA o los antagonistas de los receptores de angiotensina II (ARA-II, se considera una aproximación terapéutica obligatoria en estos pacientes. Es preciso considerar que muchos pacientes con múltiples factores de riesgo requieren más de un fármaco para controlar su hipertensión. La combinación de medicamentos bloqueadores de la angiotensina II y diuréticos es la más frecuente; sin embargo, en pacientes hipertensos con diabetes, síndrome metabólico o alteraciones del metabolismo de la glucosa, ésta no parece ser la más adecuada debido al potencial diabetogénico de los diuréticos tiazídicos. Por ello, la unión de IECA y calcioantagonistas se ha revelado como una combinación alternativa interesante. Los estudios ACCOMPLISH, STAR y STAR-LET, han demostrado beneficios importantes en pacientes diabéticos o con resistencia a la insulina.In the last years an increase in the prevalence of obesity and metabolic syndrome has been observed around the world. Causes for this increase are mainly related with sedentarism and inadequate diet. Obesity and metabolic syndrome

  19. Consequências metabólicas na SAOS não tratada Metabolic consequences of untreated obstructive sleep apnea syndrome

    Directory of Open Access Journals (Sweden)

    Glaucia Carneiro

    2010-06-01

    Full Text Available A associação entre SAOS e a síndrome metabólica é reconhecida, sendo denominada síndrome Z. Os critérios para a síndrome metabólica incluem pelo menos três dos seguintes fatores: obesidade central (circunferência da cintura > 102 cm em homens e > 88 cm em mulheres; triglicérides > 150 mg/dL; HDL colesterol 130/85 mmHg; e glicemia de jejum > 100 mg/dL. A obesidade central esta associada a SAOS e síndrome metabólica, havendo evidências de que a apneia do sono seja um fator de risco independente da obesidade, intolerância à glicose e resistência insulínica. Embora a obesidade central seja um fator de risco para ambas as condições, há evidências de que a apneia do sono seja um fator de risco independente para a intolerância à glicose e a resistência à insulina. Os mecanismos implicados decorrem da ativação do sistema nervoso simpático e do eixo hipotálamo-hipófise-adrenal; da ativação de fatores pró-inflamatórios, como IL-6 e TNF-α; e da diminuição dos níveis de adiponectina mediados principalmente pela hipoxemia intermitente relacionada às apneias. Apesar dessas evidências, os resultados dos estudos são controversos em relação aos benefícios do tratamento da apneia do sono com CPAP nas alterações metabólicas. Adicionalmente, os poucos estudos que abordaram a apneia do sono obstrutiva como um fator de risco para as dislipidemias apresentaram resultados discordantes. Estudos controlados, populacionais e longitudinais são necessários para esclarecer a interação entre a apneia do sono e as consequências metabólicas no sentido de se tratar adequadamente esses indivíduos.There is a recognized association between obstructive sleep apnea syndrome and metabolic syndrome, designated syndrome Z. The criteria for metabolic syndrome include at least three of the following factors: central obesity (waist circumference > 102 cm for males and > 88 cm for females; triglycerides > 150 mg/dL; HDL cholesterol 130

  20. Prevalência de síndrome metabólica em pacientes curados de câncer pediátrico

    Directory of Open Access Journals (Sweden)

    Marcelo Gil Cliquet

    2016-10-01

    Full Text Available O Câncer atinge cerca de doze mil crianças por ano no Brasil. Parte delas, quando submetida a tratamento, tende a possuir maiores chances de desenvolver Síndrome Metabólica. Introdução: As crianças e adolescentes tratadas de câncer tendem a desenvolver a síndrome metabólica devido a alterações que os radioterápicos e quimioterápicos propiciam. Essas disfunções acometem os tecidos: adiposo, osteomuscular, endotelial; o trato gastrointestinal e o fígado. Objetivos: Analisar a prevalência de síndrome metabólica em crianças curadas de câncer e comparar as variáveis estudadas. Materiais e Métodos: Avaliamos trinta e oito pacientes do Hospital GPACI que tenham terminado o tratamento de câncer há, no mínimo, cinco anos. Foram investigadas variáveis como: glicemia, pressão arterial, circunferência abdominal, HDL, triglicerídeos, tipo de câncer, tipo de tratamento e histórico familiar. Como grupo controle, utilizamos 20 irmãos dos pacientes analisados sob os mesmos parâmetros. Resultados: Sobreviventes tiveram maior chance de desenvolver síndrome metabólica (RR 2,1 Mid-p 0,27. Tanto no grupo controle quanto no grupo de curados, o HDL foi o índice que teve mais alterações, estando abaixo ou igual a 45mg/dL em 46% dos voluntários. Comparando o grupo estudado com o grupo controle, a circunferência abdominal foi o fator de maior relevância (RR 5,2 Mid-p 0,02. Discussão: Contrastamos a prevalência de síndrome metabólica encontrada nos curados de câncer tanto com seus irmãos quanto com crianças e adolescentes dos Estados Unidos da América. Em ambas comparações, a prevalência no grupo estudado foi maior, o que é semelhante a outras pesquisas realizadas. Conclusão: A circunferência abdominal foi o fator que apresentou significância estatística e maior risco de acometer os sobreviventes de câncer infantil. Diferentemente de outros estudos, os outros índices não tiveram significância estat

  1. Stable Overexpression of the Constitutive Androstane Receptor Reduces the Requirement for Culture with Dimethyl Sulfoxide for High Drug Metabolism in HepaRG Cells.

    Science.gov (United States)

    van der Mark, Vincent A; Rudi de Waart, D; Shevchenko, Valery; Elferink, Ronald P J Oude; Chamuleau, Robert A F M; Hoekstra, Ruurdtje

    2017-01-01

    Dimethylsulfoxide (DMSO) induces cellular differentiation and expression of drug metabolic enzymes in the human liver cell line HepaRG; however, DMSO also induces cell death and interferes with cellular activities. The aim of this study was to examine whether overexpression of the constitutive androstane receptor (CAR, NR1I3), the nuclear receptor controlling various drug metabolism genes, would sufficiently promote differentiation and drug metabolism in HepaRG cells, optionally without using DMSO. By stable lentiviral overexpression of CAR, HepaRG cultures were less affected by DMSO in total protein content and obtained increased resistance to acetaminophen- and amiodarone-induced cell death. Transcript levels of CAR target genes were significantly increased in HepaRG-CAR cultures without DMSO, resulting in increased activities of cytochrome P450 (P450) enzymes and bilirubin conjugation to levels equal or surpassing those of HepaRG cells cultured with DMSO. Unexpectedly, CAR overexpression also increased the activities of non-CAR target P450s, as well as albumin production. In combination with DMSO treatment, CAR overexpression further increased transcript levels and activities of CAR targets. Induction of CYP1A2 and CYP2B6 remained unchanged, whereas CYP3A4 was reduced. Moreover, the metabolism of low-clearance compounds warfarin and prednisolone was increased. In conclusion, CAR overexpression creates a more physiologically relevant environment for studies on hepatic (drug) metabolism and differentiation in HepaRG cells without the utilization of DMSO. DMSO still may be applied to accomplish higher drug metabolism, required for sensitive assays, such as low-clearance studies and identification of (rare) metabolites, whereas reduced total protein content after DMSO culture is diminished by CAR overexpression. Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics.

  2. Comparison of the removal of ethanethiol in twin-biotrickling filters inoculated with strain RG-1 and B350 mixed microorganisms.

    Science.gov (United States)

    An, Taicheng; Wan, Shungang; Li, Guiying; Sun, Lei; Guo, Bin

    2010-11-15

    This study aims to compare the biological degradation performance of ethanethiol using strain RG-1 and B350 commercial mixed microorganisms, which were inoculated and immobilized on ceramic particles in twin-biotrickling filter columns. The parameters affecting the removal efficiency, such as empty bed residence time (EBRT) and inlet concentration, were investigated in detail. When EBRT ranged from 332 to 66 s at a fixed inlet concentration of 1.05 mg L(-1), the total removal efficiencies for RG-1 and B350 both decreased from 100% to 70.90% and 47.20%, respectively. The maximum elimination capacities for RG-1 and B350 were 38.36 (removal efficiency=89.20%) and 25.82 g m(-3) h(-1) (removal efficiency=57.10%), respectively, at an EBRT of 83 s. The variation of the inlet concentration at a fixed EBRT of 110 s did not change the removal efficiencies which remained at 100% for RG-1 and B350 at concentrations of less than 1.05 and 0.64 mg L(-1), respectively. The maximum elimination capacities were 39.93 (removal efficiency=60.30%) and 30.34 g m(-3) h(-1) (removal efficiency=46.20%) for RG-1 and B350, respectively, at an inlet concentration of 2.03 mg L(-1). Sulfate was the main metabolic product of sulfur in ethanethiol. Based the results, strain RG-1 would be a better choice than strain B350 for the biodegradation of ethanethiol. Copyright © 2010 Elsevier B.V. All rights reserved.

  3. Morte encefálica, cuidados ao doador de órgãos e transplante de pulmão

    OpenAIRE

    D'Império,Fernando

    2007-01-01

    JUSTIFICATIVA E OBJETIVOS: O transplante de órgãos é aceito como uma opção para doença orgânica terminal, em pacientes bem selecionados. Este é o resultado de grandes avanços nos campos da Imunologia, da Tecnologia da Terapia Intensiva e da Farmacologia. Entretanto, os sistemas de transplantes são, atualmente, vítimas de seu próprio sucesso, à medida que as listas de espera se alongam, em contraste com a disponibilidade de órgãos, que permanece estável, acarretando crescente número de mortes ...

  4. First-in-Man Dose-Escalation Study of the Selective BRAF Inhibitor RG7256 in Patients with BRAF V600-Mutated Advanced Solid Tumors

    DEFF Research Database (Denmark)

    Dienstmann, Rodrigo; Lassen, Ulrik; Cebon, Jonathan

    2016-01-01

    V600-mutated advanced solid tumors. PATIENTS AND METHODS: Patients received RG7256 orally over 8 dose levels from 200 mg once a day (QD) to 2400 mg twice a day (BID) (50-, 100- and 150-mg tablets) using a classic 3 + 3 dose escalation design. RESULTS: In total, 45 patients were enrolled; most (87...... %) had advanced melanoma (94 % BRAF V600E). RG7256 was rapidly absorbed, with limited accumulation and dose-proportional increase in exposure up to 1950 mg BID. The maximal tolerated dose (MTD) was not reached. The most common drug-related adverse events (AEs) were dyspepsia (20 %), dry skin (18 %), rash...

  5. Recusa de doação de órgãos e tecidos para transplante relatados por familiares de potenciais doadores

    OpenAIRE

    Moraes,Edvaldo Leal de; Massarollo,Maria Cristina Komatsu Braga

    2009-01-01

    OBJETIVO: Conhecer a percepção de familiares de potenciais doadores sobre os motivos de recusa para doação de órgãos e tecidos para transplante. MÉTODOS: Trata-se de uma pesquisa qualitativa, na vertente fenomenológica, modalidade "estrutura do fenômeno situado". Participaram do estudo oito familiares que recusaram a doação dos órgãos e tecidos. RESULTADOS: Após análise das entrevistas, foram revelados dez motivos de recusa, considerados pelos familiares. CONSIDERAÇÕES FINAIS: As proposições ...

  6. Protective effects of geniposide and ginsenoside Rg1 combination treatment on rats following cerebral ischemia are mediated via microglial microRNA‑155‑5p inhibition.

    Science.gov (United States)

    Wang, Jun; Li, Dan; Hou, Jincai; Lei, Hongtao

    2018-02-01

    Geniposide, an active component of Gardenia, has been reported to protect against cerebral ischemia in animals. Ginsenoside Rg1, a component of Panax notoginseng, is usually administered in combination with Gardenia for the treatment of acute ischemic stroke; however, there are unknown effects of ginsenoside Rg1 that require further investigation. In the present study, the effects of geniposide and ginsensoide Rg1 combination treatment on focal cerebral ischemic stroke were investigated. For in vivo analysis, male rats were separated into three groups, including the (control), model and geniposide + ginsenoside Rg1 groups (n=8 per group). A middle cerebral artery occlusion model was established as the model group. The treatment group was treated with geniposide (30 mg/kg, tail vein injection) + ginsenoside Rg1 (6 mg/kg, tail vein injection), and the model group received saline instead. Neurobehavioral deficits, infarct volume, brain edema, and the expression of microRNA (miR)‑155‑5p and CD11b by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and immunohistochemistry, were assessed following 24 h of ischemia. For in vitro analysis, BV2 mouse microglial cells were cultured and exposed to geniposide (40 µg/ml) + ginsenoside Rg1 (8 µg/ml) during various durations of oxygen‑glucose deprivation (OGD). The expression levels of miR‑155‑5p, pri‑miR‑155 and pre‑miR‑155 were detected by RT‑qPCR. The results demonstrated that increases in brain infarct volume, edema volume, CD11b‑positive cells and miR‑155‑5p levels were alleviated following geniposide + ginsenoside administration in rats exposed to ischemia. Furthermore, geniposide + ginsenoside Rg1 treatment suppressed the miR‑155‑5p, pri‑miR‑155 and pre‑miR‑155 expression levels in OGD‑injured BV2 microglial cells. The results of the present study demonstrated that tail vein administration of geniposide in combination with ginsenoside Rg1

  7. Soluble epoxide hydrolase in the generation and maintenance of high blood pressure in spontaneously hypertensive rats

    NARCIS (Netherlands)

    Koeners, Maarten P.; Wesseling, Sebastiaan; Ulu, Arzu; Lopez Sepulveda, Rocio; Morisseau, Christophe; Braam, Branko; Hammock, Bruce D.; Joles, Jaap A.

    Koeners MP, Wesseling S, Ulu A, Sepulveda RL, Morisseau C, Braam B, Hammock BD, Joles JA. Soluble epoxide hydrolase in the generation and maintenance of high blood pressure in spontaneously hypertensive rats. Am J Physiol Endocrinol Metab 300: E691-E698, 2011. First published January 25, 2011; doi:

  8. Thyroid Disorders in Accra, Ghana

    African Journals Online (AJOL)

    User

    to enhance thyroid-targeted expression of sodium/iodide symporter. J Clin Endocrinol. Metab 89(5): 2344-2350. Mariotti, S, Cambuli, VM (2007) Cardiovascular risk in elderly hypothyroid patients. Thyroid 17. (11): 1067-1073. Mariotti, S, Franceschi, C, Cossarizza, A, Pinchera, A. (1995) The aging thyroid. Endocr Rev 16(6):.

  9. Effect of ghrelin on glucose regulation in mice

    NARCIS (Netherlands)

    Chacko, Shaji K.; Haymond, Morey W.; Sun, Yuxiang; Marini, Juan C.; Sauer, Pieter J. J.; Ma, Xiaojun; Sunehag, Agneta L.

    Chacko SK, Haymond MW, Sun Y, Marini JC, Sauer PJJ, Ma X, Sunehag AL. Effect of ghrelin on glucose regulation in mice. Am J Physiol Endocrinol Metab 302: E1055-E1062, 2012. First published February 14, 2011; doi:10.1152/ajpendo.00445.2011.-Improvement of glucose metabolism after bariatric surgery

  10. Oral glucose intake inhibits hypothalamic neuronal activity more effectively than glucose infusion

    NARCIS (Netherlands)

    Smeets, P.A.M.; Vidarsdottir, S.; Graaf, de C.; Stafleu, A.; Osch, M.J.P.; Viergever, M.A.; Pijl, H.; Grond, van der J.

    2007-01-01

    Oral glucose intake inhibits hypothalamic neuronal activity more effectively than glucose infusion. Am J Physiol Endocrinol Metab 293: E754-E758, 2007. First published June 12, 2007; doi:10.1152/ajpendo.00231.2007. - We previously showed that hypothalamic neuronal activity, as measured by the blood

  11. An antiinflammatory actions of exenatide

    Directory of Open Access Journals (Sweden)

    S Yu Vorotnikova

    2012-12-01

    Full Text Available Реферат по статье: Chaudhuri A, Ghanim H, Vora M, Sia CL, Korzeniewski K, Dhindsa S, Makdissi A, Dandona P. Exenatide exerts a potent antiinflammatory effect. J Clin Endocrinol Metab. 2012 Jan; 97(1: 198-207.

  12. A phase 1 study to evaluate the safety and LDL cholesterol-lowering effects of RG7652, a fully human monoclonal antibody against proprotein convertase subtilisin/kexin type 9.

    Science.gov (United States)

    Baruch, Amos; Luca, Diana; Kahn, Robert S; Cowan, Kyra J; Leabman, Maya; Budha, Nageshwar R; Chiu, Cecilia P C; Wu, Yan; Kirchhofer, Daniel; Peterson, Andrew; Davis, John C; Tingley, Whittemore G

    2017-07-01

    Proprotein convertase subtilisin/kexin type 9 (PCSK9) downregulates low-density lipoprotein (LDL) receptors, thereby leading to a rise in circulating LDL cholesterol (LDL-C). RG7652 is a fully human monoclonal antibody against PCSK9. This placebo-controlled, phase 1 ascending-dose study in healthy subjects evaluated the safety of RG7652 and its efficacy as a potential LDL-C-lowering drug. Anti-PCSK9 antibody therapy safely and effectively reduces LDL-C. Subjects (N = 80) were randomized into 10 cohorts. Six sequential single-dose cohorts received 10, 40, 150, 300, 600, or 800 mg of RG7652 via subcutaneous injection. Four multiple-dose cohorts received 40 or 150 mg of RG7652 once weekly for 4 weeks, either with or without statin therapy (atorvastatin). Adverse events (AEs) were generally mild; the most common AEs were temporary injection-site reactions. No serious AEs, severe AEs, AEs leading to study-drug discontinuation, or dose-limiting toxicities were reported. RG7652 monotherapy reduced mean LDL-C levels by up to 64% and as much as 100 mg/dL at week 2; the effect magnitude and duration increased with dose (≥57 days following a single RG7652 dose ≥300 mg). Exploratory analyses showed reduced oxidized LDL, lipoprotein(a), and lipoprotein-associated phospholipase A2 with RG7652. Antidrug antibody against RG7652 tested positive in 2 of 60 (3.3%) RG7652-treated and in 4 of 20 (20.0%) placebo-treated subjects. Simultaneous atorvastatin administration did not appear to impact the pharmacokinetic profile or lipid-lowering effects of RG7652. Overall, RG7652 elicited substantial and sustained dose-related LDL-C reductions with an acceptable safety profile and minimal immunogenicity. © 2017 Wiley Periodicals, Inc.

  13. Morfologia e topografia dos órgãos genitais masculinos externos da paca (Cuniculus paca Linnaeus, 1766

    Directory of Open Access Journals (Sweden)

    Edson Moreira Borges

    2013-07-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2013v26n4p209 A paca (Cuniculus paca é uma espécie de roedor da família Cuniculidae, encontrada na América do Sul, desde a Bacia do Rio Orinoco até o Paraguai. Apesar de amplamente estudada quanto aos aspectos morfológico e reprodutivo, ainda não há registros sobre a morfologia do aparelho reprodutor masculino dessa espécie. Assim, objetivou-se avaliar não só a anatomia macro e microscópica dos órgãos genitais externos dessa espécie, mas, também, sua topografia. Foram estudados os órgãos genitais externos de 10 pacas, machos, adultas; 5 espécimes, fixados em solução aquosa de formol a 10%, foram estudados macroscopicamente e de 5 espécimes foram coletados fragmentos do aparelho reprodutor, para análise microscópica. Os testículos apresentavam parênquima estruturado em túbulos seminíferos e o epitélio germinativo repousava sobre sua membrana basal. Esses órgãos estavam localizados na cavidade abdominal, no trajeto inguinal ou no interior do escroto. O pênis, fibroelástico, localizava-se na região púbica, em direção caudal. Sua glande, revestida por epitélio queratinizado, era recoberta pelo prepúcio e, abaixo deste, identificou-se uma estrutura delgada cartilagínea com bordas serreadas. Um par de esporões ósseos abrigava-se em um saco ventral à uretra, cuja mucosa era revestida por epitélio de transição. O ducto epididimário apresentava-se enovelado na cabeça, continha corpo e cauda, sendo a cabeça revestida por epitélio pseudoestratificado estereociliado, e a cauda por epitélio cúbico simples; desta originava-se o ducto deferente, revestido por epitélio estratificado colunar.

  14. Ação antagônica e de metabólitos bioativos de Trichoderma spp. contra os patógenos Sclerotium rolfsii e Verticillium dahliae

    Directory of Open Access Journals (Sweden)

    Carolina Oliveira Isaias

    2014-03-01

    Full Text Available O efeito antagônico exercido por fungos do gênero Trichoderma foi avaliado contra os fitopatógenos Sclerotium rolfsii e Verticillium dahliae, isolados de áster ornamental e morangueiro, respectivamente. Utilizaram-se 20 isolados do antagonista, pertencentes às espécies T. asperellum, T. hamatum, T. harzianum, T. koningiopsis, T. spirale e Trichoderma sp. O antagonismo foi verificado por meio de testes de culturas pareadas e produção de metabólitos bioativos pelo método de sobreposição de placas. A maioria dos isolados exibiu efeito inibitório sobre o crescimento micelial de S. rolfsii e V. dahliae quando comparado com as testemunhas. Excetuando-se os testes de produção de metabólitos não-voláteis, verificou-se maior ação inibitória contra V. dahliae. Mesmo os metabólitos não-voláteis termoestáveis apresentaram efeito inibitório contra ambos os patógenos testados, mostrando que os metabólitos secundários do antagonista exercem efeito significativo sobre essas espécies patogênicas.

  15. The human hepatocyte cell lines IHH and HepaRG: models to study glucose, lipid and lipoprotein metabolism.

    Science.gov (United States)

    Samanez, Carolina Huaman; Caron, Sandrine; Briand, Olivier; Dehondt, Hélène; Duplan, Isabelle; Kuipers, Folkert; Hennuyer, Nathalie; Clavey, Véronique; Staels, Bart

    2012-07-01

    Metabolic diseases reach epidemic proportions. A better knowledge of the associated alterations in the metabolic pathways in the liver is necessary. These studies need in vitro human cell models. Several human hepatoma models are used, but the response of many metabolic pathways to physiological stimuli is often lost. Here, we characterize two human hepatocyte cell lines, IHH and HepaRG, by analysing the expression and regulation of genes involved in glucose and lipid metabolism. Our results show that the glycolysis pathway is activated by glucose and insulin in both lines. Gluconeogenesis gene expression is induced by forskolin in IHH cells and inhibited by insulin in both cell lines. The lipogenic pathway is regulated by insulin in IHH cells. Finally, both cell lines secrete apolipoprotein B-containing lipoproteins, an effect promoted by increasing glucose concentrations. These two human cell lines are thus interesting models to study the regulation of glucose and lipid metabolism.

  16. Comparación de dos metodologías utilizadas para la determinación del síndrome metabólico en población adulta

    Directory of Open Access Journals (Sweden)

    Yiby Forero

    2013-06-01

    Full Text Available Introducción. El síndrome metabólico se caracteriza por la aparición simultánea o secuencial de diversas alteraciones metabólicas e inflamatorias a nivel molecular, celular o hemodinámico, asociadas a resistencia a la insulina y adiposidad de predominio visceral. Diferentes grupos de expertos, como la International Diabetes Federation (IDF y el National Cholesterol Education Program (NCEP, hantratado de elaborar criterios para su diagnóstico. Objetivo. Establecer los niveles de concordancia de los criterios de diagnóstico para el síndrome metabólico. Material y métodos. El estudio incluyó 357 individuos. Se hizo el análisis descriptivo de variables cualitativas y de resultados de laboratorio y medición antropométrica para la determinación de la prevalencia de síndrome metabólico, según las definiciones de los dos grupos mencionados. Se utilizaron concordancia kappa y regresión logística, para la comparación de las metodologías. Resultados. Las prevalencia del síndrome metabólico, según la International Diabetes Federation, es de 19,9 % y por el Adult Treatment Panel (ATP III fue de 10,4 %. La estimación de concordancia entre los criterios diagnósticos de la Federación y los del Panel fue escasa (fair agreement (κ=0,3997;IC95%: 0,28-0,52. Conclusiones. En la población trabajadora que participó en este estudio, la prevalencia del síndrome metabólico se presentó en mayor porcentaje utilizando la definición de la International Diabetes Federation y fue más alta en los hombres que en las mujeres. Los criterios diagnósticos para el síndrome metabólico deben usarse teniendo en cuenta la población en estudio, así como el sexo y la edad.   http://dx.doi.org/10.7705/biomedica.v33i2.704

  17. Soft SUSY breaking parameters and RG running of squark and slepton masses in large volume Swiss Cheese compactifications

    International Nuclear Information System (INIS)

    Misra, Aalok; Shukla, Pramod

    2010-01-01

    We consider type IIB large volume compactifications involving orientifolds of the Swiss Cheese Calabi-Yau WCP 4 [1,1,1,6,9] with a single mobile space-time filling D3-brane and stacks of D7-branes wrapping the 'big' divisor Σ B (as opposed to the 'small' divisor usually done in the literature thus far) as well as supporting D7-brane fluxes. After reviewing our proposal of (Misra and Shukla, 2010) for resolving a long-standing tension between large volume cosmology and phenomenology pertaining to obtaining a 10 12 GeV gravitino in the inflationary era and a TeV gravitino in the present era, and summarizing our results of (Misra and Shukla, 2010) on soft supersymmetry breaking terms and open-string moduli masses, we discuss the one-loop RG running of the squark and slepton masses in mSUGRA-like models (using the running of the gaugino masses) to the EW scale in the large volume limit. Phenomenological constraints and some of the calculated soft SUSY parameters identify the D7-brane Wilson line moduli as the first two generations/families of squarks and sleptons and the D3-brane (restricted to the big divisor) position moduli as the two Higgses for MSSM-like models at TeV scale. We also discuss how the obtained open-string/matter moduli make it easier to impose FCNC constraints, as well as RG flow of off-diagonal squark mass(-squared) matrix elements.

  18. Soft SUSY breaking parameters and RG running of squark and slepton masses in large volume Swiss Cheese compactifications

    Science.gov (United States)

    Misra, Aalok; Shukla, Pramod

    2010-03-01

    We consider type IIB large volume compactifications involving orientifolds of the Swiss Cheese Calabi-Yau WCP[1,1,1,6,9] with a single mobile space-time filling D3-brane and stacks of D7-branes wrapping the “big” divisor ΣB (as opposed to the “small” divisor usually done in the literature thus far) as well as supporting D7-brane fluxes. After reviewing our proposal of [1] (Misra and Shukla, 2010) for resolving a long-standing tension between large volume cosmology and phenomenology pertaining to obtaining a 10 GeV gravitino in the inflationary era and a TeV gravitino in the present era, and summarizing our results of [1] (Misra and Shukla, 2010) on soft supersymmetry breaking terms and open-string moduli masses, we discuss the one-loop RG running of the squark and slepton masses in mSUGRA-like models (using the running of the gaugino masses) to the EW scale in the large volume limit. Phenomenological constraints and some of the calculated soft SUSY parameters identify the D7-brane Wilson line moduli as the first two generations/families of squarks and sleptons and the D3-brane (restricted to the big divisor) position moduli as the two Higgses for MSSM-like models at TeV scale. We also discuss how the obtained open-string/matter moduli make it easier to impose FCNC constraints, as well as RG flow of off-diagonal squark mass(-squared) matrix elements.

  19. Tailored liquid chromatography-mass spectrometry analysis improves the coverage of the intracellular metabolome of HepaRG cells.

    Science.gov (United States)

    Cuykx, Matthias; Negreira, Noelia; Beirnaert, Charlie; Van den Eede, Nele; Rodrigues, Robim; Vanhaecke, Tamara; Laukens, Kris; Covaci, Adrian

    2017-03-03

    Metabolomics protocols are often combined with Liquid Chromatography-Mass Spectrometry (LC-MS) using mostly reversed phase chromatography coupled to accurate mass spectrometry, e.g. quadrupole time-of-flight (QTOF) mass spectrometers to measure as many metabolites as possible. In this study, we optimised the LC-MS separation of cell extracts after fractionation in polar and non-polar fractions. Both phases were analysed separately in a tailored approach in four different runs (two for the non-polar and two for the polar-fraction), each of them specifically adapted to improve the separation of the metabolites present in the extract. This approach improves the coverage of a broad range of the metabolome of the HepaRG cells and the separation of intra-class metabolites. The non-polar fraction was analysed using a C18-column with end-capping, mobile phase compositions were specifically adapted for each ionisation mode using different co-solvents and buffers. The polar extracts were analysed with a mixed mode Hydrophilic Interaction Liquid Chromatography (HILIC) system. Acidic metabolites from glycolysis and the Krebs cycle, together with phosphorylated compounds, were best detected with a method using ion pairing (IP) with tributylamine and separation on a phenyl-hexyl column. Accurate mass detection was performed with the QTOF in MS-mode only using an extended dynamic range to improve the quality of the dataset. Parameters with the greatest impact on the detection were the balance between mass accuracy and linear range, the fragmentor voltage, the capillary voltage, the nozzle voltage, and the nebuliser pressure. By using a tailored approach for the intracellular HepaRG metabolome, consisting of three different LC techniques, over 2200 metabolites can be measured with a high precision and acceptable linear range. The developed method is suited for qualitative untargeted LC-MS metabolomics studies. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Obesidade e síndrome metabólica na infância e adolescência Obesity and metabolic syndrome in infancy and adolescence

    Directory of Open Access Journals (Sweden)

    Cecília Lacroix de Oliveira

    2004-06-01

    Full Text Available Nas últimas décadas a prevalência da obesidade vem apresentando um aumento em vários países ao redor do mundo. Este fato é preocupante, já que o excesso de gordura corporal, principalmente a abdominal, está diretamente relacionado com alterações do perfil lipídico, com o aumento da pressão arterial e a hiperinsulinemia, considerados fatores de risco para o desenvolvimento de doenças crônicas, como o diabetes melito tipo 2 e as doenças cardiovasculares. Níveis elevados de leptina e de ácido úrico e a alteração dos fatores fibrinolíticos também têm sido observados em indivíduos obesos. O conjunto destas alterações tem sido descrito como "síndrome metabólica" ou "síndrome da resistência à insulina", já que a hiperinsulinemia tem um papel importante no desenvolvimento dos outros componentes da síndrome metabólica. Entretanto, questiona-se se estas alterações já estão presentes em crianças e adolescentes obesos. Este artigo descreve a fisiopatologia dos componentes da síndrome metabólica e esclarece como este processo ocorre na faixa etária mais jovem.The prevalence of obesity is increasing all over the world in the last decades. This is a concerning fact since the excess of body fat, especially abdominal fat, is directly related to the lipidic profile that is altered, to the increase of arterial pressure and hyperinsulinemia. All of these are considered risk factors to the onset of chronic-degenerative diseases such as type 2 diabetes and cardiovascular disease. High levels of leptin and the alterations of fibrinolytic factors have also been observed in obese individuals. These modifications are described as "metabolic syndrome" or "insulin resistance syndrome", since hyperinsulinemia has an important role in the development of other components of the metabolic syndrome. However, the question is if these alterations are already present in these children and adolescents. This paper describes the

  1. El síndrome metabólico y su prevalencia en la población que acude a una farmacia comunitaria de Sevilla

    Directory of Open Access Journals (Sweden)

    Guerra Hidalgo A

    2011-12-01

    Full Text Available INTRODUCCIÓN Y OBJETIVOS La prevalencia del síndrome metabólico y su repercusión cardiovascular requiere de medidas dirigidas a su prevención y control. El farmacéutico comunitario, como sanitario más próximo a la población, juega un papel fundamental en el manejo de uno de los factores más determinantes en su desarrollo: el estilo de vida. El objetivo es estimar la prevalencia del síndrome metabólico en la población que acude a las farmacias de Sevilla e identificar sus factores asociados. MÉTODOS Estudio transversal con 69 pacientes reclutados desde enero a agosto de 2008. La presencia de síndrome metabólico se determinó usando los criterios de la Federación Internacional de Diabetes. RESULTADOS La prevalencia hallada es del 33,3%. El componente más frecuente es la obesidad (53,6%, seguida por hipertensión (44,9%, hiperglucemia (40,6%, hipertrigliceridemia (34,8% e hipocolesterolemia HDL (24,6%. El sedentarismo (p=0,002, el elevado consumo de café (p=0,036 y carnes rojas y embutidos (p=0,004, unida a la baja ingesta de pescado (p<0,001, frutas y verduras (p<0,001 y productos lácteos (p=0,027 se han identificado como factores de riesgo para su desarrollo. CONCLUSIONES La prevalencia hallada es de las más altas encontradas en España. Es necesario incidir en la adopción de hábitos de vida saludable que disminuyan su repercusión sanitaria. El farmacéutico comunitario debe aprovechar su posición estratégica para educar a la población de riesgo.

  2. Rakverre Pauluse kirik - Kalevi hall - Pärdi muusikamaja = St. Paul's Church - Kalev Hall - Pärt Music House / Raul Järg

    Index Scriptorium Estoniae

    Järg, Raul, 1973-

    2009-01-01

    Rakvere linna peaarhitekt, žürii liige Raul Järg rahvusvahelisest arhitektuurivõistlusest, mille ülesandeks oli pakkuda lahendus Rakvere Pauluse kiriku kasutuselevõtmiseks Arvo Pärdi muusikamajana ja kirikuesise väljaku ümberkujundamiseks, auhinnatud töödest. 1. preemia said Kristiina Aasvee, Kristiina Hussar ja Anne Kose (Celander Projekt)

  3. Situation analysis of physical independence of the equipment and safety circuits of Almaraz NPP regarding R.G. 1.75 rev.3 (2005)

    International Nuclear Information System (INIS)

    Seijas Portela, S.

    2010-01-01

    Situation analysis of physical independence of the electrical equipment and circuits CN safety Almaraz about R.G. 1.75 rev. 3. (2005) The aim of this paper is to present the work done in the analysis of the physical separation of redundant safety electrical equipment (emergency diesel generators, medium voltage, electrical cabinets, etc.) and physical separation of circuits and electrical conduits.

  4. Considerações gerais sôbre os órgãos odoríferos sexuais dos machos dos Lepidópteros

    Directory of Open Access Journals (Sweden)

    Rudolf Barth

    1953-12-01

    Full Text Available São determinadas as seguintes regras sôbre a função dos órgãos odoríferos dos machos dos Lepidópteros: l.ª regra: A secreção das glândulas age em forma de gás sobre a fêmea diminuindo a reação normal de fuga e provocando a disposição e preparação para a copulação. 2.ª regra: Os órgãos odoríferos nos machos de um grupo de espécies parentes encontram-se constantemente nos mesmos órgãos possuindo o mesmo tipo de estrutura (constância orgânica . 3.ª regra: As partes quitinosas (escamas odoríferas, cerdas distribuidoras, inserções etc. são especificadas para cada espécie (constância de forma. 4.ª regra: O grau de desenvolvimento e com isto o grau de eficiência dos órgãos odoríferos dos Lepidópteros noturnos é sempre mais elevado do que os dos diurnos.

  5. A phase I monotherapy study of RG7212, a first-in-class monoclonal antibody targeting TWEAK signaling in patients with advanced cancers

    DEFF Research Database (Denmark)

    Lassen, Ulrik N; Meulendijks, Didier; Siu, Lilian L

    2015-01-01

    activation. A phase I study of RG7212, a humanized anti-TWEAK IgG1κ monoclonal antibody, was conducted in patients with advanced solid tumors expressing Fn14. EXPERIMENTAL DESIGN: Dose escalations, over a 200- to 7,200-mg range, were performed with patients enrolled in weekly (QW), bi-weekly (Q2W), or every...

  6. A reversion of an IL2RG mutation in combined immunodeficiency providing competitive advantage to the majority of CD8+ T cells

    NARCIS (Netherlands)

    Kuijpers, Taco W.; van Leeuwen, Ester M. M.; Barendregt, Barbara H.; Klarenbeek, Paul; Aan de Kerk, Daan J.; Baars, Paul A.; Jansen, Machiel H.; de Vries, Niek; van Lier, René A. W.; van der Burg, Mirjam

    2013-01-01

    Mutations in the common gamma chain (γc, CD132, encoded by the IL2RG gene) can lead to B(+)T(-)NK(-) X-linked severe combined immunodeficiency, as a consequence of unresponsiveness to γc-cytokines such as interleukins-2, -7 and -15. Hypomorphic mutations in CD132 may cause combined

  7. Liver Progenitor Cell Line HepaRG Differentiated in a Bioartificial Liver Effectively Supplies Liver Support to Rats with Acute Liver Failure

    NARCIS (Netherlands)

    Nibourg, Geert A. A.; Chamuleau, Robert A. F. M.; van der Hoeven, Tessa V.; Maas, Martinus A. W.; Ruiter, An F. C.; Lamers, Wouter H.; Oude Elferink, Ronald P. J.; van Gulik, Thomas M.; Hoekstra, Ruurdtje

    2012-01-01

    A major roadblock to the application of bioartificial livers is the need for a human liver cell line that displays a high and broad level of hepatic functionality. The human bipotent liver progenitor cell line HepaRG is a promising candidate in this respect, for its potential to differentiate into

  8. Revisão integrativa: indicadores de resultado processo de doação de órgãos e transplantes

    Directory of Open Access Journals (Sweden)

    Agenor Spallini Ferraz

    2013-09-01

    Full Text Available OBJETIVO: Identificar por meio da revisão integrativa os principais indicadores de resultado do processo de doação de órgãos e transplantes utilizados e transplantes no Brasil e no mundo. A revisão integrativa da literatura foi realizada nas bases de dados bibliográficas MedLine/PubMed e LILACS e sites governamentais e não governamentais no período de 1995 e 2011. Os descritores/palavras chaves utilizados foram doação de órgãos, transplante de órgãos e resultados em saúde, sendo selecionados 26 artigos e nove sites. A classificação do nível de evidência dos artigos variou de um a seis. RESULTADOS: Nível de evidência dos artigos na sua maioria 66,6% foi quatro (12:18 PubMed todos os artigos da base LILACS (8. Os indicadores apresentados nos artigos se propõem a avaliar, mensurar e controlar dados relacionados ao perfil do doador, condições clínicas e hospitalares, tempo de isquemia e tamanho do órgão, procedimento cirúrgico e as complicações advindas do transplante.

  9. Preventive effects of ginsenoside Rg1 on post-traumatic stress disorder (PTSD)-like behavior in male C57/B6 mice.

    Science.gov (United States)

    Wang, Zhongli; Zhu, Kexuan; Chen, Lin; Ou Yang, Liufeng; Huang, Yufang; Zhao, Yunan

    2015-09-25

    We investigated the preventive effects of Rg1 on a model of mouse post-traumatic stress disorder (PTSD) induced by electric shock combined with situation reminder and explored the underlying mechanism. In the experiment, before the PTSD animal model was developed, Rg1 (10, 5, and 2.5mg/kg) was orally administered for one week. After the animal model was established, PTSD-like behavior was observed using elevated plus maze, black and light box, and open field tests. One hour after the behavior test, all mice were sacrificed, and then serum corticosterone (CORT) and hypothalamus corticotrophin-releasing hormone (CRH) assays were performed. Results showed that Rg1 (5mg/kg) treatments relieved PTSD-like behavior by altering elevated serum corticosterone and hypothalamus CRH levels. By contrast, fluoxetine (3mg/kg) treatment reversed the behavior changes and had no effect on increased CORT and CRH levels. These findings confirmed the preventive effect of Rg1 in PTSD model. Decreasing CORT and CRH levels may be one of the underlying mechanisms. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  10. QTL list: SpRg-7 [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available entage concerning shoot regeneration 2 ME10-141 ... Chr07 19.51 6.84 ... 10.1186/1471-2229-11-140 22014149 ... QT73653 Solanum lycopersicum Solanaceae SpRg-7 In vitro plant regeneraion bud perc

  11. Predisposition to metabolic acidosis induced by topiramate Predisposição a acidose metabólica induzida por topiramato

    Directory of Open Access Journals (Sweden)

    MARIA AUGUSTA MONTENEGRO

    2000-12-01

    Full Text Available RATIONALE: Metabolic acidosis induced by topiramate is a well documented but infrequent adverse event. The objective was to demonstrate the lowering of carbon dioxide serum levels, which is usually asymptomatic but may facilitate the occurrence of metabolic acidosis in patients using topiramate. METHODS: We evaluated, prospectively, the carbon dioxide serum levels of 18 patients seen at the epilepsy clinic of our university hospital, before and 3 months after introducing topiramate. RESULTS: Five patients were female and 13 were male, age ranging from 2 to 16 years old (mean=9.3. Carbon dioxide mean serum levels were 25 and 21.2 mmol/L (normal = 22 to 30, before and 3 months after introducing topiramate, respectively. Dose ranged from 2.08 to 11.76 mg/kg/day (mean=6.7mg/kg/day. Adverse events were anorexia, nausea and somnolence. CONCLUSION: We conclude that the lowering of carbon dioxide serum levels induced by topiramate is mostly asymptomatic, but may facilitate the occurrence of metabolic acidosis. Since patients in use of topiramate have refractory epilepsy, they may need epilepsy surgery, and must be carefully monitored for the risk of metabolic acidosis during surgery.INTRODUÇÃO: Acidose metabólica induzida por topiramato é evento adverso pouco frequente, mas bem documentado. Nosso objetivo foi demonstrar a diminuição dos níveis de dióxido de carbono, muitas vezes assintomática, mas que pode predispor ao aparecimento de acidose metabólica. MATERIAL E MÉTODO: Avaliamos prospectivamente os níveis de dióxido de carbono de 18 pacientes acompanhados no ambulatório de epilepsia infantil da UNICAMP, antes e 3 meses após o início do uso de topiramato. RESULTADOS: Foram avaliados 18 pacientes com idade entre 2 e 16 anos (média = 9,3 anos. Cinco pacientes eram do sexo feminino e 13, do sexo masculino. Os níveis médios de dióxido de carbono antes e após o uso de topiramato foram 25 e 21,2 mmol/L (normal = 22 a 30

  12. Espectrometria de massas avançada em estudos estruturais de metabólitos de fármacos e proteínas

    OpenAIRE

    Alexandre Ferreira Gomes

    2014-01-01

    Resumo: Este trabalho foi dividido em dois capítulos, tendo como temática geral a aplicação de técnicas modernas baseadas em espectrometria de massas (MS) no estudo da biotransformação de fármacos (capítulo I) e em proteômica estrutural por ligação cruzada e mobilidade iônica (capítulo II). No capítulo I, foram estudadas rotas de fragmentação por dissociação induzida por colisão (CID), perfis de metabólitos in vivo em ratos e farmacocinética de derivados de 4-anilinoquinazolina, candidatos a ...

  13. Fatores dietéticos na prevenção e tratamento de comorbidades associadas à síndrome metabólica

    OpenAIRE

    Santos,Cláudia Roberta Bocca; Portella,Emilson Souza; Avila,Sonia Silva; Soares,Eliane de Abreu

    2006-01-01

    A síndrome metabólica constitui um fator de risco cardiovascular, sendo caracterizada pela associação de hipertensão arterial sistêmica, obesidade abdominal, tolerância à glicose prejudicada, hipertrigliceridemia e baixas concentrações sangüíneas de HDL-colesterol, além dos estados pró-trombótico e pró-inflamatório observados. No entanto, a alimentação adequada, associada a outras modificações no estilo de vida, tais como prática regular de atividade física e abandono do tabagismo, contribui ...

  14. Intervenções nutricionais em Síndrome Metabólica: uma revisão sistemática

    OpenAIRE

    Leão, Leila Sicupira Carneiro de Souza; Moraes, Milena Miranda de; Carvalho, Giulia Xavier de; Koifman, Rosalina Jorge

    2011-01-01

    Não existe consenso sobre a estratégia nutricional mais adequada para tratar a Síndrome Metabólica (SM), de tal forma que ocorra redução do risco cardiovascular. O presente estudo foi desenhado para avaliar a força de evidência dos benefícios de diferentes intervenções nutricionais na remissão da SM. A busca virtual foi realizada nas bases de dados Medline, Cochrane Library e PubMed, de ensaios clínicos randomizados publicados no período entre 1999 a 2009, em qualquer língua, em indivíduos co...

  15. Nutritional support in children under metabolic stress Soporte nutricional en el niño con estrés metabólico

    Directory of Open Access Journals (Sweden)

    María Elsy Sepúlveda Hincapié

    1999-04-01

    Full Text Available A review is presented on metabolic changes that occur in children under stress, and on the organs that are affected by the response to it; also considered are the different resources that are presently available in order to maintain nutrition in these children and to improve their probabilities of recovery. Se hace una revisión acerca de los cambios metabólicos que ocurren en el niño durante el estrés y los órganos que se afectan en su respuesta; se tienen en cuenta además, los diferentes recursos que actualmente se pueden utilizar con el fin de mantener la nutrición del niño durante este tipo de situación, mejorando sus posibilidades de recuperación.

  16. Efeitos adversos metabólicos de antipsicóticos e estabilizadores de humor Metabolic side effects of antipsychotics and mood stabilizers

    Directory of Open Access Journals (Sweden)

    Paulo José Ribeiro Teixeira

    2006-08-01

    Full Text Available INTRODUÇÃO: Um aumento na incidência de obesidade e diabetes melito entre pacientes psiquiátricos foi observado ainda na década de 60, como resultado indesejável do uso de antipsicóticos. Nos anos 80 e 90, a reabilitação da clozapina, a síntese dos demais antipsicóticos atípicos e a disseminação do uso do lítio e do ácido valpróico chamaram novamente a atenção para os efeitos metabólicos desses medicamentos. Este estudo tem por objetivo revisar a literatura médica a respeito dos efeitos adversos metabólicos associados ao uso de antipsicóticos e estabilizadores de humor. MÉTODO: Foi realizada uma extensa pesquisa nas bases de dados MEDLINE e LILACS até outubro de 2005. CONCLUSÃO: Os efeitos adversos metabólicos permanecem como problemas importantes da psicofarmacologia. Ganho de peso clinicamente relevante ocorre com freqüência em pacientes em uso de antipsicóticos e estabilizadores de humor, principalmente naqueles em uso de clozapina, olanzapina, lítio e ácido valpróico. A clozapina e a olanzapina associam-se também a uma maior incidência de diabetes melito e dislipidemias, seja devido ao ganho de peso, seja por ação deletéria direta sobre o metabolismo da glicose. A incidência de obesidade e outros distúrbios metabólicos é menor com a risperidona, se comparada à olanzapina ou à clozapina. Carbamazepina associa-se a menor ganho de peso, se comparada ao lítio ou ao ácido valpróico. Drogas como o haloperidol, a ziprasidona, o aripiprazol e a lamotrigina não estão associadas a ganho importante de peso ou a maior incidência de diabetes melito e são alternativas para pacientes mais propensos a desenvolver tais efeitos adversos.BACKGROUND: An increase in the incidence of obesity and diabetes mellitus in psychiatric patients using antipsychotic drugs was observed as early as the 1960's. In the 1980's and 1990's, rehabilitation of clozapine, synthesis of other atypical antipsychotics, and spread of the

  17. Glitazonas e síndrome metabólica: mecanismos de ação, fisiopatologia e indicações terapêuticas

    OpenAIRE

    Gomes, Marília de Brito

    2006-01-01

    O diabetes mellitus (DM) é considerado um problema de saúde pública em países devido às suas complicações crônicas macro e microvasculares, com grande impacto na morbimortalidade dos pacientes. A doença é o estágio final de uma síndrome crônica e progressiva, cujas anormalidades fisiopatológicas iniciam-se anos antes do diagnóstico clínico da doença. A síndrome metabólica (SM) é conseqüente ao aumento mundial da prevalência de obesidade. O DM é freqüentemente associado com condições clínicas ...

  18. Balanços metabólicos do enxofre em pacientes com leucodistrofia metacromática Metabolic balances of sulfur in patients with metachromatic leucodystrophy

    Directory of Open Access Journals (Sweden)

    Horacio M. Canelas

    1968-12-01

    Full Text Available Foram estudados os balanços metabólicos do enxofre em dois pacientes com a forma juvenil da leucodistrofia metacromática. Foi verificado, em ambos os casos, um balanço positivo desse metaloide, aparentemente não influenciado pelo tipo de dieta (geral ou vegetal. Este resultado está de acordo com a atual concepção patogênica dessa moléstia, que consiste, essencialmente, em uma sulfatidose com diminuição da atividade das sulfatases.The metabolic balances of sulfur in two cases of the late juvenile form of metachromatic leucodystrophy were studied. A positive balance of sulfur was found in both patients, apparently not influenced by the type of diet (either mixed or vegetarian. This finding is in accordance with the current views on the pathogenesis of the disease, namely a sulfatidosis with low sulfatase activity.

  19. Índice HOMA-Adiponectina como marcador substitutivo de resistência à insulina : Estudo Brasileiro de Síndrome Metabólica (BRAMS)

    OpenAIRE

    Brunna Sullara Vilela Rodrigues

    2016-01-01

    Resumo: Introdução: As principais consequências da obesidade estão diretamente relacionadas ao desenvolvimento da resistência à insulina (RI), especialmente a síndrome metabólica (SM) caracterizada como um conjunto de fatores de risco cardiovascular em um mesmo indivíduo. O índice HOMA-AD foi proposto recentemente como um método substitutivo de avaliação da resistência à insulina. Este método é uma modificação do índice HOMA-IR que acrescenta a informação de adiponectina sérica ao seu denomin...

  20. Prevalencia de Síndrome Metabólico en niños y adolescentes de América

    OpenAIRE

    Romain Pierlot; Estela Cuevas-Romero; Jorge Rodríguez-Antolín; Pablo Méndez-Hernández; Margarita Martínez-Gómez

    2017-01-01

    Objetivo: Analizar la prevalencia del Síndrome Metabólico (SM) y sus componentes en niños y adolescentes del continente Americano. Método: La recopilación de la información se tomó de artículos científicos de los años 2008 al 2016 que aparecen en las bases de datos PubMed, Europe PMC y SciELO. Se incluyeron estudios que muestran datos cuantitativos de los componentes del SM en niños y/o adolescentes entre 4 y 19 años de edad. Resultados: Se analizaron 23 estudios realizados en 12 países del c...

  1. Incidência de transtornos metabólicos subclínicos em bovinos leiteiros na região oeste de Santa Catarina

    OpenAIRE

    Eliana Lucia Fiorentin

    2016-01-01

    O manejo da vaca leiteira durante o período de transição tem sido objeto de estudo de diversas pesquisas devido às severas adaptações dos rebanhos animais ao metabolismo dos carboidratos, lipídeos e minerais no início da lactação. O objetivo deste estudo foi determinar a incidência de transtornos metabólicos subclínicos, dentre eles cetose, lipidose hepática, hipocalcemia, acidose ruminal, deficiência de fósforo, hipomagnesemia, e deficiência de cobre e zinco, em bovinos leiteiros da região O...

  2. Percepção do corpo, medo da morte, religião e doação de órgãos

    Directory of Open Access Journals (Sweden)

    Bendassolli Pedro Fernando

    2001-01-01

    Full Text Available O objetivo desta pesquisa foi o de levantar as principais razões que levam estudantes universitários a doarem seus órgãos para transplante e as relações entre a doação de órgãos, o medo da morte e a religião dos participantes. Para tanto, foram realizados três estudos interdependentes, os quais somaram a participação de 192 estudantes de uma universidade pública do Estado de São Paulo. Os resultados obtidos nestes estudos sugerem, como sendo as principais razões para a doação: desejo de continuar a vida do outro; reaproveitamento dos órgãos; dar qualidade de vida aos que necessitam de um transplante; inutilidade do corpo após a morte. Quanto à não doação, as principais razões foram: crítica à lei dos transplantes; crítica ao sistema de saúde brasileiro; razões bioéticas, tais como receio de morte premeditada e contrabando de órgãos. Nestes estudos não foi encontrada relação significativa entre religião e doação de órgãos, mas foi encontrada entre o medo da morte e a não doação.

  3. “Out of Germany”: Flossenbürg Concentration Camp, Jakub’s World (2005, and the Commemoration of the Holocaust in the United States

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    Klara Stephanie Szlezák

    2015-05-01

    Full Text Available This essay addresses survivor stories as formulations of Holocaust memory in the U.S. More specifically, it focuses on the former concentration camp at Flossenbürg in southern Germany. Compared to places like Dachau and Buchenwald in Germany or Auschwitz and Treblinka in Poland, Flossenbürg is often absent from or—if present at all—marginalized in the public and scholarly discourse of Holocaust memory. The heavily autobiographical novel Jakub’s World (2005 tells the story of Jakub Szabmacher, a Jewish boy who is taken from his home in Poland by the Nazis and is eventually interned at Flossenbürg. He survives many months of deprivation and hardship in the concentration camp until U.S. forces liberate it in April 1945; orphaned and homeless, he eventually relocates to the U.S., yet returns to the site of his suffering many times. Reading the book against the backdrop of ongoing debates about Holocaust memory in the U.S., this essay explores structural and plot elements in the book that complicate both a Jewish identity derived from victimhood and the notion of liberation as the moment of ultimate redemption. Both Jakub’s World and the Flossenbürg Concentration Camp Memorial as sites of memory actively partake in the commemoration of the Holocaust, offering narratives that not only complicate the notion of World War II as the “Good War” but also reveal the transnational dimension of memories of the Holocaust. Neither the stories and memories of survivors of the Holocaust at Flossenbürg, nor the visitors to the present-day memorial site, nor the characters central to the memoir can be framed, addressed, or understood in the context of national boundaries.

  4. Characterization of Chondrogenic Gene Expression and Cartilage Phenotype Differentiation in Human Breast Adipose-Derived Stem Cells Promoted by Ginsenoside Rg1 In Vitro

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    Fang-Tian Xu

    2015-11-01

    Full Text Available Background/Aims: Investigating and understanding chondrogenic gene expression during the differentiation of human breast adipose-derived stem cells (HBASCs into chondrogenic cells is a prerequisite for the application of this approach for cartilage repair and regeneration. In this study, we aim to characterize HBASCs and to examine chondrogenic gene expression in chondrogenic inductive culture medium containing ginsenoside Rg1. Methods: Human breast adipose-derived stem cells at passage 3 were evaluated based on specific cell markers and their multilineage differentiation capacity. Cultured HBASCs were treated either with basic chondrogenic inductive conditioned medium alone (group A, control or with basic chondrogenic inductive medium plus 10 µg/ml (group B, 50 µg/ml (group C, or 100µg/ml ginsenoside Rg1 (group D. Cell proliferation was assessed using the CCK-8 assay for a period of 9 days. Two weeks after induction, the expression of chondrogenic genes (collagen type II, collagen type XI, ACP, COMP and ELASTIN was determined using real-time PCR in all groups. Results: The different concentrations of ginsenoside Rg1 that were added to the basic chondrogenic inductive culture medium promoted the proliferation of HBASCs at earlier stages (groups B, C, and D but resulted in chondrogenic phenotype differentiation and higher mRNA expression of collagen type II (CO-II, collagen type XI (CO-XI, acid phosphatase (ACP, cartilage oligomeric matrix protein (COMP and ELASTIN compared with the control (group A at later stages. The results reveal an obvious positive dose-effect relationship between ginsenoside Rg1 and the proliferation and chondrogenic phenotype differentiation of HBASCs in vitro. Conclusions: Human breast adipose-derived stem cells retain stem cell characteristics after expansion in culture through passage 3 and serve as a feasible source of cells for cartilage regeneration in vitro. Chondrogenesis in HBASCs was found to be prominent

  5. Metabolic rates and thermoregulatory characteristics of Akodon azarae (Rodentia: Sigmodontinae Tasas metabólicas y características termorregulatorias de Akodon azarae (Rodentia: Sigmodontinae

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    C. DANIEL ANTINUCHI

    2000-03-01

    Full Text Available In this work, we evaluated the energetic status, activity daily metabolic rate, thermoregulatory capacity and the existence of torpor in Akodon azarae (Fisher 1829. Basal metabolic rate (BMR was similar to the expected value from Kleiber´s, and also according to the BMR of phylogenetically related species. No statistical difference in BMR between sexes was detected. For both males and females, activity daily metabolic rate was 235 % of BMR. Body temperature was not statistically different, neither at different ambient temperatures nor between sexes. In the thermoneutral zone, body temperature was 36.1 ± 1.5 °C. Thermal conductance was lower than the expected for mammals whereas thermoregulatory capacity was 136 % of the expected. Akodon azarae did not show evidence of torporEn este trabajo fueron evaluadas, para Akodon azarae (Fisher 1829, la tasa metabólica basal (BMR y de actividad diaria así como la capacidad termorregulatoria y la posible existencia de torpor. La BMR no presentó diferencias estadísticas con la esperada por la ecuación de Kleiber así como con la esperada para especies filogenéticamente relacionadas. Tampoco fueron detectadas diferencias significativas en la BMR entre sexos. La tasa metabólica de actividad diaria para ambos sexos fue el 235 % de la BMR. La temperatura corporal no presentó variaciones entre individuos mantenidos a diferentes temperaturas ambiente ni tampoco entre sexos y en termoneutralidad fue de 36,1 ± 1,5 °C. Por otra parte, la conductancia térmica fue menor a la esperada mientras que la capacidad termorregulatoria fue el 136% de la esperada. Akodon azarae no presenta sopor

  6. Prevalencia de Síndrome Metabólico en niños y adolescentes de América

    Directory of Open Access Journals (Sweden)

    Romain Pierlot

    2017-01-01

    Full Text Available Objetivo: Analizar la prevalencia del Síndrome Metabólico (SM y sus componentes en niños y adolescentes del continente Americano. Método: La recopilación de la información se tomó de artículos científicos de los años 2008 al 2016 que aparecen en las bases de datos PubMed, Europe PMC y SciELO. Se incluyeron estudios que muestran datos cuantitativos de los componentes del SM en niños y/o adolescentes entre 4 y 19 años de edad. Resultados: Se analizaron 23 estudios realizados en 12 países del continente americano, de los cuales sólo 3 consideraron el factor edad. Los componentes del SM más prevalentes fueron la obesidad y las dislipidemias. Mientras que los menos prevalentes fueron hiperglicemia e hipertensión. La hipoαlipoproteinemia, la hipertensión y el SM fueron más frecuentes en hombres que en mujeres. Pocos estudios analizaron factores de riesgo para el SM. Conclusión: La presencia del SM y sus componentes en niños y adolescentes americanos es variable. La variabilidad es también observada en países de otros continentes. Esta revisión evidencia la necesidad de definir los criterios de diagnóstico del SM en niños y jóvenes, y la urgencia de diseñar estrategias de prevención de las alteraciones metabólicas en edades tempranas, involucrando la participación de la familia, la sociedad y las instituciones públicas.

  7. Acercamiento a una problemática trascendente: el síndrome metabólico Approach to a transcendental problem: the metabolic syndrome

    Directory of Open Access Journals (Sweden)

    José Luis Valenciaga Rodríguez

    2005-04-01

    Full Text Available La presencia del síndrome metabólico (SM se relaciona con un incremento significativo de riesgo de diabetes, enfermedad coronaria y cerebrovascular, por lo que se exponen y analizan algunos aspectos fundamentales para su comprensión. Se responden interrogantes como: ¿qué es el síndrome metabólico y la insulinorresistencia?, ¿cuáles son sus causas?, ¿cuáles son las señales y/o signos clínicos de resistencia a la insulina que se pueden emplear para la detección por el Médico de Familia de casos con SM o en riesgo de padecerlo?, ¿cuáles son las clasificaciones que se han empleado para caracterizarlo?, ¿cuáles son los principales estudios dirigidos a disminuir el riesgo relativo de padecer diabetes mellitus tipo II (DM2 y SM? Se realizan algunas reflexiones para el enfrentamiento de esta problemática en la APS.As the presence of the metabolic syndrome (MS is related to a significant increase of risk for diabetes, coronary artery disease and cerebrovascular disease, some fundamental aspects for its understanding are exposed and analyzed. Answers are given to questions such as: what is the MS and the insulin resistance?, which are their causes?, which are the clinical signals or signs of insulin resistance the family physician may use to detect cases with MS or at risk for suffering from it?, which are the classifications used to characterize it?, and which are the main studies directed to reduce the relative risk for suffering from type II diabetes mellitus and MS? Some reflections are made to face this problem at the PHC level.

  8. Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07.

    Science.gov (United States)

    Gaur, Rajeeva; Tiwari, Soni

    2015-03-19

    The rising concerns about the scarcity of fossil fuels, the emission of green house gasses and air pollution by incomplete combustion of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. The aim of this study was to isolate a potential thermo-solvent tolerant cellulase producing bacterium from natural resources, and then applied for purification and characterization. The purified enzyme was to be accessible for the bioethanol production as well as industrial exploitation (discuss in our next study). It is the first instance when thermo-solvent tolerant cellulase producing bacterium was isolated from soil sample. The culture was identified as Bacillus vallismortis RG-07 by 16S rDNA sequence analysis. Bacillus vallismortis RG-07 reported maximum cellulase production from sugarcane baggase (4105 U ml(-1)) used as agro-waste carbon source. The cellulase enzyme produced by the Bacillus sp. was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography, with overall recovery of 28.8%. The molecular weight of purified cellulase was 80 kDa as revealed by SDS-PAGE and activity gel analysis. The optimum temperature and pH for enzyme activity was determined as 65°C and 7.0 and it retained 95 and 75% of activity even at 95°C, and 9.0 respectively. The enzyme activity was enhanced in the presence of organic solvents (30%) n-dodecane, iso-octane, n-decane, xylene, toluene, n-haxane, n-butanol, and cyclohexane, after prolonged incubation (7 days). The enzyme activity was also stimulated by Ca(2+), mercaptoethanol, Tween-60, and Sodium hypochloride whereas strongly inhibited by Hg. Kinetic analysis of purified enzyme showed the Km and Vmax to be 1.923 mg ml(-1) and 769.230 μg ml(-1) min(-1), respectively. The unique property of solvent-thermostable-alkalophilic, nature proves the potential candidature of this isolate for

  9. O órgão odorífero do macho de Athysania hesione Dry: (Lepidoptera, Noctuidae

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    Rudolf Barth

    1957-12-01

    Full Text Available O órgão odorífero de Athysania hesione (e outras espécies próximas está situado na face externa da segunda tíbia e compõe-se de uma região estreita tomada de células glandulares, situada no bordo posterior da tíbia cuja superfície externa forma um sulco longitudinal. Na parte proximal do núcleo insere-se um grande pincel distribuidor, cujas cerdas estão deitadas no sulco, onde entram em contato com as escamas odoríferas. Em virtude do enchimento de um saco traqueal, situado no interior da tíbia a pressão da hemolinfa aumenta, causando uma deformação da área de inserção do pincel que, por sua vez, sai do sulco, abrindo-se a fim de possibilitar a evaporação da secração. Depois da função, o pincel volta à posição de repouso graças à elasticidade da cutícula da área de inserção. O órgão é protegido por meio de séries de escamas protetoras de grandes superfícies. A célula glandular, excepcionalmente volumosa, localiza-se entre o sincício da hipoderme e a membrana basal (fig. 5. As células tricogêneas das cerdas do pincel distribuidor e das escamas protetoras possuíram, em épocas filogenéticas, função glandular; encontra-se, ainda hoje, no interior das primeiras, um aparelho excretor (fig. 9, sendo porém o seu núcleo e protoplasma completamente inativos, dando aos mesmo um aspecto de degenração.

  10. Chondroprotective Effects of Ginsenoside Rg1 in  Human Osteoarthritis Chondrocytes and a Rat Model  of Anterior Cruciate Ligament Transection

    Directory of Open Access Journals (Sweden)

    Wendan Cheng

    2017-03-01

    Full Text Available This study aimed to assess whether Ginsenoside Rg1 (Rg1 inhibits inflammatory responses in human chondrocytes and reduces articular cartilage damage in a rat model of osteoarthritis (OA. Gene expression and protein levels of type II collagen, aggrecan, matrix metalloproteinase (MMP‐13 and cyclooxygenase‐2 (COX‐2 were determined in vitro by quantitative real‐time‐polymerase chain reaction and Western blotting. Prostaglandin E2 (PGE2 amounts in the culture medium were determined by enzyme‐linked immunosorbent assay (ELISA. For in vivo assessment, a rat model of OA was generated by anterior cruciate ligament transection (ACLT. Four weeks after ACLT, Rg1 (30 or 60 mg/kg or saline was administered by gavage once a day for eight consecutive weeks. Joint damage was analyzed by histology and immunohistochemistry. Ginsenoside Rg1 inhibited Interleukin (IL‐1β‐induced chondrocyte gene and protein expressions of MMP‐13, COX‐2 and PGE2, and prevented type II collagen and aggrecan degradation, in a dose‐dependent manner. Administration of Ginsenoside Rg1 to OA rats attenuated cartilage degeneration, and reduced type II collagen loss and MMP‐13 levels. These findings demonstrated that Ginsenoside Rg1 can inhibit inflammatory responses in human chondrocytes in vitro and reduce articular cartilage damage in vivo, confirming the potential therapeutic value of Ginsenoside Rg1 in OA.

  11. Identification and characterization of a ginsenoside-transforming β-glucosidase from Pseudonocardia sp. Gsoil 1536 and its application for enhanced production of minor ginsenoside Rg2(S.

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    Juan Du

    Full Text Available The ginsenoside Rg2(S, which is one of the pharmaceutical components of ginseng, is known to have neuroprotective, anti-inflammation, and anti-diabetic effects. However, the usage of ginsenoside Rg2(S is restricted owing to the small amounts found in white and red ginseng. To enhance the production of ginsenoside Rg2(S as a 100 gram unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the recombinant glycoside hydrolase (BglPC28, which is a ginsenoside-transforming recombinant β-glucosidase from Pseudonocardia sp. strain Gsoil 1536. The gene, termed bglPC28, encoding β-glucosidase (BglPC28 belonging to the glycoside hydrolase family 3 was cloned. bglPC28 consists of 2,232 bp (743 amino acid residues with a predicted molecular mass of 78,975 Da. This enzyme was overexpressed in Escherichia coli BL21(DE3 using a GST-fused pGEX 4T-1 vector system. The optimum conditions of the recombinant BglPC28 were pH 7.0 and 37 °C. BglPC28 can effectively transform the ginsenoside Re to Rg2(S; the Km values of PNPG and Re were 6.36 ± 1.10 and 1.42 ± 0.13 mM, respectively, and the Vmax values were 40.0 ± 2.55 and 5.62 ± 0.21 µmol min-1 mg-1 of protein, respectively. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 7.0 and 30°C for 12 hours with a concentration of 20 mg/ml of ginsenoside Re from American ginseng roots. Finally, 113 g of Rg2(S was produced from 150 g of Re with 84.0 ± 1.1% chromatographic purity. These results suggest that this enzymatic method could be usefully exploited in the preparation of ginsenoside Rg2(S in the cosmetics, functional food, and pharmaceutical industries.

  12. 20(R)-ginsenoside Rg3, a rare saponin from red ginseng, ameliorates acetaminophen-induced hepatotoxicity by suppressing PI3K/AKT pathway-mediated inflammation and apoptosis.

    Science.gov (United States)

    Zhou, Yan-Dan; Hou, Jin-Gang; Liu, Wei; Ren, Shen; Wang, Ying-Ping; Zhang, Rui; Chen, Chen; Wang, Zi; Li, Wei

    2018-06-01

    Although ginsenoside Rg3 was isolated as a major component of Korea red ginseng and confirmed to exert potential hepatoprotective effect on acetaminophen (APAP)-induced liver injury via induction of glutathione S-transferase (GST) in vitro, thein vivo hepatoprotective effect of Rg3 and the underlying molecular mechanism of action remain unclear. The current study was aimed to explore whether 20(R)-Ginsenoside Rg3 (20(R)-Rg3) could alleviate acetaminophen-induced liver injury in mice and to determine the involvement of PI3K/AKT signaling pathway. Our findings demonstrated that a single injection of APAP (250 mg/kg) increased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β); such increases were attenuated by pretreatment of mice with 20(R)-Rg3 for seven days. The depletion of glutathione (GSH), generation of malondialdehyde (MDA) and the over expression of cytochrome P450 E1 (CYP2E1) and 4-hydroxynonenal (4-HNE) caused by APAP exposure were also inhibited by 20(R)-Rg3 pretreatment. Moreover, 20(R)-Rg3 pretreatment significantly alleviated APAP-induced apoptosis, necrosis, and inflammatory infiltration in liver tissues. Importantly, 20(R)-Rg3 effectively attenuated APAP-induced liver injury in part via activating PI3K/AKT signaling pathway. In summary, 20(R)-Rg3 exerted liver protection against APAP-caused hepatotoxicity evidenced by inhibition of oxidative stress and inflammatory response, alleviation of hepatocellular necrosis and apoptosis via activation of PI3K/AKT signaling pathway, showing potential as a novel therapeutic agent to prevent liver damage. Copyright © 2018 Elsevier B.V. All rights reserved.

  13. N-(4-Hydroxyphenyl) retinamide potentiated paclitaxel for cell cycle arrest and apoptosis in glioblastoma C6 and RG2 cells

    Science.gov (United States)

    Janardhanan, Rajiv; Butler, Jonathan T.; Banik, Naren L.; Ray, Swapan K.

    2009-01-01

    Glioblastoma grows aggressively due to its ability to maintain abnormally high potentials for cell proliferation. The present study examines the synergistic actions of N-(4-hdroxyphenyl) retinamide (4-HPR) and paclitaxel (PTX) to control the growth of rat glioblastoma C6 and RG2 cell lines. 4-HPR induced astrocytic differentiation was accompanied by increased expression of the tight junction protein e-cadherin and sustained down regulation of Id2 (member of inhibitor of differentiation family), catalytic subunit of rat telomerase reverse transcriptase (rTERT), and proliferating cell nuclear antigen (PCNA). Flow cytometric analysis showed that the microtubule stabilizer PTX caused cell cycle deregulation due to G2/M arrest. This in turn could alter the fate of kinetochore-spindletube dynamics thereby halting cell cycle progression. An interesting observation was induction of G1/S arrest by combination of 4-HPR and PTX, altering the G2/M arrest induced by PTX alone. This was further ratified by the upregulation of tumor suppressor protein retinoblastoma, which repressed the expression of the key signaling moieties to induce G1/S arrest. Collectively, combination of 4-HPR and PTX diminished the survival factors (e.g., rTERT, PCNA, and Bcl-2) to make glioblastoma cells highly prone to apoptosis with activation of cysteine proteases (e.g., calpain, cathepsins, caspase-8, caspase-3) in two glioblastoma cell lines. Hence, combination 4-HPR and PTX can be considered as an effective therapeutic strategy for controlling the growth of heterogeneous glioblastoma cell populations. PMID:19285047

  14. Photon activation therapy of RG2 glioma carrying Fischer rats using stable thallium and monochromatic synchrotron radiation.

    Science.gov (United States)

    Ceberg, Crister; Jönsson, Bo-Anders; Prezado, Yolanda; Pommer, Tobias; Nittby, Henrietta; Englund, Elisabet; Grafström, Gustav; Edvardsson, Anneli; Stenvall, Anna; Strömblad, Susanne; Wingårdh, Karin; Persson, Bertil; Elleaume, Hélène; Baldetorp, Bo; Salford, Leif G; Strand, Sven-Erik

    2012-12-21

    75 RG2 glioma-carrying Fischer rats were treated by photon activation therapy (PAT) with monochromatic synchrotron radiation and stable thallium. Three groups were treated with thallium in combination with radiation at different energy; immediately below and above the thallium K-edge, and at 50 keV. Three control groups were given irradiation only, thallium only, or no treatment at all. For animals receiving thallium in combination with radiation to 15 Gy at 50 keV, the median survival time was 30 days, which was 67% longer than for the untreated controls (p = 0.0020) and 36% longer than for the group treated with radiation alone (not significant). Treatment with thallium and radiation at the higher energy levels were not effective at the given absorbed dose and thallium concentration. In the groups treated at 50 keV and above the K-edge, several animals exhibited extensive and sometimes contra-lateral edema, neuronal death and frank tissue necrosis. No such marked changes were seen in the other groups. The results were discussed with reference to Monte Carlo calculated electron energy spectra and dose enhancement factors.

  15. Psychometric properties of the Slovenian adaptation of the Revised Generic Occupational Stress Index Questionnaire (RG-OSI

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    Nataša Sedlar

    2014-02-01

    Full Text Available The Revised Generic Occupational Stress Index questionnaire (RG-OSI employs the cognitive ergonomics approach that quantifies the burden of stressors on cognitive resources of the employee. The model is structured as a 2-dimensional matrix, where each element is scored from 0 to 2 (sometimes with intermediate values of 0.5, 1.5 or 1.75 as a combination of various items based on multiple criteria. Due to uncommon scoring system of the questionnaire, our study aimed to explore the appropriateness of the existing scoring system and to get some information on validity of the scale on a Slovenian sample. The questionnaire has been applied on 349 Slovenian employees from different occupational groups and the data were analysed by means of correspondence analysis, classical reliability and item analysis and item response theory analysis. The results of correspondence analysis demonstrate that the response categories on individual variables are not always ordered. Furthermore, we conducted reliability analysis for scales, developed short versions of the scales, and obtained some preliminary information on their validity. The current study provides evidence that the described original scoring system in psychological measures may not be appropriate from the psychometric viewpoint.

  16. U/Th dating by SHRIMP RG ion-microprobe mass spectrometry using single ion-exchange beads

    Science.gov (United States)

    Bischoff, J.L.; Wooden, J.; Murphy, F.; Williams, Ross W.

    2005-01-01

    We present a new analytical method for U-series isotopes using the SHRIMP RG (Sensitive High mass Resolution Ion MicroProbe) mass spectrometer that utilizes the preconcentration of the U-series isotopes from a sample onto a single ion-exchange bead. Ion-microprobe mass spectrometry is capable of producing Th ionization efficiencies in excess of 2%. Analytical precision is typically better than alpha spectroscopy, but not as good as thermal ionization mass spectroscopy (TIMS) and inductively coupled plasma multicollector mass spectrometry (ICP-MS). Like TIMS and ICP-MS the method allows analysis of small samples sizes, but also adds the advantage of rapidity of analysis. A major advantage of ion-microprobe analysis is that U and Th isotopes are analyzed in the same bead, simplifying the process of chemical separation. Analytical time on the instrument is ???60 min per sample, and a single instrument-loading can accommodate 15-20 samples to be analyzed in a 24-h day. An additional advantage is that the method allows multiple reanalyses of the same bead and that samples can be archived for reanalysis at a later time. Because the ion beam excavates a pit only a few ??m deep, the mount can later be repolished and reanalyzed numerous times. The method described of preconcentrating a low concentration sample onto a small conductive substrate to allow ion-microprobe mass spectrometry is potentially applicable to many other systems. Copyright ?? 2005 Elsevier Ltd.

  17. Doação de órgãos e tecidos: relação com o corpo em nossa sociedade

    OpenAIRE

    Roza,Bartira De Aguiar; Garcia,Valter Duro; Barbosa,Sayonara de Fátima Faria; Mendes,Karina Dal Sasso; Schirmer,Janine

    2010-01-01

    Este estudo, de revisão bibliográfica, objetivou tecer considerações teóricas sobre doação de órgãos e tecidos e sua relação com o corpo em nossa sociedade. O aumento da taxa de doação depende de um olhar ampliado além das questões técnicas do processo de doação de órgãos e tecidos. Vários países, com larga experiência temporal e, que trabalham sistematicamente nesse processo, incorporaram a abordagem social e a perspectiva ética, baseadas no voluntarismo das famílias e no respeito ao direito...

  18. Development and results of a test program to demonstrate compliance with IEEE STD 384 and R.G. 1.75 electrical separation requirements

    International Nuclear Information System (INIS)

    Eckert, G.P.; Heneberry, E.F.; Walker, F.P.; Konkus, J.F.

    1987-01-01

    The IEEE Std 384-1974, entitled ''Criteria for Separation of Class 1E Equipment and Circuits,'' contains criteria to ensure the independence of redundant Class 1E equipment when designing electrical systems in nuclear plants. The NRC, in R.G. 1.75 Rev. 2, 1978, endorses, with comments, IEEE-384, as the means of achieving independence. One method given in IEEE-384, is that of maintaining a specified separation between components; another method utilizes a combination of separation and barriers. The standard also allows alternative methods to be used when justified by test-based analyses. This paper is a report of a test program undertaken to provide a basis for analysis in the development of alternative methods of achieving separation. The test parameters developed and used, and the results obtained, should prove useful in determining alternative methods of complying with R.G. 1.75 requirements

  19. Escassez de órgãos e clonagem terapêutica: uma conexão possível?

    Directory of Open Access Journals (Sweden)

    Maria de Fátima Freire de Sá

    2017-01-01

    Full Text Available O presente artigo trata de dois assuntos polêmicos. O primeiro é a escassez de órgãos, problema em escala global que urge por soluções. O segundo é a clonagem terapêutica, técnica com perspectivas promissoras para a medicina regenerativa. É possível estabelecer uma conexão entre esses temas? Pode a clonagem terapêutica representar uma solução para o problema da escassez de órgãos? Para responder a essas perguntas deve-se passar por barreiras jurídicas e éticas. A travessia dessas barreiras será, portanto, o caminho percorrido ao longo deste artigo.

  20. Proteína C reativa e síndrome metabólica em crianças e adolescentes obesos e não obesos: relação com consumo alimentar

    OpenAIRE

    Borba, Vanessa Vieira Lopes

    2011-01-01

    A Síndrome Metabólica (SM) consiste em um conjunto de anormalidades metabólicas, descritas inicialmente em adultos, mas encontrada também na população pediátrica. As suas características englobam: obesidade, hipertensão arterial sistêmica, dislipidemia, intolerância à glicose e resistência insulínica. Todos esses fatores estão relacionados a questões genéticas, de comportamento alimentar e de hábitos de vida. A Proteína C Reativa (PCR), um marcador de atividade inflamatória, ve...

  1. Prevalencia y factores asociados al síndrome metabólico en niños y adolescentes obesos del municipio Marianao Prevalence and factors associated with metabolic syndrome in obese children and adolescents from Marianao municipality

    Directory of Open Access Journals (Sweden)

    Johandra Argote Parolis

    2008-12-01

    Full Text Available ANTECEDENTES: la obesidad y el síndrome metabólico constituyen un problema creciente de salud infantil a nivel mundial. OBJETIVO: determinar la prevalencia y factores asociados al síndrome metabólico en niños y adolescentes obesos del municipio Marianao. MÉTODOS: se evaluaron 460 estudiantes entre 8 y 15 años de edad. Se aplicó cuestionario que incluyó variables como: edad, sexo, color de la piel, antecedentes patológicos personales y familiares, peso, talla, tensión arterial (3 tomas e índice cintura-cadera, y se realizó prueba de tolerancia a la glucosa oral (0 y 2 h para medir glucemia e insulinemia, lipidograma y ácido úrico. Se consideró la presencia de síndrome metabólico teniendo en cuenta los criterios según propuesta cubana. Se obtuvieron distribuciones de frecuencia de las variables cualitativas y media, y desviación estándar de las cuantitativas. Se utilizó el paquete estadístico SPSS. RESULTADOS: la frecuencia de obesidad fue de 15 %, de ellos el 66 % presentó obesidad severa. La prevalencia de síndrome metabólico entre los obesos fue de 21,7 %, con elevada frecuencia entre 10 y 12 años, y no se observó en negros. Predominó el índice cintura-cadera >90 percentil sobre los demás criterios. La presencia de síndrome metabólico y la insulinorresistencia aumentaron con la severidad de la obesidad. Los antecedentes patológicos personales y familiares no mostraron relación con la presencia de síndrome metabólico, ni con el bajo peso al nacer, como tampoco con los índices de insulinorresistencia. CONCLUSIONES: la prevalencia de síndrome metabólico es notable, y se asocia a factores como la edad, el color de la piel y la severidad de la obesidad. El índice cintura-cadera presenta gran impacto sobre el síndrome metabólicoBACkgROUNDS: obesity and metabolic syndrome are an increasing problem of infantile health at world level. OBJETIVE: to determine prevalence and factors associated to metabolic

  2. [The Patient Rights Act (PatRG)--part 1: legislative procedure, treatment contract, contracting parties and their obligations to cooperate and inform].

    Science.gov (United States)

    Parzeller, Markus; Zedler, Barbara

    2013-01-01

    The article deals with the new regulations in the German Civil Code (BGB) which came into effect in Germany on 26 Feb 2013 as the Patient Rights Act (PatRG). In Part I, the legislative procedure, the treatment contract and the contracting parties (Section 630a Civil Code), the applicable regulations (Section 630b Civil Code) and the obligations to cooperate and inform (Section 630c Civil Code) are discussed and critically analysed.

  3. A reversion of an IL2RG mutation in combined immunodeficiency providing competitive advantage to the majority of CD8+ T cells.

    Science.gov (United States)

    Kuijpers, Taco W; van Leeuwen, Ester M M; Barendregt, Barbara H; Klarenbeek, Paul; aan de Kerk, Daan J; Baars, Paul A; Jansen, Machiel H; de Vries, Niek; van Lier, René A W; van der Burg, Mirjam

    2013-07-01

    Mutations in the common gamma chain (γc, CD132, encoded by the IL2RG gene) can lead to B(+)T(-)NK(-) X-linked severe combined immunodeficiency, as a consequence of unresponsiveness to γc-cytokines such as interleukins-2, -7 and -15. Hypomorphic mutations in CD132 may cause combined immunodeficiencies with a variety of clinical presentations. We analyzed peripheral blood mononuclear cells of a 6-year-old boy with normal lymphocyte counts, who suffered from recurrent pneumonia and disseminated mollusca contagiosa. Since proliferative responses of T cells and NK cells to γc -cytokines were severely impaired, we performed IL2RG gene analysis, showing a heterozygous mutation in the presence of a single X-chromosome. Interestingly, an IL2RG reversion to normal predominated in both naïve and antigen-primed CD8(+) T cells and increased over time. Only the revertant CD8(+) T cells showed normal expression of CD132 and the various CD8(+) T cell populations had a different T-cell receptor repertoire. Finally, a fraction of γδ(+) T cells and differentiated CD4(+)CD27(-) effector-memory T cells carried the reversion, whereas NK or B cells were repeatedly negative. In conclusion, in a patient with a novel IL2RG mutation, gene-reverted CD8(+) T cells accumulated over time. Our data indicate that selective outgrowth of particular T-cell subsets may occur following reversion at the level of committed T progenitor cells.

  4. Niños y adolescentes obesos con síndrome metabólico Obese children and adolescents with metabolic syndrome

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    José Raúl Zaldívar Ochoa

    2012-07-01

    Full Text Available Se realizó un estudio descriptivo y transversal de 200 niños y adolescentes obesos, con edades de 5 a 18 años, que acudieron a la consulta de Endocrinología del Hospital Docente Infantil Norte "Dr. Juan de la Cruz Martínez Maceira" de Santiago de Cuba, desde enero del 2008 hasta igual mes del 2010, a fin de determinar la existencia del síndrome metabólico en estos, para lo cual se consideraron las variables: edad, sexo, presencia de síndrome metabólico (según el National Cholesterol Education Program Adult Treatment Panel III, índice de masa corporal, bajo peso al nacer, antecedentes obstétricos de la madre (crecimiento intrauterino retardado, diabetes e hipertensión gestacionales y patológicos familiares (obesidad, diabetes mellitus e hipertensión arterial, así como parámetros bioquímicos. En la casuística se observó un bajo porcentaje del síndrome metabólico (12,0 %, con predominio en las edades de 15 a 18 años, el cual estuvo estrechamente relacionado con la circunferencia abdominal, que se presentó por encima de 95 percentil en 100,0 % de los pacientes; el bajo peso al nacer, que primó en 70,8 % de los afectados con la entidad clínica; y la severidad de la obesidad, que mostró la importante cifra de 95,8 % en la génesis de dicho síndrome.A descriptive and cross-sectional study was conducted in 200 obese children and adolescents between 5 and 18 years of age that attended the Endocrinology Department of «Dr. Juan de la Cruz Martínez Maceira» Northern Teaching Children Hospital in Santiago de Cuba, from January 2008 to January 2010, in order to determine the occurrence of metabolic syndrome in these patients, for which the variates such as age, sex, presence of metabolic syndrome (according to the National Cholesterol Education Program Adult Treatment Panel III, and body mass index, low birth weight, mother's reproductive history (intrauterine growth retardation, gestational diabetes and hypertension, family

  5. Síndrome metabólico en niños y adolescentes hipertensos obesos Metabolic syndrome in hypertensive and obese children and adolescents

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    Yudalvis Oquendo de la Cruz

    2010-12-01

    Full Text Available INTRODUCCIÓN. La combinación de hipertensión y obesidad en la infancia y la adolescencia incrementa el riesgo de un síndrome metabólico (SM en edades tempranas de la vida. El objetivo de este estudio fue determinar la presencia de SM en adolescentes hipertensos obesos y su relación con algunas variantes clínicas y bioquímicas. MÉTODOS. Se realizó un estudio descriptivo y prospectivo, con 152 niños y adolescentes hipertensos obesos, con edades entre 5 y 19 años, procedentes de la consulta de nefrología del Hospital Pediátrico Universitario «Juan Manuel Márquez». Se realizaron estudios de microalbuminuria, glucemia, insulina, colesterol total y triglicéridos en ayunas. RESULTADOS. Se encontró síndrome metabólico en el 35,5 % de los hipertensos obesos y fue más frecuente en el sexo masculino. Predominó la obesidad grave y 8 de cada 10 niños presentaron 3 criterios del síndrome. La prevalencia de cada uno de los componentes fue del 33,3 % en la alteración del metabolismo de la glucosa, del 100 % en la obesidad central e hipertensión arterial, del 51,8 % en la hipertrigliceridemia y del 13 % en el colesterol de lipoproteínas de alta densidad (HDL-C bajo. Casi la mitad de los pacientes tenían microalbuminuria. El 31,5 % de los sujetos estudiados presentó insulinorresistencia y en el 29,5 % de los casos se encontró hígado graso no alcohólico. CONCLUSIONES.Uno de cada 3 hipertensos obesos tenía síndrome metabólico, por lo que se recomienda continuar investigando sobre este síndrome en la infancia y la adolescencia.INTRODUCTION. The combination of high blood pressure and obesity in childhood increases the risk of metabolic syndrome (MS in early ages of life. The aim of present study was to determine the presence of MS in obese hypertensive adolescents and its relation to some clinical and biochemical variants. METHODS. A prospective and descriptive study was conducted in 152 obese children and adolescents aged 5 and

  6. Indicadores metabólicos en razas lecheras especializadas en condiciones tropicales en Colombia Metabolic profile in dairy cows under tropical conditions in Colombia

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    Rómulo Campos G

    2007-06-01

    Full Text Available Se ha seleccionado un alto número de razas bovinas para producción de leche, sin embargo, las de origen Bos taurus no han logrado adaptarse a las condiciones tropicales. El objetivo del trabajo fue analizar el comportamiento metabólico de siete razas (Ayrshire, Girolando, Holstein Friesian, Jersey, Lucerna, Pardo Suizo y Simenthal a través de 15 metabolitos. Se emplearon 28 animales por raza, distribuidos en cuatro grupos fisiológicos: novillas, inicio y final de lactancia y vacas secas (final de gestación. Los valores medios de los indicadores metabólicos fueron: BOH 0.5 mmol/l; glucosa 2.8 mmol/l; colesterol 2.5 mmol/l; potasio 4.1 mmol/l; calcio 2.0 mmol/l; fósforo inorgánico 1.7 mmol/l; magnesio 1.1 mmol/l; proteínas totales 66.2 mg/dl; albúmina 25.8 mg/dl; globulinas 40.2 mg/dl; creatinina 109 µmol/l; BUN 3.8 mmol/l; ALT 32.2 UI/l; AST 56.6 UI/l; GGT 12.3 UI/l; bilirrubina total 0.2 µmol/l; bilirrubina conjugada 0.08 µmol/l. Los valores medios de la condición corporal y el hematocrito fueron 3.25% y 27.0% respectivamente. Se encontraron diferencias estadísticas significativas entre grupos raciales y entre grupos de producción. Los animales de menor peso metabólico (Jersey presentaron mejor homeostasis que los de pesos mayores (Simenthal, Holstein.High numbers of bovine breeds have been selected as milk producers, but those derived from the Bos taurus breeds have been unable to adapt to tropical conditions. The aim of this work was to analyze the metabolic profile of the seven breeds (Ayrshire, Girolando, Holstein Friesian, Jersey , Lucerna, Brown Swiss and Simmental through the use of 15 metabolites. For each breed, 28 animals were used, divided into four physiological different groups: heifers, cows from the first stages and last stages of the lactation process and dry cows. The mean values of metabolites indicators were as follow: BOH 0.5 mmol/l; glucose 2.8 mmol/l; cholesterol 2.5 mmol/l; potassium 4.1 mmol/l; calcium 2

  7. Metabolic profile in dairy cows under tropical Indicadores metabólicos en razas lecheras especializadas en condiciones tropicales en Colombia

    Directory of Open Access Journals (Sweden)

    Campos G. Rómulo

    2007-06-01

    Full Text Available High numbers of bovine breeds have been selected as milk producers, but those derived from the Bos taurus breeds have been unable to adapt to tropical conditions. The aim of this work was to analyze the metabolic profile of the seven breeds (Ayrshire, Girolando, Holstein Friesian, Jersey, Lucerna, Brown Swiss and Simmental through the use of 15 metabolites. For each breed, 28 animals were used, divided into four physiological different groups: heifers, cows from the first stages and last stages of the lactation process and dry cows. The mean values of metabolites indicators were as follow: BOH 0.5 mmol/l; glucose 2.8 mmol/l; cholesterol 2.5 mmol/l; potassium 4.1 mmol/l; calcium 2.0 mmol/l; inorganic phosphorus 1.7 mmol/l; magnesium 1.1 mmol/l; total protein 66.2 mg/dl; albumin 25.8 mg/dl; globulin 40.2 mg/dl; creatinine 109 µmol/l; BUN 3.8 mmol/l; ALT 32.2 UI/l; AST 56.6 UI/l; GGT 12.3 UI/l; total bilirubin 0.2 µmol/L; conjugate bilirubin Additionally, body condition and PCV were determined with average values of 3.25 and 27% respectively. A significant statistical difference was found among breeding groups and physiological groups for production. It was observed that those animals with a low metabolic weight (Jersey demonstrate better homeostasis than those of high metabolic weight (Simmental, Holstein.Se ha seleccionado un alto número de razas bovinas para producción de leche, sin embargo, las de origen Bos taurus no han logrado adaptarse a las condiciones tropicales. El objetivo del trabajo fue analizar el comportamiento metabólico de siete razas (Ayrshire, Girolando, Holstein Friesian, Jersey, Lucerna, Pardo Suizo y Simenthal a través de 15 metabolitos. Se emplearon 28 animales por raza, distribuidos en cuatro grupos fisiológicos: novillas, inicio y final de lactancia y vacas secas (final de gestación. Los valores medios de los indicadores metabólicos fueron: BOH 0.5 mmol/l; glucosa 2.8 mmol/l; colesterol 2.5 mmol/l; potasio 4

  8. Sedentarismo e variáveis clínico-metabólicas associadas à obesidade em adolescentes Inactivity and clinical and metabolic variables associated with adolescent obesity

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    Priscila Trapp Abbes

    2011-08-01

    Full Text Available OBJETIVO: Estudar a associação da obesidade com variáveis metabólicas, variáveis clínicas e sedentarismo, em adolescentes pós-púberes de escolas públicas de São Paulo. MÉTODOS: Estudo caso-controle com 128 adolescentes obesos (índice de massa corporal > percentil 95 e 151 adolescentes eutróficos (índice de massa corporal entre percentis 5 e 85. Foram realizados exame físico, avaliação bioquímica e de composição corporal. Foi aplicado questionário previamente testado, que gerou um escore de sedentarismo. Na análise estatística, foi realizada a análise de variância com testes de comparações múltiplas de Bonferroni e qui-quadrado (Pearson. O modelo logístico múltiplo foi utilizado para verificar as associações entre variáveis clínicas, variáveis metabólicas, escore de sedentarismo e estado nutricional. RESULTADOS Por meio da análise de variância, foi identificado um gradiente nos valores médios das variáveis metabólicas e clínicas com piora dessas variáveis em paralelo ao aumento do grau de sedentarismo, o que foi confirmado pelo teste qui-quadrado. Na análise bivariada de riscos para obesidade, os adolescentes obesos apresentaram maiores frequências de sedentarismo, de alterações nos níveis de lipoproteína de alta densidade e triglicérides, de hiperinsulinemia e homeostasis model assessment for insulin resistence alterado, e de pressão arterial alterada (pOBJECTIVE: This study investigated the association of obesity with metabolic and clinical variables and inactivity in post-pubertal adolescents attending public schools in São Paulo City. METHODS: This was a case-control study with 128 obese adolescents (body mass indices >the 95th percentile, and 151 normal weight adolescents (body mass indices between the 5th and 85th percentiles. Physical examination and biochemical and body composition assessments were done. A pretested questionnaire was administered, generating an inactivity score. Analysis

  9. Prevalencia del síndrome metabólico en El Retiro, Colombia Metabolic syndrome prevalence in El Retiro, Colombia

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    Mónica María Toro L.

    2003-03-01

    Full Text Available Objetivos: establecer la prevalencia del síndrome metabólico y sus componentes en la población urbana del municipio de El Retiro, localizado en el departamento de Antioquia, noroccidente de Colombia utilizando los criterios del ATP III. Materiales y Métodos: del total de la población mayor de 20 años mediante un muestreo aleatorio por conglomerados se evaluaron 381 sujetos, a quienes se les realizó una encuesta sistematizada y se les tomó la presión arterial en el brazo derecho sentados, el peso en kilogramos, la talla en centímetros y las circunferencias de cintura y cadera. A 365 sujetos en ayunas se les determinó la glucemia, colesterol total, triglicéridos, colesterol HDL y por fórmula de Friedewald el colesterol LDL. El análisis estadístico se realizó mediante EPI-INFO y STATA 6.0 tomando como significativa una p < 0.05. Resultados: la prevalencia ajustada a la edad encontrada para cada uno de los factores fue la siguiente: hiperglucemia (Glucemia > 110 mg/dl o diabetes previa 12.5%, hipertrigliceridemia (TG >150 mg/dl 32.16%, disminución del colesterol HDL (< 40 mg/dl 38.87%, hipertensión (PA > 130/85 mmHg o hipertensión previa 48.58%, circunferencia cintura (H > 102 cm, M > 88 cm 27.6%. La metabólico según los criterios de ATP III por la presencia de 3 ó más de sus componentes fue de 23.64%. Conclusiones Conclusiones: el síndrome metabólico tiene la alta prevalencia de (23% en El Retiro Antioquia. Debido a su impacto en la salud de la población y a que la piedra angular en su tratamiento es disminuir de peso y aumentar la actividad física, se deberían plantear estrategias para lograr estos objetivos. Objetive: to establish the prevalence of the metabolic syndrome and its components using ATPIII criteria in the urban population of El Retiro, located in the Antioquia department, in the northwestern region of Colombia. Materials and methods: 381 subjects were evaluated from the total population older than 20, by

  10. Asociación entre el antecedente de nacido grande para la edad gestacional y el desarrollo de síndrome metabólico a lo largo de la vida: Revisión de la literatura

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    Ivana Nieto

    2016-06-01

    Full Text Available El síndrome metabólico se caracteriza por la presencia de obesidad abdominal, triglicéridos séricoselevados, HDL bajo, tensión arterial alta y glicemia en ayunas alta. Se ha asociado el desarrollo de síndromemetabólico a lo largo de la vida con el antecedente de haber nacido grande para la edad gestacional(GEG. Existe amplia evidencia del impacto negativo sobre la salud y calidad de vida de la poblaciónque desarrolla síndrome metabólico. En esta revisión, se describe la asociación entre el antecedente dehaber nacido GEG y el desarrollo de síndrome metabólico a lo largo de la vida, a partir de la mediciónde diferentes parámetros antropométricos (índice de masa corporal –IMC–, perímetro abdominal, peso ytalla y biomarcadores séricos (Factor de necrosis tumoral Alfa-TNF alfa-, Homeostasis Model AssessmentIndex –HOMA IR–, perfil lipídico, leptina y adiponectina en alguna etapa de la vida de personas conantecedente de haber nacido GEG comparadas con aquellas nacidas con peso adecuado para la edadgestacional (PAEG. Se concluye que tanto los parámetros antropométricos como los biomarcadoresmencionados, presentan diferencias significativas entre los pacientes con antecedente de haber nacidoGEG y los nacidos PAEG. Se requiere además ahondar en otros parámetros diferentes a los mencionados,como la tensión arterial y predisponentes genéticos con estudios que permitan mayor tiempo deseguimiento para establecer una asociación más contundente entre el antecedente de haber nacido GEGy el desarrollo de síndrome metabólico posterior.

  11. Doação de órgãos: meu corpo, minha sociedade Organ donation: my body, my society

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    Pedro Fernando Bendassolli

    1998-01-01

    Full Text Available A doação de órgãos é um problema de relevância social e científica, pois envolve as formas como a vida é perpetuada através do corpo de outra pessoa, especificamente por meio de seus órgãos. Quais são os fatores de resistência e de facilitação à doação de órgãos? Este artigo propõe um fator de resistência e um de facilitação. O primeiro, o narcisismo pelo próprio corpo; o segundo, o desejo de perpetuar-se a si mesmo e a vida.Organ donation is a problem of social and scientific relevance because it involves the ways through which life is perpetuated across another person’s body, specifically by its organs. What are resistance and facilitation factors to organ donation? This article proposes one factor of resistance and one of facilitation. The first, the narcissism of the own body; the second, the desire of perpetuate oneself and life.

  12. Evaluation of glucosidases of Aspergillus niger strain comparing with other glucosidases in transformation of ginsenoside Rb1 to ginsenosides Rg3

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    Kyung Hoon Chang

    2014-01-01

    Full Text Available The transformation of ginsenoside Rb1 into a specific minor ginsenoside using Aspergillus niger KCCM 11239, as well as the identification of the transformed products and the pathway via thin layer chromatography and high performance liquid chromatography were evaluated to develop a new biologically active material. The conversion of ginsenoside Rb1 generated Rd, Rg3, Rh2, and compound K although the reaction rates were low due to the low concentration. In enzymatic conversion, all of the ginsenoside Rb1 was converted to ginsenoside Rd and ginsenoside Rg3 after 24 h of incubation. The crude enzyme (β-glucosidase from A. niger KCCM 11239 hydrolyzed the β-(1→6-glucosidic linkage at the C-20 of ginsenoside Rb1 to generate ginsenoside Rd and ginsenoside Rg3. Our experimental demonstration showing that A. niger KCCM 11239 produces the ginsenoside-hydrolyzing β-glucosidase reflects the feasibility of developing a specific bioconversion process to obtain active minor ginsenosides.

  13. Agregação de fatores de risco metabólicos, atividade física e comportamentos de risco à saúde em adolescentes: revisão da literatura

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    Michael Pereira da Silva

    2014-12-01

    Full Text Available Objetivo: apresentar dados sobre a associação da atividade física, sedentarismo, alimentação inadequada, consumo de cigarro e bebidas alcoólicas com a agregação de fatores de risco metabólicos em adolescentes. Método: estudo de revisão utilizando critérios de diagnóstico de Sindrome Metabólica em crianças e adolescentes. Resultados e considerações finais: comportamentos como a atividade física, comportamento sedentário e hábitos alimentares possuem sua associação melhor elucidada na literatura, quando comparados aos hábitos de fumo e consumo de álcool, os quais necessitam de maiores investigações nesta população. Contudo, evidências demonstram que todos os hábitos abordados neste trabalho possuem alguma relação com a agregação de fatores de risco metabólicos na idade adulta, e sua prevenção na adolescência parece favorecer o não aparecimento dos mesmos no futuro.

  14. Inter-relação entre síndrome metabólica, estresse crônico e ritmos circadianos de marcadores adipogênicos: uma revisão

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    Carla de Oliveira

    2013-12-01

    Full Text Available Alterações de ritmos circadianos são relacionadas ao desenvolvimento de transtornos metabólicos como obesidade e síndrome metabólica. Além disto, a disponibilidade e o consumo de alimentos altamente palatáveis ricos em gordura e a exposição a situações estressantes podem contribuir na incidência e na severidade destas doenças crônicas, provocando modificações no balanço energético, alterações no metabolismo de lipídeos e adipogênese. Nesta revisão, abordaremos os aspectos cronobiológicos que podem ser influenciados pela exposição a estresse crônico e a obesidade, além dos mecanismos que contribuem para o crescimento de doenças metabólicas.

  15. Resistência insulínica e sua relação com os componentes da síndrome metabólica

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    Carla Campos Muniz Medeiros

    2011-11-01

    Full Text Available FUNDAMENTO: Os portadores de resistência à insulina apresentam maior predisposição para desenvolver posteriormente Síndrome Metabólica (SM, Diabetes Mellitus tipo 2 e Doença Cardiovascular (DCV. OBJETIVO: Avaliar a associação entre resistência insulínica (RI e os componentes da síndrome metabólica. MÉTODOS: Estudo transversal envolvendo 196 indivíduos entre 2 e 18 anos, usuários do sistema único de saúde. A associação da RI com os componentes da SM foi avaliada pelo teste do qui-quadrado, adotando-se o valor do índice da homeostase glicêmica (HOMA-RI > 2,5, e pelo teste de variância (ANOVA e Tukey, por meio da comparação das médias dos componentes nos quartis do HOMA-RI. A análise estatística foi realizada através do SPSS 17.0, com a adoção do nível de significância de 5%. RESULTADOS: RI foi observada em 41,3% da população estudada e esteve associada à faixa etária entre 10-18 anos (p = 0,002 RP = 3,2, à SM em ambos os sexos [Masculino (p = 0,022 RP = 3,7 e Feminino (p = 0,007 RP = 2,7] e ao triglicerídeo alterado (p = 0,005 RP = 2,9 no sexo feminino. Os valores médios dos componentes da SM diferiram significativamente entre os quartis do HOMA-RI (p < 0,01, com exceção do HDL-colesterol. CONCLUSÃO: A resistência insulínica pode ser considerada um marcador de risco cardiovascular.

  16. Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica

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    Rosecler Riethmuller Franco

    2011-02-01

    Full Text Available FUNDAMENTO: A síndrome metabólica está associada ao aumento de risco de eventos cardiovasculares. Marcadores inflamatórios e anticorpos anti-Chlamydia têm sido relacionados ao desenvolvimento e à progressão da aterosclerose e dos eventos cardiovasculares. OBJETIVO: Avaliar os marcadores inflamatórios interleucina-6 (IL-6 e fator de necrose tumoral-alfa (TNF-α e os anticorpos anti-Chlamydia pneumoniae em pacientes com síndrome metabólica (SM, com e sem eventos cardiovasculares. MÉTODOS: Estudo transversal constituído por 147 indivíduos. Desses, 100 (68% com SM e sem eventos cardiovasculares; e 47 (32% com SM e com eventos cardiovasculares. Dos indivíduos que sofreram eventos cardiovasculares, 13 (6,11% apresentam infarto agudo do miocárdio (IAM, e dez (4,7%, acidente vascular cerebral (AVC. O diagnóstico da SM foi determinado pelos critérios do NCEP-ATPIII. RESULTADOS: A média de idade dos sujeitos com eventos cardiovasculares foi de 61,26 ± 8,5 e de 59,32 ± 9,9 nos indivíduos sem esses eventos (p=0,279, havendo predomínio do sexo feminino. O grupo com SM e sem evento apresentou maior peso, altura, IMC e circunferência abdominal. Para os indivíduos com eventos cardiovasculares (p=0,001, os marcadores inflamatórios IL-6 e TNF-α e a doença vascular periférica foram significativamente maiores. Obtiveram-se níveis elevados de anticorpos IgG para Chlamydia pneumoniae no grupo SM, sem eventos e de IgA no grupo com eventos quando comparados os dois grupos. Com relação ao IAM e ao AVC, os anticorpos anti-Chlamydia pneumoniae não demonstraram significância estatística, comparados ao grupo sem eventos cardiovasculares. Associação foi observada com o uso de estatinas, hipoglicemiantes orais, injetáveis e anti-inflamatórios não esteroidais no grupo com esses eventos. CONCLUSÃO: Marcadores inflamatórios encontram-se significativamente elevados em pacientes com SM, com IAM e AVC. Anticorpos anti-Chlamydia n

  17. Intervenções nutricionais em Síndrome Metabólica: uma revisão sistemática

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    Leila Sicupira Carneiro de Souza Leão

    2011-09-01

    Full Text Available Não existe consenso sobre a estratégia nutricional mais adequada para tratar a Síndrome Metabólica (SM, de tal forma que ocorra redução do risco cardiovascular. O presente estudo foi desenhado para avaliar a força de evidência dos benefícios de diferentes intervenções nutricionais na remissão da SM. A busca virtual foi realizada nas bases de dados Medline, Cochrane Library e PubMed, de ensaios clínicos randomizados publicados no período entre 1999 a 2009, em qualquer língua, em indivíduos com 18 anos ou mais e diagnóstico de SM, independente do critério. O operador booleano and foi utilizado na combinação dos MeSH terms "Metabolic Syndrome", "Síndrome x Metabólica" e "Metabolic Syndrome X"; dos entry terms "Dysmetabolic Syndrome X", Metabolic Cardiovascular Syndrome", "Metabolic X Syndrome", "Syndrome X, Metabolic" adicionados dos termos "diet", "intervention and diet", "treatment and diet" e "supplementation". Para cada estudo incluído na revisão foi estimada a Prevalência de SM e o Cálculo da Eficácia após o período de seguimento. Medidas de risco relativo para cada estudo foram descritas pelo Forest Plot. Foram identificados 131 artigos, os quais após critérios de elegibilidade resultaram em 15 estudos. Estes foram separados em quatro grupos: dieta normocalórica associada a exercícios; dieta normocalórica isolada; dieta hipocalórica associada a exercícios; e dieta hipocalórica isolada. Os ensaios com dieta hipocalórica associada à prática de exercícios apresentaram valores mais elevados de eficácia, colaborando para ressaltar os aspectos globais da mudança do estilo de vida no tratamento da SM, onde a alimentação saudável e reduzida em calorias deve ser complementada com a prática de atividade física.

  18. Effect of plant and fungous metabolites on Meloidogyne exigua Efeito de metabólitos vegetais e fúngicos sobre Meloidogyne exigua

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    Daniel Rufino Amaral

    2009-01-01

    mencionada no trabalho anterior, bem como metabólitos na forma bruta de Fusarium moniliforme Shelden e Cylindrocarpon magnusianum (Sacc. Woll., produzidos em meio de cultura líquido, foram submetidos a testes in vitro com juvenis do segundo estádio (J2 de M. exigua. Todas as plantas e fungos produziram substâncias ativas contra J2. Portanto, seus metabólitos foram aplicados em mudas de café de seis meses de idade, inoculadas com M. exigua. Após 90 dias em casa de vegetação, verificou-se que os materiais oriundos de A. cepa, L. leucocephala, R. graveolens e F. moniliforme inibiram a produção de ovos e de galhas por M. exigua, demonstrando potencial para o controle de tal parasita.

  19. Intervenção nutricional e o impacto na adesão ao tratamento em pacientes com síndrome Metabólica

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    Fernanda Michielin Busnello

    2011-09-01

    Full Text Available FUNDAMENTO: A síndrome metabólica representa um conjunto de fatores de risco, associados a doenças cardiovasculares e a diabete melito tipo 2. O tratamento inclui mudanças no estilo de vida, dieta, atividade física e medicamentos. A adesão do paciente é crucial ao tratamento da doença. Objetivo: Avaliar dois modelos de intervenção dietoterápica e a relação com a adesão ao tratamento e o impacto na melhora clínica de pacientes com síndrome metabólica. MÉTODOS: Ensaio clínico randomizado, com duração de quatro meses. Os pacientes foram randomizados em grupos intervenção e controle. Todos seguiram dieta específica por quatro meses. O grupo Intervenção recebeu pacote de intervenção, com dieta individualizada, manual de orientação, aconselhamento via telefone e material educativo. Foram realizadas anamnese nutricional, avaliação antropométrica, avaliação dietética, orientação dietética individualizada e exames bioquímicos. RESULTADOS: Os pacientes que chegaram mais motivados foram aqueles que tiverem maior redução nos valores do índice de massa corporal (p < 0,001, que reduziu de 31,7 kg/m² (DP ± 3,9 para 30,9 kg/m² (DP ± 3,8, na circunferência abdominal a redução foi de 108,1 cm (DP ± 9,8 para 105,9 cm (DP ± 9,5. As associações estatisticamente significativas se deram nas correlações entre índice de massa corporal, glicemia e triglicerídeos, redução do consumo de leite integral (p = 0,002, aumento no consumo de cereais integrais (p = 0,008 e de leite desnatado (p = 0,010, e entre o aumento no consumo de vegetais e a redução dos triglicerídeos. CONCLUSÃO: Ambos os grupos mostraram melhora significativa nos parâmetros clínicos, que foi significativamente associada a motivação prévia. Os pacientes que chegaram mais motivados foram aqueles que responderam melhor ao tratamento.

  20. Hábitos Alimentares, Atividade Física e Escore de Risco Global de Framingham na Síndrome Metabólica

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    Thays Soliman Soares

    2014-04-01

    Full Text Available Fundamento: A síndrome metabólica é um transtorno complexo representado por um conjunto de fatores de risco cardiovascular. A adoção de um estilo de vida saudável está fortemente relacionada à melhora da Qualidade de Vida e interfere de forma positiva no controle dos fatores de risco presentes nessa condição clínica. Objetivo: Avaliar o efeito de um programa de modificação do estilo de vida sobre o Escore de Risco Cardiovascular Global de Framingham em indivíduos com síndrome metabólica. Método: Trata-se de uma subanálise de um ensaio clínico randomizado, controlado, cegado, com duração de três meses. Os participantes foram randomizados em quatro grupos: intervenção nutricional + placebo (INP, intervenção nutricional + suplementação de ácidos graxos ômega 3 (3 g/dia de óleo de peixe (INS3, intervenção nutricional + atividade física + placebo (INEP e intervenção nutricional + atividade física + suplementação de ácidos graxos ômega 3 (INES3. O Escore de Risco Cardiovascular Global de Framingham de cada indivíduo foi calculado antes e após a intervenção. Resultados: Participaram do estudo 70 indivíduos. Observou-se uma redução da média do escore após a intervenção de forma geral (p < 0,001. Obteve-se uma redução para risco intermediário em 25,7% dos indivíduos. Após a intervenção, observou-se redução significativa (p < 0,01 da "idade vascular", sendo esta mais expressiva nos grupos INP (5,2% e INEP (5,3%. Conclusão: Todas as intervenções propostas produziram efeito benéfico para a redução do escore de risco cardiovascular. O presente estudo reforça a importância da modificação do estilo de vida na prevenção e no tratamento das doenças cardiovasculares.

  1. Modificaciones hemodinámicas y metabólicas maternas secundarias al uso de betametasona para la maduración pulmonar fetal

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    Keibis Jiménez-Castillejo

    2013-09-01

    Full Text Available El objetivo de la investigación fue determinar las modificaciones hemodinámicas y metabólicas maternas secundarias al uso de betametasona para la maduración pulmonar fetal. Se realizó una investigación de tipo explicativa, prospectiva y longitudinal con un diseño cuasi-experimental y una muestra no probabilística intencional de 106 pacientes que acudieron a la consulta pre-natal de alto riesgo del Hospital Central “Dr. Urquinaona". Una vez seleccionadas las pacientes se le administró inyecciones intramusculares de betametasona (12 mg por dos días consecutivos. El valor de presión arterial sistólica presentó un leve aumento luego de la segunda medición y la tercera medición (p = ns. La presión arterial diastólica presentó aumento significativo durante la segunda medición y un nuevo aumento significativo en la tercera medición (p < 0,05. Se observaron aumentos significativos en el valor promedio de la frecuencia cardiaca luego de la segunda y tercera medición de betametasona (p < 0,05. No se encontraron modificaciones significativas en las concentraciones de glicemia, sodio y potasio entre las tres mediciones (p = ns. Se concluye que el uso de betametasona para inducir la maduración pulmonar fetal produce modificaciones hemodinámicas maternas acompañados de cambios metabólicos transitorios. Hemodynamical and metabolic modifications secondary to betamethasone use for fetal lung maturity Abstract The objective of research was to determine maternal hemodynamic and metabolic modifications secondary to the use of betamethasone for fetal lung maturation. An explicative, prospective and longitudinal research was done with a quasi-experimental design and intentional non probabilistic sample of 106 patients who assisted to High Risk Antenatal Consult at Hospital Central “Dr. Urquinaona". Once patients were selected, two intramuscular injections of betamethasone (12 mg were administered for two consecutive days. Systolic blood

  2. Cell size and basal metabolic rate in hummingbirds Tamaño celular y tasa metabólica basal en picaflores

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    Juan C. Opazo

    2005-06-01

    Full Text Available Nucleotypic theory suggests that genome size play indirect roles in determining organismal fitness. Among endotherms this theory has been demonstrated by an inverse correlation between basal metabolic rate (BMR and genome size. Nonetheless, accumulation of variables, especially for some key groups of endotherms, involved in C-value enigma (e.g., cell size will fortify this theory. In this sense, hummingbird species are of particular interest because they are an energetic extreme in avian and endotherm evolution. Knowing that cell size is proportional to C-value, in this study we tested for a relationship between mean corpuscular volume of red blood cells and BMR in four species of hummingbirds ranging from 4 to 20 g. In comparison with other birds, our hummingbird data show higher BMR and the smallest mean corpuscular volumes, thereby providing further support for the nucleotypic theoryLa teoría nucleotípica sugiere que el tamaño del genoma juega un rol indirecto en la adecuación biológica, a través de las variables con las que se relaciona. En endotermos esta teoría ha sido demostrada por la relación inversa entre la tasa metabólica basal y el tamaño del genoma. La acumulación de variables, en grupos claves de endotermos, relacionadas con esta problemática (e.g., tamaño celular son ideales para poner a prueba esta teoría. En este sentido, los picaflores son de particular interés ya que son el extremo energético dentro de los endotermos. Sabiendo que el tamaño celular es proporcional al tamaño del genoma, en este trabajo ponemos a prueba la relación del volumen corpuscular medio y la tasa metabólica basal, e indirectamente el tamaño del genoma, en cuatro especies de picaflores con masas corporales que van desde 4 a 20 g. Los datos de metabolismo mostraron estar dentro de los mayores descritos para aves, asimismo, los tamaños de los eritrocitos fueron los más pequeños dentro de los valores reportados en la literatura

  3. Caracterización del síndrome metabólico en pacientes adultos con obesidad Characterization of the metabolic syndrome in adult patients with obesity

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    Madelaine Hernández Tamayo

    2012-03-01

    Full Text Available Se realizó una caracterización clínica, validada por resultados de laboratorio, de 41 pacientes con síndrome metabólico (69,5 %, seleccionados de una muestra de 59 adultos con sobrepeso y obesidad mediante un muestreo por conglomerados y estratificado por género, detectados según los criterios del Programa Nacional de Educación sobre el Colesterol y atendidos en la Policlínica "Mario Gutiérrez" de Holguín desde noviembre de 2008 hasta junio de 2009. Los valores estadísticos se obtuvieron a través de las pruebas t de Student y Ji al cuadrado. Los hombres con síndrome metabólico tuvieron niveles más altos de tensión arterial sistólica, colesterol total y triglicéridos que los varones sin la enfermedad; las mujeres con ese estado presentaron, además, mayor peso, índice de masa corporal y circunferencia abdominal. Se comprobó una asociación entre la proteína C reactiva, la microalbuminuria y la alteración de la glucemia con el mencionado síndrome, de donde se derivó que era muy importante confirmar su presencia por el elevado riesgo que implicaba para la aparición de cardiopatía y diabetes sacarina.A clinical characterization, validated by laboratory results of 41 patients with metabolic syndrome (69,5 %, selected of a sample of 59 overweighed and obese adults was carried out by means of a sampling through conglomerates and stratified by gender, detected according to the criteria of the National Program of Education on Cholesterol and assisted in "Mario Gutiérrez" Polyclinic in Holguín from November, 2008 to June, 2009. Statistical values were obtained through the Student t and X² tests. Men with metabolic syndrome had higher levels of systolic arterial tension, total cholesterol and triglycerides than men without the disease; the women with that condition also had higher weight, body mass index and wider abdominal circumference. An association between reactive C protein, microalbuminuria and the alteration of

  4. Obesidad y factores de riesgo del síndrome metabólico en jóvenes con diabetes tipo 1

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    Mariana Prieto

    2012-08-01

    Full Text Available Los cambios en la forma de presentación de la diabetes mellitus tipo 1(DM T1 en la infancia y adolescencia asociados a la obesidad, determinaron una superposición entre los dos tipos de diabetes, con mayor heterogeneidad en su presentación clínica. Con el objetivo de caracterizar el tipo de diabetes mellitus (DM, comienzo de la diabetes, independientemente de las clasificaciones disponibles y evaluar el impacto de la obesidad en la forma de presentación, se estudiaron cincuenta niños diabéticos agrupados según su condición nutricional. Se evaluó la reserva de insulina pancreática por medición de péptido C basal, índice glucemia/péptido C y en respuesta a comida mixta (MMTT, se determinó el genotipo HLA-DQB1 y los anticuerpos GADA, ICA 512, IAA y se evaluó la presencia de factores de riesgo para enfermedad metabólica como obesidad, hipertensión, diabetes y dislipidemia en el grupo familiar. El 38% de la población presentó sobrepeso/obesidad (SP/OB. Un 82% tuvo anticuerpos positivos GADA, ICA 512, IAA sin diferencia significativa entre ambos grupos, el 100% fue positivo para genotipo HLA-DQB1 de riesgo. El 84% de los familiares presentó factores de riesgo positivo para síndrome metabólico. Se dividió a la población en dos grupos (sobrepeso/obesidad vs. normopeso. No hubo diferencias en los valores de péptido C en ayunas o en el índice glucemia/péptido C entre los dos grupos El péptido C en el MMTT mostró mayor área bajo la curva (1.77 ng/ml vs. 5.5 ng/ml, p = 0.0007 y valores más altos a los 60 y 120 minutos (p: 0.02 y 0.03 en el grupo con SP/OB. En conclusión, los pacientes con diabetes tipo 1A con SP/OB tienen mayor capacidad de respuesta pancreática medida por péptido C, sugiriendo que el estado nutricional podría actuar como acelerador del comienzo clínico de la enfermedad.

  5. Kauha : [luuletused] / Pjotr Zahharov ; udmurdi keelest tõlkinud soome keelde Viktor Shibanov, Hannu Oittinen, võru keelde tõlkinud Irina Orehhova, Kauksi Ülle, eesti keelde tõlkinud Taive Särg

    Index Scriptorium Estoniae

    Zahharov, Pjotr

    2005-01-01

    Sisu: Kauha / udmurdi keelest tõlkinud soome keelde Viktor Shibanov, Hannu Oittinen ; Kopsik / udmurdi keelest tõlkinud võru keelde Irina Orehhova, Kauksi Ülle ; Pu-pu : seitse pühendust rähnile 1, 3, 4, 4, 5-7 / udmurdi keelest tõlkinud eesti keelde Irina Orehhova, Taive Särg ; Metamorfoosid 3-8 / udmurdi keelest tõlkinud eesti keelde Irina Orehhova, Taive Särg

  6. Mapeamento do fluxo de valor do projeto executivo de arquitetura em um órgão público

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    Mariana Monteiro Xavier de Lima

    2010-05-01

    Full Text Available O presente trabalho propõe empregar a ferramenta Lean de Mapeamento do Fluxo de Valor (MFV para analisar um processo administrativo de um órgão público responsável pela elaboração de projetos voltados à habitação de interesse social. Parte-se da hipótese de que o extenso lead time do processo de elaboração de projetos é conseqüência dos desperdícios existentes. O MFV representa o fluxo de informações ao longo do tempo, o que possibilita o levantamento do tempo e dos demais recursos despendidos para a realização das atividades e a proposição de melhorias, a fim de racionalizar o processo. Este artigo foi desenvolvido a partir de um estudo exploratório, que consistiu na aplicação do MFV ao processo de elaboração do projeto executivo de arquitetura na Fundação de Desenvolvimento Habitacional da Prefeitura Municipal de Fortaleza – Habitafor. A metodologia para aplicação desta ferramenta baseou-se em propostas encontradas na literatura sobre o tema para o emprego do MFV em ambientes administrativos. Apresenta-se como resultados os mapas atual e futuro com possíveis sugestões de melhoria para o processo analisado. As alterações no processo analisado, para reduzir o lead time e garantir a estabilidade do fluxo, foram: disponibilidade online das informações relativas aos terrenos da prefeitura, inclusão no quadro de funcionários de um auxiliar administrativo e um gestor do fluxo de valor e das melhorias, emprego do safety resources, agrupamento de equipes de trabalho e padronização de procedimentos entre órgãos envolvidos no processo. Com a implantação das alterações propostas ao fluxo, estima-se uma redução de 34,2% do lead time do processo através da redução dos retrabalhos e o tempo de profissionais gastos com atividades que não agregam valor. Para que o resultado seja comprovado é necessário, em trabalhos futuros, a implementação das melhorias propostas. O estudo contribui para a

  7. A chimeric 18L1-45RG1 virus-like particle vaccine cross-protects against oncogenic alpha-7 human papillomavirus types.

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    Bettina Huber

    Full Text Available Persistent infection with oncogenic human papillomaviruses (HPV types causes all cervical and a subset of other anogenital and oropharyngeal carcinomas. Four high-risk (hr mucosal types HPV16, 18, 45, or 59 cause almost all cervical adenocarcinomas (AC, a subset of cervical cancer (CxC. Although the incidence of cervical squamous cell carcinoma (SCC has dramatically decreased following introduction of Papanicolaou (PAP screening, the proportion of AC has relatively increased. Cervical SCC arise mainly from the ectocervix, whereas AC originate primarily from the endocervical canal, which is less accessible to obtain viable PAP smears. Licensed (bivalent and quadrivalent HPV vaccines comprise virus-like particles (VLP of the most important hr HPV16 and 18, self-assembled from the major capsid protein L1. Due to mainly type-restricted efficacy, both vaccines do not target 13 additional hr mucosal types causing 30% of CxC. The papillomavirus genus alpha species 7 (α7 includes a group of hr types of which HPV18, 45, 59 are proportionally overrepresented in cervical AC and only partially (HPV18 targeted by current vaccines. To target these types, we generated a chimeric vaccine antigen that consists of a cross-neutralizing epitope (homologue of HPV16 RG1 of the L2 minor capsid protein of HPV45 genetically inserted into a surface loop of HPV18 L1 VLP (18L1-45RG1. Vaccination of NZW rabbits with 18L1-45RG1 VLP plus alum-MPL adjuvant induced high-titer neutralizing antibodies against homologous HPV18, that cross-neutralized non-cognate hr α7 types HPV39, 45, 68, but not HPV59, and low risk HPV70 in vitro, and induced a robust L1-specific cellular immune response. Passive immunization protected mice against experimental vaginal challenge with pseudovirions of HPV18, 39, 45 and 68, but not HPV59 or the distantly related α9 type HPV16. 18L1-45RG1 VLP might be combined with our previously described 16L1-16RG1 VLP to develop a second generation bivalent

  8. Os conflitos do consentimento acerca da doação de órgãos post mortem no Brasil

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    MAYNARD, Lorena Oliveira Dantas

    2015-11-01

    Full Text Available Com o refinamento das técnicas de cirurgias e imunogenética pela medicina nas últimas décadas e com os avanços na farmacoterapia, o transplante de órgãos e tecidos tornou-se uma possibilidade real e uma alternativa essencial ao prolongamento e à qualidade da vida humana. Este estudo objetivou analisar o consentimento para remoção de órgãos e tecidos post mortem na legislação brasileira, na perspectiva da autorização da família. Adotou-se, para tanto, estratégia metodológica qualitativa através de revisão de literatura e revisão legislativa, que permitem um aprofundamento no universo dos significados, dos motivos, das aspirações, das crenças, dos valores e das atitudes em relação ao tema. Os resultados apontam que, na atual Lei de Transplantes, os direitos da personalidade e o princípio da autonomia da vontade são institutos pouco homenageados em função da predominância do monopólio da decisão familiar na hipótese de um confronto entre a vontade do doador e a vontade da família. Frente ao problema da escassez de órgãos no país, a solução mais consentânea com a justiça seria uma adequação entre os dispositivos de lei do Código Civil e a Lei de Transplantes. Oportuno partir do texto já existente do artigo 4º da Lei n. 9.434/1997, para incluir a manifestação da vontade do doador em vida, devidamente documentada. É igualmente importante a informação e a conscientização popular sobre a natureza humanitária da doação de órgãos como medidas direcionadas à discussão do princípio da solidariedade para uma política de doação de órgãos.

  9. Rod consolidation of RG and E's [Rochester Gas and Electric Corporation] spent PWR [pressurized water reactor] fuel

    International Nuclear Information System (INIS)

    Bailey, W.J.

    1987-05-01

    The rod consolidation demonstration involved pulling the fuel rods from five fuel assemblies from Unit 1 of RG and E's R.E. Ginna Nuclear Power Plant. Slow and careful rod pulling efforts were used for the first and second fuel assemblies. Rod pulling then proceeded smoothly and rapidly after some minor modifications were made to the UST and D consolidation equipment. The compaction ratios attained ranged from 1.85 to 2.00 (rods with collapsed cladding were replaced by dummy rods in one fuel assembly to demonstrate the 2:1 compaction ratio capability). This demonstration involved 895 PWR fuel rods, among which there were some known defective rods (over 50 had collapsed cladding); no rods were broken or dropped during the demonstration. However, one of the rods with collapsed cladding unexplainably broke during handling operations (i.e., reconfiguration in the failed fuel canister), subsequent to the rod consolidation demonstration. The broken rod created no facility problems; the pieces were encapsulated for subsequent storage. Another broken rod was found during postdemonstration cutting operations on the nonfuel-bearing structural components from the five assemblies; evidence indicates it was broken prior to any rod consolidation operations. During the demonstration, burnish-type lines or scratches were visible on the rods that were pulled; however, experience indicates that such lines are generally produced when rods are pulled (or pushed) through the spacer grids. Rods with collapsed cladding would not enter the funnel (the transition device between the fuel assembly and the canister that aids in obtaining high compaction ratios). Reforming of the flattened areas of the cladding on those rods was attempted to make the rod cross sections more nearly circular; some of the reformed rods passed through the funnel and into the canister

  10. Electrochemical degradation of Novacron Yellow C-RG using boron-doped diamond and platinum anodes: Direct and Indirect oxidation

    International Nuclear Information System (INIS)

    Rocha, J.H. Bezerra; Gomes, M.M. Soares; Santos, E. Vieira dos; Moura, E.C. Martins de; Silva, D. Ribeiro da; Quiroz, M.A.; Martínez-Huitle, C.A.

    2014-01-01

    Graphical abstract: - Highlights: • Nature of electrode material decides the electrocatalytic mechanism followed. • Electrogenerated strong oxidants on BDD surface improve the color and organic load removal. • Chlorine active species act in solution cage oxidizing organic matter. - Abstract: The present study discusses the electrochemical degradation process of a textile dye, Novacron Yellow C-RG (NY), dissolved in synthetic wastewaters, via direct and indirect oxidation. Experiments were conducted using boron-doped diamond (BDD) and platinum supported on Ti (Pt/Ti) electrodes in the absence and presence of NaCl in the solution. The direct process for removing color is relatively similar for both anodes, while the electrochemical degradation is significantly accelerated by the presence of halogen salt in the solution. Interestingly, it does not depend on applied current density, but rather on NaCl concentration. Therefore, the electrochemical processes (direct/indirect) favor specific oxidation pathways depending on electrocatalytic material. Whereas, the Pt/Ti anode favors preferentially color removal by direct and indirect oxidation (100% of color removal) due to the fragmentation of the azo dye group; BDD electrode favors color and organic load removals in both processes (95% and up to 87%, respectively), due to the rupture of dye in different parts of its chemical structure. Parameters of removal efficiency and energy consumption for the electrochemical process were estimated. Finally, an explanation has been attempted for the role of halide, in relation with the oxygen evolution reaction, concomitant with the electrochemical incineration as well as electrocatalytic mechanisms, for each one of the electrodes used

  11. Avaliação por imagem da área de gordura visceral e suas correlações com alterações metabólicas Evaluación por imagen del área de grasa visceral y sus correlaciones con alteraciones metabólicas Imaging assessment of visceral adipose tissue area and its correlations with metabolic alterations

    Directory of Open Access Journals (Sweden)

    Anna Karla Carneiro Roriz

    2010-12-01

    Full Text Available FUNDAMENTO: A obesidade androgênica está associada a um risco maior de distúrbios metabólicos, favorecendo assim a ocorrência de doenças cardiovasculares e outras morbidades. OBJETIVO: Verificar a influência da área de tecido adiposo visceral (ATAV, medida pela tomografia computadorizada, sobre alterações metabólicas em adultos e idosos. MÉTODOS: Tomografias computadorizadas e valores de lipoproteínas: o colesterol total e frações, os triglicérides, a glicemia e o ácido úrico foram obtidos de 194 indivíduos estratificados por sexo, grupo etário e massa corporal, e analisados utilizando os testes de correlação e de média. RESULTADOS: Os idosos apresentaram maiores valores da ATAV, glicemia, ácido úrico e colesterol total. As maiores correlações foram encontradas entre a ATAV, os triglicérides e o VLDL-c (r > 0,5; p FUNDAMENTO: La obesidad androgénica está asociada a un riesgo mayor de disturbios metabólicos, favoreciendo así la ocurrencia de enfermedades cardiovasculares y otras morbilidades. OBJETIVOS: Verificar la influencia del área de tejido adiposo visceral (ATAV, medida por la tomografía computarizada, sobre alteraciones metabólicas en adultos y adultos mayores. MÉTODOS: Tomografías computarizadas y valores de lipoproteínas: el colesterol total y fracciones, los triglicéridos, la glucemia y el ácido úrico se obtuvieron de 194 individuos estratificados por sexo, grupo de edad y masa corporal, y se analizaron utilizando las pruebas de correlación y de promedio. RESULTADOS: Los adultos mayores presentaron mayores valores de la ATAV, glucemia, ácido úrico y colesterol total. Las mayores correlaciones se encontraron entre la ATAV, los triglicéridos y el VLDL-c (r > 0,5; p BACKGROUND: Androgenic obesity is associated with a higher risk of metabolic disorders, thus favoring the occurrence of cardiovascular diseases and other morbidities. OBJECTIVE: To verify the influence of the visceral adipose tissue

  12. Relationship among social support, treatment adherence and metabolic control of diabetes mellitus patients Relación entre apoyo social, adhesión al tratamiento y control metabólico de personas con Diabetes Mellitus Relação entre apoio social, adesão aos tratamentos e controle metabólico de pessoas com diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Lilian Cristiane Gomes-Villas Boas

    2012-02-01

    Full Text Available This cross-sectional and quantitative study aimed to analyze the relationship among social support, adherence to non-pharmacological (diet and physical exercise and pharmacological treatments (insulin and/or oral anti-diabetic medication and clinical and metabolic control of 162 type 2 diabetes mellitus patients. Data were collected through instruments validated for Brazil. Social support was directly correlated with treatment adherence. Adherence to non-pharmacological treatment was inversely correlated with body mass index, and medication adherence was inversely correlated with diastolic blood pressure. There were no associations between social support and clinical and metabolic control variables. Findings indicate that social support can be useful to achieve treatment adherence. Studies with other designs should be developed to broaden the analysis of relations between social support and other variables.El presente estudio objetivó analizar la relación entre apoyo social, adhesión a los tratamientos no medicamentoso (dieta y ejercicio físico y medicamentoso(insulina y/o antidiabéticos orales y control clínico-metabólico de 162 personas con diabetes mellitus tipo 2. Se trata de un estudio seccional, de abordaje cuantitativo. Los datos fueron recolectados por medio de instrumentos validados. El apoyo social tuvo correlación directa con la adhesión al tratamiento. Se observó correlación inversa entre adhesión al tratamiento no medicamentoso y índice de masa corporal, así como entre adhesión medicamentosa y presión arterial diastólica. No hubo asociaciones entre apoyo social y variables de control clínico-metabólico. Se concluye que el apoyo social podrá ser útil para obtener la adhesión a los tratamientos. Estudios con otros delineamientos deben ser desarrollados, a fin de ampliar el análisis de las relaciones entre apoyo social y otras variables.O presente estudo objetivou analisar a relação entre apoio social, ades

  13. Synergistic Use of Geniposide and Ginsenoside Rg1 Balance Microglial TNF-α and TGF-β1 following Oxygen-Glucose Deprivation In Vitro: A Genome-Wide Survey

    Directory of Open Access Journals (Sweden)

    Jun Wang

    2015-01-01

    Full Text Available Ischemia-activated microglia are like a double-edged sword, characterized by both neurotoxic and neuroprotective effects. The aim of this study was to reveal the synergistic effect of geniposide and ginsenoside Rg1 based on tumor necrosis factor- (TNF- α and transforming growth factor- (TGF- β1 balance of microglia. BV2 microglial cells were divided into 5 groups: control, model (oxygen-glucose deprivation (OGD, geniposide-treated, ginsenoside-Rg1-treated, and combination-treated. A series of assays were used to detect on (i cell viability; (ii NO content; (iii expression (content of TNF-α and TGF-β1; and (iv gene expression profiles. The results showed that integrated use of geniposide and ginsenoside Rg1 significantly inhibited NO level and protected cell viability, improved the content and expression of TGF-β1, and reduced the content and expression of TNF-α. Separated use of geniposide or ginsenoside Rg1 showed different effects at different emphases. Next-generation sequencing showed that Fcγ-receptor-mediated phagocytosis pathway played a key regulatory role in the balance of TNF-α and TGF-β1 when cotreated with geniposide and ginsenoside Rg1. These findings suggest that synergistic drug combination of geniposide and ginsenoside Rg1 in the treatment of stroke is a feasible avenue for the application.

  14. Anormalidades cardiovasculares e metabólicas em pacientes com a síndrome de Berardinelli-Seip Anormalidades cardiovasculares y metabólicas en pacientes con el síndrome de Berardinelli-Seip Cardiometabolic abnormalities in patients with berardinelli-Seip syndrome

    Directory of Open Access Journals (Sweden)

    Antonio Guedes do Rêgo

    2010-01-01

    Full Text Available FUNDAMENTO: A síndrome de Berardinelli-Seip (SBS ou lipodistrofia generalizada congênita acomete, frequentemente, o aparelho cardiovascular e também promove anormalidades metabólicas que envolvem os metabolismos glicídico e lipídico. OBJETIVO: Avaliar a prevalência das anormalidades cardiovasculares e metabólicas em portadores da SBS. MÉTODOS: Vinte e dois pacientes do Estado do Rio Grande do Norte (Brasil, com diagnóstico da SBS, foram submetidos a avaliação clínica, eletrocardiograma de repouso, ecodopplercardiograma, radiografia de tórax, eletrocardiografia dinâmica de 24 horas, teste ergométrico e análise laboratorial. RESULTADOS: Os pacientes eram, predominantemente, adultos jovens, sendo a maioria do sexo feminino. A totalidade da amostra apresentou resistência à insulina, acanthosis nigricans e HDL-colesterol diminuído. A presença de esplenomegalia, hepatomegalia, diabetes mellitus tipo II e triglicérides elevados era constante. A síndrome metabólica foi caracterizada na maioria dos pacientes, com predominância no sexo feminino e com um alto grau de consanguinidade paterna. A hipertensão arterial sistêmica e a pré-hipertensão foram encontradas em mais da metade dos pacientes (77,3%. O ecodopplercardiograma mostrou a presença de hipertrofia concêntrica do ventrículo esquerdo (50%, hipertrofia excêntrica do ventrículo esquerdo (4,5% e geometria normal do ventrículo esquerdo (45,5%. Elevada taxa de arritmia foi evidenciada no holter, tais como extrassístoles ventriculares, extrassístoles supraventriculares e taquicardia supraventricular sustentada. A incompetência cronotrópica (54,5% foi observada no teste ergométrico. CONCLUSÃO: Anormalidades cardiovasculares e metabólicas foram observadas em elevada prevalência em indivíduos jovens e assintomáticos com SBS. Esses achados apontam para a necessidade de acompanhamento cardiológico sistemático e de medidas preventivas nesse grupo de risco

  15. Alterações metabólicas e inflamatórias em condições de estresse oxidativo

    Directory of Open Access Journals (Sweden)

    José Carlos Rebuglio Vellosa

    2013-08-01

    Full Text Available O estresse oxidativo pode ser definido com o desequilíbrio entre os níveis de compostos pró-oxidantes e antioxidantes, com predomínio dos primeiros. O estado pró-oxidante vem sendo relacionado a várias patologias, como doença cardiovascular, doenças neurodegenerativas, doenças auto-imunes e cânceres, ora como sua causa, em alguns casos como consequência. Assim, torna-se importante determinar quais alterações metabólicas e inflamatórias estão relacionadas com o estresse oxidativo, para auxiliar no diagnóstico e prognóstico dessas doenças. Além dos fatores de risco clássicos, esta revisão chama a atenção para possíveis novos biomarcadores relacionados ao estresse oxidativo, entre estes estão a mieloperoxidase, paraoxonase, homocisteína e determinação do estado antioxidante.

  16. Algoritmo para el control metabólico del paciente diabético en el periodo posoperatorio de la cirugía cardiovascular

    Directory of Open Access Journals (Sweden)

    Maikel Rodulfo García

    2014-07-01

    Full Text Available El paciente con diabetes mellitus en el periodo posoperatorio de la cirugía cardiovascular, frecuentemente presenta descontrol en sus niveles de glucemia, debido a las consecuencias metabólicas que traen consigo, tanto el aumento de hormonas contra-reguladoras como la resistencia y/o disminución de los niveles de insulina, causado por el estrés quirúrgico al cual se expone el organismo. Su control es esencial durante la cirugía y luego de esta, pues de ello depende la evolución de los afectados; por tanto, es de vital importancia poseer una guía que permita orientarse para mantener las cifras de glucemia en valores adecuados. Se presenta un algoritmo terapéutico de fácil aplicación, elaborado en el Servicio de Cirugía Cardiovascular del Hospital Provincial Docente Clinicoquirúrgico "Saturnino Lora Torres" de Santiago de Cuba, con vistas a tratar la hiperglucemia, que pueda generalizarse al resto de los centros del país

  17. Comportamiento del síndrome metabólico en personas obesas que no realizan ejercicios físicos

    Directory of Open Access Journals (Sweden)

    Yudersy Izquierdo Pérez

    2007-03-01

    Full Text Available El comportamiento del síndrome metabólico en personas obesas es un trabajo que pretende ofrecer una panoràmica sobre el estudio de una enfermedad que enmascarada bajo diversas formas, anida frecuentemente en personas con tendencia al sobrepeso corporal. Se realizó un estudio descriptivo, prospectivo y de corte transversal con un grupo de pacientes que asistieron a la consulta de Dislipoproteinemia, en el período comprendido de julio del 2005 a diciembre del 2006, y que posteriormente se incorporaron a la práctica sistemática de ejercicios físicos en la pista de la FCF pinareña. En este trabajo, se describen las características generales de la muestra, como edad, sexo, antecedentes patológicos familiares y personales, hábitos tóxicos, actividad física, peso, talla y valores de glicemias, ácido úrico, fibrinógeno y lipidograma. Este estudio no llevó implícito exámenes físicos que pudieran lesionar la integridad moral de los pacientes; no obstante se obtuvo su consentimiento informado para revelar ciertos datos. En estos momentos la mayoría se encuentra sujeta a otra investigación sobre la dosis exacta de ejercicio que lleva cada uno para combatir este síndrome.

  18. Síndrome metabólica e associação com nível socioeconômico em escolares

    Directory of Open Access Journals (Sweden)

    Igor Raineh Durães Cruz

    2014-08-01

    Full Text Available Objetivo verificar a associação entre o nível socioeconômico e a presença de síndrome metabólica (SM em escolares da rede pública da cidade de Montes Claros-MG. Métodos trata-se de estudo transversal, analítico. Foram avaliados 382 escolares entre 10 e 16 anos, a partir da amostragem por conglomerados. A condição socioeconômica foi dividida em alta e baixa e a SM foi diagnosticada utilizando os critérios da International Diabetes Federation. Para análise dos dados, utilizou-se o teste qui-quadrado (p < 0,05e oddsratio (com intervalo de 95% de confiança. Resultados os escolares da classe socioeconômica baixa apresentaram alterações no estado nutricional e nos exames laboratoriais, o que contribuiu para presença da SM em 8,7% escolares. Conclusão a condição socioeconômica baixa contribui de forma significante para o diagnótico da SM e atua também na incidência dessa patologia, devido os seus pertencentes estarem mais expostos aos fatores de risco.

  19. Determinação da taxa metabólica basal em cutias, Dasyprocta azarae, por calorimetria indireta Determination of the basal metabolic rate in agoutis, Dasyprocta azarae, by indirect calorimetry

    Directory of Open Access Journals (Sweden)

    Harald F.V. Brito

    2010-06-01

    Full Text Available A melhor ferramenta para comparação fisiológica entre organismos diferentes é a taxa metabólica basal, inter-relação fundamental que existe entre todos os seres vivos. Mensurações diretas das concentrações de oxigênio e dióxido de carbono, pela análise do ar inspirado e expirado, podem ser usadas para a mensuração de taxa metabólica. Este trabalho foi executado com o propósito de aferir as taxas metabólicas basal e específica, e reexaminar o escalonamento do metabolismo basal em cutias (Dasyprocta azarae. Foram utilizadas 34 cutias (D. azarae adultas sadias, sendo 9 machos não castrados, 9 machos castrados e 16 fêmeas, pertencentes ao plantel do Criadouro Científico do Museu de História Natural Capão da Imbuia, Curitiba, PR. Os animais passaram por jejum prévio de 6 horas e foram acondicionados em caixas especiais, com temperatura ambiente controlada (22,0±1,0ºC, sendo então submetidos à aferição da taxa metabólica basal, por calorimetria indireta. Empregou-se o monitor metabólico Deltatrac®II, (Datex Ohmeda, Finlândia usualmente indicado para a mensuração da produção de dióxido de carbono (VCO2 e do consumo de oxigênio (VO2 em seres humanos, por meio da mensuração das variações na concentração de VCO2 e de VO2, com uma precisão de 0,01%. Após a aferição da taxa metabólica basal, foi calculada a taxa metabólica específica, e efetuada a análise dos dados por estatística indutiva. Os testes de hipóteses para comparação entre amostras indicaram que a taxa metabólica específica de machos não castrados é maior que a de fêmeas e machos castrados (5% de significância, e que a taxa metabólica específica de fêmeas e machos castrados é equivalente (1% de significância. Constatou-se ainda, com a análise da correlação de pontos experimentais, que outra variável que não o tamanho corporal afeta a taxa metabólica dos machos não castrados (1% de significância, o que indica a

  20. Equilíbrio acído-base e a acidose metabólica da insuficiência renal crónica

    OpenAIRE

    Andrade, Sara Isabel Oliveira de Castro e

    1999-01-01

    Resumo da tese: O organismo mantém num equilíbrio dinâmico a composição e o volume de todos os compartimentos líquidos corporais, apesar de não bebermos sempre a mesma quantidade de líquidos e de não comermos sempre os mesmos alimentos. Mantém um equilíbrio entre as entradas e as saídas e, quando percebe que algo está mal, age, por intermédio dos seus órgâos sintonizados num integrado mecanismo de regulação, sem que sequer nos apercebamos. Mas quando o organismo fica doente, quando há algo qu...

  1. Doação de órgãos: compreensão na perspectiva de adolescentes Organ donation: the opinion of adolescents

    Directory of Open Access Journals (Sweden)

    Ana Maria de Carvalho Monteiro

    2011-12-01

    Full Text Available OBJETIVOS: conhecer a perspectiva dos adolescentes sobre doação de órgãos. MÉTODOS: estudo descritivo, exploratório, de abordagem qualitativa, com 13 adolescentes entre 16 a 19 anos, de duas escolas públicas do Recife/PE. A coleta dos dados ocorreu de fevereiro a maio de 2008 com entrevistas semiestruturadas, submetidas à análise de conteúdo na modalidade temática. RESULTADOS: identificaram-se quatro temáticas: concepções que podem salvar vidas; conhecimentos revelados; sentimentos facilitadores e complicadores para a doação de órgãos; outras influências que repercutem na decisão de doar órgãos. Os adolescentes demonstraram desejo de salvar vidas doando órgãos, porém, não suficiente para uma tomada de decisão. A influência da família, mídia e amigos, surgiu como fator positivo ou de resistência, e a possibilidade de contribuir para a continuidade da vida funcionou positivamente na decisão. CONCLUSÕES: Na ótica dos adolescentes, a doação de órgãos é necessária e eles desejam contribuir, porém seus conhecimentos são inadequados. Há necessidade de informações sistemáticas e precisas para tornar realidade seu desejo em doar órgãos e salvar vidas.OBJECTIVES: to learn the opinion of adolescents regarding organ donation. METHODS: a descriptive, exploratory, qualitative study was carried out with 13 adolescents aged between 16 and 19 years at two public schools in Recife, in the Brazilian State of Pernambuco. Data were collected between February and May 2008 in semi-structured interviews that were subsequently subjected to themed content analysis. RESULTS: for themes were identified: the idea of saving lives; knowledge revealed; helpful and unhelpful feelings regarding organ donation; other issues that influence the decision to donate organs. The adolescents showed a willingness to save lives by donating organs, although this was not sufficient for them to make a decision to do so. The influence of family

  2. Morfologia de órgãos genitais femininos de quelônio semi-aquático Mesoclemmys vanderhaegei

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    Walkiria F. Silva

    Full Text Available RESUMO: O Mesoclemmys vanderhaegei (Testudines: Chelidae é um quelônio de água doce cuja ocorrência é descrita nas bacias dos rios Amazonas, Tocantins, Paraguai, Paraná e Uruguai. Consta na lista vermelha de espécies ameaçadas da International Union for Conservation of Nature, como espécie de baixo risco, mas que poderá se tornar ameaçada, sendo necessário a atualização de seus dados ecológicos e biológicos. Considerando que planos de manejo e conservação de espécies dependem também de vasto conhecimento sobre a biologia reprodutiva, apresentamos a primeira descrição macroscópica dos órgãos genitais de fêmeas jovens e adultas de M. vanderhaegei correlacionando esses achados ao tamanho do espécime e ao período do ano. Exemplares de M. vanderhaegei foram coletados no município de Chapada dos Guimarães, área de ampla ocorrência natural da espécie. A descrição dos órgãos genitais foi realizada a partir de 17 fêmeas fixadas em formol a 10% e dissecadas para evidenciação de particularidades relacionadas à anatomia externa e interna. Os órgãos genitais M. vanderhaegei jovens e adultas são constituídos por pares de ovários e ovidutos que desembocam dorsolateralmente na cloaca, formando junto com o ureter a papila urogenital. Os ovários são órgãos alongados, com a extremidade cranial mais larga e caudal afunilada, já os ovidutos são longos e localizados lateralmente aos ovários, sendo em adultas, muito diferenciado na sua forma e tamanho em relação ao das jovens. Em ambas faixas etárias, os órgãos genitais são sustentados por pregas de membrana celomática que emergem do teto da cavidade, constituindo os mesovário e mesoviduto. De acordo com a forma e o padrão de mucosa do oviduto em fêmeas adultas, o segmento cranial corresponde as regiões do infundíbulo e magnum, o segmento médio, ao istmo e no caudal identificam-se as regiões útero e vagina. No assoalho do urodeum aloja-se o clit

  3. Relación del síndrome metabólico y sus componentes con la presión del pulso en personas sin enfermedad aparente

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    Antonio J. Paragano

    2010-01-01

    Full Text Available RESUMENIntroducciónLa presión del pulso depende en gran medida de la rigidez arterial. Varios estudios se hancentrado en el hecho de que diversos factores, entre ellos el síndrome metabólico o suscomponentes, intermedian cambios que afectan en forma adversa las propiedades elásticasde las grandes arterias, acentuando su rigidez.ObjetivoEl propósito de este trabajo de investigación fue evaluar la influencia del síndrome metabólicoy sus componentes sobre la presión del pulso en personas sin enfermedad aparente.Material y métodosSe seleccionaron al azar 1.155 individuos sin enfermedad demostrable. Se registraron lasvariables que definen el síndrome metabólico (ATP III: en mg/dl y en ayunas, colesterolHDL ≤ 40/50 (hombres/mujeres, triglicéridos ≥ 150, glucemia ≥ 100, perímetro de la cintura(cm ≥ 102/88 (hombres/mujeres y presión arterial sistólica/diastólica ≥ 130/85 mm Hg.Se compararon los valores de la presión del pulso obtenidos al agrupar a los participantespor sexo y edad. Se estableció la frecuencia de los factores que definen el síndrome metabólicoy mediante regresión lineal se ajustó la presión del pulso por sexo, edad y por el conjunto deellos. A continuación se determinó el valor ajustado de la presión del pulso correspondientea cada factor del síndrome metabólico y se comparó con el de sujetos normales. Finalmente,se calculó la presión del pulso ajustada de acuerdo con las posibles combinaciones de tres omás factores (criterio diagnóstico de síndrome metabólico y se comparó con la de individuosen los que no se hallaba presente ningún componente del síndrome.ResultadosCaracterísticas generales de los 1.155 individuos: hombres 62%, edad 38 ± 9 años (rango20-66, perímetro de la cintura 89 ± 13 cm, triglicéridos 107 ± 74 mg/dl, glucemia 82 ± 16mg/dl, colesterol HDL 48 ± 13 mg/dl, presión arterial sistólica 124 ± 14 mm Hg, diastólica78 ± 9 mm Hg, presión del pulso 46 ± 9 mm Hg

  4. Preferential induction of the AhR gene battery in HepaRG cells after a single or repeated exposure to heterocyclic aromatic amines

    International Nuclear Information System (INIS)

    Dumont, Julie; Josse, Rozenn; Lambert, Carine; Antherieu, Sebastien; Laurent, Veronique; Loyer, Pascal; Robin, Marie-Anne; Guillouzo, Andre

    2010-01-01

    2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) are two of the most common heterocyclic aromatic amines (HAA) produced during cooking of meat, fish and poultry. Both HAA produce different tumor profiles in rodents and are suspected to be carcinogenic in humans. In order to better understand the molecular basis of HAA toxicity, we have analyzed gene expression profiles in the metabolically competent human HepaRG cells using pangenomic oligonucleotide microarrays, after either a single (24-h) or a repeated (28-day) exposure to 10 μM PhIP or MeIQx. The most responsive genes to both HAA were downstream targets of the arylhydrocarbon receptor (AhR): CYP1A1 and CYP1A2 after both time points and CYP1B1 and ALDH3A1 after 28 days. Accordingly, CYP1A1/1A2 induction in HAA-treated HepaRG cells was prevented by chemical inhibition or small interference RNA-mediated down-regulation of the AhR. Consistently, HAA induced activity of the CYP1A1 promoter, which contains a consensus AhR-related xenobiotic-responsive element (XRE). In addition, several other genes exhibited both time-dependent and compound-specific expression changes with, however, a smaller magnitude than previously reported for the prototypical AhR target genes. These changes concerned genes mainly related to cell growth and proliferation, apoptosis, and cancer. In conclusion, these results identify the AhR gene battery as the preferential target of PhIP and MeIQx in HepaRG cells and further support the hypothesis that intake of HAA in diet might increase human cancer risk.

  5. Human HepaRG Cells can be Cultured in Hanging-drop Plates for Cytochrome P450 Induction and Function Assays.

    Science.gov (United States)

    Murayama, Norie; Usui, Takashi; Slawny, Nicky; Chesné, Christophe; Yamazaki, Hiroshi

    2015-01-01

    Recent guidance/guidelines for industry recommend that cytochrome P450 induction can be assessed using human hepatocyte enzyme activity and/or mRNA levels to evaluate potential drug- drug interactions. To evaluate time-dependent cytochrome P450 induction precisely, induction of CYP1A2, CYP2B6, and CYP3A4 mRNA was confirmed (>2-fold) by the treatment with omeprazole, phenobarbital, and rifampicin, respectively, for 24 or 48 h on day 3 from the start of culture. After 24 h, the fold induction of CYP1A2 with 3.6 and 1.8x10(4) HepaRG cells per well was lower than that for 7.2x10(4) cells. CYP1A2 induction levels at 24 h were higher than those after 48 h. In contrast, higher CYP2B6 inductions were confirmed after 48 h exposure than after 24 h, independent of the number of cells per well. To help reduce the use of human cryopreserved hepatocytes, typical P450-dependent enzyme activities were investigated in human HepaRG cells cultured in commercial hanging-drop plates. Newly designed 96-well hanging-drop plates were capable of maintaining human CYP3A-dependent midazolam hydroxylation activities for up to 4 days using only 10% of the recommended initial 7.2x10(4) cells per well. Favorable HepaRG function using hanging-drop plates was confirmed by detecting 1'- hydroxymidazolam O-glucuronide on day 3, suggesting an improvement over traditional control plates in which this metabolite can be detected for 24-well plates. These results suggest that the catalytic function and/or induction of CYP1A2, CYP2B6, and CYP3A4 can be readily assessed with reduced numbers of starting HepaRG cells cultured in three-dimensional cultures in drops prepared with hanging-drop plates.

  6. Atitudes da equipe assistencial em relação a doação de órgãos em hospitais de Porto Alegre

    OpenAIRE

    Alessandra Rosa Vicari

    2010-01-01

    A disponibilidade e avanço nas técnicas cirúrgicas associado ao progresso na área imunológica e farmacológica (1, 2,15) tornaram o transplante de órgãos um tratamento realizado mundialmente em locais com diferenças sociais e culturais (4,5). O maior domínio no conhecimento desta terapia faz com ela possa ser oferecida atualmente para muitos pacientes. Conseqüentemente, o número de pacientes em lista de espera para transplante vem aumentando progressivamente e a realidade é uma demanda maior q...

  7. Prevalência e fisiopatologia da litíase biliar em pacientes submetidos a transplante de órgãos

    OpenAIRE

    Coelho,Júlio Cesar Uili; Contieri,Fabiana Loss de; Matias,Jorge Eduardo Fouto; Parolin,Mônica Beatriz; Godoy,Jose Luiz de

    2009-01-01

    INTRODUÇÃO: O objetivo do presente estudo é apresentar revisão da prevalência e dos principais mecanismos fisiopatológicos que levam a formação da litíase biliar em pacientes submetidos a transplante de órgãos. MÉTODOS: Revisão da literatura abrangendo 29 publicações obtidas das bases Medline/Pubmed, Scielo e Lilacs com cruzamento dos unitermos "transplante, transplante renal, transplante hepático, cálculo biliar, colecistite.". Vários estudos que utilizaram a ultrassonografia demonstraram au...

  8. Frecuencia de síndrome metabólico en residentes de una región andina del Perú

    Directory of Open Access Journals (Sweden)

    Juana Aurelia Ninatanta-Ortiz

    Full Text Available Objetivos. Estimar la frecuencia del síndrome metabólico (SM y sus características asociadas en poblaciones seleccionadas residentes de zonas urbanas de dos distritos de la región Cajamarca. Materiales y métodos. Estudio transversal realizado el 2014, en muestras aleatorias de tres poblaciones de estudio: estudiantes de secundaria, universitarios y madres de estudiantes de primaria. En adultos, el SM se definió mediante los criterios del Third Adult Treatment Panel (ATP III, en adolescentes se usó los criterios modificados de Cook. Se aplicó un cuestionario estructurado sobre prácticas saludables y hábitos nocivos. Se realizaron las estimaciones de SM para cada población de estudio, y estratificadas por sexo. Se realizó el análisis bivariado para identificar características asociadas a SM. Resultados. Enrolamos 1427 participantes (586 fueron escolares de secundaria, 305 universitarios y 536 madres de escolares de primaria. La frecuencia estimada de SM en estudiantes de secundaria fue 3,2% (IC95%: 1,7-4,8, 1,6% (IC95%: 0,5-3,8 en universitarios y 23,5% (IC95%: 19,8 - 27,2 en madres. Los componentes más prevalentes fueron bajo nivel de colesterol HDL (37,0%, 60,5% y 72,4% y la hipertrigliceridemia (46,4%, 29,9% y 38,4%, en escolares de secundaria, universitarios y madres respectivamente. Conclusiones. La frecuencia de SM fue mayor en la población de madres de estudiantes de primaria (mujeres adultas. El perfil fenotípico de SM en mujeres adultas se caracterizó por una elevada frecuencia de obesidad abdominal y dislipidemias (hipertrigliceridemia y colesterol HDL bajo mientras que en adolescentes y universitarios fueron predominantes las dislipidemias.

  9. Ácido úrico y síndrome metabólico en una población masculina de pilotos de líneas aéreas Uric acid and metabolic syndrome in aircrew members population

    Directory of Open Access Journals (Sweden)

    Juan Medina Font

    2012-03-01

    Full Text Available Introducción: Ácido úrico sérico y Síndrome Metabólico, son factores que se relacionan fuertemente con enfermedades cardiovasculares. Objetivo: conocer la prevalencia de Síndrome Metabólico así como la concentración de Ácido úrico sérico en una población aeronáutica y determinar qué posibles relaciones muestran unos niveles normales y/o patológicos de Ácido úrico con respecto a la presencia o ausencia de Síndrome Metabólico. Material y métodos: Población de 790 pilotos de líneas aéreas, todos varones. Se siguió el criterio del National Cholesterol Education Program para el diagnóstico de Síndrome Metabólico, pero modificado, utilizándose en lugar de circunferencia cintura el índice de masa corporal. Resultados: El 25,4% de los pilotos presentaron valores elevados de Ácido Úrico. El 19,9% de los pilotos presentó Síndrome Metabólico. El riesgo de presentar Síndrome Metabólico por tener el Ácido úrico elevado frente al riesgo que presentaron los que tienen el Ácido úrico normal (razón de prevalencias fue de 2,6 con un intervalo de confianza al 95% entre 2 y 3,4 y con una significación de pFundamentals: Uric acid and metabolic syndrome are strongly related to cardiovascular disease. Objective: To assess the prevalence of metabolic syndrome as well as the levels of uric acid among aircrew members and to determine the relationship between the levels of uric acid and the prevalence of metabolic syndrome. Methods: We studied a population of 790 male airline pilots. We used the definition of metabolic syndrome of the National Cholesterol Education Program Adult Treatment Panel III, but modified by replacing waist circumference for the measurement of body mass index. Results: 25.4% of the pilots presented elevated level of uric acid. 19.9% of pilots presented metabolic syndrome. The risk to present metabolic syndrome in pilots with uric acid elevated in relation to those pilots with normal uric acid was 2

  10. Prevalência da síndrome metabólica em portadoras da síndrome dos ovários policísticos Prevalence of the metabolic syndrome in women with polycystic ovary syndrome

    Directory of Open Access Journals (Sweden)

    Laura Olinda Bregieiro Fernandes Costa

    2007-01-01

    Full Text Available OBJETIVO: avaliar a prevalência da síndrome metabólica nas portadoras da síndrome dos ovários policísticos (SOP. MÉTODOS: foram avaliadas 46 mulheres com SOP, segundo os critérios de Rotterdam (2003, e 44 mulheres saudáveis, com ciclos menstruais regulares, ausência de hiperandrogenismo clínico ou laboratorial e de microcistos ovarianos ao ultra-som pélvico (grupo controle. Para o diagnóstico da síndrome metabólica foram considerados os critérios do National Cholesterol Education Program (NCEP, 2002 e da International Diabetes Federation (IDF, 2005. RESULTADOS: a prevalência da síndrome metabólica foi de 30,4% nas portadoras da SOP e de 6,8% no grupo controle, segundo critérios do NCEP, e de 32,6 e 9,1% nas pacientes com SOP e controles, respectivamente, segundo os critérios da IDF. A diferença entre estas proporções foi estatisticamente significante (p=0,004, independente do critério utilizado, do IMC e da idade. Ao analisarmos cada componente individual da síndrome metabólica, observamos que a anormalidade metabólica mais freqüente nas portadoras da SOP foi o HDL colesterol abaixo de 50 mg/dL, ocorrendo em 52,2% dos casos. As prevalências da glicemia de jejum superior a 110 mg/dL (4,3%, circunferência abdominal >88 cm (47,8%, e pressão arterial >130/85 mmHg (28,2% foram significativamente maior nas portadoras da SOP. CONCLUSÕES: a síndrome metabólica é significativamente mais freqüente nas pacientes com SOP que na população geral, independente da idade e do IMC, o que pode significar um maior risco cardiovascular nestas pacientes.PURPOSE: to evaluate the prevalence of metabolic syndrome in women with polycystic ovary syndrome (PCOS. METHODS: forty six women with PCOS, in accord with Rotterdam criteria (2003, and 44 women with regular menses, without any clinical or laboratorial hyperandrogenism features, and no ultrasonographic ovarian microcysts (control group were evaluated. For metabolic syndrome, the

  11. Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells.

    Science.gov (United States)

    Zhou, Yuanyuan; Zheng, Xia; Lu, Jiaojiao; Chen, Wei; Li, Xu; Zhao, Le

    2018-01-01

    The Warburg effect is one of the main energy metabolism features supporting cancer cell growth. 20(S)-Rg3 exerts anti-tumor effect on ovarian cancer partly by inhibiting the Warburg effect. microRNAs are important regulators of the Warburg effect. However, the microRNA regulatory network mediating the anti-Warburg effect of 20(S)-Rg3 was largely unknown. microRNA deep sequencing was performed to identify the 20(S)-Rg3-influenced microRNAs in SKOV3 ovarian cancer cells. miR-532-3p was overexpressed by mimic532-3p transfection in SKOV3 and A2780 cells or inhibited by inhibitor532-3p transfection in 20(S)-Rg3-treated cells to examine the changes in HK2 and PKM2 expression, glucose consumption, lactate production and cell growth. Dual-luciferase reporter assay was conducted to verify the direct binding of miR-532-3p to HK2. The methylation status in the promoter region of pre-miR-532-3p gene was examined by methylation-specific PCR. Expression changes of key molecules controlling DNA methylation including DNMT1, DNMT3A, DNMT3B, and TET1-3 were examined in 20(S)-Rg3-treated cells. DNMT3A was overexpressed in 20(S)-Rg3-treated cells to examine its influence on miR-532-3p level, HK2 and PKM2 expression, glucose consumption and lactate production. Deep sequencing results showed that 11 microRNAs were increased and 9 microRNAs were decreased by 20(S)-Rg3 in SKOV3 cells, which were verified by qPCR. More than 2-fold increase of miR-532-3p was found in 20(S)-Rg3-treated SKOV3 cells. Forced expression of miR-532-3p reduced HK2 and PKM2 expression, glucose consumption and lactate production in SKOV3 and A2780 ovarian cancer cells. Inhibition of miR-532-3p antagonized the suppressive effect of 20(S)-Rg3 on HK2 and PKM2 expression, glucose consumption and lactate production in ovarian cancer cells. Dual-luciferase reporter assay showed that miR-532-3p directly suppressed HK2 rather than PKM2. miR-532-3p level was controlled by the methylation in the promoter region of its host

  12. The effect of gastric dilatation in rats submitted to gasified water ingestion under the hepatic metabolic function Efeito da dilatação gástrica em ratos submetidos à ingestão de água gaseificada sobre a função metabólica hepática

    Directory of Open Access Journals (Sweden)

    José Roberto Ferreira Santiago

    2008-10-01

    xico e Brasil, nesta ordem, os três maiores paises produtores e consumidores de refrigerantes. OBJETIVO: Investigar os efeitos da dilatação gástrica em ratos submetidos a ingestão de água gaseificada, veículo uniforme para todos os refrigerantes, sobre parâmetros metabólicos da função hepática. MÉTODOS: Foram constituídos dois grupos de 15 ratos acompanhados por 15 semanas. Ao Grupo-I, foram oferecidos 200 g/dia de ração ad libitum e 100 ml de água não gaseificada em 3 períodos diários, ao Grupo-II, foram oferecidos 200 g/dia de ração ad libitum e 100 ml de água gaseificada em 3 períodos diários; em cada grupo,foram calculados a média (x e o desvio padrão (s; para todos os atributos estudados foi utilizado o método estatístico de teste t pareado, comparando-se GI com GII, testando-se o efeito dos tipos de água. RESULTADOS: Os resultados identificaram que os animais que foram submetidos ao tratamento com água gaseificada (Grupo-II, apresentaram um aumento de transaminase glutâmica pirúvica (TGP e fosfatase alcalina pp>0,05 e aumento da área gástrica com alterações morfológicas macroscópicas como o desaparecimento do pregueamento mucoso característico. CONCLUSÃO: A água gaseificada favoreceu o aumento da área gástrica com conseqüente desaparecimento macroscópico do pregueamento mucoso do órgão, que ocasionou alterações metabólicas da função hepática.

  13. Avaliação por imagem da área de gordura visceral e suas correlações com alterações metabólicas

    Directory of Open Access Journals (Sweden)

    Anna Karla Carneiro Roriz

    2010-12-01

    Full Text Available FUNDAMENTO: A obesidade androgênica está associada a um risco maior de distúrbios metabólicos, favorecendo assim a ocorrência de doenças cardiovasculares e outras morbidades. OBJETIVO: Verificar a influência da área de tecido adiposo visceral (ATAV, medida pela tomografia computadorizada, sobre alterações metabólicas em adultos e idosos. MÉTODOS: Tomografias computadorizadas e valores de lipoproteínas: o colesterol total e frações, os triglicérides, a glicemia e o ácido úrico foram obtidos de 194 indivíduos estratificados por sexo, grupo etário e massa corporal, e analisados utilizando os testes de correlação e de média. RESULTADOS: Os idosos apresentaram maiores valores da ATAV, glicemia, ácido úrico e colesterol total. As maiores correlações foram encontradas entre a ATAV, os triglicérides e o VLDL-c (r > 0,5; p < 0,01, em ambos os grupos etários. A média da área de tecido adiposo visceral mostrou-se sempre mais elevada quando os valores de triglicérides e de glicemia estavam alterados, em ambos os grupos etários. CONCLUSÃO: A maioria dos exames apresentou forte correlação com a ATAV considerada de risco para alterações metabólicas. Em idosos, a área de tecido adiposo visceral de risco parece ser superior a de adultos.

  14. Frecuencia de síndrome metabólico en adultos mayores del Distrito de San Martin de Porres de Lima, Perú según los criterios de ATP III y de la IDF

    Directory of Open Access Journals (Sweden)

    Elizabeth Aliaga

    2014-07-01

    Full Text Available Objetivos: Determinar la frecuencia del Síndrome metabólico en los adultos mayores de la comunidad del distrito de San Martín de Porres de Lima, Perú. Material y métodos: Estudio descriptivo de análisis secundario de base de datos del estudio “Perfil Clínico del Adulto Mayor que habita en la comunidad de San Martín de Porres”, el cual se realizó por muestreo probabilístico entre los meses de febrero y mayo de 2013. Resultados: Se incluyeron a 312 adultos mayores. La frecuencia del síndrome metabólico según los criterios ATP III fue 28,2%, y según los criterios de la IDF 35,3%, siendo más frecuente en mujeres que en varones. La frecuencia de obesidad según el índice de masa corporal fue 19,2%, y la frecuencia de obesidad abdominal según los criterios del ATP III fue 65,4%, siendo mayor en mujeres, mientras que según los criterios de la IDF fueron 32,1% y 59,3% en hombres y mujeres, respectivamente. Conclusiones: La frecuencia del síndrome metabólico en la población adulta mayor es alta, obteniéndose cifras mayores usando los criterios de la IDF en comparación con ATP III, presentándose con mayor frecuencia en mujeres.

  15. Síndrome metabólica em hipertensos de Cuiabá - MT: prevalência e fatores associados Síndrome metabólico en hipertensos de Cuiabá - MT: prevalencia y factores asociados Metabolic syndrome in patients with high blood pressure in Cuiabá - Mato Grosso State: prevalence and associated factors

    Directory of Open Access Journals (Sweden)

    Gilberto Paulo Pereira Franco

    2009-06-01

    Full Text Available FUNDAMENTO: A síndrome metabólica (SM é um agregado de fatores predisponentes para doenças cardiovasculares e diabete melito, cujas características epidemiológicas são insuficientemente conhecidas nos níveis regional e nacional. OBJETIVO: Estimar a prevalência de SM e fatores associados em uma amostra de hipertensos da área urbana de Cuiabá - MT. MÉTODOS: Estudo de corte transversal (maio a novembro de 2007 em amostra de 120 hipertensos (com 20 anos ou mais, pareados por gênero e selecionados por amostragem sistemática de uma população fonte de 567 hipertensos de Cuiabá. Todos os selecionados responderam a um inquérito em domicílio para obtenção de dados sócio-demográficos e hábitos de vida. Foram medidos: pressão arterial; índice de massa corpórea (IMC; circunferências da cintura e quadril; glicemia; insulinemia; lípides séricos; cálculo do índice de homeostase da resistência insulínica (HOMA; proteína C-reativa; ácido úrico e fibrinogênio. O critério para hipertensão adotado foi: média da PAS > 140mmHg e/ou PAD > 90mmHg, para síndrome metabólica segundo a I Diretriz Brasileira de Síndrome Metabólica e NCEP-ATP III. RESULTADOS: Foram analisados 120 hipertensos (60 mulheres, com média de idade de 58,3 ± 12,6 anos. Observou-se prevalência de SM de 70,8% (IC95% 61,8-78,8, com predomínio entre as mulheres (81,7% vs. 60,0%; p=0,009, sem diferenças entre adultos (71,4% e idosos (70,2%. A análise de regressão múltipla revelou uma associação positiva entre a SM e o IMC > 25 kg/m², a resistência insulínica e algum antecedente familiar de hipertensão. CONCLUSÃO: Observou-se uma elevada prevalência de SM entre hipertensos de Cuiabá, associada significativamente ao IMC >25 kg/m², à resistência insulínica (Índice HOMA e, em especial, a uma história familiar de hipertensão. Estes resultados sugerem o aprofundamento deste assunto através de novos estudos.FUNDAMENTO: El síndrome metab

  16. Mulheres com síndrome dos ovários policísticos apresentam maior frequência de síndrome metabólica independentemente do índice de massa corpóreo

    OpenAIRE

    Melo,Anderson Sanches; Macedo,Carolina Sales Vieira; Romano,Lucas Gabriel Maltoni; Ferriani,Rui Alberto; Navarro,Paula Andrea de Albuquerque Salles

    2012-01-01

    OBJETIVO: Avaliar a prevalência de síndrome metabólica e dos seus critérios definidores em mulheres com síndrome dos ovários policísticos do Sudeste brasileiro, estratificadas de acordo com o índice de massa corpóreo e comparadas com controles ovulatórias. MÉTODOS: Estudo transversal, realizado com 332 mulheres em idade reprodutiva, que foram divididas em dois grupo: Controle, constituído por 186 mulheres com ciclos menstruais regulares, sintomas ovulatórios e sem diagnóstico de síndrome dos ...

  17. Caracterização anatômica, histoquímica e de classes de metabólitos secundários de folhas de Guzmania lingulata (L. Mez.

    Directory of Open Access Journals (Sweden)

    E.M. Belotto

    2014-03-01

    Full Text Available Na medicina natural pouco se sabe sobre a importância curativa da maior parte das espécies pertencentes à Bromeliaceae. Tal fato evidencia a necessidade da realização de investigações para a comprovação de possíveis potenciais fitoterápicos em espécies dessa família. Guzmania lingulata é caracterizada como sendo uma das espécies de bromélia mais comercializada e conhecida popularmente, a qual é amplamente propagada no Brasil. O presente trabalho objetivou a caracterização anatômica e a identificação de classes de metabólitos secundários presentes em G. lingulata, por meio de análises anatômicas, histoquímicas e químicas. As folhas foram fixadas em FAA 50 e transferidas para etanol 50%. Para o estudo anatômico, secções transversais da porção mediana das lâminas foliares foram obtidas à mão livre e submetidas ao processo de dupla coloração com safranina e azul de astra, sendo, logo após, montadas em gelatina glicerinada. Para a triagem das classes de metabólitos secundários foram coletadas 10 folhas frescas que passaram pelo processo de maceração em solução hidroalcoólica 70% por sete dias, à temperatura ambiente. O filtrado resultante da filtração a vácuo foi evaporado sob vácuo a temperatura de 50ºC até a remoção total do etanol, sendo obtido desta forma o extrato bruto hidroalcoólico a frio (EBHF. Os resultados das secções histológicas permitiram a caracterização anatômico-foliar baseada na qualidade das secções manuais obtidas. Os testes em relação às classes de metabólitos secundários revelaram a presença de antocianinas e taninos e ausência de flavonóides e saponinas. Esses resultados estimulam a realização de estudos mais aprofundados para identificar e quantificar os compostos dessas e de outras classes de metabólitos.

  18. La perspectiva neuroendocrinológica para abordar el problema de la obesidad y su relación con el síndrome metabólico

    OpenAIRE

    Rocío Rodríguez Valentín; Alfonso Carreón Rodríguez

    2014-01-01

    La obesidad es considerada como la primera epidemia no infecciosa en el mundo, y por su magnitud y trascendencia, como un problema prioritario de salud global. Su asociación al síndrome metabólico, en particular a diabetes, enferme - dades cardiovasculares y cerebrales, incrementa su importancia como factor de riesgo sanitario. En este contexto, además de los análisis sociales, económicos y epidemiológicos a los que está sujeto este padecimiento, existe un amplio campo de estudio en el áre...

  19. As equações de predição da taxa metabólica basal são apropriadas para adolescentes com sobrepeso e obesidade?

    OpenAIRE

    Schneider, Patrícia; Meyer, Flávia

    2005-01-01

    As equações de predição conhecidas podem apresentar valores de taxa metabólica basal (TMB) diferentes daqueles medidos por calorimetria indireta. Os objetivos deste estudo foram descrever a TMB, por meio de calorimetria indireta, em meninos com sobrepeso e obesidade, de 12 a 17 anos de idade, residentes em Porto Alegre, Brasil, e comparar o valor medido com os valores de TMB estimados por equações de predição. A TMB foi medida por calorimetria indireta, pela manhã, em 35 voluntários, sob cond...

  20. Dieta hipocalórica e treinamento físico em pacientes com síndrome metabólica e apnéia do sono

    OpenAIRE

    Cristiane Maki Nunes

    2011-01-01

    INTRODUÇÃO. Estudos anteriores demonstraram que pacientes com síndrome metabólica (SMet) e apnéia obstrutiva do sono (SAOS) apresentam hiperativação simpática e hipersensibilidade quimiorreflexa. Neste trabalho, testamos as hipóteses de que: 1) dieta hipocalórica associada à treinamento físico (D+TF) melhora a sensibilidade quimiorreflexa em pacientes com SMet e 2) Os efeitos da D+TF seriam mais pronunciados em pacientes com SMet+AOS que em pacientes sem AOS (SMet-AOS). MÉTODOS. Vinte e sete ...

  1. Nutrigenética de la respuesta lipídica, inflamatoria y del metabolismo de la glucosa en pacientes con síndrome metabólico

    OpenAIRE

    Gómez Delgado, Francisco

    2014-01-01

    Introducción: El síndrome metabólico es una enfermedad heterogénea, caracterizada por un bajo grado de inflamación crónica en el que permanecen inciertos los mecanismos responsables de la variabilidad individual en la expresión de sus manifestaciones y en la respuesta que se obtiene tras la intervención dietética. Nuestra hipótesis es que las variaciones en determinados genes candidatos podrían interaccionar con determinados modelos de alimentación modificando la respuesta s...

  2. Avaliação de biomarcadores inflamatórios e de estresse oxidativo em adolescentes com síndrome metabólica no município de Londrina-PR

    OpenAIRE

    Kamila Landucci Bonifácio

    2014-01-01

    A síndrome metabólica (SM) é uma doença complexa com custo socioeconômico alto e é considerada mundialmente como uma epidemia. É definida como um conjunto de fatores interligados que aumentam diretamente o risco de doenças coronarianas, doença cardiovascular aterosclerótica e o diabetes mellitus tipo 2 (DM2). Desse modo, as principais alterações observadas nessa síndrome são dislipidemia, hipertensão arterial, resistência à insulina e obesidade abdominal. Alterações nos estados pró e antiinfl...

  3. Estudio de las alteraciones metabólicas inducidas por la dieta mediante técnicas de proteómica. Efecto del consumo de ácidos grasos omega-3 de origen marino

    OpenAIRE

    Méndez López, Lucía

    2016-01-01

    Las enfermedades cardiovasculares y la diabetes tipo 2 se han convertido en el principal problema de salud de los países desarrollados como consecuencia del consumo de dietas hipercalóricas y un estilo de vida sedentario. Estos problemas de salud están frecuentemente precedidos por el desarrollo de un grupo de alteraciones metabólicas que incluyen obesidad, resistencia a insulina, tolerancia a la glucosa deteriorada, dislipidemia e hipertensión, las cuales están interconectadas y caracterizan...

  4. Avaliação do perfil metabólico em indivíduos com sobrepeso e obesidade sob a suplementação oral de glutamina

    OpenAIRE

    Fabiana Tannihão

    2015-01-01

    Resumo: A obesidade é uma doença metabólica crônica caracterizada pelo excesso de gordura corporal e é considerada como um grande fator de risco para o desenvolvimento de diabetes mellitus tipo 2 (DM2). A International Diabetes Federation (IDF) estima que o Brasil tenha 13,4 milhões de pessoas (8,7% da população) com diabetes. Um aspecto fundamental na etiologia do DM2 é a resistência à insulina que possui uma grande relação com obesidade e sedentarismo. Recentes descobertas têm demonstrado q...

  5. Calidad de vida relacionada a la salud tras una intervención con dieta mediterránea y ejercicio físico en pacientes con síndrome metabólico

    OpenAIRE

    Landaeta Díaz, Leslie

    2012-01-01

    El síndrome metabólico (SMet) se asocia con depresión, estrés psicosocial y peor calidad de vida relacionada a la salud. La dieta mediterránea y el ejercicio físico tienen un efecto positivo sobre el SMet aunque se desconoce su impacto sobre la calidad de vida relacionada a la salud y la capacidad física en estos pacientes. Objetivos: Estudiar el efecto de un modelo de dieta mediterránea, combinada o no con un programa de ejercicio físico de moderada a alta intensidad, sobre...

  6. Fatores associados à síndrome metabólica em adultos atendidos em um ambulatório de nutrição = Factors associated with metabolic syndrome in adults attending a nutrition outpatient clinic

    OpenAIRE

    Hess, Simone; Tramontini, Joseane; Canuto, Raquel

    2014-01-01

    Objetivos: Estudar os fatores associados à síndrome metabólica em pacientes adultos atendidos em um ambulatório de nutrição Métodos: Um estudo transversal incluiu pacientes de 18 a 59 anos de idade, de ambos os sexos, encaminhados para atendimento no Ambulatório de Nutrição da Secretaria de Saúde da Prefeitura Municipal de Portão, estado do Rio Grande do Sul. Foram coletadas informações sociodemográficas e comportamentais através de questionários estruturados, padronizados e pré-codificado...

  7. Fatores associados à carga de doenças da síndrome metabólica entre adultos brasileiros Factors associated with the burden of metabolic syndrome diseases among Brazilian adults

    Directory of Open Access Journals (Sweden)

    Naíza Nayla Bandeira de Sá

    2010-09-01

    Full Text Available O objetivo deste trabalho é identificar fatores sociodemográficos e comportamentais associados à carga de doenças da síndrome metabólica, com base em dados coletados em 2007 pelo sistema de Vigilância de Fatores de Risco e Proteção para Doenças Crônicas por Inquérito Telefônico (VIGITEL. Foram avaliados 49.276 adultos, residentes nas capitais dos estados brasileiros e no Distrito Federal. Síndrome metabólica foi considerada na presença de pelo menos duas situações entre diabetes, dislipidemia, hipertensão arterial e obesidade. A carga de doença mostra que 22,7% da população adulta apresentam pelo menos uma das situações anteriormente descritas e 14,2% duas ou mais. As razões de prevalência ajustadas mostram que a probabilidade de síndrome metabólica é maior a partir dos 25 anos de idade, na atividade física insuficiente e na presença de excesso de peso nos dois sexos. Políticas públicas de promoção da saúde, que incorporem fatores modificáveis, que têm grande impacto na prevenção e tratamento da síndrome metabólica, devem ser estimuladas, bem como o diagnóstico clínico de populações de risco.This study aimed to identify socio-demographic and behavioral factors associated with the burden of metabolic syndrome diseases, based on data collected in 2007 by the Telephone Survey System for the Surveillance of Risk and Protective Factors for Chronic Non-Communicable Diseases (VIGITEL. The sample included 49,276 adults living in the Brazilian State capitals and Federal District. Metabolic syndrome was defined as the presence of at least two of the following: diabetes, dyslipidemia, high blood pressure, and obesity. Burden of disease showed that 22.7% of the adult population presented at least one of these conditions and 14.2% had two or more. Adjusted prevalence ratios showed that the odds of metabolic syndrome increased after 25 years of age, with insufficient physical activity, and with overweight in both

  8. Avaliação do perfil metabólico, nutricional e efeitos adversos de crianças com epilepsia refratária em uso da dieta cetogênica

    OpenAIRE

    Rizzutti,Sueli; Ramos,Ana Maria Figueiredo; Cintra,Isa de Pádua; Muszkat,Mauro; Gabbai,Alberto Alain

    2006-01-01

    OBJETIVO: Avaliar os efeitos adversos, o perfil metabólico e o crescimento pôndero-estatural de crianças com crises epilépticas de difícil controle, as quais foram submetidas a dieta cetogênica. MÉTODOS: Selecionaram-se 23 pacientes na faixa etária de 2 até 17 anos com epilepsia de difícil controle medicamentoso, sendo 43,5% (n=10) do sexo masculino e 56,5% (n=13) do sexo feminino, provenientes do Setor de Neuropediatria da Disciplina de Neurologia da Universidade Federal de São Paulo. Foram ...

  9. Estudio metabólico y cardiovascular en una cohorte de pacientes infectados por el VIH-1: predisposición genética, aterosclerosis subclínica y eventos coronarios

    OpenAIRE

    Echeverría Bermúdez, Joan Patricia

    2015-01-01

    La incidencia de la enfermedad cardiovascular (ECV) se ha incrementado en los pacientes infectados por el VIH. La replicación del VIH y la terapia antirretroviral pueden contribuir de forma independiente al aumento del riesgo de ECV. Sin embargo, la causa de este aumento del riesgo es multifactorial. Métodos: Esta tesis se centra en el estudio de los trastornos metabólicos y cardiovasculares en una cohorte de pacientes infectados por el VIH. Para ello, se llevaron a cabo tres estudios: Un pri...

  10. Efecto comparativo de una dieta rica en grasa monoinsaturada sobre la disfunción del tejido adiposo en pacientes con síndrome metabólico en estado posprandial

    OpenAIRE

    Meneses Álvarez, María Eugenia

    2013-01-01

    Introducción: Una de las características más comunes de los pacientes con síndrome metabólico (SMet) es la obesidad abdominal. El aumento y expansión que experimenta el tejido adiposo (TA) en personas obesas provoca una serie de alteraciones fisiopatológicas entre las que destaca la disfunción del TA. La exposición a un balance positivo de energía durante un largo periodo de tiempo produce fenómenos de hiperplasia (incrementa el número de adipocitos) e hipertrofia (incrementa e...

  11. Perfil metabólico y factores asociados en pacientes con esquizofrenia bajo tratamiento con antipsicóticos que acuden a consulta externa en el Instituto Nacional de Salud Mental (Perú).

    OpenAIRE

    Ricapa, Miguel A.; Guimas, Luis B.; Ticse, Ray

    2017-01-01

    Objetivo: Describir el perfil metabólico y factores asociados en pacientes con esquizofrenia bajo tratamiento con antipsicóticos que acudieron a consulta externa en el Instituto Nacional de Salud Mental “Honorio Delgado–Hideyo Noguchi” durante un periodo de tres meses. Material y métodos: Estudio transversal, realizado entre mayo y julio del 2015. De manera consecutiva, fueron incluidos pacientes con esquizofrenia, en tratamiento con el mismo esquema antipsicótico por al menos 3 meses. Se tom...

  12. Perfil metabólico y factores asociados en pacientes con esquizofrenia bajo tratamiento con antipsicóticos que acuden a consulta externa en el Instituto Nacional de Salud Mental (Perú)

    OpenAIRE

    Ricapa, Miguel A; Guimas, Luis B; Ticse, Ray

    2016-01-01

    Objetivos: Describir el perfil metabólico y factores asociados en pacientes con esquizofrenia bajo tratamiento con antipsicóticos que acudieron a consulta externa en el Instituto Nacional de Salud Mental “Honorio Delgado-Hideyo Noguchi” durante un periodo de tres meses. Material y métodos: Estudio transversal, realizado entre mayo y julio del 2015. De manera consecutiva, fueron incluidos pacientes con esquizofrenia, en tratamiento con el mismo esquema antipsicótico por al menos 3 meses. Se to...

  13. Nutrigenética y nutrigenómica: la revolución sanitaria del nuevo milenio: Implicancias clínicas en síndrome metabólico y diabetes tipo 2

    OpenAIRE

    Pisabarro, Raúl

    2006-01-01

    El creciente conocimiento de la interacción gene-nutriente revolucionará el manejo de las grandes epidemias sanitarias del siglo XXI: obesidad, síndrome metabólico y diabetes 2, cuyo piso común es el aumento de enfermedad cardiovascular y cáncer. Hoy día, mediante la nutrigenética es posible prevenir o mejorar el manejo de estas enfermedades. Esta nueva área en nutrición molecular puede dividirse en el accionar de ciertos nutrientes sobre la regulación de la expresión genética: nutrigenómica,...

  14. Carbon nanotubes as carriers of Panax ginseng metabolites and enhancers of ginsenosides Rb1 and Rg1 anti-cancer activity

    Science.gov (United States)

    Lahiani, Mohamed H.; Eassa, Souzan; Parnell, Charlette; Nima, Zeid; Ghosh, Anindya; Biris, Alexandru S.; Khodakovskaya, Mariya V.

    2017-01-01

    A major benefit to nanomaterial based-medicine is the ability to provide nanosized vehicles for sporadic metabolites. Here, we describe how the conjugation of valuable ginseng secondary metabolites (ginsenoside Rb1 or Rg1) with carbon nanotubes (CNT) can enhance their anti-proliferative and anti-cancer effects. Ginsenoside-CNT conjugate (Rb-CNT or Rg-CNT) permitted the ginsenosides to be used at a low dose, yet achieve a higher incidence of cancer killing. We were able to demonstrate that the ginsenoside-CNT conjugate can decrease cell viability up to 62% in breast cancer cells (MCF-7) and enhance antiproliferation of drug-resistant pancreatic cancer cells (PANC-1) by 61%. The interaction of the ginsenoside-CNT conjugate with breast cancer cells was studied using Raman Spectroscopy mapping. Total transcriptome profiling (Affymetrix platform) of MCF-7 cells treated with the ginsenoside-CNT conjugate shows that a number of cellular, apoptotic and response to stimulus processes were affected. Therefore, our data confirmed the potential use of CNT as a drug delivery system.

  15. Relationship Between Structure and Antiproliferative Activity of Novel 5-amino-4-cyanopyrazole-1-formaldehydehydrazono Derivatives on HL-60RG Human Leukemia Cells.

    Science.gov (United States)

    Nagahara, Yukitoshi; Nagahara, Katsuhiko

    2017-11-01

    Pyrazole derivatives have been reported to have potent antimicrobial and anticancer activity. We recently synthesized and determined the effects of analogs, benzamidoxime derivatives, on mammalian cells and discovered that benzamidoximes had an antiproliferative effect. Here we synthesized and determined the anticancer effects of hydrazonopyrazole derivatives on a mammalian cancer cell line. We synthesized 12 hydrazonopyrazole derivatives with several constant alkyl chain length or branched chains at the side chain to investigate their anticancer cell activity, using the human myelogenous leukemia cell line HL-60RG. Among all hydrazonopyrazole derivatives we synthesized, the hydrazonopyrazole derivative with a branched chain at the side chain rather than a constant alkyl chain significantly inhibited cell viability. The strongest hydrazonopyrazole derivative, 5-amino-4-cyanopyrazole-1-formaldehydehydrazono-3'-pentanal, tended to damage cells dose-dependently. This cell growth attenuation was a result of apoptosis, activating caspase-3 and fragmented DNA. Hydrazonopyrazole derivatives induced apoptosis of HL-60RG leukemia cells. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  16. Selection of reference genes for quantitative real-time RT-PCR studies in tomato fruit of the genotype MT-Rg1

    Directory of Open Access Journals (Sweden)

    Karla L. González-Aguilera

    2016-09-01

    Full Text Available Quantitative real-time RT-PCR (qRT-PCR has become one of the most widely used methods for accurate quantification of gene expression. Since there are no universal reference genes for normalization, the optimal strategy to normalize raw qRT-PCR data is to perform an initial comparison of a set of independent reference genes to assess the most stable ones in each biological model. Normalization of a qRT-PCR experiment helps to ensure that the results are both statistically significant and biologically meaningful. Tomato is the model of choice to study fleshy fruit development. The miniature tomato (Solanum lycopersicum L. cultivar Micro-Tom (MT is considered a model system for tomato genetics and functional genomics. A new genotype, containing the Rg1 allele, improves tomato in vitro regeneration. In this work, we evaluated the expression stability of four tomato reference genes, namely CAC, SAND, Expressed and ACTIN2. We showed that the genes CAC and Exp are the best reference genes of the four we tested during fruit development in the MT-Rg1 genotype. Furthermore, we validated the reference genes by showing that the expression profiles of the transcription factors FRUITFULL1 (FUL1 and APETALA2c (AP2c during fruit development are comparable to previous reports using other tomato cultivars.

  17. Perceptions Of Patients With Metabolic Syndrome Regarding Their Diet During Onset Of The Disease: A Qualitative Study [percepções De Pacientes Sobre Alimentação No Seu Processo De Adoecimento Crônico Por Síndrome Metabólica: Um Estudo Qualitativo

    OpenAIRE

    Vieira C.M.; Turato E.R.

    2010-01-01

    OBJETIVO: Investigar aspectos psicoculturais que envolvem as práticas alimentares e a imagem corporal no processo de adoecimento crônico por síndrome metabólica. MÉTODOS: Estudo clínico-qualitativo realizado por meio de entrevistas semidirigidas de questões abertas, submetidas à técnica de análise de conteúdo e interpretação dos dados com base no referencial teórico psicodinâmico. Foram entrevistados nove indivíduos em tratamento ambulatorial para síndrome metabólica em hospital universitário...

  18. Exercício resistido vs jogging em fatores de risco metabólicos de mulheres com sobrepeso/obesas Ejercicio resistido versus jogging en factores de riesgo metabólicos de mujeres con sobrepeso/obesas Circuit weight training vs jogging in metabolic risk factors of overweight/obese women

    Directory of Open Access Journals (Sweden)

    Carlos Alexandre Fett

    2009-11-01

    Full Text Available FUNDAMENTO: Exercícios resistidos e aeróbicos são recomendados para reduzir o peso e melhorar a saúde, mas ainda não foi definido qual dos dois tipos é o melhor. OBJETIVO: O objetivo do presente estudo foi comparar o exercício resistido e jogging (JOGG na doença cardiovascular múltipla (DCV, nos fatores de risco metabólicos e aptidão física de mulheres obesas ou com sobrepeso [composição corporal, perfil lipídico, acido úrico, glicose, equivalente metabólico (MET, frequência cardíaca, pressão arterial, flexibilidade, gasto de energia em repouso (GER e balanço de nitrogênio (BN]. MÉTODOS: Cinquenta mulheres foram aleatoriamente divididas em dois grupos, mas apenas 26 terminaram o estudo: exercício resistido (ER (n=14; 36±12 anos; índice de massa corporal, IMC=32±7 kg/m² e JOGG (n=12; 37±9 anos; IMC=29±2. O primeiro mês de treinamento consistiu em 60 min x 03 dias/semana e o segundo mês de treinamento consistiu em 04 dias/semana para ambos os protocolos, mais reeducação alimentar. RESULTADOS: Ambos os grupos apresentaram diminuição da massa corporal total, IMC, acido úrico plasmático e aumento do MET (pFUNDAMENTO: Ejercicios resistidos y aeróbicos se recomiendan para reducir el peso y mejorar la salud, pero todavía no se definió cuál de los dos tipos es el mejor. OBJETIVO: El objetivo del presente estudio fue comparar el ejercicio resistido y el jogging (JOGG en la enfermedad cardiovascular múltiple (ECV, en los factores de riesgo metabólicos y la aptitud física de mujeres obesas o con sobrepeso [composición corporal, perfil lipídico, ácido úrico, glucosa, equivalente metabólico (MET, frecuencia cardiaca, presión arterial, flexibilidad, gasto de energía en reposo (GER y balance de nitrógeno (BN]. MÉTODOS: Cincuenta mujeres se dividieron en dos grupos, de modo aleatorio, pero solamente 26 terminaron el estudio: ejercicio resistido (ER (n=14; 36±12 anos; índice de masa corporal, IMC=32±7 kg

  19. Influência do estado nutricional pregresso sobre o desenvolvimento da síndrome metabólica em adultos Influencia del estado nutricional previo sobre el desarrollo del síndrome metabólico en adultos Influence of prior nutritional status on the development of the metabolic syndrome in adults

    Directory of Open Access Journals (Sweden)

    Renata Maria Souza Oliveira

    2009-02-01

    Full Text Available FUNDAMENTO: O aumento da prevalência do excesso de peso em faixas etárias cada vez mais jovens pode levar ao desenvolvimento precoce de fatores de riscos cardiovasculares. OBJETIVO: Investigar a prevalência de síndrome metabólica em adultos jovens e a influência das condições de nascimento e estado nutricional na adolescência sobre este quadro. MÉTODOS: Foram avaliados cem indivíduos, em três fases da vida. A partir dos registros do banco de dados de alistamento do Tiro de Guerra, coletou-se peso, estatura e circunferência da cintura de todos os alistados nos anos de 1996, 97 e 99, para análise do estado nutricional na adolescência. A partir dos registros do mesmo banco, buscou-se informações sobre as condições de nascimento na maternidade e identificou-se os indivíduos, quando adultos. Na vida adulta, realizou-se avaliação antropométrica e bioquímica. A classificação da síndrome metabólica (SM se deu de acordo com proposta do NCEP-ATP III. O percentual de gordura corporal foi aferido por bioimpedância elétrica. A pressão arterial foi aferida por aparelho automático. A análise estatística foi realizada no software Sigma Stat 2.0, pFUNDAMENTO: El aumento de la prevalencia del exceso de peso en grupos de edad cada vez más jóvenes puede conducir al desarrollo precoz de factores de riesgo cardiovasculares. OBJETIVO: Investigar la prevalencia de síndrome metabólico en adultos jóvenes y la influencia de las condiciones de nacimiento y estado nutricional en la adolescencia sobre este cuadro. MÉTODOS: Se evaluaron cien individuos, en tres fases de la vida. A partir de los registros del banco de datos de alistamiento del Tiro de Guerra, se registraron peso, altura y circunferencia de la cintura de todos los alistados en los años de 1996, 1997 y 1999, para el análisis del estado nutricional en la adolescencia. A partir de los registros del mismo banco, se buscaron informaciones sobre las condiciones de nacimiento

  20. Pesquisa-ação com alunos do curso de graduação em enfermagem sobre o processo de doação de órgãos

    OpenAIRE

    Elton Carlos de Almeida

    2015-01-01

    Esta pesquisa centra-se na formação profissional voltada para o processo de doação/transplante de órgãos humanos, visto tratar-se de assuntos complexos que demandam, entre outros aspectos, o preparo profissional adequado para atuação oportuna. Assim, neste estudo, objetivou-se analisar a formação, o preparo e o enfrentamento dos problemas relativos ao processo de doação de órgãos, identificando esses elementos com os graduandos em Enfermagem, de uma instituição pública, possibilitando...

  1. Prevalência das alterações metabólicas em pacientes portadores de queixas vestibulares Prevalency of metabolic disorders in dizzy patients

    Directory of Open Access Journals (Sweden)

    Roseli Saraiva Moreira Bittar

    2003-01-01

    Full Text Available FORMA DE ESTUDO: Clínico prospectivo. MATERIAL E MÉTODO: Os autores avaliam a prevalência de alterações metabólicas em 325 pacientes que procuraram o Setor de Otoneurologia do Hospital das Clínicas da FMUSP com queixas vestibulares. Os pacientes foram consecutivamente atendidos entre janeiro de 1997 e janeiro de 2002. Foram avaliados os primeiros exames referentes ao protocolo clássico de investigação das tonturas, que compreende a dosagem de LDL colesterol, TSH, T3 e T4 e glicemia de jejum. As alterações encontradas foram então comparadas às observadas na população geral. As alterações mais significativas encontradas foram a elevação dos níveis de LDL colesterol, a presença de níveis alterados de hormônios tireoideanos e a maior freqüência do diabetes mellitus na população estudada. CONCLUSÃO: Conclui-se que é válida a investigação destes dados, visto que são mais freqüentes na população de risco e que a correta abordagem dessas alterações melhora o prognóstico e o controle da tontura.STUDY DESIGN: Clinical prospective. MATERIAL AND METHOD:The authors evaluated the prevalence of metabolic disorders among 325 patients with vestibular symptoms investigated by the Otoneurology Section, Otolaryngology Department of Hospital das Clínicas, Medical School, University of São Paulo. The patients were evaluated from January 1997 to January 2002, by a standard protocol which included, among other items, fast glucose levels, cholesterol, triglycerides and thyroid hormones, performed in the same laboratory. The results were compared to a standard population. Results showed higher levels of fast glucose, alteration in the thyroid hormones and also higher prevalence of diabetes in the studied population. CONCLUSION: The otolaryngologist should include the investigation of such medical problems in the routine evaluation in order to reverse them and to obtain better results in management of vestibular disorders.

  2. Prevalencia del síndrome metabólico en personas a partir de 20 años de edad. Perú, 2005

    Directory of Open Access Journals (Sweden)

    Haydeé Cárdenas Quintana

    2009-01-01

    Full Text Available Fundamento: Las enfermedades cardiovasculares constituyen las principales causas de mortalidad de la población peruana. La presencia del Síndrome Metabólico (SM se relaciona con un incremento significativo de riesgo de diabetes mellitus, enfermedad coronaria y enfermedad cerebrovascular. El presente estudio tiene por objetivo determinar la prevalencia del SM y sus componentes en la población de Perú de 20 y más años de edad. Métodos: Se estudió a 4.053 individuos representativos de la población peruana: 2.037 mujeres y 2.016 varones de 20 y más años. Se registraron datos socioeconómicos, clínicos y de laboratorio. Se extrajo sangre en ayunas para análisis bioquímicos. La condición económica de la población se caracterizó por el método de las necesidades básicas insatisfechas (NBI. Para determinar el SM se empleó el criterio planteado por la Federación Internacional de Diabetes FID. Resultados: La prevalencia de componentes del SM de la población fue: obesidad abdominal 65,6%, C-HDL disminuido 54,2%, hipertrigliceridemia 30%, hipertensión arterial 19,1% e hiperglicemias 8%. Las mujeres presentan alta prevalencia de obesidad abdominal (81%, IC95%:77,6-85,3 en comparación con los hombres (48,5%, IC95%: 44,5-52,5. La prevalencia del SM en la población fue de 25,8%, las mujeres 34,3% y los varones 16,6%, con diferencias significativas (p<0,05. Conclusiones: De los componentes del SM estudiados la obesidad abdominal fue la de mayor prevalencia en la población peruana. La prevalencia del SM en la población aumenta con la edad y disminuye a medida que se acentúa la pobreza.

  3. Resposta da taxa metabólica de repouso após 16 semanas de treinamento com pesos em mulheres na pós-menopausa

    Directory of Open Access Journals (Sweden)

    Valéria Bonganha

    2011-10-01

    Full Text Available INTRODUÇÃO: As alterações corporais provenientes da menopausa como a diminuição da massa magra (MM, aumento e redistribuição da gordura corporal e diminuição do gasto energético de repouso, colaboram para o aumento nas dimensões corporais e subsequente aumento da massa corporal total. Nesse sentido, os benefícios reconhecidos do treinamento com pesos (TP não estão atrelados apenas ao aumento da força e hipertrofia muscular, mas também à composição corporal e, consequentemente, na taxa metabólica de repouso (RMR. OBJETIVO: Avaliar a resposta da RMR após 16 semanas de TP em mulheres na pós-menopausa. MÉTODOS: Participaram 28 voluntárias, subdivididas em dois grupos: treinamento (GT n = 17 e controle (GC n = 11. O programa de TP foi realizado em três sessões semanais, em dias alternados e com duração de aproximadamente 60 min/sessão, por 16 semanas. A intensidade da carga foi determinada por meio de zona alvo de repetições máximas (RM, com reajuste semanal de carga. O consumo de oxigênio (O2 e da produção de gás carbônico (CO2, por meio de calorimetria indireta de circuito aberto, foi utilizado para cálculo da RMR segundo equação de Weir (1949. ANÁLISE ESTATÍSTICA: Foi utilizado pacote estatístico Bioestat na versão 5.0, com nível de significância de p < 0,05. RESULTADOS: Houve aumento significante dos valores de MM e força muscular, somente no GT. Não foram encontradas diferenças significantes para os valores da RMR após a intervenção para ambos os grupos. CONCLUSÃO: O programa de TP de 16 semanas foi eficiente para promover alterações na composição corporal e força muscular de mulheres na pós-menopausa; entretanto, não houve alteração da RMR após a intervenção.

  4. Alterações metabólicas e funcionais do cobre em diabetes mellitus Metabolic and functional alterations of copper in diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Lucia de Fátima Campos Pedrosa

    1999-12-01

    Full Text Available O objetivo desta revisão foi discutir aspectos que envolvem as alterações metabólicas e funcionais do cobre em Diabetes Mellitus. Na presença desta doença, alguns distúrbios funcionais de cobre têm sido caracterizados e explicados em parte por alterações nos processos de absorção, circulação e utilização do elemento. O estado hormonal pode modificar a secreção biliar de cobre e assim repercutir na regulação homeostática da absorção. A redução na atividade da lisil-oxidase, uma cuproenzima, altera a síntese de colágeno e de elastina, comprometendo assim a integridade dos vasos sangüíneos. Tal fato pode agravar o desenvolvimento de complicações vasculares nos diabéticos. Os estudos com diabetes experimental apontam um acúmulo tecidual de cobre nos rins, o que conduz à especulações quanto à gênese da nefropatia diabética. Os experimentos com pacientes diabéticos demonstram irregularidades no cobre circulante, aumento de peroxidação lipídica e estado nutricional inadequado deste micronutriente.The aim of this review was to discuss aspects that involve metabolic and functional alterations of copper in Diabetes Mellitus. In this disease some functional disturbances of copper have been explained by alterations in the processes of absorption, circulation and utilization of this element. The hormone status can modify the biliary secretion of copper and therefore to reflect on homeostatic regulation of the absorption. Impaired lysil oxidase activity (a kind of cuproenzyme alters elastin and collagen synthesis and this damages the integrity of the blood vessel. This fact can worsen the development of the vascular alterations in diabetic patients. Researches with experimental diabetes indicate high concentrations of copper in kidneys. This leads to speculations about the genesis of the diabetic nephropathy. Human studies demonstrate that diabetic patients have abnormal circulation of copper, lipid peroxidation

  5. Influencia de las hormonas metabólicas y la nutrición en el desarrollo folicular en el ganado bovino: implicaciones prácticas

    Directory of Open Access Journals (Sweden)

    Piedad Cristina Rivas López

    2011-06-01

    Full Text Available La nutrición tiene una influencia en el comportamiento reproductivo de la hembra bovina, pero el mecanismo por el cual se ve afectado dicho desempeño es poco conocido. Aunque las investigaciones se han centrado en la influencia de la nutrición sobre el eje hipotálamo-hipófisis, estudios más recientes han probado la hipótesis que las hormonas metabólicas, como señales nutricionales, ejercen un efecto directo en el ovario. En el ganado, el tratamiento con somatotropina bovina recombinante (rGH aumenta la población de pequeños folículos ováricos; esto está asociado con el aumento en las concentraciones circulantes de insulina y con el factor de crecimiento insulínico I (IGF-I. Otros estudios, tanto in vitro como in vivo, demuestran la importancia de la acción conjunta del IGF-I o la insulina con la FSH y la LH. También, se han obtenido evidencias de que al alimentar novillas supliendo el 200% de las necesidades de mantenimiento durante un corto periodo, se aumentan las concentraciones circulantes de insulina y la población de pequeños folículos ováricos. Teniendo en cuenta los últimos hallazgos, los trabajos se han encaminado a resolver algunos problemas prácticos en la reproducción del ganado de leche. En primer lugar, tanto el tratamiento previo con rGH como el aumento de la ingesta alimentaria mejoran la viabilidad embrionaria. En segundo lugar, al formular dietas destinadas a aumentar la concentración de insulina durante la lactancia temprana, se nota un pequeño aumento del consumo voluntario y que estos animales llegan más prontamente a la primera ovulación posparto, lo cual, además, muestra un aumento de la tasa de concepción al primer servicio.

  6. Uso de bicarbonato de sódio na acidose metabólica do paciente gravemente enfermo Sodium bicarbonate in the critically lll patient with metabolic acidosis

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    Paulo Novis Rocha

    2009-12-01

    Full Text Available A acidose lática é um distúrbio do equilíbrio ácido-base muito frequente em pacientes internados em unidades de terapia intensiva e está associado a um mau prognóstico. Embora exista um acúmulo substancial de evidências de que níveis críticos de acidemia provocam inúmeros efeitos adversos sobre o funcionamento celular, a utilização de bicarbonato de sódio para o tratamento da acidose lática em pacientes gravemente enfermos permanece alvo de controvérsias. Neste artigo, pretendemos: 1 analisar as principais diferenças entre as acidoses hiperclorêmicas e as acidoses orgânicas, com ânion gap (AG elevado, visando embasar a discussão sobre os fundamentos da terapia com bicarbonato de sódio nas acidoses metabólicas; 2 avaliar os riscos associados à persistência de níveis críticos de acidemia, contrastando-os com os riscos do uso de bicarbonato de sódio; 3 analisar criticamente as evidências da literatura sobre o uso de bicarbonato de sódio no tratamento da acidose lática do paciente crítico, com ênfase em ensaios clínicos randomizados em seres humanos; 4 fornecer um fundamento para a utilização judiciosa de bicarbonato de sódio nesta situação.Lactic acidosis is an acid-base imbalance frequently found in critically ill patients. It is associated with a poor prognosis. Despite the substantial body of evidence that critical levels of acidemia have several adverse effects on cell function, the use of sodium bicarbonate to treat lactic acidosis in critically ill patients remains highly controversial. This article aimed at: 1 analyzing the main differences between hyperchloremic and organic acidoses, with high anion gap; 2 comparing the risks associated with critical levels of acidemia with those associated with the use of sodium bicarbonate; 3 critically analyzing the literature evidence about the use of sodium bicarbonate for the treatment of lactic acidosis in critically ill patients, with an emphasis on randomized

  7. Terapia nutricional em pacientes portadores do HIV em uso de TARV frente ao desenvolvimento de distúrbios metabólicos induzidos pela medicação.

    Directory of Open Access Journals (Sweden)

    Paula Licks

    2016-08-01

    Full Text Available Embora a incidência de casos de pacientes portadores do vírus HIV ainda esteja aumentando, através da terapia antirretroviral (TARV é possível melhorar a qualidade de vida e diminuir os agravos provocados pela patologia.  No entanto, com a TARV observam-se efeitos colaterais importantes relativos ao metabolismo: resistência à insulina/intolerância à glicose, hipertensão arterial, lipodistrofia e dislipidemia. O objetivo do presente estudo foi revisar a literatura em busca de um maior entendimento acerca do efeito da terapia nutricional associada ou não às alterações nos hábitos de vida em pacientes portadores do HIV que fazem uso de TARV frente ao desenvolvimento de distúrbios metabólicos induzidos pela medicação. Foram revisados 11 estudos que preenchiam os critérios de inclusão, sendo que sete estudos demonstraram que a terapia nutricional apresenta impacto em alterações metabólicas, portanto uma adequada orientação nutricional deve ser parte integrante do tratamento de pacientes HIV positivos em terapia antirretroviral.

  8. O potencial da rotulação metabólica de 15N para a pesquisa de esquizofrenia The potential of 15N metabolic labeling for schizophrenia research

    Directory of Open Access Journals (Sweden)

    Michaela D. Filiou

    2013-01-01

    Full Text Available Pesquisas em psiquiatria ainda necessitam de estudos não dirigidos por hipóteses para revelar fundamentos neurobiológicos e biomarcadores moleculares para distúrbios psiquiátricos. Metodologias proteômicas disponibilizam uma série de ferramentas para esses fins. Apresentamos o princípio de rotulação metabólica utilizando 15N para proteômica quantitativa e suas aplicações em modelos animais de fenótipos psiquiátricos com um foco particular em esquizofrenia. Exploramos o potencial de rotulação metabólica por 15N em diferentes tipos de experimentos, bem como suas considerações metodológicas.Psychiatric research is in need of non-hypothesis driven approaches to unravel the neurobiological underpinnings and identify molecular biomarkers for psychiatric disorders. Proteomics methodologies constitute a state-of-the-art toolbox for biomarker discovery in psychiatric research. Here we present the principle of in vivo 15N metabolic labeling for quantitative proteomics experiments and applications of this method in animal models of psychiatric phenotypes, with a particular focus on schizophrenia. Additionally we explore the potential of 15N metabolic labeling in different experimental set-ups as well as methodological considerations of 15N metabolic labeling-based quantification studies.

  9. Papel de la testosterona y el cortisol en el síndrome metabólico y la diabetes mellitus tipo 2 Role of testosterone and cortisol in metabolic syndrome and type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Aimée M. Álvarez Álvarez

    2010-04-01

    Full Text Available INTRODUCCIÓN: el síndrome metabólico y la diabetes mellitus tipo 2 son trastornos metabólicos que han sido ampliamente abordados en la literatura científica por su alta incidencia, así como la alta morbilidad y mortalidad que a ellos se asocia. En los últimos años se han explorado nuevos elementos de posible impacto en su fisiopatogenia, dentro de los que se destacan los esteroides sexuales y los glucocorticoides. En este trabajo se revisaron y comentaron los conocimientos más actuales sobre el papel de la testosterona y el cortisol en la fisiopatogenia del síndrome metabólico y de la diabetes mellitus tipo 2 en los hombres. DESARROLLO: la testosterona desempeña un papel importante en la modulación de la sensibilidad a la insulina y en la homeostasis de la glucosa, de manera que en los hombres, los niveles bajos de testosterona resultan un elemento predictor de la diabetes mellitus tipo 2 y del síndrome metabólico. Se ha establecido la existencia de una relación bidireccional y reversible entre la deficiencia de andrógenos y la adiposidad, así como entre la deficiencia de andrógenos y la resistencia a la insulina. Se sugiere que los niveles bajos de testosterona podrían predisponer a la obesidad abdominal, que provoca una alteración del metabolismo de los ácidos grasos, lo cual a la vez promovería la resistencia a la insulina. La secreción de cortisol y la de testosterona están interrelacionadas y tienen efectos inversos sobre la resistencia a la insulina. En la obesidad abdominal el eje hipotalámico-hipofisario-adrenal se hipersensibiliza lo cual provoca aumento frecuente de la secreción de cortisol y disminución de la secreción de esteroides sexuales. Por otro lado, un aumento desproporcionado de la respuesta fisiológica al estrés, induce un incremento de la secreción de cortisol que podría a su vez causar la aparición de la resistencia a la insulina y del síndrome metabólico. Uno de los mecanismos patog

  10. Bioimpressão e produção de mini-órgãos com células tronco

    Directory of Open Access Journals (Sweden)

    Naila A. Oliveira

    Full Text Available RESUMO: A bioimpressão é considerada uma fonte promissora no desenvolvimento celular, e na produção de mini-órgãos, válulas, cartilagens que futuramente poderão ser utilizados na terapia para transplantes em animais e humanos. Assim, essa técnica poderá ser utilizada como uma terapia eletiva, no tratamento de injúrias e principalmente no tratamento de doenças crônico-degenerativas. Em humanos essa terapia está sendo pesquisada a fim de auxiliar a medicina no tratamento e regeneração de tecidos impressos a partir de arcabouços de células desenvolvidas a partir de células-tronco, biomateriais e impressões em 3D. O uso dessa tecnologia é também um auxiliar nas pesquisas oncológicas com o intuito de projetar e avaliar a proliferação celular de tumores, bem como a ação de novos medicamentos quimioterápicos. No entanto, a maior limitação para o uso da terapia utilizando-se a impressora de células, órgãos e tecidos em 3D é a falta de protocolos unificados com metodologias reprodutíveis e detalhadas; com o objetivo de viabilizar a utilização da impressora e a impressão de células, órgãos e tecidos em 3D. Dessa forma, esta revisão busca reunir as publicações mais atuais na área, as quais destacam os avanços no uso de bioimpressão com células-tronco, a fim de descrever as principais técnicas e os potenciais de utilização como alternativa terapêutica na medicina humana e veterinária.

  11. A Transcriptional Regulatory Network Containing Nuclear Receptors and Long Noncoding RNAs Controls Basal and Drug-Induced Expression of Cytochrome P450s in HepaRG Cells.

    Science.gov (United States)

    Chen, Liming; Bao, Yifan; Piekos, Stephanie C; Zhu, Kexin; Zhang, Lirong; Zhong, Xiao-Bo

    2018-07-01

    Cytochrome P450 (P450) enzymes are responsible for metabolizing drugs. Expression of P450s can directly affect drug metabolism, resulting in various outcomes in therapeutic efficacy and adverse effects. Several nuclear receptors are transcription factors that can regulate expression of P450s at both basal and drug-induced levels. Some long noncoding RNAs (lncRNAs) near a transcription factor are found to participate in the regulatory functions of the transcription factors. The aim of this study is to determine whether there is a transcriptional regulatory network containing nuclear receptors and lncRNAs controlling both basal and drug-induced expression of P450s in HepaRG cells. Small interfering RNAs or small hairpin RNAs were applied to knock down four nuclear receptors [hepatocyte nuclear factor 1 α (HNF1 α ), hepatocyte nuclear factor 4 α (HNF4 α ), pregnane X receptor (PXR), and constitutive androstane receptor (CAR)] as well as two lncRNAs [HNF1 α antisense RNA 1 (HNF1 α -AS1) and HNF4 α antisense RNA 1 (HNF4 α -AS1)] in HepaRG cells with or without treatment of phenobarbital or rifampicin. Expression of eight P450 enzymes was examined in both basal and drug-induced levels. CAR and PXR mainly regulated expression of specific P450s. HNF1 α and HNF4 α affected expression of a wide range of P450s as well as other transcription factors. HNF1 α and HNF4 α controlled the expression of their neighborhood lncRNAs, HNF1 α -AS1 and HNF4 α -AS1, respectively. HNF1 α -AS1 and HNF4 α -AS1 was also involved in the regulation of P450s and transcription factors in diverse manners. Altogether, our study concludes that a transcription regulatory network containing the nuclear receptors and lncRNAs controls both basal and drug-induced expression of P450s in HepaRG cells. Copyright © 2018 by The American Society for Pharmacology and Experimental Therapeutics.

  12. SHRIMP-RG U-Pb isotopic systematics of zircon from the Angel Lake orthogneiss, East Humboldt Range, Nevada: Is this really archean crust?

    Science.gov (United States)

    Premo, Wayne R.; Castineiras, Pedro; Wooden, Joseph L.

    2008-01-01

    New SHRIMP-RG (sensitive high-resolution ion microprobe-reverse geometry) data confirm the existence of Archean components within zircon grains of a sample from the orthogneiss of Angel Lake, Nevada, United States, previously interpreted as a nappe of Archean crust. However, the combined evidence strongly suggests that this orthogneiss is a highly deformed, Late Cretaceous monzogranite derived from melting of a sedimentary source dominated by Archean detritus. Zircon grains from the same sample used previously for isotope dilution-thermal ionization mass spectrometry (ID-TIMS) isotopic work were analyzed using the SHRIMP-RG to better define the age and origin of the orthogneiss. Prior to analysis, imaging revealed a morphological variability and intragrain, polyphase nature of the zircon population. The SHRIMP-RG yielded 207Pb/206Pb ages between ca. 2430 and 2580 Ma (a best-fit mean 207Pb/206Pb age of 2531 ± 19 Ma; 95% confidence) from mostly rounded to subrounded zircons and zircon components (cores). In addition, several analyses from rounded to subrounded cores or grains yielded discordant 207Pb/206Pb ages between ca. 1460 and ca. 2170 Ma, consistent with known regional magmatic events. All cores of Proterozoic to latest Archean age were encased within clear, typically low Th/U (206Pb/238U ages between 72 and 91 Ma, consistent with magmatic ages from Lamoille Canyon to the south. An age of ca. 90 Ma is suggested, the younger 206Pb/238U ages resulting from Pb loss. The Cretaceous and Precambrian zircon components also have distinct trace element characteristics, indicating that these age groups are not related to the same igneous source. These results support recent geophysical interpretations and negate the contention that the Archean-Proterozoic boundary extends into the central Great Basin area. They further suggest that the world-class gold deposits along the Carlin Trend are not underlain by Archean cratonal crust, but rather by the Proterozoic Mojave

  13. Eski Mezopotamya’da Siyasi Örgütlenmede Din Olgusu Religion In The Political Structure Of Ancient Mesopotamia

    Directory of Open Access Journals (Sweden)

    Yusuf KILIÇ

    2013-07-01

    zere, tarihi devirler Mezopotamya ve Mısır’da eş zamanlı olarak başlamakta, siyasi örgütlenmeler de gene buralarda karşımıza çıkmaktadır. Mezopotamya’da devletin merkezi, Kalkolitik ve Eski Tunç çağda karşımıza çıkan tapınak idi. Tapınak yalnızca dinin merkezi değil, iktisadi hayatın da merkezidir. Tapınağın bu çoğul fonksiyonu sebebi ile devletin siyasi ve idari mekanizmasının da dinden etkilenmesi, daha doğru bir ifade ile söz konusu mekanizmanın kendisine kaynak olarak dini göstermesi kaçınılmazıdır .“En” adını taşıyan kralın asli görevi, tapınağı ve ekonomiyi tanrı adına yönetmekti. Tapınak ekonomisinin en önemli vasfı, iş gücünün kontrolünü elinde tutmasıdır. Öte yandan toplumsal organizasyonların düzenli bir şekilde devam edebilmesi ve toplum içinde düzenin ve birlikteliğin sağlanması için bir takım kurallara ihtiyaç duyulmaktadır. Din, sonuçları bakımından, bu toplumsal görevi üstlenmektedir. Eskiçağ tarihi bakımından değerlendirdiğimizde iki farklı sonuç elde etmemiz mümkündür. Buna göre; “bin tanrılı halk” nitelemesinin gerisinde, dini hoşgörüyü, çeşitli halkların kendisine karşı politik bir tehlike oluşturmasını önlemek maksadıyla kullanan Hitit devleti örneğinin yanı sıra, tanrıların sayısının gittikçe azaldığı, dinin hoşgörüden ziyade merkezileştirme fonksiyonu gördüğü Sami kavimleri karşımıza çıkmaktadır. Çalışmamızın amacı, eskiçağ milletlerinin bir fetih politikası olarak din olgusunu olumlu ve olumsuz sonuçlar doğuracak yönde nasıl değerlendirdiğini gözler önüne sermektir.

  14. Importância nutricional e metabólica da fibra na dieta de equinos Nutritional and metabolic importance of fiber in the horse diet

    Directory of Open Access Journals (Sweden)

    Roberta Ariboni Brandi

    2009-07-01

    Full Text Available O equino é um herbívoro não ruminante capaz de suprir grande parte ou a totalidade da sua demanda nutricional pela ingestão de gramíneas. Apresenta a região ceco-cólica bastante desenvolvida, sendo este o principal sítio de fermentação. Este processo também ocorre na região aglandular do estômago, porém a produção de ácidos graxos voláteis é inferior, quando comparado ao intestino grosso. O conhecimento do sítio de aproveitamento de cada ingrediente é de suma importância para a combinação dos mesmos, favorecendo o ótimo aproveitamento de cada um deles e evitando excessos que podem ser prejudiciais ao metabolismo do equino. O estudo atual do fracionamento de carboidratos, por mais que não seja adaptado a fisiologia e metabolismo dos equinos, nos fornece informações sobre as diferentes frações que o compõem e com isso pode-se inferir sobre a produção de energia por cada fração gerada. A manutenção de equinos em pastejo ou sob o fornecimento de feno ou alimentos volumosos é essencial para a manutenção da atividade e saúde do seu trato digestório. O aporte mínimo de 12% de FDN garante tal situação. Atualmente, com o objetivo de aumentar a disponibilidade de energia, ingredientes como a polpa cítrica, polpa de beterraba e casca de soja, vem sendo utilizados nas formulações. Estes ingredientes de fermentação mais rápida e fácil, são uma alternativa segura para elevar a concentração energética da dieta de equinos, por proporcionar a diminuição do aporte de amido na dieta de equinos. Sabe-se entretanto, que com a elevação na qualidade do volumoso, maiores concentrações de carboidratos solúveis e de rápida fermentação são encontrados disponíveis ao longo do trato digestório dos equinos levando a alguns distúrbios metabólicos como a laminite e a sensibilidade a insulina. Com o correto manejo dos animais em pastejo e conhecimento do ciclo da planta é possível minimizar tais problemas

  15. Muscle Dysfunction in Androgen Deprivation: Role of Ryanodine Receptor

    Science.gov (United States)

    2016-11-01

    reversible pharmacological treatment is a key therapeutic goal in prostate cancer patients. This life prolonging treatment is accompanied by the adverse... reversible pharmacological treatment, is a key therapeutic goal of androgen deprivation therapies (ADT) used in patients with androgen-dependent...gel improves sexual function, mood, muscle strength, and body composition parameters in hypogonadal men. J Clin Endocrinol Metab. Aug 2000;85(8):2839

  16. Metabolic Signature of Antipsychotics used in the Treatment of Autism

    Science.gov (United States)

    2016-12-01

    pediatric, adult, and geriatric patients with schizophrenia, bipolar disorder, major depression, post-traumatic stress disorder and autism [1, 2]. While most...Endocrine manifestations of eating disorders. J.Clin.Endocrinol.Metab 96, 333-343 60. Jin,H. et al. (2008) Impact of atypical antipsychotic therapy on...2010) Olanzapine promotes fat accumulation in male rats by decreasing physical activity, repartitioning energy and increasing adipose tissue lipogenesis

  17. Pharmacological Prevention and Reversion of Erectile Dysfunction after Radical Prostatectomy, By Modulation of Nitric Oxide/Cgmp Pathways

    Science.gov (United States)

    2008-03-01

    and intensified CVOD (10); d) iNOS knock out mice had an increased collagen deposition in the penile corpora cavernosa with aging (35), and adult ...Xi L, Kukreja RC. Phosphodiesterase-5 inhibitor sildenafil preconditions adult cardiac myocytes against necrosis and apoptosis. Essential role of...in hypothyroidism . Am J Physiol Endocrinol Metab. 2007 292(1):E175-86 33. Ferrini MG, Kovanecz I, Sanchez S, Vernet D, Davila HH, Rajfer J,Gonzalez

  18. Comparison of Laboratory Experiments of Chemical, Biological, and Thermal Methods for Treatment of Chlorinated Solvent DNAPL at Kærgård Plantage in Denmark

    DEFF Research Database (Denmark)

    Christophersen, Mette; Christensen, Jørgen Fjeldsø; Jørgensen, Torben H.

    2010-01-01

    The Kærgård Plantage megasite on the western coast of Denmark represents one of the most difficult remediation challenges in Scandinavia. Disposal of an estimated 280,000 m3 of pharmaceutical wastes at the site from 1956 to 1973 resulted in the development of a complex mixture of contaminants...... in soil and groundwater, including sulfonamides, barbiturates, aniline, pyridine, chlorinated solvents (chloroethenes), fuel hydrocarbons, mercury, cyanide, lithium and many other compounds.  Wastes were disposed in six pits that continue to leach contaminants to groundwater. Contaminants in groundwater...... technologies for remediation of the residual wastes below the water table - first in the laboratory and then in pilot tests. Given the complex and highly concentrated mixture of contaminants, implementation of multiple or sequenced remediation technologies may be required to achieve cleanup goals.  An...

  19. Beobachten Sie mich! Über die Möglichkeiten von Videoüberwachung in Jörg Kalts Crash Test Dummies

    Directory of Open Access Journals (Sweden)

    Katharina StögerJörg Kalt erzeugte in seinen Filmen stets eine eigene Wirklichkeit mit eigenen Gesetzmäßigkeiten. Deshalb ist es auch nicht verwunderlich, dass die Videoüberwachung, die in seinem letzten Film Crash Test Dummies zum Einsatz kommt, ihrem eigentlichen Zweck der Kontrolle enthoben wird und als reziprokes Kommunikationsinstrument eingesetzt wird. Die Figuren lässt Kalt wie Testpersonen aufeinander prallen und zeigt die Versuche einer zwischenmenschlichen Interaktion, wenn direkte soziale Kontakte und sprachliche Kommunikation scheitern. Mit Bezug auf Kalts Biografie soll deutlich gemacht werden, wie autoritäre Systeme „gecrashed“ werden können und wie der Regisseur selbst seinen Film hacked, der sinnbildlich für einen Abschnitt der österreichischen Filmgeschichte steht.Jörg Kalt erzeugte in seinen Filmen stets eine eigene Wirklichkeit mit eigenen Gesetzmäßigkeiten. Deshalb ist es auch nicht verwunderlich, dass die Videoüberwachung, die in seinem letzten Film Crash Test Dummies zum Einsatz kommt, ihrem eigentlichen Zweck der Kontrolle enthoben wird und als reziprokes Kommunikationsinstrument eingesetzt wird. Die Figuren lässt Kalt wie Testpersonen aufeinander prallen und zeigt die Versuche einer zwischenmenschlichen Interaktion, wenn direkte soziale Kontakte und sprachliche Kommunikation scheitern. Mit Bezug auf Kalts Biografie soll deutlich gemacht werden, wie autoritäre Systeme „gecrashed“ werden können und wie der Regisseur selbst seinen Film hacked, der sinnbildlich für einen Abschnitt der österreichischen Filmgeschichte steht.

    2014-12-01

    Full Text Available Jörg Kalt erzeugte in seinen Filmen stets eine eigene Wirklichkeit mit eigenen Gesetzmäßigkeiten. Deshalb ist es auch nicht verwunderlich, dass die Videoüberwachung, die in seinem letzten Film Crash Test Dummies zum Einsatz kommt, ihrem eigentlichen Zweck der Kontrolle enthoben wird und als reziprokes Kommunikationsinstrument eingesetzt wird. Die Figuren lässt Kalt wie Testpersonen aufeinander prallen und zeigt die Versuche einer zwischenmenschlichen Interaktion, wenn direkte soziale Kontakte und sprachliche Kommunikation scheitern. Mit Bezug auf Kalts Biografie soll deutlich gemacht werden, wie autoritäre Systeme „gecrashed“ werden können und wie der Regisseur selbst seinen Film hacked, der sinnbildlich für einen Abschnitt der österreichischen Filmgeschichte steht.

  20. Comparison of EMI/RFI requirements to qualify the equipment for nuclear power plant. (RG 1.180, EPRI TR 102323, IEC 62003 and GB/T 11684)

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Tae Heon [Korea Testing Laboratory, Ansan (Korea, Republic of); Kim, Jong Seog [Research Institute of Korea Electric Power Corporation, Daejeon (Korea, Republic of); Seo, Jeong Ho; Cho, Kyoung Youn [Korea Electric Association, Seoul (Korea, Republic of)

    2011-05-15

    One issue that has been problematic for new plant equipment and especially for digital instrumentation and control (I and C) systems in recent years is electromagnetic compatibility (EMC). In some reports for nuclear power plant (NPP), electromagnetic interference (EMI), radio frequency interference (RFI), and power surges have been identified as environmental conditions that can affect the performance of safety-related electrical equipment. There are mainly two reference guides for applying to qualify EMI/RFI requirements of the equipment used in a NPP: US NRC RG 1.180 and EPRI TR 102323. Recently, IEC published the standard for the equipment in the NPP, IEC 62003. This paper has compared the requirements of these, including comparing of the requirement of the Chinese national standard, GB/T 11684

  1. Comparison of EMI/RFI requirements to qualify the equipment for nuclear power plant. (RG 1.180, EPRI TR 102323, IEC 62003 and GB/T 11684)

    International Nuclear Information System (INIS)

    Jang, Tae Heon; Kim, Jong Seog; Seo, Jeong Ho; Cho, Kyoung Youn

    2011-01-01

    One issue that has been problematic for new plant equipment and especially for digital instrumentation and control (I and C) systems in recent years is electromagnetic compatibility (EMC). In some reports for nuclear power plant (NPP), electromagnetic interference (EMI), radio frequency interference (RFI), and power surges have been identified as environmental conditions that can affect the performance of safety-related electrical equipment. There are mainly two reference guides for applying to qualify EMI/RFI requirements of the equipment used in a NPP: US NRC RG 1.180 and EPRI TR 102323. Recently, IEC published the standard for the equipment in the NPP, IEC 62003. This paper has compared the requirements of these, including comparing of the requirement of the Chinese national standard, GB/T 11684

  2. O custo que envolve a retirada de múltiplos órgãos Cost that involves the retriet of multiple organs

    Directory of Open Access Journals (Sweden)

    CÍCERA IZABEL C. DE OLIVEIRA GUERRA

    2002-06-01

    Full Text Available O processo que envolve a doação e retirada de órgãos é complexo e tem um custo elevado para os hospitais que o realizam. Neste artigo é estimado o custo médio total envolvido nesse processo, comparando-o com a remuneração paga pelo Sistema Único de Saúde. OBJETIVO: Levantar os gastos com captação, manutenção do doador e retirada de órgãos para transplante. MÉTODOS: É um estudo retrospectivo, baseado no levantamento dos prontuários de 32 doadores admitidos na Organização de Procura de Órgãos do Instituto Dante Pazzanese de Cardiologia, São Paulo, no período de janeiro a dezembro de 1999. Os gastos levantados foram em relação aos seguinte itens: recursos humanos, material de consumo, utilidade pública, exames complementares, depreciação de equipamentos, material permanente e transporte. RESULTADOS: O estudo realizado encontrou o custo médio de R$ 2.883,34 para o processo de doação, captação e retirada de órgãos, desde a avaliação até a entrega do corpo a família. O valor pago pelo SUS é de R$ 1. 853,71. Este custo cobre 65% do custo médio real. CONCLUSÃO: O artigo demonstra a importância da realização de estudos de custos, de procedimentos, com o objetivo de orientar a definição/atualização das tabelas de remuneração de serviços.The process of organ donation and retreat is complex and involves a high cost for hospitals that do it.PURPOSE: to survey the expenses with the process of donation and retreat of organs.METHODS: retrospective study based on medical records of 32 donors, admitted in the Search Organs Organisation do Instituto Dante Pazzanese de Cardiologia, during the period from January to December of 1999.RESULTS: the process is complex and involves a special structure as well as 24 hours of activities, making it costly. Expenses were related with the following items: human resources, permanent material, public utilities, complementary examinations, depreciation of equipment and

  3. Review: Jörg Strübing (2005. Pragmatistische Wissenschafts- und Technikforschung. Theorie und Methode. [Pragmatist Science and Technology Research: Theory and Method

    Directory of Open Access Journals (Sweden)

    Dirk vom Lehn

    2006-09-01

    Full Text Available There is a curious ignorance of interactionist theory and research in German sociology. Whilst Symbolic Interactionism plays a central role in courses on social theory, such courses often neglect more recent interactionist concepts and studies. Jörg STRÜBING's book introduces some of these concepts and ideas to German sociology by revealing their contribution to science and technology research and to social theory. The book explains in detail the development of interactionism and its contribution to science and technology studies. It is of interest to those studying science and technology research as well as to those interested in social theory. It should be added to reading lists of courses on science and technology studies and should contribute to the wider dissemination of interactionist theories and studies as well as to interactionist science and technology research. URN: urn:nbn:de:0114-fqs0604249

  4. Coupled Hydrodynamic and Wave Propagation Modeling for the Source Physics Experiment: Study of Rg Wave Sources for SPE and DAG series.

    Science.gov (United States)

    Larmat, C. S.; Delorey, A.; Rougier, E.; Knight, E. E.; Steedman, D. W.; Bradley, C. R.

    2017-12-01

    This presentation reports numerical modeling efforts to improve knowledge of the processes that affect seismic wave generation and propagation from underground explosions, with a focus on Rg waves. The numerical model is based on the coupling of hydrodynamic simulation codes (Abaqus, CASH and HOSS), with a 3D full waveform propagation code, SPECFEM3D. Validation datasets are provided by the Source Physics Experiment (SPE) which is a series of highly instrumented chemical explosions at the Nevada National Security Site with yields from 100kg to 5000kg. A first series of explosions in a granite emplacement has just been completed and a second series in alluvium emplacement is planned for 2018. The long-term goal of this research is to review and improve current existing seismic sources models (e.g. Mueller & Murphy, 1971; Denny & Johnson, 1991) by providing first principles calculations provided by the coupled codes capability. The hydrodynamic codes, Abaqus, CASH and HOSS, model the shocked, hydrodynamic region via equations of state for the explosive, borehole stemming and jointed/weathered granite. A new material model for unconsolidated alluvium materials has been developed and validated with past nuclear explosions, including the 10 kT 1965 Merlin event (Perret, 1971) ; Perret and Bass, 1975). We use the efficient Spectral Element Method code, SPECFEM3D (e.g. Komatitsch, 1998; 2002), and Geologic Framework Models to model the evolution of wavefield as it propagates across 3D complex structures. The coupling interface is a series of grid points of the SEM mesh situated at the edge of the hydrodynamic code domain. We will present validation tests and waveforms modeled for several SPE tests which provide evidence that the damage processes happening in the vicinity of the explosions create secondary seismic sources. These sources interfere with the original explosion moment and reduces the apparent seismic moment at the origin of Rg waves up to 20%.

  5. Eu/RG absorption and excitation spectroscopy in the solid rare gases: state dependence of crystal field splitting and Jahn-Teller coupling.

    Science.gov (United States)

    Byrne, Owen; McCaffrey, John G

    2011-03-28

    Absorption spectroscopy recorded for annealed samples of matrix-isolated atomic europium reveals a pair of thermally stable sites in Ar and Kr while a single site exists in Xe. Plots of the matrix shifts of the visible s → p bands versus host polarizability, allowed the association of the single site in Xe and the blue sites in Ar and Kr. On the basis of the similar ground state bond lengths expected for the Eu-rare gas (RG) diatomics and the known Na-RG molecules, the blue sites are attributed to Eu occupancy in the smaller tetra-vacancy while the red sites are proposed to arise from hexa-vacancy sites. Both sites are of cubic symmetry, consistent with the pronounced Jahn-Teller structure present on the y(8)P ← a(8)S(7/2) transition for these bands in the three hosts studied. Site-selective excitation spectroscopy has been used to reanalyze complex absorption spectra previously published by Jakob et al. [Phys. Lett. A 57, 67 (1976)] for the near-UV f → d transitions. On the basis that a pair of thermally stable sites exist in solid argon, the occurrence of crystal field splitting has been identified to occur for the J ≥ 5/2 level of the (8)P state when isolated in these two sites with cubic symmetry. From a detailed lineshape analysis, the magnitude of the crystal field splittings on the J = 5/2 level in Ar is found to be 105 and 123 cm(-1) for the red and blue sites, respectively.

  6. Tamanho relativo dos órgãos internos e do trato gastrintestinal de bovinos Zebu e mestiços leiteiros em sistema de recria

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    Backes Alfredo Acosta

    2006-01-01

    Full Text Available Utilizaram-se 12 bovinos machos castrados, com peso vivo médio de 257kg, sendo quatro zebu e oito mestiços leiteiros (quatro fraction one-half sangue Holandês x Gir e quatro fraction one-half sangue Holandês x Guzerá. Foram pesados e distribuídos em dois tratamentos (zebu e mestiços leiteiros, em fase de recria. Os animais foram avaliados até atingirem 340kg de peso vivo. O volumoso utilizado foi o feno de capim Tifton 85 (Cynodon dactylon. Por ocasião do abate foram coletadas amostras de rúmen, retículo, omaso, abomaso e intestinos, após o seu esvaziamento e limpeza, obtendo-se ainda os pesos e amostras de sangue, pele, pés, cabeça, fígado, rins, pulmões, língua, baço e gordura interna (mesentério e gorduras perirenal e pericardíaca. O peso do corpo vazio (PCVZ foi obtido pela soma dos pesos da carcaça, sangue, pele, pés, rabo, vísceras e órgãos. Na fase de recria e engorda, os animais zebu apresentaram a pele e o conjunto cabeça-pés-pele mais pesado do que os mestiços leiteiros. Também apresentaram os órgãos rins, baço, coração e língua mais leves do que dos mestiços. O conjunto rúmen-retículo-omaso-abomaso e os teores de gordura mesentérica e interna não apresentaram diferença entre animais mestiços leiteiros e zebu.

  7. Selecting Cells for Bioartificial Liver Devices and the Importance of a 3D Culture Environment: A Functional Comparison between the HepaRG and C3A Cell Lines.

    Science.gov (United States)

    van Wenum, Martien; Adam, Aziza A A; Hakvoort, Theodorus B M; Hendriks, Erik J; Shevchenko, Valery; van Gulik, Thomas M; Chamuleau, Robert A F M; Hoekstra, Ruurdtje

    2016-01-01

    Recently, the first clinical trials on Bioartificial Livers (BALs) loaded with a proliferative human hepatocyte cell source have started. There are two cell lines that are currently in an advanced state of BAL development; HepaRG and HepG2/C3A. In this study we aimed to compare both cell lines on applicability in BALs and to identify possible strategies for further improvement. We tested both cell lines in monolayer- and BAL cultures on growth characteristics, hepatic differentiation, nitrogen-, carbohydrate-, amino acid- and xenobiotic metabolism. Interestingly, both cell lines adapted the hepatocyte phenotype more closely when cultured in BALs; e.g. monolayer cultures produced lactate, while BAL cultures showed diminished lactate production (C3A) or conversion to elimination (HepaRG), and urea cycle activity increased upon BAL culturing in both cell lines. HepaRG-BALs outperformed C3A-BALs on xenobiotic metabolism, ammonia elimination and lactate elimination, while protein synthesis was comparable. In BAL cultures of both cell lines ammonia elimination correlated positively with glutamine production and glutamate consumption, suggesting ammonia elimination was mainly driven by the balance between glutaminase and glutamine synthetase activity. Both cell lines lacked significant urea cycle activity and both required multiple culture weeks before reaching optimal differentiation in BALs. In conclusion, culturing in BALs enhanced hepatic functionality of both cell lines and from these, the HepaRG cells are the most promising proliferative cell source for BAL application.

  8. Selecting Cells for Bioartificial Liver Devices and the Importance of a 3D Culture Environment: A Functional Comparison between the HepaRG and C3A Cell Lines

    NARCIS (Netherlands)

    van Wenum, Martien; Adam, Aziza A. A.; Hakvoort, Theodorus B. M.; Hendriks, Erik J.; Shevchenko, Valery; van Gulik, Thomas M.; Chamuleau, Robert A. F. M.; Hoekstra, Ruurdtje

    2016-01-01

    Recently, the first clinical trials on Bioartificial Livers (BALs) loaded with a proliferative human hepatocyte cell source have started. There are two cell lines that are currently in an advanced state of BAL development; HepaRG and HepG2/C3A. In this study we aimed to compare both cell lines on

  9. ORF73 LANA homologs of RRV and MneRV2 contain an extended RGG/RG-rich nuclear and nucleolar localization signal that interacts directly with importin β1 for non-classical nuclear import.

    Science.gov (United States)

    Howard, Kellie; Cherezova, Lidia; DeMaster, Laura K; Rose, Timothy M

    2017-11-01

    The latency-associated nuclear antigens (LANA) of KSHV and macaque RFHVMn, members of the RV1 rhadinovirus lineage, are closely related with conservation of complex nuclear localization signals (NLS) containing bipartite KR-rich motifs and RG-rich domains, which interact distinctly with importins α and ß1 for nuclear import via classical and non-classical pathways, respectively. RV1 LANAs are expressed in the nucleus of latently-infected cells where they inhibit replication and establish a dominant RV1 latency. Here we show that LANA homologs of macaque RRV and MneRV2 from the more distantly-related RV2 lineage, lack the KR-rich NLS, and instead have a large RG-rich NLS with multiple RG dipeptides and a conserved RGG motif. The RG-NLS interacts uniquely with importin β1, which mediates nuclear import and accumulation of RV2 LANA in the nucleolus. The alternative nuclear import and localization of RV2 LANA homologs may contribute to the dominant RV2 lytic replication phenotype. Copyright © 2017. Published by Elsevier Inc.

  10. Alterações metabólicas, terapia antirretroviral e doença cardiovascular em idosos portadores de HIV Alteraciones metabólicas, terapia antirretroviral y enfermedad cardiovascular en adultos mayores portadores de VIH Metabolic abnormalities, antiretroviral therapy and cardiovascular disease in elderly patients with HIV

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    Andréa Sebben Kramer

    2009-11-01

    Full Text Available Um dos fenômenos mais atuais da síndrome da imunodeficiência adquirida (AIDS é o surgimento de uma nova população vulnerável: os idosos. Um dos fatores responsáveis por este aumento é o desenvolvimento da terapia antirretroviral combinada (TARV, que tem proporcionado uma melhor qualidade e expectativa de vida do portador de HIV. Entretanto, a TARV está associada a efeitos adversos como dislipidemia, diabete melito e resistência à insulina, os quais se constituem como fatores de risco para doença cardiovascular. Com o impacto da TARV no metabolismo glicídico e lipídico, surgiram muitos estudos associando a infecção pelo HIV e a doença cardiovascular, assim como, os seus fatores de risco e a utilização da TARV, porém, poucos deles relatam sobre a cardiotoxicidade desta Terapia em idosos. Este artigo tem o objetivo de revisar as principais alterações metabólicas causadas pelo uso da terapia antirretroviral e o seu impacto no aumento do risco de doenças cardiovasculares nos idosos portadores de HIV.Uno de los fenómenos más actuales del síndrome de la inmunodeficiencia adquirida (SIDA es el surgimiento de una nueva población vulnerable: los adultos mayores. Uno de los factores responsables de este incremento es el desarrollo de la terapia antirretroviral combinada (TARV, que ha proporcionado una mejor calidad y expectativa de vida del portador de VIH. Sin embargo, la TARV está asociada a efectos adversos como dislipidemia, diabetes melito y resistencia a la insulina, los que se constituyen como factores de enfermedad para riesgo cardiovascular. Con el impacto de la TARV en el metabolismo glucídico y lipídico, surgieron muchos estudios asociando la infección por el riesgo y la enfermedad cardiovascular, así como, sus factores de VIH y la utilización de la TARV, sin embargo, pocos de ellos relatan sobre la cardiotoxicidad de esta terapia en adultos mayores. Este artículo tiene por objeto revisar las principales

  11. Protective Effect of Ginsenoside Rg1 on Hematopoietic Stem/Progenitor Cells through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging.

    Science.gov (United States)

    Li, Jing; Cai, Dachuan; Yao, Xin; Zhang, Yanyan; Chen, Linbo; Jing, Pengwei; Wang, Lu; Wang, Yaping

    2016-06-09

    Stem cell senescence is an important and current hypothesis accounting for organismal aging, especially the hematopoietic stem cell (HSC). Ginsenoside Rg1 is the main active pharmaceutical ingredient of ginseng, which is a traditional Chinese medicine. This study explored the protective effect of ginsenoside Rg1 on Sca-1⁺ hematopoietic stem/progenitor cells (HSC/HPCs) in a mouse model of d-galactose-induced aging. The mimetic aging mouse model was induced by continuous injection of d-gal for 42 days, and the C57BL/6 mice were respectively treated with ginsenoside Rg1, Vitamin E or normal saline after 7 days of d-gal injection. Compared with those in the d-gal administration alone group, ginsenoside Rg1 protected Sca-1⁺ HSC/HPCs by decreasing SA-β-Gal and enhancing the colony forming unit-mixture (CFU-Mix), and adjusting oxidative stress indices like reactive oxygen species (ROS), total anti-oxidant (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and malondialdehyde (MDA). In addition, ginsenoside Rg1 decreased β-catenin and c-Myc mRNA expression and enhanced the phosphorylation of GSK-3β. Moreover, ginsenoside Rg1 down-regulated advanced glycation end products (AGEs), 4-hydroxynonenal (4-HNE), phospho-histone H2A.X (r-H2A.X), 8-OHdG, p16(Ink4a), Rb, p21(Cip1/Waf1) and p53 in senescent Sca-1⁺ HSC/HPCs. Our findings indicated that ginsenoside Rg1 can improve the resistance of Sca-1⁺ HSC/HPCs in a mouse model of d-galactose-induced aging through the suppression of oxidative stress and excessive activation of the Wnt/β-catenin signaling pathway, and reduction of DNA damage response, p16(Ink4a)-Rb and p53-p21(Cip1/Waf1) signaling.

  12. Validação de equações de predição da taxa metabólica basal em mulheres residentes em Porto Alegre, RS, Brasil Validation of predictive equations of basal metabolic rate of women living in Southern Brazil

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    Vivian Wahrlich

    2001-02-01

    Full Text Available OBJETIVO: Medir a taxa metabólica basal em mulheres de 20 a 40 anos, não-gestantes ou lactantes, e comparar o valor medido com os valores de taxa metabólica basal estimados por equações de predição. MÉTODOS: A taxa metabólica basal foi medida por calorimetria indireta, pela manhã, durante a fase folicular do ciclo menstrual, em 60 voluntárias residentes no município de Porto Alegre, RS, sob condições padronizadas de jejum, repouso e ambiente. RESULTADOS: A média (± desvio-padrão da taxa metabólica basal medida foi 1.185,3±148,6 kcal em 24 horas. A taxa metabólica basal, estimada por equações, foi significativamente maior (7% a 17% do que a taxa metabólica basal medida. CONCLUSÕES: Os dados evidenciaram que as equações de predição não são adequadas para estimar a taxa metabólica basal nas mulheres avaliadas. O emprego dessas equações podem superestimar os requerimentos energéticos para mulheres com características semelhantes.OBJECTIVE: To measure the basal metabolic rate of women (aged 20 to 40 years living in Porto Alegre, Brazil, and to compare it with estimated values bored on published predictive equations. METHODS: Basal metabolic rate was measured by indirect calorimetry under standard conditions in the follicular phase of the menstrual cycle of 60 volunteers. RESULTS: Mean measured basal metabolic rate (± standard deviation was 1,185.3± 148.6 kcal/24 hours. Estimated basal metabolic rates were significantly greater (7% to 17% than measured basal metabolic rate (p<0.0001. CONCLUSIONS: These results show that predictive equations are not suitable to estimate basal metabolic rate in these groups of women and that the use of estimated basal metabolic rate will lead to an overestimation of energy requirements in women with similar characteristics.

  13. Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica Marcadores inflamatorios y anticuerpos anti-chlamydia en pacientes con síndrome metabólico Inflammatory markers and antichlamydial antibodies in patients with metabolic syndrome

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    Rosecler Riethmuller Franco

    2011-02-01

    Full Text Available FUNDAMENTO: A síndrome metabólica está associada ao aumento de risco de eventos cardiovasculares. Marcadores inflamatórios e anticorpos anti-Chlamydia têm sido relacionados ao desenvolvimento e à progressão da aterosclerose e dos eventos cardiovasculares. OBJETIVO: Avaliar os marcadores inflamatórios interleucina-6 (IL-6 e fator de necrose tumoral-alfa (TNF-α e os anticorpos anti-Chlamydia pneumoniae em pacientes com síndrome metabólica (SM, com e sem eventos cardiovasculares. MÉTODOS: Estudo transversal constituído por 147 indivíduos. Desses, 100 (68% com SM e sem eventos cardiovasculares; e 47 (32% com SM e com eventos cardiovasculares. Dos indivíduos que sofreram eventos cardiovasculares, 13 (6,11% apresentam infarto agudo do miocárdio (IAM, e dez (4,7%, acidente vascular cerebral (AVC. O diagnóstico da SM foi determinado pelos critérios do NCEP-ATPIII. RESULTADOS: A média de idade dos sujeitos com eventos cardiovasculares foi de 61,26 ± 8,5 e de 59,32 ± 9,9 nos indivíduos sem esses eventos (p=0,279, havendo predomínio do sexo feminino. O grupo com SM e sem evento apresentou maior peso, altura, IMC e circunferência abdominal. Para os indivíduos com eventos cardiovasculares (p=0,001, os marcadores inflamatórios IL-6 e TNF-α e a doença vascular periférica foram significativamente maiores. Obtiveram-se níveis elevados de anticorpos IgG para Chlamydia pneumoniae no grupo SM, sem eventos e de IgA no grupo com eventos quando comparados os dois grupos. Com relação ao IAM e ao AVC, os anticorpos anti-Chlamydia pneumoniae não demonstraram significância estatística, comparados ao grupo sem eventos cardiovasculares. Associação foi observada com o uso de estatinas, hipoglicemiantes orais, injetáveis e anti-inflamatórios não esteroidais no grupo com esses eventos. CONCLUSÃO: Marcadores inflamatórios encontram-se significativamente elevados em pacientes com SM, com IAM e AVC. Anticorpos anti-Chlamydia n

  14. Control metabólico de los adultos mayores con padecimientos de diabetes mellitus e hipertensión arterial Controle metabólico dos adultos maiores que sofrem de diabetes mellitus e hipertensáo arterial Metabolic control of elders with diabetes mellitus and hypertension

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    María Guadalupe Esmeralda Vázquez Treviño

    2011-07-01

    Full Text Available Objetivo: evaluar el comportamiento metabólico asociado a diabetes mellitus e hipertensión arterial durante la aplicación de un programa de atención para el mejoramiento de la calidad nutricional y física en el adulto mayor. Metodología: estudio de carácter comparativo; se trabajó con 20 adultos mayores seleccionados por el criterio de con­veniencia. Los datos se recabaron empleando un cuestionario estructurado que valoró los aspectos físicos y nutricionales. Al mismo tiempo se empleó un registro para llevar el control de los datos antropométricos, de hipertensión arterial, de glicemia capilar y del perfil de lípidos durante la aplicación del programa. Resultados: la población estudiada presentó una edad promedio de 69,17 años, con predominio del género femenino (95%. Durante la implementación del programa, se observa un mejoramiento notorio en cuanto a peso y glicemia capilar se re­fiere; estabilidad en lo que respecta a los niveles de colesterol (9,74% de mejoría y triglicéridos (7,47% de mejoría, mientras que los valores de hipertensión arterial y glicemia no muestran una variación significativa. También se observan cambios lige­ros en cuanto al índice de masa corporal (IMC (0,28 + 0,882, que reflejan una evolución favorable en cuanto a la calidad de vida del adulto mayor, corroborada con la prueba de Wilcoxon (0,027. Conclusión: el comportamiento metabólico del adulto mayor diabético e hipertenso reflejó un comportamiento de mejoría con la implementación de un plan de cuidados adecua­do. Dicho plan muestra un 77% de efectividad con relación a la duración del programa, que a la vez se ve reflejado en el com­portamiento gráfico de los datos.Objetivo: Avaliar o comportamiento metabólico associado á diabete e a hipertensao arterial durante a aplicação de um programa de atencao para o melhoramento da qualidade nutricional e física do adulto maior. Metodologia: Estudo comparativo em que participaram

  15. The maternal intrauterine environment as a generator of children at risk of metabolic syndrome: a review O ambiente intrauterino como fator de risco para a síndrome metabólica: uma revisão

    Directory of Open Access Journals (Sweden)

    Suzana Maria Ramos Costa

    2010-09-01

    Full Text Available Nowadays, scientists are paying special attention to the increasing prevalence of obesity and associated co-morbidities, especially metabolic syndrome. This is due to observation of the spread of this syndrome from one generation to another and the growing number of obese pregnant women, which seems to exacerbate this situation. It is not yet well established whether the pathophysiological process underlying metabolic syndrome, namely insulin resistance, is due to changes in the receptor or in the cascade of intracellular processes. This narrative review aims to report on physiological and pathological changes occurring in pregnancy and the presence of Insulin receptor, Insulin Growth Factor-I receptor and the hybrid receptor, focusing on the presence of hyperinsulinemia in the growth and development of fetuses susceptible to metabolic syndrome.O mundo científico está dando atenção especial ao crescimento da prevalência da obesidade e de suas co-morbidades, de modo particular da síndrome metabólica. Esse fato deve-se à observação da propagação dessa síndrome através de gerações e ao crescimento do número de gestantes obesas que parece agravar esta situação. Ainda não está bem estabelecido se o processo fisiopatológico subjacente à síndrome metabólica, a resistência à insulina, é por alteração no seu receptor ou na cascata de processos intracelulares. Esta revisão visa relacionar as alterações fisiológicas e patológicas da gestação e a presença dos receptores de insulina, Insulin Growth Factor-I e seus híbridos, focando na presença da hiperinsulinemia no crescimento e desenvolvimento do feto, a predisposição à síndrome metabólica.

  16. Neuroendocrine axis of stress, metabolic syndrome and psychiatric disorders in cushing's sindrome Ejes neuroendocrinos del estrés, síndrome metabólico y alteraciones psiquiátricas del síndrome de Cushing

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    Leidy Alexandra Lezcano Tobón

    2005-04-01

    Full Text Available The role of abnormalities of the hypophysishypothalamus- adrenal gland axis (HHA in the ongoing stress and depression is analyzed. Besides, it is evidenced that some problems, until recently considered merely endocrine-metabolic such as Cushing's syndrome (either clinical or subclinical and obesity (mainly when it is part of the metabolic syndrome may have a common etiological basis, be it a primary event, a comorbidity or a trigger in individuals with genetic susceptibility to states of maladaptative chronic stress. Some harmful effects of severe and/or ongoing hypercortisolism on some brain areas and the possibility of permanent alterations in some neuronal circuits are described. Finally, and according to some clinical evidences, the potential therapeutic role of antiglucocorticoids in the management of refractory depression is explored, as well as the role that early psychiatric intervention and antidepressant pharmacological treatment may play in some patients with the metabolic syndrome or increased cardiovascular risk. Se analiza el papel de las anormalidades del eje hipotálamo-hipófisis-adrenales (HHA en el estrés sostenido y la depresión. Además se pone en evidencia que algunos problemas hasta hace poco considerados puramente endocrino-metabólicos como el síndrome de Cushing (SC (clínico o subclínico y la obesidad, principalmente cuando hace parte del síndrome metabólico (SM, pueden tener una base etiológica común, como evento primario, comorbilidad o disparador en individuos con susceptibilidad genética a los estados de estrés crónico maladaptativo. Se describen algunas acciones lesivas del hipercortisolismo severo y/o sostenido en algunas áreas cerebrales y la posibilidad de producir alteraciones permanentes en algunos circuitos neuronales. Por último se explora, de acuerdo con algunas evidencias clínicas, el papel terapéutico potencial de fármacos antiglucocorticoides en el manejo de la depresión refractaria y

  17. Repercussão da perda de peso sobre parâmetros nutricionais e metabólicos de pacientes obesos graves após um ano de gastroplastia em Y-de-Roux

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    Luziane Della Costa

    Full Text Available OBJETIVO: Avaliar a evolução metabólico-nutricional e a atividade inflamatória em pacientes com obesidade grave submetidos à cirurgia bariátrica. MÉTODOS: Realizou-se um estudo prospectivo em 56 pacientes (50 mulheres e seis homens, apresentando média de idade de 40 +/- 9,9 anos, submetidos à RYGB. Avaliação metabólica e nutricional e da atividade inflamatória foram verificadas antes, seis e 12 meses após o procedimento cirúrgico. RESULTADOS: Verificou-se redução significativa nos valores iniciais, em relação à perda de peso de 138 ± 28,8 to 90 ± 19,5 kg (p< 0,0001, glicemia de 116 ± 47,3 to 84 ± 9,8 mg/dL (p< 0,0001, níveis de triacilglicerol de 137 ± 61,4 to 84 ± 38,6 mg/dL (p< 0,0001, colesterol total de 189 ± 41,6 to 166 ± 36,4 mg/dL (p< 0,0001 e LDL-colesterol de 119 ± 36,1 para 104 ± 30,7 mg/dL (p< 0,0005. Os níveis de proteína C-reativa reduziram de 11,33 ± 10,82 para 3,62 ± 4,49 mg/dL (p< 0,0001. Embora os níveis de ferro tenham permanecido dentro do limite de normalidade, após um ano, observou-se diminuição significativa na hemoglobina de 13 ± 1,3 para 12 ± 1,4 g/dL (p< 0,01, e redução nos níveis de ferritina, particularmente nas mulheres, que apresentou queda de 101,2 ± 123,3 para 85,0 ± 101,9 g/dL (p< 0,03. CONCLUSÃO: A melhora verificada no estado metabólico e inflamatório concomitantemente após tratamento cirúrgico pode reduzir substancialmente as co-morbidades associadas com o risco cardiovascular aumentado.

  18. Auto-anticorpos anti-β2-glicoproteína I e síndrome metabólica

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    Rodrigo B. Krás Borges

    2011-04-01

    Full Text Available FUNDAMENTO: A síndrome metabólica (SM é uma entidade pró-aterogênica. Autoanticorpos tais como β2-glicoproteína I (β2-gpI podem influenciar o aparecimento de ateromas. Estudos anteriores confirmaram uma associação entre anticorpos IgA anti-β2-gpI e isquemia cerebral, infarto do miocárdio, doença arterial periférica e doença da carótida. OBJETIVO: O objetivo desse estudo de caso-controle foi avaliar uma possível associação entre anticorpos anti-β2-gpI e anticardiolipina (aCL com SM não-complicada. MÉTODOS: Pacientes com SM sem histórico de eventos vasculares e indivíduos-controle, consistindo em pacientes da Enfermaria de Ortopedia admitidos devido a doenças musculoesqueléticas foram incluídos no estudo. Idade, sexo, etnia, histórico de hipertensão, tabagismo, hipercolesterolemia e diabetes mellitus foram avaliados como fatores de risco em ambos os grupos. Anticorpos IgG, IgM, e IgA anti-β2-gpI e aCL foram detectados através de imunoensaios enzimáticos. RESULTADOS: Um total de 68 pacientes com SM e 82 controles foram estudados. Os pacientes com SM tinham média de idade superior à dos controles (P = 0,001, enquanto homens (P = 0,003; OR 0,31; IC95%: 0,15-0,16 e etnia caucasiana (P = 0,004; OR 0,25; IC95%:0,10-0,60 eram predominantes nos controles. Histórico de hipertensão, hipercolesterolemia e diabetes mellitus foi mais prevalente nos pacientes com SM do que nos controles (P < 0.05. A frequência de anticorpos aCL (todos os isotipos e do IgG e IgM anti-β2 gpI não diferiu de forma significante nos pacientes com SM e controles. Anticorpos IgA anti-β2-gpI foram significantemente mais frequentes nos pacientes com SM (42,2% do que nos controles (10,9% (P < 0,001. O OR ajustado para anticorpos IgA anti-β2-gpI foi 3,60 (IC95%: 1,55-8,37; P = 0,003. CONCLUSÃO: O presente estudo mostra que níveis elevados de autoanticorpos IgA para β2-gpI podem estar independentemente associados com SM.

  19. Níveis de atividade física e alterações metabólicas em pessoas vivendo com HIV/AIDS

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    Jayder Lucas Hotts Romancini

    2012-12-01

    Full Text Available Com a introdução da highly active antiretroviral therapy (HAART - terapia antirretroviral fortemente ativa - o curso da infecção pelo HIV sofreu profundas modificações; ocorreu aumento da sobrevida e melhora na qualidade de vida, com restauração parcial do sistema imune. Após cerca de 10 anos do uso da HAART, começaram a aparecer os efeitos da combinação tratamento/vírus. Estes incluem alterações no metabolismo dos lipídios com hipertrigliceridemia, hipercolesterolemia, resistência insulínica, hiperglicemia e redistribuição da gordura corporal que são fatores de risco para doença cardiovascular. Estudos observacionais em pessoas vivendo com HIV/AIDS mostram que o indivíduo, ao se descobrir portador da doença, acaba sentindo medo ou vergonha, reduzindo o círculo social, atividades de trabalho e lazer, e se isolando em casa ou em pequenos grupos os quais tenham em comum a identidade de portadores HIV/AIDS. O objetivo deste trabalho foi avaliar a relação entre o nível de atividade física habitual e de lazer de pessoas vivendo com HIV/AIDS e sua relação com alterações metabólicas. Os pacientes foram classificados em sedentários e ativos, utilizando o questionário de atividade física habitual proposto por Baecke e validado para pacientes HIV/AIDS. Para classificação utilizou-se as recomendações do Colégio Americano de Medicina do Esporte. O metabolismo foi avaliado por meio das dosagens de glicemia de jejum, colesterol total, HDL-colesterol, LDL-colesterol e triglicérides, utilizando-se kits comerciais. A amostra foi composta por 65 pacientes HIV que utilizavam terapia HAART há pelo menos cinco anos. Foram classificados 64,6% como sedentários e 35,4% ativos. O estudo mostra uma relação direta entre nível de atividade física e melhores níveis de HDL-colesterol em pacientes HIV/AIDS. O HDL-colesterol é uma lipoproteína de proteção cardiovascular e, embora outros parâmetros avaliados não tenham

  20. Metabolic syndrome in obese adolescents: what is enough? Síndrome metabólica em adolescentes obesos: o que é suficiente?

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    Leticia Esposito Sewaybricker

    2013-02-01

    Full Text Available OBJECTIVE: To study the agreement among three distinct criteria for metabolic syndrome (MS adapted to adolescents, and to identify associated factors for MS. METHODS: Cross-sectional study with 65 obese subjects aged 10 to 18 years, attended to at the Outpatient Clinic for Obese Children and Adolescents at the Clinical Hospital of the Universidade Estadual de Campinas (Unicamp. MS was defined using the criteria of the World Health Organization (WHO, the International Diabetes Federation (IDF, and the Adult Treatment Panel III (ATP III. Clinical, anthropometrical, and laboratorial data were associated to MS. RESULTS: From the 65 subjects, none had MS according to the WHO criteria, while 18 were diagnosed with MS (27.6% according to the IDF, and 19 (29.2% according to the ATP III. Agreement between IDF and ATP III was excellent (kappa 81%. In this study, puberty and triglycerides levels showed significant statistical difference when comparing subjects with and without MS, the first for ATP III (p = 0.03, and the second for IDF (p = 0.005 and ATP III (p = 0.001 criteria. CONCLUSION: The WHO criteria does not seem to be adequate for adolescents. IDF and ATP III criteria had an excellent agreement. Puberty and triglycerides were associated with MS.OBJETIVO: Avaliar a correlação de três critérios de síndrome metabólica (SM para adolescentes e identificar fatores associados. MÉTODOS: Estudo transversal com 65 pacientes obesos entre 10 e 18 anos do Ambulatório de Crianças e Adolescentes Obesos do HC-Unicamp. SM foi definida de acordo com a Organização Mundial da Saúde (OMS, International Diabetes Federation (IDF e Adult Treatment Panel III (ATP III. Buscaram-se fatores associados a SM em dados clínicos, antropométricos e laboratoriais. RESULTADOS: Dos 65 pacientes, nenhum foi diagnosticado como SM pela OMS, sendo 18 (27.6% pelo IDF e 19 (29.2% pelo ATP III. A correlação entre IDF e ATP III foi excelente (kappa 81%. Neste estudo, a