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Sample records for ressonancia magnetica hepatica

  1. Magnetic resonance imaging of the liver in postpartum stable women with HELLP syndrome; Ressonancia magnetica hepatica em puerperas estaveis com sindrome HELLP

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    Carvalho, Ana Rita Marinho Ribeiro; Amorim, Melania Maria Ramos de; Katz, Leila; Souza, Alex Sandro Rolland de; Santos, Aleksana Regina Viana Dutra; Lima, Ana Luiza Medeiros Vasconcelos de [Instituto Materno Infantil Prof. Fernando Figueira, Recife, PE (Brazil). Centro de Atencao a Mulher. Unidade de Terapia Intensiva Obstetrica

    2008-09-15

    Objectives: To describe magnetic resonance (MR) findings in the liver of stable patients with HELLP syndrome in the puerperium. Methods: A descriptive study was carried out from August 2005 to July 2006, involving a series of 40 postpartum patients admitted to an obstetric intensive therapy unit in IMIP (Instituto Materno Infantil Prof. Fernando Figueira) with diagnosis of HELLP syndrome (complete and partial). Complete HELLP syndrome was defined when all laboratory parameters were present and incomplete when one or more but not all laboratory findings were present. All patients had stable clinical conditions and were evaluated with magnetic resonance of the liver and the main findings were recorded. Results. Average maternal age was 26.8 {+-} 6.4 years and gestational age at delivery was 34 {+-} 26.8 weeks. The MR imaging was performed between eight and 96 hours after diagnosis of HELLP syndrome (56 {+-} 31h). The most frequent findings were ascitis in 20% (n = 8), pleural effusion in 17.5% and hepatic steatosis in 7.5%. The periportal intensity signal was normal in all cases. Cases of liver infarction and sub-capsular or parenchymatous hematoma were not observed. Conclusion: Findings of magnetic resonance imaging of the liver in stable HELLP syndrome postpartum patients were few and unspecific. Severe liver injuries such as parenchymatous or sub-capsular hematoma, entailing life risk were not found. Results do not corroborate the use of magnetic resonance as routine examination for stable patients with HELLP syndrome. (author)

  2. Magnetic resonance imaging evaluation of traumatic muscle injuries; Avaliacao por ressonancia magnetica das injurias musculares traumaticas

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    Dias, Elisa Pompeu [Santa Casa de Misericordia do Rio de Janeiro, RJ (Brazil); Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Radiologia; Coutinho Junior, Antonio Carlos; Domingues, Romulo Cortes; Domingues, Romeu Cortes [Centro Medico Barrashopping, Rio de Janeiro, RJ (Brazil). Clinica de Diagnostico por Imagem (CDPI)

    2001-12-01

    We evaluated 43 magnetic resonance scans of the leg or thigh of patients suffering from sports trauma. Strains were the most frequent lesions observed. These lesions presented iso- or hypointense signal on T1 and hyperintense signal on T2 images, and were classified according to the intensity of the injury of the fibers into grades 1, 2 and 3. The second most common lesions in these series were contusions that appeared iso- or hypointense on T1 and hyperintense on T2 images. Fibrosis was also observed as low signal lesions on T1 and T2 images. (author)

  3. Magnetic resonance imaging in schizophrenia: a morphometric study; Ressonancia magnetica na esquizofrenia: um estudo morfometrico

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    Castro, Claudio Campi de [Sao Paulo Univ., SP (Brazil). Instituto do Coracao (InCor). Secao de Ressonancia Magnetica]. E-mail: campi@uol.com.br

    2001-06-01

    Thirty-three patients with chronic schizophrenia and 21 normal subjects were submitted to magnetic resonance imaging studies using a 1.5 T scanner. Axial and coronal T 2-weighted images were obtained. The volumes of the brain, intracranial, supratentorial, infratentorial and the total, ventricular and subarachnoid cerebrospinal fluid volumes were measured using semi-automated morphometric methods. The volumes of the amygdala-hippocampus complex, para hippocampal gyrus cortex, putamen, globus pallidus, temporal lobe, gray and white matter of temporal lobe were also measured. These volumes were normalized using the intracranial volume as reference. The most relevant findings observed were reduced brain volume and increased total, ventricular and subarachnoid cerebrospinal fluid volumes in patients with schizophrenia when compared to the controls. Patients with schizophrenia had also smaller amygdala-hippocampus complexes, temporal lobes and temporal lobe white matter than the controls, as well as increased putamen volumes. (author)

  4. Meniscal configuration using magnetic resonance imaging; Configuracao meniscal pela ressonancia magnetica

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    Fernandes, Arthur da Rocha C.; Turrini, Elisabete; Karoauk, Teresa C.C.; Lederman, Henrique M. [Escola Paulista de Medicina, Sao Paulo, SP (Brazil). Dept. de Diagnostico por Imagem

    1997-04-01

    The authors present a review of the normal meniscal configuration and correlation with anatomic specimens. The images were obtained by magnetic resonance imaging. The images were obtained by magnetic resonance imaging. The authors emphasize the importance of knowing the relationship between the meniscus and the adjacent anatomic structures. (author) 31 refs., 10 figs., tabs.

  5. Characterization of functional polymers by NMR; Caracterizacao de polimeros funcionalizados por ressonancia magnetica nuclear

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    Neto, Oscar H.S. A.S.; San Gil, Rosane A.S.; Nakayama, T; Costa Neto, Claudio [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica

    1994-12-31

    Several synthetic polymers are used in the chemical analysis of complexes mixtures aiming to extract certain specific functional groups for further identification. This work describes the utilization of NMR in the characterization of one of the above mentioned compounds which will be used as reagent for the synthesis of another compound of the same type, which will be further used in the chemical analysis of alcohols and phenols. The methodology is described. The results are described and discussed 4 refs., 2 figs.

  6. Nuclear magnetic resonance of randomly diluted magnetic materials; Ressonancia nuclear magnetica em materiais magneticos diluidos aleatoriamente

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    Magon, C J

    1986-12-31

    The temperature dependence of the nuclear relaxation rates and line shapes of the F{sub O} resonance in the diluted antiferromagnet Fe{sub x} Zn{sub 1-x} F{sub 2} and Mn{sub x} Zn{sub 1-x} F{sub 2} are studied over a large temperature range T{sub N} < {approx} T {<=} 300 K. The high (room) temperature spin-lattice relaxation rates (1/T{sub 1}) of the F{sub O} nuclei, which are not transfer hyperfine coupled to the Fe (or Mn) spins, have been measured and calculated as a function of the concentration x. Good agreement with experiment is found for the theoretical results, which have been obtained in the range 0.1 {<=} x {<=} 0.8. The temperature dependence of 1/T{sub 1} for T{sub N}

  7. Characterization of natural bentonite by NMR; Caracterizacao de bentonitas naturais por ressonancia magnetica nuclear

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    Leite, Sidnei Q.M.; Dieguez, Lidia C [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia; Menezes, Sonia M.C. [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas; San Gil, Rosane A.S. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica

    1994-12-31

    Solid state NMR as well as several other instrumental chemical analysis techniques were used in order to characterize two natural occurring bentonite. The methodology is described. The NMR spectra, together with the other used techniques suggest that the observed differences are due to iron inclusions in tetrahedral and octahedral sites 5 refs., 3 figs., 5 tabs.

  8. Magnetic resonance imaging of cerebellar Schistosomiasis mansoni; Ressonancia magnetica na esquistossomose mansoni cerebelar

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    Braga, Bruno Perocco; Costa Junior, Leodante Batista da [Hospital da Baleia, Belo Horizonte, MG (BRazil). Servico de Neurocirurgia; Lambertucci, Jose Roberto [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Faculdade de Medicina. Servico de Doencas Infecciosas e Parasitarias

    2003-10-01

    A 15-year-old boy was admitted to hospital with a history of headache, dizziness, vomiting and double vision that started two weeks before. His parents denied any previous disease. During clinical examination he presented diplopia on lateral gaze to the left and horizontal nystagmus. No major neurological dysfunction was detected. He was well built, mentally responsive and perceptive. Laboratory findings revealed a leukocyte count of 10,000/mL, a normal red blood cell count and no eosinophilia. The magnetic resonance images (MRI) of the brain showed a left cerebellar lesion with mass effect compressing the surrounding tissues. Contrast-enhanced images showed a mass like structure and punctate nodules (Figures A and B: axial and coronal contrast-enhanced T1-weighted MR images showed the nodular - yellow arrows - enhancement pattern of a left cerebellar intraxial lesion). The lesion extended to the vermis and brachium pons and compressed the medulla. There was no hydrocephalus. He was taken to the operating room with the presumptive diagnosis of a neuroglial tumor, and submitted to a lateral suboccipital craniectomy. A brown, brittle tumoral mass without a clearly defined margin with the cerebellar tissue was removed. Microscopic examination revealed schistosomal granulomas in the productive phase in the cerebellum (Figure C). After surgery, treatment with praziquantel (50 mg/kg/dia, single dose) and prednisone (1 mg/kg/day) was offered and the patient improved quickly. Thirty days later he was seen again at the outpatient clinic: he was asymptomatic and with no neurological impairment. This is the eighth case of cerebellar involvement in schistosomiasis mansoni and the second report of a tumoral form of cerebellar schistosomiasis documented by magnetic resonance images. (author)

  9. Magnetic resonance imaging of pelvic endometriosis; Ressonancia magnetica em endometriose pelvica

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    Vieira, G.P.; Martin, B.; Tubiana, J.M. [Hospital Saint Antoine, Paris (France)

    1994-07-01

    Twenty-five magnetic resonance imaging (MRI) studies were performed in 18 patients with proven endometriosis. MRI findings were analyzed and compared with laparoscopic or surgical findings; MRI accurately demonstrated ovarian endometrial cysts as well as ectopic foci of endometriosis. Adhesions may be also suggested. Contrary to laparoscopy, MRI easily depicts both deep lesions and endometrial implants under the peritoneum. Consequently, MRI appears as an useful adjunct to laparoscopy for initial diagnosis before starting a medical treatment and above all as the imaging modality of choice for evaluation of the answer to treatment, avoiding iterative and often adhesions limited laparoscopies. (author). 7 refs.; 9 figs.

  10. Magnetic resonance imaging in sudden deafness; Ressonancia magnetica em surdez subita

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    Ramos, Hugo Valter Lisboa; Barros, Flavia Alencar; Penido, Norma de Oliveira; Souza, Ana Claudia Valerio de; Yamaoka, Wellington Yugo [Sao Paulo Univ., SP (Brazil). Dept. de Otorinolaringologia e Cirurgia da Cabeca e Pescoco; Yamashita, Helio [Sao Paulo Univ., SP (Brazil). Dept. de Imagem e Diagnostico]. E-mail: hvlramos@gmail.com

    2005-07-15

    The etiology of sudden deafness can remain undetermined despite extensive investigation. This study addresses the value of magnetic resonance imaging in the analysis of sudden deafness patients.Study Design: transversal cohort.Material And Method: In a prospective study, 49 patients attended at otolaryngology emergency room of Federal University of Sao Paulo - Escola Paulista de Medicina, from April 2001 to May 2003, were submitted to magnetic resonance imaging.Results: Magnetic Resonance abnormalities were seen in 23 (46.9%) patients and revealed two tumors suggestive of meningioma, three vestibular schwannomas, thirteen microangiopathic changes of the brain and five (21.7%) pathological conditions of the labyrinth.Conclusion: Sudden deafness should be approached as a symptom common to different diseases. The presence of cerebellopontine angle tumors in 10.2% of our cases, among other treatable causes, justifies the recommendation of gadolinium-enhanced magnetic resonance use, not only to study the auditory peripheral pathway, but to study the whole auditory pathway including the brain. (author)

  11. Seeking tools for image fusion between computed tomography, structural and functional magnetic resonance methods for applications in neurosurgery; Ferramentas para fusao de imagens dos metodos de tomografia computadorizada, ressonancia magnetica e ressonancia magnetica funcional para aplicacao pre-neurocirurgica

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    Rocha, Liana Guerra Sanches da, E-mail: liana@einstein.br [Departamento de Diagnostico por Imagem, Hospital Israelita Albert Einstein - HIAE, Sao Paulo (SP) (Brazil); Amaro Junior, Edson [Faculdade de Medicina, Universidade de Sao Paulo - USP, Sao Paulo, SP (Brazil). Deptartamento de Diagnostico por Imagem; Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil)

    2012-04-15

    To evaluate tools for the fusion of images generated by tomography and structural and functional magnetic resonance imaging. Methods: Magnetic resonance and functional magnetic resonance imaging were performed while a volunteer who had previously undergone cranial tomography performed motor and somatosensory tasks in a 3-Tesla scanner. Image data were analyzed with different programs, and the results were compared. Results: We constructed a flow chart of computational processes that allowed measurement of the spatial congruence between the methods. There was no single computational tool that contained the entire set of functions necessary to achieve the goal. Conclusion: The fusion of the images from the three methods proved to be feasible with the use of four free-access software programs (OsiriX, Register, MRIcro and FSL). Our results may serve as a basis for building software that will be useful as a virtual tool prior to neurosurgery. (author)

  12. Evaluation of epileptogenic focus in temporal lobe: correlation between ictal brain SPECT, magnetic resonance imaging and magnetic resonance spectroscopy; Avaliacao de foco epileptogenico do lobo temporal: correlacao entre SPECT ictal, ressonancia magnetica e ressonancia magnetica com espectroscopia de protons

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    Diegues, Maria Elena Martins [Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, RJ (Brazil). Servico de Medicina Nuclear]. E-mail: emartyns@terra.com.br; Pellini, Marcos Pinto; Alves-Leon, Soniza Vieira [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina; Domingues, Romeu Cortes [Clinica de Diagnostico por Imagem (CDPI), Rio de Janeiro, RJ (Brazil)

    2004-02-01

    The purpose of this study was to determine the degree of concordance between radiological and radioisotopic methods and, if positive, to evaluate the usefulness of ictal SPECT in the localization of the epileptogenic focus. Ictal brain SPECT, magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) were performed on six patients with refractory temporal lobe epilepsy. Ictal SPECT was performed after withdrawal of the anti-epileptogenic drugs during video-EEG monitoring, using {sup 99m}Tc-ECD, administered to patients at the time of the ictus. MRI was performed in T1, T2 and FLAIR sequences and MRS was obtained using the PRESS technique, with a single voxel positioned in both hippocampi. The statistical analysis included the determination of the values of Kappa (k), standard error (se) and significance level (p) for the lateralization of the ictal focus. The analysis of all findings was based on EEG localization of the ictal discharge, seizure duration (109-280 s; 152 s average) and time of radiotracer injection (30-262 s; 96 s average). We obtained correlated data in four patients (67 per cent) and values of k = 0.67, se = 0.38, and p 0.041. We concluded that there is a concordance between ictal SPECT, MRI and MRS data and the usefulness of the radioisotopic procedure is related to a non diagnostic EEG and when there is a discordant or misleading diagnosis after a comparative analysis of EEG and MRS. (author)

  13. Hallervorden Spatz syndrome: magnetic resonance findings. Case report; Sindrome de Hallervorden Spatz: achados na ressonancia magnetica. Relato de caso

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    Farage, Luciano; Castro, Mario Augusto Padula; Macedo, Tulio Augusto Alves [Universidade Federal de Uberlandia, MG (Brazil). Hospital de Clinicas. Setor de Radiologia; Assis, Marcelo Cardoso de [Universidade Federal de Uberlandia, MG (Brazil). Faculdade de Medicina. Dept. de Cirurgia; Souza, Lincoln Pereira de; Freitas, Luiz Oliveira de [Universidade Federal de Uberlandia, MG (Brazil). Dept. de Clinica Medica

    2004-09-01

    Hallervorden-Spatz syndrome is a neuro degenerative disease, autosomic recessive with two clinical features: early and late onset. Psychiatric, pyramidal and extrapyramidal signs are present in the late subtype. We report the case of a 41-old woman with extrapyramidal signs. Magnetic resonance imaging (MRI) showed the eye-of-the-tiger sign in the medial globus pallidus. This is due to a gliosis (increased signal) and accumulation of surrounding iron (decreased signal intensity) in long T R sequences. There is a strong relationship between MRI findings and the gene mutation responsible for this disease. It makes the MRI sensible for diagnosing this syndrome. (author)

  14. In vitro absolute metabolic quantification by magnetic resonance spectroscopy; Quantificacao metabolica absoluta in vitro por espectroscopia de ressonancia magnetica

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    Barreto, Felipe R.; Salmon, Carlos E.G. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Filosofia, Ciencias e Letras. Dept. de Fisica e Matematica], e-mail: garrido@ffclrp.usp.br

    2010-03-15

    In this project we estimated characteristic nuclear relaxation times (T1 and T2) from {sup 1}H in vitro of water and the following human brain metabolites: N-Acetyl-Aspartate (NAA), Choline (Cho), Creatine (Cr) and Myo-Inositol (M-Ins) using nuclear magnetic resonance spectroscopy, aiming at relaxation effects correction for absolute quantification. Phantoms with 300 mL of a standard solution with a metabolite were used for spectra acquisition. Variable eco time sequences were used to determinate T2 while variable repetition or inversion time was used for T1 determination. Spectra processing was done with MRUI and LCModel software. Origin 8.0 was used for data fitting. The following T1/T2 values (ms) were the best estimated according to calculated absolute concentration analyses: (4061/934) for water, (1800/830) for NAA, (2316/717) for Cr, (2356/1095) for Cho and (1537/531) for M-Ins. With MRUI better results were obtained in T1 and T2 determination for relaxation effects correction in the absolute quantification process. (author)

  15. Nuclear magnetic resonance of D(-)-{alpha}-amino-benzyl penicillin; Ressonancia magnetica nuclear da D(-)-{alpha}-amino-benzil penicilina

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    Aguiar, Monica R.M.P.; Gemal, Andre L.; San Gil, Rosane A.S. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica; Menezes, Sonia M.C. [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas

    1995-12-31

    The development of new drugs from penicillins has induced the study of this substances by nuclear magnetic resonance. Several samples of D(-)-{alpha}-amino-benzyl penicillin were analysed using {sup 13} C NMR techniques in aqueous solution and solid state. Spectral data of this compounds were shown and the results were presented and analysed 7 figs., 4 tabs.

  16. Use of magnetic resonance imaging in the diagnosis of chondromalacia patella; Ressonancia magnetica no diagnostico da condromalacia de patela

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    Freire, Maxime Figueiredo de Oliveira; Fernandes, Artur da Rocha Correa; Freire Filho, Edison de Oliveira; Szejnfeld, Jacob [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: maximefreire@ig.com.br; Juliano, Yara; Novo, Neil Ferreira [Universidade Santo Amaro (UNISA), SP (Brazil). Faculdade de Medicina; Carneiro Filho, Mario [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Ortopedia e Traumatologia; Silva, Debora da Costa [Universidade Santo Amaro (UNISA), SP (Brazil). Faculdade de Odontologia

    2006-01-15

    Patellar articular cartilage lesions are very common and due to the dissociation between signs and symptoms and the stage of the chondral lesions the diagnostic method of choice is magnetic resonance imaging. Therefore, radiologists should be familiar with chondral abnormalities in order to correctly diagnose this condition. (author)

  17. Application of nuclear magnetic resonance in osteoporosis evaluation; Aplicacoes de ressonancia magnetica nuclear na avaliacao de osteoporose

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    Giannoni, Ricardo A., E-mail: giannoni@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Montrazi, Elton T.; Bonagamba, Tito J., E-mail: elton.montrazi@gmail.com, E-mail: tito@ifsc.usp.br [Universidade de Sao Paulo (IFSC/USP), Sao Carlos, SP (Brazil). Inst. de Fisica; Cesar, Reinaldo, E-mail: reinaldofisica@gmail.com [Universidade de Sao Paulo (EESC/USP), Sao Carlos, SP (Brazil). Escola de Engenharia

    2013-07-01

    In this work, initially ceramic samples of known porosity were used. These ceramic samples were saturated with water. The nuclear magnetic resonance signal due to relaxation processes that the hydrogen nucleus water contained in the pores of this ceramic material was measured. Then these samples were subjected to a process of drying and measures successively. As the water contained in pores greater evaporates the intensity of signal decreases and shows the sign because of the smaller pores. The analysis of this drying process gives a qualitative assessment of the pore size of the material. In a second step, bones of animals of unknown porosity underwent the same methodology for evaluating osteoporosis. Also a sample of human vertebra in a unique manner, with the same purpose was measured. Combined with other techniques is a quantitative evaluation of the possible porosity.

  18. Design of radiofrequency coils for NMR imaging; Desenvolvimento de bobinas de radiofrequencia para geracao de imagens por ressonancia magnetica nuclear

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    Schmidt, Maria Angelica

    1988-07-01

    In this work we describe and analyse different types of radiofrequency coils for NMR Imaging. Our system operates with a superconducting magnet, 22.5 cm bore, at 2.0 Tesla (85 MHz, resonant frequency for protons). Distinct structures, frequently described in the specialized literature, are discussed here. Usually the RF coils proposed are resonant circuits with distributed inductance and capacitance. We have built different types of resonators and its performance were compared. With the resonators developed here we have been able to obtain proton density images of good quality. (author)

  19. Nuclear magnetic resonance in superionic conductors with fluorite-type structure; Ressonancia magnetica nuclear em condutores superionicos de estrutura fluorita

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    Souto, Sergio Paulo Amaral

    1991-12-31

    The {sup 19} F NMR relaxation times T{sub 1} and T{sub 2} were measured in ternary and nonstoichiometric compounds with the fluorite-type structure. We have studied the Na{sub 0-4} Y{sub 0-6} F{sub 2-2} crystal in the temperature range 600 K to 900 K, where the crystal has not the fluorite structure. The T{sub 1} values were measured in 2 Larmor frequencies: 20.42 MHz and 34.24 MHz. The results for T{sub 1} were seem to be qualitatively similar to those measured in the system with two inequivalent sublattices. The T{sub 2} measurements, in the Pb{sub O84} Bi{sub 0-16} F{sub 2-16} crystal, were made during temperature cycles in the range of 300 K to 830 K. The difference in activation energy between cooling and heating half cycles, found to be approximately 0.08 eV, appear to be associated with the change in the clusters structure and not to the energy of defect formation. Finally, similar T{sub 2} measurements during temperature cycling was made in K{sub 0-4} Bi{sub 0-6} F{sub 2-2} : 2% Pb F{sub 2} crystal, in the temperature range 300 K to 800 K, but in this case no difference in the cooling and heating results was observed. We also measured, in the same temperature range, the T{sub 1} relaxation time in 3 Larmor frequencies: 11.71 MHz, 20.42 MHz and 34.24 Mhz. This results appear to indicate the existence of two hopping mechanism. (author). 132 refs., 68 figs.

  20. High resolution spectroscopy in solids by nuclear magnetic resonance; Espectroscopia de alta resolucao em solidos por ressonancia magnetica nuclear

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    Bonagamba, T J

    1991-07-01

    The nuclear magnetic resonance (NMR) techniques for High Resolution Spectroscopy in Solids are described. Also the construction project of a partially home made spectrometer and its applications in the characterization of solid samples are shown in detail. The high resolution spectrometer used is implemented with the double resonance multiple pulses sequences and magic angle spinning (MAS) and can be used with solid and liquid samples. The maximum spinning frequency for the MAS experiment is in excess of 5 Khz, the double resonance sequences can be performed with any type of nucleus, in the variable temperature operating range with nitrogen gas: -120{sup 0} C to +160{sup 0} C, and is fully controlled by a Macintosh IIci microcomputer. (author).

  1. Characterization of a tannin by NMR; Caracterizacao de um tanino elagico por espectrometria de ressonancia magnetica nuclear

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    Teixeira, Antonio C.F.; Silva, Antonio J.R. da [Universidade Federal, Rio de Janeiro (Brazil). Nucleo de Pesquisas de Produtos Naturais

    1994-12-31

    This work presents the observations performed during the process of characterization of Geraniin, a crystalline tannin, which is able to undergo hydrolysis, isolated from Phyllanthus tennellus, a plant known in Brazil as `quebra-pedra`. The methodology is presented and the chemical analysis performed for the identification are presented and discussed. The obtained results are compared to the existing literature 5 refs., 2 figs., 8 tabs.

  2. Proceedings of the nuclear magnetic resonance user meeting. Anais do Encontro de Usuarios de Ressonancia Magnetica Nuclear

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    1987-01-01

    Studies on utilization of nuclear magnetic resonance, such as: chemical analysis in complexes and organic compounds; structures and magnetic properties of solids; construction of images and; spectrometer designs, are presented. (M.C.K.).

  3. Nuclear magnetic resonance studies in solid polymeric electrolyte. Estudos por ressonancia magnetica nuclear em eletrolitos polimericos solidos

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    Donoso, J P; Mattoso, L H.C.; Cavalcante, M G [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Fisica e Quimica; Gorecki, W; Berthier, C; Armand, M [Universite J. Fourier, Grenoble (France)

    1990-01-01

    Solid complexes formed between Poly (ethylene oxide) and various alkali metal salts, are generally referred to as polymer electrolytes conductivity and NMR properties were investigated in POE - Li Cl sub(7)O sub(4) and POE - Li BF sub(4) complexes. Our sup(1)H, Li and sup(19)F relaxation study suggest that cation motion is controlled by segmental motions of the polymer chain while the anion have additional mobility associated with BG sub(4) rotation. (author).

  4. Selection of multislice in nuclear magnetic resonance tomography. Selecao de multiplos planos em tomografia por ressonancia magnetica nuclear

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    Frare, Junior, P L

    1990-01-01

    The study and the implementation of three techniques for the selection of multislice, aiming the optimization of the NMR tomography is presented. The first technique uses the simultaneous excitation of multiple slices, being each plane encoded with a pre determined phase for a latter decodification of the information by the linear combination of n experiments. The second one makes use of the multislice simultaneous excitation, like the first one, but the slices are acquired in the presence of an oblique reading gradient, which provides us the multi slices signal without any further computer processing. The third technique uses frequency multiplexed excitation, that is, different slices are successively excited during the repetition time of an acquisition, making possible multislice acquisition at the same time of a single slice. The construction and the use of a phantom for the equipment characterization are discussed too. Many technical aspects necessary for an improvement of the tomography performance, like reception circuits and resonators, are discussed. A brief introduction to the gradient modulated adiabatic excitation (GMAX) technique and the first results ever obtained with it, using surface coils, is presented. (author).

  5. Intracranial meningiomas: magnetic resonance imaging findings in 78 cases; Meningiomas intracranianos: achados de ressonancia magnetica em 78 casos

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    Gasparetto, Emerson L.; Leite, Claudia da Costa; Lucato, Leandro T.; Barros, Cristiano Ventorin de [University of Sao Paulo (USP), SP (Brazil). School of Medicine. Dept. of Radiology]. E-mail: egasparetto@gmail.com; Marie, Sueli K.N.; Santana, Pedro; Aguiar, Paulo Henrique Pires de [University of Sao Paulo (USP), SP (Brazil). School of Medicine. Dept. of Neurology; Rosemberg, Sergio [University of Sao Paulo (USP), SP (Brazil). School of Medicine. Dept. of Pathology

    2007-09-15

    Objective: To present the magnetic resonance (MR) imaging findings of 78 patients with meningiomas diagnosed in a single institution. Method: 78 patients with histological proven intracranial meningioma were studied. There were 52 female and 26 male patients (median=56 years). All MR imaging examinations were performed with 1.5-T MR imaging unit with standard protocol. The images were studied by two neuroradiologists, who reached the decisions regarding the findings by consensus. Results: Most of the tumors showed low signal on T1- (60%) and high signal on T2- (68%) and FLAIR (69%) weighted images. Also, the lesions showed heterogeneous signal on T1 (60%), T2 (68%) and FLAIR (64%) sequences. After contrast administration, 83% (n=65) of the tumors presented accentuated and 17% (n=13) showed moderate enhancement. The tumors were located in the frontal lobe in 44% of the cases, in the parietal lobe in 35%, the occipital lobe in 19% and the temporal lobe in 12% of the patients. Areas of vasogenic edema around the tumors were seen in 90% of the cases. Twenty six per cent of the cases showed bone infiltration, and the dural tail sign was seen in 59% of the tumors. Conclusion: Intracranial meningiomas usually show heterogeneous low signal on T1- and high signal on T2-weighted and FLAIR images, with intense enhancement after contrast administration. The frontal and parietal lobes are commonly affected. In addition, brain edema, dural tail sign and bone infiltration are the most frequent associated findings. (author)

  6. Magnetic resonance imaging in lower urinary tract endometriosis: iconographic essay; Ressonancia magnetica na endometriose do trato urinario baixo: ensaio iconografico

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    Lima, Claudio Marcio Amaral de Oliveira; Coutinho, Elisa Pompeu Dias; Ribeiro, Erica Barreiros; Domingues, Marisa Nassar Aidar; Junqueira, Flavia Pegado; Coutinho Junior, Antonio Carlos [Clinicas de Diagnostico Por Imagem (CDPI) e Multi-Imagem, Rio de Janeiro, RJ (Brazil)], e-mail: cmaol@br.inter.net, e-mail: cmaolima@gmail.com

    2009-05-15

    Endometriosis is defined as the presence of functional endometrial tissue outside the endometrial cavity and myometrium. Although this is a frequent disease with multifactorial causes, involvement of the lower urinary tract is rare. Magnetic resonance imaging is highly sensitive, specific and accurate in the diagnosis of endometriosis in the lower urinary tract, especially for allowing the identification of lesions obscured by adhesions or with subperitoneal extension. The present iconographic essay presents the main magnetic resonance imaging findings of the lower urinary tract involvement by endometriosis. (author)

  7. Magnetic resonance imaging of racemous cysticercosis of the cauda equina; Ressonancia magnetica de paciente com cisticercose racemosa da cauda equina

    Energy Technology Data Exchange (ETDEWEB)

    Costa Junior, Leodante Batista da; Lemos, Sandro Pedroso [Hospital da Baleia, Belo Horizonte, MG (Brazil). Servico de Neurocirurgia; Lambertucci, Jose Roberto [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Faculdade de Medicina. Servico de Doencas Infecciosas e Parasitarias

    2003-12-01

    37-year-old man presented with low back pain for the last 6 months followed by sensation of numbness in the lower limbs that evolved with paraparesis, urinary retention and impotence. Subsequently, he lost control over the bowel function. Neurological examination revealed increased muscle tone along with grade zero power in both lower limbs. Knee and ankle jerks were exaggerated. Nine months prior to admission, a type II diabetes mellitus was diagnosed and he has been taking insulin ever since. MRI revealed multiple cystic lesions in the cauda equina opposite the L1-S1 vertebral bodies with no involvement of the spinal cord (Figure A: sagittal section T1-weighted image after contrast with cystic lesions on the left side - yellow arrow -, and T2-weighted image on the right - red arrows pointing cystic lesions). The cysts were hypointense on T1-weighted images (Figure B: horizontal section - yellow arrows) and hyperintense in T2-weighted images (Figure A - red arrows). Computerized tomography of the brain showed moderate ventricle dilatation (Figure C) with no intra or extra axial lesions. He was submitted to laminectomy and the cysts were surgically removed. An intense inflammatory process (arachnoiditis) involving the nerve roots of the cauda equina was reported. Histology of the surgical specimen confirmed the diagnosis of racemous cysticercosis. He improved quickly after surgery. Three months later, at the outpatient clinic, he walked with support, resumed sphincter control of the bladder and bowel and had no more pain. Ten months later he returned to hospital walking with crutches, with hypoesthesia and paraesthesia on the left leg and urinary incontinence. He refused treatment with albendazole and steroids. (author)

  8. DETEKSI Fasciola hepatica DAN Fasciola gigantica PADA KERBAU PERAH ( DETEKSI Fasciola hepatica DAN Fasciola gigantica PADA KERBAU PERAH ( DETEKSI Fasciola hepatica DAN Fasciola gigantica PADA KERBAU PERAH (

    OpenAIRE

    -, YAUMIL NI???MAH YAUMIL NI???MAH YAUMIL NI???MAH

    2016-01-01

    2015 ABSTRAK YAUMIL NI???MAH O11111101.Deteksi Fasciola hepatica dan Fasciola gigantica pada Kerbau Perah Fasciola hepatica dan Fasciola gigantica merupakan cacing kelas trematoda.Telur cacing ini berbentuk oval dan dilengkapi dengan operculum yang berfungsi sebagai jalan keluar larva mirasidium pada saat telur menetas.Ukuran telur Fasciola gigantica lebih besar dibandingkan ukuran telur Fasciola hepatica.Penelitian ini bertujuan untuk mendeteksi telur cacing Fasciola...

  9. Imagenoligía para el siglo XXI resonancia magnetica

    OpenAIRE

    Mejía González, Mauricio; Fundación Valle de Lili

    1998-01-01

    ¿Qué es la resonancia magnetica?/¿Cómo funciona la resonancia magnética?/¿Para que se utiliza la resonancia magnética?/¿En que casos no se debe hacer resonancia magnética?/¿Se puede hacer resonancia a niños o mujeres embarazadas?/¿Qué se siente durante el examen?/¿Se requiere de medio de contraste?/¿Qué preparación previa se debe tener?/¿Que ventajas tiene la resonancia sobre otros métodos para imágenes diagnósticas?

  10. Hepatic Mass Caused by Fasciola Hepatica: A Tricky Differential Diagnosis

    Science.gov (United States)

    Yılmaz, Bülent; Köklü, Seyfettin; Gedikoğlu, Gökhan

    2013-01-01

    Fascioliasis is a zoonotic infestation caused by the trematode fasciola hepatica. It presents a wide spectrum of clinical pictures ranging from fever and eosinophilia to ambiguous gastrointestinal symptoms in the acute phase. However, it may often be overlooked, especially in the acute phase, because of uncertain symptoms. Fasciola hepatica can have an initial presentation similar to the presentation of malignancy. Here, we report a case of a hepatic mass caused by fasciola hepatica. PMID:24080633

  11. State of the knowledge of flora of hepaticas of Colombia

    International Nuclear Information System (INIS)

    Uribe M, Jaime; Gradstein, S Rob

    1999-01-01

    The Colombian hepatic flora is a very rich one. About 60% of all the species of tropical America and one sixth of the world's hepaticas occur in Colombia. The country has more species than the whole of Africa south of the Sahara and almost twice as many as Europe. The new catalogue of the hepaticas of Colombia accepts 840 species (in 36 families and 136 genera): 832 species of Hepaticae and 8 species of Anthocerotae

  12. Seroprevalence of Fasciola hepatica in cattle in Estonia

    DEFF Research Database (Denmark)

    Petersson, Jennifer; Jokelainen, Pikka; Lassen, Brian

    2017-01-01

    Fasciolosis, an infectious disease caused by the liver fluke Fasciola hepatica, affects grazing cattle world-wide. Liver fluke F. hepatica is prevalent and well-documented in cattle in many European countries, but for the Baltic countries such information is limited. This study investigated...

  13. Magnetic resonance imaging findings in patients with Hallervorden-Spatz syndrome; Achados de ressonancia magnetica na sindrome de Hallervorden-Spatz

    Energy Technology Data Exchange (ETDEWEB)

    Nomura, Cesar Higa; Lima, Eduardo Carneiro; Cerri, Giovanni Guido; Leite, Claudia da Costa [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Radiologia; Rosemberg, Sergio [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Patologia

    2003-12-01

    The objective of this study was to review the magnetic resonance imaging findings in patients with Hallervorden-Spatz syndrome. We evaluated eight patients with Hallervorden-Spatz syndrome using magnetic resonance imaging. The protocol included at least sagittal and axial T1-weighted images and axial and coronal T2-weighted images. Intravenous gadolinium was administered in a dose of 0.1 mmol/kg. Post-enhanced images were obtained at least in the axial and coronal planes. The results were: all patients presented a bilateral and symmetrical 'tiger-eye signal' on T2-weighted images, corresponding to central hyperintensity and peripheral hypointensity in the globi pallidus. FLAIR and diffusion images showed similar abnormalities. There was no gadolinium enhancement in any of the cases. We concluded that magnetic resonance imaging findings in patients with Hallervorden-Spatz syndrome are very typical and allow the diagnosis of the disease. (author)

  14. Leukokoria in childhood: differential diagnosis using ultrasonography, computed tomography, and magnetic resonance imaging; Leucocoria na infancia: diagnostico diferencial por ultra-sonografia, tomografia computadorizada e ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Montandon Junior, Marcelo Eustaquio; Montandon, Cristiano [Colegio Brasileiro de Radiologia e Diagnostico por Imagem (CBR), Sao Paulo, SP (Brazil)]. E-mail: marmontandon@hotmail.com; Figueiredo, Sizenildo da Silva; Ribeiro, Flavia Aparecida de Souza; Nobrega, Bruno Barcelos da; Lobo, Leonardo Valadares Barbosa; Teixeira, Kim-Ir-Sen Santos [Goias Univ., Goiania (Brazil). Hospital das Clinicas. Servico de Diagnostico por Imagem e Anatomia Patologica; Jacob, Beatriz Mahmud [Goias Univ., Goiania (Brazil). Faculdade de Medicina. Radiologia e Diagnostico por Imagem

    2004-04-01

    Leukokoria is an abnormal pupillary reflex most commonly seen in children that usually results from an intra-ocular abnormality. The evaluation of a child with leukokoria should be performed particularly to rule out a retinoblastoma (the cause of leukokoria in half of the cases). The authors present the main clinical and imaging features for the diagnosis of the most common causes of leukokoria. (author)

  15. Magnetic resonance imaging of the hip in rheumatoid arthritis patients: a descriptive study; A ressonancia magnetica do quadril na avaliacao de pacientes com artrite reumatoide: estudo descritivo

    Energy Technology Data Exchange (ETDEWEB)

    Caldana, Wanda Chiyoko Iwakami; Fernandes, Artur da Rocha Correa [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: wiwakami@ig.com.br; Ciconelli, Rozana Mesquita [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil); Juliano, Yara [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Epidemiologia e Bioestatistica

    2005-07-15

    Objective: To assess the usefulness of MRI as a diagnostic method in patients with rheumatoid arthritis, and to evaluate the frequency of changes in the hip in symptomatic and asymptomatic patients. Materials and methods: Forty patients (17 symptomatic and 23 asymptomatic) were evaluated by 1.5 T magnetic resonance imaging (MRI) after intravenous contrast media injection. MRI findings were divided in inflammatory, degenerative, combined (inflammatory and degenerative) and extra-articular changes. Results: The most frequent findings seen were degenerative and extra-articular changes (22.5% each). Inflammatory changes were seen in only five (12.5%) symptomatic patients, four of them with degenerative findings associated (10%). Conclusion: The use of MRI of the hip allowed the evaluation of the main articular abnormalities seen in patients with rheumatoid arthritis. The most frequent findings were degenerative and extra-articular changes. Inflammatory findings were seen in only five patients, four of them with degenerative changes associated. (author)

  16. Magnetic resonance imaging pre- and postoperative evaluation of tetralogy of Fallot; Avaliacao pre e pos-operatoria da tetralogia de Fallot por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Bernardes, Renata Junqueira Moll; Simoes, Luiz Carlos [Instituto Nacional de Cardiologia (INC), Rio de Janeiro, RJ (Brazil). Servico de Cardiologia da Crianca e do Adolescente; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia]. E-mail: edmarchiori@bol.com.br; Bernardes, Paulo Manuel de Barros; Gonzaga, Maria Beatriz Albano Monzo [Rede Labs/D' Or, Rio de Janeiro, RJ (Brazil)

    2004-08-01

    The purpose of this study was to assess the usefulness of magnetic resonance imaging (MRI) in the pre- and postoperative evaluation of patients with tetralogy of Fallot. Twenty patients aged 1 to 29 years were prospectively evaluated with black-blood and contrast-enhanced angiographic techniques, 11 with the classic form of tetralogy of Fallot and 9 with tetralogy of Fallot and pulmonary atresia. MRI studies provided adequate visualization of the aorta that was classified as dilated or not dilated, and definition of its position in all cases. The use of contrast-enhanced MR angiographic techniques provided excellent imaging of the main right and left pulmonary arteries. The results suggest that MRI, including contrast-enhanced angiography techniques, is a useful tool in the evaluation of patients with tetralogy of Fallot before and after cardiac surgery since it provides important anatomical information that is not always obtained with echocardiography. MRI can be considered an alternative to cardiac catheterization, particularly in the evaluation of the pulmonary vascular anatomy. (author)

  17. Proceedings of the 10. Brazilian meeting on magnetic resonance. Abstracts and program; Anais da 10. Jornada brasileira de ressonancia magnetica. Resumos e programa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    This annual meeting, held in Brazil from August 4 - 8, 2008 comprised: one mini-course about the use of nuclear magnetic resonance for structural chemical analyses); four major conferences ('NMR applied to molecular dynamics studies of solids and semi-solids', 'Beyond genomes: assessing protein function using solution NMR', 'NMR applied to natural products', 'Solid-state NMR as a tool for the investigation of membrane-associated peptides and proteins'), four short conferences ('NMR applied to petroleum, derivatives and biofuels', 'NMR of solids applied to pharmaceutical drugs', 'NMR applied to agricultural and cattle raising quality control', 'NMR applied to oil wells logging'); oral presentations of three undergrad, three M.Sc., and three Ph.D. projects; nine other research works presented as oral communications and fifty two as congress panels/posters), most being carried out by scientific groups from various Brazilian R and D institutions. Prizes to nine remarkable works of undergrad and post-grad students have been awarded, and a General Assembly meeting of AUREMN, the Brazilian Association of Nuclear Magnetic Resonance Users, also took place at the end of the event. Main topics of the research works presented at this meeting, both as oral communications and as congress panels, were thus distributed: 24% in chemical sciences (mainly organic chemistry), 45% in applied life sciences (agricultural and food sciences, biological sciences and medicine), 29% in materials science (including petroleum and alternative fuels), and 2% regarding development of equipment accessories and improvement of instrumental techniques.

  18. Temporomandibular joint - normal features and disc displacements: magnetic resonance imaging; Articulacao temporomandibular - aspectos normais e deslocamentos de disco: imagem por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Ramos, Ana Carolina Araujo; Sarmento, Viviane Almeida; Campos, Paulo Sergio Flores; Gonzalez, Maria Olivia Dias [Bahia Univ., Salvador, BA (Brazil). Faculdade de Odontologia]. E-mail: ana_carolinaramos@hotmail.com

    2004-12-01

    Disc displacement of the temporomandibular joint has been defined as an abnormal relationship between the articular disc and the mandibular condyle, fossa and articular eminence. Disc displacements may occur without immediate interference in the function of the joint. Magnetic resonance imaging shows high diagnostic accuracy in the determination of articular disc position and has been indicated as the diagnostic method of choice for soft tissue abnormalities of the temporomandibular joint. The aim of this study is to review the literature including the normal features and different types of disc displacement of the temporomandibular joint as well as the imaging findings, emphasizing the role of magnetic resonance imaging. (author)

  19. Magnetic resonance imaging of sella turcica: evaluation of patients with galactorrhea, amenorrhea and hyperprolactinemia; Ressonancia magnetica da sela turca: avaliacao de pacientes com galactorreia, amenorreia e hiperprolactinemia

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Alair Augusto Sarmet M.D. dos [Hospital Universitario Antonio Pedro, Niteroi, RJ (Brazil). Dept. de Radiologia; Moreira, Denise Madeira [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia; Andreiuolo, Pedro Angelo [Beneficencia Portuguesa de Niteroi, RJ (Brazil). Servico de Radiologia Santa Cruz Scan

    1999-08-01

    We have selected 135 cases of patients who have done magnetic resonance imaging of sella region, carried out from September, 1991 to August, 1996, who had galactorrhea (G), amenorrhea(A), and hyperprolactinemia (H), isolated or in association. The patients were divided in seven groups, according to the presence of these symptoms and signs. All examinations were made in a private clinic in the city of Rio de Janeiro, Brazil. Correlating these patients with the results of the magnetic resonance images, we found 57 micro adenomas, 31 normal examinations, 22 macro adenomas, 11 pituitary hyperplasias, 7 empty sella and 7 cases included in other aspects. The micro adenoma predominated in groups 1 (GAH), 2 (GH), 3 (HA) and 5 (H), that is, in all groups whose patients had hyperprolactinemia. In macro adenomas, a bright signal on T 1-weighted images indicates pituitary apoplexy with intratumoral hemorrhage. All in all, the magnetic resonance imaging is excellent method to be used in the evaluation of patients with changes in the hypothalamic-pituitary axis. (author)

  20. Corpus callosum dysgenesis and lipoma: embryologic and magnetic resonance imaging aspects; Disgenesia do corpo caloso e lipoma: consideracoes embriologicas basicas e aspectos de imagem a ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Abreu Junior, Luiz de; Borri, Maria Lucia; Wolosker, Angela Maria Borri; Hartmann, Luiz Guilherme de Carvalho; Galvao Filho, Mario de Melo [Hospital e Maternidade Sao Luiz, Sao Paulo, SP (Brazil). Setor de Diagnostico por Imagem]. E-mail: abreujr@directnet.com.br; D' Ippolito, Giuseppe [Hospital e Maternidade Sao Luiz, Sao Paulo, SP (Brazil). Setor de US/TC/RMN

    2005-07-15

    The corpus callosum is the major system of association fibers that permits communication of both cerebral hemispheres. Magnetic resonance imaging has improved the study of brain malformations, including the corpus callosum dysgenesis. Lipoma is a common finding in the spectrum of corpus callosum dysgenesis. The purpose of these study was to review the embryologic events and the magnetic resonance imaging aspects related to the corpus callosum dysgenesis and to the formation of the related lipoma. (author)

  1. Bone injuries in the post-traumatic knee: evaluation by magnetic resonance imaging; Comprometimento osseo do joelho pos-trauma: avaliacao pela ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    De Grossi, Carla Martins [Universidade Federal, Rio de Janeiro, RJ (Brazil). Curso de Pos-graduacao em Radiologia; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Servico de Radiologia; Santos, Alair Augusto S.M.D. dos [Hospital Santa Cruz, Niteroi, RJ (Brazil). Servico de Radiologia]. E-mail: edmarchiori@zipmail.com.br

    2001-06-01

    This paper presents the results of 50 patients with trauma of the knee submitted to magnetic resonance imaging in the period of January 1996 to December 1997. The appearance and incidence of the main bone lesions were evaluated and correlated with the mechanisms of aggression and with clinical data. Associated lesions were also evaluated. Bone contusions were the most commonly findings encountered and were observed in 38 patients (76%). Osteochondral fractures occurred in five patients (10%) and bone fractures were detected in five patients (10%), of which two were associated with contusions in other adjoining bone compartments. Chondromalacia of the patella was observed only in two patients (4%). We concluded that magnetic resonance imaging is the imaging method of choice for the diagnosis of bone lesions in patients with trauma of the knee. (author)

  2. Magnetic resonance imaging textural evaluation of posterior cranial fossa tumors in childhood; Avaliacao textural por ressonancia magnetica dos tumores da fossa posterior em criancas

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Joelson Alves dos; Costa, Maria Olivia Rodrigues da; Otaduy, Maria Concepcion Garcia; Lacerda, Maria Teresa Carvalho de; Leite, Claudia da Costa [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Radiologia]. E-mail: joelson_alves@ig.com.br; Matsushita, Hamilton [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Neurologia

    2004-08-01

    Objective: To distinguish healthy from pathological tissues in pediatric patients with posterior cranial fossa tumors using calculated textural parameters from magnetic resonance images. Materials And Methods: We evaluated 14 pediatric patients with posterior cranial fossa tumors using the software MaZda to define the texture parameters in selected regions of interest representing healthy and pathological tissues based on T2-weighted magnetic resonance images. Results: There was a statistically significant difference between normal and tumoral tissues as well as between supposedly normal tissues adjacent and distant from the tumoral lesion. Conclusion: Magnetic resonance textural evaluation is an useful tool for determining differences among various tissues, including tissues that appear apparently normal on visual analysis. (author)

  3. Evaluation of poly(vinylpyrrolidone) and collagen by Low Field Nuclear Magnetic Resonance Spectroscopy; Avaliacao da polivinilpirrolidona e do colageno por ressonancia magnetica nuclear de baixo campo

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Paula de M.; Tavares, Maria I.B. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Macromoleculas Professora Eloisa Mano]. E-mail: pmcosta@ima.ufrj.br

    2005-07-01

    Blends of natural and synthetic polymers represent a new class of materials with better mechanical properties and biocompatibility than those of the single components. Collagen and poly(vinylpyrrolidone) are well known for their important biological properties. The blending of collagen with poly(vinylpyrrolidone) makes it possible to obtain new materials in which strong interactions between the synthetic and biological components occur. Do to the excellent biocompatibility of these polymers, this blend has been much studied intending biomedical applications. And a one technique that can provide important information on molecular mobility, compatibility and even evaluate the interactions that can occur with these polymers is the Low Field Nuclear Magnetic Resonance Spectroscopy. Thus, the purpose of this work is to evaluate collagen and poly(vinylpyrrolidone) by Low Field Nuclear Magnetic Resonance Spectroscopy. From the values of relaxation times obtained, we can conclude that these materials have different interactions, and different mobility domains, confirming the heterogeneity and complexity of these materials. (author)

  4. Dosimetry for computed tomography using Fricke gel dosimetry and magnetic resonance imaging; Dosimetria em tomografia computadorizada empregando dosimetro Fricke gel e a tecnica de imageamento por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Capeleti, Felipe Favaro; Campos, Leticia L., E-mail: felipe@gmpbrasil.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2014-04-15

    In this work it was determined a new method for the determination of absorbed doses in Computed Tomography (CT) examinations using Fricke gel dosimetry developed at IPEN. Absorbed doses were determined by different methods of analysis, such as optical absorption spectrometry, ionization chambers and magnetic resonance imaging. Lower limit of sensitivity of the Fricke gel solution, the solution repeatability signal Fricke gel and CT equipment, detection sensitivity, among other tests were performed. Different equipment of computed tomography with multiple detectors were used. The Fricke gel solution showed better repeatability than ±5.5% using the technique of optical absorption spectrophotometry and computed tomography equipment showed repeatability better than ±0.2%. The Fricke gel solution features an easy and relatively quick preparation, but it is necessary to be careful not to contaminate and lose the solution. With the results, it was confirmed the application of this type of dosimetry for computed tomography equipment. (author)

  5. 12. Brazilian meeting on magnetic resonance; mini-course in NMR. Abstracts and program; 12. Jornada brasileira de ressonancia magnetica; mini-curso em RMN

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-07-01

    Theoretical and experimental papers are presented in these proceedings comprehending the following subjects: nuclear magnetic resonance, organic and non organic compounds, polymers, petroleum, physical chemistry, chemical structures, molecular biology, molecular structures and proteins.

  6. Use of nuclear magnetic resonance in the characterization of modified clays; Uso da ressonancia magnetica nuclear na caracterizacao de argilas modificadas

    Energy Technology Data Exchange (ETDEWEB)

    Leite, Sidnei Q.M. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia; Menezes, Sonia M.C. de [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas; Gil, Rosane A.S. San [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica

    1992-12-31

    This work uses NMR spectroscopy with rotation in the {sup 29} Si, {sup 27} Al and {sup 23} Na magic angles for characterization of clays with Si/Al ratio in the range 3.0 - 16,5, obtained by chemical treatment of commercial samples, under different conditions of leaching with mineral acid 5 refs., 2 figs., 2 tabs.

  7. Proceedings of the 4. Meeting of the nuclear magnetic resonance users; Anais do 4. Encontro de usuarios de ressonancia magnetica nuclear

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-31

    This book shows the papers presented in the 4. Meeting of Nuclear Magnetic Resonance Users which took place in Rio de Janeiro, Brazil, 1993. The main topics discussed were: solid state NMR; structural and conformational structure determination by NMR; perspectives for NMR in Brazil; recent achievements in NMR

  8. Determination of the composition of a mixture of polar substances by NMR; Determinacao da composicao de uma mistura de substancias polares por espectrometria de ressonancia magnetica nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Antonio C.F.; Silva, Antonio J.R. da [Universidade Federal, Rio de Janeiro (Brazil). Nucleo de Pesquisas de Produtos Naturais

    1994-12-31

    This work describes the process of analysis of a mixture of polar compounds obtained from the extract of Phyllanthus tennellus, a plant known in Brazil as `quebra-pedra`, which is extensively used in Brazilian popular medicine. The methodology, which includes the use of chromatography and NMR, is described. The proposed composition of the mixture is presented. A discussion is presented concerning one component which could not be identified

  9. Use of the image by nuclear magnetic resonance (nucleography) in obstetrical aspects. Part 2. Emprego da imagem por ressonancia magnetica nuclear (nucleografia) em obstetricia. Parte 2

    Energy Technology Data Exchange (ETDEWEB)

    Lacreta, O [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina

    1987-10-01

    In the first part of this paper the author presented the physical principles on magnetic resonance imaging. The author describes now the obstetrical aspects of nucleography, showing how the maternal and the fetal organs are depicted. The clinical significance of the images is presented. (author).

  10. Use of the image by nuclear magnetic resonance (nucleography) in obstetrical aspects. Part 1. Emprego da imagem por ressonancia magnetica nuclear (nucleografia) em obstetricia. Parte 1

    Energy Technology Data Exchange (ETDEWEB)

    Lacreta, O [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina

    1987-09-01

    Nuclear magnetic resonance (nucleography) is a new method to study human body. In this paper the physical principles on nuclear magnetic resonance and its applications to the pregnant women are presented. (author).

  11. Magnetic resonance imaging identification of muscular and ligamentous structures of the female pelvic floor; Identificacao das estruturas musculo-ligamentares do assoalho pelvico feminino na ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Bezerra, Maria Rita Lima; Soares, Adriano Fleury F.; Faintuch, Salomao; Goldman, Suzan Menasce; Ajzen, Sergio A.; D' Ippolito, Giuseppe; Szejnfeld, Jacob [Universidade Federal de Sao Paulo (UNIFESP/EPM), Sao Paulo, SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: mrcfc@ig.com.br; Girao, Manoel [Universidade Federal de Sao Paulo (UNIFESP/EPM), Sao Paulo, SP (Brazil). Dept. de Ginecologia

    2001-12-01

    The objective was to determine the frequency and to assess the interobserver agreement of identification of muscular and ligamentous pelvic floor structures using magnetic resonance imaging. The method: twenty asymptomatic female volunteers (aged 20-80 years old; mean: 50) were submitted to magnetic resonance imaging (1.5 T) examinations of the pelvis. Turbo spin-echo sequences were employed to obtain T1 and T2 weighted images on axial and sagittal planes. Two independent observers evaluated the scans in order to identify the levator ani (coccygeal, pubococcygeal, iliococcygeal and puborectalis muscles), obturatorius internus and urethral sphincter muscles, and the pubovesical and pubourethral ligaments. The frequency and the interobserver agreement of the identification of the anatomical structures were assessed (kappa statistic - {kappa}). The results: the frequency of identification of the structures ranged from 50 to 100%, and was slightly lower for identification of the ligaments. Interobserver agreement was as follows: levator ani and obturatorius internus muscle ({kappa}=1), pubococcygeal ({kappa}=0.62), iliococcygeal ({kappa}=0.86), puborectalis ({kappa}= 0.27), coccygeal ({kappa}=0) and urethral sphincter muscles ({kappa}=1), pubovesical ({kappa}=0.50) and pubourethral ({kappa}=0.58) ligaments. The conclusion: magnetic resonance imaging of the pelvis allowed precise identification of the main muscular and ligamentous pelvic floor structures in most individuals, whereas interobserver agreement was considered good. (author)

  12. Parenchymal abnormalities in cerebral venous thrombosis: findings of magnetic resonance imaging and magnetic resonance angiography; Alteracoes parenquimatosas na trombose venosa cerebral: aspectos da ressonancia magnetica e da angiorressonancia

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Clecia Santos; Pellini, Marcos [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia]. E-mail: csferreira@superig.com.br; Boasquevisque, Edson [Universidade do Estado do Rio de Janeiro, (UERJ), RJ (Brazil). Faculdade de Medicina. Dept. de Patologia; Souza, Luis Alberto M. de [Hospital da Beneficencia Portuguesa do Rio de Janeiro, RJ (Brazil). Servico de Imagem. Setor de Ressonancia Magnetica

    2006-09-15

    Objective: to determine the frequency and localization of parenchymal abnormalities in cerebral venous thrombosis on magnetic resonance imaging and magnetic resonance angiography as well as their correlation with the territory and affected venous drainage. Materials and methods: retrospective analysis (1996 to 2004) of 21 patients (3 male and 18 female) age range between 3 and 82 years (mean 40 years, median 36 years) with clinical and radiological diagnosis of cerebral venous thrombosis on magnetic resonance imaging and magnetic resonance angiography in 2D PC, 3D PC and contrast-enhanced 3D TOF sequences. The statistical analysis was performed with the qui-square test. Four patients had follow-up exams and three patients underwent digital subtraction angiography. Results: main predisposing factors were: infection, use of oral contraceptives, hormone replacement therapy and collagenosis. Predominant symptoms included: focal deficit, headache, alteration of consciousness level and seizures. Most frequent parenchymal manifestations were: cortical/subcortical edema or infarct, venous congestion and collateral circulation, meningeal enhancement and thalamic and basal ganglia edema or infarct. Occlusion occurred mainly in superior sagittal, left transverse, left sigmoid and straight sinuses. Cavernous sinus and cortical veins thrombosis are uncommon events. Conclusion: cerebral venous thrombosis is an uncommon cause of stroke, with favorable prognosis because of its reversibility. Diagnosis is highly dependent on the radiologist capacity to recognize the presentations of this disease, principally in cases where the diagnosis is suggested by parenchymal abnormalities rather than necessarily by visualization of the thrombus itself. An accurate and rapid diagnosis allows an immediate treatment, reducing the morbidity and mortality rates. (author)

  13. Iron oxide nanoparticles for use in contrast agents in magnetic resonance imaging; Nanoparticulas de oxido de ferro para uso como agentes de contraste em imagens por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Elisa M.N. de; Rocha, Maximiliano S. da; Caimi, Priscila de A.; Basso, Nara R. de S.; Zanini, Mara L.; Papaleo, Ricardo M., E-mail: elisa.oliveira@acad.pucrs.br [Pontificia Universidade Catolica do Rio Grande do Sul (PUC-RS), Porto Alegre, RS (Brazil)

    2015-07-01

    In this work were carried out synthesis of iron oxide nanoparticles coated with dextran, comparing the results of using different concentrations of dextran, iron salts, temperature and reaction time. The compounds were analyzed by DLS, XRD, TGA, TEM, FTIR, Zeta Potential and relaxivity. Nanoparticles with dispersion around 10-15 nm and average hydrodynamic diameters of 16-50 nm, with superparamagnetic behavior were obtained. The ratio of the relaxivities (r2/r1) in aqueous solutions was 5.30, close to value of the commercially available iron oxide contrast agents. (author)

  14. Alzheimer's disease and magnetic resonance spectroscopy of the hippocampus; Doenca de Alzheimer e espectroscopia por ressonancia magnetica do hipocampo

    Energy Technology Data Exchange (ETDEWEB)

    Engelhardt, Eliasz; Moreira, Denise M. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Neurologia Deolindo Couto; Laks, Jerson; Marinho, Valeska M.; Rozenthal, Marcia [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Psiquiatria; Oliveira Junior, Amarino C. [Hospital ProCardiaco, Rio de Janeiro, RJ (Brazil). Setor de Radiologia

    2001-12-01

    Objective: acquisition of data of magnetic resonance metabolite spectrum of the hippocampal formation (hippocampus-hc) in the elderly, normal and with Alzheimer's disease (AD). Method: Subjects matched for age: a. normal sample (n=20), CDR=0, and b. AD sample (n=40), CDR 1 and 2. Technique: Signa Horizon LX-GE, 1.5T, {sup 1}H-MRS with automated software PROBE/SV, VOI: hc (right and left); single voxel (2x2x2cm); TR 1500ms/TE 50ms; PRESS; metabolites: N-acetylaspartate (Naa), choline (Cho), creatine (Cr), myo-inositol (mI). Results: The present data relate to the ratios of Naa, Cho and mI, with Cr taken as reference, and the mI/Naa ratio. The study showed reduction of Naa, increase of mI and of the mI/Naa ratio, and not consistent results for Cho. The results of the whole sample of AD patients compared to the pooled normal mean {+-} sd were significant for Naa, mI and mI/Naa (p<0.01). Accuracy in relation to the individual values of both samples showed satisfactory levels of sensitivity, specificity and positive predictive value. Conclusion: The present results can be used as a helpful tool to detect pathologic changes of the hippocampus in AD, and allowing greater accuracy and an earlier diagnosis of this disease. (author)

  15. Value of magnetic resonance imaging in prenatal diagnosis of placental accretism; Valor da ressonancia magnetica no diagnostico antenatal do acretismo placentario

    Energy Technology Data Exchange (ETDEWEB)

    Francisco, Viviane Vieira; Goldman, Suzan Menasce; Faria, Juliano; Szejnfeld, Jacob [Universidade Federal de Sao Paulo, SP (Brazil). Escola Paulista de Medicina. Dept. de Diagnostico por Imagem]. E-mail: vieira_francisco@uol.com.br

    2006-12-15

    Purpose: to establish the main signs of placental accretism in magnetic resonance imaging (MRI) in patients with clinical suspicion and to estimate the benefit of this method. Methods: prospective transversal study with 15 patients suspected of placental accretism, referred between March 2003 and February 2006. Gestational age varied from 20 to 31 weeks. All patients underwent MRI to study the placenta and had previously done an ultrasonography. Material was sent to histological study. MRI was done on Magnetom Impact and Sonata Maestro Class Siemens{sup R}, with acquired sequences HASTE, TURBO SPIN in axial, sagittal, coronal planes and echo gradient (GE{sup R}), pre- and post-dynamic contrast in the best plan for acquisition. Images were analyzed by a team of two radiologists. Results: mean gestational age was 24.3 weeks. We studied seven placenta previa (47%), six anterior placentas (40%) and two posterior placentas (13%). Ultrasonography was positive in 80% of the placentas and MRI in 53%. However, echography had a low concordance with anatomic pathological studies by Kappa test (11%), revealing 75% of sensitivity, 14% of specificity, 50% as positive predictive value (PPV) and 33% as negative predictive value (NPV). MRI had an excellent concordance with anatomic pathological studies (0.86), showing 100% of sensitivity, 86% of specificity, 89% as PPV and 100% as NPV. Conclusions: MRI is useful for placental accretism diagnosis. The principal findings are transmural hyper-signal, the loss of continuity in myometrial wall in fast sequences and the identification of vessels invading myometrial layer in dynamic sequences. (author)

  16. Ovarian granulocytic sarcoma: a case report and magnetic resonance imaging findings; Sarcoma granulocitico no ovario: relato de caso e achados na ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Licia Pacheco [Hospital Geral de Fortaleza (HGF), CE (Brazil). Servico de Diagnostico por Imagem], e-mail: licia_p@hotmail.com; Silveira, Claudio Regis Sampaio [Hospital Geral de Fortaleza (HGF), CE (Brazil). Servico de Radiologia; Costa, Fabricio da Silva [Universidade Estadual do Ceara (UECE), CE (Brazil); Monte, Hipolito [Hospital Monte Klinikum, Fortaleza, CE (Brazil)

    2008-12-15

    Granulocytic sarcoma (chloroma) is a tumor consisting of myeloid precursors in an extramedullary site. It is complication of both acute and chronic myelogenous leukemias. Although the lesion can occur at any site, ovarian involvement is rare. We report a case of ovary tumor associated with acute myeloid leukaemia and its imaging appearance on magnetic resonance. (author)

  17. Molecular characterization and phylogenetic analysis of Fasciola hepatica from Peru.

    Science.gov (United States)

    Ichikawa-Seki, Madoka; Ortiz, Pedro; Cabrera, Maria; Hobán, Cristian; Itagaki, Tadashi

    2016-06-01

    The causative agent of fasciolosis in South America is thought to be Fasciola hepatica. In this study, Fasciola flukes from Peru were analyzed to investigate their genetic structure and phylogenetic relationships with those from other countries. Fasciola flukes were collected from the three definitive host species: cattle, sheep, and pigs. They were identified as F. hepatica because mature sperms were observed in their seminal vesicles, and also they displayed Fh type, which has an identical fragment pattern to F. hepatica in the nuclear internal transcribed spacer 1. Eight haplotypes were obtained from the mitochondrial NADH dehydrogenase subunit 1 (nad1) sequences of Peruvian F. hepatica; however, no special difference in genetic structure was observed between the three host species. Its extremely low genetic diversity suggests that the Peruvian population was introduced from other regions. Nad1 haplotypes identical to those of Peruvian F. hepatica were detected in China, Uruguay, Italy, Iran, and Australia. Our results indicate that F. hepatica rapidly expanded its range due to human migration. Future studies are required to elucidate dispersal route of F. hepatica from Europe, its probable origin, to other areas, including Peru. Copyright © 2015. Published by Elsevier Ireland Ltd.

  18. BACTERIOLOGICAL INVESTIGATION ON Fasciola hepatica AND CATTLE BILIARY DUCTS

    Directory of Open Access Journals (Sweden)

    A. Panebianco

    2009-03-01

    Full Text Available The aim of this work was to investigate the occurrence of bacteria in Fasciola hepatica and into cattle biliary ducts containing the parasite. A total of 24 liver and 58 F. hepatica samples were analysed. In all biliary ducts and in 62,06% of parasite Enterobacteriaceae were isolated. The bacterial specie more frequently isolated from parasite were Citrobacter freundii (34%, Proteus mirabilis (18%, Providencia rettgeri (12%, Staphylococcus spp. (18%, Enterobacter spp. (12%. There doesn’t appear to be a correlation between bacterial specie from parasite and ducts. The Authors conclude with some related inspective consideration.

  19. Topography and distribution of ostia venae hepatica in the ...

    African Journals Online (AJOL)

    BACKGROUND: Openings of hepatic veins into the retrohepatic surface of the inferior vena cava. (ostia venae hepatica) play a part in controlling hepatic circulation by acting as collateral channels in obstruction. Their topography and distribution must be taken into account during catheterization and liver transplantation.

  20. Sheep and Fasciola hepatica in Europe: the GLOWORM experience

    Directory of Open Access Journals (Sweden)

    Laura Rinaldi

    2015-03-01

    Full Text Available Fasciola hepatica infection challenges health, welfare and productivity of small ruminants throughout the world. The distribution of F. hepatica in sheep in Europe is usually scattered and studies are generally concerned with a single area making it difficult to compare results from different environments, climates and management regimes. In order to elucidate the current scenario in terms of prevalence and intensity of F. hepatica infection in sheep farms across Europe, a standardized cross-sectional survey was conducted in three pilot areas in Ireland, Switzerland and Italy, all part of the EU funded GLOWORM project. Two consecutive field surveys (in 2012 and 2013 were conducted in the three countries in the same period (August-October in 361 sheep farms in total. Harmonized procedures (from farm to laboratory based on pooled samples and the highly sensitive and accurate, diagnostic FLOTAC technique were used. The georeferenced parasitological results were modelled (at the pilot area level following a Bayesian geostatistical approach with correction for preferential sampling and accounting for climatic and environmental covariates. The observed F. hepatica prevalence rates did not differ between the two study years in any of the three pilot areas, but they did vary between the countries showing high values in Ireland (61.6% compared to Italy (7.9% and Switzerland (4.0%. Spatial patterns of F. hepatica distribution were detected by the Bayesian geostatistical approach in Ireland with a high risk of infection in the south-western part of the pilot area there. The latent factor analysis highlighted the importance of year-to-year variation of mean temperature, rainfall and seasonality within a country, while long-term trends of temperature and rainfall dominated between countries with respect to prevalence of infection.

  1. Fasciola hepatica Infection in an Indigenous Community of the Peruvian Jungle.

    Science.gov (United States)

    Cabada, Miguel M; Castellanos-Gonzalez, Alejandro; Lopez, Martha; Caravedo, María Alejandra; Arque, Eulogia; White, Arthur Clinton

    2016-06-01

    Fasciola hepatica is a zoonotic infection with a worldwide distribution. Autochthonous cases have not been reported in the Amazon region of Peru. Operculated eggs resembling F. hepatica were identified in the stools of five out of 215 subjects in a remote indigenous community of the Peruvian jungle. Polymerase chain reaction targeting Fasciola hepatica cytochrome oxidase subunit 1 (COI) gene and sequencing of the products confirmed Fasciola infection. © The American Society of Tropical Medicine and Hygiene.

  2. Fasciola hepatica Infection in an Indigenous Community of the Peruvian Jungle

    OpenAIRE

    Cabada, Miguel M.; Castellanos-Gonzalez, Alejandro; Lopez, Martha; Caravedo, Mar��a Alejandra; Arque, Eulogia; White, Arthur Clinton

    2016-01-01

    Fasciola hepatica is a zoonotic infection with a worldwide distribution. Autochthonous cases have not been reported in the Amazon region of Peru. Operculated eggs resembling F. hepatica were identified in the stools of five out of 215 subjects in a remote indigenous community of the Peruvian jungle. Polymerase chain reaction targeting Fasciola hepatica cytochrome oxidase subunit 1 (COI) gene and sequencing of the products confirmed Fasciola infection.

  3. Magnetic nanoparticles for cancer therapy; Nanoparticulas magneticas para o tratamento do cancer

    Energy Technology Data Exchange (ETDEWEB)

    Bakuzis, Andris F. [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Instituto de Fisica

    2014-07-01

    Full text: Magnetic nanoparticles have been used in several biomedical applications, spanning from cell separation, early diagnosis of metastasis to even the treatment of cancer via magnetic hyperthermia (MH). This last technique consists in the increase of temperature of nanoparticles when their magnetic moments interact with a magnetic alternating field. This effect has been suggested as an innovative therapy to cancer treatment, due to the delivery of heat or therapeutic agents, such as drugs, genes, and others. In addition, several clinical studies has demonstrated synergetic effects between hyperthermia and radiotherapy [1]. This indicates a great therapeutic potential for this noninvasive and targeted technique. In this talk we will discuss results from the literature and from our own group in the treatment of cancer via magnetic hyperthermia. Several types of magnetic nanoparticles suggested for this application will be discussed, as well as the historical evolution of this procedure, which although suggested in the late 50' only recently was approved in Europe for treatment of humans with brain tumors. (author) [Portuguese] Full text: Nanoparticulas magneticas tem sido utilizadas em diversas aplicacoes biomedicas, desde a separacao de celulas, marcacao de celulas-tronco, diagnostico precoce de metastases ao tratamento do cancer via hipertermia magnetica (HM). Esta ultima tecnica consiste no aumento da temperatura de nanoparticulas quando seus momentos magneticos interagem de forma adequada com um campo magnetico alternado. Este efeito tem sido idealizado como uma nova terapia para o tratamento do cancro, seja via entrega seletiva de calor ou mesmo pela liberacao de farmacos, genes ou outros agentes terapeuticos por meio desta acao externa. Adicionalmente, diversos estudos clinicos tem demonstrado efeitos sinergicos entre a hipertermia e tecnicas tradicionais de tratamento oncologico, como quimioterapia e radioterapia. Isto indica um forte potencial

  4. Fasciola hepatica from naturally infected sheep and cattle in Great Britain are diploid.

    Science.gov (United States)

    Beesley, N J; Cwiklinski, K; Williams, D J L; Hodgkinson, J

    2015-08-01

    Diploid (2n = 2x = 20) and triploid (2n = 3x = 30) Fasciola hepatica have been reported in the UK, and in Asia diploid, triploid and mixoploid (2x/3x) Fasciola spp. exist but there is little information to indicate how common triploidy is, particularly in UK fluke. Here the ploidy of 565 adult F. hepatica from 66 naturally infected British sheep and 150 adult F. hepatica from 35 naturally infected British cattle was determined. All 715 of these parasites were diploid, based on observation of 10 bivalent chromosomes and sperm (n = 335) or, since triploids are aspermic, sperm alone (n = 380). This constitutes the first extensive analysis of the ploidy of F. hepatica field isolates from Great Britain and shows that most F. hepatica isolated from cattle and sheep are diploid and have the capacity to sexually reproduce. These data suggest that triploidy, and by extension parthenogenesis, is rare or non-existent in wild British F. hepatica populations. Given that F. hepatica is the only species of Fasciola present in Britain our results indicate that the parasite is predominantly diploid in areas where F. hepatica exists in isolation and suggests that triploidy may only originate in natural populations where co-infection of F. hepatica and its sister species Fasciola gigantica commonly occurs.

  5. Transcriptomic Study on Ovine Immune Responses to Fasciola hepatica Infection.

    Directory of Open Access Journals (Sweden)

    Yan Fu

    2016-09-01

    Full Text Available Fasciola hepatica is not only responsible for major economic losses in livestock farming, but is also a major food-borne zoonotic agent, with 180 million people being at risk of infection worldwide. This parasite is sophisticated in manipulating the hosts' immune system to benefit its own survival. A better understanding of the mechanisms underpinning this immunomodulation is crucial for the development of control strategies such as vaccines.This in vivo study investigated the global gene expression changes of ovine peripheral blood mononuclear cells (PBMC response to both acute & chronic infection of F. hepatica, and revealed 6490 and 2364 differential expressed genes (DEGS, respectively. Several transcriptional regulators were predicted to be significantly inhibited (e.g. IL12 and IL18 or activated (e.g. miR155-5p in PBMC during infection. Ingenuity Pathway Analysis highlighted a series of immune-associated pathways involved in the response to infection, including 'Transforming Growth Factor Beta (TGFβ signaling', 'Production of Nitric Oxide in Macrophages', 'Toll-like Receptor (TLRs Signaling', 'Death Receptor Signaling' and 'IL17 Signaling'. We hypothesize that activation of pathways relevant to fibrosis in ovine chronic infection, may differ from those seen in cattle. Potential mechanisms behind immunomodulation in F. hepatica infection are a discussed.In conclusion, the present study performed global transcriptomic analysis of ovine PBMC, the primary innate/adaptive immune cells, in response to infection with F. hepatica, using deep-sequencing (RNAseq. This dataset provides novel information pertinent to understanding of the pathological processes in fasciolosis, as well as a base from which to further refine development of vaccines.

  6. Retirada de fasciola hepatica da via biliar principal por coledocoscopia

    OpenAIRE

    Coral, Roberto Pelegrini; Mastalir, Eduardo T.; Mastalir, Fabiane Pinto

    2007-01-01

    The authors present a rare case of hepatic fascioliasis in a female patient 53-years-old, coming from the rural zone of Rio Grande do Sul, a southern State from Brazil. She has presented with biliary colic, fluctuant jaundice and eosinophilia. Abdominal ultrasound has shown a dilated biliary tree with inside heterogeneous images. At surgery we have found inside the biliary tree several Fasciola hepatica, which have been pulled out with the choledocoscope. We have proceeded with biliodigestive...

  7. Controversial aspects of the life cycle of Fasciola hepatica.

    Science.gov (United States)

    Moazeni, Mohammad; Ahmadi, Amin

    2016-10-01

    Fasciola hepatica is a well-known helminth parasite, with significant economic and public health importance all over the world. It has been known since more than 630 years ago and a considerable research work has been carried out on the life cycle of this important parasite. In the hepatic phase of the life cycle of F. hepatica, it is assumed that the young flukes, after about 6-7 weeks of migration in the liver parenchyma, enter into the bile ducts of the definitive hosts and become sexually mature. Even though the secretion of cysteine peptidases including cathepsin L and B proteases by F. hepatica may justify this opinion, because of several scientific reasons and based on the experimental studies conducted in different animals (reviewed in this article), the entry of parasites into the bile ducts, after their migration in the liver parenchyma seems to be doubtful. However, considering all the facts relating to the hepatic and biliary phases of the life cycle of F. hepatica, two alternative ideas are suggested: 1) some of the migrating juvenile flukes may enter into the bile ducts immediately after reaching the liver parenchyma while they are still very small, or 2) when newly excysted juvenile flukes are penetrating into the intestinal wall to reach the liver through the abdominal cavity, a number of these flukes may enter into the choleduct and reach the hepatic bile ducts, where they mature. According to the previously performed natural and experimental studies in different animals and human beings, the supporting and opposing evidences for the current opinion as well as the evidences that might justify the two new ideas are reviewed and discussed briefly. In conclusion, our present knowledge about the time and quality of the entry of F. hepaticas into the bile ducts, seems to be insufficient, therefore, there are still some dark corners and unknown aspects in this field that should be clarified. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea.

    Science.gov (United States)

    Lee, Jae-Hyung; Quan, Juan-Hua; Choi, In-Wook; Park, Gab-Man; Cha, Guang-Ho; Kim, Hyun-Ju; Yuk, Jae-Min; Lee, Young-Ha

    2017-02-01

    Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 402 samples, F. hepatica 1TS-2 marker was detected in 6 freshwater snails; thus, the overall prevalence in freshwater snails was 1.5%. The prevalence varied between collection areas, ranging from 0.0% at Hoenggye-ri to 2.9% at Suha-ri. However, F. gigantica ITS-2 was not detected in the 6 F. hepatica -positive samples by PCR. The nucleotide sequences of the 6 F. hepatica ITS-2 PCR-positive samples were 99.4% identical to the F. hepatica ITS-2 sequences in GenBank, whereas they were 98.4% similar to F. gigantica ITS-2 sequences. These results indicated that the prevalence of F. hepatica in snail intermediate hosts was 1.5% in Gangwon-do, Korea; however the prevalence varied between collection areas. These results may help us to understand F. hepatica infection status in natural environments.

  9. Fasciola hepatica in snails collected from water-dropwort fields using PCR.

    Science.gov (United States)

    Kim, Hwang-Yong; Choi, In-Wook; Kim, Yeon-Rok; Quan, Juan-Hua; Ismail, Hassan Ahmed Hassan Ahmed; Cha, Guang-Ho; Hong, Sung-Jong; Lee, Young-Ha

    2014-12-01

    Fasciola hepatica is a trematode that causes zoonosis mainly in cattle and sheep and occasionally in humans. Fascioliasis has been reported in Korea; however, determining F. hepatica infection in snails has not been done recently. Thus, using PCR, we evaluated the prevalence of F. hepatica infection in snails at 4 large water-dropwort fields. Among 349 examined snails, F. hepatica-specific internal transcribed space 1 (ITS-1) and/or ITS-2 markers were detected in 12 snails and confirmed using sequence analysis. Morphologically, 213 of 349 collected snails were dextral shelled, which is the same aperture as the lymnaeid snail, the vectorial host for F. hepatica. Among the 12 F. hepatica-infected snails, 6 were known first intermediate hosts in Korea (Lymnaea viridis and L. ollula) and the remaining 6 (Lymnaea sp.) were potentially a new first intermediate host in Korea. It has been shown that the overall prevalence of the snails contaminated with F. hepatica in water-dropwort fields was 3.4%; however, the prevalence varied among the fields. This is the first study to estimate the prevalence of F. hepatica infection using the vectorial capacity of the snails in Korea.

  10. Retirada de fasciola hepatica da via biliar principal por coledocoscopia

    Directory of Open Access Journals (Sweden)

    Roberto Pelegrini Coral

    Full Text Available The authors present a rare case of hepatic fascioliasis in a female patient 53-years-old, coming from the rural zone of Rio Grande do Sul, a southern State from Brazil. She has presented with biliary colic, fluctuant jaundice and eosinophilia. Abdominal ultrasound has shown a dilated biliary tree with inside heterogeneous images. At surgery we have found inside the biliary tree several Fasciola hepatica, which have been pulled out with the choledocoscope. We have proceeded with biliodigestive anastomosis using the small intestine. The patient remains asymptomatic six months after surgical procedure. Small intestine. The patient remains asymptomatic six months after surgical procedure.

  11. Immune responses induced by co-infection with Capillaria hepatica in Clonorchis sinensis-infected rats.

    Science.gov (United States)

    Moon, E-K; Lee, S-H; Goo, T W; Quan, F-S

    2018-07-01

    Clonorchis sinensis and Capillaria hepatica are zoonotic parasites that mainly infect the liver and cause serious liver disorders. However, immunological parameters induced by co-infection with these parasites remain unknown. In this study, for the first time, we investigated immunological profiles induced by co-infection with C. hepatica (CH) in C. sinensis (CS)-infected rats (Sprague-Dawley). Rats were infected primarily with 50 metacercariae of C. sinensis; 4 weeks later, they were subsequently infected with 1000 infective C. hepatica eggs. Significantly higher levels of C. sinensis- or C. hepatica-specific IgG antibodies were found in the sera of rats. Interestingly, no cross-reacting antibody was observed between C. sinensis and C. hepatica infections. Significantly raised eosinophil levels were found in the blood of C. sinensis/C. hepatica co-infected rats (CS + CH) compared to the blood of rats infected singly with C. sinensis. Co-infected rats showed significantly higher levels of lymphocyte proliferation and cytokine production compared to a single C. sinensis infection. The worm burden of C. sinensis was significantly reduced in co-infected rats compared to the single C. sinensis infection. These results indicate that the eosinophils, lymphocyte proliferation and cytokine production induced by subsequent infection with C. hepatica in C. sinensis-infected rats might contribute to the observed C. sinensis worm reduction.

  12. Effect of gamma irradiation on different stages of Fasciola hepatica

    Energy Technology Data Exchange (ETDEWEB)

    Torres, J.L.; Chiriboga, J.

    1976-04-01

    Fascioliasis or hepatic distomatosis is one of the most widespread liver diseases throughout the world. It is caused by a parasitic worm of the class Trematoda of the phylum Platyhelminthes. This flatworm is commonly known as liver-fluke in the United States and Europe; babosa del higado in Cuba; cucaracha del higado in Puerto Rico; saguaype in Argentina and Chile; pirihuin in Chile; yuta in North Chile, and it is taxonomically classified as Fasciola hepatica. The adult trematode is a common parasite of warm blooded animals especially cattle, sheep, and goats. Alterations in the viability of normal Fasciola hepatica metacercariae as a result of time of storage at 4/sup 0/C were studied by the method of Wikerhauser (1960). A significant decrease in viability was observed only after one hundred days of storage. The in vitro viability of normal metacercariae was compared with that of metacercariae that were exposed to increasing ..gamma.. radiation doses. Two plainly opposite effects observed were: the activation of the excystation process by the lower radiation doses (1.5 and 2.5 Kr), an effect that persisted up to the 14th day post-radiation, and the impairment of the process evidenced on the 14th day in the metacercariae exposed to 3.5 and 5.0 Kr. Correlation between liver damage and the increasing radiation doses to which the metacercariae were exposed was studied up to 34 days after the oral inoculation in rats. (auth)

  13. Effect of gamma irradiation on different stages of Fasciola hepatica

    International Nuclear Information System (INIS)

    Torres, J.L.; Chiriboga, J.

    1976-04-01

    Fascioliasis or hepatic distomatosis is one of the most widespread liver diseases throughout the world. It is caused by a parasitic worm of the class Trematoda of the phylum Platyhelminthes. This flatworm is commonly known as liver-fluke in the United States and Europe; babosa del higado in Cuba; cucaracha del higado in Puerto Rico; saguaype in Argentina and Chile; pirihuin in Chile; yuta in North Chile, and it is taxonomically classified as Fasciola hepatica. The adult trematode is a common parasite of warm blooded animals especially cattle, sheep, and goats. Alterations in the viability of normal Fasciola hepatica metacercariae as a result of time of storage at 4 0 C were studied by the method of Wikerhauser (1960). A significant decrease in viability was observed only after one hundred days of storage. The in vitro viability of normal metacercariae was compared with that of metacercariae that were exposed to increasing γ radiation doses. Two plainly opposite effects observed were: the activation of the excystation process by the lower radiation doses (1.5 and 2.5 Kr), an effect that persisted up to the 14th day post-radiation, and the impairment of the process evidenced on the 14th day in the metacercariae exposed to 3.5 and 5.0 Kr. Correlation between liver damage and the increasing radiation doses to which the metacercariae were exposed was studied up to 34 days after the oral inoculation in rats

  14. Three Living Fasciola Hepatica in the Biliary Tract of a Woman

    Science.gov (United States)

    Niknam, Ramin; Kazemi, Mohammad Hassan; Mahmoudi, Laleh

    2015-01-01

    Fasciola hepatica (F. hepatica) as a foodborne trematode can occasionally cause hepatobiliary diseases. We report a 67-year-old woman who was referred to our center because of the diagnosis of cholangitis. She was a resident of mountainous area with the history of unsafe water and contaminated vegetables. Endoscopic retrograde cholangiopancreatography (ERCP) was performed as a diagnostic and therapeutic modality for her. Three living F. hepatica was removed from biliary tract with a basket via ERCP. Clinical and laboratory condition of the patient improved after therapy of antibiotics and triclabendazole. PMID:26379355

  15. Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis

    Science.gov (United States)

    Kim, Kwangsig; Yang, Hyun Jong

    2003-01-01

    This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis. PMID:12815325

  16. Ectopic Human Fasciola hepatica Infection by an Adult Worm in the Mesocolon.

    Science.gov (United States)

    Kim, Ah Jin; Choi, Chang Hwan; Choi, Sun Keun; Shin, Yong Woon; Park, Yun-Kyu; Kim, Lucia; Choi, Suk Jin; Han, Jee Young; Kim, Joon Mee; Chu, Young Chae; Park, In Suh

    2015-12-01

    We report here an ectopic case of Fasciola hepatica infection confirmed by recovery of an adult worm in the mesocolon. A 56-year-old female was admitted to our hospital with discomfort and pain in the left lower quadrant of the abdomen. Abdominal CT showed 3 abscesses in the left upper quadrant, mesentery, and pelvic cavity. On surgical exploration, abscess pockets were found in the mesocolon of the sigmoid colon and transverse colon. A leaf-like worm found in the abscess pocket of the mesocolon of the left colon was diagnosed as an adult fluke of F. hepatica. Histologically, numerous eggs of F. hepatica were noted with acute and chronic granulomatous inflammations in the subserosa and pericolic adipose tissues. Conclusively, a rare case of ectopic fascioliasis has been confirmed in this study by the adult worm recovery of F. hepatica in the mesocolon.

  17. Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR

    Directory of Open Access Journals (Sweden)

    Kelly Grace Magalhães

    2004-06-01

    Full Text Available From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.

  18. Fasciola hepatica infestation as a very rare cause of extrahepatic cholestasis

    Institute of Scientific and Technical Information of China (English)

    Ahmet Dobrucali; Rafet Yigitbasi; Yusuf Erzin; Oguzhan Sunamak; Erdal Polat; Hakan Yakar

    2004-01-01

    Fasciola hepatica, an endemic parasite in Turkey, is still a very rare cause of cholestasis worldwide. Through ingestion of contaminated water plants like watercress, humans can become the definitive host of this parasite. Cholestatic symptoms may be sudden but in some cases they may be preceeded by a long period of fever, eosinophilia and vague gastrointestinal symptoms. We report a woman with cholangitis symptoms of sudden onset which was proved to be due to Fasciola hepatica infestation by an endoscopic retrograde cholangiography.

  19. Epidemiology and impact of Fasciola hepatica exposure in high-yielding dairy herds.

    Science.gov (United States)

    Howell, Alison; Baylis, Matthew; Smith, Rob; Pinchbeck, Gina; Williams, Diana

    2015-09-01

    The liver fluke Fasciola hepatica is a trematode parasite with a worldwide distribution and is the cause of important production losses in the dairy industry. The aim of this observational study was to assess the prevalence of exposure to F. hepatica in a group of high yielding dairy herds, to determine the risk factors and investigate their associations with production and fertility parameters. Bulk milk tank samples from 606 herds that supply a single retailer with liquid milk were tested with an antibody ELISA for F. hepatica. Multivariable linear regression was used to investigate the effect of farm management and environmental risk factors on F. hepatica exposure. Higher rainfall, grazing boggy pasture, presence of beef cattle on farm, access to a stream or pond and smaller herd size were associated with an increased risk of exposure. Univariable regression was used to look for associations between fluke exposure and production-related variables including milk yield, composition, somatic cell count and calving index. Although causation cannot be assumed, a significant (phepatica exposure and estimated milk yield at the herd level, representing a 15% decrease in yield for an increase in F. hepatica exposure from the 25th to the 75th percentile. This remained significant when fertility, farm management and environmental factors were controlled for. No associations were found between F. hepatica exposure and any of the other production, disease or fertility variables. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  20. Surgical neuro navigator guided by preoperative magnetic resonance images, based on a magnetic position sensor;Neuronavegador cirurgico guiado por imagens de ressonancia magnetica pre-operatoria, baseado num transdutor de posicao magnetico

    Energy Technology Data Exchange (ETDEWEB)

    Perini, Ana Paula; Siqueira, Rogerio Bulha; Carneiro, Antonio Adilton Oliveira, E-mail: adilton@ffclrp.usp.b [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Filosofia, Ciencias e Letras. Dept. de Fisica e Matematica; Oliveira, Lucas Ferrari de [Universidade Federal de Pelotas (UFPel), RS (Brazil). Dept. de Informatica; Machado, Helio Rubens [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Dept. de Neurocirurgia

    2009-08-15

    Image guided neurosurgery enables the neurosurgeon to navigate inside the patient's brain using pre-operative images as a guide and a tracking system, during a surgery. Following a calibration procedure, three-dimensional position and orientation of surgical instruments may be transmitted to computer. The spatial information is used to access a region of interest, in the pre-operative images, displaying them to the neurosurgeon during the surgical procedure. However, when a craniotomy is involved and the lesion is removed, movements of brain tissue can be a significant source of error in these conventional navigation systems. The architecture implemented in this work intends the development of a system to surgical planning and orientation guided by ultrasound image. For surgical orientation, the software developed allows the extraction of slices from the volume of the magnetic resonance images (MRI) with orientation supplied by a magnetic position sensor (Polhemus{sup R}). The slices extracted with this software are important because they show the cerebral area that the neurosurgeon is observing during the surgery, and besides they can be correlated with the intra-operative ultrasound images to detect and to correct the deformation of brain tissue during the surgery. Also, a tool for per-operative navigation was developed, providing three orthogonal planes through the image volume. In the methodology used for the software implementation, the Python{sup tm} programming language and the Visualization Toolkit (VTK) graphics library were used. The program to extract slices of the MRI volume allowed the application of transformations in the volume, using coordinates supplied by the position sensor. (author)

  1. Effects of JPEG data compression on magnetic resonance imaging evaluation of small vessels ischemic lesions of the brain; Efeitos da compressao de dados JPEG na avaliacao de lesoes vasculares cerebrais isquemicas de pequenos vasos em ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Kuriki, Paulo Eduardo de Aguiar; Abdala, Nitamar; Nogueira, Roberto Gomes; Carrete Junior, Henrique; Szejnfeld, Jacob [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: paulokuriki@gmail.com

    2006-01-15

    Objective: to establish the maximum achievable JPEG compression ratio without affecting quantitative and qualitative magnetic resonance imaging analysis of ischemic lesion in small vessels of the brain. Material and method: fifteen DICOM images were converted to JPEG with a compression ratio of 1:10 to 1:60 and were assessed together with the original images by three neuro radiologists. The number, morphology and signal intensity of the lesions were analyzed. Results: lesions were properly identified up to a 1:30 ratio. More lesions were identified with a 1:10 ratio then in the original images. Morphology and edges were properly evaluated up toa 1:40 ratio. Compression did not affect signal. Conclusion: small lesions were identified ( < 2 mm ) and in all compression ratios the JPEG algorithm generated image noise that misled observers to identify more lesions in JPEG images then in DICOM images, thus generating false-positive results.(author)

  2. A system dedicated to the viewing and handling of tomographic images obtained by magnetic resonance; Um sistema dedicado a visualizacao e manipulacao de imagens tomograficas obtidas por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Slaets, Joan F.W.; Almeida, Lirio O.B. [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Fisica e Quimica; Traina, Agma J.M. [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Ciencias Matematicas

    1992-12-31

    The present work describes the development of a dedicated system to be used in visualization and manipulation of a MR images. The graphics environment as well as the tool kit were developed for the dedicated TMS34010 based hardware. The developed software offers a compact kernel with primitives to support the creation and manipulation windows and menus directly in `C` language. This work is fundamental for the implementation of a user friendly interface build to operate and visualize tomographic images. This tools are essential for the selection an archiving of images planes as used in clinical applications. (author) 9 refs., 2 figs., 1 photo; e-mail: jan, agma and lirio at uspfsc.ifqsc..usp.ansp.br

  3. Dysgenesis of the corpus callosum and associated malformaaation{sup :} computed tomography and magnetic resonance imaging findings; Disgenesia do corpo caloso e mas-formacoes associadas: achados de tomografia computadorizada e ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Montandon, Cristiano; Montandon Junior, Marcelo Eustaquio [Colegio Brasileiro de Radiologia e Diagnostico por Imagem (CBR), Sao Paulo, SP (Brazil); Ribeiro, Flavia Aparecida de Sousa; Lobo, Leonardo Valadares Barbosa; Teixeira, Kim-Ir-Sen Santos [Goias Univ., Goiania (Brazil). Hospital de Clinicas. Dept. de Diagnostico por Imagem e Anatomia Patologica]. E-mail: cabeca2@terra.com.br

    2003-10-01

    Callosal dysgenesis is a malformation of the corpus callosum with origins in the embryogenesis of the telencephalon. We reviewed the computed tomography and magnetic resonance imaging findings of 11 patients with callosal dysgenesis and one patient with normal corpus callosum associated with a lipoma. The patients were divided into three distinct groups: total agenesis (three patients), partial agenesis (six patients) and hypoplasia (two patients). Associated abnormalities were observed in nine patients, including Chiari II malformation (one patient), schizencephaly (one patient), interhemispheric cyst (two patients), Dandy-Walker cyst (one patient), nodular heterotopy (one patient) and lipoma of the corpus callosum (four patients). This paper presents a review that may contribute to the diagnosis of these disorders. (author)

  4. Preparation of EVA/silica nano composites characterized with solid state nuclear magnetic resonance; Obtencao de nanocomposito de EVA/SILICA e caracterizacao por ressonancia magnetica nuclear no estado solido

    Energy Technology Data Exchange (ETDEWEB)

    Passos, Adriano A; Tavares, Maria I.B.; Neto, Roberto C.P.; Moreira, Leonardo A; Ferreira, Antonio G., E-mail: mibt@ima.ufrj.br [Centro de Tecnologia, Instituto de Macromoleculas Professora Eloisa Mano, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ (Brazil)

    2011-07-01

    Nano composites of poly(ethylene-co-vinyl acetate)/silica (SiO{sub 2}) with dimensions of ca. 40 nm were prepared via solution intercalation employing chloroform as a solvent. They were mainly characterized with nuclear magnetic resonance spectroscopy (NMR) employing carbon-13 (polymeric matrix), silicon-29 (nanoparticles) and through the determination of the proton spin-lattice relaxation time in the rotating frame (T{sub 1{rho}H}) (polymer matrix). From the NMR results it was inferred that up to 5% of silica in mass a well dispersed nano composite was obtained, owing to a strong interaction between silica and the EVA matrix. (author)

  5. Nuclear magnetic resonance system with continuous flow of polarized water to obtain the traceability to static magnetic fields; Sistema de ressonancia magnetica nuclear com fluxo continuo de agua polarizada para obtencao da rastreabilidade para campos magneticos estaticos

    Energy Technology Data Exchange (ETDEWEB)

    Martin, Ramon Valls; Nazarre, Diego Joriro, E-mail: ramon@ipt.br [Instituto de Pesquisas Tecnologicas (IPT), Sao Paulo, SP (Brazil)

    2013-07-01

    We have developed a system to obtain the traceability of field or magnetic induction intensity in the range of 2 μT up to 2 T, even in the presence of magnetic field gradients or noisy environments. The system is based on a nuclear magnetic resonance magnetometer, built in streaming water. The calibration procedure of a coil for magnetic field generation is described, as well as the results obtained and the estimated uncertainty (author)

  6. Characterization of polymer-type ionic conductors using nuclear magnetic resonance and thermal analysis. Humidity sensor; Caracterizacao de condutores ionicos de tipo polimerico por ressonancia magnetica nuclear (RMN) e analises termicas. Sensor de umidade

    Energy Technology Data Exchange (ETDEWEB)

    Cavalcante, Maria Goretti

    1992-04-01

    We report a study using Nuclear Magnetic Resonance (NMR), Thermogravimetry Analysis, Differential Scanning Calorimetry and Infrared Spectroscopy in polymeric complexes formed poly(ethylene oxide), (PEO), and lithium salts. These complexes have have shown a large potential for technological applications in batteries, sensors, etc. We developed and characterized humidity sensors and discussed how the humidity affects the conformation of the complexes, the mobility of ionic species, and the polymeric chains. The results indicate that the hydration affects the conformation of polymeric complexes by plasticizing the water, which induces a volumetric expansion in the PEO chain. The processes was completely reversible for the level of hydration studied. NMR was used to distinguish the movement of polymeric chains from the movement of the ionic species. From the analysis of the second moment of resonance lines from the study of the nuclear relaxation we were able to estimate the average distance between the ionic species and the proton in the complexes chains. The behaviour of spin -lattice relaxation of hydrogen and fluorine in the P(EO) - Li B F, as a function of temperature and frequency reflects the nature of the disorder and the complexity of the ionic conduction process in these materials. (author). 91 refs., 69 figs., 2 tabs.

  7. A system dedicated to the viewing and handling of tomographic images obtained by magnetic resonance; Um sistema dedicado a visualizacao e manipulacao de imagens tomograficas obtidas por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Slaets, Joan F.W.; Almeida, Lirio O.B. [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Fisica e Quimica; Traina, Agma J.M. [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Ciencias Matematicas

    1993-12-31

    The present work describes the development of a dedicated system to be used in visualization and manipulation of a MR images. The graphics environment as well as the tool kit were developed for the dedicated TMS34010 based hardware. The developed software offers a compact kernel with primitives to support the creation and manipulation windows and menus directly in `C` language. This work is fundamental for the implementation of a user friendly interface build to operate and visualize tomographic images. This tools are essential for the selection an archiving of images planes as used in clinical applications. (author) 9 refs., 2 figs., 1 photo; e-mail: jan, agma and lirio at uspfsc.ifqsc..usp.ansp.br

  8. Study of proton and carbon 13 nuclear magnetic resonance spectra of an allyl phenol derivatives series; Estudo de espectros de ressonancia magnetica nuclear protonica e de carbono 13 de uma serie de derivados de alilfenois

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Thais Horta Alvares da [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Faculdade de Farmacia. Dept. de Produtos Farmaceuticos; Oliveira, Alaide Braga de [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Quimica

    1991-12-31

    Quaternary ammonium salt derivates of natural ally phenols were synthesized with the purpose of obtaining peripheral analgesics. Eugenol, O-methyleugenol and safrole were submitted to nitration, reduction, per methylation. The amines were also transformed into their hydro chlorides. The {sup 1} HNMR spectra of the nitro compounds, amines, amine hydro chlorides, quaternary ammonium salts and dymethylamines were analysed, as well as the {sup 13} C NMR spectra of the nitro compounds and of the quaternary ammonium salts. (author). 8 refs., 4 tabs.

  9. Analysis of oil content in sunflower seeds with nuclear magnetic ressonance and `SOXHLET` methods; Determinacao do teor de oleo em sementes de girassol pelos metodos de ressonancia magnetica nuclear e soxhlet

    Energy Technology Data Exchange (ETDEWEB)

    Ungaro, Maria Regina Goncalves [Instituto Agronomico de Campinas, SP (Brazil). Secao de Oleaginosas; Toledo, Nilva Maria Prestes de [Instituto Agronomico de Campinas, SP (Brazil). Secao Tecnica Experimental e Calculo; Teixeira, Joao Paulo Feijao [Instituto Agronomico de Campinas, SP (Brazil). Secao de Fitoquimica; Suassuna Filho, Jose [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Dept.de Fisica

    1993-12-31

    Individual and group of seeds of some sunflower populations were utilized in order to evaluate the feasibility of oil content evaluations by the Nuclear Magnetic Resonance method (NMR). Correlation analysis with the extractive method (Soxhlet) was performed. The results showed that: the oil in the seeds behaved like a liquid in the NMR analysis; there was a high and positive correlation (r = 0,93) between NMR and Soxhlet; there was no correlation between seed weight and oil content; the observed variability in oil content among seeds of a plant were higher than between plants of a population; these values indicate the importance of making individual analysis of oil content by the NMR method for the sunflower breeding for oil content. (author) 7 refs., 2 figs., 4 tabs.

  10. ({sup 1} H, {sup 13} C and {sup 31} P) NMR of phosphonic acid derivatives; Ressonancia magnetica nuclear ({sup 1} H, {sup 13} C, {sup 31} P) de derivados do acido fosfonico

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Valdevino; Costa, Valentim E. Uberti [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Inst. de Quimica

    1992-12-31

    In the last years the development of phosphates analogues in the medical and agricultural pesticides has being very expressive. {sup 1} H, {sup 13} C and mainly {sup 31} P NMR are used for stereochemical and conformational analysis, and reactivity studies on the compounds resulting from those chemical processes 2 refs., 4 figs., 1 tab.

  11. Peritendinous calcinosis of calcaneus tendon associated with dermatomyositis: correlation between conventional radiograph, ultrasound, magnetic resonance imaging and gross surgical pathology; Calcinose peritendinea do tendao calcaneo associada a dermatomiosite: correlacao entre radiografia convencional, ultra-sonografia, ressonancia magnetica e macroscopia cirurgica

    Energy Technology Data Exchange (ETDEWEB)

    Rosa, Ana Claudia Ferreira; Gomide, Lidyane Marques de Paula; Lemes, Marcella Stival [Universidade Federal de Goias (UFG), Goiana, GO (Brazil). Faculdade de Medicina. Hospital das Clinicas; Costa, Edegmar Nunes; Rocha, Valney Luiz da [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Faculdade de Medicina. Dept. de Ortopedia; Machado, Marcio Martins; Santos Junior, Rubens Carneiro dos; Barros, Nestor de; Cerri, Giovanni Guido [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Faculdade de Medicina. Dept. de Radiologia; Sernik, Renato Antonio [Sao Paulo Univ., SP (Brazil). Hospital das Clinicas. Inst. de Radiologia; Nunes, Rodrigo Alvarenga [Universidade do Vale do Sapucai (UNIVAS), Pouso Alegre, MG (Brazil). Faculdade de Ciencias Medicas; Albieri, Alexandre Daher [Hospital de Acidentados de Goiania, GO (Brazil)

    2006-01-15

    Interstitial calcinosis is an uncommon condition in which there is either localized or widely disseminated deposition of calcium in the skin, subcutaneous tissues, muscles, and tendons. Calcinosis is often associated with collagen diseases, scleroderma and dermatomyositis. The authors report a case of interstitial calcinosis associated with dermatomyositis studied with conventional radiograph, ultrasound and magnetic resonance imaging, and correlate the imaging findings with the results of surgical pathology gross examination. (author)

  12. Prenatal diagnosis of conjoined twins by magnetic resonance imaging: report of two cases; Diagnostico pre-natal de gemeos unidos com uso da ressonancia nuclear magnetica: relato de dois casos

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Alex Sandro Rolland de; Medeiros, Cynthia Coelho de; Lins, Glaucia Virginia de Queiroz [Instituto Materno Infantil Professor Fernando Figueira (IMIP), Recife, PE (Brazil); Noronha Neto, Carlos [Centro Integrado de Saude Amaury de Medeiros (CISAM), Recife, PE (Brazil); Lima, Marcelo Marques de Souza [Universidade Federal de Sao Paulo (UNIFESP/EPM), Sao Paulo, SP (Brazil)]. E-mail: alexrolland@uol.com.br

    2006-07-15

    Conjoined twins have a rare prevalence and special curiosity among physicians and the general population. The reported frequency varies from 1:50,000 to 1:200,000 pregnancies. Its early diagnosis becomes very important when we think about pregnancy management, method of delivery and neonatal care. We describe two cases of conjoined twins diagnosed by ultrasound and magnetic resonance during prenatal care with the aim to better studying the fetus anatomy. The first conjoined twins were cephalopagus sharing head, thorax and abdominal wall and with two pelvis and four arms and four legs. The second were thoracopagus, united by thorax and part of abdomen. Magnetic resonance imaging contribution was not important to diagnose conjoined twins. However, it was useful to describe the shared organs, contributing to define fetal outcome. (author)

  13. Comparative study between body and surface coils in magnetic resonance mammography of silicone prosthesis; Estudo comparativo entre bobinas de corpo e superficie na mamografia por ressonancia magnetica de proteses de silicone

    Energy Technology Data Exchange (ETDEWEB)

    Scaranelo, Anabel Medeiros [Universidade Federal de Sao Paulo (UNIFESP), SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: anabelms@uol.com.br

    2001-04-01

    Magnetic resonance imaging scans using predefined parameters were performed in patients with silicone breast implants. The same group of patients was submitted to magnetic resonance imaging scans using surface breast coils and body coils, and the results were compared. A total of 43 single-lumen silicone-gel breast implants in 24 patients were examined. The signal-to-noise ratio was greater for the breast coil than for the body coil. Radial folds were identified with equal resolution by both in almost 82% of the cases on the right side and 95% on the left side. In about 5% of the cases the folds were seen exclusively when the breast coil used. The linguine sign was almost equally with both methods. In just one case the linguine sign was observed only by using the breast coil. Identification of building or irregularity of contours were concordant using both techniques. We concluded that although magnetic resonance imaging quality is better using a dedicated coil, silicone breast implants can be assessed with the same diagnostic accuracy using a body coil. (author)

  14. Magnetic resonance imaging in the evaluation of standard radiotherapy field borders in patients with uterine cervix cancer;Ressonancia magnetica para avaliacao dos limites dos campos classicos de radioterapia em pacientes portadoras de neoplasia maligna de colo uterino

    Energy Technology Data Exchange (ETDEWEB)

    Freire, Geison Moreira; Dias, Rodrigo Souza; Giordani, Adelmo Jose; Segreto, Helena Regina Comodo; Segreto, Roberto Araujo, E-mail: segreto.dmed@epm.b [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Unit of Radioterapy; Ribalta, Julisa Chamorro Lascasas [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Dept. de Gynecology

    2010-05-15

    Objective: to evaluate, by means of magnetic resonance imaging, the standardized field borders in radiotherapy for malignant neoplasm of uterine cervix, and to determine the role of this method in the reduction of possible planning errors related to the conventional technique. Materials and methods: magnetic resonance imaging studies for planning of treatment of 51 patients with uterine cervix cancer were retrospectively analyzed. The parameters assessed were the anterior and posterior field borders on sagittal section. Results: The anterior field border was inappropriate in 20 (39.2%) patients and geographic miss was observed in 37.3% of cases in the posterior border. The inappropriateness of both field borders did not correlate with clinical parameters such as patients' age, tumor staging, histological type and degree. Conclusion: the evaluation of standardized field borders with the use of magnetic resonance imaging has demonstrated high indices of inappropriateness of the lateral field borders, as well as the relevant role of magnetic resonance imaging in the radiotherapy planning for patients with uterine cervix cancer with a view to reduce the occurrence of geographic miss of the target volume. (author)

  15. Prominent porto-systemic collateral pathways in patients with portal hypertension: demonstration by gadolinium-enhanced magnetic resonance angiography; Vias colaterais porto-sistemicas exuberantes em portadores de hipertensao portal: demonstracao pela angiografia por ressonancia magnetica com gadolinio

    Energy Technology Data Exchange (ETDEWEB)

    Caldana, Rogerio Pedreschi; Bezerra, Alexandre Araujo Sergio; Cecin, Alexnadre Oliveira; Souza, Luis Ronan Marques Ferreira de; Goldman, Susan Menasce; D' Ippolito, Giuseppe; Szejnfeld, Jacob [Universidade Federal de Sao Paulo (UNIFESP/EPM), Sao Paulo, SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: rogercal@uol.com.br

    2003-03-01

    To demonstrate the usefulness of gadolinium-enhanced magnetic resonance angiography in the evaluation of prominent porto-systemic collateral pathways. We reviewed the images from 40 patients with portal hypertension studied with gadolinium-enhanced magnetic resonance angiography and selected illustrative cases of prominent porto-systemic collateral pathways. The scans were performed using high field equipment (1.5 Tesla) and a 3 D volume technique. Image were obtained after intravenous injection of paramagnetic contrast media using a power injector. Magnetic resonance angiography demonstrated with precision the porto-systemic collateral pathways, particularly when investigating extensive territories or large vessels. The cases presented show the potential of this method in the investigation of patients with portal hypertension. Gadolinium-enhanced magnetic resonance angiography is a useful method for the evaluation of patients with portal hypertension and prominent collateral pathways. (author)

  16. Proceedings of the 11. Brazilian meeting on magnetic resonance. Short courses on NMR. Abstracts and program; Anais da 11. Jornada brasileira de ressonancia magnetica. Mini-cursos em RMN. Resumos e programa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This annual meeting, held in Brazil from August 4 - 6, 2010 comprised : mini-courses about the use of nuclear magnetic resonance for various analytical purposes; major conferences and short conferences with invited speakers from Brazil and other countries; results from research works, most being carried out by scientific groups from various Brazilian R and D institutions, presented as oral communications and as congress panels/posters. Prizes to remarkable works of undergrad and post-grad students have been awarded, and a General Assembly meeting of AUREMN, the Brazilian Association of Nuclear Magnetic Resonance Users, also took place at the end of the event. Main topics of the research works presented at this meeting were thus distributed: 52% in chemical sciences (mainly organic chemistry, both experimental and theoretical works), 28% in applied life sciences (agricultural and food sciences, biological sciences and medicine), 18% in materials science (including nanocomposites, petroleum and alternative fuels), and 4% regarding development of equipment accessories for improving NMR techniques and environmental science-related themes

  17. Value of magnetic resonance imaging in the radiotherapy planning of tumours of the uterine cervix: preliminary results; Valor da ressonancia magnetica no planejamento radioterapico dos tumores de colo de utero: resultados preliminares

    Energy Technology Data Exchange (ETDEWEB)

    Justino, Pitagoras Baskara; Carvalho, Heloisa de Andrade [Sao Paulo Univ., SP (Brazil). Hospital das Clinicas. Inst. de Radiologia]. E-mail: pitagorasb@hotmail.com; Baroni, Ronaldo Hueb; Blasbalg, Roberto; Leite, Claudia da Costa [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Radiologia

    2005-12-01

    Objective: To assess the rate of geographic miss on conventional radiotherapy planning of patients with cervical cancer, using magnetic resonance imaging. Materials and methods: Thirty-two patients with squamous cell carcinoma of the uterine cervix were studied. Magnetic resonance imaging of the pelvis was performed after clinical staging. Magnetic resonance imaging findings were compared with the classic fields described for the 'box' technique. Target volume within less than 1 cm margins of the fields' limits was considered as geographic miss. Results: Classical radiation field limits were inadequate in 24 cases (75%), all in the anterior (46%) or posterior (40%) border of the lateral fields. Conclusion: Magnetic resonance detected a high probability of geographic miss on conventional radiotherapy planning in this population, both in initial and advanced stages of the disease. (author)

  18. Creutzfeldt-Jakob disease, Heidenhain variant: case report with MRI (DWI) findings; Doenca de Creutzfeldt-Jakob forma Heidenhain: relato de caso com achados de ressonancia magnetica e DWI

    Energy Technology Data Exchange (ETDEWEB)

    Arruda, Walter Oleschko; Bordignon, Kelly C; Milano, Jeronimo B; Ramina, Ricardo [Instituto de Neurologia de Curitiba, PR (Brazil)

    2004-06-01

    Creutzfeldt-Jakob disease (CJD) is a pre senile dementia characterized by rapidly progressive mental deterioration, myoclonic jerking, and other less common neurological signs. Few accentuates cases have been described in Brazil. A 54-year-old white woman, was admitted in our service with a month history of progressive, bilateral cortical blindness. After admission, she developed right partial motor seizures (right facial, upper and lower limbs), she became progressively aphasic (mixed aphasia). Seizures were controlled with phenytoine, but she developed choreoathetotic movements on her right dimidium, with partial control after introduction of chlorpromazine 25 mg q/d. She could no longer stand up or walk due to severe ataxia. The first EEG (October, 2001) showed left hemisphere severe seizure activity (status epilepticus partial is). She was delivered home with enteral nutrition, phenytoine, chlorpromazine and mepacrine 100 mg q d. The following laboratory tests were negative or normal: blood series, platelets, ESR, kidney and liver function, copper, ceruloplasmin, Vedril, HIV, HTLV-1, lactate, and cerebral Dsa (performed in other service). A spinal tap with normal opening pressure was perform and CSFR examination was normal. CSFR 14-3-3 protein was positive, CSF specific neuronal enolase 7.5 ng/ml(normal). Genetic study of PRNP gene did not disclosed any known mutation. A MRI (October, 2001) showed areas of hyperintense signal (T 2 and FLAIR) without Gd-enhancement on T1, in the left temporal lobe and in both occipital lobes; basal ganglia have a normal appearance. DWI imaging showed bright areas at the same sites. An EEG (March, 2002) disclosed a periodical sharp triphasic waves pattern, suggestive of CJD. A second MRI (April, 2002) showed mild generalized atrophy, no ventricular dilatation, and the hyperintense sites disappeared. She remained clinically stable and under use of chlorpromazine and mepacrine until she died due to pulmonary complications on April, 2003. (author)

  19. Gene Expression Profile in the Liver of BALB/c Mice Infected with Fasciola hepatica.

    Science.gov (United States)

    Rojas-Caraballo, Jose; López-Abán, Julio; Fernández-Soto, Pedro; Vicente, Belén; Collía, Francisco; Muro, Antonio

    2015-01-01

    Fasciola hepatica infection still remains one of the helminthic neglected tropical diseases (NTDs). It has a huge worldwide distribution, affecting mainly cattle and, sometimes, human beings. In addition to data reported about the immunological response induced by helminthic infections and that induced by Fasciola hepatica, little is known about the gene expression profile in its organ target, the liver, which is where adult worms are established and live for long periods of time, causing its characteristic pathology. In the present work, we study both the early and late gene expression profiles in the livers of mice infected with F. hepatica metacercariae using a microarray-based methodology. A total of 9 female-6-week-old BALB/c mice (Charles River Laboratories, Barcelona, Spain) weighing 20 to 35 g were used for the experiments. Two groups of BALB/c mice were orally infected with seven F. hepatica metacercariae, and the other group remained untreated and served as a control. Mice were humanely euthanized and necropsied for liver recovery, histological assessment of hepatic damage, RNA isolation, microarray design and gene expression analysis on the day of infection (t0), seven days post-infection (t7) and twenty-one days post-infection (t21). We found that F. hepatica infection induces the differential expression of 128 genes in the liver in the early stage of infection and 308 genes in the late stage, and most of them are up-regulated. The Ingenuity Pathway Analysis revealed significant changes in the pathways related to metabolism, biosynthesis and signaling as well as genes implicated in inducing liver-toxicity, injury and death. The present study provides us insights at the molecular level about the underlying mechanisms used by F. hepatica, leading to liver damage and its subsequent pathophysiology. The expression pattern obtained here could also be used to explain the lack of association between infection with F. hepatica and cholangiocarcinoma. However

  20. First insight into CD59-like molecules of adult Fasciola hepatica.

    Science.gov (United States)

    Shi, Yunliang; Toet, Hayley; Rathinasamy, Vignesh; Young, Neil D; Gasser, Robin B; Beddoe, Travis; Huang, Weiyi; Spithill, Terry W

    2014-09-01

    The present study focussed on investigating CD59-like molecules of Fasciola hepatica. A cDNA encoding a CD59-like protein (termed FhCD59-1) identified previously in the membrane fraction of the F. hepatica tegument was isolated. This homologue was shown to encode a predicted open reading frame (ORF) of 122 amino acids (aa) orthologous to human CD59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved CD59/Ly-6 family motif "CCXXXXCN". An analysis of cDNAs from two different adult specimens of F. hepatica revealed seven variable types of FhCD59-1 sequences, designated FhCD59-1.1 to FhCD59-1.7, which had 94.3-99.7% amino acid sequence identity upon pairwise comparison. Molecular modeling of FhCD59-1.1 with human CD59 confirmed the presence of the three-finger protein domain found in the CD59 family and predicted three disulphide bonds in the F. hepatica sequence. The interrogation of F. hepatica databases identified two additional sequences, designated FhCD59-2 and FhCD59-3, which had only 23.4-29.5% amino acid identity to FhCD59-1.1. Orthologues of the inferred CD59 protein sequences of F. hepatica were also identified in other flatworms, including Fasciola gigantica, Fascioloides magna, Schistosoma haematobium, Schistosoma japonicum, Schistosoma mansoni, Clonorchis sinensis, Opisthorchis viverrini, Taenia solium, Echinococcus granulosus and the free living Schmidtea mediterannea. The results revealed a considerable degree of sequence complexity in the CD59-like sequence families in F. hepatica and flatworms. Phylogenetic analysis of CD59-like aa sequences from F. hepatica and flatworms showed that FhCD59-2 clustered with the known surface-associated protein SmCD59-2 of S. mansoni. Relatively well-supported clades specific to schistosomes, fasciolids and opisthorchiids were identified. The qPCR analysis of gene transcription showed that the relative expression of these 3 FhCD59-like sequences varied by 11-47-fold during fluke

  1. Comparison of humoral response in sheep to Fasciola hepatica and Fasciola gigantica experimental infection

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    Zhang W.

    2004-06-01

    Full Text Available Humoral response of sheep to F. gigantica was compared with the well known humoral response to F. hepatica, in order to explain the difference of susceptibility of sheep to these two parasites. In this work, a lesser susceptibility of sheep to F. gigantica than to F. hepatica infection was confirmed. Humoral response to F. hepatica infection is similar to that previously described by several authors. IgG level of F. gigantica infected sheep increased from week 2 post-infection (2WPI and displayed a peak at 13WPI. F. gigantica excretory-secretory products (FgESP analyzed by SDS-PAGE showed at least 31 bands from 12.0 to 127.6 kDa in FgESP. Western blot indicated that F. gigantica infected sheep sera recognized, in FgESP, at least 30 antigens from 7.8 to 119.2 kDa of which 12 major bands recognized after OWPI. In FhESP and FgESP, F. hepatica infected sheep serum reacted only with the lower molecular mass antigens, while F. gigantica infected sheep serum reacted with the lower and the higher molecular mass antigens. These differences of antigenic recognition might be associated with the difference of susceptibility of sheep. Further investigation must be done to study the mechanism of resistance between the sheep infected with F. hepatica or F. gigantica.

  2. Dynamics of haemocytes from Pseudosuccinea columella circulating infected by Fasciola hepatica

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    Vinicius Marques Antunes Ribeiro

    2017-11-01

    Full Text Available Abstract The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been observed at 30 minutes post-infection and 1, 7, 10, 14, 21, 28 and 50 days post-infection (dpi. Miracidia were observed head-foot and mantle at 30 minutes post-infection. Miracidia/Sporocysts in the mantle skirt 1 dpi, and fully formed sporocysts were observed in the head-foot at 7 dpi. Rediae became evident at 10 dpi and were located between the haemocoel and the muscles from 14 dpi; 50 dpi, the rediae in the digestive gland contained cercariae. The statistical analysis of the total haemocytes of P. columella infected by F. hepatica showed significant differences on the 30 minutes post-infection and 1, 14, 21, and 28 dpi in comparison to uninfected molluscs (0 dpi. Therefore, the interference observed on the internal defence system of P. columella may have direct association with the development of F. hepatica.

  3. Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica.

    Science.gov (United States)

    Itagaki, T; Ichinomiya, M; Fukuda, K; Fusyuku, S; Carmona, C

    2011-09-01

    Experiments on hybridization between Fasciola hepatica and Fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two Fasciola species. Molecular evidence for hybridization was based on the DNA sequence of the internal transcribed spacer 1 (ITS1) region in nuclear ribosomal DNA, which differs between the species. The results suggested that there were not pre-mating but post-mating isolating mechanisms between the two species. However, viable adults of the hybrids F1 and F2 were produced from both parental F. hepatica and F. gigantica. The hybrids inherited phenotypic characteristics such as ratio of body length and width and infectivity to rats from parental Fasciola hepatica and F. gigantica. These findings suggest that reproductive isolation is incomplete between Fasciola hepatica and F. gigantica. Adults of the hybrids F1 and F2 were completely different in mode of reproduction from aspermic Fasciola forms that occur in Asia and seem to be offspring originated from hybridization between F. hepatica and F. gigantica and to reproduce parthenogenetically.

  4. Fasciola hepatica in Cuba: compatibility of different isolates with two intermediate snail hosts, Galba cubensis and Pseudosuccinea columella.

    Science.gov (United States)

    Vázquez, A A; Sánchez, J; Pointier, J-P; Théron, A; Hurtrez-Boussès, S

    2014-12-01

    In Cuba, only two lymnaeid snails, Galba cubensis and Pseudosuccinea columella, with different ecology and distribution patterns, are intermediate hosts for Fasciola hepatica. The compatibility of these two species as hosts was analysed through their rates of infection, the production of rediae and survivorship when exposed to F. hepatica miracidia. Ten populations of G. cubensis, eight of P. columella collected from various habitats and six isolates of F. hepatica sampled in slaughterhouses from different localities were tested. Our results clearly demonstrate that G. cubensis is a more compatible host for F. hepatica in Cuba when compared with P. columella. However, the role that P. columella may have in fascioliasis transmission under certain conditions should not be disregarded. Variation in infectivity among isolates of F. hepatica were also observed and may explain why some regions in Cuba are more commonly subjected to fascioliasis outbreaks.

  5. CT features of liver abscesses caused by the fasciola hepatica infection

    International Nuclear Information System (INIS)

    Fan Dong; Li Peng; Sun Hua; Wang Zhihua; She Bo

    2006-01-01

    Objective: To study CT features of liver abscesses caused by the fasciola hepatica infection, and discuss its pathologic basis. Methods: CT images of 15 Patients were collected. All patients underwent both unenhanced and biphasic enhanced CT scanning, then its CT performances were analyzed. Results: round and nodular lesions were observed in 15 cases, branching and stripping lesions like dilated bile duct in 9 cases. The density of lesions was inhomogeneous, and the lesions were multifocal and multiform. The liver abscesses caused by the fasciola hepatica infection had no 'rim sign' or 'target' sign, Liver abscesses were less than 3.0 cm in diameter, and the dilation of the bile duct were not observed. Conclusion: Liver abscessed caused by the fasciola hepatica infection have characteristic CT features. Combined with clinical examination and laboratory test, the reliability of diagnosis will be considerably increased. (authors)

  6. Fasciola hepatica saposin-like-2 protein based ELISA for the serodiagnosis of chronic human fascioliasis

    Science.gov (United States)

    Figueroa-Santiago, Olgary; Delgado, Bonnibel; Espino, Ana M.

    2011-01-01

    An indirect enzyme-linked immunosorbent assay (ELISA) was developed and evaluated for its diagnostic ability to detect human IgG antibodies against Fasciola hepatica saposin-like protein-2. The assay was compared with an indirect ELISA with excretory-secretory products (FhES) from adult F. hepatica. In an analysis of the sera of 37 patients infected with F. hepatica, 40 patients with other parasitic infections, and 50 healthy controls, the sensitivity of both ELISA assays was 100%. However, the FhSAP2-based ELISA was more specific (95.6%) than the FhES-ELISA (91.9%). These results demonstrated that FhSAP2 can be used in the serodiagnosis of chronic human fascioliasis with additional advantage that is relative cheap and easy to produce. Studies are in progress to evaluate this FhSAP2-ELISA assay in a large-scale prevalence surveys in endemic areas. PMID:21683266

  7. Heme-Oxygenase-1 Expression Contributes to the Immunoregulation Induced by Fasciola hepatica and Promotes Infection

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    Paula Carasi

    2017-07-01

    Full Text Available Fasciola hepatica, also known as the liver fluke, is a trematode that infects livestock and humans causing fasciolosis, a zoonotic disease of increasing importance due to its worldwide distribution and high economic losses. This parasite immunoregulates the host immune system by inducing a strong Th2 and regulatory T immune response by immunomodulating dendritic cell (DC maturation and alternative activation of macrophages. In this paper, we show that F. hepatica infection in mice induces the upregulation of heme-oxygenase-1 (HO-1, the rate-limiting enzyme in the catabolism of free heme that regulates the host inflammatory response. We show and characterize two different populations of antigen presenting cells that express HO-1 during infection in the peritoneum of infected animals. Cells that expressed high levels of HO-1 expressed intermediate levels of F4/80 but high expression of CD11c, CD38, TGFβ, and IL-10 suggesting that they correspond to regulatory DCs. On the other hand, cells expressing intermediate levels of HO-1 expressed high levels of F4/80, CD68, Ly6C, and FIZZ-1, indicating that they might correspond to alternatively activated macrophages. Furthermore, the pharmacological induction of HO-1 with the synthetic metalloporphyrin CoPP promoted F. hepatica infection increasing the clinical signs associated with the disease. In contrast, treatment with the HO-1 inhibitor SnPP protected mice from parasite infection, indicating that HO-1 plays an essential role during F. hepatica infection. Finally, HO-1 expression during F. hepatica infection was associated with TGFβ and IL-10 levels in liver and peritoneum, suggesting that HO-1 controls the expression of these immunoregulatory cytokines during infection favoring parasite survival in the host. These results contribute to the elucidation of the immunoregulatory mechanisms induced by F. hepatica in the host and provide alternative checkpoints to control fasciolosis.

  8. In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment

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    Russell M. Morphew

    2014-06-01

    Full Text Available The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ is threatened by emerging resistance. F. hepatica excretory/secretory (ES products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.

  9. Scintigraphic evaluation of the liver in Fasciola hepatica with radiocolloid and /sup 67/Ga-citrate

    Energy Technology Data Exchange (ETDEWEB)

    Aguirre Errasti, C.; Alberola Gomez-Escolar, I.; Gonzalez de Zarate, P.; Merino Angulo, J.

    1981-02-01

    A group of 23 patients with Fasciola hepatica have been studied in the province of Biscay. The diagnosis was reached after finding parasite eggs in the faeces or duodenal juice. The liver lesion was confirmed by means of macro and microscopic studies. Radiocolloid demonstrated the presence of cold areas in 18 patients; in 13 of them the uptake with /sup 67/Ga was positive. These isotopic findings identified Fasciola hepatica as one of the causes of 'cold areas' in traditional liver scans and positive /sup 67/Ga uptake.

  10. Scintigraphic evaluation of the liver in Fasciola hepatica with radiocolloid and 67Ga-citrate

    International Nuclear Information System (INIS)

    Aguirre Errasti, C.; Alberola Gomez-Escolar, I.; Gonzalez de Zarate, P.; Merino Angulo, J.

    1981-01-01

    A group of 23 patients with Fasciola hepatica have been studied in the province of Biscay. The diagnosis was reached after finding parasite eggs in the faeces or duodenal juice. The liver lesion was confirmed by means of macro and microscopic studies. Radiocolloid demonstrated the presence of cold areas in 18 patients; in 13 of them the uptake with 67 Ga was positive. These isotopic findings identified Fasciola hepatica as one of the causes of 'cold areas' in traditional liver scans and positive 67 Ga uptake. (orig.)

  11. Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line; Marcacao intracelular e processo de quantificacao por imagem por ressonancia magnetica utilizando nanoparticulas magneticas de oxido de ferro em celulas da linhagem C6 de glioma de rato

    Energy Technology Data Exchange (ETDEWEB)

    Mamani, Javier Bustamante; Pavon, Lorena Favaro; Sibov, Tatiana Tais; Rossan, Fabiana; Silveira, Paulo Henrique; Cardenas, Walter Humberto; Gamarra, Lionel Fernel, E-mail: javierbm@einstein.br [Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Miyaki, Liza Aya Mabuchi [Faculdade de Enfermagem, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Amaro Junior, Edson [Departamento de Diagnostico por Imagem e Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil)

    2012-04-15

    Objective: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications. Methods: Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification process was performed by relaxometry analysis in T{sub 1} and T{sub 2} weighted phantom images. Results: Light microscopy revealed that the aminosilane-coated magnetic nanoparticles alone or complexed with poly-L-lysine showed higher cellular uptake than did the uncoated magnetic particles. The relaxactivities of the aminosilane-coated magnetic nanoparticles with a hydrodynamic diameter of 50nm to a 3-T field were r{sub 1}=(6.1 +- 0.3) x10{sup -5} ms{sup -1}mL/{mu}g, r{sub 2}=(5.3 +- 0.1) x 10{sup -4} ms{sup -1}mL/{mu}g, with a ratio of r{sub 2} / r{sub 1}{approx_equal} 9. The iron uptake in the cells was calculated by analyzing the relaxation rates (R{sub 1}and R{sub 2}) using a mathematical relationship. Conclusions: C6 glioma cells have a high uptake efficiency for aminosilane-coated magnetic nanoparticles complexed with the transfection agent poly-L-lysine. The large ratio r{sub 2} / r{sub 1}{approx_equal} 9 indicates that these magnetic nanoparticles are ideal for quantification by magnetic resonance imaging with T{sub 2}-weighted imaging techniques. (author)

  12. Predicting impacts of climate change on Fasciola hepatica risk.

    Science.gov (United States)

    Fox, Naomi J; White, Piran C L; McClean, Colin J; Marion, Glenn; Evans, Andy; Hutchings, Michael R

    2011-01-10

    Fasciola hepatica (liver fluke) is a physically and economically devastating parasitic trematode whose rise in recent years has been attributed to climate change. Climate has an impact on the free-living stages of the parasite and its intermediate host Lymnaea truncatula, with the interactions between rainfall and temperature having the greatest influence on transmission efficacy. There have been a number of short term climate driven forecasts developed to predict the following season's infection risk, with the Ollerenshaw index being the most widely used. Through the synthesis of a modified Ollerenshaw index with the UKCP09 fine scale climate projection data we have developed long term seasonal risk forecasts up to 2070 at a 25 km square resolution. Additionally UKCIP gridded datasets at 5 km square resolution from 1970-2006 were used to highlight the climate-driven increase to date. The maps show unprecedented levels of future fasciolosis risk in parts of the UK, with risk of serious epidemics in Wales by 2050. The seasonal risk maps demonstrate the possible change in the timing of disease outbreaks due to increased risk from overwintering larvae. Despite an overall long term increase in all regions of the UK, spatio-temporal variation in risk levels is expected. Infection risk will reduce in some areas and fluctuate greatly in others with a predicted decrease in summer infection for parts of the UK due to restricted water availability. This forecast is the first approximation of the potential impacts of climate change on fasciolosis risk in the UK. It can be used as a basis for indicating where active disease surveillance should be targeted and where the development of improved mitigation or adaptation measures is likely to bring the greatest benefits.

  13. Predicting impacts of climate change on Fasciola hepatica risk.

    Directory of Open Access Journals (Sweden)

    Naomi J Fox

    2011-01-01

    Full Text Available Fasciola hepatica (liver fluke is a physically and economically devastating parasitic trematode whose rise in recent years has been attributed to climate change. Climate has an impact on the free-living stages of the parasite and its intermediate host Lymnaea truncatula, with the interactions between rainfall and temperature having the greatest influence on transmission efficacy. There have been a number of short term climate driven forecasts developed to predict the following season's infection risk, with the Ollerenshaw index being the most widely used. Through the synthesis of a modified Ollerenshaw index with the UKCP09 fine scale climate projection data we have developed long term seasonal risk forecasts up to 2070 at a 25 km square resolution. Additionally UKCIP gridded datasets at 5 km square resolution from 1970-2006 were used to highlight the climate-driven increase to date. The maps show unprecedented levels of future fasciolosis risk in parts of the UK, with risk of serious epidemics in Wales by 2050. The seasonal risk maps demonstrate the possible change in the timing of disease outbreaks due to increased risk from overwintering larvae. Despite an overall long term increase in all regions of the UK, spatio-temporal variation in risk levels is expected. Infection risk will reduce in some areas and fluctuate greatly in others with a predicted decrease in summer infection for parts of the UK due to restricted water availability. This forecast is the first approximation of the potential impacts of climate change on fasciolosis risk in the UK. It can be used as a basis for indicating where active disease surveillance should be targeted and where the development of improved mitigation or adaptation measures is likely to bring the greatest benefits.

  14. First report of Lymnaea columella Say, 1817 (Pulmonata: Lymnaeidae naturally infected with Fasciola hepatica (Linnaeus,1758 (Trematoda: Digenea in Argentina

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    Prepelitchi Lucila

    2003-01-01

    Full Text Available We report the first evidence of natural infection of Lymnaea columella with Fasciola hepatica in Argentina. A sample of 601 snails was collected in May 2003 in northeastern Corrientes, a province bounded on the north by Paraguay, on the east by Brazil and on the southeast by Uruguay. Among 500 examined snails, 44 (8.8% were exclusively infected with F. hepatica. Parasite identification was based on morphological features of cercariae from snails, and of eggs and adult flukes from Wistar rats. We discuss the events suggesting that an enzootic transmission cycle of F. hepatica has been recently established in northeastern Corrientes.

  15. The energy metabolism of Fasciola hepatica during its development in the final host

    NARCIS (Netherlands)

    Tielens, A.G.M.; Heuvel, J.M. van den; Bergh, S.G. van den

    1984-01-01

    Mature liver flukes, Fasciola hepatica, of different ages were isolated from the bile ducts of experimentally infected rats. Their energy metabolism was studied during aerobic incubation with [6-14C]glucose. The results showed that the aerobic potentials of the parenchymal liver flukes are not lost

  16. Differences in intermediary energy metabolism between juvenile and adult Fasciola hepatica

    NARCIS (Netherlands)

    Tielens, A.G.M.; Heuvel, J.M. van den; Bergh, S.G. van den

    A comparison of glucose catabolism by juvenile and adult liver flukes, Fasciola hepatica, showed that in the adult the cytosolic degradation of glucose via phosphoenolpyruvate carboxykinase (PEPCK) was the most important route, whereas in the freshly excysted juvenile a large part was degraded via

  17. 31P NMR studies of pH homeostasis in intact adult Fasciola hepatica

    NARCIS (Netherlands)

    Tielens, A.G.M.; Nicolaij, K.; Bergh, van S.G.

    1982-01-01

    31P NMR was used to measure the intracellular pH in live adult Fasciola hepatica. The results demonstrate that at external pH values above 7.0, pH homeostasis keeps the intracellular pH at 7.0. At external pH values below 7.0 the intracellular pH is less strictly regulated.

  18. Aneurysm of the A. hepatica propria as cause of Angina abdominalis

    International Nuclear Information System (INIS)

    Rieser, R.; Hauger, W.

    1988-01-01

    The aetiology of a. hepatica aneurysm can be manifold. Among all the complications, rupture of the vessel is the most dangerous one. We report on a case which was noticed clinically due to an angina abdominalis. In this paper we outline the symptoms, all diagnostic steps and the therapeutic approach. (orig.) [de

  19. Phenotypes of intermediate forms of Fasciola hepatica and F. gigantica in buffaloes from Central Punjab, Pakistan.

    Science.gov (United States)

    Afshan, K; Valero, M A; Qayyum, M; Peixoto, R V; Magraner, A; Mas-Coma, S

    2014-12-01

    Fascioliasis is an important food-borne parasitic disease caused by the two trematode species, Fasciola hepatica and Fasciola gigantica. The phenotypic features of fasciolid adults and eggs infecting buffaloes inhabiting the Central Punjab area, Pakistan, have been studied to characterize fasciolid populations involved. Morphometric analyses were made with a computer image analysis system (CIAS) applied on the basis of standardized measurements. Since it is the first study of this kind undertaken in Pakistan, the results are compared to pure fasciolid populations: (a) F. hepatica from the European Mediterranean area; and (b) F. gigantica from Burkina Faso; i.e. geographical areas where both species do not co-exist. Only parasites obtained from bovines were used. The multivariate analysis showed that the characteristics, including egg morphometrics, of fasciolids from Central Punjab, Pakistan, are between F. hepatica and F. gigantica standard populations. Similarly, the morphometric measurements of fasciolid eggs from Central Punjab are also between F. hepatica and F. gigantica standard populations. These results demonstrate the existence of fasciolid intermediate forms in endemic areas in Pakistan.

  20. Changes in energy metabolism of the juvenile Fasciola hepatica during its development in the liver parenchyma

    NARCIS (Netherlands)

    Tielens, A.G.M.; Heuvel, J.M. van den; Bergh, S.G. van den

    1982-01-01

    Juvenile Fasciola hepatica at different stages of development were isolated from the liver parenchyma of experimentally infected rats. Their energy metabolism was studied by incubation with D-[16-14C]glucose and compared with that of juveniles isolated immediately after in vitro emergence from the

  1. Fasciola hepatica: comparative metacercarial productions in experimentally-infected Galba truncatula and Pseudosuccinea columella

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    Vignoles Philippe

    2015-01-01

    Full Text Available As large numbers of metacercariae of Fasciola hepatica are necessary for research, experimental infections of Galba truncatula and Pseudosuccinea columella with this digenean were carried out to determine the better intermediate host for metacercarial production and, consequently, the most profitable snail for decreasing the cost price of these larvae. Pre-adult snails (4 mm in shell height originating from two populations per lymnaeid species were individually exposed to two or five miracidia, raised at 23 °C and followed for cercarial shedding up to their death. Compared to values noted in G. truncatula, the survival of P. columella on day 30 post-exposure was significantly greater, while the prevalence of F. hepatica infection was significantly lower. In the four P. columella groups, metacercarial production was significantly greater than that noted in the four groups of G. truncatula (347–453 per cercariae-shedding snail versus 163–275, respectively. Apart from one population of G. truncatula, the use of five miracidia per snail at exposure significantly increased the prevalence of F. hepatica in P. columella and the other population of G. truncatula, whereas it did not have any clear effect on the mean number of metacercariae. The use of P. columella for experimental infections with F. hepatica resulted in significantly higher metacercarial production than that noted with G. truncatula, in spite of a lower prevalence for the former lymnaeid. This finding allows for a significant decrease in the cost price of these larvae for commercial production.

  2. Fibrinogen and fibrin are novel substrates for Fasciola hepatica cathepsin L peptidases

    NARCIS (Netherlands)

    Mebius, Mirjam M.; Op Heij, Jody M J; Tielens, Aloysius G.M.; de Groot, Philip G; Urbanus, Rolf T; van Hellemond, Jaap J.

    2018-01-01

    Cathepsin peptidases form a major component of the secreted proteins of the blood-feeding trematodes Fasciola hepatica and Schistosoma mansoni. These peptidases fulfill many functions, from facilitating infection to feeding and immune evasion. In this study, we examined the Fasciola cathepsin L

  3. Composition and metabolism of phospholipids of Fasciola hepatica, the common liver fluk

    NARCIS (Netherlands)

    Oldenborg, V.; Vugt, F. van; Golde, L.M.G. van

    1. 1. The phospholipid composition of Fasciola hepatica, the common liver fluke, was compared to that of the liver of the host animals (rats and cattle). Considerable differences were found: monoacyl-sn-glycero-3-phosphorylcholine, hardly detectable in the liver, was found in significant amounts in

  4. Studies on Colombian Cryptogams VI. High Andean species of Radula (Hepaticae)

    NARCIS (Netherlands)

    Jans, Els

    1979-01-01

    This study deals with the genus Radula (Hepaticae) from the high Andean forests and paramos of Colombia, above 2500 m, and is based on the determination of Colombian collections gathered by H. Bischler and by A. M. Cleef and collaborators. A key to the 8 species known from the area is given and

  5. Protection against heterologous infection by using cross antigenicity between schistosoma mansoni and fasciola hepatica

    International Nuclear Information System (INIS)

    Moawad, M.A.; Amin, M.M.

    2004-01-01

    Fasciola hepatica is the causative agent of fasciolosis in many areas in America, Europe, Africa, Asia and Australia. There is an urgent need for improved methods to control the parasite's transmission. The present study is parasitological, immunological (interleukine-1β and interleukine-6) and histopathological investigations on the immunizing effect of cross antigenicity between S. mansoni and F. hepatica against schistosomiasis and fasciolosis in mice. Parasitological study showed that vaccination with irradiated cercariae of S. mansoni or vaccination by F. hepatica whole worm extract (FhWWE) before challenged with encysted metacercariae of F. hepatica or cercariae of S. mansoni played a significant control on the parasitic infection manifested by a remarkable reduction in the means of worm count. Assessment of IL-1β and IL-6 in sera of the experimental groups showed that there are cross reactivity between fasciola / schistosoma and its relation to cross protection. Histopathological examination of vaccinated mice livers showed protection against parasite maturation and liver damage after challenged, as compared to mice infected only without vaccination

  6. Molecular differentiation of sheep and cattle isolates of Fasciola hepatica using RAPD-PCR

    Directory of Open Access Journals (Sweden)

    Khakpour, M.

    2012-11-01

    Full Text Available Understanding genetic structure and status of genetic variation of Fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. Random amplified polymorphic DNA (RAPD-PCR was used to study the genetic variation of F. hepatica in sheep and cattle. DNA was extracted from adult helminthes removed from livers of each infected animal in slaughterhouse at East-Azerbaijan province, North-West of Iran. DNA template amplified by the polymerase chain reaction, using three oligonucleotide decamers with arbitrary DNA sequences as primers. RAPD patterns showed the specific but different pattern DNA patterns for each primer. The intraspecific similarity coefficient within two isolates of F. hepatica was ranged between 69 to 100%. Present findings showed that the interspecific genetic distance was higher than intraspecific genetic distances (19-47% compares to 0-19%. Pair wise similarity matrices generated from each isolates-primer combination were totaled and the similarity coefficient between strains were calculated both manually (Nei and Li method and software analysis (Free-Tree-Freeware program. The inferred phylogenetic tree on the fingerprinting of these isolates clearly demonstrated the existence of population genetic diversity sub structuring within F. hepatica of sheep and cattle of Iran, raising interesting questions on the host specificity, epidemiology (e.g., zoonotic transmission and ecology of this fluke. RAPD-PCR is useful for both individual identification and epidemiological investigations in endemic regions.

  7. Gene Expression Profile in the Liver of BALB/c Mice Infected with Fasciola hepatica.

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    Jose Rojas-Caraballo

    Full Text Available Fasciola hepatica infection still remains one of the helminthic neglected tropical diseases (NTDs. It has a huge worldwide distribution, affecting mainly cattle and, sometimes, human beings. In addition to data reported about the immunological response induced by helminthic infections and that induced by Fasciola hepatica, little is known about the gene expression profile in its organ target, the liver, which is where adult worms are established and live for long periods of time, causing its characteristic pathology. In the present work, we study both the early and late gene expression profiles in the livers of mice infected with F. hepatica metacercariae using a microarray-based methodology.A total of 9 female-6-week-old BALB/c mice (Charles River Laboratories, Barcelona, Spain weighing 20 to 35 g were used for the experiments. Two groups of BALB/c mice were orally infected with seven F. hepatica metacercariae, and the other group remained untreated and served as a control. Mice were humanely euthanized and necropsied for liver recovery, histological assessment of hepatic damage, RNA isolation, microarray design and gene expression analysis on the day of infection (t0, seven days post-infection (t7 and twenty-one days post-infection (t21.We found that F. hepatica infection induces the differential expression of 128 genes in the liver in the early stage of infection and 308 genes in the late stage, and most of them are up-regulated. The Ingenuity Pathway Analysis revealed significant changes in the pathways related to metabolism, biosynthesis and signaling as well as genes implicated in inducing liver-toxicity, injury and death.The present study provides us insights at the molecular level about the underlying mechanisms used by F. hepatica, leading to liver damage and its subsequent pathophysiology. The expression pattern obtained here could also be used to explain the lack of association between infection with F. hepatica and cholangiocarcinoma

  8. Distribution of Fasciola hepatica in Swedish dairy cattle and associations with pasture management factors

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    Adam Novobilský

    2015-03-01

    Full Text Available The geographic distribution of Fasciola hepatica infection in relation to management routines was studied in Swedish dairy herds by testing for F. hepatica antibodies with the enzyme-linked immunosorbent assay (ELISA. In addition, all farmers were sent a questionnaire asking for information about type of production, management routines and historical record of F. hepatica at slaughter. A total of 176 farmers (41% responded to the questionnaire. A total of 426 bulk tank milk (BTM samples were randomly selected from the period September to October 2012 representing approximately 10% of all herds in Sweden. The overall seroprevalence was 25% (n = 107; 95% confidence interval = 21-29% with a concentration of herds located in south-western Sweden. Among the seropositive herds, 31 (29% had antibody levels indicating production loss. There were no significant differences in seropositivity between organic and conventional herds or due to pasture management routines. The length of grazing period, which increased the risk for heifers, was found to be the most influential factor. A discrepancy was noted between reported F. hepatica presence at meat inspection and herds that were seropositive based on BTM-ELISA results. Although the largest proportion of seropositive BTM samples (80% came from herds where liver fluke presence had been observed at meat inspection after slaughter, seropositive BTM samples were also diagnosed in five herds (17% with no remarks at slaughter. In conclusion, F. hepatica is a common parasite in Swedish dairy herds and the month of heifer turn-out and the grazing period length were the most influential factors observed.

  9. Secreted products of Fasciola hepatica inhibit the induction of T cell responses that mediate allergy.

    Science.gov (United States)

    Finlay, C M; Stefanska, A M; Coleman, M M; Jahns, H; Cassidy, J P; McLoughlin, R M; Mills, K H G

    2017-10-01

    There is evidence from epidemiology studies of a negative association between infection with helminth parasites and the development of allergy and asthma. Here, we demonstrate that the excretory/secretory products of the helminth Fasciola hepatica (FHES) protected mice against ovalbumin (OVA)-induced allergic asthma when administered at time of allergen sensitization. FHES reduced the accumulation of mucus, eosinophils and lymphocytes into the airways of allergen-challenged mice. Furthermore, FHES treatment suppressed Th2 responses in the airways. Interestingly, systemic administration of FHES at allergen challenge had no effect on airway inflammation, demonstrating that alum-induced Th2 response is set following initial allergen sensitization. Our findings highlight the immunomodulatory potential of molecules secreted by F. hepatica. © 2017 John Wiley & Sons Ltd.

  10. Nöropati ile başvuran Fasciola hepatica olgusu

    OpenAIRE

    BOĞA, Salih; KÖKSAL, Ali Rıza; ALTINKAYA, Engin; ÖZDOĞAN, Osman; ERGÜN, Meltem; ALKIM, Canan ALATAŞ

    2015-01-01

    Fascioliazis insanda fasciola türlerinin, özellikle Fasciola hepatica'nın neden olduğu zoonotik bir hastalıktır. Fascioliazis koyun, sığır gibi ot yiyen evcil hayvanlarda yaygın olarak görülmekle beraber insanlar rastlantı sal konaklardır. ‹nsan enfeksiyonu genellikle koyun yetiştirilen yerlerde su teresi tüketilmesiyle alır. Erişkin Fasciola hepatica 2.5–3 cm boyunda 1–1.5 cm çapında yaprak şekilli bir yassı solucandır. Fasciola kistleri asıl konak veya insanlar tarafından ora...

  11. Partial purification and properties of adenosine triphosphatase (ATPase) from liver fluke Fasciola hepatica.

    Science.gov (United States)

    Hassan, Husain; Abeer, Ali

    2014-01-01

    The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.;ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source. The adenosine triphosphatase (ATPase ; EC: 3.6.1.3) was extracted from membrane preparations of adult Fasciola hepatica by chloroform treatment and purified by means of ammonium sulphate fractionation, gel filtration on sephadex G-200 and DEAE- Cellulose chromatography. The molecular weight was calculated to be 305.000 dalton by gel filtration. Kinetic experiments demonstrated a biphasic linear lineweaver - burk relationship (km=0.142 and 1.66 mM) thus revealing the existence of two substrate binding enzyme sites. In our study revealed that partial inhibition of Mg²⁺ dependent purified enzyme by oligomycin suggest the absence of mitochondrial ATPase in F. hepatica.

  12. In vitro antihelmintic effect of fifteen tropical plant extracts on excysted flukes of Fasciola hepatica

    OpenAIRE

    Alvarez-Mercado, Jos? Manuel; Ibarra-Velarde, Froyl?n; Alonso-D?az, Miguel ?ngel; Vera-Montenegro, Yolanda; Avila-Acevedo, Jos? Guillermo; Garc?a-Bores, Ana Mar?a

    2015-01-01

    Background Fasciolosis due to Fasciola hepatica is the most important hepatic disease in veterinary medicine. Its relevance is important because of the major economical losses to the cattle industry such as: reduction in milk, meat and wool production; miscarriages, anemia, liver condemnation and occasionally deaths, are estimated in billons of dollars. The emergence of fluke resistance due to over or under dosing of fasciolides as well as environmental damage produced by the chemicals elimin...

  13. Triclabendazole Effect on Protease Enzyme Activity in the Excretory- Secretory Products of Fasciola hepatica in Vitro.

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    Yosef Shrifi

    2014-03-01

    Full Text Available Fasciola hepatica is one of the most important helminthes parasites and triclabendazole (TCBZ is routinely used for treatment of infected people and animals. Secreted protease enzymes by the F. hepatica plays a critical role in the invasion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. The aim of study was to determine the protease activity of excretory- secretory products (ESP of F. hepatica in the presence of TCBZ anthelmintic.F. hepatica helminthes were collected and cultured within RPMI 1640 [TCBZ treated (test and untreated (control] for 6 h at 37 °C. ESP of treated and control were collected, centrifuged and supernatants were stored at -20°C. Protein concentrations were measured according to Bradford method. Protease enzymes activities of ESP samples were estimated by using sigma's non-specific protease activity assay. ESP protein bands were detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE.Mean protein concentrations in control and treated of ESP samples were determined 196.1 ±14.52 and 376.4 ±28.20 μg/ml, respectively. Mean protease enzymes activities in control and treated were 0.37 ±0.1 and 0.089 ±0.03 U/ml, respectively. Significant difference between proteins concentrations and protease enzymes activities of two groups was observed (P<0.05. SDS-PAGE showed different patterns of protein bands between treated and control samples.The TCBZ reduced secreted protease enzymes activities and possibly effects on invasion, migration, nutrition and particularly survival of the parasite in the host tissues.

  14. Burden of Fasciola hepatica Infection among Children from Paucartambo in Cusco, Peru

    OpenAIRE

    Lopez, Martha; White, A. Clinton; Cabada, Miguel M.

    2012-01-01

    There is a high prevalence of fascioliasis in the Peruvian highlands, but most cases remain undiagnosed. The burden of disease caused by chronic subclinical infection is largely unknown. We studied school-age children from a district in Paucartambo Province in Cusco, Peru to evaluate the burden of disease caused by subclinical fascioliasis. Parasite eggs and/or larvae were identified in 46.2% of subjects, including Fasciola hepatica in 10.3% of subjects. Fascioliasis was independently associa...

  15. Clinical presentation and management of Fasciola hepatica infection: Single-center experience

    Science.gov (United States)

    Kaya, Muhsin; Beştaş, Remzi; Çetin, Sedat

    2011-01-01

    AIM: To identify the characteristic clinical, laboratory and radiological findings and response to treatment in patients with fascioliasis. METHODS: Patients who were diagnosed with Fasciola hepatica infection were included in this prospective study. Initial clinical, laboratory and radiological findings were recorded. All patients were followed until a complete response was achieved or for 6 mo after treatment discontinuation. RESULTS: Fasciola hepatica infection was diagnosed in 30 patients (24 females; mean age: 42.6 years) between January 2008 and February 2011. Twenty-two (73%) patients had hepatic phase fascioliasis, 5 patients had biliary phase, and 3 patients had biliary phase associated with acute pancreatitis. Of the 8 patients with biliary phase fascioliasis, 2 patients displayed features that overlapped with both hepatic and biliary phase. Abdominal pain and right upper abdominal tenderness were the most prominent signs and symptoms in all patients. Eosinophilia was the most prominent laboratory abnormality in both patients with hepatic and biliary phase (100% and 50%, respectively). Multiple nodular lesions like micro-abscesses on abdominal computerized tomography were the main radiological findings in patients with hepatic phase. Small linear filling defects in the distal choledochus were the main endoscopic retrograde cholangiopancreatography (ERCP) findings in patients with biliary phase. Patients with hepatic phase were treated with triclabendazole alone, and patients with biliary phase were treated with triclabendazole and had live Fasciola hepatica extracted from the bile ducts during ERCP. CONCLUSION: Fasciola hepatica infection should be considered in the differential diagnosis of patients with hepatic or biliary disease and/or acute pancreatitis associated with eosinophilia. PMID:22171131

  16. Fasciola hepatica infection and association with gastrointestinal parasites in Creole goats from western Argentina.

    Science.gov (United States)

    Cuervo, Pablo; Sidoti, Laura; Fantozzi, Cecilia; Neira, Gisela; Gerbeno, Leticia; Mera y Sierra, Roberto

    2013-01-01

    Goats, called “the cow of the poor”, are the livestock species with the most significant population growth worldwide in recent years. Gastrointestinal parasitism constitutes one of the main constraints to its outdoor and extensive breeding in temperate and tropical countries. Despite a Creole goat population of nearly 4 million heads, local reports on parasitological prevalence are scarce, and while Fasciola hepatica infection is spread all over Argentina, the goat is usually neglected as a reservoir and economic losses are not considered. To evaluate gastrointestinal parasitism prevalence and associations between parasite genera and species, with emphasis on fascioliasis, Creole goats from the plateau and Andean regions from western Argentina were investigated by coprological techniques, and associations were statistically assessed. Eighty-five percent (85%) of the animals harbored one or more parasite types, while 46% showed mixed infections. Significant positive associations between F. hepatica + Strongyle eggs, Eimeria sp. + Nematodirus sp. and Nematodirus sp. + Trichuris ovis were detected. Further studies are required to define the causality of these associations and their relevance in epidemiology. F. hepatica is rarely considered as goat parasite in the country, but a 33% prevalence poses an interrogation on the role goats play on the transmission and dissemination of this zoonotic trematode.

  17. Localization of serotonin and ultrastructure of serotonergic neutrons in the nervous system of fasciola hepatica

    International Nuclear Information System (INIS)

    Huang Shile; Cheng Bing; Rong Yaofang

    1993-01-01

    Rabbits antisera were raised against an antigen prepared by coupling 5-HT to bovine serum albumin (BSA) using formaldehyde as a coupling reagent. The fresh adult Fasciola hepatica were fixed with 4% formaldehyde and sectioned on a cryostat. The sections were stained by indirect immunofluorescence technique. Abundant immunofluorescence specific for 5-HT was observed in ganglion cell bodies and their processes, the transverse commissure that connects two ganglia and longitudinal axes extending from the ganglia. Immuno-reactivity to 5-HT was also found in the nerve fibre innervating tegument, gut wall, the epithelium of testes or ovary, the musculature of uterus and ootype, etc. The ultrastructure of serotonergic neurons was visualized. As in other invertebrates, the serotonergic neutrons of Fasciola hepatica consisted of cell bodies, axons, synapses, herring bodies and neuromuscular junctions. The nerve cell bodies were aggregatively located in ganglia and many dispersed spherical granular vesicles were present in cytoplasm. The nerve axons branched out to the muscles forming synapses, where synaptic vesicles contained 5-HT dense-core granules were found. The distribution of 5-HT within the neurons strongly suggested that 5-HT was functioning as a neurotrasmitter in Fasciola hepatica

  18. Development of indirect sandwich ELISA for determination of excretory-secretory antigens of Fasciola hepatica

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    Libertad Alzamora-Gonzales

    2016-05-01

    Full Text Available Fasciolosis is a cosmopolitan parasitosis medical-veterinary importance caused by Fasciola hepatica, which affects sheep, goats and cattle; and it affects man accidentally causing an epidemic-endemic infection difficult to diagnose. The aim was to develop an indirect sandwich ELISA with 3 antibodies for detecting excretory-secretory antigens of Fasciola hepatica (ESFh. For the development of indirect sandwich ELISA were used, as capture antibody, mouse polyclonal antibodies anti ESFh and polyclonal antibodies rabbit anti-ESFh as detection antibody, at the concentrations of 10 and 5 µg/mL respectively. The conjugate used was mouse monoclonal anti- total immunoglobulins rabbit linked to peroxidase (1/1000. Were analized 31 sheep fecal samples, and the results were compared with those obtained by direct coproparasitological examination (DC and counterimmunoelectrophoresis (CIEP. The detection limit obtained for indirect sandwich ELISA was 100 ng/mL. The test had a 100% sensitivity, 96.6% specificity, positive and negative predictive values of 50% and 96.6% respectively, in relation to DC test. Comparing with CIEP the specificity obtained for indirect sandwich ELISA was 93.5% and a negative predictive value of 100%. We concluded that indirect sandwich ELISA designed is able to detect metabolic antigens in ovine feces samples and can be used for Fasciola hepatica diagnosis.

  19. CIAS detection of Fasciola hepatica/F. gigantica intermediate forms in bovines from Bangladesh.

    Science.gov (United States)

    Ahasan, Syed Ali; Valero, M Adela; Chowdhury, Emdadul Haque; Islam, Mohammad Taohidul; Islam, Mohammad Rafiqul; Hussain Mondal, Mohammad Motahar; Peixoto, Raquel V; Berinde, Lavinia; Panova, Miroslava; Mas-Coma, Santiago

    2016-03-01

    Fascioliasis is an important food-borne parasitic zoonosis caused by two trematode species, Fasciola hepatica and Fasciola gigantica. The characterisation and differentiation of Fasciola populations is crucial to control the disease, given the different transmission, epidemiology and pathology characteristics of the two species. Lineal biometric features of adult liver flukes infecting livestock have been studied to characterise and discriminate fasciolids from Bangladesh. An accurate analysis was conducted to phenotypically discriminate between fasciolids from naturally infected bovines (cattle, buffaloes) throughout the country. Morphometric analyses were made with a computer image analysis system (CIAS) applied on the basis of standardised measurements and the logistic model of the body growth and development of fasciolids in the different host groups. Since it is the first ever comprehensive study of this kind undertaken in Bangladesh, the results are compared to pure fasciolid populations of F. hepatica from the European Mediterranean area and F. gigantica from Burkina Faso, geographical areas where both species do not co-exist. Principal component analysis showed that the biometric characteristics of fasciolids from Bangladesh are situated between F. hepatica and F. gigantica standard populations, indicating the presence of phenotypes of intermediate forms in Bangladesh. These results are analysed by considering the present emergence of animal fascioliasis, the local lymnaeid fauna, the impact of climate change, and the risk of human infection in the country.

  20. Influence of Fasciola Hepatica on Serum Biochemical Parameters and Vascular and Biliary System of Sheep Liver

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    A Hodžić

    2013-03-01

    Full Text Available Background: The aim of this study was to evaluate the functional capacity of the liver based on the activity of specific enzymes and bilirubin in serum and also to investigate the influence of mechanical and toxic effects of Fasciola hepatica on the structures of the blood vessels and biliary tract in the sheep liver.Methods: Blood samples and liver of 63 indigenous sheep of Pramenka breed, slaughtered in the period from March to December 2009 were used. Based on parasitological findings in the liver, all animals were divided into two groups: control (n=34 and infected group (n=29. For investigation and description of pathological changes in sheep liver, naturally infected with F. hepatica, corrosion cast technique was used.Results: Biochemical analysis of tested parameters showed a significant elevation (P≤0.05 of serum gamma-glutamyl transferase (GGT, total bilirubin (TBIL and direct bilirubin (DBIL in infected sheep group comparing with the control group. No significant differences were observed for activity of aspartate aminotranferase (AST between groups. Vascular and biliary systems of the liver were found to be affected.Conclusion: Results of biochemical analysis are consistent with pathological findings and measuring of tested parameters could be used in early diagnosis of sheep fasciolosis and to test the effectiveness of anthelmintic therapy. Corrosion cast technique is very useful for investigation of pathological changes and neoangiogenesis of vascular and biliary system in sheep liver, caused by mechanical and toxic effects of F. hepatica.

  1. Human fascioliasis by Fasciola hepatica: the first case report in Nepal.

    Science.gov (United States)

    Sah, Ranjit; Khadka, Shusila; Khadka, Mohan; Gurubacharya, Dipesh; Sherchand, Jeevan Bahadur; Parajuli, Keshab; Shah, Niranjan Prasad; Kattel, Hari Prasad; Pokharel, Bharat Mani; Rijal, Basista

    2017-09-05

    Fascioliasis is a zoonotic disease caused by Fasciola species. Patient may be asymptomatic or presents with jaundice and biliary colic or right hypochondriac pain due to bile duct obstruction with gastrointestinal symptoms. We report a case of human fascioliasis in a 45 years old female presented to Tribhuvan University Teaching Hospital (TUTH), Kathmandu, Nepal on August, 2015 with fever, right hypochondriac pain, jaundice and occasional vomiting with anorexia for 4 months whose alkaline phosphatase was elevated and peripheral blood smear revealed eosinophilia. The patient also gives the history of consumption of water-cress. Endoscopic Retrograde Cholagiopancretography (ERCP) showed the presence of a flat worm resembling Fasciola hepatica and stool routine examination revealed ova of F. hepatica. The patient was treated with nitazoxanide by which she got improved. Repeat stool examination 2 weeks after treatment revealed no ova of F. hepatica. Patient with fascioliasis can be simply diagnosed with stool routine microscopy and treated with nitazoxanide. So patient with right hypochondriac pain, sign and symptoms of obstructive jaundice, eosinophilia and history of water-cress consumption should be suspected for fascioliasis and investigated and treated accordingly.

  2. Fasciola hepatica infection reduces Mycobacterium bovis burden and mycobacterial uptake and suppresses the pro-inflammatory response.

    Science.gov (United States)

    Garza-Cuartero, L; O'Sullivan, J; Blanco, A; McNair, J; Welsh, M; Flynn, R J; Williams, D; Diggle, P; Cassidy, J; Mulcahy, G

    2016-07-01

    Bovine tuberculosis (BTB), caused by Mycobacterium bovis, has an annual incidence in cattle of 0.5% in the Republic of Ireland and 4.7% in the UK, despite long-standing eradication programmes being in place. Failure to achieve complete eradication is multifactorial, but the limitations of diagnostic tests are significant complicating factors. Previously, we have demonstrated that Fasciola hepatica infection, highly prevalent in these areas, induced reduced sensitivity of the standard diagnostic tests for BTB in animals co-infected with F. hepatica and M. bovis. This was accompanied by a reduced M. bovis-specific Th1 immune response. We hypothesized that these changes in co-infected animals would be accompanied by enhanced growth of M. bovis. However, we show here that mycobacterial burden in cattle is reduced in animals co-infected with F. hepatica. Furthermore, we demonstrate a lower mycobacterial recovery and uptake in blood monocyte-derived macrophages (MDM) from F. hepatica-infected cattle which is associated with suppression of pro-inflammatory cytokines and a switch to alternative activation of macrophages. However, the cell surface expression of TLR2 and CD14 in MDM from F. hepatica-infected cattle is increased. These findings reflecting the bystander effect of helminth-induced downregulation of pro-inflammatory responses provide insights to understand host-pathogen interactions in co-infection. © 2016 The Authors. Parasite Immunology Published by John Wiley & Sons Ltd.

  3. Aspects of the Maintenance of the Life Cycle of Fasciola hepatica in Lymnaea columella in Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Souza Cecília Pereira de

    2002-01-01

    Full Text Available Fascioliasis is a parasitic disease of domestic ruminants that occurs worldwide. The lymnaeid intermediate hosts of Fasciola hepatica include Lymnaea columella, which is widely distributed in Brazil. A colony of L. columella from Belo Horizonte, MG, was reared in our laboratory to be used in studies of the F. hepatica life cycle, the intermediate host-parasite relationship and development of an anti-helminthic vaccine. In the first experiment 1,180 snails were exposed to miracidia of F. hepatica eggs removed from the biliary tracts of cattle from the State of Rio Grande do Sul. In the second and third experiments the snails were exposed to miracidia that had emerged from F. hepatica eggs from Uruguay, maintained in rabbits. The rates of infection in the first, second and third experiments were 0, 42.1 and 0% respectively. Over 15,806 metacercariae were obtained and stored at 4ºC. Four rabbits weighing 1.5 kg each were infected with 32-44 metacercariae and two with 200. Three rabbits begin to eliminate eggs of the parasite in the feces from 84 days after infection onwards. The biological cycle of F. hepatica in L. columella and the rabbit was completed within 124 days.

  4. MRI and MRCP of chronic pancreatitis. 1: technique and methods; La valutazione delle pancreatiti croniche con Risonanza Magnetica e colangiopancreatografia con Risonanza Magnetica. Prima parte: aspetti tecnico-metodologici

    Energy Technology Data Exchange (ETDEWEB)

    Laghi, A.; Pavone, P.; Catalano, C.; Panebianco, V.; Luccichenti, G.; Fabiano, S.; Passariello, R. [Rome Univ. La Sapienza, Rome (Italy). Ist. di Radiologia

    1999-10-01

    Until recently, MR examinations of the pancreas were limited by motion artifacts, vascular pulsatility and poor spatial resolution. Today, new techniques have been developed, which allow to overcome these problems and provide additional information such as selective images of biliary and pancreatic ducts and vascular structures. As a complement to baseline sequences, MR cholangiopancreatography images can be acquired, possibly integrated by functional examination after secretin administration. Finally, contrast-enhanced MR angiography opens new perspectives for vascular studies, particularly for the locoregional staging of pancreatic cancer. [Italian] Lo studio del pancreas con risonanza magnetica e' stato fino a pochi anni fa limitato dagli artefatti da movimento, da pulsatilita' vascolare e dalla ridotta risoluzione spaziale. Recentemente sono state sviluppate nuove tecniche che consentono da un lato di risolvere tali problemi e dall'altro di fornire informazioni aggiuntive, quali immagini selettive delle vie biliari e del dotto pancreatico e delle strutture vascolari. Come completamento all'esame di base si possono ottenere immagini di colangiopancreatografia con RM, eventualmente integrate da prove funzionali, rappresentate dall'iniezione di secretina. Infine, le nuove tecniche di angiografia con RM con mdc, nelle quali si effettua un'acquisizione durante la prima fase di passaggio arterioso o venoso dopo introduzione rapida del mdc a livello di una vena periferica, aprono nuove prospettive per lo studio vascolare, in particolare nella stadiazione loco-regionale dei processi neoplastici.

  5. Sympatric and allopatric combinations of Lymnaea columella and Fasciola hepatica from southern and south-eastern Brazil.

    Science.gov (United States)

    Coelho, L H L; Lima, W S; Guimaraes, M P

    2009-09-01

    Experimental infections of Lymnaea columella with Fasciola hepatica were carried out to test the effect of sympatric and allopatric combinations between parasite and host, by using snails and flukes from southern and south-eastern Brazil. Four groups of 50 snails were infected with four miracidia per snail: two groups in sympatric and two groups in allopatric combinations. Sympatric combinations between parasite and host were more efficient than allopatric ones when snails from Itajubá were used, but the opposite was observed in infections involving snails from Pelotas. The sympatric association between L. columella and F. hepatica from Itajubá was significantly higher than in the other combinations. We concluded that the host-parasite relationship between L. columnella and F. hepatica may vary according to the geographical origin of the snails and flukes involved.

  6. Elucidating the transcriptome of Fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite.

    Science.gov (United States)

    Young, Neil D; Hall, Ross S; Jex, Aaron R; Cantacessi, Cinzia; Gasser, Robin B

    2010-01-01

    Liver flukes of animals are parasitic flatworms (Platyhelminthes: Digenea) of major socioeconomic importance in many countries. Key representatives, such as Fasciola hepatica and F. gigantica, cause "liver fluke disease" (= fascioliasis), which is of major animal health significance worldwide. In particular, F. hepatica is a leading cause of production losses to the livestock (mainly sheep and cattle) and meat industries due to clinical disease, reduced weight gain and milk production, and deaths. This parasite is also a major food-borne pathogen of humans throughout parts of the Middle East, Asia and South America. Currently, there is a significant focus on the development of new approaches for the prevention and control of fascioliasis in livestock. Recent technological advances in genomics and bioinformatics provide unique opportunities for the identification and prevalidation of drug targets and vaccines through a better understanding of the biology of F. hepatica and related species as well as their relationship with their hosts at the molecular level. Surprisingly, despite the widespread socioeconomic impact of fascioliasis, genomic datasets for F. hepatica are scant, limiting the molecular biological research of this parasite. The present article explores specifically the transcriptome of the adult stage of F. hepatica using an integrated genomic-bioinformatic platform. The analysis of the current data reveals numerous molecules of biological relevance, some of which are inferred to be involved in key biological processes or pathways that could serve as targets for new trematocidal drugs or vaccines. Improved insights into the transcriptome of F. hepatica should pave the way for future, comparative analysis of the transcriptomes of other developmental stages of this and related parasites, such as F. gigantica, cancer-causing flatworms (Clonorchis sinensis and Opisthorchis viverrini) and blood flukes (Schistosoma mansoni and S. japonicum). Prediction of the

  7. Understanding the main route of drug entry in adult Fasciola hepatica: Further insights into closantel pharmacological activity.

    Science.gov (United States)

    Ceballos, L; Canton, C; Cadenazzi, G; Larsen, K; Virkel, G; Moreno, L; Fairweather, I; Lanusse, C; Alvarez, L

    2017-10-01

    Closantel (CLS) is highly effective against adult liver flukes after its oral or subcutaneous (sc) administration in ruminants. Trans-tegumental diffusion and oral ingestion are the two potential routes available for the entry of drugs into Fasciola hepatica. The work reported here contributes to improve the understanding of CLS pharmacology. The main goals of were: I) to determine the pattern of in vivo CLS accumulation into adult F. hepatica and relevant tissues in CLS-treated sheep; II) to investigate the influence of the physicochemical composition of the incubation medium on the CLS diffusion process into adult F. hepatica; III) to assess the ovicidal activity of CLS against F. hepatica eggs; and IV) to investigate the in vivo effect of CLS treatment on glutathione S-transferases activity in adult liver flukes exposed to CLS. Fourteen healthy sheep were each orally infected with 75 F. hepatica metacercariae. Sixteen (16) weeks after infection, animals were treated with CLS by oral (n = 6, 10 mg/kg) or sub-cutaneous (sc) (n = 6, 5 mg/kg) route. At 12, 24 and 36 h post-treatment, animals were sacrificed (n = 2) and samples of blood, bile and adult F. hepatica were collected. In addition, flukes recovered from non-treated sheep (n = 2) were ex vivo incubated (60 min) in the presence of CLS in either RPMI or bile as incubation medium. CLS concentration was measured by HPLC. The ovicidal activity of CLS was investigated using eggs obtained from the bile of untreated sheep. Finally, glutathione S-transferase activity in F. hepatica recovered from untreated and CLS-treated sheep was assessed. In the in vivo studies, the highest CLS concentrations were measured in plasma and adult liver flukes. A positive correlation was observed between CLS concentration in plasma and in F. hepatica. Results obtained in the current work indicate that the in vivo accumulation of CLS into adult liver flukes occurs mainly by the oral route. After ex

  8. Is Galba schirazensis (Mollusca, Gastropoda) an intermediate host of Fasciola hepatica (Trematoda, Digenea) in Ecuador?

    Science.gov (United States)

    Caron, Yannick; Celi-Erazo, Maritza; Hurtrez-Boussès, Sylvie; Lounnas, Mannon; Pointier, Jean-Pierre; Saegerman, Claude; Losson, Bertrand; Benítez-Ortíz, Washington

    2017-01-01

    Fasciolosis is a widely distributed disease in livestock in South America but knowledge about the epidemiology and the intermediate hosts is relatively scarce in Ecuador. For three months, lymnaeid snails were sampled (n = 1482) in Pichincha Province at two sites located in a highly endemic area. Snails were identified (based on morphology and ITS-2 sequences) and the infection status was established through microscopic dissection and a multiplex polymerase chain reaction (PCR)-based technique. Techniques based on morphology were not useful to accurately name the collected snail species. Comparison with available DNA sequences showed that a single snail species was collected, Galba schirazensis. Live rediae were observed in 1.75% (26/1482) and Fasciola sp. DNA was detected in 6% (89/1482) of collected snails. The COX-1 region permitted identification of the parasite as Fasciola hepatica. The relative sensitivity and specificity of the microscope study, compared to PCR results, were 25.84% and 99.78%, respectively. The mean size of the snails recorded positive for F. hepatica through crushing and microscopy was significantly higher than the mean size of negative snails, but there was no such difference in PCR-positive snails. The role of G. schirazensis as an intermediate host of F. hepatica in Ecuador is discussed and the hypothesis of an adaptation of the parasite to this invasive snail is proposed. For the first time, an epidemiological survey based on molecular biology-based techniques assessed the possible role of lymnaeid snails in the epidemiology of fasciolosis in Ecuador. © Y. Caron et al., published by EDP Sciences, 2017.

  9. Is Galba schirazensis (Mollusca, Gastropoda an intermediate host of Fasciola hepatica (Trematoda, Digenea in Ecuador?

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    Caron Yannick

    2017-01-01

    Full Text Available Fasciolosis is a widely distributed disease in livestock in South America but knowledge about the epidemiology and the intermediate hosts is relatively scarce in Ecuador. For three months, lymnaeid snails were sampled (n = 1482 in Pichincha Province at two sites located in a highly endemic area. Snails were identified (based on morphology and ITS-2 sequences and the infection status was established through microscopic dissection and a multiplex polymerase chain reaction (PCR-based technique. Techniques based on morphology were not useful to accurately name the collected snail species. Comparison with available DNA sequences showed that a single snail species was collected, Galba schirazensis. Live rediae were observed in 1.75% (26/1482 and Fasciola sp. DNA was detected in 6% (89/1482 of collected snails. The COX-1 region permitted identification of the parasite as Fasciola hepatica. The relative sensitivity and specificity of the microscope study, compared to PCR results, were 25.84% and 99.78%, respectively. The mean size of the snails recorded positive for F. hepatica through crushing and microscopy was significantly higher than the mean size of negative snails, but there was no such difference in PCR-positive snails. The role of G. schirazensis as an intermediate host of F. hepatica in Ecuador is discussed and the hypothesis of an adaptation of the parasite to this invasive snail is proposed. For the first time, an epidemiological survey based on molecular biology-based techniques assessed the possible role of lymnaeid snails in the epidemiology of fasciolosis in Ecuador.

  10. Seasonal pattern of Fasciola hepatica antibodies in dairy herds in Northern Germany.

    Science.gov (United States)

    Kuerpick, Birte; Schnieder, Thomas; Strube, Christina

    2012-09-01

    Fasciolosis, caused by the liver fluke Fasciola hepatica, is one of the most important parasitoses in cattle farming worldwide. In dairy cows, the trematode leads to economic losses due to decreased milk yield, a negative impact on reproduction parameters, and liver condemnations. In the present study, the seasonal patterns of F. hepatica antibodies in bulk-tank milk from dairy herds located in East Frisia, a region of the federal state Lower Saxony in the north of Germany, were investigated. This region was chosen since it is known as a high risk area for fluke infections due to its coastal location at the North Sea with the consequence of rather moist pastures. Between 669 and 868 bulk-tank milk samples were collected in January, September and November 2008 and 2010, respectively, and analysed for antibodies against F. hepatica with an in-house ELISA based on excretory-secretory antigens of the liver fluke. The overall East Frisian prevalence was 49.1%, 57.1% and 53.9% in January, September and November 2008 and 45.1%, 49.5% and 48.4% in 2010. From a number of 606 farms, which were sampled in all six investigated months, 34.5% of the farms continued to remain positive, whereas 30.9% continued to remain negative. A percentage of 69.1% (419 farms) were positive on at least one sampling occasion during the study period. The distributions of optical density ratio (ODR) values were skewed to the left but showed a second, lower peak in a high ODR range. Statistical analysis revealed a significant difference concerning the prevalence increase from January to September 2008. Furthermore, the prevalence decrease from September as well as November 2008 to these months in 2010 was significantly different, what might result from a more frequent use of anthelminthics or different climatic conditions.

  11. Genome Sizes in Hepatica Mill: (Ranunculaceae Show a Loss of DNA, Not a Gain, in Polyploids

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    B. J. M. Zonneveld

    2010-01-01

    , and a possible pentaploid. The somatic nuclear DNA contents (2C-value, as measured by flow cytometry with propidium iodide, were shown to range from 33 to 80 pg. The Asiatic and American species, often considered subspecies of H. nobilis, could be clearly distinguished from European H. nobilis. DNA content confirmed the close relationships in the Asiatic species, and these are here considered as subspecies of H. asiatica. Parents for the allotetraploid species could be suggested based on their nuclear DNA content. Contrary to the increase in genome size suggested earlier for Hepatica, a significant (6%–14% loss of nuclear DNA in the natural allopolyploids was found.

  12. Relative importance of meteorological and geographical factors in the distribution of Fasciola hepatica infestation in farmed sheep in Qinghai province, China

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    Qin Hongyu

    2016-01-01

    Full Text Available Fasciola hepatica is an important trematode parasite of economic importance that infests sheep and cattle worldwide. We conducted a detailed investigation into the spatial distribution of F. hepatica infestation in farmed sheep in Qinghai (Wutumeiren province, Mainland China. Mathematical modelling was used to assess the inter-relationships between meteorological and geographical factors and the risk of F. hepatica infestation across the province. A capture enzyme-linked immunosorbent assay (ELISA test (MM3-SERO was used to detect F. hepatica infestation. A niche model based on the maximum entropy method (MaxEnt was used to estimate the influence of meteorological and geographical factors on the observed spatial distribution of F. hepatica infestation. Results of jackknife analysis indicated that temperature, precipitation, solar radiation, digital elevation and slope were associated with the occurrence of F. hepatica infestation, and that infestation rates were significantly higher among animals from districts with a high percentage of grassland habitat. The findings indicate that meteorological and geographical factors may be important variables affecting the distribution of F. hepatica infestation and should be taken into account in the development of future surveillance and control programmes for fascioliasis.

  13. Fasciola hepatica IN BOVINES IN BRAZIL: DATA AVAILABILITY AND SPATIAL DISTRIBUTION

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    Sita C. Bennema

    2014-01-01

    Full Text Available Fasciolosis is a disease of importance for both veterinary and public health. For the first time, georeferenced prevalence data of Fasciola hepatica in bovines were collected and mapped for the Brazilian territory and data availability was discussed. Bovine fasciolosis in Brazil is monitored on a Federal, State and Municipal level, and to improve monitoring it is essential to combine the data collected on these three levels into one dataset. Data were collected for 1032 municipalities where livers were condemned by the Federal Inspection Service (MAPA/SIF because of the presence of F. hepatica. The information was distributed over 11 states: Espírito Santo, Goiás, Minas Gerais, Mato Grosso do Sul, Mato Grosso, Pará, Paraná, Rio de Janeiro, Rio Grande do Sul, Santa Catarina and São Paulo. The highest prevalence of fasciolosis was observed in the southern states, with disease clusters along the coast of Paraná and Santa Catarina and in Rio Grande do Sul. Also, temporal variation of the prevalence was observed. The observed prevalence and the kriged prevalence maps presented in this paper can assist both animal and human health workers in estimating the risk of infection in their state or municipality.

  14. On the presence and immunoregulatory functions of extracellular microRNAs in the trematode Fasciola hepatica.

    Science.gov (United States)

    Fromm, B; Ovchinnikov, V; Høye, E; Bernal, D; Hackenberg, M; Marcilla, A

    2017-02-01

    Liver flukes represent a paraphyletic group of endoparasitic flatworms that significantly affect man either indirectly due to economic damage on livestock or directly as pathogens. A range of studies have focussed on how these macroscopic organisms can evade the immune system and live inside a hostile environment such as the mammalian liver and bile ducts. Recently, microRNAs, a class of short noncoding gene regulators, have been proposed as likely candidates to play roles in this scenario. MicroRNAs (miRNAs) are key players in development and pathogenicity and are highly conserved between metazoans: identical miRNAs can be found in flatworms and mammalians. Interestingly, miRNAs are enriched in extracellular vesicles (EVs) which are secreted by most cells. EVs constitute an important mode of parasite/host interaction, and recent data illustrate that miRNAs play a vital part. We have demonstrated the presence of miRNAs in the EVs of the trematode species Dicrocoelium dendriticum and Fasciola hepatica (Fhe) and identified potential immune-regulatory miRNAs with targets in the host. After our initial identification of miRNAs expressed by F. hepatica, an assembled genome and additional miRNA data became available. This has enabled us to update the known complement of miRNAs in EVs and speculate on potential immune-regulatory functions that we review here. © 2016 John Wiley & Sons Ltd.

  15. In vivo assessment of closantel ovicidal activity in Fasciola hepatica eggs.

    Science.gov (United States)

    Solana, María Victoria; Mera y Sierra, Roberto; Scarcella, Silvana; Neira, Gisela; Solana, Hugo Daniel

    2016-01-01

    Anthelmintic resistance in livestock parasites is currently a worldwide problem. Fasciola hepatica is a cosmopolitan parasite which causes considerable loss in sheep and cattle production systems all over the world. Chemotherapy is currently the main tool available for its control. The intensive use of triclabendazole, the drug of choice for more than 20 years, has resulted in the development of resistant strains. The therapeutic options are adulticides such as closantel (salicylanilide anthelmintic that binds extensively to plasma albumin) to treat chronic fascioliasis in sheep, and cattle. In the present work, an Egg Hatch Assay (EHA) and morphometric studies were used to evaluate in vivo the ovicidal activity and morphology F. hepatica eggs, recovered from closantel treated sheep collected at different time intervals post treatment. Statistically significant differences (p < 0.0001) were observed in egg morphometry between the control and the treated groups in all the parameters studied. Eggs recovered from treated animals tend to be narrower and longer. Significant differences were found in the embryonation and hatching of eggs between 36 h post treatment (32, 5%) vs. approximately 85% in control, 12 h and 24 h post treatment. Our results confirm that closantel affects in vivo the normal development of the eggs. As one of the first effects, this drug affects the performance of the trematode's reproductive physiology. Even though closantel treated animals may still eliminate eggs in the first days post treatment, these are not viable. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Local adaptation of the trematode Fasciola hepatica to the snail Galba truncatula

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    Dreyfuss G.

    2012-08-01

    Full Text Available Experimental infections of six riverbank populations of Galba truncatula with Fasciola hepatica were carried out to determine if the poor susceptibility of these populations to this digenean might be due to the scarcity or the absence of natural encounters between these snails and the parasite. The first three populations originated from banks frequented by cattle in the past (riverbank group whereas the three others were living on islet banks without any known contact with local ruminants (islet group. After their exposure, all snails were placed in their natural habitats from the end of October up to their collection at the beginning of April. Compared to the riverbank group, snails, which died without cercarial shedding clearly predominated in the islet group, while the other infected snails were few in number. Most of these last snails released their cercariae during a single shedding wave. In islet snails dissected after their death, the redial and cercarial burdens were significantly lower than those noted in riverbank G. truncatula. Snails living on these islet banks are thus able to sustain larval development of F. hepatica. The modifications noted in the characteristics of snail infection suggest the existence of an incomplete adaptation between these G. truncatula and the parasite, probably due to the absence of natural contact between host and parasite.

  17. Comparative strategies and success of sympatric and allopatric Fasciola hepatica infecting Galba truncatula of different susceptibilities.

    Science.gov (United States)

    Sanabria, R; Mouzet, R; Courtioux, B; Vignoles, P; Rondelaud, D; Dreyfuss, G; Cabaret, J; Romero, J

    2013-06-01

    Allopatric infections of French Galba truncatula with an Argentinean isolate of Fasciola hepatica were carried out to determine the infectivity of foreign miracidia in three snail populations differing by their susceptibility to French miracidia (two highly and one poorly susceptible populations). Sympatric infections of G. truncatula with French miracidia were used as controls. Compared to sympatric infections of G. truncatula, snail survival at day 30 post-infection in allopatric groups was significantly lower in a highly susceptible population and significantly greater in the other two. Prevalence in snails infected with the allopatric isolate was significantly lower (16.4-34.5 % instead of 58.6-72.1 %), whereas their patent period was significantly longer (a mean of 69.9-85.9 days instead of 6.4-20.7 days). The mean number of metacercariae was also higher in allopatric groups (236.5-897.3 per cercariae-shedding snail instead of 70.7-222.1). Owing to longer patent periods, the Argentinean isolate of F. hepatica was less pathogenic for these snails. The lower prevalence of infection, the longer patent period and the higher number of metacercariae noted in allopatric groups might be the consequence of an adaptive mechanism used by this digenean introduced to the New World to infect new populations of unusual intermediate hosts.

  18. Evaluation and Characterization of Fasciola hepatica Tegument Protein Extract for Serodiagnosis of Human Fascioliasis

    Science.gov (United States)

    Morales, Adelaida

    2012-01-01

    Tegument protein extract from Fasciola hepatica adult flukes (FhTA) was obtained and assessed for its potential as a diagnostic agent for the serological detection of human fascioliasis using an indirect enzyme-linked immunosorbent assay (ELISA). In an analysis of sera from 45 patients infected with F. hepatica, sera from 41 patients with other parasitic infections, and sera from 33 healthy controls, the FhTA-ELISA showed sensitivity, specificity, and accuracy of 91.1%, 97.3%, and 95%, respectively. Specific IgG1 and IgG4 were the antibody isotypes mainly detected in sera from patients with fascioliasis. Polypeptides of 52, 38, 24 to 26, and 12 to 14 kDa were identified by Western blotting as the most immunoreactive components of the FhTA. A proteomic approach led us to identify enolase, aldolase, glutathione S-transferase, and fatty acid binding protein as the major immunoreactive components of the FhTA. PMID:23015645

  19. Carbohydrate Detection and Lectin Isolation from Tegumental Tissue of Fasciola hepatica

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    MB Molaei Rad

    2010-02-01

    Full Text Available "nBackground: Fascioliasis is a chronic hepatic disease and may be resulted from mechani­cal/molecular parasite adhesion to host liver tissue. The aim of this study was to detect surface car­bohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding."nMethods: The present experimental work was conducted in the Department of Medical Parasitol­ogy and Mycology, School of Public Health, Tehran University of Medical Sciences, Te­hran, Iran.  Fasciola hepatica parasites were collected from abattoir (Saman, Tehran, Iran and surface mannose-carbohydrate was detected by fluorescein isothiocyanate (FITC conju­gated lectin (Lentil. Lectin of tegumental tissue from F. hepatica was isolated by affinity chroma­tography and detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE."nResults: Mannose carbohydrate was observed on the surface of tegumental tissue from para­site under fluorescence microscope. Carbohydrate-binding protein or lectin with MW of 50 kDa also was isolated from homogenized tegument of helminth."nConclusion: These results are important for understanding of molecular pathogenesis of F. hepat­ica at the chronic phase of fascioliasis

  20. The presence of Fasciola hepatica (Liver-fluke in humans and cattle from a 4,500 Year old archaeological site in the Saale-Unstrut Valley, Germany

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    Dittmar K

    2003-01-01

    Full Text Available During an excavation of a site of the corded ware culture in the Saale-Unstrut-Valley (ca. 3000 BC in Germany, a soil sample from the pelvis of a human skeleton was studied under palaeoparasitological aspects. Eggs of the trematode Fasciola hepatica and of the nematode genus Capillaria were found. This is the first case of a direct association of a F. hepatica-infestation to both a prehistoric human skeleton and domesticated animal remains. Sheep and cattle bones were present at the same site and F. hepatica eggs were found in bovine samples. This strongly points toward an existing infection cycle, involving humans as a final host.

  1. Notes on the Plagiochilaceae, IX. A Review of the Genus Plagiochila (Dum.) Dum. (Hepaticae) in the Galapagos Islands

    NARCIS (Netherlands)

    Inoue, Hiroshi; Gradstein, S. Rob

    1980-01-01

    El género Plagiochila (hepatica) esta representada en las Islas Galapagos por ocho (8) especies diferentes: P. bursata (Desv.) Lindenbg., P. galapagona Inoue, P gradsteinii Inoue, P. guilleminiana Mont., P. inouei Grolle, P. scabrifolia Inoue, P. spinifera Ångstr. y P. subplana Lindenbg. El

  2. Consequence of temperature changes on cercarial shedding from Galba truncatula infected with Fasciola hepatica or Paramphistomum daubneyi

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    Rondelaud Daniel

    2013-01-01

    Full Text Available Experimental infections of Galba truncatula (two populations with Fasciola hepatica or Paramphistomum daubneyi were carried out to study the effect of water temperature changes (3 h at a mean of 12 °C every week on cercarial shedding during the patent period. The results were compared with those of control snails infected according to the same protocol and always maintained at 20 °C. Compared to controls, a significant increase in the number of cercariae-shedding snails, a significantly longer patent period and significantly greater cercarial production were noted in temperature-challenged snails, regardless of the type of digenean infection. In contrast, the number of incompletely formed metacercariae was significantly higher in temperature-challenged snails than in controls. Incompletely formed metacercariae of F. hepatica consisted of cysts whose colour remained whitish after shedding (25.4% for temperature-challenged snails or whose dome was flattened after encystment (74.6%. Those of P. daubneyi were totally dark brown or blackish after formation. These incomplete metacercariae might originate from young differentiating cercariae within the snail body (F. hepatica or from cercariae which died just after encystment (P. daubneyi. The use of regular temperature changes for snails infected with F. hepatica or P. daubneyi must be monitored carefully during collection of metacercariae to select completely formed cysts for infecting definitive hosts.

  3. Role of the lymnaeid snail Pseudosuccinea columella in the transmission of the liver fluke Fasciola hepatica in Egypt.

    Science.gov (United States)

    Dar, Y; Vignoles, P; Rondelaud, D; Dreyfuss, G

    2015-11-01

    Experimental infections of three Egyptian Pseudosuccinea columella populations with sympatric miracidia of Fasciola sp., coming from cattle- or sheep-collected eggs, were carried out to determine the capacity of this lymnaeid to support larval development of the parasite. Using microsatellite markers, the isolates of Egyptian miracidia were identified as Fasciola hepatica. Apart from being independent of snail origin, prevalences ranging from 60.4 to 75.5% in snails infected with five miracidia of F. hepatica were significantly higher than values of 30.4 to 42.2% in snails with bi-miracidial infections. The number of metacercariae ranged from 243 to 472 per cercarial-shedding snail and was independent of snail origin, parasite origin and miracidial dose used for infection. If P. columella was subjected to two successive bi-miracidial infections with F. hepatica, prevalence of infection was 63.3%, with a mean of 311 metacercariae per snail. These values were clearly greater than those already reported for Radix natalensis infected with the same parasite and the same protocol. Successful experimental infection of P. columella with F. hepatica suggests that this lymnaeid snail is an important intermediate host for the transmission of fascioliasis in Egypt.

  4. Variations in local adaptation of allopatric Fasciola hepatica to French Galba truncatula in relation to parasite origin.

    Science.gov (United States)

    Dar, Y; Lounnas, M; Djuikwo Teukeng, F F; Mouzet, R; Courtioux, B; Hurtrez-Boussès, S; Vignoles, P; Dreyfuss, G; Rondelaud, D

    2013-07-01

    Two French populations of Galba truncatula were subjected to experimental infections with Egyptian and French isolates of Fasciola sp. miracidia, originating from cattle and sheep, to compare characteristics of snail infections in allopatric and sympatric groups. All sampled Egyptian isolates were identified as Fasciola hepatica using microsatellite markers. Compared to snails infected with French miracidia, snail survival at day 30 post-exposure was significantly greater in the Egyptian groups, while prevalence of infection was significantly lower (in an Egyptian group infected with cattle-derived miracidia) or did not show any significant differences in the other three cases. The total number of metacercariae was significantly higher in the four Egyptian groups. However, snail population and the mammalian origin of F. hepatica had also a significant effect on this parameter. The dissection of snail cadavers showed a significantly higher number of free rediae in the Egyptian groups, even if snail population also had a significant effect on the redial burden. Both Egyptian isolates of F. hepatica could easily develop in French snails, causing a low mortality in snails and inducing a metacercarial production higher than that noted in sympatric infections. However, the mammalian origin of F. hepatica eggs and the quality of snail populations as intermediate hosts had to be taken into account for studying local adaptation in reason of their effects on this process.

  5. In Vitro Assay of Paecilomyces lilacinus Biocontrol Effects on Fasciola hepatica Eggs Illustrated in Scanning Electron Micrographs

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    Faezeh NAJAFI

    2017-02-01

    Full Text Available Background: Fascioliasis is a zoonotic disease caused by the liver fluke Fasciola hepatica. Drug resistance, high costs of treatment and economic losses in meat production have emerged the need of alternative control measures into consideration. The aim of this study was to evaluate the in vitro ovicidal activity of Paecilomyces lilacinus fungus on F. hepatica eggs. Methods: P. lilacinus isolated from the soil of natural environment was challenged on F. hepatica eggs to observe the bio control effect of nematophagous fungi on trematode helminth eggs. The study was conducted in Tehran University of Medical Sciences, in 2015. Within 21 d of experiment, destructive effects exhibited on the eggshells were investigated using optical and Scanning Electron Microscopy. Results: The effective role of P. lilacinus on damaging the eggs of F. hepatica was noticed. Conclusion: This finding is promising for advantageous use of nematophagus fungi as a natural constituent in hyper endemic areas for certain helminthic infections like fascioliasis with diverse kinds of herbivores as egg passer hosts.

  6. Brain MRI lesions in neuromyelitis optica: clinical case; Lesiones cerebrales de resonancia magnetica en neuromielitis optica: caso clinico

    Energy Technology Data Exchange (ETDEWEB)

    Rosales Bravo, Luis Guillermo; Heyden Cordero, Marvin; Chinchilla Weinstok, Dennis; Mendelewicz Goldwaig, Isaias, E-mail: neurologia.cima@gmail.com [Caja Costarricense del Seguro Social, Hospital Mexico, Div. de Neurologia, San Jose (Costa Rica)

    2011-10-15

    Many cases of patients with neuromyelitis optica have submitted without demyelinating lesions in the cerebral white matter, it has documented that this entity can cause from the onset of illness or through its natural evolution. Diagnostic methods currently as Magnetic Resonance Imaging (MRI) and specific antibodies in plasma (such as antiaquaporin-4) have been diagnosed of neuromyelitis optica cases that were initially confused with multiple sclerosis. Disease in Costa Rica has been little prevalent and is not exactly known what the prevalence and incidence. The degree of disorder is illustrated through a case study, both in the cerebral white matter as spinal cord, in a patient with neuromyelitis optica during a follow-up period of 4 years. This is the first case that has been reported in the scientific literature of Costa Rica. (author) [Spanish] Muchos casos de pacientes con neuromielitis optica se han presentado sin lesiones desmielinizantes en la sustancia blanca cerebral, se ha documentado que esta entidad puede causarlas desde el inicio de la enfermedad o a traves de su evolucion natural. Los metodos de diagnostico en la actualidad como la Imagen por Resonancia Magnetica (IRM) y la determinacion de anticuerpos especificos en plasma (como la antiaquaporina-4) han logrado diagnosticar algunos casos de neuromielitis optica que inicialmente fueron confundidos con esclerosis multiple. La enfermedad en Costa Rica ha sido poco prevalente y no se ha conocido con exactitud cual es la prevalencia e incidencia. El grado de afeccion ha sido ilustrado a traves de un caso clinico, tanto en la sustancia blanca cerebral como espinal, en un paciente con neuromielitis optica durante un periodo de seguimiento de 4 anos. Este es el primer caso que ha sido reportado en la literatura cientifica de Costa Rica. (autor)

  7. Evolution of novel wood decay mechanisms in Agaricales revealed by the genome sequences of Fistulina hepatica and Cylindrobasidium torrendii

    Science.gov (United States)

    Floudas, Dimitrios; Held, Benjamin W.; Riley, Robert; Nagy, Laszlo G.; Koehler, Gage; Ransdell, Anthony S.; Younus, Hina; Chow, Julianna; Chiniquy, Jennifer; Lipzen, Anna; Tritt, Andrew; Sun, Hui; Haridas, Sajeet; LaButti, Kurt; Ohm, Robin A.; Kües, Ursula; Blanchette, Robert A.; Grigoriev, Igor V.; Minto, Robert E.; Hibbett, David S.

    2015-01-01

    Wood decay mechanisms in Agaricomycotina have been traditionally separated in two categories termed white and brown rot. Recently the accuracy of such a dichotomy has been questioned. Here, we present the genome sequences of the white rot fungus Cylindrobasidium torrendii and the brown rot fungus Fistulina hepatica both members of Agaricales, combining comparative genomics and wood decay experiments. Cylindrobasidium torrendii is closely related to the white-rot root pathogen Armillaria mellea, while F. hepatica is related to Schizophyllum commune, which has been reported to cause white rot. Our results suggest that C. torrendii and S. commune are intermediate between white-rot and brown-rot fungi, but at the same time they show characteristics of decay that resembles soft rot. Both species cause weak wood decay and degrade all wood components but leave the middle lamella intact. Their gene content related to lignin degradation is reduced, similar to brown-rot fungi, but both have maintained a rich array of genes related to carbohydrate degradation, similar to white-rot fungi. These characteristics appear to have evolved from white-rot ancestors with stronger ligninolytic ability. Fistulina hepatica shows characteristics of brown rot both in terms of wood decay genes found in its genome and the decay that it causes. However, genes related to cellulose degradation are still present, which is a plesiomorphic characteristic shared with its white-rot ancestors. Four wood degradation-related genes, homologs of which are frequently lost in brown-rot fungi, show signs of pseudogenization in the genome of F. hepatica. These results suggest that transition towards a brown rot lifestyle could be an ongoing process in F. hepatica. Our results reinforce the idea that wood decay mechanisms are more diverse than initially thought and that the dichotomous separation of wood decay mechanisms in Agaricomycotina into white rot and brown rot should be revisited. PMID:25683379

  8. Magnetic losses and reactance change by the use of metallic conduit in electrical installations; Perdidas magneticas y cambio de reactancia por la tuberia metalica en instalaciones electricas

    Energy Technology Data Exchange (ETDEWEB)

    Campero Littlewood, Eduardo; Castaneda D, Miguel; Castulo A, Roberto; Bratu Serban, Neagu [Universidad Autonoma Metropolitana-Azcapotzalco, Mexico, D. F. (Mexico)

    1996-12-31

    In this paper are presented the results obtained in the measurement of magnetic losses in metallic conduits in electric installations. The losses for parasitic currents and by hysteresis were obtained in different conditions of magnetic density, caused by the net current resulting from the sum of all the currents of all the conductors that travel along the conduit. These currents are named differential currents, and the conditions in which they appear in the electric installations are described. The magnetic induction phenomenon that occurs in metallic conduit is briefly described and the basic concepts that describe it. A theoretical-experimental methodology is offered to quantify the energy losses by parasitic currents and by the metal magnetization in these metallic conduits. At the same time, the impact of these differential currents impact in the reactance of the electric installation is quantified. The obtained results for twelve conduits of different diameters and different wall thickness are shown. A comparison analysis is made with the reported results in a previous article, where the losses were estimated by means of the temperature rise measurement of the metallic wall. [Espanol] En este articulo se presentan los resultados obtenidos en la medicion de perdidas magneticas en tuberias conduit (metalicas) en instalaciones electricas. Las perdidas por corrientes parasitas y por histeresis se obtuvieron para diferentes condiciones de densidad magnetica, provocada por la corriente neta resultante de la suma de las corrientes de todos los conductores que viajan por la tuberia. A estas corrientes se les designa corrientes diferenciales y se describen las condiciones en las que estas aparecen en las instalaciones electricas. Se explica brevemente el fenomeno de induccion magnetica que se presenta en las tuberias metalicas y los conceptos basicos que la describen. Se plantea una metodologia teorico-experiemental para cuantificar las perdidas de energia, por

  9. Magnetic losses and reactance change by the use of metallic conduit in electrical installations; Perdidas magneticas y cambio de reactancia por la tuberia metalica en instalaciones electricas

    Energy Technology Data Exchange (ETDEWEB)

    Campero Littlewood, Eduardo; Castaneda D, Miguel; Castulo A, Roberto; Bratu Serban, Neagu [Universidad Autonoma Metropolitana-Azcapotzalco, Mexico, D. F. (Mexico)

    1997-12-31

    In this paper are presented the results obtained in the measurement of magnetic losses in metallic conduits in electric installations. The losses for parasitic currents and by hysteresis were obtained in different conditions of magnetic density, caused by the net current resulting from the sum of all the currents of all the conductors that travel along the conduit. These currents are named differential currents, and the conditions in which they appear in the electric installations are described. The magnetic induction phenomenon that occurs in metallic conduit is briefly described and the basic concepts that describe it. A theoretical-experimental methodology is offered to quantify the energy losses by parasitic currents and by the metal magnetization in these metallic conduits. At the same time, the impact of these differential currents impact in the reactance of the electric installation is quantified. The obtained results for twelve conduits of different diameters and different wall thickness are shown. A comparison analysis is made with the reported results in a previous article, where the losses were estimated by means of the temperature rise measurement of the metallic wall. [Espanol] En este articulo se presentan los resultados obtenidos en la medicion de perdidas magneticas en tuberias conduit (metalicas) en instalaciones electricas. Las perdidas por corrientes parasitas y por histeresis se obtuvieron para diferentes condiciones de densidad magnetica, provocada por la corriente neta resultante de la suma de las corrientes de todos los conductores que viajan por la tuberia. A estas corrientes se les designa corrientes diferenciales y se describen las condiciones en las que estas aparecen en las instalaciones electricas. Se explica brevemente el fenomeno de induccion magnetica que se presenta en las tuberias metalicas y los conceptos basicos que la describen. Se plantea una metodologia teorico-experiemental para cuantificar las perdidas de energia, por

  10. Seroepidemiology of Fasciola Hepatica in Mersin province and surrounding towns and the role of family history of the Fascioliasis in the transmission of the parasite.

    Science.gov (United States)

    Ozturhan, Hakan; Emekdaş, Gürol; Sezgin, Orhan; Korkmaz, Metin; Altintaş, Engin

    2009-09-01

    Fascioliasis is an important zoonotic disease caused by Fasciola hepatica. This zoonosis may cause serious morbidity and a considerable financial burden. Knowledge about Fasciola hepatica and interest in this parasite have increased in Turkey recently. However, there have been few studies on the real prevalence of this condition in the country. Therefore, we aimed to determine the prevalence of fascioliasis and the role of family history of the condition in the transmission of the parasite in the province of Mersin. Taking account of their populations, 729 people without a family history of fascioliasis and 155 people with a family history of fascioliasis from the city of Mersin and randomly selected three towns were included into the study to obtain a sample that well represented the population of the province of Mersin. A questionnaire composed of items about consumption of green leafy vegetables, stock-breeding and clinical symptoms of the disease was used to collect data. Excretory/ secretory (ES)-ELISA was used to detect IgG antibodies to Fasciola hepatica. People seropositive for Fasciola hepatica underwent abdominal ultrasonography, physical examination, biochemistry, and stool tests for the detection of Fasciola hepatica eggs. A total of 0.79% of the participants were seropositive for Fasciola hepatica. One point ninety-three percent of the individuals with a family history of fascioliasis and 0.55% of the individuals without a family history of fascioliasis were seropositive for Fasciola hepatica. Out of 7 individuals found to be seropositive for Fasciola hepatica, 5 were female, 2 were male, and 4 had a family history of fascioliasis. Five and 4 patients, respectively, had a history of consuming green leafy vegetables and 4 had a history of stock-breeding. The clinical evaluation revealed that 4 patients had at least one sign of fascioliasis. Three patients had signs of fascioliasis on ultrasonography and 1 had Fasciola hepatica egg in stool examination

  11. Little genetic variability in resilience among cattle exists for a range of performance traits across herds in Ireland differing in Fasciola hepatica prevalence.

    Science.gov (United States)

    Twomey, Alan J; Graham, David A; Doherty, Michael L; Blom, Astrid; Berry, Donagh P

    2018-06-04

    It is anticipated that in the future, livestock will be exposed to a greater risk of infection from parasitic diseases. Therefore, future breeding strategies for livestock, which are generally long-term strategies for change, should target animals adaptable to environments with a high parasitic load. Covariance components were estimated in the present study for a selection of dairy and beef performance traits over herd-years differing in Fasciola hepatica load using random regression sire models. Herd-year prevalence of F. hepatica was determined by using F. hepatica-damaged liver phenotypes which were recorded in abattoirs nationally. The data analyzed consisted up to 83,821 lactation records from dairy cows for a range of milk production and fertility traits, as well as 105,054 young animals with carcass-related information obtained at slaughter. Reaction norms for individual sires were derived from the random regression coefficients. The heritability and additive genetic standard deviations for all traits analyzed remained relatively constant as herd-year F. hepatica prevalence gradient increased up to a prevalence level of 0.7; although there was a large increase in heritability and additive genetic standard deviation for milk and fertility traits in the observed F. hepatica prevalence levels >0.7, only 5% of the data existed in herd-year prevalence levels >0.7. Very little rescaling, therefore, exists across differing herd-year F. hepatica prevalence levels. Within-trait genetic correlations among the performance traits across different herd-year F. hepatica prevalence levels were less than unity for all traits. Nevertheless, within-trait genetic correlations for milk production and carcass traits were all >0.8 for F. hepatica prevalence levels between 0.2 and 0.8. The lowest estimate of within-trait genetic correlations for the different fertility traits ranged from -0.03 (SE = 1.09) in age of first calving to 0.54 (SE = 0.22) for calving to first service

  12. Identification of Chalcones as Fasciola hepatica Cathepsin L Inhibitors Using a Comprehensive Experimental and Computational Approach.

    Directory of Open Access Journals (Sweden)

    Florencia Ferraro

    2016-07-01

    Full Text Available Increased reports of human infections have led fasciolosis, a widespread disease of cattle and sheep caused by the liver flukes Fasciola hepatica and Fasciola gigantica, to be considered an emerging zoonotic disease. Chemotherapy is the main control measure available, and triclabendazole is the preferred drug since is effective against both juvenile and mature parasites. However, resistance to triclabendazole has been reported in several countries urging the search of new chemical entities and target molecules to control fluke infections.We searched a library of forty flavonoid derivatives for inhibitors of key stage specific Fasciola hepatica cysteine proteases (FhCL3 and FhCL1. Chalcones substituted with phenyl and naphtyl groups emerged as good cathepsin L inhibitors, interacting more frequently with two putative binding sites within the active site cleft of the enzymes. One of the compounds, C34, tightly bounds to juvenile specific FhCL3 with an IC50 of 5.6 μM. We demonstrated that C34 is a slow-reversible inhibitor that interacts with the Cys-His catalytic dyad and key S2 and S3 pocket residues, determinants of the substrate specificity of this family of cysteine proteases. Interestingly, C34 induces a reduction in NEJ ability to migrate through the gut wall and a loss of motility phenotype that leads to NEJ death within a week in vitro, while it is not cytotoxic to bovine cells.Up to date there are no reports of in vitro screening for non-peptidic inhibitors of Fasciola hepatica cathepsins, while in general these are considered as the best strategy for in vivo inhibition. We have identified chalcones as novel inhibitors of the two main Cathepsins secreted by juvenile and adult liver flukes. Interestingly, one compound (C34 is highly active towards the juvenile enzyme reducing larval ability to penetrate the gut wall and decreasing NEJ´s viability in vitro. These findings open new avenues for the development of novel agents to control

  13. Towards assessing fine-scale indicators for the spatial transmission risk of Fasciola hepatica in cattle

    Directory of Open Access Journals (Sweden)

    Johannes Charlier

    2011-05-01

    Full Text Available In order to improve the spatial resolution of current risk maps for fasciolosis in cattle, more knowledge is needed with respect to farm-level factors that determine infection risk. In this study, we visited 39 dairy farms within a predefined low- and high-risk area for fasciolosis in Belgium and assessed their infection status by an indirect bulk tank milk enzyme-linked immunosorbent assay (ELISA. Management factors were collected and all pastured lands of the farms were visited to identify and georeference potential snail habitats. The habitats were visually characterised, investigated for the presence of the intermediate host snails of Fasciola hepatica (i.e.Galba truncatula and Radix spp. and used in a geographical information system (GIS to construct overlays including information on soil and hydrology. A linear regression model was used to evaluate associations between bulk tank milk ELISA results and farm level management and habitat factors. A logistic, mixed model was used to identify possible risk factors for the presence of intermediate host snails on a potential habitat. Potential snail habitats were found in 35 out of 39 farms. A total of 87 potential habitats were identified and on 29% of these, intermediate host snails were found. The number of potential habitats, the presence of snails, drainage of pastures, month of turnout of the cows, stocking rate, type of watering place and risk area were significantly associated with the bulk tank milk ELISA result and explained 85% of the observed variation. Intermediate host snails were more likely to be present with increasing surface of the potential habitat and on loamy soils. This study confirms the importance of farm management factors in the infection risk for F. hepatica in cattle and highlights that the combination of management factors with characterization of snail habitats is a powerful means to predict the infection risk with F. hepatica at the individual farm level. Further

  14. Epidemiological studies on Fasciola hepatica in Gafsa oases (South West of Tunisia

    Directory of Open Access Journals (Sweden)

    Hammami H.

    2007-09-01

    Full Text Available Epidemiological investigations on Fasciola hepatica fasciolasis were carried out from July 2004 to June 2005 in the Gafsa oases (Tunisia after the detection of a human case. Three habitats were studied: one in El Gsar and two in Ain Soltan. The prevalence of human infection was 6.6 %. The presence of the parasite was detected through serology in 14.3 % of cattle, 35 % of sheep and 68.4 % of goats. The plants Apium nodiflorum, Oxalis cernua and Sonchus maritimus were suspected to be at the origin of animal contamination and Apium nodiflorum was incriminated in human infection. The prevalence of the infection of the intermediate host Galba truncatula (G. truncatula was 19.2 % from July 2004 to June 2005. Gafsa oases constitute a new location for the development of fasciolasis in the southern west of Tunisia.

  15. Fasciola Hepatica Mimicking Malignancy on 18F-Fluorodeoxyglucose-Positron Emission Tomography/Computed Tomography

    Science.gov (United States)

    Sürücü, Erdem; Demir, Yusuf; Dülger, Ahmet C.; Batur, Abdüssamed; Ölmez, Şehmus; Kitapçı, Mehmet T.

    2016-01-01

    A 48-year-old female with complaints of gastrointestinal symptoms such as abdominal pain, fatigue, vomiting, nausea, and weight loss was diagnosed with neuroendocrine tumor after removal of a 2 mm lesion from the stomach with endoscopic biopsy. Her magnetic resonance imaging that was performed due to on-going symptoms showed multiple linear hypointense lesions in the liver. Positron emission tomography/computed tomography (PET/CT) scan was performed for differential diagnosis, which showed high fluorodeoxyglucose (FDG) uptake in these lesions. Clinical and laboratory findings revealed the final diagnosis as Fasciola hepatica. The imaging features of this case is presented to aid in differentiating this infectious disease from malignancy and avoid misdiagnosis on FDG-PET/CT. PMID:27751978

  16. Burden of Fasciola hepatica Infection among children from Paucartambo in Cusco, Peru.

    Science.gov (United States)

    Lopez, Martha; White, A Clinton; Cabada, Miguel M

    2012-03-01

    There is a high prevalence of fascioliasis in the Peruvian highlands, but most cases remain undiagnosed. The burden of disease caused by chronic subclinical infection is largely unknown. We studied school-age children from a district in Paucartambo Province in Cusco, Peru to evaluate the burden of disease caused by subclinical fascioliasis. Parasite eggs and/or larvae were identified in 46.2% of subjects, including Fasciola hepatica in 10.3% of subjects. Fascioliasis was independently associated with anemia (adjusted odds ratio = 3.01 [1.10-8.23]). Subclinical fascioliasis was common among children and strongly associated with anemia. Anemia should be recognized as an important component of the burden of disease from fascioliasis.

  17. Fasciola Hepatica Mimicking Malignancy on 18F-Fluorodeoxyglucose-Positron Emission Tomography/Computed Tomography

    Directory of Open Access Journals (Sweden)

    Erdem Sürücü

    2016-10-01

    Full Text Available A 48-year-old female with complaints of gastrointestinal symptoms such as abdominal pain, fatigue, vomiting, nausea, and weight loss was diagnosed with neuroendocrine tumor after removal of a 2 mm lesion from the stomach with endoscopic biopsy. Her magnetic resonance imaging that was performed due to on-going symptoms showed multiple linear hypointense lesions in the liver. Positron emission tomography/computed tomography (PET/CT scan was performed for differential diagnosis, which showed high fluorodeoxyglucose (FDG uptake in these lesions. Clinical and laboratory findings revealed the final diagnosis as Fasciola hepatica. The imaging features of this case is presented to aid in differentiating this infectious disease from malignancy and avoid misdiagnosis on FDG-PET/CT.

  18. Were natural forms of treatment for fasciola hepatica available to the Etruscans?

    DEFF Research Database (Denmark)

    Harrison, Adrian Paul; Turfa, Jean M.

    2010-01-01

    , that can be used to heal, to cure or to prevent infection and disease. Some tantalizing hints of herbal folk-medicine may be discerned in the rare remnants of literary and archaeological evidence for the Etruscan culture, a distinctive group who dominated central Italy through the first half of the first......In the very distant past, European and Mediterranean peoples must have shared an extensive collection of folk experiences as to how certain plants were to be used as medicines. Plants comprise active secondary metabolites such as essential oils, alkaloids, bitters, flavonoids, tanins and glycosides....... This paper investigates documentation for the plants known to the Etruscans, focusing particularly on those natural forms of treatment that would have been efficaceous in terms of Fasciola hepatica (Liver fluke) infection. Interestingly, some of the plants in the putative ancient Etruscan herbal remain...

  19. Burden of Fasciola hepatica Infection among Children from Paucartambo in Cusco, Peru

    Science.gov (United States)

    Lopez, Martha; White, A. Clinton; Cabada, Miguel M.

    2012-01-01

    There is a high prevalence of fascioliasis in the Peruvian highlands, but most cases remain undiagnosed. The burden of disease caused by chronic subclinical infection is largely unknown. We studied school-age children from a district in Paucartambo Province in Cusco, Peru to evaluate the burden of disease caused by subclinical fascioliasis. Parasite eggs and/or larvae were identified in 46.2% of subjects, including Fasciola hepatica in 10.3% of subjects. Fascioliasis was independently associated with anemia (adjusted odds ratio = 3.01 [1.10–8.23]). Subclinical fascioliasis was common among children and strongly associated with anemia. Anemia should be recognized as an important component of the burden of disease from fascioliasis. PMID:22403322

  20. Molecular detection of Fasciola hepatica in water sources of District Nowshehra Khyber Pakhtunkhwa Pakistan

    Science.gov (United States)

    Khan, Imran; Khan, Amir Muhammad; Ayaz, Khan, Sanaullah; Anees, Muhammad; Khan, Shaukat Ali

    2012-12-01

    Fascioliasis is spread through contamination of water sources and cause morbidity throughout the world. In the current study 300 water samples were processed by PCR for detection of Fasciola hepatica. The overall prevalence in different water sources was 9.66 % (29/300). Highest prevalence was recorded in drain water16 % (16/100) followed by tube well water 10% (4/40), open well water 8 % (8/100) and the lowest was recorded in tap water 1.66 %(1/60). The significant difference P < 0.05 was recorded during data analysis. The highest prevalence was recorded in summer. It was concluded from the study that cleaning and filtration should be adopted to avoid the health hazards against water borne zoonotic parasites.

  1. Viabilidade de ovos de Fasciola hepatica de bovinos em sistema de biodigestão anaeróbia Viability of bovine's Fasciola hepatica eggs in a system of anaerobic biodigestion

    Directory of Open Access Journals (Sweden)

    M.B. Mentz

    2004-08-01

    Full Text Available Cattle Fasciola hepatica egg-viability was studied in closed system of anaerobic biodigestion. Two thirds of a biodigestor were filled with liquefied manure from eight Hosltein cows, naturally infected. For 10 consecutive weeks of observation, the biodigestor internal temperature ranged from 24ºC to 28ºC, and external temperature from 26ºC to 33ºC. All the effluent samples showed a constant pH of 7. The samples of the effluent were weekly collected, in a total of 10 and submitted to techniques to detect the presence and viability of the trematode eggs. It was detected a 42-day egg viability. Therefore, liquefied cattle manure containing Fasciola hepatica eggs must remain under anaerobic biodigestion for at least 42 days before its safe return to the environment.

  2. Detection and genetic distance of resistant populations of Pseudosuccinea columella (Mollusca: Lymnaeidae) to Fasciola hepatica (Trematoda: Digenea) using RAPD markers.

    Science.gov (United States)

    Calienes, Aymé Fernandez; Fraga, Jorge; Pointier, Jean-Pierre; Yong, Mary; Sanchez, Jorge; Coustau, Christine; Gutiérrez, Alfredo; Théron, André

    2004-09-01

    Twelve natural populations of Pseudosuccinea columella snails, sampled in the western and central regions of Cuba, were analyzed using the RAPD-PCR technique to screen for resistance to Fasciola hepatica. Ten OPA primers previously shown to produce marker bands for resistance and susceptibility were tested. A new population of P. columella (El Azufre, Pinar del Río) exhibited the amplification patterns of resistant snails, and its resistant status was confirmed after experimental exposure to miracidia. No genetic variability was detected across or within the susceptible isolates. Similarly, the novel resistant isolate displayed an RAPD profile identical to the profile of two other isolates previously identified as resistant to F. hepatica. However, clear differences in RAPD banding patterns and genetic distance were observed between resistant and susceptible isolates.

  3. Time-course investigation of the gene expression profile during Fasciola hepatica infection: A microarray-based study

    Directory of Open Access Journals (Sweden)

    Jose Rojas-Caraballo

    2015-12-01

    Full Text Available Fasciolosis is listed as one of the most important neglected tropical diseases according with the World Health Organization and is also considered as a reemerging disease in the human beings. Despite there are several studies describing the immune response induced by Fasciola hepatica in the mammalian host, investigations aimed at identifying the expression profile of genes involved in inducing hepatic injury are currently scarce. Data presented here belong to a time-course investigation of the gene expression profile in the liver of BALB/c mice infected with F. hepatica metacercariae at 7 and 21 days after experimental infection. The data published here have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE69588, previously published by Rojas-Caraballo et al. (2015 in PLoS One [1].

  4. Ovicidal effect of the methanolic extract of ginger (Zingiber officinale) on Fasciola hepatica eggs: an in vitro study.

    Science.gov (United States)

    Moazeni, Mohammad; Khademolhoseini, Ali Asghar

    2016-09-01

    Fasciolosis is of considerable economic and public health importance worldwide. Little information is available on the ovicidal effects of anthelminthic drugs. The use of ovicidal anthelmintics can be effective in disease control. In this study, the effectiveness of the methanolic extract of ginger (Zingiber officinale) on the eggs of Fasciola hepatica is investigated. Fasciola hepatica eggs were obtained from the gall bladders of naturally infected sheep and kept at 4 °C until use. The eggs were exposed to varying concentrations of ginger extract (1, 5, 10, 25 and 50 mg/mL) for 24, 48 and 72 h. To investigate the effect of the ginger extracts on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that F. hepatica eggs are susceptible to the methanolic extract of Z. officinale. The ovicidal effect of ginger extract at a concentration of 1 mg/mL with 24, 48 and 72 h treatment time was 46.08, 51.53 and 69.09 % respectively (compared with 22.70 % for control group). The ovicidal effect of ginger extract at a concentration of 5 mg/mL after 24 h was 98.84 %. One hundred percent ovicidal efficacy was obtained through application of ginger extract at concentrations of 5 and 10 mg/mL with a 48 and 24 h treatment time respectively. The in vitro ovicidal effect of the methanolic extract of Z. officinale was satisfactory in this study, however, in vivo efficacy of this extract, remains for further investigation. To the best of our knowledge, this is the first report on the ovicidal effect of Z. officinale against F. hepatica eggs.

  5. Assessment of flukicide efficacy against Fasciola hepatica in sheep in Sweden in the absence of a standardised test.

    Science.gov (United States)

    Novobilský, Adam; Amaya Solis, Natalia; Skarin, Moa; Höglund, Johan

    2016-12-01

    Anthelmintic resistance (AR) to Fasciola hepatica is emerging worldwide. Recently, AR to the adulticide compound albendazole (ABZ) was shown in Argentina and Spain. In Sweden, ABZ treatment failure against F. hepatica was first reported in sheep in 2012. The present study tested the efficacy of ABZ and triclabendazole (TCBZ) in sheep naturally infected with F. hepatica using a combination of three different diagnostic methods: faecal egg counts (FEC), coproantigen ELISA (cELISA) and Fasciola egg hatch test (FEHT). Two deworming trials, in November 2014 and January 2015, were performed on two sheep farms (farms A and B) in south-western Sweden. Except ABZ in November, treatment with ABZ or TCBZ achieved sufficient efficacy (97-100%) against adult F. hepatica on farm A. In contrast, ABZ treatment failed in the sheep flock on farm B, despite low initial faecal egg output. On farm B, ABZ efficacy based on FEC was 67% (95% CI: 35-84) and four of eight ewes tested were coproantigen-positive 21 days post-treatment. Ovicidal activity of ABZ against Fasciola eggs in isolates from both farms and one additional bovine isolate were tested by FEHT to exclude the presence of juvenile flukes and other factors such as dosing failure and poor quality of drug product. Irrespective of drug trial, data from FEHT showed significantly lower ovicidal activity of ABZ for the ovine farm B isolate than for the isolate from farm A. This confirms that the low efficacy of ABZ in sheep flock B was associated with ABZ resistance. Overall, the usefulness of three complementary methods for detection of ABZ resistance in the field was demonstrated. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  6. Comparative assessment of recombinant and native immunogenic forms of Fasciola hepatica proteins for serodiagnosis of sheep fasciolosis.

    Science.gov (United States)

    Mokhtarian, Kobra; Meamar, Ahmad Reza; Khoshmirsafa, Majid; Razmjou, Elham; Masoori, Leila; Khanmohammadi, Majid; Akhlaghi, Lame; Falak, Reza

    2018-01-01

    Laboratory diagnosis of sheep fasciolosis is commonly performed by coprological examinations; however, this method may lead to false negative results during the acute phase of the infection. Furthermore, the poor sensitivity of coprological methods is considered to be a paradox in the chronic phase of the infection. In this study, we compared the immunoreactivity of native and recombinant forms of Fasciola hepatica excretory/secretory antigens and determined their capabilities for the development of F. hepatica-specific immunoassays. Immunoreactivity and specificity of recombinant and native forms of F. hepatica antigens, including fatty acid binding protein (FABP), glutathione-S-transferase (GST), and cathepsin L-1 (CL1), in parallel with native forms of FABP and GST, were studied for serodiagnosis of the chronic form of sheep fasciolosis, individually or in combination with each other by enzyme-linked immunosorbent assays (ELISA). The correlation of the findings was assessed by receiver-operator characteristic (ROC); furthermore, the specificity and sensitivity were assessed by Youden's J. Serologic cross-reactivity was evaluated using samples from healthy sheep (n = 40), Fasciola-infected sheep (n = 30), and sheep with other parasitic infections (n = 43). The FABPs were determined to be greater than 95% sensitive for F. hepatica serodiagnosis. The most desirable diagnostic recombinant antigen was rCL1, which showed 100% sensitivity and 97% specificity in ELISA and was capable of discriminating the positive and negative samples by maximum Youden's J results. We conclude that rCL1 can be used for routine serodiagnosis of chronic fasciolosis. Thus, it could be advantageous in development of immunoassays for screening of ovine herds in fasciolosis-endemic areas and as a reliable agent for detection of fasciolosis in non-endemic regions.

  7. A mucin-like peptide from Fasciola hepatica induces parasite-specific Th1-type cell immunity.

    Science.gov (United States)

    Noya, Verónica; Brossard, Natalie; Berasaín, Patricia; Rodríguez, Ernesto; Chiale, Carolina; Mazal, Daniel; Carmona, Carlos; Freire, Teresa

    2016-03-01

    Fasciolosis, caused by the liver fluke Fasciola hepatica, is a major parasitic disease of livestock that causes significant economic losses worldwide. Although drugs are effective against liver flukes, they do not prevent reinfection, and continuous treatment is costly. Moreover, resistant fluke strains are emerging. In this context, vaccination is a good alternative since it provides a cost-effective long-term prevention strategy to control fasciolosis. In this paper, we evaluate the Fhmuc peptide as a potential vaccine against fasciolosis. This peptide derives from a mucin-like protein highly expressed in the infective stage of Fasciola hepatica. Mucin-like molecules expressed by parasites can contribute to several infection processes by protecting the parasite from host proteases and recognition by the immune system. We show that the Fhmuc peptide induces Th1-like immune responses specific for F. hepatica excretion-secretion products (FhESP) with a high production of IFNγ. We also investigated whether this peptide could protect animals from infection, and present preliminary data indicating that animals treated with Fhmuc exhibited reduced liver damage compared to non-immunised animals and that this protection was associated with a recruitment of B and T lymphocytes in the peritoneum, as well as eosinophils and mature dendritic cells. These results suggest that the mucin-like peptide Fhmuc could constitute a potential vaccine candidate against fasciolosis and pave the way towards the development of vaccines against parasites.

  8. Prevalence and Molecular Diagnosis of Fasciola hepatica in Sheep and Goats in Different Districts of Punjab, Pakistan

    Directory of Open Access Journals (Sweden)

    Waseem Shahzad*, Khalid Mehmood1, Rashid Munir2, Waqar Aslam, Muhammad Ijaz1, Rashid Ahmad, Mohammad Sarwar Khan1 and Ahmad Jawad Sabir1

    2012-10-01

    Full Text Available A study was conducted to determine the prevalence and molecular diagnosis of fasciolosis in Lohi sheep and Beetal goats in Okara, Sahiwal and Lahore districts of Punjab province. A total of 800 samples including 200 fecal and 200 bile, each from Lohi sheep and Beetal goats were collected and processed for microscopic examination of Fasciola eggs as well as its confirmation through polymerase chain reaction (PCR. Of the fecal samples, 3.5 and 2% samples whereas from bile samples, 6.5 and 4% bile samples from sheep and goats were found microscopically positive for Fasciola hepatica, respectively. By PCR, 4.5 and 3.5% fecal samples and 8.5 and 5% bile samples from sheep and goats, respectively were confirmed for F. hepatica. Statistically sheep and goats species were found equally likely acquiring the F. hepatica diagnosed by either source of material (fecal and bile samples similarly fecal and bile samples were equally likely showing fasciolosis in either of sheep and goats.

  9. Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China.

    Science.gov (United States)

    Ichikawa-Seki, Madoka; Peng, Mao; Hayashi, Kei; Shoriki, Takuya; Mohanta, Uday Kumar; Shibahara, Toshiyuki; Itagaki, Tadashi

    2017-02-01

    The well-known pathogens of fasciolosis, Fasciola hepatica (Fh) and Fasciola Gigantica (Fg), possess abundant mature sperms in their seminal vesicles, and thus, they reproduce bisexually. On the other hand, aspermic Fasciola flukes reported from Asian countries, which have no sperm in their seminal vesicles, probably reproduce parthenogenetically. The aim of this study was to reveal the origin of aspermic Fasciola flukes. The nuclear single copy markers, phosphoenolpyruvate carboxykinase and DNA polymerase delta, were employed for analysis of Fasciola species from China. The hybrid origin of aspermic Fasciola flukes was strongly suggested by the presence of the Fh/Fg type, which includes DNA fragments of both F. hepatica and F. gigantica. China can be regarded as the cradle of the interspecific hybridization because F. hepatica and F. gigantica were detected in the northern and southern parts of China, respectively, and hybrids flukes were distributed between the habitats of the two species. The Chinese origin was supported by the fact that a larger number of mitochondrial NADH dehydrogenase subunit 1 (nad1) haplotypes was detected in Chinese aspermic Fasciola populations than in aspermic populations from the neighbouring countries. Hereafter, 'aspermic' Fasciola flukes should be termed as 'hybrid' Fasciola flukes.

  10. Six-year longitudinal study of Fasciola hepatica bulk milk antibody ELISA in the dairy dense region of the Republic Ireland.

    Science.gov (United States)

    Munita, M P; Rea, R; Bloemhoff, Y; Byrne, N; Martinez-Ibeas, A M; Sayers, R G

    2016-11-01

    Completion of the F. hepatica lifecycle is dependent on suitable climatic conditions for development of immature stages of the parasite, and its snail intermediate host. Few investigations have been conducted regarding temporal variations in F. hepatica status in Irish dairy herds. The current study aimed to conduct a longitudinal study examining annual and seasonal trends in bulk milk seropositivity over six years, while also investigating associations with soil temperature, rainfall and flukicide treatment. Monthly bulk milk samples (BTM) were submitted by 28 herds between March 2009 and December 2014. In all, 1337 samples were analysed using a Cathepsin L1 ELISA. Soil temperature, rainfall and management data were obtained for general estimating equation and regression analyses. A general decrease in milk seropositivity was observed over the six year study period and was associated with an increased likelihood of treating for liver fluke (OR range=2.73-6.96). Annual and seasonal analyses of rainfall and F. hepatica BTM status yielded conflicting results. Higher annual rainfall (>1150mm) yielded a lower likelihood of being BTM positive than annual rainfall of hepatica in wetter years, although a 'wash effect' by high rainfall of the free living stages and snails cannot be ruled out. Higher seasonal rainfall (>120mm), however, was associated with increased ELISA S/P% values (Coefficient=9.63S/P%; P=0.001). Soil temperature was not found to influence F. hepatica to the same extent as rainfall and may reflect the lack of severe temperature fluctuations in Ireland. Flukicides active against both immature and mature F. hepatica were approximately half as likely to record a positive F. hepatica herd BTM status than a flukicide active against only the mature stage of the parasite (OR≅0.45; Phepatica data, which can vary significantly. Additionally, it highlights the progress that can be achieved in fluke control by application of a continuous BTM monitoring program

  11. Relative importance of management, meteorological and environmental factors in the spatial distribution of Fasciola hepatica in dairy cattle in a temperate climate zone.

    Science.gov (United States)

    Bennema, S C; Ducheyne, E; Vercruysse, J; Claerebout, E; Hendrickx, G; Charlier, J

    2011-02-01

    Fasciola hepatica, a trematode parasite with a worldwide distribution, is the cause of important production losses in the dairy industry. Diagnosis is hampered by the fact that the infection is mostly subclinical. To increase awareness and develop regionally adapted control methods, knowledge on the spatial distribution of economically important infection levels is needed. Previous studies modelling the spatial distribution of F. hepatica are mostly based on single cross-sectional samplings and have focussed on climatic and environmental factors, often ignoring management factors. This study investigated the associations between management, climatic and environmental factors affecting the spatial distribution of infection with F. hepatica in dairy herds in a temperate climate zone (Flanders, Belgium) over three consecutive years. A bulk-tank milk antibody ELISA was used to measure F. hepatica infection levels in a random sample of 1762 dairy herds in the autumns of 2006, 2007 and 2008. The infection levels were included in a Geographic Information System together with meteorological, environmental and management parameters. Logistic regression models were used to determine associations between possible risk factors and infection levels. The prevalence and spatial distribution of F. hepatica was relatively stable, with small interannual differences in prevalence and location of clusters. The logistic regression model based on both management and climatic/environmental factors included the factors: annual rainfall, mowing of pastures, proportion of grazed grass in the diet and length of grazing season as significant predictors and described the spatial distribution of F. hepatica better than the model based on climatic/environmental factors only (annual rainfall, elevation and slope, soil type), with an Area Under the Curve of the Receiver Operating Characteristic of 0.68 compared with 0.62. The results indicate that in temperate climate zones without large climatic

  12. Ocorrência de infecção natural de Fasciola hepatica Linnaeus, 1758 em Lymnaea columella Say, 1817 no Vale do Paraíba, SP, Brasil Natural infection by Fasciola hepatica in Lymnaea columella in the Paraíba river valley, S. Paulo, Brazil

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    Marlene Tiduko Ueta

    1980-06-01

    Full Text Available Foram registradas em Piquete, no vale do rio Paraíba do Sul (SP, Brasil, taxas de 1,22% e 0,14% de infecção natural em Lymnaea columella, por Fasciola hepatica. Em um único exemplar de Lymnaea columella dentre os 1.052 examinados, foram observadas rédias com xifidiocercárias, rédias com cercárias de Fasciola hepatica e metacercárias de Echinostomatidae.Infection rates of 1.22% and 0.14% were obtained in Lymnaea columella snails naturally infected by Fasciola hepatica. Samples of the snails were collected in Piquete, a municipality of Paraíba do Sul, a river valley area in the State of S. Paulo. Also observed was one of the 1052 specimen of the Lymnaea columella rediae which had xiphidiocercariae and rediae with Fasciola hepatica cercariae and metacercariae of Echinostomatidae.

  13. Adaptation of Lymnaea fuscus and Radix balthica to Fasciola hepatica through the experimental infection of several successive snail generations

    Science.gov (United States)

    2014-01-01

    Background High prevalence of Fasciola hepatica infection (>70%) was noted during several outbreaks before the 2000s in several French farms where Galba truncatula is lacking. Other lymnaeids such as Lymnaea fuscus, L. glabra and/or Radix balthica are living in meadows around these farms but only juvenile snails can sustain complete larval development of F. hepatica while older snails were resistant. The low prevalence of infection (<20%) and limited cercarial production (<50 cercariae per infected snail) noted with these juveniles could not explain the high values noted in these cattle herds. As paramphistomosis due to Calicophoron daubneyi was not still noted in these farms, the existence of another mode of infection was hypothesized. Experimental infection of several successive generations of L. glabra, originating from eggs laid by their parents already infected with this parasite resulted in a progressive increase in prevalence of snail infection and the number of shed cercariae. The aim of this paper was to determine if this mode of snail infection was specific to L. glabra, or it might occur in other lymnaeid species such as L. fuscus and R. balthica. Methods Five successive generations of L. fuscus and R. balthica were subjected to individual bimiracidial infections in the laboratory. Resulting rediae and cercariae in the first four generations were counted after snail dissection at day 50 p.e. (20°C), while the dynamics of cercarial shedding was followed in the F5 generation. Results In the first experiment, prevalence and intensity of F. hepatica infection in snails progressively increased from the F1 (R. balthica) or F2 (L. fuscus) generation. In the second experiment, the prevalence of F. hepatica infection and the number of shed cercariae were significantly lower in L. fuscus and R. balthica (without significant differences between both lymnaeids) than in G. truncatula. Conclusion The F. hepatica infection of several successive snail generations

  14. Development of functional genomic tools in trematodes: RNA interference and luciferase reporter gene activity in Fasciola hepatica.

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    Gabriel Rinaldi

    2008-07-01

    Full Text Available The growing availability of sequence information from diverse parasites through genomic and transcriptomic projects offer new opportunities for the identification of key mediators in the parasite-host interaction. Functional genomics approaches and methods for the manipulation of genes are essential tools for deciphering the roles of genes and to identify new intervention targets in parasites. Exciting advances in functional genomics for parasitic helminths are starting to occur, with transgene expression and RNA interference (RNAi reported in several species of nematodes, but the area is still in its infancy in flatworms, with reports in just three species. While advancing in model organisms, there is a need to rapidly extend these technologies to other parasites responsible for several chronic diseases of humans and cattle. In order to extend these approaches to less well studied parasitic worms, we developed a test method for the presence of a viable RNAi pathway by silencing the exogenous reporter gene, firefly luciferase (fLUC. We established the method in the human blood fluke Schistosoma mansoni and then confirmed its utility in the liver fluke Fasciola hepatica. We transformed newly excysted juveniles of F. hepatica by electroporation with mRNA of fLUC and three hours later were able to detect luciferase enzyme activity, concentrated mainly in the digestive ceca. Subsequently, we tested the presence of an active RNAi pathway in F. hepatica by knocking down the exogenous luciferase activity by introduction into the transformed parasites of double-stranded RNA (dsRNA specific for fLUC. In addition, we tested the RNAi pathway targeting an endogenous F. hepatica gene encoding leucine aminopeptidase (FhLAP, and observed a significant reduction in specific mRNA levels. In summary, these studies demonstrated the utility of RNAi targeting reporter fLUC as a reporter gene assay to establish the presence of an intact RNAi pathway in helminth

  15. The absence of MyD88 has no effect on the induction of alternatively activated macrophage during Fasciola hepatica infection

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    Luo HongLin

    2011-11-01

    Full Text Available Abstract Background Alternatively activated macrophages (AAMϕ play important roles in allergies and responses to parasitic infections. However, whether signaling through toll-like receptors (TLRs plays any role in AAMϕ induction when young Fasciola hepatica penetrates the liver capsule and migrates through the liver tissue is still unclear. Results The data show that the lack of myeloid differentiation factor 88 (MyD88 has no effect on the AAMϕ derived from the bone marrow (BMMϕ in vitro and does not impair the mRNA expression of arginase-1, resistin-like molecule (RELMα, and Ym1 in BMMϕs. The Th2 cytokine production bias in splenocytes was not significantly altered in F. hepatica-infected mice in the absence of MyD88 in vitro and in the pleural cavity lavage in vivo. In addition, MyD88-deficiency has no effect on the arginase production of the F. hepatica elicited macrophages (Fe Mϕs, production of RELMα and Ym1 proteins and mRNA expression of Ym1 and RELMα of macrophages in the peritoneal cavity 6 weeks post F. hepatica infection. Conclusions The absence of MyD88 has no effect on presence of AAMϕ 6 weeks post F. hepatica infection.

  16. Bulk milk-estimated seroprevalence of Fasciola hepatica in dairy herds and collecting of risk factor data in East Frisia, northern Germany.

    Science.gov (United States)

    Kuerpick, Birte; Fiedor, Christiane; von Samson-Himmelstjerna, Georg; Schnieder, Thomas; Strube, Christina

    2012-01-01

    The liver fluke Fasciola (F) hepatica is one of the most important trematodes in cattle farming worldwide. Fasciolosis in dairy cows is leading to production losses due to decreased milk yield, liver condemnation and impaired reproduction. The treatment of dairy cows is unsatisfactory, because available drugs are either effective against adult flukes only or have long withdrawal times or in some countries may not be used at all. In the present study the prevalence of F. hepatica in dairy farms located in East Frisia, which is part of the federal state Lower Saxony, was investigated. East Frisia is considered a high risk area for Fasciola hepatica infections, because of its coastal location, high precipitation and moist pastures. About 750 bulk milk samples were collected in January and November 2006 and analysed for F. hepatica antibodies using the Pourquier ELISA. In addition, questionnaires, which were answered by 260 of the participating farmers, were evaluated to analyse management-related factors associated with fasciolosis. In January and November, 52.1% and 53.6% of the bulk milk samples, respectively, showed positive results. Thereby, 88.1% of the examined farms showed an unchanged infection status, whereas 6.2% of the farms became seropositive during the grazing season and 5.8% of the dairy herds turned seronegative. Statistical analysis revealed a significant negative association between average annual milk production and the frequency of infections with F. hepatica.

  17. Antibody response in cattle, sheep and rats to infection with. gamma. -irradiated metacercariae of Fasciola hepatica

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    Hughes, D.L.; Doy, T.G. (Agricultural Research Council, Compton (UK). Inst. for Research on Animal Diseases); Hanna, R.E.B. (Queen' s Univ., Belfast, Northern Ireland (UK))

    1982-05-01

    Cattle, sheep and rats were infected orally with ..gamma..-irradiated metacercariae of Fasciola hepatica, or with normal metacercariae. The antibody response was monitored in each host to metacercarial tegument (T0), juvenile tegument (T1), adult tegument (T2) and gut antigens. The response was examined at weekly intervals for cattle and sheep throughout 15 weeks of infection and four weeks after infection in rats, using an indirect fluorescent antibody labelling technique. It was found that the irradiated metacercariae engendered a normal humoral response to T0, T1 and gut antigens in all three hosts although the antibody levels were somewhat reduced due to early death or stunting of the flukes. T0 and T1 appeared to be antigenically similar. Antibodies against T2 appeared late in the animals infected with ..gamma..-irradiated metacercariae and the titres attained were considerably lower than in the controls. The T2 antigen stimulus in the animals given ..gamma..-irradiated metacercariae was probably provided by flukes which 'broke through' the developmental barrier imposed by irradiation and which were found alive at autopsy.

  18. Rearing of Lymnaea columella (Say, 1817, intermediate host of Fasciola hepatica (Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Souza Cecília Pereira de

    2000-01-01

    Full Text Available The intermediate host of Fasciola hepatica, Lymnaea columella, collected in Belo Horizonte, Minas Gerais, Brazil, was reared in our laboratory. The aim of the current study was to standardize a rearing and maintenance technique. Two kinds of diet were tested: fresh lettuce (A and rodent ration + 10% CaCO3 plus fresh lettuce (B. The age for the beginning of oviposition ranged from 27 to 57 days. Ten days after oviposition at 24.7°C, 100% eclosion occurred. The complete life cycle varied from 37 to 67 days. The average numbers of eggs per egg mass were 26.3 and 31.1 with diets (A and (B, respectively. The lettuce and ration fed snails presented a increased growth although the difference was not statistically significant (p > 0.05. The mortality rate varied from 40 to 64% after 90 days. The maximum longevity was 183 days, 21.5 mm length and 11 mm wide. The methodology to mass breed and maintain these snails was found to be suitable in the laboratory

  19. Ecology and morphology of the flowers of Hepatica nobilis Schreb. (Ranunculaceae

    Directory of Open Access Journals (Sweden)

    Beata Żuraw

    2013-04-01

    Full Text Available The study was conducted in the years 2009-2010 in the UMCS Botanical Garden in Lublin. The observations involved the flowering time and the seasonal and diurnal dynamics of flowering and pollen production in flowers of the liverleaf (Hepatica nobilis Schreb. from the family Ranunculaceae. The liverleaf begins blooming in early March and the full bloom occurs at the end of March and beginning of April. All-day observations demonstrated that the flowers opened between 8 am and 3 pm, and pollen was released from the anthers between 10 am and 4 pm. The mean weight of pollen produced by 10 liverleaf flowers was estimated at 7.4 mg (1.85 mg/100 stamens. An impact of the weather on the flowering period as well as the seasonal and diurnal dynamics of flowering was observed. Among insect pollinators, the honeybee dominated with a density of 4 individuals per 1m² of a flowerbed that contained 74 open flowers on average.

  20. Whole-body magnetic resonance imaging for staging and follow-up of pediatric patients with Hodgkin's lymphoma: comparison of different sequences; Aplicacao da ressonancia magnetica de corpo inteiro para o estadiamento e acompanhamento de pacientes com linfoma de Hodgkin na faixa etaria infanto-juvenil: comparacao entre diferentes sequencias

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    Nava, Daniel; Oliveira, Heverton Cesar de, E-mail: daniel@centrus.com.b [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem; Luisi, Flavio Augusto; Lederman, Henrique Manoel [Universidade Federal de Sao Paulo (UNIFESP/IOP/GRAACC), SP (Brazil). Inst. de Oncologia Pediatrica. Grupo de Apoio ao Adolescente e a Crianca com Cancer; Ximenes, Andrea Regina da Silveira [Clinica Centrus, Campinas, SP (Brazil)

    2011-07-01

    Objective: to compare the performance of the T1, T2, STIR and DWIBS (diffusion-weighted whole-body imaging with background body signal suppression) sequences in the staging and follow-up of pediatric patients with Hodgkin's lymphoma in lymph node chains, parenchymal organs and bone marrow, and to evaluate interobserver agreement. Materials and methods: the authors studied 12 patients with confirmed diagnosis of Hodgkin's lymphoma. The patients were referred for whole body magnetic resonance imaging with T1-weighted, T2-weighted, STIR and DWIBS sequences. Results: the number of lymph node sites characterized as affected by the disease on T1- and T2-weighted sequences showed similar results (8 sites for both sequences), but lower than DWIBS and STIR sequences (11 and 12 sites, respectively). The bone marrow involvement by lymphoma showed the same values for the T1-, T2-weighted and DWIBS sequences (17 lesions), higher than the value found on STIR (13 lesions). A high rate of interobserver agreement was observed as the four sequences were analyzed. Conclusion: STIR and DWIBS sequences detected the highest number of lymph node sites characterized as affected by the disease. Similar results were demonstrated by all the sequences in the evaluation of parenchymal organs and bone marrow. A high interobserver agreement was observed as the four sequences were analyzed. (author)

  1. Usefulness of magnetic resonance cholangiography in the diagnosis of biliary tract lesions in patients with suspected complication following cholecystectomy; Valor da colangiopancreatografia por ressonancia magnetica no diagnostico de lesoes das vias biliares em pacientes com suspeita de complicacao pos-colecistectomia

    Energy Technology Data Exchange (ETDEWEB)

    Cecin, Alexandre de Oliveira [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil); Goldman, Suzan Menasce; Caetano, Simone; Rosas, George de Queiroz; Coelho, Rafael Darahem de Souza [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem; Lobo, Edson Jose [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Gastroenterologia Cirurgica; Abdalla, Nitamar; Szejnfeld, Jacob [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem

    2005-01-15

    Objective: to determine the usefulness of magnetic resonance cholangiopancreatography (MRCP) in the diagnoses of bile duct injuries following cholecystectomy. Material and method: MRCP was retrospectively evaluated in 40 patients with suspected bile duct injury after laparoscopic or conventional cholecystectomy. Eight of these patients had been submitted to biliary reconstruction. All patients were symptomatic (jaundice, fever and chills, weight loss and abdominal pain). The scans were independently reviewed by two radiologists. The results were confirmed by surgery, percutaneous drainage, endoscopic retrograde cholangiopancreatography and clinical follow-up. The level and severity of bile duct injury were rated according to the Bismuth classification. Results: in a total of 40 symptomatic patients, 10 (25%) had normal findings on MRCP; Postoperative complications were seen in 29 (72.5%) patients, seven of them with more than one finding. Pancreatic head neoplasia was diagnoses in one patient. The most frequent finding was sclerosing (41.4%) followed by biliary duct stenosis (34.5%), residual or recurrent biliary stones (31.0%) and fluid collections (17.2%). The images obtained by MRCP were considered of good quality. Conclusion: MRCP is an effective method for the evaluation of patients with suspected postcholecystectomy biliary tract complications. (author)

  2. Homogeneity characterization of ethylene-co-vinyl acetate copolymer (EVA) and hydrophobic silica nanocomposite by low field NMR; Caracterizacao da homogeneidade de nanocomposito do copolimero etileno acetato de vinila (EVA) e silica hidrofobica atraves de ressonancia magnetica nuclear de baixo campo

    Energy Technology Data Exchange (ETDEWEB)

    Stael, Giovanni Chaves [Observatorio Nacional, Rio de Janeiro, RJ (Brazil). Dept. de Geofisica (DGE); Tavares, Maria I.B. [Universidade Federal do Rio de Janeiro, RJ (Brazil). Inst. de Macromoleculas

    2005-07-01

    This project proposes the characterization of a polymeric matrix composite material using nanometric scale hydrophobic silica as charge element, with the ethylene-vinyl acetate (EVA), by using the spin-lattice relaxation time measurement applying the low field NMR.

  3. Algorithm for three dimension reconstruction of magnetic resonance tomographs and X-ray images based on Fast Fourier Transform; Algoritmo para reconstrucao tridimensional de imagens de tomografos de ressonancia magnetica e de raio-X baseado no uso de Transformada Rapida de Fourier

    Energy Technology Data Exchange (ETDEWEB)

    Bueno, Josiane M.; Traina, Agma Juci M. [Sao Paulo Univ., Sao Carlos, SP (Brazil). Inst. de Ciencias Matematicas; Cruvinel, Paulo E. [EMBRAPA, Sao Carlos, SP (Brazil). CNPDIA

    1995-12-31

    This work presents an algorithm for three-dimensional digital image reconstruction. Such algorithms based on the combination of both a Fast Fourier Transform method with Hamming Window and the use of a tri-linear interpolation function. The algorithm allows not only the generation of three-dimensional spatial spin distribution maps for Magnetic Resonance Tomography data but also X and Y-rays linear attenuation coefficient maps for CT scanners. Results demonstrates the usefulness of the algorithm in three-dimensional image reconstruction by doing first two-dimensional reconstruction and rather after interpolation. The algorithm was developed in C++ language, and there are two available versions: one under the DOS environment, and the other under the UNIX/Sun environment. (author) 10 refs., 5 figs.

  4. Uncommon evolution of probable central nervous system histoplasmosis: from leptomeningitis to posterior fossa granuloma. A case report with magnetic resonance images; Evolucao incomum de provavel histoplasmose de sistema nervoso central: de leptomeningite para granuloma da fossa posterior. Relato de caso com imagens por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Carrilho, Paulo Eduardo Mestrinelli; Alves, Orival [Universidade Estadual do Oeste do Parana - UNIOESTE, Cascavel, PR (Brazil). Curso de Medicina. Disciplina de Neurologia e Neurocirurgia]. E-mail: carrilho@certto.com.br; Budant, Manfredo [UNITOM - Unidade de Diagnostico por Imagem, Cascavel, PR (Brazil). Centro de Tomografia; Bozo, Marlon K.; Meirelles, Noel [Universidade Estadual do Oeste do Parana - UNIOESTE, Cascavel, PR (Brazil). Curso de Medicina; Bueno, Alexandre Galvao [ANATOM - Instituto de Anatomia Patologica de Cascavel, PR (Brazil)

    2006-01-15

    We report a case of a young immunocompetent patient with probable central nervous system histoplasmosis with evolutive peculiar findings seen on magnetic resonance imaging. Leptomeningeal thickening was initially observed which subsequently became a posterior fossa granuloma. The diagnosis of fungal infection was only reached by histopathological study and the treatment was based on long term therapy with fluconazole wth good initial response. (author)

  5. The possibility of recurrent selection to increase the oil content in soybeans using genetic male sterility and NMR spectroscopy; Possibilidade de selecao recorrente para aumento do teor de oleo em soja com a utilizacao da macho-esterilidade genetica e da espectroscopia de ressonancia nuclear magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, M A.C. de; Teixeira, J P.F.; Mascarenhas, H A.A. [Instituto Agronomico de Campinas, SP (Brazil); Rettori, C [Universidade Estadual de Campinas, SP (Brazil). Inst. de Fisica

    1990-12-31

    The seed oil content of male sterile plants and their progenies was analysed by a NMR spectroscope to study the possibility of recurrent selection to improve the oil content. The results suggest the possibility of recurrent selection in the composite IAC-1 both in the field with male sterile plants or at the greenhouse when selection is based on average values of progenies. 30 refs., 3 figs., 3 tabs.

  6. Study using {sup 1} H and {sup 13} V NMR of 3-aryl-s-triazole benzoate azole type compounds and intermediaries; Estudo por ressonancia magnetica nuclear de {sup 1} H e {sup 13} C compostos do tipo 3-aril-s-triazolo benzotiazol e seus intermediarios

    Energy Technology Data Exchange (ETDEWEB)

    Garske, Jose Emilio L; Bacha, Catarina T.M.; Schenkel, Eloir Paulo [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Faculdade de Farmacia

    1992-12-31

    Approximately 62% of the compounds used for medical purposes are heterocyclic, and are distributed as follows: 95% containing hydrogen, 28% containing sulfur and 18% containing oxygen in the structural composition. Some triazole-s-triazole type hetero aromatic systems and intermediaries, such as 1-aryl hydrazides exhibited bactericide, anti inflammatory and fungi stat activities. All the triazoles are are obtained synthetically, and are not found in the Nature. The proton and carbon-13 spectra of the non usual I, II and III compounds that we obtained are discussed in this work 8 refs., 3 figs.

  7. Predictive values of Bi-Rads categories 3, 4 and 5 in non-palpable breast masses evaluated by mammography, ultrasound and magnetic resonance imaging; Valores preditivos das categorias 3, 4 e 5 do sistema Bi-Rads em lesoes mamarias nodulares nao-palpaveis avaliadas por mamografia, ultra-sonografia e ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Roveda Junior, Decio; Fleury, Eduardo de Castro Faria [Santa Casa de Misericordia de Sao Paulo, SP (Brazil). Faculdade de Ciencias Medicas. Servico de Diagnostico por Imagem]. E-mail: decio.jr@uol.com.br; Piato, Sebastiao [Santa Casa de Misericordia de Sao Paulo, SP (Brazil). Dept. de Obstetricia e Ginecologia. Clinica Ginecologica; Oliveira, Vilmar Marques de [Santa Casa de Misericordia de Sao Paulo, SP (Brazil). Dept. de Obstetricia e Ginecologia. Ginecologia Geral; Rinaldi, Jose Francisco [Santa Casa de Misericordia de Sao Paulo, SP (Brazil). Dept. de Obstetricia e Ginecologia. Clinica de Mastologia; Ferreira, Carlos Alberto Pecci [Santa Casa de Misericordia de Sao Paulo, SP (Brazil). Faculdade de Ciencias Medicas. Dept. de Clinica Medica. Servico de Imagenologia Mamaria

    2007-03-15

    Objective: To evaluate the predictive value of BI-RADS{sup TM} categories 3, 4 and 5 in non-palpable breast masses assessed by mammography, ultrasound and magnetic resonance imaging. Materials And Methods: Twenty-nine patients with BI-RADS categories 3, 4 and 5 non-palpable breast masses identified by mammograms were submitted to complementary ultrasound and magnetic resonance imaging studies, besides excisional biopsy. In total, 30 biopsies were performed. The lesions as well as their respective BI-RADS classification into 3, 4 and 5 were correlated with the histopathological results. The predictive values calculation was made by means of specific mathematical equations. Results: Negative predictive values for category 3 were: mammography, 69.23%; ultrasound, 70.58%; and magnetic resonance imaging, 100%. Positive predictive values for category 4 were: mammography, 63.63%; ultrasound, 50%; and magnetic resonance imaging, 30.76%. For category 5, positive predictive values were: mammography and ultrasound, 100%; and magnetic resonance imaging, 92.85%. Conclusion: For category 3, the negative predictive value of magnetic resonance imaging was high, and for categories 4 and 5, the positive predictive values of the three modalities were moderate. (author)

  8. Studies concerning the interaction between local anesthetics and lipid membrane by phosphorus-31, deuterium and proton NMR; Estudo da interacao entre anestesicos locais e membranas lipidicas por ressonancia magnetica de fosforo ({sup 31} P), deuterio ({sup 2} H) e proton ({sup 1} H)

    Energy Technology Data Exchange (ETDEWEB)

    Paula, Eneida de [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Dept. de Bioquimica; Jarrell, Harold C. [National Research Council of Canada, Ottawa, ON (Canada). Inst. for Biological Sciences; Schreier, Shirley [Sao Paulo Univ., SP (Brazil). Inst. de Quimica

    1993-12-31

    Local anesthetics block the conduction of nervous stimulus by impeding the entrance of sodium ion and the consequently depolarization of the nervous membrane. The action mechanism of local anesthetics, however, is not fully understood yet. In the present work the interaction between local anesthetics and membranes are studied by the perspective of lipid phase perturbation using NMR to elucidate the mechanism. Results are presented and discussed 5 refs., 1 fig., 1 tab.

  9. Local anesthetics: interaction with human erythrocyte membranes as studied by {sup 1}H and {sup 31}P nuclear magnetic resonance; Anestesicos locais: interacao com membranas de eritrocitos de sangue humano, estudada por ressonancia magnetica nuclear de {sup 1}H e {sup 31}P

    Energy Technology Data Exchange (ETDEWEB)

    Fraceto, Leonardo Fernandes; Paula, Eneida de [Universidade Estadual de Campinas, SP (Brazil). Inst. de Biologia. Dept. de Bioquimica]. E-mail: depaula@unicamp.br

    2004-02-01

    The literature carries many theories about the mechanism of action of local anesthetics (LA). We can highlight those focusing the direct effect of LA on the sodium channel protein and the ones that consider the interaction of anesthetic molecules with the lipid membrane phase. The interaction between local anesthetics and human erythrocyte membranes has been studied by {sup 1}H and {sup 31}P nuclear magnetic resonance spectroscopy. It was found that lidocaine (LDC) and benzocaine (BZC) bind to the membranes, increase the mobility of the protons of the phospholipids acyl chains, and decrease the mobility and/or change the structure of the polar head groups. The results indicate that lidocaine molecules are inserted across the polar and liquid interface of the membrane, establishing both electrostatic (charged form) and hydrophobic (neutral form) interactions. Benzocaine locates itself a little deeper in the bilayer, between the interfacial glycerol region and the hydrophobic core. These changes in mobility or conformation of membrane lipids could affect the Na{sup +}-channel protein insertion in the bilayer, stabilizing it in the inactivated state, thus causing anesthesia. (author)

  10. Comparative study of fast T 2-weighted images using respiratory triggered, breath-hold, fat suppression and phased array multi coil for liver evaluation by magnetic resonance imaging; Estudo comparativo das sequencias rapidas ponderadas em T2, utilizando-se sincronizacao respiratoria, apneia, supressao de gordura, bobina de corpo e bobina de sinergia para a avaliacao do figado pela ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Abbehusen, Cristiane L.; D' Ippolito, Giuseppe; Palacio, Glaucia A.S.; Szejnfeld, Jacob [Universidade Federal de Sao Paulo (UNIFESP), SP (Brazil). Escola Paulista de Medicina (EPM). Dept. de Diagnostico por Imagem]. E-mail: cabbehusen@hotmail.com

    2003-10-01

    The objective of this study was to compare both qualitatively and quantitatively six T 2-weighted turbo spin-echo sequences varying the respiratory compensation technique, associating or not fat tissue suppression and using different types of coils. We performed a prospective study of 71 consecutive patients that were submitted to MRI of the liver using a 1.5 T magnet. The six following pulse sequences were used: fat-suppressed respiratory triggered with conventional body coil; breath-hold fat-suppressed with conventional body coil; non-suppressed respiratory triggered with conventional body coil; breath-hold non fat-suppressed with conventional body coil; fat-suppressed respiratory triggered with phased-array multi coil; breath-hold fat-suppressed with phased-array multi coil. Images were analyzed quantitatively by measuring the signal-to-noise ratios and qualitatively by evaluating the sharpness of hepatic contours, visibility of intrahepatic vessels and other segmental landmarks, and the presence of artifacts. Results: the qualitative analysis showed that the mean values obtained with the six sequences were 7.8, 4.6, 7.9, 5.2, 6.7 and 4.6 respectively. The respiratory-triggered sequences were better than the breath-hold sequences in both qualitative and quantitative analysis (p < 0.001). No significant differences in the values of signal-to-noise ratios and in overall image quality were found between the sequences with and without fat suppression (p . 0.05). The sequences using the body coil were similar in terms of image quality (p . 0.05) and better regarding signal-to-noise ratios than those obtained with the phased=array multi coil (p ,0.001). Our qualitative and quantitative results suggest that the best MRI sequences for the valuation of the liver are the sequences with respiratory triggering using a conventional body coil, with or without fat suppression. (author)

  11. Evaluation of agreement between transvaginal ultrasonography and magnetic resonance imaging of the pelvis in deep endometriosis with emphasis on intestinal involvement; Avaliacao da concordancia entre a ultrassonografia transvaginal e a ressonancia magnetica da pelve na endometriose profunda, com enfase para o comprometimento intestinal

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, Maene Marcondes; Coutinho Junior, Antonio Carlos; Domingues, Marisa Nassar Aidar [Clinica de Diagnostico por Imagem (CDPI), Rio de Janeiro, RJ (Brazil)], e-mail: maenemc@oi.com.br; Werner Junior, Heron [Clinica de Diagnostico por Imagem (CDPI), Rio de Janeiro, RJ (Brazil). Unit of Gynecologic/Obstetric Radiology; Berardo, Plinio Tostes [Universidade Estacio de Sa, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina; Gasparetto, Emerson Leandro [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia; Domingues, Romeu Cortes [Clinicas Multi-Imagem, Rio de Janeiro, RJ (Brazil)

    2009-03-15

    Objective: To compare sonographic and magnetic resonance imaging findings in deep endometriosis with emphasis on intestinal involvement. Materials and methods: Eighteen women aged between 23 and 49 years with clinical suspicion and gynecological signs suggestive of deep endometriosis were submitted to ultrasonography and magnetic resonance imaging for correlation between findings. Results: Ultrasonography detected 40 lesions while magnetic resonance imaging detected 53 lesions in the pelvis. A comparative study has not shown any statistically significant intermethod difference in the detection of lesions (respectively p > 0.19 and p > 0.14). In the rectosigmoid junction, magnetic resonance imaging has detected one (5.6%) lesion, while ultrasonography has detected four lesions (22.2%). In the rectum, ultrasonography has detected eight lesions (44.4%), and magnetic resonance imaging has detected seven lesions (38.9%). Conclusion: The intermethod agreement has not been good for lesions in the rectosigmoid junction, considering that ultrasonography has detected a higher number of lesions in this region, but a lower number of lesions in the pelvis as compared with magnetic resonance imaging. The global comparative analysis has demonstrated no statistically significant intermethod difference in the detection of lesions. Low cost, good tolerability and high availability make ultrasonography a valuable diagnostic tool in cases of deep endometriosis. (author)

  12. Fasciola hepatica infection and association with gastrointestinal parasites in Creole goats from western Argentina Fasciola hepatica infecção e associação com parasitas gastrintestinais em caprinos crioulos do oeste da Argentina

    Directory of Open Access Journals (Sweden)

    Pablo Cuervo

    2013-08-01

    Full Text Available Goats, called “the cow of the poor”, are the livestock species with the most significant population growth worldwide in recent years. Gastrointestinal parasitism constitutes one of the main constraints to its outdoor and extensive breeding in temperate and tropical countries. Despite a Creole goat population of nearly 4 million heads, local reports on parasitological prevalence are scarce, and while Fasciola hepatica infection is spread all over Argentina, the goat is usually neglected as a reservoir and economic losses are not considered. To evaluate gastrointestinal parasitism prevalence and associations between parasite genera and species, with emphasis on fascioliasis, Creole goats from the plateau and Andean regions from western Argentina were investigated by coprological techniques, and associations were statistically assessed. Eighty-five percent (85% of the animals harbored one or more parasite types, while 46% showed mixed infections. Significant positive associations between F. hepatica + Strongyle eggs, Eimeria sp. + Nematodirus sp. and Nematodirus sp. + Trichuris ovis were detected. Further studies are required to define the causality of these associations and their relevance in epidemiology. F. hepatica is rarely considered as goat parasite in the country, but a 33% prevalence poses an interrogation on the role goats play on the transmission and dissemination of this zoonotic trematode.As cabras, nomeadas como “a vaca dos pobres”, são as espécies de gado com o crescimento populacional mais significativo nos últimos anos em todo o mundo. O parasitismo gastrintestinal constitui uma das principais limitações à sua criação extensiva em clima temperado e tropical. Na Argentina, apesar de uma população de caprinos crioulos de cerca de quatro milhões de cabeças, são escassos os relatórios locais de prevalências parasitológicas. Embora a infecção por Fasciola hepatica esteja espalhada em todo o país, as cabras s

  13. Coproantígenos de Fasciola hepatica de posible utilidad en el diagnóstico de la fascioliasis Identification and isolation of coproantigens of Fasciola hepatica that are potentially useful in the diagnosis of fascioliasis

    Directory of Open Access Journals (Sweden)

    Ana M. Espino

    2000-04-01

    Full Text Available En el presente estudio se describen cuáles son los antígenos de Fasciola hepatica que están presentes en las heces de pacientes con fascioliasis crónica y de ratas infectadas experimentalmente con metacercarias de F. hepatica. Mediante el empleo de la técnica de inmunoelectrotransferencia (Western blot con un suero hiperinmune obtenido frente a antígenos de excreción/secreción de adultos de F. hepatica, se pudo demostrar que en las heces de los pacientes se encuentran antígenos de posible interés para el diagnóstico con masas moleculares de 14, 19, 20, 23, 25, 32, 46, 51 y 62 kilodaltons (kDa. Además, se comprobó que algunos de estos péptidos (los de 14, 20, 23 y 51 kDa son reconocidos también por la mayoría de los sueros de pacientes crónicos. Después de purificar por cromatografía de afinidad los antígenos presentes en heces de ratas con 6 a 12 semanas de infección, empleando para ello el anticuerpo monoclonal ES78 acoplado a Sepharosa 4B-CNBr, se identificaron seis polipéptidos de 11, 14, 26, 32, 47 y 51 kDa; otros tres polipéptidos, de 17, 24 y 66 kDa, solo se pudieron identificar en heces de ratas con 10 a 12 semanas de infección. Nuestros resultados sugieren que estos polipéptidos pueden ser antígenos comunes a ambos estadios parasitarios y constituir importantes marcadores de la fascioliasis aguda y crónica, en particular los de 14, 24, 26 y 51 kDa, puesto que reaccionaron con el inmunosuero, los sueros humanos y el anticuerpo monoclonal ES78.This study describes which antigens of Fasciola hepatica are present in the feces of patients with chronic fascioliasis and in the feces of rats infected experimentally with F. hepatica metacercariae. Using a Western blot assay technique with hyperimmune serum obtained from excretory-secretory antigens of adult F. hepatica, we found in the patients' feces antigens of possible diagnostic interest, with molecular weights of 14, 19, 20, 23, 25, 32, 46, 51, and 62 kilodaltons

  14. High-resolution magneto stratigraphic of the Eocene-Oligocene boundary in the Umbria-Marche sequence; Stratigrafia magnetica ad alta risoluzione del limite Eocene-Oligocene nella successione Umbro-Marchigiana

    Energy Technology Data Exchange (ETDEWEB)

    Lanci, L.; Lowrie, W.; Montanari, A.

    1998-12-31

    High-resolution magneto stratigraphy across the Eocene-Olicene boundary has been employed in a detailed investigation of the nature of low-amplitude, short-wavelength oceanic magnetic anomalies. [Italiano] La stratigrafia magnetica ad alta risoluzione del limite Eocene-Oligocene ed il magnetismo delle rocce sono stati studiati in dettaglio per verificare la presenza di eventuali inversioni di polarita` brevi (short chrons) e variazioni delle intensita` del campo geomagnetico che possano essere messe in relazione con le anomalie oceaniche a bassa ampiezza e corto periodo.

  15. Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.

    Science.gov (United States)

    McNulty, Samantha N; Tort, Jose F; Rinaldi, Gabriel; Fischer, Kerstin; Rosa, Bruce A; Smircich, Pablo; Fontenla, Santiago; Choi, Young-Jun; Tyagi, Rahul; Hallsworth-Pepin, Kymberlie; Mann, Victoria H; Kammili, Lakshmi; Latham, Patricia S; Dell'Oca, Nicolas; Dominguez, Fernanda; Carmona, Carlos; Fischer, Peter U; Brindley, Paul J; Mitreva, Makedonka

    2017-01-01

    Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered trans-kingdom interaction with nFh and the impact of both species on disease in ruminants and humans.

  16. Fasciola hepatica demonstrates high levels of genetic diversity, a lack of population structure and high gene flow: possible implications for drug resistance.

    Science.gov (United States)

    Beesley, Nicola J; Williams, Diana J L; Paterson, Steve; Hodgkinson, Jane

    2017-01-01

    Fasciola hepatica, the liver fluke, is a trematode parasite of considerable economic importance to the livestock industry and is a re-emerging zoonosis that poses a risk to human health in F. hepatica-endemic areas worldwide. Drug resistance is a substantial threat to the current and future control of F. hepatica, yet little is known about how the biology of the parasite influences the development and spread of resistance. Given that F. hepatica can self-fertilise and therefore inbreed, there is the potential for greater population differentiation and an increased likelihood of recessive alleles, such as drug resistance genes, coming together. This could be compounded by clonal expansion within the snail intermediate host and aggregation of parasites of the same genotype on pasture. Alternatively, widespread movement of animals that typically occurs in the UK could promote high levels of gene flow and prevent population differentiation. We identified clonal parasites with identical multilocus genotypes in 61% of hosts. Despite this, 84% of 1579 adult parasites had unique multilocus genotypes, which supports high levels of genotypic diversity within F. hepatica populations. Our analyses indicate a selfing rate no greater than 2%, suggesting that this diversity is in part due to the propensity for F. hepatica to cross-fertilise. Finally, although we identified high genetic diversity within a given host, there was little evidence for differentiation between populations from different hosts, indicating a single panmictic population. This implies that, once those emerge, anthelmintic resistance genes have the potential to spread rapidly through liver fluke populations. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  17. Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers

    Science.gov (United States)

    McNulty, Samantha N.; Rosa, Bruce A.; Fontenla, Santiago; Choi, Young-Jun; Hallsworth-Pepin, Kymberlie; Kammili, Lakshmi; Latham, Patricia S.; Dell’Oca, Nicolas; Dominguez, Fernanda; Carmona, Carlos; Fischer, Peter U.; Mitreva, Makedonka

    2017-01-01

    Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis’ gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered trans-kingdom interaction with nFh and the impact of both species on disease in ruminants and humans. PMID:28060841

  18. Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.

    Directory of Open Access Journals (Sweden)

    Samantha N McNulty

    2017-01-01

    Full Text Available Food borne trematodes (FBTs are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs. Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered trans-kingdom interaction with nFh and the impact of both species on disease in ruminants and humans.

  19. A novel ex vivo immunoproteomic approach characterising Fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep.

    Science.gov (United States)

    Cameron, Timothy C; Cooke, Ira; Faou, Pierre; Toet, Hayley; Piedrafita, David; Young, Neil; Rathinasamy, Vignesh; Beddoe, Travis; Anderson, Glenn; Dempster, Robert; Spithill, Terry W

    2017-08-01

    A more thorough understanding of the immunological interactions between Fasciola spp. and their hosts is required if we are to develop new immunotherapies to control fasciolosis. Deeper knowledge of the antigens that are the target of the acquired immune responses of definitive hosts against both Fasciola hepatica and Fasciola gigantica will potentially identify candidate vaccine antigens. Indonesian Thin Tail sheep express a high level of acquired immunity to infection by F. gigantica within 4weeks of infection and antibodies in Indonesian Thin Tail sera can promote antibody-dependent cell-mediated cytotoxicity against the surface tegument of juvenile F. gigantica in vitro. Given the high protein sequence similarity between F. hepatica and F. gigantica, we hypothesised that antibody from F. gigantica-infected sheep could be used to identify the orthologous proteins in the tegument of F. hepatica. Purified IgG from the sera of F. gigantica-infected Indonesian Thin Tail sheep collected pre-infection and 4weeks p.i. were incubated with live adult F. hepatica ex vivo and the immunosloughate (immunoprecipitate) formed was isolated and analysed via liquid chromatography-electrospray ionisation-tandem mass spectrometry to identify proteins involved in the immune response. A total of 38 proteins were identified at a significantly higher abundance in the immunosloughate using week 4 IgG, including eight predicted membrane proteins, 20 secreted proteins, nine proteins predicted to be associated with either the lysosomes, the cytoplasm or the cytoskeleton and one protein with an unknown cellular localization. Three of the membrane proteins are transporters including a multidrug resistance protein, an amino acid permease and a glucose transporter. Interestingly, a total of 21 of the 38 proteins matched with proteins recently reported to be associated with the proposed small exosome-like extracellular vesicles of adult F. hepatica, suggesting that the Indonesian Thin Tail week

  20. Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F. hepatica

    Science.gov (United States)

    2014-01-01

    Background Fascioliasis is an important and neglected disease of humans and other mammals, caused by trematodes of the genus Fasciola. Fasciola hepatica and F. gigantica are valid species that infect humans and animals, but the specific status of Fasciola sp. (‘intermediate form’) is unclear. Methods Single specimens inferred to represent Fasciola sp. (‘intermediate form’; Heilongjiang) and F. gigantica (Guangxi) from China were genetically identified and characterized using PCR-based sequencing of the first and second internal transcribed spacer regions of nuclear ribosomal DNA. The complete mitochondrial (mt) genomes of these representative specimens were then sequenced. The relationships of these specimens with selected members of the Trematoda were assessed by phylogenetic analysis of concatenated amino acid sequence datasets by Bayesian inference (BI). Results The complete mt genomes of representatives of Fasciola sp. and F. gigantica were 14,453 bp and 14,478 bp in size, respectively. Both mt genomes contain 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes, but lack an atp8 gene. All protein-coding genes are transcribed in the same direction, and the gene order in both mt genomes is the same as that published for F. hepatica. Phylogenetic analysis of the concatenated amino acid sequence data for all 12 protein-coding genes showed that the specimen of Fasciola sp. was more closely related to F. gigantica than to F. hepatica. Conclusions The mt genomes characterized here provide a rich source of markers, which can be used in combination with nuclear markers and imaging techniques, for future comparative studies of the biology of Fasciola sp. from China and other countries. PMID:24685294

  1. Complete mitochondrial genomes of the 'intermediate form' of Fasciola and Fasciola gigantica, and their comparison with F. hepatica.

    Science.gov (United States)

    Liu, Guo-Hua; Gasser, Robin B; Young, Neil D; Song, Hui-Qun; Ai, Lin; Zhu, Xing-Quan

    2014-03-31

    Fascioliasis is an important and neglected disease of humans and other mammals, caused by trematodes of the genus Fasciola. Fasciola hepatica and F. gigantica are valid species that infect humans and animals, but the specific status of Fasciola sp. ('intermediate form') is unclear. Single specimens inferred to represent Fasciola sp. ('intermediate form'; Heilongjiang) and F. gigantica (Guangxi) from China were genetically identified and characterized using PCR-based sequencing of the first and second internal transcribed spacer regions of nuclear ribosomal DNA. The complete mitochondrial (mt) genomes of these representative specimens were then sequenced. The relationships of these specimens with selected members of the Trematoda were assessed by phylogenetic analysis of concatenated amino acid sequence datasets by Bayesian inference (BI). The complete mt genomes of representatives of Fasciola sp. and F. gigantica were 14,453 bp and 14,478 bp in size, respectively. Both mt genomes contain 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes, but lack an atp8 gene. All protein-coding genes are transcribed in the same direction, and the gene order in both mt genomes is the same as that published for F. hepatica. Phylogenetic analysis of the concatenated amino acid sequence data for all 12 protein-coding genes showed that the specimen of Fasciola sp. was more closely related to F. gigantica than to F. hepatica. The mt genomes characterized here provide a rich source of markers, which can be used in combination with nuclear markers and imaging techniques, for future comparative studies of the biology of Fasciola sp. from China and other countries.

  2. Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro.

    Science.gov (United States)

    Pereira, Cíntia A J; Oliveira, Laura L S; Coaglio, Aytube L; Santos, Fernanda S O; Cezar, Rodolfo S M; Mendes, Tiago; Oliveira, Fernando L P; Conzensa, Gustavo; Lima, Walter S

    2016-09-15

    Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid-liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50F. hepatica eggs were incubated at 23°C with M. charantia leaf CE in different concentrations. After 12days no larvae were formed in eggs incubated with CE concentrations above 12.5mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01μg/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC-MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Application of a coproantigen ELISA as an indicator of efficacy against multiple life stages of Fasciola hepatica infections in sheep.

    Science.gov (United States)

    George, S D; Vanhoff, K; Baker, K; Lake, L; Rolfe, P F; Seewald, W; Emery, D L

    2017-11-15

    At present diagnosis of true resistance and determination of drug efficacy in Fasciola hepatica infection rely solely on terminal experiments. The coproantigen ELISA (cELISA) has been reported previously as a sensitive and specific tool appropriate to detect treatment failure, and potentially drug resistance. Two studies were conducted to determine whether the cELISA was appropriate for on-farm efficacy and resistance testing in Australian Merino sheep. In Study 1 sheep were infected orally with 50 F. hepatica metacercariae on three occasions, twelve, six and two weeks prior to a single flukicide treatment with triclabendazole, closantel or albendazole. Sheep were sampled weekly for a further seven weeks prior to necropsy. Following effective treatment, no faecal antigen was detected from 1 week. When immature stages (≤6 weeks) survived treatment, coproantigen reappeared from 6 weeks post-treatment. Therefore, cELISA conducted 1-4 weeks after treatment will demonstrate obvious treatment failure against adult F. hepatica, but is not sufficiently sensitive to detect survival of immature fluke until these reach maturity. In study 2, fluke burdens of sheep necropsied 13 weeks post single infection were compared to fecal worm egg counts (FWEC) and cELISA at necropsy. Regression analysis demonstrated that cELISA correlated strongly with fluke burden, whilst FWEC correlated weakly with cELISA. The correlation between FWEC and fluke burden was also weak, although stronger than that of FWEC with cELISA. The cELISA is an appropriate tool for monitoring effectiveness of treatments against Fasciola hepatica if an adult infection is present, however when immature stages of the parasite are present it is not as reliable. Where immature parasites are present it is recommended that initial cELISA be followed with a secondary cELISA at least 6 weeks after treatment to ensure resistance to immature stages is detected. Further testing is justified for monitoring the effectiveness

  4. Apparent prevalence of and risk factors for infection with Ostertagia ostertagi, Fasciola hepatica and Dictyocaulus viviparus in Swiss dairy herds.

    Science.gov (United States)

    Frey, C F; Eicher, R; Raue, K; Strube, C; Bodmer, M; Hentrich, B; Gottstein, B; Marreros, N

    2018-01-30

    Infections with helminth parasites can negatively affect performance of dairy cows. Knowledge on infection intensity, spatial distributions and risk factors are key to develop targeted treatment strategies. Canada and most EU countries have conducted large investigations, but respective data for Switzerland were missing. We now performed a bulk tank milk serosurvey for Ostertagia ostertagi, Fasciola hepatica, and Dictyocaulus viviparus on a total of 1036 voluntarily participating dairy herds that were sampled at confinement periods, i.e. in winter 2014/15 or 2015/16, respectively. All samples were analyzed with commercial ELISAs for antibodies (AB) against O. ostertagi and F. hepatica, and those of the first sampling period additionally with an in-house ELISA for AB against D. viviparus. Testing for the latter parasite was not done in the second year of the study, as the sampling period might have missed infections due to the short lived nature of specific antibodies. The possible influence of geographic, climatic, and farm management variables on AB levels were assessed for each parasite using scanning cluster and multiple regression analysis. Overall seroprevalence for O. ostertagi was 95.5% (95% C.I.: 94.0-96.6), with a mean optical density ratio (ODR) of 0.83, for F. hepatica 41.3% (95% C.I.: 38.3-44.4), and for D. viviparus 2.9% (95% C.I.: 1.6-4.7). There were no significant differences between the two sampling periods. For all parasites, significant geographic clusters of higher AB levels could be established. Furthermore, AB levels against all three parasites were positively correlated with each other, indicating either cross-reactions or co-infections. For O. ostertagi, herd size and percentage of pasture in the ration were positively correlated with AB levels. For F. hepatica, altitude above sea level (a.s.l.) positively, and milk production per cow and year was negatively correlated with AB levels. This work provides baseline data for further

  5. The diterpenoid 7-keto-sempervirol, derived from Lycium chinense, displays anthelmintic activity against both Schistosoma mansoni and Fasciola hepatica.

    Directory of Open Access Journals (Sweden)

    Jennifer Edwards

    2015-03-01

    Full Text Available BACKGROUND: Two platyhelminths of biomedical and commercial significance are Schistosoma mansoni (blood fluke and Fasciola hepatica (liver fluke. These related trematodes are responsible for the chronic neglected tropical diseases schistosomiasis and fascioliasis, respectively. As no vaccine is currently available for anti-flukicidal immunoprophylaxis, current treatment is mediated by mono-chemical chemotherapy in the form of mass drug administration (MDA (praziquantel for schistosomiasis or drenching (triclabendazole for fascioliasis programmes. This overreliance on single chemotherapeutic classes has dramatically limited the number of novel chemical entities entering anthelmintic drug discovery pipelines, raising significant concerns for the future of sustainable blood and liver fluke control. METHODOLOGY/ PRINCIPLE FINDINGS: Here we demonstrate that 7-keto-sempervirol, a diterpenoid isolated from Lycium chinense, has dual anthelmintic activity against related S. mansoni and F. hepatica trematodes. Using a microtiter plate-based helminth fluorescent bioassay (HFB, this activity is specific (Therapeutic index = 4.2, when compared to HepG2 cell lines and moderately potent (LD50 = 19.1 μM against S. mansoni schistosomula cultured in vitro. This anti-schistosomula effect translates into activity against both adult male and female schistosomes cultured in vitro where 7-keto-sempervirol negatively affects motility/behaviour, surface architecture (inducing tegumental holes, tubercle swelling and spine loss/shortening, oviposition rates and egg morphology. As assessed by the HFB and microscopic phenotypic scoring matrices, 7-keto-sempervirol also effectively kills in vitro cultured F. hepatica newly excysted juveniles (NEJs, LD50 = 17.7 μM. Scanning electron microscopy (SEM evaluation of adult F. hepatica liver flukes co-cultured in vitro with 7-keto-sempervirol additionally demonstrates phenotypic abnormalities including breaches in tegumental

  6. Spatial analysis and risk mapping of Fasciola hepatica infection in dairy herds in Ireland

    Directory of Open Access Journals (Sweden)

    Nikolaos Selemetas

    2015-03-01

    Full Text Available Fasciolosis is generally a subclinical infection of dairy cows and can cause marked economic losses. This study investigated the prevalence and spatial distribution of fasciolosis in dairy cow herds in Ireland using an in-house antibodydetection enzyme-linked immunosorbent assay applied to bulk tank milk (BTM samples collected during the autumn of 2012. A total of 5,116 BTM samples were collected from 4,602 different herds, with 514 farmers submitting BTM samples in two consecutive months. Analysis of the BTM samples showed that 82% (n = 3,764 of the dairy herds had been exposed to Fasciola hepatica. A total of 108 variables, including averaged climatic data for the period 1981-2010 and contemporary meteorological data for the year 2012, such as soil, subsoil, land cover and habitat maps, were investigated for a possible role as predictor of fasciolosis. Using mainly climatic variables as the major predictors, a model of the predicted risk of fasciolosis was created by Random Forest modelling that had 95% sensitivity and 100% specificity. The most important predictors in descending order of importance were: average of annual total number of rain-days for the period 1981-2010, total rainfall during September, winter and autumn of 2012, average of annual total number of wet-days for the period 1981- 2010 and annual mean temperature of 2012. The findings of this study confirm the high prevalence of fasciolosis in Irish dairy herds and suggest that specific weather and environmental risk factors support a robust and precise distribution model.

  7. Modelling the spatial distribution of Fasciola hepatica in dairy cattle in Europe

    Directory of Open Access Journals (Sweden)

    Els Ducheyne

    2015-03-01

    Full Text Available A harmonized sampling approach in combination with spatial modelling is required to update current knowledge of fasciolosis in dairy cattle in Europe. Within the scope of the EU project GLOWORM, samples from 3,359 randomly selected farms in 849 municipalities in Belgium, Germany, Ireland, Poland and Sweden were collected and their infection status assessed using an indirect bulk tank milk (BTM enzyme-linked immunosorbent assay (ELISA. Dairy farms were considered exposed when the optical density ratio (ODR exceeded the 0.3 cut-off. Two ensemble-modelling techniques, Random Forests (RF and Boosted Regression Trees (BRT, were used to obtain the spatial distribution of the probability of exposure to Fasciola hepatica using remotely sensed environmental variables (1-km spatial resolution and interpolated values from meteorological stations as predictors. The median ODRs amounted to 0.31, 0.12, 0.54, 0.25 and 0.44 for Belgium, Germany, Ireland, Poland and southern Sweden, respectively. Using the 0.3 threshold, 571 municipalities were categorized as positive and 429 as negative. RF was seen as capable of predicting the spatial distribution of exposure with an area under the receiver operation characteristic (ROC curve (AUC of 0.83 (0.96 for BRT. Both models identified rainfall and temperature as the most important factors for probability of exposure. Areas of high and low exposure were identified by both models, with BRT better at discriminating between low-probability and high-probability exposure; this model may therefore be more useful in practise. Given a harmonized sampling strategy, it should be possible to generate robust spatial models for fasciolosis in dairy cattle in Europe to be used as input for temporal models and for the detection of deviations in baseline probability. Further research is required for model output in areas outside the eco-climatic range investigated.

  8. The parasitic castration and gigantism of Lymnaea truncatula infected with the larval stages of Fasciola hepatica.

    Science.gov (United States)

    Wilson, R A; Denison, J

    1980-01-01

    The shells of Lymnaea truncatula infected with the larval stages of Fasciola hepatica were significantly longer than those of comparable uninfected controls. The dry mass (tissue, shell + parasite) of the same infected snails, 56 days after infection, was approximately twice that of the controls (tissue + shell). The increased mass of infected snails was not due to a disproportionate increase in shell weight relative to tissues. Infected snails maintained at 20 degrees C had virtually ceased egg production by 21 days post-infection whereas control snails continued to lay eggs steadily for the duration of the experiment. The dry mass of snail tissue plus the cumulative dry weight of eggs produced was taken as an indication of the ability of control snails to generate biomass. Similarly the tissue mass plus cumulative egg weight and parasite weight was taken as an indication of the ability of the infected snails to generate biomass. The control and infected snails were not significantly different in this respect indicating that the gigantism of infected snails could be the result of a switch in nutrient supply from reproduction to somatic tissue growth and parasite growth. Castration was brought about 17-21 days after infection as a result of the direct consumption of the ovotestis by a proportion of the redial population. In a separate experiment it was demonstrated that a population of infected snails maintained at 20 degrees C survived as long as a similar group of control snails. The findings with this host-parasite system are discussed in relation to possible mechanisms causing castration and gigantism in other digene-snail interactions, and in relation to parasitic castration in other groups. It is concluded that the observed gigantism of infected snails is more likely to have a nutritional rather than endocrine origin.

  9. Immunoexpression of intermediate filaments and morphological changes in the liver and bile duct of rats infected with Fasciola hepatica.

    Science.gov (United States)

    Kolodziejczyk, L; Laszczyńska, M; Masiuk, M; Grabowska, M; Skrzydlewska, E

    2015-01-01

    We investigated the immunoexpression of the intermediate filament proteins, cytokeratin and desmin, and the morphological changes in the liver of rats during experimental fasciolosis at 4, 7 and 10 weeks post-infection. Rats were infected with 30 Fasciola hepatica metacercariae. Paraffin sections of the liver were stained using H & E, PAS and azan stains. Immunohistochemical reactions were performed using antibodies against cytokeratin and desmin. The experimental F. hepatica infection led to fibrosis and cirrhosis of the liver, and to inflammation of the common bile ducts. The expression of cytokeratin was increased in the epithelial cells of both the liver bile ductules at 4, 7 and 10 weeks post-infection and in the common bile ducts at 7 and 10 weeks post-infection compared to uninfected rats; expression in the common bile ducts was more intense. The myofibroblasts of the liver and smooth myocytes of the interlobular bile ducts and common bile ducts, showed a slight increase in desmin expression compared to the uninfected rats. The increased expression of cytokeratins in the hyperplastic rat common bile duct epithelium during the biliary phase of fasciolosis at 7 and 10 weeks post-infection may be explained by mechanical irritation by the parasite and an inflammatory reaction in the bile duct epithelium and in periductal fibrous tissue.

  10. The Extracellular Vesicles of the Helminth Pathogen, Fasciola hepatica: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis*

    Science.gov (United States)

    Cwiklinski, Krystyna; de la Torre-Escudero, Eduardo; Trelis, Maria; Bernal, Dolores; Dufresne, Philippe J.; Brennan, Gerard P.; O'Neill, Sandra; Tort, Jose; Paterson, Steve; Marcilla, Antonio; Dalton, John P.; Robinson, Mark W.

    2015-01-01

    Extracellular vesicles (EVs) released by parasites have important roles in establishing and maintaining infection. Analysis of the soluble and vesicular secretions of adult Fasciola hepatica has established a definitive characterization of the total secretome of this zoonotic parasite. Fasciola secretes at least two subpopulations of EVs that differ according to size, cargo molecules and site of release from the parasite. The larger EVs are released from the specialized cells that line the parasite gastrodermus and contain the zymogen of the 37 kDa cathepsin L peptidase that performs a digestive function. The smaller exosome-like vesicle population originate from multivesicular bodies within the tegumental syncytium and carry many previously described immunomodulatory molecules that could be delivered into host cells. By integrating our proteomics data with recently available transcriptomic data sets we have detailed the pathways involved with EV biogenesis in F. hepatica and propose that the small exosome biogenesis occurs via ESCRT-dependent MVB formation in the tegumental syncytium before being shed from the apical plasma membrane. Furthermore, we found that the molecular “machinery” required for EV biogenesis is constitutively expressed across the intramammalian development stages of the parasite. By contrast, the cargo molecules packaged within the EVs are developmentally regulated, most likely to facilitate the parasites migration through host tissue and to counteract host immune attack. PMID:26486420

  11. Development and validation of a meat juice ELISA for the diagnosis of Fasciola hepatica in cattle in Cuba

    Directory of Open Access Journals (Sweden)

    Amilcar Arenal

    2016-08-01

    Full Text Available Objective: To establish and validate a home-made ELISA for determination of antibodies against excretory-secretory proteins of Fasciola hepatica in bovine meat juice samples. Methods: The validity criteria of the assay were defined based on standards of ISO. The following parameters were evaluated: excretion/secretion antigen concentrations for coating, anti-bovine immunoglobulin G dilution, linearity, accuracy and precision. Results: The assay was validated on 126 meat juice samples with known infection status. Using the receiver operating characteristic (n = 126 the optimal cut-off for the ELISA assay was 0.78, above this value the probability for an animal to have fasciolosis was 11 times. And the specificity and sensitivity were 100% and 90.91% respectively. The repeatability of the intra- and inter-assay tests had coefficients of variation lower than 10% and 20% respectively. Conclusions: The ELISA is a suitable test for further use in studies towards the epidemiology of Fasciola hepatica in Cuba.

  12. Fascioliasis coledociana por Fasciola hepatica en cirugía de colecistitis crónica calculosa

    Directory of Open Access Journals (Sweden)

    María Beltrán-Fabián

    2011-04-01

    Full Text Available Se reporta un caso de Fasciola hepatica en una mujer de 50 años de edad, natural de Huaral, procedente de un asentamiento humano del distrito de Ate en Lima, con un tiempo de enfermedad de siete días caracterizado al inicio por dolor abdominal tipo cólico de curso progresivo en el epigastrio, dolor que se irradia a hipocondrio derecho. Luego presentó luego náuseas y vómitos en cuatro oportunidades, de contenido alimenticio y bilioso, por lo que se decide su hospitalización previa atención por emergencia. Al examen físico se encontró conjuntivas oculares levemente ictéricas, abdomen balonado, blando, depresible, no visceromegalia, Murphy positivo, ruidos hidroaéreos presentes, tórax y pulmones sin alteraciones. Posteriormente a los exámenes auxiliares, se diagnosticó colecistitis calculosa aguda y fascioliasis coledociana. En el acto quirúrgico se extrajo tres especímenes identificados como Fasciola hepatica. La paciente después de recibir el triclabendazol, concurrió al Hospital para la evaluación y control, habiendo logrado mejoría y bienestar.

  13. A single amino acid substitution in isozyme GST mu in Triclabendazole resistant Fasciola hepatica (Sligo strain) can substantially influence the manifestation of anthelmintic resistance.

    Science.gov (United States)

    Fernández, V; Estein, S; Ortiz, P; Luchessi, P; Solana, V; Solana, H

    2015-12-01

    The helminth parasite Fasciola hepatica causes fascioliasis in human and domestic ruminants. Economic losses due to this infection are estimated in U$S 2000-3000 million yearly. The most common method of control is the use of anthelmintic drugs. However, there is an increased concern about the growing appearance of F. hepatica resistance to Triclabendazole (TCBZ), an anthelmintic with activity over adult and young flukes. F. hepatica has eight Glutathione S-Transferase (GST) isozymes, which are enzymes involved in the detoxification of a wide range of substrates through chemical conjugation with glutathione. In the present work we identified and characterized the GST mu gene isolated from the TCBZ-susceptible and TCBZ-resistant F. hepatica strains. Total RNA was transcribed into cDNA by reverse transcription and a 657 bp amplicon corresponding to the GST mu gene was obtained. The comparative genetic analysis of the GST mu gene of the TCBZ susceptible strain (Cullompton) and TCBZ resistant strain (Sligo) showed three nucleotide changes and one amino acid change at position 143 in the GST mu isozyme of the TCBZ-resistant strain. These results have potential relevance as they contribute better understand the mechanisms that generate resistance to anthelmintics. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Proteomic analysis of Fasciola hepatica excretory and secretory products (FhESPs) involved in interacting with host PBMCs and cytokines by shotgun LC-MS/MS.

    Science.gov (United States)

    Liu, Qing; Huang, Si-Yang; Yue, Dong-Mei; Wang, Jin-Lei; Wang, Yujian; Li, Xiangrui; Zhu, Xing-Quan

    2017-02-01

    Fasciola hepatica is a helminth parasite with a worldwide distribution, which can cause chronic liver disease, fasciolosis, leading to economic losses in the livestock and public health in many countries. Control is mostly reliant on the use of drugs, and as a result, drug resistance has now emerged. The identification of F. hepatica genes involved in interaction between the parasite and host immune system is utmost important to elucidate the evasion mechanisms of the parasite and develop more effective strategies against fasciolosis. In this study, we aimed to identify molecules in F. hepatica excretory and secretory products (FhESPs) interacting with the host peripheral blood mononuclear cells (PBMCs), Th1-like cytokines (IL2 and IFN-γ), and Th17-like cytokines (IL17) by Co-IP combined with tandem mass spectrometry. The results showed that 14, 16, and 9 proteins in FhESPs could bind with IL2, IL17, and IFN-γ, respectively, which indicated that adult F. hepatica may evade the host immune responses through directly interplaying with cytokines. In addition, nine proteins in FhESPs could adhere to PBMCs. Our findings provided potential targets as immuno-regulators, and will be helpful to elucidate the molecular basis of host-parasite interactions and search for new potential proteins as vaccine and drug target candidates.

  15. Role of the apparent diffusion coefficient measurement by diffusion weighted magnetic resonance imaging in the diagnosis of Fasciola hepatica in the liver

    International Nuclear Information System (INIS)

    Onur, M.R.; Cicekci, M.; Kayali, A.; Aygun, C.; Kocakoc, E.

    2011-01-01

    The aim of this study was to investigate the diagnostic role of the apparent diffusion coefficient (ADC) measurement in the diagnosis of focal parenchymal lesions and to understand the discriminating role of the ADC value for differentiating Fasciola lesions from other focal liver lesions. We measured ADC values of parenchymal lesions and liver parenchyma in 18 patients with Fasciola hepatica infestation at b 100, b 600, and b 1000 s/mm 2 gradients. We further measured average ADC values of hepatic metastases (n=21), hepatocellular carcinomas (n=21), cholangiocarcinomas (n=7), hydatid cysts (n=12), and focal nodular hyperplasia (FNH) (n=12) and compared them with average ADC values for Fasciola hepatica. The differences between average ADC values of lesions (2.16±0.36 x 10 -3 mm 2 /s) and parenchyma (1.64±0.2 x 10 -3 mm 2 /s) at three gradients were statistically significant (P<0.05). Mean ADC values of Fasciola hepatica lesions were significantly different from most of the other focal hepatic lesions, except FNH at all gradients and hydatid cyst at only the b 100 gradient. ADC measurement may be a complementary method in the diagnosis of Fasciola hepatica, and it may be used to differentiate these lesions from other focal liver lesions. (author)

  16. Os trigonum tarsi syndrome. Magnetic Resonance features; Sindrome dell'os trigonum tarsi. Ruolo della Risonanza Magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Iovane, A.; Finazzo, M.; Carcione, A.; De Maria, M; Lagalla, R. [Palermo Policlinico Univ. P. Giaccone, Palermo (Italy). Ist. di Radiologia; Midiri, M. [Bari Policlinico Univ., Bari (Italy). Ist. di Radiologia

    2000-02-01

    aspect of the tibia, or hypo mobility to pseudoarthrosis. Forced plantar flexion acquisitions are particularly useful in this condition because can demonstrate the mechanism of injury. [Italian] L'os trigonum tarsi e' un osso accessorio del piede localizzato in sede posterolaterale rispetto al tubercolo esterno dell'astragalo e di solito non da' alcuna sintomatologia. In particolari condizioni, che comportano sollecitazioni o microtraumi ripetuti nel tempo a livello del retropiede puo' essere responsabile di talalgia posteriore realizzando la cosidetta sindrome dell'os trigonum tarsi. Il dolore e' localizzato nella sede preachillea. La diagnosi tuttavia risulta particolarmente complessa i quanto numerose altre forme patologiche si manifestano con la medesima sintomatologia clinica. Con la radiologia tradizionale e' possibile dimostrare la presenza dell'os trigonum e le sue eventuali alterazioni morfo-strutturali. La RM (Risonanza Magnetica) di contro consente non solo di identificare le alterazioni strutturali dell'osso ma anche di riconoscere le lesioni associate dei tessuti molli. Sono stati studiati con RM nove pazienti affetti da sindrome dell'os trigonum tarsi. I pazienti sono stati esaminati nella posizione supina con il piede in posizione indifferente e successivamente bloccato nella flessione plantare. Lo studio e' stato condotto con magnete superconduttivo da 0,5 T impiegando una bobina dedicata per le estremita'. Sono state utilizzate sequenze spin-echo T1, gradient-echo T2* e fast inversion recovery secondo piani di scansione assiali e saggitali. In tutti i pazienti esaminati sono state valutate: sede e morfologia dell'os trigonum; caratteristiche dell'intensita' del segnale delle strutture ossee, cartilaginee e dei tessuti adiacenti; presenza di eventuali lesioni tendinee associate. Non sono state osservate anomalie di sede ne' di forma dell'os trigonum. L

  17. Dobutamine cine magnetic resonance imaging after myocardial infarction; Cine Risonanza Magnetica con dobutamina dopo infarto del miocardio

    Energy Technology Data Exchange (ETDEWEB)

    Giovagnoni, A.; Ligabue, G.; Romagnoli, R. [Modena Univ., Modena (Italy). Scienze Radiologiche, Dipt. di Medicina Interna; Reggio Emilia Univ., Reggio Emilia (Italy). Cattedra di Cardiologia; Rossi, R.; Muia, N.; Modena, M.G. [Modena Univ., Modena (Italy). Dipt. di Scienze Mediche, Oncologiche e Radiologiche; Reggio Emilia Univ. (Italy). Dipt. di Scienze Mediche, Oncologiche e Radiologiche

    1999-12-01

    . [Italian] La cine Risonanza Magnetica (cine RM) con stimolo farmacologico {beta}-adrenergico (stress dobutamina) rappresenta una nuova indagine diagnostica nella valuazione pretrattamento di rivascolarizzazione del paziente con infarto del miocardio. Scopo dello studio e' quello di verificare la validita' diagnostica della cine RM con stimolo farmacologico in confronto con l'ecocardiografia con stimolo farmacologico nella valutazione della vitalita' del miocardio e di proporre un nuovo metodo d'analisi delle immagini di cine RM mediante la sottrazione elettronica d'immagine al fine di ridurre gli elementi soggettivi nella valutazione quantitativa dell'ispessimento parietale. Ventisei pazienti (21 maschi, 5 femmine) con pregresso infarto del miocardio in attesa di intervento di rivascolarizzazione sono stati sottoposti a ecocardiografia con stimolo farmacologico mediante dobutamina e a esame cine RM con dobutamina al fine di verificare la ripresa contrattile dei segmenti giudicati acinetici o ipocinetici all'esame ecocardiografico di base. E' stata infusa dobutamina a dosi crescenti (5, 10, 15{gamma}/kg/min). Per ogni paziente sono stati considerati 16 segmenti del ventricolo sinistro. Nel totale di 416 segmenti miocardici considerati l'analisi qualitativa del movimento parietale con ecocardiografia con dobutamina ha dimostrato 307 segmenti normocinetici, 64 cicatriziali e 45 vitali mentre con la cine Rm sono stati osservati rispettivamente 302 segmenti come normocinetici, 83 cicatriziali e 31 vitali. Tre mesi dopo l'intervento di rivascolarizzazione 15 pazienti sono stati rivalutati per verificare la ripresa contrattile dei segmenti giudicati vitali. L'esame ecocardiografico ha mostrato sensibilita' del 79% e specificita' del 97% mentre per l'analisi qualitativa mediante cine RM la sensibilita' e' stata del 96% e la specificita' dell'86%. Nei pazienti con infarto anterosettale l

  18. CD4+ T-cell clones obtained from cattle chronically infected with Fasciola hepatica and specific for adult worm antigen express both unrestricted and Th2 cytokine profiles.

    Science.gov (United States)

    Brown, W C; Davis, W C; Dobbelaere, D A; Rice-Ficht, A C

    1994-01-01

    The well-established importance of helper T (Th)-cell subsets in immunity and immunoregulation of many experimental helminth infections prompted a detailed study of the cellular immune response against Fasciola hepatica in the natural bovine host. T-cell lines established from two cattle infected with F. hepatica were characterized for the expression of T-cell surface markers and proliferative responses against F. hepatica adult worm antigen. Parasite-specific T-cell lines contained a mixture of CD4+, CD8+, and gamma/delta T-cell-receptor-bearing T cells. However, cell lines containing either fewer than 10% CD8+ T cells or depleted of gamma/delta T cells proliferated vigorously against F. hepatica antigen, indicating that these T-cell subsets are not required for proliferative responses in vitro. Seventeen F. hepatica-specific CD4+ Th-cell clones were examined for cytokine expression following concanavalin A stimulation. Biological assays to measure interleukin-2 (IL-2) or IL-4, gamma interferon (IFN-gamma), and tumor necrosis factor and Northern (RNA) blot analysis to verify the expression of IL-2, IL-4, and IFN-gamma revealed that the Th-cell clones expressed a spectrum of cytokine profiles. Several Th-cell clones were identified as Th2 cells by the strong expression of IL-4 but little or no IL-2 or IFN-gamma mRNA. The majority of Th-cell clones were classified as Th0 cells by the expression of either all three cytokines or combinations of IL-2 and IL-4 or IL-4 and IFN-gamma. No Th1-cell clones were obtained. All of the Th-cell clones expressed a typical memory cell surface phenotype, characterized as CD45Rlow, and all expressed the lymph node homing receptor (L selectin). These results are the first to describe cytokine responses of F. hepatica-specific T cells obtained from infected cattle and extend our previous analysis of Th0 and Th1 cells from cattle immune to Babesia bovis (W. C. Brown, V. M. Woods, D. A. E. Dobbelaere, and K. S. Logan, Infect. Immun. 61

  19. Vaccination against Fasciola hepatica using cathepsin L3 and B3 proteases delivered alone or in combination.

    Science.gov (United States)

    Wesołowska, Agnieszka; Basałaj, Katarzyna; Norbury, Luke J; Sielicka, Alicja; Wędrychowicz, Halina; Zawistowska-Deniziak, Anna

    2018-01-30

    No licensed vaccine is currently available for prevention of Fasciola hepatica infections. However, considering the alarming increase in drug resistance, there is an urgent need for a safe and fully effective vaccine against fasciolosis. Here, we tested if cathepsins L (FhCL3-1, FhCL3-2) and B (FhCB3) secreted by juvenile liver flukes are viable vaccine targets when delivered alone or in combination in a rat model. Since control over the early immune response is crucial for parasite's establishment in its host, it was hypothesised that targeting fluke juvenile stages may prove beneficial. Moreover, it was assumed that selected antigens will act in a cumulative manner to interfere with liver fluke migration and thereby will reduce F. hepatica infection. Recombinant FhCL3-1 and FhCL3-2 delivered alone reduced liver fluke burdens by 47 % and 63 %, respectively. A trivalent vaccine containing rFhCL3-1/CL3-2/CB3 did not increase the protective vaccine efficacy compared to the rFhCL3-2 vaccinated group (53 %), although, reductions in liver fluke wet weight (statistically significant) and liver damage score were most pronounced. Further, the highest IgG1 and IgG2a levels were seen in rFhCL3-2 vaccinated rats, the group for which the highest reduction in worm burden was demonstrated. Moreover, IgG1 and IgG2a levels in vaccinated rats were significantly elevated compared to those reported for control groups up to 4 week post-infection. While the mechanism of protection remains unknown, it appears that it depends on vaccine-induced antibodies directed against cathepsins. The obtained results imply that F. hepatica juvenile-specific cathepsins are promising vaccine candidates that induce responses that successfully target early migratory liver fluke stages. Now, the challenge is to evaluate these juvenile-specific cathepsins for use in livestock. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems.

    Science.gov (United States)

    Vicente, Belén; López-Abán, Julio; Rojas-Caraballo, Jose; del Olmo, Esther; Fernández-Soto, Pedro; Muro, Antonio

    2016-04-18

    Schistosomiasis is a water-borne disease afflicting over 261 million people in many areas of the developing countries with high morbidity and mortality. The control relies mainly on treatment with praziquantel. Fatty acid binding proteins (FABP) have demonstrated high levels of immune-protection against trematode infections. This study reports the immunoprotection induced by cross-reacting Fasciola hepatica FABP, native (nFh12) and recombinantly expressed using two different expression systems Escherichia coli (rFh15) and baculovirus (rFh15b) against Schistosoma mansoni infection. BALB/c mice were vaccinated with native nFh12 or recombinant rFh15 and rFh15 FABP from F. hepatica formulated in adjuvant adaptation (ADAD) system with natural or chemical synthesised immunomodulators (PAL and AA0029) and then challenged with 150 cercariae of S. mansoni. Parasite burden, hepatic lesions and antibody response were studied in vaccination trials. Furthermore differences between rFh15 and rFh15b immunological responses (cytokine production, splenocyte population and antibody levels) were studied. Vaccination with nFh12 induced significant reductions in worm burden (83%), eggs in tissues (82-92%) and hepatic lesions (85%) compared to infected controls using PAL. Vaccination with rFh15 showed lower total worm burden (56-64%), eggs in the liver (21-61%), eggs in the gut (30-77%) and hepatic damage (67-69%) using PAL and AA0029 as immunomodulators. In contrast, mice vaccinated with rFh15b showed only reductions in eggs trapped in the liver and intestine (53 and 60%, respectively), and hepatic lesions (45%). We observed a significant rise in TNFα, IL-6, IL-2, IL-4 and high antibody response (IgG, IgG1, IgG2a, IgM and IgE) in mice immunised with either rFh15 or rFh15b. Moreover, mice immunised with rFh15b showed an increase in IFNγ and a decrease in B220 cells compared to untreated mice, and less production of IgG1 and IgM than in mice immunised by rFh15. Higher level of

  1. Coproantígenos de Fasciola hepatica de posible utilidad en el diagnóstico de la fascioliasis

    Directory of Open Access Journals (Sweden)

    Ana M. Espino

    2000-04-01

    Full Text Available En el presente estudio se describen cuáles son los antígenos de Fasciola hepatica que están presentes en las heces de pacientes con fascioliasis crónica y de ratas infectadas experimentalmente con metacercarias de F. hepatica. Mediante el empleo de la técnica de inmunoelectrotransferencia (Western blot con un suero hiperinmune obtenido frente a antígenos de excreción/secreción de adultos de F. hepatica, se pudo demostrar que en las heces de los pacientes se encuentran antígenos de posible interés para el diagnóstico con masas moleculares de 14, 19, 20, 23, 25, 32, 46, 51 y 62 kilodaltons (kDa. Además, se comprobó que algunos de estos péptidos (los de 14, 20, 23 y 51 kDa son reconocidos también por la mayoría de los sueros de pacientes crónicos. Después de purificar por cromatografía de afinidad los antígenos presentes en heces de ratas con 6 a 12 semanas de infección, empleando para ello el anticuerpo monoclonal ES78 acoplado a Sepharosa 4B-CNBr, se identificaron seis polipéptidos de 11, 14, 26, 32, 47 y 51 kDa; otros tres polipéptidos, de 17, 24 y 66 kDa, solo se pudieron identificar en heces de ratas con 10 a 12 semanas de infección. Nuestros resultados sugieren que estos polipéptidos pueden ser antígenos comunes a ambos estadios parasitarios y constituir importantes marcadores de la fascioliasis aguda y crónica, en particular los de 14, 24, 26 y 51 kDa, puesto que reaccionaron con el inmunosuero, los sueros humanos y el anticuerpo monoclonal ES78.

  2. [Evaluation of an ELISA test with Fasciola hepatica metabolic antigen for diagnosis of human fascioliasis in Cajamarca, Peru].

    Science.gov (United States)

    Cornejo, Hernán; Oblitas, Fátima; Cruzado, Sandro; Quispe, William

    2010-01-01

    Metabolic (excretion/secretion) antigen was obtained from sheep infected with Fasciola hepatica, with a 1005 μg/μL of protein concentration, composed principally by proteins of molecular weight between 1.2 and 170 KDa. Bands of 170, 150, 31, 24, 18-14 and 10 kDa were detected. With this antigen an ELISA test was developed and the cut off was determined in 0.140. We evaluated 33 serums of patient with fascioliasis confirmed by visualization of eggs in feces, 177 serums of persons without fascioliasis from endemic rural areas of Cajamarca and 88 serums of patients with others parasitic and bacterial infections. We found a 97.0% of sensitivity, 96.6 specificity, 78.1% predictive positive value, 99.6 % predictive negative value. In 9/88 serums was found cross reactions. We recommended the implementation and use of this test for the fascioliasis diagnosis.

  3. High occurrence of Calodium hepaticum (syn. Capillaria hepatica spurious infection in a village in the Atlantic Forest of southern Brazil

    Directory of Open Access Journals (Sweden)

    Débora do Rocio Klisiowicz

    2014-06-01

    Full Text Available Calodium hepaticum (syn. Capillaria hepatica is a nematode of the Capillariidae family that infects rodents and other mammals. In Brazil, human spurious infections of C. hepaticum have been detected in indigenous or rural communities from the Amazon Basin, but not in the southern states of the country. Here, we report the highest occurrence (13.5% of 37 residents of C. hepaticum human spurious infection detected in Brazil and the first record in a southern region, Guaraqueçaba. The finding is explained by the area being located in the Atlantic Forest of the state of Paraná, surrounded by preserved forests and because the inhabitants consume the meat of wild mammals.

  4. Insights into the biological features of the antigenic determinants recognized by four monoclonal antibodies in redia and adult stages of the liver fluke Fasciola hepatica.

    Science.gov (United States)

    Alba, Annia; Sánchez, Jorge; Hernández, Hilda; Mosqueda, Maryani; Rodríguez, Suanel Y; Capó, Virginia; Otero, Oscar; Alfonso, Carlos; Marcet, Ricardo; Sarracent, Jorge

    2016-09-01

    Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES Ag) of adult parasites, which is a biologically-relevant mixture of molecules with functional roles during infection and immune evasion on definitive hosts. In the present report we describe the partial characterization of the epitopes recognized by these Mabs by heat treatment, mercaptoethanol reduction, pronase proteolysis and sodium peryodate oxidation, which suggested their predominant protein and conformational nature. Also, a comparative study using immunodetection assays on crude extracts and on histological sections of both rediae and adults of F. hepatica were performed to explore the expression pattern of the antigenic determinants in these developmental stages. From these experiments it was found that the Mabs reacted most likely with the same proteins of approximately 64 and 105 kDa present on both rediae and adult's extracts. However, the 1E4, 6G11 and 4E5 Mabs also recognized other molecules of the total extract of F. hepatica adults, a fact that constitutes an evidence of the antigenic variation between both stages and points at a certain biological relevance of the recognized antigenic determinants. Immunolocalization studies on histological sections revealed that all Mabs reacted with the tegument of F. hepatica in both rediae and adults stages, while the epitopes recognized by 1E4, 6G11 and 4E5 antibodies were also preferentially localized in the intestinal caeca and in different organs of the reproductive system of adult specimens. The immunogenicity of these antigenic determinants, their conserved status among different stages of the life cycle of F. hepatica and their presence in both tegument and ES Ag of adult parasites

  5. A 2.9 kDa Fasciola hepatica-recombinant protein based ELISA test for the detection of current-ovine fasciolosis trickle infected.

    Science.gov (United States)

    Arias, M; Hillyer, G V; Sánchez-Andrade, R; Suárez, J L; Pedreira, J; Lomba, C; Díaz, P; Morrondo, P; Díez-Baños, P; Paz-Silva, A

    2006-04-15

    The suitability of an enzyme linked immunosorbent assay (ELISA) test with a 2.9 kDa Fasciola hepatica-recombinant protein (FhrAPS) for diagnosing early and current-ovine fasciolosis was analyzed, and compared to that obtained by using a direct ELISA for detecting F. hepatica-circulating FhES antigens and to the coprological sedimentation for fluke egg quantitation. Fourteen Gallega autochthonous breed sheep were experimentally infected with metacercariae by a trickle system (small repetitive infections) and divided into two groups: G-I represented a primary infection for 34 weeks; G-R, animals with primary infection and reinfected 18 w.a.p.i. Seven sheep were left uninfected as the control group (G-C). Serum IgG antibody values against the FhrAPS rose rapidly by 1st w.a.p.i. in all infected sheep. Antibody levels in those with primary infection (G-I, G-C) peaked at 10 weeks, diminishing slightly and levelling from 16 to 34 weeks. Those with primary infection reinfected at 18 weeks had a rebound effect with the highest values observed. Circulating F. hepatica-ES antigens were detected by the 1st w.a.p.i. in all infected groups peaking at 6 weeks, decreasing rapidly to uninfected control values by 10 weeks of infection. Faecal egg-output started 11 weeks after primary infection. An increase in the IgG antibody as well as antigen responses to the FhrAPS and to anti-FhES from the 18 w.a.p.i. was recorded in G-T and G-R after the challenge infection. Antibody levels remained high whereas antigenemia values diminished after 6 weeks. A positive significant correlation between the IgG response against the FhrAPS and the F. hepatica circulating antigens (r2 = 0.428, p = 0.001) was obtained. In conclusion, our standardized diagnostic ELISA for fasciolosis based on the detection of IgG responses to the FhrAPS would be a valuable tool to diagnosis early and current F. hepatica-infections in sheep.

  6. Stimulating Neoblast-Like Cell Proliferation in Juvenile Fasciola hepatica Supports Growth and Progression towards the Adult Phenotype In Vitro.

    Science.gov (United States)

    McCusker, Paul; McVeigh, Paul; Rathinasamy, Vignesh; Toet, Hayley; McCammick, Erin; O'Connor, Anna; Marks, Nikki J; Mousley, Angela; Brennan, Gerard P; Halton, David W; Spithill, Terry W; Maule, Aaron G

    2016-09-01

    Fascioliasis (or fasciolosis) is a socioeconomically important parasitic disease caused by liver flukes of the genus Fasciola. Flukicide resistance has exposed the need for new drugs and/or a vaccine for liver fluke control. A rapidly improving 'molecular toolbox' for liver fluke encompasses quality genomic/transcriptomic datasets and an RNA interference platform that facilitates functional genomics approaches to drug/vaccine target validation. The exploitation of these resources is undermined by the absence of effective culture/maintenance systems that would support in vitro studies on juvenile fluke development/biology. Here we report markedly improved in vitro maintenance methods for Fasciola hepatica that achieved 65% survival of juvenile fluke after 6 months in standard cell culture medium supplemented with 50% chicken serum. We discovered that this long-term maintenance was dependent upon fluke growth, which was supported by increased proliferation of cells resembling the "neoblast" stem cells described in other flatworms. Growth led to dramatic morphological changes in juveniles, including the development of the digestive tract, reproductive organs and the tegument, towards more adult-like forms. The inhibition of DNA synthesis prevented neoblast-like cell proliferation and inhibited growth/development. Supporting our assertion that we have triggered the development of juveniles towards adult-like fluke, mass spectrometric analyses showed that growing fluke have an excretory/secretory protein profile that is distinct from that of newly-excysted juveniles and more closely resembles that of ex vivo immature and adult fluke. Further, in vitro maintained fluke displayed a transition in their movement from the probing behaviour associated with migrating stage worms to a slower wave-like motility seen in adults. Our ability to stimulate neoblast-like cell proliferation and growth in F. hepatica underpins the first simple platform for their long-term in vitro study

  7. Fasciola hepatica: effect of the natural light level on cercarial emergence from temperature-challenged Galba truncatula

    Directory of Open Access Journals (Sweden)

    Vignoles Philippe

    2014-01-01

    Full Text Available As abrupt changes in water temperature (thermal shock triggered a significantly greater cercarial emergence of Fasciola hepatica from experimentally infected Galba truncatula, laboratory investigations were carried out to study the influence of light on cercarial emergence in snails subjected to a thermal shock every week (a mean of 12 °C for 3 h during the patent period. Thermal shock for these temperature-challenged (TC snails was carried out outdoors under artificial or natural light, or indoors under constant artificial light. Compared with the infected control snails always reared indoors at 20 °C, the number of cercariae in TC snails subjected to a thermal shock and natural light outdoors was significantly greater. The repetition of this experiment by subjecting TC snails to the same thermal shock indoors under an artificial light level ranging from 600 to 3000 lux did not show any significant difference among the numbers of cercariae in the different subgroups. A detailed analysis of the results noted in the TC snails subjected to natural light during the thermal shock demonstrated that the number of cercariae-releasing snails was significantly higher between 601 and 1200 lux and for the highest nebulosity values (7–8 octas, which corresponds to a sufficiently or completely overcast sky. Contrary to the intensity of artificial light, which did not influence cercarial emergence, the natural light level had a significant effect on this process when F. hepatica-infected snails were subjected to a regular thermal shock during the patent period.

  8. Stimulating Neoblast-Like Cell Proliferation in Juvenile Fasciola hepatica Supports Growth and Progression towards the Adult Phenotype In Vitro

    Science.gov (United States)

    Rathinasamy, Vignesh; Toet, Hayley; McCammick, Erin; O’Connor, Anna; Marks, Nikki J.; Mousley, Angela; Brennan, Gerard P.; Halton, David W.; Spithill, Terry W.; Maule, Aaron G.

    2016-01-01

    Fascioliasis (or fasciolosis) is a socioeconomically important parasitic disease caused by liver flukes of the genus Fasciola. Flukicide resistance has exposed the need for new drugs and/or a vaccine for liver fluke control. A rapidly improving ‘molecular toolbox’ for liver fluke encompasses quality genomic/transcriptomic datasets and an RNA interference platform that facilitates functional genomics approaches to drug/vaccine target validation. The exploitation of these resources is undermined by the absence of effective culture/maintenance systems that would support in vitro studies on juvenile fluke development/biology. Here we report markedly improved in vitro maintenance methods for Fasciola hepatica that achieved 65% survival of juvenile fluke after 6 months in standard cell culture medium supplemented with 50% chicken serum. We discovered that this long-term maintenance was dependent upon fluke growth, which was supported by increased proliferation of cells resembling the “neoblast” stem cells described in other flatworms. Growth led to dramatic morphological changes in juveniles, including the development of the digestive tract, reproductive organs and the tegument, towards more adult-like forms. The inhibition of DNA synthesis prevented neoblast-like cell proliferation and inhibited growth/development. Supporting our assertion that we have triggered the development of juveniles towards adult-like fluke, mass spectrometric analyses showed that growing fluke have an excretory/secretory protein profile that is distinct from that of newly-excysted juveniles and more closely resembles that of ex vivo immature and adult fluke. Further, in vitro maintained fluke displayed a transition in their movement from the probing behaviour associated with migrating stage worms to a slower wave-like motility seen in adults. Our ability to stimulate neoblast-like cell proliferation and growth in F. hepatica underpins the first simple platform for their long-term in

  9. The effect of laser and X-rays on the invasive capability of the larvae of Fasciola hepatica L

    International Nuclear Information System (INIS)

    Bielecki, A.

    1986-01-01

    The material comprised eggs and larvae of F. hepatica and its hosts: intermediate - Galba truncatula (L.) and final (Mus. sp.). The eggs and larvae of F. hepatica were exposed to X-rays hard, 240 KV 20 R/s intensity and L-rays of a laser HE-Ne of low power 0.82 mW, light wave length 632.8 mm. The eggs were exposed at the first day of culture while the miracidia at the first hour of their life, using the doses of 1, 100, 200, 400, 600, 800, 1200 R in the case of X-rays and 5, 10, 15 and 30 min in the case of L-rays. In order to express the effect of irradiation on the invasive capability of the miracidia developing inside egg capsules those hatching of the eggs and of the adolescariae, the invasion index was calculated. In the case of miracidia all the doses of X-rays used in the experiment limit the number of adolescariae and adult flukes. The X-rays used at low doses in the case of adolescariae (1 R) increase the intensity of mouse infection with adult flukes, while higher doses (e.g. 1200 R) decrease the infection. The L-rays applied to miracidia cause an inrease in the number of cercariae developing per snail and the intensity of mouse infection also grows. In the case of adolescariae they decrease the intensity of mouse infection. In the flukes developing from larvae (miracidia, adolescariae) exposed to X- and L-rays the lack of eggs in the uterus was observed as well as the decrease of size. 43 refs., 5 figs., 6 tabs. (author)

  10. Stimulating Neoblast-Like Cell Proliferation in Juvenile Fasciola hepatica Supports Growth and Progression towards the Adult Phenotype In Vitro.

    Directory of Open Access Journals (Sweden)

    Paul McCusker

    2016-09-01

    Full Text Available Fascioliasis (or fasciolosis is a socioeconomically important parasitic disease caused by liver flukes of the genus Fasciola. Flukicide resistance has exposed the need for new drugs and/or a vaccine for liver fluke control. A rapidly improving 'molecular toolbox' for liver fluke encompasses quality genomic/transcriptomic datasets and an RNA interference platform that facilitates functional genomics approaches to drug/vaccine target validation. The exploitation of these resources is undermined by the absence of effective culture/maintenance systems that would support in vitro studies on juvenile fluke development/biology. Here we report markedly improved in vitro maintenance methods for Fasciola hepatica that achieved 65% survival of juvenile fluke after 6 months in standard cell culture medium supplemented with 50% chicken serum. We discovered that this long-term maintenance was dependent upon fluke growth, which was supported by increased proliferation of cells resembling the "neoblast" stem cells described in other flatworms. Growth led to dramatic morphological changes in juveniles, including the development of the digestive tract, reproductive organs and the tegument, towards more adult-like forms. The inhibition of DNA synthesis prevented neoblast-like cell proliferation and inhibited growth/development. Supporting our assertion that we have triggered the development of juveniles towards adult-like fluke, mass spectrometric analyses showed that growing fluke have an excretory/secretory protein profile that is distinct from that of newly-excysted juveniles and more closely resembles that of ex vivo immature and adult fluke. Further, in vitro maintained fluke displayed a transition in their movement from the probing behaviour associated with migrating stage worms to a slower wave-like motility seen in adults. Our ability to stimulate neoblast-like cell proliferation and growth in F. hepatica underpins the first simple platform for their long

  11. Immunological and pathophysiological studies on the infection of animals with liver specific trichocephalid Capillaris hepatica. Part a coordinated programme on isotopes and radiation in animal parasitology and immunology

    International Nuclear Information System (INIS)

    Zahner, H.

    1976-07-01

    The effect of X-ray radiation on the infection of animal liver with Capillaria hepatica was investigated. A dose of 70 Krd could not prevent an invasion of the liver with the parasite, and no differences in the number of lesions was found to occur between normal and irradiated parasite eggs. With regard to the fertility of the eggs, reproductivity was found to decrease after X-ray irradiation. Studies on cross- and histopathology were performed after infections with normal and irradiated (2 Krd and 5 Krd) eggs. Alterations of serum-enzyme activities were followed and the development of antibodies was demonstrated using an extract of non-embryonated C. hepatica eggs. Immunization experiments ascertained the immunizing capacity of a vaccine (as irradiated embryonated eggs) in comparison to normal infections and to intraperitoneal injections of nonembryonated eggs. The effect was determined with regard to the egg production of sublethal challenge infections and to the mortality after normally lethal challenge infections

  12. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica

    Czech Academy of Sciences Publication Activity Database

    Kráľová-Hromadová, I.; Špakulová, M.; Horáčková, Eva; Turčeková, Ĺ.; Novobilský, A.; Beck, R.; Koudela, Břetislav; Marinculić, A.; Rajský, D.; Pybus, M.

    2008-01-01

    Roč. 94, č. 1 (2008), s. 58-67 ISSN 0022-3395 R&D Projects: GA ČR GD524/03/H133; GA AV ČR IAA6022404 Grant - others:Slovak Research and Development Agency(SK) APVV-51-062205 Institutional research plan: CEZ:AV0Z60220518 Keywords : Fascioloides magna * Fasciola hepatica * ribosomal genes * mitochondrial genes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.165, year: 2008

  13. Higher physiopathogenicity by Fasciola gigantica than by the genetically close F. hepatica: experimental long-term follow-up of biochemical markers.

    Science.gov (United States)

    Valero, M Adela; Bargues, M Dolores; Khoubbane, Messaoud; Artigas, Patricio; Quesada, Carla; Berinde, Lavinia; Ubeira, Florencio M; Mezo, Mercedes; Hernandez, Jose L; Agramunt, Veronica H; Mas-Coma, Santiago

    2016-01-01

    Fascioliasis is caused by Fasciola hepatica and F. gigantica. The latter, always considered secondary in human infection, nowadays appears increasingly involved in Africa and Asia. Unfortunately, little is known about its pathogenicity, mainly due to difficulties in assessing the moment a patient first becomes infected and the differential diagnosis with F. hepatica. A long-term, 24-week, experimental study comparing F. hepatica and F. gigantica was made for the first time in the same animal model host, Guirra sheep. Serum biochemical parameters of liver damage, serum electrolytes, protein metabolism, plasma proteins, carbohydrate metabolism, hepatic lipid metabolism and inflammation were analysed on a biweekly basis as morbidity indicators. Serum anti-Fasciola IgG, coproantigen and egg shedding were simultaneously followed up. rDNA and mtDNA sequencing and the morphometric study by computer image analysis system (CIAS) showed that fasciolids used fitted standard species characteristics. Results demonstrated that F. gigantica is more pathogenic, given its bigger size and biomass but not due to genetic differences which are few. Fasciola gigantica shows a delayed development of 1-2 weeks regarding both the biliary phase and the beginning of egg shedding, with respective consequences for biochemical modifications in the acute and chronic periods. The higher F. gigantica pathogenicity contrasts with previous studies which only reflected the faster development of F. hepatica observed in short-term experiments. © The Author 2015. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  14. Studies on metabolic antigen of F. Hepatica. Part of a coordinated programme on isotopes and radiation in animal parasitology and immunology

    International Nuclear Information System (INIS)

    Cuperlovic, K.

    1977-06-01

    The final report submitted by Dr. Cuperlovic covers research findings on the immunological mechanisms and responses involved in liver fluke (Fasciola hepatica) infections in rabbits and sheep. Series of experiments were carried out to test the immunogenecity of labelled fluke tissue-fractions in rabbits. Hyperimmunization with secretory-excretory antigens gave conflicting results and it was concluded that only a very limited immunity is being conferred. Trials with anti-liver antibodies suggest that even autoimmune processes are involved

  15. Fasciola hepatica Surface Coat Glycoproteins Contain Mannosylated and Phosphorylated N-glycans and Exhibit Immune Modulatory Properties Independent of the Mannose Receptor.

    Directory of Open Access Journals (Sweden)

    Alessandra Ravidà

    2016-04-01

    Full Text Available Fascioliasis, caused by the liver fluke Fasciola hepatica, is a neglected tropical disease infecting over 1 million individuals annually with 17 million people at risk of infection. Like other helminths, F. hepatica employs mechanisms of immune suppression in order to evade its host immune system. In this study the N-glycosylation of F. hepatica's tegumental coat (FhTeg and its carbohydrate-dependent interactions with bone marrow derived dendritic cells (BMDCs were investigated. Mass spectrometric analysis demonstrated that FhTeg N-glycans comprised mainly of oligomannose and to a lesser extent truncated and complex type glycans, including a phosphorylated subset. The interaction of FhTeg with the mannose receptor (MR was investigated. Binding of FhTeg to MR-transfected CHO cells and BMDCs was blocked when pre-incubated with mannan. We further elucidated the role played by MR in the immunomodulatory mechanism of FhTeg and demonstrated that while FhTeg's binding was significantly reduced in BMDCs generated from MR knockout mice, the absence of MR did not alter FhTeg's ability to induce SOCS3 or suppress cytokine secretion from LPS activated BMDCs. A panel of negatively charged monosaccharides (i.e. GlcNAc-4P, Man-6P and GalNAc-4S were used in an attempt to inhibit the immunoregulatory properties of phosphorylated oligosaccharides. Notably, GalNAc-4S, a known inhibitor of the Cys-domain of MR, efficiently suppressed FhTeg binding to BMDCs and inhibited the expression of suppressor of cytokine signalling (SOCS 3, a negative regulator the TLR and STAT3 pathway. We conclude that F. hepatica contains high levels of mannose residues and phosphorylated glycoproteins that are crucial in modulating its host's immune system, however the role played by MR appears to be limited to the initial binding event suggesting that other C-type lectin receptors are involved in the immunomodulatory mechanism of FhTeg.

  16. Production of Monoclonal Antibody Against Excretory-Secretory Antigen of Fasciola hepatica and Evaluation of Its Efficacy in the Diagnosis of Fascioliasis.

    Science.gov (United States)

    Abdolahi Khabisi, Samaneh; Sarkari, Bahador; Moshfe, Abdolali; Jalali, Sedigheh

    2017-02-01

    Parasitological methods are not helpful for the diagnosis of fascioliasis in acute and invasive periods of the disease. Detection of coproantigens seems to be a suitable alternative approach in the diagnosis of fascioliasis. The present study aimed to develop a reliable antigen detection system, using monoclonal antibodies raised against excretory-secretory (ES) antigen of Fasciola hepatica, for the diagnosis of fascioliasis. Fasciola adult worms were collected from the bile ducts of infected animals. Species of the fluke was determined by polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR). ES antigen of F. hepatica was prepared. For production of monoclonal antibodies, mice were immunized with ES antigens of F. hepatica. Spleen cells from the immunized mice were fused with NS-1 myeloma cells, using polyethylene glycol. Hybridoma cells secreting specific antibody were expanded and cloned by limiting dilution. Moreover, polyclonal antibody was produced against F. hepatica ES antigen in rabbits. A capture enzyme-linked immunosorbent assay (ELISA) system, using produced monoclonal antibody, was designed and stool samples of infected animals along with control samples were tested by the system. The capture ELISA detected the coproantigen in 27 of 30 (90%) parasitologically confirmed fascioliasis cases, while 4 of 39 (10.25%) samples infected with other parasitic infections showed a positive reaction in this system. No positive reactivity was found with healthy control samples. Accordingly, sensitivity of 90% and specificity of 94.2% were obtained for the capture ELISA system. The results were compared with those obtained with commercial BIO-X ELISA, and a very good (kappa = 0.9) agreement was found between the commercial kit and the developed capture ELISA. Findings of this study showed that the produced monoclonal antibody has appropriate performance for the detection of Fasciola coproantigen in stool samples and can be appropriately

  17. Aptitude of Lymnaea palustris and L. stagnalis to Fasciola hepatica larval development through the infection of several successive generations of 4-mm-high snails.

    Science.gov (United States)

    Vignoles, P; Rondelaud, D; Dreyfuss, G

    2016-06-01

    Bimiracidial infections of Lymnaea palustris and Lymnaea stagnalis (shell height at exposure, 4 mm) with Fasciola hepatica were carried out during six successive snail generations to determine if prevalence and intensity of snail infection increased over time through descendants issuing from eggs laid by parents already exposed to this digenean. Controls were constituted by a French population of Galba truncatula (a single generation) infected according to the same protocol. In a first experiment performed with the F1 to F5 generations of L. palustris, the prevalence and intensity of F. hepatica infection in snails progressively increased. Immature rediae and a few cercariae-containing rediae of the digenean were observed in L. stagnalis from the F3 generation, but no free cercaria was noted in the bodies of this lymnaeid from the F4 to F6 generations. In another experiment carried out with the F6 generation of L. palustris, the prevalence of F. hepatica infection and the number of shed cercariae were significantly lower in L. palustris than in G. truncatula. This mode of snail infection suggests an explanation for cases of human fasciolosis occurring in central France after the collection of wild watercress from beds where L. palustris was the sole lymnaeid.

  18. STUDY OF THE PREVALENCE OF Capillaria hepatica IN HUMANS AND RODENTS IN AN URBAN AREA OF THE CITY OF PORTO VELHO, RONDÔNIA, BRAZIL

    Directory of Open Access Journals (Sweden)

    Elierson José Gomes da Rocha

    2015-02-01

    Full Text Available Introduction: Hepatic capillariosis, caused by Capillaria hepatica (Calodium hepaticum (Bancroft, 1893, Travassos, 1915 (Nematoda, Trichinelloidea, Capillariidae, is a common zoonosis in rodents but is rare in humans. Seventy-two cases in humans have been reported worldwide since the first case was described by MACARTHUR in 192417,27. This study aimed to determine the prevalence of Capillaria hepatica in humans and rodents in an urban area of Porto Velho, the capital of Rondônia, in Brazil. Methods: After conducting a census of the area, 490 residents were randomly selected, and, after signing a term of consent, provided blood samples that were screened for anti-Capillaria hepatica antibodies. Simultaneously, rats were captured to assess the prevalence of this parasite in rodents by histopathological examination in liver sections. Results: A prevalence of 1.8% was found among residents who had specific antibodies at a dilution of 1:150, indicating exposure to parasite eggs; 0.8% of the subjects also had positive titers at a dilution of 1:400, indicating true infection. The prevalence in rats was 2%. Conclusions: The prevalence of infection with this parasite among humans and rats was low. While the prevalence encountered among humans was within the limits reported in the literature, the prevalence among rodents was much lower.

  19. DNA sequence analyses reveal co-occurrence of novel haplotypes of Fasciola gigantica with F. hepatica in South Africa and Zimbabwe.

    Science.gov (United States)

    Mucheka, Vimbai T; Lamb, Jennifer M; Pfukenyi, Davies M; Mukaratirwa, Samson

    2015-11-30

    The aim of this study was to identify and determine the genetic diversity of Fasciola species in cattle from Zimbabwe, the KwaZulu-Natal and Mpumalanga provinces of South Africa and selected wildlife hosts from Zimbabwe. This was based on analysis of DNA sequences of the nuclear ribosomal internal transcribed spacer (ITS1 and 2) and mitochondrial cytochrome oxidase 1 (CO1) regions. The sample of 120 flukes was collected from livers of 57 cattle at 4 abattoirs in Zimbabwe and 47 cattle at 6 abattoirs in South Africa; it also included three alcohol-preserved duiker, antelope and eland samples from Zimbabwe. Aligned sequences (ITS 506 base pairs and CO1 381 base pairs) were analyzed by neighbour-joining, maximum parsimony and Bayesian inference methods. Phylogenetic trees revealed the presence of Fasciola gigantica in cattle from Zimbabwe and F. gigantica and Fasciola hepatica in the samples from South Africa. F. hepatica was more prevalent (64%) in South Africa than F. gigantica. In Zimbabwe, F. gigantica was present in 99% of the samples; F. hepatica was found in only one cattle sample, an antelope (Hippotragus niger) and a duiker (Sylvicapra grimmia). This is the first molecular confirmation of the identity Fasciola species in Zimbabwe and South Africa. Knowledge on the identity and distribution of these liver flukes at molecular level will allow disease surveillance and control in the studied areas. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Excretory/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine "BOMA" macrophages.

    Science.gov (United States)

    Bąska, Piotr; Norbury, Luke James; Zawistowska-Deniziak, Anna; Wiśniewski, Marcin; Januszkiewicz, Kamil

    2017-10-01

    Fasciola hepatica are trematodes that reside in the bile ducts of mammals. Infection causes US$3 billion in losses annually in animal production and is considered a zoonosis of growing importance. An under-represented area in F. hepatica research has been the examination of the different immunomodulatory abilities of various parasite isolates on the host immune system. In this paper, this issue was explored, with the bovine macrophage cell line "BOMA". The cells were matured by LPS treatment and stimulated with excretory/secretory antigens (ES) from two Fasciola hepatica isolates: a laboratory isolate "Weybridge" (Fh-WeyES) and a wild isolate (Fh-WildES). As expected, stimulation with antigen mixtures with highly similar compositions resulted in mild transcriptomic differences. However, there were significant differences in cytokine levels. Compared to Fh-WeyES, exposure to Fh-WildES upregulated 27 and downregulated 30 genes. Fh-ES from both isolates diminished the release of TNF-α, whereas only Fh-WildES decreased IL-10 secretion. Neither Fh-WeyES nor Fh-WildES had an impact on IL-12 release. Our results indicate that various isolates can have different immunomodulatory abilities and impacts on the bovine immune system.

  1. Fascioliasis in relatives of patients with Fasciola hepatica infection in Peru Fasciolosis en familiares de pacientes con infección por Fasciola hepatica en el Perú

    Directory of Open Access Journals (Sweden)

    Luis Marcos

    2005-08-01

    Full Text Available High prevalence rates of human fascioliasis have been described in several regions of Peru. We surveyed 20 families in an endemic area of Peru in order to determine the proportion of infection with F. hepatica in relatives of diagnosed subjects and in order to identify associated risk factors. The study included feces and blood samples of 93 subjects. Ages ranged from one to 53 (mean = 18.6; SD = 14.2. The overall prevalence of fascioliasis by fecal examinations was 33.3% (n = 83 and by serology, 51.9% (n = 86. The prevalence in age group I ( 19 years old 15.4% and 37.5%. The main associated risk factor with fascioliasis was eating salads (OR = 3.29, CI = 1.2 - 9.0, p = 0.02. In conclusion, human fascioliasis is highly prevalent in the relatives of index cases and the most significant risk factor of acquiring fascioliasis in the family is eating salads in endemic areas.Altas tasas de fasciolosis humana han sido descritas en varias regiones del Perú. Estudiamos 20 familias en una área endémica del Perú para determinar la proporción de infección con F. hepatica en los familiares de los sujetos diagnosticados y para identificar factores de riesgo asociados. El estudio incluyó un total de 93 sujetos, quienes contribuyeron con muestras de heces y sangre. Las edades comprendieron desde 1 a 53 años (media = 18.6; DS = 14.2. La prevalencia general de fasciolosis por exámenes de heces fue 33.3% (n = 83 y por serología, 51.9% (n = 86. La prevalencia en el grupo de edad I ( 19 años de edad 15.4% y 37.5%. El principal factor de riesgo asociado con fasciolosis fue comer ensaladas (OR = 3.29, IC = 1.2-9.0, p = 0.02. En conclusión, la fasciolosis humana es altamente prevalente en familiares de los casos índices y el factor de riesgo más significante para adquirirla en la familia es comer ensaladas en las áreas endémicas.

  2. Herd-level seroprevalence of Fasciola hepatica and Ostertagia ostertagi infection in dairy cattle population in the central and northeastern Poland.

    Science.gov (United States)

    Kowalczyk, Sławomir J; Czopowicz, Michał; Weber, Corinna N; Müller, Elisabeth; Nalbert, Tomasz; Bereznowski, Andrzej; Kaba, Jarosław

    2018-04-17

    Fasciola hepatica and Ostertagia ostertagi infections are widespread in cattle population of Europe, however data on their prevalence in Poland are only fragmentary. Therefore, the cross-sectional study was carried out to determine the herd-level seroprevalence of F. hepatica and O. ostertagi infection in dairy cattle population in the central and north-eastern provinces Poland, and to identify basic local risk factors for these infections. In total, 598 herds were enrolled, 394 (65.9%) in the north-eastern province and 204 (34.1%) in the central province. In each herd the questionnaire survey was conducted and bulk-tank milk (BTM) sample was collected and screened using two indirect immunoenzymatic tests. Optical density ratio (ODR) was regarded as the quantitative proxy of exposure to either of the two parasites. Both Fasciola and Ostertagia ELISA ODR in the north-eastern province was significantly higher than ODR in the central province. At the cut-off value of ODR = 0.27 the true herd-level seroprevalence of F. hepatica was 79.6% (95% CI: 74.0%, 84.3%) in the north-eastern province and 13.0% (95% CI: 5.3%, 21.7%) in the central province. At the cut-off of ODR = 0.50151 of 188 herds (80.3%, 95% CI: 74.1%, 85.4%) were seropositive for O. ostertagi in the north-eastern province and only 70 of 136 herds (51.5%, 95% CI: 43.1%, 59.7%) were seropositive in the central province. Location of a herd in the north-eastern province, longer grazing period practiced in a herd and > 50%-share of grazing grass in roughage were all positively related to the increase in exposure to both parasites. Moreover, the use of hay or haylage as main roughage proved to be positively related to the increase in exposure to F. hepatica. F. hepatica and O. ostertagi are widespread in cattle population in Poland, however their occurrence at a herd-level varies between different regions of Poland. This diversity can only partly be explained by different herd management, and appears

  3. Fasciola hepatica EN GANADO BOVINO DE CARNE EN SIQUIRRES Y LESIONES ANATOMO-HISTOPATOLÓGICAS DE HÍGADOS BOVINOS DECOMISADOS EN MATADEROS DE COSTA RICA

    Directory of Open Access Journals (Sweden)

    Carlos Ernesto Alpízar

    2013-01-01

    Full Text Available El presente estudio fue realizado con el objetivo de determinar la infección por Fasciola hepatica (F. hepatica en una finca dedicada al ganado de carne en el cantón de Siquirres, provincia de Limón para caracterizar las lesiones más comunes de hígados parasitados, en 3 plantas de sacrificio de bovinos del país. De agosto del 2005 a agosto del 2006, fueron recolectadas muestras fecales de 577 bovinos de razas cebuinas con edades entre 6 meses y 2,5 años. Cada muestra fue sometida a la técnica coprológica de sedimentación; también se buscaron y recolectaron moluscos con el objetivo de identificar la(s especie(s involucrada(s en la transmisión del parásito en la finca. Asimismo, se recolectaron muestras de hígados bovinos infectados con el parásito en 3 mataderos del Valle Central, para el análisis histopatológico. F. hepatica fue diagnosticada en 67 (11,3% de los 577 bovinos evaluados, y el porcentaje de infección fue mayor en agosto del 2005 (31,6% y agosto del 2006 (26,2%. Además, los animales con edades entre 18 y 24 meses mostraron mayor frecuencia de infección (19,4%, mientras que los animales de 12 a menos de 18 meses mostraron la menor frecuencia de infección (2,4%. Los caracoles identificados fueron de la especie Thiara tuberculata, que no fue identificada como vector de F. hepatica. Las principales lesiones en los hígados decomisados fueron atrofia moderada de lóbulos hepáticos, aumento de consistencia, engrosamiento de ductos hepáticos con presencia de calcificación, material mucoso y formas adultas del parásito. Histológicamente corresponden a colangiohepatitis necrótica con formación de depósitos cálcicos distróficos e hiperplasia de ductos biliares. Con base en los resultados obtenidos se pudo determinar la presencia de F. hepatica en los bovinos de la finca y de la zona, con marcado incremento de la infección en la época lluviosa, además del hallazgo colateral de Paramphistomum spp.

  4. Determinación de la prevalencia de Fasciola hepatica en bovinos de Rio de Oro y Aguachica, Cesar

    Directory of Open Access Journals (Sweden)

    Juan David Estupiñán

    2016-06-01

    Full Text Available Introducción: La fasciolosis, es una zoonosis con distribución mundial provocada por Fasciola hepatica, la cual afecta principalmente a los rumiantes y accidentalmente el hombre puede ser un hospedero. Con mayor frecuencia afecta a los animales de regiones en las que llueve abundantemente, no obstante, puede aparecer en regiones más secas a lo largo de ríos o afluentes de riego. Objetivo: Determinar la prevalencia de F. hepatica en bovinos de Rio de Oro y Aguachica, Cesar mediante el análisis de materia fecal utilizando la técnica de sedimentación de Dennis modificado. Materiales y métodos: El estudio realizado fue de tipo descriptivo, de corte transversal. La población evaluada fue de 12.290 bovinos, correspondientes a la totalidad de bovinos de Rio de Oro y Aguachica. La muestra tomada correspondió a 905 bovinos, seleccionados bajo un único criterio, que fuesen mayores de 4 meses, sin importar sexo. Las muestras fueron recolectadas con todas las normas de bioseguridad veterinaria, se tomaron directamente del recto del animal con guante de palpación y fueron enviadas desde el lugar de origen hasta los laboratorios de la Universidad de Santander el mismo día; al recibir las muestras se preservaron con formalina al 10% para su procesamiento al día siguiente. Las muestras fueron diluidas con agua en un vaso desechable y se filtraron con gasa en un vaso de precipitado, completando el volumen hasta 500 mL con agua. Se dejó sedimentar por 30 minutos para descartar el volumen del sobrenadante y dejar únicamente el sedimento. Se repitió este paso con un tiempo de sedimentación de 15 minutos y finalmente otro con 5 minutos. Al terminar la sedimentación y descartar el volumen del sobrenadante, se recolectó el sedimento final en un frasco de orina, sedimento que se observa como una arenilla, allí fue donde se buscaron los huevos de F. hepatica, observándolos en su totalidad en una caja de Petri, utilizando para ello un estereoscopio

  5. Distribution and habitats of the snail Lymnaea truncatula, intermediate host of the liver fluke Fasciola hepatica, in South Africa

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    K.N. de Kock

    2003-07-01

    Full Text Available This paper focuses on the geographical distribution and habitats of Lymnaea truncatula, the intermediate, snail host of the liver fluke, Fasciola hepatica, as reflected by the 723 samples in the database of the National Freshwater Snail Collection, Potchefstroom, South Africa. The 221 different loci (1/16-degree squares on record reflect an extensive but discontinuous distribution, except in Lesotho and in parts of the Mpumalanga, Gauteng and North West provinces of South Africa. Although recorded from 12 different types of waterbody, it was mostly (42.0 % recovered from swamps. Most samples (45.8 % were collected in habitats with slow-flowing water. A muddy substratum was recorded for 62.5 % of the samples. Most samples (86.3 % were collected in habitats with a mean annual air temperature of 10-20 o C, and more than 69 % came from localities with a mean annual rainfall of 600-900mm. An integrated decision tree constructed from the data indicated that temperature and types of waterbody play a decisive role in determining the presence of L. truncatula in a given area. A temperature index calculated for all mollusc species ranked L. truncatula second in a total of 53 species according to its association with low temperatures. It remains to be established whether its distribution is indeed discontinuous, and whether its preference for a particular habitat, amphibious habits and ability to aestivate could have resulted in some populations having been overlooked during surveys.

  6. Rapid identification and differentiation of Fasciola hepatica and Fasciola gigantica by a loop-mediated isothermal amplification (LAMP) assay.

    Science.gov (United States)

    Ai, L; Li, C; Elsheikha, H M; Hong, S J; Chen, J X; Chen, S H; Li, X; Cai, X Q; Chen, M X; Zhu, X Q

    2010-12-15

    The present study developed and validated a species-specific loop-mediated isothermal amplification (LAMP) assay for the rapid detection and discrimination of Fasciola hepatica and Fasciola gigantica. The LAMP assay is inexpensive, easy to perform and shows rapid reaction, wherein the amplification can be obtained in 45 min under isothermal conditions of 61 °C or 62 °C by employing a set of four species-specific primer mixtures and results can be checked through naked-eye visualization. The optimal assay conditions with no cross-reaction with other closely related trematodes (Clonorchis sinensis, Opisthorchis viverrini, Orientobilharzia turkestanicum and Schistosoma japonicum) as well as within the two Fasciola species were established. The assay was validated by examining F. gigantica DNA in the intermediate host snails and in faecal samples. The results indicated that the LAMP assay is approximately 10(4) times more sensitive than the conventional specific PCR assays. These findings indicate that this Fasciola species-specific LAMP assay may have a potential clinical application for detection and differentiation of Fasciola species, especially in endemic countries. Copyright © 2010 Elsevier B.V. All rights reserved.

  7. Time-Course Study of the Transcriptome of Peripheral Blood Mononuclear Cells (PBMCs) from Sheep Infected with Fasciola hepatica

    Science.gov (United States)

    Scheerlinck, Jean-Pierre; Ansell, Brendan R. E.; Hall, Ross S.; Gasser, Robin B.; Jex, Aaron R.

    2016-01-01

    Fasciola hepatica is a parasitic trematode that infects a wide range of mammalian hosts, including livestock and humans, in temperate and tropical regions globally. This trematode causes the disease fascioliasis, which consists of an acute phase (≤ 12 weeks) during which juvenile parasites migrate through the host liver tissues, and a chronic phase (> 12 weeks) following the establishment of adult parasites in the liver bile ducts. Few studies have explored the progression of the host response over the course of Fasciola infection in the same animals. In this study, we characterized transcriptomic changes in peripheral blood mononuclear cells (PBMCs) collected from sheep at three time points over the first eight weeks of infection relative to uninfected controls. In total, 183 and 76 genes were found to be differentially transcribed at two and eight weeks post-infection respectively. Functional and pathway analysis of differentially transcribed genes revealed changes related to T-cell activation that may underpin a Th2-biased immune response against this parasite. This first insight into the dynamics of host responses during the early stages of infection improves the understanding of the pathogenesis of acute fascioliasis, informs vaccine development and presents a set of PBMC markers with diagnostic potential. PMID:27438474

  8. Antibody response in cattle, sheep and rats to infection with γ-irradiated metacercariae of Fasciola hepatica

    International Nuclear Information System (INIS)

    Hughes, D.L.; Doy, T.G.

    1982-01-01

    Cattle, sheep and rats were infected orally with γ-irradiated metacercariae of Fasciola hepatica, or with normal metacercariae. The antibody response was monitored in each host to metacercarial tegument (T0), juvenile tegument (T1), adult tegument (T2) and gut antigens. The response was examined at weekly intervals for cattle and sheep throughout 15 weeks of infection and four weeks after infection in rats, using an indirect fluorescent antibody labelling technique. It was found that the irradiated metacercariae engendered a normal humoral response to T0, T1 and gut antigens in all three hosts although the antibody levels were somewhat reduced due to early death or stunting of the flukes. T0 and T1 appeared to be antigenically similar. Antibodies against T2 appeared late in the animals infected with γ-irradiated metacercariae and the titres attained were considerably lower than in the controls. The T2 antigen stimulus in the animals given γ-irradiated metacercariae was probably provided by flukes which 'broke through' the developmental barrier imposed by irradiation and which were found alive at autopsy. (author)

  9. Time-Course Study of the Transcriptome of Peripheral Blood Mononuclear Cells (PBMCs from Sheep Infected with Fasciola hepatica.

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    Cristian A Alvarez Rojas

    Full Text Available Fasciola hepatica is a parasitic trematode that infects a wide range of mammalian hosts, including livestock and humans, in temperate and tropical regions globally. This trematode causes the disease fascioliasis, which consists of an acute phase (≤ 12 weeks during which juvenile parasites migrate through the host liver tissues, and a chronic phase (> 12 weeks following the establishment of adult parasites in the liver bile ducts. Few studies have explored the progression of the host response over the course of Fasciola infection in the same animals. In this study, we characterized transcriptomic changes in peripheral blood mononuclear cells (PBMCs collected from sheep at three time points over the first eight weeks of infection relative to uninfected controls. In total, 183 and 76 genes were found to be differentially transcribed at two and eight weeks post-infection respectively. Functional and pathway analysis of differentially transcribed genes revealed changes related to T-cell activation that may underpin a Th2-biased immune response against this parasite. This first insight into the dynamics of host responses during the early stages of infection improves the understanding of the pathogenesis of acute fascioliasis, informs vaccine development and presents a set of PBMC markers with diagnostic potential.

  10. Distribution of Fasciola hepatica and F. gigantica in the endemic area of Guilan, Iran: Relationships between zonal overlap and phenotypic traits.

    Science.gov (United States)

    Ashrafi, Keyhan; Valero, M Adela; Peixoto, Raquel V; Artigas, Patricio; Panova, Miroslava; Mas-Coma, Santiago

    2015-04-01

    Fascioliasis is a zoonotic disease emerging in numerous parts of the world. In any endemic area, the characterisation of scenarios and patterns of infection must always be considered the starting point before implementing any control measure. Fascioliasis is a parasitic disease of different epidemiological, pathological and control characteristics depending on the endemic area and the causal agent, Fasciola hepatica and Fasciolagigantica. Classically it has been accepted that F. hepatica is present worldwide, while the distribution of the two species overlaps in many areas of Africa and Asia. Fascioliasis caused by F. hepatica, F. gigantica and intermediate forms is present in Guilan province, a complicated epidemiological situation where the highest human infection rates have been described in Iran. Morphometric tools were used to analyse the possible relationship between liver-fluke metric traits and geographical and altitudinal distribution. This is the first study in which a detailed distribution of both Fasciola species is analysed in a human fascioliasis endemic area with a zonal overlap transmission pattern. An accurate analysis was conducted to phenotypically discriminate between fasciolids from naturally infected livestock (cattle, buffaloes, sheep and goats). The distribution of the % F. hepatica-like (F.h.) and F. gigantica-like (F.g.) flukes detected in each liver versus altitude (m) in each group was analysed. The presence of F.g. specimens mainly in locations below sea level (average: 11.23% F.h., 88.77% F.g.), the presence of both species with similar intensity at 1-99m (average: 56.95% F.h., 43.05% F.g.) and the presence of F.h. specimens mainly from 100 to 999m (average: 71.69% F.h., 28.31% F.g.) as well as in locations with an altitude above 1000m (average: 97.48% F.h., 2.52% F.g.) are noteworthy. A significant positive correlation was obtained between altitude and % F.h., and a significant negative correlation was obtained between altitude and

  11. Comparación de las técnicas Kato-Katz, TSET y TSR en el diagnóstico de infección por Fasciola hepatica en humanos

    OpenAIRE

    Nelson Uribe Delgado; Raúl Fernando Sierra Balcárcel; Cindy Tatiana Espinosa González

    2012-01-01

    Introducción: La Fasciolosis es una enfermedad causada por el parásito Fasciola hepatica, que afecta primordialmente a bovinos, ovinos, caprinos y en algunas zonas de forma endémica a los humanos. En la investigación clínica y epidemiológica de fasciolosis humana es importante contar con técnicas coprológicas de diagnóstico de alta sensibilidad y especificidad. Objetivo: realizar la comparación de tres técnicas coprológicas para el diagnóstico de infección por Fasciola hepatica en humanos. Me...

  12. Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

    Science.gov (United States)

    Nguyen, Khue Thi; Nguyen, Nga Thi Bich; Doan, Huong Thi Thanh; Le, Xuyen Thi Kim; Hoang, Chau Thi Minh; De, Nguyen Van

    2012-01-01

    A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of mitochondrial DNA (mtDNA) spanning the region of cox1-trnT-rrnL, two species-specific forward primers were designed, FHF (for F. hepatica) and FGF (for F. gigantica), and a single reverse primer, FHGR (common for both species). Conventional PCR followed by sequencing was applied using species-specific primer pairs to verify the specificity of primers and the identity of Fasciola DNA templates. Duplex PCR (using three primers) was used for testing with the DNA extracted from adult worms, miracidia, and eggs, producing amplicons of 1,031 bp for F. hepatica and 615 bp for F. gigantica. The duplex PCR failed to amplify from DNA of other common liver and intestinal trematodes, including two opisthorchiids, three heterophyids, an echinostomid, another fasciolid, and a taeniid cestode. The sensitivity assay showed that the duplex PCR limit of detection for each Fasciola species was between 0.012 ng and 0.006 ng DNA. Evaluation using DNA templates from 32 Fasciola samples (28 adults and 4 eggs) and from 25 field-collected stools of ruminants and humans revealed specific bands of the correct size and the presence of Fasciola species. This novel mtDNA duplex PCR is a sensitive and fast tool for accurate identification of Fasciola species in areas of distributional and zonal overlap. PMID:22692744

  13. Development and evaluation of a single-step duplex PCR for simultaneous detection of Fasciola hepatica and Fasciola gigantica (family Fasciolidae, class Trematoda, phylum Platyhelminthes).

    Science.gov (United States)

    Le, Thanh Hoa; Nguyen, Khue Thi; Nguyen, Nga Thi Bich; Doan, Huong Thi Thanh; Le, Xuyen Thi Kim; Hoang, Chau Thi Minh; De, Nguyen Van

    2012-08-01

    A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of mitochondrial DNA (mtDNA) spanning the region of cox1-trnT-rrnL, two species-specific forward primers were designed, FHF (for F. hepatica) and FGF (for F. gigantica), and a single reverse primer, FHGR (common for both species). Conventional PCR followed by sequencing was applied using species-specific primer pairs to verify the specificity of primers and the identity of Fasciola DNA templates. Duplex PCR (using three primers) was used for testing with the DNA extracted from adult worms, miracidia, and eggs, producing amplicons of 1,031 bp for F. hepatica and 615 bp for F. gigantica. The duplex PCR failed to amplify from DNA of other common liver and intestinal trematodes, including two opisthorchiids, three heterophyids, an echinostomid, another fasciolid, and a taeniid cestode. The sensitivity assay showed that the duplex PCR limit of detection for each Fasciola species was between 0.012 ng and 0.006 ng DNA. Evaluation using DNA templates from 32 Fasciola samples (28 adults and 4 eggs) and from 25 field-collected stools of ruminants and humans revealed specific bands of the correct size and the presence of Fasciola species. This novel mtDNA duplex PCR is a sensitive and fast tool for accurate identification of Fasciola species in areas of distributional and zonal overlap.

  14. Susceptibilidade de Lymnaea (pseudosuccinea columella (Say, 1817 exposta à infecção por miracídios de Fasciola hepatica (Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Alfredo Raimundo Corrêa Dacal

    1988-10-01

    Full Text Available Com o objetivo de estudar a susceptibilidade de Lymnaea (pseudosuccinea columella de Minas Gerais, Estado onde ainda não se registraram casos autóctones de fasciolose hepática, foram utilizadas as amostras MG 1, MG 2, MG 3 e MG 4, provenientes de diferentes regiões deste Estado, para estudo comparativo com as amostras RJ e RGS procedentes dos Estados do Rio de Janeiro e Rio Grande do Sul, áreas de focos de fasciolose. A amostra de Fasciola hepatica utilizada era procedente de ovinos abatidos no município de Camapuã, no Rio Grande do Sul, e foi mantida em coelhos. Os moluscos foram infectados com 6, 8 e 10 miracídos por molusco, sendo estudadas as incidências de infecção e de mortalidade. Nas condições que se realizou o trabalho diante dos resultados obtidos, foram tiradas as seguintes conclusões: 1. A mortalidade dos caramujos parece não estar diretamente relacionada com a percentagem de infecção; 2. As amostras de Lymnaea (pseudosuccinea columella de Minas Gerais, do Rio de Janeiro e do Rio Grande do Sul mostraram-se identicamente susceptíveis à infecção por Fasciola hepatica; 3. A população humana e os rebanhos bovino, ovino e suíno de Minas Gerais, particularmente aqueles do Vale do Rio Paraíba, podem ser considerados sujeitos à infecção por Fasciola hepatica.

  15. DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis.

    Science.gov (United States)

    Bargues, María Dolores; Gayo, Valeria; Sanchis, Jaime; Artigas, Patricio; Khoubbane, Messaoud; Birriel, Soledad; Mas-Coma, Santiago

    2017-02-01

    Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. A study including DNA marker sequencing of fasciolids and lymnaeids, an experimental study of the life cycle in Uruguay, and a review of human fascioliasis in Uruguay, are performed. The characterization of Fasciola hepatica from cattle and horses of Uruguay included the complete sequences of the ribosomal DNA ITS-2 and ITS-1 and mitochondrial DNA cox1 and nad1. ITS-2, ITS-1, partial cox1 and rDNA 16S gene of mtDNA were used for lymnaeids. Results indicated that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. The life cycle and transmission features of F. hepatica by L. neotropica of Uruguay were studied under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. On this baseline, we reviewed the 95 human fascioliasis cases reported in Uruguay and analyzed the risk of human infection in front of future climate change estimations. The correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock into Uruguay allowed to understand the molecular diversity detected. Although Uruguayan L. neotropica is a highly efficient vector, its transmission capacity is markedly lower than that of Bolivian G. truncatula. This allows to understand the transmission and epidemiological differences between Andean highlands and non

  16. Network Analysis of the Systemic Response to Fasciola hepatica Infection in Sheep Reveals Changes in Fibrosis, Apoptosis, Toll-Like Receptors 3/4, and B Cell Function

    Science.gov (United States)

    Fu, Yan; Browne, John A.; Killick, Kate; Mulcahy, Grace

    2017-01-01

    The Trematode Fasciola hepatica is an important cause of disease in livestock and in man. Modulation of immunity is a critical strategy used by this parasite to facilitate its long-term survival in the host. Understanding the underlying mechanisms at a system level is important for the development of novel control strategies, such as vaccination, as well as for increasing general understanding of helminth-mediated immunoregulation and its consequences. Our previous RNA sequencing work identified a large number of differentially expressed genes (DEG) from ovine peripheral blood mononuclear cells (PBMCs) at acute and chronic stages of F. hepatica infection, and yielded important information on host–parasite interaction, with particular reference to the immune response. To extend our understanding of the immunoregulatory effects of this parasite, we employed InnateDB to further analyze the DEG dataset and identified 2,458 and 224 molecular interactions in the context of innate immunity from the acute and chronic stages of infection, respectively. Notably, 458 interactions at the acute stage of infection were manually curated from studies involving PBMC-related cell-types, which guaranteed confident hypothesis generation. NetworkAnalyst was subsequently used to construct and visualize molecular networks. Two complementary strategies (function-first and connection-first) were conducted to interpret the networks. The function-first approach highlighted subnetworks implicated in regulation of Toll-like receptor 3/4 signaling in both acute and chronic infections. The connection-first approach highlighted regulation of intrinsic apoptosis and B-cell receptor-signaling during acute and chronic infections, respectively. To the best of our knowledge, this study is the first system level analysis of the regulation of host innate immunity during F. hepatica infection. It provides insights into the profound changes induced by F. hepatica infection that not only favors parasite

  17. Farm specific transmission patterns of Fasciola hepatica in Danish dairy cattle based on different diagnostic methods and monitoring of grazing management

    DEFF Research Database (Denmark)

    Takeuchi-Storm, Nao; Denwood, Matthew; Petersen, Heidi Huus

    levels in bulk tank milk were selected. From each farm a cohort of 40 animals from different age groups (calves, heifers, primiparous and multiparous cows) were sampled 7 times between April 2015 and January 2017. Diagnostic methods included faecal egg count by sedimentation, serum ELISA and coproantigen...... ELISA. Additionally, monthly bulk tank milk samples were analyzed by ELISA. The analyses are ongoing, but preliminary results indicate that F. hepatica is mainly transmitted via summer infection of snails as most animals seroconvert in late autumn without shedding of eggs. However, infection early...

  18. Recurrent back pain after diskectomy: MRI findings MR of the postoperative lumbar spine; Recurrencia del dolor lumbar en la columna operada: Hallazgos en Resonancia Magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Aparicio, Rocio; Eguren, Leonor Z; Schinder, Humberto; Stur, Mariela [CIMA, Sanatorio Mapaci, Rosario, Santa Fe (Argentina)

    2008-07-01

    Purpose: To show the morphological changes in postoperative lumbar spine. Material and methods: We reviewed 128 Magnetic Resonance Imaging (MRI) studies of lumbar spine. All patients complained of low back pain and had a previous lumbar surgery (1 month to 8 years). All the examinations include axial and sagittal Gadopentate-dimeglumine enhanced T1 weighted imaging. Results: Only 7,8% have the normal appearance of the postoperative lumbar spine. Epidural scar was found in 69% of examinations; of this group, 72% showed radicular involvement, and 28% had no radicular involvement. Recurrent disc herniation was found in 14,8% of exams, using gadopentate-dimeglumine to assist the differentiation from epidural fibrosis; infections like spondylodiscitis and abscess in 12,5%. Other findings included arachnoiditis, pseudomeningoceles and seromas. Conclusions: MRI is a useful method to study the postoperative lumbar spine, specially when enhanced with gadopentate-dimeglumine. Enhanced images can differentiate two of the major complication like epidural fibrosis, and recurrent disc herniation. Spondylodiscitis are important and frequent too. Although the findings of complications in our series in the postoperative lumbar spine represent 92,18 %, these not always explain the symptomatology. Therefore, the interrelation is important with the surgeon to determine which abnormalities are clinically significant. (authors) [Spanish] Objetivo: Mostrar los hallazgos evidenciados en Resonancia Magnetica (RM) en pacientes operados de columna lumbar. Material y metodos: Se evaluaron retrospectivamente 128 estudios de RM de columna lumbar. Todos los pacientes consultaron por dolor lumbar y tenian antecedentes quirurgicos de un tiempo de evolucion que variaba entre 1 mes y 8 anos. En todos los casos el protocolo de estudio incluyo secuencias axiales y sagitales T1 con gadolinio, ademas del protocolo habitual. Resultados: Solo el 7,8 % de los estudios mostro los hallazgos postoperatorios

  19. Disruption of egg production by triclabendazole-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (Mirazid).

    Science.gov (United States)

    Abdelaal, Mohamed M O; Brennan, Gerard P; Hanna, Robert E B; Abdel-Aziz, Ahmed; Fairweather, Ian

    2017-06-01

    An in vitro study has been carried out to monitor changes to the female reproductive system in adult triclabendazole (TCBZ)-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh ("Mirazid"). Flukes were immersed for 6 h and 24 h in myrrh extract at a concentration of 200 µg/ml, then processed for histological and transmission electron microscope (TEM) examination of the uterus, Mehlis' gland, ovary and vitellaria. Egg production had become abnormal at 6 h post-treatment (pt), with the uterine lumen being filled with free vitelline cells and masses of shell protein material; few eggs were present. At 24 h pt, no eggs were present. Distinct changes to the ovary and Mehlis' gland were only observed after 24 h incubation in Mirazid. The ovary contained numbers of apoptotic oogonia and oocytes. In the Mehlis' gland, the S1 cells were disorganised and the processes from them were vacuolated, although the disruption was not significant. More severe changes were observed in the vitelline cells and follicles. After 6 h incubation in Mirazid, although the gross organisation of the vitelline follicles appeared to be normal, nuclear changes indicative of the early stages of apoptosis were observed in the stem cells and shell protein production by the mature cells had decreased. At 24 h pt, a distinct shift in cell population was evident, with the follicles containing mainly mature cells and spaces were present between the cells. The shell globule clusters in the mature cells were disorganised. In more severely-affected follicles, cells were seen to be breaking down, with karyolytic nuclei and disintegrating cytoplasm. Overall, the results have shown that exposure to Mirazid treatment had a severe impact on egg production by TCBZ-resistant flukes, an effect that was mediated by disruption of the vitelline cells and of the mechanism co-ordinating egg formation in the ootype.

  20. Substrate Specificity of Cysteine Proteases Beyond the S2 Pocket: Mutagenesis and Molecular Dynamics Investigation of Fasciola hepatica Cathepsins L

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    Ileana Corvo

    2018-04-01

    Full Text Available Cysteine proteases are widespread in all life kingdoms, being central to diverse physiological processes based on a broad range of substrate specificity. Paralogous Fasciola hepatica cathepsin L proteases are essential to parasite invasion, tissue migration and reproduction. In spite of similarities in their overall sequence and structure, these enzymes often exhibit different substrate specificity. These preferences are principally determined by the amino acid composition of the active site's S2 subsite (pocket of the enzyme that interacts with the substrate P2 residue (Schetcher and Berger nomenclature. Although secreted FhCL1 accommodates aliphatic residues in the S2 pocket, FhCL2 is also efficient in cleaving proline in that position. To understand these differences, we engineered the FhCL1 S2 subsite at three amino acid positions to render it identical to that present in FhCL2. The substitutions did not produce the expected increment in proline accommodation in P2. Rather, they decreased the enzyme's catalytic efficiency toward synthetic peptides. Nonetheless, a change in the P3 specificity was associated with the mutation of Leu67 to Tyr, a hinge residue between the S2 and S3 subsites that contributes to the accommodation of Gly in S3. Molecular dynamic simulations highlighted changes in the spatial distribution and secondary structure of the S2 and S3 pockets of the mutant FhCL1 enzymes. The reduced affinity and catalytic efficiency of the mutant enzymes may be due to a narrowing of the active site cleft that hinders the accommodation of substrates. Because the variations in the enzymatic activity measured could not be exclusively allocated to those residues lining the active site, other more external positions might modulate enzyme conformation, and, therefore, catalytic activity.

  1. Action of sulfurous acid on pollen. [Hepatica triloba; Helleborus orientalis; Vinca minor; Viola tricolor; Primula officinalis; Lilium candidum; Petunia; Pisum; Helleborus viridus; Galanthus nivealis; Vinca major; Convallaria maialis; Narcissus poeticus; Caltha palustris; Cystisus laburnum; Orchis maculata; Bilbergia; Eranthus; Crocus

    Energy Technology Data Exchange (ETDEWEB)

    Sabachnikoff, V

    1912-01-23

    The following ornamental plants: Hepatica triloba, Helleborus orientalis, Vinca minor, Viola tricolor, Primula officinalis, Lilium candidum, Petunia, Pisum, Helleborus viridus, Galanthus nivealis, Vinca major, Convallaria maialis, Narcissus poeticus, Caltha palustris, Cystisus laburnum, Orchis maculata, Bilbergia, Eranthus, and Crocus were tested for seed production. Exposure to sulfuric acid ranged from three to forty-eight hours. Responses were noted for varying concentrations.

  2. Insights into the relationships of Palearctic and Nearctic lymnaeids (Mollusca : Gastropoda by rDNA ITS-2 sequencing and phylogeny of stagnicoline intermediate host species of Fasciola hepatica

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    Bargues M.D.

    2003-09-01

    Full Text Available Fascioliasis by Fasciola hepatica is the vector-borne disease presenting the widest latitudinal, longitudinal and altitudinal distribution known. F. hepatica shows a great adaptation power to new environmental conditions which is the consequence of its own capacities together with the adaptation and colonization abilities of its specific vector hosts, freshwater snails of the family Lymnaeidae. Several lymnaeid species only considered as secondary contributors to the liver fluke transmission have, however, played a very important role in the geographic expansion of this disease. Many of them belong to the so-called "stagnicoline" type group. Stagnicolines have, therefore, a very important applied interest in the Holarctic region, to which they are geographically restricted. The present knowledge on the genetics of stagnicolines and on their parasite-host interrelationships is, however, far from being sufficient. The present paper analyses the relationships between Palaearctic and Nearctic stagnicoline species on the base of the new light furnished by the results obtained in nuclear rDNA ITS-2 sequencing and corresponding phylogenetic studies of the lymnaeid taxa Lymnaea (Stagnicola occulta, L. (S. palustris palustris (topotype specimens and L.(S. p. turricula from Europe. Natural infections with F. hepatica have been reported in all of them. Surprisingly, ITS-2 length and G C content of L. occulta were similar and perfectly fitted within the respective ranges known in North American stagnicolines. Nucleotide differences and genetic distances were higher between L. occulta and the other European stagnicolines than between L. occulta and the North American ones. The ITS-2 sequence of L. p. turricula from Poland differed from the other genotypes known from turricula in Europe. The phylogenetic trees using the maximum-parsimony, distance and maximum-likelihood methods confirmed (i the inclusion of L. occulta in the branch of North American

  3. Dissecting the active site of the collagenolytic cathepsin L3 protease of the invasive stage of Fasciola hepatica.

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    Ileana Corvo

    Full Text Available A family of secreted cathepsin L proteases with differential activities is essential for host colonization and survival in the parasitic flatworm Fasciola hepatica. While the blood feeding adult secretes predominantly FheCL1, an enzyme with a strong preference for Leu at the S2 pocket of the active site, the infective stage produces FheCL3, a unique enzyme with collagenolytic activity that favours Pro at P2.Using a novel unbiased multiplex substrate profiling and mass spectrometry methodology (MSP-MS, we compared the preferences of FheCL1 and FheCL3 along the complete active site cleft and confirm that while the S2 imposes the greatest influence on substrate selectivity, preferences can be indicated on other active site subsites. Notably, we discovered that the activity of FheCL1 and FheCL3 enzymes is very different, sharing only 50% of the cleavage sites, supporting the idea of functional specialization. We generated variants of FheCL1 and FheCL3 with S2 and S3 residues by mutagenesis and evaluated their substrate specificity using positional scanning synthetic combinatorial libraries (PS-SCL. Besides the rare P2 Pro preference, FheCL3 showed a distinctive specificity at the S3 pocket, accommodating preferentially the small Gly residue. Both P2 Pro and P3 Gly preferences were strongly reduced when Trp67 of FheCL3 was replaced by Leu, rendering the enzyme incapable of digesting collagen. In contrast, the inverse Leu67Trp substitution in FheCL1 only slightly reduced its Leu preference and improved Pro acceptance in P2, but greatly increased accommodation of Gly at S3.These data reveal the significance of S2 and S3 interactions in substrate binding emphasizing the role for residue 67 in modulating both sites, providing a plausible explanation for the FheCL3 collagenolytic activity essential to host invasion. The unique specificity of FheCL3 could be exploited in the design of specific inhibitors selectively directed to specific infective stage

  4. Liver fluke (Fasciola hepatica) infection in cattle in Northern Ireland: a large-scale epidemiological investigation utilising surveillance data.

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    Byrne, Andrew W; McBride, Stewart; Lahuerta-Marin, Angela; Guelbenzu, Maria; McNair, Jim; Skuce, Robin A; McDowell, Stanley W J

    2016-04-14

    Liver fluke (Fasciola hepatica) is a widespread parasite of ruminants which can have significant economic impact on cattle production. Fluke infection status at the animal-level is captured during meat inspection of all animals processed for human consumption within Northern Ireland. These national datasets have not been analysed to assess their utility in uncovering patterns in fluke infection at animal- and herd-levels in Northern Ireland. We utilised a dataset of 1.2 million animal records from ~18,000 herds across 3 years (2011-2013) to assess animal- and herd-level apparent prevalence and risk-factors associated with fluke infection. Animal-level apparent prevalence was measured as the proportion of animals exhibiting evidence of fluke infection at slaughter; between herd-level infection prevalence was measured by binary categorisation of herds (infected or not). "Within herd" infection prevalence was measured using the proportion of animals within a herd that showed evidence of fluke infection per year (ranging from 0-100%). "Within herd" infection prevalence at the herd level was investigated using multivariable modelling. At the animal level, the proportion of animals slaughtered that exhibited evidence of infection was 21-25% amongst years. Across herds, the proportion of herds with at least one infected animal, varied between 61 and 65%. However, there was a significant sampling effect at the herd-level; all herds where at least 105 animals slaughtered over the study period exhibited evidence of fluke infection (100%). There was significant variation in terms of within-herd infection prevalence. Risk factors included herd type, long-term weather variation, geographic location (region) and the abattoir. Liver fluke apparent prevalence was high at the herd-level across years. However, there was lower prevalence at the animal level, which may indicate significant variation in the exposure to fluke infection within herds. The proportion infected within

  5. Collagenolytic activities of the major secreted cathepsin L peptidases involved in the virulence of the helminth pathogen, Fasciola hepatica.

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    Mark W Robinson

    Full Text Available BACKGROUND: The temporal expression and secretion of distinct members of a family of virulence-associated cathepsin L cysteine peptidases (FhCL correlates with the entry and migration of the helminth pathogen Fasciola hepatica in the host. Thus, infective larvae traversing the gut wall secrete cathepsin L3 (FhCL3, liver migrating juvenile parasites secrete both FhCL1 and FhCL2 while the mature bile duct parasites, which are obligate blood feeders, secrete predominantly FhCL1 but also FhCL2. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that FhCL1, FhCL2 and FhCL3 exhibit differences in their kinetic parameters towards a range of peptide substrates. Uniquely, FhCL2 and FhCL3 readily cleave substrates with Pro in the P2 position and peptide substrates mimicking the repeating Gly-Pro-Xaa motifs that occur within the primary sequence of collagen. FhCL1, FhCL2 and FhCL3 hydrolysed native type I and II collagen at neutral pH but while FhCL1 cleaved only non-collagenous (NC, non-Gly-X-Y domains FhCL2 and FhCL3 exhibited collagenase activity by cleaving at multiple sites within the α1 and α2 triple helix regions (Col domains. Molecular simulations created for FhCL1, FhCL2 and FhCL3 complexed to various seven-residue peptides supports the idea that Trp67 and Tyr67 in the S2 subsite of the active sites of FhCL3 and FhCL2, respectively, are critical to conferring the unique collagenase-like activity to these enzymes by accommodating either Gly or Pro residues at P2 in the substrate. The data also suggests that FhCL3 accommodates hydroxyproline (Hyp-Gly at P3-P2 better than FhCL2 explaining the observed greater ability of FhCL3 to digest type I and II collagens compared to FhCL2 and why these enzymes cleave at different positions within the Col domains. CONCLUSIONS/SIGNIFICANCE: These studies further our understanding of how this helminth parasite regulates peptidase expression to ensure infection, migration and establishment in host tissues.

  6. Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods.

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    Takeuchi-Storm, Nao; Denwood, Matthew; Hansen, Tina Vicky Alstrup; Halasa, Tariq; Rattenborg, Erik; Boes, Jaap; Enemark, Heidi Larsen; Thamsborg, Stig Milan

    2017-11-09

    The prevalence of bovine fasciolosis in Denmark is increasing but appropriate guidelines for control are currently lacking. In order to help develop a control strategy for liver fluke, a risk factor study of farm management factors was conducted and the utility of bulk tank milk (BTM ELISA) as a tool for diagnosis in Danish dairy cattle farms was assessed. This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola hepatica antibody levels in BTM. A case farm was defined as having a minimum of 3 incidents of liver condemnation due to liver fluke at slaughter (in any age group) during 2013, and control farms were located within 10 km of at least one case farm and had no history of liver condemnation due to liver fluke during 2011-2013. The selected farmers were interviewed over telephone about grazing and control practices, and BTM from these farms was collected and analysed by ELISA in 2014. The final complete dataset consisting of 131 case and 63 control farms was analysed using logistic regression. Heifers grazing on wet pastures, dry cows grazing on wet pastures, herd size, breed and concurrent beef cattle production were identified as risk factors associated with being classified as a case farm. With the categorised BTM ELISA result as the response variable, heifers grazing on wet pastures, dry cows grazing on wet pastures, and purchase of cows were identified as risk factors. Within the case and control groups, 74.8 and 12.7% of farms were positive for fasciolosis on BTM ELISA, respectively. The differences are likely to be related to the detection limit of the farm-level prevalence by the BTM ELISA test, time span between slaughter data and BTM, and the relatively low sensitivity of liver inspection at slaughter. Control of bovine fasciolosis in Denmark should target heifers and

  7. Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods

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    Nao Takeuchi-Storm

    2017-11-01

    Full Text Available Abstract Background The prevalence of bovine fasciolosis in Denmark is increasing but appropriate guidelines for control are currently lacking. In order to help develop a control strategy for liver fluke, a risk factor study of farm management factors was conducted and the utility of bulk tank milk (BTM ELISA as a tool for diagnosis in Danish dairy cattle farms was assessed. Methods This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013 using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola hepatica antibody levels in BTM. A case farm was defined as having a minimum of 3 incidents of liver condemnation due to liver fluke at slaughter (in any age group during 2013, and control farms were located within 10 km of at least one case farm and had no history of liver condemnation due to liver fluke during 2011–2013. The selected farmers were interviewed over telephone about grazing and control practices, and BTM from these farms was collected and analysed by ELISA in 2014. The final complete dataset consisting of 131 case and 63 control farms was analysed using logistic regression. Results Heifers grazing on wet pastures, dry cows grazing on wet pastures, herd size, breed and concurrent beef cattle production were identified as risk factors associated with being classified as a case farm. With the categorised BTM ELISA result as the response variable, heifers grazing on wet pastures, dry cows grazing on wet pastures, and purchase of cows were identified as risk factors. Within the case and control groups, 74.8 and 12.7% of farms were positive for fasciolosis on BTM ELISA, respectively. The differences are likely to be related to the detection limit of the farm-level prevalence by the BTM ELISA test, time span between slaughter data and BTM, and the relatively low sensitivity of liver inspection at slaughter. Conclusions

  8. Вплив фасціолоцидів на сперматогенез Fasciola hepatica in vitro

    OpenAIRE

    Соболта А.Г.

    2010-01-01

    They studied the impact of fasciolocides of brontel 10%, kombitrem as possible sources of cytological changes in Fasciola hepatica spermatogenous cycle cells, which is important to consider when studying the fascioles' mechanisms of developing resistance to fasciolocides antihelmintics.

  9. A growing degree-day model for determination of Fasciola hepatica infection risk in New Zealand with future predictions using climate change models.

    Science.gov (United States)

    Haydock, L A J; Pomroy, W E; Stevenson, M A; Lawrence, K E

    2016-09-15

    Infections of ruminants with Fasciola hepatica are considered to be of regional importance within New Zealand but there is very little recent information on its prevalence or severity other than anecdotal reports. Generally they are considered to be of secondary importance compared to gastrointestinal nematode infections. Utilizing data from Virtual Climate Stations (n=11491) distributed on a 5km grid around New Zealand a growing degree-day model was used to describe the risk of infection with liver fluke from 1972 to 2012 and then to apply the predictions to estimate the risk of fluke infections within New Zealand for the years 2040 and 2090. The growing degree-day model was validated against the most recent survey of infection within New Zealand in 1984. A strong positive linear relationship for 1984 between F. hepatica prevalence in lambs and infection risk (prisk values from 14 regions in New Zealand for 1972-2012 did not show any discernible change in risk of infection over this time period (p>0.05). Post-hoc comparisons indicate the risk in Westland was found to be substantially higher (prisk in 2040 and 2090 were detected although they did vary between different climate change scenarios. The highest average percentage changes in infection risk were found in regions with low initial risk values such as Canterbury and Otago; in these regions 2090 infection risk is expected to rise by an average of 186% and 184%, respectively. Despite the already high levels of infection risk in Westland, values are expected to rise by a further 76% by 2090. The model does show some areas with little change with Taranaki predicted to experience only very minor increases in infection risk with average 2040 and 2090 predicted changes of 0% and 29%, respectively. Overall, these results suggest the significance of F. hepatica in New Zealand farming systems is probably underestimated and that this risk will generally increase with global warming following climate change. Copyright

  10. Rattus norvegicus como indicador de la circulación de Capillaria hepatica y Taenia taeniaeformis en la Plaza Minorista de Medellín, Colombia

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    Biviana Andrea Duque

    2012-12-01

    Full Text Available Introducción. Rattus norvegicus cumple un papel epidemiológico en el mantenimiento y dispersión de agentes zoonóticos bacterianos, virales y parasitarios de interés en salud pública. La presencia de infección por helmintos en especies Rattus cercanas a poblaciones expuestas en condiciones ambientales propicias, puede convertirse en un factor de riesgo de transmisión. Objetivo. Reportar la frecuencia de infección con Capillaria hepatica y formas larvarias de Taenia taeniaeformis en ratas silvestres (R. norvegicus capturadas en una zona urbana de Medellín. Materiales y métodos. Se capturaron 254 ejemplares de R. norvegicus. Los hígados de 54 ejemplares que presentaron lesión hepática macroscópica durante la necropsia, fueron examinados por histopatología convencional. Resultados. La frecuencia de infección por C. hepatica fue de 20,1 % (51/254. Seis hígados fueron también positivos para larvas de T. taeniaeformis con una frecuencia de 2,4 % (6/254. Los hígados infestados con C. hepatica exhibían parásitos en el estadio adulto o juvenil y huevos ovalados conopérculos bipolares, asociados con hepatitis granulomatosa leve a moderada multifocal y acompañada por infiltrado leucocitario. Se observaron lesiones granulomatosas en resolución y fibrosis residual o calcificada que contenía huevos. Donde se encontraron cisticercos de T. taeniaeformis, el hallazgo más frecuente fueron quistes hepáticos que contenían larvas, y lesiones inflamatorias y fibróticas. Conclusión. Estos resultados indican que helmintos de potencial zoonótico circulan en R. norvegicus de ambientes urbanos. Debe investigarse la verdadera distribución de estos parásitos, para determinar el riesgo potencial que corren las poblaciones animales y humanas expuestas a adquirir este tipo de infecciones.   doi: http://dx.doi.org/10.7705/biomedica.v32i4.442

  11. DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis

    Science.gov (United States)

    Gayo, Valeria; Sanchis, Jaime; Artigas, Patricio; Khoubbane, Messaoud; Birriel, Soledad; Mas-Coma, Santiago

    2017-01-01

    Background Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. A study including DNA marker sequencing of fasciolids and lymnaeids, an experimental study of the life cycle in Uruguay, and a review of human fascioliasis in Uruguay, are performed. Methodology/Principal findings The characterization of Fasciola hepatica from cattle and horses of Uruguay included the complete sequences of the ribosomal DNA ITS-2 and ITS-1 and mitochondrial DNA cox1 and nad1. ITS-2, ITS-1, partial cox1 and rDNA 16S gene of mtDNA were used for lymnaeids. Results indicated that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. The life cycle and transmission features of F. hepatica by L. neotropica of Uruguay were studied under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. On this baseline, we reviewed the 95 human fascioliasis cases reported in Uruguay and analyzed the risk of human infection in front of future climate change estimations. Conclusions/Significance The correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock into Uruguay allowed to understand the molecular diversity detected. Although Uruguayan L. neotropica is a highly efficient vector, its transmission capacity is markedly lower than that of Bolivian G. truncatula. This allows to understand the transmission and

  12. Casos autóctones de Fasciola hepatica na região de Presidente Prudente, São Paulo, Brasil

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    Tostes Raimundo Alberto

    2004-01-01

    Full Text Available O presente trabalho descreve, a partir da condenação de fígado de bovinos em matadouro, a ocorrência de fasciolose em uma propriedade na região de Presidente Prudente, São Paulo, com a confirmação de ovos de Fasciola hepatica em exame coproparasitológico e a presença de caramujos do gênero Lymnaea. Observou-se que a propriedade ofereceu condições favoráveis ao desenvolvimento do hospedeiro intermediário do parasito e ao surgimento de casos autóctones na região. A verificação desses casos deve servir de alerta para as potenciais perdas econômicas advindas desse tipo de parasitismo.

  13. Assessment of the FasciMol-ELISA in the detection of the trematode Fasciola hepatica in field-collected Galba cubensis: a novel tool for the malacological survey of fasciolosis transmission.

    Science.gov (United States)

    Alba, Annia; Vázquez, Antonio A; Sánchez, Jorge; Fraga, Jorge; Hernández, Hilda; Martínez, Elizabeth; Marcet, Ricardo; Figueredo, Mabel; Sarracent, Jorge

    2016-01-16

    Fasciolosis is one of the food-borne neglected trematodioses that has reemerged as a human disease while its effects on domestic animal health remains of significant economic consideration. Being snail-borne disease, the accurate and time-saving epidemiological surveillance of the transmission foci where infected lymnaeid snails occur could be essential to effectively focus or redirect control strategies. For this purpose, the first monoclonal antibody-based immunoenzymatic assay to detect Fasciola hepatica-infected snails (FasciMol-ELISA) was recently developed and showed a high sensitivity and specificity when tested in an experimental F. hepatica - Galba cubensis system. Here, we surveyed populations of G. cubensis occurring in western Cuba for the assessment of the FasciMol-ELISA in determining natural F. hepatica infection in this intermediate host. A multiplex PCR, previously developed to detect F. hepatica in G. cubensis, was used for sample classification. Snail dissection method was also employed as screening technique. A Χ(2) test and a Kappa index were calculated to evaluate the positivity and the level of agreement between the FasciMol-ELISA and the snail dissection methods with the multiplex PCR, respectively. Galba cubensis was found in nine out of 12 sampled localities of which four were positive for F. hepatica infection as detected by both immunoenzymatic and PCR-based assays. The overall prevalence was higher than the natural infection rates previously reported for Cuban G. cubensis (range from 4.1 to 7.42% depending on the screening method). No significant differences were found between FasciMol-ELISA and multiplex PCR when determining parasite positivity (Χ(2) = 6.283; P = 0.0981) whereas an excellent agreement was also noted (Kappa = 0.8224). Our results demonstrate the importance of malacological surveys in assessing parasite transmission risk and constitute an alert on the need of accurate measures to control fasciolosis in

  14. Fasciola hepatica: a light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy.

    Science.gov (United States)

    Hanna, R E B; Moffett, D; Forster, F I; Trudgett, A G; Brennan, G P; Fairweather, I

    2016-05-15

    The ultrastructure of the ovary of Fasciola hepatica collected from field-infected sheep, was compared with that of flukes from laboratory-infected rats harbouring the Oberon or the Cullompton fluke isolate. At the periphery of the ovarian tubules, in all flukes, interstitial tissue was identified that appears to provide physical support and facilitate the metabolism of the germinal-line cells. Oogonia undergo mitotic division to maintain the cell population and to produce oocytes. Early oocytes feature conspicuous synaptonemal complexes in the nucleoplasm, and these become less evident as the oocytes grow in size, move towards the core of the ovarian tubule, and synthesise osmiophilic bodies. The latter may represent cortical granules, and serve to block polyspermy. The identity of the synaptonemal complexes was confirmed by immunocytochemical labelling of synaptonemal proteins. The occurrence of synaptonemal complexes in the oocytes of all fluke types examined indicates that pairing of bivalent chromosomes, with the potential for genetic recombination and chiasmata formation, is a feature of the triploid aspermic parthenogenetic Cullompton flukes, as well as of the wild-type out-breeding field-derived and Oberon isolate flukes. In oocytes within shelled eggs in the proximal uterus of all flukes, condensed chromosomes align at meiotic metaphase plates. Following the reduction division, two equal pronuclei appear in each oocyte in the distal uterus. On the basis of these observations, a mechanism of facultative parthenogenesis for F. hepatica is proposed that accommodates the survival and clonal expansion of triploid aspermic isolates. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Extracellular Vesicles From the Helminth Fasciola hepatica Prevent DSS-Induced Acute Ulcerative Colitis in a T-Lymphocyte Independent Mode

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    Javier Roig

    2018-05-01

    Full Text Available The complexity of the pathogenesis of inflammatory bowel disease (ulcerative colitis and Crohn’s disease has led to the quest of empirically drug therapies, combining immunosuppressant agents, biological therapy and modulators of the microbiota. Helminth parasites have been proposed as an alternative treatment of these diseases based on the hygiene hypothesis, but ethical and medical problems arise. Recent reports have proved the utility of parasite materials, mainly excretory/secretory products as therapeutic agents. The identification of extracellular vesicles on those secreted products opens a new field of investigation, since they exert potent immunomodulating effects. To assess the effect of extracellular vesicles produced by helminth parasites to treat ulcerative colitis, we have analyzed whether extracellular vesicles produced by the parasitic helminth Fasciola hepatica can prevent colitis induced by chemical agents in a mouse model. Adult parasites were cultured in vitro and secreted extracellular vesicles were purified and used for immunizing both wild type C57BL/6 and RAG1-/- mice. Control and immunized mice groups were treated with dextran sulfate sodium 7 days after last immunization to promote experimental colitis. The severity of colitis was assessed by disease activity index and histopathological scores. Mucosal cytokine expression was evaluated by ELISA. The activation of NF-kB, COX-2, and MAPK were evaluated by immunoblotting. Administration of extracellular vesicles from F. hepatica ameliorates the pathological symptoms reducing the amount of pro-inflammatory cytokines and interfering with both MAPK and NF-kB pathways. Interestingly, the observed effects do not seem to be mediated by T-cells. Our results indicate that extracellular vesicles from parasitic helminths can modulate immune responses in dextran sulfate sodium (DSS-induced colitis, exerting a protective effect that should be mediated by other cells distinct from B

  16. Evaluation of local immune response to Fasciola hepatica experimental infection in the liver and hepatic lymph nodes of goats immunized with Sm14 vaccine antigen

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    Ricardo E Mendes

    2010-08-01

    Full Text Available Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14 was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic morphometric observation. The nature of the local immune response was assessed by examining the distribution of CD2+, CD4+, CD8+ and γ´+ T lymphocytes along with IgG+, IL-4+ and IFN-γ+ cells in the liver and hepatic lymph nodes (HLN. The goats used consisted of group 1 (unimmunized and uninfected, group 2 [infected control - immunized with Quillaia A (Quil A] and group 3 (immunized with rSm14 in Quil A and infected, each containing seven animals. Immunization with rSm14 in Quil A adjuvant induced a reduction in gross hepatic lesions of 56.6% (p < 0.001 and reduced hepatic and HLN infiltration of CD2+, CD4+, CD8+ and γ´+ T lymphocytes as well as IL-4+ and IFN-γ+ cells (p < 0.05. This is the first report of caprine immunization against F. hepatica using a complete rSm14 molecule derived from S. mansoni. Immunization reduced hepatic damage and local inflammatory infiltration into the liver and HLN. However, considering that Quil A is not the preferential/first choice adjuvant for Sm14 immunization, further studies will be undertaken using the monophosphoryl lipid A-based family of adjuvants during clinical trials to facilitate anti-Fasciolavaccine development.

  17. PREVALENCIA DE Fasciola hepatica Y PÉRDIDAS ECONÓMICAS ASOCIADAS AL DECOMISO DE HÍGADOS EN TRES MATADEROS DE CLASE A DE COSTA RICA

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    Diana Rojas

    2016-01-01

    Full Text Available Para estimar la prevalencia anual y men - sual de Fasciola hepatica y las pérdidas econó - micas anuales asociadas al decomiso de hígados por presencia del parásito, se analizaron los registros del Ministerio de Agricultura y Gana - dería de Costa Rica, Dirección de Inocuidad de Productos de Origen Animal (DIPOA, para el 2014, provenientes de 3 mataderos clase A localizados en el área metropolitana de Costa Rica; en estos mataderos se sacrificaron 249 108 reses, de las cuales 4547 hígados fueron decomi - sados por presencia de F. hepatica en el órgano. Las mayores prevalencias, entre 2,33 y 2,55%, se presentaron en enero, febrero y marzo, y las menores, entre 1,32 y 1,56%, durante agosto, setiembre y octubre. La prevalencia anual según estos registros fue 1,83% (IC 95%: 1,77-1,88. Las pérdidas económicas asociadas al decomiso de hígados fueron de 36.379.000 CRC, equivalentes a 67.438 USD. Se resalta el perjuicio económico de este parásito a nivel nacional y la utilidad del decomiso y registro de vísceras afectadas en los establecimientos de sacrificio de bovinos, como herramienta diagnóstica para la vigilancia epidemiológica, disponible para conocer sobre el estado de esta parasitosis. Como alternativas de control y prevención de la enfermedad, se plantea considerar las condiciones específicas de las regiones afectadas y así controlar esta parasitosis con un manejo integral, que involucre la fuente de alimento, desparasitación regular del ganado, con registro y monitoreo farmacológico de las drogas utilizadas, y saneamiento ambiental en fincas afectadas.

  18. "Purification and evaluation of somatic, excretory-secretory and Cysteine proteinase antigens of Fasciola Hepatica using IgG-ELISA in diagnosing Fascioliasis "

    Directory of Open Access Journals (Sweden)

    "Rokni MB

    2001-08-01

    Full Text Available Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man and animals, in the world and Iran, particularly in nortern parts. The economical losses in domestic animals are considerable. In the recent decade there were two major outbreaks of human fasciolosis in the Caspian region, northern part of Iran with 7000-10000 infected cases. Sicne it is impossible to diagnose fasciolosis in acute phase using coprological methods and even in chronic phases its sensitivity is low, evaluating and establishing a reliable and cost-effetive test is indispensable and notewortly.In the present survey, we produced and examined the sensitivity and specificity of liver fluke homogenate (LFH , excretory-secetory (ES and cysteine proteinase (CP antigens of F. hepatica using IgG-ELISA test. A 25-27 kilo Dalton coomassie blue-stained band was observed and using of specific inhibitors indicated that this antigen belongs to the class of cysteine proteinase. The sensitivity of LFH, ES and CP antigen in IgG-ELISa was 100% for each, while their specificity was 97.8%, 98.8% and 98.8% respectively. There was a significant difference in mean OD values between cases of proven fasciolosis and other true negative cases, including healthy control individuals and patients with other parasitic diseases.This present report is the first to demonstrate the purification and evaluation of F. hepatica cysteine proteinase antigen by IgG-ELISA test for the diagnosis of fasciolosis in Iran. In conclusion, the IgG-ELISa using ES and CP show high sensitivity and specificity and would be a valuable tool to diagnose human fasciolosis in Iran, particularly in endemic areas.

  19. CONTROL DE FASCIOLA HEPATICA EN EL AGUA DE CONSUMO ANIMAL A TRAVÉS DE FILTRACIÓN RÁPIDA Y LENTA

    Directory of Open Access Journals (Sweden)

    Carolina González Morales

    Full Text Available La fasciolosis bovina es una zoonosis causada por Fasciola hepatica, parásito que se adquiere al consumir agua o vegetales contaminados con el digeneo. Esta enfermedad ocasiona pérdidas económicas importantes en las regiones lecheras de Antioquia. En este trabajo, y con el objetivo de remover los huevos del parásito presentes en el agua, se diseñaron y construyeron dos filtros empleando como lecho filtrante arena industrial (T.E entre 0,45 y 0,55 mm, con los cuales se evaluó un sistema de filtración rápida operado a velocidades de 5 y 8 m/h (altura del lecho 60 cm y un sistema de filtración lenta a una velocidad de 1,46 m/h (alturas del lecho 30 y 40 cm. Se determinó la variación del caudal y la turbiedad del efluente durante la carrera de filtración y la eficiencia de remoción de los huevos del parásito. Los filtros operando bajo las características descritas, fueron 100% eficientes para remover los huevos de F. hepatica presentes en el agua. Los porcentajes de remoción de turbiedad fueron del 85,5 y 79,4% para filtros trabajando a velocidades de 5 m/h y 8 m/h, respectivamente. Los ensayos realizados para ambas alturas del lecho filtrante (30 y 40 cm operando con la velocidad de 1,46 m/h, mostraron una remoción promedio de turbiedad del 80,4 y 76,6%, respectivamente.

  20. Comparação de kits ELISA® comerciais para anticorpos no soro e leite com um teste coproparasitológico em bovinos naturalmente infectados por Fasciola hepatica Comparison of comercial® ELISA kits for antibodies in serum and milk with a fecal test in cattle naturally infected with Fasciola hepatica

    Directory of Open Access Journals (Sweden)

    Cíntia das C. Bernardo

    2013-01-01

    Full Text Available A fasciolose é uma enfermidade causada por um trematoda que acomete o fígado principalmente de ruminantes domésticos, podendo parasitar o homem e seu diagnóstico é realizado rotineiramente por exames coproparasitológicos. O objetivo do presente estudo foi comparar kits comerciais de ELISA para anticorpos no soro e leite com um teste coproprarasitológico em bovinos naturalmente infectados por Fasciola hepatica. Foram coletadas amostras de fezes (92 sangue (92 e leite (43 de bovinos provenientes de propriedades de gado leiteiro do município de Jerônimo Monteiro, sul do Estado do Espírito Santo. As amostras de fezes coletadas foram processadas pela técnica de sedimentação fecal para ovos de F. hepatica, utilizada como padrão ouro para as análises. Amostras de sangue e de leite foram processadas segundo a orientação do fabricante dos respectivos Kits ELISA comerciais testados. Utilizou-se o c² de McNemar para comparação estatística e calcularam-se a sensibilidade e especificidade, valores preditivos e kappa. Os resultados obtidos mostraram que as frequências de positividade pelo uso dos kits ELISA comerciais de soro e de leite diferiram significativamente (pThe fascioliasis is a disease caused by a trematode that affects the liver mainly of domestic ruminants and can also parasite man; its diagnosis is routinely done by coprological methods. The aim of this study was to compare commercial ELISA kits for antibodies in serum and milk with a coprological test in cattle naturally infected by Fasciola hepatica. We collected fecal, blood and milk samples from cattle in the municipality of Jerônimo Monteiro, southern Espírito Santo state. The fecal samples were processed by the fecal egg sedimentation for F. hepatica, which is used as a gold standard for analyzis. Blood (92 and milk (43 samples were processed according to the manufacturer instructions of the respective commercial ELISA kits tested. We used the McNemar chi-square for

  1. INFESTATION BY FASCIOLA HEPATICA

    African Journals Online (AJOL)

    It is the purpose of this paper to draw attention to the ... dissolves and frees a young fluke; this bores through the ... bile it becomes an adult hermaphroditic parasite and lays .... man for even longer periods (c. sinensis has a life-span of.

  2. Fasciola hepatica reinfection potentiates a mixed Th1/Th2/Th17/Treg response and correlates with the clinical phenotypes of anemia

    Science.gov (United States)

    Perez-Crespo, Ignacio; Chillón-Marinas, Carlos; Khoubbane, Messaoud; Quesada, Carla; Reguera-Gomez, Marta; Mas-Coma, Santiago; Fresno, Manuel; Gironès, Núria

    2017-01-01

    Background Fascioliasis is a severe zoonotic disease of worldwide extension caused by liver flukes. In human fascioliasis hyperendemic areas, reinfection and chronicity are the norm and anemia is the main sign. Herein, the profile of the Th1/Th2/Th17/Treg expression levels is analyzed after reinfection, correlating them with their corresponding hematological biomarkers of morbidity. Methodology/Principal findings The experimental design reproduces the usual reinfection/chronicity conditions in human fascioliasis endemic areas and included Fasciola hepatica primo-infected Wistar rats (PI) and rats reinfected at 8 weeks (R8), and at 12 weeks (R12), and negative control rats. In a cross-sectional study, the expression of the genes associated with Th1 (Ifng, Il12a, Il12b, Nos2), Th2 (Il4, Arg1), Treg (Foxp3, Il10, Tgfb, Ebi3), and Th17 (Il17) in the spleen and thymus was analyzed. After 20 weeks of primary infection, PI did not present significant changes in the expression of those genes when compared to non-infected rats (NI), but an increase of Il4, Arg1 and Ifng mRNA in the spleen was observed in R12, suggesting the existence of an active mixed Th1/Th2 systemic immune response in reinfection. Foxp3, Il10, Tgfb and Ebi3 levels increased in the spleen in R12 when compared to NI and PI, indicating that the Treg gene expression levels are potentiated in chronic phase reinfection. Il17 gene expression levels in R12 in the spleen increased when compared to NI, PI and R8. Gene expression levels of Il10 in the thymus increased when compared to NI and PI in R12. Ifng expression levels in the thymus increased in all reinfected rats, but not in PI. The clinical phenotype was determined by the fluke burden, the rat body weight and the hemogram. Multivariate mathematical models were built to describe the Th1/Th2/Th17/Treg expression levels and the clinical phenotype. In reinfection, two phenotypic patterns were detected: i) one which includes only increased splenic Ifng

  3. Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis.

    Directory of Open Access Journals (Sweden)

    Bruno Gottstein

    2014-06-01

    Full Text Available Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2 and cathepsin L-1 (recCL1, were assessed individually and in combination in enzyme-linked immunosorbent assays (ELISA for the specific serodiagnosis of human fasciolosis in areas of low endemicity as encountered in Central Europe. Antibody detection was conducted using ProteinA/ProteinG (PAG conjugated to alkaline phosphatase. Test characteristics as well as agreement with results from an ELISA using excretory-secretory products (FhES from adult stage liver flukes was assessed by receiver operator characteristic (ROC analysis, specificity, sensitivity, Youdens J and overall accuracy. Cross-reactivity was assessed using three different groups of serum samples from healthy individuals (n=20, patients with other parasitic infections (n=87 and patients with malignancies (n=121. The best combined diagnostic results for recombinant antigens were obtained using the recSAP2-ELISA (87% sensitivity, 99% specificity and 97% overall accuracy employing the threshold (cut-off to discriminate between positive and negative reactions that maximized Youdens J. The findings showed that recSAP2-ELISA can be used for the routine serodiagnosis of chronic fasciolosis in clinical laboratories; the use of the PAG-conjugate offers the opportunity to employ, for example, rabbit hyperimmune serum for the standardization of positive controls.

  4. The population density of Lymnaea columella (Say, 1817) (Mollusca, Lymnaeidae) an intermediate host of Fasciola hepatica (Linnaeus, 1758), in the Caparaó microregion, ES, Brazil.

    Science.gov (United States)

    D'Almeida, S C G; Freitas, D F; Carneiro, M B; Camargo, P F; Azevedo, J C; Martins, I V F

    2016-06-01

    The aim of this study was to monitor the population density of Lymnaea columella, an intermediate host of Fasciola hepatica, in various aquatic habitats and in drinking water in the area of the Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, on Caparaó Microregion, municipality of Alegre, state of Espírito Santo, Brazil. Monthly samplings were performed at certain points between drainage areas and drinking water in cattle and goat production systems during the years 2010 to 2013. The mean temperature, precipitation and the frequency of samples of L. columella were analysed graphically according the monthly average during the study period. A total of 2,038 molluscs were collected, 1558 of which were L. columella, that predominated in all sampled points. The highest average of specimens observed for L. columella was in the years 2010 and 2013 (51.0), and occurred decreased in 2011 (19.8). The temperature and precipitation averaged is 23.7 °C and 141 mm/year, respectively. Rainfall peak occurred in March (2011, 2013) and November (2012), during these periods the population of L. columella growth. There was no significant difference in the relationship between the specimens observed with seasons (dry-wet), thus the population of L. columella remained stable and can be found throughout the year.

  5. Characterization of Fasciola samples by ITS of rDNA sequences revealed the existence of Fasciola hepatica and Fasciola gigantica in Yunnan Province, China.

    Science.gov (United States)

    Shu, Fan-Fan; Lv, Rui-Qing; Zhang, Yi-Fang; Duan, Gang; Wu, Ding-Yu; Li, Bi-Feng; Yang, Jian-Fa; Zou, Feng-Cai

    2012-08-01

    On mainland China, liver flukes of Fasciola spp. (Digenea: Fasciolidae) can cause serious acute and chronic morbidity in numerous species of mammals such as sheep, goats, cattle, and humans. The objective of the present study was to examine the taxonomic identity of Fasciola species in Yunnan province by sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA (rDNA). The ITS rDNA was amplified from 10 samples representing Fasciola species in cattle from 2 geographical locations in Yunnan Province, by polymerase chain reaction (PCR), and the products were sequenced directly. The lengths of the ITS-1 and ITS-2 sequences were 422 and 361-362 base pairs, respectively, for all samples sequenced. Using ITS sequences, 2 Fasciola species were revealed, namely Fasciola hepatica and Fasciola gigantica. This is the first demonstration of F. gigantica in cattle in Yunnan Province, China using a molecular approach; our findings have implications for studying the population genetic characterization of the Chinese Fasciola species and for the prevention and control of Fasciola spp. in this province.

  6. Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool

    Science.gov (United States)

    Cabada, Miguel M.; Malaga, Jose L.; Castellanos-Gonzalez, Alejandro; Bagwell, Kelli A.; Naeger, Patrick A.; Rogers, Hayley K.; Maharsi, Safa; Mbaka, Maryann; White, A. Clinton

    2017-01-01

    Fasciola hepatica is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)–based methods offer high sensitivity and specificity but require expensive technology. However, the recombinase polymerase amplification (RPA) is an efficient isothermal method that eliminates the need for a thermal cycler and has a high deployment potential to resource-limited settings. We report on the characterization of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites. In addition, RPA and PCR were able to detect 47% and 26% of infections not detected by microscopy, respectively. The RPA adapted to a lateral flow platform was more sensitive than gel-based detection of the reaction products. In conclusion, the Fasciola RPA is a highly sensitive and specific test to diagnose chronic infection using stool samples. The Fasciola RPA lateral flow has the potential for deployment to endemic areas after further characterization. PMID:27821691

  7. Evidence for high genetic diversity of NAD1 and COX1 mitochondrial haplotypes among triclabendazole resistant and susceptible populations and field isolates of Fasciola hepatica (liver fluke) in Australia.

    Science.gov (United States)

    Elliott, T; Muller, A; Brockwell, Y; Murphy, N; Grillo, V; Toet, H M; Anderson, G; Sangster, N; Spithill, T W

    2014-02-24

    In recent years, the global incidence of Fasciola hepatica (liver fluke) infections exhibiting resistance to triclabendazole (TCBZ) has increased, resulting in increased economic losses for livestock producers and threatening future control. The development of TCBZ resistance and the worldwide discovery of F. hepatica population diversity has emphasized the need to further understand the genetic structure of drug susceptible and resistant Fasciola populations within Australia. In this study, the genetic diversity of liver flukes was estimated by sequencing mitochondrial DNA (mtDNA) encoding the NAD1 (530 bp) and COX1 (420 bp) genes of 208 liver flukes (F. hepatica) collected from three populations: field isolates obtained from abattoirs from New South Wales (NSW) and Victoria (Vic); three TCBZ-resistant fluke populations from NSW and Victoria; and the well-established TCBZ-susceptible Sunny Corner laboratory isolate. Overall nucleotide diversity for all flukes analysed of 0.00516 and 0.00336 was estimated for the NAD1 and COX1 genes respectively. Eighteen distinct haplotypes were established for the NAD1 gene and six haplotypes for the COX1 gene, resulting in haplotype diversity levels of 0.832 and 0.482, respectively. One field isolate showed a similar low level of haplotype diversity as seen in the Sunny Corner laboratory isolate. Analysis of TCBZ-resistant infrapopulations from 3 individual cattle grazing one property revealed considerable sequence parasite diversity between cattle. Analysis of parasite TCBZ-resistant infrapopulations from sheep and cattle revealed haplotypes unique to each host, but no significant difference between parasite populations. Fst analysis of fluke populations revealed little differentiation between the resistant and field populations. This study has revealed a high level of diversity in field and drug resistant flukes in South-Eastern Australia. Copyright © 2013 Elsevier B.V. All rights reserved.

  8. T2-weighted MR imaging of liver lesions: a prospective evaluation comparing turbo spin-echo, breath-hold turbo spin-echo and half-Fourier turbo spin-echo (HASTE) sequences; Estudio de lesiones hepaticas con imagenes de resonancia magnetica potenciadas en T2: evaluacion prospectiva comparando secuencias turbo eco del espin, turbo eco del espin con respiracion sostenida y half-Fourier turbo eco del espin (HASTE)

    Energy Technology Data Exchange (ETDEWEB)

    Martin, J.; Villajos, M.; Oses, M. J.; Veintemillas, M.; Rue, M.; Puig, J.; Sentis, M. [Fundacion Parc Tauli. Sabadell (Spain)

    2000-07-01

    To compare turbo spin-echo (TSE), breath-hold TSE and half-Fourier acquisition single-shot turbo spin-echo (HASTE) sequences quantitatively and qualitatively in T2-weighted images of liver lesions. The authors evaluated prospectively 89 liver lesions in 73 patients using a 1.0-T magnetic resonance system to compare TSE, breath-hold TSE and HASTE sequences. The quantitative parameters were: lesion-to-liver contrast and lesion-to-liver contrast-to-noise ratio. The qualitative analysis was performed by two observers in consensus who examined four parameters: respiratory artifacts, lesion edge definition, intrahepatic vessel definition and image quality. Repeated measures analysis of variance was utilized to compare the quantitative variables and Friedman's nonparametric test for the qualitative parameters. In quantitative terms, the lesion-to-liver contrast was similar in TSE and breath-hold TSE sequences (2.45{+-}1.44 versus 2.60{+-}1.66), both of which were significantly better than the HASTE sequence (1.12{+-}0.72; p<0.001). The lesion-to-liver contrast-to-noise ratio was significantly higher in the TSE sequence (62.60{+-}46.40 versus 40.22{+-}25.35 versus 50.90{+-}32.10 for TSE, breath-hold TSE and HASTE sequences, respectively; p<0.001). In the qualitative comparisons, the HASTE sequence was significantly better than the TSE and breath-hold TSE sequences (p<0.001) in terms of artifacts and definition of lesion edge and intrahepatic vessels. Image quality was also significantly greater in the HASTE sequence (p<0.001). In quantitative terms, the TSE sequence is better than the breath-hold TSE and HASTE sequences, but there are no movement artifacts in the HASTE sequence, which is also significantly superior to TSE and breath-hold TSE sequences in qualitative terms and, thus, can be employed for T2-weighted images in liver studies. (Author) 17 refs.

  9. Immunization against Capillaria hepatica: The effects of primary infections, X-irradiated stages, non-embryonated eggs and soluble egg extracts

    Energy Technology Data Exchange (ETDEWEB)

    Zahner, H.; Schmidt, H.; Laemmler, G.; Geyer, E.

    1980-01-01

    The worm reproductivity was significantly suppressed in a sublethal challenge infection given 11 days after a primary infection of Mastomys natalensis with 50, 150, 400, and 800 eggs per animal. The administration of 600 X-irradiated (2.2 Krd) embryonated eggs 36 days before challenge as well as an intraperitoneal injection of non-embryonated eggs 12, 10, 8, 6, 4, and 2 days before challenge also reduced significantly the egg production of a weak (50 eggs/animal) infection. No effect was observed on a moderate challenge (300 eggs/animal). The effect was not markedly enhanced by the repeated administration of X-irradiated eggs or by the combination of X-irradiated infective eggs and non-embryonated eggs. Immunization of mice with soluble egg extracts resulted in significant reduction of egg production determined 60 days after challenge. Two hundred and thirty eggs of C. hepatica/g bodyweight proved to be a lethal infection dose for M. natalensis. The animals died between 20 and 35 days after infection. After single infections with 50, 150, 400, or 800 eggs per animal the mortality of Mastomys challenged 36 or 52 days later was reduced to 0-30%. Using X-irradiated embryonated eggs for immunization only repeated administration led to protection in 70 to 80%, of the animals. About 40% of the animals could be protected by the intraperitoneal injection of non-embryonated eggs. If death occurred it was delayed. The combination of X-irradiated stages and eggs did not enhance the protection.

  10. X-ray sensititvity of embryonated Capillaria hepatica eggs and serum-GLDH activities and antibody titres in Mastomys natalensis infected with untreated or irradiated eggs

    Energy Technology Data Exchange (ETDEWEB)

    Zahner, H.; Schmidt, H.; Laemmler, G.; Geyer, E.

    1981-01-01

    X-ray irradiation of embryonated Capillaria hepatica eggs using 0.5, 1, or 2 Krd resulted in a progressive decrease of egg production of the female nematodes which had developed from irradiated first stage larvae in Mastomys natalensis. Egg production did not occur after irradiation with 3, 4, 5, 10, 30, 50, or 70 Krd. The capacity of the parasites to invade the liver was not influenced. Infection of M. natalensis using unirradiated eggs was followed by an increase of serum-GLDH-activities between days 6 and 8 post infection reaching maximum values in this period of infection. Furthermore high values have been determined after the beginning of patency. Increased activity persisted up to the end of the experiment on day 36 post infection. After infection with eggs which had received 2.2 or 5 Krd the increase of serum-GLDH-activities was decreased and occurred later in the course of infection using 5 Krd irradiated eggs. Antibodies could be demonstrated as early as one week after infection with unirradiated eggs. Employing the indirect haemagglutination test, using an aqueous extract from non-embryonated eggs as antigen, maximum titres occurred at the beginning of patency. After a nearly plateau-like course titres began to drop about 7 weeks p.i., i.e. about the end of egg production by the female worms, but antibodies were still detectable 17 weeks p.i. (end of the observation period). After infection with eggs which had received 2.2 or 5 Krd antibody development was delayed. Maximum titres were somewhat (2.2 Krd) or markedly (5 Krd) lower. Thereafter titres dropped to values comparable to those of uninfected M. natalensis. The results are compared with published reports on the pathohistology of capillariasis.

  11. Transmission dynamics of Fasciola hepatica in the Plateau Region of Mexico. Effect of weather and treatment of mammals under current farm management.

    Science.gov (United States)

    Cruz-Mendoza, Irene; Quiroz-Romero, Héctor; Correa, Dolores; Gómez-Espinoza, Guillermo

    2011-01-10

    The aim of the present work was to study the dynamics of Fasciola hepatica natural infection in ovines, caprines, bovines and two mollusc species, Lymnaea (Fossaria) humilis and Lymnaea (F.) bulimoides, from 2004 to mid 2007 under normal farm management conditions, and the relation to climate changes. The study was performed in a research centre in the plateau of Mexico. Temperature and rainfall were registered every month, as well as the number and intensity of infection in livestock and molluscs, as determined by coprology and direct observation/cercariae release, respectively. The first two years mammals were treated with clorsulon/ivermectin because the animals were harbouring concomitant intestinal nematode infections and this was the available drug combination. During the second period treatment was with triclabendazole. The temperature ranged from around cero to 30 °C, except during September 2005 to January 2006, when a cold climate prevailed. The rainfall augmented every year in July-August, and slightly in April, 2006. Lymneid snails appeared during or immediately after the rainfall peaks of 2004 and 2006, while few L. humilis and no L. bulimoides were present during the same period of 2005, probably because it was cold. A total of 15564 cercariae were released from molluscs during the wet time of 2004, 76 during 2005 and 368 in 2006. Several peaks of infection in mammals were observed, most occurring up to 4 months after the snails had disappeared. As expected, the weather had strong impact on snails and then on livestock infection. Also, treatment given to livestock was related to reduced cercarieae release five months later. Therefore, the combination of treatment and inspection of snails in the biotopes where the livestock graze may facilitate control of fasciolosis under current farm management. Copyright © 2010 Elsevier B.V. All rights reserved.

  12. X-ray sensititvity of embryonated Capillaria hepatica eggs and serum-GLDH activities and antibody titres in Mastomys natalensis infected with untreated or irradiated eggs

    International Nuclear Information System (INIS)

    Zahner, H.; Schmidt, H.; Laemmler, G.; Geyer, E.

    1981-01-01

    X-ray irradiation of embryonated Capillaria hepatica eggs using 0.5, 1, or 2 Krd resulted in a progressive decrease of egg production of the female nematodes which had developed from irradiated first stage larvae in Mastomys natalensis. Egg production did not occur after irradiation with 3, 4, 5, 10, 30, 50, or 70 Krd. The capacity of the parasites to invade the liver was not influenced. Infection of M. natalensis using unirradiated eggs was followed by an increase of serum-GLDH-activities between days 6 and 8 post infection reaching maximum values in this period of infection. Furthermore high values have been determined after the beginning of patency. Increased activity persisted up to the end of the experiment on day 36 post infection. After infection with eggs which had received 2.2 or 5 Krd the increase of serum-GLDH-activities was decreased and occurred later in the course of infection using 5 Krd irradiated eggs. Antibodies could be demonstrated as early as one week after infection with unirradiated eggs. Employing the indirect haemagglutination test, using an aqueous extract from non-embryonated eggs as antigen, maximum titres occurred at the beginning of patency. After a nearly plateau-like course titres began to drop about 7 weeks p.i., i.e. about the end of egg production by the female worms, but antibodies were still detectable 17 weeks p.i. (end of the observation period). After infection with eggs which had received 2.2 or 5 Krd antibody development was delayed. Maximum titres were somewhat (2.2 Krd) or markedly (5 Krd) lower. Thereafter titres dropped to values comparable to those of uninfected M. natalensis. The results are compared with published reports on the pathohistology of capillariasis. (orig.) [de

  13. Serological and biochemical follow-up in cattle naturally infected with Fasciola hepatica, and comparison with a climate model for predicting risks of fasciolosis.

    Science.gov (United States)

    Bossaert, K; Lonneux, J F; Godeau, J M; Peeters, J; Losson, B

    1999-01-01

    Several biological parameters were measured in 31 heifers naturally infected with Fasciola hepatica during one grazing season in the Belgian Ardennes. A forecast model based on daily temperature used to assess the risk of fasciolosis was fitted to this assay. Cattle were turned out to two pastures. Each pasture was divided into two plots: one was treated with calcium cyanamide and the other was left untreated. The Lymnaea truncatula snails were counted on three different occasions. The results indicated a poor molluscicide efficiency. Body weight gains, anti-Fasciola antibody levels, faecal egg counts, levels of sorbitol dehydrogenase (SDH) and gamma-glutamyl transferase (gamma GT), packed cell volumes, white blood cells and differential leucocyte counts were determined monthly. No statistically significant difference was observed between animals from the two plots regardless of the recorded data. No correlation was found between body weight gains and other biological data. The sampling date had a significant effect on the antibody responses within a same group, and on the enzymatic levels for all groups combined. The forecast results were consistent with the recorded data. Temperature was a major bioclimatic constraint on the transmission of life cycle, and risk of infection occurred mainly in late spring (May/June) and in early September. Current results might be used to issue advice on the need for flukicide treatment of cattle. The indicators of the infection considered alone were useless and it is concluded that herd diagnosis of fasciolosis may rely on the rise of specific antibody levels, possibly associated with an increase in hepatic enzyme activities.

  14. Evaluación de una prueba de ELISA con antígeno metabólico de Fasciola hepatica para el diagnóstico de fasciolosis humana en Cajamarca, Perú Evaluation of an ELISA test with Fasciola hepatica metabolic antigen for diagnosis of human fascioliasis in Cajamarca, Peru

    Directory of Open Access Journals (Sweden)

    Hernán Cornejo

    2010-12-01

    Full Text Available Se obtuvo el antígeno metabólico (antígeno excreción - secreción de Fasciola hepatica de ovinos infectados de Cajamarca, con una concentración proteica de 1 005 μg/μL, compuesta principalmente por proteνnas de peso molecular entre 1,2 y 170 KDa. Se detectaron bandas de 170; 150; 31; 24; 18-14 y 10 kDa. Con este antνgeno se desarrollσ una prueba de ELISA y se determinσ su punto de corte en 0,140. Se evaluσ 33 sueros de pacientes con fasciolosis confirmada por visualización de huevos en heces, 177 sueros de pacientes sin fasciolosis provenientes de áreas endémicas de Cajamarca y 88 sueros de pacientes con otras infecciones parasitarias y bacterianas. Se encontró una sensibilidad de 97,0%, especificidad de 96,6%, valor predictivo positivo de 78,1% y valor predictivo negativo de 99,6%. Se encontró reacción cruzada en 9/88 sueros evaluados. Se recomienda la implementación y uso de esta prueba para el diagnóstico de fasciolosis.Metabolic (excretion/secretion antigen was obtained from sheep infected with Fasciola hepatica, with a 1005 μg/μL of protein concentration, composed principally by proteins of molecular weight between 1.2 and 170 KDa. Bands of 170, 150, 31, 24, 18-14 and 10 kDa were detected. With this antigen an ELISA test was developed and the cut off was determined in 0.140. We evaluated 33 serums of patient with fascioliasis confirmed by visualization of eggs in feces, 177 serums of persons without fascioliasis from endemic rural areas of Cajamarca and 88 serums of patients with others parasitic and bacterial infections. We found a 97.0% of sensitivity, 96.6 specificity, 78.1% predictive positive value, 99.6 % predictive negative value. In 9/88 serums was found cross reactions. We recommended the implementation and use of this test for the fascioliasis diagnosis.

  15. Variation of snail's abundance in two water bodies harboring strains of Pseudosuccinea columella resistant and susceptible to Fasciola hepatica miracidial infection, in Pinar del Río Province, Cuba

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    Alfredo Gutiérrez

    2005-11-01

    Full Text Available The abundance of freshwater snails in two rural sites of Pinar del Río, Cuba, which harbor Pseudosuccinea columella susceptible and resistant to miracidia of Fasciola hepatica was followed for one year. Susceptible snails were found in the most anthropic site (IPA whereas the resistant population inhabited the most preserved one (El Azufre. Only two snail species coexisted with P. columella at IPA site (Physa cubensis and Tarebia granifera while five species were found at El Azufre, including an endemic from that province (Hemisinus cubanianus. Populations of both resistant and susceptible snails showed stable densities throughout the year, although the susceptible strain attained higher abundance. The highest densities were observed in April-May 2004 for the susceptible population whereas the resistant strain attained its highest abundance in January 2004. No record of Fossaria cubensis was made and the thiarid T. granifera occurred only at low densities. One of the sampled sites (IPA meets all the conditions for the first report of P. columella naturally infected with larvae of F. hepatica.

  16. Variation of snail's abundance in two water bodies harboring strains of Pseudosuccinea columella resistant and susceptible to Fasciola hepatica miracidial infection, in Pinar del Río Province, Cuba.

    Science.gov (United States)

    Gutiérrez, Alfredo; Hernandez, Dagmar F; Sánchez, Jorge

    2005-11-01

    The abundance of freshwater snails in two rural sites of Pinar del Río, Cuba, which harbor Pseudosuccinea columella susceptible and resistant to miracidia of Fasciola hepatica was followed for one year. Susceptible snails were found in the most anthropic site (IPA) whereas the resistant population inhabited the most preserved one (El Azufre). Only two snail species coexisted with P. columella at IPA site (Physa cubensis and Tarebia granifera) while five species were found at El Azufre, including an endemic from that province (Hemisinus cubanianus). Populations of both resistant and susceptible snails showed stable densities throughout the year, although the susceptible strain attained higher abundance. The highest densities were observed in April-May 2004 for the susceptible population whereas the resistant strain attained its highest abundance in January 2004. No record of Fossaria cubensis was made and the thiarid T. granifera occurred only at low densities. One of the sampled sites (IPA) meets all the conditions for the first report of P. columella naturally infected with larvae of F. hepatica.

  17. CE-Magnetic Resonance mammography for the evaluation of the contralateral breast in patients with diagnosed breast cancer; Ruolo della Risonanza Magnetica con Gd-BOPTA nella valutazione della mammella controlaterale in pazienti con tumore recentemente diagnosticato

    Energy Technology Data Exchange (ETDEWEB)

    Pediconi, Federica; Venditti, Fiammetta; Padula, Simona; Roselli, Antonella; Moriconi, Enrica; Catalano, Carlo; Passariello, Roberto [La Sapienza Univ., Roma (Italy). Dipartimento di scienze radiologiche; Giacomelli, Laura [La Sapienza Univ., Roma (Italy). Dipartimento di scienze chirurgiche

    2005-07-15

    Purpose. To evaluate the role of contrast-enhanced Magnetic Resonance Mammography (MRM) in the evaluation of the contralateral breast in patients with recently diagnosed breast cancer. Materials and methods. Fifty patients with proved unilateral breast cancer, with a negative contralateral breast at physical examination, ultrasound and mammography, were studied with a 1.5 T magnet (Siemens, Vision Plus, Germany). A bilateral breast surface coil was used. Dynamic 3D Flash T1-weighted sequences were acquired in the axial plane before and 0, 2, 4, 6 and 8 minutes after the administration of 0.1 mmol/kg of Gd-BOPTA at a flow rate of 2 ml/s followed by 10 ml of saline. The level of suspicion was reported on a scale from 0 to 5 following the BI-RADS classification, based on lesion morphology and kinetic features. The results were compared with the histological findings after biopsy or surgery. Results. Fourteen out of 50 patients (28%) had contralateral lesions identified on MRM. Biopsy was performed in four of them for suspicious lesions (BI-RADS 4) while 10 patients underwent surgery because of highly suggestive malignant lesions (BI-RADS 5). Histology diagnosed three fibroadenomas, 5 ductal carcinoma in situ, 2 lobular carcinomas in situ, 3 invasive ductal carcinomas and 1 invasive lobular carcinoma. Contrast enhanced MRM yielded no false negative and three false positives. Conclusions. Our results demonstrate a very good accuracy of Magnetic Resonance Mammography in the detection of synchronous contralateral cancer in patients with newly diagnosed breast cancer. Therefore, contrast-enhanced MRM could be introduced to screen patients with proven breast cancer before they under-go surgery. [Italian] Scopo. Definire il ruolo della Risonanza Magnetica nella valutazione della mammella contro laterale in pazienti con tumore della mammella recentemente diagnosticato. Materiale e metodi. Cinquanta pazienti con tumore monolaterale della mammella e mammella controlaterale

  18. Nuclear magnetic resonance in the evaluation of the disk luxation in the temporomandibular articulation conditioned for degenerative osseous changes; Resonancia magnetica nuclear en la valoracion de la luxacion discal en la articulacion temporomandibular condicionada por cambios degenerativos oseos

    Energy Technology Data Exchange (ETDEWEB)

    Marchegiani, Silvio; Guzman Urquhart, Romel; Marangoni, Alberto; Alvarez, Federico; Surur, Alberto [Sanatorio Allende, Cordoba (Argentina). Servicio de Diagnostico por Imagenes

    2006-07-01

    Purpose: The temporomandibular dysfunction is a common pathology. It appears with many symptoms as: joint pain, mechanic disturbance, and loss of mouth motion. The Magnetic Resonance Image is the chose method to study these alterations. The purposes of this paper are to demonstrate according to sex, the distribution of temporomandibular dysfunction and to determinant the relationships between the disc dislocation and the degenerative disease of the junction. Material and Method: 215 patients were evaluated between January 1999 and August 2004. An Elscint MRI 2 Tesla machine was used with Spin-echo (T1 and T2) Fat-sat sequences, in coronal, axial and sagittal planes. The follow alterations were evaluated: dislocation, degenerative disease of articular surface, osteochondritis, discal perforation, and retrodiscal lesions. Results: 175 of all showed pathologic alterations in IRM examination. The other 40 patients were excluded due to present a normal (MRI) study. The patients were 146 females and 29 men. The most common dislocation site was the anterior. Performance of the IRM was useful in all the cases to demonstrate degenerative changes in the temporomandibular joint (TMJ). And was a very precise relationship between the dislocation and degenerative disease of the joint. Conclusion: the female patient is the most frequently affected by temporomandibular joint pathology. The IRM is the selection method to study the TMJ. The degenerative changes are responsible for joint dislocation without reduction. (author) [Spanish] Proposito: Las disfunciones temporomandibulares son muy frecuentes. Los sintomas pueden ser: dolor a nivel de la articulacion, alteracion en la traslacion discocondilar (chasquido) y dificultad en la apertura bucal; dichos problemas conllevan a una consulta creciente de medicos clinicos como de odontologos. En la actualidad la Imagen por Resonancia Magnetica Nuclear (RM) es el estudio de eleccion. Los objetivos de este trabajo fueron demostrar la

  19. Purification and biochemical characterization of a 22-kDa stable cysteine- like protease from the excretory-secretory product of the liver fluke Fasciola hepatica by using conventional techniques.

    Science.gov (United States)

    Hemici, Ahmed; Benerbaiha, Roumaila Sabrina; Bendjeddou, Dalila

    2017-11-15

    This study describes the purification and characterization of a stable protease activity isolated from Fasciola hepatica adult worms maintained in vitro by employing acetone precipitation (40-60%) followed by a gel filtration through Sephadex G-100 and DEAE- cellulose ion exchange column. Through this three-step purification, the enzyme was purified 11-fold with a specific activity of 1893.9U/mg and 31.5% recovery. After the final ultrafiltration step, the purification fold was increased up to 13.1 and the overall activity yield reached a rate of 18.8%. The MW of the purified protease was estimated by reducing SDS-PAGE to be 22kDa while the proteolytic activity detection was carried out by zymography on non-denaturing SDS-PAGE containing the casein as substrate. Using this substrate, the protease showed extreme proteolytic activity at pH 5.5 and temperature 35-40°C and was highly stable over a wide range of pH, from 5.0 to 10.0. In addition to its preference for the Z-Phe-Arg-AMC fluorogenic substrate resulting in maximum proteolytic activity (99.7%) at pH 7.0, the pure protease exhibited highest cleavage activity against hemoglobin and casein substrates at pH 5.5 (85.6% and 82.8%, respectively). The K m values obtained for this protease were 5.4, 13, 160 and approximately 1000μM using respectively the fluorogenic substrate Z-Phe-Arg-AMC, hemoglobin, casein and albumin. The protease activity was completely inhibited either by E-64 inhibitor (5mM) or iodoacetamide (10mM), indicating its cysteine nature. The usefulness of the purified protease as an antigen was studied by immunoblotting. Thus, sera from sheep experimentally infected with F. hepatica recognized the protease band at 2 weeks post-infection (WPI) and strongly at 7 WPI. The early detection of antibodies anti- F. hepatica suggests the application of this molecule as a specific epitope for the serodiagnosis of fascioliasis disease. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Comparação de kits ELISA® comerciais para anticorpos no soro e leite com um teste coproparasitológico em bovinos naturalmente infectados por Fasciola hepatica

    Directory of Open Access Journals (Sweden)

    Cíntia das C. Bernardo

    2013-01-01

    Full Text Available A fasciolose é uma enfermidade causada por um trematoda que acomete o fígado principalmente de ruminantes domésticos, podendo parasitar o homem e seu diagnóstico é realizado rotineiramente por exames coproparasitológicos. O objetivo do presente estudo foi comparar kits comerciais de ELISA para anticorpos no soro e leite com um teste coproprarasitológico em bovinos naturalmente infectados por Fasciola hepatica. Foram coletadas amostras de fezes (92 sangue (92 e leite (43 de bovinos provenientes de propriedades de gado leiteiro do município de Jerônimo Monteiro, sul do Estado do Espírito Santo. As amostras de fezes coletadas foram processadas pela técnica de sedimentação fecal para ovos de F. hepatica, utilizada como padrão ouro para as análises. Amostras de sangue e de leite foram processadas segundo a orientação do fabricante dos respectivos Kits ELISA comerciais testados. Utilizou-se o c² de McNemar para comparação estatística e calcularam-se a sensibilidade e especificidade, valores preditivos e kappa. Os resultados obtidos mostraram que as frequências de positividade pelo uso dos kits ELISA comerciais de soro e de leite diferiram significativamente (p<0,0001 em relação ao exame coproparasitológico. A sensibilidade dos Kits foi de 100%, porém possuíram baixa especificidade, 42,85 e 30% para o soro e leite respectivamente. O coeficiente de kappa mostrou concordância sofrível para os testes de soro (0,33 e de leite (0,21. Os valores preditivos positivos dos kits para soro e leite foram, respectivamente, 44,61 e 38,23% e, os valores preditivos negativos de 100% para ambos os testes. Apesar da maior sensibilidade dos kits ELISA comerciais e, destes apresentarem diferença em relação ao exame coproparasitológico na detecção dos animais positivos para F. hepatica, a escolha de um teste diagnóstico deve considerar o custo benefício. Quando se trata da presença de parasitismo em rebanhos, o tratamento é aplicado

  1. Comparación de las técnicas Kato-Katz, TSET y TSR en el diagnóstico de infección por Fasciola hepatica en humanos

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    Nelson Uribe Delgado

    2012-12-01

    Full Text Available Introducción: La Fasciolosis es una enfermedad causada por el parásito Fasciola hepatica, que afecta primordialmente a bovinos, ovinos, caprinos y en algunas zonas de forma endémica a los humanos. En la investigación clínica y epidemiológica de fasciolosis humana es importante contar con técnicas coprológicas de diagnóstico de alta sensibilidad y especificidad. Objetivo: realizar la comparación de tres técnicas coprológicas para el diagnóstico de infección por Fasciola hepatica en humanos. Metodología: se procesaron 200 gramos de materia fecal de humanos contaminada experimentalmente con huevos de F. hepatica que se obtuvieron de análisis anteriores con muestras de ganado bovino y ovino. La muestra se dividió en 10 partes iguales; a cada una de estas muestras se les realizó la técnicas de Kato-Katz, técnica de sedimentación espontánea en tubo (TSET y técnica de sedimentación rápida (TSR. Finalmente, la lectura de las muestras se basó en el diseño metodológico del doble ciego. Resultados: se encontró que la técnica de sedimentación rápida fue la más sensible de las tres evaluadas, detectándose positividad en 7,5 muestras de 10 analizadas; seguida por la técnica de Kato-Katz con 4,5 muestras y en último lugar la técnica de sedimentación espontánea en Tubo que mostró solo un resultado positivo. Conclusión: en este trabajo se encontró que la técnica de sedimentación rápida constituye la herramienta de elección para el profesional porque es la más sensible de las tres; además de ser sencilla, económica y de fácil aplicación en laboratorios de niveles uno y dos de complejidad para realizar un diagnóstico satisfactorio de fasciolosis humana. Salud UIS 2012; 44 (3: 7-12

  2. Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados

    Directory of Open Access Journals (Sweden)

    María Alejandra De Almeida

    2007-02-01

    Full Text Available Fascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE and the adult worm vomit (FhAWV in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F, other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis.Fasciolíase é uma doença emergente/re-emergente transmitida por vetores com a distribuição sabidamente mais ampla. Existem aproximadamente 17 milhões de pessoas infectadas em todo mundo, sendo a região andina a área mais afetada. Há uma necessidade importante para desenvolver ferramentas diagnósticas sensíveis e específicas para tratar cedo os pacientes e para evitar complicações. Neste trabalho avaliamos a resposta imune de seres humanos infectados comparando a duas preparações antigênicas: o extrato solúvel total (FhTSE e o vômito (FhAWV do verme adulto a fim de identificar as frações antigênicas específicas para

  3. Análise do transcriptoma do estágio invasivo de Fasciola hepatica e sua contribuição na compreensão dos mecanismos moleculares envolvidos no processo de infecção

    OpenAIRE

    Martín Pablo Cancela Sehabiague

    2010-01-01

    Fasciola hepatica é um trematódeo parasita e o agente causador da fasciolose. Esta zoonose causa perdas importantes na produção agropecuária e tem uma crescente incidência na saúde dos seres humanos, principalmente em países em desenvolvimento. Mesmo que existem drogas fasciolicidas, estas não evitam a reinfecção e o surgimento de resistência e, portanto são necessárias novas estratégias de controle. A compreensão dos mecanismos moleculares que envolvem a relação parasito-hospedeiro e os proc...

  4. Nuclear magnetic resonance spectroscopy in biomedicine. Course organized by the Istituto Superiore di Sanita`. Marciana Marina (Isola d`Elba), September 18-23, 1995; Spettroscopia di risonanza magnetica nucleare in biomedicina. Corso organizzato dall`Istituto Superiore di Sanita`. Marciana Marina (Isola d`Elba), 18-23 settembre 1995

    Energy Technology Data Exchange (ETDEWEB)

    Luciani, A M; Rosi, A [ed.; Istituto Superiore di Sanita` , Rome (Italy). Lab. di Fisica

    1997-12-01

    Nuclear magnetic resonance is a powerful technique that can be used in a wide range of applications, such as the structural characterization of high molecular weight molecules, conformational studies on enzymes in solution, enzyme-substrate or DNA-protein interactions, monitoring of cell metabolism in vivo, and for diagnostic purposes, employing spectroscopic and imaging techniques. This course was organized in order to introduce the participants to the fundamentals of NMR spectroscopy, and offer practical advice on performing NMR experiments on cell systems, cell and tissue extracts and animal models. The main implications regarding human experiments were also discussed. Finally the quantification of information and the interpretation of data were considered with regard to the main nuclei observed. [Italiano] La risonanza magnetica nucleare e` una delle tecniche spettroscopiche che meglio risponde all`ampio spettro di condizioni imposto dalla ricerca in biofisica e biomedicina. Il campo applicativo di questa tecnica copre un intervallo molto ampio che va dalla caratterizzazione di strutture di macromolecole con elevato peso moleculare, a studi di tipo conformazionale su enzimi in soluzione e sull`interazione enzima-substrato o DNA-proteine, fino al monitoraggio in vivo del metabolosmi cellulare e alla diagnostica medica con spettroscopia ed imaging. Il corso e` stato articolato in modo da presentare ai partecipanti nozioni di base della spettroscopia RMN, aspetti pratici della realizzazione di esperimenti di spettroscopia in sistemi cellulari, in estratti cellulari e tissutali e in animali da esperimento, nonche` le principali considerazioni relative agli esperimenti nell`uomo. Sono state affrontate tematiche relative alla quantificazione dell`informazione e all`interpretazione dei dati in relazione ai principali nuclei osservati.

  5. Lanthanide oxide and phosphate nanoparticles for thermometry and bimodal imaging =

    Science.gov (United States)

    Debasu, Mengistie Leweyehu

    . Finalmente, estudam-se as propriedades de fotoluminescencia correspondentes as conversoes ascendente e descendente de energia em nanocristais de (Gd,Yb,Tb)PO4 sintetizados por via hidrotermica. A relaxividade (ressonancia magnetica) do 1H destes materiais sao investigadas, tendo em vista possiveis aplicacoes em imagem bimodal (luminescencia e ressonancia magnetica nuclear).

  6. Purificación de la fracción antigénica 27-28 KDa a partir del antígeno metabólico secretado-excretado de Fasciola hepatica

    Directory of Open Access Journals (Sweden)

    Isidro Antitupa

    Full Text Available En el presente estudio, las fracciones antigénicas de 27-28 KDa de Fasciola hepatica fueron purificadas por cromatografía de exclusión molecular para su aplicación en el diagnóstico de la fascioliasis humana. Se obtuvieron antígenos de excreción y secreción a partir de fasciolas adultas vivas obtenida de hígado de ovino y bovino, y cultivados en medio mínimo esencial. La reactividad y eficacia del antígeno purificado fueron evaluadas por la prueba de inmunoblot empleando cuatro sueros con fascioliasis humana; cuatro sueros con otras parasitosis, y dos sueros negativos. Se concluye que las fracciones antigénicas purificadas no presentan reacción cruzada con otras parasitosis, por inmunoblot, por lo que se considera a las proteínas purificadas como potenciales candidatas a ser utilizadas para el diagnóstico de fascioliasis humana

  7. Scanning Study of 700 Livers Evaluation of Existing Diagnostic Procedures; Etude Scintigraphique de 700 Foies (Evaluation des Procedures Actuelles de Diagnostic); Issledovanie pecheni 700 bol'nykh s pomoshch'yu skennirovaniya; Exploracion Hepatica de 700 Pacientes (Evaluacion de los Metodos Actuales De Diagnostico); Exploracion Hepatica de 700 Pacientes (Evaluacion de los Metodos Actuales de Diagnostico)

    Energy Technology Data Exchange (ETDEWEB)

    Czerniak, P. [Radium and Isotope Institute, Government Hospital, Tel-Hashomer (Israel)

    1964-10-15

    objetiva del diagnostico. Esto ha permitido evaluar los ensayos realizados. Para la exploracion del higado se utilizo 198Au, Rosa Bengala marcado con {sup 131}I y polivinilpirrolidona marcada tambien con {sup 131}I. Ademas de la exploracion anteroposterior, se recurrio a tecnicas especiales como la exploracion estereoscopica en dos o tres planos y al empleo combinado del {sup 198}Au y el Rosa Bengala marcado con {sup 131}I para determinar los coeficientes relativos de fijacion de estos radioisotopos en el hfgado. Los pacientes habfan sido distribuidos en ocho grupos clinicos con miras a la compilacion y evaluacion de los resultados del diagnostico. En la memoria se presenta un resumen de estos resultados. Cada grupo clfnico se analizo por separado. Por ejemplo, se detecto una lesion macroscopica en el 95% de los 65 casos de equinococosis. La radiografia solo permitio descubrir calcificaciones en el 35% de estos casos, pero tambien en el 14% de los otros casos de afecciones hepaticas. Las reacciones de Weinberg y Casoni fueron positivas en el 64% de los casos de equinococosis, pero tambien en el 22 por ciento de las otras enfermedades. En loque respecta al cancer del hfgado, se detecto una lesion macroscopica en el 44% de los casos, se obtuvo un centelleograma velado en el 30% y se observaron modificaciones morfologicas en el 29%. En dos de los 158 casos de tumores malignos, el centelleograma parecia falsamente normal, pero en el 40% de estos mismos casos los resultados obtenidos en el laboratorio fueron negativos. Con la tecnica de exploracion estereoscopica en dos o tres planos se obtuvieron datos nuevos o complementarios sobre el estado del higado en el 45% de los casos. La centelleografia se utilizo tambien para el control ulterior de los resultados. Se han hecho las siguientes observaciones: 1. Despues de una equinococotomia persiste una cicatriz caracteristica en forma de lesion macroscopica. 2. Tres meses despues del tratamiento de un absceso amebico, se produce una

  8. The influence of hypothyroidism on liver regeneration: an experimental study in rats A influência do hipotireoidismo na regeneração hepatica: estudo experimental em ratos

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Pessole Biondo-Simões

    2007-01-01

    Full Text Available BACKGROUND: The influence of hypothyroidism in liver regeneration has been a controversial opinions. PURPOSE: The aim of this study is to identify the relationship between hypothyroidism and liver regeneration in rats. METHODS: Forty male Wistar rats divided into two groups of 20 specimens each. One group (C consisted of euthyroid rats, and the other (H of hypothyroid rats. All the animals were anesthetized with xylazine and ketamine and subjected to a longitudinal incision in the anterior cervical region. The thyroid was completely resected in group H and left intact in group C. Ten days after the first surgery, both groups of rats were weighed and submitted to partial hepatectomy, in which the left lateral and median lobes were resected and weighed. Examinations were carried out after 24 hours and, on day 7, using 3 methods: KWON et al.'s formula to identify increase in volume; mitotic figure count in five fields; and the percentage of PCNA-positive nuclei in five fields. RESULTS: Using KWON's formula, the regeneration rate for Group C after 24 hours was 58.49% whereas that for Group H was 50.42% (p=0.0165. After 7 days, the regeneration rate for Group C was 93.04% and Group H 93.74% (p=0.2165. The average number of mitotic figures after 24 hours was 14 ± 1.5 for Group C and 9.8 ± 2.2 for Group H (p=0,00016. After 7 days the corresponding figures were 5.4 ± 1.1 and 5.1 ± 1.2 (p=0,6343. The average number of PCNA-positive nuclei after 24 hours was 13.55 ± 3.84 in Group C and 7.7 ± 2.11 in Group H (p =0,0006. The corresponding figures after 7 days were 3.5 ± 2.39 for Group C and 4.11 ± 1.90 for Group H (p>0.05. CONCLUSION: We conclude that hypothyroidism in rats causes a delay in hepatic regeneration in the first 24 hours, but that after seven days the rate of regeneration is equal to that in euthyroid rats.BACKGROUND: A influência do hipotireoidismo na regeneração hepatica tem opiniões controvérsas. OBJETIVO: Identificar a rela

  9. Формирование овогоний трематоды Fasciola hepatica Linneus, 1758

    OpenAIRE

    Соколина Ф.М.; Рахимов И.И.; Игнатьев Г.

    2012-01-01

    С помощью растровой электронной микроско-пии исследовали покров тела мариты Fasciola hepati-ca Linneus, 1758. Проведен его ренгеноспектральный (электронно-зондовый) микроанализ и сравнитель-ный анализ топографии щетинок половозрелых осо-бей и марит, только что покинувших капсулы адоле-скарий. Установлен элементный состав покровов те-ла фасциолы, щетинок и полипов на ее поверхности...

  10. Properties of self-assembled diluted magnetic semiconductor nanostructures =

    Science.gov (United States)

    Ankiewicz, Amelia Olga Goncalves

    Este trabalho centra-se na investigacao da possibilidade de se conseguir um semicondutor magnetico diluido (SMD) baseado em ZnO. Foi levado a cabo um estudo detalhado das propriedades magneticas e estruturais de estruturas de ZnO, nomeadamente nanofios (NFs), nanocristais (NCs) e filmes finos, dopadas com metais de transicao (MTs). Foram usadas varias tecnicas experimentais para caracterizar estas estruturas, designadamente difraccao de raios-X, microscopia electronica de varrimento, ressonancia magnetica, SQUID, e medidas de transporte. Foram incorporados substitucionalmente nos sitios do Zn ioes de Mn2+ e Co2+ em ambos os NFs e NCs de ZnO. Revelou-se para ambos os ioes dopantes, que a incorporacao e heterogenea, uma vez que parte do sinal de ressonancia paramagnetica electronica (RPE) vem de ioes de MTs em ambientes distorcidos ou enriquecidos com MTs. A partir das intensidades relativas dos espectros de RPE e de modificacoes da superficie, demonstra-se ainda que os NCs exibem uma estrutura core-shell. Os resultados, evidenciam que, com o aumento da concentracao de MTs, a dimensao dos NCs diminui e aumentam as distorcoes da rede. Finalmente, no caso dos NCs dopados com Mn, obteve-se o resultado singular de que a espessura da shell e da ordem de 0.3 nm e de que existe uma acumulacao de Mn na mesma. Com o objectivo de esclarecer o papel dos portadores de carga na medicao das interaccoes ferromagneticas, foram co-dopados filmes de ZnO com Mn e Al ou com Co e Al. Os filmes dopados com Mn, revelaram-se simplesmente paramagneticos, com os ioes de Mn substitucionais nos sitios do Zn. Por outro lado, os filmes dopados com Co exibem ferromagnetismo fraco nao intrinseco, provavelmente devido a decomposicao spinodal. Foram ainda efectuados estudos comparativos com filmes de ligas de Zn1-xFexO. Como era de esperar, detectaram-se segundas fases de espinela e de oxido de ferro nestas ligas; todas as amostras exibiam curvas de histerese a 300 K. Estes resultados suportam a

  11. Immunity to Fasciola hepatica in the rat

    International Nuclear Information System (INIS)

    Armour, J.; Dargie, J.D.

    1974-01-01

    Experiments were carried out which demonstrated an acquired immunity to Fasciola hapatica in the rat. It was shown that this immunity could be transferred to recipients using either lymphoid cells or serum from infected donor rats. The extent of the protection obtained by cells appeared to be related to the quantity and persistence of the antigenic stimulus in the donor. Likewise, the degree of immunity conferred by immune serum was dependent upon the volume transferred. The significance of these results in relation to the mechanism of immunity to fascioliasis is discussed

  12. Estandarización de la técnica de Western blot para el diagnóstico de la fasciolosis humana utilizando antígenos de excreción-secreción de Fasciola hepática Western blot technique standardization of the diagnosis of human fasciolosis using Fasciola hepatica excreted-secreted antigens

    Directory of Open Access Journals (Sweden)

    Hermes Escalante

    2011-09-01

    Full Text Available Objetivos. Evaluar la eficacia de la técnica de electroinmunotransferencia (EITB o Western blot utilizando antígenos de excreción-secreción de las formas adultas de Fasciola hepatica (Fh E/S Ag para el diagnóstico de la fasciolosis humana. Materiales y métodos. Los antígenos fueron obtenidos a las 18 horas de incubación en medio Minimum Essential Eagle y preparados a la concentración proteica de 0,15 ug/uL; los cuales, al ser enfrentados con un pool de sueros de pacientes con fasciolosis confirmada por el hallazgo de huevos del parásito en las heces, se detectaron los antígenos de 10, 12, 17, 23, 27, 30, 36, 43, 66 y 136 KDa, con los cuales se desarrolló la técnica de Western blot. La sensibilidad se evaluó empleando sueros de 67 pacientes con fasciolosis, y la especificidad con sueros de 57 pacientes con otras parasitosis y diez sueros de personas no parasitadas. Resultados. De los 67 sueros, 64 reaccionaron con la banda de 23 KDa y 61 con la banda de 17KDa. Estas dos bandas no fueron detectadas por ninguno de los sueros de pacientes con otras parasitosis, ni de personas no parasitadas, siendo por ello consideradas como específicas y diagnósticas. Conclusiones. La sensibilidad de la prueba, utilizando las bandas de 17 y 23 KDa, fue de 95,5 % cuando se presenta reacción positiva en una o en las dos bandas, siendo la especificidad para estos dos antígenos de 100 % con un valor predictivo positivo de 100 % y un valor predictivo negativo de 95,71 %.Objectives. To evaluate the performance of the enzyme-linked immunoelectrotransfer blot assay (EITB, Western blot using excretory/secretory antigens from adult forms of Fasciola hepatica (Fh E/S Ag for the diagnosis of human fasciolosis. Materials and methods. Antigens were obtained after 18 hours of incubation in culture medium Minimum Essential Eagle, prepared at a protein concentration of 0.15 ug/uL and run against a pool of sera of patients with proven fasciolosis (confirmed by the

  13. Retrospective dosimetry of nail by Electron Paramagnetic Resonance; Dosimetria retrospectiva de unha por Ressonancia Paramagnetica Eletronica

    Energy Technology Data Exchange (ETDEWEB)

    Giannoni, Ricardo A., E-mail: giannoni@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Rodrigues Junior, Orlando, E-mail: rodrijr@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2015-07-01

    The purpose of this study is to characterize samples of human nails, subjected to irradiation of high doses through Technical Electron Paramagnetic Resonance (EPR). The goal is to establish a dose/response relationship in order to assess dose levels absorbed by individuals exposed in radiation accidents situations, retrospectively. Samples of human nails were irradiated with gamma radiation, and received a dose of 20 Gy. EPR measurements performed on samples before irradiation identified EPR signals associated with defects caused by the mechanical action of the sample collection. After irradiation other species of free radicals, associated with the action of gamma radiation, have been identified.

  14. Technical competence in cardiovascular magnetic resonance and computed tomography; Competencia tecnica em ressonancia e tomografia cardiovascular

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, Juliano Lara; Shiozaki, Afonso Akio; Azevedo Filho, Clerio Francisco de; Rochitte, Carlos Eduardo; Pinto, Ibraim Marciarelli Francisco; Lopes, Marly Maria Uellendahl; Schvartzman, Paulo Roberto, E-mail: jlaraf@fcm.unicamp.br [Universidade de Campinas (UNICAMP/GERT) SP (Brazil). Grupo de Estudo em Ressonancia e Tomografia Cardiovascular

    2009-10-15

    Cardiovascular magnetic resonance and computed tomography have evolved as very practical and useful techniques applied in clinical cardiology. Due to their rapid acceptance in the cardiology community and widespread use, training of both cardiologists and radiologists on this subspecialty has not been homogeneous so far. This in part explains significant differences observed in the diverse background found in today’s practicing physicians who execute these exams. In order to guide training facilities as well as both payers, contractors and general cardiologists ordering the exam, this document provides a minimum standard that should be accomplished by all physicians who pursue education in the field and for those who already practice in it. The clinical competences listed in this statement are by no means thorough but should be required by all those involved in cardiovascular magnetic resonance and computed tomography as the customary requirements for current and future practitioners. (author)

  15. Evaluation of oxidative status in patients with Fasciola hepatica ...

    African Journals Online (AJOL)

    Their Total antioxidant capacity status (TAC), total oxidant status (TOS) and catalase were measured in them and oxidative stress index (OSI) was calculated. These measurements were also taken for the control group and the values were compared. Results: Plasma levels of total TOS and OSI were significantly increased ...

  16. Rhodoquinone is synthesized de novo by Fasciola hepatica

    NARCIS (Netherlands)

    Hellemond, van J.J.; Luijten, M.; Flesch, F.M.; Gaasenbeek, C.P.H.; Tielens, A.G.M.

    1996-01-01

    Most adult parasitic helminths have an anaerobic energy metabolism in which fumarate is reduced to succinate by fumarate reductase. Rhodoquinone (RQ) is an essential component of the electron transport associated with this fumarate reduction, whereas ubiquinone (UQ) is used in the aerobic energy

  17. Apparent Triclabendazole-Resistant Human Fasciola hepatica Infection, the Netherlands

    NARCIS (Netherlands)

    Winkelhagen, Annemarie J. S.; Mank, Theo; de Vries, Peter J.; Soetekouw, Robin

    2012-01-01

    TO THE EDITOR: In December 2007, a 71-year-old sheep farmer sought care with a 4-month history of intermittent right upper quadrant pain, night sweats, anorexia, and a 5-kg weight loss. His medical history was unremarkable, and he had not traveled outside the Netherlands for ≈30 years. Physical

  18. Studies on Colombian Cryptogams. VIII. The genus Jensenia Lindb. (Hepaticae)

    NARCIS (Netherlands)

    Gronde, van der Keympe

    1980-01-01

    A study of recent material of the dendroid thallose liverwort genus Jensenia (Metzgeriales) from the Colombian Andes revealed the existence of three taxa in the area: J. erythropus (Gott.) Grolle var. erythropus, J. erythropus var. nobandae van der Gronde var. nov. and J. florschuetzii van der

  19. Two New Epiphyllous Leptolejeunea (Hepaticae: Lejeuneaceae from Eastern Himalaya, India

    Directory of Open Access Journals (Sweden)

    Monalisa Dey

    2010-11-01

    Full Text Available Two new epiphyllous species of the genus Leptolejeunea (Spruce Schiffn., viz. L. mirikana M. Dey et D. K. Singh sp. nov. and L. udarii M. Dey et D. K. Singh sp. nov., are described from Darjeeling district of West Bengal and East district of Sikkim respectively in Eastern Himalaya, India. While, the former is characterized by obovate leaf lobes with acute – subacute leaf apices and entire margin; rectangular – trapezoid underleaves with rectangular – lanceolate lobes which are 3-4 cells long, 2-3 cells wide, or rarely 1 cell wide at apex, and crenulate – denticulate margin; presence of ocelli in female bracts and bracteoles and obconical perianth with truncate apices and 5 obliquely – horizontally spreading horn-like keels extending from apex to 1/2-3/5 of perianth length, the latter is distinct in having small length-breadth ratio of the leaves ranging between 1.2:1-1.3:1; obovate leaf lobes with subacute apices; terminal as well as intercalary androecia with male bracteoles present throughout; numerous ocelli scattered on female bracts, bracteoles and perianth and obconical – campanulate perianth with 5 smooth, erect – obliquely spreading horn-like keels. A key to the Indian species of the genus has been provided.

  20. Treatment of effluent containing uranium with magnetic zeolite; Tratamento de efluente contendo uranio com zeolita magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Craesmeyer, Gabriel Ramos

    2013-07-01

    Within this work, a magnetic-zeolite composite was successfully synthesized using ferrous sulfate as raw material for the magnetic part of the composite, magnetite, and coal fly ash as raw material for the zeolitic phase. The synthesis of the zeolitic phase was made by alkali hydrothermal treatment and the magnetite nanoparticles were obtained through Fe{sup 2+} precipitation on alkali medium. The synthetic process was repeated many times and showed good reproducibility comparing the zeolitic nanocomposite from different batches. The final product was characterized using infrared spectroscopy, powder X-ray diffraction, X-ray fluorescence, scanning electron microscopy with coupled EDS. Specific mass, specific surface area and other physicochemical proprieties. The main crystalline phases found in the final product were magnetite, zeolites types NaP1 and hydroxysodalite, quartz and mullite, those last two remaining from the raw materials. Uranium removal capacity of the magnetic zeolite composite was tested using batch techniques. The effects of contact time and initial concentration of the adsorbate over the adsorption process were evaluated. Equilibrium time was resolved and the following kinetics and diffusion models were evaluated: pseudo-first order kinetic model, pseudo-second order kinetic model and interparticle diffusion model. A contact time of 120 min turned out to be enough to reach equilibrium of the adsorption process. The rate of adsorption followed the pseudo-second order model and the intra particle diffusion did not turn out to be a speed determinant step. Two adsorption isotherms models, the Langmuir model and the Freundlich model, were also evaluated. The Langmuir model was the best fit for the obtained experimental data. Using the best fitted adsorption isotherm and kinetic model, the theoretical maximum adsorption capacity of uranium over the composite was determined for both models. The maximum removal capacity calculated was 20.7 mg.g{sup -1} for the Langmuir isotherm and 23.4 mg.g{sup -1} for the pseudo-second order model. The experimental value attained was 23,3 mg.g{sup -1}. (author)

  1. Le leucodistrofie: aspetti clinici e quadri con Tomografia Computerizzata e con Risonanza Magnetica

    International Nuclear Information System (INIS)

    Magnaldi, S.

    1991-01-01

    Leukodystrophies are inherited white matter diseases due to abnormalities occurring in myelin synthesis and/or maintenance. The most common types of these rare childhood conditions are represented by adrenoleukodystrophy, metachromatic leukodystrophy, Canavan's, Alexander's, Krabbe's, and Pelizaeus-Merzbacher's diseases. Most of them are lethal during childhood, with the exception of the adrenoleukodystrophy-adrenomyeloneuropathy complex, which sometimes, during its early phases, may be cured with a dietary therapy. The aims of this paper are: 1) the description of inheritance factors, pathogenesis, pathological and clinical findings of each of the most frequent childhood leukodystrophies; 2) the description of the most common patterns of these conditions on CT and MR imaging; 3) the evaluation of the diagnostic capabilities of these two imaging techniques and the comparison of their results. Finally, some of the therapies suggested for the mild forms of these conditions are discussed. The evaluation of leukodystrophic patients with CT and MR imaging shows both imaging modalities to have high sensitivity, thanks to the detection of abnormally myelinated areas, which appear hypodense on CT and Hypertense on T2-weighted MR images. Frequently, both imaging modalities exhibit high specificity as well: they allow a differential diagnosis between the different types through the demonstration of their location in the early stages and of their mode of spread. The most typical example is represented by adrenoleukodystrophy, which is the most common type of leukodystrophy: the frequent occipito-parietal onset and the anterior and caudal progression allow a correct diagnosis to be made on CT and MR images in most cases. The comparison between CT and MR findings demonstrates a slight superiority of the latter: multiplanarity and high contrast resolution make MR imaging more sensitive than CT in the detection of the both caudal spread and involvement of optic and acoustic nervous pathways. Furthermore, MR imaging allows a safe follow-up in children with leukodystrophy

  2. Magnetic resonance imaging of the larynx; Resonancia magnetica de la laringe

    Energy Technology Data Exchange (ETDEWEB)

    Oleaga, L. [Hospital de Basurto. Bilbao (Spain)

    2001-07-01

    Larynx and hypopharynx represent difficult anatomical structures to evaluate by imaging. Pathological processes inflammatory conditions and neoplasms are frequently found specially neoplasms. The most common tumor originating in this region is squamous cell carcinoma that represents 90%, of malignancies, magnetic Resonance Imaging (MRI) is used for staging of those tumors. In our study we evaluate the technique used for MRI studies as well as the anatomy of the larynx, hypopharynx and nodes and the different classification system used for neck nodes. We also analyze the different types of neoplasm that can be found being and malignant and the use of MRI for staging neoplasms. Clinical and laryngoscope staging of laryngeal tumors is not accurate if fails to demonstrate deep submucosal and laryngeal spaces invasion (pre-epi glottic. para laryngeal, para glottic, anterior commissures posterior commissure and sub glottis) as well as cartilage infiltration and extra laryngeal extension. We need accurate imaging diagnostic methods like MRI to evaluate the extension of tumors. (Author) 38 refs.

  3. Stating of cervical carcinoma using magnetic resonance imaging; Estadificacion del carcinoma de cervix por resonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Oleaga, L.; Vela, M. C.; Grande, J.; Cura del, J. L.; Grande, D. [Hospital de Basurto. Bilbao (Spain)

    1999-07-01

    The infiltration of the parametrium represents one of the most important factors that determine the prediction and treatment of cervical carcinoma. Our objetive is to evaluate the utility of magnetic resonance imaging (MRI) in the staging of cervical carcinomas, to establish the reliability of this technique and to carry out a comparative study of the sequences used to demonstrate the parametrial invasion. We have carried out a retrospective study on 44 patients diagnosed with cervix neoplasia, using clinical exploration and performing a biopsy. the MRI studies have been carried out using a 1 Tesla magnet and the sequences used have been SE T1, Se proton density (PD) and T2 and dynamic GRE after administering gadolinium intravenously in the axial and sagital projections. The stages determined by MRI have been compared to the anatomopathological stages of the surgical specimens in cases where surgery was carried out and with the clinical stage in cases where no radical surgery was carried out. A diagnosis value of MRI has been determined to demonstrate the parametrial invasion, comparing the SE T2 sequence with the dynamic GE sequence with gadolinium. We calculate the volume of the tumour in the MRI studies to evaluate the difference of the volume between patients with tumoral stages that are clinically surgical and not surgical. MRI determines the invasion of the parametrium with a sensitivity of 88.8%, a specificity of 80% a positive value of 76.1%, a negative predictive value of 90.9% and a reliability of 83.7%. For the SE T2 sequences the sensitivity was 86.6%, the specifity 80%, the posistive predictive value 81.25%, the negative predictive value 85.7% and the reliability 83.3%. For the dynamic GE sequence with intravenous gadolinium the sensitivity was 86.6%, the specifity 86.6%, the posistive predictive value 86.6%, the negative predictive value 86.6% and the reliability 86.6%. The use of the dynamic GE sequence after the intravenous administration of gadolinium in patients with negative SE T2 sequence for the infiltration of the parametrium increased the negative predictive value of the combination of both series to 95.7%. The volume average of the tumor in patients with clinically surgical stages was 14.21 cm''3 and the average in patients with non surgical stages was 69.79 cm''3, the difference was statistically significant (p<0.0001). In the diagnosis of parametrial invasion, in the cervix carcinomas the SE T2 sequences have shown a high reliability. the use of gadolinium intravenously can improve the negative predictive value of the test, allowing to decide which patients need surgery with greather reliability. (Author) 18 refs.

  4. Functional magnetic resonance maps obtained by personal computer; Mapas de resonancia magnetica funcional obtenidos con PC

    Energy Technology Data Exchange (ETDEWEB)

    Gomez, F. j.; Manjon, J. V.; Robles, M. [Universidad Politecnica de Valencia (Spain); Marti-Bonmati, L.; Dosda, R. [Cinica Quiron. Valencia (Spain); Molla, E. [Universidad de Valencia (Spain)

    2001-07-01

    Functional magnetic resonance (fMR) is of special relevance in the analysis of certain types of brain activation. The present report describes the development of a simple software program for use with personal computers (PCs) that analyzes these images and provides functional activation maps. Activation maps are based on the temporal differences in oxyhemoglobin in tomographic images. To detect these differences, intensities registered repeatedly during brain control and activation are compared. The experiments were performed with a 1.5-Tesla MR unit. To verify the reliability of the program fMR studies were carried out in 4 healthy individuals (12 contiguous slices, 80 images per slice every 3.1 seconds for a total of 960 images). All the images were transferred to a PC and were processed pixel by pixel within each sequence to obtain an intensity/time curve. The statistical study of the results (Student's test and cross correlation analysis) made it possible to establish the activation of each pixel. The images were prepared using spatial filtering, temporal filtering, baseline correction, normalization and segmentation of the parenchyma. The postprocessing of the results involved the elimination of single pixels, superposition of an anatomical image of greater spatial resolution and anti-aliasing. The application (Xfun 1.0, Valencia, Spain) was developed in Microsoft Visual C++5.0 Developer Studio for Windows NT Workstation. As a representative example, the program took 8.2 seconds to calculate and present the results of the entire study (12 functional maps). In the motor and visual activation experiments, the activation corresponding to regions proximal to the central sulcus of the hemisphere contralateral to the hand that moved and in the occipital cortex were observed. While programs that calculate activation maps are available, the development of software for PCs running Microsoft Windows ensures several key features for its use on a daily basis: it is easy to employ and has powerful computing capabilities. The program described here is capable of differentiating between the regions of the brain that are activated in response to a stimulus and those that remain unaltered. (Author) 19 refs.

  5. Magnetic resonance in the dilation of terminal ventricle; Resonancia magnetica en la dilatacion del ventriculo terminal

    Energy Technology Data Exchange (ETDEWEB)

    Eulatem d, R. G.; Martinez, M. E.; Oleaga, L.; Grande, D.

    2001-07-01

    Describe de Magnetic Resonance (MR) findings in four cases of terminal ventricle dilation. We have studied four patients, all of them women, whose mean age range was 39 years. The studies were performed with a 1 Tesla magnet and the sequences used were sagittal and axial T1 and T2 weighted and sagittal and axial T1 weighted after the administration of gadolinium. the follow-up was clinical and by MRI. The four patients studied came due to low back pain. The MRI images demonstrated the presence of a cystic lesion located in the medullary cone, with a well defined contour and one that presented no type of enhancement in the study performed with gadolinium. In three cases, we had a MRI control at two years and the images had not varied. We have no control for the fourth cases of more recent diagnosis. The terminal ventricle is an ependymoma cavity located in the medullary cone. It can present a dilation and it is necessary to know its existence, typical location and signal characteristics to be able to diagnose and differentiate it from cystic tumors in this localization, that can present a similar appearance in the MRI. (Author) 7 refs.

  6. Desulphurisation of coal pyrolysis and magnetic separation. Desulfuracion de carbones mediante pirolisis y separacion magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz, J.C.; Ayala, N.; Ibarra, J.V.; Moliner, R.; Miranda, J.L.; Vazquez, A. (CENIM, Madrid (Spain))

    1991-07-01

    The desulphurisation of coal intended for use inthermal power stations is a priority issue in the national strategy for the reduction of acid rain. This article studies the feasibility of eliminating pyritic sulphur from coal by physical methods using high intensity pyrolysis and magnetic separation. 6 refs., 9 figs., 4 tabs.

  7. Lower hybrid resonance plasma heating in the LISA machine. Aquecimento do plasma na ressonancia hibrida inferior na maquina LISA

    Energy Technology Data Exchange (ETDEWEB)

    Silva, J C.X. da; Cunha Rapozo, C da [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Inst. de Fisica

    1988-10-01

    Plasma of helium was obtained using microwave source of f[sub RF] = 2.45 GHz and P[sub RF] = 800 W. Temperature and electron density were investigated for plasma excited by radiofrequency of f = 30 MHz and P[sub RF] = 0.1 kw. (M.C.K.).

  8. Partial resonance minimization effects on transformers benches; Minimizacao dos efeitos da ressonancia parcial de banco de transformadores

    Energy Technology Data Exchange (ETDEWEB)

    Contente, A D; Rodrigues, C G; Kataoka, D; Silveira, E; Oliveira, Junior, G F.B. de; Gonzaga, J L [Companhia Energetica de Sao Paulo, SP (Brazil)

    1992-12-31

    The problem caused by partial resonance effect in the phase regulation winding in Taubate substation transformer bench is discussed. Studies and decisions taken to use capacitors on tertiary winding of the transformers set, are presented. Damages caused by resonance on transformer contacts, studies and assays made to solve the problem, resonance frequency measured, and capacitors values calculation, are also shown 3 refs., 3 figs., 2 tabs.

  9. Comparative study between different nitrosyls hemoproteins using electron paramagnetic resonance; Estudo comparativo entre diferentes nitrosil hemoproteinas por ressonancia paramagnetica eletronica

    Energy Technology Data Exchange (ETDEWEB)

    Caracelli, Ignez

    1988-12-31

    Using the Electron Paramagnetic Resonance (EPR) technique, the properties of several nitrosyl hemoproteins were investigated as a function of temperature, pH and nitric oxide (NO) concentration. (author). 59 refs., 53 figs., 6 tabs.

  10. Nuclear quadrupole resonance applied for arsenic oxide study; Estudo do oxido de arsenio atraves de ressonancia quadrupolar nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Correia, J A.S.

    1991-04-01

    The objectives of this study are mounting a pulsed Nuclear Quadrupole Resonance (NQR) building a flow cryostat capable of varying the temperature continuously from 77 K to 340 K and using the spectrometer and the cryostat to study the polycrystalline arsenic oxide. The spin-lattice relaxation time (T{sub 1}), the spin-spin relaxation time (T{sub 2}) and the resonance frequency are obtained as a function of temperature. These data are obtained in 77 to 330 K interval. The relaxation times are obtained using the spin echo technique. The spin echo phenomenon is due to refocusing spins, when a 180{sup 0} C pulse is applied after a 90{sup 0} C pulse. The spin-lattice relaxation time is obtained using the plot of echo amplitude versus the repetition time. The spin-spin relaxation time is obtained using the plot of echo amplitude versus the separation between the 90{sup 0} C - 180{sup 0} C pulses. The theory developed by Bayer is used to explain the spin-lattice relaxation time and the frequency temperature dependence. The spin-spin relaxation time is discussed using the Bloch equations. (author).

  11. Resonance integral analytical calculation considering shadowing effect. Calculo analitico da integral de ressonancia considerando o efeito de sombreamento

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, M A.M. [NUCLEN, Rio de Janeiro, RJ (Brazil); Martinez, A S [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia

    1990-01-01

    It is presented a method for the Resonance Integral Calculation in the fuel and moderator regions, including the shadowing effect. This effect appears due to the presence of several fuel rods in a infinite moderator region. The method is based on the approximations to the J ({xi}, {beta}) function and theirs partial derivatives in relation to {beta}. The dependence of the Resonance Integral in the J ({xi}, {beta}) comes from the rational approximation to the neutron escape probability. The final results were obtained in a very simple and fast way, and they show the good accuracy of the method. (author).

  12. Decay of giant resonance E2 isoscalar in heavy nuclei. Decaimento da ressonancia gigante E2 isoescalar em nucleos pesados

    Energy Technology Data Exchange (ETDEWEB)

    Herdade, S B [Sao Paulo Univ., SP (Brazil). Inst. de Fisica

    1980-01-01

    In this work, it is made a study of the giant resonance E2 isoscalar, in heavy nuclei. Fission probabilities for this resonance were determined by various authors, in different experiments, for {sup 238}U. (A.C.A.S.).

  13. Quantitative analysis of water heavy by NMR spectroscopy; aplicacion de la resonancia magnetica nuclear al analisis cuantitativo de agua pesada

    Energy Technology Data Exchange (ETDEWEB)

    Gomez Gil, V

    1975-07-01

    Nuclear Magnetic Resonance has been applied to a wide variety of quantitative problems. A typical example has been the determination of isotopic composition. In this paper two different analytical methods for the determination of water in deuterium oxide are described. The first one, employs acetonitril as an internal standard compound and in the second one calibration curve of signal integral curve versus amount of D{sub 2}O is constructed. Both methods give results comparable to those of mass spectrometry of IR spectroscopy. (Author) 5 refs.

  14. Magnetic shape memory alloys: Basic properties and applications; Aleaciones magneticas con memoria de forma: propiedades basicas y aplicaciones

    Energy Technology Data Exchange (ETDEWEB)

    Barandiaran, J. M.; Lazpita, P.; Guiterrez, J.; Garcia Arribas, A.; Feuchtwanger, J.; Asua, E.; Etxebarria, V.; Chernenko, V. A.

    2010-07-01

    These materials can undergo large deformations when subjected to external stresses when they are in the phase low temperature due to the super elasticity property. Super elasticity properties and memory effect form derived from the martensitic transformation, which thermoelastic transformation is involved in effort as well as temperature. The transformation autoacomodante called for a single crystal phase austenite gives rise to several crystals spontaneously martensite with their axes oriented in different directions to accommodate the deformation of the network. These crystals called variant or twins and their movement and change orientation gives rise to the super elasticity. (Author) 3 refs.

  15. Blood-labyrinth barrier changes. MR assessment; Alterazioni della barriera emato-labirintica. Valutazioni mediante Risonanza Magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Manfre' , L.; Bencivinni, F.; Caronia, A.; Angileri, T.; Manasia, G.; De Maria, M. [Palermo Univ., Palermo (Italy). Ist. di Radiologia P. Cignolini

    1999-12-01

    Recent progress in Magnetic Resonance Imaging (MRI), with contrast-enhanced and steady-state sequences, allows fine depiction of labyrinth abnormalities related to neoplastic, inflammatory, ischemic, degenerative or traumatic disorders. The case of 488 patients with sensorineural hearing loss, vertigo or dizziness, but normal CT findings, was examined to evaluate MR capabilities in showing labyrinth conditions. In the period between January 1994 and May 1998, they were all submitted to CT. 68 of them, with normal CT findings, were also examined with MRI, which was performed using quadrature with MRI, which was performed using quadrature head or surface coils and a single dose (0.1. mmol/kg) Gd-DTPA administration. Conventional T1 and T2 high resolution SE images were acquired. The labyrinth was studied of 52 patients with normal CT findings and no abnormalities in the cerebello-pontine angle or internal auditory canal. 14 of 52 patients (27%) exhibited labyrinth enhancement from tumor (5%), hemorrhage (3%), infection (15%), surgical (2%) or radiosurgical (2%) procedures. GRASS (Gradient Recalled At Steady-State) sequences allowed differentiation of mass lesions (e.g. tumors, clots) from other conditions. In inflammatory conditions, enhancement is not always diffuse, as expected, but may be focal. Spontaneous hemorrhages can account for labyrinth enhancement. In neoplastic conditions, enhancement may persist for as many as 6 months, and a mass effect against labyrinthine fluids may appear on GRASS images. Although there are no reports on labyrinthine degeneration after radiation therapy, one of the patients submitted to irradiation 7 years previously, had focal bilateral cochlear enhancement, which suggested a correlation with previous treatment. [Italian] Il perfezionamento della tecnica RM, grazie all'uso del mdc paramagnetico e di sequenze sensibili al flusso labirintico, ha rivoluzionato il ruolo della metodica nello studio dell'orecchio interno, consentendo la diagnosi di malattie labirintiche di natura flogistica, neoplastica, ischemica, traumatica, iatrogena o degenerativa. Scopo del lavoro e' stato quello di valutare l'uso della metodica RM contrastografica nei pazienti affetti da disturbi clinici di origine labirintica. Nel periodo compreso tra gennaio 1994 e maggio 1998, 488 pazienti affetti da sintomatologia labirintica (ipoacusia neurosensoriale, vertigini e/o acufeni) sono stati sottoposti a TC: di questi, 68 con quadro normale sono stati sottoposti a studio mediante RM e sequenze Spin-Echo T1- e T2 pesate, prima e dopo somministrazione di mdc. L'indagine e' stata ultimata dal bilancio con scansioni in tecnica steady-state per lo studio dei fluidi endolabirintici. In 14/52 (26%) pazienti e' stata dimostrata accentuazione delle componenti dell'orecchio interno, dipendente da lesioni espansive (5%), emorragiche (3%), flogistiche (15%), chirurgiche (2%), o degenerative (2%). Il comportamento delle lesioni espansive alla sequenza in tecnica di steady-state e' differito da quello rilevato nelle lesioni con altra natura. Tra le cause piu' frequenti si e' rilevata la presenza di presunti tumori intralabirintici: l'effetto massa della lesione e' tradito dall'immagine di minus nelle immagini in RM nella sequenza steady-state per il blocco del flusso endolabirintico, a differenza di quanto rilevato nella flogosi labirintica. Un'accentuazione fisiologica di componenti isolate del labirinto membranoso e' stata rilevata anche nel monitoraggio di paziente sottoposta a exeresi di schwannoma dell'ottavo paio di nervi cranici, e in seguito a pregressa radioterapia effettuata per adenocarcinoma rinofaringeo.

  16. STUDIO DELLE AREE VISIVE IN PAZIENTI CON NEUROFIBROMATOSI DI TIPO 1 E GLIOMA DELLE VIE OTTICHE MEDIANTE RISONANZA MAGNETICA FUNZIONALE

    OpenAIRE

    Lucchetta, Marta

    2015-01-01

    Neurofibromatosis 1 (NF1) is an autosomal dominant condition characterized by neuro-cutaneous involvement and a predisposition to tumour development. The most common NF1-associated central nervous system tumour is optic pathway glioma (OPG), affecting about 15% of NF1 patients and characterized by an unpredictable evolution with no clear prognostic factors identified so far. Resting-state fMRI has recently emerged as a powerful tool for functional brain analysis, allowing the examination ...

  17. Pituitary disease in childhood: utility of magnetic resonance; Patologia hipofisaria en la edad pediatrica: unidad de la resonance magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Vela, A. C.; Oleaga, L.; Ibanez, A. M.; Campo, M.; Grande, D. [Hospital de Basurto. Bilbao (Spain)

    2000-07-01

    To assess the utility of magnetic resonance (MR) imaging in the study of pediatric patients with clinical suspicion of pituitary disease. We studied 18 patients aged 7 to 18 years.Fifteen had hormonal disturbances, two presented amenorrhea and 1 complained of headache, fever and symptoms of polyuria and polydipsia. All the patients were examined using a Siemens SP 42 1-Tesla MRI scanner. Sagittal and coronal T1-weighted spin-echo images were obtained; in addition T2-weighted spin-echo or fast spin-echo imaging was performed in ten cases and intravenous gadolinium was administered in nine. We found 9 patients with hypothalamic-pituitary dysgenesis, 2 with germinoma, 2 cases of pituitary hemosiderosis in patients with thalassemia, 2 cases of microadenoma, one abscess, one case of idiopathic central diabetes insipidus and one of Langerhans cell histiocytosis. MR enabled us to assess pituitary structural alterations in children with hypothalamic-pituitary hormone deficiencies. In our series of patients, hypothalamic-pituitary dysgenesiss was the most frequent cause of adenohypophyseal deficiencies, and most cases of central diabetes insipidus were secondary to masses in the sellar and suprasellar region. In patients with thalassemia, T2-weighted MR images showed the amount of iron deposited in adenophypophysis. Gadolinium-enhanced studies were useful in the study of masses and when the presence of microadenoma was suspected. (Author) 26 refs.

  18. Magnetic resonance arthrography in chronic wrist pain. Artografia con Risonanza Magnetica (arto-RM) nelle malattie dolorose croniche del polso

    Energy Technology Data Exchange (ETDEWEB)

    Valeri, G.; Ferrara, C. (Ancona Univ. (Italy). Cattedra di Radiologia); Carloni, S. (Ancona Univ. (Italy). Cattedra di Ortopedia) (and others)

    The purpose of this paper is to investigate the clinical role of Magnetic Resonance Arthrography (MRA) of the wrist in subjects with chronic pain. Thirty-five patients complaining of wrist pain for more than 6 months were submitted to MRI an MRA. All patients received and intra-articular injection of 2-10 mL of a 10 mmol saline solution of Gd-DPTA. The overall diagnostic accuracy rates of MRI and MRA were 40% and 81% respectively, with sensitivity and specificity of 63% and 39% (MRI) and of 82% and 79% (MRA). The conclusion is that compared with MRI, MRA can be considered a useful tool for the visualization of interosseus carpal ligaments and of the triangular fibrocartilage complex. MRA also helps detect injuries in these structures.

  19. TRATAMIENTO MÉDICO Y NUTRICIONAL EN UN PACIENTE CON ENCEFALOPATIA HEPATICA DE POSIBLE ORIGEN PORTOCAVAL

    Directory of Open Access Journals (Sweden)

    Sandra Acevedo T

    2008-08-01

    Full Text Available This article describes a case of a 3 month old female miniature schnauzer that exhibited excessive salivation, ataxia and disorientation after consuming puppy food. The physical examination revealed delayed growth for the puppy’s age, an altered mental status, and a behavior of pushing its head against the walls. Hematological tests revealed a five-fold increase in ALT and a three-fold increase in FA. Abdominal X-rays revealed microhepatica, confirmed by ultrasound. Portosistemic anastomosis was the presumptive diagnosis. Medical and dietary treatment resulted in a favorable clinical evolution.

  20. Avaliacao da Relacao entre a Doenca Hepatica Nao Gordurosa e CAD utilizando TCMF

    Directory of Open Access Journals (Sweden)

    Duran Efe

    2014-01-01

    Full Text Available Fundamento: Alguns fatores de risco para a aterosclerose são acompanhados pela doença hepática gordurosa não alcoólica (DHGNA. Desejamos usar a tomografia computadorizada multi-fatias (TCMF como a técnica para encontrar relação entre a DHGNA e a doença arterial coronariana (DAC. Objetivo: A relação entre a DHGNA e a DAC foi investigada através de TCMF. Métodos: Um total de 372 indivíduos com ou sem sintomas cardíacos, que foram submetidos à angiografia por TCMF, foram incluídos no estudo. Os pacientes foram divididos em dois grupos, de acordo com a presença da DHGNA. Os segmentos arteriais coronarianos foram avaliados visualmente via angiografia por TCMF. Com base no grau de estenose arterial coronariana, aqueles com placas ausentes ou mínimas foram considerados como normais, enquanto aqueles que apresentavam estenose de menos do que 50% e no mínimo uma placa foram considerados como portadores da doença arterial coronariana não obstrutiva (não-obsDAC. Os pacientes que apresentaram no mínimo uma placa e estenose arterial coronariana de 50% ou mais foram considerados como portadores de doença arterial coronariana obstrutiva (obsDAC. A DHGNA foi determinada de acordo com o protocolo de TCMF, utilizando a densidade hepática. Resultados: De acordo com a densidade hepática, o número de pacientes com doença hepática gordurosa não alcoólica (grupo 1 foi de 204 (149 homens, 54,8% e com fígado normal (grupos 2 foi de 168 (95 homens, 45.2%. Houve 50 (24,5% não-obsDAC e 57 (27,9% casos de obsDAC no Grupo 1, e 39 (23,2% não-obsDAC e 23 (13,7% casos de obsDAC no Grupo 2. Conclusões: O presente estudo utilizando TCMF demonstrou que a frequência da doença arterial coronariana em pacientes com NAFDL foi significativamente superior do que nos pacientes em NAFDL.

  1. Studies on Colombian Cryptogams IV. A catalogue of the Hepaticae of Colombia

    NARCIS (Netherlands)

    Gradstein, S.R.; Hekking, W.H.A.

    1979-01-01

    □ The main purpose of this catalogue is to provide a complete listing of the species of liverworts hitherto known from Colombia and to summarize our present knowledge of species distribution within the country. It was prepared parallel to a catalogue of the mosses (Musci), which is being published

  2. Nueve casos del sindrome HELLP (hemolisis, enzimas hepaticas Elevadas y plaquetopenia

    Directory of Open Access Journals (Sweden)

    M. F. Capellino

    2003-10-01

    Full Text Available El síndrome HELLP (hemólisis, enzimas hepáticas elevadas y plaquetopenia descripto por Weinstein en 1982, se asocia a alta morbi-mortalidad materna y perinatal. Se evaluaron retrospectivamente las pacientes que presentaron síndrome HELLP entre marzo 1998 y marzo 2001 en el Hospital Privado de Córdoba. Se identificaron nueve pacientes con Síndrome HELLP (incidencia 2.3‰. La edad media de las pacientes fue 24.5 años (15-36 con una edad gestacional media de 34.5 semanas (29-40. Cinco pacientes fueron nulíparas. El síntoma principal fue epigastralgia (77.7%. El parto fue por cesárea en siete pacientes y tres tuvieron síndrome HELLP post-parto. Tres pacientes presentaron hipertensión de difícil manejo, una eclampsia y otra coagulación intravascular diseminada, insuficiencia renal aguda y muerte. Tres requirieron transfusión de derivados sanguíneos y el promedio de internación fue 4.4 días. Seis (75% neonatos fueron pretérmino. El peso medio de nacimiento fue 2030 gramos (736-3200. Cuatro recién nacidos tuvieron un score de Apgar menor de 7 al minuto y todos mayor de 7 a los cinco minutos. Tres neonatos presentaron trastornos alimentarios, uno hipoglucemia y otro enfermedad de membrana hialina, ductus arterioso permeable, sepsis y plaquetopenia. Debido a la morbi-mortalidad materna y perinatal el síndrome HELLP requiere atención en un centro de alta complejidad mediante un equipo multidisciplinario.HELLP syndrome (Hemolysis, Elevated Liver Enzymes and Low Platelets was described by Weinstein in 1982. It has a high maternal and perinatal morbi-mortality rate. We undertook this study to evaluate perinatal outcome in patients with HELLP syndrome. Patients with HELLP syndrome were identified in a retrospective study between March 1998 and March 2001 at the Hospital Privado de Córdoba. Maternal and neonatal variables were analized. Nine patients with HELLP syndrome were identified (incidence 2.3‰. Mean maternal age was 24.5 (15-36 years. Five patients were nuliparous. The mean gestacional age was 34.5 weeks (29-40. The main symptom was epigastric pain (77.7%. In seven women delivery was made by cesarean section. Three patients had postpartum HELLP syndrome. Three patients had difficult control hypertension; one had eclampsia and another one had disseminated intravascular coagulopathy with acute renal failure and died. Three women needed blood products transfusions. The average admission time was 4.4 days. There were six preterm infants (75%. Mean birth weight was 2030 g (736-3200. Four neonates had Apgar score 7 at 5 minutes. Three neonates had alimentary disorders, one had hypoglucemia and another hyaline membrane disease, patent ductus arteriosus, sepsis and thrombocytopenia. The high maternal and perinatal morbi-mortality of HELLP syndrome requires management in a centre where intensive maternal and neonatal care are available.

  3. Falencia hepatica aguda en neonato a termino despues de la ingestion de dosis repetidas de paracetamol

    OpenAIRE

    Bucaretchi, Fabio; Fernandes, Carla Borrasca; Branco, Maira Migliari; Capitani, Eduardo Mello De; Hyslop, Stephen; Caldas, Jamil Pedro S.; Moreno, Carolina Araujo; Porta, Gilda

    2014-01-01

    Objective:Severe hepatotoxicity caused by paracetamol is rare in neonates. We report a case of paracetamol-induced acute liver failure in a term neonate.Case description:A 26-day-old boy was admitted with intestinal bleeding, shock signs, slight liver enlargement, coagulopathy, metabolic acidosis (pH=7.21; bicarbonate: 7.1mEq/L), hypoglycemia (18mg/dL), increased serum aminotransferase activity (AST=4,039IU/L; ALT=1,087IU/L) and hyperbilirubinemia (total: 9.57mg/dL; direct: 6.18mg/dL) after r...

  4. Evaluation of cases with Fasciola hepatica infection: experience in 6 children

    Directory of Open Access Journals (Sweden)

    Hasan Tezer

    2013-06-01

    Full Text Available Objective: To evaluate 6 pediatric cases diagnosed as fascioliasis. Methods: Patients diagnosed as fascioliasis are retrospectively interpreted. Results: Herein 6 pediatric cases having a mean age of (12.7±3.1 years with different complaints including fever, weakness, paleness, sweating, cough, headache, rectal bleeding, abdominal pain, and abdominal distension admitting to our hospital are evaluated. Conclusions: Diagnosis of fascioliasis needs a high index of suspicion supported by stool microscopy, serology, imaging procedures, and in difficult cases even liver biopsy. Triclabendazole resistance should be kept in mind in unresponsive cases.

  5. Electron spin resonance and its application to heat treated carbonaceous materials; A ressonancia de spin eletronico e sua aplicacao aos materiais carbonosos tratados termicamente

    Energy Technology Data Exchange (ETDEWEB)

    Emmerich, Francisco Guilherme [Espirito Santo Univ., Vitoria, ES (Brazil). Laboratorio de Materiais Carbonosos e Plasma Termico

    1994-12-31

    This work presents the basic characteristics of the electron spin resonance technique, also called paramagnetic resonance, being discussed its application to heat treated carbonaceous materials. In the low heat treatment temperature (HTT) range (below 700 deg C) the organic free radical are the predominant unpaired spin center, which play a key role in the process of carbonization and meso phase formation. At higher temperatures, it is possible to make correlations between the low H T T range and the high HTT range (above 130 deg C), where the predominant unpaired spin center are the free charge carriers (free electrons) of the graphite like crystallites of the material, which are formed by the carbonization process. (author) 10 refs., 3 figs.

  6. Study of conditions of iron-resonance in the transformers switching - application examples; Estudo de condicoes de ferro-ressonancia na manobra de transformadores. Exemplos de aplicacao

    Energy Technology Data Exchange (ETDEWEB)

    Portela, C; Sondermann, M A; Teixeira, J [ELETROSUL, Curitiba, PR (Brazil)

    1988-12-31

    This work identifies the iron-resonance conditions, in the switching of transformer powering from a long line, and an analysis in the frequency-time domain, with representation in detail of the saturation and hysteresis, so that we can to dimension the basic corrective instruments and to determine the correspondent parameters. Besides, it presents an hybrid analysis with more details, used for verification and confirmation of the previous processes 6 refs., 7 figs.

  7. Fano resonance in the absorption of two photons in crystal defects. Ressonancia de Fano na absorcao de dois fotons em defeitos cristalinos

    Energy Technology Data Exchange (ETDEWEB)

    Souza, G P

    1984-01-01

    The absorption of two photons in the electronic transition within the 4f/sup 7/ configuration which is superposed to the vibronic structure of the 4f/sup 7/ - 4f/sup 6/5d transition, is studied. The line shape with the Fano's formalism is described. The study was carried out on Eu/sup +2/ doped CaF/sub 2/. The experimental apparatus was built and the results obtained are in good agreement with the theoretical predictions.

  8. Resonance analysis of the transformers winding owing of system transients; Analise de ressonancia de parte de enrolamentos de transformadores devido a transitorio no sistema

    Energy Technology Data Exchange (ETDEWEB)

    Alves, M F; Ribeiro, E E [MF Consultoria Ltda (Brazil); Aguiar Campos, W de [Companhia Energetica de Minas Gerais (CEMIG), Belo Horizonte, MG (Brazil)

    1992-12-31

    An analysis of the damage on power transformer winding during occurrence of electrical transients in substations, its causes and an analysis of its occurrence situations are presented. Results of studies in the substation Ouro Preto 2, made by CEMIG (Companhia Energetica de Minas Gerais) aiming an analysis of the possibility to occur damage in transformers winding are also reported 6 refs., 8 figs., 1 tab.

  9. Subsynchronous resonance - identification of the resonance modes in systems with series compensation; Ressonancia subsincrona - identificacao dos modos ressonantes em sistemas dotados de compensacao-serie

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, J J [Brasilia Univ., DF (Brazil). Dept. de Engenharia Eletrica; Camargo, B C.C. [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Engenharia Eletrica

    1988-12-31

    This work shows the phenomenon and mechanism of the subsynchronous resonance, as well as it presents the modelling and simulation of a electromechanics system to verify the adequacy of the use of series capacitor in systems that has turbogenerators 4 refs., 7 figs., 3 tabs.

  10. Electronic Paramagnetic Resonance of irradiated nails: challenges for a dosimetry in radiation accidents; Ressonancia Paramagnetica Eletronica de unhas irradiadas: desafios para uma dosimetria em acidentes radiologicos

    Energy Technology Data Exchange (ETDEWEB)

    Giannoni, Ricardo A., E-mail: giannoni@ird.gov.br [Instituto de Radioprotecao e Dosimetria, (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Rodrigues Junior, Orlando [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2014-07-01

    The purpose of this work is to characterize samples of human nails exposed to high doses of radiation, applying the technique of Electron Paramagnetic Resonance (EPR). The objective is to establish a dose response study that allow determine the absorbed dose by exposed individuals in situations of radiological accidents, in a retrospective form. Samples of human nails were collected and afterward irradiated with gamma radiation, and received dose of 20 Gy. The EPR measurement performed on the samples, before irradiation, permitted the signal identification of the components associated with effects caused by the mechanical stress during the fingernail cutting, the so-called mechanically induced signal (MIS). After the irradiation, different species of free radicals were identified, the so-called radiation induced signal (RIS). (author)

  11. Electronic paramagnetic resonance (EPR) of spices treated by gamma irradiation; Ressonancia paramagnetica eletronica (RPE) aplicada a analise de especiarias irradiadas (com radiacao gama)

    Energy Technology Data Exchange (ETDEWEB)

    Leal, Alexandre Soares; Rodrigues, Rogerio Rivail, E-mail: asleal@cdtn.b [Centro de Desenvolvimento de Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Serv. de Reator e Irradiacoes; Krambrock, Klaus; Guedes, Kassilio [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil)

    2004-07-15

    The treatment of food by ionizing radiation is a method that has been increased in many countries in substitution for the use of chemical products. The knowledge of safe and reliable techniques of detection of irradiated food is a factor that can contribute to the largest acceptance for the consuming market. This work presents the electron paramagnetic resonance (EPR) as method of detection of the irradiated spices rosemary and cilantro. The obtained results indicate that EPR can be used satisfactorily for that group of victuals in the identification of irradiated species and in the determination of the received dose. (author)

  12. Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods

    DEFF Research Database (Denmark)

    Takeuchi-Storm, Nao; Denwood, Matthew; Hansen, Tina Vicky Alstrup

    2017-01-01

    ) as a tool for diagnosis in Danish dairy cattle farms was assessed. This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola...

  13. Fasciola hepatica vaccine: we may not be there yet but we're on the right road.

    Science.gov (United States)

    Molina-Hernández, Verónica; Mulcahy, Grace; Pérez, Jose; Martínez-Moreno, Álvaro; Donnelly, Sheila; O'Neill, Sandra M; Dalton, John P; Cwiklinski, Krystyna

    2015-02-28

    Major advances have been made in identifying potential vaccine molecules for the control of fasciolosis in livestock but we have yet to reach the level of efficacy required for commercialisation. The pathogenesis of fasciolosis is associated with liver damage that is inflicted by migrating and feeding immature flukes as well as host inflammatory immune responses to parasite-secreted molecules and tissue damage alarm signals. Immune suppression/modulation by the parasites prevents the development of protective immune responses as evidenced by the lack of immunity observed in naturally and experimentally infected animals. In our opinion, future efforts need to focus on understanding how parasites invade and penetrate the tissues of their hosts and how they potentiate and control the ensuing immune responses, particularly in the first days of infection. Emerging 'omics' data employed in an unbiased approach are helping us understand liver fluke biology and, in parallel with new immunological data, to identify molecules that are essential to parasite development and accessible to vaccine-induced immune responses. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  14. Studies on Colombian cryptogams. IIA. Hepaticae – Oil body structure and ecological distribution of selected species of tropical Andean Jungermanniales

    NARCIS (Netherlands)

    Gradstein, S.R.; Cleef, A.M.; Fulford, M.H.

    1977-01-01

    This paper is the second (Florschütz & Florschütz-de Waard 1974) in the series of reports on cryptogams of Colombia, especially the high Andean bryophytes and lichens, in the framework of recent phytosociological and ecological studies in the area by A. M. Cleef and T. van der Hammen (Amsterdam) and

  15. Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods

    DEFF Research Database (Denmark)

    Takeuchi-Storm, Nao; Denwood, Matthew; Hansen, Tina Vicky Alstrup

    2017-01-01

    ) as a tool for diagnosis in Danish dairy cattle farms was assessed. This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola...... on wet pastures, dry cows grazing on wet pastures, herd size, breed and concurrent beef cattle production were identified as risk factors associated with being classified as a case farm. With the categorised BTM ELISA result as the response variable, heifers grazing on wet pastures, dry cows grazing...... on wet pastures, and purchase of cows were identified as risk factors. Within the case and control groups, 74.8 and 12.7% of farms were positive for fasciolosis on BTM ELISA, respectively. The differences are likely to be related to the detection limit of the farm-level prevalence by the BTM ELISA test...

  16. Vasculitis secundaria a infección por Fasciola hepática Secondary vasculitis to infection by Fasciola hepatica

    Directory of Open Access Journals (Sweden)

    Germán Málaga

    2012-09-01

    Full Text Available Se presenta el caso de un paciente varón de 38 años, procedente de una zona endémica para fascioliasis hepática en el Perú. Fue hospitalizado por presentar un cuadro de cuatro semanas de evolución; con fiebre, mialgias intensas, lesiones eritematosas y dolorosas en las regiones de extensión de las extremidades. La electromiografía y la velocidad de conducción nerviosa mostraron una miopatía inflamatoria global. La biopsia de piel evidenció una vasculitis de tipo poliarteritis nodosa. En el proceso de evaluación previa a la terapia inmunosupresora, se hallaron huevos de Fasciola hepática en el examen coproparasitológico. El diagnóstico de fascioliasis se confirmó con fas2-ELISA: 0,46 (VN There is a case of a 38 year-old male patient coming from an area where hepatic fascioliasis is endemic in Peru. He was hospitalized because he showed 4 weeks of symptoms like fever, intense myalgias, erythematous and painful injuries on limb extensions. The electromyography and nerve conduction velocity showed a global inflammatory myopathy. A skin biopsy showed polyarteritis nodosa-type vasculitis. During the evaluation process prior to the immunosuppressive therapy, hepatic Fasciola eggs were found in the parasitological examination of stools. The fascioliasis diagnosis was confirmed by fas2-ELISA: 0.46 (VN <0.20. Clinical symptoms started to subside after treatment with ticlabendazol. Contact with the patient was maintained for a year and there was no evidence of disease recurrence, and he was asymptomatic

  17. Nano-magnetite coated with gold: alternative oncological therapy with magnetic hyperthermia; Nanomagnetita recubierta de oro: terapia oncologica alternativa con hipertermia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Cordova F, T.; Jimenez G, O.; Basurto I, G. [Universidad de Guanajuato, Campus Leon, Division de Ciencias e Ingenierias, Loma del Bosque 103, Lomas del Campestre, 37150 Leon, Guanajuato (Mexico); Martinez E, J. C., E-mail: theo@fisica.ugto.mx [IPN, Unidad Profesional Interdisciplinaria de Ingenieria Campus Guanajuato, Av. Mineral de Valenciana 200, Industrial Puerto Interior, 36275 Silao de la Victoria, Guanajuato (Mexico)

    2017-10-15

    Localized hyperthermia performed through the use of nanoparticles is one of the most promising procedures for the cancer treatment. In this work, the synthesis of magnetite nanoparticles (Fe{sub 2}O{sub 3}) was carried out using the thermal decomposition method. Subsequently, these nanoparticles were coated with gold and suspended in aqueous phase. As a result, nanoparticles capable of being heated by the application of an alternating magnetic field or through the use of infrared radiation were obtained. As an additional feature, these nanoparticles are biocompatible thanks to their golden coating. The synthesized nanoparticles can be functionalized by the conjugation of a molecule (aptamer, antibody, peptide, etc.) whose target is a cancer cell in order to adhere to it the nanoparticle-marker complex, to subsequently carry out a heating with the objective of induce cell death. In conclusion, the synthesized nanoparticles allow providing an alternative treatment for cancer through the use of localized hyperthermia, either using magnetic or infrared heating. (Author)

  18. Preparation of microspheres containing methyl methacrylate (MMA) with magnetic nanoparticles; Preparacao de microesferas contendo metacrilato de metila (PMMA) com nanoparticulas magneticas

    Energy Technology Data Exchange (ETDEWEB)

    Feuser, P.E.; Souza, M.N. de, E-mail: paulofeuser@hotmail.co, E-mail: nele@eq.ufrj.b [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Dept. de Engenharia Quimica

    2010-07-01

    Magnetic nanoparticles have found many technological applications and has been intensively studied due to its special magnetic properties. In most biomedical applications, microspheres containing magnetic nanoparticles is used as a vehicle for transporting drugs, presenting several advantages when compared to other conventional methods. PMMA is a polymer which has biocompatibility and can be used for the encapsulation of magnetic nanoparticles, showing a great degree of saturation magnetization. PMMA microparticles containing magnetic nanoparticles were prepared by suspension polymerization. Polymers containing magnetic nanoparticles were characterized by X-ray diffraction (XRD), vibrating sample magnetization, thermogravimetric analysis, optical microscopy, chromatography gel permeation, analysis of particle size - malversizer 2000 (Malvern Instruments). The average size of magnetic nanoparticles was approximately 150 {mu}m and depending on the amount of magnetic nanoparticles in the reaction medium Mw of microspheres can be altered. (author)

  19. Gadolinium Magnetic resonance with a diagnosis of Bell's facial palsy. Resonancia magnetica con gadolinio en el estudio de las paralisis de Bell

    Energy Technology Data Exchange (ETDEWEB)

    Rovira Caellas, A.; Sanchez Torres, C.; Navarrete, M.; Grive Isern, E.; Capellades Font, J.; Navarrete, M. (Hospital General Universitario Vall d' Hebron. Barcelona (Spain))

    1993-01-01

    The intratemporal pathway of the facial nerve has been prospectively studied by means of gadolinium MR in 12 patients with a diagnosis of Bell's facial palsy. All the cases presented total facial paralysis and were studied in the acute phase of the disease. With MR, the intratemporal pathway of the facial nerve has been viewed before and after the administration of a paramagnetic contrast medium, revealing uptake involving mainly the labyrinthine segment and the geniculate ganglion in every case. In no case did the MR findings influence the therapeutic approach, nor did they provide information of prognostic value. Therefore, this exploration is not considered necessary in the assessment of typical facial paralyses. The possible advantages of an MR study with contrast medium in facial paralysis specially apply to those cases with atypical clinical presentation, making it possible to establish a positive diagnosis, ruling out other lesions that may have a similar clinical presentantion. Thus, for the time being, a diagnosis of Bell's paralysis is not necessarily an exclusion diagnosis. (Author)

  20. Non Hodgkin lymphoma metastasis to the heart detected by cardiovascular magnetic resonance; Metastasis cardiaca secundaria al linfoma de Hodgkin detectada por la resonancia magnetica cardiovascular

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    Martinez, Florange; Morales, Marisela; Pedreanez, Norma [Hospital Cardiologico Infantil Latinoamericano Dr Gilberto Rodriguez Ochoa, Carcacas (Venezuela, Bolivarian Republic of); Pabon, Luz; Carrillo, Milton [Universidad Central de Venezuela (UCV/HUC), Caracas (Venezuela, Bolivarian Republic of). Instituto de Hematoncologia. Hospital Universitario; Fernandes, Juliano Lara [Universidade de Campinas (UNICAMP), SP (Brazil)

    2009-10-15

    Primary and secondary heart tumors are relatively rare occurrences but usually imply significant treatment decisions. The differential diagnosis among these tumors and other masses can sometimes be difficult and require the use of different imaging modalities to establish a confident verdict. Cardiovascular magnetic resonance CMR imaging is a very useful tool in these cases by allowing for the application of different strategies to better delineate masses, heart structures and adjacent tissues. In this case description, we present a woman with shortness of breath and a paracardiac mass showing how CMR can be applied. (author)

  1. High speed magnetically levitated transport systems: Technical and economic aspects. Sistemi di trasporto ad alta velocita' a levitazione magnetica: Considerazioni tecniche ed economiche

    Energy Technology Data Exchange (ETDEWEB)

    Martinelli, G; Morini, A [Padua Univ. (Italy)

    1992-11-01

    In assessing the current state of technology relative to high speed (400 to 500 km/h) magnetically levitated (MAGLEV) transportation systems, this paper compares the technical aspects of the two main types of suspension systems now being developed specifically for these transportation systems, i.e., attractive electromagnetic using conventional magnets, and repulsive electrodynamic using superconducting magnets. Commercialization prospects for these systems are reviewed by analyzing areas where high speed levitated trains could compete against conventional rail and air transportation systems. The technology review includes progress reports on research and developments activities taking place in Germany and Japan, as well as, descriptions of some recent applications of this technology.

  2. Endometrial carcinoma: merit of magnetic resonance in pre-surgical staging; Carcinoma de endometrio: valor de la estadificacion prequirurgica por resonancia magnetica

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    Fernandez, E.; Barrera, M. C.; Gervas, C.; Salvador, E.; Rivero, B. [Hospital Donostia. San sebastian (Spain); Sentis, M. [Corporacio Sanitaria Pare Tauli. Sabadell (Spain)

    2003-07-01

    To evaluate MR capacity in assessing deep myometrial and cervical infiltrations in cases of endometrial carcinoma. A series of 30 consecutively diagnosed endometrial cancer patients was pre-surgically evaluated by means of magnetic resonance (MR). TSE-T2 sequences with fat saturation and dynamic FFe sequence were used after gadolinium administration. A correlation with post-surgical histological stating was made. There were then determined sensitivity (S), specificity (SP), positive predictive value (PPV) and negative predictive value (NPV) for the deep myometrial infiltration and cervical invasion. Cases of overestimation and underestimation were analyzed. Values obtained for myometrium and cervix were, respectively, S of 67% and 63%, SP of 89% and 91%, PPV of 80% and 71% and NPV of 80% and 87%. Two cases each were over valued for myometrial infiltration and cervix: four cases and 3 cases, respectively, were undervalues. MR stating in cases of endometrial carcinoma is a highly reliable diagnostic technique, but it does present certain limitations. (Author) 19 refs.

  3. The inappropriate use of lumbar magnetic resonance imaging in a health service area; Utilizacion inapropiada de la resonancia magnetica lumbar en un area de salud

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    Rodriguez Recio, F. J.; Sanz, J. C.; Vera, S. [Hospital General de Soria (Spain); Peiro, S. [Fundacion de Investigacion en Servicios de Salud Valencia (Spain)

    1999-07-01

    To identify the percentage of inappropriate lumbar spine magnetic resonance imaging in the Soria Health Service, to quantify the costs and the possible association between inadequate use, the characteristics of the patient and the services requested. A descriptive study of the inappropriate use of MRI of the lumbar spine, taken from the retrospective examination, carried out by a radiologist, of the 233 MRI's requested between 1995 and 1998. For the valuation, the criteria of the American College of Radiology (ACR) and the Basque Agency for the Evaluation of Technologies (OSTEBA) were used. All the MRI's were carried out at an approved centre, the costs were calculated taken form the expenses paid by the Insalud, including the transport costs, calculated at prices applicable for the year in question. 11.7% of the studies were values as inappropriate, 2.1% debatable and the remainder adequate according to the ACR criteria, concentrating the inadequacy on studies for lumbago, that reached 80% of the inappropriate requests. The ACR and OSTEBA criteria coincided to a high degree (kappa statistics: 0.87). The expense related to the unnecessary studies was a litter higher than a million pesetas. No differences were found in the proportion of inappropriate studies according to the characteristics of the patient or the service requested, except the one already mentioned for the supposition diagnosis. Although the results of the study cannot be generalised to other environments, they suggest the possibility of a significant proportion of inappropriate use of lumbar spine MRI that could have an important repercussion on health care expenses. (Author) 11 refs.

  4. First steps in quality control equipment MRI; Primeros pasos en el control de calidad de equipos de imagen por resonancia magnetica

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    Alonso Diaz, M.; Castaneda Arronte, M. J.; Ferreiros Vazquez, N.; Sanchez Merino, G.

    2011-07-01

    The present work aims to develop the initial stages of a program of quality control of nuclear magnetic resonance equipment. Not intended to conduct a comprehensive study covering all aspects of quality control, but gather the steps are followed and the most common problems they are encountering during the development of this program. It has been claimed that the work will be helpful to others facing the same task. Have been studied the compatibility of mannequins and procedures for the various teams. Ideally, such controls should be independent of the procedures and mannequins own commercial houses.

  5. Spontaneous intracranial hypotension syndrome: magnetic resonance findings in two patients; Sindrome de hipotension intracraneal espontanea: hallazgos en resonancia magnetica en dos pacientes

    Energy Technology Data Exchange (ETDEWEB)

    Ortega, R.; Pastor, J.; Escamilla, F.; Romero, M. I. [Hospital de Traumatologia y Reabilitacion. Granada (Spain)

    1999-07-01

    The postural headache syndrome associated with a decrease in the cerebrospinal fluid (CSF) pressure is generally secondary to the CSF leakage that usually occurs after diagnostic lumbar puncture. Spontaneous intracranial hypotension can not be attributed to any known cause or previous diagnostic or therapeutic intervention. The syndrome is characterized by severe headache that is relieved by lying supine. During lumbar puncture, the CSF pressure is normally low. We present two patients in whom gadolinium-enhanced magnetic resonance imaging showed widespread thickening and enhancement of the dura mater and subdural fluid collections. (Author) 13 refs.

  6. Non-traumatic acute epidural spinal hematomas diagnosed by magnetic resonance; Hematomas espinales epidurales agudos no traumaticos: diagnostico por resonancia magnetica

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    Rovira, A.; Grive, E.; Pedraza, S.; Capellades, J.; Nos, C.; Alarcon, M.; Rovira, A. [Hospital Universitari Vall d' Hebron. Barcelona (Spain)

    2000-07-01

    The non-traumatic spinal epidural hematoma (NTSEH) is a rare entity that can be the cause of an acute spinal compression syndrome. the objective of this review is to identify the characteristics by MRI and NTSEH and to analyze the factors that influence in its prognosis. In the years 1994 and 1999, 12 patients with NTSEH have been diagnosed in our hospital, and a MRI was performed during the acute phase. the characteristics of the lesions have been analyzed by MRI, with special emphasis on the topographic data and resonance signal and the factors that can influence in the clinical prognosis of the patients. Initially, all of the patients presented pain in the cervical dorsal or interscapular site, followed by a sensitive-motor deficit picture. The MRI showed a lesion of expansive character and posterior epidural location in every case that would produce varying degrees of compression on the spinal cord. The NTSEH should be considered as one of the causes of acute spinal cord compression. The clinical association of intense cervical, dorsal or interscapular pain followed by a sensomotor deficit picture should lead to the suspicion of this entity, that would require an immediate examination with MRI to verify its diagnosis. Both the clinical manifestations as well as the characteristics observed by MRI of the NTSEH have a prognostic value and determine the therapeutic decision. (Author) 34 refs.

  7. Usefulness of magnetic resonance in the evaluation of perianal fistulas; Utilidad de la resonancia magnetica en la valoracion de las fistulas perianales

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    Campo, M.; Isusi, M.; Oleaga, L.; Grande, D. [Hospital de Basurto. Bilbao (Spain); Fernandez, G.; Tardaguila, F. [Clinica POVISA. Vigo (Spain)

    2003-07-01

    Our aim was to confirm the usefulness of magnetic resonance in the evaluation of perianal fistulas, and in conjunction with a thought anatomical review of affected areas. This would allow for the building of a proper surgical plan, which would necessarily differ according to the fistula's complexity. We studied 75 patients with perianal fistulas and performed 81 MR studies by means of axial, sagittal and coronal T1 and T2 sequences. Fistula type, degree, etiology and correlation to surgical findings were all studied. Fifty-five patients underwent surgical treatment, and 26 underwent a more conservative treatment with MR follow-up. Ninety-nine fistulas were observed and classified according to norms set down by St. James University Hospital. Of the 55 cases submitted to surgery, 46 showed concordance between the surgical report and MR, whose sensitivity was 84%. In 9 patients, there was no correlation. In the study of perianal fistulas, it is important to establish both the fistulous tract and relationship to the sphincter complex. MR permits an identification of the sprinter complex and a more precise anatomical localization of the fistulous trajectory. It is also capable of differentiating between fibrosis and abscess. Therefore, MR is an appropriate technique for the study of perianal fistulas and related surgical planning. (Author) 6 refs.

  8. Synovial hemangiomas of the knee: magnetic resonance findings in six cases; Hemangiomas sinoviales de rodilla: hallazgos de la resonancia magnetica en seis casos

    Energy Technology Data Exchange (ETDEWEB)

    Concepcion, L.; Marti-Bonmati, L. M.; Dosda, R. [Hospital Dr. Peset. Valencia (Spain); Llauger, J.; Palmer, J. [Hospital Santa Creu i Sant Pau. Barcelona (Spain); Mellado, J. M. [Inscanner, S. L. Alicante (Spain)

    1999-05-01

    The synovial hemangioma is an uncommon benign vascular tumor that is difficult to diagnose on the basis of clinical signs Moreover, it has no characteristic radiographic features. The objective of the present report was to describe the MR findings associated with synovial hemangioma of the knee. We review the clinical and MR findings in six patients, with histologically confirmed synovial hemangioma of the Knee, studied with different MR systems and techniques. Synovial hemangiomas were isointense with respect to muscle in T1-weighted images, strongly hyperintense in T2-weighted sequences and presented wavy hypointense linear images. Gadolinium administration resulted in a marked enhancement, although it was heterogeneous in two of three cases analyzed. Although the findings are not pathognomonic, the presence of an intraarticular tumor of the knee that is isointense with respect to muscle in T1 and hyperintense in T2, and shows wavy hypointense images and a marked contrast uptake, may suggest the presence of synovial hemangioma. (Author) 11 refs.

  9. Comparison between magnetic resonance cholangiopancreatography (MRCP) and endoscopic retrograde cholangiopancreatography (ERCP); Comparacion entre calongiografia por resonancia magnetica (CPRM) y colangiografia retrograda endoscopia (CPRE)

    Energy Technology Data Exchange (ETDEWEB)

    Pulpeiro, J. R.; Armesto, V. [Clinica Nosa Senora dos Ollos Grandes. Lugo (Spain); Lopez-Roses, L.; Lancho, A.; Gonzalez, A. [Hospital Xeral. Lugo (Spain)

    2000-07-01

    To assess the correlation between magnetic resonance cholangiopancreatography (MRCP) and endoscopic retrograde cholangiopancreatography (ERCP). The authors carried out a prospective study of 71 patients suspected of having biliary diseases. MRCP was performed, followed by ERCP.MRCP was done with a 1.5-T system using hypergradients, abdominal phase array coil and single-shot fast spin-echo (SSFSE) sequences with maximum signal intensity in T2-weighted images. This sequence only provides images of the fluids, similar to those obtained with ERCP. The results of ERCP were employed as a reference standard. Thirty patients were found to have stenosis (19 malignant and 11 benign). 25 had choledocholithiasis and 16 showed no abnormal findings. The correlation was complete in patients with stenosis. MRCP detected 23 cases of choledocholithiasis (ERCP was normal in the remaining two patients, but calculi were discovered on shpincterotomy). MRCP confirmed the normal condition of the bile duct in 12 of the 16 patients free of biliary disease (2 patients in whom ERCP had been normal had been diagnosed as having bile duct dilatation due to choledocholithiasis 24 hours and 10 days earlier, respectively, and two cases of presented ectasia due to fibrosis of the sphincter of Oddi showed good emptying during ERCP). MRCP using SSFSE is highly correlated with ERCP, which should be reserved for cases in which the diagnosis is in doubt or endoscopic management is necessary. (Author) 25 refs.

  10. The use of Magnetic Resonance Sounding in shallow aquifers IN THE Duero RIVER Basin; Aplicacion de sondeos de resonancia magnetica en acuiferos superficiales de la Cuenca del Duero

    Energy Technology Data Exchange (ETDEWEB)

    Uriarte Blanco, C.; Plata Torres, J. L.; Diaz-Curiel, J.; Martinez-Fernandez, J.

    2011-07-01

    To manage the water resources of a region efficiently it is vital to be aware of the dynamics and evolution of its groundwater. To this end groundwater models are used, but these models require information about the geometry and hydraulic parameters of the aquifer, which is generally quite expensive to obtain. Magnetic resonance sounding (MRS) is a non-invasive geophysical technique that allows an aquifer to be characterized. Our intention here is to assess the use of this geophysical technique to optimize the acquisition of data when preparing a hydrological model of surface aquifers in the Duero Basin. The study was undertaken in the Experimental Basin of Carrizal, within the Los Arenales aquifer in the Duero Basin. We present a detailed analysis and interpretation of the MRS results, which have provided us with information concerning the parameters needed to establish a hydrological model of the aquifer, information that may be used eventually as an input to obtain a hydrological model of the whole basin. (Author)

  11. Magnetic resonance in the diagnostic imaging study of mesial temporal sclerosis; Diagnostico por imagen con resonancia magnetica de la esclerosis temporal mesial

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    Pastor, E; Sanchez, J C; Rodriguez, I; Altuzarra, A; Machado, F [Hospital Universitario Virgen de las Nieves. Granada (Spain); Aguilar, D [Hospital Clinico Universitario San Cecilio. Granada (Spain)

    2001-07-01

    Mesial temporal sclerosis (MTS) consists of hippocampal atrophy and gliosis and is the most common cause of temporal lobe epilepsy. The objective of the authors was to establish a magnetic resonance imaging (MRI) protocol for its diagnosis. A prospective study was carried out in 72 patients with drug resistant complex partial seizures (42 women and 30 men ranging in age from 6 to 66 year: mean: 30 years). Using a 1.5-Tesla magnet, paracoronal sections were made in hippocampi for T1-weighted inversion-recovery images and volume measurements, fluid-attenuated inversion-recovery (FLAIR) and T2 relaxometry. A control group of 30 health volunteers was included in the study. MTS was considered to be indicated by the presence of atrophy and hyperintensity in hippocampi on T2-weighted images. There were no differences among the hippocampi of the healthy individuals. The confidence intervals (mean{+-} 1.96 SD) were 4169 mm''3-5911 mm''3 for volume of right side, 4097 mm''3-5940 mm''3 for volume of left side and 98-113 ms for T2 relaxation time. MTS was detected in 40 patients (55.5%): 23 cases involving the left side, 13 involving the right and 4 cases of bilateral asymmetric involvement. The 95% confidence intervals for the diagnostic validity of the results (sensitivity/specificity) were (88.8%-97.2%)/(87.6%-96.4%) for T1 volumetry, (88.8%-97.2%)(95.7%-100.3%) for FLAIR and (85.4%-96.6%)/(85.4%-96.6%) for T2 relaxometry. In 5 cases of MTS, astrophy of other extra hippocampal structures was also observed, and MTS was associated with an extra hippocampal lesion (dual pathology), especially neurona migration disorders, in 8 patients. Seventeen patients (23.5%) presented lesions without MTS (tumors, cortical dysplasias and heterotopias) and there was no MRI evidence of anomalies in 15 (21%). Twenty-five patients underwent surgical treatment: 20 with MTS (19 diagnosed according to MRI and one in whom there had been no abnormal findings), 4 with tumors and 1 with a ballooned cell cortical dysplasia. (Author) 50 refs.

  12. Magnetic resonance in the diagnostic imaging study of mesial temporal sclerosis; Diagnostico por imagen con resonancia magnetica de la esclerosis temporal mesial

    Energy Technology Data Exchange (ETDEWEB)

    Pastor, E.; Sanchez, J. C.; Rodriguez, I.; Altuzarra, A.; Machado, F. [Hospital Universitario Virgen de las Nieves. Granada (Spain); Aguilar, D. [Hospital Clinico Universitario San Cecilio. Granada (Spain)

    2001-07-01

    Mesial temporal sclerosis (MTS) consists of hippocampal atrophy and gliosis and is the most common cause of temporal lobe epilepsy. The objective of the authors was to establish a magnetic resonance imaging (MRI) protocol for its diagnosis. A prospective study was carried out in 72 patients with drug resistant complex partial seizures (42 women and 30 men ranging in age from 6 to 66 year: mean: 30 years). Using a 1.5-Tesla magnet, paracoronal sections were made in hippocampi for T1-weighted inversion-recovery images and volume measurements, fluid-attenuated inversion-recovery (FLAIR) and T2 relaxometry. A control group of 30 health volunteers was included in the study. MTS was considered to be indicated by the presence of atrophy and hyperintensity in hippocampi on T2-weighted images. There were no differences among the hippocampi of the healthy individuals. The confidence intervals (mean{+-} 1.96 SD) were 4169 mm''3-5911 mm''3 for volume of right side, 4097 mm''3-5940 mm''3 for volume of left side and 98-113 ms for T2 relaxation time. MTS was detected in 40 patients (55.5%): 23 cases involving the left side, 13 involving the right and 4 cases of bilateral asymmetric involvement. The 95% confidence intervals for the diagnostic validity of the results (sensitivity/specificity) were (88.8%-97.2%)/(87.6%-96.4%) for T1 volumetry, (88.8%-97.2%)(95.7%-100.3%) for FLAIR and (85.4%-96.6%)/(85.4%-96.6%) for T2 relaxometry. In 5 cases of MTS, astrophy of other extra hippocampal structures was also observed, and MTS was associated with an extra hippocampal lesion (dual pathology), especially neurona migration disorders, in 8 patients. Seventeen patients (23.5%) presented lesions without MTS (tumors, cortical dysplasias and heterotopias) and there was no MRI evidence of anomalies in 15 (21%). Twenty-five patients underwent surgical treatment: 20 with MTS (19 diagnosed according to MRI and one in whom there had been no abnormal findings), 4 with tumors and 1 with a ballooned cell cortical dysplasia. (Author) 50 refs.

  13. Dosimetric evaluation in mammary glands radiotherapy using expansive prosthesis with magnetic valve; Avaliacao dosimetrica de tratamentos de radioterapia de mama usando proteses expansivas com valvula magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Lizar, J.C.; Pavoni, J.F.; Baffa, O. [Universidade de Sao Paulo (USP), Ribeirao Preto (Brazil); Krutman, Y. [Rabin Medical Center (RMC), (Israel)

    2016-07-01

    This work aims to verify the influence on dose distribution that the expansive prosthesis (SILIMED/470), containing a magnetic valve, generates in radiotherapy postoperative treatment of patients who have undergone mastectomy. (author)

  14. Pathologic conditions in pregnancy; Preliminary evaluation of MR efficacy. Condizioni patologiche nella gravidanza; Valutazioni preliminari sull'efficacia della Risonanza Magnetica

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    Beomonte Zobel, B; Tella, S; Innacoli, M; D' Archivio, C; Cardone, G; Masciocchi, C; Gallucci, M; Passariello, R [L' Aquila Univ. (Italy). Cattedra di Radiologia; Cappa, F [L' Aquila Univ. (Italy). Clinica Ostetrica-Ginecologica

    1991-01-01

    Soma authors suggested that MR imaging could rapresent an effective diagnostic alternative in the study of pathologic conditions of mother and fetus during pregnancy. To verify the actual role of MR imaging, we examined 20 patients in the 2nd and 3rd trimester of gestation, after a preliminary US examination. Fifteen patients presented fetal or placental pathologies; in 4 patients the onset of the pathologic condition occurred during pregnancy; in 1 case of US diagnosis of fetal ascites, MR findings were nornal and the newborn was healty. As for placental pathologies, our series included a case of placental cyst, two hematomas between placenta and uterine wall, and two cases of partial placenta previa. As for fetal malformation, we evaluated a case of omphalocele, one of Prune-Belly syndrome, a case of femoral asimmetry, one of thanatophoric dwarfism, a case of thoracopagus twins with cardiovascular abnormalities, two fetal hydrocephali, and three cases of pyelo-ureteral stenosis. As for maternal pathologies during pregnancy, we observed a case of subserous uterine fibromyoma, one of of right hydronephrosis, one of protrusion of lumbar invertebral disk, and a large ovarian cyst. In our experience, MR imaging exhibited high sensitivity and a large field of view, which were both useful in the investigation of the different conditions occurring during pregnancy. In the evaluation of fetal and placental abnormalities, especially during the 3rd trimester, the diagnostic yieldof MR imaging suggested it as a complementary technique to US for the evaluation of fetal malformation and of intrauterine growth retardation.

  15. Automatic storing of single gamma spectra on magnetic tape. Programs LONGO, DIRE; Automatizacion del almacenamiento en cinta magnetica de espectros gamma directos. Programas LONGO, DIRE

    Energy Technology Data Exchange (ETDEWEB)

    Los Arcos Merino, J M

    1978-07-01

    The program LONGO provides the block size and the block number in a binary file on magnetic tape. It has been applied to analyse the structure of the nine-track magnetic tapes storing single or coincidence gamma spectra files, recorded in octet form by a MULTI-8 minicomputer in the Nuclear Spectrometry Laboratory of J.E.N. Then the program DIRE has been written to transform the single gamma spectra into a new FASTRAND disk file, storing the information in-36 bit words. A copy of this file is obtained on magnetic tape and the single gamma spectra are then available by standard FORTRAN V reading sentences. (Author) 3 refs.

  16. Multiple sclerosis and anterograde axonal degeneration study by magnetic resonance. Asociacion de esclerosis multiple y degeneracion Walleriana estudio por resonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Martinez Pardo, P; Capdevila Cirera, A; Sanz Marin, P M; Gili Planas, J [Centro de Resonancia Magnetica de Barcelona (Spain)

    1993-01-01

    Multiple sclerosis (MS) is a disease of the central nervous system that affects specifically the myelin. Its diagnosis by imaging techniques is, since the development of magnetic resonance (MR), relatively simple, and its occasional association with anterograde axonal degeneration (WD) has been reported. In both disorders, there is a lengthening of the T1 and T2 relaxation times. In the present report, 76 patients with MS with less than 4 plaques in the typical periventricular position were studied retrospectively, resulting in a rate of association with anterograde axonal degeneration of 8%. We consider that in spite of their same behavior in MR,MS and WD, with moreover represent completely different pathologies, are perfectly differential by MR. The S-E images with longer repetition and echo times in the axial and coronal planes have proved to be those most sensitive for this differentiation. Given that MS is specific pathology of then myelin, the axonal damages in delayed until several plaques adjacent to an axon affect it. We consider that this, added to the restriction of our study group (less than 4 plaques), is the cause of the pow percentage of the MS-WD association in our study. (Author).

  17. MR imaging in the diagnosis of the os trigonum syndrome; Il ruolo della Risonanza Magnetica nella diagnosi della sindrome dell'os trigonum

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    Tamburrini, O.; Porpiglia, H.; Barresi, D.; Bertucci, B.; Console, D. [Catanzaro Univ. Magna Grecia, Catanzaro (Italy). Radiologia, Dipt. di Medicina Sperimentale e Clinica

    1999-12-01

    In this paper is investigated the yield of Magnetic Resonance Imaging (MRI) in hind foot conditions, particularly the os trigonum syndrome, which are very difficult to diagnose clinically. 7 consecutive patients were examined. They were complaining of hind foot pain for more than 4 months (male:female=1:6; age range 16-22 years, average 18.6). Three patients practiced competitive sports and 4 ballet. It was performed conventional radiography (orthogonal projections) and then MRI with a 0.5 T superconductive unit with surface coils; MR images were acquired with T1-weighted spin-echo (SE), T2-weighted gradient-echo (GRE), and fast inversion recovery (FIR) fat-suppressed sequences with 4 mm thickness and 0 mm gap. In 2 cases the os trigonum had irregular margins with subchondral sclerosis and widened synchondrosis. In 3 patients it was found flessor hallucis longus tenosynovitis, likely caused by tendon compression and displacement within its sheath; there were neither os trigonum marrow edema nor synchondrosis widening. One patient had os trigonum hypertrophy, mild synchondrosis widening and marrow edema, in the os trigonum and the posterior aspect of talus. One patient had the os trigonum, but no signs referable to the os trigonum syndrome. In the posterior impingement syndrome, the objective is to show inflammatory changes in the posterior capsule of the ankle joint, adjacent ligaments, tendons and chondrosynovial surface. MR provides important information on soft tissues involvement, synovial reaction, chondral and subchondral bone injuries and the association of flexor hallucis longus synovitis, if present. MRI also yields detailed information for correct therapeutic approach. In conclusion, MRI appears to be the technique of choice, after conventional radiography, thanks to its non invasiveness, multi planarity, and high spatial and contrast resolution. [Italian] Scopo di questo lavoro e' quello di sottolineare il determinante contributo informativo della RM nell'ambito delle malattie dolorose del retropiede e piu' in particolare nella sindrome dell'os trigonum, in considerazione della notevole difficolta' clinica per la corretta diagnosi. Lo studio e' stato condotto su sette pazienti che lamentavano dolore al retropiede da piu' di quattro mesi (maschi/femmine=1/6), con eta' compresa tra 16 e 22 anni (eta' media 18,6 anni); 3/7 praticavano attivita' agonistica sportiva, 4/7 danza classica. In 2/7 pazienti l'os trigonum presentava margini irregolari con sclerosi subcondrale e ampliamento della sincondrosi. In 3/7 pazienti e' stata documentata tenosinovite del tendine flessore lungo dell'alluce causata verosimilmente dalla sua compressione e spostamento all'interno della propria guaina, senza ne' alterazione dell'intensita' di segnale dell'os trigonum, ne' ampliamento della sincondrosi. In 1/7 pazienti sono stati rilevati ipertrofia dell'os trigonum, modico ampliamento della sincondrosi e segni importanti di edema midollare nell'os trigonum e nel processo posteriore dell'astragalo. In 1/7 pazienti, pur in presenza di os trigonum rilevato all'esame radiologico tradizionale, l'esame RM non ha documentato alcuno dei segni da riferire a sindrome dell'os trigonum: la diagnosi definitiva e' stata tendinite achillea. La RM e' l'unica metodica che fornisce importanti informazioni sul coinvolgimento dei tessuti molli, della superficie condro-ossea e sulle reazioni sinoviali riuscendo percio' a dimostrare chiaramente l'eventuale coesistente tenosinovite del tendine flessore lungo dell'alluce ed inoltre provvedendo a fornire informazioni dettagliate per il corretto approccio terapeutico. In conclusione, nell'ambito delle malattie dolorose del retropiede da cause ossee, laddove si sospetti clinicamente la sindrome dell'os trigonum, la non invasivita' della RM, unitamente alle sue panoramicita', risoluzione spaziale e risoluzione di contrasto, ne fanno metodica d'elezione, dopo l'esame radiologico tradizionale.

  18. Brain MRI in 17 patients with ocular Behcet`s disease; Risonanza Magnetica dell`encefalo in 17 pazienti affetti da malattia di Behcet oculare

    Energy Technology Data Exchange (ETDEWEB)

    Accorinti, Massimo; Pivetti Pezzi, Paola [Rome, Univ. (Italy). Istituto di Oftalmologia; Di Biasi, Claudio; Trasimeni, Guido; Melone, Antonio; Gualdi, Gianfranco [Rome, Univ. (Italy). Unita` TC-RM I Clinica Medica

    1997-04-01

    Behcet`s disease is a chronic relapsing disorder of unknown etiology characterized by oral aphthous ulcerations, uveitis, genital ulcerations and bone lesions. A variety of other signs including polyarthritis, vascular conditions (blood vessel occlusions and aneurysms), epididymitis, gastrointestinal, pulmonary and heart lesions may also occur. Central nervous system (CNS) involvement is reported in 10-49 % of cases and it is the first symptom of the disease in 5 % of subjects. The neuro-Behcet`s syndrome may appear as a brainstem syndrome, and an organic confusional syndrome or dementia. cranial hypertension, mostly related to cerebral venus thrombosis, is also present in neuro-Behcet`s disease and its incidence is reported in up to 10 % of Behcet`s patients. MRI is reportedly the most sensitive neuroradiological approach to detect the focal lesions related to neuro-Behcet disease and several single cases or series of Behcet`s patients with neurologic sings have been examined with MRI. They used MRI to investigate CNS involvement in Behcet`s disease patients with and without previous neurologic sings. MRI was carried out on 17 patients with ocular Behcet`s disease without neurological symptoms to assess the possible subclinical involvement of the CNS. PD and T2-weighted hypersignal foci were demonstrated in parietal, frontal, subcortical and periventricular white matter in 6 subjects. Neuroradiological abnormalities were found only in patients with complete disease and with the disease diagnosed more than 10 years earlier. Even though the pathogenesis of these neuroradiological abnormalities and their correlation with Behcet`s disease remain to be clarified, their study suggests the possibility of subclinical CNS involvement in these patients, which may affect the therapeutic approach and their prognosis.

  19. Characterization of steel grit recovered from ornamental rocks waste by magnetic separation; Caracterizacao da granalha de aco recuperada do residuo de rochas ornamentais por separacao magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Junca, E.; Telles, V.B.; Rodrigues, G.F.; Oliveira, J.R. de; Tenorio, J.A.S., E-mail: eduardojunca@gmail.co [Universidade de Sao Paulo (USP), SP (Brazil). Dept. de Engenharia Metalurgica e de Materiais; Instituto Federal de Ciencia e Tecnologia do Espirito Santo (IFES), Vitoria, ES (Brazil)

    2010-07-01

    The aim of this work is characterization of steel grit recovered from ornamental rock waste by magnetic separation. The magnetic separation was realized in three steps: first, using a high intensity wet magnetic separator, which used only the remaining magnetic field of equipment. In the second step, the magnetic material obtained in the first phase was subjected to a new manual magnetic separation using rare earth magnets. In a third step, magnetic material obtained with rare earth magnets was subjected to manual magnetic separation using ferrite magnets. After the magnetic separation, the material was sent to characterization which was obtained by chemical analysis, scanning electron microscopy, X-ray diffraction and size analysis. The size analysis showed that the concentrate range from 0,5 to 563,67 {mu}m with 4 wt.% over 100 {mu}m and content metallic iron of 93 wt%. (author)

  20. Continuous partial status cause of hyperintensity in cerebral cortex in magnetic resonance imaging; Estatus parcial continuo, causa de hiperintensidad de la corteza cerebral en la resonancia nuclear magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Lopez L, Ingeborg; Gonzalez L, Daniela [Departamento de Ciencias Neurologicas Oriente. Facultad de Medicina, Universidad de Chile, Santiago (Chile); Quezada R, Patricio [Servicio de Neurologia, Hospital Salvador, Santiago (Chile); Cartier R, Luis [Departamento de Ciencias Neurologicas Oriente. Facultad de Medicina, Universidad de Chile, Santiago (Chile)

    2012-07-01

    Magnetic Resonance Imaging has demonstrated functional changes of the cerebral cortex in relation to status epilepticus, which can eventually localize the origin of the crisis. The purpose of this presentation is relevant to this condition and pretends to highlight the action of incidental situations that can modify it. We present a 29 year old woman with a neurosurgical intervention for a neuroblastoma irradiated fifteen years ago, which incidentally starts a continuous partial status epilepticus, expressed by clonies of the face and left limbs associated with functional impotence, resistant to oral therapy. Faced with the suspicion of recurrence of the tumor, a brain MRI is performed, showing hyperintensity of all neural areas the right hemisphere, with no evidence of tumor recurrence. Once submitted the status epilepticus, the hyperintensity disappeared in the hemisphere. This extensive reaction of the neural structures might be related to a permanent effect of radiation, which may have caused a mismatch functional glia, of the blood-brain barrier and interneural network.

  1. Electron paramagnetic resonance study on the ionizing radiation induced defects of the tooth enamel hydroxyapatite; Estudo por ressonancia paramagnetica eletronica de defeitos induzidos pelas radiacoes ionizantes na hidroxiapatita do esmalte dentario

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Liana Macedo de

    1995-01-01

    Hydroxyapatite is the main constituent of calcified tissues. Defects induced by ionizing radiations in this biomineral can present high stability and then, these are used as biological markers in radiological accidents, irradiated food identifying and geological and archaeological dating. In this work, paramagnetic centers induced on the enamel of the teeth by environmental ionizing radiation, are investigated by electron paramagnetic resonance (EPR). Decay thermal kinetic presents high complexity and shows the formation of different electron ligation energy centers and structures 65 refs., 40 figs., 5 tabs.

  2. Some effects that influence the measurement of a nuclear resonance by using of a crystal spectrometer. Alguns efeitos que influenciam a medida de uma ressonancia nuclear com um espctrometro de cristal

    Energy Technology Data Exchange (ETDEWEB)

    Stasiulevicius, R [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil)

    1993-10-01

    This work describes the neutron transmission experiments aimed at evaluating the thermal resonance of [sup 176] Lu by using a crystal spectrometer located next to an irradiation channel of the IEA-R1 reactor. The iridium resonance was used to calibrate the experimental data. The effects of several phenomena affecting the measurements were also accounted for. Finally, the results were compared to values from the literature. (author).

  3. Computed tomographic study of 50 patients with hypodense hepatic injuries in childhood; Estudo de 50 casos por tomografia computadorizada de lesoes hipodensas hepaticas fundamentais na infancia

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Ines Minniti Rodrigues; Alvares, Beatriz Regina; Baracat, Jamal; Martins, Daniel Lahan [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Ciencias Medicas. Dept. de Radiologia]. E-mail: iminniti@fcm.unicamp.br; Pereira, Ricardo Minniti Rodrigues [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Ciencias Medicas

    2006-03-15

    Objective: To describe the different tomographic findings in hypodense hepatic lesions in children and its differential diagnosis. Materials and methods: computed tomographic studies were obtained from 50 patients (age range: 0-16 years) with low-density liver lesions previously diagnosed by ultrasound. Images were made before and after administration of intravenous contrast medium. Image findings were analyzed and afterwards correlated with anatomopathological diagnosis. Results: forty-seven of 50 cases were confirmed, 30 by anatomopathological diagnosis. Most of then were benign lesions, hemangioma in 20%. Such lesions presented a homogeneous contrast absorption, mainly at the delayed phase, differing from malignant lesions. Metastasis was the most frequently found malignant lesion (18%). Conclusion: computed tomographic study is of great value in complementing the diagnosis of hypodense hepatic lesions in children, and must follow ultrasound diagnosis as a routine procedure. (author)

  4. Hepatic artery aneurysm simulating a lesion in the head of the pancreas: a case report; Aneurisma de arteria hepatica simulando lesao em cabeca de pancreas: relato de caso

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Oswaldo Luiz; Camargo, Jose Gonzaga [Pontificia Univ. Catolica de Campinas, SP (Brazil). Dept. de Cirurgia; Tornin, Olger de Souza; Botelho, Renato Assayag; Menezes, Marcelo Carneiro [Complexo Hospitalar Heliopolis, Sao Paulo, SP (Brazil). Residencia Medica em Radiologia e Diagnostico por Imagem]. E-mail: olger@ibest.com.br; Chagas, Jose Francisco Sales [Complexo Hospitalar Heliopolis, Sao Paulo, SP (Brazil). Curso de Pos-graduacao em Ciencias da Saude; Souza, Ricardo Pires de [Complexo Hospitalar Heliopolis, Sao Paulo, SP (Brazil). Curso de Pos-graduacao em Ciencias da Saude

    2005-12-01

    The aim of this study is to report a rare case of hepatic artery aneurysm simulating a lesion in the head of the pancreas. A precise evaluation using computed tomography and magnetic resonance imaging is important in the treatment planning, which will influence the prognosis. (author)

  5. Humoral immune responses during experimental infection with Fascioloides magna and Fasciola hepatica in goats and comparison of their excretory/secretory products

    Czech Academy of Sciences Publication Activity Database

    Novobilský, A.; Kašný, M.; Mikeš, L.; Kovařčík, K.; Koudela, Břetislav

    2007-01-01

    Roč. 101, č. 2 (2007), s. 357-364 ISSN 0932-0113 R&D Projects: GA ČR GD524/03/H133; GA MŠk(CZ) LC06009 Grant - others:GA MŠk(CZ) FRVŠ 805/G3/2005 Institutional research plan: CEZ:AV0Z60220518 Keywords : giant liver fluke * sheep liver fluke * humoral immune responses in goats Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.512, year: 2007

  6. Prevalence and intensity of infections in the lymnaeid snail Omphiscola glabra experimentally infected with Fasciola hepatica, Fascioloides magna and Paramphistomum daubneyi

    Czech Academy of Sciences Publication Activity Database

    Dreyfuss, G.; Novobilský, A.; Vignoles, P.; Bellet, V.; Koudela, Břetislav; Rondelaud, D.

    2007-01-01

    Roč. 81, č. 1 (2007), s. 7-12 ISSN 0022-149X R&D Projects: GA ČR GD524/03/H133 Institutional research plan: CEZ:AV0Z60220518 Keywords : digenean infections of lymnaeid snail * intermediate host * free rediae and cercariae Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.155, year: 2007

  7. A comparative study of karyotypes and chromosomal location of rDNA genes in important liver flukes Fasciola hepatica and Fascioloides magna (Trematoda: Fasciolidae)

    Czech Academy of Sciences Publication Activity Database

    Reblánová, M.; Špakulová, M.; Orosová, Martina; Kraľová-Hromadová, I.; Bazsalovicsová, E.; Rajský, D.

    2011-01-01

    Roč. 109, č. 4 (2011), s. 2021-1028 ISSN 0932-0113 Institutional research plan: CEZ:AV0Z60220518 Keywords : SCHISTOSOMA-MANSONI * DIFFERENTIATION * FISH * ITS2 * HYBRIDIZATION * EPIDEMIOLOGY * LOCALIZATION * CYTOGENETICS * SHEEP Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.149, year: 2011

  8. Surgery with radioguided location of a liver metastasis of melanoma choroid: case report; Cirurgia com localizacao radioguiada de uma metastase hepatica de melanoma de coroide: relato de caso

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, Marcelo; Miranda, Mario Henrique Furlanetto, E-mail: mmoreno@unochapeco.edu.br, E-mail: mirandamario@unochapeco.edu.br [Universidade Comunitaria da Regiao de Chapeco (UNOCHAPECO), SC (Brazil)

    2015-04-15

    Introduction: The use of radioguided occult lesion localization prior to surgical excision is increasing, mainly due to the development of new probes and the use of PET-CT. Case report: A 70-year-old male who presented with a metastatic lesion in his liver from a choroidal melanoma. This was located using PET-CT and subsequently located with a low-energy intraoperative gamma probe during the laparotomy. Conclusion: The present case shows that it is possible to excise a hepatic metastasis utilizing the principles of radioguided surgery, even in centers without access to high energy probes. (author)

  9. Non-Hodgkin's lymphoma presenting as a single liver mass; Linfoma nao-Hodgkin apresentando-se como massa hepatica unica

    Energy Technology Data Exchange (ETDEWEB)

    Peixoto, Mila Correia Gois; Peixoto Filho, Anibal Araujo Alves; D' Ippolito, Giuseppe [Hospital Sao Luiz, Sao Paulo, SP (Brazil). Setor de US/TC/RM]. E-mail: scoposl@uol.com.br; Ribeiro, Alessandra Caivano Rodrigues [Hospital Sao Luiz, Sao Paulo, SP (Brazil). Setor de Diagnostico por Imagem

    2009-01-15

    Objective: to describe the main imaging findings of non-Hodgkin's lymphoma presenting as a single liver mass. Materials and methods: a retrospective study was developed with analysis of cases where a single liver mass was observed at ultrasonography, computed tomography and magnetic resonance imaging, and histologically diagnosed as non-Hodgkin's lymphoma. The studies were reviewed by two observers in consensus. Results: three male patients in the fifth decade of life, with non-specific clinical manifestations and single liver mass diagnosed as non-Hodgkin's lymphoma were identified. A hepatic lesion with target sign was observed at ultrasonography in all of the cases. At computed tomography, all the patients presented a heterogeneous, hypodense mass with a ring enhancement. At magnetic resonance imaging, the lesions were heterogeneous and hypointense on T1-weighted and hyperintense on T2-weighted images. Additionally, a ring enhancement was observed in all of the cases after contrast injection. At the moment of the diagnosis, none of the patients presented lymphadenomegaly or involvement of other solid viscera. Conclusion: the diagnosis of hepatic lymphoma should be considered in the presence of a ring-enhanced single liver mass. (author)

  10. Diagnosis of hepatic steatosis by contrast-enhanced abdominal computed tomography; Diagnostico da esteatose hepatica pela tomografia computadorizada de abdome com meio de contraste intravenoso

    Energy Technology Data Exchange (ETDEWEB)

    Monjardim, Rodrigo da Fonseca; Costa, Danilo Manuel Cerqueira; Romano, Ricardo Francisco Tavares; Salvadori, Priscila Silveira; Santos, Jaime de Vargas Conde dos; Atzingen, Augusto Castelli Von; Shigueoka, David Carlos; D' Ippolito, Giuseppe, E-mail: giuseppe_dr@uol.com.br [Universidade Federal de Sao Paulo (EPM/UNIFESP), SP (Brazil). Escola Paulista de Medicina. Dept. de Diagnostico por Imagem

    2013-05-15

    Objective: to evaluate the diagnostic capacity of abdominal computed tomography in the assessment of hepatic steatosis using the portal phase with a simplified calculation method as compared with the non-contrast-enhanced phase. Materials and methods: in the present study, 150 patients were retrospectively evaluated by means of non-contrast-enhanced and contrast-enhanced computed tomography. One hundred patients had hepatic steatosis and 50 were control subjects. For the diagnosis of hepatic steatosis in the portal phase, the authors considered a result of < 104 HU calculated by the formula [L - 0.3 Multiplication-Sign (0.75 Multiplication-Sign P + 0.25 Multiplication-Sign A)] / 0.7, where L, P and A represent the attenuation of the liver, of the main portal vein and abdominal aorta, respectively. Sensitivity, specificity, positive and negative predictive values were calculated, using non-contrast-enhanced computed tomography as the reference standard. Results: the simplified calculation method with portal phase for the diagnosis of hepatic steatosis showed 100% sensitivity, 36% specificity, negative predictive value of 100% and positive predictive value of 75.8%. The rate of false positive results was 64%. False negative results were not observed. Conclusion: The portal phase presents an excellent sensitivity in the diagnosis of hepatic steatosis, as compared with the non-contrast-enhanced phase of abdominal computed tomography. However, the method has low specificity. (author)

  11. Spontaneous and traumatic hepatic rupture: imaging findings and minimally invasive treatment; Ruptura hepatica espontanea e traumatica: aspectos tomograficos e do tratamento minimamente invasivo

    Energy Technology Data Exchange (ETDEWEB)

    Palacio, Glaucia Andrade e Silva; D' Ippolito, Giuseppe [Hospital Sao Luis, Sao Paulo, SP (Brazil). Setor de Disgnostico por Imagem]. E-mail: gl.palacio@bol.com.br; Farias, Andre P. [Hospital Sao Luis, Sao Paulo, SP (Brazil). Setor de Tomografia Computadorizada e Ressonancia Magnetica; Carnevale, Francisco Cesar [Hospital Sao Luis, Sao Paulo, SP (Brazil). Setor de Radiologia Intervencionista; Salem, Marcelo Zindel [Hospital Sao Luis, Sao Paulo, SP (Brazil). Setor de Gastroenterologia Cirurgica; Ricca, Artur Berti [Hospital Sao Luis, Sao Paulo, SP (Brazil)

    2003-12-01

    Spontaneous hepatic bleeding is a rare condition. Our aim was to describe the imaging findings and minimally invasive treatment using transcatheter arterial embolization in patients with spontaneous and traumatic hepatic rupture. Three patients presented acute hemoperitoneum dur to hepatic rupture caused by spontaneous rupture of hepatocellular carcinoma, HELLP syndrome and a blunt hepatic trauma. The patients were submitted to ultrasound and computed tomography of the abdomen and subsequently treated by transcatheter arterial embolization. All patients underwent helical computed tomography before and after treatments. Computed tomography played an important role in the evaluation and follow-up in the therapeutic intervention. Different types of liver injuries were identified. Transcatheter arterial embolization blocked arterial hemorrhage in the patients who were hemodynamically unstable. The conclusion was: transcatheter arterial embolization is an effective and well-tolerated treatment method for the management of hepatic rupture and computed tomography is an excellent method for the diagnosis and follow-up of these patients. (author)

  12. Studies on magnetocaloric and magnetic coupling effects =

    Science.gov (United States)

    Amaral, Joao Cunha de Sequeira

    O presente trabalho apresenta novas metodologias desenvolvidas para a analise das propriedades magneticas e magnetocaloricas de materiais, sustentadas em consideracoes teoricas a partir de modelos, nomeadamente a teoria de transicoes de fase de Landau, o modelo de campo medio molecular e a teoria de fenomeno critico. Sao propostos novos metodos de escala, permitindo a interpretacao de dados de magnetizacao de materiais numa perspectiva de campo medio molecular ou teoria de fenomeno critico. E apresentado um metodo de estimar a magnetizacao espontanea de um material ferromagnetico a partir de relacoes entropia/magnetizacao estabelecidas pelo modelo de campo medio molecular. A termodinamica das transicoes de fase magneticas de primeira ordem e estudada usando a teoria de Landau e de campo medio molecular (modelo de Bean-Rodbell), avaliando os efeitos de fenomenos fora de equilibrio e de condicoes de mistura de fase em estimativas do efeito magnetocalorico a partir de medidas magneticas. Efeitos de desordem, interpretados como uma distribuicao na interaccao magnetica entre ioes, estabelecem os efeitos de distribuicoes quimicas/estruturais nas propriedades magneticas e magnetocaloricas de materiais com transicoes de fase de segunda e de primeira ordem. O uso das metodologias apresentadas na interpretacao das propriedades magneticas de variados materiais ferromagneticos permitiu obter: 1) uma analise quantitativa da variacao de spin por iao Gadolinio devido a transicao estrutural do composto Gd5Si2Ge2, 2) a descricao da configuracao de cluster magnetico de ioes Mn na fase ferromagnetica em manganites da familia La-Sr e La-Ca, 3) a determinacao dos expoentes criticos β e δ do Niquel por metodos de escala, 4) a descricao do efeito da pressao nas propriedades magneticas e magnetocaloricas do composto LaFe11.5Si1.5 atraves do modelo de Bean-Rodbell, 5) uma estimativa da desordem em manganites ferromagneticas com transicoes de segunda e primeira ordem, 6) uma descricao de

  13. Gamma-ray spectrometry, electrical resistivity, and magnetic susceptibility of agricultural soils in the Northwest region of the Parana State, Brazil; Gamaespectrometria, resistividade eletrica e susceptibilidade magnetica de solos agricolas no noroeste do estado do Parana

    Energy Technology Data Exchange (ETDEWEB)

    Becegato, Valter Antonio [Universidade do Estado de Santa Catarina-UDESC, Centro de Ciencias Agroveterinarias, Lages, SC (Brazil); Ferreira, Francisco Jose Fonseca, E-mail: becegato@cav.udesc.br, E-mail: francisco.ferreira@ufpr.br [Universidade Federal do Parana (LPGA/UFPR), Curitiba, PR (Brazil). Dept. de Geologia. Lab. de Pesquisas em Geofisica Aplicada

    2005-10-15

    Gamma-ray spectrometry, electrical resistivity, and magnetic susceptibility measurements were taken from agricultural areas near the City of Maringa, in the Northwest region of the Parana state, south Brazil, in order to characterize the spatial distribution of radionuclides (K, eU, and eTh), the apparent resistivity, and the magnetic susceptibility determined for soils. Three different types of soils are present in this agricultural area: Alfisoil, clayey texture Oxisoil, both deriving from Lower Cretaceous basalts of the Serra Geral Formation; and medium texture Oxisoil from reworked Serra Geral and Goio-Ere formations, the latter deriving from sandstones of the Upper Cretaceous Caiua Group. It could be observed that in more clayey soils both concentration of radionuclides and susceptibility values are higher than in more sandy soils, especially due to the higher adsorption in the former and to the higher availability of magnetic minerals in the latter. The average ppm and Bq Kg{sup -1} grades for K, eU, and eTh in the areas under anthropic activity are of 1766-54.75, 0.83-10.22, and 1.78-7.27, respectively. These grades are significantly higher than those of non-occupied or non-fertilized areas (1101-34.15 K, 0.14-1.69 eU, and 1.31-5.36 eTh in ppm and Bq Kg-1, respectively.) Correlations were observed between uranium and clay, uranium and magnetic susceptibility, uranium and organic matter, and between electric resistivity and clay grades. Varied concentrations of radionuclides were also observed in different fertilizer formulations applied to soy and wheat cultures. Apparent electric resistivity values between 25 and 647 Ohm.m and magnetic susceptibility values between 0.28 e 1.10 x 10-3 SI due to clay and magnetic minerals represented important soil discrimination factors in the study area that can be incorporated as easy, low-cost soil mapping tools. (author)

  14. Structural and magnetic Ni-Zn ferrite synthesized by combustion reaction and sintered in a conventional oven; Caracterizacao estrutural e magnetica de ferrita Ni-Zn sintetizadas por reacao de combustao e sinterizadas em forno convencional

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, D.A.; Diniz, V.C.S.; Costa, A.C.F.M., E-mail: deboralbq@hotmail.com [Universidade Federal de Campina Grande (UFCG), Campina Grande, PB (Brazil). Departamento de Engenharia de Materiais; Kiminami, R.H.G.A. [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil). Departamento de Engenharia de Materiais; Cornejo, D.R. [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Instituto de Fisica. Departamento de Fisica dos Materiais e Mecanica

    2011-07-01

    The Ni-Zn ferrite due to their electrical and magnetic properties allows use in various technological applications. These properties can be controlled through appropriate choice of chemical composition, structural characteristics and morphology of the powders used and the techniques used for sintering. Thus, this study aims to evaluate the sintering in a conventional oven at a temperature of 1200 deg C/2h samples of Ni-Zn ferrite synthesized by microwave energy. The samples were characterized by density measurement, XRD, SEM and magnetic measurements. The results indicate the phase formation of Ni-Zn ferrite crystalline phase with crystallite size of 80 nm. The sample was heterogeneous microstructure with grain size of about 1 μm high intergranular porosity. The sample showed the saturation magnetization of 7.57 emu/g, coercive field and remanent magnetization close to zero, thus indicating a behavior characteristic of superparamagnetic materials. (author)

  15. Clinical recommendations of cardiac magnetic resonance, Part I: ischemic and valvular heart disease: a position paper of the working group 'Applicazioni della Risonanza Magnetica' of the Italian Society of Cardiology.

    Science.gov (United States)

    Aquaro, Giovanni Donato; Di Bella, Gianluca; Castelletti, Silvia; Maestrini, Viviana; Festa, Pierluigi; Ait-Ali, Lamia; Masci, Pier Giorgio; Monti, Lorenzo; di Giovine, Gabriella; De Lazzari, Manuel; Cipriani, Alberto; Guaricci, Andrea I; Dellegrottaglie, Santo; Pepe, Alessia; Marra, Martina Perazzolo; Pontone, Gianluca

    2017-04-01

    Cardiac magnetic resonance (CMR) has emerged as a reliable and accurate diagnostic tool for the evaluation of patients with cardiac disease in several clinical settings and with proven additional diagnostic and prognostic value compared with other imaging modalities. This document has been developed by the working group on the 'application of CMR' of the Italian Society of Cardiology to provide a perspective on the current state of technical advances and clinical applications of CMR and to inform cardiologists on how to implement their clinical and diagnostic pathways with the inclusion of this technique in clinical practice. The writing committee consisted of members of the working group of the Italian Society of Cardiology and two external peer reviewers with acknowledged experience in the field of CMR.

  16. Numerical Calculation of Transport Based on the Drift-Kinetic Equation for Plasmas in General Toroidal Magnetic Geometry: Numerical Methods; Calculo Numerico de Transporte mediante la Ecuacion Cinetica de Deriva para Plasmas en Geometria Magnetica Toroidal: Metodos Numericos

    Energy Technology Data Exchange (ETDEWEB)

    Reynolds, J. M.; Lopez-Bruna, D.

    2009-10-12

    In this report we continue with the description of a newly developed numerical method to solve the drift kinetic equation for ions and electrons in toroidal plasmas. Several numerical aspects, already outlined in a previous report [Informes Tecnicos Ciemat 1165, mayo 2009], will be treated now in more detail. Aside from discussing the method in the context of other existing codes, various aspects will be now explained from the viewpoint of numerical methods: the way to solve convection equations, the adopted boundary conditions, the real-space meshing procedures along with a new software developed to build them, and some additional questions related with the parallelization and the numerical integration. (Author) 16 refs.

  17. Annealing Effects on the Magnetization of Co-Ni-B Amorphous Nanoparticles; Propiedades Magneticas de Nanoparticulas Monodominio de la Familia (Co{sub x}Ni {sub 1-x}){sub 70}B{sub 30}

    Energy Technology Data Exchange (ETDEWEB)

    Vargas, J M [Instituto Balseiro, Universidad Nacional de Cuyo, Centro Atomico Bariloche (Argentina)

    2001-07-01

    Chemically prepared (Co{sub x}Ni {sub 1-x}){sub 1}00{sub -y}B{sub y} (x=0.5, 0.75, 1; y{approx}30) amorphous fine particles were characterized by x-ray diffraction, DTA and TGA, and in-situ magnetic measurement as a function of annealing temperature in an inert atmosphere.Magnetic measurement performed in as prepared and {approx}150C degree annealed samples show an increase of the saturation magnetization and magnetic moment after thermal tretment.Room temperature magnetization increases by factors of {approx}3 in average.These measurements may indicate a local re-ordering of the amorphous phase at temperatures much lower than the full crystallization temperature.

  18. Equivalent Coil Model for Computing Levitation Forces Between Permanent Magnets and High Temperatures Superconductors; Modelo de Espira Equivalente para el Calculo de Fuerzas de Levitacion Magnetica entre Imanes Permanentes y Superconductores de Alta Temperatura Critica

    Energy Technology Data Exchange (ETDEWEB)

    Cavia Santos, S; Garcia-Tabares, L

    1998-05-01

    A new simple theory has been developed for the study of levitation forces between a permanent magnet and a HTc superconductor. This theory is based on the assumption that both, the magnet and the superconductor, can be modelled by an equivalent coil placed on their surface. While the current flowing through the permanent magnet is constant, the equivalent current through the superconductor can be iether corresponding to screen the overall flux or a constant current corresponding to critical current density when the superconductor is saturated. A test facility has been designed and built for measuring levitation forces at variable approaching speeds. Comparison between theoretical and experimental measurements are presented in the report as well as a general description of the test facility. (Author)

  19. Study of the influence of zirconium and gallium on the magnetic properties and microstructures of praseodymium-based permanent magnets; Estudo da influencia do zirconio e galio nas propriedades magneticas e na microestrutura dos imas permanentes a base de praseodimio

    Energy Technology Data Exchange (ETDEWEB)

    Fusco, Alexandre Giardini

    2006-07-01

    In this work was studied the influence of the addition of 0.5 at. % of zirconium and gallium on praseodymium-based HD sintered magnets obtained using a mixture of alloys. The alloys used in this study were: Pr{sub 12.6}Fe{sub 68.3}Co{sub 11.6}B{sub 6}Zr{sub 0.5}Ga{sub 1}, Pr{sub 16}Fe{sub 75.5}B{sub 8}Zr{sub 0.5}, Pr{sub 13}Fe{sub 80.5}B{sub 6}Zr{sub 0.5}. The investigation started by measuring the magnetic properties and observing the microstructure of the magnets. After that, the magnets were annealed at 1000 deg C for 2 hours followed by rapid cooling, in a total of 10 hours. This heat treatment was followed by 5 hours at the same temperature up to a total of 35 hours. Changes in the microstructure were compared to the change in the magnetic properties aiming at a proper understanding of the role of each added element in relation to the magnetically hard phase (phase {phi}). It has been shown that gallium and zirconium act as grain refiners of the matrix phase {phi}. Gallium acts in the grain and favoring of the shape stability and improvement of the magnetic properties. For the Pr{sub 14.3}Fe{sub 71.9}Co{sub 5.8}B{sub 7}Zr{sub 0.5}Ga{sub 0.5} sintered magnet the evolution of the magnetic properties after 15 hours heat treatment was: remanence from (1.25{+-}0.02) T to (1.30{+-}0.02) T, intrinsic coercivity from (1.11{+-}0.02) T to (0.87{+-}0.02) T, squareness factor from (0.68{+-}0.02) to (0.82{+-}0.02) and energy product from (285{+-}5) kJ/m{sup 3} to (317{+-}5) kJ/m{sup 3}. Zirconium has two effects on the sintered magnets. Firstly, avoiding random grain growth and enhancing anisotropy. However, by concentrating on the grain boundaries, yield reverse domains and is detrimental to the intrinsic coercivity. For the sintered Pr{sub 14.5}Fe{sub 78}B{sub 7}Zr{sub 0.5} magnet the evolution of the magnetic properties achieved after a heat treatment of 15 hours was: remanence from (1.19{+-}0.02) T to (1.25{+-}0.02) T, coercivity from (0.74{+-}0.02) T to (0.94{+-}0.02) T, squareness factor from (0.88{+-}0.02) to (0,85{+-}0.02) and energy product from (258{+-}5) kJ/m{sup 3} to (291{+-}5) kJ/m{sup 3}. For the Pr{sub 16}Fe{sub 75.5}B{sub 8}Zr{sub 0.5} sintered magnet the evolution of magnetic properties after 20 hours heat treatment was: remanence from (1.17{+-}0,02) T to (1.24{+-}0.02) T, coercivity from (0.90{+-}0,02) T to (1.22{+-}0.02) T, squareness factor from (0,93{+-}0,02) to (0.66{+-}0.02) and energy product from (258{+-}5) kJ/m{sup 3} to (291{+-}5) kJ/m{sup 3}. (author)

  20. Magnetic and microstructural properties of Ni-Zn ferrites synthesized and sintered by microwave energy; Propriedades magneticas e microestruturais de ferritas Ni-Zn sintetizadas e sinterizadas oir energia de microondas

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, D.A.; Diniz, V.C.S.; Sousa, J-P.LM.L.; Costa, A.C.F.M. [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Dept. de Engenharia de Materiais; Kiminami, R.H.G.A. [Universidade Federal de Sao Carlos (UFScar), SP (Brazil). Dept. de Engenharia de Materiais; Cornejo, D.R. [Universidade de Sao Paulo (IF/USP), SP (Brazil). Inst. de Fisica

    2009-07-01

    The soft ferrites (or soft) and the Ni-Zn type are composed of spinel with cubic structure, which exhibit a permanent magnetization, called ferrimagnetism. Thus, this work will be assessed the structure and magnetic properties of ferrites Ni{sub 0},{sub 5}Zn{sub 0},{sub 5}Fe{sub 2}O{sub 4} prepared by combustion reaction using microwave energy as a source of heat and urea as fuel and after sintering by microwave energy. The synthesized powders were compacted by uniaxial pressing. The synthesized powders and the samples after sintering were characterized by XRD, SEM and magnetic measures. The diffractogram X-ray powder and the sintered samples showed the presence of the desired phase Ni{sub 0},{sub 5}Zn{sub 0},{sub 5}Fe{sub 2}O{sub 4} in both cases. The powders and sintered samples resulted in the Ms value of 8.09 emu/g and 67.73 emu/g, respectively. (author)

  1. Magnetic configuration effects on plasma transport under Neutral Beam Injection at TJ-II (Simulation); Efectos de Configuracion Magnetica en el Transporte de Plasma durante la Inyeccion de Haces Neutros en el TJ-II (Simulacion)

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J.; Fuentes, C.; Liniers, M.

    2001-07-01

    A systematic analysis of magnetic configurations (27 in total), using a Transport model including impurity dynamics and sputtering effects has been done. For small size configurations or those close to rational t values there is radioactive collapse, independently of the external gas puffing (GP) strategy chosen. The reason is the insufficiency of observed power, either by the high shine through losses due to their low radii, or by the increase of fast ion orbit losses near the resonances. For the majority of configurations without collapse, fast ion orbit losses for CO injection (going in the same direction than the toroidal magnetic field) are higher, and in consequence the power absorption and the plasma {beta} achieved are laser, than for the opposite direction. Nevertheless in the region placed just above the main resonances (1/3 and 1/2 per period) this situation reverses. The reasons have been analysed and explained at previous studies. A consequence of this fact is that the optima of confinement for the Counter case are shifted towards higher t values than the CO one, with higher plasma {beta}, except near the resonances. As usual the balanced case is in between. The optima achieving stationary state are very close (and often are coincident) with those lacking that restriction. The best configuration (highest average {beta}) for balanced injection, with <{beta}>=1.1% and central value 3.2%, although in this region the results are rather insensitive to configuration and GP strategy. The configurations placed around the 100{sub 4}4 would need also the lowest power entering the torus in order to avoid collapse and to achieve an acceptable NBI absorption level. (Author) 12 refs.

  2. Influence of high energy phosphate metabolism in postischemic myocardial dysfunction using magnetic resonance spectroscopy; Influencia dos fosfatos de alta energia na funcao ventricular em pacientes com infarto do miocardio avaliada pela resonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Kalil Filho, Roberto [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Hospital das Clinicas

    1998-05-01

    The recovery of left ventricular function after reperfusion is delayed in general by several hours, days or weeks and this phenomenon is known as myocardial stunning. One of the theories to explain the pathogenesis of this postischemic myocardial dysfunction is the production of not enough energy by mitochondria, leading to decreased adenosine-triphosphate (ATP) levels. We evaluated the influence of high energy phosphate metabolism in postischemic myocardial dysfunction, using magnetic resonance spectroscopy in patients with acute anterior wall myocardial infarction, successfully reperfused, within the first six hours from the onset of the symptoms. Twenty-nine patients were studied in the acute phase (on average four days after the onset of myocardial infarction) and 21 repeated the examination in the follow-up phase (average 39 days). Regional left ventricular function was evaluated by cine-resonance and high energy phosphate metabolism by phosphorus-31 spectroscopy, using the phosphocreatine {beta} ATP (P Cr/{beta}ATP) ratio. The existence of myocardial stunning was suggested by the improvement of the related regional contractility during the follow-up. The contractility improved in the septal wall from 2.46{+-} 0.68 to 1.54 {+-} 0.78 (p<0.001), in the anteroseptal wall from 2.0 {+-} 0.89 to 1.40 {+-} 0.75 (p<0.001) and in the anterior wall from 2.37 {+-} 0.71 to 1.41 {+-} 0.59 (p<0.001). The P Cr/{beta}ATP ratio did not change from acute to follow-up phase (1.51 {+-} 0.17 vs. 1.53 {+-} 0.17; p = 0.6). This study suggests that decreased high energy phosphate metabolism after reperfusion does not have an important role in the genesis of the myocardial stunning in patients with acute anterior wall myocardial infarction. (author) 25 refs., 9 figs., 1 tab.

  3. A prospective, comparative study of magnetic resonance cholangipancreatography and direct cholangiography in the diagnosis of biliary diseases; Estudio propsective comparativo en el diagnostico de la patologia biliar. Colangiopancreatografia por resonancia magnetica frente a colangiografia directa

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, E.; Falco, J.; Martin, J.; Brullet, E.; Campo, R.; Espinos, J.; Darnell, A.

    2001-07-01

    To assess the sensitivity and specificity of magnetic resonance cholangiopancreatography (MRCP) as a noninvasive diagnostic technique, comparing it with endoscopic retrograde cholangiopancreatography (ERCP) and percutaneous transhepatic cholangiography (PTHC) in the diagnosis of biliary disease, focusing particularly on patients with choledocholithiasis. Between June 1997 and february 1998, 109 patients referred by the Corporacio Sanitaria del Parc Tauli and the Hospital Mutua de terrasa in Barcelona, Spain were evaluated. MRCP and ERCP or PTHC were performed in every case, always in that order and separated by a maximum of 7 days. Twenty-four patients were excluded from the study for different reasons, leaving a series of 85 patients. All the studies were performed with a 1-Tesla Siemens Magneton Impact Expert using half-fourier single-short turbo spin-echo (HASTE) and rapid acquisition with relaxation enhancement (RARE) methods in several coronal and axial planes. The MRCP readings were carried out by consensus by two radiologists who are experts in the assessment of diseases of the digestive tract. In this series of 85 patients; MCRP showed a sensitivity of 98.4%, a specificity of 94.7%, a positive predictive value of 98.4% and a negative predictive value of 94.7% for the detection of biliary diseases. For the detection choledocholithiasis, these values were 100%, 89.5%, 88% and 100%, respectively. MRCP is extremely reliable in the diagnosis of biliary diseases, especially in cases of choledocholithiasis. Its high negative predictive value obviates the need for other invasive diagnostic tests. (Author) 30 refs.

  4. Complexity Index as Applied to Magnetic Resonance: Study Based on a Scale of Relative Units; Indice de complejidad en resonancia magnetica: estudio basado en una escala de unidades relativas

    Energy Technology Data Exchange (ETDEWEB)

    Capelastegui, A.; Villanua, J.

    2003-07-01

    To analyze the merit and repercussions of measuring magnetic resonance (MR) activity in units of radiological activity, and of using complexity index (CI) as an activity indicator. We studied the MR activity of Osatek, Inc. during an 8-year period (1994-2001). We measured this activity both in number of MR procedures performed and in units of radiological activity, such units being based on the scale of relative units published in the Radiological Services Administration Guidelines published by the Spanish Society or Medical Radiology. We calculated the annual complexity index, this being a quotient between the number of MR procedures performed and corresponding value in units of radiological activity. We also analyzed factors that can have an impact on the CI: type of exploration and power of the equipment's magnetic field. The CL stayed practically stable during the first 4 years of the study, while it increased during the second 4 years. There exists a direct relationship between this increase and the percentage of explorations that we term complex (basically, body-and angio-MR). The increasing complexity of MR studies in the last years is evident from a consideration of CI. MR productivity is more realistically expressed in units of radiological activity than in number of procedures performed by any one center. It also allows for making external comparisons. CI is a useful indicator that can be utilized as an administrative tool. (Author) 13 refs.

  5. Intelligent simulated annealing algorithm applied to the optimization of the main magnet for magnetic resonance imaging machine; Algoritmo simulated annealing inteligente aplicado a la optimizacion del iman principal de una maquina de resonancia magnetica de imagenes

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez Lopez, Hector [Universidad de Oriente, Santiago de Cuba (Cuba). Centro de Biofisica Medica]. E-mail: hsanchez@cbm.uo.edu.cu

    2001-08-01

    This work describes an alternative algorithm of Simulated Annealing applied to the design of the main magnet for a Magnetic Resonance Imaging machine. The algorithm uses a probabilistic radial base neuronal network to classify the possible solutions, before the objective function evaluation. This procedure allows reducing up to 50% the number of iterations required by simulated annealing to achieve the global maximum, when compared with the SA algorithm. The algorithm was applied to design a 0.1050 Tesla four coil resistive magnet, which produces a magnetic field 2.13 times more uniform than the solution given by SA. (author)

  6. Design of a mannequin for spectroscopy magnetic resonance of proton. Preliminary results of its application; Diseno de un maniqui para espectroscopia por resonancia magnetica de proton. resultados preliminares de su aplicacion

    Energy Technology Data Exchange (ETDEWEB)

    Parra Osoirio, V.; Galan Montenegro, P.; Moreno Saiz, C.; Garcia-Alvarez, R.; Arrabal Nunez, S.; Caudepon Moreno, F.; Navarro Guirado, F.; Asensjo Garcia, B.

    2013-07-01

    The proton magnetic resonance spectroscopy is a technique of metabolic Imaging allowing in vivo measurement of the levels of different metabolites. Is under increasing development and has shown an improvement of diagnostic indexes and therapeutic, since it allows: differential diagnosis between injuries, determination of tumor grade of Pre surgical form, determination of the presence of a lesion until it is clear, definition of progress and evaluation of the effects of a treatment. The aim in this work is to have a mannequin that allows analyze and adjust various parameters involved in the spectroscopic process, and establish a quantification of these parameters. (Author)

  7. Qualitative characterization of free polyamines in ethyleneamines epoxide hardeners by nuclear magnetic resonance spectroscopy; Caracterizacion cualitativa de poliaminas libres en endurecedores de resinas epoxidicas del tipo etilenaminas por espectroscopia de resonancia magnetica nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Garcia, Filiberto [Universidade Federal de Itajuba (UNIFEI), MG (Brazil); Miguez, Eduardo; Tavares, Maria Ines B. [Universidade Federal do Rio de Janeiro (UFRJ/IMA), RJ (Brazil). Inst. de Macromoleculas Professora Eloisa Mano

    2008-01-15

    The qualitative characterization of two commercial ethyneamines epoxide hardeners marketed by ACROS (U.S.A.) was carried out by using carbon-13 nuclear magnetic resonance spectroscopy. The products were triethylenetetramine (TETA) and tetraethylenepentamine (TEPA). TETA had four components, the most important one being triethylenetetramine, an ethyleneamine of lineal structure, in a concentration of 60 mol%. Another component had ramified structure, while the other two exhibited recurrent structures of the piperazina type. TEPA had five components with similar structures. The major component was tetraethylenepentamine in an approximate concentration of 55 mol%. These results agree with the composition of similar products marketed by Dow Chemical Company, namely DEH 24 and DEH 26, respectively. (author)

  8. Noninvasive study of anatomic variations of the bile and pancreatic duct using magnetic resonance cholangiopancreatography; Estudio no invasivo de variantes anatomicas de la via biliar y pancreatica mediante colangiopancreatografia por resonancia magnetica (CPRM)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, E.; Falco, J.; Campo, R.; Martin, J.; Brullet, E. [SDI-UDIAT Corporacio Sanitaria Parc Tauli. Sabadell (Spain); Espinos, J. [Hospital Mutua de Tarrasa (Spain)

    1999-07-01

    To identify anatomic variations of the bile duct and pancreatic duct and papillary anomalies by means of magnetic resonance cholangiopancreatography (MRCP) and determine their correlation with endoscopic retrograde cholangiopancreatography (ERCP) findings. Eighty-five patients were selected by means of a prospective study comparing MRCP and ERCP. Coronal and axial HASTE images and coronal and oblique coronal RARE images were acquired in all the patients. Four of the studies (6%) were excluded because of poor technical quality. Anatomic variations were observed in 26 cases (30.5%), including trifurcation (n=7; 27%), right hepatic duct draining into left hepatic duct (n=2, 7.7%), right hepatic duct draining into common bile duct (n=4; 15.4%), extrahepatic confluence (n=2; 7.7%), medial cystic duct (n=2; 7.7%), parallel cystic duct (n=3; 11.5%), juxtapapillary duodenal diverticulum (n=3; 11.5%) and pancreas divisum (n=3; 11.5%). A good correlation was observed between the MRCP and ERCP findings. The introduction of MRCP into the noninvasive study of biliary disease may be useful in the detection of anatomic variations relevant to laparoscopic surgery and other endoscopic and interventional techniques. (Author) 11 refs.

  9. Magnetic spires for the detection of the position of the plasma column in a Tokamak (linear approximation); Espiras magneticas para la deteccion de la posicion de la columna de plasma en un Tokamak (aproximacion lineal)

    Energy Technology Data Exchange (ETDEWEB)

    Colunga S, S

    1990-07-15

    In this report the simplified analysis of a method to detect the movement of the plasma column of a tokamak in the vertical direction and of the biggest radius is given. The peculiar case of the Tokamak Novillo of the Plasma Physics Laboratory of the ININ is studied. (Author)

  10. Clinical recommendations of cardiac magnetic resonance, Part II: inflammatory and congenital heart disease, cardiomyopathies and cardiac tumors: a position paper of the working group 'Applicazioni della Risonanza Magnetica' of the Italian Society of Cardiology.

    Science.gov (United States)

    Pontone, Gianluca; Di Bella, Gianluca; Silvia, Castelletti; Maestrini, Viviana; Festa, Pierluigi; Ait-Ali, Lamia; Masci, Pier Giorgio; Monti, Lorenzo; di Giovine, Gabriella; De Lazzari, Manuel; Cipriani, Alberto; Guaricci, Andrea I; Dellegrottaglie, Santo; Pepe, Alessia; Marra, Martina Perazzolo; Aquaro, Giovanni D

    2017-04-01

    The current document was developed by the working group on the 'application of cardiac magnetic resonance' of the Italian Society of Cardiology to provide a perspective on the current state of technical advances and clinical cardiac magnetic resonance applications and to inform cardiologists how to implement their clinical and diagnostic pathway with the introduction of this technique in the clinical practice. Appropriateness criteria were defined using a score system: score 1-3 = inappropriate (test is not generally acceptable and is not a reasonable approach for the indication), score 4-6 = uncertain (test may be generally acceptable and may be a reasonable approach for the indication but more research and/or patient information is needed to classify the indication definitively) and score 7-9 = appropriate (test is generally acceptable and is a reasonable approach for the indication).

  11. Numerical Calculation of Transport Based on the Drift Kinetic Equation for plasmas in General Toroidal Magnetic Geometry; Calculo Numerico del Transporte mediante la Ecuacion Cinetica de Deriva para Plasmas en Geometria Magnetica Toroidal: Preliminares

    Energy Technology Data Exchange (ETDEWEB)

    Reynolds, J. M.; Lopez-Bruna, D.

    2009-12-11

    This report is the first of a series dedicated to the numerical calculation of the evolution of fusion plasmas in general toroidal geometry, including TJ-II plasmas. A kinetic treatment has been chosen: the evolution equation of the distribution function of one or several plasma species is solved in guiding center coordinates. The distribution function is written as a Maxwellian one modulated by polynomial series in the kinetic coordinates with no other approximations than those of the guiding center itself and the computation capabilities. The code allows also for the inclusion of the three-dimensional electrostatic potential in a self-consistent manner, but the initial objective has been set to solving only the neoclassical transport. A high order conservative method (Spectral Difference Method) has been chosen in order to discretized the equation for its numerical solution. In this first report, in addition to justifying the work, the evolution equation and its approximations are described, as well as the baseline of the numerical procedures. (Author) 28 refs.

  12. Numerical Calculation of Transport Based on the Drift-Kinetic Equation for Plasmas in General Toroidal Magnetic Geometry: Convergence and Testing; Calculo Numerico del Transporte mediante la Ecuacion Cinetica de Deriva para Plasmas en Geometria Magnetica Toroidal: Convergencia y Comprobaciones

    Energy Technology Data Exchange (ETDEWEB)

    Reynolds, J. M.; Lopez-Bruna, D.

    2009-12-11

    This report is the third of a series [Informes Tecnicos Ciemat 1165 y 1172] devoted to the development of a new numerical code to solve the guiding center equation for electrons and ions in toroidal plasmas. Two calculation meshes corresponding to axisymmetric tokamaks are now prepared and the kinetic equation is expanded so the standard terms of neoclassical theory --fi rst order terms in the Larmor radius expansion-- can be identified, restricting the calculations correspondingly. Using model density and temperature profiles for the plasma, several convergence test are performed depending on the calculation meshes and the expansions of the distribution function; then the results are compared with the theory [Hinton and Hazeltine, Rev. Mod. Phys. (1976)]. (Author) 18 refs.

  13. Synthesis, characterization and study of the magnetic properties of a coordination polymer containing cobalt(II) and copper(II); Sintese, caracterizacao e estudo das propriedades magneticas de um polimero de coordenacao contendo cobalto(II) e cobre(II)

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, Tamyris T. da; Stumpf, Humberto O.; Pereira, Cynthia L.M., E-mail: cynthialopes@ufmg.br [Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Pires, Heber S.; Oliveira, Luiz F.C. de [Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Juiz de Fora, MG (Brazil); Pedroso, Emerson F. [Departamento de Quimica, Centro Federal de Educacao Tecnologica de Minas Gerais, Belo Horizonte, MG (Brazil); Nunes, Wallace C. [Instituto de Fisica, Universidade Federal Fluminense, Niteroi, RJ (Brazil)

    2012-07-01

    This work describes the synthesis and characterization of two new compounds with ligand opy (N-(2-pyridyl)oxamate): the copper(II) precursor [Me{sub 4}N]{sub 2}[Cu(opy){sub 2}].5H{sub 2}O and Co{sup II} Cu{sup II} coordination polymer {l_brace}[Co Cu(opy){sub 2}]{r_brace}{sub n}x4nH{sub 2}O. This latter compound was obtained by reaction of [Me{sub 4}N]{sub 2}[Cu(opy){sub 2}].5H{sub 2}O and CoCl{sub 2}.6H{sub 2}O in water. The heterobimetallic Co{sup II} Cu{sup II} chain was characterized by elemental analysis, IR spectroscopy, thermogravimetry and magnetic measurements. Magnetic characterization revealed typical behavior of one-dimensional (1D) ferrimagnetic chain as shown in the curves of temperature (T) dependence of magnetic susceptibility ({chi}{sub M}), in the form of {chi}{sub M}T versus T, and dependence of magnetization (M) with applied field (H). (author)

  14. Serial contrast-enhanced magnetic resonance and magnetization transfer in the study of patients with multiple sclerosis; Resonancia magnetica con contraste y transferencia de magnetizacion en el estudio seriado de pacientes con esclerosis multiple

    Energy Technology Data Exchange (ETDEWEB)

    Rovira, A; Alonso, J; Cucurella, G; Nos, C; Tintore, M; Pedraza, S; Rio, J; Montalban, X [Hospital General i Universitari Vall d` Hebron. Barcelona (Spain)

    1998-12-31

    To demonstrate the changes in the magnetization transfer ratio (MTR) of different demyelinating plaques, correlating them with the baseline values in T1-weighted contrast-enhanced magnetic resonance (MR) sequences in order to relate them more closely to the underlying disease. The study was based on 33 demyelinating plaques obtained from six patients clinically diagnosed as having remitting-recurring multiple sclerosis (MS). All the patients underwent two MR studies at a 3 to 5-month interval, including contrast-enhanced T1 and T2- weighted sequences and magnetization transfer images. The latter were used to calculate the MTR for each of the demyelinating plaques included in the study. The statistical analysis of the results obtained revealed statistically significant between initial MTR values and those of subsequent T1-weighted sequences. The MTR demonstrate significant differences between plaques according to contrast-enhanced T1-weigh tes sequences, probably indicating variable degrees of edema, demyelination and tissue destruction. These differences should be taken into account to enable the use of T1-weighted sequences to quantify the lesion load in MS patients. (Author) 35 refs.

  15. Magnetic nanoparticles of NiCuZn tested in different conditions in catalysis for biodiesel; Nanoparticulas magneticas de NiCuZn testadas em diferentes condicoes na catalise para biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Dantas, J.; Silva, F.N.; Silva, A.S.; Pereira, K.R.O.; Costa, A.C.F.M., E-mail: joeldadantas@yahoo.com.br [Universidade Federal de Campina Grande (LabSMaC/UFCG), PB (Brazil). Laboratorio de Sintese de Materiais Ceramicos

    2014-07-01

    In this work it was used magnetic nanoparticles Ni{sub 0,2}Cu{sub 0,3}Zn{sub 0,5}Fe{sub 2}O{sub 4}, chemical and thermally stable, under different conditions in catalysis for biodiesel. The magnetic characteristic of such material allows the catalyst recovery after the reactions by applying a permanent magnet. It was proposed to evaluate the performance of the nanomagnetic catalyst Ni{sub 0,2}Cu{sub 0,3}Zn{sub 0,5}Fe{sub 2}O{sub 4} in the transesterification, modifying the processing variables (temperature, time, molar ratio of oil:alcohol and catalyst amount). The nanoparticles were synthesized by combustion reaction and characterized by XRD, TG, BET, magnetic measurements and gas chromatography. The results revealed the formation of inverse spinel phase, type B(AB){sub 2}O{sub 4}, presenting isotherm profile classified as type V, with hysteresis loop of type 3 (H3). The magnetic hysteresis curve showed a characteristic behavior of soft magnetic material. GC analysis confirmed that nanoparticles were catalytically active, since they were superior to the reaction conducted without the catalyst presence. Besides, the reactions suffered considerable influence due to the changes of the independent variables. (author)

  16. Calculation of the electronic and magnetic structures of 3d impurities in the Hcp Fe matrix; Calculo da estrutura eletronica e magnetica de impurezas 3d na matriz do Fe HCP

    Energy Technology Data Exchange (ETDEWEB)

    Franca, Fernando

    1995-12-31

    In this work we investigate the local magnetic properties and the electronic structure of HCP Fe, as well introducing transition metals atoms 3d (Cs, Ti, Cr, Mn, Co, Ni, Cu, Zn) in HCP iron matrix. We employed the discrete variational method (DVM), which is an orbital molecular method which incorporate the Hartree-Fock-Slater theory and the linear combination of atomic orbitals (LCAO), in the self-consistent charge approximation and the local density approximation of Von Barth and Hedin to the exchange-correlation potential. We used the embedded cluster model to investigate the electronic structure and the local magnetic properties for the central atom of a cluster of 27 atoms immersed in the microcrystal representing the HCP Fe. (author) 32 refs., 19 figs., 2 tabs.

  17. Preparation of combustible material from high sulphur coal by means of pyrolysis: magnetic separation; Obtencion de combustibles limpios a partir de carbones con altos contenidos en azure mediante procesos de pirolisis: separacion magnetica

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-11-01

    Basic studies on coal desulphurization by pyrolysis have been carried out with a series of low rank coals with high total sulphur contents and differences in the distribution of sulphur forms. The evolved sulphur compounds were studied by sulphide selective electrode H{sub 2}S and Fourier transform infrared (FTIR) spectroscopy. The mechanisms affecting the sulphur removal during pyrolysis have been studied by scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX), X-ray diffraction (XRD) and photoelectronic spectroscopy (XPS). A sample coal of 11 Tm, representative of the Teruel basins was processed at pilot scale in a rotary kiln (coal HR). A series of pyrolysis runs simulating the experimental conditions of the rotary kiln were also carried out in laboratory scale. The magnetic behaviour of the chars from the rotary kiln and from the lab-scale pyrolysis was tested. The efficiency of the desulphurization, including pyrolysis and magnetic separation, was calculated. Chars from rotary kiln were tested by thermogravimetric analysis, air reactivity and carbon efficiency combustion in fluidized bed.

  18. Biodegradable Bioadherent Microcapsules for Orally Administered Sustained Release Vaccines

    Science.gov (United States)

    1997-05-01

    Fasciola hepatica which is a known bioadhesive’’. The encapsulation method is the classical, well described water in oil technique for the preparation of...immunization, Vaccine 12 (1994) 387-340. 6. Waite, J.H., Rice-Ficht, A.C., Presclerotized eggshell protein from the liver fluke Fasciola hepatica...Biochemistry 26 (1987) 7819-7825. 7. Waite, J.H., Rice-Ficht, A.C., Eggshell precursor proteins of Fasciola hepatica: II. Microheterogeneity in vitelline

  19. Orally Administered Bioadherent Sustained Release Microencapsulated Vaccines

    Science.gov (United States)

    1996-09-01

    microspheres consist of bovine serum albumin (BSA) and recombinant vitelline protein B (vpB) from the liver fluke Fasciola hepatica which is a known...from the liver fluke Fasciola hepatica." Biochemistry 26, 7819-7825. 10. Waite, J.H., Rice-Ficht, A.C. (1992) "Eggshell precursor proteins of Fasciola ...precursor proteins of Fasciola hepatica: I. Structure and expression of vitelline protein B." Mol. Bioch. Parasitol. 54, 127-143. 12. Yapel, A.F. (1985

  20. Study of the association degree between cerebral magnetic resonance signal and the transcranial sonography Estudio del grado de asociación entre la señal de resonancia magnética cerebral y la ecografía transcraneal Estudi del grau d'associació entre el senya de ressonancia magnètica cerebral i l'ecografia transcranial

    OpenAIRE

    Carrascosa Morales, Rafael

    2012-01-01

    El coregistre d'una imatge d'ecografia transcraneal amb un volum de ressonància magnètica cerebral i estudi del grau d'associació entre elles. Projecte el·laborat amb Transmural Biotech SL English: The study tries to achieve a multimodal registration between a transcranial sonography (TCS) and a cerebral magnetic resonance volume. After the registration stage, a statistical analysis of each brain matter (white matter, gray matter and cerebrospinal fluid) was performed to demonstrate the...

  1. Electron paramagnetic resonance of Na, [(FeEDTA){sub 2}oJ-12H{sub 2}0] crystal electrons; Ressonancia paramagnetica de eletrons de cristais de Na, [(FeEDTA){sub 2}oJ-12H{sub 2}0

    Energy Technology Data Exchange (ETDEWEB)

    Esquivel, Darci Motta de Souza

    1974-07-01

    Crystals of Na [(Fe EDTA){sub 2}o] {center_dot}12H{sub 2}0 were investigated by means of electron paramagnetic resonance spectroscopy. The spectra were obtained at various temperatures and crystals orientations. These spectra are very complex with many absorption bands. As the crystal orientation with respect to the magnetic field was changed the variations of the intensity and number of bands were recorded. The antiferromagnetic coupling between the iron atoms in the bridge Fe - 0 - Fe gives rise to states with total spin quantum number S= 0, 1, 2, 3, 4 and 5. Analyses of the EPR spectra as a function of temperature provided a means for the identification of the EPR absorption bands attributed to the states with S = 2. It was also possible to calculate the exchange parameter value J = 300 K. From the study of bands angular dependence in relation to the crystal orientation in the magnetic field it was found that the magnetic crystal axes X, Y, Z and the crystals axes a, b, c are related as (a, b, c) = (Y, Z, X) {exclamation_point} with a precision of 5 deg. Also the crystalline distortion parameters were calculated D = 0.21 {+-} 0.02 cm{sup 1}; E = 0.015 {+-} 0.005 cm{sup 1}. (author)

  2. Procedures of hepatic scintigraphy and improvement of professionals by using anthropomorphic simulator object of liver in nuclear medicine; Procedimentos de cintilografia hepatica e aperfeicoamento de profissionais utilizando objeto simulador antropomorfico de figado em medicina nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Fernanda C.L.; Cunha, Cledison J.; Dullius, Marcos A.; Souza, Divanizia N., E-mail: divanizi@ufs.b [Universidade Federal de Sergipe (UFS), Aracaju, SE (Brazil)

    2011-10-26

    This paper evaluates hepatic scintigraphy procedures and the possibility of professional improvement in nuclear medicine by using three-dimension anthropomorphic simulators representative of adult human liver. For the conformation of anthropomorphic simulator, it was used gypsum and acrylic. Three simulator objects representing cirrhosis livers. The simulator were filled with {sup 99m}Tc diluted in water for the obtention of scintigraphic images. Tomographic images were analysed posterior and anterior of simulator representing an organ with more cirrhosis grade. It was observed that the simulators make possible the acquisition of images similar to the real images of liver with hepatic cirrhosis. The simulations of hemangiomas can contribute for continuous education of nuclear medicine professionals, as far the question of image formation is concerned, make possible the parameter study as, for example, the , matrix size energy window, zoom and counting statistic

  3. Use of CO2 as an angiographic contrast material in the diagnosis of acute hepatic hemorrhage a case report; Uso del CO2 como medio de contraste angiografico en el diagnostico de hemorragia hepatica aguda: a proposito de un caso

    Energy Technology Data Exchange (ETDEWEB)

    Gallardo, L.; Gorriz, E.; Pardo, M. D.; Reyes, R. [Hospital General de Gran Canaria. Dr. Negrin. Canarias (Spain)

    2001-07-01

    Selective abdominal arteriography with an iodinated contrast material is the method of choice for detecting the site of bleeding in patients with acute gastrointestinal hemorrhage in whom the results of endoscopic examination were negative CO{sub 2}, has been used successfully as a contrast material for arteriography of abdomen and lower limbs. We present the case of a patient in whom suspected gastrointestinal bleeding was detected more rapidly and reliably with co{sub 2} than with an iodinated contrast material. (Author) 18 Refs.

  4. NCBI nr-aa BLAST: CBRC-TTRU-01-1396 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1396 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.003 27% ...

  5. Individual Differences in Neurobehavioral Effects of Pyridostigmine

    Science.gov (United States)

    2001-02-01

    Technologies, Inc., Kansas City, Missouri (Unpublished) Honors research paper on Chemoattractants in Fasciola hepatica and snail hosts; Saint Mary...Disorders, Praxair Surface Technologies, Inc., Kansas City, Missouri . (Unpublished) Honors research paper on Chemoattractants in Fasciola ...Surface Technologies, Inc., Kansas City, Missouri (Unpublished) Honors research paper on Chemoattractants in Fasciola hepatica and snail hosts

  6. NCBI nr-aa BLAST: CBRC-EEUR-01-0379 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0379 ref|NP_066227.2|ND6_15504 NADH dehydrogenase subunit 6 [Fasciola ...hepatica] gb|AAG13155.2| NADH dehydrogenase subunit 6 [Fasciola hepatica] NP_066227.2 1.8 24% ...

  7. NCBI nr-aa BLAST: CBRC-TTRU-01-0835 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0835 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.17 26% ...

  8. NCBI nr-aa BLAST: CBRC-TTRU-01-0654 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0654 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.005 28% ...

  9. NCBI nr-aa BLAST: CBRC-TTRU-01-1086 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1086 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.010 25% ...

  10. NCBI nr-aa BLAST: CBRC-DNOV-01-0892 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0892 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.026 22% ...

  11. NCBI nr-aa BLAST: CBRC-DNOV-01-1259 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1259 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.049 29% ...

  12. NCBI nr-aa BLAST: CBRC-DNOV-01-1066 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1066 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.60 25% ...

  13. NCBI nr-aa BLAST: CBRC-TTRU-01-0655 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0655 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 4e-04 24% ...

  14. NCBI nr-aa BLAST: CBRC-TTRU-01-0742 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0742 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 3e-04 26% ...

  15. NCBI nr-aa BLAST: CBRC-TTRU-01-1355 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1355 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.002 23% ...

  16. NCBI nr-aa BLAST: CBRC-TTRU-01-1019 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1019 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.002 25% ...

  17. NCBI nr-aa BLAST: CBRC-TTRU-01-0451 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0451 ref|NP_066220.2|ND4_15504 NADH dehydrogenase subunit 4 [Fasciola ...hepatica] gb|AAG13148.2| NADH dehydrogenase subunit 4 [Fasciola hepatica] NP_066220.2 0.019 26% ...

  18. NCBI nr-aa BLAST: CBRC-DNOV-01-1978 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1978 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.23 24% ...

  19. NCBI nr-aa BLAST: CBRC-DNOV-01-0098 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0098 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.62 24% ...

  20. NCBI nr-aa BLAST: CBRC-TTRU-01-0748 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0748 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.015 25% ...

  1. NCBI nr-aa BLAST: CBRC-TTRU-01-0100 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0100 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 4e-04 25% ...

  2. NCBI nr-aa BLAST: CBRC-BTAU-01-0851 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-0851 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.024 24% ...

  3. NCBI nr-aa BLAST: CBRC-TTRU-01-1238 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1238 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.007 24% ...

  4. NCBI nr-aa BLAST: CBRC-TTRU-01-0370 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0370 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.001 26% ...

  5. NCBI nr-aa BLAST: CBRC-DNOV-01-0595 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0595 ref|NP_066222.2|ND2_15504 NADH dehydrogenase subunit 2 [Fasciola ...hepatica] gb|AAG13150.2| NADH dehydrogenase subunit 2 [Fasciola hepatica] NP_066222.2 0.39 25% ...

  6. A case of fascioliasis in common bile duct

    Energy Technology Data Exchange (ETDEWEB)

    Ham, Soo Youn; Park, Cheol Min; Chung, Kyu Byung; Lee, Chang Hong; Park, Seung Chul; Choi, Sang Yong; Lim, Han Jong [Korea University College of Medicine, Seoul (Korea, Republic of)

    1989-10-15

    A case of Fascioliasis of common bile duct is confirmed by visualization of adult fluke. Fascioliasis caused by Fasciola hepatica, is common parasitic disease in cattle and sheep. Human is an accidental host. ERCP demonstrated irregular linear conglomerated filling defects in common bile duct. Through surgical intervention, we found adult flukes of F. hepatica and adenomatous hyperplasia of common bile duct.

  7. A case of fascioliasis in common bile duct

    International Nuclear Information System (INIS)

    Ham, Soo Youn; Park, Cheol Min; Chung, Kyu Byung; Lee, Chang Hong; Park, Seung Chul; Choi, Sang Yong; Lim, Han Jong

    1989-01-01

    A case of Fascioliasis of common bile duct is confirmed by visualization of adult fluke. Fascioliasis caused by Fasciola hepatica, is common parasitic disease in cattle and sheep. Human is an accidental host. ERCP demonstrated irregular linear conglomerated filling defects in common bile duct. Through surgical intervention, we found adult flukes of F. hepatica and adenomatous hyperplasia of common bile duct

  8. Study of the influence of the temperature in the magnetic properties and in microstructure in the permanent magnets Pr-Fe-B-Nb-Co based obtained by hydrogen; Estudo da influencia da temperatura nas propriedades magneticas e na microestrutura nos imas permanentes a base de Pr-Fe-B-Nb-Co obtidos com hidrogenio

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Suelanny Carvalho da

    2007-07-01

    Fine magnetic powders were produced using the hydrogenation disproportionation desorption and recombination (HDDR) process. The first stage in this work involved an investigation of the effect of the Co content and range of desorption/ recombination temperatures between 800 and 900 deg C with the purpose of optimizing the HDDR treatment for Pr{sub 14}Fe{sub 80}B{sub 6} and Pr{sub 14}Fe{sub bal}Co{sub x}B{sub 6}Nb{sub 0,1} (x= 0, 4, 8, 10, 12, 16) alloys. The cast alloys were annealed at 1100 deg C for 20 hours for homogenization. The processing temperature (desorption/ recombination) affected the microstructure and magnetic properties of the bonded magnets. The alloy with low cobalt content (4 at.%) required the highest reaction temperature (880 deg C) to yield anisotropic bonded magnets. The optimum temperature for alloys with 8 at.% Co and 10 at.% Co were 840 deg C and 820 deg C, respectively. Alloys with high cobalt content (12 at.% and 16 at.%) were processed at 840 deg C. The optimum desorption temperature for achieving high anisotropy for Pr{sub 14}Fe{sub 80}B{sub 6} and Pr{sub 14}Fe{sub 79,9}B{sub 6}Nb{sub 0,1} was 820 deg C. The best remanence (862 mT) was achieved with the Pr{sub 14}Fe{sub 67,9}B{sub 6}Co{sub 12}Nb{sub 0,1} magnet, processed at 840 deg C. Each alloy required an optimum reaction temperature and exhibited a particular microstructure according to the composition. The second stage of the work involved the characterization, for each temperature, of the Pr{sub 14}Fe{sub 80}B{sub 6} HDDR powder processed using X-ray diffraction analysis. The samples of the HDDR material were studied by synchrotron radiation powder diffraction using the Rietveld method for cell refinement, phase quantification and crystallite sizes determination. Scanning electron microscopy has also been employed to reveal the morphology of the HDDR powder. (author)

  9. Milling condition effects on the Nd{sub 15} Fe{sub 77} B{sub 8} powder magnetic properties; Efeito das condicoes de moagem sobre as propriedades magneticas de po de Nd{sub 15} Fe{sub 77} B{sub 8}

    Energy Technology Data Exchange (ETDEWEB)

    Landgraf, Fernando J.G.; Missell, Frank P

    1993-12-31

    As a result of the first part of a study of the development of permanent magnets, an investigation of the effect of milling on the magnetic properties of the alloy Nd{sub 15} Fe{sub 77} B{sub 8} showed differences in efficiency between ball milling and vibration milling, as well as a dependence of the intrinsic coercive field on particle size. The maximum value of the coercive field was obtained for a particle size of 1.7 {mu}m in samples compacted without magnetic orientation. (author) 9 refs., 4 figs.

  10. Evaluation of the magnetic properties of hybrids MnFe{sub 2}O{sub 4}/SiO{sub 2} /chitosan aiming its application as biosensors; Avaliacao das propriedades magneticas de hibridos MnFe{sub 2}O{sub 4}/SiO{sub 2}/quitosana visando sua aplicacao como biosensores

    Energy Technology Data Exchange (ETDEWEB)

    Leal, E.; Santos, P.T. A.; Costa, F.M., E-mail: elvialeal@gmail.com [Universidade Federal de Campina Grande (LabSMaC/UFCG), PB (Brazil). Laboratorio de Sintese de Materiais Ceramicos; Barbosa, D.C. [Universidade Federal de Alagoas (UFAL), Maceio, AL (Brazil). Instituto de Quimica e Biotecnologia; Cornejo, D.R. [Universidade de Sao Paulo (USP), SP (Brazil). Instituto de Fisica; Kiminami, R.H.G.A. [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil). Departamento de Engenharia de Materiais

    2014-07-01

    Magnetic nanoparticles have potential application in biomedicine since their features allow a wide variety of applications, such as biosensors, drug carriers, destruction of tumor cells and magnetic separation of cells and proteins. Overlooking that, the proposal is to obtain the hybrid MnFe{sub 2}O{sub 4}/SiO{sub 2}/ chitosan, to evaluate it as its magnetic property, aiming to obtain a biocompatible hybrid for biological applications, such as, e.g., biosensors. The samples were analyzed by XRD, FTIR, SEM and magnetic measurements. The results revealed that the samples of pure MnFe{sub 2}O{sub 4}, silanized and with chitosan presented the formation of the spinel with crystallite sizes of 77, 80 and 79 nm, respectively. The FTIR spectra confirmed the presence of characteristic absorption bands of the spinel and groups present in silanol and chitosan, confirming the formation of the hybrid. The silane introduction kept the ferrimagnetic characteristic of the material and led to a slight increase in the saturation magnetization, going from 55 to 61 emu/g. (author)

  11. Fluorescein isothiocyanate labeled, magnetic nanoparticles conjugated D-penicillamine-anti-metadherin and in vitro evaluation on breast cancer cells; Avaliacao do isotiocianato de fluoresceina marcado, das nanoparticulas magneticas conjugadas da D-penicilamina antimetaderina e in vitro nas celulas do cancer de mama

    Energy Technology Data Exchange (ETDEWEB)

    Akca, Ozlet; Unak, Perihan; Medine, E. Ylker [Ege University (Turkey). Institute of Nuclear Sciences. Department of Nuclear Applications; Sakarya, Serhan [Adnan Menderes University (Turkey). ADUBILTEM Science and Technology Research and Development Center; Ozdemir, Caglar; Timur, Suna [Ege University (Turkey). Science Faculty. Department of Nuclear Applications

    2011-07-01

    Silane modified magnetic nanoparticles were prepared after capped with silica generated from the hydrolyzation of tetraethyl orthosilicate (TEOS). Amino silane (SG-Si900) was added to this solution for surface modification of silica coated magnetic particles. Finally, D-penicillamine (D-PA)-antimetadherin (anti-MTDH) was covalently linked to the amine group using glutaraldehyde as cross-linker. Magnetic nanoparticles were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), and atomic force microscopy (AFM). AFM results showed that particles are nearly monodisperse, and the average size of particles was 40 to 50 nm. An amino acid derivative D-PA was conjugated anti-MTDH, which results the increase of uptaking potential of a conjugated agent, labelled fluorescein isothiocyanate (FITC) and then conjugated to the magnetic nanoparticles. In vitro evaluation of the conjugated D-PA-anti-MTDH-FITC to magnetic nanoparticle was studied on MCF-7 breast cancer cell lines. Fluorescence microscopy images of cells after incubation of the sample were obtained to monitor the interaction of the sample with the cancerous cells. Incorporation on cells of FITC labeled and magnetic nanoparticles conjugated D-PA-anti-MTDH was found higher than FITC labeled D-PA-anti-MTDH. The results show that magnetic properties and application of magnetic field increased incorporation rates. The obtained D-PA-anti-MTDH-magnetic nanoparticles-FITC complex has been used for in vitro imaging of breast cancer cells. FITC labeled and magnetic nanoparticles conjugated D-PA-anti-MTDH may be useful as a new class of scintigraphic agents. Results of this study are sufficiently encouraging to bring about further evaluation of this and related compounds for ultraviolet magnetic resonance (UV-MR) dual imaging. (author)

  12. Thermal treatment of the Fe{sub 78} Si{sub 9} B{sub 13} alloy and the analysis of it magnetic properties through Moessbauer spectroscopy and Positronium annihilation; Tratamiento termico de la aleacion Fe{sub 78} Si{sub 9} B{sub 13} y el analisis de sus propiedades magneticas mediante Espectroscopia de Moessbauer y Aniquilacion de positronio

    Energy Technology Data Exchange (ETDEWEB)

    Lopez M, A

    2005-07-01

    The present work is divided in five chapters. In the first one a general vision of the amorphous alloys is given from antecedents, structure, obtaining methods, properties and problems that at the moment, focusing us in a certain moment to the iron base alloys and the anomalous problem of hardness that it presents the alloy Fe{sub 78}Si{sub 9}B{sub 13} like previously mention us. The second chapter tries on the basic theory of the techniques of Moessbauer spectroscopy and Positron Annihilation spectroscopy, used for the characterization of our alloy as well as the complementary technique of X-ray diffraction (XRD) to observe that the amorphous phase was even studying. The third chapter describes the experimental conditions that were used to study the alloy Fe{sub 78}Si{sub 9}B{sub 13} in each one of their thermal treatments. In the fourth chapter the obtained results and their discussion are presented. In the fifth chapter the conclusions to which were arrived after analyzing the results are presented. (Author)

  13. Study of the influence of PPO in the compatibility of the system PA6/PC/PPO through nuclear magnetic resonance II - Composition changes in the PA6/PC/PPO system; Estudo da afluencia do PPo na compatibilidade do sistema PA6/PC/PPO atraves de espectrometria de resonancia magnetica nuclear. II - Mudanca na composicao do sistema PA6/PC/PPO

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Dilma Alves; Oliveira, Clara Marize F.; Tavares, Maria Ines Bruno [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Macromoleculas; Menezes, Sonia M.C. [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisa

    1997-12-31

    This work aims to study the influence in the quantities of poly(propylene oxide) in the miscibility of the system 6-poly-amide/ poly - carbonate / poly (propylene oxide). NMR was used as chemical instrumental technique for the analysis. Results are presented 15 refs.

  14. Unambiguous assigning of the signals of the nuclear magnetic resonance spectra of {sup 1} H and {sup 13} C of monoterpenes using computational methods; Asignacion inequivoca de las senales del espectro de resonancia magnetica nuclear de {sup 1} H y {sup 13} C de monoterpenos empleando metodos computacionales

    Energy Technology Data Exchange (ETDEWEB)

    Cortes, F.; Cuevas, G.; Tenorio, J.; Rochin, A.L. [Universidad Nacional Autonoma de Mexico, Instituto de Quimica, A.P. 70213, 04510 Mexico D.F. (Mexico)

    2000-07-01

    Ab initio calculations, within the frame of Density Functional Theory were carried out on camphene and {alpha}-pinene. The {sup 1} H and {sup 13} C shifts were estimated according to the recently developed Sum-Over-States Density Functional Perturbation Theory (SOS-DFPT) as implemented in a modified deMon-KS program. The calculations not only reproduced the observed NMR chemical shifts, quantitatively in the case of {sup 1} H nuclei and qualitatively in the case of {sup 13} C nuclei, but also allow assigning unambiguously the signal on these spectra. (Author)

  15. Relations microstructure - magnetic properties - squareness factor of PrFeB and NdFeB sintered magnets prepared with hydrogen; Estudo das correlacoes entre microestrutura, propriedades magneticas e fator de quadratura em imas sinterizados de PrFeB e NdFeB processados com hidrogenio

    Energy Technology Data Exchange (ETDEWEB)

    Perigo, Elio Alberto

    2009-07-01

    In this work, it has firstly been evaluated the preparation of Pr{sub 16}Fe{sub 76}B{sub 8} sintered permanent magnets (% at.) by means of high-energy milling using a planetary ball mill. The influence of both milling speed and time has been verified. The best magnetic properties [J{sub R} = (1.02 {+-} 0.02) T, {mu}{sub 0J}H{sub c} = (1.42 {+-} 0.03) T and (BH){sub max} = (200 {+-} 4) kJm{sup -3}] have been found for a permanent magnet prepared with the magnetic alloy milled during 75 minutes using a rotational milling speed of 200 rpm. In order to improve the remanence, the hydrogen decrepitation process time has been reduced from 60 minutes to 2 minutes. In this case, it has been obtained a sintered magnet with J{sub R} = (1.14 {+-} 0.02) T, {mu}{sub 0J}H{sub c} = (1.44 {+-} 0.03) T and (BH){sub max} = (250 {+-} 5) kJm{sup -3} due to the improvement of crystallographic alignment of the hard magnetic phase. During such investigation, a new methodology to quantify the parameter has been developed. Subsequently, for the first time, a quantitative correlation between the microstructure and the squareness factor in anisotropic sintered RE{sub 16}Fe{sub 76}B{sub 8} (RE = Nd or Pr) magnets has been proposed. The presented expression utilizes the mean size, the mean elongation and the mean roundness of the hard magnetic grains as well as their respective standard deviations. The squareness factor can be improved with a microstructure with rounder grains and with a sharp grain size distribution. The grain size homogeneity is more important to enhance the squareness factor compared to grain shape homogeneity. Furthermore, it has also been verified that the annealing after sintering improves the grain shape homogeneity and the milling enhances the grain size homogeneity. Moreover, the effect of the temperature on the squareness factor of anisotropic sintered magnets has also been evaluated. Such parameter is mainly controlled by the sample's microstructure, in agreement with the proposed expression. Furthermore, a quantitative correlation between the maximum energy product and the squareness factor has been proposed. Experimental and calculated values have been compared and the discrepancy found has been, in general, inferior to 5%. At last, the minimum squareness factor value of sintered permanent magnets has also been evaluated. Isotropic samples should show the smallest squareness factor due to the reduced degree of crystallographic alignment. In general, for this kind of sample, 0.20 {<=} SF {<=} 0.30. (author)

  16. Study of amorphous semiconductors doped with rare earths (Gd and Er) and conducting polymers by EPR techniques and magnetic susceptibility; Estudo de semicondutores amorfos dopados com terras raras (Gd e Er) e de polimeros condutores atraves das tecnicas de RPE e susceptibilidade magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Sercheli, Mauricio da Silva

    1999-07-01

    This thesis involves the study of amorphous semiconductors and conducting polymers, which have been characterized by EPR and magnetic susceptibility measurements, and to a lesser extent by Raman spectroscopy and RBS. The semiconductors were studied using thin films of silicon doped with rare earth metals, e.g. erbium and gadolinium, which had their magnetic properties studied. Using these studies we could determine the state of valence of the rare earths as well as their concentrations in the silicon matrix. According to our results, the valence of the rare earth metal ions is 3+, and we were able to conclude that 4f electronic shells could not be used for the calculation of the conducting band in this system. Furthermore, the analysis of the data on the magnetic susceptibility of the Er{sup 3+} ion with cubic crystalline acting field, gave us the opportunity to estimate the overall splitting of their electronic states for the first time. The conducting polymers were studied using samples of poly(3-methylthiophene) doped with ClO{sub 4}{sup -}, which show a phase transition in the range of 230 K to 130 K. The electron paramagnetic resonance also gives important information on the crystallization, doping level and the presence of polarons or bipolarons in conducting polymers. (author)

  17. Synthesis and characterization of magnetic cobalt ferrite nanoparticles covered with 3-aminopropyltriethoxysilane for use as hybrid material in nano technology; Sintese e caracterizacao de nanoparticulas magneticas de ferrita de cobalto recobertas por 3-aminopropiltrietoxissilano para uso como material hibrido em nanotecnologia

    Energy Technology Data Exchange (ETDEWEB)

    Camilo, Ruth Luqueze

    2006-07-01

    Nowadays with the appear of nano science and nano technology, magnetic nanoparticles have been finding a variety of applications in the fields of biomedicine, diagnosis, molecular biology, biochemistry, catalysis, etc. The magnetic functionalized nanoparticles are constituted of a magnetic nucleus, involved by a polymeric layer with active sites, which ones could anchor metals or selective organic compounds. These nanoparticles are considered organic inorganic hybrid materials and have great interest as materials for commercial applications due to the specific properties. Among the important applications it can be mentioned: magneto hyperthermia treatment, drugs delivery in specific local of the body, molecular recognition, biosensors, enhancement of nuclear magnetic resonance images quality, etc. This work was developed in two parts: 1) the synthesis of the nucleus composed by superparamagnetic nanoparticles of cobalt ferrite and, 2) the recovering of nucleus by a polymeric bifunctional 3-aminopropyltriethoxysilane. The parameters studied in the first part of the research were: pH, hydroxide molar concentration, hydroxide type, reagent order of addition, reagent way of addition, speed of shake, metals initial concentrations, molar fraction of cobalt and thermal treatment. In the second part it was studied: pH, temperature, catalyst type, catalyst concentration, time of reaction, relation ratios of H{sub 2}O/silane, type of medium and the efficiency of the recovering regarding to pH. The products obtained were characterized using the following techniques X-ray powder diffraction (DRX), transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), spectroscopy of scatterbrained energy spectroscopy (DES), atomic emission spectroscopy (ICP-AES), thermogravimetric analysis (TGA/DTGA), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and magnetization curves (VSM). (author)

  18. Application of magnetic nanoparticle MnFe{sub 2}O{sub 4} type as a catalyst in esterification reaction; Aplicacao de nanoparticula magnetica do tipo MnFe{sub 2}O{sub 4} como catalisador em reacao de esterificacao

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, K.R. de O.; Barros, A.B. de S.; Moura, T.F.B. de; Vilar, E.; Dantas, J.; Costa, A. C. F. de M., E-mail: klebersonric@gmail.com [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Laboratorio de Sintese de Materiais Ceramicos

    2016-07-01

    The interest in obtaining renewable energy arouses the interest of researchers in the development of biofuels to replace conventional fuels. This work aimed to obtain magnetic nanoparticle MnFe{sub 2}O{sub 4} and evaluate their performance as a catalyst in esterification reaction to obtain biodiesel. The sample was synthesized through the combustion reaction and characterized by XRD, SEM and BET. The esterification reaction, the methyl ethyl route was conducted in a high pressure reactor at 180 ° C for 1 hour with oil molar ratio 1:12 alcohol with 2% catalyst. The results indicate the formation of the phase MnFe{sub 2}O{sub 4} and agglomerate in the form of irregular plate, with particles bound strongly to the surface of the agglomerates. The catalytic tests showed that sample was active for the reaction of esterification methyl ethyl route, with conversions of 52% and 48%, respectively. (author)

  19. A study of the process of Nd{sub 15} Fe{sub 77} B{sub 8} magnetic alloy preparation by calciothermic reduction diffusion (R/D); Estudo do processo de obtencao da liga magnetica Nd{sub 15} Fe{sub 77} B{sub 8} por reducao-difusao (R/D) calciotermica

    Energy Technology Data Exchange (ETDEWEB)

    Graca Guilherme, Eneida da; Paschoal, Jose O.A. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1992-12-31

    In this work the manufacturing of Nd{sub 15}, Fe{sub 77}, B{sub 8} alloy powder for high-performance permanent magnets by reduction-diffusion process was investigated. The effect of variables such as the excess amount of neodymium oxide, the removal of calcium oxide by selective leaching and milling of alloy was examined. (author). 8 refs., 8 figs., 5 tab.s.

  20. Comparative study between the Spin-echo and 3-D fast imaging techniques in the Knee evaluation with magnetic resonance. Estudio comparativo entre las tecnicas de Spin-Eco ecogradiente 3D, en la evaluacion de la rodilla con resonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Oleaga Zufiria, L.; Ibaez Zubiarrain, A.; Grande Icaran, J.; Vela Martin, A.C.; Cintora Leon, E.; Grau Garcia, M.; Grande Icaran, D. (Hospital Civil de Bilbao. Servicio de Radiodiagnostico. Bilbao (Spain))

    1993-01-01

    We have carried out a retrospective analysis of the results of magnetic resonance (MR) studies in 20 patients, comparing two different sequences. We compared a 2-D spin-echo (SE2D) sequence with a 3-D fast imaging with steady-state precession (FISP3D) sequence in the attempt to compare the reliability of each in the detection of knee injuries. Arthroscopy was employed as a control technique. Our study revealed no statistically significant difference between the two sequences, although the overall sensitivity for the detection of meniscal lesions was slightly greater with the FISP3D sequence; however, the reliability in the detection of ruptures of the posterior cruciate ligament is less with this sequence than with the SE2D sequence. Both sequences showed very low sensitivity in the detection of hyaline cartilage injuries. (Author) 14 refs.

  1. Control optimo de par para maquinas SynRM aplicadas a vehiculo electrico

    OpenAIRE

    Trancho, Elena; Ibarra, E.; Arias, A.; Kortabarria, I.

    2017-01-01

    Las maquinas de reluctancia sincrona asistidas por imanes estan atrayendo un considerable interes como alternativa a las maquinas sıncronas de imanes permanentes para su uso en sistemas de propulsion de vehıculos electricos. El control optimo de estas maquinas (incluyendo operacion en debilitamiento de campo) puede resultar complejo, ya que estas son, por lo general, muy sensibles al fenomeno de la saturacion magnetica. En este articulo se trata su control, desde los reguladores hasta el prec...

  2. Il nucleo terrestre: il cuore magnetico della Terra

    OpenAIRE

    De Santis, A.

    2006-01-01

    Il campo magnetico terrestre è una proprietà intrinseca del nostro pianeta e di altri oggetti del sistema solare. Il Sole stesso possiede un forte campo magnetico che si inverte quasi ciclicamente ogni 10-11 anni; tale comportamento è visibile attraverso la medesima ciclicità delle macchie solari che denotano sulla superficie l’intensa attività magnetica della nostra stella. Il campo magnetico terrestre è importantissimo per la vita sulla Terra. Esso protegge il pianeta dalle p...

  3. Radioimmunoassay in the diagnosis of fascioliasis

    Energy Technology Data Exchange (ETDEWEB)

    Tomanek, J; Willomitzer, J; Chroustova, E [Veterinary Research Inst., Brno (Czechoslovakia)

    1978-06-30

    The objective of the study was to develop a sensitive and specific radioimmunoassay (RIA) method for the diagnosis of F. hepatica infection that would be convenient to use in examining large numbers of samples. The main problem was the preparation of a suitable antigen or specific fraction, since the crude extract of adult Fasciola hepatica is a heterogeneous mixture of somatic, metabolic and host substances with possible nonspecific antigenic properties. The antigen was a protein fraction separated from crude extract of adult F. hepatica by gel filtration and labelled with /sup 125/iodine.

  4. Radioimmunoassay in the diagnosis of fascioliasis

    International Nuclear Information System (INIS)

    Tomanek, J.; Willomitzer, J.; Chroustova, E.

    1978-01-01

    The objective of the study was to develop a sensitive and specific radioimmunoassay (RIA) method for the diagnosis of F. hepatica infection that would be convenient to use in examining large numbers of samples. The main problem was the preparation of a suitable antigen or specific fraction, since the crude extract of adult Fasciola hepatica is a heterogeneous mixture of somatic, metabolic and host substances with possible nonspecific antigenic properties. The antigen was a protein fraction separated from crude extract of adult F. hepatica by gel filtration and labelled with 125 iodine. (T.I.)

  5. Structural and Magnetic Studies on Iron Oxide Nanoparticles in Hybrid Matrices =

    Science.gov (United States)

    Silva, Nuno Joao de Oliveira

    Este trabalho aborda algumas propriedades magneticas e estruturais de nanoparticulas de oxidos e oxidos-hidroxidos de ferro crescidos em matrizes hibridas orgânicas-inorgânicas. As matrizes hibridas, denominadas di-ureasils e obtidas pelo processo sol-gel, sao compostas por uma rede siliciosa ligada covalentemente por pontes ureia a cadeias orgânicas de diferente peso molecular. A estrutura local dos di-ureasils nao dopados esta modelada como grupos de dominios siliciosos com dimensoes nanometricas, estruturalmente correlacionados no seio de uma matriz rica em polimero. Neste trabalho mostra-se que os di-ureasils permitem o crescimento controlado de oxidos e oxidos-hidroxidos de ferro, incluindo a magnetite, maguemite, oxihidroxinitrato de ferro e ferrihidrite. O crescimento das nanoparticulas de ferrihidrite da-se em condicoes acidas a superficie dos dominios siliciosos, junto aos grupos carbonilo, que funcionam como pontos de nucleacao. Desse modo da-se uma nucleacao heterogenea, onde o tamanho das nanoparticulas depende da concentracao de ferro (entre 1 e 6% em massa), sendo a concentracao de particulas constante. As propriedades magneticas das nanoparticulas de ferrihidrite revelam a existencia de interaccoes antiferromagneticas e de momentos descompensados. A contribuicao destas duas componentes nas curvas de magnetizacao em funcao do campo magnetico pode ser separada usando um metodo aqui proposto, o que permite um adequado estudo da evolucao do momento magnetico com a temperatura. O estudo das propriedades magneticas dinâmicas das particulas de ferrihidrite, atraves de susceptibilidade ac, medidas de relaxacao e medidas de efeito Mossbauer, permitiu estudar a evolucao das interaccoes dipolares em funcao da concentracao de ferro, bem como determinar a distribuicao de barreiras de energia de anisotropia no caso em que essas interaccoes sao desprezaveis. E apresentado um novo metodo para comparacao desta distribuicao com a distribuicao de tamanhos, que

  6. Spin-Transfer-Torque Nano-Oscillators: Fabrication, Characterization and Dynamics

    Science.gov (United States)

    Costa, Jose Diogo Teixeira Barbosa

    Este trabalho aborda algumas propriedades magneticas e estruturais de nanoparticulas de oxidos e oxidos-hidroxidos de ferro crescidos em matrizes hibridas orgânicas-inorgânicas. As matrizes hibridas, denominadas di-ureasils e obtidas pelo processo sol-gel, sao compostas por uma rede siliciosa ligada covalentemente por pontes ureia a cadeias orgânicas de diferente peso molecular. A estrutura local dos di-ureasils nao dopados esta modelada como grupos de dominios siliciosos com dimensoes nanometricas, estruturalmente correlacionados no seio de uma matriz rica em polimero. Neste trabalho mostra-se que os di-ureasils permitem o crescimento controlado de oxidos e oxidos-hidroxidos de ferro, incluindo a magnetite, maguemite, oxihidroxinitrato de ferro e ferrihidrite. O crescimento das nanoparticulas de ferrihidrite da-se em condicoes acidas a superficie dos dominios siliciosos, junto aos grupos carbonilo, que funcionam como pontos de nucleacao. Desse modo da-se uma nucleacao heterogenea, onde o tamanho das nanoparticulas depende da concentracao de ferro (entre 1 e 6% em massa), sendo a concentracao de particulas constante. As propriedades magneticas das nanoparticulas de ferrihidrite revelam a existencia de interaccoes antiferromagneticas e de momentos descompensados. A contribuicao destas duas componentes nas curvas de magnetizacao em funcao do campo magnetico pode ser separada usando um metodo aqui proposto, o que permite um adequado estudo da evolucao do momento magnetico com a temperatura. O estudo das propriedades magneticas dinâmicas das particulas de ferrihidrite, atraves de susceptibilidade ac, medidas de relaxacao e medidas de efeito Mossbauer, permitiu estudar a evolucao das interaccoes dipolares em funcao da concentracao de ferro, bem como determinar a distribuicao de barreiras de energia de anisotropia no caso em que essas interaccoes sao desprezaveis. E apresentado um novo metodo para comparacao desta distribuicao com a distribuicao de tamanhos, que

  7. [A case of fascioliasis in the intrahepatic duct with concurrent clonochiasis].

    Science.gov (United States)

    Kim, Seung-Hyun; Jung, Chang-Kil; Her, Jin; Hur, Ki-Hwan; Choi, Jae-Hyuc; Kang, Kee-Hoon; Hwang, Chan-Hee

    2014-11-01

    The main causes of biliary obstruction are stones and cancers. Fascioliasis is a very rare case which causes biliary obstruction. Fascioliasis is a zoonosis caused by Fasciola hepatica which infects herbivores like sheep and cattle. F. hepatica lives in the biliary system or the liver parenchyma of a host. In Korea, the occurrence of this infection in human is very rare and only few cases have been reported. A 32-year-old male presented with upper abdominal pain and jaundice. His laboratory finding revealed elevated liver transaminases. Abdomen CT scan showed mild left intrahepatic bile duct dilatation. On ERCP, adult F. hepatica worms were found and were thus removed. Concurrently, clonorchiasis was diagnosed by stool exam and serologic enzyme-linked immunosorbent assay test. Clonorchiasis was treated with praziquantel. Herein, we report a case of intrahepatic bile duct dilatation due to F. hepatica infection with concurrent Clonorchis sinensis infestation.

  8. Hepáticas del Parque Natural Municipal “Robledales de Tipacoque”, Boyacá-Colombia

    OpenAIRE

    Diana Lucía Vargas-Rojas1 , María Eugenia Morales-Puentes2

    2014-01-01

    Abstract In high mountain ecosystems, Hepaticae is prominent and are essential to regulate and maintain hydrological cycles; they also contribute to nutrient recycling. We sampled five different substrates (decomposing matter, tree base, bare soil, rocks and flush root) of the "Robledales de Tipacoque" Natural Park and recorded 52 species of Hepaticae belonging to 21 genera and 12 families. The families having the highest number of species were the Plagiochilaceae with 22 (40.7%) and Lejeu...

  9. Hepáticas del Parque Natural Municipal “Robledales de Tipacoque”, Boyacá-Colombia

    OpenAIRE

    Vargas-Rojas, Diana Lucía; Universidad federal de viçosa; Morales-Puentes, María Eugenia

    2014-01-01

    In high mountain ecosystems, Hepaticae is prominent and are essential to regulate and maintain hydrological cycles; they also contribute to nutrient recycling. We sampled five different substrates (decomposing matter, tree base, bare soil, rocks and flush root) of the "Robledales de Tipacoque" Natural Park and recorded 52 species of Hepaticae belonging to 21 genera and 12 families. The families having the highest number of species were the Plagiochilaceae with 22 (40.7%) and Lejeuneaceae with...

  10. Monitoring of Fasciola Species Contamination in Water Dropwort by cox1 Mitochondrial and ITS-2 rDNA Sequencing Analysis.

    Science.gov (United States)

    Choi, In-Wook; Kim, Hwang-Yong; Quan, Juan-Hua; Ryu, Jae-Gee; Sun, Rubing; Lee, Young-Ha

    2015-10-01

    Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.

  11. Novel methods for the molecular discrimination of Fasciola spp. on the basis of nuclear protein-coding genes.

    Science.gov (United States)

    Shoriki, Takuya; Ichikawa-Seki, Madoka; Suganuma, Keisuke; Naito, Ikunori; Hayashi, Kei; Nakao, Minoru; Aita, Junya; Mohanta, Uday Kumar; Inoue, Noboru; Murakami, Kenji; Itagaki, Tadashi

    2016-06-01

    Fasciolosis is an economically important disease of livestock caused by Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola flukes. The aspermic Fasciola flukes have been discriminated morphologically from the two other species by the absence of sperm in their seminal vesicles. To date, the molecular discrimination of F. hepatica and F. gigantica has relied on the nucleotide sequences of the internal transcribed spacer 1 (ITS1) region. However, ITS1 genotypes of aspermic Fasciola flukes cannot be clearly differentiated from those of F. hepatica and F. gigantica. Therefore, more precise and robust methods are required to discriminate Fasciola spp. In this study, we developed PCR restriction fragment length polymorphism and multiplex PCR methods to discriminate F. hepatica, F. gigantica, and aspermic Fasciola flukes on the basis of the nuclear protein-coding genes, phosphoenolpyruvate carboxykinase and DNA polymerase delta, which are single locus genes in most eukaryotes. All aspermic Fasciola flukes used in this study had mixed fragment pattern of F. hepatica and F. gigantica for both of these genes, suggesting that the flukes are descended through hybridization between the two species. These molecular methods will facilitate the identification of F. hepatica, F. gigantica, and aspermic Fasciola flukes, and will also prove useful in etiological studies of fasciolosis. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  12. Seroprevalence and Risk Factors of Fascioliasis in Yaks, Bos grunniens, from Three Counties of Gansu Province, China.

    Science.gov (United States)

    Zhang, Xiao-Xuan; Feng, Sheng-Yong; Ma, Jian-Gang; Zheng, Wen-Bin; Yin, Ming-Yang; Qin, Si-Yuan; Zhou, Dong-Hui; Zhao, Quan; Zhu, Xing-Quan

    2017-02-01

    The aim of this study was to determine the seroprevalence and risk factors of fascioliasis in yaks, Bos grunniens , from 3 counties of Gansu Province in China. A total of 1,584 serum samples, including 974 samples from white yaks from Tianzhu, 464 from black yaks from Maqu, and 146 from black yaks from Luqu County, were collected and analyzed using ELISA to detect IgG antibodies against Fasciola hepatica . The overall F. hepatica seroprevalence was 28.7% (454/1,584), with 29.2% in white yaks (284/974) and 27.9% in black yaks (170/610). The seroprevalence of F. hepatica in yaks from Tianzhu, Luqu, and Maqu was 29.2%, 22.6%, and 29.5%, respectively. Female yaks (30.9%) had higher F. hepatica seroprevalence than male yaks (23.4%). Also, F. hepatica seroprevalence varied by different age group from 24.1% to 33.8%. Further, the seroprevalence ranged from 21.8% to 39.1% over different seasons. Interestingly, the season and age of yaks were associated with F. hepatica infection in yaks in the investigated areas. These findings provided a basis for further studies on this disease in yaks from 3 counties of Gansu Province in northwestern China, which may ultimately support the development of effective control strategies of fascioliasis in these areas.

  13. Synthesis and characterization of magnetic nanoparticles of oxides for dual MnFe{sub 2}O{sub 4} bioseparation, stabilized in fatty acid and the system chitosan - Eu(TTA){sub 3}(TPPO){sub 2}. Studies on the influence of doping with Gd{sup 3+}, Tb{sup 3+}, Ho{sup 3+} e Eu{sup 3+} in structural and magnetic properties; Sintese e caracterizacao de nanoparticulas magneticas de oxidos duplos de MnFe{sub 2}O{sub 4} para biosseparacao, estabilizadas em acido graxo e recobertas pelo sistema quitosana - Eu(TTA){sub 3}(TPPO){sub 2}. Estudo da influencia da dopagem com Gd{sup 3+}, Tb{sup 3+}, Ho{sup 3+} e Eu{sup 3+} nas propriedades estruturais e magneticas

    Energy Technology Data Exchange (ETDEWEB)

    Kovacs, Thelma Antunes Rodrigues

    2014-07-01

    This work was synthesized and characterized ferrite magnetic nanoparticles manganese, using the chemical coprecipitation method. By varying the heating time under 98°C (0, 10,20,40,60 3 80 minutes), the molar percentage of doping (1, 3, 5, 7, and 10%), gadolinium, europium, terbium and holmium. Magnetic ferrite nanoparticles and manganese ferrite doped with manganese were synthesized by coprecipitation method starting with chloride solutions of metals (iron (III), manganese (II), europium (III), gadolinium (III), terbium (III) and holmium (III)) and NaOH 5mol.L{sup -1} as precipitating agent. The magnetic nanoparticles were characterized by scanning electron microscopy, infrared spectroscopy, X-ray diffraction, magnetization curves, and thermal analysis. Most of manganese ferrite particles showed superparamagnetic behavior. After the characterization it was found that the samples synthesized manganese ferrite with more than 40 minutes heating time, crystal structure showed the characteristic pattern of the inverted manganese ferrite spinel type. The stabilization of the samples in oleic acid nanoparticles produced with a hydrophobic outer layer and facilitated by coating chitosan biopolymer, since this has a positive charge. Among the doped samples there was no significant change in the magnetic behavior. Several techniques for characterizing these materials have been used such as X-ray diffraction spectrum in the infrared region, magnetization curves and thermal analysis. The resins were tested as magnetic material for the separation of biological materials. In this paper, are used as biological targets separation of bovine serum albumin. (author)

  14. Characterization of magnetic nano particles of CoFe{sub 2}O{sub 4} and CoZnFe{sub 2}O{sub 4} prepared by the chemical co-precipitation method; Caracterizacion de nanoparticulas magneticas de CoFe{sub 2}O{sub 4} y CoZnFe{sub 2}O{sub 4} preparadas por el metodo de coprecipitacion quimica

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, J.; Zambrano, G.; Gomez, M. E. [Universidad del Valle, Departamento de Fisica, Laboratorio de Peliculas Delgadas, Ciudad Universitaria Melendez, 25360 Cali (Colombia); Prieto, P. [Universidad del Valle, Centro de Excelencia en Nuevos Materiales, Ciudad Universitaria Melendez, 25360 Cali (Colombia); Espinoza B, F. J., E-mail: javierlo21@gmail.com [IPN, Centro de Investigacion y de Estudios Avanzados, Unidad Queretaro, Libramiento Norponiente No. 2000, Fracc. Real de Juriquilla, 76230 Queretaro (Mexico)

    2012-07-01

    Magnetic cobalt ferrite nanoparticles of CoFe{sub 2}O{sub 4} and CoZnFe{sub 2}O{sub 4} were prepared by co-precipitation technique from aqueous salt solutions of Co (II), ZnSO{sub 4} and Fe (III), in an alkaline medium. CoFe{sub 2}O{sub 4} powder samples were structurally characterized by X-ray diffraction, showing the presence of the most intense peat at 2{theta} = 413928{sup o} (Co K{alpha}1) corresponding to the (311) crystallographic orientation of the CoFe{sub 2}O{sub 4} spinel phase. The mean size of the crystalline of CoFe{sub 2}O{sub 4} and CoZnFe{sub 2}O{sub 4} nanoparticles determined from the full width at half maximum of the strongest reflection of the (311) peak by using the Scherrer approximation was calculated to be 11.4 and 7.0 ({+-} o.2) nm, respectively. Transmission electron microscopy studies permitted determining nanoparticle size of CoZnFe{sub 2}O{sub 4}. Fourier transform infrared spectroscopy was used to confirm the formation of Fe-O bonds, allowing identifying the presence of ferrite spinel structure. Magnetic properties were investigated with the aid of a vibrating sample magnetometer at room temperature Herein, the sample showed superparamagnetic behavior, determined by the hysteresis loop finally, due to the hysteresis loop of the CoZnFe{sub 2}O{sub 4} is very small, our magnetic nanoparticles can be considered as a soft magnetic material. These magnetic nanoparticles have interesting technological applications in biomedicine given their biocompatibility, in nano technology, and in ferro fluid preparation. (Author)

  15. Study of the effect of dilution on the microstructural and magnetic properties of Mn{sub 0.65}Zn{sub 0.35}Fe{sub 2}O{sub 4} ferrites by combustion reaction; Estudo do efeito da diluicao nas propriedades microestruturais e magneticas de ferritas Mn{sub 0.65}Zn{sub 0.35}Fe{sub 2}O{sub 4} por reacao de combustao

    Energy Technology Data Exchange (ETDEWEB)