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Sample records for resistance measurements samples

  1. Accurate microfour-point probe sheet resistance measurements on small samples

    DEFF Research Database (Denmark)

    Thorsteinsson, Sune; Wang, Fei; Petersen, Dirch Hjorth

    2009-01-01

    We show that accurate sheet resistance measurements on small samples may be performed using microfour-point probes without applying correction factors. Using dual configuration measurements, the sheet resistance may be extracted with high accuracy when the microfour-point probes are in proximity...... of a mirror plane on small samples with dimensions of a few times the probe pitch. We calculate theoretically the size of the “sweet spot,” where sufficiently accurate sheet resistances result and show that even for very small samples it is feasible to do correction free extraction of the sheet resistance...

  2. Inter-strand resistance measurements in the termination of the ITER SULTAN samples

    International Nuclear Information System (INIS)

    Cau, F; Bruzzone, P

    2009-01-01

    In cabled conductors a perfect uniformity of the current among the strands is hardly reached, due to the non-homogeneity of the contact resistance distribution between the strands and the copper of the electrical terminations. In the case of large current unbalance, the overloaded strands hit the critical surface at high field early, developing a current sharing voltage, which drives the redistribution of the current, mainly in the electrical terminations where the inter-strand resistance is lower than in the high field conductor. If the inter-strand resistance in the termination is low, the voltage levels are sufficiently low to allow an effective redistribution of the current to the less loaded strands. The inter-strand resistance of three different termination layouts of ITER short length samples is measured to make a database available which can be used to qualify the layout of the joints and their capability of redistributing the current among the strands.

  3. SQUID based resistance bridge for shot noise measurement on low impedance samples

    OpenAIRE

    Jehl, X.; Payet-Burin, P.; Baraduc, C.; Calemczuk, R.; Sanquer, M.

    1999-01-01

    We present a resistance bridge which uses a SQUID to measure the shot noise in low impedance samples. The experimental requirements are high DC bias currents (typically 10mA) together with high AC sensitivity (pA/VHz). This system is used to investigate the shot noise in Superconductor/Normal/Superconductor junctions where Andreev reflection enhanced shot noise is expected. Because our setup has an intrinsic noise much smaller than the thermal noise of the resistance bridge at 4.2K, reliable ...

  4. A New Instrument to Measure the Surface Resistance of Superconducting Samples at 400 MHz

    CERN Document Server

    Mahner, E; Chiaveri, Enrico; Häbel, E; Tessier, J M

    2003-01-01

    A 400-MHz niobium quadrupole resonator has been manufactured to study the rf properties of superconducting bulk and thin film samples at low temperatures. We describe the apparatus, i.e. the construction of the resonator, field calculations with MAFIA, and the experimental procedure. In first validation tests the surface resistance Rs of a reactor-grade bulk niobium sample as a function of temperature and applied rf field has been investigated by using a calorimetric "rf-dc-compensation" method. A critical temperature Tc = 9.15 ± 0.02 K, a thermal conductivity λ (4.2 K) = 6.9 ± 0.7 W/mK, a residual resistance Rres = 19.0 ± 0.3 nΩ and a superconducting energy gap of ∆/kBTc = 1.82 ± 0.01 have been measured. At 4.2 K we achieved a calorimetric detection limit for Rs of 0.16 nΩ at a peak field of 25 mT.

  5. Electromigration early resistance increase measurements

    NARCIS (Netherlands)

    Niehof, J.; Flinn, P.A.; Maloney, T.J.

    1993-01-01

    An early resistance change measurement set-up, using an AC bridge technique, has been developed, and measurements have been performed. Large sample-to-sample variations occur. The characteristic time for the resistance change curve is shorter for resistance increase (under current stress) than for

  6. Numerical analysis of the DC performance of ITER TF samples with different cabling pattern based on resistance measurements on terminations

    Science.gov (United States)

    van Lanen, E. P. A.; Nijhuis, A.

    2011-08-01

    The DC transport properties of ITER TF conductors are analyzed in the SULTAN facility, but their associated current sharing temperature (Tcs) can give scope for different interpretations. To extract the conductor's pure performance during such short sample tests requires a detailed quantitative model such as JackPot, for which as many as possible input parameters are based on experimentally verified data. The crucial interstrand contact resistance data of the TFPRO-2 and TFJA-3 samples, which have a different cabling and joint layout, has recently become available. With only three parameters for JackPot's joint model, we were able to find a good match between the measured and simulated interstrand resistances for all terminations, despite their different layout. For Nb3Sn conductors, the axial strain is the only free model parameter left for matching the simulations with SULTAN Tcs tests. We were able to find a match with the measured voltage-temperature characteristic for all but one conductor. The simulation results indicate that the termination layout can be the cause of lower sample performance due to current imbalance. The outcome of this analysis also confirms that transverse load degradation can be significantly mitigated by finding an optimal set of cable twist pitches.

  7. A reversible, non-invasive method for airway resistance measurements and bronchoalveolar lavage fluid sampling in mice.

    Science.gov (United States)

    Polikepahad, Sumanth; Barranco, Wade T; Porter, Paul; Anderson, Bruce; Kheradmand, Farrah; Corry, David B

    2010-04-13

    Airway hyperreactivity (AHR) measurements and bronchoalveolar lavage (BAL) fluid sampling are essential to experimental asthma models, but repeated procedures to obtain such measurements in the same animal are generally not feasible. Here, we demonstrate protocols for obtaining from mice repeated measurements of AHR and bronchoalveolar lavage fluid samples. Mice were challenged intranasally seven times over 14 days with a potent allergen or sham treated. Prior to the initial challenge, and within 24 hours following each intranasal challenge, the same animals were anesthetized, orally intubated and mechanically ventilated. AHR, assessed by comparing dose response curves of respiratory system resistance (RRS) induced by increasing intravenous doses of acetylcholine (Ach) chloride between sham and allergen-challenged animals, were determined. Afterwards, and via the same intubation, the left lung was lavaged so that differential enumeration of airway cells could be performed. These studies reveal that repeated measurements of AHR and BAL fluid collection are possible from the same animals and that maximal airway hyperresponsiveness and airway eosinophilia are achieved within 7-10 days of initiating allergen challenge. This novel technique significantly reduces the number of mice required for longitudinal experimentation and is applicable to diverse rodent species, disease models and airway physiology instruments.

  8. AC resistance measuring instrument

    Science.gov (United States)

    Hof, P.J.

    1983-10-04

    An auto-ranging AC resistance measuring instrument for remote measurement of the resistance of an electrical device or circuit connected to the instrument includes a signal generator which generates an AC excitation signal for application to a load, including the device and the transmission line, a monitoring circuit which provides a digitally encoded signal representing the voltage across the load, and a microprocessor which operates under program control to provide an auto-ranging function by which range resistance is connected in circuit with the load to limit the load voltage to an acceptable range for the instrument, and an auto-compensating function by which compensating capacitance is connected in shunt with the range resistance to compensate for the effects of line capacitance. After the auto-ranging and auto-compensation functions are complete, the microprocessor calculates the resistance of the load from the selected range resistance, the excitation signal, and the load voltage signal, and displays of the measured resistance on a digital display of the instrument. 8 figs.

  9. Tritium sampling and measurement

    International Nuclear Information System (INIS)

    Wood, M.J.; McElroy, R.G.; Surette, R.A.; Brown, R.M.

    1993-01-01

    Current methods for sampling and measuring tritium are described. Although the basic techniques have not changed significantly over the last 10 y, there have been several notable improvements in tritium measurement instrumentation. The design and quality of commercial ion-chamber-based and gas-flow-proportional-counter-based tritium monitors for tritium-in-air have improved, an indirect result of fusion-related research in the 1980s. For tritium-in-water analysis, commercial low-level liquid scintillation spectrometers capable of detecting tritium-in-water concentrations as low as 0.65 Bq L-1 for counting times of 500 min are available. The most sensitive method for tritium-in-water analysis is still 3He mass spectrometry. Concentrations as low as 0.35 mBq L-1 can be detected with current equipment. Passive tritium-oxide-in-air samplers are now being used for workplace monitoring and even in some environmental sampling applications. The reliability, convenience, and low cost of passive tritium-oxide-in-air samplers make them attractive options for many monitoring applications. Airflow proportional counters currently under development look promising for measuring tritium-in-air in the presence of high gamma and/or noble gas backgrounds. However, these detectors are currently limited by their poor performance in humidities over 30%. 133 refs

  10. Electrical resistivity measurements to predict abrasion resistance of ...

    Indian Academy of Sciences (India)

    WINTEC

    39⋅0. 2⋅29. 4⋅04. Figure 1. Resistivity measurement system. pore fluid salinity, pore fluid saturation, temperature and pressure were kept the same. Resistivity measurements were performed on cylindri- cal samples of 54⋅4 mm diameter and ~ 50 mm length. Axial end surfaces of the samples were ground flat and parallel.

  11. Electrical resistivity measurement to predict uniaxial compressive ...

    Indian Academy of Sciences (India)

    Abstract. Electrical resistivity values of 12 different igneous rocks were measured on core samples using a resistivity meter in the laboratory. The resistivity tests were conducted on the samples fully saturated with brine (NaCl solution) and the uniaxial compressive strength (UCS), Brazilian tensile strength, density and.

  12. Perceptions of antimicrobial usage, antimicrobial resistance and policy measures to reduce antimicrobial usage in convenient samples of Belgian, French, German, Swedish and Swiss pig farmers.

    Science.gov (United States)

    Visschers, V H M; Backhans, A; Collineau, L; Iten, D; Loesken, S; Postma, M; Belloc, C; Dewulf, J; Emanuelson, U; Beilage, E Grosse; Siegrist, M; Sjölund, M; Stärk, K D C

    2015-04-01

    We conducted a survey among convenient samples of pig farmers (N=281) in Belgium, France, Germany, Sweden and Switzerland. We identified some significant differences among the five investigated countries (independent variable) regarding farmers' antimicrobial usage compared to their own country and worries related to pig farming (dependent variables), but most of the differences were rather small. In general, farmers perceived their own antimicrobial usage to be lower than that of their peers in the same country and lower than or similar to that of farmers from other countries. This may be a consequence of our convenience sample, resulting in self-selection of highly motivated farmers. Farmers were significantly more worried about financial/legal issues than about antimicrobial resistance. They believed that a reduction in revenues for slaughter pigs treated with a large amount of antimicrobials would have the most impact on reduced antimicrobial usage in their country. Further, farmers who were more worried about antimicrobial resistance and who estimated their own antimicrobial usage as lower than their fellow countrymen, perceived more impact from policy measures on the reduction of antimicrobials. Our results indicated that the same policy measures can be applied to reduce antimicrobial usage in pig farming in all five countries. Moreover, it seems worthwhile to increase pig farmers' awareness of the threat of antimicrobial resistance and its relation to antimicrobial usage; not only because pig farmers appeared little worried about antimicrobial usage but also because it affected farmers' perception of policy measures to reduce antimicrobial usage. Our samples were not representative for the national pig farmer populations. Further research is therefore needed to examine to what extent our findings can be generalised to these populations and to farmers in other countries. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Time-lapse resistivity measurements combined with soil water sampling to characterize solute movement in the unsaturated zone at Oslo airport, Gardermoen

    Science.gov (United States)

    Bloem, E.; French, H. K.; Binley, A.; Schotanus, D.; Eggen, G.

    2010-12-01

    Pollution of soils is a widespread problem and is an important part of the still to be implemented Soil Directive (EU). To improve risk assessment, monitoring, and treatment strategies for natural attenuation, we require a better understanding of the effect of soil heterogeneity on contaminant movement and methods for monitoring the effects of this heterogeneity at contaminated sites. Geophysical methods provide indirect measurements of subsurface properties over larger volumes than tradition techniques, and are potentially cost-efficient. However, the usefulness of any individual set of geophysical measurements (akin to a snapshot at one point in time) is severely limited by the problem of non-uniqueness or ambiguity when used to study contaminated sites, where the attendant processes vary in space and time. To make progress on soil contamination assessment and site characterization there is a strong need to integrate quasi field-scale, extensively instrumented tools, such as the multi-compartment sampler, with non-invasive (geophysical) and invasive (soil sampling, soil water sampling) methods. We illustrate this approach in an application to solute transport at Oslo airport, Norway. The impact of annual infiltration of large quantities of de-icing chemicals at Oslo airport, Gardermoen, represents common challenge for all airports with winter frost. It is also similar to the challenge posed by de-icing salt application along roads. At the research field station at Gardermoen, a degradable de-icing chemical and an inactive tracer were added to the snow cover prior to snowmelt and to the surface during an irrigation experiment performed after the snowmelt. In order to link geophysical measurements to solute transport processes in the unsaturated zone, time-lapse cross borehole resistivity as well as surface resistivity measurements were conducted at the same time as soil water samples were extracted. Measurements of soil temperature, and tension were also carried

  14. Surface resistivity measurement of plasma treated polymers

    International Nuclear Information System (INIS)

    Simon, D.; Pigram, P.J.; Liesegang, J.

    2000-01-01

    Full text: Resistivity of insulators is an important property of materials used within the integrated circuit and packaging industries. The measurement of electrical resistivity of insulator materials in the surface region in this work is interpreted through observations of surface charge decay. A self-field driven and diffusion charge transport theory is used to model the process and resistivity values obtained computationally. Data for the charge decay of surface charged samples are collected by suspending them inside a coaxial cylinder connected to an electrometer. Samples used have been low density polyethylene LDPE sheet, both pristine and surface treated. Some samples have been treated by air plasma at low vacuum pressures for different periods of time; others have been washed in ethyl acetate and then plasma treated before the resistivity measurement. The sets of resistivity measurements form the various treatments are compared below. X-ray photoelectron spectroscopy (XPS) has also been used to investigate and account for the observed variations in surface resistivity

  15. Measurement of Dynamic Resistance in Resistance Spot Welding

    DEFF Research Database (Denmark)

    Wu, Pei; Zhang, Wenqi; Bay, Niels

    Through years, the dynamic resistance across the electrodes has been used for weld quality estimation and contact resistance measurement. However, the previous methods of determining the dynamic resistance were mostly based on measuring the voltage and current on the secondary side...... of the transformer in resistance welding machines, implying defects from induction noise and interference with the leads connected to the electrodes for measuring the voltage. In this study, the dynamic resistance is determined by measuring the voltage on the primary side and the current on the secondary side......, as another application, the proposed method is used to measure the faying surface contact resistance....

  16. Rolling Resistance Measurement and Model Development

    DEFF Research Database (Denmark)

    Andersen, Lasse Grinderslev; Larsen, Jesper; Fraser, Elsje Sophia

    2015-01-01

    There is an increased focus worldwide on understanding and modeling rolling resistance because reducing the rolling resistance by just a few percent will lead to substantial energy savings. This paper reviews the state of the art of rolling resistance research, focusing on measuring techniques......, surface and texture modeling, contact models, tire models, and macro-modeling of rolling resistance...

  17. Measurement of Dynamic Resistance in Resistance Spot Welding

    DEFF Research Database (Denmark)

    Wu, Pei; Lu, J.; Zhang, Wenqi

    2007-01-01

    The conventional methods of determining the dynamic resistance were mostly done by measuring the voltage and current at secondary side of transformer in resistance welding machines, in which the measuring set-up normally interferes with the movement of electrode, and the measuring precision is in...

  18. Measurement of Dynamic Resistance in Resistance Spot Welding

    DEFF Research Database (Denmark)

    Wu, Pei; Lu, J.; Zhang, Wenqi

    2007-01-01

    The conventional methods of determining the dynamic resistance were mostly done by measuring the voltage and current at secondary side of transformer in resistance welding machines, in which the measuring set-up normally interferes with the movement of electrode, and the measuring precision...... is influenced by inductive noise caused by the high welding current. In this study, the dynamic resistance is determined by measuring the voltage at primary side and current at secondary side. This increases the accuracy of measurement because of higher signal-noise ratio, and allows to apply to in...

  19. Wastewater Sampling Methodologies and Flow Measurement Techniques.

    Science.gov (United States)

    Harris, Daniel J.; Keffer, William J.

    This document provides a ready source of information about water/wastewater sampling activities using various commercial sampling and flow measurement devices. The report consolidates the findings and summarizes the activities, experiences, sampling methods, and field measurement techniques conducted by the Environmental Protection Agency (EPA),…

  20. Direct measurement of the resistivity weighting function

    Science.gov (United States)

    Koon, D. W.; Chan, Winston K.

    1998-12-01

    We have directly measured the resistivity weighting function—the sensitivity of a four-wire resistance measurement to local variations in resistivity—for a square specimen of photoconducting material. This was achieved by optically perturbing the local resistivity of the specimen while measuring the effect of this perturbation on its four-wire resistance. The weighting function we measure for a square geometry with electrical leads at its corners agrees well with calculated results, displaying two symmetric regions of negative weighting which disappear when van der Pauw averaging is performed.

  1. Using data on resistance prevalence per sample in the surveillance of antimicrobial resistance

    DEFF Research Database (Denmark)

    Vieira, Antonio; Shuyu, Wu; Jensen, Lars Bogø

    2008-01-01

    quantitative data on antimicrobial resistance (resistance prevalence per sample). Methods: In this study, a total of 98 faecal samples from slaughter pigs were tested for tetracycline and sulphonamide resistance in Escherichia coli using the single colony method, and these results were compared......Objectives: In most existing antimicrobial resistance monitoring programmes, one single bacterial colony from each collected sample is susceptibility tested against a panel of antimicrobials. Detecting the proportion of colonies resistant to different antimicrobials in each sample can provide...... and occurrence of resistance, there is a need to move towards a more quantitative approach when dealing with antimicrobial resistance in a population, and the resistance prevalence per sample method can provide some of this additional information....

  2. Comparing pulmonary resistance measured with an esophageal balloon to resistance measurements with an airflow perturbation device

    International Nuclear Information System (INIS)

    Coursey, D C; Johnson, A T; Scharf, S M

    2010-01-01

    The airflow perturbation device (APD) perturbs flow and mouth pressure during regular breathing. Ratios of mouth pressure perturbation magnitudes to flow perturbation magnitudes were used to calculate inspiratory, expiratory and average respiratory resistances. Resistance measurements with the APD were compared to pulmonary resistances directly measured with an esophageal balloon. Six healthy subjects were tested during tidal breathing when known external resistances were added during inspiration, during expiration and during both inspiration and expiration. When the baseline averaged balloon measured pulmonary resistance was subtracted from the baseline averaged APD measured resistance, the difference between them was 0.92 ± 1.25 (mean ± SD) cmH 2 O L –1 s –1 . Compared to the magnitude of the known increase in the added resistance, the APD measured resistance increased by 79%, whereas directly measured pulmonary resistance increased only by 56%. During addition of external resistances to both inspiration and expiration, the changes in inspiratory and expiratory pulmonary resistance were only 36% and 62% of the added resistance, respectively. On the other hand, the APD inhalation and exhalation resistance measured between 82% and 76% of the added resistance. We conclude that the APD detects changes in external resistance at least as well as, and probably better than, classical measurements of pulmonary resistance

  3. Gaseous radiocarbon measurements of small samples

    International Nuclear Information System (INIS)

    Ruff, M.; Szidat, S.; Gaeggeler, H.W.; Suter, M.; Synal, H.-A.; Wacker, L.

    2010-01-01

    Radiocarbon dating by means of accelerator mass spectrometry (AMS) is a well-established method for samples containing carbon in the milligram range. However, the measurement of small samples containing less than 50 μg carbon often fails. It is difficult to graphitise these samples and the preparation is prone to contamination. To avoid graphitisation, a solution can be the direct measurement of carbon dioxide. The MICADAS, the smallest accelerator for radiocarbon dating in Zurich, is equipped with a hybrid Cs sputter ion source. It allows the measurement of both, graphite targets and gaseous CO 2 samples, without any rebuilding. This work presents experiences dealing with small samples containing 1-40 μg carbon. 500 unknown samples of different environmental research fields have been measured yet. Most of the samples were measured with the gas ion source. These data are compared with earlier measurements of small graphite samples. The performance of the two different techniques is discussed and main contributions to the blank determined. An analysis of blank and standard data measured within years allowed a quantification of the contamination, which was found to be of the order of 55 ng and 750 ng carbon (50 pMC) for the gaseous and the graphite samples, respectively. For quality control, a number of certified standards were measured using the gas ion source to demonstrate reliability of the data.

  4. Correlation of Scan and Sample Measurements Using Appropriate Sample Size

    International Nuclear Information System (INIS)

    Lux, Jeff

    2008-01-01

    At a former nuclear fuel cycle facility, radiological site characterization performed in the 1990's relied on surface soil samples collected and analyzed on a systematic sampling grid, supplemented by 100% scans of the property to identify soil exceeding decommissioning criteria. Biased sampling was performed as indicated by these measurements to provide more precise delineation of material exceeding the decommissioning criteria of 0.37 Bq/g total thorium (the Criteria). All material exceeding the Criteria was to be excavated for shipment to a licensed disposal facility. The site characterization plan called for collection of ∼100-gram soil samples on a ten-meter grid, at which locations the gamma exposure rate was recorded at a height of 1 meter above grade. While 25-gram aliquots of the soil samples were being counted by gamma spectroscopy, the area was surveyed with a 7.6 cm X 1.27 cm (3'' X 1/2'') NaI gamma detector positioned 1 meter above grade. Count rates were recorded every two seconds while the detector moved at less than one meter per second, resulting in readings at nearly one-meter intervals. Scan records were reviewed; locations exceeding a pre-determined threshold were sampled to identify material exceeding the decommissioning criteria not identified in the systematic sampling effort. Location data (X, Y, and Z) were imported into an interactive database with μR/hr measurements, sample count results, and scan readings. Data was graphically portrayed by color-coding data and superimposing the measurements on maps. Data reviewers could thereby review all types of data superimposed on each other to minimize review time, and to easily identify areas requiring further investigation. Data reviewers observed that in one area covering several acres, scan and sample measurements did not correlate. In some locations, scans indicated near-background gamma count rates while soil samples yielded several times the decommissioning criteria. At other locations

  5. The ``cinquefoil" resistive/Hall measurement geometry

    Science.gov (United States)

    Koon, Daniel W.

    2000-03-01

    This talk begins by analyzing the charge transport weighting functions -- the sensitivity of resistive and Hall measurements to local macroscopic inhomogeneities -- of bridge-shaped transport specimens. As expected, such measurements sample only that region of the specimen between the central voltage electrodes, in the limit of narrow current channels connected by even narrower arms to the voltage electrodes. The bridge geometry has a few advantages over the van der Pauw cloverleaf geometry -- including ease in zeroing out the null-field Hall voltage -- but also some disadvantages. The talk concludes with an analysis of a hybrid geometry, the “cinquefoil” or five-leafed clover, which combines the best features of both.

  6. Measurement of neutron activation in concrete samples

    International Nuclear Information System (INIS)

    Zagar, T.; Ravnik, M.

    2000-01-01

    The results of activation studies of ordinary and barytes concrete samples relevant for research reactor decommissioning are given. Five important long-lived radioactive isotopes ( 54 Mn, 60 Co, 65 Zn, 133 Ba, and 152 Eu) were identified from the gamma-ray spectra measured in the irradiated concrete samples. Activation of these samples was also calculated using ORIGEN2 code. Comparison of calculated and measured results is given. (author)

  7. Robust weak measurements on finite samples

    International Nuclear Information System (INIS)

    Tollaksen, Jeff

    2007-01-01

    A new weak measurement procedure is introduced for finite samples which yields accurate weak values that are outside the range of eigenvalues and which do not require an exponentially rare ensemble. This procedure provides a unique advantage in the amplification of small nonrandom signals by minimizing uncertainties in determining the weak value and by minimizing sample size. This procedure can also extend the strength of the coupling between the system and measuring device to a new regime

  8. Witness sample preparation for measuring antireflection coatings.

    Science.gov (United States)

    Willey, Ronald R

    2014-02-01

    Measurement of antireflection coating of witness samples from across the worldwide industry has been shown to have excess variability from a sampling taken for the OSA Topical Meeting on Optical Interference Coatings: Measurement Problem. Various sample preparation techniques have been discussed with their limitations, and a preferred technique is recommended with its justification, calibration procedures, and limitations. The common practice of grinding the second side to reduce its reflection is less than satisfactory. One recommended practice is to paint the polished second side, which reduces its reflection to almost zero. A method to evaluate the suitability of given paints is also described.

  9. Calculation of skid resistance from texture measurements

    Directory of Open Access Journals (Sweden)

    Andreas Ueckermann

    2015-02-01

    Full Text Available There is a wide range of routine skid resistance measurement devices on the market. All of them are measuring the friction force between a rubber wheel and the wetted road surface. Common to all of them is that they are relatively complex and costly because generally a truck carrying a large water tank is needed to wet the surface with a defined water layer. Because of the limited amount of water they can carry they are limited in range. Besides that the measurement is depending on factors like water film thickness, temperature, measurement speed, rubber aging, rubber wear and even road evenness and curviness. All of these factors will affect the skid resistance and are difficult to control. We present a concept of contactless skid resistance measurement which is based on optical texture measurement and consists of two components: measurement of the pavement texture by means of an optical measuring system and calculation of the skid resistance based on the measured texture by means of a rubber friction model. The basic assumptions underlying the theoretical approach and the model itself based on the theory of Persson are presented. The concept is applied to a laboratory device called Wehner/Schulze (W/S machine to prove the theoretical approach. The results are very promising. A strong indication could be provided that skid resistance could be measured without contact in the future.

  10. Sampling Criterion for EMC Near Field Measurements

    DEFF Research Database (Denmark)

    Franek, Ondrej; Sørensen, Morten; Ebert, Hans

    2012-01-01

    An alternative, quasi-empirical sampling criterion for EMC near field measurements intended for close coupling investigations is proposed. The criterion is based on maximum error caused by sub-optimal sampling of near fields in the vicinity of an elementary dipole, which is suggested as a worst......-case representative of a signal trace on a typical printed circuit board. It has been found that the sampling density derived in this way is in fact very similar to that given by the antenna near field sampling theorem, if an error less than 1 dB is required. The principal advantage of the proposed formulation is its...

  11. Sensitivity of surface resistance measurement of HTS thin films by ...

    Indian Academy of Sciences (India)

    field distribution in the resonators. The microwave surface resistance of the superconducting sample is then extracted from the measured Q value as a function of temperature. The sensitivity of the Rs measurement, that is, the relative change in the Q value with the change in the Rs value is determined for each resonator.

  12. Vancomycin-resistant enterococci (VRE) in meat and environmental samples.

    Science.gov (United States)

    Messi, Patrizia; Guerrieri, Elisa; de Niederhäusern, Simona; Sabia, Carla; Bondi, Moreno

    2006-03-15

    We investigated the spread of vancomycin-resistant enterococci (VRE) in strains from meat and environmental samples and the location of glycopeptide-resistance determinants in VanA isolates. VRE and VSE (vancomycin-sensitive enterococci) resistance patterns to six antimicrobials were also evaluated. A total of 59 meat isolates (35%) and 119 environmental isolates (26.5%) were glycopeptide resistant enterococci. In particular, 10.7% meat isolates belonged to the VanA, 8.3% to VanB and 16% to VanC phenotypes. Environmental samples presented 0.7% VanA, 14.5% VanB, and 11.4% VanC strains. Evident differences were not observed among the resistance patterns of VRE and VSE isolates. Neither an important difference was observed comparing the resistance patterns in enterococci from meat and environment. In particular a low incidence of beta-lactamic resistant strains was found, whereas high rates of resistance were observed for streptomycin (85.7% and 92.8%), kanamycin (79.7% and 96%) and gentamycin (85.1% and 91.7%). An intermediate rate of resistant bacteria emerged for erythromycin (35.1% and 10.5%). All VanA isolates independent of origin had more plasmids with different molecular weights. PCR amplification of the 732 bp fragment in plasmids from the VanA strains confirmed affiliation to the vanA gene cluster and the extrachromosomal location of the glycopeptide-resistance determinants. Our study suggests that food and environment play a potential role as reservoirs of resistance determinants, prompting the need to undertake epidemiological and molecular studies to evaluate the mobility of these genes.

  13. Radon measurement in Malaysia water samples

    International Nuclear Information System (INIS)

    Ibrahim, A.B.; Rosli Mahat; Yusof Md Amin

    1995-01-01

    This paper reported the results of the measurement of radon in local water. The water samples collected were rainwater, river water, seawater, well water or ground water at area of State of Selangor and Kuala Lumpur. The samples were collected in scintillation cell ZnS(Ag) through Radon Degassing Unit RDU 200. Alpha activity was counted with scintillation counters RD 200 at energy 5.5 MeV. (author)

  14. Antibiotic resistant bacteria in faecal samples of apparently healthy ...

    African Journals Online (AJOL)

    The occurrence of antibiotic-resistant bacteria in faeces of apparently healthy individual volunteers was investigated. Faecal samples were collected from 216 individuals comprising 138 adults (70 males and 68 females) and 78 children aged between 4 months and 42 years (mean age was 30.2 months). Individuals on ...

  15. Measurements of plutonium in environmental samples

    International Nuclear Information System (INIS)

    D'Alberti, F.; Risposi, L.

    1996-01-01

    Within the activities connected with the start up of the PETRA Laboratory (Processo per l'Estrazione di Terre Rare ed Attinidi, i.e. process for extraction of rare earths and actinides), the Radiation Protection Unit of the J.R.C.-Ispra has carried out a well planned set of experimental measurements aimed at evaluating the zero point of the isotopes of plutonium in environmental samples by alfa spectrometry. After the International Moratorium in 1963, no release of plutonium has occurred in the environment apart from the burn up of SNAP 9A satellite in April 1964. Since then the plutonium concentration in air and in fallout samples has been continuously decreasing requiring, therefore, optimization of both instrumentation and experimental measurement procedures in order to obtain better sensibilities. In this work, the experimental methodology followed at the J.R.C.-Ispra for measurements of plutonium concentration in air, deposition and soil is described and the plutonium behaviour in these samples is reported and discussed starting from 1961

  16. Measuring Learning Resistance to Workplace Training

    Science.gov (United States)

    Taylor, Jonathan E.; Lounsbury, John

    2016-01-01

    Training Transfer has been a topic bearing considerable mention over the past several decades. This article focuses on the connection between training transfer and learning resistance and presents research findings describing the design, creation, and testing of the Learning Efficiency Inventory (LEI). The LEI was designed to measure learning…

  17. Method of separate determination of high-ohmic sample resistance and contact resistance

    Directory of Open Access Journals (Sweden)

    Vadim A. Golubiatnikov

    2015-09-01

    Full Text Available A method of separate determination of two-pole sample volume resistance and contact resistance is suggested. The method is applicable to high-ohmic semiconductor samples: semi-insulating gallium arsenide, detector cadmium-zinc telluride (CZT, etc. The method is based on near-contact region illumination by monochromatic radiation of variable intensity from light emitting diodes with quantum energies exceeding the band gap of the material. It is necessary to obtain sample photo-current dependence upon light emitting diode current and to find the linear portion of this dependence. Extrapolation of this linear portion to the Y-axis gives the cut-off current. As the bias voltage is known, it is easy to calculate sample volume resistance. Then, using dark current value, one can determine the total contact resistance. The method was tested for n-type semi-insulating GaAs. The contact resistance value was shown to be approximately equal to the sample volume resistance. Thus, the influence of contacts must be taken into account when electrophysical data are analyzed.

  18. The Rate of Inducible MLSB Resistance in the Methicillin-Resistant Staphylococci Isolated From Clinical Samples.

    Science.gov (United States)

    Toka Özer, Türkan

    2016-09-01

    Staphylococci are one of the most common pathogens in nasocomial and community-acquired infections. Methicillin-resistant staphylococci are known to be resistant against all beta-lactam antibiotics. Therefore, non-beta-lactam antibiotics such as macrolide and lincosamides can be used. Resistance to those antibiotics may lead to therapeutic failure. The purpose of the present study was to determine the prevalence of macrolide-lincosamide-streptogramin B (MLSB ) resistance by using D-test in staphylococcal isolates from various clinical samples. Seventy-one methicillin-resistant Staphylococcus isolates (six S. aureus, 65 coagulase negative staphylococci) were included in this study. Staphylococci were identified with conventional methods. According to Clinical Laboratory Standards Institute (CLSI) criteria, susceptibility testing was performed by Kirby-Bauer disk diffusion method. One of six (16.6%) methicillin-resistant S. aureus isolates and 19 of 65 (29.2%) methicillin-resistant coagulase-negative staphylococci (MR-CNS) were detected as D-test positive. Twenty of 71 (28.1%) staphylococcal isolates detected as D-test positive. Inducible clindamycin resistance was found at a higher rate in MR-CNS. Since the resistant community and hospital acquired staphylococcal infections have become a therapeutic problem, it is very important to detect MLSB resistance routinely in microbiology laboratories. D-test is a cheap and reliable diagnostic method that can be performed in every laboratory. © 2015 Wiley Periodicals, Inc.

  19. How to measure hepatic insulin resistance?

    Science.gov (United States)

    Choukem, S-P; Gautier, J-F

    2008-12-01

    The liver plays a pivotal role in energy metabolism. Under the control of hormones, especially insulin, the liver stores or releases glucose as needed by the body's systems. It is also responsible for an important part of non-esterified fatty-acid and aminoacid metabolism. Assessing hepatic insulin resistance is almost always synonymous with measuring hepatic glucose production (HGP) and calculating indices of hepatic insulin resistance. The most frequently used method to this end is the isotope dilution technique using a tracer. Among tracers, stable isotope-labelled glucose molecules are particularly advantageous over radioactive isotope-labelled glucose and are, therefore, the tracers of choice. The tracer is infused either on its own after an overnight fast to evaluate fasting HGP, or with some among the usual insulin-sensitivity tests to assess HGP suppression by insulin and/or glucose. In a fasting state, HGP is easily calculated whereas, during insulin or glucose infusion, some formula are needed to correct for the non-steady-state condition. The hepatic insulin-resistance index is the product of HGP and the corresponding plasma insulin concentration. Although subject to error, the isotope dilution method nevertheless remains an irreplaceable tool for assessing hepatic insulin resistance in clinical research. From a practical point of view, some easily obtainable indices and clinical or biochemical parameters can serve as surrogates or markers of hepatic insulin resistance in clinical practice. Finally, drugs such as metformin or glitazones can improve hepatic insulin resistance, hence their use in hepatic insulin-resistant states such as type 2 diabetes and non-alcoholic fatty liver disease.

  20. A new measurement method for the dynamic resistance signal during the resistance spot welding process

    International Nuclear Information System (INIS)

    Wang, Lijing; Hou, Yanyan; Zhao, Jian; Zhang, Hongjie; Xi, Tao; Qi, Xiangyang; Li, Yafeng

    2016-01-01

    To measure the dynamic resistance signal during the resistance spot welding process, some original work was carried out and a new measurement method was developed. Compared with the traditional method, using the instantaneous electrode voltage and welding current at peak current point in each half cycle, the resistance curve from the newly proposed method can provide more details of the dynamic resistance changes over time. To test the specific performance of the proposed method, a series of welding experiments were carried out and the tensile shear strengths of the weld samples were measured. Then, the measurement error of the proposed method was evaluated. Several features were extracted from the dynamic resistance curves. The correlations between the extracted features and weld strength were analyzed and the results show that these features are closely related to the weld strength and they can be used for welding quality monitoring. Moreover, the dynamic resistance curve from the newly proposed method can also be used to monitor some abnormal welding conditions. (paper)

  1. Resistance behaviour of inducible clindamycin-resistant staphylococci from clinical samples and aquatic environments.

    Science.gov (United States)

    Heß, Stefanie; Gallert, Claudia

    2014-11-01

    In this study, the species diversity of staphylococci with inducible resistance to macrolides, lincosamides and streptogramin B (MLSB) isolated from clinical samples, sewage and river water was investigated. Inducible clindamycin resistance was tested using a D-test and macrodilution assays. Inducible cross-resistance (iMLSB phenotype) was examined by PCR of erm gene classes A, B, C, F, G, Q, T and 43. Although ermC was the most frequently detected resistance gene in iMLSB phenotypes of environmental staphylococci (61.2%), resistance genes encoding iMLSB were more diverse than in staphylococci from hospital samples. In 22.4% of iMLSB staphylococci from aquatic environments, none of the eight tested erm genes was found. Those isolates and erm43-expressing Staphylococcus lentus displayed low erythromycin MICs (3-16 µg ml(-1)) compared with ermC-positive environmental staphylococci (≥256 µg ml(-1)). In contrast to clinical isolates with clearly defined resistance behaviour, resistance patterns against MLSB and MICs for clindamycin of environmental isolates were more diverse. Although the abundance of iMLSB staphylococci in the aquatic environment was lower than in staphylococci from hospital samples, the diversity of resistance genes encoding this phenotype seemed to be higher. Oleandomycin is the best marker to correlate iMLSB phenotype and the respective erm gene. The phenotypical behaviour of environmental isolates may differ from the resistance pattern of clinical iMLSB staphylococci expressing ermA or ermC, and this should be considered for successful treatment of infections. © 2014 The Authors.

  2. Measurement of curium in marine samples

    Science.gov (United States)

    Schneider, D. L.; Livingston, H. D.

    1984-06-01

    Measurement of environmentally small but detectable amounts of curium requires reliable, accureate, and sensitive analytical methods. The radiochemical separation developed at Woods Hole is briefly reviewed with specific reference to radiochemical interferences in the alpha spectrometric measurement of curium nuclides and to the relative amounts of interferences expected in different oceanic regimes and sample types. Detection limits for 242 Cm and 244 Cm are ultimately limited by their presence in the 243Am used as curium yield monitor. Environmental standard reference materials are evaluated with regard to curium. The marine literature is reviewed and curium measurements are discussed in relation to their source of introduction to the environment. Sources include ocean dumping of low-level radioactive wastes and discharges from nuclear fuel reporcessing activities, In particular, the question of a detectable presence of 244Cm in global fallout from nuclear weapons testing is addressed and shown to be essentially negligible. Analyses of Scottish coastal sedimantes show traces of 242Cm and 244Cm activity which are believed to originate from transport from sources in the Irish Sea.

  3. A simplified model to estimate thermal resistance between carbon nanotube and sample in scanning thermal microscopy

    Science.gov (United States)

    Nazarenko, Maxim; Rosamond, Mark C.; Gallant, Andrew J.; Kolosov, Oleg V.; Dubrovskii, Vladimir G.; Zeze, Dagou A.

    2017-12-01

    Scanning thermal microscopy (SThM) is an attractive technique for nanoscale thermal measurements. Multiwalled carbon nanotubes (MWCNT) can be used to enhance a SThM probe in order to drastically increase spatial resolution while keeping required thermal sensitivity. However, an accurate prediction of the thermal resistance at the interface between the MWCNT-enhanced probe tip and a sample under study is essential for the accurate interpretation of experimental measurements. Unfortunately, there is very little literature on Kapitza interfacial resistance involving carbon nanotubes under SThM configuration. We propose a model for heat conductance through an interface between the MWCNT tip and the sample, which estimates the thermal resistance based on phonon and geometrical properties of the MWCNT and the sample, without neglecting the diamond-like carbon layer covering the MWCNT tip. The model considers acoustic phonons as the main heat carriers and account for their scattering at the interface based on a fundamental quantum mechanical approach. The predicted value of the thermal resistance is then compared with experimental data available in the literature. Theoretical predictions and experimental results are found to be of the same order of magnitude, suggesting a simplified, yet realistic model to approximate thermal resistance between carbon nanotube and sample in SThM, albeit low temperature measurements are needed to achieve a better match between theory and experiment. As a result, several possible avenues are outlined to achieve more accurate predictions and to generalize the model.

  4. Loss-resistant unambiguous phase measurement

    OpenAIRE

    Dinani, Hossein T.; Berry, Dominic W.

    2014-01-01

    Entangled multi-photon states have the potential to provide improved measurement accuracy, but are sensitive to photon loss. It is possible to calculate ideal loss-resistant states that maximize the Fisher information, but it is unclear how these could be experimentally generated. Here we propose a set of states that can be obtained by processing the output from parametric down-conversion. Although these states are not optimal, they provide performance very close to that of optimal states for...

  5. Measurement of Difficult-to-Measure Radionuclides in Environmental Samples

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Mun Ja; Choi, G S; Cho, Y. H.; Jeong, K. H.; Kim, H. R.; Park, D. W.

    2010-07-15

    Analytical procedures for technetium-99 and iodine-129 radioactivity in groundwater and seaweed sampled around radioactive waste disposal site were established. Quality control procedure for the radiochemical analysis was also prepared. Radiochemical analysis for technetium-99 in 72 groundwaters sampled around disposal site from 2007 to 2009 was performed. Radioactivities of technetium-99 were below MDA. The MDAs were obtained from <0.0643 to <0.0837 Bq/L. Radiochemical analysis for iodine-129 in 72 groundwaters was also carried out. Radioactivities of iodine-129 were below MDA. The MDAs were obtained from <0.0175 to <0.0470 Bq/L. Radioactivity of iodine-129 in 12 seaweeds sampled around disposal site was measured. Radioactivity of iodine-129 in the seaweeds were below MDA. The MDAs reached from <0.153 to <1.31 Bq/kg-fresh. For the verification of the analytical procedure, our lab. participated inter-comparison performance test organized by NPL. From the result of data evaluation by NPL, our lab obtained 'In Agreement' in the analysis of technetium-99 and iodine-129 of water sample

  6. Diffusivity and electrical resistivity measurements in rock matrix around fractures

    International Nuclear Information System (INIS)

    Kumpulainen, H.; Uusheimo, K.

    1989-12-01

    Microfracturing of rock matrix around permeable fractures was studied experimentally from drill core samples around major fractures. The methods used were diffusion measurements using a 36 Cl-tracer and electrical resistivity measurements. Rock samples were from the Romuvaara investigation site, the granite specimen around a partially filled carbonate fracture (KR4/333 m) and gneiss specimen around a slickenside fracture (KR1/645 m). A consistent difference of one to two orders of magnitude in the levels of the methods with regard to the effective diffusion coefficients for Cl - -ion was found, the electrical resistivity measurement giving higher values. On the basis of the diffusion measurements the diffusion porosities could be calculated but these remained one to two orders of magnitude lower than that expected for granitic rocks using the water saturation method. A possible reason for these differences could have been the low, in some cases 0.004 M NaC1-concentration in the diffusion experiments vs. the 1 M NaCl-concentration used in the electrical resistivity measurements. Due to the small number of specimens and cross sectional areas of only 2 cm 2 , rock inhomogeneity effects were significant making the interpretation of the results somewhat troublesome. Porosities on fracture surfaces seemed to be higher than in the deeper, more intact rock matrix

  7. Electrical resistance behavior with gamma radiation dose in bulk carbon nanostrutured samples

    International Nuclear Information System (INIS)

    Lage, J.; Leyva, A.; Pinnera, I.; Desdin, L. F.; Abreu, Y.; Cruz, C. M.; Leyva, D.; Toledo, C.

    2013-01-01

    The aim of this paper is to study the effects of 60 Co gamma radiation on the electrical resistance and V-I characteristic of bulk carbon nano structured samples obtained by electric arc discharge in water method. Images of pristine samples obtained with scanning electron, and the results in graphical form of the electrical characterization of irradiated samples are presented in the text. It was observed that the electrical resistance vs. dose behavior shows an initial increment reaching the maximum at approximately 135 kGy, followed by a drop of the resistance values. These behaviors are associated with the progressive generation of radiation induced defects in the sample, whose number increases to reach saturation at 135 kGy. From this dose, defects could lead to cross-links between different nano structures present in the sample conducting to a gradually drop in electrical resistance. The measured V-I curves show that, increasing exposure to the 60 Co gamma radiation, the electrical properties of the studied samples transit from a semiconductor towards a predominantly metallic behavior. These results were compared with those obtained for a sample of graphite powder irradiated under the same conditions. (Author)

  8. Sensitivity study of micro four-point probe measurements on small samples

    DEFF Research Database (Denmark)

    Wang, Fei; Petersen, Dirch Hjorth; Hansen, Torben Mikael

    2010-01-01

    The authors calculate the sensitivities of micro four-point probe sheet resistance and Hall effect measurements to the local transport properties of nonuniform material samples. With in-line four-point probes, the measured dual configuration sheet resistance is more sensitive near the inner two...... probes than near the outer ones. The sensitive area is defined for infinite film, circular, square, and rectangular test pads, and convergent sensitivities are observed for small samples. The simulations show that the Hall sheet resistance RH in micro Hall measurements with position error suppression...... is sensitive to both local carrier density and local carrier mobility because the position calculation is affected in the two pseudo-sheet-resistance measurements needed for the position error suppression. Furthermore, they have also simulated the sensitivity for the resistance difference Delta...

  9. The realization of temperature controller for small resistance measurement system

    Science.gov (United States)

    Sobecki, Jakub; Walendziuk, Wojciech; Idzkowski, Adam

    2017-08-01

    This paper concerns the issues of construction and experimental tests of a temperature stabilization system for small resistance increments measurement circuits. After switching the system on, a PCB board heats up and the long-term temperature drift altered the measurement result. The aim of this work is reducing the time of achieving constant nominal temperature by the measurement system, which would enable decreasing the time of measurements in the steady state. Moreover, the influence of temperatures higher than the nominal on the measurement results and the obtained heating curve were tested. During the working process, the circuit heats up to about 32 °C spontaneously, and it has the time to reach steady state of about 1200 s. Implementing a USART terminal on the PC and an NI USB-6341 data acquisition card makes recording the data (concerning temperature and resistance) in the digital form and its further processing easier. It also enables changing the quantity of the regulator settings. This paper presents sample results of measurements for several temperature values and the characteristics of the temperature and resistance changes in time as well as their comparison with the output values. The object identification is accomplished due to the Ziegler-Nichols method. The algorithm of determining the step characteristics parameters and examples of computations of the regulator settings are included together with example characteristics of the object regulation.

  10. Apparatus for measuring resistance change only in a cell analyzer and method for calibrating it

    Science.gov (United States)

    Hoffman, Robert A.

    1980-01-01

    The disclosure relates to resistance only monitoring and calibration in an electrical cell analyzer. Sample and sheath fluid flows of different salinities are utilized, the sample flow being diameter modulated to produce a selected pattern which is compared to the resistance measured across the flows.

  11. A new surface resistance measurement method with ultrahigh sensitivity

    International Nuclear Information System (INIS)

    Liang, Changnian.

    1993-01-01

    A superconducting niobium triaxial cavity has been designed and fabricated to study residual surface resistance of planar superconducting materials. The edge of a 25.4 mm or larger diameter sample in the triaxial cavity is located outside the strong field region. Therefore, the edge effects and possible losses between the thin film and the substrate have been minimized, ensuring that induced RF losses are intrinsic to the test material. The fundamental resonant frequency of the cavity is the same as the working frequency of CEBAF cavities. The cavity has a compact size compared to its TE 011 counterpart, which makes it more sensitive to the sample's loss. For even higher sensitivity, a calorimetry method has been used to measure the RF losses on the superconducting sample. At 2 K, a 2 μK temperature change can be resolved by using carbon resistor sensors. The temperature distribution caused by RF heating is measured by 16 carbon composition resistor sensors. A 0.05 μW heating power can be detected as such a resolution, which translates to a surface resistance of 0.02 nΩ at a surface magnetic field of 52 Oe. This is the most sensitive device for surface resistance measurements to date. In addition, losses due to the indium seal, coupling probes, field emission sites other than the sample, and all of the high field resonator surface, are excluded in the measurement. Surface resistance of both niobium and high-Tc superconducting thin films has been measured. A low R s of 35.2 μΩ was measured for a 25.4 mm diameter YBa 2 Cu 3 O 7 thin film at 1.5 GHz and at 2 K. The measurement result is the first result for a large area epitaxially grown thin film sample at such a low RF frequency. The abrupt disappearance of multipacting between two parallel plates has been observed and monitored with the 16 temperature mapping sensors. Field emission or some field dependent anomalous RF losses on the niobium plate have also been observed

  12. 47 CFR 73.54 - Antenna resistance and reactance measurements.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Antenna resistance and reactance measurements... SERVICES RADIO BROADCAST SERVICES AM Broadcast Stations § 73.54 Antenna resistance and reactance measurements. (a) The resistance of an omnidirectional series fed antenna is measured at either the base of the...

  13. Measurement of carbon-14 in hydrological samples

    International Nuclear Information System (INIS)

    Hussain, S.D.

    1991-11-01

    Thermal neutrons produced by cosmic rays or nuclear weapon tests interact with atmospheric nitrogen resulting in the formation of radiocarbon which, after oxidation into carbon dioxide, follows the natural carbon cycle. The partial pressure of carbon dioxide in the soil is several times that in the atmosphere due to plant root respiration and decay of organic matter. Water absorbs biogenic carbon dioxide while percolating through the unsaturated zone. The carbon content of groundwater is mainly in the form of bicarbonate ions. The extraction of carbon from water sample as barium carbonate is carried out in the field. Benzene is synthesised from the carbonate sample. The activity of radiocarbon in the synthesised benzene is determined by using a liquid scintillation analyzer. Details of sampling procedure, benzene synthesis, counter calibration and treatment of sample data have been given. 7 figs. (author)

  14. Electrical resistivity measurements to predict abrasion resistance of ...

    Indian Academy of Sciences (India)

    The prediction of Los Angeles (LA) abrasion loss from some indirect tests is useful for practical applications. For this purpose, LA abrasion, electrical resistivity, density and porosity tests were carried out on 27 different rock types. LA abrasion loss values were correlated with electrical resistivity and a good correlation ...

  15. Electrical resistivity measurements to predict abrasion resistance of ...

    Indian Academy of Sciences (India)

    WINTEC

    Abstract. The prediction of Los Angeles (LA) abrasion loss from some indirect tests is useful for practical applications. For this purpose, LA abrasion, electrical resistivity, density and porosity tests were carried out on 27 different rock types. LA abrasion loss values were correlated with electrical resistivity and a good corre-.

  16. Photovoltage versus microprobe sheet resistance measurements on ultrashallow structures

    DEFF Research Database (Denmark)

    Clarysse, T.; Moussa, A.; Parmentier, B.

    2010-01-01

    recently been observed between junction photovoltage (JPV) based tools and a micrometer-resolution four-point probe (M4PP) tool on a variety of difficult, state-of-the-art sub-32-nm complementary metal-oxide semiconductor structures (low energy and cluster implants, with/without halo, flash- and laser......-based millisecond anneal). Conventional four-point probe tools fail on almost all of these samples due to excessive probe penetration, whereas in several cases variable probe spacing (using a conventional spreading resistance probe tool) [T. Clarysse , Mater. Sci. Eng. R. 47, 123 (2004)] still gives useful values...... to within about 20%-35% due to its limited probe penetration (5-10 nm at 5 g load). M4PP measurements give systematically a sensible and reproducible result. This is also the case for JPV-based sheet resistance measurements, although these appear to be prone to correct calibration procedures...

  17. Measurement of radioactivity in water samples

    International Nuclear Information System (INIS)

    Richards, L.

    1990-01-01

    Public concern about the levels of radioactivity release to the environment whether authorised discharges or resulting from nuclear accident, has increased in recent years. Consequently there is increasing pressure for reliable data on the distribution of radioactivity and the extent of its intrusion into food chains and water supplies. As a result a number of laboratories not experienced in radioactivity measurements have acquired nucleonic counting equipment. These notes explore the underlying basics and indicate sources of essential data and information which are required for a better understanding of radioactivity measurements. Particular attention is directed to the screening tests which are usually designated ''gross'' alpha and ''gross'' beta activity measurement. (author)

  18. Cross section measurements on radioactive samples

    Energy Technology Data Exchange (ETDEWEB)

    Koehler, P.E.; O' Brien, H.A.

    1990-01-01

    We have developed a system over the past few years at the Manuel Lujan, Jr. Neutron Scattering Center (LANSCE) for making (n,p) and (n,{alpha}) measurements on (mainly) radioactive nuclei. Measurements have included {sup 7}Be(n,p){sup 7}Li, {sup 14}N(n,p){sup 14}C, {sup 17}O(n,{alpha}){sup 14}C, {sup 22}Na(n,p){sup 22}Ne, {sup 22}Na(n,{alpha}){sup 19}F, {sup 35}Cl(n,p){sup 35}S and {sup 36}Cl(n,p){sup 36}S. The major basic physics motivation for these measurements has been the nuclear astrophysics to be learned. Currently, we are assembling a 4{pi} detector of barium fluoride (BaF{sub 2}) for making (n,{gamma}) measurements on radioactive nuclei with relatively short half lives. Once operational, this new detector should allow us to expand our measurements to many more nuclei, and to a broader range of nuclear physics and nuclear astrophysics issues addressed. Results of recent measurements are given and future plans are discussed. 38 refs., 2 figs.

  19. Measurement of solid flow rates and sampling

    International Nuclear Information System (INIS)

    Caillot, A.

    1984-01-01

    Given the fact that very fine sediments are carriers of numerous pollutant products, in order to be able to ensure realistic and vigilant control of the aquatic environment we have to take into account sedimentary transport. The movement of sediments may be due to natural events (currents, swell, winds), or to human intervention (dredging, emptying of dam reservoirs, release of wastes and so forth). Their circulation, at times highly complex, especially in estuaries, may alternate with periods of rest - and therefore periods of accumulation of pollutants - which may be fairly long. Despite the plethora of available methods and techniques, the amounts of sediment transported by drift or in suspension are very difficult to assess. The physico-chemical nature and the behaviour of these substances in water makes it awkward to select samples, in space and time, for the purpose of analysis. The sampling should be carried out with the mechanical means suited to the circumstances and to the aim in mind. However, by taking into consideration the hydrosedimentary mechanisms known by the hydrologists and sedimentologists it is possible to improve the selection of the sites to be monitored as well as to choose more carefully (and therefore to limit) the samples to be analysed. Environmental monitoring may thus be performed more efficiently and at lower cost. (author)

  20. Resistance among domestic violence offenders: measurement development and initial validation.

    Science.gov (United States)

    Levesque, Deborah A; Velicer, Wayne F; Castle, Patricia H; Greene, R Neil

    2008-02-01

    Batterers' resistance to traditional intervention programs has been well documented. Within a Transtheoretical Model of Change (stage of change) framework, a measure of processes of resistance was developed and administered to 346 adult male domestic violence offenders in treatment. The study yielded a 38-item measure that assesses eight dimensions of resistance: (a) System Blaming, (b) Problems with Partner, (c) Problems with Alliance, (d) Social Justification, (e) Hopelessness, (f) Isolation, (g) Psychological Reactance, and (h) Passive Reactance. The relationship between resistance and stage of change, time in treatment, and partner aggression are reported. Results suggest that we look beyond the most common forms of resistance (e.g., denial and victim-blaming) to identify and address other forms of resistance that may be more internally based and difficult to detect. The processes of resistance measure provides a tool for measuring those types of resistance.

  1. The effect of electrode contact resistance and capacitive coupling on Complex Resistivity measurements

    DEFF Research Database (Denmark)

    Ingeman-Nielsen, Thomas

    2006-01-01

    The effect of electrode contact resistance and capacitive coupling on complex resistivity (CR) measurements is studied in this paper. An equivalent circuit model for the receiver is developed to describe the effects. The model shows that CR measurements are severely affected even at relatively lo...... with the contact resistance artificially increased by resistors. The results emphasize the importance of keeping contact resistance low in CR measurements....

  2. Home built equipment for measuring Hall coefficient and charge carrier concentration, mobility and resistivity

    DEFF Research Database (Denmark)

    Borup, Kasper Andersen; Christensen, Mogens; Blichfeld, Anders Bank

    2011-01-01

    We present here a home built setup for measuring the specific resistivity, hall coefficient, and charge carrier concentration and mobility at elevated temperatures. The system is optimized for measurements of samples ranging between doped semiconductors and high resistivity metals and uses the van...... der Pauw method. The commercial availability of these systems is limited and they are usually not optimized for measurements on samples which show properties characteristic of thermoelectric materials. We give an assessment of the reliability of the measurements and a comparison with a commercial...... system, the Quantum Design, Physical Properties Measurement System (PPMS). Measurements on samples with different resistivity and charge carrier concentration will be shown, covering both ends of the interval of samples we are able to measure as well as the range normally observed for thermoelectrics...

  3. Measurement of the Transverse Spitzer Resistivity during Collisional Magnetic Reconnection

    International Nuclear Information System (INIS)

    Trintchouk, F.; Yamada, M.; Ji, H.; Kulsrud, R.M.; Carter, T.A.

    2000-01-01

    Measurement of the transverse resistivity was carried out in a reconnecting current sheet where the mean free path for the Coulomb collision is smaller than the thickness of the sheet. In a collisional neutral sheet without a guide field, the transverse resistivity is directly related to the reconnection rate. A remarkable agreement is found between the measured resistivity and the classical value derived by L. Spitzer. In his calculation the transverse resistivity for the electrons is higher than the parallel resistivity by a factor of 1.96. The measured values have verified this theory to within 30% errors

  4. Sampling strategies in antimicrobial resistance monitoring: evaluating how precision and sensitivity vary with the number of animals sampled per farm.

    Directory of Open Access Journals (Sweden)

    Takehisa Yamamoto

    Full Text Available Because antimicrobial resistance in food-producing animals is a major public health concern, many countries have implemented antimicrobial monitoring systems at a national level. When designing a sampling scheme for antimicrobial resistance monitoring, it is necessary to consider both cost effectiveness and statistical plausibility. In this study, we examined how sampling scheme precision and sensitivity can vary with the number of animals sampled from each farm, while keeping the overall sample size constant to avoid additional sampling costs. Five sampling strategies were investigated. These employed 1, 2, 3, 4 or 6 animal samples per farm, with a total of 12 animals sampled in each strategy. A total of 1,500 Escherichia coli isolates from 300 fattening pigs on 30 farms were tested for resistance against 12 antimicrobials. The performance of each sampling strategy was evaluated by bootstrap resampling from the observational data. In the bootstrapping procedure, farms, animals, and isolates were selected randomly with replacement, and a total of 10,000 replications were conducted. For each antimicrobial, we observed that the standard deviation and 2.5-97.5 percentile interval of resistance prevalence were smallest in the sampling strategy that employed 1 animal per farm. The proportion of bootstrap samples that included at least 1 isolate with resistance was also evaluated as an indicator of the sensitivity of the sampling strategy to previously unidentified antimicrobial resistance. The proportion was greatest with 1 sample per farm and decreased with larger samples per farm. We concluded that when the total number of samples is pre-specified, the most precise and sensitive sampling strategy involves collecting 1 sample per farm.

  5. Electrical resistivity measurement to predict uniaxial compressive ...

    Indian Academy of Sciences (India)

    and multiple regression analysis. It was seen that the ... The correlation coefficients are generally higher for the multiple regression models than that .... for each regression. A strong linear relation between UCS and resistivity values was found (figure 2). UCS values increase with increasing resistivity values. The equation of ...

  6. electrical resistivity measurements of downscaled homogenous

    African Journals Online (AJOL)

    ES Obe

    Abstract. Knowledge of electrical resistivity for reservoir rocks is crucial for a number of reservoir engineering tasks such as the determination of oil-in-place and the cal- ibration of resistivity logs. Those properties can now be predicted by numerical calculations directly on micro-CT images taken from rock fragments typically ...

  7. Electrical Resistivity Measurements of Downscaled Homogenous ...

    African Journals Online (AJOL)

    Knowledge of electrical resistivity for reservoir rocks is crucial for a number of reservoir engineering tasks such as the determination of oil-in-place and the calibration of resistivity logs. Those properties can now be predicted by numerical calculations directly on micro-CT images taken from rock fragments typically having a ...

  8. Development of a magnetic measurement device for thin ribbon samples

    International Nuclear Information System (INIS)

    Sato, Yuta; Todaka, Takashi; Enokizono, Masato

    2008-01-01

    This paper presents a magnetic measurement device for thin ribbon samples, which are produced by rapid cooling technique. This device enables us to measure magnetic properties easily by only inserting a ribbon sample into a sample holder. The sample holder was made by bakelite to fix any width sample. A long solenoid coil was used to generate a uniform magnetic field and the sample holder was placed at the mid part of the solenoid. The magnetic field strength was measured using a shunt resistor and the magnetic flux density and magnetization in sample ribbons were evaluated by using search coils. The accuracy of measurement was verified with an amorphous metal ribbon sample. Next, we have measured magnetic properties of some magnetic shape memory alloys, which have different compositions. The measured results are compared and we clarified the effect of Sm contents on the magnetic properties

  9. Plethysmographic measurements of specific airway resistance in young children

    DEFF Research Database (Denmark)

    Bisgaard, Hans; Nielsen, Kim G

    2005-01-01

    Validated methods for lung function measurements in young children are lacking. Plethysmographic measurement of specific airway resistance (sRaw) provides such a method applicable from 2 years of age. sRaw gauges airway resistance from the measurements of the pressure changes driving the airflow ...

  10. Plethysmographic measurements of specific airway resistance in young children

    DEFF Research Database (Denmark)

    Bisgaard, Hans; Nielsen, Kim G

    2005-01-01

    Validated methods for lung function measurements in young children are lacking. Plethysmographic measurement of specific airway resistance (sRaw) provides such a method applicable from 2 years of age. sRaw gauges airway resistance from the measurements of the pressure changes driving the airflow...

  11. Awake measures of nasal resistance and upper airway resistance on CPAP during sleep.

    Science.gov (United States)

    Masdeu, Maria J; Seelall, Vijay; Patel, Amit V; Ayappa, Indu; Rapoport, David M

    2011-02-15

    Since on CPAP, the nose is the primary determinant of upper airway resistance, we assess utility of noninvasive measures of nasal resistance during wakefulness as a predictor of directly assessed upper airway resistance on CPAP during sleep in patients with obstructive sleep apnea/hypopnea syndrome. Patients with complaints of snoring and excessive daytime sleepiness were recruited. 14 subjects underwent daytime evaluations including clinical assessment, subjective questionnaires to assess nasal symptoms and evaluation of nasal resistance with acoustic rhinometry (AR) and active anterior rhinomanometry (RM) in the sitting and supine positions. Patients underwent nocturnal polysomnography on optimal CPAP with measurements of supraglottic pressure to evaluate upper airway resistance. Comparisons were made between nasal resistance using AR and RM during wakefulness, and between AR and RM awake and upper airway resistance during sleep. Our study shows that measures of awake nasal resistance using AR and RM had little or no correlation to each other in the sitting position, whereas there was significant but weak correlation in the supine position. Upper airway resistance measured while on CPAP during sleep did not show significant relationships to any of the awake measures of nasal resistance (AR or RM). Awake measurements of nasal resistance do not seem to be predictive of upper airway resistance during sleep on CPAP.

  12. Effects of macroscopic inhomogeneities on resistive and Hall measurements on crosses, cloverleafs, and bars

    Science.gov (United States)

    Koon, D. W.; Knickerbocker, C. J.

    1996-12-01

    The effect of macroscopic inhomogeneities on resistivity and Hall angle measurements is studied by calculating weighting functions (the relative effect of perturbations in a local transport property on the measured global average for the object) for cross, cloverleaf, and bar-shaped geometries. The ``sweet spot,'' the region in the center of the object that the measurement effectively samples, is smaller for crosses and cloverleafs than for the circles and squares already studied, and smaller for the cloverleaf than for the corresponding cross. Resistivity measurements for crosses and cloverleafs suffer from singularities and negative weighting, which can be eliminated by averaging two independent resistance measurements, as done in the van der Pauw technique. Resistivity and Hall measurements made on sufficiently narrow bars are shown to effectively sample only the region directly between the voltage probes.

  13. Measurement of trace elements in liver biopsy samples from cattle

    NARCIS (Netherlands)

    Ouweltjes, W.; Zeeuw, de A.C.; Moen, A.; Counotte, G.H.M.

    2007-01-01

    Serum, plasma, or urine samples are usually used for the measurement of the trace elements copper, zinc, iron, selenium, because these samples are easy to obtain; however, these samples are not always appropriate. For example, it is not possible to measure molybdenum, the major antagonist of copper,

  14. Utilization of composite fecal samples for detection of anthelmintic resistance in gastrointestinal nematodes of cattle.

    Science.gov (United States)

    George, Melissa M; Paras, Kelsey L; Howell, Sue B; Kaplan, Ray M

    2017-06-15

    Recent reports indicate that anthelmintic resistance in gastrointestinal nematodes of cattle is becoming increasingly prevalent worldwide. Presently, the fecal egg count reduction test (FECRT) is the only means available for detection of resistance to anthelmintics in cattle herds at the farm level. However, the FECRT is labor and cost intensive, and consequently is only rarely performed on cattle farms unless for research purposes. If costs could be reduced, cattle producers might be more likely to pursue drug resistance testing on their farms. One approach to reducing the cost of the FECRT, is the use of composite fecal samples for performing fecal egg counts (FEC), rather than conducting FEC on fecal samples from 15 to 20 individual animals. In this study FECRT were performed on 14 groups of cattle using both individual and composite FEC methods To measure how well the results of composite sampling reproduce those of individual sampling, Lin's Concordance Correlation Coefficient was utilized to describe both the linear relationship between methods and the slope and y-intercept of the line relating the data sets. There was little difference between the approaches with 98% agreement in mean FEC found between methods Mean FEC based on individual counts ranged between 0 and 670.6 eggs per gram of feces, indicating that the results of this study are applicable to a wide range of FEC levels. Standard error of the mean FEC and range of FEC are reported for each group prior to and following treatment to describe the variability of the data set. There was greater than 95% agreement in drug efficacy between individual and composite sampling methods, demonstrating composite sampling is appropriate to evaluate drug efficacy. Notably, for all groups tested the efficacy calculated by composite sampling was within the 95% confidence interval for efficacy calculated using individual sampling. The use of composite samples was shown to reduce the number of FEC required by 79

  15. Molecular characterization of metallo β-lactamase producing multidrug resistant Pseudomonas aeruginosa from various clinical samples

    Directory of Open Access Journals (Sweden)

    Kalaivani Ramakrishnan

    2014-01-01

    Full Text Available Introduction: Pseudomonas aeruginosa is a potent opportunistic nosocomial human pathogen among Gram-negative bacteria causing various life-threatening infections in patients from Intensive Care Units. This bacterium has become resistant to almost all commonly available antibiotics with limited treatment options. Multi drug resistant P. aeruginosa (MDRPA is a major cause of concern among hospital acquired infections. It uses distinctive resistant mechanisms virtually to all the available antibiotics such as Metallo β-lactamases (MBL production, extended spectrum β-lactamase production (ESBL, up regulation of efflux systems related genes and decreased outer membrane permeability. This study was carried out to find one the predominant resistance mechanisms among MDRPA and the prevalence of corresponding resistance genes. Materials and Methods: MDRPA isolates collected from various clinical samples for a period of 1-year (November 2009-Octo ber 2010 were included to detect the predominant mechanism of resistance using phenotypic and molecular methods. Molecular characterization of all these isolates was done by polymerase chain reaction (PCR for the presence of blaVIM-2, blaIMP-1, blaOXA-23, and blaNDM-1 genes with specific primers. Results: Among 75 MDRPA isolates 84% (63 were MBL producers. Molecular characterization studied by PCR showed the presence of blaVIM-2 gene in 13% of MBL producers. Conclusion: The prevalence of MBLs has been increasing worldwide, particularly among P. aeruginosa, leading to severe limitations in the therapeutic options for the management. Thus, proper resistance screening measures and appropriate antibiotic policy can be strictly adopted by all the healthcare facility providers to overcome these superbugs.

  16. Characterizing Fracture Property Using Resistivity Measured at Different Frequencies

    Energy Technology Data Exchange (ETDEWEB)

    Horne, Roland N. [Stanford Univ., CA (United States); Li, Kewen [Stanford Univ., CA (United States)

    2014-09-30

    The objective was to develop geophysical approaches to detecting and evaluating the fractures created or existing in EGS and other geothermal reservoirs by measuring the resistivity at different frequencies. This project has been divided into two phases: Phase I (first year): Proof of Concept – develop the resistivity approach and verify the effect of frequency on the resistivity in rocks with artificial or natural fractures over a wide range of frequencies. Phase II: Prototyping Part 1 (second year): measure the resistivity in rocks with fractures of different apertures, different length, and different configurations at different frequencies. Part 2 (third year): develop mathematical models and the resistivity method; infer the fracture properties using the measured resistivity data.

  17. Accuracy of micro four-point probe measurements on inhomogeneous samples: A probe spacing dependence study

    DEFF Research Database (Denmark)

    Wang, Fei; Petersen, Dirch Hjorth; Østerberg, Frederik Westergaard

    2009-01-01

    In this paper, we discuss a probe spacing dependence study in order to estimate the accuracy of micro four-point probe measurements on inhomogeneous samples. Based on sensitivity calculations, both sheet resistance and Hall effect measurements are studied for samples (e.g. laser annealed samples...... the probe spacing is smaller than 1/40 of the variation wavelength, micro four-point probes can provide an accurate record of local properties with less than 1% measurement error. All the calculations agree well with previous experimental results.......) with periodic variations of sheet resistance, sheet carrier density, and carrier mobility. With a variation wavelength of ¿, probe spacings from 0.0012 to 1002 have been applied to characterize the local variations. The calculations show that the measurement error is highly dependent on the probe spacing. When...

  18. Considerations for sampling nuclear materials for SNM accounting measurements

    International Nuclear Information System (INIS)

    Brouns, R.J.; Roberts, F.P.; Upson, U.L.

    1978-01-01

    This report presents principles and guidelines for sampling nuclear materials to measure chemical and isotopic content of the material. Development of sampling plans and procedures that maintain the random and systematic errors of sampling within acceptable limits for SNM accounting purposes are emphasized

  19. Thermometry of the system “heat-resistant sample - incident plasma stream”

    Science.gov (United States)

    Sargsyan, M. A.; Chinnov, V. F.; Kavyrshin, D. I.; Gadzhiev, M. Kh; Khromov, M. A.; Chistolinov, A. V.; Senchenko, V. N.

    2017-11-01

    To study the interacting system “heat-resistant sample – an incident plasma stream” a setup of synchronized measurement equipment was developed and tested that recorded the main parameters of such interaction. Heat resistance tests were carried out on the samples of MPG-6 grade isotropic graphite, and samples of pyrolytic graphite that were subjected to a long (60 … 100 s) exposure to nitrogen, argon and air plasma streams at atmospheric pressure. As plasma generators a series of plasma torches with a vortex stabilization of the stream and an expanding anode channels was used. The temperature and composition of the plasma in the jet and near the sample were determined using two AvaSpec2048 and AvaSpec3648 scanning optical spectrometers and the MS5402i spectrograph with the Andor matrix at its outlet. The surface temperature of the sample was determined in real time using three independent ways: two pyrometric systems - a high-speed micro-pyrometer FMP1001 and a two-position visualization of the heated sample by high-speed Motion Pro X3 and VS-FAST cameras, and the spectral analysis of the wide-range thermal radiation of the samples. The main method for determining the rate of material loss during the action of a plasma jet on it was to analyze a two-position synchronous visualization of the “jet-sample” system. When a crater was formed on the surface of the sample under the “dagger” effect of a plasma jet, a video recording system of the crater zone was used, backlit using the “laser knife” method.

  20. Bulk sample self-attenuation correction by transmission measurement

    International Nuclear Information System (INIS)

    Parker, J.L.; Reilly, T.D.

    1976-01-01

    Various methods used in either finding or avoiding the attenuation correction in the passive γ-ray assay of bulk samples are reviewed. Detailed consideration is given to the transmission method, which involves experimental determination of the sample linear attenuation coefficient by measuring the transmission through the sample of a beam of gamma rays from an external source. The method was applied to box- and cylindrically-shaped samples

  1. Measurement of residual stresses in welded sample of dissimilar materials

    International Nuclear Information System (INIS)

    Mansur, Tanius Rodrigues; Gomes, Paulo de Tarso Vida; Scaldaferri, Denis Henrique Bianchi; Martins, Geraldo Antonio Scoralick; Atanazio Filho, Nelson do Nascimento

    2008-01-01

    reactors, what can generate significant residual stresses due so much to the welding procedure as for the difference of the coefficients of thermal expansion of the involved materials. In this work, are shown the results of the measurement of residual tensions in welded sample of steel carbon SA 508 Cl 3 and stainless steel 316L. The Inconel 182 was used as weld metal. (author)

  2. Resistance of GPS samples to non-contact underwater explosion

    Science.gov (United States)

    Dulnev, A. I.; Nekliudova, E. A.

    2017-11-01

    Test data obtained from experiments on GRP test samples in the explosion chamber of Krylov State Research Centre are given. Characteristic features of material damage accumulation and failure processes are discussed based on the experimental data. The sample material damage is assessed versus three indicative effects (criteria): binder failure, fiber rupture, through-thickness penetration (hole). Finite element modelling of the experiments is used for a more detailed analysis of stress-strain state of material samples.

  3. Measuring Resistance to Change at the Within-Session Level

    Science.gov (United States)

    Tonneau, Francois; Rios, Americo; Cabrera, Felipe

    2006-01-01

    Resistance to change is often studied by measuring response rate in various components of a multiple schedule. Response rate in each component is normalized (that is, divided by its baseline level) and then log-transformed. Differential resistance to change is demonstrated if the normalized, log-transformed response rate in one component decreases…

  4. System and method for measuring fluorescence of a sample

    Energy Technology Data Exchange (ETDEWEB)

    Riot, Vincent J

    2015-03-24

    The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.

  5. System and method for measuring fluorescence of a sample

    Energy Technology Data Exchange (ETDEWEB)

    Riot, Vincent J.

    2017-06-27

    The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.

  6. Approximate determination of efficiency for activity measurements of cylindrical samples

    Energy Technology Data Exchange (ETDEWEB)

    Helbig, W. [Nuclear Engineering and Analytics Rossendorf, Inc. (VKTA), Dresden (Germany); Bothe, M. [Nuclear Engineering and Analytics Rossendorf, Inc. (VKTA), Dresden (Germany)

    1997-03-01

    Some calibration samples are necessary with the same geometrical parameters but of different materials, containing known activities A homogeniously distributed. Their densities are measured, their mass absorption coefficients may be unknown. These calibration samples are positioned in the counting geometry, for instance directly on the detector. The efficiency function {epsilon}(E) for each sample is gained by measuring the gamma spectra and evaluating all usable gamma energy peaks. From these {epsilon}(E) the common valid {epsilon}{sub geom}(E) will be deduced. For this purpose the functions {epsilon}{sub mu}(E) for these samples have to be established. (orig.)

  7. Multidrug resistant Salmonellae isolated from blood culture samples ...

    African Journals Online (AJOL)

    This study investigates the prevalence of R-plasmids in Salmonella sp. isolated from blood samples of suspected typhoid patients in Warri, Nigeria. A total of 136 blood samples were collected between May and December,2009 and screened for the presence of Salmonellae using standard blood culture techniques of which ...

  8. Measure of tissue resistivity in experimental electrical burns.

    Science.gov (United States)

    Chilbert, M; Maiman, D; Sances, A; Myklebust, J; Prieto, T E; Swiontek, T; Heckman, M; Pintar, K

    1985-03-01

    Studies were conducted in 14 mongrel dogs to compare resistivities in normal muscle with those from muscle subjected to electrical burns. One-ampere, 60-Hz currents were passed between the hind limbs of the dogs producing injury in three measurement regions of the gracilis muscle. Histology, heart rate, body temperature, arterial and pulmonary artery pressure, cardiac output, hematocrit, leukocyte counts, fibrinogen levels, and platelet levels were determined. Muscle resistivity associated with severe tissue necrosis was 70% lower than control values. Resistivity in tissue showing edema and minimal necrosis decreased 20 to 40% from control values. Muscle showing only edema had a 10 to 30% decrease in resistivity.

  9. A fully automated temperature-dependent resistance measurement setup using van der Pauw method

    Science.gov (United States)

    Pandey, Shivendra Kumar; Manivannan, Anbarasu

    2018-03-01

    The van der Pauw (VDP) method is widely used to identify the resistance of planar homogeneous samples with four contacts placed on its periphery. We have developed a fully automated thin film resistance measurement setup using the VDP method with the capability of precisely measuring a wide range of thin film resistances from few mΩ up to 10 GΩ under controlled temperatures from room-temperature up to 600 °C. The setup utilizes a robust, custom-designed switching network board (SNB) for measuring current-voltage characteristics automatically at four different source-measure configurations based on the VDP method. Moreover, SNB is connected with low noise shielded coaxial cables that reduce the effect of leakage current as well as the capacitance in the circuit thereby enhancing the accuracy of measurement. In order to enable precise and accurate resistance measurement of the sample, wide range of sourcing currents/voltages are pre-determined with the capability of auto-tuning for ˜12 orders of variation in the resistances. Furthermore, the setup has been calibrated with standard samples and also employed to investigate temperature dependent resistance (few Ω-10 GΩ) measurements for various chalcogenide based phase change thin films (Ge2Sb2Te5, Ag5In5Sb60Te30, and In3SbTe2). This setup would be highly helpful for measurement of temperature-dependent resistance of wide range of materials, i.e., metals, semiconductors, and insulators illuminating information about structural change upon temperature as reflected by change in resistances, which are useful for numerous applications.

  10. Antibiotic Resistance in Bacteria Isolated from Commercial Meat Samples and Air Samples Near Agricultural Sites

    OpenAIRE

    Sanchez, Helen Mariette

    2015-01-01

    The rising level of antibiotic resistance is a serious public health issue, posing a global threat to human health. The common practice of applying sub-therapeutic dosages of antibiotics to livestock has been shown to foster the development of antibiotic resistant bacteria (ARB). ARB originating in livestock can reach the general population via multiple pathways: air downwind of animal feeding operations and transportation vehicles, indoor air, soil following land application, surface and gro...

  11. ANTIBIOTIC RESISTANCE IN ENTEROBACTERIACEAE STRAINS ISOLATED FROM CHICKEN AND MILK SAMPLES

    Directory of Open Access Journals (Sweden)

    Lukáš Hleba

    2015-02-01

    Full Text Available Antibiotic resistance and identification of strains in Enterobacteriaceae genera isolated from milk, milk products and rectal swabs of chicken was examined in this experiment. After samples collection cultivation and identification of bacterial strain was done. MALDI TOF MS Biotyper for identification of Enterobacteriaceae strains was used. For susceptibility testing disc diffusion methodology was used according by EUCAST. Results showed high level of ampicillin resistance in isolates from milk and milk samples. The highest streptomycin resistance was detected in isolates from rectal swabs of chicken. After identification, we determined that S. enterica ser. Typhimurium, which was isolated from rectal swabs of chicken showed the most multi-resistance from all identificated strains of Enterobacteriaceae. The most isolates bacterial strain was E. coli, which showed resistance against four antibiotics from rectal swabs of chicken. Also our results showed that the higher resistance level is in rectal swabs of chicken like in milk samples.

  12. Sensitivity of surface resistance measurement of HTS thin films by ...

    Indian Academy of Sciences (India)

    ... field distribution in the resonators. The microwave surface resistance of the superconducting sample is then extracted from the measured value as a function of temperature. The sensitivity of the s measurement, that is, the relative change in the value with the change in the s value is determined for each resonator.

  13. Sensitivity Enhancement of Wheatstone Bridge Circuit for Resistance Measurement

    Directory of Open Access Journals (Sweden)

    Tarikul ISLAM

    2009-08-01

    Full Text Available Present work deals with the development of a low cost, appreciably accurate and sensitive electronic circuit for resistive sensor. The circuit is based on the modification of the Wheatstone bridge using active devices. It helps measurement of the incremental resistance precisely and linearly. It requires few components for its hardware implementation and found to be suitable in case there is small change in resistance due to change in physical quantity or chemical analytes to be measured. Theory of the proposed bridge circuit has been discussed and experimental results have been compared with conventional full bridge circuit. Experiments have been conducted with metallic strain gauge sensor but it can be utilized to other resistive sensors. Results show that the output of the circuit is almost four times more than usual full Wheatstone bridge circuit. Experimental results show that the errors due to the effects of the ambient temperature and connecting lead resistance are minimized.

  14. Resistive Wall Growth Rate Measurements in the Fermilab Recycler

    Energy Technology Data Exchange (ETDEWEB)

    Ainsworth, R. [Fermilab; Adamson, P. [Fermilab; Burov, A. [Fermilab; Kourbanis, I. [Fermilab

    2016-10-05

    Impedance could represent a limitation of running high intensity beams in the Fermilab recycler. With high intensity upgrades foreseen, it is important to quantify the impedance. To do this,studies have been performed measuring the growth rate of presumably the resistive wall instability. The growth rates at varying intensities and chromaticities are shown. The measured growth rates are compared to ones calculated with the resistive wall impedance.

  15. Development of Alternative Rheological Measurements for DWPF Slurry Samples

    International Nuclear Information System (INIS)

    Koopman, D. c.

    2005-01-01

    Rheological measurements are used to evaluate the fluid dynamic behavior of Defense Waste Processing Facility, DWPF, slurry samples. Measurements are currently made on non-radioactive simulant slurries using two state-of-the-art rheometers located at the Aiken County Technical Laboratory, ACTL. Measurements are made on plant samples using a rheometer in the Savannah River National Laboratory, SRNL, Shielded Cells facility. Low activity simulants or plant samples can be analyzed using a rheometer located in a radioactive hood in SRNL. Variations in the rheology of SB2 simulants impacted the interpretation of results obtained in a number of related studies. A separate rheological study was initiated with the following four goals: (1) Document the variations seen in the simulant slurries, both by a review of recent data, and by a search for similar samples for further study. (2) Attempt to explain the variations in rheological behavior, or, failing that, reduce the number of possible causes. In particular, to empirically check for rheometer-related variations. (3) Exploit the additional capabilities of the rheometers by developing new measurement methods to study the simulant rheological properties in new ways. (4) Formalize the rheological measurement process for DWPF-related samples into a series of protocols. This report focuses on the third and fourth goals. The emphasis of this report is on the development and formalization of rheological measurement methods used to characterize DWPF slurry samples. The organization is by rheological measurement method. Progress on the first two goals was documented in a concurrent technical report, Koopman (2005). That report focused on the types and possible causes of unusual rheological behavior in simulant slurry samples. It was organized by the sample being studied. The experimental portion of this study was performed in the period of March to April 2004. A general rheology protocol for routine DWPF slurry samples, Koopman

  16. Development of Alternative Rheological Measurements for DWPF Slurry Samples (U)

    Energy Technology Data Exchange (ETDEWEB)

    Koopman, D. c.

    2005-09-01

    Rheological measurements are used to evaluate the fluid dynamic behavior of Defense Waste Processing Facility, DWPF, slurry samples. Measurements are currently made on non-radioactive simulant slurries using two state-of-the-art rheometers located at the Aiken County Technical Laboratory, ACTL. Measurements are made on plant samples using a rheometer in the Savannah River National Laboratory, SRNL, Shielded Cells facility. Low activity simulants or plant samples can be analyzed using a rheometer located in a radioactive hood in SRNL. Variations in the rheology of SB2 simulants impacted the interpretation of results obtained in a number of related studies. A separate rheological study was initiated with the following four goals: (1) Document the variations seen in the simulant slurries, both by a review of recent data, and by a search for similar samples for further study. (2) Attempt to explain the variations in rheological behavior, or, failing that, reduce the number of possible causes. In particular, to empirically check for rheometer-related variations. (3) Exploit the additional capabilities of the rheometers by developing new measurement methods to study the simulant rheological properties in new ways. (4) Formalize the rheological measurement process for DWPF-related samples into a series of protocols. This report focuses on the third and fourth goals. The emphasis of this report is on the development and formalization of rheological measurement methods used to characterize DWPF slurry samples. The organization is by rheological measurement method. Progress on the first two goals was documented in a concurrent technical report, Koopman (2005). That report focused on the types and possible causes of unusual rheological behavior in simulant slurry samples. It was organized by the sample being studied. The experimental portion of this study was performed in the period of March to April 2004. A general rheology protocol for routine DWPF slurry samples, Koopman

  17. An instrumentation for control and measurement of activated mineral samples

    International Nuclear Information System (INIS)

    Skaarup, P.

    1976-01-01

    A description is given of an instrumentation for control of a pneumatic tube system used to transport mineral samples for activation in a reactor and from there to a detector arrangement. A possible content of uranium in the samples can be seen from the radiation measured. The instrumentation includes a PDP-11 computer and a CAMAC crate

  18. A passive quantitative measurement of airway resistance using depth data.

    Science.gov (United States)

    Ostadabbas, Sarah; Bulach, Christoph; Ku, David N; Anderson, Larry J; Ghovanloo, Maysam

    2014-01-01

    The Respiratory Syncytial Virus (RSV) is the most common cause of serious lower respiratory tract infections in infants and young children. RSV often causes increased airway resistance, clinically detected as wheezing by chest auscultation. In this disease, expiratory flows are significantly reduced due to the high resistance in patient's airway passages. A quantitative method for measuring resistance can have a great benefit to diagnosis and management of children with RSV infections as well as with other lung diseases. Airway resistance is defined as the lung pressure divided by the airflow. In this paper, we propose a method to quantify resistance through a simple, non-contact measurement of chest volume that can act as a surrogate measure of the lung pressure and volumetric airflow. We used depth data collected by a Microsoft Kinect camera for the measurement of the lung volume over time. In our experimentation, breathing through a number of plastic straws induced different airway resistances. For a standard spirometry test, our volume/flow estimation using Kinect showed strong correlation with the flow data collected by a commercially-available spirometer (five subjects, each performing 20 breathing trials, correlation coefficient = 0.88, with 95% confidence interval). As the number of straws decreased, emulating a higher airway obstruction, our algorithm was sufficient to distinguish between several levels of airway resistance.

  19. Intelligent sampling for the measurement of structured surfaces

    International Nuclear Information System (INIS)

    Wang, J; Jiang, X; Blunt, L A; Scott, P J; Leach, R K

    2012-01-01

    Uniform sampling in metrology has known drawbacks such as coherent spectral aliasing and a lack of efficiency in terms of measuring time and data storage. The requirement for intelligent sampling strategies has been outlined over recent years, particularly where the measurement of structured surfaces is concerned. Most of the present research on intelligent sampling has focused on dimensional metrology using coordinate-measuring machines with little reported on the area of surface metrology. In the research reported here, potential intelligent sampling strategies for surface topography measurement of structured surfaces are investigated by using numerical simulation and experimental verification. The methods include the jittered uniform method, low-discrepancy pattern sampling and several adaptive methods which originate from computer graphics, coordinate metrology and previous research by the authors. By combining the use of advanced reconstruction methods and feature-based characterization techniques, the measurement performance of the sampling methods is studied using case studies. The advantages, stability and feasibility of these techniques for practical measurements are discussed. (paper)

  20. Determination and optimization of spatial samples for distributed measurements.

    Energy Technology Data Exchange (ETDEWEB)

    Huo, Xiaoming (Georgia Institute of Technology, Atlanta, GA); Tran, Hy D.; Shilling, Katherine Meghan; Kim, Heeyong (Georgia Institute of Technology, Atlanta, GA)

    2010-10-01

    There are no accepted standards for determining how many measurements to take during part inspection or where to take them, or for assessing confidence in the evaluation of acceptance based on these measurements. The goal of this work was to develop a standard method for determining the number of measurements, together with the spatial distribution of measurements and the associated risks for false acceptance and false rejection. Two paths have been taken to create a standard method for selecting sampling points. A wavelet-based model has been developed to select measurement points and to determine confidence in the measurement after the points are taken. An adaptive sampling strategy has been studied to determine implementation feasibility on commercial measurement equipment. Results using both real and simulated data are presented for each of the paths.

  1. Analytical techniques for measurement of 99Tc in environmental samples

    International Nuclear Information System (INIS)

    Anon.

    1979-01-01

    Three new methods have been developed for measuring 99 Tc in environmental samples. The most sensitive method is isotope dilution mass spectrometry, which allows measurement of about 1 x 10 -12 grams of 99 Tc. Results on analysis of five samples by this method compare very well with values obtained by a second independent method, which involves counting of beta particles from 99 Tc and internal conversion electrons from /sup 97m/Tc. A third method involving electrothermal atomic absorption has also been developed. Although this method is not as sensitive as the first two techniques, the cost per analysis is expected to be considerably less for certain types of samples

  2. Sampling and Control Circuit Board for an Inertial Measurement Unit

    Science.gov (United States)

    Chelmins, David T (Inventor); Powis, Richard T., Jr. (Inventor); Sands, Obed (Inventor)

    2016-01-01

    A circuit board that serves as a control and sampling interface to an inertial measurement unit ("IMU") is provided. The circuit board is also configured to interface with a local oscillator and an external trigger pulse. The circuit board is further configured to receive the external trigger pulse from an external source that time aligns the local oscillator and initiates sampling of the inertial measurement device for data at precise time intervals based on pulses from the local oscillator. The sampled data may be synchronized by the circuit board with other sensors of a navigation system via the trigger pulse.

  3. Friction Coefficient Determination by Electrical Resistance Measurements

    Science.gov (United States)

    Tunyagi, A.; Kandrai, K.; Fülöp, Z.; Kapusi, Z.; Simon, A.

    2018-01-01

    A simple and low-cost, DIY-type, Arduino-driven experiment is presented for the study of friction and measurement of the friction coefficient, using a conductive rubber cord as a force sensor. It is proposed for high-school or college/university-level students. We strongly believe that it is worthwhile planning, designing and performing Arduino…

  4. Measurement of resistance to flow across antroduodenal area during fasting.

    Science.gov (United States)

    Mearin, F; Azpiroz, F; Malagelada, J R

    1986-06-01

    Changes in antroduodenal resistance to flow may participate in the regulation of gastric emptying and duodenogastric reflux. Little is known, however, about the relationship between antroduodenal resistance and the physiological patterns of contractile activity in this area. We have developed an instrument that maintains an electronically regulated constant-pressure gradient of 2 mmHg across both ends of a flaccid cylinder positioned fluoroscopically across the pylorus. Because resistance bears a constant inverse relationship to flow at a fixed pressure gradient, changes in the recorded rate of airflow through the cylinder are a measure of antroduodenal resistance. In vitro studies showed that, under these conditions, airflow was a function of the diameter and length of the air path and the frequency and duration of external pressure waves greater than 2 mmHg. In vivo studies in four dogs examined the relationship between interdigestive phases of motor activity and variations in resistance exerted by the antroduodenal area. We found that flow rates varied markedly with each phase. Antroduodenal resistance was lowest during motor quiescence (phase I), rose gradually during irregular activity (phase II), and reached its peak during maximal contractile activity (phase III) (P less than 0.05). Resistance was similar for antegrade and retrograde flow. Additional studies suggested that the pyloric area contributes mostly to resistance during phase I, whereas duodenal resistance at least matches that of the pylorus during phase III.

  5. How to measure and monitor antimicrobial consumption and resistance.

    Science.gov (United States)

    Grau, Santiago; Bou, Germán; Fondevilla, Esther; Nicolás, Jordi; Rodríguez-Maresca, Manuel; Martínez-Martínez, Luis

    2013-09-01

    Collateral damage caused by antibiotic use includes resistance, which could be reduced if the global inappropriate use of antibiotics, especially in low-income countries, could be prevented. Surveillance of antimicrobial consumption can identify and target practice areas for quality improvement, both in the community and in healthcare institutions. The defined daily dose, the usual adult dose of an antimicrobial for treating one patient for one day, has been considered useful for measuring antimicrobial prescribing trends within a hospital. Various denominators from hospital activity including beds, admissions and discharges have been used to obtain some standard ratios for comparing antibiotic consumption between hospitals and countries. Laboratory information systems in Clinical Microbiology Services are the primary resource for preparing cumulative reports on susceptibility testing results. This information is useful for planning empirical treatment and for adopting infection control measures. Among the supranational initiatives on resistance surveillance, the EARS-Net provides information about trends on antimicrobial resistance in Europe. Resistance is the consequence of the selective pressure of antibiotics, although in some cases these agents also promote resistance by favouring the emergence of mutations that are subsequently selected. Multiple studies have shown a relationship between antimicrobial use and emergence or resistance. While in some cases a decrease in antibiotic use was associated with a reduction in resistance rates, in many other situations this has not been the case, due to co-resistance and/or the low biological cost of the resistance mechanisms involved. New antimicrobial agents are urgently needed, which coupled with infection control measures will help to control the current problem of antimicrobial resistance. Copyright © 2013 Elsevier España, S.L. All rights reserved.

  6. Methods for Sampling and Measurement of Compressed Air Contaminants

    Energy Technology Data Exchange (ETDEWEB)

    Stroem, L.

    1976-10-15

    In order to improve the technique for measuring oil and water entrained in a compressed air stream, a laboratory study has been made of some methods for sampling and measurement. For this purpose water or oil as artificial contaminants were injected in thin streams into a test loop, carrying dry compressed air. Sampling was performed in a vertical run, down-stream of the injection point. Wall attached liquid, coarse droplet flow, and fine droplet flow were sampled separately. The results were compared with two-phase flow theory and direct observation of liquid behaviour. In a study of sample transport through narrow tubes, it was observed that, below a certain liquid loading, the sample did not move, the liquid remaining stationary on the tubing wall. The basic analysis of the collected samples was made by gravimetric methods. Adsorption tubes were used with success to measure water vapour. A humidity meter with a sensor of the aluminium oxide type was found to be unreliable. Oil could be measured selectively by a flame ionization detector, the sample being pretreated in an evaporation- condensation unit

  7. Non-Contact Conductivity Measurement for Automated Sample Processing Systems

    Science.gov (United States)

    Beegle, Luther W.; Kirby, James P.

    2012-01-01

    A new method has been developed for monitoring and control of automated sample processing and preparation especially focusing on desalting of samples before analytical analysis (described in more detail in Automated Desalting Apparatus, (NPO-45428), NASA Tech Briefs, Vol. 34, No. 8 (August 2010), page 44). The use of non-contact conductivity probes, one at the inlet and one at the outlet of the solid phase sample preparation media, allows monitoring of the process, and acts as a trigger for the start of the next step in the sequence (see figure). At each step of the muti-step process, the system is flushed with low-conductivity water, which sets the system back to an overall low-conductivity state. This measurement then triggers the next stage of sample processing protocols, and greatly minimizes use of consumables. In the case of amino acid sample preparation for desalting, the conductivity measurement will define three key conditions for the sample preparation process. First, when the system is neutralized (low conductivity, by washing with excess de-ionized water); second, when the system is acidified, by washing with a strong acid (high conductivity); and third, when the system is at a basic condition of high pH (high conductivity). Taken together, this non-contact conductivity measurement for monitoring sample preparation will not only facilitate automation of the sample preparation and processing, but will also act as a way to optimize the operational time and use of consumables

  8. Variable screening and ranking using sampling-based sensitivity measures

    International Nuclear Information System (INIS)

    Wu, Y-T.; Mohanty, Sitakanta

    2006-01-01

    This paper presents a methodology for screening insignificant random variables and ranking significant important random variables using sensitivity measures including two cumulative distribution function (CDF)-based and two mean-response based measures. The methodology features (1) using random samples to compute sensitivities and (2) using acceptance limits, derived from the test-of-hypothesis, to classify significant and insignificant random variables. Because no approximation is needed in either the form of the performance functions or the type of continuous distribution functions representing input variables, the sampling-based approach can handle highly nonlinear functions with non-normal variables. The main characteristics and effectiveness of the sampling-based sensitivity measures are investigated using both simple and complex examples. Because the number of samples needed does not depend on the number of variables, the methodology appears to be particularly suitable for problems with large, complex models that have large numbers of random variables but relatively few numbers of significant random variables

  9. Characterization measurements of Ti-SS bimetallic transition joint samples

    International Nuclear Information System (INIS)

    Budagov, Yu.; Sabirov, B.; Sisakyan, A.

    2008-01-01

    A small set of bimetallic tubes has been investigated for the purpose to determine characteristics of samples at different conditions of tests for more statistics. Nine bimetallic samples have been manufactured at the Russian Federal Nuclear Center - VNIIEF (Sarov, Russia) using explosion technology for welding titanium and stainless steel tubes. During the tests eight samples have shown an excellent behaviour. This result is very good and we believe that these samples can be used for the construction of the cavity vessels. A preliminary measurement on the residual magnetic moment around junction line between the two materials has been carried out

  10. Consensus of Multiagent Systems with Sampled Information and Noisy Measurements

    Directory of Open Access Journals (Sweden)

    Zhao-Jun Tang

    2013-01-01

    Full Text Available We consider consensus problems of first-order multiagent systems with sampled information and noisy measurements. A distributed stochastic approximation type algorithm is employed to attenuate the measurement noises. We provide conditions under which almost sure strong consensus is guaranteed for fixed and switching directed network topologies. Simulation results are provided to illustrate the theoretical results.

  11. Measurement Error Calibration in Mixed-Mode Sample Surveys

    Science.gov (United States)

    Buelens, Bart; van den Brakel, Jan A.

    2015-01-01

    Mixed-mode surveys are known to be susceptible to mode-dependent selection and measurement effects, collectively referred to as mode effects. The use of different data collection modes within the same survey may reduce selectivity of the overall response but is characterized by measurement errors differing across modes. Inference in sample surveys…

  12. Rapid methods for measuring radionuclides in food and environmental samples

    International Nuclear Information System (INIS)

    Perkins, Richard W.

    1995-01-01

    The application of ICP/mass spectrometry for the isotopic analysis of environmental samples, the use of drum assayers for measuring radionuclides in food and a rapid procedure for the measurement of the transuranic elements and thorium, performed at the Pacific Northwest Laboratory are discussed

  13. Friction coefficient determination by electrical resistance measurements

    Science.gov (United States)

    Tunyagi, A.; Kandrai, K.; Fülöp, Z.; Kapusi, Z.; Simon, A.

    2018-05-01

    A simple and low-cost, DIY-type, Arduino-driven experiment is presented for the study of friction and measurement of the friction coefficient, using a conductive rubber cord as a force sensor. It is proposed for high-school or college/university-level students. We strongly believe that it is worthwhile planning, designing and performing Arduino and compatible sensor-based experiments in physics class in order to ensure a better understanding of phenomena, develop theoretical knowledge and multiple experimental skills.

  14. Traceability and measurement uncertainty in sample preparation (W5)

    International Nuclear Information System (INIS)

    Wegscheider, W.; Walner, U.; Moser, J.

    2002-01-01

    Full text: Very few chemical measurements are being made directly on the object of interest and sample preparation is thus the rule rather than the exception in daily practice. Unfortunately the operations undertaken in the course of sample preparation are prone to rendering a sample useless for the purpose of interpreting a measurement performed on it, as it might not represent the original and relevant status any longer. Sample preparation along with sampling itself constitutes therefore a procedure that leads to a loss of representation of the original specimen or population. On the other hand it is also not sufficient to confine aspects of traceability and measurement uncertainty to the ultimate measurement, as the key purpose of measuring is to supply adequate data for some kind of decision, be it in production, in health, in the environment, or indeed in any other circumstance. These considerations have led to severe confusion in the community as to what traceability really means in chemistry. CITAC and EURACHEM have only recently issued a preliminary document that clarifies these issues and gives a firm handle on the future development of quality assurance in analytical chemistry. In this talk it will be attempted to outline the general ideas and procedures that lead to traceability of analytical chemical results accompanied by valid statements of their uncertainty. It will be argued that the central element in achieving these goals is a well-designed validation study that frequently goes beyond those requirements currently laid out in official documents. (author)

  15. Analytical Performance of Multiplexed Screening Test for 10 Antibiotic Resistance Genes from Perianal Swab Samples.

    Science.gov (United States)

    Walker, G Terrance; Rockweiler, Tony J; Kersey, Rossio K; Frye, Kelly L; Mitchner, Susan R; Toal, Douglas R; Quan, Julia

    2016-02-01

    Multiantibiotic-resistant bacteria pose a threat to patients and place an economic burden on health care systems. Carbapenem-resistant bacilli and extended-spectrum β-lactamase (ESBL) producers drive the need to screen infected and colonized patients for patient management and infection control. We describe a multiplex microfluidic PCR test for perianal swab samples (Acuitas(®) MDRO Gene Test, OpGen) that detects the vancomycin-resistance gene vanA plus hundreds of gene subtypes from the carbapenemase and ESBL families Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-β-lactamase (NDM), Verona integron-mediated metallo-β-lactamase (VIM), imipenemase metallo-β-lactamase (IMP), OXA-23, OXA-48, OXA-51, CTX-M-1, and CTX-M-2, regardless of the bacterial species harboring the antibiotic resistance. Analytical test sensitivity per perianal swab is 11-250 CFU of bacteria harboring the antibiotic resistance genes. Test throughput is 182 samples per test run (1820 antibiotic resistance gene family results). We demonstrate reproducible test performance and 100% gene specificity for 265 clinical bacterial organisms harboring a variety of antibiotic resistance genes. The Acuitas MDRO Gene Test is a sensitive, specific, and high-throughput test to screen colonized patients and diagnose infections for several antibiotic resistance genes directly from perianal swab samples, regardless of the bacterial species harboring the resistance genes. © 2015 American Association for Clinical Chemistry.

  16. Acute Effects of 30 Minutes Resistance and Aerobic Exercise on Cognition in a High School Sample

    Science.gov (United States)

    Harveson, Andrew T.; Hannon, James C.; Brusseau, Timothy A.; Podlog, Leslie; Papadopoulos, Charilaos; Durrant, Lynne H.; Hall, Morgan S.; Kang, Kyoung-doo

    2016-01-01

    Purpose: The purpose of this study was to determine differences in cognition between acute bouts of resistance exercise, aerobic exercise, and a nonexercise control in an untrained youth sample. Method: Ninety-four participants performed 30 min of aerobic exercise, resistance exercise, or nonexercise separated by 7 days each in a randomized…

  17. Sensitivity of resistive and Hall measurements to local inhomogeneities

    DEFF Research Database (Denmark)

    Koon, Daniel W.; Wang, Fei; Petersen, Dirch Hjorth

    2014-01-01

    We derive exact, analytic expressions for the sensitivity of sheet resistance and Hall sheet resistance measurements to local inhomogeneities for the cases of nonzero magnetic fields, strong perturbations, and perturbations over a finite area, extending our earlier results on weak perturbations. ...... simulations on both a linear four-point probe array on a large circular disc and a van der Pauw square geometry. Furthermore, the results also agree well with Náhlík et al. published experimental results for physical holes in a circular copper foil disc.......We derive exact, analytic expressions for the sensitivity of sheet resistance and Hall sheet resistance measurements to local inhomogeneities for the cases of nonzero magnetic fields, strong perturbations, and perturbations over a finite area, extending our earlier results on weak perturbations. We...

  18. Use of Lot Quality Assurance Sampling to Ascertain Levels of Drug Resistant Tuberculosis in Western Kenya.

    Science.gov (United States)

    Jezmir, Julia; Cohen, Ted; Zignol, Matteo; Nyakan, Edwin; Hedt-Gauthier, Bethany L; Gardner, Adrian; Kamle, Lydia; Injera, Wilfred; Carter, E Jane

    2016-01-01

    To classify the prevalence of multi-drug resistant tuberculosis (MDR-TB) in two different geographic settings in western Kenya using the Lot Quality Assurance Sampling (LQAS) methodology. The prevalence of drug resistance was classified among treatment-naïve smear positive TB patients in two settings, one rural and one urban. These regions were classified as having high or low prevalence of MDR-TB according to a static, two-way LQAS sampling plan selected to classify high resistance regions at greater than 5% resistance and low resistance regions at less than 1% resistance. This study classified both the urban and rural settings as having low levels of TB drug resistance. Out of the 105 patients screened in each setting, two patients were diagnosed with MDR-TB in the urban setting and one patient was diagnosed with MDR-TB in the rural setting. An additional 27 patients were diagnosed with a variety of mono- and poly- resistant strains. Further drug resistance surveillance using LQAS may help identify the levels and geographical distribution of drug resistance in Kenya and may have applications in other countries in the African Region facing similar resource constraints.

  19. Use of Lot Quality Assurance Sampling to Ascertain Levels of Drug Resistant Tuberculosis in Western Kenya.

    Directory of Open Access Journals (Sweden)

    Julia Jezmir

    Full Text Available To classify the prevalence of multi-drug resistant tuberculosis (MDR-TB in two different geographic settings in western Kenya using the Lot Quality Assurance Sampling (LQAS methodology.The prevalence of drug resistance was classified among treatment-naïve smear positive TB patients in two settings, one rural and one urban. These regions were classified as having high or low prevalence of MDR-TB according to a static, two-way LQAS sampling plan selected to classify high resistance regions at greater than 5% resistance and low resistance regions at less than 1% resistance.This study classified both the urban and rural settings as having low levels of TB drug resistance. Out of the 105 patients screened in each setting, two patients were diagnosed with MDR-TB in the urban setting and one patient was diagnosed with MDR-TB in the rural setting. An additional 27 patients were diagnosed with a variety of mono- and poly- resistant strains.Further drug resistance surveillance using LQAS may help identify the levels and geographical distribution of drug resistance in Kenya and may have applications in other countries in the African Region facing similar resource constraints.

  20. The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches

    DEFF Research Database (Denmark)

    Dalhoff Andersen, Vibe; de Knegt, Leonardo; Munk, Patrick

    2017-01-01

    The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10...... finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation...... effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use...

  1. Spatial scan statistics to assess sampling strategy of antimicrobial resistance monitoring programme

    DEFF Research Database (Denmark)

    Vieira, Antonio; Houe, Hans; Wegener, Henrik Caspar

    2009-01-01

    Pie collection and analysis of data on antimicrobial resistance in human and animal Populations are important for establishing a baseline of the occurrence of resistance and for determining trends over time. In animals, targeted monitoring with a stratified sampling plan is normally used. However...... sampled by the Danish Integrated Antimicrobial Resistance Monitoring and Research Programme (DANMAP), by identifying spatial Clusters of samples and detecting areas with significantly high or low sampling rates. These analyses were performed for each year and for the total 5-year study period for all...... collected and Susceptibility tested pig samples in Denmark between 2002 and 2006. For the yearly analysis, both high and low sampling rates areas were significant, with two clusters in 2002 (relative risk [RR]: 2.91, p

  2. Population Screening Using Sewage Reveals Pan-Resistant Bacteria in Hospital and Community Samples.

    Science.gov (United States)

    Meir-Gruber, Lital; Manor, Yossi; Gefen-Halevi, Shiraz; Hindiyeh, Musa Y; Mileguir, Fernando; Azar, Roberto; Smollan, Gill; Belausov, Natasha; Rahav, Galia; Shamiss, Ari; Mendelson, Ella; Keller, Nathan

    2016-01-01

    The presence of pan-resistant bacteria worldwide possesses a threat to global health. It is difficult to evaluate the extent of carriage of resistant bacteria in the population. Sewage sampling is a possible way to monitor populations. We evaluated the presence of pan-resistant bacteria in Israeli sewage collected from all over Israel, by modifying the pour plate method for heterotrophic plate count technique using commercial selective agar plates. This method enables convenient and fast sewage sampling and detection. We found that sewage in Israel contains multiple pan-resistant bacteria including carbapenemase resistant Enterobacteriacae carrying blaKPC and blaNDM-1, MRSA and VRE. blaKPC carrying Klebsiella pneumonia and Enterobacter cloacae were the most common Enterobacteriacae drug resistant bacteria found in the sewage locations we sampled. Klebsiella pneumonia, Enterobacter spp., Escherichia coli and Citrobacter spp. were the 4 main CRE isolated from Israeli sewage and also from clinical samples in our clinical microbiology laboratory. Hospitals and Community sewage had similar percentage of positive samplings for blaKPC and blaNDM-1. VRE was found to be more abundant in sewage in Israel than MRSA but there were more locations positive for MRSA and VRE bacteria in Hospital sewage than in the Community. Therefore, our upgrade of the pour plate method for heterotrophic plate count technique using commercial selective agar plates can be a useful tool for routine screening and monitoring of the population for pan-resistant bacteria using sewage.

  3. Measurement of resistivity changes in irradiated microscopy discs

    International Nuclear Information System (INIS)

    Sagisaka, M.; Isobe, Y.; Edwards, D.J.; Garner, F.; Okita, T.

    2007-01-01

    Full text of publication follows: The successful operation of next generation fusion or fission devices will require the development of new inspection tools to allow in-situ, non-destructive examination of structural components which experience the deleterious effects of neutron irradiation. Such development requires that an understanding of how radiation-induced microstructural alteration contributes to macroscopic changes in physical properties such as electrical resistivity. This in turn requires test specimens spanning a range of microstructural alteration. Frequently such specimens are very small and available test techniques are not suitable for their examination. An example is the use of thin TEM specimens (3 mm diameter, 0.3 mm thick) used for electron microscopy. A unique four probe electrical resistivity measurement system suitable for examining I EM specimens was developed for investigating small resistivity changes due to void swelling and other microstructural features. Since this system uses momentarily-high electrical currents (0.5 A maximum), electrical resistivity changes can be measured rather precisely. This paper reports results of resistivity change measurements made on model Fe-Cr-Ni-Zr austenitic alloys irradiated in the Fast Flux Test Facility in the Materials Open Test Assembly to doses ranging from 0.38 to 19.2 dpa. Microscopy was used to determine the radiation-induced microstructure. A correlation is presented for resistivity changes arising primarily from void swelling. (authors)

  4. Rapid determination of bacterial aminoglycoside resistance in environmental samples using membrane electrospray ionization mass spectrometry.

    Science.gov (United States)

    Fan, Liusheng; Ke, Ming; Yuan, Min; Pu, Ji; Li, Juan; Lu, Jinxing; Xu, Jianguo; Zhang, Mei; Xu, Wei

    2016-08-01

    Antibiotic resistance in pathogenic bacteria is becoming a global public health problem, such as aminoglycoside resistance encoded by the armA gene. Although many methods have been reported, rapid analysis of environmental samples is still challenging. A rapid analytical method was developed in this study to determine bacterial aminoglycoside resistance using membrane electrospray ionization mass spectrometry (MESI-MS). Precursor/product-ion pairs of ArmA unique peptides were detected with minimal sample preparation. Standard peptides were synthesized and used for developing and validating the methodology, and then the method was verified by both ArmA positive and ArmA negative simulated environmental samples. A rapid method for determination of bacterial aminoglycoside resistance was developed using MESI-MS/MS. The bacterial cultural time was optimized to 2 hours, and the precision, accuracy and recovery of this method were investigated. The peptide IHSSTNER (IR-8) unique to ArmA in simulated environmental samples can be successfully identified within 3 hours. The novel assay offered a rapid method to determine bacterial aminoglycoside resistance with high sensitivity, accuracy and precision in simulated environmental samples. This method could also be applied to identify other drug-resistance proteins in clinical/environmental samples. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  5. Study of probe-sample distance for biomedical spectra measurement

    Directory of Open Access Journals (Sweden)

    Li Lei

    2011-11-01

    Full Text Available Abstract Background Fiber-based optical spectroscopy has been widely used for biomedical applications. However, the effect of probe-sample distance on the collection efficiency has not been well investigated. Method In this paper, we presented a theoretical model to maximize the illumination and collection efficiency in designing fiber optic probes for biomedical spectra measurement. This model was in general applicable to probes with single or multiple fibers at an arbitrary incident angle. In order to demonstrate the theory, a fluorescence spectrometer was used to measure the fluorescence of human finger skin at various probe-sample distances. The fluorescence spectrum and the total fluorescence intensity were recorded. Results The theoretical results show that for single fiber probes, contact measurement always provides the best results. While for multi-fiber probes, there is an optimal probe distance. When a 400- μm excitation fiber is used to deliver the light to the skin and another six 400- μm fibers surrounding the excitation fiber are used to collect the fluorescence signal, the experimental results show that human finger skin has very strong fluorescence between 475 nm and 700 nm under 450 nm excitation. The fluorescence intensity is heavily dependent on the probe-sample distance and there is an optimal probe distance. Conclusions We investigated a number of probe-sample configurations and found that contact measurement could be the primary choice for single-fiber probes, but was very inefficient for multi-fiber probes. There was an optimal probe-sample distance for multi-fiber probes. By carefully choosing the probe-sample distance, the collection efficiency could be enhanced by 5-10 times. Our experiments demonstrated that the experimental results of the probe-sample distance dependence of collection efficiency in multi-fiber probes were in general agreement with our theory.

  6. Infrared nondestructive measurement of thermal resistance between liner and engine block: design of experiment

    Energy Technology Data Exchange (ETDEWEB)

    Laloue, P.; L' Ecolier, J.; Nigon, F. [PSA Peugeot Citroen, Laboratoire Optique et Thermique, 45 rue Jean-Pierre Timbaud, 78 300 Poissy (France); Bissieux, C.; Henry, J.-F.; Pron, H. [Universite de Reims, Unite de Thermique et Analyse Physique, EA 3802, Laboratoire de Thermophysique, UFR Sciences, Moulin de la Housse, BP 1039, 51 687 Reims Cedex 2 (France)

    2008-03-15

    Thermal resistances between liners and engine blocks are nondestructively studied by photothermal infrared thermography. Under controlled sinusoidal light irradiation, the thermal response of the sample is measured by means of an infrared camera. A numerical lock-in procedure yields amplitude and absolute phase maps of the thermal field periodic component. Then, apart from classical qualitative detection of air layers, a quantitative characterization of thermal resistance becomes available. An analytical modeling, associated with an inverse procedure using the Gauss-Newton parameter estimation method, allows to identify the thermal resistance on academic samples representative of the liner-engine block interface. Simply joined cast iron and aluminum plates present thermal resistances about 2 x 10{sup -3} K m{sup 2} W{sup -1}. The implementation of a numerical modeling allows to study two-dimensional defects. When the samples are pressed on their periphery, thus straightened, contact resistances ranging from 2 x 10{sup -4} to 7 x 10{sup -4} K m{sup 2} W{sup -1} have been measured. Then, the method is applied to liner-engine block interfaces where the thermal resistances fall to about 2 x 10{sup -5} K m{sup 2} W{sup -1}, matching the values obtained when a cast iron plate is locally pressed against an aluminum plate. (author)

  7. Multilayer Steel Materials Deformation Resistance and Roll Force Measurement

    Directory of Open Access Journals (Sweden)

    A. G. Kolesnikov

    2014-01-01

    Full Text Available To create new types of cars, raise their reliability, gain operational life, and decrease in metal consumption of products it is necessary to improve mechanical, physical, and also special properties of the constructional materials applied in mechanical engineering. Presently, there are intensive researches and developments under way to create materials with ultrafine-grained structure (the sizes of grains in their crystal lattice make less than 1 micron in one of the measurements.BMSTU developed a manufacturing technology of multilayer steel sheets with steady ultrafine-grained structure based on the multiple hot rolling of billet as a composition consisting of the alternating metal sheets. A principled condition for implementation of such technology is existence of different crystallographic modifications in the adjoining sheets of the composition at specified temperature of rolling.Power parameters of rolling are important technical characteristics of the process. Usually, to determine a deformation resistance value when rolling the diverse multilayer materials, is used the actual resistance value averaging in relation to the components of the composition. The aim of this work is a comparative analysis of known calculated dependences with experimental data when rolling the 100-layer samples. Objects of research were the 100-layer compositions based on the alternating layers of steel 08H18N10 and U8.Experimental samples represented the vacuumized capsules with height, width, and length of 53 mm x 53 mm x 200 mm, respectively, in which there were the 100-layer packs from sheets, each of 0.5 mm, based on the composition of steels (U8+08H18N10. Rolling was made on the double-high mill with rolls of 160 mm in diameter during 19 passes to the thickness of 7 mm with the speed of 0,1 m/s. Relative sinking in each pass was accepted to be equal 10±2,5%. Rolling forces were measured by the strain-gauging method using the measuring cells, located under

  8. Heat-capacity measurements on small samples: The hybrid method

    NARCIS (Netherlands)

    Klaasse, J.C.P.; Brück, E.H.

    2008-01-01

    A newly developed method is presented for measuring heat capacities on small samples, particularly where thermal isolation is not sufficient for the use of the traditional semiadiabatic heat-pulse technique. This "hybrid technique" is a modification of this heat-pulse method in case the temperature

  9. A sampling and metagenomic sequencing-based methodology for monitoring antimicrobial resistance in swine herds

    DEFF Research Database (Denmark)

    Munk, Patrick; Dalhoff Andersen, Vibe; de Knegt, Leonardo

    2016-01-01

    Objectives Reliable methods for monitoring antimicrobial resistance (AMR) in livestock and other reservoirs are essential to understand the trends, transmission and importance of agricultural resistance. Quantification of AMR is mostly done using culture-based techniques, but metagenomic read...... on known antimicrobial consumption in 10 Danish integrated slaughter pig herds. In addition, we evaluated whether fresh or manure floor samples constitute suitable proxies for intestinal sampling, using cfu counting, qPCR and metagenomic shotgun sequencing. Results Metagenomic read-mapping outperformed...... cultivation-based techniques in terms of predicting expected tetracycline resistance based on antimicrobial consumption. Our metagenomic approach had sufficient resolution to detect antimicrobial-induced changes to individual resistance gene abundances. Pen floor manure samples were found to represent rectal...

  10. Multidrug resistance among new tuberculosis cases: detecting local variation through lot quality-assurance sampling.

    Science.gov (United States)

    Hedt, Bethany Lynn; van Leth, Frank; Zignol, Matteo; Cobelens, Frank; van Gemert, Wayne; Nhung, Nguyen Viet; Lyepshina, Svitlana; Egwaga, Saidi; Cohen, Ted

    2012-03-01

    Current methodology for multidrug-resistant tuberculosis (MDR TB) surveys endorsed by the World Health Organization provides estimates of MDR TB prevalence among new cases at the national level. On the aggregate, local variation in the burden of MDR TB may be masked. This paper investigates the utility of applying lot quality-assurance sampling to identify geographic heterogeneity in the proportion of new cases with multidrug resistance. We simulated the performance of lot quality-assurance sampling by applying these classification-based approaches to data collected in the most recent TB drug-resistance surveys in Ukraine, Vietnam, and Tanzania. We explored 3 classification systems- two-way static, three-way static, and three-way truncated sequential sampling-at 2 sets of thresholds: low MDR TB = 2%, high MDR TB = 10%, and low MDR TB = 5%, high MDR TB = 20%. The lot quality-assurance sampling systems identified local variability in the prevalence of multidrug resistance in both high-resistance (Ukraine) and low-resistance settings (Vietnam). In Tanzania, prevalence was uniformly low, and the lot quality-assurance sampling approach did not reveal variability. The three-way classification systems provide additional information, but sample sizes may not be obtainable in some settings. New rapid drug-sensitivity testing methods may allow truncated sequential sampling designs and early stopping within static designs, producing even greater efficiency gains. Lot quality-assurance sampling study designs may offer an efficient approach for collecting critical information on local variability in the burden of multidrug-resistant TB. Before this methodology is adopted, programs must determine appropriate classification thresholds, the most useful classification system, and appropriate weighting if unbiased national estimates are also desired.

  11. Sampling Approaches for Multi-Domain Internet Performance Measurement Infrastructures

    Energy Technology Data Exchange (ETDEWEB)

    Calyam, Prasad

    2014-09-15

    The next-generation of high-performance networks being developed in DOE communities are critical for supporting current and emerging data-intensive science applications. The goal of this project is to investigate multi-domain network status sampling techniques and tools to measure/analyze performance, and thereby provide “network awareness” to end-users and network operators in DOE communities. We leverage the infrastructure and datasets available through perfSONAR, which is a multi-domain measurement framework that has been widely deployed in high-performance computing and networking communities; the DOE community is a core developer and the largest adopter of perfSONAR. Our investigations include development of semantic scheduling algorithms, measurement federation policies, and tools to sample multi-domain and multi-layer network status within perfSONAR deployments. We validate our algorithms and policies with end-to-end measurement analysis tools for various monitoring objectives such as network weather forecasting, anomaly detection, and fault-diagnosis. In addition, we develop a multi-domain architecture for an enterprise-specific perfSONAR deployment that can implement monitoring-objective based sampling and that adheres to any domain-specific measurement policies.

  12. How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps

    Directory of Open Access Journals (Sweden)

    Jessica M. A. Blair

    2016-07-01

    Full Text Available Bacterial multidrug resistance (MDR efflux pumps are an important mechanism of antibiotic resistance and are required for many pathogens to cause infection. They are also being harnessed to improve microbial biotechnological processes, including biofuel production. Therefore, scientists of many specialties must be able to accurately measure efflux activity. However, myriad methodologies have been described and the most appropriate method is not always clear. Within the scientific literature, many methods are misused or data arising are misinterpreted. The methods for measuring efflux activity can be split into two groups, (i those that directly measure efflux and (ii those that measure the intracellular accumulation of a substrate, which is then used to infer efflux activity. Here, we review the methods for measuring efflux and explore the most recent advances in this field, including single-cell or cell-free technologies and mass spectrometry, that are being used to provide more detailed information about efflux pump activity.

  13. An empirical comparison of isolate-based and sample-based definitions of antimicrobial resistance and their effect on estimates of prevalence.

    Science.gov (United States)

    Humphry, R W; Evans, J; Webster, C; Tongue, S C; Innocent, G T; Gunn, G J

    2018-02-01

    Antimicrobial resistance is primarily a problem in human medicine but there are unquantified links of transmission in both directions between animal and human populations. Quantitative assessment of the costs and benefits of reduced antimicrobial usage in livestock requires robust quantification of transmission of resistance between animals, the environment and the human population. This in turn requires appropriate measurement of resistance. To tackle this we selected two different methods for determining whether a sample is resistant - one based on screening a sample, the other on testing individual isolates. Our overall objective was to explore the differences arising from choice of measurement. A literature search demonstrated the widespread use of testing of individual isolates. The first aim of this study was to compare, quantitatively, sample level and isolate level screening. Cattle or sheep faecal samples (n=41) submitted for routine parasitology were tested for antimicrobial resistance in two ways: (1) "streak" direct culture onto plates containing the antimicrobial of interest; (2) determination of minimum inhibitory concentration (MIC) of 8-10 isolates per sample compared to published MIC thresholds. Two antibiotics (ampicillin and nalidixic acid) were tested. With ampicillin, direct culture resulted in more than double the number of resistant samples than the MIC method based on eight individual isolates. The second aim of this study was to demonstrate the utility of the observed relationship between these two measures of antimicrobial resistance to re-estimate the prevalence of antimicrobial resistance from a previous study, in which we had used "streak" cultures. Boot-strap methods were used to estimate the proportion of samples that would have tested resistant in the historic study, had we used the isolate-based MIC method instead. Our boot-strap results indicate that our estimates of prevalence of antimicrobial resistance would have been

  14. Sample variance in the local measurements of the Hubble constant

    Science.gov (United States)

    Wu, Hao-Yi; Huterer, Dragan

    2017-11-01

    The current >3σ tension between the Hubble constant H0 measured from local distance indicators and from cosmic microwave background is one of the most highly debated issues in cosmology, as it possibly indicates new physics or unknown systematics. In this work, we explore whether this tension can be alleviated by the sample variance in the local measurements, which use a small fraction of the Hubble volume. We use a large-volume cosmological N-body simulation to model the local measurements and to quantify the variance due to local density fluctuations and sample selection. We explicitly take into account the inhomogeneous spatial distribution of type Ia supernovae. Despite the faithful modelling of the observations, our results confirm previous findings that sample variance in the local Hubble constant (H_0^loc) measurements is small; we find σ (H_0^loc)=0.31 {km s^{-1}Mpc^{-1}}, a nearly negligible fraction of the ˜6 km s-1Mpc-1 necessary to explain the difference between the local and global H0 measurements. While the H0 tension could in principle be explained by our local neighbourhood being a underdense region of radius ˜150 Mpc, the extreme required underdensity of such a void (δ ≃ -0.8) makes it very unlikely in a ΛCDM universe, and it also violates existing observational constraints. Therefore, sample variance in a ΛCDM universe cannot appreciably alleviate the tension in H0 measurements even after taking into account the inhomogeneous selection of type Ia supernovae.

  15. A high temperature cell for simultaneous electrical resistance and neutron diffraction measurements.

    Science.gov (United States)

    Engin, T E; Powell, A V; Haynes, R; Chowdhury, M A H; Goodway, C M; Done, R; Kirichek, O; Hull, S

    2008-09-01

    An in situ cell that allows the electrical resistance of a sample pellet to be measured while performing neutron diffraction experiments has been developed at the ISIS pulsed neutron source. The sample is held between two spring loaded platinum electrodes embedded in a boron nitride clamp assembly with the resistance measured using the four-probe method. An outer quartz glass jacket allows the atmosphere within the sample enclosure to be controlled, and the entire device can be accommodated within a standard ISIS neutron furnace for measurements at temperatures up to 1270 K. The operation of this cell is illustrated using data for the structural, magnetic, and electrical properties of chalcopyrite CuFeS(2) collected over the temperature range of 398-873 K on the Polaris powder diffractometer at ISIS.

  16. Standard test method for measurement of soil resistivity using the two-electrode soil box method

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2005-01-01

    1.1 This test method covers the equipment and a procedure for the measurement of soil resistivity, for samples removed from the ground, for use in the control of corrosion of buried structures. 1.2 Procedures allow for this test method to be used n the field or in the laboratory. 1.3 The test method procedures are for the resistivity measurement of soil samples in the saturated condition and in the as-received condition. 1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only. Soil resistivity values are reported in ohm-centimeter. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.

  17. The measurement of radioactive microspheres in biological samples

    International Nuclear Information System (INIS)

    Mernagh, J.R.; Spiers, E.W.; Adiseshiah, M.

    1976-01-01

    Measurements of the distribution of radioactive microspheres are used in investigations of regional coronary blood flow, but the size and shape of the heart varies for different test animals, and the organ is frequently divided into smaller pieces for studies of regional perfusion. Errors are introduced by variations in the distribution of the radioactive source and the amount of Compton scatter in different samples. A technique has therefore been developed to allow the counting of these tissue samples in their original form, and correction factors have been derived to inter-relate the various counting geometries thus encountered. Dogs were injected with microspheres labelled with 141 Ce, 51 Cr or 85 Sr. The tissue samples did not require remodelling to fit a standard container, and allowance was made for the inhomogeneous distribution in the blood samples. The activities in the centrifuged blood samples were correlated with those from the tissue samples by a calibration procedure involving comparisons of the counts from samples of microspheres embedded in sachets of gelatine, and similar samples mixed with blood and then centrifuged. The calibration data have indicated that 51 Cr behaves anomalously, and its use as a label for microspheres may introduce unwarranted errors. A plane cylindrical 10 x 20 cm NaI detector was used, and a 'worst case' correction of 20% was found to be necessary for geometry effects. The accuracy of this method of correlating different geometries was tested by remodelling the same tissue sample into different sizes and comparing the results, and the validity of the technique was supported by agreement of the final results with previously published data. (U.K.)

  18. Antibiotic resistance in bacteria Staphylococcus spp. isolated from samples of raw sheep's milk

    Directory of Open Access Journals (Sweden)

    Milan Vasiľ

    2016-12-01

    Full Text Available From samples of raw sheep's milk were determined results of bacteriological examination from two herds in region of Eastern Slovakia in three years lasting study. The occurrence of Staphylococcus spp. 41.6% (124 was determined from 298 samples. The seven species of staphylococci were on a regular basis isolated: S. epidermidis (34, S. chromogenes (26, S. aureus (16. Alternately have been recorded S. warneri (16, S. schleiferi (15, S. haemolyticus (9 and S. xylosus (8. All isolated pathogens were tested by in vitro test on Mueller-Hinton agar by disc methods on resistance to 10 types of antibiotics.  Highest value of resistance was determined to Penicilin 21.0%, Neomycin 10.5% and Novobiocin 9.7%. Lower resistance was in to Oxacilin 7.2% and Amoxicilin 6.5%. Minimal resistance was founded to Cefoxitin 0.8%, Linkomycin 2.4%, Erytromycin, and Streptomycin 3.2%. Was founded total resistance (21.0% to all antibiotics in S. epidermidis (34 during the three years, S. chromogenes (26 showed resistance to 8 types of antibiotics (12.9%, S. aureus (16 to 6 antibiotics (10.5% and S. warneri (16 to 4 antibiotics (5.6%. It was confirmed that sheep's milk remains a major source of staphylococci. Bacteria in comparison with isolates from cows' raw milk, showed lower values of resistance, but were resistant to more than two antibiotics. Recorded occurrence of resistance in staphylococci may be connected with a minimum use of antibiotics in the treatment of mastitis and other diseases in sheep herds. Reported resistance to the tested antibiotics became the basis for the recommendation to use preparations to treat mastitis in sheep principally by the detection of resistance to antibiotics contained.

  19. PCR Screening of Antibiotic Resistance Genes in Faecal Samples from Australian and Chinese Children.

    Science.gov (United States)

    Ravensdale, Joshua T; Xian, Darren Ten Wei; Wei, Chooi Ming; Lv, Quanjun; Wen, Xiajian; Guo, Jing; Coorey, Ranil; LeSouëf, Peter; Lu, Fengmin; Zhang, Brad; Dykes, Gary A

    2018-03-31

    Recent public awareness campaigns on the risk of antibiotic resistance in pathogenic microbes has placed pressure on governments to enforce stricter antimicrobial stewardship policies on the hospital and agricultural industry. This study aimed to screen faecal samples from Australian and Chinese children for the presence of antibiotic resistance genes to identify demographics at risk of carriage of these genes and examine antimicrobial stewardship policies from the two countries which may influence carriage. Faecal samples from 46 Australian and 53 Chinese children were screened for the presence of six clinically relevant antibiotic resistance genes using PCR. Clinical and demographic data was also collected from each patient. Over 90% of faecal samples from Chinese children tested positive for β-lactam, macrolide, tetracycline, and aminoglycoside resistance genes, which was substantially higher than Australian samples. Besides country of origin, no clear trend could be seen to predict carriage of resistance genes. The exception to this was Chinese born children who immigrated to Australia having higher rates of carriage for bla TEM and tetM genes than children born and still living in Australia. These data indicated that Chinese children were more likely to carry certain antibiotic resistance genes than Australian children. The Chinese government has recently implemented strict policies to control the overuse of antibiotics in hospitals. However, many of these policies do not extend to the agricultural industry which could explain the differences seen in this study. Copyright © 2018. Published by Elsevier Ltd.

  20. Modelling of electrical resistance of semiconductive polymer pressed sample at the uniaxial compression

    International Nuclear Information System (INIS)

    Karimov, Kh.S.; Radzhabov, A.K.; Akhmedov, Yh.; Valiev, J.; Homidov, I.

    1999-01-01

    In the study the electrical resistance of pressed samples of the poly-N-methylcarbazole complex with iodine (PNMC) under uniaxial compression has been investigated by modeling. Physical model of the samples is considered in the form of contiguous of semiconducting spheres grains. It was investigated the influence of change of geometrical dimensions of the sample and electrical conductivity to resistance at the compression. For description of the polymers conductivity the hoping polaron mechanism of charge transfer was used. In the result of experimental data analysis, mathematical and physical models have been established: constant errors of the experiments are negligible in the linear dependence of the relative resistance on uniaxial pressures; tensity resistive effect caused by change of geometrical dimensions of the sample at the compression is less in comparison with increase of conductivity; the increase of the PMNC conductivity at the uniaxial compression may be result by increase of polaron's radius and dielectric permeability and decrease of polaron's jump length too. Received formula of the relative longitudinal resistance dependence on uruiaxial pressure may be used at the analysis of tensity resistive effect. (author)

  1. Spatial variability of soil penetration resistance influenced by season of sampling

    Directory of Open Access Journals (Sweden)

    Juan José Bonnin

    2010-01-01

    Full Text Available The aim of this work was to analyze the spatial distribution of soil compaction and the influence of soil water content on the resistance to penetration. The latter variable was described by the cone index. The soil at the study site was a Nitisol and the cone index data were obtained using a penetrometer. Soil resistance was assessed at 5 different depths, i.e. 0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm and deeper than 40 cm, whereas soil water content was measured at 0-20 cm and 20-40 cm. Soil water conditions varied during the different samplings. Coefficients of variation for cone index ranged from 16.5% to 45.8% while those for soil water content varied from 8.96% to 21.38%. Results suggested a high correlation between soil resistance, as assessed by the cone index, and soil depth. However, the expected relation with soil water content was not observed. Spatial dependence was observed in 31 out of 35 data series, both cone index and soil water content. This structure was fitted to exponential models with nugget effect varying from 0 to 90% of the sill value. Four of the data series showed a random behaviour. Inverse distance technique was used in order to map the distribution of the variables when no spatial structure was observed. Ordinary kriging showed a smoothing of the maps compared to those from inverse distance weighing. Indicator kriging was used to map the cone index spatial distribution for recommendation of further soil management.

  2. A diffusive sampling device for measurement of ammonia in air

    Science.gov (United States)

    Yamada, Tomomi; Uchiyama, Shigehisa; Inaba, Yohei; Kunugita, Naoki; Nakagome, Hideki; Seto, Hiroshi

    2012-07-01

    A diffusive sampling device, the DSD-NH3, has been developed for measurement of ammonia in air. The DSD-NH3 comprises silica gel impregnated with phosphoric acid as the absorbent, a porous sintered polyethylene tube that acts as a diffusive membrane, and a small polypropylene syringe that is used for the elution of analytes from the absorbent. Silica gel impregnated with phosphoric acid is used as absorbent for the DSD-NH3; basic gases in ambient air, including ammonia, are trapped in the DSD-NH3 device by their reaction with phosphoric acid in the sampler to form their corresponding phosphoric acid salts. After collection, the DSD-NH3 samplers are eluted by water. Cations in the eluate, including ammonium ions, are analyzed by ion chromatography. A side-by-side comparison was made with active samplers, demonstrating good correlation (r2 = 0.996). The sampling rate (94.5 ml min-1) was determined from comparison with an active sampling method and sampling rates. The sampling rate is also calculated from the respective molecular weights according to a rule based on Graham's law. The theoretical sampling rate with the DSD-NH3 is 95.4 ml min-1 and agrees with the experimental value (94.5 ml min-1). Little influence of wind velocity on the sampler was observed. The relative standard deviations for ammonia concentrations were 4.7% with face velocity ranging 0-5.0 m/s.

  3. Laboratory Measurement of the Electrical Resistivity of some ...

    African Journals Online (AJOL)

    The electrical resistivity of fresh Pre-Cambrian to Upper Cambrian crystalline basement rocks in southwestern Nigeria, hitherto inferred from sounding interpretation, has been determined from laboratory measurements. The rock types consist of granite gneiss, banded gneiss, augen gneiss, biotite granite, charnockite, ...

  4. First isolation of methicillin-resistant Staphylococcus aureus from pigs’ clinical samples in Serbia

    Directory of Open Access Journals (Sweden)

    Milenko Zutic

    2012-01-01

    Full Text Available Methicillin-resistant Staphylococcus aureus is a highly important human pathogen that is also a significant concern in veterinary medicine. Despite the high prevalence of colonization, clinical infections with methicillin-resistant Staphylococcus aureus appear to be rare in pigs. Methicillin-resistant Staphylococcus aureus was isolated from a sow with endometritis and her five piglets with dermatitis originating from a Serbian farm. Identification of the strains was done by automated system and confirmed by polymerase chain reaction for mecA and nuc genes. Detection of Staphylococcal Cassette Chromosome mec type was performed by multiplex polymerase chain reaction. Antimicrobial susceptibility testing on erythromycin, clindamycin, gentamicin, kanamycin, tobramycin, ciprofloxacin, tetracycline, trimethoprim/sulfamethoxazole and vancomycin was done by disc diffusion method. Six isolated strains from the infected sow and her piglets showed resistance only to tetracycline beside resistance to all beta-lactam antibiotics. In the tested methicillin-resistant Staphylococcus aureus isolates, Staphylococcal Cassette Chromosome mec type V was present. To our knowledge, this finding is the first documented detection of methicillin-resistant Staphylococcus aureus from pigs’ clinical samples in Serbia. The results of our study indicate the emergence of methicillin-resistant Staphylococcus aureus in a pig farm in Serbia highlighting the threat of this antibiotic-resistant microorganism as a pathogen causing both animal and human infections.

  5. Measuring Radionuclides in the environment: radiological quantities and sampling designs

    International Nuclear Information System (INIS)

    Voigt, G.

    1998-10-01

    One aim of the workshop was to support and provide an ICRU report committee (International Union of Radiation Units) with actual information on techniques, data and knowledge of modern radioecology when radionuclides are to be measured in the environment. It has been increasingly recognised that some studies in radioecology, especially those involving both field sampling and laboratory measurements, have not paid adequate attention to the problem of obtaining representative, unbiased samples. This can greatly affect the quality of scientific interpretation, and the ability to manage the environment. Further, as the discipline of radioecology has developed, it has seen a growth in the numbers of quantities and units used, some of which are ill-defined and which are non-standardised. (orig.)

  6. Ultra sound absorption measurements in rock samples at low temperatures

    Science.gov (United States)

    Herminghaus, C.; Berckhemer, H.

    1974-01-01

    A new technique, comparable with the reverberation method in room acoustics, is described. It allows Q-measurements at rock samples of arbitrary shape in the frequency range of 50 to 600 kHz in vacuum (.1 mtorr) and at low temperatures (+20 to -180 C). The method was developed in particular to investigate rock samples under lunar conditions. Ultrasound absorption has been measured at volcanics, breccia, gabbros, feldspar and quartz of different grain size and texture yielding the following results: evacuation raises Q mainly through lowering the humidity in the rock. In a dry compact rock, the effect of evacuation is small. With decreasing temperature, Q generally increases. Between +20 and -30 C, Q does not change much. With further decrease of temperature in many cases distinct anomalies appear, where Q becomes frequency dependent.

  7. New experimental procedure for measuring volume magnetostriction on powder samples

    International Nuclear Information System (INIS)

    Rivero, G.; Multigner, M.; Valdes, J.; Crespo, P.; Martinez, A.; Hernando, A.

    2005-01-01

    Conventional techniques used for volume magnetostriction measurements, as strain gauge or cantilever method, are very useful for ribbons or thin films but cannot be applied when the samples are in powder form. To overcome this problem a new experimental procedure has been developed. In this work, the experimental set-up is described, together with the results obtained in amorphous FeCuZr powders, which exhibit a strong dependence of the magnetization on the strength of the applied magnetic field. The magnetostriction measurements presented in this work point out that this dependence is related to a magnetovolume effect

  8. A random sample survey of initial drug resistance among tuberculosis cases in Latin America.

    Science.gov (United States)

    Laszlo, A.; de Kantor, I. N.

    1994-01-01

    A random sample survey of initial drug resistance among cases of tuberculosis in Latin America was carried out during the second half of the 1980s and the early 1990s. A total of 948 cultures of Mycobacterium tuberculosis isolated from patients presumed never before to have been treated for tuberculosis were collected from 30 randomly selected clusters in Latin America and tested for resistance to isoniazid, streptomycin, rifampicin, ethambutol, and thioacetazone. Initial drug resistance, although unevenly distributed, was detected in all the clusters tested and characterized one out of every six tuberculosis cases. Both single and multiple resistance to streptomycin and to isoniazid were the most prevalent forms throughout the region but were not sufficiently frequent to jeopardize significantly the outcome of short-course chemotherapy. However, localized pockets of high drug resistance occurred throughout the region and are cause for concern, especially in the case of rifampicin. PMID:7923539

  9. Evaluation of stress and saturation effects on seismic velocity and electrical resistivity - laboratory testing of rock samples

    Science.gov (United States)

    Vilhelm, Jan; Jirků, Jaroslav; Slavík, Lubomír; Bárta, Jaroslav

    2016-04-01

    Repository, located in a deep geological formation, is today considered the most suitable solution for disposal of spent nuclear fuel and high-level waste. The geological formations, in combination with an engineered barrier system, should ensure isolation of the waste from the environment for thousands of years. For long-term monitoring of such underground excavations special monitoring systems are developed. In our research we developed and tested monitoring system based on repeated ultrasonic time of flight measurement and electrical resistivity tomography (ERT). As a test site Bedřichov gallery in the northern Bohemia was selected. This underground gallery in granitic rock was excavated using Tunnel Boring Machine (TBM). The seismic high-frequency measurements are performed by pulse-transmission technique directly on the rock wall using one seismic source and three receivers in the distances of 1, 2 and 3 m. The ERT measurement is performed also on the rock wall using 48 electrodes. The spacing between electrodes is 20 centimeters. An analysis of relation of seismic velocity and electrical resistivity on water saturation and stress state of the granitic rock is necessary for the interpretation of both seismic monitoring and ERT. Laboratory seismic and resistivity measurements were performed. One series of experiments was based on uniaxial loading of dry and saturated granitic samples. The relation between stress state and ultrasonic wave velocities was tested separately for dry and saturated rock samples. Other experiments were focused on the relation between electrical resistivity of the rock sample and its saturation level. Rock samples with different porosities were tested. Acknowledgments: This work was partially supported by the Technology Agency of the Czech Republic, project No. TA 0302408

  10. Cultivation of vancomycin-resistant enterococci and methicillin-resistant staphylococci from input and output samples of German biogas plants.

    Science.gov (United States)

    Glaeser, Stefanie P; Sowinsky, Olivia; Brunner, Jana S; Dott, Wolfgang; Kämpfer, Peter

    2016-03-01

    Vancomycin-resistant enterococci (VRE) and methicillin-resistant staphylococci (MRS) were detected in two mesophilic German biogas plants (BGPs) using selective pre-enrichment methods combined with cultivation on CHROMagar media and antibiotic resistance gene screening. Genetic fingerprinting and 16S rRNA gene sequencing showed the presence of enterococci isolated by the VRE selective cultivation (67 isolates) in input and output samples of BGPs. In contrast, MRS (44 isolates) were detected in input, but in none of the output samples. Enterococcus isolates showed highest 16S rRNA gene sequence similarity (>99.8%) to E. lemanii, E. casseliflavus/E. gallinarium or E. devriesei/E. pseudoavium/E. viikkiensis and carried vanA, vanB and/or vanC1 genes. Enterococcus faecium and E. faecalis VRE were not detected, but isolates closely related to those species (>99.9% 16S rRNA gene sequence similarity) were detected by the MRS selective cultivation methods. Staphylococcus isolates shared highest 16S rRNA gene sequence similarity (>99.9%) with S. haemolyticus, S. lentus and S. sciuri and carried mecA genes. Methicillin-resistant S. aureus (MRSA) were not detected. In summary, manure from livestock husbandry contained both, VRE and MRS. VRE were also detected in output samples, indicating that enterococci with vancomycin resistance genes could be release into the environment by the application of BGP output material as biofertilizers. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Novel architecture for measurements in resistive MEMS sensors

    Science.gov (United States)

    Nandi, Prajit; Biswas, Riju; Sundar Dhar, Anindya; Das, Soumen

    2014-05-01

    A low voltage, low power, resistive sensor architecture is proposed in this paper. The architecture is novel as it enhances the sensitivity along the main axis as well as reducing the impact of cross axes components. The proposed scheme also allows the simultaneous measurement of sensitivity along six different axes. With less than 15% of the power of its Wheatstone bridge [1] counterpart and with a voltage level as low as 2.25 V, this architecture also enables the realization of the sensor using fewer resistive elements. The modified sensor structure, along with the front-end signal processing circuit, is discussed.

  12. Leaching of Conductive Species: Implications to Measurements of Electrical Resistivity.

    Science.gov (United States)

    Spragg, R; Jones, S; Bu, Y; Lu, Y; Bentz, D; Snyder, K; Weiss, J

    2017-05-01

    Electrical tests have been used to characterize the microstructure of porous materials, the measured electrical response being determined by the contribution of the microstructure (porosity and tortuosity) and the electrical properties of the solution (conductivity of the pore solution) inside the pores of the material. This study has shown how differences in concentration between the pore solution (i.e., the solution in the pores) and the storage solution surrounding the test specimen leads to significant transport (leaching) of the conductive ionic species between the pore solution and the storage solution. Leaching influences the resistivity of the pore solution, thereby influencing electrical measurements on the bulk material from either a surface or uniaxial bulk resistance test. This paper has three main conclusions: 1.) Leaching of conductive species does occur with concentration gradients and that a diffusion based approach can be used to estimate the time scale associated with this change. 2.) Leaching of ions in the pore solution can influence resistivity measurements, and the ratio of surface to uniaxial resistivity can be used as a method to assess the presence of leaching and 3.) An estimation of the magnitude of leaching for standardized tests of cementitious materials.

  13. Aerial sampling using drones for measuring trace gases

    Science.gov (United States)

    Chang, Chih-Chung; Wang, Jia-Lin; Chang, Chih-Yuan; Lin, Ming-Ren; Ou-Yang, Chang-Feng; Pan, Xiang-Xu; Lin, Neng-Huei

    2017-04-01

    Aerial and ground-level samples were simultaneously collected at the northern tip of Taiwan, Cape Fuguei, which is commonly served as a receptor site to receive air parcels from East Asia, Asian continent, the northwest Pacific Ocean and the island of Taiwan itself. Both aerial and surface samples were analyzed for 106 volatile organic compounds (VOCs) and divided into three categories as follows: 1. the total concentrations of 106 VOC (T-VOC) at 300-m height that are lower than the T-VOC level at the surface (Category A), 2. T-VOC concentrations at 300-m that are higher than those at the surface (Category B), and 3. comparable concentrations (Category C). Ten VOCs were exploited as tracers for a variety of emission sources linking to possible sources and transport routes of airborne pollutants. The first two categories A and B showed significant differences in the observed composition and concentrations of tracers between aerial and ground-level samples, implying that the pollutants at different heights may have resulted from different sources and poor air mixing, despite only a 300-m difference in vertical height. In contrast, Category C showed good vertical mixing, as indicated by the comparable concentrations between the aerial and surface measurements. Since the three categories occurred in specific meteorological conditions (between, prior to, and after cold fronts), respectively, it suggests that varied prevailing meteorology controlled the distribution and transport of airborne pollutants. Unlike sampling commonly performed at the surface, this study uses aerial sampling to demonstrate that layered structures under different meteorological conditions. Sampling aloft in lower boundary layer avoids samples being over-influenced by the close-by surface sources such as traffic to reveal signatures of a broader region.

  14. Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples.

    Science.gov (United States)

    Lautenbach, Ebbing; Santana, Evelyn; Lee, Abby; Tolomeo, Pam; Black, Nicole; Babson, Andrew; Perencevich, Eli N; Harris, Anthony D; Smith, Catherine A; Maslow, Joel

    2008-04-01

    We assessed the rate of recovery of fluoroquinolone-resistant and fluoroquinolone-susceptible Escherichia coli isolates from culture of frozen perirectal swab samples compared with the results for culture of the same specimen before freezing. Recovery rates for these 2 classes of E. coli were 91% and 83%, respectively. The majority of distinct strains recovered from the initial sample were also recovered from the frozen sample. The strains that were not recovered were typically present only in low numbers in the initial sample. These findings emphasize the utility of frozen surveillance samples.

  15. Transport measurements in superconductors: critical current of granular high TC ceramic superconductor samples

    International Nuclear Information System (INIS)

    Passos, W.A.C.

    2016-01-01

    This work presents a method to obtain critical current of granular superconductors. We have carried out transport measurements (ρxT curves and VxI curves) in a YBa 2 Cu 3 O 7-δ sample to determine critical current density of it. Some specimens reveal a 'semiconductor-like' behavior (electrical resistivity decreases with increasing temperatures above critical temperature T c of material) competing with superconductor behavior. Due to high granular fraction of the sample, these competition is clearly noted in ρxT curves. Measurements carried out from 0 to 8500 Oe of applied field show the same behavior, and the critical current density of the samples is shown. (author)

  16. Standard test method for conducting potentiodynamic polarization resistance measurements

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    1997-01-01

    1.1 This test method covers an experimental procedure for polarization resistance measurements which can be used for the calibration of equipment and verification of experimental technique. The test method can provide reproducible corrosion potentials and potentiodynamic polarization resistance measurements. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

  17. Arrester Resistive Current Measuring System Based on Heterogeneous Network

    Science.gov (United States)

    Zhang, Yun Hua; Li, Zai Lin; Yuan, Feng; Hou Pan, Feng; Guo, Zhan Nan; Han, Yue

    2018-03-01

    Metal Oxide Arrester (MOA) suffers from aging and poor insulation due to long-term impulse voltage and environmental impact, and the value and variation tendency of resistive current can reflect the health conditions of MOA. The common wired MOA detection need to use long cables, which is complicated to operate, and that wireless measurement methods are facing the problems of poor data synchronization and instability. Therefore a novel synchronous measurement system of arrester current resistive based on heterogeneous network is proposed, which simplifies the calculation process and improves synchronization, accuracy and stability and of the measuring system. This system combines LoRa wireless network, high speed wireless personal area network and the process layer communication, and realizes the detection of arrester working condition. Field test data shows that the system has the characteristics of high accuracy, strong anti-interference ability and good synchronization, which plays an important role in ensuring the stable operation of the power grid.

  18. THE EXPERIMENTAL VALIDATION OF THE GROUNDING DEVICE RESISTANCE MEASUREMENT METHOD

    Directory of Open Access Journals (Sweden)

    I. V. Nizhevskyi

    2016-12-01

    Full Text Available Purpose. The paper considers experimental research of three-electrode units for measuring the resistance of grounding devices for different purposes. Methodology. The experimental study of the method of resistance measurement of grounding devices for any design in any soil structure using the method of physical modeling is presented. Results. By results of model operation the set of equations of the sixth order is solved. It allowed to determine the own and mutual impedance in the three-electrode unit with high accuracy without searching the point of zero potential. Features of measuring and defining the own and relative resistances of various combinations of electrodes for three-electrode measuring unit are considered. Originality. The necessity of finding a zero potential point is excluded. Practical value. The proposed method provides the smallest possible spacing of potential electrodes outside the grounding devices. This reduces the wiring length measurement circuit in several times, increases the ratio «signal – noise», removes the restrictions on building of the territory outside the test grounding device.

  19. Characterization of novel antibiotic resistance genes identified by functional metagenomics on soil samples.

    Science.gov (United States)

    Torres-Cortés, Gloria; Millán, Vicenta; Ramírez-Saad, Hugo C; Nisa-Martínez, Rafael; Toro, Nicolás; Martínez-Abarca, Francisco

    2011-04-01

    The soil microbial community is highly complex and contains a high density of antibiotic-producing bacteria, making it a likely source of diverse antibiotic resistance determinants. We used functional metagenomics to search for antibiotic resistance genes in libraries generated from three different soil samples, containing 3.6 Gb of DNA in total. We identified 11 new antibiotic resistance genes: 3 conferring resistance to ampicillin, 2 to gentamicin, 2 to chloramphenicol and 4 to trimethoprim. One of the clones identified was a new trimethoprim resistance gene encoding a 26.8 kDa protein closely resembling unassigned reductases of the dihydrofolate reductase group. This protein, Tm8-3, conferred trimethoprim resistance in Escherichia coli and Sinorhizobium meliloti (γ- and α-proteobacteria respectively). We demonstrated that this gene encoded an enzyme with dihydrofolate reductase activity, with kinetic constants similar to other type I and II dihydrofolate reductases (K(m) of 8.9 µM for NADPH and 3.7 µM for dihydrofolate and IC(50) of 20 µM for trimethoprim). This is the first description of a new type of reductase conferring resistance to trimethoprim. Our results indicate that soil bacteria display a high level of genetic diversity and are a reservoir of antibiotic resistance genes, supporting the use of this approach for the discovery of novel enzymes with unexpected activities unpredictable from their amino acid sequences. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

  20. Epidemiologic evaluation of Vancomycin Resistant genes in Enterococcus spp. isolated from clinical samples

    Directory of Open Access Journals (Sweden)

    Omid Teymournejad

    2011-09-01

    Full Text Available Background & Objectives: Isolation of vancomycin resistant Enterococcus from clinical samples is very important. The aim of this study was evaluation of phenotype and genotype of van genes in vancomycine resistant Enterococcus. Materials and Methods: 411 Enterococcus isolates were collected from selected Tehran’s hospitals between March 2004 and December 2007. The enterococcal isolates were identified by biochemical confirmation tests. Resistance of each isolate to vancomycin determined by disk diffusion and agar dilution test. The presence of the vanA, B, C, D, E resistance gene was assessed by PCR. Results: 185(45% and 23(5.6% with disc-diffusion method and agar-dilution method were resistant to vancomucin (VRE and all of VREs were Enterococcus faecium. 12 (52.2%, 7(30.4% of the VRE isolates had vanA, vanB and 3(13% had both of vanA and vanB gene. Conclusion: Most important mechanism for high level resistance to vancomycin is presence of van genes and these genes can transfer between Enterococci. Significance of investigation in molecular level of resistance to vancomycin was due to relation between phenotypic resistant and presence of van genes.

  1. Evaluation of pavement skid resistance using high speed texture measurement

    Directory of Open Access Journals (Sweden)

    Jay N. Meegoda

    2015-12-01

    Full Text Available Skid resistance is an important parameter for highway designs, construction, management, maintenance and safety. The purpose of this manuscript is to propose the correlation between skid resistance, which is measured as skid resistance trailer, and mean profile depth (MPD or the macro surface texture, which is measured by vehicle mounted laser, so that highway agencies can predict the skid resistance of pavement without the use of expensive and time consuming skid resistance trailer, which also causes disruption of traffic in use. In this research skid numbers and MPD from 5 new asphalt pavements and 4 old asphalt pavements were collected using a locked wheel skid trailer and a vehicle mounted laser. Using the data collected, a correlation between the skid number (SN40R collected by locked wheel skid tester and the texture data or MPD collected by a vehicle mounted laser operating at highway speeds was developed. The proposed correlation for new pavements was positive for MPD values less than 0.75 mm to reach a peak SN40R value, then there was a negative correlation as the MPD increases until the MPD value was equal to 1.1 mm and beyond the MPD value of 1.1 mm to the maximum value of 1.4 mm, SN40R value remained almost constant. There were significant data scatter for the MPD value of 0.8 mm. To explain these results, water film thickness during the friction test was calculated and the critical MPD was defined. The effect of sealed water pool on the SN40R was discussed. The test result showed a similar trend for older asphalt pavements, but with lower SN40R values due to the polishing of pavement micro-texture by traffic. Hence, a reduction factor was proposed for older pavements based on cumulative traffic volume for the above correlation to predict the skid resistance of older pavements.

  2. Antibiotic Resistance Among Staphylococcus aureus and Escherichia coli Isolated From Traditional and Industrial Food Samples

    Directory of Open Access Journals (Sweden)

    Mojtaba Arslani

    2017-05-01

    Full Text Available Background: Foodborne diseases are one of the serious problems in the world. Every year, more than 100 million people are affected by foodborne and waterborne diseases particularly immunocompromised diseases. Objectives: The aim of the present study was to evaluate bacterial load and antibiotic resistance pattern in bacterial isolates from food samples of meat, dairy, and pastry products from west of Tehran, Iran, during April 2007 to March 2008. Materials and Methods: A total of 1625 different food samples including dairy products, meat and pastries were collected randomly from different parts of the west of Tehran. All samples were kept at 4°C. The samples were first cultured according to the standard bacteriological methods and then Staphylococcus aureus and Escherichia coli isolates were identified using standard bacteriological tests. Antimicrobial susceptibility test was performed by disk diffusion method according to Clinical & Laboratory Standards Institute (CLSI guidelines. Results: During 2007 and 2008, 2.8% and 3% of the food samples were contaminated with S. aureus. Similarly, 3.5% and 6.4% of the food samples were contaminated with E. coli. E. coli isolates were highly resistant to amikacin and cephotaxime and this resistance was increased in 2008. Similarly S. aureus isolates were resistant to ciprofloxacin, cephotaxime, gentamicin, and tetracyclin. There was no significant difference during 2007-2008. Conclusion: The rate of contamination during 2007 was 2.8% and during 2008 was 3% for S. aureus. This strain was isolated from the food samples. Further studies should be done to determine the changes of bacterial resistance pattern for various food samples. Thus, the baseline for comparison with future prospective studies should be established, enabling the determination of trends over time.

  3. Lattice strain measurement in rock sample by neutron diffraction technique

    International Nuclear Information System (INIS)

    Abe, Jun; Ito, Takayoshi; Sekine, Kotaro; Harjo, Stefanus; Aizawa, Kazuya

    2015-01-01

    Strain gauge and mechanical extensometers are commonly used to measure strain in rock samples. In recent years, diffraction techniques with X-rays and neutrons for investigating strain in engineering materials have been developed. Strain measurements using diffraction technique are based on Bragg's law. Lattice spacing changes with strain, which induces peak shift of Bragg peak. Strain value can be estimated from this peak shift value. Strain measurements using the world's highest intensity neutron beam can be performed at Japan Proton Accelerator Research Complex (J-PARC) constructed at Tokai in Ibaraki. Neutron powder diffractometer dedicating to investigate strain state in engineering materials has been constructed at BL19 in J-PARC, which is named as The Engineering Materials Diffractometer 'TAKUMI'. In order to examine applicability of the diffractometer to rock materials, in situ neutron diffraction experiments on rock samples under uni-axial compression have been performed. Higher resolution strain data has been obtained in shorter time compared to other diffractometers. In addition, neutron diffraction peaks of not only major component, such as quartz, but also minor components, such as feldspars, could be observed. Anisotropy of strain with respect to the quartz crystal orientation and discrepancy between macroscopic strain (measured by strain gauge) and lattice strain (measured by neutron diffraction) were also recognized. Change in peak width with respect to stress magnitude showed a different behavior depending on rock type. Strain measurements using neutron diffraction technique give us new insight in rock deformation which cannot be obtained by common technique. (author)

  4. Frequency, Antimicrobial Resistance and Genetic Diversity of Klebsiella pneumoniae in Food Samples.

    Directory of Open Access Journals (Sweden)

    Yumei Guo

    Full Text Available This study aimed to assess the frequency of Klebsiella pneumoniae in food samples and to detect antibiotic resistance phenotypes, antimicrobial resistance genes and the molecular subtypes of the recovered isolates. A total of 998 food samples were collected, and 99 (9.9% K. pneumoniae strains were isolated; the frequencies were 8.2% (4/49 in fresh raw seafood, 13.8% (26/188 in fresh raw chicken, 11.4% (34/297 in frozen raw food and 7.5% (35/464 in cooked food samples. Antimicrobial resistance was observed against 16 antimicrobials. The highest resistance rate was observed for ampicillin (92.3%, followed by tetracycline (31.3%, trimethoprim-sulfamethoxazole (18.2%, and chloramphenicol (10.1%. Two K. pneumoniae strains were identified as extended-spectrum β-lactamase (ESBL-one strain had three beta-lactamases genes (blaSHV, blaCTX-M-1, and blaCTX-M-10 and one had only the blaSHV gene. Nineteen multidrug-resistant (MDR strains were detected; the percentage of MDR strains in fresh raw chicken samples was significantly higher than in other sample types (P<0.05. Six of the 18 trimethoprim-sulfamethoxazole-resistant strains carried the folate pathway inhibitor gene (dhfr. Four isolates were screened by PCR for quinolone resistance genes; aac(6'-Ib-cr, qnrB, qnrA and qnrS were detected. In addition, gyrA gene mutations such as T247A (Ser83Ile, C248T (Ser83Phe, and A260C (Asp87Ala and a parC C240T (Ser80Ile mutation were identified. Five isolates were screened for aminoglycosides resistance genes; aacA4, aacC2, and aadA1 were detected. Pulsed-field gel electrophoresis-based subtyping identified 91 different patterns. Our results indicate that food, especially fresh raw chicken, is a reservoir of antimicrobial-resistant K. pneumoniae, and the potential health risks posed by such strains should not be underestimated. Our results demonstrated high prevalence, antibiotic resistance rate and genetic diversity of K. pneumoniae in food in China. Improved

  5. Sampling and Pooling Methods for Capturing Herd Level Antibiotic Resistance in Swine Feces using qPCR and CFU Approaches.

    Science.gov (United States)

    Schmidt, Gunilla Veslemøy; Mellerup, Anders; Christiansen, Lasse Engbo; Ståhl, Marie; Olsen, John Elmerdahl; Angen, Øystein

    2015-01-01

    The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic resistance genes in total DNA from swine fecal samples that were obtained using different sampling and pooling methods. In parallel, the number of antibiotic resistant coliform indicator bacteria was determined in the same swine fecal samples. The results showed that the qPCR assays were capable of detecting differences in antibiotic resistance levels in individual animals that the coliform bacteria colony forming units (CFU) could not. Also, the qPCR assays more accurately quantified antibiotic resistance genes when comparing individual sampling and pooling methods. qPCR on pooled samples was found to be a good representative for the general resistance level in a pig herd compared to the coliform CFU counts. It had significantly reduced relative standard deviations compared to coliform CFU counts in the same samples, and therefore differences in antibiotic resistance levels between samples were more readily detected. To our knowledge, this is the first study to describe sampling and pooling methods for qPCR quantification of antibiotic resistance genes in total DNA extracted from swine feces.

  6. Standardizing measurement, sampling and reporting for public exposure assessments

    Energy Technology Data Exchange (ETDEWEB)

    Rochedo, Elaine R.R. [Instituto de Radioprotecao e Dosimetria, Comissao Nacional de Energia Nuclear, Av. Salvador Allende s/No. CEP 22780-160 Rio de Janeiro, RJ (Brazil)], E-mail: elaine@ird.gov.br

    2008-11-15

    UNSCEAR assesses worldwide public exposure from natural and man-made sources of ionizing radiation based on information submitted to UNSCEAR by United Nations Member States and from peer reviewed scientific literature. These assessments are used as a basis for radiation protection programs of international and national regulatory and research organizations. Although UNSCEAR describes its assessment methodologies, the data are based on various monitoring approaches. In order to reduce uncertainties and improve confidence in public exposure assessments, it would be necessary to harmonize the methodologies used for sampling, measuring and reporting of environmental results.

  7. Radioactivity measurements of soil samples from Raichur of Karnataka, India

    International Nuclear Information System (INIS)

    Rajesh, S.; Kerur, B.R.; Anilkumar, S.

    2016-01-01

    Radiations present in environment comprise the terrestrial and extraterrestrial radiations. Natural radioactivity measurement, radiation monitoring of the region, dose assessment and interpretation of radiological related parameters are crucial aspects from the public awareness and environmental safety point of view. It is found that values of the radiological parameters obtained for samples in the study area were found within acceptable or permissible limits. The estimated gamma absorbed dose rate was found to be in the range 32 - 162 nGy h -1 . Higher dose rate than that of the normal dose rate were observed in the regions where granitic, granitic gneiss and schist outcrops were prominent area

  8. Nonlinearity of resistive impurity effects on van der Pauw measurements

    Science.gov (United States)

    Koon, D. W.

    2006-09-01

    The dependence of van der Pauw resistivity measurements on local macroscopic inhomogeneities is shown to be nonlinear. A resistor grid network models a square laminar specimen, enabling the investigation of both positive and negative local perturbations in resistivity. The effect of inhomogeneity is measured both experimentally, for an 11×11 grid, and computationally, for both 11×11 and 101×101 grids. The maximum "shortlike" perturbation produces 3.1±0.2 times the effect predicted by the linear approximation, regardless of its position within the specimen, while all "openlike" perturbations produce a smaller effect than predicted. An empirical nonlinear correction for f(x ,y) is presented which provides excellent fit over the entire range of both positive and negative perturbations for the entire specimen.

  9. Chemical Composition Measurements of LAWA44 Glass Samples

    Energy Technology Data Exchange (ETDEWEB)

    Fox, K. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Edwards, T. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Riley, W. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-11-15

    DOE is building the Hanford Tank Waste Treatment and Immobilization Plant (WTP) at the Hanford Site in Washington to remediate 55 million gallons of radioactive waste that is temporarily stored in 177 underground tanks. Both low-activity and high-level wastes will then be vitrified into borosilicate glass using Joule-heated ceramic melters. Efforts are being made to increase the loading of Hanford tank wastes in the glass. One area of work is enhancing waste glass composition/property models and broadening the compositional regions over which those models are applicable. In this report, the Savannah River National Laboratory provides chemical analysis results for several samples of a simulated low-activity waste glass, LAWA44, provided by the Pacific Northwest National Laboratory as part of an ongoing development task. The measured chemical composition data are reported and compared with the targeted values for each component for each glass. A detailed review showed no indications of errors in the preparation or measurement of the study glasses. All of the measured sums of oxides for the study glasses fell within the interval of 97.9 to 102.6 wt %, indicating acceptable recovery of the glass components. Comparisons of the targeted and measured chemical compositions showed that the measured values for the glasses met the targeted concentrations within 10% for those components present at more than 5 wt %. It was noted that the measured B2O3 concentrations are somewhat above the targeted values for the study glasses. No obvious trends were observed with regard to the multiple melting steps used to prepare the study glasses, indicating that any potential effects of volatility were below measurable thresholds.

  10. The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches.

    Science.gov (United States)

    Andersen, V D; DE Knegt, L V; Munk, P; Jensen, M S; Agersø, Y; Aarestrup, F M; Vigre, H

    2017-10-01

    The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.

  11. First Record of Isolation and Characterization of Methicillin Resistant Staphylococcus lugdunensis from Clinical Samples in Iraq

    Science.gov (United States)

    Al-Charrakh, Alaa H.; Obayes, Mohammed H.

    2014-01-01

    This study was conducted to determine the frequency of Staphylococcus lugdunensis in different clinical samples. Out of 690 clinical samples, a total of 178 coagulase negative staphylococci (CoNS) isolates were recovered. CoNS were identified as 10 different species; 22 isolates belonged to Staphylococcus lugdunensis. Two specific genes for S. lugdunensis were used ( tanA gene and fbl gene) to confirm identification. Both of these specific genes were detected in 15 (68.1%) of 22 isolates that were identified phenotypically. The results of oxacillin MIC showed that 7 of the 15 (46.6%) S. lugdunensis isolates were oxacillin resistant. The antibiotic susceptibility testing against 16 antibiotics showed that resistance rates were variable towards these antibiotics. Eight of fifteen S. lugdunensis isolates (53.3%) were β-lactamase producer. Results of molecular detection of mecA gene found that mecA gene was detected in 6 (40%) of 15 S. lugdunensis. All of these 6 isolates (S1, S2, S3, S4, S5, and S6) were resistant to oxacillin. One isolate (S7) was resistant to oxacillin but mecA was not detected in this isolate. This study is a first record of isolation and characterization of methicillin resistant S. lugdunensis (MRSL) from clinical samples in Iraq. PMID:25126573

  12. Noncontact true temperature measurement. [of levitated sample using laser pyrometer

    Science.gov (United States)

    Lee, Mark C.; Allen, James L.

    1987-01-01

    A laser pyrometer has been developed for acquiring the true temperature of a levitated sample. The laser beam is first expanded to cover the entire cross-sectional surface of the target. For calibration of such a system, the reflectivity signal of an ideal 0.95 cm diameter gold-coated sphere (reflectivity = 0.99) is used as the reference for any other real targets. The emissivity of the real target can then be calculated. The overall system constant is obtained by passively measuring the radiance of a blackbody furnace (emissivity = 1.0) at a known, arbitrary temperature. Since the photo sensor used is highly linear over the entire operating temperature range, the true temperature of the target can then be computed. Preliminary results indicate that true temperatures thus obtained are in excellent correlation with thermocouple measured temperatures.

  13. Novel geometry for simultaneous resistive, Hall and optical measurement of MgHx thin films

    Science.gov (United States)

    Koon, D. W.; Griffin, C. C. W.; Ares, J. R.; Leardini, F.; Sanchez, C.

    2009-03-01

    We describe a novel specimen geometry we have used to simultaneously probe optical transmission, sheet resistance and sheet Hall resistance in 100nm Mg films during hydrogen absorption. A Mg-film cloverleaf overlaps four rectangular Pd pads at the corners of a glass slide, a variation on a two-pad geometry used by Ingason and Olafsson for resistive studies of Pd-capped MgHx films [J. Alloys and Compounds 404- 406 (2005), 469-72.]. Hydrogen diffuses laterally through the Pd pads before entering the magnesium layer from below. The sample holder also includes an LED-photodiode pair for measuring [monochromatic] optical transmission. We show that the simultaneous measurement of these three quantities during the metal-to-insulator transition in a hydriding MgHx film allows for a more complete understanding of the hydriding process in these films.

  14. Sampling and Pooling Methods for Capturing Herd Level Antibiotic Resistance in Swine Feces using qPCR and CFU Approaches

    DEFF Research Database (Denmark)

    Schmidt, Gunilla Veslemøy; Mellerup, Anders; Christiansen, Lasse Engbo

    2015-01-01

    The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays...... for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic resistance genes in total DNA from swine fecal samples that were obtained using different sampling and pooling methods. In parallel, the number of antibiotic resistant coliform indicator bacteria was determined...... in the same swine fecal samples. The results showed that the qPCR assays were capable of detecting differences in antibiotic resistance levels in individual animals that the coliform bacteria colony forming units (CFU) could not. Also, the qPCR assays more accurately quantified antibiotic resistance genes...

  15. High mechanical Q-factor measurements on silicon bulk samples

    Energy Technology Data Exchange (ETDEWEB)

    Nawrodt, R; Zimmer, A; Koettig, T; Schwarz, C; Heinert, D; Hudl, M; Neubert, R; Thuerk, M; Nietzsche, S; Vodel, W; Seidel, P [Friedrich-Schiller-Universitaet, Institut fuer Festkoerperphysik, Helmholtzweg 5, D-07743 Jena (Germany); Tuennermann, A [Friedrich-Schiller-Universitaet, Institut fuer Angewandte Physik, Max-Wien-Platz 1, D-07743 Jena (Germany)], E-mail: ronny.nawrodt@uni-jena.de

    2008-07-15

    Future gravitational wave detectors will be limited by different kinds of noise. Thermal noise from the coatings and the substrate material will be a serious noise contribution within the detection band of these detectors. Cooling and the use of a high mechanical Q-factor material as a substrate material will reduce the thermal noise contribution from the substrates. Silicon is one of the most interesting materials for a third generation cryogenic detector. Due to the fact that the coefficient of thermal expansion vanishes at 18 and 125 K the thermoelastic contribution to the thermal noise will disappear. We present a systematic analysis of the mechanical Q-factor at low temperatures between 5 and 300 K on bulk silicon (100) samples which are boron doped. The thickness of the cylindrical samples is varied between 6, 12, 24, and 75mm with a constant diameter of 3 inches. For the 75mm substrate a comparison between the (100) and the (111) orientation is presented. In order to obtain the mechanical Q-factor a ring-down measurement is performed. Thus, the substrate is excited to resonant vibrations by means of an electrostatic driving plate and the subsequent ring-down is recorded using a Michelson-like interferometer. The substrate itself is suspended as a pendulum by means of a tungsten wire loop. All measurements are carried out in a special cryostat which provides a temperature stability of better than 0.1K between 5 and 300K during the experiment. The influence of the suspension on the measurements is experimentally investigated and discussed. At 5.8K a highest Q-factor of 4.5 x 10{sup 8} was achieved for the 14.9 kHz mode of a silicon (100) substrate with a diameter of 3 inches and a thickness of 12 mm.

  16. Residual Resistivity Ratio (RRR) Measurements of LHC Superconducting NbTi Cable Strands

    CERN Document Server

    Charifoulline, Z

    2006-01-01

    The Rutherford-type superconducting NbTi cables of the LHC accelerator are currently manufactured by six industrial companies. As a part of the acceptance tests, the Residual Resistivity Ratio (RRR) of superconducting strands is systematically measured on virgin strands to qualify the strands before cabling and on extracted strands to qualify the cables and to check the final heat treatment (controlled oxidation to control interstrand resistance). More than 12000 samples of virgin and extracted strands have been measured during last five years. Results show good correlation with the measurements done by the companies and reflect well the technological process of cable production (strand annealing, cabling, cable heat treatment). This paper presents a description of the RRR-test station and the measurement procedure, the summary of the results over all suppliers and finally the correlation between RRR-values of the cables and the magnets.

  17. Development of Antibiotic Resistance Against Ureaplasma urealyticum Strains Isolated from Urogenital Samples

    Directory of Open Access Journals (Sweden)

    Musa Saraçoğlu

    2018-04-01

    Full Text Available Objective: To assess any change in the antibiotic sensitivity of Ureaplasma urealyticum strains isolated from urogenital samples in the course of time. Materials and Methods: Hospital records were retrospectively examined and cases with growth of U. urealyticum in urogenital samples in the years 2008 and 2013 were identified. Furthermore, the change in the course of time was examined by taking into consideration the cases we reported in 2001. Results: Higher rates of sensitivity against tetracycline and doxycycline were observed in 60 patients with isolated U. urealyticum. Higher rates of resistance against ofloxacin and ciprofloxacin were observed. A significant difference was found in resistance against antibiotics when the records of 2008 and 2013 were compared. A statistically significant increase was found in resistance against ofloxacin and ciprofloxacin when the records of 2001 were compared with the records of 2008 and 2013 (p<0.0005. Conclusion: U. urealyticum strains demonstrated high levels of resistance to quinolones. Resistance development is increasing in the course of time. Sensitivity against tetracycline and doxycycline has continued at high rates. It would be beneficial to consider these results during empirical treatment to be applied in cases ineligible for culturing.

  18. Usefulness of rapid urease test samples for molecular analysis of clarithromycin resistance in Helicobacter pylori.

    Science.gov (United States)

    Baroni, María R; Bucci, Pamela; Giani, Rita N; Giusti, Antonela; Tedeschi, Fabian A; Salvatierra, Emiliano; Barbaglia, Yanina; Jimenez, Félix; Zalazar, Fabian E

    2018-03-27

    Helicobacter pylori is a gastric pathogen that is widely recognized as a causative agent of gastric disease. Its eradication is variable, mainly due to increased resistance to clarithromycin. Our objective was: to evaluate (i) if the biopsy specimen used for the rapid urease test is a useful sample to detect resistance to clarithromycin by PCR-RFLP and (ii) the distribution of A2142G and A2143G point mutations in the 23S rRNA gene, in relation to virulence factors in our region. Gastric specimens were collected from adult dyspeptic patients (n=141) and H. pylori was investigated by the rapid urease test, histopathological analysis and PCR for the hsp60 gene. Clarithromycin resistance was detected by PCR-RFLP in 62 H. pylori (+) paired biopsy specimens submitted to molecular analysis and the rapid urease test. H. pylori virulence factors were analyzed by multiplex PCR using specific primers for the cagA, vacA and babA2 genes. Thirteen out of 62 strains (20.9%) were resistant to clarithromycin: 6/13 (46.2%) harbored the A2143G mutation whereas 7/13 (53.8%) carried the A2142G point mutation. vacA m1s1 was the most frequent genotype among the resistant strains. In conclusion, the biopsy specimens used for the rapid urease test were suitable samples for clarithromycin resistance detection in patients infected with H. pylori, which became especially useful in cases where the number or size of the biopsies is limited. In addition, this is the first report of a molecular analysis for clarithromycin resistance performed directly from gastric biopsies in our region. Copyright © 2018 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  19. Methodic aspects of autoclave tests of compact samples on cyclic crack resistance

    International Nuclear Information System (INIS)

    Pokhmurskij, V.I.; Gnyp, I.P.; Popov, A.A.; Tarasyuk, G.P.; Dutsyak, I.Z.; Timonin, V.M.

    1984-01-01

    Laboratory autoclave LAKIM-25 was created for investigation of cyclic and static crack resistance of vessel materials of power plants under the conditions close to operation ones. It enables to investigate the cyclic crack resistance of standard samples of up to 25 mm thickness in water at 623 K and 18 MPa. The friction force changes during tests from 6.1 up to 6.4 kN, therefore it is necessary to correct regularly the force of sample loading, recording the s-n diagram on a drum of UMEh-10TM plant. The periodic record of loading diagram coincides with respect to time with the necessity of controlling the change of sample pliability during crack propagation

  20. A new site for 85Kr measurements on groundwater samples

    International Nuclear Information System (INIS)

    Lange, T.; Hebert, D.

    2001-01-01

    As a part of a new 85 Kr laboratory, which is currently being established at the Institute of Applied Physics in Freiberg, Germany, a modified CO 2 extractor for krypton sampling is used. The operation principle is simple and contamination-safe with a reasonable effort. Continuously pumped under pressure, the water passes a Venturi-type nozzle and degasses due to relaxing. The extracted gas mixture then enters a recirculation system flowing through a CO 2 trap (NaOH), molecular sieves and a cooled charcoal trap, where krypton and other components are adsorbed. Remaining gases reenter the system at the Venturi-type nozzle. To keep the circulation alive an additional helium support is needed. In a simple field experiment, extraction efficiencies up to 0.8 for 222 Rn have been measured.

  1. Measuring Resistive Wall Mode Stability in Real-time

    Science.gov (United States)

    Hanson, J. M.; Lanctot, M. J.; Navratil, G. A.; Reimerdes, H.; Strait, E. J.

    2009-11-01

    Measurements of the plasma response to externally applied, low-n magnetic fields can be used to determine the resistive wall mode (RWM) stability of the plasma equilibrium. Such a method, if implemented as a real-time algorithm, can be used to gate error field correction, profile control, and RWM feedback control algorithms, enabling operation close to the no-wall stability limit. In addition, the stability estimate can be used to directly update parameters in an advanced RWM controller as the plasma evolves. We have developed an efficient scheme that uses an external field rotating at a single fixed frequency. Because only one frequency is applied, the plasma response can be calculated from measurements by Fourier-analyzing the measurements at only the applied frequency and subtracting the known vacuum pickup due to the control coils. This single-frequency, Fourier-domain analysis uses a small number of arithmetical operations, which is a requisite for real-time implementation.

  2. Sensitivity of resistive and Hall measurements to local inhomogeneities

    Science.gov (United States)

    Koon, Daniel W.; Wang, Fei; Hjorth Petersen, Dirch; Hansen, Ole

    2013-10-01

    We derive exact, analytic expressions for the sensitivity of resistive and Hall measurements to local inhomogeneities in a specimen's material properties in the combined linear limit of a weak perturbation over an infinitesimal area in a small magnetic field. We apply these expressions both to four-point probe measurements on an infinite plane and to symmetric, circular van der Pauw discs, obtaining functions consistent with published results. These new expressions speed up calculation of the sensitivity for a specimen of arbitrary shape to little more than the solution of two Laplace equation boundary-value problems of the order of N3 calculations, rather than N2 problems of total order N5, and in a few cases produces an analytic expression for the sensitivity. These functions provide an intuitive, visual explanation of how, for example, measurements can predict the wrong carrier type in n-type ZnO.

  3. Sensitivity of resistive and Hall measurements to local inhomogeneities

    DEFF Research Database (Denmark)

    Koon, Daniel W.; Wang, Fei; Petersen, Dirch Hjorth

    2013-01-01

    We derive exact, analytic expressions for the sensitivity of resistive and Hall measurements to local inhomogeneities in a specimen's material properties in the combined linear limit of a weak perturbation over an infinitesimal area in a small magnetic field. We apply these expressions both to four-point...... probe measurements on an infinite plane and to symmetric, circular van der Pauw discs, obtaining functions consistent with published results. These new expressions speed up calculation of the sensitivity for a specimen of arbitrary shape to little more than the solution of two Laplace equation boundary......-value problems of the order of N3 calculations, rather than N2 problems of total order N5, and in a few cases produces an analytic expression for the sensitivity. These functions provide an intuitive, visual explanation of how, for example, measurements can predict the wrong carrier type in n-type ZnO....

  4. Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples

    Directory of Open Access Journals (Sweden)

    Mehmet ELMALI

    Full Text Available Abstract The objectives of this study were i to isolate Arcobacter species (Arcobacter butzleri, Arcobacter skirrowii, Arcobacter cryaerophilus from different foods and sources, ii to verify the isolates by multiplex PCR assay, iii to detect the antibiotic resistance profiles of the isolates. In this study a total of 60 Arcobacter isolates were obtained. Arcobacter species were mostly isolated from swab samples (40%, followed by wastewater (29.1%, broiler wing meat (30%, raw milk (23.9% and minced meat (6.6%. Regarding the seasonal distribution of Arcobacter from swab and wastewater samples, the bacterium was commonly isolated from wastewater in winter and spring, while it was frequently detected in swab samples during autumn and spring. All of the isolates were found to be resistant to nalidixic acid, ampicillin, rifampin, and erythromycin. The most effective antibiotic was tetracycline, because 96.66% of the isolates were susceptible against it. This is the first report of the isolation, seasonal distribution and antimicrobial susceptibility of Arcobacter species in cattle slaughterhouse samples in Turkey. These results indicate that foods of animal origin and cattle slaughterhouses are significant source of the antimicrobial resistant arcobacters.

  5. The impact of fecal sample processing on prevalence estimates for antibiotic-resistant Escherichia coli.

    Science.gov (United States)

    Omulo, Sylvia; Lofgren, Eric T; Mugoh, Maina; Alando, Moshe; Obiya, Joshua; Kipyegon, Korir; Kikwai, Gilbert; Gumbi, Wilson; Kariuki, Samuel; Call, Douglas R

    2017-05-01

    Investigators often rely on studies of Escherichia coli to characterize the burden of antibiotic resistance in a clinical or community setting. To determine if prevalence estimates for antibiotic resistance are sensitive to sample handling and interpretive criteria, we collected presumptive E. coli isolates (24 or 95 per stool sample) from a community in an urban informal settlement in Kenya. Isolates were tested for susceptibility to nine antibiotics using agar breakpoint assays and results were analyzed using generalized linear mixed models. We observed a 0.1). Prevalence estimates did not differ for five distinct E. coli colony morphologies on MacConkey agar plates (P>0.2). Successive re-plating of samples for up to five consecutive days had little to no impact on prevalence estimates. Finally, culturing E. coli under different conditions (with 5% CO 2 or micro-aerobic) did not affect estimates of prevalence. For the conditions tested in these experiments, minor modifications in sample processing protocols are unlikely to bias estimates of the prevalence of antibiotic-resistance for fecal E. coli. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Related antimicrobial resistance genes detected in different bacterial species co-isolated from swine fecal samples.

    Science.gov (United States)

    Frye, Jonathan G; Lindsey, Rebecca L; Meinersmann, Richard J; Berrang, Mark E; Jackson, Charlene R; Englen, Mark D; Turpin, Jennifer B; Fedorka-Cray, Paula J

    2011-06-01

    A potential factor leading to the spread of antimicrobial resistance (AR) in bacteria is the horizontal transfer of resistance genes between bacteria in animals or their environment. To investigate this, swine fecal samples were collected on-farm and cultured for Escherichia coli, Salmonella enterica, Campylobacter spp., and Enterococcus spp. which are all commonly found in swine. Forty-nine of the samples from which all four bacteria were recovered were selected yielding a total of 196 isolates for analysis. Isolates were tested for antimicrobial susceptibility followed by hybridization to a DNA microarray designed to detect 775 AR-related genes. E. coli and Salmonella isolated from the same fecal sample had the most AR genes in common among the four bacteria. Genes detected encoded resistance to aminoglycosides (aac(3), aadA1, aadB, and strAB), β-lactams (ampC, ampR, and bla(TEM)), chloramphenicols (cat and floR), sulfanillic acid (sul1/sulI), tetracyclines (tet(A), tet(D), tet(C), tet(G), and tet(R)), and trimethoprim (dfrA1 and dfh). Campylobacter coli and Enterococcus isolated from the same sample frequently had tet(O) and aphA-3 genes detected in common. Almost half (47%) of E. coli and Salmonella isolated from the same fecal sample shared resistance genes at a significant level (χ², p genes between these bacteria or there may be a common source of AR genes in the swine environment for E. coli and Salmonella.

  7. Development of resistance type moisture measuring device for grains

    Directory of Open Access Journals (Sweden)

    Sadiq Sius LAWAL

    2017-06-01

    Full Text Available The paper presents a study aimed at developing an indigenous moisture measuring device from locally available materials for grains based on the principle of resistance change of the material with corresponding change in moisture during drying. The developed device has four copper electrode cups positions in which both gauging and dummy copper electrode cups were placed to form Wheatstone bridge circuits of full, half and quarter for four, two and one gauging copper electrode cups respectively, while the remaining positions were used as dummies. These circuits were used to measure the resistances and hence obtaining the moisture content of the grains used. Grains of paddy rice, guinea corn and millet were used to test the device and it was observed that for full bridge circuits of each of the grains, paddy rice have a negative coefficient of correlation of -0.95, -0.99 for guinea corn and -0.99 for millet. The results obtained from predictive empirical equations when compared with the direct oven method, showed that the device can be used to predict or measure moisture content once calibrated.

  8. Resistivity measurements on the neutron irradiated detector grade silicon materials

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zheng

    1993-11-01

    Resistivity measurements under the condition of no or low electrical field (electrical neutral bulk or ENB condition) have been made on various device configurations on detector grade silicon materials after neutron irradiation. Results of the measurements have shown that the ENB resistivity increases with neutron fluence ({Phi}{sub n}) at low {phi}{sub n} (<10{sup 13} n/cm{sup 2}) and saturates at a value between 300 and 400 k{Omega}-cm at {phi}{sub n} {approximately}10{sup 13} n/cm{sup 2}. Meanwhile, the effective doping concentration N{sub eff} in the space charge region (SCR) obtained from the C-V measurements of fully depleted p{sup +}/n silicon junction detectors has been found to increase nearly linearly with {phi}{sub n} at high fluences ({phi}{sub n} > 10{sup 13} n/cm{sup 2}). The experimental results are explained by the deep levels crossing the Fermi level in the SCR and near perfect compensation in the ENB by all deep levels, resulting in N{sub eff} (SCR) {ne} n or p (free carrier concentrations in the ENB).

  9. Effect of Macroscopic Impurities on Resistive Measurements in Three Dimensions

    Science.gov (United States)

    Koon, Daniel W.

    1997-03-01

    The authors extend their study of the effect of macroscopic impurities on resistive measurements to include specimens of finite thickness. The effect of such impurities is calculated for a rectangular parallelepiped with two current and two voltage contacts on the corners of one of its faces. The weighting function(D. W. Koon and C. J. Knickerbocker, Rev. Sci. Instrum. 63, 207 (1992).) displays singularities near these contacts, but these are shown to vanish in the two-dimensional limit, in agreement with previous results.

  10. Laboratory electrical resistivity analysis of geologic samples from Fort Irwin, California: Chapter E in Geology and geophysics applied to groundwater hydrology at Fort Irwin, California

    Science.gov (United States)

    Bloss, Benjamin R.; Bedrosian, Paul A.

    2015-01-01

    Correlating laboratory resistivity measurements with geophysical resistivity models helps constrain these models to the geology and lithology of an area. Throughout the Fort Irwin National Training Center area, 111 samples from both cored boreholes and surface outcrops were collected and processed for laboratory measurements. These samples represent various lithologic types that include plutonic and metamorphic (basement) rocks, lava flows, consolidated sedimentary rocks, and unconsolidated sedimentary deposits that formed in a series of intermountain basins. Basement rocks, lava flows, and some lithified tuffs are generally resistive (≥100 ohm-meters [Ω·m]) when saturated. Saturated unconsolidated samples are moderately conductive to conductive, with resistivities generally less than 100 Ω·m, and many of these samples are less than 50 Ω·m. The unconsolidated samples can further be separated into two broad groups: (1) younger sediments that are moderately conductive, owing to their limited clay content, and (2) older, more conductive sediments with a higher clay content that reflects substantial amounts of originally glassy volcanic ash subsequently altered to clay. The older sediments are believed to be Tertiary. Time-domain electromagnetic (TEM) data were acquired near most of the boreholes, and, on the whole, close agreements between laboratory measurements and resistivity models were found. 

  11. An antithetic variate to facilitate upper-stem height measurements for critical height sampling with importance sampling

    Science.gov (United States)

    Thomas B. Lynch; Jeffrey H. Gove

    2013-01-01

    Critical height sampling (CHS) estimates cubic volume per unit area by multiplying the sum of critical heights measured on trees tallied in a horizontal point sample (HPS) by the HPS basal area factor. One of the barriers to practical application of CHS is the fact that trees near the field location of the point-sampling sample point have critical heights that occur...

  12. Freeze core sampling to validate time-lapse resistivity monitoring of the hyporheic zone.

    Science.gov (United States)

    Toran, Laura; Hughes, Brian; Nyquist, Jonathan; Ryan, Robert

    2013-01-01

    A freeze core sampler was used to characterize hyporheic zone storage during a stream tracer test. The pore water from the frozen core showed tracer lingered in the hyporheic zone after the tracer had returned to background concentration in collocated well samples. These results confirmed evidence of lingering subsurface tracer seen in time-lapse electrical resistivity tomographs. The pore water exhibited brine exclusion (ion concentrations in ice lower than source water) in a sediment matrix, despite the fast freezing time. Although freeze core sampling provided qualitative evidence of lingering tracer, it proved difficult to quantify tracer concentration because the amount of brine exclusion during freezing could not be accurately determined. Nonetheless, the additional evidence for lingering tracer supports using time-lapse resistivity to detect regions of low fluid mobility within the hyporheic zone that can act as chemically reactive zones of importance in stream health. © 2012, The Author(s). GroundWater © 2012, National Ground Water Association.

  13. Prevalence of Methicillin?Resistant Staphylococcus aureus from Equine Nasopharyngeal and Guttural Pouch Wash Samples

    OpenAIRE

    Boyle, A.G.; Rankin, S.C.; Duffee, L.A.; Morris, D.

    2017-01-01

    Background Methicillin?resistant Staphylococcus aureus (MRSA) is recognized as a cause of nosocomial infections in both human and veterinary medicine. Studies that examine the nasopharynx and guttural pouches of the horse as carriage sites for MRSA have not been reported. Hypothesis/Objective MRSA colonizes the nasopharynx and guttural pouch of horses. To determine the prevalence of MRSA in equine nasopharyngeal wash (NPW) and guttural pouch lavage (GPL) samples in a field population of horse...

  14. Earthquake sequence simulations with measured properties for JFAST core samples

    Science.gov (United States)

    Noda, Hiroyuki; Sawai, Michiyo; Shibazaki, Bunichiro

    2017-08-01

    Since the 2011 Tohoku-Oki earthquake, multi-disciplinary observational studies have promoted our understanding of both the coseismic and long-term behaviour of the Japan Trench subduction zone. We also have suggestions for mechanical properties of the fault from the experimental side. In the present study, numerical models of earthquake sequences are presented, accounting for the experimental outcomes and being consistent with observations of both long-term and coseismic fault behaviour and thermal measurements. Among the constraints, a previous study of friction experiments for samples collected in the Japan Trench Fast Drilling Project (JFAST) showed complex rate dependences: a and a-b values change with the slip rate. In order to express such complexity, we generalize a rate- and state-dependent friction law to a quadratic form in terms of the logarithmic slip rate. The constraints from experiments reduced the degrees of freedom of the model significantly, and we managed to find a plausible model by changing only a few parameters. Although potential scale effects between lab experiments and natural faults are important problems, experimental data may be useful as a guide in exploring the huge model parameter space. This article is part of the themed issue 'Faulting, friction and weakening: from slow to fast motion'.

  15. Probe technologies for clean sampling and measurement of subglacial lakes.

    Science.gov (United States)

    Mowlem, Matt; Saw, Kevin; Brown, Robin; Waugh, Edward; Cardwell, Christopher L; Wyatt, James; Magiopoulos, Iordanis; Keen, Peter; Campbell, Jon; Rundle, Nicholas; Gkritzalis-Papadopoulos, Athanasios

    2016-01-28

    It is 4 years since the subglacial lake community published its plans for accessing, sampling, measuring and studying the pristine, and hitherto enigmatic and very different, Antarctic subglacial lakes, Vostok, Whillans and Ellsworth. This paper summarizes the contrasting probe technologies designed for each of these subglacial environments and briefly updates how these designs changed or were used differently when compared to previously published plans. A detailed update on the final engineering design and technical aspects of the probe for Subglacial Lake Ellsworth is presented. This probe is designed for clean access, is negatively buoyant (350 kg), 5.2 m long, 200 mm in diameter, approximately cylindrical and consists of five major units: (i) an upper power and communications unit attached to an optical and electrical conducting tether, (ii)-(iv) three water and particle samplers, and (v) a sensors, imaging and instrumentation pack tipped with a miniature sediment corer. To date, only in Subglacial Lake Whillans have instruments been successfully deployed. Probe technologies for Subglacial Lake Vostok (2014/15) and Lake Ellsworth (2012/13) were not deployed for technical reasons, in the case of Lake Ellsworth because hot-water drilling was unable to access the lake during the field season window. Lessons learned and opportunities for probe technologies in future subglacial access missions are discussed. © 2015 The Author(s).

  16. Microneedle-based minimally-invasive measurement of puncture resistance and fracture toughness of sclera.

    Science.gov (United States)

    Park, Seung Hyun; Lee, Kang Ju; Lee, JiYong; Yoon, Jae Hyoung; Jo, Dong Hyun; Kim, Jeong Hun; Kang, Keonwook; Ryu, WonHyoung

    2016-10-15

    The sclera provides the structural support of the eye and protects the intraocular contents. Since it covers a large portion of the eye surface and has relatively high permeability for most drugs, the sclera has been used as a major pathway for drug administration. Recently, microneedle (MN) technology has shown the possibility of highly local and minimally-invasive drug delivery to the eye by MN insertion through the sclera or the suprachoroidal space. Although ocular MN needs to be inserted through the sclera, there has been no systematic study to understand the mechanical properties of the sclera, which are important to design ocular MNs. In this study, we investigated a MN-based method to measure the puncture resistance and fracture toughness of the sclera. To reflect the conditions of MN insertion into the sclera, force-displacement curves obtained from MN-insertion tests were used to estimate the puncture resistance and fracture toughness of sclera tissue. To understand the effect of the insertion conditions, dependency of the mechanical properties on insertion speeds, pre-strain of the sclera, and MN sizes were analyzed and discussed. Measurement of mechanical property of soft biological tissue is challenging due to variations between tissue samples or lack of well-defined measurement techniques. Although non-invasive measurement techniques such as nano/micro indentation were employed to locally measure the elastic modulus of soft biological materials, mechanical properties such as puncture resistance or fracture toughness, which requires "invasive" measurement and is important for the application of "microneedles or hypodermic needles", has not been well studied. In this work, we report minimally-invasive measurement of puncture resistance and fracture toughness of sclera using a double MN insertion method. Parametric studies showed that use of MN proved to be advantageous because of minimally-invasive insertion into tissue as well as higher sensitivity to

  17. Geometric factor and influence of sensors in the establishment of a resistivity-moisture relation in soil samples

    Science.gov (United States)

    López-Sánchez, M.; Mansilla-Plaza, L.; Sánchez-de-laOrden, M.

    2017-10-01

    Prior to field scale research, soil samples are analysed on a laboratory scale for electrical resistivity calibrations. Currently, there are a variety of field instruments to estimate the water content in soils using different physical phenomena. These instruments can be used to develop moisture-resistivity relationships on the same soil samples. This assures that measurements are performed on the same material and under the same conditions (e.g., humidity and temperature). A geometric factor is applied to the location of electrodes, in order to calculate the apparent electrical resistivity of the laboratory test cells. This geometric factor can be determined in three different ways: by means of the use of an analytical approximation, laboratory trials (experimental approximation), or by the analysis of a numerical model. The first case, the analytical approximation, is not appropriate for complex cells or arrays. And both, the experimental and numerical approximation can lead to inaccurate results. Therefore, we propose a novel approach to obtain a compromise solution between both techniques, providing a more precise determination of the geometrical factor.

  18. Elevation of cardiac troponins measured after recreational resistance training.

    Science.gov (United States)

    Savukoski, Tanja; Mehtälä, Laura; Lindahl, Bertil; Venge, Per; Pettersson, Kim

    2015-08-01

    Whereas elevated cardiac troponin (cTn) concentrations i.e. above the 99th percentile of healthy reference population (recommended cutoff for the diagnosis of myocardial infarction) are well-documented in healthy individuals after prolonged and/or intensive exercises such as marathons, data on less-strenuous sports are scarce. Therefore, our aim was to investigate cTnI and cTnT release in response to recreational resistance training, here a single-bout of 1-h kettlebell workout. Serum samples were collected from 11 apparently healthy volunteers the previous day (pre-exercise), three hours after the kettlebell class (post-exercise), the next day and three days later. The aliquoted samples were analyzed with Abbott Laboratories' Architect high-sensitivity (hs)-cTnI assay (limit of detection, LoD = 2 ng/L), our 3+1-type cTnI assay free from cTn-specific autoantibody interference (LoD = 3 ng/L) and Roche Diagnostics' hs-cTnT assay (LoD = 5 ng/L). The post-exercise cTn concentrations were significantly higher than the pre-exercise values (median 5.5-9.6 ng/L vs. 26 ng/L, n = 2) and/or hs-cTnT (>14 ng/L, n = 4). The cTn concentrations returned to baseline during the three days of follow-up. Our study demonstrates abnormally elevated cTns with well-validated sensitive cTn assays after resistance training. This confirms that different kinds of recreational physical activity are yet another confounder that may affect the determination and use of 99th percentile reference values. Therefore, exercise-associated changes should be carefully addressed as part of the evaluation what is "normal cTn". Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  19. Passive measurement of flux nucleation in the current-induced resistive state of type I superconductors

    International Nuclear Information System (INIS)

    Selig, K.P.; Chimenti, D.E.; Huebener, R.P.

    1978-01-01

    Flux-tube nucleation rates have been measured in the current-induced resistive state of type I superconducting In films between 1.5 and 2.0 K by a completely passive technique. Indication of periodic nucleation is observed only in narrow regions of sample voltage drop, whose position is a sensitive function of temperature. Frequency bandwidth measurements of the nucleation rate yield a spectral purity of one part in 10 4 within the narrow regions where an experimental signal can be detected. (orig.) [de

  20. Short communication: Prevalence, antimicrobial resistance, and resistant traits of coagulase-negative staphylococci isolated from cheese samples in Turkey.

    Science.gov (United States)

    Kürekci, Cemil

    2016-04-01

    A total of 17 coagulase-negative staphylococci (CNS) isolates obtained from 72 cheese samples were included in this study. Coagulase-negative staphylococci isolates obtained in this study comprised 6 (35.3%) Staphylococcus saprophyticus, 3 (17.6%) Staphylococcus epidermidis, 2 (11.8%) Staphylococcus hominis, 2 (11.8%) Staphylococcus haemolyticus, 1 (5.9%) Staphylococcus xylosus, 1 (5.9%) Staphylococcus vitulinus, 1 (5.9%) Staphylococcus lentus, and 1 (5.9%) Staphylococcus warneri. The disc diffusion assay revealed that the highest occurrence of resistance was found for penicillin (76.5%), erythromycin (35.3%), tetracycline (29.4%), and trimethoprim-sulfamethoxazole (17.6%) among CNS isolates. However, all CNS isolates were found to be susceptible to vancomycin, streptomycin, linezolid, and gentamycin. Of the isolates, 64.7% carried at least one of the following antimicrobial resistance genes: mecA, tet(M), erm(B), blaZ, ant(4')-la, aph(3')-IIIa, and lnu(A). The results suggest that improved hygienic conditions, such as safer handling of raw milk, proper cleaning, and sanitation during the manufacturing in the dairies, are urgently needed in Turkey. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  1. Resistência do solo à penetração em resposta ao número de amostras e tipo de amostragem Variation in soil penetration resistance in response to sample number and sampling type

    Directory of Open Access Journals (Sweden)

    João Tavares Filho

    2008-04-01

    com o sistema de manejo e a profundidade de amostragem. A melhor representatividade da média dos resultados de resistência do solo à penetração ocorreu para n > 15 (PD e CP ou n > 20 (0-0,10 m e 15 (0,20-0,60 m no caso do PC. A população amostral n > 10 na profundidade 0-0,60 m, nos dois tipos de amostragem e manejos de solo, permitiu alta exatidão dos dados, tornando os parâmetros estatísticos mais confiáveis, com homogeneidade nos resultados e linearidade apresentada nas curvas das populações amostrais a partir do erro amostral de 10 %Monitoring the state of soil compaction periodically by assessing soil penetration resistance is a practical way of evaluating the effects of different management systems on the soil structure and crop root development. This study aimed to evaluate the variation of soil penetration resistance in response to the number of replications (sample population of different field sampling forms of an Oxisol under three management types: non-tillage (PD, perennial crop (CP and conventional tillage (PC. The experiment was carried out in the Northern Paraná State, Brazil. Samples were collected in three sub-areas of 1ha to determine soil penetration resistance at different depths (0-0.10; 0.10-0.20; 0.20-0.40; 0.40-0.60 m. Sampling was carried out as follows: systematic sampling (grid points, spaced 25 m apart and completely randomized sampling, with 1, 3, 5, 10, 15, 20, 30, 40, and 50 replications. For all points and depths it was determined the average value of penetration resistance (MPa, the confidence interval and estimation accuracy (D of the penetrometer measurements through classical statistical theory based on the number of samples (n and standard deviation of the sample (S, at a significance level of 0.05. For the given experimental conditions (sub-areas of 1ha Oxisol under three different managements, results indicated that the number of representative samples to determine soil penetration resistance did not vary

  2. Evaluation of the Mechanical Properties of Gray Cast Iron Using Electrical Resistivity Measurement

    Directory of Open Access Journals (Sweden)

    Bieroński M.

    2016-12-01

    Full Text Available In this paper an attempt to determine the relationship between the electrical resistivity and the tensile strength and hardness of cast iron of carbon equivalent in the range from 3.93% to 4.48%. Tests were performed on the gray cast iron for 12 different melts with different chemical composition. From one melt poured 6 samples. Based on the study of mechanical and electro-resistive determined variation characteristics of tensile strength, hardness and resistivity as a function of the carbon equivalent. Then, regression equations were developed as power functions describing the relationship between the resistivity of castings and their tensile strength and hardness. It was found a high level of regression equations to measuring points, particularly with regard to the relationship Rm=f(ρ. The obtained preliminary results indicate the possibility of application of the method of the resistance to rapid diagnostic casts on the production line, when we are dealing with repeatable production, in this case non variable geometry of the product for which it has been determinated before a regression equation.

  3. Prevalence of Methicillin-Resistant Staphylococcus aureus from Equine Nasopharyngeal and Guttural Pouch Wash Samples.

    Science.gov (United States)

    Boyle, A G; Rankin, S C; Duffee, L A; Morris, D

    2017-09-01

    Methicillin-resistant Staphylococcus aureus (MRSA) is recognized as a cause of nosocomial infections in both human and veterinary medicine. Studies that examine the nasopharynx and guttural pouches of the horse as carriage sites for MRSA have not been reported. MRSA colonizes the nasopharynx and guttural pouch of horses. To determine the prevalence of MRSA in equine nasopharyngeal wash (NPW) and guttural pouch lavage (GPL) samples in a field population of horses. One hundred seventy-eight samples (123 NPW and 55 GPL) from 108 horses. Prospective study. Samples were collected from a convenience population of clinically ill horses with suspected Streptococcus equi subsp. equi (S. equi) infection, horses convalescing from a known S. equi infection, and asymptomatic horses undergoing S. equi screening. Samples were submitted for S. aureus aerobic bacterial culture with mannitol salt broth and two selective agars (cefoxitin CHROMagar as the PBP2a inducer and mannitol salt agar with oxacillin). Biochemical identification of Staphylococcus species and pulsed-field gel electrophoresis (PFGE), to determine clonal relationships between isolates, were performed. Methicillin-resistant Staphylococcus (MRS) was isolated from the nasopharynx of 7/108 (4%) horses. Three horses had MRSA (2.7%), and 4 had MR-Staphylococcus pseudintermedius (MRSP). MRSA was isolated from horses on the same farm. PFGE revealed the 3 MRSA as USA 500 strains. Sampling the nasopharynx and guttural pouch of community-based horses revealed a similarly low prevalence rate of MRSA as other studies sampling the nares of community-based horses. More study is required to determine the need for sampling multiple anatomic sites when screening horses for MRSA. Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

  4. Antimicrobial sensitivity and resistance of Salmonella Enteritidis isolated from natural samples

    Directory of Open Access Journals (Sweden)

    Madhavarao Thirtham

    2013-08-01

    Full Text Available Aim: To test the sensitivity of S. Enteritidis for selected antibiotics. Materials and Methods: S. Enteritidis isolates obtained from different samples of chicken, mutton, turkey meat, faecal and cloacal samples of poultry and turkey, eggs, water and feed were subjected for sensitivity and resistance to selected antibiotics like- Chloramphenicol (30μg, Gentamicin (10 μg, Nalidixic Acid (30 μg, Tetracycline (30 μg, Ciprofloxacin (5 μg, Amikacin (30 μg, Amoxicillin (25 μg, Ampicillin (10 μg, Streptomycin (10 μg and Sulfonamide (30 μg. Antimicrobial susceptibility of the isolates was established by the disk diffusion assay with MH (Muller-Hinton agar in accordance with French National Antibiogram Committee Guidelines. Results: The sensitivity of S. Enteritidis was 100% for ciprofloxacin followed by chloramphenicol and amikacin (96%, gentamycin (90%, amoxicillin (82%, streptomycin (80%, tetracycline (76%, nalidixic acid (68%, ampicillin (58% and sulfonamide (10%. The resistance was highest for sulfonamide (76% followed by ampicillin (32%, nalidixic acid (30% and 6-20% for gentamycin, amoxicillin and tetracycline. Conclusion: S. Enteritidis isolates were more sensitive to ciprofloxacin, chloramphenicol, amikacin, gentamycin, streptomycin,amoxicillin and tetracyclines and less sensitive to sulfonamides. Higher resistance was observed with sulfonamide followed by ampicillin and nalidixic acid. [Vet World 2013; 6(4.000: 185-188

  5. Measurement of 90Sr in fresh water samples

    International Nuclear Information System (INIS)

    Belanova, A.; Meresova, J.; Svetlik, I.; Tomaskova, L.

    2008-01-01

    This preliminary study show new experimental approach to the determination of the radionuclide 90 Sr in water samples. The new method of dynamic windows utilizing liquid scintillation counting was applied on model and surface water samples. Our results show the demand of separation technique with significantly higher yields. (authors)

  6. Multidrug-resistant bacteria isolated from intensive-care-unit patient samples

    Directory of Open Access Journals (Sweden)

    Aziz Japoni

    Full Text Available We examined epidemiological aspects and bacterial resistance patterns of bacteria isolated from intensive care unit (ICU patient samples. During a 10 month period (from June 2006 to March 2007, 812 samples of blood, urine and cerebral spinal fluid (CSF from 553 hospitalized patients, in ICU wards, including pediatric surgical, neonatal, adult surgical I, adult surgical II, general pediatrics, neurosurgical I, neurosurgical II, and internal medical, were collected. Minimum inhibitory concentration (MIC of antibiotics for bacteria isolates was determined by the E-test method. The internal medicine ICU with 28.7% admissions gave the largest contribution. Coagulase negative staphylococci at frequencies of 66.7 % and 36.5 % and E. coli at 20.9% were the bacteria most frequently isolated from the blood, CSF and urine samples, respectively. Samples taken from patients 20-40 years old were the most frequent (32.2%, while the group of patients over sixty years contributed least (18.5%. Both Gram-positive and - negative isolates expressed resistance to most of the penicillins and cephalosporins tested. Combined therapy with vancomycin and meropenem or imipenem gave the most effective treatment against Gram-positive and Gram-negative isolates based on empirical therapy. High frequencies of multiresistant bacteria in ICUs warn us to administer a few effective antibiotics in our hospitals more wisely in order to reduce selective pressure on sensitive strains. This could help save the life of ICU patients and prevent of spread of resistant isolates in these critical wards. Due to continuous changes in antibacterial susceptibility patterns, periodical antibacterial sensitivity assessment in ICUs should be mandatory.

  7. AISI 304 Welding Fracture Resistance by a Charpy Impact Test with a High Speed Sampling Rate

    Directory of Open Access Journals (Sweden)

    Bambang Riyanta

    2017-12-01

    Full Text Available The purpose of this study was to evaluate fracture resistance in AISI 304. The J-R curve was constructed from data, which resulted from an impact test by Charpy Impact machine equipped with high-speed sampling rate data acquisition equipment. The critical values of fracture resistance in fusion zones (FZ, high temperature heat affected zones (HTHAZ, low temperature heat affected zones (LTHAZ and unaffected base metals (UBM were obtained by calculation methods using some formulas and by graphical methods. Laboratory experiments demonstrated the relationships among the values of energy absorption along the impact test with the obstruction of dislocation movement due to the presence of chromium interstitial solute in all zones and chromium rich carbide precipitates in fusion zones and heat affected zones.

  8. Sampling, prevalence and characterization of methicillin-resistant Staphylococcus aureus on two Belgian pig farms

    Directory of Open Access Journals (Sweden)

    Isabelle Dewaele

    2011-02-01

    Full Text Available This study investigated the spread of MRSA (methicillin-resistant Staphylococcus aureus on two Belgian pig farms. Pigs of different ages (from farrowing to slaughter age and sows as well as the barn environment were screened extensively on two occasions three months apart. A subset of MRSA isolates was tested for antimicrobial susceptibility to 16 antibiotics and was further characterized by pulsed-field gel electrophoresis. Ninety-five percent and 77% of the tested pigs on farm A and farm B, respectively, were colonized with MRSA. MRSA positive animals were detected in all age categories sampled on each sampling day. Piglets were already colonized in the farrowing unit with the same or other MRSA strains than their mother. The prevalence of MRSA colonized pigs increased significantly after weaning and decreased during the fattening period. Pigs carried MRSA mainly in the nares, followed by the perineum and skin and to a lesser degree the rectum. A pig could be contaminated or colonized with different MRSA strains at the same time. The barn environment was also found to be contaminated with different MRSA strains, including the air inlet and outlet. All isolates tested on both farms were resistant to both tetracycline and trimethoprim, while they were susceptible to rifampicin, mupirocin and linezolid. There was a significant difference in resistance prevalence between the two farms for the antibiotics gentamicin, kanamycin, tobramycin, tylosin, lincomycin and quinupristin/dalfopristin. Furthermore, several antibiotic resistance profiles were observed within one farm. This study clearly indicates that several MRSA strains circulate on one farm, from the nursery unit to the fattening unit. This is important to consider when attempts are made to remediate these farms.

  9. Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland.

    Science.gov (United States)

    Gindonis, Veera; Taponen, Suvi; Myllyniemi, Anna-Liisa; Pyörälä, Satu; Nykäsenoja, Suvi; Salmenlinna, Saara; Lindholm, Laura; Rantala, Merja

    2013-08-28

    Methicillin-resistant staphylococci (MRS) are increasingly being isolated in bovine mastitis. The aim of our study was to evaluate the occurrence of MRS in Finnish mastitis milk samples and characterize the MRS isolates using molecular methods. Methicillin-resistant S. aureus (MRSA) was a rare finding in bovine mastitis in Finland. Only two out of 135 (1.5%) S. aureus isolates were positive for mec genes. One of these carried mecA and was of spa type t172, SCCmec type IV and ST375, and the other harboured mecC, being spa type t3256, and ST130. MRSA ST375 is common among human MRSA isolates in Finland, but this is the first report in the country of bovine mecC MRSA. In coagulase-negative staphylococci (CoNS) originating from bovine mastitis, methicillin resistance was more common. In the two CoNS collections studied, 5.2% (17/324) and 1.8% (2/110) of the isolates were mecA positive. Eighteen of these were methicillin-resistant S. epidermidis (MRSE), which were divided into 6 separate PFGE clusters. One pulsotype was detected in different parts of the country, indicating clonal spread. Most MRSE (13/18) were of SCCmec type IV, one was of type V and four were non-typeable. Comparison with a human staphylococcal database indicated that bovine MRSE strains were not closely related to human MRSE isolates. The occurrence of MRS, especially MRSA, in bovine mastitis in Finland was low. Most methicillin-resistant bovine CoNS are MRSE, and we found evidence of a bovine MRSE strain that may spread clonally. This is the first report of a Finnish bovine isolate of MRSAmecC ST130. The study provides a baseline for further MRS monitoring.

  10. Thermal strain measurement of EAST W/Cu divertor structure using electric resistance strain gauges

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xingli [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Science Island Branch of Graduate School, University of Science & Technology of China, Hefei, 230031 (China); Wang, Wanjing, E-mail: wjwang@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Wang, Jichao [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Wei, Ran; Sun, Zhaoxuan [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Science Island Branch of Graduate School, University of Science & Technology of China, Hefei, 230031 (China); Li, Qiang; Xie, Chunyi [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Chen, Hong-En; Wang, Kaiqiang; Wu, Lei; Chen, Zhenmao [State Key Lab for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University (China); Luo, Guang-Nan [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Science Island Branch of Graduate School, University of Science & Technology of China, Hefei, 230031 (China); Hefei Center for Physical Science and Technology, Hefei, 230022 (China); Hefei Science Center of Chinese Academy of Sciences, Hefei, 230027 (China)

    2016-12-15

    Highlights: • To understand the service behavior of W/Cu divertor, an electrical resistance strain gauge system had been introduced in a thermal strain measurement experiment. • The measurement system successfully finished the experiment and obtained valued thermal strain data. • Two thermomechanical analyses had also been carried out and compared with the measurement results. • Experiment results corresponded well to simulations and threw a light upon the failure of W/Cu divertor in the previous baking tests. - Abstract: W/Cu divertor has complex structure and faces extreme work environment in EAST Tokamak device. To measure its thermal strain shall be a valued way to understand its service behavior and then optimize its design and manufacturing process. This work presents a preliminary study on measuring thermal strain of EAST W/Cu divertor structure using electric resistance strain gauges. Eight gauges had been used in the experiment and the heating temperature had been set to 230 °C with respect to the work temperature. To realize the measuring experiment, an appropriate fixing method of gauges in divertor narrow spaces had been taken and tested, which could not only withstand high temperature but also had no damage to the divertor sample. The measurement results were that three gauges showed positive strain while other three showed negative strain after having been compensated, which corresponded to tensile stress and compressed stress respectively. Two thermomechanical simulations had also been carried out and used for comparing with the experiment.

  11. The epidemiology and molecular characterization of methicillin-resistant staphylococci sampled from a healthy Jordanian population.

    Science.gov (United States)

    Al-Bakri, A G; Al-Hadithi, H; Kasabri, V; Othman, G; Kriegeskorte, A; Becker, K

    2013-11-01

    The prevalence of natural carriage and molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolates in a Jordanian community were investigated. The MRSA nasal carriage rate in 227 healthy volunteers was 7·5% and the majority (81%) of MRSA harboured the resistance element SCCmec type IVe and were of a novel spa type t9519 (76%); other significant spa gene types were t223 (14·7%) and t044 (5·9%). All MRSA isolates were susceptible to other classes of antibiotics, and tested positive for at least three virulence factor encoding genes, but only two harboured the pvl gene. MR-CoNS carriage was 54·2% and these isolates were characterized by single, double and untypable SCCmec elements, with Staphylococcus epidermidis SCCmec type IVa predominating. Of eight subjects with nasal co-colonization of MR-CoNS + MRSA, three shared SCCmec type IV in both groups of organisms. This is the first report of methicillin-resistant staphylococci carriage in a Jordanian community and its findings are important for epidemiological study and infection control measures of these organisms.

  12. In situ measurement on TSV-Cu deformation with hotplate system based on sheet resistance

    Science.gov (United States)

    Sun, Yunna; Wang, Bo; Wang, Huiying; Wu, Kaifeng; Yang, Shengyong; Wang, Yan; Ding, Guifu

    2017-12-01

    The in situ measurement of TSVs deformation at different temperature is meaningful for learning more about the thermal deformation schemes of 3D TSVs in the microelectronic devices. An efficient and smart hotplate based on sheet resistance is designed for offering more heat, producing a uniform temperature distribution, relieving thermal stress and heat concentration issues, and reducing room space, which was optimized by the finite element method (FEM). The fabricated hotplate is efficient and smart (2.5 cm  ×  2.0 cm  ×  0.5 cm) enough to be located in the limited space during measuring. The thermal infrared imager was employed as the temperature sensor for monitoring the temperature distribution of TSVs sample. The 3D profilometry was adopted as the observer for TSVs profiles survey. The in situ 2D top surface profiles and 3D displacement profiles of TSVs sample at the different temperature were measured by 3D profilometer. The in situ average relative deformation and effective plastic deformation of the TSV sample were measured. With optical measurement method, 3D profilometry, the TSV sample can be tested repeatedly.

  13. Prevalence, quantitative load, and antimicrobial resistance of Campylobacter spp. from broiler ceca and broiler skin samples in Thailand.

    Science.gov (United States)

    Chokboonmongkol, C; Patchanee, P; Gölz, G; Zessin, K-H; Alter, T

    2013-02-01

    This study was conducted to determine the prevalence of Campylobacter spp. in broiler flocks by testing cecal contents at slaughter and to detect and quantify Campylobacter on broiler carcass skin samples of the corresponding slaughter batches, to determine antimicrobial resistance patterns of the Campylobacter isolates, and to genotype selected Campylobacter jejuni isolates using multilocus sequence typing analysis. Ninety-eight broiler flocks were included in the study. Intact ceca were randomly taken at the time of evisceration throughout a slaughter batch to detect Campylobacter spp. at the broiler flock level and one whole carcass per slaughter batch was taken for the detection of Campylobacter spp. on broiler skin. The prevalences of Campylobacter spp. in broiler ceca and broiler skin samples were 11.2% (11/98) and 51% (50/98), respectively. Even though most Campylobacter-positive broiler skin samples were contaminated with only up to 230 most probable number per gram, a substantial share (13.3%) showed very high Campylobacter numbers on the broiler skin samples (most probable number = ∞; lower confidence limit T(0) 580/g). From 32 C. jejuni and Campylobacter coli isolates tested, the highest antimicrobial resistance rates were found for ciprofloxacin (81.2%), followed by tetracycline (40.6%), ampicillin (31.2%), and erythromycin (9.4%). All tested strains were sensitive to gentamicin. By multilocus sequence typing analysis, a total of 9 different sequence types were identified among 16 C. jejuni isolates. Campylobacter jejuni isolated from cecal content and carcass skin of the same farm or slaughter batch showed corresponding allelic profiles. Our data suggest that intense cross-contamination during the slaughter process led to a strong increase of Campylobacter prevalence on broiler skin compared with the prevalence in broiler ceca. To reduce Campylobacter prevalences on broiler skin, on-farm biosecurity measures need to be accompanied by control measures

  14. Measurement assurance program for FTIR analyses of deuterium oxide samples

    International Nuclear Information System (INIS)

    Johnson, S.R.; Clark, J.P.

    1997-01-01

    Analytical chemistry measurements require an installed criterion based assessment program to identify and control sources of error. This program should also gauge the uncertainty about the data. A self- assessment was performed of long established quality control practices against the characteristics of a comprehensive measurement assurance program. Opportunities for improvement were identified. This paper discusses the efforts to transform quality control practices into a complete measurement assurance program. The resulting program heightened the laboratory's confidence in the data it generated, by providing real-time statistical information to control and determine measurement quality

  15. Multidrug-resistant tuberculosis: Rapid molecular detection with MTBDRplus® assay in clinical samples

    Directory of Open Access Journals (Sweden)

    Rita Macedo

    2009-05-01

    Full Text Available Nowadays, the greatest concern of tuberculosis control programmes is the appearance of multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis. Rapid determination of drug resistance in clinical samples, with Mycobacterium tuberculosis complex (MTC, is the prerequisite for initiating effective chemotherapy, ensuring successful treatment of the patient and preventing further spread of drugresistant isolates.The aim of our study was to determine the sensitivity of the new MTBDRplus® assay in comparison to culture, identification and classic DST, directly from smear-positive clinical specimens.A total of 68 smear-positive sputum specimens were processed by both the classical mycobacteriological methods and the molecular assay, MTBDRplus®.MTBDRplus® assay allowed an accurate identification of MTC species by detection of the specific band in all samples, from which we also isolated and identified MTC strains by culture methods. In the samples from which we isolated susceptible strains (63.2%, wild type patterns were found using MTBDRplus® assay. The samples from which we isolated resistant strains (36.8% showed specific mutations associated with the correspondent resistant phenotype.Our study indicated that this assay allows rapid detection of resistance, always in agreement with classic methods. Resumo: Uma das principais problematicas no controlo da tuberculose e o aparecimento de casos de tuberculose multirresistente (TB-MR e tuberculose extensivamente resistente (TB-XDR. A deteccao precoce da resistencia a farmacos, directamente a partir de amostras respiratorias, e essencial para que se assegure o tratamento atempado, adequado e eficaz da tuberculose, bem como para prevenir a disseminacao destes casos de especial gravidade.O nosso objectivo foi avaliar a sensibilidade e comparar os resultados obtidos com um metodo de genetica molecular disponivel comercialmente – MTBDRplus® – e o isolamento

  16. Measurement of radon daughters in air samples by alpha spectroscopy

    International Nuclear Information System (INIS)

    Acena, M.L.; Crespo, M.T.

    1989-01-01

    The concentration of radon progeny in air has been determined by alpha spectrometry measurement of polonium 214 and polonium 218. A known volume of air was passed through a filter, then the alpha activity was directly measured on this filter (Author)

  17. Adaptive Sampling for High Throughput Data Using Similarity Measures

    Energy Technology Data Exchange (ETDEWEB)

    Bulaevskaya, V. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Sales, A. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-05-06

    The need for adaptive sampling arises in the context of high throughput data because the rates of data arrival are many orders of magnitude larger than the rates at which they can be analyzed. A very fast decision must therefore be made regarding the value of each incoming observation and its inclusion in the analysis. In this report we discuss one approach to adaptive sampling, based on the new data point’s similarity to the other data points being considered for inclusion. We present preliminary results for one real and one synthetic data set.

  18. Measurement, Construction, and Maintenance of Skid-Resistant Airport Pavement Surfaces

    Science.gov (United States)

    1997-03-18

    This advisory circular (AC) contains guidelines and procedures for the design : and construction of skid-resistant pavement, pavement evaluation with friction : measuring equipment, and maintenance of high skid-resistant pavements. 45p.

  19. Three sampling methods for visibility measures of landscape perception

    NARCIS (Netherlands)

    Weitkamp, S.G.; Bregt, A.K.; Lammeren, van R.J.A.; Berg, van den A.E.

    2007-01-01

    The character of a landscape can be seen as the outcome of people¿s perception of their physical environment, which is important for spatial planning and decision making. Three modes of landscape perception are proposed: view from a viewpoint, view from a road, and view of an area. Three sampling

  20. Measuring Student Achievement in Travel and Tourism. Sample Test Questions.

    Science.gov (United States)

    New York State Education Dept., Albany. Bureau of Business Education.

    The sample test items included in this document are intended as a resource for teachers of Marketing and Distributive Education programs with emphasis on hospitality and recreation marketing, and tourism and travel services marketing. The related curriculum material has been published in the Travel and Tourism syllabus, an advanced-level module in…

  1. Fiber sample presentation system for spectrophotometer cotton fiber color measurements

    Science.gov (United States)

    The Uster® High Volume Instrument (HVI) is used to class U.S. cotton for fiber color, yielding the industry accepted, cotton-specific color parameters Rd and +b. The HVI examines a 9 square inch fiber sample, and it is also used to test large AMS standard cotton “biscuits” or rectangles. Much inte...

  2. Electric field computation and measurements in the electroporation of inhomogeneous samples

    Science.gov (United States)

    Bernardis, Alessia; Bullo, Marco; Campana, Luca Giovanni; Di Barba, Paolo; Dughiero, Fabrizio; Forzan, Michele; Mognaschi, Maria Evelina; Sgarbossa, Paolo; Sieni, Elisabetta

    2017-12-01

    In clinical treatments of a class of tumors, e.g. skin tumors, the drug uptake of tumor tissue is helped by means of a pulsed electric field, which permeabilizes the cell membranes. This technique, which is called electroporation, exploits the conductivity of the tissues: however, the tumor tissue could be characterized by inhomogeneous areas, eventually causing a non-uniform distribution of current. In this paper, the authors propose a field model to predict the effect of tissue inhomogeneity, which can affect the current density distribution. In particular, finite-element simulations, considering non-linear conductivity against field relationship, are developed. Measurements on a set of samples subject to controlled inhomogeneity make it possible to assess the numerical model in view of identifying the equivalent resistance between pairs of electrodes.

  3. Reexamination of basal plane thermal conductivity of suspended graphene samples measured by electro-thermal micro-bridge methods

    Directory of Open Access Journals (Sweden)

    Insun Jo

    2015-05-01

    Full Text Available Thermal transport in suspended graphene samples has been measured in prior works and this work with the use of a suspended electro-thermal micro-bridge method. These measurement results are analyzed here to evaluate and eliminate the errors caused by the extrinsic thermal contact resistance. It is noted that the room-temperature thermal resistance measured in a recent work increases linearly with the suspended length of the single-layer graphene samples synthesized by chemical vapor deposition (CVD, and that such a feature does not reveal the failure of Fourier’s law despite the increase in the reported apparent thermal conductivity with length. The re-analyzed apparent thermal conductivity of a single-layer CVD graphene sample reaches about 1680 ± 180 W m−1 K−1 at room temperature, which is close to the highest value reported for highly oriented pyrolytic graphite. In comparison, the apparent thermal conductivity values measured for two suspended exfoliated bi-layer graphene samples are about 880 ± 60 and 730 ± 60 Wm−1K−1 at room temperature, and approach that of the natural graphite source above room temperature. However, the low-temperature thermal conductivities of these suspended graphene samples are still considerably lower than the graphite values, with the peak thermal conductivities shifted to much higher temperatures. Analysis of the thermal conductivity data reveals that the low temperature behavior is dominated by phonon scattering by polymer residue instead of by the lateral boundary.

  4. Transverse strength of railway tracks: part 2. Test system for ballast resistance in line measurement

    Directory of Open Access Journals (Sweden)

    Antonio De Iorio

    2014-10-01

    Full Text Available In the present paper, testing methods currently adopted to measure the in service ballast resistance are synthetically reviewed to identify the main sources of uncertainty influencing the test loads and to define an experimental methodology allowing the optimal control of the testing parameters without the introduction of spurious or parasitic actions on the track sample. An alternative testing system, which allows applying on a fullscale sample of a railway track testing loads very close the real ones, is presented. Of the new system, both the ways of use for measuring the transversal and axial ballast strength, the general procedure to carry out the experimentation and its application to a real scenario are described, highlighting its main advantages in terms both of modalities for applying the loads and of testing parameter control.

  5. Effects of striated laser tracks on thermal fatigue resistance of cast iron samples with biomimetic non-smooth surface

    International Nuclear Information System (INIS)

    Tong, Xin; Zhou, Hong; Liu, Min; Dai, Ming-jiang

    2011-01-01

    In order to enhance the thermal fatigue resistance of cast iron materials, the samples with biomimetic non-smooth surface were processed by Neodymium:Yttrium Aluminum Garnet (Nd:YAG) laser. With self-controlled thermal fatigue test method, the thermal fatigue resistance of smooth and non-smooth samples was investigated. The effects of striated laser tracks on thermal fatigue resistance were also studied. The results indicated that biomimetic non-smooth surface was benefit for improving thermal fatigue resistance of cast iron sample. The striated non-smooth units formed by laser tracks which were vertical with thermal cracks had the best propagation resistance. The mechanisms behind these influences were discussed, and some schematic drawings were introduced to describe them.

  6. Multidrug resistant Vibrio cholerae O1 from clinical and environmental samples in Kathmandu city.

    Science.gov (United States)

    Thapa Shrestha, Upendra; Adhikari, Nabaraj; Maharjan, Rojina; Banjara, Megha R; Rijal, Komal R; Basnyat, Shital R; Agrawal, Vishwanath P

    2015-02-27

    Cholera, an infectious disease caused by Vibrio cholerae, is a major public health problem and is a particularly burden in developing countries including Nepal. Although the recent worldwide outbreaks of cholera have been due to V. cholerae El Tor, the classical biotypes are still predominant in Nepal. Serogroup O1 of the V. cholerae classical biotype was the primary cause of a cholera outbreak in Kathmandu in 2012. Thus, this study was designed to know serotypes and biotypes of V. cholerae strains causing recent outbreak with reference to drug resistant patterns. Moreover, we also report the toxigenic strains of V. cholerae from both environmental and clinical specimens by detecting the ctx gene. Twenty four V. cholerae (n = 22 from stool samples and n = 2 from water samples) isolated in this study were subjected to Serotyping and biotyping following the standard protocols as described previously. All of the isolates were tested for antimicrobial susceptibility patterns using the modified Kirby-Bauer disk diffusion method as recommended by CLSI guidelines. The screening of the ctx genes (ctxA2-B gene) were performed by PCR method using a pair of primers; C2F (5'-AGGTGTAAAATTCCTTGACGA-3') and C2R (5'-TCCTCAGGGTATCCTTCATC-3') to identify the toxigenic strains of V. cholerae. Among twenty four V. cholerae isolates, 91.7% were clinical and 8.3% were from water samples. Higher rate of V. cholerae infection was found among adults of aged group 20-30 years. All isolates were serogroups O1 of the V. cholerae classical biotype and sub serotype, Ogawa. All isolates were resistant to ampicillin, nalidixic acid and cotrimoxazole. 90.9% were resistant to erythromycin however, tetracycline was found to be the most effective drug for the isolates. All isolates were multidrug resistant (MDR) and possessed a ctx gene of approximately 400 base pairs indicating the toxigenic strains. Hundred percent strains of V. cholerae were MDR possessing a ctx gene. It suggests that

  7. Genetic Lineages and Antimicrobial Resistance in Pseudomonas spp. Isolates Recovered from Food Samples.

    Science.gov (United States)

    Estepa, Vanesa; Rojo-Bezares, Beatriz; Torres, Carmen; Sáenz, Yolanda

    2015-06-01

    Raw food is a reservoir of Pseudomonas isolates that could be disseminated to consumers. The presence of Pseudomonas spp. was studied in food samples, and the phenotypic and genotypic characterizations of the recovered isolates were analyzed. Two samples of meat (3%, turkey and beef) and 13 of vegetables (22%, 7 green peppers and 6 tomatoes) contained Pseudomonas spp. A total of 20 isolates were identified, and were classified as follows (number of isolates): P. aeruginosa (5), P. putida (5), P. nitroreducens (4), P. fulva (2), P. mosselli (1), P. mendocina (1), P. monteilii (1), and Pseudomonas sp. (1). These 20 Pseudomonas isolates were clonally different by pulsed-field-gel-electrophoresis, and were resistant to the following antibiotics: ticarcillin (85%), aztreonam (30%), cefepime (10%), imipenem (10%), and meropenem (5%), but were susceptible to ceftazidime, piperacillin, piperacillin-tazobactam, doripenem, gentamicin, tobramycin, amikacin, ciprofloxacin, norfloxacin, and colistin. Only one strain (Ps158) presented a class 1 integron lacking the 3' conserved segment. The five P. aeruginosa strains were typed by multilocus sequence typing in five different sequence-types (ST17, ST270, ST800, ST1455, and ST1456), and different mutations were detected in protein OprD that were classified in three groups. One strain (Ps159) showed a new insertion sequence (ISPa47) truncating the oprD gene, and conferring resistance to imipenem.

  8. Dipole-Dipole Resistivity Measurements of Weathered Soils Over ...

    African Journals Online (AJOL)

    These were characterized by the H, A, Q and K- type curves which corresponded to the resistivity sequence r1>r2r2>r3, r1r3 respectively. Over the Birimian rocks, near surface resistiv-ity values ranged from100-600 ohm-m within the depths from 0-9 m. A moderately resistive stra-tum with values ranging from 200-500 ...

  9. High resolution resistivity measurements at the Down Ampney research site

    International Nuclear Information System (INIS)

    Hallam, J.R.; Jackson, P.D.; Rainsbury, M.; Raines, M.

    1991-01-01

    A new high resolution resistivity surveying method is described for fault detection and characterisation. The resolution is shown to be significantly higher than conventional apparent resistivity profiling when applied to geological discontinuities such as faults. Nominal fault locations have been determined to an accuracy of 0.5 m, as proven by drilling. Two dimensional profiling and image enhancement of the resulting 2-D data set indicated the possibility of subsidiary fractures and/or lateral changes within the clay to clay' fault zone. The increased resolution allows greater confidence to be placed on both the fault detection and lateral perturbations derived from processed resistance and resistivity images. (Author)

  10. Calorimetric method for current sharing temperature measurements in ITER conductor samples in SULTAN

    International Nuclear Information System (INIS)

    Bagnasco, M.

    2009-01-01

    Several Toroidal Field Conductor short samples with slight layout variations have been assembled and tested in the SULTAN facility at CRPP. The measurement campaigns started in 2007 and are still ongoing. The performance of every conductor is expressed in terms of current sharing temperature (T cs ), i.e. the temperature at which a defined electric field, 10 μV/m, is detected in the cable due to the incipient superconducting-to-normal state transition. The T cs at specific operating conditions is the key design parameter for the ITER conductors and is the main object of the qualification tests. Typically, the average electric field is measured with voltage tap pairs attached on the jacket along the conductor. The inability however to explain observed premature voltage developments opened the discussion about possible alternative measuring methods. The He flow calorimetric method is based on the measurement of the resistive power generation in the conductor. It relies on the detection of very small temperature increases along the conductor in steady state operation. The accuracy and the reliability of the calorimetric method in SULTAN are critically discussed, with particular emphasis on the instrumentation requirements and test procedures. The application of the calorimetric method to the recent SULTAN test campaigns is described with its merits and limits. For future tests of ITER conductors in SULTAN, the calorimetric method for T cs test is proposed as a routine procedure.

  11. Measurement of thermal properties of soil and concrete samples

    DEFF Research Database (Denmark)

    Pagola, Maria Alberdi; Jensen, Rasmus Lund; Madsen, Søren

    This document aims to present the laboratory work undertaken to analyse the thermal properties of the soil at two test sites in Denmark and the concrete produced by Centrum Pæle A/S, used to produce the pile heat exchangers studied in the present PhD project. The tasks have been carried out between...... “Thermal response testing of precast pile heat exchangers: fieldwork report” by (Alberdi-Pagola et al., 2017). The report is organized as follows: first, the measurement methods and the test procedures are described. Second, the soils at both test sites are described, together with the measurements. Third......, the measurements of the properties of the concrete are treated. The work is extended in appendixes....

  12. Miniature nanoparticle sensors for exposure measurement and TEM sampling

    International Nuclear Information System (INIS)

    Fierz, Martin; Meier, Dominik; Steigmeier, Peter; Burtscher, Heinz

    2015-01-01

    Nanoparticles in workplaces may pose a threat to the health of the workers involved. With the general boom in nanotechnology, an increasing number of workers is potentially exposed, and therefore a comprehensive risk management with respect to nanoparticles appears necessary. One (of many) components of such a risk management is the measurement of personal exposure. Traditional nanoparticle detectors are often cumbersome to use, large, heavy and expensive. We have developed small, reliable and easy to use devices that can be used for routine personal exposure measurement in workplaces. (paper)

  13. Intercomparison of radionuclide measurements in marine sediment sample IAEA-368

    International Nuclear Information System (INIS)

    Ballestra, S.; Lopez, J.J.; Gastaud, J.; Parsi, P.; Vas, D.; Noshkin, V.

    1991-08-01

    The results of an intercomparison exercise on a Pacific Ocean sediment sample, IAEA-368, designed for the determination of artificial and natural radionuclides levels, are reported. The data from 89 laboratories representing 37 countries have been evaluated. The following are the recommended values, with confidence intervals, for 60 Co, 155 Eu, 210 7Pb, 226 Ra, 238 U, 238 Pu and 239+240 Pu (Reference date: 1 January 1990). Information values for 40 K, 90 Sr, 137 Cs, 228 Th, 230 Th, 232 Th, 234 U, 235 U and 241 Am are also reported. (author)

  14. Intercomparison of radionuclide measurements in marine sediment sample IAEA-367

    International Nuclear Information System (INIS)

    Ballestra, S.; Lopez, J.J.; Gastaud, J.; Vas, D.; Noshkin, V.

    1991-08-01

    The results of an intercomparison exercise on a Pacific Ocean sediment sample, IAEA-367, designed for the determination of artificial and natural radionuclides levels, are reported. The data from 81 laboratories representing 37 countries have been evaluated. The following are the recommended values, with confidence intervals, for 60 Co, 90 Sr, 137 Cs, 239+240 Pu (Reference date: 1 January 1990). Information values for 155 Eu, 238 Pu, 241 Am, and 241 Pu are reported. Information values for the following natural radionuclides 40 K, 226 Ra, 228 Th, 230 Th, 234 U, 235 U and 238 U are also reported. (author)

  15. Measuring Thermal Conductivity of a Small Insulation Sample

    Science.gov (United States)

    Miller, Robert A.; Kuczmarski, Maria A.

    2009-01-01

    A multiple-throat venturi system has been invented for measuring laminar flow of air or other gas at low speed (1 to 30 cm/s) in a duct while preserving the laminar nature of the flow and keeping the velocity profile across the duct as nearly flat as possible. While means for measuring flows at higher speeds are well established, heretofore, there have been no reliable means for making consistent, accurate measurements in this speed range. In the original application for which this system was invented, the duct leads into the test section of a low-speed wind tunnel wherein uniform, low-speed, laminar flow is required for scientific experiments. The system could also be used to monitor a slow flow of gas in an industrial process like chemical vapor deposition. In the original application, the multiple- throat venturi system is mounted at the inlet end of the duct having a rectangular cross section of 19 by 14 cm, just upstream of an assembly of inlet screens and flow straighteners that help to suppress undesired flow fluctuations (see Figure 1). The basic venturi measurement principle is well established: One measures the difference in pressure between (1) a point just outside the inlet, where the pressure is highest and the kinetic energy lowest; and (2) the narrowest part (the throat) of the venturi passage, where the kinetic energy is highest and the pressure is lowest. Then by use of Bernoulli s equation for the relationship between pressure and kinetic energy, the volumetric flow speed in the duct can be calculated from the pressure difference and the inlet and throat widths. The design of this system represents a compromise among length, pressure recovery, uniformity of flow, and complexity of assembly. Traditionally, venturis are used to measure faster flows in narrower cross sections, with longer upstream and downstream passages to maintain accuracy. The dimensions of the passages of the present venturi system are sized to provide a readily measurable

  16. Characterization of antimicrobial resistance and quinolone resistance factors in high-level ciprofloxacin-resistant Enterococcus faecalis and Enterococcus faecium isolates obtained from fresh produce and fecal samples of patients.

    Science.gov (United States)

    Kim, Min-Chan; Woo, Gun-Jo

    2017-07-01

    The emergence of fluoroquinolone-resistant enterococci is worldwide. Antimicrobial resistance was characterized and the effect of quinolone-resistance factors was analyzed in high-level ciprofloxacin-resistant (HLCR) Enterococcus faecalis and Enterococcus faecium isolated from fresh produce and fecal samples of patients. Among the 81 ciprofloxacin-resistant Enterococcus isolates, 46 showed high levels of ciprofloxacin resistance, resistance to other quinolone antibiotics, and multidrug resistance profiles. The virulence factors esp and hyl were identified in 27 (58.7%) and 25 (54.3%) of isolates, respectively. Sequence type analysis showed that 35 strains of HLCR E. faecium were clonal complex 17. Eleven strains of HLCR E. faecalis were confirmed as sequence type (ST) 28, ST 64 and ST 125. Quinolone resistance-determining region mutation was identified in HLCR Enterococcus isolates; with serine being changed in gyrA83, gyrA87 and parC80. This result shows that gyrA and parC mutations could be important factors for high-level resistance to fluoroquinolones. No significant differences were observed in antimicrobial resistance patterns and genetic characteristics among the isolates from fresh produce and fecal samples. Therefore, good agricultural practices in farming and continuous monitoring of patients, food and the environment for Enterococcus spp. should be performed to prevent antimicrobial resistance and enable reduction of resistance rates. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  17. Antibiotic resistance development and identification of response measures

    NARCIS (Netherlands)

    ter Kuile, B.; Brul, S.; Sofos, J.

    2013-01-01

    Antibiotic resistance in human pathogens is becoming a major threat to human health. This is partly due to agriculture, because large amounts of antibiotics are used to treat animals, which encourages resistant genes to appear and transfer to humans through foodstuffs. This is a food safety issue.

  18. Prevalence of Streptococcus spp. collected from vaginal samples and their antibiotic resistance

    Directory of Open Access Journals (Sweden)

    Carmela Mazzone

    2010-09-01

    Full Text Available Background. Streptococci are a heterogeneous group Gram positive bacteria, predominantly anaerobes. They are classified by growth characteristics, hemolysis on blood and antigenic composition.Among the most pathogenic streptococci are ß-hemolytic Streptococcus group B (GBS responsible for pneumonia, meningitis and sepsis in newborn. In order to prevent infections that might involve not only the female but also but also the future newborn, a bacterial culture have to be performed during pregnancy. Objectives. This study aims to assess the prevalence of GBS and their resistance to antibiotics among bacteria collected from vaginal samples. Methods. During the considered period of study: May 2008 - May 2009, 384 vaginal samples from young women were microbiologically investigated. Swabs were sown on CNA (bioMérieux plates and incubated under CO2 environment for 24-48 hours. For the identification of group and the evaluation of their susceptibility to antibiotics the kit Slidex streptococcal Plus (bioMérieux and the ATB Strep (bioMéieux respectively were used. Breakpoint values were as suggested by CLSI. Results. GBS was the most frequently pathogen found with 59.5% (69 on a total of 116 positive samples, followed by Group D streptococci with 34.5% (40, and Group A with 4.3% (5, Group C and F, accounted for 0.8% (1. Moreover, all the isolates, with the exception of group D, resulted susceptible to penicillin, amoxicillin, vancomycin and cefotaxime, 45% to erythromycin, 47% to clindamycin, 75% to cotrimoxazole. Conclusions. The data from this survey show that GBS is the prevalent pathogen, among Streptococcus spp collected from the vagina.The percentage of antibiotic resistant strains found confirms the importance of the Clinical Pathology Laboratory in the surveillance of streptococcal infections for the control and prevention.

  19. Virulence and antimicrobial resistance of Enterococcus faecium isolated from water samples.

    Science.gov (United States)

    Enayati, M; Sadeghi, J; Nahaei, M R; Aghazadeh, M; Pourshafie, M R; Talebi, M

    2015-10-01

    The aim of this study was to determine the incidence of Enterococcus species and six virulence factors of Enterococcus faecium which were isolated from surface water and wells. Fifteen different water samples, which were used for drinking as well as agricultural irrigation, were collected from nine private wells and surface water from six rivers located at the east of Tehran. The Ent. faecium isolates were tested for their resistance to 10 antibiotics and their virulence factors were detected using multiplex PCR for esp, acm, gelE, asa1, cylA and hyl genes. The most predominant species in 315 isolates were Ent. faecium (n = 118) followed by Enterococcus galinarom (n = 110), Enterococcus mundeti (n = 18), Enterococcus hirea (n = 37) and Enterococcus casselifelavus (n = 32). The resistance rates were observed in 41·5, 27·1, 12·7, 6·8 and 1·7% isolates for tetracycline, erythromycin, ampicillin, ciprofloxacin and chloramphenicol respectively. None of the Ent. faecium isolates were resistant to vancomycin, teicoplanin, linezolid, gentamicin and quinuspristin-dalfopristin. Virulence determinant was found in 84·7, 33·9, 16·1 and 2·5% of isolates for acm, asa1, esp, cylA respectively. None of the isolates carried hyl and gelE gene. The presence of virulence factors and antibiotic resistance indicated that water might be an important source of dissemination of virulent enterococci. Contamination of drinking or recreational water by human or animal faecal waste is a major public health threat. In this study, we determine the incidence of Enterococcus species and six virulence factors of Enterococcus faecium which were isolated from surface water and wells. Results from this study suggest that the presence of Ent. faecium in natural and well waters was found to be significant in rural areas of Tehran. Resistant to erythromycin among Ent. faecium was relatively high and the incidence of acm and asa1 among our isolates was common overall. © 2015 The

  20. Magnetoacoustic measurements on steel samples at low magnetizing frequencies

    Czech Academy of Sciences Publication Activity Database

    Perevertov, Oleksiy; Stupakov, Alexandr

    2015-01-01

    Roč. 66, č. 7 (2015), s. 58-61 ISSN 1335-3632 R&D Projects: GA ČR GA13-18993S; GA ČR GB14-36566G Institutional support: RVO:68378271 Keywords : magneto-acoustic emission * surface magnetic field * steel * magnetic hysteresis Subject RIV: JB - Sensors, Measurment, Regulation Impact factor: 0.407, year: 2015

  1. An Investigation of Antibiotic Resistance Pattern in the Strains of Methicillin-resistant Staphylococcus epidermidis Isolated From Clinical Samples in Isfahan Province, Iran

    Directory of Open Access Journals (Sweden)

    Fahimeh Nourbakhsh

    2016-08-01

    Full Text Available Background and Objectives: Staphylococcus epidermidis is one of the effective factors causing nosocomial infections. This study was performed to investigate the antibiotic resistance pattern in the methicillin-resistant S. epidermidis strains isolated from clinical samples in Isfahan Province. Methods: In this descriptive cross-sectional study, 150 isolates of S. epidermidis were isolated from detected from the patients hospitalized in hospitals and treatment centers of Isfahan City. The antibiotic resistance pattern was evaluated by disk diffusion method. The presence of the gene encoding antibiotic resistance to methicillin (mec A in the isolates were investigated using PCR method. Data were analyzed with Chi-square and Fisher's exact statistical tests. Results: In this study, most isolates were related to urinary tract infections. The highest resistance was reported to penicillin (98.9%, erythromycin (89.4%, ciprofloxacin (77.7%, clindamycin (65.9%, tetracycline (63.2%, and meticillin (54%. None of the strains showed resistance to vancomycin and linezolid. Molecular studies indicated the presence of mecA gene in 76% of the studied isolates. Conclusion: According to the results of this study, vancomycin and linezolid antibiotics can be the best choice of treatment for infections caused by S. epidermidis. Also, high resistance of S. epidermidis can be a serious warning for increased multiple antibiotic resistance. Molecular studies are indicative of high sensitivity of molecular methods in the investigation of methicillin-resistant isolates.  

  2. Precision rectifier detectors for ac resistance bridge measurements with application to temperature control systems for irradiation creep experiments

    Energy Technology Data Exchange (ETDEWEB)

    Duncan, M. G.

    1977-05-01

    The suitability of several temperature measurement schemes for an irradiation creep experiment is examined. It is found that the specimen resistance can be used to measure and control the sample temperature if compensated for resistance drift due to radiation and annealing effects. A modified Kelvin bridge is presented that allows compensation for resistance drift by periodically checking the sample resistance at a controlled ambient temperature. A new phase-insensitive method for detecting the bridge error signals is presented. The phase-insensitive detector is formed by averaging the magnitude of two bridge voltages. Although this method is substantially less sensitive to stray reactances in the bridge than conventional phase-sensitive detectors, it is sensitive to gain stability and linearity of the rectifier circuits. Accuracy limitations of rectifier circuits are examined both theoretically and experimentally in great detail. Both hand analyses and computer simulations of rectifier errors are presented. Finally, the design of a temperature control system based on sample resistance measurement is presented. The prototype is shown to control a 316 stainless steel sample to within a 0.15/sup 0/C short term (10 sec) and a 0.03/sup 0/C long term (10 min) standard deviation at temperatures between 150 and 700/sup 0/C. The phase-insensitive detector typically contributes less than 10 ppM peak resistance measurement error (0.04/sup 0/C at 700/sup 0/C for 316 stainless steel or 0.005/sup 0/C at 150/sup 0/C for zirconium).

  3. Influence of sampling points on inspection accuracy of free-form surfaces using coordinate measuring machine

    Science.gov (United States)

    Xie, Mengmin; Chen, Yueping; Zhang, Anshe; Fang, Rui

    2018-03-01

    The inspection accuracy of free-form surfaces is mainly affected by the processing, the number of sampling points, the distribution of sampling points, the measurement equipment and other factors. This paper focuses on the influence of sampling points on inspection accuracy of free-form surfaces, and isoparametric distribution was used in sample point distribution. Different sampling points number was compared on a same surface and a probe, the measurement data were analyzed and the optimal sampling points number was obtained.

  4. Measurement of phthalates in small samples of mammalian tissue

    International Nuclear Information System (INIS)

    Acott, P.D.; Murphy, M.G.; Ogborn, M.R.; Crocker, J.F.S.

    1987-01-01

    Di-(2-ethylhexyl)-phthalate (DEHP) is a phthalic acid ester that is used as a plasticizer in polyvinyl chloride products, many of which have widespread medical application. DEHP has been shown to be leached from products used for storage and delivery of blood transfusions during procedures such as plasmaphoresis, hemodialysis and open heart surgery. Results of studies in this laboratory have suggested that there is an association between the absorption and deposition of DEHP (and/or related chemicals) in the kidney and the acquired renal cystic disease (ACD) frequently seen in patients who have undergone prolonged dialysis treatment. In order to determine the relationship between the two, it has been necessary to establish a method for extracting and accurately quantitating minute amounts of these chemicals in small tissue samples. The authors have now established such a method using kidneys from normal rats and from a rat model for ACD

  5. Measurement of Irradiated Pyroprocessing Samples via Laser Induced Breakdown Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Phongikaroon, Supathorn [Virginia Commonwealth Univ., Richmond, VA (United States)

    2016-10-31

    The primary objective of this research is to develop an applied technology and provide an assessment to remotely measure and analyze the real time or near real time concentrations of used nuclear fuel (UNF) dissolute in electrorefiners. Here, Laser-Induced Breakdown Spectroscopy (LIBS), in UNF pyroprocessing facilities will be investigated. LIBS is an elemental analysis method, which is based on the emission from plasma generated by focusing a laser beam into the medium. This technology has been reported to be applicable in the media of solids, liquids (includes molten metals), and gases for detecting elements of special nuclear materials. The advantages of applying the technology for pyroprocessing facilities are: (i) Rapid real-time elemental analysis|one measurement/laser pulse, or average spectra from multiple laser pulses for greater accuracy in < 2 minutes; (ii) Direct detection of elements and impurities in the system with low detection limits|element specific, ranging from 2-1000 ppm for most elements; and (iii) Near non-destructive elemental analysis method (about 1 g material). One important challenge to overcome is achieving high-resolution spectral analysis to quantitatively analyze all important fission products and actinides. Another important challenge is related to accessibility of molten salt, which is heated in a heavily insulated, remotely operated furnace in a high radiation environment with an argon atmosphere.

  6. Antimicrobial resistance in faecal samples from buffalo, wildebeest and zebra grazing together with and without cattle in Tanzania.

    Science.gov (United States)

    Katakweba, A A S; Møller, K S; Muumba, J; Muhairwa, A P; Damborg, P; Rosenkrantz, J T; Minga, U M; Mtambo, M M A; Olsen, J E

    2015-04-01

    The aim of this study was to determine whether the practice of co-grazing with cattle and wild life constitutes a risk of transmission of antibiotic resistant bacteria to wild ungulates. Faecal samples were collected from buffalo (n = 35), wildebeest (n = 40), zebra (n = 40) and cattle (N = 20) from Mikumi National Park, Tanzania (MNP), where cattle is prohibited and from Ngorongoro Conservation Area (NCA) where co-grazing is practiced. The number of coliforms and enterococci resistant to selected antibiotics was determined. Wild life generally harboured higher number of resistant Escherichia coli and Enterococci than cattle, but with no general influence in wild life of co-grazing with cattle. Vancomycin-resistant Enterococci were detected in wild life samples, and E. coli resistant to cefotaxime and enrofloxacin were observed among isolates from all wild life, but not from cattle. Culture independent estimates of the number of sulII gene copies obtained by qPCR did not differ between wild life from the two sample sites, while tetW was significantly higher in samples from MPN than from NCA. Antibiotic resistant bacteria were not more frequently found in ungulates grazing together with cattle than ungulates without this interaction. This study did not indicate that transmission of antibiotic resistant bacteria is a frequent event following co-grazing of wild life and cattle. © 2014 The Society for Applied Microbiology.

  7. A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

    Directory of Open Access Journals (Sweden)

    Shivani Gupta

    2017-01-01

    Full Text Available The prevalence of drug resistance (DR mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV, supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research. Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR and reverse transcriptase (RT regions of HIV-1 pol gene across many HIV-1 subtypes from LLV plasma samples. HIV-spiked plasma samples from the External Quality Assurance Program Oversight Laboratory (EQAPOL, covering various HIV-1 subtypes, as well as clinical specimens were used to optimize and validate the protocol. Our results demonstrate that this protocol has a broad HIV-1 subtype coverage and viral load span with high sensitivity and reproducibility. Moreover, the protocol is robust even when plasma sample volumes are limited, the HIV viral load is unknown, and/or the HIV subtype is undetermined. Thus, the protocol is applicable for the initial amplification of the HIV-1 PR and RT genes required for subsequent genotypic DR assays.

  8. Measuring injury risk factors: question reliability in a statewide sample.

    Science.gov (United States)

    Koziol-McLain, J; Brand, D; Morgan, D; Leff, M; Lowenstein, S R

    2000-06-01

    Recently (1996-98), Colorado added 15 questions pertaining to injury related risks and behaviors to the behavioral risk factor surveillance system (BRFSS). Questions addressed bicycle helmet use, traffic crashes, exposure to violence, suicidal behavior, and gun storage. To measure the test-retest reliability of these injury related questions. Of 330 BRFSS participants, 229 (69%) were called a second time and reasked nine selected injury questions. Retests were completed 7-28 days after the original interview. Test-retest agreement was very high (kappa >0.80) for bicycle helmet use, domestic police visits, and gun ownership. All other injury risk questions had substantial agreement (kappa >0.60). The injury related questions added to the Colorado BRFSS have high test-retest reliability.

  9. Increased prevalence of antibiotic-resistant E. coli in gulls sampled in southcentral Alaska is associated with urban environments

    Science.gov (United States)

    Atterby, Clara; Ramey, Andrew M.; Gustafsson Hall, Gabriel; Jarhult, Josef; Borjesson, Stefan; Bonnedahl, Jonas

    2016-01-01

    BackgroundAntibiotic-resistant bacteria pose challenges to healthcare delivery systems globally; however, limited information is available regarding the prevalence and spread of such bacteria in the environment. The aim of this study was to compare the prevalence of antibiotic-resistant bacteria in large-bodied gulls (Larus spp.) at urban and remote locations in Southcentral Alaska to gain inference into the association between antibiotic resistance in wildlife and anthropogenically influenced habitats.MethodsEscherichia coli was cultured (n=115 isolates) from fecal samples of gulls (n=160) collected from a remote location, Middleton Island, and a more urban setting on the Kenai Peninsula.ResultsScreening of E. coli from fecal samples collected from glaucous-winged gulls (Larus glaucescens) at Middleton Island revealed 8% of isolates were resistant to one or more antibiotics and 2% of the isolates were resistant to three or more antibiotics. In contrast, 55% of E. coli isolates derived from fecal samples collected from large-bodied gulls (i.e. glaucous, herring [Larus argentatus], and potentially hybrid gulls) on the Kenai Peninsula were resistant to one or more antibiotics and 22% were resistant to three or more antibiotics. In addition, total of 16% of the gull samples from locations on the Kenai Peninsula harbored extended-spectrum cephalosporin-resistant E. coli isolates (extended-spectrum beta-lactamases [ESBL] and plasmid-encoded AmpC [pAmpC]), in contrast to Middleton Island where no ESBL- or pAmpC-producing isolates were detected.ConclusionOur findings indicate that increased prevalence of antibiotic resistance is associated with urban environments in Southcentral Alaska and presumably influenced by anthropogenic impacts. Further investigation is warranted to assess how migratory birds may maintain and spread antimicrobial-resistant bacteria of relevance to human and animal health.

  10. Correlation of Insulin Resistance with Anthropometric Measures and Blood Pressure in Adolescents

    Science.gov (United States)

    de Morais, Polyana Resende Silva; Sousa, Ana Luiza Lima; Jardim, Thiago de Souza Veiga; Nascente, Flávia Miquetichuc Nogueira; Mendonça, Karla Lorena; Povoa, Thaís Inácio Rolim; Carneiro, Carolina de Souza; Ferreira, Vanessa Roriz; de Souza, Weimar Kunz Sebba Barroso; Jardim, Paulo César Brandão Veiga

    2016-01-01

    Background Blood pressure is directly related to body mass index, and individuals with increased waist circumference have higher risk of developing hypertension, insulin resistance, and other metabolic changes, since adolescence. Objective to evaluate the correlation of blood pressure with insulin resistance, waist circumference and body mass index in adolescents. Methods Cross-section study on a representative sample of adolescent students. One group of adolescents with altered blood pressure detected by casual blood pressure and/or home blood pressure monitoring (blood pressure > 90th percentile) and one group of normotensive adolescents were studied. Body mass index, waist circumference were measured, and fasting glucose and plasma insulin levels were determined, using the HOMA-IR index to identify insulin resistance. Results A total of 162 adolescents (35 with normal blood pressure and 127 with altered blood pressure) were studied; 61% (n = 99) of them were boys and the mean age was 14.9 ± 1.62 years. Thirty-eight (23.5%) adolescents had altered HOMA-IR. The group with altered blood pressure had higher values of waist circumference, body mass index and HOMA-IR (pHOMA-IR than boys (pHOMA-IR in the group with altered blood pressure (ρ = 0.394; p HOMA-IR in both groups (ρ = 0.345; p HOMA-IR was as predictor of altered blood pressure (odds ratio - OR = 2.0; p = 0.001). Conclusion There was a significant association of insulin resistance with blood pressure and the impact of insulin resistance on blood pressure since childhood. The correlation and association between markers of cardiovascular diseases was more pronounced in adolescents with altered blood pressure, suggesting that primary prevention strategies for cardiovascular risk factors should be early implemented in childhood and adolescence. PMID:27007222

  11. Assessing the precision of a time-sampling-based study among GPs: balancing sample size and measurement frequency.

    Science.gov (United States)

    van Hassel, Daniël; van der Velden, Lud; de Bakker, Dinny; van der Hoek, Lucas; Batenburg, Ronald

    2017-12-04

    Our research is based on a technique for time sampling, an innovative method for measuring the working hours of Dutch general practitioners (GPs), which was deployed in an earlier study. In this study, 1051 GPs were questioned about their activities in real time by sending them one SMS text message every 3 h during 1 week. The required sample size for this study is important for health workforce planners to know if they want to apply this method to target groups who are hard to reach or if fewer resources are available. In this time-sampling method, however, standard power analyses is not sufficient for calculating the required sample size as this accounts only for sample fluctuation and not for the fluctuation of measurements taken from every participant. We investigated the impact of the number of participants and frequency of measurements per participant upon the confidence intervals (CIs) for the hours worked per week. Statistical analyses of the time-use data we obtained from GPs were performed. Ninety-five percent CIs were calculated, using equations and simulation techniques, for various different numbers of GPs included in the dataset and for various frequencies of measurements per participant. Our results showed that the one-tailed CI, including sample and measurement fluctuation, decreased from 21 until 3 h between one and 50 GPs. As a result of the formulas to calculate CIs, the increase of the precision continued and was lower with the same additional number of GPs. Likewise, the analyses showed how the number of participants required decreased if more measurements per participant were taken. For example, one measurement per 3-h time slot during the week requires 300 GPs to achieve a CI of 1 h, while one measurement per hour requires 100 GPs to obtain the same result. The sample size needed for time-use research based on a time-sampling technique depends on the design and aim of the study. In this paper, we showed how the precision of the

  12. Rapid assessment of antimicrobial resistance prevalence using a Lot Quality Assurance sampling approach.

    Science.gov (United States)

    van Leth, Frank; den Heijer, Casper; Beerepoot, Mariëlle; Stobberingh, Ellen; Geerlings, Suzanne; Schultsz, Constance

    2017-04-01

    Increasing antimicrobial resistance (AMR) requires rapid surveillance tools, such as Lot Quality Assurance Sampling (LQAS). LQAS classifies AMR as high or low based on set parameters. We compared classifications with the underlying true AMR prevalence using data on 1335 Escherichia coli isolates from surveys of community-acquired urinary tract infection in women, by assessing operating curves, sensitivity and specificity. Sensitivity and specificity of any set of LQAS parameters was above 99% and between 79 and 90%, respectively. Operating curves showed high concordance of the LQAS classification with true AMR prevalence estimates. LQAS-based AMR surveillance is a feasible approach that provides timely and locally relevant estimates, and the necessary information to formulate and evaluate guidelines for empirical treatment.

  13. Biphasic DC measurement approach for enhanced measurement stability and multi-channel sampling of self-sensing multi-functional structural materials doped with carbon-based additives

    Science.gov (United States)

    Downey, Austin; D'Alessandro, Antonella; Ubertini, Filippo; Laflamme, Simon; Geiger, Randall

    2017-06-01

    Investigation of multi-functional carbon-based self-sensing structural materials for structural health monitoring applications is a topic of growing interest. These materials are self-sensing in the sense that they can provide measurable electrical outputs corresponding to physical changes such as strain or induced damage. Nevertheless, the development of an appropriate measurement technique for such materials is yet to be achieved, as many results in the literature suggest that these materials exhibit a drift in their output when measured with direct current (DC) methods. In most of the cases, the electrical output is a resistance and the reported drift is an increase in resistance from the time the measurement starts due to material polarization. Alternating current methods seem more appropriate at eliminating the time drift. However, published results show they are not immune to drift. Moreover, the use of multiple impedance measurement devices (LCR meters) does not allow for the simultaneous multi-channel sampling of multi-sectioned self-sensing materials due to signal crosstalk. The capability to simultaneously monitor multiple sections of self-sensing structural materials is needed to deploy these multi-functional materials for structural health monitoring. Here, a biphasic DC measurement approach with a periodic measure/discharge cycle in the form of a square wave sensing current is used to provide consistent, stable resistance measurements for self-sensing structural materials. DC measurements are made during the measurement region of the square wave while material depolarization is obtained during the discharge region of the periodic signal. The proposed technique is experimentally shown to remove the signal drift in a carbon-based self-sensing cementitious material while providing simultaneous multi-channel measurements of a multi-sectioned self-sensing material. The application of the proposed electrical measurement technique appears promising for real

  14. Surface resistance measurement of superconducting YBa sub 2 Cu sub 3 O sub 7 in a magnetic field

    Energy Technology Data Exchange (ETDEWEB)

    Chin, C.C. (Center for Materials Science and Engineering, Massachusetts Institute of Technology, Department of Physics, Massachusetts Institute of Technology, Cambridge, MA (USA)); Rainville, P.J.; Drehman, A.J.; Derov, J.S.; Steinbeck, J. (Rome Air Development Center (RADC), Hanscom AFB, MA (USA)); Dresselhaus, G. (Francis Bitter National Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, MA (USA)); Dresselhaus, M.S. (Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA (USA))

    1990-08-01

    We report on the magnetic-field dependent surface resistance of polycrystalline YBa{sub 2}Cu{sub 3}O{sub 7} ({ital T}{sub {ital c}}{congruent}92 K), measured using a brass cylindrical cavity resonator, operating at 16.5 GHz in the TE{sub 011} mode. A dc magnetic field {ital H}{sub app} is applied parallel to the superconducting sample surface, and the temperature dependence of the surface resistance is measured for four different values of {ital H}{sub app} (0 T, 0.22 T, 1 T, 5 T). An effective medium theory and the two-fluid model are used to fit the surface resistance versus temperature measurements both in zero field and for various applied fields. These results are applied to characterize the microwave properties of a polycrystalline ceramic superdconductor.

  15. Measuring changes in aerodynamic/rolling resistances by cycle-mounted power meters.

    Science.gov (United States)

    Lim, Allen C; Homestead, Eric P; Edwards, Andrew G; Carver, Todd C; Kram, Rodger; Byrnes, William C

    2011-05-01

    To develop a protocol for isolating changes in aerodynamic and rolling resistances from field-based measures of power and velocity during level bicycling. We assessed the effect of body position (hands on brake hoods vs drops) and tire pressure changes (414 vs 828 kPa) on aerodynamic and rolling resistances by measuring the power (Pext)-versus-speed (V) relationship using commercially available bicycle-mounted power meters. Measurements were obtained using standard road bicycles in calm wind (Aerodynamic resistance per velocity squared (k) was calculated as the slope of a linear plot of tractive resistance (RT=power/velocity) versus velocity squared. Rolling resistance (Rr) was calculated as the intercept of this relationship. Aerodynamic resistance per velocity squared (k) was significantly greater (Paerodynamic and rolling resistances associated with modest changes in body position and substantial changes in tire pressure. © 2011 by the American College of Sports Medicine

  16. Sampling artifacts in measurement of elemental and organic carbon: Low-volume sampling in indoor and outdoor environments

    Science.gov (United States)

    Olson, David A.; Norris, Gary A.

    Experiments were completed to determine the extent of artifacts from sampling elemental carbon (EC) and organic carbon (OC) under sample conditions consistent with personal sampling. Two different types of experiments were completed; the first examined possible artifacts from oils used in personal environmental monitor (PEM) impactor plates, and the second examined artifacts from microenvironmental sampling using different sampling media combinations (quartz, Teflon, XAD denuder, and electrostatic precipitator). The effectiveness of front and backup filters was evaluated for most sampling configurations. Mean total carbon concentrations from sampling configurations using impactor oils were not statistically different from the control case (using a sharp cut cyclone). Three microenvironments were tested (kitchen, library, and ambient); carbon concentrations were highest in the kitchen using a front quartz filter (mean OC of 16.4 μg m -3). The lowest front quartz filter concentrations were measured in the library using XAD denuders (mean OC of 3.6 μg m -3). Denuder removal efficiencies (average of 82% for total carbon) were lower compared with previous ambient studies and may indicate that indoor sources influenced denuder efficiency during sample collection. The highest carbon concentrations from backup quartz filters were measured using the Teflon-quartz combination.

  17. Prevalence and antibiotic resistance of Pseudomonas aeruginosa in water samples in central Italy and molecular characterization of oprD in imipenem resistant isolates.

    Directory of Open Access Journals (Sweden)

    Giuditta Fiorella Schiavano

    Full Text Available This study aimed to analyse the prevalence, antibiotic resistance and genetic relatedness of P. aeruginosa isolates obtained from potable and recreational water samples (n. 8,351 collected from different settings (swimming pools, n. 207; healthcare facilities, n 1,684; accommodation facilities, n. 1,518; municipal waterworks, n. 4,500; residential buildings, n. 235. Possible mechanisms underlying resistance to imipenem, with particular focus on those involving oprD-based uptake, were also explored.Isolation and identification of Pseudomonas aeruginosa was performed according to the standardized procedure UNI EN ISO 16266:2008 followed by PCR confirmation. Antibiotic Susceptibility testing was conducted according to EUCAST standardized disk diffusion method. Genetic relatedness of strains was carried out by RAPD. The sequence of the oprD gene was analyzed by standard method. Fifty-three samples (0.63% were positive for P. aeruginosa, of which 10/207 (4.83% were from swimming pools. Five isolates (9.43% were resistant to imipenem, one to Ticarcillin + Clavulanate, one to both Piperacillin and Ticarcillin + Clavulanate. The highest isolation rate of imipenem resistant P. aeruginosa was observed in swimming pool water. Identical RAPD profiles were found in isolates from the same location in the same year or even in different years.Imipenem resistant strains were identified as carbapenemase-negative and resistance has been associated with inactivating mutations within the oprD gene, with a concomitant loss of porin. RAPD results proved that a water system can remain colonized by one strain for long periods and the contamination may be difficult to eradicate. This study has revealed the presence of P. aeruginosa in different water samples, including resistant strains, especially in swimming pools, and confirmed the role of porins as a contributing factor in carbapenem resistance in Gram-negative bacteria.

  18. Effects of blood sample handling procedures on measurable inflammatory markers in plasma, serum and dried blood spot samples

    DEFF Research Database (Denmark)

    Skogstrand, K.; Thorsen, P.; Vogel, I.

    2008-01-01

    , and 30 days at the same temperatures. 27 inflammatory markers in serum and plasma and 25 markers in DBSS were measured by a previously validated multiplex sandwich immunoassay using Luminex xMAP technology. The measurable concentrations of several cytokines in serum and plasma were significantly......The interests in monitoring inflammation by immunoassay determination of blood inflammatory markers call for information on the stability of these markers in relation to the handling of blood samples. The increasing use of stored biobank samples for such ventures that may have been collected...... increased when blood samples were stored for a period of time before the centrifugation, for certain cytokines more than 1000 fold compared to serum and plasma isolated and frozen immediately after venepuncture. The concentrations in serum generally increased more than in plasma. The measurable...

  19. Effect of surface fissure on apparent resistivity measurements

    Science.gov (United States)

    Sailhac, P.; Gance, J.; Malet, J.

    2013-12-01

    Fissures are features of interest, prone to create preferential flow path, modifying locally the soil hydrogeological behavior. Electrical Resistivity Tomography (ERT) is a suitable tool to monitor such preferential flow path. However, this technique is not efficient in the presence of surface fissure, due to a bad resistivity recovering around the fissure vicinity during the inversion process. Therefore, we propose a description of fissure effect on raw apparent resistivity on three resistivity arrays. The purposes of the study are multiple. First, we aim at making ERT users aware of surface fissure effect, and propose a first help to interpret basically resistivity pseudo sections. Second, we propose to ERT users to automatically conduct a surface fissure survey on the studied profile, in order to consider each fissure in a forward DC model and to suppress their effect. Finally, this study is only a first step toward 2D fissure shape inversion, and time-lapse monitoring of fissure drying and filling. In this study, we create a fissure model based on different geomorphological descriptors. After describing the FEM-DC forward modeling strategy, we investigate the fissure effect on pseudo section of apparent resistivity for a Wenner-Schlumberger (WS), a dipole-dipole (DD) and a gradient (GRAD) array. We determine a fissure detectability threshold for each array and perform a sensitivity analysis on the different fissure parameters (position, width, depth, dip angles...). The crack filling or drying effect is also investigated. The possibility to remove fissure effect and to propose a first interpretation of time-lapse data is illustrated on real data. This study show again the higher sensitivity of the DD array compared to the GRAD and WS arrays. Not only the maximal amplitude in the pseudo section is higher for the DD array, but also the anomaly pattern created by the fissure is much larger for this acquisition geometry. The minimal depth detectable for the DD

  20. Effects of blood sample handling procedures on measurable inflammatory markers in plasma, serum and dried blood spot samples

    DEFF Research Database (Denmark)

    Skogstrand, K.; Thorsen, P.; Vogel, I.

    2008-01-01

    increased when blood samples were stored for a period of time before the centrifugation, for certain cytokines more than 1000 fold compared to serum and plasma isolated and frozen immediately after venepuncture. The concentrations in serum generally increased more than in plasma. The measurable......The interests in monitoring inflammation by immunoassay determination of blood inflammatory markers call for information on the stability of these markers in relation to the handling of blood samples. The increasing use of stored biobank samples for such ventures that may have been collected...... and stored for other purposes, justifies the study hereof. Blood samples were stored for 0, 4, 24, and 48 h at 4 degrees C, room temperature (RT), and at 35 degrees C, respectively, before they were separated into serum or plasma and frozen. Dried blood spot samples (DBSS) were stored for 0, 1, 2, 3, 7...

  1. Sample preparation method for ICP-MS measurement of 99Tc in a large amount of environmental samples

    International Nuclear Information System (INIS)

    Kondo, M.; Seki, R.

    2002-01-01

    Sample preparation for measurement of 99 Tc in a large amount of soil and water samples by ICP-MS has been developed using 95m Tc as a yield tracer. This method is based on the conventional method for a small amount of soil samples using incineration, acid digestion, extraction chromatography (TEVA resin) and ICP-MS measurement. Preliminary concentration of Tc has been introduced by co-precipitation with ferric oxide. The matrix materials in a large amount of samples were more sufficiently removed with keeping the high recovery of Tc than previous method. The recovery of Tc was 70-80% for 100 g soil samples and 60-70% for 500 g of soil and 500 L of water samples. The detection limit of this method was evaluated as 0.054 mBq/kg in 500 g soil and 0.032 μBq/L in 500 L water. The determined value of 99 Tc in the IAEA-375 (soil sample collected near the Chernobyl Nuclear Reactor) was 0.25 ± 0.02 Bq/kg. (author)

  2. Comparison of air samples, nasal swabs, ear-skin swabs and environmental dust samples for detection of Methicillin Resistant Staphylococcus aureus (MRSA) in pig herds

    DEFF Research Database (Denmark)

    Agersø, Yvonne; Vigre, Håkan; Cavaco, Lina

    2014-01-01

    To identify a cost-effective and practical method for detection of methicillin-resistant Staphylococcus aureus (MRSA) in pig herds, the relative sensitivity of four sample types: nasal swabs, ear-skin (skin behind the ears) swabs, environmental dust swabs and air was compared. Moreover, dependency......-herd prevalence ⩾25%]. The results indicate that taking swabs of skin behind the ears (ten pools of five) was even more sensitive than taking nasal swabs (ten pools of five) at the herd level and detected significantly more positive samples. spa types t011, t034 and t4208 were observed. In conclusion, MRSA...... detection by air sampling is easy to perform, reduces costs and analytical time compared to existing methods, and is recommended for initial testing of herds. Ear-skin swab sampling may be more sensitive for MRSA detection than air sampling or nasal swab sampling....

  3. Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Mohamed A. El-Tayeb

    Full Text Available Abstract Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39.4%, followed by S. Paratyphi (21.2%, S. Typhimurium (15.2%, S. Typhi and S. Arizona (12.1%, respectively. Most isolates were resistant to 1st and 2nd generation cephalosporin; and aminoglycosides. Moreover, several S. enterica isolates exhibited resistance to the first-line antibiotics used for Salmonellosis treatment including ampicillin, trimethoprim-sulfamethoxazole and chloramphenicol. In addition, the results revealed the emergence of two S. enterica isolates showing resistance to third-generation cephalosporin. Analysis of resistance determinants in S. enterica strains (n = 33 revealed that the resistance to β-lactam antibiotics, trimethoprim-sulfamethoxazole, chloramphenicol, and tetracycline, was attributed to the presence of carb-like, dfrA1, floR, tetA gene, respectively. On the other hand, fluoroquinolone resistance was related to the presence of mutations in gyrA and parC genes. These findings improve the information about foodborne Salmonella in Saudi Arabia, alarming the emergence of multi-drug resistant S. enterica strains, and provide useful data about the resistance mechanisms.

  4. Quality control for measurement of soil samples containing 237Np and 241Am as radiotracer

    International Nuclear Information System (INIS)

    Sha Lianmao; Zhang Caihong; Song Hailong; Ren Xiaona; Han Yuhu; Zhang Aiming; Chu Taiwei

    2003-01-01

    This paper reports quality control (QC) for the measurement of soil samples containing 237 Np and 241 Am as radiotracers in migration test of transuranic nuclides. All of the QC were done independently by the QA members of analytical work. It mainly included checking 5%-10% of the total analyzed samples; preparing blank samples, blind replicate sample and spiked samples used as quality control samples to check the quality of analytical work

  5. Simple and stable transendothelial electrical resistance measurement in organs-on-chips

    NARCIS (Netherlands)

    van der Helm, Marieke Willemijn; Odijk, Mathieu; Frimat, Jean-Philippe; Eijkel, Jan C.T.; van den Berg, Albert; Segerink, Loes Irene

    2015-01-01

    Measuring transendothelial electrical resistance (TEER) is a popular way to monitor cellular barrier tightness in organs-on-chips. However, in these devices integrated electrodes often block sight on the cells and the measured part often includes fluid-filled channels with variable resistance.

  6. 40 CFR 761.316 - Interpreting PCB concentration measurements resulting from this sampling scheme.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Interpreting PCB concentration... § 761.79(b)(3) § 761.316 Interpreting PCB concentration measurements resulting from this sampling... concentration measured in that sample. If the sample surface concentration is not equal to or lower than the...

  7. Evaluation of constitutive and inducible resistance to clindamycin in clinical samples of Staphylococcus aureus from a tertiary hospital

    Directory of Open Access Journals (Sweden)

    Angelita Bottega

    2014-10-01

    Full Text Available Introduction Infections caused by methicillin-resistant Staphylococcus aureus (MRSA have become common in hospitals and the community environment, and this wide resistance has limited patient treatment. Clindamycin (CL represents an important alternative therapy for infections caused by S. aureus. Antimicrobial susceptibility testing using standard methods may not detect inducible CL resistance. This study was performed to detect the phenotypes of resistance to macrolides-lincosamides-streptogramin B (MLSB antibiotics, including CL, in clinical samples of S. aureus from patients at a tertiary hospital in Santa Maria, State of Rio Grande do Sul, Brazil. Methods One hundred and forty clinical isolates were submitted to the disk diffusion induction test (D-test with an erythromycin (ER disk positioned at a distance of 20mm from a CL disk. The results were interpreted according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI. Results In this study, 29 (20.7% of the 140 S. aureus samples were resistant to methicillin (MRSA, and 111 (79.3% were susceptible to methicillin (MSSA. The constitutive resistance phenotype (cMLSB was observed in 20 (14.3% MRSA samples and in 5 (3.6% MSSA samples, whereas the inducible resistance phenotype (iMLSB was observed in 3 (2.1% MRSA samples and in 8 (5.8% MSSA samples. Conclusions The D-test is essential for detecting the iMLSB phenotype because the early identification of this phenotype allows clinicians to choose an appropriate treatment for patients. Furthermore, this test is simple, easy to perform and inexpensive.

  8. A method for the measurement of shielding effectiveness of planar samples requiring no sample edge preparation or contact

    OpenAIRE

    Marvin, Andrew C.; Dawson, Linda; Flintoft, Ian Dand; Dawson, John F.

    2009-01-01

    A method is presented for the measurement of shielding effectiveness of planar materials with nonconducting surfaces such as carbon fiber composites. The method overcomes edge termination problems with such materials by absorbing edge-diffracted energy. A dynamic range of up to 100 dB has been demonstrated over a frequency range of 1-8.5 GHz, depending on the size of the sample under test. Comparison with ASTM D4935 and nested reverberation measurements of shielding effectiveness shows good a...

  9. Evaluation of intestinal sampling sites in pigs at slaughter for assessing antibiotic resistance level in swine herds

    DEFF Research Database (Denmark)

    Jensen, Annette Nygaard; Thanou, Olga; Axelsdottir, Aslaug

    2012-01-01

    In the EU project SafeOrganic, the objective is to compare the level of antibiotic resistance in conventional pig herds with the level in organic pig herds, where a restricted use of antimicrobials is expected to result in less resistant bacteria. For such survey, sampling at the abattoir opposed......-colon in some pigs indicating a relatively short intestinal passage time after ingestion and then the risk of finding bacteria not originating from the host pig but from the environment. However, the proportion of the TET resistant E. coli in the large intestine appeared relatively stable over time, though...... generally a little lower than in rectal swabs. Accordingly, it seems that testing for the level of TET resistant E. coli at slaughter can allow for comparison of the presence of TET resistance also at herd level. Ongoing investigations of pigs from different herds should allow for further conclusions...

  10. Laboratory measurements of electrical resistivity versus water content on small soil cores

    Science.gov (United States)

    Robain, H.; Camerlynck, C.; Bellier, G.; Tabbagh, A.

    2003-04-01

    concentration in water may be neglected during the sewage of macro voids as it corresponds to a small quantity of water for the studied samples. Soil solid components are generally electrical insulators, the conduction of electrical current only lies on two phenomenon occurring in water : (i) volume conduction controlled by the electrolyte concentration in water and the geometrical characteristics of macro voids network ; (ii) surface conduction controlled by the double diffuse layer that depends on the solid-liquid interactions, the specific surface of clay minerals and the geometry of particles contacts. For the water contained in macro voids the preeminent phenomenon seems to be volume conduction while for the water contained in micro voids, it seems to be surface conduction. This hypothesis satisfyingly explains the shape of the electrical resistivity versus water content curves obtained for three different oxisols with clayey, clayey-sandy and sandy-clayey texture. [1] Archie G.E. 1942. The electrical resistivity log as an aid in determining some reservoirs characteristics. Trans. AIME, 146, 54-67. [2] Braudeau E. et al. 1999. New device and method for soil shrinkage curve measurement and characterization. S.S.S.A.J., 63(3), 525-535.

  11. Surface Resistance Measurements and Estimate of the Beam-Induced Resistive Wall Heating of the LHC Dipole Beam Screen

    CERN Document Server

    Caspers, Friedhelm; Ruggiero, F; Tan, J

    1999-01-01

    An estimate of the resistive losses in the LHC beam screen is given from cold surface resistance measurements using the shielded pair technique, with particular emphasis on the effect of a high magnetic field. Two different copper coating methods, namely electro-deposition and co-lamination, have been evaluated. Experimental data are compared with theories including the anomalous skin effect and the magneto-resistance effect. It is shown whether the theory underestimates or not the losses depends strongly on the RRR value, on the magnetic field and on the surface characteristics. In the pessimistic case and for nominal machine parameters, the estimated beam-induced resistive wall heating can be as large as 260 mW/m for two circulating beams.

  12. Sampling

    CERN Document Server

    Thompson, Steven K

    2012-01-01

    Praise for the Second Edition "This book has never had a competitor. It is the only book that takes a broad approach to sampling . . . any good personal statistics library should include a copy of this book." —Technometrics "Well-written . . . an excellent book on an important subject. Highly recommended." —Choice "An ideal reference for scientific researchers and other professionals who use sampling." —Zentralblatt Math Features new developments in the field combined with all aspects of obtaining, interpreting, and using sample data Sampling provides an up-to-date treat

  13. Identification of pyrimethamine- and chloroquine-resistant Plasmodium falciparum in Africa between 1984 and 1998: genotyping of archive blood samples

    Directory of Open Access Journals (Sweden)

    Saito-Nakano Yumiko

    2011-12-01

    Full Text Available Abstract Background Understanding the geographical distribution of drug resistance of Plasmodium falciparum is important for the effective treatment of malaria. Drug resistance has previously been inferred mainly from records of clinical resistance. However, clinical resistance is not always consistent with the parasite's genetic resistance. Thus, molecular identification of the parasite's drug resistance is required. In Africa, clinical resistance to pyrimethamine (Pyr and chloroquine (CQ was evident before 1980 but few studies investigating the genetic resistance to these drugs were conducted before the late 1990s. In this study, genotyping of genes involved in resistance to Pyr and CQ was performed using archive blood samples from Africa between 1984 and 1998. Methods Parasite DNA was extracted from P. falciparum-infected blood smears collected from travellers returning to Japan from Africa between 1984 and 1998. Genotypes of the dihydrofolate reductase gene (dhfr and CQ-resistance transporter gene (pfcrt were determined by polymerase chain reaction amplification and sequencing. Results Genotyping of dhfr and pfcrt was successful in 59 and 80 samples, respectively. One wild-type and seven mutant dhfr genotypes were identified. Three dhfr genotypes lacking the S108N mutation (NRSI, ICSI, IRSI; amino acids at positions 51, 59, 108, and 164 with mutations underlined were highly prevalent before 1994 but reduced after 1995, accompanied by an increase in genotypes with the S108N mutation. The dhfr IRNI genotype was first identified in Nigeria in 1991 in the present samples, and its frequency gradually increased. However, two double mutants (ICNI and NRNI, the latter of which was exclusively found in West Africa, were more frequent than the IRNI genotype. Only two pfcrt genotypes were found, the wild-type and a Southeast Asian type (CVIET; amino acids at positions 72-76 with mutations underlined. The CVIET genotype was already present as early as

  14. VanA-type vancomycin-resistant enterococci in equine and swine rectal swabs and in human clinical samples.

    Science.gov (United States)

    de Niederhäusern, Simona; Sabia, Carla; Messi, Patrizia; Guerrieri, Elisa; Manicardi, Giuliano; Bondi, Moreno

    2007-09-01

    Vancomycin-resistant enterococci (VRE) in healthy people and in food-producing animals seems to be quite common in Europe. The existence of this community reservoir of VRE has been associated with the massive use of avoparcin in animal husbandry. Eight years after the avoparcin ban in Europe, we investigated the incidence of VanA enterococci, their resistance patterns, and the mobility of their glycopeptide-resistance determinants in a sampling of animal rectal swabs and clinical specimens. A total of 259 enterococci isolated from equine, swine, and clinical samples were subcultured on KF-streptococcus agar (Difco Laboratories, Detroit, MI) supplemented with vancomycin and teicoplanin; 7 (6.7%), 10 (16%), and 8 (8.6%) respectively were found to be glycopeptides resistant (VanA phenotype). Slight differences in antimicrobial resistance patterns resulted among VRE recovered from the different sources. Polymerase chain reaction amplification demonstrated the presence of the vanA gene cluster and its extrachromosomal location in VRE plasmid DNA. VanA resistance was transferred in 7 out of 25 mating experiments, 4 with clinical, 2 with swine, and only 1 with equine donors. The conjugative plasmids of animal strains showed a high homology in the restriction profiles, unlike plasmids of clinical microrganisms. Our observations confirmed the possible horizontal transfer of VanA plasmids across different strains and, consequently, the diffusion of the vancomycin-resistance determinants.

  15. Occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs

    DEFF Research Database (Denmark)

    Pedersen, Karl; Pedersen, Kristina; Jensen, Helene

    2007-01-01

    Objectives: To study the occurrence of antimicrobial resistance among common bacterial pathogens from dogs and relate resistance patterns to data on consumption of antimicrobials. Methods: The antimicrobial susceptibility patterns of 201 Staphylococcus intermedius, 37 Streptococcus canis, 39....... intermedius and Proteus isolates. Conclusions: This investigation provided data on occurrence of antimicrobial resistance in important pathogenic bacteria from dogs, which may be useful for the small animal practitioner. Resistance was low to the compounds that were most often used, but unfortunately......, these compounds were broad-spectrum. Data on resistance and usage may form a background for the establishment of a set of recommendations for prudent use of antimicrobials for companion animals....

  16. Assessing Local Risk of Rifampicin-Resistant Tuberculosis in KwaZulu-Natal, South Africa Using Lot Quality Assurance Sampling.

    Directory of Open Access Journals (Sweden)

    Christine L Heidebrecht

    Full Text Available KwaZulu-Natal (KZN has the highest burden of notified multidrug-resistant tuberculosis (MDR TB and extensively drug-resistant (XDR TB cases in South Africa. A better understanding of spatial heterogeneity in the risk of drug-resistance may help to prioritize local responses.Between July 2012 and June 2013, we conducted a two-way Lot Quality Assurance Sampling (LQAS study to classify the burden of rifampicin (RIF-resistant TB among incident TB cases notified within the catchment areas of seven laboratories in two northern and one southern district of KZN. Decision rules for classification of areas as having either a high- or low-risk of RIF resistant TB (based on proportion of RIF resistance among all TB cases were based on consultation with local policy makers.We classified five areas as high-risk and two as low-risk. High-risk areas were identified in both Southern and Northern districts, with the greatest proportion of RIF resistance observed in the northernmost area, the Manguzi community situated on the Mozambique border.Our study revealed heterogeneity in the risk of RIF resistant disease among incident TB cases in KZN. This study demonstrates the potential for LQAS to detect geographic heterogeneity in areas where access to drug susceptibility testing is limited.

  17. Influenza virus drug resistance: a time-sampled population genetics perspective.

    Directory of Open Access Journals (Sweden)

    Matthieu Foll

    2014-02-01

    Full Text Available The challenge of distinguishing genetic drift from selection remains a central focus of population genetics. Time-sampled data may provide a powerful tool for distinguishing these processes, and we here propose approximate Bayesian, maximum likelihood, and analytical methods for the inference of demography and selection from time course data. Utilizing these novel statistical and computational tools, we evaluate whole-genome datasets of an influenza A H1N1 strain in the presence and absence of oseltamivir (an inhibitor of neuraminidase collected at thirteen time points. Results reveal a striking consistency amongst the three estimation procedures developed, showing strongly increased selection pressure in the presence of drug treatment. Importantly, these approaches re-identify the known oseltamivir resistance site, successfully validating the approaches used. Enticingly, a number of previously unknown variants have also been identified as being positively selected. Results are interpreted in the light of Fisher's Geometric Model, allowing for a quantification of the increased distance to optimum exerted by the presence of drug, and theoretical predictions regarding the distribution of beneficial fitness effects of contending mutations are empirically tested. Further, given the fit to expectations of the Geometric Model, results suggest the ability to predict certain aspects of viral evolution in response to changing host environments and novel selective pressures.

  18. Analysis of Samples Treated by Resistance Test Method Exposed to Accelerated Aging

    Directory of Open Access Journals (Sweden)

    Irena Bates

    2015-09-01

    Full Text Available Global awareness that packaging has to be fully adequate and of high quality, is gradually increasing. That is why printing inks and substrates, which have no detrimental effect on packed products, should be considered a compulsory precondition for food and tobacco packaging. Printing inks that have been developed in the recent years, especially for food and tobacco packaging, have low-odour and low migration into the printing substrate during the drying process. Their migration into the printing substrate is within the acceptable limits and has no detrimental effect in terms of food safety. Another extremely important element of prints in high quality food and tobacco packaging is their stability, as they have to be resistant to liquids and chemicals, which are a part of packed product. The selection of an appropriate printing substrate is also extremely relevant, since the interaction of substrate with printing inks should have zero effect on the packed product and should not change the physical appearance of packaging. This paper presents the results of analysing the stability of laboratory samples printed with low-migration inks, observed immediately after the printing (unaged and two treatments of accelerated aging. The accelerated aging of prints was conducted in order to simulate conditions in which food and tobacco packaging can be found due to the prolonged indoors storage. The stability of prints was analysed based on optical characteristics by observing the prints’ relative reflectance curves.

  19. Note: A non-invasive electronic measurement technique to measure the embedded four resistive elements in a Wheatstone bridge sensor

    Science.gov (United States)

    Ravelo Arias, S. I.; Ramírez Muñoz, D.; Cardoso, S.; Ferreira, R.; Freitas, P.

    2015-06-01

    The work shows a measurement technique to obtain the correct value of the four elements in a resistive Wheatstone bridge without the need to separate the physical connections existing between them. Two electronic solutions are presented, based on a source-and-measure unit and using discrete electronic components. The proposed technique brings the possibility to know the mismatching or the tolerance between the bridge resistive elements and then to pass or reject it in terms of its related common-mode rejection. Experimental results were taken in various Wheatstone resistive bridges (discrete and magnetoresistive integrated bridges) validating the proposed measurement technique specially when the bridge is micro-fabricated and there is no physical way to separate one resistive element from the others.

  20. Note: A non-invasive electronic measurement technique to measure the embedded four resistive elements in a Wheatstone bridge sensor

    Energy Technology Data Exchange (ETDEWEB)

    Ravelo Arias, S. I.; Ramírez Muñoz, D. [Department of Electronic Engineering, University of Valencia, Avda. de la Universitat, s/n, 46100-Burjassot (Spain); Cardoso, S. [INESC Microsystems and Nanotechnologies (INESC-MN) and Institute for Nanosciences and Nanotechnologies, R. Alves Redol 9, Lisbon 1000-029 (Portugal); Ferreira, R. [INL-International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga 4715-31 (Portugal); Freitas, P. [INESC Microsystems and Nanotechnologies (INESC-MN) and Institute for Nanosciences and Nanotechnologies, R. Alves Redol 9, Lisbon 1000-029 (Portugal); INL-International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga 4715-31 (Portugal)

    2015-06-15

    The work shows a measurement technique to obtain the correct value of the four elements in a resistive Wheatstone bridge without the need to separate the physical connections existing between them. Two electronic solutions are presented, based on a source-and-measure unit and using discrete electronic components. The proposed technique brings the possibility to know the mismatching or the tolerance between the bridge resistive elements and then to pass or reject it in terms of its related common-mode rejection. Experimental results were taken in various Wheatstone resistive bridges (discrete and magnetoresistive integrated bridges) validating the proposed measurement technique specially when the bridge is micro-fabricated and there is no physical way to separate one resistive element from the others.

  1. History of target and special sample preparation at the Central Bureau for Nuclear Measurements

    International Nuclear Information System (INIS)

    Audenhove, J.V.; Pauwels, J.

    1981-01-01

    The Central Bureau for Nuclear Measurements is a laboratory whose special aim was the fabrication, analysis and distribution of foils, targets and other samples for nuclear measurements. Preparation and assaying techniques that have been used since 1963 are briefly reviewed

  2. Online junction temperature measurement via internal gate resistance during turn-on

    DEFF Research Database (Denmark)

    Baker, Nick; Munk-Nielsen, Stig; Liserre, Marco

    2014-01-01

    A new method for junction temperature measurement of power semiconductor switches is presented. The measurement exploits the temperature dependent resistance of the temperature sensitive electrical parameter (TSEP): the internal gate resistance. This dependence can be observed during the normal s...... TSEP based measurement methods, primarily being: an absence of any dependence on operating conditions such as load current, and the potential to achieve higher sensitivity (20mV/C or more) than alternative TSEPs....

  3. Screening and contact precautions – A survey on infection control measures for multidrug-resistant bacteria in German university hospitals

    Directory of Open Access Journals (Sweden)

    Lena M. Biehl

    2017-04-01

    Full Text Available Abstract To assess the scope of infection control measures for multidrug-resistant bacteria in high-risk settings, a survey among university hospitals was conducted. Fourteen professionals from 8 sites participated. Reported policies varied largely with respect to the types of wards conducting screening, sample types used for screening and implementation of contact precautions. This variability among sites highlights the need for an evidence-based consensus of current infection control policies.

  4. Study of antibiotic resistance of staph aureus and coagulase negative staphylococci isolated from patient samples

    Directory of Open Access Journals (Sweden)

    M Anvary

    2006-04-01

    Full Text Available Introduction: Drug resistant Staphylococci are the most important agents of nosocomial infections. In this survey, effect of different antibiotics on these bacteria and their drug resistance was investigated. Methods: The study included 500 strains of Staphylococci. Minimum Inhibitory Concentrations of all antibiotics was determined by the broth macro dilution technique and standard methods from the National Committee for Clinical Laboratory Standards. Result : Oxacillin resistance of S. aureus was 14.2% and that of coagulase-negative staphylococci was 53.4%. The activity of different antibiotics is presented in detail. Conclusion: Surveillance of strains resistant to methicillin is necessary.

  5. Irreversibility in transformation behavior of equiatomic nickel-titanium alloy by electrical resistivity measurement

    International Nuclear Information System (INIS)

    Matsumoto, Hitoshi

    2004-01-01

    Measurements of the electrical resistivity were precisely performed on shape memory Ni 50 Ti 50 alloy in order to reveal the irreversible behavior of the thermoelastic martensitic transformation with thermal cycling. The hump in the electrical resistivity during cooling is enhanced with increasing the number of complete thermal cycles to result in a peak, although no peak in the electrical resistivity is observed on the reverse transformation during heating. The electrical resistivity in the low-temperature phase, of which the temperature dependence is linear, increases with increasing the number of complete thermal cycles. The temperature coefficient of the electrical resistivity in the temperature region of the high-temperature phase increases with elevating the temperature. The transformation is strongly influenced by incomplete thermal cycles to result in a peak in the resistivity even on the reverse transformation after incomplete thermal cycling. It is thought that the anomalous behavior such as enhancement of a resistivity-peak, the increase in the electrical resistivity of the low-temperature phase, and the nonlinear relation between the resistivity and the temperature in the high-temperature phase are attributable to the appearance of an intermediate phase stabilized by transformation-induced defects, the accumulation of the transformation-induced defects, and the electron scattering due to the softening of a phonon mode in the high-temperature phase, respectively. It proved useful to make more accurate measurements of the electrical resistivity in order to investigate the intrinsic behavior of the transformation in NiTi

  6. Quantifying the improvement of surrogate indices of hepatic insulin resistance using complex measurement techniques.

    Directory of Open Access Journals (Sweden)

    John G Hattersley

    Full Text Available We evaluated the ability of simple and complex surrogate-indices to identify individuals from an overweight/obese cohort with hepatic insulin-resistance (HEP-IR. Five indices, one previously defined and four newly generated through step-wise linear regression, were created against a single-cohort sample of 77 extensively characterised participants with the metabolic syndrome (age 55.6 ± 1.0 years, BMI 31.5 ± 0.4 kg/m(2; 30 males. HEP-IR was defined by measuring endogenous-glucose-production (EGP with [6-6(2H(2] glucose during fasting and euglycemic-hyperinsulinemic clamps and expressed as EGP*fasting plasma insulin. Complex measures were incorporated into the model, including various non-standard biomarkers and the measurement of body-fat distribution and liver-fat, to further improve the predictive capability of the index. Validation was performed against a data set of the same subjects after an isoenergetic dietary intervention (4 arms, diets varying in protein and fiber content versus control. All five indices produced comparable prediction of HEP-IR, explaining 39-56% of the variance, depending on regression variable combination. The validation of the regression equations showed little variation between the different proposed indices (r(2 = 27-32% on a matched dataset. New complex indices encompassing advanced measurement techniques offered an improved correlation (r = 0.75, P<0.001. However, when validated against the alternative dataset all indices performed comparably with the standard homeostasis model assessment for insulin resistance (HOMA-IR (r = 0.54, P<0.001. Thus, simple estimates of HEP-IR performed comparable to more complex indices and could be an efficient and cost effective approach in large epidemiological investigations.

  7. First environmental sample containing plasmid-mediated colistin-resistant ESBL-producing Escherichia coli detected in Norway.

    Science.gov (United States)

    Jørgensen, Silje Bakken; Søraas, Arne; Arnesen, Lotte Stenfors; Leegaard, Truls; Sundsfjord, Arnfinn; Jenum, Pål A

    2017-09-01

    We hereby report the detection of the plasmid borne mcr-1 gene conferring colistin resistance in an extended-spectrum β-lactamase (ESBL) producing Escherichia coli ST10 strain retrieved from seawater at a public beach in Norway. The sample was collected in September 2010 and was investigated by whole-genome sequencing in 2016. This report illustrates that E. coli strains carrying plasmid-mediated colistin resistance genes have also reached areas where this drug is hardly used at all. Surveillance of colistin resistance in environmental, veterinary, and human strains is warranted also in countries where colistin resistance is rare in clinical settings. © 2017 APMIS. Published by John Wiley & Sons Ltd.

  8. Measurements of clothing evaporative resistance using a sweating thermal manikin: an overview

    Science.gov (United States)

    WANG, Faming

    2017-01-01

    Evaporative resistance has been widely used to describe the evaporative heat transfer property of clothing. It is also a critical variable in heat stress models for predicting human physiological responses in various environmental conditions. At present, sweating thermal manikins provide a fast and cost-effective way to determine clothing evaporative resistance. Unfortunately, the measurement repeatability and reproducibility of evaporative resistance are rather low due to the complicated moisture transfer processes through clothing. This review article presents a systematical overview on major influential factors affecting the measurement precision of clothing evaporative resistance measurements. It also illustrates the state-of-the-art knowledge on the development of test protocol to measure clothing evaporative resistance by means of a sweating manikin. Some feasible and robust test procedures for measurement of clothing evaporative resistance using a sweating manikin are described. Recommendations on how to improve the measurement accuracy of clothing evaporative resistance are addressed and expected future trends on development of advanced sweating thermal manikins are finally presented. PMID:28566566

  9. Slipping on pedestrian surfaces: methods for measuring and evaluating the slip resistance.

    Science.gov (United States)

    Wetzel, Christoph; Windhövel, Ulrich; Mewes, Detlef; Ceylan, Orhan

    2015-01-01

    Tripping, slipping and falling accidents are among the types of accident with a high incidence. This article describes the requirements concerning slip resistance, as well as the state of the art of slip resistance measurement standards in the European Community and the USA. The article also describes how risk assessment can be performed in the field.

  10. Considerations for sampling nuclear materials for SNM accounting measurements. Special nuclear material accountability report

    International Nuclear Information System (INIS)

    Brouns, R.J.; Roberts, F.P.; Upson, U.L.

    1978-05-01

    This report presents principles and guidelines for sampling nuclear materials to measure chemical and isotopic content of the material. Development of sampling plans and procedures that maintain the random and systematic errors of sampling within acceptable limits for SNM(Special Nuclear Materials) accounting purposes are emphasized

  11. Experimental verification of air flow rate measurement for representative isokinetic air sampling in ventilation stacks

    International Nuclear Information System (INIS)

    Okruhlica, P.; Mrtvy, M.; Kopecky, Z.

    2008-01-01

    Nuclear facilities are obliged to monitor their discharge's influence on environment. Main monitored factions in NPP's ventilation stacks are usually noble gasses, particulates and iodine. These factions are monitored in air sampled from ventilation stack by means of sampling rosette and bypass followed with on-line measuring monitors and balance sampling devices with laboratory evaluations. Correct air flow rate measurement and representative iso-kinetic air sampling system is essential for physical correct and metrological accurate evaluation of discharge influence on environment. Pairs of measuring sensors (Anemometer, pressure gauge, thermometer and humidity meter) are symmetrically placed in horizontal projection of stack on positions based on measured air flow velocity distribution characteristic, Analogically diameter of sampling rosette nozzles and their placement in the middle of 6- 7 annuluses are calculated for assurance of representative iso-kinetic sampling. (authors)

  12. Comparison of two assays for molecular determination of rifampin resistance in clinical samples from patients with Buruli ulcer disease.

    Science.gov (United States)

    Jansson, Moritz; Beissner, Marcus; Phillips, Richard Odame; Badziklou, Kossi; Piten, Ebekalisai; Maman, Issaka; Sarfo, Fred Stephen; Huber, Kristina Lydia; Rhomberg, Agata; Symank, Dominik; Wagner, Magdalena; Wiedemann, Franz; Nitschke, Jörg; Banla Kere, Abiba; Herbinger, Karl-Heinz; Adjei, Ohene; Löscher, Thomas; Bretzel, Gisela

    2014-04-01

    This study evaluates a novel assay for detecting rifampin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of the samples tested, the assay was 100% M. ulcerans specific and yielded >98% analyzable sequences with a lower limit of detection of 100 to 200 copies of the target sequence.

  13. Comparison of Two Assays for Molecular Determination of Rifampin Resistance in Clinical Samples from Patients with Buruli Ulcer Disease

    OpenAIRE

    Jansson, Moritz; Beissner, Marcus; Phillips, Richard Odame; Badziklou, Kossi; Piten, Ebekalisai; Maman, Issaka; Sarfo, Fred Stephen; Huber, Kristina Lydia; Rhomberg, Agata; Symank, Dominik; Wagner, Magdalena; Wiedemann, Franz; Nitschke, Jörg; Banla Kere, Abiba; Herbinger, Karl-Heinz

    2014-01-01

    This study evaluates a novel assay for detecting rifampin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of the samples tested, the assay was 100% M. ulcerans specific and yielded >98% analyzable sequences with a lower limit of detection of 100 to 200 copies of the target sequence.

  14. Antimicrobial resistance in the Bacteroides fragilis group in faecal samples from patients receiving broad-spectrum antibiotics

    DEFF Research Database (Denmark)

    Møller Hansen, Kia Cirkeline; Ferløv-Schwensen, Simon Andreas; Henriksen, Daniel Pilsgaard

    2017-01-01

    the prevalence of the carbapenem resistance gene cfiA in B. fragilis in this patient group, and 3) to determine the association between previous antibiotic treatment and reduced susceptibility to clindamycin, meropenem, metronidazole, and piperacillin-tazobactam. Consecutive faecal samples (n = 197) were...

  15. Detection of clarithromycin-resistant Helicobacter pylori by polymerase chain reaction using residual samples from rapid urease test

    Directory of Open Access Journals (Sweden)

    Jae-Sik Jeon

    2017-01-01

    Full Text Available Background: Approximately 50% of the world population is infected with Helicobacter pylori, which corresponds to a high infection rate. Furthermore, the incidence of antibiotic-resistant H. pylori has increased with the recent rise in use of antibiotics for H. pylori elimination, suggesting growing treatment failures. Aim: The study was aimed to assess the use of residual samples from rapid urease test (RUT for biomolecular testing as an effective and accurate method to detect antibiotic-resistant H. pylori. Settings and Design: This study was a retrospective study performed using data obtained from medical records of previously isolated H. pylori strains. Materials and Methods: RUT was conducted for 5440 biopsy samples from individuals who underwent health examination in South Korea. Subsequently, 469 RUT residual samples were randomly selected and subjected to polymerase chain reaction (PCR to detect antibiotic-resistant H. pylori. Statistical Analysis Used: The Chi-square test was used to analyse categorical data. P < 0.05 was considered statistically significant. Results: The results showed a concordance between the results of PCR and conventional RUT in 450 of 469 samples, suggesting that the H. pylori PCR test is a time- and cost-effective detection method. Conclusions: This study demonstrated that PCR test can aid physicians to prescribe the appropriate antibiotics at the time of diagnosis, thus preventing the reduction in H. pylori eradication due to antibiotic resistance, averting progression to serious diseases and increasing the treatment success rate.

  16. Direct measurement of graphene contact resistivity to pre-deposited metal in buried contact test structure

    KAUST Repository

    Qaisi, Ramy M.

    2013-08-01

    We demonstrate a buried contact based novel test structure for direct contact resistivity measurement of graphene-metal interfaces. We also observe excellent contact resistivity 1 μO-cm2 without any additional surface modification suggesting that the intrinsic Au-graphene contact is sufficient for achieving devices with low contact resistance. The chemical mechanical polishing less test structure and data described herein highlights an ideal methodology for systematic screening and engineering of graphene-metal contact resistivity to enable low power high speed carbon electronics. © 2013 IEEE.

  17. Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia.

    Science.gov (United States)

    El-Tayeb, Mohamed A; Ibrahim, Abdelnasser S S; Al-Salamah, Ali A; Almaary, Khalid S; Elbadawi, Yahya B

    Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39.4%), followed by S. Paratyphi (21.2%), S. Typhimurium (15.2%), S. Typhi and S. Arizona (12.1%), respectively. Most isolates were resistant to 1st and 2nd generation cephalosporin; and aminoglycosides. Moreover, several S. enterica isolates exhibited resistance to the first-line antibiotics used for Salmonellosis treatment including ampicillin, trimethoprim-sulfamethoxazole and chloramphenicol. In addition, the results revealed the emergence of two S. enterica isolates showing resistance to third-generation cephalosporin. Analysis of resistance determinants in S. enterica strains (n=33) revealed that the resistance to β-lactam antibiotics, trimethoprim-sulfamethoxazole, chloramphenicol, and tetracycline, was attributed to the presence of carb-like, dfrA1, floR, tetA gene, respectively. On the other hand, fluoroquinolone resistance was related to the presence of mutations in gyrA and parC genes. These findings improve the information about foodborne Salmonella in Saudi Arabia, alarming the emergence of multi-drug resistant S. enterica strains, and provide useful data about the resistance mechanisms. Copyright © 2017. Published by Elsevier Editora Ltda.

  18. Material variability as measured by low temperature electrical resistivity.

    Science.gov (United States)

    Clark, A. F.; Tryon, P. V.

    1972-01-01

    Low temperature electrical resistivity was used to determine the material variability (1) between different manufacturers, (2) between different heats from the same manufacturer, and (3) within a given heat for Al 2024, Al-5% Mg alloys, Inconel 718, A286 stainless, and AISI 316. Generally, the coefficient of variation for solution annealed alloys ranged from 1.2 to 14% between manufacturers, 0.8 to 5.1% between heats, and 0.1 to 1.6% within a heat with stainless steels at the low ends and Al 2024 at the high ends. The variability is increased if the material is in a precipitation-hardened condition. A statistical analysis suggests that the variability within a heat is non-normal.

  19. Comparison of microstickies measurement methods. Part I, sample preparation and measurement methods

    Science.gov (United States)

    Mahendra R. Doshi; Angeles Blanco; Carlos Negro; Gilles M. Dorris; Carlos C. Castro; Axel Hamann; R. Daniel Haynes; Carl Houtman; Karen Scallon; Hans-Joachim Putz; Hans Johansson; R.A. Venditti; K. Copeland; H.-M. Chang

    2003-01-01

    Recently, we completed a project on the comparison of macrostickies measurement methods. Based on the success of the project, we decided to embark on this new project on comparison of microstickies measurement methods. When we started this project, there were some concerns and doubts principally due to the lack of an accepted definition of microstickies. However, we...

  20. Sample preparation for measuring cow's progesterone in radioimmunoassay technique application

    International Nuclear Information System (INIS)

    Nuning Duria; Budi Santoso; Nuniek Lelananingtiyas; Wiranto Budi Santoso

    2010-01-01

    Sample preparation for measuring cow's progesterone in radioimmunoassay technique application has been done. Preparation includes the preparation of samples of cow's milk samples that has been carried out artificial insemination on day 0, 11 and 21. Then the standard making of progesterone in the form of fresh milk or skim milk (non fat) that has been removed its progesterone and through a series of chemical processes. The last is the preparation work on samples using the RIA kit I 125 . With this milk samples preparation, samples can be counted using RIA counter so that concentration of progesterone hormone can be determined accurately. (author)

  1. Evaluation of Porosity and Saturation Degree by Laboratory Joint Measurements of Velocity and Resistivity: A Model Improvement

    Science.gov (United States)

    Carrara, E.; Mazzacca, A.; Pece, R.; Roberti, N.; Vanorio, T.

    This paper illustrates the laboratory procedures and experiments carried out on samples with different lithologies and reconstructed samples in order to test and implement an electroseismic model (Carrara et al., 1994) which allows the evaluation of porosity and the saturation degree of rocks. The testing was conducted by comparing porosity (Φ) and saturation degree (Sw ) values, obtained from measured resistivity (ρ) and elastic wave velocity (VP ) by means of appropriate formulations, with the values of Φ and Sw previously calculated by geotechnics and employed as standards. The experimental results have led to a good definition of the model to be proposed as an application method in the field of hydrogeology.

  2. Design of an automatic sample changer for the measurement of neutron flux by gamma spectrometry

    International Nuclear Information System (INIS)

    Gago, Javier; Bruna, Ruben; Baltuano, Oscar; Montoya, Eduardo; Descreaux, Killian

    2014-01-01

    This paper presents calculus, selection and components design for the construction of an automatic system in order to measure neutron flux in a working nuclear reactor by the gamma spectrometry technique using samples irradiated on the RP-10 nucleus. This system will perform the measurement of interchanging 100 samples in a programed and automatic way, reducing operation time by the user and obtaining more accurate measures. (authors).

  3. Precision measurement of the quantized anomalous Hall resistance at zero magnetic field

    Science.gov (United States)

    Götz, Martin; Fijalkowski, Kajetan M.; Pesel, Eckart; Hartl, Matthias; Schreyeck, Steffen; Winnerlein, Martin; Grauer, Stefan; Scherer, Hansjörg; Brunner, Karl; Gould, Charles; Ahlers, Franz J.; Molenkamp, Laurens W.

    2018-02-01

    In the quantum anomalous Hall effect, the edge states of a ferromagnetically doped topological insulator exhibit quantized Hall resistance and dissipationless transport at zero magnetic field. Up to now, however, the resistance was experimentally assessed using standard transport measurement techniques which are difficult to trace to the von-Klitzing constant RK with high precision. Here, we present a metrologically comprehensive measurement, including a full uncertainty budget, of the resistance quantization of V-doped (Bi,Sb)2Te3 devices without the external magnetic field. For the deviation of the quantized anomalous Hall resistance from RK, we determined a value of 0.17 ± 0.25 ppm, the smallest and most precise value reported to date. This is a step towards realization of a practical zero-field quantum resistance standard which in combination with the Josephson effect could provide the universal quantum units standard in the future.

  4. Simultaneous atomic force microscopy measurement of topography and contact resistance of metal films and carbon nanotubes

    International Nuclear Information System (INIS)

    Stadermann, M.; Grube, H.; Boland, J.J.; Papadakis, S.J.; Falvo, M.R.; Superfine, R.; Washburn, S.

    2003-01-01

    We present a quartz tuning-fork-based atomic force microscopy (AFM) setup that is capable of mapping the surface contact resistance while scanning topography. The tuning-fork setup allows us to use etched Pt/Ir tips, which have higher durability and better conductivity than probes used in earlier AFM conductance measurements. The performance of the method is demonstrated with contact resistance measurements of gold lines on silicon dioxide and carbon nanotubes on graphite

  5. Use of Direct Current Resistivity Measurements to Assess AISI 304 Austenitic Stainless Steel Sensitization

    OpenAIRE

    Mesquita, Ramaiany Carneiro; Mecury, José Manoel Rivas; Tanaka, Auro Atsumi; Sousa, Regina Célia de

    2015-01-01

    This paper describes the feasibility of using direct current electrical resistivity measurements to evaluate AISI 304 austenitic stainless steel sensitization. ASTM A262 – Practice A and double loop electrochemical potentiodynamic reactivation (DL-EPR) tests were performed to assess the degree of sensitization (DoS) qualitatively and quantitatively, and electrical resistivity (ER) was measured by the four-point direct-current potential drop method. The results indicate that the DoS incr...

  6. Implications for differences in LWD resistivity measurements in NanTroSEIZE boreholes within the Nankai Trough, Offshore Japan

    Science.gov (United States)

    Martin, K. M.; Gulick, S. P.; Flemings, P. B.; Tudge, J.

    2009-12-01

    IODP Expedition 314, the first drilling cruise of the Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE), acquired logging while drilling (LWD) data at five sites, including gamma ray, sonic velocity and resistivity logs. Density, photoelectric factor (PEF) and neutron porosity logs were also acquired at sites C0001 and C0002. Five different resistivity logs were acquired by LWD on this expedition, including shallow, medium and deep button resistivities, a ring resistivity and resistivity at the bit. These logs all have different depths of investigation and sampling intervals. As such, the effects of fluid invasion, washouts and variations in the formation itself vary from log to log. The differences between the various readings, particularly between those with the deepest and shallowest depths of penetration can indicate the degree of invasion or washout in the borehole. This degree of invasion may in turn shed some light on the relative permeability of the formation itself. The differences in the five resistivity readings acquired on Expedition 314 do not consistently vary directly or inversely with caliper readings, implying that differences between the deep and shallow button resistivities are not simply related to washouts, but likely also contain a signal related to formation invasion. We have investigated the variations in the differences between resistivities, with an emphasis on the occurrence of variations not associated with washouts. Site C0002 in the Kumano Basin provides a complete sedimentary section in which to examine how fluctuations in the differences between resistivity logs correspond to lithological changes. The basin sediments are interpreted to be primarily sandy-silty turbidites based on gamma ray log values and variations, as well as density, PEF and resistivity log values. A particularly clay-rich log unit (interpreted based on gamma ray and PEF log values) at the base of the basin sediments corresponds to an area of similarity

  7. Influence of Sample Temperature for Measurement Accuracy with FT-NIR Spectroscopy.

    Science.gov (United States)

    Dvořák, Lukáš; Fajman, Martin; Sustova, Kvetoslava

    2017-03-01

    This study monitored the influence of milk samples temperature on the measuring accuracy of FT-NIR spectroscopy in milk content analysis. Reference methods were used to determine dry matter, fat, protein, and lactose content in cow's milk. Milk samples were measured in reflectance mode on an integrating sphere with the use of a compression cuvette and a transflectance cuvette, ensuring a beam trajectory length of h = 0.2 mm. The samples were measured at 18, 20, 22, 24, and 40°C, and analyses were performed at 100 scans at a resolution of 8 cm-1. The measurements were influenced by the temperatures of the samples during the evaluation of all analyzed constituents (P determination of milk constituents requires maintaining, during analysis, the temperature conditions of the samples and the conditions for which the spectrophotometer calibration was designed.

  8. Mass thickness measurement of dual-sample by dual-energy X-rays

    International Nuclear Information System (INIS)

    Chen Mincong; Li Hongmei; Chen Ziyu; Shen Ji

    2008-01-01

    X-ray equivalent energy can be used to measure mass thicknesses of materials. Based on this, a method of mass thickness measurement of dual-sample was discussed. It was found that in the range of sample mass thickness under investigation, the equivalent mass attenuation coefficient of a component could be used to compute mass thicknesses of a dual-sample, with relative errors of less than 5%. Mass thickness measurement of a fish sample was performed, and the fish bone and flesh could be displayed separately and clearly by their own mass thicknesses. This indicates that the method is effective in mass thickness measurement of dual-sample of suitable thicknesses. (authors)

  9. Measurements of the anomalous RF surface resistance of niobium using a dielectric resonator

    International Nuclear Information System (INIS)

    Moffat, D.; Bolore, M.; Bonin, B.; Jacques, E.; Safa, H.

    1996-01-01

    The surface resistance of high and low residual resistance ratio (RRR) niobium plates at 4.2 K and 1.8 K has been measured as a function of many processing and testing parameters. A dielectric resonator was used instead of a resonant cavity. This resonator offered the ability to make many, sensitive measurements with an efficient use of time and helium. It was found that the surface resistance, R s , of RRR = 190 niobium increased noticeably from the theoretical value if the cooling rate was slower than ∼ 10 K/min. (author)

  10. Relationship between antibiotic resistance genes and metals in residential soil samples from Western Australia.

    Science.gov (United States)

    Knapp, Charles W; Callan, Anna C; Aitken, Beatrice; Shearn, Rylan; Koenders, Annette; Hinwood, Andrea

    2017-01-01

    Increasing drug-resistant infections have drawn research interest towards examining environmental bacteria and the discovery that many factors, including elevated metal conditions, contribute to proliferation of antibiotic resistance (AR). This study examined 90 garden soils from Western Australia to evaluate predictions of antibiotic resistance genes from total metal conditions by comparing the concentrations of 12 metals and 13 genes related to tetracycline, beta-lactam and sulphonamide resistance. Relationships existed between metals and genes, but trends varied. All metals, except Se and Co, were related to at least one AR gene in terms of absolute gene numbers, but only Al, Mn and Pb were associated with a higher percentage of soil bacteria exhibiting resistance, which is a possible indicator of population selection. Correlations improved when multiple factors were considered simultaneously in a multiple linear regression model, suggesting the possibility of additive effects occurring. Soil-metal concentrations must be considered when determining risks of AR in the environment and the proliferation of resistance.

  11. Automated low energy photon absorption equipment for measuring internal moisture and density distributions of wood samples

    International Nuclear Information System (INIS)

    Tiitta, M.; Olkkonen, H.; Lappalainen, T.; Kanko, T.

    1993-01-01

    Automated equipment for measuring the moisture and density distributions of wood samples was developed. Using a narrow beam of gamma rays, the equipment scans the wood samples, which are placed on the moving belt. The moisture measurement is based on the 241 Am photon absorption technique (59.5 keV), where the difference of the linear absorption coefficients of the moist and dry wood is measured. The method requires no knowledge of the thickness of the specimen. The density estimation method is based on the measurement of the linear attenuation coefficient of wood. Comprehensive software including image processing was developed for treatment of the numerical values of the measurements. (author)

  12. Estimating subsoil resistance to nitrate leaching from easily measurable pedological properties

    Directory of Open Access Journals (Sweden)

    Fábio Keiti Nakagawa

    2012-11-01

    Full Text Available Leaching of nitrate (NO3- can increase the groundwater concentration of this anion and reduce the agronomical effectiveness of nitrogen fertilizers. The main soil property inversely related to NO3- leaching is the anion exchange capacity (AEC, whose determination is however too time-consuming for being carried out in soil testing laboratories. For this reason, this study evaluated if more easily measurable soil properties could be used to estimate the resistance of subsoils to NO3- leaching. Samples from the subsurface layer (20-40 cm of 24 representative soils of São Paulo State were characterized for particle-size distribution and for chemical and electrochemical properties. The subsoil content of adsorbed NO3- was calculated from the difference between the NO3- contents extracted with 1 mol L-1 KCl and with water; furthermore, NO3- leaching was studied in miscible displacement experiments. The results of both adsorption and leaching experiments were consistent with the well-known role exerted by AEC on the nitrate behavior in weathered soils. Multiple regression analysis indicated that in subsoils with (i low values of remaining phosphorus (Prem, (ii low soil pH values measured in water (pH H2O, and (iii high pH values measured in 1 moL L-1 KCl (pH KCl, the amounts of surface positive charges tend to be greater. For this reason, NO3- leaching tends to be slower in these subsoils, even under saturated flow condition.

  13. Reconstructing physical activity monitoring outcome measures using an interval sampling approach.

    Science.gov (United States)

    Amor, James D; James, Christopher J

    2017-07-01

    Smart-watches and wearables are becoming more and more popular and this popularity is resulting in an increased interest in their use in research and clinical settings for monitoring physical activity. The battery life of these devices is of some concern, especially when they are put under increased duress through the need to constantly sample from their accelerometers. In this paper a novel approach to sampling from the accelerometer is explored whereby the accelerometer is sampled in intervals to avoid constant sampling. Results show that through reducing the sampling time and sampling in discontinuous intervals that outcome measures of time spent in sedentary, moderate and vigorous activity can be reconstructed to within acceptable error levels when compared to the same outcome measures calculated from continually sampled data.

  14. Evaluation of sugar yeast consumption by measuring electrical medium resistance

    Directory of Open Access Journals (Sweden)

    Martin Lucas Zamora

    2013-12-01

    Full Text Available The real-time monitoring of alcoholic fermentation (sugar consumption is very important in industrial processes. Several techniques (i.e., using a biosensor have been proposed to realize this goal. In this work, we propose a new method to follow sugar yeast consumption. This novel method is based on the changes in the medium resistance (Rm that are induced by the CO2 bubbles produced during a fermentative process. We applied a 50-mV and 700-Hz signal to 75 ml of a yeast suspension in a tripolar cell. A gold electrode was used as the working electrode, whereas an Ag/AgCl electrode and a stainless-steel electrode served as the reference and counter electrodes, respectively. We then added glucose to the yeast suspension and obtained a 700% increase in the Rm after 8 minutes. The addition of sucrose instead of glucose as the carbon source resulted in a 1200% increase in the Rm. To confirm that these changes are the result of CO2 bubbles in the fermentation medium, we designed a tetrapolar cell in which CO2 gas was insufflated at the bottom of the cell and concluded that the changes were due to CO2 bubbles produced during the fermentation. Consequently, this new method is a low-cost and rapid technology to follow the sugar consumption in yeast.

  15. Antibiotic Resistance Pattern and Identification of Vancomycin Resistance Genes in Enterococcus spp. Isolated from Environmental Samples in Southern Fars province

    Directory of Open Access Journals (Sweden)

    Hosseini Fatemeh

    2017-07-01

    Conclusion: The results indicated that the VRE strains are widespread in the studied area, therefore there is an urgent need for prudent use of vancomycin and implementation of control measures to prevent the environmental spread of VRE strains.

  16. Direct sample positioning and alignment methodology for strain measurement by diffraction

    Science.gov (United States)

    Ratel, N.; Hughes, D. J.; King, A.; Malard, B.; Chen, Z.; Busby, P.; Webster, P. J.

    2005-05-01

    An ISO (International Organization for Standardization) TTA (Technology Trends Assessment) was published in 2001 for the determination of residual stress using neutron diffraction which identifies sample alignment and positioning as a key source of strain measurement error. Although the measurement uncertainty by neutron and synchrotron x-ray diffraction for an individual measurement of lattice strain is typically of the order of 10-100×10-6, specimens commonly exhibit strain gradients of 1000×10-6mm-1 or more, making sample location a potentially considerable source of error. An integrated approach to sample alignment and positioning is described which incorporates standard base-plates and sample holders, instrument alignment procedures, accurate digitization using a coordinate measuring machine and automatic generation of instrument control scripts. The methodology that has been developed is illustrated by the measurement of the transverse residual strain field in a welded steel T-joint using neutrons.

  17. Direct sample positioning and alignment methodology for strain measurement by diffraction

    International Nuclear Information System (INIS)

    Ratel, N.; Hughes, D.J.; King, A.; Malard, B.; Chen, Z.; Busby, P.; Webster, P.J.

    2005-01-01

    An ISO (International Organization for Standardization) TTA (Technology Trends Assessment) was published in 2001 for the determination of residual stress using neutron diffraction which identifies sample alignment and positioning as a key source of strain measurement error. Although the measurement uncertainty by neutron and synchrotron x-ray diffraction for an individual measurement of lattice strain is typically of the order of 10-100x10 -6 , specimens commonly exhibit strain gradients of 1000x10 -6 mm -1 or more, making sample location a potentially considerable source of error. An integrated approach to sample alignment and positioning is described which incorporates standard base-plates and sample holders, instrument alignment procedures, accurate digitization using a coordinate measuring machine and automatic generation of instrument control scripts. The methodology that has been developed is illustrated by the measurement of the transverse residual strain field in a welded steel T-joint using neutrons

  18. Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

    Science.gov (United States)

    Fernando, Dinesh M.; Tun, Hein Min; Poole, Jenna; Patidar, Rakesh; Li, Ru; Mi, Ruidong; Amarawansha, Geethani E. A.; Fernando, W. G. Dilantha; Khafipour, Ehsan; Farenhorst, Annemieke

    2016-01-01

    ABSTRACT Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, β-lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. IMPORTANCE In this study, we highlight the poor microbiological quality of drinking water in a First Nations community in Canada. We examined the coliform load as well as the presence of antibiotic resistance genes in these samples. This study examined the presence of antibiotic-resistant genes in drinking water samples from a First Nations Community in Canada. We believe that our findings are of considerable significance, since the issue of poor water quality in First Nations communities in Canada is often ignored, and our findings will help shed some light on this important issue. PMID:27235436

  19. The Analysis Of Accuracy Of Selected Methods Of Measuring The Thermal Resistance Of IGBTs

    Directory of Open Access Journals (Sweden)

    Górecki Krzysztof

    2015-09-01

    Full Text Available In the paper selected methods of measuring the thermal resistance of an IGBT (Insulated Gate Bipolar Transistor are presented and the accuracy of these methods is analysed. The analysis of the measurement error is performed and operating conditions of the considered device, at which each measurement method assures the least measuring error, are pointed out. Theoretical considerations are illustrated with some results of measurements and calculations.

  20. The concept of measurement of thermal neutron absorption cross section in small samples

    International Nuclear Information System (INIS)

    Czubek, J.A.

    1980-01-01

    Theoretical principles of the method of measurement of the absorption cross section for thermal neutrons are presented in the one velocity approach. In consecutive measurements the sample investigated is enveloped in shells of a known moderator of varying thickness and irradiated with the pulsed beam of fast neutrons. The die-away rate of thermal neutrons escaping from such a system is measured. The absorption cross section of the unknown sample is found as the intersection of the experimental curve (die-away rate viz. thickness of the moderator) with the theoretical one calculated for the case of the zero value of the material buckling of the sample. (author)

  1. The state of the art of the impact of sampling uncertainty on measurement uncertainty

    Science.gov (United States)

    Leite, V. J.; Oliveira, E. C.

    2018-03-01

    The measurement uncertainty is a parameter that marks the reliability and can be divided into two large groups: sampling and analytical variations. Analytical uncertainty is a controlled process, performed in the laboratory. The same does not occur with the sampling uncertainty, which, because it faces several obstacles and there is no clarity on how to perform the procedures, has been neglected, although it is admittedly indispensable to the measurement process. This paper aims at describing the state of the art of sampling uncertainty and at assessing its relevance to measurement uncertainty.

  2. Airflow resistivity instrument for in situ measurement on the earth's ground surface

    Science.gov (United States)

    Zuckerwar, A. J.

    1983-01-01

    An airflow resistivity instrument features a novel specimen holder, especially designed for in situ measurement on the earth's ground surface. This capability eliminates the disadvantages of prior intrusive instruments, which necessitate the removal of a test specimen from the ground. A prototype instrument can measure airflow resistivities in the range 10-5000 cgs rayl/cm, at specimen depths up to 15.24 cm (6 in.), and at differential pressures up to 2490.8 dyn sq cm (1 in. H2O) across the specimen. Because of the close relationship between flow resistivity and acoustic impedance, this instrument should prove useful in acoustical studies of the earth's ground surface. Results of airflow resistivity measurements on an uncultivated grass field for varying values of moisture content are presented.

  3. Automation for measuring the surface resistivity of RPC by Arduino motor shield

    International Nuclear Information System (INIS)

    Pandey, A.; Kumar, A.; Singh, M.K.; Marimuthu, N.; Singh, V.

    2016-01-01

    India Based Neutrino Observatory (INO) is planning to use ∼ 30,000 Resistive Plate Chamber (RPC) detectors of size 2m x 2m. Each resistive plate chamber is made up of glass or Bakelite whose resistance is of the order 10 12 Ω/cm 2 , and two pick-up panels. Both glass electrodes of the detector have one side (outer) painted with thin and uniform coating of graphite paint. This provides the resistivity of range 100 - 300 kΩ per square. This range of resistivity provides distribution of voltage over the surface of glass electrodes. Uniform coating will provide uniform electric field inside the glass plate chamber and high resistivity value will help in confining the induced charge on the surface. It is one of the necessary criteria for good RPC detector that the coating of graphite paint should be uniform. Therefore it is required to measure the resistivity for the verification of uniformity. Last few years we have been focusing our efforts in this direction. In this connection, we made sequential improvement from the previous work. The present system is cost effective, automatically recording measurement in the computer, fast and accurate. Earlier MOTOR BEE ver. 5.0 microcontroller was used which was an imported and costly material. After massive search in the local market Arduino microcontroller was discovered, which controls the movement of all motors and it also saves the value of resistivity in the computer automatically

  4. Error Analysis of Ceramographic Sample Preparation for Coating Thickness Measurement of Coated Fuel Particles

    International Nuclear Information System (INIS)

    Liu Xiaoxue; Li Ziqiang; Zhao Hongsheng; Zhang Kaihong; Tang Chunhe

    2014-01-01

    The thicknesses of four coatings of HTR coated fuel particle are very important parameters. It is indispensable to control the thickness of four coatings of coated fuel particles for the safety of HTR. A measurement method, ceramographic sample-microanalysis method, to analyze the thickness of coatings was developed. During the process of ceramographic sample-microanalysis, there are two main errors, including ceramographic sample preparation error and thickness measurement error. With the development of microscopic techniques, thickness measurement error can be easily controlled to meet the design requirements. While, due to the coated particles are spherical particles of different diameters ranged from 850 to 1000μm, the sample preparation process will introduce an error. And this error is different from one sample to another. It’s also different from one particle to another in the same sample. In this article, the error of the ceramographic sample preparation was calculated and analyzed. Results show that the error introduced by sample preparation is minor. The minor error of sample preparation guarantees the high accuracy of the mentioned method, which indicates this method is a proper method to measure the thickness of four coatings of coated particles. (author)

  5. Standardized Method for Measuring Collection Efficiency from Wipe-sampling of Trace Explosives.

    Science.gov (United States)

    Verkouteren, Jennifer R; Lawrence, Jeffrey A; Staymates, Matthew E; Sisco, Edward

    2017-04-10

    One of the limiting steps to detecting traces of explosives at screening venues is effective collection of the sample. Wipe-sampling is the most common procedure for collecting traces of explosives, and standardized measurements of collection efficiency are needed to evaluate and optimize sampling protocols. The approach described here is designed to provide this measurement infrastructure, and controls most of the factors known to be relevant to wipe-sampling. Three critical factors (the applied force, travel distance, and travel speed) are controlled using an automated device. Test surfaces are chosen based on similarity to the screening environment, and the wipes can be made from any material considered for use in wipe-sampling. Particle samples of the explosive 1,3,5-trinitroperhydro-1,3,5-triazine (RDX) are applied in a fixed location on the surface using a dry-transfer technique. The particle samples, recently developed to simulate residues made after handling explosives, are produced by inkjet printing of RDX solutions onto polytetrafluoroethylene (PTFE) substrates. Collection efficiency is measured by extracting collected explosive from the wipe, and then related to critical sampling factors and the selection of wipe material and test surface. These measurements are meant to guide the development of sampling protocols at screening venues, where speed and throughput are primary considerations.

  6. Measurement invariance of the Multigroup Ethnic Identity Measure (MEIM) across Bulgarian, Dutch and Greek samples

    NARCIS (Netherlands)

    Mastrotheodoros, S.; Dimitrova, R.; Motti-Stefanidi, F.; Abubakar, A.; van de Schoot, R.

    2013-01-01

    The Multigroup Ethnic Identity Measure (MEIM; Phinney, 1992) is a widely used instrument to quantify the way people think, feel, and behave regarding their ethnic origin. This instrument is commonly used to compare groups of people from different ethnic and/or cultural backgrounds. However, in order

  7. Measurement invariance of the Multigroup Ethnic Identity Measure (MEIM) across Bulgarian, Dutch and Greek samples

    NARCIS (Netherlands)

    Mastrotheodoros, S.; Dimitrova, R.; Motti-Stefanidi, F.; Abubakar Ali, A.; Van de Schoot, R.

    2012-01-01

    The Multigroup Ethnic Identity Measure (MEIM; Phinney, 1992) is a widely used instrument to quantify the way people think, feel, and behave regarding their ethnic origin. This instrument is commonly used to compare groups of people from different ethnic and/or cultural backgrounds. However, in order

  8. Installation for measurement of the Hall emf and magnetoresistance in low-resistance materials by the amplitude modulation method

    International Nuclear Information System (INIS)

    Kolla, E.V.; Luk'yanova, L.N.

    1979-01-01

    An installation is described for measuring the Hall electromotive force and the magnetorsistance ΔR(B)/R of low-resistance materials by means of the amplitude modulation method with double synchronous detection. An active filter, synchronous detectors, a phase shifter, rejector filter, scale amplifier and squarelaw generator are the basic units of the installation. The installation permits to record ΔR(B)/R approximately 10 -5 and the minimum Hall electromotive force of 10 -9 . Measurement results of the resistivity temperature dependencies, of the Hall coefficient and of the magnetoresistance of a Bisub(2)Tesub(2,7)Sesub(0.3) sample in the temperature range of 77-300 K are given

  9. Multiple drug resistance of Aeromonas hydrophila isolates from Chicken samples collected from Mhow and Indore city of Madhyapradesh

    Directory of Open Access Journals (Sweden)

    Kaskhedikar

    2009-02-01

    Full Text Available Fourteen antibacterial agents belonging to 9 different groups of antibiotics viz. aminoglycosides, cephalosporins, nitrofurantoin, fluroquinolones, chloramphenicol, sulphonamides, tetracyclines, penicillin and polymixin were used for in vitro sensitivity testing of Aeromonas hydrophila isolated from fifteen samples of chicken collected from retail shops in Mhow city. The sensitivity (100% was attributed to ciprofloxacin, cefuroxime, ceftriaxone, cephotaxime, chloramphenicol, gentamycin, kanamycin, nitrofurantoin, nalidixic acid and ofloxacin followed by oxytetracycline (50%. All the isolates were resistant to ampicillin and colistin antibiotics. That means, none of the isolates were found to be sensitive for penicillin and polymixin group of antibiotics. Multiple drug resistance was also observed in all A. hydrophila isolates. Out of total isolates, 100% were resistant to two antimicrobial drugs and 50% to three drugs. [Vet. World 2009; 2(1.000: 31-32

  10. Measurement of tritium permeation through resistant materials near room temperature

    International Nuclear Information System (INIS)

    Maienschein, J.; DuVal, V.; McMurphy, F.; Uribe, F.; Musket, R.; Brown, D.

    1985-01-01

    To measure tritium permeation through low-permeability materials at 50 to 170 0 C, we use highly-sensitive liquid scintillation counting to detect the permeating tritium. To validate our method, we conducted extensive experiments with copper, for which much data exists for comparison. We report permeability of tritium through copper at 50, 100, and 170 0 C, and discuss details of the experimental technique. Further plans are outlined. 15 refs., 5 figs., 1 tab

  11. Measurements of {sup 55}Fe activity in activated steel samples with GEMPix

    Energy Technology Data Exchange (ETDEWEB)

    Curioni, A. [CERN, 1211 Geneva 23 (Switzerland); Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Dinar, N. [CERN, 1211 Geneva 23 (Switzerland); Université de Paris VII, 5 rue Thomas-Mann, 75013 Paris (France); La Torre, F.P. [CERN, 1211 Geneva 23 (Switzerland); Leidner, J., E-mail: johannes.leidner@cern.ch [CERN, 1211 Geneva 23 (Switzerland); RWTH Aachen, Templergraben 55, 52056 Aachen (Germany); Murtas, F. [CERN, 1211 Geneva 23 (Switzerland); INFN-LNF, Via E. Fermi 40, 00044 Frascati (Italy); Puddu, S.; Silari, M. [CERN, 1211 Geneva 23 (Switzerland)

    2017-03-21

    In this paper we present a novel method, based on the recently developed GEMPix detector, to measure the {sup 55}Fe content in samples of metallic material activated during operation of CERN accelerators and experimental facilities. The GEMPix, a gas detector with highly pixelated read-out, has been obtained by coupling a triple Gas Electron Multiplier (GEM) to a quad Timepix ASIC. Sample preparation, measurements performed on 45 samples and data analysis are described. The calibration factor (counts per second per unit specific activity) has been obtained via measurements of the {sup 55}Fe activity determined by radiochemical analysis of the same samples. Detection limit and sensitivity to the current Swiss exemption limit are calculated. Comparison with radiochemical analysis shows inconsistency for the sensitivity for only two samples, most likely due to underestimated uncertainties of the GEMPix analysis. An operative test phase of this technique is already planned at CERN.

  12. Assessment of the Bacteriological Quality of Food Samples May Offer Clues the Antibiotic Resistance Pattern

    Directory of Open Access Journals (Sweden)

    Enayatollah Kalantar

    2015-02-01

    Full Text Available Background: Foodborne illnesses continue to be a leading cause of morbidity and mortality worldwide; however, the burden of diseases caused by food-borne pathogens remains largely unknown. Objectives: The aim of the present study was to culture-confirmed the bacterial profile and their antibiotic resistant pattern in Food and Drug Laboratory, Alborz University of Medical Sciences, Karaj, Iran. Patients and Methods: A total of 22 bacteria including of Staphylococcus aureus, Klebsiella spp and E. coli were presumptive isolated from the traditional ice cream, cream pastries, sausage, and salami by the Official Food Microbiology Laboratory, Deputy of Food and Drug Administration, Alborz University of Medical Sciences, Karaj, Iran, and sent to the Research Center Laboratory, Alborz University of Medical Sciences, to confirm the bacterial spp by multiplex polymerase chain reaction. These isolates were also checked for their antimicrobial resistance pattern according to CLSI guideline. Results: The highest rate of contamination was with Klebsiella spp 09 (40.9%, followed by S. aureus 07 (31.8%, E. coli 06 (27.27%, as reported by the Official Food Microbiology Laboratory of Alborz University of Medical Sciences. Gel electrophoresis of the isolates shows the 600bp bp and 80 bp gene among S. aureus and E. coli respectively. The antibiotic resistant pattern in case of Klensiella spp showed that 6 (66.6% Klensiella spp were resistant to Penicillin and Cotrimoxazole. Similarly, penicillin and amoxicillin were found the highest resistant antibiotic against 83.3% E. coli, however, ceftriaxone showed the highest sensitivity against 100% E. coli isolates. Conclusions: In conclusion, Klebsiella spp, S. aureus and E. coli are contaminants of food specimens obtained from food industries in Karaj, Iran; they constitute a serious health risk for human population. Moreover, the principal purpose of this study is to increase awareness of the antibiotic resistance of

  13. Uncertainty measurement in the homogenization and sample reduction in the physical classification of rice and beans

    Directory of Open Access Journals (Sweden)

    Dieisson Pivoto

    2016-04-01

    Full Text Available ABSTRACT: The study aimed to i quantify the measurement uncertainty in the physical tests of rice and beans for a hypothetical defect, ii verify whether homogenization and sample reduction in the physical classification tests of rice and beans is effective to reduce the measurement uncertainty of the process and iii determine whether the increase in size of beans sample increases accuracy and reduces measurement uncertainty in a significant way. Hypothetical defects in rice and beans with different damage levels were simulated according to the testing methodology determined by the Normative Ruling of each product. The homogenization and sample reduction in the physical classification of rice and beans are not effective, transferring to the final test result a high measurement uncertainty. The sample size indicated by the Normative Ruling did not allow an appropriate homogenization and should be increased.

  14. Note: Development of a microfabricated sensor to measure thermal conductivity of picoliter scale liquid samples.

    Science.gov (United States)

    Park, Byoung Kyoo; Yi, Namwoo; Park, Jaesung; Kim, Dongsik

    2012-10-01

    This paper presents a thermal analysis device, which can measure thermal conductivity of picoliter scale liquid sample. We employ the three omega method with a microfabricated AC thermal sensor with nanometer width heater. The liquid sample is confined by a micro-well structure fabricated on the sensor surface. The performance of the instrument was verified by measuring the thermal conductivity of 27-picoliter samples of de-ionized (DI) water, ethanol, methanol, and DI water-ethanol mixtures with accuracies better than 3%. Furthermore, another analytical scheme allows real-time thermal conductivity measurement with 5% accuracy. To the best of our knowledge, this technique requires the smallest volume of sample to measure thermal property ever.

  15. Integrating sphere for solar transmittance measurement of planar and nonplanar samples.

    Science.gov (United States)

    Symons, J G; Christie, E A; Peck, M K

    1982-08-01

    A new apparatus that incorporates an integrating sphere is described, which enables the solar transmittance of test samples to be measured as a function of both angle of incidence and azimuth angle. This apparatus was developed to perform measurements on both planar and nonplanar samples of larger dimensions than can be accommodated in a spectrophotometer. Solar transmittance measurements from this apparatus are compared with those from a Gier & Dunkle spectrophotometer for a range of sample materials, and excellent agreement has been found. Errors in solar transmittance measurement may arise from changes in the integrating sphere entrance port reflectance due to placement and then the removal of the test sample from the port. A correction procedure is derived to take account of these errors and is applicable to all single-beam integrating spheres.

  16. The use of volunteer radon measurements for radon mapping purposes: an examination of sampling bias issues.

    Science.gov (United States)

    Burke, Orlaith; Murphy, Patrick

    2011-09-01

    National and regional radon surveys are used in many nations to produce maps detailing the spatial variation of indoor radon concentrations. National surveys which are designed to be representative use either a geographically-weighted or a population-weighted sampling scheme. Additionally, many countries collect a large number of data on indoor radon concentrations from volunteers who have chosen to have the indoor radon concentration measured in their own dwellings. This work examines the representativeness of volunteer-based samples in radon measurement and explores the effect of potential volunteer bias on radon mapping results. We also investigate the influence that media attention has on volunteer sampling of indoor radon concentrations. The result of our work indicates that volunteer measurements are biased due to over-sampling of high radon areas. Consequently such volunteer radon measurements should not be used for radon mapping purposes.

  17. The methods of measuring radioactive xenon isotopes activity for CTBT noble xenon samples

    International Nuclear Information System (INIS)

    Jia Huaimao; Wang Shilian; Li Qi; Fan Yuanqing; Zhang Xinjun; Zhao Yungang; Wang Jun

    2011-01-01

    The radioactive xenon isotopes ( 131m Xe, 133 Xe, 133m Xe, 135 Xe) are some key important detecting objects for the Comprehensive Nuclear-Test-Ban Treaty (CTBT) monitoring. Currently the most important difficulties are xenon sampling, purring and radioactive xenon isotopes measuring. It describes in detail the methods of HPGe γ spectrometer and β-γ coincidence method measuring radioactive xenon isotopes. The Lab simultaneously adopted two methods to measure the noble gas sample spiked 133 Xe, and the result difference of two methods is 8%. In the end, the method of improving radioactive xenon isotopes measuring accuracy is put forward. (authors)

  18. Stability of measures from children's interviews: the effects of time, sample length, and topic.

    Science.gov (United States)

    Heilmann, John; DeBrock, Lindsay; Riley-Tillman, T Chris

    2013-08-01

    The purpose of this study was to examine the reliability of, and sources of variability in, language measures from interviews collected from young school-age children. Two 10-min interviews were collected from 20 at-risk kindergarten children by an examiner using a standardized set of questions. Test-retest reliability coefficients were calculated for 8 language measures. Generalizability theory (G-theory) analyses were completed to document the variability introduced into the measures from the child, session, sample length, and topic. Significant and strong reliability correlation coefficients were observed for most of the language sample measures. The G-theory analyses revealed that most of the variance in the language measures was attributed to the child. Session, sample length, and topic accounted for negligible amounts of variance in most of the language measures. Measures from interviews were reliable across sessions, and the sample length and topic did not have a substantial impact on the reliability of the language measures. Implications regarding the clinical feasibility of language sample analysis for assessment and progress monitoring are discussed.

  19. Measurement of actinides in environmental samples by Photo-Electron Rejecting Alpha Liquid Scintillation

    Energy Technology Data Exchange (ETDEWEB)

    Cadieux, J.R. [Westinghouse Savannah River Co., Aiken, SC (United States); Clark, S. [Savannah River Ecology Lab., Univ. of Georgia (United States); Fjeld, R.A.; Reboul, S.; Sowder, A. [Clemson Univ., SC (United States). Dept. of Environmental Systems Engineering

    1994-05-01

    This work describes the adaptation of extractive scintillation with a Photo-Electron Rejecting Alpha Liquid Scintillation (PERALS) (ORDELA, Inc.) spectrometer to the analysis of actinides in environmental samples from the Savannah River Site (SRS). Environmental quality assurance standards and actual water samples were treated by one of two methods; either a two step direct extraction, or for more complex samples, pretreatment by an extraction chromatographic separation prior to measurement of the alpha activity by PERALS.

  20. Validation of {sup 32}P Cherenkov radiation measurement method of urine samples for life science workers

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Seokwon; Park, Minjeong; Park, Seyoung; Yoo, Jaeryong; Ha, Wiho [Korea Institute of Radiological and Medical Science, Seoul (Korea, Republic of); Jang, Hanki [Korean Association for Radiation Application, Seoul (Korea, Republic of)

    2014-05-15

    In various research fields, phosphorus has been used by using behavior characteristics as the form of radiolabelled compound in natural environment and human body. Cherenkov counting method using LSC is also well known for beta emitting radionuclides with high energy. In particular, {sup 32}P is emitting beta particles with above 263 keV which is threshold energy for Cherenkov radiation effect. Using this physical characteristic, {sup 32}P measurement by Cherenkov radiation will be a good method for radiobioassay. The advantages of Cherenkov radiation counting for {sup 32}P are to remove interferences of low energy beta and gamma emitters and reduce the expenses in counting sample preparation. Also, chemical quenching effect can be excluded because Cherenkov radiation is produced by physical phenomenon. In case of indirect measurement, minimum detectable activity (MDA) of counting samples can be improved by increasing sample volume without scintillation cocktail. Pretreatment conditions were also discussed considering the characteristics of urine samples. And real urine samples collected from workers using {sup 32}P source in the field of life sciences were measured following the practical procedure established in this study. In this study, {sup 32}P Cherenkov radiation measurement for radiation workers in the field of life science was conducted. To evaluate {sup 32}P activity of urine sample, Cherenkov radiation counting method was applied to real cases. The accuracy of measurement results were validated by using various methods. And measurement conditions for {sup 32}P counting of urine samples were also discussed to establish standardized procedures. The measurement of most samples collected from workers was below MDA. Each one case of two workers was above MDA. The 2 workers did waste management in common. The overall results did not show significant level but some cases were a little high compared with background level which is needed to evaluate dose

  1. 40 CFR 761.298 - Decisions based on PCB concentration measurements resulting from sampling.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Decisions based on PCB concentration....61(a)(6) § 761.298 Decisions based on PCB concentration measurements resulting from sampling. (a) For grid samples which are chemically analyzed individually, the PCB concentration applies to the area of...

  2. Effects of (α,n) contaminants and sample multiplication on statistical neutron correlation measurements

    International Nuclear Information System (INIS)

    Dowdy, E.J.; Hansen, G.E.; Robba, A.A.; Pratt, J.C.

    1980-01-01

    The complete formalism for the use of statistical neutron fluctuation measurements for the nondestructive assay of fissionable materials has been developed. This formalism includes the effect of detector deadtime, neutron multiplicity, random neutron pulse contributions from (α,n) contaminants in the sample, and the sample multiplication of both fission-related and background neutrons

  3. Evaluating parameterizations of aerodynamic resistance to heat transfer using field measurements

    Directory of Open Access Journals (Sweden)

    Shaomin Liu

    2007-01-01

    Full Text Available Parameterizations of aerodynamic resistance to heat and water transfer have a significant impact on the accuracy of models of land – atmosphere interactions and of estimated surface fluxes using spectro-radiometric data collected from aircrafts and satellites. We have used measurements from an eddy correlation system to derive the aerodynamic resistance to heat transfer over a bare soil surface as well as over a maize canopy. Diurnal variations of aerodynamic resistance have been analyzed. The results showed that the diurnal variation of aerodynamic resistance during daytime (07:00 h–18:00 h was significant for both the bare soil surface and the maize canopy although the range of variation was limited. Based on the measurements made by the eddy correlation system, a comprehensive evaluation of eight popularly used parameterization schemes of aerodynamic resistance was carried out. The roughness length for heat transfer is a crucial parameter in the estimation of aerodynamic resistance to heat transfer and can neither be taken as a constant nor be neglected. Comparing with the measurements, the parameterizations by Choudhury et al. (1986, Viney (1991, Yang et al. (2001 and the modified forms of Verma et al. (1976 and Mahrt and Ek (1984 by inclusion of roughness length for heat transfer gave good agreements with the measurements, while the parameterizations by Hatfield et al. (1983 and Xie (1988 showed larger errors even though the roughness length for heat transfer has been taken into account.

  4. Antibiotic resistance among Escherichia coli isolates from stool samples of children aged 3 to 14 years from Ujjain, India

    Science.gov (United States)

    2013-01-01

    Background Antibiotic resistance is a major global public health concern, particularly in settings where few treatment options are available. Limited research has been done on antibiotic resistance in Escherichia coli of Indian children at community level. Therefore we studied antibiotic resistance patterns in E. coli isolates from stool samples of children aged 3-14 years from Ujjain, Central India, to investigate associations of resistance with demographic variables. Methods Children, 3-14 years of age, were included from 30 randomly selected villages of Palwa demographic surveillance site, Ujjain, India. Parents were interviewed using a questionnaire, and stool samples were collected from participating children. E. coli were isolated from stool samples (n = 529), and susceptibility testing to 18 different antibiotics was done using standard methods. Results The proportions of isolates resistant to various antibiotics were, nalidixic acid, (45%), tetracycline (37%), ampicillin (37%), sulfamethoxazole/trimethoprim (29%) and amoxicillin/clavulanic acid (29%). No isolates were resistant to imipenem. Overall, 72% of isolates were resistant to at least one antibiotic and 33% were multi-drug resistant. High rates of cross-resistance were seen for 15 (83%) of the antibiotics studied. E. coli isolates from children with literate mothers were more resistant to penicillins and fluoroquinolones. ESBL-producers comprised 9% of the isolates. Conclusion Antibiotic resistance and cross-resistance were common in E. coli from stools of children. Resistance rates were associated with maternal literacy. PMID:24124728

  5. Evaluation of Rhipicephalus (Boophilus microplus (Acari: Ixodidae resistance to different acaricide formulations using samples from Brazilian properties

    Directory of Open Access Journals (Sweden)

    Leandro de Oliveira Souza Higa

    2016-01-01

    Full Text Available Abstract The Rhipicephalus (Boophilus microplus tick is responsible for considerable economic losses in Brazil, causing leather damage, weight loss and reduced milk production in cattle and results in the transmission of pathogens. Currently, the main method for controlling this tick is using acaricides, but their indiscriminate use is one of the major causes of resistance dissemination. In this study, the adult immersion test (AIT was used to evaluate resistance in ticks from 28 properties located in five different states (Mato Grosso do Sul, Mato Grosso, Ceará, São Paulo, e Minas Gerais and the Distrito Federal (DF of Brazil. The resistance was found in 47.64% of the repetitions demonstrating an efficacy of less than 90% in various locations throughout the country. The larvae packet test was used to evaluate samples from ten properties in four states (Mato Grosso do Sul, Mato Grosso, Minas Gerais and Espírito Santo. Spray products belonging to the main classes of acaricides, including combination formulations, were used in both types of test. New cases of resistance were found on properties within the states of Ceará, Espírito Santo and Mato Grosso, where such resistance was not previously reported.

  6. Estimation of the sampling interval error for LED measurement with a goniophotometer

    Science.gov (United States)

    Zhao, Weiqiang; Liu, Hui; Liu, Jian

    2013-06-01

    Using a goniophotometer to implant a total luminous flux measurement, an error comes from the sampling interval, especially in the situation for LED measurement. In this work, we use computer calculations to estimate the effect of sampling interval on the measuring the total luminous flux for four typical kinds of LEDs, whose spatial distributions of luminous intensity is similar to those LEDs shown in CIE 127 paper. Four basic kinds of mathematical functions are selected to simulate the distribution curves. Axial symmetric type LED and non-axial symmetric type LED are both take amount of. We consider polar angle sampling interval of 0.5°, 1°, 2°, and 5° respectively in one rotation for axial symmetric type, and consider azimuth angle sampling interval of 18°, 15°, 12°, 10° and 5° respectively for non-axial symmetric type. We noted that the error is strongly related to spatial distribution. However, for common LED light sources the calculation results show that a usage of polar angle sampling interval of 2° and azimuth angle sampling interval of 15° is recommended. The systematic error of sampling interval for a goniophotometer can be controlled at the level of 0.3%. For high precise level, the usage of polar angle sampling interval of 1° and azimuth angle sampling interval of 10° should be used.

  7. Climatologies from satellite measurements: the impact of orbital sampling on the standard error of the mean

    Directory of Open Access Journals (Sweden)

    M. Toohey

    2013-04-01

    Full Text Available Climatologies of atmospheric observations are often produced by binning measurements according to latitude and calculating zonal means. The uncertainty in these climatological means is characterised by the standard error of the mean (SEM. However, the usual estimator of the SEM, i.e., the sample standard deviation divided by the square root of the sample size, holds only for uncorrelated randomly sampled measurements. Measurements of the atmospheric state along a satellite orbit cannot always be considered as independent because (a the time-space interval between two nearest observations is often smaller than the typical scale of variations in the atmospheric state, and (b the regular time-space sampling pattern of a satellite instrument strongly deviates from random sampling. We have developed a numerical experiment where global chemical fields from a chemistry climate model are sampled according to real sampling patterns of satellite-borne instruments. As case studies, the model fields are sampled using sampling patterns of the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS and Atmospheric Chemistry Experiment Fourier-Transform Spectrometer (ACE-FTS satellite instruments. Through an iterative subsampling technique, and by incorporating information on the random errors of the MIPAS and ACE-FTS measurements, we produce empirical estimates of the standard error of monthly mean zonal mean model O3 in 5° latitude bins. We find that generally the classic SEM estimator is a conservative estimate of the SEM, i.e., the empirical SEM is often less than or approximately equal to the classic estimate. Exceptions occur only when natural variability is larger than the random measurement error, and specifically in instances where the zonal sampling distribution shows non-uniformity with a similar zonal structure as variations in the sampled field, leading to maximum sensitivity to arbitrary phase shifts between the sample distribution and

  8. Measurement of the tritium concentration in the fractionated distillate from environmental water samples

    International Nuclear Information System (INIS)

    Atkinson, Robert; Eddy, Teresa; Kuhne, Wendy; Jannik, Tim; Brandl, Alexander

    2014-01-01

    Standard procedures for the measurement of tritium in water samples often require distillation of an appropriate sample aliquot. This distillation process may result in a fractionation of tritiated water and regular light water due to the vapor pressure isotope effect, introducing either a bias or an additional contribution to the total tritium measurement uncertainty. The current study investigates the relative change in vapor pressure isotope effect in the course of the distillation process, distinguishing it from and extending previously published measurements. The separation factor as a quantitative measure of the vapor pressure isotope effect is found to assume values of 1.04 ± 0.036, 1.05 ± 0.026, and 1.07 ± 0.038, depending on the vigor of the boiling process during distillation of the sample. A lower heat setting in the experimental setup, and therefore a less vigorous boiling process, results in a larger value for the separation factor. For a tritium measurement in water samples where the first 5 mL are discarded, the tritium concentration could be underestimated by 4–7%. - Highlights: • Tritium measurements in environmental water samples. • Distilled samples. • Vapor pressure isotope effect. • Depending on boiling mode. • Potential underestimate of tritium activity concentration of 4–7%

  9. Experimental technique to measure thoron generation rate of building material samples using RAD7 detector

    International Nuclear Information System (INIS)

    Csige, I.; Szabó, Zs.; Szabó, Cs.

    2013-01-01

    Thoron ( 220 Rn) is the second most abundant radon isotope in our living environment. In some dwellings it is present in significant amount which calls for its identification and remediation. Indoor thoron originates mainly from building materials. In this work we have developed and tested an experimental technique to measure thoron generation rate in building material samples using RAD7 radon-thoron detector. The mathematical model of the measurement technique provides the thoron concentration response of RAD7 as a function of the sample thickness. For experimental validation of the technique an adobe building material sample was selected for measuring the thoron concentration at nineteen different sample thicknesses. Fitting the parameters of the model to the measurement results, both the generation rate and the diffusion length of thoron was estimated. We have also determined the optimal sample thickness for estimating the thoron generation rate from a single measurement. -- Highlights: • RAD7 is used for the determination of thoron generation rate (emanation). • The described model takes into account the thoron decay and attenuation. • The model describes well the experimental results. • A single point measurement method is offered at a determined sample thickness

  10. Prevalence of antibiotic resistant Staphylococcus aureus from raw milk samples collected from the local vendors in the region of Tirupathi, India

    Directory of Open Access Journals (Sweden)

    Sudhanthirakodi Sudhanthiramani

    2015-04-01

    Full Text Available Aim: The study was carried out with the aim to identify the suitability of the milk for consumer use with special reference to Staphylococcus aureus from milk samples collected from various local vendors and determine the antibiotic susceptibility pattern of those positive isolates. Materials and Methods: A total of 110 milk samples were collected from the local milk vendors in and around Tirupathi region of India. All the samples were enriched with buffered peptone water in 9:1 ratio and the then inoculated on baird parker agar medium with added 2% egg yolk tellurite emulsion as selective medium for S.aureus and confirmed with mannitol salt agar, Gram’s staining and biochemical tests. The typical cultural characters with coagulase-positive samples were taken as positive samples the positive samples were tested for antibiotic susceptibility with 10 different antibiotics by employing disc diffusion method. Results: Prevalence of coagulase-positive S. aureus was 39.09% (43/110 from the milk samples. The antibiotic susceptibility test of positive isolates showed high resistant toward penicillin G 37/43 (86.04% and ampicillin 32/43 (74.42%, and also showed resistant to methicillin 6/43 (13.95%, cephalothin 6/43 (13.95%, tetracycline 6/43 (13.95%, ciprofloxacin 4/43 (9.30%, enrofloxacin 3/43 (6.97%, cefoxitin 2/43 (4.65%, gentamicin 2/43 (4.65%, and co-trimoxazole 2/43 (4.65%. Many individual isolates showed resistant against two or more antibiotics in our study. Conclusion: The above study results show that the milk samples collected from local vendor having S. aureus, which can induce disease condition as well as antibiotic resistant to the humans particularly young children and old age peoples by means of consumption of raw milk and its products. This is the public health issue, which needs to be solved by educating the local vendors regarding health problems related to unhygienic milk supply and make the awareness among the consumers about this

  11. Limited Sampling Strategies for Therapeutic Drug Monitoring of Linezolid in Patients With Multidrug-Resistant Tuberculosis

    NARCIS (Netherlands)

    Alffenaar, Jan-Willem C.; Kosterink, Jos G. W.; van Altena, Richard; van der Werf, Tjip S.; Uges, Donald R. A.; Proost, Johannes H.

    Introduction: Linezolid is a potential drug for the treatment of multidrug-resistant tuberculosis but its use is limited because of severe adverse effects such as anemia, thrombocytopenia, and peripheral neuropathy. This study aimed to develop a model for the prediction of linezolid area. under the

  12. High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples

    DEFF Research Database (Denmark)

    Gosalbes, M. J.; Vallès, Y.; Jiménez-Hernández, N.

    2016-01-01

    The gastrointestinal tract (GIT) microbiota has been identified as an important reservoir of antibiotic resistance genes (ARGs) that can be horizontally transferred to pathogenic species. Maternal GIT microbes can be transmitted to the offspring, and recent work indicates that such transfer start...

  13. Antimicrobial resistance in Enterococcus spp. isolated from environmental samples in the area of intensive poultry production

    Science.gov (United States)

    In this study, we investigated antimicrobial resistance of Enterococcus spp. from different environmental compartments including litter from two farms, 12 surface and 28 groundwater sites in an area of intensive poultry production and litter application. The enumerated isolates (n=250) were tested ...

  14. Axial-scanning low-coherence interferometer method for noncontact thickness measurement of biological samples

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Do-Hyun; Song, Chul-Gyu; Ilev, Ilko K.; Kang, Jin U.

    2011-02-20

    We investigated a high-precision optical method for measuring the thickness of biological samples regardless of their transparency. The method is based on the precise measurement of optical path length difference of the end surfaces of objects, using a dual-arm axial-scanning low-coherence interferometer. This removes any consideration of the shape, thickness, or transparency of testing objects when performing the measurement. Scanning the reference simplifies the measurement setup, resulting in unambiguous measurement. Using a 1310 nm wavelength superluminescent diode, with a 65 nm bandwidth, the measurement accuracy was as high as 11.6 {mu}m. We tested the method by measuring the thickness of both transparent samples and nontransparent soft biological tissues.

  15. Improvement of the sample preparation method for AMS measurement of 36Cl in natural environment

    Science.gov (United States)

    Jiang, Songsheng; Lin, Yuanxian; Zhang, Huiying

    2004-08-01

    The isobaric interference, 36S, is a main problem in AMS measurement of 36Cl. The SO42- ion exists in the natural environment with an amount compared with the chlorine concentration. The chemical procedure should have a powerful capacity to remove sulfur from samples. Traditionally, precipitation of BaSO4 is used to separate S from the sample material. The sulfur reduction process should be repeated more than two times for measurement of the sample with a36Cl/Cl ratio down to 10-13. The multi-reduction processes take a long time and chlorine recovery is low. In the present work, a new cation-exchange-column method is used. The Ba column is used for separation of S. When the sample solution is passed through the Ba Column, SO42- are absorbed. After removal of SO42- the sample solution is passed through the H column to absorb metal ions and transfer Cl- to HCl. Then the HCl solution is passed through the Na column to convert HCl to NaCl. Finally, excess AgNO3 is added to the NaCl solution and AgCl is precipitated for AMS measurement. The chemical process only takes ∼6 h, the time much shorter than the traditional method, and the chlorine recovery is higher than ∼90%. The rainwater, groundwater and seawater samples were prepared with this method. The 36Cl/Cl ratios in those samples were measured in range of 10-13- 10-14. The method can also be used for soil, sediment and rock samples after extraction of chlorine (36Cl) from the sample materials. Some sediment and rock samples were prepared and 36Cl was measured.

  16. Sampling strategies to measure the prevalence of common recurrent infections in longitudinal studies

    Directory of Open Access Journals (Sweden)

    Luby Stephen P

    2010-08-01

    Full Text Available Abstract Background Measuring recurrent infections such as diarrhoea or respiratory infections in epidemiological studies is a methodological challenge. Problems in measuring the incidence of recurrent infections include the episode definition, recall error, and the logistics of close follow up. Longitudinal prevalence (LP, the proportion-of-time-ill estimated by repeated prevalence measurements, is an alternative measure to incidence of recurrent infections. In contrast to incidence which usually requires continuous sampling, LP can be measured at intervals. This study explored how many more participants are needed for infrequent sampling to achieve the same study power as frequent sampling. Methods We developed a set of four empirical simulation models representing low and high risk settings with short or long episode durations. The model was used to evaluate different sampling strategies with different assumptions on recall period and recall error. Results The model identified three major factors that influence sampling strategies: (1 the clustering of episodes in individuals; (2 the duration of episodes; (3 the positive correlation between an individual's disease incidence and episode duration. Intermittent sampling (e.g. 12 times per year often requires only a slightly larger sample size compared to continuous sampling, especially in cluster-randomized trials. The collection of period prevalence data can lead to highly biased effect estimates if the exposure variable is associated with episode duration. To maximize study power, recall periods of 3 to 7 days may be preferable over shorter periods, even if this leads to inaccuracy in the prevalence estimates. Conclusion Choosing the optimal approach to measure recurrent infections in epidemiological studies depends on the setting, the study objectives, study design and budget constraints. Sampling at intervals can contribute to making epidemiological studies and trials more efficient, valid

  17. Evaluation of resistance as a measure of successful tumor ablation during irreversible electroporation of the pancreas.

    Science.gov (United States)

    Dunki-Jacobs, Erik M; Philips, Prejesh; Martin, Robert C G

    2014-02-01

    Intraoperative evaluation of successful pancreatic tumor ablation using irreversible electroporation (IRE) is difficult secondary to lack of visual confirmation. The IRE generator provides feedback by reporting current (amperage), which can be used to calculate changes in tumor tissue resistance. The purpose of the study was to determine if resistance can be used to predict successful tumor ablation during IRE for pancreatic cancers. All patients undergoing pancreatic IRE from March 2010 to December 2012 were evaluated using a prospective database. Intraoperative information, including change in tumor resistance during ablation and slope of the resistance curve, were used to evaluate effectiveness of tumor ablation in terms of local failure or recurrence (LFR) and disease-free survival (DFS). A total of 65 patients underwent IRE for locally advanced pancreatic cancer. Median follow-up was 23 months. Local failure or recurrence was seen in 17 patients at 3, 6, or 9 months post IRE. Change in tumor tissue resistance and the slope of the resistance curve were both significant in predicting LFR (p = 0.02 and p = 0.01, respectively). The median local disease-free survival was 5.5 months in patients who had recurrence compared with 12.6 months in patients who did not recur (p = 0.03). Neither mean change in tumor tissue resistance nor the slope of the resistance curve significantly predicted overall DFS. Mean change in tumor tissue resistance and the slope of the resistance curve could be used intraoperatively to assess successful tumor ablation during IRE. Larger sample size and longer follow-up are needed to determine if these parameters can be used to predict DFS. Copyright © 2014 American College of Surgeons. Published by Elsevier Inc. All rights reserved.

  18. 2D Resistivity and Induced Polarization Measurement for Manganese Ore Exploration

    Science.gov (United States)

    Srigutomo, Wahyu; Trimadona; Pratomo, Prihandhanu M.

    2016-08-01

    2D Resistivity and Induced Polarization (IP) survey was conducted to delineate the presence of minerals containing manganese in form of manganese ore. The resistivity method concerns with resistivity (ohm.m) of rocks which indicates the electrical properties in terms of ability to resist the flow of electrical current. The presence of manganese in rocks generally lowers the resistivity. The Induced Polarization (IP) method deals with chargeability (in msec) which indicates the strength of polarization effects experienced by ions in the vicinity of metallic grains in rock. The presence of manganese in rocks increases the chargeability of the rock when measured using IP method. The low resistivity zones (resistivity zones may have been influenced by the presence of clay or weathered soil. In this case, the high chargeability zones will help in confirming the prospective zones caused by manganese ore. The thicknesses of the manganese ore layer vary from about 5 to 20 m based on the cross-sections. Based on the results, we estimated the geometry of the associated manganese prospective zones for resistivity (10 msec).

  19. Analysis on the phase transition behavior of Cu base bulk metallic glass by electrical resistivity measurement

    International Nuclear Information System (INIS)

    Ji, Young Su; Chung, Sung Jae; Ok, Myoung-Ryul; Hong, Kyung Tae; Suh, Jin-Yoo; Byeon, Jai Won; Yoon, Jin-Kook; Lee, Kyung Hwan; Lee, Kyung Sub

    2007-01-01

    The crystallization behavior of Cu 43 Zr 43 Al 7 Ag 7 (numbers indicate at.%) bulk metallic glass was investigated using the isothermal electrical resistivity measurements at 450 deg. C in the supercooled liquid region. The crystallization process is a single step phase transformation. To analyze the electrical resistivity reduction, microstructure evolutions were analyzed using differential scanning calorimetry, X-ray diffraction, transmission electron microscopy and small-angle X-ray scattering. The Avrami parameter of the electrical resistivity reduction step was 1.73, indicating that the crystallization process is a diffusion-controlled growth of intermetallic compounds with decreasing nucleation rate

  20. Cross-bidge Kelvin resistor (CBKR) structures for measurement of low contact resistances

    NARCIS (Netherlands)

    Stavitski, N.; Klootwijk, J.H.; van Zeijl, H.W.; Boksteen, B.K.; Boksteen, B.K.; Kovalgin, Alexeij Y.; Wolters, Robertus A.M.

    2007-01-01

    A convenient test structure for measurement of the specific contact resistance (�?c) of metal-semiconductor junctions is the CBKR structure. During last few decades the parasitic factors which may strongly affect the measurements accuracy for �?c < 10-6 Ω • cm2 have been sufficiently discussed and

  1. The Wheatstone Gadget : a simple ciruit for the measuring differential resistance variations

    NARCIS (Netherlands)

    de Bree, H.E.; Leussink, P.J.; Korthorst, M.T.; Backlund, Ylva; Jansen, Henricus V.

    1995-01-01

    This paper presents a simple circuit for measuring differential resistance variations. The Wheatstone Gadget, or The Gadget, is able to measure the same parameters as the Wheatstone Bridge in combination with an emitter-coupled pair [1], but with fewer components and fewer supply voltages. The

  2. Measurement of the specific airway resistance by plethysmography in young children accompanied by an adult

    DEFF Research Database (Denmark)

    Klug, B; Bisgaard, H

    1997-01-01

    The purpose of this study was to evaluate a procedure for measurement of specific airway resistance (sRaw) by whole body plethysmography in young awake children accompanied by an adult. sRaw was measured by a single-step procedure, omitting the measurement of the thoracic gas volume. The frequency.......8) yrs), for whom measurements made only in the presence of an accompanying adult, the CV was 8.5%. No measurements could be obtained in 22 children (17%) (mean age 2.8 (0.5) yrs). Measurements exhibited a significant frequency dependency, and electronic BTPS compensation substantially overestimated SRaw....... In conclusion, the use of electronic compensation for simulating body temperature, atmospheric pressure and saturation with water vapour introduces a bias that affects the accuracy of the estimate of specific airway resistance. Nevertheless, plethysmographic measurements with and without an accompanying adult...

  3. Decoupling pipeline influences in soil resistivity measurements with finite element techniques

    Science.gov (United States)

    Deo, R. N.; Azoor, R. M.; Zhang, C.; Kodikara, J. K.

    2018-03-01

    Periodic inspection of pipeline conditions is an important asset management strategy conducted by water and sewer utilities for efficient and economical operations of their assets in field. The Level 1 pipeline condition assessment involving resistivity profiling along the pipeline right-of-way is a common technique for delineating pipe sections that might be installed in highly corrosive soil environment. However, the technique can suffer from significant perturbations arising from the buried pipe itself, resulting in errors in native soil characterisation. To address this problem, a finite element model was developed to investigate the degree to which pipes of different a) diameters, b) burial depths, and c) surface conditions (bare or coated) can influence in-situ soil resistivity measurements using Wenner methods. It was found that the greatest errors can arise when conducting measurements over a bare pipe with the array aligned parallel to the pipe. Depending upon the pipe surface conditions, in-situ resistivity measurements can either be underestimated or overestimated from true soil resistivities. Following results based on simulations and decoupling equations, a guiding framework for removing pipe influences in soil resistivity measurements were developed that can be easily used to perform corrections on measurements. The equations require simple a-prior information on the pipe diameter, burial depth, surface condition, and the array length and orientation used. Findings from this study have immediate application and is envisaged to be useful for critical civil infrastructure monitoring and assessment.

  4. OMNY PIN—A versatile sample holder for tomographic measurements at room and cryogenic temperatures

    Science.gov (United States)

    Holler, M.; Raabe, J.; Wepf, R.; Shahmoradian, S. H.; Diaz, A.; Sarafimov, B.; Lachat, T.; Walther, H.; Vitins, M.

    2017-11-01

    Nowadays ptychographic tomography in the hard x-ray regime, i.e., at energies above about 2 keV, is a well-established measurement technique. At the Paul Scherrer Institut, currently two instruments are available: one is measuring at room temperature and atmospheric pressure, and the other, the so-called OMNY (tOMography Nano crYo) instrument, is operating at ultra-high vacuum and offering cryogenic sample temperatures down to 10 K. In this manuscript, we present the sample mounts that were developed for these instruments. Aside from excellent mechanical stability and thermal conductivity, they also offer highly reproducible mounting. Various types were developed for different kinds of samples and are presented in detail, including examples of how specimens can be mounted on these holders. We also show the first hard x-ray ptychographic tomography measurements of high-pressure frozen biological samples, in the present case Chlamydomonas cells, the related sample pins and preparation steps. For completeness, we present accessories such as transportation containers for both room temperature and cryogenic samples and a gripper mechanism for automatic sample changing. The sample mounts are not limited to x-ray tomography or hard x-ray energies, and we believe that they can be very useful for other instrumentation projects.

  5. Measurements of 222Rn and 226Ra Levels in environmental samples by using liquid scintillation counter

    International Nuclear Information System (INIS)

    Moustafa, A.S.

    2004-01-01

    The advantageous of liquid scintillation counting technique for 6 Ra determination compared with other methods are the high counting efficiency and the easier sample preparation, with no need for sample pre-concentration. In this work, liquid scintillation counting system was used to measure 222 Rn and 226 Ra levels in environmental samples. The liquid scintillation cocktail was prepared in the laboratory and was found efficient for measuring 222 Rn. Soil, sediment and TENORM samples were dried, grind, sieved and added to hydrochloric acid, in a standard scintillation vial, preloaded with the liquid scintillation cocktail. By measuring 222 Rn levels in the prepared vials, at different intervals of time after preparation, 222 Rn and 226 Ra levels were determined

  6. Development of an automatic sampling device for the continuous measurement of atmospheric carbonyls compounds

    International Nuclear Information System (INIS)

    Perraud, V.

    2007-12-01

    Two sampling strategies were studied to develop an automatic instrument for the continuous measurement of atmospheric carbonyl compounds. Because of its specificity towards carbonyls compounds, sampling by using a transfer of gaseous phase in a liquid phase associated with a simultaneous chemical derivatization of the trapped compounds was first studied. However, this method do not allow a quantitative sampling of all studied carbonyl compounds, nor a continuous measurement in the field. To overcome the difficulties, a second strategy was investigated: the cryogenic adsorption onto solid adsorbent followed by thermodesorption and a direct analysis by GC/MS. Collection efficiency using different solid adsorbents was found greater than 95% for carbonyl compounds consisting of 1 to 7 carbons. This work is a successful first step towards the realization of the automatic sampling device for a continuous measurement of atmospheric carbonyls compounds. (author)

  7. Laboratory measurement of dielectric constant and loss tangent of Indian rock samples

    Directory of Open Access Journals (Sweden)

    MAHANDRA P. SINGH

    1980-06-01

    Full Text Available The data on dielectric properties of Indian rock samples are very
    sparsely reported in the literature. Keeping this in view, we have carried
    out laboratory measurements of the dielectric constant and loss tangents
    of some samples of sandstone, quartzite, limestone, marble, dolerite and
    basalt. LCR bridge and Q-meter have been employed for measurements
    in the frequency range 10 - 10' Hz. The results have been presented in the
    form of variation of these parameters with frequency which show characteristic
    features. Observations on the effect of moisture content of these
    samples on the dielectric properties have also been reported. Further,
    correlation of petrographic studies of rock samples with measured variations
    of dielectric properties has been attempted.

  8. Progress in AMS measurement of U isotope ratios in nanogram U samples

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Kejun, E-mail: dkj401@aliyun.com [China Institute of Atomic Energy, P.O. Box 275(50), Beijing 102413 (China); He, Ming; Wang, Chen; Zhao, Xinhong; Li, Lili; Zhao, Yonggang [China Institute of Atomic Energy, P.O. Box 275(50), Beijing 102413 (China); Wang, Xianggao [College of Physics Science and Technology, Guangxi University, Nanning 530004 (China); Shen, Hongtao [College of Physics and Technology, Guangxi Normal University, Guilin 541004 (China); Wang, Xiaoming; Pang, Fangfang; Xu, Yongning; Zhao, Qingzhang; Dou, Liang; Yang, Xuran; Wu, Shaoyong; Lin, Deyu; Li, Kangning; You, Qubo; Bao, Yiwen; Hu, Yueming [China Institute of Atomic Energy, P.O. Box 275(50), Beijing 102413 (China); and others

    2015-10-15

    The determination of uranium isotopic composition in ultra-trace U samples is very important in different fields, especially for the nuclear forensics. A new Accelerator Mass Spectrometry (AMS) technique has been developed for the measurement of uranium isotopic ratios in ng level uranium samples at China Institute of Atomic Energy (CIAE). Recently, the method was further optimized and developed by using a series of blank and standard samples. The results show that the {sup 236}U at the femtogram level can be determined in nanogram U samples by the newly developed AMS technique at CIAE. The experimental setup, performances and results will be detailed in this contribution.

  9. Analysis of capillary interferometry for measuring refractive indices of minute samples.

    Science.gov (United States)

    Qi, Shengwen; Yang, Xiuqin; Zhang, Chunping; Zhang, Lianshun; Wang, Xinyu; Xu, Tang; Tian, Jianguo; Zhang, Guangyin

    2004-01-20

    A method of measuring the refractive indices of minute samples by analyzing capillary interferometry is introduced. With the interference theory of light, the intensity distribution of an interference fringe pattern formed by a cylindrical tube of a capillary is obtained, and the influence of some parameters on the fringes are discussed. The measurement accuracy and its relative problems are analyzed.

  10. Quality control of measurements made on fixed-area sample plots

    Science.gov (United States)

    Ola Lindgren

    2000-01-01

    The paper describes results from a large program for quality control of forest measurements. The performance of 87 surveyors was evaluated. Tree heights were usually measured well, whereas the counting of tree-rings on increment cores was a source of considerable bias for many surveyors. During tree count on sample plots, many surveyors had a tendency to forget trees,...

  11. Measurement of nasal nitric oxide : evaluation of six different sampling methods

    NARCIS (Netherlands)

    de Winter-de Groot, K. M.; van der Ent, C. K.

    Specific guidelines are developed for the measurement of bronchial FE(NO), however, nasal nitric oxide (nNO) measurement is not standardised yet, resulting in divergent nNO values. This study compares six different sampling methods for nNO as described in the literature, to analyse their outcome and

  12. Micro/Nano-scale Strain Distribution Measurement from Sampling Moiré Fringes.

    Science.gov (United States)

    Wang, Qinghua; Ri, Shien; Tsuda, Hiroshi

    2017-05-23

    This work describes the measurement procedure and principles of a sampling moiré technique for full-field micro/nano-scale deformation measurements. The developed technique can be performed in two ways: using the reconstructed multiplication moiré method or the spatial phase-shifting sampling moiré method. When the specimen grid pitch is around 2 pixels, 2-pixel sampling moiré fringes are generated to reconstruct a multiplication moiré pattern for a deformation measurement. Both the displacement and strain sensitivities are twice as high as in the traditional scanning moiré method in the same wide field of view. When the specimen grid pitch is around or greater than 3 pixels, multi-pixel sampling moiré fringes are generated, and a spatial phase-shifting technique is combined for a full-field deformation measurement. The strain measurement accuracy is significantly improved, and automatic batch measurement is easily achievable. Both methods can measure the two-dimensional (2D) strain distributions from a single-shot grid image without rotating the specimen or scanning lines, as in traditional moiré techniques. As examples, the 2D displacement and strain distributions, including the shear strains of two carbon fiber-reinforced plastic specimens, were measured in three-point bending tests. The proposed technique is expected to play an important role in the non-destructive quantitative evaluations of mechanical properties, crack occurrences, and residual stresses of a variety of materials.

  13. 169 Résumé Abstract Measure of uranium in some samples of ...

    African Journals Online (AJOL)

    youness

    puits et par activation neutronique. Mots-clés : Uranium, Radioactivité naturelle, Coraux, Coquilles de mollusques, lichens, travertins, Carbonates. Abstract. Measure of uranium in some samples of natural deposits in Morocco: environmental implications. In this work, we report the measurement results of uranium content in.

  14. Advantage of low-background laboratory in low-level activity samples measurements

    International Nuclear Information System (INIS)

    Vukanac, I.; Djurasevic, M.; Kandic, A.; Udovicic, V.; Banjanac, R.

    2009-01-01

    Detailed analyse of advantages of low-background laboratory in low-level activity samples measurements, over standard gamma spectrometry laboratory is presented in this paper. Comparative measurements were performed with two semiconductor HPGe spectrometers. Detection systems were with very similar characteristics. (author) [sr

  15. Using High-Resolution Hand-Held Radiometers To Measure In-Situ Thermal Resistance

    Science.gov (United States)

    Burch, Douglas M.; Krintz, Donald F.

    1984-03-01

    A field study was carried out to investigate the accuracy of using high-resolution radiometers to determine the in situ thermal resistance of building components having conventional residential construction. Two different types of radiometers were used to determine the thermal resistances of the walls of six test buildings located at the National Bureau of Standards. These radiometer thermal resistance measurements were compared to reference thermal resistance values determined from steady-state series resistance predictions, time-averaged heat-flow-sensor measurements, and guarded-hot-box measurements. When measurements were carried out 5 hours after sunset when the outdoor temperature was relatively steady and the heating plant was operated in a typical cyclic fashion, the following results were obtained: for lightweight wood-frame cavity walls, the radiometer procedures were found to distinguish wall thermal resistance 4.4 h.ft2- °F/Btu (0.77 m2•K/W) systematically higher than corresponding reference values. Such a discrimination will per-mit insulated and uninsulated walls to be distinguished. However, in the case of walls having large heat capacity (e.g., masonry and log), thermal storage effects produced large time lags between the outdoor diurnal temperature variation and the heat-flow response at the inside surface. This phenomenon caused radiometer thermal resistances to deviate substantially from corresponding reference values. This study recommends that the ANSI/ASHRAE Standard 101-1981 be modified requiring the heating plant to be operated in a typical cyclic fashion instead of being turned off prior to and during radiometer measurements.

  16. Practice and experience in traceability of radioactivity measurements of environmental samples

    International Nuclear Information System (INIS)

    Huang Zhijian

    1990-01-01

    This paper discusses some aspects on radioactivity measurement traceability and summarizes the work on quality assurance of radioactivity measurements of environmental samples in the laboratory, including transfer of standards, preparation of reference materials, and calibration of efficiency for volumse surces with Ge(Li) spectrometer. Some practical activitis regarding intercomparison of radioactivity measurements and other traceabillity-related activities are also described. Some sugestions relating to performing quality assurance are made

  17. Integron types, gene cassettes and antimicrobial resistance profile of Acinetobacter baumannii isolated from BAL samples in Babol, north of Iran.

    Science.gov (United States)

    Akrami, Fariba; Shahandashti, Elaheh Ferdosi; Yahyapour, Yousef; Sadeghi, Mohsen; Khafri, Soraya; Pournajaf, Abazar; Rajabnia, Ramazan

    2017-08-01

    Multi-drug resistant isolates of Acinetobacter baumannii have created therapeutic problems worldwide. This current study was intended to determine the Integron types, gene cassettes and antimicrobial resistance profile of A. baumannii isolated from BAL samples in Babol, north of Iran. During a 15-month period, 35 A. baumannii isolates were studied. Different classes of antimicrobial agents were used to determine the resistance ratios. Multiplex-PCR was used to detect different types of integrons and associated gene cassettes. The resistance rates to GM, FEP, AK, TOB, CP, PIP, SAM, IPM, SXT, CTX, CAZ, CL, TIM, MEM, and TZP were 85.7%, 100%, 91.4%, 68.5%, 94.3%, 88.5%, 97.1%, 94.3%, 100%, 100%, 100%, 0.0%, 91.4%, 94.3% and 91.4%, respectively. The distribution analysis of int genes showed that 25.7%, 88.6% and 28.6% of isolates carried the intI, intII and intIII genes, respectively. The prevalence of aadB, dfrA1, bla-OXA 30 and aadA1 genes were 94.3%, 77.1%, 40% and 5.7%, respectively. The current study showed that a high level of A. baumannii isolates harbor integrons in our therapeutic center, which may lead to distribution of multiple antimicrobial resistance. The different types of gene cassette arrays in the present study highlight the important role of geographical features in MDR isolates dissemination which could be credited to different profiles of drug consumption in different areas. The findings emphasized that the need for continuous surveillance to prevent distribution of multidrug resistance among A. baumannii strains in Iran. Copyright © 2017. Published by Elsevier Ltd.

  18. Measurement Invariance of the WHODAS 2.0 in a Population-Based Sample of Youth.

    Science.gov (United States)

    Kimber, Melissa; Rehm, Jürgen; Ferro, Mark A

    2015-01-01

    The World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0) is a brief measure of global disability originally developed for adults, which has since been implemented among samples of children and youth. However, evidence of its validity for use among youth, particularly measurement invariance, is lacking. Investigations of measurement invariance assess the extent to which the psychometric properties of observed items in a measure are generalizable across samples. Satisfying the assumption of measurement invariance is critical for any inferences about between-group differences. The objective of this paper was to empirically assess the measurement invariance of the 12-item interview version of the WHODAS 2.0 measure in an epidemiological sample of youth (15 to 17 years) and adults (≥ 18 years) in Canada. Multiple-group confirmatory factor analysis using a categorical variable framework allowed for the sequential testing of increasingly restrictive models to evaluate measurement invariance of the WHODAS 2.0 between adults and youth. Findings provided evidence for full measurement invariance of the WHODAS 2.0 in youth aged 15 to 17 years. The final model fit the data well: χ(2)(159) = 769.04, p WHODAS 2.0. Findings indicate that the WHODAS 2.0 is valid for making substantive comparisons of disability among youth as young as 15 years of age.

  19. Effects of Heterogeneities, Sampling Frequencies, Tools and Methods on Uncertainties in Subsurface Contaminant Concentration Measurements

    Science.gov (United States)

    Ezzedine, S. M.; McNab, W. W.

    2007-12-01

    Long-term monitoring (LTM) is particularly important for contaminants which are mitigated by natural processes of dilution, dispersion, and degradation. At many sites, LTM can require decades of expensive sampling at tens or even hundreds of existing monitoring wells, resulting in hundreds of thousands, or millions of dollars per year for sampling and data management. Therefore, contaminant sampling tools, methods and frequencies are chosen to minimize waste and data management costs while ensuring a reliable and informative time-history of contaminant measurement for regulatory compliance. The interplay play between cause (i.e. subsurface heterogeneities, sampling techniques, measurement frequencies) and effect (unreliable data and measurements gap) has been overlooked in many field applications which can lead to inconsistencies in time- histories of contaminant samples. In this study we address the relationship between cause and effect for different hydrogeological sampling settings: porous and fractured media. A numerical model has been developed using AMR-FEM to solve the physicochemical processes that take place in the aquifer and the monitoring well. In the latter, the flow is governed by the Navier-Stokes equations while in the former the flow is governed by the diffusivity equation; both are fully coupled to mimic stressed conditions and to assess the effect of dynamic sampling tool on the formation surrounding the monitoring well. First of all, different sampling tools (i.e., Easy Pump, Snapper Grab Sampler) were simulated in a monitoring well screened in different homogeneous layered aquifers to assess their effect on the sampling measurements. Secondly, in order to make the computer runs more CPU efficient the flow in the monitoring well was replaced by its counterpart flow in porous media with infinite permeability and the new model was used to simulate the effect of heterogeneities, sampling depth, sampling tool and sampling frequencies on the

  20. A Comparison of Proliferation Resistance Measures of Misuse Scenarios Using a Markov Approach

    Energy Technology Data Exchange (ETDEWEB)

    Yue,M.; Cheng, L.-Y.; Bari, R.

    2008-05-11

    Misuse of declared nuclear facilities is one of the important proliferation threats. The robustness of a facility against these threats is characterized by a number of proliferation resistance (PR) measures. This paper evaluates and compares PR measures for several misuse scenarios using a Markov model approach to implement the pathway analysis methodology being developed by the PR&PP (Proliferation Resistance and Physical Protection) Expert Group. Different misue strategies can be adopted by a proliferator and each strategy is expected to have different impacts on the proliferator's success. Selected as the probabilistic measure to represent proliferation resistance, the probabilities of the proliferator's success of misusing a hypothetical ESFR (Example Sodium Fast Reactor) facility system are calculated using the Markov model based on the pathways constructed for individual misuse scenarios. Insights from a comparison of strategies that are likely to be adopted by the proliferator are discussed in this paper.

  1. Slip resistance of winter footwear on snow and ice measured using maximum achievable incline.

    Science.gov (United States)

    Hsu, Jennifer; Shaw, Robert; Novak, Alison; Li, Yue; Ormerod, Marcus; Newton, Rita; Dutta, Tilak; Fernie, Geoff

    2016-05-01

    Protective footwear is necessary for preventing injurious slips and falls in winter conditions. Valid methods for assessing footwear slip resistance on winter surfaces are needed in order to evaluate footwear and outsole designs. The purpose of this study was to utilise a method of testing winter footwear that was ecologically valid in terms of involving actual human testers walking on realistic winter surfaces to produce objective measures of slip resistance. During the experiment, eight participants tested six styles of footwear on wet ice, on dry ice, and on dry ice after walking over soft snow. Slip resistance was measured by determining the maximum incline angles participants were able to walk up and down in each footwear-surface combination. The results indicated that testing on a variety of surfaces is necessary for establishing winter footwear performance and that standard mechanical bench tests for footwear slip resistance do not adequately reflect actual performance. Practitioner Summary: Existing standardised methods for measuring footwear slip resistance lack validation on winter surfaces. By determining the maximum inclines participants could walk up and down slopes of wet ice, dry ice, and ice with snow, in a range of footwear, an ecologically valid test for measuring winter footwear performance was established.

  2. Evaluation of metal–nanowire electrical contacts by measuring contact end resistance

    International Nuclear Information System (INIS)

    Park, Hongsik; Beresford, Roderic; Xu, Jimmy; Ha, Ryong; Choi, Heon-Jin; Shin, Hyunjung

    2012-01-01

    It is known, but often unappreciated, that the performance of nanowire (NW)-based electrical devices can be significantly affected by electrical contacts between electrodes and NWs, sometimes to the extent that it is really the contacts that determine the performance. To correctly understand and design NW device operation, it is thus important to carefully measure the contact resistance and evaluate the contact parameters, specific contact resistance and transfer length. A four-terminal pattern or a transmission line model (TLM) pattern has been widely used to measure contact resistance of NW devices and the TLM has been typically used to extract contact parameters of NW devices. However, the conventional method assumes that the electrical properties of semiconducting NW regions covered by a metal are not changed after electrode formation. In this study, we report that the conventional methods for contact evaluation can give rise to considerable errors because of an altered property of the NW under the electrodes. We demonstrate that more correct contact resistance can be measured from the TLM pattern rather than the four-terminal pattern and correct contact parameters including the effects of changed NW properties under electrodes can be evaluated by using the contact end resistance measurement method. (paper)

  3. Accurate measurement of bromine contents in plastic samples by instrumental neutron activation analysis.

    Science.gov (United States)

    Kim, I J; Lee, K S; Hwang, E; Min, H S; Yim, Y H

    2013-03-26

    Accurate measurements of bromine contents in plastic samples were made by the direct comparator instrumental neutron activation analysis (INAA). Individual factors affecting the measurements were comprehensively evaluated and compensated, including the volatility loss of bromine from standard comparators, the background bromine level in the filter papers used for preparation of the standard comparators, nuclear interference, γ-ray spectral interference and the variance among replicates of the samples. Uncertainty contributions from those factors were thoroughly evaluated and included in the uncertainty budgeting of the INAA measurement. (81)Br was chosen as the target isotope, and the INAA measurements for bromine were experimentally confirmed to exhibit good linearity within a bromine content range of 10-170 μg. The established method has been applied to the analysis of eight plastic samples: four commercially available certified reference materials (CRMs) of polyethylene and polystyrene and four acrylonitrile butadiene styrene (ABS) samples prepared as the candidate reference materials (KRISS CRM 113-01-012, -013, -014 and -015). The bromine contents of the samples were calculated at three different γ-ray energies and compared, showing good agreement. The results of the four CRMs also showed good consistency with their certified values within the stated uncertainties. Finally, the bromine contents of the ABS samples were determined with expanded uncertainties (at a 95% level of confidence) between 2.5% and 5% in a bromine content range of 25-900 mg kg(-1). Copyright © 2013 Elsevier B.V. All rights reserved.

  4. A Study of the Electrotransport in Thin Films: An Advanced Method for the Study of Electrotransport in Thin Films Using Electrical Resistance Measurements

    Science.gov (United States)

    Hummel, R. E.; Slippy, W. A., Jr.

    1970-01-01

    To date, very little is understood about electrotransport in thin films. One reason for this is the lack of methods in which electromigration can be adequately monitored dynamically. In this thesis, a new nondestructive method for the study of the electrotransport phenomenon in thin aluminum films is presented. This method makes use of electrical resistance measurements of various regions along the test sample to monitor changes in resistance resulting from electromigration. The advantage of this method is that resistance changes can be observed long before void formation can be seen using standard microscopy methods. In order to achieve the best efficiency using this method, special consideration must be given to the design of the sample. Design problems and their respective solutions are given. The results of the electrical resistance measurements are interpreted by means of photographs taken with an scanning electron microscope. The increase in resistance of the aluminum samples is interpreted to be due to void formation. The expected decrease in resistance near the anode was never observed. It is shown that mass accumulation takes on the form of hillocks and whiskers on the surface of the aluminum film, which does not contribute appreciably to conductivity.

  5. Summarizing performance for genome scale measurement of miRNA: reference samples and metrics.

    Science.gov (United States)

    Pine, P Scott; Lund, Steven P; Parsons, Jerod R; Vang, Lindsay K; Mahabal, Ashish A; Cinquini, Luca; Kelly, Sean C; Kincaid, Heather; Crichton, Daniel J; Spira, Avrum; Liu, Gang; Gower, Adam C; Pass, Harvey I; Goparaju, Chandra; Dubinett, Steven M; Krysan, Kostyantyn; Stass, Sanford A; Kukuruga, Debra; Van Keuren-Jensen, Kendall; Courtright-Lim, Amanda; Thompson, Karol L; Rosenzweig, Barry A; Sorbara, Lynn; Srivastava, Sudhir; Salit, Marc L

    2018-03-06

    The potential utility of microRNA as biomarkers for early detection of cancer and other diseases is being investigated with genome-scale profiling of differentially expressed microRNA. Processes for measurement assurance are critical components of genome-scale measurements. Here, we evaluated the utility of a set of total RNA samples, designed with between-sample differences in the relative abundance of miRNAs, as process controls. Three pure total human RNA samples (brain, liver, and placenta) and two different mixtures of these components were evaluated as measurement assurance control samples on multiple measurement systems at multiple sites and over multiple rounds. In silico modeling of mixtures provided benchmark values for comparison with physical mixtures. Biomarker development laboratories using next-generation sequencing (NGS) or genome-scale hybridization assays participated in the study and returned data from the samples using their routine workflows. Multiplexed and single assay reverse-transcription PCR (RT-PCR) was used to confirm in silico predicted sample differences. Data visualizations and summary metrics for genome-scale miRNA profiling assessment were developed using this dataset, and a range of performance was observed. These metrics have been incorporated into an online data analysis pipeline and provide a convenient dashboard view of results from experiments following the described design. The website also serves as a repository for the accumulation of performance values providing new participants in the project an opportunity to learn what may be achievable with similar measurement processes. The set of reference samples used in this study provides benchmark values suitable for assessing genome-scale miRNA profiling processes. Incorporation of these metrics into an online resource allows laboratories to periodically evaluate their performance and assess any changes introduced into their measurement process.

  6. Thermal diffusivity measurement of focused-ion-beam fabricated sample using photothermal reflectance technique.

    Science.gov (United States)

    Hua, Zilong; Ban, Heng

    2017-05-01

    Focused-Ion-Beam (FIB) can lift-off micrometer-sized samples from bulk materials for structural characterization and property measurement. The ability to determine thermophysical properties of such samples offers unique insight into the local microstructure-property relationship. A photothermal reflectance technique is developed to measure the thermal diffusivity of FIB-fabricated, micrometer-sized samples in this study. An analytic model is established to guide the experimental design and data analysis for the limited sample size and thickness. The thermal diffusivity of the sample can be extracted from a series of spatial-scan measurements at several modulated heating frequencies. To demonstrate the viability of the technique, a FIB-fabricated SiC plate with the size of 42 μm × 31 μm × 8 μm was used to represent high conductivity materials, which pose more challenges for the technique. The result compares favorably with literature values of SiC. The measurement uncertainty is quantified and possible experimental error sources are discussed. This technique is specially promising for thermal property measurements on nuclear fuels and materials.

  7. Connection of control circuits of machine for automatic measurement of radioactive samples

    International Nuclear Information System (INIS)

    Vorlicek, J.

    1984-01-01

    A windowless through-flow gas detector is used for measurement. The automatic machine is controlled by four flip-flops defining the following states: the dish replacement in the measuring space, washing, measurement, measured value print-out, and resetting. The first and second outputs of the first, second and third flip-flops are connected to six inputs of a block whose four outputs provide counter reset and stop-watch reset, washing, measurement, and print-out. Such machine control eliminates measurement errors by disabling sample measurement until air is removed from the measurement space, introduced on an unwashed dish or on several dishes passed under the detector. The elimination of this error is also guaranteed in manual operation. (M.D.)

  8. The upgraded external-beam PIXE/PIGE set-up at LABEC for very fast measurements on aerosol samples

    Energy Technology Data Exchange (ETDEWEB)

    Lucarelli, F., E-mail: lucarelli@fi.infn.it; Calzolai, G.; Chiari, M.; Giannoni, M.; Mochi, D.; Nava, S.; Carraresi, L.

    2014-01-01

    At the 3 MV Tandetron accelerator of the LABEC laboratory of INFN in Florence, an external beam facility is fully dedicated to measurements of elemental composition of atmospheric aerosol. The experimental set-up hitherto used for this kind of applications has been upgraded with the replacement of a traditional Si(Li) detector for the detection of medium–high Z elements with a silicon drift detector (SDD) with a big active area (80 mm{sup 2}) and 450 μm thickness, with the aim of obtaining better minimum detection limits (MDL) and reduce measuring times. The Upilex extraction window has been replaced by a more resistant one (Si{sub 3}N{sub 4}). A comparison between the old Si(Li) and the new SDD for aerosol samples collected on different substrata like Teflon, Kapton and Nuclepore evidenced the better performances of the SDD. It allows obtaining better results (higher counting statistics, lower MDLs) even in shorter measuring times, thus allowing very fast analysis of both daily and hourly samples.

  9. Measurements of NaI(Tl) electron response: comparison of different samples

    Energy Technology Data Exchange (ETDEWEB)

    Hull, Giulia; Choong, Woon-Seng; Moses, William W.; Bizarri, Gregory; Valentine, John D.; Payne, Stephen A.; Cherepy, Nerine J.; Reutter, Bryan W.

    2008-12-10

    This paper measures the sample to sample variation in the light yield proportionality of NaI(Tl), and so explores whether this is an invariant characteristic of the material or whether it depends on the chemical and physical properties of the tested samples. We report on the electron response of nine crystals of NaI(Tl), differing in shape, volume, age, manufacturer and quality. The proportionality has been measured at the SLYNCI facility in the energy range between 3.5 to 460 keV. We observe that while samples produced by the same manufacturer at approximately the same time have virtually identical electron response curves, there are significant sample to sample variations among crystals produced by different manufacturers or at different times. In an effort to correlate changes in the electron response with details of the scintillation mechanism, we characterized other scintillation properties, including the gamma response and the x-ray excited emission spectra and decay times, for the nine crystals. While sample to sample differences in these crystals were observed, we have been unable to identify the underlying fundamental mechanisms that are responsible for these differences.

  10. Effect of Density and Resistivity Measurement for Foam Flooding Propagation in Static Condition

    Science.gov (United States)

    Irawan, S.; Permatasari, K. A.; Bayuaji, R.

    2017-11-01

    Foam flooding is one of the method that is currently used to optimize the amount of oil in sub surface. Foam flooding is used to overcome the weakness of water injection, gas injection, and water alternating gas injection. Foam is the mixture of gas, water and surfactant. Foam propagation plays an important part in porous medium, foam propagation is related to intrinsic properties of the fluids such as density and viscosity. Resistivity of the fluids should be taken also because in the end of the experiment, method that is used to track foam propagation in porous medium is resistivity measurement. The objectives of this experiment are to obtain fluid characterization and to understand the phenomena that is possibly occurred during foam injection in porous medium, specifically, to obtain the correlation between density and resistivity measurement. Foam is generated with several concentration of solutions which are various concentration of brine, various concentration of MFOMAX, oil, and distilled water. Several tests should be taken to observe the intrinsic properties of the fluids such as density test, viscosity test, resistivity and conductivity test. Based on the experiment, correlation between density of brine and brine concentration towards resistivity is inversely proportional. Correlation between density of fluid mix with MFOMAX and MFOMAX concentration towards resistivity is proportional.

  11. Comparison of accelerator mass spectrometric measurement with liquid scintillation counting measurement for the determination of 14C in environmental samples

    International Nuclear Information System (INIS)

    Yasuike, Kaeko; Yamada, Yoshimune; Amano, Hikaru

    2010-01-01

    The concentrations of organically-bound 14 C in tree-ring cellulose of a Japanese Black Pine grown in Shika-machi (37.0 deg. N, 136.8 deg. E) and those of a Japanese Cedar grown in Kanazawa (36.5 deg. N, 136.7 deg. E), Japan, were analyzed for the ring-years from 1989 to 1998 by the accelerator mass spectrometric measurement. The results were compared with those of the same samples analyzed by the liquid scintillation counting measurement to determine the reliability of liquid scintillation counting measurement. An important result of this study is that the sensitivity and reproducibility of accelerator mass spectrometric measurement was almost equal to that of liquid scintillation counting measurement.

  12. Does progressive resistance strength training as additional training have any measured effect on functional outcomes in older hospitalized patients?

    DEFF Research Database (Denmark)

    Tibaek, Sigrid; Andersen, Christina W.; Pedersen, Sigrid F

    2014-01-01

    . PARTICIPANTS: A sample of 71 patients were successively included and randomized either to the treatment group (TG) (n = 36) or the control group (CG) (n = 35). Fifteen participants dropped out (TG n = 7; CG n = 8), leaving 56 participants with a mean age of 79 (SD 7). INTERVENTION: Participants...... = 0.05). Analysis by the mixed-effects model showed that the treatment group improved more than the control group in all outcome variables. CONCLUSION: The results indicate that for older hospitalized patients progressive resistance strength training as additional training may have an effect compared......OBJECTIVE: To evaluate the effect of progressive resistance strength training as additional training measured on functional outcomes in older hospitalized patients. DESIGN: A single-blinded randomized controlled trial. SETTING: Department of Geriatric Rehabilitation in university hospital...

  13. Polarization and resistivity measurements of post-crystallization changes in amorphous Fe-B-Si alloys

    International Nuclear Information System (INIS)

    Chattoraj, I.; Bhattamishra, A.K.; Mitra, A.

    1993-01-01

    The effects of grain growth and compositional changes on the electrochemical behavior and the resistivity of amorphous iron-boron-silicon (Fe 77.5 B 15 Si 7.5 ) alloys after crystallization were studied. Deterioration of the protective passive film was observed, along with increased annealing. Potentiodynamic polarization provided excellent information about microstructural and chemical changes. It was concluded that electrochemical measurements could be used in conjunction with resistivity measurements in direct studies of grain growth and chemical changes occurring in different phases of the devitrified alloy

  14. Inductive method to measure very small joint resistances of superconducting wires

    CERN Document Server

    Herzog, R

    1998-01-01

    In the Large Hadron Collider (LHC), which is currently being developed at CERN, about 50,000 connections of superconducting wire will be necessary to implement all circuits for powering 6000 corrector magnets with a maximum current of 600 A. To minimise the generation of ohmic heat in the cryogenic system, it is necessary to reduce the contact resistance of these connections as much as possible. This paper describes a method to measure contact resistances of splices of superconducting wires, which has been used to optimise such connections. Additionally we present results of a number of measurements on different types of connections. (2 refs).

  15. [Evaluation of the corrosion resistance of orthodontic wires by electrochemical measures and scanning electron microscopy (SEM)].

    Science.gov (United States)

    Zoghbi, André El; Klein, Lorena; Frateur, Isabelle

    2013-12-01

    The objective of this paper is to study the corrosion resistance of orthodontic wires made of different alloys (stainless steel, chrome-cobalt, nickel-titanium and β-titanium) and for the same alloy from different vendors (GAC(®), RMO(®), 3M(®) and ORMCO(®)). Different electrochemical techniques (corrosion potential monitoring as a function of immersion time, current-potential curves, electrochemical impedance spectroscopy (EIS)) were used. The wires' resistance to corrosion was measured and compared with the surface condition, assessed by scanning electron microscopy (SEM). Using the recorded data, a rating system based on the corrosion resistance of orthodontic wires was developed. The comparison of these data with the results of SEM shows that the surface chemical composition plays a primary role in the electrochemical behavior of the orthodontic wires and, unlike surface defects, is a key parameter for the corrosion resistance of the alloy. © EDP Sciences, SFODF, 2013.

  16. Drag resistance measurements for newly applied antifouling coatings and welding seams on ship hull surface

    DEFF Research Database (Denmark)

    Wang, Xueting; Olsen, S. M.; Andres, E.

    in their newly applied conditions. The effects of water absorption of newly applied antifouling coatings on frictional resistance were measured. A flexible rotor with artificial welding seams on its periphery has been designed and constructed to estimate the influence of welding seams on drag resistance. Both......Drag resistances of newly applied antifouling coatings and welding seams on ship hull surface have been investigated using a pilot-scale rotary setup. Both conventional biocide-based antifouling (AF) coatings and silicone-based fouling release (FR) coatings have been studied and compared...... the density of welding seams (number per 5 m ship side) and the height of welding seams had a significant effect on drag resistance....

  17. Interpretation of hole-to-surface resistivity measurements at Yucca Mountain, Nevada Test Site

    International Nuclear Information System (INIS)

    Daniels, J.J.; Scott, J.H.

    1981-01-01

    Hole-to-surface resistivity measurements at Yucca Mountain indicate the presence of many near-surface geologic inhomogeneities, with no definite indication of deep structural features. A resistive anomaly near drill hole UE25a-6 is interpreted as a thin, vertical, resistive body that nearly intersects the surface, and may be caused by a silicified, or calcified, fracture zone. A resistive anomaly near hole UE25a-7 is probably caused by a near surface, horizontal, lens-shaped body that may represent a devitrified zone in the Tiva Canyon Member. Many conductive anomalies were detected to the southwest of hole UE25a-4. However, these anomalies are interpreted to be caused by variations in the thickness of the surface alluvium

  18. Improvements to sample processing and measurement to enable more widespread environmental application of tritium.

    Science.gov (United States)

    Moran, James; Alexander, Thomas; Aalseth, Craig; Back, Henning; Mace, Emily; Overman, Cory; Seifert, Allen; Freeburg, Wilcox

    2017-08-01

    Previous measurements have demonstrated the wealth of information that tritium (T) can provide on environmentally relevant processes. We present modifications to sample preparation approaches that enable T measurement by proportional counting on small sample sizes equivalent to 120mg of water and demonstrate the accuracy of these methods on a suite of standardized water samples. We identify a current quantification limit of 92.2 TU which, combined with our small sample sizes, correlates to as little as 0.00133Bq of total T activity. This enhanced method should provide the analytical flexibility needed to address persistent knowledge gaps in our understanding of both natural and artificial T behavior in the environment. Copyright © 2017. Published by Elsevier Ltd.

  19. Technical developments for accurate determination of amount of samples used for TOF measurements

    Directory of Open Access Journals (Sweden)

    Terada Kazushi

    2017-01-01

    Full Text Available Activity determination of 241,243Am samples has been performed with two separate methods of calorimetry and gamma-ray spectroscopy. Decay heat measurements of the samples were carried out by using a calorimeter, and activities of the samples were accurately determined with uncertainties less than 0.45%. The primary source of uncertainty in the calorimetric method is the accuracy of available half-life data. Gamma-ray detection efficiencies of a HPGe detector were determined with uncertainties of 1.5% by combining measured efficiencies and Monte Carlo simulation. Activities of the samples were determined with uncertainties less than 2.0% by gamma-ray spectroscopy and were concordant with those of the calorimetry.

  20. Measurement of radon concentration in some water samples belonging to some adjoining areas of Pathankot, Punjab

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Ajay, E-mail: ajay782@rediffmail.com; Sharma, Sumit, E-mail: sumitshrm210@gmail.com [Post Graduate Department of Physics, DAV College, Amritsar-143001 (India)

    2015-08-28

    The study of radon concentration was measured in some areas of Pathankot district, Punjab, India, from the health hazard point of view due to radon. The exposure to radon through drinking water is largely by inhalation and ingestion. RAD 7, an electronic solid state silicon detector (Durridgeco., USA) was used to measure the radon concentration in drinking water samples of the study area. The recorded values of radon concentration in these water samples are below the recommended limit by UNSCEAR and European commission. The recommended limit of radon concentration in water samples is 4 to 40 Bq/l given by UNSCEAR [1] and European commission has recommended the safe limit for radon concentration in water sample is 100 Bq/l [2].

  1. Distinguishing HIV-1 drug resistance, accessory, and viral fitness mutations using conditional selection pressure analysis of treated versus untreated patient samples

    Directory of Open Access Journals (Sweden)

    Lee Christopher

    2006-05-01

    Full Text Available Abstract Background HIV can evolve drug resistance rapidly in response to new drug treatments, often through a combination of multiple mutations 123. It would be useful to develop automated analyses of HIV sequence polymorphism that are able to predict drug resistance mutations, and to distinguish different types of functional roles among such mutations, for example, those that directly cause drug resistance, versus those that play an accessory role. Detecting functional interactions between mutations is essential for this classification. We have adapted a well-known measure of evolutionary selection pressure (Ka/Ks and developed a conditional Ka/Ks approach to detect important interactions. Results We have applied this analysis to four independent HIV protease sequencing datasets: 50,000 clinical samples sequenced by Specialty Laboratories, Inc.; 1800 samples from patients treated with protease inhibitors; 2600 samples from untreated patients; 400 samples from untreated African patients. We have identified 428 mutation interactions in Specialty dataset with statistical significance and we were able to distinguish primary vs. accessory mutations for many well-studied examples. Amino acid interactions identified by conditional Ka/Ks matched 80 of 92 pair wise interactions found by a completely independent study of HIV protease (p-value for this match is significant: 10-70. Furthermore, Ka/Ks selection pressure results were highly reproducible among these independent datasets, both qualitatively and quantitatively, suggesting that they are detecting real drug-resistance and viral fitness mutations in the wild HIV-1 population. Conclusion Conditional Ka/Ks analysis can detect mutation interactions and distinguish primary vs. accessory mutations in HIV-1. Ka/Ks analysis of treated vs. untreated patient data can distinguish drug-resistance vs. viral fitness mutations. Verification of these results would require longitudinal studies. The result

  2. Assessing the Validity of Single-item Life Satisfaction Measures: Results from Three Large Samples

    Science.gov (United States)

    Cheung, Felix; Lucas, Richard E.

    2014-01-01

    Purpose The present paper assessed the validity of single-item life satisfaction measures by comparing single-item measures to the Satisfaction with Life Scale (SWLS) - a more psychometrically established measure. Methods Two large samples from Washington (N=13,064) and Oregon (N=2,277) recruited by the Behavioral Risk Factor Surveillance System (BRFSS) and a representative German sample (N=1,312) recruited by the Germany Socio-Economic Panel (GSOEP) were included in the present analyses. Single-item life satisfaction measures and the SWLS were correlated with theoretically relevant variables, such as demographics, subjective health, domain satisfaction, and affect. The correlations between the two life satisfaction measures and these variables were examined to assess the construct validity of single-item life satisfaction measures. Results Consistent across three samples, single-item life satisfaction measures demonstrated substantial degree of criterion validity with the SWLS (zero-order r = 0.62 – 0.64; disattenuated r = 0.78 – 0.80). Patterns of statistical significance for correlations with theoretically relevant variables were the same across single-item measures and the SWLS. Single-item measures did not produce systematically different correlations compared to the SWLS (average difference = 0.001 – 0.005). The average absolute difference in the magnitudes of the correlations produced by single-item measures and the SWLS were very small (average absolute difference = 0.015 −0.042). Conclusions Single-item life satisfaction measures performed very similarly compared to the multiple-item SWLS. Social scientists would get virtually identical answer to substantive questions regardless of which measure they use. PMID:24890827

  3. Assessing the validity of single-item life satisfaction measures: results from three large samples.

    Science.gov (United States)

    Cheung, Felix; Lucas, Richard E

    2014-12-01

    The present paper assessed the validity of single-item life satisfaction measures by comparing single-item measures to the Satisfaction with Life Scale (SWLS)-a more psychometrically established measure. Two large samples from Washington (N = 13,064) and Oregon (N = 2,277) recruited by the Behavioral Risk Factor Surveillance System and a representative German sample (N = 1,312) recruited by the Germany Socio-Economic Panel were included in the present analyses. Single-item life satisfaction measures and the SWLS were correlated with theoretically relevant variables, such as demographics, subjective health, domain satisfaction, and affect. The correlations between the two life satisfaction measures and these variables were examined to assess the construct validity of single-item life satisfaction measures. Consistent across three samples, single-item life satisfaction measures demonstrated substantial degree of criterion validity with the SWLS (zero-order r = 0.62-0.64; disattenuated r = 0.78-0.80). Patterns of statistical significance for correlations with theoretically relevant variables were the same across single-item measures and the SWLS. Single-item measures did not produce systematically different correlations compared to the SWLS (average difference = 0.001-0.005). The average absolute difference in the magnitudes of the correlations produced by single-item measures and the SWLS was very small (average absolute difference = 0.015-0.042). Single-item life satisfaction measures performed very similarly compared to the multiple-item SWLS. Social scientists would get virtually identical answer to substantive questions regardless of which measure they use.

  4. Pronounceability: a measure of language samples based on children's mastery of the phonemes employed in them.

    Science.gov (United States)

    Whissell, Cynthia

    2003-06-01

    56 samples (n > half a million phonemes) of names (e.g., men's, women's jets'), song lyrics (e.g., Paul Simon's, rap, Beatles'), poems (frequently anthologized English poems), and children's materials (books directed at children ages 3-10 years) were used to study a proposed new measure of English language samples--Pronounceability-based on children's mastery of some phonemes in advance of others. This measure was provisionally equated with greater "youthfulness" and "playfulness" in language samples and with less "maturity." Findings include the facts that women's names were less pronounceable than men's and that poetry was less pronounceable than song lyrics or children's materials. In a supplementary study, 13 university student volunteers' assessments of the youth of randomly constructed names was linearly related to how pronounceable each name was (eta = .8), providing construct validity for the interpretation of Pronounceability as a measure of Youthfulness.

  5. Automation of the radiation measuring facilities for samples in health physics - MA 9

    International Nuclear Information System (INIS)

    Martini, M.

    1980-12-01

    Routine radation measurements of samples are performed by the HMI health physics department by means of test stations for individual samples and multiple samples (using a changing equipment). The basic device of these test stations is a SCALER/TIMER system (BF 22/25, BERTHOLD Corp.). This measuring facility has been extended by a CAMAC intrumentation which incorporates an autonomous CAMAC processor (CAPRO-1, INCAA B.V.) for monitoring an automatic control of the system. The programming language is BASIC. A DECwriter (LA 34) is used for user interaction and for printing the measurement results. This report describes the features of this system and present some examples of, the dialogue with the system and the printout of data. (orig.) [de

  6. Chemical Hygiene Plan for Onsite Measurement and Sample Shipping Facility Activities

    International Nuclear Information System (INIS)

    Price, W.H.

    1998-01-01

    This chemical hygiene plan presents the requirements established to ensure the protection of employee health while performing work in mobile laboratories, the sample shipping facility, and at the onsite radiological counting facility. This document presents the measures to be taken to promote safe work practices and to minimize worker exposure to hazardous chemicals. Specific hazardous chemicals present in the mobile laboratories, the sample shipping facility, and in the radiological counting facility are presented in Appendices A through G

  7. Identification of a Plasmid-Mediated Quinolone Resistance Gene in Salmonella Isolates from Texas Dairy Farm Environmental Samples.

    Science.gov (United States)

    Cummings, K J; Rodriguez-Rivera, L D; Norman, K N; Ohta, N; Scott, H M

    2017-06-01

    A recent increase in plasmid-mediated quinolone resistance (PMQR) has been detected among Salmonella isolated from humans in the United States, and it is necessary to determine the sources of human infection. We had previously isolated Salmonella from dairy farm environmental samples collected in Texas, and isolates were tested for anti-microbial susceptibility. Two isolates, serotyped as Salmonella Muenster, showed the discordant pattern of nalidixic acid susceptibility and intermediate susceptibility to ciprofloxacin. For this project, whole-genome sequencing of both isolates was performed to detect genes associated with quinolone resistance. The plasmid-mediated qnrB19 gene and IncR plasmid type were identified in both isolates. To our knowledge, this is the first report of PMQR in Salmonella isolated from food animals or agricultural environments in the United States. © 2016 Blackwell Verlag GmbH.

  8. Existing drug resistance among Staphylococcus spp. from raw milk samples in Khon Kaen province, Northeastern Thailand by direct quadriplex PCR

    Directory of Open Access Journals (Sweden)

    Buppachat Trakarnchan

    2017-10-01

    Full Text Available To describe the proportion of methicillin resistant Staphylococcus aureus, and mupirocin resistant among the isolates from milk, three hundred and eighty-one samples were collected in Khon Kaen province, Thailand, during January to March, 2014. Quadriplex PCR was a method of choice. The occurrence of S. aureus and other Staphylococcus spp. were 21.26 and 34.12%, respectively. Among the 81 S. aureus isolates, 82.72 (67/81, 11.11 (9/81, and 6.17% (5/81 were S. aureus, S. aureus carrying mecA, and S. aureus harboring mupA genes, respectively. These two mutant genes may possibly be transferred to other bacteria in milk. Therefore, good hygienic practices and strict control may limit the spread.

  9. A forward mutation assay using ampicillin-resistance in Escherichia coli designed for investigating the mutagenicity of biological samples.

    Science.gov (United States)

    Bosworth, D; Crofton-Sleigh, C; Venitt, S

    1987-11-01

    The development of a bacterial mutation assay using forward mutation to ampicillin-resistance is described. It is a technically simple assay using Escherichia coli D494uvrB transformed with a multi-copy mutator plasmid pGW1700. Mutation is detected by an increase in the frequency of ampicillin-resistant colonies following treatment of bacteria with the test material during logarithmic growth. The determination of viable counts allows a correction factor to be applied to compensate for the effects of sample-induced growth enhancement or toxicity on the bacterial population. The assay has been tested with a range of reference mutagens. It is particularly sensitive to base-pair substitution mutagens, detecting these at doses equal to or less than those detected in the Salmonella/microsome assay or the SOS Chromotest. The assay also detects frameshift mutagens but with lower sensitivity than the Salmonella/microsome assay.

  10. Electrical limit of silver nanowire electrodes: Direct measurement of the nanowire junction resistance

    KAUST Repository

    Selzer, Franz

    2016-04-19

    We measure basic network parameters of silvernanowire (AgNW) networks commonly used as transparent conductingelectrodes in organic optoelectronic devices. By means of four point probing with nanoprobes, the wire-to-wire junction resistance and the resistance of single nanowires are measured. The resistanceRNW of a single nanowire shows a value of RNW=(4.96±0.18) Ω/μm. The junction resistanceRJ differs for annealed and non-annealed NW networks, exhibiting values of RJ=(25.2±1.9) Ω (annealed) and RJ=(529±239) Ω (non-annealed), respectively. Our simulation achieves a good agreement between the measured network parameters and the sheet resistanceRS of the entire network. Extrapolating RJ to zero, our study show that we are close to the electrical limit of the conductivity of our AgNW system: We obtain a possible RS reduction by only ≈20% (common RS≈10 Ω/sq). Therefore, we expect further performance improvements in AgNW systems mainly by increasing NW length or by utilizing novel network geometries.

  11. Effect of measurement error budgets and hybrid metrology on qualification metrology sampling

    Science.gov (United States)

    Sendelbach, Matthew; Sarig, Niv; Wakamoto, Koichi; Kim, Hyang Kyun (Helen); Isbester, Paul; Asano, Masafumi; Matsuki, Kazuto; Osorio, Carmen; Archie, Chas

    2014-10-01

    Until now, metrologists had no statistics-based method to determine the sampling needed for an experiment before the start that accuracy experiment. We show a solution to this problem called inverse total measurement uncertainty (TMU) analysis, by presenting statistically based equations that allow the user to estimate the needed sampling after providing appropriate inputs, allowing him to make important "risk versus reward" sampling, cost, and equipment decisions. Application examples using experimental data from scatterometry and critical dimension scanning electron microscope tools are used first to demonstrate how the inverse TMU analysis methodology can be used to make intelligent sampling decisions and then to reveal why low sampling can lead to unstable and misleading results. One model is developed that can help experimenters minimize sampling costs. A second cost model reveals the inadequacy of some current sampling practices-and the enormous costs associated with sampling that provides reasonable levels of certainty in the result. We introduce the strategies on how to manage and mitigate these costs and begin the discussion on how fabs are able to manufacture devices using minimal reference sampling when qualifying metrology steps. Finally, the relationship between inverse TMU analysis and hybrid metrology is explored.

  12. Measurements of the Resistance of Main Circuit Busbar Segments at the LHC at Warm

    CERN Document Server

    Koratzinos, M; Flora, R H; Pfeffer, H; Scheuerlein, C; Siemko, A; Strait, J

    2010-01-01

    Following the QPS review in February 2009, a potential source of problems with the main circuit bus bar splices was identified: discontinuity in the copper stabilizer, coupled with lack of contact of the superconducting cable to the copper, in the vicinity of a splice. A campaign was launched to measure all such cases in the LHC. Non-invasive measurements were performed at room temperature or at about 80K in all sectors. A number of splices, following an indication obtained from these measurements, were opened and repaired. The resistance of these splices was also measured before and after the repair. We here present the results of this measuring campaign, together with the analysis that estimates the highest splice resistance left in the machine, which is a limiting factor to the maximum safe energy at which the LHC can operate

  13. Trapped field measurements on MgB{sub 2} bulk samples

    Energy Technology Data Exchange (ETDEWEB)

    Koblischka, Michael; Karwoth, Thomas; Zeng, XianLin; Hartmann, Uwe [Institute of Experimental Physics, Saarland University, P. O. Box 151150, D-66041 Saarbruecken (Germany); Berger, Kevin; Douine, Bruno [University of Lorraine, GREEN, 54506 Vandoeuvre-les-Nancy (France)

    2016-07-01

    Trapped field measurements were performed on bulk, polycrystalline MgB{sub 2} samples stemming from different sources with the emphasis to develop applications like superconducting permanent magnets ('supermagnets') and electric motors. We describe the setup for the trapped field measurements and the experimental procedure (field cooling, zero-field cooling, field sweep rates). The trapped field measurements were conducted using a cryocooling system to cool the bulk samples to the desired temperatures, and a low-loss cryostat equipped with a room-temperature bore and a maximum field of ±5 T was employed to provide the external magnetic field. The superconducting coil of this cryostat is operated using a bidirectional power supply. Various sweep rates of the external magnetic field ranging between 1 mT/s and 40 mT/s were used to generate the applied field. The measurements were performed with one sample and two samples stacked together. A maximum trapped field of 7 T was recorded. We discuss the results obtained and the problems arising due to flux jumping, which is often seen for the MgB{sub 2} samples cooled to temperatures below 10 K.

  14. Relations among questionnaire and experience sampling measures of inner speech: A smartphone app study

    Directory of Open Access Journals (Sweden)

    Ben eAlderson-Day

    2015-04-01

    Full Text Available Inner speech is often reported to be a common and central part of inner experience, but its true prevalence is unclear. Many questionnaire-based measures appear to lack convergent validity and it has been claimed that they overestimate inner speech in comparison to experience sampling methods (which involve collecting data at random timepoints. The present study compared self-reporting of inner speech collected via a general questionnaire and experience sampling, using data from a custom-made smartphone app (Inner Life. Fifty-one university students completed a generalized self-report measure of inner speech (the Varieties of Inner Speech Questionnaire, or VISQ and responded to at least 7 random alerts to report on incidences of inner speech over a 2-week period. Correlations and pairwise comparisons were used to compare generalized endorsements and randomly-sampled scores for each VISQ subscale. Significant correlations were observed between general and randomly sampled measures for only 2 of the 4 VISQ subscales, and endorsements of inner speech with evaluative or motivational characteristics did not correlate at all across different measures. Endorsement of inner speech items was significantly lower for random sampling compared to generalized self-report, for all VISQ subscales. Exploratory analysis indicated that specific inner speech characteristics were also related to anxiety and future-oriented thinking.

  15. Calorimeters for Precision Power Dissipation Measurements on Controlled-Temperature Superconducting Radiofrequency Samples

    International Nuclear Information System (INIS)

    Xiao, Binping P.; Kelley, Michael J.; Reece, Charles E.; Phillips, H. L.

    2012-01-01

    Two calorimeters, with stainless steel and Cu as the thermal path material for high precision and high power versions, respectively, have been designed and commissioned for the surface impedance characterization (SIC) system at Jefferson Lab to provide low temperature control and measurement for CW power up to 22 W on a 5 cm dia. disk sample which is thermally isolated from the RF portion of the system. A power compensation method has been developed to measure the RF induced power on the sample. Simulation and experimental results show that with these two calorimeters, the whole thermal range of interest for superconducting radiofrequency (SRF) materials has been covered. The power measurement error in the interested power range is within 1.2% and 2.7% for the high precision and high power versions, respectively. Temperature distributions on the sample surface for both versions have been simulated and the accuracy of sample temperature measurements have been analysed. Both versions have the ability to accept bulk superconductors and thin film superconducting samples with a variety of substrate materials such as Al, Al 2 O 3 , Cu, MgO, Nb and Si

  16. Measurement of radon gas concentration in fertilizer samples by using nuclear track detector (CR-39)

    International Nuclear Information System (INIS)

    Karim, M.S.; Abbas, F.I.; Mnawer, E.M.; Abbas, K.H.

    2011-01-01

    In the present work, we have measured the radon gas concentration in ten fertilizers samples for three kinds { mixed fertilizer (M.F), triple super phosphate (T.S.P), single super phosphate (S.S.P) } by using alpha-emitters registrations which are emitted form radon gas in (CR-39) nuclear track detector.The results obtained have shown that the highest average radon gas concentration in fertilizer samples was found in triple super phosphate sample(T.S.P.), which was (177.5Bq/m 3 ) (Iran origin), while the lowest average radon gas concentration was found in single super phosphate (S.S.P.) sample, which was (72.2Bq/m 3 ) (Iraq origin).The present results show that the radon gas concentration in all fertilizers samples is below the allowed limit from (International Commission of Radiation Protection) (ICRP) agency

  17. Intercomparison of radionuclide measurements in marine fish flesh sample MA-B-3

    International Nuclear Information System (INIS)

    1987-08-01

    This final report presents results of the laboratory intercomparison of the activity concentration determination of 40 K, 90 Sr, 137 Cs, 210 Pb, 210 Po, 226 Ra, 228 Th, 230 Th, 232 Th, 238 U, 239 Pu, 240 Pu and 241 Am in the sample of garpike fish organized by the IAEA's Analytical Quality Control Service. The forty-three laboratories from twenty-three countries have reported results established by different analytical techniques. This sample is intended as a reference material for the measurement of 40 K and 137 Cs in marine biological samples and other similar matrices. 4 refs, 9 tabs

  18. Comparison of three methods of sampling trout blood for measurements of hematocrit

    Science.gov (United States)

    Steucke, Erwin W.; Schoettger, Richard A.

    1967-01-01

    Trout blood is frequently collected for hematocrit measurements by excising the caudal fin (Snieszko, 1960), but this technique is impractical if valuable fish are to be sampled or if repeated observations are desired. Schiffman (1959) and Snieszko (1960) collected blood from the dorsal aorta and the heart, but these methods are relatively slow and require the preparation of needles and syringes. The use of pointed capillary tubes for cardiac punctures increases the speed of sampling, but body fluids may dilute the blood (Perkins, 1957; Larsen and Snieszko, 1961; and Normandau, 1962). There is need for methods of sampling which are rapid and which neither influence hematological determinations nor harm the fish.

  19. Design of a horizontal penetrometer for measuring on-the-go soil resistance.

    Science.gov (United States)

    Topakci, Mehmet; Unal, Ilker; Canakci, Murad; Celik, Huseyin Kursat; Karayel, Davut

    2010-01-01

    Soil compaction is one of the main negative factors that limits plant growth and crop yield. Therefore, it is important to determine the soil resistance level and map it for the field to find solutions for the negative effects of the compaction. Nowadays, high powered communication technology and computers help us on this issue within the approach of precision agriculture applications. This study is focused on the design of a penetrometer, which can make instantaneous soil resistance measurements in the soil horizontally and data acquisition software based on the GPS (Global Positioning System). The penetrometer was designed using commercial 3D parametric solid modelling design software. The data acquisition software was developed in Microsoft Visual Basic.NET programming language. After the design of the system, manufacturing and assembly of the system was completed and then a field experiment was carried out. According to the data from GPS and penetration resistance values which are collected in Microsoft SQL Server database, a Kriging method by ArcGIS was used and soil resistance was mapped in the field for a soil depth of 40 cm. During operation, no faults, either in mechanical and software parts, were seen. As a result, soil resistance values of 0.2 MPa and 3 MPa were obtained as minimum and maximum values, respectively. In conclusion, the experimental results showed that the designed system works quite well in the field and the horizontal penetrometer is a practical tool for providing on-line soil resistance measurements. This study contributes to further research for the development of on-line soil resistance measurements and mapping within the precision agriculture applications.

  20. Design of a Horizontal Penetrometer for Measuring On‑the‑Go Soil Resistance

    Directory of Open Access Journals (Sweden)

    Davut Karayel

    2010-10-01

    Full Text Available Soil compaction is one of the main negative factors that limits plant growth and crop yield. Therefore, it is important to determine the soil resistance level and map it for the field to find solutions for the negative effects of the compaction. Nowadays, high powered communication technology and computers help us on this issue within the approach of precision agriculture applications. This study is focused on the design of a penetrometer, which can make instantaneous soil resistance measurements in the soil horizontally and data acquisition software based on the GPS (Global Positioning System. The penetrometer was designed using commercial 3D parametric solid modelling design software. The data acquisition software was developed in Microsoft Visual Basic.NET programming language. After the design of the system, manufacturing and assembly of the system was completed and then a field experiment was carried out. According to the data from GPS and penetration resistance values which are collected in Microsoft SQL Server database, a Kriging method by ArcGIS was used and soil resistance was mapped in the field for a soil depth of 40 cm. During operation, no faults, either in mechanical and software parts, were seen. As a result, soil resistance values of 0.2 MPa and 3 MPa were obtained as minimum and maximum values, respectively. In conclusion, the experimental results showed that the designed system works quite well in the field and the horizontal penetrometer is a practical tool for providing on‑line soil resistance measurements. This study contributes to further research for the development of on-line soil resistance measurements and mapping within the precision agriculture applications.

  1. Studies on the true coincidence correction in measuring filter samples by gamma spectrometry

    CERN Document Server

    Lian Qi; Chang Yong Fu; Xia Bing

    2002-01-01

    The true coincidence correction in measuring filter samples has been studied by high efficiency HPGe gamma detectors. The true coincidence correction for a specific three excited levels de-excitation case has been analyzed, and the typical analytical expressions of true coincidence correction factors have been given. According to the measured relative efficiency on the detector surface with 8 'single' energy gamma emitters and efficiency of filter samples, the peak and total efficiency surfaces are fitted. The true coincidence correction factors of sup 6 sup 0 Co and sup 1 sup 5 sup 2 Eu calculated by the efficiency surfaces agree well with experimental results

  2. Rapid Extraction from and Direct Identification in Clinical Samples of Methicillin-Resistant Staphylococci Using the PCR

    Science.gov (United States)

    Jaffe, Richard I.; Lane, Janae D.; Albury, Stephen V.; Niemeyer, Debra M.

    2000-01-01

    Methicillin-resistant staphylococci (MRS) are one of the most common causes of nosocomial infections and bacteremia. Standard bacterial identification and susceptibility testing frequently require as long as 72 h to report results, and there may be difficulty in rapidly and accurately identifying methicillin resistance. The use of the PCR is a rapid and simple process for the amplification of target DNA sequences, which can be used to identify and test bacteria for antimicrobial resistance. However, many sample preparation methods are unsuitable for PCR utilization in the clinical laboratory because they either are not cost-effective, take too long to perform, or do not provide a satisfactory DNA template for PCR. Our goal was to provide same-day results to facilitate rapid diagnosis and therapy. In this report, we describe a rapid method for extraction of bacterial DNA directly from blood culture bottles that gave quality DNA for PCR in as little as 20 min. We compared this extraction method to the standard QIAGEN method for turnaround time (TAT), cost, purity, and use of template in PCR. Specific identification of MRS was determined using intragenic primer sets for bacterial and Staphylococcus 16S rRNA and mecA gene sequences. The PCR primer sets were validated with 416 isolates of staphylococci, including methicillin-resistant Staphylococcus aureus (n = 106), methicillin-sensitive S. aureus (n = 134), and coagulase-negative Staphylococcus (n = 176). The total supply cost of our extraction method and PCR was $2.15 per sample with a result TAT of less than 4 h. The methods described herein represent a rapid and accurate DNA extraction and PCR-based identification system, which makes the system an ideal candidate for use under austere field conditions and one that may have utility in the clinical laboratory. PMID:10970392

  3. A portable molecular-sieve-based CO2 sampling system for radiocarbon measurements.

    Science.gov (United States)

    Palonen, V

    2015-12-01

    We have developed a field-capable sampling system for the collection of CO2 samples for radiocarbon-concentration measurements. Most target systems in environmental research are limited in volume and CO2 concentration, making conventional flask sampling hard or impossible for radiocarbon studies. The present system captures the CO2 selectively to cartridges containing 13X molecular sieve material. The sampling does not introduce significant under-pressures or significant losses of moisture to the target system, making it suitable for most environmental targets. The system also incorporates a significantly larger sieve container for the removal of CO2 from chambers prior to the CO2 build-up phase and sampling. In addition, both the CO2 and H2O content of the sample gas are measured continuously. This enables in situ estimation of the amount of collected CO2 and the determination of CO2 flux to a chamber. The portable sampling system is described in detail and tests for the reliability of the method are presented.

  4. Identification and estimation of nonlinear models using two samples with nonclassical measurement errors

    KAUST Repository

    Carroll, Raymond J.

    2010-05-01

    This paper considers identification and estimation of a general nonlinear Errors-in-Variables (EIV) model using two samples. Both samples consist of a dependent variable, some error-free covariates, and an error-prone covariate, for which the measurement error has unknown distribution and could be arbitrarily correlated with the latent true values; and neither sample contains an accurate measurement of the corresponding true variable. We assume that the regression model of interest - the conditional distribution of the dependent variable given the latent true covariate and the error-free covariates - is the same in both samples, but the distributions of the latent true covariates vary with observed error-free discrete covariates. We first show that the general latent nonlinear model is nonparametrically identified using the two samples when both could have nonclassical errors, without either instrumental variables or independence between the two samples. When the two samples are independent and the nonlinear regression model is parameterized, we propose sieve Quasi Maximum Likelihood Estimation (Q-MLE) for the parameter of interest, and establish its root-n consistency and asymptotic normality under possible misspecification, and its semiparametric efficiency under correct specification, with easily estimated standard errors. A Monte Carlo simulation and a data application are presented to show the power of the approach.

  5. On the optimal sampling of bandpass measurement signals through data acquisition systems

    International Nuclear Information System (INIS)

    Angrisani, L; Vadursi, M

    2008-01-01

    Data acquisition systems (DAS) play a fundamental role in a lot of modern measurement solutions. One of the parameters characterizing a DAS is its maximum sample rate, which imposes constraints on the signals that can be alias-free digitized. Bandpass sampling theory singles out separated ranges of admissible sample rates, which can be significantly lower than carrier frequency. But, how to choose the most convenient sample rate according to the purpose at hand? The paper proposes a method for the automatic selection of the optimal sample rate in measurement applications involving bandpass signals; the effects of sample clock instability and limited resolution are also taken into account. The method allows the user to choose the location of spectral replicas of the sampled signal in terms of normalized frequency, and the minimum guard band between replicas, thus introducing a feature that no DAS currently available on the market seems to offer. A number of experimental tests on bandpass digitally modulated signals are carried out to assess the concurrence of the obtained central frequency with the expected one

  6. Advancing Research on Racial–Ethnic Health Disparities: Improving Measurement Equivalence in Studies with Diverse Samples

    Science.gov (United States)

    Landrine, Hope; Corral, Irma

    2014-01-01

    To conduct meaningful, epidemiologic research on racial–ethnic health disparities, racial–ethnic samples must be rendered equivalent on other social status and contextual variables via statistical controls of those extraneous factors. The racial–ethnic groups must also be equally familiar with and have similar responses to the methods and measures used to collect health data, must have equal opportunity to participate in the research, and must be equally representative of their respective populations. In the absence of such measurement equivalence, studies of racial–ethnic health disparities are confounded by a plethora of unmeasured, uncontrolled correlates of race–ethnicity. Those correlates render the samples, methods, and measures incomparable across racial–ethnic groups, and diminish the ability to attribute health differences discovered to race–ethnicity vs. to its correlates. This paper reviews the non-equivalent yet normative samples, methodologies and measures used in epidemiologic studies of racial–ethnic health disparities, and provides concrete suggestions for improving sample, method, and scalar measurement equivalence. PMID:25566524

  7. Measurement of the tritium concentration in the fractionated distillate from environmental water samples.

    Science.gov (United States)

    Atkinson, Robert; Eddy, Teresa; Kuhne, Wendy; Jannik, Tim; Brandl, Alexander

    2014-09-01

    Standard procedures for the measurement of tritium in water samples often require distillation of an appropriate sample aliquot. This distillation process may result in a fractionation of tritiated water and regular light water due to the vapor pressure isotope effect, introducing either a bias or an additional contribution to the total tritium measurement uncertainty. The current study investigates the relative change in vapor pressure isotope effect in the course of the distillation process, distinguishing it from and extending previously published measurements. The separation factor as a quantitative measure of the vapor pressure isotope effect is found to assume values of 1.04 ± 0.036, 1.05 ± 0.026, and 1.07 ± 0.038, depending on the vigor of the boiling process during distillation of the sample. A lower heat setting in the experimental setup, and therefore a less vigorous boiling process, results in a larger value for the separation factor. For a tritium measurement in water samples where the first 5 mL are discarded, the tritium concentration could be underestimated by 4-7%. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Pressurizer level measurement inside PWR nuclear plant using resistance type heat sensors

    International Nuclear Information System (INIS)

    El Moussaoui, Ahmed.

    1982-06-01

    The accident that occured in 1979 to the PWR type nuclear reactor, Three-Mile Island 2, has drawn attention to the maladjustement of the differentiel pressure level detector installed in nuclear plants on the market. A system is presented here for measuring the level in pressurizers based on measurements of the heat resistance of the boundary layer existing between the heated sensor and the fluid mass in the vessel. The sensor consists of a 3 cm diameter cylindrical insulator support around which a 0.1 mm diameter platinum filament is wound. This filament simultaneously fulfills heating and transducer functions. To verify the feasibility of the resistant type heat sensor a test system, which provides water and steam under pressure was realised. Static and dynamic tests have shown that the principle of the resistant heat sensor is viable and can be used to obtain level informations [fr

  9. Pedestrian fall safety assessments improved understanding on slip resistance measurements and investigations

    CERN Document Server

    Kim, In-Ju

    2017-01-01

    This book examines pedestrian shoe-floor slip resistance from an engineering standpoint in order to better understand friction and wear behavior. This analysis includes an extensive investigation into the surface properties of shoes and flow, and the measurement of dynamic friction and other mechanical and physical aspects of shoe-floor tribology. Lastly, the book proposes a measurement concept for the identification and classification of operational floor surfaces under a range of different conditions. Novel techniques and methods are proposed that can improve the reliability of slip resistance assessments. The current state of knowledge is critically examined and discussed from a tribological perspective, including aspects like friction, wear, lubrication and the mechanical behavior of shoes, floors and their wider environment. Further, the book reports on extensive experimental investigations into the topographical characteristics of shoe and floor surfaces and how they affect slip resistance. Slips result...

  10. High Resistance to Azithromycin in Clinical Samples from Patients with Sexually Transmitted Diseases in Guangxi Zhuang Autonomous Region, China.

    Science.gov (United States)

    Zhu, Bangyong; Bu, Jin; Li, Wei; Zhang, Jie; Huang, Geng; Cao, Juan; Tang, Zhongshu; Gan, Quan; Wei, Pingjiang

    2016-01-01

    Azithromycin is used as an alternative medicine in patients with syphilis who are intolerant to penicillin. Nevertheless, the report of treatment failure of azithromycin for patients with syphilis has raised concerns in China in the past years. In this study, 178 patients with early syphilis, who were treated in sexually transmitted infections clinics in four cities in Guangxi Zhuang Autonomous Region were enrolled to investigate the regional prevalence of Treponema pallidum strain resistant to azithromycin. Nested PCR was performed to amplify the 23S ribosomal RNA (23SrRNA) gene. The point mutation of A2058G in 23SrRNA, which confers Treponema pallidum resistance to azithromycin, was measured by endonuclease digestion of PCR amplification products using MboII. A2058G point mutation was detected in 91.0% (162/178; 95% CI, 86.8%, 95.2%) of the specimens, but no difference in prevalence of azithromycin resistance was found between the patients who had taken antibiotics before enrollment and the patients who had not (91.8% vs. 89.4%), nor between the patients with and without past sexually transmitted infections (87.1% vs. 93.1%). We concluded that azithromycin may not be suitable for syphilis as a treatment option in Guangxi Zhuang Autonomous Region because of the extremely high prevalence of resistance in the general syphilis population.

  11. Diagnostic accuracy of language sample measures with Persian-speaking preschool children.

    Science.gov (United States)

    Kazemi, Yalda; Klee, Thomas; Stringer, Helen

    2015-04-01

    This study examined the diagnostic accuracy of selected language sample measures (LSMs) with Persian-speaking children. A pre-accuracy study followed by phase I and II studies are reported. Twenty-four Persian-speaking children, aged 42 to 54 months, with primary language impairment (PLI) were compared to 27 age-matched children without PLI on a set of measures derived from play-based, conversational language samples. Results showed that correlations between age and LSMs were not statistically significant in either group of children. However, a majority of LSMs differentiated children with and without PLI at the group level (phase I), while three of the measures exhibited good diagnostic accuracy at the level of the individual (phase II). We conclude that general LSMs are promising for distinguishing between children with and without PLI. Persian-specific measures are mainly helpful in identifying children without language impairment while their ability to identify children with PLI is poor.

  12. Measurement and Comparison of Organic Compound Concentrations in Plasma, Whole Blood and Dried Blood Spot Samples

    Directory of Open Access Journals (Sweden)

    Stuart A Batterman

    2016-04-01

    Full Text Available The preferred sampling medium for measuring human exposures of persistent organic compounds (POPs is blood, and relevant sample types include whole blood, plasma, and dried blood spots (DBS. Because information regarding the performance and comparability of measurements across these sample types is limited, it is difficult to compare across studies. This study evaluates the performance of POP measurements in plasma, whole blood and DBS, and presents the distribution coefficients needed to convert concentrations among the three sample types. Blood samples were collected from adult volunteers, along with demographic and smoking information, and analyzed by GC/MS for organochlorine pesticides (OCPs, chlorinated hydrocarbons (CHCs, polychlorinated biphenyls (PCBs, and brominated diphenyl ethers (PBDEs. Regression models were used to evaluate the relationships between the sample types and possible effects of personal covariates. Distribution coefficients also were calculated using physically-based models.Across all compounds, concentrations in plasma were consistently the highest; concentrations in whole blood and DBS samples were comparable. Distribution coefficients for plasma to whole blood concentrations ranged from 1.74 to 2.26 for pesticides/CHCs, averaged 1.69 ± 0.06 for the PCBs, and averaged 1.65 ± 0.03 for the PBDEs. Regression models closely fit most chemicals (R2 > 0.80, and whole blood and DBS samples generally showed very good agreement. Distribution coefficients estimated using biologically-based models were near one and did not explain the observed distribution. Among the study population, median concentrations of several pesticides/CHCs and PBDEs exceeded levels reported in the 2007-2008 National Health and Nutrition Examination Survey, while levels of other OCPs and PBDEs were comparable or lower. Race and smoking status appeared to slightly affect plasma/blood concentration ratios for several POPs. The experimentally

  13. Measuring Biometric Sample Quality in terms of Biometric Feature Information in Iris Images

    OpenAIRE

    Youmaran, R.; Adler, A.

    2012-01-01

    This paper develops an approach to measure the information content in a biometric feature representation of iris images. In this context, the biometric feature information is calculated using the relative entropy between the intraclass and interclass feature distributions. The collected data is regularized using a Gaussian model of the feature covariances in order to practically measure the biometric information with limited data samples. An example of this method is shown for iris templates ...

  14. Monte Carlo simulation of air sampling methods for the measurement of radon decay products.

    Science.gov (United States)

    Sima, Octavian; Luca, Aurelian; Sahagia, Maria

    2017-08-01

    A stochastic model of the processes involved in the measurement of the activity of the 222 Rn decay products was developed. The distributions of the relevant factors, including air sampling and radionuclide collection, are propagated using Monte Carlo simulation to the final distribution of the measurement results. The uncertainties of the 222 Rn decay products concentrations in the air are realistically evaluated. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Design of a radioactive gas sampling system for NESHAP compliance measurements of 41Ar

    International Nuclear Information System (INIS)

    Newton, G.J.; McDonald, M.J.; Ghanbari, F.; Hoover, M.D.; Barr, E.B.

    1994-01-01

    United States Department of Energy facilities are required to comply with the U.S. Environmental Protection Agency, National Emission Standard for Hazardous Air Pollutants (NESHAP) 40 CFR, part 61, subpart H. Compliance generally requires confirmatory measurements of emitted radionuclides. Although a number of standard procedures exist for extractive sampling of particle-associated radionuclides, sampling approaches for radioactive gases are less defined. Real-time, flow-through sampling of radioactive gases can be done when concentrations are high compared to interferences from background radiation. Cold traps can be used to collect and concentrate condensible effluents in applications where cryogenic conditions can be established and maintained. Commercially available gas-sampling cylinders can be used to capture grab samples of contaminated air under ambient or compressed conditions, if suitable sampling and control hardware are added to the cylinders. The purpose of the current study was to develop an efficient and compact set of sampling and control hardware for use with commercially available gas-sampling cylinders, and to demonstrate its use in NESHAP compliance testing of 41 Ar at two experimental research reactors

  16. Measurement of biochemical oxygen demand from different wastewater samples using a mediator-less microbial fuel cell biosensor.

    Science.gov (United States)

    Hsieh, Min-Chi; Chung, Ying-Chien

    2014-01-01

    Microbial fuel cells (MFCs) have attracted considerable attention as potential biosensors. A MFC biosensor for rapid measurement of biochemical oxygen demand (BOD) has been recently studied. However, a standardized bacterial mixture inoculated in the MFC biosensor for BOD measurement is unavailable. Thus, the commercial application of a MFC biosensor is limited. In this study, a mediator-less MFC biosensor inoculated with known mixed cultures to quickly determine BOD concentration was tested. Optimal external resistance, operating temperature and measurement time for the MFC biosensor were determined to be 5000 omega, 35 degrees C and 12h, respectively. A good relationship between BOD concentration and voltage output, high reproducibility and long-term stability for the MFC biosensor was observed. The newly developed MFC biosensor was inoculated with a mixture of six bacterial strains (Thermincola carboxydiphila, Pseudomonas aeruginosa, Ochrobactrum intermedium, Shewanella frigidimarina, Citrobacter freundii and Clostridium acetobutylicum) capable of degrading complex organic compounds and surviving toxic conditions. The described MFC biosensor was able to successfully measure BOD concentrations below 240 mg L(-1) in real wastewater samples.

  17. Changes in iron measures over menopause and associations with insulin resistance.

    Science.gov (United States)

    Kim, Catherine; Nan, Bin; Kong, Shengchun; Harlow, Siobhan

    2012-08-01

    No longitudinal studies have examined how iron measures change over menopause. Our objectives were to examine iron measures in individual women at premenopause and at postmenopause and, secondarily, to determine if any changes contributed to insulin resistance. In a subset of participants (n=70) in a longitudinal study of menopause, we measured ferritin, transferrin, and soluble transferrin receptor (sTfR) once in the premenopause and once in the postmenopause. We also examined associations between menopausal status and change in iron markers after adjustment for age at menopause, race/ethnicity, and waist circumference. In linear regression models, we examined associations between premenopause iron measures and changes in iron markers over menopause with homeostasis model assessment of insulin resistance (HOMA-IR) changes over menopause, before and after adjustment for age at menopause, race/ethnicity, changes in waist circumference, C-reactive protein (CRP), and sex hormone-binding globulin (SHBG) levels. Women had lower ferritin (pmenopause (changes in sTfR:ferritin β=13.6, 95% CI 0.93-26.3) were associated with larger increases in HOMA-IR. From premenopause to postmenopause, women on average have increases in measures of iron stores. Women who had the greatest changes in iron over menopause (lower measures of premenopausal iron and greater increases in iron measures over the menopause) had the strongest associations between changes in iron and changes in insulin resistance.

  18. Comparison between Laboratory Measurements, Simulations and Analytical Predictions of the Resistive Wall Transverse Beam Impedance at low frequencies

    CERN Document Server

    Roncarolo, F; Kroyer, T; Métral, E; Salvant, B

    2008-01-01

    The prediction of the resistive wall transverse beam impedance at the first unstable betatron line (8 kHz) of the CERN Large Hadron Collider (LHC) is of paramount importance for understanding and controlling the related coupled-bunch instability. Until now only novel analytical formulas were available at this frequency. Recently, laboratory measurements and numerical simulations were performed to crosscheck the analytical predictions. The experimental results based on the measurement of the variation of a probe coil inductance in the presence of i) sample graphite plates, ii) stand-alone LHC collimator jaws and iii) a full LHC collimator assembly are presented in detail. The measurement results are compared to both analytical theories and simulations. In addition, the consequences for the understanding of the LHC impedance are discussed.

  19. Impact of sampling techniques on measured stormwater quality data for small streams

    Science.gov (United States)

    Harmel, R.D.; Slade, R.M.; Haney, R.L.

    2010-01-01

    Science-based sampling methodologies are needed to enhance water quality characterization for setting appropriate water quality standards, developing Total Maximum Daily Loads, and managing nonpoint source pollution. Storm event sampling, which is vital for adequate assessment of water quality in small (wadeable) streams, is typically conducted by manual grab or integrated sampling or with an automated sampler. Although it is typically assumed that samples from a single point adequately represent mean cross-sectional concentrations, especially for dissolved constituents, this assumption of well-mixed conditions has received limited evaluation. Similarly, the impact of temporal (within-storm) concentration variability is rarely considered. Therefore, this study evaluated differences in stormwater quality measured in small streams with several common sampling techniques, which in essence evaluated within-channel and within-storm concentration variability. Constituent concentrations from manual grab samples and from integrated samples were compared for 31 events, then concentrations were also compared for seven events with automated sample collection. Comparison of sampling techniques indicated varying degrees of concentration variability within channel cross sections for both dissolved and particulate constituents, which is contrary to common assumptions of substantial variability in particulate concentrations and of minimal variability in dissolved concentrations. Results also indicated the potential for substantial within-storm (temporal) concentration variability for both dissolved and particulate constituents. Thus, failing to account for potential cross-sectional and temporal concentration variability in stormwater monitoring projects can introduce additional uncertainty in measured water quality data. Copyright ?? 2010 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. All rights reserved.

  20. Impact of shrinking measurement error budgets on qualification metrology sampling and cost

    Science.gov (United States)

    Sendelbach, Matthew; Sarig, Niv; Wakamoto, Koichi; Kim, Hyang Kyun (Helen); Isbester, Paul; Asano, Masafumi; Matsuki, Kazuto; Vaid, Alok; Osorio, Carmen; Archie, Chas

    2014-04-01

    When designing an experiment to assess the accuracy of a tool as compared to a reference tool, semiconductor metrologists are often confronted with the situation that they must decide on the sampling strategy before the measurements begin. This decision is usually based largely on the previous experience of the metrologist and the available resources, and not on the statistics that are needed to achieve acceptable confidence limits on the final result. This paper shows a solution to this problem, called inverse TMU analysis, by presenting statistically-based equations that allow the user to estimate the needed sampling after providing appropriate inputs, allowing him to make important "risk vs. reward" sampling, cost, and equipment decisions. Application examples using experimental data from scatterometry and critical dimension scanning electron microscope (CD-SEM) tools are used first to demonstrate how the inverse TMU analysis methodology can be used to make intelligent sampling decisions before the start of the experiment, and then to reveal why low sampling can lead to unstable and misleading results. A model is developed that can help an experimenter minimize the costs associated both with increased sampling and with making wrong decisions caused by insufficient sampling. A second cost model is described that reveals the inadequacy of current TEM (Transmission Electron Microscopy) sampling practices and the enormous costs associated with TEM sampling that is needed to provide reasonable levels of certainty in the result. These high costs reach into the tens of millions of dollars for TEM reference metrology as the measurement error budgets reach angstrom levels. The paper concludes with strategies on how to manage and mitigate these costs.

  1. Assessing the validity of parenting measures in a sample of chinese adolescents.

    Science.gov (United States)

    Supple, Andrew J; Peterson, Gary W; Bush, Kevin R

    2004-09-01

    The purpose of this study was to assess the construct validity of adolescent-report parenting behavior measures (primarily derived from the Parental Behavior Measure) in a sample of 480 adolescents from Beijing, China. Results suggest that maternal support, monitoring, and autonomy granting were valid measures when assessing maternal socialization strategies and Chinese adolescent development. Measures of punitiveness and love withdrawal demonstrated limited validity, whereas maternal positive induction demonstrated little validity. The major implications of these results are that measures of "negative" parenting that included physical or psychological manipulations may not have salience for the development of Chinese adolescents. Moreover, researchers and clinicians should question the applicability of instruments and measures designed to assess family process when working with individuals in families from diverse cultural backgrounds. Copyright 2004 American Psychological Association

  2. A study of cross-bridge kelvin resistor structures for reliable measurement of low contact resistances

    NARCIS (Netherlands)

    Stavitski, N.; Klootwijk, J.H.; van Zeijl, H.W.; Kovalgin, Alexeij Y.; Wolters, Robertus A.M.

    2008-01-01

    The parasitic factors that strongly influence the measurement accuracy of Cross-Bridge Kelvin Resistor (CBKR) structures for low specific contact resistances (�?c) have been extensively discussed during last few decades and the minimum of the �?c value, which could be accurately extracted, was

  3. Sensitivity of surface resistance measurement of HTS thin films by ...

    Indian Academy of Sciences (India)

    Sensitivity of surface resistance measurement of HTS thin films by cavity resonator, dielectric resonator and microstrip line resonator ... 012, India; Research Center for Superconductor Photonics, Osaka University, Osaka 565 0871, Japan; Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India ...

  4. Ion beam mixing of FeAl multilayers: in-situ kinetics by resistivity measurements

    International Nuclear Information System (INIS)

    Riviere, J.P.; Delafond, J.; Jaouen, C.; Bellara, A.; Dinhu, J.F.

    1984-01-01

    Electrical resistivity measurements performed continuously in situ during ion implantations are used to study the ion beam mixing of FeAl multilayers. The total mixing of the films is easily determined from the experiments, and the dose variations of the volume fraction of mixed atoms is also deduced [fr

  5. Dispositional resistance to change: Measurement equivalence and the link to personal values across 17 nations

    Czech Academy of Sciences Publication Activity Database

    Oreg, S.; Bayazit, M.; Vakola, M.; Arciniega, L.; Armenakis, A.; Barkauskiene, R.; Bozionelos, N.; Ferič, I.; Fujimoto, Y.; Gonzáles, L.; Han, J.; Hetland, H.; Hřebíčková, Martina; Jimmieson, N.; Kordačová, J.; Kotrla Topič, M.; Mitsuhashi, H.; Mlacić, B.; Ohly, S.; Saksvik, I.; Saksvik, P.O.; van Dam, K.

    2008-01-01

    Roč. 93, č. 4 (2008), s. 935-944 ISSN 0021-9010 R&D Projects: GA AV ČR IAA700250702 Institutional research plan: CEZ:AV0Z70250504 Keywords : resistance to change * personal values * measurement equivalence Subject RIV: AN - Psychology Impact factor: 3.769, year: 2008

  6. Measuring the thermal insulation and evaporative resistance of sleeping bags using a supine sweating fabric manikin

    International Nuclear Information System (INIS)

    Wu, Y S; Fan, Jintu

    2009-01-01

    For testing the thermal insulation of sleeping bags, standard test methods and procedures using heated manikins are provided in ASTM F1720-06 and EN 13537:2002. However, with regard to the evaporative resistance of sleeping bags, no instrument or test method has so far been established to give a direct measurement. In this paper, we report on a novel supine sweating fabric manikin system for directly measuring the evaporative resistance of sleeping bags. Eleven sleeping bags were tested using the manikin under the isothermal condition, namely, both the mean skin temperature of the manikin and that of the environment were controlled to be the same at 35 °C, with the wind speed and ambient relative humidity at 0.3 m s −1 and 50%, respectively. The results showed that the novel supine sweating fabric manikin is reproducible and accurate in directly measuring the evaporative resistance of sleeping bags, and the measured evaporative resistance can be combined with thermal insulation to calculate the moisture permeability index of sleeping bags

  7. Evaluating parameterizations of aerodynamic resistance to heat transfer using field measurements

    NARCIS (Netherlands)

    Liu, S.; Lu, L.; Mao, D.; Jia, L.

    2007-01-01

    Parameterizations of aerodynamic resistance to heat and water transfer have a significant impact on the accuracy of models of land - atmosphere interactions and of estimated surface fluxes using spectro-radiometric data collected from aircrafts and satellites. We have used measurements from an eddy

  8. Test-Retest Reliability of Respiratory Resistance Measured with the Airflow Perturbation Device

    Science.gov (United States)

    Gallena, Sally K.; Solomon, Nancy Pearl; Johnson, Arthur T.; Vossoughi, Jafar; Tian, Wei

    2014-01-01

    Purpose: In this study, the authors aimed to determine reliability of the airflow perturbation device (APD) to measure respiratory resistance within and across sessions during resting tidal (RTB) and postexercise breathing in healthy athletes, and during RTB across trials within a session in athletes with paradoxical vocal fold motion (PVFM)…

  9. Antibiotic resistance profile of bacteria isolated from raw milk samples of cattle and buffaloes

    Directory of Open Access Journals (Sweden)

    Tahlina Tanzin

    2016-03-01

    Conclusion: Two different species of bacteria i.e., S. aureus and E. coli are contaminating with milk samples. The pathogenic bacteria can be controlled effectively by using Ciprofloxacin and Levofloxacin in the case of mastitis in cattle and buffaloes in Bangladesh. [J Adv Vet Anim Res 2016; 3(1.000: 62-67

  10. A simplified method for low-level tritium measurement in the environmental water samples

    International Nuclear Information System (INIS)

    Sakuma, Yoichi; Yamanishi, Hirokuni; Ogata, Yoshimune

    2004-01-01

    Low level liquid scintillation counting took much time with a lot of doing to distill off the impurities in the sample water before mixing the sample with the liquid scintillation cocktail. In the light of it, we investigated the possibility of an alternative filtration method for sample purification. The tritium concentration in the environmental water has become very low, and the samples have to be treated by electrolysis enrichment with a liquid scintillation analyzer. Using the solid polymer electrolyte enriching device, there is no need to add neither any electrolyte nor the neutralization after the concentration. If we could replace the distillation process with the filtration, the procedure would be simplified very much. We investigated the procedure and we were able to prove that the reverse osmosis (RO) filtration was available. Moreover, in order to rationalize all through the measurement method, we examined the followings: (1) Improvement of the enriching apparatus. (2) Easier measurement of heavy water concentration using a density meter, instead of a mass spectrometer. The concentration of water samples was measured to determine the enrichment rate of tritium during the electrolysis enrichment. (author)

  11. Measurement of the specific airway resistance by plethysmography in young children accompanied by an adult

    DEFF Research Database (Denmark)

    Klug, B; Bisgaard, H

    1997-01-01

    dependency of sRaw was investigated and the accuracy of simulating body temperature, atmospheric pressure and saturation with water vapour (BTPS) conditions by electronic compensation was assessed. One hundred and thirty one children with asthma were studied. In 57 children (mean (SD) age 5.6 (1.8) yrs) who....... In conclusion, the use of electronic compensation for simulating body temperature, atmospheric pressure and saturation with water vapour introduces a bias that affects the accuracy of the estimate of specific airway resistance. Nevertheless, plethysmographic measurements with and without an accompanying adult......The purpose of this study was to evaluate a procedure for measurement of specific airway resistance (sRaw) by whole body plethysmography in young awake children accompanied by an adult. sRaw was measured by a single-step procedure, omitting the measurement of the thoracic gas volume. The frequency...

  12. IFCC guideline for sampling, measuring and reporting ionized magnesium in plasma

    DEFF Research Database (Denmark)

    Rayana, M.C. Ben; Burnett, R.W.; Covington, A.K.

    2008-01-01

    Analyzers with ion-selective electrodes (ISEs) for ionized magnesium (iMg) should yield comparable and unbiased results for iMg. This IFCC guideline on sampling, measuring and reporting iMg in plasma provides a prerequisite to achieve this goal [in this document, "plasma" refers to circulating pl...

  13. Exact sampling of the unobserved covariates in Bayesian spline models for measurement error problems.

    Science.gov (United States)

    Bhadra, Anindya; Carroll, Raymond J

    2016-07-01

    In truncated polynomial spline or B-spline models where the covariates are measured with error, a fully Bayesian approach to model fitting requires the covariates and model parameters to be sampled at every Markov chain Monte Carlo iteration. Sampling the unobserved covariates poses a major computational problem and usually Gibbs sampling is not possible. This forces the practitioner to use a Metropolis-Hastings step which might suffer from unacceptable performance due to poor mixing and might require careful tuning. In this article we show for the cases of truncated polynomial spline or B-spline models of degree equal to one, the complete conditional distribution of the covariates measured with error is available explicitly as a mixture of double-truncated normals, thereby enabling a Gibbs sampling scheme. We demonstrate via a simulation study that our technique performs favorably in terms of computational efficiency and statistical performance. Our results indicate up to 62 and 54 % increase in mean integrated squared error efficiency when compared to existing alternatives while using truncated polynomial splines and B-splines respectively. Furthermore, there is evidence that the gain in efficiency increases with the measurement error variance, indicating the proposed method is a particularly valuable tool for challenging applications that present high measurement error. We conclude with a demonstration on a nutritional epidemiology data set from the NIH-AARP study and by pointing out some possible extensions of the current work.

  14. Self-Esteem: Assessing Measurement Equivalence in a Multiethnic Sample of Youth

    Science.gov (United States)

    Michaels, Marcia L.; Barr, Alicia; Roosa, Mark W.; Knight, George P.

    2007-01-01

    Global self-worth and five domains of self-esteem (scholastic competence, athletic competence, physical appearance, behavioral conduct, social acceptance) were tested for measurement equivalence in a sample of Anglo American, Mexican American, African American, and Native American youth aged 9 through 14 years. The results revealed that global…

  15. Bundle of Hysteresis Loops at Temperature Sweep Measured by Vibrating Sample Magnetometer

    Czech Academy of Sciences Publication Activity Database

    Vondráček, Martin; Žák, Tomáš

    2002-01-01

    Roč. 53, 10/s (2002), s. 142-144 ISSN 1335-3632. [Magnetic Measurements 2002. Bratislava, 11.09.2002-13.09.2002] R&D Projects: GA ČR GA102/01/1335 Institutional research plan: CEZ:AV0Z2041904 Keywords : vibrating sample magnetometer * hysteresis loop- thermomagnetic curve Subject RIV: BM - Solid Matter Physics ; Magnetism

  16. Method for Measuring Thermal Conductivity of Small Samples Having Very Low Thermal Conductivity

    Science.gov (United States)

    Miller, Robert A.; Kuczmarski, Maria a.

    2009-01-01

    This paper describes the development of a hot plate method capable of using air as a standard reference material for the steady-state measurement of the thermal conductivity of very small test samples having thermal conductivity on the order of air. As with other approaches, care is taken to ensure that the heat flow through the test sample is essentially one-dimensional. However, unlike other approaches, no attempt is made to use heated guards to block the flow of heat from the hot plate to the surroundings. It is argued that since large correction factors must be applied to account for guard imperfections when sample dimensions are small, it may be preferable to simply measure and correct for the heat that flows from the heater disc to directions other than into the sample. Experimental measurements taken in a prototype apparatus, combined with extensive computational modeling of the heat transfer in the apparatus, show that sufficiently accurate measurements can be obtained to allow determination of the thermal conductivity of low thermal conductivity materials. Suggestions are made for further improvements in the method based on results from regression analyses of the generated data.

  17. Sample dilution : A methodological pitfall in the measurement of tissue but not serum ACE-activity

    NARCIS (Netherlands)

    Koiter, J; Navis, G; de Jong, PE; van Gilst, WH; de Zeeuw, D

    Many tissue ACE-assays suffer from underestimation of the ACE-activity at low sample dilutions. However, measurement of ACE-activity as the amount of hippuric acid produced by cleavage of the commonly used substrate hippuryl-histidyl-leucine might circumvent this problem. In this study, we

  18. Measuring Saccade Peak Velocity Using a Low-Frequency Sampling Rate of 50 Hz

    NARCIS (Netherlands)

    Wierts, Roel; Janssen, Maurice J. A.; Kingma, Herman

    2008-01-01

    During the last decades, small head-mounted video eye trackers have been developed in order to record eye movements. Real-time systems-with a low sampling frequency of 50/60 Hz are used for clinical vestibular practice, but are generally considered not to be suited for measuring fast eye movements.

  19. The challenging measurement of protein in complex biomass-derived samples

    DEFF Research Database (Denmark)

    Haven, M.O.; Jørgensen, H.

    2014-01-01

    Measurement of the protein content in samples from production of lignocellulosic bioethanol is an important tool when studying the adsorption of cellulases. Several methods have been used for this, and after reviewing the literature, we concluded that one of the most promising assays for simple...

  20. The feasible research with measuring radon for taking the soils sample

    International Nuclear Information System (INIS)

    Zeng Bing, Ge Liangquan; Liu Hefan; Li Yeqiang; Zhang Jinzhao; Song Xiao'an

    2010-01-01

    It explains the mechanism of the separation of soil's radon. Through the designed experiment, it confirms the feasibility of measuring radon for taking the soil's sample. It determines the content of the radon and its sub field with indoor and outside through ways of the activated charcoal adsorption, the initiative suction and the diameter mark etching, also the 226 Ra. The paper indicates: it is feasible with measuring radon for taking the soil's sample, and the stability of data is that indoor data are better than outside's. The temperature, the humidity, the rainfall amount, the intensity and so on are the serious influence of the data. If you want to take a soil's sample, you must avoid the rain as far as possible, and avoid the fault zone, the belt of folded strata and complex geologic structure region, and so on. (authors)

  1. Preparedness Procedure and Environmental Radionuclides Sampling Measurement and Monitoring in Malaysia caused from Fukushima Nuclear Accident

    International Nuclear Information System (INIS)

    Nur Azna Mahmud; Zalina Laili; Muhamat Omar

    2011-01-01

    Nuclear accident in Fukushima, Japan, had caused the release of the radioactive material into the air. Our environment might be contaminated through radioactive fallout. Thus, environmental monitoring programme was activated after the accident covering areas that might be affected. Coordination of actions was made among the relevant agencies to face the emergency situation. Sample analysis was carried out by Nuklear Malaysia on environmental samples consist of rain water, sand, flora and fauna were collected and analysed using gamma spectrometer systems. This analysis was intended to measure the activity concentration of the fission products such as I-131, Cs-134 and Cs-137. Thus, a standard procedure for measurement has been developed in order to analyse the environmental samples from this emergency case. This procedure can be used as a reference in the future when such emergency situation occurs. (author)

  2. A comparison of two transient methods of measuring thermal conductivity of particulate samples.

    Science.gov (United States)

    Scott, R. W.; Fountain, J. A.; West, E. A.

    1973-01-01

    A comparison is made of the line source (LS) method and the differential line source (DLS) method of measuring thermal conductivity of particulate materials in vacuum. The DLS method requires more instrumentation in the measuring circuitry (an additional amplifier and a differentiating circuit), but since it does not require a stable temperature to initiate a test, it does not need a sample temperature control system. DLS tests can be taken as the temperature in the samples is rising from liquid nitrogen temperature to room temperature. This eliminates the practice of extrapolating thermal conductivity over this large temperature range. Also, the advantages of reduced test time, data reduction time, and small sample temperature rise enable the experimenter to take about 7-12 DLS tests in the time of 2 LS tests. Test data from the two methods agree very well.

  3. X RF measurement for sediment sample collected from the niles in Khartoum State

    International Nuclear Information System (INIS)

    Mohammed, Aisha Abdelgader Ahmed

    2015-04-01

    The goal of this study to determine the concentration of element (K, Ca Ti, Mn, Fe, Cu, Zn, Pb, Rb, Sr, Y and Zr) in sediment sample collected from thirty different sites from the three rivers in Khartoum state. X-ray fluorescence (X RF) was used to identify the element concentration. Validation of the equipment was done by measuring reference samples provided by IAEA. Iron was found to be the dominant element with concentration ranged between (13000 ppm) in the white nile to (101000 ppm) in the blue nile, Pb was found to be the lowest among the twelve elements measured with concentration ranged between (11.7 ppm) in the white nile to (28 ppm) in the river nile for all samples. In general there was no variation of the concentration of elements between the blue nile sediments and the river nile sediments and was found variation of concentration of elements between the white nile sediments and other two niles. (Author).

  4. Measurement Invariance of the WHODAS 2.0 in a Population-Based Sample of Youth.

    Directory of Open Access Journals (Sweden)

    Melissa Kimber

    Full Text Available The World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0 is a brief measure of global disability originally developed for adults, which has since been implemented among samples of children and youth. However, evidence of its validity for use among youth, particularly measurement invariance, is lacking. Investigations of measurement invariance assess the extent to which the psychometric properties of observed items in a measure are generalizable across samples. Satisfying the assumption of measurement invariance is critical for any inferences about between-group differences. The objective of this paper was to empirically assess the measurement invariance of the 12-item interview version of the WHODAS 2.0 measure in an epidemiological sample of youth (15 to 17 years and adults (≥ 18 years in Canada. Multiple-group confirmatory factor analysis using a categorical variable framework allowed for the sequential testing of increasingly restrictive models to evaluate measurement invariance of the WHODAS 2.0 between adults and youth. Findings provided evidence for full measurement invariance of the WHODAS 2.0 in youth aged 15 to 17 years. The final model fit the data well: χ(2(159 = 769.04, p < .001; CFI = 0.950, TLI = 0.958, RMSEA (90% CI = 0.055 [0.051, 0.059]. Results from this study build on previous work supporting the validity of the WHODAS 2.0. Findings indicate that the WHODAS 2.0 is valid for making substantive comparisons of disability among youth as young as 15 years of age.

  5. Preparing and measuring ultra-small radiocarbon samples with the ARTEMIS AMS facility in Saclay, France

    Energy Technology Data Exchange (ETDEWEB)

    Delque-Kolic, E., E-mail: emmanuelle.delque-kolic@cea.fr [LMC14, CEA Saclay, Batiment 450 Porte 4E, 91191 Gif sur Yvette (France); Comby-Zerbino, C.; Ferkane, S.; Moreau, C.; Dumoulin, J.P.; Caffy, I.; Souprayen, C.; Quiles, A.; Bavay, D.; Hain, S.; Setti, V. [LMC14, CEA Saclay, Batiment 450 Porte 4E, 91191 Gif sur Yvette (France)

    2013-01-15

    The ARTEMIS facility in Saclay France measures, on average, 4500 samples a year for French organizations working in an array of fields, including environmental sciences, archeology and hydrology. In response to an increasing demand for the isolation of specific soil compounds and organic water fractions, we were motivated to evaluate our ability to reduce microgram samples using our standard graphitization lines and to measure the graphite thus obtained with our 3MV NEC Pelletron AMS. Our reduction facility consists of two fully automated graphitization lines. Each line has 12 reduction reactors with a reduction volume of 18 ml for the first line and 12 ml for the second. Under routine conditions, we determined that we could reduce the samples down to 10 {mu}g of carbon, even if the graphitization yield is consequently affected by the lower sample mass. Our results when testing different Fe/C ratios suggest that an amount of 1.5 mg of Fe powder was ideal (instead of lower amounts of catalyst) to prevent the sample from deteriorating too quickly under the Cs+ beam, and to facilitate pressing procedures. Several sets of microsamples produced from HOxI standard, international references and backgrounds were measured. When measuring {sup 14}C-free wood charcoal and HOxI samples we determined that our modern and dead blanks, due to the various preparation steps, were of 1.1 {+-} 0.8 and 0.2 {+-} 0.1 {mu}g, respectively. The results presented here were obtained for IAEA-C1, {sup 14}C-free wood, IAEA-C6, IAEA-C2 and FIRI C.

  6. Study of Efficiency Calibrations of HPGe Detectors for Radioactivity Measurements of Environmental Samples

    International Nuclear Information System (INIS)

    Harb, S.; Salahel Din, K.; Abbady, A.

    2009-01-01

    In this paper, we describe a method of calibrating of efficiency of a HPGe gamma-ray spectrometry of bulk environmental samples (Tea, crops, water, and soil) is a significant part of the environmental radioactivity measurements. Here we will discuss the full energy peak efficiency (FEPE) of three HPGe detectors it as a consequence, it is essential that the efficiency is determined for each set-up employed. Besides to take full advantage at gamma-ray spectrometry, a set of efficiency at several energies which covers the wide the range in energy, the large the number of radionuclides whose concentration can be determined to measure the main natural gamma-ray emitters, the efficiency should be known at least from 46.54 keV ( 210 Pb) to 1836 keV ( 88 Y). Radioactive sources were prepared from two different standards, a first mixed standard QC Y 40 containing 210 Pb, 241 Am, 109 Cd, and Co 57 , and the second QC Y 48 containing 241 Am, 109 Cd, 57 Co, 139 Ce, 113 Sn, 85 Sr, 137 Cs, 88 Y, and 60 Co is necessary in order to calculate the activity of the different radionuclides contained in a sample. In this work, we will study the efficiency calibration as a function of different parameters as:- Energy of gamma ray from 46.54 keV ( 210 Pb) to 1836 keV ( 88 Y), three different detectors A, B, and C, geometry of containers (point source, marinelli beaker, and cylindrical bottle 1 L), height of standard soil samples in bottle 250 ml, and density of standard environmental samples. These standard environmental sample must be measured before added standard solution because we will use the same environmental samples in order to consider the self absorption especially and composition in the case of volume samples.

  7. 3D-Laser-Scanning Technique Applied to Bulk Density Measurements of Apollo Lunar Samples

    Science.gov (United States)

    Macke, R. J.; Kent, J. J.; Kiefer, W. S.; Britt, D. T.

    2015-01-01

    In order to better interpret gravimetric data from orbiters such as GRAIL and LRO to understand the subsurface composition and structure of the lunar crust, it is import to have a reliable database of the density and porosity of lunar materials. To this end, we have been surveying these physical properties in both lunar meteorites and Apollo lunar samples. To measure porosity, both grain density and bulk density are required. For bulk density, our group has historically utilized sub-mm bead immersion techniques extensively, though several factors have made this technique problematic for our work with Apollo samples. Samples allocated for measurement are often smaller than optimal for the technique, leading to large error bars. Also, for some samples we were required to use pure alumina beads instead of our usual glass beads. The alumina beads were subject to undesirable static effects, producing unreliable results. Other investigators have tested the use of 3d laser scanners on meteorites for measuring bulk volumes. Early work, though promising, was plagued with difficulties including poor response on dark or reflective surfaces, difficulty reproducing sharp edges, and large processing time for producing shape models. Due to progress in technology, however, laser scanners have improved considerably in recent years. We tested this technique on 27 lunar samples in the Apollo collection using a scanner at NASA Johnson Space Center. We found it to be reliable and more precise than beads, with the added benefit that it involves no direct contact with the sample, enabling the study of particularly friable samples for which bead immersion is not possible

  8. Reimiep 87. An interlaboratory U-235 enrichment determination by gamma measurement on solid UF6 sample

    International Nuclear Information System (INIS)

    Aparo, M.; Cresti, P.

    1988-01-01

    Gamma spectroscopy technique, based on the measurement of U 235 186 KeV flux, is now currently used for the determination of Uranium enrichment in different material of nuclear fuel cycle, namely: Uranium metallic, UO 2 pellets, UF 6 liquid or solid. The present paper describes the use of such a technique and the obtained results in determining the U 235 /U atomic isotopic abundance on a certified UF 6 solid sample. The measurements have been carried out in the frame work of the partecipation to the ''UF 6 Interlaboratory Measurements Evaluation Programme'' organized by CBNM/Geel with the support of the ESARDA (European Safeguards Research and Development Association)

  9. Selection of electrodes for the ˝in situ˝ electrical resistivity measurements of molten aluminium

    Directory of Open Access Journals (Sweden)

    Petrič M.

    2013-01-01

    Full Text Available The aim of this paper is the selections of proper electrode material for four-probe technique electrical resistivity measurement of aluminium and aluminium alloys. The biggest problem of electrodes is oxidation during measurement causing high contact resistance and giving wrong results. Various materials have been tested and aluminium electrodes have been chosen. Advantage of aluminium electrodes is that they are melted in specimen right after the pouring and causing no interface which is resulting with any contact resistance. The device together with measuring cell for ˝in situ˝ measurement of electrical resistivity was developed using four-probe DC technique.

  10. Determination of a Wear Initiation Cycle by using a Contact Resistance Measurement in Nuclear Fuel Fretting

    International Nuclear Information System (INIS)

    Lee, Young Ho; Kim, Hyung Kyu

    2008-01-01

    In nuclear fuel fretting, the improving of the contact condition with a modified spring shape is a useful method for increasing the wear resistance of the nuclear fuel rod. This is because the fretting wear resistance between the fuel rod and grid spring is mainly affected by the grid spring shape rather than the environment, the contact modes, etc. In addition, the wear resistance is affected by the wear debris behavior between contact surfaces. So, it is expected that the wear initiation of each spring shape should be determined in order to evaluate a wear resistance. However, it is almost impossible to measure the wear behavior in contact surfaces on a real time basis because the contact surfaces are always hidden. Besides, the results of the worn surface observation after the fretting wear tests are restricted to archive the information on the wear debris behavior and the formation mechanism of the wear scar. In order to evaluate the wear behavior during the fretting wear tests, it is proposed that the contact resistance measurement is a useful method for examining the wear initiation cycle and modes. Generally, fretting wear damages are rapidly progressed by a localized plastic deformation between the contact surfaces, crack initiation and fracture of the deformed surface with a strain hardening difference between a surface and a subsurface and finally a detachment of wear debris. After this, wear debris is easily oxidized by frictional heat, test environment, etc. At this time, a small amount of electric current applied between the contact surfaces will be influenced by the wear debris, which could be an obstacle to an electric current flow. So, it is possible to archive the information on the wear behavior by measuring the contact resistance. In order to determine the wear initiation cycle during the fretting wear tests, in this study, fretting wear tests have been performed by applying a constant electric current in room temperature air

  11. Strategic measures for the control of surging antimicrobial resistance in Hong Kong and mainland of China.

    Science.gov (United States)

    Cheng, Vincent C C; Wong, Sally C Y; Ho, Pak-Leung; Yuen, Kwok-Yung

    2015-02-01

    Antimicrobial-resistant bacteria are either highly prevalent or increasing rapidly in Hong Kong and China. Treatment options for these bacteria are generally limited, less effective and more expensive. The emergence and dynamics of antimicrobial resistance genes in bacteria circulating between animals, the environment and humans are not entirely known. Nonetheless, selective pressure by antibiotics on the microbiomes of animal and human, and their associated environments (especially farms and healthcare institutions), sewage systems and soil are likely to confer survival advantages upon bacteria with antimicrobial-resistance genes, which may be further disseminated through plasmids or transposons with integrons. Therefore, antibiotic use must be tightly regulated to eliminate such selective pressure, including the illegalization of antibiotics as growth promoters in animal feed and regulation of antibiotic use in veterinary practice and human medicine. Heightened awareness of infection control measures to reduce the risk of acquiring resistant bacteria is essential, especially during antimicrobial use or institutionalization in healthcare facilities. The transmission cycle must be interrupted by proper hand hygiene, environmental cleaning, avoidance of undercooked or raw food and compliance with infection control measures by healthcare workers, visitors and patients, especially during treatment with antibiotics. In addition to these routine measures, proactive microbiological screening of hospitalized patients with risk factors for carrying resistant bacteria, including history of travel to endemic countries, transfer from other hospitals, and prolonged hospitalization; directly observed hand hygiene before oral intake of drugs, food and drinks; and targeted disinfection of high-touch or mutual-touch items, such as bed rails and bed curtains, are important. Transparency of surveillance data from each institute for public scrutiny provides an incentive for

  12. Effectiveness of simple control measures on methicillin-resistant Staphylococcus aureus infection status and characteristics with susceptibility patterns in a teaching hospital in Peshawar.

    Science.gov (United States)

    Rafiq, Muhammad Salman; Rafiq, Muhammad Imran; Khan, Taimur; Rafiq, Maria; Khan, Mah Muneer

    2015-09-01

    To determine the effectiveness of simple control measures on the infection status and characteristics of methicillin-resistant Staphylococcus aureus including susceptibility patterns among health professionals and patients in a teaching hospital. The cross-sectional study was conducted from September 2013 to January 2014, and comprised samples collected from healthcare personnel and patients in the various units of Khyber Teaching Hospital, Peshawar. The specimens were collected before and one month after the implementation of simple control measures for outbreak prevention of methicillin-resistant Staphylococcus aureus. These were tested for culture and antimicrobial susceptibility. Data about methicillin-sensitive and methicillin-resistant Staphylococcus aureus infection, wound characteristics and susceptibility patterns was collected and effectiveness of simple control measures was determined. SPSS 20 was used for statistical analysis. Of the total 390 isolates, 180(46.2%) were Staphylococcus aureus; 77(19.7%) from healthcare personnel and 103(26.4%) from patients. Of these, 164(42.1%) were methicillin-sensitive and 16(4.1%) were methicillin-resistant. Among the patients, 38(15.1%) methicillin-sensitive and 8(3.2%) methicillin-resistant isolates were recovered from wounds or skin and soft tissues. Pus with 33(13.1%) and 4(1.6%) cases respectively was the second most common source. Among methicillin-resistant isolates, resistance to Linezolid was 0%, all were resistant to Oxacillin, Cefoxitin, Amoxicillin, Cefotaxime and Cephradine, and resistance to both Co-Amoxiclav and Ciprofloxacin was 87.5%. After one month of implementation of simple control measures, the number of methicillin-resistant cases among healthcare professionals and patients dropped from 4(2.9%) and 7(10.8%) to 1(0.7%) and 5(2.7%), respectively. Methicillin-resistant and methicillin-sensitive Staphylococcus aureus differed in their anti-microbial susceptibility profiles. Selection of antibiotics

  13. New approach of a transient ICP-MS measurement method for samples with high salinity.

    Science.gov (United States)

    Hein, Christina; Sander, Jonas Michael; Kautenburger, Ralf

    2017-03-01

    In the near future it is necessary to establish a disposal for high level nuclear waste (HLW) in deep and stable geological formations. In Germany typical host rocks are salt or claystone. Suitable clay formations exist in the south and in the north of Germany. The geochemical conditions of these clay formations show a strong difference. In the northern ionic strengths of the pore water up to 5M are observed. The determination of parameters like K d values during sorption experiments of metal ions like uranium or europium as homologues for trivalent actinides onto clay stones are very important for long term safety analysis. The measurement of the low concentrated, not sorbed analytes commonly takes place by inductively coupled plasma mass spectrometry (ICP-MS). A direct measurement of high saline samples like seawater with more than 1% total dissolved salt content is not possible. Alternatives like sample clean up, preconcentration or strong dilution have more disadvantages than advantages for example more preparation steps or additional and expensive components. With a small modification of the ICP-MS sample introduction system and a home-made reprogramming of the autosampler a transient analysing method was developed which is suitable for measuring metal ions like europium and uranium in high saline sample matrices up to 5M (NaCl). Comparisons at low ionic strength between the default and the transient measurement show the latter performs similarly well to the default measurement. Additionally no time consuming sample clean-up or expensive online dilution or matrix removal systems are necessary and the analysation shows a high sensitivity due to the data processing based on the peak area. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Antibiotic Resistance in Animal and Environmental Samples Associated with Small-Scale Poultry Farming in Northwestern Ecuador.

    Science.gov (United States)

    Braykov, Nikolay P; Eisenberg, Joseph N S; Grossman, Marissa; Zhang, Lixin; Vasco, Karla; Cevallos, William; Muñoz, Diana; Acevedo, Andrés; Moser, Kara A; Marrs, Carl F; Foxman, Betsy; Trostle, James; Trueba, Gabriel; Levy, Karen

    2016-01-01

    The effects of animal agriculture on the spread of antibiotic resistance (AR) are cross-cutting and thus require a multidisciplinary perspective. Here we use ecological, epidemiological, and ethnographic methods to examine populations of Escherichia coli circulating in the production poultry farming environment versus the domestic environment in rural Ecuador, where small-scale poultry production employing nontherapeutic antibiotics is increasingly common. We sampled 262 "production birds" (commercially raised broiler chickens and laying hens) and 455 "household birds" (raised for domestic use) and household and coop environmental samples from 17 villages between 2010 and 2013. We analyzed data on zones of inhibition from Kirby-Bauer tests, rather than established clinical breakpoints for AR, to distinguish between populations of organisms. We saw significantly higher levels of AR in bacteria from production versus household birds; resistance to either amoxicillin-clavulanate, cephalothin, cefotaxime, and gentamicin was found in 52.8% of production bird isolates and 16% of household ones. A strain jointly resistant to the 4 drugs was exclusive to a subset of isolates from production birds (7.6%) and coop surfaces (6.5%) and was associated with a particular purchase site. The prevalence of AR in production birds declined with bird age (P < 0.01 for all antibiotics tested except tetracycline, sulfisoxazole, and trimethoprim-sulfamethoxazole). Farming status did not impact AR in domestic environments at the household or village level. Our results suggest that AR associated with small-scale poultry farming is present in the immediate production environment and likely originates from sources outside the study area. These outside sources might be a better place to target control efforts than local management practices. IMPORTANCE In developing countries, small-scale poultry farming employing antibiotics as growth promoters is being advanced as an inexpensive source of

  15. Measurement of I-129 in environmental samples by ICP-CRI-QMS. Possibilities and limitations

    Energy Technology Data Exchange (ETDEWEB)

    Li, K.; Vogel, E.; Kraehenbuehl, U. [Dept. of Chemistry and Biochemistry, Univ. of Bern (Switzerland)

    2009-07-01

    {sup 129}I is preconcentrated from environmental samples and its accessibility is addressed for inductively coupled plasma quadrupole mass spectrometer with collision/reaction interface (ICP-CRI-QMS). By applying oxygen as CRI gas through skimmer cone, the signal of the interfering {sup 129}Xe from the impurity of plasma gas can be eliminated while the formation of {sup 127}I{sup 1}H{sup 1}H{sup +} can be partially removed. The improved ICP-QMS can be employed for investigation of {sup 129}I in environmental samples with a {sup 129}I/{sup 127}I ratio down to 10{sup -7}. The detection capability was demonstrated by measuring {sup 129}I in seaweed samples collected around the nuclear fuel reprocessing plant at La Hague. The abundant {sup 127}I in the sample matrix causes a serious problem and degrades the detection capability of the instrument when the concentration of {sup 127}I is larger than 10{sup 5} ng/g. Even combined with a sufficient pre-concentration procedure, ICP-QMS is not considered as a suitable technique for the analysis of {sup 129}I in uncontaminated environmental samples with {sup 129}I/{sup 127}I ratio of less than 10{sup -7}. Results are presented for seaweed samples collected around La Hague. In addition, time dependant I concentrations and ratios are given for colloids and water sampled from Lake Thun. (orig.)

  16. Multi-Sampling Ionization Chamber (MUSIC) for measurements of fusion reactions with radioactive beams

    International Nuclear Information System (INIS)

    Carnelli, P.F.F.; Almaraz-Calderon, S.; Rehm, K.E.; Albers, M.; Alcorta, M.; Bertone, P.F.; Digiovine, B.; Esbensen, H.; Fernández Niello, J.; Henderson, D.; Jiang, C.L.; Lai, J.; Marley, S.T.; Nusair, O.; Palchan-Hazan, T.; Pardo, R.C.; Paul, M.; Ugalde, C.

    2015-01-01

    A detection technique for high-efficiency measurements of fusion reactions with low-intensity radioactive beams was developed. The technique is based on a Multi-Sampling Ionization Chamber (MUSIC) operating as an active target and detection system, where the ionization gas acts as both target and counting gas. In this way, we can sample an excitation function in an energy range determined by the gas pressure, without changing the beam energy. The detector provides internal normalization to the incident beam and drastically reduces the measuring time. In a first experiment we tested the performance of the technique by measuring the 10,13,15 C+ 12 C fusion reactions at energies around the Coulomb barrier

  17. Multi-Sampling Ionization Chamber (MUSIC) for measurements of fusion reactions with radioactive beams

    Energy Technology Data Exchange (ETDEWEB)

    Carnelli, P.F.F. [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Laboratorio TANDAR, Comisión Nacional de Energía Atómica, Av. Gral. Paz 1499, B1650KNA, San Martín, Buenos Aires (Argentina); Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917, C1033AAJ Buenos Aires (Argentina); Almaraz-Calderon, S. [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Rehm, K.E., E-mail: rehm@anl.gov [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Albers, M.; Alcorta, M.; Bertone, P.F.; Digiovine, B.; Esbensen, H. [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Fernández Niello, J. [Laboratorio TANDAR, Comisión Nacional de Energía Atómica, Av. Gral. Paz 1499, B1650KNA, San Martín, Buenos Aires (Argentina); Universidad Nacional de San Martín, Campus Miguelete, B1650BWA San Martín, Buenos Aires (Argentina); Henderson, D.; Jiang, C.L. [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Lai, J. [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, LA 70803 (United States); Marley, S.T.; Nusair, O.; Palchan-Hazan, T.; Pardo, R.C. [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Paul, M. [Racah Institute of Physics, Hebrew University, Jerusalem (Israel); Ugalde, C. [Physics Division, Argonne National Laboratory, Argonne, IL 60439 (United States)

    2015-11-01

    A detection technique for high-efficiency measurements of fusion reactions with low-intensity radioactive beams was developed. The technique is based on a Multi-Sampling Ionization Chamber (MUSIC) operating as an active target and detection system, where the ionization gas acts as both target and counting gas. In this way, we can sample an excitation function in an energy range determined by the gas pressure, without changing the beam energy. The detector provides internal normalization to the incident beam and drastically reduces the measuring time. In a first experiment we tested the performance of the technique by measuring the {sup 10,13,15}C+{sup 12}C fusion reactions at energies around the Coulomb barrier.

  18. Specific calibration problems for gammaspectrometric measurements of low-level radioactivity in environmental samples

    Energy Technology Data Exchange (ETDEWEB)

    Arnold, D. [Physikalisch-Technische Bundesanstalt, Braunschweig (Germany); Wershofen, H. [Physikalisch-Technische Bundesanstalt, Braunschweig (Germany)

    1997-03-01

    Gammaspectrometric measurements of low-level radioactivity in environmental samples are always done in a close source detector geometry. This geometry causes coincidence-summing effects for measurements of multi-photon emitting nuclides. The measurements of radioactivity in environmental samples are also influenced by the absorption of photons in the materials which have to be analysed. Both effects must be taken into account by correction factors with respect to an energy-specific calibration of the detector system for a given geometry and a given composition of the calibration source. The importance of these corrections is emphasized. It is the aim of the present paper to compare different experimental and theoretical methods for the determination of these correction factors published by various authors and to report about efforts to refine them. (orig.)

  19. The impact of finite-area inhomogeneities on resistive and Hall measurement

    Science.gov (United States)

    Koon, Daniel

    2013-03-01

    I derive an iterative expression for the electric potential in an otherwise homogeneous thin specimen as the result of a finite-area inhomogeneity in either the direct conductance, the Hall conductance, or both. This expression extends to the finite-area regime the calculation of the effect of such inhomogeneities on the measurement error in the sheet resistance and Hall sheet resistance. I then test these results on the exactly-solvable case of a circular inhomogeneity equally distant from the four electrodes of either a square four-point-probe array on an infinitely large conducting specimen or a circular van der Pauw specimen with symmetrically-placed electrodes.

  20. Antibiotic Exposure in a Low-Income Country: Screening Urine Samples for Presence of Antibiotics and Antibiotic Resistance in Coagulase Negative Staphylococcal Contaminants

    DEFF Research Database (Denmark)

    Lerbeck, Anne Mette; Tersbøl, Britt Pinkowski; Styrishave, Bjarne

    2014-01-01

    Development of antimicrobial resistance has been assigned to excess and misuse of antimicrobial agents. Staphylococci are part of the normal flora but are also potential pathogens that have become essentially resistant to many known antibiotics. Resistances in coagulase negative staphylococci (Co......NS) are suggested to evolve due to positive selective pressure following antibiotic treatment. This study investigated the presence of the nine most commonly used antimicrobial agents in human urine from outpatients in two hospitals in Ghana in relation to CoNS resistance. Urine and CoNS were sampled (n5246 and n...... was the most common isolate (75%), followed by S. epidermidis (13%) and S. hominis (6%). S. haemolyticus was also the species displaying the highest resistance prevalence (82%). 69% of the isolated CoNS were multiple drug resistant (§4 antibiotics) and 45% of the CoNS were methicillin resistant. Antimicrobial...

  1. Dermabacter hominis: a usually daptomycin-resistant gram-positive organism infrequently isolated from human clinical samples

    Science.gov (United States)

    Fernández-Natal, I; Sáez-Nieto, J A; Medina-Pascual, M J; Albersmeier, A; Valdezate, S; Guerra-Laso, J M; Rodríguez, H; Marrodán, T; Parras, T; Tauch, A; Soriano, F

    2013-01-01

    During a 12-year period, Dermabacter hominis was isolated from 21 clinical samples belonging to 14 patients attending a tertiary hospital in León, Spain. Samples included blood cultures (14), peritoneal dialysis catheter exit sites (three), cutaneous abscesses (two), an infected vascular catheter (one) and a wound swab (one). Identification was made by API Coryne™ V2.0, Biolog™ GP2 and 16S rRNA gene amplification. Six febrile patients had positive blood cultures (one, two or three sets) and all of them were treated with teicoplanin (two patients), vancomycin, ampicillin plus gentamicin, amoxicillin/clavulanic acid and ciprofloxacin (one each). An additional patient with a single positive blood culture was not treated, the finding being considered non-significant. In the remaining seven patients the organism was isolated from a single specimen and three of them received antimicrobial treatment (ciprofloxacin, ceftriaxone plus vancomycin and amoxicillin/clavulanic acid). At least ten patients had several underlying diseases and conditions, and no direct mortality was observed in relation to the isolated organism. All isolates were susceptible to vancomycin, rifampin and linezolid. Resistance to other antibiotics varied: erythromycin (100%), clindamycin (78.5%), ciprofloxacin (21.4%) and gentamicin, quinupristin-dalfopristin, benzylpenicillin and imipenem 7.1% each. Thirteen isolates were highly resistant to daptomycin with MICs ranging from 8 to 48 (MIC90 = 32 mg/L); only one was daptomycin-sensitive (MIC = 0.19 mg/L). PMID:25356327

  2. Acyclovir-resistant herpes simplex virus type 1 in intra-ocular fluid samples of herpetic uveitis patients.

    Science.gov (United States)

    van Velzen, Monique; Missotten, Tom; van Loenen, Freek B; Meesters, Roland J W; Luider, Theo M; Baarsma, G Seerp; Osterhaus, Albert D M E; Verjans, Georges M G M

    2013-07-01

    Acyclovir (ACV) is the antiviral drug of choice to treat patients with herpes simplex virus type 1 (HSV-1) uveitis. The prevalence of intra-ocular ACV-resistant (ACV(R)) HSV-1 in herpetic uveitis is unknown and may have clinical consequences. In addition to its predictive value on ACV susceptibility, the polymorphic HSV-1 thymidine kinase (TK) gene facilitates differentiation between HSV-1 strains. The objective of this study was to determine the genetic composition and ACV susceptibility of the causative virus in intra-ocular fluid samples (IOF) of HSV-1 uveitis patients. The intra-ocular HSV-1 pool from 11 HSV-1 uveitis patients was determined by sequencing IOF-derived viral TK genes. The ACV susceptibility profile of the cloned intra-ocular TK variants was defined by mass spectrometry. In addition, the ganciclovir (GCV) susceptibility of the ACV(R) HSV-1 TK variants was defined. Intra-ocular fluid samples of HSV-1 uveitis patients contain HSV-1 quasispecies, principally consisting of one major and multiple genetically related minor patient-specific TK variants. Four of 10 patients analyzed had an intra-ocular ACV(R) HSV-1 of which 3 were cross-resistant to GCV. The ACV(R) profile of intra-ocular HSV-1 did not correlate with symptomatic ACV treatment. Affected eyes of HSV-1 uveitis patients are commonly infected with a patient-specific HSV-1 quasispecies, including one major and multiple genetically related minor variants. A relatively high prevalence of intra-ocular ACV(R) HSV-1, mainly ACV/GCV cross-resistant viruses, was detected in HSV-1 uveitis patients. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Quantitative Digital Autoradiography for Environmental Swipe Sample Prioritization: System design, Characterization, and Initial Measurements

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, Benjamin S.; Zalavadia, Mital A.; Miller, Brian W.; Bliss, Mary; Olsen, Khris B.; Kasparek, Dustin M.; Clarke, Ardelia M.

    2017-07-17

    Environmental sampling and sample analyses by the International Atomic Energy Agency’s (IAEA) Network of Analytical Laboratories (NWAL) is a critical technical tool used to detect facility misuse under a Comprehensive Safeguards Agreement and to verify the absence of undeclared nuclear material activities under an Additional Protocol. Currently all environmental swipe samples (ESS) are screened using gamma spectrometry and x-ray fluorescence to estimate the amount of U and/or Pu in the ESS, to guide further analysis, and to assist in the shipment of ESS to the NWAL. Quantitative Digital Autoradiography for Environmental Samples (QDARES) is being developed to complement existing techniques through the use of a portable, real-time, high-spatial-resolution camera called the Ionizing-radiation Quantum Imaging Detector (iQID). The iQID constructs a spatial map of radionuclides within a sample or surface in real-time as charged particles (betas) and photons (gamma/x-rays) are detected and localized on an event-by-event basis. Knowledge of the location and nature of radioactive hot spots on the ESS could provide information for subsequent laboratory analysis. As a nondestructive technique, QDARES does not compromise the ESS chain of custody or subsequent laboratory analysis. In this paper we will present the system design and construction, characterization measurements with calibration sources, and initial measurements of ESS.

  4. Personality in general and clinical samples: Measurement invariance of the Multidimensional Personality Questionnaire.

    Science.gov (United States)

    Eigenhuis, Annemarie; Kamphuis, Jan H; Noordhof, Arjen

    2017-09-01

    A growing body of research suggests that the same general dimensions can describe normal and pathological personality, but most of the supporting evidence is exploratory. We aim to determine in a confirmatory framework the extent to which responses on the Multidimensional Personality Questionnaire (MPQ) are identical across general and clinical samples. We tested the Dutch brief form of the MPQ (MPQ-BF-NL) for measurement invariance across a general population subsample (N = 365) and a clinical sample (N = 365), using Multiple Group Confirmatory Factor Analysis (MGCFA) and Multiple Group Exploratory Structural Equation Modeling (MGESEM). As an omnibus personality test, the MPQ-BF-NL revealed strict invariance, indicating absence of bias. Unidimensional per scale tests for measurement invariance revealed that 10% of items appeared to contain bias across samples. Item bias only affected the scale interpretation of Achievement, with individuals from the clinical sample more readily admitting to put high demands on themselves than individuals from the general sample, regardless of trait level. This formal test of equivalence provides strong evidence for the common structure of normal and pathological personality and lends further support to the clinical utility of the MPQ. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  5. Stability and reliability of glycated haemoglobin measurements in blood samples stored at -20°C.

    Science.gov (United States)

    Venkataraman, Vijayachandrika; Anjana, Ranjit Mohan; Pradeepa, Rajendra; Deepa, Mohan; Jayashri, Ramamoorthy; Anbalagan, Viknesh Prabu; Akila, Bridgitte; Madhu, Sri Venkata; Lakshmy, Ramakrishnan; Mohan, Viswanathan

    2016-01-01

    To validate the stability of glycated haemoglobin (HbA1c) measurements in blood samples stored at -20°C for up to one month. The study group comprised 142 type 2 diabetic subjects visiting a tertiary centre for diabetes at Chennai city in south India. The HbA1c assay was done on a fasting blood sample using the Bio-Rad Variant machine on Day 0 (day of blood sample collection). Several aliquots were stored at -20°C and the assay was repeated on the 3rd, 7th, 15th, and 30th day after the sample collection. Bland-Altman plots were constructed and variation in the HbA1c levels on the different days was compared with the day 0 level. The median differences between HbA1c levels measured on Day 0 and the 3rd, 7th, 15th, and 30th day after blood collection were 0.0%, 0.2%, 0.3% and 0.5% respectively. Bland-Altman plot analysis showed that the differences between the day '0' and the different time points tend to get larger with time, but these were not clinically significant. HbA1c levels are relatively stable up to 2weeks, if blood samples are stored at -20°C. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Testing an ionization chamber with gaseous samples and measurements of the (n, alpha) reaction cross sections

    CERN Document Server

    Gledenov, Yu M; Salatskii, V I; Sedyshev, P V; Andrzejewski, J; Szalanski, P

    1999-01-01

    A new ionization chamber with gaseous samples (GIC) has been designed and tested on the thermal and resonance neutron beams of FLNP's neutron sources. The exposed gas volume serves as a target for neutrons. The obtained thermal cross sections for the sup 1 sup 7 O(n, alpha) sup 1 sup 4 C, sup 2 sup 1 Ne(n, alpha) sup 1 sup 8 O and sup 3 sup 6 Ar(n, alpha) sup 3 sup 3 S reactions are (233+-12) mb, (0.18+-0.09) mb and (5.43+-0.27) mb, respectively. These measurements have been performed on a pure beam of thermal neutrons from the high flux reactor IBR-2; and they demonstrated high efficiency and reliability of the method. Compared to samples on substrates, the application of gaseous samples makes the beam background essentially lower, and what is more important, the background component is totally absent due to the absence of Li and B microimpurities in gaseous samples while they do present in the samples on substrates. The method is also applicable to measurements with resonance neutrons. The recovery capabili...

  7. Surrogate Measures of Insulin Resistance in Middle-aged Non-diabetic Subjects

    Directory of Open Access Journals (Sweden)

    Katalin Csép

    2013-12-01

    Full Text Available Objective: Insulin resistance has been shown to be a risk factor for type 2 diabetes and cardiovascular disease. The assessment of insulin sensitivity in the clinical practice, however, faces several difficulties. The study proposes to analyze surrogate measures of insulin resistance based on fasting insulin levels in central Romania, and check whether the diagnosis of the metabolic syndrome is an adequate strategy to identify middle-aged persons with reduced insulin sensitivity. Methods: Anthropometric measurements, metabolic profile, and surrogates measures of insulin sensitivity (GIR, HOMA, QUICKI, FIRI, Belfiore, Bennett, Raynaud, McAuley index based on fasting insulin levels were assessed in 233 non-diabetic middle aged subjects. Results: Cutoff values, determined as the lowest quartile of insulin sensitivity for fasting insulin, HOMA, IRI (1/QUICKI, FIRI and Belfiore's, Bennett's, Raynaud's and McAuley's insulin sensitivity indices were 10.49 mU/L, 2.1, 3.01, 2.32, and 0.03, 1.34, 3.81, 6.29, 5.82. Components of the metabolic syndrome showed moderate but significant correlations with the surrogate measures of insulin resistance (r = 0.22-0.56, p <0.05. HOMA-IR and McAuley indices were the best predictors of clustered cardiometabolic risk factors (AUC - 0.83, 0.81 and 0.82. The metabolic syndrome diagnosis performed well in identifying patients with reduced insulin sensitivity (McAuley 2: sensitivity - 0.78, specificity - 0.84. Conclusion: Fasting insulin derived insulin sensitivity indices may help the recognittion of insulin resistant states predicting cardiometabolic disorders. Actively looking for insulin resistance by these simple indices, or by diagnosing the metabolic syndrome, those at increased risk can be recognized

  8. Implementation of an Enhanced Measurement Control Program for handling nuclear safety samples at WSRC

    International Nuclear Information System (INIS)

    Boler-Melton, C.; Holland, M.K.

    1991-01-01

    In the separation and purification of nuclear material, nuclear criticality safety (NCS) is of primary concern. The primary nuclear criticality safety controls utilized by the Savannah River Site (SRS) Separations Facilities involve administrative and process equipment controls. Additional assurance of NCS is obtained by identifying key process hold points where sampling is used to independently verify the effectiveness of production control. Nuclear safety measurements of samples from these key process locations provide a high degree of assurance that processing conditions are within administrative and procedural nuclear safety controls. An enhanced procedure management system aimed at making improvements in the quality, safety, and conduct of operation was implemented for Nuclear Safety Sample (NSS) receipt, analysis, and reporting. All procedures with nuclear safety implications were reviewed for accuracy and adequate detail to perform the analytical measurements safely, efficiently, and with the utmost quality. Laboratory personnel worked in a ''Deliberate Operating'' mode (a systematic process requiring continuous expert oversight during all phases of training, testing, and implementation) to initiate the upgrades. Thus, the effort to revise and review nuclear safety sample procedures involved a team comprised of a supervisor, chemist, and two technicians for each procedure. Each NSS procedure was upgraded to a ''Use Every Time'' (UET) procedure with sign-off steps to ensure compliance with each step for every nuclear safety sample analyzed. The upgrade program met and exceeded both the long and short term customer needs by improving measurement reliability, providing objective evidence of rigid adherence to program principles and requirements, and enhancing the system for independent verification of representative sampling from designated NCS points

  9. Methods and apparatus for measurement of the resistivity of geological formations from within cased boreholes

    Science.gov (United States)

    Vail, III, William B.

    1989-01-01

    Methods and apparatus are disclosed which allow measurement of the resistivity of a geological formation through borehole casing which may be surrounded by brine saturated cement. A.C. current is passed from an electrode in electrical contact with the interior of the borehole casing to an electrode on the surface of the earth. The A.C. voltage difference is measured between two additional vertically disposed electrodes on the interior of the casing which provides a measure of the resistivity of the geological formation. A calibration and nulling procedure is presented which minimizes the influence of variations in the thickness of the casing. The procedure also minimizes the influence of inaccurate placements of the additional vertically disposed electrodes.

  10. Magnetic induction spectroscopy: non-contact measurement of the electrical conductivity spectra of biological samples

    International Nuclear Information System (INIS)

    Barai, A; Watson, S; Patz, R; Griffiths, H

    2012-01-01

    Measurement of the electrical conductivity of biological tissues as a function of frequency, often termed ‘bioelectrical impedance spectroscopy (BIS)’, provides valuable information on tissue structure and composition. In implementing BIS though, there can be significant practical difficulties arising from the electrode–sample interface which have likely limited its deployment in industrial applications. In magnetic induction spectroscopy (MIS) these difficulties are eliminated through the use of fully non-contacting inductive coupling between the sensors and sample. However, inductive coupling introduces its own set of technical difficulties, primarily related to the small magnitudes of the induced currents and their proportionality with frequency. This paper describes the design of a practical MIS system incorporating new, highly-phase-stable electronics and compares its performance with that of electrode-based BIS in measurements on biological samples including yeast suspensions in saline (concentration 50–400 g l −1 ) and solid samples of potato, cucumber, tomato, banana and porcine liver. The shapes of the MIS spectra were in good agreement with those for electrode-based BIS, with a residual maximum discrepancy of 28%. The measurement precision of the MIS was 0.05 S m −1 at 200 kHz, improving to 0.01 S m −1 at a frequency of 20 MHz, for a sample volume of 80 ml. The data-acquisition time for each MIS measurement was 52 s. Given the value of spectroscopic conductivity information and the many advantages of obtaining these data in a non-contacting manner, even through electrically-insulating packaging materials if necessary, it is concluded that MIS is a technique with considerable potential for monitoring bio-industrial processes and product quality. (paper)

  11. A fast radiochemical procedure to measure Tc-99 in environmental samples and gaseous and liquid effluents

    International Nuclear Information System (INIS)

    Luxenburger, H.J.; Schuettelkopf, H.; Bohn, B.

    1984-08-01

    Liquid effluent samples are collected in polyethylene bottles. Sampling in the gaseous effluents relies on conventional NaOH scrubbers. No noticeable adsorption on the vessel walls takes place in HNO 3 /H 2 O 2 and NaOH, respectively, in the plastic botteles used. A known amount of Tc-95m is added in order to determine the chemical yield prior to the analysis. The pertechnetate in the samples is first fixed on an anionexchanger. Following elution with perchlorate/sulfite solution TC is coprecipitated with iron(II) hydroxide. Having dissolved the precipitation in H 2 O 2 /H 2 SO 4 one extracts with TBP/alkane. To achieve a better decontamination from other nuclides a re-extraction is performed with NaOH and after acidification with H 2 SO 4 a further extraction using TBP/Alkane. Before the measurement of the sample the alkane is eliminated in a rotation evaporator and the remaining TBP is dissolved directly in the liquid scintillator. Other beta emitters generated as fission products in the course of nuclear fission are satisfactorily separated during the analysis. The decontamination factors for I, Se, Te, Sb, and Ru are > 10 5 . The measurements are carried out with a Beckman LS 7800 instrument. The optimum channel setting is found from the spectra of the two nuclides Tc-95m and Tc-99. The quenching plots are determined accordingly. The measurements are evaluated with a table computer. Chemical yields of 50-90% are achieved. The detection limit for 600 minutes of measuring time is 1 pCi per sample. One person is capable of performing about 15 analyses per week using the method described. (orig./HP) [de

  12. The nonlinear effect of resistive inhomogeneities on van der Pauw measurements

    Science.gov (United States)

    Koon, Daniel W.

    2005-03-01

    The resistive weighting function [D. W. Koon and C. J. Knickerbocker, Rev. Sci. Instrum. 63, 207 (1992)] quantifies the effect of small local inhomogeneities on van der Pauw resistivity measurements, but assumes such effects to be linear. This talk will describe deviations from linearity for a square van der Pauw geometry, modeled using a 5 x 5 grid network of discrete resistors and introducing both positive and negative perturbations to local resistors, covering nearly two orders of magnitude in -δρ/ρ or -δσ/σ. While there is a relatively modest quadratic nonlinearity for inhomogeneities of decreasing conductivity, the nonlinear term for inhomogeneities of decreasing resistivity is approximately cubic and can exceed the linear term.

  13. Predicting a single HIV drug resistance measure from three international interpretation gold standards.

    Science.gov (United States)

    Yashik, Singh; Maurice, Mars

    2012-07-01

    To investigate the possibility of combining the interpretation of three gold standard interpretation algorithms using weighted heuristics in order to produce a single resistance measure. The outputs of HIVdb, Rega, ANRS were combined to obtain a single resistance profile using the equally weighted voting algorithm, accuracy based weighing voting algorithm and the Bayesian based weighted voting algorithm techniques. The Bayesian based voting combination increased the accuracy of the resistance profile prediction compared to phenotype, from 58% to 69%. The equal weighted voting algorithm and the accuracy based algorithm both increased the prediction accuracy to 60%. From the result obtained it is evident that combining the gold standard interpretation algorithms may increase the predictive ability of the individual interpretation algorithms. Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  14. Fatigue damage measurement based on electrical resistance change in carbon steel C45

    International Nuclear Information System (INIS)

    Atanazio Filho, Nelson N.; Mansur, Tanius Rodrigues; Gomes, Paulo T. Vida; Nogueira, Andre L.

    2009-01-01

    Structural components normally experience fatigue cycling during operation. Failure of these components is mostly due to fatigue. So, it is important to know the fatigue damage behavior and fatigue life of the material before selecting these alloys for making different structural components. Damage is a very important concept in fatigue studies. In the context of the continuous damage mechanics of materials is defined as a property that decreases the component strength, until failure. The process of damage creation consists in the superficial discontinuity nucleation (micro cracks) and/or volumetric discontinuity nucleation (micro cracks or micro-cavities) in the material. It begins where the localized stress exceeds the material yielding stress under stress or strain fluctuation. Generally mechanical components suffer damages along their entire life, since the external loads exceed the allowable stress. In applications where the fatigue problems are determinative, the damage measurement has a fundamental importance. In this case, always that the external alternate stress for superior to the fatigue strength limit, will occur an irrecoverable and cumulative damage in the material. The damage quantitative evaluation is a complex task, since it involves macroscopic and microscopic characteristics of the material. Electrical resistance measurement method has been used in terms of monitoring and damage measurement. The electrical resistance method is particularly effective for detecting small and subtle defects in structural materials and in joints. The objective of this work is measure damages caused by reverse fatigue in carbon steel C45 using electrical resistance measurement method. (author)

  15. Is Declining malaria vector population in Africa a result of intervention Measures or sampling tools inefficiency?

    Directory of Open Access Journals (Sweden)

    Eliningaya Kweka

    2013-01-01

    Full Text Available Recent entomological surveys have shown a declining trend of malaria vector population in sub-Saharan Africa and the observation have beenassociated with the scale-up and intensive use of malaria intervention measures such as insecticides treated nets and insecticide residual sprays.However, little is known on the contribution of the mosquito sampling tools inefficiency on the declining trends of malaria vector population. Inthis commentary paper, we explore the possibility of contribution of mosquito sampling tools’ inefficiency to the observed declining trends ofmalaria vector population in Africa.

  16. Sampling protocol recommendations for measuring soil organic carbon stocks in the tropics

    Science.gov (United States)

    van Straaten, Oliver; Veldkamp, Edzo; Corre, Marife D.

    2013-04-01

    In the tropics, there is an urgent need for cost effective sampling approaches to quantify soil organic carbon (SOC) changes associated with land-use change given the lack of reliable data. The tropics are especially important considering the high deforestation rates, the huge belowground carbon pool and the fast soil carbon turnover rates. In the framework of a pan-tropic (Peru, Cameroon and Indonesia) land-use change study, some highly relevant recommendations on the SOC stocks sampling approaches have emerged. In this study, where we focused on deeply weathered mineral soils, we quantified changes in SOC stock following land-use change (deforestation and subsequent establishment of other land-uses). We used a space-for-time substitution sampling approach, measured SOC stocks in the top three meters of soil and compared recently converted land-uses with adjacent reference forest plots. In each respective region we investigated the most predominant land-use trajectories. In total 157 plots were established across the three countries, where soil samples were taken to a depth of three meters from a central soil pit and from the topsoil (to 0.5m) from 12 pooled composite samples. Finding 1 - soil depth: despite the fact that the majority of SOC stock from the three meter profile is found below one meter depth (50 to 60 percent of total SOC stock), the significant changes in SOC were only measured in the top meter of soil, while the subsoil carbon stock remained relatively unchanged by the land-use conversion. The only exception was for older (>50 yrs) cacao plantations in Cameroon where significant decreases were found below one meter. Finding 2 - pooled composite samples taken across the plot provided more spatially representative estimates of SOC stocks than samples taken from the central soil pit.

  17. Comparison of regional fat mass measurement by whole body DXA scans and anthropometric measures to predict insulin resistance in women with polycystic ovary syndrome and controls

    DEFF Research Database (Denmark)

    Glintborg, Dorte; Houborg Petersen, Maria; Ravn, Pernille

    2016-01-01

    INTRODUCTION: Polycystic ovary syndrome (PCOS) is characterized by obesity and insulin resistance. Measures of regional obesity may be used to predict insulin resistance. In the present study we compared fat distribution in patients with PCOS vs. controls and established the best measure of fat...

  18. Effect of the sample matrix on measurement uncertainty in X-ray fluorescence analysis

    International Nuclear Information System (INIS)

    Morgenstern, P.; Brueggemann, L.; Wennrich, R.

    2005-01-01

    The estimation of measurement uncertainty, with reference to univariate calibration functions, is discussed in detail in the Eurachem Guide 'Quantifying Uncertainty in Analytical Measurement'. The adoption of these recommendations to quantitative X-ray fluorescence analysis (XRF) involves basic problems which are above all due to the strong influence of the sample matrix on the analytical response. In XRF-analysis, the proposed recommendations are consequently applicable only to the matrix corrected response. The application is also restricted with regard to both the matrices and analyte concentrations. In this context the present studies are aimed at the problems to predict measurement uncertainty also with reference to more variable sample compositions. The corresponding investigations are focused on the use of the intensity of the Compton scattered tube line as an internal standard to assess the effect of the individual sample matrix on the analytical response relatively to a reference matrix. Based on this concept the estimation of the measurement uncertainty of an analyte presented in an unknown specimen can be predicted in consideration of the data obtained under defined matrix conditions

  19. Electrical resistivity and ultrasonic measurements during sequential fracture test of cementitious composite

    Directory of Open Access Journals (Sweden)

    V. Veselý

    2014-10-01

    Full Text Available Cracks in cover of reinforced and pre-stressed concrete structures significantly influence the ingress of deleterious species causing decrease in durability of these structures. The paper is focused on the effect of fracture process on two selected physical parameters of concrete – the electrical resistivity and the ultrasonic pulse passing time – which might be employed as the quality indicator of concrete cover within (nondestructive procedure(s of assessment of the structural durability. The concrete electrical resistivity and ultrasonic passing time were investigated here with respect to two variants of treatment of the test specimens’ surface (the pre-dried surface and the wet surface. Test configuration of three-point bending of notched beam was utilized to control the crack propagation; the fracture process passed through several loading–unloading sequences between which the electrical resistivity and ultrasonic passing time readings over the fractured region were performed. Equivalent elastic crack model was used for estimation of the fracture advance (described via the effective crack length at the loading stages corresponding to the resistivity and ultrasonic measurements. Relationships between changes of both the concrete resistivity and ultrasonic pulse passing time and the effective crack length is determined and discussed.

  20. Sensitivity of resistive and Hall measurements to local inhomogeneities: Finite-field, intensity, and area corrections

    Science.gov (United States)

    Koon, Daniel W.; Wang, Fei; Petersen, Dirch Hjorth; Hansen, Ole

    2014-10-01

    We derive exact, analytic expressions for the sensitivity of sheet resistance and Hall sheet resistance measurements to local inhomogeneities for the cases of nonzero magnetic fields, strong perturbations, and perturbations over a finite area, extending our earlier results on weak perturbations. We express these sensitivities for conductance tensor components and for other charge transport quantities. Both resistive and Hall sensitivities, for a van der Pauw specimen in a finite magnetic field, are a superposition of the zero-field sensitivities to both sheet resistance and Hall sheet resistance. Strong perturbations produce a nonlinear correction term that depends on the strength of the inhomogeneity. Solution of the specific case of a finite-sized circular inhomogeneity coaxial with a circular specimen suggests a first-order correction for the general case. Our results are confirmed by computer simulations on both a linear four-point probe array on a large circular disc and a van der Pauw square geometry. Furthermore, the results also agree well with Náhlík et al. published experimental results for physical holes in a circular copper foil disc.