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Sample records for reservoir-clark fork river

  1. South Fork Holston River basin 1988 biomonitoring

    Energy Technology Data Exchange (ETDEWEB)

    Saylor, C.F.; Ahlstedt, S.A.

    1990-06-01

    There is concern over the effects of shifts in land use use practices on the aquatic fauna of streams in the South Fork Holston River basin in northwestern North Carolina and southwestern Virginia. Trout reproduction has noticeably declined in the Watauga River subbasin. The Watauga River and Elk River subbasins have been subjected to commercial and resort development. The Middle fork Holston River and the upper South Fork Holston River subbasins have been affected by agricultural and mining activities, respectively (Cox, 1986). To aid reclamation and management of the South Fork Holston basin, Tennessee Valley Authority (TVA) biologists conducted biomonitoring--including index of biotic integrity and macroinvertebrate sampling--on the Middle Fork Holston, South Fork Holston, Watauga, and Elk Rivers to assess cumulative impairment related to changes in habitat and pollutant loading in these subbasins. Biomonitoring can detect environmental degradation, help document problem areas, and assist in development of strategies for managing water quality. This report discusses the methods and materials and results of the biomonitoring of South Fork Holston River Basin. 13 refs., 5 figs., 12 tabs.

  2. North Fork Feather River Erosion Control Program

    International Nuclear Information System (INIS)

    Harrison, L.

    1991-01-01

    PG and E, an investor owned gas and electric utility serving northern and central California, has been engaged since 1984 in the development and implementation of a regional erosion control program for the 954 square mile northern Sierra Nevada watersheed of the East Branch of the North Fork Feather River in Plumas County, California. PG and E entered into an agreement with 13 governmental agencies and a number of private landowners using Coordinated Resource Management and Planning: to cooperatively develop, fund and implement the program. The group has completed several field projects and has a number of additional projects in various stages of development. This paper reports that the program provides multiple environmental and economic benefits including reduction of soil erosion and sedimentation, improved fisheries, enhancement of riparian habitat, increased land values, improved recreation opportunities, and preservation of watershed resources

  3. 77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

    Science.gov (United States)

    2012-07-05

    ...-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining AGENCY: Forest Service, USDA... North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt River Mining Record of Decision only where necessary to address the inadequacies identified by the court...

  4. Archaeological Investigations on the East Fork of the Salmon River, Custer County, Idaho.

    Science.gov (United States)

    1984-01-01

    coniferous environment in addition to pine marten (Martes americana), red squirrel (Tamiasciurus hudsonicus), porcupine (Erithizon dorsatum), mountain vole...can be seen in small herds throughout the East Fork valley from the Salmon River to Big Boulder Creek. Two bands of Rocky Mountain bighorn sheep...utilize the Challis Planning Unit, one on the East Fork and the other in the Birch Creek area. The East Fork herd is comprised of approximately 50-70

  5. Channelization and floodplain forests: impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

    Science.gov (United States)

    Sonja N. Oswalt; Sammy L. King

    2005-01-01

    We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased...

  6. Integrating Salmon Recovery, Clean Water Act Compliance, Restoration, and Climate Change Impacts in the South Fork Nooksack River

    Science.gov (United States)

    "The South Fork Nooksack River (SFNR) is an important tributary to the Nooksack River, Bellingham Bay, and the Salish Sea. The South Fork Nooksack River comprises one of the 22 independent populations of spring Chinook in the Puget Sound Chinook Evolutionarily Significant Un...

  7. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Science.gov (United States)

    2010-07-01

    ... Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean... part. (2) No person or vessel may enter or navigate within this security zone unless authorized to do...

  8. South Fork Snake River/Palisades Wildlife Mitigation Project: Environmental assessment

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    BPA proposes to fund the implementation of the South Fork Snake River Programmatic Management Plan to compensate for losses of wildlife and wildlife habitat due to hydroelectric development at Palisades Dam. The Idaho Department of Fish and Game drafted the plan, which was completed in May 1993. This plan recommends land and conservation easement acquisition and wildlife habitat enhancement measures. These measures would be implemented on selected lands along the South Fork of the Snake River between Palisades Dam and the confluence with the Henry`s Fork, and on portions of the Henry`s Fork located in Bonneville, Madison, and Jefferson Counties, Idaho. BPA has prepared an Environmental Assessment evaluating the proposed project. The EA also incorporates by reference the analyses in the South Fork Snake River Activity/Operations Plan and EA prepared jointly in 1991 by the Bureau of Land Management and the Forest Service. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement (EIS) is not required and BPA is issuing this FONSI.

  9. 77 FR 47058 - Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft...

    Science.gov (United States)

    2012-08-07

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [P-2079-069--CA] Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft Environmental Impact Statement; Public Meetings a. Date and Time of Meetings: Tuesday, August 28, 2012, from 9:00 a.m. to 11:00 a.m. and...

  10. Flood-inundation maps for the East Fork White River at Columbus, Indiana

    Science.gov (United States)

    Lombard, Pamela J.

    2013-01-01

    Digital flood-inundation maps for a 5.4-mile reach of the East Fork White River at Columbus, Indiana, from where the Flatrock and Driftwood Rivers combine to make up East Fork White River to just upstream of the confluence of Clifty Creek with the East Fork White River, were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent of flooding corresponding to selected water levels (stages) at USGS streamgage 03364000, East Fork White River at Columbus, Indiana. Current conditions at the USGS streamgage may be obtained on the Internet from the USGS National Water Information System (http://waterdata.usgs.gov/in/nwis/uv/?site_no=03364000&agency_cd=USGS&). The National Weather Service (NWS) forecasts flood hydrographs for the East Fork White River at Columbus, Indiana at their Advanced Hydrologic Prediction Service (AHPS) flood warning system Website (http://water.weather.gov/ahps/), that may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. In this study, flood profiles were computed for the stream reach by means of a one-dimensional step-backwater model. The hydraulic model was calibrated by using the most current stage-discharge relation at USGS streamgage 03364000, East Fork White River at Columbus, Indiana. The calibrated hydraulic model was then used to determine 15 water-surface profiles for flood stages at 1-foot (ft) intervals referenced to the streamgage datum and ranging from bankfull to approximately the highest recorded water level at the streamgage. The simulated water-surface profiles were then combined with a geographic information system digital elevation model (derived from Light Detection and Ranging (LiDAR) data), having a 0.37-ft vertical accuracy and a 1.02 ft

  11. Flood-inundation maps for the East Fork White River near Bedford, Indiana

    Science.gov (United States)

    Fowler, Kathleen K.

    2014-01-01

    Digital flood-inundation maps for an 1.8-mile reach of the East Fork White River near Bedford, Indiana (Ind.) were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent and depth of flooding corresponding to selectedwater levels (stages) at USGS streamgage 03371500, East Fork White River near Bedford, Ind. Current conditions for estimating near-real-time areas of inundation using USGS streamgage information may be obtained on the Internet at http://waterdata.usgs.gov/in/nwis/uv?site_no=03371500. In addition, information has been provided to the National Weather Service (NWS) for incorporation into their Advanced Hydrologic Prediction Service (AHPS) flood warning system (http://water.weather.gov/ahps/). The NWS forecasts flood hydrographs at many places that are often colocated with USGS streamgages, including the East Fork White River near Bedford, Ind. NWS-forecasted peak-stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. For this study, flood profiles were computed for the East Fork White River reach by means of a one-dimensional step-backwater model. The hydraulic model was calibrated by using the most current stage-discharge relations at USGS streamgage 03371500, East Fork White River near Bedford, Ind., and documented high-water marks from the flood of June 2008. The calibrated hydraulic model was then used to determine 20 water-surface profiles for flood stages at 1-foot intervals referenced to the streamgage datum and ranging from bankfull to the highest stage of the current stage-discharge rating curve. The simulated water-surface profiles were then combined with a geographic information system (GIS) digital elevation model (DEM, derived from

  12. Spatial Patterns of Mercury Bioaccumulation in the Upper Clark Fork River Basin, MT

    Science.gov (United States)

    Staats, M. F.; Langner, H.; Moore, J. N.

    2010-12-01

    The Upper Clark Fork River Basin (UCFRB) in Montana has a legacy of historic gold/silver mine waste that contributes large quantities of mercury into the watershed. Mercury bioaccumulation at higher levels of the aquatic food chain, such as the mercury concentration in the blood of pre-fledge osprey, exhibit an irregular spatial signature based on the location of the nests throughout the river basin. Here we identify regions with a high concentration of bioavailable mercury and the major factors that allow the mercury to bioaccumulate within trophic levels. This identification is based on the abundance of mercury sources and the potential for mercury methylation. To address the source term, we did a survey of total mercury in fine sediments along selected UCFRB reaches, along with the assessment of environmental river conditions (percentage of backwaters/wetlands, water temperature and pH, etc). In addition, we analyzed the mercury levels of a representative number of macroinvertebrates and fish from key locations. The concentration of total mercury in sediment, which varies from reach to reach (tributaries of the Clark Fork River, 5mg/kg) affects the concentration of mercury found at various trophic levels. However, reaches with a low supply of mine waste-derived mercury can also yield substantial concentrations of mercury in the biota, due to highly favorable conditions for mercury methylation. We identify that the major environmental factor that affects the methylation potential in the UCFRB is the proximity and connectivity of wetland areas to the river.

  13. Hydrodynamics and Connectivity of Channelized Floodplains: Insights from the Meandering East Fork White River, Indiana, USA

    Science.gov (United States)

    Czuba, J. A.; David, S. R.; Edmonds, D. A.

    2017-12-01

    High resolution topography reveals that meandering river floodplains in Indiana commonly have networks of channels. These floodplain channel networks are most prevalent in agricultural, low-gradient, wide floodplains. It appears that these networks are formed when floodplain channels connect oxbows to each other and the main river channel. Collectively, the channels in the floodplain create an interconnected network of pathways that convey water beginning at flows less than bankfull, and as stage increases, more of the floodplain becomes dissected by floodplain channels. In this work, we quantify the hydrodynamics and connectivity of the flow on the floodplain and in the main channel of the East Fork White River near Seymour, Indiana, USA. We constructed a two-dimensional numerical model using HECRAS of the river-floodplain system from LiDAR data and from main-channel river bathymetry to elucidate the behaviour of these floodplain channels across a range of flows. Model calibration and verification data included stage from a USGS gage, high-water marks at a high and medium flow, and an aerial photograph of inundation in the floodplain channels. The numerical model simulated flow depth and velocity, which was used to quantify connectivity of the floodplain channels, exchange between the main channel and floodplain channels, and residence time of water on the floodplain. Model simulations suggest that the floodplain channels convey roughly 50% of the total flow at what is typically considered "bankfull" flow. Overall, we present a process-based approach for analyzing complex floodplain-river systems where an individual floodplain-river system can be distilled down to a set of characteristic curves. Notably, we map the East Fork White River system to exchange-residence time space and argue that this characterization forms the basis for thinking about morphologic evolution (e.g., sediment deposition and erosion) and biogeochemistry (e.g., nitrate removal) in floodplain-river

  14. Mercury and other Mining-Related Contaminants in Ospreys along the Upper Clark Fork River, MT

    Science.gov (United States)

    Langner, H.; Domenech, R.; Greene, E.; Staats, M. F.

    2010-12-01

    Osprey (Pandion haliaetus) are widely recognized as bio-indicators of the health of aquatic ecosystems. Until the time of fledging, nestlings feed exclusively on fish caught within a few kilometers of the nest. Therefore, tissues of these young birds may reflect the level of contamination of local fish and more generally, the contamination status of the aquatic ecosystem they inhabit. Nests can often be accessed with a boom truck and obtaining small blood samples from the flightless chicks is fairly noninvasive. Ospreys are nesting along the Upper Clark Fork River, Montana, which is heavily contaminated with wastes left from a century of copper and precious metals mining. We have been monitoring the levels of priority pollutants (arsenic, cadmium, lead, copper, zinc, mercury and selenium) in Osprey chicks along a 250 km section of the river for four years. Objectives are to establish current contaminant status, pinpoint pollution hotspots, and assess the success of restoration efforts. Our results suggest that of highest concern may be the bioaccumulation of mercury with blood levels of up to 0.7 mg/L in the growing chicks. These concentrations are expected to increase many fold upon fledging as feather growth stops, which acts as the major sink for mercury. Interestingly, we found mercury levels increased in downstream direction, in contrast to concentrations of other pollutants. Reasons may be the different origin of mercury versus other contaminants and the distribution of wetlands where mercury can be transformed into highly bioavailable methylmercury. Blood levels of selenium are also elevated throughout the Upper Clark Fork River drainage. We discuss the implications for restoration and remediation of the Clark Fork River.

  15. Geomorphic Change Induced by 100 years of Flow Alteration on the Diamond Fork River, Central Utah

    Science.gov (United States)

    Jones, J.; Belmont, P.; Wilcock, P. R.

    2017-12-01

    Changes in hydrology and sediment supply affect the form of rivers. The rate of change of fluvial form is controlled by a variety of factors, including valley confinement, sediment size, and antecedent condition. The Diamond Fork River in central Utah has been altered by trans-basin flows delivered from the Colorado River system for over a century. Beginning in 1915, water used for irrigation was delivered through a tributary, Sixth Water Creek, with daily summer flows regularly exceeding the 50 - 100 year flood. Elevated flows caused drastic geomorphic change - resulting in incision and widening of the channel, and the destruction of riparian vegetation. Beginning in 1997, the outlet for the trans-basin diversion was moved downstream on Sixth Water, bypassing a large landslide, and flows were drastically reduced in 2004 through management actions. We delineated eight distinct process domains for the Sixth Water-Diamond Fork system and examined the response of each process domain to the altered flow and sediment regimes through the analysis of aerial photographs and repeat cross-sections. We measured a variety of channel metrics, including channel width, areal extent of bars and islands, and sinuosity in ArcGIS. Results indicate that unconfined reaches that were wide and braided during the period of elevated flows have narrowed to become single threaded and meandering in response to the reduced flows. Confined reaches have experienced minor changes since the reduction in flows, suggesting that confinement is a primary control on the degree of channel response. These findings and complimentary studies will provide managers of Sixth Water and Diamond Fork with a greater understanding of the physical response of the streams, and the resulting effects on ecological communities.

  16. Hydrologic and hydraulic analyses for the Black Fork Mohican River Basin in and near Shelby, Ohio

    Science.gov (United States)

    Huitger, Carrie A.; Ostheimer, Chad J.; Koltun, G.F.

    2016-05-06

    Hydrologic and hydraulic analyses were done for selected reaches of five streams in and near Shelby, Richland County, Ohio. The U.S. Geological Survey (USGS), in cooperation with the Muskingum Watershed Conservancy District, conducted these analyses on the Black Fork Mohican River and four tributaries: Seltzer Park Creek, Seltzer Park Tributary, Tuby Run, and West Branch. Drainage areas of the four stream reaches studied range from 0.51 to 60.3 square miles. The analyses included estimation of the 10-, 2-, 1-, and 0.2-percent annual-exceedance probability (AEP) flood-peak discharges using the USGS Ohio StreamStats application. Peak discharge estimates, along with cross-sectional and hydraulic structure geometries, and estimates of channel roughness coefficients were used as input to step-backwater models. The step-backwater water models were used to determine water-surface elevation profiles of four flood-peak discharges and a regulatory floodway. This study involved the installation of, and data collection at, a streamflow-gaging station (Black Fork Mohican River at Shelby, Ohio, 03129197), precipitation gage (Rain gage at Reservoir Number Two at Shelby, Ohio, 405209082393200), and seven submersible pressure transducers on six selected river reaches. Two precipitation-runoff models, one for the winter events and one for nonwinter events for the headwaters of the Black Fork Mohican River, were developed and calibrated using the data collected. With the exception of the runoff curve numbers, all other parameters used in the two precipitation-runoff models were identical. The Nash-Sutcliffe model efficiency coefficients were 0.737, 0.899, and 0.544 for the nonwinter events and 0.850 and 0.671 for the winter events. Both of the precipitation-runoff models underestimated the total volume of water, with residual runoff ranging from -0.27 inches to -1.53 inches. The results of this study can be used to assess possible mitigation options and define flood hazard areas that

  17. Mapping Prehistoric, Historic, and Channel Sediment Distribution, South Fork Noyo River: A Tool For Understanding Sources, Storage, and Transport

    Science.gov (United States)

    Rich D. Koehler; Keith I. Kelson; Graham Matthews; K.H. Kang; Andrew D. Barron

    2007-01-01

    The South Fork Noyo River (SFNR) watershed in coastal northern California contains large volumes of historic sediment that were delivered to channels in response to past logging operations. This sediment presently is stored beneath historic terraces and in present-day channels. We conducted geomorphic mapping on the SFNR valley floor to assess the volume and location...

  18. 75 FR 41237 - Public Land Order No. 7746; Withdrawal of Public Lands, South Fork of the American River; California

    Science.gov (United States)

    2010-07-15

    ... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLCAC08000-L1430000-ET0000; CACA 41334] Public Land Order No. 7746; Withdrawal of Public Lands, South Fork of the American River; California AGENCY: Bureau of Land Management, Interior. ACTION: Public Land Order. SUMMARY: This order withdraws 2...

  19. South Fork Salmon River Watershed Restoration, 2008-2009 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Reaney, Mark D. [Nez Perce Tribe Department of Fisheries Resource Management

    2009-04-15

    The watershed restoration work elements within the project area, the South Fork Salmon River Watershed, follow the watershed restoration approach adopted by the Nez Perce Tribe Department of Fisheries Resource Management (DFRM) - Watershed Division. The vision of the Nez Perce Tribe DFRM-Watershed Division focuses on protecting, restoring, and enhancing watersheds and treaty resources within the ceded territory of the Nez Perce Tribe under the Treaty of 1855 with the United States Federal Government. The program uses a holistic approach, which encompasses entire watersheds, ridge top to ridge top, emphasizing all cultural aspects and strategies that rely on natural fish production and healthy river ecosystems. The Nez Perce Tribe DFRM-Watershed Division strives towards maximizing historic ecosystem productivity and health for the restoration of anadromous and resident fish populations and the habitat on which all depend on for future generations Originally, this project was funded to create a step/pool stream channel that was appropriate to restore fish passage where the 'Glory Hole Cascade' is currently located at the Stibnite Mine. Due to unforeseen circumstances at the time, the project is unable to move forward as planned and a request for a change in scope of the project and an expansion of the geographic area in which to complete project work was submitted. No additional funds were being requested. The ultimate goal of this project is to work with the holistic, ridge top to ridge top approach to protect and restore the ecological and biological functions of the South Fork Salmon River Watershed to assist in the recovery of threatened and endangered anadromous and resident fish species. FY 2008 Work Elements included two aquatic organism passage (AOP) projects to restore habitat connectivity to two fish-bearing tributaries to the East Fork South Fork Salmon River, Salt and Profile Creeks. The Work Elements also included road survey and assessment

  20. Qualitative Assessment: Evaluating the Impacts of Climate Change on Endangered Species Act Recovery Actions for the South Fork Nooksack River, WA

    Science.gov (United States)

    The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South F...

  1. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS, AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    Science.gov (United States)

    This technical report provides a description of the field project design, quality control, the sampling protocols and analysis methodology used, and standard operating procedures for the South Fork Broad River Watershed (SFBR) Total Maximum Daily Load (TMDL) project. This watersh...

  2. Bed-material entrainment potential, Roaring Fork River at Basalt, Colorado

    Science.gov (United States)

    Elliott, John G.

    2002-01-01

    The Roaring Fork River at Basalt, Colorado, has a frequently mobile streambed composed of gravel, cobbles, and boulders. Recent urban and highway development on the flood plain, earlier attempts to realign and confine the channel, and flow obstructions such as bridge openings and piers have altered the hydrology, hydraulics, sediment transport, and sediment deposition areas of the Roaring Fork. Entrainment and deposition of coarse sediment on the streambed and in large alluvial bars have reduced the flood-conveying capacity of the river. Previous engineering studies have identified flood-prone areas and hazards related to inundation and high streamflow velocity, but those studies have not evaluated the potential response of the channel to discharges that entrain the coarse streambed. This study builds upon the results of earlier flood studies and identifies some potential areas of concern associated with bed-material entrainment. Cross-section surveys and simulated water-surface elevations from a previously run HEC?RAS model were used to calculate the boundary shear stress on the mean streambed, in the thalweg, and on the tops of adjacent alluvial bars for four reference streamflows. Sediment-size characteristics were determined for surficial material on the streambed, on large alluvial bars, and on a streambank. The median particle size (d50) for the streambed samples was 165 millimeters and for the alluvial bars and bank samples was 107 millimeters. Shear stresses generated by the 10-, 50-, and 100-year floods, and by a more common flow that just inundated most of the alluvial bars in the study reach were calculated at 14 of the cross sections used in the Roaring Fork River HEC?RAS model. The Shields equation was used with a Shields parameter of 0.030 to estimate the critical shear stress for entrainment of the median sediment particle size on the mean streambed, in the thalweg, and on adjacent alluvial bar surfaces at the 14 cross sections. Sediment

  3. Hydrogeology and simulated groundwater flow and availability in the North Fork Red River aquifer, southwest Oklahoma, 1980–2013

    Science.gov (United States)

    Smith, S. Jerrod; Ellis, John H.; Wagner, Derrick L.; Peterson, Steven M.

    2017-09-28

    On September 8, 1981, the Oklahoma Water Resources Board established regulatory limits on the maximum annual yield of groundwater (343,042 acre-feet per year) and equal-proportionate-share (EPS) pumping rate (1.0 acre-foot per acre per year) for the North Fork Red River aquifer. The maximum annual yield and EPS were based on a hydrologic investigation that used a numerical groundwater-flow model to evaluate the effects of potential groundwater withdrawals on groundwater availability in the North Fork Red River aquifer. The Oklahoma Water Resources Board is statutorily required (every 20 years) to update the hydrologic investigation on which the maximum annual yield and EPS were based. Because 20 years have elapsed since the final order was issued, the U.S. Geological Survey, in cooperation with the Oklahoma Water Resources Board, conducted an updated hydrologic investigation and evaluated the effects of potential groundwater withdrawals on groundwater flow and availability in the North Fork Red River aquifer in Oklahoma. This report describes a hydrologic investigation of the North Fork Red River aquifer that includes an updated summary of the aquifer hydrogeology. As part of this investigation, groundwater flow and availability were simulated by using a numerical groundwater-flow model.The North Fork Red River aquifer in Beckham, Greer, Jackson, Kiowa, and Roger Mills Counties in Oklahoma is composed of about 777 square miles (497,582 acres) of alluvium and terrace deposits along the North Fork Red River and tributaries, including Sweetwater Creek, Elk Creek, Otter Creek, and Elm Fork Red River. The North Fork Red River is the primary source of surface-water inflow to Lake Altus, which overlies the North Fork Red River aquifer. Lake Altus is a U.S. Bureau of Reclamation reservoir with the primary purpose of supplying irrigation water to the Lugert-Altus Irrigation District.A hydrogeologic framework was developed for the North Fork Red River aquifer and included a

  4. Assessing the potential for rainbow trout reproduction in tributaries of the Mountain Fork River below Broken Bow Dam, southeastern Oklahoma

    Science.gov (United States)

    Long, James M.; Starks, Trevor A.; Farling, Tyler; Bastarache, Robert

    2016-01-01

    Stocked trout (Salmonidae) in reservoir tailwater systems in the Southern United States have been shown to use tributary streams for spawning and rearing. The lower Mountain Fork of the Little River below Broken Bow Dam is one of two year-round tailwater trout fisheries in Oklahoma, and the only one with evidence of reproduction by stocked rainbow trout (Oncorhynchus mykiss). Whether stocked trout use tributaries in this system for spawning is unknown. Furthermore, an

  5. Fight and air exposure times of caught and released salmonids from the South Fork Snake River

    Science.gov (United States)

    Roth, Curtis J.; Schill, Daniel J.; Quist, Michael C.

    2018-01-01

    Catch-and-release regulations are among the most common types of fishing regulations. In recent years, concerns have arisen regarding the exposure of fish to air during catch-and-release angling. The purpose of our study was to quantify the length of time angled fish were exposed to air by anglers in a typical catch-and-release fishery and relate it to the lengths of time reported to produce negative effects. In total, 312 individual anglers were observed on the South Fork Snake River, Idaho, from May through August 2016. Fight time varied from 1.1 s to 230.0 s, and average fight time was 40.0 s (SD = 36.8). Total air exposure times varied from 0.0 s to 91.8 s and averaged 19.3 s (SD = 15.0). Though not statistically significant, a trend in reduced fight times was observed when anglers were guided and increased air exposure times when a net was used and a picture was taken. Results of the current study suggest that anglers expose fish to air for periods that are much less than those reported to cause mortality.

  6. Floodplain Vegetation Productivity and Carbon Cycle Dynamics of the Middle Fork Flathead River of Northwest Montana

    Science.gov (United States)

    Oakins, A. J.; Kimball, J. S.; Relyea, S.; Stanford, J. A.

    2005-05-01

    River floodplains are vital natural features that store floodwaters, improve water quality, provide habitat, and create recreational opportunities. Recent studies have shown that strong interactions among flooding, channel and sediment movement, vegetation, and groundwater create a dynamic shifting habitat mosaic that promotes biodiversity and complex food webs. Multiple physical and environmental processes interact within these systems to influence forest productivity, including water availability, nutrient supply, soil texture, and disturbance history. This study is designed to quantify the role of groundwater depth and meteorology in determining spatial and temporal patterns of net primary productivity (NPP) within the Nyack floodplain of the Middle Fork Flathead River, Northwestern Montana. We examine three intensive field sites composed of mature, mixed deciduous and evergreen conifer forest with varying hydrologic and vegetative characteristics. We use a modified Biome-BGC ecosystem process model with field-collected data (LAI, increment growth cores, groundwater depth, vegetation sap-flow, and local meteorology) to describe the effects of floodplain groundwater dynamics on vegetation community structure, and carbon/nitrogen cycling. Initial results indicate that conifers are more sensitive than deeper-rooted deciduous species to variability in groundwater depth and meteorological conditions. Forest productivity also shows a non-linear response to groundwater depth. Sites with intermediate groundwater depths (0.2-0.5m) allow vegetation to maintain connectivity to groundwater over longer periods during the growing season, are effectively uncoupled from atmospheric constraints on photosynthesis, and generally have greater productivity. Shallow groundwater sites (<0.2m) are less productive due to the indirect effects of reduced soil aerobic decomposition and reduced plant available nitrogen.

  7. Status of the dirty darter, Etheostoma olivaceum, and bluemask darter, Etheostoma (Doration)sp., with notes on fishes of the Caney Fork River system, Tennessee

    International Nuclear Information System (INIS)

    Layman, S.R.; Simons, A.M.; Wood, R.M.

    1993-01-01

    Seventy-six localities were sampled in the Caney Fork River system and adjacent Cumberland River tributaries. Etheostoma olivaceum was found in small creeks from nine tributaries of lower Caney Fork River and three tributaries of the Cumberland River in the Nashville Basin physiographic province. The species was most abundant around slab rocks and rubble over bedrock in slow to moderate current. Etheostoma olivaceum was common throughout its small range; however, given widespread habitat degradation from agriculture, the species should retain its open-quotes deemed in need of managementclose quotes status in Tennessee. The bluemask darter, Etheostoma (Doration) sp., was collected in slow to moderate current over sand and gravel in Collins River, Rocky River, Cane Creek, and Caney Fork River. All four populations were isolated upstream of Great Falls Reservoir in the Highland Rim physiographic province. The species was found in a 37-km reach of Collins River but was restricted to reaches of 0.2 to 4.3 km in the other three streams. Threats to the species include pesticides from plant nurseries, siltation, gravel dredging, and acid mine drainage. The authors recommend that the bluemask darter be listed as state and federally protected. Two new records were established for the rare Barrens darter, Etheostoma forbesi, in lower Collins River and Barren Fork River, and eight previously unknown records of the species were identified from older museum collections. 21 refs., 1 fig., 1 tab

  8. Metamorphism and plutonism around the middle and south forks of the Feather River, California

    Science.gov (United States)

    Hietanen, Anna Martta

    1976-01-01

    The area around the Middle and South Forks of the Feather River provides information on metamorphic and igneous processes that bear on the origin of andesitic and granitic magmas in general and on the variation of their potassium content in particular. In the north, the area joins the Pulga and Bucks Lake quadrangles studied previously. Tectonically, this area is situated in the southern part of an arcuate segment of the Nevadan orogenic belt in the northwestern Sierra Nevada. The oldest rocks are metamorphosed calcalkaline island-arc-type andesite, dacite, and sodarhyolite with interbedded tuff layers (the Franklin Canyon Formation), all probably correlative with Devonian rocks in the Klamath Mountains. Younger rocks form a sequence of volcanic, volcaniclastic, and sedimentary rocks including some limestone (The Horseshoe Bend Formation), probably Permian in age. All the volcanic and sedimentary rocks were folded and recrystallized to the greenschist facies during the Nevadan (Jurassic) orogeny and were invaded by monzotonalitic magmas shortly thereafter. A second lineation and metamorphism to the epidote-amphibolite facies developed in a narrow zone around the plutons. In light of the concept of plate tectonics, it is suggested that the early (Devonian?) island-arc-type andesite, dacite, and sodarhyolite (the Franklin Canyon Formation) were derived from the mantle above a Benioff zone by partial melting of peridotite in hydrous conditions. The water was probably derived from an oceanic plate descending to the mantle. Later (Permian?) magmas were mainly basaltic; some discontinuous layers of potassium-rich rhyolite indicate a change into anhydrous conditions and a deeper level of magma generation. The plutonic magmas that invaded the metamorphic rocks at the end of the Jurassic may contain material from the mantle, the subducted oceanic lithosphere, and the downfolded metamorphic rocks. The ratio of partial melts from these three sources may have changed with time

  9. Flood-inundation maps for an 8.9-mile reach of the South Fork Little River at Hopkinsville, Kentucky

    Science.gov (United States)

    Lant, Jeremiah G.

    2013-01-01

    Digital flood-inundation maps for an 8.9-mile reach of South Fork Little River at Hopkinsville, Kentucky, were created by the U.S. Geological Survey (USGS) in cooperation with the City of Hopkinsville Community Development Services. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage at South Fork Little River at Highway 68 By-Pass at Hopkinsville, Kentucky (station no. 03437495). Current conditions for the USGS streamgage may be obtained online at the USGS National Water Information System site (http://waterdata.usgs.gov/nwis/inventory?agency_code=USGS&site_no=03437495). In addition, the information has been provided to the National Weather Service (NWS) for incorporation into their Advanced Hydrologic Prediction Service flood warning system (http://water.weather.gov/ahps/). The NWS forecasts flood hydrographs at many places that are often co-located at USGS streamgages. The forecasted peak-stage information, also available on the Internet, may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. In this study, flood profiles were computed for the South Fork Little River reach by using HEC-RAS, a one-dimensional step-backwater model developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated by using the most current (2012) stage-discharge relation at the South Fork Little River at Highway 68 By-Pass at Hopkinsville, Kentucky, streamgage and measurements collected during recent flood events. The calibrated model was then used to calculate 13 water-surface profiles for a sequence of flood stages, most at 1-foot intervals, referenced to the streamgage datum and ranging from a stage near bank full to the estimated elevation of the 1.0-percent annual exceedance

  10. Historical perspectives on channel pattern in the Clark Fork River, Montana and implications for post-dam removal restoration

    Science.gov (United States)

    Woelfle-Erskine, C. A.; Wilcox, A. C.

    2009-12-01

    Active restoration approaches such as channel reconstruction have moved beyond the realm of small streams and are being applied to larger rivers. Uncertainties arising from limited knowledge, fluvial and ecosystem variability, and contaminants are especially significant in restoration of large rivers, where project costs and the social, infrastructural, and ecological costs of failure are high. We use the case of Milltown Dam removal on the Clark Fork River, Montana and subsequent channel reconstruction in the former reservoir to examine the use of historical research and uncertainty analysis in river restoration. At a cost of approximately $120 million, the Milltown Dam removal involves the mechanical removal of approximately 2 million cubic meters of sediments contaminated by upstream mining, followed by restoration of the former reservoir reach in which a single-thread meandering channel is being constructed. Historical maps, surveys, photographs, and accounts suggest a conceptual model of a multi-thread, anastomosing river in the reach targeted for channel reconstruction, upstream of the confluence of the Clark Fork and Blackfoot Rivers. We supplemented historical research with analysis of aerial photographs, topographic data, and USGS stage-discharge measurements in a lotic but reservoir-influenced reach of the Clark Fork River within our study area to estimate avulsion frequency (0.8 avulsions/year over a 70-year period) and average rates of lateral migration and aggradation. These were used to calculate the mobility number, a dimensionless relationship between channel filling and lateral migration timescales that can be used to predict whether a river’s planform is single or multi-threaded. The mobility number within our study reach ranged from 0.6 (multi-thread channel) to 1.7 (transitional channel). We predict that, in the absence of active channel reconstruction, the post-dam channel pattern would evolve to one that alternates between single and multi

  11. Twelve Years of Monitoring Phosphorus and Suspended-Solids Concentrations and Yields in the North Fork Ninnescah River above Cheney Reservoir, South-Central Kansas 1997-2008

    Science.gov (United States)

    Stone, Mandy L.; Graham, Jennifer L.; Ziegler, Andrew C.

    2009-01-01

    Cheney Reservoir, located on the North Fork Ninnescah River in south-central Kansas, is the primary water supply for the city of Wichita and an important recreational resource. Concerns about taste-and-odor occurrences in Cheney Reservoir have drawn attention to potential pollutants, including total phosphorus (TP) and total suspended solids (TSS). July 2009 was the 15th anniversary of the establishment of the Cheney Reservoir Watershed pollution management plan. The U.S. Geological Survey (USGS), in cooperation with the city of Wichita, has collected water-quality data in the basin since 1996, and has monitored water quality continuously on the North Fork Ninnescah River since 1998. This fact sheet describes 12 years (1997-2008) of computed TP and TSS data and compares these data with water-quality goals for the North Fork Ninnescah River, the main tributary to Cheney Reservoir.

  12. Mercury Contributions from Flint Creek and other Tributaries to the Upper Clark Fork River in Northwestern Montana

    Science.gov (United States)

    Langner, H.; Young, M.; Staats, M. F.

    2013-12-01

    Methylmercury contamination in biota is a major factor diminishing the environmental quality of the Upper Clark Fork River (CFR), e.g. by triggering human consumption limits of fish. The CFR is subject to one of the largest Superfund cleanup projects in the US, but remediation and restoration is currently focused exclusively on other mining-related contaminants (As, Cu, Zn, Pb, Cd), which may be counterproductive with respect to the bio-availability of mercury, for example by creation of wetlands along mercury-contaminated reaches of the river. The identification and elimination of Hg sources is an essential step toward reducing the methylmercury exposure in the biota of the CFR watershed because a strong correlation exists between total mercury levels in river sediment and methylmercury levels in aquatic life. We analyzed duplicate samples from the top sediment layer of the main stem and significant tributaries to the Clark Fork River along a 240 km reach between Butte, MT and downstream of the Missoula Valley. Mercury concentrations were 1.3 × 1.6 (mean × SD, n = 35) in the main stem. Concentrations in tributaries varied widely (0.02 to 85 mg/kg) and seemed only loosely related to the number of historic precious metal mines in the watershed. In the upper reach of the CFR, elevated Hg levels are likely caused by residual contaminated sediments in the flood plain. Levels tend to decrease downstream until Drummond, MT, where Flint Creek contributes a significant amount of mercury, causing Hg levels in the main stem CFR to increase from 0.7 to 4 mg/kg. Levels continue to decrease downstream. Flint Creek is the single largest contributor of Hg to the CFR. Detailed sampling of the main stem Flint Creek and tributaries (26 sites) showed extremely high levels in two tributaries (22 to 85 mg/kg) where historic milling operations were located. Elimination of these point sources may be accomplished comparatively economically and may significantly reduce mercury levels in

  13. Stream bank and sediment movement associated with 2008 flooding, South Fork Iowa River

    Science.gov (United States)

    Stream bank erosion can cause substantial damage to riparian systems and impact the use of water downstream. Risks of bank erosion increase during extreme flood events, and frequencies of extreme events may be increasing under changing climate. We assessed bank erosion within the South Fork Iowa Riv...

  14. Role of river flow and sediment mobilization in riparian alder establishment along a bedrock-gravel river, South Fork Eel River, California

    Science.gov (United States)

    Jablkowski, P.; Johnson, E. A.; Martin, Y. E.

    2017-10-01

    Climatic, hydraulics, hydrologic, and fluvial geomorphic processes are the main drivers of riparian white alder (Alnus rhombifolia Nutt.) distribution in northern California. The Mediterranean climate and canyon bound, bedrock-gravel morphology of the South Fork Eel have a distinct effect on these processes. White alder seeds are preferentially deposited on river bars where river hydraulics create eddies coinciding with the downstream part of riffles and the upstream part of pools. Seeds are generally deposited below bankfull elevations by the descending hydrograph during the spring season in this Mediterranean climate. For successful germination and establishment, the seeds must be deposited at a location such that they are not remobilized by late spring flows. The summer establishment period is defined from the date of seed deposition and germination to the fall/winter date of river sediment mobilization. Seedling root growth rate decreases exponentially with decreasing water potential. However, seedlings are shown not to be generally limited by water availability at the elevations they are most commonly deposited. The establishment of white alder seedlings following the first summer will therefore depend on their ability to resist fall/winter high flows. The method proposed here compares the predicted rooting depth to predicted sediment scour rates. The length of the establishment period rather than water availability determines final seedling rooting depth. Over the past 40 years, very few years had establishment periods that were long enough or had fast enough alder growth rates to survive winter floods that often scour deeper than the total root length. The low survival of seedlings in the first autumn season following germination is believed to be a principal reason for the missing age classes often found in alder distributions along rivers.

  15. The ophiolitic North Fork terrane in the Salmon River region, central Klamath Mountains, California

    Science.gov (United States)

    Ando, C.J.; Irwin, W.P.; Jones, D.L.; Saleeby, J.B.

    1983-01-01

    The North Fork terrane is an assemblage of ophiolitic and other oceanic volcanic and sedimentary rocks that has been internally imbricated and folded. The ophiolitic rocks form a north-trending belt through the central part of the region and consist of a disrupted sequence of homogeneous gabbro, diabase, massive to pillowed basalt, and interleaved tectonitic harzburgite. U-Pb zircon age data on a plagiogranite pod from the gabbroic unit indicate that at least this part of the igneous sequence is late Paleozoic in age.The ophiolitic belt is flanked on either side by mafic volcanic and volcaniclastic rocks, limestone, bedded chert, and argillite. Most of the chert is Triassic, including much of Late Triassic age, but chert with uncertain stratigraphic relations at one locality is Permian. The strata flanking the east side of the ophiolitic belt face eastward, and depositional contacts between units are for the most part preserved. The strata on the west side of the ophiolitic belt are more highly disrupted than those on the east side, contain chert-argillite melange, and have unproven stratigraphic relation to either the ophiolitic rocks or the eastern strata.Rocks of the North Fork terrane do not show widespread evidence of penetrative deformation at elevated temperatures, except an early tectonitic fabric in the harzburgite. Slip-fiber foliation in serpentinite, phacoidal foliation in chert and mafic rocks, scaly foliation in argillite, and mesoscopic folds in bedded chert are consistent with an interpretation of large-scale anti-formal folding of the terrane about a north-south hinge found along the ophiolitic belt, but other structural interpretations are tenable. The age of folding of North Fork rocks is constrained by the involvement of Triassic and younger cherts and crosscutting Late Jurassic plutons. Deformation in the North Fork terrane must have spanned a short period of time because the terrane is bounded structurally above and below by Middle or Late

  16. Habitat selection influences sex distribution, morphology, tissue biochemistry, and parasite load of juvenile coho salmon in the West Fork Smith River, Oregon

    Science.gov (United States)

    Given the strong influence of water temperature on salmonid physiology and behavior, in the summers of 2004 and 2005 we studied juvenile male and female coho salmon Oncorhynchus kisutch in two reaches of Oregon’s West Fork Smith River with different thermal profiles. Our goals we...

  17. Effects of catastrophic floods and debris flows on the sediment retention structure, North Fork Toutle River, Washington

    Science.gov (United States)

    Denlinger, Roger P.

    2012-01-01

    The eruption of Mount St. Helens in 1980 produced a debris avalanche that flowed down the upper reaches of the North Fork Toutle River in southwestern Washington, clogging this drainage with sediment. In response to continuous anomalously high sediment flux into the Toutle and Cowlitz Rivers resulting from this avalanche and associated debris flows, the U.S. Army Corps of Engineers completed a Sediment Retention Structure (SRS) on the North Fork Toutle River in May 1989. For one decade, the SRS effectively blocked most of the sediment transport down the Toutle River. In 1999, the sediment level behind the SRS reached the elevation of the spillway base. Since then, a higher percentage of sediment has been passing the SRS and increasing the flood risk in the Cowlitz River. Currently (2012), the dam is filling with sediment at a rate that cannot be sustained for its original design life, and the U.S. Army Corps of Engineers is concerned with the current ability of the SRS to manage floods. This report presents an assessment of the ability of the dam to pass large flows from three types of scenarios (it is assumed that no damage to the spillway will occur). These scenarios are (1) a failure of the debris-avalanche blockage forming Castle Lake that produces a dambreak flood, (2) a debris flow from failure of that blockage, or (3) a debris flow originating in the crater of Mount St. Helens. In each case, the flows are routed down the Toutle River and through the SRS using numerical models on a gridded domain produced from a digital elevation model constructed with existing topography and dam infrastructure. The results of these simulations show that a structurally sound spillway is capable of passing large floods without risk of overtopping the crest of the dam. In addition, large debris flows originating from Castle Lake or the crater of Mount St. Helens never reach the SRS. Instead, debris flows fill the braided channels upstream of the dam and reduce its storage

  18. Hydraulic, geomorphic, and trout habitat conditions of the Lake Fork of the Gunnison River in Hinsdale County, Lake City, Colorado, Water Years 2010-2011

    Science.gov (United States)

    Williams, Cory A.; Richards, Rodney J.; Schaffrath, Keelin R.

    2015-01-01

    Channel rehabilitation, or reconfiguration, to mitigate a variety of riverine problems has become a common practice in the western United States. However, additional work to monitor and assess the channel response to, and the effectiveness of, these modifications over longer periods of time (decadal or longer) is still needed. The Lake Fork of the Gunnison River has been an area of active channel modification to accommodate the needs of the Lake City community since the 1950s. The Lake Fork Valley Conservancy District began a planning process to assess restoration options for a reach of the Lake Fork in Lake City to enhance hydraulic and ecologic characteristics of the reach. Geomorphic channel form is affected by land-use changes within the basin and geologic controls within the reach. The historic channel was defined as a dynamic, braided channel with an active flood plain. This can result in a natural tendency for the channel to braid. A braided channel can affect channel stability of reconfigured reaches when a single-thread meandering channel is imposed on the stream. The U.S. Geological Survey, in cooperation with the Colorado Water Conservation Board and Colorado River Water Conservation District, began a study in 2010 to quantify existing hydraulic and habitat conditions for a reach of the Lake Fork of the Gunnison River in Lake City, Colorado. The purpose of this report is to quantify existing Lake Fork hydraulic and habitat conditions and establish a baseline against which post-reconfiguration conditions can be compared. This report (1) quantifies the existing hydraulic and geomorphic conditions in a 1.1-kilometer section of the Lake Fork at Lake City that has been proposed as a location for future channel-rehabilitation efforts, (2) characterizes the habitat suitability of the reach for two trout species based on physical conditions within the stream, and (3) characterizes the current riparian canopy density.

  19. Organic compounds in Elm Fork Trinity River water used for public supply near Carrollton, Texas, 2002-05

    Science.gov (United States)

    Ging, Patricia B.; Delzer, Gregory C.; Hamilton, Pixie A.

    2009-01-01

    Organic compounds studied in this U.S. Geological Survey (USGS) assessment generally are man-made, including pesticides, solvents, gasoline hydrocarbons, personal-care and domestic-use products, refrigerants, and propellants. A total of 103 of 277 compounds were detected at least once among the 30 samples of source water for a community water system on the Elm Fork Trinity River near Carrollton, Texas, collected approximately monthly during 2002-05. The diversity of compounds detected indicates a variety of different sources and uses (including wastewater discharge, industrial, agricultural, domestic, and others) and different pathways (including overland runoff and groundwater discharge) to drinking-water supplies. Nine compounds were detected year-round in source-water samples, including chloroform, methyl tert-butyl ether (MTBE), and selected herbicide compounds commonly used in the Trinity River Basin and in other urban areas across the United States. About 90 percent of the 42 compounds detected most frequently in source water (in at least 20 percent of the samples) also were detected most frequently in finished water (after treatment but before distribution). Concentrations for all detected compounds in source and finished water generally were less than 0.1 microgram per liter and always less than human-health benchmarks, which are available for about one-half of the detected compounds.

  20. Hydrology and model of North Fork Solomon River Valley, Kirwin Dam to Waconda Lake, north-central Kansas

    Science.gov (United States)

    Jorgensen, Donald G.; Stullken, Lloyd E.

    1981-01-01

    The alluvial valley of the North Fork Solomon River is an important agricultural area. Reservoir releases diverted below Kirwin Dam are the principal source of irrigation water. During the 1970'S, severe water shortages occurred in Kirwin Reservoir and other nearby reservoirs as a result of an extended drought. Some evidence indicates that surface-water shortages may have been the result of a change in the rainfall-runoff relationship. Examination of the rainfall-runoff relationship shows no apparent trend from 1951 to 1968, but annual records from 1969 to 1976 indicate that deficient rainfall occurred during 6 of the 8 years. Ground water from the alluvial aquifer underlying the river valley also is used extensively for irrigation. Utilization of ground water for irrigation greatly increased from about 200 acre-feet in 1955 to about 12,300 acre-feet in 1976. Part of the surface water diverted for irrigation has percolated downward into the aquifer raising the ground-water level. Ground-water storage in the aquifer increased from 230,000 acre-feet in 1946 to 275,000 acre-feet in 1976-77. A digital model was used to simulate the steady-state conditions in the aquifer prior to closure of Kirwin Dam. Model results indicated that precipitation was the major source of recharge to the aquifer. The effective recharge, or gain from precipitation minus evapotranspiration, was about 11,700 acre-feet per year. The major element of discharge from the aquifer was leakage to the river. The simulated net leakage (leakage to the river minus leakage from the river) was about 11,500 acre-feet per year. The simulated value is consistent with the estimated gain in base flow of the river within the area modeled. Measurements of seepage used to determine gain and loss to the stream were made twice during 1976. Based on these measurements and on base-flow periods identified from hydrographs, it was estimated that the ground-water discharge to the stream has increased about 4,000 acre

  1. Geomorphic response of the North Fork Stillaguamish River to the State Route 530 landslide near Oso, Washington

    Science.gov (United States)

    Anderson, Scott W.; Keith, Mackenzie K.; Magirl, Christopher S.; Wallick, J. Rose; Mastin, Mark C.; Foreman, James R.

    2017-08-03

    On March 22, 2014, the State Route 530 Landslide near Oso, Washington mobilized 8 million cubic meters of unconsolidated Pleistocene material, creating a valley‑spanning deposit that fully impounded the North Fork Stillaguamish River. The river overtopped the 8-meter high debris impoundment within 25 hours and began steadily incising a new channel through the center of the deposit. Repeat topographic surveys, sediment transport measurements, bedload transport models, and observations of downstream channel change were used to document the establishment of that new channel through the landslide and assess the potential for downstream aggradation or channel change that might increase downstream flood hazards.Efficient erosion of the landslide deposit, associated with the steep knickzone formed by the downstream edge of the deposit, resulted in the re-establishment of a 20–40 meters wide, deeply inset channel through the entire deposit by May 2014, 2 months after the landslide. The mean water-surface elevation of the channel through the landslide decreased 7 meters during that 2-month period, and was about 1 meter above the pre-landslide profile in July 2014. The 2014–15 flood season, which included flows near the 0.5 annual exceedance probability discharge (2-year flood), widened the channel tens of meters, and further lowered the water-surface profile 0.5 meter. The planform position evolved slowly as a result of 5–20-meter high banks predominantly composed of clay-rich, cohesive lacustrine material. Erosion of the landslide deposit delivered a total of 820 thousand metric tons of sediment to the North Fork Stillaguamish River over the 18 months following the landslide. The sediment delivery from the deposit was predominantly fine grained: 77 percent (by mass) of the eroded material was silt or clay (less than 0.063 millimeter [mm]), 19 percent sand (0.063–2 mm), and 4 percent pebbles and cobbles (greater than 2 mm).Over the 18 months following the

  2. Development of CE-QUAL-W2 models for the Middle Fork Willamette and South Santiam Rivers, Oregon

    Science.gov (United States)

    Buccola, Norman L.; Stonewall, Adam J.; Sullivan, Annett B.; Kim, Yoonhee; Rounds, Stewart A.

    2013-01-01

    Hydrodynamic (CE-QUAL-W2) models of Hills Creek Lake (HCL), Lookout Point Lake (LOP), and Dexter Lake (DEX) on the Middle Fork Willamette River (MFWR), and models of Green Peter Lake and Foster Lake on the South Santiam River systems in western Oregon were updated and recalibrated for a wide range of flow and meteorological conditions. These CE-QUAL-W2 models originally were developed by West Consultants, Inc., for the U.S. Army Corps of Engineers. This study by the U.S. Geological Survey included a reassessment of the models’ calibration in more recent years—2002, 2006, 2008, and 2011—categorized respectively as low, normal, high, and extremely high flow calendar years. These years incorporated current dam-operation practices and more available data than the time period used in the original calibration. Modeled water temperatures downstream of both HCL and LOP-DEX on the MFWR were within an average of 0.68 degree Celsius (°C) of measured values; modeled temperatures downstream of Foster Dam on the South Santiam River were within an average of 0.65°C of measured values. A new CE-QUAL-W2 model was developed and calibrated for the riverine MFWR reach between Hills Creek Dam and the head of LOP, allowing an evaluation of the flow and temperature conditions in the entire MFWR system from HCL to Dexter Dam. The complex bathymetry and long residence time of HCL, combined with the relatively deep location of the power and regulating outlet structures at Hills Creek Dam, led to a HCL model that was highly sensitive to several outlet and geometric parameters related to dam structures (STR TOP, STR BOT, STR WIDTH). Release temperatures from HCL were important and often persisted downstream as they were incorporated in the MFWR model and the LOP-DEX model (downstream of MFWR). The models tended to underpredict the measured temperature of water releases from Dexter Dam during the late-September-through-December drawdown period in 2002, and again (to a lesser extent) in

  3. Physical habitat classification and instream flow modeling to determine habitat availability during low-flow periods, North Fork Shenandoah River, Virginia

    Science.gov (United States)

    Krstolic, Jennifer L.; Hayes, Donald C.; Ruhl, Peter M.

    2006-01-01

    Increasing development and increasing water withdrawals for public, industrial, and agricultural water supply threaten to reduce streamflows in the Shenandoah River basin in Virginia. Water managers need more information to balance human water-supply needs with the daily streamflows necessary for maintaining the aquatic ecosystems. To meet the need for comprehensive information on hydrology, water supply, and instream-flow requirements of the Shenandoah River basin, the U.S. Geological Survey and the Northern Shenandoah Valley Regional Commission conducted a cooperative investigation of habitat availability during low-flow periods on the North Fork Shenandoah River. Historic streamflow data and empirical data on physical habitat, river hydraulics, fish community structure, and recreation were used to develop a physical habitat simulation model. Hydraulic measurements were made during low, medium, and high flows in six reaches at a total of 36 transects that included riffles, runs, and pools, and that had a variety of substrates and cover types. Habitat suitability criteria for fish were developed from detailed fish-community sampling and microhabitat observations. Fish were grouped into four guilds of species and life stages with similar habitat requirements. Simulated habitat was considered in the context of seasonal flow regimes to show the availability of flows that sustain suitable habitat during months when precipitation and streamflow are scarce. The North Fork Shenandoah River basin was divided into three management sections for analysis purposes: the upper section, middle section, and lower section. The months of July, August, and September were chosen to represent a low-flow period in the basin with low mean monthly flows, low precipitation, high temperatures, and high water withdrawals. Exceedance flows calculated from the combined data from these three months describe low-flow periods on the North Fork Shenandoah River. Long-term records from three

  4. Mercury and selenium concentrations in biofilm, macroinvertebrates, and fish collected in the Yankee Fork of the Salmon River, Idaho, USA, and their potential effects on fish health

    Science.gov (United States)

    Rhea, Darren T.; Farag, Aïda M.; Harper, David D.; McConnell, Elizabeth; Brumbaugh, William G.

    2013-01-01

    The Yankee Fork is a large tributary of the Salmon River located in central Idaho, USA, with an extensive history of placer and dredge-mining activities. Concentrations of selenium (Se) and mercury (Hg) in various aquatic trophic levels were measured in the Yankee Fork during 2001 and 2002. Various measurements of fish health were also performed. Sites included four on the mainstem of the Yankee Fork and two off-channel sites in partially reclaimed dredge pools used as rearing habitat for cultured salmonid eggs and fry. Hg concentrations in whole mountain whitefish and shorthead sculpin ranged from 0.28 to 0.56 μg/g dry weight (dw), concentrations that are generally less than those reported to have significant impacts on fish. Biofilm and invertebrates ranged from 0.05 to 0.43 μg Hg/g dw. Se concentrations measured in biota samples from the Yankee Fork were greater than many representative samples collected in the Snake and Columbia watersheds and often exceeded literature-based toxic thresholds. Biofilm and invertebrates ranged from 0.58 to 4.66 μg Se/g dw. Whole fish ranged from 3.92 to 7.10 μg Se/g dw, and gonads ranged from 6.91 to 31.84 μg Se/g dw. Whole-body Se concentrations exceeded reported toxicological thresholds at three of four sites and concentrations in liver samples were mostly greater than concentrations shown to have negative impacts on fish health. Histological examinations performed during this study noted liver abnormalities, especially in shorthead sculpin, a bottom-dwelling species.

  5. Preliminary assessment of aggradation potential in the North Fork Stillaguamish River downstream of the State Route 530 landslide near Oso, Washington

    Science.gov (United States)

    Magirl, Christopher S.; Keith, Mackenzie K.; Anderson, Scott W.; O'Connor, Jim; Robert Aldrich,; Mastin, Mark C.

    2015-12-28

    On March 22, 2014, the State Route 530 Landslide near Oso, Washington, traveled almost 2 kilometers (km), destroyed more than 40 structures, and impounded the North Fork Stillaguamish River to a depth of 8 meters (m) and volume of 3.3×106 cubic meters (m3). The landslide killed 43 people. After overtopping and establishing a new channel through the landslide, the river incised into the landslide deposit over the course of 10 weeks draining the impoundment lake and mobilizing an estimated 280,000±56,000 m3 of predominantly sand-sized and finer sediment. During the first 4 weeks after the landslide, this eroded sediment caused downstream riverbed aggradation of 1–2 m within 1 km of the landslide and 0.4 m aggradation at Whitman Road Bridge, 3.5 km downstream. Winter high flows in 2014–15 were anticipated to mobilize an additional 220,000±44,000 m3 of sediment, potentially causing additional aggradation and exacerbating flood risk downstream of the landslide. Analysis of unit stream power and bed-material transport capacity along 35 km of the river corridor indicated that most fine-grained sediment will transport out of the North Fork Stillaguamish River, although some localized additional aggradation was possible. This new aggradation was not likely to exceed 0.1 m except in reaches within a few kilometers downstream of the landslide, where additional aggradation of up to 0.5 m is possible. Alternative river response scenarios, including continued mass wasting from the landslide scarp, major channel migration or avulsion, or the formation of large downstream wood jams, although unlikely, could result in reaches of significant local aggradation or channel change.

  6. Water temperature effects from simulated dam operations and structures in the Middle Fork Willamette River, western Oregon

    Science.gov (United States)

    Buccola, Norman L.; Turner, Daniel F.; Rounds, Stewart A.

    2016-09-14

    Significant FindingsStreamflow and water temperature in the Middle Fork Willamette River (MFWR), western Oregon, have been regulated and altered since the construction of Lookout Point, Dexter, and Hills Creek Dams in 1954 and 1961, respectively. Each year, summer releases from the dams typically are cooler than pre-dam conditions, with the reverse (warmer than pre-dam conditions) occurring in autumn. This pattern has been detrimental to habitat of endangered Upper Willamette River (UWR) Chinook salmon (Oncorhynchus tshawytscha) and UWR winter steelhead (O. mykiss) throughout multiple life stages. In this study, scenarios testing different dam-operation strategies and hypothetical dam-outlet structures were simulated using CE-QUAL-W2 hydrodynamic/temperature models of the MFWR system from Hills Creek Lake (HCR) to Lookout Point (LOP) and Dexter (DEX) Lakes to explore and understand the efficacy of potential flow and temperature mitigation options.Model scenarios were run in constructed wet, normal, and dry hydrologic calendar years, and designed to minimize the effects of Hills Creek and Lookout Point Dams on river temperature by prioritizing warmer lake surface releases in May–August and cooler, deep releases in September–December. Operational scenarios consisted of a range of modified release rate rules, relaxation of power-generation constraints, variations in the timing of refill and drawdown, and maintenance of different summer maximum lake levels at HCR and LOP. Structural scenarios included various combinations of hypothetical floating outlets near the lake surface and hypothetical new outlets at depth. Scenario results were compared to scenarios using existing operational rules that give temperature management some priority (Base), scenarios using pre-2012 operational rules that prioritized power generation over temperature management (NoBlend), and estimated temperatures from a without-dams condition (WoDams).Results of the tested model scenarios led

  7. Geohydrologic Investigations and Landscape Characteristics of Areas Contributing Water to Springs, the Current River, and Jacks Fork, Ozark National Scenic Riverways, Missouri

    Science.gov (United States)

    Mugel, Douglas N.; Richards, Joseph M.; Schumacher, John G.

    2009-01-01

    The Ozark National Scenic Riverways (ONSR) is a narrow corridor that stretches for approximately 134 miles along the Current River and Jacks Fork in southern Missouri. Most of the water flowing in the Current River and Jacks Fork is discharged to the rivers from springs within the ONSR, and most of the recharge area of these springs is outside the ONSR. This report describes geohydrologic investigations and landscape characteristics of areas contributing water to springs and the Current River and Jacks Fork in the ONSR. The potentiometric-surface map of the study area for 2000-07 shows that the groundwater divide extends beyond the surface-water divide in some places, notably along Logan Creek and the northeastern part of the study area, indicating interbasin transfer of groundwater between surface-water basins. A low hydraulic gradient occurs in much of the upland area west of the Current River associated with areas of high sinkhole density, which indicates the presence of a network of subsurface karst conduits. The results of a low base-flow seepage run indicate that most of the discharge in the Current River and Jacks Fork was from identified springs, and a smaller amount was from tributaries whose discharge probably originated as spring discharge, or from springs or diffuse groundwater discharge in the streambed. Results of a temperature profile conducted on an 85-mile reach of the Current River indicate that the lowest average temperatures were within or downstream from inflows of springs. A mass-balance on heat calculation of the discharge of Bass Rock Spring, a previously undescribed spring, resulted in an estimated discharge of 34.1 cubic feet per second (ft3/s), making it the sixth largest spring in the Current River Basin. The 13 springs in the study area for which recharge areas have been estimated accounted for 82 percent (867 ft3/s of 1,060 ft3/s) of the discharge of the Current River at Big Spring during the 2006 seepage run. Including discharge from

  8. High-resolution digital elevation model of Mount St. Helens crater and upper North Fork Toutle River basin, Washington, based on an airborne lidar survey of September 2009

    Science.gov (United States)

    Mosbrucker, Adam

    2014-01-01

    The lateral blast, debris avalanche, and lahars of the May 18th, 1980, eruption of Mount St. Helens, Washington, dramatically altered the surrounding landscape. Lava domes were extruded during the subsequent eruptive periods of 1980–1986 and 2004–2008. More than three decades after the emplacement of the 1980 debris avalanche, high sediment production persists in the North Fork Toutle River basin, which drains the northern flank of the volcano. Because this sediment increases the risk of flooding to downstream communities on the Toutle and Cowlitz Rivers, the U.S. Army Corps of Engineers (USACE), under the direction of Congress to maintain an authorized level of flood protection, built a sediment retention structure on the North Fork Toutle River in 1989 to help reduce this risk and to prevent sediment from clogging the shipping channel of the Columbia River. From September 16–20, 2009, Watershed Sciences, Inc., under contract to USACE, collected high-precision airborne lidar (light detection and ranging) data that cover 214 square kilometers (83 square miles) of Mount St. Helens and the upper North Fork Toutle River basin from the sediment retention structure to the volcano's crater. These data provide a digital dataset of the ground surface, including beneath forest cover. Such remotely sensed data can be used to develop sediment budgets and models of sediment erosion, transport, and deposition. The U.S. Geological Survey (USGS) used these lidar data to develop digital elevation models (DEMs) of the study area. DEMs are fundamental to monitoring natural hazards and studying volcanic landforms, fluvial and glacial geomorphology, and surface geology. Watershed Sciences, Inc., provided files in the LASer (LAS) format containing laser returns that had been filtered, classified, and georeferenced. The USGS produced a hydro-flattened DEM from ground-classified points at Castle, Coldwater, and Spirit Lakes. Final results averaged about five laser last

  9. Continuous water-quality monitoring and regression analysis to estimate constituent concentrations and loads in the Red River of the North at Fargo and Grand Forks, North Dakota, 2003-12

    Science.gov (United States)

    Galloway, Joel M.

    2014-01-01

    The Red River of the North (hereafter referred to as “Red River”) Basin is an important hydrologic region where water is a valuable resource for the region’s economy. Continuous water-quality monitors have been operated by the U.S. Geological Survey, in cooperation with the North Dakota Department of Health, Minnesota Pollution Control Agency, City of Fargo, City of Moorhead, City of Grand Forks, and City of East Grand Forks at the Red River at Fargo, North Dakota, from 2003 through 2012 and at Grand Forks, N.Dak., from 2007 through 2012. The purpose of the monitoring was to provide a better understanding of the water-quality dynamics of the Red River and provide a way to track changes in water quality. Regression equations were developed that can be used to estimate concentrations and loads for dissolved solids, sulfate, chloride, nitrate plus nitrite, total phosphorus, and suspended sediment using explanatory variables such as streamflow, specific conductance, and turbidity. Specific conductance was determined to be a significant explanatory variable for estimating dissolved solids concentrations at the Red River at Fargo and Grand Forks. The regression equations provided good relations between dissolved solid concentrations and specific conductance for the Red River at Fargo and at Grand Forks, with adjusted coefficients of determination of 0.99 and 0.98, respectively. Specific conductance, log-transformed streamflow, and a seasonal component were statistically significant explanatory variables for estimating sulfate in the Red River at Fargo and Grand Forks. Regression equations provided good relations between sulfate concentrations and the explanatory variables, with adjusted coefficients of determination of 0.94 and 0.89, respectively. For the Red River at Fargo and Grand Forks, specific conductance, streamflow, and a seasonal component were statistically significant explanatory variables for estimating chloride. For the Red River at Grand Forks, a time

  10. Pitch Fork

    DEFF Research Database (Denmark)

    Williams, Peter Leslie; Overholt, Daniel

    2017-01-01

    Pitch Fork is a prototype of an alternate, actuated digital musical instrument (DMI). It uses 5 infra-red and 4 piezoelectric sensors to control an additive synthesis engine. Iron bars are used as the physical point of contact in interaction with the aim of using this materials natural acoustic p...... properties as a control signal for aspects of the digitally produced sound. This choice of material was also chosen to affect player experience. Sensor readings are relayed to a Macbook via an Arduino Mega. Mappings and audio output signal is carried out with Pure Data Extended....

  11. Selected trace-element and synthetic-organic compound data for streambed sediment from the Clark Fork-Pend Oreille and Spokane River basins, Montana, Idaho, and Washington, 1998

    Science.gov (United States)

    Beckwith, Michael A.

    2002-01-01

    Streambed-sediment samples were collected at 22 sites during the summer of 1998 as part of the U.S. Geological Survey National Water-Quality Assessment Program. Sampling sites in the Clark Fork-Pend Oreille and Spokane River basins represented a wide range of environmental conditions including pristine mountain streams and large rivers affected by mining-related and urban activities. Samples were analyzed for 45 inorganic major and trace elements, 109 syn­thetic organic compounds, and carbon. This report pre­sents the selected results of streambed-sediment sampling from the Clark Fork-Pend Oreille and Spo­kane River basins in Montana, Idaho, and Washington.

  12. Do Knickpoints Unzip Watersheds? Longitudinal Observations of Terrace and Hillslope Response to Mainstem Incision along the South Fork Eel River, California

    Science.gov (United States)

    Wehrs, K.; Crosby, B. T.

    2017-12-01

    River response to changes in climate and relative base level often leave behind a legacy of transient landforms that enable the interpretation of past events. The dominant paradigm is that base level fall initiates a wave of mainstem incision that progressively transmits change upstream. Mainstem-adjacent hillslopes coupled to the channel subsequently respond as their toe slopes are steepened. To test this paradigm, we first use a longitudinal set of mainstem terrace ages to evaluate whether incision incrementally progresses upstream or is contemporaneous. Second, we explore longitudinal variations in mainstem-adjacent mass movements to evaluate whether they reflect a time and space progression in response. The South Fork Eel River in northern California contains over 600 mainstem-adjacent mass movements and 60 m tall, longitudinally extensive strath terraces that record a landscape response to river incision. We use Optically Stimulated Luminescence, with feldspars and coarse-grained sampling technique, to determine the depositional age of alluvial fill atop the strath terrace. If terrace abandonment progressively young upstream, this suggests that base level fall was not spatially contemporaneous, but rather time progressive. As a consequence, the age, form, and extent of mass wasting events should also vary longitudinally. Because terraces isolate hillslopes from the base level fall signal, we use these surfaces to quantify hillslope form and function independent of that forcing. Preliminary results using mainstem-parallel, 1 m LiDAR, show significant variation in size of mass movements throughout the basin, with planar, linearly moving translational landslides dominating throughout the catchment. In the lower basin, well downstream of the current knickzone, we see an increase in mass movement concentration, reactivation, and overall extent of mass movements. Multiple factors confound our interpretation of hillslope morphology and response, due to changes in

  13. Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007

    Science.gov (United States)

    Alvarez, David A.; Cranor, Walter L.; Perkins, Stephanie D.; Schroeder, Vickie L.; Werner, Stephen; Furlong, Edward T.; Holmes, John

    2008-01-01

    Declining fish health, fish exhibiting external lesions, incidences of intersex, and death, have been observed recently within the Potomac River basin. The basin receives surface runoff and direct inputs from agricultural, industrial, and other human activities. Two locations on the North Fork of the Shenandoah River were selected for study in an attempt to identify chemicals that may have contributed to the declining fish health. Two passive sampling devices, semipermeable membrane devices (SPMDs) and polar organic chemical integrative samplers (POCIS), were deployed during consecutive two-month periods during the spring and early summer of 2007 to measure select organic contaminants to which fish may have been exposed. This study determined that concentrations of persistent hydrophobic contaminants, such as polycyclic aromatic hydrocarbons (wastewater treatment plant effluent or septic tank discharges were identified. In contrast, para-cresol, N,N-diethyltoluamide, and caffeine commonly were detected. Prescription pharmaceuticals including carbamazepine, venlafaxine, and 17a-ethynylestradiol were at low concentrations. Extracts from the passive samplers also were screened for the presence of estrogenic chemicals using the yeast estrogen screen. An estrogenic response was observed in POCIS samples from both sites, whereas SPMD samples exhibited little to no estrogenicity. This indicates that the chemicals producing the estrogenic response have a greater water solubility and are, therefore, less likely to bioaccumulate in fatty tissues of organisms.

  14. Flood-inundation maps for a 9.1-mile reach of the Coast Fork Willamette River near Creswell and Goshen, Lane County, Oregon

    Science.gov (United States)

    Hess, Glen W.; Haluska, Tana L.

    2016-04-13

    Digital flood-inundation maps for a 9.1-mile reach of the Coast Fork Willamette River near Creswell and Goshen, Oregon, were developed by the U.S. Geological Survey (USGS) in cooperation with the U.S. Army Corps of Engineers (USACE). The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/, depict estimates of the areal extent and depth of flooding corresponding to selected stages at the USGS streamgage at Coast Fork Willamette River near Goshen, Oregon (14157500), at State Highway 58. Current stage at the streamgage for estimating near-real-time areas of inundation may be obtained at http://waterdata.usgs.gov/or/nwis/uv/?site_no=14157500&PARAmeter_cd=00065,00060. In addition, the National Weather Service (NWS) forecasted peak-stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation.In this study, areas of inundation were provided by USACE. The inundated areas were developed from flood profiles simulated by a one-dimensional unsteady step‑backwater hydraulic model. The profiles were checked by the USACE using documented high-water marks from a January 2006 flood. The model was compared and quality assured using several other methods. The hydraulic model was then used to determine eight water-surface profiles at various flood stages referenced to the streamgage datum and ranging from 11.8 to 19.8 ft, approximately 2.6 ft above the highest recorded stage at the streamgage (17.17 ft) since 1950. The intervals between stages are variable and based on annual exceedance probability discharges, some of which approximate NWS action stages.The areas of inundation and water depth grids provided to USGS by USACE were used to create interactive flood‑inundation maps. The availability of these maps with current stage from USGS streamgage and forecasted stream stages from the NWS provide emergency management

  15. An aerial radiological survey of the Oyster Creek Nuclear Power Plant and surrounding area, Forked River, New Jersey. Date of survey: September 18--25, 1992

    International Nuclear Information System (INIS)

    Hopkins, H.A.; McCall, K.A.

    1994-05-01

    An aerial radiological survey was conducted over the Oyster Creek Nuclear Power Plant in Forked River, New Jersey, during the period September 18 through September 24, 1992. The survey was conducted at an altitude of 150 feet (46 meters) over a 26-square-mile (67-square-kilometer) area centered on the power station. The purpose of the survey was to document the terrestrial gamma radiation environment of the Oyster Creek Nuclear Power plant and surrounding area. The results of the aerial survey are reported as inferred gamma radiation exposure rates at 1 meter above ground level in the form of a contour map. Outside the plant boundary, exposure rates were found to vary between 4 and 10 microroentgens per hour and were attributed to naturally-occurring uranium, thorium, and radioactive potassium gamma emitters. The aerial data were compared to ground-based benchmark exposure rate measurements and radionuclide assays of soil samples obtained within the survey boundary. The ground-based measurements were found to be in good agreement with those inferred from the aerial measuring system. A previous survey of the power plant was conducted in August 1969 during its initial startup phase. Exposure rates and radioactive isotopes revealed in both surveys were consistent and within normal terrestrial background levels

  16. A physiological approach to quantifying thermal habitat quality for redband rainbow trout (Oncorhynchus mykiss gairdneri) in the south Fork John Day River, Oregon

    Science.gov (United States)

    Feldhaus, J.W.; Heppell, S.A.; Li, H.; Mesa, M.G.

    2010-01-01

    We examined tissue-specific levels of heat shock protein 70 (hsp70) and whole body lipid levels in juvenile redband trout (Oncorhynchus mykiss gairdneri) from the South Fork of the John Day River (SFJD), Oregon, with the goal of determining if these measures could be used as physiological indicators of thermal habitat quality for juvenile redband trout. Our objectives were to determine the hsp70 induction temperature in liver, fin, and white muscle tissue and characterize the relation between whole body lipids and hsp70 for fish in the SFJD. We found significant increases in hsp70 levels between 19 and 22??C in fin, liver, and white muscle tissue. Maximum hsp70 levels in liver, fin, and white muscle tissue occurred when mean weekly maximum temperatures (MWMT) exceeded 20-22??C. In general, the estimated hsp70 induction temperature for fin and white muscle tissue was higher than liver tissue. Whole body lipid levels began to decrease when MWMT exceeded 20. 4??C. There was a significant interaction between temperature and hsp70 in fin and white muscle tissue, but not liver tissue. Collectively, these results suggest that increased hsp70 levels in juvenile redband trout are symptomatic of thermal stress, and that energy storage capacity decreases with this stress. The possible decrease in growth potential and fitness for thermally stressed individuals emphasizes the physiological justification for thermal management criteria in salmon-bearing streams. ?? Springer Science+Business Media B.V. 2010.

  17. Hyporheic Exchange Flows and Biogeochemical Patterns near a Meandering Stream: East Fork of the Jemez River, Valles Caldera National Preserve, New Mexico

    Science.gov (United States)

    Christensen, H.; Wooten, J. P.; Swanson, E.; Senison, J. J.; Myers, K. D.; Befus, K. M.; Warden, J.; Zamora, P. B.; Gomez, J. D.; Wilson, J. L.; Groffman, A.; Rearick, M. S.; Cardenas, M. B.

    2012-12-01

    A study by the 2012 Hydrogeology Field Methods class of the University of Texas at Austin implemented multiple approaches to evaluate and characterize local hyporheic zone flow and biogeochemical trends in a highly meandering reach of the of the East Fork of the Jemez River, a fourth order stream in northwestern New Mexico. This section of the Jemez River is strongly meandering and exhibits distinct riffle-pool morphology. The high stream sinuosity creates inter-meander hyporheic flow that is also largely influenced by local groundwater gradients. In this study, dozens of piezometers were used to map the water table and flow vectors were then calculated. Surface water and ground water samples were collected and preserved for later geochemical analysis by ICPMS and HPLC, and unstable parameters and alkalinity were measured on-site. Additionally, information was collected from thermal monitoring of the streambed, stream gauging, and from a series of electrical resistivity surveys forming a network across the site. Hyporheic flow paths are suggested by alternating gaining and losing sections of the stream as determined by stream gauging at multiple locations along the reach. Water table maps and calculated fluxes across the sediment-water interface also indicate hyporheic flow paths. We find variability in the distribution of biogeochemical constituents (oxidation-reduction potential, nitrate, ammonium, and phosphate) along interpreted flow paths which is partly consistent with hyporheic exchange. The variability and heterogeneity of reducing and oxidizing conditions is interpreted to be a result of groundwater-surface water interaction. Two-dimensional mapping of biogeochemical parameters show redox transitions along interpreted flow paths. Further analysis of various measured unstable chemical parameters results in observable trends strongly delineated along these preferential flow paths that are consistent with the direction of groundwater flow and the assumed

  18. Trophic feasibility of reintroducing anadromous salmonids in three reservoirs on the north fork Lewis River, Washington: Prey supply and consumption demand of resident fishes

    Science.gov (United States)

    Sorel, Mark H.; Hansen, Adam G.; Connelly, Kristin A.; Beauchamp, David A.

    2016-01-01

    The reintroduction of anadromous salmonids in reservoirs is being proposed with increasing frequency, requiring baseline studies to evaluate feasibility and estimate the capacity of reservoir food webs to support reintroduced populations. Using three reservoirs on the north fork Lewis River as a case study, we demonstrate a method to determine juvenile salmonid smolt rearing capacities for lakes and reservoirs. To determine if the Lewis River reservoirs can support reintroduced populations of juvenile stream-type Chinook Salmon Oncorhynchus tshawytscha, we evaluated the monthly production of daphniaDaphnia spp. (the primary zooplankton consumed by resident salmonids in the system) and used bioenergetics to model the consumption demand of resident fishes in each reservoir. To estimate the surplus of Daphnia prey available for reintroduced salmonids, we assumed a maximum sustainable exploitation rate and accounted for the consumption demand of resident fishes. The number of smolts that could have been supported was estimated by dividing any surplus Daphnia production by the simulated consumption demand of an individual Chinook Salmon fry rearing in the reservoir to successful smolt size. In all three reservoirs, densities of Daphnia were highest in the epilimnion, but warm epilimnetic temperatures and the vertical distribution of planktivores suggested that access to abundant epilimnetic prey was limited. By comparing accessible prey supply and demand on a monthly basis, we were able to identify potential prey supply bottlenecks that could limit smolt production and growth. These results demonstrate that a bioenergetics approach can be a valuable method of examining constraints on lake and reservoir rearing capacity, such as thermal structure and temporal food supply. This method enables numerical estimation of rearing capacity, which is a useful metric for managers evaluating the feasibility of reintroducing Pacific salmon Oncorhynchus spp. in lentic systems.

  19. Evaluation of the behavior and movement patterns of adult coho salmon and steelhead in the North Fork Toutle River, Washington, 2005-2009

    Science.gov (United States)

    Liedtke, Theresa L.; Kock, Tobias J.; Rondorf, Dennis W.

    2013-01-01

    The 1980 eruption of Mount St. Helens severely affected the North Fork Toutle River (hereafter Toutle River), Washington, and threatened anadromous salmon (Oncorhynchus spp.) populations in the basin. The Toutle River was further affected in 1989 when a sediment retention structure (SRS) was constructed to trap sediments in the upper basin. The SRS completely blocked upstream volitional passage, so a fish collection facility (FCF) was constructed to trap adult coho salmon (O. kisutch) and steelhead (O. mykiss) so they could be transported upstream of the SRS. The Washington Department of Fish and Wildlife (WDFW) has operated a trap-and-haul program since 1989 to transport coho salmon and steelhead into tributaries of the Toutle River, upstream of the SRS. Although this program has allowed wild coho salmon and steelhead populations to persist in the Toutle River basin, the trap-andhaul program has faced many challenges that may be limiting the effectiveness of the program. We conducted a multi-year evaluation during 2005–2009 to monitor tagged fish in the upper Toutle River to provide information on the movements and behavior of adult coho salmon and steelhead, and to evaluate the efficacy of the FCF. Radio-tagged coho salmon and steelhead were released: (1) in Toutle River tributaries to evaluate the behavior and movements of fish released as part of the trap-and-haul program; (2) between the FCF and SRS to determine if volitional upstream passage through the SRS spillway was possible; (3) in the sediment plain upstream of the SRS to determine if volitional passage through the sediment plain was possible; and (4) downstream of the FCF to evaluate the efficacy of the structure. We also deployed an acoustic camera in the FCF to monitor fish movements near the entrance to the FCF, and in the fish holding vault where coho salmon and steelhead are trapped. A total of 20 radio-tagged coho salmon and 10 radio-tagged steelhead were released into Alder and Hoffstadt

  20. Changes in streamflow and summary of major-ion chemistry and loads in the North Fork Red River basin upstream from Lake Altus, northwestern Texas and western Oklahoma, 1945-1999

    Science.gov (United States)

    Smith, S. Jerrod; Wahl, Kenneth L.

    2003-01-01

    Upstream from Lake Altus, the North Fork Red River drains an area of 2,515 square miles. The quantity and quality of surface water are major concerns at Lake Altus, and water-resource managers and consumers need historical information to make informed decisions about future development. The Lugert-Altus Irrigation District relies on withdrawals from the lake to sustain nearly 46,000 acres of agricultural land. Kendall's tau tests of precipitation data indicated no statistically significant trend over the entire 100 years of available record. However, a significant increase in precipitation occurred in the last 51 years. Four streamflow-gaging stations with more than 10 years of record were maintained in the basin. These stations recorded no significant trends in annual streamflow volume. Two stations, however, had significant increasing trends in the base-flow index, and three had significant decreasing trends in annual peak flows. Major-ion chemistry in the North Fork Red River is closely related to the chemical composition of the underlying bedrock. Two main lithologies are represented in the basin upstream from Lake Altus. In the upper reaches, young and poorly consolidated sediments include a range of sizes from coarse gravel to silt and clay. Nearsurface horizons commonly are cemented as calcium carbonate caliche. Finer-grained gypsiferous sandstones and shales dominate the lower reaches of the basin. A distinct increase in dissolved solids, specifically sodium, chloride, calcium, and sulfate, occurs as the river flows over rocks that contain substantial quantities of gypsum, anhydrite, and dolomite. These natural salts are the major dissolved constituents in the North Fork Red River.

  1. An Inventory and Evaluation of Architectural and Engineering Resources of the Big South Fork National River and Recreation Area, Tennessee and Kentucky.

    Science.gov (United States)

    1982-02-25

    coordinated multidisciplinary study of both the architectural and engineering resources of the National Area. Both research b1 orientation and...South Fork just north of Rugby , and traveled through the site where Jamestown, Tennessee, now stands. A third trail, the Chickamauga Path, left the...Thomas Hughes (1881), the founder of the English colony of Rugby , Tennessee, described his neighbors in the Big South Fork area as mostly poor men

  2. Effects of the Upper Taum Sauk Reservoir Embankment Breach on the Surface-Water Quality and Sediments of the East Fork Black River and the Black River, Southeastern Missouri - 2006-07

    Science.gov (United States)

    Barr, Miya N.

    2009-01-01

    On December 14, 2005, a 680-foot wide section of the upper reservoir embankment of the Taum Sauk pump-storage hydroelectric powerplant located in Reynolds County, Missouri, suddenly failed. This catastrophic event sent approximately 1.5 billion gallons of water into the Johnson's Shut-Ins State Park and into the East Fork Black River, and deposited enormous quantities of rock, soil, and vegetation in the flooded areas. Water-quality data were collected within and below the impacted area to study and document the changes to the riverene system. Data collection included routine, event-based, and continuous surface-water quality monitoring as well as suspended- and streambed-sediment sampling. Surface water-quality samples were collected and analyzed for a suite of physical and chemical constituents including: turbidity; nutrients; major ions such as calcium, magnesium, and potassium; total suspended solids; total dissolved solids; trace metals such as aluminum, iron, and lead; and suspended-sediment concentrations. Suspended-sediment concentrations were used to calculate daily sediment discharge. A peculiar blue-green coloration on the water surface of the East Fork Black River and Black River was evident downstream from the lower reservoir during the first year of the study. It is possible that this phenomenon was the result of 'rock flour' occurring when the upper reservoir embankment was breached, scouring the mountainside and producing extremely fine sediment particles, or from the alum-based flocculent used to reduce turbidity in the lower reservoir. It also was determined that no long-term effects of the reservoir embankment breach are expected as the turbidity and concentrations of trace metals such as total recoverable aluminum, dissolved aluminum, dissolved iron, and suspended-sediment concentration graphically decreased over time. Larger concentrations of these constituents during the beginning of the study also could be a direct result of the alum

  3. Model Watershed Plan; Lemhi, Pahsimeroi, and East Fork of the Salmon River Management Plan, 1995 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Swift, Ralph

    1995-11-01

    Idaho`s Model Watershed Project was established as part of the Northwest Power Planning Council`s plan for salmon recovery in the Columbia River Basin. The Council`s charge was simply stated and came without strings. The tasks were to identify actions within the watershed that are planned or needed for salmon habitat, and establish a procedure for implementing habitat-improvement measures. The Council gave the responsibility of developing this project to the Idaho Soil Conservation Commission. This Model Watershed Plan is intended to be a dynamic plan that helps address these two tasks. It is not intended to be the final say on either. It is also not meant to establish laws, policies, or regulations for the agencies, groups, or individuals who participated in the plan development.

  4. Distribution of metals during digestion by cutthroat trout fed benthic invertebrates contaminated in the Clark Fork River, Montana and the Coeur d'Alene River, Idaho, U.S.A., and fed artificially contaminated Artemia

    Science.gov (United States)

    Farag, A.M.; Suedkamp, M.J.; Meyer, J.S.; Barrows, R.; Woodward, D.F.

    2000-01-01

    The concentrations of essential amino acids in three, undigested invertebrate diets collected from the Clark Fork River (CFR) for cutthroat trout were similar to each other, but were c. 25–75% less than Artemia that were exposed to a mixture of arsenic, copper, cadmium, lead and zinc in the laboratory. The Artemia diet appeared less palatable and the texture, quantity and appearance of the intestinal contents differed between fish fed the Artemia and CFR diets. The Pb% in the fluid fraction of the intestinal contents was greater for the Artemia (29%) than for the CFR diets (10–17%), and the Cu% in the amino acid plus metal fraction of the intestinal contents was greater for the Artemia (78%) than for two of the three CFR diets (67% and 70%). Intestinal contents of fish fed invertebrate diets collected from various sites on the Coeur d'Alene River (CDA), Idaho, were similar in texture, quantity, and appearance. For fish fed the CDA diets, differences in the distribution of metals among fractions of the digestive fluids appeared to be related to concentrations of metals in the invertebrate diets. Pb% was lowest of all metals in the fluid portion of the intestinal contents. However, >80% of all metals in the hind gut were associated with the particulate fraction where they may still be available for uptake through pinocytosis.

  5. Characterization of salinity loads and selenium loads in the Smith Fork Creek region of the Lower Gunnison River Basin, western Colorado, 2008-2009

    Science.gov (United States)

    Richards, Rodney J.; Linard, Joshua I.; Hobza, Christopher M.

    2014-01-01

    The lower Gunnison River Basin of the Colorado River Basin has elevated salinity and selenium levels. The Colorado River Basin Salinity Control Act of June 24, 1974 (Public Law 93–320, amended by Public Law 98–569), authorized investigation of the Lower Gunnison Basin Unit Salinity Control Project by the U.S. Department of the Interior. The Bureau of Reclamation (Reclamation) and the Natural Resources Conservation Service are responsible for assessing and implementing measures to reduce salinity and selenium loading in the Colorado River Basin. Cost-sharing programs help farmers, ranchers, and canal companies improve the efficiency of water delivery systems and irrigation practices. The delivery systems (irrigation canals) have been identified as potential sources of seepage, which can contribute to salinity loading. Reclamation wants to identify seepage from irrigation systems in order to maximize the effectiveness of the various salinity-control methods, such as polyacrylamide lining and piping of irrigation canals programs. The U.S. Geological Survey, in cooperation with Reclamation, developed a study to characterize the salinity and selenium loading of seven subbasins in the Smith Fork Creek region and identify where control efforts can be maximized to reduce salinity and selenium loading. Total salinity loads ranged from 27.9±19.1 tons per year (t/yr) to 87,500±80,500 t/yr. The four natural subbasins—BkKm, RCG1, RCG2, and SF1—had total salinity loads of 27.9±19.1 t/yr, 371±248 t/yr, 2,180±1,590 t/yr, and 4,200±2,720 t/yr, respectively. The agriculturally influenced sites had salinity loads that ranged from 7,580±6,900 t/yr to 87,500±80,500 t/yr. Salinity loads for the subbasins AL1, B1, CK1, SF2, and SF3 were 7,580±6,900 t/yr; 28,300±26,700 t/yr; 48,700±36,100 t/yr; 87,500±80,900 t/yr; and 52,200±31,800 t/yr, respectively. The agricultural salinity load was separated into three components: tail water, deep percolation, and canal seepage

  6. Field Comparisons of the Elwha Bedload Sampler and an Acoustic Gravel-transport Sensor: Middle Fork of the Piedra River, Colorado, USA

    Science.gov (United States)

    Downing, J.; Ryan, S. E.

    2001-12-01

    Ten simultaneous bedload measurements were made with an Elhwa sampler and an acoustic-gravel-transport sensor (GTS) on the Middle Fork of the Piedra River in southwestern Colorado near the end of the spring freshet in water year 2001. The purpose was to compare bedload samples with acoustic measurements acquired under field conditions. Upstream of the measurement site, the river drains 86 km2 of andesite, ash flows, tuffs, and breccias in the San Juan Mountains, contributing a relatively high sediment load to the river system. The channel transitions from step-pools at high elevations to a plane bed with a slope of 0.018 in the study reach. Channel width, mean depth and bank-full velocity at the site are: 13.6 m, 0.52, and 1.5 m s-1. The D50 of the riverbed surface is 0.08 m which is 6 to 40 times larger than the D50s of the bedload samples. D16 and D84 of the bed = 0.02 and 0.21 m respectively. Water discharges from 7.3 to 9.3 m3 s-1 transported about 0.01 kg of gravel m-1 s-1 in the channel. Transport of coarse gravel (8-64 mm) ranged from 0.00063 to 0.024 kg m-1 s-1. The Elwha sampler is a portable, pressure-differential trap with a 0.2 m wide by 0.1 m high opening. The acoustic sensor is a 0.025-m wide by 0.1 m high strip of PVDF piezoelectric film connected to a signal processor and bonded to an aluminum pressure plate. When the plate is struck by stones, the GTS produces signal peaks with areas that are accurate measures of stone momentum. The GTS was calibrated with steels balls dropped on the pressure plate in still water to develop a curve of ball momentum as a function of peak areas. Based on these calibrations, the standard error of the GTS momentum estimates is 0.0017 kg m s-1. Five transects with 30 verticals, each occupied for 60 s, were completed with the sampler and GTS separated by < 1 m. Five additional verticals were occupied for about 1800 s each with the instruments separated by < 0.5 m. The trapped material was sieved and weighed and the water

  7. Evaluation of LiDAR-acquired bathymetric and topographic data accuracy in various hydrogeomorphic settings in the Deadwood and South Fork Boise Rivers, West-Central Idaho, 2007

    Science.gov (United States)

    Skinner, Kenneth D.

    2011-01-01

    High-quality elevation data in riverine environments are important for fisheries management applications and the accuracy of such data needs to be determined for its proper application. The Experimental Advanced Airborne Research LiDAR (Light Detection and Ranging)-or EAARL-system was used to obtain topographic and bathymetric data along the Deadwood and South Fork Boise Rivers in west-central Idaho. The EAARL data were post-processed into bare earth and bathymetric raster and point datasets. Concurrently with the EAARL surveys, real-time kinematic global positioning system surveys were made in three areas along each of the rivers to assess the accuracy of the EAARL elevation data in different hydrogeomorphic settings. The accuracies of the EAARL-derived raster elevation values, determined in open, flat terrain, to provide an optimal vertical comparison surface, had root mean square errors ranging from 0.134 to 0.347 m. Accuracies in the elevation values for the stream hydrogeomorphic settings had root mean square errors ranging from 0.251 to 0.782 m. The greater root mean square errors for the latter data are the result of complex hydrogeomorphic environments within the streams, such as submerged aquatic macrophytes and air bubble entrainment; and those along the banks, such as boulders, woody debris, and steep slopes. These complex environments reduce the accuracy of EAARL bathymetric and topographic measurements. Steep banks emphasize the horizontal location discrepancies between the EAARL and ground-survey data and may not be good representations of vertical accuracy. The EAARL point to ground-survey comparisons produced results with slightly higher but similar root mean square errors than those for the EAARL raster to ground-survey comparisons, emphasizing the minimized horizontal offset by using interpolated values from the raster dataset at the exact location of the ground-survey point as opposed to an actual EAARL point within a 1-meter distance. The

  8. Eukaryotic DNA Replication Fork.

    Science.gov (United States)

    Burgers, Peter M J; Kunkel, Thomas A

    2017-06-20

    This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.

  9. Summary of surface-water-quality data collected for the Northern Rockies Intermontane Basins National Water-Quality Assessment Program in the Clark Fork-Pend Oreille and Spokane River basins, Montana, Idaho, and Washington, water years 1999-2001

    Science.gov (United States)

    Beckwith, Michael A.

    2003-01-01

    Water-quality samples were collected at 10 sites in the Clark Fork-Pend Oreille and Spokane River Basins in water years 1999 – 2001 as part of the Northern Rockies Intermontane Basins (NROK) National Water-Quality Assessment (NAWQA) Program. Sampling sites were located in varied environments ranging from small streams and rivers in forested, mountainous headwater areas to large rivers draining diverse landscapes. Two sampling sites were located immediately downstream from the large lakes; five sites were located downstream from large-scale historical mining and oreprocessing areas, which are now the two largest “Superfund” (environmental remediation) sites in the Nation. Samples were collected during a wide range of streamflow conditions, more frequently during increasing and high streamflow and less frequently during receding and base-flow conditions. Sample analyses emphasized major ions, nutrients, and selected trace elements. Streamflow during the study ranged from more than 130 percent of the long-term average in 1999 at some sites to 40 percent of the long-term average in 2001. River and stream water in the study area exhibited small values for specific conductance, hardness, alkalinity, and dissolved solids. Dissolved oxygen concentrations in almost all samples were near saturation. Median total nitrogen and total phosphorus concentrations in samples from most sites were smaller than median concentrations reported for many national programs and other NAWQA Program study areas. The only exceptions were two sites downstream from large wastewater-treatment facilities, where median concentrations of total nitrogen exceeded the national median. Maximum concentrations of total phosphorus in samples from six sites exceeded the 0.1 milligram per liter threshold recommended for limiting nuisance aquatic growth. Concentrations of arsenic, cadmium, copper, lead, mercury, and zinc were largest in samples from sites downstream from historical mining and ore

  10. Economic fork lifters

    Energy Technology Data Exchange (ETDEWEB)

    Roedig, W

    1981-01-01

    Increased energy costs attribute new interest to the choice between electric fork lifters and fork lifters driven by combustion engines. The advantages and shortcomings of the two drive systems are listed. As previous cost-comparisons are no longer up-to-date a new comparison is made in order to find out which of the three types works most economically: electric diesel- or fuel gas driven fork lifters. The comparison is based on a one-year-operation with a load capacity of 1,5 to 5 tons under normal stress conditions. The following parameters are compared: sum of investments, depreciation, interest rates, fixed costs per annum and per hour of operation. Variable costs like: repair costs, costs for replacement parts, energy cost and total cost. The three-wheeled electric fork lifter has proved to be the most economic one followed by the diesel-driven fork lifter, the four-wheel electric fork lifter and the fuel-gas driven fork lifter.

  11. The role of episodic fire-related debris flows on long-term (103-104) sediment yields in the Middle Fork Salmon River Watershed, in central Idaho

    Science.gov (United States)

    Riley, K. E.; Pierce, J. L.; Hopkins, A.

    2010-12-01

    Episodic fire-related debris flows contribute large amounts of sediment and large woody debris to streams. This study evaluates fire-related sedimentation from small steep tributaries of the Middle Fork Salmon River (MFSR) in central Idaho to evaluate the timing, frequency, and magnitude of episodic fire-related sedimentation on long-term (10 3-10 4) sediment yields. The MFSR lies within the Northern Rocky Mountains and encompasses a range of ecosystems including high elevation (~3,000 -1,700 m) subalpine pine and spruce forests, mid-elevation (2650 - 1130 m) montane Douglas-fir and ponderosa pine-dominated forests and low elevation (~ 1,800 - 900 m) sagebrush steppe. Recent debris flow events in tributaries of the MFSR appear to primarily result from increased surface runoff, rilling, and progressive sediment bulking following high severity fires. This study estimates: 1) the volume of sediment delivered by four recent (1997-2008) fire-related debris flow events using real time kinematic GPS surveys, and 2) the timing of Holocene fire-related debris flow events determined by 14C dating charcoal fragments preserved in buried burned soils and within fire-related deposits. Our measured volumes of the four recent debris flow events are compared to two empirically derived volume estimates based on remotely sensed spatial data (burn severity and slope), measured geometric data (longitudinal profile, cross sectional area, flow banking angle), and precipitation records. Preliminary stratigraphic profiles in incised alluvial fans suggest that a large percentage of alluvial fan thickness is composed of fire-related deposits suggesting fire-related hillslope erosion is a major process delivering sediment to alluvial fans and to the MFSR. Fire-related deposits from upper basins compose ~71% of total alluvial fan thickness, while fire-related deposits from lower basins make up 36% of alluvial fan thickness. However, lower basins are less densely vegetated with small diameter

  12. Fate and transport of petroleum hydrocarbons in soil and ground water at Big South Fork National River and Recreation Area, Tennessee and Kentucky, 2002-2003

    Science.gov (United States)

    Williams, Shannon D.; Ladd, David E.; Farmer, James

    2006-01-01

    In 2002 and 2003, the U.S. Geological Survey (USGS), by agreement with the National Park Service (NPS), investigated the effects of oil and gas production operations on ground-water quality at Big South Fork National River and Recreation Area (BISO) with particular emphasis on the fate and transport of petroleum hydrocarbons in soils and ground water. During a reconnaissance of ground-water-quality conditions, samples were collected from 24 different locations (17 springs, 5 water-supply wells, 1 small stream, and 1 spring-fed pond) in and near BISO. Benzene, toluene, ethylbenzene, and xylene (BTEX) compounds were not detected in any of the water samples, indicating that no widespread contamination of ground-water resources by dissolved petroleum hydrocarbons probably exists at BISO. Additional water-quality samples were collected from three springs and two wells for more detailed analyses to obtain additional information on ambient water-quality conditions at BISO. Soil gas, soil, water, and crude oil samples were collected at three study sites in or near BISO where crude oil had been spilled or released (before 1993). Diesel range organics (DRO) were detected in soil samples from all three of the sites at concentrations greater than 2,000 milligrams per kilogram. Low concentrations (less than 10 micrograms per kilogram) of BTEX compounds were detected in lab-analyzed soil samples from two of the sites. Hydrocarbon-degrading bacteria counts in soil samples from the most contaminated areas of the sites were not greater than counts for soil samples from uncontaminated (background) sites. The elevated DRO concentrations, the presence of BTEX compounds, and the low number of -hydrocarbon-degrading bacteria in contaminated soils indicate that biodegradation of petroleum hydrocarbons in soils at these sites is incomplete. Water samples collected from the three study sites were analyzed for BTEX and DRO. Ground-water samples were collected from three small springs at the

  13. SC tuning fork

    CERN Document Server

    The tuning fork used to modulate the radiofrequency system of the synchro cyclotron (SC) from 1957 to 1973. This piece is an unused spare part. The SC was the 1st accelerator built at CERN. It operated from August 1957 until it was closed down at the end of 1990. In the SC the magnetic field did not change with time, and the particles were accelerated in successive pulses by a radiofrequency voltage of some 20kV which varied in frequency as they spiraled outwards towards the extraction radius. The frequency varied from 30MHz to about 17Mz in each pulse. The tuning fork vibrated at 55MHz in vacuum in an enclosure which formed a variable capacitor in the tuning circuit of the RF system, allowing the RF to vary over the appropriate range to accelerate protons from the centre of the macine up to 600Mev at extraction radius. In operation the tips of the tuning fork blade had an amplitude of movement of over 1 cm. The SC accelerator underwent extensive improvements from 1973 to 1975, including the installation of a...

  14. Aerial gamma ray and magnetic survey: Minnesota Project, Thief River Falls, Grand Forks, Fargo, Milbank, Watertown, New Ulm and St. Cloud quadrangles of North Dakota, South Dakota and Minnesota. Final report

    International Nuclear Information System (INIS)

    1979-10-01

    During the months of August and September 1979, geoMetrics, Inc., collected 12,415 line miles of high sensitivity airborne radiometric and magnetic data in adjoining portions of South Dakota and Minnesota over seven 1 by 2 degree NTMS quadrangles (Thief River Falls, Grand Forks, Fargo, Milbank, Watertown, New Ulm, and St. Cloud) as part of the Department of Energy's National Uranium Resource Evaluation Program. All radiometric and magnetic data were fully corrected and interpreted by geoMetrics and are presented as eight volumes (one Volume I and seven Volume II's). Regional geology for these seven quadrangles can be divided into two logical sections. The first comprises the surficial glacial deposits, which mantle most of the area and can be up to hundreds of feet thick. The second section consists of the underlying bedrock which is exposed in small scattered outcrops, generally along major drainages. No sedimentary structures exist within the quadrangles. As of this writing, no known uranium deposits exist within the seven quadrangles

  15. Cultural Resource Inventory of Lands in and Adjacent to the City of Rochester, Minnesota Flood Control Project on the South Fork Zumbro River,

    Science.gov (United States)

    1981-09-14

    the area directly east of present- day Rochester contained fire-maintained oak openings and barrens . This area consisted of oak groves or single trees...Archaeological Survey of Lands Adjacent to the Pine River Reservoir; University of Minnesota and St. Paul District Corps of Engineers; Archaeological Field...Archaeological Field Services, Inc.; Principal Investigator. 1980 An Archaeological Reconnaissance Survey of the Harrison Hills Project, Plymouth , Hennepin

  16. Continuous real-time water-quality monitoring and regression analysis to compute constituent concentrations and loads in the North Fork Ninnescah River upstream from Cheney Reservoir, south-central Kansas, 1999–2012

    Science.gov (United States)

    Stone, Mandy L.; Graham, Jennifer L.; Gatotho, Jackline W.

    2013-01-01

    Cheney Reservoir, located in south-central Kansas, is the primary water supply for the city of Wichita. The U.S. Geological Survey has operated a continuous real-time water-quality monitoring station since 1998 on the North Fork Ninnescah River, the main source of inflow to Cheney Reservoir. Continuously measured water-quality physical properties include streamflow, specific conductance, pH, water temperature, dissolved oxygen, and turbidity. Discrete water-quality samples were collected during 1999 through 2009 and analyzed for sediment, nutrients, bacteria, and other water-quality constituents. Regression models were developed to establish relations between discretely sampled constituent concentrations and continuously measured physical properties to compute concentrations of those constituents of interest that are not easily measured in real time because of limitations in sensor technology and fiscal constraints. Regression models were published in 2006 that were based on data collected during 1997 through 2003. This report updates those models using discrete and continuous data collected during January 1999 through December 2009. Models also were developed for four new constituents, including additional nutrient species and indicator bacteria. In addition, a conversion factor of 0.68 was established to convert the Yellow Springs Instruments (YSI) model 6026 turbidity sensor measurements to the newer YSI model 6136 sensor at the North Ninnescah River upstream from Cheney Reservoir site. Newly developed models and 14 years of hourly continuously measured data were used to calculate selected constituent concentrations and loads during January 1999 through December 2012. The water-quality information in this report is important to the city of Wichita because it allows the concentrations of many potential pollutants of interest to Cheney Reservoir, including nutrients and sediment, to be estimated in real time and characterized over conditions and time scales that

  17. Environmental Inventory: Little South Fork Cumberland River.

    Science.gov (United States)

    1981-11-01

    one grave was found the skeleton of a young woman with a round piece of shining mica of the type that comes from North Carolina. Many skeletons were...other necessities were made in the home. The spinning wheel and loon were used to make clothing from wool, cotton, and flax. In the sprinq, maple...sorghum, wheat, oats, millet , buckwheat, soybeans and sunflowers. Grasses and legumes are domestic perennial grasses and herbaceous le- gumes that

  18. North Fork Snoqualmie River Basin Wildlife Study.

    Science.gov (United States)

    1981-03-01

    oil 64 C 7E c .5 t "dI 0 C...115 66 >CL 00 C7 .I 0 pie f- he IV " I_ _ _ oJ m 00 02 OIL 67 -4 -4 C: f0 0 a;a Ř 68 i2 :3 0 $ 1 I , 4 o I.. - we 0 gig 0 I 0 I; 69 -I0 0j C/) 0 0 0...Ruby-crowned Kinglet Regulus calendula Cedar Waxwing Bombycilla cedrorum Starling Sturnus vulgaris Hutton’s Vireo Vireo huttoni Warbling Vireo

  19. Did mosasaurs have forked tongues?

    NARCIS (Netherlands)

    Schulp, Anne S.; Mulder, E. W. A.; Schwenk, K.

    Ever since the first mosasaur restorations were published, these extinct marine reptiles have been pictured with either notched, forked or undivided tongues. Here, we present an overview of existing iconography, a review of the previous literature, and we discuss how best to reconstruct tongue form

  20. Salmon River Habitat Enhancement, 1989 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rowe, Mike

    1989-04-01

    This project was funded by the Bonneville Power Administration (BPA). The annual report contains three individual subproject papers detailing tribal fisheries work completed during the summer and fall of 1989. Subproject 1 contains summaries of evaluation/monitoring efforts associated with the Bear Valley Creek, Idaho enhancement project. Subproject 2 contains an evaluation of the Yankee Fork of the Salmon River habitat enhancement project. This report has been sub-divided into two parts: Part 1; stream evaluation and Part 2; pond series evaluation. Subproject 3 concerns the East Fork of the Salmon River, Idaho. This report summarizes the evaluation of the project to date including the 1989 pre-construction evaluation conducted within the East Fork drainage. Dredge mining has degraded spawning and rearing habitat for chinook salmon and steelhead trout in the Yankee Fork drainage of the Salmon River and in Bear Valley Creek. Mining, agricultural, and grazing practices degraded habitat in the East Fork of the Salmon River. Biological monitoring of the success of habitat enhancement for Bear Valley Creek and Yankee Fork are presented in this report. Physical and biological inventories prior to habitat enhancement in East Fork were also conducted. Four series of off-channel ponds of the Yankee Fork are shown to provide effective rearing habitat for chinook salmon. 45 refs., 49 figs., 24 tabs.

  1. Burnup verification using the FORK measurement system

    International Nuclear Information System (INIS)

    Ewing, R.I.

    1994-01-01

    Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. The FORK measurement system, designed at Los Alamos National Laboratory for the International Atomic Energy Agency safeguards program, has been used to verify reactor site records for burnup and cooling time for many years. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. This report deals with the application of the FORK system to burnup credit operations based on measurements performed on spent fuel assemblies at the Oconee Nuclear Station of Duke Power Company

  2. Code Forking, Governance, and Sustainability in Open Source Software

    OpenAIRE

    Juho Lindman; Linus Nyman

    2013-01-01

    The right to fork open source code is at the core of open source licensing. All open source licenses grant the right to fork their code, that is to start a new development effort using an existing code as its base. Thus, code forking represents the single greatest tool available for guaranteeing sustainability in open source software. In addition to bolstering program sustainability, code forking directly affects the governance of open source initiatives. Forking, and even the mere possibilit...

  3. Evaluate Status of Pacific Lamprey in the Clearwater River Drainage, Idaho, Annual Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Cochnauer, Tim; Claire, Christopher

    2003-10-01

    In 2002 Idaho Department of Fish and Game continued investigation into the status of Pacific lamprey populations in Idaho's Clearwater River drainage. Trapping, electrofishing, and spawning ground redd surveys were used to determine Pacific lamprey distribution, life history strategies, and habitat requirements in the South Fork Clearwater River, Lochsa River, Selway River, and Middle Fork Clearwater River subbasins. Five-hundred forty-one ammocoetes were captured electroshocking 70 sites in the South Fork Clearwater River, Lochsa River, Selway River, Middle Fork Clearwater River, Clearwater River, and their tributaries in 2002. Habitat utilization surveys in Red River support previous work indicating Pacific lamprey ammocoete densities are greater in lateral scour pool habitats compared to riffles and rapids. Presence-absence survey findings in 2002 augmented 2000 and 2001 indicating Pacific lamprey macrothalmia and ammocoetes are not numerous or widely distributed. Pacific lamprey distribution was confined to the lower reaches of Red River below rkm 8.0, the South Fork Clearwater River, Lochsa River (Ginger Creek to mouth), Selway River (Race Creek to mouth), Middle Fork Clearwater River, and the Clearwater River (downstream to Potlatch River).

  4. Henrys Fork near Ashton, ID (YHEN)

    Data.gov (United States)

    Department of the Interior — Henrys Fork near Ashton, Idaho (YHEN) Sample Collection: Samples were collected near the USGS stream gage 13046000 (Latitude 44°04'11", Longitude 111°30'38" NAD83)....

  5. Salmon River Habitat Enhancement. 1990 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Rowe, Mike

    1991-12-01

    The annual report contains three individual subproject sections detailing tribal fisheries work completed during the summer and fall of 1990. Subproject I contains summaries of evaluation/monitoring efforts associated with the Bear Valley Creek, Idaho enhancement project. Subproject II contains an evaluation of the Yankee Fork of the Salmon River habitat enhancement project. Subproject III concerns the East Fork of the Salmon River, Idaho.

  6. The Relationship Between Land Management, Fecal Indicator Bacteria, and the Occurrence of Campylobacter and Listeria Spp. in Water and Sediments During Synoptic Sampling In The South Fork Broad River Watershed, Northeast Georgia, U.S.A.

    Science.gov (United States)

    Fecal indicator bacteria (FIB) and pathogens stored in the bed sediments of streams and rivers may be mobilized into the water column affecting overall water quality. Furthermore, land management may play an important role in the concentrations of FIB and the occurrence of pathog...

  7. FBH1 Catalyzes Regression of Stalled Replication Forks

    Directory of Open Access Journals (Sweden)

    Kasper Fugger

    2015-03-01

    Full Text Available DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal. Here, we show that FBH1 catalyzes regression of a model replication fork in vitro and promotes fork regression in vivo in response to replication perturbation. Cells respond to fork stalling by activating checkpoint responses requiring signaling through stress-activated protein kinases. Importantly, we show that FBH1, through its helicase activity, is required for early phosphorylation of ATM substrates such as CHK2 and CtIP as well as hyperphosphorylation of RPA. These phosphorylations occur prior to apparent DNA double-strand break formation. Furthermore, FBH1-dependent signaling promotes checkpoint control and preserves genome integrity. We propose a model whereby FBH1 promotes early checkpoint signaling by remodeling of stalled DNA replication forks.

  8. Pyrimidine dimers block simian virus 40 replication forks

    International Nuclear Information System (INIS)

    Berger, C.A.; Edenberg, H.J.

    1986-01-01

    UV light produces lesions, predominantly pyrimidine dimers, which inhibit DNA replication in mammalian cells. The mechanism of inhibition is controversial: is synthesis of a daughter strand halted at a lesion while the replication fork moves on and reinitiates downstream, or is fork progression itself blocked for some time at the site of a lesion? We directly addressed this question by using electron microscopy to examine the distances of replication forks from the origin in unirradiated and UV-irradiated simian virus 40 chromosomes. If UV lesions block replication fork progression, the forks should be asymmetrically located in a large fraction of the irradiated molecules; if replication forks move rapidly past lesions, the forks should be symmetrically located. A large fraction of the simian virus 40 replication forks in irradiated molecules were asymmetrically located, demonstrating that UV lesions present at the frequency of pyrimidine dimers block replication forks. As a mechanism for this fork blockage, we propose that polymerization of the leading strand makes a significant contribution to the energetics of fork movement, so any lesion in the template for the leading strand which blocks polymerization should also block fork movement

  9. DNA fork displacement rates in human cells

    International Nuclear Information System (INIS)

    Kapp, L.N.; Painter, R.B.

    1981-01-01

    DNA fork displacement rates were measured in 20 human cell lines by a bromodeoxyuridine-313 nm photolysis technique. Cell lines included representatives of normal diploid, Fanconi's anemia, ataxia telangiectasia, xeroderma pigmentosum, trisomy-21 and several transformed lines. The average value for all the cell lines was 0.53 +- 0.08 μm/min. The average value for individual cell lines, however, displayed a 30% variation. Less than 10% of variation in the fork displacement rate appears to be due to the experimental technique; the remainder is probably due to true variation among the cell types and to culture conditions. (Auth.)

  10. DNA fork displacement rates in human cells

    Energy Technology Data Exchange (ETDEWEB)

    Kapp, L.N.; Painter, R.B. (California Univ., San Francisco (USA). Lab. of Radiobiology)

    1981-11-27

    DNA fork displacement rates were measured in 20 human cell lines by a bromodeoxyuridine-313 nm photolysis technique. Cell lines included representatives of normal diploid, Fanconi's anemia, ataxia telangiectasia, xeroderma pigmentosum, trisomy-21 and several transformed lines. The average value for all the cell lines was 0.53 +- 0.08 ..mu..m/min. The average value for individual cell lines, however, displayed a 30% variation. Less than 10% of variation in the fork displacement rate appears to be due to the experimental technique; the remainder is probably due to true variation among the cell types and to culture conditions.

  11. Development of the Pintle Release Fork Mechanism

    International Nuclear Information System (INIS)

    BOGER, R.M.; DALE, R.

    1999-01-01

    An improved method of attachment of the pintle to the piston in the universal sampler is being developed. The mechanism utilizes a forked release disk which captures two balls in a cavity formed by a hole in the piston and a groove in the pintle rod

  12. Malheur River Basin cooperative bull trout/redband trout research project, annual report FY 1999; ANNUAL

    International Nuclear Information System (INIS)

    Schwabe, Lawrence; Tiley, Mark

    2000-01-01

    The purpose of this study is to document the seasonal distribution of adult/sub-adult bull trout (Salvelinus confluentus) in the Malheur River basin. Due to the decline of bull trout in the Columbia Basin, the United States Fish and Wildlife Service listed bull trout as a threatened species in June 1998. Past land management activities; construction of dams; and fish eradication projects in the North Fork and Middle Fork Malheur River by poisoning have worked in concert to cumulatively impact native species in the Malheur Basin (Bowers et. al. 1993). Survival of the remaining bull trout populations is severely threatened (Buchanan 1997). 1999 Research Objects are: (1) Document the migratory patterns of adult/sub-adult bull trout in the North Fork Malheur River; (2) Determine the seasonal bull trout use of Beulah Reservoir and bull trout entrainment; and (3) Timing and location of bull trout spawning in the North Fork Malheur River basin. The study area includes the Malheur basin from the mouth of the Malheur River located near Ontario, Oregon to the headwaters of the North Fork Malheur River (Map 1). All fish collected and most of the telemetry effort was done on the North Fork Malheur River subbasin (Map 2). Fish collection was conducted on the North Fork Malheur River at the tailwaters of Beulah Reservoir (RK 29), Beulah Reservoir (RK 29-RK 33), and in the North Fork Malheur River at Crane Crossing (RK 69) to the headwaters of the North Fork Malheur. Radio telemetry was done from the mouth of the Malheur River in Ontario, Oregon to the headwaters of the North Fork Malheur. This report will reflect all migration data collected from 3/1/99 to 12/31/99

  13. Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery.

    Science.gov (United States)

    Murphy, Anar K; Fitzgerald, Michael; Ro, Teresa; Kim, Jee Hyun; Rabinowitsch, Ariana I; Chowdhury, Dipanjan; Schildkraut, Carl L; Borowiec, James A

    2014-08-18

    Phosphorylation of replication protein A (RPA) by Cdk2 and the checkpoint kinase ATR (ATM and Rad3 related) during replication fork stalling stabilizes the replisome, but how these modifications safeguard the fork is not understood. To address this question, we used single-molecule fiber analysis in cells expressing a phosphorylation-defective RPA2 subunit or lacking phosphatase activity toward RPA2. Deregulation of RPA phosphorylation reduced synthesis at forks both during replication stress and recovery from stress. The ability of phosphorylated RPA to stimulate fork recovery is mediated through the PALB2 tumor suppressor protein. RPA phosphorylation increased localization of PALB2 and BRCA2 to RPA-bound nuclear foci in cells experiencing replication stress. Phosphorylated RPA also stimulated recruitment of PALB2 to single-strand deoxyribonucleic acid (DNA) in a cell-free system. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. These data demonstrate that phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress. © 2014 Murphy et al.

  14. Homologous Recombination as a Replication Fork Escort: Fork-Protection and Recovery

    Directory of Open Access Journals (Sweden)

    Audrey Costes

    2012-12-01

    Full Text Available Homologous recombination is a universal mechanism that allows DNA repair and ensures the efficiency of DNA replication. The substrate initiating the process of homologous recombination is a single-stranded DNA that promotes a strand exchange reaction resulting in a genetic exchange that promotes genetic diversity and DNA repair. The molecular mechanisms by which homologous recombination repairs a double-strand break have been extensively studied and are now well characterized. However, the mechanisms by which homologous recombination contribute to DNA replication in eukaryotes remains poorly understood. Studies in bacteria have identified multiple roles for the machinery of homologous recombination at replication forks. Here, we review our understanding of the molecular pathways involving the homologous recombination machinery to support the robustness of DNA replication. In addition to its role in fork-recovery and in rebuilding a functional replication fork apparatus, homologous recombination may also act as a fork-protection mechanism. We discuss that some of the fork-escort functions of homologous recombination might be achieved by loading of the recombination machinery at inactivated forks without a need for a strand exchange step; as well as the consequence of such a model for the stability of eukaryotic genomes.

  15. Grand Forks - East Grand Forks Urban Water Resources Study. Grand Forks Flood Fight Manual

    Science.gov (United States)

    1981-07-01

    insurance is available for residential and commercial structures and contents at actuarial rates. As of 30 April 1980, 1258 policies were in effect...STATION #3 (WW) CLOSE SHEAR OATE ON LEW« M.VD. ON RIVER SOE OF IMKE. (S) PUCE PUMP M CATCH ■ ASM ON OTHER «DC OF MKE CLOSE SHEAR OATE ON OKE AT ISIS mVBMM...DRIVE. (S>* PUCE PUMP M CATCH « ASM ON OTHER SBC OF (NKE CLOU SHEAR OATE AT UFT STATION «S M MANHOU «WW> SWITCH OVER UFT STATION «I - CLOSE OATE

  16. Captive Rearing Initiative for Salmon River Chinook Salmon, 1998-1999 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Hassemer, Peter F.

    2001-04-01

    During 1999, the Idaho Department of Fish and Game (IDFG) continued developing techniques for the captive rearing of chinook salmon Oncorhynchus tshawytscha. Techniques under development included protocols for rearing juveniles in freshwater and saltwater hatchery environments, and fieldwork to collect brood year 1998 and 1999 juveniles and eggs and to investigate the ability of these fish to spawn naturally. Fish collected as juveniles were held for a short time at the Sawtooth Fish Hatchery and later transferred to the Eagle Fish Hatchery for rearing. Eyed-eggs were transferred immediately to the Eagle Fish Hatchery where they were disinfected and reared by family groups. When fish from either collection method reached approximately 60 mm, they were PIT tagged and reared separately by brood year and source stream. Sixteen different groups were in culture at IDFG facilities in 1999. Hatchery spawning activities of captive-reared chinook salmon produced eyed-eggs for outplanting in streamside incubation chambers in the West Fork Yankee Fork Salmon River (N=2,297) and the East Fork Salmon River (N=1,038). Additionally, a number of these eggs were maintained at the Eagle Fish Hatchery to ensure adequate brood year 1999 representation from these systems, and produced 279 and 87 juveniles from the West Fork Yankee Fork and East Fork Salmon River, respectively. Eyed-eggs were not collected from the West Fork Yankee Fork due to low adult escapement. Brood year 1998 juveniles were collected from the Lemhi River (N=191), West Fork Yankee Fork Salmon River (N=229), and East Fork Salmon River (N=185). Additionally, brood year 1999 eyed-eggs were collected from the Lemhi River (N=264) and East Fork Salmon River (N=143). Sixty-two and seven maturing adults were released into Bear Valley Creek (Lemhi River system) and the East Fork Salmon River, respectively, for spawning evaluation in 1999. Nine female carcasses from Bear Valley Creek were examined for egg retention, and of

  17. Captive Rearing Initiative for Salmon River Chinook Salmon, 1999 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Hassemer, Peter F.

    2001-04-01

    During 1999, the Idaho Department of Fish and Game (IDFG) continued developing techniques for the captive rearing of chinook salmon Oncorhynchus tshawytscha. Techniques under development included protocols for rearing juveniles in freshwater and saltwater hatchery environments, and fieldwork to collect brood year 1998 and 1999 juveniles and eggs and to investigate the ability of these fish to spawn naturally. Fish collected as juveniles were held for a short time at the Sawtooth Fish Hatchery and later transferred to the Eagle Fish Hatchery for rearing. Eyed-eggs were transferred immediately to the Eagle Fish Hatchery where they were disinfected and reared by family groups. When fish from either collection method reached approximately 60 mm, they were PIT tagged and reared separately by brood year and source stream. Sixteen different groups were in culture at IDFG facilities in 1999. Hatchery spawning activities of captive-reared chinook salmon produced eyed-eggs for outplanting in streamside incubation chambers in the West Fork Yankee Fork Salmon River (N=2,297) and the East Fork Salmon River (N=1,038). Additionally, a number of these eggs were maintained at the Eagle Fish Hatchery to ensure adequate brood year 1999 representation from these systems, and produced 279 and 87 juveniles from the West Fork Yankee Fork and East Fork Salmon River, respectively. Eyed-eggs were not collected from the West Fork Yankee Fork due to low adult escapement. Brood year 1998 juveniles were collected from the Lemhi River (N=191), West Fork Yankee Fork Salmon River (N=229), and East Fork Salmon River (N=185). Additionally, brood year 1999 eyed-eggs were collected from the Lemhi River (N=264) and East Fork Salmon River (N=143). Sixty-two and seven maturing adults were released into Bear Valley Creek (Lemhi River system) and the East Fork Salmon River, respectively, for spawning evaluation in 1999. Nine female carcasses from Bear Valley Creek were examined for egg retention, and of

  18. Code Forking, Governance, and Sustainability in Open Source Software

    Directory of Open Access Journals (Sweden)

    Juho Lindman

    2013-01-01

    Full Text Available The right to fork open source code is at the core of open source licensing. All open source licenses grant the right to fork their code, that is to start a new development effort using an existing code as its base. Thus, code forking represents the single greatest tool available for guaranteeing sustainability in open source software. In addition to bolstering program sustainability, code forking directly affects the governance of open source initiatives. Forking, and even the mere possibility of forking code, affects the governance and sustainability of open source initiatives on three distinct levels: software, community, and ecosystem. On the software level, the right to fork makes planned obsolescence, versioning, vendor lock-in, end-of-support issues, and similar initiatives all but impossible to implement. On the community level, forking impacts both sustainability and governance through the power it grants the community to safeguard against unfavourable actions by corporations or project leaders. On the business-ecosystem level forking can serve as a catalyst for innovation while simultaneously promoting better quality software through natural selection. Thus, forking helps keep open source initiatives relevant and presents opportunities for the development and commercialization of current and abandoned programs.

  19. The forked flap repair for hypospadias

    Directory of Open Access Journals (Sweden)

    Anil Chadha

    2012-01-01

    Full Text Available Context: Despite the abundance of techniques for the repair of Hypospadias, its problems still persist and a satisfactory design to correct the penile curvature with the formation of neourethra from the native urethral tissue or genital or extragenital tissues, with minimal postoperative complications has yet to evolve. Aim: Persisting with such an endeavor, a new technique for the repair of distal and midpenile hypospadias is described. Materials and Methods: The study has been done in 70 cases over the past 11 years. The "Forked-Flap" repair is a single stage method for the repair of such Hypospadias with chordee. It takes advantage of the rich vascular communication at the corona and capitalizes on the established reliability of the meatal based flip-flap. The repair achieves straightening of the curvature of the penis by complete excision of chordee tissue from the ventral surface of the penis beneath the urethral plate. The urethra is reconstructed using the native plate with forked flap extensions and genital tissue relying on the concept of meatal based flaps. Water proofing by dartos tissue and reinforcement by Nesbit′s prepucial tissue transfer completes the one stage procedure. Statistical Analysis: An analysis of 70 cases of this single stage technique of repair of penile hypospadias with chordee, operated at 3 to 5 years of age over the past 11 years is presented. Results and Conclusion: The Forked Flap gives comparable and replicable results; except for a urethrocutaneous fistula rate of 4% no other complications were observed.

  20. Structural design of a composite bicycle fork

    International Nuclear Information System (INIS)

    Baldissera, Paolo; Delprete, Cristiana

    2014-01-01

    Highlights: • Case study about composite bicycle fork design. • Special requirements for a Student Team project. • FE model to evaluate stiffness, strength and potential failure modes. • Comparison of two manufacturing approaches. • FE model stiffness validation on the manufactured fork. - Abstract: Despite the wide literature on the mechanical behaviour of carbon/epoxy composites, it is rare to find practical methodological approaches in finite element design of structural components made by laminate layup. Through the case study of a special bicycle fork needed in a Student Team prototype, this paper proposes a simplified methodology as starting point for educational and manufacturing purposes. In order to compare two manufacturing solutions in terms of stiffness, strength and failure mode, a numerical model was implemented. Since the project requirements imposed to avoid standard destructive testing, the model validation was based on a posteriori linear stiffness comparison with the manufactured component. The slight discrepancies between experimental and numerical results were discussed in order to check their origin and to assess the reliability of the model. The overall methodology, even if complain with only a part of the safety standard requirements, shows to be reliable enough and can be the basis for further extension and refinement

  1. Regulation of replication fork progression through histone supply and demand

    DEFF Research Database (Denmark)

    Groth, Anja; Corpet, Armelle; Cook, Adam J L

    2007-01-01

    DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone c...... progression and histone supply and demand.......1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork...

  2. Economic impacts of guided whitewater rafting: a study of five rivers

    Science.gov (United States)

    Donald B.K. English; J. Michael Bowker

    1996-01-01

    This paper presents estimates of the statewide economic impacts of guided whitewater rafting on five rivers in six states: the Nantahala (North Carolina), Gauley (West Virginia), Kennebec (Maine), Middle Fork of the Salmon (Idaho), and Chattooga (Georgia-South Carolina). Except for the Chattooga and Middle Fork, rafting is dependent on upstream dam releases. Guide fees...

  3. Hance_WestForkSmithRiver_flasher_location_data

    Data.gov (United States)

    U.S. Environmental Protection Agency — This entry contains two files. The first file, "Hance_WFSR Flasher locations.xlxs", contains information describing the location of installed landmark 'flashers'...

  4. Evaluation of the Life History of Native Salmonids in the Malheur River Basin; Cooperative Bull Trout/Redband Trout Research Project, 1999-2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Schwabe, Lawrence; Tiley, Mark (Burns Paiute Tribe, Department of Fish and Wildlife, Burns, OR); Perkins, Raymond R. (Oregon Department of Fish and Wildlife, Ontario, OR)

    2000-11-01

    The purpose of this study is to document the seasonal distribution of adult/sub-adult bull trout (Salvelinus confluentus) in the Malheur River basin. Due to the decline of bull trout in the Columbia Basin, the United States Fish and Wildlife Service listed bull trout as a threatened species in June 1998. Past land management activities; construction of dams; and fish eradication projects in the North Fork and Middle Fork Malheur River by poisoning have worked in concert to cumulatively impact native species in the Malheur Basin (Bowers et. al. 1993). Survival of the remaining bull trout populations is severely threatened (Buchanan 1997). 1999 Research Objects are: (1) Document the migratory patterns of adult/sub-adult bull trout in the North Fork Malheur River; (2) Determine the seasonal bull trout use of Beulah Reservoir and bull trout entrainment; and (3) Timing and location of bull trout spawning in the North Fork Malheur River basin. The study area includes the Malheur basin from the mouth of the Malheur River located near Ontario, Oregon to the headwaters of the North Fork Malheur River (Map 1). All fish collected and most of the telemetry effort was done on the North Fork Malheur River subbasin (Map 2). Fish collection was conducted on the North Fork Malheur River at the tailwaters of Beulah Reservoir (RK 29), Beulah Reservoir (RK 29-RK 33), and in the North Fork Malheur River at Crane Crossing (RK 69) to the headwaters of the North Fork Malheur. Radio telemetry was done from the mouth of the Malheur River in Ontario, Oregon to the headwaters of the North Fork Malheur. This report will reflect all migration data collected from 3/1/99 to 12/31/99.

  5. Mass loading of selected major and trace elements in Lake Fork Creek near Leadville, Colorado, September-October 2001

    Science.gov (United States)

    Walton-Day, Katherine; Flynn, Jennifer L.; Kimball, Briant A.; Runkel, Robert L.

    2005-01-01

    A mass-loading study of Lake Fork Creek of the Arkansas River between Sugarloaf Dam and the mouth was completed in September-October 2001 to help ascertain the following: (1) variation of pH and aqueous constituent concentrations (calcium, sulfate, alkalinity, aluminum, cadmium, copper, iron, manganese, lead, and zinc) and their relation to toxicity standards along the study reach; (2) location and magnitude of sources of metal loading to Lake Fork Creek; (3) amount and locations of metal attenuation; (4) the effect of streamside wetlands on metal transport from contributing mine tunnels; and (5) the effect of organic-rich inflow from the Leadville National Fish Hatchery on water quality in Lake Fork Creek. The study was done in cooperation with the Bureau of Land Management, U.S. Department of Agriculture Forest Service, and U.S. Fish and Wildlife Service. Constituent concentrations and pH showed variable patterns over the study reach. Hardness-based acute and chronic toxicity standards were exceeded for some inflows and some constituents. However, stream concentrations did not exceed standards except for zinc starting in the upper parts of the study reach and extending to just downstream from the inflow from the Leadville National Fish Hatchery. Dilution from that inflow lowered stream zinc concentrations to less than acute and chronic toxicity standards. The uppermost 800 meters of the study reach that contained inflow from the Bartlett, Dinero, and Nelson mine tunnels and the Dinero wetland was the greatest source of loading for manganese and zinc. A middle section of the study reach that extended approximately 2 kilometers upstream from the National Fish Hatchery inflow to just downstream from that inflow was the largest source of aluminum, copper, iron, and lead loading. The loading was partially from the National Fish Hatchery inflow but also from unknown sources upstream from that inflow, possibly ground water. The largest sources for calcium and sulfate

  6. Movement of bull trout in the upper Jarbidge River watershed, Idaho and Nevada, 2008-09--A supplement to Open-File Report 2010-1033

    Science.gov (United States)

    Munz, Carrie S.; Allen, M. Brady; Connolly, Patrick J.

    2011-01-01

    We monitored bull trout (Salvelinus confluentus) in 2008 and 2009 as a continuation of our work in 2006 and 2007, which involved the tagging of 1,536 bull trout with passive integrated transponder (PIT) tags in the East Fork Jarbidge River and West Fork Jarbidge River and their tributaries in northeastern Nevada and southern Idaho. We installed PIT tag interrogation systems (PTISs) at established locations soon after ice-out, and maintained the PTISs in order to collect information on bull trout movements through December of each year. We observed a marked increase of movement in 2008 and 2009. Bull trout tagged in the uppermost portions of the East Fork Jarbidge River at altitudes greater than 2,100 meters moved to the confluence of the East Fork Jarbidge River and West Fork Jarbidge River in summer and autumn. Ten bull trout tagged upstream of the confluence of Pine Creek and the West Fork Jarbidge River moved downstream and then upstream in the East Fork Jarbidge River, and then past the PTIS at Murphy Hot Springs (river kilometer [rkm] 4.1). Two of these fish ascended Dave Creek, a tributary of the East Fork Jarbidge River, past the PTIS at rkm 0.4. One bull trout that was tagged at rkm 11 in Dave Creek on June 28, 2007 moved downstream to the confluence of the East Fork Jarbidge River and West Fork Jarbidge River (rkm 0) on July 28, 2007, and it was then detected in the West Fork Jarbidge River moving past our PTIS at rkm 15 on May 4, 2008. Combined, the extent and types of bull trout movements observed indicated that the primarily age-1 and age-2 bull trout that we tagged in 2006 and 2007 showed increased movement with age and evidence of a substantial amount of fluvial life history. The movements suggest strong connectivity between spawning areas and downstream mainstem areas, as well as between the East Fork Jarbidge River and West Fork Jarbidge River.

  7. Development of Tuning Fork Based Probes for Atomic Force Microscopy

    Science.gov (United States)

    Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

    2014-03-01

    This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

  8. Chemical and biological sensing using tuning forks

    Science.gov (United States)

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  9. Growth Data - Captive Broodstock Gene Rescue Program for Odd Year Class Elwha River Pink Salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conduct captive brood stock gene rescue program for Elwha River odd-year class pink salmon. The fork length to the nearest mm and weight to the nearest gram of a...

  10. Spent-fuel verification with the Los Alamos fork detector

    International Nuclear Information System (INIS)

    Rinard, P.M.; Bosler, G.E.

    1987-01-01

    The Los Alamos fork detector for the verification of spent-fuel assemblies has generated precise, reproducible data. The data analyses have now evolved to the point of placing tight restrictions on a diverter's actions

  11. Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork

    OpenAIRE

    Rapp, Jordan B.; Ansbach, Alison B.; Noguchi, Chiaki; Noguchi, Eishi

    2009-01-01

    Replication of chromosomes involves a variety of replication proteins including DNA polymerases, DNA helicases, and other accessory factors. Many of these proteins are known to localize at replication forks and travel with them as components of the replisome complex. Other proteins do not move with replication forks but still play an essential role in DNA replication. Therefore, in order to understand the mechanisms of DNA replication and its controls, it is important to examine localization ...

  12. Force regulated dynamics of RPA on a DNA fork.

    Science.gov (United States)

    Kemmerich, Felix E; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

    2016-07-08

    Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg(2+) concentrations, such that human RPA can melt DNA in absence of force. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  13. Bull Trout Population Assessment in the White Salmon and Klickitat Rivers, Columbia River Gorge, Washington, 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Thiesfeld, Steven L.; McPeak, Ronald H.; McNamara, Brian S. (Washington Department of Fish and Wildlife); Honanie, Isadore (Confederated Tribes and Bands, Yakama Nation)

    2002-01-01

    We utilized night snorkeling and single pass electroshocking to determine the presence or absence of bull trout Salvelinus confluentus in 26 stream reaches (3,415 m) in the White Salmon basin and in 71 stream reaches (9,005 m) in the Klickitat River basin during summer and fall 2001. We did not find any bull trout in the White Salmon River basin. In the Klickitat River basin, bull trout were found only in the West Fork Klickitat River drainage. We found bull trout in two streams not previously reported: Two Lakes Stream and an unnamed tributary to Fish Lake Stream (WRIA code number 30-0550). We attempted to capture downstream migrant bull trout in the West Fork Klickitat River by fishing a 1.5-m rotary screw trap at RM 4.3 from July 23 through October 17. Although we caught other salmonids, no bull trout were captured. The greatest limiting factor for bull trout in the West Fork Klickitat River is likely the small amount of available habitat resulting in a low total abundance, and the isolation of the population. Many of the streams are fragmented by natural falls, which are partial or complete barriers to upstream fish movement. To date, we have not been able to confirm that the occasional bull trout observed in the mainstem Klickitat River are migrating upstream into the West Fork Klickitat River.

  14. Advancing the Fork detector for quantitative spent nuclear fuel verification

    Science.gov (United States)

    Vaccaro, S.; Gauld, I. C.; Hu, J.; De Baere, P.; Peterson, J.; Schwalbach, P.; Smejkal, A.; Tomanin, A.; Sjöland, A.; Tobin, S.; Wiarda, D.

    2018-04-01

    The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations. A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This paper describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms

  15. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe.

    Science.gov (United States)

    Margalef, Pol; Kotsantis, Panagiotis; Borel, Valerie; Bellelli, Roberto; Panier, Stephanie; Boulton, Simon J

    2018-01-25

    Telomere maintenance critically depends on the distinct activities of telomerase, which adds telomeric repeats to solve the end replication problem, and RTEL1, which dismantles DNA secondary structures at telomeres to facilitate replisome progression. Here, we establish that reversed replication forks are a pathological substrate for telomerase and the source of telomere catastrophe in Rtel1 -/- cells. Inhibiting telomerase recruitment to telomeres, but not its activity, or blocking replication fork reversal through PARP1 inhibition or depleting UBC13 or ZRANB3 prevents the rapid accumulation of dysfunctional telomeres in RTEL1-deficient cells. In this context, we establish that telomerase binding to reversed replication forks inhibits telomere replication, which can be mimicked by preventing replication fork restart through depletion of RECQ1 or PARG. Our results lead us to propose that telomerase inappropriately binds to and inhibits restart of reversed replication forks within telomeres, which compromises replication and leads to critically short telomeres. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  16. Forked and Integrated Variants In An Open-Source Firmware Project

    DEFF Research Database (Denmark)

    Stanciulescu, Stefan; Schulze, Sandro; Wasowski, Andrzej

    2015-01-01

    and interactive source management platforms such as Github. We study advantages and disadvantages of forking using the case of Marlin, an open source firmware for 3D printers. We find that many problems and advantages of cloning do translate to forking. Interestingly, the Marlin community uses both forking......Code cloning has been reported both on small (code fragments) and large (entire projects) scale. Cloning-in-the-large, or forking, is gaining ground as a reuse mechanism thanks to availability of better tools for maintaining forked project variants, hereunder distributed version control systems...

  17. A Transimpedance Amplifier for Remotely Located Quartz Tuning Forks

    OpenAIRE

    Kleinbaum, Ethan; Csathy, Gabor

    2012-01-01

    The cable capacitance in cryogenic and high vacuum applications of quartz tuning forks imposes severe constraints on the bandwidth and noise performance of the measurement. We present a single stage low noise transimpedance amplifier with a bandwidth exceeding 1 MHz and provide an in-depth analysis of the dependence of the amplifier parameters on the cable capacitance.

  18. Note: a transimpedance amplifier for remotely located quartz tuning forks.

    Science.gov (United States)

    Kleinbaum, Ethan; Csáthy, Gábor A

    2012-12-01

    The cable capacitance in cryogenic and high vacuum applications of quartz tuning forks imposes severe constraints on the bandwidth and noise performance of the measurement. We present a single stage low noise transimpedance amplifier with a bandwidth exceeding 1 MHz and provide an in-depth analysis of the dependence of the amplifier parameters on the cable capacitance.

  19. Flood disaster preparedness: a retrospect from Grand Forks, North Dakota.

    Science.gov (United States)

    Siders, C; Jacobson, R

    1998-01-01

    Natural disasters often come without warning. The clinical, financial, and business risks can be enormous. Grand Forks' (ND) healthcare systems experienced a flooding disaster of unprecedented proportions in April of 1997. Planned and practiced disaster and evacuation procedures can significantly reduce a healthcare facilities' risk to life, health, and safety. This article retrospectively analyzes disaster preparation and the complete evacuation of the facilities' patients.

  20. Assembly of Slx4 signaling complexes behind DNA replication forks.

    Science.gov (United States)

    Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

    2015-08-13

    Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress. © 2015 The Authors.

  1. Bull trout population assessment in the White Salmon and Klickitat Rivers, Columbia River Gorge, Washington; ANNUAL fiscal year 2001 annual report

    International Nuclear Information System (INIS)

    Thiesfield, Steven L.

    2002-01-01

    We utilized night snorkeling and single pass electroshocking to determine the presence or absence of bull trout Salvelinus confluentus in 26 stream reaches (3,415 m) in the White Salmon basin and in 71 stream reaches (9,005 m) in the Klickitat River basin during summer and fall 2001. We did not find any bull trout in the White Salmon River basin. In the Klickitat River basin, bull trout were found only in the West Fork Klickitat River drainage. We found bull trout in two streams not previously reported: Two Lakes Stream and an unnamed tributary to Fish Lake Stream (WRIA code number 30-0550). We attempted to capture downstream migrant bull trout in the West Fork Klickitat River by fishing a 1.5-m rotary screw trap at RM 4.3 from July 23 through October 17. Although we caught other salmonids, no bull trout were captured. The greatest limiting factor for bull trout in the West Fork Klickitat River is likely the small amount of available habitat resulting in a low total abundance, and the isolation of the population. Many of the streams are fragmented by natural falls, which are partial or complete barriers to upstream fish movement. To date, we have not been able to confirm that the occasional bull trout observed in the mainstem Klickitat River are migrating upstream into the West Fork Klickitat River

  2. Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

    Science.gov (United States)

    Schalbetter, Stephanie A; Mansoubi, Sahar; Chambers, Anna L; Downs, Jessica A; Baxter, Jonathan

    2015-08-18

    Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs frequently during elongation. However, the factors that influence fork rotation and how rotation and precatenation may influence other replication-associated processes are unknown. Here we analyze the causes and consequences of fork rotation in budding yeast. We find that fork rotation and precatenation preferentially occur in contexts that inhibit topoisomerase action ahead of the fork, including stable protein-DNA fragile sites and termination. However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3. In the absence of Tof1/Timeless, excessive fork rotation and precatenation cause extensive DNA damage following DNA replication. With Tof1, damage related to precatenation is focused on the fragile protein-DNA sites where fork rotation is induced. We conclude that although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin.

  3. RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks.

    Science.gov (United States)

    Elia, Andrew E H; Wang, David C; Willis, Nicholas A; Boardman, Alexander P; Hajdu, Ildiko; Adeyemi, Richard O; Lowry, Elizabeth; Gygi, Steven P; Scully, Ralph; Elledge, Stephen J

    2015-10-15

    We have used quantitative proteomics to profile ubiquitination in the DNA damage response (DDR). We demonstrate that RPA, which functions as a protein scaffold in the replication stress response, is multiply ubiquitinated upon replication fork stalling. Ubiquitination of RPA occurs on chromatin, involves sites outside its DNA binding channel, does not cause proteasomal degradation, and increases under conditions of fork collapse, suggesting a role in repair at stalled forks. We demonstrate that the E3 ligase RFWD3 mediates RPA ubiquitination. RFWD3 is necessary for replication fork restart, normal repair kinetics during replication stress, and homologous recombination (HR) at stalled replication forks. Mutational analysis suggests that multisite ubiquitination of the entire RPA complex is responsible for repair at stalled forks. Multisite protein group sumoylation is known to promote HR in yeast. Our findings reveal a similar requirement for multisite protein group ubiquitination during HR at stalled forks in mammalian cells. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. The fecundity of fork-tailed threadfin bream (Nemipterus furcosus) in Bangka, Bangka Belitung

    Science.gov (United States)

    Utami, E.; Safitriyani, E.; Gatra Persada, Leo

    2018-04-01

    Fork-tailed threadfin bream (Nemipterus furcosus) is one of important economic fishes in Bangka. The sustainability of fork-tailed threadfin bream is threatened by degradation of natural habitat. Information of reproductive is needed for further management. The objective of this study was to examine fecundity of fork-tailed threadfin bream. The mean values of temperature was 28.83 ± 0,37°C, respectively. Sex ratio during sampling showed that female fork-tailed threadfin bream greater than male population. Berried female fork-tailed threadfin bream found from March until November. The greatest number of berried female fork-tailed threadfin bream showed in July with berried female value of 25. Fork-tailed threadfin bream fecundity was 19951 and 66628, respectively. The fecundity data can be used to access the reproductive potential of fish stock and also as an assessment on stock size of their natural population.

  5. Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.

    Science.gov (United States)

    Morrow, Carl A; Nguyen, Michael O; Fower, Andrew; Wong, Io Nam; Osman, Fekret; Bryer, Claire; Whitby, Matthew C

    2017-06-06

    Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. Collapsed forks can be rescued by homologous recombination, which restarts replication. However, replication restart is relatively slow and, therefore, replication termination may frequently occur by an active fork converging on a collapsed fork. We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. Based on our findings, we propose that IFSA is a potential threat to genomic stability in eukaryotes.

  6. Termination of DNA replication forks: "Breaking up is hard to do".

    Science.gov (United States)

    Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

    2015-01-01

    To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

  7. Floods of July 23-26, 2010, in the Little Maquoketa River and Maquoketa River Basins, Northeast Iowa

    Science.gov (United States)

    Eash, David A.

    2012-01-01

    Minor flooding occurred July 23, 2010, in the Little Maquoketa River Basin and major flooding occurred July 23–26, 2010, in the Maquoketa River Basin in northeast Iowa following severe thunderstorm activity over the region during July 22–24. A breach of the Lake Delhi Dam on July 24 aggravated flooding on the Maquoketa River. Rain gages at Manchester and Strawberry Point, Iowa, recorded 72-hour-rainfall amounts of 7.33 and 12.23 inches, respectively, on July 24. The majority of the rainfall occurred during a 48-hour period. Within the Little Maquoketa River Basin, a peak-discharge estimate of 19,000 cubic feet per second (annual flood-probability estimate of 4 to 10 percent) at the discontinued 05414500 Little Maquoketa River near Durango, Iowa streamgage on July 23 is the sixth largest flood on record. Within the Maquoketa River Basin, peak discharges of 26,600 cubic feet per second (annual flood-probability estimate of 0.2 to 1 percent) at the 05416900 Maquoketa River at Manchester, Iowa streamgage on July 24, and of 25,000 cubic feet per second (annual flood-probability estimate of 1 to 2 percent) at the 05418400 North Fork Maquoketa River near Fulton, Iowa streamgage on July 24 are the largest floods on record for these sites. A peak discharge affected by the Lake Delhi Dam breach on July 24 at the 05418500 Maquoketa River near Maquoketa, Iowa streamgage, located downstream of Lake Delhi, of 46,000 cubic feet per second on July 26 is the third highest on record. High-water marks were measured at five locations along the Little Maquoketa and North Fork Little Maquoketa Rivers between U.S. Highway 52 near Dubuque and County Road Y21 near Rickardsville, a distance of 19 river miles. Highwater marks were measured at 28 locations along the Maquoketa River between U.S. Highway 52 near Green Island and State Highway 187 near Arlington, a distance of 142 river miles. High-water marks were measured at 13 locations along the North Fork Maquoketa River between

  8. Flathead River creel report, 1992--1993. Final report

    International Nuclear Information System (INIS)

    Hanzel, D.

    1995-09-01

    A roving creel survey was conducted on the Flathead River system, May 1992 through May 1993, as part of Hungry Horse Dam Fisheries Mitigation, funded by Bonneville Power Administration. The Flathead River system is a tributary to the Clarks Fork of the Columbia River originating in northwest Montana and southern British Columbia. The river creel survey was conducted in conjunction with a Flathead Lake creel survey. This document summarizes the creel survey on the river system. The purpose of these creel surveys was to quantify fishery status prior to mitigation efforts and provide replicative survey methodology to measure success of future mitigation activities. 4 figs., 21 tabs

  9. Evaluate Status of Pacific Lamprey in the Clearwater River Drainage, Idaho: Annual Report 2001.

    Energy Technology Data Exchange (ETDEWEB)

    Cochnauer, Tim; Claire, Christopher

    2002-12-01

    Recent decline of Pacific lamprey Lampetra tridentata adult migrants to the Snake River drainage has focused attention on the species. Adult Pacific lamprey counted passing Ice Harbor Dam fishway averaged 18,158 during 1962-69 and 361 during 1993-2000. Human resource manipulations in the Snake River and Clearwater River drainages have altered ecosystem habitat in the last 120 years, likely impacting the productive potential of Pacific lamprey habitat. Timber harvest, stream impoundment, road construction, grazing, mining, and community development have dominated habitat alteration in the Clearwater River system and Snake River corridor. Hydroelectric projects in the Snake River corridor impact juvenile/larval Pacific lamprey outmigrants and returning adults. Juvenile and larval lamprey outmigrants potentially pass through turbines, turbine bypass/collection systems, and over spillway structures at the four lower Snake River hydroelectric dams. Clearwater River drainage hydroelectric facilities have impacted Pacific lamprey populations to an unknown degree. The Pacific Power and Light Dam on the Clearwater River in Lewiston, Idaho, restricted chinook salmon Oncorhynchus tshawytscha passage in the 1927-1940 period, altering the migration route of outmigrating Pacific lamprey juveniles/larvae and upstream adult migrants (1927-1972). Dworshak Dam, completed in 1972, eliminated Pacific lamprey spawning and rearing in the North Fork Clearwater River drainage. Construction of the Harpster hydroelectric dam on the South Fork of the Clearwater River resulted in obstructed fish passage 1949-1963. Through Bonneville Power Administration support, the Idaho Department of Fish and Game continued investigation into the status of Pacific lamprey populations in Idaho's Clearwater River drainage in 2001. Trapping, electrofishing, and spawning ground redd surveys were used to determine Pacific lamprey distribution, life history strategies, and habitat requirements in the South

  10. Replication fork stability confers chemoresistance in BRCA-deficient cells

    DEFF Research Database (Denmark)

    Chaudhuri, Arnab Ray; Callen, Elsa; Ding, Xia

    2016-01-01

    /4 complex protein, PTIP, protects Brca1/2-deficient cells from DNA damage and rescues the lethality of Brca2-deficient embryonic stem cells. However, PTIP deficiency does not restore homologous recombination activity at double-strand breaks. Instead, its absence inhibits the recruitment of the MRE11......Cells deficient in the Brca1 and Brca2 genes have reduced capacity to repair DNA double-strand breaks by homologous recombination and consequently are hypersensitive to DNA-damaging agents, including cisplatin and poly(ADP-ribose) polymerase (PARP) inhibitors. Here we show that loss of the MLL3...... nuclease to stalled replication forks, which in turn protects nascent DNA strands from extensive degradation. More generally, acquisition of PARP inhibitors and cisplatin resistance is associated with replication fork protection in Brca2-deficient tumour cells that do not develop Brca2 reversion mutations...

  11. A vibrating quartz fork - a tool for cryogenic helium research

    Czech Academy of Sciences Publication Activity Database

    Blažková, Michaela; Člověčko, M.; Eltsov, V. B.; Gažo, E.; de Graaf, R.; Hosio, J.J.; Krusius, M.; Schmoranzer, D.; Schoepe, W.; Skrbek, Ladislav; Skyba, P.; Solntsev, R.E.; Vinen, W. F.

    2008-01-01

    Roč. 150, - (2008), s. 525-535 ISSN 0022-2291 R&D Projects: GA ČR GA202/05/0218 Grant - others:GAUK(CZ) 7953/2007; Transnational Access Programme(XE) RITA -CT-2003-505313 Institutional research plan: CEZ:AV0Z10100520 Keywords : normal 3He * superfluid 3He * superfluid 4He * turbulence, * cavitation * quartz tuning fork Subject RIV: BK - Fluid Dynamics Impact factor: 1.034, year: 2008

  12. Pitch Fork: A Novel tactile Digital Musical Instrument

    OpenAIRE

    Williams, Peter; Overholt, Daniel

    2017-01-01

    Pitch Fork is a prototype of an alternate, actuated digital musical instrument (DMI). It uses 5 infra-red and 4 piezoelectric sensors to control an additive synthesis engine. Iron bars are used as the physical point of contact in interaction with the aim of using material computation to control aspects of the digitally produced sound. This choice of material was also chosen to affect player experience. Sensor readings are relayed to a Macbook via an Arduino Mega. Mappings and audio output sig...

  13. Timing, coordination, and rhythm: Acrobatics at the DNA replication fork

    KAUST Repository

    Hamdan, Samir

    2010-04-09

    In DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

  14. Cell lethality after selective irradiation of the DNA replication fork

    International Nuclear Information System (INIS)

    Hofer, K.G.; Warters, R.L.

    1985-01-01

    It has been suggested that nascent DNA located at the DNA replication fork may exhibit enhanced sensitivity to radiation damage. To evaluate this hypothesis, Chinese hamster ovary cells (CHO) were labeled with 125 I-iododeoxyuridine ( 125 IUdR) either in the presence or absence of aphidicolin. Aphidicolin (5 μg/ml) reduced cellular 125 IUdR incorporation to 3-5% of the control value. The residual 125 I incorporation appeared to be restricted to low molecular weight (sub-replicon sized) fragments of DNA which were more sensitive to micrococcal nuclease attack and less sensitive to high salt DNase I digestion than randomly labeled DNA. These findings suggest that DNA replicated in the presence of aphidicolin remains localized at the replication fork adjacent to the nuclear matrix. Based on these observations an attempt was made to compare the lethal consequences of 125 I decays at the replication fork to that of 125 I decays randomly distributed over the entire genome. Regardless of the distribution of decay events, all treatment groups exhibited identical dose-response curves (D 0 : 101 125 I decays/cell). Since differential irradiation of the replication complex did not result in enhanced cell lethality, it can be concluded that neither the nascent DNA nor the protein components (replicative enzymes, nuclear protein matrix) associated with the DNA replication site constitute key radiosensitive targets within the cellular genome. (orig.)

  15. Timing, coordination, and rhythm: Acrobatics at the DNA replication fork

    KAUST Repository

    Hamdan, Samir; van Oijen, Antoine M.

    2010-01-01

    In DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

  16. Recovery of arrested replication forks by homologous recombination is error-prone.

    Directory of Open Access Journals (Sweden)

    Ismail Iraqui

    Full Text Available Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability. Here, we report that recovery of impeded replication forks by homologous recombination is error-prone. Using a fork-arrest-based assay in fission yeast, we demonstrate that a single collapsed fork can cause mutations and large-scale genomic changes, including deletions and translocations. Fork-arrest-induced gross chromosomal rearrangements are mediated by inappropriate ectopic recombination events at the site of collapsed forks. Inverted repeats near the site of fork collapse stimulate large-scale genomic changes up to 1,500 times over spontaneous events. We also show that the high accuracy of DNA replication during S-phase is impaired by impediments to fork progression, since fork-arrest-induced mutation is due to erroneous DNA synthesis during recovery of replication forks. The mutations caused are small insertions/duplications between short tandem repeats (micro-homology indicative of replication slippage. Our data establish that collapsed forks, but not stalled forks, recovered by homologous recombination are prone to replication slippage. The inaccuracy of DNA synthesis does not rely on PCNA ubiquitination or trans-lesion-synthesis DNA polymerases, and it is not counteracted by mismatch repair. We propose that deletions/insertions, mediated by micro-homology, leading to copy number variations during replication stress may arise by progression of error-prone replication forks restarted by homologous recombination.

  17. Oriented Onion Sowing by a Forked-Roller Sowing Unit

    Directory of Open Access Journals (Sweden)

    Aleksandr G.

    2018-03-01

    Full Text Available Introduction: The existing sowing machines do not provide a single feeding of the bulbs with a planting (sowing unit that leads to a violation of the agrotechnical requirements of planting bulbs. It is necessary to search new solutions to preserve the position of the bulbs in the furrow with the bottom down and their regularly spaced distribution. Materials and Methods: The article presents the design for a prototype for a planting machine equipped with a forked-roller sowing unit for orienting the onion-sowing into a furrow. Testing the forked-roller sowing unit were carried out on a flat area where the physical and mechanical properties of the soil were determined on the days of sowing, and the indices of the quality of the onion-sowing were determined. The study of the effect of the sowing machine speed on the quality of the onion-seed bulb landing was determined by the change in the translational speed of the sowing unit in the range of 0.8 m/s to 1.2 m/s with a variation interval of 0.1 m/s. The indicators of the quality of the planting of the bulbs were determined by the opening of the closed furrow. The results of laboratory-field studies of the planting machine prototype are presented. Results: The results of laboratory-field studies of a planting machine equipped with a forked-roller sowing unit for planting onion bulbs are presented. The optimal technological parameters are determined experimentally. It was determined the number of bulbs that are for up is 51 % and the regularity of planting by the forked-roller sowing unit – 79 %. These figures are provided at the forward speed of the planting machine VM = 0.9–1.0 m/s, the height of the fall of the bulb HA = 0.12 m, and the rotation frequency of the landing drum nБ = 0.47 c-1. Discussion and Conclusions: The use of a forked-roller sowing unit makes it possible to increase the proportion of onions planted by bottom down by 200 %, and the uniformity of planting bulbs by 19 %, in

  18. Take It Slow: can feedback from a smart fork reduce eating speed?

    Directory of Open Access Journals (Sweden)

    Sander Hermsen

    2015-09-01

    The present study examines the efficacy of a smart fork that helps people to eat more slowly. This adapted fork records eating speed and delivers vibrotactile feedback if users eat too quickly. In two studies, we tested the acceptability and user experience of the fork (Study 1, and its effect on eating rate and satiety levels in a controlled lab-setting (Study 2. Method: In study 1, 11 participants (all self-reported fast eaters ate a meal using the fork in our laboratory and used the fork for three consecutive days in their home setting. Participants took part in semi-structured interviews after the first meal and upon returning the fork, covering perceived effect on eating rate, comfort of use, accuracy, and motivational and social aspects of fork use. Interviews were coded and a thematic classification analysis was performed. In study 2, 128 participants (all self-reported fast eaters ate a standardized meal using the fork in our laboratory. We used a between-participants design with 2 conditions; participants ate their meal either with vibrotactile feedback from the fork (experimental condition or ate their meal without vibrotactile feedback (control condition. Eating rate, meal duration, error rate (number of bites taken faster than 10 seconds after previous bite, total food intake, and satiety were recorded for every participant. Results: Study 1: All participants felt that the feedback was generally accurate and consistent. Fork size, weight, and intensity of the feedback were seen as comfortable and acceptable. All participants reported a heightened awareness of eating rate and all but one participant reported eating more slowly with the fork. Study 2: Participants in the experimental condition ate significantly slower, and with a lower error rate than those in the control condition. Feedback did not significantly affect the amount of food eaten. Conclusions: Our findings suggest that this smart fork is an acceptable and effective tool to disrupt and

  19. The fork and the kinase: a DNA replication tale from a CHK1 perspective.

    Science.gov (United States)

    González Besteiro, Marina A; Gottifredi, Vanesa

    2015-01-01

    Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. Checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged DNA. Subsequently, Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Indeed, such findings have unveiled a puzzling connection between Chk1 and DNA lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, Chk1 is a multifaceted and versatile signaling factor that acts at ongoing forks and replication origins to determine the extent and quality of the cellular response to replication stress. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Implementing farm-to-fork traceability in Tanzania

    CSIR Research Space (South Africa)

    Van Dyk, FE

    2005-08-01

    Full Text Available stream_source_info Van Dyk2_2005.pdf.txt stream_content_type text/plain stream_size 10949 Content-Encoding UTF-8 stream_name Van Dyk2_2005.pdf.txt Content-Type text/plain; charset=UTF-8 Copyright @ CSIR 2005 www....csir.co.za Implementing farm-to-fork traceability in Tanzania Esbeth van Dyk CSIR Centre for Logistics ORSSA/SAIIE August 2005 Copyright @ CSIR 2005 www.csir.co.za Structure • Why traceability? • Legislation • Tanzania project • Recordkeeping in coffee...

  1. Burnup measurements with the Los Alamos fork detector

    International Nuclear Information System (INIS)

    Bosler, G.E.; Rinard, P.M.

    1991-01-01

    The fork detector system can determine the burnup of spent-fuel assemblies. It is a transportable instrument that can be mounted permanently in a spent-fuel pond near a loading area for shipping casks, or be attached to the storage pond bridge for measurements on partially raised spent-fuel assemblies. The accuracy of the predicted burnup has been demonstrated to be as good as 2% from measurements on assemblies in the United States and other countries. Instruments have also been developed at other facilities throughout the world using the same or different techniques, but with similar accuracies. 14 refs., 2 figs., 2 tabs

  2. Multiplexing of adjacent vortex modes with the forked grating coupler

    Science.gov (United States)

    Nadovich, Christopher T.; Kosciolek, Derek J.; Crouse, David T.; Jemison, William D.

    2017-08-01

    For vortex fiber multiplexing to reach practical commercial viability, simple silicon photonic interfaces with vortex fiber will be required. These interfaces must support multiplexing. Toward this goal, an efficient singlefed multimode Forked Grating Coupler (FGC) for coupling two different optical vortex OAM charges to or from the TE0 and TE1 rectangular waveguide modes has been developed. A simple, apodized device implemented with e-beam lithography and a conventional dual-etch processing on SOI wafer exhibits low crosstalk and reasonable mode match. Advanced designs using this concept are expected to further improve performance.

  3. Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks

    DEFF Research Database (Denmark)

    Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia

    2016-01-01

    Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose...... RAD52 facilitates repair of collapsed DNA replication forks in cancer cells....

  4. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Science.gov (United States)

    2011-06-20

    ... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

  5. 76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

    Science.gov (United States)

    2011-08-03

    ...-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project Environmental Impact... improve the health of the ecosystem and reach the desired future condition. DATES: Comments concerning the... Ecosystem Restoration Project EIS, P.O. Box 180, 11 Casey Rd., North Fork, ID 83466. Comments may also be...

  6. Both DNA Polymerases δ and ε Contact Active and Stalled Replication Forks Differently

    Science.gov (United States)

    Yu, Chuanhe; Gan, Haiyun

    2017-01-01

    ABSTRACT Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase with active and stalled replication forks. Here, we show that each DNA polymerase has a distinct pattern of association with active and stalled replication forks. Pol α is enriched at extending Okazaki fragments of active and stalled forks. In contrast, although Pol δ contacts the nascent lagging strands of active and stalled forks, it binds to only the matured (and not elongating) Okazaki fragments of stalled forks. Pol ε has greater contact with the nascent single-stranded DNA (ssDNA) of the leading strand on active forks than on stalled forks. We propose that the configuration of DNA polymerases at stalled forks facilitates the resumption of DNA synthesis after stress removal. PMID:28784720

  7. A watershed's response to logging and roads: South Fork of Caspar Creek, California, 1967-1976

    Science.gov (United States)

    Raymond M. Rice; Forest B. Tilley; Patricia A. Datzman

    1979-01-01

    The effect of logging and roadbuilding on erosion and sedimentation are analyzed by comparing the North Fork and South Fork of Caspar Creek, in northern California. Increased sediment production during the 4 years after road construction, was 326 cu yd/sq mi/yr—80 percent greater than that predicted by the predisturbance regression analysis. The average...

  8. Improve performance of scanning probe microscopy by balancing tuning fork prongs

    International Nuclear Information System (INIS)

    Ng, Boon Ping; Zhang Ying; Wei Kok, Shaw; Chai Soh, Yeng

    2009-01-01

    This paper presents an approach for improving the Q-factor of tuning fork probe used in scanning probe microscopes. The improvement is achieved by balancing the fork prongs with extra mass attachment. An analytical model is proposed to characterize the Q-factor of a tuning fork probe with respect to the attachment of extra mass on the tuning fork prongs, and based on the model, the Q-factors of the unbalanced and balanced tuning fork probes are derived and compared. Experimental results showed that the model fits well the experimental data and the approach can improve the Q-factor by more than a factor of three. The effectiveness of the approach is further demonstrated by applying the balanced probe on an atomic force microscope to obtain improved topographic images.

  9. FANCJ couples replication past natural fork barriers with maintenance of chromatin structure.

    Science.gov (United States)

    Schwab, Rebekka A; Nieminuszczy, Jadwiga; Shin-ya, Kazuo; Niedzwiedz, Wojciech

    2013-04-01

    Defective DNA repair causes Fanconi anemia (FA), a rare childhood cancer-predisposing syndrome. At least 15 genes are known to be mutated in FA; however, their role in DNA repair remains unclear. Here, we show that the FANCJ helicase promotes DNA replication in trans by counteracting fork stalling on replication barriers, such as G4 quadruplex structures. Accordingly, stabilization of G4 quadruplexes in ΔFANCJ cells restricts fork movements, uncouples leading- and lagging-strand synthesis and generates small single-stranded DNA gaps behind the fork. Unexpectedly, we also discovered that FANCJ suppresses heterochromatin spreading by coupling fork movement through replication barriers with maintenance of chromatin structure. We propose that FANCJ plays an essential role in counteracting chromatin compaction associated with unscheduled replication fork stalling and restart, and suppresses tumorigenesis, at least partially, in this replication-specific manner.

  10. Quarterly Progress Report - Biological Monitoring Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S.M.; Christensen, S.W.; Greeley, M.S.jr; Hill, W.R.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.

    2000-10-18

    (6) access. The primary sampling sites include upper EFPC at kilometers (EFKs) 24.4 and 23.4 [upstream and downstream of Lake Reality (LR) respectively]; EFK 18.7 (also EFK 18 and 19), located off the ORR and below an area of intensive commercial and light industrial development; EFK 13.8 (also EFK 14), located upstream from the Oak Ridge Wastewater Treatment Facility (ORWTF); and EFK 6.3 located approximately 1.4 km below the ORR boundary (Fig. 1.1). Brushy Fork (BF) at kilometer (BFK) 7.6 is used as a reference stream in most tasks of the BMAP. Additional sites off the ORR are also occasionally used for reference, including Beaver Creek, Bull Run, Hinds Creek, Paint Rock Creek, and the Emory River in Watts Bar Reservoir (Fig. 1.2).

  11. South Fork Flathead Watershed Westslope Cutthroat Trout Conservation Program, Annual Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Grisak, Grant; Marotz, Brian

    2003-06-01

    In 1999, Montana Fish, Wildlife & Parks (MFWP) began a program aimed at conserving the genetically pure populations of westslope cutthroat trout in the South Fork Flathead River drainage. The objective of this program is to eliminate all of the exotic and hybrid trout that threaten the genetically pure westslope cutthroat populations in the South Fork Flathead. The exotic and hybrid trout populations occur in several headwater lakes and their outflow streams. In 2001 MFWP released a draft environmental assessment, pursuant to the Montana Environmental Policy Act (MEPA), that addressed the use of motorized equipment to deliver personnel and materials to some of these lakes in the Bob Marshall and Great Bear Wildernesses (Grisak 2001). After a 30-day public comment period, MFWP determined that the complexity of issues was too great and warranted a more detailed analysis. These issues included transportation options for personnel, equipment and materials, the use of motorized equipment in wilderness, fish removal methods, fish stocking, and the status and distribution of amphibian populations in the project area. Because the program also involves the U.S. Forest Service (USFS) and Bonneville Power Administration (BPA), the environmental analysis needs to comply with the National Environmental Policy Act (NEPA). In October 2001, pursuant to NEPA, MFWP, along with the USFS and BPA initiated an environmental assessment to address these issues. In June 2002, the three agencies determined that the scope of these issues warranted an Environmental Impact Statement. This specialist report describes the logistical, technical and biological issues associated with this project and provides an analysis of options for fish removal, transportation and fish stocking. It further analyzes issues and concerns associated with amphibian populations and creating new domesticated stocks of westslope cutthroat trout. Finally, this document provides a description of each lake, the best

  12. Assessing summer and fall chinook salmon restoration in the Upper Clearwater River and principal tributaries. Annual report 1994

    International Nuclear Information System (INIS)

    Arnsberg, B.D.; Statler, D.P.

    1995-08-01

    This is the first annual report of a five year study to assess summer and fall chinook salmon restoration potential in the upper Clearwater River and principal tributaries, Salmon, Grande Ronde, and Imnaha Rivers. During 1994, the authors focused primarily on assessing water temperatures and spawning habitat in the upper Clearwater River and principal tributaries. Water temperature analysis indicated a colder temperature regime in the upper Clearwater River above the North Fork Clearwater River confluence during the winter as compared to the lower Clearwater. This was due to warm water releases from Dworshak Reservoir on the North Fork moderating temperatures in the lower Clearwater River. Thermal temperature unit analysis and available literature suggest a 75% survival threshold level may be anticipated for chinook salmon egg incubation if spawning would occur by November 1 in the upper Clearwater River. Warm water upwelling in historic summer and fall chinook spawning areas may result in increased incubation survivals and will be tested in the future. The authors observed a total of 37 fall chinook salmon redds in the Clearwater River subbasin. They observed 30 redds in the mainstem Clearwater below the North Fork Clearwater River confluence and seven redds in the North Fork Clearwater River. No redds were observed in the South Fork Clearwater, Middle Fork Clearwater, or Selway Rivers. They observed one fall chinook salmon redd in the Salmon River. They recovered 10 fall chinook salmon carcasses in the Clearwater River to obtain biological measurements and to document hatchery contribution to spawning. Unseasonably high and cold Dworshak Dam releases coinciding with early juvenile fall chinook salmon rearing in the lower Clearwater River may be influencing selective life history traits including growth, smolt development, outmigration timing, behavior, and could be directly affecting survival. During July 1994, discharges from Dworshak Dam increased from a

  13. Groundwater and climate change: a sensitivity analysis for the Grand Forks aquifer, southern British Columbia, Canada

    Science.gov (United States)

    Allen, D. M.; Mackie, D. C.; Wei, M.

    The Grand Forks aquifer, located in south-central British Columbia, Canada was used as a case study area for modeling the sensitivity of an aquifer to changes in recharge and river stage consistent with projected climate-change scenarios for the region. Results suggest that variations in recharge to the aquifer under the different climate-change scenarios, modeled under steady-state conditions, have a much smaller impact on the groundwater system than changes in river-stage elevation of the Kettle and Granby Rivers, which flow through the valley. All simulations showed relatively small changes in the overall configuration of the water table and general direction of groundwater flow. High-recharge and low-recharge simulations resulted in approximately a +0.05 m increase and a -0.025 m decrease, respectively, in water-table elevations throughout the aquifer. Simulated changes in river-stage elevation, to reflect higher-than-peak-flow levels (by 20 and 50%), resulted in average changes in the water-table elevation of 2.72 and 3.45 m, respectively. Simulated changes in river-stage elevation, to reflect lower-than-baseflow levels (by 20 and 50%), resulted in average changes in the water-table elevation of -0.48 and -2.10 m, respectively. Current observed water-table elevations in the valley are consistent with an average river-stage elevation (between current baseflow and peak-flow stages). L'aquifère de Grand Forks, situé en Colombie britannique (Canada), a été utilisé comme zone d'étude pour modéliser la sensibilité d'un aquifère à des modifications de la recharge et du niveau de la rivière, correspondant à des scénarios envisagés de changement climatique dans cette région. Les résultats font apparaître que les variations de recharge de l'aquifère pour différents scénarios de changement climatique, modélisées pour des conditions de régime permanent, ont un impact sur le système aquifère beaucoup plus faible que les changements du niveau des

  14. Data Collection and Simulation of Ecological Habitat and Recreational Habitat in the Shenandoah River, Virginia

    Science.gov (United States)

    Krstolic, Jennifer L.

    2015-01-01

    This report presents updates to methods, describes additional data collected, documents modeling results, and discusses implications from an updated habitat-flow model that can be used to predict ecological habitat for fish and recreational habitat for canoeing on the main stem Shenandoah River in Virginia. Given a 76-percent increase in population predictions for 2040 over 1995 records, increased water-withdrawal scenarios were evaluated to determine the effects on habitat and recreation in the Shenandoah River. Projected water demands for 2040 vary by watershed: the North Fork Shenandoah River shows a 55.9-percent increase, the South Fork Shenandoah River shows a 46.5-percent increase, and the main stem Shenandoah River shows a 52-percent increase; most localities are projected to approach the total permitted surface-water and groundwater withdrawals values by 2040, and a few localities are projected to exceed these values.

  15. Gain-loss study along two streams in the upper Sabine River basin, Texas; August-September 1981

    Science.gov (United States)

    Myers, Dennis R.

    1983-01-01

    A gain-loss study was made August-September 1981 along the upper Sabine River from Lake Tawakoni to Farm Road 2517 near Carthage and along Lake Fork Creek from Lake Fork Reservoir to its junction (mouth) with the Sabine River. The hydrologic data collected during the gain-loss study indicated that during periods of low flow on the Sabine River, at least as much water as is released from Lake Tawakoni and from Lake Fork Reservoir will be available downstream at Farm Road 14 near Big Sandy and at Farm Road 2517 near Carthage. Gains from bank seepage and small tributary inflows compensate for losses due to evaporation, evapotranspiration, and loss of water into the alluvial aquifer.

  16. Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA.

    Science.gov (United States)

    Ripley, Jennifer; Iwanowicz, Luke; Blazer, Vicki; Foran, Christy

    2008-08-01

    The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood cell production in fish, was collected from smallmouth bass at three sites along the Shenandoah River. The tissue was divided for immune function and proteomics analyses. Bactericidal activity and respiratory burst were significantly different between North Fork and mainstem Shenandoah River smallmouth bass, whereas South Fork AK tissue did not significantly differ in either of these measures compared with the other sites. Cytotoxic cell activity was highest among South Fork and lowest among North Fork AK leukocytes. The composite two-dimension gels of the North Fork and mainstem smallmouth bass AK tissues contained 584 and 591 spots, respectively. South Fork smallmouth bass AK expressed only 335 proteins. Nineteen of 50 proteins analyzed by matrix-assisted laser desorption ionization-time of flight were successfully identified. Three of the four identified proteins with increased expression in South Fork AK tissue were involved in metabolism. Seven proteins exclusive to mainstem and North Fork smallmouth bass AK and expressed at comparable abundances serve immune and stress response functions. The proteomics data indicate these fish differ in metabolic capacity of AK tissue and in the ability to produce functional leukocytes. The variable responses of the immune function assays further indicate disruption to the immune system. Our results allow us to hypothesize underlying physiological changes that may relate to fish kills and suggest relevant contaminants known to produce similar physiological disruption.

  17. Salmon River Habitat Enhancement, 1984 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Konopacky, Richard C.

    1986-04-01

    This report has four volumes: a Tribal project annual report (Part 1) and three reports (Parts 2, 3, and 4) prepared for the Tribes by their engineering subcontractor. The Tribal project annual report contains reports for four subprojects within Project 83-359. Subproject I involved habitat and fish inventories in Bear Valley Creek, Valley County, Idaho that will be used to evaluate responses to ongoing habitat enhancement. Subproject II is the coordination/planning activities of the Project Leader in relation to other BPA-funded habitat enhancement projects that have or will occur within the traditional Treaty (Fort Bridger Treaty of 1868) fishing areas of the Shoshone-Bannock Tribes, Fort Hall Reservation, Idaho. Subproject III involved habitat and fish inventories (pretreatment) and habitat problem identification on the Yankee Fork of the Salmon River (including Jordan Creek). Subproject IV during 1985 involved habitat problem identification in the East Fork of the Salmon River and habitat and fish inventories (pretreatment) in Herd Creek, a tributary to the East Fork.

  18. Stalled replication forks generate a distinct mutational signature in yeast

    DEFF Research Database (Denmark)

    Larsen, Nicolai B.; Liberti, Sascha E.; Vogel, Ivan

    2017-01-01

    Proliferating cells acquire genome alterations during the act of DNA replication. This leads to mutation accumulation and somatic cell mosaicism in multicellular organisms, and is also implicated as an underlying cause of aging and tumorigenesis. The molecular mechanisms of DNA replication...... Escherichia coli Tus/Ter complex) engineered into the yeast genome. We demonstrate that transient stalling at this barrier induces a distinct pattern of genome rearrangements in the newly replicated region behind the stalled fork, which primarily consist of localized losses and duplications of DNA sequences....... These genetic alterations arise through the aberrant repair of a single-stranded DNA gap, in a process that is dependent on Exo1- and Shu1-dependent homologous recombination repair (HRR). Furthermore, aberrant processing of HRR intermediates, and elevated HRR-associated mutagenesis, is detectable in a yeast...

  19. The analyst's body as tuning fork: embodied resonance in countertransference.

    Science.gov (United States)

    Stone, Martin

    2006-02-01

    This paper examines the phenomenon of embodied countertransference: where the analyst experiences a somatic reaction rather than the more common countertransference responses of thoughts, feelings, images, fantasies and dreams. Discussion of clinical material considers neurotic and syntonic aspects. The analogy is made of resonance with a tuning fork. Several questions are posed: Why does countertransference resonate in the bodies of some analysts but not all? Why do those analysts who are sensitive to this, experience it with some patients but not with others? And what are the conditions which are conducive to producing somatic responses? It proposes that somatic reactions are more likely to occur when a number of conditions come together: when working with patients exhibiting borderline, psychotic or severe narcissistic elements; where there has been early severe childhood trauma; and where there is fear of expressing strong emotions directly. In addition another theoretical factor is proposed, namely the typology of the analyst.

  20. Recovery of thermophilic Campylobacter by three sampling methods from classified river sites in Northeast Georgia, USA

    Science.gov (United States)

    It is not clear how best to sample streams for the detection of Campylobacter which may be introduced from agricultural or community land use. Fifteen sites in the watershed of the South Fork of the Broad River (SFBR) in Northeastern Georgia, USA, were sampled in three seasons. Seven sites were cl...

  1. Theoretical model and optimization of a novel temperature sensor based on quartz tuning fork resonators

    International Nuclear Information System (INIS)

    Xu Jun; You Bo; Li Xin; Cui Juan

    2007-01-01

    To accurately measure temperatures, a novel temperature sensor based on a quartz tuning fork resonator has been designed. The principle of the quartz tuning fork temperature sensor is that the resonant frequency of the quartz resonator changes with the variation in temperature. This type of tuning fork resonator has been designed with a new doubly rotated cut work at flexural vibration mode as temperature sensor. The characteristics of the temperature sensor were evaluated and the results sufficiently met the target of development for temperature sensor. The theoretical model for temperature sensing has been developed and built. The sensor structure was analysed by finite element method (FEM) and optimized, including tuning fork geometry, tine electrode pattern and the sensor's elements size. The performance curve of output versus measured temperature is given. The results from theoretical analysis and experiments indicate that the sensor's sensitivity can reach 60 ppm 0 C -1 with the measured temperature range varying from 0 to 100 0 C

  2. The Sound Field around a Tuning Fork and the Role of a Resonance Box

    Science.gov (United States)

    Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

    2015-01-01

    Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

  3. Checkpoint-dependent RNR induction promotes fork restart after replicative stress.

    Science.gov (United States)

    Morafraile, Esther C; Diffley, John F X; Tercero, José Antonio; Segurado, Mónica

    2015-01-20

    The checkpoint kinase Rad53 is crucial to regulate DNA replication in the presence of replicative stress. Under conditions that interfere with the progression of replication forks, Rad53 prevents Exo1-dependent fork degradation. However, although EXO1 deletion avoids fork degradation in rad53 mutants, it does not suppress their sensitivity to the ribonucleotide reductase (RNR) inhibitor hydroxyurea (HU). In this case, the inability to restart stalled forks is likely to account for the lethality of rad53 mutant cells after replication blocks. Here we show that Rad53 regulates replication restart through the checkpoint-dependent transcriptional response, and more specifically, through RNR induction. Thus, in addition to preventing fork degradation, Rad53 prevents cell death in the presence of HU by regulating RNR-expression and localization. When RNR is induced in the absence of Exo1 and RNR negative regulators, cell viability of rad53 mutants treated with HU is increased and the ability of replication forks to restart after replicative stress is restored.

  4. Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.

    Science.gov (United States)

    Reynolds, John J; Bicknell, Louise S; Carroll, Paula; Higgs, Martin R; Shaheen, Ranad; Murray, Jennie E; Papadopoulos, Dimitrios K; Leitch, Andrea; Murina, Olga; Tarnauskaitė, Žygimantė; Wessel, Sarah R; Zlatanou, Anastasia; Vernet, Audrey; von Kriegsheim, Alex; Mottram, Rachel M A; Logan, Clare V; Bye, Hannah; Li, Yun; Brean, Alexander; Maddirevula, Sateesh; Challis, Rachel C; Skouloudaki, Kassiani; Almoisheer, Agaadir; Alsaif, Hessa S; Amar, Ariella; Prescott, Natalie J; Bober, Michael B; Duker, Angela; Faqeih, Eissa; Seidahmed, Mohammed Zain; Al Tala, Saeed; Alswaid, Abdulrahman; Ahmed, Saleem; Al-Aama, Jumana Yousuf; Altmüller, Janine; Al Balwi, Mohammed; Brady, Angela F; Chessa, Luciana; Cox, Helen; Fischetto, Rita; Heller, Raoul; Henderson, Bertram D; Hobson, Emma; Nürnberg, Peter; Percin, E Ferda; Peron, Angela; Spaccini, Luigina; Quigley, Alan J; Thakur, Seema; Wise, Carol A; Yoon, Grace; Alnemer, Maha; Tomancak, Pavel; Yigit, Gökhan; Taylor, A Malcolm R; Reijns, Martin A M; Simpson, Michael A; Cortez, David; Alkuraya, Fowzan S; Mathew, Christopher G; Jackson, Andrew P; Stewart, Grant S

    2017-04-01

    To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication and protect, repair and restart damaged forks. Here we identify downstream neighbor of SON (DONSON) as a novel fork protection factor and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilizes forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks. Furthermore, ATM- and Rad3-related (ATR)-dependent signaling in response to replication stress is impaired in DONSON-deficient cells, resulting in decreased checkpoint activity and the potentiation of chromosomal instability. Hypomorphic mutations in DONSON substantially reduce DONSON protein levels and impair fork stability in cells from patients, consistent with defective DNA replication underlying the disease phenotype. In summary, we have identified mutations in DONSON as a common cause of microcephalic dwarfism and established DONSON as a critical replication fork protein required for mammalian DNA replication and genome stability.

  5. Ultrasonic superlensing jets and acoustic-fork sheets

    Energy Technology Data Exchange (ETDEWEB)

    Mitri, F.G., E-mail: F.G.Mitri@ieee.org

    2017-05-18

    Focusing acoustical (and optical) beams beyond the diffraction limit has remained a major challenge in imaging instruments and systems, until recent advances on “hyper” or “super” lensing and higher-resolution imaging techniques have shown the counterintuitive violation of this rule under certain circumstances. Nonetheless, the proposed technologies of super-resolution acoustical focusing beyond the diffraction barrier require complex tools such as artificially engineered metamaterials, and other hardware equipment that may not be easily synthesized or manufactured. The present contribution therefore suggests a simple and reliable method of using a sound-penetrable circular cylinder lens illuminated by a nonparaxial Gaussian acoustical sheet (i.e. finite beam in 2D) to produce non-evanescent ultrasonic superlensing jets (or bullets) and acoustical ‘snail-fork’ shaped wavefronts with limited diffraction. The generalized (near-field) scattering theory for acoustical sheets of arbitrary wavefronts and incidence is utilized to synthesize the incident beam based upon the angular spectrum decomposition method and the multipole expansion method in cylindrical wave functions to compute the scattered pressure around the cylinder with particular emphasis on its physical properties. The results show that depending on the beam and lens parameters, a tight focusing (with dimensions much smaller than the beam waist) can be achieved. Subwavelength resolution can be also achieved by selecting a lens material with a speed of sound exceeding that of the host fluid medium. The ultrasonic superlensing jets provide the impetus to develop improved subwavelength microscopy and acoustical image-slicing systems, cell lysis and surgery, and photoacoustic imaging to name a few examples. Moreover, an acoustical fork-sheet generation may open innovative avenues in reconfigurable on-chip micro/nanoparticle tweezers and surface acoustic waves devices. - Highlights: • Ultrasonic

  6. Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

    Science.gov (United States)

    Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

    2015-06-01

    A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever.

  7. Soil Investigation of Lower East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Dickson, Johnbull O [ORNL; Mayes, Melanie [ORNL; Earles, Jennifer E [ORNL; Mehlhorn, Tonia L [ORNL; Lowe, Kenneth Alan [ORNL; Peterson, Mark J [ORNL; Pierce, Eric M [ORNL

    2017-03-01

    Mercury is regarded by the US Department of Energy (DOE) Oak Ridge Office of Environmental Management as a priority contaminant on the Oak Ridge Reservation because of the environmental risks associated with substantial losses from buildings, soils, and surface waters at the Y-12 National Security Complex (Y-12). As a result of historical releases of mercury from Y-12 primarily in the 1950s and early 1960s, the lower East Fork Poplar Creek (LEFPC) stream channel and bank soil margins are contaminated with mercury (Brooks and Southworth 2011; Tennessee Valley Authority 1985b, a). A Mercury Remediation Technology Development project is underway to evaluate the nature of downstream mercury contamination and to develop targeted site-specific remedial technologies that can mitigate mercury release and biological uptake. It is known that mercury concentration varies longitudinally and with depth in LEFPC bank soils; however, soil types and soil physical properties are not well known, especially relative to the zones of mercury contamination. Moreover, there are no soil maps for the downstream reaches of LEFPC in Roane County (i.e. from the Chestnut Hill Road downstream) and this work represents the first ever soil mapping along this section of LEFPC.

  8. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    Energy Technology Data Exchange (ETDEWEB)

    ADAMS, S.M.; ASHWOOD, T.L.; BEATY, T.W.; BRANDT, C.C.

    1997-10-24

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y- 12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  9. Biological monitoring program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S.M.; Ashwood, T.L.; Beaty, T.W.; Brandt, C.C.; Christensen, S.W.; Cicerone, D.S.; Greeley, M.S. Jr.; Hill, W.R.; Kszos, L.S.

    1997-04-18

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  10. Tuning Fork Oscillators as Downhole Viscometers in Oilfield Applications

    Science.gov (United States)

    Gonzalez, Miguel; Bernero, Greg; Alvarez, Oliverio; Ham, Gregory; Max, Deffenbaugh; Sensors Development Team

    2015-03-01

    The commerciality of oil wells is greatly influenced by the physical properties of the fluids being produced. A key parameter in determining how producible the hydrocarbons are is their viscosity. Pressure and temperature changes in recovering a downhole sample to the surface can alter viscosity while accurate downhole measurement of this critical property remains a rudimentary effort in the industry. In this presentation we describe the challenges of measuring and quantifying the viscosity of reservoir fluids in situ at downhole conditions, as well as present an overview of some of the different measurement techniques currently used. Additionally, we show our characterization of a piezoelectric tuning fork oscillator used as a viscosity sensor. In an attempt to recreate the environment found in oil wells, its mechanical and electrical properties were studied while the device was immersed in different fluids and, separately, under different conditions of pressure and temperature. This device is a first step toward the development of an inexpensive, integrated, and miniaturized sensing platform for the in situ characterization of reservoir fluids.

  11. Bioavailability of mercury in East Fork Poplar Creek soils

    International Nuclear Information System (INIS)

    Barnett, M.O.; Turner, R.R.

    1995-05-01

    The initial risk assessment for the East Fork Poplar Creek (EFPC) floodplain in Oak Ridge, Tennessee, a superfund site heavily contaminated with mercury, was based upon a reference dose for mercuric chloride, a soluble mercury compound not expected to be present in the floodplain, which is frequently saturated with water. Previous investigations had suggested mercury in the EFPC floodplain was less soluble and therefore less bioavailable than mercuric chloride, possibly making the results of the risk assessment unduly conservative. A bioavailability study, designed to measure the amount of mercury available for absorption in a child's digestive tract, the most critical risk endpoint and pathway, was performed on twenty soils from the EFPC floodplain. The average percentage of mercury released during the study for the twenty soils was 5.3%, compared to 100% of the compound mercuric chloride subjected to the same conditions. Alteration of the procedure to test additional conditions possible during soil digestion did not appreciably alter the results. Therefore, use of a reference dose for mercuric chloride in the EFPC risk assessment without inclusion of a corresponding bioavailability factor may be unduly conservative

  12. Failure analysis of axle shaft of a fork lift

    Directory of Open Access Journals (Sweden)

    Souvik Das

    2015-04-01

    Full Text Available An axle shaft of fork lift failed at operation within 296 h of service. The shaft transmits torque from discrepancy to wheel through planetary gear arrangement. A section of fractured axle shaft made of induction-hardened steel was analyzed to determine the root cause of the failure. Optical microscopies as well as field emission gun scanning electron microscopy (FEG-SEM along with energy dispersive spectroscopy (EDS were carried out to characterize the microstructure. Hardness profile throughout the cross-section was evaluated by micro-hardness measurements. Chemical analysis indicated that the shaft was made of 42CrMo4 steel grade as per specification. Microstructural analysis and micro-hardness profile revealed that the shaft was improperly heat treated resulting in a brittle case, where crack was found to initiate from the case in a brittle mode in contrast to ductile mode within the core. This behaviour was related to differences in microstructure, which was observed to be martensitic within the case with a micro-hardness equivalent to 735 HV, and a mixture of non-homogeneous structure of pearlite and ferrite within the core with a hardness of 210 HV. The analysis suggests that the fracture initiated from the martensitic case as brittle mode due to improper heat treatment process (high hardness. Moreover the inclusions along the hot working direction i.e. in the longitudinal axis made the component more susceptible to failure.

  13. Intestinal Fork Head Regulates Nutrient Absorption and Promotes Longevity

    Directory of Open Access Journals (Sweden)

    Ekin Bolukbasi

    2017-10-01

    Full Text Available Reduced activity of nutrient-sensing signaling networks can extend organismal lifespan, yet the underlying biology remains unclear. We show that the anti-aging effects of rapamycin and reduced intestinal insulin/insulin growth factor (IGF signaling (IIS require the Drosophila FoxA transcription factor homolog Fork Head (FKH. Intestinal FKH induction extends lifespan, highlighting a role for the gut. FKH binds to and is phosphorylated by AKT and Target of Rapamycin. Gut-specific FKH upregulation improves gut barrier function in aged flies. Additionally, it increases the expression of nutrient transporters, as does lowered IIS. Evolutionary conservation of this effect of lowered IIS is suggested by the upregulation of related nutrient transporters in insulin receptor substrate 1 knockout mouse intestine. Our study highlights a critical role played by FKH in the gut in mediating anti-aging effects of reduced IIS. Malnutrition caused by poor intestinal absorption is a major problem in the elderly, and a better understanding of the mechanisms involved will have important therapeutic implications for human aging.

  14. Final Environmental Assessment: Installation of Digital Airport Surveillance Radar at Grand Forks Air Force Base, North Dakota

    Science.gov (United States)

    2011-05-11

    Grand Forks AFB public web site. Notices of Availability were published in the Grand Forks Herald on 10 Mar 2011 and on the Grand Forks AFB web site from...Squadron (319th) FTA Fire Training Area GATR Ground-Air Transmit Receive GFAFB Grand Forks Air Force Base GHG Greenhouse Gas Hz Hertz IEEE Institute of...feet west of the closed/capped ERP Site FT-02, the Fire Training Area/Old Sanitary Landfill Area ( FTA /OSLA), which encompasses 28 acres, five of

  15. Lower Red River Meadow Stream Restoration Project

    International Nuclear Information System (INIS)

    1996-05-01

    As part of a continuing effort to restore anadromous fish populations in the South Fork Clearwater River basin of Idaho, Bonneville Power Administration (BPA) proposes to fund the Lower Red River Meadow Restoration Project (Project). The Project is a cooperative effort with the Idaho Soil and Water Conservation District, Nez Perce National Forest, Idaho Department of Fish and Game (IDFG), and the Nez Perce Tribe of Idaho. The proposed action would allow the sponsors to perform stream bank stabilization, aquatic and riparian habitat improvement activities on IDFG's Red River Management Area and to secure long-term conservation contracts or agreements for conducting streambank and habitat improvement activities with participating private landowners located in the Idaho County, Idaho, study area. This preliminary Environmental Assessment (EA) examines the potential environmental effects of stabilizing the stream channel, restoring juvenile fish rearing habitat and reestablishing a riparian shrub community along the stream

  16. Quarterly Progress Report - Biological Monitoring Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S. M.; Christensen, S. W.; Greeley, M.S. jr; McCracken, M.K.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth G. R.; Stewart, A. J.

    2001-01-19

    adjacent floodplain, (5) appropriate habitat distribution, and (6) access. The primary sampling sites include upper EFPC at kilometers (EFKs) 24.4 and 23.4 [upstream and downstream of Lake Reality (LR) respectively]; EFK 18.7 (also EFK 18 and 19), located off the ORR and below an area of intensive commercial and light industrial development; EFK 13.8 (also EFK 14), located upstream from the Oak Ridge Wastewater Treatment Facility (ORWTF); and EFK 6.3 located approximately 1.4 km below the ORR boundary (Fig. 1.1). Brushy Fork (BF) at kilometer (BFK) 7.6 is used as a reference stream in most tasks of the BMAP. Additional sites off the ORR are also occasionally used for reference, including Beaver Creek, Bull Run, Hinds Creek, Paint Rock Creek, and the Emory River in Watts Bar Reservoir (Fig. 1.2).

  17. Predicting Fecal Indicator Bacteria Concentrations in the South Fork Broad River Watershed Using Virtual Beach

    Science.gov (United States)

    Virtual Beach (VB) is a decision support tool that constructs site-specific statistical models to predict fecal indicator bacteria (FIB) at recreational beaches. Although primarily designed for making decisions regarding beach closures or issuance of swimming advisories based on...

  18. 77 FR 66541 - Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham, AL

    Science.gov (United States)

    2012-11-06

    ... authority under section 4(a) of the Administrative Procedure Act (APA) (5 U.S.C. 553(b)). This provision... Department of Homeland Security Management Directive 023-01 and Commandant Instruction M16475.lD, which guide...

  19. Foundation Report on Stonewall Jackson Dam, West Fork River Basin, Weston, West Virginia. Volume 1.

    Science.gov (United States)

    1987-12-21

    Sprague arid Heriwcood -, kid t ig , onto thie hiop Iaid core drtill to required depth. 1he core -,amflpIes wet e obta inied by using a double t The, 4 by 5...Tape GHS 4 seismograph was provided by Mr. Dale R. Fuck on June 2, 1982, at your Roanoke office. The followin; people were present for that...34 .. such dwsnge may b. -~do by tourva ar lowe. p,...dd mo" mi.um.i or "a nah". -fom.. to the sdeli s..e o s kid *anU fW wad is r.ceired prie IS ie. qpq..q

  20. Uintah County Hydrology Report for Detailed Studies on the American Fork River

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic procedures for estimating flood discharges for a flood insurance...

  1. South Fork of the Santa Clara River, Santa Clarita Valley, California. Supplement.

    Science.gov (United States)

    1985-01-01

    designed as a broad - crested weir to pass the maximum probable flood (PF) with a peak of 24,500 ofs. The spillway crest length of 100 feet and the...well as from the Miocene Topanga sandstone and the Modelo shale found near the crest of the Santa Susana Mountains in Aliso and Rice Canyons. Fossil...440 1.5 .IN THE AREA. 1I0.050: DRAINAGE AREA IS QUITE RUGGED, WITH SHARP RIDGES 4.4 600 1.3 .050 AND NARROW, STEEP CANYONS THROUGH WHICH WATERCOURSES

  2. Cultural Resource Investigation of the Dworshak Reservoir Project, North Fork Clearwater River, Northern Idaho.

    Science.gov (United States)

    1983-01-01

    8217;ubp hda ,. ,fl ,in:;. lanceolate and large side-notched points in almost equal amounts. The Tucannon Phase (5000-2500 BP) assemblages of the Lower Snake...Copper kettles must be coated with tin to prevent a poisonous chemical reaction when acidic foods are cooked in the kettle (Stapp 1980c, n.d.). The...dark, organic, greasy) only 5: Fibrous organic substance only 6: Pigment plus some organic material 0: Indeterminate (as if acid -bathed, burned, etc

  3. Predicting E. Coli and Enterococci Concentrations in the South Fork Broad River Watershed Using Virtual Beach

    Science.gov (United States)

    Virtual Beach (VB) is a decision support tool that constructs site-specific statistical models to predict fecal indicator bacteria (FIB) at locations of exposure. Although primarily designed for making decisions regarding beach closures or issuance of swimming advisories based on...

  4. An Archeological Survey in the Gypsum Breaks on the Elm Fork of the Red River,

    Science.gov (United States)

    1979-01-01

    Pumpkin Creek in Love County, Oklahoma. The projectile points resemble Meserve, Milnesand, Plainview, Plainview golondrina, Scottsbluff, in addition to...in this survey corresponds to the criteria or factors proposed by Bell and Gettys (n.d.). 1. Size-Area of occupation 7. Degree of preservation 2. Depth...Archeological Survey. Norman. Wyckoff, Don G. and Lyonel Taylor 1971 The Pumpkin Creek Site: An Early Archaic Camp on the Southern Plains Border. Plains

  5. EPA Region 10 Climate Change and TMDL Pilot – South Fork Nooksack River, Washington Final Report

    Science.gov (United States)

    This final report provides an overarching summary of the pilot research project, including the methods used in and the findings of the quantitative and qualitative assessments. It is written to appeal to a wide audience of policy makers, managers, agency staff and the general pub...

  6. DNA Copy-Number Control through Inhibition of Replication Fork Progression

    Directory of Open Access Journals (Sweden)

    Jared T. Nordman

    2014-11-01

    Full Text Available Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell-cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, specific regions of the genome are repressed for DNA replication during differentiation by the SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate that SUUR is recruited to active replication forks and mediates the repression of DNA replication by directly inhibiting replication fork progression instead of functioning as a replication fork barrier. Mass spectrometry identification of SUUR-associated proteins identified the replicative helicase member CDC45 as a SUUR-associated protein, supporting a role for SUUR directly at replication forks. Our results reveal that control of eukaryotic DNA copy number can occur through the inhibition of replication fork progression.

  7. Mechanically stable tuning fork sensor with high quality factor for the atomic force microscope.

    Science.gov (United States)

    Kim, Kwangyoon; Park, Jun-Young; Kim, K B; Lee, Naesung; Seo, Yongho

    2014-01-01

    A quartz tuning fork was used instead of cantilever as a force sensor for the atomic force microscope. A tungsten tip was made by electrochemical etching from a wire of 50 µm diameter. In order to have mechanical stability of the tuning fork, it was attached on an alumina plate. The tungsten tip was attached on the inside end of a prong of a tuning fork. The phase shift was used as a feedback signal to control the distance between the tip and sample, and the amplitude was kept constant using a lock-in amplifier and a homemade automatic gain controller. Due to the mechanical stability, the sensor shows a high quality factor (∼10(3)), and the image quality obtained with this sensor was equivalent to that of the cantilever-based AFM. © 2014 Wiley Periodicals, Inc.

  8. The Coefficient of the Voltage Induced Frequency Shift Measurement on a Quartz Tuning Fork

    Directory of Open Access Journals (Sweden)

    Yubin Hou

    2014-11-01

    Full Text Available We have measured the coefficient of the voltage induced frequency shift (VIFS of a 32.768 KHz quartz tuning fork. Three vibration modes were studied: one prong oscillating, two prongs oscillating in the same direction, and two prongs oscillating in opposite directions. They all showed a parabolic dependence of the eigen-frequency shift on the bias voltage applied across the fork, due to the voltage-induced internal stress, which varies as the fork oscillates. The average coefficient of the VIFS effect is as low as several hundred nano-Hz per millivolt, implying that fast-response voltage-controlled oscillators and phase-locked loops with nano-Hz resolution can be built.

  9. Chemical bond imaging using higher eigenmodes of tuning fork sensors in atomic force microscopy

    Science.gov (United States)

    Ebeling, Daniel; Zhong, Qigang; Ahles, Sebastian; Chi, Lifeng; Wegner, Hermann A.; Schirmeisen, André

    2017-05-01

    We demonstrate the ability of resolving the chemical structure of single organic molecules using non-contact atomic force microscopy with higher normal eigenmodes of quartz tuning fork sensors. In order to achieve submolecular resolution, CO-functionalized tips at low temperatures are used. The tuning fork sensors are operated in ultrahigh vacuum in the frequency modulation mode by exciting either their first or second eigenmode. Despite the high effective spring constant of the second eigenmode (on the order of several tens of kN/m), the force sensitivity is sufficiently high to achieve atomic resolution above the organic molecules. This is observed for two different tuning fork sensors with different tip geometries (small tip vs. large tip). These results represent an important step towards resolving the chemical structure of single molecules with multifrequency atomic force microscopy techniques where two or more eigenmodes are driven simultaneously.

  10. Sediment transport and storage in North Fork Caspar Creek, Mendocino County, California: water years 1980-1988

    Science.gov (United States)

    Michael Brent Napolitano

    1996-01-01

    Abstract - The old-growth redwood forest of North Fork Caspar Creek was clear-cut between 1864 and 1904. Previous research on logging-related changes in suspended sediment and streamflow would suggest that North Fork Caspar Creek has recovered from historical logging (Rice et al., 1979; Ziemer, 1981); research on the influence of large woody debris (LWD) on channel...

  11. Biogeochemistry of mercury in a river-reservoir system: impact of an inactive chloralkali plant on the Holston River-Cherokee Reservoir, Virginia and Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Hildebrand, S. G.; Lindberg, S. E.; Turner, R. R.; Huckabee, J. W.; Strand, R. H.; Lund, J. R.; Andren, A. W.

    1980-08-01

    Elevated mercury concentrations in fish species from the North Fork of the Holston River were observed in the early 1970's. The source of the mercury was a chloralkali plant which had ceased operation in 1972. Mercury continues to be released to the river from two large (approx. 40-ha) waste disposal ponds at the plant site. This report presents results of a study of the emission of mercury to the environment from the abandoned waste ponds and of the distribution of mercury in water, sediment, and biota of the Holston River-Cherokee Reservoir System in Virginia and eastern Tennessee.

  12. Reduced fuel consumption for fork-lift trucks with hydrostatic transmission

    Energy Technology Data Exchange (ETDEWEB)

    Abels, T

    1983-05-01

    Cost calculations for a 3,5-t diesel fork lifter done on the basis of VDI 2695 shows, that fuel costs account only for a small part of the operating costs despite the price increase for diesel fuel. Fork lifters with disk-cam controlled primary/secondary adjusted hydrostatic transmission used less fuel than was indicated in the VDI-guideline. Fuel consumption could further be reduced by an optimized hydraulic adjustment together with a precisely harmonized engine speed adjustment. Annual cost savings are considerable.

  13. Burnup verification tests with the FORK measurement system-implementation for burnup credit

    International Nuclear Information System (INIS)

    Ewing, R.I.

    1994-01-01

    Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. It was designed at Los Alamos National Laboratory for the International Atomic Energy Agency safeguards program and is well suited to verify burnup and cooling time records at commercial Pressurized Water Reactor (PWR) sites. This report deals with the application of the FORK system to burnup credit operations

  14. River engineering

    NARCIS (Netherlands)

    De Vries, M.

    1993-01-01

    One dimension models - basic eauations, analytical models, numberical models. One dimensional models -suspended load, roughness and resistance of river beds. Solving river problems - tools, flood mitigation, bank protection.

  15. Plant composition in oak savanna and woodland restoration at Prairie Fork Conservation Area in Missouri

    Science.gov (United States)

    Nadia E. Navarrete-Tindall; J.W. Van Sambeek; Jamie Coe; Warren Taylor

    2007-01-01

    The wooded areas of the Prairie Fork Conservation Area in central Missouri are typical of the oak/hickory forest/prairie transition zone that will require active management to restore pre-settlement, grass dominated savannas and open woodlands to improve habitat for wildlife. We initiated a management program to restore savannas and woodlands by reducing the midstory (...

  16. 76 FR 6114 - Lincoln National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization

    Science.gov (United States)

    2011-02-03

    ... National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization AGENCY: Forest Service, USDA. ACTION: Notice of Intent to prepare an Environmental Impact Statement. SUMMARY: The Lincoln National Forest will prepare an Environmental Impact Statement (EIS) to document and publicly disclose...

  17. Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function.

    Science.gov (United States)

    Dijkstra, Anke A; Lin, Li-Chun; Nana, Alissa L; Gaus, Stephanie E; Seeley, William W

    2018-01-01

    The human anterior cingulate and frontoinsular cortices are distinguished by 2 unique Layer 5 neuronal morphotypes, the von Economo neurons (VENs) and fork cells, whose biological identity remains mysterious. Insights could impact research on diverse neuropsychiatric diseases to which these cells have been linked. Here, we leveraged the Allen Brain Atlas to evaluate mRNA expression of 176 neurotransmitter-related genes and identified vesicular monoamine transporter 2 (VMAT2), gamma-aminobutyric acid (GABA) receptor subunit θ (GABRQ), and adrenoreceptor α-1A (ADRA1A) expression in human VENs, fork cells, and a minority of neighboring Layer 5 neurons. We confirmed these results using immunohistochemistry or in situ hybridization. VMAT2 and GABRQ expression was absent in mouse cerebral cortex. Although VMAT2 is known to package monoamines into synaptic vesicles, in VENs and fork cells its expression occurs in the absence of monoamine-synthesizing enzymes or reuptake transporters. Thus, VENs and fork cells may possess a novel, uncharacterized mode of cortical monoaminergic function that distinguishes them from most other mammalian Layer 5 neurons. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. The progression of replication forks at natural replication barriers in live bacteria

    NARCIS (Netherlands)

    Moolman, M.C.; Tiruvadi Krishnan, S; Kerssemakers, J.W.J.; de Leeuw, R.; Lorent, V.J.F.; Sherratt, David J.; Dekker, N.H.

    2016-01-01

    Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these

  19. Performance Evaluation of the New Fork-Absorbers of RSG-GAS Control Rod

    International Nuclear Information System (INIS)

    Slamet Wiranto; Purwadi; Arif Hidayat; Agus Sanjaya

    2012-01-01

    During the operation of RSG-GAS reactor, it has been replaced 8 fork-absorber by the new absorber from PT. Batan Teknologi. After almost 5 years under utilization it is important to be evaluated to determine the physical condition and its performance, which is still in good condition and functioning according to the requirements of its operations. The evaluation has been carried out by studying and analyzing the data of the fork-absorber utilization in the the reactor core. The fork absorber data consist of visual inspection, control rod drop time measurement and control rod reactivity and safety margin measurement for each operation cycle. Through the observation up to date with the operating cycle of 79, could be concluded that the fork-absorber condition is still good, and has ability, to support the operation until ± 660 MWD/cycle, which is characterized by obtaining the value of ρ-excess is sufficient for operation, with a large safety margin. (author)

  20. "Forked Tongue: The Politics of Bilingual Education," by Rosalie Pedalino Porter. Book Review.

    Science.gov (United States)

    Amabisca, Anastasia Aimee

    1994-01-01

    Based on personal experience rather than empirical evidence, "Forked Tongue" presents a biased view that could damage the future of bilingual education in the United States. The book considers bilingual education a threat because it prolongs the learning of English and promotes the maintenance of other "un-American" languages…

  1. Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.

    Science.gov (United States)

    Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia; Evangelou, Konstantinos; Da-Ré, Caterina; Huber, Florian; Padayachy, Laura; Tardy, Sebastien; Nicati, Noemie L; Barriot, Samia; Ochs, Fena; Lukas, Claudia; Lukas, Jiri; Gorgoulis, Vassilis G; Scapozza, Leonardo; Halazonetis, Thanos D

    2016-12-15

    Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose depletion inhibited G1 to S phase progression when oncogenic cyclin E was overexpressed. RAD52, a gene dispensable for normal development in mice, was among the top hits. In cells in which fork collapse was induced by oncogenes or chemicals, the Rad52 protein localized to DRS foci. Depletion of Rad52 by siRNA or knockout of the gene by CRISPR/Cas9 compromised restart of collapsed forks and led to DNA damage in cells experiencing DRS. Furthermore, in cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan. We therefore propose that mammalian RAD52 facilitates repair of collapsed DNA replication forks in cancer cells. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  2. Dual parasitism of Fork-tailed Drongos by African and Jacobin ...

    African Journals Online (AJOL)

    Different species of brood parasitic birds, which lay their eggs in the nests of host foster-parents, rarely target the same host species population. We report brood parasitism of Fork-tailed Drongos Dicrurus adsimilis in the southern Kalahari Desert by both African Cuckoo Cuculus gularis and Jacobin Cuckoo Clamator ...

  3. Effects of timber harvest on aquatic vertebrates and habitat in the North Fork Caspar Creek

    Science.gov (United States)

    Rodney J. Nakamoto

    1998-01-01

    I examined the relationships between timber harvest, creek habitat, and vertebrate populations in the North and South forks of Caspar Creek. Habitat inventories suggested pool availability increased after the onset of timber harvest activities. Increased large woody debris in the channel was associated with an increase in the frequency of blowdown in the riparian...

  4. Storm water control plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This document provides the Environmental Restoration Program with information about the erosion and sediment control, storm water management, maintenance, and reporting and record keeping practices to be employed during Phase II of the remediation project for the Lower East Fork Poplar Creek (LEFPC) Operable Unit

  5. 76 FR 20463 - Endangered and Threatened Wildlife and Plants; Proposed Endangered Status for the Three Forks...

    Science.gov (United States)

    2011-04-12

    ... precariously, at Three Forks Springs. Lack of vegetation and forest litter following intense crown fires can... Policy and Directives Management; U.S. Fish and Wildlife Service; 4401 N. Fairfax Drive, Suite 222; Arlington, VA 22203. We will not accept e-mail or faxes. We will post all comments on http://www.regulations...

  6. Noncontact atomic force microscopy in liquid environment with quartz tuning fork and carbon nanotube probe

    DEFF Research Database (Denmark)

    Kageshima, M.; Jensenius, Henriette; Dienwiebel, M.

    2002-01-01

    A force sensor for noncontact atomic force microscopy in liquid environment was developed by combining a multiwalled carbon nanotube (MWNT) probe with a quartz tuning fork. Solvation shells of octamethylcyclotetrasiloxane surface were detected both in the frequency shift and dissipation. Due to t...

  7. Geomorphic changes resulting from floods in reconfigured gravel-bed river channels in Colorado, USA

    Science.gov (United States)

    Elliott, J.G.; Capesius, J.P.

    2009-01-01

    Geomorphic changes in reconfi gured reaches of three Colorado rivers in response to floods in 2005 provide a benchmark for "restoration" assessment. Sedimententrainment potential is expressed as the ratio of the shear stress from the 2 yr, 5 yr, 10 yr, and 2005 floods to the critical shear stress for sediment. Some observed response was explained by the excess of flood shear stress relative to the resisting force of the sediment. Bed-load entrainment in the Uncompahgre River and the North Fork Gunnison River, during 4 and 6 yr floods respectively, resulted in streambed scour, streambed deposition, lateral-bar accretion, and channel migration at various locations. Some constructed boulder and log structures failed because of high rates of bank erosion or bed-material deposition. The Lake Fork showed little or no net change after the 2005 flood; however, this channel had not conveyed floods greater than the 2.5 yr flood since reconfi guration. Channel slope and the 2 yr flood, a surrogate for bankfull discharge, from all three reconfi gured reaches plotted above the Leopold and Wolman channel-pattern threshold in the "braided channel" region, indicating that braiding, rather than a single-thread meandering channel, and midchannel bar formation may be the natural tendency of these gravel-bed reaches. When plotted against a total stream-power and median-sediment-size threshold for the 2 yr flood, however, the Lake Fork plotted in the "single-thread channel" region, the North Fork Gunnison plotted in the " multiplethread" region, and the Uncompahgre River plotted on the threshold. All three rivers plotted in the multiple-thread region for floods of 5 yr recurrence or greater. ?? 2009 Geological Society of America.

  8. High-affinity DNA-binding Domains of Replication Protein A (RPA) Direct SMARCAL1-dependent Replication Fork Remodeling*

    Science.gov (United States)

    Bhat, Kamakoti P.; Bétous, Rémy; Cortez, David

    2015-01-01

    SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. PMID:25552480

  9. High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling.

    Science.gov (United States)

    Bhat, Kamakoti P; Bétous, Rémy; Cortez, David

    2015-02-13

    SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  10. Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.

    Science.gov (United States)

    Hilton, Benjamin A; Liu, Ji; Cartwright, Brian M; Liu, Yiyong; Breitman, Maya; Wang, Youjie; Jones, Rowdy; Tang, Hui; Rusinol, Antonio; Musich, Phillip R; Zou, Yue

    2017-09-01

    Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder that is caused by a point mutation in the LMNA gene, resulting in production of a truncated farnesylated-prelamin A protein (progerin). We previously reported that XPA mislocalized to the progerin-induced DNA double-strand break (DSB) sites, blocking DSB repair, which led to DSB accumulation, DNA damage responses, and early replication arrest in HGPS. In this study, the XPA mislocalization to DSBs occurred at stalled or collapsed replication forks, concurrent with a significant loss of PCNA at the forks, whereas PCNA efficiently bound to progerin. This PCNA sequestration likely exposed ds-ssDNA junctions at replication forks for XPA binding. Depletion of XPA or progerin each significantly restored PCNA at replication forks. Our results suggest that although PCNA is much more competitive than XPA in binding replication forks, PCNA sequestration by progerin may shift the equilibrium to favor XPA binding. Furthermore, we demonstrated that progerin-induced apoptosis could be rescued by XPA, suggesting that XPA-replication fork binding may prevent apoptosis in HGPS cells. Our results propose a mechanism for progerin-induced genome instability and accelerated replicative senescence in HGPS.-Hilton, B. A., Liu, J., Cartwright, B. M., Liu, Y., Breitman, M., Wang, Y., Jones, R., Tang, H., Rusinol, A., Musich, P. R., Zou, Y. Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes. © FASEB.

  11. Simple force balance accelerometer/seismometer based on a tuning fork displacement sensor

    International Nuclear Information System (INIS)

    Stuart-Watson, D.; Tapson, J.

    2004-01-01

    Seismometers and microelectromechanical system accelerometers use the force-balance principle to obtain measurements. In these instruments the displacement of a mass object by an unknown force is sensed using a very high-resolution displacement sensor. The position of the object is then stabilized by applying an equal and opposite force to it. The magnitude of the stabilizing force is easily measured, and is assumed to be equivalent to the unknown force. These systems are critically dependent on the displacement sensor. In this article we use a resonant quartz tuning fork as the sensor. The tuning fork is operated so that its oscillation is lightly damped by the proximity of the movable mass object. Changes in the position of the mass object cause changes in the phase of the fork's resonance; this is used as the feedback variable in controlling the mass position. We have developed an acceleration sensor using this principle. The mass object is a piezoelectric bimorph diaphragm which is anchored around its perimeter, allowing direct electronic control of the displacement of its center. The tuning fork is brought very close to the diaphragm center, and is connected into a self-oscillating feedback circuit which has phase and amplitude as outputs. The diaphragm position is adjusted by a feedback loop, using phase as the feedback variable, to keep it in a constant position with respect to the tuning fork. The measured noise for this sensor is approximately 10.0 mg in a bandwidth of 100 Hz, which is substantially better than commercial systems of equivalent cost and size

  12. Monitoring and Evaluation of Environmental Flow Prescriptions for Five Demonstration Sites of the Sustainable Rivers Project

    Science.gov (United States)

    Konrad, Christopher P.

    2010-01-01

    The Nature Conservancy has been working with U.S. Army Corps of Engineers (Corps) through the Sustainable Rivers Project (SRP) to modify operations of dams to achieve ecological objectives in addition to meeting the authorized purposes of the dams. Modifications to dam operations are specified in terms of environmental flow prescriptions that quantify the magnitude, duration, frequency, and seasonal timing of releases to achieve specific ecological outcomes. Outcomes of environmental flow prescriptions implemented from 2002 to 2008 have been monitored and evaluated at demonstration sites in five rivers: Green River, Kentucky; Savannah River, Georgia/South Carolina; Bill Williams River, Arizona; Big Cypress Creek, Texas; and Middle Fork Willamette River, Oregon. Monitoring and evaluation have been accomplished through collaborative partnerships of federal and state agencies, universities, and nongovernmental organizations.

  13. Evaluation of Lower East Fork Poplar Creek Mercury Sources

    International Nuclear Information System (INIS)

    Watson, David B.; Brooks, Scott C.; Mathews, Teresa J.; Bevelhimer, Mark S.; DeRolph, Chris; Brandt, Craig C.; Peterson, Mark J.; Ketelle, Richard

    2016-01-01

    This report summarizes a 3-year research project undertaken to better understand the nature and magnitude of mercury (Hg) fluxes in East Fork Poplar Creek (EFPC). This project addresses the requirements of Action Plan 1 in the 2011 Oak Ridge Reservation-wide Comprehensive Environmental Response, Compensation, and Liability Act Five Year Review (FYR). The Action Plan is designed to address a twofold 2011 FYR issue: (1) new information suggests mobilization of mercury from the upper and lower EFPC streambeds and stream banks is the primary source of mercury export during high-flow conditions, and (2) the current Record of Decision did not address the entire hydrologic system and creek bank or creek bed sediments. To obtain a more robust watershed-scale understanding of mercury sources and processes in lower EFPC (LEFPC), new field and laboratory studies were coupled with existing data from multiple US Department of Energy programs to develop a dynamic watershed and bioaccumulation model. LEFPC field studies for the project focused primarily on quantification of streambank erosion and an evaluation of mercury dynamics in shallow groundwater adjacent to LEFPC and potential connection to the surface water. The approach to the stream bank study was innovative in using imagery from kayak floats' surveys from the headwaters to the mouth of EFPC to estimate erosion, coupled with detailed bank soil mercury analyses. The goal of new field assessments and modeling was to generate a more holistic and quantitative understanding of the watershed and the sources, flux, concentration, transformation, and bioaccumulation of inorganic mercury (IHg) and methylmercury (MeHg). Model development used a hybrid approach that dynamically linked a spreadsheet-based physical and chemical watershed model to a systems dynamics, mercury bioaccumulation model for key fish species. The watershed model tracks total Hg and MeHg fluxes and concentrations by examining upstream inputs, floodplain

  14. Evaluation of Lower East Fork Poplar Creek Mercury Sources

    Energy Technology Data Exchange (ETDEWEB)

    Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bevelhimer, Mark S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Chris [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brandt, Craig C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ketelle, Richard [East Tennessee Technology Park (ETTP), Oak Ridge, TN (United States)

    2016-06-01

    This report summarizes a 3-year research project undertaken to better understand the nature and magnitude of mercury (Hg) fluxes in East Fork Poplar Creek (EFPC). This project addresses the requirements of Action Plan 1 in the 2011 Oak Ridge Reservation-wide Comprehensive Environmental Response, Compensation, and Liability Act Five Year Review (FYR). The Action Plan is designed to address a twofold 2011 FYR issue: (1) new information suggests mobilization of mercury from the upper and lower EFPC streambeds and stream banks is the primary source of mercury export during high-flow conditions, and (2) the current Record of Decision did not address the entire hydrologic system and creek bank or creek bed sediments. To obtain a more robust watershed-scale understanding of mercury sources and processes in lower EFPC (LEFPC), new field and laboratory studies were coupled with existing data from multiple US Department of Energy programs to develop a dynamic watershed and bioaccumulation model. LEFPC field studies for the project focused primarily on quantification of streambank erosion and an evaluation of mercury dynamics in shallow groundwater adjacent to LEFPC and potential connection to the surface water. The approach to the stream bank study was innovative in using imagery from kayak floats’ surveys from the headwaters to the mouth of EFPC to estimate erosion, coupled with detailed bank soil mercury analyses. The goal of new field assessments and modeling was to generate a more holistic and quantitative understanding of the watershed and the sources, flux, concentration, transformation, and bioaccumulation of inorganic mercury (IHg) and methylmercury (MeHg). Model development used a hybrid approach that dynamically linked a spreadsheet-based physical and chemical watershed model to a systems dynamics, mercury bioaccumulation model for key fish species. The watershed model tracks total Hg and MeHg fluxes and concentrations by examining upstream inputs, floodplain

  15. Environmental Assessment - Construct a Ground-to-Air Transmitter and Receiver (GATR) Facility at Grand Forks Air Force Base

    National Research Council Canada - National Science Library

    2006-01-01

    ...) facility on Grand Forks Air Force Base (AFB), North Dakota. The Communication Squadron is preparing to install new GATR communication antennas and systems, for tactical aircraft control and commercial air traffic control...

  16. Turbo Charge CPU Utilization in Fork/Join Using the ManagedBlocker

    CERN Multimedia

    CERN. Geneva

    2017-01-01

    Fork/Join is a framework for parallelizing calculations using recursive decomposition, also called divide and conquer. These algorithms occasionally end up duplicating work, especially at the beginning of the run. We can reduce wasted CPU cycles by implementing a reserved caching scheme. Before a task starts its calculation, it tries to reserve an entry in the shared map. If it is successful, it immediately begins. If not, it blocks until the other thread has finished its calculation. Unfortunately this might result in a significant number of blocked threads, decreasing CPU utilization. In this talk we will demonstrate this issue and offer a solution in the form of the ManagedBlocker. Combined with the Fork/Join, it can keep parallelism at the desired level.

  17. Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork

    Energy Technology Data Exchange (ETDEWEB)

    Ben-Hur, E [Israel Atomic Energy Commission, Beersheba. Nuclear Research Center-Negev; Hagan, M P [Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)

    1984-05-01

    Chinese hamster fibroblasts were pulse labelled with 5-bromodeoxyuridine and exposed at time intervals (Tsub(i)) to near-ultraviolet (U.V.A.) light in the presence of a bisbenzimidazole derivative (Hoechst 33342). The sensitivity of the cells in terms of colony forming ability fluctuated depending on Tsub(i). Inhibition of DNA synthesis also depended on Tsub(i) and was maximal when Tsub(i)=O. Using the alkaline elution technique it was shown that the effect of a large dose of light was to inhibit both initiation and elongation of DNA chains. These effects were most pronounced for Tsub(i)=O. It is concluded that DNA damage in an active replicon can inhibit initiation of new replicons and that damage localized behind the replication fork can retard elongation of nascent DNA chains. This effect on chain elongation decreases with increased distance of the damage from the replication fork.

  18. Recombination at DNA replication fork barriers is not universal and is differentially regulated by Swi1.

    Science.gov (United States)

    Pryce, David W; Ramayah, Soshila; Jaendling, Alessa; McFarlane, Ramsay J

    2009-03-24

    DNA replication stress has been implicated in the etiology of genetic diseases, including cancers. It has been proposed that genomic sites that inhibit or slow DNA replication fork progression possess recombination hotspot activity and can form potential fragile sites. Here we used the fission yeast, Schizosaccharomyces pombe, to demonstrate that hotspot activity is not a universal feature of replication fork barriers (RFBs), and we propose that most sites within the genome that form RFBs do not have recombination hotspot activity under nonstressed conditions. We further demonstrate that Swi1, the TIMELESS homologue, differentially controls the recombination potential of RFBs, switching between being a suppressor and an activator of recombination in a site-specific fashion.

  19. Mercury Content of Sediments in East Fork Poplar Creek: Current Assessment and Past Trends

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Eller, Virginia A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dickson, Johnbull O. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Earles, Jennifer E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lowe, Kenneth Alan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mehlhorn, Tonia L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Olsen, Todd A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Watson, David J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Phillips, Debra H. [Queen' s Univ., Belfast (United Kingdom); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2017-01-01

    This study provided new information on sediment mercury (Hg) and monomethylmercury (MMHg) content and chemistry. The current inventory of Hg in East Fork Poplar Creek (EFPC) bed sediments was estimated to be 334 kg, which represents a ~67% decrease relative to the initial investigations in 1984. MMHg sediment inventory was estimated to be 44.1 g, lower but roughly similar to past estimates. The results support the relevance and potential impacts of other active and planned investigations within the Mercury Remediation Technology Development for Lower East Fork Poplar Creek project (e.g., assessment and control of bank soil inputs, sorbents for Hg and MMHg removal, re-introduction of freshwater clams to EFPC), and identify gaps in current understanding that represent opportunities to understand controlling variables that may inform future technology development studies.

  20. Groundwater and surface-water interaction within the upper Smith River Watershed, Montana 2006-2010

    Science.gov (United States)

    Caldwell, Rodney R.; Eddy-Miller, Cheryl A.

    2013-01-01

    (increased groundwater levels relative to stream stage) or even reversed gradient direction at several monitoring sites coincident with the onset of nearby flood irrigation. Groundwater-level declines in mid-summer were due to groundwater withdrawals and reduced recharge from decreased precipitation, increased evapotranspiration, and reduced leakage in some area streams during periods of low flow. Groundwater levels typically rebounded in late summer, a result of decreased evapotranspiration, decreased groundwater use for irrigation, increased flow in losing streams, and the onset of late-season flood irrigation at some sites. The effect of groundwater and surface-water interactions is most apparent along the North and South Forks of the Smith River where the magnitude of streamflow losses and gains can be greater than the magnitude of flow within the stream. Net gains consistently occurred over the lower 15 miles of the South Fork Smith River. A monitoring site near the mouth of the South Fork Smith River gained (flow from the groundwater to the stream) during all seasons, with head gradients towards the stream. Two upstream sites on the South Fork Smith River exhibited variable conditions that ranged from gaining during the spring, losing (flowing from the stream to the groundwater) during most of the summer as groundwater levels declined, and then approached or returned to gaining conditions in late summer. Parts of the South Fork Smith River became dry during periods of losing conditions, thus classifying this tributary as intermittent. The North Fork Smith River is highly managed at times through reservoir releases. The North Fork Smith River was perennial throughout the study period although irrigation diversions removed a large percentage of streamflow at times and losing conditions persisted along a lower reach. The lowermost reach of the North Fork Smith River near its mouth transitioned from a losing reach to a gaining reach throughout the study period. Groundwater

  1. Temperature, Frequency, and Young’s Modulus of an Aluminum Tuning Fork

    Directory of Open Access Journals (Sweden)

    Zachery L. Greer

    2011-01-01

    Full Text Available The frequency produced by a standard C (523.3 Hz aluminum alloy tuning fork when struck at temperatures ranging from 29 ̊C to 300 ̊C was studied. It was found that frequency decreased with increasing temperature with an inverse exponential relationship. The frequency was used to calculate Young’s Modulus for aluminum, with the results being in close agreement with published values.

  2. Gaussian Random Fields Methods for Fork-Join Network with Synchronization Constraints

    Science.gov (United States)

    2014-12-22

    substantial efforts were dedicated to the study of the max-plus recursions [21, 3, 12]. More recently, Atar et al. [2] have studied a fork-join...feedback and NES, Atar et al. [2] show that a dynamic priority discipline achieves throughput optimal- ity asymptotically in the conventional heavy...2011) Patient flow in hospitals: a data-based queueing-science perspective. Submitted to Stochastic Systems, 20. [2] R. Atar , A. Mandelbaum and A

  3. Human Costs Assessment - The Impacts of Flooding & Nonstructural Solutions. Tug Fork Valley, West Virginia & Kentucky.

    Science.gov (United States)

    1980-04-01

    to the outside world (especially in the West Virginia side). New people are being drawn in and there is a continuing out migration. Franchised ...all of these businesses are local ones suggesting the skillful entrepreneurship of area people and the recognition of Goody’s existence in the larger...chain stores and franchise businesses. The net effect is that here is another instance in which the Tug Fork people are dependent upon outsiders. King

  4. Escherichia coli DinB inhibits replication fork progression without significantly inducing the SOS response.

    Science.gov (United States)

    Mori, Tetsuya; Nakamura, Tatsuro; Okazaki, Naoto; Furukohri, Asako; Maki, Hisaji; Akiyama, Masahiro Tatsumi

    2012-01-01

    The SOS response is readily triggered by replication fork stalling caused by DNA damage or a dysfunctional replicative apparatus in Escherichia coli cells. E. coli dinB encodes DinB DNA polymerase and its expression is upregulated during the SOS response. DinB catalyzes translesion DNA synthesis in place of a replicative DNA polymerase III that is stalled at a DNA lesion. We showed previously that DNA replication was suppressed without exogenous DNA damage in cells overproducing DinB. In this report, we confirm that this was due to a dose-dependent inhibition of ongoing replication forks by DinB. Interestingly, the DinB-overproducing cells did not significantly induce the SOS response even though DNA replication was perturbed. RecA protein is activated by forming a nucleoprotein filament with single-stranded DNA, which leads to the onset of the SOS response. In the DinB-overproducing cells, RecA was not activated to induce the SOS response. However, the SOS response was observed after heat-inducible activation in strain recA441 (encoding a temperature-sensitive RecA) and after replication blockage in strain dnaE486 (encoding a temperature-sensitive catalytic subunit of the replicative DNA polymerase III) at a non-permissive temperature when DinB was overproduced in these cells. Furthermore, since catalytically inactive DinB could avoid the SOS response to a DinB-promoted fork block, it is unlikely that overproduced DinB takes control of primer extension and thus limits single-stranded DNA. These observations suggest that DinB possesses a feature that suppresses DNA replication but does not abolish the cell's capacity to induce the SOS response. We conclude that DinB impedes replication fork progression in a way that does not activate RecA, in contrast to obstructive DNA lesions and dysfunctional replication machinery.

  5. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress

    Science.gov (United States)

    Magdalou, Indiana; Machon, Christelle; Dardillac, Elodie; Técher, Hervé; Guitton, Jérôme; Debatisse, Michelle; Lopez, Bernard S.

    2016-01-01

    Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es). Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3%) rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing), and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and senescence initiation

  6. Uncertainty Quantification of Fork Detector Measurements from Spent Fuel Loading Campaigns

    International Nuclear Information System (INIS)

    Vaccaro, S.; De Baere, P.; Schwalbach, P.; Gauld, I.; Hu, J.

    2015-01-01

    With increasing activities at the end of the fuel cycle, the requirements for the verification of spent nuclear fuel for safeguards purposes are continuously growing. In the European Union we are experiencing a dramatic increase in the number of cask loadings for interim dry storage. This is caused by the progressive shut-down of reactors, related to facility ageing but also due to politically motivated phase-out of nuclear power. On the other hand there are advanced plans for the construction of encapsulation plants and geological repositories. The cask loading or the encapsulation process will provide the last occasion to verify the spent fuel assemblies. In this context, Euratom and the US DOE have carried out a critical review of the widely used Fork measurements method of irradiated assemblies. The Nuclear Safeguards directorates of the European Commission's Directorate General for Energy and Oak Ridge National Laboratory have collaborated to improve the Fork data evaluation process and simplify its use for inspection applications. Within the Commission's standard data evaluation package CRISP, we included a SCALE/ORIGEN-based irradiation and depletion simulation of the measured assembly and modelled the fork transfer function to calculate expected count rates based on operator's declarations. The complete acquisition and evaluation process has been automated to compare expected (calculated) with measured count rates. This approach allows a physics-based improvement of the data review and evaluation process. At the same time the new method provides the means for better measurement uncertainty quantification. The present paper will address the implications of the combined approach involving measured and simulated data to the quantification of measurement uncertainty and the consequences of these uncertainties in the possible use of the Fork detector as a partial defect detection method. (author)

  7. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress.

    Directory of Open Access Journals (Sweden)

    Therese Wilhelm

    2016-05-01

    Full Text Available Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es. Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3% rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing, and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and

  8. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress.

    Science.gov (United States)

    Wilhelm, Therese; Ragu, Sandrine; Magdalou, Indiana; Machon, Christelle; Dardillac, Elodie; Técher, Hervé; Guitton, Jérôme; Debatisse, Michelle; Lopez, Bernard S

    2016-05-01

    Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es). Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3%) rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing), and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and senescence initiation

  9. NDA measurements on spent fuel assemblies at Tihange 1 by means of the ION 1/FORK

    International Nuclear Information System (INIS)

    Carchon, R.; Smaers, G.; Verrecchia, G.P.D.; Arlt, R.; Stoyanova, I.; Satinet, J.

    1986-06-01

    This report describes field tests performed at Tihange 1 Nuclear Power Station on PWR spent fuel by means of the ION 1-FORK detector. Two detector systems and three electronics systems were used to investigate the same fuel assemblies with various burn-ups and cooling times. The purpose of the exercise was to test the performance of the instrument for as well inspection purposes as for fuel management. The results are presented and discussed. (Author)

  10. Charles River

    Science.gov (United States)

    Information on the efforts of the US EPA, the Commonwealth of Massachusetts, the municipalities within the Charles River Watershed and nongovernmental organizations to improve the water quality of the Charles River.

  11. Burnup verification measurements at a US nuclear utility using the FORK measurement system

    International Nuclear Information System (INIS)

    Ewing, R.I.; Bosler, G.E.; Walden, G.

    1993-01-01

    The FORK measurement system, designed at Los Alamos National Laboratory (LANL) for the International Atomic Energy Agency (IAEA) safeguards program, has been used to examine spent reactor fuel assemblies at Duke Power Company's Oconee Nuclear Station. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. These measurements can be correlated with burnup and cooling time, and can be used to verify the reactor site records. Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. By taking into account the reduced reactivity of spent fuel due to its burnup in the reactor, burnup credit results in more efficient and economic transport and storage. The objectives of these tests are to demonstrate the applicability of the FORK system to verify reactor records and to develop optimal procedures compatible with utility operations. The test program is a cooperative effort supported by Sandia National Laboratories, the Electric Power Research Institute (EPRI), Los Alamos National Laboratory, and the Duke Power Company

  12. MOF Suppresses Replication Stress and Contributes to Resolution of Stalled Replication Forks.

    Science.gov (United States)

    Singh, Dharmendra Kumar; Pandita, Raj K; Singh, Mayank; Chakraborty, Sharmistha; Hambarde, Shashank; Ramnarain, Deepti; Charaka, Vijaya; Ahmed, Kazi Mokim; Hunt, Clayton R; Pandita, Tej K

    2018-03-15

    The human MOF (hMOF) protein belongs to the MYST family of histone acetyltransferases and plays a critical role in transcription and the DNA damage response. MOF is essential for cell proliferation; however, its role during replication and replicative stress is unknown. Here we demonstrate that cells depleted of MOF and under replicative stress induced by cisplatin, hydroxyurea, or camptothecin have reduced survival, a higher frequency of S-phase-specific chromosome damage, and increased R-loop formation. MOF depletion decreased replication fork speed and, when combined with replicative stress, also increased stalled replication forks as well as new origin firing. MOF interacted with PCNA, a key coordinator of replication and repair machinery at replication forks, and affected its ubiquitination and recruitment to the DNA damage site. Depletion of MOF, therefore, compromised the DNA damage repair response as evidenced by decreased Mre11, RPA70, Rad51, and PCNA focus formation, reduced DNA end resection, and decreased CHK1 phosphorylation in cells after exposure to hydroxyurea or cisplatin. These results support the argument that MOF plays an important role in suppressing replication stress induced by genotoxic agents at several stages during the DNA damage response. Copyright © 2018 American Society for Microbiology.

  13. Far-travelled permian chert of the North Fork terrane, Klamath mountains, California

    Science.gov (United States)

    Mankinen, E.A.; Irwin, W.P.; Blome, C.D.

    1996-01-01

    Permian chert in the North Fork terrane and correlative rocks of the Klamath Mountains province has a remanent magnetization that is prefolding and presumably primary. Paleomagnetic results indicate that the chert formed at a paleolatitude of 8.6?? ?? 2.5?? but in which hemisphere remains uncertain. This finding requires that these rocks have undergone at least 8.6?? ?? 4.4?? of northward transport relative to Permian North America since their deposition. Paleontological evidence suggests that the Permian limestone of the Eastern Klamath terrane originated thousands of kilometers distant from North America. The limestone of the North Fork terrane may have formed at a similar or even greater distance as suggested by its faunal affinity to the Eastern Klamath terrane and more westerly position. Available evidence indicates that convergence of the North Fork and composite Central Metamorphic-Eastern Klamath terranes occurred during Triassic or Early Jurassic time and that their joining together was a Middle Jurassic event. Primary and secondary magnetizations indicate that the new composite terrane containing these and other rocks of the Western Paleozoic and Triassic belt behaved as a single rigid block that has been latitudinally concordant with the North American craton since Middle Jurassic time.

  14. The progression of replication forks at natural replication barriers in live bacteria.

    Science.gov (United States)

    Moolman, M Charl; Tiruvadi Krishnan, Sriram; Kerssemakers, Jacob W J; de Leeuw, Roy; Lorent, Vincent; Sherratt, David J; Dekker, Nynke H

    2016-07-27

    Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in the cell are poorly understood. By performing quantitative fluorescence microscopy with microfuidics, we investigate the effect on the replisome when encountering these barriers in live Escherichia coli cells. We make use of an E. coli variant that includes only an ectopic origin of replication that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other replisome. This enables us to single out the effect of encountering a Tus-ter roadblock on an individual replisome. We demonstrate that the replisome remains stably bound after encountering a Tus-ter complex from the non-permissive direction. Furthermore, the replisome is only transiently blocked, and continues replication beyond the barrier. Additionally, we demonstrate that these barriers affect sister chromosome segregation by visualizing specific chromosomal loci in the presence and absence of the Tus protein. These observations demonstrate the resilience of the replication fork to natural barriers and the sensitivity of chromosome alignment to fork progression. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  15. Kinbasket Reservoir and Upper Columbia River Kokanee spawner index 2005

    International Nuclear Information System (INIS)

    Manson, H.; Porto, L.

    2006-01-01

    The results of an escapement survey for tributaries to the Kinbasket Reservoir and the Upper Columbia River were provided. Two aerial surveys were conducted during October, 2005. The Kokanee were grouped in schools and summed in order to provide independent estimates. Otoliths of the fish were also extracted in order to determine their age. Results of the survey showed that an estimated 236,760 Kokanee fish were spawning within 11 index streams and rivers within the Kinbasket Reservoir drainage area. Mean fork length was estimated at 24.7 cm. While the Columbia River continues to be the most important Kokanee spawning location in the Kinbasket Reservoir drainage area, the 2005 Kokanee escapement index was the third lowest recorded since 1996. It was concluded that declining fish size and declining abundance may indicate reduced reservoir productivity. 5 refs., 1 tab., 4 figs

  16. The effectiveness of front fork systems at damping accelerations during isolated aspects specific to cross-country mountain biking.

    Science.gov (United States)

    Macdermid, Paul W; Miller, Matthew C; Fink, Philip W; Stannard, Stephen R

    2017-11-01

    Cross-country mountain bike suspension reportedly enhances comfort and performance through reduced vibration and impact exposure. This study analysed the effectiveness of three different front fork systems at damping accelerations during the crossing of three isolated obstacles (stairs, drop, and root). One participant completed three trials on six separate occasions in a randomised order using rigid, air-sprung, and carbon leaf-sprung forks. Performance was determined by time to cross obstacles, while triaxial accelerometers quantified impact exposure and damping response. Results identified significant main effect of fork type for performance time (p < 0.05). The air-sprung and leaf-sprung forks were significantly slower than the rigid forks for the stairs (p < 0.05), while air-sprung suspension was slower than the rigid for the root protocol (p < 0.05). There were no differences for the drop protocol (p < 0.05). Rigid forks reduced overall exposure (p < 0.05), specifically at the handlebars for the stairs and drop trials. More detailed analysis presented smaller vertical accelerations at the handlebar for air-sprung and leaf-sprung forks on the stairs (p < 0.05), and drop (p < 0.05) but not the root. As such, it appears that the suspension systems tested were ineffective at reducing overall impact exposure at the handlebar during isolated aspects of cross-country terrain features which may be influenced to a larger extent by rider technique.

  17. Assessment of conservation easements, total phosphorus, and total suspended solids in West Fork Beaver Creek, Minnesota, 1999-2012

    Science.gov (United States)

    Christensen, Victoria G.; Kieta, Kristen A.

    2014-01-01

    This study examined conservation easements and their effectiveness at reducing phosphorus and solids transport to streams. The U.S. Geological Survey cooperated with the Minnesota Board of Water and Soil Resources and worked collaboratively with the Hawk Creek Watershed Project to examine the West Fork Beaver Creek Basin in Renville County, which has the largest number of Reinvest In Minnesota land retirement contracts in the State (as of 2013). Among all conservation easement programs, a total of 24,218 acres of agricultural land were retired throughout Renville County, and 2,718 acres were retired in the West Fork Beaver Creek Basin from 1987 through 2012. Total land retirement increased steadily from 1987 until 2000. In 2000, land retirement increased sharply because of the Minnesota River Conservation Reserve Enhancement Program, then leveled off when the program ended in 2002. Streamflow data were collected during 1999 through 2011, and total phosphorus and total suspended solids data were collected during 1999 through 2012. During this period, the highest peak streamflow of 1,320 cubic feet per second was in March 2010. Total phosphorus and total suspended solids are constituents that tend to increase with increases in streamflow. Annual flow-weighted mean total-phosphorus concentrations ranged from 0.140 to 0.759 milligrams per liter, and annual flow-weighted mean total suspended solids concentrations ranged from 21.3 to 217 milligrams per liter. Annual flow-weighted mean total phosphorus and total suspended solids concentrations decreased steadily during the first 4 years of water-quality sample collection. A downward trend in flow-weighted mean total-phosphorus concentrations was significant from 1999 through 2008; however, flow-weighted total-phosphorus concentrations increased substantially in 2009, and the total phosphorus trend was no longer significant. The high annual flow-weighted mean concentrations for total phosphorus and total suspended solids

  18. Selected trace-element and organic contaminants in the streambed sediments of the Potomac River Basin, August 1992

    Science.gov (United States)

    Gerhart, James M.; Blomquist, Joel D.

    1995-01-01

    This report describes the occurrence and distribution of five selected contaminants in streambed sediments at 22 stream sites in the Potomac River Basin. Lead, mercury, and total DDT (dichlorodiphenyltrichloroethane) were detected at all sites, and chlordane and total PCB's (polychlorinated biphenyls) were detected at most sites. At six sites, streambed-sediment concentrations of contaminants were detected at levels with the potential to cause frequent adverse effects on aquatic organisms that live in the sediments. Chlordane was detected at these high levels at sampling sites on the Anacostia River, the North Branch Potomac River, Bull Run, and Accotink Creek; mercury was detected at these levels at sites on the South River and the South Fork Shenandoah River; and total PCB's were detected at these levels at the site on the South Fork Shenandoah River. The highest concentrations of all five contaminants generally occurred at sampling sites downstream from areas with industrial plants, urban centers, or orchard and agricultural activity. The occurrence of these contaminants in streambed sediments of the Potomac River Basin is of concern because the contaminants (1) are environmentally persistent, (2) are available for downstream transport during high streamflow periods, and (3) have the potential to cause adverse effects on the health of aquatic organisms and humans through bioaccumulation.

  19. Use of preserved museum fish to evaluate historical and current mercury contamination in fish from two rivers in Oklahoma, USA.

    Science.gov (United States)

    Hill, J Jaron; Chumchal, Matthew M; Drenner, Ray W; Pinder, John E; Drenner, S Matthew

    2010-02-01

    We examined the effects of a commonly used preservation technique on mercury concentration in fish tissue. After fixing fish muscle tissue in formalin followed by preservation in isopropanol, we found that mercury concentration in fish muscle tissue increased by 18%, reaching an asymptote after 40 days. We used formalin-isopropanol-preserved longear sunfish (Lepomis megalotis) from the Sam Noble Oklahoma Museum of Natural History to examine historical changes and predict current mercury concentrations in fish from two rivers in southeastern Oklahoma. Glover River was free-flowing, while Mountain Fork River was impounded in 1970 and a coldwater trout fishery was established upstream from the collection site in 1989. Mercury concentrations in longear sunfish from Glover River showed no historical changes from 1963 to 2001. Mercury concentrations in longear sunfish from Mountain Fork River showed no change from 1925 to 1993 but declined significantly from 1993 to 2003. We also compared mercury concentrations of the most recently collected longear sunfish in the museum to mercury concentrations of unpreserved fish collected from the rivers in 2006. Concentrations of mercury in museum fish were not significantly different from mercury concentrations in unpreserved fish we collected from the rivers. Our study indicates that preserved museum fish specimens can be used to evaluate historical changes and predict current levels of mercury contamination in fish.

  20. Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.

    Science.gov (United States)

    Parajuli, Shankar; Teasley, Daniel C; Murali, Bhavna; Jackson, Jessica; Vindigni, Alessandro; Stewart, Sheila A

    2017-09-15

    Faithful DNA replication is essential for genome stability. To ensure accurate replication, numerous complex and redundant replication and repair mechanisms function in tandem with the core replication proteins to ensure DNA replication continues even when replication challenges are present that could impede progression of the replication fork. A unique topological challenge to the replication machinery is posed by RNA-DNA hybrids, commonly referred to as R-loops. Although R-loops play important roles in gene expression and recombination at immunoglobulin sites, their persistence is thought to interfere with DNA replication by slowing or impeding replication fork progression. Therefore, it is of interest to identify DNA-associated enzymes that help resolve replication-impeding R-loops. Here, using DNA fiber analysis, we demonstrate that human ribonuclease H1 (RNH1) plays an important role in replication fork movement in the mammalian nucleus by resolving R-loops. We found that RNH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage. Our data uncovered a role for RNH1 in global DNA replication in the mammalian nucleus. Because accumulation of RNA-DNA hybrids is linked to various human cancers and neurodegenerative disorders, our study raises the possibility that replication fork progression might be impeded, adding to increased genomic instability and contributing to disease. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  1. Dynamic behavior of tuning fork shear-force structures in a SNOM system

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Fengli [Department of Engineering Mechanics, AML, CNMM, Tsinghua University, Beijing 100084 (China); Li, Xide, E-mail: lixide@tsinghua.edu.cn [Department of Engineering Mechanics, AML, CNMM, Tsinghua University, Beijing 100084 (China); Wang, Jia [State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing 100084 (China); Fu, Yu [Temasek Laboratories, Nanyang Technological University, 50 Nanyang Drive, 637553 (Singapore)

    2014-07-01

    Piezoelectric tuning fork shear-force structures are widely used as a distance control unit in a scanning near-field optical microscopy. However, the complex dynamic behavior among the micro-tuning forks (TFs), optical fiber probes, and the probe–surface interactions is still a crucial issue to achieve high-resolution imaging or near-field interaction inspections. Based on nonlinear beam tension-bending vibration theory, vibration equations in both longitudinal and lateral directions have been established when the TF structure and the optical fiber are treated as deformable structures. The relationship of the probe–surface interaction induced by Van der Waals force has been analyzed and the corresponding numerical results used to describe the vibrational behavior of the probe approaching the sample surface are obtained. Meanwhile, the viscous resistance of the liquid film on the sample surface has also been investigated using linear beam-bending vibration theory. Experiments testing the interaction between the probe and the water film on a single crystal silicon wafer have been carried out and the viscous resistance of the water film was estimated using the established equations. Finally, to use the TF-probe structure as a force sensor, the relation between the dynamic response of the TF-probe system and an external force on the probe tip was obtained. - Highlights: • Nonlinear vibration equation is established for a deformable tuning fork probe assembly. • Probe–sample interactions induced by Van der Waals force and viscous resistance are investigated. • The viscous resistance between the probe and the water film is estimated using testing results.

  2. In-Field Performance Testing of the Fork Detector for Quantitative Spent Fuel Verification

    International Nuclear Information System (INIS)

    Gauld, Ian C.; Hu, Jianwei; De Baere, P.; Tobin, Stephen

    2015-01-01

    Expanding spent fuel dry storage activities worldwide are increasing demands on safeguards authorities that perform inspections. The European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) require measurements to verify declarations when spent fuel is transferred to difficult-to-access locations, such as dry storage casks and the repositories planned in Finland and Sweden. EURATOM makes routine use of the Fork detector to obtain gross gamma and total neutron measurements during spent fuel inspections. Data analysis is performed by modules in the integrated Review and Analysis Program (iRAP) software, developed jointly by EURATOM and the IAEA. Under the framework of the US Department of Energy-EURATOM cooperation agreement, a module for automated Fork detector data analysis has been developed by Oak Ridge National Laboratory (ORNL) using the ORIGEN code from the SCALE code system and implemented in iRAP. EURATOM and ORNL recently performed measurements on 30 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel (Clab), operated by the Swedish Nuclear Fuel and Waste Management Company (SKB). The measured assemblies represent a broad range of fuel characteristics. Neutron count rates for 15 measured pressurized water reactor assemblies are predicted with an average relative standard deviation of 4.6%, and gamma signals are predicted on average within 2.6% of the measurement. The 15 measured boiling water reactor assemblies exhibit slightly larger deviations of 5.2% for the gamma signals and 5.7% for the neutron count rates, compared to measurements. These findings suggest that with improved analysis of the measurement data, existing instruments can provide increased verification of operator declarations of the spent fuel and thereby also provide greater ability to confirm integrity of an assembly. These results support the application of the Fork detector as a fully quantitative spent fuel

  3. In-Field Performance Testing of the Fork Detector for Quantitative Spent Fuel Verification

    Energy Technology Data Exchange (ETDEWEB)

    Gauld, Ian C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Hu, Jianwei [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); De Baere, P. [European Commission (Luxembourg). DG Energy, Directorate Nuclear Safeguards; Vaccaro, S. [European Commission (Luxembourg). DG Energy, Directorate Nuclear Safeguards; Schwalbach, P. [European Commission (Luxembourg). DG Energy, Directorate Nuclear Safeguards; Liljenfeldt, Henrik [Swedish Nuclear Fuel and Waste Management Company (Sweden); Tobin, Stephen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-01-01

    Expanding spent fuel dry storage activities worldwide are increasing demands on safeguards authorities that perform inspections. The European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) require measurements to verify declarations when spent fuel is transferred to difficult-to-access locations, such as dry storage casks and the repositories planned in Finland and Sweden. EURATOM makes routine use of the Fork detector to obtain gross gamma and total neutron measurements during spent fuel inspections. Data analysis is performed by modules in the integrated Review and Analysis Program (iRAP) software, developed jointly by EURATOM and the IAEA. Under the framework of the US Department of Energy–EURATOM cooperation agreement, a module for automated Fork detector data analysis has been developed by Oak Ridge National Laboratory (ORNL) using the ORIGEN code from the SCALE code system and implemented in iRAP. EURATOM and ORNL recently performed measurements on 30 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel (Clab), operated by the Swedish Nuclear Fuel and Waste Management Company (SKB). The measured assemblies represent a broad range of fuel characteristics. Neutron count rates for 15 measured pressurized water reactor assemblies are predicted with an average relative standard deviation of 4.6%, and gamma signals are predicted on average within 2.6% of the measurement. The 15 measured boiling water reactor assemblies exhibit slightly larger deviations of 5.2% for the gamma signals and 5.7% for the neutron count rates, compared to measurements. These findings suggest that with improved analysis of the measurement data, existing instruments can provide increased verification of operator declarations of the spent fuel and thereby also provide greater ability to confirm integrity of an assembly. These results support the application of the Fork detector as a fully quantitative spent fuel

  4. Summary of biological investigations relating to surface-water quality in the Kentucky River Basin, Kentucky

    International Nuclear Information System (INIS)

    Bradfield, A.D.; Porter, S.D.

    1990-01-01

    The Kentucky River basin, an area of approximately 7,000 sq mi, is divided into five hydrologic units that drain parts of three physiographic regions. Data on aquatic biological resources were collected and reviewed to assess conditions in the major streams for which data were available. The North, Middle, and south Forks of the Kentucky River are in the Eastern Coal Field physiographic region. Streams in this region are affected by drainage from coal mines and oil and gas operations, and many support only tolerant biotic stream forms. The Kentucky River from the confluence of the three forks to the Red River, is in the Knobs physiographic region. Oil and gas production operations and point discharges from municipalities have affected many streams in this region. The Red River, a Kentucky Wild River, supported a unique flora and fauna but accelerated sedimentation has eliminated many species of mussels. The Millers Creek drainage is affected by brines discharged from oil and gas operations, and some reaches support only halophilic algae and a few fish. The Kentucky River from the Red River to the Ohio River is in the Bluegrass physiographic region. Heavy sediment loads and sewage effluent from urban centers have limited the aquatic biota in this region. Silver Creek and South Elkhorn Creek have been particularly affected and aquatic communities in these streams are dominated by organisms tolerant of low dissolved oxygen concentrations. Biological data for other streams indicate that habitat and water quality conditions are favorable for most commonly occurring aquatic organisms. 205 refs., 7 figs., 1 tab

  5. Performance Characteristics and Temperature Compensation Method of Fluid Property Sensor Based on Tuning-Fork Technology

    Directory of Open Access Journals (Sweden)

    Yuan Chen

    2016-01-01

    Full Text Available Fluid property sensor (FPS based on tuning-fork technology is applied to the measurement of the contaminant level of lubricant oil. The measuring principle of FPS sensor is derived and proved together with its resolution. The performance characteristics of the FPS sensor, such as sensitivity coefficient, resolution, and quality factor, are analyzed. A temperature compensation method is proposed to eliminate the temperature-dependence of the measuring parameters, and its validity is investigated by numerical simulation of sensitivity, oscillating frequency, and dielectric constant. The values of purification efficiency obtained using microwave and without microwave are compared experimentally.

  6. Evaluation of Lower East Fork Poplar Creek Mercury Sources - Model Update

    Energy Technology Data Exchange (ETDEWEB)

    Ketelle, Richard [East Tennessee Technology Park (ETTP), Oak Ridge, TN (United States); Brandt, Craig C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bevelhimer, Mark S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mayes, Melanie [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dickson, Johnbull O. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Olsen, Todd A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2017-08-01

    The purpose of this report is to assess new data that has become available and provide an update to the evaluations and modeling presented in the Oak Ridge National Laboratory (ORNL) Technical Manuscript Evaluation of lower East Fork Poplar Creek (LEFPC) Mercury Sources (Watson et al., 2016). Primary sources of field and laboratory data for this update include multiple US Department of Energy (DOE) programs including Environmental Management (EM; e.g., Biological Monitoring and Abatement Program, Mercury Remediation Technology Development [TD], and Applied Field Research Initiative), Office of Science (Mercury Science Focus Areas [SFA] project), and the Y-12 National Security Complex (Y-12) Compliance Department.

  7. Best management practices plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This plan was prepared in support of the Phase II Remedial Design Report (DOE/OR/01-1449 ampersand D1) and in accordance with requirements under CERCLA to present the plan for best management practices to be followed during the remediation. This document provides the Environmental Restoration Program with information about spill prevention and control, water quality monitoring, good housekeeping practices, sediment and erosion control measures, and inspections and environmental compliance practices to be used during Phase II of the remediation project for the Lower East Fork Poplar Creek Operable Unit

  8. Mercury Remediation Technology Development for Lower East Fork Poplar Creek - FY 2016 Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Dickson, Johnbull O. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Smith, John G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Mehlhorn, Tonia L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Lowe, Kenneth Alan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Morris, Jesse G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Mayes, Melanie [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Johs, Alexander [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); McManamay, Ryan A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Poteat, Monica D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Olsen, Todd A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Eller, Virginia A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Gonez Rodriguez, Leroy [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

    2017-07-01

    Mercury remediation is a high priority for the US Department of Energy (DOE) Oak Ridge Office of Environmental Management (OREM), especially at and near the Y-12 National Security Complex (Y-12) where historical mercury use has resulted in contaminated buildings, soils, and downstream surface waters. To address mercury contamination of East Fork Poplar Creek (EFPC), the DOE has adopted a phased, adaptive management approach to remediation, which includes mercury treatment actions at Y-12 in the short-term and research and technology development (TD) to evaluate longer-term solutions in the downstream environment (US Department of Energy 2014).

  9. Distance control for a near-field scanning microwave microscope in liquid using a quartz tuning fork

    International Nuclear Information System (INIS)

    Kim, Song Hul; Yoo, Hyun Jun; Yoo, Hyung Geun; Lee, Kie Jin

    2004-01-01

    We demonstrate a scanning near-field microwave microscope (NSMM) in the liquid environment using a tuning fork shear-force feedback method to control the distance between tip and sample. The probe tip for the NSMM is only immersed in water and attached to one prong of a quartz tuning fork and directly coupled to a high-quality dielectric resonator at an operating frequency f = 4.5-5.5 GHz. This distance control method is independent of the local microwave characteristics. The amplitude of the tuning fork was used as a set point of the distance control parameter in the liquid. To demonstrate the ability of the distance regulation system, we present the NSMM images of a copper film in air and liquid without and with readjusting the distance set point and a DNA film image in buffer solution.

  10. Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation.

    Science.gov (United States)

    Feng, Wenyi; Di Rienzi, Sara C; Raghuraman, M K; Brewer, Bonita J

    2011-10-01

    Chromosome breakage as a result of replication stress has been hypothesized to be the direct consequence of defective replication fork progression, or "collapsed" replication forks. However, direct and genome-wide evidence that collapsed replication forks give rise to chromosome breakage is still lacking. Previously we showed that a yeast replication checkpoint mutant mec1-1, after transient exposure to replication impediment imposed by hydroxyurea (HU), failed to complete DNA replication, accumulated single-stranded DNA (ssDNA) at the replication forks, and fragmented its chromosomes. In this study, by following replication fork progression genome-wide via ssDNA detection and by direct mapping of chromosome breakage after HU exposure, we have tested the hypothesis that the chromosome breakage in mec1 cells occurs at collapsed replication forks. We demonstrate that sites of chromosome breakage indeed correlate with replication fork locations. Moreover, ssDNA can be detected prior to chromosome breakage, suggesting that ssDNA accumulation is the common precursor to double strand breaks at collapsed replication forks.

  11. Salmonid Gamete Preservation in the Snake River Basin, Annual Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Young, William; Kucera, Paul

    2003-07-01

    In spite of an intensive management effort, chinook salmon (Oncorhynchus tshawytscha) and steelhead (Oncorhynchus mykiss) populations in the Northwest have not recovered and are currently listed as threatened species under the Endangered Species Act. In addition to the loss of diversity from stocks that have already gone extinct, decreased genetic diversity resulting from genetic drift and inbreeding is a major concern. Reduced population and genetic variability diminishes the environmental adaptability of individual species and entire ecological communities. The Nez Perce Tribe (NPT), in cooperation with Washington State University and the University of Idaho, established a germplasm repository in 1992 in order to preserve the remaining salmonid diversity in the region. The germplasm repository provides long-term storage for cryopreserved gametes. Although only male gametes can be cryopreserved, conserving the male component of genetic diversity will maintain future management options for species recovery. NPT efforts have focused on preserving salmon and steelhead gametes from the major river subbasins in the Snake River basin. However, the repository is available for all management agencies to contribute gamete samples from other regions and species. In 2002 a total of 570 viable semen samples were added to the germplasm repository. This included the gametes of 287 chinook salmon from the Lostine River, Catherine Creek, upper Grande Ronde River, Imnaha River (Lookingglass Hatchery), Lake Creek, South Fork Salmon River, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi River (Pahsimeroi Hatchery), and upper Salmon River (Sawtooth Hatchery) and the gametes of 280 steelhead from the North Fork Clearwater River (Dworshak Hatchery), Fish Creek, Little Sheep Creek, Pahsimeroi River (Pahsimeroi Hatchery) and Snake River (Oxbow Hatchery). In addition, gametes from 60 Yakima River spring chinook and 34 Wenatchee River coho salmon were added to the

  12. River nomads

    DEFF Research Database (Denmark)

    2016-01-01

    sail on the Niger River between Nigeria and Mali. Crossing villages, borders and cultures, they stop only to rest by setting up camp on riverbanks or host villages. In River Nomads, we join the nomadic Kebbawa fishermen on one of their yearly crossing, experiencing their relatively adventurous...

  13. River Piracy

    Indian Academy of Sciences (India)

    There was this highly venerated river Saraswati flowing through. Haryana, Marwar and Bahawalpur in Uttarapath and emptying itself in the Gulf ofKachchh, which has been described in glowing terms by the Rigveda. "Breaking through the mountain barrier", this "swift-flowing tempestuous river surpasses in majesty and.

  14. Atomic resolution ultrafast scanning tunneling microscope with scan rate breaking the resonant frequency of a quartz tuning fork resonator.

    Science.gov (United States)

    Li, Quanfeng; Lu, Qingyou

    2011-05-01

    We present an ultra-fast scanning tunneling microscope with atomic resolution at 26 kHz scan rate which surpasses the resonant frequency of the quartz tuning fork resonator used as the fast scan actuator. The main improvements employed in achieving this new record are (1) fully low voltage design (2) independent scan control and data acquisition, where the tuning fork (carrying a tip) is blindly driven to scan by a function generator with the scan voltage and tunneling current (I(T)) being measured as image data (this is unlike the traditional point-by-point move and measure method where data acquisition and scan control are switched many times).

  15. The Model Analysis of a Complex Tuning Fork Probe and Its Application in Bimodal Atomic Force Microscopy

    Directory of Open Access Journals (Sweden)

    Zhichao Wu

    2017-01-01

    Full Text Available A new electromechanical coupling model was built to quantitatively analyze the tuning fork probes, especially the complex ones. A special feature of a novel, soft tuning fork probe, that the second eigenfrequency of the probe was insensitive to the effective force gradient, was found and used in a homemade bimodal atomic force microscopy to measure power dissipation quantitatively. By transforming the mechanical parameters to the electrical parameters, a monotonous and concise method without using phase to calculate the power dissipation was proposed.

  16. Lentiform fork sign: a magnetic resonance finding in a case of acute metabolic acidosis.

    Science.gov (United States)

    Grasso, Daniela; Borreggine, Carmela; Perfetto, Francesco; Bertozzi, Vincenzo; Trivisano, Marina; Specchio, Luigi Maria; Grilli, Gianpaolo; Macarini, Luca

    2014-06-01

    We report a 33 year-old woman addicted to chronic unspecified solvents abuse with stupor, respiratory disorders, tetraplegia and severe metabolic acidosis. On admission an unenhanced cranial CT scan showed symmetrical hypodensities of both lentiform nuclei. MR imaging performed 12 hours after stupor demonstrates bilateral putaminal hemorrhagic necrosis, bilateral external capsule, corona radiata and deep cerebellar hyperintensities with right cingulate cortex involvement. DWI reflected bilateral putaminal hyperintensities with restricted water diffusion as to citotoxic edema and development of vasogenic edema in the external capsule recalling a fork. On day twenty, after specific treatments MRI demonstrated a bilateral putaminal marginal enhancement. Bilateral putaminal necrosis is a characteristic but non-specific radiological finding of methanol poisoning. Lentiform Fork sign is a rare MRI finding reported in literature in 22 patients with various conditions characterized by metabolic acidosis. Vasogenic edema may be due to the differences in metabolic vulnerability between neurons and astrocytes. We postulate that metabolic acidosis could have an important role to generate this sign.

  17. Molecular basis for PrimPol recruitment to replication forks by RPA.

    Science.gov (United States)

    Guilliam, Thomas A; Brissett, Nigel C; Ehlinger, Aaron; Keen, Benjamin A; Kolesar, Peter; Taylor, Elaine M; Bailey, Laura J; Lindsay, Howard D; Chazin, Walter J; Doherty, Aidan J

    2017-05-23

    DNA damage and secondary structures can stall the replication machinery. Cells possess numerous tolerance mechanisms to complete genome duplication in the presence of such impediments. In addition to translesion synthesis (TLS) polymerases, most eukaryotic cells contain a multifunctional replicative enzyme called primase-polymerase (PrimPol) that is capable of directly bypassing DNA damage by TLS, as well as repriming replication downstream of impediments. Here, we report that PrimPol is recruited to reprime through its interaction with RPA. Using biophysical and crystallographic approaches, we identify that PrimPol possesses two RPA-binding motifs and ascertained the key residues required for these interactions. We demonstrate that one of these motifs is critical for PrimPol's recruitment to stalled replication forks in vivo. In addition, biochemical analysis reveals that RPA serves to stimulate the primase activity of PrimPol. Together, these findings provide significant molecular insights into PrimPol's mode of recruitment to stalled forks to facilitate repriming and restart.

  18. Dynamic force microscopy with quartz tuning forks at high oscillation amplitudes

    International Nuclear Information System (INIS)

    Labardi, M

    2007-01-01

    Dynamic force microscopy (DFM) with the self-oscillator (SO) method allows reasonably high scanning rates even with high Q-factors of the resonant force sensor, typical of cantilevers in ultra-high vacuum and of quartz tuning forks. However, due to simpler interpretation of force spectroscopy measurements, small oscillation amplitudes (sub-nm level) are generally preferred. In applications like 'apertureless' scanning near-field optical microscopy (SNOM), oscillation amplitudes of the order of 5-10 nm are needed to increase optical sensitivity and to apply standard optical artefact suppression methods. This motivates the study of the behaviour of tuning forks driven at such high amplitudes, as compared to usual air-operated cantilevers. Both constant-excitation-amplitude (CE) and constant-oscillation-amplitude (CA) modes of SO-DFM are analysed, since the CA mode is more convenient for SNOM applications, denoting remarkable differences. In particular, possible instability effects, previously found in CE mode, are not anticipated for CA mode. It is shown how resonance and approach ('isophase') curves in both modes can be conveniently described in terms of the usual 'normalized frequency shift' γ and of a 'normalized gain' η, defined as a measurement of surface dissipation

  19. The ammonoids from the Three Forks Shale (Late Devonian of Montana

    Directory of Open Access Journals (Sweden)

    D. Korn

    2006-08-01

    Full Text Available The ammonoid fauna from the Late Devonian Three Forks Shale of Montana is revised. Six taxa were recognised, which belong to the genera Tornoceras, Pernoceras, Raymondiceras, Platyclymenia, Pleuroclymenia, and Carinoclymenia. The ammonoid assemblage suggests a stratigraphic position within the middle Famennian, most probably the Platyclymenia annulata Zone. The ammonoids display extreme septal crowding in intermediate as well as adult growth stages, which can be regarded as evidence for instable palaeoecological conditions during lifetime of the animals. Die Ammonoideenfauna aus dem oberdevonischen Three Forks Shale von Montana wird revidiert. Sechs Taxa werden unterschieden; sie gehören zu den Gattungen Tornoceras, Pernoceras, Raymondiceras, Platyclymenia, Pleuroclymenia und Carinoclymenia. Die Ammonoideen-Vergesellschaftung spricht für eine stratigraphische Position im mittleren Famennium, wahrscheinlich in der Platyclymenia annulata Zone. Die Ammonoideen zeigen auffällige Drängung der Septen in intermediären und adulten Wachstumsstadien, die als Hinweis auf instabile Lebensbedingungen für die Tiere gewertet werden kann. doi:10.1002/mmng.200600008

  20. Correlates of mercury in fish from lakes near Clyde Forks, Ontario, Canada

    International Nuclear Information System (INIS)

    Ethier, A.L.M.; Scheuhammer, A.M.; Bond, D.E.

    2008-01-01

    Subsurface soils near Clyde Forks, Ontario, Canada, can have naturally high concentrations of mercury (Hg) from local geological sources. To investigate Hg in local aquatic food webs, Hg was measured in fish dorsal muscle (mainly yellow perch [YP] and pumpkinseed sunfish [PS]) and surface sediments from 10 regional lakes. Water chemistry, along with fork length, weight, and stable isotopes (δ 15 N, δ 13 C, δ 34 S) in fish were also measured. No lake sediments had elevated (>0.3 μg/g dw) Hg, and average Hg concentrations in fish were not sufficiently high ( 13 C), and certain lake variables (e.g., pH for YP). PS with more pelagic feeding habits had higher δ 34 S and Hg than those with more littoral feeding habits. Potential biological linkages between fish Hg and δ 34 S, a parameter that may be related to the lake sulphate-reducing bacteria activity, requires further investigation. - Fish from lakes near a localized geological Hg source do not have elevated Hg concentrations

  1. Investigation of shallow groundwater contamination near East Fork Poplar Creek, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Carmichael, J.K.

    1989-01-01

    Alluvial soils of the flood plain of East Fork Poplar Creek in Oak Ridge, Tennessee, are contaminated with mercury and other metals, organic compounds, and radionuclides originating from the Y-12 Plant, a nuclear-processing facility located within the US Department of Energy's Oak Ridge Reservation. Observation wells were installed in the shallow aquifer of the flood plain, and water quality samples were collected to determine if contaminants are present in the shallow groundwater. Groundwater in the shallow aquifer occurs under water-table conditions. Recharge is primarily from precipitation and discharge is to East Fork Poplar Creek. Groundwater levels fluctuate seasonally in response to variations in recharge and evapotranspiration. During extremely dry periods, the water table drops below the base of the shallow aquifer in some flood-plain areas. Contaminants were found in water samples from several of the wells in concentrations which equaled or exceeded drinking-water standards established by the US Environmental Protection Agency are antimony, chromium, lead, mercury, selenium, phenols, and strontium-90. Total and dissolved uranium concentrations exceeded the analytical detection limit in nearly 70% of the wells in the flood plain. The results of water quality determinations demonstrate that elevated concentrations of most trace metals (and possibly organic compounds and radionuclides) were caused by contaminated sediments in the samples. The presence of contaminated sediment in samples is suspected to be the result of borehole contamination during well installation. 21 refs., 20 figs., 6 tabs

  2. Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.

    Science.gov (United States)

    Kurat, Christoph F; Yeeles, Joseph T P; Patel, Harshil; Early, Anne; Diffley, John F X

    2017-01-05

    The integrity of eukaryotic genomes requires rapid and regulated chromatin replication. How this is accomplished is still poorly understood. Using purified yeast replication proteins and fully chromatinized templates, we have reconstituted this process in vitro. We show that chromatin enforces DNA replication origin specificity by preventing non-specific MCM helicase loading. Helicase activation occurs efficiently in the context of chromatin, but subsequent replisome progression requires the histone chaperone FACT (facilitates chromatin transcription). The FACT-associated Nhp6 protein, the nucleosome remodelers INO80 or ISW1A, and the lysine acetyltransferases Gcn5 and Esa1 each contribute separately to maximum DNA synthesis rates. Chromatin promotes the regular priming of lagging-strand DNA synthesis by facilitating DNA polymerase α function at replication forks. Finally, nucleosomes disrupted during replication are efficiently re-assembled into regular arrays on nascent DNA. Our work defines the minimum requirements for chromatin replication in vitro and shows how multiple chromatin factors might modulate replication fork rates in vivo. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  3. Effects of Forecasted Climate Change on Stream Temperatures in the Nooksack River Basin

    Science.gov (United States)

    Truitt, S. E.; Mitchell, R. J.; Yearsley, J. R.; Grah, O. J.

    2017-12-01

    The Nooksack River in northwest Washington State provides valuable habitat for endangered salmon species, as such it is critical to understand how stream temperatures will be affected by forecasted climate change. The Middle and North Forks basins of the Nooksack are high-relief and glaciated, whereas the South Fork is a lower relief rain and snow dominated basin. Due to a moderate Pacific maritime climate, snowpack in the basins is sensitive to temperature increases. Previous modeling studies in the upper Nooksack basins indicate a reduction in snowpack and spring runoff, and a recession of glaciers into the 21st century. How stream temperatures will respond to these changes is unknown. We use the Distributed Hydrology Soil Vegetation Model (DHSVM) coupled with a glacier dynamics model and the River Basin Model (RBM) to simulate hydrology and stream temperature from present to the year 2100. We calibrate the DHSVM and RBM to the three forks in the upper 1550 km2 of the Nooksack basin, which contain an estimated 3400 hectares of glacial ice. We employ observed stream-temperature data collected over the past decade and hydrologic data from the four USGS streamflow monitoring sites within the basin and observed gridded climate data developed by Linveh et al. (2013). Field work was conducted in the summer of 2016 to determine stream morphology, discharge, and stream temperatures at a number of stream segments for the RBM calibration. We simulate forecast climate change impacts, using gridded daily downscaled data from global climate models of the CMIP5 with RCP4.5 and RCP8.5 forcing scenarios developed using the multivariate adaptive constructed analogs method (MACA; Abatzoglou and Brown, 2011). Simulation results project a trending increase in stream temperature as a result of lower snowmelt and higher air temperatures into the 21st century, especially in the lower relief, unglaciated South Fork basin.

  4. Hydrologic modeling of flood conveyance and impacts of historic overbank sedimentation on West Fork Black's Fork, Uinta Mountains, northeastern Utah, USA

    Science.gov (United States)

    Carson, Eric C.

    2006-05-01

    This study assesses historic overbank alluvial sedimentation along a low-gradient reach of West Fork Black's Fork in the northern Uinta Mountains, Utah. In this previously glaciated setting, an alluvial floodplain that is approximately 400 m wide by 1500 m long has been modified by the combined effects of valley morphometry and the recent history of clear-cut logging during the late 19th and early 20th Centuries. To quantify the effects on sedimentation and flow conveyance, three natural streambank exposures were sampled and analyzed for nuclear bomb fallout 137Cs. The distribution of 137Cs within the three profiles suggests that a remnant outwash terrace exerts a first-order control over the deposition of overbank alluvium. Upstream from a constriction in the floodplain caused by the terrace remnant, as much as 40 cm of overbank alluvium has been deposited since the beginning of clear-cut logging. Immediately downstream of that constriction, no evidence exists for any overbank sedimentation during that same period. Vibracore samples and Oakfield soil probe sampling throughout the study reach quantified the geographic extent and thicknesses of the historic alluvial package. Flood conveyance through the study area was modeled using the U.S. Army Corps of Engineers HEC-RAS modeling program. Model simulations were run for modern conditions (using surveyed topography) and for prehistoric conditions (using the modern topography less the historic alluvial package determined by 137Cs analyses). Model results indicate that the floodplain constriction caused a significant impediment to flood conveyance at even modest discharges during prehistoric conditions. This promoted ponding of floodwaters upstream of the constriction and deposition of alluvium. This has increased bank heights upstream of the constriction, to the point that under modern conditions 1- to 5-year recurrence interval floods are largely confined within the channel. These results confirm the validity of this

  5. Concentrations and loads of cadmium, lead, and zinc measured near the peak of the 1999 snowmelt-runoff hydrographs for 42 water-quality stations, Coeur d'Alene River basin, Idaho

    Science.gov (United States)

    Woods, Paul F.

    2000-01-01

    The Remedial Investigation/Feasibility Study conducted by the U.S. Environmental Protection Agency within the Spokane River Basin of northern Idaho and eastern Washington included extensive data-collection activities to determine the nature and extent of trace-element contamination within the basin. The U.S. Geological Survey designed and implemented synoptic sampling of the 1999 snowmelt-runoff event at 42 water- quality stations during the 1999 water year. The distribution of the 42 stations was as follows: North Fork Coeur d’Alene River and tributaries, 4 stations; South Fork Coeur d’Alene River, 13 stations; Canyon, Ninemile, and Pine Creeks, 4 stations each; other tributaries to South Fork Coeur d’Alene River, 10 stations; and main stem Coeur d’Alene River, 3 stations. The objective was to synoptically collect discharge and water-quality data in order to significantly improve the estimation of trace-element loads from multiple contributing source areas during the snowmelt-runoff event. Discharge and water-quality data were collected near the peak discharge during late May 1999. Each station was sampled for whole-water recoverable and dissolved concentrations and loads of cadmium, lead, and zinc.

  6. Fork2Farmer: Enabling Success of Small Farms through Partnerships with Well-Known Chefs and the Tourism Sector

    Science.gov (United States)

    Morais, Duarte; Jakes, Susan; Bowen, Becky; Lelekacs, Joanna Massey

    2017-01-01

    A team of economic development, local foods, and tourism specialists from North Carolina Cooperative Extension is pursuing an initiative titled Fork2Farmer. The goal is to increase visits to local farms and diversify farm income by leveraging the high visibility of well-known farm-to-table chefs who support local small farms. To do this, those…

  7. FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair

    Directory of Open Access Journals (Sweden)

    Zeina Kais

    2016-06-01

    Full Text Available BRCA1/2 proteins function in homologous recombination (HR-mediated DNA repair and cooperate with Fanconi anemia (FA proteins to maintain genomic integrity through replication fork stabilization. Loss of BRCA1/2 proteins results in DNA repair deficiency and replicative stress, leading to genomic instability and enhanced sensitivity to DNA-damaging agents. Recent studies have shown that BRCA1/2-deficient tumors upregulate Polθ-mediated alternative end-joining (alt-EJ repair as a survival mechanism. Whether other mechanisms maintain genomic integrity upon loss of BRCA1/2 proteins is currently unknown. Here we show that BRCA1/2-deficient tumors also upregulate FANCD2 activity. FANCD2 is required for fork protection and fork restart in BRCA1/2-deficient tumors. Moreover, FANCD2 promotes Polθ recruitment at sites of damage and alt-EJ repair. Finally, loss of FANCD2 in BRCA1/2-deficient tumors enhances cell death. These results reveal a synthetic lethal relationship between FANCD2 and BRCA1/2, and they identify FANCD2 as a central player orchestrating DNA repair pathway choice at the replication fork.

  8. The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast

    DEFF Research Database (Denmark)

    Larsen, Nicolai B; Sass, Ehud; Suski, Catherine

    2014-01-01

    Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus...... as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications....

  9. Computer Aided Design of the Link-Fork Head-Piston Assembly of the Kaplan Turbine with Solidworks

    Directory of Open Access Journals (Sweden)

    Camelia Jianu

    2010-10-01

    Full Text Available The paper presents the steps for 3D computer aided design (CAD of the link-fork head-piston assembly of the Kaplan turbine made in SolidWorks.The present paper is a tutorial for a Kaplan turbine assembly 3D geometry, which is dedicated to the Assembly design and Drawing Geometry and Drawing Annotation.

  10. MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context

    DEFF Research Database (Denmark)

    Bentsen, Iben Bach; Nielsen, Ida; Lisby, Michael

    2013-01-01

    location within the rDNA. We discover a pivotal role for the MRX (Mre11, Rad50, Xrs2) complex for fork integrity at RFBs, which differs from its acknowledged function in double-strand break processing. Consequently, in the absence of the MRX complex, single-stranded DNA (ssDNA) accumulates at the r...

  11. Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.

    Science.gov (United States)

    Cobb, Andrew M; Murray, Thomas V; Warren, Derek T; Liu, Yiwen; Shanahan, Catherine M

    2016-09-02

    The accumulation of prelamin A is linked to disruption of cellular homeostasis, tissue degeneration and aging. Its expression is implicated in compromised genome stability and increased levels of DNA damage, but to date there is no complete explanation for how prelamin A exerts its toxic effects. As the nuclear lamina is important for DNA replication we wanted to investigate the relationship between prelamin A expression and DNA replication fork stability. In this study we report that the expression of prelamin A in U2OS cells induced both mono-ubiquitination of proliferating cell nuclear antigen (PCNA) and subsequent induction of Pol η, two hallmarks of DNA replication fork stalling. Immunofluorescence microscopy revealed that cells expressing prelamin A presented with high levels of colocalisation between PCNA and γH2AX, indicating collapse of stalled DNA replication forks into DNA double-strand breaks. Subsequent protein-protein interaction assays showed prelamin A interacted with PCNA and that its presence mitigated interactions between PCNA and the mature nuclear lamina. Thus, we propose that the cytotoxicity of prelamin A arises in part, from it actively competing against mature lamin A to bind PCNA and that this destabilises DNA replication to induce fork stalling which in turn contributes to genomic instability.

  12. Reliability and responsiveness of a graduated tuning fork in immune mediated polyneuropathies. The Inflammatory Neuropathy Cause and Treatment (INCAT) Group

    NARCIS (Netherlands)

    I.S.J. Merkies (Ingemar); P.I.M. Schmitz (Paul); F.G.A. van der Meché (Frans); P.A. van Doorn (Pieter)

    2000-01-01

    textabstractThe interobserver and intraobserver reliability of the Rydel-Seiffer (RS) graduated tuning fork was evaluated in 113 patients with a clinically stable immune mediated polyneuropathy (83 patients who had had Guillain-Barre syndrome (GBS) in the past, 22 with

  13. The effect of real-time vibrotactile feedback delivered through an augmented fork on eating rate, satiation, and food intake

    NARCIS (Netherlands)

    Roel C. J. Hermans; Sander Hermsen; Eric Robinson; Suzanne Higgs; Jeana H. Frost; Monica Mars

    2017-01-01

    Eating rate is a basic determinant of appetite regulation, as people who eat more slowly feel sated earlier and eat less. Without assistance, eating rate is difficult to modify due to its automatic nature. In the current study, participants used an augmented fork that aimed to decelerate their rate

  14. Fork-join and data-driven execution models on multi-core architectures: Case study of the FMM

    KAUST Repository

    Amer, Abdelhalim; Maruyama, Naoya; Pericà s, Miquel; Taura, Kenjiro; Yokota, Rio; Matsuoka, Satoshi

    2013-01-01

    Extracting maximum performance of multi-core architectures is a difficult task primarily due to bandwidth limitations of the memory subsystem and its complex hierarchy. In this work, we study the implications of fork-join and data-driven execution

  15. NURE aerial gamma-ray and magnetic detail survey: W. Fork Sampoil River, Washington area. Final report

    International Nuclear Information System (INIS)

    1981-05-01

    Volume II contains the following: flight line base maps; magnetic contour maps; maps for uranium, thorium, potassium, uranium/thorium, thorium/potassium, uranium/potassium; multi-variate analysis maps; geochemical analysis maps; radiometric multiple parameter stacked profiles; magnetic and ancillary stacked profile data; histograms and statistical summaries for potassium, uranium, thorium, uranium/potassium, uranium/thorium, and thorium/potassium

  16. Flood-inundation maps for North Fork Salt Creek at Nashville, Indiana

    Science.gov (United States)

    Martin, Zachary W.

    2017-11-13

    Digital flood-inundation maps for a 3.2-mile reach of North Fork Salt Creek at Nashville, Indiana, were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The flood-inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science website at http://water.usgs.gov/osw/flood_inundation/, depict estimates of the areal extent and depth of flooding that correspond to selected water levels (stages) at the North Fork Salt Creek at Nashville, Ind., streamgage (USGS station number 03371650). Real-time stages at this streamgage may be obtained from the USGS National Water Information System at http://waterdata.usgs.gov/nwis or the National Weather Service (NWS) Advanced Hydrologic Prediction Service at http:/water.weather.gov/ahps/, which also shows observed USGS stages at the same site as the USGS streamgage (NWS site NFSI3).Flood profiles were computed for the stream reach by means of a one-dimensional, step-backwater hydraulic modeling software developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated using the current (2015) stage-discharge rating at the USGS streamgage 03371650, North Fork Salt Creek at Nashville, Ind. The hydraulic model was then used to compute 12 water-surface profiles for flood stages at 1-foot (ft) intervals, except for the highest profile of 22.9 ft, referenced to the streamgage datum ranging from 12.0 ft (the NWS “action stage”) to 22.9 ft, which is the highest stage of the current (2015) USGS stage-discharge rating curve and 1.9 ft higher than the NWS “major flood stage.” The simulated water-surface profiles were then combined with a geographic information system digital elevation model (derived from light detection and ranging data having a 0.98-ft vertical accuracy and 4.9-ft horizontal resolution) to delineate the area flooded at each stage.The availability of these maps, along with information regarding current stage from the USGS

  17. Captive Rearing Program for Salmon River Chinook Salmon, 2000 Project Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Venditti, David A.

    2002-04-01

    During 2000, the Idaho Department of Fish and Game (IDFG) continued to develop techniques to rear chinook salmon Oncorhynchus tshawytscha to sexual maturity in captivity and to monitor their reproductive performance under natural conditions. Eyed-eggs were collected to establish captive cohorts from three study streams and included 503 eyed-eggs from East Fork Salmon River (EFSR), 250 from the Yankee Fork Salmon River, and 304 from the West Fork Yankee Fork Salmon River (WFYF). After collection, the eyed-eggs were immediately transferred to the Eagle Fish Hatchery, where they were incubated and reared by family group. Juveniles collected the previous summer were PIT and elastomer tagged and vaccinated against vibrio Vibrio spp. and bacterial kidney disease before the majority (approximately 75%) were transferred to the National Marine Fisheries Service, Manchester Marine Experimental Station for saltwater rearing through sexual maturity. Smolt transfers included 158 individuals from the Lemhi River (LEM), 193 from the WFYF, and 372 from the EFSR. Maturing fish transfers from the Manchester facility to the Eagle Fish Hatchery included 77 individuals from the LEM, 45 from the WFYF, and 11 from the EFSR. Two mature females from the WFYF were spawned in captivity with four males in 2000. Only one of the females produced viable eggs (N = 1,266), which were placed in in-stream incubators by personnel from the Shoshone-Bannock Tribe. Mature adults (N = 70) from the Lemhi River were released into Big Springs Creek to evaluate their reproductive performance. After release, fish distributed themselves throughout the study section and displayed a progression of habitat associations and behavior consistent with progressing maturation and the onset of spawning. Fifteen of the 17 suspected redds spawned by captive-reared parents in Big Springs Creek were hydraulically sampled to assess survival to the eyed stage of development. Eyed-eggs were collected from 13 of these, and

  18. Frequency shift, damping, and tunneling current coupling with quartz tuning forks in noncontact atomic force microscopy

    Science.gov (United States)

    Nony, Laurent; Bocquet, Franck; Para, Franck; Loppacher, Christian

    2016-09-01

    A combined experimental and theoretical approach to the coupling between frequency-shift (Δ f ) , damping, and tunneling current (It) in combined noncontact atomic force microscopy/scanning tunneling microscopy using quartz tuning forks (QTF)-based probes is reported. When brought into oscillating tunneling conditions, the tip located at the QTF prong's end radiates an electromagnetic field which couples to the QTF prong motion via its piezoelectric tensor and loads its electrodes by induction. Our approach explains how those It-related effects ultimately modify the Δ f and the damping measurements. This paradigm to the origin of the coupling between It and the nc-AFM regular signals relies on both the intrinsic piezoelectric nature of the quartz constituting the QTF and its electrodes design.

  19. Piezoelectric tuning fork biosensors for the quantitative measurement of biomolecular interactions

    International Nuclear Information System (INIS)

    Gonzalez, Laura; Maria Benito, Angel; Puig-Vidal, Manel; Otero, Jorge; Rodrigues, Mafalda; Pérez-García, Lluïsa

    2015-01-01

    The quantitative measurement of biomolecular interactions is of great interest in molecular biology. Atomic force microscopy (AFM) has proved its capacity to act as a biosensor and determine the affinity between biomolecules of interest. Nevertheless, the detection scheme presents certain limitations when it comes to developing a compact biosensor. Recently, piezoelectric quartz tuning forks (QTFs) have been used as laser-free detection sensors for AFM. However, only a few studies along these lines have considered soft biological samples, and even fewer constitute quantified molecular recognition experiments. Here, we demonstrate the capacity of QTF probes to perform specific interaction measurements between biotin–streptavidin complexes in buffer solution. We propose in this paper a variant of dynamic force spectroscopy based on representing adhesion energies E (aJ) against pulling rates v (nm s"–"1). Our results are compared with conventional AFM measurements and show the great potential of these sensors in molecular interaction studies. (paper)

  20. Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork.

    Science.gov (United States)

    Choe, Katherine N; Moldovan, George-Lucian

    2017-02-02

    Proliferating cell nuclear antigen (PCNA) lies at the center of the faithful duplication of eukaryotic genomes. With its distinctive doughnut-shaped molecular structure, PCNA was originally studied for its role in stimulating DNA polymerases. However, we now know that PCNA does much more than promote processive DNA synthesis. Because of the complexity of the events involved, cellular DNA replication poses major threats to genomic integrity. Whatever predicament lies ahead for the replication fork, PCNA is there to orchestrate the events necessary to handle it. Through its many protein interactions and various post-translational modifications, PCNA has far-reaching impacts on a myriad of cellular functions. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. [History of the tuning fork. II: Evolution of the classical experiments by Weber, Rinne and Schwabach].

    Science.gov (United States)

    Feldmann, H

    1997-05-01

    Since the 17th centrury it was known that sounds could be perceived via air conduction and bone conduction and that this provided a means of differentiating between hearing disorders located in the middle ear and those located in the acoustic nerve. For a long time to come, however, there was no need for such a differential diagnosis. After the invention of the tuning fork in 1711 this instrument had soon become widely used in music, but it took well over 100 years until it was introduced into physiology and otology. FROM DIRECTIONAL HEARING TO WEBER'S TEST: J. B. Venturi, a physicist in Modena, Italy, in 1802 had shown that the perception of the direction from which a sound is coming is governed by the fact that one ear is hit by the sound more intensely than the other ear. C. T. Tourtual, a physician in Münster, Germany, demonstrated in 1827 that this also holds true for sound conducted via the skull bones. He used a watch as sound source. He found that occlusion of both ear canals would increase the sensation in both ears equally, but that occlusion of only one ear would increase the sensation only in the occluded ear, thus giving the impression that the sound were coming from that side. He was interested in a comparison between vision and audition, and he concluded that with regard to recognizing the direction of a sensory signal vision was superior to audition. In the same year 1827 C. Wheatstone, a physicist in London, investigating the mode of vibration of the tympanic membrane and using a tuning fork found the same phenomena as Tourtual and some more effects. E. H. Weber, an anatomist and physiologist in Leipzig, Germany, described the very same phenomena as Tourtual and Wheatstone once more in 1834. He wanted to prove that airborne sound is perceived by the vestibulum and the semicircular canals, bone conducted sound by the cochlea. None of these investigators was thinking of a clinical use of their findings and made no such suggestion. E. Schmalz, an

  2. The phase interrogation method for optical fiber sensor by analyzing the fork interference pattern

    Science.gov (United States)

    Lv, Riqing; Qiu, Liqiang; Hu, Haifeng; Meng, Lu; Zhang, Yong

    2018-02-01

    The phase interrogation method for optical fiber sensor is proposed based on the fork interference pattern between the orbital angular momentum beam and plane wave. The variation of interference pattern with phase difference between the two light beams is investigated to realize the phase interrogation. By employing principal component analysis method, the features of the interference pattern can be extracted. Moreover, the experimental system is designed to verify the theoretical analysis, as well as feasibility of phase interrogation. In this work, the Mach-Zehnder interferometer was employed to convert the strain applied on sensing fiber to the phase difference between the reference and measuring paths. This interrogation method is also applicable for the measurements of other physical parameters, which can produce the phase delay in optical fiber. The performance of the system can be further improved by employing highlysensitive materials and fiber structures.

  3. Quartz enhanced photoacoustic spectroscopy based trace gas sensors using different quartz tuning forks.

    Science.gov (United States)

    Ma, Yufei; Yu, Guang; Zhang, Jingbo; Yu, Xin; Sun, Rui; Tittel, Frank K

    2015-03-27

    A sensitive trace gas sensor platform based on quartz-enhanced photoacoustic spectroscopy (QEPAS) is reported. A 1.395 μm continuous wave (CW), distributed feedback pigtailed diode laser was used as the excitation source and H2O was selected as the target analyte. Two kinds of quartz tuning forks (QTFs) with a resonant frequency (f0) of 30.72 kHz and 38 kHz were employed for the first time as an acoustic wave transducer, respectively for QEPAS instead of a standard QTF with a f0 of 32.768 kHz. The QEPAS sensor performance using the three different QTFs was experimentally investigated and theoretically analyzed. A minimum detection limit of 5.9 ppmv and 4.3 ppmv was achieved for f0 of 32.768 kHz and 30.72 kHz, respectively.

  4. Quartz Enhanced Photoacoustic Spectroscopy Based Trace Gas Sensors Using Different Quartz Tuning Forks

    Directory of Open Access Journals (Sweden)

    Yufei Ma

    2015-03-01

    Full Text Available A sensitive trace gas sensor platform based on quartz-enhanced photoacoustic spectroscopy (QEPAS is reported. A 1.395 μm continuous wave (CW, distributed feedback pigtailed diode laser was used as the excitation source and H2O was selected as the target analyte. Two kinds of quartz tuning forks (QTFs with a resonant frequency (f0 of 30.72 kHz and 38 kHz were employed for the first time as an acoustic wave transducer, respectively for QEPAS instead of a standard QTF with a f0 of 32.768 kHz. The QEPAS sensor performance using the three different QTFs was experimentally investigated and theoretically analyzed. A minimum detection limit of 5.9 ppmv and 4.3 ppmv was achieved for f0 of 32.768 kHz and 30.72 kHz, respectively.

  5. Modulation of mutagenesis in eukaryotes by DNA replication fork dynamics and quality of nucleotide pools

    Science.gov (United States)

    Waisertreiger, Irina S.-R.; Liston, Victoria G.; Menezes, Miriam R.; Kim, Hyun-Min; Lobachev, Kirill S.; Stepchenkova, Elena I.; Tahirov, Tahir H.; Rogozin, Igor B.; Pavlov, Youri. I.

    2014-01-01

    The rate of mutations in eukaryotes depends on a plethora of factors and is not immediately derived from the fidelity of DNA polymerases (Pols). Replication of chromosomes containing the anti-parallel strands of duplex DNA occurs through the copying of leading and lagging strand templates by a trio of Pols α, δ and ε, with the assistance of Pol ζ and Y-family Pols at difficult DNA template structures or sites of DNA damage. The parameters of the synthesis at a given location are dictated by the quality and quantity of nucleotides in the pools, replication fork architecture, transcription status, regulation of Pol switches, and structure of chromatin. The result of these transactions is a subject of survey and editing by DNA repair. PMID:23055184

  6. Waste management plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Plant Landfill V, and restore the affected floodplain. The waste management plan addresses management and disposition of all wastes generated during the LEFPC remedial action. Most of the solid wastes will be sanitary or construction/demolition wastes and will be disposed of at existing Y- 12 facilities. Some small amounts of hazardous waste are anticipated, along with possible low-level or mixed wastes (> 35 pCi/g). Liquid wastes will be generated which will be sanitary and capable of being disposed of at the Oak Ridge Sewage Treatment Plant, except sanitary sewage.

  7. Waste management plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Plant Landfill V, and restore the affected floodplain. The waste management plan addresses management and disposition of all wastes generated during the LEFPC remedial action. Most of the solid wastes will be sanitary or construction/demolition wastes and will be disposed of at existing Y- 12 facilities. Some small amounts of hazardous waste are anticipated, along with possible low-level or mixed wastes (> 35 pCi/g). Liquid wastes will be generated which will be sanitary and capable of being disposed of at the Oak Ridge Sewage Treatment Plant, except sanitary sewage

  8. Mercury Remediation Technology Development for Lower East Fork Poplar Creek - FY 2015 Progress Report

    International Nuclear Information System (INIS)

    Peterson, Mark J.; Smith, John; Eller, Virginia; DeRolph, Christopher R.

    2016-01-01

    Mercury remediation is a high priority for the US Department of Energy (DOE) Oak Ridge Office of Environmental Management (OREM) because of large historical losses of mercury within buildings and to soils and surface waters at the Y-12 National Security Complex (Y-12). Because of the extent of mercury losses and the complexities of mercury transport and fate in the downstream environment, the success of conventional options for mercury remediation in lower East Fork Poplar Creek (EFPC) is uncertain. A phased, adaptive management approach to remediation of surface water includes mercury treatment actions at Y-12 in the short-term and research and technology development (TD) to evaluate longer-term solutions in the downstream environment (US Department of Energy 2014b).

  9. Mercury Remediation Technology Development for Lower East Fork Poplar Creek - FY 2015 Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Mayes, Melanie [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Johs, Alexander [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Poteat, Monica D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Smith, John [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Mehlhorn, Tonia [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Lester, Brian [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Morris, Jesse [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Lowe, Kenneth [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Dickson, Johnbull O. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Eller, Virginia [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division

    2016-04-01

    Mercury remediation is a high priority for the US Department of Energy (DOE) Oak Ridge Office of Environmental Management (OREM) because of large historical losses of mercury within buildings and to soils and surface waters at the Y-12 National Security Complex (Y-12). Because of the extent of mercury losses and the complexities of mercury transport and fate in the downstream environment, the success of conventional options for mercury remediation in lower East Fork Poplar Creek (EFPC) is uncertain. A phased, adaptive management approach to remediation of surface water includes mercury treatment actions at Y-12 in the short-term and research and technology development (TD) to evaluate longer-term solutions in the downstream environment (US Department of Energy 2014b).

  10. Interface circuit for a multiple-beam tuning-fork gyroscope with high quality factors

    Science.gov (United States)

    Wang, Ren

    This research work presents the design, theoretical analysis, fabrication, interface electronics, and experimental results of a Silicon-On-Insulator (SOI) based Multiple-Beam Tuning-Fork Gyroscope (MB-TFG). Based on a numerical model of Thermo-Elastic Damping (TED), a Multiple-Beam Tuning-Fork Structure (MB-TFS) is designed with high Quality factors (Qs) in its two operation modes. A comprehensive theoretical analysis of the MB-TFG design is conducted to relate the design parameters to its operation parameters and further performance parameters. In conjunction with a mask that defines the device through trenches to alleviate severe fabrication effect on anchor loss, a simple one-mask fabrication process is employed to implement this MB-TFG design on SOI wafers. The fabricated MB-TFGs are tested with PCB-level interface electronics and a thorough comparison between the experimental results and a theoretical analysis is conducted to verify the MB-TFG design and accurately interpret the measured performance. The highest measured Qs of the fabricated MB-TFGs in vacuum are 255,000 in the drive-mode and 103,000 in the sense-mode, at a frequency of 15.7kHz. Under a frequency difference of 4Hz between the two modes (operation frequency is 16.8kHz) and a drive-mode vibration amplitude of 3.0um, the measured rate sensitivity is 80mVpp/°/s with an equivalent impedance of 6MQ. The calculated overall rate resolution of this device is 0.37/hrhiElz, while the measured Angle Random Walk (ARW) and bias instability are 6.67°/'vhr and 95°/hr, respectively.

  11. Tennessee's East Fork Poplar Creek: A biological monitoring and abatement program

    International Nuclear Information System (INIS)

    Halbrook, R.S.; Loar, J.M.; Adams, S.M.; Black, M.C.; Boston, H.L.; Greeley, M.S. Jr.; Hill, W.R.; Hinzman, R.L.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Schilling, E.M.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Gatz, A.J.

    1991-01-01

    On May 1985, a Biological Monitoring Program was developed for East Fork Poplar Creek (EFPC) in eastern Tennessee, United States. This stream originates within the Oak Ridge Y-12 Plant that produces nuclear weapons components for the Department of Energy. Water and sediment in the stream contain metals, organic chemicals, and radionuclides from releases that have occurred over the past 45 years. The creek also receives urban and some agricultural runoff and effluent from the City of Oak Ridge's Wastewater Treatment Facility (WTF). The biological monitoring program includes four major tasks: (1) ambient toxicity testing: (2) bioaccumulation studies; (3) biological indicator studies; and (4) ecological monitoring of stream communities, including periphyton, benthic macroinvertebrates, and fish. Biological conditions are monitored at six sites on EFPC ranging from kilometer 24.4 near the headwaters to kilometer 6.3 near the month. A site on Brushy Fork, A stream just north of Oak Ridge, is used as reference. Ambient (instream) toxicity was monitored through the use of 7-day static-renewal tests that measured the survival and growth of fathead minnow (Pimephales promelas) larvae and the survival and reproduction of a microstrustacean (Ceriodaphnia dubia). Full-strength water from EFPC within the Y-12 Plant boundary was frequently toxic to Ceriodaphnia, but less frequently toxic to the minnow larvae. Chlorine has been identified as an important toxicant in upper EFPC. Water samples from six sites in EFPC downstream from the Y-12 Plant boundary were tested eight times with both species during a 2-year period (October, 1986 through October, 1988). These sites were ranked by the number of times they were ''best'' or ''worst'' for each species. Water samples collected for use in the ambient toxicity tests were routinely analyzed for conductivity, pH, alkalinity, hardness, total residual and free chlorine, and temperature

  12. Shallow stratigraphy of the Skagit River Delta, Washington, derived from sediment cores

    Science.gov (United States)

    Grossman, Eric E.; George, Douglas A.; Lam, Angela

    2011-01-01

    Sedimentologic analyses of 21 sediment cores, ranging from 0.4 to 9.6 m in length, reveal that the shallow geologic framework of the Skagit River Delta, western Washington, United States, has changed significantly since 1850. The cores collected from elevations of 3.94 to -2.41 m (relative to mean lower low water) along four cross-shore transects between the emergent marsh and delta front show relatively similar environmental changes across an area spanning ~75 km2. Offshore of the present North Fork Skagit River and South Fork Skagit River mouths where river discharge is focused by diked channels through the delta, the entire 5–7-km-wide tidal flats are covered with 1–2 m of cross-bedded medium-to-coarse sands. The bottoms of cores, collected in these areas are composed of mud. A sharp transition from mud to a cross-bedded sand unit indicates that the tidal flats changed abruptly from a calm environment to an energetic one. This is in stark contrast to the Martha's Bay tidal flats north of the Skagit Bay jetty that was completed in the 1940s to protect the newly constructed Swinomish Channel from flooding and sedimentation. North of the jetty, mud ranging from 1 to 2 m thick drapes a previously silt- and sand-rich tidal flat. The silty sand is a sediment facies that would be expected there where North Fork Skagit River sedimentation occurred prior to jetty emplacement. This report describes the compositional and textural properties of the sediment cores by using geophysical, photographic, x-radiography, and standard sediment grain-size and carbon-analytical methods. The findings help to characterize benthic habitat structure and sediment transport processes and the environmental changes that have occurred across the nearshore of the Skagit River Delta. The findings will be useful for quantifying changes to nearshore marine resources, including impacts resulting from diking, river-delta channelization, shoreline development, and natural variations in fluvial

  13. Water-quality trends for selected sampling sites in the upper Clark Fork Basin, Montana, water years 1996-2010

    Science.gov (United States)

    Sando, Steven K.; Vecchia, Aldo V.; Lorenz, David L.; Barnhart, Elliott P.

    2014-01-01

    A large-scale trend analysis was done on specific conductance, selected trace elements (arsenic, cadmium, copper, iron, lead, manganese, and zinc), and suspended-sediment data for 22 sites in the upper Clark Fork Basin for water years 1996–2010. Trend analysis was conducted by using two parametric methods: a time-series model (TSM) and multiple linear regression on time, streamflow, and season (MLR). Trend results for 1996–2010 indicate moderate to large decreases in flow-adjusted concentrations (FACs) and loads of copper (and other metallic elements) and suspended sediment in Silver Bow Creek upstream from Warm Springs. Deposition of metallic elements and suspended sediment within Warm Springs Ponds substantially reduces the downstream transport of those constituents. However, mobilization of copper and suspended sediment from floodplain tailings and stream banks in the Clark Fork reach from Galen to Deer Lodge is a large source of metallic elements and suspended sediment, which also affects downstream transport of those constituents. Copper and suspended-sediment loads mobilized from within this reach accounted for about 40 and 20 percent, respectively, of the loads for Clark Fork at Turah Bridge (site 20); whereas, streamflow contributed from within this reach only accounted for about 8 percent of the streamflow at Turah Bridge. Minor changes in FACs and loads of copper and suspended sediment are indicated for this reach during 1996–2010. Clark Fork reaches downstream from Deer Lodge are relatively smaller sources of metallic elements than the reach from Galen to Deer Lodge. In general, small decreases in loads and FACs of copper and suspended sediment are indicated for Clark Fork sites downstream from Deer Lodge during 1996–2010. Thus, although large decreases in FACs and loads of copper and suspended sediment are indicated for Silver Bow Creek upstream from Warm Springs, those large decreases are not translated to the more downstream reaches largely

  14. Characterize and Quantify Residual Steelhead in the Clearwater River, Idaho, 1999-2000 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Brostrom, Jody K. (US Fish and Wildlife Service, Idaho Fishery Resource Office, Ahsahka, ID)

    2006-08-01

    During 1999-2002 we determined whether size at release and release site influenced emigration success and survival of hatchery steelhead smolts raised at Dworshak National Fish Hatchery and released into the Clearwater River drainage. We marked 4,500 smolts each year with Passive Integrated Transponder Tags (PIT-tags) which enabled us to track emigration and estimate survival through mainstem Snake and Columbia river dams. Hatchery steelhead raised in System I freshwater were significantly smaller than those raised in warmer System II re-use water (196 mm, 206 mm, 198 mm and 201 mm System I; 215 mm, 213 mm, 206 mm and 209 mm System II). However, there was no significant difference in detection rates to mainstem observation sites between the two groups (65%, 58%, 78% and 55% System I; 69%, 59%, 74% and 53% System II). Survival estimates to Lower Granite Dam were also not significant between the two groups (72%, 81%, 80% and 77% System I; 77%, 79%, 77%, and 72% System II). Smolts less than 180 mm FL were less likely to be detected than larger smolts. Hatchery steelhead smolts released into Clear Creek, the South Fork Clearwater River and the Clearwater River at Dworshak National Fish Hatchery had significantly different lengths each year, but there was no discernible pattern due to random egg takes and rearing systems. Detection rates to mainstem observation sites for smolts released into Clear Creek were significantly less than the other two groups in all years except 2002 (62%, 57%, 71%, and 57% Clear Creek; 68%, 63%, 73% and 61% South Fork Clearwater River; 70%, 59%, 78% and 55% Clearwater River). However, survival rates to Lower Granite Dam were not significantly different (73%, 65%, 78%, and 77% Clear Creek; 79%, 72%, 79% and 76% South Fork Clearwater River; 81%, 76%, 80% and 83% Clearwater River). Similar to the size at release group, smolts less than 180 mm FL were less likely to get detected than larger smolts. Smolts from both size at release and release

  15. Escapement and Productivity of Spring Chinook and Summer Steelhead in the John Day River Basin, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, Wayne

    2007-04-01

    The objectives are: (1) Estimate number and distribution of spring Chinook salmon Oncorhynchus tshawytscha redds and spawners in the John Day River subbasin; and (2) Estimate smolt-to-adult survival rates (SAR) and out-migrant abundance for spring Chinook and summer steelhead O. mykiss and life history characteristics of summer steelhead. Spawning ground surveys for spring (stream-type) Chinook salmon were conducted in four main spawning areas (Mainstem, Middle Fork, North Fork, and Granite Creek System) and seven minor spawning areas (South Fork, Camas Creek, Desolation Creek, Trail Creek, Deardorff Creek, Clear Creek, and Big Creek) in the John Day River basin during August and September of 2005. Census surveys included 298.2 river kilometers (88.2 rkm within index, 192.4 rkm additional within census, and 17.6 rkm within random survey areas) of spawning habitat. We observed 902 redds and 701 carcasses including 227 redds in the Mainstem, 178 redds in the Middle Fork, 420 redds in the North Fork, 62 redds in the Granite Creek System, and 15 redds in Desolation Creek. Age composition of carcasses sampled for the entire basin was 1.6% age 3, 91.2% age 4, and 7.1% age 5. The sex ratio was 57.4% female and 42.6% male. Significantly more females than males were observed in the Granite Creek System. During 2005, 82.3% of female carcasses sampled had released all of their eggs. Significantly more pre-spawn mortalities were observed in Granite Creek. Nine (1.3%) of 701 carcasses were of hatchery origin. Of 298 carcasses examined, 4.0% were positive for the presence of lesions. A significantly higher incidence of gill lesions was found in the Granite Creek System when compared to the rest of the basin. Of 114 kidney samples tested, two (1.8%) had clinical BKD levels. Both infected fish were age-4 females in the Middle Fork. All samples tested for IHNV were negative. To estimate spring Chinook and summer steelhead smolt-to-adult survival (SAR) we PIT tagged 5,138 juvenile

  16. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils: LEFPC appendices, volume 1, appendix I-IV

    International Nuclear Information System (INIS)

    1994-09-01

    This document contains Appendix I-IV for the pilot-scale thermal treatment of lower East Fork Poplar Creek floodplain soils. Included are calibration records; quality assurance; soils characterization; pilot scale trial runs

  17. Monitoring Fine Sediment; Grande Ronde and John Day Rivers, 2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rhodes, Jonathan J.; Greene, M. Jonas; Purser, Michael D. (Columbia River Inter-Tribal Fish Commission, Portland, OR)

    2001-01-01

    Fine sediment in spawning substrate has a major effect on salmon survival from egg to smolt. Basin-wide restoration plans have established targets for fine sediment levels in spawning habitat. The project was initiated to monitor surface fine sediment levels and overwinter intrusion of fine sediment in spring chinook salmon spawning habitat in the North Fork John Day (NFJDR) and Grande Ronde Rivers, for five years. The project is also investigating the potential relationship between surface fine levels and overwinter sedimentation. It will provide data to assess trends in substrate conditions in monitored reaches and whether trends are consistent with efforts to improve salmon habitat conditions. The data on the magnitude of overwinter sedimentation will also be used to estimate salmon survival from egg to emergence. In Sept. 1998, 1999, and Aug. 2000, sites for monitoring overwinter sedimentation were established in salmon spawning habitat in the upper Grande Ronde River, Catherine Creek (a Grande Ronde tributary), the North Fork John Day River (NFJDR), and Granite Creek (a NFJDR tributary). Surface fine sediment levels were measured in these reaches via the grid method and visually estimated to test the relative accuracy of these two methods. In 1999 and 2000, surface fine sediment was also estimated via pebble counts at selected reaches to allow comparison of results among the methods. Overwintering substrate samples were collected in April 1999 and April-May 2000 to estimate the amount of overwinter sedimentation in clean gravels in spawning habitat. Monitoring methods and locations are described.

  18. The Long-Term Effects of Large Wood Placement on Salmonid Habitat in East Fork Mill Creek, Redwood National and State Park, California

    Science.gov (United States)

    Rodriguez, D. L.; Stubblefield, A. P.

    2017-12-01

    The conservation and recovery of anadromous salmonids (Oncorhynchus sp.) depend on stream restoration and protection of freshwater habitats. Instream large wood dictates channel morphology, increase retention of terrestrial inputs such as organic matter, nutrients and sediment, and enhances the quality of fish habitat. Historic land use/land cover changes have resulted in aquatic systems devoid of this component. Restoration by placement of large wood jams is intended to restore physical and biological processes. An important question for scientists and managers, in addition to the initial effectiveness of restoration, is the persistence and fate of this type of project. In this study we compare channel change and large wood attributes on the East Fork of Mill Creek, a tributary of the Smith River in northern California, eight years after a major instream wood placement effort took place. Our results are compared with previously published data from before and one year after the restoration. Preliminary results suggest the dramatic increase in spawning gravel abundance and large wood accumulation observed in the earlier study have persisted. From 2008 to 2016 a reduction in median sediment size, ranging from 103-136 percent, has been observed in a majority of the sites. The sites have continued to grow in size and influence by racking floating wood from upstream and destabilizing proximate banks of riparian alder, increasing both instream large wood volume (5-196 %) and floodplain connectivity. Preliminary results also show a decrease in residual pool depth and an increase in pool length which may be attributed to floodplain connectivity. Changes to the following attributes are evaluated: 1) wood loading (total site wood volume, total wood volume in active channel, and wood piece count); 2) percent pool cover by large wood; 3) residual pool depth; 4) upstream sediment aggradation; 5) floodplain connectivity; and 6) mean sediment size directly above and below large

  19. Antecedent Rivers

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 8. Antecedent Rivers - Ganga Is Older Than Himalaya. K S Valdiya. General Article Volume 1 Issue 8 August 1996 pp 55-63. Fulltext. Click here to view fulltext PDF. Permanent link: https://www.ias.ac.in/article/fulltext/reso/001/08/0055-0063 ...

  20. RIVER STATE

    African Journals Online (AJOL)

    principals randomly selected from one hundred secondary schools in Cross River State. The data collected ... There was no siyriificant influerlce of gender on principals' leadership styles effectiveness. ... result of the cultural stereotyping of males and females by .... schools were single sex boys, another 10 were single sex ...

  1. Sediment discharge of the rivers of Catalonia, NE Spain, and the influence of human impacts

    Science.gov (United States)

    Liquete, Camino; Canals, Miquel; Ludwig, Wolfgang; Arnau, Pedro

    2009-03-01

    SummaryThe environmental and anthropogenic factors controlling sediment delivery to the sea are numerous, intricate and usually difficult to quantify. Mediterranean watersheds are historically amongst the most heavily impacted by human activities in the world. This study analyzes some of these factors for nine river systems from Catalonia, NE Spain, that open into the Northwestern Mediterranean Sea, and discusses the results obtained from sediment yield models and sediment concentration data series. General models indicate that the natural suspended sediment yield by individual Catalan rivers ranged within a fork from 94 to 621 t km -2 yr -1. Such a sediment yield would be noticeably reduced (moving the fork to 7-148 t km -2 yr -1) because of lithological factors and direct anthropogenic and, possibly, climatic impacts. Damming, water extraction and urbanization appear as the most important direct anthropogenic impacts in Catalonia. Water discharge and sediment concentration measurements by basin authorities provide much lower sediment yield estimations, from 0.4 to 19.8 t km -2 yr -1, which is probably due to the lack of measured sediment loads during flood events, as it is the case in many other Mediterranean rivers. The Catalan watersheds have some of the smallest runoff values amongst Mediterranean rivers. Of the nine river systems studied, water discharge tends to decrease in two and to increase in one. The other six river systems do not show any clear tendency. Related to climatic parameters, temperature raised in all the watersheds between 1961 and 1990, while precipitation did not show significant trends.

  2. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells

    DEFF Research Database (Denmark)

    Ahuja, Akshay K.; Jodkowska, Karolina; Teloni, Federico

    2016-01-01

    Embryonic stem cells (ESCs) represent a transient biological state, where pluripotency is coupled with fast proliferation. ESCs display a constitutively active DNA damage response (DDR), but its molecular determinants have remained elusive. Here we show in cultured ESCs and mouse embryos that H2AX...... these marks of replication stress do not impair the mitotic process and are rapidly lost at differentiation onset. Delaying the G1/S transition in ESCs allows formation of 53BP1 nuclear bodies and suppresses ssDNA accumulation, fork slowing and reversal in the following S-phase. Genetic inactivation of fork...... slowing and reversal leads to chromosomal breakage in unperturbed ESCs. We propose that rapid cell cycle progression makes ESCs dependent on effective replication-coupled mechanisms to protect genome integrity....

  3. Aerial gamma ray and magnetic survey: Minnesota Project, Grand Forks quadrangle of Minnesota/North Dakota. Final report

    International Nuclear Information System (INIS)

    1979-12-01

    The Grand Forks 1:250,000 scale quadrangle of Minnesota and North Dakota is almost everywhere covered with Wisconsin age glacial deposits (drift, lake sediments, etc.) of variable thickness. Where exposed, bedrock is Late Cretaceous age marine deposits. There are no uranium deposits (or occurrences) known within the quadrangle. Seventy-eight (78) groups of uranium samples were defined as anomalies and are discussed briefly in this report. None of them are considered significant

  4. Confirmatory Sampling and Analysis Plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-12-01

    This document describes the organization, strategy, and procedures to be used to confirm that mercury concentrations in soils in the remediated areas are statistically less than, or equal to, the cleanup standard of 400 ppm. It focuses on confirming the cleanup of the stretch of the Lower East Fork Popular Creed flowing from Lake Reality at the Y-12 Plant, through the City of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation and its associated flood plain

  5. Strand-Specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast.

    Science.gov (United States)

    Yu, Chuanhe; Gan, Haiyun; Zhang, Zhiguo

    2018-01-01

    DNA replication initiates at DNA replication origins after unwinding of double-strand DNA(dsDNA) by replicative helicase to generate single-stranded DNA (ssDNA) templates for the continuous synthesis of leading-strand and the discontinuous synthesis of lagging-strand. Therefore, methods capable of detecting strand-specific information will likely yield insight into the association of proteins at leading and lagging strand of DNA replication forks and the regulation of leading and lagging strand synthesis during DNA replication. The enrichment and Sequencing of Protein-Associated Nascent DNA (eSPAN), which measure the relative amounts of proteins at nascent leading and lagging strands of DNA replication forks, is a step-wise procedure involving the chromatin immunoprecipitation (ChIP) of a protein of interest followed by the enrichment of protein-associated nascent DNA through BrdU immunoprecipitation. The isolated ssDNA is then subjected to strand-specific sequencing. This method can detect whether a protein is enriched at leading or lagging strand of DNA replication forks. In addition to eSPAN, two other strand-specific methods, (ChIP-ssSeq), which detects potential protein-ssDNA binding and BrdU-IP-ssSeq, which can measure synthesis of both leading and lagging strand, were developed along the way. These methods can provide strand-specific and complementary information about the association of the target protein with DNA replication forks as well as synthesis of leading and lagging strands genome wide. Below, we describe the detailed eSPAN, ChIP-ssSeq, and BrdU-IP-ssSeq protocols.

  6. Confirmatory Sampling and Analysis Plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    This document describes the organization, strategy, and procedures to be used to confirm that mercury concentrations in soils in the remediated areas are statistically less than, or equal to, the cleanup standard of 400 ppm. It focuses on confirming the cleanup of the stretch of the Lower East Fork Popular Creed flowing from Lake Reality at the Y-12 Plant, through the City of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation and its associated flood plain.

  7. RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together.

    Science.gov (United States)

    Seeber, Andrew; Hegnauer, Anna Maria; Hustedt, Nicole; Deshpande, Ishan; Poli, Jérôme; Eglinger, Jan; Pasero, Philippe; Gut, Heinz; Shinohara, Miki; Hopfner, Karl-Peter; Shimada, Kenji; Gasser, Susan M

    2016-12-01

    The Mre11-Rad50-Xrs2 (MRX) complex is related to SMC complexes that form rings capable of holding two distinct DNA strands together. MRX functions at stalled replication forks and double-strand breaks (DSBs). A mutation in the N-terminal OB fold of the 70 kDa subunit of yeast replication protein A, rfa1-t11, abrogates MRX recruitment to both types of DNA damage. The rfa1 mutation is functionally epistatic with loss of any of the MRX subunits for survival of replication fork stress or DSB recovery, although it does not compromise end-resection. High-resolution imaging shows that either the rfa1-t11 or the rad50Δ mutation lets stalled replication forks collapse and allows the separation not only of opposing ends but of sister chromatids at breaks. Given that cohesin loss does not provoke visible sister separation as long as the RPA-MRX contacts are intact, we conclude that MRX also serves as a structural linchpin holding sister chromatids together at breaks. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Fork-join and data-driven execution models on multi-core architectures: Case study of the FMM

    KAUST Repository

    Amer, Abdelhalim

    2013-01-01

    Extracting maximum performance of multi-core architectures is a difficult task primarily due to bandwidth limitations of the memory subsystem and its complex hierarchy. In this work, we study the implications of fork-join and data-driven execution models on this type of architecture at the level of task parallelism. For this purpose, we use a highly optimized fork-join based implementation of the FMM and extend it to a data-driven implementation using a distributed task scheduling approach. This study exposes some limitations of the conventional fork-join implementation in terms of synchronization overheads. We find that these are not negligible and their elimination by the data-driven method, with a careful data locality strategy, was beneficial. Experimental evaluation of both methods on state-of-the-art multi-socket multi-core architectures showed up to 22% speed-ups of the data-driven approach compared to the original method. We demonstrate that a data-driven execution of FMM not only improves performance by avoiding global synchronization overheads but also reduces the memory-bandwidth pressure caused by memory-intensive computations. © 2013 Springer-Verlag.

  9. DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression.

    Science.gov (United States)

    Hampp, Stephanie; Kiessling, Tina; Buechle, Kerstin; Mansilla, Sabrina F; Thomale, Jürgen; Rall, Melanie; Ahn, Jinwoo; Pospiech, Helmut; Gottifredi, Vanesa; Wiesmüller, Lisa

    2016-07-26

    DNA damage tolerance facilitates the progression of replication forks that have encountered obstacles on the template strands. It involves either translesion DNA synthesis initiated by proliferating cell nuclear antigen monoubiquitination or less well-characterized fork reversal and template switch mechanisms. Herein, we characterize a novel tolerance pathway requiring the tumor suppressor p53, the translesion polymerase ι (POLι), the ubiquitin ligase Rad5-related helicase-like transcription factor (HLTF), and the SWI/SNF catalytic subunit (SNF2) translocase zinc finger ran-binding domain containing 3 (ZRANB3). This novel p53 activity is lost in the exonuclease-deficient but transcriptionally active p53(H115N) mutant. Wild-type p53, but not p53(H115N), associates with POLι in vivo. Strikingly, the concerted action of p53 and POLι decelerates nascent DNA elongation and promotes HLTF/ZRANB3-dependent recombination during unperturbed DNA replication. Particularly after cross-linker-induced replication stress, p53 and POLι also act together to promote meiotic recombination enzyme 11 (MRE11)-dependent accumulation of (phospho-)replication protein A (RPA)-coated ssDNA. These results implicate a direct role of p53 in the processing of replication forks encountering obstacles on the template strand. Our findings define an unprecedented function of p53 and POLι in the DNA damage response to endogenous or exogenous replication stress.

  10. Antecedent Rivers

    Indian Academy of Sciences (India)

    far north of the high NandaDevi (7,817 m) - Api Nampa. (7,132 m) range of the Himadri. The Sindhu flows northwestwards, the Satluj goes west, the Karnali takes the southerly course and the Tsangpo flows east. These rivers flow through their pristine channels, carved out at the very outset about 50 to 55 m.y (million years) ...

  11. Remedial design work plan for Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-10-01

    The Remedial Design Work Plan (RDWP) for Lower East Fork Poplar Creek (EFPC) Operable Unit (OU) in Oak Ridge, Tennessee. This remedial action fits into the overall Oak Ridge Reservation (ORR) cleanup strategy by addressing contaminated floodplain soil. The objective of this remedial action is to minimize the risk to human health and the environment from contaminated soil in the Lower EFPC floodplain pursuant to the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and the Federal Facility Agreement (FFA) (1992). In accordance with the FFA, a remedial investigation (RI) (DOE 1994a) and a feasibility study (DOE 1994b) were conducted to assess contamination of the Lower EFPC and propose remediation alternatives. The remedial investigation determined that the principal contaminant is mercury, which originated from releases during Y-12 Plant operations, primarily between 1953 and 1963. The recommended alternative by the feasibility study was to excavate and dispose of floodplain soils contaminated with mercury above the remedial goal option. Following the remedial investigation/feasibility study, and also in accordance with the FFA, a proposed plan was prepared to more fully describe the proposed remedy.

  12. Post construction report for Lower East Fork Poplar Creek Project, Phase 1, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-11-01

    This Phase 1 Remedial Action (RA) effort was conducted in accordance with the Record of Decision (ROD) for Lower East Fork Poplar Creek (LEFPC) as a Comprehensive Environmental Response, Compensation, and Liability Act action. The LEFPC, Phase 1 RA removed approximately 5,560 yd 3 of mercury-contaminated soils, ≥ 400 ppm, from selected portions of the National Oceanographic and Atmospheric Administration (NOAA) site LEFPC floodplain from July 8, 1996--September 14, 1996. During excavation activities, pockets of elevated radiologically contaminated soils (greater than 35 pCi/g) were located by the continuous monitoring of the excavation areas and contaminated soils with radiological monitoring instruments. Through characterization sampling it has been determined that ∼ 90 yd 3 are less than 35 pCi/g uranium contaminated and will be transported to the Y-12 Landfill V for disposal and the remaining ∼40 yd 3 do not meet the WAC for radiological constituents included in the Special Waste Permit for Landfill V. The radiologically contaminated soil will be placed in 21st Century containers for storage at the K-25 site

  13. CRITICAL CONTROL POINT IDENTIFICATION THROUGH TROPHOLOGICAL MEAT PRODUCTION CHAINFROM FIELD TO FORK

    Directory of Open Access Journals (Sweden)

    A. V. Borodin

    2017-01-01

    Full Text Available  Competitive production management is impossible without comprehensive hazard monitoring and critical parameters control at every stage of food production from raw material and auxiliary materials delivery to ready product realization, which is difficult without modern IT-support. The HACCP (Hazard Analysis and Critical Control Points approach to product safety differs from ready product testing for compliance with NaTD requirements (Normative and Technical Documentation and emphasizes the importance of the process approach to monitoring at every stage of food production. Critical control points (CCP identification is a stage, where the presence of a risk of manufacturing products that are unsafe for human health is recognized and it is possible to take action to its elimination, prevention or reduction to an acceptable level. The use of soſtware package significantly increases the enterprise HACCP system efficiency. The article describes methodological bases for IT-approach to the CCP identification in the trophological meat production chain from field to fork. The algorithmic support and soſtware for the «Decision tree», which allows detecting existing hazards, identifying risks, determining CCPs and describing them, has been developed.

  14. Risk-based decision making: The East Fork Poplar Creek case study

    Energy Technology Data Exchange (ETDEWEB)

    Moore, D.R.J.; Sample, B.E.; Suter, G.W.; Parkhurst, B.R.; Teed, R.S.

    1999-12-01

    A probabilistic risk assessment revealed that methylmercury released from the US Department of Energy (DOE) Y-12 weapons facility at Oak Ridge, Tennessee, USA, poses moderate risks to mink and kingfishers residing near the receiving waters of East Fork Poplar Creek. Polychlorinated biphenyls (PCBs) released from this facility pose severe risks to mink but little risk to kingfishers. The objective of this study was to use a risk-based decision-making approach to select remedial cleanup levels for each of these contaminants. The authors conducted Monte Carlo simulations to estimate total daily intakes of each contaminant by mink (mercury and PCBs) and kingfishers (PCBs only) for a range of exposure-reduction scenarios. The resulting exposure distributions were then integrated with their respective dose-response curves to estimate postremediation risks. The results indicated that total mercury levels in surface water would need to be reduced from current levels (mean = 0.225 {micro}g/L) to 0.03 to 0.05 {micro}g/L to reduce risks to very low levels (<5% probability of {ge}20% mortality) for both mink and kingfishers. If interested parties define acceptable risk as, for example, a 20% probability of {gt} 10% mortality, then mercury levels would need to be reduced to 0.14 {micro}g/L. The PCBs analysis indicated that reducing water-borne exposures would produce only a modest reduction in risk to mink because much of the current exposure is through terrestrial exposure pathways.

  15. An inventory of wetlands in the East Fork Poplar Creek floodplain, Anderson and Roane Counties, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-12-01

    An inventory of wetlands within the floodplain of East Fork Poplar Creek (EFPC) in Anderson and Roane Counties, Tennessee was conducted during October, 1991 through May, 1992 for the US Department of Energy (DOE) by the US Army Corps of Engineers, Nashville District. About 15 miles of EFPC channel and 500 acres of its floodplain are contaminated with mercury and other contaminants released from the Y-12 Plant on the DOE Oak Ridge Reservation. The wetland inventory will serve as baseline information for DOE`s remedial action planning and National Environmental Policy Act compliance efforts related to the contamination. In order to provide broad wetland determinations beyond which future wetland definitions are unlikely to expand, the 1989 Federal Manual for Identifying And Delineating Jurisdictional Wetlands was utilized. Using the manual`s methodology in a contaminated system under the approved health and safety plan presented some unique problems, resulting in intrusive sampling for field indicators of hydric soils being accomplished separately from observation of other criteria. Beginning with wetland areas identified on National Wetland Inventory Maps, the entire floodplain was examined for presence of wetland criteria, and 17 wetlands were identified ranging from 0.01 to 2.81 acres in size. The majority of wetlands identified were sized under 1 acre. Some of the wetlands identified were not delineated on the National Wetland Inventory Maps, and much of the wetland area delineated on the maps did not meet the criteria under the 1989 manual.

  16. Vibration-Induced Errors in MEMS Tuning Fork Gyroscopes with Imbalance.

    Science.gov (United States)

    Fang, Xiang; Dong, Linxi; Zhao, Wen-Sheng; Yan, Haixia; Teh, Kwok Siong; Wang, Gaofeng

    2018-05-29

    This paper discusses the vibration-induced error in non-ideal MEMS tuning fork gyroscopes (TFGs). Ideal TFGs which are thought to be immune to vibrations do not exist, and imbalance between two gyros of TFGs is an inevitable phenomenon. Three types of fabrication imperfections (i.e., stiffness imbalance, mass imbalance, and damping imbalance) are studied, considering different imbalance radios. We focus on the coupling types of two gyros of TFGs in both drive and sense directions, and the vibration sensitivities of four TFG designs with imbalance are simulated and compared. It is found that non-ideal TFGs with two gyros coupled both in drive and sense directions (type CC TFGs) are the most insensitive to vibrations with frequencies close to the TFG operating frequencies. However, sense-axis vibrations with in-phase resonant frequencies of a coupled gyros system result in severe error outputs to TFGs with two gyros coupled in the sense direction, which is mainly attributed to the sense capacitance nonlinearity. With increasing stiffness coupled ratio of the coupled gyros system, the sensitivity to vibrations with operating frequencies is cut down, yet sensitivity to vibrations with in-phase frequencies is amplified.

  17. Lessons learned at Lower East Fork Poplar Creek, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Burch, K.L.; Page, D.G.

    1996-01-01

    The US Department of Energy (DOE) used several innovative strategies and technologies in conducting the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA) activities for the Lower East Fork Poplar Creek (EFPC) Operable Unit (OU) in Oak Ridge, Tennessee. These innovations helped to cost-effectively characterize the 270-ha (670-acre), 23.3-km (14.5-mile) floodplain and to obtain a 400-parts per million (ppm) cleanup level for mercury in soil. Lessons learned during the project involve management, investigation, and risk assessment strategies and techniques. Management lessons learned include (a) how to handle the large OU, (b) how to effectively involve the community in decisions, and (c) how to select a remedy that incorporates the needs of many involved agencies. Investigation lessons learned include (a) how to design an effective sampling strategy for the site, (b) how to cost-effectively analyze a large number of samples, and (c) which of several treatment technologies is best-suited to the site. Risk assessment lessons learned include (a) how to determine an appropriate cleanup level for human health and the environment, (b) how to quantify uncertainty in the human health risk assessment, (c) how to reconcile different solubilities of different mercury species, and (d) how to best conduct the ecological risk assessment. Other CERCLA sites can benefit from lessons learned during this project whether still in the investigative stage or further along in the process. Applying these lessons can substantially reduce costs and make more efficient use of Superfund resources

  18. Waste Management Plan for the Lower East Fork Poplar Creek Remedial Action Project Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Landfill V, and restore the affected floodplain upon completion of remediation activities. This effort will be conducted in accordance with the Record of Decision (ROD) for LEFPC as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) action. The Waste Management Plan addresses management and disposition of all wastes generated during the remedial action for the LEFPC Project Most of the solid wastes will be considered to be sanitary or construction/demolition wastes and will be disposed of at existing Y-12 facilities for those types of waste. Some small amounts of hazardous waste are anticipated, and the possibility of low- level or mixed waste exists (greater than 35 pCi/g), although these are not expected. Liquid wastes will be generated which will be sanitary in nature and which will be capable of being disposed 0214 of at the Oak Ridge Sewage Treatment Plant.

  19. Directing the path of light-induced electron transfer at a molecular fork using vibrational excitation

    Science.gov (United States)

    Delor, Milan; Archer, Stuart A.; Keane, Theo; Meijer, Anthony J. H. M.; Sazanovich, Igor V.; Greetham, Gregory M.; Towrie, Michael; Weinstein, Julia A.

    2017-11-01

    Ultrafast electron transfer in condensed-phase molecular systems is often strongly coupled to intramolecular vibrations that can promote, suppress and direct electronic processes. Recent experiments exploring this phenomenon proved that light-induced electron transfer can be strongly modulated by vibrational excitation, suggesting a new avenue for active control over molecular function. Here, we achieve the first example of such explicit vibrational control through judicious design of a Pt(II)-acetylide charge-transfer donor-bridge-acceptor-bridge-donor 'fork' system: asymmetric 13C isotopic labelling of one of the two -C≡C- bridges makes the two parallel and otherwise identical donor→acceptor electron-transfer pathways structurally distinct, enabling independent vibrational perturbation of either. Applying an ultrafast UVpump(excitation)-IRpump(perturbation)-IRprobe(monitoring) pulse sequence, we show that the pathway that is vibrationally perturbed during UV-induced electron transfer is dramatically slowed down compared to its unperturbed counterpart. One can thus choose the dominant electron transfer pathway. The findings deliver a new opportunity for precise perturbative control of electronic energy propagation in molecular devices.

  20. Waste Management Plan for the Lower East Fork Poplar Creek Remedial Action Project Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-08-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Landfill V, and restore the affected floodplain upon completion of remediation activities. This effort will be conducted in accordance with the Record of Decision (ROD) for LEFPC as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) action. The Waste Management Plan addresses management and disposition of all wastes generated during the remedial action for the LEFPC Project Most of the solid wastes will be considered to be sanitary or construction/demolition wastes and will be disposed of at existing Y-12 facilities for those types of waste. Some small amounts of hazardous waste are anticipated, and the possibility of low- level or mixed waste exists (greater than 35 pCi/g), although these are not expected. Liquid wastes will be generated which will be sanitary in nature and which will be capable of being disposed 0214 of at the Oak Ridge Sewage Treatment Plant

  1. A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability.

    Directory of Open Access Journals (Sweden)

    2005-12-01

    Full Text Available To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately 4,700 viable yeast deletion mutants and determined the fitness of the double mutants. Fifty-six DNA2-interacting genes were identified. Clustering analysis of genomic synthetic lethality profiles of each of 43 of the DNA2-interacting genes defines a network (consisting of 322 genes and 876 interactions whose topology provides clues as to how replication proteins coordinate regulation and repair to protect genome integrity. The results also shed new light on the functions of the query gene DNA2, which, despite many years of study, remain controversial, especially its proposed role in Okazaki fragment processing and the nature of its in vivo substrates. Because of the multifunctional nature of virtually all proteins at the replication fork, the meaning of any single genetic interaction is inherently ambiguous. The multiplexing nature of the current studies, however, combined with follow-up supporting experiments, reveals most if not all of the unique pathways requiring Dna2p. These include not only Okazaki fragment processing and DNA repair but also chromatin dynamics.

  2. Salmonella and Campylobacter biofilm formation: a comparative assessment from farm to fork.

    Science.gov (United States)

    Lamas, Alexandre; Regal, Patricia; Vázquez, Beatriz; Miranda, José M; Cepeda, Alberto; Franco, Carlos M

    2018-02-09

    It takes several steps to bring food from the farm to the fork (dining table), and contamination with food-borne pathogens can occur at any point in the process. Campylobacter spp. and Salmonella spp. are the main microorganisms responsible for foodborne disease in the EU. These two pathogens are able to persist throughout the food supply chain thanks to their ability to form biofilms. Owing to the high prevalence of Salmonella and especially of Campylobacter in the food supply chain and the huge efforts of food authorities to reduce these levels, it is of great importance to fully understand their mechanisms of persistence. Diverse studies have evaluated the biofilm-forming capacity of foodborne pathogens isolated at different steps of food production. Nonetheless, the principal obstacle of these studies is to reproduce the real conditions that microorganisms encounter in the food supply chain. While there are a wide number of Salmonella biofilm studies, information on Campylobacter biofilms is still limited. A comparison between the two microorganisms could help to develop new research in the field of Campylobacter biofilms. Therefore, this review evaluates relevant work in the field of Salmonella and Campylobacter biofilms and the applicability of the data obtained from these studies to real working conditions. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

  3. Quartz-Enhanced Photoacoustic Spectroscopy Sensor with a Small-Gap Quartz Tuning Fork

    Directory of Open Access Journals (Sweden)

    Yu-Fei Ma

    2018-06-01

    Full Text Available A highly sensitive quartz-enhanced photoacoustic spectroscopy (QEPAS sensor based on a custom quartz tuning fork (QTF with a small-gap of 200 μm was demonstrated. With the help of the finite element modeling (FEM simulation software COMSOL, the change tendency of the QEPAS signal under the influence of the laser beam vertical position and the length of the micro-resonator (mR were calculated theoretically. Water vapor (H2O was selected as the target analyte. The experimental results agreed well with those of the simulation, which verified the correctness of the theoretical model. An 11-fold signal enhancement was achieved with the addition of an mR with an optimal length of 5 mm in comparison to the bare QTF. Finally, the H2O-QEPAS sensor, which was based on a small-gap QTF, achieved a minimum detection limit (MDL of 1.3 ppm, indicating an improvement of the sensor performance when compared to the standard QTF that has a gap of 300 μm.

  4. Post construction report for Lower East Fork Poplar Creek Project, Phase 1, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-11-01

    This Phase 1 Remedial Action (RA) effort was conducted in accordance with the Record of Decision (ROD) for Lower East Fork Poplar Creek (LEFPC) as a Comprehensive Environmental Response, Compensation, and Liability Act action. The LEFPC, Phase 1 RA removed approximately 5,560 yd{sup 3} of mercury-contaminated soils, {ge} 400 ppm, from selected portions of the National Oceanographic and Atmospheric Administration (NOAA) site LEFPC floodplain from July 8, 1996--September 14, 1996. During excavation activities, pockets of elevated radiologically contaminated soils (greater than 35 pCi/g) were located by the continuous monitoring of the excavation areas and contaminated soils with radiological monitoring instruments. Through characterization sampling it has been determined that {approximately} 90 yd{sup 3} are less than 35 pCi/g uranium contaminated and will be transported to the Y-12 Landfill V for disposal and the remaining {approximately}40 yd{sup 3} do not meet the WAC for radiological constituents included in the Special Waste Permit for Landfill V. The radiologically contaminated soil will be placed in 21st Century containers for storage at the K-25 site.

  5. Solar project description for Design Construction Association single family dwelling, Big Fork, Montana

    Science.gov (United States)

    1980-04-01

    A solar energy system was installed in a 2100 sq ft house located in Big Fork, Montana. The system is designed to provide solar energy for heating and domestic hot water. Solar energy is collected by flat plate collectors with a gross area of 792 square feet. The collector banks are mounted on the roof of the house and face due south at an angle of 45 deg to the horizontal optimizing solar energy collection. Solar energy is transferred from the collector array to a 1500 gallon storage tank. Water is used as the heat collection, transfer and storage medium. Freeze protection is provided by use of a drain down system. Space heating demands are met by circulating hot water from storage through baseboard units in the distribution system of the house. Auxiliary space heating is provided by an electrical heating element in the boiler. Similarly, an electrical heating element in the DHW tank provides energy for water heating. The dwelling was fully instrumented for performance evaluation since October 1977 and the data is integrated into the National Solar Data Network.

  6. Design of a Compact Tuning Fork-Shaped Notched Ultrawideband Antenna for Wireless Communication Application

    Science.gov (United States)

    Shakib, M. N.; Moghavvemi, M.; Mahadi, W. N. L.

    2014-01-01

    A new compact planar notched ultrawideband (UWB) antenna is designed for wireless communication application. The proposed antenna has a compact size of 0.182λ × 0.228λ × 0.018λ where λ is the wavelength of the lowest operating frequency. The antenna is comprised of rectangular radiating patch, ground plane, and an arc-shaped strip in between radiating patch and feed line. By introducing a new Tuning Fork-shaped notch in the radiating plane, a stopband is obtained. The antenna is tested and measured. The measured result indicated that fabricated antenna has achieved a wide bandwidth of 4.33–13.8 GHz (at −10 dB return loss) with a rejection frequency band of 5.28–6.97 GHz (WiMAX, WLAN, and C-band). The effects of the parameters of the antenna are discussed. The experiment results demonstrate that the proposed antenna can well meet the requirement for the UWB communication in spite of its compactness and small size. PMID:24723835

  7. Kinetic energy dissipation of a tuning fork immersed in superfluid helium at different frequencies of oscillations

    International Nuclear Information System (INIS)

    Gritsenko, I.A.; Klokol, K.A.; Sokolov, S.S.; Sheshin, G.A.

    2016-01-01

    An experimental study is made of the drag coefficient, which is the characteristics of energy dissipation during oscillations of the tuning forks, immersed in liquid helium. The experiments were performed in the temperature range from 0.1 to 3.5 K covering both the range of a hydrodynamic flow, and the ballistic regime of transfer of thermal excitations of superfluid helium below 0.6 K. It is found that there is the frequency dependence of the drag coefficient in the hydrodynamic limit, when the main dissipation mechanism is the viscous friction of the fluid against the walls of the oscillating body at temperatures above 0.7 K. In this case, the drag coefficient is proportional to the square root of the frequency of oscillation, and its temperature dependence in He II is determined by the respective dependence of the normal component density of the normal component and the viscosity of the fluid. At lower temperatures, the dependence of drag coefficient on the frequency is not available, and the magnitude of the dissipative losses is determined only by the temperature dependence of the density of the normal component. At the same time in the entire range of temperatures value of dissipative losses depends on the geometry of the oscillating body.

  8. Surface water of Little River basin in southeastern Oklahoma (with a section on quality of water by R. P. Orth)

    Science.gov (United States)

    Westfall, A.O.; Orth, Richard Philip

    1963-01-01

    This report summarizes basic hydrologic data of the surface water resources of Little River basin above the Oklahoma-Arkansas state line near Cerrogordo, Okla., and by analysis and interpretation, presents certain streamflow characteristics at specified points in the basin. Little River basin above the state line includes 2,269 square miles, of which about 250 square miles of the Mountain Fork River is in Arkansas. The climate is humid and the annual precipitation averages about 46 inches. Gross annual lake evaporation averages 49 inches per year. There are three reservoirs totaling 2,831,800 acre-feet of storage, either authorized or under construction in the basin. The average annual discharge at the gaging stations for the period 1930-61 is 674,900 acre-feet for Little River near Wright City; 1,273,000 acre-feet for Little River below Lukfata Creek, near Idabel; and 989,000 acre-feet for Mountain Fork River near Eagletown. The average annual discharge of Little River at the Oklahoma-Arkansas state line near Cerrogordo is 2,401,000 acre-feet. Flow-duration curves have been developed from daily records for the gaging stations. These curves show the percentage of time various rates of discharge have been equaled or exceeded. Procedures for defining the frequency of annual floods at any point in the basin are given. Low-flow frequency curves for the gaging stations defining the recurrence intervals of 7, 14 or 15, 30, 60, and 120 day mean flows have been prepared. Curves showing the relation of instantaneous discharge at specified upstream points to the daily mean discharge at two gaging stations are presented. The storage requirements for suplementing natural flows have been prepared for the gaging-station sites. Chemical analyses show that the surface water in the basin is suitable for domestic and industrial uses.

  9. U.S. Geological Survey 2013 assessment of undiscovered resources in the Bakken and Three Forks Formations of the U.S. Williston Basin Province

    Science.gov (United States)

    Gaswirth, Stephanie B.; Marra, Kristen R.

    2014-01-01

    The Upper Devonian Three Forks and Upper Devonian to Lower Mississippian Bakken Formations comprise a major United States continuous oil resource. Current exploitation of oil is from horizontal drilling and hydraulic fracturing of the Middle Member of the Bakken and upper Three Forks, with ongoing exploration of the lower Three Forks, and the Upper, Lower, and Pronghorn Members of the Bakken Formation. In 2008, the U.S. Geological Survey (USGS) estimated a mean of 3.65 billion bbl of undiscovered, technically recoverable oil resource within the Bakken Formation. The USGS recently reassessed the Bakken Formation, which included an assessment of the underlying Three Forks Formation. The Pronghorn Member of the Bakken Formation, where present, was included as part of the Three Forks assessment due to probable fluid communication between reservoirs. For the Bakken Formation, five continuous and one conventional assessment units (AUs) were defined. These AUs are modified from the 2008 AU boundaries to incorporate expanded geologic and production information. The Three Forks Formation was defined with one continuous and one conventional AU. Within the continuous AUs, optimal regions of hydrocarbon recovery, or “sweet spots,” were delineated and estimated ultimate recoveries were calculated for each continuous AU. Resulting undiscovered, technically recoverable resource estimates were 3.65 billion bbl for the five Bakken continuous oil AUs and 3.73 billion bbl for the Three Forks Continuous Oil AU, generating a total mean resource estimate of 7.38 billion bbl. The two conventional AUs are hypothetical and represent a negligible component of the total estimated resource (8 million barrels of oil).

  10. Simulation of Flow, Sediment Transport, and Sediment Mobility of the Lower Coeur d'Alene River, Idaho

    Science.gov (United States)

    Berenbrock, Charles; Tranmer, Andrew W.

    2008-01-01

    A one-dimensional sediment-transport model and a multi-dimensional hydraulic and bed shear stress model were developed to investigate the hydraulic, sediment transport, and sediment mobility characteristics of the lower Coeur d?Alene River in northern Idaho. This report documents the development and calibration of those models, as well as the results of model simulations. The one-dimensional sediment-transport model (HEC-6) was developed, calibrated, and used to simulate flow hydraulics and erosion, deposition, and transport of sediment in the lower Coeur d?Alene River. The HEC-6 modeled reach, comprised of 234 cross sections, extends from Enaville, Idaho, on the North Fork of the Coeur d?Alene River and near Pinehurst, Idaho, on the South Fork of the river to near Harrison, Idaho, on the main stem of the river. Bed-sediment samples collected by previous investigators and samples collected for this study in 2005 were used in the model. Sediment discharge curves from a previous study were updated using suspended-sediment samples collected at three sites since April 2000. The HEC-6 was calibrated using river discharge and water-surface elevations measured at five U.S. Geological Survey gaging stations. The calibrated HEC-6 model allowed simulation of management alternatives to assess erosion and deposition from proposed dredging of contaminated streambed sediments in the Dudley reach. Four management alternatives were simulated with HEC-6. Before the start of simulation for these alternatives, seven cross sections in the reach near Dudley, Idaho, were deepened 20 feet?removing about 296,000 cubic yards of sediments?to simulate dredging. Management alternative 1 simulated stage-discharge conditions from 2000, and alternative 2 simulated conditions from 1997. Results from alternatives 1 and 2 indicated that about 6,500 and 12,300 cubic yards, respectively, were deposited in the dredged reach. These figures represent 2 and 4 percent, respectively, of the total volume of

  11. River Corridor Easements

    Data.gov (United States)

    Vermont Center for Geographic Information — A River Corridor Easement (RCE) is an area of conserved land adjacent to a river or stream that was conserved to permanently protect the lateral area the river needs...

  12. Assessment of Salmonids and Their Habitat Conditions in the Walla Walla River Basin within Washington, Annual Report 2002-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Mendel, Glen; Trump, Jeremy; Gembala, Mike

    2003-09-01

    trutta) had low densities, and limited distribution throughout the basin. A large return of adult spring chinook to the Touchet River drainage in 2001 produced higher densities of juvenile chinook in 2002 than have been seen in recent years, especially in the Wolf Fork. The adult return in 2002 was substantially less than what was seen in 2001. Due to poor water conditions and trouble getting personnel hired, spawning surveys were limited in 2002. Surveyors found only one redd in four Walla Walla River tributaries (Cottonwood Ck., East Little Walla Walla, West Little Walla Walla, and Mill Ck.), and 59 redds in Touchet River tributaries (10 in the North Fork Touchet, 30 in the South Fork Touchet, and 19 in the Wolf Fork). Bull trout spawning surveys in the upper Touchet River tributaries found a total of 125 redds and 150 live fish (92 redds and 75 fish in the Wolf Fork, 2 redds and 1 fish in the Burnt Fork, 0 redds and 1 fish in the South Fork Touchet, 29 redds and 71 fish in the North Fork Touchet, and 2 redds and 2 fish in Lewis Ck.). A preliminary steelhead genetics analysis was completed as part of this project. Results indicate differences between naturally produced steelhead and those produced in the hatchery. There were also apparent genetic differences among the naturally produced fish from different areas of the basin. Detailed results are reported in Bumgarner et al. 2003. Recommendations for assessment activities in 2003 included: (1) continue to monitor the Walla Walla River (focusing from the stateline to McDonald Rd.), the Mill Ck system, and the Little Walla Walla System. (2) reevaluate Whiskey Ck. for abundance and distribution of salmonids, and Lewis Ck. for bull trout density and distribution. (3) select or develop a habitat survey protocol and begin to conduct habitat inventory and assessment surveys. (4) summarize bull trout data for Mill Ck, South Fork Touchet, and Lewis Ck. (5) begin to evaluate temperature and flow data to assess if the habitat

  13. River Diversions and Shoaling

    National Research Council Canada - National Science Library

    Letter, Jr., Joseph V; Pinkard, Jr., C. F; Raphelt, Nolan K

    2008-01-01

    This Coastal and Hydraulics Engineering Technical Note describes the current knowledge of the potential impacts of river diversions on channel morphology, especially induced sedimentation in the river channel...

  14. Streamflow statistics for development of water rights claims for the Jarbidge Wild and Scenic River, Owyhee Canyonlands Wilderness, Idaho, 2013-14: a supplement to Scientific Investigations Report 2013-5212

    Science.gov (United States)

    Wood, Molly S.

    2014-01-01

    The U.S. Geological Survey (USGS), in cooperation with the Bureau of Land Management (BLM), estimated streamflow statistics for stream segments designated “Wild,” “Scenic,” or “Recreational” under the National Wild and Scenic Rivers System in the Owyhee Canyonlands Wilderness in southwestern Idaho. The streamflow statistics were used by the BLM to develop and file a draft, federal reserved water right claim to protect federally designated “outstanding remarkable values” in the Jarbidge River. The BLM determined that the daily mean streamflow equaled or exceeded 20, 50, and 80 percent of the time during bimonthly periods (two periods per month) and the bankfull (66.7-percent annual exceedance probability) streamflow are important thresholds for maintaining outstanding remarkable values. Although streamflow statistics for the Jarbidge River below Jarbidge, Nevada (USGS 13162225) were published previously in 2013 and used for the draft water right claim, the BLM and USGS have since recognized the need to refine streamflow statistics given the approximate 40 river mile distance and intervening tributaries between the original point of estimation (USGS 13162225) and at the mouth of the Jarbidge River, which is the downstream end of the Wild and Scenic River segment. A drainage-area-ratio method was used in 2013 to estimate bimonthly exceedance probability streamflow statistics at the mouth of the Jarbidge River based on available streamgage data on the Jarbidge and East Fork Jarbidge Rivers. The resulting bimonthly streamflow statistics were further adjusted using a scaling factor calculated from a water balance on streamflow statistics calculated for the Bruneau and East Fork Bruneau Rivers and Sheep Creek. The final, adjusted bimonthly exceedance probability and bankfull streamflow statistics compared well with available verification datasets (including discrete streamflow measurements made at the mouth of the Jarbidge River) and are considered the

  15. Long-Term Movement and Estimated Age of a Paddlefish (Polyodon spathula) in the Arkansas River Basin of Oklahoma

    Science.gov (United States)

    Long, James M.

    2018-01-01

    We report the age and distance moved for an individual paddlefish (Polyodon spathula) that was tagged March 1998 in the Cimarron River Arm of Keystone Lake, Oklahoma, and snagged by an angler in April 2016 downstream of Eufaula Dam, Oklahoma. The fish was part of a cohort spawned in 1995. At the time of initial capture, the fish measured 795 mm eye–fork length, was estimated to be 3 y old, and 18 y had elapsed before its recapture by an angler in 2016, indicating this fish was 21 y old at recapture. Although paddlefish as old as 27 have been estimated in the Grand River basin of Oklahoma, this is the oldest fish known in the Arkansas River basin of Oklahoma. At the place of its recapture, this fish would have traveled approximately 235 km, passing downstream through three dams before moving upstream to Eufaula Dam.

  16. Human and ecological remediation goals for soil mercury at East Fork Poplar Creek, Oak Ridge, TN

    International Nuclear Information System (INIS)

    Zafran, F.A.; Cornaby, B.W.; Hadden, C.T.

    1995-01-01

    Mercury, used in the past production of enriched lithium by the Department of Energy, is the principal chemical of concern in the 14-mile floodplain of East Fork Poplar Creek (EFPC). SAIC has developed risk-based remediation goal options (RGOS) for mercury in EFPC soils to protect the most sensitive human receptors. The existing chronic oral RfD for mercury is based on exposure of laboratory species to mercuric chloride. However, speciation and leaching/availability studies (conducted by EPA EMSL and Oak Ridge National Laboratory) indicated less soluble and less toxic mercury species, principally mercuric sulfide, with measurable quantities of metallic mercury also present, predominate in EFPC floodplain soils. SAIC derived human health RGOs using deterministic and probabilistic methods and incorporated the probability density function for bioavailability of mercury species from leaching/availability data generated by ORNL. Monte Carlo simulation was used in uncertainty analysis and supported the derivation of a protective, but realistic risk-based remediation goal of 400 mg mercury/kg soil. For ecological risk assessment, RGOs were based on risks through food chains from contaminants in soil. The authors describe a terrestrial food-chain model of contaminant transfer to primary producers, first-order consumers, mid-level predators, and top-level predators. The model uses published toxicity data, site-specific contaminant concentrations, and bioaccumulation factors calculated from measured body burdens of floodplain organisms to compute RGOs for various combinations of exposure parameters. Model calculations show that under reasonably conservative conditions, mid-level predators have the highest exposures relative to dietary limits and, therefore, require the lowest soil-mercury RGOs. Mercury concentrations of ∼500 mg/kg are protective of the receptor populations exposed through food chains at this site

  17. The Poultry-Associated Microbiome: Network Analysis and Farm-to-Fork Characterizations

    Science.gov (United States)

    Oakley, Brian B.; Morales, Cesar A.; Line, J.; Berrang, Mark E.; Meinersmann, Richard J.; Tillman, Glenn E.; Wise, Mark G.; Siragusa, Gregory R.; Hiett, Kelli L.; Seal, Bruce S.

    2013-01-01

    Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS), quantitative-PCR assays, and network analysis to profile the poultry-associated microbiome and important pathogens at various stages of commercial poultry production from the farm to the consumer. Analysis of longitudinal data following two flocks from the farm through processing showed a core microbiome containing multiple sequence types most closely related to genera known to be pathogenic for animals and/or humans, including Campylobacter, Clostridium, and Shigella. After the final stage of commercial poultry processing, taxonomic richness was ca. 2–4 times lower than the richness of fecal samples from the same flocks and Campylobacter abundance was significantly reduced. Interestingly, however, carcasses sampled at 48 hr after processing harboured the greatest proportion of unique taxa (those not encountered in other samples), significantly more than expected by chance. Among these were anaerobes such as Prevotella, Veillonella, Leptrotrichia, and multiple Campylobacter sequence types. Retail products were dominated by Pseudomonas, but also contained 27 other genera, most of which were potentially metabolically active and encountered in on-farm samples. Network analysis was focused on the foodborne pathogen Campylobacter and revealed a majority of sequence types with no significant interactions with other taxa, perhaps explaining the limited efficacy of previous attempts at competitive exclusion of Campylobacter. These data represent the first use of HTS to characterize the poultry microbiome across a series of farm-to-fork samples and demonstrate the utility of HTS in monitoring the food supply chain and identifying sources of potential zoonoses and interactions among taxa in complex communities. PMID:23468931

  18. Quartz tuning fork based sensor for detection of volatile organic compounds: towards breath analysis

    Science.gov (United States)

    Sampson, Abraham; Panchal, Suresh; Phadke, Apoorva; Kashyap, A.; Suman, Jilma; Unnikrishnan, G.; Datar, Suwarna

    2018-04-01

    Several volatile organic compounds (VOCs) are present in the exhaled human breath whose concentration can vary depending on the physiological changes occurring within a human being. These changes in the concentration or the occurrence of a particular VOC can be used as signature of a particular disease in a person. In the present work, a sensor has been developed to detect VOCs such as 1,4-dimethoxy-2,3-butanediol (BD), and cyclohexanone (CH), acetone, methanol and ethanol. Except for BD and CH, the rest of the VOCs are present in a healthy person in ppm levels. CH and BD have been reported to be present in the exhaled human breath of breast cancer patients in ppm levels and can be used to distinguish between a healthy person and a person with breast cancer. The selectivity of the sensor towards these two compounds in the presence of other VOCs commonly present in human breath like acetone, ethanol and methanol has been studied. The sensor has been developed using modified Quartz Tuning Forks (QTFs) with the intent of developing an array of such sensors identifying different VOCs present in a healthy human’s breath. Two differently modified QTFs have been used to detect 1 ppm of 1,4-dimethoxy-2,3-butanediol and 20 ppm of cyclohexanone. Linear Discriminants Analysis (LDA) has been used to classify seven different VOCs. For this purpose, features extracted from sensor responses -shift in resonant frequency, response time and recovery time of the sensors- have been used as features in the model. Differently modified array of QTFs along with the use of LDA can be a useful pathway towards development of a QTF based sensor array for human breath analysis.

  19. The poultry-associated microbiome: network analysis and farm-to-fork characterizations.

    Directory of Open Access Journals (Sweden)

    Brian B Oakley

    Full Text Available Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS, quantitative-PCR assays, and network analysis to profile the poultry-associated microbiome and important pathogens at various stages of commercial poultry production from the farm to the consumer. Analysis of longitudinal data following two flocks from the farm through processing showed a core microbiome containing multiple sequence types most closely related to genera known to be pathogenic for animals and/or humans, including Campylobacter, Clostridium, and Shigella. After the final stage of commercial poultry processing, taxonomic richness was ca. 2-4 times lower than the richness of fecal samples from the same flocks and Campylobacter abundance was significantly reduced. Interestingly, however, carcasses sampled at 48 hr after processing harboured the greatest proportion of unique taxa (those not encountered in other samples, significantly more than expected by chance. Among these were anaerobes such as Prevotella, Veillonella, Leptrotrichia, and multiple Campylobacter sequence types. Retail products were dominated by Pseudomonas, but also contained 27 other genera, most of which were potentially metabolically active and encountered in on-farm samples. Network analysis was focused on the foodborne pathogen Campylobacter and revealed a majority of sequence types with no significant interactions with other taxa, perhaps explaining the limited efficacy of previous attempts at competitive exclusion of Campylobacter. These data represent the first use of HTS to characterize the poultry microbiome across a series of farm-to-fork samples and demonstrate the utility of HTS in monitoring the food supply chain and identifying sources of potential zoonoses and interactions among taxa in complex communities.

  20. Remedial design work plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-02-01

    The Remedial Design Work Plan (RDWP) for the Lower East Fork Poplar Creek (EFPC) Operable Unit (OU) in Oak Ridge, Tennessee, has been prepared. The remedial investigation determined that the principal contaminant is mercury, which originated from releases during Y-12 Plant operations, primarily between 1953 and 1963. The recommended alternative, as stated in the Record of Decision (ROD) was to excavate and dispose of floodplain soils contaminated with mercury above the remedial goal option. Thereafter, a public hearing was held to review the proposed plan. Comments were incorporated. The revised selected remedy, per the ROD is to excavate and dispose of floodplain soils contaminated above the remediation goal of 400 parts per million mercury. The approved ROD with this goal will be the basis for remedial design (RD). The RD work plan (RDWP) is composed of six chapters. An introductory chapter describes the purpose and scope of the RDWP, the selected remedy as identified by the ROD; the roles and responsibilities of the RD team members, and the site background information, including site history, contaminants of concern, and site characteristics. Chapter 2 contains the design objectives, RD approach, regulatory considerations during RD, and the design criteria with assumptions. Chapter 3 presents the RD planning process to prepare this RDWP, as well as secondary RD support plans. Chapter 4 describes the scope of the RD activities in more detail and identifies what will be included in the design package. Chapter 5 presents the schedule for performance of the RD activities, identifying key RD milestones. Specific documents used in the preparation of this document are referenced in Chapter 6

  1. Bull Trout Population Assessment in the Columbia River Gorge : Annual Report 2000.

    Energy Technology Data Exchange (ETDEWEB)

    Byrne, Jim; McPeak, Ron

    2001-02-01

    We summarized existing knowledge regarding the known distribution of bull trout (Salvelinus confluentus) across four sub-basins in the Columbia River Gorge in Washington. The Wind River, Little White Salmon River, White Salmon River, and the Klickitat River sub-basins were analyzed. Cold water is essential to the survival, spawning, and rearing of bull trout. We analyzed existing temperature data, installed Onset temperature loggers in the areas of the four sub-basins where data was not available, and determined that mean daily water temperatures were <15 C and appropriate for spawning and rearing of bull trout. We snorkel surveyed more than 74 km (46.25 mi.) of rivers and streams in the four sub-basins (13.8 km at night and 60.2 km during the day) and found that night snorkeling was superior to day snorkeling for locating bull trout. Surveys incorporated the Draft Interim Protocol for Determining Bull Trout Presence (Peterson et al. In Press). However, due to access and safety issues, we were unable to randomly select sample sites nor use block nets as recommended. Additionally, we also implemented the Bull Trout/Dolly Varden sampling methodology described in Bonar et al. (1997). No bull trout were found in the Wind River, Little White Salmon, or White Salmon River sub-basins. We found bull trout in the West Fork Klickitat drainage of the Klickitat River Sub-basin. Bull trout averaged 6.7 fish/100m{sup 2} in Trappers Creek, 2.6 fish/100m{sup 2} on Clearwater Creek, and 0.4 fish/100m{sup 2} in Little Muddy Creek. Bull trout was the only species of salmonid encountered in Trappers Creek and dominated in Clearwater Creek. Little Muddy Creek was the only creek where bull trout and introduced brook trout occurred together. We found bull trout only at night and typically in low flow regimes. A single fish, believed to be a bull trout x brook trout hybrid, was observed in the Little Muddy Creek. Additional surveys are needed in the West Fork Klickitat and mainstem

  2. Bull trout population assessment in the Columbia River Gorge/annual report fy2000; ANNUAL

    International Nuclear Information System (INIS)

    Byrne, Jim; McPeak, Ron

    2001-01-01

    We summarized existing knowledge regarding the known distribution of bull trout (Salvelinus confluentus) across four sub-basins in the Columbia River Gorge in Washington. The Wind River, Little White Salmon River, White Salmon River, and the Klickitat River sub-basins were analyzed. Cold water is essential to the survival, spawning, and rearing of bull trout. We analyzed existing temperature data, installed Onset temperature loggers in the areas of the four sub-basins where data was not available, and determined that mean daily water temperatures were and lt;15 C and appropriate for spawning and rearing of bull trout. We snorkel surveyed more than 74 km (46.25 mi.) of rivers and streams in the four sub-basins (13.8 km at night and 60.2 km during the day) and found that night snorkeling was superior to day snorkeling for locating bull trout. Surveys incorporated the Draft Interim Protocol for Determining Bull Trout Presence (Peterson et al. In Press). However, due to access and safety issues, we were unable to randomly select sample sites nor use block nets as recommended. Additionally, we also implemented the Bull Trout/Dolly Varden sampling methodology described in Bonar et al. (1997). No bull trout were found in the Wind River, Little White Salmon, or White Salmon River sub-basins. We found bull trout in the West Fork Klickitat drainage of the Klickitat River Sub-basin. Bull trout averaged 6.7 fish/100m(sup 2) in Trappers Creek, 2.6 fish/100m(sup 2) on Clearwater Creek, and 0.4 fish/100m(sup 2) in Little Muddy Creek. Bull trout was the only species of salmonid encountered in Trappers Creek and dominated in Clearwater Creek. Little Muddy Creek was the only creek where bull trout and introduced brook trout occurred together. We found bull trout only at night and typically in low flow regimes. A single fish, believed to be a bull trout x brook trout hybrid, was observed in the Little Muddy Creek. Additional surveys are needed in the West Fork Klickitat and mainstem

  3. A ruthenium polypyridyl intercalator stalls DNA replication forks, radiosensitizes human cancer cells and is enhanced by Chk1 inhibition

    Science.gov (United States)

    Gill, Martin R.; Harun, Siti Norain; Halder, Swagata; Boghozian, Ramon A.; Ramadan, Kristijan; Ahmad, Haslina; Vallis, Katherine A.

    2016-08-01

    Ruthenium(II) polypyridyl complexes can intercalate DNA with high affinity and prevent cell proliferation; however, the direct impact of ruthenium-based intercalation on cellular DNA replication remains unknown. Here we show the multi-intercalator [Ru(dppz)2(PIP)]2+ (dppz = dipyridophenazine, PIP = 2-(phenyl)imidazo[4,5-f][1,10]phenanthroline) immediately stalls replication fork progression in HeLa human cervical cancer cells. In response to this replication blockade, the DNA damage response (DDR) cell signalling network is activated, with checkpoint kinase 1 (Chk1) activation indicating prolonged replication-associated DNA damage, and cell proliferation is inhibited by G1-S cell-cycle arrest. Co-incubation with a Chk1 inhibitor achieves synergistic apoptosis in cancer cells, with a significant increase in phospho(Ser139) histone H2AX (γ-H2AX) levels and foci indicating increased conversion of stalled replication forks to double-strand breaks (DSBs). Normal human epithelial cells remain unaffected by this concurrent treatment. Furthermore, pre-treatment of HeLa cells with [Ru(dppz)2(PIP)]2+ before external beam ionising radiation results in a supra-additive decrease in cell survival accompanied by increased γ-H2AX expression, indicating the compound functions as a radiosensitizer. Together, these results indicate ruthenium-based intercalation can block replication fork progression and demonstrate how these DNA-binding agents may be combined with DDR inhibitors or ionising radiation to achieve more efficient cancer cell killing.

  4. The effect of real-time vibrotactile feedback delivered through an augmented fork on eating rate, satiation, and food intake.

    Science.gov (United States)

    Hermans, Roel C J; Hermsen, Sander; Robinson, Eric; Higgs, Suzanne; Mars, Monica; Frost, Jeana H

    2017-06-01

    Eating rate is a basic determinant of appetite regulation, as people who eat more slowly feel sated earlier and eat less. Without assistance, eating rate is difficult to modify due to its automatic nature. In the current study, participants used an augmented fork that aimed to decelerate their rate of eating. A total of 114 participants were randomly assigned to the Feedback Condition (FC), in which they received vibrotactile feedback from their fork when eating too fast (i.e., taking more than one bite per 10 s), or a Non-Feedback Condition (NFC). Participants in the FC took fewer bites per minute than did those in the NFC. Participants in the FC also had a higher success ratio, indicating that they had significantly more bites outside the designated time interval of 10 s than did participants in the NFC. A slower eating rate, however, did not lead to a significant reduction in the amount of food consumed or level of satiation. These findings indicate that real-time vibrotactile feedback delivered through an augmented fork is capable of reducing eating rate, but there is no evidence from this study that this reduction in eating rate is translated into an increase in satiation or reduction in food consumption. Overall, this study shows that real-time vibrotactile feedback may be a viable tool in interventions that aim to reduce eating rate. The long-term effectiveness of this form of feedback on satiation and food consumption, however, awaits further investigation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Scanning thermal microscopy based on a quartz tuning fork and a micro-thermocouple in active mode (2ω method)

    International Nuclear Information System (INIS)

    Bontempi, Alexia; Nguyen, Tran Phong; Salut, Roland; Thiery, Laurent; Teyssieux, Damien; Vairac, Pascal

    2016-01-01

    A novel probe for scanning thermal microscope using a micro-thermocouple probe placed on a Quartz Tuning Fork (QTF) is presented. Instead of using an external deflection with a cantilever beam for contact detection, an original combination of piezoelectric resonator and thermal probe is employed. Due to a non-contact photothermal excitation principle, the high quality factor of the QTF allows the probe-to-surface contact detection. Topographic and thermal scanning images obtained on a specific sample points out the interest of our system as an alternative to cantilevered resistive probe systems which are the most spread.

  6. Scanning thermal microscopy based on a quartz tuning fork and a micro-thermocouple in active mode (2ω method).

    Science.gov (United States)

    Bontempi, Alexia; Nguyen, Tran Phong; Salut, Roland; Thiery, Laurent; Teyssieux, Damien; Vairac, Pascal

    2016-06-01

    A novel probe for scanning thermal microscope using a micro-thermocouple probe placed on a Quartz Tuning Fork (QTF) is presented. Instead of using an external deflection with a cantilever beam for contact detection, an original combination of piezoelectric resonator and thermal probe is employed. Due to a non-contact photothermal excitation principle, the high quality factor of the QTF allows the probe-to-surface contact detection. Topographic and thermal scanning images obtained on a specific sample points out the interest of our system as an alternative to cantilevered resistive probe systems which are the most spread.

  7. Scanning thermal microscopy based on a quartz tuning fork and a micro-thermocouple in active mode (2ω method)

    Energy Technology Data Exchange (ETDEWEB)

    Bontempi, Alexia; Nguyen, Tran Phong; Salut, Roland; Thiery, Laurent; Teyssieux, Damien; Vairac, Pascal [FEMTO-ST Institute UMR 6174, Université de Franche-Comté, CNRS, ENSMM, UTBM, 15B Avenue des Montboucons, F-25030 Besançon (France)

    2016-06-15

    A novel probe for scanning thermal microscope using a micro-thermocouple probe placed on a Quartz Tuning Fork (QTF) is presented. Instead of using an external deflection with a cantilever beam for contact detection, an original combination of piezoelectric resonator and thermal probe is employed. Due to a non-contact photothermal excitation principle, the high quality factor of the QTF allows the probe-to-surface contact detection. Topographic and thermal scanning images obtained on a specific sample points out the interest of our system as an alternative to cantilevered resistive probe systems which are the most spread.

  8. Environmental compliance plan for the Lower East Fork Poplar Creek Remedial Action Project at Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-07-01

    Remedial action for Lower East Fork Poplar Creek, as defined by the Record of Decision, requires that soil contaminated with >400 ppM mercury be excavated and disposed. Based on the remediation goal, soil will be excavated from areas located at the NOAA site and the Bruner site and disposed at the Industrial Landfill V at the Y-12 Plant. Objective is to minimize the risk to human health and the environment from contaminated soil in the lower EFPC floodplain pursuant to CERCLA and the Federal Facility Agreement (DOE 1992)

  9. Characterization and 3D reservoir modelling of fluvial sandstones of the Williams Fork Formation, Rulison Field, Piceance Basin, Colorado, USA

    International Nuclear Information System (INIS)

    Pranter, Matthew J; Vargas, Marielis F; Davis, Thomas L

    2008-01-01

    This study describes the stratigraphic characteristics and distribution of fluvial deposits of the Upper Cretaceous Williams Fork Formation in a portion of Rulison Field and addresses 3D geologic modelling of reservoir sand bodies and their associated connectivity. Fluvial deposits include isolated and stacked point-bar deposits, crevasse splays and overbank (floodplain) mudrock. Within the Williams Fork Formation, the distribution and connectivity of fluvial sandstones significantly impact reservoir productivity and ultimate recovery. The reservoir sandstones are primarily fluvial point-bar deposits interbedded with shales and coals. Because of the lenticular geometry and limited lateral extent of the reservoir sandstones (common apparent widths of ∼500–1000 ft; ∼150–300 m), relatively high well densities (e.g. 10 acre (660 ft; 200 m) spacing) are often required to deplete the reservoir. Heterogeneity of these fluvial deposits includes larger scale stratigraphic variability associated with vertical stacking patterns and structural heterogeneities associated with faults that exhibit lateral and reverse offsets. The discontinuous character of the fluvial sandstones and lack of distinct marker beds in the middle and upper parts of the Williams Fork Formation make correlation between wells tenuous, even at a 10 acre well spacing. Some intervals of thicker and amalgamated sandstones within the middle and upper Williams Fork Formation can be correlated across greater distances. To aid correlation and for 3D reservoir modelling, vertical lithology proportion curves were used to estimate stratigraphic trends and define the stratigraphic zonation within the reservoir interval. Object-based and indicator-based modelling methods have been applied to the same data and results from the models were compared. Results from the 3D modelling indicate that sandstone connectivity increases with net-to-gross ratio and, at lower net-to-gross ratios (<30%), differences exist in

  10. Baseline and Postremediation Monitoring Program Plan for the Lower East Fork Poplar Creek operable unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This report was prepared in accordance with CERCLA requirements to present the plan for baseline and postremediation monitoring as part of the selected remedy. It provides the Environmental Restoration Program with information about the requirements to monitor for soil and terrestrial biota in the Lower East Fork Poplar Creek (LEFPC) floodplain; sediment, surface water, and aquatic biota in LEFPC; wetland restoration in the LEFPC floodplain; and human use of shallow groundwater wells in the LEFPC floodplain for drinking water. This document describes the monitoring program that will ensure that actions taken under Phases I and II of the LEFPC remedial action are protective of human health and the environment

  11. Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonaradian Age) Reservoirs, West Texas and New Mexico

    International Nuclear Information System (INIS)

    Lucia, F.J.; Ruppel, S.C.

    2001-01-01

    Characterization of cycle and facies architecture on lower Clear Fork and lowermost upper Clear Fork equivalent outcrops in Apache Canyon of Sierra Diablo was complete. The focus of detailed study in Apache Canyon has been the upper Clear Fork section because this interval contains the productive interval in South Wasson field, the preliminary subsurface study area. Parts of three high-frequency sequences (HFS), each 60 to 100 ft thick, are present on the south wall of Apache Canyon. HFS's display an upper-deepening or backstepping pattern associated with longer-term sea level rise. Each HFS is composed of upward-shallowing cycles whose thickness, facies composition, and continuity vary within and between HFS's

  12. Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonaradian Age) Reservoirs, West Texas and New Mexico, Semi-Annual; SEMIANNUAL

    International Nuclear Information System (INIS)

    Ruppel, Stephen C.; Jennings, James W.; Laubach, Stephen E.

    2001-01-01

    Outcrop studies include stratigraphic and petrophysical analysis. Analysis of the detailed sequence- and cycle-scale architecture of the Clear Fork reservoir-equivalent outcrops in Apache Canyon is nearly complete. This work reveals two high-frequency transgressive-regressive sequences (HFS) in the lower Clear Fork composite depositional sequence and three HFS in the basal middle Clear Fork composite depositional sequence. A 1,800-ft transect of 1-inch-diameter samples was collected from one cycle at the Apache Canyon outcrop. The transect was sampled with 5-ft spacing, but there were some gaps due to cover and cliff, resulting in 181 samples. Permeability, porosity, and grain density were measured, and the spatial statistics are being analyzed geostatistically

  13. Columbia River: Terminal fisheries research project. 1994 Annual report

    Energy Technology Data Exchange (ETDEWEB)

    Hirose, P.; Miller, M.; Hill, J.

    1996-12-01

    Columbia River terminal fisheries have been conducted in Youngs Bay, Oregon, since the early 1960`s targeting coho salmon produced at the state facility on the North Fork Klaskanine River. In 1977 the Clatsop County Economic Development Council`s (CEDC) Fisheries Project began augmenting the Oregon Department of Fish and Wildlife production efforts. Together ODFW and CEDC smolt releases totaled 5,060,000 coho and 411,300 spring chinook in 1993 with most of the releases from the net pen acclimation program. During 1980-82 fall commercial terminal fisheries were conducted adjacent to the mouth of Big Creek in Oregon. All past terminal fisheries were successful in harvesting surplus hatchery fish with minimal impact on nonlocal weak stocks. In 1993 the Northwest Power Planning Council recommended in its` Strategy for Salmon that terminal fishing sites be identified and developed. The Council called on the Bonneville Power Administration to fund a 10-year study to investigate the feasibility of creating and expanding terminal known stock fisheries in the Columbia River Basin. The findings of the initial year of the study are included in this report. The geographic area considered for study extends from Bonneville Dam to the river mouth. The initial year`s work is the beginning of a 2-year research stage to investigate potential sites, salmon stocks, and methodologies; a second 3-year stage will focus on expansion in Youngs Bay and experimental releases into sites with greatest potential; and a final 5-year phase establishing programs at full capacity at all acceptable sites. After ranking all possible sites using five harvest and five rearing criteria, four sites in Oregon (Tongue Point, Blind Slough, Clifton Channel and Wallace Slough) and three in Washington (Deep River, Steamboat Slough and Cathlamet Channel) were chosen for study.

  14. Geomorphic and vegetation processes of the Willamette River floodplain, Oregon: current understanding and unanswered science questions

    Science.gov (United States)

    Wallick, J. Rose; Jones, Krista L.; O'Connor, Jim E.; Keith, Mackenzie K.; Hulse, David; Gregory, Stanley V.

    2013-01-01

    This report summarizes the current understanding of floodplain processes and landforms for the Willamette River and its major tributaries. The area of focus encompasses the main stem Willamette River above Newberg and the portions of the Coast Fork Willamette, Middle Fork Willamette, McKenzie, and North, South and main stem Santiam Rivers downstream of U.S. Army Corps of Engineers dams. These reaches constitute a large portion of the alluvial, salmon-bearing rivers in the Willamette Basin. The geomorphic, or historical, floodplain of these rivers has two zones - the active channel where coarse sediment is mobilized and transported during annual flooding and overbank areas where fine sediment is deposited during higher magnitude floods. Historically, characteristics of the rivers and geomorphic floodplain (including longitudinal patterns in channel complexity and the abundance of side channels, islands and gravel bars) were controlled by the interactions between floods and the transport of coarse sediment and large wood. Local channel responses to these interactions were then shaped by geologic features like bedrock outcrops and variations in channel slope. Over the last 150 years, floods and the transport of coarse sediment and large wood have been substantially reduced in the basin. With dam regulation, nearly all peak flows are now confined to the main channels. Large floods (greater than 10-year recurrence interval prior to basinwide flow regulation) have been largely eliminated. Also, the magnitude and frequency of small floods (events that formerly recurred every 2–10 years) have decreased substantially. The large dams trap an estimated 50–60 percent of bed-material sediment—the building block of active channel habitats—that historically entered the Willamette River. They also trap more than 80 percent of the estimated bed material in the lower South Santiam River and Middle and Coast Forks of the Willamette River. Downstream, revetments further

  15. Development and Validation of a Simple Analytical Model of the Proton Exchange Membrane Fuel Cell (Pemfc) in a Fork-Lift Truck Power System

    DEFF Research Database (Denmark)

    Hosseinzadeh, Elham; Rokni, Masoud

    2013-01-01

    In this study, a general proton exchange membrane fuel cell (PEMFC) model has been developed in order to investigate the balance of plant of a fork-lift truck thermodynamically. The model takes into account the effects of pressure losses, water crossovers, humidity aspects, and voltage overpotent......In this study, a general proton exchange membrane fuel cell (PEMFC) model has been developed in order to investigate the balance of plant of a fork-lift truck thermodynamically. The model takes into account the effects of pressure losses, water crossovers, humidity aspects, and voltage...

  16. New approach to Fork measurements data analysis by RADAR-CRISP and ORIGEN integration

    International Nuclear Information System (INIS)

    Vaccaro, S.; Svedkauskaite, J.; Smejkal, A.; Schwalbach, P.; De Baere, P.; Hu, J.; Gauld, I.C.

    2013-06-01

    Currently, in the EU, activities related to interim storage of spent fuel are constantly increasing. This is particularly true in Finland and Sweden, where final geological repository sites are planned to be operational in 2023 and 2026 respectively, but also in several other countries where fuel is moved from wet ponds to dry storage (Germany, Belgium, Spain, Czech, Bulgaria, etc). The required verification activities present a considerable challenge to the EURATOM Safeguards authority.. Both EURATOM and IAEA safeguards need to know what is in the storage casks and keep continuity of knowledge of the spent fuel. A frequently-used tool for the verification of the nuclear material during loading is the 'Fork' detectors for gross gamma and neutron counting. The IT applications RADAR (Remote Acquisition of Data and Review) and CRISP (Central RADAR Inspection Support Package), developed by EURATOM, are used to acquire safeguards measurement data and to analyze them in order to verify the declarations of the nuclear plant operators. Under the framework of the U.S. DOE-EURATOM Agreement on nuclear safeguards and security, a module for automated analysis of spent fuel measurement data using the ORIGEN (Oak Ridge Isotope Generation) code, part of the SCALE nuclear systems modeling and simulation package, has been integrated into CRISP. Measurement data are collected in an unattended mode by RADAR and then processed by CRISP, which outputs, for each fuel assembly, the measured gamma and neutron count rates. Simultaneously, ORIGEN performs burn-up calculations based on operator declarations previously entered into CRISP and calculates the expected neutron and gamma count rates for each assembly. These calculations also used response functions, developed using Monte Carlo modeling, to account for the detection probabilities of both neutron and photon particles that originated in each fuel pin. Finally, CRISP correlates and compares the expected (calculated) gamma

  17. The role of psychological determinants and demographic factors in consumer demand for farm-to-fork traceability systems.

    Science.gov (United States)

    Myae, Aye Chan; Goddard, Ellen; Aubeeluck, Ashwina

    2011-01-01

    Traceability systems are an important tool (1) for tracking, monitoring, and managing product flows through the supply chain for better efficiency and profitability of suppliers, and (2) to improve consumer confidence in the face of serious food safety incidents. After the global bovine spongiform encephalopathy (BSE) crisis affected producers, consumers, trade, and the health status of animals and humans, new systems to help confirm the status of cattle products along the supply chain from farm to fork were implemented in many countries (Trautman et al. 2008 ). In this study, people's overall food safety beliefs are explored with the main objective of measuring the link between their food safety beliefs and their attitudes toward traceability. A comparison is made among English-speaking Canadians, French-speaking Canadians, and Japanese consumers. In the study, an Internet-based survey was used to collect data from nationally representative samples of the population in Canada-English (1275), Canada-French (343), and Japanese (1940) in the summer of 2009. Respondents' interests in traceability systems are clearly linked to their sense that the industry is primarily responsible for any food safety outbreaks. Moreover, it is clear that certain segments of the population in all samples feel strongly about the importance of farm to fork traceability in beef; thus, policymakers may wish to consider extending traceability beyond the point of slaughter as a way of encouraging beef sales in Canada.

  18. Quality Assurance Plan for the Upper East Fork Poplar Creek Characterization Area, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-12-01

    This quality assurance plan summarizes requirements for conducting work on the Upper East 9 Fork Poplar Creek (UEFPC) Characterization Area (CA). The reader is referred to the Expanded Task Work Agreement for Upper East Fork Poplar Creek Characterization Area, Remedial Investigation/Feasibility Study (RI/FS) for details regarding the activities, roles, and responsibilities summarized here. UEFPC is designated a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) site and thus requires a remedial investigation (RI) and a feasibility study (FS). The RI objectives are to evaluate the nature and extent of known and suspected contaminates, to provide data to perform baseline ecological and human health risk assessments, and to support development and evaluation of remedial alternatives for the FS,. Existing data will be used as much as possible. Additional sampling may be required to fill data gaps. The goal of the RI is to prioritize the major sources of contaminants to exit pathways and to understand their characteristics for risk characterization and development of remedial alternatives. The FS objectives are to investigate technologies and develop and evaluate alternatives based on 2031 CERCLA guidance

  19. Mutation spectrum of fork-head transcriptional factor gene (FOXL2) in Indian Blepharophimosis Ptosis Epicanthus Inversus Syndrome (BPES) patients.

    Science.gov (United States)

    Kaur, Inderjeet; Hussain, Avid; Naik, Milind N; Murthy, Ramesh; Honavar, Santosh G

    2011-06-01

    The fork-head transcription factor gene (FOXL2) gene has been implicated in Blepharophimosis Ptosis Epicanthus Inversus Syndrome (BPES) type I and type II. The authors aimed to evaluate the involvement of FOXL2 in familial and sporadic cases of BPES in an Indian cohort. The present cohort comprised clinically well-characterised BPES cases that included six affected families, two sporadic cases and 60 unaffected normal controls. The 5' untranslated and coding region of FOXL2 was screened by resequencing and confirmed by restriction digestion. Further, genotype-phenotype correlations were done to understand the implications of the observed mutation. Six mutations were observed in eight cases (87.5%). These included a novel deletion (c.860delC), three previously reported duplications (c.663-692dup 30, c.672-701dup30 and c.843-859dup17), a frame shift (c.804dupC) and a homozygous missense mutation (p.E69K). The p.E69k mutation was seen in both heterozygous and homozygous form in a large four-generational family, and disease severity was found to be directly linked to the allelic dosage. Two SNPs (c.501C→T, c.536C→G) were also noted. An unusual coexistence of polycystic ovarian disease (PCOD) with BPES was also seen in one of the families. Mutations in the region downstream of the fork-head domain were predominantly responsible for BPES among Indian patients.

  20. Best management practices plan for the Lower East Fork Poplar Creek remedial action project, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-08-01

    The U.S. Department of Energy (DOE) has three major operating facilities on the DOE Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee: the Oak Ridge Y-12 Plant, the K-25 Site, and the Oak Ridge National Laboratory (ORNL) managed by Lockheed Martin Environmental Research Corporation. All facilities are managed by Lockheed Martin Energy Systems, Incorporated (Energy Systems) for the DOE. The Y-12 Plant is adjacent to the city of Oak Ridge and is also upstream from Oak Ridge along East Fork Poplar Creek. The portion of the creek downstream from the Y-12 Plant is Lower East Fork Poplar Creek (LEFPC). This project will remove mercury-contaminated soils from the LEFPC floodplain, transport the soils to Industrial Landfill V (ILF-V), and restore any affected areas. This project contains areas that were designated in 1989 as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) site. The site includes DOE property and portions of commercial, residential, agricultural, and miscellaneous areas within the city of Oak Ridge

  1. A High Sensitivity Preamplifier for Quartz Tuning Forks in QEPAS (Quartz Enhanced PhotoAcoustic Spectroscopy Applications

    Directory of Open Access Journals (Sweden)

    Tomasz Starecki

    2017-11-01

    Full Text Available All the preamplifiers dedicated for Quartz Enhanced PhotoAcoustic Spectroscopy (QEPAS applications that have so far been reported in the literature have been based on operational amplifiers working in transimpedance configurations. Taking into consideration that QEPAS sensors are based on quartz tuning forks, and that quartz has a relatively high voltage constant and relatively low charge constant, it seems that a transimpedance amplifier is not an optimal solution. This paper describes the design of a quartz QEPAS sensor preamplifier, implemented with voltage amplifier configuration. Discussion of an electrical model of the circuit and preliminary measurements are presented. Both theoretical analysis and experiments show that use of the voltage configuration allows for a substantial increase of the output signal in comparison to the transimpedance circuit with the same tuning fork working in identical conditions. Assuming that the sensitivity of the QEPAS technique depends directly on the properties of the preamplifier, use of the voltage amplifier configuration should result in an increase of QEPAS sensitivity by one to two orders of magnitude.

  2. A High Sensitivity Preamplifier for Quartz Tuning Forks in QEPAS (Quartz Enhanced PhotoAcoustic Spectroscopy) Applications.

    Science.gov (United States)

    Starecki, Tomasz; Wieczorek, Piotr Z

    2017-11-03

    All the preamplifiers dedicated for Quartz Enhanced PhotoAcoustic Spectroscopy (QEPAS) applications that have so far been reported in the literature have been based on operational amplifiers working in transimpedance configurations. Taking into consideration that QEPAS sensors are based on quartz tuning forks, and that quartz has a relatively high voltage constant and relatively low charge constant, it seems that a transimpedance amplifier is not an optimal solution. This paper describes the design of a quartz QEPAS sensor preamplifier, implemented with voltage amplifier configuration. Discussion of an electrical model of the circuit and preliminary measurements are presented. Both theoretical analysis and experiments show that use of the voltage configuration allows for a substantial increase of the output signal in comparison to the transimpedance circuit with the same tuning fork working in identical conditions. Assuming that the sensitivity of the QEPAS technique depends directly on the properties of the preamplifier, use of the voltage amplifier configuration should result in an increase of QEPAS sensitivity by one to two orders of magnitude.

  3. Helicase and Polymerase Move Together Close to the Fork Junction and Copy DNA in One-Nucleotide Steps

    Directory of Open Access Journals (Sweden)

    Manjula Pandey

    2014-03-01

    Full Text Available By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading-strand synthesis, taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound/copied. The cooperative catalysis enables the helicase and polymerase to move at a uniformly fast rate without guanine:cytosine (GC dependency or idling with futile NTP hydrolysis. We show that the helicase and polymerase are located close to the replication fork junction. This architecture enables the polymerase to use its strand-displacement synthesis to increase the unwinding rate, whereas the helicase aids this process by translocating along single-stranded DNA and trapping the unwound bases. Thus, in contrast to the helicase-only unwinding model, our results suggest a model in which the helicase and polymerase are moving in one-nucleotide steps, DNA synthesis drives fork unwinding, and a role of the helicase is to trap the unwound bases and prevent DNA reannealing.

  4. DNA polymerase η modulates replication fork progression and DNA damage responses in platinum-treated human cells

    Science.gov (United States)

    Sokol, Anna M.; Cruet-Hennequart, Séverine; Pasero, Philippe; Carty, Michael P.

    2013-11-01

    Human cells lacking DNA polymerase η (polη) are sensitive to platinum-based cancer chemotherapeutic agents. Using DNA combing to directly investigate the role of polη in bypass of platinum-induced DNA lesions in vivo, we demonstrate that nascent DNA strands are up to 39% shorter in human cells lacking polη than in cells expressing polη. This provides the first direct evidence that polη modulates replication fork progression in vivo following cisplatin and carboplatin treatment. Severe replication inhibition in individual platinum-treated polη-deficient cells correlates with enhanced phosphorylation of the RPA2 subunit of replication protein A on serines 4 and 8, as determined using EdU labelling and immunofluorescence, consistent with formation of DNA strand breaks at arrested forks in the absence of polη. Polη-mediated bypass of platinum-induced DNA lesions may therefore represent one mechanism by which cancer cells can tolerate platinum-based chemotherapy.

  5. Protect and Restore Red River Watershed, 2007-2008 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Bransford, Stephanie [Nez Perce Tribe Fisheries/Watershed Program

    2009-05-04

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. The Nez Perce Tribe (NPT) and the Nez Perce National Forest (NPNF) have formed a partnership in completing watershed restoration activities, and through this partnership more work is accomplished by sharing funding and resources in our effort. The Nez Perce Tribe began watershed restoration projects within the Red River Watershed of the South Fork Clearwater River in 2001. Progress has been made in restoring the watershed through road decommissioning and culvert replacement. From completing a watershed assessment to two NEPA efforts and a final stream restoration design, we will begin the effort of restoring the mainstem channel of Red River to provide spawning and rearing habitat for anadromous and resident fish species. Roads have been surveyed and prioritized for removal or improvement as well as culverts being prioritized for replacement to accommodate fish passage throughout the watershed. Another major, and extremely, important component of this project is the Red River Meadow Conservation Easement. We have begun the process of pursuing a conservation easement on approximately 270 acres of prime meadow habitat (Red River runs through this meadow and is prime spawning and rearing habitat).

  6. Application of ELJ to create and maintain side channels in a dynamic gravel bed river

    Science.gov (United States)

    Crabbe, E.; Crowe Curran, J.; Ockelford, A.

    2017-12-01

    Braided and anastomosing rivers create and maintain a large amount of side channel habitat. Unfortunately, many rivers that were once multi-channel rivers have been constrained to single thread channels as a consequence of land use changes that occurred in the 19th and 20th centuries or earlier. An increasingly common management goal today is the re-creation of self-maintaining side and tributary habitat through as natural means as possible. This work examines the geomorphic history of one such channel and the success of recent rehabilitation efforts. Our case study comes from the South Fork Nooksack River in the Cascades Range in Washington State. The Nooksack River is a gravel and sand bed channel with a snowmelt dominated hydrograph. Engineered log jams (ELJ) have been employed to direct flow into side and chute channels with the larger goals of increasing overall channel complexity and salmon spawning opportunities. ELJs have been constructed on the channel since the 2000s, and the ELJs in the study reaches range in age up to 10 years. The size and design of individual jams within the reach vary, enabling a comparison between jam types. ELJs are evaluated for their ability to maintain gravel bar locations and open tributary channels through the snowmelt season over the reach scale. Additional goals of trapping wood onto the jams and existing bars, stabilizing channel banks, and allowing for the growth of bar vegetation are also examined.

  7. Variation of Probable Maximum Precipitation in Brazos River Basin, TX

    Science.gov (United States)

    Bhatia, N.; Singh, V. P.

    2017-12-01

    The Brazos River basin, the second-largest river basin by area in Texas, generates the highest amount of flow volume of any river in a given year in Texas. With its headwaters located at the confluence of Double Mountain and Salt forks in Stonewall County, the third-longest flowline of the Brazos River traverses within narrow valleys in the area of rolling topography of west Texas, and flows through rugged terrains in mainly featureless plains of central Texas, before its confluence with Gulf of Mexico. Along its major flow network, the river basin covers six different climate regions characterized on the basis of similar attributes of vegetation, temperature, humidity, rainfall, and seasonal weather changes, by National Oceanic and Atmospheric Administration (NOAA). Our previous research on Texas climatology illustrated intensified precipitation regimes, which tend to result in extreme flood events. Such events have caused huge losses of lives and infrastructure in the Brazos River basin. Therefore, a region-specific investigation is required for analyzing precipitation regimes along the geographically-diverse river network. Owing to the topographical and hydroclimatological variations along the flow network, 24-hour Probable Maximum Precipitation (PMP) was estimated for different hydrologic units along the river network, using the revised Hershfield's method devised by Lan et al. (2017). The method incorporates the use of a standardized variable describing the maximum deviation from the average of a sample scaled by the standard deviation of the sample. The hydrometeorological literature identifies this method as more reasonable and consistent with the frequency equation. With respect to the calculation of stable data size required for statistically reliable results, this study also quantified the respective uncertainty associated with PMP values in different hydrologic units. The corresponding range of return periods of PMPs in different hydrologic units was

  8. Comparison of the yields of downstream migrant salmonids before and after logging and road construction on the South Fork Caspar Creek, Mendocino County

    Science.gov (United States)

    David S. Graves; James W. Burns

    1970-01-01

    Yields of juvenile steelhead rainbow trout Salmo gairdnerii gairdnerii) and silver salmon (Oncorhynchus kisutch) emigrants were compared in South Fork Caspar Creek, a small coastal stream in Mendocino County, California, before and after construction of a logging road along the stream in the summer of 1967. Numbers, lengths, and age class structures were compared....

  9. Sampling and analysis plan for treatment water and creek water for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This document provides the Environmental Restoration Program with information about the methodology, organizational structure, quality assurance and health and safety practices to be employed during the water sampling and analysis activities associated with the remediation of the Lower East Fork Poplar Creek Operable Unit during remediation of the National Oceanic and Atmospheric Administration and Bruner sites

  10. Sampling and analysis plan for treatment water and creek water for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    This document provides the Environmental Restoration Program with information about the methodology, organizational structure, quality assurance and health and safety practices to be employed during the water sampling and analysis activities associated with the remediation of the Lower East Fork Poplar Creek Operable Unit during remediation of the National Oceanic and Atmospheric Administration and Bruner sites.

  11. High-resolution digital elevation model of lower Cowlitz and Toutle Rivers, adjacent to Mount St. Helens, Washington, based on an airborne lidar survey of October 2007

    Science.gov (United States)

    Mosbrucker, Adam

    2015-01-01

    The lateral blast, debris avalanche, and lahars of the May 18th, 1980, eruption of Mount St. Helens, Washington, dramatically altered the surrounding landscape. Lava domes were extruded during the subsequent eruptive periods of 1980–1986 and 2004–2008. More than three decades after the emplacement of the 1980 debris avalanche, high sediment production persists in the Toutle River basin, which drains the northern and western flanks of the volcano. Because this sediment increases the risk of flooding to downstream communities on the Toutle and lower Cowlitz Rivers, the U.S. Army Corps of Engineers (USACE), under the direction of Congress to maintain an authorized level of flood protection, continues to monitor and mitigate excess sediment in North and South Fork Toutle River basins to help reduce this risk and to prevent sediment from clogging the shipping channel of the Columbia River. From October 22–27, 2007, Watershed Sciences, Inc., under contract to USACE, collected high-precision airborne lidar (light detection and ranging) data that cover 273 square kilometers (105 square miles) of lower Cowlitz and Toutle River tributaries from the Columbia River at Kelso, Washington, to upper North Fork Toutle River (below the volcano's edifice), including lower South Fork Toutle River. These data provide a digital dataset of the ground surface, including beneath forest cover. Such remotely sensed data can be used to develop sediment budgets and models of sediment erosion, transport, and deposition. The U.S. Geological Survey (USGS) used these lidar data to develop digital elevation models (DEMs) of the study area. DEMs are fundamental to monitoring natural hazards and studying volcanic landforms, fluvial and glacial geomorphology, and surface geology. Watershed Sciences, Inc., provided files in the LASer (LAS) format containing laser returns that had been filtered, classified, and georeferenced. The USGS produced a hydro-flattened DEM from ground-classified points at

  12. Environmental Assessment Addressing Privatization of Military Family Housing at Grand Forks Air Force Base, North Dakota

    Science.gov (United States)

    2011-05-01

    falls within a range of 10 to 25 dBA for normal hearing. The threshold of pain occurs at the upper boundary of audibility, which is normally in the...aquifer is found in the Ordovician-aged Red River Formation. Yield varies depending on joints and fractures within the formation, and the...oc ia tio n an d th e N at io na l T rib al E nv iro nm en ta l C ou nc il. T he e m is si on fa ct or is a ss um ed to e nc om pa ss a

  13. Allegheny County Major Rivers

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of major rivers that flow through Allegheny County. These shapes have been taken from the Hydrology dataset. The Ohio River,...

  14. Environmental restoration of mercury contamination of East Fork Poplar Creek at the Department of Energy's Oak Ridge, Tennessee, reservation

    International Nuclear Information System (INIS)

    Page, D.G.

    1995-01-01

    During the open-quotes Cold Warclose quotes era, approximately 239,000 pounds of mercury were released from the Y-12 Nuclear Weapons Plant to the East Fork Poplar Creek watershed. As a result, approximately 75 tons of the contaminant resides within the floodplain soils beyond the confines of the DOE reservation, a Federal Superfund Site. The EFPC watershed encompasses multiple land uses whose ownership varies from private citizens, municipal government, and federal government. DOE, in cooperation with the State of Tennessee and EPA, proposes to clean up the contamination to a risk based standard of 400 ppm. This level has been determined to be protective of human health and the environment. The remedial process and development of the remedial alternative are the result of close interagency cooperation between the State, EPA, U.S. Fish ampersand Wildlife Service, and the Army Corps of Engineers. This case study outlines that process

  15. DNA Replication Control During Drosophila Development: Insights into the Onset of S Phase, Replication Initiation, and Fork Progression

    Science.gov (United States)

    Hua, Brian L.; Orr-Weaver, Terry L.

    2017-01-01

    Proper control of DNA replication is critical to ensure genomic integrity during cell proliferation. In addition, differential regulation of the DNA replication program during development can change gene copy number to influence cell size and gene expression. Drosophila melanogaster serves as a powerful organism to study the developmental control of DNA replication in various cell cycle contexts in a variety of differentiated cell and tissue types. Additionally, Drosophila has provided several developmentally regulated replication models to dissect the molecular mechanisms that underlie replication-based copy number changes in the genome, which include differential underreplication and gene amplification. Here, we review key findings and our current understanding of the developmental control of DNA replication in the contexts of the archetypal replication program as well as of underreplication and differential gene amplification. We focus on the use of these latter two replication systems to delineate many of the molecular mechanisms that underlie the developmental control of replication initiation and fork elongation. PMID:28874453

  16. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-09-01

    The Lower East Fork Poplar Creek (LEFPC) extends fourteen (14) miles through Oak Ridge, TN. The Creek sediments and surrounding floodplain soils are contaminated with mercury compounds. This project involved a comprehensive pilot demonstration on thermal desorption of these soils to validate the feasibility of the remedial technology which had been identified in previous studies. Thermal desorption is a technology that utilizes heating or drying of soils to induce volatilization of contaminants. These contaminants are then vaporized and either incinerated or condensed in the second stage of desorption. Mercury (Hg), which was the principal contaminate of concern, was collected by condensers in a vapor collection system. This type of system insured that the toxic mercury vapors did not escape to the atmosphere.

  17. Determination of the static spring constant of electrically-driven quartz tuning forks with two freely oscillating prongs

    International Nuclear Information System (INIS)

    González, Laura; Oria, Roger; Botaya, Luis; Puig-Vidal, Manel; Otero, Jorge

    2015-01-01

    Quartz tuning forks have become popular in nanotechnology applications, especially as sensors for scanning probe microscopy. The sensor’s spring constant and the oscillation amplitude are required parameters to evaluate the tip-sample forces; however, there is certain controversy within the research community as to how to arrive at a value for the static spring constant of the device when working in shear mode. Here, we present two different methods based on finite element simulations, to determine the value of the spring constant of the sensors: the amplitude and Cleveland methods. The results obtained using these methods are compared to those using the geometrical method, and show that the latter overestimates the spring constant of the device. (paper)

  18. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    International Nuclear Information System (INIS)

    1994-09-01

    The Lower East Fork Poplar Creek (LEFPC) extends fourteen (14) miles through Oak Ridge, TN. The Creek sediments and surrounding floodplain soils are contaminated with mercury compounds. This project involved a comprehensive pilot demonstration on thermal desorption of these soils to validate the feasibility of the remedial technology which had been identified in previous studies. Thermal desorption is a technology that utilizes heating or drying of soils to induce volatilization of contaminants. These contaminants are then vaporized and either incinerated or condensed in the second stage of desorption. Mercury (Hg), which was the principal contaminate of concern, was collected by condensers in a vapor collection system. This type of system insured that the toxic mercury vapors did not escape to the atmosphere

  19. Groundwater quality assessment for the Upper East Fork Poplar Creek Hydrogeologic Regime at the Y-12 Plant

    International Nuclear Information System (INIS)

    1992-08-01

    This report contains an evaluation of the groundwater quality data obtained during the 1991 calendar year at several hazardous and non-hazardous waste management facilities and underground storage tanks (USTs) associated with the US Department of Energy Y-12 Plant. These sites are within the Upper East Fork Poplar Creek Hydrogeologic Regime (UEFPCHR), which is one of three regimes defined for the purposes of groundwater and surfacewater quality monitoring. Section 2.0 of this report contains background information regarding groundwater monitoring at the waste-management sites and USTs located in the UEFPCHR. An overview of the hydrogeologic system in the UEFPCHR is provided in Section 3.0. A discussion of the interpretive assumptions used in evaluating the 1991 assessment data, and detailed descriptions of groundwater quality are presented in Section 4.0. Findings of the 1991 monitoring program are summarized in Section 5.0. Proposed modifications to the groundwater quality monitoring program in the UEFPCHR are presented

  20. Flowing with Rivers

    Science.gov (United States)

    Anderson, Heather

    2004-01-01

    This article describes a lesson in which students compare how artists have depicted rivers in paintings, using different styles, compositions, subject matter, colors, and techniques. They create a watercolor landscape that includes a river. Students can learn about rivers by studying them on site, through environmental study, and through works of…

  1. An evaluation of surgical outcome of bilateral cleft lip surgery using a modified Millard′s (Fork Flap technique

    Directory of Open Access Journals (Sweden)

    W L Adeyemo

    2013-01-01

    Full Text Available Background: The central third of the face is distorted by the bilateral cleft of the lip and palate and restoring the normal facial form is one of the primary goals for the reconstructive surgeons. The history of bilateral cleft lip repair has evolved from discarding the premaxilla and prolabium and approximating the lateral lip elements to a definitive lip and primary cleft nasal repair utilising the underlying musculature. The aim of this study was to review surgical outcome of bilateral cleft lip surgery (BCLS done at the Lagos University Teaching Hospital. Materials and Methods: A review of all cases of BCLS done between January 2007 and December 2012 at the Lagos University Teaching Hospital was done. Data analysis included age and sex of patients, type of cleft deformity and type of surgery (primary or secondary and whether the cleft deformity was syndromic and non-syndromic. Techniques of repair, surgical outcome and complications were also recorded. Results: A total of 39 cases of BCLS involving 21 males and 18 females were done during the period. This constituted 10% (39/390 of all cases of cleft surgery done during the period. There were 5 syndromic and 34 non-syndromic cases. Age of patients at time of surgery ranged between 3 months and 32 years. There were 24 bilateral cleft lip and palate deformities and 15 bilateral cleft lip deformities. Thirty-one of the cases were primary surgery, while 8 were secondary (revision surgery. The most common surgical technique employed was modified Fork flap (Millard technique, which was employed in 37 (95% cases. Conclusion: Bilateral cleft lip deformity is a common cleft deformity seen in clinical practice, surgical repair of which can be a challenge to an experienced surgeon. A modified Fork flap technique for repair of bilateral cleft lip is a reliable and versatile technique associated with excellent surgical outcome.

  2. Phase 2 confirmatory sampling data report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-01

    A Remedial Investigation of East Fork Poplar Creek (EFPC) concluded that mercury is the principal contaminant of concern in the EFPC floodplain. The highest concentrations of mercury were found to be in a visually distinct black layer of soil that typically lies 15 to 30 cm (6 to 12 in.) below the surface. Mercury contamination was found to be situated in distinct areas along the floodplain, and generally at depths > 20 cm (8 in.) below the surface. In accordance with Comprehensive, Environmental Response, Compensation, and Liability Act (CERCLA), a feasibility study was prepared to assess alternatives for remediation, and a proposed plan was issued to the public in which a preferred alternative was identified. In response to public input, the plan was modified and US Department of Energy (DOE) issued a Record of Decision in 1995 committing to excavating all soil in the EFPC floodplain exceeding a concentration of 400 parts per million (ppm) of mercury. The Lower East Fork Poplar Creek (LEFPC) remedial action (RA) focuses on the stretch of EFPC flowing from Lake Reality at the Y-12 Plant, through the city of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation (ORR) and its associated floodplain. Specific areas were identified that required remediation at the National Oceanographic and Atmospheric Administration (NOAA) Site along Illinois Avenue and at the Bruner Site along the Oak Ridge Turnpike. The RA was conducted in two separate phases. Phase 2, conducted from February to October 1997, completed the remediation efforts at the NOAA facility and fully remediated the Bruner Site. During both phases, data were collected to show that the remedial efforts performed at the NOAA and Bruner sites were successful in implementing the Record of Decision and had no adverse impact on the creek water quality or the city of Oak Ridge publicly owned treatment works.

  3. Phase II confirmatory sampling data report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1998-01-01

    A Remedial Investigation of East Fork Poplar Creek (EFPC) concluded that mercury is the principal contaminant of concern in the EFPC floodplain. The highest concentrations of mercury were found to be in a visually distinct black layer of soil that typically lies 15 to 30 cm (6 to 12 in.) below the surface. Mercury contamination was found to be situated in distinct areas along the floodplain, and generally at depths > 20 cm (8 in.) below the surface. In accordance with Comprehensive, Environmental Response, Compensation, and Liability Act (CERCLA), a feasibility study was prepared to assess alternatives for remediation, and a proposed plan was issued to the public in which a preferred alternative was identified. In response to public input, the plan was modified and US Department of Energy (DOE) issued a Record of Decision in 1995 committing to excavating all soil in the EFPC floodplain exceeding a concentration of 400 parts per million (ppm) of mercury. The Lower East Fork Poplar Creek (LEFPC) remedial action (RA) focuses on the stretch of EFPC flowing from Lake Reality at the Y-12 Plant, through the city of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation (ORR) and its associated floodplain. Specific areas were identified that required remediation at the National Oceanographic and Atmospheric Administration (NOAA) Site along Illinois Avenue and at the Bruner Site along the Oak Ridge Turnpike. The RA was conducted in two separate phases. Phase 2, conducted from February to October 1997, completed the remediation efforts at the NOAA facility and fully remediated the Bruner Site. During both phases, data were collected to show that the remedial efforts performed at the NOAA and Bruner sites were successful in implementing the Record of Decision and had no adverse impact on the creek water quality or the city of Oak Ridge publicly owned treatment works

  4. Captive Rearing Program for Salmon River Chinook Salmon : Project Progress Report, 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Venditti, David A.

    2003-10-01

    During 2001, the Idaho Department of Fish and Game continued to develop techniques to rear chinook salmon Oncorhynchus tshawytscha to sexual maturity in captivity and to monitor their reproductive performance under natural conditions. Eyed-eggs were hydraulically collected from redds in the East Fork Salmon River (EFSR; N = 311) and the West Fork Yankee Fork Salmon River (WFYF; N = 272) to establish brood year 2001 culture cohorts. The eyed-eggs were incubated and reared by family group at the Eagle Fish Hatchery (Eagle). Juveniles collected the previous summer were PIT and elastomer tagged and vaccinated against vibrio Vibrio spp. and bacterial kidney disease prior to the majority of them being transferred to the National Marine Fisheries Service, Manchester Marine Experimental Station for saltwater rearing through maturity. Smolt transfers included 210 individuals from the Lemhi River (LEM), 242 from the WFYF, and 178 from the EFSR. Maturing fish transfers from Manchester to Eagle included 62 individuals from the LEM, 72 from the WFYF, and 27 from the EFSR. Additional water chilling capacity was added at Eagle in 2001 to test if spawn timing could be advanced by temperature manipulations, and adults from the LEM and WFYF were divided into chilled ({approx} 9 C) and ambient ({approx} 13.5 C) water temperature groups while at Eagle. Twenty-five mature females from the LEM (11 chilled, 14 ambient) were spawned in captivity with 23 males with the same temperature history in 2001. Water temperature group was not shown to affect the spawn timing of these females, but males did mature earlier. Egg survival to the eyed stage of development averaged 37.9% and did not differ significantly between the two temperature groups. A total of 8,154 eyed-eggs from these crosses were placed in in-stream incubators by personnel from the Shoshone-Bannock Tribe. Mature adults (N = 89) were released into the WFYF to evaluate their reproductive performance. After release, fish

  5. Plant water status relationships among major floodplain sites of the Flathead River, Montana

    Science.gov (United States)

    Lee, L.C.; Hinckley, T.M.; Scott, M.L.

    1985-01-01

    Water status measurements of dominant species from major floodplain plant community types of the North Fork Flathead River, Montana were used to test the accuracy of site moisture gradient relationships postulated from floristic ordinations and site water balance estimates. Analysis of variance tests showed significant differences among the average predawn xylem pressure potential (ψp) of species in several community types. However, additional analyses failed to indicate a significant degree of association between averaged predawn Yp measurements and either floristic ordination or site water balance results. Sixty eight percent of 22 trials comparing the diurnal average ψp of the same species in different community types on the same day were less negative for a species in the wetter community types as predicted by floristic ordinations. Similarly, 64% of the trials indicated that the diurnal average stomatal conductance was higher for a species in the wetter type. These results suggest that although a floodplain moisture gradient exists, it alone does not limit the distribution of floodplain plant communities in the North Fork.

  6. River basin administration

    Science.gov (United States)

    Management of international rivers and their basins is the focus of the Centre for Comparative Studies on (International) River Basin Administration, recently established at Delft University of Technology in the Netherlands. Water pollution, sludge, and conflicting interests in the use of water in upstream and downstream parts of a river basin will be addressed by studying groundwater and consumption of water in the whole catchment area of a river.Important aspects of river management are administrative and policy aspects. The Centre will focus on policy, law, planning, and organization, including transboundary cooperation, posing standards, integrated environmental planning on regional scale and environmental impact assessments.

  7. Operation of river systems. The Otra river

    International Nuclear Information System (INIS)

    Harby, A.; Vaskinn, K.A.; Wathne, M.; Heggenes, J.; Saltveit, S.J.

    1993-12-01

    The purpose of the project described in this report was to prepare an operative tool for making decisions about the operation of the power system on the river Otra (Norway) with regard to how this operation might affect the various users of the river system. Above all this affects fish, outdoor life and esthetic values. The connection between water quality and volume of discharge has been examined in a sub project. How suitable parts of the river are as habitats for trout has been simulated on a computer. From field investigation it is concluded that near the Steinfoss power station the physical conditions for trout depend on the operation of the river system. Outdoor life is not much affected downstream Vikeland. 11 refs., 22 figs., 2 tabs

  8. 76 FR 51887 - Safety Zone; Patuxent River, Patuxent River, MD

    Science.gov (United States)

    2011-08-19

    ...-AA00 Safety Zone; Patuxent River, Patuxent River, MD AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone during the ``NAS Patuxent River... held over certain waters of the Patuxent River adjacent to Patuxent River, Maryland from September 1...

  9. Application of Acoustic and Optic Methods for Estimating Suspended-Solids Concentrations in the St. Lucie River Estuary, Florida

    Science.gov (United States)

    Patino, Eduardo; Byrne, Michael J.

    2004-01-01

    Acoustic and optic methods were applied to estimate suspended-solids concentrations in the St. Lucie River Estuary, southeastern Florida. Acoustic Doppler velocity meters were installed at the North Fork, Speedy Point, and Steele Point sites within the estuary. These sites provide varying flow, salinity, water-quality, and channel cross-sectional characteristics. The monitoring site at Steele Point was not used in the analyses because repeated instrument relocations (due to bridge construction) prevented a sufficient number of samples from being collected at the various locations. Acoustic and optic instruments were installed to collect water velocity, acoustic backscatter strength (ABS), and turbidity data that were used to assess the feasibility of estimating suspended-solids concentrations in the estuary. Other data collected at the monitoring sites include tidal stage, salinity, temperature, and periodic discharge measurements. Regression analyses were used to determine the relations of suspended-solids concentration to ABS and suspended-solids concentration to turbidity at the North Fork and Speedy Point sites. For samples used in regression analyses, measured suspended-solids concentrations at the North Fork and Speedy Point sites ranged from 3 to 37 milligrams per liter, and organic content ranged from 50 to 83 percent. Corresponding salinity for these samples ranged from 0.12 to 22.7 parts per thousand, and corresponding temperature ranged from 19.4 to 31.8 ?C. Relations determined using this technique are site specific and only describe suspended-solids concentrations at locations where data were collected. The suspended-solids concentration to ABS relation resulted in correlation coefficients of 0.78 and 0.63 at the North Fork and Speedy Point sites, respectively. The suspended-solids concentration to turbidity relation resulted in correlation coefficients of 0.73 and 0.89 at the North Fork and Speedy Point sites, respectively. The adequacy of the

  10. Salmonid Gamete Preservation in the Snake River Basin, 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Armstrong, Robyn; Kucera, Paul

    2002-06-01

    Steelhead (Oncorhynchus mykiss) and chinook salmon (Oncorhynchus tshawytscha) populations in the Northwest are decreasing. Genetic diversity is being lost at an alarming rate. Along with reduced population and genetic variability, the loss of biodiversity means a diminished environmental adaptability. The Nez Perce Tribe (Tribe) strives to ensure availability of genetic samples of the existing male salmonid population by establishing and maintaining a germplasm repository. The sampling strategy, initiated in 1992, has been to collect and preserve male salmon and steelhead genetic diversity across the geographic landscape by sampling within the major river subbasins in the Snake River basin, assuming a metapopulation structure existed historically. Gamete cryopreservation conserves genetic diversity in a germplasm repository, but is not a recovery action for listed fish species. The Tribe was funded in 2001 by the Bonneville Power Administration (BPA) and the U.S. Fish and Wildlife Service Lower Snake River Compensation Plan (LSRCP) to coordinate gene banking of male gametes from Endangered Species Act (ESA) listed steelhead and spring and summer chinook salmon in the Snake River basin. In 2001, a total of 398 viable chinook salmon semen samples from the Lostine River, Catherine Creek, upper Grande Ronde River, Lookingglass Hatchery (Imnaha River stock), Lake Creek, the South Fork Salmon River weir, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi Hatchery, and Sawtooth Hatchery (upper Salmon River stock) were cryopreserved. Also, 295 samples of male steelhead gametes from Dworshak Hatchery, Fish Creek, Grande Ronde River, Little Sheep Creek, Pahsimeroi Hatchery and Oxbow Hatchery were also cryopreserved. The Grande Ronde chinook salmon captive broodstock program stores 680 cryopreserved samples at the University of Idaho as a long-term archive, half of the total samples. A total of 3,206 cryopreserved samples from Snake River basin steelhead and

  11. Water Budgets of the Walker River Basin and Walker Lake, California and Nevada

    Science.gov (United States)

    Lopes, Thomas J.; Allander, Kip K.

    2009-01-01

    The Walker River is the main source of inflow to Walker Lake, a closed-basin lake in west-central Nevada. The only outflow from Walker Lake is evaporation from the lake surface. Between 1882 and 2008, upstream agricultural diversions resulted in a lake-level decline of more than 150 feet and storage loss of 7,400,000 acre-feet. Evaporative concentration increased dissolved solids from 2,500 to 17,000 milligrams per liter. The increase in salinity threatens the survival of the Lahontan cutthroat trout, a native species listed as threatened under the Endangered Species Act. This report describes streamflow in the Walker River basin and an updated water budget of Walker Lake with emphasis on the lower Walker River basin downstream from Wabuska, Nevada. Water budgets are based on average annual flows for a 30-year period (1971-2000). Total surface-water inflow to the upper Walker River basin upstream from Wabuska was estimated to be 387,000 acre-feet per year (acre-ft/yr). About 223,000 acre-ft/yr (58 percent) is from the West Fork of the Walker River; 145,000 acre-ft/yr (37 percent) is from the East Fork of the Walker River; 17,000 acre-ft/yr (4 percent) is from the Sweetwater Range; and 2,000 acre-ft/yr (less than 1 percent) is from the Bodie Mountains, Pine Grove Hills, and western Wassuk Range. Outflow from the upper Walker River basin is 138,000 acre-ft/yr at Wabuska. About 249,000 acre-ft/yr (64 percent) of inflow is diverted for irrigation, transpired by riparian vegetation, evaporates from lakes and reservoirs, and recharges alluvial aquifers. Stream losses in Antelope, Smith, and Bridgeport Valleys are due to evaporation from reservoirs and agricultural diversions with negligible stream infiltration or riparian evapotranspiration. Diversion rates in Antelope and Smith Valleys were estimated to be 3.0 feet per year (ft/yr) in each valley. Irrigated fields receive an additional 0.8 ft of precipitation, groundwater pumpage, or both for a total applied-water rate

  12. Cesium Ion Exchange Program at the Hanford River Protection Project Waste Treatment Plant

    International Nuclear Information System (INIS)

    CHARLES, NASH

    2004-01-01

    The River Protection Project - Hanford Tank Waste Treatment and Immobilization Plant will use cesium ion exchange to remove 137Cs from Low Activity Waste down to 0.3 Ci/m3 in the Immobilized LAW, ILAW product. The project baseline for cesium ion exchange is the elutable SuperLig, R, 644, SL-644, resin registered trademark of IBC Advanced Technologies, Inc., American Fork, UT or the Department of Energy approved equivalent. SL-644 is solely available through IBC Advanced Technologies. To provide an alternative to this sole-source resin supply, the RPP--WTP initiated a three-stage process for selection and qualification of an alternative ion exchange resin for cesium removal in the RPPWTP. It was recommended that resorcinol formaldehyde RF be pursued as a potential alternative to SL-644

  13. John Day River Subbasin Fish Habitat Enhancement Project, 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Powell, Russ M.; Delano, Kenneth H.; Jerome, James P.

    2002-07-01

    Work undertaken in 2001 included: (1) 3335 structure posts were pounded on six new projects thereby protecting 10 miles of stream (2) Completion of 1000 ft. of barbed wire fence and one watergap on the Middle Fork of the John Day River/ Forrest property. (3) Fence removal of 5010 ft. of barbed wire fence on the Meredith project. (4) Maintenance of all active project fences (66 miles), watergaps (76), spring developments (32) and plantings were checked and repairs performed. (5) Since the initiation of the Fish Habitat Project in 1984 we have 63.74 miles of stream protected using 106.78 miles of fence. With the addition of the Restoration and Enhancement Projects we have 180.64 miles of fence protecting 120.6 miles of stream.

  14. Final report from VFL Technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils. LEFPC appendices. Volume 6. Appendix VI-X

    International Nuclear Information System (INIS)

    1994-09-01

    This final report from VFL Technologies for the pilot-scale thermal treatment of lower East Fork Poplar Creek floodplain soils dated September 1994 contains LEFPC Appendices, Volume 6, Appendix VI - X. These appendices cover the following areas: chain of custody, miscellaneous process calculations (residence time and orifice plate calculations), waste management (mercury and radiation confirmatory testing before and after final verification run), health and safety (training, respirator fit test and radiation work permits), and transportation (soil receipt documentation)

  15. An Assessment of health risk associated with mercury in soil and sediment from East Fork Poplar Creek, Oak Ridge, Tennessee. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Revis, N.; Holdsworth, G.; Bingham, G.; King, A.; Elmore, J.

    1989-04-01

    This report presents results from a study conducted to determine the toxicity of Mercury in soils sediments samples. Mice were fed via diet, soils and sediment, from various locations along the East Fork Poplar creek. Tissue distribution of pollutants was determined at various intervals. The tissue level relative to toxicity was used to determine the effect of a complex matrix on the gastrointestinal absorption and tissue distribution of the pollutants (other pollutants included cadmium and selenium).

  16. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.

    Directory of Open Access Journals (Sweden)

    Audrey Costes

    2010-12-01

    Full Text Available We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome.

  17. Water-quality, bed-sediment, and biological data (October 2015 through September 2016) and statistical summaries of data for streams in the Clark Fork Basin, Montana

    Science.gov (United States)

    Dodge, Kent A.; Hornberger, Michelle I.; Turner, Matthew A.

    2018-03-30

    Water, bed sediment, and biota were sampled in selected streams from Butte to near Missoula, Montana, as part of a monitoring program in the upper Clark Fork Basin of western Montana. The sampling program was led by the U.S. Geological Survey, in cooperation with the U.S. Environmental Protection Agency, to characterize aquatic resources in the Clark Fork Basin, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were on the Clark Fork and selected tributaries. Water samples were collected periodically at 20 sites from October 2015 through September 2016. Bed-sediment and biota samples were collected once at 13 sites during August 2016.This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at sites from October 2015 through September 2016. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Samples for analysis of turbidity were collected at 13 sites, whereas samples for analysis of dissolved organic carbon were collected at 10 sites. In addition, samples for analysis of nitrogen (nitrate plus nitrite) were collected at two sites. Daily values of mean suspended-sediment concentration and suspended-sediment discharge were determined for three sites. Seasonal daily values of turbidity were determined for five sites. Bed-sediment data include trace-element concentrations in the fine-grained (less than 0.063 millimeter) fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of water-quality, bed-sediment, and biological data for sites in the upper Clark Fork Basin are provided for the period of record.

  18. Eleven-year trend in acetanilide pesticide degradates in the Iowa River, Iowa.

    Science.gov (United States)

    Kalkhoff, Stephen J; Vecchia, Aldo V; Capel, Paul D; Meyer, Michael T

    2012-01-01

    Trends in concentration and loads of acetochlor, alachlor, and metolachlor and their ethanasulfonic (ESA) and oxanilic (OXA) acid degradates were studied from 1996 through 2006 in the main stem of the Iowa River, Iowa and in the South Fork Iowa River, a small tributary near the headwaters of the Iowa River. Concentration trends were determined using the parametric regression model SEAWAVE-Q, which accounts for seasonal and flow-related variability. Daily estimated concentrations generated from the model were used with daily streamflow to calculate daily and yearly loads. Acetochlor, alachlor, metolachlor, and their ESA and OXA degradates were generally present in >50% of the samples collected from both sites throughout the study. Their concentrations generally decreased from 1996 through 2006, although the rate of decrease was slower after 2001. Concentrations of the ESA and OXA degradates decreased from 3 to about 23% yr. The concentration trend was related to the decreasing use of these compounds during the study period. Decreasing concentrations and constant runoff resulted in an average reduction of 10 to >3000 kg per year of alachlor and metolachlor ESA and OXA degradates being transported out of the Iowa River watershed. Transport of acetochlor and metolachlor parent compounds and their degradates from the Iowa River watershed ranged from <1% to about 6% of the annual application. These trends were related to the decreasing use of these compounds during the study period, but the year-to-year variability cannot explain changes in loads based on herbicide use alone. The trends were also affected by the timing and amount of precipitation. As expected, increased amounts of water moving through the watershed moved a greater percentage of the applied herbicides, especially the relatively soluble degradates, from the soils into the rivers through surface runoff, shallow groundwater inflow, and subsurface drainage. Copyright © by the American Society of Agronomy

  19. Lingering single-strand breaks trigger Rad51-independent homology-directed repair of collapsed replication forks in the polynucleotide kinase/phosphatase mutant of fission yeast.

    Directory of Open Access Journals (Sweden)

    Arancha Sanchez

    2017-09-01

    Full Text Available The DNA repair enzyme polynucleotide kinase/phosphatase (PNKP protects genome integrity by restoring ligatable 5'-phosphate and 3'-hydroxyl termini at single-strand breaks (SSBs. In humans, PNKP mutations underlie the neurological disease known as MCSZ, but these individuals are not predisposed for cancer, implying effective alternative repair pathways in dividing cells. Homology-directed repair (HDR of collapsed replication forks was proposed to repair SSBs in PNKP-deficient cells, but the critical HDR protein Rad51 is not required in PNKP-null (pnk1Δ cells of Schizosaccharomyces pombe. Here, we report that pnk1Δ cells have enhanced requirements for Rad3 (ATR/Mec1 and Chk1 checkpoint kinases, and the multi-BRCT domain protein Brc1 that binds phospho-histone H2A (γH2A at damaged replication forks. The viability of pnk1Δ cells depends on Mre11 and Ctp1 (CtIP/Sae2 double-strand break (DSB resection proteins, Rad52 DNA strand annealing protein, Mus81-Eme1 Holliday junction resolvase, and Rqh1 (BLM/WRN/Sgs1 DNA helicase. Coupled with increased sister chromatid recombination and Rad52 repair foci in pnk1Δ cells, these findings indicate that lingering SSBs in pnk1Δ cells trigger Rad51-independent homology-directed repair of collapsed replication forks. From these data, we propose models for HDR-mediated tolerance of persistent SSBs with 3' phosphate in pnk1Δ cells.

  20. Acute Smc5/6 depletion reveals its primary role in rDNA replication by restraining recombination at fork pausing sites.

    Directory of Open Access Journals (Sweden)

    Xiao P Peng

    2018-01-01

    Full Text Available Smc5/6, a member of the conserved SMC family of complexes, is essential for growth in most organisms. Its exact functions in a mitotic cell cycle are controversial, as chronic Smc5/6 loss-of-function alleles produce varying phenotypes. To circumvent this issue, we acutely depleted Smc5/6 in budding yeast and determined the first cell cycle consequences of Smc5/6 removal. We found a striking primary defect in replication of the ribosomal DNA (rDNA array. Each rDNA repeat contains a programmed replication fork barrier (RFB established by the Fob1 protein. Fob1 removal improves rDNA replication in Smc5/6 depleted cells, implicating Smc5/6 in the management of programmed fork pausing. A similar improvement is achieved by removing the DNA helicase Mph1 whose recombinogenic activity can be inhibited by Smc5/6 under DNA damage conditions. DNA 2D gel analyses further show that Smc5/6 loss increases recombination structures at RFB regions; moreover, mph1∆ and fob1∆ similarly reduce this accumulation. These findings point to an important mitotic role for Smc5/6 in restraining recombination events when protein barriers in rDNA stall replication forks. As rDNA maintenance influences multiple essential cellular processes, Smc5/6 likely links rDNA stability to overall mitotic growth.

  1. Electrospun single crystalline fork-like K2V8O21 as high-performance cathode materials for lithium-ion batteries

    Science.gov (United States)

    Hao, Pengfei; Zhu, Ting; Su, Qiong; Lin, Jiande; Cui, Rong; Cao, Xinxin; Wang, Yaping; Pan, Anqiang

    2018-06-01

    Single crystalline fork-like potassium vanadate (K2V8O21) has been successfully prepared through electrospinning combined with a subsequent annealing process. The as-obtained K2V8O21 forks show a unique layer-by-layer stacked structure with conductive carbon. When used as cathode materials for lithium-ion batteries, the as-prepared fork-like materials exhibit high specific discharge capacity and excellent cyclic stability. High specific discharge capacity of 200.2 mA h g-1 and 131.5 mA h g-1 can be delivered at the current densities of 50 mA g-1 and 500 mA g-1, respectively. Furthermore, the K2V8O21 electrodes exhibit excellent long-term cycling stability that maintain a capacity of 108.3 mA h g-1 after 300 cycles at 500 mA g-1 with a fading rate of only 0.054% per cycle, revealing their potential applications in next generation high-performance lithium-ion batteries.

  2. Down to the River

    DEFF Research Database (Denmark)

    Wessels, Josepha Ivanka

    2015-01-01

    Currently there is no coherent or sustainable water cooperation among the five states—Israel, Jordan, Lebanon, Palestinian territories and Syria—that share the Jordan River. Why do people not cooperate on sustainable river basin management, even if it seems the most rational course from the persp......Currently there is no coherent or sustainable water cooperation among the five states—Israel, Jordan, Lebanon, Palestinian territories and Syria—that share the Jordan River. Why do people not cooperate on sustainable river basin management, even if it seems the most rational course from...

  3. Investing in river health.

    Science.gov (United States)

    Bennett, J

    2002-01-01

    Rivers provide society with numerous returns. These relate to both the passive and extractive uses of the resources embodied in river environments. Some returns are manifest in the form of financial gains whilst others are non-monetary. For instance, rivers are a source of monetary income for those who harvest their fish. The water flowing in rivers is extracted for drinking and to water crops and livestock that in turn yield monetary profits. However, rivers are also the source of non-monetary values arising from biological diversity. People who use them for recreation (picnicking, swimming, boating) also receive non-monetary returns. The use of rivers to yield these returns has had negative consequences. With extraction for financial return has come diminished water quantity and quality. The result has been a diminished capacity of rivers to yield (non-extractive) environmental returns and to continue to provide extractive values. A river is like any other asset. With use, the value of an asset depreciates because its productivity declines. In order to maintain the productive capacity of their assets, managers put aside from their profits depreciation reserves that can be invested in the repair or replacement of those assets. Society now faces a situation in which its river assets have depreciated in terms of their capacity to provide monetary and non-monetary returns. An investment in river "repair" is required. But, investment means that society gives up something now in order to achieve some benefit in the future. Society thus has to grapple wih the choice between investing in river health and other investments--such as in hospitals, schools, defence etc. - as well as between investing in river health and current consumption--such as on clothes, food, cars etc. A commonly used aid for investment decision making in the public sector is benefit cost analysis. However, its usefulness in tackling the river investment problem is restricted because it requires all

  4. Hood River and Pelton Ladder monitoring and evaluation project and Hood River fish habitat project : annual progress report 1999-2000.; ANNUAL

    International Nuclear Information System (INIS)

    Lambert, Michael B.; McCanna, Joseph P.; Jennings, Mick

    2001-01-01

    The Hood River subbasin is home to four species of anadromous salmonids: chinook salmon (Oncorhynchus tshawytscha), coho salmon (Oncorhynchus kisutch), steelhead (Oncorhynchus mykiss), and sea run cutthroat trout (Salmo clarki). Indigenous spring chinook salmon were extirpated during the late 1960's. The naturally spawning spring chinook salmon currently present in the subbasin are progeny of Deschutes stock. Historically, the Hood River subbasin hatchery steelhead program utilized out-of-basin stocks for many years. Indigenous stocks of summer and winter steelhead were listed in March 1998 by National Marine Fisheries Service (NMFS) under the Endangered Species Act (ESA) as a ''Threatened'' Species along with similar genetically similar steelhead in the Lower Columbia Basin. This annual report summarizes work for two consecutive contract periods: the fiscal year (FY) 1999 contract period was 1 October, 1998 through 30 September, 1999 and 1 October, 1999 through 30 September, 2000 for FY 2000. Work implemented during FY 1999 and FY 2000 included (1) acclimation of hatchery spring chinook salmon and hatchery summer and winter steelhead smolts, (2) spring chinook salmon spawning ground surveys on the West Fork Hood River (3) genetic analysis of steelhead and cutthroat[contractual service with the ODFW], (4) Hood River water temperature studies, (5) Oak Springs Hatchery (OSH) and Round Butte Hatchery (RBH) coded-wire tagging and clipping evaluation, (6) preparation of the Hood River Watershed Assessment (Coccoli et al., December 1999) and the Fish Habitat Protection, Restoration, and Monitoring Plan (Coccoli et al., February 2000), (7) project implementation of early action habitat protection and restoration projects, (8) Pelton Ladder evaluation studies, (9) management oversight and guidance to BPA and ODFW engineering on HRPP facilities, and (10) preparation of an annual report summarizing project objectives for FY 1999 and FY 2000

  5. River Corridors (Jan 2, 2015)

    Data.gov (United States)

    Vermont Center for Geographic Information — River corridors are delineated to provide for the least erosive meandering and floodplain geometry toward which a river will evolve over time. River corridor maps...

  6. A Systems Evaluation of the Environmental Impact of the Aubrey Reservoir Project on Elm Fork of the Trinity River in North Texas.

    Science.gov (United States)

    1972-06-01

    exist within the reservoir site one automotive repair shop, one welding shop, one American Legion hut, one church, a baseball camp for boys, and a retreat...in providing benefits that are directed towards the buyer’s person or property. There exist one automotive re- pair shop, one welding shop, an...ignoring grandfather’s grave for years will be suddenly upset . How much they are affected will depend some on moving plans and assurances given. For

  7. Final Independent External Peer Review Report Supplemental Major Rehabilitation Evaluation Report for Center Hill Dam, Caney Fork River, DeKalb County, Tennessee

    Science.gov (United States)

    2013-09-25

    and outlet structure and the penstock system for the Rocky Mountain Pumped Storage Facility under both pump and turbine modes. He also was the...level will not overtop the main embankment dam but will encroach into the 3.6-feet of freeboard allotted for wind and wave run-up. This encroachment...In particular, short-term impacts on noise, air quality, water quality, migratory birds , fisheries, and traffic during construction have not been

  8. General Reevaluation and Supplement to Environmental Impact Statement for Flood Control and Related Purposes. Red and Red Lake Rivers at East Grand Forks, Minnesota.

    Science.gov (United States)

    1984-11-01

    areas that will be unloaded for Dank stability has identified one prehistoric and two historic archeologicai sites. Due to their present conditions...government bodies were brought into the study process through a wide-based public information program with regular communication on project matters . A

  9. Coastal coho salmon research in the West Fork Smith River: Patterns of coho salmon size and survival within a complex watershed

    Science.gov (United States)

    Effective habitat restoration planning requires the ability to anticipate fish population responses to altered habitats. The EPA has conducted network-scale research to document habitat-specific growth and survival of juvenile salmonids in a complex watershed. These findings ha...

  10. Stream seepage and groundwater levels, Wood River Valley, south-central Idaho, 2012-13

    Science.gov (United States)

    Bartolino, James R.

    2014-01-01

    Stream discharge and water levels in wells were measured at multiple sites in the Wood River Valley, south-central Idaho, in August 2012, October 2012, and March 2013, as a component of data collection for a groundwater-flow model of the Wood River Valley aquifer system. This model is a cooperative and collaborative effort between the U.S. Geological Survey and the Idaho Department of Water Resources. Stream-discharge measurements for determination of seepage were made during several days on three occasions: August 27–28, 2012, October 22–24, 2012, and March 27–28, 2013. Discharge measurements were made at 49 sites in August and October, and 51 sites in March, on the Big Wood River, Silver Creek, their tributaries, and nearby canals. The Big Wood River generally gains flow between the Big Wood River near Ketchum streamgage (13135500) and the Big Wood River at Hailey streamgage (13139510), and loses flow between the Hailey streamgage and the Big Wood River at Stanton Crossing near Bellevue streamgage (13140800). Shorter reaches within these segments may differ in the direction or magnitude of seepage or may be indeterminate because of measurement uncertainty. Additional reaches were measured on Silver Creek, the North Fork Big Wood River, Warm Springs Creek, Trail Creek, and the East Fork Big Wood River. Discharge measurements also were made on the Hiawatha, Cove, District 45, Glendale, and Bypass Canals, and smaller tributaries to the Big Wood River and Silver Creek. Water levels in 93 wells completed in the Wood River Valley aquifer system were measured during October 22–24, 2012; these wells are part of a network established by the U.S. Geological Survey in 2006. Maps of the October 2012 water-table altitude in the unconfined aquifer and the potentiometric-surface altitude of the confined aquifer have similar topology to those on maps of October 2006 conditions. Between October 2006 and October 2012, water-table altitude in the unconfined aquifer rose by

  11. The use of computed neutron coincidence counting with time interval analysis for the analysis of Fork-measurements on a fresh MOX-LWR fuel assembly under water

    Energy Technology Data Exchange (ETDEWEB)

    Baeten, P.; Bruggeman, M.; Carchon, R

    1998-06-01

    The objective of this study was to investigate the influence of different important parameters on measurement results for various fork-detectors. Computed Neutron Coincidence Counting (CNCC) with Time Interval Analysis (TIA) was used for this study. The performance of the electronics for the different fork-detectors was studied by investigating the deadtime perturbed zone of the Rossi-alpha distribution in TIA. The measurement revealed anomalies in the performance of the electronics of the IAEA BWR and LANL fork-detector. The IAEA PWR fork-detector functioned well and the deadtime parameter was calculated. The optimal setting for the pre delay was investigated and it was found that a pre delay of 10 micro seconds should be considered as an optimum between excluding from analysis data in the deadtime perturbed zone and keeping a high signal-to-noise ratio. For the shift register electronics used with the fork-detectors, a pre delay of only 4.5 micro seconds was used. The study of the pre delay and the deadtime showed that the calculated triples-rate is strongly dependent on these parameters. An accurate determination of the triple-rate in this type of measurements has proven to be quite difficult and requires proper operation of the electronics, a correct pre delay and an accurate deadtime correction formalism. By varying the boron concentration in water, the change of the decay time of the Rossi-alpha distribution was clearly observed. This change is due to the variation of the thermal multiplication. The variation of this decay time with the boron concentration proves that Boehnel's model for fast neutron multiplication is not valid under these measurement conditions and that a model for fast and thermal multiplication should be used in order to obtain unbiased measurement results. CNCC with TIA has proved to be a valuable tool in which parameter settings can be varied a posterori and the optimal setting can be determined for each measurement. Moreover, the

  12. INTEGRATED OUTCROP AND SUBSURFACE STUDIES OF THE INTERWELL ENVIRONMENT OF CARBONATE RESERVOIRS: CLEAR FORK (LEONARDIAN-AGE) RESERVOIRS, WEST TEXAS AND NEW MEXICO

    Energy Technology Data Exchange (ETDEWEB)

    F. Jerry Lucia

    2002-01-31

    This is the final report of the project ''Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonardian-Age) Reservoirs, West Texas and New Mexico'', Department of Energy contract no. DE-AC26-98BC15105 and is the third in a series of similar projects funded jointly by the U.S. Department of Energy and The University of Texas at Austin, Bureau of Economic Geology, Reservoir Characterization Research Laboratory for Carbonates. All three projects focus on the integration of outcrop and subsurface data for the purpose of developing improved methods for modeling petrophysical properties in the interwell environment. The first project, funded by contract no. DE-AC22-89BC14470, was a study of San Andres outcrops in the Algerita Escarpment, Guadalupe Mountains, Texas and New Mexico, and the Seminole San Andres reservoir, Permian Basin. This study established the basic concepts for constructing a reservoir model using sequence-stratigraphic principles and rock-fabric, petrophysical relationships. The second project, funded by contract no. DE-AC22-93BC14895, was a study of Grayburg outcrops in the Brokeoff Mountains, New Mexico, and the South Cowden Grayburg reservoir, Permian Basin. This study developed a sequence-stratigraphic succession for the Grayburg and improved methods for locating remaining hydrocarbons in carbonate ramp reservoirs. The current study is of the Clear Fork Group in Apache Canyon, Sierra Diablo Mountains, West Texas, and the South Wasson Clear Fork reservoir, Permian Basin. The focus was on scales of heterogeneity, imaging high- and low-permeability layers, and the impact of fractures on reservoir performance. In this study (1) the Clear Fork cycle stratigraphy is defined, (2) important scales of petrophysical variability are confirmed, (3) a unique rock-fabric, petrophysical relationship is defined, (4) a porosity method for correlating high-frequency cycles and defining rock

  13. The use of computed neutron coincidence counting with time interval analysis for the analysis of Fork-measurements on a fresh MOX-LWR fuel assembly under water

    International Nuclear Information System (INIS)

    Baeten, P.; Bruggeman, M.; Carchon, R.

    1998-06-01

    The objective of this study was to investigate the influence of different important parameters on measurement results for various fork-detectors. Computed Neutron Coincidence Counting (CNCC) with Time Interval Analysis (TIA) was used for this study. The performance of the electronics for the different fork-detectors was studied by investigating the deadtime perturbed zone of the Rossi-alpha distribution in TIA. The measurement revealed anomalies in the performance of the electronics of the IAEA BWR and LANL fork-detector. The IAEA PWR fork-detector functioned well and the deadtime parameter was calculated. The optimal setting for the pre delay was investigated and it was found that a pre delay of 10 micro seconds should be considered as an optimum between excluding from analysis data in the deadtime perturbed zone and keeping a high signal-to-noise ratio. For the shift register electronics used with the fork-detectors, a pre delay of only 4.5 micro seconds was used. The study of the pre delay and the deadtime showed that the calculated triples-rate is strongly dependent on these parameters. An accurate determination of the triple-rate in this type of measurements has proven to be quite difficult and requires proper operation of the electronics, a correct pre delay and an accurate deadtime correction formalism. By varying the boron concentration in water, the change of the decay time of the Rossi-alpha distribution was clearly observed. This change is due to the variation of the thermal multiplication. The variation of this decay time with the boron concentration proves that Boehnel's model for fast neutron multiplication is not valid under these measurement conditions and that a model for fast and thermal multiplication should be used in order to obtain unbiased measurement results. CNCC with TIA has proved to be a valuable tool in which parameter settings can be varied a posterori and the optimal setting can be determined for each measurement. Moreover, the

  14. Investigation of Increased Mercury Levels in the Fisheries of Lower East Fork Poplar Creek (LEFPC), Oak Ridge Reservation, Tennessee

    International Nuclear Information System (INIS)

    Byrne-Kelly, D.; Cornish, J.; Hart, A.; Southworth, G.; Simms, L.

    2006-01-01

    The DOE Western Environmental Technology Office (WETO) is supporting Oak Ridge's remediation efforts by performing this study. MSE Technology Applications, Inc. (MSE) has performed a series of literature reviews and bench-scale testing to further evaluate the mercury problem in the Lower East Fork Poplar Creek (LEFPC) at Oak Ridge. The primary problem is that total mercury (HgT) levels in LEFPC water decrease, while HgT levels in sunfish muscle tissue increase, with distance away from the National Security Complex (NSC), despite extensive source control efforts at the facility. Furthermore, dissolved methylmercury (d-MeHg) levels increase downstream from the NSC, especially during warm weather and/or high flow events. MSE performed four test series that focused on conversion of dissolved and colloidal forms of elemental mercury (Hg deg.A) to methyl mercury (MeHg) by algal-bacterial bio-films (periphyton) present in the stream-bed of LEFPC; MeHg production by these bio-films under anoxic versus oxic conditions was the critical measurement taken. The bench-scale testing for Phase I was completed November 2005. The final reporting and the planning for Phase II testing are in progress. (authors)

  15. Postremediation monitoring program baseline assessment report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Greeley, M.S. Jr.; Ashwood, T.L.; Kszos, L.A.; Peterson, M.J.; Rash, C.D.; Southworth, G.R. [Oak Ridge National Lab., TN (United States); Phipps, T.L. [CKY, Inc. (United States)

    1998-04-01

    Lower East Fork Poplar Creek (LEFPC) and its floodplain are contaminated with mercury (Hg) from ongoing and historical releases from the US Department of Energy (DOE) Oak Ridge Y-12 Plant. A remedial investigation and feasibility study of LEFPC resulted in the signing of a Record of Decision (ROD) in August 1995. In response to the ROD, soil contaminated with mercury above 400 mg/kg was removed from two sites in LEFPC and the floodplain during a recently completed remedial action (RA). The Postremediation Monitoring Program (PMP) outlined in the LEFPC Monitoring Plan was envisioned to occur in two phases: (1) a baseline assessment prior to remediation and (2) postremediation monitoring. The current report summarizes the results of the baseline assessment of soil, water, biota, and groundwater usage in LEFPC and its floodplain conducted in 1995 and 1996 by personnel of the Oak Ridge National Laboratory Biological Monitoring and Abatement Program (BMAP). This report also includes some 1997 data from contaminated sites that did not undergo remediation during the RA (i.e., sites where mercury is greater than 200 mg/kg but less than 400 mg/kg). The baseline assessment described in this document is distinct and separate from both the remedial investigation/feasibility study the confirmatory sampling conducted by SAIC during the RA. The purpose of the current assessment was to provide preremediation baseline data for the LEFPC PMP outlined in the LEFPC Monitoring Plan, using common approaches and techniques, as specified in that plan.

  16. Seasonal Alterations in Park Visitation, Amenity Use, and Physical Activity — Grand Forks, North Dakota, 2012–2013

    Science.gov (United States)

    Johnson, LuAnn

    2014-01-01

    Introduction Park amenities promote visitation and physical activity during summer. Physical activity declines during winter. Identifying park amenities that promote visitation during winter would increase year-round activity. The purpose of this study was to determine how park visitation, amenity choice, and physical activity intensity change across seasons. Methods Physical activity intensity of children and adults was assessed at 16 parks in Grand Forks, North Dakota, during summer and fall of 2012, and winter and spring of 2013. Results Park visitation was highest in spring and lowest in winter. Amenity use varied by season. Parks with water splash pads were visited more during summer, and playgrounds and open spaces were visited more during spring. Ice rinks were visited most in winter. Physical activity intensity was lowest in summer and highest in winter for each age group. The activity intensity observed for all young age groups ranged from 2.7 to 2.9 metabolic equivalents in summer and greater than 3 metabolic equivalents in all other seasons. Adults’ mean activity intensity was greater than 3 metabolic equivalents in winter. Conclusion Information on park visitation, amenity use, and activity intensity across seasons is valuable; it can be used when designing or redesigning parks in order to promote year-round physical activity. Redesigning parks in cold climates to include ice rinks, sledding hills, cross-country skiing, and indoor areas for physical activity would increase winter visitation and allow the park to serve as a year-round resource for physical activity. PMID:25211503

  17. Seasonal alterations in park visitation, amenity use, and physical activity--Grand Forks, North Dakota, 2012-2013.

    Science.gov (United States)

    Roemmich, James N; Johnson, LuAnn

    2014-09-11

    Park amenities promote visitation and physical activity during summer. Physical activity declines during winter. Identifying park amenities that promote visitation during winter would increase year-round activity. The purpose of this study was to determine how park visitation, amenity choice, and physical activity intensity change across seasons. Physical activity intensity of children and adults was assessed at 16 parks in Grand Forks, North Dakota, during summer and fall of 2012, and winter and spring of 2013. Park visitation was highest in spring and lowest in winter. Amenity use varied by season. Parks with water splash pads were visited more during summer, and playgrounds and open spaces were visited more during spring. Ice rinks were visited most in winter. Physical activity intensity was lowest in summer and highest in winter for each age group. The activity intensity observed for all young age groups ranged from 2.7 to 2.9 metabolic equivalents in summer and greater than 3 metabolic equivalents in all other seasons. Adults' mean activity intensity was greater than 3 metabolic equivalents in winter. Information on park visitation, amenity use, and activity intensity across seasons is valuable; it can be used when designing or redesigning parks in order to promote year-round physical activity. Redesigning parks in cold climates to include ice rinks, sledding hills, cross-country skiing, and indoor areas for physical activity would increase winter visitation and allow the park to serve as a year-round resource for physical activity.

  18. A quartz enhanced photo-acoustic gas sensor based on a custom tuning fork and a terahertz quantum cascade laser.

    Science.gov (United States)

    Patimisco, Pietro; Borri, Simone; Sampaolo, Angelo; Beere, Harvey E; Ritchie, David A; Vitiello, Miriam S; Scamarcio, Gaetano; Spagnolo, Vincenzo

    2014-05-07

    An innovative quartz enhanced photoacoustic (QEPAS) gas sensing system operating in the THz spectral range and employing a custom quartz tuning fork (QTF) is described. The QTF dimensions are 3.3 cm × 0.4 cm × 0.8 cm, with the two prongs spaced by ∼800 μm. To test our sensor we used a quantum cascade laser as the light source and selected a methanol rotational absorption line at 131.054 cm(-1) (∼3.93 THz), with line-strength S = 4.28 × 10(-21) cm mol(-1). The sensor was operated at 10 Torr pressure on the first flexion QTF resonance frequency of 4245 Hz. The corresponding Q-factor was 74 760. Stepwise concentration measurements were performed to verify the linearity of the QEPAS signal as a function of the methanol concentration. The achieved sensitivity of the system is 7 parts per million in 4 seconds, corresponding to a QEPAS normalized noise-equivalent absorption of 2 × 10(-10) W cm(-1) Hz(-1/2), comparable with the best result of mid-IR QEPAS systems.

  19. Second report on the Oak Ridge Y-12 Plant Biological Monitoring and Abatement Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Hinzman, R.L. [ed.; Adams, S.M. [Oak Ridge National Lab., TN (United States); Black, M.C. [Oklahoma State Univ., Stillwater, OK (United States)] [and others

    1993-06-01

    As stipulated in the National Pollutant Discharge Elimination System (NDPES) permit issued to the Oak Ridge Y-12 Plant on May 24, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for the receiving stream, East Fork Poplar Creek (EFPC). The objectives of BMAP are (1) to demonstrate that the current effluent limitations established for the Y-12 Plant protect the classified uses of EFPC (e.g., the growth and propagation of fish and aquatic life), as designated by the Tennessee Department of Environment and Conservation (TDEC) and (2) to document the ecological effects resulting from implementation of a Water Pollution Control Program that includes construction of several large wastewater treatment facilities. BMAP consists of four major tasks: (1) ambient toxicity testing; (2) bioaccumulation studies; (3) biological indicator studies; and (4) ecological surveys of stream communities, including periphyton (attached algae), benthic (bottom-dwelling) macroinvertebrates, and fish. This document, the second in a series of reports on the results of the Y-12 Plant BMAP, describes studies that were conducted between July 1986 and July 1988, although additional data collected outside this time period are included, as appropriate.

  20. Wear Analysis of Die Inserts in the Hot Forging Process of a Forked Type Forging Using Reverse Scanning Techniques

    Directory of Open Access Journals (Sweden)

    Łukasz Dworzak

    2017-12-01

    Full Text Available This article presents a wear analysis of die inserts used in the hot forging process of a forked forging (yoke, an element applied in steering systems of passenger vehicles. Studies involved the application of an original reverse scanning method intended for rapid and reliable wear analysis of forging tools (with complicated shape affording easy assessment without the need to dismount tools from the forging unit. The developed method involves analysis of progressive wear of forging tools based on measurements (scanning of forgings periodically collected from the process and constitutes a useful tool for measurement and testing. As the authors’ earlier works have demonstrated, the proposed new approach to analysis of tool wear with the application of reverse 3D scanning has proven successful in multiple instances in the case of axially symmetrical objects. The presented results of studies indicate that it is possible to utilize the expanded method to analyze the lifetime of forging tools, including tools with complex geometry. Application of the reverse scanning method allows for continuous and practical monitoring of the condition of forging tools over the course of the forging process and should have a positive impact on improving production output and reducing production costs.

  1. Second report on the Oak Ridge Y-12 Plant Biological Monitoring and Abatement Program for East Fork Poplar Creek

    International Nuclear Information System (INIS)

    Hinzman, R.L.; Black, M.C.

    1993-06-01

    As stipulated in the National Pollutant Discharge Elimination System (NDPES) permit issued to the Oak Ridge Y-12 Plant on May 24, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for the receiving stream, East Fork Poplar Creek (EFPC). The objectives of BMAP are (1) to demonstrate that the current effluent limitations established for the Y-12 Plant protect the classified uses of EFPC (e.g., the growth and propagation of fish and aquatic life), as designated by the Tennessee Department of Environment and Conservation (TDEC) and (2) to document the ecological effects resulting from implementation of a Water Pollution Control Program that includes construction of several large wastewater treatment facilities. BMAP consists of four major tasks: (1) ambient toxicity testing; (2) bioaccumulation studies; (3) biological indicator studies; and (4) ecological surveys of stream communities, including periphyton (attached algae), benthic (bottom-dwelling) macroinvertebrates, and fish. This document, the second in a series of reports on the results of the Y-12 Plant BMAP, describes studies that were conducted between July 1986 and July 1988, although additional data collected outside this time period are included, as appropriate

  2. Procedure for calculating estimated ultimate recoveries of Bakken and Three Forks Formations horizontal wells in the Williston Basin

    Science.gov (United States)

    Cook, Troy A.

    2013-01-01

    Estimated ultimate recoveries (EURs) are a key component in determining productivity of wells in continuous-type oil and gas reservoirs. EURs form the foundation of a well-performance-based assessment methodology initially developed by the U.S. Geological Survey (USGS; Schmoker, 1999). This methodology was formally reviewed by the American Association of Petroleum Geologists Committee on Resource Evaluation (Curtis and others, 2001). The EUR estimation methodology described in this paper was used in the 2013 USGS assessment of continuous oil resources in the Bakken and Three Forks Formations and incorporates uncertainties that would not normally be included in a basic decline-curve calculation. These uncertainties relate to (1) the mean time before failure of the entire well-production system (excluding economics), (2) the uncertainty of when (and if) a stable hyperbolic-decline profile is revealed in the production data, (3) the particular formation involved, (4) relations between initial production rates and a stable hyperbolic-decline profile, and (5) the final behavior of the decline extrapolation as production becomes more dependent on matrix storage.

  3. The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast.

    Science.gov (United States)

    Larsen, Nicolai B; Sass, Ehud; Suski, Catherine; Mankouri, Hocine W; Hickson, Ian D

    2014-04-07

    Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus, to specific DNA sequences called Ter. Here, we demonstrate that Tus-Ter modules also induce polar RF pausing when engineered into the Saccharomyces cerevisiae genome. This heterologous RF barrier is distinct from a number of previously characterized, protein-mediated, RF pause sites in yeast, as it is neither Tof1-dependent nor counteracted by the Rrm3 helicase. Although the yeast replisome can overcome RF pausing at Tus-Ter modules, this event triggers site-specific homologous recombination that requires the RecQ helicase, Sgs1, for its timely resolution. We propose that Tus-Ter can be utilized as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications.

  4. Groundwater quality assessment for the Upper East Fork Poplar Creek Hydrogeologic Regime at the Y-12 Plant

    International Nuclear Information System (INIS)

    1992-02-01

    This report contains groundwater quality data obtained during the 1991 calendar year at several waste management facilities and petroleum fuel underground storage tank (UST) sites associated with the Y-12 Plant. These sites are within the Upper East Fork Poplar Creek Hydrogeologic Regime (UEFPCHR), which is one of three regimes defined for the purposes of groundwater and surface-water quality monitoring and remediation. This report was prepared for informational purposes. Included are the analytical data for groundwater samples collected from selected monitoring wells during 1991 and the results for quality assurance/quality control (QA/QC) samples associated with each groundwater sample. This report also contains summaries of selected data, including ion-charge balances for each groundwater sample, a summary of analytical results for nitrate (a principle contaminant in the UEFPCHR), results of volatile organic compounds (VOCs) analyses validated using the associated QA/QC sample data, a summary of trace metal concentrations which exceeded drinking-water standards, and a summary of radiochemical analyses and associated counting errors

  5. Postremediation monitoring program baseline assessment report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Greeley, M.S. Jr.; Ashwood, T.L.; Kszos, L.A.; Peterson, M.J.; Rash, C.D.; Southworth, G.R.; Phipps, T.L.

    1998-04-01

    Lower East Fork Poplar Creek (LEFPC) and its floodplain are contaminated with mercury (Hg) from ongoing and historical releases from the US Department of Energy (DOE) Oak Ridge Y-12 Plant. A remedial investigation and feasibility study of LEFPC resulted in the signing of a Record of Decision (ROD) in August 1995. In response to the ROD, soil contaminated with mercury above 400 mg/kg was removed from two sites in LEFPC and the floodplain during a recently completed remedial action (RA). The Postremediation Monitoring Program (PMP) outlined in the LEFPC Monitoring Plan was envisioned to occur in two phases: (1) a baseline assessment prior to remediation and (2) postremediation monitoring. The current report summarizes the results of the baseline assessment of soil, water, biota, and groundwater usage in LEFPC and its floodplain conducted in 1995 and 1996 by personnel of the Oak Ridge National Laboratory Biological Monitoring and Abatement Program (BMAP). This report also includes some 1997 data from contaminated sites that did not undergo remediation during the RA (i.e., sites where mercury is greater than 200 mg/kg but less than 400 mg/kg). The baseline assessment described in this document is distinct and separate from both the remedial investigation/feasibility study the confirmatory sampling conducted by SAIC during the RA. The purpose of the current assessment was to provide preremediation baseline data for the LEFPC PMP outlined in the LEFPC Monitoring Plan, using common approaches and techniques, as specified in that plan

  6. Comparison of quartz tuning forks and AlN-based extensional microresonators for viscosity measurements in oil/fuel mixtures

    Science.gov (United States)

    Toledo, J.; Manzaneque, T.; Hernando-García, J.; Vazquez, J.; Ababneh, A.; Seidel, H.; Lapuerta, M.; Sánchez-Rojas, J. L.

    2013-05-01

    In-situ monitoring of the physical properties of liquids is of great interest in the automotive industry. For example, lubricants are subject to dilution with diesel fuel as a consequence of late-injection processes, which are necessary for regenerating diesel particulate filters. This dilution can be determined by tracking the viscosity and the density of the lubricant. Here we report the test of two in-plane movement based resonators to explore their capability to monitor oil dilution with diesel and biodiesel. One of the resonators is the commercially available millimeter-sized quartz tuning fork, working at 32.7 kHz. The second resonator is a state-of-the-art micron-sized AlN-based rectangular plate, actuated in the first extensional mode in the MHz range. Electrical impedance measurements were carried out to characterize the performance of the structures in various liquid media in a wide range of viscosities. These measurements were completed with the development of low-cost electronic circuits to track the resonance frequency and the quality factor automatically, these two parameters allow to obtain the viscosity of various fluids under investigation, as in the case of dilution of lubricant SAE 15W40 and biodiesel.

  7. Measurements on spent-fuel assemblies at Arkansas Nuclear One using the Fork system. Final report, January 1995

    International Nuclear Information System (INIS)

    Ewing, R.I.; Bronowski, D.R.; Bosler, G.E.; Siebelist, R.; Priore, J.; Hansford, C.H.; Sullivan, S.

    1997-03-01

    The Fork measurement system has been used to examine spent-fuel assemblies at the two reactors of Arkansas Nuclear One, operated by Entergy Operations, Inc. The Unit 1 reactor is a Babcock and Wilcox (B and W) design, and the Unit 2 reactor is a Combustion Engineering (CE) design. The neutron and gamma-ray emissions from individual spent-fuel assemblies were measured in the storage pools by raising each assembly pathway out of the storage rack and performing a measurement near the center of the assembly. The overall accuracy of the measurements after corrections is about 2%. Thirty-four assemblies were examined at Unit 1, and forty-one assemblies at Unit 2. The average deviation of the burnup measurements from the calibration was 3.0% at Unit 1 and 3.5% at Unit 2, indicating 2 to 3% random variation among the reactor records. There was no indication of clearly anomalous assemblies. Axial Scans of the variation in neutron and gamma ray emission were obtained by collecting data at several locations along the length of three assemblies at Unit 2. Two of these assemblies were nonstandard in that each contained a small neutron source. The sources were detected by the axial scans. The test program was a cooperative effort involving Sandia National Laboratories, Los Alamos National Laboratory, Entergy Operations, Inc., the Electric Power Research Institute, and the Office of Civilian Radioactive Waste Management of the US Department of Energy

  8. First report on the Oak Ridge Y-12 Plant Biological Monitoring and Abatement Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Loar, J.M.; Adams, S.M.; Allison, L.J.; Boston, H.L.; Huston, M.A.; McCarthy, J.F.; Smith, J.G.; Southworth, G.R.; Stewart, A.J. (Oak Ridge National Lab., TN (United States)); Black, M.C. (Oklahoma State Univ., Stillwater, OK (United States)); Gatz, A.J. Jr. (Ohio Wesleyan Univ., Delaware, OH (United States)); Hinzman, R.L. (Oak Ridge Research Inst., TN (United States)); Jimenez, B.D. (Puerto Rico Univ.,

    1992-07-01

    As stipulated in the National Pollutant Discharge Elimination System (NPDES) permit issued to the Oak Ridge Y-12 Plant on May 24, 1985, a Biological Monitoring and Abatement Program (BMAP) was developed for the receiving stream, East Fork Poplar Creek (EFPC). The objectives of the BMAP are (1) to demonstrate that the current effluent limitations established for the Oak Ridge Y-12 Plant protect the uses of EFPC (e.g., the growth and propagation of fish and aquatic life), as designated by the Tennessee Department of Environment and Conservation (TDEC) [formerly the Tennessee Department of Health and Environment (TDHE)], and (2) to document the ecological effects resulting from implementation of a water pollution control program that includes construction of several large wastewater treatment facilities. The BMAP consists of four major tasks: (1) ambient toxicity testing, (2) bioaccumulation studies, (3) biological indicator studies, and (4) ecological surveys of stream communities, including periphyton (attached algae), benthic macroinvertebrates, and fish. This document, the first in a series of reports on the results of the Y-12 Plant BMAP, describes studies that were conducted from May 1985 through September 1986.

  9. Design and Vibration Sensitivity Analysis of a MEMS Tuning Fork Gyroscope with an Anchored Diamond Coupling Mechanism

    Directory of Open Access Journals (Sweden)

    Yanwei Guan

    2016-04-01

    Full Text Available In this paper, a new micromachined tuning fork gyroscope (TFG with an anchored diamond coupling mechanism is proposed while the mode ordering and the vibration sensitivity are also investigated. The sense-mode of the proposed TFG was optimized through use of an anchored diamond coupling spring, which enables the in-phase mode frequency to be 108.3% higher than the anti-phase one. The frequencies of the in- and anti-phase modes in the sense direction are 9799.6 Hz and 4705.3 Hz, respectively. The analytical solutions illustrate that the stiffness difference ratio of the in- and anti-phase modes is inversely proportional to the output induced by the vibration from the sense direction. Additionally, FEM simulations demonstrate that the stiffness difference ratio of the anchored diamond coupling TFG is 16.08 times larger than the direct coupling one while the vibration output is reduced by 94.1%. Consequently, the proposed new anchored diamond coupling TFG can structurally increase the stiffness difference ratio to improve the mode ordering and considerably reduce the vibration sensitivity without sacrificing the scale factor.

  10. Preserving the Dnipro River

    International Development Research Centre (IDRC) Digital Library (Canada)

    Humanity inherited the true sense of proportion, synergy, and harmony from the natural environment. ..... In Ukraine, the middle and lower sections of the Dnipro have a drainage ... The following large cities are located in the Dnipro basin: in Russia, .... In Kherson Oblast and in river basins of some small rivers it is as high as ...

  11. Numerical modelling of river processes: flow and river bed deformation

    NARCIS (Netherlands)

    Tassi, P.A.

    2007-01-01

    The morphology of alluvial river channels is a consequence of complex interaction among a number of constituent physical processes, such as flow, sediment transport and river bed deformation. This is, an alluvial river channel is formed from its own sediment. From time to time, alluvial river

  12. Uranium in river water

    International Nuclear Information System (INIS)

    Palmer, M.R.; Edmond, J.M.

    1993-01-01

    The concentration of dissolved uranium has been determined in over 250 river waters from the Orinoco, Amazon, and Ganges basins. Uranium concentrations are largely determined by dissolution of limestones, although weathering of black shales represents an important additional source in some basins. In shield terrains the level of dissolved U is transport limited. Data from the Amazon indicate that floodplains do not represent a significant source of U in river waters. In addition, the authors have determined dissolved U levels in forty rivers from around the world and coupled these data with previous measurements to obtain an estimate for the global flux of dissolved U to the oceans. The average concentration of U in river waters is 1.3 nmol/kg, but this value is biased by very high levels observed in the Ganges-Brahmaputra and Yellow rivers. When these river systems are excluded from the budget, the global average falls to 0.78 nmol/kg. The global riverine U flux lies in the range of 3-6 x 10 7 mol/yr. The major uncertainty that restricts the accuracy of this estimate (and that of all other dissolved riverine fluxes) is the difficulty in obtaining representative samples from rivers which show large seasonal and annual variations in runoff and dissolved load

  13. Umatilla River Basin Anadromous Fish Habitat Enhancement Project : 1995 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, R.Todd

    1996-05-01

    During the 1995 - 96 project period, four new habitat enhancement projects were implemented under the Umatilla River Basin Anadromous Fish Habitat Enhancement Project by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) in the upper Umatilla River Basin. A total of 38,644 feet of high tensile smooth wire fencing was constructed along 3.6 miles of riparian corridor in the Meacham Creek, Wildhorse Creek, Greasewood Creek, West Fork of Greasewood Creek and Mission Creek watersheds. Additional enhancements on Wildhorse Creek and the lower Greasewood Creek System included: (1) installation of 0.43 miles of smooth wire between river mile (RM) 10.25 and RM 10.5 Wildhorse Creek (fence posts and structures had been previously placed on this property during the 1994 - 95 project period), (2) construction of 46 sediment retention structures in stream channels and maintenance to 18 existing sediment retention structures between RM 9.5 and RM 10.25 Wildhorse Creek, and (3) revegetation of stream corridor areas and adjacent terraces with 500 pounds of native grass seed or close species equivalents and 5,000 native riparian shrub/tree species to assist in floodplain recovery, stream channel stability and filtering of sediments during high flow periods. U.S. Fish and Wildlife Service (USFWS), Bureau of Indian Affairs (BIA) and Environmental Protection Agency (EPA) funds were cost shared with Bonneville Power Administration (BPA) funds, provided under this project, to accomplish habitat enhancements. Water quality monitoring continued and was expanded for temperature and turbidity throughout the upper Umatilla River Watershed. Physical habitat surveys were conducted on the lower 13 river miles of Wildhorse Creek and within the Greasewood Creek Project Area to characterize habitat quality and to quantify various habitat types by area.

  14. Savannah River Plant environment

    International Nuclear Information System (INIS)

    Dukes, E.K.

    1984-03-01

    On June 20, 1972, the Atomic Energy Commission designated 192,323 acres of land near Aiken, SC, as the nation's first National Environmental Research Park. The designated land surrounds the Department of Energy's Savannah River Plant production complex. The site, which borders the Savannah River for 17 miles, includes swampland, pine forests, abandoned town sites, a large man-made lake for cooling water impoundment, fields, streams, and watersheds. This report is a description of the geological, hydrological, meteorological, and biological characteristics of the Savannah River Plant site and is intended as a source of information for those interested in environmental research at the site. 165 references, 68 figures, 52 tables

  15. Evaluation of Calendar Year 1996 groundwater and surface water quality data for the Upper East Fork Poplar Creek Hydrogeologic Regime at the US Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1997-09-01

    This report presents an evaluation of the groundwater monitoring data obtained in the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) during calendar year (CY) 1996. The East Fork Regime encompasses several confirmed and suspected sources of groundwater contamination within industrialized areas of the US Department of Energy (DOE) Y-12 Plant in Bear Creek Valley (BCV) southeast of Oak Ridge, Tennessee. The CY 1996 groundwater and surface water monitoring data are presented in Calendar Year 1996 Annual Groundwater Monitoring Report for the Upper East Fork Poplar Creek Hydrogeologic Regime at the US Department of Energy Y-12 Plant, Oak Ridge, Tennessee, along with the required data evaluations specified in the Resource Conservation and Recovery Act (RCRA) post-closure permit for the East Fork Regime. This report provides additional evaluation of the CY 1996 groundwater and surface water monitoring data with an emphasis on regime-wide groundwater contamination and long-term concentration trends for regulated and non-regulated monitoring parameters

  16. Assessing the impacts of river regulation on native bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) habitats in the upper Flathead River, Montana, USA

    Science.gov (United States)

    Muhlfeld, Clint C.; Jones, Leslie A.; Kotter, D.; Miller, William J.; Geise, Doran; Tohtz, Joel; Marotz, Brian

    2012-01-01

    Hungry Horse Dam on the South Fork Flathead River, Montana, USA, has modified the natural flow regimen for power generation, flood risk management and flow augmentation for anadromous fish recovery in the Columbia River. Concern over the detrimental effects of dam operations on native resident fishes prompted research to quantify the impacts of alternative flow management strategies on threatened bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) habitats. Seasonal and life‐stage specific habitat suitability criteria were combined with a two‐dimensional hydrodynamic habitat model to assess discharge effects on usable habitats. Telemetry data used to construct seasonal habitat suitability curves revealed that subadult (fish that emigrated from natal streams to the river system) bull trout move to shallow, low‐velocity shoreline areas at night, which are most sensitive to flow fluctuations. Habitat time series analyses comparing the natural flow regimen (predam, 1929–1952) with five postdam flow management strategies (1953–2008) show that the natural flow conditions optimize the critical bull trout habitats and that the current strategy best resembles the natural flow conditions of all postdam periods. Late summer flow augmentation for anadromous fish recovery, however, produces higher discharges than predam conditions, which reduces the availability of usable habitat during this critical growing season. Our results suggest that past flow management policies that created sporadic streamflow fluctuations were likely detrimental to resident salmonids and that natural flow management strategies will likely improve the chances of protecting key ecosystem processes and help to maintain and restore threatened bull trout and westslope cutthroat trout populations in the upper Columbia River Basin.

  17. The Drosophila FoxA ortholog Fork head regulates growth and gene expression downstream of Target of rapamycin.

    Directory of Open Access Journals (Sweden)

    Margret H Bülow

    2010-12-01

    Full Text Available Forkhead transcription factors of the FoxO subfamily regulate gene expression programs downstream of the insulin signaling network. It is less clear which proteins mediate transcriptional control exerted by Target of rapamycin (TOR signaling, but recent studies in nematodes suggest a role for FoxA transcription factors downstream of TOR. In this study we present evidence that outlines a similar connection in Drosophila, in which the FoxA protein Fork head (FKH regulates cellular and organismal size downstream of TOR. We find that ectopic expression and targeted knockdown of FKH in larval tissues elicits different size phenotypes depending on nutrient state and TOR signaling levels. FKH overexpression has a negative effect on growth under fed conditions, and this phenotype is not further exacerbated by inhibition of TOR via rapamycin feeding. Under conditions of starvation or low TOR signaling levels, knockdown of FKH attenuates the size reduction associated with these conditions. Subcellular localization of endogenous FKH protein is shifted from predominantly cytoplasmic on a high-protein diet to a pronounced nuclear accumulation in animals with reduced levels of TOR or fed with rapamycin. Two putative FKH target genes, CG6770 and cabut, are transcriptionally induced by rapamycin or FKH expression, and silenced by FKH knockdown. Induction of both target genes in heterozygous TOR mutant animals is suppressed by mutations in fkh. Furthermore, TOR signaling levels and FKH impact on transcription of the dFOXO target gene d4E-BP, implying a point of crosstalk with the insulin pathway. In summary, our observations show that an alteration of FKH levels has an effect on cellular and organismal size, and that FKH function is required for the growth inhibition and target gene induction caused by low TOR signaling levels.

  18. The Late Cretaceous Middle Fork caldera, its resurgent intrusion, and enduring landscape stability in east-central Alaska

    Science.gov (United States)

    Bacon, Charles R.; Dusel-Bacon, Cynthia; Aleinikoff, John N.; Slack, John F.

    2014-01-01

    Dissected caldera structures expose thick intracaldera tuff and, uncommonly, cogenetic shallow plutons, while remnants of correlative outflow tuffs deposited on the pre-eruption ground surface record elements of ancient landscapes. The Middle Fork caldera encompasses a 10 km × 20 km area of rhyolite welded tuff and granite porphyry in east-central Alaska, ∼100 km west of the Yukon border. Intracaldera tuff is at least 850 m thick. The K-feldspar megacrystic granite porphyry is exposed over much of a 7 km × 12 km area having 650 m of relief within the western part of the caldera fill. Sensitive high-resolution ion microprobe with reverse geometry (SHRIMP-RG) analyses of zircon from intracaldera tuff, granite porphyry, and outflow tuff yield U-Pb ages of 70.0 ± 1.2, 69.7 ± 1.2, and 71.1 ± 0.5 Ma (95% confidence), respectively. An aeromagnetic survey indicates that the tuff is reversely magnetized, and, therefore, that the caldera-forming eruption occurred in the C31r geomagnetic polarity chron. The tuff and porphyry have arc geochemical signatures and a limited range in SiO2 of 69 to 72 wt%. Although their phenocrysts differ in size and abundance, similar quartz + K-feldspar + plagioclase + biotite mineralogy, whole-rock geochemistry, and analytically indistinguishable ages indicate that the tuff and porphyry were comagmatic. Resorption of phenocrysts in tuff and porphyry suggests that these magmas formed by thermal rejuvenation of near-solidus or solidified crystal mush. A rare magmatic enclave (54% SiO2, arc geochemical signature) in the porphyry may be similar to parental magma and provides evidence of mafic magma and thermal input.

  19. The Western progression of lyme disease: infectious and Nonclonal Borrelia burgdorferi Sensu Lato populations in Grand Forks County, North Dakota.

    Science.gov (United States)

    Stone, Brandee L; Russart, Nathan M; Gaultney, Robert A; Floden, Angela M; Vaughan, Jefferson A; Brissette, Catherine A

    2015-01-01

    Scant attention has been paid to Lyme disease, Borrelia burgdorferi, Ixodes scapularis, or reservoirs in eastern North Dakota despite the fact that it borders high-risk counties in Minnesota. Recent reports of B. burgdorferi and I. scapularis in North Dakota, however, prompted a more detailed examination. Spirochetes cultured from the hearts of five rodents trapped in Grand Forks County, ND, were identified as B. burgdorferi sensu lato through sequence analyses of the 16S rRNA gene, the 16S rRNA gene-ileT intergenic spacer region, flaB, ospA, ospC, and p66. OspC typing revealed the presence of groups A, B, E, F, L, and I. Two rodents were concurrently carrying multiple OspC types. Multilocus sequence typing suggested the eastern North Dakota strains are most closely related to those found in neighboring regions of the upper Midwest and Canada. BALB/c mice were infected with B. burgdorferi isolate M3 (OspC group B) by needle inoculation or tick bite. Tibiotarsal joints and ear pinnae were culture positive, and B. burgdorferi M3 was detected by quantitative PCR (qPCR) in the tibiotarsal joints, hearts, and ear pinnae of infected mice. Uninfected larval I. scapularis ticks were able to acquire B. burgdorferi M3 from infected mice; M3 was maintained in I. scapularis during the molt from larva to nymph; and further, M3 was transmitted from infected I. scapularis nymphs to naive mice, as evidenced by cultures and qPCR analyses. These results demonstrate that isolate M3 is capable of disseminated infection by both artificial and natural routes of infection. This study confirms the presence of unique (nonclonal) and infectious B. burgdorferi populations in eastern North Dakota. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  20. Farm to Fork Quantitative Risk Assessment of Listeria monocytogenes Contamination in Raw and Pasteurized Milk Cheese in Ireland.

    Science.gov (United States)

    Tiwari, Uma; Cummins, Enda; Valero, Antonio; Walsh, Des; Dalmasso, Marion; Jordan, Kieran; Duffy, Geraldine

    2015-06-01

    The objective of this study was to model and quantify the level of Listeria monocytogenes in raw milk cheese (RMc) and pasteurized milk cheese (PMc) from farm to fork using a Bayesian inference approach combined with a quantitative risk assessment. The modeling approach included a prediction of contamination arising from the farm environment as well from cross-contamination within the cheese-processing facility through storage and subsequent human exposure. The model predicted a high concentration of L. monocytogenes in contaminated RMc (mean 2.19 log10 CFU/g) compared to PMc (mean -1.73 log10 CFU/g). The mean probability of illness (P1 for low-risk population, LR) and (P2 for high-risk population, HR, e.g., immunocompromised) adult Irish consumers following exposure to contaminated cheese was 7 × 10(-8) (P1 ) and 9 × 10(-4) (P2 ) for RMc and 7 × 10(-10) (P1 ) and 8 × 10(-6) (P2 ) for PMc, respectively. In addition, the model was used to evaluate performance objectives at various stages, namely, the cheese making and ripening stages, and to set a food safety objective at the time of consumption. A scenario analysis predicted various probabilities of L. monocytogenes contamination along the cheese-processing chain for both RMc and PMc. The sensitivity analysis showed the critical factors for both cheeses were the serving size of the cheese, storage time, and temperature at the distribution stage. The developed model will allow food processors and policymakers to identify the possible routes of contamination along the cheese-processing chain and to reduce the risk posed to human health. © 2015 Society for Risk Analysis.

  1. Hg isotopes reveal in-stream processing and legacy inputs in East Fork Poplar Creek, Oak Ridge, Tennessee, USA.

    Science.gov (United States)

    Demers, Jason D; Blum, Joel D; Brooks, Scott C; Donovan, Patrick M; Riscassi, Ami L; Miller, Carrie L; Zheng, Wang; Gu, Baohua

    2018-04-25

    Natural abundance stable Hg isotope measurements were used to place new constraints on sources, transport, and transformations of Hg along the flow path of East Fork Poplar Creek (EFPC), a point-source contaminated headwater stream in Oak Ridge, Tennessee. Particulate-bound Hg in the water column of EFPC within the Y-12 National Security Complex, was isotopically similar to average metallic Hg(0) used in industry, having a mean δ202Hg value of -0.42 ± 0.09‰ (1SD) and near-zero Δ199Hg. On average, particulate fraction δ202Hg values increased downstream by 0.53‰, while Δ199Hg decreased by -0.10‰, converging with the Hg isotopic composition of the fine fraction of streambed sediment along the 26 km flow path. The dissolved fraction behaved differently. Although initial Δ199Hg values of the dissolved fraction were also near-zero, these values increased transiently along the flow path. Initial δ202Hg values of the dissolved fraction were more variable than in the particulate fraction, ranging from -0.44 to 0.18‰ among three seasonal sampling campaigns, but converged to an average δ202Hg value of 0.01 ± 0.10‰ (1SD) downstream. Dissolved Hg in the hyporheic and riparian pore water had higher and lower δ202Hg values, respectively, compared to dissolved Hg in stream water. Variations in Hg isotopic composition of the dissolved and suspended fractions along the flow path suggest that: (1) physical processes such as dilution and sedimentation do not fully explain decreases in total mercury concentrations along the flow path; (2) in-stream processes include photochemical reduction, but microbial reduction is likely more dominant; and (3) additional sources of dissolved mercury inputs to EFPC at baseflow during this study predominantly arise from the hyporheic zone.

  2. Second report on the Oak Ridge Y-12 Plant fish kill for Upper East Fork Poplar Creek

    International Nuclear Information System (INIS)

    Etnier, E.L.; Opresko, D.M.; Talmage, S.S.

    1994-08-01

    This report summarizes the monitoring of fish kills in upper East Fork Poplar Creek (EFPC) from July 1990 to June 1993. Since the opening of Lake Reality (LR) in 1988, total numbers of fish inhabiting upper EFPC have increased. However, species diversity has remained poor. Water quality data have been collected in upper EFPC during the time period covered in this report. Total residual chlorine (TRC) levels have exceeded federal and state water quality criteria over the years. However, with the installation of two dechlorination systems in late 1992, TRC levels have been substantially lowered in most portions of upper EFPC. By June 1993, concentrations of TRC were 0.04 to 0.06 mg/L at the north-south pipes (NSP) and below detection limits at sampling station AS-8 and were 0 to 0.01 mg/L at the inlet and outlet of LR. The daily chronic fish mortality in upper EFPC has been attributed to background stress resulting from the continuous discharge of chlorine into upper EFPC. Mean daily mortality rates for 22 acute fish kills were three fold or more above background and usually exceeded ten fish per day. Total number of dead fish collected per acute kill event ranged from 30 to over 1,000 fish; predominant species killed were central stonerollers (Campostoma anomalum) and striped shiners (Luxilus chrysocephalus). Spills or elevated releases of toxic chemicals, such as acids, organophosphates, aluminum nitrate, ammonia, or chlorine, were identified as possible causative agents; however, a definitive cause-effect relationship was rarely established for any acute kills. Ambient toxicity testing, in situ chemical monitoring, and streamside experiments were used to examine TRC dynamics and ambient toxicity in EFPC

  3. Second report on the Oak Ridge Y-12 Plant fish kill for Upper East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Etnier, E.L.; Opresko, D.M.; Talmage, S.S. [eds.

    1994-08-01

    This report summarizes the monitoring of fish kills in upper East Fork Poplar Creek (EFPC) from July 1990 to June 1993. Since the opening of Lake Reality (LR) in 1988, total numbers of fish inhabiting upper EFPC have increased. However, species diversity has remained poor. Water quality data have been collected in upper EFPC during the time period covered in this report. Total residual chlorine (TRC) levels have exceeded federal and state water quality criteria over the years. However, with the installation of two dechlorination systems in late 1992, TRC levels have been substantially lowered in most portions of upper EFPC. By June 1993, concentrations of TRC were 0.04 to 0.06 mg/L at the north-south pipes (NSP) and below detection limits at sampling station AS-8 and were 0 to 0.01 mg/L at the inlet and outlet of LR. The daily chronic fish mortality in upper EFPC has been attributed to background stress resulting from the continuous discharge of chlorine into upper EFPC. Mean daily mortality rates for 22 acute fish kills were three fold or more above background and usually exceeded ten fish per day. Total number of dead fish collected per acute kill event ranged from 30 to over 1,000 fish; predominant species killed were central stonerollers (Campostoma anomalum) and striped shiners (Luxilus chrysocephalus). Spills or elevated releases of toxic chemicals, such as acids, organophosphates, aluminum nitrate, ammonia, or chlorine, were identified as possible causative agents; however, a definitive cause-effect relationship was rarely established for any acute kills. Ambient toxicity testing, in situ chemical monitoring, and streamside experiments were used to examine TRC dynamics and ambient toxicity in EFPC.

  4. High quality-factor quartz tuning fork glass probe used in tapping mode atomic force microscopy for surface profile measurement

    Science.gov (United States)

    Chen, Yuan-Liu; Xu, Yanhao; Shimizu, Yuki; Matsukuma, Hiraku; Gao, Wei

    2018-06-01

    This paper presents a high quality-factor (Q-factor) quartz tuning fork (QTF) with a glass probe attached, used in frequency modulation tapping mode atomic force microscopy (AFM) for the surface profile metrology of micro and nanostructures. Unlike conventionally used QTFs, which have tungsten or platinum probes for tapping mode AFM, and suffer from a low Q-factor influenced by the relatively large mass of the probe, the glass probe, which has a lower density, increases the Q-factor of the QTF probe unit allowing it to obtain better measurement sensitivity. In addition, the process of attaching the probe to the QTF with epoxy resin, which is necessary for tapping mode AFM, is also optimized to further improve the Q-factor of the QTF glass probe. The Q-factor of the optimized QTF glass probe unit is demonstrated to be very close to that of a bare QTF without a probe attached. To verify the effectiveness and the advantages of the optimized QTF glass probe unit, the probe unit is integrated into a home-built tapping mode AFM for conducting surface profile measurements of micro and nanostructures. A blazed grating with fine tool marks of 100 nm, a microprism sheet with a vertical amplitude of 25 µm and a Fresnel lens with a steep slope of 90 degrees are used as measurement specimens. From the measurement results, it is demonstrated that the optimized QTF glass probe unit can achieve higher sensitivity as well as better stability than conventional probes in the measurement of micro and nanostructures.

  5. Use of a watershed-modeling approach to assess hydrologic effects of urbanization, North Fork Pheasant Branch basin near Middleton, Wisconsin

    Science.gov (United States)

    Steuer, Jeffrey J.; Hunt, R.J.

    2001-01-01

    The North Fork Pheasant Branch Basin in Dane County, Wisconsin is expected to undergo development. There are concerns that development will adversely affect water resources with increased flood peaks, increased runoff volumes, and increased pollutant loads. To provide a scientific basis for evaluating the hydrologic system response to development the Precipitation Runoff Modeling System (PRMS) was used to model the upper Pheasant Branch Creek watershed with an emphasis on the North Fork Basin. The upper Pheasant Branch Creek (18.3 mi2; 11,700 acres) Basin was represented with 21 Hydrologic Response Units (daily time step) and 50 flow planes (5-minute time steps). Precipitation data from the basin outlet streamflow-gaging station located at Highway 12 and temperature data from a nearby airport were used to drive the model. Continuous discharge records at three gaging stations were used for model calibration. To qualitatively assess model representation of small subbasins, periodic reconnaissance, often including a depth measurement, was made after precipitation to determine the occurrence of flow in ditches and channels from small subbasins. As a further effort to verify the model on a small subbasin scale, continuous-stage sensors (15-minute intervals) measured depth at the outlets of three small subbasins (500 to 1,200 acres). Average annual precipitation for the simulation period from 1993 to 1998 was 35.2 inches. The model simulations showed that, on average, 23.9 inches were intercepted by vegetation, or lost to evapotranspiration, 6.0 inches were infiltrated and moved to the regional ground-water system, and 4.8 inches contributed to the upper Pheasant Branch streamflow. The largest runoff event during the calibration interval was in July 1993 (746 ft3/sec; with a recurrence interval of approximately 25 years). Resulting recharge rates from the calibrated model were subsequently used as input into a ground-water-flow model. Average annual recharge varied

  6. Hunting camp. River Murray

    OpenAIRE

    ? Bayliss, Charles, 1850-1897, photographer

    2003-01-01

    200 x 149 mm. A good photograph showing a group of aborigines (in European clothes) with two hunting dogs, holding spears and standing in front of rough wooden cabins; with the river in the background. Photograph unknown, possible Charles Bayliss.

  7. Wild and Scenic Rivers

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This map layer portrays the linear federally-owned land features (i.e., national parkways, wild and scenic rivers, etc.) of the United States, Puerto Rico, and the...

  8. Concentration-Discharge relationships in a mine-impacted catchment, New River, Tennessee: Comparison across spatial and temporal scales using time-series analysis

    Science.gov (United States)

    Murphy, J.; Hornberger, G. M.

    2009-12-01

    Concentration-discharge (c-Q) relationships are useful in indentifying physical and chemical processes affecting stream water chemistry. Frequently used as a diagnostic tool, c-Q relationships can be used to infer particular mixing patterns that may occur in a catchment. However, much work has shown c-Q relationships are highly variable and often inconclusive, suggesting the catchment behavior they indicate cannot be readily recognized without supporting knowledge of system dynamics. For example, drainage area and location, in addition to changes in land use over time, affect many processes in catchments including flow routing and solute concentrations. The effect of spatial and temporal scales on c-Q relationships are explored using recursive time-series analysis of historic and recent water quality data. The New River encompasses 400 square miles of remote land on the Cumberland Plateau in middle Tennessee and is a major component of the headwaters of the Cumberland River. Current and historic coal mining, oil and gas extraction, and timber harvesting have impaired water quality in the watershed. Historically, the highest magnitude of degradation probably occurred during the mid-1900s with severe acid mine drainage throughout the watershed. In 1975, 56% of all coal mined in Tennessee was derived from the New River watershed. Over the past three decades most of the New River system has rebounded though some small tributaries still experience acid mine drainage and elevated metal loads. Sediment, in terms of quantity and sorption of metals, is currently considered the largest pollutant by many. Water pH is circum-neutral in the system and coal-mining pollution is best identified by elevated specific conductance and sulfate concentration. A combination of historic and recently collected water quality data were obtained for the Indian Fork, a small 4 square mile upland catchment in the New River watershed, and the New River main-stem, approximately 25 miles

  9. [Immunoprecipitation mapping of the TRX-associated chromosome elements in the fork head gene promoter in the Drosophila melanogaster salivary gland cells].

    Science.gov (United States)

    Riakhovskiĭ, A A; Tillib, S V

    2007-09-01

    Using the method of immunoprecipitation of the in vivo crosslinked and sheared by sonication chromatin, mapping of potential trithorax-associated regulatory elements within the extended (9 kb) promoter region of the fork head gene (fkh) in the Drosophila melanogaster salivary gland cells was performed. Relative homogeneity of the salivary gland cells, along with the parallel use of the antibodies to different domains of the same trithorax protein (TRX), and the introduction of cross-hybridization steps for additional specific enrichment of initial DNA libraries, provided improvement of the method effectiveness and identification of one major and two less expressed potential TRX-binding sites.

  10. Developing a Natural Gas-Powered Bus Rapid Transit Service: A Case Study on Leadership: Roaring Fork Transportation Authority (Presentation); NREL (National Renewable Energy Laboratory)

    Energy Technology Data Exchange (ETDEWEB)

    Schroeder, A.

    2015-03-01

    The Roaring Fork Transportation Authority (RFTA) represents a series of unique successes in alternative fuel deployment by pushing the envelope with innovative solutions. In the last year, RFTA demonstrated the ability to utilize compressed natural gas buses at a range of altitudes, across long distances, in extreme weather conditions and in a modern indoor fueling and maintenance facility - allwhile saving money and providing high-quality customer service. This case study will highlight how the leadership of organizations and communities that are implementing advances in natural gas vehicle technology is paving the way for broader participation.

  11. Assessment of water and proppant quantities associated with petroleum production from the Bakken and Three Forks Formations, Williston Basin Province, Montana and North Dakota, 2016

    Science.gov (United States)

    Haines, Seth; Varela, Brian A.; Hawkins, Sarah J.; Gianoutsos, Nicholas J.; Thamke, Joanna N.; Engle, Mark A.; Tennyson, Marilyn E.; Schenk, Christopher J.; Gaswirth, Stephanie B.; Marra, Kristen R.; Kinney, Scott A.; Mercier, Tracey J.; Martinez, Cericia D.

    2017-06-23

    The U.S. Geological Survey (USGS) has completed an assessment of water and proppant requirements and water production associated with the possible future production of undiscovered oil and gas resources in the Three Forks and Bakken Formations (Late Devonian to Early Mississippian) of the Williston Basin Province in Montana and North Dakota. This water and proppant assessment is directly linked to the geology-based assessment of the undiscovered, technically recoverable continuous oil and gas resources that is described in USGS Fact Sheet 2013–3013.

  12. Toutle/Cowlitz River Sediment Budget

    Science.gov (United States)

    2010-05-18

    the stream width, expressed as Watts per square meter (W/m2). Brookes (1987) found that in streams 25 30 35 40 45 50 Jun-68 Dec-73 May-79 Nov-84...0.0100 0.1000 0.1 1.0 10.0 100.0 En er gy S lo pe (m /m ) Bankfull Discharge per Unit Width, (cubic meters /sec-m) North Fork Above SRS North Fork Below...at 5 feet per second, however, Gray et al. (2009) report that the FISP series of isokinetic samplers is calibrated to 3.9 feet per second. As shown

  13. Calendar year 1995 groundwater quality report for the upper east Fork Poplar Creek Hydrogeologic regime, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. 1995 Groundwater quality data and calculated rate of contaminant migration

    International Nuclear Information System (INIS)

    1996-02-01

    This annual groundwater quality report (GWQR) contains groundwater and surface water quality data obtained during the 1995 calendar year (CY) at several waste management facilities associated with the U.S. Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee. The U.S. Environmental Protection Agency (EPA) identification number for the Y-12 Plant is TN3 89 009 0001. The sites addressed by this document are located within the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime). The East Fork Regime, which is one of three hydrogeologic regimes defined for the purposes of groundwater quality monitoring at the Y-12 Plant, encompasses the Y-12 Plant

  14. Calendar Year 1997 Annual Groundwater Monitoring Report For The Upper East Fork Poplar Creek Hydrogeologic Regime At The U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Jones, S.B.

    1998-02-01

    This report contains the groundwater monitoring data obtained during calendar year (CY) 1997 in compliance with the Resource Conservation Wd Recovery Act (RCRA) post-closure permit (PCP) for the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) at the U.S. Department of Energy (DOE) Y-12 Plant in Oak Ridge, Tennessee. Issued by the Tennessee Department of Environment and Conservation (TDEC), the PCP defines the RCRA post-closure corrective action monitoring requirements for the portion of the groundwater contaminant plume that has migrated into the East Fork Regime ftom the S-3 Ponds, a closed RCW-regulated former surface impoundment located in Bear Creek Valley near the west end of the Y-12 Plant. In addition to the RCIL4 post-closure corrective action monitoring results, this report contains the groundwater and surface water monitoring data obtained during CY 1997 to fulfill requirements of DOE Order 5400.1.

  15. Effects of the Operation of Kerr and Hungry Horse Dams on the Kokanee Fishery in the Flathead River System, 1979-1985 Final Research Report.

    Energy Technology Data Exchange (ETDEWEB)

    Clancy, Patrick

    1986-05-01

    This study was undertaken to assess the effects of the operation of Hungry Horse Dam on the kokanee fishery in the Flathead River system. Studies concerning operation of the dam on the Flathead River aquatic biota began in 1979 and continued to 1982 under Bureau of Reclamation funding. These studies resulted in flow recommendations for the aquatic biota in the main stem Flathead River, below the influence of Hungry Horse Dam on the South Fork. Studies concerned specifically with kokanee salmon have continued under Bonneville Power Administration funding since 1982. This completion report covers the entire study period (September 1979 to June 1985). Major results of this study were: (1) development and refinement of methods to assess hydropower impacts on spawning and incubation success of kokanee; (2) development of a model to predict kokanee year class strength from Flathead River flows; and (3) implementation of flows favorable for successful kokanee reproduction. A monitoring program has been developed which will assess the recovery of the kokanee population as it proceeds, and to recommend management strategies to maintain management goals for the kokanee fishery in the river system.

  16. [Health assessment of river ecosystem in Haihe River Basin, China].

    Science.gov (United States)

    Hao, Li-Xia; Sun, Ran-Hao; Chen, Li-Ding

    2014-10-01

    With the development of economy, the health of river ecosystem is severely threatened because of the increasing effects of human activities on river ecosystem. In this paper, the authors assessed the river ecosystem health in aspects of chemical integrity and biological integrity, using the criterion in water quality, nutrient, and benthic macroinvertebrates of 73 samples in Haihe River Basin. The research showed that the health condition of river ecosystem in Haihe River Basin was bad overall since the health situation of 72. 6% of the samples was "extremely bad". At the same time, the health situation in Haihe River Basin exhibited obvious regional gathering effect. We also found that the river water quality was closely related to human activities, and the eutrophication trend of water body was evident in Haihe River Basin. The biodiversity of the benthic animal was low and lack of clean species in the basin. The indicators such as ammonia nitrogen, total nitrogen and total phosphorus were the key factors that affected the river ecosystem health in Haihe River Basin, so the government should start to curb the deterioration of river ecosystem health by controlling these nutrients indicators. For river ecosystem health assessment, the multi-factors comprehensive evaluation method was superior to single-factor method.

  17. Hydrologic data summary for the St. Lucie River Estuary, Martin and St. Lucie Counties, Florida, 1998-2001

    Science.gov (United States)

    Byrne, Michael J.; Patino, Eduardo

    2004-01-01

    A hydrologic analysis was made at three canal sites and four tidal sites along the St. Lucie River Estuary in southeastern Florida from 1998 to 2001. The data included for analysis are stage, 15-minute flow, salinity, water temperature, turbidity, and suspended-solids concentration. During the period of record, the estuary experienced a drought, major storm events, and high-water discharge from Lake Okeechobee. Flow mainly occurred through the South Fork of the St. Lucie River; however, when flow increased through control structures along the C-23 and C-24 Canals, the North Fork was a larger than usual contributor of total freshwater inflow to the estuary. At one tidal site (Steele Point), the majority of flow was southward toward the St. Lucie Inlet; at a second tidal site (Indian River Bridge), the majority of flow was northward into the Indian River Lagoon. Large-volume stormwater discharge events greatly affected the St. Lucie River Estuary. Increased discharge typically was accompanied by salinity decreases that resulted in water becoming and remaining fresh throughout the estuary until the discharge events ended. Salinity in the estuary usually returned to prestorm levels within a few days after the events. Turbidity decreased and salinity began to increase almost immediately when the gates at the control structures closed. Salinity ranged from less than 1 to greater than 35 parts per thousand during the period of record (1998-2001), and typically varied by several parts per thousand during a tidal cycle. Suspended-solids concentrations were observed at one canal site (S-80) and two tidal sites (Speedy Point and Steele Point) during a discharge event in April and May 2000. Results suggest that most deposition of suspended-solids concentration occurs between S-80 and Speedy Point. The turbidity data collected also support this interpretation. The ratio of inorganic to organic suspended-solids concentration observed at S-80, Speedy Point, and Steele Point

  18. Current mercury distribution and bioavailability in floodplain soils of Lower East Fork Poplar Creek, Oak Ridge, Tennessee, USA

    International Nuclear Information System (INIS)

    Han, Fengxiang X.; Su, Yi; Monts, David L.

    2011-01-01

    The objectives of this study were to investigate the current status of mercury distribution, speciation and bioavailability in the floodplain soils of Lower East Fork Poplar Creek (LEFPC) after decades of US Department of Energy's remediation. Historically as part of its national security mission, the U.S. Department of Energy's Y-12 National Security Facility in Oak Ridge, TN, USA acquired a significant fraction of the world's supply of elemental mercury. During the 1950s and 1960s, a large amount of elemental mercury escaped confinement and is still present in the watershed surrounding the Y-12 facility. A series of remediation efforts have been deployed in the watersheds around the Oak Ridge site during the following years. The sampling fields were located in a floodplain of LEFPC of Oak Ridge, TN, USA. A series of surface soils (10-20 cm) were sampled from both wooded areas and wetland/grass land. Two 8x8 m fields were selected in the woodland. Five profiles each consisting of three layers were randomly taken from each field. The three layers were the surface layer at 0-10cm, subsurface layer at 50-60 cm, and bottom layer at 100-110 cm. Soil in both wood and wetland areas was well developed with a clear B horizon. The present study clearly shows that the total mercury in floodplain soils of LEFPC significantly decreased after the series of remediation. This study confirmed the long-term effectiveness of these remediation actions, especially after excavation of highly contaminated floodplain soils. However, the average total mercury level of all soil samples collected are in the range of 50-80 mg/kg, still significantly above toxic level (> 5mg/kg). Furthermore, contrary to conventional believing, the major mercury form in current soils of this particular area of floodplain of LEFPC is mainly in non-cinnabar mercury bound in clay minerals (after decades of remediation). The floodplains can act both as a medium-term sink and as long-term sources. Native North

  19. Sources of acid and metals from the weathering of the Dinero waste pile, Lake Fork watershed, Leadville, Colorado

    Science.gov (United States)

    Diehl, S.F.; Hageman, Phil L.; Smith, Kathleen S.; Herron, J.T.; Desborough, G.A.

    2005-01-01

    Two trenches were dug into the south Dinero mine-waste pile near Leadville, Colorado, to study the weathering of rock fragments and the mineralogic sources of metal contaminants in the surrounding wetland and Lake Fork Watershed. Water seeping from the base of the south Dinero waste-rock pile was pH 2.9, whereas leachate from a composite sample of the rock waste was pH 3.3. The waste pile was mostly devoid of vegetation, open to infiltration of precipitation, and saturated at the base because of placement in the wetland. The south mine-waste pile is composed of poorly sorted material, ranging from boulder-size to fine-grained rock fragments. The trenches showed both matrix-supported and clast-supported zones, with faint horizontal color banding, suggesting zonation of Fe oxides. Secondary minerals such as jarosite and gypsum occurred throughout the depth of the trenches. Infiltration of water and transport of dissolved material through the pile is evidenced by optically continuous secondary mineral deposits that fill or line voids. Iron-sulfate material exhibits microlaminations with shrinkage cracking and preferential dissolution of microlayers that evidence drying and wetting events. In addition to fluids, submicron-sized to very fine-grained particles such as jarosite are transported through channel ways in the pile. Rock fragments are coated with a mixture of clay, jarosite, and manganese oxides. Dissolution of minerals is a primary source of metals. Skeletal remnants of grains, outlined by Fe-oxide minerals, are common. Potassium jarosite is the most abundant jarosite phase, but Pb-and Ag-bearing jarosite are common. Grain-sized clusters of jarosite suggest that entire sulfide grains were replaced by very fine-grained jarosite crystals. The waste piles were removed from the wetland and reclaimed upslope in 2003. This was an opportunity to test methods to identify sources of acid and metals and metal transport processes within a waste pile. A series of

  20. Quantitative farm-to-fork human norovirus exposure assessment of individually quick frozen raspberries and raspberry puree.

    Science.gov (United States)

    Jacxsens, L; Stals, A; De Keuckelaere, A; Deliens, B; Rajkovic, A; Uyttendaele, M

    2017-02-02

    package containing approximately 115 berries. This farm-to-fork model is a useful tool for evaluating NoV mitigation strategies in the soft red fruit supply chain. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. A retrospective study of the chemical analysis cost for the remediation of Lower East Fork Poplar Creek, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Klatt, L.N.

    1998-06-01

    A retrospective study of the remediation of Lower East Fork Poplar Creek (LEFPC) in Oak Ridge, Tennessee was completed. The study was conducted by reviewing the public Comprehensive Environmental Response, Compensation, and Liability Act record documents associated with the remediation of LEFPC and through discussions with the project staff involved or familiar with the project. The remediation took place in two phases. The first phase involved the excavation of about 5,560 yd 3 of soil at the National Oceanic and Atmospheric Administration (NOAA) locations in 1996. The second phase involved the excavation of 39,200 yd 3 at another NOAA location and at the Bruner location in 1997. For the entire project (remedial investigation through cleanup), a total of 7,708 samples (1 sample for each 5.8 yd 3 of soil remediated) were analyzed for mercury. The project obtained special regulatory approval to use two methods for the determination of mercury in soils that are not part of the Resource Conservation and Recovery Act SW-846 methods manual. The mercury analysis cost was $678,000, which represents 9.6% of the cleanup cost. During the cleanup phase of the project, an on-site laboratory was used. The estimated cost savings that the on-site laboratory provided fall into two categories: direct reduction of costs associated with chemical analysis and sample shipment totaling approximately $38,000, which represents a 5.3% savings relative to the estimated cost of using an off-site laboratory, and savings in the amount of $890,000 (12.5% of the $7.1 M cleanup cost), associated with expediting execution of the cleanup work by providing rapid (< 3 hours) sample result turnaround time. The manner in which the analytical services were procured for the LEFPC project suggest that the development of new chemical analysis technology must address deployment, performance, regulatory, robustness, reliability, and business appropriateness factors if the technology is to be used in

  2. Epidemiology and Control of Infectious Diseases of Salmonids in the Columbia River Basin, 1986 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Fryer, John L.

    1986-12-01

    The Department of Microbiology at Oregon State University with funding from the Bonneville Power Administration conducted a study relating to the epidemiology and control of three fish diseases of salmonids in the Columbia River Basin. These three diseases were ceratomyxosis caused by the myxosporidan parasite Ceratomyxa Shasta, bacterial kidney disease, the causative agent Renibacterium salmoninarum, and infectious hematopoietic necrosis, caused by a rhabdovirus. Each of these diseases is highly destructive and difficult or impossible to treat with antimicrobial agents. The geographic range of the infectious stage of C. Shasta has been extended to include the Snake River to the Oxbow and Hells Canyon Dams. These are the farthest upriver sites tested. Infections of ceratomyxosis were also initiated in the east fork of the Lewis River and in the Washougal River in Washington. Laboratory studies with this parasite failed to indicate that tubeficids are required in its life cycle. Bacterial kidney disease has been demonstrated in all life stages of salmonids: in the eggs, fry, smolts, juveniles and adults in the ocean, and in fish returning to fresh water. Monoclonal antibodies produced against R. salmoninarum demonstrated antigenic differences among isolates of the bacterium. Monoclonal antibodies also showed antigens of R. salmoninarum which are similar to those of a wide variety of gram positive and gram negative bacteria. A demonstration project at Round Butte Hatchery showed U V treatment to be an effective method for reducing the microbial population of the water supply and could reduce risks of IHNV. Tangential flow filtration was used successfully to concentrate IHNV from environmental water. At Round Butte Hatchery the carrier rate of IHNV in adults was very low and there was no subsequent mortality resulting from IHN in juveniles.

  3. Growth and smolting in lower-mode Atlantic Salmon stocked into the Penobscot River, Maine

    Science.gov (United States)

    Zydlewski, Joseph D.; O'Malley, Andrew; Cox, Oliver; Ruksznis, Peter; Trial, Joan G.

    2014-01-01

    Restoration of Atlantic Salmon Salmo salar in Maine has relied on hatchery-produced fry and smolts for critical stocking strategies. Stocking fry minimizes domestication selection, but these fish have poor survival. Conversely, stocked smolts have little freshwater experience but provide higher adult returns. Lower-mode (LM) fish, those not growing fast enough to ensure smolting by the time of stocking, are a by-product of the smolt program and are an intermediate hatchery product. From 2002 to 2009, between 70,000 and 170,000 marked LM Atlantic Salmon were stocked into the Pleasant River (a tributary in the Penobscot River drainage, Maine) in late September to early October. These fish were recaptured as actively migrating smolts (screw trapping), as nonmigrants (electrofishing), and as returning adults to the Penobscot River (Veazie Dam trap). Fork length (FL) was measured and a scale sample was taken to retrospectively estimate FL at winter annulus one (FW1) using the intercept-corrected direct proportion model. The LM fish were observed to migrate as age-1, age-2, and infrequently as age-3 smolts. Those migrating as age-1 smolts had a distinctly larger estimated FL at FW1 (>112 mm) than those that remained in the river for at least one additional year. At the time of migration, age-2 and age-3 smolts were substantially larger than age-1 smolts. Returning adult Atlantic Salmon of LM origin had estimated FLs at FW1 that corresponded to smolt age (greater FL for age 1 than age 2). The LM product produces both age-1 and age-2 smolts that have greater freshwater experience than hatchery smolts and may have growth and fitness advantages. The data from this study will allow managers to better assess the probability of smolting age and manipulate hatchery growth rates to produce a targeted-size LM product.

  4. Development of a quartz tuning-fork-based force sensor for measurements in the tens of nanoNewton force range during nanomanipulation experiments

    Energy Technology Data Exchange (ETDEWEB)

    Oiko, V. T. A., E-mail: oiko@ifi.unicamp.br; Rodrigues, V.; Ugarte, D. [Instituto de Física “Gleb Wataghin,” Univ. Estadual de Campinas (UNICAMP), Campinas 13083-859 (Brazil); Martins, B. V. C. [Department of Physics, University of Alberta, Edmonton, Alberta T6G 2R3 (Canada); Silva, P. C. [Laboratório Nacional de Nanotecnologia, CNPEM, Campinas 13083-970 (Brazil)

    2014-03-15

    Understanding the mechanical properties of nanoscale systems requires new experimental and theoretical tools. In particular, force sensors compatible with nanomechanical testing experiments and with sensitivity in the nN range are required. Here, we report the development and testing of a tuning-fork-based force sensor for in situ nanomanipulation experiments inside a scanning electron microscope. The sensor uses a very simple design for the electronics and it allows the direct and quantitative force measurement in the 1–100 nN force range. The sensor response is initially calibrated against a nN range force standard, as, for example, a calibrated Atomic Force Microscopy cantilever; subsequently, applied force values can be directly derived using only the electric signals generated by the tuning fork. Using a homemade nanomanipulator, the quantitative force sensor has been used to analyze the mechanical deformation of multi-walled carbon nanotube bundles, where we analyzed forces in the 5–40 nN range, measured with an error bar of a few nN.

  5. Flood-inundation maps for South Fork Peachtree Creek from the Brockett Road bridge to the Willivee Drive bridge, DeKalb County, Georgia

    Science.gov (United States)

    Musser, Jonathan W.

    2015-10-14

    Digital flood-inundation maps for a 5.3-mile reach of South Fork Peachtree Creek that extends from about 500 feet above the Brockett Road bridge to the Willivee Drive bridge were developed by the U.S. Geological Survey (USGS) in cooperation with DeKalb County, Georgia. The flood-inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage at South Fork Peachtree at Casa Drive, near Clarkston, Georgia (02336152). Real-time stage information from this USGS streamgage may be obtained at http://waterdata.usgs.gov/ and can be used in conjunction with these maps to estimate near real-time areas of inundation. The National Weather Service (NWS) is incorporating results from this study into the Advanced Hydrologic Prediction Service (AHPS) flood-warning system (http://water.weather.gov/ahps/).

  6. Calendar year 1994 groundwater quality report for the Upper East Fork Poplar Creek Hydrogeologic Regime, Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-10-01

    This groundwater quality report (GWQR) contains an evaluation of the groundwater quality data obtained during the 1994 calendar year (CY) at several waste-management facilities and a petroleum fuel underground storage tank (UST) site at the US Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee. These sites lie within the boundaries of the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), which is one of three hydrogeologic regimes defined for the purposes of groundwater quality monitoring at the Y-12 Plant. The Environmental Management Department of the Y-12 Plant Health, Safety, Environment, and Accountability (HSEA) Organization manages the groundwater monitoring activities in each regime under the auspices of the Y-12 Plant Groundwater Protection Program (GWPP). The purpose of the GWPP is to characterize the hydrogeology and to monitor groundwater quality at the Y-12 Plant and surrounding area to ensure protection of local groundwater resources in accordance with federal, state, and local regulations, DOE Orders, and Lockheed Martin Energy Systems, Inc. (Energy Systems) corporate policy

  7. Salmonid Gamete Preservation in the Snake River Basin : 2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Armstrong, Robyn; Kucera, Paul A. [Nez Perce Tribe. Dept. of Fisheries Resource Management, Lapwai, ID (US)

    2001-06-01

    Steelhead (Oncorhynchus mykiss) and chinook salmon (Oncorhynchus tshawytscha) populations in the Northwest are decreasing. Genetic diversity is being lost at an alarming rate. The Nez Perce Tribe (Tribe) strives to ensure availability of genetic samples of the existing male salmonid population by establishing and maintaining a germplasm repository. The sampling strategy, initiated in 1992, has been to collect and preserve male salmon and steelhead genetic diversity across the geographic landscape by sampling within the major river subbasins in the Snake River basin, assuming a metapopulation structure existed historically. Gamete cryopreservation conserves genetic diversity in a germplasm repository, but is not a recovery action for listed fish species. The Tribe was funded in 2000 by the Bonneville Power Administration (BPA) and the U.S. Fish and Wildlife Service Lower Snake River Compensation Plan (LSRCP) to coordinate gene banking of male gametes from Endangered Species Act listed steelhead and spring and summer chinook salmon in the Snake River basin. In 2000, a total of 349 viable chinook salmon semen samples from the Lostine River, Catherine Creek, upper Grande Ronde River, Lookingglass Hatchery (Imnaha River stock), Rapid River Hatchery, Lake Creek, the South Fork Salmon River weir, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi Hatchery, and Sawtooth Hatchery (upper Salmon River stock) were cryopreserved. Also, 283 samples of male steelhead gametes from Dworshak Hatchery, Fish Creek, Grande Ronde River, Imnaha River, Little Sheep Creek, Pahsimeroi Hatchery and Oxbow Hatchery were also cryopreserved. The Tribe acquired 5 frozen steelhead samples from the Selway River collected in 1994 and 15 from Fish Creek sampled in 1993 from the U.S. Geological Survey, for addition into the germplasm repository. Also, 590 cryopreserved samples from the Grande Ronde chinook salmon captive broodstock program are being stored at the University of Idaho as

  8. 33 CFR 117.734 - Navesink River (Swimming River).

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Navesink River (Swimming River). 117.734 Section 117.734 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... (Swimming River). The Oceanic Bridge, mile 4.5, shall open on signal; except that, from December 1 through...

  9. Skjern River Restoration Counterfactual

    DEFF Research Database (Denmark)

    Clemmensen, Thomas Juel

    2014-01-01

    In 2003 the Skjern River Restoration Project in Denmark was awarded the prestigious Europa Nostra Prize for ‘conserving the European cultural heritage’ (Danish Nature Agency 2005). In this case, however, it seems that the conservation of one cultural heritage came at the expense of another cultural...... this massive reconstruction work, which involved moving more than 2,7 million cubic meters of earth, cause a lot of ‘dissonance’ among the local population, the resulting ‘nature’ and its dynamic processes are also constantly compromising the preferred image of the restored landscape (Clemmensen 2014......). The presentation offers insight into an on-going research and development project - Skjern River Restoration Counterfactual, which question existing trends and logics within nature restoration. The project explores how the Skjern River Delta could have been ‘restored’ with a greater sensibility for its cultural...

  10. Missouri River 1943 Compact Line

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — Flood Control, Bank Stabilization and development of a navigational channel on the Missouri River had a great impact on the river and adjacent lands. The new...

  11. Haw River PFCs Data Set

    Data.gov (United States)

    U.S. Environmental Protection Agency — PFAS concentrations in river and drinking water in and around the Haw River in North Carolina. This dataset is associated with the following publication: Sun, M., E....

  12. Baseline geochemical data for stream sediment and surface water samples from Panther Creek, the Middle Fork of the Salmon River, and the Main Salmon River from North Fork to Corn Creek, collected prior to the severe wildfires of 2000 in central Idaho

    Science.gov (United States)

    Eppinger, Robert G.; Briggs, Paul H.; Brown, Zoe Ann; Crock, James G.; Meier, Allen; Theodorakos, Peter M.; Wilson, Stephen A.

    2001-01-01

    In 1996, the U.S. Geological Survey conducted a reconnaissance baseline geochemical study in central Idaho. The purpose of the baseline study was to establish a 'geochemical snapshot' of the area, as a datum for monitoring future change in the geochemical landscape, whether natural or human-induced. This report presents the methology, analytical results, and sample descriptions for water, sediment, and heavy-mineral concentrate samples collected during this geochemical investigation. In the summer of 2000, the Clear Creek, Little Pistol, and Shellrock wildfires swept across much of the area that was sampled. Thus, these data represent a pre-fire baseline geochemical dataset. A 2001 post- fire study is planned and will involve re-sampling of the pre-fire baseline sites, to allow for pre- and post-fire comparison.

  13. Spatial scales of carbon flow in a river food web

    Science.gov (United States)

    Finlay, J.C.; Khandwala, S.; Power, M.E.

    2002-01-01

    Spatial extents of food webs that support stream and river consumers are largely unknown, but such information is essential for basic understanding and management of lotic ecosystems. We used predictable variation in algal ??13C with water velocity, and measurements of consumer ??13C and ??15N to examine carbon flow and trophic structure in food webs of the South Fork Eel River in Northern California. Analyses of ??13C showed that the most abundant macroinvertebrate groups (collector-gatherers and scrapers) relied on algae from local sources within their riffle or shallow pool habitats. In contrast, filter-feeding invertebrates in riffles relied in part on algal production derived from upstream shallow pools. Riffle invertebrate predators also relied in part on consumers of pool-derived algal carbon. One abundant taxon drifting from shallow pools and riffles (baetid mayflies) relied on algal production derived from the habitats from which they dispersed. The trophic linkage from pool algae to riffle invertebrate predators was thus mediated through either predation on pool herbivores dispersing into riffles, or on filter feeders. Algal production in shallow pool habitats dominated the resource base of vertebrate predators in all habitats at the end of the summer. We could not distinguish between the trophic roles of riffle algae and terrestrial detritus, but both carbon sources appeared to play minor roles for vertebrate consumers. In shallow pools, small vertebrates, including three-spined stickleback (Gasterosteus aculeatus), roach (Hesperoleucas symmetricus), and rough-skinned newts (Taricha granulosa), relied on invertebrate prey derived from local pool habitats. During the most productive summer period, growth of all size classes of steelhead and resident rainbow trout (Oncorhynchus mykiss) in all habitats (shallow pools, riffles, and deep unproductive pools) was largely derived from algal production in shallow pools. Preliminary data suggest that the strong

  14. Channel change and bed-material transport in the Lower Chetco River, Oregon

    Science.gov (United States)

    Wallick, J. Rose; Anderson, Scott W.; Cannon, Charles; O'Connor, Jim E.

    2010-01-01

    The lower Chetco River is a wandering gravel-bed river flanked by abundant and large gravel bars formed of coarse bed-material sediment. Since the early twentieth century, the large gravel bars have been a source of commercial aggregate for which ongoing permitting and aquatic habitat concerns have motivated this assessment of historical channel change and sediment transport rates. Analysis of historical channel change and bed-material transport rates for the lower 18 kilometers shows that the upper reaches of the study area are primarily transport zones, with bar positions fixed by valley geometry and active bars mainly providing transient storage of bed material. Downstream reaches, especially near the confluence of the North Fork Chetco River, are zones of active sedimentation and channel migration.Multiple analyses, supported by direct measurements of bedload during winter 2008–09, indicate that since 1970 the mean annual flux of bed material into the study reach has been about 40,000–100,000 cubic meters per year. Downstream tributary input of bed-material sediment, probably averaging 5–30 percent of the influx coming into the study reach from upstream, is approximately balanced by bed-material attrition by abrasion. Probably little bed material leaves the lower river under natural conditions, with most net influx historically accumulating in wider and more dynamic reaches, especially near the North Fork Chetco River confluence, 8 kilometers upstream from the Pacific Ocean.The year-to-year flux, however, varies tremendously. Some years may have less than 3,000 cubic meters of bed material entering the study area; by contrast, some high-flow years, such as 1982 and 1997, likely have more than 150,000 cubic meters entering the reach. For comparison, the estimated annual volume of gravel extracted from the lower Chetco River for commercial aggregate during 2000–2008 has ranged from 32,000 to 90,000 cubic meters and averaged about 59,000 cubic meters per

  15. Sediment transport in the lower Snake and Clearwater River Basins, Idaho and Washington, 2008–11

    Science.gov (United States)

    Clark, Gregory M.; Fosness, Ryan L.; Wood, Molly S.

    2013-01-01

    /L), and the Middle Fork Clearwater River at Kooskia, Idaho (15 mg/L). The largest measured concentrations of suspended sediment (3,300 and 1,400 mg/L) during a rain-on-snow event in January 2011 were from samples collected at the Potlatch River near Spalding, Idaho, and the Palouse River at Hooper, Washington, respectively. Generally, samples collected from agricultural watersheds had a high percentage of silt and clay-sized suspended sediment, whereas samples collected from forested watersheds had a high percentage of sand. During water years 2009–11, Lower Granite Reservoir received about 10 million tons of suspended sediment from the combined loads of the Snake and Clearwater Rivers. The Snake River accounted for about 2.97 million tons per year (about 89 percent) of the total suspended sediment, 1.48 million tons per year (about 90 percent) of the suspended sand, and about 1.52 million tons per year (87 percent) of the suspended silt and clay. Of the suspended sediment transported to Lower Granite Reservoir, the Salmon River accounted for about 51 percent of the total suspended sediment, about 56 percent of the suspended sand, and about 44 percent of the suspended silt and clay. About 6.2 million tons (62 percent) of the sediment contributed to Lower Granite Reservoir during 2009–11 entered during water year 2011, which was characterized by an above average winter snowpack and sustained spring runoff. A comparison of historical data collected from the Snake River near Anatone with data collected during this study indicates that concentrations of total suspended sediment and suspended sand in the Snake River were significantly smaller during water years 1972–79 than during 2008–11. Most of the increased sediment content in the Snake River is attributable to an increase of sand-size material. During 1972–79, sand accounted for an average of 28 percent of the suspended-sediment load; during 2008–11, sand accounted for an average of 48 percent. Historical data

  16. Stochastic Modelling of River Geometry

    DEFF Research Database (Denmark)

    Sørensen, John Dalsgaard; Schaarup-Jensen, K.

    1996-01-01

    Numerical hydrodynamic river models are used in a large number of applications to estimate critical events for rivers. These estimates are subject to a number of uncertainties. In this paper, the problem to evaluate these estimates using probabilistic methods is considered. Stochastic models for ...... for river geometries are formulated and a coupling between hydraulic computational methods and numerical reliability methods is presented....

  17. The Gediz River fluvial archive

    NARCIS (Netherlands)

    Maddy, D.; Veldkamp, A.; Demir, T.; Gorp, van W.; Wijbrans, J.R.; Hinsbergen, van D.J.J.; Dekkers, M.J.; Schreve, D.; Schoorl, J.M.; Scaife, R.

    2017-01-01

    The Gediz River, one of the principal rivers of Western Anatolia, has an extensive Pleistocene fluvial archive that potentially offers a unique window into fluvial system behaviour on the western margins of Asia during the Quaternary. In this paper we review our work on the Quaternary Gediz River

  18. Double-Forked Artifice

    DEFF Research Database (Denmark)

    Elias, Camelia

    2012-01-01

    to ignite established ideas, and blow them to pieces with his own dynamite. Quite literally, can an elastic set things on fire, survive the fire? Can a truth be final even when it is elastic? What does it mean to write, for Nietzsche, when writing presents itself as a sin against silence, as a fire...

  19. A Novel Rrm3 Function in Restricting DNA Replication via an Orc5-Binding Domain Is Genetically Separable from Rrm3 Function as an ATPase/Helicase in Facilitating Fork Progression

    DEFF Research Database (Denmark)

    Syed, Salahuddin; Madsen, Claus Desler; Rasmussen, Lene J.

    2016-01-01

    hydroxyurea. This novel Rrm3 function is independent of its established role as an ATPase/helicase in facilitating replication fork progression through polymerase blocking obstacles. Using quantitative mass spectrometry and genetic analyses, we find that the homologous recombination factor Rdh54 and Rad5...

  20. Post-closure permit application for the Upper East Fork Poplar Creek hydrogeologic regime at the Y-12 Plant: New Hope Pond and Eastern S-3 ponds plume. Revision 2

    International Nuclear Information System (INIS)

    1995-02-01

    The intent of this Post-Closure, Permit Application (PCPA) is to satisfy the post-closure permitting requirements of the Tennessee Department of Environment and Conservation (TDEC) Rule 1200-1-11. This application is for the entire Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), which is within the Bear Creek Valley (BCV). This PCPA has been prepared to include the entire East Fork Regime because, although there are numerous contaminant sources within the regime, the contaminant plumes throughout the East Fork Regime have coalesced and can no longer be distinguished as separate plumes. This PCPA focuses on two recognized Resource Conservation and Recovery Act (RCRA) interim status units: New Hope Pond (NHP) and the eastern S-3 Ponds plume. This PCPA presents data from groundwater assessment monitoring throughout the regime, performed since 1986. Using this data, this PCPA demonstrates that NHP is not a statistically discernible source of groundwater contaminants and that sites upgradient of NHP are the likely sources of groundwater contamination seen in the NHP vicinity. As such, this PCPA proposes a detection monitoring program to replace the current assessment monitoring program for NHP

  1. Geomorphic classification of rivers

    Science.gov (United States)

    J. M. Buffington; D. R. Montgomery

    2013-01-01

    Over the last several decades, environmental legislation and a growing awareness of historical human disturbance to rivers worldwide (Schumm, 1977; Collins et al., 2003; Surian and Rinaldi, 2003; Nilsson et al., 2005; Chin, 2006; Walter and Merritts, 2008) have fostered unprecedented collaboration among scientists, land managers, and stakeholders to better understand,...

  2. Savannah River Technology Center

    International Nuclear Information System (INIS)

    1993-01-01

    This is a monthly progress report from the Savannah River Laboratory for the month of January 1993. It has sections with work in the areas of reactor safety, tritium processes and absorption, separations programs and wastes, environmental concerns and responses, waste management practices, and general concerns

  3. Alligator Rivers Region

    International Nuclear Information System (INIS)

    1992-01-01

    An introduction to the Alligator Rivers Region is presented. It contains general information regarding the physiography, climate, hydrology and mining of the region. The Alligator Rivers Region is within an ancient basin, the Pine Creek Geosyncline, which has an area of approximately 66000 km 2 . The Geosyncline has a history of mineral exploitation dating back to 1865, during which time 16 metals have been extracted (silver, arsenic, gold, bismuth, cadmium, cobalt, copper, iron, manganese, molybdenum, lead, tin, tantalum, uranium, tungsten, zinc). Uranium exploration in the Pine Creek Geosyncline was stimulated by the discovery in 1949 of secondary uranium mineralisation near Rum June, 70 km south-east of Darwin. This was followed by a decade of intense exploration activity resulting in the discoveries of economic uranium ore bodies at Rum Jungle and in the upper reaches of the South Alligator River Valley. All the known major uranium deposits of the East Alligator River uranium field have been discovered since 1969. The present known resources of the Geosyncline are approximately 360 000 tonnes of contained U 3 O 8 . 2 refs., 2 figs., 1 tab

  4. Discover the Nile River

    Science.gov (United States)

    Project WET Foundation, 2009

    2009-01-01

    Bordering on the Fantastic. As the longest river on earth, the Nile passes through 10 countries. Presented through a wide range of activities and a winning array of games, it's also unsurpassed at taking young minds into exploring the world of water, as well as natural and man made wonders.

  5. Two Pontic rivers

    DEFF Research Database (Denmark)

    Bekker-Nielsen, Tønnes; Jensen, Marit

    2015-01-01

    The accounts of the landscape around the Iris (Yeşilirmak) and the Thermodon (Terme) given by ancient authors are diverse and often contradictory. The Periegesis of the World by Dionysius of Alexandria, a didactic poem written in the early IInd c. A.D., established an image of the two rivers that...

  6. River water pollution condition in upper part of Brantas River and Bengawan Solo River

    Science.gov (United States)

    Roosmini, D.; Septiono, M. A.; Putri, N. E.; Shabrina, H. M.; Salami, I. R. S.; Ariesyady, H. D.

    2018-01-01

    Wastewater and solid waste from both domestic and industry have been known to give burden on river water quality. Most of river water quality problem in Indonesia has start in the upper part of river due to anthropogenic activities, due to inappropriate land use management including the poor wastewater infrastructure. Base on Upper Citarum River Water pollution problem, it is interesting to study the other main river in Java Island. Bengawan Solo River and Brantas River were chosen as the sample in this study. Parameters assessed in this study are as follows: TSS, TDS, pH, DO, and hexavalent chromium. The status of river water quality are assess using STORET method. Based on (five) parameters, STORET value showed that in Brantas River, Pagerluyung monitoring point had the worst quality relatively compared to other monitoring point in Brantas River with exceeding copper, lead and tin compared to the stream standard in East Java Provincial Regulation No. 2 in 2008. Brantas River was categorized as lightly polluted river based on monitoring period 2011-2015 in 5 monitoring points, namely Pendem, Sengguruh, Kademangan, Meritjan and Kertosono.

  7. Ecological characterization of two species of exotic fish, pumpkinseed sunfish (Lepomis gibbosus and largemouth bass (Micropterus salmoides in the international Minho river

    Directory of Open Access Journals (Sweden)

    Ana Cristina Lages

    2015-11-01

    Full Text Available The introduction of exotic species is considered the main cause for the decline of native species. The largemouth bass (Micropterus salmoides and pumpkinseed sunfish (Lepomis gibbosus are two native species from North America, introduced in Portugal to enhance sport fishing. However, their diet and great adaptability made them considered predatory and harmful. In order to understand the ecological impact of M. salmoides and L. gibbosus in the international section of the Minho River, three sampling sites were selected: two in Vila Nova de Cerveira and one in Lapela, at distance of the mouth of the river of 17 and 45 Km, respectively. The fish were gathered using fyke nets and trammel nets, electric fishing and fishing rod, with performed samplings since July 2014 until October 2015. For all fish caught the biometric data (weight, total and fork length, gonad and liver weight, sex, stomach contents analysis were registered as well as collection of otoliths and scales for age reading. Both species feed on small macroinvertebrates specially the juveniles while adults of largemouth bass and pumpkinseed sunfish prefer eat fish and gastropods, respectively. Because L. gibbosus is a recent introduction in the Minho river estuary its abundance increased a lot in the last two years and it was possible verify the increase of the fish population average length. With this work it is intended to evaluate the impact in the Minho River estuary of both exotic species studying the population structure, trophic webs and reproduction.

  8. Water and Proppant Requirements and Water Production Associated with Undiscovered Petroleum in the Bakken and Three Forks Formations, North Dakota and Montana, USA

    Science.gov (United States)

    Haines, S. S.; Varela, B. A.; Thamke, J.; Hawkins, S. J.; Gianoutsos, N. J.; Tennyson, M. E.

    2017-12-01

    Water is used for several stages of oil and gas production, in particular for hydraulic fracturing that is typically used during production of petroleum from low-permeability shales and other rock types (referred to as "continuous" petroleum accumulations). Proppant, often sand, is also consumed during hydraulic fracturing. Water is then produced from the reservoir along with the oil and gas, representing either a disposal consideration or a possible source of water for further petroleum development or other purposes. The U.S. Geological Survey (USGS) has developed an approach for regional-scale estimation of these water and proppant quantities in order to provide an improved understanding of possible impacts and to help with planning and decision-making. Using the new methodology, the USGS has conducted a quantitative assessment of water and proppant requirements, and water production volumes, associated with associated with possible future production of undiscovered petroleum resources in the Bakken and Three Forks Formations, Williston Basin, USA. This water and proppant assessment builds directly from the 2013 USGS petroleum assessment for the Bakken and Three Forks Formations. USGS petroleum assessments incorporate all available geologic and petroleum production information, and include the definition of assessment units (AUs) that specify the geographic regions and geologic formations for the assessment. The 2013 petroleum assessment included 5 continuous AUs for the Bakken Formation and one continuous AU for the Three Forks Formation. The assessment inputs are defined probabilistically, and a Monte Carlo approach provides outputs that include uncertainty bounds. We can summarize the assessment outputs with the mean values of the associated distributions. The mean estimated total volume of water for well drilling and cement for all six continuous AUs is 5.9 billion gallons, and the mean estimated volume of water for hydraulic fracturing for all AUs is 164

  9. Verification of a ‘freshwater-type’ life history variant of juvenile American shad in the Columbia River

    Science.gov (United States)

    Wetzel, Lisa A.; Larsen, Kimberly A.; Parsley, Michael J.; Zimmerman, Christian E.

    2011-01-01

    American shad are native to the Atlantic coast of North America and were successfully introduced to the Pacific coast in the 1870s. They are now more abundant in the Columbia River than are its native salmon. As in their native range, Columbia River American shad are anadromous and have been assumed to solely exhibit an ‘ocean-type’ life history, characterized by a short period of juvenile rearing in freshwater, followed by seaward migration and saltwater entry before age-1, with sexually mature individuals returning to freshwater to spawn beginning at age-3. During October 2007, emigrating juvenile American shad were captured in the juvenile fish monitoring facility at Bonneville Dam (river kilometer 235) on the Columbia River. Their length frequencies revealed the presence of two modes; the lower mode averaged 77 mm fork length (FL) and the upper mode averaged 184 mm FL. A subsample of fish from each mode was aged using otoliths. Otoliths from the lower mode (n=10) had no annuli, indicating that they were all age-0, while otoliths from the upper mode (n=25) had one or two annuli, indicating that they were either age-1 or age-2, respectively. Spawning adults collected in June 2007 averaged 393 mm FL (range 305-460 mm; n=21) and were estimated to range in age from 3-6. Elemental analyses of juvenile and adult otoliths provide evidence for deviations from the typical migration pattern expected for this species, including extensive freshwater rearing of up to two years. This evidence shows that a ‘freshwater-type’ of juvenile American shad exists as year-round or transient residents in the Columbia River basin. The ecological role of this life history variant within the fish community is unknown.

  10. River-corridor habitat dynamics, Lower Missouri River

    Science.gov (United States)

    Jacobson, Robert B.

    2010-01-01

    Intensive management of the Missouri River for navigation, flood control, and power generation has resulted in substantial physical changes to the river corridor. Historically, the Missouri River was characterized by a shifting, multithread channel and abundant unvegetated sandbars. The shifting channel provided a wide variety of hydraulic environments and large areas of connected and unconnected off-channel water bodies.Beginning in the early 1800s and continuing to the present, the channel of the Lower Missouri River (downstream from Sioux City, Iowa) has been trained into a fast, deep, single-thread channel to stabilize banks and maintain commercial navigation. Wing dikes now concentrate the flow, and revetments and levees keep the channel in place and disconnect it from the flood plain. In addition, reservoir regulation of the Missouri River upstream of Yankton, South Dakota, has substantially changed the annual hydrograph, sediment loads, temperature regime, and nutrient budgets.While changes to the Missouri River have resulted in broad social and economic benefits, they have also been associated with loss of river-corridor habitats and diminished populations of native fish and wildlife species. Today, Missouri River stakeholders are seeking ways to restore some natural ecosystem benefits of the Lower Missouri River without compromising traditional economic uses of the river and flood plain.

  11. Streamflow monitoring and statistics for development of water rights claims for Wild and Scenic Rivers, Owyhee Canyonlands Wilderness, Idaho, 2012

    Science.gov (United States)

    Wood, Molly S.; Fosness, Ryan L.

    2013-01-01

    -record method. The synthetic and actual daily mean streamflow records were used to estimate daily mean streamflow that was exceeded 80, 50, and 20 percent of the time (80-, 50-, and 20-percent exceedances) for bimonthly and annual periods. Bankfull streamflow statistics were calculated by fitting the synthetic and actual annual peak streamflow records to a log Pearson Type III distribution using Bulletin 17B guidelines in the U.S. Geological Survey PeakFQ program. The coefficients of determination (R2) for the regressions between the monitoring and index sites ranged from 0.74 for Wickahoney Creek to 0.98 for the West Fork Bruneau River and Deep Creek. Confidence in computed streamflow statistics is highest among other sites for the East Fork Owyhee River and the West Fork Bruneau River on the basis of regression statistics, visual fit of the related data, and the range and number of streamflow measurements. Streamflow statistics for sites with the greatest uncertainty included Big Jacks, Little Jacks, Cottonwood, Wickahoney, and Sheep Creeks. The uncertainty in computed streamflow statistics was due to a number of factors which included the distance of index sites relative to monitoring sites, relatively low streamflow conditions that occurred during the study, and the limited number and range of streamflow measurements. However, the computed streamflow statistics are considered the best possible estimates given available datasets in the remote study area. Streamflow measurements over a wider range of hydrologic and climatic conditions would improve the relations between streamflow characteristics at monitoring and index sites. Additionally, field surveys are needed to verify if the streamflows selected for the water rights claims are sufficient for maintaining outstanding remarkable values in the Wild and Scenic rivers included in the study.

  12. Pesticide Occurrence and Distribution in the Lower Clackamas River Basin, Oregon, 2000-2005

    Science.gov (United States)

    Carpenter, Kurt D.; Sobieszczyk, Steven; Arnsberg, Andrew J.; Rinella, Frank A.

    2008-01-01

    Pesticide occurrence and distribution in the lower Clackamas River basin was evaluated in 2000?2005, when 119 water samples were analyzed for a suite of 86?198 dissolved pesticides. Sampling included the lower-basin tributaries and the Clackamas River mainstem, along with paired samples of pre- and post-treatment drinking water (source and finished water) from one of four drinking water-treatment plants that draw water from the lower river. Most of the sampling in the tributaries occurred during storms, whereas most of the source and finished water samples from the study drinking-water treatment plant were obtained at regular intervals, and targeted one storm event in 2005. In all, 63 pesticide compounds were detected, including 33 herbicides, 15 insecticides, 6 fungicides, and 9 pesticide degradation products. Atrazine and simazine were detected in about half of samples, and atrazine and one of its degradates (deethylatrazine) were detected together in 30 percent of samples. Other high-use herbicides such as glyphosate, triclopyr, 2,4-D, and metolachlor also were frequently detected, particularly in the lower-basin tributaries. Pesticides were detected in all eight of the lower-basin tributaries sampled, and were also frequently detected in the lower Clackamas River. Although pesticides were detected in all of the lower basin tributaries, the highest pesticide loads (amounts) were found in Deep and Rock Creeks. These medium-sized streams drain a mix of agricultural land (row crops and nurseries), pastureland, and rural residential areas. The highest pesticide loads were found in Rock Creek at 172nd Avenue and in two Deep Creek tributaries, North Fork Deep and Noyer Creeks, where 15?18 pesticides were detected. Pesticide yields (loads per unit area) were highest in Cow and Carli Creeks, two small streams that drain the highly urban and industrial northwestern part of the lower basin. Other sites having relatively high pesticide yields included middle Rock Creek and

  13. An archaeological reconnaissance of a 14 mile section of the East Fork Poplar Creek for the Environmental Restoration Project, Anderson and Roane Counties, Tennessee

    International Nuclear Information System (INIS)

    DuVall, G.D.

    1993-01-01

    At the request of the US Army Corps of Engineers, Nashville District, Nashville, Tennessee, an archaeological reconnaissance of the potential impact areas of the Environmental Restoration Project (ERP) along the East Fork Poplar Creek was conducted during the period December 16, 1991, and March 3, 1992. The reconnaissance was conducted in response to environmental evaluations as a result of the accidental spillage of approximately 293,000 pounds of mercury, radionuclides, heavy metals and other compounds. The reconnaissance to assess adverse impacts to cultural resources located within the boundaries of Federally-licensed, permitted, funded or assisted projects was conducted in compliance with the National Historic Preservation Act of 1966, National Environmental Policy Act of 1969 and Executive Order 11593

  14. Remedial investigation work plan for the Upper East Fork Poplar Creek characterization area, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-09-01

    The Oak Ridge Y-12 Plant, located within the Oak Ridge Reservation (ORR), is owned by the US Department of Energy (DOE) and managed by Lockheed Martin Energy Systems, Inc. The entire ORR was placed on the National Priorities List (NPL) of CERCLA sites in November 1989. Following CERCLA guidelines, sites under investigation require a remedial investigation (RI) to define the nature and extent of contamination, evaluate the risks to public health and the environment, and determine the goals for a feasibility study (FS) of potential remedial actions. The need to complete RIs in a timely manner resulted in the establishment of the Upper East Fork Poplar Creek (UEFPC) Characterization Area (CA) and the Bear Creek CA. The CA approach considers the entire watershed and examines all appropriate media within it. The UEFPC CA, which includes the main Y-12 Plant area, is an operationally and hydrogeologically complex area that contains numerous contaminants and containment sources, as well as ongoing industrial and defense-related activities. The UEFPC CA also is the suspected point of origin for off-site groundwater and surface-water contamination. The UEFPC CA RI also will address a carbon-tetrachloride/chloroform-dominated groundwater plume that extends east of the DOE property line into Union Valley, which appears to be connected with springs in the valley. In addition, surface water in UEFPC to the Lower East Fork Poplar Creek CA boundary will be addressed. Through investigation of the entire watershed as one ''site,'' data gaps and contaminated areas will be identified and prioritized more efficiently than through separate investigations of many discrete units.

  15. Spatial and temporal distribution of bull trout (Salvelinus confluentus)-size fish near the floating surface collector in the North Fork Reservoir, Oregon, 2016

    Science.gov (United States)

    Adams, Noah S.; Smith, Collin D.

    2017-06-26

    Acoustic cameras were used to assess the behavior and abundance of bull trout (Salvelinus confluentus)-size fish at the entrance to the North Fork Reservoir juvenile fish floating surface collector (FSC). The purpose of the FSC is to collect downriver migrating juvenile salmonids at the North Fork Dam, and safely route them around the hydroelectric projects. The objective of the acoustic camera component of this study was to assess the behaviors of bull trout-size fish observed near the FSC, and to determine if the presence of bull trout-size fish influenced the collection or abundance of juvenile salmonids. Acoustic cameras were deployed near the surface and floor of the entrance to the FSC. The acoustic camera technology was an informative tool for assessing abundance and spatial and temporal behaviors of bull trout-size fish near the entrance of the FSC. Bull trout-size fish were regularly observed near the entrance, with greater abundances on the deep camera than on the shallow camera. Additionally, greater abundances were observed during the hours of sunlight than were observed during the night. Behavioral differences also were observed at the two depths, with surface fish traveling faster and straighter with more directed movement, and fish observed on the deep camera generally showing more milling behavior. Modeling potential predator-prey interactions and influences using collected passive integrated transponder (PIT) -tagged juvenile salmonids proved largely unpredictable, although these fish provided relevant timing and collection information. Overall, the results indicate that bull trout-size fish are present near the entrance of the FSC, concomitant with juvenile salmonids, and their abundances and behaviors indicate that they may be drawn to the entrance of the FSC because of the abundance of prey-sized fish.

  16. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage.

    Science.gov (United States)

    Waraky, Ahmed; Lin, Yingbo; Warsito, Dudi; Haglund, Felix; Aleem, Eiman; Larsson, Olle

    2017-11-03

    We have previously shown that the insulin-like growth factor 1 receptor (IGF-1R) translocates to the cell nucleus, where it binds to enhancer-like regions and increases gene transcription. Further studies have demonstrated that nuclear IGF-1R (nIGF-1R) physically and functionally interacts with some nuclear proteins, i.e. the lymphoid enhancer-binding factor 1 (Lef1), histone H3, and Brahma-related gene-1 proteins. In this study, we identified the proliferating cell nuclear antigen (PCNA) as a nIGF-1R-binding partner. PCNA is a pivotal component of the replication fork machinery and a main regulator of the DNA damage tolerance (DDT) pathway. We found that IGF-1R interacts with and phosphorylates PCNA in human embryonic stem cells and other cell lines. In vitro MS analysis of PCNA co-incubated with the IGF-1R kinase indicated tyrosine residues 60, 133, and 250 in PCNA as IGF-1R targets, and PCNA phosphorylation was followed by mono- and polyubiquitination. Co-immunoprecipitation experiments suggested that these ubiquitination events may be mediated by DDT-dependent E2/E3 ligases ( e.g. RAD18 and SHPRH/HLTF). Absence of IGF-1R or mutation of Tyr-60, Tyr-133, or Tyr-250 in PCNA abrogated its ubiquitination. Unlike in cells expressing IGF-1R, externally induced DNA damage in IGF-1R-negative cells caused G 1 cell cycle arrest and S phase fork stalling. Taken together, our results suggest a role of IGF-1R in DDT. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Umatilla River Fish Passage Operations Project : Annual Progress Report October 2007 - September 2008.

    Energy Technology Data Exchange (ETDEWEB)

    Bronson, James P.; Loffink, Ken; Duke, Bill

    2008-12-31

    Threemile Falls Dam (Threemile Dam), located near the town of Umatilla, is the major collection and counting point for adult salmonids returning to the Umatilla River. Returning salmon and steelhead were enumerated at Threemile Dam from June 7, 2007 to August 11, 2008. A total of 3,133 summer steelhead (Oncorhynchus mykiss); 1,487 adult, 1,067 jack, and 999 subjack fall Chinook (O. tshawytscha); 5,140 adult and 150 jack coho (O. kisutch); and 2,009 adult, 517 jack, and 128 subjack spring Chinook (O. tshawytscha) were counted. All fish were enumerated at the east bank facility. Of the fish counted, 1,442 summer steelhead and 88 adult and 84 jack spring Chinook were hauled upstream from Threemile Dam. There were 1,497 summer steelhead; 609 adult, 1,018 jack and 979 subjack fall Chinook; 5,036 adult and 144 jack coho; and 1,117 adult, 386 jack and 125 subjack spring Chinook either released at, or allowed to volitionally migrate past, Threemile Dam. Also, 110 summer steelhead; 878 adult and 43 jack fall Chinook; and 560 adult and 28 jack spring Chinook were collected as broodstock for the Umatilla River hatchery program. In addition, there were 241 adult and 15 jack spring Chinook collected at Threemile Dam for outplanting in the South Fork Walla Walla River and Mill Cr, a tributary of the mainstem Walla Walla River. The Westland Canal juvenile facility (Westland), located near the town of Echo at river mile (RM) 27, is the major collection point for out-migrating juvenile salmonids and steelhead kelts. The canal was open for 158 days between February 11, 2008 and July 18, 2008. During that period, fish were bypassed back to the river 150 days and were trapped 6 days. There were also 2 days when fish were directed into and held in the canal forebay between the time the bypass was closed and the trap opened. An estimated 64 pounds of fish were transported from the Westland trapping facility. Approximately 25.8% of the fish transported were salmonids. In addition, one

  18. Identifying Groundwater Discharge in the Merced River Basin, California Using Radon-222

    Science.gov (United States)

    Shaw, G. D.; Hudson, G. B.; Moran, J.; Conklin, M.

    2004-12-01

    Groundwater flow in fractured granite of the Sierra Nevada is poorly characterized, in particular, contributions of mountain block recharge are not known. Using a combination of water quality and isotopic analyses, groundwater inputs to the Upper Merced River were characterized. Between November 2003 and July 2004, monthly water quality samples were taken from Happy Isles to the inlet of Lake McClure, a 75 km reach. These samples demonstrated the expected dilution due to snowmelt in the spring. In the fall, the spatial profile matched the geology with anion concentrations increasing downstream of the transition from the Sierra Nevada batholith to the country rock, suggesting significant groundwater inputs. From July 19 to 21, 2004, radon-222 and other noble gases (He, Ne, Ar, Kr and Xe abundances and 3He/4He ratio) were measured along a 37 km reach of the Merced River, extending from the top of Yosemite Valley to the confluence of the South Fork of the Merced River. All radon samples were extracted into mineral oil immediately in the field and counted using liquid scintillation; noble gas samples were collected in copper tubes. Radon-222 activity varied from about 1 to 100 pCi/L (at collection time) indicating significant, spatially variable groundwater discharge into the Merced River. Two one-mile reaches of the Merced River were sampled for 222Rn on a fine scale. Large fracture sets in these two locations and previous temperature measurements suggested that groundwater discharge was higher relative to other locations along the river. Radon-222 activity was low upstream and downstream of large fractures observed in the bedrock; whereas, 222Rn activity was high at large fracture zones. Degassing is rapid downstream of fractures where no groundwater discharge is observed. For a representative groundwater end-member, radon-222 activity measured in Fern Spring, Yosemite Valley was about 1200 pCi/L. Excess 4He from U and Th decay is observed in samples with elevated

  19. Sapucai River Project

    International Nuclear Information System (INIS)

    Duarte, A.L.; Rosa, M.J.

    1988-01-01

    The Sapucai River Project is a gold, ilmenite, monazite and zircon alluvial deposit. It is located on Sapucai River valley in the south of Minas Gerais State. The reserves are 28.000.000 m 3 of pay bed. The production will be 1.400.000 m 3 /year and the mine's life 20 years. A cutterhead suction dredge will do the overburden removal. The pay bed will be mined with an underwater bucket-wheel dredge. The ROM will be concentrated in a washing plant. The gold will be recovered by leaching method. The other heavy minerals will be recovered by electrostatic, magnetic and gravitic methods. SAMITRI believes that it's possible to implant and operate the Project without ecological damage. (author) [pt

  20. Geomorphology and River Management

    Directory of Open Access Journals (Sweden)

    GARY BRIERLEY

    2008-01-01

    Full Text Available Engineering-dominated practices, visible in a "command and control" outlook on natural systems, have induced enormous damage to the environment. Biodiversity losses and declining provision of ecosystem services are testimony to the non-sustainable outcomes brought about by such practices. More environmentally friendly approaches that promote a harmonious relationship between human activities and nature are required. Moves towards an "ecosystem approach" to environmental management require coherent (integrative scientific guidance. Geomorphology, the study of the form of the earth, provides a landscape template with which to ground this process. This way of thinking respects the inherent diversity and complexity of natural systems. Examples of the transition toward such views in environmental practice are demonstrated by the use of science to guide river management, emphasising applications of the River Styles framework.