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Sample records for reseau htr froid

  1. L’émoi envahissant et le sang-froid

    OpenAIRE

    2008-01-01

    « L’émoi envahissant et le sang-froid ». Le propos de cette étude est de comparer dans une perspective sémiotique les considérations générales qui clôturent l’article de Mauss sur « Les tech­niques du corps » dans lesquelles l’auteur insiste sur le sang froid comme ré­sultat-modèle d’une « technique d’adaptation du corps à son usage », aboutissement d’une « résistance à l’émoi envahissant », avec les propos te­nus sur le même sujet par A. Damasio dans L’Erreur de Descartes. L’objectif est d’a...

  2. Present status of Indonesia HTR team activities

    Energy Technology Data Exchange (ETDEWEB)

    Lasman, As Natio; Arbie, Bakri [National Atomic Energy Agency BATAN, Jakarta (Indonesia)

    1998-09-01

    The Indonesian HTR Team was established in August 1993 to conduct studies on HTR technology and its application. The team is divided into 2 groups, i.e. reactor technology and safety group, which includes activities in the field of neutronics, thermohydraulics, fuel elements, information and communication, environmental, HTR material, techno-economical aspect, and application group. Especially in the field of application, the study is connected with CO2 conversion from the enriched CO2 of the Natuna gas field by using HTR. This activity is now influenced by another program for the Natuna gas field and the Memberamo river in Irian Jaya. Another activity concerns coal liquefaction because of the relative big abundance of coal resources in Indonesia. Coal are mostly utilized for electric power generation and for cement industries. Regarding the prediction that Indonesia is becoming one of the nett oil importing countries, the coal liquefaction is needed and will be realized if and only if the fluid fuel from coal is competitive. The study activity in desalination is still done by using an HTR or floating nuclear power plant, it is especially addressed for the eastern part of Indonesia. Particle coatings activity is still done in Yogyakarta Nuclear Research Center since 3 years ago, and it will be supported in the near future in the Center for Nuclear Fuel Element at Serpong. 3 refs.

  3. Reconnaissance Invariante des Formes avec le Filtre de Fourier-Mellin et un Reseau Neuronique

    Science.gov (United States)

    Lejeune, Claude

    Le filtre de Fourier-Mellin est applique a un ensemble d'objets pour generer des vecteurs invariants sous translation, rotation et changement d'echelle. C'est la premiere methode permettant d'obtenir ces trois invariants simultanement. Le calcul des vecteurs invariants est fait numeriquement et optiquement. Les vecteurs ainsi obtenus sont utilises comme entrees dans un reseau neuronique backpropagation pour faire la classification des prototypes qui lui sont presentes. Les dimensions des vecteurs invariants sont tres petites par rapport aux objets d'entree et permettent d'utiliser un reseau possedant un nombre restreint de connexions. Il devient possible d'entrai ner le reseau dans des temps relativement courts sur un ordinateur du type PC. Une fois le reseau entrai ne, nous lui presentons des vecteurs invariants provenant d'objets se retrouvant dans l'ensemble d'entrai nement mais ayant subi des rotations et des changements d'echelle. Ce nouveau groupe represente l'ensemble de rappel. La performance de la methode est tres bonne avec des taux de succes superieurs a 85%.

  4. HTR-TN a European network for the development of HTR technology

    Energy Technology Data Exchange (ETDEWEB)

    Von Lensa, W. [Forschungszentrum Juelich GmbH (Germany). Inst. fuer Sicherheitsforschung und Reaktortechnik; Guidez, J. [Joint Research Centre, JRC-IAM, HFR unit, Petten (Netherlands)

    2001-07-01

    A network called High-temperature reactor technology network (HTR-TN) has been created at a European level to coordinate works and knowledge on the subject with a long-term perspective and to serve as a channel for international collaboration. An analysis confirmed that the obvious economic penalty of HTR due to its low density power could be compensated by the combination of recent advances that may completely change the positioning of HTR on the energy market: -) the modular concept allowed to get a reactor free from core melt risk without intervention of any active safety system, implying a drastic simplification of the design of the reactor and the safety systems as well as a standardisation and potential for shop fabrication in series; -) the development of gas turbines, the efficiency of which increased, in 10 years, from 35% till 50% and more, enabling to consider suppression of the secondary system; -) the ultra high burn-up potential of HTR fuel and the possibility for direct disposal of spent HTR fuel elements that may reduce cost of the fuel cycle and contribute to the reduction of civil and military plutonium stockpiles. (A.C.)

  5. Régulation en génie climatique froid, climatisation, chauffage

    CERN Document Server

    Desmons, Jean

    2010-01-01

    Après un rappel des bases de l'électronique et de l'hydraulique nécessaires en régulation climatique, cet ouvrage donne tous les éléments méthodologiques et technologiques pour : concevoir les systèmes de régulation adaptés aux installations de froid, de climatisation et de chauffage ; mettre en oeuvre les régulateurs (entrées / sorties, réglage, paramétrage, dépannage, repérage et contrôle) ; pratiquer la maintenance des installations. Il regroupe notamment plusieurs cas pratiques de régulation d'installations réelles : groupe à eau glacée, bloc opératoire, pompes à chaleur, régulation numérique, détente directe, alimentation en fluide frigoporteur. Cette seconde édition révisée et mise à jour s'enrichit également d'une section sur les installations photovoltaïques et d'un nouveau chapitre sur la régulation numérique et les systèmes informatiques. Cet ouvrage constitue un outil de travail indispensable aux ingénieurs et techniciens en froid et génie climatique et en régu...

  6. Associations of serotonin receptor gene HTR3A, HTR3B, and HTR3A haplotypes with bipolar disorder in Chinese patients.

    Science.gov (United States)

    Jian, J; Li, C; Xu, J; Qiao, D; Mi, G; Chen, X; Tang, M

    2016-09-16

    Single nucleotide polymorphisms (SNPs) in HTR3A and HTR3B have been reported to be associated with bipolar disorder in European and Japanese populations. We explored the roles of 21 tag SNPs in HTR3A and HTR3B in susceptibility to bipolar disorder in a Chinese cohort. Twenty-one Tag SNPs were genotyped in a study consisting of 130 patients with bipolar disorder, who visited Shandong Mental Health Center between June 2013 and May 2014, and 109 healthy individuals as controls. All of the tag SNPs were genotyped using Sequenom MassArray matrix-assisted laser desorption/ionization time of flight spectrometry. Plink 1.07, Haploview 4.2, and SPSS 20.0 were used for the analysis of the genotypes and the associations of the haplotypes with bipolar disorder. Association analyses of tag SNPs detected significant associations with the A allele in HTR3A rs1176719 (P = 0.030) and the C allele in HTR3A rs1176713 (P = 0.048). Haplotype-based association analyses indicated a statistically significant (P = 0.035) five-SNP haplotype (rs1062613:C, rs11604247:C, rs1176722:G, rs2276302:A, rs1176719:G) of linkage disequilibrium in block 3. Analysis of our small Chinese sample revealed a significant association of HTR3A with bipolar disorder, but yielded no evidence of an association between HTR3B and bipolar disorder. Furthermore, evidence for an association was found for a haplotype of HTR3A. Studies with larger Chinese samples are needed to verify our findings.

  7. Graphite Oxidation Simulation in HTR Accident Conditions

    Energy Technology Data Exchange (ETDEWEB)

    El-Genk, Mohamed

    2012-10-19

    Massive air and water ingress, following a pipe break or leak in steam-generator tubes, is a design-basis accident for high-temperature reactors (HTRs). Analysis of these accidents in both prismatic and pebble bed HTRs requires state-of-the-art capability for predictions of: 1) oxidation kinetics, 2) air helium gas mixture stratification and diffusion into the core following the depressurization, 3) transport of multi-species gas mixture, and 4) graphite corrosion. This project will develop a multi-dimensional, comprehensive oxidation kinetics model of graphite in HTRs, with diverse capabilities for handling different flow regimes. The chemical kinetics/multi-species transport model for graphite burning and oxidation will account for temperature-related changes in the properties of graphite, oxidants (O2, H2O, CO), reaction products (CO, CO2, H2, CH4) and other gases in the mixture (He and N2). The model will treat the oxidation and corrosion of graphite in geometries representative of HTR core component at temperatures of 900°C or higher. The developed chemical reaction kinetics model will be user-friendly for coupling to full core analysis codes such as MELCOR and RELAP, as well as computational fluid dynamics (CFD) codes such as CD-adapco. The research team will solve governing equations for the multi-dimensional flow and the chemical reactions and kinetics using Simulink, an extension of the MATLAB solver, and will validate and benchmark the model's predictions using reported experimental data. Researchers will develop an interface to couple the validated model to a commercially available CFD fluid flow and thermal-hydraulic model of the reactor , and will perform a simulation of a pipe break in a prismatic core HTR, with the potential for future application to a pebble-bed type HTR.

  8. Two Phase Flow Stability in the HTR-10 Steam Generator

    Institute of Scientific and Technical Information of China (English)

    居怀明; 左开芬; 刘志勇; 徐元辉

    2001-01-01

    A 10 MW High Temperature Gas Cooled Reactor (HTR-10) designed bythe Institute of Nuclear Energy Technology (INET) is now being constructed. The steam generator (SG) in the HTR-10 is one of the most important components for reactor safety. The thermal-hydraulic performance of the SG was investigated. A full scale HTR-10 Steam Generator Two Tube Engineering Model Test Facility (SGTM-10) was installed and tested at INET. This paper describes the SGTM-10 thermal hydraulic experimental system in detail. The SGTM-10 simulates the actual thermal and structural parameters of the HTR-10. The SGTM-10 includes three separated loops: the primary helium loop, the secondary water loop, and the tertiary cooling water loop. Two parallel tubes are arranged in the test assembly. The main experimental equipment is shown in the paper. Expermental results are given illustrating the effects of the outlet pressures, the heating power, and the inlet subcooling.

  9. Nuclear Safeguards Approach Consideration for HTR With Pebble Fuels

    Institute of Scientific and Technical Information of China (English)

    LIU; Hong-bin; MIAO; Qiang

    2013-01-01

    High temperature reactor(HTR)is considered to be a kind of fourth generation nuclear energy with advanced technology.It is typically safety,high efficiency and applicable in many areas.HTR has beenlisted as one of 16 significant development projects in the Middle and Long Term National Science and Technology Development Projects(2006—2020).On the basis of the research and development of

  10. Sang Froid in a time of trouble: is a vaccine against HIV possible?

    Directory of Open Access Journals (Sweden)

    Plotkin Stanley A

    2009-02-01

    Full Text Available Abstract Since the announcement of the STEP trial results in the past months, we have heard many sober pronouncements on the possibility of an HIV vaccine. On the other hand, optimistic quotations have been liberally used, from Shakespeare's Henry V's "Once more unto the breach, dear friends" to Winston Churchill's definition of success as "going from one failure to another with no loss of enthusiasm". I will forgo optimistic quotations for the phrase "Sang Froid", which translates literally from the French as "cold blood"; what it really means is to avoid panic when things look bad, to step back and coolly evaluate the situation. This is not to counsel easy optimism or to fly in face of the facts, but I believe that while the situation is serious, it is not desperate. I should stipulate at the outset that I am neither an immunologist nor an expert in HIV, but someone who has spent his life in vaccine development. What I will try to do is to provide a point of view from that experience. There is no doubt that the results of STEP were disappointing: not only did the vaccine fail to control viral load, but may have adversely affected susceptibility to infection. But HIV is not the only vaccine to experience difficulties; what lessons can we glean from prior vaccine development?

  11. 5-HTR1A and 5-HTR2A genetic polymorphisms and SSRI antidepressant response in depressive Chinese patients

    Directory of Open Access Journals (Sweden)

    Dong ZQ

    2016-07-01

    Full Text Available Zai-Quan Dong,1,* Xi-Rong Li,2,* Lin He,3,4 Guang He,3,4 Tao Yu,3,4 Xue-Li Sun1 1Psychological Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 2Mental Health Center, First Affiliated Hospital, Chongqing Medical University, Chongqing, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education, Shanghai Jiao Tong University, 4Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Objective: Genetic variabilities within the serotoninergic system may predict response or remission to antidepressant drugs. Several serotonin receptor (5-HTR gene polymorphisms have been associated with susceptibility to psychiatric diseases. In this study, we analyzed the correlation between 5-HTR1A and 5-HTR2A polymorphisms and response or remission to selective serotonin reuptake inhibitors (SSRIs drugs. Methods: Two hundred and ninety patients who met the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition criteria for major depressive disorder were involved in this study. SSRIs (fluoxetine, paroxetine, citalopram, or sertraline were selected randomly for treatment. The Hamilton Rating Scale for Depression was used to evaluate the antidepressant effect. To assess 5-HTR gene variabilities, two single-nucleotide polymorphisms in 5-HTR1A (rs1364043 and rs10042486 and three in 5-HTR2A (rs6311, rs6313, and rs17289304 were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the Sequenom MassARRAY Analyzer 4 system. Results: There were 220 responders and 70 nonresponders (120 remissioners and 170 nonremissioners after 6 weeks of treatment. We found no association between any of the five 5-HTR1A and 5-HTR2A gene polymorphisms and antidepressant drug response or remission (P>0

  12. Quantitative analysis of uncertainty from pebble flow in HTR

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Hao, E-mail: haochen.heu@163.com [Fundamental Science on Nuclear Safety and Simulation Technology Laboratory, College of Nuclear Science and Technology, Harbin Engineering University, Harbin (China); Institute of Nuclear and New Energy Technology (INET), Collaborative Innovation Center of Advanced Nuclear Energy Technology, Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education, Tsinghua University, Beijing (China); Fu, Li; Jiong, Guo; Ximing, Sun; Lidong, Wang [Institute of Nuclear and New Energy Technology (INET), Collaborative Innovation Center of Advanced Nuclear Energy Technology, Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education, Tsinghua University, Beijing (China)

    2015-12-15

    Highlights: • An uncertainty and sensitivity analysis model for pebble flow has been built. • Experiment and random walk theory are used to identify uncertainty of pebble flow. • Effects of pebble flow to the core parameters are identified by sensitivity analysis. • Uncertainty of core parameters due to pebble flow is quantified for the first time. - Abstract: In pebble bed HTR, along the deterministic average flow lines, randomness exists in the flow of pebbles, which is not possible to simulate with the current reactor design codes for HTR, such as VSOP, due to the limitation of current computer capability. In order to study how the randomness of pebble flow will affect the key parameters in HTR, a new pebble flow model was set up, which has been successfully transplanted into the VSOP code. In the new pebble flow model, mixing coefficients were introduced into the fixed flow line to simulate the randomness of pebble flow. Numerical simulation and pebble flow experiments were facilitated to determine the mixing coefficients. Sensitivity analysis was conducted to achieve the conclusion that the key parameters of pebble bed HTR are not sensitive to the randomness in pebble flow. The uncertainty of maximum power density and power distribution caused by the randomness in pebble flow is very small, especially for the “multi-pass” scheme of fuel circulation adopted in the pebble bed HTR.

  13. Gas reactor international cooperative program. HTR-synfuel application assessment

    Energy Technology Data Exchange (ETDEWEB)

    1979-09-01

    This study assesses the technical, environmental and economic factors affecting the application of the High Temperature Gas-Cooled Thermal Reactor (HTR) to: synthetic fuel production; and displacement of fossil fuels in other industrial and chemical processes. Synthetic fuel application considered include coal gasification, direct coal liquefaction, oil shale processing, and the upgrading of syncrude to motor fuel. A wide range of other industrial heat applications was also considered, with emphasis on the use of the closed-loop thermochemical energy pipeline to supply heat to dispersed industrial users. In this application syngas (H/sub 2/ +CO/sub 2/) is produced at the central station HTR by steam reforming and the gas is piped to individual methanators where typically 1000/sup 0/F steam is generated at the industrial user sites. The products of methanation (CH/sub 4/ + H/sub 2/O) are piped back to the reformer at the central station HTR.

  14. Optical lattice clock with strontium atoms: a second generation of cold atom clocks; Horloge a reseau optique au strontium: une 2. generation d'horloges a atomes froids

    Energy Technology Data Exchange (ETDEWEB)

    Le Targat, R

    2007-07-15

    Atomic fountains, based on a microwave transition of Cesium or Rubidium, constitute the state of the art atomic clocks, with a relative accuracy close to 10{sup -16}. It nevertheless appears today that it will be difficult to go significantly beyond this level with this kind of device. The use of an optical transition, the other parameters being unchanged, gives hope for a 4 or 5 orders of magnitude improvement of the stability and of the relative uncertainty on most systematic effects. As for motional effects on the atoms, they can be controlled on a very different manner if they are trapped in an optical lattice instead of experiencing a free ballistic flight stage, characteristic of fountains. The key point of this approach lies in the fact that the trap can be operated in such a way that a well chosen, weakly allowed, J=0 {yields} J=0 clock transition can be free from light shift effects. In this respect, the strontium atom is one of the most promising candidate, the 1S{sub 0} {yields} 3P{sub 0} transition has a natural width of 1 mHz, and several other easily accessible transitions can be used to efficiently laser cool atoms down to 10 {mu}K. This thesis demonstrates the experimental feasibility of an optical lattice clock based on the strontium atom, and reports on a preliminary evaluation of the relative accuracy with the fermionic isotope {sup 87}Sr, at a level of a few 10{sup -15}. (author)

  15. The family of Deg/HtrA proteases in plants

    Directory of Open Access Journals (Sweden)

    Schuhmann Holger

    2012-04-01

    Full Text Available Abstract Background The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms from bacteria to human and vascular plants. In recent years, multiple deg/htrA protease genes were identified in various plant genomes. During genome annotations most proteases were named according to the order of discovery, hence the same names were sometimes given to different types of Deg/HtrA enzymes in different plant species. This can easily lead to false inference of individual protease functions based solely on a shared name. Therefore, the existing names and classification of these proteolytic enzymes does not meet our current needs and a phylogeny-based standardized nomenclature is required. Results Using phylogenetic and domain arrangement analysis, we improved the nomenclature of the Deg/HtrA protease family, standardized protease names based on their well-established nomenclature in Arabidopsis thaliana, and clarified the evolutionary relationship between orthologous enzymes from various photosynthetic organisms across several divergent systematic groups, including dicots, a monocot, a moss and a green alga. Furthermore, we identified a “core set” of eight proteases shared by all organisms examined here that might provide all the proteolytic potential of Deg/HtrA proteases necessary for a hypothetical plant cell. Conclusions In our proposed nomenclature, the evolutionarily closest orthologs have the same protease name, simplifying scientific communication when comparing different plant species and allowing for more reliable inference of protease functions. Further, we proposed that the high number of Deg/HtrA proteases in plants is mainly due to gene duplications unique to the respective organism.

  16. Immunohistochemical Analysis of Omi/HtrA2 Expression in Prostate Cancer and Benign Prostatic Hyperplasia

    Institute of Scientific and Technical Information of China (English)

    HU Xiaoyong; CHEN Xiaochun; PING Hao; CHEN Zhaohui; ZENG Fuqing; LU Gongcheng

    2005-01-01

    To study the expression and significance of the serine protease Omi/HtrA2 in prostate cancer and benign prostatic hyperplasia. The expression of Omi/HtrA2 was assayed by means of immunohistochemical technique in 41 prostate cancer (Cap), 20 benign prostatic hyperplasia (BPH) and 10 normal prostate (NP) specimens. Omi/HtrA2 expression was positive in 30 (73.17%) prostate cancer specimens, and the positive rate of Omi/HtrA2 was lower in well differentiated than in poorly and moderately differentiated groups (P<0.05). By contrast, the cells in normal prostate and benign prostatic hyperplasia groups showed no or weak expression of Omi/HtrA2.Prostate cancer cells in vivo may need Omi/HtrA2 expression for apoptosis, and that Omi/HtrA2expression might be involved in prostate cancer development.

  17. Assessment of LWR-HTR-GCFR Integrated Cycle

    Directory of Open Access Journals (Sweden)

    Eleonora Bomboni

    2009-01-01

    Full Text Available Preliminary analyses already performed showed that innovative GCRs, both thermal and fast, are very promising candidate to reach the Gen-IV sustainability goal. The integrated LWR-HTR-GCFR basically aims at closing the current nuclear fuel cycle: in principle, thanks to the unique characteristics of Helium coolant reactors, LWR SNF along with DU become valuable material to produce energy. Additionally, burning HMs of LWR SNF means not only a drastic reduction in the Unat demand but also a remarkable decrease in the long-term radiotoxic component of nuclear waste to be geologically stored. This paper focuses on the analyses of the LWR-HTR-GCFR cycle performed by the University of Pisa in the frame of the EU PUMA project (6th FP. Starting from a brief outline of the main characteristics of HTR and GCFR concepts and of the advantages of linking LWR, HTR and GCFR in a symbiotic way, this paper shows the integrated cycle involving a typical LWR (1000 MWe, a PBMR (400 MWth and a GCFR-“E” (2400 MWth. Additionally, a brief overview of the main technological constraints concerning (Pu+MA-based advanced fuels is given, in order to explain and justify the choices made in the framework of the considered cycle. Thereafter, calculations performed and results obtained are described.

  18. Genetic and bibliographic information: HTR2B [GenLibi

    Lifescience Database Archive (English)

    Full Text Available HTR2B 5-hydroxytryptamine (serotonin) receptor 2B human alcoholism (MeSH) Disorders... of Environmental Origin (C21) > Substance-Related Disorders (C21.739) > Alcohol-Related Disorders (C21.739.100) > Alcoholism (C21.739.100.250) 01A0634580 ...

  19. Study on HTR-PM Radioactive Waste Minimization Strategy

    Institute of Scientific and Technical Information of China (English)

    JIANG; Zi-ying; ZHANG; Yan-qi; WEN; Bao-yin; LI; Hong; LIAO; Hai-tao

    2015-01-01

    The high temperature gas-cooled reactor pebble bed module(HTR-PM)in constructionhas the characteristics of the fourth generation reactors and it is different both the reactor structure and the radioactive wastes generation from the PWR NPPs in operation at present in China.

  20. Reactor Physics Characterization of the HTR Module with UCO Fuel

    Energy Technology Data Exchange (ETDEWEB)

    Gerhard Strydom

    2011-01-01

    ABSTRACT The HTR Module [1] is a graphite-moderated, helium cooled pebble bed High Temperature Reactor (HTR) design that has been extensively used as a reference template for the former South African and current Chinese HTR [2] programs. This design utilized spherical fuel elements packed into a dynamic pebble bed, consisting of TRISO coated uranium oxide (UO2) fuel kernels with a U-235 enrichment of 7.8% and a Heavy Metal loading of 7 grams per pebble. The main objective of this study is to compare several important reactor physics and core design parameters for the HTR Module and an identical design utilizing UCO fuel kernels. Fuel kernels of this type are currently being tested in the Idaho National Laboratory’s (INL) Advanced Test Reactor (ATR) as part of the larger Next Generation Nuclear Plant (NGNP) project. The PEBBED-THERMIX [3] code, which was developed specifically for the analysis of pebble bed HTRs, was used to compare the coupled neutronic and thermal fluid performance of the two designs.

  1. Improvements in the fabrication of HTR fuel elements

    Energy Technology Data Exchange (ETDEWEB)

    Braehler, Georg, E-mail: georg.braehler@nukemtechnologies.de [NUKEM Technologies GmbH, Industriestrasse 13, 63755 Alzenau (Germany); Hartung, Markus [NUKEM Technologies GmbH, Industriestrasse 13, 63755 Alzenau (Germany); Fachinger, Johannes; Grosse, Karl-Heinz [FNAG Furnaces Nuclear Applications Grenoble S.A.S., Wilhelm-Rohn Strasse 35, 63450 Hanau (Germany); Seemann, Richard [ALD Vacuum Technologies GmbH, Wilhelm-Rohn Strasse 35, 63450 Hanau (Germany)

    2012-10-15

    The application of High Temperature Reactor (HTR) Technology in the course of the continuously increasing world wide demand on energy is taken more and more under serious consideration in the power supply strategy of various countries. Especially for the emerging nations the HTR Technology has become of special interest because of its inherent safety feature and due to the alternative possibilities of applications, e.g. in the production of liquid hydrocarbons or the alternative application in H{sub 2} generation. The HTR fuel in its various forms (spheres or prismatic fuel blocks) is based on small fuel kernels of about 500 {mu}m in diameter. Each of these uranium oxide or carbide kernels are coated with several layers of pyrocarbon (PyC) as well as an additional silicon carbide (SiC) layer. While the inner pyrocarbon layer is porous and capable to absorb gaseous fission products, the dense outer PyC layer forms the barrier against fission product release. The SiC layer improves the mechanical strengths of this barrier and considerably increases the retention capacity for solid fission products that tent to diffuse at these temperatures. Especially the high quality German LEU TRISO spherical fuel based on the NUKEM design, has demonstrated the best fission product release rate, particular at high temperatures. The {approx}10% enriched uranium triple-coated particles are embedded in a moulded graphite sphere. A fuel sphere consists of approximately 9 g of uranium (some 15,000 particles) and has a diameter of 60 mm. As the unique safety features, especially the inherent safety of the HTR is based on the fuel design, this paper shall reflect the complexity but also developments and economical aspects of the fabrication processes for HTR fuel elements.

  2. Serine protease HtrA1 expression in human hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Feng Zhu; Lei Jin; Tian-Ping Luo; Guang-Hua Luo; Yan Tan; Xi-Hu Qin

    2010-01-01

    BACKGROUND: HtrA1, a serine protease, is down-regulated in various human solid tumors. Overexpression of HtrA1 in human cancer cells inhibits cell growth and proliferation in vitro and in vivo, suggesting its possible role as a tumor suppressor. METHODS: Immunohistochemistry was used to determine the expression of HtrA1 in 50 hepatocellular carcinoma specimens and adjacent liver tissues. The correlation between the expression of HtrA1 and the clinico-pathologic data were analyzed. RESULTS:  The levels of HtrA1 were lower in tumor tissues than in their adjacent liver tissues. Moreover, an inverse relationship was found between HtrA1 expression and the differentiation of hepatocellular carcinoma. Loss of HtrA1 was more frequently found in tumors in Edmondson grade III-IV, especially in those with venous invasion, compared to tumors in Edmondson grade I-II. Most importantly, patients with higher HtrA1 expression had a better survival rate. CONCLUSION: All these data suggest an important role of HtrA1 in hepatocellular carcinoma development and progression, which may be a new target for its treatment.

  3. Strengths, weaknesses, opportunities and threats for HTR deployment in Europe

    Energy Technology Data Exchange (ETDEWEB)

    Bredimas, Alexandre, E-mail: alexandre.bredimas@strane-innovation.com [Strane Innovation, 27 rue Léon Bourgeois, 91120 Palaiseau (France); Kugeler, Kurt [Strane Innovation, 27 rue Léon Bourgeois, 91120 Palaiseau (France); Fütterer, Michael A. [Strane Innovation, 27 rue Léon Bourgeois, 91120 Palaiseau (France); European Commission, Joint Research Centre, Institute for Energy and Transport, P.O. Box 2, NL-1755ZG Petten (Netherlands)

    2014-05-01

    High temperature nuclear reactors are a technology, of which early versions were demonstrated in the 1960s–1980s in Germany (AVR, THTR) and the United States (Peach Bottom, Fort St. Vrain). HTRs were initially designed for high temperature, high efficiency electricity generation but the technology, the market and the targeted applications have evolved since then to address industrial cogeneration and new operational conditions (in particular new safety regulations). This paper intends to analyse the latest status of HTR today, as regards their intrinsic strengths and weaknesses and their external context, whether positive (opportunities) or negative (threats). Different dimensions are covered by the analysis: technology status, results from R and D programmes (especially in Europe), competences and skills, licensing aspects, experience feedback from demonstrator operation (in particular in Germany), economic conditions and other non-technical aspects. Europe has a comprehensive experience in the field of HTR with capabilities in both pebble bed and prismatic designs (R and D, engineering, manufacturing, operation, dismantling, and the full fuel cycle). Europe is also a promising market for HTR as the process heat market is large with good industrial and cogeneration infrastructures. The analysis of the European situation is to a good deal indicative for the global potential of this technology.

  4. Comparison of irradiation behaviour of HTR graphite grades

    Science.gov (United States)

    Heijna, M. C. R.; de Groot, S.; Vreeling, J. A.

    2017-08-01

    The INNOGRAPH irradiations were executed in the High Flux Reactor (HFR) in Petten by NRG supported by the European Framework programs HTR-M, RAPHAEL, and ARCHER to generate data on the irradiation behaviour of graphite grades for High Temperature Reactor (HTR) application available at that time. Samples of the graphite grades NBG-10, NBG-17, NBG-18, NBG-20, NBG-25, PCEA, PPEA, PCIB, and IG-110 have been irradiated at 750 °C and 950 °C. The inherent scatter induced by the probabilistic material behaviour of graphite requires uncertainty and scatter induced by test conditions and post-irradiation examination to be minimized. The INNOGRAPH irradiations supplied an adequate number of irradiated samples to enable accurate determination of material properties and their evolution under irradiation. This allows comparison of different graphite grades and a qualitative assessment of their appropriateness for HTR applications, as a basis of selection, design and core component lifetime. The results indicate that coarse grained graphite grades exhibit more favourable behaviour for application in HTRs due to their low dimensional anisotropy and fracture propagation resilience.

  5. Characterisation of worldwide Helicobacter pylori strains reveals genetic conservation and essentiality of serine protease HtrA.

    Science.gov (United States)

    Tegtmeyer, Nicole; Moodley, Yoshan; Yamaoka, Yoshio; Pernitzsch, Sandy Ramona; Schmidt, Vanessa; Traverso, Francisco Rivas; Schmidt, Thomas P; Rad, Roland; Yeoh, Khay Guan; Bow, Ho; Torres, Javier; Gerhard, Markus; Schneider, Gisbert; Wessler, Silja; Backert, Steffen

    2016-03-01

    HtrA proteases and chaperones exhibit important roles in periplasmic protein quality control and stress responses. The genetic inactivation of htrA has been described for many bacterial pathogens. However, in some cases such as the gastric pathogen Helicobacter pylori, HtrA is secreted where it cleaves the tumour-suppressor E-cadherin interfering with gastric disease development, but the generation of htrA mutants is still lacking. Here, we show that the htrA gene locus is highly conserved in worldwide strains. HtrA presence was confirmed in 992 H. pylori isolates in gastric biopsy material from infected patients. Differential RNA-sequencing (dRNA-seq) indicated that htrA is encoded in an operon with two subsequent genes, HP1020 and HP1021. Genetic mutagenesis and complementation studies revealed that HP1020 and HP1021, but not htrA, can be mutated. In addition, we demonstrate that suppression of HtrA proteolytic activity with a newly developed inhibitor is sufficient to effectively kill H. pylori, but not other bacteria. We show that Helicobacter htrA is an essential bifunctional gene with crucial intracellular and extracellular functions. Thus, we describe here the first microbe in which htrA is an indispensable gene, a situation unique in the bacterial kingdom. HtrA can therefore be considered a promising new target for anti-bacterial therapy.

  6. HtrA Is Important for Stress Resistance and Virulence in Haemophilus parasuis.

    Science.gov (United States)

    Zhang, Luhua; Li, Ying; Wen, Yiping; Lau, Gee W; Huang, Xiaobo; Wu, Rui; Yan, Qigui; Huang, Yong; Zhao, Qin; Ma, Xiaoping; Wen, Xintian; Cao, Sanjie

    2016-08-01

    Haemophilus parasuis is an opportunistic pathogen that causes Glässer's disease in swine, with polyserositis, meningitis, and arthritis. The high-temperature requirement A (HtrA)-like protease, which is involved in protein quality control, has been reported to be a virulence factor in many pathogens. In this study, we showed that HtrA of H. parasuis (HpHtrA) exhibited both chaperone and protease activities. Finally, nickel import ATP-binding protein (NikE), periplasmic dipeptide transport protein (DppA), and outer membrane protein A (OmpA) were identified as proteolytic substrates for HpHtrA. The protease activity reached its maximum at 40°C in a time-dependent manner. Disruption of the htrA gene from strain SC1401 affected tolerance to temperature stress and resistance to complement-mediated killing. Furthermore, increased autoagglutination and biofilm formation were detected in the htrA mutant. In addition, the htrA mutant was significantly attenuated in virulence in the murine model of infection. Together, these data demonstrate that HpHtrA plays an important role in the virulence of H. parasuis.

  7. DNA Methylation Analysis of HTR2A Regulatory Region in Leukocytes of Autistic Subjects.

    Science.gov (United States)

    Hranilovic, Dubravka; Blazevic, Sofia; Stefulj, Jasminka; Zill, Peter

    2016-02-01

    Disturbed brain and peripheral serotonin homeostasis is often found in subjects with autism spectrum disorder (ASD). The role of the serotonin receptor 2A (HTR2A) in the regulation of central and peripheral serotonin homeostasis, as well as its altered expression in autistic subjects, have implicated the HTR2A gene as a major candidate for the serotonin disturbance seen in autism. Several studies, yielding so far inconclusive results, have attempted to associate autism with a functional SNP -1438 G/A (rs6311) in the HTR2A promoter region, while possible contribution of epigenetic mechanisms, such as DNA methylation, to HTR2A dysregulation in autism has not yet been investigated. In this study, we compared the mean DNA methylation within the regulatory region of the HTR2A gene between autistic and control subjects. DNA methylation was analysed in peripheral blood leukocytes using bisulfite conversion and sequencing of the HTR2A region containing rs6311 polymorphism. Autistic subjects of rs6311 AG genotype displayed higher mean methylation levels within the analysed region than the corresponding controls (P < 0.05), while there was no statistically significant difference for AA and GG carriers. Our study provides preliminary evidence for increased HTR2A promoter methylation in leukocytes of a portion of adult autistic subjects, indicating that epigenetic mechanisms might contribute to HTR2A dysregulation observed in individuals with ASD.

  8. Cloning and Transcriptional Activity of the Mouse Omi/HtrA2 Gene Promoter.

    Science.gov (United States)

    Liu, Dan; Liu, Xin; Wu, Ye; Wang, Wen; Ma, Xinliang; Liu, Huirong

    2016-01-16

    HtrA serine peptidase 2 (HtrA2), also named Omi, is a pro-apoptotic protein that exhibits dramatic changes in expression levels in a variety of disorders, including ischemia/reperfusion injury, cancer, and neurodegeneration. In our study, Omi/HtrA2 protein levels were high in the heart, brain, kidney and liver, with elevated heart/brain expression in aging mice. A similar expression pattern was observed at the mRNA level, which suggests that the regulation of Omi/HtrA2 is predominately transcriptional. Promoter binding by transcription factors is the main influencing factor of transcription, and to identify specific promoter elements that contribute to the differential expression of mouse Omi/HtrA2, we constructed truncated Omi/HtrA2 promoter/luciferase reporter vectors and analyzed their relative luciferase activity; it was greatest in the promoter regions at -1205~-838 bp and -146~+93 bp, with the -838~-649 bp region exhibiting negative regulatory activity. Bioinformatics analysis suggested that the Omi/HtrA2 gene promoter contains a CpG island at -709~+37 bp, and eight heat shock transcription factor 1 (HSF1) sites, two Sp1 transcription factor (SP1)sites, one activator protein (AP) site, seven p53 sites, and four YY1 transcription factor(YY1) sites were predicted in the core areas. Furthermore, we found that p53 and HSF1 specifically binds to the Omi/HtrA2 promoter using chromatin immunoprecipitation analysis. These results provide a foundation for understanding Omi/HtrA2 regulatory mechanisms, which could further understanding of HtrA-associated diseases.

  9. HTR Spherical Super Lattice Model for Equilibrium Fuel Cycle Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Gray S. Cahng

    2005-09-01

    Advanced High Temperature gas-cooled Reactors (HTR) currently being developed (GFR, VHTR - Very High Temperature gas-cooled Reactor, PBMR, and GT-MHR) are able to achieve a simplification of safety through reliance on innovative features and passive systems. One of the innovative features in these HTRs is reliance on ceramic-coated fuel particles to retain the fission products even under extreme accident conditions. The effect of the random fuel kernel distribution in the fuel pebble / block is addressed through the use of the Dancoff correction factor in the resonance treatment. In addition, the Dancoff correction factor is a function of burnup and fuel kernel packing factor, which requires that the Dancoff correction factor be updated during Equilibrium Fuel Cycle (EqFC) analysis. Although HTR fuel is rather homogeneously dispersed in the fuel graphite matrix, the heterogeneity effects in between fuel kernels and pebbles cannot be ignored. The double-heterogeneous lattice model recently developed at the Idaho National Engineering and Environmental Laboratory (INEEL) contains tens of thousands of cubic fuel kernel cells, which makes it very difficult to deplete the fuel, kernel by kernel (KbK), for the EqFC analysis. In addition, it is not possible to preserve the cubic size and packing factor in a spherical fuel pebble. To avoid these difficulties, a newly developed and validated HTR pebble-bed Kernel-by-Kernel spherical (KbK-sph) model, has been developed and verified in this study. The objective of this research is to introduce the KbK-sph model and super whole Pebble lattice model (PLM). The verified double-heterogeneous KbK-sph and pebble homogeneous lattice model (HLM) are used for the fuel burnup chracteristics analysis and important safety parameters validation. This study summarizes and compares the KbK-sph and HLM burnup analyzed results. Finally, we discus the Monte-Carlo coupling with a fuel depletion and buildup code - Origen-2 as a fuel burnup

  10. Technique distribuee de gestion de la charge sur le reseau electrique et ring-tree: Un nouveau systeme de communication P2P

    Science.gov (United States)

    Ayoub, Simon

    Le reseau de distribution et de transport de l'electricite se modernise dans plusieurs pays dont le Canada. La nouvelle generation de ce reseau que l'on appelle smart grid, permet entre autre l'automatisation de la production, de la distribution et de la gestion de la charge chez les clients. D'un autre cote, des appareils domestiques intelligents munis d'une interface de communication pour des applications du smart grid commencent a apparaitre sur le marche. Ces appareils intelligents pourraient creer une communaute virtuelle pour optimiser leurs consommations d'une facon distribuee. La gestion distribuee de ces charges intelligentes necessite la communication entre un grand nombre d'equipements electriques. Ceci represente un defi important a relever surtout si on ne veut pas augmenter le cout de l'infrastructure et de la maintenance. Lors de cette these deux systemes distincts ont ete concus : un systeme de communication peer-to-peer, appele Ring-Tree, permettant la communication entre un nombre important de noeuds (jusqu'a de l'ordre de grandeur du million) tel que des appareils electriques communicants et une technique distribuee de gestion de la charge sur le reseau electrique. Le systeme de communication Ring-Tree inclut une nouvelle topologie reseau qui n'a jamais ete definie ou exploitee auparavant. Il inclut egalement des algorithmes pour la creation, l'exploitation et la maintenance de ce reseau. Il est suffisamment simple pour etre mis en oeuvre sur des controleurs associes aux dispositifs tels que des chauffe-eaux, chauffage a accumulation, bornes de recharges electriques, etc. Il n'utilise pas un serveur centralise (ou tres peu, seulement lorsqu'un noeud veut rejoindre le reseau). Il offre une solution distribuee qui peut etre mise en oeuvre sans deploiement d'une infrastructure autre que les controleurs sur les dispositifs vises. Finalement, un temps de reponse de quelques secondes pour atteindre 1'ensemble du reseau peut etre obtenu, ce qui est

  11. First results for fluid dynamics, neutronics and fission product behavior in HTR applying the HTR code package (HCP) prototype

    Energy Technology Data Exchange (ETDEWEB)

    Allelein, H.-J., E-mail: h.j.allelein@fz-juelich.de [Forschungszentrum Jülich, 52425 Jülich (Germany); Institute for Reactor Safety and Reactor Technology, RWTH Aachen University, 52064 Aachen (Germany); Kasselmann, S.; Xhonneux, A.; Tantillo, F.; Trabadela, A.; Lambertz, D. [Forschungszentrum Jülich, 52425 Jülich (Germany)

    2016-09-15

    To simulate the different aspects of High Temperature Reactor (HTR) cores, a variety of specialized computer codes have been developed at Forschungszentrum Jülich (IEK-6) and Aachen University (LRST) in the last decades. In order to preserve knowledge, to overcome present limitations and to make these codes applicable to modern computer clusters, these individual programs are being integrated into a consistent code package. The so-called HTR code package (HCP) couples the related and recently applied physics models in a highly integrated manner and therefore allows to simulate phenomena with higher precision in space and time while at the same time applying state-of-the-art programming techniques and standards. This paper provides an overview of the status of the HCP and reports about first benchmark results for an HCP prototype which couples the fluid dynamics and time dependent neutronics code MGT-3D, the burn up code TNT and the fission product release code STACY. Due to the coupling of MGT-3D and TNT, a first step towards a new reactor operation and accident simulation code was made, where nuclide concentrations calculated by TNT lead to new cross sections, which are fed back into MGT-3D. Selected operation scenarios of the HTR-Module 200 concept plant and the HTTR were chosen to be simulated with the HCP prototype. The fission product release during normal operation conditions will be calculated with STACY based on a core status derived from SERPENT and MGT-3D. Comparisons will be shown against data generated by SERPENT and the legacy codes VSOP99/11, NAKURE and FRESCO-II.

  12. Improved Neutronics Treatment of Burnable Poisons for the Prismatic HTR

    Energy Technology Data Exchange (ETDEWEB)

    Y. Wang; A. A. Bingham; J. Ortensi; C. J. Permann

    2012-10-01

    In prismatic block High Temperature Reactors (HTR), highly absorbing material such a burnable poison (BP) cause local flux depressions and large gradients in the flux across the blocks which can be a challenge to capture accurately with traditional homogenization methods. The purpose of this paper is to quantify the error associated with spatial homogenization, spectral condensation and discretization and to highlight what is needed for improved neutronics treatments of burnable poisons for the prismatic HTR. A new triangular based mesh is designed to separate the BP regions from the fuel assembly. A set of packages including Serpent (Monte Carlo), Xuthos (1storder Sn), Pronghorn (diffusion), INSTANT (Pn) and RattleSnake (2ndorder Sn) is used for this study. The results from the deterministic calculations show that the cross sections generated directly in Serpent are not sufficient to accurately reproduce the reference Monte Carlo solution in all cases. The BP treatment produces good results, but this is mainly due to error cancellation. However, the Super Cell (SC) approach yields cross sections that are consistent with cross sections prepared on an “exact” full core calculation. In addition, very good agreement exists between the various deterministic transport and diffusion codes in both eigenvalue and power distributions. Future research will focus on improving the cross sections and quantifying the error cancellation.

  13. Nonuniform Oxidation on the Surface of Fuel Element in HTR

    Directory of Open Access Journals (Sweden)

    Peng Liu

    2016-01-01

    Full Text Available The graphite oxidation of fuel element has obtained high attention in air ingress accident analysis of high temperature gas-cooled reactor (HTR. The shape function, defined as the relationship between the maximum and the average of the oxidation, is an important factor to estimate the consequence of the accident. There are no detailed studies on the shape function currently except two experiments several decades ago. With the development of computer technology, CFD method is used in the numerical experiment about graphite oxidation in pebble bed of HTR in this paper. Structured packed beds are used in the calculation instead of random packed beds. The result shows the nonuniform distribution of oxidation on the sphere surface and the shape function in the condition of air ingress accident. Furthermore, the sensitive factors of shape function, such as temperature and Re number, are discussed in detail and the relationship between the shape function and sensitive factors is explained. According to the results in this paper, the shape function ranges from 1.05 to 4.7 under the condition of temperature varying from 600°C to 1200°C and Re varying from 16 to 1600.

  14. The developmental basis of epigenetic regulation of HTR2A and psychiatric outcomes.

    Science.gov (United States)

    Paquette, Alison G; Marsit, Carmen J

    2014-12-01

    The serotonin receptor 5-HT2A (encoded by HTR2A) is an important regulator of fetal brain development and adult cognitive function. Environmental signals that induce epigenetic changes of serotonin response genes, including HTR2A, have been implicated in adverse mental health outcomes. The objective of this perspective article is to address the medical implications of HTR2A epigenetic regulation, which has been associated with both infant neurobehavioral outcomes and adult mental health. Ongoing research has identified a region of the HTR2A promoter that has been associated with a number of medical outcomes in adults and infants, including bipolar disorder, schizophrenia, chronic fatigue syndrome, borderline personality disorder, suicidality, and neurobehavioral outcomes. Epigenetic regulation of HTR2A has been studied in several different types of tissues, including the placenta. The placenta is an important source of serotonin during fetal neurodevelopment, and placental epigenetic variation of HTR2A has been associated with infant neurobehavioral outcomes, which may represent the basis of adult mental health disorders. Further analysis is needed to identify intrinsic and extrinsic factors that modulate HTR2A methylation, and the mechanism by which this epigenetic variation influences fetal growth and leads to altered brain development, manifesting in psychiatric disorders.

  15. The nucleus accumbens 5-HTR4-CART pathway ties anorexia to hyperactivity

    Science.gov (United States)

    Jean, A; Laurent, L; Bockaert, J; Charnay, Y; Dusticier, N; Nieoullon, A; Barrot, M; Neve, R; Compan, V

    2012-01-01

    In mental diseases, the brain does not systematically adjust motor activity to feeding. Probably, the most outlined example is the association between hyperactivity and anorexia in Anorexia nervosa. The neural underpinnings of this ‘paradox', however, are poorly elucidated. Although anorexia and hyperactivity prevail over self-preservation, both symptoms rarely exist independently, suggesting commonalities in neural pathways, most likely in the reward system. We previously discovered an addictive molecular facet of anorexia, involving production, in the nucleus accumbens (NAc), of the same transcripts stimulated in response to cocaine and amphetamine (CART) upon stimulation of the 5-HT4 receptors (5-HTR4) or MDMA (ecstasy). Here, we tested whether this pathway predisposes not only to anorexia but also to hyperactivity. Following food restriction, mice are expected to overeat. However, selecting hyperactive and addiction-related animal models, we observed that mice lacking 5-HTR1B self-imposed food restriction after deprivation and still displayed anorexia and hyperactivity after ecstasy. Decryption of the mechanisms showed a gain-of-function of 5-HTR4 in the absence of 5-HTR1B, associated with CART surplus in the NAc and not in other brain areas. NAc-5-HTR4 overexpression upregulated NAc-CART, provoked anorexia and hyperactivity. NAc-5-HTR4 knockdown or blockade reduced ecstasy-induced hyperactivity. Finally, NAc-CART knockdown suppressed hyperactivity upon stimulation of the NAc-5-HTR4. Additionally, inactivating NAc-5-HTR4 suppressed ecstasy's preference, strengthening the rewarding facet of anorexia. In conclusion, the NAc-5-HTR4/CART pathway establishes a ‘tight-junction' between anorexia and hyperactivity, suggesting the existence of a primary functional unit susceptible to limit overeating associated with resting following homeostasis rules. PMID:23233022

  16. Neutronic feasibility design of a small long-life HTR

    Energy Technology Data Exchange (ETDEWEB)

    Ding Ming, E-mail: m.ding@tudelft.nl [Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands); Kloosterman, Jan Leen, E-mail: J.L.Kloosterman@tudelft.nl [Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands)

    2011-12-15

    Highlights: Black-Right-Pointing-Pointer We propose the neutronic feasibility design of a small, long lifetime and transportable HTR. Black-Right-Pointing-Pointer Comparison of cylindrical, annular and scatter cores of the small block-type HTR. Black-Right-Pointing-Pointer The design of the scatter core effectively reduces the number of the fuel block and increases the lifetime and burnup of the reactor. - Abstract: Small high temperature gas-cooled reactors (HTRs) have the advantages of transportability, modular construction and flexible site selection. This paper presents the neutronic feasibility design of a 20 MWth U-Battery, which is a long-life block-type HTR. Key design parameters and possible reactor core configurations of the U-Battery were investigated by SCALE 5.1. The design parameters analyzed include fuel enrichment, the packing fraction of TRISO particles, the radii of fuel compacts and kernels, and the thicknesses of top and bottom reflectors. Possible reactor core configurations investigated include five cylindrical, two annular and four scatter reactor cores for the U-Battery. The neutronic design shows that the 20 MWth U-Battery with a 10-year lifetime is feasible using less than 20% enriched uranium, while the negative values of the temperature coefficients of reactivity partly ensure the inherent safety of the U-Battery. The higher the fuel enrichment and the packing fraction of TRISO particles are, the lower the reactivity swing during 10 years will be. There is an optimum radius of fuel kernels for each value of the fuel compact design parameter (i.e., radius) and a specific fuel lifetime. Moreover, the radius of fuel kernels has a small influence on the infinite multiplication factor of a typical fuel block in the range of 0.2-0.25 mm, when the radius of fuel compacts is 0.6225 cm and the lifetime of the fuel block is 10 years. The comparison of the cylindrical reactor cores with the non-cylindrical ones shows that neutron under

  17. HTR-PM反应堆保护系统工程样机的研制%Development of HTR-PM reactor protection system engineering prototype

    Institute of Scientific and Technical Information of China (English)

    李铎; 熊华胜; 郭超; 张良驹; 石铭德

    2013-01-01

    High Temperature Gas-Cooled Reactor-Pebble bed Module (HTR-PM) Nuclear Power Plant (NPP) is one of the most important projects in the National Science&Technology Major Project during the Eleventh Five-Year Plan period.HTR-PM digital Reactor Protection System (RPS) is one of the key research subjects in the HTR-PM NPP supported by the National S&T Major Project. During the development of HTR-PM RPS engineering prototype,the safety software life cycle model is V model which includes such steps as computer system architecture design,software requirements,software design,software implementation and computer system integration.In paral el with safety software development steps there are software Verification and Validation (V&V) processes,which include special actions in each process to verify and validate the software development steps.HTR-PM RPS engineering prototype is ful y made in China and provides HTR-PM NPP correlative technologies with al intel ectual property rights,which achieve the final goal of the National S&T Major Project.%高温气冷堆核电站(HTR-PM)是国家“十一·五”重大专项支持的重点工程,数字化保护系统是HTR-PM重大专项关键技术及相关试验研究项目之一。HTR-PM保护系统工程样机的安全软件生命周期模型选择了典型的“V”字模型,样机研制过程中包括计算机系统设计、软件需求、软件设计、软件实现和计算机系统集成等阶段。与安全软件开发相应的是安全软件的验证与确认,对应每个开发步骤有相应的软件验证与确认活动,以保证安全软件开发过程的质量。保护系统工程样机的成功研制将形成拥有自主知识产权的高温气冷堆核电站配套技术。

  18. Evaluation of Borrelia burgdorferi BbHtrA Protease as a Vaccine Candidate for Lyme Borreliosis in Mice.

    Directory of Open Access Journals (Sweden)

    Amy J Ullmann

    Full Text Available Borrelia burgdorferi synthesizes an HtrA protease (BbHtrA which is a surface-exposed, conserved protein within Lyme disease spirochetes with activity toward CheX and BmpD of Borrelia spp, as well as aggrecan, fibronectin and proteoglycans found in skin, joints and neural tissues of vertebrates. An antibody response against BbHtrA is observed in Lyme disease patients and in experimentally infected laboratory mice and rabbits. Given the surface location of BbHtrA on B. burgdorferi and its ability to elicit an antibody response in infected hosts, we explored recombinant BbHtrA as a potential vaccine candidate in a mouse model of tick-transmitted Lyme disease. We immunized mice with two forms of BbHtrA: the proteolytically active native form and BbHtrA ablated of activity by a serine to alanine mutation at amino acid 226 (BbHtrA(S226A. Although inoculation with either BbHtrA or BbHtrA(S226A produced high-titer antibody responses in C3H/HeJ mice, neither antigen was successful in protecting mice from B. burgdorferi challenge. These results indicate that the search for novel vaccine candidates against Lyme borreliosis remains a challenge.

  19. The novel role of HtrA1 in gingivitis, chronic and aggressive periodontitis.

    Science.gov (United States)

    Lorenzi, Teresa; Niţulescu, Elena Annabel; Zizzi, Antonio; Lorenzi, Maria; Paolinelli, Francesca; Aspriello, Simone Domenico; Baniţă, Monica; Crăiţoiu, Stefania; Goteri, Gaia; Barbatelli, Giorgio; Lombardi, Tommaso; Di Felice, Roberto; Marzioni, Daniela; Rubini, Corrado; Castellucci, Mario

    2014-01-01

    Proteolytic tissue degradation is a typical phenomenon in inflammatory periodontal diseases. HtrA1 (High temperature requirement A 1) has a serine protease activity and is able to degrade fibronectin whose fragments induce the expression and secretion of several matrix metalloproteinases (MMPs). The aim of this study was to investigate for the first time if HtrA1 has a role in gingivitis and in generalized forms of chronic and aggressive periodontitis. Expression of HtrA1 was investigated in 16 clinically healthy gingiva, 16 gingivitis, 14 generalized chronic periodontitis and 10 generalized aggressive periodontitis by immunohistochemistry and real-time PCR. Statistical comparisons were performed by the Kruskall-Wallis test. Significantly higher levels of HtrA1 mRNA and protein expression were observed in pathological respect to healthy tissues. In particular, we detected an increase of plasma cell HtrA1 immunostaining from gingivitis to chronic and aggressive periodontitis, with the higher intensity in aggressive disease. In addition, we observed the presence of HtrA1 in normal and pathological epithelium, with an increased expression, particularly in its superficial layer, associated with increasingly severe forms of periodontal disease. We can affirm that HtrA1 expression in plasma cells could be correlated with the destruction of pathological periodontal tissue, probably due to its ability to trigger the overproduction of MMPs and to increase the inflammatory mediators TNF-α and IL-1β by inhibition of TGF-β. Moreover, epithelial HtrA1 immunostaining suggests a participation of the molecule in the host inflammatory immune responses necessary for the control of periodontal infection.

  20. Reseau Environnement's brief on the project regarding atmospheric regulations : submitted to the Minister of Sustainable Development, Environment and Parks; Memoire de Reseau Environnement sur le projet de reglement sur l'assainissement de l'atmosphere : transmis au ministere du Developpement durable, de l'Environnement et des Parcs

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-01-13

    Reseau Environnement is a Montreal-based organization that promotes the protection of ecosystems and human health. Their mandate is to extend the existing standards for reducing pollutants and to tap the full potential of Quebec expertise in addressing pollution sources. Reseau Environnement recently appealed to the Quebec Minister of Sustainable Development, Environment and Parks to develop clear, flexible and modern regulations for Quebec, similar to those found in Europe and the United States, to efficiently control atmospheric emissions in an effort to counteract the negative effects they impart on ecosystems and human health. Among the requests was the revision of certain pollution regulation clauses to regulate odor emissions; identify preferred measuring methods for pollutants; apply ambient air quality standards to existing installations; apply standards for particulates; impose requirements for the frequency of pollution sampling and make changes to some components of Montreal's Regulation 90 regarding air pollution from industrial activities. 13 refs.

  1. Different contributions of HtrA protease and chaperone activities to Campylobacter jejuni stress tolerance and physiology

    DEFF Research Database (Denmark)

    Bæk, Kristoffer Torbjørn; Vegge, Christina Skovgaard; Skórko-Glonek, Joanna;

    2011-01-01

    The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope such as HtrA and SurA. HtrA displays both chaperone and protease activity......, but little is known about how each of these activities contributes to stress tolerance in bacteria. In vitro experiments showed temperature dependent protease and chaperone activities of C. jejuni HtrA. A C. jejuni mutant lacking only the protease activity of HtrA was used to show that the HtrA chaperone...... activity is sufficient for growth at high temperature or oxidative stress, whereas the HtrA protease activity is only essential at conditions close to the growth limit for C. jejuni. However, the protease activity was required to prevent induction of the cytoplasmic heat-shock response even at optimal...

  2. Evidence of a conserved role for Chlamydia HtrA in the replication phase of the chlamydial developmental cycle.

    Science.gov (United States)

    Patel, Pooja; De Boer, Leonore; Timms, Peter; Huston, Wilhelmina May

    2014-08-01

    Identification of the HtrA inhibitor JO146 previously enabled us to demonstrate an essential function for HtrA during the mid-replicative phase of the Chlamydia trachomatis developmental cycle. Here we extend our investigations to other members of the Chlamydia genus. C. trachomatis isolates with distinct replicative phase growth kinetics showed significant loss of viable infectious progeny after HtrA was inhibited during the replicative phase. Mid-replicative phase addition of JO146 was also significantly detrimental to Chlamydia pecorum, Chlamydia suis and Chlamydia cavie. These data combined indicate that HtrA has a conserved critical role during the replicative phase of the chlamydial developmental cycle.

  3. CEA and AREVA HTR fuel particles manufacturing and characterization R and D Program

    Energy Technology Data Exchange (ETDEWEB)

    Charollais, F.; Perez, M.; Fonquernie, S.; Ablitzer, C.; Duhart, A.; Perrais, C.; Dugne, O.; Guillermier, P.; Harbonnier, G

    2004-07-01

    Worldwide, renewal of interest for High Temperature Reactors (HTR) moderated by graphite, using helium gas as coolant is due to the possibility offered by HTR technology to build small competitive modular units (100-300 MWe) and to produce high temperature gas required for hydrogen production or heat production. In this context, Commissariat a l'Energie Atomique (CEA) supported by AREVA, conducts R and D projects on HTR, which one is the mastering of the UO{sub 2} TRISO fuel fabrication technology including innovating prospects. The first step of the work is to recover fabrication know-how and to improve fabrication processes (for preparing the erection of a fuel pilot manufacture line of HTR particles). The second step will be the manufacturing of HTR fuel elements in purpose of irradiation tests. This publication deals with the know-how recovery and improved processes of UO{sub 2} TRISO manufacture, i.e. kernel and coatings manufacturing, the development of innovative characterization methods, a modelling approach of CVD processes and the status of the future laboratory manufacture line. Alternative fuel designs are also investigated, for instance the way of manufacturing UCO and UC{sub x} kernels with higher refractory coating material such as ZrC. (authors)

  4. Le marché des armes après la guerre froide et les pays les moins développés

    OpenAIRE

    2002-01-01

    Les projets pour limiter le commerce des armes se sont multipliés avee l'achèvement d'un bon nombre de contlits régionaux et la fin de la guerre froide. La guerre du Golfe a également fait évoluer les conditions de sécurité des pays les moins développés, et les principaux vendeurs d'armes ont diminué leurs aides économiques à l'exportation des armes. A partir de l'examen de nombreux cas régionaux, l'auteur conclut que l'avenir des politiques d'armement des pays sous-développés dépend de l'ord...

  5. Histone acetylation of the htr3a gene in the prefrontal cortex of Wistar rats regulates ethanol-seeking behavior

    Institute of Scientific and Technical Information of China (English)

    Yahui Xu; Xuebing Liu; Xiaojie Zhang; Guanbai Zhang; Ruiling Zhang; Tieqiao Liu; Wei Hao

    2012-01-01

    Previous reports showed that decreased histone deacetylase activity significantly potentiated the rewarding effects of psychostimulants, and that encoding of the 5-HT3 receptor by the htr3a gene was related to ethanol-seeking behavior. However, the effects of a histone deacetylase inhibitor on ethanol-seeking behavior and epigenetic regulation of htr3a mRNA expression after chronic ethanol exposure are not fully understood. Using quantitative reverse transcription-polymerase chain reaction and chromatin immunoprecipitation analysis, we investigated the effects of chronic ethanol exposure and its interaction with a histone deacetylase inhibitor on histone-acetylation-mediated changes in htr3a mRNA expression in the htr3a promoter region. The conditioned place preference procedure was used to evaluate ethanol-seeking behavior. Chronic exposure to ethanol effectively elicited place conditioning. In the prefrontal cortex, the acetylation of H3K9 and htr3a mRNA expression in the htr3a promoter region were significantly higher in the ethanol group than in the saline group. The histone deacetylase inhibitor sodium butyrate potentiated the effects of ethanol on htr3a mRNA expression and enhanced ethanol-induced conditioned place preferences. These results suggest that ethanol upregulates htr3a levels through mechanisms involving H3K9 acetylation, and that histone acetylation may be a therapeutic target for treating ethanol abuse.

  6. Research on graphite powders used for HTR-PM fuel elements

    Institute of Scientific and Technical Information of China (English)

    ZHAO Hongsheng; LIANG Tongxiang; ZHANG Jie; LI Ziqiang; TANG Chunhe

    2006-01-01

    Different batches of natural graphite powders and electrographite powders were characterized by impurity, degree of graphitization, particle size distribution, specific surface area, and shape characteristics. The graphite balls consist of proper mix-ratio of natural graphite, electrographite and phenolic resin were manufactured and characterized by thermal conductivity, anisotropy of thermal expansion, crush strength, and drop strength. Results show that some types of graphite powders possess very high purity, degree of graphitization, and sound size distribution and apparent density, which can serve for matrix graphite of HTR-PM. The graphite balls manufactured with reasonable mix-ratio of graphite powders and process method show very good properties. It is indicated that the properties of graphite balls can meet the design criterion of HTR-PM. We can provide a powerful candidate material for the future manufacture of HTR-PM fuel elements.

  7. Utilization of HTR reflector graphite as embedding matrix for radioactive waste

    Energy Technology Data Exchange (ETDEWEB)

    Fachinger, J., E-mail: fachinger@fnag.eu [Furnaces Nuclear Applications Grenoble, Wilhelm-Rohn-Strasse 35, 63450 Hanau (Germany); Grosse, K.H. [Furnaces Nuclear Applications Grenoble, Wilhelm-Rohn-Strasse 35, 63450 Hanau (Germany); Hrovat, M.; Seemann, R. [ALD, Wilhelm-Rohn-Strasse 35, 63450 Hanau (Germany)

    2012-10-15

    The reflector graphite of an HTR reactor has to be handled as radioactive waste after the operational period of the reactor. However the waste management of irradiated graphite from Magnox reactors shows, that waste management of even low contaminated graphite could be expensive and requires special retrieval, treatment and disposal technologies for safe long term storage as low or medium radioactive waste. However the reflector graphite could be transferred into long term stable embedding matrix for high level radioactive waste especially for HTR fuel elements. This can be achieved by closing the pore system of the graphite with a stable inorganic binder, e.g. glass. First investigations proved the sealing of the pore system and the potential for embedding HTR fuel pebbles.

  8. The functional -1019C/G HTR1A polymorphism and mechanisms of fear.

    Science.gov (United States)

    Straube, B; Reif, A; Richter, J; Lueken, U; Weber, H; Arolt, V; Jansen, A; Zwanzger, P; Domschke, K; Pauli, P; Konrad, C; Gerlach, A L; Lang, T; Fydrich, T; Alpers, G W; Ströhle, A; Wittmann, A; Pfleiderer, B; Wittchen, H-U; Hamm, A; Deckert, J; Kircher, T

    2014-12-16

    Serotonin receptor 1A gene (HTR1A) knockout mice show pronounced defensive behaviour and increased fear conditioning to ambiguous conditioned stimuli. Such behaviour is a hallmark of pathological human anxiety, as observed in panic disorder with agoraphobia (PD/AG). Thus, variations in HTR1A might contribute to neurophysiological differences within subgroups of PD/AG patients. Here, we tested this hypothesis by combining genetic with behavioural techniques and neuroimaging. In a clinical multicentre trial, patients with PD/AG received 12 sessions of manualized cognitive-behavioural therapy (CBT) and were genotyped for HTR1A rs6295. In four subsamples of this multicentre trial, exposure behaviour (n=185), defensive reactivity measured using a behavioural avoidance test (BAT; before CBT: n=245; after CBT: n=171) and functional magnetic resonance imaging (fMRI) data during fear conditioning were acquired before and after CBT (n=39). HTR1A risk genotype (GG) carriers more often escaped during the BAT before treatment. Exploratory fMRI results suggest increased activation of the amygdala in response to threat as well as safety cues before and after treatment in GG carriers. Furthermore, GG carriers demonstrated reduced effects of CBT on differential conditioning in regions including the bilateral insulae and the anterior cingulate cortex. Finally, risk genotype carriers demonstrated reduced self-initiated exposure behaviour to aversive situations. This study demonstrates the effect of HTR1A variation on defensive behaviour, amygdala activity, CBT-induced neural plasticity and normalization of defence behaviour in PD/AG. Our results, therefore, translate evidence from animal studies to humans and suggest a central role for HTR1A in differentiating subgroups of patients with anxiety disorders.

  9. The association between HTR2C gene polymorphisms and the metabolic syndrome in patients with schizophrenia.

    Science.gov (United States)

    Mulder, Hans; Franke, Barbara; van der-Beek van der, Annemarie Aart; Arends, Johan; Wilmink, Frederik W; Scheffer, Hans; Egberts, Antoine C G

    2007-08-01

    The use of antipsychotics is associated with metabolic side effects, which put patients with schizophrenia or related disorders at risk for cardiovascular morbidity. The high interindividual variability in antipsychotic-induced metabolic abnormalities suggests that genetic makeup is a possible determinant. In this cross-sectional study, we investigated whether genotypes of the HTR2C receptor are associated with the metabolic syndrome in patients using antipsychotics. Patients were identified from a schizophrenia disease management program. In this program, patients' blood pressure, triglycerides, high-density lipoprotein-cholesterol, and waist circumference are measured regularly during follow-up. The primary end point of our study was the prevalence of the metabolic syndrome as classified by a modified version of the National Cholesterol Education Program's Adult Treatment Panel III. Primary determinants were polymorphisms in the HTR2C receptor gene (HTR2C:c.1-142948[GT]n, rs3813928 [-997 G/A], rs3813929 [-759 C/T], rs518147 [-697 G/C], and rs1414334 [C > G]). The included patients (n = 112) mainly (>80%) used atypical antipsychotics (clozapine, olanzapine, and risperidone). Carriership of the variant alleles of the HTR2C polymorphisms rs518147, rs1414334, and HTR2C:c.1-142948(GT)n was associated with an increased risk of the metabolic syndrome (adjusted odds ratio [OR], 2.62 [95% confidence interval {CI}, 1.00-6.85]; OR, 4.09 [95% CI, 1.41-11.89]; and OR, 3.12 [95% CI, 1.13-8.16]), respectively. Our findings suggest that HTR2C genotypes are associated with antincreased risk of metabolic syndrome in patients taking antipsychotics.

  10. Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT3 Antagonist Granisetron

    Science.gov (United States)

    Hedenberg-Magnusson, Britt; List, Thomas; Svensson, Peter; Schalling, Martin

    2016-01-01

    The aim of this study was to investigate experimentally if 5-HT3 single nucleotide polymorphisms (SNP) contribute to pain perception and efficacy of the 5-HT3-antagonist granisetron and sex differences. Sixty healthy participants were genotyped regarding HTR3A (rs1062613) and HTR3B (rs1176744). First, pain was induced by bilateral hypertonic saline injections (HS, 5.5%, 0.2 mL) into the masseter muscles. Thirty min later the masseter muscle on one side was pretreated with 0.5 mL granisetron (1 mg/mL) and on the other side with 0.5 mL placebo (isotonic saline) followed by another HS injection (0.2 mL). Pain intensity, pain duration, pain area and pressure pain thresholds (PPTs) were assessed after each injection. HS evoked moderate pain, with higher intensity in the women (P = 0.023), but had no effect on PPTs. None of the SNPs influenced any pain variable in general, but compared to men, the pain area was larger in women carrying the C/C (HTR3A) (P = 0.015) and pain intensity higher in women with the A/C alleles (HTR3B) (P = 0.019). Pre-treatment with granisetron reduced pain intensity, duration and area to a lesser degree in women (P < 0.05), but the SNPs did not in general influence the efficacy of granisetron. Women carrying the C/T & T/T (HTR3A) genotype had less reduction of pain intensity (P = 0.041) and area (P = 0.005), and women with the C/C genotype (HTR3B) had less reduction of pain intensity (P = 0.030), duration (P = 0.030) and area compared to men (P = 0.017). In conclusion, SNPs did not influence experimental muscle pain or the effect of granisetron on pain variables in general, but there were some sex differences in pain variables that seem to be influenced by genotypes. However, due to the small sample size further research is needed before any firm conclusions can be drawn. PMID:28002447

  11. Recombinant outer membrane vesicles carrying Chlamydia muridarum HtrA induce antibodies that neutralize chlamydial infection in vitro

    Directory of Open Access Journals (Sweden)

    Erika Bartolini

    2013-05-01

    Full Text Available Background: Outer membrane vesicles (OMVs are spheroid particles released by all Gram-negative bacteria as a result of the budding out of the outer membrane. Since they carry many of the bacterial surface-associated proteins and feature a potent built-in adjuvanticity, OMVs are being utilized as vaccines, some of which commercially available. Recently, methods for manipulating the protein content of OMVs have been proposed, thus making OMVs a promising platform for recombinant, multivalent vaccines development. Methods: Chlamydia muridarum DO serine protease HtrA, an antigen which stimulates strong humoral and cellular responses in mice and humans, was expressed in Escherichia coli fused to the OmpA leader sequence to deliver it to the OMV compartment. Purified OMVs carrying HtrA (CM rHtrA-OMV were analyzed for their capacity to induce antibodies capable of neutralizing Chlamydia infection of LLC-MK2 cells in vitro. Results: CM rHtrA-OMV immunization in mice induced antibodies that neutralize Chlamydial invasion as judged by an in vitro infectivity assay. This was remarkably different from what observed with an enzymatically functional recombinant HtrA expressed in, and purified from the E. coli cytoplasm (CM rHtrA. The difference in functionality between anti-CM rHtrA and anti-CM rHtrA-OMV antibodies was associated to a different pattern of protein epitopes recognition. The epitope recognition profile of anti-CM HtrA-OMV antibodies was similar to that induced in mice during Chlamydial infection. Conclusions: When expressed in OMVs HtrA appears to assume a conformation similar to the native one and this results in the elicitation of functional immune responses. These data further support the potentiality of OMVs as vaccine platform.

  12. Vers une décentralisation de la gestion de l’eau froide en habitat collectif : la question de la généralisation du comptage individuel à Paris

    Directory of Open Access Journals (Sweden)

    Agathe Euzen

    2004-10-01

    Full Text Available La gestion de l’eau froide en habitat collectif tend à évoluer en France étant donné la nouvelle réglementation en vigueur. Mieux maîtriser l’eau, responsabiliser les usagers de leurs consommations, assurer une plus grande équité… sont les arguments avancés pour favoriser la généralisation du comptage individuel. Cependant, la décentralisation de la gestion de l’eau froide en habitat collectif génère de nombreuses questions d’ordre technique, économique et social et ouvre de nouvelles réflexions sur la circulation de l’eau dans un immeuble et l’évolution des relations entre les différents acteurs.

  13. Knockdown of Litopenaeus vannamei HtrA2, an up-regulated gene in response to WSSV infection, leading to delayed shrimp mortality.

    Science.gov (United States)

    Peepim, Termsri; Phiwsaiya, Kornsunee; Charoensapsri, Walaiporn; Khunrae, Pongsak; Senapin, Saengchan; Rattanarojpong, Triwit

    2016-02-10

    HtrA2 is an apoptosis-activating gene that enhances the apoptotic process by preventing the formation of the IAP-caspase complex, thereby freeing caspase to trigger the apoptosis pathway. In this study, we presented the full-length cDNA sequence of HtrA2 from Litopenaeus vannamei (LvHtrA2). The full-length LvHtrA2 was 1335 bp, encoding 444 amino acids. This deduced amino acid sequence contained five conserved domains: a mitochondrial targeting signal (MTS), a transmembrane (TM) domain, an IAP-binding motif (IBM), a trimerization motif, a serine protease domain, and a PDZ domain normally found in the HtrA2 proteins of other organisms. A phylogenetic analysis revealed that LvHtrA2 clustered with the HtrA2 from other invertebrates and was closely related to Penaeus monodon HtrA2 (PmHtrA2). RT-PCR with RNA extracts from L. vannamei revealed that LvHtrA2 expression was found in several tissues, including the lymphoid organs, the haemocytes, the hepatopancreas, the gill, and the stomach, with different expression levels. When determining the role of LvHtrA2 in WSSV infection, it was found that LvHtrA2 transcription was early up-regulated in the WSSV-infected shrimp at 8h post-infection (p.i.) and expression still remained high at 48 h p.i.. It also demonstrated that dsRNA specific to LvHtrA2 reduced the cumulative mortality in the WSSV-infected shrimp compared with the control group. Additionally, depletion of the LvHtrA2 transcripts reduced expression levels for caspase-3 (Cap-3) gene in shrimp. This result could suggest that LvHtrA2 may involved in apoptosis mediated mortality rather than providing immune protection during WSSV infection.

  14. Development of a Reliable Fuel Depletion Methodology for the HTR-10 Spent Fuel Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Kiwhan [Los Alamos National Laboratory; Beddingfield, David H. [Los Alamos National Laboratory; Geist, William H. [Los Alamos National Laboratory; Lee, Sang-Yoon [unaffiliated

    2012-07-03

    A technical working group formed in 2007 between NNSA and CAEA to develop a reliable fuel depletion method for HTR-10 based on MCNPX and to analyze the isotopic inventory and radiation source terms of the HTR-10 spent fuel. Conclusions of this presentation are: (1) Established a fuel depletion methodology and demonstrated its safeguards application; (2) Proliferation resistant at high discharge burnup ({approx}80 GWD/MtHM) - Unfavorable isotopics, high number of pebbles needed, harder to reprocess pebbles; (3) SF should remain under safeguards comparable to that of LWR; and (4) Diversion scenarios not considered, but can be performed.

  15. Chlamydia trachomatis responds to heat shock, penicillin induced persistence, and IFN-gamma persistence by altering levels of the extracytoplasmic stress response protease HtrA

    Directory of Open Access Journals (Sweden)

    Mathews Sarah A

    2008-11-01

    Full Text Available Abstract Background Chlamydia trachomatis, an obligate intracellular human pathogen, is the most prevalent bacterial sexually transmitted infection worldwide and a leading cause of preventable blindness. HtrA is a virulence and stress response periplasmic serine protease and molecular chaperone found in many bacteria. Recombinant purified C. trachomatis HtrA has been previously shown to have both activities. This investigation examined the physiological role of Chlamydia trachomatis HtrA. Results The Chlamydia trachomatis htrA gene complemented the lethal high temperature phenotype of Escherichia coli htrA- (>42°C. HtrA levels were detected to increase by western blot and immunofluorescence during Chlamydia heat shock experiments. Confocal laser scanning microscopy revealed a likely periplasmic localisation of HtrA. During penicillin induced persistence of Chlamydia trachomatis, HtrA levels (as a ratio of LPS were initially less than control acute cultures (20 h post infection but increased to more than acute cultures at 44 h post infection. This was unlike IFN-γ persistence where lower levels of HtrA were observed, suggesting Chlamydia trachomatis IFN-γ persistence does not involve a broad stress response. Conclusion The heterologous heat shock protection for Escherichia coli, and increased HtrA during cell wall disruption via penicillin and heat shock, indicates an important role for HtrA during high protein stress conditions for Chlamydia trachomatis.

  16. The "RESEAU MATER": An efficient infection control for endometritis, but not for urinary tract infection after vaginal delivery.

    Science.gov (United States)

    Ayzac, Louis; Caillat-Vallet, Emmanuelle; Girard, Raphaële; Berland, Michel

    2016-09-01

    "RESEAU MATER" is useful to monitor nosocomial infections in maternity and contributes to the decreasing trend of it, since its implementation. Specifically, this network demonstrates its efficiency in the control of endometritis following vaginal deliveries, but not in the control of urinary tract infections. The aim of this study is to determine whether the difference between the control of endometritis and of urinary tract infection could be explained by an unsuitable regression model or by an unsuitable care policy concerning urinary cares. This study includes (1) the analysis of historic data of the network and (2) the description of French guidelines for maternity cares and available evaluations, concerning endometritis and urinary tract infection prevention. Univariate and multivariate odds ratios (ORs) were calculated for the total study period of 1999-2013, for these infections and their risk factors. The endometritis frequency is decreasing, in association with no significant evolution of associated risk factors, but urinary tract infection frequency is constant, in association with a increasing trend of its risk factors such as intermittent catheterization and epidural analgesia. In French guidelines, all preventive measures against endometritis are clearly broadcasted by all field operators, and repeated audits have reinforced the control of their application. But preventive measures against urinary tract infection seem to be broadcasted exclusively in the circle of infection prevention agencies and not in the obstetrics societies or in the Health Ministry communication. Urinary tract infection prevention requires a clearer public and professional policy in favor of a more efficient urinary cares, with a specific target to maternity.

  17. Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA

    Science.gov (United States)

    Schmidt, Thomas P.; Perna, Anna M.; Fugmann, Tim; Böhm, Manja; Jan Hiss; Haller, Sarah; Götz, Camilla; Tegtmeyer, Nicole; Hoy, Benjamin; Rau, Tilman T.; Neri, Dario; Backert, Steffen; Schneider, Gisbert; Wessler, Silja

    2016-03-01

    The cell adhesion protein and tumour suppressor E-cadherin exhibits important functions in the prevention of gastric cancer. As a class-I carcinogen, Helicobacter pylori (H. pylori) has developed a unique strategy to interfere with E-cadherin functions. In previous studies, we have demonstrated that H. pylori secretes the protease high temperature requirement A (HtrA) which cleaves off the E-cadherin ectodomain (NTF) on epithelial cells. This opens cell-to-cell junctions, allowing bacterial transmigration across the polarised epithelium. Here, we investigated the molecular mechanism of the HtrA-E-cadherin interaction and identified E-cadherin cleavage sites for HtrA. Mass-spectrometry-based proteomics and Edman degradation revealed three signature motifs containing the [VITA]-[VITA]-x-x-D-[DN] sequence pattern, which were preferentially cleaved by HtrA. Based on these sites, we developed a substrate-derived peptide inhibitor that selectively bound and inhibited HtrA, thereby blocking transmigration of H. pylori. The discovery of HtrA-targeted signature sites might further explain why we detected a stable 90 kDa NTF fragment during H. pylori infection, but also additional E-cadherin fragments ranging from 105 kDa to 48 kDa in in vitro cleavage experiments. In conclusion, HtrA targets E-cadherin signature sites that are accessible in in vitro reactions, but might be partially masked on epithelial cells through functional homophilic E-cadherin interactions.

  18. Allosteric Regulation of Serine Protease HtrA2 through Novel Non-Canonical Substrate Binding Pocket

    Science.gov (United States)

    Singh, Nitu; Gadewal, Nikhil; Chaganti, Lalith K.; Sastry, G. Madhavi; Bose, Kakoli

    2013-01-01

    HtrA2, a trimeric proapoptotic serine protease is involved in several diseases including cancer and neurodegenerative disorders. Its unique ability to mediate apoptosis via multiple pathways makes it an important therapeutic target. In HtrA2, C-terminal PDZ domain upon substrate binding regulates its functions through coordinated conformational changes the mechanism of which is yet to be elucidated. Although allostery has been found in some of its homologs, it has not been characterized in HtrA2 so far. Here, with an in silico and biochemical approach we have shown that allostery does regulate HtrA2 activity. Our studies identified a novel non-canonical selective binding pocket in HtrA2 which initiates signal propagation to the distal active site through a complex allosteric mechanism. This non-classical binding pocket is unique among HtrA family proteins and thus unfolds a novel mechanism of regulation of HtrA2 activity and hence apoptosis. PMID:23457469

  19. Allosteric regulation of serine protease HtrA2 through novel non-canonical substrate binding pocket.

    Directory of Open Access Journals (Sweden)

    Pruthvi Raj Bejugam

    Full Text Available HtrA2, a trimeric proapoptotic serine protease is involved in several diseases including cancer and neurodegenerative disorders. Its unique ability to mediate apoptosis via multiple pathways makes it an important therapeutic target. In HtrA2, C-terminal PDZ domain upon substrate binding regulates its functions through coordinated conformational changes the mechanism of which is yet to be elucidated. Although allostery has been found in some of its homologs, it has not been characterized in HtrA2 so far. Here, with an in silico and biochemical approach we have shown that allostery does regulate HtrA2 activity. Our studies identified a novel non-canonical selective binding pocket in HtrA2 which initiates signal propagation to the distal active site through a complex allosteric mechanism. This non-classical binding pocket is unique among HtrA family proteins and thus unfolds a novel mechanism of regulation of HtrA2 activity and hence apoptosis.

  20. 变异链球菌致龋性及酸性压力对其HtrA表达情况的影响%Influence of HtrA Expression on the Cariogenicity and the Acid Stress of Streptococcus Mutans

    Institute of Scientific and Technical Information of China (English)

    杨铁崎; 聂庆东; 郑鹏

    2013-01-01

    目的:探讨变异链球菌株HtrA的表达与其致龋性和耐酸性的关系.方法:收集了不同龋敏感性儿童口腔分离的变异链球菌共36株(高、低、无龋各12株),通过RT-PCR的方法对所有菌株HtrA的mRNA的表达水平进行了检测;此外,还检测了在分别含有10、20 mmol/L乳酸的培养基中生长的变异链球菌UA159菌株HtrAmRNA的表达变化.结果:高龋、低龋及无龋儿童口腔变异链球菌临床分离株HtrA mRNA表达存在差异,高龋组>低龋组>无龋组,差异具有统计学意义(P<0.01);酸性环境下变异链球菌中HtrA mRNA的表达水平也发生了明显上调(P<0.01).结论:HtrA基因的表达与变异链球菌的致龋性和耐酸性有着一定的相关性.%Objective: To explore the relationship between the HtrA expression of Streptococcus mutans and its cariogenicity and acid resistance.Methods: A totally of 36 isolates of Streptococcus mutans were obtained from children with different degree of dental caries (High- , low- and free - caries are 12 strains, respectively) , and the expression of HtrA mRNA of all strains were detected by RT- PCR method.Additional, the HtrA mRNA expression of S.mutans UA159 under lactic acid conditions were observed.Results: There was a difference among the HtrA mRNA expression in S.mutans isolates form Children with different degree of dental caries, High - caries group>low-caries group>free - caries group, and the difference was statistically significance (P<0.01).The HtrA mRNA expression level of S.mutans were up-regulated under acid conditions (P<0.01).Conclusion: The HtrA expression of S.mutans was related to its cariogenicity and acid resistance.

  1. Thermal Hydraulic Analysis Using GIS on Application of HTR to Thermal Recovery of Heavy Oil Reservoirs

    Directory of Open Access Journals (Sweden)

    Yangping Zhou

    2012-01-01

    Full Text Available At present, large water demand and carbon dioxide (CO2 emissions have emerged as challenges of steam injection for oil thermal recovery. This paper proposed a strategy of superheated steam injection by the high-temperature gas-cooled reactor (HTR for thermal recovery of heavy oil, which has less demand of water and emission of CO2. The paper outlines the problems of conventional steam injection and addresses the advantages of superheated steam injection by HTR from the aspects of technology, economy, and environment. A Geographic Information System (GIS embedded with a thermal hydraulic analysis function is designed and developed to analyze the strategy, which can make the analysis work more practical and credible. Thermal hydraulic analysis using this GIS is carried out by applying this strategy to a reference heavy oil field. Two kinds of injection are considered and compared: wet steam injection by conventional boilers and superheated steam injection by HTR. The heat loss, pressure drop, and possible phase transformation are calculated and analyzed when the steam flows through the pipeline and well tube and is finally injected into the oil reservoir. The result shows that the superheated steam injection from HTR is applicable and promising for thermal recovery of heavy oil reservoirs.

  2. An Estimation of Gas Pressure in a TRISO of 350 MWth Block-Type HTR

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young Min; Jo, C. K.; Cho, M. S. [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    An estimation of gas pressure in a TRISO with a UCO kernel has been performed under the normal operation conditions of a HTR. The following conclusions are drawn through the analysis. - The major gas species is xenon below 1000 .deg. C, and cesium starts to significantly build up between 1000 and 1100 .deg. C. - The lower the PF is, the earlier cesium gas begins to form and the higher the total gas pressure is. - The total gas pressure in a TRISO is about 28 MPa at temperature of 1300 .deg. C, PF of 25%, and EFPD of 1500. - The low PF is desirable on fuel economy. The analyses of stress and failure of TRISOs will be used to determine whether the pressures are tolerable or not. The HTR will be operated for an extended fuel burnup of more than 150 GWd/MTU. Its fuel should survives the long irradiation. The block-type HTR fuel is a cylindrical graphite compact in which a large number of tri-isotropic coated fuel particles (TRISOs) are embedded. A TRISO consists of a kernel at its central region and four coating layers surrounding the kernel: buffer, IPyC (inner pyrocarbon), SiC (silicon carbide), and OPyC (outer pyrocarbon), from the inside. In a usual UO{sub 2} TRISO, a very high gas pressure builds up due to the extended operation of a HTR. Nuclear fissions in a kernel produces free oxygen.

  3. Fuzzy-logic approach to HTR nuclear power plant model control

    Energy Technology Data Exchange (ETDEWEB)

    Bubak, M.; Moscinski, J. (Akademia Gorniczo-Hutnicza, Krakow (Poland)); Jewulski, J. (Institute of Physical Chemistry, Krakow (Poland))

    1983-01-01

    The fuzzy-set theory is used to incorporate linguistic 'rules of the thumb' of a human operator in the HTR nuclear power plant controller. The results of the extensive computer simulations are encouraging and confirm the usefulness of this approach in nuclear power plant control. In the Appendix, a short introduction to fuzzy logic is given.

  4. Oxidation Analyses of Massive Air Ingress Accident of HTR-PM

    Directory of Open Access Journals (Sweden)

    Wei Xu

    2016-01-01

    Full Text Available The double-ended guillotine break (DEGB of the horizontal coaxial gas duct accident is a serious air ingress accident of the high temperature gas-cooled reactor pebble-bed module (HTR-PM. Because the graphite is widely used as the structure material and the fuel element matrix of HTR-PM, the oxidation analyses of this severe air ingress accident have got enough attention in the safety analyses of the HTR-PM. The DEGB of the horizontal coaxial gas duct accident is calculated by using the TINTE code in this paper. The results show that the maximum local oxidation of the matrix graphite of spherical fuel elements in the core will firstly reach 3.75⁎104 mol/m3 at about 120 h, which means that only the outer 5 mm fuel-free zone of matrix graphite will be oxidized out. Even at 150 h, the maximum local weight loss ratio of the nuclear grade graphite in the bottom reflectors is only 0.26. Besides, there is enough time to carry out some countermeasures to stop the air ingress during several days. Therefore, the nuclear grade graphite of the bottom reflectors will not be fractured in the DEGB of the horizontal coaxial gas duct accident and the integrity of the HTR-PM can be guaranteed.

  5. True but not false memories are associated with the HTR2A gene.

    Science.gov (United States)

    Zhu, Bi; Chen, Chuansheng; Loftus, Elizabeth F; Moyzis, Robert K; Dong, Qi; Lin, Chongde

    2013-11-01

    Previous research reported that serotonin receptor 2A gene (HTR2A) polymorphisms were associated with memory. However, it is unknown whether these genetic variants were associated with both true and false memories. The current study of 336 Han Chinese subjects tested 30 single nucleotide polymorphisms (SNPs) within the HTR2A gene for potential associations with true and false memories. False memories were assessed using the Deese-Roediger-McDermott (DRM) paradigm, in which people falsely remember semantically related (but unpresented) words. We found that 11 SNPs within the HTR2A gene were associated with true memory (p=0.000076-0.043). The associations between true memory and seven adjacent SNPs (i.e., rs1923888, rs1745837, rs9567739, rs3742279, rs655888, rs655854, and rs2296972) were still significant after multiple testing corrections. Haplotype-based association analysis revealed that, true memory was positively associated with haplotype A-C-C-G-C-T-A for these seven adjacent SNPs (p=0.000075), which was still significant after multiple testing correction. Only one SNP rs655854 was associated with false memory (p=0.023), and it was not significant after multiple testing correction. This study replicates, in an Asian population, that genetic variation in HTR2A is associated with episodic memory, and also suggests that this association is restricted to true memory.

  6. In-situ hybridization based quantification of hTR: a possible biomarker in malignant melanoma

    DEFF Research Database (Denmark)

    Vagner, Josephine; Steiniche, Torben; Stougaard, Magnus

    2015-01-01

    Aims Telomerase is reactivated in most cancers and there is accumulating evidence for it being a driver event in malignant melanoma (MM). Thus, our aim was to evaluate if in situ hybridisation (ISH)-based quantification of the telomerase RNA (hTR) could be used to distinguish MM from nevi and if ...

  7. htrA、clpP基因缺失对变异链球菌生长影响的初步观察%Effect of htrA and clpP Mutation on the Growth of Streptococcus Mutans

    Institute of Scientific and Technical Information of China (English)

    季晓黎; 于丹妮; 王拓; 李娜; 韩育植

    2012-01-01

    Objective: To study the effect of high temperature requirement A (htrA) and caseinolytic protease P (clp P) on the growth of streptococcus mutans. Methods: The nephelometer method was used to measure the absorbance of the culture medium each hour in six hours including the streptococcus mutans standard strain, htrA, clpP, and htrA-clpP mutant strains. MTT method was used to detect the absorbance of formazan at different time points, and calculate the linear relation between those and quantity of living bacterium measured by viable plate count. Results: In the normal nutrition condition, the growth of streptococcus mutans standard strain was significantly higher than that of the mutant strains of htrA and htrA-clpP detected by nephelometer method in 1-6 h and MTT method in 2 h and 4 h (P < 0.01). The growth of clpP mutant strain was significantly higher than that of other two mutant strains using nephelometer method in 1-6 h and MTT method in 1-3 h (P < 0.01). For streptococcus mutans standard strain, htrA and htrA-clpP mutant strains in 1-4 h and clpP mutant strain inl-3 h, absorbance of formazan was positively related to the concentration of the bacteria (r = 0.860, 0.761,0.790 and 0.773, P < 0.05 or P < 0.01). Conclusion: In this study, streptococcus mutans were inhibited by htrA or clpP deletion simul-taneouly and respectively. MTT method was used to gain the quantity of living bacterium of four strains in the range of the specific bacterial count and in a given duration.%目的:研究htrA、clpP基因缺失对变异链球菌生长情况的影响.方法:采用浊度法测定变异链球菌标准株、htrA单基因缺陷株、clpP单基因缺陷株及htrA-clpP双基因缺陷株生长6h中每小时的吸光度,采用MTT实验测定4种菌株的不同时间甲臜吸光度变化情况并探究其与平板计数活菌量之间的关系.结果:在正常营养条件下,浊度法1~6h及MTT法2、4h及6h变异链球菌标准株的生长快于htrA、htr

  8. Distinct 3D Architecture and Dynamics of the Human HtrA2(Omi) Protease and Its Mutated Variants

    Science.gov (United States)

    Gieldon, Artur; Zurawa-Janicka, Dorota; Jarzab, Miroslaw; Wenta, Tomasz; Golik, Przemyslaw; Dubin, Grzegorz; Lipinska, Barbara; Ciarkowski, Jerzy

    2016-01-01

    HtrA2(Omi) protease controls protein quality in mitochondria and plays a major role in apoptosis. Its HtrA2S306A mutant (with the catalytic serine routinely disabled for an X-ray study to avoid self-degradation) is a homotrimer whose subunits contain the serine protease domain (PD) and the regulatory PDZ domain. In the inactive state, a tight interdomain interface limits penetration of both PDZ-activating ligands and PD substrates into their respective target sites. We successfully crystalized HtrA2V226K/S306A, whose active counterpart HtrA2V226K has had higher proteolytic activity, suggesting higher propensity to opening the PD-PDZ interface than that of the wild type HtrA2. Yet, the crystal structure revealed the HtrA2V226K/S306A architecture typical of the inactive protein. To get a consistent interpretation of crystallographic data in the light of kinetic results, we employed molecular dynamics (MD). V325D inactivating mutant was used as a reference. Our simulations demonstrated that upon binding of a specific peptide ligand NH2-GWTMFWV-COOH, the PDZ domains open more dynamically in the wild type protease compared to the V226K mutant, whereas the movement is not observed in the V325D mutant. The movement relies on a PDZ vs. PD rotation which opens the PD-PDZ interface in a lid-like (budding flower-like in trimer) fashion. The noncovalent hinges A and B are provided by two clusters of interfacing residues, harboring V325D and V226K in the C- and N-terminal PD barrels, respectively. The opening of the subunit interfaces progresses in a sequential manner during the 50 ns MD simulation. In the systems without the ligand only minor PDZ shifts relative to PD are observed, but the interface does not open. Further activation-associated events, e.g. PDZ-L3 positional swap seen in any active HtrA protein (vs. HtrA2), were not observed. In summary, this study provides hints on the mechanism of activation of wtHtrA2, the dynamics of the inactive HtrA2V325D, but does not

  9. Associations of the 5-hydroxytryptamine (Serotonin) Receptor 1B Gene (HTR1B) with Alcohol, Cocaine, and Heroin Abuse

    OpenAIRE

    Cao, Jian; LaRocque, Emily; Li, Dawei

    2013-01-01

    Abnormal serotonergic pathways are implicated in numerous neuropsychiatric disorders including alcohol and drug dependence (abuse). The human 5-hydroxytryptamine (serotonin) receptor 1B, encoded by the HTR1B (5-HT1B) gene, is a presynaptic serotonin autoreceptor that plays an important role in regulating serotonin synthesis and release. Although there was evidence of associations of the HTR1B gene variants in the etiologies of substance use disorders, negative findings were also reported. To ...

  10. Human telomerase reverse transcriptase binds to a pre-organized hTR in vivo exposing its template.

    Science.gov (United States)

    Zemora, Georgeta; Handl, Stefan; Waldsich, Christina

    2016-01-08

    Telomerase is a specialized reverse transcriptase that is responsible for telomere length maintenance. As in other organisms, the minimal components required for an active human telomerase are the template-providing telomerase RNA (hTR) and the enzymatic entity telomerase reverse transcriptase (hTERT). Here, we explored the structure of hTR and the hTERT-induced conformational changes within hTR in living cells. By employing an in vivo DMS chemical probing technique, we showed that the pseudoknot and associated triple helical scaffold form stably in vivo independently of hTERT. In fact, the dimethyl-sulfate (DMS) modification pattern suggests that hTR alone is capable of adopting a conformation that is suited to interact with hTERT. However, in the absence of hTERT the template region of hTR is only weakly accessible to DMS-modifications. The predominant change after binding of hTERT to hTR is the exposure of the template region.

  11. Methylphenidate to adolescent rats drives enduring changes of accumbal Htr7 expression: implications for impulsive behavior and neuronal morphology.

    Science.gov (United States)

    Leo, D; Adriani, W; Cavaliere, C; Cirillo, G; Marco, E M; Romano, E; di Porzio, U; Papa, M; Perrone-Capano, C; Laviola, G

    2009-04-01

    Methylphenidate (MPH) administration to adolescent rodents produces persistent region-specific changes in brain reward circuits and alterations of reward-based behavior. We show that these modifications include a marked increment of serotonin (5-hydroxy-tryptamine) receptor type 7 (Htr7) expression and synaptic contacts, mainly in the nucleus accumbens, and a reduction of basal behavioral impulsivity. We show that neural and behavioral consequences are functionally related: administration of a selective Htr7 antagonist fully counteracts the MPH-reduced impulsive behavior and enhances impulsivity when administered alone in naive rats. Agonist-induced activation of endogenous Htr7 significantly increases neurite length in striatal neuron primary cultures, thus suggesting plastic remodeling of neuronal morphology. The mixed Htr (1a/7) agonist, 8-OH-DPAT, reduces impulsive behavior in adolescent rats and in naive adults, whose impulsivity is enhanced by the Htr7 antagonist. In summary, behavioral pharmacology experiments show that Htr7 mediates self-control behavior, and brain primary cultures experiments indicate that this receptor may be involved in the underlying neural plasticity, through changes in neuronal cytoarchitecture.

  12. Recombinant Deg/HtrA proteases from Synechocystis sp. PCC 6803 differ in substrate specificity, biochemical characteristics and mechanism

    Science.gov (United States)

    Huesgen, Pitter F.; Miranda, Helder; Lam, XuanTam; Perthold, Manuela; Schuhmann, Holger; Adamska, Iwona; Funk, Christiane

    2011-01-01

    Cyanobacteria require efficient protein-quality-control mechanisms to survive under dynamic, often stressful, environmental conditions. It was reported that three serine proteases, HtrA (high temperature requirement A), HhoA (HtrA homologue A) and HhoB (HtrA homologue B), are important for survival of Synechocystis sp. PCC 6803 under high light and temperature stresses and might have redundant physiological functions. In the present paper, we show that all three proteases can degrade unfolded model substrates, but differ with respect to cleavage sites, temperature and pH optima. For recombinant HhoA, and to a lesser extent for HtrA, we observed an interesting shift in the pH optimum from slightly acidic to alkaline in the presence of Mg2+ and Ca2+ ions. All three proteases formed different homo-oligomeric complexes with and without substrate, implying mechanistic differences in comparison with each other and with the well-studied Escherichia coli orthologues DegP (degradation of periplasmic proteins P) and DegS. Deletion of the PDZ domain decreased, but did not abolish, the proteolytic activity of all three proteases, and prevented substrate-induced formation of complexes higher than trimers by HtrA and HhoA. In summary, biochemical characterization of HtrA, HhoA and HhoB lays the foundation for a better understanding of their overlapping, but not completely redundant, stress-resistance functions in Synechocystis sp. PCC 6803. PMID:21332448

  13. Raphael: materials and components highlights from the HTR FP5 and FP6 programmes

    Energy Technology Data Exchange (ETDEWEB)

    Buckthorpe, D. [AMEC NNC Limited, Knutsford (United Kingdom); Breuil, E.; Besson, D.; Gelineau, O.; Falcand, C. [AREVA-NP SAS, 69 - Lyon (France); Davies, M. [AMECNNC Ltd., Knutsford (United Kingdom); Lejeail, Y. [CEA Cadarache, 13 - Saint Paul lez Durance (France); Hugon, I. [CEA Valrho, 30 - Marcoule (France); Briottet, L.; Tochon, P. [CEA Grenoble, 38 (France); Hegeman, J.; Vreeling, A. [Nuclear Research and Consultancy Group (NRG), Petten (Netherlands); Hurst, R. [European Commission, Joint Research Centre (JRC), Institute for Advanced Materials, Petten, (Netherlands); Von Lensa, W.; Schogl, B. [Forschungszentrum Juelich GmbH (FZJ..ISR) (Germany); Der Lorenzo, D. [Empresarios Agrupados Internacional S A (EASA.MD) (Spain); Friedrich, B.C. [AREVA-NP GmbH, Erlangen (Germany); Blatt, M. [Electricitie de France (EdF) (France); Marek, M. [NRI Rez (Czech Republic); Chen, J. [Paul Sherer Institute (PSI) (Switzerland); Hingst, K.; Tahon, B. [SGL Carbon (France); Homerin, P. [Graphtech Intern Ltd (France); Hall, G. [Manchester Univ. (United Kingdom); Cucini, D. [Jeumont (France); Gronek, M. [Zittau Univ. (Germany)

    2007-07-01

    Good progress has been made on the Very High Temperature Reactor (VHTR) material and components investigations performed within the European Commission fifth and sixth Framework programmes. These programmes focus on the identification and investigation of key technological issues for this modular type of reactor, which offers significant advantages for the long-term development of sustainable energy and heat applications including hydrogen generation. For the fifth Framework programme two materials projects HTR-M and M1 and a component project (HTR-E) were undertaken to address requirements for the reactor pressure boundary, high temperature components (including turbine and recuperator), tribology issues and operational issues and the graphite core. For the sixth Framework programme the RAPHAEL-IP is underway addressing the remaining key issues plus extending the development and qualification to include heat exchangers, the gas circulator and design codes. The main highlights of the results from the key investigations and tests performed within these programmes are summarized. (authors)

  14. Feasibility of Burning Civilian Grade Pu in the Modular HTR with Th Fuel Cycle

    Institute of Scientific and Technical Information of China (English)

    许云林; 经荥清

    2001-01-01

    The Modular High Temperature Gas-Cooled Reactor (HTR) can be usedto burn plutonium fuel to reduce Pu stockpiles because of its inherent safety characteristics and ability to burn a variety of fuel mixtures. The equilibrium core is calculated and analyzed for Pu enriched fuel. Fuel spheres with 7 g heavy metal including the civilian grade Pu and thorium are loaded into the reactor. An enrichment of 11% is chosen to provide the desired equilibrium core reactivity. The fuel and moderator temperature coefficients are both negative. The maximum fuel element temperature during normal operation and during a loss of coolant accident is less than 1500 ℃. 92% of 239Pu will be burnt during nomal operation. Therefore, a thorium fuel cycle in the modular HTR is an effective method for burning civilian grade plutonium.

  15. K {sub infinity} measurements of HTR lattices by oscillated zero reactivity technique

    Energy Technology Data Exchange (ETDEWEB)

    Benedetti, F.; Brighenti, G.; Chiodi, P.L.; Ghilardotti, G.; Guiliani, C.

    1972-06-15

    The principles of the K {sub infinity} measurement of a HTR lattice, carried out by the oscillated zero reactivity method have been reported at the 9th DCPM. The RB-2 reactor modifications, requested for these measurements have been shown at the 10th DCPM. This report summarizes the modified RB-2 reactor characteristics, the present status of measurements, some preliminary results, and, the next measurements program. The report reflects the status of the work at the end of May 1972.

  16. STUDI PEMODELAN DAN PERHITUNGAN TRANSPORT MONTE CARLO DALAM TERAS HTR PEBBLE BED

    Directory of Open Access Journals (Sweden)

    Zuhair .

    2013-01-01

    Full Text Available Konsep sistem energi VHTR baik yang berbahan bakar pebble (VHTR pebble bed maupun blok prismatik (VHTR prismatik menarik perhatian fisikawan reaktor nuklir. Salah satu kelebihan teknologi bahan bakar bola adalah menawarkan terobosan teknologi pengisian bahan bakar tanpa harus menghentikan produksi listrik. Selain itu, partikel bahan bakar pebble dengan kernel uranium oksida (UO2 atau uranium oksikarbida (UCO yang dibalut TRISO dan pelapisan silikon karbida (SiC dianggap sebagai opsi utama dengan pertimbangan performa tinggi pada burn-up bahan bakar dan temperatur tinggi. Makalah ini mendiskusikan pemodelan dan perhitungan transport Monte Carlo dalam teras HTR pebble bed. HTR pebble bed adalah reaktor berpendingin gas temperatur tinggi dan bermoderator grafit dengan kemampuan kogenerasi. Perhitungan dikerjakan dengan program MCNP5 pada temperatur 1200 K. Pustaka data nuklir energi kontinu ENDF/B-V dan ENDF/B-VI dimanfaatkan untuk melengkapi analisis. Hasil perhitungan secara keseluruhan menunjukkan konsistensi dengan nilai keff yang hampir sama untuk pustaka data nuklir yang digunakan. Pustaka ENDF/B-VI (66c selalu memproduksi keff lebih besar dibandingkan ENDF/B-V (50c maupun ENDF/B-VI (60c dengan bias kurang dari 0,25%. Kisi BCC memprediksi keff hampir selalu lebih kecil daripada kisi lainnya, khususnya FCC. Nilai keff kisi BCC lebih dekat dengan kisi FCC dengan bias kurang dari 0,19% sedangkan dengan kisi SH bias perhitungannya kurang dari 0,22%. Fraksi packing yang sedikit berbeda (BCC= 61%, SH= 60,459% tidak membuat bias perhitungan menjadi berbeda jauh. Estimasi keff ketiga model kisi menyimpulkan bahwa model BCC lebih bisa diadopsi dalam perhitungan HTR pebble bed dibandingkan model FCC dan SH. Verifikasi hasil estimasi ini perlu dilakukan dengan simulasi Monte Carlo atau bahkan program deterministik lainnya guna optimisasi perhitungan teras reaktor temperatur tinggi.   Kata-kunci: kernel, TRISO, bahan bakar pebble, HTR pebble bed

  17. Preliminary Reactor Physics Assessment of the HTR Module with 14% Enriched UCO Fuel

    Energy Technology Data Exchange (ETDEWEB)

    Gerhard Strydom; Hans D. Gougar

    2013-03-01

    The high temperature reactor (HTR) Module (Lohnert, 1990) is a graphite-moderated, helium cooled pebble bed design that has been extensively used as a reference template for the former South African (Matzner, 2004) and current Chinese (Zhang et al., 2009) HTR programs. This design utilizes spherical fuel elements packed into a dynamic pebble bed, consisting of tri-structural isotropic (TRISO) coated uranium oxide (UO2) 500 µm fuel kernels with a U-235 enrichment of 7.8% and a heavy metal loading of 7 g per pebble. This fuel type was previously qualified for use in Germany for pebble bed HTRs, as well as undergoing re-qualification in South Africa for the PBMR project. It is also the fuel type being tested for use in the high temperature reactor (HTR-PM) under construction in China. In the United States, however, a different TRISO fuel form is the subject of a qualification program. The U.S. experience with HTRs has been focused upon the batch-fueled prismatic reactor in which TRISO particles are embedded in cylindrical compacts and stacked inside the graphite blocks which comprise the core. Under this type of operating regime, a smaller TRISO with a different composition and enrichment performs better than the fuel historically used in PBRs. Fuel kernels and compacting techniques more suited to prismatic core duty are currently being developed and qualified under the U.S. Department of Energy's Advanced Gas Reactor (AGR) fuel development program and in support of the Next Generation Nuclear Plant project. Interest in the pebble bed concept remains high, however, and a study was undertaken by the authors to assess the viability of using AGR fuel in a pebble bed reactor. Using the German HTR Module as the reference plant, key neutronic and thermal-hydraulic parameters were compared between the nominal design and one fueled with the fuel that is the focus of the AGR program.

  18. Temperature Response of the HTR-10 during the Power Ascension Test

    Directory of Open Access Journals (Sweden)

    Fubing Chen

    2015-01-01

    Full Text Available The 10 MW High Temperature Gas-Cooled Reactor-Test Module (HTR-10 is the first High Temperature Gas-Cooled Reactor in China. With the objective of raising the reactor power from 30% to 100% rated power, the power ascension test was planned and performed in January 2003. The test results verified the practicability and validity of the HTR-10 power regulation methods. In this study, the power ascension process is preliminarily simulated using the THERMIX code. The code satisfactorily reproduces the reactor transient parameters, including the reactor power, the primary helium pressure, and the primary helium outlet temperature. Reactor internals temperatures are also calculated and compared with the test values recorded by a number of thermocouples. THERMIX correctly simulates the temperature variation tendency for different measuring points, with good to fair agreement between the calculated temperatures and the measured ones. Based on the comparison results, the THERMIX simulation capability for the HTR-10 dynamic characteristics during the power ascension process can be demonstrated. With respect to the reactor safety features, it is of utmost importance that the maximum fuel center temperature during the test process is always much lower than the fuel temperature limit of 1620°C.

  19. Etude simulée d'un système de dessalement d'eau de mer et de production de froid par thermofrigopompe couplée à des panneaux solaires

    OpenAIRE

    Byrne, Paul; Miriel, Jacques; Serres, Laurent; Ghoubali, Redouane

    2012-01-01

    International audience; L'accès à l'eau potable est un des enjeux majeurs des prochaines decennies. La recherche sur les technologies de dessalement à forte efficacité énergétique doit donc être particulièrement active. Cet article présente un système de thermofrigopompe produisant simultanément du froid pour une enceinte réfrigérée et de la chaleur utilisée par une unité de dessalement par distillation membranaire. L'énergie électrique est fournie par des panneaux photovoltaïques pour une po...

  20. Proteomic approaches to identify substrates of the three Deg/HtrA proteases of the cyanobacterium Synechocystis sp. PCC 6803.

    Science.gov (United States)

    Tam, Lam X; Aigner, Harald; Timmerman, Evy; Gevaert, Kris; Funk, Christiane

    2015-06-15

    The family of Deg/HtrA proteases plays an important role in quality control of cellular proteins in a wide range of organisms. In the genome of the cyanobacterium Synechocystis sp. PCC 6803, a model organism for photosynthetic research and renewable energy products, three Deg proteases are encoded, termed HhoA, HhoB and HtrA. In the present study, we compared wild-type (WT) Synechocystis cells with the single insertion mutants ΔhhoA, ΔhhoB and ΔhtrA. Protein expression of the remaining Deg/HtrA proteases was strongly affected in the single insertion mutants. Detailed proteomic studies using DIGE (difference gel electrophoresis) and N-terminal COFRADIC (N-terminal combined fractional diagonal chromatography) revealed that inactivation of a single Deg protease has similar impact on the proteomes of the three mutants; differences to WT were observed in enzymes involved in the major metabolic pathways. Changes in the amount of phosphate permease system Pst-1 were observed only in the insertion mutant ΔhhoB. N-terminal COFRADIC analyses on cell lysates of ΔhhoB confirmed changed amounts of many cell envelope proteins, including the phosphate permease systems, compared with WT. In vitro COFRADIC studies were performed to identify the specificity profiles of the recombinant proteases rHhoA, rHhoB or rHtrA added to the Synechocystis WT proteome. The combined in vivo and in vitro N-terminal COFRADIC datasets propose RbcS as a natural substrate for HhoA, PsbO for HhoB and HtrA and Pbp8 for HtrA. We therefore suggest that each Synechocystis Deg protease protects the cell through different, but connected mechanisms. © The Authors Journal compilation © 2015 Biochemical Society.

  1. Polymorphisms in HTR2A and DRD4 Predispose to Smoking and Smoking Quantity

    Science.gov (United States)

    Pérez-Rubio, Gloria; Ramírez-Venegas, Alejandra; Noé Díaz, Valeri; García Gómez, Leonor; Elvira Fabián, Karina; García Carmona, Salvador; López-Flores, Luis A.; Ambrocio-Ortiz, Enrique; Contreras Romero, Rocío; Alcantar-Ayala, Noé; Sansores, Raúl H.

    2017-01-01

    Background Genes encoding the receptors involved in the dopaminergic and serotonergic pathways are potential candidates in the mechanisms of nicotine addiction. Aims To identify genetic variants in the promoter regions and exons of the DRD4 and HTR2A genes associated with tobacco smoking and the degree of nicotine addiction in Mexican mestizos. Methods The study included 438 non-smokers (NS) and 1,157 current smokers, ranked based on their consumption of cigarettes per day (cpd): 574 heavy smokers (HS, >20 cpd) and 583 light smokers (LS, 1–10 cpd). Genotyping was performed for 4 and 8 single nucleotide polymorphisms (SNPs) in the DRD4 and HTR2A genes, respectively. Results The C allele of rs1800955 in DRD4 was found to be associated with cigarette smoking in the HS vs. NS and LS vs. NS comparisons (p = 2.34E-03 and p = 1.13E-03, respectively); the association was maintained in the homozygous CC genotype (p = 5.00E-04 and p = 2.00E-04, respectively). The T allele of rs6313 in HTR2A was significantly associated with cigarette smoking and a greater degree of nicotine addiction (p = 4.77E-03, OR = 1.55); the association was maintained in the homozygous genotype (TT) (p = 4.90E-03, OR = 1.96). The A allele of rs6313 was associated with cigarette smoking in the HS vs. NS comparison (p = 1.53E-02, OR = 1.36); the risk was nearly doubled in the homozygous AA genotype (p = 1.30E-03, OR = 1.83) compared with the heterozygous GA genotype (OR = 1.38). Conclusions Among Mexican mestizos, the C allele of rs1800955 in the DRD4 gene and the A allele of rs6311 in the HTR2A gene are associated with cigarette smoking, whereas the T allele of rs6313 in HTR2A is associated with cigarette smoking and the degree of nicotine addiction. PMID:28103253

  2. Thermal-hydraulic design and transient evaluation of a small long-life HTR

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Ming [Harbin Engineering University, Nantong Street 145, 150001 Harbin (China); Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands); Kloosterman, Jan Leen, E-mail: j.l.kloosterman@tudelft.nl [Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands)

    2013-02-15

    Highlights: ► We present the thermal-hydraulic evaluations of a small, long-life and block-type HTR using the DALTON/THERMIX code system. ► A cross section generation methodology is developed and verified for the diffusion calculations of the small HTR. ► The thermal-hydraulic characteristics of the small HTR during pressurized loss of forced-cooling incidents are compared with depressurized loss of forced-cooling ones. ► The thermal-hydraulic characteristics of a cylindrical core are compared with an annular one. ► Thermal power limit of the small HTR is investigated based on depressurized loss of forced-cooling incidents. -- Abstract: Small long-life high temperature gas-cooled reactors (HTRs) may provide electricity or heat for remote areas or industrial users in developed and/or developing countries. Moreover, small HTRs have advantages over large nuclear reactors of demonstrated inherent safety, transportability, modular construction, and flexible site selection. This paper presents the thermal-hydraulic evaluations of the U-Battery, which is a small, long-life and block-type HTR using the DALTON/THERMIX code system. The thermal-hydraulic characteristics of a cylindrical design and an annular design of the U-Battery were evaluated for loss of forced-cooling (LOFC) incidents including depressurized LOFC (DLOFC) and pressurized LOFC (PLOFC) incidents. The calculations show that the stronger natural circulation during the PLOFC makes the reactor core cool faster than during the DLOFC, flattens the radial solid temperature distribution, and transfers more heat from the hot regions (bottom and center of the reactor core) to cold regions (top and periphery of the reactor core). Although the natural circulation in the reactor core is so weak that it is neglected during the DLOFC, the decay heat is removed passively by conduction without any violation of the temperature limits for the 20 MWth U-Battery. The comparisons of the cylindrical and annular reactor

  3. High Temperature Reactor (HTR) Deep Burn Core and Fuel Analysis: Design Selection for the Prismatic Block Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Francesco Venneri; Chang-Keun Jo; Jae-Man Noh; Yonghee Kim; Claudio Filippone; Jonghwa Chang; Chris Hamilton; Young-Min Kim; Ji-Su Jun; Moon-Sung Cho; Hong-Sik Lim; MIchael A. Pope; Abderrafi M. Ougouag; Vincent Descotes; Brian Boer

    2010-09-01

    The Deep Burn (DB) Project is a U.S. Department of Energy sponsored feasibility study of Transuranic Management using high burnup fuel in the high temperature helium cooled reactor (HTR). The DB Project consists of seven tasks: project management, core and fuel analysis, spent fuel management, fuel cycle integration, TRU fuel modeling, TRU fuel qualification, and HTR fuel recycle. In the Phase II of the Project, we conducted nuclear analysis of TRU destruction/utilization in the HTR prismatic block design (Task 2.1), deep burn fuel/TRISO microanalysis (Task 2.3), and synergy with fast reactors (Task 4.2). The Task 2.1 covers the core physics design, thermo-hydraulic CFD analysis, and the thermofluid and safety analysis (low pressure conduction cooling, LPCC) of the HTR prismatic block design. The Task 2.3 covers the analysis of the structural behavior of TRISO fuel containing TRU at very high burnup level, i.e. exceeding 50% of FIMA. The Task 4.2 includes the self-cleaning HTR based on recycle of HTR-generated TRU in the same HTR. Chapter IV contains the design and analysis results of the 600MWth DB-HTR core physics with the cycle length, the average discharged burnup, heavy metal and plutonium consumptions, radial and axial power distributions, temperature reactivity coefficients. Also, it contains the analysis results of the 450MWth DB-HTR core physics and the analysis of the decay heat of a TRU loaded DB-HTR core. The evaluation of the hot spot fuel temperature of the fuel block in the DB-HTR (Deep-Burn High Temperature Reactor) core under full operating power conditions are described in Chapter V. The investigated designs are the 600MWth and 460MWth DB-HTRs. In Chapter VI, the thermo-fluid and safety of the 600MWth DB-HTRs has been analyzed to investigate a thermal-fluid design performance at the steady state and a passive safety performance during an LPCC event. Chapter VII describes the analysis results of the TRISO fuel microanalysis of the 600MWth and 450

  4. The effects of applying silicon carbide coating on core reactivity of pebble-bed HTR in water ingress accident

    Energy Technology Data Exchange (ETDEWEB)

    Zuhair, S.; Setiadipura, Topan [National Nuclear Energy Agency of Indonesia, Serpong Tagerang Selatan (Indonesia). Center for Nuclear Reactor Technology and Safety; Su' ud, Zaki [Bandung Institute of Technology (Indonesia). Dept. of Physics

    2017-03-15

    Graphite is used as the moderator, fuel barrier material, and core structure in High Temperature Reactors (HTRs). However, despite its good thermal and mechanical properties below the radiation and high temperatures, it cannot avoid corrosion as a consequence of an accident of water/air ingress. Degradation of graphite as a main HTR material and the formation of dangerous CO gas is a serious problem in HTR safety. One of the several steps that can be adopted to avoid or prevent the corrosion of graphite by the water/air ingress is the application of a thin layer of silicon carbide (SiC) on the surface of the fuel element. This study investigates the effect of applying SiC coating on the fuel surfaces of pebble-bed HTR in water ingress accident from the reactivity points of view. A series of reactivity calculations were done with the Monte Carlo transport code MCNPX and continuous energy nuclear data library ENDF/B-VII at temperature of 1200 K. Three options of UO{sub 2}, PuO{sub 2}, and ThO{sub 2}/UO{sub 2} fuel kernel were considered to obtain the inter comparison of the core reactivity of pebble-bed HTR in conditions of water/air ingress accident. The calculation results indicated that the UO{sub 2}-fueled pebble-bed HTR reactivity was slightly reduced and relatively more decreased when the thickness of the SiC coating increased. The reactivity characteristic of ThO{sub 2}/UO{sub 2}-fueled pebble-bed HTR showed a similar trend to that of UO{sub 2}, but did not show reactivity peak caused by water ingress. In contrast with UO{sub 2}- and ThO{sub 2}-fueled pebble-bed HTR, although the reactivity of PuO{sub 2}-fueled pebble-bed HTR was the lowest, its characteristics showed a very high reactivity peak (0.33 Δk/k) and this introduction of positive reactivity is difficult to control. SiC coating on the surface of the plutonium fuel pebble has no significant impact. From the comparison between reactivity characteristics of uranium, thorium and plutonium cores with 0

  5. Gene structure and expression of serotonin receptor HTR2C in hypothalamic samples from infanticidal and control sows

    Directory of Open Access Journals (Sweden)

    Quilter Claire R

    2012-04-01

    Full Text Available Abstract Background The serotonin pathways have been implicated in behavioural phenotypes in a number of species, including human, rat, mouse, dog and chicken. Components of the pathways, including the receptors, are major targets for drugs used to treat a variety of physiological and psychiatric conditions in humans. In our previous studies we have identified genetic loci potentially contributing to maternal infanticide in pigs, which includes a locus on the porcine X chromosome long arm. The serotonin receptor HTR2C maps to this region, and is therefore an attractive candidate for further study based on its function and its position in the genome. Results In this paper we describe the structure of the major transcripts produced from the porcine HTR2C locus using cDNA prepared from porcine hypothalamic and pooled total brain samples. We have confirmed conservation of sites altered by RNA editing in other mammalian species, and identified polymorphisms in the gene sequence. Finally, we have analysed expression and editing of HTR2C in hypothalamus samples from infanticidal and control animals. Conclusions The results confirm that although the expression of the long transcriptional variant of HTR2C is raised in infanticidal animals, the overall patterns of editing in the hypothalamus are similar between the two states. Sequences associated with the cDNA and genomic structures of HTR2C reported in this paper are deposited in GenBank under accession numbers FR720593, FR720594 and FR744452.

  6. Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae.

    Science.gov (United States)

    Ribeiro-Guimarães, Michelle Lopes; Marengo, Eliana Blini; Tempone, Antonio Jorge; Amaral, Julio Jablonski; Klitzke, Clécio F; Silveira, Erika K Xavier da; Portaro, Fernanda Calheta Vieira; Pessolani, Maria Cristina Vidal

    2009-12-01

    Members of the high temperature requirement A (HtrA) family of chaperone proteases have been shown to play a role in bacterial pathogenesis. In a recent report, we demonstrated that the gene ML0176, which codes for a predicted HtrA-like protease, a gene conserved in other species of mycobacteria, is transcribed by Mycobacterium leprae in human leprosy lesions. In the present study, the recombinant ML0176 protein was produced and its enzymatic properties investigated. M. lepraerecombinant ML0176 was able to hydrolyse a variety of synthetic and natural peptides. Similar to other HtrA proteins, this enzyme displayed maximum proteolytic activity at temperatures above 40 degrees C and was completely inactivated by aprotinin, a protease inhibitor with high selectivity for serine proteases. Finally, analysis of M. leprae ML0176 specificity suggested a broader cleavage preference than that of previously described HtrAs homologues. In summary, we have identified an HtrA-like protease in M. lepraethat may constitute a potential new target for the development of novel prophylactic and/or therapeutic strategies against mycobacterial infections.

  7. Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae

    Directory of Open Access Journals (Sweden)

    Michelle Lopes Ribeiro-Guimarães

    2009-12-01

    Full Text Available Members of the high temperature requirement A (HtrA family of chaperone proteases have been shown to play a role in bacterial pathogenesis. In a recent report, we demonstrated that the gene ML0176, which codes for a predicted HtrA-like protease, a gene conserved in other species of mycobacteria, is transcribed by Mycobacterium leprae in human leprosy lesions. In the present study, the recombinant ML0176 protein was produced and its enzymatic properties investigated. M. lepraerecombinant ML0176 was able to hydrolyse a variety of synthetic and natural peptides. Similar to other HtrA proteins, this enzyme displayed maximum proteolytic activity at temperatures above 40°C and was completely inactivated by aprotinin, a protease inhibitor with high selectivity for serine proteases. Finally, analysis of M. leprae ML0176 specificity suggested a broader cleavage preference than that of previously described HtrAs homologues. In summary, we have identified an HtrA-like protease in M. lepraethat may constitute a potential new target for the development of novel prophylactic and/or therapeutic strategies against mycobacterial infections.

  8. batiment SHM|SHM building|Control valves on top of a ´cold box´ for the 4K refrigeration system for the LHC |Valves de contrôle sur le dessus de la ´boite froide´ pour le système de refroidissement 4K du LHC

    CERN Multimedia

    Valeriane Duvivier

    2001-01-01

    batiment SHM|SHM building|Control valves on top of a ´cold box´ for the 4K refrigeration system for the LHC |Valves de contrôle sur le dessus de la ´boite froide´ pour le système de refroidissement 4K du LHC

  9. batiment SHM|SHM building|Control valves on top of a ´cold box´ for the 4K refrigeration system for the LHC |Valves de contrôle sur le dessus de la ´boite froide´ pour le système de refroidissement 4K du LHC

    CERN Multimedia

    Valeriane Duvivier

    2001-01-01

    batiment SHM|SHM building|Control valves on top of a ´cold box´ for the 4K refrigeration system for the LHC |Valves de contrôle sur le dessus de la ´boite froide´ pour le système de refroidissement 4K du LHC

  10. Effect of leptin on HTR8-SVneo cell proliferation in vitro and its mechanism%瘦素对HTR8-SVneo细胞增殖的影响及调控机制

    Institute of Scientific and Technical Information of China (English)

    程欢欢; 王华阳; 董召刚; 徐小飞; 孔北华; 曲迅

    2012-01-01

    Objective To investigate the effects of leptin on proliferation of HTR8-Svneo cells in vitro and the level of ID (inhibitor of DNA binding and differentiation), and to explore the regulatory mechanism of leptin underlying HTR8-Svneo cell proliferation. Methods The proliferation of HTR8-Svneo cells cultured in vitro under diverse concentrations (0, 10, 50, 100, 250 and 500 ng/mL) of leptin was tested by means of MTT. Then, HTR8-Svneo cells were treated with leptin (0, 100 and 500 ng/mL), and leptin receptor (leptin R) profiling, ID mRNA profiling and the level of the ID2 protein were detected by RT-PCR, real-time PCR and Western blot, respectively. To observe the effect of leptin on HTR8-Svneo cell proliferation after ID2-siRNA treatment, HTR8-Svneo cells were divided into 6 groups; leptin(0 ng/mL) , leptin( 100 ng/mL) , leptin(500 ng/mL) , leptin(0 ng/mL) +ID2-siRNA, leptin( 100 ng/mL) + ID2-siRNA and leptin(500 ng/mL) + ID2-siRNA. Results Proliferation of HTR8-Svneo cells was enhanced by high concentrations of leptin (100, 250 and 500 ng/mL) compared with leptin (0 ng/mL) (P<0.05). Leptin receptor subtypes except HuB219. 2 were detected on HTR8-Svneo cells. The up-regulation of ID2 or OB-RL was induced by leptin in a dose-dependent manner. ID2-siRNA inhibited expression of the ID2 protein and reversed the enhancement of HTR8-Svneo cell proliferation induced by leptin. Conclusion Leptin enhances HTR8-Svneo cell proliferation by the pathway of leptin R-ID2, which offers new basal data for elucidating the regulatory mechanism of proliferation of tro-phocytes.%目的 研究瘦素(Leptin)对HTR8-SVneo滋养细胞系增殖的影响及对DNA结合和分化抑制剂(ID)分子表达水平的影响,探讨Leptin对HTR8-SVneo细胞增殖作用的调节机制.方法 体外培养HTR8-SVneo滋养细胞,MTT法检测Leptin不同质量浓度(0、10、50、100、250、500 ng/mL)刺激后细胞增殖情况.HTR8-SVneo细胞培养体系中加入Leptin(0、100、500 mg/m

  11. The ATWS analysis of one control rod withdraw out of the HTR-10GT core in addition with bypass valve failure

    Energy Technology Data Exchange (ETDEWEB)

    Lang, Minggang, E-mail: langmg@tsinghua.edu.cn [Institute of Nuclear and New Energy Technology, Tsinghua University, Room 207, Building B, Nengkelou, Beijing (China); Dong, Yujie [Institute of Nuclear and New Energy Technology, Tsinghua University, Room 503, Building A, Nengkelou, Beijing (China)

    2014-05-01

    The 10 MW high temperature gas cooled test reactor (HTR-10) has been built in Institute of Nuclear and New Energy Technology (INET) and has been operating successfully since the beginning of 2003. The core outlet temperature of HTR-10 is 700 °C. To verify the technology of gas-turbine direct cycle, at first INET had a plan to increase its core outlet temperature to 750 °C and use a helium gas turbine instead of the steam generator (then the reactor is called HTR-10GT). Though HTR-10 has good intrinsic safety, the design basic accidents and beyond design basic accidents of HTR10-GT must be analyzed according to China's nuclear regulations due to changed operation parameters. THERMIX code system is used to study the ATWS accident of one control rod withdrawal out of the core by a mistake. After a control rod in the side reflector was withdrawn out at a speed of 1 cm/s by a mistake, a positive reactivity was inserted and the reactor power increased and the temperature of the core increased. When the neutron flux of power measuring range exceeded 123% and the core outlet temperature was greater than 800 °C, the reactor should scram. It was supposed that all the control rods in the reflectors had been blocked and the reactor could not scram. Thus the accident went on and the core temperature and the system pressure increased but the reactor shutdown at last because of its natural negative temperature reactivity feedback mechanism, in spite of the failure of bypass valve. The residual heat would be removed out of the core by the cavity cooling system. During the accident sequence the maximum fuel temperature was 1283.3 °C. It was a litter than 1230 °C – the fuel temperature limitation of HTR-10. Now the sphere fuel used in HTR-10GT will also be used in HTR-PM and the temperature limitation will be raised to 1620 °C, so the HTR-10GT is safe during the ATWS of one control rod withdrawal out of the core. The paper also compares the analysis result of HTR10-GT

  12. Re-examination of the role of the Brucella melitensis HtrA stress response protease in virulence in pregnant goats.

    Science.gov (United States)

    Roop, R M; Phillips, R W; Hagius, S; Walker, J V; Booth, N J; Fulton, W T; Edmonds, M D; Elzer, P H

    2001-09-03

    Based on previously reported studies describing the experimental infection of pregnant goats with B. melitensis strain RWP5, we proposed that the HtrA protease plays an important role in the virulence of B. melitensis in its natural ruminant host. Subsequent studies, however, have shown that RWP5 is actually an htrA cycL double mutant. In order to definitively evaluate the role of the B. melitensis htrA in virulence, we constructed an authentic htrA mutant and examined this strain in pregnant goats. The findings of these studies indicate that the contribution of the htrA gene product to the virulence of B. melitensis in its natural host is not as great as was previously proposed.

  13. Costs of head-end incineration with respect to Kr separation in the reprocessing of HTR fuel elements

    Energy Technology Data Exchange (ETDEWEB)

    Barnert-Wiemer, H.; Boehnert, R.

    1976-07-15

    The C-incinerations and the Kr-separations during head-end incineration in the reprocessing of HTR fuel elements are described. The costs for constructing an operating a head-end incineration of reprocessing capacities with 5,000 to 50,000 MW(e)-HTR power have been determined. The cost estimates are divided into investment and operating costs, further after the fraction of the N/sub 2/-content in the incineration exhaust gas, which strongly affects costs. It appears that, in the case of Kr-separation from the incineration exhaust gas, the investment costs as well as the operating costs of the head-end for N/sub 2/-containing exhaust gas are considerably greater than those for gas without N/sub 2/. The C-incineration of the graphite of the HTR fuel elements should therefore only be performed with influx gas that is free of N/sub 2/.

  14. Uncertainty and sensitivity analysis of filling fraction of pebble bed in pebble bed HTR

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Hao, E-mail: haochen.heu@163.com [Fundamental Science on Nuclear Safety and Simulation Technology Laboratory, College of Nuclear Science and Technology, Harbin Engineering University, Harbin (China); Institute of Nuclear and New Energy Technology (INET), Collaborative Innovation Center of Advanced Nuclear Energy Technology, Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education, Tsinghua University, Beijing (China); Fu, Li; Jiong, Guo; Lidong, Wang [Institute of Nuclear and New Energy Technology (INET), Collaborative Innovation Center of Advanced Nuclear Energy Technology, Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education, Tsinghua University, Beijing (China)

    2015-10-15

    Highlights: • An analysis approach is proposed to conduct SAU analysis of filling fraction of pebble bed core. • The contribution of uncertainty in filling fraction to key parameters of pebble bed core is quantified. • The primary drivers of the uncertainty in the key parameters are identified by sensitivity analysis. • Mechanism of effect of uncertainty in the filling fraction to key parameters is studied in depth. - Abstract: The filling fraction of pebble bed in each small region has some uncertainty, which will contribute to the total uncertainty in the key parameters of pebble bed core, such as power peak, axial offset, k{sub eff} and so on. In fact, the heavy metal content and graphite content of the corresponding region will change synchronously due to the perturbation of filling fraction but the ratio of atomic number density of moderator to the fuel (carbon–uranium) is still constant. To investigate these effects, the Chinese demonstration plant HTR-PM was selected as the research object and the VSOP code and CUSA package were used to conduct detailed analysis of the influence of the uncertainty in the filling fraction on the HTR-PM output variables of interest, based on the propagation of input uncertainties by using statistical sampling method to calculate uncertainty and sensitivity information from the simulation results. At the same time, uncertainty and sensitivity analysis of uranium loading had also been conducted for comparative analysis to study the mechanism of effect of uncertainty in the filling fraction to key parameters, and therefore only the heavy metal content of the corresponding region changes in the presence of perturbation of uranium loading. Finally, the propagated uncertainty in the power peak, axial offset and k{sub eff} of HTR-PM core was obtained and the primary drivers of the uncertainty in the key parameters were identified by sensitivity analysis.

  15. Burning minor actinides in a HTR energy spectrum and effects on the final radiotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Pohl, Christoph, E-mail: christoph.pohl@de.tuv.com [Forschungszentrum Juelich GmbH, 52425 Juelich (Germany); Allelein, Hans-Josef [Forschungszentrum Juelich GmbH, 52425 Juelich (Germany)

    2012-10-15

    The production of nuclear energy with existing nuclear reactors is equivalent to the use of low enriched uranium. But the neutron capture of the large corresponding U-238 fuel fraction also generates a build-up of plutonium isotopes and minor actinides as Neptunium, Americium and Curium. These actinides are dominant for the long time assessment of final disposal therefore a minimization of the long living isotopes is aspired. Burning the actinides in a high temperature helium cooled graphite moderated reactor (HTR) is one of these options. Using plutonium isotopes to sustain the criticality of the system is intended to avoid highly enriched uranium because of international regulations and low enriched uranium because of the build up of new actinides from neutron capture in U-238. Also fractions of plutonium isotopes are build up to minor actinides but for this absorption the overall number of actinides keeps constant. Nevertheless for the final assessment the activity and toxicity of all important actinides have to be taken into account. This paper comprises calculations for plutonium/minor actinides/thorium fuel compositions, their correlated final burn-up and the long term activity and toxicity for a generic pebble bed HTR based on the reference design of the 400 MW PBMR. In particular the behaviour of the different minor actinide isotopes in the higher thermal energy spectrum of a HTR will be discussed. Thorium based fuel - as a promising alternative to uranium based fuel - offers several advantages as a minimized build up of new Pu and MA, a higher thermal conductivity and melting point. Combining the thorium fuel with a significant fraction of minor actinides and an isotope fraction consistent with burned LWR fuel the total amount of the minor actinides stays nearly unchanged while the isotope composition significantly changes. This behaviour with respect to the initial heavy metal load and the influence on the long term activity and toxicity will be discussed.

  16. Pilot study of dynamic Bayesian networks approach for fault diagnostics and accident progression prediction in HTR-PM

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Yunfei; Tong, Jiejuan; Zhang, Liguo, E-mail: lgzhang@tsinghua.edu.cn; Zhang, Qin

    2015-09-15

    Highlights: • Dynamic Bayesian network is used to diagnose and predict accident progress in HTR-PM. • Dynamic Bayesian network model of HTR-PM is built based on detailed system analysis. • LOCA Simulations validate the above model even if part monitors are lost or false. - Abstract: The first high-temperature-reactor pebble-bed demonstration module (HTR-PM) is under construction currently in China. At the same time, development of a system that is used to support nuclear emergency response is in progress. The supporting system is expected to complete two tasks. The first one is diagnostics of the fault in the reactor based on abnormal sensor measurements obtained. The second one is prognostic of the accident progression based on sensor measurements obtained and operator actions. Both tasks will provide valuable guidance for emergency staff to take appropriate protective actions. Traditional method for the two tasks relies heavily on expert judgment, and has been proven to be inappropriate in some cases, such as Three Mile Island accident. To better perform the two tasks, dynamic Bayesian networks (DBN) is introduced in this paper and a pilot study based on the approach is carried out. DBN is advantageous in representing complex dynamic systems and taking full consideration of evidences obtained to perform diagnostics and prognostics. Pearl's loopy belief propagation (LBP) algorithm is recommended for diagnostics and prognostics in DBN. The DBN model of HTR-PM is created based on detailed system analysis and accident progression analysis. A small break loss of coolant accident (SBLOCA) is selected to illustrate the application of the DBN model of HTR-PM in fault diagnostics (FD) and accident progression prognostics (APP). Several advantages of DBN approach compared with other techniques are discussed. The pilot study lays the foundation for developing the nuclear emergency response supporting system (NERSS) for HTR-PM.

  17. Modulation of mitochondrial function and morphology by interaction of Omi/HtrA2 with the mitochondrial fusion factor OPA1

    Energy Technology Data Exchange (ETDEWEB)

    Kieper, Nicole; Holmstroem, Kira M.; Ciceri, Dalila; Fiesel, Fabienne C. [Center of Neurology and Hertie Institute for Clinical Brain Research, 72076 Tuebingen (Germany); Wolburg, Hartwig [Institute of Pathology, University of Tuebingen, 72076 Tuebingen (Germany); Ziviani, Elena; Whitworth, Alexander J. [Medical Research Council Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN (United Kingdom); Martins, L. Miguel [Cell Death Regulation Laboratory, MRC Toxicology Unit, Leicester LE1 9HN (United Kingdom); Kahle, Philipp J., E-mail: philipp.kahle@uni-tuebingen.de [Center of Neurology and Hertie Institute for Clinical Brain Research, 72076 Tuebingen (Germany); Krueger, Rejko, E-mail: rejko.krueger@uni-tuebingen.de [Center of Neurology and Hertie Institute for Clinical Brain Research, 72076 Tuebingen (Germany)

    2010-04-15

    Loss of Omi/HtrA2 function leads to nerve cell loss in mouse models and has been linked to neurodegeneration in Parkinson's and Huntington's disease. Omi/HtrA2 is a serine protease released as a pro-apoptotic factor from the mitochondrial intermembrane space into the cytosol. Under physiological conditions, Omi/HtrA2 is thought to be involved in protection against cellular stress, but the cytological and molecular mechanisms are not clear. Omi/HtrA2 deficiency caused an accumulation of reactive oxygen species and reduced mitochondrial membrane potential. In Omi/HtrA2 knockout mouse embryonic fibroblasts, as well as in Omi/HtrA2 silenced human HeLa cells and Drosophila S2R+ cells, we found elongated mitochondria by live cell imaging. Electron microscopy confirmed the mitochondrial morphology alterations and showed abnormal cristae structure. Examining the levels of proteins involved in mitochondrial fusion, we found a selective up-regulation of more soluble OPA1 protein. Complementation of knockout cells with wild-type Omi/HtrA2 but not with the protease mutant [S306A]Omi/HtrA2 reversed the mitochondrial elongation phenotype and OPA1 alterations. Finally, co-immunoprecipitation showed direct interaction of Omi/HtrA2 with endogenous OPA1. Thus, we show for the first time a direct effect of loss of Omi/HtrA2 on mitochondrial morphology and demonstrate a novel role of this mitochondrial serine protease in the modulation of OPA1. Our results underscore a critical role of impaired mitochondrial dynamics in neurodegenerative disorders.

  18. Theoretical Analysis of Effective Thermal Conductivity for the Chinese HTR-PM Heat Transfer Test Facility

    Directory of Open Access Journals (Sweden)

    Cheng Ren

    2017-01-01

    Full Text Available The Chinese high temperature gas-cooled reactor pebble bed module (HTR-PM demonstration project has attracted increasing attention. In order to support the project, a large-scale heat transfer test facility has been constructed for pebble bed effective thermal conductivity measurement over the whole temperature range (0~1600 °C. Based on different heat transfer mechanisms in the randomly packed pebble bed, three different types of effective thermal conductivity have been theoretically evaluated. A prediction of the total effective thermal conductivity of the pebble bed over the whole temperature range is provided for the optimization of the test facility and guidance of further experiments.

  19. HTR combustion head end comparison of the shaft furnace and fluidized bed processes

    Energy Technology Data Exchange (ETDEWEB)

    Boehnert, R.; Kaiser, G.; Pirk, H.; Tillessen, U.

    1975-01-15

    Two methods are described for the combustion of the graphite of HTR fuel elements, a sufficient description of the principles being given to permit an understanding of the processes. The present state of the technology of the two processes is then compared on the basis of the results obtained at Gulf General Atomic. Finally, the possibilities of further development are examined using a pilot plant designed to deliver a reactor power of 7000 MWe as the basis. The present report is a collection of facts. It contains neither an evaluation nor a recommendation. A summarized comparison of the state of the technology and the possibilities of development is given in tabular form.

  20. Checking calculation of the steady state temperature field of HTR-10

    Energy Technology Data Exchange (ETDEWEB)

    Yujie Dong; Zuying Gao [Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084 (China)

    2005-07-01

    Full text of publication follows: Modular high temperature gas-cooled reactor (HTR), to which has lately been paid much attention by international community of nuclear energy, is a type of promising advanced reactor for power generation and process heat production. A 10 MW test module (HTR-10) has been designed, constructed and operated by the Institute of Nuclear and New Energy Technology (INET). It is a helium-cooled, graphite-moderated, pebble-bed type and reached the first criticality in December 2000 and full power operation in January 2003. The reactor consists of reactor pressure vessel (RPV), internal graphite components and carbon bricks, metallic components, fuel elements, control rods and their driven mechanisms, small absorber ball shut-down system, fuel charging and discharging systems, etc. Internal metallic components include core vessel, bottom support plate, top support plate, etc. During construction of this reactor, a number of thermocouples had been fixed in the side reflector, top reflector and bottom reflector, carbon brick thermal insulator, core vessel and pressure vessel. The temperatures at corresponding points were recorded when the reactor was operating. The measurement results depicted the general situation of temperature distribution within the reactor. In order to check the design validity and verify the analysis program, a checking calculation of the steady state temperature field of the reactor has been done. The calculation program is THERMIX, which was used in the thermal-hydraulic design of the HTR-10. An R-Z coordinate was used. Helium bypass flows and leakage via clearances among graphite components were simulated. Heat generation rate distribution used in this calculation is obtained from the calculated results of VSOP code, which is a special purpose program for pebble bed reactor physics. The temperatures calculated include temperature distribution within the pebble bed, solid material temperatures and the maximum

  1. The effect of IL-6 on the trophoblast cell line HTR-8/SVneo

    Directory of Open Access Journals (Sweden)

    Jovanović Milica

    2010-01-01

    Full Text Available Embryonic development up to the blastocyst stage, implantation into the uterine wall and the development of the functional placenta are steps crucial for the establishment of normal pregnancy. Specific cells of the placenta, the trophoblast cells, invade the uterine stroma and spiral arteries, adapting them to pregnancy. Interleukin-6 is present in the human endometrium during the receptive phase and early pregnancy. Trophoblasts also produce IL-6, which was found to stimulate trophoblast invasion and migration in vitro. Here we show that the activity of MMP-9 may contribute to the observed increased invasion. In addition, in the HTR-8/SVneo trophoblast cell line IL-6 increases cell proliferation. .

  2. Needs in Research and Development on materials for the gas coolant nuclear system: HTR/VHTR and GFR; Besoins en R et D sur les materiaux pour les systemes nucleaires a caloporteur gaz: HTR/VHTR et GFR

    Energy Technology Data Exchange (ETDEWEB)

    Billot, Ph. [CEA Saclay, Dir. du Developpement et de l' Innovation Nucleares (DEN/DDIN), 91 - Gif Sur Yvette (France)

    2003-07-01

    This presentation takes stock on the materials for high temperature reactors HTR (850 C), very high temperature VHTR(>1000 C) and fast neutrons high temperature GGF(850 C). It concerns the welding materials for the vessel, Ni-based superalloys for gas turbines, coatings, graphite, ceramics and corrosion studies. (A.L.B.)

  3. A SMALL MODULAR REACTOR DESIGN FOR MULTIPLE ENERGY APPLICATIONS: HTR50S

    Directory of Open Access Journals (Sweden)

    X. YAN

    2013-06-01

    Full Text Available HTR50S is a small modular reactor system based on HTGR. It is designed for a triad of applications to be implemented in successive stages. In the first stage, a base plant for heat and power is constructed of the fuel proven in JAEA's 950°C, 30MWt test reactor HTTR and a conventional steam turbine to minimize development risk. While the outlet temperature is lowered to 750°C for the steam turbine, thermal power is raised to 50MWt by enabling 40% greater power density in 20% taller core than the HTTR. However the fuel temperature limit and reactor pressure vessel diameter are kept. In second stage, a new fuel that is currently under development at JAEA will allow the core outlet temperature to be raised to 900°C for the purpose of demonstrating more efficient gas turbine power generation and high temperature heat supply. The third stage adds a demonstration of nuclear-heated hydrogen production by a thermochemical process. A licensing approach to coupling high temperature industrial process to nuclear reactor will be developed. The low initial risk and the high longer-term potential for performance expansion attract development of the HTR50S as a multipurpose industrial or distributed energy source.

  4. Benchmark Evaluation of the HTR-PROTEUS Absorber Rod Worths (Core 4)

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess; Leland M. Montierth

    2014-06-01

    PROTEUS was a zero-power research reactor at the Paul Scherrer Institute (PSI) in Switzerland. The critical assembly was constructed from a large graphite annulus surrounding a central cylindrical cavity. Various experimental programs were investigated in PROTEUS; during the years 1992 through 1996, it was configured as a pebble-bed reactor and designated HTR-PROTEUS. Various critical configurations were assembled with each accompanied by an assortment of reactor physics experiments including differential and integral absorber rod measurements, kinetics, reaction rate distributions, water ingress effects, and small sample reactivity effects [1]. Four benchmark reports were previously prepared and included in the March 2013 edition of the International Handbook of Evaluated Reactor Physics Benchmark Experiments (IRPhEP Handbook) [2] evaluating eleven critical configurations. A summary of that effort was previously provided [3] and an analysis of absorber rod worth measurements for Cores 9 and 10 have been performed prior to this analysis and included in PROTEUS-GCR-EXP-004 [4]. In the current benchmark effort, absorber rod worths measured for Core Configuration 4, which was the only core with a randomly-packed pebble loading, have been evaluated for inclusion as a revision to the HTR-PROTEUS benchmark report PROTEUS-GCR-EXP-002.

  5. 萨能奶山羊乳腺组织5-HTR2基因的克隆与序列分析%Molecular Cloning and Sequence Analysis of the 5-HTR2 Gene of Sanen Breast Milk Goat

    Institute of Scientific and Technical Information of China (English)

    江青东; 李新平

    2011-01-01

    以容易获取并具代表性的乳腺组织——奶山羊乳腺为载体,进行哺乳动物乳腺组织中5-HTR2基因的克隆,为下一步研究奶牛乳腺组织中5-HTR基因的分布及生物学特性提供理论基础.根据GenBank其他物种5- HT受体- 2(5 - HTR2)基因序列的同源序列,对奶山羊5-HTR2基因设计1对克隆引物,从奶山羊乳腺组织提取总RNA进行RT - PCR,将PCR产物克隆、测序.结果显示,奶山羊5 - HTR2基因的ORF与人(NM-001165947)、鼠(NM-017254)和狗(AB193092)的序列同源性分别为87.2%、93.5%及78.9%,推测其氨基酸序列与人和鼠在基因进化方面,羊和鼠的同源性最高,其次是羊与人.奶山羊5 - HTR2基因全序列与鼠和人的同源性分别为87%和75%,相似度分别为62%和82%;基因氨基酸序列信号肽均为1~19 aa,SapB结构域均为53~121aa,结构特征与人、鼠的5- HTR2结构特征相一致.%To provide theoretical basis for further study on bovine mammary tissue distribution of 5-HTR genes and biological characteristics, the 5-HT receptor-2 (5-HTR2) gene was cloned from the breast tissue of breast milk goats by RT-PCR and the PCR products were cloned and se-quenced. The results showed that the homology of the ORF of dairy goat 5-HTR2 gene to that of human (NM-001165947), rat (NM-017254), and dog (AB193092) were 87. 2%, 93. 5%,and 78. 9 %, respectively. The homology of goat 5-HTR2 gene sequences to that of the mouse and human were 87% and 75%,respectively. The amino acid sequence of the signal peptide is from 1 to 19aa and the SapB domain if from 53 to 121 aa,with the similar structures to that of human and rat.

  6. Dependence of the thermoluminescent high-temperature ratio (HTR) of LiF:Mg,Ti detectors on proton energy and dose

    CERN Document Server

    Bilski, P; Swakon, J; Weber, A

    2014-01-01

    The high-temperature ratio (HTR) is a parameter quantifying changes of the shape of the high-temperature part of the LiF:Mg,Ti glow-curve after exposure to densely ionizing radiation. It was introduced in order to estimate the effective LET of an unknown radiation field and to correct the decreased relative TL efficiency for high Linear Energy Transfer (LET) radiation. In the present work the dependence of HTR on proton energy (14.5 to 58 MeV) and dose (0.5 to 30 Gy) was investigated. All measured HTR values were at the level of 1.2 or higher, therefore significantly different from the respective value for gamma rays (HTR is equal to 1), but HTR was found to be insensitive to changes of proton energy above 20 MeV. As a result the relationship between HTR and relative TL efficiency is not unequivocal. The HTR was found to be dependent on absorbed dose even for the lowest studied doses.

  7. Westinghouse experiences with HTR as the basis for design of the New Generation of Nuclear Power Plants (NGNP); Experiencias de Estinghouse con el HTR como Base para el diseno de la Nueva Generacion de Centrales Nucleares (BGNP)

    Energy Technology Data Exchange (ETDEWEB)

    Schoning, J.; Esch, M.; Knoche, D.; Freis, D.; Finken, H.; Drifhout, F.

    2010-07-01

    For more than three decades Germany had a very ambitious High Temperature Reactor (HTR) program which included numerous research activities and the construction and operation of two HTRs. The whole program had a volume of more than 6 billion D-Marks and covered activities of industry, research centres and universities. Within this program the physical feasibility of a pebble bed HTR was power for the first time in the Arbeitsgemeinschaft Versuchsreaktor (AVR) research reactor at Research Centre Julich (FZJ). Later it served as a test bed for new developed fuel as well as test reactor for numerous successful experiments on the inherent safety of this special type of nuclear reactor. The subsequent power plant THTR-300 with a rated electrical power of 300 MWel at Hamm-Uentrop was constructed as a demonstration plant. With THTR-300 the feasibility of a large commercial pebble bed reactor was demonstrated. Both reactors were built in the state of North-Rhine Westphalia with its traditional resources of coal and its heavy and chemical industry. HTRs were specifically meant to provide process heat to these industries and with the Project Nuclear Process Heat (PNP) a plant was developed to serve this means. Based on this HTR specific expertise and on actual experience from AP1000TM development and construction, Westinghouse has the overall expertise in house to design a generation IV reactor system in the near term future. On HTR specific systems and components the maturity of the technology was demonstrated with THTR-300. Potential design approaches for future HTR concepts for process heat generation are discussed. (Author) 1 refs.

  8. Gadd45α expression in preeclampsia placenta and the effect of Gadd45α on trophoblast HTR8/Svneo

    Institute of Scientific and Technical Information of China (English)

    Li Wang; Lin Zhao

    2016-01-01

    Objective:To study the expression of Gadd45α in preeclampsia placenta and the regulating effect of Gadd45 knockdown on trophoblast HTR8/Svneo.Methods:Preeclampsia placenta tissue and normal placenta tissue were collected, and mRNA contents and protein contents of Gadd45α were detected by fluorescent quantitative PCR and Western blotting respectively; trophoblast cells HTR8/Svneo were cultured and after transfection of Gadd45αsiRNA, cell invasion ability and expression of invasion-assiotiated molecules were detected. Results:mRNA content and protein content of Gadd45α in preeclampsia placenta tissue were higher than those in normal placenta tissue; after transfection of Gadd45α siRNA, mRNA content and protein content of Gadd45α in HTR8/Svneo cells significantly decreased, and the number of invasive cells as well as expression of MMP1, MMP2, MMP3 and MMP9 significantly increased. Conclusion: The expression of Gadd45α in preeclampsia placenta abnormally increases; inhibting the expression of Gadd45α in trophoblasts HTR8/Svneo can promote invasion and increase the expression of MMPs molecules.

  9. The NG2 Proteoglycan Protects Oligodendrocyte Precursor Cells against Oxidative Stress via Interaction with OMI/HtrA2.

    Directory of Open Access Journals (Sweden)

    Frank Maus

    Full Text Available The NG2 proteoglycan is characteristically expressed by oligodendrocyte progenitor cells (OPC and also by aggressive brain tumours highly resistant to chemo- and radiation therapy. Oligodendrocyte-lineage cells are particularly sensitive to stress resulting in cell death in white matter after hypoxic or ischemic insults of premature infants and destruction of OPC in some types of Multiple Sclerosis lesions. Here we show that the NG2 proteoglycan binds OMI/HtrA2, a mitochondrial serine protease which is released from damaged mitochondria into the cytosol in response to stress. In the cytosol, OMI/HtrA2 initiates apoptosis by proteolytic degradation of anti-apoptotic factors. OPC in which NG2 has been downregulated by siRNA, or OPC from the NG2-knockout mouse show an increased sensitivity to oxidative stress evidenced by increased cell death. The proapoptotic protease activity of OMI/HtrA2 in the cytosol can be reduced by the interaction with NG2. Human glioma expressing high levels of NG2 are less sensitive to oxidative stress than those with lower NG2 expression and reducing NG2 expression by siRNA increases cell death in response to oxidative stress. Binding of NG2 to OMI/HtrA2 may thus help protect cells against oxidative stress-induced cell death. This interaction is likely to contribute to the high chemo- and radioresistance of glioma.

  10. Pre-economic analysis of HTR in preparation for a comprehensive economic assessment of HTRs in the world

    Energy Technology Data Exchange (ETDEWEB)

    Bredimas, Alexandre, E-mail: alexandre.bredimas@strane-innovation.com

    2014-05-01

    High temperature nuclear reactors will address mainly the industrial cogeneration market and compete with gas cogeneration, the current reference technology. The key question for HTR is therefore: how far are HTRs competitive against gas technologies? This simple question demands a complex response. First, the cogeneration scheme has to be discussed according the specificities in heat usage of every industry as they will impact the design. Second, the costs, revenues and risks of the different lifecycle phases for both a HTR and gas cogeneration plant have to be assessed and compared. These parameters will greatly depend on each location (personnel costs, gas local prices, CO{sub 2} pricing, etc.). A particular attention has to be given to the risk interactions between the cogeneration plant and the industrial facility it is supplying with heat and electricity (e.g. tritium contamination in industrial processes, explosion of flammable products in industrial site). This paper aims mainly at starting exchanges at international level with other equivalent initiatives in order to assess in general terms the economic viability of HTR worldwide, in relation to the evaluation of the HTR global market.

  11. Association between HTR2C gene polymorphisms and the metabolic syndrome in patients Using Antipsychotics: A Replication Study

    NARCIS (Netherlands)

    Risselada, Arne; Vehof, Jelle; Bruggeman, Richard; Wilffert, Bob; Cohen, Dan; Al Hadithy, Asmar; Arends, Johan; Mulder, Hans

    2010-01-01

    Background: In two previous studies we investigated the association between the rs1414334 C/G and 759 C/T polymorphisms in the HTR2C gene, coding for the 5HT2c-receptor, and prevalence of the metabolic syndrome in a schizophrenic population. In both studies we found an association between the varian

  12. Association between HTR2C polymorphisms and the metabolic syndrome in patients using antipsychotics : a replication study

    NARCIS (Netherlands)

    Risselada, A.; Vehof, J.; Bruggeman, R.; Wilffert, B.; Cohen, D.; Al Hadithy, A. F.; Arends, J.; Mulder, H.

    2010-01-01

    Background: In two previous studies we investigated the possible association between the rs1414334 C/G and 759 C/T polymorphisms in the gene that encodes the 5HT2c receptor (HTR2c) and prevalence of the metabolic syndrome in a schizophrenic population [1,2]. In both studies we found an association b

  13. Association between HTR2C polymorphisms and the metabolic syndrome in patients using antipsychotics: A replication study

    NARCIS (Netherlands)

    Risselada, A.; Vehof, J.; Bruggeman, R.; Wilffert, B.; Cohen, D.; Al Hadithy, A.F.; Arends, J.; Mulder, H.

    2010-01-01

    Background: In two previous studies we investigated the possible association between the rs1414334 C/G and 759 C/T polymorphisms in the gene that encodes the 5HT2c receptor (HTR2c) and prevalence of the metabolic syndrome in a schizophrenic population [1,2]. In both studies we found an association b

  14. The association between HTR2C polymorphisms and obesity in psychiatric patients using antipsychotics: a cross-sectional study.

    NARCIS (Netherlands)

    Mulder, H.; Franke, B.; Beek, A.A. van der; Arends, J.; Wilmink, F.W.; Egberts, A.C.G.; Scheffer, H.

    2007-01-01

    The use of antipsychotics is associated with an increased risk of obesity. This consideration makes it important to search for determinants that can predict the risk for antipsychotic-induced obesity. In this cross-sectional study, we investigated whether polymorphisms in the HTR2C gene were associa

  15. Association between HTR2C gene polymorphisms and the metabolic syndrome in patients using antipsychotics : a replication study

    NARCIS (Netherlands)

    Risselada, A. J.; Vehof, J.; Bruggeman, R.; Wilffert, B.; Cohen, D.; Al Hadithy, A. F.; Arends, J.; Mulder, H.

    2012-01-01

    In two previous studies we found an association between HTR2C polymorphisms and the prevalence of the metabolic syndrome in patients using antipsychotics. In this study, we set out to replicate our findings in a third separate sample of patients. Data for this cross-sectional study came from the ong

  16. A genetic variant of HTR2C may play a role in the manifestation of Tourette syndrome.

    Science.gov (United States)

    Dehning, Sandra; Müller, Norbert; Matz, Judith; Bender, Andreas; Kerle, Irina; Benninghoff, Jens; Musil, Richard; Spellmann, Ilja; Bondy, Brigitta; Möller, Hans-Juergen; Riedel, Michael; Zill, Peter

    2010-02-01

    Gilles de la Tourette syndrome (GTS) (MIM 137580) is a complex neuropsychiatric disorder probably originating from a disturbed interplay of several neurotransmitter systems in the prefrontal-limbic-basal ganglia loop. Polygenetic multifactorial inheritance has been postulated; nevertheless, no confirmed susceptible genes have been identified yet. As neuroimaging studies allude to dopaminergic and serotonergic dysfunction in GTS and serotonin as an important factor for dopamine release, genotyping of common polymorphisms in the serotonergic receptor (HTR1A: C-1019G; HTR2A: T102C, His452Tyr, A-1438G; HTR2C: C-759T, G-697C) and transporter genes (SLC6A4) was carried out in 87 patients with GTS, compared with 311 matched controls. We found a nominally significant association between both polymorphisms in the HTR2C and the GTS, which was more pronounced in male patients. Analysis of the further serotonergic polymorphisms did not reveal any significant result. A modified function of these promoter polymorphisms may contribute to the complex interplay of serotonin and dopamine and then to the manifestation of GTS.

  17. Association between HTR2C gene polymorphisms and the metabolic syndrome in patients using antipsychotics : a replication study

    NARCIS (Netherlands)

    Risselada, A. J.; Vehof, J.; Bruggeman, R.; Wilffert, B.; Cohen, D.; Al Hadithy, A. F.; Arends, J.; Mulder, H.

    In two previous studies we found an association between HTR2C polymorphisms and the prevalence of the metabolic syndrome in patients using antipsychotics. In this study, we set out to replicate our findings in a third separate sample of patients. Data for this cross-sectional study came from the

  18. Association between HTR2C polymorphisms and the metabolic syndrome in patients using antipsychotics : a replication study

    NARCIS (Netherlands)

    Risselada, A.; Vehof, J.; Bruggeman, R.; Wilffert, B.; Cohen, D.; Al Hadithy, A. F.; Arends, J.; Mulder, H.

    2010-01-01

    Background: In two previous studies we investigated the possible association between the rs1414334 C/G and 759 C/T polymorphisms in the gene that encodes the 5HT2c receptor (HTR2c) and prevalence of the metabolic syndrome in a schizophrenic population [1,2]. In both studies we found an association b

  19. Association between HTR2C polymorphisms and the metabolic syndrome in patients using antipsychotics: A replication study

    NARCIS (Netherlands)

    Risselada, A.; Vehof, J.; Bruggeman, R.; Wilffert, B.; Cohen, D.; Al Hadithy, A.F.; Arends, J.; Mulder, H.

    2010-01-01

    Background: In two previous studies we investigated the possible association between the rs1414334 C/G and 759 C/T polymorphisms in the gene that encodes the 5HT2c receptor (HTR2c) and prevalence of the metabolic syndrome in a schizophrenic population [1,2]. In both studies we found an association b

  20. Association between HTR2C gene polymorphisms and the metabolic syndrome in patients Using Antipsychotics: A Replication Study

    NARCIS (Netherlands)

    Risselada, Arne; Vehof, Jelle; Bruggeman, Richard; Wilffert, Bob; Cohen, Dan; Al Hadithy, Asmar; Arends, Johan; Mulder, Hans

    2010-01-01

    Background: In two previous studies we investigated the association between the rs1414334 C/G and 759 C/T polymorphisms in the HTR2C gene, coding for the 5HT2c-receptor, and prevalence of the metabolic syndrome in a schizophrenic population. In both studies we found an association between the varian

  1. mRNA Expression and DNA Methylation Analysis of Serotonin Receptor 2A (HTR2A in the Human Schizophrenic Brain

    Directory of Open Access Journals (Sweden)

    Sern-Yih Cheah

    2017-01-01

    Full Text Available Serotonin receptor 2A (HTR2A is an important signalling factor implicated in cognitive functions and known to be associated with schizophrenia. The biological significance of HTR2A in schizophrenia remains unclear as molecular analyses including genetic association, mRNA expression and methylation studies have reported inconsistent results. In this study, we examine HTR2A expression and methylation and the interaction with HTR2A polymorphisms to identify their biological significance in schizophrenia. Subjects included 25 schizophrenia and 25 control post-mortem brain samples. Genotype and mRNA data was generated by transcriptome sequencing. DNA methylation profiles were generated for CpG sites within promoter-exon I region. Expression, genotype and methylation data were examined for association with schizophrenia. HTR2A mRNA levels were reduced by 14% (p = 0.006 in schizophrenia compared to controls. Three CpG sites were hypermethylated in schizophrenia (cg5 p = 0.028, cg7 p = 0.021, cg10 p = 0.017 and HTR2A polymorphisms rs6314 (p = 0.008 and rs6313 (p = 0.026 showed genetic association with schizophrenia. Differential DNA methylation was associated with rs6314 and rs6313. There was a strong correlation between HTR2A DNA methylation and mRNA expression. The results were nominally significant but did not survive the rigorous Benjamini-Hochberg correction for multiple testing. Differential HTR2A expression in schizophrenia in our study may be the result of the combined effect of multiple differentially methylated CpG sites. Epigenetic HTR2A regulation may alter brain function, which contributes to the development of schizophrenia.

  2. mRNA Expression and DNA Methylation Analysis of Serotonin Receptor 2A (HTR2A) in the Human Schizophrenic Brain.

    Science.gov (United States)

    Cheah, Sern-Yih; Lawford, Bruce R; Young, Ross McD; Morris, Charles P; Voisey, Joanne

    2017-01-04

    Serotonin receptor 2A (HTR2A) is an important signalling factor implicated in cognitive functions and known to be associated with schizophrenia. The biological significance of HTR2A in schizophrenia remains unclear as molecular analyses including genetic association, mRNA expression and methylation studies have reported inconsistent results. In this study, we examine HTR2A expression and methylation and the interaction with HTR2A polymorphisms to identify their biological significance in schizophrenia. Subjects included 25 schizophrenia and 25 control post-mortem brain samples. Genotype and mRNA data was generated by transcriptome sequencing. DNA methylation profiles were generated for CpG sites within promoter-exon I region. Expression, genotype and methylation data were examined for association with schizophrenia. HTR2A mRNA levels were reduced by 14% (p = 0.006) in schizophrenia compared to controls. Three CpG sites were hypermethylated in schizophrenia (cg5 p = 0.028, cg7 p = 0.021, cg10 p = 0.017) and HTR2A polymorphisms rs6314 (p = 0.008) and rs6313 (p = 0.026) showed genetic association with schizophrenia. Differential DNA methylation was associated with rs6314 and rs6313. There was a strong correlation between HTR2A DNA methylation and mRNA expression. The results were nominally significant but did not survive the rigorous Benjamini-Hochberg correction for multiple testing. Differential HTR2A expression in schizophrenia in our study may be the result of the combined effect of multiple differentially methylated CpG sites. Epigenetic HTR2A regulation may alter brain function, which contributes to the development of schizophrenia.

  3. The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Wei [Chinese Academy of Sciences, 5 Datun Road, Chaoyang District, Beijing 100101 (China); University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049 (China); Gao, Feng [Chinese Academy of Sciences, 5 Datun Road, Chaoyang District, Beijing 100101 (China); Fan, Haitian; Shan, Xiaoyue [Chinese Academy of Sciences, 5 Datun Road, Chaoyang District, Beijing 100101 (China); University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049 (China); Sun, Renhua [Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing 100093 (China); University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049 (China); Liu, Lin, E-mail: liulin@ibcas.ac.cn [Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing 100093 (China); Gong, Weimin, E-mail: liulin@ibcas.ac.cn [Chinese Academy of Sciences, 5 Datun Road, Chaoyang District, Beijing 100101 (China)

    2013-05-01

    The crystal structures of Arabidopsis Deg5 and Deg8 have been determined to resolutions of 2.6 and 2.0 Å, respectively, revealing novel structural features of HtrA proteases. Plant Deg5 and Deg8 are two members of the HtrA proteases, a family of oligomeric serine endopeptidases that are involved in a variety of protein quality-control processes. These two HtrA proteases are located in the thylakoid lumen and participate in high-light stress responses by collaborating with other chloroplast proteins. Deg5 and Deg8 degrade photodamaged D1 protein of the photosystem II reaction centre, allowing its in situ replacement. Here, the crystal structures of Arabidopsis thaliana Deg5 (S266A) and Deg8 (S292A) are reported at 2.6 and 2.0 Å resolution, respectively. The Deg5 trimer contains two calcium ions in a central channel, suggesting a link between photodamage control and calcium ions in chloroplasts. Previous structures of HtrA proteases have indicated that their regulation usually requires C-terminal PDZ domain(s). Deg5 is unique in that it contains no PDZ domain and the trimeric structure of Deg5 (S266A) reveals a novel catalytic triad conformation. A similar triad conformation is observed in the hexameric structure of the single PDZ-domain-containing Deg8 (S292A). These findings suggest a novel activation mechanism for plant HtrA proteases and provide structural clues to their function in light-stress response.

  4. The roles of CC2D1A and HTR1A gene expressions in autism spectrum disorders.

    Science.gov (United States)

    Sener, Elif Funda; Cıkılı Uytun, Merve; Korkmaz Bayramov, Keziban; Zararsiz, Gokmen; Oztop, Didem Behice; Canatan, Halit; Ozkul, Yusuf

    2016-06-01

    Classical autism belongs to a group of heterogeneous disorders known as autism spectrum disorders (ASD). Autism is defined as a neurodevelopmental disorder, characterized by repetitive stereotypic behaviors or restricted interests, social withdrawal, and communication deficits. Numerous susceptibility genes and chromosomal abnormalities have been reported in association with autism but the etiology of this disorder is unknown in many cases. CC2D1A gene has been linked to mental retardation (MR) in a family with a large deletion before. Intellectual disability (ID) is a common feature of autistic cases. Therefore we aimed to investigate the expressions of CC2D1A and HTR1A genes with the diagnosis of autism in Turkey. Forty-four autistic patients (35 boys, 9 girls) and 27 controls were enrolled and obtained whole blood samples to isolate RNA samples from each participant. CC2D1A and HTR1A gene expressions were assessed by quantitative Real-Time PCR (qRT-PCR) in Genome and Stem Cell Center, Erciyes University. Both expressions of CC2D1A and HTR1A genes studied on ASD cases and controls were significantly different (p < 0.001). The expression of HTR1A was undetectable in the ASD samples. Comparison of ID and CC2D1A gene expression was also found statistically significant (p = 0.028). CC2D1A gene expression may be used as a candidate gene for ASD cases with ID. Further studies are needed to investigate the potential roles of these CC2D1A and HTR1A genes in their related pathways in ASD.

  5. Relationship between genetic polymorphisms in the HTR1A gene and paranoid schizophrenia in a northern Han Chinese population.

    Science.gov (United States)

    Zhou, Xue; Ding, Mei; Ding, Chunli; Yao, Jun; Pang, Hao; Xing, Jiaxin; Xuan, Jinfeng; Wang, Baojie

    2013-03-01

    The hypothesis for the etiology of schizophrenia involves various neurotransmitters, including 5-HT. Metabolic disorder of 5-HT is an important underlying neurobiochemical cause leading to the development of mental illness. Abnormality in the receptors involved in 5-HT synthesis and metabolism may affect the functioning of 5-HT in the central nervous system. There are seven types of 5-HT receptor families, with a total of 15 corresponding subtypes. HTR1A is the most abundantly expressed 5-HT receptor subtype in the mammalian brain. SNPs in HTR1A enhance or weaken the functioning of 5-HT by affecting HTR1A expression levels or ligand-binding activity, thereby placing HTR1A in an important role in the study of diseases of the nervous system. This study employed DNA sequencing to investigate HTR1A fragment lengths, including complete exons as well as 5' FR and 3' FR segments, for a total of 2,718 bp. Seven SNP loci (ss212928868, rs6295, rs6294, ss218178047, rs34118353, rs6449693, and rs878567) were found in 182 healthy volunteers and 161 patients. Among them, two SNP loci had not been reported in the National Center for Biotechnology Information (NCBI) database, promoter locus ss212928868 and exon locus ss218178047, which now have been approved by the NCBI database and assigned rs numbers, rs113195492 and rs112846276, respectively. ss212928868 and rs6294 were statistically different between control and paranoid schizophrenic women (P paranoid schizophrenic and control group women. Although such differences were lost after statistical correction, studies with larger sample sizes have not been conducted. Combined with the newly discovered loci, these findings can point out possible directions for future investigations in different populations.

  6. Thermohydraulic simulation of HTR-10 nuclear reactor core using realistic CFD approach; Simulacao termohidraulica do nucleo do reator nuclear HTR-10 com o uso da abordagem realistica CFD

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Alexandro S.; Dominguez, Dany S., E-mail: alexandrossilva@gmail.com, E-mail: dsdominguez@gmail.com [Universidade Estadual de Santa Cruz (UESC), Ilheus, BA (Brazil); Mazaira, Leorlen Y. Rojas; Hernandez, Carlos R.G., E-mail: leored1984@gmail.com, E-mail: cgh@instec.cu [Instituto Superior de Tecnologias y Ciencias Aplicadas, La Habana (Cuba); Lira, Carlos Alberto Brayner de Oliveira, E-mail: cabol@ufpe.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2015-07-01

    High-temperature gas-cooled reactors (HTGRs) have the potential to be used as possible energy generation sources in the near future, owing to their inherently safe performance by using a large amount of graphite, low power density design, and high conversion efficiency. However, safety is the most important issue for its commercialization in nuclear energy industry. It is very important for safety design and operation of an HTGR to investigate its thermal–hydraulic characteristics. In this article, it was performed the thermal–hydraulic simulation of compressible flow inside the core of the pebble bed reactor HTR (High Temperature Reactor)-10 using Computational Fluid Dynamics (CFD). The realistic approach was used, where every closely packed pebble is realistically modelled considering a graphite layer and sphere of fuel. Due to the high computational cost is impossible simulate the full core; therefore, the geometry used is a column of FCC (Face Centered Cubic) cells, with 41 layers and 82 pebbles. The input data used were taken from the thermohydraulic IAEA Benchmark (TECDOC-1694). The results show the profiles of velocity and temperature of the coolant in the core, and the temperature distribution inside the pebbles. The maximum temperatures in the pebbles do not exceed the allowable limit for this type of nuclear fuel. (author)

  7. Numerical discretization analysis of a HTR steam generator model for the thermal-hydraulics code trace

    Directory of Open Access Journals (Sweden)

    Esch Markus

    2014-01-01

    Full Text Available For future high temperature reactor projects, e. g., for electricity production or nuclear process heat applications, the steam generator is a crucial component. A typical design is a helical coil steam generator consisting of several tubes connected in parallel forming cylinders of different diameters. This type of steam generator was a significant component used at the thorium high temperature reactor. In the work presented the temperature profile is being analyzed by the nodal thermal hydraulics code TRACE for the thorium high temperature reactor steam generator. The influence of the nodalization is being investigated within the scope of this study and compared to experimental results from the past. The results of the standard TRACE code are compared to results using a modified Nusselt number for the primary side. The implemented heat transfer correlation was developed within the past German HTR program. This study shows that both TRACE versions are stable and provides a discussion of the nodalization requirements.

  8. Pebble bed reactors simulation using MCNP: The Chinese HTR-10 reactor

    Directory of Open Access Journals (Sweden)

    SA Hosseini

    2013-09-01

    Full Text Available   Given the role of Gas-Graphite reactors as the fourth generation reactors and their recently renewed importance, in 2002 the IAEA proposed a set of Benchmarking problems. In this work, we propose a model both efficient in time and resources and exact to simulate the HTR-10 reactor using MCNP-4C code. During the present work, all of the pressing factors in PBM reactor design such as the inter-pebble leakage, fuel particle distribution and fuel pebble packing fraction effects have been taken into account to obtain an exact and easy to run model. Finally, the comparison between the results of the present work and other calculations made at INEEL proves the exactness of the proposed model.

  9. Abrasion behavior of graphite pebble in lifting pipe of pebble-bed HTR

    Energy Technology Data Exchange (ETDEWEB)

    Shen, Ke; Su, Jiageng [Institute of Nuclear and New Energy Technology, Advanced Nuclear Energy Technology Cooperation Innovation Center, The Key Laboratory of Advanced Nuclear Engineering and Safety, Ministry of Education, Beijing 10084 (China); Zhou, Hongbo [Institute of Nuclear and New Energy Technology, Advanced Nuclear Energy Technology Cooperation Innovation Center, The Key Laboratory of Advanced Nuclear Engineering and Safety, Ministry of Education, Beijing 10084 (China); Chinergy Co., LTD., Beijing 100193 (China); Peng, Wei; Liu, Bing [Institute of Nuclear and New Energy Technology, Advanced Nuclear Energy Technology Cooperation Innovation Center, The Key Laboratory of Advanced Nuclear Engineering and Safety, Ministry of Education, Beijing 10084 (China); Yu, Suyun, E-mail: suyuan@tsinghua.edu.cn [Center for Combustion Energy, The Key Laboratory for Thermal Science and Power Engineering, Ministry of Educations, Tsinghua University, Beijing 10084 (China)

    2015-11-15

    Highlights: • Quantitative determination of abrasion rate of graphite pebbles in different lifting velocities. • Abrasion behavior of graphite pebble in helium, air and nitrogen. • In helium, intensive collisions caused by oscillatory motion result in more graphite dust production. - Abstract: A pebble-bed high-temperature gas-cooled reactor (pebble-bed HTR) uses a helium coolant, graphite core structure, and spherical fuel elements. The pebble-bed design enables on-line refueling, avoiding refueling shutdowns. During circulation process, the pebbles are lifted pneumatically via a stainless steel lifting pipe and reinserted into the reactor. Inevitably, the movement of the fuel elements as they recirculate in the reactor produces graphite dust. Mechanical wear is the primary source of graphite dust production. Specifically, the sources are mechanisms of pebble–pebble contact, pebble–wall (structural graphite) contact, and fuel handling (pebble–metal abrasion). The key contribution to graphite dust production is from the fuel handling system, particularly from the lifting pipe. During pneumatic lift, graphite pebbles undergo multiple collisions with the stainless steel lifting pipe, thereby causing abrasion of the graphite pebbles and producing graphite dust. The present work explored the abrasion behavior of graphite pebble in the lifting pipe by measuring the abrasion rate at different lifting velocities. The abrasion rate of the graphite pebble in helium was found much higher than those in air and nitrogen. This gas environment effect could be explained by either tribology behavior or dynamic behavior. Friction testing excluded the possibility of tribology reason. The dynamic behavior of the graphite pebble was captured by analysis of the audio waveforms during pneumatic lift. The analysis results revealed unique dynamic behavior of the graphite pebble in helium. Oscillation and consequently intensive collisions occur during pneumatic lift, causing

  10. Regulation of HtrA2 on WT1 gene expression under imatinib stimulation and its effects on the cell biology of K562 cells.

    Science.gov (United States)

    Zhang, Lixia; Li, Yan; Li, Xiaoyan; Zhang, Qing; Qiu, Shaowei; Zhang, Qi; Wang, Min; Xing, Haiyan; Rao, Qing; Tian, Zheng; Tang, Kejing; Wang, Jianxiang; Mi, Yingchang

    2017-09-01

    The aim of the present study was to investigate the regulation of Wilms Tumor 1 (WT1) by serine protease high-temperature requirement protein A2 (HtrA2), a member of the Htr family, in K562 cells. In addition, the study aimed to observe the effect of this regulation on cell biological functions and its associated mechanisms. Expression of WT1 and HtrA2 mRNA, and proteins following imatinib and the HtrA2 inhibitor 5-[5-(2-nitrophenyl) furfuryl iodine]-1, 3-diphenyl-2-thiobarbituric acid (UCF-101) treatment was detected with reverse transcription-quantitative polymerase chain reaction and western blot analysis. Subsequent to treatment with drugs and UCF-101, the proliferative function of K562 cells was detected using MTT assays, and the rate of apoptosis was detected using Annexin V with propidium iodide flow cytometry in K562 cells. The protein levels in the signaling pathway were analyzed using western blotting following treatment with imatinib and UCF-101. In K562 cells, imatinib treatment activated HtrA2 gene at a transcription level, while the WT1 gene was simultaneously downregulated. Following HtrA2 inhibitor (UCF-101) treatment, the downregulation of WT1 increased gradually. At the protein level, imatinib induced the increase in HtrA2 protein level and concomitantly downregulated WT1 protein level. Subsequent to HtrA2 inhibition by UCF-101, the WT1 protein level decreased temporarily, but eventually increased. Imatinib induced apoptosis in K562 cells, but this effect was attenuated by the HtrA2 inhibitor UCF-101, resulting in the upregulation of the WT1 protein level. However; UCF-101 did not markedly change the proliferation inhibition caused by imatinib. Imatinib activated the p38 mitogen activated protein kinase (p38 MAPK) signaling pathway in K562 cells, and UCF-101 affected the activation of imatinib in the p38 MAPK signaling pathway. Imatinib inhibited the extracellular signal-related kinase (ERK1/2) pathway markedly and persistently, but UCF-101

  11. Prediction of extracellular proteases of the human pathogen Helicobacter pylori reveals proteolytic activity of the Hp1018/19 protein HtrA.

    Directory of Open Access Journals (Sweden)

    Martin Löwer

    Full Text Available Exported proteases of Helicobacter pylori (H. pylori are potentially involved in pathogen-associated disorders leading to gastric inflammation and neoplasia. By comprehensive sequence screening of the H. pylori proteome for predicted secreted proteases, we retrieved several candidate genes. We detected caseinolytic activities of several such proteases, which are released independently from the H. pylori type IV secretion system encoded by the cag pathogenicity island (cagPAI. Among these, we found the predicted serine protease HtrA (Hp1019, which was previously identified in the bacterial secretome of H. pylori. Importantly, we further found that the H. pylori genes hp1018 and hp1019 represent a single gene likely coding for an exported protein. Here, we directly verified proteolytic activity of HtrA in vitro and identified the HtrA protease in zymograms by mass spectrometry. Overexpressed and purified HtrA exhibited pronounced proteolytic activity, which is inactivated after mutation of Ser205 to alanine in the predicted active center of HtrA. These data demonstrate that H. pylori secretes HtrA as an active protease, which might represent a novel candidate target for therapeutic intervention strategies.

  12. Next-Generation Bacillus anthracis Live Attenuated Spore Vaccine Based on the htrA(-) (High Temperature Requirement A) Sterne Strain.

    Science.gov (United States)

    Chitlaru, Theodor; Israeli, Ma'ayan; Bar-Haim, Erez; Elia, Uri; Rotem, Shahar; Ehrlich, Sharon; Cohen, Ofer; Shafferman, Avigdor

    2016-01-06

    Anthrax is a lethal disease caused by the gram-positive spore-producing bacterium Bacillus anthracis. Live attenuated vaccines, such as the nonencapsulated Sterne strain, do not meet the safety standards mandated for human use in the Western world and are approved for veterinary purposes only. Here we demonstrate that disrupting the htrA gene, encoding the chaperone/protease HtrA (High Temperature Requirement A), in the virulent Bacillus anthracis Vollum strain results in significant virulence attenuation in guinea pigs, rabbits and mice, underlying the universality of the attenuated phenotype associated with htrA knockout. Accordingly, htrA disruption was implemented for the development of a Sterne-derived safe live vaccine compatible with human use. The novel B. anthracis SterneΔhtrA strain secretes functional anthrax toxins but is 10-10(4)-fold less virulent than the Sterne vaccine strain depending on animal model (mice, guinea pigs, or rabbits). In spite of this attenuation, double or even single immunization with SterneΔhtrA spores elicits immune responses which target toxaemia and bacteremia resulting in protection from subcutaneous or respiratory lethal challenge with a virulent strain in guinea pigs and rabbits. The efficacy of the immune-protective response in guinea pigs was maintained for at least 50 weeks after a single immunization.

  13. Estimation of Graphite Dust Quantity and Size Distribution of Graphite Particle in HTR-10%HTR-10产生石墨粉尘量的估算及其尺寸分布

    Institute of Scientific and Technical Information of China (English)

    雒晓卫; 于溯源; 张振声; 何树延

    2005-01-01

    在10MW高温气冷堆(HTR-10)中,由于石墨构件的磨损会产生石墨粉尘,将影响反应堆的正常运行.石墨粉尘的产生主要来自堆芯、卸料管和装料管3个部位.本文在石墨磨损试验的基础上,保守地估算了HTR-10在正常运行工况下石墨粉尘的产生量(大约为2.74kg/年).通过质量加权平均,给出了石墨粉尘的体积、面积和直径分布函数.

  14. 利用Cre/loxP系统构建无标记的htrA基因缺失的变链菌突变株%Construction of Unmarked HtrA-Deficient Mutant of Streptococcus Mutans with Cre/loxP System

    Institute of Scientific and Technical Information of China (English)

    张文娟; 于丹妮; 韩育植; 任志明

    2011-01-01

    Objective: To construct the Streptococcus mutans (S. mutans) htrA-deficient mutant and to remove the antibiotic resistance marker with the Cre/loxP site-specific recombination system. Methods: The DNA fragment containing htrA was amplified by PCR and cloned into the pGEM-T-Easy TA cloning vector. Then, a kanamycin resistance cassette ( loxP-Km-loxP ) was inserted into this recomhinant plasmid and replaced a part of the htrA gene, yielding homologous recombination plasmid pIB △ htrA-Km. The electrotransformation of S. mutans cells with pIB △ htrA-Km resulted in the isolation of kanamycin resistant S. mutans transformant. One such mutant was transformed with a cre expression plasmid (pCrePA). The kanamycin resistance gene was then excised. The pCrePA was then easily eliminated at the nonpermissive temperature, resulting in a markerless mutant strain carrying a deletion at the htrA loci, which was verif'ied by PCR and DNA sequencing. Results: The deletion of' a part of htrA and the kanamycin resistance gene was confirmed by PCR analysis and DNA sequencing.There was a loxP at this locus. Conclusion: A htrA-deficient mutant of S. mutans was construrted and the antibiotic resistance marker was deleted successfully, which can help to further study the role of htrA in the pathogenesis of S.mutans.%目的:利用Cre/loxP位点特异性重组系统构建口腔变异链球菌htrA基因缺陷株,并删除选择标记.方法:PCR扩增变链菌的htrA基因片段并克隆到pGEM-T-Easy TA载体.随后插入卡那霉素(Km)抗性基因盒(loxP-Km-loxP)并替换htrA基因的部分序列,使htrA基因失活,构建出htrA基因缺失的同源重组载体pIB△htrA-Km.将该质粒电转化变链菌标准株,抗生素筛选出发生同源重组的菌株,再用含cre基因的热敏质粒pCrePA电转化,删除Km抗性基因.在限制性温度下培养,消除质粒pCrePA,获得无标记的变链菌htrA基因缺陷株,经PCR及DNA测序鉴定.结果:PCR及DNA测序分析证明htr

  15. Association of serotonin transporter (SLC6A4 & receptor (5HTR1A, 5HTR2A polymorphisms with response to treatment with escitalopram in patients with major depressive disorder : A preliminary study

    Directory of Open Access Journals (Sweden)

    Aniruddha Basu

    2015-01-01

    Full Text Available Background & objectives: Genetic factors have potential of predicting response to antidepressants in patients with major depressive disorder (MDD. In this study, an attempt was made to find an association between response to escitalopram in patients with MDD, and serotonin transporter (SLC6A4 and receptor (5HTR1A, 5HTR2A polymorphisms. Methods: Fifty five patients diagnosed as suffering from MDD, were selected for the study. The patients were treated with escitalopram over a period of 6-8 wk. Severity of depression, response to treatment and side effects were assessed using standardised instruments. Genetic variations from HTR1A (rs6295, HTR2A (rs6311 and rs6313 and SLC6A4 (44 base-pair insertion/deletion at 5-HTTLPR were genotyped. The genetic data of the responders and non-responders were compared to assess the role of genetic variants in therapeutic outcome. Results: Thirty six (65.5% patients responded to treatment, and 19 (34.5% had complete remission. No association was observed for genotype and allelic frequencies of single nucleotide polymorphisms (SNPs among remitter/non-remitter and responder/non-responder groups, and six most common side-effects, except memory loss which was significantly associated with rs6311 ( p0 =0.03. Interpretation & conclusions: No significant association was found between the SNPs analysed and response to escitalopram in patients with MDD though a significant association was seen between the side effect of memory loss and rs6311. Studies with larger sample are required to find out genetic basis of antidepressant response in Indian patients.

  16. Loss of HtrA2/Omi activity in non-neuronal tissues of adult mice causes premature aging.

    Science.gov (United States)

    Kang, S; Louboutin, J-P; Datta, P; Landel, C P; Martinez, D; Zervos, A S; Strayer, D S; Fernandes-Alnemri, T; Alnemri, E S

    2013-02-01

    mnd2 mice die prematurely as a result of neurodegeneration 30-40 days after birth due to loss of the enzymatic activity of the mitochondrial quality control protease HtrA2/Omi. Here, we show that transgenic expression of human HtrA2/Omi in the central nervous system of mnd2 mice rescues them from neurodegeneration and prevents their premature death. Interestingly, adult transgenic mnd2 mice develop accelerated aging phenotypes, such as premature weight loss, hair loss, reduced fertility, curvature of the spine, heart enlargement, increased autophagy, and death by 12-17 months of age. These mice also have elevated levels of clonally expanded mitochondrial DNA (mtDNA) deletions in their tissues. Our results provide direct genetic evidence linking mitochondrial protein quality control to mtDNA deletions and aging in mammals.

  17. Application of grey model on analyzing the passive natural circulation residual heat removal system of HTR-10

    Institute of Scientific and Technical Information of China (English)

    ZHOU Tao; PENG Changhong; WANG Zenghui; WANG Ruosu

    2008-01-01

    Using the grey correlation analysis, it can be concluded that the reactor pressure vessel wall temperature has the strongest effect on the passive residual heat removal system in HTR (High Temperature gas-cooled Reactor),the chimney height takes the second place, and the influence of inlet air temperature of the chimney is the least. This conclusion is the same as that analyzed by the traditional method. According to the grey model theory, the GM(1,1) and GM(1, 3) model are built based on the inlet air temperature of chimney, pressure vessel temperature and the chimney height. Then the effect of three factors on the heat removal power is studied in this paper. The model plays an important role on data prediction, and is a new method for studying the heat removal power. The method can provide a new theoretical analysis to the passive residual heat removal system of HTR.

  18. CVD 908, CVD 908-htrA, and CVD 909 live oral typhoid vaccines: a logical progression.

    Science.gov (United States)

    Tacket, Carol O; Levine, Myron M

    2007-07-15

    Typhoid fever remains an important public health problem in many parts of the world. Despite the availability of oral Ty21a (Vivotif; Berna Biotech) and parenteral Vi polysaccharide vaccine (Typhim Vi; Aventis Pasteur), improved typhoid fever vaccines have been sought. These include a series of vaccine candidates developed at the Center for Vaccine Development, University of Maryland, based on attenuation of Salmonella enterica serovar Typhi by deletions in the aroC, aroD, and htrA genes. These vaccine candidates, designated "CVD 908," "CVD 908-htrA," and "CVD 909," have been developed and tested in volunteers with variable success. This review summarizes the clinical data that directed the logical progression of this vaccine development strategy.

  19. Construction of a HtrA deficient Streptococcus mutans strain and its transformation ability%乳牙高毒力变异链球菌 HtrA 缺陷株的构建及转化力的研究

    Institute of Scientific and Technical Information of China (English)

    张宏柱; 黄萍; 刘兴容

    2015-01-01

    目的:构建乳牙高毒力变异链球菌 HtrA 基因缺陷株(简称 HtrA 缺陷株),比较 HtrA 缺陷株和高毒力株的转化力。方法:将乳牙变异链球菌 HtrA 高毒力菌株复苏,厌氧培养,应用基因同源重组技术进行 HtrA基因缺陷株的构建。以含有 HtrA 基因的变异链球菌标准株 DNA 作为参照进行 PCR 鉴定。将乳牙变异链球菌HtrA 高毒力株与 HtrA 缺陷菌株分别在 TPY 液体培养基中增菌,厌氧培养,以菌落计数来比较二者的转化力。结果:凝胶自动成像分析结果显示,HtrA 缺陷株在500 bp 未出现 HtrA 基因片段的特异性电泳条带,而在710、1200、1100 bp 处分别出现了红霉素抗性基因片段、HtrA 基因上游引物序列合并红霉素抗性基因引物 HtrAUF/P1以及 HtrA 基因下游引物序列合并红霉素抗性基因引物 HtrAUR/P2的特异性条带。而 HtrA 高毒力株的表现则相反。转化力检测结果显示:6、9、12、24、48 h,高毒力株的菌落数量均明显多于 HtrA 基因缺陷株(P <0.05)。结论:通过基因重组等方法可以将变异链球菌高毒力株中的 HtrA 基因敲除,构建高毒力变异链球菌 HtrA 基因缺陷株;变异链球菌高毒力株的转化力高于 HtrA 基因缺陷株,提示 HtrA 与变异链球菌的致龋性有关。%Abstract] AIM:To construct a HtrA deficient strain of Streptococcus mutans(S.mutans),and to compare the transformation ability of the deficient strain with HtrA high virulence strain.METHODS:S.mutans strain with HtrA high virulence was cultured in anaerobic condition and the competent cells were prepared.Recombinant plasmid vector as inserted into the cells and homologous recombination technique was used to construct the HtrA deficient strain of S.mutans.The constructed strain was identified by PCR method.The transformation ability of the 2 strains was in-vestigated by counting the number of colonies cultured on TPY

  20. Associations of the 5-hydroxytryptamine (serotonin) receptor 1B gene (HTR1B) with alcohol, cocaine, and heroin abuse.

    Science.gov (United States)

    Cao, Jian; LaRocque, Emily; Li, Dawei

    2013-03-01

    Abnormal serotonergic pathways are implicated in numerous neuropsychiatric disorders including alcohol and drug dependence (abuse). The human 5-hydroxytryptamine (serotonin) receptor 1B, encoded by the HTR1B (5-HT1B) gene, is a presynaptic serotonin autoreceptor that plays an important role in regulating serotonin synthesis and release. Although there was evidence of associations of the HTR1B gene variants in the etiologies of substance use disorders, negative findings were also reported. To clarify the roles of commonly reported single nucleotide polymorphisms (SNPs) of the HTR1B gene underlying alcohol and drug dependence (abuse), we performed a meta-analysis based on the available genotype data from individual candidate gene-based association studies. Evidence of association was found between the functional SNP -161A>T (rs130058) and alcohol, cocaine, and heroin dependence (e.g., P = 0.03 and odds ratio (OR) = 1.2 (1.02, 1.42) in the combined European, Asian, African, and Hispanic populations). SNP -261T>G (rs11568817) also showed evidence of association but with different directions in Europeans and non-Europeans (e.g., P = 0.0018 with OR = 1.42 (1.14, 1.76) and P = 0.01 with ORs = 0.5 (0.3, 0.85), respectively). This meta-analysis supports the associations of HTR1B -261T>G and -161A>T with alcohol and drug abuse and further investigations are warranted in larger samples.

  1. HtrC is involved in proteolysis of YpeB during germination of Bacillus anthracis and Bacillus subtilis spores.

    Science.gov (United States)

    Bernhards, Casey B; Chen, Yan; Toutkoushian, Hannah; Popham, David L

    2015-01-01

    Bacterial endospores can remain dormant for decades yet can respond to nutrients, germinate, and resume growth within minutes. An essential step in the germination process is degradation of the spore cortex peptidoglycan wall, and the SleB protein in Bacillus species plays a key role in this process. Stable incorporation of SleB into the spore requires the YpeB protein, and some evidence suggests that the two proteins interact within the dormant spore. Early during germination, YpeB is proteolytically processed to a stable fragment. In this work, the primary sites of YpeB cleavage were identified in Bacillus anthracis, and it was shown that the stable products are comprised of the C-terminal domain of YpeB. Modification of the predominant YpeB cleavage sites reduced proteolysis, but cleavage at other sites still resulted in loss of full-length YpeB. A B. anthracis strain lacking the HtrC protease did not generate the same stable YpeB products. In B. anthracis and Bacillus subtilis htrC mutants, YpeB was partially stabilized during germination but was still degraded at a reduced rate by other, unidentified proteases. Purified HtrC cleaved YpeB to a fragment similar to that observed in vivo, and this cleavage was stimulated by Mn(2+) or Ca(2+) ions. A lack of HtrC did not stabilize YpeB or SleB during spore formation in the absence of the partner protein, indicating other proteases are involved in their degradation during sporulation.

  2. Epigenetic and genetic variants in the HTR1B gene and clinical improvement in children and adolescents treated with fluoxetine.

    Science.gov (United States)

    Gassó, Patricia; Rodríguez, Natalia; Blázquez, Ana; Monteagudo, Ana; Boloc, Daniel; Plana, Maria Teresa; Lafuente, Amalia; Lázaro, Luisa; Arnaiz, Joan Albert; Mas, Sergi

    2017-04-03

    The serotonin 1B receptor (5-HT1B) is important to both the pathogenesis of major depressive disorder and the antidepressant effects of selective serotonin reuptake inhibitors. Although fluoxetine has been shown to be effective and safe in children and adolescents, not all patients experience a proper clinical response, which has led to further study into the main factors involved in this inter-individual variability. Our aim was to study the effect of epigenetic and genetic factors that could affect 5-hydroxytryptamine receptor 1B (HTR1B) gene expression, and thereby response to fluoxetine. A total of 83 children and adolescents were clinically assessed 12weeks after of initiating an antidepressant treatment with fluoxetine for the first time. We evaluated the influence of single nucleotide polymorphisms (SNPs) specifically located in transcription factor binding sites (TFBSs) on their clinical improvement. A combined genetic analysis considering the significant SNPs together with the functional variant rs130058 previously associated in our population was also performed. Moreover, we assessed, for the first time in the literature, whether methylation levels of the HTR1B promoter region could be associated with the pharmacological response. Two, rs9361233 and rs9361235, were significantly associated with clinical improvement after treatment with fluoxetine. The heterozygous genotype combination analysis showed a negative correlation with clinical improvement. The lowest improvement was experienced by patients who were heterozygous for all three SNPs. Moreover, a negative correlation was found between clinical improvement and the average methylation level of the HTR1B promoter. These results give new evidence for the role of epigenetic and genetic factors which could modulate HTR1B expression in the pharmacological response to antidepressants.

  3. Physical Analysis of the Initial Core and Running-In Phase for Pebble-Bed Reactor HTR-PM

    Directory of Open Access Journals (Sweden)

    Jingyu Zhang

    2017-01-01

    Full Text Available The pebble-bed reactor HTR-PM is being built in China and is planned to be critical in one or two years. At present, one emphasis of engineering design is to determine the fuel management scheme of the initial core and running-in phase. There are many possible schemes, and many factors need to be considered in the process of scheme evaluation and analysis. Based on the experience from the constructed or designed pebble-bed reactors, the fuel enrichment and the ratio of fuel spheres to graphite spheres are important. In this paper, some relevant physical considerations of the initial core and running-in phase of HTR-PM are given. Then a typical scheme of the initial core and running-in phase is proposed and simulated with VSOP code, and some key physical parameters, such as the maximum power per fuel sphere, the maximum fuel temperature, the refueling rate, and the discharge burnup, are calculated. Results of the physical parameters all satisfy the relevant design requirements, which means the proposed scheme is safe and reliable and can provide support for the fuel management of HTR-PM in the future.

  4. Dynamic Mathematical Model of Helical Coils of Steam Generator of HTR-10%HTR-10螺旋管式直流蒸汽发生器的动态数学模型

    Institute of Scientific and Technical Information of China (English)

    黄晓津; 冯元琨; 郭人俊

    2001-01-01

    为满足10MW高温气冷堆(HTR-10)控制系统分析设计的需要,分析了HTR-10螺旋管 式 直流蒸汽发生器内两相流动和传热的实际过程,根据建模的要求和可能获得的结构数据,选 择四方程漂移模型来描述螺旋管内两相流现象;经过仿真试算与实验总结,确定了模型中关 键的结构关系式和传热工况的判断逻辑,以此为基础,建立了可反映直流蒸汽发生器在正常 功率运行范围内动态特性的动态数学模型。通过对典型工况的动静态仿真计算和研究分析, 表明所得的结果与理论定性分析和实验吻合,从而证明了该模型的正确性。所建立的动态数 学模型还可以用于其他螺旋管式直流蒸汽发生器的研究设计。%In order to design and analyze control system of HTR-10, It is analyzed that the two-phase flow and heat transfer procedure inside the heli c al coils of one-through steam generator of HTR-10. Based on the requirement of m odel and structure property equations, the four-equation drift- fl ux model is selected to describe two-phase flow in the helical coils. The simul ating and exp erimenting determined the main structure property equations and judgement logica l of heat transfer situation. And the dynamic mathematical model that establishe d for steam generator of HTR-10 could describe steam generator’s dynamic chara c teristic at the normal running range of reactor.

  5. Effect of human mir-181a on function of trophblast cell line HTR-8/SVneo%mir-181a对滋养细胞HTR-8/SVneo功能的影响

    Institute of Scientific and Technical Information of China (English)

    延佳佳; 朱晓明; 尹国武; 任东青; 杨烨; 苗霞; 栾丽霞

    2013-01-01

    Objective MicroRNAs plays crucial roles in the process of pre-eclampsia(PE).We investigate the effect of mir-181a on apoptosis capacity of human trophblast cell line HTR-8/SVneo to identify the biological function of mir-181a in the process of pre-eclampsia.Methods The technology of transient transfection was used to enhance or suppress the expression of mir-181a in human trophblast cell line HTR-8/SVneo.The gene expression of mir-181a mRNA was assayed by reverse transcription-PCR.Then fluorescence microscope was used to study the effect on cell apoptosis.Results The reverse transcription-PCR results showed that the mir-181a gene expression between experiment group and control group (negative control group and blank control group)was obviously changed,with statistical significance(P <0.05).The apoptosis capacity of the HTR-8/SVneo between experiment group and control group was obviously changed,with statistical significance (P < 0.05).There was no statistical significance between negative control group and blank control group (P > 0.05).Conclusions mir-181a can promote the apoptosis capacity of the HTR-8/SVneo,so it could be the therapeutic target in the treatment of PE.%目的 MicroRNAs在子痫前期的发生发展过程中发挥了重要作用,本实验通过观察mir-181a对人滋养细胞HTR-8/SVneo凋亡功能的影响,进一步探讨mir-181a在子痫前期发病过程中的功能.方法 采用瞬时转染技术对人滋养细胞HTR-8/SVneo分别进行过表达与抑制mir-181a,应用RT-PCR检测mir-181a mRNA的表达.应用荧光显微镜检测细胞凋亡的变化.结果 RT-PCR显示过表达组和抑制组分别与阴性对照组和空白对照组相比均有明显差异(P<0.05),说明转染有效.细胞凋亡结果显示与阴性对照组和空白对照组相比,过表达mir-181a实验组细胞凋亡能力增加,抑制组细胞凋亡能力降低,差异有统计学意义(P<0.05);阴性对照组和空白对照组比较无统计学意义(P>0

  6. Thorium utilisation in a small long-life HTR. Part III: Composite-rod fuel blocks

    Energy Technology Data Exchange (ETDEWEB)

    Verrue, Jacques, E-mail: jacques.verrue@polytechnique.org [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB Delft (Netherlands); École Polytechnique (Member of ParisTech), 91128 Palaiseau Cedex (France); Ding, Ming, E-mail: dingm2005@gmail.com [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB Delft (Netherlands); Harbin Engineering University, Nantong Street 145, 150001 Harbin (China); Kloosterman, Jan Leen, E-mail: j.l.kloosterman@tudelft.nl [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB Delft (Netherlands)

    2014-02-15

    Highlights: • Composite-rod fuel blocks are proposed for a small block-type HTR. • An axial separation of fuel compacts is the most important feature. • Three patterns are presented to analyse the effects of the spatial distribution. • The spatial distribution has a large influence on the neutron spectrum. • Composite-rod fuel blocks reach a reactivity swing less than 4%. - Abstract: The U-Battery is a small long-life high temperature gas-cooled reactor (HTR) with power of 20 MWth. In order to increase its lifetime and diminish its reactivity swing, the concept of composite-rod fuel blocks with uranium and thorium was investigated. Composite-rod fuel blocks feature a specific axial separation between UO{sub 2} and ThO{sub 2} compacts in fuel rods. The design parameters, investigated by SCALE 6, include the number and spatial distribution of fuel compacts within the rods, the enrichment of uranium, the radii of fuel kernels and fuel compacts, and the packing fractions of uranium and thorium TRISO particles. The analysis shows that a lower moderation ratio and a larger inventory of heavy metals results in a lower reactivity swing. The optimal atomic carbon-to-heavy metal ratio depends on the mass fraction of U-235 and is commonly in the 160–200 range. The spatial distribution of the fuel compacts within the fuel rods has a large influence on the energy spectrum in each fuel compact and thus on the beginning-of-life reactivity and the reactivity swing. At end-of-life, the differences caused by the spatial distribution of the fuel compacts are smaller due to the fissions of U-233 in the ThO{sub 2} fuel compacts. This phenomenon enables to design fuel blocks with a very low reactivity swing, down to less than 4% in a 10-year lifetime. Among three types of thorium fuelled U-Battery blocks, the composite-rod fuel block achieves the highest end-of-life reactivity and the lowest reactivity swing.

  7. Fuel management of the HTR-10 including the equilibrium state and the running-in phase

    Energy Technology Data Exchange (ETDEWEB)

    Yang Yongwei E-mail: maoc@te.tsinghua.edu.cn; Luo Zhengpei; Jing Xingqing; Wu Zongxin

    2002-10-01

    The mode of fuel management of the HTR-10 was studied, including the simulation of the fuel shuffling process and the measurement of the burnup of a fuel element. The prior consideration was the design of the equilibrium state. Based on this the fuel loading of the initial core and the fuel shuffling mode from the initial core through the running-in phase into the equilibrium state were studied. The code system VSOP was used for the physical layout of the HTR-10 at the equilibrium state and in the running-in phase. For the equilibrium state, in order to lessen the difference between the peak and the average burnup, 5-fuel-passage-through-the-core was chosen for the fuel management. The average burnup of the spent fuel for the equilibrium core is 80000 MWd t{sup -1}, and the peak value of it is less than 100000 MWd t{sup -1} when the burnup of the recycled fuel element is under 72000 MWd t{sup -1}. The mixture of fuel element and graphite element was used for the initial core loading, the volume fractions of the fuel and the graphite elements were 0.57 and 0.43, respectively. During the running-in phase, the volume fraction of graphite will decrease with the fresh fuel elements being loaded from the top of the core and the graphite elements discharged from the bottom of the core. The fuel shuffling mode is similar to that of the equilibrium state. The burnup limit of recycled fuel element is also 72000 MWd t{sup -1} and the peak burnup is less than 100000 MWd t{sup -1}. Finally the core will be full of fuel elements with a certain profile of burnup and reaches the equilibrium state. According to the characteristics of the pebble-bed high temperature gas-cooled reactor, a calibrating method of concentration of {sup 137}Cs was proposed for the measurement of fuel burnup.

  8. Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived from a human trophoblast cell line HTR-8/SVneo.

    Science.gov (United States)

    Inagaki, Tetsunori; Kusunoki, Soshi; Tabu, Kouichi; Okabe, Hitomi; Yamada, Izumi; Taga, Tetsuya; Matsumoto, Akemi; Makino, Shintaro; Takeda, Satoru; Kato, Kiyoko

    2016-01-01

    The continual proliferation and differentiation of trophoblasts are critical for the maintenance of pregnancy. It is well known that the tissue stem cells are associated with the development of tissues and pathologies. It has been demonstrated that side-population (SP) cells identified by fluorescence-activated cell sorting (FACS) are enriched with stem cells. The SP cells in HTR-8/SVneo cells derived from human primary trophoblast cells were isolated by FACS. HTR-8/SVneo-SP cell cultures generated both SP and non-SP (NSP) subpopulations. In contrast, NSP cell cultures produced NSP cells and failed to produce SP cells. These SP cells showed self-renewal capability by serial colony-forming assay. Microarray expression analysis using a set of HTR-8/SVneo-SP and -NSP cells revealed that SP cells overexpressed several stemness genes including caudal type homeobox2 (CDX2) and bone morphogenic proteins (BMPs), and lymphocyte antigen 6 complex locus D (LY6D) gene was the most highly up-regulated in HTR-8/SVneo-SP cells. LY6D gene reduced its expression in the course of a 7-day cultivation in differentiation medium. SP cells tended to reduce its fraction by treatment of LY6D siRNA indicating that LY6D had potential to maintain cell proliferation of HTR-8/SVneo-SP cells. On ontology analysis, epithelial-mesenchymal transition (EMT) pathway was involved in the up-regulated genes on microarray analysis. HTR-SVneo-SP cells showed enhanced migration. This is the first report that LY6D was important for the maintenance of HTR-8/SVneo-SP cells. EMT was associated with the phenotype of these SP cells.

  9. Correlation Between Expression of High Temperature Requirement Serine Protease A1 (HtrA1) in Nucleus Pulposus and T2 Value of Magnetic Resonance Imaging.

    Science.gov (United States)

    Li, Dapeng; Yue, Jiawei; Jiang, Lu; Huang, Yonghui; Sun, Jifu; Wu, Yan

    2017-04-22

    BACKGROUND Degrading enzymes play an important role in the process of disc degeneration. The objective of this study was to investigate the correlation between the expression of high temperature requirement serine protease A1 (HtrA1) in the nucleus pulposus and the T2 value of the nucleus pulposus region in magnetic resonance imaging (MRI). MATERIAL AND METHODS Thirty-six patients who had undergone surgical excision of the nucleus pulposus were examined by MRI before surgery. Pfirrmann grading of the target intervertebral disc was performed according to the sagittal T2-weighted imaging, and the T2 value of the target nucleus pulposus was measured according to the median sagittal T2 mapping. The correlation between the Pfirrmann grade and the T2 value was analyzed. The expression of HtrA1 in the nucleus pulposus was analyzed by RT-PCR and Western blot. The correlation between the expression of HtrA1 and the T2 value was analyzed. RESULTS The T2 value of the nucleus pulposus region was 33.11-167.91 ms, with an average of 86.64±38.73 ms. According to Spearman correlation analysis, there was a rank correlation between T2 value and Pfirrmann grade (Pcorrelation coefficient (rs)=-0.93617. There was a linear correlation between the mRNA level of HtrA1 and T2 value in nucleus pulposus tissues (a=3.88, b=-0.019, F=112.63, Pcorrelation between the expression level of HtrA1 protein and the T2 value in the nucleus pulposus tissues (a=3.30, b=-0.016, F=93.15, P<0.0001) and normalized regression coefficient=-0.86. CONCLUSIONS The expression of HtrA1 was strongly related to the T2 value, suggesting that HtrA1 plays an important role in the pathological process of intervertebral disc degeneration.

  10. MALICE: un logiciel couplant la méthode intégrale et la méthode des éléments finis. Application à la fusion des oxydes en creuset froid

    Science.gov (United States)

    Servant, S.; Saumabere, D.; Gagnoud, A.; Du Terrail Couvat, Y.

    1992-11-01

    The multimethod Malice package allows to modelize coupled phenomena in the inducion elaboration. It is the result of the couping of 3D electromagnetic package OPHELIE and a generator of partial derivative equations FLUX EXPERT. The electromagnetic model is solved by integral method. It is well adapted to 3D problem, infinite domain of study and fine skin depth which is particularly intersting for the modelling of cold crucible installation. FLUX EXPERT uses finite element method to solve phenomenon equations: thermic and hydrodynamic phenomena are described in its equation base. In the particular application of fusion of oxyde in cold crucible, the results obtained enhance the interest of the package. The physical properties of these materials strongly depend on temperature, thus physical phenomena are coupled. Results calculated without coupling may lead to false exploitation of the process. Le logiciel MALICE modélise les phénomènes physiques couplés dans les installations d'élaboration par induction. Il est le résultat du couplage de deux logiciels: OPHELIE et FLUX-EXPERT. Le phénomène électromagnétique est résolu par la Méthode Intégrale (MI), bien adaptée au problème électromagnétique 3D notamment pour les domaines infinis et les régions où l'épaisseur de peau électromagnétique est faible. Ceci est particulièrement intéressant pour la modélisation du creuset froid. Le logiciel FLUX-EXPERT est basé sur la Méthode des Eléments Finis (MEF) et contient un générateur d'équations incluant la description des phénomènes thermique et hydrodynamique. Les résultats obtenus dans le cadre de la fusion des oxydes en creuset froid mettent en évidence l'intérêt du couplage. Les propriétés physiques des oxydes varient fortement avec la température et l'interaction entre les phénomènes est importante. Les résultats obtenus sans couplage sont erronés et peuvent conduire à un dimensionnement aberrant de l'installation de fusion.

  11. Omi/HtrA2对前列腺癌细胞株PED/PEA-15表达及PC-3细胞凋亡的影响%Effects of Omi/HtrA2 on Expression of Anti-apoptotic Protein PED/PEA-15 and Apoptosis of Prostate Cancer Cell Line PC-3

    Institute of Scientific and Technical Information of China (English)

    胡晓勇; 陈晓春; 朱朝辉; 陈朝晖; 曾甫清; 鲁功成

    2006-01-01

    背景与目的:促、抑凋亡因子间的相互作用与肿瘤的发生、发展密切相关.Omi/HtrA2是新近发现的一种凋亡调节因子,PED/PEA-15是一种广泛表达的抗凋亡蛋白.本研究旨在探讨Omi/HtrA2对PED/PEA-15表达和前列腺癌细胞PC-3凋亡的影响.方法:构建Omi/HtrA2的表达载体和siRNA载体,并用脂质体法分别将两载体转染至PC-3细胞中,Western blot和ELISA法检测Omi/HtrA2对PED/PEA-15表达和细胞凋亡的影响;Caspase-8检测试剂盒检测PED/PEA-15对Caspase-8活性的影响;Western blot、RT-PCR法检测Omi/HtrA2特异siRNA序列对其转录、翻译的影响,流式细胞仪检测siRNA导致Omi/HtrA2基因沉默后PC-3细胞凋亡的变化.结果:酶切和DNA测序证实Omi/HtrA2的表达载体和siRNA载体构建成功.通过转染Omi/HtrA2表达载体高表达Omi/HtrA2可抑制PED/PEA-1 5表达,并增加肿瘤细胞的凋亡率;抑制PED/PEA-15的表达可提高Caspase-8活性.siRNA沉默Omi/HtrA2基因后PC-3细胞对顺铂的敏感性降低.结论:Omi/HtrA2可通过抑制抗凋亡蛋白PED/PEA-15表达而在PC-3细胞凋亡中发挥重要作用.

  12. Modelling of HTR Confinement Behaviour during Accidents Involving Breach of the Helium Pressure Boundary

    Directory of Open Access Journals (Sweden)

    Joan Fontanet

    2009-01-01

    Full Text Available Development of HTRs requires the performance of a thorough safety study, which includes accident analyses. Confinement building performance is a key element of the system since the behaviour of aerosol and attached fission products within the building is of an utmost relevance in terms of the potential source term to the environment. This paper explores the available simulation capabilities (ASTEC and CONTAIN codes and illustrates the performance of a postulated HTR vented confinement under prototypical accident conditions by a scoping study based on two accident sequences characterized by Helium Pressure Boundary breaches, a small and a large break. The results obtained indicate that both codes predict very similar thermal-hydraulic responses of the confinement both in magnitude and timing. As for the aerosol behaviour, both codes predict that most of the inventory coming into the confinement is eventually depleted on the walls and only about 1% of the aerosol dust is released to the environment. The crosscomparison of codes states that largest differences are in the intercompartmental flows and the in-compartment gas composition.

  13. Nuclear graphite wear properties and estimation of graphite dust production in HTR-10

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Xiaowei, E-mail: xwluo@tsinghua.edu.cn; Wang, Xiaoxin; Shi, Li; Yu, Xiaoyu; Yu, Suyuan

    2017-04-15

    Highlights: • Graphite dust. • The wear properties of graphite. • Pebble bed. • High Temperature Gas-cooled Reactor. • Fuel element. - Abstract: The issue of the graphite dust has been a research focus for the safety of High Temperature Gas-cooled Reactors (HTGRs), especially for the pebble bed reactors. Most of the graphite dust is produced from the wear of fuel elements during cycling of fuel elements. However, due to the complexity of the motion of the fuel elements in the pebble bed, there is no systematic method developed to predict the amount the graphite dust in a pebble bed reactor. In this paper, the study of the flow of the fuel elements in the pebble bed was carried out. Both theoretical calculation and numerical analysis by Discrete Element Method (DEM) software PFC3D were conducted to obtain the normal forces and sliding distances of the fuel elements in pebble bed. The wearing theory was then integrated with PFC3D to estimate the amount of the graphite dust in a pebble bed reactor, 10 MW High Temperature gas-cooled test Reactor (HTR-10).

  14. Dynamic Modeling and Control Characteristics of the Two-Modular HTR-PM Nuclear Plant

    Directory of Open Access Journals (Sweden)

    Zhe Dong

    2017-01-01

    Full Text Available The modular high temperature gas-cooled reactor (MHTGR is a typical small modular reactor (SMR with inherent safety feature. Due to its high reactor outlet coolant temperature, the MHTGR can be applied not only for electricity production but also as a heat source for industrial complexes. Through multimodular scheme, that is, the superheated steam flows produced by multiple MHTGR-based nuclear supplying system (NSSS modules combined together to drive a common thermal load, the inherent safety feature of MHTGR is applicable to large-scale nuclear plants at any desired power ratings. Since the plant power control technique of traditional single-modular nuclear plants cannot be directly applied to the multimodular plants, it is necessary to develop the power control method of multimodular plants, where dynamical modeling, control design, and performance verification are three main aspects of developing plant control method. In this paper, the study in the power control for two-modular HTR-PM plant is summarized, and the verification results based on numerical simulation are given. The simulation results in the cases of plant power step and ramp show that the plant control characteristics are satisfactory.

  15. Recent advances on thermohydraulic simulation of HTR-10 nuclear reactor core using realistic CFD approach

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Alexandro S., E-mail: alexandrossilva@ifba.edu.br [Instituto Federal de Educacao, Ciencia e Tecnologia da Bahia (IFBA), Vitoria da Conquista, BA (Brazil); Mazaira, Leorlen Y.R., E-mail: leored1984@gmail.com, E-mail: cgh@instec.cu [Instituto Superior de Tecnologias y Ciencias Aplicadas (INSTEC), La Habana (Cuba); Dominguez, Dany S.; Hernandez, Carlos R.G., E-mail: alexandrossilva@gmail.com, E-mail: dsdominguez@gmail.com [Universidade Estadual de Santa Cruz (UESC), Ilheus, BA (Brazil). Programa de Pos-Graduacao em Modelagem Computacional; Lira, Carlos A.B.O., E-mail: cabol@ufpe.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2015-07-01

    High-temperature gas-cooled reactors (HTGRs) have the potential to be used as possible energy generation sources in the near future, owing to their inherently safe performance by using a large amount of graphite, low power density design, and high conversion efficiency. However, safety is the most important issue for its commercialization in nuclear energy industry. It is very important for safety design and operation of an HTGR to investigate its thermal-hydraulic characteristics. In this article, it was performed the thermal-hydraulic simulation of compressible flow inside the core of the pebble bed reactor HTR (High Temperature Reactor)-10 using Computational Fluid Dynamics (CFD). The realistic approach was used, where every closely packed pebble is realistically modelled considering a graphite layer and sphere of fuel. Due to the high computational cost is impossible simulate the full core; therefore, the geometry used is a FCC (Face Centered Cubic) cell with the half height of the core, with 21 layers and 95 pebbles. The input data used were taken from the thermal-hydraulic IAEA Bechmark. The results show the profiles of velocity and temperature of the coolant in the core, and the temperature distribution inside the pebbles. The maximum temperatures in the pebbles do not exceed the allowable limit for this type of nuclear fuel. (author)

  16. High Temperature Reactor (HTR) Deep Burn Core and Fuel Analysis: Design Selection for the Prismatic Block Reactor With Results from FY-2011 Activities

    Energy Technology Data Exchange (ETDEWEB)

    Michael A. Pope

    2011-10-01

    The Deep Burn (DB) Project is a U.S. Department of Energy sponsored feasibility study of Transuranic Management using high burnup fuel in the high temperature helium cooled reactor (HTR). The DB Project consists of seven tasks: project management, core and fuel analysis, spent fuel management, fuel cycle integration, TRU fuel modeling, TRU fuel qualification, and HTR fuel recycle. In the Phase II of the Project, we conducted nuclear analysis of TRU destruction/utilization in the HTR prismatic block design (Task 2.1), deep burn fuel/TRISO microanalysis (Task 2.3), and synergy with fast reactors (Task 4.2). The Task 2.1 covers the core physics design, thermo-hydraulic CFD analysis, and the thermofluid and safety analysis (low pressure conduction cooling, LPCC) of the HTR prismatic block design. The Task 2.3 covers the analysis of the structural behavior of TRISO fuel containing TRU at very high burnup level, i.e. exceeding 50% of FIMA. The Task 4.2 includes the self-cleaning HTR based on recycle of HTR-generated TRU in the same HTR. Chapter IV contains the design and analysis results of the 600MWth DB-HTR core physics with the cycle length, the average discharged burnup, heavy metal and plutonium consumptions, radial and axial power distributions, temperature reactivity coefficients. Also, it contains the analysis results of the 450MWth DB-HTR core physics and the analysis of the decay heat of a TRU loaded DB-HTR core. The evaluation of the hot spot fuel temperature of the fuel block in the DB-HTR (Deep-Burn High Temperature Reactor) core under full operating power conditions are described in Chapter V. The investigated designs are the 600MWth and 460MWth DB-HTRs. In Chapter VI, the thermo-fluid and safety of the 600MWth DB-HTRs has been analyzed to investigate a thermal-fluid design performance at the steady state and a passive safety performance during an LPCC event. Chapter VII describes the analysis results of the TRISO fuel microanalysis of the 600MWth and 450

  17. Identification of an RNA element for specific coordination of A-to-I RNA editing on HTR2C pre-mRNA.

    Science.gov (United States)

    Fukuda, Masatora; Oyama, Yui; Nishitarumizu, Azusa; Omura, Miki; Nose, Kanako; Deshimaru, Masanobu

    2015-10-01

    Adenosine-to-Inosine (A-to-I) RNA editing is an intracellular mechanism in which inosine is specifically substituted against adenosine by the action of adenosine deaminases acting on RNA (ADARs). Serotonin 2C receptor (HTR2C) is encoded through combinatorial A-to-I RNA editing at recoding sites (A - E site) on its pre-mRNA. Although the efficiency of RNA editing at particular sites is known to be critical for modulating the serotonin signaling, the mechanistic details of site-specific editing on HTR2C pre-mRNA are not fully understood. Toward complete understanding of this mechanism, we discovered an RNA element, which coordinates site-specific RNA editing on HTR2C pre-mRNA by an in vitro editing assay and secondary structural analysis of mutant HTR2C RNA fragments. Our results showed that HTR2C pre-mRNA forms a characteristic structure, which was restricted by the internal loop and Watson-Crick base-pair interaction on site E, for intrinsic editing. We suggest that the internal loop would contribute toward adjusting the relative distance and/or geometry between the editing sites and the scaffold for ADAR.

  18. Prediction of psychosis onset in Alzheimer disease: the role of depression symptom severity and the HTR2A T102C polymorphism.

    Science.gov (United States)

    Wilkosz, Patricia A; Kodavali, Chowdari; Weamer, Elise A; Miyahara, Sachiko; Lopez, Oscar L; Nimgaonkar, Vishwajit L; DeKosky, Steven T; Sweet, Robert A

    2007-12-05

    Psychotic symptoms in Alzheimer disease (AD + P) identify a heritable phenotype associated with a more severe course. We recently found an association of AD + P with depression symptom severity. Reports have shown an association of a serotonin-2A receptor (HTR2A) gene T102C polymorphism with AD + P and with depression during AD. We examined the interaction of this common genetic polymorphism with depression and increased psychosis risk. Subjects with possible or probable AD or mild cognitive impairment (MCI) without psychosis at study entry were genotyped for the HTR2A T102C polymorphism and reassessed every 6 months until psychosis onset. Psychotic and depressive symptoms were rated using the CERAD behavioral rating scale (CBRS). Cox proportional hazard models with time-dependent covariates were used to examine associations with psychosis onset. A total of 324 Caucasian subjects completed at least one follow-up exam. Depressive symptom severity was a strong predictor of psychosis onset. Neither psychosis onset nor depression severity was associated with the HTR2A genotype. Genotype interacted with depression severity to moderate the risk of AD + P onset. This did not result from an interaction of HTR2A genotype with antidepressant use. Psychosis onset in AD is strongly associated with severity of depressive symptoms, an association that may be modified by HTR2A genotype.

  19. Réalisation d'un réseau de pièges annulaires : une première étape vers une porte logique quantique avec des atomes froids

    Science.gov (United States)

    Dépret, B.; Verkerk, P.; Hennequin, D.

    2002-06-01

    L'objectif de ce projet est de réaliser une porte logique quantique basée sur l'interaction d'atomes dans deux sites voisins dans un réseau optique très désaccordé. Le principal obstacle rencontré vient du faible taux d'occupation des sites de tels réseaux. Nous proposons ici de mettre en oeuvre un nouveau type de réseaux optiques qui devraient pallier cette limitation. Il s'agit d'un réseau unidimensionnel de pièges annulaires “empilés” avec une période de λ/2. L'utilisation d'axicons conduit à une géométrie adaptée des faisceaux et donne le confinement radial dans les anneaux. Le mouvement azimutal peut également être limité par une force auxiliaire. Le bon taux d'occupation est lié au faible nombre de sites remplis à partir du nuage d'atomes froids initial.

  20. Htr2a gene and 5-HT2A receptor expression in the cerebral cortex studied using genetically modified mice

    Directory of Open Access Journals (Sweden)

    Rodrigo Andrade

    2010-08-01

    Full Text Available Serotonin receptors of the 5-HT2A subtype are robustly expressed in the cerebral cortex where they have been implicated in the pathophysiology and therapeutics of mental disorders and the actions of hallucinogens. Much less is known, however, about the specific cell types expressing 5-HT2A receptors in cortex. In the current study we use immunohistochemical and electrophysiological approaches in genetically modified mice to address the expression of the Htr2a gene and 5-HT2A receptors in cortex. We first use an EGFP expressing BAC transgenic mice and identify three main Htr2A gene expressing neuronal populations in cortex. The largest of these cell populations corresponds to layer V pyramidal cells of the anterior cortex, followed by GABAergic interneurons of the middle layers, and nonpyramidal cells of the subplate/Layer VIb. We then use 5-HT2A receptor knockout mice to identify an antibody capable of localizing 5-HT2A receptors in brain and use it to map these receptors. We find strong laminar expression of 5-HT2A receptors in cortex, especially along a diffuse band overlaying layer Va. This band exhibits a strong anteroposterior gradient that closely matches the localization of Htr2A expressing pyramidal cells of layer V. Finally we use electrophysiological and immunohistochemical approaches to show that most, but not all, GABAergic interneurons of the middle layers are parvalbumin expressing Fast-spiking interneurons and that these cells are depolarized and excited by serotonin, most likely through the activation of 5-HT2A receptors. These results clarify and extend our understanding of the cellular distribution of 5-HT2A receptors in the cerebral cortex.

  1. Rapid paracellular transmigration of Campylobacter jejuni across polarized epithelial cells without affecting TER: role of proteolytic-active HtrA cleaving E-cadherin but not fibronectin

    LENUS (Irish Health Repository)

    Boehm, Manja

    2012-04-25

    AbstractBackgroundCampylobacter jejuni is one of the most important bacterial pathogens causing food-borne illness worldwide. Crossing the intestinal epithelial barrier and host cell entry by C. jejuni is considered the primary reason of damage to the intestinal tissue, but the molecular mechanisms as well as major bacterial and host cell factors involved in this process are still widely unclear.ResultsIn the present study, we characterized the serine protease HtrA (high-temperature requirement A) of C. jejuni as a secreted virulence factor with important proteolytic functions. Infection studies and in vitro cleavage assays showed that C. jejuni’s HtrA triggers shedding of the extracellular E-cadherin NTF domain (90 kDa) of non-polarised INT-407 and polarized MKN-28 epithelial cells, but fibronectin was not cleaved as seen for H. pylori’s HtrA. Deletion of the htrA gene in C. jejuni or expression of a protease-deficient S197A point mutant did not lead to loss of flagella or reduced bacterial motility, but led to severe defects in E-cadherin cleavage and transmigration of the bacteria across polarized MKN-28 cell layers. Unlike other highly invasive pathogens, transmigration across polarized cells by wild-type C. jejuni is highly efficient and is achieved within a few minutes of infection. Interestingly, E-cadherin cleavage by C. jejuni occurs in a limited fashion and transmigration required the intact flagella as well as HtrA protease activity, but does not reduce transepithelial electrical resistance (TER) as seen with Salmonella, Shigella, Listeria or Neisseria.ConclusionThese results suggest that HtrA-mediated E-cadherin cleavage is involved in rapid crossing of the epithelial barrier by C. jejuni via a very specific mechanism using the paracellular route to reach basolateral surfaces, but does not cleave the fibronectin receptor which is necessary for cell entry.

  2. Rapid characterization of the binding property of HtrA2/Omi PDZ domain by validation screening of PDZ ligand library

    Institute of Scientific and Technical Information of China (English)

    SONG; ELi

    2007-01-01

    HtrA2/Omi is a mammalian mitochondrial serine protease, and was found to have dual roles in mam- malian cells, not only acting as an apoptosis-inducing protein but also maintaining mitochondrial ho- meostasis. PDZ domain is one of the most important protein-protein interaction modules and is in- volved in a variety of important cellular functions, such as signal transduction, degradation of proteins, and formation of cytoskeleton. Recently, it was reported that the PDZ domain of HtrA2/Omi might regulate proteolytic activity through its interactions with ligand proteins. In this study, we rapidly characterized the binding properties of HtrA2/Omi PDZ domain by validation screening of the PDZ ligand library with yeast two-hybrid approach. Then, we predicted its novel ligand proteins in human proteome and reconfirmed them in the yeast two-hybrid system. Finally, we analyzed the smallest networks bordered by the shortest path length between the protein pairs of novel interactions to evaluate the confidence of the identified interactions. The results revealed some novel binding proper- ties of HtrA2/Omi PDZ domain. Besides the reported Class II PDZ motif, it also binds to Class I and Class III motifs, and exhibits restricted variability at P?3, which means that the P?3 residue is selected according to the composition of the last three residues. Seven novel ligand proteins of HtrA2/Omi PDZ domain were discovered, providing significant clues for further clarifying the roles of HtrA2/Omi. Moreover, this study proves the high efficiency and practicability of the newly developed validation screening of candidate ligand library method for binding property characterization of peptide-binding domains.

  3. Rapid characterization of the binding property of HtrA2/Omi PDZ domain by validation screening of PDZ ligand library

    Institute of Scientific and Technical Information of China (English)

    MA SuCan; SONG ELi; GAO ShiJuan; TIAN Rui; GAO YouHe

    2007-01-01

    HtrA2/Omi is a mammalian mitochondrial serine protease, and was found to have dual roles in mammalian cells, not only acting as an apoptosis-inducing protein but also maintaining mitochondrial homeostasis. PDZ domain is one of the most important protein-protein interaction modules and is involved in a variety of important cellular functions, such as signal transduction, degradation of proteins,and formation of cytoskeleton. Recently, it was reported that the PDZ domain of HtrA2/Omi might regulate proteolytic activity through its interactions with ligand proteins. In this study, we rapidly characterized the binding properties of HtrA2/Omi PDZ domain by validation screening of the PDZ ligand library with yeast two-hybrid approach. Then, we predicted its novel ligand proteins in human proteome and reconfirmed them in the yeast two-hybrid system. Finally, we analyzed the smallest networks bordered by the shortest path length between the protein pairs of novel interactions to evaluate the confidence of the identified interactions. The results revealed some novel binding properties of HtrA2/Omi PDZ domain. Besides the reported Class Ⅱ PDZ motif, it also binds to Class Ⅰ and Class Ⅲ motifs, and exhibits restricted variability at P-3, which means that the P-3 residue is selected according to the composition of the last three residues. Seven novel ligand proteins of HtrA2/Omi PDZ domain were discovered, providing significant clues for further clarifying the roles of HtrA2/Omi.Moreover, this study proves the high efficiency and practicability of the newly developed validation screening of candidate ligand library method for binding property characterization of peptide-binding domains.

  4. Serine protease Omi/HtrA2 targets WARTS kinase to control cell proliferation.

    Science.gov (United States)

    Kuninaka, S; Iida, S-I; Hara, T; Nomura, M; Naoe, H; Morisaki, T; Nitta, M; Arima, Y; Mimori, T; Yonehara, S; Saya, H

    2007-04-12

    The serine protease Omi/HtrA2 was initially regarded as a proapoptotic molecule that proteolyses several proteins to induce cell death. Recent studies, however, indicate that loss of Omi protease activity increases susceptibility to stress-induced cell death. These complicated findings suggest that the protease activity of Omi is involved not only in apoptosis but also in cellular homeostasis. However, the targets which Omi uses to mediate this novel process are unknown. Previously, we showed that WARTS (WTS)/large tumor-suppressor 1 mitotic kinase interacts with the protein/discs-large protein/zonula (PDZ) domain of Omi and promotes its protease activity. We now report that WTS is a substrate for Omi protease activity, thus it is not only a regulator but also a downstream target of this protease. Interaction with Omi PDZ domain is required for WTS to be proteolysed. When caspase-9-deficient mouse embryonic fibroblasts (MEFs) were treated with staurosporine, WTS was proteolysed by activated endogenous Omi without induction of cell death. Therefore, protease activity of Omi and proteolysis of WTS are not necessarily required for cell death. We found that depletion of Omi from HeLa cells results in accelerated cell proliferation despite no significant change in the duration of mitosis. The depletion of WTS showed the same effect on S phase progression. Therefore, WTS proteolytic fragment(s) generated by Omi may act as an inhibitor of G1/S progression. Our data reveal a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase, suggesting a novel function of Omi other than apoptosis.

  5. Transient tests on blower trip and rod removal at the HTR-10

    Energy Technology Data Exchange (ETDEWEB)

    Hu Shouyin [Institute of Nuclear Energy Technology, Tsinghua University, P.O. Box 1021, Beijing 102201 (China)]. E-mail: hyyhtr@tsinghua.edu.cn; Wang Ruipian [Institute of Nuclear Energy Technology, Tsinghua University, P.O. Box 1021, Beijing 102201 (China); Gao Zuying [Institute of Nuclear Energy Technology, Tsinghua University, P.O. Box 1021, Beijing 102201 (China)

    2006-03-15

    Safety demonstration tests on the 10 MW high temperature gas-cooled reactor test module (HTR-10) were conducted to verify the inherent safety features of MHTGRs and to obtain the core and primary cooling system transient data for validation of safety analysis codes. Two simulated anticipated transients without scram (ATWS) tests, lose of forced cooling by trip of the helium blower and reactivity insertion via control rod withdrawal were performed. This paper describes the tests with detailed test method, condition and results. Calculated results show that the strongly negative temperature coefficient causes reactor power to closely follow heat removal levels. Maximum fuel temperature changes are limited by the large core heat capacity to below 1230 deg. C during two tests. The test of tripping the helium circulator ATWS test was conducted on October 15, 2003. Although none of 10 control rods was moved, the reactor power immediately decreased due to the negative temperature coefficient. After about 50 min, the reactor became criticality again. Finally, the reactor power went to a stable level with about 200 kW. The test of reactivity insertion ATWS test was conducted two times. Following the control rod withdrawal, the reactor power increased rapidly, the maximum power level reached to 5037 and 7230 kW from the initial power of 3000 kW in accordance with reactivity insertion of 0.136 and 0.689, respectively. After the reactivity introduced was compensated by means of the strong negative reactivity feedback effect, the reactor went to subcritical and the power decreased.

  6. Numerical Simulation of Single Droplet Evaporation in Steam Generator Annular Channel of HTR%HTR 蒸汽发生器环形通道内单液滴蒸发行为研究

    Institute of Scientific and Technical Information of China (English)

    何亮; 徐伟; 郑艳华

    2014-01-01

    高温气冷堆进水事故的机理研究对该反应堆安全具有重要意义。本文对液滴在蒸汽发生器环形通道内的流动和传热进行数值模拟研究,采用单液滴模型,利用龙格库塔方法进行求解。结果表明,初始直径小于420μm的液滴将无法运动到环形通道顶部。氦气压力的改变对于液滴完全蒸发需要的时间和液滴最大运动距离影响很小。%The mechanism research of water ingress accident in high‐temperature gas‐cooled reactor (HTR) has a significant meaning for the safety of HTR .The flow and heat transfer of droplet in the steam generator annular channel w ere simulated by using single droplet model and solved by Runge‐Kutta method in this paper .The results show that the droplet cannot reach at the top of the annular channel if the droplet’s initial diameter is less than 420 μm .The change of helium pressure has little effect on the droplet’s evaporation time and maximum movement distance .

  7. 小干扰RNA介导丝氨酸蛋白酶HtrA1沉默对光损伤人视网膜色素上皮细胞的影响%Influence of down-regulation of HtrA1 expression by small interfering RNA on light-injured human retinal pigment epithelium cells

    Institute of Scientific and Technical Information of China (English)

    余天; 邢怡桥; 陈长征

    2016-01-01

    Objective To observe the influence of down-regulation of HtrA1 expression by small interfering RNA on light-injured human retinal pigment epithelium (RPE) cells.Methods Cultured human RPE cells(8th-12th generations)were exposed to the blue light at the intensity of (2000 ± 500) Lux for 6 hours to establish the light injured model.Light injured cells were divided into HtrA1 siRNA group,negative control group and blank control group.HtrA1 siRNA group and negative control group were transfected with HtrA1 siRNA and control siRNA respectively.The proliferation of cells was assayed by CCK-8 method.Transwell test was used to detect the invasion ability of these three groups.Flow cytometry was used to detect the cell cycle and apoptosis.The expression of HtrA1 and vascular endothelial growth factor (VEGF)-A was detected by real time-polymerase chain reaction and Western blot respectively.Results The mRNA and protein level of HtrA1 in the light injured cells increased significantly compared to that in normal RPE cells (t=17.62,15.09;P<0.05).Compared with negative control group and blank control group,the knockdown of HtrA1 in HtrA1 siRNA group was associated with reduced cellular proliferation (t=6.37,4.52),migration (t =9.56,12.13),apoptosis (t =23.37,29.08) and decreased mRNA (t=17.36,11.32,7.29,4.05) and protein levels (t=12.02,15.28,4.98,6.24) of HtrA1 and VEGF-A.Cells of HtrA1 siRNA group mainly remained in G0/G1 phase,the difference was statistically significant (t=6.24,4.93;P <0.05).Conclusion Knockdown of HtrA1 gene may reduce the proliferation,migration capability and apoptosis of light-injured RPE cells,and decrease the expression of VEGF-A.%目的 观察丝氨酸蛋白酶HtrA1沉默对光损伤人视网膜色素上皮(RPE)细胞的影响.方法 体外培养人RPE细胞,取第8~12代生长良好的传代细胞用于实验.将细胞分为正常对照组、光损伤模型组.采用波长400 nm的蓝色节能灯以(2000±500) Lux持续光照RPE细胞6h建立

  8. Quantitative similarity analysis of the fluoride salt-cooled pebble bed reactor with HTR-10 and MSRE%氟盐冷却球床堆与HTR-10和MSRE的定量相似性分析

    Institute of Scientific and Technical Information of China (English)

    杨群; 于世和; 邹杨; 周波; 严睿; 徐洪杰

    2016-01-01

    Background: The fluoride salt-cooled pebble bed reactor which has not yet been built so far is a new international research reactor, so the selection of similar reactors with operation experience as benchmark is one important part of the applicability analysis for its reactor core nuclear design software. Purpose:This study aims to determine quantitatively the rationality of the 10 MW high-temperature gas-cooled test reactor (HTR-10) and the Molten Salt Reactor Experiment (MSRE) to be the benchmarks for the fluoride salt-cooled pebble bed reactor. Methods:The similarities of the fluoride salt-cooled pebble bed reactor with HTR-10 and MSRE were analyzed with the adoption of the similarity analysis software that is often used. Results: The results show that the fluoride salt-cooled pebble bed reactor and MSRE have a high similarity because they have the close spectrum peak energies and almost the same elements in core. The fluoride salt-cooled pebble bed reactor and HTR-10 have the different coolant and more differences in spectrum peak energies, hence the low similarity. Conclusion: MSRE is an ideal benchmark of the reactor core nuclear design software for the fluoride salt-cooled pebble bed reactor.%氟盐冷却球床堆是当前国际上一种新的研究堆型,尚无已经建造完成的反应堆,因此,选择相似且具有运行经验的反应堆作为基准题有助于堆芯核设计软件适用性分析。利用国际上常采用的相似性分析软件,可对熔盐实验堆(Molten Salt Reactor Experiment, MSRE)及10 MW高温气冷堆(10 MW high-temperature gas-cooled test reactor, HTR-10)与氟盐冷却球床堆的相似性进行分析,定量判断它们作为基准题的合理性。分析结果表明, MSRE和氟盐冷却球床堆的能谱峰位能量接近且堆内元素种类相近,二者相似程度较高;常温临界HTR-10和氟盐冷却球床堆冷却剂不同,且能谱峰位能量差异较大,二者相似程

  9. Inhibitors of Helicobacter pylori protease HtrA found by 'virtual ligand' screening combat bacterial invasion of epithelia.

    Directory of Open Access Journals (Sweden)

    Martin Löwer

    Full Text Available BACKGROUND: The human pathogen Helicobacter pylori (H. pylori is a main cause for gastric inflammation and cancer. Increasing bacterial resistance against antibiotics demands for innovative strategies for therapeutic intervention. METHODOLOGY/PRINCIPAL FINDINGS: We present a method for structure-based virtual screening that is based on the comprehensive prediction of ligand binding sites on a protein model and automated construction of a ligand-receptor interaction map. Pharmacophoric features of the map are clustered and transformed in a correlation vector ('virtual ligand' for rapid virtual screening of compound databases. This computer-based technique was validated for 18 different targets of pharmaceutical interest in a retrospective screening experiment. Prospective screening for inhibitory agents was performed for the protease HtrA from the human pathogen H. pylori using a homology model of the target protein. Among 22 tested compounds six block E-cadherin cleavage by HtrA in vitro and result in reduced scattering and wound healing of gastric epithelial cells, thereby preventing bacterial infiltration of the epithelium. CONCLUSIONS/SIGNIFICANCE: This study demonstrates that receptor-based virtual screening with a permissive ('fuzzy' pharmacophore model can help identify small bioactive agents for combating bacterial infection.

  10. Differential effect of DDT, DDE, and DDD on COX-2 expression in the human trophoblast derived HTR-8/SVneo cells.

    Science.gov (United States)

    Dominguez-Lopez, Pablo; Diaz-Cueto, Laura; Olivares, Aleida; Ulloa-Aguirre, Alfredo; Arechavaleta-Velasco, Fabian

    2012-11-01

    The purpose of this study was to investigate the effect of 1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane (DDT), 1,1-bis-(chlorophenyl)-2,2-dichloroethene (DDE), and 1,1-dichloro-2,2-bis(chlorophenyl)ethane (DDD) isomers on COX-2 expression in a human trophoblast-derived cell line. Cultured HTR-8/SVneo trophoblast cells were exposed to DDT isomers and its metabolites for 24 h, and COX-2 mRNA and protein expression were assessed by RT-PCR, Western blotting, and ELISA. Prostaglandin E₂ production was also measured by ELISA. Both COX-2 mRNA and protein were detected under control (unexposed) conditions in the HTR-8/SVneo cell line. COX-2 protein expression and prostaglandin E₂ production but not COX-2 mRNA levels increased only after DDE and DDD isomers exposure. It is concluded that DDE and DDD exposure induce the expression of COX-2 protein, leading to increased prostaglandin E2 production. Interestingly, the regulation of COX-2 by these organochlorines pesticides appears to be at the translational level.

  11. An overview of the FZJ-tools for HTR core design and reactor dynamics, the past, present and future

    Energy Technology Data Exchange (ETDEWEB)

    Reitsma, F. [Nuclear Engineering Analysis (NEA), PBMR (Pty Ltd), cennturion (South Africa); Rutten, H.J.; Scherer, W. [Forschungzentrum Julich GmbH, Institute for Safety Research and Reactor Technology, Julich (Germany)

    2005-07-01

    The development of the pebble-bed type high-temperature reactor in Germany was actively supported by the research centre Juelich (FZJ, former KFA) i.e. with the development of theoretical methods and computational tools to perform core neutronics design, reactor operation simulation and transient analysis. The tools, developed as the outcome of research activities, made huge contributions not only to the understanding of the technology and its physical behaviour but were also used in support of licensing of German HTR projects. Today these codes are used in the design and licensing of current commercial projects such as the Pebble Bed Modular Reactor (PBMR) but is also used to design the upcoming Gen IV reactors of HTR pebble-bed type. The renewed interest in these codes especially with respect to the pebble-bed designs is due to their unique features such as the fuel management algorithms, the simultaneous treatment of nuclear, thermal-hydraulic and fluid-dynamic problems and the description of fast and long-term transients. The paper provides an overview of the codes VSOP and TINTE, provides an update of recent developments in the codes and gives specific examples of applications such as the PBMR 400 MW running-in phase, a comparison with the SANA pebble bed effective thermal conductivity experiment and very recent results obtained simulating the corrosion due to an air ingress event simulated in the NACOK facility. Finally some ideas on the future development of these codes are discussed. (authors)

  12. KüFA safety testing of HTR fuel pebbles irradiated in the High Flux Reactor in Petten

    Energy Technology Data Exchange (ETDEWEB)

    Seeger, O., E-mail: oliver.seeger@rwth-aachen.de [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Laurie, M., E-mail: mathias.laurie@ec.europa.eu [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Abjani, A. El; Ejton, J.; Boudaud, D.; Freis, D.; Carbol, P.; Rondinella, V.V. [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Fütterer, M. [European Commission, Joint Research Centre (JRC), Institute for Energy and Transport (IET), Nuclear Reactor Integrity Assessment and Knowledge Management Unit, PO Box 2, 1755 ZG Petten (Netherlands); Allelein, H.-J. [Lehrstuhl für Reaktorsicherheit und -technik an der RWTH Aachen, Kackertstraße 9, 52072 Aachen (Germany)

    2016-09-15

    The Cold Finger Apparatus (KühlFinger-Apparatur—KüFA) in operation at JRC-ITU is designed to experimentally scrutinize the effects of Depressurization LOss of Forced Circulation (D-LOFC) accident scenarios on irradiated High Temperature Reactor (HTR) fuel pebbles. Up to 1600 °C, the reference maximum temperature for these accidents, high-quality German HTR fuel pebbles have already demonstrated a small fission product release. This paper discusses and compares the releases obtained from KüFA-testing the pebbles HFR-K5/3 and HFR-EU1/3, which were both irradiated in the High Flux Reactor (HFR) in Petten. We present the time-dependent fractional release of the volatile fission product {sup 137}Cs as well as the fission gas {sup 85}Kr for both pebbles. For HFR-EU1/3 the isotopes {sup 134}Cs and {sup 154}Eu as well as the shorter-lived {sup 110m}Ag have also been measured. A detailed description of the experimental setup and its accuracy is given. The data for the recently tested pebbles is discussed in the context of previous results.

  13. The Three Streptomyces lividans HtrA-Like Proteases Involved in the Secretion Stress Response Act in a Cooperative Manner

    Science.gov (United States)

    Vicente, Rebeca L.; Gullón, Sonia; Marín, Silvia; Mellado, Rafael P.

    2016-01-01

    Overproduction of Sec-proteins in S. lividans accumulates misfolded proteins outside of the cytoplasmic membrane where the accumulated proteins interfere with the correct functioning of the secretion machinery and with the correct cell functionality, triggering the expression in S. lividans of a CssRS two-component system which regulates the degradation of the accumulated protein, the so-called secretion stress response. Optimization of secretory protein production via the Sec route requires the identification and characterisation of quality factors involved in this process. The phosphorylated regulator (CssR) interacts with the regulatory regions of three genes encoding three different HtrA-like proteases. Individual mutations in each of these genes render degradation of the misfolded protein inoperative, and propagation in high copy number of any of the three proteases encoding genes results on indiscriminate alpha-amylase degradation. None of the proteases could complement the other two deficiencies and only propagation of each single copy protease gene can restore its own deficiency. The obtained results strongly suggest that the synthesis of the three HtrA-like proteases needs to be properly balanced to ensure the effective degradation of misfolded overproduced secretory proteins and, at the same time, avoid negative effects in the secreted proteins and the secretion machinery. This is particularly relevant when considering the optimisation of Streptomyces strains for the overproduction of homologous or heterologous secretory proteins of industrial application. PMID:27977736

  14. Computational fluid dynamic model for thermohydraulic calculation for the steady-state of the real scale HTR-1

    Energy Technology Data Exchange (ETDEWEB)

    Gamez, Abel; Rojas, Leorlen; Rosales, Jesus; Castro, Landy Y.; Gonzalez, Daniel; Garcia, Carlos, E-mail: agamezgmf@gmail.com, E-mail: leored1984@gmail.com, E-mail: jrosales@instec.cu, E-mail: lcastro@instec.cu, E-mail: danielgonro@gmail.com, E-mail: cgr@instec.cu [Instituto Superior de Tecnologias y Ciencias Aplicadas (InSTEC), La Habana (Cuba); Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Oliveira, Carlos B. de, E-mail: cabol@ufpe.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Dominguez, Dany S., E-mail: dsdominguez@gmail.com [Universidade Estadual de Santa Cruz (UESC), Ilheus, BA (Brazil)

    2015-07-01

    The high temperature gas cooled reactor (HTGR) is one of candidates of next generation of nuclear reactor according to IAEA report 2013. Evaluation of thermohydraulic performance and an experimental comparison results were proposed to the international research community. In this article, the tree dimensional CFD thermohydraulic modelation of steady state of HTR-10 modular reactor, using ANSYS CFX v14.0, has been done. Code-to-code and Code-to-experiment benchmark analyses, related to the testing program of the HTR-10 plant including steady state temperature distribution with the reactor at full power, were developed. The 3D real scale representation of reflector zone and fluid path flow inner and outer reflector blocks and cold helium cavity were carried out. The porous medium model was used to simulate the core zone in the reactor. The power distribution of the initial core published by IAEA-TECDOC-1694 obtained by Chief Scientific Investigators (CSIs) from China was used as heat sources in the core zone. (author)

  15. Valproate, thalidomide and ethyl alcohol alter the migration of HTR-8/SVneo cells

    Directory of Open Access Journals (Sweden)

    Rout Ujjwal K

    2006-08-01

    Full Text Available Abstract Background Valproate, thalidomide and alcohol (ethanol exposure during the first trimester of pregnancy is known to cause several developmental disorders. All these teratogens are known to pass the placental barrier and interfere directly with the normal development of the fetus. However, these teratogens also alter the formation and function of the placenta itself which may in turn affect the proper nourishment and development of the fetus. Optimum development of the placenta requires adequate invasion of trophoblast into the maternal uterine tissues. Changes in the migratory behavior of trophoblast by maternal exposure to these teratogens during placentogenesis may therefore alter the structure and function of the placenta. Methods In the present study, the effects of sodium valproate, thalidomide and alcohol on the migration of human first trimester trophoblast cell line (HTR-8/SVneo were examined in vitro. Cells were cultured in the wells of 48-well culture plates as mono or multilayers. Circular patches of cells were removed from the center of the wells by suction, and the migration of cells into the wound was studied using microscopy. Effects of low and high concentrations of valproate, thalidomide and alcohol were examined on the healing of wounds and on the migration rate of cells by determining the wound areas at 0, 3, 6, 12, 24 and 48 h. Effects of drugs and alcohol on the proliferation and the expression levels of integrin subunits beta1 and alpha5 in cells were examined. Results The migration rates of trophoblast differed between wounds created in mono and multilayers of cells. Exposure to teratogens altered the migration of trophoblast into mono and multilayer wounds. The effects of valproate, thalidomide and alcohol on the proliferation of cells during the rapid migratory phase were mild. Drug exposure caused significant changes in the expression levels of beta1 and alpha5 integrin subunits. Conclusion Results suggest that

  16. Gender differences in the association between HTR2C gene variants and suicidal behavior in a Mexican population: a case–control study

    Science.gov (United States)

    Molina-Guzman, Gabriel; González-Castro, Thelma Beatriz; Hernández Díaz, Yazmín; Tovilla-Zárate, Carlos Alfonso; Juárez-Rojop, Isela E; Guzmán-Priego, Crystell Guadalupe; Genis, Alma; Pool García, Sherezada; López-Narvaez, María Lilia; Rodriguez-Perez, José Manuel

    2017-01-01

    Background The aim of this case–control study was to explore the association by gender between the HTR2C gene variants and suicidal behavior in a Mexican population. Subjects and methods A total of 183 suicide attempters and 208 healthy volunteers were included in this study. We genotyped five polymorphisms of HTR2C (rs547536, rs2192372, rs4272555, rs6318, and rs2428707), then measured the association by genotype, allele, and haplotype. Results In the female group, we found an association between two polymorphisms of the HTR2C (rs4272555 and rs2428707) and suicide attempts. The C allele of the single-nucleotide polymorphism (SNP) rs4272555 was associated with a decreased risk of suicide attempt (P=0.01, odds ratio =0.26, 95% confidence interval: 0.09–0.79), whereas the G allele of the SNP rs2428707 was associated with an increased risk of suicide attempt (P=0.01, odds ratio =3.68, 95% confidence interval: 1.24–10.90). No significant association was observed between the other polymorphisms studied (rs547536, rs2192372, rs6318) or haplotypes with suicide attempts. Conclusion These findings suggest a possible risk factor of the HTR2C gene in the pathology of suicidal behavior in Mexican population. More studies are necessary to confirm this association. PMID:28260903

  17. Lithium-lithium hydride process for the production of hydrogen: comparison of two concepts for 950 and 1300 deg C HTR helium outlet temperature

    Energy Technology Data Exchange (ETDEWEB)

    Oertel, M.; Weirich, W.; Kuegler, B.; Luecke, L.; Pietsch, M.; Winkelmann, U.

    1987-01-01

    The lithium-lithium hydride process serves to generate hydrogen from water efficiently, using the high temperature heat of a nuclear reactor. Thermodynamic analyses show that hydrogen can be produced with an overall thermal efficiency of 48% at conventional HTR outlet temperatures of 950/sup 0/C. Assuming helium heat of 1300/sup 0/C, 56% overall thermal efficiency can be achieved.

  18. VENUS: cold prototype installation of the head-end of the reprocessing of HTR fuel elements. Activity report, 1 July 1976--31 December 1976

    Energy Technology Data Exchange (ETDEWEB)

    Boehnert, R.; Walter, C.

    1977-02-15

    The purpose of the VENUS Project is advance planning for the construction of a cold prototype system to incinerate HTR fuel element graphite. The Venus Project is organized into four phases between advance planning and experimental operation, corresponding to the maturity of the work. It is in the advance planning phase. Status of individual studies is given. (LK)

  19. Electroporation as a strategy to promote HtrA1 gene uptake and chemotherapy efficacy in a mouse model of mesothelioma.

    Science.gov (United States)

    Spugnini, Enrico P; Cardillo, Irene; Fanciulli, Maurizio; Crispi, Stefania; Vincenzi, Bruno; Boccellino, Mariarosaria; Quagliuolo, Lucio; Baldi, Alfonso

    2013-06-01

    There is not a consensus on the best therapeutic approach to mesothelioma and the prognosis is still dismal. We have recently demonstrated that HtrA1 is a potential therapeutic target in mesothelioma cells. In this manuscript we describe that electroporation in a mouse mesothelioma xenograft was able to facilitate the expression of exogenous HtrA1 injected intra-lesionally in the tumors and to increase the penetration in the neoplastic cells of cisplatin given intra-peritoneally. Indeed, HtrA1 over-expression caused a significant slowing down of tumor growth; moreover, cisplatin efficacy in reducing tumor mass was amplified by electroporation and this phenomenon was even more significant when combining the electroporation of cisplatin and HtrA1. Considering that a substantial number of mesothelioma patients develop early local recurrence, even with radical resection combined with aggressive chemo- and radiotherapy, this multi-modality approach could be very effective in improving local tumor control after surgery. The identification of effective combination coupled with the development of novel equipments and electrodes will be instrumental in planning the translation of these results to humans as per correct laboratory-clinical interface.

  20. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORES 9 & 10: COLUMNAR HEXAGONAL POINT-ON-POINT PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess

    2014-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  1. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORES 9 & 10: COLUMNAR HEXAGONAL POINT-ON-POINT PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess

    2013-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  2. Streptococcus pneumoniae serine protease HtrA, but not SFP or PrtA, is a major virulence factor in pneumonia.

    Science.gov (United States)

    de Stoppelaar, Sacha F; Bootsma, Hester J; Zomer, Aldert; Roelofs, Joris J T H; Hermans, Peter W M; van 't Veer, Cornelis; van der Poll, Tom

    2013-01-01

    Streptococcus (S.) pneumoniae is a common causative pathogen in pneumonia. Serine protease orthologs expressed by a variety of bacteria have been found of importance for virulence. Previous studies have identified two serine proteases in S. pneumoniae, HtrA (high-temperature requirement A) and PrtA (cell wall-associated serine protease A), that contributed to virulence in models of pneumonia and intraperitoneal infection respectively. We here sought to identify additional S. pneumoniae serine proteases and determine their role in virulence. The S. pneumoniae D39 genome contains five putative serine proteases, of which HtrA, Subtilase Family Protein (SFP) and PrtA were selected for insertional mutagenesis because they are predicted to be secreted and surface exposed. Mutant D39 strains lacking serine proteases were constructed by in-frame insertion deletion mutagenesis. Pneumonia was induced by intranasal infection of mice with wild-type or mutant D39. After high dose infection, only D39ΔhtrA showed reduced virulence, as reflected by strongly reduced bacterial loads, diminished dissemination and decreased lung inflammation. D39ΔprtA induced significantly less lung inflammation together with smaller infiltrated lung surface, but without influencing bacterial loads. After low dose infection, D39ΔhtrA again showed strongly reduced bacterial loads; notably, pneumococcal burdens were also modestly lower in lungs after infection with D39Δsfp. These data confirm the important role for HtrA in S. pneumoniae virulence. PrtA contributes to lung damage in high dose pneumonia; it does not however contribute to bacterial outgrowth in pneumococcal pneumonia. SFP may facilitate S. pneumoniae growth after low dose infection.

  3. Prolonged Activation of the Htr2b Serotonin Receptor Impairs Glucose Stimulated Insulin Secretion and Mitochondrial Function in MIN6 Cells

    Science.gov (United States)

    Cataldo, Luis Rodrigo; Mizgier, María L.; Bravo Sagua, Roberto; Jaña, Fabián; Cárdenas, César; Llanos, Paola; Busso, Dolores; Olmos, Pablo; Galgani, José E.; Santos, José L.; Cortés, Víctor A.

    2017-01-01

    Aims Pancreatic β-cells synthesize and release serotonin (5 hydroxytryptamine, 5HT); however, the role of 5HT receptors on glucose stimulated insulin secretion (GSIS) and the mechanisms mediating this function is not fully understood. The aims of this study were to determine the expression profile of 5HT receptors in murine MIN6 β-cells and to examine the effects of pharmacological activation of 5HT receptor Htr2b on GSIS and mitochondrial function. Materials and Methods mRNA levels of 5HT receptors in MIN6 cells were quantified by RT qPCR. GSIS was assessed in MIN6 cells in response to global serotonergic activation with 5HT and pharmacological Htr2b activation or inhibition with BW723C86 or SB204741, respectively. In response to Htr2b activation also was evaluated the mRNA and protein levels of PGC1α and PPARy by RT-qPCR and western blotting and mitochondrial function by oxygen consumption rate (OCR) and ATP cellular content. Results We found that mRNA levels of most 5HT receptors were either very low or undetectable in MIN6 cells. By contrast, Htr2b mRNA was present at moderate levels in these cells. Preincubation (6 h) of MIN6 cells with 5HT or BW723C86 reduced GSIS and the effect of 5HT was prevented by SB204741. Preincubation with BW723C86 increased PGC1α and PPARy mRNA and protein levels and decreased mitochondrial respiration and ATP content in MIN6 cells. Conclusions Our results indicate that prolonged Htr2b activation in murine β-cells decreases glucose-stimulated insulin secretion and mitochondrial activity by mechanisms likely dependent on enhanced PGC1α/PPARy expression. PMID:28129327

  4. HtrA1在宫颈上皮内瘤样病变及宫颈鳞癌中的表达及临床意义%Expression of HtrA1 in cervical intraepithelial neoplasm and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    宗璐; 邹余粮; 王利娟

    2012-01-01

    目的 探讨HtrA1在宫颈鳞状上皮内瘤样病变(CIN)及宫颈鳞癌中的表达及临床意义.方法 采用免疫组化SP法对10例正常宫颈、28例CIN(其中轻度8例、中度10例、重度10例)、25例原位癌及40例宫颈浸润鳞癌标本中HtrA1的表达进行检测,并与临床病理分级等进行相关分析.结果 正常宫颈组织中HtrA1无阳性表达.CIN、原位癌的阳性表达率分别为21.4%及60%,各组间差异有统计学意义(P<0.05).浸润癌阳性表达率为52.5%,较原位癌组显著降低(P<0.05).HtrA1阳性表达率与宫颈癌临床分期呈负相关(r=-0.622,P<0.05),与病理分级无关(P>0.05).无淋巴结转移者HtrA1阳性表达明显高于有淋巴结转移者(P<0.05).结论 HtrA1表达对宫颈癌的发生发展及评估预后有重要意义,可能是一种肿瘤抑制因子.%To study the expression of HtrAl in cervical intraepithelial neoplasm (CIN) and cervical squamous carcinoma and its clinical significance. Methods In 10 cases of normal cervical tissues, 28 cases of CIN (8 mild cases, 10 moderate cases and 10 severe cases), 25 cases of intraepithelial carcinoma of cervix uterus and 40 cases of invasive carcinoma of cervix uterus, the expression of HtrAl was detected by immunohistochemical method S-P. Its correlation with clinico-pathological grade was analyzed. Results HtrAl was negative in normal cervical epithelium. In CIN and intraepithelial carcinoma, the positive expressive rate of HtrAl was 21.4% and 60%, with a significant difference (P0.05) . The positive rate of HtrAl in the subjects without lymph node metastasis was much higher than that in those with lymph node metastasis (P<0. 05). Conclusion HtrAl is important in evaluating the development of neoplasm and prognosis of cervical carcinoma, and it may be an oppressor for the carcinoma.

  5. EXPERIMENTAL RESEARCH ON HTR04: A NON-OZONE DEPLETING REFRIGERANT FOR MODERATELY HIGH TEMPERATURE HEAT PUMPS%零ODP的中高温热泵工质HTR04实验研究

    Institute of Scientific and Technical Information of China (English)

    昝成; 史琳

    2007-01-01

    本文综合考虑了环保和中高温水源热泵对制冷剂的特殊要求,通过研究工质的热物理性质、材料相容性、油溶性,研发了一种臭氧层破坏潜能(ODP)为零的非共沸中高温热泵混合工质HTR04,并在中高温水源热泵试验台机组上进行了新工质的热工性能试验.实验结果表明该工质能够稳定产生80℃的热水,且有较高的性能系数,可与R134a压缩机兼容.该工质的实际工程应用前景良好.

  6. Estimation of Graphite Dust Resuspension in Steam Generator of HTR-10%HTR-10蒸汽发生器中石墨粉尘重悬浮规律预测

    Institute of Scientific and Technical Information of China (English)

    张天琦; 彭威; 于溯源

    2014-01-01

    HTR-10中石墨粉尘聚集的重要场所蒸汽发生器(SG)为对象,通过数值计算获得SG中的流动参数,在此基础上采用Vainshtein重悬浮模型计算反应堆满功率运行情况下氦气流中石墨粉尘在SG中的重悬浮份额.结果表明,沿SG高度方向,摩擦速度逐渐增加,石墨颗粒重悬浮份额随之逐渐增大;石墨粉尘颗粒粒径越大,重悬浮份额越大;石墨粉尘随时间的重悬浮规律在SG不同区域均有短期效应和长期效应.

  7. Access rules for imports and exports on the French public power transmission network; Regles d'acces au reseau public de transport francais pour des importations et des exportations

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-10-01

    These rules define the technical, financial and legal conditions applied to imports and/or exports made by the user on the French public power transmission grid (Reseau Public de Transport - RPT), considering the available capacity on interconnections and any particular technical constraints of the European interconnected network, notably in terms of safety: 1 - definitions; 2 - general conditions (scope, participation conditions, user obligations, signature, enforcement and duration of the participation agreement, attachment of transactions to a balance responsible entity, rules modification procedure, access to the information system, financial conditions, guarantee of exchange programmes, transfer of rights and obligations, intellectual property, confidentiality, force majeure, responsibility, termination, notifications and operational exchange procedures, territorial application of the rules, applicable law and language, dispute resolution); 3 - conditions for the application of imports and exports (allocating transmission capacity on interconnections, allocating import capacity, changing a capacity allocation mechanism, the different categories of exchange programmes, standard programmes, weekly programmes, daily programmes, nominations in the event of a failure in the information system, information published by RTE); 4 - conditions for the application of intra-day transactions (intra-day transaction requests, processing intra-day transaction requests, modification of an intra-day transactions programme). 16 models of correspondence documents are given in appendixes.

  8. Final Report on Utilization of TRU TRISO Fuel as Applied to HTR Systems Part I: Pebble Bed Reactors

    Energy Technology Data Exchange (ETDEWEB)

    Brian Boer; Abderrafi M. Ougouag

    2011-03-01

    significant failure is to be expected for the reference fuel particle during normal operation. It was found, however, that the sensitivity of the coating stress to the CO production in the kernel was large. The CO production is expected to be higher in DB fuel than in UO2 fuel, but its exact level has a high uncertainty. Furthermore, in the fuel performance analysis transient conditions were not yet taken into account. The effort of this task in FY 2010 has focused on the optimization of the core to maximize the pebble discharge burnup level, while retaining its inherent safety characteristics. Using generic pebble bed reactor cores, this task will perform physics calculations to evaluate the capabilities of the pebble bed reactor to perform utilization and destruction of LWR used-fuel transuranics. The task will use established benchmarked models, and will introduce modeling advancements appropriate to the nature of the fuel considered (high transuranic [TRU] content and high burn-up). Accomplishments of this work include: •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel. •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel and Uranium. •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel and Modified Open Cycle Components. •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel and Americium targets.

  9. Alternative germ cell death pathway in Drosophila involves HtrA2/Omi, lysosomes, and a caspase-9 counterpart.

    Science.gov (United States)

    Yacobi-Sharon, Keren; Namdar, Yuval; Arama, Eli

    2013-04-15

    In both flies and mammals, almost one-third of the newly emerging male germ cells are spontaneously eliminated before entering meiosis. Here, we show that in Drosophila, germ cell death (GCD) involves the initiator caspase Dronc independently of the apoptosome and the main executioner caspases. Electron microscopy of dying germ cells revealed mixed morphologies of apoptosis and necrosis. We further show that the lysosomes and their catabolic enzymes, but not macroautophagy, are involved in the execution of GCD. We then identified, in a screen, the Parkinson's disease-associated mitochondrial protease, HtrA2/Omi, as an important mediator of GCD, acting mainly through its catalytic activity rather than by antagonizing inhibitor of apoptosis proteins. Concomitantly, other mitochondrial-associated factors were also implicated in GCD, including Pink1 (but not Parkin), the Bcl-2-related proteins, and endonuclease G, which establish the mitochondria as central mediators of GCD. These findings uncover an alternative developmental cell death pathway in metazoans.

  10. Conversion of uranium nuclear fuel into U 3O 8 at the head end of HTR reprocessing

    Science.gov (United States)

    Hoogen, N.; Aschhoff, H. G.; Staib, G.

    1984-04-01

    Corresponding to the reference procedure for the head-end treatment of HTR fuel elements, separation of the moderator graphite from the materials uranium and plutonium is envisaged by combustion in the fluidized bed. Due to the defective silicon carbide layers of the uranium fuel particles a chemical conversion of the UO 2 kernel into U 3O 8 takes place in the oxidizing atmosphere of the combustion process. This reaction proceeds spontaneously and quantitatively, and causes a disintegration of the heavy metal kernel. It is observed that the degree of hardness of the kernel fragments is clearly dependent on the heat-up rate. In the commercial design of the head-end process step, attention must be paid to the cross-over of fuel from the stationary fluidized bed into the dust discharge.

  11. 儿童急性白血病促凋亡蛋白Smac、HtrA2表达的意义

    Institute of Scientific and Technical Information of China (English)

    赵东菊; 石太新; 王艳蕊; 肖爱菊; 李培岭

    2015-01-01

    目的探讨促凋亡蛋白Smac、HtrA2在儿童急性白血病(AL)中的表达,分析二者的关系及临床意义。方法77例AL患儿,其中初诊组32例,缓解组33例,复发组12例;15例非恶性血液病患儿作为对照组。采用免疫组织化学过氧化物酶标记链霉卵白素染色(SP)法检测患儿骨髓中Smac、HtrA2蛋白表达情况。应用SPSS13.0软件对结果进行分析。结果所有检测标本仅部分表达Smac,而HtrA2则在所有检测标本中均表达;初诊组患儿骨髓中Smac、HtrA2表达水平均高于缓解组(χ2=17.38、F=2.36,P均〈0.05)和对照组(χ2=12.89、F=5.26,P均〈0.05),差异均有统计学意义,但与复发组比较差异均无统计学意义(χ2=1.18、F=1.57,P均〉0.05);缓解组二者表达水平均高于对照组,但差异均无统计学意义(χ2=1.20、F=2.23,P均〉0.05)。初治AL患儿骨髓中Smac、HtrA2蛋白表达水平在急性淋巴细胞白血病(ALL)组和急性髓系自血病(AML)组比较差异均无统计学意义(χ2=0.113、t=1.024,P均〉0.05)。初诊组AL完全缓解率在Smac蛋白表达阴性(Smac-)患者组(90.9%)、HtrA2蛋白低表达(HtrA2low)患者组(84.6%)分别明显高于Smac蛋白表达阳性(Smac+)组(47.6%)、HtrA2蛋白高表达(HtrA2high)组(47.4%),差异均有统计学意义(χ2=5.772、4.596,P均〈0.05);Smac-HtrA2low表达组完全缓解率(100%)明显高于Smac+HtrA2high表达组(30.8%),差异有统计学意义(χ2=9.692,P〈0.01)。初治AL患儿骨髓中Smae、HtrA2蛋白表达水平呈正相关(r=0.979,P〈0.001)。结论促凋亡蛋白Smae、HtrA2可能参与了儿童AL的发生与病情变化过程,且二存在此过程中相互影响。在初诊患儿中二者表达水平与白血病临床类型可能无关,但Smae、HtrA2蛋白高表达提示预后不良。

  12. Quantification of human telomerase RNA (Htr) and human telomerase reverse transcriptase (Htert)Mrna in testicular tissue of infertile patients

    Institute of Scientific and Technical Information of China (English)

    Mark Schrader; Markus Miller; Ridiger Heicappell; Bernd Straub; Kurt Miller

    2001-01-01

    Aim: To evaluate the quantitative detection of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) mRNA as diagnostic parameters in the workup of testicular tissue specimens from patients presenting with non-obstructive azoospemia. Methods: hTR and hTERT mRNA expression were quantified in 38 cryopreserved testicular tissue specimens by fluorescence real-time reverse transcription- polymerase chain reaction (RT-PCR)in a LightCycler(r). This was paralleled by conventional histological workup in all tissue specimens and additional semithin sectioning preparation in cases with maturation arrest ( n = 12) and Sertoli-cell-only syndrome ( n = 12). Results: The average normalized hTERT expression (NhTERT) Was 131.9 ± 48.0 copies (mean ± SD) in tissue specimens with full spermatogenesis, NhTERT = 51.2 ± 17.2 copies in those with maturation arrest and NhTERT = 2.7 ± 2.4 copies in those with Sertoli-cell-only syndrome (SCOS). The discriminant analysis showed that detection of NhTERT (NhTR) had a predictive value of 86.8 % (55.3 % ) for correct classification in one of the three histological subgroups.Conclusion: Our results demonstrate that quantitative detection of hTERT mRNA expression in testicular tissue enables a molecular-diagnostic classification of gametogenesis. Quantitative detection of hTERT in testicular biopsies is thus well suited for supplementing the histopathological evaluation.

  13. Comparison of Homogeneous and Heterogeneous CFD Fuel Models for Phase I of the IAEA CRP on HTR Uncertainties Benchmark

    Energy Technology Data Exchange (ETDEWEB)

    Gerhard Strydom; Su-Jong Yoon

    2014-04-01

    Computational Fluid Dynamics (CFD) evaluation of homogeneous and heterogeneous fuel models was performed as part of the Phase I calculations of the International Atomic Energy Agency (IAEA) Coordinate Research Program (CRP) on High Temperature Reactor (HTR) Uncertainties in Modeling (UAM). This study was focused on the nominal localized stand-alone fuel thermal response, as defined in Ex. I-3 and I-4 of the HTR UAM. The aim of the stand-alone thermal unit-cell simulation is to isolate the effect of material and boundary input uncertainties on a very simplified problem, before propagation of these uncertainties are performed in subsequent coupled neutronics/thermal fluids phases on the benchmark. In many of the previous studies for high temperature gas cooled reactors, the volume-averaged homogeneous mixture model of a single fuel compact has been applied. In the homogeneous model, the Tristructural Isotropic (TRISO) fuel particles in the fuel compact were not modeled directly and an effective thermal conductivity was employed for the thermo-physical properties of the fuel compact. On the contrary, in the heterogeneous model, the uranium carbide (UCO), inner and outer pyrolytic carbon (IPyC/OPyC) and silicon carbide (SiC) layers of the TRISO fuel particles are explicitly modeled. The fuel compact is modeled as a heterogeneous mixture of TRISO fuel kernels embedded in H-451 matrix graphite. In this study, a steady-state and transient CFD simulations were performed with both homogeneous and heterogeneous models to compare the thermal characteristics. The nominal values of the input parameters are used for this CFD analysis. In a future study, the effects of input uncertainties in the material properties and boundary parameters will be investigated and reported.

  14. 5-HTR1A 基因在经前期综合征肝气郁证模型大鼠下丘脑区的表达%Expression of 5-HTR1A in Hypothalamus of Pre-Menstrual Syndrome Liver-qi Depression Model Rats

    Institute of Scientific and Technical Information of China (English)

    徐凯勇; 高杰; 宋春红; 李自发; 魏盛; 张惠云

    2012-01-01

    Objective To investigate the pathogenesis and drug target through the study about expression of 5-HTR1A in the hypothalamus of Pre-Menstrual Syndrome (PMS) Liver-qi Depression Model Rats. Methods The PMS Liver-qi Depression model was established by bounding the rat's feet, Then it was divided into control group, model group and treated group which were treated with Jingqianshu pellet. The mRNA and protein expressions of 5-HTR1A in the hypothalamus of the model Rats were detected by RT-PCR and Western Blotting separately. The data were analyzed by the one-way ANOVA and LSD with the software SPSS 16.0. Results The mRNA and protein expressions of 5-HTR1A in the hypothalamus of model group were decreased significantly compared with the control group. And the expressions of the treated group with Jingqianshu pellet were increased significantly compared with the model group. But there were no significant difference between the treated and the control groups. Conclusion The expressions of 5-HTR1A in the hypothalamus of PMS Liver-qi Depression Model Rats were decreased significantly compared with the control rats, and then the expressions level could recovered to the control level after treated with Jingqianshu pellet. Therefore, it was demonstrated that the expression of 5-HTR1A and PMS Liver-qi Depression may related, and the gene could be the drug target of Jingqianshu granule.%目的 通过研究5-羟色胺受体1A (5-HTR1A)基因在经前期综合征(PMS)肝气郁证模型大鼠下丘脑区的表达,探讨该证的发病机制及药物作用靶点.方法 束缚法制备PMS肝气郁证模型大鼠,经前舒颗粒干预,获得正常组、造模组、给药组;运用RT-PCR和Western Blotting方法检测大鼠下丘脑区5-HTR1A mRNA及其蛋白表达;应用软件SPSS 16.0,通过单因素方差分析及LSD法统计数据.结果 PMS肝气郁证模型组大鼠与正常组相比,下丘脑5-HTR1A mRNA及蛋白表达均显著降低;经前舒颗粒给药后与造模组相比,

  15. Expression and Immunogenicity of a Mutant Diphtheria Toxin Molecule, CRM197, and Its Fragments in Salmonella typhi Vaccine Strain CVD 908-htrA

    Science.gov (United States)

    Orr, Nadav; Galen, James E.; Levine, Myron M.

    1999-01-01

    Mutant diphtheria toxin molecule CRM197 and fragments thereof were expressed in attenuated Salmonella typhi CVD 908-htrA, and the constructs were tested for their ability to induce serum antitoxin. Initially, expressed proteins were insoluble, and the constructs failed to induce neutralizing antitoxin. Soluble CRM197 was expressed at low levels by utilizing the hemolysin A secretion system from Escherichia coli. PMID:10417208

  16. Diversity of the 47-kD HtrA nucleic acid and translated amino acid sequences from 17 recent human isolates of Orientia.

    Science.gov (United States)

    Jiang, Ju; Paris, Daniel H; Blacksell, Stuart D; Aukkanit, Nuntipa; Newton, Paul N; Phetsouvanh, Rattanaphone; Izzard, Leonard; Stenos, John; Graves, Stephen R; Day, Nicholas P J; Richards, Allen L

    2013-06-01

    Orientia tsutsugamushi, the etiologic agent of potentially fatal scrub typhus, is characterized by a high antigenic diversity, which complicates the development of a broadly protective vaccine. Efficacy studies in murine and nonhuman primate models demonstrated the DNA vaccine candidate pKarp47, based upon the O. tsutsugamushi Karp 47-kD HtrA protein gene, to be a successful immunoprophylactic against scrub typhus. To characterize 47-kD HtrA protein diversity among human isolates of Orientia, we sequenced the full open reading frame (ORF) of the 47-kD HtrA gene and analyzed the translated amino acid sequences of 17 patient isolates from Thailand (n=13), Laos (n=2), Australia (n=1), and the United Arab Emirates (UAE) (n=1) and 9 reference strains: Karp (New Guinea), Kato (Japan), Ikeda (Japan), Gilliam (Burma), Boryong (Korea), TA763, TH1811 and TH1817 (Thailand), and MAK243 (China). The percentage identity (similarity) of translated amino acid sequences between 16 new isolates and 9 reference strains of O. tsutsugamushi ranged from 96.4% to 100% (97.4% to 100%). However, inclusion of the recently identified Orientia chuto sp. nov. reduced identity (similarity) values to 82.2% to 83.3% (90.4% to 91.4%). These results demonstrate the diversity of Orientia 47-kD HtrA among isolates encountered by humans and therefore provide support for the necessity of developing a broadly protective scrub typhus vaccine that takes this diversity into account.

  17. Numerical Analysis of Magnetically Suspended Rotor in HTR-10 Helium Circulator Being Dropped into Auxiliary Bearings%HTR-10氦风机磁悬浮转子跌落在辐助轴承上的数值分析

    Institute of Scientific and Technical Information of China (English)

    赵泾雄; 杨国军; 李悦; 于溯源

    2012-01-01

    Active magnetic bearings (AMB) have been selected to support the rotor of primary helium circulator in commercial 10 Mega-Walt High Temperature Gas-cooled Reactor (HTR-10). In an AMB system, the auxiliary bearings are necessary to protect the AMB components in case of losing power. This paper performs the impact simulation of Magnetically Suspended Rotor in HTR-10 Helium Circulator being dropped into the auxiliary bearings using the finite element program ABAQUS. The dynamic response and the strain field of auxiliary bearings are analyzed. The results achieved by the numerical analysis are in agreement with the experiment results. Therefore, the feasibility of the design of auxiliary bearing and the possibility of using the AMB system in the HTR are proved.%辅助轴承是10 MW高温气冷堆(HTR-10)氦风机磁力轴承-转子系统中的辅助支承结构,其主要任务是在磁力轴承因失电而失效时支承高速转动的转子,是整个系统安全运行的保证.本研究以HTR-10磁力轴承氦风机实验台架中的辅助轴承为研究对象,使用ABAQUS有限元软件数值模拟转子跌落,分析滚动辅助轴承内圈与滚动体的变形及能量损耗特性,并与初步的实验结果进行比对,验证辅助轴承的可靠性,为磁力轴承在高温气冷堆核电厂中的应用提供重要的技术保障.

  18. The Shandong Shidao Bay 200 MWe High-Temperature Gas-Cooled Reactor Pebble-Bed Module (HTR-PM Demonstration Power Plant: An Engineering and Technological Innovation

    Directory of Open Access Journals (Sweden)

    Zuoyi Zhang

    2016-03-01

    Full Text Available After the first concrete was poured on December 9, 2012 at the Shidao Bay site in Rongcheng, Shandong Province, China, the construction of the reactor building for the world's first high-temperature gas-cooled reactor pebble-bed module (HTR-PM demonstration power plant was completed in June, 2015. Installation of the main equipment then began, and the power plant is currently progressing well toward connecting to the grid at the end of 2017. The thermal power of a single HTR-PM reactor module is 250 MWth, the helium temperatures at the reactor core inlet/outlet are 250/750 °C, and a steam of 13.25 MPa/567 °C is produced at the steam generator outlet. Two HTR-PM reactor modules are connected to a steam turbine to form a 210 MWe nuclear power plant. Due to China's industrial capability, we were able to overcome great difficulties, manufacture first-of-a-kind equipment, and realize series major technological innovations. We have achieved successful results in many aspects, including planning and implementing R&D, establishing an industrial partnership, manufacturing equipment, fuel production, licensing, site preparation, and balancing safety and economics; these obtained experiences may also be referenced by the global nuclear community.

  19. Thorium utilization in a small long-life HTR. Part II: Seed-and-blanket fuel blocks

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Ming, E-mail: dingming@hrbeu.edu.cn [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB Delft (Netherlands); Harbin Engineering University, Nantong Street 145, 150001 Harbin (China); Kloosterman, Jan Leen [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB Delft (Netherlands)

    2014-02-15

    Highlights: • Seed-and-blanket (S and B) fuel blocks are proposed for a small block-type HTR. • S and B fuel blocks consist of a seed region (UO{sub 2}) and a blanket region (ThO{sub 2}). • The neutronic performance of S and B fuel blocks are analyzed using SCALE 6. • Three S and B fuel blocks with a reactivity swing of 0.1 Δk are recommended. • S and B fuel blocks are compared with thorium MOX fuel blocks. - Abstract: In order to utilize thorium in high temperature gas-cooled reactors (HTRs), the concept of seed-and-blanket (S and B) fuel block is introduced into the U-Battery, which is a long-life block-type HTR with a thermal power of 20 MWth. A S and B fuel block consists of a seed region with uranium in the center, and a blanket region with thorium. The neutronic performance, such as the multiplication factor, conversion ratio and reactivity swing, of a typical S and B fuel block was investigated by SCALE 6.0 by parametric analysis of the composition parameters and geometric parameters of the fuel block for the U-Battery application. Since the purpose of U-235 in the S and B fuel block is to ignite the fission reactions in the fuel block, 20% enriched uranium is recommended for the S and B fuel block. When the ratio of the number of carbon to heavy metal atoms changes with the geometric parameters of the fuel block in the range of 200–250, the reactivity swing reaches very small values. Furthermore, for a reactivity swing of 0.1 Δk during 10 effective full power years, three configurations with 36, 54 and 78 UO{sub 2} fuel rods are recommended for the application of the U-Battery. The comparison analysis of the S and B fuel block with the Th/U MOX fuel block shows that the former has a longer lifetime and a lower reactivity swing.

  20. Thorium utilization in a small long-life HTR. Part I: Th/U MOX fuel blocks

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Ming, E-mail: dingm2005@gmail.com [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB, Delft (Netherlands); Harbin Engineering University, Nantong Street 145, 150001 Harbin (China); Kloosterman, Jan Leen, E-mail: j.l.kloosterman@tudelft.nl [Delft University of Technology, Reactor Institute Delft, Mekelweg 15, 2629 JB, Delft (Netherlands)

    2014-02-15

    Highlights: • We propose thorium MOX (TMOX) fuel blocks for a small block-type HTR. • The TMOX fuel blocks with low-enriched uranium are recommended. • More thorium decreases the reactivity swing of the TMOX fuel blocks. • Thorium reduces the negative temperature coefficient of the TMOX fuel blocks. • Thorium increases the conversion ratio of the TMOX fuel blocks. - Abstract: The U-Battery is a small, long-life and transportable high temperature gas-cooled reactor (HTR). The neutronic features of a typical fuel block with uranium and thorium have been investigated for a application of the U-Battery, by parametrically analyzing the composition and geometric parameters. The type of fuel block is defined as Th/U MOX fuel block because uranium and thorium are assumed to be mixed in each fuel kernel as a form of (Th,U)O{sub 2}. If the initially loaded mass of U-235 is mostly consumed in the early period of the lifetime of Th/U MOX fuel block, low-enriched uranium (LEU) as ignited fuel will not largely reduce the neutronic performance of the Th/U MOX fuel block, compared with high-enriched uranium. The radii of fuel kernels and fuel compacts and packing fraction of TRISO particles determine the atomic ratio of the carbon to heavy metal. When the ratio is smaller than 400, the difference among them due to double heterogeneous effects can be neglected for the Th/U MOX fuel block. In the range between 200 and 400, the reactivity swing of the Th/U MOX fuel block during 10 years is sufficiently small. The magnitude of the negative reactivity temperature coefficients of the Th/U MOX fuel block decreases by 20–45%, which is positive to reduce temperature defect of the Th/U MOX fuel block. The conversion ratio (CR) of the fuel increases from 0.48 (typical CR of the LEU-fueled U-Battery) to 0.78. The larger conversion ratio of the Th/U MOX fuel block reduces the reactivity swing during 10 years for the U-Battery.

  1. GenHtr: a tool for comparative assessment of genetic heterogeneity in microbial genomes generated by massive short-read sequencing

    Directory of Open Access Journals (Sweden)

    Yu GongXin

    2010-10-01

    Full Text Available Abstract Background Microevolution is the study of short-term changes of alleles within a population and their effects on the phenotype of organisms. The result of the below-species-level evolution is heterogeneity, where populations consist of subpopulations with a large number of structural variations. Heterogeneity analysis is thus essential to our understanding of how selective and neutral forces shape bacterial populations over a short period of time. The Solexa Genome Analyzer, a next-generation sequencing platform, allows millions of short sequencing reads to be obtained with great accuracy, allowing for the ability to study the dynamics of the bacterial population at the whole genome level. The tool referred to as GenHtr was developed for genome-wide heterogeneity analysis. Results For particular bacterial strains, GenHtr relies on a set of Solexa short reads on given bacteria pathogens and their isogenic reference genome to identify heterogeneity sites, the chromosomal positions with multiple variants of genes in the bacterial population, and variations that occur in large gene families. GenHtr accomplishes this by building and comparatively analyzing genome-wide heterogeneity genotypes for both the newly sequenced genomes (using massive short-read sequencing and their isogenic reference (using simulated data. As proof of the concept, this approach was applied to SRX007711, the Solexa sequencing data for a newly sequenced Staphylococcus aureus subsp. USA300 cell line, and demonstrated that it could predict such multiple variants. They include multiple variants of genes critical in pathogenesis, e.g. genes encoding a LysR family transcriptional regulator, 23 S ribosomal RNA, and DNA mismatch repair protein MutS. The heterogeneity results in non-synonymous and nonsense mutations, leading to truncated proteins for both LysR and MutS. Conclusion GenHtr was developed for genome-wide heterogeneity analysis. Although it is much more time

  2. HTR-PM燃料元件生产穿衣工艺及设备研制%Overcoating Process and Equipments Development in Manufacture of Fuel Elements for HTR-PM

    Institute of Scientific and Technical Information of China (English)

    卢振明; 周湘文; 张杰; 刘兵

    2012-01-01

    The equipments used in the overcoating process was developed, which comprise overcoating system, rotating screen, sorting device, homogenize equipment, and recovery system for unqualified overcoated particles. The results of batch experiments using optimized parameters show that the yield is high and stable. The failure ratio of coated particles in fuel elements satisfies design parameter. The equipments are easy to operation and control, and can satisfy the large-scale production of overcoated particles of fuel elements for HTR-PM.%自主研制了穿衣工艺所用非标设备,包括穿衣系统、滚筒筛、振选台、均匀化设备、不合格颗粒回收系统等.工艺实验结果显示,穿衣颗粒成品率高且稳定,破损率满足设计要求.设备易于操作控制,完全能满足高温气冷堆示范电站燃料元件规模生产的需要.

  3. 不同龋敏感儿童口腔变异链球菌临床分离株与 HtrA 关系的研究%Evaluation of the relationship between HtrA and streptococcus mutans isolated from the children with different caries experience *

    Institute of Scientific and Technical Information of China (English)

    杨江华; 郭夕源; 王光平; 李明霞; 刘兴容

    2013-01-01

    Objective To evaluate the relationship between mRNA and protein expression of HtrA and Streptococcus mutans i-solated from the children with different caries experience and to provide the theoretical and experimental basis on prediction of dent-al caries in deciduous teeth .Methods The strains of Streptococcus mutans isolated from children with different carious experiences in the preliminary experiments were divided into three groups :high caries-susceptible group ,middle caries-susceptible group ,caries-free group .All strains were reanimated on the agar plate of MS ,and after smear pure culture examination ,typical bacteria were in-cubated in BHI ,then purified nucleic acid and extracted all the RNA of streptococcus mutans by reverse transcription PCR and de-tected it by agarose gel electrophoresis integrality .Synthetic cDNA and take further PCR amplification with cDNA products .Ob-serve records results by Gel imaging system .HtrA of target gene and electrophoresis image were gray scan by Gel quantitative soft-ware Gel-Pro analyzer 4 .0 was used to analyze relative expression value of gene .After purifying protein ,collected total protein of Streptococcus mutans strains by Western Blot method ,then tested the concentration of total protein sample .The results of Chemilu-minescence imaging were scanned into computer by Bio-Rad analyzing system ,calculated the gray value by software Quantity One 4 .4 .0 which showed the relative expression level of protein .Results There were significant differences in HtrA mRNA and protein expression of different Streptococcus mutans isolated from the children with different caries susceptibility .high caries-susceptible group> middle caries-susceptible group> caries-free group (P< 0 .05) .Conclusion There were significant differences in HtrA mRNA and protein expression of different Streptococcus mutans isolated from the children with different caries susceptibility .The higher caries susceptibility the group was ,the more Htr

  4. Les soubresauts de la fusion froide

    CERN Multimedia

    Augereau, J F

    1993-01-01

    "En 1989, deux chercheurs avaient affirmé avoir découvert une nouvelle source d'énergie. Malgré le scepticisme de la communauté scientifique, ils persistent et signent un nouvel article sur la sujet".

  5. Expression of Omi/HtrA2 in Human Renal Proximal Tubular Epithelial Cells with Hypoxia/Reoxygenation and Effect of Erythropoietin on It%Omi/HtrA2在缺氧复氧后人近曲肾小管上皮细胞中的表达及促红细胞生成素对其表达的影响

    Institute of Scientific and Technical Information of China (English)

    邹礼乐; 徐富翠; 梅欣明; 雷小平; 董文斌

    2012-01-01

    目的 探讨缺氧复氧(H/R)后人近曲肾小管上皮细胞内Omi/HtrA2的表达变化及促红细胞生成素(EPO)干预的影响.方法 以人近曲肾小管上皮细胞株(HK-2细胞)为研究对象,将其分为对照组、H/R组及EPO干预组.对照组常规培养;H/R组缺氧24h后复氧6 h;EPO干预组在H/R前加入5 000 U·L-1EPO预处理.倒置显微镜观察细胞形态,四甲基偶氮唑蓝(MTT)法检测细胞活力,流式细胞仪检测细胞凋亡,免疫组织化学检测细胞内Omi/HtrA2表达变化.结果 与对照组比较,H/R组细胞数量减少,细胞形态发生改变,细胞活力下降,细胞凋亡率显著增加,细胞内Omi/HtrA2表达增强,差异均有统计学意义(Pa<0.05);而与H/R组相比,EPO干预组细胞数量增多,细胞形态明显改善,细胞活力增加,细胞凋亡率下降,Omi/HtrA2表达减弱,差异均有统计学意义(Pa<0.05).但各指标均未恢复至对照组水平.结论.H/R可通过上调Omi/HtrA2表达而促进肾小管上皮细胞凋亡,EPO对H/R肾小管上皮细胞具有保护作用,其机制可能与抑制Omi/HtrA2表达、减少细胞凋亡有关.%Objective To investigate the expression of Omi/HtrA2 in renal tubular epithelial cells with hypoxia /reoxygenation( H/R) and to evaluate the effect of erythropoietin ( EPO) on it. Methods Human renal proximal tubular epithelial cell line HK - 2 cell was used as target cell. The cultural cells in orifice were divided into the following groups: control group, H/R group and EPO group. The cells of control group were cultivated with normal nutritive medium; the cells of the H/R group were exposed to hypoxia for 24 h.then to normoxia for 6 h;and the cells of EPO group were added 5 000 U · L-1 EPO before H/R. The change of morphology was observed under inverted microscope,the cell viability was measured by 3 - (4,5 ) - dimethylthiahiazo ( -z-yl) -3,5 - di - phenytetrazoliumromide ( MTT) method. The rate of apoptosis cells was detected by flow cytometry

  6. 愤怒、郁怒反应模型大鼠海马5-HTR2C的基因表达%The expression of 5-HTR2C in the hippocampal of the anger-in and anger-out animal model

    Institute of Scientific and Technical Information of China (English)

    王杰琼; 张惠云

    2011-01-01

    目的 怒是影响人类健康的重要情志因素,分为愤怒和郁怒两种表达方式,两者诱发病证的机制尚未完全明确.该文主要探索愤怒、郁怒情志反应的微观机制.方法 采用居住入侵和社会隔离的方法制备愤怒、郁怒反应大鼠模型,并分别予以调肝方药经前平颗粒、经前舒颗粒干预,以正常大鼠作为对照,分别取愤怒、郁怒模型组以及经前平、经前舒给药组大鼠的海马用以基因芯片检测,初步筛选出与愤怒、郁怒密切相关的基因5-HTR2C、GABABR2和5-HTR3B,运用RT-PCR和Western blot技术仅对海马中基因5-HTR2C进一步进行检测.结果 与正常组相比,5-HTR2C mRNA和蛋白表达在愤怒、郁怒反应大鼠海马中均增高(P<0.01),而且愤怒组的改变程度明显大于郁怒组,上述改变在模型用药组均得到不同程度的改善,基本可恢复到正常水平.结论 愤怒、郁怒情志刺激可导致海马基因5-HTR2C的表达水平改变,调肝方药在一定程度上可缓解上述情况,说明5-HTR2C与怒密切相关,且与愤怒关系更加密切,以上结果为下一步的研究提供方向和线索.%Aim Anger is an important emotion response affecting human health, which is divided into anger-in and anger-out.But the mechanism of disease and syndrome induced both anger-in and anger-out is not yet fully clear.So the aim of the paper is to explore micro-mechanism of disease and syndrome induced both anger-in and anger-out.Methods The anger-in and anger-out animal models were prepared using a social isolation plus resident-intruder stress.The rats were treated by" tiao gan fang yao"Jingqianping granule and Jingqianshu granule.Normal rats as control.Genes closely related with anger-in and anger-out were initially screened utilizing Gene chip to detect hippocampus of other groups.The genes were 5-HTR2C, GABABR2 and 5-HTR3B.Futher 5-HTR2C in hippocampus moving was detected by RT-PCR and Western blot in the paper

  7. Family-based study of HTR2A in suicide attempts: observed gene, gene × environment and parent-of-origin associations.

    Science.gov (United States)

    Ben-Efraim, Y J; Wasserman, D; Wasserman, J; Sokolowski, M

    2013-07-01

    While suicidal behavior is frequently accompanied by serotonergic system alterations, specific associations with genetic variation in the serotonin 2A receptor (HTR2A) gene have been inconsistent. Using a family-based study design of 660 offspring who have made a suicide attempt (SA) and both parents, we conducted an association and linkage analysis using single-nucleotide polymorphisms (SNPs) with extensive gene coverage, and included the study of parent-of-origin (POE) and gene-environment interaction (G × E), also using previously unstudied exposures. The main finding was a G × E between the exon 1 SNP rs6313 and exposure to cumulative types of lifetime stressful life events (SLEs), driven by overtransmission of CT and undertransmission of TT, both in relation to other genotypes. Further exploratory analysis revealed a significant POE in this G × E in female subjects, which followed a polar overdominant inheritance pattern. In addition, rs6310 and rs6305 were found to significantly associate with SA in the total sample. A G × E in female subjects (rs7322347 × physical assault in childhood/adolescence) confirmed features of a previously observed association with SA. Other potentially interesting nominally significant findings were observed, but like the G × E of rs7322347 did not pass a false-discovery rate cutoff. Taken together, this study found multiple associations of HTR2A SNPs on SA, with strongest statistical evidence for a G × E involving rs6313, and further suggested the importance of taking into account different inheritance patterns and G × Es with regard to HTR2A.

  8. Genome-wide association study of theta band event-related oscillations identifies serotonin receptor gene HTR7 influencing risk of alcohol dependence.

    Science.gov (United States)

    Zlojutro, Mark; Manz, Niklas; Rangaswamy, Madhavi; Xuei, Xiaoling; Flury-Wetherill, Leah; Koller, Daniel; Bierut, Laura J; Goate, Alison; Hesselbrock, Victor; Kuperman, Samuel; Nurnberger, John; Rice, John P; Schuckit, Marc A; Foroud, Tatiana; Edenberg, Howard J; Porjesz, Bernice; Almasy, Laura

    2011-01-01

    Event-related brain oscillations (EROs) represent highly heritable neuroelectrical correlates of human perception and cognitive performance that exhibit marked deficits in patients with various psychiatric disorders. We report the results of the first genome-wide association study (GWAS) of an ERO endophenotype-frontal theta ERO evoked by visual oddball targets during P300 response in 1,064 unrelated individuals drawn from a study of alcohol dependence. Forty-two SNPs of the Illumina HumanHap 1 M microarray were selected from the theta ERO GWAS for replication in family-based samples (N = 1,095), with four markers revealing nominally significant association. The most significant marker from the two-stage study is rs4907240 located within ARID protein 5A gene (ARID5A) on chromosome 2q11 (unadjusted, Fisher's combined P = 3.68 × 10⁻⁶). However, the most intriguing association to emerge is with rs7916403 in serotonin receptor gene HTR7 on chromosome 10q23 (combined P = 1.53 × 10⁻⁴), implicating the serotonergic system in the neurophysiological underpinnings of theta EROs. Moreover, promising SNPs were tested for association with diagnoses of alcohol dependence (DSM-IV), revealing a significant relationship with the HTR7 polymorphism among GWAS case-controls (P = 0.008). Significant recessive genetic effects were also detected for alcohol dependence in both case-control and family-based samples (P = 0.031 and 0.042, respectively), with the HTR7 risk allele corresponding to theta ERO reductions among homozygotes. These results suggest a role of the serotonergic system in the biological basis of alcohol dependence and underscore the utility of analyzing brain oscillations as a powerful approach to understanding complex genetic psychiatric disorders.

  9. Association between variants of 5-hydroxytryptamine receptor 3C (HTR3C) and chemotherapy-induced symptoms in women receiving adjuvant treatment for breast cancer.

    Science.gov (United States)

    Pud, Dorit; Har-Zahav, Gil; Laitman, Yael; Rubinek, Tami; Yeheskel, Adva; Ben-Ami, Sarah; Kaufman, Bella; Friedman, Eitan; Symon, Zvi; Wolf, Ido

    2014-02-01

    Administration of chemotherapy is associated with a wide array of symptoms affecting quality of life. Genetic risk factors for severity of chemotherapy-induced symptoms have not been determined. The present study aimed to explore the associations between polymorphisms in candidate genes and chemotherapy-induced symptoms. Women treated with at least two cycles of adjuvant doxorubicin and cyclophosphamide, with or without paclitaxel for early breast cancer (n = 105) completed the memorial symptom assessment scale and provided blood for genotyping. DNA was extracted from peripheral blood leukocytes and assayed for single nucleotide polymorphisms (SNPs) in GTP cyclohydrolase 1 (GCH1, rs10483639, rs3783641, and rs8007267), catecholamine-o-methyltransferase (COMT, rs4818), and 5-hydroxytryptamine (serotonin) receptor 3C (HTR3C, rs6766410, and rs6807362). Genotyping of HTR3C revealed a significant association between the presence of rs6766410 and rs6807362 SNPs (K163 and G405 variants) and increased severity of symptoms (p = 0.0001 and p = 0.007, respectively). Multiple regressions revealed that rs6766410 and rs6807362, but not age or stage at diagnosis, predicted severity of symptoms (p = 0.001 and p = 0.006, respectively) and explained 12 % of the variance in each regression model. No association was found between the genetic variants of CGH1 or COMT and symptom score. Our study indicates, for the first time, an association between variants of HTR3C and severity of chemotherapy-induced symptoms. Analyzing these genetic variants may identify patients at increased risk for the development of chemotherapy-induced symptoms and targeting the serotonin pathway may serve as a novel treatment strategy for these patients.

  10. The presence of both serotonin 1A receptor (HTR1A and dopamine transporter (DAT1 gene variants increase the risk of borderline personality disorder

    Directory of Open Access Journals (Sweden)

    Peter R Joyce

    2014-01-01

    Full Text Available Dysfunction in the dopaminergic and serotonergic neurotransmitter systems has been demonstrated to be important in the aetiology of Borderline personality disorder (BPD. We investigated the relationship of two BPD risk factors, the HTR1A promoter polymorphism -1019C>G (rs6295 and the DAT1 repeat allele, with BPD in a major depressive disorder cohort of 367 patients. Out-patients with major depressive disorder were recruited for two treatment trials and assessed for personality disorders, including BPD. DNA samples were collected and the rs6295 polymorphism was detected with a TaqMan® assay. The DAT1 repeat allele was genotyped using a modified PCR method. The impact of polymorphisms on BPD was statistically analysed using uncontrolled logistic and multiple logistic regression models. BPD patients had higher frequencies of the DAT1 9,9 (OR=2.67 and 9,10 (OR=3.67 genotypes and also those homozygous HTR1A G allele (OR=2.03. No significant interactions between HTR1A and DAT1 genotypes, were observed; however, an increased risk of BPD was observed for those patients who were either 9,10; G,G (OR=6.64 and 9,9; C,G (OR=5.42. Furthermore, the odds of BPD in patients exhibiting high-risk variants of these two genes differed from those of patients in low-risk groups by up to a factor of 9. Our study provides evidence implicating the importance of the serotonergic and dopaminergic systems in BPD and that the interaction between genes from different neurotransmitters may play a role in the susceptibility to BPD.

  11. Association between HTR2A T102C polymorphism and major depressive disorder: a meta-analysis in the Chinese population.

    Science.gov (United States)

    Lin, Chun-Xia; Hu, Zhe; Yan, Ze-Ming; Li, Wen; Chen, Yu-Sen; Zhao, Jiang-Hao; Zhang, Liang-Qing; Zhao, Bin; Zhong, Wang-Tao; Feng, Du

    2015-01-01

    Although a number of studies have been conducted on the association between HTR2A T102C polymorphism and major depressive disorder (MDD) in Chinese, this association remains elusive and controversial. To clarify the effects of HTR2A T102C polymorphism on the risk of MDD, a meta-analysis was performed in the Chinese population. Related studies were identified from PubMed, Springer Link, Ovid, Chinese Wanfang Data Knowledge Service Platform, Chinese National Knowledge Infrastructure (CNKI), and Chinese Biology Medicine (CBM) till 5 May 2015. The odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the strength of the associations. Statistical analyses were conducted with Version 10.0 STATA statistical software. A total of 12 case-control studies including 1444 MDD cases and 1445 controls were involved in this meta-analysis. Overall, no significant association with MDD risk was provided in the Chinese population (C vs. T: OR=0.97, 95% CI: 0.81-1.17, 95%; CC vs. TT: OR=0.95, 95% CI: 0.65-1.37; CC+TC vs. TT: OR=0.96, 95% CI: 0.75-1.12; CC vs. TT+TC: OR=0.94, 95% CI: 0.78-1.12). In subgroup analyses stratified by geographic area and source of controls, no significant association was found in any of the subgroups. In conclusion, this meta-analysis indicate that the HTR2A T102C polymorphism is not associated with susceptibility to MDD in Chinese population.

  12. Polymorphism in the serotonin receptor 2a (HTR2A gene as possible predisposal factor for aggressive traits.

    Directory of Open Access Journals (Sweden)

    Zsofia Banlaki

    Full Text Available Aggressive manifestations and their consequences are a major issue of mankind, highlighting the need for understanding the contributory factors. Still, aggression-related genetic analyses have so far mainly been conducted on small population subsets such as individuals suffering from a certain psychiatric disorder or a narrow-range age cohort, but no data on the general population is yet available. In the present study, our aim was to identify polymorphisms in genes affecting neurobiological processes that might explain some of the inter-individual variation between aggression levels in the non-clinical Caucasian adult population. 55 single nucleotide polymorphisms (SNP were simultaneously determined in 887 subjects who also filled out the self-report Buss-Perry Aggression Questionnaire (BPAQ. Single marker association analyses between genotypes and aggression scores indicated a significant role of rs7322347 located in the HTR2A gene encoding serotonin receptor 2a following Bonferroni correction for multiple testing (p = 0.0007 both for males and females. Taking the four BPAQ subscales individually, scores for Hostility, Anger and Physical Aggression showed significant association with rs7322347 T allele in themselves, while no association was found with Verbal Aggression. Of the subscales, relationship with rs7322347 was strongest in the case of Hostility, where statistical significance virtually equaled that observed with the whole BPAQ. In conclusion, this is the first study to our knowledge analyzing SNPs in a wide variety of genes in terms of aggression in a large sample-size non-clinical adult population, also describing a novel candidate polymorphism as predisposal to aggressive traits.

  13. Effect of polysaccharides extract of rhizoma atractylodis macrocephalae on thymus, spleen and cardiac indexes, caspase-3 activity ratio, Smac/DIABLO and HtrA2/Omi protein and mRNA expression levels in aged rats.

    Science.gov (United States)

    Guo, Ling; Sun, Yong Le; Wang, Ai Hong; Xu, Chong En; Zhang, Meng Yuan

    2012-10-01

    This study was designed to determine the possible protective effect of polysaccharides extract of rhizoma atractylodis macrocephalae on heart function in aged rats. Polysaccharides extract of rhizoma atractylodis macrocephalae was administered to aged rats. Results showed that thymus, spleen and cardiac indexs were significantly increased, whereas caspase-3 activity ratio, Smac/DIABLO and HtrA2/Omi protein expression, Smac/DIABLO and HtrA2/Omi mRNA expression levels were markedly reduced. It can be concluded that polysaccharides extract of rhizoma atractylodis macrocephalae may enhance immunity and improve heart function in aged rats.

  14. Spatially resolved modelling of the fission product behaviour in a HTR-core with spherical or prismatic fuel elements; Raeumlich hoch aufgeloeste Modellierung des Spaltproduktverhaltens in einem HTR-Core mit kugelfoermigen oder prismatischen Brennelementen

    Energy Technology Data Exchange (ETDEWEB)

    Xhonneux, Andre

    2014-07-01

    One of the most important aspects during the licensing procedure of nuclear facilities is the release of radioactive isotopes. The transport from the origin to the environment is called release chain. In the scope of this work, the spatially distributed fission product release from both spherical and prismatic fuel elements, the transport with the coolant as well as the deposition on reactor internals are simulated in detail. The fission product release codes which were developed at Forschungszentrum Juelich are analyzed, shortcomings are identified and resolved. On this basis, a consistent simulation module, named STACY, was developed, which contains all capabilities of the stand-alone codes and at the same time exceeds the methodology towards new aspects. The physics models were extended, for example to take the radial temperature profile within the fuel element and the realistic time-depending nuclide inventory into account. A central part of this work is the automated treatment of the release behavior of a representative number of fuel elements. This allows for a spatially resolved release calculation, where an individual release rate is calculated for each space region. The coupling with the depletion code Topological Nuclide Transmutation (TNT) allows for conducting an individual depletion calculation for each considered fuel element. It is shown, that the released inventory is representative for a certain number of fuel elements. By using this model, the fission product release is being studied for a reference plant (HTR-Modul). Both the releases from the equilibrium core as well as the release during a core heat-up after a fast depressurization accident are being studied. In comparison to former studies, the cumulative release of long-lived nuclides during the core heat-up phase is lower and the release of short-lived nuclides is about two times higher. The release calculation can also be conducted for prismatic fuel elements (e.g. those of the Japanese

  15. Blocking Epidermal Growth Factor Receptor Signaling in HTR-8/SVneo First Trimester Trophoblast Cells Results in Dephosphorylation of PKBα/AKT and Induces Apoptosis

    Directory of Open Access Journals (Sweden)

    J. Bolnick

    2011-01-01

    Full Text Available We identified a major peptide signaling target of EGF/EGFR pathway and explored the consequences of blocking or activating this pathway in the first trimester extravillous trophoblast cells, HTR-8/SVneo. A global analysis of protein phosphorylation was undertaken using novel technology (Kinexus Kinetworks that utilizes SDS-polyacrylamide minigel electrophoresis and multi-lane immunoblotting to permit specific and semiquantitative detection of multiple phosphoproteins. Forty-seven protein phosphorylation sites were queried, and the results reported based on relative phosphorylation at each site. EGF- and Iressa-(gefitinib, ZD1839, an inhibitor of EGFR treated HTR-8/SVneo cells were subjected to immunoblotting and flow cytometry to confirm the phosphoprotein screen and to assess the effects of EGF versus Iressa on cell cycle and apoptosis. EGFR mediates the phosphorylation of important signaling proteins, including PKBα/AKT. This pathway is likely to be central to EGFR-mediated trophoblast survival. Furthermore, EGF treatment induces proliferation and inhibits apoptosis, while Iressa induces apoptosis.

  16. Verify Super Double-Heterogeneous Spherical Lattice Model for Equilibrium Fuel Cycle Analysis AND HTR Spherical Super Lattice Model for Equilibrium Fuel Cycle Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Gray S. Chang

    2005-11-01

    The currently being developed advanced High Temperature gas-cooled Reactors (HTR) is able to achieve a simplification of safety through reliance on innovative features and passive systems. One of the innovative features in these HTRs is reliance on ceramic-coated fuel particles to retain the fission products even under extreme accident conditions. Traditionally, the effect of the random fuel kernel distribution in the fuel pebble / block is addressed through the use of the Dancoff correction factor in the resonance treatment. However, the Dancoff correction factor is a function of burnup and fuel kernel packing factor, which requires that the Dancoff correction factor be updated during Equilibrium Fuel Cycle (EqFC) analysis. An advanced KbK-sph model and whole pebble super lattice model (PSLM), which can address and update the burnup dependent Dancoff effect during the EqFC analysis. The pebble homogeneous lattice model (HLM) is verified by the burnup characteristics with the double-heterogeneous KbK-sph lattice model results. This study summarizes and compares the KbK-sph lattice model and HLM burnup analyzed results. Finally, we discuss the Monte-Carlo coupling with a fuel depletion and buildup code - ORIGEN-2 as a fuel burnup analysis tool and its PSLM calculated results for the HTR EqFC burnup analysis.

  17. BMP-2, hypoxia, and COL1A1/HtrA1 siRNAs favor neo-cartilage hyaline matrix formation in chondrocytes.

    Science.gov (United States)

    Ollitrault, David; Legendre, Florence; Drougard, Carole; Briand, Mélanie; Benateau, Hervé; Goux, Didier; Chajra, Hanane; Poulain, Laurent; Hartmann, Daniel; Vivien, Denis; Shridhar, Vijayalakshmi; Baldi, Alfonso; Mallein-Gerin, Frédéric; Boumediene, Karim; Demoor, Magali; Galera, Philippe

    2015-02-01

    Osteoarthritis (OA) is an irreversible pathology that causes a decrease in articular cartilage thickness, leading finally to the complete degradation of the affected joint. The low spontaneous repair capacity of cartilage prevents any restoration of the joint surface, making OA a major public health issue. Here, we developed an innovative combination of treatment conditions to improve the human chondrocyte phenotype before autologous chondrocyte implantation. First, we seeded human dedifferentiated chondrocytes into a collagen sponge as a scaffold, cultured them in hypoxia in the presence of a bone morphogenetic protein (BMP), BMP-2, and transfected them with small interfering RNAs targeting two markers overexpressed in OA dedifferentiated chondrocytes, that is, type I collagen and/or HtrA1 serine protease. This strategy significantly decreased mRNA and protein expression of type I collagen and HtrA1, and led to an improvement in the chondrocyte phenotype index of differentiation. The effectiveness of our in vitro culture process was also demonstrated in the nude mouse model in vivo after subcutaneous implantation. We, thus, provide here a new protocol able to favor human hyaline chondrocyte phenotype in primarily dedifferentiated cells, both in vitro and in vivo. Our study also offers an innovative strategy for chondrocyte redifferentiation and opens new opportunities for developing therapeutic targets.

  18. Gender differences in the association between HTR2C gene variants and suicidal behavior in a Mexican population: a case–control study

    Directory of Open Access Journals (Sweden)

    Molina-Guzman G

    2017-02-01

    Full Text Available Gabriel Molina-Guzman,1 Thelma Beatriz González-Castro,2 Yazmín Hernández Díaz,2 Carlos Alfonso Tovilla-Zárate,3 Isela E Juárez-Rojop,3 Crystell Guadalupe Guzmán-Priego,3 Alma Genis,4 Sherezada Pool García,5 María Lilia López-Narvaez,6 José Manuel Rodriguez-Perez7 1División Académica de Ciencias de la Salud, Universidad Juárez Autónoma de Tabasco, Villahermosa, 2División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Jalpa de Méndez, 3División Académica Multidisciplinaria de Comalcalco, Universidad Juárez Autónoma de Tabasco, Comalcalco, Tabasco, México; 4Instituto Nacional de Medicina Genómica (INMEGEN, Servicios de Atención Psiquiátrica (SAP, Secretaría de Salud, Ciudad de México, México; 5Hospital General de Comalcalco, Secretaria de Salud, Comalcalco, Tabasco, México; 6Hospital General de Yajalón, Secretaría de Salud, Yajalón, Chiapas, México; 7Departamento de Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México Background: The aim of this case–control study was to explore the association by gender between the HTR2C gene variants and suicidal behavior in a Mexican population. Subjects and methods: A total of 183 suicide attempters and 208 healthy volunteers were included in this study. We genotyped five polymorphisms of HTR2C (rs547536, rs2192372, rs4272555, rs6318, and rs2428707, then measured the association by genotype, allele, and haplotype. Results: In the female group, we found an association between two polymorphisms of the HTR2C (rs4272555 and rs2428707 and suicide attempts. The C allele of the single-nucleotide polymorphism (SNP rs4272555 was associated with a decreased risk of suicide attempt (P=0.01, odds ratio =0.26, 95% confidence interval: 0.09–0.79, whereas the G allele of the SNP rs2428707 was associated with an increased risk of suicide attempt (P=0.01, odds ratio =3.68, 95% confidence interval: 1

  19. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORES 5, 6, 7, & 8: COLUMNAR HEXAGONAL POINT-ON-POINT PACKING WITH A 1:2 MODERATOR-TO-FUEL PEBBLE RATIO

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess

    2013-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  20. HTR-Proteus Pebble Bed Experimental Program Cores 5,6,7,&8: Columnar Hexagonal Point-on-Point Packing with a 1:2 Moderator-to-Fuel Pebble Ratio

    Energy Technology Data Exchange (ETDEWEB)

    Bess, John D. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sterbentz, James W. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Snoj, Luka [Idaho National Lab. (INL), Idaho Falls, ID (United States); Lengar, Igor [Idaho National Lab. (INL), Idaho Falls, ID (United States); Koberl, Oliver [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  1. Effect of Kaempferol on the BaP Induced Apoptosis in Human Chorionic Trophoblast Cells HTR8/SV neo%山奈酚对BaP引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    连立芬; 陈亚琼; 侯海燕; 曹波; 吴念

    2014-01-01

    目的:研究山奈酚对苯并芘(BaP)引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响。方法:通过四甲基偶氮唑盐(MTT)法观察及测定,最终选取10μmol/L BaP和0.01,0.1,1μmol/L山奈酚共同处理人HTR8/SV neo细胞48 h,光镜下观察不同浓度BaP作用下细胞生长情况,吉姆萨(Gimesa)染色及Hochest33342荧光染色观察细胞凋亡情况,利用流式细胞术测定细胞凋亡率变化。结果:①流式细胞术测定结果表明BaP单独作用细胞存活率为(72.58±0.29)%,与0.01,0.1,1μmol/L山奈酚同时作用时细胞存活率分别为(84.96±1.34)%、(89.54±1.64)%和(91.01±1.26)%,两者同时作用组细胞存活率均高于BaP单独作用组,差异均有统计学意义(P<0.01)。②单独BaP作用可引起细胞染色质浓缩,出现凋亡小体等典型凋亡形态、细胞核致密浓染、碎裂状。结论:山奈酚对BaP引起的HTR8/SV neo细胞凋亡有明显的抑制作用。%Objective:To study the effect of kaempferol on the BaP induced apoptosis in human chorionic trophoblast cells HTR8/SV neo. Methods:10μmol/L BaP were added 0.01,0.1,1μmol/L kaempferol jointly deal with human HTR8/SV neo cells 48 h,observed cells growth under the inverted microscope,Gimesa staining and Hochest33342 fluorescent staining observed morphologic changes,determination of apoptosis rate changes by flow cytometry. Results: ①Flow cytometry results showed that BaP alone cell viability was (72.58 ±0.29)%, with 0.01,0.1,1 μmol/L kaempferol the role of cell viability was (84.96 ±1.34)%, (89.54 ±1.64)% and (91.01 ±1.26)%, respectively, compared with BaP alone group, the differences were statistically significant (P<0.01). ②BaP alone can cause cell chromatin condensation,apoptotic bodies and other typical apoptotic morphology,dense hyperchromatic nuclei,fragmentation shape. Conclusions:Kaempferol showed a protective effect on BaP induced apoptosis in

  2. A putatively functional polymorphism in the HTR2C gene is associated with depressive symptoms in white females reporting significant life stress.

    Directory of Open Access Journals (Sweden)

    Beverly H Brummett

    Full Text Available Psychosocial stress is well known to be positively associated with subsequent depressive symptoms. Cortisol response to stress may be one of a number of biological mechanisms that links psychological stress to depressive symptoms, although the precise causal pathway remains unclear. Activity of the x-linked serotonin 5-HTR2C receptor has also been shown to be associated with depression and with clinical response to antidepressant medications. We recently demonstrated that variation in a single nucleotide polymorphism on the HTR2C gene, rs6318 (Ser23Cys, is associated with different cortisol release and short-term changes in affect in response to a series of stress tasks in the laboratory. Based on this observation, we decided to examine whether rs6318 might moderate the association between psychosocial stress and subsequent depressive symptoms. In the present study we use cross-sectional data from a large population-based sample of young adult White men (N = 2,366 and White women (N = 2,712 in the United States to test this moderation hypothesis. Specifically, we hypothesized that the association between self-reported stressful life events and depressive symptoms would be stronger among homozygous Ser23 C females and hemizygous Ser23 C males than among Cys23 G carriers. In separate within-sex analyses a genotype-by-life stress interaction was observed for women (p = .022 but not for men (p = .471. Homozygous Ser23 C women who reported high levels of life stress had depressive symptom scores that were about 0.3 standard deviations higher than female Cys23 G carriers with similarly high stress levels. In contrast, no appreciable difference in depressive symptoms was observed between genotypes at lower levels of stress. Our findings support prior work that suggests a functional SNP on the HTR2C gene may confer an increased risk for depressive symptoms in White women with a history of significant life stress.

  3. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORE 4: RANDOM PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess; Leland M. Montierth

    2013-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. One benchmark experiment was evaluated in this report: Core 4. Core 4 represents the only configuration with random pebble packing in the HTR-PROTEUS series of experiments, and has a moderator-to-fuel pebble ratio of 1:1. Three random configurations were performed. The initial configuration, Core 4.1, was rejected because the method for pebble loading, separate delivery tubes for the moderator and fuel pebbles, may not have been completely random; this core loading was rejected by the experimenters. Cores 4.2 and 4.3 were loaded using a single delivery tube, eliminating the possibility for systematic ordering effects. The second and third cores differed slightly in the quantity of pebbles loaded (40 each of moderator and fuel pebbles), stacked height of the pebbles in the core cavity (0.02 m), withdrawn distance of the stainless steel control rods (20 mm), and withdrawn distance of the autorod (30 mm). The 34 coolant channels in the upper axial reflector and the 33 coolant channels in the lower axial reflector were open. Additionally, the axial graphite fillers used in all other HTR-PROTEUS configurations to create a 12-sided core cavity were not used in the randomly packed cores. Instead, graphite fillers were placed on the cavity floor, creating a funnel-like base, to discourage ordering

  4. HTR-proteus pebble bed experimental program core 4: random packing with a 1:1 moderator-to-fuel pebble ratio

    Energy Technology Data Exchange (ETDEWEB)

    Bess, John D. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Montierth, Leland M. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sterbentz, James W. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Briggs, J. Blair [Idaho National Lab. (INL), Idaho Falls, ID (United States); Gougar, Hans D. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Snoj, Luka [Jozef Stefan Inst. (IJS), Ljubljana (Slovenia); Lengar, Igor [Jozef Stefan Inst. (IJS), Ljubljana (Slovenia); Koberl, Oliver [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    2014-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. One benchmark experiment was evaluated in this report: Core 4. Core 4 represents the only configuration with random pebble packing in the HTR-PROTEUS series of experiments, and has a moderator-to-fuel pebble ratio of 1:1. Three random configurations were performed. The initial configuration, Core 4.1, was rejected because the method for pebble loading, separate delivery tubes for the moderator and fuel pebbles, may not have been completely random; this core loading was rejected by the experimenters. Cores 4.2 and 4.3 were loaded using a single delivery tube, eliminating the possibility for systematic ordering effects. The second and third cores differed slightly in the quantity of pebbles loaded (40 each of moderator and fuel pebbles), stacked height of the pebbles in the core cavity (0.02 m), withdrawn distance of the stainless steel control rods (20 mm), and withdrawn distance of the autorod (30 mm). The 34 coolant channels in the upper axial reflector and the 33 coolant channels in the lower axial reflector were open. Additionally, the axial graphite fillers used in all other HTR-PROTEUS configurations to create a 12-sided core cavity were not used in the randomly packed cores. Instead, graphite fillers were placed on the cavity floor, creating a funnel-like base, to discourage ordering

  5. Recruitment by the Repressor Freud-1 of Histone Deacetylase-Brg1 Chromatin Remodeling Complexes to Strengthen HTR1A Gene Repression.

    Science.gov (United States)

    Souslova, Tatiana; Mirédin, Kim; Millar, Anne M; Albert, Paul R

    2016-12-02

    Five-prime repressor element under dual repression binding protein-1 (Freud-1)/CC2D1A is genetically linked to intellectual disability and implicated in neuronal development. Freud-1 represses the serotonin-1A (5-HT1A) receptor gene HTR1A by histone deacetylase (HDAC)-dependent or HDAC-independent mechanisms in 5-HT1A-negative (e.g., HEK-293) or 5-HT1A-expressing cells (SK-N-SH), respectively. To identify the underlying mechanisms, Freud-1-associated proteins were affinity-purified from HEK-293 nuclear extracts and members of the Brg1/SMARCCA chromatin remodeling and Sin3A-HDAC corepressor complexes were identified. Pull-down assays using recombinant proteins showed that Freud-1 interacts directly with the Brg1 carboxyl-terminal domain; interaction with Brg1 required the carboxyl-terminal of Freud-1. Freud-1 complexes in HEK-293 and SK-N-SH cells differed, with low levels of BAF170/SMARCC2 and BAF57/SMARCE1 in HEK-293 cells and low-undetectable BAF155/SMARCC1, Sin3A, and HDAC1/2 in SK-N-SH cells. Similarly, by quantitative chromatin immunoprecipitation, Brg1-BAF170/57 and Sin3A-HDAC complexes were observed at the HTR1A promoter in HEK-293 cells, whereas in SK-N-SH cells, Sin3A-HDAC proteins were not detected. Quantifying 5-HT1A receptor mRNA levels in cells treated with siRNA to Freud-1, Brg1, or both RNAs addressed the functional role of the Freud-1-Brg1 complex. In HEK-293 cells, 5-HT1A receptor mRNA levels were increased only when both Freud-1 and Brg1 were depleted, but in SK-N-SH cells, depletion of either protein upregulated 5-HT1A receptor RNA. Thus, recruitment by Freud-1 of Brg1, BAF155, and Sin3A-HDAC complexes appears to strengthen repression of the HTR1A gene to prevent its expression inappropriate cell types, while recruitment of the Brg1-BAF170/57 complex is permissive to 5-HT1A receptor expression. Alterations in Freud-1-Brg1 interactions in mutants associated with intellectual disability could impair gene repression leading to altered neuronal

  6. Association of 5-HTR2A gene-1438G/A polymorphisms with obsessive-compulsive disorder and efficacy of sertraline treatment%5-HTR2A基因-1438A/G多态性与强迫症及舍曲林疗效的关联研究

    Institute of Scientific and Technical Information of China (English)

    刘延辉; 张心华; 张立霞

    2013-01-01

    目的 探讨五羟色胺2A受体(5-HTR2A)基因-1438G/A多态性与强迫症(OCD)的关联及舍曲林疗效之间的关系.方法 随机抽取127例OCD患者作为研究对象,舍曲林(最高剂量200 mg/d)治疗8周,采用耶鲁-布朗强迫量表(Y-BOCS)评定疗效.应用聚合酶链反应-限制性片段长度多态性技术对OCD患者和健康对照(对照组,n=224)的5-HTR2A功能基因进行遗传关联分析.结果 5-HTR2A基因-1438G/A多态性位点的等位基因和基因型分布频率的分析结果显示:OCD组与对照组等位基因分布频率的差异有统计学意义(P<0.05),而基因型分布频率的差异无统计学意义(P>0.05);舍曲林治疗有效组与无效组等位基因分布频率的差异无统计学意义(P>0.05),而基因型分布频率的差异有统计学意义(P <0.05);OCD女性组等位基因和基因型分布频率与对照组女性比较,差异均有统计学意义(P <0.05);OCD早发组与对照组的基因型和等位基因分布频率的差异有统计学意义(P <0.05);OCD早发组与晚发组基因型和等位基因分布频率的差异均有统计学意义(P <0.05);OCD仅有强迫行为组与对照组的基因型和等位基因分布频率比较,差异均有统计学意义(P<0.05).结论 5-HTR2A基因-1438A等位基因可能是OCD患者发病的危险因素;-1438G/A多态性可能与OCD患者为女性、起病早、仅有强迫行为有关;舍曲林治疗OCD患者的疗效与5-HTR2A基因-1438G/A多态性的基因型存在关联.%Objective To investigate the association of 5-HT2A receptor (5-HTR2A) gene-1438G/A polymorphisms with obsessive-compulsive disorder (OCD) and efficacy of sertraline treatment.Methods One hundred and twenty-seven patients with OCD were selected,and were treated with sertraline for 8 weeks,with 200 mg/d as the highest dose of sertraline.The efficacy was rated by Yale-Brown Obsessive-Compulsive Scale (Y-BOCS).Polymerase chain reactionrestriction fragment length polymorphism

  7. Association between the HTR2C rs1414334 C/G gene polymorphism and the development of the metabolic syndrome in patients treated with atypical antipsychotics

    Directory of Open Access Journals (Sweden)

    José María Rico-Gomis

    2016-07-01

    Full Text Available Few studies have assessed the association between the rs1414334 C/G polymorphism in the HTR2C gene and the development of the metabolic syndrome in patients treated with atypical antipsychotics. To provide further evidence, a cross-sectional study was conducted in Spain between 2012 and 2013 in 166 patients with these characteristics. In these patients, the association between the polymorphism and the presence of the metabolic syndrome was determined by implementing binary logistic regression models adjusted for variables associated with the metabolic syndrome. We did not confirm previous claims that the C allele of the polymorphism was linked to the metabolic syndrome: the association was in the opposite direction and non-significant. This conclusion held after taking gender and lifestyle variables into account.

  8. HTR-PROTEUS Pebble Bed Experimental Program Cores 1, 1A, 2, and 3: Hexagonal Close Packing with a 1:2 Moderator-to-Fuel Pebble Ratio

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess; Barbara H. Dolphin; James W. Sterbentz; Luka Snoj; Igor Lengar; Oliver Köberl

    2013-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. Four benchmark experiments were evaluated in this report: Cores 1, 1A, 2, and 3. These core configurations represent the hexagonal close packing (HCP) configurations of the HTR-PROTEUS experiment with a moderator-to-fuel pebble ratio of 1:2. Core 1 represents the only configuration utilizing ZEBRA control rods. Cores 1A, 2, and 3 use withdrawable, hollow, stainless steel control rods. Cores 1 and 1A are similar except for the use of different control rods; Core 1A also has one less layer of pebbles (21 layers instead of 22). Core 2 retains the first 16 layers of pebbles from Cores 1 and 1A and has 16 layers of moderator pebbles stacked above the fueled layers. Core 3 retains the first 17 layers of pebbles but has polyethylene rods inserted between pebbles to simulate water ingress. The additional partial pebble layer (layer 18) for Core 3 was not included as it was used for core operations and not the reported critical configuration. Cores 1, 1A, 2, and 3 were determined to be acceptable benchmark experiments.

  9. HTR-PROTEUS Pebble Bed Experimental Program Cores 1, 1A, 2, and 3: Hexagonal Close Packing with a 1:2 Moderator-to-Fuel Pebble Ratio

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess; Barbara H. Dolphin; James W. Sterbentz; Luka Snoj; Igor Lengar; Oliver Köberl

    2012-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. Four benchmark experiments were evaluated in this report: Cores 1, 1A, 2, and 3. These core configurations represent the hexagonal close packing (HCP) configurations of the HTR-PROTEUS experiment with a moderator-to-fuel pebble ratio of 1:2. Core 1 represents the only configuration utilizing ZEBRA control rods. Cores 1A, 2, and 3 use withdrawable, hollow, stainless steel control rods. Cores 1 and 1A are similar except for the use of different control rods; Core 1A also has one less layer of pebbles (21 layers instead of 22). Core 2 retains the first 16 layers of pebbles from Cores 1 and 1A and has 16 layers of moderator pebbles stacked above the fueled layers. Core 3 retains the first 17 layers of pebbles but has polyethylene rods inserted between pebbles to simulate water ingress. The additional partial pebble layer (layer 18) for Core 3 was not included as it was used for core operations and not the reported critical configuration. Cores 1, 1A, 2, and 3 were determined to be acceptable benchmark experiments.

  10. Analysis of Random-Loading HTR-PROTEUS Cores with Continuous Energy Monte Carlo Code Based on A Statistical Geometry Model

    Energy Technology Data Exchange (ETDEWEB)

    Murata, Isao; Miyamaru, Hiroyuki [Division of Electrical, Electronic and Information Engineering, Osaka University, Yamada-oka 2-1, Suita, Osaka, 565-0871 (Japan)

    2008-07-01

    Spherical elements have remarkable features in various applications in the nuclear engineering field. In 1990's, by the project of HTR-PROTEUS at PSI various pebble bed reactor experiments were conducted including cores with a lot of spherical fuel elements loaded randomly. In this study, criticality experiments of the random-loading HTR-PROTEUS cores were analyzed by MCNP-BALL, which could deal with a random arrangement of spherical fuel elements exactly with a statistical geometry model. As a result of analysis, the calculated effective multiplication factors were in fairly good agreement with the measurements within about 0.5%DELTAk/k. In comparison with other numerical analysis, our effective multiplication factors were between the experimental values and the VSOP calculations. To investigate the discrepancy of the effective multiplication factors between the experiments and calculations, sensitivity analyses were performed. As the result, the sensitivity of impurity boron concentration was fairly large. The reason of the present slight overestimation was not made clear at present. However, the presently existing difference was thought to be related to the impurity boron concentration, not to the modelling of the reactor and the used nuclear data. From the present study, it was confirmed that MCNP-BALL would have an advantage to conventional transport codes by comparing with their numerical results and the experimental values. As for the criticality experiment of PROTEUS, we would conclude that the two cores of Core 4.2 and 4.3 could be regarded as an equivalent experiment of a reference critical core, which was packed in the packing fraction of RLP. (authors)

  11. Risk and protective genetic variants in suicidal behaviour: association with SLC1A2, SLC1A3, 5-HTR1B &NTRK2 polymorphisms.

    LENUS (Irish Health Repository)

    Murphy, Therese M

    2012-02-01

    BACKGROUND: Suicidal behaviour is known to aggregate in families. Patients with psychiatric disorders are at higher risk for suicide attempts (SA), however protective and risk genetic variants for suicide appear to be independent of underlying psychiatric disorders. Here we investigate genetic variants in genes important for neurobiological pathways linked to suicidal behaviour and\\/or associated endophenotypes, for association with SA among patients with co-existing psychiatric illness. Selected gene-gene and gene-environment interactions were also tested. METHODS: DNA was obtained from bloods of 159 patients (76 suicide attempters and 83 non-attempters), who were profiled for DSM-IV Axis I psychiatric diagnosis. Twenty-eight single nucleotide polymorphisms (SNPs) from 18 candidate genes (COMT, 5-HT2A, 5-HT1A, 5-HTR1B, TPH1, MAO-A, TPH2, DBH, CNR1, BDNF, ABCG1, GABRA5, GABRG2, GABRB2, SLC1A2, SLC1A3, NTRK2, CRHR1) were genotyped. Genotyping was performed by KBioscience. Tests of association between genetic variants and SA were conducted using Chi squared and Armitage Trend tests. Binary logistical regression analyses were performed to evaluate the contribution of individual genetic variants to the prediction of SA, and to examine SNPs for potential gene-gene and gene-environment interactions. RESULTS: Our analysis identified 4 SNPs (rs4755404, rs2269272, rs6296 and rs1659400), which showed evidence of association with SA compared to a non-attempter control group. We provide evidence of a 3-locus gene-gene interaction, and a putative gene-environment interaction, whereby genetic variation at the NTRK2 locus may moderate the risk associated with history of childhood abuse. CONCLUSION: Preliminary findings suggest that allelic variability in SLC1A2\\/3, 5-HTR1B and NTRK2 may be relevant to the underlying diathesis for suicidal acts.

  12. Nouvelles: KEK: B pour BELLE; NA48 mesure la violation directe de CP; LEL: le laser à électrons libres dépasse le kilowatt; Le LEP à 100 pour 100; Tour d'honneur au RHIC; Mesures de précision; Etude sur les modes de gestion du village planétaire; Conférence PANIC99 à Uppsala; Rencontre électron-noyau à HERA; LPP, Doubna célèbre une décennie; Les physiciens auscultent le photon; Aimants chauds venus du froid

    CERN Multimedia

    1999-01-01

    Nouvelles: KEK: B pour BELLE; NA48 mesure la violation directe de CP; LEL: le laser à électrons libres dépasse le kilowatt; Le LEP à 100 pour 100; Tour d'honneur au RHIC; Mesures de précision; Etude sur les modes de gestion du village planétaire; Conférence PANIC99 à Uppsala; Rencontre électron-noyau à HERA; LPP, Doubna célèbre une décennie; Les physiciens auscultent le photon; Aimants chauds venus du froid

  13. 五味子乙素通过NOS/NO系统预防BaP所致HTR8-SVneo细胞氧化损伤%Schizandrin B prevents benzo [a]-pyrene induced oxidative stress through NOS/NO system in HTR8-SVneo cells

    Institute of Scientific and Technical Information of China (English)

    章艳燕; 侯海燕; 陈晓; 梁婧; 陈亚琼

    2015-01-01

    目的 研究五味子乙素(Sch B)预防环境污染物苯并(a)芘(BaP)致人绒毛膜滋养层细胞HTR8-SVneo氧化损伤的机制. 方法 以HTR8-SVneo细胞为载体,构建BaP氧化应激模型.实验分为3组,BaP组、Sch B组和空白对照组.MTS法检测细胞存活率,还原酶法检测细胞培养液中一氧化氮(NO)及总一氧化氮合酶(TNOS)含量,用RT-PCR法检测细胞内诱导型一氧化氮合酶(iNOS)和内皮型一氧化氮合酶(eNOS)mRNA水平. 结果 BaP组细胞存活率(72.2±0.9)%较对照组显著下降(P<0.05);而0.1、0.5或2 μmol/L Sch B+20 μmol/L BaP各组的细胞存活率分别为(78.2±1.5)%、(86.5±0.6)%、(93.4±1.0)%,显著高于BaP组(P<0.05).BaP组细胞培养液中NO、TNOS含量、iNOS、eNOS mRNA表达量和eNOS蛋白表达量均显著高于对照组(P<0.05);而与BaP组相比,Sch B组中NO、TNOS含量,iNOS、eNOS mRNA表达量和eNOS蛋白表达量均随着Sch B剂量的增加逐渐下降(P<0.05). 结论 Sch B可有效预防BaP所致的HTR8-SVneo细胞氧化损伤,其机制可能与NO)S/NO系统的平衡有关.

  14. Xanthohumol impairs glucose uptake by a human first-trimester extravillous trophoblast cell line (HTR-8/SVneo cells) and impacts the process of placentation.

    Science.gov (United States)

    Correia-Branco, Ana; Azevedo, Cláudia F; Araújo, João R; Guimarães, João T; Faria, Ana; Keating, Elisa; Martel, Fátima

    2015-10-01

    In this study, we aimed to investigate modulation of glucose uptake by the HTR-8/SVneo human first-trimester extravillous trophoblast cell line by a series of compounds and to study its consequences upon cell proliferation, viability and migration. We observed that uptake of (3)H-deoxy-d-glucose ((3)H-DG; 10 nM) was time-dependent, saturable, inhibited by cytochalasin B (50 and 100 µM), phloretin (0.5 mM) and phloridzin (1 mM), insulin-insensitive and sodium-independent. In the short term (30 min), neither 5-HT (100-1000 µM), melatonin (10 nM) nor the drugs of abuse ethanol (100 mM), nicotine (100 µM), cocaine (25 µM), amphetamine (10-25 µM) and 3,4-methylenedioxy-N-methamphetamine (10 µM) affected (3)H-DG uptake, while dexamethasone (100-1000 µM), fluoxetine (100-300 µM), quercetin, epigallocatechin-3-gallate (30-1000 µM), xanthohumol (XH) and resveratrol (1-500 µM) decreased it. XH was the most potent inhibitor [IC50 = 3.55 (1.37-9.20) µM] of (3)H-DG uptake, behaving as a non-competitive inhibitor of (3)H-DG uptake, both after short- and long-term (24 h) treatment. The effect of XH (5 µM; 24 h) upon (3)H-DG uptake involved mammalian target of rapamycin, tyrosine kinases and c-Jun N-terminal kinases intracellular pathways. Moreover, XH appeared to decrease cellular uptake of lactate due to inhibition of the monocarboxylate transporter 1. Additionally, XH (24 h; 5 µM) decreased cell viability, proliferation, culture growth and migration. The effects of XH upon cell viability and culture growth, but not the antimigratory effect, were mimicked by low extracellular glucose conditions and reversed by high extracellular glucose conditions. We thus suggest that XH, by inhibiting glucose cellular uptake and impairing HTR-8/SVneo cell viability and proliferation, may have a deleterious impact in the process of placentation.

  15. Analysis of Boron Depletion in HTR Control Rod%高温气冷堆控制棒硼燃耗特性分析

    Institute of Scientific and Technical Information of China (English)

    赵晶; 李富; 刘志宏; 石秀安

    2011-01-01

    控制棒价值及其燃耗规律是核反应堆物理设计关注的要点之一.球床式高温气冷堆控制棒位于侧反射层石墨孔道中,吸收体为圆环形的B4C,其燃耗特性具有特殊性.采用MCNP耦合燃耗计算模块的方法,对控制棒吸收体进行精细划分,分析了各子区域硼的详细燃耗特性及控制棒价值的变化规律.计算结果表明,由于强烈的空间自屏效应,虽然吸收体外层硼燃耗很多,但吸收体内层硼燃耗很少,因此,反应堆运行寿期末控制棒价值减少很小.%The worth of control rod and its change along depletion are one of important features for reactor neutronics design. The control rods in pebble bed high-temperature gas-cooled reactor (HTR) were located in the hole of side graphite reflector. Its absorber was made up of annular B4C, which had special depletion rules. The depletion characteristics in each absorber sub-zone and the changes of the control rod worth were analyzed, based on both the MCNP coupling another depletion module and the detailed modeling control rod absorber region. According to the analysis, the boron in inner region of absorber is depleted only a little, although most of boron in outer region of absorber is depleted, because of the strong space self-shielding effects in control rod absorber. Therefore, the worth of control rod in the HTR decreases just a little at the end of reactor life time.

  16. Modelling of HTR (High Temperature Reactor Pebble-Bed 10 MW to Determine Criticality as A Variations of Enrichment and Radius of the Fuel (Kernel With the Monte Carlo Code MCNP4C

    Directory of Open Access Journals (Sweden)

    Hammam Oktajianto

    2014-12-01

    Full Text Available Gas-cooled nuclear reactor is a Generation IV reactor which has been receiving significant attention due to many desired characteristics such as inherent safety, modularity, relatively low cost, short construction period, and easy financing. High temperature reactor (HTR pebble-bed as one of type of gas-cooled reactor concept is getting attention. In HTR pebble-bed design, radius and enrichment of the fuel kernel are the key parameter that can be chosen freely to determine the desired value of criticality. This paper models HTR pebble-bed 10 MW and determines an effective of enrichment and radius of the fuel (Kernel to get criticality value of reactor. The TRISO particle coated fuel particle which was modelled explicitly and distributed in the fuelled region of the fuel pebbles using a Simple-Cubic (SC lattice. The pebble-bed balls and moderator balls distributed in the core zone using a Body-Centred Cubic lattice with assumption of a fresh fuel by the fuel enrichment was 7-17% at 1% range and the size of the fuel radius was 175-300 µm at 25 µm ranges. The geometrical model of the full reactor is obtained by using lattice and universe facilities provided by MCNP4C. The details of model are discussed with necessary simplifications. Criticality calculations were conducted by Monte Carlo transport code MCNP4C and continuous energy nuclear data library ENDF/B-VI. From calculation results can be concluded that an effective of enrichment and radius of fuel (Kernel to achieve a critical condition was the enrichment of 15-17% at a radius of 200 µm, the enrichment of 13-17% at a radius of 225 µm, the enrichments of 12-15% at radius of 250 µm, the enrichments of 11-14% at a radius of 275 µm and the enrichment of 10-13% at a radius of 300 µm, so that the effective of enrichments and radii of fuel (Kernel can be considered in the HTR 10 MW. Keywords—MCNP4C, HTR, enrichment, radius, criticality 

  17. A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling.

    Directory of Open Access Journals (Sweden)

    Juan Zhang

    Full Text Available Moderate invasion of trophoblast cells into endometrium is essential for the placental development and normal pregnancy. Electric field (EF-induced effects on cellular behaviors have been observed in many cell types. This study was to investigate the effect of physiological direct current EF (dc EF on cellular responses such as elongation, orientation and motility of trophoblast cells. Immortalized first trimester extravillous trophoblast cells (HTR-8/SVneo were exposed to the dc EF at physiological magnitude. Cell images were recorded and analyzed by image analyzer. Cell lysates were used to detect protein expression by Western blot. Cultured in the dc EFs the cells showed elongation, orientation and enhanced migration rate compared with non-EF stimulated cells at field strengths of 100 mV/mm to 200 mV/mm. EF exposure increased focal adhesion kinase (FAK phosphorylation in a time-dependent manner and increased expression levels of MMP-2. Pharmacological inhibition of FAK impaired the EF-induced responses including motility and abrogated the elevation of MMP-2 expression. However, the expression levels of integrins like integrin α1, α5, αV and β1 were not affected by EF stimulation. Our results demonstrate the importance of FAK activation in migration/motility of trophobalst cells driven by EFs. In addition, it raises the feasibility of using applied EFs to promote placentation through effects on trophoblast cells.

  18. Results of a Neutronic Simulation of HTR-Proteus Core 4.2 using PEBBED and other INL Reactor Physics Tools: FY-09 Report

    Energy Technology Data Exchange (ETDEWEB)

    Hans D. Gougar

    2009-08-01

    The Idaho National Laboratory’s deterministic neutronics analysis codes and methods were applied to the computation of the core multiplication factor of the HTR-Proteus pebble bed reactor critical facility. A combination of unit cell calculations (COMBINE-PEBDAN), 1-D discrete ordinates transport (SCAMP), and nodal diffusion calculations (PEBBED) were employed to yield keff and flux profiles. Preliminary results indicate that these tools, as currently configured and used, do not yield satisfactory estimates of keff. If control rods are not modeled, these methods can deliver much better agreement with experimental core eigenvalues which suggests that development efforts should focus on modeling control rod and other absorber regions. Under some assumptions and in 1D subcore analyses, diffusion theory agrees well with transport. This suggests that developments in specific areas can produce a viable core simulation approach. Some corrections have been identified and can be further developed, specifically: treatment of the upper void region, treatment of inter-pebble streaming, and explicit (multiscale) transport modeling of TRISO fuel particles as a first step in cross section generation. Until corrections are made that yield better agreement with experiment, conclusions from core design and burnup analyses should be regarded as qualitative and not benchmark quality.

  19. The HtrA-like serine protease PepD interacts with and modulates the Mycobacterium tuberculosis 35-kDa antigen outer envelope protein.

    Directory of Open Access Journals (Sweden)

    Mark J White

    Full Text Available Mycobacterium tuberculosis remains a significant global health concern largely due to its ability to persist for extended periods within the granuloma of the host. While residing within the granuloma, the tubercle bacilli are likely to be exposed to stress that can result in formation of aberrant proteins with altered structures. Bacteria encode stress responsive determinants such as proteases and chaperones to deal with misfolded or unfolded proteins. pepD encodes an HtrA-like serine protease and is thought to process proteins altered following exposure of M. tuberculosis to extra-cytoplasmic stress. PepD functions both as a protease and chaperone in vitro, and is required for aspects of M. tuberculosis virulence in vivo. pepD is directly regulated by the stress-responsive two-component signal transduction system MprAB and indirectly by extracytoplasmic function (ECF sigma factor SigE. Loss of PepD also impacts expression of other stress-responsive determinants in M. tuberculosis. To further understand the role of PepD in stress adaptation by M. tuberculosis, a proteomics approach was taken to identify binding proteins and possible substrates of this protein. Using subcellular fractionation, the cellular localization of wild-type and PepD variants was determined. Purified fractions as well as whole cell lysates from Mycobacterium smegmatis or M. tuberculosis strains expressing a catalytically compromised PepD variant were immunoprecipitated for PepD and subjected to LC-MS/MS analyses. Using this strategy, the 35-kDa antigen encoding a homolog of the PspA phage shock protein was identified as a predominant binding partner and substrate of PepD. We postulate that proteolytic cleavage of the 35-kDa antigen by PepD helps maintain cell wall homeostasis in Mycobacterium and regulates specific stress response pathways during periods of extracytoplasmic stress.

  20. Assessing the kinetics of high temperature oxidation of Inconel 617 in a dedicated HTR impure helium facility coupling thermogravimetry and gas phase chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Chapovaloff, J., E-mail: chpvlff@aol.com [AREVA NP, Centre Technique, Département Corrosion-Chimie, 30 Bd de l’industrie, 71200 Le Creusot (France); Ecole Nationale Supérieure des Mines, SMS-EMSE, CNRS: UMR5146, LCG, 158 Cours Fauriel, 42023 Saint Etienne (France); Rouillard, F., E-mail: fabien.rouillard@cea.fr [CEA, DEN, DPC, SCCME, Laboratoire d’Etude de la Corrosion Non Aqueuse, 91191 Gif sur Yvette (France); Combrade, P., E-mail: pierre.combrade@orange.fr [AREVA NP, Centre Technique, Département Corrosion-Chimie, 30 Bd de l’industrie, 71200 Le Creusot (France); ACXCOR, 63, chemin de l’Arnica, 42660 Le Bessat (France); Pijolat, M., E-mail: mpijolat@emse.fr [Ecole Nationale Supérieure des Mines, SPIN-EMSE, CNRS: UMR5148, LCG, 158 Cours Fauriel, 42023 Saint Etienne (France); Wolski, K., E-mail: wolski@emse.fr [Ecole Nationale Supérieure des Mines, SMS-EMSE, CNRS: UMR5146, LCG, 158 Cours Fauriel, 42023 Saint Etienne (France)

    2013-10-15

    Graphical abstract: Display Omitted -- Highlights: •New facility coupling thermogravimetry (TGA) with gas phase chromatography (GPC). •Dedicated for HT oxidation study in VHTR impure helium containing CO, H{sub 2}O and H{sub 2}. •The oxidation kinetics obeys a complete parabolic law due to a mixed kinetic regime. •CO contributes during initial stage of oxidation only for very low H{sub 2}O partial pressure. •Long-term oxidation of Inconel 617 by H{sub 2}O is diffusion controlled with constant kp. -- Abstract: A new facility coupling thermogravimetric analysis (TGA) with gas phase chromatography (GPC) has been developed. This facility is dedicated for studying high temperature oxidation of Inconel 617 in impure helium environment containing H{sub 2}O, H{sub 2} and CO at very low partial pressures (in the Pa range), which is representative of the high temperature reactor (HTR) concept developed within the Generation IV Forum. Simultaneous acquisition of mass gain and gas composition has allowed the influence of carbon monoxide and water vapour on the kinetics of oxidation to be studied. GPC measurements of gas consumption have allowed the plotting of individual mass gain curves for oxidation by H{sub 2}O and CO. During isothermal exposure at 1123 K for 20 h, the oxidation was mainly due to water vapour with a minor contribution of carbon monoxide during the first hours. The contribution of water vapour to the oxidation kinetics was extracted. It was shown to obey a complete parabolic law and to be limited by an interfacial reaction during the first few hours of oxidation and to be controlled by a mixed interfacial and diffusion process, diffusion becoming the rate-determining step for long term oxidation. There was very good agreement between GPC measurements and the experimental TGA results.

  1. Protective effect of nuclear factor E2-related factor 2 on inflammatory cytokine response to brominated diphenyl ether-47 in the HTR-8/SVneo human first trimester extravillous trophoblast cell line

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hae-Ryung, E-mail: heaven@umich.edu; Loch-Caruso, Rita

    2014-11-15

    Polybrominated diphenyl ethers (PBDEs) are widely used flame retardants, and BDE-47 is a prevalent PBDE congener detected in human tissues. Exposure to PBDEs has been linked to adverse pregnancy outcomes in humans. Although the underlying mechanisms of adverse birth outcomes are poorly understood, critical roles for oxidative stress and inflammation are implicated. The present study investigated antioxidant responses in a human extravillous trophoblast cell line, HTR-8/SVneo, and examined the role of nuclear factor E2-related factor 2 (Nrf2), an antioxidative transcription factor, in BDE-47-induced inflammatory responses in the cells. Treatment of HTR-8/SVneo cells with 5, 10, 15, and 20 μM BDE-47 for 24 h increased intracellular glutathione (GSH) levels compared to solvent control. Treatment of HTR-8/SVneo cells with 20 μM BDE-47 for 24 h induced the antioxidant response element (ARE) activity, indicating Nrf2 transactivation by BDE-47 treatment, and resulted in differential expression of redox-sensitive genes compared to solvent control. Pretreatment with tert-butyl hydroquinone (tBHQ) or sulforaphane, known Nrf2 inducers, reduced BDE-47-stimulated IL-6 release with increased ARE reporter activity, reduced nuclear factor kappa B (NF-κB) reporter activity, increased GSH production, and stimulated expression of antioxidant genes compared to non-Nrf2 inducer pretreated groups, suggesting that Nrf2 may play a protective role against BDE-47-mediated inflammatory responses in HTR-8/SVneo cells. These results suggest that Nrf2 activation significantly attenuated BDE-47-induced IL-6 release by augmentation of cellular antioxidative system via upregulation of Nrf2 signaling pathways, and that Nrf2 induction may be a potential therapeutic target to reduce adverse pregnancy outcomes associated with toxicant-induced oxidative stress and inflammation. - Highlights: • BDE-47 stimulated ARE reporter activity and GSH production. • BDE-47 resulted in differential

  2. Preliminary Study on Rotor Dynamics of Magnetic Bearing for 10MW High Temperature Gas-cooled Reactor%HTR-10磁轴承转子动力学的初步研究

    Institute of Scientific and Technical Information of China (English)

    杨国军; 于溯源

    2003-01-01

    采用有限元方法和MSC.Marc软件,分析了10MW高温气冷堆(HTR-10)能量转换系统磁轴承转子的模态,研究了磁轴承支承刚度与固有频率的关系.研究结果表明,可以通过改变支承刚度和转子材料来调整固有频率,为转子过两阶弯曲频率及磁轴承控制系统设计提供了依据.

  3. A Single Dose of LSD Does Not Alter Gene Expression of the Serotonin 2A Receptor Gene (HTR2A) or Early Growth Response Genes (EGR1-3) in Healthy Subjects

    Science.gov (United States)

    Dolder, Patrick C.; Grünblatt, Edna; Müller, Felix; Borgwardt, Stefan J.; Liechti, Matthias E.

    2017-01-01

    Rationale: Renewed interest has been seen in the use of lysergic acid diethylamide (LSD) in psychiatric research and practice. The repeated use of LSD leads to tolerance that is believed to result from serotonin (5-HT) 5-HT2A receptor downregulation. In rats, daily LSD administration for 4 days decreased frontal cortex 5-HT2A receptor binding. Additionally, a single dose of LSD acutely increased expression of the early growth response genes EGR1 and EGR2 in rat and mouse brains through 5-HT2A receptor stimulation. No human data on the effects of LSD on gene expression has been reported. Therefore, we investigated the effects of single-dose LSD administration on the expression of the 5-HT2A receptor gene (HTR2A) and EGR1-3 genes. Methods: mRNA expression levels were analyzed in whole blood as a peripheral biomarker in 15 healthy subjects before and 1.5 and 24 h after the administration of LSD (100 μg) and placebo in a randomized, double-blind, placebo-controlled, cross-over study. Results: LSD did not alter the expression of the HTR2A or EGR1-3 genes 1.5 and 24 h after administration compared with placebo. Conclusion: No changes were observed in the gene expression of LSD’s primary target receptor gene or genes that are implicated in its downstream effects. Remaining unclear is whether chronic LSD administration alters gene expression in humans. PMID:28701958

  4. 精神分裂症患者HTR2A基因多态性与帕利哌酮和利培酮临床疗效及安全性的关联研究%Association study of the HTR2A polymorphisms with efficacy and safety of risperidone or paliperidone in Chinese schizophrenic patients

    Institute of Scientific and Technical Information of China (English)

    杜波; 刘永桥; 刘飞虎; 肖卫东; 卢天兰; 李玲芝; 黄兰; 宓为峰; 张鸿燕

    2011-01-01

    目的 观察中国汉族精神分裂症患者的5-羟色胺2A受体(HTR2A)基因多态性与帕利哌酮和利培酮治疗精神分裂症患者临床疗效与安全性的关联.方法 入组201名精神分裂症患者,133例门诊或住院患者,随机接受12周帕利哌酮棕榈酸盐(50~150 mg/4周)或利培酮长效注射针剂(25~50 mg/4周)治疗,每2周进行1次疗效和安全性的评定;68例住院患者,接受4周利培酮片(2~6 mg·d-1)治疗,每周进行1次疗效和安全性评定;以试验结束时阳性阴性症状评定量表(PANSS)总减分率为主要疗效评价指标.共选择3个多态性位点,采用限制性酶切片段长度多态性或直接测序,获得基因型.结果 rs7997012各基因型可能与I临床疗效相关联(P<0.05),位点rs6313和rs6311的各基因型间临床疗效的差别无统计学意义(P>0.05);位点rs6313和rs6311的各基因型与BARS、SAS评分变化及血AST、ALT升高的水平相关联(P<0.05).结论 HTR2A 基因与帕利哌酮及利培酮治疗的临床疗效和安全性可能有关联.%Objective To study the relationship between gene polymorphisms of 5 - hydroxytryptamine receptor 2A (HTR2A) genes and the clinical efficacy and safety in Han Chinese schizophrenic patients who were treated with risperidone or paliperidone. Methods Two hundreds and one schizophrenic patients were included in this study, one hundred and thirty thee inpatients or outpatients were randomized to take paliperidone palmitate (50 - 150 mg/4 week) or risperidone LAI (25 -50 mg/2 week) for 12 weeks' therapy, efficacy and safety examines were taken at every visit. Sixty eight inpatients or outpatients who were treated with risperidone tablets (2 - 6 mg· d - 1 ) for 4 weeks were chosen. Efficacy and security examines was taken by every week. The main effect assessment was the final and baseline positive and negative syndrome scale(PANSS)total score reduced rate. The trial had chosen 3 SNPs, genotypes were detected by PCR

  5. 运用改良的CRISPR/Cas9系统建立HtrA2敲除细胞株%Generation of HtrA2 Knockout Cell Line with An Improved CRISPR/Cas9 System

    Institute of Scientific and Technical Information of China (English)

    胡袁; 张婷; 姜长安

    2014-01-01

    能识别特定基因组DNA序列的核酸酶是基因编辑的重要工具.在单链RNA引导下,来源于一种产脓链球菌的成簇规律间隔短回文重复(clustered regularly interspaced short palindromic repeats,CRISPR)关联蛋白(CRISPR-associated protein 9,Cas9)能够发挥其核酸酶功能对基因组特定序列进行剪切.这种功能依赖于单链引导RNA(single-guide RNAs,sgRNAs或gRNAs)中20 nt的核心靶向序列.在该研究中,作者对已有的CRISPR/Cas9系统载体进行了优化,简化了gRNA表达载体的构建.运用优化后的CRISPR/Cas9系统,我们敲除了HEK293T细胞中HtrA2基因的第一个外显子.这一改进大幅度降低了CRISPR/Cas9技术的使用成本.

  6. Plafonds froids et introduction d'air laminaire

    CERN Document Server

    Pepinster, P

    1998-01-01

    Dès le milieu des années 1980, on a pu observer une tendance à l'augmentation des charges thermiques internes dans les bâtiments administratifs, principalement due à l'arrivée de l'informatique et de la bureautique. Cet accroissement de charges a finalement atteint un record de 100 W/m2 fin des années 80 pour se stabiliser en règle générale entre 50 et 70 W/m2. Traditionnellement, c'est le renouvellement d'air refroidi et insufflé dans les locaux qui combat les apports calorifiques, et le débit d'air pulsé est proportionnel aux charges à évacuer. Cette augmentation des charges a donc généré des vitesses de circulation d'air résiduelles insatisfaisantes pour le confort des occupants, et les ingénieurs en climatisation ont dû revoir la technologie appliquée jusqu'alors. Ce document présente les techniques actuelles qui permettent de satisfaire à l'exigence d'absence de courant d'air aux places de travail, et ainsi assurer le bien-être humain dans le domaine climatique.

  7. Soldiers in Cold Environments (Soldats en environnements froids)

    Science.gov (United States)

    2009-04-01

    effectués sur un petit nombre de jeunes hommes en pleine forme et rarement sur des femmes ou des sujets plus âgés. Ceci n’est pas dû aux scientifiques...mais ce n’est pas le moindre, f) insister pour que des femmes et des sujets âgés de plus de 35 ans soient employés dans de futures expériences.

  8. Creation of a Full-Core HTR Benchmark with the Fort St. Vrain Initial Core and Assessment of Uncertainties in the FSV Fuel Composition and Geometry

    Energy Technology Data Exchange (ETDEWEB)

    Martin, William R.; Lee, John C.; baxter, Alan; Wemple, Chuck

    2012-03-31

    Information and measured data from the intial Fort St. Vrain (FSV) high temperature gas reactor core is used to develop a benchmark configuration to validate computational methods for analysis of a full-core, commercial HTR configuration. Large uncertainties in the geometry and composition data for the FSV fuel and core are identified, including: (1) the relative numbers of fuel particles for the four particle types, (2) the distribution of fuel kernel diameters for the four particle types, (3) the Th:U ratio in the initial FSV core, (4) and the buffer thickness for the fissile and fertile particles. Sensitivity studies were performed to assess each of these uncertainties. A number of methods were developed to assist in these studies, including: (1) the automation of MCNP5 input files for FSV using Python scripts, (2) a simple method to verify isotopic loadings in MCNP5 input files, (3) an automated procedure to conduct a coupled MCNP5-RELAP5 analysis for a full-core FSV configuration with thermal-hydraulic feedback, and (4) a methodology for sampling kernel diameters from arbitrary power law and Gaussian PDFs that preserved fuel loading and packing factor constraints. A reference FSV fuel configuration was developed based on having a single diameter kernel for each of the four particle types, preserving known uranium and thorium loadings and packing factor (58%). Three fuel models were developed, based on representing the fuel as a mixture of kernels with two diameters, four diameters, or a continuous range of diameters. The fuel particles were put into a fuel compact using either a lattice-bsed approach or a stochastic packing methodology from RPI, and simulated with MCNP5. The results of the sensitivity studies indicated that the uncertainties in the relative numbers and sizes of fissile and fertile kernels were not important nor were the distributions of kernel diameters within their diameter ranges. The uncertainty in the Th:U ratio in the intial FSV core was

  9. A candidate gene study of serotonergic pathway genes and pain relief during treatment with escitalopram in patients with neuropathic pain shows significant association to serotonin receptor2C (HTR2C)

    DEFF Research Database (Denmark)

    Brasch-Andersen, Charlotte; Møller, Malik U; Christiansen, Lene

    2011-01-01

    the association between polymorphisms in genes involved in the serotonergic pathway and the effect of escitalopram on peripheral neuropathic pain. METHODS: We genotyped 34 participants from a placebo-controlled trial of escitalopram in peripheral neuropathic pain for polymorphisms in five genes: the serotonin.......047), with 75% carrying the C allele being responders. The same tendency was seen in women. Similarly, carriership of the C allele at rs6318 was associated with better pain relief during treatment with escitalopram [odds ratio (OR) 15.5, p = 0.014)] Furthermore, there was a tendency of better relief...... with increasing number of short alleles for the 5-HTTLPR polymorphism of the serotonin transporter (OR 5.7, p = 0.057). None of the other polymorphisms showed a significant association with treatment response to escitalopram. CONCLUSION: This study indicates that variation in the HTR2C gene is associated...

  10. A genome-wide screen identifies Salmonella Enteritidis lipopolysaccharide biosynthesis and the HtrA heat shock protein as crucial factors involved in egg white persistence at chicken body temperature.

    Science.gov (United States)

    Raspoet, R; Shearer, N; Appia-Ayme, C; Haesebrouck, F; Ducatelle, R; Thompson, A; Van Immerseel, F

    2014-05-01

    Eggs contaminated with Salmonella Enteritidis are an important source of human foodborne Salmonella infections. Salmonella Enteritidis is able to contaminate egg white during formation of the egg within the chicken oviduct, and it has developed strategies to withstand the antimicrobial properties of egg white to survive in this hostile environment. The mechanisms involved in the persistence of Salmonella Enteritidis in egg white are likely to be complex. To address this issue, a microarray-based transposon library screen was performed to identify genes necessary for survival of Salmonella Enteritidis in egg white at chicken body temperature. The majority of identified genes belonged to the lipopolysaccharide biosynthesis pathway. Additionally, we provide evidence that the serine protease/heat shock protein (HtrA) appears essential for the survival of Salmonella Enteritidis in egg white at chicken body temperature.

  11. The association of 5-HTR2A-1438A/G, COMTVal158Met, MAOA-LPR, DATVNTR and 5-HTTVNTR gene polymorphisms and borderline personality disorder in female heroin-dependent Chinese subjects.

    Science.gov (United States)

    Yang, Mei; Mamy, Jules; Wang, Qiang; Liao, Yan-Hui; Seewoobudul, Vasish; Xiao, Shui-Yuan; Hao, Wei

    2014-04-03

    To explore the association between the 5-HTR2A-1438A/G, COMTVal158Met, MAOA-LPR, DATVNTR and 5-HTTVNTR polymorphisms with co-morbid borderline personality disorder (BPD) in female heroin-dependent patients. In a case control study, we compared the polymorphic distributions of 5-HTR2A-1438A/G, COMTVal158Met, MAOA-LPR, DATVNTR and 5-HTTVNTR in 296 female heroin-dependent patients (including 61 patients with BPD and 235 without BPD) and 101 normal females by genotypes, alleles, and interaction between genes. Female heroin-dependent subjects with BPD have lower frequency of the high activity allele (L: 4 repeats (4R)) of MAOA-LPR than those female heroin-dependent subjects without BPD, and have higher 5-HTTVNTR 10R/10R genotype frequency than normal female controls, with adjusted P-valuemultiple testing by 1000-fold permutation tests) respectively. By MDR (Multifactor Dimensionality Reduction) analyses, the interactive effects between MAOA-LPR and 5-HTTVNTR, and among MAOA-LPR, 5-HTTVNTR and rs6311 were close to the significance level (P=0.05) in predicting the risk of co-morbidity of BPD and heroin dependence relative to normal female controls, with 1000-fold permutation testing P-value0.05 respectively. 5-HTTVNTR and MAOA-LPR may have independent predictive effects on co-morbid BPD in female heroin-dependent patients; the gene-gene interactions between MAOA-LPR and 5-HTTVNTR, and among MAOA-LPR, 5-HTTVNTR and rs6311 might also be involved in the etiology of this co-morbidity. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Transient DNA damage induced by high-frequency electromagnetic fields (GSM 1.8 GHz) in the human trophoblast HTR-8/SVneo cell line evaluated with the alkaline comet assay.

    Science.gov (United States)

    Franzellitti, Silvia; Valbonesi, Paola; Ciancaglini, Nicola; Biondi, Carla; Contin, Andrea; Bersani, Ferdinando; Fabbri, Elena

    2010-01-05

    One of the most controversial issue regarding high-frequency electromagnetic fields (HF-EMF) is their putative capacity to affect DNA integrity. This is of particular concern due to the increasing use of HF-EMF in communication technologies, including mobile phones. Although epidemiological studies report no detrimental effects on human health, the possible disturbance generated by HF-EMF on cell physiology remains controversial. In addition, the question remains as to whether cells are able to compensate their potential effects. We have previously reported that a 1-h exposure to amplitude-modulated 1.8 GHz sinusoidal waves (GSM-217 Hz, SAR=2 W/kg) largely used in mobile telephony did not cause increased levels of primary DNA damage in human trophoblast HTR-8/SVneo cells. Nevertheless, further investigations on trophoblast cell responses after exposure to GSM signals of different types and durations were considered of interest. In the present work, HTR-8/SVneo cells were exposed for 4, 16 or 24h to 1.8 GHz continuous wave (CW) and different GSM signals, namely GSM-217 Hz and GSM-Talk (intermittent exposure: 5 min field on, 10 min field off). The alkaline comet assay was used to evaluate primary DNA damages and/or strand breaks due to uncompleted repair processes in HF-EMF exposed samples. The amplitude-modulated signals GSM-217 Hz and GSM-Talk induced a significant increase in comet parameters in trophoblast cells after 16 and 24h of exposure, while the un-modulated CW was ineffective. However, alterations were rapidly recovered and the DNA integrity of HF-EMF exposed cells was similar to that of sham-exposed cells within 2h of recovery in the absence irradiation. Our data suggest that HF-EMF with a carrier frequency and modulation scheme typical of the GSM signal may affect the DNA integrity.

  13. The design analysis for the main control room and standby shutdown point for HTR-PM%高温气冷堆核电站示范工程主控室和备用停堆点设计分析

    Institute of Scientific and Technical Information of China (English)

    冯静阁

    2011-01-01

    This paper describes the general design of the main control room(MCR)and standby shutdown point for high temperature gas-cooled reactor-pebble module(HTR-PM).The function of design,layout of control panels and main function and characteristics of MCR and standby shutdown point are specially introduced and analyzed in detail.%简要概述了华能山东石岛湾核电厂高温气冷堆核电站示范工程(简称HTR-PM)的主控室和备用停堆点的整体设计,着重对主控室和备用停堆点的功能设计、台盘布置、主要作用和特点进行了介绍和分析,为以后的实际工程提供参考。

  14. 高温气冷堆用石墨材料的腐蚀实验与软件模拟%Experiment and Simulation of Graphite Oxidation for HTR

    Institute of Scientific and Technical Information of China (English)

    魏明辉; 孙喜明

    2013-01-01

    In air or water ingress accident of the high temperature gas-cooled reactor (HTR) ,the corrosion of graphite components is inevitable result from oxygen and water steam ingression ,that may affect the reactor safety .Oxidation progress and the gas products of IG-110 nuclear graphite were studied using gas chromatograph .The numerical simulations were carried out by THERMIX/REACT software .The results show that temperature has a marked impact on IG-110 graphite oxidation .As well as the content proportion of CO to CO2 ,oxidation amount increases with temperature . The application of THERMIX/REACT for HTR safety analysis is validated by comparisons of the results of experimental and numerical simulation .%在高温气冷堆进水进空气事故下,空气和水蒸气会与堆内的石墨材料发生化学腐蚀反应,从而可能影响反应堆的安全。为研究高温气冷堆内石墨材料的氧化腐蚀特性,本文利用气相色谱法实验测量了IG-110石墨在不同温度和不同气体组分配比情况下的腐蚀速率及腐蚀产物,并利用THERMIX/REACT软件对整个石墨腐蚀过程进行了模拟。研究结果表明:反应温度对石墨腐蚀的影响最为显著,腐蚀速率随着温度的升高而增大,同时随着温度升高,CO与CO2的含量比也逐渐增大。通过与实验结果对比分析,验证了THERMIX/REACT软件用于高温气冷堆安全分析的可靠性。

  15. Calculation and Study on Model of Crushing Load of HTR Absorption Sphere%高温气冷堆吸收球压碎力模型的计算与研究

    Institute of Scientific and Technical Information of China (English)

    郭婷; 王晨; 董利民; 郭文利; 梁彤祥

    2013-01-01

    吸收球停堆系统是10 M W高温气冷堆的第二停堆系统。吸收球为含25% B4 C的石墨小球,B4 C弥散分布在石墨基体上。压碎力是吸收球的一重要性能指标,与球直径密切相关。采用石墨球模拟高温气冷堆吸收球,研究了石墨球直径和密度对压碎力的影响规律。选用3种不同密度的石墨,加工成5种不同直径的小球,进行压碎实验。研究结果表明:球的压碎力与直径的平方成正比;直径一定时,石墨球的密度越大,压碎力越大。提高石墨球的密度是提高压碎力的有效途径。%T he absorption sphere shutdow n system is the second shutdow n system of 10 MW HTR .The absorption sphere contains 25% B4C ,dispersing in graphite matrix . The crushing load ,which is an important performance parameter of the absorption sphere ,closely relates to the diameter . The effect of graphite sphere diameter and density on the crushing load was studied using the graphite sphere to simulate HTR absorption spheres .Three kinds of graphites with different densities were chosen and processed into five types of spheres with different diameters , and then the crushing experiment was conducted .The results show that the crushing load of the sphere is proportional to the square of the diameter ,and increases with the density .For a certain diameter of graphite sphere ,increasing the density of the sphere is an effective way to enhance the crushing load .

  16. 高温气冷堆示范电站一回路舱室区域模块化设计研究%Modular Design of Primary Cavity for HTR-PM Plant

    Institute of Scientific and Technical Information of China (English)

    王寿君; 齐中英

    2013-01-01

    The primary cavity is a key region and one of the difficult items to the con-struction of Pebble-bed Modular High Temperature Gas-cooled Reactor (HTR-PM ) demonstration plant .Modular design of primary cavity was studied in detail to enhance the construction quality and efficiency .The bracket modular design was adopted by the comparison of various design schemes .The modular division and design principles were proposed ,the modular design of this region was completed ,and a three-dimensional model for each module was built by using the three-dimensional design software CATIA .Analysis and calculation of strength and thermal stress of module support structure were completed .Construction feasibility and effect analysis show that the design scheme is feasible and the effect of reducing construction duration is obvious . The research has been applied in HTR-PM engineering design ,effectively increasing construction efficiency and significant economic benefits have been obtained .%一回路舱室是高温气冷堆示范电站建造的关键区域和难点之一。为提升该区域的建造质量和效率,针对一回路舱室区域开展模块化设计研究。通过方案比选,确定了支架模块方案的总体技术方案,提出模块化划分与设计的原则,完成该区域的模块化设计方案,利用三维设计软件CATIA构建各模块的三维模型,并对模块支承结构的强度和热应力进行设计校验,最后对设计方案的施工可行性与应用效果进行分析。结果表明,该方案可行,压缩工期效果明显。本研究结果在高温气冷堆示范电站工程上得到实际应用,有效提高了一回路舱室的建造效率,经济效益显著。

  17. 高温气冷堆氦气主流区中石墨粉尘运动特性初步分析%Preliminary Analysis of Graphite Dust Motion in Helium Mainstream for HTR

    Institute of Scientific and Technical Information of China (English)

    彭威; 杨小勇; 于溯源; 王捷

    2012-01-01

    Taking the graphite dust in helium mainstream in the primary loop of HTR as an example, the stresses acting on the single graphite dust in the helium mainstream is preliminarily calculated and analyzed. The result indicated that the gas drag played an absolute leading role. Based on the understanding of the importance of gas drag, a simplified motion equation of graphite dust was obtained, which was used to analyze the processes of graphite dust under various conditions. The results show that under a constant mainstream velocity, graphite dust particle velocity will approach the velocity of mainstream with the law of a negative exponent; while when the mainstream velocity changed linearly with time, the speed difference between graphite dust particle and mainstream will tend to a constant value; under the helium turbine cycle in HTR, the acceleration speed of graphite dust depended on the power.%以高温气冷堆一回路氦气中石墨粉尘颗粒为研究对象,初步计算分析氦气主流区中单个石墨粉尘颗粒的各种受力.结果表明,各种受力中,气体阻力占绝对主导作用.在分析受力的基础上,推导出一回路中石墨粉尘颗粒的简化运动控制方程,并计算分析石墨粉尘颗粒速度随氦气流速的运动规律:当主流氦气流速度恒定时,石墨粉尘颗粒速度按照负指数的运动规律不断趋近于主流氦气速度;当主流氦气速度随时间发生线性变化时,石墨粉尘颗粒速度与主流氦气速度趋向于一个恒定的差值;在高温气冷堆氦气气轮机循环中,石墨粉尘颗粒从静止到流动时的加速度变化与反应堆功率调节的增减呈现同一性.

  18. 5-HTR1A基因rs6295多态性与父母教养行为对青少年早期抑郁的交互作用:不同易感性模型的验证%The Interaction between rs6295 Polymorphism in the 5-HTR1A Gene and Parenting Behavior on Early Adolescents’ Depression:The Verification of Differential Susceptability Model

    Institute of Scientific and Technical Information of China (English)

    王美萍; 张文新; 陈欣银

    2015-01-01

    The extant findings showed 5-HTR1A gene rs6295 polymorphism was associated with depression. However, most of them were mainly guided by the “diathesis and stress model” and typically focused on the interaction between risk alleles and adverse environments. According to the “differential susceptibility model”, the individuals exclusively affected by negative contexts can also respond more favorably to positive environments, but the positive environments interacting with the same genes have scarcely been investigated. It also remains unclear whether there is a moderating effect of gender on the way rs6295 polymorphism interacting with environments. This study aimed to test the hypothesis of differential susceptibility model by examining the interaction of rs6295 polymorphism with positive and negative parenting behavior on early adolescent depression, and explore the mediating effect of adolescents’ gender. Participants (n= 1323) were a subset of a 4-year longitudinal study (n = 2715) which investigated 14 primary schools in Jinan by random cluster sampling method. During the initial assessment (in 2008), adolescents (grade 5) were on average 11.31 years old (SD = 0.49), and mothers ranged in age from 35 to 40 years (M = 38.03,SD = 2.39). Adolescents’ depression were identified via self-rating on the Children’s Depression Inventory (2008:α = 0.88; 2011:α = 0.89), and parenting behavior were rated by mother-report questionnaire (positive:α = 0.85; negative:α = 0.72). DNA was extracted from saliva. Genotype at rs6295 was performed in real time with MassARRAY RT software version 3.0.0.4 and analyzed using the MassARRAY Typer software version 3.4 (Sequenom). A series of linear regression analyses were conducted by SPSS 18.0. The results showed rs6295 polymorphism significantly interacted with positive parenting behaviors in predicting early adolescents’ depression, and furthermore this interaction was moderated by adolescents’ gender. Specifically

  19. Identification, expression, and pharmacology of a Cys{sub 23}-Ser{sub 23} substitution in the human 5-HT{sub 2C} receptor gene (HTR2C)

    Energy Technology Data Exchange (ETDEWEB)

    Lappalainen, J.; Ozaki, N.; Goldman, D. [National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (United States)] [and others

    1995-05-20

    The function of brain serotonin-2C (5-HT{sub 2C}) receptors, including behavioral and neurochemical responses to 5-HT{sub 2C} agonist challenge, has been suggested to be abnormal in individuals with neuropsychiatric disorders. Thus, it is important to identify polymorphisms and functional variants within this gene. Using SSCP analysis, the authors identified a Cys{sub 23}-Ser{sub 23} substitution (designated 5-HT{sub 2Ccys} and 5-HT{sub 2Cser}) in the first hydrophobic region of the human 5-HT{sub 2C} receptor. Allele frequencies in unrelated Caucasians were 0.13 and 0.87 for 5-HT{sub 2Cser} and 5-HT{sub 2Ccys}, respectively. DNAs from informative CEPH families were typed for this polymorphism and analyzed with respect to 20 linked markers on the X chromosome. Linkage analysis placed the 5-HT{sub 2C} receptor gene (HTR2C) on Xq24. To evaluate whether this amino acid substitution causes a variant function of this receptor, recombinant human 5-HT{sub 2Ccys} and 5-HT{sub 2Cser} receptors were expressed in Xenopus oocytes and tested for responses to 5-HT using electrophysiological techniques. Concentration-response curves for 5-HT were not significantly different in oocytes expressing either form of the receptor, suggesting that the 5-HT{sub 2Ccys} and 5-HT{sub 2Cser} receptor proteins may not differ in their responses to serotonin under baseline physiological conditions. 43 refs., 3 figs., 1 tab.

  20. Convertisseur de reequilibrage de la tension d'un reseau

    Science.gov (United States)

    Richardeau, Frédéric; Aouda, Noureddine; Meynard, Thierry

    1997-08-01

    This paper deals with the design, the control and the simulation of a voltage balancing static converter for a three phases utility distribution. Unbalancing sources and compensator structures are studied and compared. The balancing method consists in injecting the load currents negative and zero sequence in opposite phase by means of a PWM voltage source inverter. The identification of the load currents negative sequence is based on the fluctuating active and reactive power computing allowing a transient state compensation. A general formulation is proposed allowing to relate the unbalance parameters to the inverter design. The compensation currents and the DC voltage loops are studied and carried out. Presented simulation results allow to validate control loops and to refine the theoretical study on the basis of a specification. This study has been supported by a research contract between the LEEI and the CENTRALP Automatismes company. Cet article traite du dimensionnement, du pilotage et de la simulation d'un convertisseur statique de rééquilibrage dynamique de la tension d'un réseau de distribution. Les sources de déséquilibre sont identifiées et les structures statiques de rééquilibrage sont décrites. Le principe du rééquilibrage consiste en une compensation des systèmes inverse et homopolaire de courants au moyen d'un onduleur de tension en modulation de largeur d'impulsion. L'identification du système inverse de courants repose sur le calcul des puissances active et réactive fluctuantes mises en jeu par la charge déséquilibrée, permettant une compensatiori en régime transitoire. Une formulation générale du dimensionnement du compensateur est proposée permettant de lier les paramètres caractéristiques du déséquilibre à la valeur des éléments constitutifs. L'asservissement des courants et la régulation de la tension de l'onduleur sont analysés. Les résultats de simulation ont permis de valider et d'affiner l'étude théorique sur la base d'un cahier des charges. Cette étude s'inscrit dans le cadre d'une opération contractuelle entre le LEEI et les Etablissements CENTRALP Automatismes.

  1. AMETH laboratories network activities; Activites du reseau de Laboratoires AMETH

    Energy Technology Data Exchange (ETDEWEB)

    Marimbordes, T.; Ould El Moctar, A.; Peerhossaini, H. [Nantes Univ., Ecole Polytechnique, UMR CNRS 6607, Lab. de Thermocinetique, 44 (France)] [and others

    2000-07-01

    The AMETH laboratories are a network for the improvement of thermal exchanges for one or two phases. This meeting of the 15 november 2000, dealt with the activities of this network of laboratories in the following topics: thermal-hydrodynamic instabilities and control of the limit layer; transfers with change in the liquid-vapor phase; transfers with change in the solid-liquid phase. Ten papers were presented. (A.L.B.)

  2. The PACo channel strategy; La strategie du reseau PACo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    The PACo channel was created in june 1999 to answer government demands in the domain of new energies, to stimulate the new technologies development and to participate to enterprises growth. The first year was devoted in particular to the fuel cell and the hydrogen fuel. This document presents an analysis of the first year of program management. (A.L.B.)

  3. AMETH laboratories network activities; Activites du reseau de Laboratoires AMETH

    Energy Technology Data Exchange (ETDEWEB)

    Marimbordes, T.; Ould El Moctar, A.; Peerhossaini, H. [Nantes Univ., Ecole Polytechnique, UMR CNRS 6607, Lab. de Thermocinetique, 44 (France)] [and others

    2000-07-01

    The AMETH laboratories are a network for the improvement of thermal exchanges for one or two phases. This meeting of the 15 november 2000, dealt with the activities of this network of laboratories in the following topics: thermal-hydrodynamic instabilities and control of the limit layer; transfers with change in the liquid-vapor phase; transfers with change in the solid-liquid phase. Ten papers were presented. (A.L.B.)

  4. Technology channel fuel cells; Reseau technologique piles a combustible

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    This document presents the PACo channel, its research and development program and the calendar of the first year. The PACo channel aims at stimulate the technology innovation in the domain of the fuel cells and organize collaborations between enterprises and research laboratories. (A.L.B.)

  5. Dimensionnement-D-un-reseau-Sans-Fil-Wifi

    OpenAIRE

    BELABDELLI, Abdelheq; OUKAZ, Mokhtar

    2014-01-01

    Au cours de ce projet de fin d’études, nous avons étudié la technologie WIFI en présentant ses différentes architectures et nous avons développé un outil de dimensionnement d’un réseau de transmission de données haut débit d’accès WIFI. Lors de ce projet, nous avons présenté la technologie WIFI en montrant ces différentes fonctionnalités et en étudiant ses techniques d’accès au support. Ensuite, nous avons étudié les différentes architectures d’un réseau WI-FI : l’architecture en couche...

  6. Tri-generation in urban networks; Trigeneration en reseau urbain

    Energy Technology Data Exchange (ETDEWEB)

    Malahieude, J.M. [Trigen Energy Corp., New-York (United States)

    1996-12-31

    The concepts of tri-generation (simultaneous production of heat, electric power and refrigerating energy) and thermal energy distribution networks, are presented. The different components of the tri-generation system from Trigen Energy Corp. are ammonia as a refrigerant for the production of cooled water, screw compressors, gas turbines and an induction motor-generator in order to optimize the combined gas turbine and compressor utilization. The energy efficiency and pollution reduction of the system are evaluated; the system has been enhanced through re-powering and post combustion

  7. Error Analysis and Experimental Research of HTR-10 Burn-up Measurement System Based on MCNP Modeling%基于MCNP模型10MW高温气冷实验堆燃耗测量误差分析与实验研究

    Institute of Scientific and Technical Information of China (English)

    马涛; 夏冰; 王军令; 陈晓明; 江二东

    2012-01-01

    10MW高温气冷实验堆(HTR-10)的燃耗测量系统通过测量燃料球内裂变产物137Cs发出的γ射线进而间接确定燃料球的燃耗,测量结果的准确性直接影响着反应堆的安全性和经济性.利用HTR-10现有的设备条件,设计并实施了提升器偏转实验,使燃料球逐步偏离正常测量位,改变球心与准直器轴线的相对位置,得到了偏离角度与计数率之间的对应关系,进而确定燃料球球心与准直器轴线的周向偏移量.通过MCNP程序建立HTR-10燃耗测量系统模型,模拟γ光子从燃料球发出,经过提升器、密封法兰、准直器直到被HPGe晶体探测器捕捉的全过程.利用MCNP模型可以模拟在不同径向偏离情况下的实验过程,通过与实验结果的对比,确定燃料球球心偏离准直器轴线的径向偏移量.%With the burn-up measurement system fur the 10MW high temperature gas-rooletl reuctor. ihe hum-up of the spherical fuel element is obtained indirectly hy measuring the gamma-ray from the fission product 137Cs. The accuracy of results will affect the security and the economy of the reactor. In this paper, on the basis uf the existing equipment conditions of HTR-10, the diversion experiment of the elevator is designed and implemented. By making the spherical fuel element gradually deviate tiie running measurement place, the relative position between the center of the spherical fuel element and the axis of the rnllimalor is changed, and the relationship between the angle of she diversion and the count rale is obtained anri is then used lo determine the offset in the circumferential direction between the center of the spherical fuel element and the collimator axis. The model of the HTR-10 bum-up measurement system, established by the MCNP program, can he used to simulate the gamma photon transporting process from the spherical fuel element, through the elevator, the sealed flange and the collimator, to the HPCe detector. The MCNP model eon be

  8. 高温气冷堆石墨堆芯结构双层模型抗震研究%Seismic Study on Double-Layer Model of HTR Graphite Core Structure

    Institute of Scientific and Technical Information of China (English)

    孙立斌; 史力; 王洪涛; 王海涛; 张振声; 吴莘馨

    2012-01-01

    Graphite core structure contains pebble bed core, which is a key part of high temperature gas-cooled reactor. The dynamic response and structural integrality were studied under kinds of loads, especially earthquake, to meet the requirement of long term safe operation and sound design. The seismic test program on double-layer graphite core model, which is the part of HTR-PM side reflector structure, was carried out. Dynamic behavior including frequency response, acceleration, flexibility and bricks relative displacement were recorded. The keys and dowels stress, strain and destroy were analyzed. The important influencing factors were discussed by test and simulation results comparison. The experience obtained by the seismic test can improve the future more complicate graphite core structural seismic model research.%石墨堆芯结构包围球床堆芯,是高温气冷堆的重要组成部分.为满足其长期安全可靠运行的要求,使其设计合理、完整性得到保证,对石墨堆芯结构的动力学响应和结构完整性进行了研究,揭示其在运行中承受各种载荷条件(特别是地震)下的动态响应.完成的石墨堆芯结构双层模型抗震台试验中,测量模型在各种地震作用下的动力特性变化;考核石墨结构的刚度(变形)、强度和位移;分析榫、键等石墨构件的受力变形状况,整体结构的扭转.统计石墨构件经抗震试验后的破损数量,并分析了其破坏原因;通过初步的试验和模拟对比分析,探讨了石墨结构动力学响应的主要影响因素.为今后完成更大比例更复杂石墨堆芯结构模型抗震研究奠定了基础.

  9. Charges froides actives pour le calibrage des radiomètres micro ...

    African Journals Online (AJOL)

    In this paper, the state of the art of active cold loads is presented. These cold loads ... Keywords: Active cold load – Microwave radiometry – Calibration – Radiometers – Noise temperature ...... millimeter-wave solid state cold load, in Proc.

  10. Chaud show et froid à Libreville : consommation alimentaire et culture urbaine

    OpenAIRE

    Pourtier, R.

    1998-01-01

    Technological advances in food conservation have led to radical changes in eating habits in the capital of Gabon. A novel baking technique using microwave ovens has led to a revolution in bread production and distribution. As of 1987, fifty stands called "Hot Shows" (from the French term "Chaud Show") mushroomed throughout the city, selling crisp baguettes and pastries. Along with the more traditional-style bakeries, they have become new areas of urban life. Futhermore, the large proportion o...

  11. Recent evolutions of refrigerating machineries and heat pumps; Evolutions recentes des machines a froid et thermopompes

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-31

    This book of proceedings reports on 10 papers (or series of transparencies) concerning some recent developments about refrigerating machineries and heat pumps as used in space heating, air-conditioning and industrial refrigeration. Various aspects are developed: thermodynamic cycles, thermal performances, dimensioning, modeling, refrigerants substitution, design of flanged exchangers, compressors etc.. (J.S.)

  12. Scorching heat and energy: coldly analysis; Canicule et energie: analyse a froid

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-08-01

    Facing the scorching heat impacts of august 2003 in France, the author presents the necessity of an energy policy mastery: improvement of the thermal insulation of buildings to avoid the use of air conditioning, the automotive pollution abatement, safety of the electric network. (A.L.B.)

  13. Aspects électriques et thermiques de la fusion par induction en creuset froid

    OpenAIRE

    DELAGE, Daniel

    1982-01-01

    Induction is a method for heating electrically conductive materials and owning numerous advantages, mainly related to the ease and flexibility of use. It allows to focus on small volumes extremely high powers. So, heating times are very low. Moreover, depending on the choice of the frequency, the heated zones may be located in the bulk or at the surface only. This is a very reproducible heating system whose control is provided by adjusting the current in the inductor. The applications of indu...

  14. Studies and modeling of cold neutron sources; Etude et modelisation des sources froides de neutron

    Energy Technology Data Exchange (ETDEWEB)

    Campioni, G

    2004-11-15

    With the purpose of updating knowledge in the fields of cold neutron sources, the work of this thesis has been run according to the 3 following axes. First, the gathering of specific information forming the materials of this work. This set of knowledge covers the following fields: cold neutron, cross-sections for the different cold moderators, flux slowing down, different measurements of the cold flux and finally, issues in the thermal analysis of the problem. Secondly, the study and development of suitable computation tools. After an analysis of the problem, several tools have been planed, implemented and tested in the 3-dimensional radiation transport code Tripoli-4. In particular, a module of uncoupling, integrated in the official version of Tripoli-4, can perform Monte-Carlo parametric studies with a spare factor of Cpu time fetching 50 times. A module of coupling, simulating neutron guides, has also been developed and implemented in the Monte-Carlo code McStas. Thirdly, achieving a complete study for the validation of the installed calculation chain. These studies focus on 3 cold sources currently functioning: SP1 from Orphee reactor and 2 other sources (SFH and SFV) from the HFR at the Laue Langevin Institute. These studies give examples of problems and methods for the design of future cold sources.

  15. Basic physics with ultra cold neutrons; Physique fondamentale avec des neutrons ultra froids

    Energy Technology Data Exchange (ETDEWEB)

    Protasov, K. [Laboratoire de Physique Subatomique et de Cosmologie, CNRS-IN2P3, Universite Joseph Fourier, INPG, Grenoble (France)

    2007-07-01

    A short introduction to the physics of Ultra Cold Neutrons (UCN) is given. It covers different aspects from their discovery, their major properties as well as their using in the three experiments of fundamental physics: measurements of the neutron life time and of its electric dipole moment and studies of neutrons quantum states in the Earth's gravitational field. (author)

  16. The multi service cable network along a natural gas network for urban distribution; Reseau cable multiservice associe a un reseau de gaz naturel pour la consommation publique

    Energy Technology Data Exchange (ETDEWEB)

    Markovic, D.; Krsmanovic, Z. [NIS-Energogas (Yugoslavia)

    2000-07-01

    The paper discusses parallel construction and development of natural gas and telecommunications projects in Europe, with an emphasis on the situation in FR Yugoslavia. Deregulation of postal services has created an opportunity for joint construction of multipurpose cable networks and gas distribution systems. Advantages are shown of such joint construction of gas pipeline and cable systems, and the strategy of further development of telecommunications and gas supply projects in FR Yugoslavia is presented. (authors)

  17. 高温气冷堆示范工程反应堆保护系统故障树模型的建立和分析%Development and Analysis of Fault Tree Model of HTR-PM Reactor Protection System

    Institute of Scientific and Technical Information of China (English)

    郭超; 李铎; 熊华胜

    2013-01-01

    数字化保护系统正逐步取代模拟系统,应用于新建和升级的核电厂中,数字化保护系统的可靠性分析已成为仪控领域的热点研究课题。本工作以高温气冷堆示范工程(H T R-PM )的反应堆保护系统为研究对象,研究数字化保护系统故障树模型的建立和分析方法,主要研究内容包括:故障树顶事件的选取;基于失效模式与影响分析(FM EA )的故障树搭建方法,重点研究保护系统冗余通道的“2/4”表决逻辑以及通道旁通的处理方法;对故障树模型进行定性分析,并根据故障树的最小割集讨论保护系统的薄弱环节。该研究对于分析数字化保护系统的可靠性并改进系统设计具有重要意义。%Digital reactor protection system ( RPS ) is gradually replacing analog counterparts and being applied in newly-built and upgraded nuclear power plant (NPP) , and the analysis of digital RPS reliability is one of the hot topics in the research field of NPP I&C .Based on RPS of high temperature gas-cooled reactor pebble bed module (HTR-PM ) ,the research of this paper focused on development and analysis of digital RPS fault tree model ,which was outlined as following :The top event for a fault tree was chosen ;the method to build a fault tree model based on failure modes and effects analysis (FMEA) was studied ,specially focusing on the sub-tree of redundant channel“2-out-of-4” logic and the fault tree under one channel bypass ;the qualitative analysis of fault tree ,such as RPS weakness according to minimal cut sets was summarized .The research is important to analyze digital RPS reliability and to improve its design .

  18. B mesons phenomenology and lattice QCD; Phenomenologie des mesons B et chromodynamique quantique sur reseau

    Energy Technology Data Exchange (ETDEWEB)

    Blossier, B

    2006-06-15

    We have studied some phenomenological aspects of the B meson physics by using lattice QCD, which is a non perturbative method (based on the first principles of Quantum Field Theory) of computing Green functions of the theory. Pionic couplings g{sub 1} and g{sub 2}, parameterizing the effective chiral Lagrangian which describes interactions between heavy-light mesons and soft pions, have been computed beyond the quenched approximation (at N{sub f} = 2). We have renormalized the operator q-bar{gamma}{sub {mu}}{gamma}{sup 5}q non perturbatively by using chiral Ward identities. We obtain g{sub 1} = 0.4/0.6 and g{sub 2} = -0.1/-0.3. We have estimated from an un-quenched simulation (at N{sub f} = 2) the strange quark mass: the non perturbative renormalisation scheme RI-MOM has been applied. After the matching in the MS scheme the result is m{sub s}(2 GeV) = 101 {+-} 8(-0,+25) MeV. We have proposed a method to calculate on the lattice the Heavy Quark Effective Theory form factors of the semileptonic transitions B {yields} D{sup **} at zero recoil. The renormalisation constant of the operator h-bar{gamma}{sub i}{gamma}{sup 5}D{sub j}h has been computed at one-loop order of the perturbation theory. We obtain {tau}{sub 1/2}(1) = 0.3/0.5 and {tau}{sub 3/2}(1) 0.5/0.7. Eventually the bag parameter B{sub B{sub s}} associated the B{sub s} - B{sub s}-bar mixing amplitude in the Standard Model has been estimated in the quenched approximation by using for the strange quark an action which verifies the chiral symmetry at finite lattice spacing a. Thus systematic errors are significantly reduced in the renormalisation procedure because the spurious mixing of the four-fermion operator h-bar{gamma}{sub {mu}}{sub L}qh-bar{gamma}{sub {mu}}{sub L}q with four-fermion operators of different chirality is absent. The result is B{sub B{sub s}} = 0.92(3). (author)

  19. Activities of the Ameth laboratories network; Activites du reseau de laboratoires Ameth

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-31

    Thermal exchanges are of prime importance in industrial processes, space heating and air conditioning, cooling processes or heat exchangers design. University research laboratories and engineering schools created the Ameth network in order to share their means and competences in heat transfer research, to draw bridges between different subjects and to organize coordinated projects. In 1997 the Ameth laboratories have carried out 10 projects grouped into three themes: the thermal hydrodynamic instability and the limit boundary control, the transfers with liquid/vapor phase change and the transfers with solid/liquid phase changes. These projects and their first results are reported in this book of proceedings. This conference day was jointly organized by the thermal engineering university group (GUT) and the French society of thermal engineers. The ten communications presented in this book deal successively with: `the active control of a Poiseuille flow in the presence of volume heating`; `the control of instabilities, transition and thermo-convective flow transfers in cavities: application to the ventilation of rooms`; `the control of heat transfers during space-time flow disturbances in cylindrical channels`; `the characterization of surface heat transfers using transient methods improved for boiling in pool applications`; `the intensification of heat exchanges during boiling in confined medium`; `the evaporation and boiling in porous media`; `the characterization of heat transfers in micro-heat pipes`; `the AmETh 3-1 operation: experimental and numerical analysis of thermal resistances at the polymer-mould contact and developing during solidification`; `the analysis of solidification phenomena at the interface between a substrate and a melted medium`; and `the heat transfers with solid-liquid phase change`. (J.S.)

  20. ETUDE ET EVALUATION DES PERFORMANCES DU RESEAU WIFI MESH (802.11S)

    OpenAIRE

    BOUATIA, Wassila; MEZIANE TANI, Fadia Selma

    2014-01-01

    Dans notre étude, après avoir présenté les WMN, nous avons donné une description détaillées de plusieurs protocoles de routage suivi d'une étude comparative entre les protocoles AODV et DSR. Pour terminer nous avons exploité notre étude théorique avec des simulations sous NS-2, afin d'avoir le meilleur protocole en termes de QoS et proposé un modèle hybride entre les deux protocoles étudiés.

  1. Photovoltaic installation connected to the electric network; Installation photovoltaique raccordee au reseau

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2004-07-15

    This technical sheet on the connection of a photovoltaic installation to the electric network, provides information on the operating of such an installation, the possibilities of installation on a building, the possible subsidies, types of connection, environmental impacts, the electric power production, the cost estimation, the maintenance and life time and the administrative procedures. (A.L.B.)

  2. Dams on historic canal navigation networks; Les barrages sur le reseau des canaux historiques de navigation

    Energy Technology Data Exchange (ETDEWEB)

    Houde, L. [Parks Canada, Gatineau, PQ (Canada)

    2006-07-01

    This paper presented an overview of historic dams and other water retention structures within the Parks Canada network. It described the engineering achievement that went into the construction of these structures and how they have evolved over time. For the past century, the construction of canals has played a major role in Canada's economic development. The networks of canals have been used for transportation, commerce and military purposes and have acquired heritage and recreational value. Several canals, along with their locks and dams, have been recognized as having historical importance, such as the Chambly Canal in Quebec, and the Rideau Canal in Ottawa, Ontario. 14 refs.

  3. Construction et maintien d'une for\\^et couvrante dans un r\\'eseau dynamique

    CERN Document Server

    Pigné, Yoann; Guinand, Frédéric; Chaumette, Serge

    2010-01-01

    In this work we introduce the principles of an algorithm that constructs and maintains a spanning forest in a mobile telecommunication network-a MANET. The algorithm is based on the random walk of a token and is entirely decentralized. A probability analysis is performed when the network is static. Then we show that performances can be slightly enhanced when adding a memory process in the walk on the token.

  4. Radiochemical aging of an epoxy network; Vieillissement radiochimique d'un reseau epoxyde

    Energy Technology Data Exchange (ETDEWEB)

    Devanne, Th

    2003-05-01

    This thesis is to give a better understanding of the radiochemical aging of a thermoset resin under gamma irradiation. The conditions of aging are gamma irradiation under air with a dose rate of 2 kGy/h at 120 C. The requested lifetime is four years, it means a dose of 70 MGy. The first step of this work was the choice of a resistive epoxy resin. This choice was made thanks to the literature data. The high glass transition temperature and the high amount of aromatic groups were the main criteria of the final choice. After this choice, thermal and mechanical properties were followed under thermal and radiochemical aging: i) under thermal aging, after 600 hours at 220 C, the glass transition temperature remained unchanged. But, from a mechanical point of view, properties at break dramatically decreased. This embrittlement was assigned to a critical oxidized layer. The thickness of this layer was estimated about 30 {mu}m. ii) the same kind of embrittlement was observed under radiochemical aging. Moreover, it appeared a decrease of the glass transition temperature when increasing the dose of irradiation. This indicates that the main degradation mechanism is chain scission under anaerobic atmosphere. We, then, proposed a mechanistic model associated with a kinetic model to predict the evolution of the glass transition temperature depending on the irradiation conditions. Parameters of the kinetic model were determined by solid NMR and ESR experiments. Comparison between experimental and calculated values at 120 C is satisfactory, a global good agreement was found. (author)

  5. District cool water distribution; Reseau urbain et distribution d`eau glacee

    Energy Technology Data Exchange (ETDEWEB)

    Schabaillie, D. [Ste Climespace (France)

    1997-12-31

    The city of Paris has developed several district cool water distribution networks (Climespace) for air conditioning purposes, one in the Halles district (central Paris) linked with the Louvre museum, one in the Opera district (with large department stores) and one in the east of paris (Bercy). Each of these networks has a cool water production plant, the one at the Halles producing also hot water and safety electric power. The characteristics of the equipment (heat pumps, refrigerating machinery, storage...) are described. The pipes are laid in the city sewage network, and the cool carrier is water. The various networks are centrally supervised at the Halles center

  6. Influence des caracteristiques geomecaniques du reseau de fractures sur les pressions d'injection limites

    Science.gov (United States)

    Lim, Sophie

    Grouting jointed rock masses improves their water tightness and strength for civil infrastructure foundations such as hydroelectric dams. In North America, the most common method involves injecting grout in fractures at a pressure given by a rule of thumb of 25 kPa per meter of rock cover. The criterion used to define the limit pressure is conservative because it considers a state of stress due to gravity only and does not take into account the geomechanical characteristics of the rock mass and the properties of the injection grout. The objective of this study is to verify the influence of geomechanical parameters on maximum injection pressures and compare it to the empirical criterion of 25 kPa /m using UDEC, a distinct element code for 2D modelling of fractured rock masses. The hydrojacking mechanism during fracture injection is sensitive not only to the grout pressure but also to its rheological parameters as well as the strength and the opening of the fractures. In this study, grouting in different rock mass models is simulated with conditions (constant-width fractures openings and horizontal stress equivalent to the vertical stress) favorable to hydrojacking. With the numerical model, hydrojacking was observed at injection pressures slightly higher than those prescribed by the industry. The jacking mechanism is mainly related to fractures near the surface. Because the model does not include the possibility of introducing time dependent grout parameters, grout flows at larger distances than what is observed in the field. In addition, the two-dimensional model has certain limitations and requires assumptions restricting the direct transposition of results to the field due to the complexity of fracture geometries and the grout spreading in 3D. A relative comparison of limit grouting pressures is nonetheless possible and it highlights the effects of certain geomechanical parameters on its magnitude.

  7. Optical lattice clock with Strontium atoms; Horloge a reseau optique a atomes de strontium

    Energy Technology Data Exchange (ETDEWEB)

    Baillard, X

    2008-01-15

    This thesis presents the latest achievements regarding the optical lattice clock with Strontium atoms developed at LNE-SYRTE. After a review of the different types of optical clocks that are currently under development, we stress on the concept of optical lattice clock which was first imagined for Sr{sup 87} using the {sup 1}S{sub 0} {yields} {sup 3}P{sub 0} transition. We exhibit the features of this atom, in particular the concept of magic wavelength for the trap, and the achievable performances for this kind of clock. The second part presents the experimental aspects, insisting particularly on the ultra-stable laser used for the interrogation of the atoms which is a central part of the experiment. Among the latest improvements, an optical pumping phase and an interrogation phase using a magnetic field have been added in order to refine the evaluation of the Zeeman effect. Finally, the last part presents the experimental results. The last evaluation of the clock using Sr{sup 87} atoms allowed us to reach a frequency accuracy of 2.6*10{sup -15} and a measurement in agreement with the one made at JILA (Tokyo university) at the 10{sup -15} level. On another hand, thanks to recent theoretical proposals, we made a measurement using the bosonic isotope Sr{sup 88} by adapting the experimental setup. This measurement represents the first evaluation for this type of clock, with a frequency accuracy of 7*10{sup -14}. (author)

  8. Transport Corrections in Nodal Diffusion Codes for HTR Modeling

    Energy Technology Data Exchange (ETDEWEB)

    Abderrafi M. Ougouag; Frederick N. Gleicher

    2010-08-01

    The cores and reflectors of High Temperature Reactors (HTRs) of the Next Generation Nuclear Plant (NGNP) type are dominantly diffusive media from the point of view of behavior of the neutrons and their migration between the various structures of the reactor. This means that neutron diffusion theory is sufficient for modeling most features of such reactors and transport theory may not be needed for most applications. Of course, the above statement assumes the availability of homogenized diffusion theory data. The statement is true for most situations but not all. Two features of NGNP-type HTRs require that the diffusion theory-based solution be corrected for local transport effects. These two cases are the treatment of burnable poisons (BP) in the case of the prismatic block reactors and, for both pebble bed reactor (PBR) and prismatic block reactor (PMR) designs, that of control rods (CR) embedded in non-multiplying regions near the interface between fueled zones and said non-multiplying zones. The need for transport correction arises because diffusion theory-based solutions appear not to provide sufficient fidelity in these situations.

  9. MINERVA, qualification of dissimilar welds for HTR-application

    Energy Technology Data Exchange (ETDEWEB)

    Baumann, B.; Gnirss, G.; Neureuther, H.; Schneider, K.

    1989-03-01

    The component test ''MINERVA'' is part of an overall concept to qualify dissimilar welds in pipings of advanced gas-cooled high temperature reactors. ''MINERVA'' is designed for a testing time of 20000 h. The experiment started in January 1987. The paper describes the concept, the manufacuring parameters including the basis of selection of materials and welding procedures. The experiment MINERVA and the operating conditions are described.

  10. Development of the fuel HTR/VHTR at the CEA; Developpement du combustible HTR/VHTR au CEA

    Energy Technology Data Exchange (ETDEWEB)

    Phelip, M.; Charollais, F.; Lambert, Th

    2005-07-01

    This presentation brings together the slides presented during the conference of the SESC/SPUA the 28 January 2005. The first part is devoted to the fuel behavior under irradiation, the second one to the main objectives of the program and the last part to the international collaboration. It provides information on the fuel behavior under irradiation, the fuel fabrication and performance, the SIROCCO program, the european contacts and the simulation programs. (A.L.B.)

  11. The Design of HTR-10 Control Rod Damper%HTR-10控制棒减震器设计

    Institute of Scientific and Technical Information of China (English)

    袁碧; 周惠忠; 薛松

    2002-01-01

    通过对高温气冷堆控制棒减震器结构设计,有限元模拟与计算,分析焊缝与构件的几何尺寸对减震效果的影响,最后通过实验验证,证明了理论计算与结构设计的合理性.

  12. Quelques problèmes à petit nombre d'atomes froids dans des guides d'onde.

    OpenAIRE

    2015-01-01

    This manuscript is motivated by the possibility of exploring many quantum regimes using cold atoms: interactions can be tuned very precisely by using Feshbach resonances and low-dimensional regimes can be approached with external potentials. Here, we study some properties of cold atoms trapped into uni- or bidimensional waveguides in the vicinity of a Feshbach resonance. The transition from a tridimensionnal to a low-dimensional system can then be studied by varying the intensity of the confi...

  13. Organiser à froid les règles de la liquidité entre banques et banque centrale

    OpenAIRE

    Dominique Hoenn

    2011-01-01

    The liquidity crisis that occurred in 2008 brought the world banking system near collapse. Therefore central banks had to step urgently and massively in the money market in order to supply banks with liquidities and they were constrained to buy assets of dubious quality. Dominique Hoenn considers that the ongoing reforms of the banking system will not be sufficient to prevent the occurrence of another financial crisis with its economic consequences. To avoid such a situation, he recommends th...

  14. Amelioration de l'adhesion de revetements organiques deposes par plasma froid sur polymeres pour applications biomedicales

    Science.gov (United States)

    Sbai, Marouan

    Plasma surface modification is commonly used in biomedical field, for example to enhance cell adhesion and growth surrounding the stent covers without affecting its bulk properties. Plasma polymer (PP) deposition used to create thin films rich in functional groups, e.g. primary amines, known to enhance the cellular response and allow grafting of biomolecules especially on stent grafts. Thin film adhesion to stent polymeric cover should be considered especially as they will evolve in a biological environment. The aim of this project is to evaluate the adhesion of PP on polytetrafluoroethylene (PTFE) and polyethyleneterephthalate (PET). Thereafter, an ammonia plasma treatment on PTFE is performed prior to deposition of PP to optimize the PP/PTFE adhesion. PP studied here (referred to as "LP") is prepared from a mixture of ethylene (C2H4) and ammonia (NH3). It is deposited on two supports, PET and PTFE. The interfacial adhesion between the LP coating and the substrate was evaluated by "Peel-test 180 °" according to ASTM F1842. Staining of the surface after peel test followed by an image analysis was performed to determine the percentage of removed coating. Adhesion optimization is done by varying operating plasma parameters such as power, pressure and pretreatment time. Chemical analyses and wettability of LP and pretreated surfaces in dry and wet conditions are characterized by XPS and contact angle measurements, respectively. The adhesion of LP/PET was excellent in a dry environment (water, respectively). However, 56% to 75% of the LP is removed from virgin PTFE in a dry and wet environment, respectively; percentages can be substantially reduced by plasma pretreatment (0% and 8+/-3% in air and 30min in deionized water). Almost no delamination was observed with NH3 plasma pretreatment at 15s, 100 mTorr and 50W. N2 plasma pretreatment, for comparison, proves much less effective. The LP/PTFE adhesion is considerably improved by plasma pretreatment compared to untreated samples. The chemical LP composition and the cell growth on LP/pretreated PTFE are not significantly affected by this pretreatment. Further investigations are required to explain the LP/PET low adhesion in wet conditions.

  15. Les dérangements du temps. 500 ans de chaud et de froid en Europe, Emmanuel GARNIER, 2010

    Directory of Open Access Journals (Sweden)

    Jean-Louis Ballais

    2011-12-01

    Full Text Available Après un avant-propos : Il y a encore de la lumière !, l'ouvrage s'organise en six parties.La première partie (p. 19-48 : Vous avez dit histoire du climat ?, présente à la fois la position de l'auteur dans le débat sur les causes du réchauffement climatique et les méthodes qu'il a utilisées. Emmanuel GARNIER se positionne clairement sur les positions du GIEC, non sans nuances et non sans simplifier les positions des sceptiques sur le rôle des gaz à effet de serre d'origine anthropique. Il re...

  16. Regulation in climate control engineering. Refrigeration, air conditioning, space heating; Regulation en genie climatique. Froid, climatisation, chauffage

    Energy Technology Data Exchange (ETDEWEB)

    Desmons, J.

    2005-04-01

    After a recall of some basic concepts of electronics and hydraulics, this book presents some methodological and technological elements about regulation in climate control engineering. It explains how to design and implement regulation systems and how to practice their maintenance. It presents several practical cases of real installations. (J.S.)

  17. Caracterisation des contraintes residuelles engendrees par l'expansion a froid de trous dans des alliages d'aluminium

    Science.gov (United States)

    Lapalme, Maxime

    Cold Expansion (CX) is a process which consists in plastically deforming assembly holes in metallic alloys by drawing an oversize mandrel through them. The major interference caused by the mandrel generates residual constraints around the hole. The tangential part of those constraints is beneficial for the hole fatigue life since an highly compressive zone is created which will retard fatigue cracks propagation. However, farther from this compressive zone, balancing tensile stresses are generated. The resultant of the CX process is a considerable increase in the fatigue life of the hole which has been demonstrated by the industry over the last decades. The present study objectives were the characterization of the residual stress field induced by CX and the development of a simulation method for it. The complexity of the generated stresses is increased tenfold by two main elements. First, the progressive drawing of the mandrel through the hole causes a scalable interference which produces variable stress states in the thickness of the perforated plate. Second, for easier application and productivity, the interference between the hole and the mandrel is actually caused by an interference object, the sleeve, that is rolled to a cylindrical form from a thin steel sheet. At its critical interference position, a split is opened in the sleeve which causes a non-uniform mechanical loading applied to the walls of the hole. In order to conceive a physically realistic tridimensional finite element model, laboratory measurements were first performed. The mandrel was digitized to introduce its exact shape in the model. Dimensional measurements have also helped to characterize the sleeve mechanical behavior during the CX and the effect of its split on the final hole state. These measurements and observations allowed defining the behavior of various interfaces of contact and geometries in the FE model. Characterization of the residual stress field and the validation of the simulation model of CX were performed using a variety of experimental data generated as part of this study. First, X-ray diffraction yielded measurements of stress on both sides of the sample. Then, full field planar strains were measured using digital image correlation on both sides of samples. Finally, optical measurements were carried out to determine the out-of-plane displacements at the vicinity of the hole, movement which is caused by the passage of the mandrel and the flow of material as it moves. The experimental data showed that through the thickness of a plate with a hardened hole, the residual stresses and strains are quite different, and therefore that the CX process has important three-dimensional effects. Moreover, the opening in the sleeve causes a state of nonuniform deformation on the circumference of the hole. The results of the simulation using the developed FE model show a very good correlation with the experimental data gathered for stress, strain and displacement. This comparison shows that to properly simulate the CX process, it is important to consider the exact geometry of the parts and tools as well as contacts between all of these interfaces. Following CX, the hole is generally reamed to the dimensions required for the subsequent assembly with a fastener. This machining causes a redistribution of the stress previously generated by CX. No experimental results have been collected on the impact of the reaming in the context of this study. However, a simulation method was used in the FE model to represent this last operation. The analysis shows that the reaming uniforms stress state across the thickness of the hardened sample. A validation of this observation would be necessary since the effect is significant on the final condition of the residual stresses generated by CX.

  18. Coldness generation and heat revalorization: cryogenic machines; Production de froid et revalorisation de la chaleur: machines cryogeniques

    Energy Technology Data Exchange (ETDEWEB)

    Feidt, M. [Institut National des Sciences Appliquees (INSA), 69 - Villeurbanne (France)

    2005-12-01

    This study treats more particularly of the generation and use of very low temperatures (typically below -100 deg. C). Such temperatures involve different techniques and new physical principles which are examined in this document. The high temperature re-valorization of heat remains poorly explored and is just evoked in this document. Content: 1 - temperature range of cryogenics; 2 - cascade cycles; 3 - gases liquefaction: liquid air, liquid helium, particular properties of helium and refrigeration (Pomaranchuk effect, helium refrigerators); 4 - thermomagnetic effects: basic principles, magnetic refrigerating machine; 5 - conclusions and perspectives about cryogenics. (J.S.)

  19. Formation of cold molecules through the photo-association of cold atoms of Cesium. Existence of long range forces between between cold excited atoms of Cesium; Formation de molecules froides par photoassociation d'atomes froids de cesium. Mise en evidence de forces a longue portee entre atomes froids excites de cesium

    Energy Technology Data Exchange (ETDEWEB)

    Comparat, D

    1999-09-01

    This thesis deals with the experimental study and the theoretical interpretation of the processes involved in photo-association and the formation of cold caesium molecules. It also presents a study of the dipolar forces between a pair of cold excited caesium atoms. We present here the first photo-association experiment on cold caesium atoms: two cold atoms absorb a photon to form an excited electronically excited molecules in a rotation-vibration level. The first production of cold molecules which was realised experimentally, after the spontaneous deexcitation of the photo-associated molecules, is described, stressing the role of the potential well of the molecular states O{sub g}{sup -}(6s+6p{sub 3/2}) or 1{sub u} (6s+6p{sub 3/2}) of caesium. The detection of the formed caesium molecules is based on a two-photons resonant ionisation that creates Cs{sub 2}{sup +} ions, afterwards selectively detected. Temperatures around 20-200 {mu}K have been measured. The photo-associative spectroscopy is described on the theoretical point of view: a detailed theoretical study allows to calculate precisely the asymptotic parts of the potential curves. On the experimental point of view, we present the spectroscopy of the extern potential well of the caesium state O{sub g}{sup -}(6s+6p{sub 3/2}) and the construction of an effective potential curve of the RKR type. A unified theory of photo-association in weak field, considered as a collision assisted by laser, is developed. The cold atoms experiments allow to study and control the collision between two atoms whose mutual interaction is of the dipole-dipole type. Two different physical systems are studied: a sample of Rydberg atoms, and the photo-association process which is a laser-assisted collision. A modification of the motion of one pair of atoms makes it possible to control the bipolar forces and to choose the atoms relative speeds. (author)

  20. Network distribution: the future of propane gas; La distribution en reseau: l'avenir du gaz propane

    Energy Technology Data Exchange (ETDEWEB)

    Quesson, J. [Saint-Genis de Saintonge (France); Biaujou, A. [Butagaz (France); Berthelot, F. [Antargaz (France); Brunero, F. [Primagaz (France)

    2005-10-01

    The April 12, 1999 decree gave propane gas distributors the possibility to become public operators for distribution network. It enabled 28 000 French municipalities not supplied by natural gas network to have access to propane gas. What is the challenge for concession firms and for licensors? What has this evolution brought two years after the first public operators agreements signed by the French Ministry of Industry (number of certified operators, tenders for concessions, municipalities connected and number of subscribers)? What are the operator proposals in technical and tariff terms? Outlook for market evolution: number of subscribers connected in the two years to come?.

  1. Partitionnement d'un r\\'{e}seau de sociabilit\\'{e} \\`{a} fort coefficient de clustering

    CERN Document Server

    Boulet, R; Boulet, Romain; Jouve, Bertrand

    2006-01-01

    In order to compare social organization of a medieval peasantry before and after the Hundred Years' War we study the sructure of social networks built from a corpus of agrarian contracts. Low diameters and high clusterings show small-world graphs. Like many other networks studied these last years these graphs are scale-free. The distributions of the vertex degrees are fitted by a truncated power law. Moreover they have a rich-club : a dense core with a low diameter consisting of vertices with high degree. The particular shape of the laplacian spectrum allows us to extract communities that are spread along a star whose center is the rich-club.

  2. Optimization of the Algerian crude and condensate network; Optimisation du reseau algerien de transport de brut et de condensat

    Energy Technology Data Exchange (ETDEWEB)

    Smati, A.; Zeraibi, N. [Universite de Boumerdes, Institut des hydrocarbures et de la Chimie, Dept. Energetique, Boumerdes (Algeria); Touabti, M.C. [Universite de Setif, Lab. de Genie du Gaz, Setif (Algeria)

    2000-07-01

    The diagnosis and the optimal extension of the Algerian crude and condensate network, as a result of the escalation of production rhythms, necessitate the development of systemic procedures of modeling. In this context, the batching is represented by a two-commodity network flow. The theory of graphs and the maximal flow problem, associated with a model of reliability, are then put to contribution. The global model can then be used as an experimentation stand where the impact of any decision or event is released by simulation. However, because of the nonlinearity of costs, the optimal extension of the network is submitted to the resolution of a nonlinear programming model. (authors)

  3. D\\'efinition d'une structure adaptative de r\\'eseau local sans fil \\`a consommation optimis\\'ee

    CERN Document Server

    Chebira, Sabri; Francomme, Jackson

    2008-01-01

    The strong growth of low rate wireless personal area networks (LR-WPAN), leads us to consider the autonomy problems, thus node lifetime in a network, knowing that the power supplies replacement is often difficult to realize. The inherent mobility in this type of equipment is an essential element. It will provide routing constraints, so a complex problem to solve. This article provides work lines to assess the performance of such a network in terms of energy consumption and mobility. The objectives are contradictory; it will necessarily find a compromise. In addition, if we want to guarantee a maximum delay for the transmitted messages, possibility offered by the IEEE 802.15-4 standard, another compromise necessitate a strictly fixed structure and a fully mobile structure. Therefore, we present a quantization of the energy cost related to the desired data rate and compared to the sleep duration of nodes in the network. Then, we open reflexion lines to find the best compromise: consumption / mobility / guarante...

  4. Measures of protection against power network collapses; Les dispositions de defense contre les ecroulements du reseau electrique francais

    Energy Technology Data Exchange (ETDEWEB)

    Tesseron, J.M.; Testud, G.; Harmand, Y. [Reseau de transport de l' Electricite Francais, 75 Paris (France); Hoffmann, D. [Reseau de transport de l' Electricite Francais, Dir. du Groupe Exploitation Transport Sologne, 75 Paris (France)

    2002-10-01

    History shows that even the main industrialized countries may happen to face complex and rare disturbances liable to lead to a power system collapse. In order to avoid such situations, France has defined a policy to preserve power system reliability, which includes ensuring normal system operation, reducing the occurrence of incidents and avoiding system collapses, and mastering the consequences of a main incident. This article presents the basic principles of power system reliability, then the main current measures which are taken in the material, human and organisational fields. These measures are organized in defence lines, according to a 'in-depth defence' concept. (authors)

  5. The coupling of heavy mesons to the pion on the lattice; Couplage des mesons lourds au pion sur reseau

    Energy Technology Data Exchange (ETDEWEB)

    Herdoiza, G

    2004-04-15

    The QCD non-perturbative effects are among the main sources of uncertainty in our present knowledge of the Standard Model phenomenology. I will present some of the methods which can be used to study these effects, and I will particularly treat the case of lattice QCD. Effective theories can be combined to the lattice approach in order to study the chiral and the heavy quark sectors. I will give some examples of how these properties can be successfully applied to the quark flavour phenomenology. The coupling of heavy mesons to the pion is related to a non-perturbative quantity, noted g-bar, which is required to extract physical results from the effective theory combining both chiral and heavy quark symmetry. This coupling is also involved in the study of the form factors appearing in the heavy to light semi-leptonic decays. These heavy meson decays are used to extract some of the CKM matrix elements which are know, up to now, only with large uncertainties. Moreover, the chiral effects of heavy mesons depend on pion loops whose vertices are precisely the coupling g-bar. These are some of the reasons why the theoretical and experimental determination of this coupling is required. I will present the results of its studies on the lattice and I will compare them to those obtained through several other methods. I will therefore describe these different approaches, their limitations and possible improvements, both from the point of view of the method and of its application to the determination of the coupling g-bar. (author)

  6. Public utilities in networks: competition perspectives and new regulations; Services publics en reseau: perspectives de concurrence et nouvelles regulations

    Energy Technology Data Exchange (ETDEWEB)

    Bergougnoux, J

    2000-07-01

    This report makes first a status about the historical specificities, the present day situation and the perspectives of evolution of public utilities in networks with respect to the European directive of 1996 and to the 4 sectors of electricity, gas, railway transport and postal service. Then, it wonders about the new institutions and regulation procedures to implement to conciliate the public utility mission with the honest competition. (J.S.)

  7. Load management in electrical networks. Objectives, methods, prospects; Gestion de la charge du reseau electrique. Objectifs, methodes, perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Gabioud, D.

    2008-07-01

    This illustrated article takes up the problems related to the variation of the load in electricity networks. How to handle the peak load? Different solutions in the energy demand management are discussed. Method based on the price, method based on the reduction of the load by electric utilities. Information systems are presented which gives the consumer the needed data to participate in the local load management.

  8. Modelisation agregee de chauffe-eau electriques commandes par champ moyen pour la gestion des charges dans un reseau

    Science.gov (United States)

    Losseau, Romain

    The ongoing energy transition is about to entail important changes in the way we use and manage energy. In this view, smart grids are expected to play a significant part through the use of intelligent storage techniques. Initiated in 2014, the SmartDesc project follows this trend to create an innovative load management program by exploiting the thermal storage associated with electric water heaters existing in residential households. The device control algorithms rely on the recent theory of mean field games to achieve a decentralized control of the water heaters temperatures producing an aggregate optimal trajectory, designed to smooth the electric demand of a neighborhood. Currently, this theory does not include power and temperature constraints due to the tank heating system or necessary for the user's safety and comfort. Therefore, a trajectory violating these constraints would not be feasible and would not induce the forecast load smoothing. This master's thesis presents a method to detect the non-feasability, of a target trajectory based on the Kolmogorov equations associated with the controlled electric water heaters and suggests a way to correct it so as to make it achievable under constraints. First, a partial differential equations based model of the water heaters under temperature constraints is presented. Subsequently, a numerical scheme is developed to simulate it, and applied to the mean field control. The results of the mean field control with and without constraints are compared, and non-feasabilities of the target trajectory are highlighted upon violations. The last part of the thesis is dedicated to developing an accelerated version of the mean field and a method of correcting the target trajectory so as to enlarge as much as possible the set of achievable profiles.

  9. Validation and Verification of NATO Network Enabled Capabilities (Validation et verification des capacites reseau de l’OTAN (NNEC))

    Science.gov (United States)

    2008-07-01

    connectivité détecteur-tireur en temps voulu ; intégration RSTA maritime ; améliorations des échanges de données tactiques entre forces terrestres ...aériennes et navales nationales ; interface entre l’OTAN et les systèmes nationaux ; surveillance aérospatiale terrestre à distance, tous temps...5th Department – Technological Centre 305, rue Rideau, 9e étage Research Armaments Department Ottawa, Ontario K1A 0K2 Via XX Settembre 123 9-11

  10. Power electronics enter the electricity network arena; L'electronique de puissance entre sur le reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    Daniel, D.; Therond, P.G.; Lafon, L.

    1999-07-01

    Power electronics components have now reached a high enough level of performance to allow EDF to use them on their very high voltage lines. These components are to be found in the highly flexible, so-called 'FACTS' systems which will soon be providing network voltage and current control. (authors)

  11. Structure of distribution gas network operation system; Structure du systeme d'exploitation du reseau de distribution du gaz

    Energy Technology Data Exchange (ETDEWEB)

    Dzirba, E. [Institute of Oil and Gas (Poland); Osiadacz, A. [Warsaw Technical Uniwersity (Poland)

    2000-07-01

    Distribution networks have become increasingly complex to manage and potentially labor-intensive to operate safely and efficiently. Remote monitoring enables the operator to continually monitor the performance of the system from a central point(s). Where the system operates outside known parameters, the operator can respond very quickly. Remote control provides the additional functionality of enabling plant to be operated from a central point(s) directly or indirectly via operator. Communication can be through one or a combination of different communication media. The whole system for operation of gas distribution network may be looked at as 5-level general model based on ISO proposals adapted for modern control and management systems. The structure and requirements imposed on such a system are described in this paper. (authors)

  12. \\'Evaluation d'une application de transmission d'images m\\'edicales avec un r\\'eseau sans fil

    CERN Document Server

    Francomme, Jackson; Chebira, Sabri

    2008-01-01

    We offer a platform for database consultations and/or biomedical images exchanges, adapted to the low rate wireless transmission, and intended for general practitioners or specialists. The goal can be preventive, diagnostic and therapeutic. it Concerns specialties such as radiology, ultrasound, the anatomical pathology or endoscopy. The main features required in such a context are to adjust the data compression of both the specific needs of telemedicine and limited capabilities of wireless communication networks. We present our approach in which we have set out criteria on Biomedical images quality, compressed by the wavelet method to retain all the necessary information for an accurate diagnosis, and determined the characteristics of a wireless network with minimal performances for the transmission of these images within constraints related to the modality and the data flow, in this case Wifi based on the IEEE 802.11 standard. Our results will assess the capacity of this standard in terms of speed, to transm...

  13. Final synthesis of Sarnet (Phase 1) corium activities; Synthese finale des activites corium du reseau Sarnet (Phase 1)

    Energy Technology Data Exchange (ETDEWEB)

    Journeau, Ch. [CEA Cadarache, 13 - Saint Paul lez Durance (France); Steinbruck, M. [Forschungszentrum Juelich GmbH (Germany); Repetto, G.; Duriez, Ch.; Koundy, V. [Institut de Radioprotection et de Surete Nucleaire (IRSN), 92 - Clamart (France); Ma, W.M. [Kungliga Tekniska Hoegskolan (KTH), Stockholm (Sweden); Burger, M. [Institut fur Kernenergetik und Energiesysteme, Stuttgart (IKE) (Germany); Spindler, B. [CEA Grenoble, 38 (France)

    2009-07-01

    Within the SARNET Severe Accident Research Network of excellence, the Corium topic covers all the behaviour of corium (mixture formed by the molten materials arising from a postulated nuclear reactor severe accident) from early phase of core degradation to in or ex-vessel corium recovery with the exception of corium interaction with water, direct containment heating and fission product release. The Corium topic regroups in three work packages the critical mass of competence to improve significantly the corium behaviour knowledge. The spirit of the SARNET networking is to share the knowledge, the facilities and the simulation tools for severe accidents, so to reach a better efficiency and to rationalize the R and D effort at European level. Extensive benchmarking has been launched in most of the areas of research. These benchmarks were mainly dedicated to the recalculation of analytical experiments, integral experiments or reactor applications. Eventually, all the knowledge will be accumulated in the ASTEC severe accident simulation code through physical model improvements and extension of validation database. This report summarizes the progress that has been achieved in the frame of the networking activities for the four and half years of the FP6 project. (authors)

  14. Deploiement et gestion d'un reseau de noeuds multimedias embarques et distribues dans un habitat intelligent

    Science.gov (United States)

    Stein, Kilian

    Les laboratoires de recherche sur l'assistance aux personnes âgées ou avec une déficience cognitive possèdent actuellement peu de solutions réellement déployables dans un habitat. Ils se heurtent donc à un problème de transfert de leurs applications vers des conditions réelles. En effet, la plupart de ces applications ont besoin de serveurs et d'équipements coûteux ou complexes à installer. La nouvelle tendance des laboratoires de recherche est donc à l'élaboration de solutions plug and play. En effet, ces dernières sont facilement et rapidement installables dans un habitat. C'est dans cette optique que mon présent projet de recherche a été réalisé. L'objectif de cette maîtrise est de proposer une solution à ce problème en déployant des plateformes embarquées pouvant supporter des applications multimédias distribuées. Ces plateformes appelées noeuds multimédias ont la particularité d'être simples d'installation, peu intrusives et de faibles coûts. Afin de tester la gestion du réseau de noeuds multimédias, une application distribuée de suivi multimédia à travers un habitat intelligent a été réalisée dans l'appartement du laboratoire DOMUS. Mots-clés : réseau, distribué, multimédia, embarqué, habitat intelligent, déploiement, gestion, suivi multimédia.

  15. Development of Instrumentation-aided Design & Management System for HTR-PM%HTR-PM仪表辅助设计管理系统的应用开发

    Institute of Scientific and Technical Information of China (English)

    熊德桂

    2014-01-01

    Due to high temperature gas-cooled reactor demonstrative project itself is a complicated system engineering, in order to ensure that the consistency and accuracy of multifarious instrumentation design data, and improve the design quality and efficiency, and avoid the design data misuse, based on the investigation of the technological status and research trends of the domestic and foreign large-scale engineering company, an instrument aided design & management system based on INtools for the high temperature gas-cooled reactor nuclear power plant was developed.%为了保证高温气冷堆核电站示范工程仪表设计项的大量繁杂的设计数据的一致性和准确性,提高设计质量与效率,避免出现设计数据的误用,本文经过广泛调研国内外大型工程的解决方案,借助国际先进的设计软件工具——INtools,经过二次开发,成功构建了一个适用于高温气冷堆核电站示范工程的仪表辅助设计管理系统。

  16. Some applications of the Faddeev-Yakubovsky equations to the cold-atom physics; Quelques applications des equations de Faddeev-Yakubovsky a la physique des atomes froids

    Energy Technology Data Exchange (ETDEWEB)

    Carbonell, J. [Laboratoire physique subatomique et cosmologie, universite Jospeh-Fourier, CNRS/IN2P3, 53, avenue des Martyrs, 38026 Grenoble cedex (France); Deltuva, A. [Centro de Fisica Nuclear da Universidade de Lisboa, P-1649-003 Lisboa (Portugal); Lazauskas, R. [IPHC, IN2P3-CNRS/universite Louis-Pasteur, BP 28, 67037 Strasbourg cedex 2 (France)

    2011-01-15

    We present some recent applications of the Faddeev-Yakubovsky equations in describing atomic bound and scattering problems. We consider the scattering of a charged particle X by atomic hydrogen with special interest in X = p,e{sup {+-},} systems of cold bosonic molecules and the bound and scattering properties of N=3 and N=4 atomic {sup 4}He multimers. (authors)

  17. Blowing Hot and Cold: Protecting Against Climatic Extremes (Souffler le chaud et le froid: comment se proteger contre les conditions climstiques extremes)

    Science.gov (United States)

    2002-04-01

    several papers on chemical protection, one could recognise the competition between ‘protection’ researchers and ‘thermal’ researchers. Protection...Katch VL. Exercise Physiology: Energy, Nutrition , and Human Performance, 3rd Ed. Philadelphia: Lea & Febiger, 1991, p. 61-62, 216-222, 810-1. 12...had become habituated, but unlike the Inuits and Lapps, vasoconstrictor responses had not. Long-distance swimmers, surfers and scuba divers show a

  18. Experimental study of a finned-tubes coil with wet wall conditions; Etude experimentale d'une batterie froide en condition de paroi mouillee

    Energy Technology Data Exchange (ETDEWEB)

    Khalfi, M.S. [Institut Algerien du Petrole, Boumerdes (Algeria); Benelmir, R.; Feidt, M. [Universite Henri Poincare, Lab. d' Energetique et de Mecanique Theorique et Appliquee, 54 - Vandoeuvre-les-Nancy (France)

    2001-01-01

    The use of a global pseudo-conductance in the mathematical model of heat and mass transfer within a heat exchanger allowed us to take into account the water condensate on the wall as well as air humidity. The application of this dual consideration to our experimental investigation shows a 'critical' Reynolds number at which we observe an inversion of thermal behavior. The heat exchanger considered in this work has been used in a previous published work where heat transfer occurred only with dry wall condition. (authors)

  19. Management of Heat and Cold Stress -- Guidance to NATO Medical Personnel (Gestion des contraintes thermiques (chaleur et froid) Conseils aux personnels medicaux de I’OTAN)

    Science.gov (United States)

    2013-12-01

    Brussels BELGIUM Christian.carton@mil.be Prof. Dr. Vahur Ööpik Institute of Exercise Biology and Physiotherapy Estonian Centre of Behavioural and...including prior history of heat stroke , use of medications, alcohol or drugs abuse). Fit, healthy, heat acclimatized, fully hydrated, and well-rested...casualties (heat exhaustion and heat stroke ). For these casualties, preparation should include arrangements for rapid cooling (cool or cold water

  20. The Support of Air Operations Under Extreme Hot and Cold Weather Conditions (Les Operatons Aeriennes en Environnement Extreme Chaud/Froid)

    Science.gov (United States)

    1993-10-01

    foedsIte. Stndad ECO lcharodexskW w The caociate over the T10 wean affixed In the iaet, and the -I "jec then second 5- imte perod by plicction of the Weir...8217in terms of body 6nquoly and 10. Pugh, LA.CK and Bdholm, O.G., -The IK with ofime ofotaig Physiology of Channel swimmers ", Lancet ii, 1955, pp ,Atim...May1993. My next experience with seasick- By totally incapacitated, I mean 4 ness was in 1982 when I started that without our rescue swimmers to teach at

  1. Effets du froid sur les stades précoces de développement du maïs (Zea mays L. (synthèse bibliographique

    Directory of Open Access Journals (Sweden)

    Riva-Roveda, L.

    2015-01-01

    Full Text Available Effects of cold temperatures on the early stages of maize (Zea mays L.. A review. Introduction. Maize is a crop of great economical importance. Despite its tropical origin and its high sensitivity to low temperatures, maize is now cultivated in a wide range of latitudes. To maintain competitive yields, many genetic and agricultural adaptations have been implemented. Literature. Plant responses to environmental stresses such as drought or cold have been extensively studied. This article reviews the progress and current knowledge regarding the effects of non-freezing cold temperatures on maize plants, from germination to floral transition. During the early stages of development, cold alters plant phenology and productivity, mainly because of its negative impact on photosynthesis. Plant growth is affected and secondary osmotic and oxidative stresses induce cell damage. Conclusion. A better understanding of the effects of cold will allow the development of new strategies for improving plant tolerance through the use of various physiological, genetic and molecular approaches.

  2. Coldness distribution by stabilized ice slurries. Study of the behaviour under thermal cycling; Distribution du froid par coulis de glace stabilisee. Etude du comportement sous cyclage thermique

    Energy Technology Data Exchange (ETDEWEB)

    Jacquier, D.

    2004-10-01

    The purpose of this work is to study a two-phase secondary refrigerant composed of phase-change particles suspended in a carrying liquid. This mixture has been hydraulically and thermally characterised. Moreover, some visualizations of flow patterns have been performed. Measurements of pressure losses have been realised in the case of solid state of the particles and in the case of liquid state. Heat transfer balances allowed us to show an improvement of a 1,9 factor before phase-change, in comparison with the case of a pure carrying liquid (without any particles). Flow patterns, which were theoretically specified, in function of fluid speed, have been observed experimentally. (author)

  3. French nuclear dissuasion after the cold war: continuity, ruptures, questions; La dissuasion nucleaire francaise apres la guerre froide: continuite, ruptures, interrogations

    Energy Technology Data Exchange (ETDEWEB)

    Tertrais, B

    2000-07-01

    Nuclear dissuasion is a slow and permanent process of adjustment to the strategic environment. French dissuasion adaptation to the new international environment covers a full decade, starting in the 1989-1992 era with a re-evaluation of defense programs. It has been followed by the 1994 defense white book which opens up the European perspective and reaches its peak in 1996 with a series of major decisions: renouncement of surface-to-surface missiles, launching of the M51 program, end of nuclear tests and shift towards simulation, dismantling of fissile materials production facilities etc. This process shows up two logics: the one of continuity with the confirmation of the bases of the French nuclear doctrine, and the one of discontinuity with significant changes in the general domain of the French nuclear policy. It also opens up questions about the field of application of dissuasion and the future of the nuclear consensus. (J.S.)

  4. Greenhouse effect: effects on refrigerating and air conditioning industries; Effet de serre: impacts sur les professions du froid et de la climatisation

    Energy Technology Data Exchange (ETDEWEB)

    Le Boru, B. [Association Francaise de Froid, Alliance Froid Climatisation Environnement, 75 - Paris (France)

    1997-12-31

    The various factors (refrigerant characteristics, design and operating performance, insulation type, etc.) involved in greenhouse gas emission from refrigerating and air conditioning equipment are listed with the potential actions that may be taken at the different stages of equipment design, engineering, installation, operation, maintenance and dismantling, in order to reduce pollutant emissions

  5. Greenhouse effect: an issue for the refrigeration and air conditioning sector; Effet de serre: quelle problematique pour le froid et le conditionnement de l`air?

    Energy Technology Data Exchange (ETDEWEB)

    Billiard, F. [Institut International du Froid, 75 - Paris (France)

    1997-12-31

    The principles of greenhouse effect and the greenhouse gas main direct and indirect emission sources due to refrigeration and air conditioning systems are first reviewed. Evolution scenarios from 1992 to 2020 and 2100 for the emissions of CFC, HCFC and HFC are presented and related to the Kyoto protocol project limitations; technical improvements in refrigerating and air conditioning systems (lower refrigerant utilization, fluid confinement, alternative technologies, natural refrigerant utilization, etc.) could lead to substantial diminutions of these greenhouse gases

  6. The Hanbury Brown ant Twiss effect for cold atoms; L'effet Hanbury Brown et Twiss pour les atomes froids

    Energy Technology Data Exchange (ETDEWEB)

    Schellekens, M

    2007-05-15

    This thesis deals with the measurement of the quantum intensity correlations in gases of metastable Helium. The measurement has been performed on thermal gases of bosonic He{sup 4} and fermionic He{sup 3}, as well as on Bose-Einstein condensates. In 1956, Robert Hanbury Brown et Richard Twiss measured the correlation between photons emitted from a single thermal source. The consequently demonstrated that the photons emitted by such a source tend to arrive grouped on a detector (Hanbury Brown and Twiss effect). This bunching characterizes bosons from a non-coherent source. Fermions show an anti-bunching behaviour in the same conditions. By using metastable Helium atoms, that can be detected individually through the use of micro-channel plates, we have been able to show a similar bunching of bosons He{sup 4} from thermal sources around the micro-kelvin. As expected, the coherence of the Bose-Einstein condensates did not produce a particular correlation. The measurement on thermal gases of fermionic He{sup 3} has demonstrated the anti-bunching. Particular effort has been employed in describing the micro-channel plate based delay-line detector, the key to the experiment. (author)

  7. Modélisation des installations de fusion en creuset froid pour l'élaboration en continu ou la lévitation

    OpenAIRE

    Gagnoud, Annie

    1986-01-01

    Cold crucibles are used to elaborate highly reactive materials in conditions of high purity. Little industrially exploited, their design is driven by empiricism. Electromagnetic modeling of both cylindrical and levitation cold crucibles is performed. The method requires only the discretization of the electrically active parts and takes into account the three-dimensional character of the geometry of the crucibles. Valuable lessons are derived which allow computer aided design of melting facili...

  8. Dynamic behavior of the HTR-10 reactor: Dual temperature feedback model

    Directory of Open Access Journals (Sweden)

    Hosseini Seyed Ali

    2015-01-01

    Full Text Available The current work aims at presenting a simple model for PBM-type reactors' dynamic behavior analysis. The proposed model is based on point kinetics equations coupled with feedbacks from fuel and moderator temperatures. The temperature reactivity coefficients were obtained through MCNP code and via available experimental data. Parameters such as heat capacity and heat conductivity were carefully analyzed and the final system of equations was numerically solved. The obtained results, while in partial agreement with previously proposed models, suggest lower sensitivity to step reactivity insertion as compared to other reactor designs and inherent safety of the design.

  9. K{sub {infinity}} measurements of HTR lattices by oscillated zero reactivity technique

    Energy Technology Data Exchange (ETDEWEB)

    Benedetti, F.; Brighenti, G.; Chiodi, P.L.; Giuliani, C.

    1973-10-15

    Referring to 9th DCPM and 15th DCPM (AGN), the measurements program has been scheduled as foreseen, with some variations suggested by the experience development. The measurements are scheduled to be completed by the end of 1973, but we have judged interesting to present some of the results obtained to date.

  10. Testing of high temperature materials within HTR program in Czech Republic

    Directory of Open Access Journals (Sweden)

    Berka Jan

    2016-01-01

    Full Text Available Research institutes and also industrial companies in Czech Republic are involved in High Temperature Gas Cooled Reactor (HTGR program and activities related to the study of advanced materials and HTGR technologies. These activities are supported by EC (within international projects, e.g. FP7-ARCHER, ALLIANCE, GoFastR can be mentioned and also by Technology Agency of Czech Republic. Within these activities, degradation of metallic and ceramic materials in the high temperature helium atmosphere is investigated, and also new experimental facilities for material testing are built. As examples of tested materials, Alloy 800 H, ferritic steel P91, austenitic steel 316, Inconel 713 and 738 and corundum ceramics could be named. The selected results of exposure experiments in the high temperature helium environment are presented in this paper.

  11. Fracture of a HTR-PMI cranioplastic implant after severe TBI.

    Science.gov (United States)

    López González, Antonio; Pérez Borredá, Pedro; Conde Sardón, Rebeca

    2015-02-01

    A 13-year-old girl with a large left fronto-parietal hard-tissue replacement patient-matched implant (HTR®-PMI) cranioplasty-since she suffered from a traumatic brain injury (TBI) 6 years ago-had a new severe TBI that detached and fractured the implant as well as caused a left subdural hematoma and a large frontal contusion. The hematoma and contusion were removed and the implant was substituted by a provisional titanium mesh. To the best of our knowledge, this is the first case reported about an HTR®-PMI fracture. It is theorized that the bone ingrowth into the macroporous implants, like those of hydroxyapatite, gives strength and resistance to the implant. But in the case we describe, no macroscopic bone ingrowth was detected 6 years after implantation and the traumatic force that impacted over the cranioplasty exceeded its properties.

  12. Valproate, thalidomide and ethyl alcohol alter the migration of HTR-8/SVneo cells

    OpenAIRE

    Rout Ujjwal K

    2006-01-01

    Abstract Background Valproate, thalidomide and alcohol (ethanol) exposure during the first trimester of pregnancy is known to cause several developmental disorders. All these teratogens are known to pass the placental barrier and interfere directly with the normal development of the fetus. However, these teratogens also alter the formation and function of the placenta itself which may in turn affect the proper nourishment and development of the fetus. Optimum development of the placenta requi...

  13. The HtrA-Like Protease CD3284 Modulates Virulence of Clostridium difficile

    NARCIS (Netherlands)

    Bakker, Dennis; Buckley, Anthony M.; de Jong, Anne; van Winden, Vincent J. C.; Verhoeks, Joost P. A.; Kuipers, Oscar P.; Douce, Gillian R.; Kuijper, Ed J.; Smits, Wiep Klaas; Corver, Jeroen

    2014-01-01

    In the past decade, Clostridium difficile has emerged as an important gut pathogen. Symptoms of C. difficile infection range from mild diarrhea to pseudomembranous colitis. Besides the two main virulence factors toxin A and toxin B, other virulence factors are likely to play a role in the pathogenes

  14. HTR-Based Power Plants’ Performance Analysis Applied on Conventional Combined Cycles

    Directory of Open Access Journals (Sweden)

    José Carbia Carril

    2015-01-01

    Full Text Available In high temperature reactors including gas cooled fast reactors and gas turbine modular helium reactors (GT-MHR specifically designed to operate as power plant heat sources, efficiency enhancement at effective cost under safe conditions can be achieved. Mentioned improvements concern the implementation of two cycle structures: (a, a stand alone Brayton operating with helium and a stand alone Rankine cycle (RC with regeneration, operating with carbon dioxide at ultrasupercritical pressure as working fluid (WF, where condensation is carried out at quasicritical conditions, and (b, a combined cycle (CC, in which the topping closed Brayton cycle (CBC operates with helium as WF, while the bottoming RC is operated with one of the following WFs: carbon dioxide, xenon, ethane, ammonia, or water. In both cases, an intermediate heat exchanger (IHE is proposed to provide thermal energy to the closed Brayton or to the Rankine cycles. The results of the case study show that the thermal efficiency, through the use of a CC, is slightly improved (from 45.79% for BC and from 50.17% for RC to 53.63 for the proposed CC with He-H2O operating under safety standards.

  15. The association between HTR2C gene polymorphisms and the metabolic syndrome in patients with schizophrenia.

    NARCIS (Netherlands)

    Mulder, H.; Franke, B.; Beek, A.A. van der; Arends, J.; Wilmink, F.W.; Scheffer, H.; Egberts, A.C.G.

    2007-01-01

    The use of antipsychotics is associated with metabolic side effects, which put patients with schizophrenia or related disorders at risk for cardiovascular morbidity. The high interindividual variability in antipsychotic-induced metabolic abnormalities suggests that genetic makeup is a possible deter

  16. Thermochemical cycles for the heat and cold long-range transport. Final report of the PRI 9.2 Cold transport. Annual report of the PR 2-8; Cycles thermochimiques pour le transport de chaleur et de froid a longue distance. Rapport final du PRI 9.2. Transport de froid. Rapport annuel du PR 2-8

    Energy Technology Data Exchange (ETDEWEB)

    Luo, L.; Tondeur, D. [Laboratoire des Sciences du Genie Chimique (LSGC), 54 - Nancy (France); Mazet, N.; Neveu, P.; Stitou, D.; Spinner, B. [Institut de Science et de Genie des Materiaux et Procedes (IMP), 66 - Perpignan (France)

    2004-07-01

    This PRI deals with the use of thermochemical processes, based on solid-gas reversible transformation, to transfer heat of cold at long-range distance (> 10 km), in order to enhance the energy efficiency. Four main aspects have been studied to confirm the process feasibility: the process identification and the operating conditions, the selection of compatible reagents, the design of an auto-thermal reactor and the gas transport impact on the global performances. (A.L.B.)

  17. Cold distribution networks. Cold distribution by two-phase refrigerant fluid. Case of ice slurries; Reseaux de distribution de froid. Distribution de froid par fluide frigoporteur diphasique. Cas du coulis de glace stabilisee (2002 - 2004). Rapport final (1er juillet 2004). Programme energie. PRI 9.1

    Energy Technology Data Exchange (ETDEWEB)

    Lallemand, A. [Institut National des Sciences Appliquees (INSA), Centre de Thermique de Lyon (CETHIL), UMR 5008, 69 - Villeurbanne (France); Mercier, P. [CEA Grenoble, Groupement pour la Recherche sur les Echangeurs Thermiques (GRETH), 38 (France); Royon, L. [Paris-1 Univ., Lab. de Biorheologie et d' Hydrodynamique Physico-Chimique, 75 (France); Dumas, J.P. [L' Ecole Nationale Superieure en Genie des Technologies Industrielles, Lab. de Thermique Energetique et Procedes, 64 - Pau (France); Guilpart, J. [URGPAN/CEMAGRE, 33 - Bordeaux (France)

    2004-07-01

    This PRI aims to participate to the development of alternate solutions for refrigerant fluids, for the cold transport and more specially a two phase refrigerant fluid: the stabilized ice slurry. The research program presented three main axis: design, realization and characterization of stabilized ice slurry, experimental studies of transport and transfer properties, study of online measurement process of the solid content. A simulation has been realized to evaluate the energy efficiency of this refrigerant use. (A.L.B.)

  18. Cold storage in a two-phases fluid for air-conditioning and/or industrial processes - Feasibility study; Stockage de froid par fluide diphasique pour transport et distribution du froid, pour la climatisation et/ou processus industriels. Etude de faisabilite II - Rapport

    Energy Technology Data Exchange (ETDEWEB)

    Hu, J.; Sari, O.; Brun, F.; Eicher, S.

    2008-07-01

    In the present report, 2 large-scale continuous CO{sub 2} hydrate formation-dissociation conditions were experimentally investigated on a fully functional laboratory demonstrator for industrial applications. The solid fraction of CO{sub 2} hydrate slurry was evaluated by a numerical approach based on a CO{sub 2} mass balance. A first CO{sub 2} hydrate slurry production system with compressor was tested. In this system the CO{sub 2} hydrate slurry formation and dissociation pressure, temperature, density and dynamic viscosity were measured. The consumption of CO{sub 2} hydrate slurry in the consumer installed in the climatic room was successful. The continuous formation and dissociation processes in this loop were not very successful. A second experimental loop for hydrate production without compressor was completed. The new test rig is an industrial prototype approach. The new test rig is simple, easy to be modified and adapted to new devices. The Axima heat exchanger was modified and is being used for hydrate slurry production. Kenics mixing elements and Sulzer SMV gas mixers were tested in the loop. Experimental results showed that the mixing elements and gas mixers can promote hydrate formation. CO{sub 2} hydrate slurry density was measured with a value of 1032 kg/m{sup 3} which corresponds to over 30% solid fraction (150 kJ/kg dissociation enthalpy of water). The power of the pump was tested and it was showed that it is not sufficient to pump CO{sub 2} hydrate slurry when the density is over 1030 kg/m{sup 3}. The specific heat of water and of ice, the enthalpy of ice and of CO{sub 2} hydrates were obtained using a differential scanning calorimetry, Micro DSCVII. It confirmed that CO{sub 2} dissolved in water depresses the freezing point of water. On-line measurements of corrosion rates were performed and reliable results obtained. (author)

  19. Development scheme of the public power transportation network; Le schema de developpement du reseau public de transport d'electricite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Article 14 of the modified law from February 10, 2000 relative to the modernization and development of the electric utility foresees that the development scheme of the public power transportation network is regularly submitted to the approval of the ministry of energy after advice from the energy regulation commission. The development scheme identifies the areas of 'power fragility' with respect to the existing or future constraints susceptible to occur at the short- or medium-term on the French power grid. This document comprises the text of the law 2000-108 from February 10, 2000, and the complete development scheme with its appendixes (regulatory and administrative context relative to network projects, constraints relative to each administrative region). (J.S.)

  20. Simulation of lung motions using an artificial neural network; Utilisation d'un reseau de neurones artificiels pour la simulation des mouvements pulmonaires

    Energy Technology Data Exchange (ETDEWEB)

    Laurent, R.; Henriet, J.; Sauget, M.; Gschwind, R.; Makovicka, L. [IRMA/ENISYS/FEMTO-ST, UMR 6174 CNRS, pole universitaire des Portes du Jura, BP 71427, 25211 Montbeliard cedex (France); Salomon, M. [AND/LIFC, universite de Franche-Comte, BP 527, rue Engel-Gros, 90016 Belfort cedex (France); Nguyen, F. [Service de radiotherapie, CHU Jean-Minjoz, 3, boulevard Fleming, 25030 Besancon cedex (France)

    2011-04-15

    Purpose. A way to improve the accuracy of lung radiotherapy for a patient is to get a better understanding of its lung motion. Indeed, thanks to this knowledge it becomes possible to follow the displacements of the clinical target volume (CTV) induced by the lung breathing. This paper presents a feasibility study of an original method to simulate the positions of points in patient's lung at all breathing phases. Patients and methods. This method, based on an artificial neural network, allowed learning the lung motion on real cases and then to simulate it for new patients for which only the beginning and the end breathing data are known. The neural network learning set is made up of more than 600 points. These points, shared out on three patients and gathered on a specific lung area, were plotted by a MD. Results. - The first results are promising: an average accuracy of 1 mm is obtained for a spatial resolution of 1 x 1 x 2.5 mm{sup 3}. Conclusion. We have demonstrated that it is possible to simulate lung motion with accuracy using an artificial neural network. As future work we plan to improve the accuracy of our method with the addition of new patient data and a coverage of the whole lungs. (authors)

  1. Tar in road system wastes - bibliographic study - quick characterization methods; Le goudron dans les dechets du reseau routier - etude bibliographique - methodes de caracterisation rapides

    Energy Technology Data Exchange (ETDEWEB)

    Brazillet, C.; Domas, J.; Pepin, G.

    2001-12-15

    In the framework of the european regulations on the wastes management, the road structure wastes present a particularly problem because of the tar content and of the toxic associated polycyclic aromatic hydrocarbons. In order to chose the elimination or the valorization of these roads wastes, it is necessary to measure the tar content. After a presentation of the study objectives, a bibliographic study of the context, the challenges, the hydrocarbons, the tar characterization and the today situation of the french road network, the author presents the methods of PAH detection with a special attention on the well known methods: the PAK marker, the TSE and the toluene spot. (A.L.B.)

  2. Safety Network to Detect Performance Degradation and Pilot Incapacitation (Reseau de securite pour detecter la degradation des performances et la defaillance du pilote)

    Science.gov (United States)

    1990-09-01

    stengthening the common defence posture: - Improving the co-operation tnmong member nations in aerospace research and devtlopment; - Exchange. of scientific...Since the center of gravity of the head/helmet is not aligned with its support (the neck ) the head will slump in whatever direction is dictated by the

  3. Modeling of a tram power network: from the component to the system; Modelisation d'un reseau electrique de tramway: du composant au systeme

    Energy Technology Data Exchange (ETDEWEB)

    Morin, E.

    2005-01-15

    The study and the modeling of tram power networks require a system analysis. The use of several power-electronic converters in these networks significantly modifies both their topology and their function. Therefore, modeling methods developed in this thesis differ from traditional approaches, since a global analysis of a system should be linked with the study of its components.To satisfy the demands of the tram domain, this thesis develops modeling methods for transmission lines and transformers. Two levels of analysis are considered. Firstly, the tram power network is evaluated in relation to time. Secondly, an Iterative Harmonic Analysis (IHA) program is developed to compute the spectra of the system accurately. (author)

  4. \\'Evaluation et consolidation d'un r\\'eseau lexical via un outil pour retrouver le mot sur le bout de la langue

    CERN Document Server

    Joubert, Alain; Schwab, Didier; Zock, Michael

    2012-01-01

    Since September 2007, a large scale lexical network for French is under construction through methods based on some kind of popular consensus by means of games (JeuxDeMots project). Human intervention can be considered as marginal. It is limited to corrections, adjustments and validation of the senses of terms, which amounts to less than 0,5 % of the relations in the network. To appreciate the quality of this resource built by non-expert users (players of the game), we use a similar approach to its construction. The resource must be validated by laymen, persistent in time, on open class vocabulary. We suggest to check whether our tool is able to solve the Tip of the Tongue (TOT) problem. Just like JeuxDeMots, our tool can be considered as an on-line game. Like the former, it allows the acquisition of new relations, enriching thus the (existing) network.

  5. Long-term transport network 2008-2017 investment forecast plan; Plan previsionnel d'investissements a long terme 2008-2017 sur le reseau de transport

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    Total Infrastructure Gaz France (TIGF) provides and develops natural gas transport and storage services on a European scale to meet its customers' needs. It achieves a turnover of almost 300 million Euros and employs some 370 people. Backed by 50 years of expertise, TIGF is a major player in the natural gas market in 15 departements in South-West France. TIGF has drawn up an indicative development plan for the network for 2008-2017. This document gives an overview of proposed investments and of the development of transport services provided by TIGF, responding to changes in the gas market, the shippers' growing need for transport capacity and the forecast growth in gas consumption in the TIGF area (proposed CCGT gas fired power stations). Investment in gas infrastructure is a major step forward towards assuring the development of a competitive market that is fair, transparent and non-discriminatory for the years to come. That is why TIGF is focussing its development in the coming years on increasing the fluidity of transits entering/leaving its area. As the gas market is currently in a state of continuous change, and major uncertainties hang over the needs of the various players particularly over exchanges with the area to the north of TIGF, with Spain and the installation of a methane terminal at Verdon. In this uncertain climate, TIGF will focus on developing the WEST corridor for the next 5 years (2008- 2013), to provide reversibility of flows between France and Spain. This will cover the following works: the LACAL pipeline (Lacq-Calahorra), the Bearn Pipeline Artery (Lussagnet - Lacq), increased capacity on the Guyenne Pipeline Artery and on the EUSKADOUR pipeline (Coudure - Arcangues). This pipeline corridor, on which TIGF's development work started in 2007, is currently the one at the most advanced stage. For the next few years it will become the sole exchange hub between northern Spain and southern France. Beyond 2013, depending on market developments, TIGF has quantified the following projects: - The development of the existing EUSKADOUR link (Lussagnet - Bilbao connection) to increase exchange capacity on the Western Pipeline Corridor. - The development of a new connection with Spain to the east of the TIGF area (MIDCAT project (Midi - Catalonia)). If the market, which will be consulted in the near future, shows itself to be attracted to capacities in excess of those developed on the Western Corridor, then TIGF may decide to develop a new interconnection to the East of its area between Le Perthus and Barbaira (Carcassonne)

  6. Conception et realisation d'un recepteur compose de reseau d'antennes Yagi multicouches verticales et de composants en ondes millimetriques

    Science.gov (United States)

    Kramer, Olivier

    Millimeter wave applications such as high-speed wireless connections require modular, compact-size, low-cost and high-performance systems. In order to realize a complete receiver satisfying those requirements, compact stacked multilayered designs are presented in this thesis. First, high-gain compact stacked multilayered Yagi designs are proposed and demonstrated at 5.8 GHz. The structure makes use for the first time of vertically stacked Yagi-like parasitic director elements that allow easily obtaining a simulated gain of 12 dBi. Two different antenna configurations are presented, one based on dipole geometry for single polarization, and the other on a circular patch to achieve dual polarization. Measured results of the fabricated antenna prototypes are in good agreement with simulated results. Second, based on the above-demonstrated principle, the antenna is redesigned and adapted for 60 GHz applications, and a novel design showing for the first time an array of Yagi elements in millimeter wave stacked structure is presented. An analysis is performed to define the structure limits. The measured element attains 11 dBi of gain. The proposed 4x4 array has a size of 50x50x60 mm3, and reaches a measured gain of 18 dBi over 7% of bandwidth. An alternative configuration of the array using angled Yagi antenna elements allows for a significant improvement of the side lobe level without a visible impact on the gain. The proposed antennas present excellent candidates for integrated low-cost millimeter-wave systems that require small footprint. Third, a novel dual layered six-port front-end circuit using the Substrate Integrated Waveguide (SIW) technology is presented and demonstrated. The six-port architecture makes use of multilayer couplers, providing a wide coupling area through two slots; a new broadband SIW phase shifter composed of two H-plane stub lines and one reference line; and two SIW power dividers. Simulation and measurement results show that the proposed six-port circuit can easily operate at 60 GHz for V-band system applications. The complete QPSK demodulation is tested through the ADS simulation platform to prove the good performances of the designed circuits.

  7. D\\'eveloppement et analyse multi outils d'un protocole MAC d\\'eterministe pour un r\\'eseau de capteurs sans fil

    CERN Document Server

    Val, Thierry

    2008-01-01

    In this article, we present a multi-tool method for the development and the analysis of a new medium access method. IEEE 802.15.4 / ZigBee technology has been used as a basis for this new determinist MAC layer which enables a high level of QoS. This WPAN can be typically used for wireless sensor networks which require strong temporal constraints. To validate the proposed protocol, three complementary and adequate tools are used: Petri Nets for the formal validation of the algorithm, a dedicated simulator for the temporal aspects, and some measures on a real prototype based on a couple of ZigBee FREESCALE components for the hardware characterization of layers #1 and #2.

  8. Industrial computer science. A thermolysis pilot unit has opted for the `Fieldbus` field network; Informatique industrielle. Une unite pilote de thermolyse adopte le reseau de terrain Fieldbus

    Energy Technology Data Exchange (ETDEWEB)

    Gauthier, F.

    1998-03-01

    The field network from the US Fieldbus Foundation is poorly known in France. The Societe Francaise de Thermolyse company (French Society of Thermolysis, Chateaurenard, Bouches du Rhone, France) has decided to install it on a pilot-facility using equipments provided by the Smar company. This pilot-facility is devoted to the processing of industrial wastes for their conversion into storable fuels (gas, hydrocarbons, coal) using thermolysis principle. The field bus is used to supervise and control the entire process. (J.S.)

  9. Forecast on the development of a transport network 2008-2017; Etude prospective sur le developpement du reseau de transport 2008-2017

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    This study on the gas transport network for 2008-2017 is presented in five parts. The first part presents the main characteristics of the GRTgas network and capacity sizing. The second part details the elements presenting a possible impact on the network development. The policy and strategy concerning the infrastructure development are presented in the third part. The part four deals with the development projects needed to the satisfaction of the market capacity supply. The last part is a synthesis of the investments on the period. (A.L.B.)

  10. Electrical engineering. From the grid to the converter. Learning with examples; Genie electrique. Du reseau au convertisseur. Apprendre par l'exemple

    Energy Technology Data Exchange (ETDEWEB)

    Cocquerelle, J.L.

    1996-07-01

    This book deals with the analysis of power electronic converters and of their conducted disturbances, in particular the emission of harmonic currents. The basic electrical and electrotechnical knowledge is recalled first (power distribution systems, transformers..) and then the different types of converters are presented in a first chapter and illustrated with a description and a numerical analysis of each of them: DC-DC, AC-AC, AC-DC and DC-AC converters. A second chapter is devoted to low voltage single-phase and tri-phase distribution systems and transformers. (J.S.)

  11. Optimization of the telecommunication network of a gas utility; Optimisation d'un reseau de telecommunications d'un operateur gazier

    Energy Technology Data Exchange (ETDEWEB)

    Vinas, Ch.; Abadie, J. [GSO, Gaz Sud-Ouest, 64 - Pau (France)

    2001-07-01

    The information system of Gaz du Sud-Ouest (GSO) utility, which includes the real time systems for the exploitation of the gas transport and distribution system and its management, is based on heterogenous and separated telecommunication infrastructures. A project has been started which aims at optimizing the architecture of this communication network taking into consideration the technical aspects but also the security and costs constraints. (J.S.)

  12. Ixtetreco: recent extensions for the supervision of a power network; Ixtetreco: extensions recentes pour la supervision d`un reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    Despouys, O.

    1998-04-01

    A chronicle model describes a class of evolutions or possible behaviours for a given dynamical system. Ixtetreco uses a reified logics and time constraints to describe these chronicles and algorithms which allow to recognize all instances of these chronicles in a given stream of observations in entry. Ixtetreco is used in several applications for the supervision of complex processes. This paper presents the recent extensions of Ixtetreco which allow to use it in the supervision of power networks. (J.S.) 14 refs.

  13. Towards the prediction of thermal fatigue cracks networks development; Vers la prediction de l'apparition de reseau de fissures en fatigue thermique

    Energy Technology Data Exchange (ETDEWEB)

    Osterstock, St. [CEA Saclay, Dept. des Materiaux pour le Nucleaire (DEN/DANS/DMN/SRMA), 91 - Gif-sur-Yvette (France)

    2008-07-01

    In the framework of the influence of the surface and the structure of materials used in the cooling system of reactor, Depres studied in 2004 at the CEA, the evolution of the microstructure inside the surface grains under a thermal fatigue loading, from dynamic of dislocations calculation. In this context the aim of this study is to bring experimental elements of validation of the numerical results obtained by Depres and to verify if these elements allow the prediction of cracks networks apparition. (A.L.B.)

  14. Strontium Optical Lattice Clock: In Quest of the Ultimate Performance; Horloge a reseau optique au strontium: en quete de la performance ultime

    Energy Technology Data Exchange (ETDEWEB)

    Westergaard, Ph.G.

    2010-10-15

    This thesis presents the latest achievements regarding the Sr optical lattice clock experiment at LNESYRTE, Observatoire de Paris. After having described the general principles for optical lattice clocks and the operation of the clock in question, the emphasis is put on the features that have been added to the experiment since 2007. The most important new elements are an ultra-stable reference cavity for the clock laser, the development of a non-destructive detection technique, and the construction of a second Sr lattice clock. The ultra-stable cavity is constructed from a ULE spacer and fused silica mirrors and has shown a thermal noise floor at 6.5 * 10{sup -16}, placing it among the best in the world. The non-destructive detection is effectuated by a phase measurement of a weak probe beam that traverses the atoms placed in one arm of a Mach-Zender interferometer. The non-destructive aspect enables a recycling of the atoms from cycle to cycle which consequently increases the duty cycle, allowing for an increase of the stability of the clock. With these new tools the frequency stability is expected to be 2.2 * 10{sup -16}/{radical}{tau} for an optimized sequence. The most recent comparisons between the two Sr clocks reach an accuracy level of 10{sup -16} after about 1000 s, and this way we have been able to characterize lattice related frequency shifts with an unprecedented accuracy. The measurements ensure a control of lattice related effects at the 10{sup -18} level even for trap depths as large as 50E{sub r}. (authors)

  15. Civilian Protection in the Eastern DRC: Evaluation of the MONUSCO Peacekeeping Operation

    Science.gov (United States)

    2012-03-01

    1996–2003),” in Reseau des Femmes pour un Developpement Associatif (RFDA), International Alert, 2005. 123 Woudenberg, “You Will Be Punished,” 52...Violence Against Women and Girls During the War in the Democratic Republic of Congo, South Kivu (1996–2003).” Reseau des Femmes pour un

  16. Design and Study of Radioactive Graphite Dust Experimental System in Primary Loop of HTR-10%HTR-10一回路放射性石墨粉尘实验系统设计及研究

    Institute of Scientific and Technical Information of China (English)

    谢锋; 曹建主; 陈志鹏; 董玉杰

    2015-01-01

    在10MW高温气冷堆(HTR‐10)氦净化系统中,设计并建造了用于取样收集一回路放射性石墨粉尘的实验系统。结合国外已有的研究结果,根据HTR‐10氦净化系统的运行参数进行了模拟计算。计算结果表明,该实验系统能有效过滤收集到的放射性石墨粉尘。所设计的取样过滤器便于拆卸和后期测量,可实现对放射性石墨粉尘进行长期系统的研究,给出反应堆不同运行工况下一回路氦净化系统中石墨粉尘及固体裂变核素活度的信息,将为HTR‐10高温气冷堆裂变产物行为研究提供大量重要的实验研究数据。%In the helium purification system of 10 MW high temperature gas‐cooled reac‐tor (HTR‐10) ,an experimental system which could be used to sample the radioactive graphite dust in the primary loop was designed and built .Combined with international research results ,a simulation w as done according to parameters of the helium purifica‐tion system of HTR‐10 .The results indicate that the experimental system can sample the radioactive graphite dust effectively .The sampling filter can be disassembled easily for further measurement ,and used to do a long term systematical study on the radioac‐tive graphite dust .It can provide the informations of the graphite dust and the activity of solid fission nuclides in the primary loop in different operation situations ,and supply large amount of important experimental data to study the behavior of fission products in HTR‐10 .

  17. Research and Application of Packing Density for Pebble Bed in HTR%关于 HTR 球床堆叠密度统计方法的研究及应用

    Institute of Scientific and Technical Information of China (English)

    于福江; 谢菲; 孙喜明

    2015-01-01

    T he pebble bed high temperature gas‐cooled reactor is one of the major types of reactors developed by Chinese nuclear technology .T he statistical analysis for packing density in the pebble bed is an important issue of physical‐thermal calculation and safety analysis .Aimed to this problem ,a new kind of method was set up to solve this prob‐lem .Compared with the traditional lattice‐fill method and the experiment ,its efficiency and accuracy were verified ,while helping to find out the best length of unit in the tradi‐tional lattice‐fill method . This method was used to analyze the boundary effects observed by experiments .%球床式高温气冷堆是目前中国自主研发的主要堆型,对于球床内的球体随机堆叠密度分布的统计分析是高温气冷堆物理‐热工计算和安全分析的重要问题之一。针对这一问题,提出了一种新的堆叠密度分布的统计方法。通过与传统格子填充法计算结构和实验值进行对比,验证了该方法的高效性和准确性,同时帮助传统格子填充法找出最佳的单元尺度,并尝试利用该方法对实验中多次观察到的边界效应进行分析。

  18. Mechanisms of oxide layer formation and destruction on a chromia former nickel base alloy in HTR environment; Mecanismes de formation et de destruction de la couche d'oxyde sur un alliage chrominoformeur en milieu HTR

    Energy Technology Data Exchange (ETDEWEB)

    Rouillard, F

    2007-10-15

    Haynes 230 alloy which contains 22 wt.% chromium could be a promising candidate material for structures and heat exchangers (maximum operating temperature: 850-950 C) in Very High Temperature Reactors (VHTR). The feasibility demonstration involves to valid its corrosion resistance in the reactor specific environment namely impure helium. The alloys surface reactivity was investigated at temperatures between 850 and 1000 C. We especially focused on the influence of different parameters such as concentrations of impurities in the gas phase (carbon monoxide and methane, water vapour/hydrogen ratio), alloy composition (activities of Cr and C, alloying element contents) and temperature. Two main behaviours have been revealed: the formation of a Cr/Mn rich oxide layer at 900 C and its following reduction at higher temperatures. At 900 C, the water vapour is the main oxidizing gas. However in the initial times, the carbon monoxide reacts at the metal/oxide interface which involves a gaseous transport through the scale; CO mainly oxidizes the minor alloying elements aluminium and silicon. Above a critical temperature TA, the carbon in solution in the alloy reduces chromia. To ascribe the scale destruction, a model is proposed based on thermodynamic interfacial data for the alloy, oxide layer morphology and carbon monoxide partial pressure in helium; the model is then validated regarding experimental results and observations. (author)

  19. Contribution to the experimental study of the critical scattering of cold neutrons in iron; Contriiution a l'etude experimentale de la diffusion critique des neutrons froids par le fer

    Energy Technology Data Exchange (ETDEWEB)

    Konstantinovic, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-03-15

    The aim of the present work is a study of magnetic fluctuations which are produced in iron in the neighbourhood of the Curie temperature, by neutron scattering. We start by briefly recalling the theory of scattering of neutrons by magnetic substances and Landau's theory of second order phase transitions which enables one to derive the magnetic cross section near the Curie temperature. Following this is a description of the experimental apparatus after which we present the experimental results. The analysis of the results confirms the four-third law obeyed by the magnetic susceptibility near the Curie point, predicted by recent theories based on the Heisenberg model. However, the analysis reveals a non-zero relaxation time for the magnetic fluctuations at the Curie point, which is in disagreement with theoretical conclusions. (author) [French] L'objet du present travail est l'etude des fluctuations d'aimantation qui prennent naissance dans le fer au voisinage de sa temperature de Curie par la diffusion des neutrons. Nous commencons par rappeler brievement les generalites sur la diffusion des neutrons par les substances magnetiques et la theorie de Landau des transitions de phase du second ordre qui permet de deriver une expression de la section efficace magnetique pres de la temperature de Curie. Ensuite, apres la description du dispositif experimental, nous presentons les resultats experimentaux. L'analyse de ces resultats confirme les theories recentes suivant le modele d'Heisenberg en ce qui concerne la 'loi en 4/3' de la susceptibilite magnetique au voisinage du point de Curie; mais par ailleurs elle revele l'existence d'un temps de relaxation des fluctuations d'aimantation non nul en ce point, ce qui est en desaccord avec les previsions theoriques actuelles. (auteur)

  20. Combined heating and cooling installation in a new building in Quartino, Switzerland; Installation de production combinee de chaud et de froid du nouveau batiment de Grunenfelder SA a Quartino

    Energy Technology Data Exchange (ETDEWEB)

    Curti, V.

    2004-07-01

    This final report for the Swiss Federal Office of Energy (SFOE) describes a multi-temperature heating and cooling system implemented at the Gruenenfelder SA, a food wholesaler in southern Switzerland. The system provides space heating, hot water, warm water for the cleaning of vehicles, refrigeration for over 2,000 square meters of deep-freeze storage as well as refrigerated and lightly-cooled storage for goods on pallets. The cooling temperatures required range from -28 {sup o}C to +6 {sup o}C. Further, heat is provided to ensure that no trouble is caused by permafrost. The results of measurements made on the energy balances over a period of around 1.5 years are presented and discussed. The heat pump, with a capacity of 305 kW for cooling in summer and 382 kW for heating in winter is described, as is the refrigeration group with a capacity of 447 kW. The results show that this type of integrated system can reduce heat rejection into the atmosphere and can form the basis of further projects of this type.

  1. Etude théorique et expérimentale d'échangeurs géothermiques hélicoïdaux : Production de chaud et de froid par pompe à chaleur, et dimensionnement d'installations

    OpenAIRE

    2013-01-01

    This thesis consists in a study of geothermal helical heat exchangers buried in the close underground. These objects belong to the compact exchangers kind and may be used instead of borehole heat exchanger or geothermal horizontal exchangers to cope with the needs in heating and cooling buildings with a heat pump. A theoretical and experimental study of these exchangers leads to numerical and analytical models which can be used to estimate the temperatures in the underground and in the exchan...

  2. Aggressive Ewing's sarcoma appearing as a cold lesion on bone scan; Sarcome d'Ewing agressif apparaissant comme une lesion froide sur la scintigraphie osseuse

    Energy Technology Data Exchange (ETDEWEB)

    Chatti, K.; Guezguez, M.; Maha Ben Fredj, M.; Sfar, R.; Essabbah, H. [Hopital Universitaire de Sahloul, Dept. de Medecine Nucleaire, Sahloul (Tunisia); Mtaoumi, M. [Hopital Universitaire de Sahloul, Dept. d' Orthopedie, Sousse (Tunisia); Chatti, K. [Faculte de Medecine de Monastir, Lab. de Biophysique, Monastir (Tunisia)

    2009-10-15

    Ewing's sarcoma classically presents as a hot spot on bone scan as a result of increased vascularity of the tumor and new bone formation. Purpose We report and analyze an uncommon pattern of a 'cold' lesion in Ewing's sarcoma on bone scan and its pathophysiologic significance. Case report A 15-year-old boy complaining of thigh pain. CT scan evoked Ewing's sarcoma or osteitis. MRI evoked chronic osteitis. Scintigraphy showed a fairly intense and heterogeneous uptake on the femoral lesion and no abnormal uptake elsewhere. Biopsy showed none pathologic pattern. Three months later, a second biopsy concluded to Ewing's sarcoma. Bone scan showed a larger lesion with peripheral intense uptake centered by enlarged 'cold' area in the left femoral diaphysis and no evident bone metastasis. The patient underwent chemotherapy and surgery. Three months later, bone scan showed extensive skeletal metastasis. Conclusion Ewing's sarcoma appears usually as an intense lesion on bone scan. Nevertheless, decreased radiopharmaceutical uptake or 'cold' lesion may be seen in aggressive Ewing's sarcoma with lytic tumor, growth of which is very rapid and bony reaction is minimal. (authors)

  3. Solar process for cold production at low temperature (-28 deg) by solid-gas sorption; Procede solaire de production de froid basse temperature (-28 deg) par sorption solide-gaz

    Energy Technology Data Exchange (ETDEWEB)

    Le Pierres, N.

    2005-09-15

    The aim of this work is the design and experimentation of a thermochemical process for cold production purpose at -28 deg. C using low grade heat (70 deg. C) supplied by flat plate solar collectors. An exergetic analysis of the thermo-chemical dipole and of ideal thermodynamic cycles led to the definition of an original solar process. This process involves two cascaded cycles functioning in parallel. The cycle is discontinuous and presents a day phase of heating and regeneration and a night phase of cold production. A dynamic simulation allowed the study of its functioning depending on the weather conditions and on the dimensions of the process. A prototype covering the needs of a cold chamber loosing 40 W of cold continuously was built and tested in real conditions in Perpignan. It demonstrated the feasibility of this innovative concept and validated the hypothesis used to develop the model. A study of the process functioning was lead by simulation in different meteorological conditions and for different heat sources (solar, geothermal or industrial waste). It showed the potentialities of the concept. (author)

  4. Chromium metal plating followed by rinsing and by regeneration of rinse water on ion exchange resins with recycling; Chromage de pieces suivi de rincages, avec regeneration du dernier bain de rincage froid sur resines echangeuses d`ions et recyclage

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    This audit presents a modification of the rinsing stages of the conventional chromium plating technology. After chromium plating the parts are rinsed. The first rinsing bath which is high in chromic acid, is recycled and the last cold rinsing bath is regenerated on ion-exchange resins and recycled. In the conventional process, the last cold rinsing bath is discarded.

  5. Optimisation des paramètres d'extraction à froid de l'huile d'Azadirachta indica A. Juss et effets sur quelques caractéristiques chimiques de l'huile extraite

    Directory of Open Access Journals (Sweden)

    Nitièma-Yefanova, S.

    2012-01-01

    Full Text Available Optimization of the parameters of cold extraction of the oil of Azadirachta indica A. Juss and effects on some chemical parameters of the extracted oil. Azadirachta indicaA.Juss (neem is a tropical tree, popular for its multiple possible uses. The oil extracted from neem kernels is used in cosmetics, as medicines, as insecticides and for energy needs (biofuel. The oldest oil extraction method is cold pressing. However, the optimal parameters of extraction and the quality of extracted oil are poorly known. The purpose of this research was to identify the optimal extracting parameters of neem oil by cold pressing and to characterize the extracted oil. The results showed that at 25 °C, the optimum yield by pressing of whole kernels was obtained with a pressure of 30.4 MPa ± 4.1 and a maximum degree of filling of the pressing cage. We showed that maintaining this pressure for 6 min could lead to an extraction rate of 40.1% ± 1.1. The oil yield increased with the speed of kernel compression. Reducing the particle size had a positive effect on oil yield when the cage load did not exceed half of the cage capacity. The best oil yield of 40.3% ± 0.0 for ground kernels was obtained with a pressure of 33.7 MPa ± 2.9 and a cage filled in the quarter of its capacity. The content levels of water, iron, copper and phosphorus in the oil extracted by pressing were respectively 0.14%; 11.8; 0.1 and 17 ppm. These characteristics indicate, inter alia, that neem oil obtained by cold pressing is of appropriate quality for use in biodiesel production.

  6. Numerical modelling of coupled phenomena within molten glass heated by induction and mechanically stirred; Modelisation numerique de phenomenes couples dans des bains de verre brasses mecaniquement et elabores en creuset froid inductif

    Energy Technology Data Exchange (ETDEWEB)

    Jacoutot, L

    2006-11-15

    This study reports on a new vitrification process developed by the French Atomic Energy Commission (CEA, Marcoule). This process is used for the treatment of high activity nuclear waste. It is characterized by the cooling of all the metal walls and by currents directly induced inside the molten glass. In addition, a mechanical stirring device is used to homogenize the molten glass. The goal of this study is to develop numerical tools to understand phenomena which take place within the bath and which involve thermal, hydrodynamic and electromagnetic aspects. The numerical studies are validated using experimental results obtained from pilot vitrification facilities. (author)

  7. Thermo-hydrodynamic and inductive modelling of a glass melt elaborated in cold inductive crucible; Modelisation numerique thermo-hydrodynamique et inductive d'une fonte verriere elaboree en creuset froid inductif

    Energy Technology Data Exchange (ETDEWEB)

    Sauvage, E

    2009-11-15

    Within the context of a search for a new vitrification process for nuclear wastes with a replacement of the presently used metallic pot by an inductive cold crucible, this research thesis deals with the numerical modelling of this technology. After having recalled the interest of nuclear waste vitrification, this report presents the new process based on the use of a cold crucible, describing principles and objectives of this method, and the characteristic physical phenomena associated with the flow and the thermodynamics of the glassy melt in such a crucible. It also recalls and comments the existing works on modelling. The main objective of this research is then to demonstrate the feasibility of 3D thermo-hydraulic and inductive simulations. He describes and analyses the glass physical properties (electrical properties, viscosity, thermal properties), the electromagnetic, hydrodynamic and thermal phenomena. He presents in detail the bubbling mixing modelling, reports 3D induction and fluid mechanical coupling calculations, and specific thermal investigations (radiating transfers, thermal limit conditions)

  8. Detailed study of the heat sink of Saint-Laurent-des-Eaux nuclear plant; Etude detaillee de la source froide de la centrale de Saint-Laurent des Eaux

    Energy Technology Data Exchange (ETDEWEB)

    Audrerie, Y

    1997-12-31

    This work is dedicated to a detailed study of the reliability of the heat sink of the nuclear plant which is situated on the banks of the Loire river. In the first part the requirements the heat sink has to comply with are reviewed, in the second part the various operating modes, the different procedures established to cope with the hazards of the river: icing up, frost, overflow, lowest water, choking are reported. This analysis highlights the good compliance of the heat sink with the specifications but some improvements about periodic testing are proposed. (A.C.)

  9. 30% of abatement of coldness needs + free re-heating at the skating rink of Chalons-en-Champagne; 30% de reduction des besoins de froid + rechauffage gratuit a la patinoire de Chalon-en-Champagne

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2005-12-01

    The skating rink of Chalons-en-Champagne (France) is equipped with a air treatment system based on the use of a sensible energy transfer battery which reduces by 30% the coldness needs for dehumidification. This process uses part of the heat released by the condensers to generate ice. The result is a 30% reduction of the installed power and a 40% reduction of energy consumptions with respect to standard air treatment solutions. (J.S.)

  10. Ultracold lithium-6 atoms in the BEC-BCS crossover: experiments and the construction of a new apparatus; Atomes de lithium-6 ultra froids dans la transition BEC-BCS: experiences et construction d'un montage experimental

    Energy Technology Data Exchange (ETDEWEB)

    Teichmann, M

    2007-09-15

    We use a fermionic gas of Lithium-6 as a model system to study superfluidity. The limiting cases of superfluidity are Bose-Einstein condensation (BEC) and superconductivity, described by the theory by Bardeen, Cooper and Schrieffer (BCS). In Lithium-6 gases, we can explore the whole range between the two cases, known as the BEC-BCS crossover, using a Feshbach resonance. We study the change of the momentum distribution of the gas in this cross-over and compare to theoretical models. We also investigate the hydrodynamic expansion, characteristic for a superfluid gas. We observe a sudden change of the ellipticity of the gas close to the transition to the superfluid phase. Moreover, we localized heteronuclear Feshbach resonances between {sup 6}Li and {sup 7}Li. We are currently constructing a second generation of the experimental setup. An new laser system, based on high power laser diodes, was developed. Changes in the vacuum chamber, including a complete reconstruction of the Zeeman slower, have increased the atomic flux, allowing us to increase the repetition rate of our experiment. Modifications of the geometry of the magnetic traps lead to a higher number of trapped atoms. (author)

  11. Final Report on Utilization of TRU TRISO Fuel as Applied to HTR Systems Part II: Prismatic Reactor Cross Section Generation

    Energy Technology Data Exchange (ETDEWEB)

    Vincent Descotes

    2011-03-01

    The deep-burn prismatic high temperature reactor is made up of an annular core loaded with transuranic isotopes and surrounded in the center and in the periphery by reflector blocks in graphite. This disposition creates challenges for the neutronics compared to usual light water reactor calculation schemes. The longer mean free path of neutrons in graphite affects the neutron spectrum deep inside the blocks located next to the reflector. The neutron thermalisation in the graphite leads to two characteristic fission peaks at the inner and outer interfaces as a result of the increased thermal flux seen in those assemblies. Spectral changes are seen at least on half of the fuel blocks adjacent to the reflector. This spectral effect of the reflector may prevent us from successfully using the two step scheme -lattice then core calculation- typically used for light water reactors. We have been studying the core without control mechanisms to provide input for the development of a complete calculation scheme. To correct the spectrum at the lattice level, we have tried to generate cross-sections from supercell calculations at the lattice level, thus taking into account part of the graphite surrounding the blocks of interest for generating the homogenised cross-sections for the full-core calculation. This one has been done with 2 to 295 groups to assess if increasing the number of groups leads to more accurate results. A comparison with a classical single block model has been done. Both paths were compared to a reference calculation done with MCNP. It is concluded that the agreement with MCNP is better with supercells, but that the single block model remains quite close if enough groups are kept for the core calculation. 26 groups seems to be a good compromise between time and accu- racy. However, some trials with depletion have shown huge variations of the isotopic composition across a block next to the reflector. It may imply that at least an in- core depletion for the number density calculation may be necessary in the complete calculation scheme.

  12. Association of the 5HTR2A gene with suicidal behavior: CASE-control study and updated meta-analysis

    Directory of Open Access Journals (Sweden)

    González-Castro Thelma Beatriz

    2013-01-01

    Full Text Available Abstract Background The polymorphism rs6313 (T102C has been associated with suicidal behavior in case–control and meta-analysis studies, but results and conclusions remain controversial. The aim of the present study was to examine the association between T102C with suicidal behavior in a case–control study and, to assess the combined evidence – this case–control study and available data from other related studies – we carried out a meta-analysis. Methods We conducted a case–control study that included 161 patients with suicide attempts and 244 controls; we then performed a meta-analysis. The following models were evaluated in the meta-analysis: A C allele vs T allele; B T allele vs C allele; C Caucasian population, D Asian population, and E suicide attempters with schizophrenia. Results We found an association between attempted suicide and control participants for genotype (χ2=6.28, p=0.04, df=2 and allele (χ2=6.17, p=0.01, df=1, OR 1.48 95% IC: 1.08-2.03 frequencies in the case–control study. The meta-analysis, comprising 23 association studies (including the present one, showed that the rs6313 polymorphism is not associated with suicidal behavior for the following comparisons:T allele vs C allele (OR: 1.03; 95% CI 0.93-1.13; p(Z=0.44; C allele vs T allele: (OR:0.99; 95% CI: 0.90-1.08; p(Z=0.22; Caucasians (OR:1.09; 95% CI: 0.96-1.23, and Asians (OR:0.96; 95% CI: 0.84-1.09. Conclusion Our results showed association between the rs6313 (T102C polymorphism and suicidal behavior in the case–control study. However, the meta-analysis showed no evidence of association. Therefore, more studies are necessary to determine conclusively an association between T102C and suicidal behavior.

  13. Inhibitors of Helicobacter pylori Protease HtrA Found by ‘Virtual Ligand’ Screening Combat Bacterial Invasion of Epithelia

    OpenAIRE

    Martin Löwer; Tim Geppert; Petra Schneider; Benjamin Hoy; Silja Wessler; Gisbert Schneider

    2011-01-01

    BACKGROUND: The human pathogen Helicobacter pylori (H. pylori) is a main cause for gastric inflammation and cancer. Increasing bacterial resistance against antibiotics demands for innovative strategies for therapeutic intervention. METHODOLOGY/PRINCIPAL FINDINGS: We present a method for structure-based virtual screening that is based on the comprehensive prediction of ligand binding sites on a protein model and automated construction of a ligand-receptor interaction map. Pharmacophoric featur...

  14. HTR-2014 Paper Comparison of fission product release predictions using PARFUME with results from the AGR-1 irradiation experiment

    Energy Technology Data Exchange (ETDEWEB)

    Blaise Collin

    2001-10-01

    The PARFUME (PARticle FUel ModEl) code was used to predict fission product release from tristructural isotropic (TRISO) coated fuel particles and compacts during the first irradiation experiment (AGR-1) of the Advanced Gas Reactor Fuel Development and Qualification program. The PARFUME model for the AGR-1 experiment used the fuel compact volume average temperature for each of the 620 days of irradiation to calculate the release of fission products silver, cesium, and strontium from a representative particle for a select number of AGR-1 compacts. Post-irradiation examination (PIE) measurements provided data on release of fission products from fuel compacts and fuel particles, and retention of fission products in the compacts outside of the SiC layer. PARFUME-predicted fractional release of these fission products was determined and compared to the PIE measurements. Results show an overall over-prediction of the fractional release of cesium by PARFUME. For particles with failed silicon carbide (SiC) layers, the over-prediction is by a factor of about two, corresponding to an over-estimation of the diffusivity in uranium oxycarbide (UCO) by a factor of about 100. For intact particles, whose release is much lower, the over-prediction is by an average of about an order of magnitude, which could additionally be attributed to an over-estimated diffusivity in SiC by about 30%. The release of strontium from intact particles is also over-estimated by PARFUME, which also points towards an over-estimated diffusivity of strontium in either SiC or UCO, or possibly both. The measured strontium fractional release from intact particles varied considerably from compact to compact, making it difficult to assess the effective over-estimation of the diffusivities. Furthermore, the release of strontium from particles with failed SiC is difficult to observe experimentally due to the release from intact particles, preventing any conclusions to be made on the accuracy or validity of the PARFUME predictions and the modeled diffusivity of strontium in UCO. In the case of silver, the comparisons between PARFUME and PIE are better than for cesium and strontium. They show a trend of over-prediction at low burnup and under-prediction at high burnup. PARFUME has limitations in the modeling of the temporal and spatial distributions of the temperature and burnup across the compacts, which affects the accuracy of its predictions. Nevertheless, the comparisons lie in the same order of magnitude.

  15. Effects of thermal treatment on the mechanical integrity of silicon carbide in HTR fuel up to 2200 °C

    Science.gov (United States)

    Rohbeck, Nadia; Xiao, Ping

    2014-08-01

    Achieving inherent safety of the High Temperature Reactor relies on the exceptional performance of its fuel. The design foresees complete encapsulation of all fissionable material by layers of carbon and silicon carbide (SiC) forming the tristructural-isotropic fuel particle. Its mechanical integrity and ability to fully retain fission products even in the event of an accident is a vital safety concern. The present study investigates the effect of post-deposition annealing on the SiC coating at design-based accident temperatures and beyond. Therefore, samples of simulated fuel have been fabricated by fluidized bed chemical vapour deposition and thermally treated in inert atmosphere up to 2200 °C. Nanoindentation and crush test measurements showed only minor reductions of elastic modulus and fracture strength up to 2000 °C. Substantial weight loss and crystal growth were observed at annealing temperatures of 2100 °C and above. Raman spectroscopy identified the formation of a multi-layered graphene film covering the SiC grains after annealing and scanning electron microscopy revealed significant porosity formation within the coating from 1800 °C onwards. These observations were attributed towards an evaporation-precipitation mechanism of SiC at very elevated temperatures that only slightly diminishes the hardness, elastic modulus or fracture strength, but might still be problematic in respect to fission product retention of the SiC layer.

  16. HTR1A a novel type 1 diabetes susceptibility gene on chromosome 5p13-q13

    DEFF Research Database (Denmark)

    Asad, Samina; Nikamo, Pernilla; Gyllenberg, Alexandra;

    2012-01-01

    We have previously performed a genome-wide linkage study in Scandinavian Type 1 diabetes (T1D) families. In the Swedish families, we detected suggestive linkage (LOD≤2.2) to the chromosome 5p13-q13 region. The aim of our study was to investigate the linked region in search for possible T1D...... susceptibility genes....

  17. A safety re-evaluation of the AVR pebble bed reactor operation and its consequences for future HTR concepts

    Energy Technology Data Exchange (ETDEWEB)

    Moormann, R.

    2008-06-15

    The AVR pebble bed reactor (46 MW{sub th}) was operated 1967-88 at coolant outlet temperatures up to 990 C. A principle difference of pebble bed HTRs as AVR to conventional reactors is the continuous movement of fuel element pebbles through the core which complicates thermohydraulic, nuclear and safety estimations. Also because of a lack of other experience AVR operation is still a relevant basis for future pebble bed HTRs and thus requires careful examination. This paper deals mainly with some insufficiently published unresolved safety problems of AVR operation and of pebble bed HTRs but skips the widely known advantageous features of pebble bed HTRs. The AVR primary circuit is heavily contaminated with metallic fission products (Sr-90, Cs-137) which create problems in current dismantling. The amount of this contamination is not exactly known, but the evaluation of fission product deposition experiments indicates that the end of life contamination reached several percent of a single core inventory, which is some orders of magnitude more than precalculated and far more than in large LWRs. A major fraction of this contamination is bound on graphitic dust and thus partly mobile in depressurization accidents, which has to be considered in safety analyses of future reactors. A re-evaluation of the AVR contamination is performed here in order to quantify consequences for future HTRs (400 MW{sub th}). It leads to the conclusion that the AVR contamination was mainly caused by inadmissible high core temperatures, increasing fission product release rates, and not - as presumed in the past - by inadequate fuel quality only. The high AVR core temperatures were detected not earlier than one year before final AVR shut-down, because a pebble bed core cannot yet be equipped with instruments. The maximum core temperatures are still unknown but were more than 200 K higher than calculated. Further, azimuthal temperature differences at the active core margin of up to 200 K were observed, probably due to a power asymmetry. Unpredictable hot gas currents with temperatures >1100 C, which may have harmed the steam generator, were measured in the top reflector range. After detection of the inadmissible core temperatures, the AVR hot gas temperatures were strongly reduced for safety reasons. Thus a safe and reliable AVR operation at high coolant temperatures, which is taken as a foundation of the pebble bed VHTR development in Generation IV, was not conform with reality. Despite of remarkable effort spent in this problem the high core temperatures, the power asymmetry and the hot gas currents are not yet understood. It remains uncertain whether convincing explanations can be found on basis of the poor AVR data and whether pebble bed specific effects are acting. Respective examinations are however ongoing. Reliable predictions of pebble bed temperatures are at present not yet possible. (orig.)

  18. Combined HTR2C-LEP Genotype as a Determinant of Obesity in Patients Using Antipsychotic Medication

    NARCIS (Netherlands)

    Gregoor, Jochem G.; Mulder, Hans; Cohen, Dan; van Megen, Harold J. G. M.; Egberts, Toine C. G.; Heerdink, Eibert R.; van der Weide, Jan

    2010-01-01

    Obesity is one of the most serious common somatic adverse effects of atypical antipsychotic agents. Genetic factors partly determine the individual patient's risk of developing obesity during treatment. As weight-regulating mechanisms, such as the leptinergic and serotonergic system, may be interdep

  19. Neuroprotective effects of HTR1A antagonist WAY-100635 on scopolamine-induced delirium in rats and underlying molecular mechanisms.

    Science.gov (United States)

    Qiu, Yimin; Chen, Dongmei; Huang, Xiaojing; Huang, Lina; Tang, Liang; Jiang, Jihong; Chen, Lianhua; Li, Shitong

    2016-10-19

    Limited surveys have assessed the performance of 5-hydroxytreptamine receptor 1A and its antagonist WAY-100635 in pharmacological manipulations targeting delirium therapies. The purpose of this paper was to assess the central pharmacological activity of WAY-100635 in a rat model of scopolamine-induced delirium and its underlying mechanism. A delirium rat model was established by intraperitoneal injection of scopolamine and behavioral changes evaluated through open field and elevated plus maze experiments. Concentrations of monoamines in the hippocampus and amygdalae were detected by high performance liquid chromatography. The effect of WAY-100635 on the recovery of rats from delirium was assessed by stereotactic injection of WAY-100635 and its mechanism of action determined by measuring mRNA and protein expression via real time PCR and western blotting methods. The total distance and the number of crossing and rearing in the elevated plus maze test and the time spent in the light compartment in the dark/light test of scopolamine-treated rats were significantly increased while the percentage of time spent in the open arms was decreased, showing the validity of the established delirium rat model. The measurement of the concentrations of noradrenaline, 3,4-dihydroxyphenylacetic acid, the homovanillic acid, 5-hydroxy-3-indoleacetic acid and serotonin concentrations in the cerebrospinal fluid (CSF) of scopolamine-induced delirium rats were significantly increased. The intra-hippocampus and intra-BLA injections of WAY-100635 improved the delirium-like behavior of rats by significantly reducing the expression of NLRP3 inflammasome and the release of IL1-β and IL8 into CSF. Taken together, these findings indicate that WAY-100635 may exert a therapeutic effect on post-operative delirium by controlling neurotransmission as well as suppressing neuroinflammation in the central nervous system.

  20. STUDI DESAIN DOWN SCALE TERAS REAKTOR DAN BAHAN BAKAR PLTN JENIS PEBBLE BED MODULAR REACTOR – HTR 100 MWe

    Directory of Open Access Journals (Sweden)

    Slamet Parmanto

    2015-04-01

    Full Text Available Telah dilakukan penelitian terhadap teras reaktor Pebble Bed Modular Reactor (PBMR dengan daya 100 Mwe berbahan bakar UO2. Reaktor ini menggunakan moderator grafit dan helium sebagai pendingin. Studi down scale dilakukan tanpa mengubah geometri teras maupun geometri bahan bakar. Parameter yang dianalisis adalah kritikalitas teras, reaktivitas lebih, koefisien reaktivitas temperatur bahan bakar, moderator dan pendingin serta nilai ekonomis bahan bakar. Dari penelitian ini diharapkan diperoleh desain bahan bakar yang bernilai ekonomis dan memiliki fitur keselamatan melekat. Penelitian dilakukan dengan menggunakan program SRAC 2003. Hasil yang diperoleh adalah desain bahan bakar UO2 berbentuk pebble dengan pengkayaan 10% U235 dan 90 ppm racun dapat bakar Gd2O3. Nilai faktor multipilkasi effektif keff pada beginning of life (BOL adalah 1,01115 dan menjadi 1,00588 setelah 2658 hari operasi reaktor (EOL. Koefisien reaktivitas temperatur total diperoleh sebesar - 3,25900E-05 ∆k/k/K saat BOL dan -1,10615E-04 ∆k/k/K saat end of life (EOL. Reaktor ini memenuhi karakteristik keselamatan melekat ditandai dengan nilai koefisien reaktivitas temperatur yang negatif. Kata kunci: PBMR, desain bahan bakar, faktor multipilkasi effektif, reaktivitas lebih, koefisien reaktivitas temperatur.   Research of Pebble Bed Modular Reactor (PBMR 100 MWe which used UO2 fuel has been done. This reactor uses graphite as moderator and helium as coolant. Down scale studies performed without changing the core and fuel geometry. The parameter being analyzed were core criticality, excess reactivity, fuel, moderator, coolant temperature reactivity coefficient, and fuel economy. This research is expected to obtain the design that has fuel economy and inherent safety features. In this research, we have employed SRAC 2003 code. The calculation show that the UO2 pebble fuel design with 10% enrichment of U235 and 90 ppm burnable poison of Gd2O3 results in the effective multiplication factor (keff value of 1,01115 at beginning of life (BOL and become 1,00588 after 2658 days of reactor operation. The core temperature reactivity coefficient is -3.25900E-05 ∆k/k/K and -1,100615E-04 ∆k/k/K at BOL and end of life (EOL, respectively. The reactor is in compliance with inherent safety characteristics indicated by the value of a negative temperature reactivity coefficient. Keywords: PBMR, fuel design, effective multiplication factor, excess reactivity, temperature reactivity coefficient.

  1. Genome-wide association studies identify CHRNA5/3 and HTR4 in the development of airflow obstruction

    NARCIS (Netherlands)

    Wilk, Jemma B; Shrine, Nick R G; Loehr, Laura R; Zhao, Jing Hua; Manichaikul, Ani; Lopez, Lorna M; Smith, Albert Vernon; Heckbert, Susan R; Smolonska, Joanna; Tang, Wenbo; Loth, Daan W; Curjuric, Ivan; Hui, Jennie; Cho, Michael H; Latourelle, Jeanne C; Henry, Amanda P; Aldrich, Melinda; Bakke, Per; Beaty, Terri H; Bentley, Amy R; Borecki, Ingrid B; Brusselle, Guy G; Burkart, Kristin M; Chen, Ting-hsu; Couper, David; Crapo, James D; Davies, Gail; Dupuis, Josée; Franceschini, Nora; Gulsvik, Amund; Hancock, Dana B; Harris, Tamara B; Hofman, Albert; Imboden, Medea; James, Alan L; Khaw, Kay-Tee; Lahousse, Lies; Launer, Lenore J; Litonjua, Augusto; Liu, Yongmei; Lohman, Kurt K; Lomas, David A; Lumley, Thomas; Marciante, Kristin D; McArdle, Wendy L; Meibohm, Bernd; Morrison, Alanna C; Musk, Arthur W; Myers, Richard H; North, Kari E; Postma, Dirkje S; Psaty, Bruce M; Rich, Stephen S; Rivadeneira, Fernando; Rochat, Thierry; Rotter, Jerome I; Artigas, María Soler; Starr, John M; Uitterlinden, André G; Wareham, Nicholas J; Wijmenga, Cisca; Zanen, Pieter; Province, Michael A; Silverman, Edwin K; Deary, Ian J; Palmer, Lyle J; Cassano, Patricia A; Gudnason, Vilmundur; Barr, R Graham; Loos, Ruth J F; Strachan, David P; London, Stephanie J; Boezen, Hendrika; Probst-Hensch, Nicole; Gharib, Sina A; Hall, Ian P; O'Connor, George T; Tobin, Martin D; Stricker, Bruno H

    2012-01-01

    RATIONALE: Genome-wide association studies (GWAS) have identified loci influencing lung function, but fewer genes influencing chronic obstructive pulmonary disease (COPD) are known. OBJECTIVES: Perform meta-analyses of GWAS for airflow obstruction, a key pathophysiologic characteristic of COPD asses

  2. Recombinant outer membrane vesicles carrying Chlamydia muridarum HtrA induce antibodies that neutralize chlamydial infection in vitro

    OpenAIRE

    Bartolini, Erika; Ianni, Elvira; Frigimelica, Elisabetta; Petracca, Roberto; Galli, Giuliano; Berlanda Scorza, Francesco; Norais, Nathalie; Laera, Donatello; Giusti, Fabiola; Pierleoni, Andrea; Donati, Manuela; Cevenini, Roberto; Finco, Oretta; Grandi, Guido; Grifantini, Renata

    2013-01-01

    Background: Outer membrane vesicles (OMVs) are spheroid particles released by all Gram-negative bacteria as a result of the budding out of the outer membrane. Since they carry many of the bacterial surface-associated proteins and feature a potent built-in adjuvanticity, OMVs are being utilized as vaccines, some of which commercially available. Recently, methods for manipulating the protein content of OMVs have been proposed, thus making OMVs a promising platform for recombinant, multivalent v...

  3. The role of dopamine D3, 5-HT2A and 5-HT2C receptor variants as pharmacogenetic determinants in tardive dyskinesia in African-Caribbean patients under chronic antipsychotic treatment

    NARCIS (Netherlands)

    Wilffert, B.; Al Hadithy, A.F.; Sing, V.J.; Matroos, G.; Hoek, H.W.; van Os, J.; Bruggeman, R.; Brouwers, J.R.; van Harten, P.N.

    2009-01-01

    Abstract Tardive dyskinesia (TD) is associated with polymorphisms of the dopamine D3, serotonin 2A and 2C receptors (DRD3, HTR2A and HTR2C, respectively). This study investigated the possible relationship between TD and the polymorphisms Ser9Gly (DRD3), 102T>C (HTR2A), -1438G>A(HTR2A) and Cys23Ser (

  4. Preliminary study on field buses for the control system of the high voltage of the ATLAS hadronic calorimeter; Etude preliminaire d`un reseau de terrain pour le systeme de controle des hautes tensions du calorimetre hadronique d`Atlas

    Energy Technology Data Exchange (ETDEWEB)

    Drevet, F.; Chadelas, R.; Montarou, G.

    1996-12-31

    We present here after a preliminary study on field buses for the control system of the high voltage of the photomultipliers of the TILECAL calorimeter. After some generalities, different commercial buses are reviewed (CAN, ARCET, WorldFIP, Profibus and LonWorks). The Profibus and LonWorks solution are more extensively studies as a possible solution for the high voltage system of the TILE hadronic calorimeter. (authors).

  5. The national network of measurements of radioactivity in the environment. Management report - 2010; Reseau national de mesures de la radioactivite de l'environnement. Rapport de gestion - Annee 2010

    Energy Technology Data Exchange (ETDEWEB)

    Leprieur, Fabrice; Chaptal-Gradoz, Nathalie [Institut de radioprotection et de surete nucleaire - IRSN, Direction de l' environnement et de l' intervention, Service d' etude et de surveillance de la radioactivite dans l' environnement, DEI/SESURE/LVRE, BP35, 78116 Le Vesinet (France); Wyckaert, Laure; Guldner, Bruno [Institut de radioprotection et de surete nucleaire - IRSN, Direction de l' environnement et de l' intervention, Groupe informatique et scientifique, BP35, 78116 Le Vesinet (France); Jaunet, Pierrick; Levelut, Marie-Noelle [Autorite de surete nucleaire - ASN, Direction de l' environnement et des situations d' urgence, 6, place du Colonel Bourgoin, 75572 Paris Cedex 12 (France)

    2010-07-01

    This report presents the objectives and challenges of the French national network for the measurement of radioactivity in the environment, its legal and regulatory context, its operation, its actors (ASN, IRSN and other actors). It proposes the moral report on the steering committee and work-groups. It describes the development of the information system: main stages, synthetic description, process from data transmission to edition on Internet sites, exploitation of the public Internet site, of the requester internet site, of hosting platforms, harmonization of transmitted data, planning for 2011. It presents the exploitation assessment for 2011: technical support activities, interactions between the IRSN and the national network information system host, and so on. The last part deals with communication and publication activities

  6. Thematic outlook. Technical outlook for the fuel-cell research network (PACo). December 7, 2001 update, no. 3; Veille thematique. La veille technique pour le reseau PACo. Actualisation du 7 decembre 2001, no. 3

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-12-01

    This report brings together a compilation of abstracts of articles about some recent research works carried out in the domain of fuel cells, hydrogen production and hydrogen storage, and of renewable energies in general: studies of the humidification of PEMFCs; chemical engineering in the automotive industry; environmental impact of the manufacturing of tubular and planar SOFCs; fullerenes for portable fuel cells; hydrogen fuel produced from the gasification of solid municipal fuels; required electric power for a large-scale production of hydrogen for vehicles; hydrogen produced from methane decomposition: an application for the decarbonization of fossil fuels; production of synthesis gas from ethanol vapo-reforming; electrochemical characterization of the hydrogen adsorption capacity of carbon nano-tubes prepared by chemical vapor deposition (CVD), electric arc and laser ablation techniques; hydrogen storage in ultrasound wave processed carbonized materials; hydrogen storage in fuel cell-powered vehicles; year 2000 status of renewable energies in France; renewable energy sources: too insufficient efforts made in the UK; wind turbine in the country of wind mills (Netherlands); a research institute for wind energy in the Netherlands; feasibility study of underwater CO{sub 2} storage. (J.S.)

  7. Thematic survey: the technical survey for the PACo network. Actualization of the 30 september 2003, no.8; Veille thematique: la veille technique pour le reseau PACo. Actualisation du 30 septembre 2002, no.8

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    This document provides the thematic survey concerning the hydrogen fuel cell, realized on september 2003. The papers published during this period deal with the hydrogen fuel cell (low power stationary, mobile and handling applications, components, vehicles), the hydrogen production (the ethanol reforming), hydrogen storage (materials activation), safety, regulation and standards, carbon dioxide emissions decrease and fuel production. For each purposes abstracts are provided. (A.L.B.)

  8. Assessment, modelization and analysis of {sup 106} Ru experimental transfers through a freshwater trophic system; Evaluation, modelisation et analyse des transferts experimentaux du {sup 106}Ru au sein d`un reseau trophique d`eau douce

    Energy Technology Data Exchange (ETDEWEB)

    Vray, F.

    1994-11-24

    Experiments are carried out in order to study {sup 106} RU transfers through a freshwater ecosystem including 2 abiotic compartments (water and sediment) and 3 trophic levels (10 species). Experimental results are expressed mathematically so as they can be included into a global model which is then tested in two different situations. The comparison of the available data concerning the in situ measured concentrations to the corresponding calculated ones validates the whole procedure. Analysis of the so validated results lightens ruthenium distribution process in the environment. The rare detection of this radionuclide in organisms living in areas contaminated by known meaningful releases can be explained by a relativity high detection limit and by a slight role of the sediment as a secondary contamination source. (author). 78 figs., 18 tabs.

  9. Adding a small hydroelectric power plant to the irrigation system of the Liddes community. Feasibility study; Installation d'une petite centrale hydraulique sur le reseau d'irrigation de la commune de Liddes. Etude de faisabilite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This final report prepared for the Swiss Federal Office of Energy (SFOE) examines the possibility of generating electric power with an irrigation system in the southwestern Swiss Alps. The water of the mountain torrent of Palasuit is collected at an altitude of 1620 m above sea-level in a reservoir. It is then passed through a pipeline to the distribution reservoir at 1450 m above sea-level. Among the various schemes studied, the most promising one comprises a Pelton turbine with 2 injectors that utilises a net height-difference of 162 meters. The resulting flow amounts to 150 l/s producing 197 kW of power. In order to achieve this flow, some sections of the pipeline must be replaced by ducts of a larger diameter. If the community of Liddes would obtain the permission to generate power during the whole year, the production would amount to 574,000 kWh/year and the resulting cost 0.090 CHF/kWh.

  10. Plancher solaire direct mixte \\`a double r\\'eseau en habitat bioclimatique - Conception et bilan thermique r\\'eel. Double direct solar floor heating in boclimatic habitation - Design and real energetical balance

    CERN Document Server

    De Larochelambert, Thierry

    2009-01-01

    This study presents a new direct solar floor heating technique with double heating network wich allows simultaneous use of solar and supply energy. Its main purpose is to store and to diffuse the whole available solar energy while regulating supply energy by physical means without using computer controlled technology. This solar system has been tested in real user conditions inside a bioclimatic house to study the interaction of non-inertial and passive walls on the solar productivity. Daily, monthly and annual energy balances were drawn up over three years and completed by real-time measurements of several physical on-site parameters. As a result the expected properties of this technique were improved. The use of per-hour solar productivity, saved primary energy and corrected solar covering ratio is recommended to analyze the performances of this plant and to allow more refined comparisons with other solar systems

  11. Large-scale management of electric grids - Power flux control in an electric network; Gestion des reseaux electriques a grande echelle. Controle des flux de puissance d'un reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    Lalou, M. J. [EIA-FR, Fribourg (Switzerland); Affolter, J.-F. [HEIG-VD, Yverdon-les-Bains (Switzerland)

    2010-07-01

    Security of supply in electricity networks is a major industrial challenge for the future. It involves power generation, transmission and distribution altogether. Networks gradually approach saturation. This article presents an optimal real-time management method of power flux in an electricity network equipped with FACTS and phase measuring devices. FACTS have to be gradually introduced, however not earlier than in 10 years time, as the reliability of data transmission for their control is currently not high enough, considering the order of magnitude of the involved electric power.

  12. INISTE. Directory of the International Network for Information in Science and Technology Education = Repertoire du reseau international d'information concernant l'enseignement des sciences et de la technologie.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Paris (France). Div. of Science, Technical and Environmental Education.

    The objectives of this directory are to provide information which will strengthen regional and international cooperation and research in the field of science and technology education, and to promote greater exchange of ideas and information using new information technologies between institutions. This directory is the first issue of the…

  13. Thematic outlook: the technical survey for the fuel cell research network PACO. August 13, 2004 update no. 26; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 13 aout 2004, no. 26

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage. Their different titles are given below: 1)fuel cells, hydrogen and energy supply in Australia 2)fuel cell system 3)exergy analysis of a SOFC fed with ethanol or methane 4)a SOFC fed with H{sub 2}S 5)challenges to take up in the control of fuel cells systems: role of dynamic models 6)hydrogen as energetic vector for autonomous applications based on renewable energy sources 7)passive feed system for DMFC 8)heterogeneous poly-acids membrane for DMFC 8)ferritic stainless steel assessment as interconnections material for SOFC 9)an approach of super-capacitors integration in fuel cell electric-powered vehicles and emulation of the electric characteristics of the cell using an automatic converter 10)catalytic activity correlations: the difficult case of the hydrogen production from ammonia 11)desulfurization of natural gas and LPG, for reformers, in fuel cells systems 12)Cu/ZnO and Cu/ZnO/Al{sub 2}O{sub 3} catalysts prepared by an homogeneous precipitation method, and used for methanol steam reforming 13)renewable energy production: role of gas and electric power networks to cope with the intermittence problem, with the hydrogen production and storage 14)comparison of the hydrogen adsorption on carbon nano-structures 15)hydrogen detector based on an optical and an electric switching. The references of these articles are detailed. (O.M.)

  14. Energy modelling of PREMIO platform, an architecture dedicated to dynamic load management on electric grid; Modelisation energetique de la plate-forme PREMIO, une architecture dediee a la gestion dynamique de la charge sur le reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    Bougnol, P.; Imbert, P. [ParisTech et l' Institut EIFER, 75 (France); Chartres, S. [Institut EIFER (France); Normand, O. [EDF R and D/EIFER (France)

    2010-01-15

    The cluster of competencies CapEnergies in co-founding with the PACA region launched PREMIO project in 2008. This project aims at implementing a local, innovative and replicable energy architecture that optimizes dynamic load management in order to diminish constraints on power grid and CO{sub 2} emissions. Technical, economical, environmental and sociological impacts of this technology are a strategic issue for project partners that elaborate the business models and future energy policies.The model developed in the frame of PREMIO project provides at local scale (district to city) a method to assess the impact of PREMIO architecture on load curve, CO{sub 2} emissions and energy savings. A case study realised in Lambesc (PACA region) simulates two deployment scenarios of manageable systems: 100% and 50% of the territory consumption, storage and production usages. The exploitation of these results gives prospects to improve this model. (authors)

  15. Analyzing the operation safety of the power grid: towards a more exhaustive approach of the possible futures; Analyser la surete d'exploitation du reseau electrique: vers une approche plus exhaustive des futurs possibles

    Energy Technology Data Exchange (ETDEWEB)

    Paul, J.Ph.; Pompee, J. [Reseau de Transport d' Electricite, Dept. Methodes et Appui, 92 - La Defense (France); Testud, G. [Reseau de Transport d' Electricite, Dept. Exploitation du Systeme Electrique, 92 - La Defense (France)

    2004-09-01

    The general trend in Europe is to push the operation of power system towards their limits. The European electricity market, now fully operational, has increased the variability of operation conditions. As a result, the estimation of security level of the system is a more and more difficult task. This estimation needs to investigate a big number of various situations and sometimes to take into account some transient phenomena. NGT and RTE, the English and the French Transmission System Operators, have commonly developed a method and an associated tool, adapted to this type of analysis. In order to extract the important information, which is hidden in a very big volume of results, they use statistical techniques, such as 'data-mining', initially tuned for... marketing studies. This article is followed by a complementary article written by NGT, which present the ASSESS usages at NGT. (author)

  16. Résultats du REMI (REseau de surveillance MIcrobiologique) du Traict de Pen Bé (Loire-Atlantique) à la pointe du Payré (Vendée) Année 1996

    OpenAIRE

    IFREMER

    1997-01-01

    Le REMI est l'un des 3 réseaux de surveillance du milieu marin gérés par l'IFREMER. Il concerne la surveillance bactériologique des eaux littorales. Historiquement, l'objectif principal de la surveillance bactériologique du milieu marin en France était la salubrité des bivalves pour la consommation humaine. En 1989, sa mission a été étendue à la surveillance de l'environnement. L'organisation actuelle du réseau répond à ces deux objectifs. L'appréciation de la contamination bactériolo...

  17. Thematic outlook. Technical outlook for the fuel-cell research network (PACo). August 8, 2002 update, no. 7; Veille thematique. La veille technique pour le reseau PACo. Actualisation du 8 aout 2002, no. 7

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-08-01

    This report brings together a compilation of abstracts of articles about some recent research works carried out in the domain of fuel cells, hydrogen production and hydrogen storage: characteristics of a PEM fuel cell submitted to a transient increase of CO concentration; perspectives of the different fuel cell technologies in transport applications; development of a portable fuel cell system for the soldier's equipment; direct 2-propanol low temperature fuel cell; a fuel cell system quasi-directly supplied with methanol and based on a mixture of electrolytic polymers; catalysis in low temperature fuel cells (part.1: the cathode stakes); manufacturing and performance of the new multi-layer cathodes for SOFCs; patent about intermediate plates for the limitation of the movement of the bolts used for the fastening of a fuel cell core; the Paul Sherrer institute is testing a zinc and synthetic gas production process that use solar energy, natural gas and zinc oxide; experimental evaluation of a combined plasma/catalyst system for the production of hydrogen from the partial oxidation of hydrocarbons; hydrogen production using a new chemo-organo-trophic bacteria: Citrobacter sp. Y19; hydrogen as energy vector: competition or complementarity with fossil fuels; hydrogen storage in general and in light monocrystalline metal hydrides in particular; the nuclear option for the production of hydrogen as energy of the future. (J.S.)

  18. Thematic outlook: the technical survey for the fuel cell research network PACO. May 18, 2004 update no. 24; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 18 mai 2004, no. 24

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage. Their different titles are given below: 1)comparison of the costs between the electric-powered vehicles and the fuel cell vehicles 2)fuel savings of hydrogen fuel cell vehicles 3)direct formic acid fuel cells producing high current density 4)use of biogas in a SOFC 5)test unit for the determination of the seasonal performance of fuel cell residential systems 6)new management technique of fuel cells for optimizing the output power and the fuel use yield 7)SOFC/micro-turbines systems: current progress achieved and future development 8)advances in the field of the PEMFC 9)advances in the field of new materials for PEMFC 10)hybridization of fuel cell vehicles: an accessible solution for efficient traction systems 11)exergy life cycle analysis of systems of production and storage of hydrogen for mobile application 12)hydrogen and electric power production from coal with CO{sub 2} sequestration 13)H{sub 2}S low temperature removal with ZnO in gas mixtures containing vapor for applications in fuel cells 1-ZnO particles and extruded compounds 14)catalytic nano-composite membranes for CO abatement in fuel cell applications 15)activity and stability of Cu-CeO catalysts in the water-gas conversion for fuel cell applications 16)hydrogen production for fuel cell by ethanol partial oxidation on a nickel based catalyst 17)micro-reactor for hydrogen production in micro-fuel cell applications 18)hydrogen storage capacity and electrochemical properties of the electrode material La{sub 0.7}Mg{sub 0.3}Ni{sub 3.825}Co{sub 0.675}Mn{sub x}(A) 19)hydrogen storage and desorption capacity of carbon nano-tubes. The references of these articles are detailed. (O.M.)

  19. Thematic outlook. Technical outlook for the fuel-cell research network (PACo). January 31, 2002 update, no. 5; Veille thematique. La veille technique pour le reseau PACo. Actualisation du 31 janvier 2002, no. 5

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-01-01

    This report brings together a compilation of abstracts of articles about some recent research works carried out in the domain of fuel cells, hydrogen production and hydrogen storage: fuel cells and fuel cell systems with a non-aqueous electrolyte; the co-development of a fuel cell electrode by Mitsubishi Materials, Kansai Electric Power and Fine Ceramics; a tubular fuel cell under development at the Japan national institute of advanced industrial sciences and technologies (AIST); a patent for an essential component (electro-catalyst) of Medis Technologies' fuel cells; a SOFC with good performances; influence of CO concentration and gas pressure on the performances of a PAFC-type cell; analysis of the life cycle of hydrogen fuel: a methodology for a strategic approach of decision; R and D of a clean hydrogen-based energy system; Teledyne Energy Systems proposes a new hydrogen production system; green alga: still more hydrogen; thermodynamic study and optimization of the hydrogen production by Enterobacter aerogenes; large-scale hydrogen production from hydrocarbons; comparative study of fuels for the production of hydrogen on-board of fuel cell powered vehicles; hydrogen storage in carbon nano-structures; fundamental aspects of the Ti-H system: theoretical and experimental behaviour. (J.S.)

  20. Thematic outlook: the technical survey for the fuel cell research network PACO. March 22, 2004 update no. 22; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 22 mars 2004, no. 22

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage and renewable energies. Their different titles are given below: 1)recent developments in the field of polymer electrolyte fuel cells membranes running above 100 C 2)a new study method of a two-phase flow in a direct methanol fuel cell 3)fuel cell system 4)direct polymer electrolyte fuel cells running with dimethyl ether for portable applications 5)new fuel cells developments for aerospace equipment 6)anode materials for SOFC 7)application of the fuel cell technology to the rail transport 8)hydrogen production by hydrocarbons steam reforming on Ni or Fe based catalysts which are modified by an alkaline earth metal 9)thermochemical hydrogen production from Pacinum virgatum plant 10)development of a catalyst for biomass gasification, in a double-bed gasifier 11)the role of hydrogen in the development of wind power electric systems: the case of Ireland 12)feasibility study of a hydrogen distribution basic equipment for fuel cells vehicles, based on the use of electric power produced in off-peak hours in Japan 13)'bio-hydrogen' production: future developments and limits to a practical application 14)improvement of the hydrogen production from a biomass gasification process, indirectly heated. Removal of carbon dioxide releases with a new biological reformer 15)storage of hydrogen cooled with liquid nitrogen 16)ten years of running of a renewable energy production system based on hydrogen. The references of these articles are detailed. (O.M.)

  1. Thematic outlook. Technical outlook for the fuel-cell research network (PACo). October 23, 2001 update, no. 2; Veille thematique. La veille technique pour le reseau PACo. Actualisation du 23 octobre 2001, no. 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-10-01

    This report brings together a compilation of abstracts of articles about some recent research works carried out in the domain of fuel cells, transportation systems, hydrogen production and energy in general: Japan foresees 10 million of clean vehicles from now to 2010; the fuel cell: a new clean and efficient energy source; development of biogas production units for fuel cells; thermodynamic analysis of an hydrogen-supplied proton conductive SOFC; materials for the high temperature reduction of oxygen in a SOFC; stability of of SOFC components; LaSrMnO metal interface for SOFCs; oxygen ion transport in SOFCs; test of a fuel cell-powered bus in 2002; comparative study of fuels for the on-board hydrogen production; electricity and hydrogen cogeneration from natural gas without emission; solar thermal decarbonization of natural gas; IFC patent: self-thermal reformer of gas fuel for a fuel cell system; some considerations about water electrolysis from a solution of sodium hydroxide; hydrogen production from high temperature water; a more compact and efficient wind turbine. (J.S.)

  2. Deformation and concentration fluctuations under stretching in a polymer network with free chains. The ``butterfly`` effect; Fluctuations de deformation et de concentration sous etirement dans un reseau polymere contenant des chaines libres. L`effet ``papillon``

    Energy Technology Data Exchange (ETDEWEB)

    Ramzi, A.

    1994-06-01

    Small Angle Neutron Scattering gives access to concentration fluctuations of mobile labeled polymer chains embedded in a polymer network. At rest they appear progressively larger than for random mixing, with increasing ratio. Under uniaxial stretching, they decrease towards ideal mixing along the direction perpendicular to stretching, and can grow strongly along the parallel one, including the zero scattering vector q limit. This gives rise to intensity contours with double-winged patterns, in the shape of the figure `8`, or of `butterfly`. Random crosslinking and end-linking of monodisperse chains have both been studied. The strength of the `butterfly` effect increases with the molecular weight of the free chains, the crosslinking ratio, the network heterogeneity, and the elongation ratio. Eventually, the signal collapses on an `asymptotic` function I(q), of increasing correlation length with the elongation ratio. Deformation appears heterogeneous, maximal for soft areas, where the mobile chains localize preferentially. This could be due to spontaneous fluctuations, or linked to frozen fluctuations of the crosslink density. However, disagreement with the corresponding theoretical expressions makes it necessary to account for the spatial correlations of crosslink density, and their progressive unscreening as displayed by the asymptotic behavior. Networks containing pending labeled chains and free labeled stars lead to more precise understanding of the diffusion of free species and the heterogeneity of the deformation. It seems that the latter occurs even without diffusion for heterogeneous enough networks. In extreme cases (of the crosslinking parameters), the spatial correlations display on apparent fractal behavior, of dimensions 2 to 2.5, which is discussed here in terms of random clusters. 200 refs., 95 figs., 21 tabs., 10 appends.

  3. Thematic outlook: the technical outlook for the fuel cell research network (PACO). December 8, 2003 update no. 18; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 8 decembre 2003, no. 18

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    Summaries of different recent articles and patents are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage and with the different other energies. Their different titles are given below: 1)fuel cells or internal combustion engine, will it be really a 'or'? 2)fuel cell systems with a high yield using heat generated by the fuel for producing an addition of electricity 3)the design of DFC (Direct Fuel Cell) 4)electricity production by glucose oxidation in bacterial fuels without intermediary 5)simulation of a photovoltaic/micro-hydraulic/hydrogen system for feeding a house in the Alpes area. Part 2: the integrated system 6)recent advances in the field of materials for fuel cells 7)new materials for hydrogen production in PEMFC 8)modelling of the performances of lithium-ion batteries for fuel cells vehicles 9)hydrogen production at low temperature from oxygenated hydrocarbons 10)an alternative system for stationary hydrogen production: the iron sponge reforming cycle 11)a steam reformer for abating the releases of an internal combustion engine 12)hydrogen separation and purification: hydrogen recycling for SOFC 13)hydrogen physico-chemical storage: are carbon nano-tubes good means of hydrogen storage? 14)energetic scenarios until 2050 15)the use of wind energy on the electric network. The references of these articles and patents are detailed. (O.M.)

  4. Drinking-water hydropower station 'Loye' in Grone, Switzerland - Feasibility study; Petite centrale hydro-electrique de Loye sur le reseau d'eau potable de la commune de Grone. Etude de faisabilite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-11-15

    This final report for the Swiss Federal Office of Energy (SFOE) describes a small hydropower project to be realised in Grone, southern Switzerland. The system planed is described. This provides the necessary pressure reduction in the drinking-water supply system between the springs in the mountains and the reservoir in the valley whilst generating electrical power at the same time. A one-injector Pelton turbine that meets all drinking-water quality requirements is used to generate 48 kW of electrical power using the pressure obtained from the height-difference of around 164 metres. The forecast electricity production amounts to 283,000 kWh/y, of which 88,000 kWh in the wintertime. The expected production cost is 0.219 CHF/kWh. A 550 m long pipeline has to be replaced.

  5. Vaye-Planaz drinking-water hydro-power installation; Petite centrale hydro-electrique de Vaye-Planaz sur le reseau d'eau potable de la commune de Grone. Etude de faisabilite. Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-02-15

    This final report for the Swiss Federal Office of Energy (SFOE) presents the concept for a hydro-power installation that uses an existing drinking-water supply system. The report takes a look at the present situation which features ground-water sources, a transport pipeline and two reservoirs. The concept for the use of the available water pressure due to height-difference to drive a turbine and so generate electricity is described. Variants considered are reviewed. Investment and operating costs are examined as are the electricity production and the cost of the power generated. Finally, investments and other financial aspects are looked at and the further course of action is discussed.

  6. Icogne irrigation-network hydro-power installation; Petite centrale hydro-electrique sur le reseau d'irrigation de la commune d'Icogne. Etude de faisabilite

    Energy Technology Data Exchange (ETDEWEB)

    Cordonier, N.; Rey, G. [Nicolas Cordonier et Gilles Rey SA, Sierre (Switzerland)

    2007-01-15

    This final report for the Swiss Federal Office of Energy (SFOE) presents the feasibility study for a hydro-power installation that uses an existing municipal irrigation scheme in the alpine region of Icogne, Switzerland. The report takes a look at the present situation which features, apart from the irrigation scheme itself, mountain torrents and artificial compensation lakes. The concept for the use of the available water pressure due to height-difference between these two lakes to drive a turbine and so generate electricity is described. Variants considered are reviewed. Investment and operating costs are examined as are the electricity production and the cost of the power generated. Finally, investments and other financial aspects are looked at and the further course of action is discussed.

  7. Transportation network development in a liberalized power system, a coordination problem with production; Le developpement du reseau de transport dans un systeme electrique liberalise, un probleme de coordination avec la production

    Energy Technology Data Exchange (ETDEWEB)

    Rious, V

    2007-10-15

    This thesis analyses how the long term coordination between generation and transmission is organised in a liberalized power system. It relies on a modular analysis framework that allows us to separate the coordination mechanisms into distinct modules. The governance structure of the power network completes this analysis framework. In a rationale of institutional complementarity, this governance structure impacts the way the TSOs effectively implement the power flow management. The proactive behaviour of the TSO for the coordination of generation and transmission can be explained in two ways. Firstly, the network investment can be the only effective process of long term coordination between generation and transmission. Secondly, when forecasting network investment, the TSO can prepare the accommodation of the fast-built generation units and detect the potential failures of locational incentive signals. (author)

  8. Decision support for the definition of wind turbine systems adequacy to site specificities and weak electrical networks; Aide a la decision pour la definition d'un systeme eolien, adequation au site et a un reseau faible

    Energy Technology Data Exchange (ETDEWEB)

    Arbaoui, A

    2006-10-15

    A decision support system for the definition of wind turbine systems is developed by taking into account the wind and site characteristics, the wind turbine components and the electrical network properties close to the site. The approach is based on functional analysis, on the investigation of the functional fluxes and on the definition of a model suitable for supporting decision at the preliminary stages of wind turbine design. The complete set of solutions derived from the model is determined using a Constraint Satisfaction Problem solver. The intrinsic capability of the model to support decision is derived from the investigation of the model parsimony, precision, exactness and specialization. The model takes into account performance criteria resulting from knowledge of manufacturers, distributors and investors. These criteria are used to discriminate design alternatives. Design alternatives correspond to choices of site (wind, electric network) and wind turbine architectures (related to 7 design variables). Performance criteria are the cost of electric kWh, the amount of energy being produced and the discounted total cost of the project. Electric network connection to wind turbines is taken into account through slow variations of the voltage and Flickers phenomenon. First, the maximal rate of penetration of the wind turbine energy production is determined. Next, two design alternatives have been investigated to improve wind turbine system integration in electric distribution networks. These alternatives are a reactive power control system and an inertial energy storage system. Inertial storage systems seem to be more expensive than reactive power control systems for this type of application. The influence of site specificities on decision making process has been established through three different sites (a Mediterranean site and two sites located in northern Europe). Profits relative to the cost of kWh appear to be high for Mediterranean sites. Most of the design variables defining wind turbine architectures have a great influence on this cost. Investigations corroborate the necessity to adapt technological choices to the requirements of electric distribution network. Penetration rates may be greatly improved for the sites characterized by high 'X/R' ratios using variable speed systems. (author)

  9. Thematic outlook: the technical survey for the fuel cell research network PACO. November 15, 2004 update no. 28; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 15 novembre 2004, no. 28

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, hydrogen production and storage. Their different titles are given below: 1)commercialization of fuel cells: technical and economical difficulties to the development of a hydrogen basic equipment 2)public directives to promote the acceptance of fuel cells for automotive industry 3)direct alcohol SOFC: current - voltage characteristics and composition of the fuel gas 4)control by the fuzzy logic method of an autonomous or connected to the electric system SOFC 5)determination of the energetic yield of a DMFC by a fuel circulation system 6)an hybrid system SOFC-Stirling motor of 5 kW 7)control system of PEM type APU feed with gasoline 8)development of a DMFC generator of 2W 8)analysis of the life cycle of the hydrogen production processes 9)hydrogen production for fuel cells by ethanol catalytic steam reforming 10)methanol production from coal: what are the aims of the research? 11)application of a membrane electrochemical reactor to the hydrogen production process by iodine-sulfur thermochemical cycles 12)hydrogen storage in carbonated nano-materials: possibilities and challenges. The references of these articles are detailed. (O.M.)

  10. Variable speed electrical actuators. 3, converter-network and converter-motor-load interactions; Entrainements electriques a vitesse variable. 3, interactions convertisseur-reseau et convertisseur-moteur-charge

    Energy Technology Data Exchange (ETDEWEB)

    Bonal, J.; Seguier, G.

    2000-07-01

    This third part of the study of variable speed electrical actuators analyzes the harmonics generated by the converter, which are the main cause of disturbances related to such actuators. It analyzes also the propagation and effects of these harmonics when injected in the power network, and the effects of the use of a static converter for the power supply of the motor. (J.S.)

  11. Atomistic modelling study of lanthanide incorporation in the crystal lattice of an apatite; Etude par modelisation atomistique de l'incorporation de lanthanides dans le reseau cristallin d'une apatite phosphocalcique

    Energy Technology Data Exchange (ETDEWEB)

    Louis-Achille, V

    1999-07-01

    Studies of natural and synthetic apatites allow to propose such crystals as matrix for nuclear waste storage. The neodymium substituted britholite, Ca{sub 9}Nd(PO{sub 4}){sub 5}(SiO{sub 4})F{sub 2}. is a model for the trivalent actinide storage Neodymium can be substituted in two types of sites. The aim of this thesis is to compare the chemical nature of this two sites in fluoro-apatite Ca{sub 9}(PO{sub 4}){sub 6}F{sub 2} and then in britholite, using ab initio atomistic modeling. Two approaches are used: one considers the infinite crystals and the second considers clusters. The calculations of the electronic structure for both were performed using Kohn and Sham density functional theory in the local approximation. For solids, pseudopotentials were used, and wave functions are expanded in plane waves. For clusters, a frozen core approximation was used, and the wave functions are expanded in a linear combination of Slater type atomic orbitals. The pseudopotential is semi-relativistic for neodymium, and the Hamiltonian is scalar relativistic for the clusters. The validation of the solid approach is performed using two test cases: YPO{sub 4} and ScPO{sub 4}. Two numerical tools were developed to compute electronic deformation density map, and calculate partial density of stases. A full optimisation of the lattice parameters with a relaxation of the atomic coordinates leads to correct structural and thermodynamic properties for the fluoro-apatite, compared to experience. The electronic deformation density maps do not show any significant differences. between the two calcium sites. but Mulliken analysis on the solid and on the clusters point out the more ionic behavior of the calcium in site 2. A neodymium substituted britholite is then studied. Neodymium location only induces local modifications in; the crystalline structure and few changes in the formation enthalpy. The electronic study points out an increase of the covalent character the bonding involving neodymium compared with the one related to calcium, particularly in the Nd-F bond. (author)

  12. Prediction des rendements agricoles du mais et du soya, et du deficit en azote du mais a l'aide d'images aeroportees et d'un reseau de neurones a retropropagation

    Science.gov (United States)

    Serele, Zogbo Charles

    2002-01-01

    This thesis explores the suitability of the multi-layer perceptron (MLP) neural network for prediction of corn and soybean yield and for classification of nitrogen corn stressed vegetation. The results show while using a combination of vegetation and texture indices, and topographic data, we can successfully predict corn yield with MLP. The regression coefficient between observed and predicted yield for corn and soybean were respectively 92% and 43% for the middle season image only. This research also indicates that the information from an image is more important when it is acquired during the period of maximum crop biological activity. Thus, MLP developed on the middle season predicts the corn and soybean yield with respective R2 of 89% and 40%. The degree of relationship between the inputs and the outputs and the quality of the data are very important, because they determine the capabilities of the MLP. When the generalization capabilities of the MLP models were tested on a non-corrected crop yield dataset, the results drop to 45% and 36% for corn and soybean respectively. The MLP demonstrated also its capability to discriminate corn nitrogen status during the growing season. MLP models based on image spectral and textural indices reach a Kappa coefficient of 72% for middle season image and 81% for the combined multidate images. Image texture features also provide useful complementary information for the discrimination of different nitrogen stress levels. The best MLP for predicting corn nitrogen status was the one that integrates image features and topographic parameters. It outperforms the first one with an accuracy of 90% for middle season image and 95% for all two images. Evidence that topographic data are a critical discriminatory information source is both obvious and strong. In consequence, crop physiological status monitoring systems require taking into account the combined effects of soil background and canopy architecture. This thesis have highlighted that MLP has a strong potential for detecting corn nitrogen stressed vegetation and that they would help farmers to better manage crop status during the growing season, when there is still time to respond to problems. The other contributions of this study are the development of crop yield data correction and filtering algorithm and the identification of discriminant analysis as the best method for the MLP inputs selection. (Abstract shortened by UMI.)

  13. Thematic outlook: the technical survey for the fuel cell research network PACO. May 31, 2005 update no. 31; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 31 mai 2005, no. 31

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, hydrogen production and storage. Their different titles are given below: 1)Comparison of the carbon formation domains in a SOFC fed with methane 2)efficiencies of energy storage systems using hydrogen and oxygen 3)electric power and water autonomous generator 4)efficiencies of fuel cells systems running with pure H{sub 2} and O{sub 2} for automobiles applications 5)desulfurization of JP-8 fuel, by selective adsorption on an nickel based adsorbent for micro-SOFC 6)fast response methanol detector running in a wide range of concentrations and temperatures 7)microstructural changes of the unit membrane-electrode during aging tests in strongly wet conditions 8)super-capacitors used as an energy storage system in an hybrid fuel cell system for mobile applications 9)comparison of the steam reforming assisted by hexane and iso-octane microwave plasma 10)continuous production of hydrogen by synthesis gas fermentation (water gas conversion by biological way) 11)titanium coated with carbon nano-tubes as a potential mean to store hydrogen 12)hydrogen storage in magnesium nano-structured composite materials 13)simulation of an hydrogen storage system from hydrides thermally coupled with a fuel cell. The references of these articles are detailed. (O.M.)

  14. Thematic outlook: the technical survey for the fuel cell research network PACO. March 14, 2005 update no. 29; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 14 mars 2005, no. 29

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells and hydrogen production. Their different titles are given below: 1)thermodynamic analysis of a SOFC fed with ethanol and running in internal reforming mode 2)effect of the methanol and ethanol permeation on the efficiencies of a direct alcohol fuel cell with a PtRu/C anode 3)analysis of an hybrid SOFC micro-turbine micro-generation system 4)dynamic modelling and simulation of a small hybrid wind-fuel cell system 5)simulation of a system combining SOFC and PEMFC 6)assessment of the impacts and of the economical aspects of the fuel cell APU part 1: modelling of the cost and the efficiencies of the system part 2)impacts on the health and on the environment, analysis of the life cycle and optimization 7)efficiencies of vehicles equipped with direct hydrogen or reformed methanol PEMFC 8)methods for supplying fuel cell devices 9)auxiliary fuel cell system 10)analysis of life cycle of maritime applications of fuel cells 11)critical analysis of different hydrogen production and uses ways 12)comparison of the hydrogen and natural gas production processes in a thermodynamical and environmental point of view 13)research and development on the hydrogen production by high temperature electrolysis. The references of these articles are detailed. (O.M.)

  15. Thematic outlook: the technical outlook for the fuel cell research network (PACO). December 22, 2003 update no. 19; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 22 decembre 2003, no. 19

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    Summaries of several recent articles and patents are gathered here. They deal with fuel cells, the means of transport, the hydrogen production and with the different new other energies. Their different titles are given below : 1)gas turbine/fuel cell arrangement 2)design and fabrication of a SOFC by CERAMIC FUEL CELLS 3)a 'microbial' fuel cell able of converting glucose in electricity with high yields and velocity 4)a hybrid system: combined cycle gas turbine - multi-stage SOFC 5)a SOFC as auxiliary generator of electricity in an aircraft 6)recent development results of fuel in the Juelich research center 7)state of development of the SOFC at Haldor Topsoe/Risoe 8)a cost/advantage analysis of 'clean cars': methodology and applications to the electric cars 9)the generation of current and heat in a aerostat 10)hydrogen free from CO, produced from bio-ethanol steam reforming on cobalt catalysts supported on ZnO. Effect of the metallic precursor 11)device and method based on the cyclic auto-thermal reforming 12)the ammonia, source of hydrogen for a hybrid system: alkaline fuel/battery 13)effect of the Nafion on the activity of Pt-Ru electrocatalysts for the methanol electro-oxidation 14)'VISION 21': an integration of systems based on coal. The references of these articles and patents are detailed. (O.M.)

  16. Thematic outlook: the technical survey for the fuel cell research network PACO. June 14, 2004 update no. 25; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 14 juin 2004, no. 25

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage. Their different titles are given below: 1)the American plan of de-regulated electric power production with fuel cells 2)application of single wall carbon nano-tubes in fuel cells 3)scenarios of SOFC introduction on the Japanese market 4)advanced SOFC technology and developments at the Siemens Westinghouse firm 5)manufacture and optimal size of a PEMFC cogeneration system for multi-residential application: application to the decomposition strategy 6)analysis of the life cycle of fuel cells using disposal gas 7)technical and economical analysis of a three-generation SOFC system 8)use of APU-FC for an average class tactical lorry, during a use in partial electric power supply or in condition of a silent stand by 9)modelling of the current supply of a lorry at stop: comparison between a idling engine and a APU 10)production of hydrogen and carbon nano-tubes by methane decomposition in a two-stage fluidized bed reactor 11)hydrogen production by auto-thermal reforming of gas-oil 12)simulations of cold starting of a gasoline reformer for mobile applications of fuel cells 13)ethanol production by steam reforming on a Pd/{gamma}Al{sub 2}O{sub 3} trading catalyst 14)control of the CO{sub 2}/(CO+CO{sub 2}) ratio and of the deactivation of the catalyst for the gasoline steam reforming 15)comparison of three integrated processes of partial oxidation producing hydrogen for fuel cells applications 16)search of new methods of the mixture: natural gas/hydrogen production for use in natural gas systems in The Netherlands 17)outline of the energy/hydrogen storage: progress achieved with the different techniques and future prospect of nano-materials 18) hydrogen storage in carbon nano-tubes synthesized by solar way 19)forecast and measurement of the limits of the flammability domain of fuel cells. The references of these articles rae detailed. (O.M.)

  17. Thematic outlook: the technical survey for the fuel cell research network PACO. June 28, 2005 update no. 32; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 28 juin 2005, no. 32

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, hydrogen production and storage. Their different titles are given below: 1)advantages of the use of hydrogen compared with kerosene 2)comparative study of three types of microbial fuel cells 3)carbon nano-tubes for electric applications 4)separation of the CO{sub 2} during the hydrocarbons gasification 5)hydrogen production from hydrocarbons assisted by plasma 6)plasma pyrolysis of biomass for synthesis gas and carbonaceous adsorbent production 7)fast starting reformer for automobile applications 8)development of a unit of hydrogen production from LPG for PEM systems: assessment at the laboratory scale of the sub-units of auto-thermal reforming and of selective oxidation 9)hydrogen and electric power production, with CO{sub 2} sequestration, by redox species chemical cycle reforming (CuO, Mn{sub 2}O{sub 3}, NiO, Fe{sub 2}O{sub 3} and SiO{sub 2} as support) 10)exergy and economic analysis of a PEM electrolyser running at different temperatures and pressures 11)nano-structured materials for hydrogen storage 12)composite materials based on light elements for hydrogen storage. The references of these articles are detailed. (O.M.)

  18. Thematic outlook: the technical outlook for the fuel cell research network (PACO). September 23, 2003 update no. 16; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 23 septembre 2003, no. 16

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    Summaries of several recent articles and patents are gathered here. They deal with fuel cells and hydrogen production and storage. Their different titles are given below: 1)the behaviour of the electrode potential in direct hydrazine fuels 2)a device of desalination fed with a fuel cell 3)experiment in the field of residential fuel cell systems at ECN (Energy Research Center) 4)'design of a divided feeding' for SOFC with an internal reforming system 5)water management and thermal management in a fuel cell vehicle fed with hydrogen extracted from sodium borohydride (NaBH{sub 4}) 6)a mathematical model of propulsion systems by PEMFC for mobile applications 7)assessment of the feasibility of a DMFC containing an alkaline membrane 8)semi-empirical assessment model of the performance of a DMFC, first part: development of the model and validation 9)PEMFC and the challenge of CO 10)materials for SOFC 11)natural gas and LPG desulfurization for fuel cells reformers 12)heat exchangers for reforming techniques 13)desulfurization of a fuel for fuel cell system 14)hydrogen production from solar thermal reactor 15)hydrogen physico-chemical storage: nano-structured storage materials having modified covalent bonds sp2. The references of these articles and patents are detailed. (O.M.)

  19. Analysis and modeling of low voltage electrical network at power line carrier frequencies (3-148.5 kHz); Analyse et modelisation du reseau basse tension aux frequences courants porteurs (3 KHZ-148,5 KHZ)

    Energy Technology Data Exchange (ETDEWEB)

    Duval, G.

    1998-07-01

    Electricite de France (EdF) wishes to establish a physical communication link between his clients and the EdF centres. The final link, i.e. between the high/low voltage transformation substation and the residential clients, being ensured by carrier currents. With this aim, an analysis and a modeling of the low voltage network at the carrier frequencies (3 kHz - 148.5 kHz) has been performed. This work has been carried out in parallel with an experiment involving 3500 apparatuses that use carrier currents. The diversity of the French low voltage networks and the limitations imposed by the EN50065-1 standard about the use of carrier currents in Europe do not favour the development of such carrier current systems. Disturbing voltages and localized impedances represent the main difficulties to get round. Inside accommodations, domotic carrier currents have a reduced range but a higher disturbance amplitude because of the proximity of appliances. A differential mode to common mode conversion phenomenon has been evidenced which generates network couplings and important electromagnetic fields. Energy lines and cables have been analyzed using numerical models. Load peaks have been analyzed using statistical tools in order to take into account the daily fluctuations. The modeling of the network is made in two steps: a double-wire model is considered first. Then a three-phase model is developed which analyzes the inter-phases coupling and the effect of the distribution of clients' loads on each phase. The results of this model are conformable with measurements except for underground networks. As perspectives of future works and beyond todays standard framework, the techniques that allow a sensible increase of communication flow rates have been reviewed. (J.S.)

  20. Thematic outlook: the technical outlook for the fuel cell research network (PACO). July 21, 2003 update no. 14; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 21 juillet 2003, no. 14

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    Summaries of several recent articles and patents are gathered here. They deal with fuel cells and hydrogen production and storage. Their different titles are given below: 1)fuel cells fed with wood in different buildings 2)use of CO{sub 2} produced in a SOFC in a reactor in which algae are produced 3)PEMFC systems: the requirement of using polymers running at higher temperatures to better manage the heat and water 4)a technical and economical assessment of a fuel cell system decoupled of the electric network to feed buildings located in Sweden 5)use of ammonia as fuel in SOFC 6)direct oxidation fuel cells running with pure propanol-2 6)fuel cells at protonic ceramics for a running at a higher yield with methane 7)future role of gold in fuel cell systems 8)a heat management system in a fuel cell 9)tests in real conditions of the running of a fuel cell hybrid bus 10)a hydrogen production unit from hydrocarbons without releases for fuel cells used for mobile and portable applications 11)development of a methanol reformer for fuel cells 12)the reforming of liquid hydrocarbons for fuel cells used in transport applications 13)the properties of hydrogen storage in metallic hydrides with vanadium having a centred cubic structure. The references of these articles and patents are detailed. (O.M.)

  1. Thematic outlook. Technical outlook for the fuel-cell research network (PACo). June 15, 2001 update, no. 0; Veille thematique. La veille technique pour le reseau PACo. Actualisation du 15 juin 2001, no. 0

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-06-01

    This report brings together a compilation of abstracts of articles about some recent research works carried out in the domain of fuel cells, hydrogen production and hydrogen storage: effect of hydrogen on the magnetic properties of iron nano-crystalline particulates; smaller fuel cells; development of a 1 kW PEMFC; materials for low temperature SOFCs; development and operation of a 150 W DMFC; materials science and engineering: a key-technology for the commercialization of fuel cells; chemical and electrochemical behaviour of Ni-Ti in cathodic conditions as used in a MCFC; patent for stainless steel bipolar plates; development of proton conductive membranes for PEMFCs and DMFCs; development and characterization of acid-doped polymer mixtures (poly-benzimidazole/sulfonated poly-sulfone) used as fuel cell electrolyte; modification of a proton conductive membrane for the reduction of methanol diffusion in a DMFC; DMFC based on a new low cost nano-porous membrane; method for controlling the connexion between a fuel cell and a power grid; steam reforming of biomass-derived ethanol for hydrogen production devoted to fuel cells; hydrogen production by catalytic decomposition of methane; hydrogen storage. (J.S.)

  2. Thematic outlook. Technical outlook for the fuel-cell research network (PACo). December 7, 2001 update, no. 3; Veille thematique. La veille technique pour le reseau PACo. Actualisation du 7 decembre 2001, no. 3

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-12-01

    This report brings together a compilation of abstracts of articles about some recent research works carried out in the domain of fuel cells, hydrogen production and hydrogen storage, and of renewable energies in general: studies of the humidification of PEMFCs; chemical engineering in the automotive industry; environmental impact of the manufacturing of tubular and planar SOFCs; fullerenes for portable fuel cells; hydrogen fuel produced from the gasification of solid municipal fuels; required electric power for a large-scale production of hydrogen for vehicles; hydrogen produced from methane decomposition: an application for the decarbonization of fossil fuels; production of synthesis gas from ethanol vapo-reforming; electrochemical characterization of the hydrogen adsorption capacity of carbon nano-tubes prepared by chemical vapor deposition (CVD), electric arc and laser ablation techniques; hydrogen storage in ultrasound wave processed carbonized materials; hydrogen storage in fuel cell-powered vehicles; year 2000 status of renewable energies in France; renewable energy sources: too insufficient efforts made in the UK; wind turbine in the country of wind mills (Netherlands); a research institute for wind energy in the Netherlands; feasibility study of underwater CO{sub 2} storage. (J.S.)

  3. Neural network and noise injection for the modeling of ozone pollution; Reseau de neurones et injection de bruit pour la modelisation de la pollution par l'ozone

    Energy Technology Data Exchange (ETDEWEB)

    Pigeon, E.; Massieu, J.F. [Laboratoire d' Automatique de Procede, 14 - Caen (France)

    2001-07-01

    This study has been carried out in the framework of a collaboration between the laboratory of processes automation (LAP, Caen (France)), and Air Com, a monitoring network for the prevention of atmospheric pollution in Basse-Normandie. It aims at obtaining a medium and long term forecast of the ozone level above the Caen city. The expected goal is to foresee the pollution peaks exceeding the warning thresholds, but the rareness of such events make them more difficult to predict. In order to solve this kind of problem, a neural modeling method combined with a noise injection technique has been implemented in order to obtain a sufficiently performing model over the whole domain of operation. (J.S.)

  4. Thematic outlook: the technical outlook for the fuel cell research network (PACO). December 8, 2003 update no. 18; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 8 decembre 2003, no. 18

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    Summaries of different recent articles and patents are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage and with the different other energies. Their different titles are given below: 1)fuel cells or internal combustion engine, will it be really a 'or'? 2)fuel cell systems with a high yield using heat generated by the fuel for producing an addition of electricity 3)the design of DFC (Direct Fuel Cell) 4)electricity production by glucose oxidation in bacterial fuels without intermediary 5)simulation of a photovoltaic/micro-hydraulic/hydrogen system for feeding a house in the Alpes area. Part 2: the integrated system 6)recent advances in the field of materials for fuel cells 7)new materials for hydrogen production in PEMFC 8)modelling of the performances of lithium-ion batteries for fuel cells vehicles 9)hydrogen production at low temperature from oxygenated hydrocarbons 10)an alternative system for stationary hydrogen production: the iron sponge reforming cycle 11)a steam reformer for abating the releases of an internal combustion engine 12)hydrogen separation and purification: hydrogen recycling for SOFC 13)hydrogen physico-chemical storage: are carbon nano-tubes good means of hydrogen storage? 14)energetic scenarios until 2050 15)the use of wind energy on the electric network. The references of these articles and patents are detailed. (O.M.)

  5. The remote management optimizes the geothermal district heating network of Chevilly-Larue and l'Hay-les-Roses; La telegestion optimise le reseau de chaleur geothermique de Chevilly-Larue et l'Hay-les-Roses

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2003-11-01

    The installation of a new remote control system for the management of the district heating network of Chevilly-Larue and l'Hay-les-Roses cities (Paris region, France) has permitted to optimize the use of the different energy sources (geothermal, cogeneration, fuel-oil and natural gas) supplying the network and to better adjust the control of the production means. (J.S.)

  6. Implementation of FCI heating system to the control system of Tore-Supra; Integration du systeme de chauffage FCI au sein du reseau de controle commande du Tokamak Tore Supra

    Energy Technology Data Exchange (ETDEWEB)

    Wisniewski, S

    2001-11-01

    This report presents the implementation of the ion cyclotron resonance heating system (FCI) to the instrumentation and control system of the Tore-Supra tokamak. The new plasma heating system involves 3 antennas delivering 12 MW that are required to maintain fusion reactions. This paper is divided into 8 chapters: 1) thermonuclear fusion and Tore-Supra tokamak; 2) hardware system around Tore-Supra, in this chapter the control system and the data acquisition and processing systems are presented; 3) functional analysis, this analysis defines the different needs concerning timing and pilot-controlling, a preliminary proposition of hardware equipment is made; 4) operating modes of FCI; 5) communication within the control system network; 6) communication with the supervisory system of the power stations; 7) management of data exchange with SMX generators; and 8) control of the rate of stationary waves during the injection of power into the plasma.

  7. Thematic outlook: the technical survey for the fuel cell research network PACO. April 16, 2004 update no. 23; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 16 avril 2004, no. 23

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage. Their different titles are given below: 1)the importance of the fuel choice in the efficiency of the SOFC 2)assessment model of a centralized electric power system: SOFC/gas turbine 3)a direct borohydride fuel cell 4)thermodynamic optimization of the internal structure of a fuel cell 5)a SOFC/gas turbine cogeneration system 6)micro-fuel cells for portable applications 7)comparison of performances of direct alcohol fuel cells using different anode catalysts 8)materials and designs for SOFC used in stationary and mobile applications 9)development of measurement techniques for two-phase hydrogen weight flow 10)semi-conductors potentiometric probe for the hydrogen detection in air 11)integration of a fuel cell in an electric system, using a regulator based on the theory of regulation by disturbances accommodation 12)'greening London's black cabs': a study of the use potential of fuel cells cabs in London 13)gasification of leather residues - part 1 - experimental study in a pilot gasifier with a descending current 14)use of composite conducting membranes to produce hydrogen by water dissociation 15)molybdenum carbide based catalyst for the water gas reaction used in fuel cells vehicles applications 16)hydrogen production process from methane hydrates with carbon dioxide hydrates sequestration 17)comparative study between the hydrogen adsorption on super activated carbon and on carbon nano-tubes. The references of these articles are detailed. (O.M.)

  8. Thematic survey: the technical survey for the PACo network. Actualization of the 30 september 2003, no.8; Veille thematique: la veille technique pour le reseau PACo. Actualisation du 30 septembre 2002, no.8

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    This document provides the thematic survey concerning the hydrogen fuel cell, realized on september 2003. The papers published during this period deal with the hydrogen fuel cell (low power stationary, mobile and handling applications, components, vehicles), the hydrogen production (the ethanol reforming), hydrogen storage (materials activation), safety, regulation and standards, carbon dioxide emissions decrease and fuel production. For each purposes abstracts are provided. (A.L.B.)

  9. Definition of a reference metrology network for the positioning of a large linear accelerator; Definition d'un reseau de reference metrologique pour le positionnement d'un grand accelerateur lineaire

    Energy Technology Data Exchange (ETDEWEB)

    Becker, F

    2003-12-15

    This thesis is a study of the Compact Linear Collider (CLIC) alignment system, a project of linear accelerator of about 30 km long of the European Organization for Nuclear Research (CERN). The pre-alignment tolerance on the transverse positions of the components of the CLIC linacs is typically ten microns over distances of 200 m. This research is a consequence of 10 years work, where several sets of special sensors dedicated to metrology have been adapted for the CLIC project. Most of these sensors deliver measurements linked to geometric references sensitive to gravity fluctuation. An important part of this work is therefore dedicated to study the gravity disruptions as a high level of accuracy is required. The parameters to take into account in the use of the hydrostatic leveling have thus been highlighted. A proposal of configuration of the system alignment based on a selection of sensors has also been given in this research. Computer models of different possible configurations have been presented. As the existing computing software was inappropriate, a new object oriented software package has been developed, to ensure future upgrades. An optimized configuration of the network has been defined from a set of simulations. Finally, due to problems in the use of hydrostatic leveling systems, a solution based on the use of a long laser beam as an alternative solution is discussed. (author)

  10. Thematic outlook: the technical survey for the fuel cell research network (PACO). January 28, 2004 update no. 20; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 15 janvier 2004, no. 20

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage. Their different titles are given below: 1)the characteristics of PEMFC running at negative exterior temperatures 2)the effects of CO poisoning on PEMFC at temperatures until 200 C 3)the mechanical properties of tubular SOFC 4)fuel cells fed with coal 5)analysis of cogeneration system: planar SOFC/gas turbine 6)modelling of fuel cells for transport, according to the neuronal networks method 7)design of hybrid fuel cell systems 8)a comparative study of direct methanol fuel cells for vehicles 9)desulfurization by adsorption and catalytic steam reforming of gas oil for applications in fuel cells 10)feasibility study of hydrogen production for fuel cell vehicles by naphtha on-board steam reforming 11)reforming catalyst of kerosene for fuel cell, kinetics and modelling of steam reforming 12)reforming by partial oxidation of low lubricant power gas oil, of dimethyl ether and methane for SOFC 13)conversion of solar heat in fuels by solar thermochemistry 14)hydrogen purification for fuel cells: selective oxidation of CO on Pt-Fe / zeolite catalysts 15)hydrogen photo-production from cellulose derived compound, with a system: chlorophyll / platinum nano-particles 16)hydrogen storage in commercial activated carbon. The references of these articles are detailed. (O.M.)

  11. Selection and integration of a network of parallel processors in the real time acquisition system of the 4{pi} DIAMANT multidetector: modeling, realization and evaluation of the software installed on this network; Choix et integration d`un reseau de processeurs paralleles dans le systeme d`acquisition temps reel du multidetecteur 4{pi} DIAMANT: modelisation, realisation et evaluation du logiciel implante sur ce reseau

    Energy Technology Data Exchange (ETDEWEB)

    Guirande, F. [Ecole Doctorale des Sciences Physiques et de l`Ingenieur, Bordeaux-1 Univ., 33 (France)

    1997-07-11

    The increase in sensitivity of 4{pi} arrays such as EUROBALL or DIAMANT has led to an increase in the data flow rate into the data acquisition system. If at the electronic level, the data flow has been distributed onto several data acquisition buses, it is necessary in the data processing system to increase the processing power. This work regards the modelling and implementation of the software allocated onto an architecture of parallel processors. Object analysis and formal methods were used, benchmark and evolution in the future of this architecture are presented. The thesis consists of two parts. Part A, devoted to `Nuclear Spectroscopy with 4 {pi} multidetectors`, contains a first chapter entitled `The Physics of 4{pi} multidetectors` and a second chapter entitled `Integral architecture of 4{pi} multidetectors`. Part B, devoted to `Parallel acquisition system of DIAMANT` contains three chapters entitled `Material architecture`, `Software architecture` and `Validation and Performances`. Four appendices and a term glossary close this work. (author) 58 refs.

  12. The role of dopamine D-3, 5-HT2(A) and 5-HT2(C) receptor variants as pharmacogenetic determinants in tardive dyskinesia in African-Caribbean patients under chronic antipsychotic treatment Curacao extrapyramidal syndromes study IX

    NARCIS (Netherlands)

    Wilffert, B.; Al Hadithy, A. F. Y.; Sing, V. J.; Matroos, G.; Hoek, H. W.; van Os, J.; Bruggeman, R.; Brouwers, J. R. B. J.; van Harten, P. N.

    2009-01-01

    Tardive dyskinesia (TD) is associated with polymorphisms of the dopamine D-3, serotonin 2A and 2C receptors (DRD3, HTR2A and HTR2C, respectively). This study investigated the possible relationship between TD and the polymorphisms Ser9Gly (DRD3), 102T>C (HTR2A), -1438G>A(HTR2A) and Cys23Ser (HTR2C) i

  13. Identification of a cys-ser substitution in the 5-HT{sub 2C} (HTR2C) receptor gene and allelic association to violent behavior and alcoholism

    Energy Technology Data Exchange (ETDEWEB)

    Lappalainen, J.; Ozaki, N.; Goldman, D. [National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (United States)] [and others

    1994-09-01

    Several lines of evidence suggest that brain serotonergic functions, including behavioral and neurochemical responses to 5-HT{sub 2C} agonist, are abnormal in some individuals with alcoholism and aggressive behaviors. The aim of the present study was to identify coding sequence variants in the human 5-HT{sub 2C} receptor gene which may cause abnormal or variant function of this receptor. Using SSCP analysis, a non-conservative cys-ser substitution was found in the 5-HT{sub 2C} receptor (designated 5-HT{sub 2Ccys} and 5-HT{sub 2Cser}). The polymorphism was typed in CEPH families to genetically map the gene. To test for association of the variant to alcoholism, violent behavior and serotonin function, the 5-HT{sub 2C} genotypes of 151 non-related Finnish male alcoholic violent offenders and impulsive fire setters and 127 Finnish psychiatrically interviewed healthy male volunteers were determined. CSF 5-HIAA concentrations were available for 74 alcoholic violent offenders and 25 healthy volunteers. Linkage analysis placed the 5-HT{sub 2C} gene on Xq21, a region that has been previously shown to contain genes for several mental retardation syndromes. The 5-HT{sub 2Ccys}/5-HT{sub 2Cser} genotype frequencies in alcoholic violent offenders and controls differed significantly (0.90/0.10 and 0.82/0.18, respectively, P=0.048). The association was found to be strongest in the violent offenders who did not fulfill the criteria for antisocial personality disorder (5-HT{sub 2Ccys}/5-HT{sub 2Cser} 0.93/0.07, p=0.021). No association was found between CSF 5-HIAA concentrations and 5-HT{sub 2C} genotype. These results implicate a 5-HT{sub 2C} receptor amino acid substitution in predisposition to alcohol abuse and violent behavior in a subgroup of alcoholics.

  14. PRELIMINARY RESULTS OF THE AGC-4 IRRADIATION IN THE ADVANCED TEST REACTOR AND DESIGN OF AGC-5 (HTR16-18469)

    Energy Technology Data Exchange (ETDEWEB)

    Davenport, Michael; Petti, D. A.

    2016-11-01

    The United States Department of Energy’s Advanced Reactor Technologies (ART) Program will irradiate up to six nuclear graphite creep experiments in the Advanced Test Reactor (ATR) located at the Idaho National Laboratory (INL). The graphite experiments are being irradiated over an approximate eight year period to support development of a graphite irradiation performance data base on the new nuclear grade graphites now available for use in high temperature gas reactors. The goals of the irradiation experiments are to obtain irradiation performance data, including irradiation creep, at different temperatures and loading conditions to support design of the Very High Temperature Gas Reactor (VHTR), as well as other future gas reactors. The experiments each consist of a single capsule that contain six stacks of graphite specimens, with half of the graphite specimens in each stack under a compressive load, while the other half of the specimens are not be subjected to a compressive load during irradiation. The six stacks have differing compressive loads applied to the top half of diametrically opposite pairs of specimen stacks. A seventh specimen stack in the center of the capsule does not have a compressive load. The specimens are being irradiated in an inert sweep gas atmosphere with on-line temperature and compressive load monitoring and control. There are also samples taken of the sweep gas effluent to measure any oxidation or off-gassing of the specimens that may occur during initial start-up of the experiment. The first experiment, AGC-1, started its irradiation in September 2009, and the irradiation was completed in January 2011. The second experiment, AGC-2, started its irradiation in April 2011 and completed its irradiation in May 2012. The third experiment, AGC-3, started its irradiation in late November 2012 and completed in the April of 2014. AGC-4 is currently being irradiated in the ATR. This paper will briefly discuss the preliminary irradiation results of the AGC-4 experiment, as well as the design of AGC-5.

  15. Color-specific conditioning effects due to both orange and blue stimuli are observed in a Halobacterium salinarum strain devoid of putative methylatable sites on HtrI.

    Science.gov (United States)

    Lucia, S; Cercignani, G; Frediani, A; Petracchi, D

    2003-01-01

    Behavioral responses of Halobacterium salinarum appear as changes in the frequency of motion reversals. Turning on orange light decreases the reversal frequency, whereas blue light induces reversals. Light pulses normally induce the same response as step-up stimuli. However, anomalous behavioral reactions, including inverse responses, are seen when stimuli are applied in sequence. The occurrence of a prior stimulus is conditioning for successive stimulation on a time scale of the same order of adaptational processes. These prolonged conditioning effects are color-specific. The only adaptation process identified so far is methylation of the transducers, and this could be somehow color-specific. Therefore we tested for the behavioral anomalies in a mutant in which all methylation sites on the transducer have been eliminated. The results show that behavioral anomalies are unaffected by the absence of methylation processes on the transducer.

  16. Olanzapine-induced weight gain is associated with the -759C/T and -697G/C polymorphisms of the HTR2C gene.

    Science.gov (United States)

    Godlewska, B R; Olajossy-Hilkesberger, L; Ciwoniuk, M; Olajossy, M; Marmurowska-Michałowska, H; Limon, J; Landowski, J

    2009-08-01

    Weight gain, a serious problem associated with some antipsychotic drugs, notably olanzapine and clozapine, was suggested to be associated with -759C/T polymorphism of the 5-HT2C receptor gene. This study aimed to examine a potential association of two functional polymorphisms of the promoter region of this gene: -759C/T (rs3813929) and -697G/C (rs518147), with weight gain after 6 weeks of olanzapine monotherapy. It included 107 patients with schizophrenia; among them 36 are first-episode drug-naive patients. Analysis was carried out by PCR-restriction fragment length polymorphism. A protective effect of -759T and -697C alleles was found: significantly less patients with -697C (3/51) and no patient with -759T (0/28) alleles experienced body mass index increase >or=10% (P=0.0006 and 0.002, respectively). The same was true for drug-naive patients possessing any of the variant alleles. There was a significant association of haplotypes with a >or=10% body mass index increase (P=0.001). On the basis of the additional statistical analysis, the more important role of -697C allele was suggested.

  17. Mapping of the serotonin 5-HT{sub 1D{alpha}} autoreceptor gene (HTR1D) on chromosome 1 using a silent polymorphism in the coding region

    Energy Technology Data Exchange (ETDEWEB)

    Ozaki, N.; Lappalainen, J.; Linnoila, M. [National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (United States)] [and others

    1995-04-24

    Serotonin (5-HT){sub ID} receptors are 5-HT release-regulating autoreceptors in the human brain. Abnormalities in brain 5-HT function have been hypothesized in the pathophysiology of various psychiatric disorders, including obsessive-compulsive disorder, autism, mood disorders, eating disorders, impulsive violent behavior, and alcoholism. Thus, mutations occurring in 5-HT autoreceptors may cause or increase the vulnerability to any of these conditions. 5-HT{sub 1D{alpha}} and 5-HT{sub 1D{Beta}} subtypes have been previously localized to chromosomes 1p36.3-p34.3 and 6q13, respectively, using rodent-human hybrids and in situ localization. In this communication, we report the detection of a 5-HT{sub 1D{alpha}} receptor gene polymorphism by single strand conformation polymorphism (SSCP) analysis of the coding sequence. The polymorphism was used for fine scale linkage mapping of 5-HT{sub 1D{alpha}} on chromosome 1. This polymorphism should also be useful for linkage studies in populations and in families. Our analysis also demonstrates that functionally significant coding sequence variants of the 5-HT{sub 1D{alpha}} are probably not abundant either among alcoholics or in the general population. 14 refs., 1 fig., 1 tab.

  18. Reconstrucción craneofacial compleja: malla de titanio, hueso autólogo preservado en óxido de etileno y reconstrucciones tridimensionales en polimetilmetacrilato (HTR-PMI Complex craneofacial reconstruction: titanium mesh, autologous bone preserved in ethylene oxide and tridimensional polimetilmetacrilate implants (HTR-PMI

    Directory of Open Access Journals (Sweden)

    G. Flores-Lima

    2010-03-01

    Full Text Available La evolución de la Cirugía Craneofacial se inicio con Jean Paul Tessier, quien en 1967 preconizó el uso de injertos autólogos de hueso fresco en gran cantidad para cubrir extensas brechas óseas en la corrección de disóstosis craneofacial. Recientemente, diferentes tipos de reconstrucción utilizando hueso autólogo preservado en oxido de etileno y materiales como el polimetilmetacrilato poroso confeccionado a medida, han permitido también la corrección de grandes defectos óseos craneofaciales. Presentamos nuestra experiencia inicial en el uso de estas técnicas a través de un análisis retrospectivo sobre 21 pacientes operados por un equipo multidisciplinario entre Enero del 2007 y Marzo del 2009 en el Hospital Militar, Centro Panamericano de Ojos y Hospital de Diagnóstico de El Salvador, en los que se utilizaron formas alternativas pera reconstrucción de calota craneana, piso de órbita, fosa craneal anterior, área órbito-cigomática y maxilar superior. No registramos casos de infección o retirada de material de osteosíntesis aloplástico o de los injertos autólogos, ni hubo fístulas. Si se presentó una úlcera postraumática en una zona de unión de tejido desvitalizado, que se resolvió con tratamiento conservador. Los resultados estéticos obtenidos fueron de aceptables a buenos. Como conclusión, el equipo multidisciplinario, la combinación de técnicas quirúrgicas y el uso de material protésico para la reconstrucción craneofacial compleja ha dado como resultado avances significativos desde el punto de vista funcional y estético ante lesiones que involucran esta compleja área anatómica.The evolution of craniofacial surgery began with Jean Paul Tessier, who in 1967 supported the use of fresh autologous bone to cover bone defects in the craniofacial area. Recently different types of reconstructions using autologous bone preserved in ethylene oxide and advanced custom-made polimetacrilate implants that have allowed more complex and esthetically rewarding procedures to patients have suffered extensive bone loss in the craniofacial area. We report our initial experience using these techniques with a retrospective analysis of 21 patients operated from January 2007 to March 2009 by a multidisciplinary team in the Hospital Militar, Centro Panamericano de Ojos and Hospital de Diagnóstico of El Salvador, who required complex craneofacial reconstruction using one or more techniques to cover the defects. The reconstructed areas have been cranium, orbital roof and floor as well as cigomatic region, anterior cranium fossa and superior maxillary sinuous. No infections or retrieval of material for reconstruction or fistulas were reported and only one post-traumatic ulcer that resolved in a conservative way. The esthetic results in the patients operated have been considered acceptable, to excellent. As a conclusions, multidisciplinary team, combination of surgical techniques and the acquisition of advanced prosthesis material for craniofacial reconstructions has resulted in a significant advance from the functional and aesthetic point of view in areas that involve this anatomically complex area.

  19. Cold molecules: formation, trapping and spectroscopy. - Piling up of cesium dimers in a quadrupolar magnetic trap. - Spectroscopy by lack of photo-association; Molecules froides: formation, piegeage et spectroscopie. - Accumulation de dimeres de cesium dans un piege quadrupolaire magnetique. - Spectroscopie par frustration de photoassociation

    Energy Technology Data Exchange (ETDEWEB)

    Vanhaecke, N

    2003-10-15

    This thesis deals with the study of cold molecules obtained through the cold atom photo-association technique. Our study is focused both on manipulating external degrees of freedom of these Cs{sub 2} molecules and on studying their internal interactions. A quadrupolar magnetic trap of about 2.10{sup 5} cold Cs{sub 2} molecules is demonstrated. The trap lifetime is on the order of 600 ms, mainly limited by the background gas pressure. The trapped molecules are identified, which allows the determination of the trapped molecule cloud temperature, which is about 35 {mu}K. A dipolar trap is set up by the use of a focused CO{sub 2} laser beam. Moreover, this thesis describes a two-photon photo-association spectroscopy. We report the first study of two-photon photo-association line shapes, which exhibit typical Fano profiles. Taking advantage of the detailed understanding of these line shapes, we measure over one hundred high-lying level energies with an accuracy on the order of 10 MHz. Then we interpret theoretically these energies. The huge hyperfine structure of the Cesium atom binds us to solve a coupled channel Schroedinger equation for internuclear distances above 15 a{sub 0}. Hence we use an asymptotic theory to fit the potential parameters of the Cs{sub 2} dimer. We adjust those parameters by the mean of both evolutionary strategies and deterministic optimum seeking. This is followed by a careful statistics study, which leads to a very accurate determination of the Vander Waals coefficient. Moreover we report the first experimental determination of the exchange interaction amplitude. (author)

  20. Retention of PWR primary coolant trace elements by cation exchange resins during cold shutdown with oxygenation: modelling and experimental results for silver behavior; Retention des elements traces du fluide primaire des REP par les resines echangeuses de cations lors des mises en arret a froid avec oxygenation: modelisation et resultats experimentaux relatifs au comportement de l'argent

    Energy Technology Data Exchange (ETDEWEB)

    Elain, L.; Doury-Berthod, M. [CEA Saclay, INSTN, Institut National des Sciences et Techniques Nucleaires, 91 - Gif-sur-Yvette (France); Genin, J.B. [CEA Cadarache, Dir. de l' Energie Nucleaire (DEN), 13 - Saint-Paul-lez-Durance (France); Berger, M. [Electricite de France (EDF/SEPTEN), 69 - Villeurbanne (France)

    2004-07-01

    In order to minimize the radiochemical impact of the corrosion products on the operation of Pressurized Water Reactors, on-line purification of the primary coolant is carried out. The purification system arranged on the Chemical and Volume Control System is made up of mechanical filters and demineralizers packed with a mixed bed of cation and anion exchange resins. This paper proposes an update on the retention of primary coolant trace elements by the cation exchange resins of the demineralizers during cold shutdowns with oxygenation. The study is first of all devoted to the description of the concentration profiles of the various cation constituents which settle in the demineralizer during purification after oxygenation. For a number of trace elements, localized enrichment zones at the Li{sup +}/Ni(Il) exchange zone are expected to appear in the column. The case of silver is afterwards discussed in detail. Thermodynamic modelling shows that the theoretical retention volume of the metallic element and its degree of enrichment in the column are dependent on the basic composition of the primary coolant and the specific characteristics of the demineralizer cation exchanger. At the Ag{sup +} ion concentration expected in the reactor coolant after oxygenation (between 10{sup -8} mol.L{sup -1} and 10{sup -6} mol.L{sup -1}), the breakthrough of silver should be near-simultaneous with that of nickel. The experimental results, obtained in the laboratory and with a 'Mini-CVCS' pilot instrumentation recently used during the cold shutdown of Tricastin Unit 2,900 MWe PWR NPP, confirm the validity of these theoretical forecasts and enable new hypotheses to be advanced for explaining silver release from a demineralizer. (authors)

  1. Development of asymptotic methods for the study of interactions between cold atoms: determination of scattering lengths of sodium and cesium; Developpement de methodes asymptotiques pour l'etude des interactions entre atomes froids: determination de longueurs de diffusion du sodium et du cesium

    Energy Technology Data Exchange (ETDEWEB)

    T' Jampens, B

    2002-12-15

    Precise knowledge of cold-atom collision properties is essential for the studies of Bose-Einstein condensation or cold molecule formation. In such experiments, the interaction mainly occurs at rather large interatomic distance, in the so-called asymptotic region. We have developed a purely asymptotic method which allows us to fully describe the collision properties of cold alkali atoms without using the inner part of the molecular potentials, which is often known with a poor precision. The key point of the method is the setting of nodal lines, which are the lines connecting the nodes of successive radial wavefunctions near the ground state threshold. Within the framework of Born-Oppenheimer approximation, computing such nodal lines, by numerical integration of the radial Schroedinger equation in the asymptotic region only, provides a very simple way to derive scattering lengths from observed bound level positions. The method has been extended to the multichannel case and appears now as a genuine parametric method, in which a few parameters (some chosen nodal lines) replace the inner part of the potentials. These nodal lines are used as fitting parameters, which are adjusted on experimental results. Once these parameters have been determined, any collision property such as scattering lengths, clock shifts or magnetic field induced Feshbach resonances can be deduced in principle. This method has been applied to obtain the collision properties of ultracold sodium and cesium atoms. (author)

  2. Effet de l'acide indole butyrique, de l'acide gibbérellique et d'un inhibiteur d'éthylène sur la fructification et la qualité des fruits du piment cultivé sous serre froide

    Directory of Open Access Journals (Sweden)

    Dridi, B.

    2005-01-01

    Full Text Available Effect of Indole Butyric Acid, Gibberellic Acid and an Ethylene Inhibitor on Fructification and Fruit Quality of Pepper Grown under Unheated Plastic House. The yield and fruit quality of pepper grown under unheated plastic house are usually negatively affected by low night temperature occurring during four to five months, this disrupt the local market supply and restrict the export possibilities. The effect of indole butyric acid (AIB, gibberellic acid (GA3 and an ethylene inhibitor (AgNO3 on fructification and fruit quality of two hot and two sweet pepper varieties grown under unheated plastic house, was studied. These substances, sprayed once per week just before flower initiation of the first four bifurcations, stimulated flower initiation and development; AgNO3 produced a significant increase in flower buds (98% more than the control and reduced the buds abortion. Treatments did not affect bud flower and flower abortion, but increased fruit characteristics; treatment with AIB produced the longest fruits and the highest number of seed per fruit. On Beldi, hot pepper variety, gibberellic acid and indole butyric acid treatment increased fruit soluble solid content, citric acid, ascorbic acid concentration and chlorophyll a content, while AgNO3 treatment increased chlorophyll b concentration.

  3. The cold monsters facing the climatic change. Trans-national states and actors in the political process bond to the climatic changes; Les monstres froids a l'epreuve du rechauffement de la planete. Etats et acteurs transnationaux dans le processus politique lie aux changements climatiques

    Energy Technology Data Exchange (ETDEWEB)

    Denis, B. [Universite Libre de Bruxelles (Belgium)

    2003-07-01

    The author discusses the evolution of the importance and the strategies of the different actors during the different phases of the political process and the learning of the state part in the international relations. (A.L.B.)

  4. Exclusive wearing of shoes of impregnated cloth by an adolescent girl during a cold winter: late effects in osseous tomo-scintigraphy and in magnetic resonance imaging; Froid hivernal et port exclusif de chaussures de sport en tissu par une adolescente: effets tardifs en tomoscintigraphie osseuse et en resonance magnetique nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    Als, C.; Marugg, S. [Medical Imaging Luzern, Lucerne (Switzerland); Als, C. [Zithaklinic, Service de Medecine Nucleaire, Grand-Duche de Luxembourg (Luxembourg)

    2009-10-15

    We present a rare clinical case of a 14-year-old adolescent girl from central Switzerland with an algoneurodystrophy of the left foot and leg 6 months after grade I frostbite(s) of the feet. After 6 months of constant pain of the digits, not attenuated by non steroid anti-inflammatory drugs, MRI of the feet reveals a predominantly left-sided and distal medullary edema, limited to the phalanges. Bone scintigraphy shows a predominantly left-sided diffuse feet hypoperfusion, coupled with an increased bone uptake of the left leg at late images. Inadequate life-style of adolescents, i.e., exclusively wearing shoes made of cloth and not of leather - even with temperatures below 0 Celsius degree in winter - might be a cause of chronic pain of foot digits. This is a rare demonstration of late effects of cold on foot digits by combined MRI and bone scintigraphy. Algoneurodystrophy in children and adolescents is an under diagnosed clinical entity. (authors)

  5. Control of the 133 cesium cold collisions, search for a variation of the fine structure constant using a dual rubidium-cesium atomic fountain; Controle des collisions froides du cesium {sup 133}Cs: tests de la variation de la constante de structure fine a l'aide d'une fontaine atomique double rubidium-cesium

    Energy Technology Data Exchange (ETDEWEB)

    Marion, H

    2005-03-15

    We developed a method of measurement of the frequency shift due to the collisions between cold atoms. This is the main systematic limitation for the accuracy of the Cs{sup 133} based fountains ({approx} 10{sup -15} in relative frequency). Consequently, we can measure this effect near 0.5% This opens prospects for improvements of the fountains performances in term of accuracy until 10{sup -16}. The fountain has also obtained a stability about 10{sup -14} at 1 s. We discovered for the first time, at very low magnetic field (5 {+-} 1 mG), Feshbach resonances. We also took a new absolute measurement of the hyperfine transition of the Rb{sup 87}, which is the most precise ever carried out and is used now as definition for the secondary standard. By comparing this value with those measured the previous years, we could carry out a test of the stability of the fine structure constant on the level of 10{sup -15} /yr. We led local comparisons between atomic fountains and the other fountains of the laboratory. Most stable it is unrolled with a combined stability of 5.10{sup -14} at 1 s. The behavior of the difference of the two clocks goes like white frequency noise up to 3.10{sup -16}. The assessment of the dual fountain accuracy budget has been evaluated at 7.10{sup -16} for the cesium part and 8.10{sup -16} for the rubidium part. We contributed to the realization of the scale of International Atomic Time, by series of calibrations of hydrogen masers. An atomic comparison of fountain by satellite links was tested between our laboratory and our German counterpart. This measurement has determined the good agreement between the two clocks. (author)

  6. High-temperature gas-cooled reactor technology development program. Annual progress report for period ending December 31, 1980

    Energy Technology Data Exchange (ETDEWEB)

    1981-08-01

    Research activities are described concerning HTGR chemistry; fueled graphite development; prestressed concrete pressure vessel development; structural materials; HTGR graphite studies; HTR core evaluation; reactor physics; shielding; application and project assessments; and HTR Core Flow Test Loop studies.

  7. Telomerase in (pre)neoplastic cervical disease

    NARCIS (Netherlands)

    Wisman, GBA; De Jong, S; Meersma, GJ; Helder, MN; Hollema, H; de Vries, EGE; Keith, WN; van der Zee, AGJ

    2000-01-01

    This study was performed to determine upregulation of the human telomerase RNA component (hTR) and mRNA of the catalytic subunit of telomerase (hTERT) in (pre)malignant cervical lesions, to analyze possible intralesional heterogeneity of hTR expression, and to relate hTR and hTERT mRNA levels to tel

  8. A novel screening system for secretion of heterologous proteins in Bacillus subtilis

    NARCIS (Netherlands)

    Trip, Hein; van der Veek, Patricia J.; Renniers, Ton C.; Meima, Rob; Sagt, Cees M.; Mohrmann, Lisette; Kuipers, Oscar P.

    2011-01-01

    High-level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two-component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive pr

  9. Telomerase in (pre)neoplastic cervical disease

    NARCIS (Netherlands)

    Wisman, GBA; De Jong, S; Meersma, GJ; Helder, MN; Hollema, H; de Vries, EGE; Keith, WN; van der Zee, AGJ

    2000-01-01

    This study was performed to determine upregulation of the human telomerase RNA component (hTR) and mRNA of the catalytic subunit of telomerase (hTERT) in (pre)malignant cervical lesions, to analyze possible intralesional heterogeneity of hTR expression, and to relate hTR and hTERT mRNA levels to tel

  10. Simulation 󈨔 Symposium.

    Science.gov (United States)

    1980-11-21

    Simulation directe par reseau electrique 4:30 - 4:45 p.m. CLOSING SESSION TECHNICAL APPLICATIONS Lecture Hall B Wednesday, June 25, 1980 vi ... . .I...Sensibilitd du moteur pas--pas au moyen d’un module a deux paramntres 11:10 - 11:30 aom. R. LeDoeuff / C. Iung (France) Digital Simulation of a Three Phase

  11. Development status and operational features of the high temperature gas-cooled reactor. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Winkleblack, R.K.

    1976-04-01

    The objective of this study is to investigate the maturity of HTR-technology and to look out for possible technical problems, concerning introduction of large HTR power plants into the market. Further state and problems of introducing and closing the thorium fuel cycle is presented and judged. Finally, the state of development of advanced HTR-concepts for electricity production, the direct cycle HTR with helium turbine, and the gas-cooled fast breeder is discussed. In preparing the study, both HTR concepts with spherical and block-type fuel elements have been considered.

  12. THE EFFECT OF PROSTAGLANDIN E1 ON PULMONARY BLOOD FLOW AFTER RETROGRADE FLUSH AND COLD STORAGE OF LUNGS

    Institute of Scientific and Technical Information of China (English)

    陈长志; 赵洪; 陆佩中

    2000-01-01

    ReSllm6 Objectif Nos studes Precedentes out montrd une panne fonCtion de la greffe pulmonaire traitde Prdalablementper perfusion forcde retrograde et un stockage d froid inns ~. L' etude Prdsente a pour but de determiner l' effet de ~ surlefiot mngUin du poumon trait4 Prdalablement per perfusion retrograde forcde et un stockage d froid. met~. 12poumons donneurs canins out ate trait4s per perfusion r4tFograde de solution UW. Chez 6 animaux du grouch A, 250ng furent injectes dans l' artrdre pulmonaire avant l...

  13. Effect of Various Environmental Stressors on Target Detection, Identification, and Marksmanship

    Science.gov (United States)

    2007-03-01

    diverses conditions environnementales difficiles, dont l’exposition à la chaleur et au froid, le bruit, l’exercice épuisant et la privation de sommeil ...stresseurs externes comme les extrêmes climatiques et des stresseurs internes comme la fatigue due à l’effort physique et à la privation de sommeil . Cela va...froid), de bruit sur le champ de bataille et de fatigue causée par l’effort physique et la privation de sommeil , avec consommation de caféine dans le

  14. Optical fibers installation the different technologies and the advantages of using the RTE power grid; Pose de fibres optiques les differentes technologies et les avantages lies a l'utilisation du reseau de transport d'electricite de RTE

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    To install optical fibers lines RTE implements two different technologies: the THYM line and the COE line. The two technologies are explained and compared with the advantages of using RTE for such an equipment. (A.L.B.)

  15. Extensions of I{sub X}T{sub E}T and its application to electric power network supervision; Extensions d`I{sub X}T{sub E}t pour son application a la supervision d`un reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    Despouys, O.; Ingrand, F.; Ghallab, M.; Gouyon, J.P.

    1997-07-01

    A chronicle model describes a set of possible behaviours for a given dynamic system. I{sub X}T{sub E}T uses a reified logic and temporal constraints to describe chronicles. It also uses algorithms which allow it to recognize all the instances of theses chronicles through a stream of time-stamped events given as an input. I{sub X}T{sub E}T is used in a number of complex supervision applications. Recent extensions to I{sub X}T{sub E}T, enabling its application at the national utility Electricite de France for electric network supervision and more especially for event and fault diagnosis. The new approach, notably based on facultative events or non-events, is compared to the FONSYNT previous supervision approach used at EDF

  16. Decision proposal aiming at creating an inquiry commission relative to the vulnerability of the power transportation and distribution network in France; Proposition de resolution visant a creer une commission d'enquete relative a la vulnerabilite du reseau de transport et de distribution d'electricite en france

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-02-15

    On January 24, 2009, a violent storm hit the SW of France and led to the collapse of the power network. The low- and medium-voltage lines, in particular, were seriously damaged. This vulnerability of the power grid is particularly ominous and has motivated this proposal of creation of an inquiry commission for: estimating the costs generated by the January 24, 2009 storm, evaluating the resistance capacity of power generation, transport and distribution networks in front of natural disasters or extreme climate conditions, calculating the average cots of the burial of the high- and very-high voltage power lines, studying the impact on health and on environment of aerial and buried very-high voltage power lines, elaborating a scenario of decentralization and diversification of power generation means, identifying the measures to be implemented in priority to significantly reduce the power consumption, and proposing the scheme of an emergency plan for extreme climate riskiness. (J.S.)

  17. An observer study for direct comparison of clinical efficacy of electronic to film portal images and their incorporation in a network; Apport de l'imagerie de haute energie dans un service de radiotherapie et incorporation de celle-ci dans un systeme en reseau

    Energy Technology Data Exchange (ETDEWEB)

    Pradier, O.; Hille, A.; Schmidberger, H.; Hess, C.F. [Gottingen Universitat, Klinik und Poliklinik fur Trahlentherapie und Radioonkologie (Germany)

    2001-06-01

    An observer study for direct comparison of clinical efficacy of electronic to film portal images and their incorporation in a network. To directly compare the clinical efficacy of electronic to film portal images and the advantages of comparing directly on the monitor the simulation image and the portal image. Material and methods. - This study was designed to compare clinical efficacy of electronic to film portal images acquired using a liquid matrix ion-chamber electronic portal imaging device (EPID) and a conventional film system. Two radiation oncologists served as observers and evaluated a total of 30 sets of images for three different treatment sites: lung, pelvis, and head/neck. Each set of images included a simulation image, a portal film, a video paper print of electronic portal images, and a video prints of electronic portal images. Four to six anatomical landmarks were selected from each treatment site. Each observer was asked to rate each landmark in terms of its clinical visibility and to rate the ease of making the pertinent verification decision in the corresponding electronic and film portal images with the aid of the simulation image. The time needed to obtain and analyse a conventional portal image and an EPID would be analysed for the radiotherapist and the medical technicians. Results- Ratings for the visibility of landmarks and for the verification decision of treatment ports were similar for electronic and film images for most landmarks. However, vertebral bodies and several landmarks in the pelvis such as the acetabulum and pubic symphysis were more visible in the electronic portal images than in the portal film images. For the medical technicians, the EPID is more comfortable, and they do not need to develop any images. Conclusion. -The visibility of landmarks in electronic portal images is comparable to that in film portal images. Verification of treatment ports based only on electronic portal images acquired using an electronic portal imaging device is generally achievable. Thus the integration of the EPID and simulation image in a network provides more flexibility in the daily work of a medical radiotherapy team. (authors)

  18. Adding a small hydroelectric power plant to the drinking water supply of the Liddes community. Feasibility study; Installation d'une petite centrale hydraulique sur le reseau d'eau potable de la commune de Liddes. Etude de faisabilite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    The actual drinking water supply no longer satisfies the needs of the Liddes community (southwestern Switzerland). This final report prepared for the Swiss Federal Office of Energy (SFOE) describes the results of a study for an enhanced water supply system including a small hydroelectric power plant. The authors propose to collect the water of two sources of the mountain torrent Aron and to use the considerable pressure difference for power generation. Four variants have been studied differing in the exact height difference, the flow rate and the location of the turbine building. The recommended variant delivers a nominal flow of 18 l/s at difference in height of 377 m to a Pelton turbine equipped with a single injector, resulting in an electrical power output of 54 kW. Electrical power production would amount to 281,000 kWh/year and the production cost to 0.126 CHF/kWh.

  19. SARNET european excellence network on nuclear reactor major accidents. Display and realizations after a year of operating; Sarnet reseau d'excellence europeen sur les accidents graves de reacteur nucleaire. Son deploiement et ses realisations apres une annee de fonctionnement

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-03-15

    The Sarnet (Severe Accident Research NETwork of excellence) is devoted to the research on major accidents of water cooled reactors. The developed knowledge will be integrated in a simulation tool ASTEC co-developed with the IRSN and the GRS. This evaluation report presents the context, the objectives and the program of the Sarnet network. It discusses the network operating and the ASTEC simulation code. Some examples of experimental programs are provided. (A.L.B.)

  20. The Curie laboratory. In the middle of a competence network. The study of radioactivity is an enterprise at multiple components; Le laboratoire Curie. Au coeur d`un reseau de competence. L`etude de la radioactivite est une entreprise a composantes multiples

    Energy Technology Data Exchange (ETDEWEB)

    Boudia, S. [Centre de recherche en histoire des sciences et des techniques a la Villette, 75 - Paris (France)

    1997-07-01

    After the first world war, the most important research laboratory is placed under the direction of a woman: Marie Curie. She leads a voluntarist policy to weave links with industry, the medical profession, and public authorities. In this network, circulate men, women, radium samples,calibration certificates, money, measuring instruments. (N.C.)

  1. Two strongly correlated electron systems: the Kondo mode in the strong coupling limit and a 2-D model of electrons close to an electronic topological transition; Deux systemes d'electrons fortement correles: le modele de reseau Kondo dans la limite du couplage fort et un modele bidimensionnel d'electrons au voisinage d'une transition topologique electronique

    Energy Technology Data Exchange (ETDEWEB)

    Bouis, F

    1999-10-14

    Two strongly correlated electron systems are considered in this work, Kondo insulators and high Tc cuprates. Experiments and theory suggest on one hand that the Kondo screening occurs on a rather short length scale and on the other hand that the Kondo coupling is renormalized to infinity in the low energy limit. The strong coupling limit is then the logical approach although the real coupling is moderate. A systematic development is performed around this limit in the first part. The band structure of these materials is reproduced within this scheme. Magnetic fluctuations are also studied. The antiferromagnetic transition is examined in the case where fermionic excitations are shifted to high energy. In the second part, the Popov and Fedotov representation of spins is used to formulate the Kondo and the antiferromagnetic Heisenberg model in terms of a non-polynomial action of boson fields. In the third part the properties of high Tc cuprates are explained by a change of topology of the Fermi surface. This phenomenon would happen near the point of optimal doping and zero temperature. It results in the appearance of a density wave phase in the under-doped regime. The possibility that this phase has a non-conventional symmetry is considered. The phase diagram that described the interaction and coexistence of density wave and superconductivity is established in the mean-field approximation. The similarities with the experimental observations are numerous in particular those concerning the pseudo-gap and the behavior of the resistivity near optimal doping. (author)

  2. La invalidez por causa psiquiátrica. Reseau Europeo di alternativa alia psíchiatria; Proyecto de Ley por la que se reforma en Italia la Ley de diciembre de 1978 relativa a la asistencia psiquiátrica.

    Directory of Open Access Journals (Sweden)

    Guillermo Rendueles Olmedo

    1984-01-01

    Full Text Available Las definiciones y la reforma de la condición legal de internado, las polémicas en torno alas pabellones de judiciales o el rechazo de las órdenes gubernativas de ingreso, han despertado con toda justicia honda resonancia en el campo psiquiátrico y así aparece reflejado en congresos, publicaciones y reuniones. Compartiendo esa perspectiva, quisiéramos introducir otro problema que ampliaría el tema de las libertades formales del enfermo psíquico, hacia otros aspectos legales, que se refieren a la condición de trabajador y a la imposibilidad de continuar dicha actividad en función de la propia enfermedad mental. Como ya veremos, el imposibilitado para continuar su trabajo por motivos psiquiátricos va a tener enormes dificultades para ser reconocido como inválido y protegido como tal con una pensión, y ello tanto por motivos que rozan la contradicción fundamental de las definiciones clásicas de psicosis endógenas -"Psicosis sin base orgánica demostrable"-como por motivos de concepción utilitarista y reparadora de fuerza de trabajo en el régimen general del INSALUD

  3. By-law from July 4, 2003, relative to the technical prescriptions of design and operation for the connection of a power generating facility to the public power transportation grid; Arrete du 4 juillet 2003 relatif aux prescriptions techniques de conception et de fonctionnement pour le raccordement au reseau public de transport d'une installation de production d'energie electrique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-09-01

    This by-law fixes the constructive and organisational dispositions that must fulfill the power generating facilities in order to make their connection to the public power grid conformable with the objectives of the decree from June 27, 2003. The dispositions concern: the voltage, connection scheme, protection systems, earthing, domain of operation, frequency adjustment and quality of current, communication and remote control systems of the power generating facility. (J.S.)

  4. Contract for access to the public power transportation network for an eligible consuming site. Particular conditions at the delivery point; Contrat d'acces au reseau public de transport d'electricte pour un site consommateur eligible. Conditions particulieres au point de livraison

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    On 1 November 2002, the new tariffs for the utilisation of the public power transmission and distribution networks, defined by decree no 2002-1014 of 19 July 2002, will come into effect. A new contract for access to the transmission network has been drawn up in co-operation with the Energy Regulation Commission (CRE) so as to be able to include these new tariffs. This new contract also takes into account the expectations expressed by the users of the transmission network concerning the energy delivery agreement used since the coming into effect of the European Directive on the opening of the electricity market in February 1999. On 31 October 2002, RTE is publishing a new version of the contract for access to the Public Transmission Networks. On 4 August 2003, RTE updated the particular conditions of the contract for access to the public transmission networks. This update takes into account the French regulator's deliberation dated 22/05/03, on the handling of eligible sites indirectly connected to public transmission systems. This document is a model form of the particular conditions of the contract. It comprises the following parts: purpose and contractual perimeter, connection to the public transportation network, metering and deductions, subscribed power, development, exploitation and maintenance of facilities, power continuity and quality, liability, tariffing and conditions of payment, declaration of the balancing actor, general dispositions. 7 models of forms of notification, demand, authorization, agreement, declaration etc.. are given in appendixes.

  5. Linking: Today's Libraries, Tomorrow's Technologies. Report of the Bibliographic and Communications Network Pilot Project. Canadian Network Papers Number 7 = Le Lien entre les Bibliotheques d'Aujourd'hui et les Technologies de Demain. Rapport du Projet Pilote du Reseau de Services Bibliographiques et de Communications. Documents sur les Reseaux Canadiens Numero 7.

    Science.gov (United States)

    National Library of Canada, Ottawa (Ontario).

    A pilot project was conducted from May 1980 to November 1983 to test the application of iNet--a decentralized, packet-switched telecommunications network--to bibliographic data interchange in Canada. The principal components of the project were participation of the Bibliographic Common Interest Group (BCIP), a group of libraries with stand-alone,…

  6. Communique: Special Issue on the International Network for Cooperation in Northern Science Created at a Meeting held in Edmonton, Alberta (October 12-15, 1982). Summary of Discussions and Agreements Reached = Numero special sur le Reseau Scientifique Internationale pour le Nord cree a la reunion tenue a Edmonton, Alberta (du 12 au 15 octobre 1982). Resume des discussions et accords conclus.

    Science.gov (United States)

    Communique, 1983

    1983-01-01

    Delegations from Canada, Finland, Greenland, Norway, Sweden, and the United States agreed to the establishment of a network for cooperation among individuals engaged in problems peculiar to the circumpolar North. The Northern Science Network, established within the Unesco Man and the Biosphere Program, consists of three themes: studies on the…

  7. The local-regional networks of radiation protection experts: a necessary tool to strengthen; Les reseaux de PCR: Les reseaux loco-regionaux de PCR: un outil necessaire a renforcer - La vie d'un reseau de PCR: l'APCRAP - Sud-Ouest

    Energy Technology Data Exchange (ETDEWEB)

    Barbey, P. [IMOGERE IBFA, Service de Radioprotection, Universite de Caen Basse-Normandie, 14 - Caen (France); Boirie, G. [Hopital Beaujon, 92 - Clichy (France); Barret, Ch. [GH Pitie-Salpetriere, Sevice Competent en Radioprotection, 75 - Paris (France); Barrey, N. [CHU de Bordeaux, 33 (France)

    2008-07-01

    After having recalled the evolution of the legal framework concerning radioprotection and the origin, role and training of the radiation protection experts (PCR in French for Personne Competente en Radioprotection), the author describes the difficulties faced by a PCR on a daily basis. Then, he outlines the interest of a development of local-regional networks between these professionals to promote information transmission and exchange, knowledge updating and so on, and even also to break the isolation of the PCR. He indicates the main objectives of these networks and evokes perspectives, notably after the professional meetings which have been and still are organized twice a year. Two additional and short papers briefly describe the life of one of these networks (the APCRAP, Association des personnes competentes en radioprotection de l'Assistance Publique, association of radiation protection experts belonging to public care services) and briefly present the objectives of the south-western network

  8. Network based on statistical multiplexing for event selection and event builder systems in high energy physics experiments; Reseau a multiplexage statistique pour les systemes de selection et de reconstruction d'evenements dans les experiences de physique des hautes energies

    Energy Technology Data Exchange (ETDEWEB)

    Calvet, D

    2000-03-01

    Systems for on-line event selection in future high energy physics experiments will use advanced distributed computing techniques and will need high speed networks. After a brief description of projects at the Large Hadron Collider, the architectures initially proposed for the Trigger and Data AcQuisition (TD/DAQ) systems of ATLAS and CMS experiments are presented and analyzed. A new architecture for the ATLAS T/DAQ is introduced. Candidate network technologies for this system are described. This thesis focuses on ATM. A variety of network structures and topologies suited to partial and full event building are investigated. The need for efficient networking is shown. Optimization techniques for high speed messaging and their implementation on ATM components are described. Small scale demonstrator systems consisting of up to 48 computers ({approx}1:20 of the final level 2 trigger) connected via ATM are described. Performance results are presented. Extrapolation of measurements and evaluation of needs lead to a proposal of implementation for the main network of the ATLAS T/DAQ system. (author)

  9. Study of sources terms and medical radioactivity transfer in the waste water system of the town of Toulouse; Etude des termes sources et du transfert de la radioactivite d'origine medicale dans le reseau d'assainissement de la ville de Toulouse

    Energy Technology Data Exchange (ETDEWEB)

    Debayle, C.

    2004-07-01

    It was demonstrated that the health establishments we studied implement means of radiation protection in accordance with the regulations and the recommendations of the circular D.G.S./D.H.O.S. in 2001. However, some exceeding of the average guide value recommended to the emissary of nuclear medicine services for iodine 131 were observed. The hypothesis we can suggest in view of the results is the recommendation to separate urines from feces induces the presence of iodine 131 because of the direct release of feces in the sewerage. On this base it would be interesting to consider the systematic storage of feces in tank at the nuclear medicine services exit, in conditions allowing to limit the nosocomial risk and to realize the earliest possible decay. In any event, it is important to survey these releases with devices for continuous measurement and not by point sampling to better understand the mechanisms. Finally, it might be worth exploring the possibilities of wastewater treatment through activated carbon cartridges and then limit the radioactive releases coming in the wastewater treatment plant. This would have for consequences to reduce the radioactivity transferred in the two units of muds storage and incineration. (N.C.)

  10. Hyperfunction of muscarinic receptor maintains long-term memory in 5-HT4 receptor knock-out mice.

    Directory of Open Access Journals (Sweden)

    Luis Segu

    Full Text Available Patients suffering from dementia of Alzheimer's type express less serotonin 4 receptors (5-HTR(4, but whether an absence of these receptors modifies learning and memory is unexplored. In the spatial version of the Morris water maze, we show that 5-HTR(4 knock-out (KO and wild-type (WT mice performed similarly for spatial learning, short- and long-term retention. Since 5-HTR(4 control mnesic abilities, we tested whether cholinergic system had circumvented the absence of 5-HTR(4. Inactivating muscarinic receptor with scopolamine, at an ineffective dose (0.8 mg/kg to alter memory in WT mice, decreased long-term but not short-term memory of 5-HTR(4 KO mice. Other changes included decreases in the activity of choline acetyltransferase (ChAT, the required enzyme for acetylcholine synthesis, in the septum and the dorsal hippocampus in 5-HTR(4 KO under baseline conditions. Training- and scopolamine-induced increase and decrease, respectively in ChAT activity in the septum in WT mice were not detected in the 5-HTR(4 KO animals. Findings suggest that adaptive changes in cholinergic systems may circumvent the absence of 5-HTR(4 to maintain long-term memory under baseline conditions. In contrast, despite adaptive mechanisms, the absence of 5-HTR(4 aggravates scopolamine-induced memory impairments. The mechanisms whereby 5-HTR(4 mediate a tonic influence on ChAT activity and muscarinic receptors remain to be determined.

  11. HTR1B, ADIPOR1, PPARGC1A, and CYP19A1 and Obesity in a Cohort of Caucasians and African Americans: An Evaluation of Gene-Environment Interactions and Candidate Genes

    Science.gov (United States)

    Edwards, Todd L.; Velez Edwards, Digna R.; Villegas, Raquel; Cohen, Sarah S.; Buchowski, Maciej S.; Fowke, Jay H.; Schlundt, David; Long, Ji Rong; Cai, Qiuyin; Zheng, Wei; Shu, Xiao-Ou; Hargreaves, Margaret K.; Jeffrey, Smith; Williams, Scott M.; Signorello, Lisa B.; Blot, William J.; Matthews, Charles E.

    2012-01-01

    The World Health Organization estimates that the number of obese and overweight adults has increased to 1.6 billion, with concomitant increases in comorbidity. While genetic factors for obesity have been extensively studied in Caucasians, fewer studies have investigated genetic determinants of body mass index (BMI; weight (kg)/height (m)2) in African Americans. A total of 38 genes and 1,086 single nucleotide polymorphisms (SNPs) in African Americans (n = 1,173) and 897 SNPs in Caucasians (n = 1,165) were examined in the Southern Community Cohort Study (2002–2009) for associations with BMI and gene × environment interactions. A statistically significant association with BMI survived correction for multiple testing at rs4140535 (β = −0.04, 95% confidence interval: −0.06, −0.02; P = 5.76 × 10−5) in African Americans but not in Caucasians. Gene-environment interactions were observed with cigarette smoking and a SNP in ADIPOR1 in African Americans, as well as between a different SNP in ADIPOR1 and physical activity in Caucasians. A SNP in PPARGC1A interacted with alcohol consumption in African Americans, and a different SNP in PPARGC1A was nominally associated in Caucasians. A SNP in CYP19A1 interacted with dietary energy intake in African Americans, and another SNP in CYP191A had an independent association with BMI in Caucasians. PMID:22106445

  12. Oxidation kinetics of innovative carbon materials with respect to severe air ingress accidents in HTRs and graphite disposal or processing; Oxidationskinetik innovativer Kohlenstoffmaterialien hinsichtlich schwerer Lufteinbruchstoerfaelle in HTR's und Graphitentsorgung oder Aufbereitung

    Energy Technology Data Exchange (ETDEWEB)

    Schloegel, Baerbel

    2010-07-01

    Currently future nuclear reactor concepts of the Fourth Generation (Gen IV) are under development. To some extend they apply with new, innovative materials developed just for this purpose. This thesis work aims at a concept of Generation IV Very High Temperature Reactors (VHTR) in the framework of the European project RAPHAEL (ReActor for Process heat, Hydrogen And ELectricity generation). The concept named ANTARES (AREVA New Technology based on advanced gas-cooled Reactors for Energy Supply) was developed by AEVA NP. It is a helium cooled, graphite moderated modular reactor for electricity and hydrogen production, by providing the necessary process heat due to its high working temperature. Particular attention is given here to oxidation kinetics of newly developed carbon materials (NBG-17) with still unknown but needed information in context of severe air ingress accident in VHTR's. Special interest is paid to the Boudouard reaction, the oxidation of carbon by CO{sub 2}. In case of an air ingress accident, carbon dioxide is produced in the primary reaction of atmospheric oxygen with reflector graphite. From there CO{sub 2} could flow into the reactor core causing further damage by conversion into CO. The purpose of this thesis is to ascertain if and to what degree this could happen. First of all oxidation kinetic data of the Boudouard reaction with NBG-17 is determined by experiments in a thermo gravimetric facility. The measurements are evaluated and converted into a common formula and a Langmuir-Hinshelwood similar oxidation kinetic equation, as input for the computer code REACT/THERMIX. This code is then applied to analyse severe air ingress accidents for several air flow rates. The results are discussed for two accident situations, in which a certain graphite burn off is achieved. All cases show much more damage to the graphite bottom reflector than to the reactor core. Thus the bottom reflector will lose its structural integrity much earlier than the core itself will be damaged by oxidation. (orig.)

  13. 高温堆停堆落球装置吸收球下落特性分析%Analysis of Falling Characteristics of Small Absorber Ball in HTR Shutdown Device

    Institute of Scientific and Technical Information of China (English)

    陈凤; 何学东; 李天津; 曹丽; 罗杰; 孙博; 黄志勇

    2013-01-01

    对高温气冷堆停堆落球装置中的吸收球下落特性进行研究.采用与实际吸收球等直径(φ6 mm)的玻璃球,以及与实际堆芯吸收球下落孔道等内径(φ60 mm)的有机玻璃管落球装置,进行空气环境中玻璃球的重力下落试验.孔口颗粒重力下落质量流率的经验公式Beverloo公式与本次试验结果符合较好,偏差在4%~12%.实测玻璃球在有机玻璃贮球罐中的安息角约为23°.试验结果表明:就目前所设计的带有锥形漏斗的贮球罐,对应于中心孔口垂直上方的玻璃球首先开始流动,然后周围的颗粒不断向孔口中心补充,玻璃球面逐渐下降,玻璃球在贮球罐内下落顺畅.

  14. R+D works for the further development of high temperature reactors. (1) Captive bearing experiments for active magnetic bearings. (2) Captive bearing test for HTR blowers. Final report 1.10.1983 - 31.3.1991; F+E-Arbeiten fuer die Weiterentwicklung von Hochtemperaturreaktoren. (1) Fanglagerversuche fuer aktive Magnetlager. (2) Fanglagertest fuer HTR-Geblaese. Abschlussbericht. 1.10.1983 - 31.3.1991

    Energy Technology Data Exchange (ETDEWEB)

    1991-03-14

    When using active magnetic bearings as blower shaft bearings, blower motors and bearings must be protected against mechanical damage in case of faults (example: total electrical supply failure due to the supply cables breaking). So-called captive bearings are provided, in order to be able to shut the blowers down safely in such faults. These captive bearings are roller bearings which are additionally fitted in the area of the blower shaft bearings, to prevent mechanical contact between the blower rotor and stator. As there was little experience available for the given boundary conditions, such as - speed, - acceleration, - bearing load, - bearing dimensions, - ambient conditions, appropriate development and tests had to be carried out. It was important to determine suitable captive bearings and the necessary ambient conditions, which will make it possible to support the failures of the magnetic bearings to be expected in 40 years` operation of the reactor without damage and to meet the requirements of the captive bearings. (orig./GL). [Deutsch] Beim Einsatz von aktiven Magnetlagern als Geblaesewellenlagerung sind Geblaesemotor und -lager vor mechanischer Schaedigung im Stoerfall (Beispiel: totaler Stromausfall durch Bruch der Versorgungskabel) zu schuetzen. Um bei derartigen Stoerfaellen die Geblaese sicher abfahren zu koennen, werden sogenannte Fanglager vorgesehen. Diese Fanglager sind Waelzlager, die im Bereich der Geblaesewellenlagerung zusaetzlich angebracht werden, um einen mechanischen Kontakt zwischen Geblaesemotor und -stator zu verhindern. Da fuer die vorgegebenen Randbedingungen, wie - Drehzahl, - Beschleunigung, - Lagerbelastung, - Lagerabmessungen, Umgebungsbedingungen nur geringe Erfahrungen vorlagen, mussten entsprechende Entwicklungen und Versuche durchgefuehrt werden. Hierbei galt es, geeignete Fanglager und die erforderlichen Umgebungsbedingungen zu ermitteln, die es ermoeglichen, die in 40 Betriebsjahren des Reaktors zu erwartenden Ausfaelle der Magnetlager und damit Anforderungen der Fanglager ohne Schaden zu ertragen. (orig./GL).

  15. Penser la guerre impérialiste

    Institute of Scientific and Technical Information of China (English)

    LiQiqing

    2005-01-01

    Depuis la guerre froide et surtout depuis le “11 septembre”, notre planète est tout le temps obsédée par le cauchemar des guerres impérialistes et du terrorisme. A la télévision on voit presque chaque jour des informations sur les attentats, les attentats-suicide,

  16. Missile Aerodynamics (Aerodynamique des Missiles)

    Science.gov (United States)

    1998-11-01

    guerre froide la production des missiles a baisse’, avec pour consequence une diminution des budgets de d6veloppement. Les nouveaux types de conflits ...Roma) Directeur - Gestion de l’information LUXEMBOURG (Recherche et developpement) - DRDGI 3 Voir Belgique Ministbre de la Difense nationale NORVEGE

  17. L'astronomie dans le monde

    OpenAIRE

    Manfroid, Jean

    2004-01-01

    Le réveil d'Huygens; à la recherche du rayonnement le plus froid; Les vagues monstrueuses qui coulent des navires sont repérées par les satellites radar de l'ESA; Exoplanète poids plume; Sinfoni à  l'ESO; TrES 1; Cassiopée A

  18. Technical feasibility and economics of tertiary oil recovery combined with hydrogen recovery from the resulting petroleum gas. Vol. 2. Assessment of product cost. Technische Machbarkeit und Wirtschaftlichkeit der kombinierten Tertiaeroelfoerderung mit Wasserstoffgewinnung aus dem anfallenden Erdoelgas. Bd. 2. Ermittlung der Produktkosten

    Energy Technology Data Exchange (ETDEWEB)

    1988-05-01

    The report investigates the use of HTR modular reactors for tertiary oil recovery in the Emsland oil fields of Ruehlermoor and Georgsdorf with a view to the economic aspects of such a project. The reference plant used for the study is a HTR-2 modular reactor for combined steam and hydrogen generation. In addition, some plant variants are considered (HTR-2 and HTR-3 modular systems) also for steam generation only, in this case providing for the plants' higher steam output to be fed to the oil field, or to be used otherwise without additional cost (e.g. for electricity generation). As the Emsland oil fields are sites where the conditions of application of HTR modular systems are relatively unfavourable, the study also covers an assessment of the economic potentials of larger HTR-4 modular systems and their application at more favourable (foreign) oil fields. (orig.).

  19. Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells.

    Science.gov (United States)

    Tomlinson, Rebecca L; Abreu, Eladio B; Ziegler, Tania; Ly, Hinh; Counter, Christopher M; Terns, Rebecca M; Terns, Michael P

    2008-09-01

    Telomere maintenance by telomerase is critical for the unlimited division potential of most human cancer cells. The two essential components of human telomerase, telomerase RNA (hTR) and telomerase reverse transcriptase (hTERT), are recruited from distinct subnuclear sites to telomeres during S phase. Throughout the remainder of the cell cycle hTR is found primarily in Cajal bodies. The localization of hTR to Cajal bodies and telomeres is specific to cancer cells where telomerase is active and is not observed in primary cells. Here we show that the trafficking of hTR to both telomeres and Cajal bodies depends on hTERT. RNA interference-mediated depletion of hTERT in cancer cells leads to loss of hTR from both Cajal bodies and telomeres without affecting hTR levels. In addition, expression of hTERT in telomerase-negative cells (including primary and ALT cancer cell lines) induces hTR to localize to both sites. Factors that did not stimulate hTR localization in our experiments include increased hTR RNA levels and Cajal body numbers, and expression of SV40 large T antigen and oncogenic Ras. Our findings suggest that the trafficking of telomerase to Cajal bodies and telomeres in cancer cells correlates with and depends on the assembly of the enzyme.

  20. Relations de Dispersion et Diffusion des Glueballs et des Mesons dans la Theorie de Jauge U(1)(2+1) Compacte

    Science.gov (United States)

    Ahmed, Chaara El Mouez

    Nous avons etudie les relations de dispersion et la diffusion des glueballs et des mesons dans le modele U(1)_{2+1} compact. Ce modele a ete souvent utilise comme un simple modele de la chromodynamique quantique (QCD), parce qu'il possede le confinement ainsi que les etats de glueballs. Par contre, sa structure mathematique est beaucoup plus simple que la QCD. Notre methode consiste a diagonaliser l'Hamiltonien de ce modele dans une base appropriee de graphes et sur reseau impulsion, afin de generer les relations de dispersion des glueballs et des mesons. Pour la diffusion, nous avons utilise la methode dependante du temps pour calculer la matrice S et la section efficace de diffusion des glueballs et des mesons. Les divers resultats obtenus semblent etre en accord avec les travaux anterieurs de Hakim, Alessandrini et al., Irving et al., qui eux, utilisent plutot la theorie des perturbations en couplage fort, et travaillent sur un reseau espace-temps.

  1. An Operational Implementation of a CBRN Sensor-Driven Modeling Paradigm for Stochastic Event Reconstruction

    Science.gov (United States)

    2010-05-01

    reconstruction de sources. Ce module permet d ’obtenir une evaluation rapide et robuste des parametres d’une source inconnue, au moyen d’un nombre ...au moyen d’un nombre determine de mesures de concentration de bruit obtenues a l’aide d’un reseau de capteurs. La methode mise en oeuvre dans le...planificateurs et les premiers repondants ( civils et militaires) de divers organismes federaux, provinciaux et municipaux aux fins de prise de decisions

  2. Functional Role of Serotonin in Insulin Secretion in a Diet-Induced Insulin-Resistant State

    Science.gov (United States)

    Kim, Kyuho; Oh, Chang-Myung; Ohara-Imaizumi, Mica; Park, Sangkyu; Namkung, Jun; Yadav, Vijay K.; Tamarina, Natalia A.; Roe, Michael W.; Philipson, Louis H.; Karsenty, Gerard; Nagamatsu, Shinya

    2015-01-01

    The physiological role of serotonin, or 5-hydroxytryptamine (5-HT), in pancreatic β-cell function was previously elucidated using a pregnant mouse model. During pregnancy, 5-HT increases β-cell proliferation and glucose-stimulated insulin secretion (GSIS) through the Gαq-coupled 5-HT2b receptor (Htr2b) and the 5-HT3 receptor (Htr3), a ligand-gated cation channel, respectively. However, the role of 5-HT in β-cell function in an insulin-resistant state has yet to be elucidated. Here, we characterized the metabolic phenotypes of β-cell-specific Htr2b−/− (Htr2b βKO), Htr3a−/− (Htr3a knock-out [KO]), and β-cell-specific tryptophan hydroxylase 1 (Tph1)−/− (Tph1 βKO) mice on a high-fat diet (HFD). Htr2b βKO, Htr3a KO, and Tph1 βKO mice exhibited normal glucose tolerance on a standard chow diet. After 6 weeks on an HFD, beginning at 4 weeks of age, both Htr3a KO and Tph1 βKO mice developed glucose intolerance, but Htr2b βKO mice remained normoglycemic. Pancreas perfusion assays revealed defective first-phase insulin secretion in Htr3a KO mice. GSIS was impaired in islets isolated from HFD-fed Htr3a KO and Tph1 βKO mice, and 5-HT treatment improved insulin secretion from Tph1 βKO islets but not from Htr3a KO islets. Tph1 and Htr3a gene expression in pancreatic islets was not affected by an HFD, and immunostaining could not detect 5-HT in pancreatic islets from mice fed an HFD. Taken together, these results demonstrate that basal 5-HT levels in β-cells play a role in GSIS through Htr3, which becomes more evident in a diet-induced insulin-resistant state. PMID:25426873

  3. Genetic Variation and the Risk of Haloperidol-Related Parkinsonism in Elderly Patients A Candidate Gene Approach

    NARCIS (Netherlands)

    Knol, Wilma; van Marum, Rob J.; Jansen, Paul A. F.; Strengman, Eric; Al Hadithy, Asmar F. Y.; Wilffert, Bob; Schobben, Alfred F. A. M.; Ophoff, Roel A.; Egberts, Toine C. G.

    2013-01-01

    Factors that influence the variation in occurrence of antipsychotic-related parkinsonism in elderly have not been well elucidated. The aim of this study was to investigate whether previous identified and studied genetic polymorphisms at DRD2, ANKK1, DRD3, HTR2A, HTR2C, RGS2, COMT, and BDNF genes are

  4. Pharmacogenetics of parkinsonism, rigidity, rest tremor, and bradykinesia in African-Caribbean inpatients : Differences in association with dopamine and serotonin receptors

    NARCIS (Netherlands)

    Al Hadithy, Asmar F.; Wilffert, Bob; Stewart, Roy E.; Looman, Nicole M.; Bruggeman, Richard; Brouwers, Jacobus R.; Matroos, Glenn E.; van Os, Jim; Hoek, Hans W.; van Harten, Peter N.

    2008-01-01

    We studied the association between polymorphisms of genes coding for dopamine D-2 (DRD2), dopamine D-3 (DRD3), serotonin 2(a) (HTR2A), and serotonin 2(c) (HTR2C) receptors and Antipsychotic-Induced Parkinsonism (AIP), rigidity, bradykinesia, and rest-tremor in African-Caribbeans treated with antipsy

  5. TNF receptor-associated factor-2 binding site is involved in TNFR75-dependent enhancement of TNFR55-induced cell death

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    TNF recepter-55 is the main mediator of TNF-induced apoptosis. TNF receptor-75-dependent induction or enhancement of cytotoxicity has been explained by intracellular signaling, “ligand passing”, or induction of endogenous TNF. To study the function of human TNF receptor-75 (hTR75) and the interaction between human TNF receptor-55 (hTR55) and hTR75 in hTNFc-induced cytotoxicity, Hep-2 cells were transfected with bicistronic expression vector of hTR75 and its deletion mutants genes. hTNFα-induced cytotoxicity was determined by crystal violet colorimetric method. The expression of hTR75 and its deletion mutants in Hep-2 cells was demonstrated by RT-PCR and indirect ELISA. We found that the overexpressed hTR75 could significantly increase the susceptibility of Hep-2 cells to hTNFα which especiαlly required TRAF2 binding site. hTR75 could not only mediate partial hTNFα-induced cytotoxicity independently but also fulfill an accessory role in enhancing or synergizing hTR55-mediated cytotoxicity.

  6. Control rod drive for high temperature gas cooled reactor

    Institute of Scientific and Technical Information of China (English)

    DengJun-Xian; XuJi-Ming; 等

    1998-01-01

    This control rod drive is developed for HTR-10 high temperature gas cooled test reactor.The stepmotor is prefered to improve positioning of the control rod and the scram behavior.The preliminary test in 1600170 ambient temperature shows that the selected stepmotor and transmission system can meet the main operation function requirements of HTR-10.

  7. Clinical response to antipsychotic drug treatment : Association study of polymorphisms in six candidate genes

    NARCIS (Netherlands)

    Vehof, Jelle; Burger, Huibert; Wilffert, Bob; Al Hadithy, Asmar; Alizadeh, Behrooz Z.; Snieder, Harold

    Pharmacogenetic studies have demonstrated significant associations between several candidate genes (DRD2, DRD3, 5HTR2A and 5HTR2C, COMT and MTHFR) and antipsychotic drug response. The present study investigates the effect of nine polymorphisms in these genes for an association with antipsychotic

  8. Decision from the Commission of Electricity Regulation (CRE) dated from May 2, 2002 about a dispute between the manager of the power transportation network (RTE) and the autonomous administration of Parisian transports (RATP) relative to the conditions of consideration of the multiplicity of power supply points in the contractual system of access to the power grid; Decision de la Commission de Regulation de l'Electricite (CRE) en date du 2 mai 2002 sur un different qui oppose le gestionnaire du Reseau de Transport d'Electricite (RTE) et la Regie Autonome des Transports Parisiens (RATP) relatif aux conditions de prise en compte de la multiplicite des points de livraison dans le dispositif contractuel d'acces au reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-05-01

    This document is the analysis made by the French commission of electricity regulation (CRE) about a dispute between the French manager of the power transportation grid (RTE) and the autonomous administration of Parisian transports (RATP) about its contract of access to the grid for the power supply of the underground railway of Paris. The RATP requested a regrouping of all its 7 supply points in a single contract. After analysis of the hearings, the CRE considers that no discrimination exists in the contractual system proposed by the RTE and thus the RATP request has been rejected. (J.S.)

  9. Decision from the Commission of Electricity Regulation (CRE) dated from December 12, 2002 about a disagreement between PEM Abrasif Refractaires (PEMAR) company and the Power transportation Network (RTE), as manager of the public power transportation grid, relative to the tariffing of public networks use applicable to the consumers connected in 42 kV voltage; Decision de la Commission de Regulation de l'Electricite (CRE) en date du 12 decembre 2002 sur un different qui oppose la societe PEM Abrasif Refractaires (PEMAR) a Reseau de Transport d'Electricite (RTE), en tant que gestionnaire du reseau public de transport d'electricite, relatif a la tarification d'utilisation des reseaux publics applicable aux consommateurs raccordes en tension 42 kV

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-12-01

    This document presents the analysis made by the French commission of electricity regulation (CRE) about a high voltage tariff disagreement between PEM Abrasif Refractaires (PEMAR) company (La Defense, Courbevoie France) and RTE, the French manager of the power transportation grid. PEMAR company claims for a contract of power supply tariff conformable with the application of the decree from July 19, 2002 which, after comparison with the previous decrees, contains a mistake in the definition of voltage classes. (J.S.)

  10. Decision from the Commission of Electricity Regulation (CRE) dated from June 27, 2002 about a disagreement between Semmaris company and Electricite de France, as manager of the public power distribution grid, relative to the conditions of consideration of the multiplicity of delivery points in the contractual system of access to the grid; Decision de la Commission de Regulation de l'Electricite (CRE) en date du 27 juin 2002 sur un different, qui oppose la societe Semmaris a Electricite de France, en tant que gestionnaire du reseau public de distribution d'electricite, relatif aux conditions de prise en compte de la multiplicite des points de livraison dans le dispositif contractuel d'acces au reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-06-01

    This document presents the analysis made by the French commission of electricity regulation (CRE) about a disagreement between Semmaris company (Rungis, France) and Electricite de France (EdF) concerning the request by Semmaris for the subscription of a single contract of access to the grid for its 18 supply points instead of 9 contracts as proposed by EdF after acceptation of a partial regrouping of some supply points under the same contracts. After examination of the hearings, the CRE requested EdF to establish non-discriminatory objective criteria for the regrouping of different delivery points in a same contract before making a new examination of the disagreement between Semmaris and EdF. (J.S.)

  11. Energy, institutions and development: rightfulness and institutional conditions of a spreading of renewable energies in developing countries. The example of power generation for networks; Rapport de stage et memoire de DESS. Energie, institutions et developpement: legitimite et conditions institutionnelles d'une