WorldWideScience

Sample records for research note micropropagation

  1. Research Note:

    DEFF Research Database (Denmark)

    Behuria, Pritish; Buur, Lars; Gray, Hazel

    2017-01-01

    its core conceptual and methodological features. This Research Note starts by setting out our understanding of political settlements and provides an overview of existing political settlements literature on African countries. The note then explores how the key concept of ‘holding power’ has been...

  2. RESEARCH NOTE

    Indian Academy of Sciences (India)

    Navya

    RESEARCH NOTE. CDKN2A and MC1R ... Department of Pharmacy and Department of Nursing, School of Health Sciences, Frederick. University, Nicosia ..... Appears with highest frequency in African, Asian-Indian, and Papua. New Guinean ...

  3. Gerbera micropropagation.

    Science.gov (United States)

    Cardoso, Jean C; Teixeira da Silva, Jaime A

    2013-12-01

    Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year. Numerous types of explants and protocols for micropropagation have been established and used for gerbera. Shoot tips are the commonly used explant while adventitious shoot induction from the capitulum is also a popular method. Most papers in the literature have focused on testing the influence of different types and combinations of plant growth regulators with the aim of improving the regeneration and multiplication stage of one or few cultivars. Genotype is one of the most influential factors on the response of gerbera in vitro. Despite this, no successful universal protocol has yet been developed for multiple cultivars, limiting the usefulness of current protocols for commercial biotechnology labs. Slow-growing endogenous bacteria are one of the most important problems in gerbera micropropagation but require more studies on control and prevention. Individual shoots are normally easy to root, usually in excess of 90% of plantlets, but the acclimatization stage requires improvements and new technologies to increase the survival of plants. Epigenetic variations in micropropagated gerbera are frequently observed only with high concentrations of cytokinins in the culture medium but somaclonal variation is rare. Copyright © 2013 Elsevier Inc. All rights reserved.

  4. Revisiting convergence: A research note.

    Science.gov (United States)

    Clark, Rob

    2015-09-01

    A number of recent studies show that income inequality is declining between countries. In this research note, I question the significance of this trend by examining the role of initial conditions in producing convergence. An important (but neglected) property of inequality dynamics is the tendency for extreme distributions to become more moderate. When income disparities are large, the subsequent trend is biased toward convergence. Conversely, when initial conditions approach parity, divergence becomes the more likely long-term outcome. I apply this principle to trends in GDP PC across 127 countries during the 1980-2010 period. Using counterfactual analysis, I manipulate the initial level of inequality in GDP PC while holding constant each country's observed growth rate during the sample period. I find that the growth dynamics of GDP PC produce either convergence or divergence based simply on the initial distribution of income. The point of transition occurs at a moderate level of inequality, whether using population weights (Gini=.365) or not (Gini=.377). I conclude that the recent convergence observed in GDP PC is primarily a function of large income gaps between countries and would not have materialized at more moderate levels of initial inequality. By contrast, an examination of the pre-1950 period reveals divergent growth patterns that are not sensitive to initial conditions. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Micropropagation of banana.

    Science.gov (United States)

    Kaçar, Yıldız Aka; Faber, Ben

    2012-01-01

    Banana (Musa spp. AAA) is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation. Conventional micropropagation techniques, however, may be too costly for commercial purposes. Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana.

  6. Stalin and Marxism: a research note

    NARCIS (Netherlands)

    van Ree, E.

    1997-01-01

    This article concerns the research done by the author in Stalin‘s private library. The notes made in the works of Marx, Engels and Lenin suggest that until the end of his life Stalin felt himself in general agreement with these "classics." The choice of books and the notes support the thesis that,

  7. Coping with Stress. Research Notes.

    Science.gov (United States)

    Jordan, Debra J.

    1995-01-01

    Research related to the impact of exercise on stress indicates that a regular aerobic exercise program is important to control the negative effects of stress. It was also reported that those who are physically fit have higher levels of self-esteem. Implications for camp staff involve starting a regular exercise program to offset job-related…

  8. and popular organization (research notes

    Directory of Open Access Journals (Sweden)

    Paulo J. Krischke

    2006-01-01

    Full Text Available This paper discusses some interfaces between the approaches to political learning, and their reference to situations of social exclusion, conflict and popular organization. The first part of the paper discusses the approach to the study of political learning among the elites; the second part examines approaches to research of political culture among the masses; and the third part outlines an alternative approach to political learning derived from Jürgen Habermas’s theory of “communicative action”. In the paper these approaches are applied to the study of a territory of exclusion, conflict, and popular organisation (a group of favelas in downtown Florianópolis, Santa Catarina, Brazil. Finally, the paper outlines some procedures to achieve a more general understanding of political learning

  9. Micropropagation of apple--a review.

    Science.gov (United States)

    Dobránszki, Judit; da Silva, Jaime A Teixeira

    2010-01-01

    Micropropagation of apple has played an important role in the production of healthy, disease-free plants and in the rapid multiplication of scions and rootstocks with desirable traits. During the last few decades, in apple, many reliable methods have been developed for both rootstocks and scions from a practical, commercial point of view. Successful micropropagation of apple using pre-existing meristems (culture of apical buds or nodal segments) is influenced by several internal and external factors including ex vitro (e.g. genotype and physiological state) and in vitro conditions (e.g., media constituents and light). Specific requirements during stages of micropropagation, such as the establishment of in vitro cultures, shoot multiplication, rooting of microshoots and acclimatization are summarized in this review. New approaches for increasing shoot multiplication and rooting for apple and current use of micropropagated plantlets as tools in basic and applied research are also discussed.

  10. Automatic micropropagation of plants

    Science.gov (United States)

    Otte, Clemens; Schwanke, Joerg; Jensch, Peter F.

    1996-12-01

    Micropropagation is a sophisticated technique for the rapid multiplication of plants. It has a great commercial potential due to the speed of propagation, the high plant quality, and the ability to produce disease-free plants. However, micropropagation is usually done by hand which makes the process cost-intensive and tedious for the workers especially because it requires a sterile work-place. Therefore, we have developed a prototype automation system for the micropropagation of a grass species (miscanthus sinensis gigantheus). The objective of this paper is to describe the robotic system in an overview and to discuss the vision system more closely including the implemented morphological operations recognizing the cutting and gripping points of miscanthus plants. Fuzzy controllers are used to adapt the parameters of image operations on-line to each individual plant. Finally, we discuss our experiences with the developed prototype an give a preview of a possible real production line system.

  11. Aromatic cytokinins in micropropagated potato plants

    Czech Academy of Sciences Publication Activity Database

    Baroja, F. E.; Aguirreolea, J.; Martínková, Hana; Hanuš, Jan; Strnad, Miroslav

    2002-01-01

    Roč. 40, č. 3 (2002), s. 217-224 ISSN 0981-9428 R&D Projects: GA MŠk OC 844.10; GA ČR GA301/02/0475 Institutional research plan: CEZ:AV0Z5038910 Keywords : Acclimatization * Aromatic cytokinins * Micropropagation Subject RIV: CE - Biochemistry Impact factor: 1.582, year: 2002

  12. Note Taking on Trial: A Legal Application of Note-Taking Research

    Science.gov (United States)

    Kiewra, Kenneth A.

    2016-01-01

    This article is about note taking, but it is not an exhaustive review of note-taking literature. Instead, it portrays the application of note-taking research to an unusual and important area of practice--the law. I was hired to serve as an expert witness on note taking in a legal case that hinged, in part, on the completeness and accuracy of…

  13. Financial accounting: an epistemological research note

    Directory of Open Access Journals (Sweden)

    Eduardo Schiehll

    2007-12-01

    Full Text Available This research note is the result of the authors' reflections on epistemological issues in respect to the financial accounting field. From an epistemological perspective, this document attempts to trace the philosophical, historical, sociological, and discursive research perspectives that have guided academic research in the field of financial accounting. In order to do so, this document explores the distinctions and connections between accounting theory and accounting practice, which we believe is the first step towards understanding accounting as a scientific discipline. We analyze the theories underpinning financial accounting research, discussing its purposes, historic evolution, and scientific methods used. This document also discuss the sociological and discursive contexts of financial accounting in order to demonstrate that, like every other social science, accounting research is based upon assumptions about the nature of it players, or social networks. This document does not have the pretension to cover or close the discussion about all the pitfalls of this complex topic. In this sense, we try to document our analysis and draw some arguments in order to offer evidence for further discussion.Este ensaio teórico é resultado de reflexões dos autores acerca de questões epistemológicas sobre a contabilidade financeira. Através de uma perspectiva epistemológica, este trabalho busca traçar perspectivas filosóficas, históricas, sociológicas e discursivas que guiaram a pesquisa acadêmica na área da contabilidade financeira. Para isso, exploram-se as similaridades e diferenças entre a teoria e a prática contábil, pois esse é o primeiro para a compreensão da contabilidade como disciplina científica. Analisam-se as teorias que suportam a pesquisa em contabilidade financeira, discutindo seu propósito, evolução histórica e métodos científicos utilizados. O presente trabalho também discute os contextos sociológicos e

  14. Notes on Commercialization of Biotechnology Research

    DEFF Research Database (Denmark)

    Høy Jakobsen, Palle

    This book introduces aspects of commercialization of biomedical & biotech research including pharmaceutical drug and medical device development, innovation concepts, patent protection & commercialisation of research by securing investments in new companies and by licensing of the commersialisatio...

  15. RESEARCH NOTE Molecular genetic analysis of consanguineous ...

    Indian Academy of Sciences (India)

    Navya

    Molecular genetic analysis of consanguineous families with primary microcephaly ... Translational Research Institute, Academic Health System, Hamad Medical ..... bridging the gap between homozygosity mapping and deep sequencing.

  16. Clinical Psychology and Research: epistemological notes

    Directory of Open Access Journals (Sweden)

    Emanuela Coppola

    2013-05-01

    Full Text Available The paper proposes a reflection on the relationship between clinical psychology and research, highlighting the constant epistemological crossing the two practices, empirical and professional. The paper warns against the pitfalls of reductionism that, in both cases, may impact the effectiveness of therapeutic results. In fact, both in clinical practice and is in psychological research, the mere application of techniques contradicts the specificity of the object of study (the mind which, rather, requires the constant attention to a complexity of variables and contextual elements essential for the understanding the psychic. Qualitative research has been a prolific space for dialogue and joint trials between research and clinical practice that has rehabilitated scientific dignity of affective and subjective for a long time confined to the ephemeral world of poetry and literature. It must therefore be a further extension of the convergence not only of qualitative and quantitative methods but also of training modules for researchers and practitioners are able to stimulate, in daily practice, confidence in the utility of scientific monitoring and detection of inter-subjective variables in research devices.

  17. Dendrobium micropropagation: a review.

    Science.gov (United States)

    da Silva, Jaime A Teixeira; Cardoso, Jean Carlos; Dobránszki, Judit; Zeng, Songjun

    2015-05-01

    Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review. Not only will this expand the use for mass propagation, but will also allow for the conservation of important germplasm. Information on the in vitro responses of Dendrobium for developing efficient protocols for breeding techniques based on tissue culture, such as polyploidization, somatic hybridization, isolation of mutants and somaclonal variants and for synthetic seed and bioreactor technology, or for genetic transformation, is discussed in this review. This is the first such review on this genus and represents half a decade of literature dedicated to Dendrobium micropropagation.

  18. Call for concept notes for research on gender equality and ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2017-08-07

    Aug 7, 2017 ... Call for concept notes for research on gender equality and decentralization in Myanmar ... address gender inequality in political participation; and; improve gender-sensitive ... ROSSA's latest bulletin puts a focus on women.

  19. Research Note: Comparative antibacterial activities of oil-palm ...

    African Journals Online (AJOL)

    Research Note: Comparative antibacterial activities of oil-palm Elaeis ... The antimicrobial activities liquid pyrolysates (obtained by destructive distillation), their ... respective chloroform fractions which showed higher activities than the crude ...

  20. Environmental accounting and reporting of energy utility companies. Research notes

    International Nuclear Information System (INIS)

    Heiskanen, E.; Heininen, M.; Heurlin, E.; Lovio, R.; Paenkaelaeinen, M.

    1997-09-01

    The research note consists of articles written by a number of authors. The aim of the articles is to describe general development trends of environmental accounting and reporting from the point of view of the energy sector

  1. Microinitiation, micropropagation and damage

    Energy Technology Data Exchange (ETDEWEB)

    Savalle, S.; Cailletaud, G. (ONERA, 75 - Paris (France))

    It is now well known that, on the microscopic scale, the fatigue failure process can be partitioned in two main stages: microinitiation and micropropagation. We show here the advantage in introducing these two successive steps in life prediction models for high temperature fatigue and creep. In a first part, we show that there is a non-interaction field between these two phenomena. Then, an extensive application is made using data on the Nickel-base alloy IN100: for some tests, creep and fatigue interact simultaneously, and for some others, they act consecutively. Finally, the same concepts are applied to the case of a loading corresponding to the superposition of vibrations and a large mean stress.

  2. Micropropagation of Rubus spp.

    Directory of Open Access Journals (Sweden)

    Åke Finne

    1986-09-01

    Full Text Available Rapid micropropagation of ‘Black Satin’, ‘Thornless Evergreen’ and ‘Bedford Giant’ was achieved by culturing shoot tips in a 1/1 MS. The best medium in the introduction stage contained 2.0 mg/l BAP. Shoot proliferation occurred with 3.0 mg/1BAP. Some problems with callus and phenolic compounds appeared in both introduction and shoot multiplication stages. The plantlets were rooted either in 1/10 MS without hormones or directly in peat. Two months later most of ‘Black Satin’ and ‘Bedford Giant’ and about half of ‘Thornless Evergreen’ were growing steadily. Theoretically it is possible to produce 60,000 plants within a half year by this method.

  3. Micropropagation of Iris sp.

    Science.gov (United States)

    Jevremović, Slađana; Jeknić, Zoran; Subotić, Angelina

    2013-01-01

    Irises are perennial plants widely used as ornamental garden plants or cut flowers. Some species accumulate secondary metabolites, making them highly valuable to the pharmaceutical and perfume industries. Micropropagation of irises has successfully been accomplished by culturing zygotic embryos, different flower parts, and leaf base tissues as starting explants. Plantlets are regenerated via somatic embryogenesis, organogenesis, or both processes at the same time depending on media composition and plant species. A large number of uniform plants are produced by somatic embryogenesis, however, some species have decreased morphogenetic potential overtime. Shoot cultures obtained by organogenesis can be multiplied for many years. Somatic embryogenic tissue can be reestablished from leaf bases of in vitro-grown shoots. The highest number of plants can be obtained by cell suspension cultures. This chapter describes effective in vitro plant regeneration protocols for Iris species from different types of explants by somatic embryogenesis and/or organogenesis suitable for the mass propagation of ornamental and pharmaceutical irises.

  4. Reducing Bias: Research Notes on Racism in America.

    Science.gov (United States)

    Mabbutt, Richard

    This paper highlights recent developments in research on racism in the United States, and notes several conceptual issues of significance for the long-range planning work of those interested in reducing racism in America and particularly in Idaho. Growth in the number of minority researchers has resulted in increased attention toward racism as it…

  5. Research Note: Inside an Indonesian Online Library for Radical Materials

    Directory of Open Access Journals (Sweden)

    Muhammad Haniff Hassan

    2012-12-01

    Full Text Available This Research Note provides a review of an Indonesian online library for radical materials. The objective of this review is to compile data and information that will contribute to the understanding of the online radicalisation phenomenon as well as the extremists themselves. Based on data found on the online library, this Research Note reports findings on the influence of Al-Maqdisi’s website; the emphasis on translation work of Arabic materials to Indonesian language by radicals and the value of Arabic materials to them. It also covers influential thinkers and ideologues and the use of the Wikipedia modus operandi to hasten the development of the website and effect mobilisation and recruitment, among others things. Based on the data found, this Research Note concludes that ideas matter to radicals.

  6. Noted

    Science.gov (United States)

    Nunberg, Geoffrey

    2013-01-01

    Considering how much attention people lavish on the technologies of writing--scroll, codex, print, screen--it's striking how little they pay to the technologies for digesting and regurgitating it. One way or another, there's no sector of the modern world that is not saturated with note-taking--the bureaucracy, the liberal professions, the…

  7. Research Note On New Limits of the Coefficient of Gravitation ...

    Indian Academy of Sciences (India)

    Research Note. On New Limits of the Coefficient of Gravitation Shielding. Michele Caputo. Physics Department, University La Sapienza, Piazzale A. Moro 2, 00185 Roma, Italy. and. Department of Geology and Geophysics, Texas A&M University, College Station TX, 77843,. USA. e-mail: mcaputo@g24ux.phys.uniroma1.it.

  8. Research Note Nutritional evaluation of Ficus thonningii Blume ...

    African Journals Online (AJOL)

    Research Note Nutritional evaluation of Ficus thonningii Blume leaves as ruminant livestock feed in the Ahferom district of Tigray, Ethiopia. ... Data on foliar nitrogen (N), phosphorus (P), potassium (K), dry matter (DM), crude protein (CP), digestible crude protein (DCP), ether extract (EE), crude fibre (CF), ash content and ...

  9. Research Note Identifying key grazing indicators to monitor trends in ...

    African Journals Online (AJOL)

    Research Note Identifying key grazing indicators to monitor trends in the veld condition of Lambert's Bay Strandveld, South Africa. ... from which a minimum number of species necessary to monitor trends in the condition of the veld were determined, making it user-friendly for land-users, extension officers and others. The key ...

  10. Micropropagation of rose cultivar 'Pareo'

    African Journals Online (AJOL)

    Jane

    2011-08-22

    Aug 22, 2011 ... Serik Mukhambetzhanov*, Svetlana Nam, Valentina Mursalieva and Izbasar Rakhimbaev. Laboratory of Clonal Propagation, Institute of Plant Biology and Biotechnology, 050040 Almaty, Kazakhstan. Accepted 1 July, 2011. A protocol was developed for micropropagation of rose cv. 'Pareo'. Nodal segments ...

  11. Nemesia root hair response to paper pulp substrate for micropropagation.

    Science.gov (United States)

    Labrousse, Pascal; Delmail, David; Decou, Raphaël; Carlué, Michel; Lhernould, Sabine; Krausz, Pierre

    2012-01-01

    Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia roots growing in agar or in paper-pulp substrates were evidenced through scanning electron microscopy. Roots developed in agar have shorter hairs, larger rhizodermal cells, and less organized root caps than those growing on paper pulp. In conclusion, it should be noted that in this study, in vitro microporous substrates such as paper pulp lead to the production of similar root hairs to those found in greenhouse peat substrates. Consequently, if agar could be used for micropropagation, rooting, and plant acclimatization, enhancement could be achieved if rooting stage was performed on micro-porous substrates such as paper pulp.

  12. Note

    DEFF Research Database (Denmark)

    Kullman, Mikael; Campillo, Javier; Dahlquist, Erik

    2016-01-01

    Globally, more than 50% of all people are living in cities today. Enhancing sustainability and efficiency of urban energy systems is thus of high priority for global sustainable development. The European research project PLEEC (Planning for Energy Efficient Cities) focuses on technological...

  13. Micropropagation of Plantago camtschatica Link

    Directory of Open Access Journals (Sweden)

    Emilia Andrzejewska-Golec

    2011-01-01

    Full Text Available The Far East medicinal plant - Plantago camtschatica was propagated in vitro from tips of shoots (obtained in vitro and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots, first leaves. To our knowledge there is no information in literature about in vitro culture of this plantain. MS basal medium, supplemented with 0.6 pM IAA in combination with various cytokinins (BA, KIN, ZEA, was used. After 6 weeks of culture, micropropagation rate (MR - mean number of buds and shoots per explant - was calculated. Our study proved that P. camtschatica species was amenable to propagation in vitro from different kinds of explants. However, multiplication by adventitious shoot regeneration from hypocotyl explants was found to be the most suitable method for the propagation of this plant. Adventitious shoots could root without stimulation what allows to omit the stage of rooting. The plants obtained as a result of micropropagation were not phenotypically changed.

  14. Research Note on the Energy Infrastructure Attack Database (EIAD

    Directory of Open Access Journals (Sweden)

    Jennifer Giroux

    2013-12-01

    Full Text Available The January 2013 attack on the In Amenas natural gas facility drew international attention. However this attack is part of a portrait of energy infrastructure targeting by non-state actors that spans the globe. Data drawn from the Energy Infrastructure Attack Database (EIAD shows that in the last decade there were, on average, nearly 400 annual attacks carried out by armed non-state actors on energy infrastructure worldwide, a figure that was well under 200 prior to 1999. This data reveals a global picture whereby violent non-state actors target energy infrastructures to air grievances, communicate to governments, impact state economic interests, or capture revenue in the form of hijacking, kidnapping ransoms, theft. And, for politically motivated groups, such as those engaged in insurgencies, attacking industry assets garners media coverage serving as a facilitator for international attention. This research note will introduce EIAD and position its utility within various research areas where the targeting of energy infrastructure, or more broadly energy infrastructure vulnerability, has been addressed, either directly or indirectly. We also provide a snapshot of the initial analysis of the data between 1980-2011, noting specific temporal and spatial trends, and then conclude with a brief discussion on the contribution of EIAD, highlighting future research trajectories. 

  15. Comparative study of potato cultivation through micropropagation ...

    African Journals Online (AJOL)

    A trial was carried out to evaluate the productivity of Solanum tuberosum L. cultivated through conventional farming and micropropagation method. Survival rate, biomass and tuber yield of both micropropagated and tuber propagated potatoes was evaluated. Survival percentages of potatoes were 90% for conventional ...

  16. Genetic fidelity in micropropagated plantlets of Ochreinauclea ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-06

    Jul 6, 2009 ... show the genetic similarity among the 22 plants (21 micropropagated plants and 1 donor mother plant). In the present study, ..... Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using. RAPD markers. Plant Cell Rep. 18: 193-197. Guo WL, Gong L, Ding ZF, ...

  17. Bite-size research: BMC Research Notes goes back to its roots.

    Science.gov (United States)

    Krüger, Dirk; Marshall, Diana M

    2017-02-14

    Since it first launched in 2008, BMC Research Notes has been a place where researchers can publish short notes and observations, research outputs which are useful for the community but which can end up hidden in the lab notebook or as a footnote in a dataset. In order to re-affirm the importance of publishing these kinds of outputs, the journal is renewing its focus on publishing note articles as well as other potentially dark data such as short null results. Publishing these articles is useful for many researchers, therefore we are also expanding the scope to all scientific and clinical disciplines including health sciences, life sciences, physical sciences, mathematics and all engineering disciplines. With this refocusing of BMC Research Notes back to its original vision, BioMed Central is offering a home for short communications to make dark data and single observations widely available to the global research community.

  18. Micropropagation of Jujube (Ziziphus jujuba

    Directory of Open Access Journals (Sweden)

    A. Khazaei

    2016-02-01

    Full Text Available Introduction: Jujube (Ziziphus jujuba is one of the most important fruit trees in Asia which has been planted from 3,000 years ago in China for medicinal purposes. Jujube belongs to the Rhamnaceae family. The Jujube fruit is used in fresh and dry forms. The fruit is full of vitamin C and has anticancer and medicinal effects. This tree can grow on salty and dry lands in Iran. Therefore, increasing the cultivation area of Jujube can be effective for soil conservation. In the last 20years, cultivation of Jujube is is considerable in Iran specially in the South Khorasan Province and 98 % of total production of Jujube in Iran belongs to this province. The low rate of seed germination and low production of shootlets are the most important problems in Jujube proliferation, so micropropagation of this plant through tissue culture was considered. Materials and methods: In this study, Cangan ecotype of Jujube was used for multiple shoot regeneration. At the end of May, apical buds of shoots were cut from mature trees of the Research Collection of Jujube at Sarbishe, Birjand, South Khorasan Province in Iran. The buds were disinfected with 70% ethanol for 1 min and 2% sodium hypoclorite for 25 min. Then the buds were rinsed with distilled water for 25 min completely. Apical buds were placed on Murashige and Skoog (MS medium supplemented with different concentrations of BA (0.5, 1, 1.5, 2 mg/L in combination with IBA or NAA (0, 0.1, 0.2, 0.4 mg/L. After one month, the shoots with 3-5 cm length were transferred to rooting media (1/2 MS + IBA or IAA : 0.5, 2, 5, 10 mg/L. The data were recorded after shooting and rooting and were analysed in the facorial experiment. Results and Discussion: The results of variance analysis and mean comparisons showed that there are differences between different levels of IBA and BA alone for the number of shoots and their length (P

  19. Acclimatization of Phalaenopsis and Cattleya obtained by micropropagation

    OpenAIRE

    Lucía Primitiva Díaz; Jorge Julio Namur; Sebastián Agustín Bollati; Osvaldo Ernesto Antonio Arce

    2010-01-01

    Título en español: Aclimatización de Phalaenopsis y Cattleya obtenidas por micropropagación ABSTRACT The quality of micropropagated plants relies on the acclimatization stage. This research intends to develop an efficient protocol to obtain the acclimatization of Phalaenopsis and Cattleya. Plants of Phalaenopsis obtained from protocorms were selected. They came from flowering stalks grown at modified Murashige and Skoog (MS) (1962) medium and classified by growth ranks and put into mo...

  20. [The archeology of slavery on Jesuit fazendas: first research notes].

    Science.gov (United States)

    Symanski, Luís Cláudio P; Gomes, Flávio

    2012-12-01

    These preliminary research notes present theoretical and methodological questions regarding a recently inaugurated investigation in historical archeology that intends to analyze daily life under slavery, demographic regimes, cultural practices, and so on. A survey of archeological sites on former 'senzalas' (slave quarters) and slave-owning fazendas in the Paraíba Valley and northern part of the state of Rio de Janeiro is currently in progress. With the cooperation of historians, archeologists, and anthropologists, records of the material culture of slave populations, which originally comprised indigenes and later Africans, are being located at excavations underway on the fazenda that is part of the Jesuit school in Campos dos Goytacazes, Rio de Janeiro, first run by the clergy and later by members of the laity in the seventeenth, eighteenth, and nineteenth centuries.

  1. To inhabit research-creation labs notes from the experience

    Directory of Open Access Journals (Sweden)

    Mónica Marcell Romero Sánchez

    2012-06-01

    Full Text Available Current arts practices have exceeded their limits to the point that a creator is considered to be a deep thinker of her/himself and of the actions that she/ he accomplishes in specific contexts. Therefore, arts practices are formed by the tradition of their disciplines and their numerous transformations, as well as by a high reflexive component around its social function of integrating the community. Based on such a premise, I will deal with some considerations about the national program of visual arts education in the informal field: the research-creation labs (implemented in 2004 under the tutelage of the Ministry of Culture – Arts Department that have influenced the way of teaching arts in our country and their ways of passing around and articulating with the Regional and National Artists Rooms. The shared reflections are part of the research project: ‘Among institutional discourses and micronarratives. Notes for the construction of a critical look at the visual arts research-creation labs’, which is in process and has its interest focused on discussing the arts practices with respect to the pedagogical practices in the institutional framework they are part of and on demonstrating in this way their tensions.

  2. Micropropagation of pear (Pyrus sp.).

    Science.gov (United States)

    Reed, Barbara M; Denoma, Jeanine; Wada, Sugae; Postman, Joseph

    2013-01-01

    Elements of micropropagation include establishment of shoot tip cultures, proliferation, rooting, and acclimatization of the resulting plantlets. The wide genetic variation in Pyrus makes micropropagation challenging for many genotypes. Initiation of shoots is most successful from forced dormant shoots or from scions grafted onto seedling rootstocks to impose juvenility. Clean shoots are recovered after testing for contaminants at the initiation stage on ½ strength Murashige and Skoog 1962 medium (MS), at pH 6.9 for 1 week or by streaking on nutrient agar. Although pear species and cultivars are cultured on several well-known media, MS is the most commonly used. Our studies showed that multiplication and growth of shoots are best on Pear Medium with higher concentrations of calcium chloride, potassium phosphate, and magnesium sulfate than MS medium and 4.4 μM N(6) benzyladenine. Pear shoots are often recalcitrant to rooting; however, a 5 s dip in 10 mM indole-3-butyric acid or naphthalene acetic acid before planting on basal medium without plant growth regulators is effective for many genotypes. Pear shoots store well at 1-4°C, and can hold for as long as 4 years without reculture. Cryopreservation protocols are available for long-term storage of pear shoot tips. Acclimation of in vitro-rooted or micrografted shoots in a mist bed follows standard procedures.

  3. Micropropagation of Plectranthus edulis (Vatke) Agnew from ...

    African Journals Online (AJOL)

    SAM

    2014-09-03

    Sep 3, 2014 ... Key words: In vitro propagation, meristem culture, micropropagation, Plectranthus edulis (Vatke) Agnew, shoots multiplication. ... P. edulis showed the occurrence of diseases (Taye,. 2008). ..... Asian J. Plant Sci. 2:616-622.

  4. Detection of somaclonal variation in micropropagated Hibiscus ...

    African Journals Online (AJOL)

    User

    Paper Accepted on 26 July 2010. Abstract. The main ... micropropagated propagules of ornamental pineapple (Santos et al., 2008) and in cucumber ... fleshy calyces are used for wine making, juice, jam, jelly, syrup, gelatin, pudding, ice cream ...

  5. Handicaps for the large scale commercial application of micropropagation.

    NARCIS (Netherlands)

    Pierik, R.L.M.

    1988-01-01

    In the last 10 years micropropagation has shown a spectacular development. However, at present the widespread use of micropropagation is handicapped by the following facts: Frequently mutations occur, particularly when applying the adventitious bud technique and callus systems. Basic knowledge

  6. Micropropagation of PLUCHEA LANCEOLATA (Oliver

    Directory of Open Access Journals (Sweden)

    M. Kher Mafatlal

    2014-09-01

    Full Text Available Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962 - MS medium supple–mented with 6-benzylaminopurine (BAP, kinetin (Kin, thidiazuron (TDZ and 2iP (2-isopentenyladenine at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3. The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ. In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

  7. A Guide to Field Notes for Qualitative Research: Context and Conversation.

    Science.gov (United States)

    Phillippi, Julia; Lauderdale, Jana

    2018-02-01

    Field notes are widely recommended in qualitative research as a means of documenting needed contextual information. With growing use of data sharing, secondary analysis, and metasynthesis, field notes ensure rich context persists beyond the original research team. However, while widely regarded as essential, there is not a guide to field note collection within the literature to guide researchers. Using the qualitative literature and previous research experience, we provide a concise guide to collection, incorporation, and dissemination of field notes. We provide a description of field note content for contextualization of an entire study as well as individual interviews and focus groups. In addition, we provide two "sketch note" guides, one for study context and one for individual interviews or focus groups for use in the field. Our guides are congruent with many qualitative and mixed methodologies and ensure contextual information is collected, stored, and disseminated as an essential component of ethical, rigorous qualitative research.

  8. Epigenetic changes detected in micropropagated hop plants.

    Science.gov (United States)

    Peredo, Elena L; Arroyo-García, Rosa; Revilla, M Angeles

    2009-07-01

    Micropropagation is a widely used technique in hops (Humulus lupulus L.). However, to the best of our knowledge, the genetic and epigenetic stability of the microplants has never been tested before. In the present study, two hop accessions were established in vitro and micropropagated for 2 years. The genetic and epigenetic stability of the in vitro plants was analyzed with several molecular techniques: random amplified DNA polymorphism (RAPD), retrotransposon microsatellite amplified polymorphism (REMAP), and methylation-sensitive amplification polymorphism (MSAP). No genetic variation among control and treated plants was found, even after 12 cycles of micropropagation. Epigenetic variation was detected, first, when field and in vitro samples were compared. Nearly a 30% of the detected fragments presented the same pattern of alterations in all the vitroplants. Second, lower levels of epigenetic variation were detected among plants from the different subcultures. Part of this detected variation seemed to be accumulated along the 12 sequential subcultures tested.

  9. Call for concept notes: Research on Myanmar's decentralization and ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    ... are launching this research call under a new initiative called Knowledge for ... Over the decades of military rule, information sharing, research, and freedom of ... Research outputs would take the form of working papers for peer-review and ...

  10. Notes to the Research on Political Socialisation in Hungary.

    Science.gov (United States)

    Keri, L.

    1982-01-01

    Hungarian research on political socialization is reviewed. The research is analyzed in terms of how the concept of political socialization is interpreted, the problems of interpreting political socialization, and the problems of judging the political socialization of youth. (AM)

  11. Notes toward a Philosophy of Action Learning Research

    Science.gov (United States)

    Coghlan, David; Coughlan, Paul

    2010-01-01

    The philosophical foundations of action learning research have not received a great deal of attention. In the context of action learning postgraduate and professional programmes in universities, articulation of a philosophy of action learning research seems timely and appropriate. This article explores a philosophy of action learning research,…

  12. Editor's Note Responding to suggestions from the research ...

    African Journals Online (AJOL)

    Admin

    Responding to suggestions from the research fellowship of the Institute of African Studies for a re-branding of the. Research Review, which began publication in the early 1960s soon after the establishment of the Institute, the old title has now been replaced with a new title — Contemporary Journal of African Studies. This is ...

  13. A Research Note on American Indian Criminal Justice

    Science.gov (United States)

    Braunstein, Rich; Anderson, Bill

    2008-01-01

    One confronts many difficulties when conducting policy-relevant criminal justice research that focuses on American Indian interests. Foremost among these difficulties is the great variation in relevant contexts that apply to this area of research. From the urban context of large American cities, where American Indians constitute a slim minority…

  14. Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from ...

    African Journals Online (AJOL)

    Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants. Evelyn Duarte, Pedro Sansberro, Claudia Luna. Abstract. Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with ...

  15. Detection of somaclonal variation in micropropagated Hibiscus ...

    African Journals Online (AJOL)

    The main objective of micropropagation is to produce clones i.e. plants which are phenotypically and genetically identical to the mother plants. The culture of organized meristems usually guarantees the production of true-to-type plants but variations in the progenies have been widely reported. Hibiscus sabdariffa L. plants ...

  16. Comparative study of potato cultivation through micropropagation ...

    African Journals Online (AJOL)

    sonu

    Comparative study of potato cultivation through micropropagation and conventional farming methods .... and Murate potash were used as fertilizer source of nitrogen, phosphorus and potassium, respectively (Table 1) ..... through Tissue culture- Application and Feasibility. U.S.D.A.,. Beltsville. Agric. Res. Sci. Educ. Admin.

  17. Research note : field control of asphalt concrete paving mixtures.

    Science.gov (United States)

    1995-01-01

    The goal of this study was to develop information and evaluate new methods for controlling quality of the AC mixture in the mat. Specifically, this research project evaluated a gyratory compactor in the field laboratory to determine mix quality. Spec...

  18. Research Note: Headteacher Support Groups Initiative within the ...

    African Journals Online (AJOL)

    Eastern Africa Social Science Research Review. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 18, No 1 (2002) >. Log in or Register to get access to full text downloads.

  19. Sibling relationships of anxiety disordered children--a research note

    NARCIS (Netherlands)

    Lindhout, Ingeborg E.; Boer, Frits; Markus, Monica T.; Hoogendijk, Thea H. G.; Maingay, Ragna; Borst, Sophie R.

    2003-01-01

    There is a paucity of knowledge on the role of sibling relationships in internalizing disorders. Research in nonclinical populations suggests an association between internalizing problems in children and negative sibling interactions. Further, an association is reported between internalizing

  20. Research notes : durability of composite repairs on bridges.

    Science.gov (United States)

    2009-08-01

    The research showed that conditions that allow moisture to get under the carbon fiber reinforced polymer composites (CFRP) combined with freeze-thaw were detrimental to durability. In addition, the results showed that the American Concrete Institute ...

  1. Inclusive Education's Promises and Trajectories: Critical Notes about Future Research on a Venerable Idea

    Science.gov (United States)

    Artiles, Alfredo J.; Kozleski, Elizabeth B.

    2016-01-01

    The purpose of this article is to offer critical notes on inclusive education research in the U.S. We discuss issues germane to conceptual clarity and the ways in which inclusive education interacts with reforms that share equity goals, noting disruptions and unintended consequences that arise at the nexus of these reforms. In addition, we…

  2. Research note: Extracting a Cycvle from Ethiopian Agricultural ...

    African Journals Online (AJOL)

    This study has two objectives. First, to extract a cycle from agricultural GDP. Second, to compute the periodicity of the cycle. To achieve these objectives, a univariate time series procedure is applied. It is found that agricultural GDP is cyclic and that the cycle occurs every 8.25 years. (Eastern Africa Social Science Research ...

  3. RESEARCH NOTE THE PERFOR]\\IANCE DURING WINTER, OF ...

    African Journals Online (AJOL)

    THE PERFOR]\\IANCE DURING WINTER, OF HETFERS FED GRASS SILAGE, MADE UNDER. UNFAVOURABLE WEATHER CONDITIONS AND E. curvula HAY, PRODUCED. FROM THE SAME SWARD. Receipt of MS: 06-10-1981. A. van Niekerk. Cedara Agriculrural Research Station, PlBag X9059, Pietermaritzburg ...

  4. RESEARCH NOTE POST-WEANING BODY MASS GAIN OF FOUR ...

    African Journals Online (AJOL)

    POST-WEANING BODY MASS GAIN OF FOUR BREEDS OF STEERS ON DIFFERENT. NUTRITIONAL LEVELS. H.P. Eloff and C.J.F. Liidemann. Mara Research Station, Msa 0922. Receipt of MS. 4.8.1977. In South Africa there are 2O breeds of cattle which are mainly kept for the purpose of beef production (van. Marle ...

  5. Managing Conflict in Teams and Examining Hiring Assumptions. Research Notes.

    Science.gov (United States)

    Jordan, Debra J.

    1996-01-01

    Research shows that well-managed conflicts can enrich a group, if good (cognitive) conflict is encouraged and bad (affective) conflict is discouraged. A model developed to understand how disabled people are treated at work suggests that there is a need to change beliefs about, as well as behavior towards, disabled people. Implications for camp are…

  6. Liberalism: Notes on a Concept for Educators and Educational Researchers

    Science.gov (United States)

    Cooley, Aaron

    2009-01-01

    This paper investigates the history and concept of liberalism from its first uses to its most modern incarnations. The paper aims to serve as a primer for educators and educational researchers to better understand liberalism and its place as a foundational and controversial discourse in politics, social science, and education. Through reference to…

  7. Amusement Arcades Help Identify Teen Needs. Research Notes.

    Science.gov (United States)

    Jordan, Debra J.

    1995-01-01

    Reviews research on youth motivation for visiting amusement arcades and on the relationship among the school achievement, socioeconomic status, and self-esteem of fourth graders. Implications for camp involve providing adolescents with unstructured leisure time with little overt adult supervision and providing early intervention for low-achieving…

  8. Micropropagation of Alibertia edulis Rich

    Directory of Open Access Journals (Sweden)

    Fernanda Augusta Boato da Silva

    2008-12-01

    Full Text Available The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA alone or combined with indole-3-butyric acid (IBA was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures also had a positive effect on both sprouting and shoot elongation. Activated charcoal was essential for rooting, and all rooted plants were able to acclimatize.Foi desenvolvido um protocolo de micropropagação para A. edulis, uma espécie arbórea frutífera e medicinal presente no Cerrado brasileiro. Sementes de A. edulis foram esterilizadas e germinadas em ágar-água. Explantes de 15 mm foram retirados das plântulas e cultivados por 50 dias em meio de multiplicação e três subculturas. A citocinina benzilalanina (BA, sozinha ou em combinação com ácido indol butírico (IBA foram adicionados em no meio ¼ MS. Os tratamentos contendo combinações de BA-IBA induziram uma maior proliferação de brotos que aqueles somente com BA, e a concentração ótima desses reguladores de crescimento foi de 0.5 mg.L-1 para cada. Os brotos de A. edulis facilmente se alongaram e a presença de reguladores de crescimento no meio de cultura teve efeito positivo sobre o alongamento. Da mesma forma, as consecutivas subculturas tiveram um efeito positivo tanto sobre a brotação quanto no alongamento. Carvão ativado foi essencial para o enraizamento dos ramos, e todas as plantas foram aclimatadas com sucesso.

  9. Multi-level barriers to LTBI treatment: a research note.

    Science.gov (United States)

    Hill, Linda; Blumberg, Elaine; Sipan, Carol; Schmitz, Katharine; West, Joshua; Kelley, Norma; Hovell, Melbourne

    2010-08-01

    This study describes the barriers to effective and timely LTBI treatment encountered in a research study on INH adherence in Latino adolescents. Participant study logs were reviewed, results of continuing medical education pretests for medical providers were examined, and participating medical facilities were contacted in order to construct a profile of multi-level barriers to LTBI treatment. A total of 285 TST positive Latino (96%) high school students were recruited into the trial. We encountered a lack of understanding of the gravity of tuberculosis infection among both the public and providers of health care. Parents and adolescents cited competing priorities, transportation problems and financial constraints as reasons for non-compliance. Improved education of the public and physicians is needed regarding the gravity of the disease and the value of treatment, as well as public and financial support for LTBI treatment by both the government and the medical community.

  10. Service quality in alcohol treatment: a research note.

    Science.gov (United States)

    Resnick, Sheilagh M; Griffiths, Mark D

    2011-01-01

    The purpose of this paper is to evaluate service quality in a UK privately funded alcohol treatment clinic. Data were gathered via interviews with two groups of participants using the SERVQUAL questionnaire. The first group comprised 32 patients and the second 15 clinic staff. The SERVQUAL instrument measures service quality expectations and perceptions across five service dimensions and identifies gaps between service expectations and perceptions of what was delivered. Patients' service quality expectations were exceeded on four of five dimensions. However, staff members felt services fell below expectations on four of five dimensions with the "reliability" service dimension emerging as the common service element falling below expectations for both participant groups. It was concluded that achieving consistent service delivery and increasing empathy between staff and patients improves overall service quality perceptions. The paper relies on self-report methods from a relatively small number of individuals. There have been limited research studies measuring alcohol treatment service quality in the private sector.

  11. Research Notes ~ Selecting Research Areas and Research Design Approaches in Distance Education: Process Issues

    Directory of Open Access Journals (Sweden)

    Sudarshan Mishra

    2004-11-01

    Full Text Available The purpose of this paper is to study the process used for selecting research areas and methodological approaches in distance education in India. Experts from the field of distance education in India were interviewed at length, with the aim of collecting qualitative data on opinions on process-issues for selecting areas for research, research design, and appropriate methodological approaches in distance education. Data collected from these interviews were subjected to content analysis; triangulation and peer consultation techniques were used for cross-checking and data verification. While the findings and recommendations of this study have limited application in that they can only be used in the specific context outlined in this paper, respondents in this study nonetheless revealed the pressing need for more process-oriented research in examining media and technology, learners and learning, and distance learning evaluation processes. Our research, which yielded interesting empirical findings, also determined that a mixed approach – one that involves both quantitative and qualitative methods – is more appropriate for conducting research in distance education in India. Qualitative evidence from our research also indicates that respondents interviewed felt that emphasis should be placed on interdisciplinary and systemic research, over that of traditional disciplinary research. Research methods such as student self-reporting, extensive and highly targeted interviews, conversation and discourse analysis, were determined to as useful for data collection for this study.

  12. Research note

    DEFF Research Database (Denmark)

    Cilliers, S C; Hayes, J P; Sales, J

    1998-01-01

    Apparent and true metabolisable energy (ME) values, corrected for zero nitrogen retention, of lucerne and barley were compared in balance studies between young (six months old, 50 to 60 kg BW) and mature (30 months old, 110 to 120 kg BW) ostriches. Birds were housed in individual metabolism crate...

  13. RESEARCH NOTE

    Indian Academy of Sciences (India)

    decade of life, secondary to renal and cardiovascular complications or stroke. ... Genomic DNA was extracted from 10 mL of peripheral venous blood, collected in ... ischemia, cerebrovascular accidents, hypertension, dysrhythmias, and renal ...

  14. Micropropagation of African violet (Saintpaulia ionantha Wendl.).

    Science.gov (United States)

    Shukla, Mukund; Sullivan, J Alan; Jain, Shri Mohan; Murch, Susan J; Saxena, Praveen K

    2013-01-01

    Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of explants used, physiological state of the mother plant, plant growth regulators in the culture medium, and growth conditions. Development of cost-effective protocols with a high rate of multiplication is a crucial requirement for commercial application of micropropagation. The current chapter describes an optimized protocol for micropropagation of African violets using leaf explants obtained from in vitro grown plants. In this process, plant regeneration occurs via both somatic embryogenesis and shoot organogenesis simultaneously in the explants induced with the growth regulator thidiazuron (TDZ; N-phenyl-N'-1,2,3-thidiazol-5-ylurea). The protocol is simple, rapid, and efficient for large-scale propagation of African violet and the dual routes of regeneration allow for multiple applications of the technology from simple clonal propagation to induction or selection of variants to the production of synthetic seeds.

  15. Endophytes in commercial micropropagation - friend or foe?

    Directory of Open Access Journals (Sweden)

    Rödel, Philipp

    2016-07-01

    Full Text Available Medicinal and aromatic plants are superorganisms like all plant species- naturally colonized by bacteria, fungi and protists. Micropropagated plants are facing different challenges under in vitro and ex vitro conditions: Mixotrophic growth under low light conditions on artificial nutrient media, poor gas exchange in small vessels, abiotic stress, bad rooting, transplanting stress, low survival rate during acclimatization in greenhouse. The use of endophytes in micropropagation can improve plant growth, yield, and health and induce tolerance to abiotic and biotic stress. A tool for the use of competent endophytes in micropropagation under in vitro and ex vitro conditions is “biotization” of plantlets with useful bacterial and fungal inocula. Fungal inocula which are used commercially are e.g. arbuscular mycorrhizal fungi in form of spores and extraradical mycelium on different carrier materials like expanded clay, vermiculite, sand or peat. Furthermore representatives of the root fungal genus Trichoderma are applied as spores formulated in powder. Plantgrowth promoting rhizobacteria of the important genera Bacillus, Pseudomonas, Azospirillum and Azotobacter in form of lyophilised endospores/bacterial cells in powder or liquid formulation are also available on the market.

  16. Quality of outpatient clinical notes: a stakeholder definition derived through qualitative research

    Directory of Open Access Journals (Sweden)

    Hanson Janice L

    2012-11-01

    content. Conclusions Perspectives of these four stakeholder groups provide a comprehensive description of quality in outpatient clinical documentation. The resulting description of characteristics and content necessary for quality notes provides a research-based foundation for assessing the quality of clinical documentation in outpatient health care settings.

  17. Quality of outpatient clinical notes: a stakeholder definition derived through qualitative research.

    Science.gov (United States)

    Hanson, Janice L; Stephens, Mark B; Pangaro, Louis N; Gimbel, Ronald W

    2012-11-19

    groups provide a comprehensive description of quality in outpatient clinical documentation. The resulting description of characteristics and content necessary for quality notes provides a research-based foundation for assessing the quality of clinical documentation in outpatient health care settings.

  18. Micropropagation of important bamboos: A review | Mudoi | African ...

    African Journals Online (AJOL)

    ... tissue culture mediated biotechnological interventions made in bamboo for large scale micropropagation, that being the need of the hour. Keywords: Bamboo, micropropagation, somatic embryogenesis, in vitro flowering, macroproliferation, field performance, clonal fidelity. African Journal of Biotechnology Vol. 12(20), pp.

  19. Micropropagation of bulbous crops: technology and present state

    NARCIS (Netherlands)

    Klerk, de G.J.M.

    2012-01-01

    Conventional propagation of bulbous crops must be supplemented with micropropagation to satisfy the requirements of present-day horticulture with respect to fast production of disease-free, superior starting material. Adequate micropropagation protocols for bulbous crops are therefore a sine qua

  20. Plantform Bioreactor for Mass Micropropagation of Date Palm.

    Science.gov (United States)

    Almusawi, Abdulminam H A; Sayegh, Abdullah J; Alshanaw, Ansam M S; Griffis, John L

    2017-01-01

    A novel protocol for the commercial production of date palm through micropropagation is presented. This protocol includes the use of a semisolid medium alternation or in combination with a temporary immersion system (TIS, Plantform bioreactor) in date palm micropropagation. The use of the Plantform bioreactor for date palm results in an improved multiplication rate, reduced micropropagation time, and improved weaning success. It also reduces the cost of saleable units and thus improves economic return for commercial micropropagation. The use of the Plantform bioreactor successfully addresses other hindrances that can occur during the scale-up of date palm micropropagation, including asynchrony of somatic embryos, limited maturation of somatic embryos, and highly variable germination frequencies of embryos.

  1. Micropropagation of Wild Service Tree (Sorbus torminalis [L.] Crantz): The Regulative Role of Different Aromatic Cytokinins During Organogenesis

    Czech Academy of Sciences Publication Activity Database

    Malá, J.; Máchová, P.; Cvrčková, H.; Karady, Michal; Novák, Ondřej; Mikulík, Jaromír; Hauserová, Eva; Greplová, Jarmila; Strnad, Miroslav; Doležal, Karel

    2009-01-01

    Roč. 28, č. 4 (2009), s. 341-348 ISSN 0721-7595 R&D Projects: GA ČR GA206/07/0570; GA MŠk 1M06030 Institutional research plan: CEZ:AV0Z50380511 Keywords : Wild service tree * Micropropagation * Rooting Subject RIV: EF - Botanics Impact factor: 2.438, year: 2009

  2. Advancing medicine one research note at a time: the educational value in clinical case reports

    Directory of Open Access Journals (Sweden)

    Cabán-Martinez Alberto J

    2012-07-01

    Full Text Available Abstract A case report—a brief written note that describes unique aspects of a clinical case—provides a significant function in medicine given its rapid, succinct, and educational contributions to scientific literature and clinical practice. Despite the growth of, and emphasis on, randomized clinical trials and evidenced-based medicine, case reports continue to provide novel and exceptional knowledge in medical education. The journal BMC Research Notes introduces a new “case reports” section to provide the busy clinician with a forum in which to document any authentic clinical case that provide educational value to current clinical practice. The aim is for this article type to be reviewed, wherever possible, by specialized Associate Editors for the journal, in order to provide rapid but thorough decision making. New ideas often garnered by and documented in case reports will support the advancement of medical science — one research note at a time.

  3. Advancing medicine one research note at a time: the educational value in clinical case reports.

    Science.gov (United States)

    Cabán-Martinez, Alberto J; Beltrán, Wilfredo F García

    2012-07-06

    A case report--a brief written note that describes unique aspects of a clinical case--provides a significant function in medicine given its rapid, succinct, and educational contributions to scientific literature and clinical practice. Despite the growth of, and emphasis on, randomized clinical trials and evidenced-based medicine, case reports continue to provide novel and exceptional knowledge in medical education. The journal BMC Research Notes introduces a new "case reports" section to provide the busy clinician with a forum in which to document any authentic clinical case that provide educational value to current clinical practice. The aim is for this article type to be reviewed, wherever possible, by specialized Associate Editors for the journal, in order to provide rapid but thorough decision making. New ideas often garnered by and documented in case reports will support the advancement of medical science--one research note at a time.

  4. Micropropagation of eucalyptus saligna sm. from cotyledonary nodes

    International Nuclear Information System (INIS)

    Silva, A.L.L.D.; Brondani, G.E.; Horbach, A.

    2015-01-01

    Eucalyptus saligna is an important woody plant used to lumber and cellulose. The aim of this research was to establish a protocol for micropropagation of this species from cotyledonary nodes. Plantlets with 16 days old were used as a donor explants. The induction of cotyledonary nodes consisted of two parts: a dark culture followed by a light culture. Basal medium was MS added with 30g.L-1 sucrose, 10% coconut water and solidified with 7g.L-1 agar. For the dark culture the media were supplemented with 3.6 micro M NAA (Naftalenoacetic acid) and 4.4 micro M BAP (6-Benzylaminopurine) and for the light culture the media were supplemented with 2.7 micro M NAA and 1.1 micro M BAP. The period for dark and light culture was 20 days. Shoots were multiplied on MS medium, 30 g.L-1 sucrose supplemented with 1.1 micro M BAP. Shoots were elongated on MS medium free of plant growth regulators. Shoots were rooting on half-strength MS salts. Acclimatization was performed in a hydroponics floating system. Moreover, the shoot multiplication in liquid medium with different CaCl/sub 2/ levels was carried out under agitation. Organogenesis of cotyledonary nodes was characterized by simultaneous occurrence of shoot and callus. Shoots presented hyperhydricity under liquid medium, however, the CaCl/sub 2/ reduces the hyperhydricity in liquid medium; nevertheless, it had been not effective in eliminating hyperhydricity due to toxicity of chlorine. The hydroponics acclimatization results in 90% plant survival. An efficient protocol for micropropagation of E. saligna was suitable established and can be used for clonal propagation or genetic transformation. (author)

  5. Research Note: Helping Students Market Themselves with "The Power of Who!"

    Science.gov (United States)

    Wysong, Scott; Munoz, Laura

    2017-01-01

    While learning is still of paramount importance, today, colleges and universities realize that they need to equip their students with the skills and knowledge to get hired after graduation. Using the book "The Power of Who!," written by experienced executive recruiter Bob Beaudine, this research note looks at what kind of impact the…

  6. Note-Taking Techniques for Students with Disabilities: A Systematic Review of the Research

    Science.gov (United States)

    Boyle, Joseph R.; Rivera, Tina Z.

    2012-01-01

    This article provides a synthesis of note-taking research among students with learning disabilities or other high-incidence disabilities. A search of the professional literature between 1980 and 2010 yielded nine intervention studies, which were evaluated either in terms of effect size or percentage of nonoverlapping data. The studies examined…

  7. Research Note The reliability of a field test kit for the detection and ...

    African Journals Online (AJOL)

    Research Note The reliability of a field test kit for the detection and the persistence of ... Open Access DOWNLOAD FULL TEXT ... The objectives were to test a field kit for practicality and reliability, to assess the spread of the bacteria among ...

  8. The influence of anticipated regret on business start-up behaviour: A research note

    NARCIS (Netherlands)

    Hatak, Isabella; Snellman, Kirsi

    This research note aims to develop the emotional underpinning of early-stage entrepreneurship by examining the influence of anticipated regret, a negative emotion, on the transformation of latent entrepreneurs into nascent entrepreneurs. Drawing on regret regulation theory and two waves of survey

  9. Micropropagation of Ajuga bracteosa, a medicinal herb

    OpenAIRE

    Kaul, Shivanee; Das, Sandip; Srivastava, P. S.

    2013-01-01

    For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, l...

  10. Efficient micropropagation of highly economic, medicinal and ornamental plant Lallemantia iberica (Bieb.) Fisch. and C. A. Mey.

    Science.gov (United States)

    Ozdemir, Fethi Ahmet; Yildirim, Mehmet Ugur; Pourali Kahriz, Mahsa

    2014-01-01

    Lallemantia iberica (Bieb.) Fisch. and C. A. Mey is high valued annual ornamental and medicinal plant from Lamiaceae family that prefers dry sunny hillsides, roadsides, slopes, and fallow fields over an altitude of 500-2150 m. It bears beautiful white flowers and bloom from April to June each year. This study reports L. iberica micropropagation using cotyledon node explants isolated from 15-day-old in vitro regenerated plantlets. The cotyledon node explants were cultured on MS medium containing 0.50, 1.00 plus 2.00 mg/L BAP, 0.00, 0.01, and 0.02 mg/L NAA. Maximum shoot regeneration was noted on MS medium containing 0.50 mg/L BAP. Well-developed micropropagated shoots were rooted on MS medium containing 1.00 mg/L IBA. The rooted plants were easily hardened in the growth chamber and acclimatised in greenhouse.

  11. Micropropagation of peach rootstocks and cultivars.

    Science.gov (United States)

    Balla, Ildikó; Mansvelt, Lucienne

    2013-01-01

    Peach (Prunus persica (L.) Batsch) is one of the most popular stone fruits, commercially produced largely in Mediterranean and, to a lesser extent, in continental climatic conditions. Several breeding programs with different aims release annually large numbers of new cultivars. Micropropagation offers a suitable method to provide the growers of sufficient quantities of rootstocks, as well as of pathogen-free planting material of old and new cultivars.An effective four-step micropropagation procedure for cultivar and rootstock production is described here, based on the use of modified MS and WPM media. The health status of the initial shoot tips is very important, also because the growth and proliferation rate of shoot cultures from virus-infected clones are generally very poor. Proliferation and elongation phases depend on the major macro-elements, as well as the content and ratio of plant growth regulators. It is important to grow the cultures at 22°C, as hyperhydricity may develop at higher temperatures. Although sucrose is the most common carbon source used during proliferation and rooting, for some peach cultivars and rootstocks the replacement of sucrose (10 g/L) with glucose (20 g/L) in the rooting medium improves the rooting and survival rates of plants in the acclimatization phase. The rooting rate of the rootstock "Cadaman" is improved with the chelated form of iron FeEDDHA at 150 mg/L. Rooted plants are acclimatized in greenhouse under high humidity conditions.

  12. Mentha piperita micropropagation and volatil constituents evaluation

    Directory of Open Access Journals (Sweden)

    Jorge Ignacio Zapata

    1999-01-01

    Full Text Available The use of in vitro plant tissue culture techniques for the propagation of aromatic and medicinal species, could play very promising role for the economy of small farmers, due to the high potential of industrialization of their essential oils. In this work is shown how the tissue culture technique has been used for the propagation of Mentha piperita, as its essential oil is widely used in the flavor and fragrance industry. Mints from different sources were studied, and evaluation of the yield and quality of the essential oil was carry out, before and after the micropropagation by plant tissue culture. Several basal mediums with different proportion of plant growth regulators and vitamins were tested in order to maximize the propagation results. The constituents of the essential oil were quantified by Gas Chromatography. Lin & Staba (1962 basal medium, supplemented with BAP, and some vitamins, was the best for mints species micropropagation. The yield and quality of essential oil produced by plant tissue culture and its mother plant were very similar.

  13. Micropropagation and genetic transformation of Tylophora indica (Burm. f.) Merr.: a review.

    Science.gov (United States)

    Teixeira da Silva, Jaime A; Jha, Sumita

    2016-11-01

    This review provides an in-depth and comprehensive overview of the in vitro culture of Tylophora species, which have medicinal properties. Tylophora indica (Burm. f.) Merr. is a climbing perennial vine with medicinal properties. The tissue culture and genetic transformation of T. indica, which has been extensively studied, is reviewed. Micropropagation using nodal explants has been reported in 25 % of all publications. Leaf explants from field-grown plants has been the explant of choice of independent research groups, which reported direct and callus-mediated organogenesis as well as callus-mediated somatic embryogenesis. Protoplast-mediated regeneration and callus-mediated shoot organogenesis has also been reported from stem explants, and to a lesser degree from root explants of micropropagated plants in vitro. Recent studies that used HPLC confirmed the potential of micropropagated plants to synthesize the major T. indica alkaloid tylophorine prior to and after transfer to field conditions. The genetic integrity of callus-regenerated plants was confirmed by RAPD in a few reports. Tissue culture is an essential base for genetic transformation studies. Hairy roots and transgenic T. indica plants have been shown to accumulate tylophorine suggesting that in vitro biology and transgenic methods are viable ways of clonally producing valuable germplasm and mass producing compounds of commercial value. Further studies that investigate the factors affecting the biosynthesis of Tylophora alkaloids and other secondary metabolites need to be conducted using non-transformed as well as transformed cell and organ cultures.

  14. Researches on Mathematical Relationship of Five Elements of Containing Notes and Fibonacci Sequence Modulo 5

    Directory of Open Access Journals (Sweden)

    Zhaoxue Chen

    2015-01-01

    Full Text Available Considering the five periods and six qi’s theory in TCM almost shares a common basis of stem-branch system with the five elements of containing notes, studying the principle or mathematical structure behind the five elements of containing notes can surely bring a novel view for the five periods and six qi’s researches. By analyzing typical mathematical rules included in He tu, Luo shu, and stem-branch theory in TCM as well as the Fibonacci sequence especially widely existent in the biological world, novel researches are performed on mathematical relationship between the five elements of containing notes and the Fibonacci sequence modulo 5. Enlightened by elementary Yin or Yang number grouping principle of He tu, Luo shu, the 12534 and 31542 key number series of Fibonacci sequence modulo 5 are obtained. And three new arrangements about the five elements of containing notes are then introduced, which have shown close relationship with the two obtained key subsequences of the Fibonacci sequence modulo 5. The novel discovery is quite helpful to recover the scientific secret of the five periods and six qi’s theory in TCM as well as that of whole traditional Chinese culture system, but more data is needed to elucidate the TCM theory further.

  15. Micropropagation of some Malaysian banana and plantain (Musa sp ...

    African Journals Online (AJOL)

    USER

    2010-04-19

    Apr 19, 2010 ... As one of the origins of bananas, Malaysia has a great variety of them, that is, ... reduces contamination rate during micropropagation as compared to soil ..... 42. Faostat (2005). Food and Agricultural Organization of the United.

  16. Micropropagation of Eucalyptus grandis and nitens using tissue culture techniques

    Energy Technology Data Exchange (ETDEWEB)

    Furze, M.J.; Creswell, C.F.

    1985-01-01

    Experiments with nodal explants of E. grandis and E. nitens seedlings and E. grandis coppice shoots showed that a large number of plants can be produced from a single explant using micropropagation. The percentage of micropropagated shoots that formed roots was about 90% for E. grandis and 80% for E. nitens. For both species, about 90% of the rooted shoots survived after hardening off. 9 references.

  17. Micropropagation of Ajuga bracteosa, a medicinal herb.

    Science.gov (United States)

    Kaul, Shivanee; Das, Sandip; Srivastava, P S

    2013-04-01

    For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, leaf displayed quickest response followed by petiole while root was the slowest. Hardening of plantlets was achieved with 82 % survival. The hardened plants were maintained in pots with garden soil under controlled (Temp. 25 ± 2 °C) conditions. RAPD exhibited genetic fidelity with 100 % monomorphism in regenerants.

  18. Temporary Immersion System for Date Palm Micropropagation.

    Science.gov (United States)

    Othmani, Ahmed; Bayoudh, Chokri; Sellemi, Amel; Drira, Noureddine

    2017-01-01

    The temporary immersion system (TIS) is being used with tremendous success for automation of micropropagation of many plant species. TIS usually consists of a culture vessel comprising two compartments, an upper one with the plant material and a lower one with the liquid culture medium and an automated air pump. The latter enables contact between all parts of the explants and the liquid medium by setting overpressure to the lower part of the container. These systems are providing the most satisfactory conditions for date palm regeneration via shoot organogenesis and allow a significant increase of multiplication rate (5.5-fold in comparison with that regenerated on agar-solidified medium) and plant material quality, thereby reducing production cost.

  19. In the middle of the world, aquendar the methodology: notes for queering curriculum research

    Directory of Open Access Journals (Sweden)

    Thiago Ranniery Moreira de Oliveira

    2016-05-01

    Full Text Available This paper investigates how the condition of queer critique not only reflects in the sphere of social exchange, but it can acquire methodological dimensions to the extent that allows reapply to the investigative positions and the paths of curriculum research ironic and jocular style redefinition of the queer insult. The explored argument is that the queer critique allows to question the normative naturalization of research procedures into the curriculum as those contained in the formulas that tend to oppose researcher/researched, realism/fiction, coherence/multiplicity. From the perspective of taking queer as a heterogeneous reading, this text explores how four notes, combinations, wanderings, affectations and fertilizations, translate what happens when queering research methodology. Movements that can be seen as wandering experiments in relation to the paradigm of queer critique in Education and curriculum.

  20. A call for BMC Research Notes contributions promoting best practice in data standardization, sharing and publication.

    Science.gov (United States)

    Hrynaszkiewicz, Iain

    2010-09-02

    BMC Research Notes aims to ensure that data files underlying published articles are made available in standard, reusable formats, and the journal is calling for contributions from the scientific community to achieve this goal. Educational Data Notes included in this special series should describe a domain-specific data standard and provide an example data set with the article, or a link to data that are permanently hosted elsewhere. The contributions should also provide some evidence of the data standard's application and preparation guidance that could be used by others wishing to conduct similar experiments. The journal is also keen to receive contributions on broader aspects of scientific data sharing, archiving, and open data.

  1. Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench

    Science.gov (United States)

    2012-08-01

    1105 Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench Luiz H...fungal community and micropropagated clones of E. purpurea was re-established after acclimatization to soil and the endophytic fungi produced compounds...Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench 5a. CONTRACT

  2. Research Note: Yavirau: A traditional Fijian fish drive as an example of culturally embedded community development

    Directory of Open Access Journals (Sweden)

    Fink, Michael

    2012-09-01

    Full Text Available A yavirau (traditional Fijian fish drive is an ancient Fijian custom which has been adapted to today’s needs. Implemented and organised by a village community without external assistance, this highly this culturally specific custom is an example of development on a local level. According to theorists and practitioners working on development issues, such a strategy for Community Development (CD is promising because it seizes current approaches as it fosters local, decentralised, cultural specific development and aims at a high level of local participation. This research note analyses a yavirau as an example of CD, showing its advantages as well as its limitations.

  3. Field and laboratory notes on instream research - Research and Development of New Marking and Monitoring Technologies

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project addresses how to expand the current fish-tracking technologies to enable the fisheries community to successfully carry out the actions, research, and...

  4. Acclimatization and leaf anatomy of micropropagated fig plantlets

    Directory of Open Access Journals (Sweden)

    Chrystiane Fráguas Chirinéa

    2012-12-01

    Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.

  5. Micropropagation and cryopreservation of garlic (Allium sativum L.).

    Science.gov (United States)

    Keller, E R Joachim; Senula, Angelika

    2013-01-01

    Garlic (Allium sativum L.) is a very important medicinal and spice plant. It is conventionally propagated by daughter bulbs ("cloves") and bulbils from the flower head. Micropropagation is used for speeding up the vegetative propagation mainly using the advantage to produce higher numbers of healthy plants free of viruses, which have higher yield than infected material. Using primary explants from bulbs and/or bulbils (shoot tips) or unripe inflorescence bases, in vitro cultures are initiated on MS-based media containing auxins, e.g., naphthalene acetic acid, and cytokinins, e.g., 6-γ-γ-(dimethylallylaminopurine) (2iP). Rooting is accompanying leaf formation. It does not need special culture phases. The main micropropagation methods rely on growth of already formed meristems. Long-term storage of micropropagated material, cryopreservation, is well-developed to maintain germplasm. The main method is vitrification using the cryoprotectant mixture PVS3.

  6. A highly efficient protocol for micropropagation of Begonia tuberous.

    Science.gov (United States)

    Duong, Tan Nhut; Nguyen, Thanh Hai; Mai, Xuan Phan

    2010-01-01

    A protocol for micropropagation of begonia was established utilizing a thin cell layer (TCL) system. This system has been employed to produce several thousand shoots per sample. Explant size and position, and plant growth regulators (PGRs) contribute to the tissue morphogenesis. By optimizing the size of the tissue and applying an improved selection procedure, shoots were elongated in 8 weeks of culture, with an average number of 210 +/- 9.7 shoots per segment. This system has facilitated a number of studies using TCL as a model for micropropagation and will enable the large-scale production of begonia. On an average, the best treatment would allow production of about 10,000 plantlets by the micropropagation of the axillary buds of one plant with five petioles, within a period of 8 months.

  7. IMPROVEMENTS OF CLONAL MICROPROPAGATION TECHNOLOGY OF APPLE ROOTSTOCKS AT THE STAGE OF INTRODUCTION TO IN VITRO CULTURE

    OpenAIRE

    Besedina E. N.; Buntsevich L. L.

    2015-01-01

    The stage of introduction of plantlets to in vitro culture is an important stage of technology of clonal micropropagation of plants. For the purpose of decrease in a share of the plantlets that were lost from an infection and increase of their regeneration ability, sterilizers and antibiotics, effective and safe for apple rootstocks’ plantlets wee allocated, and also favorable terms of introduction to in vitro culture were allocated. As a result of the conducted researches, we have establishe...

  8. The role of topolins in micropropagation and somaclonal variation of banana cultivars ´Williams´ and ´Grand Naine´ (Musa spp. AAA)

    Czech Academy of Sciences Publication Activity Database

    Bairu, M. W.; Stirk, W.A.; Doležal, Karel; van Staden, J.

    2008-01-01

    Roč. 95, č. 3 (2008), s. 373-379 ISSN 0167-6857 R&D Projects: GA ČR GA522/06/0108 Institutional research plan: CEZ:AV0Z50380511 Keywords : Abnormality index * Banana * Micropropagation Subject RIV: ED - Physiology Impact factor: 1.017, year: 2008

  9. Micropropagation of Rubus and Ribes spp.

    Science.gov (United States)

    Dziedzic, Ewa; Jagła, Joanna

    2013-01-01

    Micropropagation is the most appropriate method for large-scale production of Rubus and Ribes spp. The proliferation rate of Rubus spp. differs in shoot tips and nodal segments. The culture media used for raspberry and blackberry propagation are MS-based supplemented with different combination and ratio of plant growth regulators, depending on the stage of culture. The initiation medium containing 0.4 mg L(-1) BA and 0.1 mg L(-1) IBA is used to stabilize shoot cultures. In multiplication media, concentration of cytokinin is doubled. In vitro rooting of shoots is achieved on media supplemented with 1.0 mg L(-1) IBA. Ribes spp. cultures are initiated from shoot tips, meristem, or dormant buds on MS medium supplemented with 2.0 mg L(-1) BA, 0.5 mg L(-1) IBA, and 0.1 mg L(-1) GA(3.) After stabilization of shoot cultures in 3-4-week time, shoot multiplication is carried out on MS medium containing 1.0 mg L(-1) BA and 0.1 mg L(-1) IBA. Shoots 2 cm long are cultured to rooting on a medium amended with 2.0 mg L(-1) IBA and 5.0 mg L(-1) IAA. Rooted plantlets are transferred to universal peat substrate and acclimatized in the greenhouse.

  10. Micropropagation of Helleborus through axillary budding.

    Science.gov (United States)

    Beruto, Margherita; Viglione, Serena; Bisignano, Alessandro

    2013-01-01

    Helleborus genus, belonging to the Ranunculaceae family, has 20 species of herbaceous perennial flowering plants. The commercial exploitation of this plant is dependent on the selection and propagation of appropriate lines. High propagation rate could be accomplished by using a suitable tissue culture method enabling the rapid introduction of valuable selections in the market. However, in vitro cultivation of Helleborus is still very difficult. Thereby the development of reliable in vitro propagation procedures is crucial for future production systems. Axillary buds cultured on agar-solidified Murashige and Skoog medium supplemented with 1 mg/L benzyladenine, 0.1 mg/L β-naphthoxyacetic acid, and 2 mg/L isopentenyl adenine develop shoots after 16 weeks of culture under 16 h light regime, 50-60 μmol/s/m(2), and 19 ± 1°C. The multiplication rate ranges from 1.4 to 2.1. However, the genotype and the number of subcultures affect the efficiency of the micropropagation process. The rooting of shoots is about 80% in solidified MS medium containing 1 mg/L 1-naphthaleneacetic acid and 3 mg/L indole-3-butyric acid. The described protocol provides information which can contribute to the commercial production of Helleborus plants.

  11. Micropropagation of the endangered shrub pondberry (Lindera melissifolia [Walt.] Blume)

    Science.gov (United States)

    Tracy S. Hawkins; Nathan M. Schiff; Emile s. Gardiner; Theodore Leininger; Margaret S. Devall; A. Dan Wilson; Paul Hamel; Deborah D. McCown; Kristina Connor

    2007-01-01

    A micropropagation protocol using shoot cultures is described for Lindera melissifolia, a federally listed endangered shrub endemic to the southeastern United States. Stock plants were harvested from native L. melissifolia populations growing in the lower Mississippi Alluvial Valley. In vitro proliferation was on woody plant medium...

  12. Associated microbial contaminants in in-vitro micropropagation of ...

    African Journals Online (AJOL)

    Studies were carried out to determine the microbial contaminants associated with in-vitro micropropagation of Ipomea batatas (sweet potato). The contaminants were found to be mostly fungal organisms, Aspergillus Spp (62%), Penicillum Spp. (31%), Fusarium Spp. (5%) and Alternaria Spp. (2%). Bacterial contamination ...

  13. Influence of various carbohydrates on the in vitro micropropagation ...

    African Journals Online (AJOL)

    Sucrose appears to be the most favorable sugar to ensure the in vitro micropropagation of N. diderrichii. Sorbose caused the necrosis of explants in culture. The mannose and the mannitol, did not cause the necrosis of explants, but their presence had an unfavorable role on roots initiation and slow down the plants' growth.

  14. Micropropagation, genetic engineering, and molecular biology of Populus

    Science.gov (United States)

    N. B. Klopfenstein; Y. W. Chun; M. -S. Kim; M. A. Ahuja; M. C. Dillon; R. C. Carman; L. G. Eskew

    1997-01-01

    Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3) molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease,...

  15. Rapid micropropagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl.

    African Journals Online (AJOL)

    Rapid micropropagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl. (a valuable medicinal plant) from shoot bud explants. NA Yusuf, MMS Annuar, N Khalid. Abstract. A successful protocol was developed for mass propagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl., an important medicinal plant. Numerous shoots ...

  16. Thin Cell Layer technology in ornamental plant micropropagation ...

    African Journals Online (AJOL)

    Thin cell layer (TCL) technology originated almost 30 years ago with the controlled development of flowers, roots, shoots and somatic embryos on tobacco pedicel longitudinal TCLs. Since then TCLs have been successfully used in the micropropagation of many ornamental plant species whose previous in vitro ...

  17. A novel approach for rapid micropropagation of maspine pineapple ...

    African Journals Online (AJOL)

    A novel approach for rapid micropropagation of maspine pineapple ( Ananas comosus L.) shoots using liquid shake culture system. ... Maspine (Ananas comosus L.) is currently the most preferred pineapple variety in Malaysia due to its pleasant aroma and applicability in caning. Large quantities of plant materials are ...

  18. Micropropagation of Guadua angustifolia Kunth (Poaceae) using a ...

    African Journals Online (AJOL)

    The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semisolid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA®), comparing results with those obtained using a ...

  19. In Vitro micropropagation of Lawsonia inermis (Lythraceae

    Directory of Open Access Journals (Sweden)

    G.R. Rout

    2001-12-01

    Full Text Available A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962 medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA, 0.25 mg/l Kinetin (Kn, 0.5 mg/l ascorbic acid and 3% (w/v sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14h photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soilSe desarrolo un protocolo exitoso para la propagación en masa de Lawsonia inermis Linn., una planta medicinal importante. Se indujeron múltiples tallos en meristamos apicales y auxiliares derivados de explantes maduros de L. inermis en medio de Murashige y Skoog (1962 suplementado con 0.25 mg/l 6-benzylaminopurina (BA, 0.25 mg/l quinetina (Kn, 0.5 mg/l ácido ascórbico y 3 % (w/v sucrosa. La tasa de multiplicación fue más alta cuando los cultivos fueron incubados bajo luz continua que bajo el fotoperíodo de 14hr. El enraizamiento se logró al tranferir los microtallos al medio basal, semi-sólido MS suplementado con 0.25 mg/l de ácido indol-3-butiríco (IBA luego de 10 días de cultivo. Las plántulas micropropagadas fueron aclimatizadas y cultivadas con éxito en el suelo

  20. Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP).

    Science.gov (United States)

    Peraza-Echeverria, S; Herrera-Valencia, V A.; Kay, A -J.

    2001-07-01

    The extent of DNA methylation polymorphisms was evaluated in micropropagated banana (Musa AAA cv. 'Grand Naine') derived from either the vegetative apex of the sucker or the floral apex of the male inflorescence using the methylation-sensitive amplification polymorphism (MSAP) technique. In all, 465 fragments, each representing a recognition site cleaved by either or both of the isoschizomers were amplified using eight combinations of primers. A total of 107 sites (23%) were found to be methylated at cytosine in the genome of micropropagated banana plants. In plants micropropagated from the male inflorescence explant 14 (3%) DNA methylation events were polymorphic, while plants micropropagated from the sucker explant produced 8 (1.7%) polymorphisms. No DNA methylation polymorphisms were detected in conventionally propagated banana plants. These results demonstrated the usefulness of MSAP to detect DNA methylation events in micropropagated banana plants and indicate that DNA methylation polymorphisms are associated with micropropagation.

  1. The Circular Economy and the Leading European Retailers: A Research Note

    Directory of Open Access Journals (Sweden)

    Peter Jones

    2018-03-01

    Full Text Available The concept of the circular economy is gaining momentum in political and business thinking about the transition to a more sustainable future. EuroCommerce and the European Retail Round Table, for example, have argued that leading retailers are keen to play a leading role in shaping the circular economy within Europe. This exploratory research note outlines the characteristic features of the concept of the circular economy, provides some illustrations of how Europe’s leading retailers are publicly addressing circular economy approaches and offers some general reflections on the application of the concept within the retail sector of the economy. The findings reveal that almost 50% of the leading European retailers signalled a commitment to the circular economy and to the principles underpinning it and a number of them looked to evidence their commitment within their retail operations. That said the authors suggest that If Europe’s leading retailers’ public commitments to a more circular economy are to become a reality then they will not only need to effect a radical change in their current business models and that this will need to be accompanied by radical changes in consumers consumption behaviour. More contentiously, there must be concerns that the leading European retailers might effectively capture the concept of the circular economy to justify continuing economic growth.

  2. Efficient micropropagation and chlorocholine chloride induced stevioside production of Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Dey, Avishek; Kundu, Sayanti; Bandyopadhyay, Abhijit; Bhattacharjee, Aloke

    2013-01-01

    A promising method of micropropagation of Stevia rebaudiana Bertoni has been developed with an aim to increase the biomass, survivability of the plantlets and stevioside production, using chlorocholine chloride (CCC). Microshoots transferred to the MS medium containing different combinations CCC and IBA were found to be most effective in terms of growth pattern, hardening ability of the plantlets and stevioside content, compared to MS medium containing either IBA or CCC. Among other combinations tested, MS medium supplemented with 3 mg/l CCC and 3 mg/l IBA was found most effective in inducing significant changes like reduced shoot length, increased number of roots, higher leaf size, increased biomass and chlorophyll retaining capacity, higher survival percentage and most importantly the elevated stevioside content. Collectively, the major observations of this research indicate that application of CCC in micropropagation of S. rebaudiana Bertoni is a promising approach and has commercial prospects. Copyright © 2012 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  3. Physiological, vascular and nanomechanical assessment of hybrid poplar leaf traits in micropropagated plants and plants propagated from root cuttings: A contribution to breeding programs.

    Science.gov (United States)

    Ďurkovič, Jaroslav; Husárová, Hana; Javoříková, Lucia; Čaňová, Ingrid; Šuleková, Miriama; Kardošová, Monika; Lukáčik, Ivan; Mamoňová, Miroslava; Lagaňa, Rastislav

    2017-09-01

    Micropropagated plants experience significant stress from rapid water loss when they are transferred from an in vitro culture to either greenhouse or field conditions. This is caused both by inefficient stomatal control of transpiration and the change to a higher light intensity and lower humidity. Understanding the physiological, vascular and biomechanical processes that allow micropropagated plants to modify their phenotype in response to environmental conditions can help to improve both field performance and plant survival. To identify changes between the hybrid poplar [Populus tremula × (Populus × canescens)] plants propagated from in vitro tissue culture and those from root cuttings, we assessed leaf performance for any differences in leaf growth, photosynthetic and vascular traits, and also nanomechanical properties of the tracheary element cell walls. The micropropagated plants showed significantly higher values for leaf area, leaf length, leaf width and leaf dry mass. The greater leaf area and leaf size dimensions resulted from the higher transpiration rate recorded for this stock type. Also, the micropropagated plants reached higher values for chlorophyll a fluorescence parameters and for the nanomechanical dissipation energy of tracheary element cell walls which may indicate a higher damping capacity within the primary xylem tissue under abiotic stress conditions. The performance of the plants propagated from root cuttings was superior for instantaneous water-use efficiency which signifies a higher acclimation capacity to stressful conditions during a severe drought particularly for this stock type. Similarities were found among the majority of the examined leaf traits for both vegetative plant origins including leaf mass per area, stomatal conductance, net photosynthetic rate, hydraulic axial conductivity, indicators of leaf midrib vascular architecture, as well as for the majority of cell wall nanomechanical traits. This research revealed that

  4. The influence of micropropagation on growth and coppicing ability of Eucalyptus polybractea.

    Science.gov (United States)

    Goodger, Jason Q D; Woodrow, Ian E

    2010-02-01

    A micropropagation protocol was recently developed for Eucalyptus polybractea R.T. Baker, a commercially important eucalypt grown in short-rotation coppice cultivation and harvested for its foliar 1,8-cineole oil. Micropropagation of elite E. polybractea trees has resulted in selection gains for foliar oil traits, but decreased above-ground biomass accumulation has been observed in clones compared to related half-sibling families. This study aims to use a greenhouse study to investigate if micropropagation induces somaclonal variation that can account for the reduction in above-ground biomass in E. polybractea clones. Secondly, the study aims to compare the coppicing ability of micropropagated clones with related half-sibling seedlings using de-topped plantation-grown saplings. The results of the greenhouse study suggest that micropropagation of E. polybractea induces somaclonal variation that manifests in more mature leaf morphologies such as increased foliar oil concentrations and lower specific leaf area (SLA), attributable to an isobilateral arrangement of increased palisade mesophyll layers. Lower SLA, rather than differences in root allocation, is likely to be a key contributor to the lower relative growth rates observed in early sapling growth of micropropagated clones. In the field study, all micropropagated and seedling-derived E. polybractea saplings coppiced vigorously in the 12 months after de-topping. The coppice growth was so vigorous in the 12 months after de-topping that total above-ground biomass equalled that of the 27-month-old saplings, irrespective of propagation source. The morphological distinction between leaves of micropropagated and seed-derived plants was no longer evident in the coppice regrowth. The results presented here suggest that the micropropagated leaf morphology and the resultant growth reduction is transient and micropropagated plants coppice just as vigorously as seed-derived plants. Therefore, micropropagation is unlikely to

  5. Micropropagation, Acclimatization, and Greenhouse Culture of Veratrum californicum.

    Science.gov (United States)

    White, Sarah A; Adelberg, Jeffrey; Naylor-Adelberg, Jacqueline; Mann, David A; Song, Ju Yeon; Sun, Youping

    2016-01-01

    Micropropagation and production of Veratrum californicum is most successful when using a premixed Murishage and Skoog basal medium with vitamins and a 5-week subculture cycle at 16 °C for multiplication. These culture conditions provide the best percent survival after acclimatization in the greenhouse. However, clone response to temperature and light quality within culture conditions varies. Micropropagated plants have mass and morphology similar to 2- or 3-year-old seedlings. Acclimatized plantlets can then be grown in the greenhouse using sub-irrigation (ebb and flood) to maintain substrate volumetric water content > 44 %. Growth cycle in the greenhouse must be about 100 days, followed by dormancy for 5 months at 5 °C.

  6. Visual object recognition for automatic micropropagation of plants

    Science.gov (United States)

    Brendel, Thorsten; Schwanke, Joerg; Jensch, Peter F.

    1994-11-01

    Micropropagation of plants is done by cutting juvenile plants and placing them into special container-boxes with nutrient-solution where the pieces can grow up and be cut again several times. To produce high amounts of biomass it is necessary to do plant micropropagation by a robotic system. In this paper we describe parts of the vision system that recognizes plants and their particular cutting points. Therefore, it is necessary to extract elements of the plants and relations between these elements (for example root, stem, leaf). Different species vary in their morphological appearance, variation is also immanent in plants of the same species. Therefore, we introduce several morphological classes of plants from that we expect same recognition methods.

  7. Successful micropropagation of the cadmium hyperaccumulator Viola baoshanensis (Violaceae).

    Science.gov (United States)

    Li, Jin-Tian; Deng, Dong-Mei; Peng, Guang-Tian; Deng, Jin-Chuan; Zhang, Jun; Liao, Bin

    2010-01-01

    Viola baoshanensis is one of the most rare cadmium (Cd) hyperaccumulators, however, it is hard to propagate. Micropropagation has been applied to solve the problems with propagation of a few heavy metal hyperaccumulators. Therefore there is a high likelihood that micropropagation may offer a suitable method for large-scale propagation of V. baoshanensis To test this hypothesis, three types of explants were used for shoot regeneration and various combinations of four plant growth regulators were used to improve shoot regeneration efficiency from leaflet of V. baoshanensis. Best shoot regeneration efficiency was obtained by incubating leaflet in a 1/2 MS medium supplemented with 2.5 oM BA + 2.5 microM IBA, therein shoot regeneration rate was 70.9% and the number of shoots formation per explant was 22.4. Rooting was achieved from almost all regenerated shoot growing on 1/2 MS medium without plant growth regulator. Micropropagated seedlings were acclimatized under greenhouse conditions and 95% of them survived and showed no visible morphological variation compared to their donor plant. Furthermore, there were no significant differences between regenerated and seed-germinated V. baoshanensis in Cd tolerance and accumulation. These results suggested that an efficient and rapid micropropogation system was successfully developed for V. baoshanensis.

  8. Micropropagation of Asparagus by in vitro shoot culture.

    Science.gov (United States)

    Stajner, Nataša

    2013-01-01

    Asparagus officinalis is most extensively studied species within the genus Asparagus, which is well known as garden asparagus. This species is dioecious with unisexual flowers, which means that generative propagation gives roughly equal number of male and female plants. Male plants are high yielders and preferred commercially over female plants. Tissue culture techniques could efficiently promote vegetative propagation of male plants and pave the way for efficient plant breeding.This chapter describes an efficient micropropagation protocol for developing rapid growing in vitro Asparagus shoot cultures. The source of explants, inoculation, and shoot proliferation, followed by shoot propagation, rooting, and acclimatization is described. The optimal medium for Asparagus micropropagation described in this chapter is composed of MS macro- and microelements and a combination of auxins and cytokinins. Plant growth regulators NAA, kinetin, and BA were used in various concentrations. Three different media representing the whole micropropagation protocol of Asparagus are described; medium for shoot initiation, medium for shoot multiplication, and medium for root formation. By in vitro propagation of Asparagus, root initiation is difficult, but can be promoted by adding growth retardant ancymidol which also greatly promotes shoot development and suppresses callus formation.

  9. Micropropagation of tulip: production of virus-free stock plants.

    Science.gov (United States)

    Podwyszyńska, Małgorzata; Sochacki, Dariusz

    2010-01-01

    We describe here a new tulip micropropagation method based on the cyclic shoot multiplication in presence of the thidiazuron (TDZ), which enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. In our novel protocol, cyclic shoot multiplication can be performed for 2-3 years by using TDZ instead of other cytokinins, as 6-benzylaminopurine (BAP) and N(6)-(-isopentyl)adenine (2iP). It makes possible to produce 500-2,000 microbulbs from one healthy plant. There are six main stages of tulip micropropagation. Stage 0 is the selection of true-to-type and virus-free plants, confirmed by ELISA. Fragments of flower stems isolated from bulbs are used as initial explants. Shoot multiplication is based on the regeneration of adventitious shoots, which are sub-cultured every 8 weeks. In the Stage 3, the specially prepared shoots are induced by low temperature treatment to form bulbs which finally develop on a sucrose-rich medium at 20 degrees C. Bulbs are then dried for 6 weeks and rooted in vivo. The number of multiplication subcultures should be limited to 5-10 cycles in order to lower the risk of mutation. Virus indexing should be repeated 3-4 times, at the initial stage and then during shoot multiplication. Genetic stability of micropropagated shoots can be confirmed using molecular markers.

  10. Analyzing clonal fidelity of micropropagated Psidium guajava L. plants using simple sequence repeat markers

    Science.gov (United States)

    Micropropagation of Psidium guajava L. (guava) is a viable alternative to currently adopted techniques for large-scale plant propagation of commercial cultivars. Assessment of clonal fidelity in micropropagated plants is the first step towards ensuring genetic uniformity in mass production of planti...

  11. A REVIEW: MICROPROPAGATION OF PHALAENOPSIS sp FROM LEAF AND FLOWER STALK EXPLANTS

    Directory of Open Access Journals (Sweden)

    Meutia Zahara

    2017-09-01

    • Semiarti, E.; Indrianto, A.; Purwanto, A. Agrobacterium-Mediated transformation of Indonesian orchids for  micropropagation, genetic transformation, Prof. MarÃa Alvarez (Ed., ISBN: 978-953-307-364-4, InTech, 2011. Available from: http://www.intechopen.com/books/ genetic-transformation/agrobacterium-mediated-transformation-ofindonesian-orchids-for-micropropagation.

  12. Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers.

    Science.gov (United States)

    Siril, E A; Joseph, Nisha

    2013-01-01

    An in vitro propagation technique based on axillary bud proliferation was developed for the first time to mature annatto (Bixa orellana L.) tree. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with 1.0 μM benzyl adenine (BA) and tender coconut water (10 %) showed significantly high (P micropropagated plants. The present protocol in brief, can be used for the clonal propagation of the superior genotype and preservation of germplasm.

  13. Microbiological and Pharmacological Evaluation of the Micropropagated Rubus liebmannii Medicinal Plant

    Directory of Open Access Journals (Sweden)

    Adelina Jiménez-Arellanes

    2012-01-01

    Full Text Available Rubus liebmannii is an endemic species from Mexico used in traditional medicine primarily to treat dysentery and cough. The in vitro activity against Giardia lamblia and Entamoeba histolytica that produces the ethanolic extract of the aerial parts of the plant led us to expand the pharmacological and phytochemical research of this species. Gastrointestinal disorders including amebiasis remain one of the health problems that need to be addressed and it is of interest to find alternatives that improve their treatment. Also, it is important to emphasize that R. liebmannii grows wild in the country and is not found in abundance; therefore, alternatives that avoid overexploitation of the natural resource are mandatory. Ongoing with the evaluation of the potentialities that R. liebmannii possesses for treating infectious gastrointestinal diseases, the aim of the present study was to evaluate the biological effects and the chemical composition of the micropropagated plant.

  14. Modeling and Explaining Content: Definition, Research Support, and Measurement of the "ETS"® National Observational Teaching Examination (NOTE) Assessment Series. Research Memorandum No. RM-16-07

    Science.gov (United States)

    Stickler, Leslie; Sykes, Gary

    2016-01-01

    This report reviews the scholarly and research evidence supporting the construct labeled modeling and explaining content (MEC), which is measured via a performance assessment in the "ETS"® National Observational Teaching Examination (NOTE) assessment series. This construct involves practices at the heart of teaching that deal with how…

  15. Eliciting Student Thinking: Definition, Research Support, and Measurement of the "ETS"® National Observational Teaching Examination (NOTE) Assessment Series. Research Memorandum No. RM-16-06

    Science.gov (United States)

    Qi, Yi; Sykes, Gary

    2016-01-01

    This report describes and provides research and scholarly support for a core practice of teaching--eliciting student thinking (EST)--that is the target for a performance assessment contributing one component of the "ETS"® National Observational Teaching Examination (NOTE) assessment series. The purpose of this report is to review the…

  16. Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

    Directory of Open Access Journals (Sweden)

    Nair S. Yokoya

    2011-05-01

    Full Text Available Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta. Micropropagation, or in vitro clonal propagation, allows the production of a large number of individuals within a short period. These micropropagated clones could be used as seedlings for seaweed cultivation, avoiding collection from natural beds. Consequently, there has been an increasing interest in micropropagation as a tool for preservation of populations of marine red algae on the Brazilian coast and for the sustainable production of raw material for commercial exploration. This paper reviews the literature on tissue culture and micropropagation of red algae published during the three last decades. Based on the literature, we can conclude that the regeneration process is complex and diversified in different species of Rhodophyta and that the success of micropropagation depends on this process. Species belonging to the orders Bangiales and Ceramiales showed low potential for regeneration, while Gigartinales species showed the highest potential for regeneration. Micropropagation of commercially important red algae is fundamental for the conservation of natural populations by providing seedlings for cultivation and for germplasm collections, both for the conservation of genetic diversity and for biotechnological applications.

  17. A note on the Pazos-Simonsen mechanism and Kaldor's early research on Latin American inflation

    Directory of Open Access Journals (Sweden)

    Leonardo Vera

    2013-06-01

    Full Text Available In contrast with the inflationary finance story, inflation acceleration in Latin America has been explained as the result of the interaction of inflation dynamics and the frequency of wage adjustments. Accordingly, small inflation disturbances are connected with a shift from moderate to high inflation (or beyond to hyperinflation though a mechanism that makes adjustment intervals in wage contracts endogenous. Rudiger Dornbusch (1986 labeled this process the "Pazos-Simonsen mechanism". In this note we summarize the basic contribution of both Felipe Pazos (1978 and Mario Henrique Simonsen (1983 and find crucial differences between their views on wage dynamics, specifically regarding the endogeneity of the time interval between wage readjustments. A remarkable affinity with Pazos's view on wage dynamics and inflation is found in an early and almost unknown essay written by Nicholas Kaldor in 1957 (inspired in his brief experience in Latin America.

  18. Micropropagation to Rescue Endangered Plant Moringa Concanensis Nimmo (Moringaceae)

    International Nuclear Information System (INIS)

    Fatima, H.

    2016-01-01

    Efficient micropropagation was developed for an endangered plant; Moringa concanensis Nimmo. The plant has many medicinal properties. It is an antioxidant, anti-diabetic and a powerful tonic alternative. A high frequency and maximum number of shoots were produced in MS supplemented with the combination of Benzyl aminopurine (BAP) and Alpha-Naphthalene Acetic Acid (NAA) (0.10mg/l + 0.05mg/l). Rooting was achieved by the inoculation of regenerated shoots on half strength MS without addition of hormone. (author)

  19. The Interface between Research and Policy--A Note with Potential Relevance for Higher Education

    Science.gov (United States)

    Gornitzka, Åse

    2013-01-01

    The nexus between research and policy in higher education as in other sectors of society is multimodal and not adequately captured by the notion of a cultural gap between the world of practice and the world of research. Neither can the relationship be seen as unidirectional. This paper sketches out the range of uses of research in policy-making,…

  20. [Historical notes about scientific research in the Instituto Mexicano del Seguro Social.

    Science.gov (United States)

    Zárate, Arturo; Basurto-Acevedo, Lourdes

    2013-01-01

    Medical research in the Instituto Mexicano del Seguro Social has been considered one of the most important in this country for quality and quantity. Thanks to the work and leadership of Benito Coquet, who initiated the building of the National Medical Center in 1961, and the work of two pillars of research, Luis Castelazo and Bernardo Sepúlveda, the Institute successfully improved scientific research. In the years that followed, the Institute fostered the professionalization of research, the creation of research units in different areas of science, the incorporation of consolidated groups of researchers, the relationship with other institutions, the incorporation to the Sistema Nacional de Investigadores, the editing of a journal to expose outside the work done within the Institute, and the formation of a trust to raise funds for financing. Thanks to all that, institutional research strengthened in all lines, and it was placed first, at certain times, at the national level.

  1. Predictors of article impact in suicidology: the bereavement literature, a research note.

    Science.gov (United States)

    Andriessen, Karl; Krysinska, Karolina; Stack, Steven

    2015-02-01

    Citation analysis has been neglected in suicidology. The present note applies a mixed-methods approach to both test and suggest hypotheses for the variation in article impact in the bereavement literature. One hundred three articles from three core suicidology journals met the criteria for inclusion in the investigation. Citations to the articles were obtained from the Web of Science. Predictor variables included structural characteristics of the author (e.g., gender) and the article itself (e.g., years since publication). A multivariate regression analysis determined that, controlling for the other variables, the most important predictor of citations was the review article (β = .461), followed by year of publication (β = -.414), the multiauthored article (β = .302), publication in Suicide and Life-Threatening Behavior (SLTB) (β = .161), and male gender (β = .156). The 12 most cited articles were published between 1979 and 2004 in SLTB. The majority of these papers was written by males, were U.S. authors, and had more than one author. Four of the most cited articles were reviews. The study concludes that structural characteristics of articles and authors explained 41% of the variance in citations. The qualitative analysis determined that review papers, and papers on characteristics of suicide bereavement and psychological autopsies have been most frequently cited. Replication studies are needed for other subfields of suicidology. © 2014 The American Association of Suicidology.

  2. Research Notes: Young People Face Issues of Self-Esteem and Child Abuse.

    Science.gov (United States)

    Jordan, Debra J.

    1994-01-01

    Reviews current research on the effects of physical appearance on children's self-esteem and research on posttraumatic stress syndrome in abused children. Implications for staff at youth camps include discouraging children from ridiculing others and being alert to the signs and symptoms of posttraumatic stress disorder. (LP)

  3. Coping Strategies Used by School Staff after a Crisis: A Research Note

    Science.gov (United States)

    Cole, Rachel; Hayes, Ben; Jones, Dan; Shah, Sonia

    2013-01-01

    There is much literature on crisis support in schools but little on how school staff are affected. This research had two aims: to begin to explore the coping strategies used by school staff after a crisis event, and to investigate measures that might prove valuable for future research. Seven cases are presented using three measures: the WHO (Five)…

  4. Teaching Note--"By Any Means Necessary!" Infusing Socioeconomic Justice Content into Quantitative Research Course Work

    Science.gov (United States)

    Slayter, Elspeth M.

    2017-01-01

    Existing research suggests a majority of faculty include social justice content in research courses but not through the use of existing quantitative data for in-class activities that foster mastery of data analysis and interpretation and curiosity about social justice-related topics. By modeling data-driven dialogue and the deconstruction of…

  5. IS Research and Policy: Notes From the 2015 ICIS Senior Scholar’s Forum

    DEFF Research Database (Denmark)

    Niederman, Fred; Applegate, Lynda; Beck, Roman

    2017-01-01

    Based on the International Conference on Information Systems’ (ICIS) 2015 senior scholars’ forum, we provide insights on the role and opportunities of IS researchers in shaping policy.......Based on the International Conference on Information Systems’ (ICIS) 2015 senior scholars’ forum, we provide insights on the role and opportunities of IS researchers in shaping policy....

  6. The Economics of Pricing and Customer Decision-Making. Research Notes.

    Science.gov (United States)

    Jordan, Debra J.

    1996-01-01

    Reviews two research articles. Gratton and Taylor, using economic analysis and market research, found that consumers of leisure experiences complained about price increases, but continued consuming. They recommend discounts for those in need and price increases focusing on service quality. Greenleaf and Lehmann identified 11 reasons why people…

  7. A novel method for coral explant culture and micropropagation.

    Science.gov (United States)

    Vizel, Maya; Loya, Yossi; Downs, Craig A; Kramarsky-Winter, Esti

    2011-06-01

    We describe here a method for the micropropagation of coral that creates progeny from tissue explants derived from a single polyp or colonial corals. Coral tissue explants of various sizes (0.5-2.5 mm in diameter) were manually microdissected from the solitary coral Fungia granulosa. Explants could be maintained in an undeveloped state or induced to develop into polyps by manipulating environmental parameters such as light and temperature regimes, as well as substrate type. Fully developed polyps were able to be maintained for a long-term in a closed sea water system. Further, we demonstrate that mature explants are also amenable to this technique with the micropropagation of second-generation explants and their development into mature polyps. We thereby experimentally have established coral clonal lines that maintain their ability to differentiate without the need for chemical induction or genetic manipulation. The versatility of this method is also demonstrated through its application to two other coral species, the colonial corals Oculina patigonica and Favia favus.

  8. Somatic embryogenesis for efficient micropropagation of guava (Psidium guajava L.).

    Science.gov (United States)

    Akhtar, Nasim

    2013-01-01

    Guava (Psidium guajava L.) is well known for edible fruit, environment friendly pharmaceutical and commercial products for both national and international market. The conventional propagation and in vitro organogenesis do not meet the demand for the good quality planting materials. Somatic embryogenesis for efficient micropropagation of guava (P. guajava L.) has been developed to fill up the gap. Somatic embryogenesis and plantlets regeneration are achieved from 10-week post-anthesis zygotic embryo explants by 8-day inductive treatment with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) on MS agar medium containing 5% sucrose. Subsequent development and maturation of somatic embryos occur after 8 days on MS basal medium supplemented with 5% sucrose without plant growth regulator. The process of somatic embryogenesis shows the highest relative efficiency in 8-day treatment of zygotic embryo explants with 1.0 mg L(-1) 2,4-D. High efficiency germination of somatic embryos and plantlet regeneration takes place on half strength semisolid MS medium amended with 3% sucrose within 2 weeks of subculture. Somatic plantlets are grown for additional 2 weeks by subculturing in MS liquid growth medium containing 3% sucrose. Well-grown plantlets from liquid medium have survived very well following 2-4 week hardening process. The protocol of somatic embryogenesis is optimized for high efficiency micropropagation of guava species.

  9. Musica de la Frontera: Research Note on the UCLA Frontera Digital Archive

    Science.gov (United States)

    Romero, Robert Chao

    2005-01-01

    The Frontera Digital Archive is an impressive and invaluable research tool for multidisciplinary scholars of Chicana/o studies and Latin American studies. The archive preserves rare Mexican vernacular musical recordings and provides convenient access to these recordings via Internet.

  10. Note On Research Design For The Study Of Community Participation In Health Care Programmes

    Directory of Open Access Journals (Sweden)

    Rifkin Susan B

    1986-01-01

    Full Text Available After describing types of research designs for the study of community participation in health care programmes, this paper examines one methodology, the quantitative methodology, the quantitative methodology, in detail. It presents some of the major attractions and limitations of this approach. The attractions include the need for evaluation of success and failure and of cost effectiveness of programmes. The limitations include the inability of the approach to deal with definitions and interventions that cannot be quantitified and the difficulty of identifying casual relationship between interventions and outcomes. These characteristics are illustrated by a case by a medical school in Asia. Research design, research developments and research outcomes are described and analysed. The paper concludes that an alternative analysis which examines the linkages between participation and health improvements would be more useful as it would allow the political, social and economic dimensions of community participation to be examined.

  11. Research notes : drainage facility asset management : more than an inventory of pipes.

    Science.gov (United States)

    2007-04-01

    The primary objectives for the research project were twofold: 1) To develop and implement an Oregon-specific system for inventorying and evaluating the condition of pipes, culverts, and stormwater facilities based on the FHWA Culvert Management Syste...

  12. Research Note: The consequences of different methods for handling missing network data in Stochastic Actor Based Models.

    Science.gov (United States)

    Hipp, John R; Wang, Cheng; Butts, Carter T; Jose, Rupa; Lakon, Cynthia M

    2015-05-01

    Although stochastic actor based models (e.g., as implemented in the SIENA software program) are growing in popularity as a technique for estimating longitudinal network data, a relatively understudied issue is the consequence of missing network data for longitudinal analysis. We explore this issue in our research note by utilizing data from four schools in an existing dataset (the AddHealth dataset) over three time points, assessing the substantive consequences of using four different strategies for addressing missing network data. The results indicate that whereas some measures in such models are estimated relatively robustly regardless of the strategy chosen for addressing missing network data, some of the substantive conclusions will differ based on the missing data strategy chosen. These results have important implications for this burgeoning applied research area, implying that researchers should more carefully consider how they address missing data when estimating such models.

  13. Human Participants in Engineering Research: Notes from a Fledgling Ethics Committee.

    Science.gov (United States)

    Koepsell, David; Brinkman, Willem-Paul; Pont, Sylvia

    2015-08-01

    For the past half-century, issues relating to the ethical conduct of human research have focused largely on the domain of medical, and more recently social-psychological research. The modern regime of applied ethics, emerging as it has from the Nuremberg trials and certain other historical antecedents, applies the key principles of: autonomy, respect for persons, beneficence, non-maleficence, and justice to human beings who enter trials of experimental drugs and devices (Martensen in J Hist Med Allied Sci 56(2):168-175, 2001). Institutions such as Institutional Review Boards (in the U.S.) and Ethics Committees (in Europe and elsewhere) oversee most governmentally-funded medical research around the world, in more than a hundred nations that are signers of the Declaration of Helsinki (World Medical Association 2008). Increasingly, research outside of medicine has been recognized to pose potential risks to human subjects of experiments. Ethics committees now operate in the US, Canada, the U.K. and Australia to oversee all governmental-funded research, and in other jurisdictions, the range of research covered by such committees is expanding. Social science, anthropology, and other fields are falling under more clear directives to conduct a formal ethical review for basic research involving human participants (Federman et al. in Responsible research: a systems approach to protecting research participants. National Academies Press, Washington, 2003, p. 36). The legal and institutional response for protecting human subjects in the course of developing non-medical technologies, engineering, and design is currently vague, but some universities are establishing ethics committees to oversee their human subjects research even where the experiments involved are non-medical and not technically covered by the Declaration of Helsinki. In The Netherlands, as in most of Europe, Asia, Latin America, or Africa, no laws mandate an ethical review of non-medical research. Yet, nearly 2

  14. Agricultural biotechnology research and development in Ethiopia

    African Journals Online (AJOL)

    PRECIOUS

    2009-12-29

    Dec 29, 2009 ... Review. Agricultural biotechnology research and development in Ethiopia ... seed micropropagation, virus-cleaning ongoing, good progress. Garlic meristem ... large quantities of disease-free planting materials in short time.

  15. Micropropagation of Origanum acutidens (HAND.-MAZZ.) IETSWAART using stem node explants.

    Science.gov (United States)

    Yildirim, Mehmet Ugur

    2013-01-01

    Origanum acutidens (HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0.6, 1.2, 1.8, and 2.4 mg/L BAP with 0.2 mg/L NAA. Visible effects of culture media on shoot proliferation were recorded. Shoot regeneration rate was maximum on MS medium containing 1.80 mg/L BAP-0.2 mg/L NAA. The micropropagated shoots were rooted on MS medium containing 0.2 mg/L NAA. All microrooted plantlets survived during acclimatisation on peat moss. It was concluded that O. acutidens can be successfully micropropagated under in vitro conditions.

  16. Rapid micro-propagation of Aloe vera L. via shoot multiplication

    African Journals Online (AJOL)

    STORAGESEVER

    2008-06-17

    . Cloning protocol of Aloe vera as a study-case for tailor- made biotechnology to small farmers. J. Technol. Manag. Innov. 1: 76-79. Debiasi C, Silva CG, Pescador R (2007). Micropropagation of Aloe vera. L., Rev. Bras. PIant.

  17. Micropropagation of Oenothera biennis L. and an assay of fatty acids

    Directory of Open Access Journals (Sweden)

    Lutosława Skrzypczak

    2014-01-01

    Full Text Available The species Oenothera biennis L. was micropropagated in in vitro cultures. After transferring to the ground plants developed correctly and generated fruits with seeds. The content of oil and γ-linolenic acid in seeds was determined.

  18. Updating AP Potential™ Expectancy Tables Involving PSAT/NMSQT® Writing. Research Notes. RN-35

    Science.gov (United States)

    Ewing, Maureen; Camara, Wayne J.; Millsap, Roger E.; Milewski, Glenn B.

    2007-01-01

    AP Potential™ is a data-driven tool offered by the College Board that uses scores from the PSAT/NMSQT® to identify students who have the potential to succeed in Advanced Placement Program® (AP®) courses (College Board, 2007). Research showing a moderate-to-strong correlation between PSAT/NMSQT scores and AP Exam scores serves as the basis for this…

  19. Acoustics in Research Facilities--Control of Wanted and Unwanted Sound. Laboratory Design Notes.

    Science.gov (United States)

    Newman, Robert B.

    Common and special acoustics problems are discussed in relation to the design and construction of research facilities. Following a brief examination of design criteria for the control of wanted and unwanted sound, the technology for achieving desired results is discussed. Emphasis is given to various design procedures and materials for the control…

  20. Technical Note: Harmonizing met-ocean model data via standard web services within small research groups

    Science.gov (United States)

    Signell, Richard; Camossi, E.

    2016-01-01

    Work over the last decade has resulted in standardised web services and tools that can significantly improve the efficiency and effectiveness of working with meteorological and ocean model data. While many operational modelling centres have enabled query and access to data via common web services, most small research groups have not. The penetration of this approach into the research community, where IT resources are limited, can be dramatically improved by (1) making it simple for providers to enable web service access to existing output files; (2) using free technologies that are easy to deploy and configure; and (3) providing standardised, service-based tools that work in existing research environments. We present a simple, local brokering approach that lets modellers continue to use their existing files and tools, while serving virtual data sets that can be used with standardised tools. The goal of this paper is to convince modellers that a standardised framework is not only useful but can be implemented with modest effort using free software components. We use NetCDF Markup language for data aggregation and standardisation, the THREDDS Data Server for data delivery, pycsw for data search, NCTOOLBOX (MATLAB®) and Iris (Python) for data access, and Open Geospatial Consortium Web Map Service for data preview. We illustrate the effectiveness of this approach with two use cases involving small research modelling groups at NATO and USGS.

  1. Micropropagation of Launaea cornuta - an important indigenous ...

    African Journals Online (AJOL)

    FAITH AMBAJO

    2016-08-10

    Aug 10, 2016 ... MATERIALS AND METHODS. Establishment of plants in the ... sterile soil in plastic bags and placed in the screen house at Kabete. Biotechnology Center of Kenya Agricultural and Livestock Research. Organization (KALRO).

  2. Research Notes ~ Second Language Acquisition Theories as a Framework for Creating Distance Learning Courses

    Directory of Open Access Journals (Sweden)

    Eileen N. Ariza

    2003-10-01

    Full Text Available Moore and Kearsley (1996 maintain distance educators should provide for three types of interaction: a learner-content; b learner-instructor; and c learner-learner. According to interactionist second language acquisition (SLA theories that reflect Krashen’s theory (1994 that comprehensible input is critical for second language acquisition, interaction can enhance second language acquisition and fluency. Effective output is necessary as well. We reviewed the research on distance learning for second language learners and concluded that SLA theories can, and should, be the framework that drives the development of courses for students seeking to learn languages by distance technology. This article delineates issues to consider in support of combining SLA theories and research literature as a guide in creating distance language learning courses.

  3. Social theory and Brazilian social thought: notes for a research agenda

    Directory of Open Access Journals (Sweden)

    Marcelo Maia

    2010-01-01

    Full Text Available So-called "social thought" has always occupied a prominent place in the social sciences in Brazil. Current research in the field has increasingly sought to articulate in its analysis of national essayistic production broader theoretical preoccupations regarding the status of modernity in non-central societies. Taking as its starting point this intellectual state of affairs, this article seeks to accomplish two principal goals: a justify the need for a dialogue between Brazilian social thought and social theory, in particular post-colonial theories and criticism of the Eurocentric tradition in sociology; b explore possible further points of dialogue between these areas through an examination of analytical nexuses common to both fields of research.

  4. Political learning in territories of exclusion, conflict and popular organization (research notes)

    OpenAIRE

    Paulo J. Krischke

    2006-01-01

    This paper discusses some interfaces between the approaches to political learning, and their reference to situations of social exclusion, conflict and popular organization. The first part of the paper discusses the approach to the study of political learning among the elites; the second part examines approaches to research of political culture among the masses; and the third part outlines an alternative approach to political learning derived from Jürgen Habermass theory of comm...

  5. Mentoring and turnover intentions in public accounting firms: a research note

    OpenAIRE

    Matthew Hall; David Smith

    2009-01-01

    Turnover in public accounting firms is a critically important issue as firms seek to retain quality accounting personnel in the face of skilled labour shortages. Mentoring is one initiative that has been suggested as a means of reducing the high costs associated with employee turnover. However, prior accounting research examining the association between mentoring and turnover intentions has produced mixed results, which may be due, at least in part, to difficulties in operationalizing the men...

  6. Technical Note: DIRART- A software suite for deformable image registration and adaptive radiotherapy research

    International Nuclear Information System (INIS)

    Yang Deshan; Brame, Scott; El Naqa, Issam; Aditya, Apte; Wu Yu; Murty Goddu, S.; Mutic, Sasa; Deasy, Joseph O.; Low, Daniel A.

    2011-01-01

    Purpose: Recent years have witnessed tremendous progress in image guide radiotherapy technology and a growing interest in the possibilities for adapting treatment planning and delivery over the course of treatment. One obstacle faced by the research community has been the lack of a comprehensive open-source software toolkit dedicated for adaptive radiotherapy (ART). To address this need, the authors have developed a software suite called the Deformable Image Registration and Adaptive Radiotherapy Toolkit (DIRART). Methods: DIRART is an open-source toolkit developed in MATLAB. It is designed in an object-oriented style with focus on user-friendliness, features, and flexibility. It contains four classes of DIR algorithms, including the newer inverse consistency algorithms to provide consistent displacement vector field in both directions. It also contains common ART functions, an integrated graphical user interface, a variety of visualization and image-processing features, dose metric analysis functions, and interface routines. These interface routines make DIRART a powerful complement to the Computational Environment for Radiotherapy Research (CERR) and popular image-processing toolkits such as ITK. Results: DIRART provides a set of image processing/registration algorithms and postprocessing functions to facilitate the development and testing of DIR algorithms. It also offers a good amount of options for DIR results visualization, evaluation, and validation. Conclusions: By exchanging data with treatment planning systems via DICOM-RT files and CERR, and by bringing image registration algorithms closer to radiotherapy applications, DIRART is potentially a convenient and flexible platform that may facilitate ART and DIR research.

  7. Research Paper:

    African Journals Online (AJOL)

    Shanu

    2014-01-08

    Jan 8, 2014 ... Forty years in capsaicin research for sensory pharmacology and physiology. Neuropeptides 38:377-384. Thomas E (2002). Tissue culture studies in Arachis hypogea L. and. Vignaunguiculata (L.) Walp. for micropropagation and cell line selection for amino acid overproduction, Ph.D. Thesis, University of.

  8. Psychological adjustment of Yoruba adolescents as influenced by family type: a research note.

    Science.gov (United States)

    Oyefeso, A O; Adegoke, A R

    1992-05-01

    This research examines the influence of family type on the psychological adjustment of Yoruba adolescents. Using a sample of 116 adolescents, 69 males and 47 females, with mean age of 17.8 years of age (S.D. = 1.72), the results reveal that male adolescents from monogamous families experience better psychological adjustment than their polygynous counterparts, whereas no such difference exists in the levels of psychological adjustment of female adolescents from both family types. These findings suggest that (i) sex-role prescription influences psychological adjustment of adolescents in Yoruba societies, and (ii) female children enjoy more protective upbringing in polygynous families than their male counterparts.

  9. Shoestrings and bricolage: some notes on researching the impact of a child's death on family relationships.

    Science.gov (United States)

    Riches, Gordon; Dawson, Pam

    2002-04-01

    An earlier version of this paper appeared as the appendix to our recent book, An Intimate Loneliness: Supporting Bereaved Parents and Children (G. Riches & P. Dawson, 2000). In it, we attempt to offer a brief history of the processes we have gone through in taking a simple research question, developing it into a practical proposal, experiencing how it shifted as we explored it, accounting for the changes that our broader reading imposed on our perceptions of what we were doing, and, finally, how the data itself and our efforts to understand it, resulted in a set of broad theoretical propositions rather than any tight conclusions.

  10. Exercise in completing design information questionnaire for model research reactor: model description, notes, questionnaire

    International Nuclear Information System (INIS)

    Bellinger, J.; Ho, T.

    1989-01-01

    The document which defines the inspection measures which the IAEA can deploy at any given nuclear facility is known as the Facility Attachment. For the Agency to negotiate an effective Facility Attachment it must have available certain design information, including the facility's identity, capacity and location; the form, location and flow of nuclear material and the layout of important items of equipment; and a description of the features and procedures relating to nuclear material accountancy, containment and surveillance. In practice such information is solicited in a format, standardized for each facility type, known as the Design Information Questionnaire or the D.I.Q. The nuclear activities used as a model in this course are those of a fictitious country called Pacifica. These nuclear activities bear some resemblance to those at the Australian Atomic Energy Commission's Research Establishment at Lucas Heights. Specifically, Pacifica has a 10 MW heavy water cooled and moderated research reactor using enriched uranium fuel which is very similar to the HIFAR reactor. The reactor and the associated laboratories are described and the Design Information Questionnaire for them is completed. figs., tabs

  11. Notes on Contributors

    African Journals Online (AJOL)

    NOTES ON CONTRIBUTORS. Samuel AMOAKO, Associate Researcher, South African Research Unit in Social Change. Contact Details: C/o Lucinda Bercony, Humanities Research Village (House No. 3). University of Johannesburg, P. O. Box 524. Bunting Road Campus, Auckland Park, 2006.

  12. Notes on Women Who Rock: Making Scenes, Building Communities: Participatory Research, Community Engagement, and Archival Practice

    Directory of Open Access Journals (Sweden)

    Michelle Habell-Pallán

    2014-07-01

    Full Text Available Since 2011, Women Who Rock (WWR has brought together scholars, archivists, musicians, media-makers, performers, artists, and activists to explore the role of women and popular music in the creation of cultural scenes and social justice movements in the Americas and beyond. The project promotes generative dialogue and documentation by “encompassing several interwoven components: project-based coursework at the graduate and undergraduate levels; an annual participant-driven conference and film festival; and an oral history archive hosted by the University of Washington Libraries Digital Initiatives Program that ties the various components together” (Bartha 8. In our courses, programming, and archive, we examine the politics of performance, social identity, and material access in music scenes, cultures, and industries. Performance studies scholar Daphne Brooks argues that the “confluence of cultural studies, rock studies, and third wave feminist critical studies makes it possible now more than ever to continue to critique and re-interrogate the form and content of popular music histories” (58. WWR implements this approach, asking how particular stories of popular music determine a performer, band, or scene’s “legendary” status or excision from the official annals of memory. WWR reshapes conventional understandings of popular music studies by initiating collective methods of participatory research, as well as community collaboration and dialogue. By way of WWR, we seek to transform traditional models of popular music studies, instigating new convergences between academic disciplines and critical approaches that create alternative histories and new forms of knowledge.

  13. Micropropagation and cytogenetic assessment of Zingiber species of Northeast India.

    Science.gov (United States)

    Das, Archana; Kesari, Vigya; Rangan, Latha

    2013-12-01

    An improved micropropagation protocol was developed for Zingiber moran and Z. zerumbet, two wild species of the genus Zingiber, found in Northeast India. The effects of growth regulators, sugar concentrations, and nutrients were tested on the rate of shoot initiation and multiplication. An increase in proliferation and multiplication occurred in modified Murashige and Skoog (MS) medium supplemented with benzyladenine and kinetin. About 2 % sucrose and 0.7 % agar were found to be the optimum for shoot multiplication and regeneration. Naphthalene acetic acid at 0.5 mg/L produced the best rooting response for both the species. Regenerated plantlets were acclimatized successfully and cytogenetic stability was confirmed by RAPD profiling and ploidy checks.

  14. Micropropagation and maintenance of phytoplasmas in tissue culture.

    Science.gov (United States)

    Bertaccini, Assunta; Paltrinieri, Samanta; Martini, Marta; Tedeschi, Mara; Contaldo, Nicoletta

    2013-01-01

    Maintenance of phytoplasma strains in tissue culture is achievable for all strains transmitted to periwinkle (Catharanthus roseus), and also for other naturally infected plant host species. Shoots of 1-3 cm length are grown in a solid medium containing Murashige and Skoog (MS) micro- and macroelements and 0.12 mg/L benzylaminopurine. The continued presence of phytoplasmas in infected shoots of periwinkle that have been maintained in micropropagation for up to 20 years can be shown by diagnostic methods such as nested PCR tests using the 16S rDNA gene (see Chapters 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,and 26 for phytoplasma diagnostic methods).

  15. Micropropagation of Vaccinium sp. by in vitro axillary shoot proliferation.

    Science.gov (United States)

    Litwińczuk, Wojciech

    2013-01-01

    The Vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (Vaccinium × corymbosum L.), cranberry (Vaccinium macrocarpon Ait.), and lingonberry (Vaccinium vitis-idaea L.). In some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants could be fulfilled only by micropropagation. In principle cultivars are propagated in vitro through similar three-stage method, based on subculture of shoot explants on different culture media supplemented with IAA (0-4 mg/L) and 2iP (5-10 mg/L), and rooting shoots in vivo. The obtained plantlets are transferred to peat substrate and grown in the glasshouse until the end of growing period. The development of adventitious shoots should be monitored and controlled during in vitro stages. Many clones have specific requirements for growing conditions and/or are recalcitrant.

  16. Micropropagation of Citrus spp. by organogenesis and somatic embryogenesis.

    Science.gov (United States)

    Chiancone, Benedetta; Germanà, Maria Antonietta

    2013-01-01

    Citrus spp., the largest fruit crops produced worldwide, are usually asexually propagated by cuttings or grafting onto seedling rootstocks. Most of Citrus genotypes are characterized by polyembryony due to the occurrence of adventive nucellar embryos, which lead to the production of true-to-type plants by seed germination. Tissue culture and micropropagation, in particular, are valuable alternatives to traditional propagation to obtain a high number of uniform and healthy plants in a short time and in a small space. Moreover, in vitro propagation provides a rapid system to multiply the progeny obtained by breeding programs, allows the use of monoembryonic and seedless genotypes as rootstocks, and it is very useful also for breeding and germplasm preservation.In this chapter, two protocols regarding organogenesis of a rootstock and somatic embryogenesis of a cultivar have been described.

  17. In vitro micropropagation of chive (Allium schoenoprasum L.

    Directory of Open Access Journals (Sweden)

    Marek Lubomski

    2013-12-01

    Full Text Available The investigations on micropropagation of Allium schoenoprasum embraced shoot formation from cultured shoot tips, shoot multiplication, root formations, and cold storage of rooted plants. All explants were cultured on modified Murashige-Skoog medium. Surface disinfection of shoots derived from stock plants was not necessary to obtain no infection culture. The highest shoot multiplication was obtained on medium with 20 g l-1 sucrose and 1.0 mg 1-1 6-benzylo-arninopurine (BA. Root formation was observed in both indoleacetic acid (IAA and indolebutyric acid (IBA. Rooted plants were successfully kept in cold storage (5°C ± 1°C darkness. Only 20% of plants died after 6 months of storage.

  18. Micropropagation of Prunus species relevant to cherry fruit production.

    Science.gov (United States)

    Druart, Philippe

    2013-01-01

    Cherry tree micropropagation is limited to the production of healthy cultivars of Prunus avium and Prunus cerasus, and their rootstocks; mainly the dwarfing ones. By using meristem-tip (0.1 mm long) or healthy shoot tips/nodes, four successive steps are needed to obtain whole plants capable of growing in the nursery: multiplication by axillary branching, shoot elongation, rooting, and plantlet acclimation. Along this process, several parameters have to be adjusted for each phase of the culture, including media composition, environmental culture conditions and plant handling. These parameters vary depending on genotypic response and specific vulnerability to physiological disorders such as hyperhydricity, apex necrosis, unstable propagation, and rooting rates. Based on a 40 year-long experience of study and application of culture conditions to large-scale plant production, this document summarizes the main problems (variability of the propagation rate, hyperhydricity, apex necrosis, plant re-growth) and solutions encountered to solve them, with means validated on many mericlones.

  19. Micropropagation of six Paulownia genotypes through tissue culture

    Directory of Open Access Journals (Sweden)

    Lydia Shtereva

    2014-12-01

    Full Text Available We investigated the effect of genotype and culture medium on the in vitro germination and development of plantlets from seeds of 6 different Paulownia genotypes (P. tomentosa, hybrid lines P. tomentosa P. fortunei (Mega, Ganter and Caroline, P. elongata and hybrid line P. elongata P. fortunei. Nodal and shoot tip explants were used for micropropagation of Paulownia genotypes by manipulating plant growth regulators. The highest germination percentage for all genotypes was obtained for seeds inoculated on medium supplemented with 50 mg*L GA3 (MSG2. On Thidiazuron containing media, the explants of hybrid line P. elongata P. fortunei exhibited the highest frequency of axillary shoot proliferation following by P. tomentosa P. fortunei. The results are discussed with the perspective of applying an improved protocol for in vitro seed germination and plantlet formation in several economically valuable Paulownia genotypes.

  20. In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia

    Directory of Open Access Journals (Sweden)

    Ummi Nur Ain Abdul Razak

    2014-02-01

    Full Text Available Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP and Kinetin (Kn for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA, Indole-3-butyric acid (IBA and 1-Naphthaleneacetic acid (NAA for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants was observed on a Murashige and Skoog (MS medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in plant soil with survival rate of 83.3 %.

  1. Optimization of a protocol for the micropropagation of pineapple

    Directory of Open Access Journals (Sweden)

    ALMEIDA WELITON ANTONIO BASTOS DE

    2002-01-01

    Full Text Available The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L. Merrill cv. Pérola. Changes in benzylaminopurine (BAP concentration, type of medium (liquid or solidified and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%, for three minutes, followed by calcium hypochlorite (2%, for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1, 27 ± 2ºC. After eight weeks, cultures were subcultured for multiplication in MS medium. The following treatments were tested: liquid x solidified medium with different BAP concentrations (0.0, 1.5 or 3.0 mg L-1, and the longitudinal cut, or not, of the shoot bud used as explant. The results showed that liquid medium supplemented with BAP at 1.5 mg L-1, associated with the longitudinal sectioning of the shoot bud used as explant presented the best results, maximizing shoot proliferation. On average, the best treatment would allow for an estimated production of 161,080 plantlets by the micropropagation of the axillary buds of one plant with eight slips and ten buds/slips, within a period of eight months.

  2. Review by a local medical research ethics committee of the conduct of approved research projects, by examination of patients' case notes, consent forms, and research records and by interview.

    OpenAIRE

    Smith, T.; Moore, E. J.; Tunstall-Pedoe, H.

    1997-01-01

    OBJECTIVE: To monitor the conduct of medical research projects that have already been approved by the local medical research ethics committee. DESIGN: Follow up study of ethically approved studies (randomly selected from all the studies approved in the previous year) by examination of patients' case notes, consent forms, and research records and by interview of the researchers at their workplace. SETTING: Tayside, Scotland (mixed rural and urban population). SUBJECTS: 30 research projects app...

  3. Editorial note

    DEFF Research Database (Denmark)

    Gani, Rafiqul; Hrymak, A.; Lee, J.

    2009-01-01

    industrial and educational applications. These articles will highlight theory, models, algorithms and applications with respect to value preservation and/or value creation or growth within the chemical product supply chain. To highlight and motivate research in the emerging challenges in PSE, we plan...... components of PSE—modeling, numerical analysis, optimization, systems and control theory, computer science, and, management science will be highlighted through the published articles (full-length papers, perspective papers, review papers, short notes and letters to the editor). They will cover...... and intelligent systems, integrated approaches to design, control and data analysis, systematic techniques for managing complexity, etc.), PSE emerging domains (product-process design, enterprise-wide optimization, energy and sustainability, biological engineering, pharmaceutical engineering, etc.) and novel...

  4. Yellow-cedar in vitro clonal production and evaluation of propagules for reforestation. FRDA research memo No. 211

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    Technical note describing a project that was part of an ongoing effort to develop micropropagation techniques on an operation scale and to investigate their possible use in producing genetically improved stock for planting. The project developed a commercially viable process that uses organogenic micropropagation to produce yellow-cedar stock for operational reforestation; evaluated the techniques for developing genetically improved clones; and established demonstration plots of mircopropagules.

  5. A Research Note on Time With Children in Different- and Same-Sex Two-Parent Families.

    Science.gov (United States)

    Prickett, Kate C; Martin-Storey, Alexa; Crosnoe, Robert

    2015-06-01

    Public debate on same-sex marriage often focuses on the disadvantages that children raised by same-sex couples may face. On one hand, little evidence suggests any difference in the outcomes of children raised by same-sex parents and different-sex parents. On the other hand, most studies are limited by problems of sample selection and size, and few directly measure the parenting practices thought to influence child development. This research note demonstrates how the 2003-2013 American Time Use Survey (n=44,188) may help to address these limitations. Two-tier Cragg's Tobit alternative models estimated the amount of time that parents in different-sex and same-sex couples engaged in child-focused time. Women in same-sex couples were more likely than either women or men in different-sex couples to spend such time with children. Overall, women (regardless of the gender of their partners) and men coupled with other men spent significantly more time with children than men coupled with women, conditional on spending any child-focused time. These results support prior research that different-sex couples do not invest in children at appreciably different levels than same-sex couples. We highlight the potential for existing nationally representative data sets to provide preliminary insights into the developmental experiences of children in nontraditional families.

  6. Note by Note: a New Revolution in Cooking

    OpenAIRE

    Burke, Roisin; Danaher, Pauline

    2016-01-01

    Note by note cooking is an application of Molecular Gastronomy. It was first proposed by French Physical Chemist and Molecular Gastronomy Co-founder, Hervé This. Note by Note dishes are being created as part of Ph.D. research in the Dublin Institute of Technology, Cathal Brugha Street.

  7. Maintaining knowledge, training and infrastructure for research and development in nuclear safety. A note by the International Nuclear Safety Advisory Group

    International Nuclear Information System (INIS)

    International Nuclear Safety Advisory Group

    2001-01-01

    The purpose of this INSAG Note is to emphasize the importance of maintaining capabilities for nuclear research and education, especially with regard to safety aspects, so that nuclear safety may be maintained in IAEA Member States, and to alert Member States to the potential for significant harm if the infrastructure for research, development and education is not maintained

  8. Zebra Mussel Research Technical Notes. Impacts of Zebra Mussel Infestations on Water Quality. Section 1 - Environmental Testing

    National Research Council Canada - National Science Library

    Ashby, Steven

    1998-01-01

    ..., and sediment quality. The purpose of this technical note describes potential changes in water quality as a result of zebra mussel infestations in aquatic systems, based on a review of the literature...

  9. Editorial note

    Directory of Open Access Journals (Sweden)

    Tore Ahlbäck

    2009-01-01

    Full Text Available Editorial note of the Scripta Instituti Donneriani Aboensis, vol. 21, Postmodern Spirituality, based on papers read at the symposium on Postmodern Spirituality held at Åbo, Finland, on 11–13 June 2008.

  10. Project Notes

    Science.gov (United States)

    School Science Review, 1978

    1978-01-01

    Presents sixteen project notes developed by pupils of Chipping Norton School and Bristol Grammar School, in the United Kingdom. These Projects include eight biology A-level projects and eight Chemistry A-level projects. (HM)

  11. Micropropagation of onion (Allium cepa L.) from immature inflorescences.

    Science.gov (United States)

    Marinangeli, Pablo

    2013-01-01

    In vitro plant production by direct organogenesis from immature flower heads is an ideal approach for clonal propagation of onions (Allium cepa L.). This technique ensures genetic stability, high propagation rate, and maintains donor plant of explants with an advantage over other means of in vitro regeneration. Onion micropropagation is usually applied in breeding programs, maintenance, and multiplication of cytoplasmic-male sterile lines for hybrid production, germplasm conservation, and as a tool for the application of other biotechnologies. For in vitro culture, mature onion bulbs are induced to reproductive phase by vernalization and forced to inflorescence initiation. Immature umbels are dissected from bulbs or cut directly when they appear from the pseudostem among the leaves. Disinfected inflorescences are cultivated in BDS basal medium supplemented with 30 g/L sucrose, 0.1 mg/L naphthalene acetic acid, 1 mg/L N (6)-benzyladenine, and 8 g/L agar, pH 5.5, under 16 h photoperiod white fluorescent light (PPD: 50-70 μmol/m(2)s) for 35 days. The regenerated shoot clumps are divided and subculture under the same conditions. For bulbification phase, the individual shoots are cultured in BDS basal medium containing 90 g/L sucrose, without plant growth regulators, pH 5.5, under 16 h photoperiod. Microbulbs can be directly cultivated ex vitro without acclimation.

  12. Micropropagation of chokeberry by in vitro axillary shoot proliferation.

    Science.gov (United States)

    Litwińczuk, Wojciech

    2013-01-01

    The black chokeberry-aronia (Aronia melanocarpa Elliot) is a shrub native to North America although nowadays well known in Eastern Europe. The fruits are regarded as the richest source of antioxidant phytonutrients among fruit crops and vegetables. Chokeberries can be easily propagated by seeds but this method is not recommended. Micropropagation is far more efficient than other conventional cloning methods like layering or softwood cuttings. Aronia clones are propagated in vitro through four- or three-stage method based on subculturing of shoot explants. The double diluted MS or full strength MS medium with elevated 50% Ca(2+) and Mg(2+) content are used in the initiation and proliferation chokeberry in vitro cultures, respectively. They are supplemented with 0.5-1.0 mg LBA, and 0.05 mg LIBA. The double-phase medium is recommended in the last passage before shoot rooting. The regenerated shoots could be rooted both in vitro on double diluted MS with 0.05 mg L(-1) IBA or in vivo in peat and perlite substrate and subsequently grown in the greenhouse.

  13. Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments.

    Science.gov (United States)

    Silveira, S S; Cordeiro-Silva, R; Degenhardt-Goldbach, J; Quoirin, M

    2016-05-03

    Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium, at any concentration. Polyvinylpyrrolidone, activated charcoal, cysteine, ascorbic acid or citric acid were added to the culture medium to avoid oxidation. After 30 days of culture, polyvinylpirrolidone and ascorbic acid gave better results, eliminating oxidation in most explants. For shoot multiplication, benzylaminopurine was used in concentrations of 4.4 and 8.8 µM in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively, after 90 days. Indole-3-butyric acid and α-naphthalene acetic acid were used to induce root formation, reaching a maximum rooting rate of 24% with 20µM α-naphthalene acetic acid. For acclimatization. the rooted plants were transferred to Plantmax® substrate and cultured in a greenhouse, reaching 79% of survival after 30 days and 60% after one year.

  14. Phenotypic aberrations during micropropagation of Soymida febrifuga (Roxb. Adr. Juss

    Directory of Open Access Journals (Sweden)

    Kishore Kumar CHIRUVELLA

    2014-03-01

    Full Text Available Like most of the medicinal plants Soymida febrifuga (Meliaceae possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protocols for in vitro mass propagation has been established from field grown and aseptic seedlings explants. Strikingly, we observed aberrant structures such as vitrified shoots, faciated shoots, albino shoots as well shoot necrosis during its micropropagation. These phenotypic maladies were observed during organogenesis and rooting. Compared to other abnormalities, shoot necrosis nonetheless was frequent and pronounced leading to plant death. Shoots when subjected to rooting also displayed necrosis which was controlled by transferring to MS medium containing various concentrations and combinations of calcium levels, activated charcoal, glucose, fructose and auxins. Microshoots initiated roots on half strength MS medium with IBA and IAA individually or in combination within two weeks. MS half strength solid medium supplemented with CAN (556 mg l–1, CAP (1.0 mg l–1, IAA (2.0 mg l–1 and IBA (2.0 mg l–1 in combination was found to be more efficient in showing high frequency (95% of root regeneration. Rooted plantlets were successfully hardened and 70-85% of regenerated plants were successfully acclimatized to natural environment. In vitro derived plantlets were morphologically similar to in vivo plants.

  15. Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress.

    Science.gov (United States)

    Saher, Shady; Piqueras, Abel; Hellin, Eladio; Olmos, Enrique

    2004-01-01

    The physiology of hyperhydricity in relation to oxidative stress, mineral nutrients, antioxidant enzymes and ethylene has been studied in three micropropagated carnation cultivars under experimentally induced hyperhydricity. A marked increase in Fe content in comparison with normal tissues was observed in the hyperhydric tissues from the three cultivars. The levels of ethylene, solute leakage and malondialdehyde content were also significantly higher in the hyperhydric tissues. In relation to the time course of H(2)O(2) production measured by fluorescence quenching, a similar trend could be observed for the three cultivars, with a clear increase in the generation of hydrogen peroxide in hyperhydric tissues. The activities of all the antioxidative enzymes studied, except lipoxygenase, were higher in the hyperhydric shoots. Phenylalanine ammonia-lyase (PAL) showed a significant decrease in activity in the hyperhydric tissues in comparison with the controls for the three cultivars. Soluble guaiacol peroxidase had a strong increase in activity in hyperhydric shoots of the three cultivars. These results provide, for the first time, direct evidence of H(2)O(2) generation in hyperhydric tissues, characterize the response of the antioxidant system to an oxidative stress during hyperhydricity in carnation leaves and point to the accumulation of toxic forms of oxygen as the inducer of some of the abnormalities observed.

  16. Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

    Directory of Open Access Journals (Sweden)

    Daniele Brandstetter Rodrigues

    2016-10-01

    Full Text Available An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated- various concentrations of 6-benzylaminopurine (BAP (0.0, 1.0, 2.0, and 3.0 mg L-1 and substrates (agar, vermiculite, and coconut fiber added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA (0.0, 0.5, 1.0, and 1.5 mg L-1 were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L-1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture.

  17. Lagos Notes and Records

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... Lagos Notes and Records is an annual, interdisciplinary journal of the humanities. ... Insuring the Nation: Europeans and the Emergence of Modern Insurance Business in Colonial Nigeria ...

  18. Review by a local medical research ethics committee of the conduct of approved research projects, by examination of patients' case notes, consent forms, and research records and by interview.

    Science.gov (United States)

    Smith, T; Moore, E J; Tunstall-Pedoe, H

    1997-05-31

    To monitor the conduct of medical research projects that have already been approved by the local medical research ethics committee. Follow up study of ethically approved studies (randomly selected from all the studies approved in the previous year) by examination of patients' case notes, consent forms, and research records and by interview of the researchers at their workplace. Tayside, Scotland (mixed rural and urban population). 30 research projects approved by Tayside local medical research ethics committee. Adherence to the agreed protocol, particularly for recruitment (obtaining and recording informed consent) and for specific requirements of the ethics committee, including notification of changes to the protocol and of adverse events. In one project only oral consent had been obtained, and in a quarter of the studies one or more consent forms were incorrectly completed. Inadequate filing of case notes in five studies and of consent forms in six made them unavailable for scrutiny. Adverse events were reported, but there was a general failure to report the abandoning or non-starting of projects in two studies the investigators failed to notify a change in the responsible researcher. Monitoring of medical research by local medical research ethics committees promotes and preserves ethical standards, protects subjects and researchers, discourages fraud, and has the support of investigators. We recommend that 10% of projects should undergo on-site review, with all others monitored by questionnaire. This would require about six person hours of time and a salary bill of 120 pounds per study monitored.

  19. Micropropagation of Pithecellobium dulce (Roxb.) Benth-a multipurpose leguminous tree and assessment of genetic fidelity of micropropagated plants using molecular markers.

    Science.gov (United States)

    Goyal, Pooja; Kachhwaha, Sumita; Kothari, S L

    2012-04-01

    An efficient and reproducible protocol has been developed for in vitro propagation of Pithecellobium dulce (Roxb.) Benth (a multipurpose leguminous tree) from field grown nodal segments (axillary bud). Shoot bud induction occurred from nodal explants of 15-years-old tree on Murashige and Skoog (MS) basal medium supplemented with 4.4 μM 6-benzyladenine (BA) and multiplication was achieved on MS medium supplemented with 4.4 μM BA + 0.73 μM phenylacetic acid (PAA) i.e. up to 7 shoot buds in the period of 5-6 weeks. Addition of adenine sulphate (AdS) to this medium further enhanced the number of shoot buds up to 10. Proliferating shoot cultures were established by repeatedly subculturing primary culture on fresh medium (MS + 4.4 μM BA + 0.73 μM PAA) after every 25 days. In vitro rooting was achieved on MS medium supplemented with 2.46 μM Indole-3-butyric acid (IBA) + 41.63 μM activated charcoal (AC). The micropropagated shoots with well developed roots were acclimatized in green house in pots containing sand, soil and manure (1:1:1). Genetic stability of micropropagated clones was evaluated using Random amplified polymorphic DNA (RAPD) and Inter simple sequence repeat (ISSR) markers. The amplification products were monomorphic in micropropagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic uniformity of micropropagated plants. This is the first report of an efficient protocol for regeneration of P. dulce through organogenesis, which can be used for further genetic transformation and pharmaceutical purposes.

  20. SAFETY NOTES

    CERN Document Server

    TIS Secretariat

    2001-01-01

    Please note that the revisions of safety notes no 3 (NS 3 Rev. 2) and no 24 (NS 24 REV.) entitled respectively 'FIRE PREVENTION FOR ENCLOSED SPACES IN LARGE HALLS' and 'REMOVING UNBURIED ELV AND LVA ELECTRIC CONDUITS' are available on the web at the following urls: http://edmsoraweb.cern.ch:8001/cedar/doc.download?document_id=322811&version=1&filename=version_francaise.pdf http://edmsoraweb.cern.ch:8001/cedar/doc.download?document_id=322861&version=2&filename=version_francaise.pdf Paper copies can also be obtained from the TIS Divisional Secretariat, email tis.secretariat@cern.ch

  1. Effect of arbuscular mycorrhiza on the growth and development of micropropagated Annona cherimola plants

    Directory of Open Access Journals (Sweden)

    Concepcion Azcón-Aguilar

    1994-05-01

    Full Text Available Annona cherimola Mill., cherimoya, is a tropical plantation crop of interest in fruit culture. Micropropagation techniques have been developed due to the need to increase productivity through clonal selection. Because of the mycorrhizal dependence exhibited by this crop for optimal growth and the recognized role of mycorrhiza establishment for the survival and development of most of the plants produced in vitro, the effect of mycorrhiza inoculation on the development of micropropagated plants of Annona cherimola was investigated. Mycorrhizal inoculation was assayed at two different stages of the micropropagation process: (i immediately after the in vitro phase, before starting the acclimatization period, and (ii after the acclimatization phase, before starting the post-acclimatization period under greenhouse conditions. Plantlet survival was about 50 % after the acclimatization period. Plant growth and development profited remarkably from mycorrhiza establishment. Most of the arbuscular mycorrhizal fungi (AMF assayed greatly increased shoot and root biomass and leaf area. Micropropagated Annona plants seem to be more dependent on mycorrhiza formation for optimal growth than plants derived from seeds. The greatest effects of AMF on plant growth were observed when they were introduced after the acclimatization period.

  2. Use of plastic films for weed control during field establishment of micropropagated hardwoods

    Science.gov (United States)

    J. W. Van Sambeek; John E. Preece; Carl A. Huetteman; Paul L. Roth

    1995-01-01

    This study compares the use of plastic films to conventional methods for establishing hardwoods on a recently cultivated old field site using 1-year-old micropropagated plantlets of white ash (Fraxinus americana L.) and silver maple (Acer saccharinum L.). After one growing season in the field, height of plantlets with all weed...

  3. How does exogenously applied cytokinin type affect growth and endogenous cytokinins in micropropagated Merwilla plumbea?

    Czech Academy of Sciences Publication Activity Database

    Aremu, A.O.; Plačková, Lenka; Bairu, M. W.; Novák, Ondřej; Plíhalová, Lucie; Doležal, Karel; Finnie, J. F.; van Staden, J.

    2014-01-01

    Roč. 118, č. 2 (2014), s. 245-256 ISSN 0167-6857 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : Conservation * Medicinal plant * Micropropagation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.125, year: 2014

  4. Micropropagation of Alstroemeria in liquid medium using slow release of medium components

    NARCIS (Netherlands)

    Klerk, de G.J.M.; Brugge, ter J.

    2010-01-01

    Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous

  5. Mass micropropagation of pineapple tissue culture using bioreactor technology

    International Nuclear Information System (INIS)

    Irwan Syafri; Amir Hamzah Harun; Rusli Ibrahim

    2005-01-01

    Pineapple (ananas comosus) is the most important fruit in terms of revenue earner in this country. The export of the canned pineapple is about 2 million standard cases annually valued at RM 60 million, while the export of fresh pineapple is about 40,000 tonnes worth about RM 10 million. The industry for canning is however, an ailing industry with production on the decline since the 70s. Scaling up the pineapple propagation using in vitro methods seems to be possible solutions for the lack of planting material. Temporary immersion system (TIS) has been described by Teisson and Alvard (1995) for plant tissue culture propagation. This system, also known as RITA, has been successfully used with embryogenic tissues of banana (Alvard et al 1993), coffee (Berthouly 1991), rubber (Etienne et al 1993) and sugarcane (Lorenzo et al 1998). In this study, the system has been set up with a potential capacity of 3 manifolds with 10 RITA each, to multiply meristem explants at different immersion periods. The system was compared with the conventional micropropagation system on solid medium. Both systems were treated with MS media containing 2.5 mg/l BAP and 0.1 NAA. In TIS the shoots were able to multiplied faster in comparison with solid media. The multiplication rates were increased up to 1:3 to 1:5 compared to normal propagation on solid media. The results show that TIS not only increase the propagation rates of pineapple but could also be adapted to reduce implementation costs to establish low-cost propagation systems. (Author)

  6. Tissue culture and micropropagation for forest biomass production

    Energy Technology Data Exchange (ETDEWEB)

    Mason, E.; Maine, F.W.

    1984-09-01

    An increase in forest production will be necessary in the future when wood becomes a major renewable source of energy and chemicals along with its traditional role of fibre source. This increase could eventually by achieved be proper selection and breeding of trees. Clonal forestry by vegetative propagation of cuttings is becoming a viable alternative to a seedling-based forestry with many advantages, and cutting could be used to quickly propagate large numbers of clones of control-pollinated seedlings. Most forest trees are propagated sexually and seed orchards were started in the US and Canada in the last 40-50 years for breeding purposes. Forests could ultimately be established with improved seedlings instead of from seed with unknown genetic potential, or by natural regeneration. Micropropagation is the term used to refer to the propagation of plants raised by tissue culture methods rather than from seeds or cuttings. Many clonal plantlets could be regenerated asexually in the laboratory and eventually transplanted to permanent sites. In addition the technology could be developed to produce new variants from somatic cells. Tissue culture is a technique which may be useful for plant propagation where conventional methods are inadequate or unsuitable. However, traditional studies of field planting observed over long periods of time would still be necessary. This document has the object of informing those who may wish to know more about these techniques in relation to practical application, and require a general overview rather than experimental details, which are given in an annotated bilbiography. 274 refs., 2 figs., 1 tab.

  7. Technical Note

    African Journals Online (AJOL)

    Administratör

    In this report on four patients, we did not use any of these techniques. The existence and the site of the fistulas was clearly demonstrated using basic but important preoperative detailed assessment and two intraoperative findings. The preoperative referral note that indicated the site of technical difficulty during the previous ...

  8. Please note

    CERN Multimedia

    2006-01-01

    Members of the personnel are invited to take note that only parcels corresponding to official orders or contracts will be handled at CERN. Individuals are not authorised to have private merchandise delivered to them at CERN and private deliveries will not be accepted by the Goods Reception services. Thank you for your understanding. (Version française la semaine prochaine.)

  9. Editor's Note

    African Journals Online (AJOL)

    On another note: the editor and the editorial team acknowledge the financial support of the Carnegie Corporation of New York through the University of Ghana Building A New Generation of Academics in Africa (BANGA-Africa) Project. We also use this platform to express our gratitude for the support of various stakeholders, ...

  10. Genetic characterization, micropropagation, and potential use for arsenic phytoremediation of Dittrichia viscosa (L.) Greuter.

    Science.gov (United States)

    Guarino, Francesco; Conte, Barbara; Improta, Giovanni; Sciarrillo, Rosaria; Castiglione, Stefano; Cicatelli, Angela; Guarino, Carmine

    2018-02-01

    In the last decade, many scientists have focused their attention on the search for new plant species that can offer improved capacities to reclaim polluted soils and waters via phytoremediation. In this study, seed batches from three natural populations of Dittrichia viscosa, harvested in rural, urban, and industrial areas of central and southern Italy, were used to: (i) evaluate the genetic and morphological diversity of the populations; (ii) develop an efficient protocol for in-vitro propagation from seedling microcuttings; (iii) achieve optimal acclimatization of micropropagated plants to greenhouse conditions; (iv) test the response to arsenic (As) soil contamination of micropropagated plants. The genetic biodiversity study, based on Random Amplification of Polymorphic DNA (RAPD), as well as the morphometric analysis of 20 seedlings from each population revealed some degree of differentiation among populations. Based on these data, the most biodiverse plants from the three populations (10 lines each) were clonally multiplied by micropropagation using microcuttings of in-vitro grown seedlings. Three culture media were tested and Mureshige and Skoog medium was chosen for both seedling growth and micropropagation. The micropropagated plants responded well to greenhouse conditions and over 95% survived the acclimatization phase. Four clones were tested for their capacity to grow on soil spiked with NaAsO 2 and to absorb and accumulate the metalloid. All clones tolerated up to 1.0mg As. At the end of the trial (five weeks), As was detectable only in leaves of As-treated plants and concentration varied significantly among clones. The amount of As present in plants (leaves) corresponded to ca. 0.10-1.7% of the amount supplied. However, As was no longer detectable in soil suggesting that the metalloid was taken up, translocated and probably phytovolatilized. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles.

    Science.gov (United States)

    Puente-Garza, César A; Gutiérrez-Mora, Antonia; García-Lara, Silverio

    2015-01-01

    Maguey, Agave salmiana, is an important plant for the "pulque" beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX) activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild-type (WT) plants, in in vitro (IN) plants, and ex vitro acclimated plants (EN). The results showed that IN plants have a 50% lower soluble sugar content compared to WT, and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN) and regenerated (EN) plants compared to WT. The total saponin content in IN, and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = -0.927; p = 0.003) was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to WT plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants.

  12. Micropropagation of Agave salmiana: Means to production of antioxidant and bioactive principles.

    Directory of Open Access Journals (Sweden)

    César Armando Puente-Garza

    2015-11-01

    Full Text Available Maguey, Agave salmiana, is an important plant for the pulque beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild type (WT plants, in in vitro (IN plants, and ex vitro acclimated plants (EN. The results showed that IN plants have a 50% lower soluble sugar content compared to WT and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN and regenerated (EN plants compared to WT. The total saponin content in IN and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = -0.927; p = 0.003 was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to wild plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants.

  13. MixedNotes

    DEFF Research Database (Denmark)

    Jokela, Tero; Lucero, Andrés

    2014-01-01

    Affinity Diagramming is a technique to organize and make sense of qualitative data. It is commonly used in Contextual Design and HCI research. However, preparing notes for and building an Affinity Diagram remains a laborious process, with a wide variety of different approaches and practices....... In this paper, we present MixedNotes, a novel technique to prepare physical paper notes for Affinity Diagramming, and a software tool to support this technique. The technique has been tested with large real-life Affinity Diagrams with overall positive results....

  14. Application note :

    Energy Technology Data Exchange (ETDEWEB)

    Russo, Thomas V.

    2013-08-01

    The development of the XyceTM Parallel Electronic Simulator has focused entirely on the creation of a fast, scalable simulation tool, and has not included any schematic capture or data visualization tools. This application note will describe how to use the open source schematic capture tool gschem and its associated netlist creation tool gnetlist to create basic circuit designs for Xyce, and how to access advanced features of Xyce that are not directly supported by either gschem or gnetlist.

  15. CLONAL MICROPROPAGATION OF POTATO VARIETIES BY WESTERN SIBERIA SELECTION– THE NEW FEATURES

    Directory of Open Access Journals (Sweden)

    E.P. Miakisheva

    2015-04-01

    Full Text Available The article is sanctified urgent need to use methods of modern biotechnology in primary seed farming of potatoes in the territory of the Russian Federation. Lack of required amount of good quality planting material is a major factor limiting stable high yield. The crop of potatoes, annually produced in Russia, significantly below global, and does not meet genetic capabilities of the species. Many viral infections tend to accumulate in the tubers, reducing their quality and keeping quality during storage. For the first time peculiarities of clonal micropropagation and in vitro culture of early-maturing potato varieties Lubava Russian breeding (agricultural research Institute of the city of Kemerovo, the Kemerovo region of the Russian Federation. At the stage of introduction to the culture of the parameters, providing pure cultures using lizoformin as sterilizing compounds. At the stage of actually breeding to obtain plants-regenerants of a certain morphological structure was studied the influence of certain components of the nutrient medium. The influence of agar, sucrose and vitamins such morphological indicators of development of regenerated plants as number of internodes and plant height. In the rooting stage the effect of different of growth regulators auxin nature: α-naphthyloxy acid, β-indoleacetic acid and β-indolebutyric acid, in concentrations of 0.1-5 μm. Selected concentrations of these compounds, the introduction of which in the nutrient medium provided the maximum indices of rhisogenesis: the number and length of roots. At the stage of adaptation to non-sterile growing conditions ex vitro the efficiency of the hydroponic plants, filled with a liquid nutrient solution. A successful stage adaptation has characterizability 100% survival rate of plants and increase the morphological characteristics of regenerants.

  16. Effect of light in the micropropagation of clones series I.S. - Prunus spp. rootstocks

    International Nuclear Information System (INIS)

    De Rossi, A.; Rufato, L.; Fiaschi, G.; Morini, S.; Loreti, F.

    2004-01-01

    The principal problem in the micropropagation of clones from the I.S. Series is the difficulty of internodes elongation. This series of clones was developed in the “Dipartimento de Coltivazione e Difesa delle Specie Legnose G. Scaramuzzi” which belongs to the “Università Degli Studi di Pisa”. The objective of the research was to improve the proliferate and elongation response of the clones explants from the series I.S. 5/18, 5/23 and 5/14 with the application of different light quality, quantity and intensity. Thys, vegetative apices with at least 1 cm of length were used. These explants were submitted to 5 treatments: 1) control (fluorescent light Philips TLD 18W/33), 2) incandescent light, 3) reduction of light intensity (30% less than the control), 4) photoperiod of 16/8 hours and 5) red light. The experimental design used was completely randomized, with 15 replications per treatment. The results were submitted to a comparison of means through the Duncan Test. Sixty days after the on set of the experiment, the average number of developed budding, the length of the internodes, the degree of vitrification and the green color intensity of the explants were evaluated. The treatment using incandescent light has increased the length on the internodes to the clone I.S. 5/18, and also has reduced the vitrification and the intensity of green color on the 3 clones. The red light has reduced the proliferation rate of the clones from the I. S. series. (author) [pt

  17. Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse.

    Science.gov (United States)

    Zakavi, Maryam; Askari, Hossein; Irvani, Neda

    2016-10-01

    The present study concentrated on introducing a micropropagation protocol for a drought resistant genotype from Pyrus boissieriana , which is the second most naturally widespread pear species in Iran with proper physiological and medicinal properties. Proliferating microshoot cultures were obtained by placing nodal segments on MS medium supplemented with BAP and IBA or NAA. The highest number of shoots (27 shoots per explant) were obtained with 1.5 mg l -1 BAP and 0.05 mg l -1 IBA, but this combination did not produce shoots of desirable length (>1.7 cm). Combination of 1.75 mg l -1 BAP and 0.07 mg l -1 IBA was the best for the shoot multiplication in P. boissieriana with a sufficient number of shoot production (22.33 shoots per explant) and relatively more appropriate shoot length. The larger and greenish leaves were obtained when PG was added to the best multiplication treatment. Microshoot elongation was carried out in 1/2 and 1/4 MS medium containing 50-100 mg l -1 PG with different concentrations of IBA or NAA at intervals of 30-60 days. Significant increase in shoot length was detected after 45-60 days of culture in the presence of PG. The highest shoot length (8 cm) was recorded on 1/2 MS medium supplemented with 0.5 mg l -1 IBA and 100 mg l -1 PG. GA 3 negatively affected number and length of shoots and generally caused generation of red leaves. The highest percentage of root induction (100%) and root length (9 cm) were obtained on 1/6 strength MS medium supplemented with 0.005 mg l -1 IBA. All plantlets were hardened when transferred to ex vitro conditions through a period of 25-30 days. The results suggest axillary shoot proliferation of P. boissieriana could successfully be employed for propagation of candidate drought resistant seedling.

  18. Editors' note

    Science.gov (United States)

    Denker, Carsten; Feller, Alex; Schmidt, Wolfgang; von der Lühe, Oskar

    2012-11-01

    This topical issue of Astronomische Nachrichten/Astronomical Notes is a collection of reference articles covering the GREGOR solar telescope, its science capabilities, its subsystems, and its dedicated suite of instruments for high-resolution observations of the Sun. Because ground-based telescopes have life spans of several decades, it is only natural that they continuously reinvent themselves. Literally, the GREGOR telescope builds on the foundations of the venerable Gregory-Coudé Telescope (GCT) at Observatorio del Teide, Tenerife, Spain. Acknowledging the fact that new discoveries in observational solar physics are driven by larger apertures to collect more photons and to scrutinize the Sun in finer detail, the GCT was decommissioned and the building was made available to the GREGOR project.

  19. Determine the effective in vitro micro-propagation methods for Tunceli garlic (Allium tuncelianum (Kollman))

    International Nuclear Information System (INIS)

    2011-01-01

    This research was conducted at University of Ankara, Faculty of Agriculture, Department of Horticulture and Turkish Atomic Energy Authority Saraykoey Nuclear Research and Training Center, Plant Breeding Lab's at Agriculture Division within the period of 2004-2006. The scope of this research is to determine the effective in vitro micro-propagation methods as shoot and root culture for Tunceli garlic. In this research to determine the best combinations of growth regulators for shoot and root cultures, 2.4-D and NAA (0, 1.0, 2.0 mgl - 1) were used as auxin and BA (0, 0.1, 1.0 mgl - 1) as cytokinin. No shoots obtained from the leave and root tip culture experiment. Only callus formation was seen in 0.1 mgl - 1 BA+1.0 mgl - 1 NAA combination. Both auxins and cytokinin failed to induce shoot proliferation in Tunceli garlic via shoot culture experiment. Only one shoot was obtained from the 0.05 mg/l BA+0.1 mgl - 1 IAA, 0.1 mgl - 1 BA and 0.1 mgl - 1 BA + 0.1 mgl - 1 NAA, 0.05 mgl - 1 BA+0.5 mgl - 1 IAA and 0.05 mgl - 1 BA combinations. Proliferation frequency increased with lower concentration of IAA with 0.05 mg/l BA. In vitro bulblet formation of garlic shoots started from second subcultures. When the subculture number was increased, also bulbing ratio increased too. Bulb formation ratio was 100% in medium without auxin and 0.1 mgl-NAA+0.1 mgl - 1 BA combination. Shoots do not formed bulblet planted again on medium without auxin or with 0.1 mgl - 1 NAA. Although shoots were started rooting (37%) in without auxin and 40% in 0.1 mgl - 1 NAA from fourth subculture in bulblet formation medium, rooting ratio was found as low level. When unrooted shoots transferred to the medium without auxin and with NAA medium, rooting ratio increased. The highest rooting ratio was 33% in 2 mgl - 1 NAA, and 17% in 0.5 mgl - 1 NAA. When rooted and unrooted garlic bulblets transferred pots, plants died after produced 2-3 leaves but small bulblets were obtained from main bulblets. This is an

  20. Automatic micropropagation of plants--the vision-system: graph rewriting as pattern recognition

    Science.gov (United States)

    Schwanke, Joerg; Megnet, Roland; Jensch, Peter F.

    1993-03-01

    The automation of plant-micropropagation is necessary to produce high amounts of biomass. Plants have to be dissected on particular cutting-points. A vision-system is needed for the recognition of the cutting-points on the plants. With this background, this contribution is directed to the underlying formalism to determine cutting-points on abstract-plant models. We show the usefulness of pattern recognition by graph-rewriting along with some examples in this context.

  1. Micropropagation in vitro de la variété locale « Aloga » du bananier ...

    African Journals Online (AJOL)

    Leur transfert en plein champ a permis d'avoir des plantes avec une taille moyenne d'environ 20 cm, un diamètre à la base de la tige avoisinant 10 cm et un nombre de feuilles vertes de l'ordre de 6 après 3 à 4 mois de culture en plein champ. Cette étude ouvre la voie au développement de la micropropagation du plantain ...

  2. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    OpenAIRE

    Silva,Aurilena de Aviz; Carvalho,Almy Junior Cordeiro de; Freitas,Flávia Paiva de; Pessanha,Patrícia Gomes de Oliveira; Santos,Paulo Cesar dos; Silva,Mírian Peixoto Soares da; Vasconcelos,Tábatha de Souza; Olivares,Fábio Lopes

    2016-01-01

    ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholder...

  3. Plant abiotic stress tolerance analysis in cauliflower using a curd micropropagation system

    OpenAIRE

    Rihan, HZ; Al-Issawi, M; Al-Shamari, M; Elmahrouk, M; Fuller, MP

    2015-01-01

    © 2015 ISHS. An effective protocol for cauliflower micropropagation was optimised and developed which enabled the production of tens of thousands of cauliflower microshoots from one cauliflower curd. The large number of microshoots that can be produced per culture unit facilitates the use of this protocol to analyse both the physiological and molecular components of abiotic stress tolerance. The protocol was used for cauliflower cold tolerance analysis and it was demonstrated that low tempera...

  4. Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers.

    Science.gov (United States)

    Akdemir, Hülya; Suzerer, Veysel; Tilkat, Engin; Onay, Ahmet; Çiftçi, Yelda Ozden

    2016-12-01

    Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in "reasonable polymorphism" among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.

  5. Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.

    Science.gov (United States)

    Vidal, Á M; Vieira, L J; Ferreira, C F; Souza, F V D; Souza, A S; Ledo, C A S

    2015-07-14

    Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard's index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.

  6. Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator.

    Science.gov (United States)

    Kalinina, Anna; Brown, Daniel C W

    2007-07-01

    A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 peach, commonly used for Prunus virus indexing. The micropropagation cycle based on proliferation of vegetative tissues includes establishment of tissue culture through introduction of shoot meristems in vitro, shoot proliferation, root induction and plant acclimatization steps and can be completed in 5 months. A meristem sterilization protocol minimized bacterial and fungal contamination. Multiple shoot formation in ornamental Prunus was obtained through the use of 1 mg l(-1) 6-benzyladenine. For GF305 peach, alteration in the sugar composition, fructose instead of sucrose, and addition of 1 mg l(-1 )ferulic acid had a significant impact on the shoot proliferation rate and maintenance of long-term in vitro culture. Rooting and plant acclimatization conditions were improved using a two-step protocol with a 4-day root induction in indole-3-butiric acid (IBA)-containing media with consequent 3-week root elongation in IBA-free media. One-month incubation of rooted shoots in a vermiculite-based medium resulted in additional shoot and root growth and provided better acclimatization and plant recovery. The micropropagation approach can be used for maintenance of the clonal properties for Prunus spp. as well as a protocol to support meristem therapy against viral infection.

  7. Editorial Note

    Science.gov (United States)

    van der Meer, F.; Ommen Kloeke, E.

    2015-07-01

    With this editorial note we would like to update you on the performance of the International Journal of Applied Earth Observation and Geoinformation (JAG) and inform you about changes that have been made to the composition of the editorial team. Our Journal publishes original papers that apply earth observation data for the management of natural resources and the environment. Environmental issues include biodiversity, land degradation, industrial pollution and natural hazards such as earthquakes, floods and landslides. As such the scope is broad and ranges from conceptual and more fundamental work on earth observation and geospatial sciences to the more problem-solving type of work. When I took over the role of Editor-in-Chief in 2012, I together with the Publisher set myself the mission to position JAG in the top-3 of the remote sensing and GIS journals. To do so we strived at attracting high quality and high impact papers to the journal and to reduce the review turnover time to make JAG a more attractive medium for publications. What has been achieved? Have we reached our ambitions? We can say that: The submissions have increased over the years with over 23% for the last 12 months. Naturally not all may lead to more papers, but at least a portion of the additional submissions should lead to a growth in journal content and quality.

  8. Note & Recensioni

    Directory of Open Access Journals (Sweden)

    2014-12-01

    Full Text Available VolumiVito Campanelli, Web Aesthetics. How Digital Media Affect Culture and Society; Id., Remix It Yourself. Analisi socio-estetica delle forme comunicative del Web [Emanuele Crescimanno] • Jean-François Bordron, Image et vérité. Essais sur les dimensions iconiques de la connaissance [Veronica Estay Stange] • Stéphane Dumas, Les peaux créatrices – Esthétique de la sécrétion [Marc-Vincent Howlett]NoteQuidam veritatis effectus. A proposito di A Plea for Balance in Philosophy. Essays in honour of Paolo Parrini [Fabrizio Desideri] • A Single Face to Capture the Whole World: Literary Shapes and Shadows. An Interview With Tolm Coibin [Fabrizia Abbate] •Convegno Le sensible a l’oeuvre: savoirs du corps entre esthetique et neurosciences, Parigi, 15 Maggio 2014 [Jessica Murano] 

  9. The Practice of Designing Qualitative Research on Educational Leadership: Notes for Emerging Scholars and Practitioner-Scholars

    Science.gov (United States)

    Knapp, Michael S.

    2017-01-01

    This article addresses a gap in methodological writing, concerning typical practice in designing qualitative inquiry, especially in research on educational leadership. The article focuses on how qualitative research designs are actually developed and explores implications for scholars' work, especially for new scholars and for methods teachers.…

  10. Editor's Note

    African Journals Online (AJOL)

    Responding to suggestions from the research fellowship of the Institute of African Studies for a re-branding of the Research Review, which began publication in the early 1960s soon after the establishment of the Institute, the old title has now been replaced with a new title — Contemporary Journal of African Studies. This is ...

  11. Editor's Note

    African Journals Online (AJOL)

    It may be recalled that responding to suggestions from the research fellowship of the Institute of African Studies for a re-branding of the Research Review, which began publication in the early 1960s soon after the establishment of the Institute, the old title was replaced in 2012 by this new title — Contemporary Journal of ...

  12. Editor's Note

    African Journals Online (AJOL)

    It is essential to recall that responding to suggestions from the research fellowship of the Institute of African Studies for a re-branding of the Research Review, which began publication in the early 1960s soon after the establishment of the Institute, the old title has been replaced with this new title — Contemporary Journal of ...

  13. Concept Note

    International Development Research Centre (IDRC) Digital Library (Canada)

    Boby

    2010-03-18

    Mar 18, 2010 ... International Development Research Centre (IDRC) ... Cameroon last year by capitalizing on lessons learned to forge partnerships and cooperation initiatives ... prizes awarded three (3) months after the training workshop. IV.

  14. Editor's note

    Directory of Open Access Journals (Sweden)

    Editor’s Note

    2006-11-01

    Full Text Available A “Thousand Words” is a new essay feature of the CODATA Data Science Journal. Each of these essays is intended to paint a picture of a thousand words of a subject important to data scientists. It is opinion – not research, but nevertheless of interest and importance to understanding the fundamental role data science plays in today’s scientific research and development

  15. Physicians’ Progress Notes

    DEFF Research Database (Denmark)

    Bansler, Jørgen; Havn, Erling C.; Mønsted, Troels

    2013-01-01

    in patient care, they have not dealt specifically with the role, structure, and content of the progress notes. As a consequence, CSCW research has not yet taken fully into account the fact that progress notes are coordinative artifacts of a rather special kind, an open-ended chain of prose texts, written...... sequentially by cooperating physicians for their own use as well as for that of their colleagues. We argue that progress notes are the core of the medical record, in that they marshal and summarize the overwhelming amount of data that is available in the modern hospital environment, and that their narrative...... format is uniquely adequate for the pivotal epistemic aspect of cooperative clinical work: the narrative format enables physicians to not only record ‘facts’ but also—by filtering, interpreting, organizing, and qualifying information—to make sense and act concertedly under conditions of uncertainty...

  16. Subjectivity in Education and Health: Research Notes on School Learning Area and Physical Education in Mental Health

    Science.gov (United States)

    Bezerra, Marilia; da Costa, Jonatas Maia

    2016-01-01

    This paper presents the results of two studies researching the theory of subjectivity from a cultural-historical perspective. The studies are situated in the fields of education and health and are conducted using Qualitative Epistemology. The first study discusses the pathological movement problems of learning disabilities in Brazilian schools and…

  17. Young Travellers and the Children's Fund: Some Practical Notes on an Experimental Image-Based Research Project

    Science.gov (United States)

    Dean, Charlotte

    2007-01-01

    The paper reflects on how photography was used during a small piece of mixed methods qualitative research with some young Travellers and their families. The context for the study was "Every Child Matters" and the accompanying push aimed to include groups considered as marginalised, such as young Travellers, in mainstream educational provision, as…

  18. Editor's Note

    Directory of Open Access Journals (Sweden)

    Francisco Mochón

    2018-03-01

    Full Text Available The International Journal of Interactive Multimedia and Artificial Intelligence - IJIMAI (ISSN 1989 - 1660 provides an interdisciplinary forum in which scientists and professionals can share their research results and report new advances on AI tools or tools that use AI with interactive multimedia techniques.

  19. Electronic Notes.

    Science.gov (United States)

    Krauthamer, Helene

    2001-01-01

    Describes a workshop used with classes doing Web research for their English papers in a computer lab. Shows how this is a good opportunity for students to learn to find, evaluate, and save Web sources, how to read critically and annotate the sources, and how to weave them into working drafts and avoid plagiarism. (SR)

  20. Editor's note

    African Journals Online (AJOL)

    remains uncontested”, and confirms: “However, the translation of this recommendation into successful practice continues to fall short”. Uncontested as the benefits ... Education and Training,1 is a revision of the Policy and procedures for measurement of research output of public higher education institutions (2003),2 and will ...

  1. EDITORIAL NOTE

    African Journals Online (AJOL)

    Mugumbate

    of programme communication variables in community development projects. ... undergone some changes owing to devastating effects of HIV and. AIDS and the ... new family set up: the child headed household (CHH). This research ... Therefore the study examined how effective music therapy was in the psychosocial ...

  2. WELCOME NOTE

    Directory of Open Access Journals (Sweden)

    Srinivas Mondam

    2016-12-01

    Full Text Available On behalf of the honorable editors, Executive chief editor and all the editorial board members, I would like to warmly welcome you to the launch of International Journal of Physiotherapy. It is both an inspiring and a challenging task for taking up editorship of the International Journal of Physiotherapy. The mission of International Journal of Physiotherapy is to bring together researchers from all disciplines involved in physiotherapy in an atmosphere, where ideas and experiences can be shared and discussed. Improving the quality and effectiveness of physiotherapy interventions and services is one of the central aims of the International Journal of Physiotherapy. This journal is committed to playing an active role and to support the development of physiotherapy. We are delighted to have you to share your experience and to contribute to the development of quality standards in the field of physiotherapy. This journal offers a first opportunity to publish the findings of your study. International Journal of Physiotherapy also gives an opportunity to learn about different approaches to translate standards into practice. The journal provides a platform for scientific exchange of information which will prove beneficial for academicians, clinical researchers and students. IJPHY will publish the major breakthroughs and developments in the field of physiotherapy – from clinical practice to research and technology. Recent years have brought us researchers who have contributed to the remarkable development and striking discoveries in the field of physiotherapy that have sprung from rapid advances in sequencing technologies, approaches and various analytical techniques that can be used in the various fields of physiotherapy. I think we can all look forward to some interesting insights from the physiotherapists from various parts of the world. IJPHY will receive articles reporting original research, reviews and case reports. We believe that you will

  3. Editor's Note

    Directory of Open Access Journals (Sweden)

    Daniel Shanahan

    2017-01-01

    Full Text Available THIS issue marks the second of two special issues focusing on corpus approaches to musicological and music-theoretical research. What began as a simple call for papers turned into a fairly ambitious project, and I couldn't be more pleased with the result. The range and depth of the articles in these two issues demonstrates the level of scholarly work being conducted in this growing area of research. In this issue, Derek Strykowski's article on text-painting in Marenzio's madrigals provides a model for how one might search address the often difficult-to-test notion of text-painting, and Craig Sapp's commentary is a nice supplemental discussion of the topic, offering avenues of future research. Fernando Benadon's analysis of roughly 30,000 onset times in Afro-Cuban drumming focuses on the notion of "near onsets" and "onset clusters", and Peter Marten's commentary argues that, while this might be difficult to perceive, the methodology demonstrates an excellent model for future research. Steven Cannon's analysis of the form of 19th-century symphonies is enlightening, and provides a nice empirical backing to much of the extant literature on the repertoire, and Ben Duane's response provides a nice commentary on future directions and possible methodological improvements. Caitlyn Trevor and David Huron's article on the usage of open strings in nominally sad music deftly bridges emotion research and instrumental affordances, and Daniel Müllensiefen's commentary praises the methodology, laments the negative results, and issues a call for more negative results to be published. Perhaps the most unique aspect of Empirical Musicology Review is the "open peer review" format it pioneered a decade ago. This issue contains a number of commentaries —and responses to commentaries— that fit within that vision. Nat Condit-Schultz and Mitch Ohriner take two fascinating but different approaches to the notion of "flow" in hip-hop, and provide insightful

  4. Forum Notes: The 1999 environmental research and technology development forum for the upstream oil and gas industry

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-02-04

    This conference provided an opportunity to exchange information on current research and development projects regarding the environment and the petroleum industry. In addition to summaries of the various sessions, the booklet also includes a list of registrants and poster session participants. The conference was divided into the following three sessions: (1) site remediation, site restoration and groundwater, (2) ecological health, cumulative effects and agriculture, and (3) flaring, odour and greenhouse gas emissions. The session on site remediation heard papers that dealt with feedback on existing projects including soil quality guidelines for crude oil in agricultural soils, hydrophobicity, assessment of phytoremediation as an in-situ technique for cleaning sites polluted by oil, remediation using wetlands, land application of bioremediated soils, and flare pit characterization. The session on ecological health was treated to presentations describing research and development projects including the Eastern Slopes Grizzly Bear Project, the Native Prairie Revegetation Project, and the Caroline Livestock Study. The session on flaring and greenhouse gas emissions described research and development projects on dehydrator benzene emissions, monitoring of odour causing compounds, flare speciation, flare performance, improved liquid separation, alternate technologies, improved measurement of methane fugitive emissions from gas operations, and methane emissions from heavy oil operations.

  5. Forum Notes: The 1999 environmental research and technology development forum for the upstream oil and gas industry

    International Nuclear Information System (INIS)

    1999-01-01

    This conference provided an opportunity to exchange information on current research and development projects regarding the environment and the petroleum industry. In addition to summaries of the various sessions, the booklet also includes a list of registrants and poster session participants. The conference was divided into the following three sessions: (1) site remediation, site restoration and groundwater, (2) ecological health, cumulative effects and agriculture, and (3) flaring, odour and greenhouse gas emissions. The session on site remediation heard papers that dealt with feedback on existing projects including soil quality guidelines for crude oil in agricultural soils, hydrophobicity, assessment of phytoremediation as an in-situ technique for cleaning sites polluted by oil, remediation using wetlands, land application of bioremediated soils, and flare pit characterization. The session on ecological health was treated to presentations describing research and development projects including the Eastern Slopes Grizzly Bear Project, the Native Prairie Revegetation Project, and the Caroline Livestock Study. The session on flaring and greenhouse gas emissions described research and development projects on dehydrator benzene emissions, monitoring of odour causing compounds, flare speciation, flare performance, improved liquid separation, alternate technologies, improved measurement of methane fugitive emissions from gas operations, and methane emissions from heavy oil operations

  6. Theoretical and methodological notes on visual and audiovisual sources in researches on Life Stories and Self-referential Memorials

    Directory of Open Access Journals (Sweden)

    Maria Helena Menna Barreto Abrahão

    2014-01-01

    Full Text Available The text explicits the reflection that bases the use of pictures, films and videofilms as sources in research on Life Stories and Self-re- ferential Memorials in Teachers’ Education. After refering to the researches in which we use this support since 1988, we work with two complementary pairs of theoretical dimensions of narratives in visual and audiovisual sources and their use in such empirical rese- arch: subjectivity/truth and space/time. These dimensions are worked grounded in Barthes (1984 to propose an interpretative effort of these sources to understand the essence of photography according to the photographer and the essence of photography according to the photographed person concocted to the essence of photography according to the researcher. The Barthesian constructs studium and punctum are applied to reading the narratives of the filmic and pho- tographic material, reaching the more radical expression of the Bar- thesian puctum: real or representational death of the referent that serves the photos and movies. The discussion of these dimensions for the analysis of (audio visual sources is complemented with the support of several other authors.

  7. Editor's Note

    Directory of Open Access Journals (Sweden)

    Ellen R. Cohn

    2012-06-01

    Full Text Available The current issue of the International Journal of Telerehabilitation (IJT contains original research evaluating the efficacy of telerehabilitation; an evaluation of a telerehabilitation system for community based research; and work that examines users’ perceptions of the privacy and security of the three top used consumer-based, free Voice and video over the Internet Protocol (VoIP software systems (e.g., Skype  and others used by health care providers to deliver therapeutic services via telerehabilitation. Two Brief Communications authored by speech-language pathologists present the possibilities for significant telehealth related cost-savings when telepractice is implemented in clinical practice.  Finally, this issue presents a report generated by a working group within the American Telemedicine Association’s Telerehabilitation Special Interest Group, and announcements from the American Telemedicine Association and the International Society for Augmentative and Alternative Communication (ISAAC.

  8. WELCOME NOTE

    OpenAIRE

    Srinivas Mondam

    2016-01-01

    On behalf of the honorable editors, Executive chief editor and all the editorial board members, I would like to warmly welcome you to the launch of International Journal of Physiotherapy. It is both an inspiring and a challenging task for taking up editorship of the International Journal of Physiotherapy. The mission of International Journal of Physiotherapy is to bring together researchers from all disciplines involved in physiotherapy in an atmosphere, where ideas and experiences can be ...

  9. Confirmation bias in studies of nestmate recognition: a cautionary note for research into the behaviour of animals.

    Directory of Open Access Journals (Sweden)

    Ellen van Wilgenburg

    Full Text Available Confirmation bias is a tendency of people to interpret information in a way that confirms their expectations. A long recognized phenomenon in human psychology, confirmation bias can distort the results of a study and thus reduce its reliability. While confirmation bias can be avoided by conducting studies blind to treatment groups, this practice is not always used. Surprisingly, this is true of research in animal behaviour, and the extent to which confirmation bias influences research outcomes in this field is rarely investigated. Here we conducted a meta-analysis, using studies on nestmate recognition in ants, to compare the outcomes of studies that were conducted blind with those that were not. Nestmate recognition studies typically perform intra- and inter colony aggression assays, with the a priori expectation that there should be little or no aggression among nestmates. Aggressive interactions between ants can include subtle behaviours such as mandible flaring and recoil, which can be hard to quantify, making these types of assays prone to confirmation bias. Our survey revealed that only 29% of our sample of 79 studies were conducted blind. These studies were more likely to report aggression among nestmates if they were conducted blind (73% than if they were not (21%. Moreover, we found that the effect size between nestmate and non-nestmate treatment means is significantly lower in experiments conducted blind than those in which colony identity is known (1.38 versus 2.76. We discuss the implications of the impact of confirmation bias for research that attempts to obtain quantitative synthesises of data from different studies.

  10. Confirmation bias in studies of nestmate recognition: a cautionary note for research into the behaviour of animals.

    Science.gov (United States)

    van Wilgenburg, Ellen; Elgar, Mark A

    2013-01-01

    Confirmation bias is a tendency of people to interpret information in a way that confirms their expectations. A long recognized phenomenon in human psychology, confirmation bias can distort the results of a study and thus reduce its reliability. While confirmation bias can be avoided by conducting studies blind to treatment groups, this practice is not always used. Surprisingly, this is true of research in animal behaviour, and the extent to which confirmation bias influences research outcomes in this field is rarely investigated. Here we conducted a meta-analysis, using studies on nestmate recognition in ants, to compare the outcomes of studies that were conducted blind with those that were not. Nestmate recognition studies typically perform intra- and inter colony aggression assays, with the a priori expectation that there should be little or no aggression among nestmates. Aggressive interactions between ants can include subtle behaviours such as mandible flaring and recoil, which can be hard to quantify, making these types of assays prone to confirmation bias. Our survey revealed that only 29% of our sample of 79 studies were conducted blind. These studies were more likely to report aggression among nestmates if they were conducted blind (73%) than if they were not (21%). Moreover, we found that the effect size between nestmate and non-nestmate treatment means is significantly lower in experiments conducted blind than those in which colony identity is known (1.38 versus 2.76). We discuss the implications of the impact of confirmation bias for research that attempts to obtain quantitative synthesises of data from different studies.

  11. Editor's Note

    Directory of Open Access Journals (Sweden)

    Daniel Shanahan

    2016-07-01

    Full Text Available CORPUS studies have increasingly been of interest to music theorists, musicologists, and music psychology researchers, as is evident in the sheer number of excellent submissions to this special issue. The breadth and depth of these articles, as well as the insightful commentaries, make it seem only fitting to publish over two issues. The current issue contains five articles, eight commentaries, and one research report, and covers folk music, post-tonal music, jazz, Western art music, and the popular music found on Youtube. Eerola presents a model of melodic entropy that provides a nice framework for future information-theoretic work, while Roger Dean and Marcus Pearce present a new approach to modelling pitch-structure in post-tonal music. Frieler, Pfleiderer, Abeßer, and Zaddach give an analysis of jazz solos that demonstrates the role of a narrative arcs in improvisation. White and Quinn present a new corpus that makes use of vast compilations of web-based MIDI data, and demonstrates how such a corpus might be facilitate future musicological and music-theoretic research. Plazak focuses on Youtube as a corpus, and argues that the mutability of such a corpus facilitates our understanding of musical communication, as well as the important role of the listener in defining and re-defining such a dataset. It's encouraging to me both as an editor and practitioner of corpus methods that so many of those heavily involved with the field contributed to these two issues as either an author or a commentator. This issue also brings a number of changes on other fronts. Firstly, Nicola Dibben and Renee Timmers, who served as the journal's editors since Volume 7, have completed their editorial terms. They oversaw the transformation of the journal into a new Open Journal Systems platform, and were able to consistently produce issues that set a very high standard. Under their leadership, the journal both increased its accessibility (we now average more than 1

  12. Editors’ Note

    Directory of Open Access Journals (Sweden)

    Heller WD

    2014-12-01

    Full Text Available The unexpected death of Richard Baker at Easter of this year was a shock for all who knew him. It was just in the last Issue of the Journal (Volume 22, No. 4, January 2007 that we as Editors were pleased and proud to print the laudation by Thomas A. Perfetti of Richard Baker who received the 2006 Tobacco Science Research Conference Lifetime Achievement Award. In this Issue, we have the sad duty to print an obituary for Richard Baker. We have asked Ray Thornton, a former Scientific and Regulatory Manager at BAT to write the obituary, because Ray was a colleague, neighbour and friend of Richard Baker.

  13. Research Notes ~ Combating HIV/AIDS Epidemic in Nigeria: Responses from National Open University of Nigeria (NOUN

    Directory of Open Access Journals (Sweden)

    Terhemba Nom Ambe-Uva

    2007-11-01

    Full Text Available Universities have come under serious attack because of their lackluster response to HIV/AIDS. This article examines the response of National Open University of Nigeria (NOUN and its strategic responses in combating HIV/AIDS epidemic. This is achieved by examining NOUN’s basic structures that position the University to respond to the epidemic; and second, by assessing HIV/AIDS strategies and policy framework the University has put in place. An interpretative epistemological stance was used for this study, and a qualitative research involving focus group discussion (FGD and analysis of secondary data was carried out. Results showed that NOUN has identified the impact the epidemic has on the university, although it has yet to institutionalize an HIV/AIDS policy. NOUN’s Draft Service Charter, however, has identified the fight against HIV/AIDS as a core mandate of the University, and the introduction of HIV/AIDS certification programs can be viewed as proactive policies in response to the epidemic. Results of this study are discussed in terms of their relevance to future research and the impact such policy frameworks may have on combating the epidemic, both within the University and the wider community.

  14. Micropropagation of Ficus religiosa L. via leaf explants and comparative evaluation of acetylcholinesterase inhibitory activity in the micropropagated and conventionally grown plants.

    Science.gov (United States)

    Siwach, Priyanka; Gill, Anita Rani

    2014-10-01

    A high-frequency, season-independent, in vitro regeneration of Ficusreligiosa was developed, followed by comparative acetylcholinesterase inhibitory (AChEI) activity assay of the in vitro raised and conventionally grown plants. The use of AChEI activity is the most accepted strategy for the treatment of Alzheimer disease. Fully expanded, mature leaves were cut into different segments to initiate the cultures. The middle section of the leaf in vertical orientation with cut portion inserted inside the medium was found most suitable for direct shoot regeneration. Leaf explants responded with nearly consistent frequency (60-66.67 %) throughout the year. To obtain high frequency response with enhanced shoot multiplication rate, 32 plant growth regulator regimes were screened amongst which benzylaminopurine at 5.0 mg/l was found most suitable, yielding 100 % response and maximum number of shoots per explant (7.93); same concentration was also most supportive for repeated multiplication (6.53 shoots). The quality of the shoots and multiplication rate could be significantly enhanced (24.35 shoots) when adenine sulphate, glutamine and phloroglucinol, in an optimised concentration, were additionally supplemented. The clonal nature of the micropropagated plants was confirmed by random amplified polymorphic DNA analysis. A comparative analysis of AChEI activity was carried out amongst the methanolic extracts of stem segments of the mother plant, randomly selected seedlings of different age (4 and 6 months old) of the same mother plant and randomly selected micropropagated plants of different age (3 and 6 months age). The mother plant sample showed effective AChEI activity, with IC 50 of 66.46 μg/ml while seedlings, of different age groups, performed poorly (6-month-old seedlings, Se-1 6M , yielded IC 50 of 20,538.46 μg/ml, while two randomly selected 4 months' aged seedlings, Se-2 4M and Se-3 4M exhibited IC 50 of 19,341.03 and 24,281.70 μg/ml). On the other hand

  15. The Effects of Industry Type, Company Size and Performance on Chinese Companies’ IC Disclosure: A Research Note

    Directory of Open Access Journals (Sweden)

    Yi An

    2011-09-01

    Full Text Available This paper examines the effects of industry type, firm size and corporate performance on intellectual capital (IC disclosure among Chinese (mainland companies. It was found that industry type did not have a significant influence on IC reporting practices of Chinese firms; the larger firms generally reported more IC information than the relatively smaller firms; and there was a positive relationship between corporate performance and IC disclosure. This paper contributes to fairly limited literature regarding the associations between the level of IC disclosure and a variety of relevant impact factors, in particular in the Chinese mainland context. In addition, the findings of this research provide some references for policy-makers while developing an IC reporting framework applicable to the Chinese environment.

  16. Editor's note

    Science.gov (United States)

    2016-02-01

    In the Invited Feature Article in this issue of Estuarine, Coastal and Shelf Science, Ruben Kosyan and Violeta Velikova describe the disastrous anthropogenic impact on the Black Sea coast of Bulgaria, Georgia, Romania, Russian Federation, Turkey, and Ukraine over the last 100 years and its inverse correlation with economic development as exemplified by improving water quality during a downturn in the economy. This degradation is now accelerating from the renovation and construction of ports and terminals, the development of resorts, hazardous industrial development, oil and gas exploration, and unsustainable agricultural and fishing practices. There appears to be no enforcement of protected areas. In the absence of scientific research on these issues, coastal zone management appears to be based on 'learning after seeing disastrous effects'. Without recognition of the need for Integrated Coastal Zone Management, the Black Sea coast and the coastal waters will deteriorate, as was observed between the 1970s and the 1990s.

  17. Editors' Note

    Directory of Open Access Journals (Sweden)

    Jana Cason

    2014-09-01

    Full Text Available The spring 2014 issue of the International Journal of Telerehabilitation (IJT contains four informative and timely policy articles: (1 an invited commentary describing the exploratory process underway within physical therapy to create licensure portability for physical therapists, (2 an analysis of state telehealth laws and regulations for occupational therapy and physical therapy, (3 an overview of telehealth evidence and key telehealth policy issues in occupational therapy, and (4 the World Federation of Occupational Therapists’ (WFOT Position Statement on Telehealth. This issue also contains original research evaluating the feasibility of providing pediatric dysphagia treatment via telepractice, a clinical report of student learning outcomes associated with an innovative experiential learning assignment involving (international teleconsultation in a Master of Science in Occupational Therapy (MSOT curriculum, a book review, and announcements from the American Telemedicine Association.

  18. Editors' Note

    Directory of Open Access Journals (Sweden)

    Ellen R. Cohn

    2015-01-01

    Full Text Available The Fall 2014 issue of the International Journal of Telerehabilitation (IJT contains original research that evaluates the role of teletherapy and online language exercises in the treatment of chronic aphasia; investigates whether improvements are maintained after in-home pulmonary telerehabilitation for patients with chronic obstructive pulmonary disease; and studies the use of in-home telerehabilitation for proximal humerus fractures. Within the context of two case studies, an author evaluated three service delivery models (direct, hybrid, and telepractice for stuttering intervention. The results of a preliminary qualitative investigation are presented for telepractice in rural schools in Australia. And, schemas are offered for privacy and security analyses for store and forward applications in physical and occupational therapy. The Fall 2014 issue also contains a book review, and announcements from the American Telemedicine Association and the Mid-Atlantic Telehealth Resource Center.

  19. Notes on policies of research productivity in Brazil: Consequences for academic life, workplace ethics and workers´ health

    Directory of Open Access Journals (Sweden)

    Madel Therezinha Luz

    2009-02-01

    Full Text Available This article is part of a sequence of papers that are fruit of sociological research carried out over the course of a decade on the search for care and health practices and their possible relationship to the labor regimes in contemporary capitalism as well asreflections on the social consequences of educational and scientific and technological policies at the higher education and particularly at the post-graduate level in Brazil. Our reflections have evolved from an initial perception that there is a growing search for ways to care for one’s health in our society to the realization that for a large part of the population, work, which has become merely a job (difficult and precarious has undergone considerable loss of meaning as far as the very act of working is concerned. Furthermore, we perceive the suffering generated by the loss of collective values and meanings around work and being a worker that belonged to a past moment in culture and social life, as well as the loss of the importance and prestige of human labor within the contemporary productive structure, linked to the technological transformations that are currently underway, generating malaise and collective illness. Keywords: public health, productivity, workplace health, work ethics, sociology of health.

  20. Editors' Note

    Directory of Open Access Journals (Sweden)

    Ellen R. Cohn

    2015-11-01

    Full Text Available The Fall 2015 issue of the International Journal of Telerehabilitation (IJT presents the original work of three distinguished and innovative interdisciplinary teams.  Interdisciplinarity is also a hallmark of the IJT editorial staff, reviewers, and publishing team.The first article is a product of engineers with expertise in computing science (LoPresti and Simpson and a medical speech-language pathologist (Jinks who practices in the area of assistive technology. These authors reported upon the degree to which consumers are satisfied with the provision of telerehabilitation services for augmentative and alternative communication or alternative computer accessibility.A second article, by Proffitt, (occupational therapist and Lange, (a physiotherapist with expertise in the use of interactive video games and virtual reality technologies demonstrated the feasibility of employing a 6-week, game-based, in-home telerehabilitation exercise program using the Microsoft Kinect® for individuals with chronic stroke.Finally, the third article co-authored by Watzlaf (health information management, with degrees in public health and epidemiology, DeAlmeida (health information systems, with a degree in cell and molecular biology, Zhou (with degrees in computer science and physics, and expertise in mathematical modeling on health related topics, information integration, and comparative genomics, and Hartman (a reference librarian with a degree in chemistry, who collaborates with faculty in the health sciences and serves as a liaison to a school of health and rehabilitation sciences describes a protocol to conduct systematic reviews of research in telerehabilitation, with the aim that IJT readers can ultimately apply this protocol to identify best practices in telerehabilitation.

  1. An improved micropropagation of Arnebia hispidissima (Lehm.) DC. and assessment of genetic fidelity of micropropagated plants using DNA-based molecular markers.

    Science.gov (United States)

    Phulwaria, Mahendra; Rai, Manoj K; Shekhawat, N S

    2013-07-01

    An efficient and improved in vitro propagation method has been developed for Arnebia hispidissima, a medicinally and pharmaceutically important plant species of arid and semiarid regions. Nodal segments (3-4 cm) with two to three nodes obtained from field grown plants were used as explants for shoot proliferation. Murashige and Skoog's (MS) medium supplemented with cytokinins with or without indole-3-acetic acid (IAA) or naphthalene acetic acid was used for shoot multiplication. Out of different PGRs combinations, MS medium containing 0.5 mg l(-1) 6-benzylaminopurine and 0.1 mg l(-1) IAA was optimal for shoot multiplication. On this medium, explants produced the highest number of shoots (47.50 ± 0.38). About 90 % of shoots rooted ex vitro on sterile soilrite under the greenhouse condition when the base (2-4 mm) of shoots was treated with 300 mg l(-1) of indole-3-butyric acid for 5 min. The plantlets were hardened successfully in the greenhouse with 85-90 % survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro-regenerated plants of A. hispidissima. Out of 40 (25 RAPD and 15 ISSR) primers screened, 15 RAPD and 7 ISSR primers produced a total number of 111 (77 RAPD and 34 ISSR) reproducible amplicons. The amplified products were monomorphic across all the micropropagated plants and were similar to the mother plant. To the best of our knowledge, it is the first report on the assessment of the genetic fidelity in micropropagated plants of A. hispidissima.

  2. Note on current position regarding the development by the UKAEA of Reduced Enrichment fuels for Research and Test Reactors

    International Nuclear Information System (INIS)

    Hickey, B.

    1983-01-01

    The United Kingdom Atomic Energy Authority have an MTR fuel fabrication plant located at Dounreay on the north coast of Scotland. The prime function of the plant is to manufacture fuel elements for the UKAEA's own DIDO and PLUTO heavy water reactors located at their research establishment at Harwell. The plant, which has a capacity of about 1000 fuel elements per annum, also manufactures fuel elements, on a commercial basis, for university reactors in the United Kingdom and for a number of customers in overseas countries. The UKAEA have been manufacturing MTR fuel elements of a wide range of designs for over twenty-five years. Following the initiative of the US Government's RERTR programme, the UKAEA have embarked on a modest programme of MTR fuel manufacturing development., irradiation and post-irradiation examination to establish the techniques required to manufacture fuel elements containing uranium of a significantly lower enrichment than that in the fuel elements they currently manufacture. In the first instance this work is being directed towards the production of fuel elements containing uranium of 45% enrichment. After an initial analysis it was recognised that although a satisfactory 45% enriched version of certain of the designs of fuel elements currently manufactured could probably be produced using established U/Al alloy technology, it would be necessary to utilise powder technology for other elements in order to achieve the higher uranium density required. Studies of published information and consideration of the technology and facilities already available at Dounreay prompted the decision to concentrate on the development Of U 3 O 8 /Al cermet type fuel elements of similar geometry to those currently manufactured. Some of the fuel element designs currently manufactured by the UKAEA are listed: Concentric (Extruded) 74% enriched; Concentric Plates 80% enriched with densities 0.60 and 0.53 g U/ cm 3 ; Flat Plate (Swaged) 80% enriched and Flat Plate

  3. A minimal cost micropropagation protocol for Dianthus caryophyllus L.-- a commercially significant venture.

    Science.gov (United States)

    Pant, Manu

    2016-03-01

    In tissue culture, high production cost of the products restricts their reach. Though tissue culture is a major strength in floriculture it is marred by pricing issues. Hence, we developed a complete regeneration low cost micropropagation protocol for an economically important floriculture crop, carnation (Dianthus caryophyllus L.). Successful regeneration of carnation from nodal explants on cost-efficient medium indicates that psyllium husk, sugar and RO water can effectively replace the conventional medium comprising agar, sucrose and distilled water. The protocol can contribute to increased carnation production at comparatively reduced cost, and there by encourage wide scale adoption by the common growers.

  4. Micropropagation of Plantago asiatica L. through culture of shoot-tips

    Directory of Open Access Journals (Sweden)

    Joanna Makowczyńska

    2011-01-01

    Full Text Available Shoot-tip multiplication of the medicinal species - Plantago asiatica was carried on MS medium with IAA and BAP or kinetin. Best results in micropropagation were achieved by adding 0.1 mg/dm3 IAA and 1 mg/dm3 BAP. After 6 weeks shoots were transferred to MS medium for rooting. The resulting plantlets were transferred after 8 weeks into pots and after a period of adaptation into the ground (field culture. The species Plantago asiatica was propagated in vitro by shoot-tip multiplication for the first time.

  5. Effect of Silver Nitrate DuringEx vitro Acclimatization of Micropropagated Ginger Cultivars

    Directory of Open Access Journals (Sweden)

    Dikash Singh THINGBAIJAM

    2014-03-01

    Full Text Available Silver nitrate (AgNO3 was used under in vitro conditions to study the response of ginger cultivars ‘Nadia’ and ‘Baishey’ under ex vitro. Micropropagated plants treated with AgNO3 showed significant difference (p<0.05 compared to those plantlets without AgNO3 and control type in almost all the different quantitative traits analyzed. Significant difference in number of finger per plant and minirhizome yield indicated the repercussion of AgNO3 during acclimatization.

  6. Editors note

    Directory of Open Access Journals (Sweden)

    Yngve Nordkvelle

    2017-01-01

    title “Networked e-Learning: The changing facilitator - learner relationship, a facilitators’ perspective; A Phenomenological Investigation”. The phenomenological case study deals with how the relationship between facilitator and student is changing. Networked e-Learning is the context and the research is undertaken at an Irish higher education institution.The author’s role as a highly experienced facilitator provides particular and specific insight into the guiding facilitator’s experiences during a time of institutional transition to Networked e-Learning.Gamification is a topic that has been declared as “up and coming” for a number of years. Marc Fabian Buck, of the Nord University, Norway, presents the article “Gamification of learning and teaching in schools – a critical stance”. He states that the aim of Gamification is to change learning for the better by making use of the motivating effects of (digital games and elements typical of games, like experience points, levelling, quests, rankings etc. His most contemporary example is of the “Summer of ‘16” and the apparent success of “Pokemon go”. He argues that gamified learning and teaching suspends the fundamental, subversive, and critical moments only schools can offer.The last article is provided by Ulf Olsson, of Stockholm University, Sweden: “Teachers’ Awareness of Guidelines for Quality Assurance when developing MOOCs”. His study focuses on higher education teachers’ awareness of quality issues in relation to Standards and Guidelines for Quality Assurance in the European Higher Education Area (ESG. Olsson conducted semi-structured interviews with 20 teachers at six Swedish HEIs while they developed open courses (MOOCs. The overall findings show that the teachers were not part of any transparent quality assurance system. Subsequently, he raises the question of the adequacy of a quality system for innovative activities.

  7. Research notes : listening to bridges.

    Science.gov (United States)

    2008-09-01

    The Federal Highway Administration requires owners of structurally deficient bridges to repair, replace, restrict truck loads, or conduct analysis and testing to maintain a safe highway system. Past experiments on reinforced concrete beams showed aco...

  8. OpenLabNotes

    DEFF Research Database (Denmark)

    List, Markus; Franz, Michael; Tan, Qihua

    2015-01-01

    be advantageous if an ELN was Integrated with a laboratory information management system to allow for a comprehensive documentation of experimental work including the location of samples that were used in a particular experiment. Here, we present OpenLabNotes, which adds state-of-the-art ELN capabilities to Open......LabFramework, a powerful and flexible laboratory information management system. In contrast to comparable solutions, it allows to protect the intellectual property of its users by offering data protection with digital signatures. OpenLabNotes effectively Closes the gap between research documentation and sample management......, thus making Open-Lab Framework more attractive for laboratories that seek to increase productivity through electronic data management....

  9. Evaluation of a New Temporary Immersion Bioreactor System for Micropropagation of Cultivars of Eucalyptus, Birch and Fir

    Directory of Open Access Journals (Sweden)

    Edward Businge

    2017-06-01

    Full Text Available The use of liquid instead of solid culture medium for the micropropagation of plants offers advantages such as better access to medium components and scalability through possible automation of the processes. The objective of this work was to compare a new temporary immersion bioreactor (TIB to solid medium culture for the micropropagation of a selection of tree species micropropagated for commercial use: Nordmann fir (Abies nordmanniana (Steven Spach, Eucalyptus (E. grandis x E. urophylla, Downy birch (Betula pubescens Ehrh, and Curly birch (Betula pendula var. carelica. Cultivation of explants in the TIB resulted in a significant increase of multiplication rate and fresh weight of Eucalyptus and B. pendula, but not Betula pubescens. In addition, the fresh weight of embryogenic tissue and the maturation frequency of somatic embryos increased significantly when an embryogenic cell line of A. nordmanniana was cultivated in the TIB compared to solid culture medium. These results demonstrate the potential for scaling up and automating micropropagation by shoot multiplication and somatic embryogenesis in commercial tree species using a temporary immersion bioreactor.

  10. Endogenous cytokinin dynamics in micropropagated tulips during bulb formation process influenced by TDZ and iP pretreatment

    Czech Academy of Sciences Publication Activity Database

    Podwyszynska, M.; Novák, Ondřej; Doležal, Karel; Strnad, Miroslav

    2014-01-01

    Roč. 119, č. 2 (2014), s. 331-346 ISSN 0167-6857 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : Tulipa gesneriana * Endogenous cytokinins * Micropropagation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.125, year: 2014

  11. In vitro propagation of silybum marianum (l.) gaertn. and genetic fidelity assessment of micropropagated plants

    International Nuclear Information System (INIS)

    Lv, Y.W.; Wamg, R.J.; Yang, Z.S.; Wang, Y.J.

    2017-01-01

    Silybum marianum (milk thistle), an annual and biennial herbaceous plant, has been used to treat liver disease for 2000 years. An efficient system for micropropagation from leaf explants of wild-grown S. marianum was successfully established and the genetic fidelity of micropropagated plants was assessed using PCR-based random amplified polymorphic DNA (RAPD). The highest frequency (98.9%) of callus induction was obtained from explants cultivated for 4 weeks on Murashige and Skoog (MS) medium supplemented with 1.5 mg l/sup -1/N6-Benzylaminopurine (BA) and 2.0 mg l/sup -1/ alpha-Naphthaleneacetic acid (NAA). The optimal medium for shoots organogenesis was MS supplemented with 3.0 mg l/sup -1/ Gibberellic acid (GA/sub 3/) and 1.0 mg l/sup -1/NAA, while shoot proliferation was accomplished in MS supplemented with 1.0 mg l/sup -1/BA and 0.3 mg l/sup -1/NAA. Rooting (94.5%) of these shoots was achieved after 4 weeks on 1/2 MS medium supplemented with 1.0 mg l/sup -1/ IBA. A total of 92% of the plants were surviving 6 weeks after transplantation of plantlets to soil. RAPD analysis revealed that the genetic fidelity between the parent and the In vitro-raised plantlets exhibited 100% similarity. This confirmed the true-to-type nature of the In vitro-raised clones. |(author)

  12. Organic amendments enhance Pb tolerance and accumulation during micropropagation of Daphne jasminea.

    Science.gov (United States)

    Wiszniewska, Alina; Muszyńska, Ewa; Hanus-Fajerska, Ewa; Smoleń, Sylwester; Dziurka, Michał; Dziurka, Kinga

    2017-01-01

    The study investigated the effects of organic amendments: pineapple pulp (PP) and agar hydrolyzate (AH), on micropropagation and Pb bioaccumulation and tolerance in a woody shrub Daphne jasminea cultured in vitro. The amendments were analyzed for their content of carbohydrates, phenolic acids, and phytohormones and added at a dose of 10 mL L -1 to the medium containing 1.0 mM lead nitrate. Micropropagation coefficient increased by 10.2-16.6 % in PP and AH variants, respectively. Growth tolerance index increased by 22.9-31.8 % for the shoots and by 60.1-82.4 % for the roots. In the absence of Pb, the additives inhibited multiplication and growth of microplantlets. PP and AH facilitated Pb accumulation in plant organs, especially in the roots. PP enhanced bioconcentration factor and AH improved Pb translocation to the shoots. Adaptation to Pb was associated with increased accumulation of phenolics and higher radical scavenging activity. Medium supplementation, particularly with AH, enhanced antiradical activity of Pb-adapted lines but reduced the content of phenolic compounds. The study results indicated that supplementation with organic amendments may be beneficial in in vitro selection against lead toxicity.

  13. Response of Opuntia stricta Haw micropropagated plants in acclimatization and field

    Directory of Open Access Journals (Sweden)

    Marina Medeiros de Araújo Silva

    2017-07-01

    Full Text Available Forage palm (Opuntia stricta Haw. is a great important crop for the semi-arid region of Brasil. It has been propagated through in vitro culture, by areole activation. This work aimed to determine the response of Opuntia stricta Haw. variety ‘Oreja de Elefante Mexicana’ micropropagated plants during its acclimatization and field planting. Plants were acclimatized in tubes and plastic bags with a mixture of soil and manure, and it were kept in greenhouse for 130 days. Later, the plants kept in tubes were divided into nine treatments, according to their size (between 6 to 14 cm, and transfer to the field. The use of tubes provided a higher survival, height, fresh and dry mass of the cladodes. There was no significant differences between the recipients used for root development. At the end of acclimatization it was observed that plants did not have a uniform development, presenting size variation. After the first 15 days in the field, the majority of the plants with primary shoots were larger than 10 cm. At 60 days the survival was 100%, and all plants presented shoots, even the smallest. However, is recommended that plants larger than 10 cm will be used, due to the better establishment to field conditions. The results demonstrate the feasibility in the use of micropropagated plants for the establishment of forage palm fields. Keywords: cladodes, forage palm, in vitro culture, semi-arid region

  14. Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.

    Directory of Open Access Journals (Sweden)

    Mary E Lucero

    2011-03-01

    Full Text Available Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP analysis amplified sequences from callus homologous to diverse fungal and bacterial taxa. Culturing isolated some seed borne endophyte taxa which could be readily propagated apart from the host. Microbial cells were observed within biofilm-like residues associated with plant cell surfaces and intercellular spaces. Various universal primers amplified both plant and microbial sequences, with different primers revealing different patterns of fungal diversity. Bacterial and fungal TEFAP followed by alignment with sequences from curated databases revealed 7 bacterial and 17 ascomycete taxa in A. canescens, and 5 bacterial taxa in A. torreyi. Additional diversity was observed among isolates and clone libraries. Micropropagated Atriplex retains a complex, intimately associated microbiome which includes diverse strains well poised to interact in manners that influence host physiology. Microbiome analysis was facilitated by high throughput sequencing methods, but primer biases continue to limit recovery of diverse sequences from even moderately complex communities.

  15. Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia.

    Science.gov (United States)

    Muniz, Aleksander Westphal; de Sá, Enilson Luiz; Dalagnol, Gilberto Luíz; Filho, João Américo

    2013-01-01

    In vitro rooting and the acclimatization of micropropagated rootstocks of apple trees is essential for plant development in the field. The aim of this work was to assess the use of rhizobia of Adesmia latifolia to promote rooting and acclimatization in micropropagated Marubakaido apple rootstock. An experiment involving in vitro rooting and acclimatization was performed with four strains of rhizobium and two controls, one with and the other without the addition of synthetic indoleacetic acid. The inoculated treatments involved the use of sterile inoculum and inoculum containing live rhizobia. The most significant effects on the rooting rate, primary-root length, number of roots, root length, fresh-shoot biomass, and fresh-root biomass were obtained by inoculation with strain EEL16010B and with synthetic indole acetic acid. However, there was no difference in the growth of apple explants in the acclimatization experiments. Strain EEL16010B can be used to induce in vitro rooting of the Marubakaido rootstock and can replace the use of synthetic indoleacetic acid in the rooting of this cultivar.

  16. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    Directory of Open Access Journals (Sweden)

    Aurilena de Aviz Silva

    Full Text Available ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 11711, and Herbaspirillum seropedicae HRC 54, five levels of urea (0, 2, 5, 7, and 10g L-1, and five evaluation periods (30, 60, 90, 120, and 150 days following the planting of plantlets in trays, and with four replications of each treatment. An increase in the dried weight of shoots and roots of micropropagated pineapple cv. 'Vitória' plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer.

  17. The role of cytokinins during micropropagation of wych elm

    Czech Academy of Sciences Publication Activity Database

    Malá, J.; Máchová, P.; Cvrčková, H.; Karady, Michal; Novák, Ondřej; Mikulík, Jaromír; Dostál, J.; Strnad, Miroslav; Doležal, Karel

    2013-01-01

    Roč. 57, č. 1 (2013), s. 174-178 ISSN 0006-3134 R&D Projects: GA MZe QI92A247 Grant - others:GA MŠk(CZ) ED0007/01/01 Program:ED Institutional research plan: CEZ:AV0Z50380511 Keywords : benzylaminopurine * chlorophyll fluorescence * meta-topolin Subject RIV: GK - Forestry Impact factor: 1.740, year: 2013

  18. Micropropagation of Salvia wagneriana Polak and hairy root cultures with rosmarinic acid production.

    Science.gov (United States)

    Ruffoni, Barbara; Bertoli, Alessandra; Pistelli, Laura; Pistelli, Luisa

    2016-01-04

    Salvia wagneriana Polak is a tropical species native to Central America, well adapted to grow in the Mediterranean basin for garden decoration. Micropropagation has been assessed from axillary shoots of adult plants using a Murashige and Skoog basal medium, with the addition of 1.33-μM 6-benzylaminopurine for shoot proliferation; the subsequent rooting phase occurred in plant growth regulator-free medium. The plants were successfully acclimatised with high survival frequency. Hairy roots were induced after co-cultivation of leaf lamina and petiole fragments with Agrobacterium rhizogenes and confirmed by PCR. The establishment and proliferation of the selected HRD3 line were obtained in hormone-free liquid medium and the production of rosmarinic acid (RA) was evaluated after elicitation. The analysis of RA was performed by LC-ESI-DAD-MS in the hydroalcoholic extracts. The addition of casein hydrolysate increased the RA production, whereas no enrichment was observed after the elicitation with jasmonic acid.

  19. Efficient micropropagation and assessment of genetic fidelity of Boerhaavia diffusa L- High trade medicinal plant.

    Science.gov (United States)

    Patil, Kapil S; Bhalsing, Sanjivani R

    2015-07-01

    Boerhaavia diffusa L is a medicinal herb with immense pharmaceutical significance. The plant is used by many herbalist, Ayurvedic and pharmaceutical industries for production biopharmaceuticals. It is among the 46 medicinal plant species in high trade sourced mainly from wastelands and generally found in temperate regions of the world. However, the commercial bulk of this plant shows genetic variations which are the main constraint to use this plant as medicinal ingredient and to obtain high value products of pharmaceutical interest from this plant. In this study, we have regenerated the plant of Boerhaavia diffusa L through nodal explants and evaluated genetic fidelity of the micropropagated plants of Boerhaavia diffusa L with the help of random amplified polymorphic DNA (RAPD) markers. The results obtained using RAPD showed monomorphic banding pattern revealing genetic stability among the mother plant and in vitro regenerated plants of Boerhaavia diffusa L.

  20. In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India.

    Science.gov (United States)

    Bisht, Shivani; Bisht, N S; Bhandari, Snehlata

    2012-01-01

    An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25-10.0 mgl(-1)) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5-1.0 mgl(-1)) auxins (NAA/IBA/IAA). 1.0 mgl(-1) BAP with 0.5 mgl(-1) NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl(-1) NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.

  1. Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.

    Science.gov (United States)

    Vilanova Neta, Jaci Lima; da Silva Lédo, Ana; Lima, Aloisio André Bonfim; Santana, José Carlos Curvelo; Leite, Nadjma Souza; Ruzene, Denise Santos; Silva, Daniel Pereira; de Souza, Roberto Rodrigues

    2012-09-01

    The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.

  2. Zearalenone Uptake and Biotransformation in Micropropagated Triticum durum Desf. Plants: A Xenobolomic Approach.

    Science.gov (United States)

    Rolli, Enrico; Righetti, Laura; Galaverna, Gianni; Suman, Michele; Dall'Asta, Chiara; Bruni, Renato

    2018-02-14

    A model was set up to elucidate the uptake, translocation, and metabolic fate of zearalenone (ZEN) in durum wheat. After treatment with ZEN, roots and shoots were profiled with LC-HRMS. A comprehensive description of in planta ZEN biotransformation and a biotechnological evaluation of the model were obtained. Up to 200 μg ZEN were removed by each plantlet after 14 days. Most ZEN and its masked forms were retained in roots, while minimal amounts were detected in leaves. Sixty-two chromatographic peaks were obtained, resulting in 7 putative phase I and 18 putative phase II metabolites. ZEN16Glc and ZEN14Glc were most abundant in roots, sulfo-conjugates and zearalenol derivatives were unable to gain systemic distribution, while distinct isomers of malonyl conjugates were found in leaves and roots. This study underlines the potential ZEN occurrence in plants without an ongoing Fusarium infection. Micropropagation may represent a tool to investigate the interplay between mycotoxins and wheat.

  3. Métodos de micropropagação de abacaxizeiro Micropropagation methods of pineapple

    Directory of Open Access Journals (Sweden)

    Adriano Bortolotti da Silva

    2007-09-01

    Full Text Available O objetivo deste trabalho foi comparar diferentes métodos de micropropagação in vitro de abacaxizeiro em larga escala. Os tratamentos utilizados foram micropropagação convencional em meio sólido (5 e 6 g L-1 de ágar e em meio líquido, e micropropagação em biorreator de imersão temporária - imersão no meio de cultura a cada 2, 4 e 8 horas, por 3 min - e contínua, com aeração a cada 2, 4 e 8 horas, por 3 min. Utilizou-se meio MS suplementado com 1 mg L-1 de BAP, 0,25 mg L-1 de ANA, pH ajustado para 5,8. As culturas foram mantidas em sala de crescimento com 25±1ºC, sob luz branca fria (40 µmol m-2 s-1 , com 16 horas de fotoperíodo. O delineamento foi inteiramente casualizado, com nove tratamentos e quatro repetições. Depois de 45 dias de cultivo, foram avaliados número de brotos, brotações superiores a 1 cm, comprimento de brotos, massa de matéria fresca e massa de matéria seca de brotos. Sistemas de imersão temporária, com as plântulas imersas a cada 2 horas por 3 min, proporcionaram maior número, altura e massa de matéria seca de brotos de abacaxizeiro. O sistema de imersão temporária é o método mais eficiente na micropropagação de abacaxizeiro em larga escala.The objective of this work was to compare different methods of pineapple in vitro mass micropropagation. The treatments were conventional micropropagation in solid medium (5 and 6 g L-1 agar and in liquid medium, micropropagation in bioreactor of temporary immersion (immersion in culture medium each 2, 4 and 8 hours, for 3 minutes and continuous immersion (with aeration each 2, 4 and 8 hours, for 3 minutes. MS medium supplement with 1 mg L-1 BAP, 0.25 mg L-1 NAA, and pH adjusted to 5.8 was utilized. Cultures were incubated at 25±1ºC under cool white fluorescent tubes (40 µmol m-2 s-1 with a 16-hours photoperiod. The experiment design was completely randomized, comprising nine treatments and four replicates. After 45 days, number of shootings

  4. Micropropagation of Dianthus deltoides L. through shoot tip and nodal cuttings culture

    Directory of Open Access Journals (Sweden)

    Marković Marija

    2013-01-01

    Full Text Available Micropropagation (shoot tip and nodal cuttings culture was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L. in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP and naphthaleneacetic acid (NAA. The highest multiplication rate was achieved on a medium containing 0.1 mgL-1 of BAP and 0.1 mgL-1 of NAA. The rooting was successful on a hormone-free medium (100%, and the highest percentage of microplant acclimatization (97% was recorded in a 4: 1 mixture of peat and sand. [Projekat Ministarstva nauke Republike Srbije, br. TR 31041: Establishment of wood plantations intended for a forestation of Serbia

  5. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’

    Directory of Open Access Journals (Sweden)

    Kamnoon Kanchanapoom

    2010-01-01

    Full Text Available A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’ was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

  6. Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites

    Directory of Open Access Journals (Sweden)

    Barbara Thiem

    2014-01-01

    Full Text Available A method was devised for the micropropagation of eight species of the genus Oenothera L. from shoot tips and shoot segments with nodes. Microshoot cultures were obtained from explants on Murashige and Skoog (MS medium enriched with IAA and BA. Numerous shoots developed properly after they had been transferred onto MS medium without BA. When they had rooted under the influence of auxins (IAA or IBA, shoots were transferred to pots and then to the soil, where they matured. In the seeds produced by these plants, the content of fatty acids was determined using the GC method. A preliminary analysis of flavonoid compounds and phenolic acids was made using the 2D TLC method (fingerprinting in microshoots and leaves of soil plants regenerated in vitro.

  7. Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.).

    Science.gov (United States)

    Quiroz, Karla A; Berríos, Miguel; Carrasco, Basilio; Retamales, Jorge B; Caligari, Peter D S; García-Gonzáles, Rolando

    2017-06-02

    Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3-6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.

  8. Micropropagation of Cyclopia genistoides, an endemic South African plant of economic importance.

    Science.gov (United States)

    Kokotkiewicz, Adam; Luczkiewicz, Maria; Hering, Anna; Ochocka, Renata; Gorynski, Krzysztof; Bucinski, Adam; Sowinski, Pawel

    2012-01-01

    An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 microM 6-(gamma,gamma-dimethylallylamino)purine (2iP) and 1.0 microM thidiazuron (TDZ). Maximum shoot multiplication rate [(8.2 +/- 1.3) microshoots/explant)] was observed on this medium composition. Prior to rooting, the multiplied shoots were elongated for 60 days (two 30-days passages) on SH medium with one-half sucrose concentration, supplemented with 4.92 microM indole-3-butyric acid (IBA). The rooting of explants was only possible in the case of the elongated shoots. The highest root induction rate (54.8%) was achieved on solid SH medium with one-half sucrose and one-half potassium nitrate and ammonium nitrate concentration, respectively, supplemented with 28.54 microM indole-3-acetic acid (IAA) and 260.25 microM citric acid. The plantlets were acclimatized for 30 days in the glasshouse, with the use of peat/gravel/perlite substrate (1:1:1). The highest acclimatization rate (80%) was obtained for explants rooted with the use of IAA-supplemented medium. The phytochemical profile of the regenerated plants was similar to that of the reference intact plant material. HPLC analyses showed that C. genistoides plantlets obtained by the micropropagation procedure kept the ability to produce xanthones (mangiferin and isomangiferin) and the flavanone hesperidin, characteristic of wild-growing shrubs.

  9. [Study on Rapid Micropropagation in Vitro Technique of Guangfeng Medicinal Yam (Dioscorea opposita) Plantlets].

    Science.gov (United States)

    Yin, Ming-Hua; Xu, Zhi-Jian; Zhang, Sheng-qin; Lv, Si-Jie; Zeng, Yan-hong; Fu, You-zhang; Hong, Sen-rong

    2015-11-01

    In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied. Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected. The best disinfection procedure of stems with a bud of Guangfeng medicinal yam was washed with sterile water for three times after sterilized with 70% alcohol for 20 - 30 s and then washed with sterile water for three times again after sterilized with 0.1% mercuric chloride for 10 - 12 min; The best explants of stems with a bud of Guangfeng medicinal yam was slightly woody and more mature stems witha bud; The best proliferation culture medium of stems with a bud of Guangfeng medicinal yam was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L; The best rooting culture medium of stems with a bud of Guangfeng medicinal yam was MS + NAA 0.5 mg/L; The best culture method of Guangfeng medicinal yam plantlets was liquid culture; The best transplanting matrix of Guangfeng medicinal yam plantlets was the mixture of paddy clay and fine sand (1: 2) or the mixture of perlite and vermiculite (1: 2); The DNA content between Guangfeng medicinal yam plantlets and its potted seedlings had no significant difference. A fast and efficient micropropagation in vitro technological system of stems with a bud of Guangfeng medicinal yam is established, and the flow cytometry detect results also show the genetic stability of Guangfeng medicinal yam plantlets, whose results provide the technical and theoretical basis for the large-scale production of Guangfeng medicinal yam plantlets.

  10. Note Taking and Recall

    Science.gov (United States)

    Fisher, Judith L.; Harris, Mary B.

    1974-01-01

    To study the effect of note taking and opportunity for review on subsequent recall, 88 college students were randomly assigned to five treatment groups utilizing different note taking and review combinations. No treatment effects were found, although quality of notes was positively correlated with free recall an multiple-choice measures.…

  11. In vitro micropropagation and mycorrhizal treatment influences the polyphenols content profile of globe artichoke under field conditions.

    Science.gov (United States)

    Pandino, Gaetano; Lombardo, Sara; Antonino, Lo Monaco; Ruta, Claudia; Mauromicale, Giovanni

    2017-09-01

    The commercial importance of plant tissue culture has grown in recent years, reflecting its application to vegetative propagation, disease elimination, plant improvement and the production of polyphenols. The level of polyphenols present in plant tissue is influenced by crop genotype, the growing environment, the crop management regime and the post-harvest processing practice. Globe artichoke is a significant component of the Mediterranean Basin agricultural economy, and is rich in polyphenols (phenolic acids and flavones). Most commercially grown plants are derived via vegetative propagation, with its attendant risk of pathogen build-up. Here, a comparison was drawn between the polyphenol profiles of conventionally propagated and micropropagated/mycorrhized globe artichoke plants. Micropropagation/mycorrhization appeared to deliver a higher content of caffeoylquinic acids. The accumulation of these compounds, along with luteolin and its derivatives, was not season-dependent. Luteolin aglycone was accumulated preferentially in the conventionally propagated plants. Overall, it appeared that micropropagation/mycorrhization enhanced the accumulation of polyphenols. Copyright © 2017. Published by Elsevier Ltd.

  12. Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study.

    Science.gov (United States)

    Badalamenti, Ornella; Carra, Angela; Oddo, Elisabetta; Carimi, Francesco; Sajeva, Maurizio

    2016-01-01

    Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Ehrenberg, an Appendix I CITES listed species critically endangered and sought after by the horticultural trade, on micropropagated Opuntia ficus-indica Miller. Apical and sub-apical scions of P. aselliformis were used to perform micrografting with a successful rate of 97 and 81 % respectively. Survival rate after ex vivo transfer was 85 %. We hypothesize that this method could be applied to other endangered, slow growing taxa of Cactaceae thus contributing to the conservation of this endangered family.

  13. Rooting of hybrid clones of Populus tremula L. x P. tremuloides Michx. by stem cuttings derived from micropropagated plants

    Energy Technology Data Exchange (ETDEWEB)

    Qibin Yu [Univ. of Helsinki (Finland). Dept. of Plant Biology; Maentylae, N. [Univ. of Turku (Finland). Dept. of Biology, Plant Physiology and Molecular Biology; Salonen, M. [Finnish Forest Research Inst., Laeyliaeinen (Finland). Haapastensyrjae Breeding Station

    2001-07-01

    Propagation costs could be cut by replacing part of the micropropagation process with steps involving more traditional techniques. This study explored possibilities for improving existing vegetative propagation techniques for aspen using stem cuttings obtained from micropropagated plants. Vegetative propagation through stem cuttings was studied in 10 micropropagated hybrid aspen clones (Populus tremula L. x P. tremuloides Michx). Cuttings containing one axillary bud were harvested from the same donor plants twice during the growing season: the first harvest in May and the second harvest in July. Rooting percentage was correlated positively with root length, number of roots and height of cutting plant but negatively with length of rooting. The average rooting percentage was 53% in the first harvest and 27% in second harvest. Indole-3-butyric acid treatments (1.2 mM) significantly improved rooting in the second harvest, but not in the first harvest, suggesting different endogenous auxin levels in the cuttings. A significant variation for most traits related to rooting ability was found among the clones, indicating that clonal effects play an important role in the propagation of aspen. Thus, clones with a good response in shoot growth and rooting could be exploited in large-scale propagation using stem cuttings.

  14. Leading a Classroom Discussion: Definition, Supportive Evidence, and Measurement of the "ETS"® National Observational Teaching Examination (NOTE) Assessment Series. Research Memorandum No. RM-16-09

    Science.gov (United States)

    Witherspoon, Margaret; Sykes, Gary; Bell, Courtney

    2016-01-01

    This paper provides a description and rationale for a performance assessment of a teaching practice--leading a classroom discussion (LCD)--included in the ETS® National Observational Teaching Examination (NOTE) assessment series. In this assessment, candidates interact with a small class of virtual students represented by avatars in a…

  15. 1. On note taking.

    Science.gov (United States)

    Plaut, Alfred B J

    2005-02-01

    In this paper the author explores the theoretical and technical issues relating to taking notes of analytic sessions, using an introspective approach. The paper discusses the lack of a consistent approach to note taking amongst analysts and sets out to demonstrate that systematic note taking can be helpful to the analyst. The author describes his discovery that an initial phase where as much data was recorded as possible did not prove to be reliably helpful in clinical work and initially actively interfered with recall in subsequent sessions. The impact of the nature of the analytic session itself and the focus of the analyst's interest on recall is discussed. The author then describes how he modified his note taking technique to classify information from sessions into four categories which enabled the analyst to select which information to record in notes. The characteristics of memory and its constructive nature are discussed in relation to the problems that arise in making accurate notes of analytic sessions.

  16. Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

    Directory of Open Access Journals (Sweden)

    Marković Marija

    2015-01-01

    Full Text Available The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%, but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%. However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution. [Projekat Ministarstva nauke Republike Srbije, br. 43007

  17. Micropropagation and subsequent enrichment of carotenoids, fatty acids and tocopherol contents in Sedum dasyphyllum L

    Science.gov (United States)

    Park, Han Yong; Saini, Ramesh Kumar; Gopal, Judy; Keum, Young-Soo; Kim, Doo Hwan; Lee, Onew; Sivanesan, Iyyakkannu

    2017-10-01

    A promising micropropagation protocol has been systematically established and demonstrated for the enhanced production of carotenoids, tocopherol and fatty acids in shoot tissues of Sedum dasyphyllum. Shoot tip explants were grown on Murashige and Skoog (MS) medium. Different concentrations of N6-benzyladenine (BA) or thidiazuron (TDZ) alone or in combination with α-naphthaleneacetic acid (NAA) were tested in order to stimulate multiple shoot production. Ideal shoot induction (100%) and maximized shoot numbers (36.4) were obtained on explants cultured on media incorporated with 2 µM BA and 1 µM NAA combinations. The in vitro-developed shoots rooted best on half-strength MS media incorporated with 2 µM indole 3-butyric acid. Plantlets were effectively acclimatized in the greenhouse with 100% survival rate. The composition and contents of bioactive compounds such as carotenoids, tocopherol and fatty acids in shoot tissues of S. dasyphyllum were investigated using HPLC and GC-MS. The most abundant carotenoid in the shoot tissue was all-E-lutein (40.3-70.5 µg g-1 FW) followed by 9'-Z-neoxanthin (5.3-9.9 µg g-1 FW), all-E-violaxanthin (4.4-8.2 µg g-1 FW), and all-E-β-carotene (1.6-3.6 µg g-1 FW). The α-tocopherol contents of in vitro-raised shoots was 6.5-fold higher than shoots of greenhouse-grown plants. The primary fatty acids found in shoot tissues were α-linolenic acid (32.0-39.3%), linoleic acid (27.4-38.2%), palmitic acid (13.3-15.5%) and stearic acid (5.2-12.2%). In all, summarizing the findings, the micropropagated S. dasyphyllum showed significant enrichment of valuable bioactive carotenoids (92.3 µg g-1 FW), tocopherols (14.6 µg g-1 FW) and α-linolenic acid (39.3%) compared to their greenhouse counterparts. The protocol demonstrated here could be applied for the mass propagation and production of enhanced bioactive compounds from S. dasyphyllum with credibility.

  18. Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale.

    Science.gov (United States)

    Ozel, Cigdem Alev; Khawar, Khalid Mahmood; Unal, Fatma

    2015-03-01

    Endemic Muscari muscarimi Medikus is the most fragrant plant among Muscari species and has a high ornamental potential. The natural populations of M. muscarimi, are severely affected by increased environmental pollution and urbanization. There is a need to develop a micropropagation method that should serve effectively for commercial propagation and conservation. Therefore, the study targeted to set up a strategy for efficient in vitro bulblet regeneration system of M. muscarimi using twin scale bulb explants on 1.0 × MS medium containing 4.44, 8.88, 17.76 μM BAP (6-Benzylaminopurine) plus 2.685, 5.37, 10.74 μM NAA (α-Naphthalene acetic acid). Maximum number of 19 daughter axillary bulblets and 16 daughter adventitious bulblets per twin bulb scale explant was regenerated on 1.0 × MS medium containing 17.76 μM BAP plus 10.74 μM NAA and 17.76 μM BAP plus 2.685 μM NAA respectively. The daughter bulblets regenerated on twin bulb scales on 8 out of 9 regeneration treatment could be easily rooted on 1.0 × MS medium containing 4.9 μM IBA (Indole-3-butyric acid). The daughter bulblets regenerated on 9th treatment (1.0 × MS medium containing 17.76 μM BAP plus 10.74 μM NAA) were transferred to 1.0 × MS medium containing 30 g/l sucrose to break negative carry over effect of this dose of BAP-NAA, where they grew 2-3 roots of variable length. Daughter bulblet diameter was increased by culturing them on 1.0 × MS medium containing 4.44 μM BAP plus 5.37 μM NAA. The results verified that both age and the source of explants had significant effect on regeneration. In another set of experiments, twin scales were obtained from in vitro regenerated daughter bulblets, although they induced bulblets, yet their bulblet regeneration percentage, mean number of bulblets per explant and their diameter were significantly reduced. In vitro regenerated bulblets were acclimatized in growth chamber under ambient conditions of temperature and humidity on

  19. A note on notes: note taking and containment.

    Science.gov (United States)

    Levine, Howard B

    2007-07-01

    In extreme situations of massive projective identification, both the analyst and the patient may come to share a fantasy or belief that his or her own psychic reality will be annihilated if the psychic reality of the other is accepted or adopted (Britton 1998). In the example of' Dr. M and his patient, the paradoxical dilemma around note taking had highly specific transference meanings; it was not simply an instance of the generalized human response of distracted attention that Freud (1912) had spoken of, nor was it the destabilization of analytic functioning that I tried to describe in my work with Mr. L. Whether such meanings will always exist in these situations remains a matter to be determined by further clinical experience. In reopening a dialogue about note taking during sessions, I have attempted to move the discussion away from categorical injunctions about what analysis should or should not do, and instead to foster a more nuanced, dynamic, and pair-specific consideration of the analyst's functioning in the immediate context of the analytic relationship. There is, of course, a wide variety of listening styles among analysts, and each analyst's mental functioning may be affected differently by each patient whom the analyst sees. I have raised many questions in the hopes of stimulating an expanded discussion that will allow us to share our experiences and perhaps reach additional conclusions. Further consideration may lead us to decide whether note taking may have very different meanings for other analysts and analyst-patient pairs, and whether it may serve useful functions in addition to the one that I have described.

  20. Research

    African Journals Online (AJOL)

    abp

    2014-01-31

    Jan 31, 2014 ... by Hazarika in a population-based study in India. The researcher noted that patients' preference to the private health facilities was due mainly to their dissatisfaction with the services in the public health facilities [11]. Furthermore, the quality of the services in the private health facilities could also be a major ...

  1. Micropropagation of transgenic lettuce containing HBsAg as a method of mass-scale production of standardised plant material for biofarming purposes.

    Science.gov (United States)

    Pniewski, Tomasz; Czyż, Marcin; Wyrwa, Katarzyna; Bociąg, Piotr; Krajewski, Paweł; Kapusta, Józef

    2017-01-01

    Micropropagation protocol of transgenic lettuce bearing S-, M- and L-HBsAg was developed for increased production of uniformised material for oral vaccine preparation. Effective manufacturing of plant-based biopharmaceuticals, including oral vaccines, depends on sufficient content of a protein of interest in the initial material and its efficient conversion into an administrable formulation. However, stable production of plants with a uniformised antigen content is equally important for reproducible processing. This can be provided by micropropagation techniques. Here, we present a protocol for micropropagation of transgenic lettuce lines bearing HBV surface antigens: S-, M- and L-HBsAg. These were multiplied through axillary buds to avoid the risk of somaclonal variation. Micropropagation effectiveness reached 3.5-5.7 per passage, which implies potential production of up to 6600 plant clones within a maximum 5 months. Multiplication and rooting rates were statistically homogenous for most transgenic and control plants. For most lines, more than 90 % of clones obtained via in vitro micropropagation had HBsAg content as high as reference plants directly developed from seeds. Clones were also several times more uniform in HBsAg expression. Variation coefficients of HBsAg content did not exceed 10 % for approximately 40-85 % of clones, or reached a maximum 20 % for 90 % of all clones. Tissue culture did not affect total and leaf biomass yields. Seed production for clones was decreased insignificantly and did not impact progeny condition. Micropropagation facilitates a substantial increase in the production of lettuce plants with high and considerably equalised HBsAg contents. This, together with the previously reported optimisation of plant tissue processing and its long-term stability, constitutes a successive step in manufacturing of a standardised anti-HBV oral vaccine of reliable efficacy.

  2. On that Note...

    Science.gov (United States)

    Stein, Harry

    1988-01-01

    Provides suggestions for note-taking from books, lectures, visual presentations, and laboratory experiments to enhance student knowledge, memory, and length of attention span during instruction. Describes topical and structural outlines, visual mapping, charting, three-column note-taking, and concept mapping. Benefits and application of…

  3. Making Notes, Making Meaning.

    Science.gov (United States)

    Burke, Jim

    2002-01-01

    Introduces notetaking tools used successfully with English-as-a-second-language students and low-achieving high school freshmen. Provides an overview of each tool and explains how students use them to take notes when reading textbooks and articles. Notes these tools and academic habits have helped students succeed in their mainstream academic…

  4. Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers.

    Science.gov (United States)

    Goswami, Komal; Sharma, R; Singh, P K; Singh, Govind

    2013-01-01

    A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l(-1)) or kinetin (0.5 mg l(-1)). BA was recorded to be better than kinetin in terms of number of days taken to bud break. The maximum number of shoots per explant was observed on 0.1 mg l(-1) BA and 0.5 mg l(-1) kinetin. Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (0.1 mg l(-1) each), it increased with increasing concentration of BA up to 10.0 mg l(-1). None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation. In the case of a combination of BA + kinetin + IBA, the maximum (5.5 shoots per explants) proliferation was observed on MS medium containing 1.0 mg l(-1) BA + 0.5 mg l(-1) kinetin + 0.5 mg l(-1) IBA or 0.25 mg l(-1) BA + 1.0 mg l(-1) kinetin + 1.0 mg l(-1) IBA. Regenerated shoots showed root induction on MS basal medium or on MS medium containing 1.0 mg l(-1) IBA. It is concluded that a five-fold increase (1.0 mg l(-1) BA + 0.5 mg l(-1) kinetin + 0.5 mg l(-1) IBA) in axillary shoot proliferation, while seven-fold increase (0.25 mg/l mg l(-1) BA + 1.0 mg l(-1) kinetin + 1.0 mg l(-1) IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations. PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication.

  5. A note on Fukui’s note

    Directory of Open Access Journals (Sweden)

    Tsai Cheng-Yu Edwin

    2015-12-01

    Full Text Available This commentary relates Fukui’s (2015 note on weak vs. strong generation to two aspects of quantification in Chinese: quantifier scope and the syntactic licensing conditions of noninterrogative wh-expressions. It is shown that the phenomena under discussion echo Fukui’s (2015 view that only strong generation allows for a deeper understanding of natural language and that dependencies are to be distinguished structurally.

  6. Micropropagation of Capparis decidua (Forsk.) Edgew. - a tree of arid horticulture.

    Science.gov (United States)

    Deora, N S; Shekhawat, N S

    1995-12-01

    A method for micropropagation of mature trees of Capparis decidua was developed. Multiple shoots were obtained from nodal explants on Murashige and Skoog's (1962) medium+0.1mgl(-1) NAA+5.0mgl(-1)BAP+additives (50mgl(-1) ascorbic acid and25 mgl(-1) each of adenine sulphate, L-arginine and citric acid) at 28 ± 2°C, 12 h/dphotoperiod and 35-40 μmol m(-2)s(-1) photon flux density. The shoots were multiplied by (i) subculture of nodal shoot segments onto MS +0.1 mgl(--1) IAA+1.0mgl(-1) BAPH+additives, and (ii) repeated transfer of original explant onto MS+ 0.1mgl(-1) IAA+mg l(-1) BAP+additives, at intervals of 3 weeks. Sixty to 70% of the shoots rooted when pulse treated with 100 mg l(-1) IBA in half strength MS liquid medium for 4h, and then transferred onto hormone-free half-strength agar-gelled MS basal saltmedium. Incubation in dark at 33 ± 2°C for 6d favoured root induction. In vitro hardened plants were transferred to pots.

  7. Meristem micropropagation of cassava (Manihot esculenta evokes genome-wide changes in DNA methylation

    Directory of Open Access Journals (Sweden)

    Shedrack Reuben Kitimu

    2015-08-01

    Full Text Available There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic changes during propagation by meristem culture and by field cuttings in five cassava (Manihot esculenta cultivars. Principal Co-ordinate Analysis of profiles generated by Methylation Sensitive Amplified Polymorphism (MSAP revealed clear divergence between samples taken from field-grown cuttings and those recovered from meristem culture. There was also good separation between the tissues of field samples but this effect was less distinct among the meristem culture materials. Application of methylation-sensitive Genotype By Sequencing (msGBS identified 105 candidate epimarks that distinguish between field cutting and meristem culture samples. Cross referencing the sequences of these epimarks to the draft cassava genome revealed 102 sites associated with genes whose homologues have been implicated in a range of fundamental biological processes including cell differentiation, development, sugar metabolism, DNA methylation, stress response, photosynthesis, and transposon activation. We explore the relevance of these findings for the selection of micropropagation systems for use on this and other crops.

  8. Micropropagation of Codiaeum variegatum (L.) Blume and regeneration induction via adventitious buds and somatic embryogenesis.

    Science.gov (United States)

    Radice, Silvia

    2010-01-01

    Codiaeum variegatum (L) Blume cv. "Corazon de oro" and cv. "Norma" are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots. The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected.Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton. On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too. Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants. On the other hand, leaf-sectioned of C. variegatum cv. Norma does not start somatic embryo differentiation if 1 mg/L TDZ is not added to the MS basal medium. Incipient callus is observed after 30 days of culture, and then, subculture to MS with 1 mg/L BA allows the same process to show on the "Corazon de oro" cultivar. Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free basal medium with activated charcoal. Root induction is possible on basal medium plus 1 mg/L IBA. Plantlets in the greenhouse have variegated leaves true-to-type.

  9. Meristem micropropagation of cassava (Manihot esculenta) evokes genome-wide changes in DNA methylation.

    Science.gov (United States)

    Kitimu, Shedrack R; Taylor, Julian; March, Timothy J; Tairo, Fred; Wilkinson, Mike J; Rodríguez López, Carlos M

    2015-01-01

    There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic changes during propagation by meristem culture and by field cuttings in five cassava (Manihot esculenta) cultivars. Principal Co-ordinate Analysis of profiles generated by methylation-sensitive amplified polymorphism revealed clear divergence between samples taken from field-grown cuttings and those recovered from meristem culture. There was also good separation between the tissues of field samples but this effect was less distinct among the meristem culture materials. Application of methylation-sensitive Genotype by sequencing identified 105 candidate epimarks that distinguish between field cutting and meristem culture samples. Cross referencing the sequences of these epimarks to the draft cassava genome revealed 102 sites associated with genes whose homologs have been implicated in a range of fundamental biological processes including cell differentiation, development, sugar metabolism, DNA methylation, stress response, photosynthesis, and transposon activation. We explore the relevance of these findings for the selection of micropropagation systems for use on this and other crops.

  10. Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

    Directory of Open Access Journals (Sweden)

    Shahril Efzueni Rozali

    2014-01-01

    Full Text Available A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v NaOCl, 70% (v/v ethanol, and 0.3% (w/v HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP, 1.0 mg/L 1-Naphthaleneacetic acid (NAA, 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA compared to indolebutyric acid (IBA. The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

  11. Enhanced micropropagation and tiller formation in sugarcane through pretreatment of explants with thidiazuron (TDZ).

    Science.gov (United States)

    Kumari, Kavita; Lal, Madan; Saxena, Sangeeta

    2017-10-01

    An efficient, simple and commercially applicable protocol for rapid micropropagation of sugarcane has been designed using variety Co 05011. Pretreatment of shoot tip explants with thidiazuron (TDZ) induced high frequency regeneration of shoot cultures with improved multiplication ratio. The highest frequency (80%) of shoot initiation in explants pretreated with 10 mg/l of TDZ was obtained during the study. Maximum 65% shoot cultures could be established from the explants pretreated with TDZ as compared to minimum 40% establishment in explants without pretreatment. The explants pretreated with 10 mg/l of TDZ required minimum 40 days for the establishment of shoot cultures as compared to untreated explants which required 60 days. The highest average number of shoots per culture (19.1) could be obtained from the explants pretreated with 10 mg/l of TDZ, indicating the highest multiplication ratio (1:6). Highest rooting (over 94%) was obtained in shoots regenerated from pretreated explants on ½ strength MS medium containing 5.0 mg/l of NAA and 50 g/l of sucrose within 15 days. Higher number of tillers/clump (15.3) could be counted in plants regenerated from pretreated explants than untreated ones (10.9 tillers/clump) in field condition, three months after transplantation. Molecular analysis using RAPD and DAMD markers suggested that the pretreatment of explants with TDZ did not adversely affect the genetic stability of regenerated plants and maintained high clonal purity.

  12. Effect of explant density and medium culture volumes on cassava micropropagation in Temporal Immersion System

    Directory of Open Access Journals (Sweden)

    Milagros Basail

    2003-04-01

    Full Text Available Due to the need of producing high quality planting material available to cassava growers, it has been necessary to look for alternatives in order to increase the efficiancy of in vitro propagation methods and their automation, such as the use of the Temporal Immersion Systems (RITA®. This work was carried out to increase the multiplication coefficient for cassava mass propagation through out Temporal Immersion Systems. The clone ‘CMC-40’ was used. Different medium volumes per explant, and material density per unit at a given Immersion frequency were tested. The highest results were obtained in the 2.8 multiplication coefficient with 20 ml culture medium volume and 3.2 using a density of 40 explants/flask. When the Temporal Immersion System is used with these results, a more efficient method for cassava micropropagation is established and also higher quality vitroplants for the rooting stage and further acclimatization in field conditions are produced. Key Words: Tissue Culture, liquid culture medium, Manihot esculenta Crantz

  13. Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir.

    Science.gov (United States)

    Khalafalla, Mutasim M; Daffalla, Hussien M; Abdellatef, Eltayb; Agabna, Elsadig; El-Shemy, Hany A

    2011-04-01

    This report describes in vitro micropropagation of Boscia senegalensis, so-called famine foods, that helped the people in Darfur and Kordofan, Sudan survive during the 1984-1985 famine. Four types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1-5 mg/L 6-benzyladenine (BA). The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA, while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L BA. The shoot cluster, when subcultured to its same medium, significantly increased the rate of shoot multiplication by the end of the third subculture. The maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium. In vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete plantlets. B. senegalensis-regenerated plantlets obtained in vitro for the first time, were hardened and 95% survived under greenhouse conditions.

  14. Micropropagation and Biomass Production of True-to-Type Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Modi, Arpan R; Sharma, Vikas; Patil, Ghanshyam; Singh, Amritpal S; Subhash, N; Kumar, Nitish

    2016-01-01

    Here we describe an efficient micropropagation protocol for Stevia rebaudiana Bertoni. We present experiments carried out to optimize the suitable media for in vitro shoot multiplication and root induction and to study the effect of culture vessel on shoot multiplication. Among all different media tested for in vitro shoot multiplication, hormone-free liquid medium is most suitable. The highest number of nodes per shoot (5.4) and length of shoot (4.76 cm) at 4 weeks after subculturing are observed when single node explants are placed on modified MS medium supplemented with 1 % sucrose and 0.7 % agar. The highest response of multiplication rate (9.56) is observed on half strength of macroelement of MS with full strength of microelement of MS and 170 mg/l KH2PO4, and 185 mg/l MgSO4 in plastic growth container. Further, RAPD marker analysis of in vitro-raised plants maintained their clonal fidelity and true-to-type without showing any somaclonal variation.

  15. Micropropagation and hairy root culture of Ophiorrhiza alata Craib for camptothecin production.

    Science.gov (United States)

    Ya-ut, Pornwilai; Chareonsap, Piyarat; Sukrong, Suchada

    2011-12-01

    An efficient system was developed for the in vitro micropropagation and hairy root culture of Ophiorrhiza alata Craib for camptothecin (CPT) production. Shoot multiplication on leaf and node explants from germinated seeds of O. alata was successful on half-strength Murashige and Skoog medium supplemented with varying amounts of kinetin and α-naphthaleneacetic acid. Node explants grown in vitro were successfully infected by Agrobacterium rhizogenes TISTR 1450 for the establishment of hairy root culture. The amount of CPT in various parts of O. alata was analyzed by HPLC. The accumulation of CPT in transformed hairy roots was twice that in soil-grown plants (785 ± 52 and 388 ± 32 μg/g dry wt, respectively). In the presence of a polystyrene resin (Diaion HP-20) that absorbed CPT, the CPT content in the culture media increased sevenfold compared with controls (1,036 and 151 μg per 250 ml medium, respectively). These results enable the feasible production of CPT of O. alata by means of a cell culture strategy. These measures can help safeguard the plant from extinction. © Springer Science+Business Media B.V. 2011

  16. Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

    Science.gov (United States)

    Rozali, Shahril Efzueni; Rashid, Kamaludin A; Taha, Rosna Mat

    2014-01-01

    A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

  17. Control of media browning in micropropagation of guava (Psidium guajava L.)

    International Nuclear Information System (INIS)

    Ahmad, I.; Nafees, M.; Ashraf, I.

    2016-01-01

    Guava (Psidium guajava L.) is a highly valuable fruit of the tropical regions of the world. This species faces browning or blackening of culture medium during In vitro culture due to leaching of phenolic, microbial contagion and tissue recalcitrance. A study therefore designed to evaluate the effects of antioxidants in reduction of phenolic exudation which hampers In vitro regeneration. The nodal explants of the plant were cultured on MS media after pre-soaking in antioxidant solutions of citric acid, ascorbic acid, poly vinyl pyrrolidine (PVP) and charcoal. After culturing explants, the amount of phenolic exude was determined periodically on spectrophotometer at 750 nm absorbance. Phenolic exudation from guava was significantly reduced in nodes treated with charcoal as compared to control and rest of the treatments. Moreover, guava nodes survival percentage was also significantly increased in charcoal treated nodes. It is concluded that pre-soaking in different antioxidants significantly reduced the media browning and thus micro-propagation of guava could be achieved on commercial basis. (author)

  18. Certain growth related attributes of micropropagated banana under different salinity levels

    International Nuclear Information System (INIS)

    Haq, I.U.; Soomro, F.

    2011-01-01

    The effect of salinity (NaCl) was assessed on banana (Musa spp.) cv., Sindhri Banana (Basrai) propagating plantlets in aseptic condition. Four different NaCl levels [0 (control) 50, 100 and 150 mM] were maintained at shoot multiplication stage for 6-weeks. Salinity reduced the number of plantlets per explants and plant biomass significantly. A proportional relationship was observed for Na/sup +/ and Cl/sub -/ but K/sup +/, Ca/sup 2+/and NO/sub 3/ were observed to be inversely proportioned with NaCl stress. Similarly, total proteins as well as carbohydrate contents were decreased significantly. Increasing mode of secondary metabolites (proline, betaine contents and reducing sugars) were showing a negative relationship of saline stress with plant micro-propagation efficiency. Among photosynthetic pigments, total carotenoids were increased while chlorophyll contents (Chl a and b) decreased. Similarly, nitrate reductase activity also reduced. Overall, vegetative propagation of banana was affected significantly by NaCl stress under in-vitro conditions. (author)

  19. Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae).

    Science.gov (United States)

    Braga, Virgínia F; Mendes, Giselle C; Oliveira, Raphael T R; Soares, Carla Q G; Resende, Cristiano F; Pinto, Leandro C; Santana, Reinaldo de; Viccini, Lyderson F; Raposo, Nádia R B; Peixoto, Paulo H P

    2012-03-01

    This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC(50) = 169.3 µg mL(-1). HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg(-1), which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis.

  20. Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

    Science.gov (United States)

    Watad, A A; Kochba, M; Nissim, A; Gaba, V

    1995-03-01

    An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die.

  1. Micropropagation of ginger (zingiber officinale var. rubrum) using buds from microshoots

    International Nuclear Information System (INIS)

    Zuraida, A.R.

    2016-01-01

    Zingiber officinale var. Rubrum (ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2). (author)

  2. Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

    Science.gov (United States)

    Reis, Ricardo S.; Heringer, Angelo S.; Rangel, Patricia L.; Santa-Catarina, Claudete; Grativol, Clícia; Veiga, Carlos F. M.; Souza-Filho, Gonçalo A.

    2017-01-01

    Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress. PMID:28419154

  3. Micropropagation of Phalaenopsis orchids via protocorms and protocorm-like bodies.

    Science.gov (United States)

    Paek, Kee Yoeup; Hahn, Eun Joo; Park, So Young

    2011-01-01

    Phalaenopsis orchids have high economic value in the floriculture industry. Hybridization or cross-pollination in the breeding program have proven to be very reliable techniques for the production of a wide range of successful cultivars with attractive combinations of spray length, bud number, flower color and type, fragrance, seasonality, and compactness. In vitro propagation makes it possible to clonally mass propagate hybrids of commercial value and conserved species. However, in vitro culture technologies are still a challenge because of the slow growth of plantlets, low multiplication rate, poor rooting, and somaclonal variation. Although seed-raised plants can be used for conservation and breeding for the selection of superior features, genetic characteristics including seasonality, inflorescence, flower color, and type are not uniform. In this regard, micropropagation through protocorm-like bodies obtained from germinating embryos and somatic tissues is an important strategy in obtaining genetically stable plants and the improvement of quality. However, not all genotypes of Phalaenopsis respond to the same protocol under the same culture conditions and often result in the development of undesirable characteristics. In this chapter, plantlet production in Phalaenopsis orchids via the culture of protocorms from seeds and protocorm-like bodies from leaf sections and root tips are detailed.

  4. Comparing the Effects of Benzyladenine and meta-Topolin on Sweet Basil (Ocimum basilicum Micropropagation

    Directory of Open Access Journals (Sweden)

    Szidónia KŐSZEGHI

    2014-12-01

    Full Text Available Micropropagation of aromatic plants reveals an effective way of obtaining high volume, virus-free plant material of uniform quality. The application of meta-Topolin (mT (N6-(2-hydroxybenzyl adenine-9-riboside and aromatic cytokinin as Benzyladenine (BAP in the micro propagation of sweet basil (Ocimum basilicum L. was tested for the first time and plant growth parameters assessed to determine the optimum level of these cytokinins. Additionally, the rate of root-growth inhibition due to these two cytokinins was also assessed. Our results show that 1 mg/l (4.43 mM BAP and 0.5 mg/l (2.07 mMmT produced the most favourable effects on new shoot developments. Meta-Topolin was shown to increase the quality of the plants and in comparison with BAP fewer distortions were observed. No significant differences in root-growth inhibition between the mT and BAP were detected.  

  5. Monitoring the infective process of the downy mildew causal agent within micropropagated rose plants

    Directory of Open Access Journals (Sweden)

    Sonia Yamile Gómez

    2012-08-01

    Full Text Available Downy mildew in the rose caused by a species of the Peronospora genus is a very restrictive disease for the Colombian greenhouse rose production. The damage observed in the susceptible varieties of commercial rose include symptoms affect young steams and tiny leaves causing reddish and brown spots and defoliation; leading to 10% production losses. The infective behavior of this pathogen was studied with the aim of increasing the knowledge about the biology of the rose downy mildew. The study of the infective process was performed on the Charlotte variety using micropropagated roses inoculated with suspensions of sporangia. A germinal tube was observed during the germination process, it came from a lateral papilla and reached up to 300 microns in length. During this study, the ability of the pathogen to use vascular sieves as communication systems within the plant was determined. Oogonia and antheridia were also observed inside the epidermal cells, and oospores inside the parenchymal tissue close to xylem vessels. To the best of our knowledge, these sexual structures have not been reported on in Colombia before. This study verifies the ability of the downy mildew causal agent to move through the xylem vessels and produce sexual structures, such as oogonia, antheridia and oospores within those tissues.

  6. Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants.

    Science.gov (United States)

    Caro, L A; Polci, P A; Lindström, L I; Echenique, C V; Hernández, L F

    2002-04-01

    Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P. chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1-2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successful with cuttings taken from young or adult plants, but explants from young plants showed a better response. Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.

  7. Micropropagation of the new apple rootstock ‘G. 814’

    Directory of Open Access Journals (Sweden)

    Aline Meneguzzi

    Full Text Available ABSTRACT: International breeding programs launched new genetic material of apple rootstocks that in addition to precocity and great yield are resistant to major diseases and soil pests encountered in the largest apple producing regions in Brazil. Given this, there is a necessity for vegetative propagation of these materials for study and possible replacement of existing rootstocks. The objective was to adapt a micropropagation protocol for new apple rootstock ‘G. 814’. In the multiplication phase were evaluated BAP concentrations: 0; 0.5; 1; 2 and 4mg L-1 and in the rooting phase were evaluated IBA concentrations: 0; 0.25; 0.50; 1; 1.5 and 2.5mg L-1. These new results demonstrated that this new rootstock selection can be propagated with this tissue culture adapted protocol. For the successful in vitro propagation of apple rootstock ‘G. 814’ it is indicated the use of 1mg L-1 BAP at multiplication phase and 1.5mg L-1 IBA at rooting phase.

  8. Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

    Directory of Open Access Journals (Sweden)

    Nadjma Souza Leite

    2012-10-01

    Full Text Available This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7. Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL. Imperial cultivar showed higher bromelain activity than the Pérola's.

  9. Micropropagation and protein profile analysis by SDS-PAGE of Gracilaria changii (Rhodophyta, Solieriaceae

    Directory of Open Access Journals (Sweden)

    Lin Wei Jong

    2015-05-01

    Full Text Available Gracilaria changii seaweed is primarily important as a source of agar with wide applications in food industries. The high demand of agar led to gradual depletion of G. changii in natural resources. Establishment of in vitro culture of G. changii has an important role and allowing G. changii explants to grow optimally under controlled conditions to provide constant, continuous and sufficient seedlings supply for Gracilaria farming. This study focused on micropropagation culture of G. changii in which different exogenous factors influencing seaweed growth were investigated: strength of chosen medium Provasoli’s enriched seawater (PES, types and concentration of fertilizers/biostimulant, supplementation of plant growth regulators and seawater salinity. The results were presented in daily growth rate of explants and data analysis was carried out using one-way ANOVA. The results demonstrated high growth rate of G. changii in 25% of PES supplemented with 5 mg L−1 AMPEP, and seawater salinity range between 30 and 40 ppt, respectively. Protein profiles of tissue-cultured and farm cultivated G. changii were produced by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE. The results demonstrated no remarkable difference in the protein profiles and indicated the suitability of the culture condition for the growth of G. changii.

  10. Suicide Notes in Hong Kong in 2000

    Science.gov (United States)

    Wong, Paul W. C.; Yeung, April W. M.; Chan, Wincy S. C.; Yip, Paul S. F.; Tang, Arthur K. H.

    2009-01-01

    Suicide notes have been regarded as one of the most informative data sources to understand the reasons why people commit suicide. However, there is a paucity of suicide note studies, leaving researchers with an assumption that this phenomenon remains static over time. This study examines this assumption by comparing the characteristics of…

  11. NCEP Internal Office Notes

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Centers for Environmental Prediction (NCEP) and its predecessors have produced internal publications, known as Office Notes, since the mid-1950's. In...

  12. Writing a Condolence Note

    Science.gov (United States)

    ... through the eyes of others. For example, a mother whose son had died found out that her ... few suggestions on ending your condolence note: “Our love and support will always be here for you.” “ ...

  13. Analytical dynamics course notes

    CERN Document Server

    Lindenbaum, Samuel D

    1994-01-01

    This book comprises a set of lecture notes on rational mechanics, for part of the graduate physics curriculum, delivered by the late Prof. Shirley L. Quimby during his tenure at Columbia University, New York. The notes contain proofs of basic theorems, derivations of formulae and amplification of observations, as well as the presentation and solution of illustrative problems. Collateral readings from more than 50 source references are indicated at appropriate places in the text.

  14. Note Taking and Note Sharing While Browsing Campaign Information

    DEFF Research Database (Denmark)

    Robertson, Scott P.; Vatrapu, Ravi; Abraham, George

    2009-01-01

    Participants were observed while searching and browsing the internet for campaign information in a mock-voting situation in three online note-taking conditions: No Notes, Private Notes, and Shared Notes. Note taking significantly influenced the manner in which participants browsed for information...

  15. Micropropagação de Cabralea canjerana Micropropagation of Cabralea canjerana

    Directory of Open Access Journals (Sweden)

    Silvana Cruz da Rocha

    2007-02-01

    Full Text Available A Cabralea canjerana (Vell. Mart. (Meliaceae (canjarana é uma espécie arbórea nativa brasileira importante para fornecimento de madeira de boa qualidade. As sementes desta espécie não podem ser armazenadas por muito tempo e, por tanto, existe a necessidade do desenvolvimento de técnicas alternativas de propagação como a micropropagação. Neste trabalho, foram realizados experimentos de multiplicação utilizando segmentos nodais, retirados de plantas germinadas in vitro. Os segmentos foram inoculados em meio de cultura MS ou WPM, adicionado de 6-benzilaminopurina (BAP e, ou, 2-isopenteniladenina (2-iP nas concentrações de 2,5 ou 5 µM. Microestacas de rebrotas foram colocadas em meio de cultura MS/2, com a metade da concentração dos sais do meio MS, adicionado de ácido indol 3-butírico (AIB (0, 2,5 e 5 µM. Após sete dias, foram transferidas para meio MS/2 sem auxina e na luz. Na fase de multiplicação, o meio de cultura MS foi mais adequado que o meio WPM. O segmento nodal, em presença de 2,5 µM de BAP, propiciou um dos melhores resultados, com uma taxa de multiplicação de 1,77 por mês, em meio de cultura MS. O enraizamento das microestacas oriundas de rebrotas foi de 87,5% em presença de 5 µM de AIB durante sete dias. A aclimatização foi realizada em casa de vegetação e proporcionou 90% de sobrevivência das mudas após 30 dias. A micropropagação da canjarana a partir de segmentos nodais de mudas cultivadas in vitro é viável para a multiplicação dessa espécie.Cabralea canjerana (Vell. Mart. (Meliaceae ("canjarana" is a native tree of economic importance in Brazil. The storage of seeds is of short duration and it is therefore necessary to establish a protocol for micropropagation of this species. In this work, multiplication experiments were carried out using nodal segments, excised from in vitro germinated plants. The segments were inoculated in MS or WPM culture medium, supplemented with 6-benzylaminopurine

  16. The influence of fast neutron irradiation through micropropagation, calli induction and cell aggregate suspension culture of tapak dara cultivate vinca rosea linn

    International Nuclear Information System (INIS)

    Wahid, Rosmiarty A.

    2000-01-01

    Study on the influence of fast neutron irradiation toward tissue induction of apical shoot, calli of leaf and corolla as well as development of bud micropropagation using variety of MS and Gamborg (B5) which were supplemented with growth hormone 2,4-D NAA, BAP and kinetin has been carried out. Cell aggregates were obtained from modified liquid media by mixing MS macro element and Gamborg vitamin. Influence of the iow level irradiation (0,5-10 Gy) was investigated for auxiliary bud micropropagation , middle (5 - 20 Gy) for calli induction, while for call aggregates higher doses (until 30 Gy) were used. Optimum growth of bud micropropagation was stimulate at dose range between 0,5-1Gy and grown on MS supplemented whit BAP and NAAN, while for leaf and corolla calli was at 5 Gy, on MS media which was supplemented whit 1 mg/L kinetin, 10mg/L BAP and 0,5 mg/L NAA. However, neutron dose of 10 Gy decreased the induction of leaf and corolla calli. The highest radioresistance was shown by cell aggregates of leaf calli that grew prosperously up to 20Gy. Key words : fast neutron, micropropagation, tissue culture, cell culture, vinca rosea L

  17. Micropropagation of Myriophyllum alterniflorum (Haloragaceae) for stream rehabilitation: first in vitro culture and reintroduction assays of a heavy-metal hyperaccumulator immersed macrophyte.

    Science.gov (United States)

    Delmail, David; Labrousse, Pascal; Hourdin, Philippe; Larcher, Laure; Moesch, Christian; Botineau, Michel

    2013-01-01

    Nowadays, submersed aquatic macrophytes play a key role in stream ecology and they are often used as biomonitors of freshwater quality. So, these plants appear as natural candidates to stream rehabilitation experiments. Among them, the stream macrophyte Myriophyllum alterniflorum is used recently as biomonitor and is potentially useful for the restoration of heavy-metal contaminated localities. The best way to obtain a mass production of watermilfoil plants is micropropagation. We developed in vitro culture of M. alterniflorum and the effects of five media on the plant development were assessed. Five morphological and four physiological endpoints were examined leading to the recommendation of the Murashige and Skoog medium for ecotoxicological studies on chlorophyllous parts, and of the Gaudet medium for root cytotoxicity and phytoremediation studies. Micropropagated clones were acclimatized in a synthetic medium and in situ reintroduction was performed efficiently. This is the first report of micropropagated plants transplantation in streams. The successful establishment of watermilfoil beds even in polluted areas strongly suggested that ecological restoration using micropropagated watermilfoil is a promising biotechnology for phytoremediation and rehabilitation of degraded areas. Moreover, high bioconcentration factors evidenced that watermilfoil hyperaccumulates Cd and Cu, and could be potentially used in phytoremediation studies.

  18. A Micropropagation Protocol for a Critically Endangered Mangrove Excoecaria Agallocha L

    Directory of Open Access Journals (Sweden)

    Panneerselvam RAJARAM

    2012-06-01

    Full Text Available Excoecaria agallocha L. is a critically endangered mangrove tree from the Pichavaram mangrove reserve forest, the Tamil Nadu Coastal area. It is distributed on the seashore and the edge-mangrove. In order to reduce the decrease in number of these Mediterranean mixed stand, unsupervised forest management practices have drastically been reduced. In addition, the deforestation of the mangrove area, along with a low seed germination rate further endanger this species. In this study we developed a protocol for the micropropagation of adult Excoecaria agallocha. Microcuttings were obtained from lateral and apical twigs of mature plants and used as explants. Microcuttings with axillary buds were grown on different media, plant growth regulators and phenolic exudation substances. The axillary shoots produced on uncontaminated explants were excised, segmented and recultured in the same medium, to increase the stock of shoot cultures. The Modified Murashige and Skoog (MMS medium, augmented with different concentrations of N6 – benzyl adenine (BAP and Naphthalene acetic acid (NAA, either alone, or in combinations, as a potential medium for shoot multiplication by nodal segments, was tested. In the following experiment, equal molar concentrations of four cytokonins [BAP, Kinetin and 2- isopenthenyladenine (2iP] in combination with equal molar concentrations of three auxins [ NAA, Indole acetic acid (IAA and indole-3- butyric ]were used to test the rate of axillary shoot proliferation, induced on MMS agar medium supplemented with 3.9 µM BAP and 1.34 µM NAA after 6 weeks in culture. Different auxins (NAA, IBA and IAA were to determine the optimum conditions for in vitro rooting of microshoots. The best results were accomplished with NAA 5.41 µM (89% rooting and with IBA at 2.85 or 5.71µM (86% and 86.5% rooting, respectively.

  19. Adaptive changes in photosynthetic performance and secondary metabolites during white dead nettle micropropagation.

    Science.gov (United States)

    Kapchina-Toteva, V; Dimitrova, M A; Stefanova, M; Koleva, D; Kostov, K; Yordanova, Zh P; Stefanov, D; Zhiponova, M K

    2014-09-15

    The white dead nettle, Lamium album L., is an herb that has been successfully cultivated under in vitro conditions. The L. album micropropagation system offers a combination of factors (light intensity, temperature, carbon dioxide (CO2) level, humidity) that are limiting for plant growth and bioactive capacity. To get a better understanding of the mechanism of plant acclimation towards environmental changes, we performed a comparative investigation on primary and secondary metabolism in fully expanded L. album leaves during the consecutive growth in in situ, in vitro, and ex vitro conditions. Although the genetic identity was not affected, structural and physiological deviations were observed, and the level of bioactive compounds was modified. During in vitro cultivation, the L. album leaves became thinner with unaffected overall leaf organization, but with a reduced number of palisade mesophyll layers. Structural deviation of the thylakoid membrane system was detected. In addition, the photosystem 2 (PS2) electron transport was retarded, and the plants were more vulnerable to light damage as indicated by the decreased photoprotection ability estimated by fluorescence parameters. The related CO2 assimilation and transpiration rates were subsequently reduced, as were the content of essential oils and phenolics. Transfer of the plants ex vitro did not increase the number of palisade numbers, but the chloroplast structure and PS2 functionality were recovered. Strikingly, the rates of CO2 assimilation and transpiration were increased compared to in situ control plants. While the phenolics content reached normal levels during ex vitro growth, the essential oils remained low. Overall, our study broadens the understanding about the nature of plant responses towards environmental conditions. Copyright © 2014 Elsevier GmbH. All rights reserved.

  20. Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants.

    Science.gov (United States)

    Sarmast, Mostafa Khoshhal; Salehi, Hassan; Khosh-Khui, Morteza

    2012-07-01

    The objectives of the present work were in vitro propagation of Araucaria excelsa R. Br. var. glauca Carrière (Norfolk Island pine) with focus on the evaluation of the mean number of shoots per explant (MNS/E) and mean length of shoots per explants (MLS/E) produced by different parts of the orthotropic stem of A. excelsa R. Br. var. glauca in response to plant growth regulators. Norfolk Island pine axillary meristems responded very well to the 2-iso-pentenyl adenine (2iP) and thidiazuron (TDZ) levels. Explants taken from stem upper segments in the media containing 2iP had a higher MNS/E (3.47) and MLS/E (6.27 mm) in comparison to those taken from stem lower segments, which were 0.71 and 0.51 mm, respectively. Using 0.045 μM TDZ in the MS medium not only resulted in 4.60 MNS/E with 7.08 mm MLS/E but proliferated shoots showed a good performance as well. Investigating the best position of stem explant on mother plant as well as the best concentrations of growth regulators were performed which were useful for efficient micropropagation of this plant. Thirty three percent of explants were rooted in the MS medium containing 3 % sucrose, supplemented with 7.5 μM of both NAA and IBA for 2 weeks before transferring to a half strength MS medium without any growth regulator. Plantlets obtained were acclimatized and transferred to the greenhouse with less than 20 % mortality. This procedure considered the first successful report for regeneration and acclimatization of A. excelsa R. Br. var. glauca plantlet through main stem explants.

  1. A regenerative route for Eugenia uniflora L. (Myrtaceae through in vitro germination and micropropagation

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Diniz da Silva

    2014-07-01

    Full Text Available Eugenia uniflora is a tree species native from Central and South America, largely employed in the popular medicine, in the cosmetics and pharmaceutical industries and also consumed in natura. Aiming to provide plant material with high sanity and genetic uniformity for the establishment of commercial plantations, we developed a protocol for seeds disinfestation, in vitro germination and in vitro propagation of this species through organogenesis. Fruits of E. uniflora were obtained from wild trees growing in the Pampa biome, Southern Brazil. Seeds were disinfested using ethanol 70% (10 min and NaOCl 1.25% (10 or 25 min. Shoot apexes and nodal segments of non-contaminated plantlets were cultivated in verification medium AS30 during 20 days, posteriorly in ½MS medium supplemented with sucrose, IBA and BAP during 45 days and acclimatized in greenhouse. Disinfesting seeds with ethanol 70% (10 min and NaOCl 1.25% (25 min allowed germination with significantly lower contamination (2.0% and production of healthy explants for the micropropagation. No difference concerning size and contamination was observed for the propagation using shoot apexes or nodal segments as explant. Acclimatized plants revealed normal phenotype and healthy appearance. This regenerative route can be applied for mass clonal propagation from seeds of cross-pollinated or self-pollinated selected trees aiming the establishment of commercial plantations of E. uniflora and other Myrtaceae species.

  2. BIOMASS ACCUMULATION AND NUTRITION IN MICROPROPAGATED PLANTS OF THE BANANA ‘PRATA CATARINA’ UNDER BIOFERTILISERS

    Directory of Open Access Journals (Sweden)

    EDER DE OLIVEIRA SANTOS

    2017-01-01

    Full Text Available Banana farming is an activity of great economic and social importance, and is carried out in most tropical countries. The aim of this work was to evaluate the biomass accumulation and levels of nitrogen (N, phosphorus (P, potassium (K, calcium (Ca and magnesium (Mg in micropropagated plants of the banana “Prata Catarina” during the acclimatization phase, under different types and doses of biofertilisers. The experimental design included randomised blocks in a 2 × 5 + (2 factorial scheme, with two types of liquid biofertilisers (bovine biofertiliser with anaerobic and aerobic fermentation and five biofertiliser doses (0.25, 0.50, 0.75, 1.00, and 1.25 L plant-1 week-1, as well as two additional treatments (control and recommended mineral fertilisation. The following variables were analysed: dry weight of the leaves and roots, and mineral element content (N, P, K, Ca, and Mg in different parts of the plant (leaf and root. During 90 days of acclimatization, the nutritional contribution of bovine biofertiliser with anaerobic fermentation was greater in comparison with the biofertiliser with aerobic fermentation and the control, but lower in comparison with mineral fertilisation. The 1000-mL dose of the biofertiliser with anaerobic fermentation promoted greater dry weight accumulation in the leaves and roots of the banana “Prata Catarina”. The biofertiliser with anaerobic fermentation promoted higher levels of N, K, and Ca in the leaves, whereas the biofertiliser with aerobic fermentation promoted higher levels of P in the leaves and roots.

  3. Effect of Sucrose and Growth Regulator’s Level on Ginger Micropropagation

    Directory of Open Access Journals (Sweden)

    Resham Babu Amgai

    2017-05-01

    Full Text Available Ginger is most important cash crop of the hilly region of Nepal. However, availability of disease free planting material (rhizome is the major problem faced by Nepalese farmers. Tissue culture is the only option to produce disease free rhizome of ginger. Suitable culture media combination is most important for the production of planting material in ginger through tissue culture. Therefore, effect of different level of sucrose and growth regulators on micro-propagation of ginger was studied using local collection ‘Kaski Local’. Early stage bud was used as explant. MS basal media with different level of sucrose and growth regulators was used as tissue culture media. 30 g/L sucrose, 30 g/L sucrose+5mg/L BA, 30 g/L sucrose+5 mg/L BA+0.5 mg/L NAA, 60 g/L sucrose+5mg/L BA, 60 g/L sucrose+5 mg/L BA+0.5mg/L NAA, 90 g/L sucrose+5 mg/L BA was used in this study. The explants were surface sterilized, cultured and incubated at 25±2°C, 90-95% relative humidity and 14:10 hours light:dark photoperiod for 8 weeks. Increased level of the sucrose increased the rhizome weight, however, addition of NAA produced more positive effect for this. MS basal media with 60 g/L sucrose+5 mg/L BA+0.5 mg/L NAA produced higher rhizome weight.

  4. The Student Movement as an Object of study in the Colombia and continental historiography: Notes for a balance and a research agenda

    Directory of Open Access Journals (Sweden)

    Álvaro Acevedo Tarazona

    2011-07-01

    Argentina and Brazil in order to approach currents and looks beyond the national borders which renew and contextualize our work. In this way, it seeks to help in the construction of a historiography map to locate the new generations of researchers interested in the student movement in order to consolidate an area of research and relection at the national level.

  5. Whose Side Are We on and for Whom Do We Write? Notes on Issues and Challenges Facing Those Researching and Evaluating Public Policy

    Science.gov (United States)

    Warren, Jon; Garthwaite, Kayleigh

    2015-01-01

    Becker (1967) poses the question "Whose side are we on?," a question which has become an enduring part of discussions within social scientific methodology. This paper explores the key issues in Becker's argument and considers its relevance to researchers today, locating this within a consideration of evaluation-based research and policy.…

  6. Grouping Notes Through Nodes

    DEFF Research Database (Denmark)

    Dove, Graham; Abildgaard, Sille Julie Jøhnk; Biskjær, Michael Mose

    , both individually and when grouped, and their role in categorisation in semantic long-term memory. To do this, we adopt a multimodal analytical approach focusing on interaction between humans, and between humans and artefacts, alongside language. We discuss in detail examples of four different...... externalisation functions served by Post-ItTM notes, and show how these functions are present in complex overlapping combinations rather than being discrete. We then show how the temporal development of Post-ItTM note interactions supports categorisation qualities of semantic long-term memory....

  7. Grouping Notes Through Nodes

    DEFF Research Database (Denmark)

    Dove, Graham; Abildgaard, Sille Julie; Biskjær, Michael Mose

    2017-01-01

    , both individually and when grouped, and their role in categorisation in semantic long-term memory. To do this, we adopt a multimodal analytical approach focusing on interaction between humans, and between humans and artefacts, alongside language. We discuss in detail examples of four different...... externalisation functions served by Post-ItTM notes, and show how these functions are present in complex overlapping combinations rather than being discrete. We then show how the temporal development of Post-ItTM note interactions supports categorisation qualities of semantic long-term memory....

  8. Writing a technical note.

    Science.gov (United States)

    Ng, K H; Peh, W C G

    2010-02-01

    A technical note is a short article giving a brief description of a specific development, technique or procedure, or it may describe a modification of an existing technique, procedure or device applicable to medicine. The technique, procedure or device described should have practical value and should contribute to clinical diagnosis or management. It could also present a software tool, or an experimental or computational method. Technical notes are variously referred to as technical innovations or technical developments. The main criteria for publication will be the novelty of concepts involved, the validity of the technique and its potential for clinical applications.

  9. Notes on Piezoelectricity

    Energy Technology Data Exchange (ETDEWEB)

    Redondo, Antonio [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-02-03

    These notes provide a pedagogical discussion of the physics of piezoelectricity. The exposition starts with a brief analysis of the classical (continuum) theory of piezoelectric phenomena in solids. The main subject of the notes is, however, a quantum mechanical analysis. We first derive the Frohlich Hamiltonian as part of the description of the electron-phonon interaction. The results of this analysis are then employed to derive the equations of piezoelectricity. A couple of examples with the zinc blende and and wurtzite structures are presented at the end

  10. Field and laboratory notes on development of a PIT-tag system for spillways - Research and Development of New Marking and Monitoring Technologies

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project addresses how to expand the current fish-tracking technologies to enable the fisheries community to successfully carry out the actions, research, and...

  11. Research Note: Equivalence of French and English Language Versions of the Center for Epidemiologic Studies-Depression Scale (CES-D) among Caregivers of Persons with Dementia

    Science.gov (United States)

    O'Rourke, Norm

    2003-01-01

    The Center for Epidemiologic Studies-Depression Scale (CES-D) is among the most widely used depression screening measures. Existing research suggests a higher-order factor structure of responses among older adults (factors labelled "depressive affect," "absence of well-being," "somatic symptoms," and "interpersonal affect," each loading upon a…

  12. The Student Movement as an Object of study in the Colombia and continental historiography: Notes for a balance and a research agenda

    OpenAIRE

    Álvaro Acevedo Tarazona; Gabriel Samacá Alonso

    2011-01-01

    The events of 1968 are recognized today as the irst global Cultural Revolution. Latin America was not stranger to the big changes in a time known for cultural social movements and student protests. Today is not yet known a balance about the Continental Historiography of this event. This article attempts to point out the limitations of scientiic research in Colombia, marked by their fragmented and dispersed character. In a continental level are explored the cases of Mexico, Argentina and Br...

  13. Note Taking for Geography Students.

    Science.gov (United States)

    Kneale, Pauline E.

    1998-01-01

    Addresses geography students' questions about why, when, and how to take notes. Outlines a step-by-step process for taking notes from written sources and from class lectures. Discusses what types of notes are appropriate for various types of sources. Suggests some ideas for making notes useful for individual learning styles. (DSK)

  14. Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae

    Directory of Open Access Journals (Sweden)

    Virgínia F. Braga

    2012-03-01

    Full Text Available This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM/α-naphthaleneacetic acid (NAA; 0.005 µM induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM. Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg-1, which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis.Este trabalho descreve um protocolo eficiente de micropropagação para Verbena litoralis e estuda as atividades antinociceptiva e antioxidante de extratos desta espécie. Para o estabelecimento in vitro, os procedimentos de desinfecção e o PVPP mostraram alta eficiência no controle da contaminação por fungos e bactérias e da oxidação fenólica. O cultivo de segmentos nodais em meio MS suplementado com 6-benziladenina (7,5 µM e ácido α-naftalenoacético (ANA; 0,005 µM induziu múltiplos brotos. Brotos alongados foram enraizados com AIA (0,2 µM. As taxas de aclimatização foram elevadas e as plantas apresentaram características típicas da espécie. A fração hexânica (FH de folhas trituradas apresentou atividade sequestradora de radicais livres com IC50 = 169,3 µg mL-1. A FH mostrou atividade analgésica não dose-dependente no teste das contor

  15. Biosphere-Atmosphere Transfer Scheme (BATS) version le as coupled to the NCAR community climate model. Technical note. [NCAR (National Center for Atmospheric Research)

    Energy Technology Data Exchange (ETDEWEB)

    Dickinson, R.E.; Henderson-Sellers, A.; Kennedy, P.J.

    1993-08-01

    A comprehensive model of land-surface processes has been under development suitable for use with various National Center for Atmospheric Research (NCAR) General Circulation Models (GCMs). Special emphasis has been given to describing properly the role of vegetation in modifying the surface moisture and energy budgets. The result of these efforts has been incorporated into a boundary package, referred to as the Biosphere-Atmosphere Transfer Scheme (BATS). The current frozen version, BATS1e is a piece of software about four thousand lines of code that runs as an offline version or coupled to the Community Climate Model (CCM).

  16. Suicide note themes and suicide prevention.

    Science.gov (United States)

    Foster, Tom

    2003-01-01

    The aim was to determine if suicide note themes might inform suicide prevention strategies. The themes of 42 suicide notes from the Northern Ireland Suicide Study (major psychological autopsy study) were examined. The commonest themes were "apology/shame" (74%), "love for those left behind" (60%), "life too much to bear" (48%), "instructions regarding practical affairs post-mortem" (36%), "hopelessness/nothing to live for" (21%) and "advice for those left behind" (21%). Notes of suicides with major unipolar depression were more likely than notes of suicides without major unipolar depression to contain the themes "instructions regarding practical affairs post-mortem" (67% versus 19%, p = 0.005) and "hopelessness/nothing to live for" (40% versus 11%, p = 0.049). Notes of suicides with a previous history of deliberate self-harm were less likely than notes of suicides without a history of deliberate self-harm to contain the theme "apology/shame" (58% versus 87%, p = 0.04). Notes of elderly suicides were more likely than non-elderly notes to contain the theme "burden to others" (40% versus 3%, p = 0.03). The fact that three quarters of suicide notes contained the theme "apology/shame" suggests that the deceased may have welcomed alternative solutions for their predicaments. Scrutiny of suicide note themes in the light of previous research findings suggests that cognitive therapy techniques, especially problem solving, may have an important role to play in suicide prevention and that potential major unipolar depressive (possibly less impulsive) suicides, in particular, may provide fertile ground for therapeutic intervention (physical and psychological). Ideally all primary care doctors and mental health professionals working with (potentially) suicidal people should be familiar with basic cognitive therapy techniques, especially problem solving skills training.

  17. A note on Marx

    OpenAIRE

    Olesen, Finn; Jensen, Frank

    2001-01-01

    Throughout all his life Karl Marx wrote angrily about capitalism. By use of a dialectic approach he was convinced that the working class had to unite and make a social revolution and thereby free them selves from exploitation. Marx himself was in many ways a dialectic person as we try to show in the note. So in some sense he became one with his scientific methodology.

  18. Insights regarding the Usefulness of Partial Notes in Mathematics Courses

    Science.gov (United States)

    Cardetti, Fabiana; Khamsemanan, Nirattaya; Orgnero, M. Carolina

    2010-01-01

    Note-taking is a widespread practice used by college students to record information from lectures. Unfortunately, even successful students' notes are incomplete and, therefore, may lack the potential to positively impact their academic performance. Research suggests that instructors can help students improve their note-taking skills by using…

  19. The effects of reinforcement and response-cost on a delayed response task in children with attention deficit hyperactivity disorder: a research note.

    Science.gov (United States)

    Solanto, M V

    1990-07-01

    Children with attention deficit hyperactivity disorder are more inattentive, active, and impulsive than normal children. Some researchers have postulated that these symptoms can all be explained as a result of reduced sensitivity to reinforcement. In order to evaluate this hypothesis, we tested 20 ADD-H children and 18 matched normal controls, 4 1/2-11 years of age, on a delayed response task, a measure of impulsiveness, under conditions of positive reinforcement, and punishment in the form of response-cost. The contingencies each improved performance compared to baseline but did not differ significantly from each other. Neither contingency affected the groups differentially, thus failing to provide support for the reinforcement hypothesis.

  20. A hybrid clinical-research depth electrode for acute and chronic in vivo microelectrode recording of human brain neurons. Technical note.

    Science.gov (United States)

    Howard, M A; Volkov, I O; Granner, M A; Damasio, H M; Ollendieck, M C; Bakken, H E

    1996-01-01

    For several decades, important scientific information has been gained from in vivo microelectrode recordings of individual human cerebral cortical neurons in patients with epilepsy. The experimental methods used, however, are technically complex and require a highly skilled intraoperative team. There are also significant experimental time limitations, as well as constraints on the type of behavioral tests conducted, and the brain regions that may be safely studied. In this report, a new method is described for obtaining in vivo microelectrode recordings using a hybrid depth electrode (HDE). High-impedance research recording contacts are interspersed between low-impedance clinical electroencephalographic (EEG) contacts along the HDE shaft. The HDE has the same external physical properties as a standard clinical depth electrode (DE). Following preclinical laboratory testing, 15 HDEs were used in the evaluation of six patients with medically refractory epilepsy. High-quality EEG recordings were obtained in all cases (two acute intraoperative, four from the chronic epilepsy monitoring unit). Action potentials from individual neurons were successfully recorded during all experimental sessions; however, the chronic preparations were clearly superior. Chronic HDEs are placed using a standard stereotactic system, and the locations of recording contacts are documented on a postimplantation imaging study. The quality of the chronic research recordings was excellent over study periods ranging from 5 to 14 days. The patients rested comfortably on the ward and were able to cooperate with complex experimental instructions. Basic neuroscientists participated fully in all aspects of the chronic investigations. The use of an HDE in place of a standard clinical DE may now allow detailed physiological investigations of any brain region targeted for clinical DE implantation.

  1. Effects of Note-Taking Instruction and Note-Taking Languages on College EFL Students' Listening Comprehension

    Science.gov (United States)

    Tsai-Fu, Tsai; Wu, Yongan

    2010-01-01

    Background: The effect of note-taking has been well-recognized by EFL educators. However, little empirical research has been done to investigate combined effects of note-taking instruction and note-taking language (whether in L1 or L2) in an acquisition-poor environment, where English is used as an instructional language yet the audience is…

  2. Mining Contratação License in the New Regulatory Framework of Brazilian Mining: Some Notes on the Institutes of Research Authorization and Mining Concession

    Directory of Open Access Journals (Sweden)

    Adhemar Ronquim Filho

    2013-06-01

    Full Text Available Given the importance of mining nowadays, Government seeks ways to stimulate its growth, focusing on potentializing research and the advancement of mineral processing, the basic items for speeding up this activity in a profitable way. In this sense, the discussions on the crystallization of a new regulatory framework for the Brazilian mining have been deepened and, despite gathering a significant number of proposals, it does not have a closed text, and, currently, it is far from obtaining an approval or a final word (despite the urgency. However, the analysis of the proposals that have been presented reveals that there is an intention to institute new rules for the modernization of Brazilian mining, and this paper has the purpose of suggesting ways to reconcile conflicts permeated by various dissonant interests that surround the Brazilian mining at this time. It should be emphasized that, given the lack of official disclosure of the amendments proposed, the approach will continue limited to what has been released by MME (Ministry of Mines and Energy and by the studies that have already been presented by experts in the field (connected to government and/or private businesses. It is restricted to discuss changes to be implemented with the new regulatory framework, highlighting points to be observed, and, among the topics that require mandatory update, we can emphasize the changes in the procedures of exploration permits and mining.

  3. Towards an energy transition? Which energies for tomorrow and for all, on the territory. Research-action, problematic note - Activity report 2012-2014 - Final report. Citizen recommendations

    International Nuclear Information System (INIS)

    Aubin, Samuel; Lemoult, Bernard; Allagnat, Bernard; Cohu, Sylvie; Gadoin, Emilie; Crochet, Moise; Dothee, Jean-Luc; Gauduchon, Marie-Veronique; Gendre, Nicolas; Musard, Denis; Retiere, Alain; Vacher, Pierre; Vilbert, Christine; Petillon, Xavier; Damerose, Clotilde

    2014-11-01

    This document first presents a project launched on the issue of energy choice for tomorrow for the territory, and more particularly on a shared regional diagnosis and principles for action. A second document reports activities of this research-action project which aimed at making citizen recommendations emerge, and at elaborating an operational territorial tool for energy transition. Then comes the final report of this project. After an introduction which outlines that energy transition is a major stake for the territory, a first chapter discusses which are tomorrow's energies by outlining the climatic emergency, the energy situation at the world level, the perspective of smart cities and communities, national energetic stakes, the role given to citizen, and the perspective of a new relationship to the world and to the nature. The second chapter briefly evokes the perspective for 2050. The next part discusses the societal dimension of energy transition, and examines what could be the engines for change. The next chapter identifies and discusses various debates to be held: on climate situation, on energy resource availability, on human development and on the economic model. The last chapter outlines the role of the territorial scale and of collective dynamics to elaborate and implement scenarios of energy transition

  4. Plant germination and production of callus from the yellow hornpoppy (Glaucium flavum): the first stage of micropropagation.

    Science.gov (United States)

    Mohamed, M E; Arafa, A M; Soliman, S S; Eldahmy, S I

    2014-09-01

    The yellow hornpoppy, Glaucium flavum Cr. (Fam. Papaveraceae) is a perennial herb, distributed in the Mediterranean region, including Egypt. The plant contains many benzyl isoquinoline alkaloids from the aporphine type such as glaucine, isoboldine, 1-chelidonine, 1-norchelidonine and 3-O-methylarterenol, making it to display various medicinal activities including antitussive, anticancer, antioxidant, antimicrobial, antiviral, hypoglycemic, analgesic, antipyretic, bronchodilator and anti-inflammatory effects. The plant is now rare and endangered in the Egyptian flora due to urban sprawl. The present study looks into Glaucium flavum seeds' in vitro germination as well as the ability of the explants taken from the growing seedlings to form stable callus lines in order to enable micropropagation as a way to save the rare plant. The study also scans the production of different medicinally valuable alkaloids, particularly glaucine, in produced callus.

  5. A simple and cost effective liquid culture system for the micropropagation of two commercially important apple rootstocks.

    Science.gov (United States)

    Mehta, Mohina; Ram, Raja; Bhattacharya, Amita

    2014-07-01

    The two commercially important apple rootstocks i.e., MM106 and B9 were micropropagated using a liquid culture system. Three different strengths of 0.8% agar solidified PGR free basal MS medium were first tested to optimize the culture media for both the rootstocks. Full strength medium (MS0) supported maximum in vitro growth, multiplication, rooting and survival under field conditions as opposed to quarter and half strength media. When three different volumes of liquid MS0 were tested, highest in vitro growth, multiplication, rooting and also survival under field conditions were achieved in 20 mL liquid MS0. The cost of one litre of liquid medium was also reduced by 8 times to Rs. 6.29 as compared to solid medium. The cost of 20 mL medium was further reduced to Rs. 0.125.

  6. Effect of Different Concentrations of Growth Regulators on Gardenia jasminoides cv. Veitchii Micropropagation by Tissue Culture Technique

    Directory of Open Access Journals (Sweden)

    G. R. Abdullah

    2003-01-01

    Full Text Available Micropropagation techniques were set up for Gardenia jasminoides c.v. veitchi. Many plantlets were obtained by culturing shoot cuttings in MS nutrient media, 30 g/L Sucrose, 7 g/L Agar Agar, and different concentrations of BAP and IAA. The best concentration was 1mg /L BAP with 0.5 mg/L IAA. This concentration gave the best sprout growth suitable for rooting in primary and secondary culture by reculturing the stuck cutting every 6 weeks and for many times. We also obtained a high rooting percentage up to 98 % of natural rooting in rooting media different from propagation media by reducing mineral salt concentration to half, Sucrose to 20gm/L, and 2gm/L active charcoal, and 1mg/L IAA. Plantlets were transferred to greenhous and subjected for hardening. This technique gave 22 plantlets from one cutting in one year.

  7. Effects of growth regulators and activated charcoal for the micropropagation improvement of Dendrobium friedericksianum Rchb.f.

    Directory of Open Access Journals (Sweden)

    Prasertsongskun, S. and

    2004-09-01

    Full Text Available The effects of different concentrations of growth regulators and activated charcoal on micropropagation were studied. Seedlings of orchid, Dendrobium friedericksianum Rchb.f, cultured on Vacin and Went medium containing 1.0 mg/l α-naphthaleneacetic acid (NAA and 1.0 mg/l kinetin (KN significantly yielded per cultured plant, the highest average number of leaves (4.40, roots (8.30, shoot length (1.67 centimeters and fresh weight (0.23 grams. Particularly when activated charcoal was added (0.3% w/v the average number of leaves, roots, shoot length and fresh weight per cultured plant increased. Percentage survival of the plantlet after 21 days of transplantation was 85%.

  8. Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action

    International Nuclear Information System (INIS)

    Žiauka, Jonas; Kuusienė, Sigutė; Šilininkas, Mindaugas

    2013-01-01

    Representatives of the genus Populus (poplars), such as Populus tremula L. (European aspen) and its fast-growing hybrids, are recognized as being among the most suitable tree species for short rotation coppicing in Northern Europe. Several technologies have been developed for fast propagation of selected aspen genotypes, including laboratory (in vitro) micropropagation, which is usually based on the action of exogenous plant hormones. Seeking to minimize the use of the latter, the present study was designed to test if the conditions suitable for increased accumulation of plant-produced gas, including the gaseous plant hormone ethylene, inside a culture vessel could contribute to commercially desirable changes in aspen development. Shoot cultures of several European and hybrid (Populus tremuloides Michx. × P. tremula) aspen genotypes were studied using two different types of culture vessels: tightly sealed Petri dishes (15 × 54 mm) designed to provide restricted gas exchange (RGE) conditions, and capped (but not sealed) test tubes (150 × 18 mm) providing control conditions. Under RGE conditions, not only the positive impact of the ethylene precursors 1-aminocyclopropane-1-carboxylic-acid (ACC) and ethephon on shoot proliferation was demonstrated but also a several-fold increase, compared to the control conditions, in the mean shoot number per explant was recorded even on the hormone-free nutrient medium. Moreover, the shoots developed under RGE conditions were distinguished by superior rooting ability in the subsequent culture. These results suggest that a plant micropropagation system based on the action of plant-produced ethylene rather than of exogenous hormones is possible. -- Highlights: ► Aspen in vitro cultures were grown in different vessels. ► Small-volume vessels were used for restriction of gas exchange. ► Aspen explants produced most shoots in small-volume vessels. ► Shoot proliferation was increased due to explant response to ethylene.

  9. Micropropagation and validation of genetic and biochemical fidelity amongst regenerants of Cassia angustifolia Vahl employing RAPD marker and HPLC.

    Science.gov (United States)

    Chetri, Siva K; Sardar, Pratima Rani; Agrawal, Veena

    2014-10-01

    In vitro protocol has been established for clonal propagation of Cassia angustifolia Vahl which is an important source of anticancerous bioactive compounds, sennoside A and B. Nodal explants excised from field raised elite plant (showing optimum level of sennoside A and B) of C. angustifolia when reared on Murashige and Skoog's medium augmented with different cytokinins, viz. N(6)-benzyladenine (BA), N(6)-(2-isopentenyl) adenine (2iP) and 6-furfuryl aminopurine (Kn) differentiated multiple shoots in their axils. Of the three cytokinins, BA at 5 μM proved optimum for differentiating multiple shoots in 95 % cultures with an average of 9.14 shoots per explant within 8 weeks of culture. Nearly, 95 % of the excised in vitro shoots rooted on half strength MS medium supplemented with 10 μM indole-3-butyric acid (IBA). The phenotypically similar micropropagated plants were evaluated for their genetic fidelity employing random amplified polymorphic DNA (RAPD) markers. Eleven individuals, randomly chosen amongst a population of 120 regenerants were compared with the donor plant. A total of 36 scorable bands, ranging in size from 100 to 1,000 bp were generated amongst them by the RAPD primers. All banding profiles from micropropagated plants were monomorphic and similar to those of mother plant proving their true to the type nature. Besides, high performance liquid chromatography evaluation of the sennoside A and B content amongst leaves of the mature regenerants and the elite mother plant too revealed consistency in their content.

  10. Micropropagation and validation of genetic and biochemical fidelity among regenerants of Nothapodytes nimmoniana (Graham) Mabb. employing ISSR markers and HPLC.

    Science.gov (United States)

    Prakash, Lokesh; Middha, Sushil Kumar; Mohanty, Sudipta Kumar; Swamy, Mallappa Kumara

    2016-12-01

    An in vitro protocol has been established for clonal propagation of Nothapodytes nimmoniana which is an important source of Camptothecin (CPT). Elite source was identified based on the chemical potency to accumulate the optimum level of CPT. Different types and concentrations of plant growth regulators were used to study their effect on inducing multiple shoots from the explants regenerated from embryos of N. nimmoniana. Of these, a combination of N6-benzyladenine (0.2 mg L -1 ) and Indole-3-butyric acid (IBA) (0.1 mg L -1 ) proved optimum for differentiating multiple shoots in 90.6 % of the cultures with an average of 10.24 shoots per explant obtained within 8 weeks of inoculation. Nearly, 92 % of the excised in vitro shoots rooted on half strength Murashige and Skoog (MS) medium containing 0.05 % activated charcoal, supplemented with 1-naphthaleneacetic acid and IBA at 0.1 mg L -1 each. The micropropagated plants were evaluated for their genetic fidelity by employing inter simple sequence repeats (ISSR) markers. Ten individuals, randomly chosen from a population of 145 regenerants, were compared with the donor plant. The regenerated plants were also evaluated for their chemical potency using high-performance liquid chromatography (HPLC) analysis of CPT content. The true-to-type nature of the micropropagated plants was confirmed based on their monomorphic banding profiles with that of the mother plants using ISSR markers. Besides, HPLC evaluation of the CPT content confirmed the existence of chemical uniformity among the regenerated plants and the elite mother plant.

  11. Preculturing effect of thidiazuron on in vitro shoot multiplication and micropropagation round in Capparis decidua (Forsk.) an important multipurpose plant.

    Science.gov (United States)

    Bukhari, Najat A W; Siddique, Iram; Perveen, Kahkashan

    2016-09-01

    An efficient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5-100 μM) for different time duration (4, 8, 12 and 16 d), proved a significant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation. The highest regeneration rate (85%), average number of shoots/explant (8.7 ± 0.22) and maximum shoot length (3.9 ± 0.33 cm) were obtained from the nodal explants exposed to 50 μM TDZ for 8 d. The nodal explants excised from the proliferated cultures of TDZ (50 μM) for 8 d were used as explants and showed an enhancement rate after next three round of in vitro propagation. Best results for rooting was obtained by ex vitro treatment of shoots with 200 μM indole-3-butyric acid (IBA) for 20 min. as it produced an average of 5.7 ± 0.41 roots per microshoot with 4.4 ± 0.39 cm root length in 84% shoots. Different planting substrates was tested for maximum survival of hardening off micropropagated plantlets and soilrite proved most effective than others as 97.1 ± 7.21 plantlets survived. All micropropagated plants grew well in natural conditions and showed similar morphology to the mother plant.

  12. Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micropropagation of Capsicum annuum.

    Science.gov (United States)

    Asgari-Targhi, Ghasem; Iranbakhsh, Alireza; Ardebili, Zahra Oraghi

    2018-06-01

    Concerning environmental issues of metal based-nanomaterials and increasing demand for nano-based products; various strategies have been employed to find eco-friendly natural nano-compounds, among which nano-polymer chitosan is mostly considered. Herein, the various aspects of the way in which bulk or nano-chitosan may modify growth, morphogenesis, micropropagation, and physiology of Capsicum annuum L. were considered. Culture medium was manipulated with different concentrations of bulk chitosan or synthesized chitosan/tripolyphosphate (TPP) nano-particle. The supplementations of culture media led to changes in morphology (especially, the root architecture) and differentiation. Toxic doses of bulk (100 mgL -1 ) or nano-chitosan (5, 10, and 20 mgL -1 ) dramatically provoked cessation of plant growth and development. Plant growth and biomass accumulations were increased along with the suitable levels of bulk or nano-chitosan. Peroxidase and catalase activities in a dose and organ-dependent manners were significantly modified by the supplements. Phenylalanine ammonia lyase was induced by the mentioned supplements. Also, the contents of soluble phenols, proline, and alkaloid were found to be significantly increased by the elicitors, over the control. The nano-chitosan of 1 mgL -1 was found to be the most effective elicitor to trigger organogenesis via micropropagation. The huge differences between triggering and toxic concentrations of the supplements would be due to the physicochemical modifications of nano-polymeric. Furthermore, the results highlight the potential benefits (hormone-like activity) and phytotoxic impacts of nano-chitosan/TPP for in vitro manipulations. This is the first report on both the favorable and adverse effects of nano-chitosan/TPP, representing requirements for further investigation on such formulations for future applications. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

  13. SemEHR: A general-purpose semantic search system to surface semantic data from clinical notes for tailored care, trial recruitment, and clinical research.

    Science.gov (United States)

    Wu, Honghan; Toti, Giulia; Morley, Katherine I; Ibrahim, Zina M; Folarin, Amos; Jackson, Richard; Kartoglu, Ismail; Agrawal, Asha; Stringer, Clive; Gale, Darren; Gorrell, Genevieve; Roberts, Angus; Broadbent, Matthew; Stewart, Robert; Dobson, Richard J B

    2018-05-01

    Unlocking the data contained within both structured and unstructured components of electronic health records (EHRs) has the potential to provide a step change in data available for secondary research use, generation of actionable medical insights, hospital management, and trial recruitment. To achieve this, we implemented SemEHR, an open source semantic search and analytics tool for EHRs. SemEHR implements a generic information extraction (IE) and retrieval infrastructure by identifying contextualized mentions of a wide range of biomedical concepts within EHRs. Natural language processing annotations are further assembled at the patient level and extended with EHR-specific knowledge to generate a timeline for each patient. The semantic data are serviced via ontology-based search and analytics interfaces. SemEHR has been deployed at a number of UK hospitals, including the Clinical Record Interactive Search, an anonymized replica of the EHR of the UK South London and Maudsley National Health Service Foundation Trust, one of Europe's largest providers of mental health services. In 2 Clinical Record Interactive Search-based studies, SemEHR achieved 93% (hepatitis C) and 99% (HIV) F-measure results in identifying true positive patients. At King's College Hospital in London, as part of the CogStack program (github.com/cogstack), SemEHR is being used to recruit patients into the UK Department of Health 100 000 Genomes Project (genomicsengland.co.uk). The validation study suggests that the tool can validate previously recruited cases and is very fast at searching phenotypes; time for recruitment criteria checking was reduced from days to minutes. Validated on open intensive care EHR data, Medical Information Mart for Intensive Care III, the vital signs extracted by SemEHR can achieve around 97% accuracy. Results from the multiple case studies demonstrate SemEHR's efficiency: weeks or months of work can be done within hours or minutes in some cases. SemEHR provides a more

  14. Notes on Laser Acceleration

    International Nuclear Information System (INIS)

    Tajima, T.

    2008-01-01

    This note intends to motivate our effort toward the advent of new methods of particle acceleration, utilizing the fast rising laser technology. By illustrating the underlying principles in an intuitive manner and thus less jargon-clad fashion, we seek a direction in which we shall be able to properly control and harness the promise of laser acceleration. First we review the idea behind the laser wakefield. We then go on to examine ion acceleration by laser. We examine the sheath acceleration in particular and look for the future direction that allows orderly acceleration of ions in high energies

  15. MISCELLANEOUS BOTANICAL NOTES 2

    Directory of Open Access Journals (Sweden)

    A.J.G.H KOSTERMANS

    2014-01-01

    Full Text Available 1.   Durio  cupreus Ridley is considered to  represent a  distinct  species.2.   Durio wyatt-smithii Kosterm. is reported from Borneo.3.   Machilus nervosa Merr. represents Meliosma bontoeensis Merr.4.   Beilschmiedia brassii Allen represents Vavaea brassii (Allen Kosterm.5.   The author of the generic name Heritiera is Aiton.6.   Heritiera macrophylla (non Wall. Merr. is conspecific with H. ungus-tata Pierre.7.   Some specimens from N. Celebes, attributed formerly to H. sylvatica Merr., belong to H. arafurensis Kosterm.8.   Additional note on Heritiera littoralis Ait. and H. macrophylla Wall, ex Kurz.9.   Heritiera   montana   Kosterm.,   nov.   spec,   from   New   Guinea   and H. khidii Kosterm., nov. spec, from Northern Siam.10.   Additional note on Heritiera, novoguineensis Kosterm. and H. pereo-riacea Kosterm. and an undescribed species.11.   Heritiera acuminata Wall, ex Kurz represents a distinct species.12.   Heritiera  solomonensis  Kosterm.,  nov.  spec,  from the  Solomon  Isl.13.   A note on Firmiana bracteata A. DC.14.   Firmiana fulgens (Wall, ex King  Corner is based on a mixtum com-positum and has been the source of constant confusion. For the element, which occurs in Malaysia a new name is coined: F. malayana Kosterm. It does not occur in Tenasserim.15.   A revised bibliography of Firmiana colorata R. Br., F. pallens Stearn and F. malayana Kosterm. is presented.16.   Additional note on Firmiana hainanensis Kosterm.17.   Firmiana kerrii (Craib Kosterm., comb, nov., based on Sterculia kerrii Craib.18.   Additional specimens of Firmiana papuana Mildbr.19.   Cryptocarya hintonii Allen is referred to Primus as Primus hintonii (Allen  Kosterm.20.   Beilschmiedia wallichiana (G. Don   Kosterm., based on Sideroxylon wallichianum, G. Don, is described. Formerly it was relegated to Litsea by Kurz.21.   New species in Lauraceae: Beilschmiedia aborensis Kosterm., B

  16. Notes on functional analysis

    CERN Document Server

    Bhatia, Rajendra

    2009-01-01

    These notes are a record of a one semester course on Functional Analysis given by the author to second year Master of Statistics students at the Indian Statistical Institute, New Delhi. Students taking this course have a strong background in real analysis, linear algebra, measure theory and probability, and the course proceeds rapidly from the definition of a normed linear space to the spectral theorem for bounded selfadjoint operators in a Hilbert space. The book is organised as twenty six lectures, each corresponding to a ninety minute class session. This may be helpful to teachers planning a course on this topic. Well prepared students can read it on their own.

  17. Essential oil obtained from micropropagated lavender, its effect on HSF cells and application in cosmetic emulsion as a natural protective substance.

    Science.gov (United States)

    Andrys, D; Adaszyńska-Skwirzyńska, M; Kulpa, D

    2018-04-01

    The aim of the study was to determine the influence of the essential oils isolated from the field - grown and micropropagated in vitro narrow - leaved lavender of the 'Munstead' cultivar, on human skin cells, and their capability to synthesise procollagen. The amount of procollagen type I produced by fibroblast cells was determined using ELISA kit. Essential oil isolated from micropropagated lavender was further used as a protective ingredient against the development of microorganisms in O/W cosmetic emulsion. The presented results demonstrate that the use of 0.01, 0.001 and 0.0001% essential oils isolated from in vitro plants stimulate HSF cells to the production of procollagen. It was further performed that the tested essential oil used in the concentration of 0.1% in a cosmetic emulsion is characterised by preservative effect for cosmetic preparations for the period of 3 months.

  18. An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop.

    Science.gov (United States)

    Choudhary, Sumitra Kumari; Patel, Ashok Kumar; Harish; Shekhawat, Smita; Shekhawat, Narpat S

    2017-07-01

    Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L -1 BAP, 0.1 mg L -1 IAA and additives (50 mg L -1 ascorbic acid, 25 mg L -1 each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L -1 IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female M. dioica , a popular summer vegetable crop.

  19. RESEARCH NOTE EFFECTIVENESS OF PARAQUAT ON THE ...

    African Journals Online (AJOL)

    The remaining area received no chemical treatment and the grass was left to die at first frost (May l0). Thirty Africander cross Sussex steers, approxi- mately l0 rnonths of age, were randomly allocated to the 2 treatments. From July 20 until September 28. (70 days), both treatment areas were grazed by the respective groups.

  20. Research Notes - Openness and Evolvability - Standards Assessment

    Science.gov (United States)

    2016-08-01

    an unfair advantage. The company not only has the opportunity to be faster to market , but can also impose a level of control on its competitors...The independence of different vendors’ implementations must be carefully assessed to ensure a monopolistic or oligopolistic condition does not exist...political affiliation they may have with other implementation vendors. However, this is unlikely to be practical in markets where the customer is not a

  1. Research Notes -- Openness and Evolvability -- Legal Assessment

    Science.gov (United States)

    2016-08-01

    against obsolescence in place?............................... 6 2.1.13 Is compliance with shared infrastructures legally enforceable? ....... 6 2.1.14 Is...bundling acceptance criteria and milestone delivery schedules . This may hide the possibility of unpublished coupling being incorporated between bundled...2.1.12 Is legal protection against obsolescence in place? If the standards used by support tools are not open, determine whether contract or similar

  2. RESEARCH NOTE THE LIKELY EFFICMNCY OF PRENATAL ...

    African Journals Online (AJOL)

    A solution to this problem is to estimate the energy content of the animal body at birth e.g.by the use of a regression equation relating birth mass and body ... adiabetic bomb calorimeter, and 38 were hatched in an incubator. Of the latter 30 were infertile, 6 died during incubation, while only 2 eggs hatched. In spite of this.

  3. Elderly homicide in Chicago: a research note.

    Science.gov (United States)

    Fazel, Seena; Bond, Mieko; Gulati, Gautam; O'Donnell, Ian

    2007-01-01

    There is a body of knowledge about elderly crime victims, but we know little about the characteristics of elderly perpetrators, especially of homicide. The few studies that have been published are based on small samples and are somewhat dated. In an examination of homicides committed in Chicago over a 31-year period, we compared cases involving perpetrators aged over 60 years (n = 443) with those involving younger perpetrators (n = 24,066). There were a number of significant differences. Elderly killers were more likely to be White and to commit suicide afterwards. Their victims were more likely to be spouses, females, and aged over 60 years. Inter-racial homicide was more common for younger offenders. The low number of homicides involving the elderly is explained by reference to routine activity theory. Copyright 2007 John Wiley & Sons, Ltd.

  4. Research Notes ~ Electronic Tutorials: Indonesian Experience

    Directory of Open Access Journals (Sweden)

    Tian Belawati

    2002-04-01

    Full Text Available As in other developing nations, important concerns surrounding education in Indonesia involve two issues: quantity versus quality. Quality concerns have now been somewhat addressed by the establishment of the Indonesian Open Learning University (Universitas Terbuka in 1984. The concern for quality, however, has not yet been completely resolved. Learning support, believed to be key for achieving good quality distance education, has been limited. This paper presents the results of two pilot projects that examined tutorials provided via Internet and Fax-Internet technologies. It is a report that also shows that the Universitas Terbuka is faced with both visible and invisible challenges. Visible challenges include limitations in the availability of technology infrastructure and issues of inadequate access, while invisible challenges include the readiness of Indonesian people to adopt and take advantage of new technology for educational purposes. Despite the results of the pilot project, it is suggested that Universitas Terbuka should continue to utilize Internet and Fax-Internet as two of its communication channels with students.

  5. Pets or People: Another Research Note.

    Science.gov (United States)

    Goldmeier, John

    1986-01-01

    Compared four samples of elderly women: living alone, with other persons, and with and without a pet. Pets only made a difference for those living alone. At best, pets only attenuate the sense of loneliness. In intervention with the elderly, the provision of human supports should remain a priority. (Author/BL)

  6. Research Note Development and characterization of microsatellite ...

    Indian Academy of Sciences (India)

    Srirama R

    2School of Biosciences and Technology, VIT University, Vellore 632014, India. 7. 8 ... microsatellite markers developed can be used for studying the population ... characterized by diverse growth forms, including shrubs, trees, and annual or ...

  7. Research Note: Predicting Leucaena leucocephala biomass from ...

    African Journals Online (AJOL)

    The amount of leaf and stem material removed by browsers can be precisely (r2 >0.90) and accurately (within 11% of the mean) predicted from DPB, thus providing a simple means of estimating browse offtake from leucaena trees. Keywords: Browse, firewood, regression, tree height, twig diameter. African Journal of Range ...

  8. RESEARCH NOTE Rapid isolation and characterization of ...

    Indian Academy of Sciences (India)

    fraser c

    2017-10-25

    Oct 25, 2017 ... untrue for non-model organisms. Here, we use a fast, ... genetically depauperate (based on mitochondrial gene sequencing; Faria et al. 2011), there is no ... modified workflow described by Griffiths et al. (2016). Our method ...

  9. Disengaging from Workplace Relationships. A Research Note

    Science.gov (United States)

    Sias, Patricia M.; Perry, Tara

    2004-01-01

    A randomly-selected sample of 306 adults employed full-time rated how likely individuals would be to use various communication strategies (cost escalation, depersonalization, and state-of-the-relationship talk) to disengage from a workplace relationship (with the target of the deteriorations being a supervisor, a peer coworker, or a subordinate…

  10. Research Note Consumer Addressability and Customized Pricing

    OpenAIRE

    Yuxin Chen; Ganesh Iyer

    2002-01-01

    The increasing availability of customer information is giving many firms the ability to reach and customize price and other marketing efforts to the tastes of the individual consumer. This ability is labeled as consumer addressability. Consumer addressability through sophisticated databases is particularly important for direct-marketing firms, catalog retailers such as L.L Bean and Land's End, credit card-issuing banks, and firms in the long-distance telephone market. We examine the strategic...

  11. Research Note Development and characterization of microsatellite ...

    Indian Academy of Sciences (India)

    Srirama R

    provide important information in developing sustainable harvesting practices. .... United States Agency for International Development (USAID, Award number: ... Rozen S., Skaletsky H. 2000 Primer3 on the WWW for general users and for ...

  12. RESEARCH NOTE Parentage identification of Odontobutis ...

    Indian Academy of Sciences (India)

    44738

    Microsatellite-based parentage identification technology has been widely used .... (201505059), Jiangsu Province Six Talent Peaks of High Level Talents Project ... Kalinowski S. T., Taper M. L., Marshall T. C. 2007 Revising how the computer.

  13. Research notes : monitoring water quality along highways.

    Science.gov (United States)

    2006-12-01

    Runoff from highways typically picks up a variety of pollutants from the roadway. These pollutants include sediment, trash, residue from petroleum products, and heavy metals. Depending on the highway and its geographic setting, highway runoff can eva...

  14. Note e Recensioni

    Directory of Open Access Journals (Sweden)

    a cura di Mariagrazia Portera

    2013-12-01

    Full Text Available Volumi Winfried Menninghaus, La promessa della bellezza, [Fabrizio Desideri, p. 272] • David Rothenberg, Survival of the Beautiful. Art, Science and Evolution [Danae Crocchiola, p. 274] • Lev Manovich, Software Takes Command [Angela Maiello, p. 277]. Note Method in Aesthetics: Philosophy, Evolution and the Cognitive Sciences [Aaron Meskin, Matthew Kieran, Gregory Currie, p. 280] • L’Abitare possibile. Estetica, Architettura e New Media, Ravello, Auditorium Oscar Niemeyer, 28-30 maggio 2013 [Sara Matetich, p. 282] • Copenhagen Summer School in Phenomenology and Philosophy of Mind, University of Copenhagen, 12-16 Agosto 2013 [Raoul Frauenfelder, p. 289] • Ciò che è vivo e ciò che è morto nell’estetica di Archibald Ali- son. Nota a margine del convegno: Neoestetica ed emozione. Archibald Alison e l’estetica con- temporanea, Palermo, 4-5 ottobre 2013 [Giuseppe Pucci, p. 294

  15. 36 CFR 1254.12 - Will NARA log or inspect my computer, other equipment, and notes?

    Science.gov (United States)

    2010-07-01

    ... personal notes into the research room. In research rooms that permit taking in your notes, a NARA or... computer, other equipment, and notes? 1254.12 Section 1254.12 Parks, Forests, and Public Property NATIONAL... MATERIALS General Information § 1254.12 Will NARA log or inspect my computer, other equipment, and notes? (a...

  16. Potential Applications of the National Institute of Mental Health's Research Domain Criteria (RDoC) to Clinical Psychiatric Practice: How RDoC Might Be Used in Assessment, Diagnostic Processes, Case Formulation, Treatment Planning, and Clinical Notes.

    Science.gov (United States)

    Yager, Joel; Feinstein, Robert E

    2017-04-01

    Offering a new framework for understanding and studying basic dimensions of normal and abnormal human functioning and mental disorders, the National Institute of Mental Health (NIMH) has initiated the Research Domain Criteria (RDoC) project in which a series of higher order domains, representing major systems of emotion, cognition, motivation, and social behavior, and their constituent operationally defined constructs serve as organizing templates for further research and inquiry, eg, to discover validated biomarkers and endophenotypes. Cutting across traditional DSM diagnoses, the domains are defined as Negative Valence Systems, Positive Valence Systems, Cognitive Systems, Systems for Social Processes, and Arousal/Regulatory Systems. To inform educators, trainees, and practitioners about RDoC, alert them to potential practical applications, and encourage their broad exploration in clinical settings, this article reviews the RDoC domains and their subsystem constructs with regard to potential current clinical considerations and applications. We describe ways in which the RDoC domains and constructs offer transdiagnostic frameworks for complementing traditional practice; suggest clinical questions to help elucidate salient information; and, translating RDoC domains and constructs headings into clinically friendly language, offer a template for the psychiatric review of systems that can serve in clinical notes. © Copyright 2017 Physicians Postgraduate Press, Inc.

  17. NOTES: issues and technical details with introduction of NOTES into a small general surgery residency program.

    Science.gov (United States)

    Kavic, Michael S; Mirza, Brian; Horne, Walter; Moskowitz, Jesse B

    2008-01-01

    laparoscopic observation of NOTES maneuvers. Necropsy was performed to determine specific details of surgical intervention. NOTES intervention is feasible in an animal model. Insight into the potential of NOTES was obtained in this investigation. NOTES investigation in a controlled, laboratory setting using an animal model proved to have value for our program. A steep learning curve was encountered despite the availability of an investigator familiar with elementary NOTES procedures. The authors strongly suggest investigators adopt the ASGE/SAGES working group recommendations for a multidisciplinary team possessing advanced therapeutic endoscopic and advanced laparoscopic skills to study NOTES before human investigation. Animal laboratory facilities to perform research and training should be available to the multidisciplinary team for exploration of NOTES techniques and procedures. Institutional Review Board (IRB) approval must be obtained before introduction of NOTES procedures in human patients.

  18. European Science Notes. Volume 41, Number 9.

    Science.gov (United States)

    1987-09-01

    management system and of the following: Secretaria de la Revista the inference engine. de Psicologia Social , Facultad de Psico- Application of Knowledge-Based...Notes NATO Advanced Research Workshop on Social and Environmental Psychology .................... William D. Crano 519 Applied Artificial Intelligence...An International Journal ................................. William D. Crano 520 New Spanish Journal of Social Psychology ................. William D

  19. Note on Plagiarism

    Indian Academy of Sciences (India)

    These are consequences of the increasing pressures to publish for career advancement, competition for research support and desire for recognition, influence and fame. In the. USA an Office of Research Integrity has been created, dealing largely with the biological and biomedical fields. In recent years innumerable ...

  20. Concept Note: Environmental Economics

    International Development Research Centre (IDRC) Digital Library (Canada)

    TEST

    IDRC has a long-standing reputation for supporting good agricultural and natural .... Effective ways to scale up sustainable land management options need to be ..... graduate students conducting their research within specific projects involving.

  1. Editor’s Note

    Directory of Open Access Journals (Sweden)

    Manju Khari

    2018-06-01

    Full Text Available The International Journal of Interactive Multimedia and Artificial Intelligence (IJIMAI provides an interdisciplinary forum in which scientists and professionals share their research results and report new advances on tools that use AI with interactive multimedia techniques.

  2. Notes in Colombian Herpetology, II Notes in Colombian Herpetology, II

    Directory of Open Access Journals (Sweden)

    Dunn Emmett Reid

    1944-03-01

    Full Text Available The Lizard Genus Echinosaura (Teiidae in Colombia / Notes on the habits of the Tadpole-Carrying Frog Hyloxalus granuliventris / A New Marsupian Frog (Gastrotheca from Colombia The Lizard Genus Echinosaura (Teiidae in Colombia / Notes on the habits of the Tadpole-Carrying Frog Hyloxalus granuliventris / A New Marsupian Frog (Gastrotheca from Colombia.

  3. Note to Self

    DEFF Research Database (Denmark)

    Hansen, Lone Koefoed; Dalsgaard, Peter

    2015-01-01

    The term “natural” is employed to describe a wide range of novel interactive products and systems, ranging from gesture-based interaction to brain-computer interfaces and in marketing as well as in research. However, this terminology is problematic. It establishes an untenable dichotomy between...... forms of interaction that are natural and those that are not; it draws upon the positive connotations of the term and conflates the language of research with marketing lingo, often without a clear explanation of why novel interfaces can be considered natural; and it obscures the examination...... of interfaces that we have hitherto labelled “natural”....

  4. Notes on Contributors

    African Journals Online (AJOL)

    He is a Fellow of the Cambridge Commonwealth Society and member of the International Society for Third Sector Research (ISTR). Daniel Eseme Gberevbie, Associate Professor, Department of Political Science and International Relations, Covenant University, Ota Ogun State, Nigeria. AJOL African Journals Online.

  5. Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process.

    Science.gov (United States)

    Duarte-Aké, Fátima; Castillo-Castro, Eduardo; Pool, Felipe Barredo; Espadas, Francisco; Santamaría, Jorge M; Robert, Manuel L; De-la-Peña, Clelia

    2016-12-01

    Global DNA methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in Agave angustifolia Haw. While the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. During the micropropagation of Agave angustifolia Haw., we found three different phenotypes, green (G), variegated (V) and albino (A). To understand the physiological and epigenetic differences among the somaclones, we analyzed several morphophysiological parameters and changes in the DNA methylation patterns in the three phenotypes during their in vitro development. We found that under in vitro conditions, the V plantlets maintained their CAM photosynthetic capacity, while the A variant showed no pigments and lost its CAM photosynthetic ability. Epigenetic analysis revealed that global DNA methylation increased in the G phenotype during the first two subcultures. However, after that time, DNA methylation levels declined. This hypomethylation correlated with the appearance of V shoots in the G plantlets. A similar correlation occurred in the V phenotype, where an increase of 2 % in the global DNA methylation levels was correlated with the generation of A shoots in the V plantlets. This suggests that an "epigenetic stress memory" during in vitro conditions causes a chromatin shift that favors the generation of variegated and albino shoots.

  6. Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants.

    Science.gov (United States)

    Shinde, Smita; Sebastian, Joseph Kadanthottu; Jain, Jyothi Ramesh; Hanamanthagouda, Manohar Shirugumbi; Murthy, Hosakatte Niranjana

    2016-10-01

    A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 µM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was sub-cultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 µM) along with 7.5 µM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 µM BAP + 7.5 µM 2-iP. Quarter strength MS medium supplemented with 10 µM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.

  7. A xyloglucan from seeds of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples.

    Science.gov (United States)

    Lima-Nishimura, N; Quoirin, M; Naddaf, Y G; Wilhelm, H M; Ribas, L L F; Sierakowski, M-R

    2003-01-01

    Xyloglucan was extracted from seeds of Hymenaea courbaril and mixed with agar to prepare a solid culture medium used for micropropagation of the Marubakaido apple rootstock (Malus prunifolia Borkh) and cv. Jonagored (Malus domestica). The performance on gels created from a blend of 0.4%agar and 0.2% xyloglucan (w/v) was compared with that on media gelled with a standard concentration 0.6% (w/v) of agar. The growth of shoots and the multiplication rate were higher on the modified culture medium than on the agar-gelled medium. The occurrence of hyperhydric shoots was lower on the modified medium. In the absence of auxin, shoot rooting reached 70% (Marubakaido) and 66% (Jonagored) on the agar-xyloglucan medium and 6.7% and 10.4%, respectively, on the agar medium. When 0.25 microM indole-3-butyric acid (IBA) was added to both media, the modified medium gave better results in terms of rooting percentage and quality of roots than the agar-gelled medium.

  8. Sphagnum restoration on degraded blanket and raised bogs in the UK using micropropagated source material: a review of progress

    Directory of Open Access Journals (Sweden)

    S.J.M Caporn

    2018-05-01

    Full Text Available There is a growing demand for a supply of Sphagnum to re-introduce to degraded peatlands. However, available supplies of Sphagnum of the desired species are often limited. We describe the propagation of Sphagnum from vegetative material in sterile tissue culture and the introduction of juvenile mosses into the field. Sphagnum produced in the laboratory in three different forms (beads, gel and plugs was introduced to different peatland surfaces on upland degraded blanket bog and lowland cut-over peatland in northern England. On degraded blanket bog, the establishment of mixed-species Sphagnum plugs was typically 99 % while the survival of beads was much lower, ranging from little above zero on bare eroding peat to a maximum of 12 % on stabilised peat surfaces. On lowland cut-over peatland, all trials took place on peat with an expanding cover of Eriophorum angustifolium and tested Sphagnum gel as well as beads and plugs. This work showed that survival and establishment of plugs was high (99 % and greater than for beads. Sphagnum gel reached a cover of 95 % in two years. The vegetative micropropagation of Sphagnum offers an effective source of Sphagnum for re-introduction to degraded peatlands.

  9. A note on axial symmetries

    International Nuclear Information System (INIS)

    Beetle, Christopher; Wilder, Shawn

    2015-01-01

    This note describes how to characterize and normalize an axial Killing field on a general Riemannian geometry or four-dimensional Lorentzian geometry. No global assumptions are necessary, such as that the orbits of the Killing field all have period 2π. Rather, any Killing field that vanishes at at least one point necessarily has the expected global properties. (note)

  10. The Anatomy of a Note.

    Science.gov (United States)

    Moore, Herb

    1986-01-01

    Suggests that students can learn the physics of a musical note by learning how to synthesize sounds on a computer. Discusses ADSR (attack, decay, sustain, and release of a note) and includes a program (with listing) which students can use to examine ADSR on a Commodore 64 microcomputer. (JN)

  11. Parallel Note-Taking: A Strategy for Effective Use of Webnotes

    Science.gov (United States)

    Pardini, Eleanor A.; Domizi, Denise P.; Forbes, Daniel A.; Pettis, Gretchen V.

    2005-01-01

    Many instructors supply online lecture notes but little attention has been given to how students can make the best use of this resource. Based on observations of student difficulties with these notes, a strategy called parallel note-taking was developed for using online notes. The strategy is a hybrid of research-proven strategies for effective…

  12. Deformation Theory ( Lecture Notes )

    Czech Academy of Sciences Publication Activity Database

    Doubek, M.; Markl, Martin; Zima, P.

    2007-01-01

    Roč. 43, č. 5 (2007), s. 333-371 ISSN 0044-8753. [Winter School Geometry and Physics/27./. Srní, 13.01.2007-20.01.2007] R&D Projects: GA ČR GA201/05/2117 Institutional research plan: CEZ:AV0Z10190503 Keywords : deformation * Mauerer-Cartan equation * strongly homotopy Lie algebra Subject RIV: BA - General Mathematics

  13. Notes on Contributors

    Directory of Open Access Journals (Sweden)

    Notes on Contributors

    2013-06-01

    Full Text Available Aileen Ackland is a teacher educator at the School of Education, University of Aberdeen, Scotland. From 2006 to 2012 she was the Curriculum and Research Leader for the Scottish Consortium which developed and delivered the first professional qualification for Scottish Adult Literacies practitioners. Prior to joining the university, she worked in Adult and Community Education, mainly in the voluntary sector.

  14. Notes on continuum mechanics

    CERN Document Server

    Chaves, Eduardo W V

    2013-01-01

    This publication is aimed at students, teachers, and researchers of Continuum Mechanics and focused extensively on stating and developing Initial Boundary Value equations used to solve physical problems. With respect to notation, the tensorial, indicial and Voigt notations have been used indiscriminately.   The book is divided into twelve chapters with the following topics: Tensors, Continuum Kinematics, Stress, The Objectivity of Tensors, The Fundamental Equations of Continuum Mechanics, An Introduction to Constitutive Equations, Linear Elasticity, Hyperelasticity, Plasticity (small and large deformations), Thermoelasticity (small and large deformations), Damage Mechanics (small and large deformations), and An Introduction to Fluids. Moreover, the text is supplemented with over 280 figures, over 100 solved problems, and 130 references.

  15. Editors note, and Dedication

    Directory of Open Access Journals (Sweden)

    Laura Mugnai

    2011-12-01

    Full Text Available This Supplement to volume 50 of Phytopathologia Mediterranea contains original, peer reviewed research papers, prepared from presentations at the 7th International Workshop on Grapevine Trunk Diseases (IWGTD. This Workshop was held in Santa Cruz, Chile, 17–21 January 2010, and was organized by the International Council on Grapevine Trunk Diseases (ICGTD.  Publication of this Supplement has been financially assisted by the International Society for Plant Pathology (ISPP.This Supplementary Issue of Phytopathologia Mediterranea is dedicated to the memory of Dr Luigi Chiarappa, the founder and inspiration of the ICGTD.  

  16. Denmark country note

    DEFF Research Database (Denmark)

    Bloksgaard, Lotte; Rostgaard, Tine

    2014-01-01

    The LP&R network produces an annual review of leave policies and related research, starting in 2004 (for earlier reviews, go to Archive 2005-2013). The review covers Maternity, Paternity and Parental leaves; leave to care for sick children and other employment-related measures to support working......, Israel. Altogether, it covers 35 countries. In addition to Israel, these are: Austria, Australia, Belgium, Brazil, Canada, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Japan, Lithuania, Luxembourg, Netherlands, New Zealand, Norway, Poland...

  17. Notes on the Spatial Turn

    Directory of Open Access Journals (Sweden)

    Stipe Grgas

    2012-06-01

    Full Text Available On the basis of ever-mounting evidence, amongst which is the “zone” problematic of the Zadar conference that occassioned these notes, it can be concluded that the spatial turn has insinuated itself as an all-pervading heuristic tool throughout the humanities and the social sciences. The extent to which space and spatiality have usurped the central stage in the various branches of reasearch can be gauged by admonishments that what we are witnessing is a new fundamentalism that has simply inverted the terms of the dualism of time and space (May and Thrift 2001: “Introduction”. According to Michael Dear the sway of space is manifested in multifold ways: in the ubiquity of spatial analysis in social theories and practices; in the explosion of publications devoted to the exploration of the interface of the social and the spatial; in the reintegration of human geography into various domains of knowledge; in the focus given to difference and the consequent diversification of theoretical and empirical practices; in a theoretically informed exploration of the relation between geographical knowledge and social action; and, finally, in the unprecedented proliferation of research agendas and publications pertaining to these isuuses (Dear 2001: 24. Two recent collections of papers are indicative of the ubiquity of spatial issues in scholarly work.

  18. Editor’s note

    Directory of Open Access Journals (Sweden)

    Vladimir D. Tonchev

    2016-09-01

    Full Text Available This Special Issue, entitled Algebraic Combinatorics and Applications, of the Journal of Algebra,Combinatorics, Discrete Structures, and Applications, contains selected papers submitted by conferenceparticipants at the "Algebraic Combinatorics and Applications: The First Annual Kliakhandler Conference",Houghton, Michigan, USA, August 26 - 30, 2015, as well as two additional papers submitted inresponse to our call for papers.The conference took place on the campus of Michigan Technological University, and was attendedby 43 researchers and graduate and postdoctoral students from USA, Canada, Croatia, Japan, SouthAfrica, and Turkey. Funding for the conference was provided by a generous gift of Igor Kliakhandler,and a grant from the National Science Foundation. The conference brought together researchers andstudents interested in combinatorics and its applications, to learn about the latest developments, andexplore different visions for future work and collaborations. Over thirty talks were presented on varioustopics from combinatorial designs, graphs, finite geometry, and their applications to error-correctingcodes, network coding, information security, quantum computing, DNA codes, mobile communications,and tournament scheduling.The current Special Issue contains papers on covering arrays and their applications, group divisibledesigns, automorphism groups of combinatorial designs, covering number of permutation groups, tournaments,large sets of geometric designs, partitions, quasi-symmetric functions, resolvable Steiner systems,and weak isometries of Hamming spaces.

  19. Suicide Notes in Mexico: What Do They Tell Us?

    Science.gov (United States)

    Chavez-Hernandez, Ana-Maria; Paramo, Daniel; Leenaars, Antoon A.; Leenaars, Lindsey

    2006-01-01

    According to international and Mexican official statistics, there is a dramatic rise in suicide in Mexico; however, research in this area is severely limited. This is the first study of suicide notes from Mexico in the international literature. From a population of 747 registered suicides, a sample of 106 note-writers and 106 nonnote writers was…

  20. Notes on Hadza cosmology

    DEFF Research Database (Denmark)

    Skaanes, Thea

    2015-01-01

    Abstract: This article concerns Hadza cosmology examined through objects, rituals and the Hadza concept of epeme. A brief background to the Hadza and the eldwork that informs this study is followed by a close analysis of three key objects that are central to the argument presented. The objects...... are intimately linked to women and to aspects of the social and cosmological identity of the individual makers. one object is a materi- alisation of the woman’s name and it leads to an examination by interview of naming practices more generally. Naming a child gives it a spirit and places the child in a strong...... of ethnographic research indicating the potential and need for further examination of the power and role of objects in Hadza society. Keywords: Hadza, epeme, ritual, cosmology, power objects...

  1. Digital Note-Taking: Discussion of Evidence and Best Practices.

    Science.gov (United States)

    Grahame, Jason A

    2016-03-01

    Balancing active course engagement and comprehension with producing quality lecture notes is challenging. Although evidence suggests that handwritten note-taking may improve comprehension and learning outcomes, many students still self-report a preference for digital note-taking and a belief that it is beneficial. Future research is warranted to determine the effects on performance of digitally writing notes. Independent of the methods or software chosen, best practices should be provided to students with information to help them consciously make an educated decision based on the evidence and their personal preference. Optimal note-taking requires self-discipline, focused attention, sufficient working memory, thoughtful rewording, and decreased distractions. Familiarity with the tools and mediums they choose will help students maximize working memory, produce better notes, and aid in their retention of material presented.

  2. Note-taking in the employment interview: effects on recall and judgments.

    Science.gov (United States)

    Middendorf, Catherine Houdek; Macan, Therese Hoff

    2002-04-01

    Although note-taking in the employment interview is highly recommended, little research has examined its effects. This study investigated the effects of note-taking styles, review of the notes, and content of the notes on participants' cued recall of information and decisions made from videotaped employment interviews. Note-taking increased recall accuracy but not judgment accuracy. Being able to review notes resulted in increased judgment accuracy for those taking conventional-style notes. The content of the notes also had important implications for conventional note-takers, suggesting some benefits of recording notes using the key-points style. The findings suggest that the act of note-taking may be more important for memory and legal reasons than for improving the decisions made by interviewers.

  3. Medical students' note-taking in a medical biochemistry course: an initial exploration.

    Science.gov (United States)

    Morrison, Elizabeth H; McLaughlin, Calvin; Rucker, Lloyd

    2002-04-01

    Beginning medical students spend numerous hours every week attending basic science lectures and taking notes. Medical faculty often wonder whether they should give students pre-printed instructors' notes before lectures. Proponents of this strategy argue that provided notes enhance learning by facilitating the accurate transmission of information, while opponents counter that provided notes inhibit students' cognitive processing or even discourage students from attending lectures. Little if any research has directly addressed medical students' note-taking or the value of providing instructors' notes. The educational literature does suggest that taking lecture notes enhances university students' learning. University students perform best on post-lecture testing if they review a combination of provided notes and their own personal notes, particularly if the provided notes follow a 'skeletal' format that encourages active note-taking.

  4. Editorial Note vol. 47

    Directory of Open Access Journals (Sweden)

    Oswaldo Lorenzo

    2017-12-01

    Full Text Available Issue  47  of  Publicaciones brings  excellent  news  for  this  research  journal:  from October 2017 it is included in the prestigious Scopus database. This achievement has been made possible by two factors: on the one hand, all the work done by previous editors-in- chief of Publicaciones, the Editorial Board, the Advisory Board, the peer reviewers, the Administration and Services staff of the Melilla university campus, the various interns the journal has had, and our sponsors, the Department of Education, Youth and Sport of the Autonomous City of Melilla and the Office of the Vice-Rector for Research and Knowledge Transfer of the University of Granada. On the other hand, the new strategy of internationali- sation and visibility on social media that the journal has been following since July 2016 has also made an important contribution. During the past year, Publicaciones has been presented in higher education institutions in China (Shanghai International Studies University and Peking University and Mexico (Autonomous University of Yucatán and Centre for Teaching and Research of the Ministry of Education of the Yucatán State Government, and it will continue on this path of interna- tionalisation over the next few years. It has also established collaboration agreements with a number of institutions and international education networks to publish special issues. As for its presence on social media, work has been done on search engine optimisation (SEO and networks, web traffic monitoring, updating the Google Scholar Citations profile and creating a new Facebook profile. All this is gradually building a process of national and international growth and expan- sion for the journal, which will continue seeking to be included in new document collections and databases and to increase its quality factors and indicators. Turning now to the content of this issue, number 47, it begins with a study on the use of information and communication

  5. Editor’s note

    Directory of Open Access Journals (Sweden)

    André Leroy

    2017-01-01

    Full Text Available This special issue contains papers written by participants to the conference "Noncommutative rings and their Applications" that was held in Lens (France at the Science faculty of the Universit\\'e d'Artois. The meeting gave the experts from different domains the opportunity to exchange their views, share their research, and learn from one another new results and problems in a friendly atmosphere. They were 55 researchers, graduate and postdoctoral students from USA, Canada, Poland, Czech Republic, Italy, Spain, Germany, Portugal, Egypt, Senegal, New Zeland, South Africa, Algeria, Turkey, Mexico, Brazil, Uruguay, Indonesia...and France! The interplay between ring and coding theory was emphasized by the very nice and interesting course "Linear codes from the axiomatic point of view" given by Jay Wood. The four invited speakers: Fr\\'ed\\'erique Oggier, Christophe Reutenauer, Angel del Rio and Irfan Siap contributed greatly to the success of this conference. The topics of the Jay Wood's course and the 39 talks presented at the conference are well represented by this special issue of Jacodesmath. They cover pure ring theory such as nil *-clean rings, radical classes or algebras such as Grassman algebras and Steenrod algebras and many papers relating these subjects with coding theory such as MacWilliams extension theorem, dual codes, Lee and Hamming weight. So this volume will be particularly useful for mathematicians at the confluent of these two branches. This meeting was supported by the Laboratoire de Math\\'ematiques de Lens (LML, by different bodies from the Universit\\'e d'Artois (RI, BQR, as well as by a regional organization (the Fédération des Laboratoires de Math´ematiques du Nord pas de Calais. We would like to thank all the participants for their efforts and enthusiasm. A very warm thanks to the colleagues who kindly agreed to referee the papers. Their expertise, promptitude, and professionalism improved the quality of the articles in

  6. Notes for an address

    International Nuclear Information System (INIS)

    Lalonde, Marc.

    1981-06-01

    The government of Canada is involved in the nuclear industry both as a regulator, through the Atomic Energy Control Board, and as a promoter, through Atomic Energy of Canda Ltd. and Eldorado Nuclear. These roles reflect government's desire to ensure that the benefits continue to outweigh the risks of nuclear power. The government is in the process of considering the options available in its policy towards the nuclear industry. Nuclear exports are advantageous to the country, both directly and as a step towards other high-technology exports. A strong safeguards policy is a prerequisite for exports. Nuclear-generated electricity will be one of the substitutes for oil as the country attempts to reduce oil consumption to 10 percent of primary energy use by 1990. Uranium production is one of Canada's strengths. Short-term storage of radioactive wastes is being handled well, and the research program into long-term disposal is making progress. The nuclear industry's attitude towards its waste should serve as a model for other industries. There is much less technological uncertainty about the ability of the CANDU system to meet future energy needs than there is about most other energy sources

  7. Notes for an address

    International Nuclear Information System (INIS)

    MacEachen, A.J.

    1981-06-01

    Over the years, the Canadian government has invested heavily in the nuclear industry. More than $2 billion has been spent on research and development, which now costs upward of $140 million annually. The federal government shared the costs of the first two Pickering reactors and of the heavy water plants. Government loans and international assistance programs have helped the financing of reactor sales to India, Pakistan, Korea, Argentina and Romania. In the late 1940s the federal government purchased Eldorado Nuclear Ltd., and since then has spent over $100 million to help uranium producers. The costs of all these programs have been borne by all Canadian taxpayers, while the considerable benefits have accrued mainly to the provinces with nuclear power programs or significant elements of the nuclear industry: Ontario, and to a lesser extent Quebec, Nova Scotia, and soon New Brunswick. Recent events have dimmed prospects for the nuclear industry, and it appears that more help may be needed. Although the Canadian nuclear industry will not be allowed to die, the financial burdens are going to have to be shared more fairly by those who benefit

  8. Note on the ABC Conjecture

    OpenAIRE

    Carella, N. A.

    2006-01-01

    This note imparts heuristic arguments and theorectical evidences that contradict the abc conjecture over the rational numbers. In addition, the rudimentary datails for transforming this problem into the doimain of equidistribution theory are provided.

  9. Lecture notes on quantum statistics

    NARCIS (Netherlands)

    Gill, R.D.

    2000-01-01

    These notes are meant to form the material for an introductory course on quantum statistics at the graduate level aimed at mathematical statisticians and probabilists No background in physics quantum or otherwise is required They are still far from complete

  10. Alcune Note di Analisi Matematica

    OpenAIRE

    Vasselli, Ezio

    2011-01-01

    Lectures notes (in italian) of some arguments of classical analysis, with exercises. A particular emphasis to functional analysis and elementary operator algebra theory is given, by means of exercises and examples.

  11. EDITORIAL: Publisher's Note

    Science.gov (United States)

    McGlashan, Yasmin

    2006-12-01

    In addition to offering an excellent service to authors, referees and readers of the journal, one of the goals of Plasma Physics and Controlled Fusion (PPCF) is to support the plasma community. We sponsor a number of prizes each year, usually focused on students' contributions to conferences. The poster prize winners from the 33rd EPS Conference on Plasma Physics can be found in Plasma Phys. Control. Fusion 48 B1-B506 along with the invited talks from the conference. Other prizes include: A poster prize at the CCLRC-organised High Power Laser Science Christmas meeting, held in Abingdon, UK, in December 2005. The winning poster was: `Implicit Vlasov Fokker Planck simulations including hydrodynamic ion motion' Christopher Ridgers (Imperial College London). The second prize went to C Cecchitti (Queens University of Belfast), and the third prize to John Howe (University of York). For the first time in 2006 PPCF also sponsored a prize for the best poster presented at the International Workshop on Frontiers of Plasma Physics, held from 21 August-1 September 2006 at the Abdus Salam ICTP, Trieste, Italy. The winner of this prize was Dr Bhaskar Chaudhury (Institute for Plasma Research, Bhat, India), for his work on `Plasma stealth technology: reduction of radar cross section of plasma shrouded objects'. Thank you to everyone who participated in these prizes, and congratulations to the winners and runners-up. I would also like to take this opportunity to thank the authors and referees of PPCF for all their hard work and support in making the journal a success in 2006 and to wish you all a successful 2007.

  12. Editor’s Note

    Directory of Open Access Journals (Sweden)

    Asghar Dadkhah

    2009-04-01

    Full Text Available In rehabilitation, we don't often think about globalization or how what we do may have impact worldwide. Many of us don't have the time, inclination, yet maybe we need to think beyond our borders to pick up a global concept. Apparently the era of globalization is having negative implications for the provision of effective psychiatric rehabilitation programs. The question is whether the impact of globalization can be eliminated or even "neutralized" for the benefit of the patient and the family. The answer is positive. There are solutions like the empowerment of the international movement of families and users of services, with parallel efforts for self-actualization and self-determination of any suffering person. At the community level, the atmosphere could be changed by the systematic implementation of various mental health interventions directed at the modification of the local community's beliefs and attitudes towards the interactions of the mentally ill. We hope the authors discuss these issues in our journal. Regarding Iranian Rehabilitation Journal, beginning with this issue, Volume 7, Number 9, two issues will be published each year, one for spring and one for autumn. In this issue, we begin by exploring in more detail the concept of rehabilitation, then use a historical perspective to examine the changing nature and support for rehabilitation as a correctional goal over time. Our attention next turns to the current treatment programs that are found within the correctional system. Perhaps the most important consideration is whether rehabilitation "works" to reduce the likelihood that offenders will "recidivate". Accordingly, we also review the latest research on the effectiveness of treatment interventions. We conclude this entry with comments on the future of rehabilitation as a correctional goal.

  13. SAFETY INSTRUCTION AND SAFETY NOTE

    CERN Multimedia

    TIS Secretariat

    2002-01-01

    Please note that the SAFETY INSTRUCTION N0 49 (IS 49) and the SAFETY NOTE N0 28 (NS 28) entitled respectively 'AVOIDING CHEMICAL POLLUTION OF WATER' and 'CERN EXHIBITIONS - FIRE PRECAUTIONS' are available on the web at the following urls: http://edms.cern.ch/document/335814 and http://edms.cern.ch/document/335861 Paper copies can also be obtained from the TIS Divisional Secretariat, email: TIS.Secretariat@cern.ch

  14. Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) nees using RAPD and ISSR markers.

    Science.gov (United States)

    Goyal, Arvind Kumar; Pradhan, Sushen; Basistha, Bharat Chandra; Sen, Arnab

    2015-08-01

    Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog's medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants.

  15. PREFACE: Wetting: introductory note

    Science.gov (United States)

    Herminghaus, S.

    2005-03-01

    of very specific and quantitative predictions were put forward which were aimed at direct experimental tests of the developed concepts [9]. Experimentally, wetting phenomena proved to be a rather difficult field of research. While contact angles seem quite easy to measure, deeper insight can only be gained by assessing the physical properties of minute amounts of material, as provided by the molecularly thin wetting layers. At the same time, the variations in the chemical potential relevant for studying wetting transitions are very small, such that system stability sometimes poses hard to solve practical problems. As a consequence, layering transitions in cryogenic systems were among the first to be thoroughly studied [10] experimentally, since they require comparably moderate stability. First-order wetting transitions were not observed experimentally before the early nineties, either in (cryogenic) quantum systems [11,12] or in binary liquid mixtures [13,14]. The first observation of critical wetting, a continuous wetting transition, in 1996 [15] was a major breakthrough [16]. In the meantime, a detailed seminal paper by Pierre Gilles de Gennes published in 1985 [17] had spurred a large number of new research projects which were directed to wetting phenomena other than those related to phase transitions. More attention was paid to non-equilibrium physics, as it is at work when oil spreads over a surface, or a liquid coating beads off (`dewets') from its support and forms a pattern of many individual droplets. This turned out to be an extremely fruitful field of research, and was more readily complemented by experimental efforts than was the case with wetting transitions. It was encouraging to find effects analogous to layering (as mentioned above) in more common systems such as oil films spreading on a solid support [18,19]. Long standing riddles such as the divergence of dissipation at a moving contact line were now addressed both theoretically and experimentally

  16. Micropropagation of caçari under different nutritive culture media ...

    African Journals Online (AJOL)

    Tuoyo Aghomotsegin

    2016-08-17

    Aug 17, 2016 ... 2Brazilian Agricultural Research Corporation (Embrapa), Brazil. ... 4Tropical Research and Education Center, Homestead (TREC-UF), University of .... higher and better use of the nutrients by the explant. ..... affect the establishment and development of the cultures. ..... Teaching, Research and Extension.

  17. Two-stage culture procedure using thidiazuron for efficient micropropagation of Stevia rebaudiana, an anti-diabetic medicinal herb.

    Science.gov (United States)

    Singh, Pallavi; Dwivedi, Padmanabh

    2014-08-01

    Stevia rebaudiana Bertoni, member of Asteraceae family, has bio-active compounds stevioside and rebaudioside which taste about 300 times sweeter than sucrose. It regulates blood sugar, prevents hypertension and tooth decay as well as used in treatment of skin disorders having high medicinal values, and hence there is a need for generating the plant on large scale. We have developed an efficient micropropagation protocol on half strength Murashige and Skoog (MS) media, using two-stage culture procedures. Varying concentrations of cytokinins, i.e., benzylaminopurine, kinetin and thidiazuron (TDZ) were supplemented in the nutrient media to observe their effects on shoot development. All the cytokinins promoted shoot formation, however, best response was observed in the TDZ (0.5 mg/l). The shoots from selected induction medium were sub-cultured on the multiplication media. The media containing 0.01 mg/l TDZ produced maximum number of shoot (11.00 ± 0.40) with longer shoots (7.17 ± 0.16) and highest number of leaves (61.00 ± 1.29). Rooting response was best observed in one-fourth strength on MS media supplemented with indole-3-butyric acid (1.0 mg/l) and activated charcoal (50 mg/l) with (11.00 ± 0.40) number of roots. The plantlets thus obtained were hardened and transferred to the pots with soil and sand mixture, where the survival rate was 80 % after 2 months. Quantitative analysis of stevioside content in leaves of in vivo mother plant and in vitro plantlets was carried out by high performance liquid chromatography. A remarkable increase in stevioside content was noticed in the in vitro-raised plants as compared to in vivo grown plants. The protocol reported here might be useful in genetic improvement and high stevioside production.

  18. EFFICIENT MICROPROPAGATION FROM COTYLEDONARY NODE CULTURES OF COMMIPHORA WIGHTII (ARN. BHANDARI, AN ENDANGERED MEDICINALLY IMPORTANT DESERT PLANT

    Directory of Open Access Journals (Sweden)

    TARUN KANT

    2010-12-01

    Full Text Available Commiphora wightii (Arn. Bhandari, is a medicinal important desert species of the family Burseraceae. It is a well known for its valuable active principle found in its oleo-gum-resin (guggulsterone E and Z, which are used in drugs preparation for lowering the cholesterol level in human body. Due to its overexploitation, poor natural regeneration this valuable plant is on the verge of extinction and thus a IUCN Red listed species. In the present study we report development of an efficient micropropagation protocol from cotyledonary node of Commiphora wightii. Cotyledonary nodes were used as an explants and multiple microshoots were obtained on Murashige & Skoog (MS medium supplemented with 2.68 µM a-Naphthalene acetic acid (NAA and 4.44 µM 6-Benzylamino purine (BAP and on 2.68 µM NAA; 4.44 µM BAP with additives (glutamine 684.2 µM; thiamine 29.65 µM; activated charcoal 0.3% and various other hormonal combinations. Elongation of microshoot was significantly observed on the 2.46 µM Indole-3-butyric acid (IBA and 2.22 µM BAP supplemented MS medium. Efficient rooting was obtained on pretreated microshoot (4.92 µM IBA for 24 hours and followed by transfer to White’s medium without Plant Growth Regulators (PGR and high concentration of activated charcoal (AC. Rooted micro-shoots were transferred to vermiculite and wetted with Hoagland’s solution for primary hardening for 4-5 weeks and then finally transferred to plastic cups containing vermiculite, placed in mist chamber. Plantlets were transferred to soil: FYM 1:1 containing poly-bags, then to green shade house for complete acclimatization and finally transplanted to the experimental field.

  19. Improvement noted after a multifaceted approach to diabetes ...

    African Journals Online (AJOL)

    guidelines by clinicians was also noted in other studies carried out in the United States of ... Health Ethics Review (UHERB) and the Biomedical Research. Ethics Committee ..... results of feasibility study. The DCCT ... there a gap? The South ...

  20. Evaluation of genetic variability in micropropagated propagules of ornamental pineapple [Ananas comosus var. bracteatus (Lindley) Coppens and Leal] using RAPD markers.

    Science.gov (United States)

    Santos, M D M; Buso, G C S; Torres, A C

    2008-10-21

    The objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. The basal culture medium consisted of MS salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/L. The addition of BAP influenced the occurrence of genetic variation revealed using random amplified polymorphic DNA (RAPD) markers. Of a total of 520 primers tested, 44 were selected and amplified; 402 monomorphic bands (97.2%) and 18 polymorphic bands (2.8%) resulted among regenerated plantlets. The polymorphic fragments were produced by 12 primers (OPA-01, OPA-20, OPB-01, OPB-19, OPC-19, OPF-13, OPL-17, OPM-13, OPP-16, OPT-07, OPV-19, and OPX-03). Among the primers that identified polymorphism, OPA-01, OPA-20, OPB-19, OPC-19, OPL-17, OPP-16, and OPX-3 each showed, one polymorphic band and OPF-13 amplified a maximum of three bands. In this study, the RAPD technique was effective in showing the occurrence of somaclonal variations that occur during the micropropagation process of ornamental pineapple cultivation in BAP-supplemented medium, and it is possible to detect the presence of genetic variation in early stages of plant development.

  1. Factors Influencing the Effectiveness of Note Taking on Computer-Based Graphic Organizers

    Science.gov (United States)

    Crooks, Steven M.; White, David R.; Barnard, Lucy

    2007-01-01

    Previous research on graphic organizer (GO) note taking has shown that this method is most effective when the GO is presented to the student partially complete with provided notes. This study extended prior research by investigating the effects of provided note type (summary vs. verbatim) and GO bite size (large vs. small) on the transfer…

  2. An Exploration of Note-Taking in an Online Calculus Course

    Science.gov (United States)

    van de Sande, Carla; Abramson, Jay; Judson-Garcia, Julia

    2017-01-01

    Taking notes is a popular activity for students attending instructional lectures and has been linked to achievement. Research on note-taking has shown that the contents and usefulness of student notes depends on several factors, including the pace of the lecture and the presence of organizational and selection cues. However, this research has…

  3. Research

    African Journals Online (AJOL)

    ebutamanya

    2016-04-22

    Apr 22, 2016 ... Papulosquamous disorders, Infectious disorders, Connective tissue diseases, Bullous disorders, ... surgical pathology specimen received within the study period. The modal age ... enable appropriate management. ... basic clinical data including age, gender were noted. ... Confidentiality of the identity of the.

  4. Technical note on drainage systems

    DEFF Research Database (Denmark)

    Bentzen, Thomas Ruby

    This technical note will present simple but widely used methods for the design of drainage systems. The note will primarily deal with surface water (rainwater) which on a satisfactorily way should be transport into the drainage system. Traditional two types of sewer systems exist: A combined system......, where rainwater and sewage is transported in the same pipe, and a separate system where the two types of water are transported in individual pipe. This note will only focus on the separate rain/stormwater system, however, if domestic sewage should be included in the dimensioning procedure, it......’s not major different than described below - just remember to include this contribution for combined systems where the surface water (rain) and sewage are carried in the same pipes in the system and change some of the parameters for failure allowance (this will be elaborated further later on). The technical...

  5. Acclimatization and leaf anatomy of micropropagated fig plantlets Aclimatização e anatomia foliar de plântulas de figo micropropagadas

    Directory of Open Access Journals (Sweden)

    Chrystiane Fráguas Chirinéa

    2012-12-01

    Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.A sobrevivência de plantas micropropagadas durante e após a aclimatização é um processo limitante para o estabelecimento de plantas. Poucas informações são encontradas de como a anatomia de órgãos vegetativos de Ficus carica pode ser afetada pelas condições de cultivo e aclimatização. O presente trabalho teve por objetivo estudar os efeitos do tempo em meio de cultura e substratos durante a aclimatização de plântulas de figueira produzidas in vitro e cultivadas no campo. Plântulas multiplicadas in vitro foram separadas e transferidas para meio WPM, onde foram mantidas por 0; 15; 30; 45 e 60 dias. Diferentes

  6. Gaz de France. Operation note

    International Nuclear Information System (INIS)

    2003-01-01

    This note was published for the public at the occasion of the admission to Euronext's Eurolist of the existing shares that make the capital of Gaz de France company, the French gas utility. The note gives some informations about Gaz de France activity, and about its strategy of development in the European gas market. Then it describes the offer relative to the opening of Gaz de France capital. Some selected financial data and some precision about the risk factors and the management of the company complete the document. (J.S.)

  7. Churchill on Stalin: A note.

    NARCIS (Netherlands)

    Ellman, M.J.

    2006-01-01

    The purpose of this note is to draw attention to two limitations of Churchill's war memoirs as a source of accurate information about Stalin, his views ans actions. they concern, first Stalin's alleged remarks in 1942 about collectivisation, and second Stalin's allleged response to Churchill's

  8. Lecture notes on ideal magnetohydrodynamics

    International Nuclear Information System (INIS)

    Goedbloed, J.P.

    1983-03-01

    Notes, prepared for a course of lectures held at the Instituto de Fisica, Universidade Estadual de Campinas, Brazil (June-August 1978). An extensive theoretical treatment of the behaviour of hot plasmas caught in equations and mathematical models is presented in 12 chapters

  9. Developing INDCs: a guidance note

    DEFF Research Database (Denmark)

    Bakkegaard, Riyong Kim; Bee, Skylar; Naswa, Prakriti

    needs and low capability, would need means of implementation (MoI) for adaptation and to take ambitious mitigation actions. Developing countries would include MoI needs in the context of mitigation and adaptation. The note explains briefly how countries can identify their unconditional contributions....

  10. A note on hypoplastic yielding

    OpenAIRE

    Nader, José Jorge

    2010-01-01

    This note discusses briefly the definition of yield surface in hypoplasticity in connection with the physical notion of yielding. The relation of yielding with the vanishing of the material time derivative of the stress tensor and the vanishing of the corotational stress rate is investigated.

  11. The future of NOTES from the conservative point of view.

    Science.gov (United States)

    Kano, Nobuyasu

    2009-01-01

    Recently, the unfamiliar term "natural orifice transluminal endoscopic surgery (NOTES)" appeared in my field. Actually, I am hesitant to accept this technique in my surgical practice. In this paper, I will review some references and try to establish my position toward NOTES. The author has been skeptical and ironical about the clinical potentiality of NOTES since hearing the presentation about NOTES for the first time. I have been concerned about making a puncture in the gastrointestinal tract as an old surgeon who believes that intestinal injury must not occur during surgery. However, recent advances in the research of NOTES are changing my stubborn belief. What I have to do is to avoid interrupting or disturbing young surgeons' challenges to develop NOTES. I remember that some senior surgeons were against us when we started laparoscopic surgery around 1990. Senior surgeons and physicians must be generous, considerate, helpful and supportive to our followers. I have been enthusiastic about the development and spread of laparoscopic surgery since 1987 and have been doing various surgical procedures myself, including those involving the biliary tract, pancreas, spleen, upper and lower gastrointestinal tract, adrenal, kidney and gynecologic organs. Recently, the unfamiliar term "NOTES" appeared in my field. Actually, I am hesitant to accept this technique in my surgical practice. In this paper I will review some references and try to establish my position toward NOTES.

  12. Crescimento de mudas micropropagadas de bananeira micorrizadas em diferentes recipientes Growth of mycorrhizal micropropagated banana plantlets in different recipients

    Directory of Open Access Journals (Sweden)

    Patrícia Lopes Leal

    2005-04-01

    Full Text Available Realizou-se um experimento em casa de vegetação, com o objetivo de avaliar a utilização de diferentes recipientes e a eficiência da inoculação com fungo micorrízico arbuscular (FMA, Glomus clarum, no crescimento de mudas micropropagadas de bananeira. O delineamento experimental foi o de blocos casualizados, no esquema fatorial 2x2, sendo 2 tratamentos microbiológicos: Glomus clarum e controle; e 2 recipientes: blocos prensados e tubetes, com 6 repetições. O substrato utilizado para a confecção dos blocos e para o enchimento dos tubetes foi constituído por uma mistura de materiais orgânicos (bagaço de cana + torta de filtro de usina açucareira e vermiculita. Mudas de bananeira produzidas em blocos prensados e inoculadas com o FMA apresentaram melhores resultados quando comparadas com as produzidas nos tubetes, com incrementos na altura, na produção de matéria seca da parte aérea e no acúmulo de N, P e K de 90%, 829%, 2774%, 249% e 403%, respectivamente.An experiment under greenhouse was carried out in order to evaluate different recipients and the inoculation with arbuscular mycorrhizal fungus (AMF, Glomus clarum, on the growth of micropropagated banana plantlets. A random block design in a factorial scheme 2x2 (two microbiological treatments: Glomus clarum and control; two types of recipients: pressed blocks and plastic tubes, with six repetitions, was used. The substrate utilized to construct the pressed blocks and to fill the tubes was constituted by a mixture of organic residue (sugarcane bagasse and filter cake and vermiculite. Banana plantlets produced in pressed blocks and inoculated with AMF let to better results than their counterparts grown in tubes, increasing the height, shoot dry weight, contents of N, P and K in 90%, 829%, 2774%, 249% and 403%, respectively.

  13. Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp

    Czech Academy of Sciences Publication Activity Database

    Roux, N.; Doležel, Jaroslav; Swennen, R.; Zapata-Arias, F.

    2001-01-01

    Roč. 66, - (2001), s. 189-197 ISSN 0167-6857 R&D Projects: GA ČR GV521/96/K117 Institutional research plan: CEZ:AV0Z5038910 Keywords : chimera * flow cytometry * in vitro tissue culture Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.631, year: 2001

  14. Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

    Czech Academy of Sciences Publication Activity Database

    Pospíšilová, Jana; Synková, Helena; Haisel, Daniel; Baťková, Petra

    2009-01-01

    Roč. 53, č. 1 (2009), s. 11-20 ISSN 0006-3134 R&D Projects: GA ČR GA522/07/0227 Institutional research plan: CEZ:AV0Z50380511 Keywords : carotenoids * chlorophyll contents * chlorophyll fluorescence Subject RIV: ED - Physiology Impact factor: 1.656, year: 2009

  15. European Science Notes. Volume 40, Number 1.

    Science.gov (United States)

    1986-01-01

    Mass Spectrometry mers and copolymers of polyacrylate salt series edited by Professor J.F.J. Todd latex) rather than an inorganic or or- (University...changes in the popu- cy with two potassium dihydrogen phos- lation of a vibrational manifold were phate (KDP) crystals. Following a fil- determined by...AD-A162 235 EUROPEAN SCIENCE NOTES VOLUME 48 NUMBER I(U) OFFICE OF i/1 NAVAL RESEARCH LONDON (ENGLAND) L E SHAFFER JAN 86 UNCLASSIFIED F/G 5/2

  16. NEW CYTOKINlNS - META-METHOXYTOPOLlNS IN MICROPROPAGATION OF COTINUS COGGYGRIA SCOP. 'ROYAL PURPLE'

    Czech Academy of Sciences Publication Activity Database

    Podwyszynska, M.; Wegrzynowicz-Lesiak, E.; Doležal, Karel; Krekule, Jan; Strnad, Miroslav; Saniewski, M.

    2012-01-01

    Roč. 12, č. 4 (2012), s. 220-228 ISSN 1311-9109 Grant - others:GA MŠk(CZ) ED0007/01/01 Program:ED Institutional research plan: CEZ:AV0Z50380511 Keywords : BA-derivatives * early senescence * in vitro propagation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.492, year: 2012 http://www.journal-pop.org/2012_12_4_220-228.html

  17. Micropropagation of ornamental species Petunia grandiflora and Dianthus chinensis x barbatus

    OpenAIRE

    Velesanova, Ivana; Trajkova, Fidanka; Koleva Gudeva, Liljana

    2017-01-01

    Petunia (Petunia sp.) and pink dianthus (Dianthus sp.) are important ornamental plants which are grown on balconies, in flower gardens, parks and other during summer period. Approximately 156 ornamental species are cultivated via tissue culture in different commercial laboratories worldwide. In vitro plant culture is one of the key tools in plant biotechnology that utilizes the totipotency of plant cells. In this research the influence of different concentrations and combinations of BA, GA...

  18. Micropropagação do porta-enxerto de videira '420-A' Micropropagation of '420-A' grapevine rootstock

    Directory of Open Access Journals (Sweden)

    Pryscilla Menarin Dzazio

    2002-12-01

    meio MS/2 e aclimatização em substrato vermiculita ou Plantmax®.The objective of this work was to establish a protocol for the rootstock of 420-A micropropagation. The establishment of the cultures was accomplished with nodal segments, whose source of explants was the budding of woody stakes stored under refrigeration. In the initial cultivation were tested: the effect of 6-benzilaminopurine and kinetin with 0, 1, 5 and 10 µM concentrations, different culture medium (MS, NN and WPM and dilutions of the basic medium (MS, MS/2, MS/4 and MS/8. In the alongation and multiplication steps were tested the following culture medium: MS, MS/2, NN and WPM. In the rooting were tested: the MS/2 culture medium with or without activated coal (1gL-1. In the acclimatization were tested the substrate vermiculite, Plantmax and carbonized rice hulls. The kinetin didn't present effect on the budding and the growth of the nodal segments. BAP already promoted an increase in the number of sprouts per explant. The increase in the concentration of BAP reduced the number of leaves emitted by explant and increased the vitrification symptoms, being the best results obtained with 1 µM of BAP. In the initial cultivation the MS medium culture with the normal concentration of salts allowed the largest growth of the buddings. The dilutions of the MS medium in ¼ and 1/8 showed very harmful to the development of the rootstock '420-A', being quite harmed the growth of the shoots after the first subcultivation. During the multiplication the medium MS/2 showed more appropriate. The roots happened naturally during the multiplication, being unnecessary the use of activated coal in the culture medium. The acclimatization was accomplished with success in a misty camera, being obtained high survival rates in vermiculite (95,8% and Plantmax® (87%. It is concluded that the rootstock '420-A' can be micropropagated by initial cultivation of nodal segments in a culture medium of MS+1µM of BAP, multiplication

  19. Performance of the diffraction grating on a bank note: the experience with the Australian Commemorative note

    Science.gov (United States)

    Hardwick, Bruce A.

    1990-04-01

    In January 1988, we at the Note Printing Branch first released to the Reserve Bank a polymer based banknote containing an Optically Variable Device (OVD), a diffraction grating. This banknote was the culmination of many years of Research and Development effort, and represented a very significant departure from conventional banknote technology in many ways. It was a test bed. We are very pleased with its performance.

  20. Notes on instrumentation and control

    CERN Document Server

    Roy, G J

    2013-01-01

    Notes on Instrumentation and Control presents topics on pressure (i.e., U-tube manometers and elastic type gauges), temperature (i.e. glass thermometer, bi-metallic strip thermometer, filled system thermometer, vapor pressure thermometer), level, and flow measuring devices. The book describes other miscellaneous instruments, signal transmitting devices, supply and control systems, and monitoring systems. The theory of automatic control and semi-conductor devices are also considered. Marine engineers will find the book useful.

  1. Notes on modeling and simulation

    Energy Technology Data Exchange (ETDEWEB)

    Redondo, Antonio [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-03-10

    These notes present a high-level overview of how modeling and simulation are carried out by practitioners. The discussion is of a general nature; no specific techniques are examined but the activities associated with all modeling and simulation approaches are briefly addressed. There is also a discussion of validation and verification and, at the end, a section on why modeling and simulation are useful.

  2. Note-Taking Skills of Middle School Students with and without Learning Disabilities

    Science.gov (United States)

    Boyle, Joseph R.

    2010-01-01

    For middle school students with learning disabilities (LD), one major component of learning in content area classes, such as science, involves listening to lectures and recording notes. Lecture learning and note-taking are critical skills for students to succeed in these classes. Despite the importance of note-taking skills, no research has been…

  3. The pen is mightier than the keyboard: advantages of longhand over laptop note taking.

    Science.gov (United States)

    Mueller, Pam A; Oppenheimer, Daniel M

    2014-06-01

    Taking notes on laptops rather than in longhand is increasingly common. Many researchers have suggested that laptop note taking is less effective than longhand note taking for learning. Prior studies have primarily focused on students' capacity for multitasking and distraction when using laptops. The present research suggests that even when laptops are used solely to take notes, they may still be impairing learning because their use results in shallower processing. In three studies, we found that students who took notes on laptops performed worse on conceptual questions than students who took notes longhand. We show that whereas taking more notes can be beneficial, laptop note takers' tendency to transcribe lectures verbatim rather than processing information and reframing it in their own words is detrimental to learning. © The Author(s) 2014.

  4. Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb.

    Science.gov (United States)

    Nikam, Tukaram D; Ghorpade, Ravi P; Nitnaware, Kirti M; Ahire, Mahendra L; Lokhande, Vinayak H; Chopra, Arvind

    2013-01-01

    Micropropagation through cotyledonary and leaf node and boswellic acid production in stem callus of a woody medicinal endangered tree species Boswellia serrata Roxb. is reported. The response for shoots, roots and callus formation were varied in cotyledonary and leafy nodal explants from in vitro germinated seeds, if inoculated on Murshige and Skoog's (MS) medium fortified with cytokinins and auxins alone or together. A maximum of 8.0 ± 0.1 shoots/cotyledonary node explant and 6.9 ± 0.1 shoots/leafy node explants were produced in 91 and 88 % cultures respectively on medium with 2.5 μM 6-benzyladenine (BA) and 200 mg l(-1) polyvinylpyrrolidone (PVP). Shoots treated with 2.5 μM IBA showed the highest average root number (4.5) and the highest percentage of rooting (89 %). Well rooted plantlets were acclimatized and 76.5 % of the plantlets showed survival upon transfer to field conditions. Randomly amplified polymorphic DNA (RAPD) analysis of the micropropagated plants compared with mother plant revealed true-to-type nature. The four major boswellic acid components in calluses raised from root, stem, cotyledon and leaf explants were analyzed using HPLC. The total content of four boswellic acid components was higher in stem callus obtained on MS with 15.0 μM IAA, 5.0 μM BA and 200 mg l(-1) PVP. The protocol reported can be used for conservation and exploitation of in vitro production of medicinally important non-steroidal anti-inflammatory metabolites of B. serrata.

  5. Micropropagation effect on the anti-carcinogenic activitiy of polyphenolics from Mexican oregano (Poliomintha glabrescens Gray) in human colon cancer cells HT-29.

    Science.gov (United States)

    García-Pérez, Enrique; Noratto, Giuliana D; García-Lara, Silverio; Gutiérrez-Uribe, Janet A; Mertens-Talcott, Susanne U

    2013-06-01

    Phenolic extracts obtained from spices are known to have anti-carcinogenic activities but little is known about the effect of micropropagation on these beneficial effects. The main objective of this study was to evaluate the cytotoxic activity of flavonoid-enriched extracts (FEE) from the leaves of wild (WT), in vitro (IN), and ex vitro (EX) grown oregano plants in colon cancer cells HT-29 and the non-cancer cells CCD-18Co. Cell proliferation of HT-29 cells was reduced to 50 % by WT, IN, and EX at concentrations of 4.01, 1.32, and 4.84 mg of gallic acid equivalents (GAE)/L, respectively. In contrast, in CCD-18Co cells, higher concentrations were required for the same cytotoxic effect. At 6 mg GAE/L, WT and IN reduced the production of reactive oxygen species (ROS) of lipopolysaccharides (LPS)-stimulated control cells to 59.89 and 59.43 %, respectively, and EX to 73.89 %. The mRNA of Caspase-3 was increased 1.53-fold when cells were treated with 4 mg GAE/L of IN extract, and tumor necrosis factor receptor superfamily, member 6 (FAS), and BCL2-associated X protein (BAX) mRNA increased 2.55 and 1.53 fold, respectively. Results on protein expression corroborated the apoptotic effects with a significant decrease of B-cell lymphoma 2 (BCL2) expression for all treatments but more remarkable for EX that also showed the most intense signal of BAX. Overall, FEE extracts derived from micropropagation had increased pro-apoptotic effects, however extracts from the in vitro plants produced more efficacy at the transcriptional level while extracts from the ex vitro plant were superior at the traductional level.

  6. Effect of explant origin and different growth regulators on micropropagation of Pistacia atlantica ssp. mutica

    Directory of Open Access Journals (Sweden)

    Ali-Ashraf Mehrabi

    2015-06-01

    Full Text Available Propagation of wild pistachio as a multipurpose woody species is a hard and tedious task. In this research, an effective in vitro protocol was developed for rapid proliferation of wild pistachio (Pistacia atlantica ssp. mutica in MS medium supplemented with B5 vitamins and different growth regulators. Rooting of plantlets was tested by two treatments containing Rhizopon and IBA in ex vitro. With respect to the results, the nodal segments explants, produced the highest shoot frequency, leaf frequency and the tallest shoots. On the other hand, the tallest shoots were generated from shoot tip explant and medium containing of TDZ plus IAA. Both treatments (Rhizopon and IBA led to a remarkable increase in the number of roots, root length and rooting percentage compared to the control. These results may be applied for rapid proliferation to spread the pistachio trees and shrubs that are difficult and time consuming.

  7. The Impact of Note Taking On the Improvement of Listening Comprehension of Iranian EFL Learners

    OpenAIRE

    Mohammad Zohrabi; Farzaneh Esfandyari

    2014-01-01

    Note taking is a popular and operative strategy which increases the students? ability to remember, comprehend, and keep the material in mind. Nowadays, it is very common for teachers to use the note taking strategy in EFL listening classes due to the fact that taking notes can help students catch the main points easily and in turn promote their listening comprehension effectively. However, it creates areas of concerns for some researchers about whether taking notes is effective for students t...

  8. IN VITRO MICROPROPAGATION OF ORCHID Dendrobium heterocarpum Lindl. WITH DIFFERENT MEDIUM

    Directory of Open Access Journals (Sweden)

    Yuli Setiawati

    2015-03-01

    Full Text Available Dendrobium heterocarpum Lindl. is one of the orchid species which is often seeked. It is because this species has a mystique appearance and special fragrance. The research aimed to observe the growth and developing responses of protocorm like bodies (PLBs of D. heterocarpum after 12 weeks in three different medium, i.e. Murashige and Skoog (MS, Kursor C and Western 3 (W3. The D. heterocarpum seeds were planted in MS, Kursor C and W3 medium. Qualitatively the orchid growth were observe including growth score and colour score. The result showed that growing time of MS was 4 weeks, a week faster than the growing time on Kursor C and W3 medium. The growing phase on MS medium and Kursor C medium reached phase 1 whereas on W3 medium reached phase 2. PLB’s colour after 12 weeks on MS medium reached phase E, strong yellowish green phase (142A. Meanwhile, on Kursor C and W3 was on phase D, moderate yellowish green (143D.

  9. Revising Lecture Notes: How Revision, Pauses, and Partners Affect Note Taking and Achievement

    Science.gov (United States)

    Luo, Linlin; Kiewra, Kenneth A.; Samuelson, Lydia

    2016-01-01

    Note taking has been categorized as a two-stage process: the recording of notes and the review of notes. We contend that note taking might best involve a three-stage process where the missing stage is revision. This study investigated the benefits of revising lecture notes and addressed two questions: First, is revision more effective than…

  10. Lecture notes for criticality safety

    International Nuclear Information System (INIS)

    Fullwood, R.

    1992-03-01

    These lecture notes for criticality safety are prepared for the training of Department of Energy supervisory, project management, and administrative staff. Technical training and basic mathematics are assumed. The notes are designed for a two-day course, taught by two lecturers. Video tapes may be used at the options of the instructors. The notes provide all the materials that are necessary but outside reading will assist in the fullest understanding. The course begins with a nuclear physics overview. The reader is led from the macroscopic world into the microscopic world of atoms and the elementary particles that constitute atoms. The particles, their masses and sizes and properties associated with radioactive decay and fission are introduced along with Einstein's mass-energy equivalence. Radioactive decay, nuclear reactions, radiation penetration, shielding and health-effects are discussed to understand protection in case of a criticality accident. Fission, the fission products, particles and energy released are presented to appreciate the dangers of criticality. Nuclear cross sections are introduced to understand the effectiveness of slow neutrons to produce fission. Chain reactors are presented as an economy; effective use of the neutrons from fission leads to more fission resulting in a power reactor or a criticality excursion. The six-factor formula is presented for managing the neutron budget. This leads to concepts of material and geometric buckling which are used in simple calculations to assure safety from criticality. Experimental measurements and computer code calculations of criticality are discussed. To emphasize the reality, historical criticality accidents are presented in a table with major ones discussed to provide lessons-learned. Finally, standards, NRC guides and regulations, and DOE orders relating to criticality protection are presented

  11. Lecture Notes on Topics in Accelerator Physics

    Energy Technology Data Exchange (ETDEWEB)

    Chao, Alex W.

    2002-11-15

    These are lecture notes that cover a selection of topics, some of them under current research, in accelerator physics. I try to derive the results from first principles, although the students are assumed to have an introductory knowledge of the basics. The topics covered are: (1) Panofsky-Wenzel and Planar Wake Theorems; (2) Echo Effect; (3) Crystalline Beam; (4) Fast Ion Instability; (5) Lawson-Woodward Theorem and Laser Acceleration in Free Space; (6) Spin Dynamics and Siberian Snakes; (7) Symplectic Approximation of Maps; (8) Truncated Power Series Algebra; and (9) Lie Algebra Technique for nonlinear Dynamics. The purpose of these lectures is not to elaborate, but to prepare the students so that they can do their own research. Each topic can be read independently of the others.

  12. Lecture Notes on Topics in Accelerator Physics

    International Nuclear Information System (INIS)

    Chao, Alex W.

    2002-01-01

    These are lecture notes that cover a selection of topics, some of them under current research, in accelerator physics. I try to derive the results from first principles, although the students are assumed to have an introductory knowledge of the basics. The topics covered are: (1) Panofsky-Wenzel and Planar Wake Theorems; (2) Echo Effect; (3) Crystalline Beam; (4) Fast Ion Instability; (5) Lawson-Woodward Theorem and Laser Acceleration in Free Space; (6) Spin Dynamics and Siberian Snakes; (7) Symplectic Approximation of Maps; (8) Truncated Power Series Algebra; and (9) Lie Algebra Technique for nonlinear Dynamics. The purpose of these lectures is not to elaborate, but to prepare the students so that they can do their own research. Each topic can be read independently of the others

  13. Notes on elementary particle physics

    CERN Document Server

    Muirhead, William Hugh

    1972-01-01

    Notes of Elementary Particle Physics is a seven-chapter text that conveys the ideas on the state of elementary particle physics. This book emerged from an introductory course of 30 lectures on the subject given to first-year graduate students at the University of Liverpool. The opening chapter deals with pertinent terminologies in elementary particle physics. The succeeding three chapters cover the concepts of transition amplitudes, probabilities, relativistic wave equations and fields, and the interaction amplitude. The discussion then shifts to tests of electromagnetic interactions, particul

  14. Utilization of the biorreactor of imersion by bubbles at the micropropagation of Ananas comosus L. Merril

    Directory of Open Access Journals (Sweden)

    Gessiel Newton Scheidt

    2009-11-01

    Full Text Available The research for new techniques of in vitro cultivation is being object of many studies around the world, in order to optimize and decrease production costs of seedlings with agronomical interest. The main goal of this work was to compare different systems of in vitro cultivations using Ananas comosus L. Merril. So, the in vitro growth of the plantlets was promoted in two different bioreactors: Bioreactor of Immersion by Bubbles (B.I.B.® and the Reactor of Temporary Immersion (R.I.T.A.® with immersion cycle every 2 hours for 15 minutes and the traditional system in flasks with 200 mL. All cultivation systems used the MS liquid nutritive solution, supplemented with BAP (1 mgL-1, ANA (0.25 mgL-1, sucrose (30 gL-1 and Tween 20® (0.5 µL. The pH was adjusted to 5.8 and sterilized at 120°C for 15 minutes. The cultures were kept into a growth room during 30 days, with controlled temperature of 25±2°C, under white cold light (46.8 µmol.m-2.s-1, with photoperiod of 16 hours. The experimental design used was randomized, with three treatments, three repetitions and ten plants each stage. Among the evaluated systems, the BIB® presented the best results for the tested variables, mainly the total number of shoots, being 2.3 e 3.1 times superior when compared with the system R.I.T.A.® and the traditional consecutively. So the system of immersion by bubbles turns into an effective equipment to produce seedlings of pineapple in large scale.A busca por novas técnicas de cultivo in vitro vem sendo amplamente estudadas, visando otimizar e baixar o custo de produção das mudas que tenham interesse agronômico. O objetivo deste trabalho foi comparar diferentes sistemas de cultivo in vitro de Ananas comosus L. Merril. Para tanto, o crescimento in vitro de plântulas foi promovido em sistemas de biorreatores (B.I.B.® e R.I.T.A.® com ciclo de imersão a cada 2 horas por 15 minutos e o sistema tradicional em frascos de 200 mL. Em todos os sistemas de

  15. Debriefing Note Secondary Education Support Programme

    DEFF Research Database (Denmark)

    Webster, Neil; Vagnby, Bo Hellisen; Thomsen, Thomas J.

    Debriefing note regarding joint programming for the Secondary Education Support Programme (2003- 2007). The note specifies preparation of SIP Physical Guidelines; Training needs assessment for Physical School Status and Rapid Technical Assessments; SIP/DEP preparation; Selection criteria...

  16. Note-Making in Social Studies.

    Science.gov (United States)

    Fowler, Robert W.

    1985-01-01

    Note-making is one excellent method for helping students retain important points made by the teacher. Techniques that elementary and secondary social studies teacher can use to teach note-making skills are described. (RM)

  17. Taking notes as an interactive process

    OpenAIRE

    Hornig, Wolfgang

    1984-01-01

    Taking notes as an interactive process : how to improve students´ notes / Hornig W. ; Nowak, J. - In: Nowak, Johann: Textverstehen und Textrekonstruktion in Vorlesungen. - Augsburg : HDZ, 1984. - S. 227-253. - (Augsburger Studien zur Hochschuldidaktik ; 12)

  18. The new bank note distribution system

    OpenAIRE

    Gerrit Bilkes

    1997-01-01

    In this article, the author outlines the recent changes made to the way Canada's bank notes are distributed. The new system allows financial institutions to exchange notes directly with one another at designated points across the country, rather than through Bank of Canada agencies, as was previously the case. The institutions communicate with the Bank of Canada through a computerized inventory-management system. Two Bank of Canada operations centres monitor note quality and supply new notes ...

  19. What Predicts Skill in Lecture Note Taking?

    Science.gov (United States)

    Peverly, Stephen T.; Ramaswamy, Vivek; Brown, Cindy; Sumowski, James; Alidoost, Moona; Garner, Joanna

    2007-01-01

    Despite the importance of good lecture notes to test performance, very little is known about the cognitive processes that underlie effective lecture note taking. The primary purpose of the 2 studies reported (a pilot study and Study 1) was to investigate 3 processes hypothesized to be significantly related to quality of notes: transcription…

  20. Lecture notes on diophantine analysis

    CERN Document Server

    Zannier, Umberto

    2014-01-01

    These lecture notes originate from a course delivered at the Scuola Normale in Pisa in 2006. Generally speaking, the prerequisites do not go beyond basic mathematical material and are accessible to many undergraduates. The contents mainly concern diophantine problems on affine curves, in practice describing the integer solutions of equations in two variables. This case historically suggested some major ideas for more general problems. Starting with linear and quadratic equations, the important connections with Diophantine Approximation are presented and Thue's celebrated results are proved in full detail. In later chapters more modern issues on heights of algebraic points are dealt with, and applied to a sharp quantitative treatment of the unit equation. The book also contains several Supplements, hinted exercises and an Appendix on recent work on heights.

  1. Lecture notes on wavelet transforms

    CERN Document Server

    Debnath, Lokenath

    2017-01-01

    This book provides a systematic exposition of the basic ideas and results of wavelet analysis suitable for mathematicians, scientists, and engineers alike. The primary goal of this text is to show how different types of wavelets can be constructed, illustrate why they are such powerful tools in mathematical analysis, and demonstrate their use in applications. It also develops the required analytical knowledge and skills on the part of the reader, rather than focus on the importance of more abstract formulation with full mathematical rigor.  These notes differs from many textbooks with similar titles in that a major emphasis is placed on the thorough development of the underlying theory before introducing applications and modern topics such as fractional Fourier transforms, windowed canonical transforms, fractional wavelet transforms, fast wavelet transforms, spline wavelets, Daubechies wavelets, harmonic wavelets and non-uniform wavelets. The selection, arrangement, and presentation of the material in these ...

  2. Micropropagation of Lavandula spp.

    Science.gov (United States)

    Gonçalves, Sandra; Romano, Anabela

    2013-01-01

    Lavandula species are some of the most popular ornamental and medicinal plants with great economic values. These species are vegetative propagated by stem cuttings. However, the poor rooting ability and vulnerability of plantlets to contamination are major limiting factors for propagation. In vitro culture methods are suitable to overcome these limitations. This chapter describes protocols for in vitro propagation of Lavandula viridis L'Hér and Lavandula vera DC. Nodal shoot proliferation of L. viridis and plant regeneration from leaf-derived callus of L. vera by an "open culture system" are highlighted.

  3. Needs analysis for developing a virtual-reality NOTES simulator.

    Science.gov (United States)

    Sankaranarayanan, Ganesh; Matthes, Kai; Nemani, Arun; Ahn, Woojin; Kato, Masayuki; Jones, Daniel B; Schwaitzberg, Steven; De, Suvranu

    2013-05-01

    INTRODUCTION AND STUDY AIM: Natural orifice translumenal endoscopic surgery (NOTES) is an emerging surgical technique that requires a cautious adoption approach to ensure patient safety. High-fidelity virtual-reality-based simulators allow development of new surgical procedures and tools and train medical personnel without risk to human patients. As part of a project funded by the National Institutes of Health, we are developing the virtual transluminal endoscopic surgery trainer (VTEST) for this purpose. The objective of this study is to conduct a structured needs analysis to identify the design parameters for such a virtual-reality-based simulator for NOTES. A 30-point questionnaire was distributed at the 2011 National Orifice Surgery Consortium for Assessment and Research meeting to obtain responses from experts. Ordinal logistic regression and the Wilcoxon rank-sum test were used for analysis. A total of 22 NOTES experts participated in the study. Cholecystectomy (CE, 68 %) followed by appendectomy (AE, 63 %) (CE vs AE, p = 0.0521) was selected as the first choice for simulation. Flexible (FL, 47 %) and hybrid (HY, 47 %) approaches were equally favorable compared with rigid (RI, 6 %) with p virtual NOTES simulator in training and testing new tools for NOTES were rated very high by the participants. Our study reinforces the importance of developing a virtual NOTES simulator and clearly presents expert preferences. The results of this analysis will direct our initial development of the VTEST platform.

  4. Implications of observing and writing field notes through different lenses

    Directory of Open Access Journals (Sweden)

    Hellesø R

    2015-04-01

    Full Text Available Ragnhild Hellesø,1 Line Melby,1 Solveig Hauge21Department of Nursing Science, Institute of Health and Society, Faculty of Medicine, University of Oslo, Oslo, Norway; 2Faculty of Health and Social Studies, Telemark University College, Porsgrunn, NorwayBackground: From a philosophy of science perspective, the literature has posited that different research approaches influence field studies. Studies addressing interdisciplinary research have focused on the challenges of organizing and running interdisciplinary teams, cultural differences between and within disciplines, and constraints in conducting interdisciplinary research. Studies exploring and discussing the process and outcome of transferring observations to notes from an interdisciplinary point of view are not identified. The aim of this paper is to explore the characteristics of field notes created by researchers representing different disciplines and experiences.Methods: A case study using a modified dynamic observation method was employed. The analyses were initiated by a researcher who had not been involved in the data collection. The field notes were analyzed using three main steps.Results: The structures of both researchers' field notes were characterized by similarities in their descriptions, but the notes' foci and analytical levels differed.Conclusion: The findings contribute new insights concerning the execution of interdisciplinary observational studies. Our findings demonstrate that entering the field with different lenses produced richer and more varied data, providing a broader platform from which to discuss and interpret a study's findings. From a theoretical point of view, the findings enable a more nuanced discussion and a conceptual elaboration regarding how observational approaches should be pursued in future studies. On a practical level, the findings show that even if the researchers agree on what the overall focus in the observations should be, differences can occur in

  5. Sealing and explant types on the mangaba micropropagation Tipo de vedação e explantes na micropropagação de mangabeira

    Directory of Open Access Journals (Sweden)

    Aline de Jesus Sá

    2012-08-01

    Full Text Available In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L-1 IAA and 1 mg L-1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro cultivation. The best types of flask sealing for the establishment phase were the PVC film and Para-film® and for the first subculture the Para-film® seal. In the second subculture the PVC film and Para-film® seals promoted the best growth. The median and basal nodal segments presented the best performance in the first subculture. No significant effect of explant type was observed in the second subculture. The ideal subculture interval in the establishment phase and the first and second subcultures is 50 days.Na micropropagação, especialmente para mangaba, variedade botânica da árvore do Nordeste do Brasil, aspectos limitantes, como acúmulo de etileno no recipiente de cultivo e perda de vigor em subculturas têm sido observados. Neste estudo, objetivou-se avaliar o conhecimento técnico e científico da propagação in vitro de mangabeira, variedade botânica do Nordeste do Brasil, e melhorar o protocolo de micropropagação, o melhor tipo de vedação frasco e explante em diferentes etapas. Para a fase de estabelecimento e para as subculturas primeiro e segundo, foi utlizado o meio MS com 3% de sacarose e agar 0,6%, suplementado com 1 mg L-1 de AIA e 1 mg L-1 de BAP. As avaliações foram

  6. Research

    African Journals Online (AJOL)

    ebutamanya

    2015-03-02

    Mar 2, 2015 ... Joseph Daniels1,&, Ruth Nduati1,2, James Kiarie1,3, Carey Farquhar1,4,5 .... or basic science research career (Socio-Behavioral Research, .... a research environment that supports knowledge sharing to develop research ...

  7. The Impact of Guided Notes on Post-Secondary Student Achievement: A Meta-Analysis

    Science.gov (United States)

    Larwin, Karen H.; Larwin, David A.

    2013-01-01

    The common practice of using of guided notes in the post-secondary classroom is not fully appreciated or understood. In an effort to add to the existing research about this phenomenon, the current investigation expands on previously published research and one previously published meta-analysis that examined the impact of guided notes on…

  8. The Impact of Structured Note Taking Strategies on Math Achievement of Middle School Students

    Science.gov (United States)

    Wilkinson, Gregory Ashley

    2012-01-01

    Student math achievement continues to be a national, state, and local concern. Research suggests that note taking can improve academic achievement, but current research has failed to report how low achievers might benefit from using note taking during math classes. The purpose of this study was to determine if teaching students structured note…

  9. Notes on the quantum tetrahedron

    CERN Document Server

    Coquereaux, Robert

    2002-01-01

    This is a set of notes describing several aspects of the space of paths on ADE Dynkin diagrams, with a particular attention paid to the graph E6. Many results originally due to A. Ocneanu are here described in a very elementary way (manipulation of square or rectangular matrices). We define the concept of essential matrices for a graph and describe their module properties with respect to right and left actions of fusion algebras. In the case of the graph E6, essential matrices build up a right module with respect to its fusion algebra but a left module with respect to the fusion algebra of A11. We present two original results: 1) We show how to recover the Ocneanu graph of quantum symmetries of the Dynkin diagram E6 from the natural multiplication defined in the tensor square of its fusion algebra (the tensor product should be taken over a particular subalgebra); this is the Cayley graph for the two generators of the twelve dimensional algebra (E6 \\otimes_A3 E6); here A3 and E6 refer to the commutative fusion...

  10. Integrator backstepping using contraction theory: a brief technological note

    DEFF Research Database (Denmark)

    Jouffroy, Jerome; Lottin, Jacques

    While the use of Lyapunov function candidates for integrator backstepping has been extensively studied in the literature, little research has been conducted regarding the applicability of the so-called incremental stability approaches. This note addresses the problem of the use of an incremental ...

  11. Note Passing and Gendered Discipline in Vietnamese Schools

    Science.gov (United States)

    Horton, Paul

    2015-01-01

    While researchers agree that note passing is predominantly an activity engaged in by girls, there has been relatively little consideration of why this is the case. In this article, I argue that gendered expectations about the appropriate characters of boys and girls in Vietnam are incorporated into the disciplinary framework of schools, and that…

  12. A note on contemporary psychometrics.

    Science.gov (United States)

    Vitoratou, Silia; Pickles, Andrew

    2017-12-01

    Psychometrics provide the mathematical underpinnings for psychological assessment. From the late 19th century, a plethora of methodological research achievements equipped researchers and clinicians with efficient tools whose practical value becomes more evident in the era of the internet and big data. Nowadays, powerful probabilistic models exist for most types of data and research questions. As the usability of the psychometric scales is better comprehended, there is an increased interest in applied research outcomes. Paradoxically, while the interest in applications for psychometric scales increases, publishing research on the development and/or evaluation of those scales per se, is not welcomed by many relevant journals. This special issue in psychometrics is therefore a great opportunity to briefly review the main ideas and methods used in psychometrics, and to discuss the challenges in contemporary applied psychometrics.

  13. Open Oncology Notes: A Qualitative Study of Oncology Patients' Experiences Reading Their Cancer Care Notes.

    Science.gov (United States)

    Kayastha, Neha; Pollak, Kathryn I; LeBlanc, Thomas W

    2018-04-01

    Electronic medical records increasingly allow patients access to clinician notes. Although most believe that open notes benefits patients, some suggest negative consequences. Little is known about the experiences of patients with cancer reading their medical notes; thus we aimed to describe this qualitatively. We interviewed 20 adults with metastatic or incurable cancer receiving cancer treatment. The semistructured qualitative interviews included four segments: assessing their overall experience reading notes, discussing how notes affected their cancer care experiences, reading a real note with the interviewer, and making suggestions for improvement. We used a constant comparison approach to analyze these qualitative data. We found four themes. Patients reported that notes resulted in the following: (1) increased comprehension; (2) ameliorated uncertainty, relieved anxiety, and facilitated control; (3) increased trust; and (4) for a subset of patients, increased anxiety. Patients described increased comprehension because notes refreshed their memory and clarified their understanding of visits. This helped mitigate the unfamiliarity of cancer, addressing uncertainty and relieving anxiety. Notes facilitated control, empowering patients to ask clinicians more questions. The transparency of notes also increased trust in clinicians. For a subset of patients, however, notes were emotionally difficult to read and raised concerns. Patients identified medical jargon and repetition in notes as areas for improvement. Most patients thought that reading notes improved their care experiences. A small subset of patients experienced increased distress. As reading notes becomes a routine part of the patient experience, physicians might want to elicit and address concerns that arise from notes, thereby further engaging patients in their care.

  14. Research

    African Journals Online (AJOL)

    A descriptive qualitative research design was used to determine whether participants ... simulation as a teaching method; a manikin offering effective learning; confidence ..... Tesch R. Qualitative Research: Analysis Types and Software Tools.

  15. Natural orifice translumenal endoscopic surgery (NOTES) for innovation in hepatobiliary and pancreatic surgery: preface.

    Science.gov (United States)

    Sugimoto, Maki

    2009-01-01

    Natural orifice translumenal endoscopic surgery (NOTES) has captured the interest of interventional endoscopists and may represent the next stage of evolution of minimally invasive surgery. It provides the potential for performance of incisionless operations. It is gaining momentum both in the animal laboratory and in human case reports. Developments in the field of NOTES have led to the formation of the Natural Orifice Surgery Consortium for Assessment and Research (NOSCAR) in 2006. In this special issue, the current trends in NOTES in the field of hepatobiliary and pancreatic surgery are featured, including NOTES cholecystectomy, hepatectomy splenectomy, pancreatic necrosectomy, and the future of NOTES. In this issue, we discuss the potential benefits of these procedures in hepatobiliary and pancreatic surgery. We have just started the evaluation process for this new technology. The concept of NOTES is becoming established and is enormously advantageous for the patient. Both the surgeon and gastroenterologist should contribute to developing NOTES in making use of their specialties.

  16. Research

    African Journals Online (AJOL)

    research process, as part of which students must find and appraise evidence from research.[5] This highlights that teaching research methodology is inclined towards equipping students ... Students believed that evidence-based practice was vital, yet their understanding of the concept was restricted when compared with the.

  17. Station Program Note Pull Automation

    Science.gov (United States)

    Delgado, Ivan

    2016-01-01

    Upon commencement of my internship, I was in charge of maintaining the CoFR (Certificate of Flight Readiness) Tool. The tool acquires data from existing Excel workbooks on NASA's and Boeing's databases to create a new spreadsheet listing out all the potential safety concerns for upcoming flights and software transitions. Since the application was written in Visual Basic, I had to learn a new programming language and prepare to handle any malfunctions within the program. Shortly afterwards, I was given the assignment to automate the Station Program Note (SPN) Pull process. I developed an application, in Python, that generated a GUI (Graphical User Interface) that will be used by the International Space Station Safety & Mission Assurance team here at Johnson Space Center. The application will allow its users to download online files with the click of a button, import SPN's based on three different pulls, instantly manipulate and filter spreadsheets, and compare the three sources to determine which active SPN's (Station Program Notes) must be reviewed for any upcoming flights, missions, and/or software transitions. Initially, to perform the NASA SPN pull (one of three), I had created the program to allow the user to login to a secure webpage that stores data, input specific parameters, and retrieve the desired SPN's based on their inputs. However, to avoid any conflicts with sustainment, I altered it so that the user may login and download the NASA file independently. After the user has downloaded the file with the click of a button, I defined the program to check for any outdated or pre-existing files, for successful downloads, to acquire the spreadsheet, convert it from a text file to a comma separated file and finally into an Excel spreadsheet to be filtered and later scrutinized for specific SPN numbers. Once this file has been automatically manipulated to provide only the SPN numbers that are desired, they are stored in a global variable, shown on the GUI, and

  18. NOTE TAKING PAIRS TO IMPROVE STUDENTS‟ SENTENCE BASED WRITING ACHIEVEMENT

    Directory of Open Access Journals (Sweden)

    Testiana Deni Wijayatiningsih

    2017-04-01

    Full Text Available Students had skill to actualize their imagination and interpret their knowledge through writing which could be combined with good writing structure. Moreover, their writing skill still had low motivation and had not reached the standard writing structure. Based on the background above, this research has purpose to know the influence Note Taking Pairs in improving students‘sentence based writing achievement. The subject of this research was the second semester of English Department in Muhammadiyah University of Semarang. It also used statistic non parametric method to analyze the students‘ writing achievement. The result of this research showed that Note Taking Pairs strategy could improve students‘sentence based writing achievement. Hopefully this research is recommended into learning process to improve students‘writing skill especially in sentence-based writing subject.

  19. Book Reviews/ Notes de lecture

    African Journals Online (AJOL)

    sulaiman.adebowale

    2006-08-29

    Aug 29, 2006 ... 189–194. © Council for the Development of Social Science Research in Africa, 2006 ... Globalization, for instance, has undermined the legitimacy and power .... The third chapter by Anna Madoeff examines the role of Moulids.

  20. Employers' views on the fit note.

    Science.gov (United States)

    Kotze, E

    2014-12-01

    The fit note replaced the sick note in 2010. The statement of fitness for work (fit note) is expected to benefit the British economy by helping more people stay in work and prevent long-term sickness absence. Understanding and responding to employers' views on the fit note is key, in order for this goal to be achieved. To explore employers' views on the fit note. A qualitative study was undertaken and face-to-face interviews were conducted with participants representing employers from a variety of industries. There were 21 participants who were mainly human resources officers and line managers. Employers welcomed the introduction of the fit note and felt that it was an improvement on the sick note. The majority of employers felt the fit note had the potential to promote an earlier return to work, if used properly. The main problems reported were the completion of the fit notes and quality of advice received from general practitioners. Employers felt that the most helpful advice came from fit notes with information on the functional effects of the medical condition. Some employers found return to work decisions problematic. The fit note has the potential to promote an earlier return to work. In order for the fit note to achieve its aim, further understanding of the difficulties employers are having when making return to work decisions is important, in order to develop guidance to enable them to provide the practical support employees need to return to work sooner. © The Author 2014. Published by Oxford University Press on behalf of the Society of Occupational Medicine. All rights reserved. For Permissions, please email: journals.permissions@oup.com.