Micropropagation of Origanum acutidens (HAND.-MAZZ.) IETSWAART using stem node explants.

Science.gov (United States)

Yildirim, Mehmet Ugur

2013-01-01

Origanum acutidens (HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0.6, 1.2, 1.8, and 2.4 mg/L BAP with 0.2 mg/L NAA. Visible effects of culture media on shoot proliferation were recorded. Shoot regeneration rate was maximum on MS medium containing 1.80 mg/L BAP-0.2 mg/L NAA. The micropropagated shoots were rooted on MS medium containing 0.2 mg/L NAA. All microrooted plantlets survived during acclimatisation on peat moss. It was concluded that O. acutidens can be successfully micropropagated under in vitro conditions.

Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP).

Science.gov (United States)

Peraza-Echeverria, S; Herrera-Valencia, V A.; Kay, A -J.

2001-07-01

The extent of DNA methylation polymorphisms was evaluated in micropropagated banana (Musa AAA cv. 'Grand Naine') derived from either the vegetative apex of the sucker or the floral apex of the male inflorescence using the methylation-sensitive amplification polymorphism (MSAP) technique. In all, 465 fragments, each representing a recognition site cleaved by either or both of the isoschizomers were amplified using eight combinations of primers. A total of 107 sites (23%) were found to be methylated at cytosine in the genome of micropropagated banana plants. In plants micropropagated from the male inflorescence explant 14 (3%) DNA methylation events were polymorphic, while plants micropropagated from the sucker explant produced 8 (1.7%) polymorphisms. No DNA methylation polymorphisms were detected in conventionally propagated banana plants. These results demonstrated the usefulness of MSAP to detect DNA methylation events in micropropagated banana plants and indicate that DNA methylation polymorphisms are associated with micropropagation.

Endophytes in commercial micropropagation - friend or foe?

Directory of Open Access Journals (Sweden)

Rödel, Philipp

2016-07-01

Full Text Available Medicinal and aromatic plants are superorganisms like all plant species- naturally colonized by bacteria, fungi and protists. Micropropagated plants are facing different challenges under in vitro and ex vitro conditions: Mixotrophic growth under low light conditions on artificial nutrient media, poor gas exchange in small vessels, abiotic stress, bad rooting, transplanting stress, low survival rate during acclimatization in greenhouse. The use of endophytes in micropropagation can improve plant growth, yield, and health and induce tolerance to abiotic and biotic stress. A tool for the use of competent endophytes in micropropagation under in vitro and ex vitro conditions is “biotization” of plantlets with useful bacterial and fungal inocula. Fungal inocula which are used commercially are e.g. arbuscular mycorrhizal fungi in form of spores and extraradical mycelium on different carrier materials like expanded clay, vermiculite, sand or peat. Furthermore representatives of the root fungal genus Trichoderma are applied as spores formulated in powder. Plantgrowth promoting rhizobacteria of the important genera Bacillus, Pseudomonas, Azospirillum and Azotobacter in form of lyophilised endospores/bacterial cells in powder or liquid formulation are also available on the market.

Micropropagation of rose cultivar 'Pareo'

African Journals Online (AJOL)

Jane

2011-08-22

Aug 22, 2011 ... Serik Mukhambetzhanov*, Svetlana Nam, Valentina Mursalieva and Izbasar Rakhimbaev. Laboratory of Clonal Propagation, Institute of Plant Biology and Biotechnology, 050040 Almaty, Kazakhstan. Accepted 1 July, 2011. A protocol was developed for micropropagation of rose cv. 'Pareo'. Nodal segments ...

Acclimatization of Phalaenopsis and Cattleya obtained by micropropagation

OpenAIRE

Lucía Primitiva Díaz; Jorge Julio Namur; Sebastián Agustín Bollati; Osvaldo Ernesto Antonio Arce

2010-01-01

Título en español: Aclimatización de Phalaenopsis y Cattleya obtenidas por micropropagación ABSTRACT The quality of micropropagated plants relies on the acclimatization stage. This research intends to develop an efficient protocol to obtain the acclimatization of Phalaenopsis and Cattleya. Plants of Phalaenopsis obtained from protocorms were selected. They came from flowering stalks grown at modified Murashige and Skoog (MS) (1962) medium and classified by growth ranks and put into mo...

Micropropagation of Eucalyptus grandis and nitens using tissue culture techniques

Energy Technology Data Exchange (ETDEWEB)

Furze, M.J.; Creswell, C.F.

1985-01-01

Experiments with nodal explants of E. grandis and E. nitens seedlings and E. grandis coppice shoots showed that a large number of plants can be produced from a single explant using micropropagation. The percentage of micropropagated shoots that formed roots was about 90% for E. grandis and 80% for E. nitens. For both species, about 90% of the rooted shoots survived after hardening off. 9 references.

Micropropagation of Plantago camtschatica Link

Directory of Open Access Journals (Sweden)

Emilia Andrzejewska-Golec

2011-01-01

Full Text Available The Far East medicinal plant - Plantago camtschatica was propagated in vitro from tips of shoots (obtained in vitro and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots, first leaves. To our knowledge there is no information in literature about in vitro culture of this plantain. MS basal medium, supplemented with 0.6 pM IAA in combination with various cytokinins (BA, KIN, ZEA, was used. After 6 weeks of culture, micropropagation rate (MR - mean number of buds and shoots per explant - was calculated. Our study proved that P. camtschatica species was amenable to propagation in vitro from different kinds of explants. However, multiplication by adventitious shoot regeneration from hypocotyl explants was found to be the most suitable method for the propagation of this plant. Adventitious shoots could root without stimulation what allows to omit the stage of rooting. The plants obtained as a result of micropropagation were not phenotypically changed.

RESEARCH NOTE

Indian Academy of Sciences (India)

Navya

RESEARCH NOTE. CDKN2A and MC1R ... Department of Pharmacy and Department of Nursing, School of Health Sciences, Frederick. University, Nicosia ..... Appears with highest frequency in African, Asian-Indian, and Papua. New Guinean ...

Dendrobium micropropagation: a review.

Science.gov (United States)

da Silva, Jaime A Teixeira; Cardoso, Jean Carlos; Dobránszki, Judit; Zeng, Songjun

2015-05-01

Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review. Not only will this expand the use for mass propagation, but will also allow for the conservation of important germplasm. Information on the in vitro responses of Dendrobium for developing efficient protocols for breeding techniques based on tissue culture, such as polyploidization, somatic hybridization, isolation of mutants and somaclonal variants and for synthetic seed and bioreactor technology, or for genetic transformation, is discussed in this review. This is the first such review on this genus and represents half a decade of literature dedicated to Dendrobium micropropagation.

A REVIEW: MICROPROPAGATION OF PHALAENOPSIS sp FROM LEAF AND FLOWER STALK EXPLANTS

Directory of Open Access Journals (Sweden)

Meutia Zahara

2017-09-01

• Semiarti, E.; Indrianto, A.; Purwanto, A. Agrobacterium-Mediated transformation of Indonesian orchids for  micropropagation, genetic transformation, Prof. MarÃa Alvarez (Ed., ISBN: 978-953-307-364-4, InTech, 2011. Available from: http://www.intechopen.com/books/ genetic-transformation/agrobacterium-mediated-transformation-ofindonesian-orchids-for-micropropagation.

Micropropagation of Asparagus by in vitro shoot culture.

Science.gov (United States)

Stajner, Nataša

2013-01-01

Asparagus officinalis is most extensively studied species within the genus Asparagus, which is well known as garden asparagus. This species is dioecious with unisexual flowers, which means that generative propagation gives roughly equal number of male and female plants. Male plants are high yielders and preferred commercially over female plants. Tissue culture techniques could efficiently promote vegetative propagation of male plants and pave the way for efficient plant breeding.This chapter describes an efficient micropropagation protocol for developing rapid growing in vitro Asparagus shoot cultures. The source of explants, inoculation, and shoot proliferation, followed by shoot propagation, rooting, and acclimatization is described. The optimal medium for Asparagus micropropagation described in this chapter is composed of MS macro- and microelements and a combination of auxins and cytokinins. Plant growth regulators NAA, kinetin, and BA were used in various concentrations. Three different media representing the whole micropropagation protocol of Asparagus are described; medium for shoot initiation, medium for shoot multiplication, and medium for root formation. By in vitro propagation of Asparagus, root initiation is difficult, but can be promoted by adding growth retardant ancymidol which also greatly promotes shoot development and suppresses callus formation.

Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench

Science.gov (United States)

2012-08-01

1105 Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench Luiz H...fungal community and micropropagated clones of E. purpurea was re-established after acclimatization to soil and the endophytic fungi produced compounds...Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant Echinacea purpurea (L.) Moench 5a. CONTRACT

Micropropagation of Pithecellobium dulce (Roxb.) Benth-a multipurpose leguminous tree and assessment of genetic fidelity of micropropagated plants using molecular markers.

Science.gov (United States)

Goyal, Pooja; Kachhwaha, Sumita; Kothari, S L

2012-04-01

An efficient and reproducible protocol has been developed for in vitro propagation of Pithecellobium dulce (Roxb.) Benth (a multipurpose leguminous tree) from field grown nodal segments (axillary bud). Shoot bud induction occurred from nodal explants of 15-years-old tree on Murashige and Skoog (MS) basal medium supplemented with 4.4 μM 6-benzyladenine (BA) and multiplication was achieved on MS medium supplemented with 4.4 μM BA + 0.73 μM phenylacetic acid (PAA) i.e. up to 7 shoot buds in the period of 5-6 weeks. Addition of adenine sulphate (AdS) to this medium further enhanced the number of shoot buds up to 10. Proliferating shoot cultures were established by repeatedly subculturing primary culture on fresh medium (MS + 4.4 μM BA + 0.73 μM PAA) after every 25 days. In vitro rooting was achieved on MS medium supplemented with 2.46 μM Indole-3-butyric acid (IBA) + 41.63 μM activated charcoal (AC). The micropropagated shoots with well developed roots were acclimatized in green house in pots containing sand, soil and manure (1:1:1). Genetic stability of micropropagated clones was evaluated using Random amplified polymorphic DNA (RAPD) and Inter simple sequence repeat (ISSR) markers. The amplification products were monomorphic in micropropagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic uniformity of micropropagated plants. This is the first report of an efficient protocol for regeneration of P. dulce through organogenesis, which can be used for further genetic transformation and pharmaceutical purposes.

Micropropagation of Plectranthus edulis (Vatke) Agnew from ...

African Journals Online (AJOL)

SAM

2014-09-03

Sep 3, 2014 ... Key words: In vitro propagation, meristem culture, micropropagation, Plectranthus edulis (Vatke) Agnew, shoots multiplication. ... P. edulis showed the occurrence of diseases (Taye,. 2008). ..... Asian J. Plant Sci. 2:616-622.

Micropropagation, Acclimatization, and Greenhouse Culture of Veratrum californicum.

Science.gov (United States)

White, Sarah A; Adelberg, Jeffrey; Naylor-Adelberg, Jacqueline; Mann, David A; Song, Ju Yeon; Sun, Youping

2016-01-01

Micropropagation and production of Veratrum californicum is most successful when using a premixed Murishage and Skoog basal medium with vitamins and a 5-week subculture cycle at 16 °C for multiplication. These culture conditions provide the best percent survival after acclimatization in the greenhouse. However, clone response to temperature and light quality within culture conditions varies. Micropropagated plants have mass and morphology similar to 2- or 3-year-old seedlings. Acclimatized plantlets can then be grown in the greenhouse using sub-irrigation (ebb and flood) to maintain substrate volumetric water content > 44 %. Growth cycle in the greenhouse must be about 100 days, followed by dormancy for 5 months at 5 °C.

Visual object recognition for automatic micropropagation of plants

Science.gov (United States)

Brendel, Thorsten; Schwanke, Joerg; Jensch, Peter F.

1994-11-01

Micropropagation of plants is done by cutting juvenile plants and placing them into special container-boxes with nutrient-solution where the pieces can grow up and be cut again several times. To produce high amounts of biomass it is necessary to do plant micropropagation by a robotic system. In this paper we describe parts of the vision system that recognizes plants and their particular cutting points. Therefore, it is necessary to extract elements of the plants and relations between these elements (for example root, stem, leaf). Different species vary in their morphological appearance, variation is also immanent in plants of the same species. Therefore, we introduce several morphological classes of plants from that we expect same recognition methods.

Analyzing clonal fidelity of micropropagated Psidium guajava L. plants using simple sequence repeat markers

Science.gov (United States)

Micropropagation of Psidium guajava L. (guava) is a viable alternative to currently adopted techniques for large-scale plant propagation of commercial cultivars. Assessment of clonal fidelity in micropropagated plants is the first step towards ensuring genetic uniformity in mass production of planti...

Detection of somaclonal variation in micropropagated Hibiscus ...

African Journals Online (AJOL)

User

Paper Accepted on 26 July 2010. Abstract. The main ... micropropagated propagules of ornamental pineapple (Santos et al., 2008) and in cucumber ... fleshy calyces are used for wine making, juice, jam, jelly, syrup, gelatin, pudding, ice cream ...

Micropropagation and genetic transformation of Tylophora indica (Burm. f.) Merr.: a review.

Science.gov (United States)

Teixeira da Silva, Jaime A; Jha, Sumita

2016-11-01

This review provides an in-depth and comprehensive overview of the in vitro culture of Tylophora species, which have medicinal properties. Tylophora indica (Burm. f.) Merr. is a climbing perennial vine with medicinal properties. The tissue culture and genetic transformation of T. indica, which has been extensively studied, is reviewed. Micropropagation using nodal explants has been reported in 25 % of all publications. Leaf explants from field-grown plants has been the explant of choice of independent research groups, which reported direct and callus-mediated organogenesis as well as callus-mediated somatic embryogenesis. Protoplast-mediated regeneration and callus-mediated shoot organogenesis has also been reported from stem explants, and to a lesser degree from root explants of micropropagated plants in vitro. Recent studies that used HPLC confirmed the potential of micropropagated plants to synthesize the major T. indica alkaloid tylophorine prior to and after transfer to field conditions. The genetic integrity of callus-regenerated plants was confirmed by RAPD in a few reports. Tissue culture is an essential base for genetic transformation studies. Hairy roots and transgenic T. indica plants have been shown to accumulate tylophorine suggesting that in vitro biology and transgenic methods are viable ways of clonally producing valuable germplasm and mass producing compounds of commercial value. Further studies that investigate the factors affecting the biosynthesis of Tylophora alkaloids and other secondary metabolites need to be conducted using non-transformed as well as transformed cell and organ cultures.

Efficient micropropagation and chlorocholine chloride induced stevioside production of Stevia rebaudiana Bertoni.

Science.gov (United States)

Dey, Avishek; Kundu, Sayanti; Bandyopadhyay, Abhijit; Bhattacharjee, Aloke

2013-01-01

A promising method of micropropagation of Stevia rebaudiana Bertoni has been developed with an aim to increase the biomass, survivability of the plantlets and stevioside production, using chlorocholine chloride (CCC). Microshoots transferred to the MS medium containing different combinations CCC and IBA were found to be most effective in terms of growth pattern, hardening ability of the plantlets and stevioside content, compared to MS medium containing either IBA or CCC. Among other combinations tested, MS medium supplemented with 3 mg/l CCC and 3 mg/l IBA was found most effective in inducing significant changes like reduced shoot length, increased number of roots, higher leaf size, increased biomass and chlorophyll retaining capacity, higher survival percentage and most importantly the elevated stevioside content. Collectively, the major observations of this research indicate that application of CCC in micropropagation of S. rebaudiana Bertoni is a promising approach and has commercial prospects. Copyright © 2012 Académie des sciences. Published by Elsevier SAS. All rights reserved.

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from ...

African Journals Online (AJOL)

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants. Evelyn Duarte, Pedro Sansberro, Claudia Luna. Abstract. Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with ...

Comparative study of potato cultivation through micropropagation ...

African Journals Online (AJOL)

sonu

Comparative study of potato cultivation through micropropagation and conventional farming methods .... and Murate potash were used as fertilizer source of nitrogen, phosphorus and potassium, respectively (Table 1) ..... through Tissue culture- Application and Feasibility. U.S.D.A.,. Beltsville. Agric. Res. Sci. Educ. Admin.

Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles.

Science.gov (United States)

Puente-Garza, César A; Gutiérrez-Mora, Antonia; García-Lara, Silverio

2015-01-01

Maguey, Agave salmiana, is an important plant for the "pulque" beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX) activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild-type (WT) plants, in in vitro (IN) plants, and ex vitro acclimated plants (EN). The results showed that IN plants have a 50% lower soluble sugar content compared to WT, and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN) and regenerated (EN) plants compared to WT. The total saponin content in IN, and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = -0.927; p = 0.003) was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to WT plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants.

Micropropagation of Agave salmiana: Means to production of antioxidant and bioactive principles.

Directory of Open Access Journals (Sweden)

César Armando Puente-Garza

2015-11-01

Full Text Available Maguey, Agave salmiana, is an important plant for the pulque beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild type (WT plants, in in vitro (IN plants, and ex vitro acclimated plants (EN. The results showed that IN plants have a 50% lower soluble sugar content compared to WT and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN and regenerated (EN plants compared to WT. The total saponin content in IN and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = -0.927; p = 0.003 was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to wild plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants.

Note Taking on Trial: A Legal Application of Note-Taking Research

Science.gov (United States)

Kiewra, Kenneth A.

2016-01-01

This article is about note taking, but it is not an exhaustive review of note-taking literature. Instead, it portrays the application of note-taking research to an unusual and important area of practice--the law. I was hired to serve as an expert witness on note taking in a legal case that hinged, in part, on the completeness and accuracy of…

Successful micropropagation of the cadmium hyperaccumulator Viola baoshanensis (Violaceae).

Science.gov (United States)

Li, Jin-Tian; Deng, Dong-Mei; Peng, Guang-Tian; Deng, Jin-Chuan; Zhang, Jun; Liao, Bin

2010-01-01

Viola baoshanensis is one of the most rare cadmium (Cd) hyperaccumulators, however, it is hard to propagate. Micropropagation has been applied to solve the problems with propagation of a few heavy metal hyperaccumulators. Therefore there is a high likelihood that micropropagation may offer a suitable method for large-scale propagation of V. baoshanensis To test this hypothesis, three types of explants were used for shoot regeneration and various combinations of four plant growth regulators were used to improve shoot regeneration efficiency from leaflet of V. baoshanensis. Best shoot regeneration efficiency was obtained by incubating leaflet in a 1/2 MS medium supplemented with 2.5 oM BA + 2.5 microM IBA, therein shoot regeneration rate was 70.9% and the number of shoots formation per explant was 22.4. Rooting was achieved from almost all regenerated shoot growing on 1/2 MS medium without plant growth regulator. Micropropagated seedlings were acclimatized under greenhouse conditions and 95% of them survived and showed no visible morphological variation compared to their donor plant. Furthermore, there were no significant differences between regenerated and seed-germinated V. baoshanensis in Cd tolerance and accumulation. These results suggested that an efficient and rapid micropropogation system was successfully developed for V. baoshanensis.

Detection of somaclonal variation in micropropagated Hibiscus ...

African Journals Online (AJOL)

The main objective of micropropagation is to produce clones i.e. plants which are phenotypically and genetically identical to the mother plants. The culture of organized meristems usually guarantees the production of true-to-type plants but variations in the progenies have been widely reported. Hibiscus sabdariffa L. plants ...

Micropropagation of tulip: production of virus-free stock plants.

Science.gov (United States)

Podwyszyńska, Małgorzata; Sochacki, Dariusz

2010-01-01

We describe here a new tulip micropropagation method based on the cyclic shoot multiplication in presence of the thidiazuron (TDZ), which enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. In our novel protocol, cyclic shoot multiplication can be performed for 2-3 years by using TDZ instead of other cytokinins, as 6-benzylaminopurine (BAP) and N(6)-(-isopentyl)adenine (2iP). It makes possible to produce 500-2,000 microbulbs from one healthy plant. There are six main stages of tulip micropropagation. Stage 0 is the selection of true-to-type and virus-free plants, confirmed by ELISA. Fragments of flower stems isolated from bulbs are used as initial explants. Shoot multiplication is based on the regeneration of adventitious shoots, which are sub-cultured every 8 weeks. In the Stage 3, the specially prepared shoots are induced by low temperature treatment to form bulbs which finally develop on a sucrose-rich medium at 20 degrees C. Bulbs are then dried for 6 weeks and rooted in vivo. The number of multiplication subcultures should be limited to 5-10 cycles in order to lower the risk of mutation. Virus indexing should be repeated 3-4 times, at the initial stage and then during shoot multiplication. Genetic stability of micropropagated shoots can be confirmed using molecular markers.

Effect of arbuscular mycorrhiza on the growth and development of micropropagated Annona cherimola plants

Directory of Open Access Journals (Sweden)

Concepcion Azcón-Aguilar

1994-05-01

Full Text Available Annona cherimola Mill., cherimoya, is a tropical plantation crop of interest in fruit culture. Micropropagation techniques have been developed due to the need to increase productivity through clonal selection. Because of the mycorrhizal dependence exhibited by this crop for optimal growth and the recognized role of mycorrhiza establishment for the survival and development of most of the plants produced in vitro, the effect of mycorrhiza inoculation on the development of micropropagated plants of Annona cherimola was investigated. Mycorrhizal inoculation was assayed at two different stages of the micropropagation process: (i immediately after the in vitro phase, before starting the acclimatization period, and (ii after the acclimatization phase, before starting the post-acclimatization period under greenhouse conditions. Plantlet survival was about 50 % after the acclimatization period. Plant growth and development profited remarkably from mycorrhiza establishment. Most of the arbuscular mycorrhizal fungi (AMF assayed greatly increased shoot and root biomass and leaf area. Micropropagated Annona plants seem to be more dependent on mycorrhiza formation for optimal growth than plants derived from seeds. The greatest effects of AMF on plant growth were observed when they were introduced after the acclimatization period.

Micropropagation of pear (Pyrus sp.).

Science.gov (United States)

Reed, Barbara M; Denoma, Jeanine; Wada, Sugae; Postman, Joseph

2013-01-01

Elements of micropropagation include establishment of shoot tip cultures, proliferation, rooting, and acclimatization of the resulting plantlets. The wide genetic variation in Pyrus makes micropropagation challenging for many genotypes. Initiation of shoots is most successful from forced dormant shoots or from scions grafted onto seedling rootstocks to impose juvenility. Clean shoots are recovered after testing for contaminants at the initiation stage on ½ strength Murashige and Skoog 1962 medium (MS), at pH 6.9 for 1 week or by streaking on nutrient agar. Although pear species and cultivars are cultured on several well-known media, MS is the most commonly used. Our studies showed that multiplication and growth of shoots are best on Pear Medium with higher concentrations of calcium chloride, potassium phosphate, and magnesium sulfate than MS medium and 4.4 μM N(6) benzyladenine. Pear shoots are often recalcitrant to rooting; however, a 5 s dip in 10 mM indole-3-butyric acid or naphthalene acetic acid before planting on basal medium without plant growth regulators is effective for many genotypes. Pear shoots store well at 1-4°C, and can hold for as long as 4 years without reculture. Cryopreservation protocols are available for long-term storage of pear shoot tips. Acclimation of in vitro-rooted or micrografted shoots in a mist bed follows standard procedures.

Associated microbial contaminants in in-vitro micropropagation of ...

African Journals Online (AJOL)

Studies were carried out to determine the microbial contaminants associated with in-vitro micropropagation of Ipomea batatas (sweet potato). The contaminants were found to be mostly fungal organisms, Aspergillus Spp (62%), Penicillum Spp. (31%), Fusarium Spp. (5%) and Alternaria Spp. (2%). Bacterial contamination ...

Micropropagation, genetic engineering, and molecular biology of Populus

Science.gov (United States)

N. B. Klopfenstein; Y. W. Chun; M. -S. Kim; M. A. Ahuja; M. C. Dillon; R. C. Carman; L. G. Eskew

1997-01-01

Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3) molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease,...

Rapid micropropagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl.

African Journals Online (AJOL)

Rapid micropropagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl. (a valuable medicinal plant) from shoot bud explants. NA Yusuf, MMS Annuar, N Khalid. Abstract. A successful protocol was developed for mass propagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl., an important medicinal plant. Numerous shoots ...

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a ...

African Journals Online (AJOL)

The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semisolid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA®), comparing results with those obtained using a ...

Mentha piperita micropropagation and volatil constituents evaluation

Directory of Open Access Journals (Sweden)

Jorge Ignacio Zapata

1999-01-01

Full Text Available The use of in vitro plant tissue culture techniques for the propagation of aromatic and medicinal species, could play very promising role for the economy of small farmers, due to the high potential of industrialization of their essential oils. In this work is shown how the tissue culture technique has been used for the propagation of Mentha piperita, as its essential oil is widely used in the flavor and fragrance industry. Mints from different sources were studied, and evaluation of the yield and quality of the essential oil was carry out, before and after the micropropagation by plant tissue culture. Several basal mediums with different proportion of plant growth regulators and vitamins were tested in order to maximize the propagation results. The constituents of the essential oil were quantified by Gas Chromatography. Lin & Staba (1962 basal medium, supplemented with BAP, and some vitamins, was the best for mints species micropropagation. The yield and quality of essential oil produced by plant tissue culture and its mother plant were very similar.

Thin Cell Layer technology in ornamental plant micropropagation ...

African Journals Online (AJOL)

Thin cell layer (TCL) technology originated almost 30 years ago with the controlled development of flowers, roots, shoots and somatic embryos on tobacco pedicel longitudinal TCLs. Since then TCLs have been successfully used in the micropropagation of many ornamental plant species whose previous in vitro ...

Yellow-cedar in vitro clonal production and evaluation of propagules for reforestation. FRDA research memo No. 211

Energy Technology Data Exchange (ETDEWEB)

1992-01-01

Technical note describing a project that was part of an ongoing effort to develop micropropagation techniques on an operation scale and to investigate their possible use in producing genetically improved stock for planting. The project developed a commercially viable process that uses organogenic micropropagation to produce yellow-cedar stock for operational reforestation; evaluated the techniques for developing genetically improved clones; and established demonstration plots of mircopropagules.

Micropropagation of some Malaysian banana and plantain (Musa sp ...

African Journals Online (AJOL)

USER

2010-04-19

Apr 19, 2010 ... As one of the origins of bananas, Malaysia has a great variety of them, that is, ... reduces contamination rate during micropropagation as compared to soil ..... 42. Faostat (2005). Food and Agricultural Organization of the United.

Bite-size research: BMC Research Notes goes back to its roots.

Science.gov (United States)

Krüger, Dirk; Marshall, Diana M

2017-02-14

Since it first launched in 2008, BMC Research Notes has been a place where researchers can publish short notes and observations, research outputs which are useful for the community but which can end up hidden in the lab notebook or as a footnote in a dataset. In order to re-affirm the importance of publishing these kinds of outputs, the journal is renewing its focus on publishing note articles as well as other potentially dark data such as short null results. Publishing these articles is useful for many researchers, therefore we are also expanding the scope to all scientific and clinical disciplines including health sciences, life sciences, physical sciences, mathematics and all engineering disciplines. With this refocusing of BMC Research Notes back to its original vision, BioMed Central is offering a home for short communications to make dark data and single observations widely available to the global research community.

Genetic characterization, micropropagation, and potential use for arsenic phytoremediation of Dittrichia viscosa (L.) Greuter.

Science.gov (United States)

Guarino, Francesco; Conte, Barbara; Improta, Giovanni; Sciarrillo, Rosaria; Castiglione, Stefano; Cicatelli, Angela; Guarino, Carmine

2018-02-01

In the last decade, many scientists have focused their attention on the search for new plant species that can offer improved capacities to reclaim polluted soils and waters via phytoremediation. In this study, seed batches from three natural populations of Dittrichia viscosa, harvested in rural, urban, and industrial areas of central and southern Italy, were used to: (i) evaluate the genetic and morphological diversity of the populations; (ii) develop an efficient protocol for in-vitro propagation from seedling microcuttings; (iii) achieve optimal acclimatization of micropropagated plants to greenhouse conditions; (iv) test the response to arsenic (As) soil contamination of micropropagated plants. The genetic biodiversity study, based on Random Amplification of Polymorphic DNA (RAPD), as well as the morphometric analysis of 20 seedlings from each population revealed some degree of differentiation among populations. Based on these data, the most biodiverse plants from the three populations (10 lines each) were clonally multiplied by micropropagation using microcuttings of in-vitro grown seedlings. Three culture media were tested and Mureshige and Skoog medium was chosen for both seedling growth and micropropagation. The micropropagated plants responded well to greenhouse conditions and over 95% survived the acclimatization phase. Four clones were tested for their capacity to grow on soil spiked with NaAsO 2 and to absorb and accumulate the metalloid. All clones tolerated up to 1.0mg As. At the end of the trial (five weeks), As was detectable only in leaves of As-treated plants and concentration varied significantly among clones. The amount of As present in plants (leaves) corresponded to ca. 0.10-1.7% of the amount supplied. However, As was no longer detectable in soil suggesting that the metalloid was taken up, translocated and probably phytovolatilized. Copyright © 2017 Elsevier Inc. All rights reserved.

Micropropagation of eucalyptus saligna sm. from cotyledonary nodes

International Nuclear Information System (INIS)

Silva, A.L.L.D.; Brondani, G.E.; Horbach, A.

2015-01-01

Eucalyptus saligna is an important woody plant used to lumber and cellulose. The aim of this research was to establish a protocol for micropropagation of this species from cotyledonary nodes. Plantlets with 16 days old were used as a donor explants. The induction of cotyledonary nodes consisted of two parts: a dark culture followed by a light culture. Basal medium was MS added with 30g.L-1 sucrose, 10% coconut water and solidified with 7g.L-1 agar. For the dark culture the media were supplemented with 3.6 micro M NAA (Naftalenoacetic acid) and 4.4 micro M BAP (6-Benzylaminopurine) and for the light culture the media were supplemented with 2.7 micro M NAA and 1.1 micro M BAP. The period for dark and light culture was 20 days. Shoots were multiplied on MS medium, 30 g.L-1 sucrose supplemented with 1.1 micro M BAP. Shoots were elongated on MS medium free of plant growth regulators. Shoots were rooting on half-strength MS salts. Acclimatization was performed in a hydroponics floating system. Moreover, the shoot multiplication in liquid medium with different CaCl/sub 2/ levels was carried out under agitation. Organogenesis of cotyledonary nodes was characterized by simultaneous occurrence of shoot and callus. Shoots presented hyperhydricity under liquid medium, however, the CaCl/sub 2/ reduces the hyperhydricity in liquid medium; nevertheless, it had been not effective in eliminating hyperhydricity due to toxicity of chlorine. The hydroponics acclimatization results in 90% plant survival. An efficient protocol for micropropagation of E. saligna was suitable established and can be used for clonal propagation or genetic transformation. (author)

Evaluation of a New Temporary Immersion Bioreactor System for Micropropagation of Cultivars of Eucalyptus, Birch and Fir

Directory of Open Access Journals (Sweden)

Edward Businge

2017-06-01

Full Text Available The use of liquid instead of solid culture medium for the micropropagation of plants offers advantages such as better access to medium components and scalability through possible automation of the processes. The objective of this work was to compare a new temporary immersion bioreactor (TIB to solid medium culture for the micropropagation of a selection of tree species micropropagated for commercial use: Nordmann fir (Abies nordmanniana (Steven Spach, Eucalyptus (E. grandis x E. urophylla, Downy birch (Betula pubescens Ehrh, and Curly birch (Betula pendula var. carelica. Cultivation of explants in the TIB resulted in a significant increase of multiplication rate and fresh weight of Eucalyptus and B. pendula, but not Betula pubescens. In addition, the fresh weight of embryogenic tissue and the maturation frequency of somatic embryos increased significantly when an embryogenic cell line of A. nordmanniana was cultivated in the TIB compared to solid culture medium. These results demonstrate the potential for scaling up and automating micropropagation by shoot multiplication and somatic embryogenesis in commercial tree species using a temporary immersion bioreactor.

A novel approach for rapid micropropagation of maspine pineapple ...

African Journals Online (AJOL)

A novel approach for rapid micropropagation of maspine pineapple ( Ananas comosus L.) shoots using liquid shake culture system. ... Maspine (Ananas comosus L.) is currently the most preferred pineapple variety in Malaysia due to its pleasant aroma and applicability in caning. Large quantities of plant materials are ...

Micropropagation of Wild Service Tree (Sorbus torminalis [L.] Crantz): The Regulative Role of Different Aromatic Cytokinins During Organogenesis

Czech Academy of Sciences Publication Activity Database

Malá, J.; Máchová, P.; Cvrčková, H.; Karady, Michal; Novák, Ondřej; Mikulík, Jaromír; Hauserová, Eva; Greplová, Jarmila; Strnad, Miroslav; Doležal, Karel

2009-01-01

Roč. 28, č. 4 (2009), s. 341-348 ISSN 0721-7595 R&D Projects: GA ČR GA206/07/0570; GA MŠk 1M06030 Institutional research plan: CEZ:AV0Z50380511 Keywords : Wild service tree * Micropropagation * Rooting Subject RIV: EF - Botanics Impact factor: 2.438, year: 2009

Stalin and Marxism: a research note

NARCIS (Netherlands)

van Ree, E.

1997-01-01

This article concerns the research done by the author in Stalin‘s private library. The notes made in the works of Marx, Engels and Lenin suggest that until the end of his life Stalin felt himself in general agreement with these "classics." The choice of books and the notes support the thesis that,

A novel method for coral explant culture and micropropagation.

Science.gov (United States)

Vizel, Maya; Loya, Yossi; Downs, Craig A; Kramarsky-Winter, Esti

2011-06-01

We describe here a method for the micropropagation of coral that creates progeny from tissue explants derived from a single polyp or colonial corals. Coral tissue explants of various sizes (0.5-2.5 mm in diameter) were manually microdissected from the solitary coral Fungia granulosa. Explants could be maintained in an undeveloped state or induced to develop into polyps by manipulating environmental parameters such as light and temperature regimes, as well as substrate type. Fully developed polyps were able to be maintained for a long-term in a closed sea water system. Further, we demonstrate that mature explants are also amenable to this technique with the micropropagation of second-generation explants and their development into mature polyps. We thereby experimentally have established coral clonal lines that maintain their ability to differentiate without the need for chemical induction or genetic manipulation. The versatility of this method is also demonstrated through its application to two other coral species, the colonial corals Oculina patigonica and Favia favus.

Rapid micro-propagation of Aloe vera L. via shoot multiplication

African Journals Online (AJOL)

STORAGESEVER

2008-06-17

. Cloning protocol of Aloe vera as a study-case for tailor- made biotechnology to small farmers. J. Technol. Manag. Innov. 1: 76-79. Debiasi C, Silva CG, Pescador R (2007). Micropropagation of Aloe vera. L., Rev. Bras. PIant.

Microinitiation, micropropagation and damage

Energy Technology Data Exchange (ETDEWEB)

Savalle, S.; Cailletaud, G. (ONERA, 75 - Paris (France))

It is now well known that, on the microscopic scale, the fatigue failure process can be partitioned in two main stages: microinitiation and micropropagation. We show here the advantage in introducing these two successive steps in life prediction models for high temperature fatigue and creep. In a first part, we show that there is a non-interaction field between these two phenomena. Then, an extensive application is made using data on the Nickel-base alloy IN100: for some tests, creep and fatigue interact simultaneously, and for some others, they act consecutively. Finally, the same concepts are applied to the case of a loading corresponding to the superposition of vibrations and a large mean stress.

Micropropagation of Rubus spp.

Directory of Open Access Journals (Sweden)

Åke Finne

1986-09-01

Full Text Available Rapid micropropagation of ‘Black Satin’, ‘Thornless Evergreen’ and ‘Bedford Giant’ was achieved by culturing shoot tips in a 1/1 MS. The best medium in the introduction stage contained 2.0 mg/l BAP. Shoot proliferation occurred with 3.0 mg/1BAP. Some problems with callus and phenolic compounds appeared in both introduction and shoot multiplication stages. The plantlets were rooted either in 1/10 MS without hormones or directly in peat. Two months later most of ‘Black Satin’ and ‘Bedford Giant’ and about half of ‘Thornless Evergreen’ were growing steadily. Theoretically it is possible to produce 60,000 plants within a half year by this method.

Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers.

Science.gov (United States)

Akdemir, Hülya; Suzerer, Veysel; Tilkat, Engin; Onay, Ahmet; Çiftçi, Yelda Ozden

2016-12-01

Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in "reasonable polymorphism" among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.

Micropropagation of peach rootstocks and cultivars.

Science.gov (United States)

Balla, Ildikó; Mansvelt, Lucienne

2013-01-01

Peach (Prunus persica (L.) Batsch) is one of the most popular stone fruits, commercially produced largely in Mediterranean and, to a lesser extent, in continental climatic conditions. Several breeding programs with different aims release annually large numbers of new cultivars. Micropropagation offers a suitable method to provide the growers of sufficient quantities of rootstocks, as well as of pathogen-free planting material of old and new cultivars.An effective four-step micropropagation procedure for cultivar and rootstock production is described here, based on the use of modified MS and WPM media. The health status of the initial shoot tips is very important, also because the growth and proliferation rate of shoot cultures from virus-infected clones are generally very poor. Proliferation and elongation phases depend on the major macro-elements, as well as the content and ratio of plant growth regulators. It is important to grow the cultures at 22°C, as hyperhydricity may develop at higher temperatures. Although sucrose is the most common carbon source used during proliferation and rooting, for some peach cultivars and rootstocks the replacement of sucrose (10 g/L) with glucose (20 g/L) in the rooting medium improves the rooting and survival rates of plants in the acclimatization phase. The rooting rate of the rootstock "Cadaman" is improved with the chelated form of iron FeEDDHA at 150 mg/L. Rooted plants are acclimatized in greenhouse under high humidity conditions.

Influence of various carbohydrates on the in vitro micropropagation ...

African Journals Online (AJOL)

Sucrose appears to be the most favorable sugar to ensure the in vitro micropropagation of N. diderrichii. Sorbose caused the necrosis of explants in culture. The mannose and the mannitol, did not cause the necrosis of explants, but their presence had an unfavorable role on roots initiation and slow down the plants' growth.

Micropropagation of the endangered shrub pondberry (Lindera melissifolia [Walt.] Blume)

Science.gov (United States)

Tracy S. Hawkins; Nathan M. Schiff; Emile s. Gardiner; Theodore Leininger; Margaret S. Devall; A. Dan Wilson; Paul Hamel; Deborah D. McCown; Kristina Connor

2007-01-01

A micropropagation protocol using shoot cultures is described for Lindera melissifolia, a federally listed endangered shrub endemic to the southeastern United States. Stock plants were harvested from native L. melissifolia populations growing in the lower Mississippi Alluvial Valley. In vitro proliferation was on woody plant medium...

Micropropagation of Oenothera biennis L. and an assay of fatty acids

Directory of Open Access Journals (Sweden)

Lutosława Skrzypczak

2014-01-01

Full Text Available The species Oenothera biennis L. was micropropagated in in vitro cultures. After transferring to the ground plants developed correctly and generated fruits with seeds. The content of oil and γ-linolenic acid in seeds was determined.

Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers.

Science.gov (United States)

Siril, E A; Joseph, Nisha

2013-01-01

An in vitro propagation technique based on axillary bud proliferation was developed for the first time to mature annatto (Bixa orellana L.) tree. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with 1.0 μM benzyl adenine (BA) and tender coconut water (10 %) showed significantly high (P micropropagated plants. The present protocol in brief, can be used for the clonal propagation of the superior genotype and preservation of germplasm.

The role of topolins in micropropagation and somaclonal variation of banana cultivars ´Williams´ and ´Grand Naine´ (Musa spp. AAA)

Czech Academy of Sciences Publication Activity Database

Bairu, M. W.; Stirk, W.A.; Doležal, Karel; van Staden, J.

2008-01-01

Roč. 95, č. 3 (2008), s. 373-379 ISSN 0167-6857 R&D Projects: GA ČR GA522/06/0108 Institutional research plan: CEZ:AV0Z50380511 Keywords : Abnormality index * Banana * Micropropagation Subject RIV: ED - Physiology Impact factor: 1.017, year: 2008

In vitro micropropagation and mycorrhizal treatment influences the polyphenols content profile of globe artichoke under field conditions.

Science.gov (United States)

Pandino, Gaetano; Lombardo, Sara; Antonino, Lo Monaco; Ruta, Claudia; Mauromicale, Giovanni

2017-09-01

The commercial importance of plant tissue culture has grown in recent years, reflecting its application to vegetative propagation, disease elimination, plant improvement and the production of polyphenols. The level of polyphenols present in plant tissue is influenced by crop genotype, the growing environment, the crop management regime and the post-harvest processing practice. Globe artichoke is a significant component of the Mediterranean Basin agricultural economy, and is rich in polyphenols (phenolic acids and flavones). Most commercially grown plants are derived via vegetative propagation, with its attendant risk of pathogen build-up. Here, a comparison was drawn between the polyphenol profiles of conventionally propagated and micropropagated/mycorrhized globe artichoke plants. Micropropagation/mycorrhization appeared to deliver a higher content of caffeoylquinic acids. The accumulation of these compounds, along with luteolin and its derivatives, was not season-dependent. Luteolin aglycone was accumulated preferentially in the conventionally propagated plants. Overall, it appeared that micropropagation/mycorrhization enhanced the accumulation of polyphenols. Copyright © 2017. Published by Elsevier Ltd.

Physiological, vascular and nanomechanical assessment of hybrid poplar leaf traits in micropropagated plants and plants propagated from root cuttings: A contribution to breeding programs.

Science.gov (United States)

Ďurkovič, Jaroslav; Husárová, Hana; Javoříková, Lucia; Čaňová, Ingrid; Šuleková, Miriama; Kardošová, Monika; Lukáčik, Ivan; Mamoňová, Miroslava; Lagaňa, Rastislav

2017-09-01

Micropropagated plants experience significant stress from rapid water loss when they are transferred from an in vitro culture to either greenhouse or field conditions. This is caused both by inefficient stomatal control of transpiration and the change to a higher light intensity and lower humidity. Understanding the physiological, vascular and biomechanical processes that allow micropropagated plants to modify their phenotype in response to environmental conditions can help to improve both field performance and plant survival. To identify changes between the hybrid poplar [Populus tremula × (Populus × canescens)] plants propagated from in vitro tissue culture and those from root cuttings, we assessed leaf performance for any differences in leaf growth, photosynthetic and vascular traits, and also nanomechanical properties of the tracheary element cell walls. The micropropagated plants showed significantly higher values for leaf area, leaf length, leaf width and leaf dry mass. The greater leaf area and leaf size dimensions resulted from the higher transpiration rate recorded for this stock type. Also, the micropropagated plants reached higher values for chlorophyll a fluorescence parameters and for the nanomechanical dissipation energy of tracheary element cell walls which may indicate a higher damping capacity within the primary xylem tissue under abiotic stress conditions. The performance of the plants propagated from root cuttings was superior for instantaneous water-use efficiency which signifies a higher acclimation capacity to stressful conditions during a severe drought particularly for this stock type. Similarities were found among the majority of the examined leaf traits for both vegetative plant origins including leaf mass per area, stomatal conductance, net photosynthetic rate, hydraulic axial conductivity, indicators of leaf midrib vascular architecture, as well as for the majority of cell wall nanomechanical traits. This research revealed that

Micropropagation of PLUCHEA LANCEOLATA (Oliver

Directory of Open Access Journals (Sweden)

M. Kher Mafatlal

2014-09-01

Full Text Available Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962 - MS medium supple–mented with 6-benzylaminopurine (BAP, kinetin (Kin, thidiazuron (TDZ and 2iP (2-isopentenyladenine at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3. The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ. In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

Micropropagation of transgenic lettuce containing HBsAg as a method of mass-scale production of standardised plant material for biofarming purposes.

Science.gov (United States)

Pniewski, Tomasz; Czyż, Marcin; Wyrwa, Katarzyna; Bociąg, Piotr; Krajewski, Paweł; Kapusta, Józef

2017-01-01

Micropropagation protocol of transgenic lettuce bearing S-, M- and L-HBsAg was developed for increased production of uniformised material for oral vaccine preparation. Effective manufacturing of plant-based biopharmaceuticals, including oral vaccines, depends on sufficient content of a protein of interest in the initial material and its efficient conversion into an administrable formulation. However, stable production of plants with a uniformised antigen content is equally important for reproducible processing. This can be provided by micropropagation techniques. Here, we present a protocol for micropropagation of transgenic lettuce lines bearing HBV surface antigens: S-, M- and L-HBsAg. These were multiplied through axillary buds to avoid the risk of somaclonal variation. Micropropagation effectiveness reached 3.5-5.7 per passage, which implies potential production of up to 6600 plant clones within a maximum 5 months. Multiplication and rooting rates were statistically homogenous for most transgenic and control plants. For most lines, more than 90 % of clones obtained via in vitro micropropagation had HBsAg content as high as reference plants directly developed from seeds. Clones were also several times more uniform in HBsAg expression. Variation coefficients of HBsAg content did not exceed 10 % for approximately 40-85 % of clones, or reached a maximum 20 % for 90 % of all clones. Tissue culture did not affect total and leaf biomass yields. Seed production for clones was decreased insignificantly and did not impact progeny condition. Micropropagation facilitates a substantial increase in the production of lettuce plants with high and considerably equalised HBsAg contents. This, together with the previously reported optimisation of plant tissue processing and its long-term stability, constitutes a successive step in manufacturing of a standardised anti-HBV oral vaccine of reliable efficacy.

A Guide to Field Notes for Qualitative Research: Context and Conversation.

Science.gov (United States)

Phillippi, Julia; Lauderdale, Jana

2018-02-01

Field notes are widely recommended in qualitative research as a means of documenting needed contextual information. With growing use of data sharing, secondary analysis, and metasynthesis, field notes ensure rich context persists beyond the original research team. However, while widely regarded as essential, there is not a guide to field note collection within the literature to guide researchers. Using the qualitative literature and previous research experience, we provide a concise guide to collection, incorporation, and dissemination of field notes. We provide a description of field note content for contextualization of an entire study as well as individual interviews and focus groups. In addition, we provide two "sketch note" guides, one for study context and one for individual interviews or focus groups for use in the field. Our guides are congruent with many qualitative and mixed methodologies and ensure contextual information is collected, stored, and disseminated as an essential component of ethical, rigorous qualitative research.

IMPROVEMENTS OF CLONAL MICROPROPAGATION TECHNOLOGY OF APPLE ROOTSTOCKS AT THE STAGE OF INTRODUCTION TO IN VITRO CULTURE

OpenAIRE

Besedina E. N.; Buntsevich L. L.

2015-01-01

The stage of introduction of plantlets to in vitro culture is an important stage of technology of clonal micropropagation of plants. For the purpose of decrease in a share of the plantlets that were lost from an infection and increase of their regeneration ability, sterilizers and antibiotics, effective and safe for apple rootstocks’ plantlets wee allocated, and also favorable terms of introduction to in vitro culture were allocated. As a result of the conducted researches, we have establishe...

Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator.

Science.gov (United States)

Kalinina, Anna; Brown, Daniel C W

2007-07-01

A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 peach, commonly used for Prunus virus indexing. The micropropagation cycle based on proliferation of vegetative tissues includes establishment of tissue culture through introduction of shoot meristems in vitro, shoot proliferation, root induction and plant acclimatization steps and can be completed in 5 months. A meristem sterilization protocol minimized bacterial and fungal contamination. Multiple shoot formation in ornamental Prunus was obtained through the use of 1 mg l(-1) 6-benzyladenine. For GF305 peach, alteration in the sugar composition, fructose instead of sucrose, and addition of 1 mg l(-1 )ferulic acid had a significant impact on the shoot proliferation rate and maintenance of long-term in vitro culture. Rooting and plant acclimatization conditions were improved using a two-step protocol with a 4-day root induction in indole-3-butiric acid (IBA)-containing media with consequent 3-week root elongation in IBA-free media. One-month incubation of rooted shoots in a vermiculite-based medium resulted in additional shoot and root growth and provided better acclimatization and plant recovery. The micropropagation approach can be used for maintenance of the clonal properties for Prunus spp. as well as a protocol to support meristem therapy against viral infection.

Micropropagation of Ajuga bracteosa, a medicinal herb

OpenAIRE

Kaul, Shivanee; Das, Sandip; Srivastava, P. S.

2013-01-01

For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, l...

Micropropagation of Iris sp.

Science.gov (United States)

Jevremović, Slađana; Jeknić, Zoran; Subotić, Angelina

2013-01-01

Irises are perennial plants widely used as ornamental garden plants or cut flowers. Some species accumulate secondary metabolites, making them highly valuable to the pharmaceutical and perfume industries. Micropropagation of irises has successfully been accomplished by culturing zygotic embryos, different flower parts, and leaf base tissues as starting explants. Plantlets are regenerated via somatic embryogenesis, organogenesis, or both processes at the same time depending on media composition and plant species. A large number of uniform plants are produced by somatic embryogenesis, however, some species have decreased morphogenetic potential overtime. Shoot cultures obtained by organogenesis can be multiplied for many years. Somatic embryogenic tissue can be reestablished from leaf bases of in vitro-grown shoots. The highest number of plants can be obtained by cell suspension cultures. This chapter describes effective in vitro plant regeneration protocols for Iris species from different types of explants by somatic embryogenesis and/or organogenesis suitable for the mass propagation of ornamental and pharmaceutical irises.

Micropropagation of Alstroemeria in liquid medium using slow release of medium components

NARCIS (Netherlands)

Klerk, de G.J.M.; Brugge, ter J.

2010-01-01

Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous

Micropropagation of Myriophyllum alterniflorum (Haloragaceae) for stream rehabilitation: first in vitro culture and reintroduction assays of a heavy-metal hyperaccumulator immersed macrophyte.

Science.gov (United States)

Delmail, David; Labrousse, Pascal; Hourdin, Philippe; Larcher, Laure; Moesch, Christian; Botineau, Michel

2013-01-01

Nowadays, submersed aquatic macrophytes play a key role in stream ecology and they are often used as biomonitors of freshwater quality. So, these plants appear as natural candidates to stream rehabilitation experiments. Among them, the stream macrophyte Myriophyllum alterniflorum is used recently as biomonitor and is potentially useful for the restoration of heavy-metal contaminated localities. The best way to obtain a mass production of watermilfoil plants is micropropagation. We developed in vitro culture of M. alterniflorum and the effects of five media on the plant development were assessed. Five morphological and four physiological endpoints were examined leading to the recommendation of the Murashige and Skoog medium for ecotoxicological studies on chlorophyllous parts, and of the Gaudet medium for root cytotoxicity and phytoremediation studies. Micropropagated clones were acclimatized in a synthetic medium and in situ reintroduction was performed efficiently. This is the first report of micropropagated plants transplantation in streams. The successful establishment of watermilfoil beds even in polluted areas strongly suggested that ecological restoration using micropropagated watermilfoil is a promising biotechnology for phytoremediation and rehabilitation of degraded areas. Moreover, high bioconcentration factors evidenced that watermilfoil hyperaccumulates Cd and Cu, and could be potentially used in phytoremediation studies.

Micropropagation to Rescue Endangered Plant Moringa Concanensis Nimmo (Moringaceae)

International Nuclear Information System (INIS)

Fatima, H.

2016-01-01

Efficient micropropagation was developed for an endangered plant; Moringa concanensis Nimmo. The plant has many medicinal properties. It is an antioxidant, anti-diabetic and a powerful tonic alternative. A high frequency and maximum number of shoots were produced in MS supplemented with the combination of Benzyl aminopurine (BAP) and Alpha-Naphthalene Acetic Acid (NAA) (0.10mg/l + 0.05mg/l). Rooting was achieved by the inoculation of regenerated shoots on half strength MS without addition of hormone. (author)

Micropropagation of Ficus religiosa L. via leaf explants and comparative evaluation of acetylcholinesterase inhibitory activity in the micropropagated and conventionally grown plants.

Science.gov (United States)

Siwach, Priyanka; Gill, Anita Rani

2014-10-01

A high-frequency, season-independent, in vitro regeneration of Ficusreligiosa was developed, followed by comparative acetylcholinesterase inhibitory (AChEI) activity assay of the in vitro raised and conventionally grown plants. The use of AChEI activity is the most accepted strategy for the treatment of Alzheimer disease. Fully expanded, mature leaves were cut into different segments to initiate the cultures. The middle section of the leaf in vertical orientation with cut portion inserted inside the medium was found most suitable for direct shoot regeneration. Leaf explants responded with nearly consistent frequency (60-66.67 %) throughout the year. To obtain high frequency response with enhanced shoot multiplication rate, 32 plant growth regulator regimes were screened amongst which benzylaminopurine at 5.0 mg/l was found most suitable, yielding 100 % response and maximum number of shoots per explant (7.93); same concentration was also most supportive for repeated multiplication (6.53 shoots). The quality of the shoots and multiplication rate could be significantly enhanced (24.35 shoots) when adenine sulphate, glutamine and phloroglucinol, in an optimised concentration, were additionally supplemented. The clonal nature of the micropropagated plants was confirmed by random amplified polymorphic DNA analysis. A comparative analysis of AChEI activity was carried out amongst the methanolic extracts of stem segments of the mother plant, randomly selected seedlings of different age (4 and 6 months old) of the same mother plant and randomly selected micropropagated plants of different age (3 and 6 months age). The mother plant sample showed effective AChEI activity, with IC 50 of 66.46 μg/ml while seedlings, of different age groups, performed poorly (6-month-old seedlings, Se-1 6M , yielded IC 50 of 20,538.46 μg/ml, while two randomly selected 4 months' aged seedlings, Se-2 4M and Se-3 4M exhibited IC 50 of 19,341.03 and 24,281.70 μg/ml). On the other hand

Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia.

Science.gov (United States)

Muniz, Aleksander Westphal; de Sá, Enilson Luiz; Dalagnol, Gilberto Luíz; Filho, João Américo

2013-01-01

In vitro rooting and the acclimatization of micropropagated rootstocks of apple trees is essential for plant development in the field. The aim of this work was to assess the use of rhizobia of Adesmia latifolia to promote rooting and acclimatization in micropropagated Marubakaido apple rootstock. An experiment involving in vitro rooting and acclimatization was performed with four strains of rhizobium and two controls, one with and the other without the addition of synthetic indoleacetic acid. The inoculated treatments involved the use of sterile inoculum and inoculum containing live rhizobia. The most significant effects on the rooting rate, primary-root length, number of roots, root length, fresh-shoot biomass, and fresh-root biomass were obtained by inoculation with strain EEL16010B and with synthetic indole acetic acid. However, there was no difference in the growth of apple explants in the acclimatization experiments. Strain EEL16010B can be used to induce in vitro rooting of the Marubakaido rootstock and can replace the use of synthetic indoleacetic acid in the rooting of this cultivar.

The influence of fast neutron irradiation through micropropagation, calli induction and cell aggregate suspension culture of tapak dara cultivate vinca rosea linn

International Nuclear Information System (INIS)

Wahid, Rosmiarty A.

2000-01-01

Study on the influence of fast neutron irradiation toward tissue induction of apical shoot, calli of leaf and corolla as well as development of bud micropropagation using variety of MS and Gamborg (B5) which were supplemented with growth hormone 2,4-D NAA, BAP and kinetin has been carried out. Cell aggregates were obtained from modified liquid media by mixing MS macro element and Gamborg vitamin. Influence of the iow level irradiation (0,5-10 Gy) was investigated for auxiliary bud micropropagation , middle (5 - 20 Gy) for calli induction, while for call aggregates higher doses (until 30 Gy) were used. Optimum growth of bud micropropagation was stimulate at dose range between 0,5-1Gy and grown on MS supplemented whit BAP and NAAN, while for leaf and corolla calli was at 5 Gy, on MS media which was supplemented whit 1 mg/L kinetin, 10mg/L BAP and 0,5 mg/L NAA. However, neutron dose of 10 Gy decreased the induction of leaf and corolla calli. The highest radioresistance was shown by cell aggregates of leaf calli that grew prosperously up to 20Gy. Key words : fast neutron, micropropagation, tissue culture, cell culture, vinca rosea L

Temporary Immersion System for Date Palm Micropropagation.

Science.gov (United States)

Othmani, Ahmed; Bayoudh, Chokri; Sellemi, Amel; Drira, Noureddine

2017-01-01

The temporary immersion system (TIS) is being used with tremendous success for automation of micropropagation of many plant species. TIS usually consists of a culture vessel comprising two compartments, an upper one with the plant material and a lower one with the liquid culture medium and an automated air pump. The latter enables contact between all parts of the explants and the liquid medium by setting overpressure to the lower part of the container. These systems are providing the most satisfactory conditions for date palm regeneration via shoot organogenesis and allow a significant increase of multiplication rate (5.5-fold in comparison with that regenerated on agar-solidified medium) and plant material quality, thereby reducing production cost.

Research Note: Inside an Indonesian Online Library for Radical Materials

Directory of Open Access Journals (Sweden)

Muhammad Haniff Hassan

2012-12-01

Full Text Available This Research Note provides a review of an Indonesian online library for radical materials. The objective of this review is to compile data and information that will contribute to the understanding of the online radicalisation phenomenon as well as the extremists themselves. Based on data found on the online library, this Research Note reports findings on the influence of Al-Maqdisi’s website; the emphasis on translation work of Arabic materials to Indonesian language by radicals and the value of Arabic materials to them. It also covers influential thinkers and ideologues and the use of the Wikipedia modus operandi to hasten the development of the website and effect mobilisation and recruitment, among others things. Based on the data found, this Research Note concludes that ideas matter to radicals.

Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.

Science.gov (United States)

Vidal, Á M; Vieira, L J; Ferreira, C F; Souza, F V D; Souza, A S; Ledo, C A S

2015-07-14

Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard's index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.

Use of plastic films for weed control during field establishment of micropropagated hardwoods

Science.gov (United States)

J. W. Van Sambeek; John E. Preece; Carl A. Huetteman; Paul L. Roth

1995-01-01

This study compares the use of plastic films to conventional methods for establishing hardwoods on a recently cultivated old field site using 1-year-old micropropagated plantlets of white ash (Fraxinus americana L.) and silver maple (Acer saccharinum L.). After one growing season in the field, height of plantlets with all weed...

Somatic embryogenesis for efficient micropropagation of guava (Psidium guajava L.).

Science.gov (United States)

Akhtar, Nasim

2013-01-01

Guava (Psidium guajava L.) is well known for edible fruit, environment friendly pharmaceutical and commercial products for both national and international market. The conventional propagation and in vitro organogenesis do not meet the demand for the good quality planting materials. Somatic embryogenesis for efficient micropropagation of guava (P. guajava L.) has been developed to fill up the gap. Somatic embryogenesis and plantlets regeneration are achieved from 10-week post-anthesis zygotic embryo explants by 8-day inductive treatment with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) on MS agar medium containing 5% sucrose. Subsequent development and maturation of somatic embryos occur after 8 days on MS basal medium supplemented with 5% sucrose without plant growth regulator. The process of somatic embryogenesis shows the highest relative efficiency in 8-day treatment of zygotic embryo explants with 1.0 mg L(-1) 2,4-D. High efficiency germination of somatic embryos and plantlet regeneration takes place on half strength semisolid MS medium amended with 3% sucrose within 2 weeks of subculture. Somatic plantlets are grown for additional 2 weeks by subculturing in MS liquid growth medium containing 3% sucrose. Well-grown plantlets from liquid medium have survived very well following 2-4 week hardening process. The protocol of somatic embryogenesis is optimized for high efficiency micropropagation of guava species.

Research Note: Comparative antibacterial activities of oil-palm ...

African Journals Online (AJOL)

Research Note: Comparative antibacterial activities of oil-palm Elaeis ... The antimicrobial activities liquid pyrolysates (obtained by destructive distillation), their ... respective chloroform fractions which showed higher activities than the crude ...

Microbiological and Pharmacological Evaluation of the Micropropagated Rubus liebmannii Medicinal Plant

Directory of Open Access Journals (Sweden)

Adelina Jiménez-Arellanes

2012-01-01

Full Text Available Rubus liebmannii is an endemic species from Mexico used in traditional medicine primarily to treat dysentery and cough. The in vitro activity against Giardia lamblia and Entamoeba histolytica that produces the ethanolic extract of the aerial parts of the plant led us to expand the pharmacological and phytochemical research of this species. Gastrointestinal disorders including amebiasis remain one of the health problems that need to be addressed and it is of interest to find alternatives that improve their treatment. Also, it is important to emphasize that R. liebmannii grows wild in the country and is not found in abundance; therefore, alternatives that avoid overexploitation of the natural resource are mandatory. Ongoing with the evaluation of the potentialities that R. liebmannii possesses for treating infectious gastrointestinal diseases, the aim of the present study was to evaluate the biological effects and the chemical composition of the micropropagated plant.

Micropropagation and maintenance of phytoplasmas in tissue culture.

Science.gov (United States)

Bertaccini, Assunta; Paltrinieri, Samanta; Martini, Marta; Tedeschi, Mara; Contaldo, Nicoletta

2013-01-01

Maintenance of phytoplasma strains in tissue culture is achievable for all strains transmitted to periwinkle (Catharanthus roseus), and also for other naturally infected plant host species. Shoots of 1-3 cm length are grown in a solid medium containing Murashige and Skoog (MS) micro- and macroelements and 0.12 mg/L benzylaminopurine. The continued presence of phytoplasmas in infected shoots of periwinkle that have been maintained in micropropagation for up to 20 years can be shown by diagnostic methods such as nested PCR tests using the 16S rDNA gene (see Chapters 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,and 26 for phytoplasma diagnostic methods).

An improved micropropagation of Arnebia hispidissima (Lehm.) DC. and assessment of genetic fidelity of micropropagated plants using DNA-based molecular markers.

Science.gov (United States)

Phulwaria, Mahendra; Rai, Manoj K; Shekhawat, N S

2013-07-01

An efficient and improved in vitro propagation method has been developed for Arnebia hispidissima, a medicinally and pharmaceutically important plant species of arid and semiarid regions. Nodal segments (3-4 cm) with two to three nodes obtained from field grown plants were used as explants for shoot proliferation. Murashige and Skoog's (MS) medium supplemented with cytokinins with or without indole-3-acetic acid (IAA) or naphthalene acetic acid was used for shoot multiplication. Out of different PGRs combinations, MS medium containing 0.5 mg l(-1) 6-benzylaminopurine and 0.1 mg l(-1) IAA was optimal for shoot multiplication. On this medium, explants produced the highest number of shoots (47.50 ± 0.38). About 90 % of shoots rooted ex vitro on sterile soilrite under the greenhouse condition when the base (2-4 mm) of shoots was treated with 300 mg l(-1) of indole-3-butyric acid for 5 min. The plantlets were hardened successfully in the greenhouse with 85-90 % survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro-regenerated plants of A. hispidissima. Out of 40 (25 RAPD and 15 ISSR) primers screened, 15 RAPD and 7 ISSR primers produced a total number of 111 (77 RAPD and 34 ISSR) reproducible amplicons. The amplified products were monomorphic across all the micropropagated plants and were similar to the mother plant. To the best of our knowledge, it is the first report on the assessment of the genetic fidelity in micropropagated plants of A. hispidissima.

Organic amendments enhance Pb tolerance and accumulation during micropropagation of Daphne jasminea.

Science.gov (United States)

Wiszniewska, Alina; Muszyńska, Ewa; Hanus-Fajerska, Ewa; Smoleń, Sylwester; Dziurka, Michał; Dziurka, Kinga

2017-01-01

The study investigated the effects of organic amendments: pineapple pulp (PP) and agar hydrolyzate (AH), on micropropagation and Pb bioaccumulation and tolerance in a woody shrub Daphne jasminea cultured in vitro. The amendments were analyzed for their content of carbohydrates, phenolic acids, and phytohormones and added at a dose of 10 mL L -1 to the medium containing 1.0 mM lead nitrate. Micropropagation coefficient increased by 10.2-16.6 % in PP and AH variants, respectively. Growth tolerance index increased by 22.9-31.8 % for the shoots and by 60.1-82.4 % for the roots. In the absence of Pb, the additives inhibited multiplication and growth of microplantlets. PP and AH facilitated Pb accumulation in plant organs, especially in the roots. PP enhanced bioconcentration factor and AH improved Pb translocation to the shoots. Adaptation to Pb was associated with increased accumulation of phenolics and higher radical scavenging activity. Medium supplementation, particularly with AH, enhanced antiradical activity of Pb-adapted lines but reduced the content of phenolic compounds. The study results indicated that supplementation with organic amendments may be beneficial in in vitro selection against lead toxicity.

Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.

Directory of Open Access Journals (Sweden)

Mary E Lucero

2011-03-01

Full Text Available Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP analysis amplified sequences from callus homologous to diverse fungal and bacterial taxa. Culturing isolated some seed borne endophyte taxa which could be readily propagated apart from the host. Microbial cells were observed within biofilm-like residues associated with plant cell surfaces and intercellular spaces. Various universal primers amplified both plant and microbial sequences, with different primers revealing different patterns of fungal diversity. Bacterial and fungal TEFAP followed by alignment with sequences from curated databases revealed 7 bacterial and 17 ascomycete taxa in A. canescens, and 5 bacterial taxa in A. torreyi. Additional diversity was observed among isolates and clone libraries. Micropropagated Atriplex retains a complex, intimately associated microbiome which includes diverse strains well poised to interact in manners that influence host physiology. Microbiome analysis was facilitated by high throughput sequencing methods, but primer biases continue to limit recovery of diverse sequences from even moderately complex communities.

Reducing Bias: Research Notes on Racism in America.

Science.gov (United States)

Mabbutt, Richard

This paper highlights recent developments in research on racism in the United States, and notes several conceptual issues of significance for the long-range planning work of those interested in reducing racism in America and particularly in Idaho. Growth in the number of minority researchers has resulted in increased attention toward racism as it…

How does exogenously applied cytokinin type affect growth and endogenous cytokinins in micropropagated Merwilla plumbea?

Czech Academy of Sciences Publication Activity Database

Aremu, A.O.; Plačková, Lenka; Bairu, M. W.; Novák, Ondřej; Plíhalová, Lucie; Doležal, Karel; Finnie, J. F.; van Staden, J.

2014-01-01

Roč. 118, č. 2 (2014), s. 245-256 ISSN 0167-6857 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : Conservation * Medicinal plant * Micropropagation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.125, year: 2014

Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study.

Science.gov (United States)

Badalamenti, Ornella; Carra, Angela; Oddo, Elisabetta; Carimi, Francesco; Sajeva, Maurizio

2016-01-01

Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Ehrenberg, an Appendix I CITES listed species critically endangered and sought after by the horticultural trade, on micropropagated Opuntia ficus-indica Miller. Apical and sub-apical scions of P. aselliformis were used to perform micrografting with a successful rate of 97 and 81 % respectively. Survival rate after ex vivo transfer was 85 %. We hypothesize that this method could be applied to other endangered, slow growing taxa of Cactaceae thus contributing to the conservation of this endangered family.

Optimization of a protocol for the micropropagation of pineapple

Directory of Open Access Journals (Sweden)

ALMEIDA WELITON ANTONIO BASTOS DE

2002-01-01

Full Text Available The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L. Merrill cv. Pérola. Changes in benzylaminopurine (BAP concentration, type of medium (liquid or solidified and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%, for three minutes, followed by calcium hypochlorite (2%, for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1, 27 ± 2ºC. After eight weeks, cultures were subcultured for multiplication in MS medium. The following treatments were tested: liquid x solidified medium with different BAP concentrations (0.0, 1.5 or 3.0 mg L-1, and the longitudinal cut, or not, of the shoot bud used as explant. The results showed that liquid medium supplemented with BAP at 1.5 mg L-1, associated with the longitudinal sectioning of the shoot bud used as explant presented the best results, maximizing shoot proliferation. On average, the best treatment would allow for an estimated production of 161,080 plantlets by the micropropagation of the axillary buds of one plant with eight slips and ten buds/slips, within a period of eight months.

In vitro micropropagation of chive (Allium schoenoprasum L.

Directory of Open Access Journals (Sweden)

Marek Lubomski

2013-12-01

Full Text Available The investigations on micropropagation of Allium schoenoprasum embraced shoot formation from cultured shoot tips, shoot multiplication, root formations, and cold storage of rooted plants. All explants were cultured on modified Murashige-Skoog medium. Surface disinfection of shoots derived from stock plants was not necessary to obtain no infection culture. The highest shoot multiplication was obtained on medium with 20 g l-1 sucrose and 1.0 mg 1-1 6-benzylo-arninopurine (BA. Root formation was observed in both indoleacetic acid (IAA and indolebutyric acid (IBA. Rooted plants were successfully kept in cold storage (5°C ± 1°C darkness. Only 20% of plants died after 6 months of storage.

Micropropagation of Plantago asiatica L. through culture of shoot-tips

Directory of Open Access Journals (Sweden)

Joanna Makowczyńska

2011-01-01

Full Text Available Shoot-tip multiplication of the medicinal species - Plantago asiatica was carried on MS medium with IAA and BAP or kinetin. Best results in micropropagation were achieved by adding 0.1 mg/dm3 IAA and 1 mg/dm3 BAP. After 6 weeks shoots were transferred to MS medium for rooting. The resulting plantlets were transferred after 8 weeks into pots and after a period of adaptation into the ground (field culture. The species Plantago asiatica was propagated in vitro by shoot-tip multiplication for the first time.

Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

Directory of Open Access Journals (Sweden)

Aurilena de Aviz Silva

Full Text Available ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 11711, and Herbaspirillum seropedicae HRC 54, five levels of urea (0, 2, 5, 7, and 10g L-1, and five evaluation periods (30, 60, 90, 120, and 150 days following the planting of plantlets in trays, and with four replications of each treatment. An increase in the dried weight of shoots and roots of micropropagated pineapple cv. 'Vitória' plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer.

Rooting of hybrid clones of Populus tremula L. x P. tremuloides Michx. by stem cuttings derived from micropropagated plants

Energy Technology Data Exchange (ETDEWEB)

Qibin Yu [Univ. of Helsinki (Finland). Dept. of Plant Biology; Maentylae, N. [Univ. of Turku (Finland). Dept. of Biology, Plant Physiology and Molecular Biology; Salonen, M. [Finnish Forest Research Inst., Laeyliaeinen (Finland). Haapastensyrjae Breeding Station

2001-07-01

Propagation costs could be cut by replacing part of the micropropagation process with steps involving more traditional techniques. This study explored possibilities for improving existing vegetative propagation techniques for aspen using stem cuttings obtained from micropropagated plants. Vegetative propagation through stem cuttings was studied in 10 micropropagated hybrid aspen clones (Populus tremula L. x P. tremuloides Michx). Cuttings containing one axillary bud were harvested from the same donor plants twice during the growing season: the first harvest in May and the second harvest in July. Rooting percentage was correlated positively with root length, number of roots and height of cutting plant but negatively with length of rooting. The average rooting percentage was 53% in the first harvest and 27% in second harvest. Indole-3-butyric acid treatments (1.2 mM) significantly improved rooting in the second harvest, but not in the first harvest, suggesting different endogenous auxin levels in the cuttings. A significant variation for most traits related to rooting ability was found among the clones, indicating that clonal effects play an important role in the propagation of aspen. Thus, clones with a good response in shoot growth and rooting could be exploited in large-scale propagation using stem cuttings.

Acclimatization and leaf anatomy of micropropagated fig plantlets

Directory of Open Access Journals (Sweden)

Chrystiane Fráguas Chirinéa

2012-12-01

Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.

Automatic micropropagation of plants--the vision-system: graph rewriting as pattern recognition

Science.gov (United States)

Schwanke, Joerg; Megnet, Roland; Jensch, Peter F.

1993-03-01

The automation of plant-micropropagation is necessary to produce high amounts of biomass. Plants have to be dissected on particular cutting-points. A vision-system is needed for the recognition of the cutting-points on the plants. With this background, this contribution is directed to the underlying formalism to determine cutting-points on abstract-plant models. We show the usefulness of pattern recognition by graph-rewriting along with some examples in this context.

Plant abiotic stress tolerance analysis in cauliflower using a curd micropropagation system

OpenAIRE

Rihan, HZ; Al-Issawi, M; Al-Shamari, M; Elmahrouk, M; Fuller, MP

2015-01-01

© 2015 ISHS. An effective protocol for cauliflower micropropagation was optimised and developed which enabled the production of tens of thousands of cauliflower microshoots from one cauliflower curd. The large number of microshoots that can be produced per culture unit facilitates the use of this protocol to analyse both the physiological and molecular components of abiotic stress tolerance. The protocol was used for cauliflower cold tolerance analysis and it was demonstrated that low tempera...

Research Paper:

African Journals Online (AJOL)

Shanu

2014-01-08

Jan 8, 2014 ... Forty years in capsaicin research for sensory pharmacology and physiology. Neuropeptides 38:377-384. Thomas E (2002). Tissue culture studies in Arachis hypogea L. and. Vignaunguiculata (L.) Walp. for micropropagation and cell line selection for amino acid overproduction, Ph.D. Thesis, University of.

Métodos de micropropagação de abacaxizeiro Micropropagation methods of pineapple

Directory of Open Access Journals (Sweden)

Adriano Bortolotti da Silva

2007-09-01

Full Text Available O objetivo deste trabalho foi comparar diferentes métodos de micropropagação in vitro de abacaxizeiro em larga escala. Os tratamentos utilizados foram micropropagação convencional em meio sólido (5 e 6 g L-1 de ágar e em meio líquido, e micropropagação em biorreator de imersão temporária - imersão no meio de cultura a cada 2, 4 e 8 horas, por 3 min - e contínua, com aeração a cada 2, 4 e 8 horas, por 3 min. Utilizou-se meio MS suplementado com 1 mg L-1 de BAP, 0,25 mg L-1 de ANA, pH ajustado para 5,8. As culturas foram mantidas em sala de crescimento com 25±1ºC, sob luz branca fria (40 µmol m-2 s-1 , com 16 horas de fotoperíodo. O delineamento foi inteiramente casualizado, com nove tratamentos e quatro repetições. Depois de 45 dias de cultivo, foram avaliados número de brotos, brotações superiores a 1 cm, comprimento de brotos, massa de matéria fresca e massa de matéria seca de brotos. Sistemas de imersão temporária, com as plântulas imersas a cada 2 horas por 3 min, proporcionaram maior número, altura e massa de matéria seca de brotos de abacaxizeiro. O sistema de imersão temporária é o método mais eficiente na micropropagação de abacaxizeiro em larga escala.The objective of this work was to compare different methods of pineapple in vitro mass micropropagation. The treatments were conventional micropropagation in solid medium (5 and 6 g L-1 agar and in liquid medium, micropropagation in bioreactor of temporary immersion (immersion in culture medium each 2, 4 and 8 hours, for 3 minutes and continuous immersion (with aeration each 2, 4 and 8 hours, for 3 minutes. MS medium supplement with 1 mg L-1 BAP, 0.25 mg L-1 NAA, and pH adjusted to 5.8 was utilized. Cultures were incubated at 25±1ºC under cool white fluorescent tubes (40 µmol m-2 s-1 with a 16-hours photoperiod. The experiment design was completely randomized, comprising nine treatments and four replicates. After 45 days, number of shootings

Research Note Nutritional evaluation of Ficus thonningii Blume ...

African Journals Online (AJOL)

Research Note Nutritional evaluation of Ficus thonningii Blume leaves as ruminant livestock feed in the Ahferom district of Tigray, Ethiopia. ... Data on foliar nitrogen (N), phosphorus (P), potassium (K), dry matter (DM), crude protein (CP), digestible crude protein (DCP), ether extract (EE), crude fibre (CF), ash content and ...

Endogenous cytokinin dynamics in micropropagated tulips during bulb formation process influenced by TDZ and iP pretreatment

Czech Academy of Sciences Publication Activity Database

Podwyszynska, M.; Novák, Ondřej; Doležal, Karel; Strnad, Miroslav

2014-01-01

Roč. 119, č. 2 (2014), s. 331-346 ISSN 0167-6857 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : Tulipa gesneriana * Endogenous cytokinins * Micropropagation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.125, year: 2014

Response of Opuntia stricta Haw micropropagated plants in acclimatization and field

Directory of Open Access Journals (Sweden)

Marina Medeiros de Araújo Silva

2017-07-01

Full Text Available Forage palm (Opuntia stricta Haw. is a great important crop for the semi-arid region of Brasil. It has been propagated through in vitro culture, by areole activation. This work aimed to determine the response of Opuntia stricta Haw. variety ‘Oreja de Elefante Mexicana’ micropropagated plants during its acclimatization and field planting. Plants were acclimatized in tubes and plastic bags with a mixture of soil and manure, and it were kept in greenhouse for 130 days. Later, the plants kept in tubes were divided into nine treatments, according to their size (between 6 to 14 cm, and transfer to the field. The use of tubes provided a higher survival, height, fresh and dry mass of the cladodes. There was no significant differences between the recipients used for root development. At the end of acclimatization it was observed that plants did not have a uniform development, presenting size variation. After the first 15 days in the field, the majority of the plants with primary shoots were larger than 10 cm. At 60 days the survival was 100%, and all plants presented shoots, even the smallest. However, is recommended that plants larger than 10 cm will be used, due to the better establishment to field conditions. The results demonstrate the feasibility in the use of micropropagated plants for the establishment of forage palm fields. Keywords: cladodes, forage palm, in vitro culture, semi-arid region

Agricultural biotechnology research and development in Ethiopia

African Journals Online (AJOL)

PRECIOUS

2009-12-29

Dec 29, 2009 ... Review. Agricultural biotechnology research and development in Ethiopia ... seed micropropagation, virus-cleaning ongoing, good progress. Garlic meristem ... large quantities of disease-free planting materials in short time.

Micropropagation of Ajuga bracteosa, a medicinal herb.

Science.gov (United States)

Kaul, Shivanee; Das, Sandip; Srivastava, P S

2013-04-01

For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, leaf displayed quickest response followed by petiole while root was the slowest. Hardening of plantlets was achieved with 82 % survival. The hardened plants were maintained in pots with garden soil under controlled (Temp. 25 ± 2 °C) conditions. RAPD exhibited genetic fidelity with 100 % monomorphism in regenerants.

Effect of Silver Nitrate DuringEx vitro Acclimatization of Micropropagated Ginger Cultivars

Directory of Open Access Journals (Sweden)

Dikash Singh THINGBAIJAM

2014-03-01

Full Text Available Silver nitrate (AgNO3 was used under in vitro conditions to study the response of ginger cultivars ‘Nadia’ and ‘Baishey’ under ex vitro. Micropropagated plants treated with AgNO3 showed significant difference (p<0.05 compared to those plantlets without AgNO3 and control type in almost all the different quantitative traits analyzed. Significant difference in number of finger per plant and minirhizome yield indicated the repercussion of AgNO3 during acclimatization.

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop.

Science.gov (United States)

Choudhary, Sumitra Kumari; Patel, Ashok Kumar; Harish; Shekhawat, Smita; Shekhawat, Narpat S

2017-07-01

Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L -1 BAP, 0.1 mg L -1 IAA and additives (50 mg L -1 ascorbic acid, 25 mg L -1 each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L -1 IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female M. dioica , a popular summer vegetable crop.

Micropropagation of six Paulownia genotypes through tissue culture

Directory of Open Access Journals (Sweden)

Lydia Shtereva

2014-12-01

Full Text Available We investigated the effect of genotype and culture medium on the in vitro germination and development of plantlets from seeds of 6 different Paulownia genotypes (P. tomentosa, hybrid lines P. tomentosa P. fortunei (Mega, Ganter and Caroline, P. elongata and hybrid line P. elongata P. fortunei. Nodal and shoot tip explants were used for micropropagation of Paulownia genotypes by manipulating plant growth regulators. The highest germination percentage for all genotypes was obtained for seeds inoculated on medium supplemented with 50 mg*L GA3 (MSG2. On Thidiazuron containing media, the explants of hybrid line P. elongata P. fortunei exhibited the highest frequency of axillary shoot proliferation following by P. tomentosa P. fortunei. The results are discussed with the perspective of applying an improved protocol for in vitro seed germination and plantlet formation in several economically valuable Paulownia genotypes.

Call for concept notes for research on gender equality and ...

International Development Research Centre (IDRC) Digital Library (Canada)

2017-08-07

Aug 7, 2017 ... Call for concept notes for research on gender equality and decentralization in Myanmar ... address gender inequality in political participation; and; improve gender-sensitive ... ROSSA's latest bulletin puts a focus on women.

Note by Note: a New Revolution in Cooking

OpenAIRE

Burke, Roisin; Danaher, Pauline

2016-01-01

Note by note cooking is an application of Molecular Gastronomy. It was first proposed by French Physical Chemist and Molecular Gastronomy Co-founder, Hervé This. Note by Note dishes are being created as part of Ph.D. research in the Dublin Institute of Technology, Cathal Brugha Street.

Micropropagation and cytogenetic assessment of Zingiber species of Northeast India.

Science.gov (United States)

Das, Archana; Kesari, Vigya; Rangan, Latha

2013-12-01

An improved micropropagation protocol was developed for Zingiber moran and Z. zerumbet, two wild species of the genus Zingiber, found in Northeast India. The effects of growth regulators, sugar concentrations, and nutrients were tested on the rate of shoot initiation and multiplication. An increase in proliferation and multiplication occurred in modified Murashige and Skoog (MS) medium supplemented with benzyladenine and kinetin. About 2 % sucrose and 0.7 % agar were found to be the optimum for shoot multiplication and regeneration. Naphthalene acetic acid at 0.5 mg/L produced the best rooting response for both the species. Regenerated plantlets were acclimatized successfully and cytogenetic stability was confirmed by RAPD profiling and ploidy checks.

Environmental accounting and reporting of energy utility companies. Research notes

International Nuclear Information System (INIS)

Heiskanen, E.; Heininen, M.; Heurlin, E.; Lovio, R.; Paenkaelaeinen, M.

1997-09-01

The research note consists of articles written by a number of authors. The aim of the articles is to describe general development trends of environmental accounting and reporting from the point of view of the energy sector

Micropropagation of Jujube (Ziziphus jujuba

Directory of Open Access Journals (Sweden)

A. Khazaei

2016-02-01

Full Text Available Introduction: Jujube (Ziziphus jujuba is one of the most important fruit trees in Asia which has been planted from 3,000 years ago in China for medicinal purposes. Jujube belongs to the Rhamnaceae family. The Jujube fruit is used in fresh and dry forms. The fruit is full of vitamin C and has anticancer and medicinal effects. This tree can grow on salty and dry lands in Iran. Therefore, increasing the cultivation area of Jujube can be effective for soil conservation. In the last 20years, cultivation of Jujube is is considerable in Iran specially in the South Khorasan Province and 98 % of total production of Jujube in Iran belongs to this province. The low rate of seed germination and low production of shootlets are the most important problems in Jujube proliferation, so micropropagation of this plant through tissue culture was considered. Materials and methods: In this study, Cangan ecotype of Jujube was used for multiple shoot regeneration. At the end of May, apical buds of shoots were cut from mature trees of the Research Collection of Jujube at Sarbishe, Birjand, South Khorasan Province in Iran. The buds were disinfected with 70% ethanol for 1 min and 2% sodium hypoclorite for 25 min. Then the buds were rinsed with distilled water for 25 min completely. Apical buds were placed on Murashige and Skoog (MS medium supplemented with different concentrations of BA (0.5, 1, 1.5, 2 mg/L in combination with IBA or NAA (0, 0.1, 0.2, 0.4 mg/L. After one month, the shoots with 3-5 cm length were transferred to rooting media (1/2 MS + IBA or IAA : 0.5, 2, 5, 10 mg/L. The data were recorded after shooting and rooting and were analysed in the facorial experiment. Results and Discussion: The results of variance analysis and mean comparisons showed that there are differences between different levels of IBA and BA alone for the number of shoots and their length (P

Micropropagation of Citrus spp. by organogenesis and somatic embryogenesis.

Science.gov (United States)

Chiancone, Benedetta; Germanà, Maria Antonietta

2013-01-01

Citrus spp., the largest fruit crops produced worldwide, are usually asexually propagated by cuttings or grafting onto seedling rootstocks. Most of Citrus genotypes are characterized by polyembryony due to the occurrence of adventive nucellar embryos, which lead to the production of true-to-type plants by seed germination. Tissue culture and micropropagation, in particular, are valuable alternatives to traditional propagation to obtain a high number of uniform and healthy plants in a short time and in a small space. Moreover, in vitro propagation provides a rapid system to multiply the progeny obtained by breeding programs, allows the use of monoembryonic and seedless genotypes as rootstocks, and it is very useful also for breeding and germplasm preservation.In this chapter, two protocols regarding organogenesis of a rootstock and somatic embryogenesis of a cultivar have been described.

Micropropagation of Helleborus through axillary budding.

Science.gov (United States)

Beruto, Margherita; Viglione, Serena; Bisignano, Alessandro

2013-01-01

Helleborus genus, belonging to the Ranunculaceae family, has 20 species of herbaceous perennial flowering plants. The commercial exploitation of this plant is dependent on the selection and propagation of appropriate lines. High propagation rate could be accomplished by using a suitable tissue culture method enabling the rapid introduction of valuable selections in the market. However, in vitro cultivation of Helleborus is still very difficult. Thereby the development of reliable in vitro propagation procedures is crucial for future production systems. Axillary buds cultured on agar-solidified Murashige and Skoog medium supplemented with 1 mg/L benzyladenine, 0.1 mg/L β-naphthoxyacetic acid, and 2 mg/L isopentenyl adenine develop shoots after 16 weeks of culture under 16 h light regime, 50-60 μmol/s/m(2), and 19 ± 1°C. The multiplication rate ranges from 1.4 to 2.1. However, the genotype and the number of subcultures affect the efficiency of the micropropagation process. The rooting of shoots is about 80% in solidified MS medium containing 1 mg/L 1-naphthaleneacetic acid and 3 mg/L indole-3-butyric acid. The described protocol provides information which can contribute to the commercial production of Helleborus plants.

Micropropagation in vitro de la variété locale « Aloga » du bananier ...

African Journals Online (AJOL)

Leur transfert en plein champ a permis d'avoir des plantes avec une taille moyenne d'environ 20 cm, un diamètre à la base de la tige avoisinant 10 cm et un nombre de feuilles vertes de l'ordre de 6 après 3 à 4 mois de culture en plein champ. Cette étude ouvre la voie au développement de la micropropagation du plantain ...

Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

OpenAIRE

Silva,Aurilena de Aviz; Carvalho,Almy Junior Cordeiro de; Freitas,Flávia Paiva de; Pessanha,Patrícia Gomes de Oliveira; Santos,Paulo Cesar dos; Silva,Mírian Peixoto Soares da; Vasconcelos,Tábatha de Souza; Olivares,Fábio Lopes

2016-01-01

ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholder...

Micropropagation of Cyclopia genistoides, an endemic South African plant of economic importance.

Science.gov (United States)

Kokotkiewicz, Adam; Luczkiewicz, Maria; Hering, Anna; Ochocka, Renata; Gorynski, Krzysztof; Bucinski, Adam; Sowinski, Pawel

2012-01-01

An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 microM 6-(gamma,gamma-dimethylallylamino)purine (2iP) and 1.0 microM thidiazuron (TDZ). Maximum shoot multiplication rate [(8.2 +/- 1.3) microshoots/explant)] was observed on this medium composition. Prior to rooting, the multiplied shoots were elongated for 60 days (two 30-days passages) on SH medium with one-half sucrose concentration, supplemented with 4.92 microM indole-3-butyric acid (IBA). The rooting of explants was only possible in the case of the elongated shoots. The highest root induction rate (54.8%) was achieved on solid SH medium with one-half sucrose and one-half potassium nitrate and ammonium nitrate concentration, respectively, supplemented with 28.54 microM indole-3-acetic acid (IAA) and 260.25 microM citric acid. The plantlets were acclimatized for 30 days in the glasshouse, with the use of peat/gravel/perlite substrate (1:1:1). The highest acclimatization rate (80%) was obtained for explants rooted with the use of IAA-supplemented medium. The phytochemical profile of the regenerated plants was similar to that of the reference intact plant material. HPLC analyses showed that C. genistoides plantlets obtained by the micropropagation procedure kept the ability to produce xanthones (mangiferin and isomangiferin) and the flavanone hesperidin, characteristic of wild-growing shrubs.

Research Note on the Energy Infrastructure Attack Database (EIAD

Directory of Open Access Journals (Sweden)

Jennifer Giroux

2013-12-01

Full Text Available The January 2013 attack on the In Amenas natural gas facility drew international attention. However this attack is part of a portrait of energy infrastructure targeting by non-state actors that spans the globe. Data drawn from the Energy Infrastructure Attack Database (EIAD shows that in the last decade there were, on average, nearly 400 annual attacks carried out by armed non-state actors on energy infrastructure worldwide, a figure that was well under 200 prior to 1999. This data reveals a global picture whereby violent non-state actors target energy infrastructures to air grievances, communicate to governments, impact state economic interests, or capture revenue in the form of hijacking, kidnapping ransoms, theft. And, for politically motivated groups, such as those engaged in insurgencies, attacking industry assets garners media coverage serving as a facilitator for international attention. This research note will introduce EIAD and position its utility within various research areas where the targeting of energy infrastructure, or more broadly energy infrastructure vulnerability, has been addressed, either directly or indirectly. We also provide a snapshot of the initial analysis of the data between 1980-2011, noting specific temporal and spatial trends, and then conclude with a brief discussion on the contribution of EIAD, highlighting future research trajectories. 

Essential oil obtained from micropropagated lavender, its effect on HSF cells and application in cosmetic emulsion as a natural protective substance.

Science.gov (United States)

Andrys, D; Adaszyńska-Skwirzyńska, M; Kulpa, D

2018-04-01

The aim of the study was to determine the influence of the essential oils isolated from the field - grown and micropropagated in vitro narrow - leaved lavender of the 'Munstead' cultivar, on human skin cells, and their capability to synthesise procollagen. The amount of procollagen type I produced by fibroblast cells was determined using ELISA kit. Essential oil isolated from micropropagated lavender was further used as a protective ingredient against the development of microorganisms in O/W cosmetic emulsion. The presented results demonstrate that the use of 0.01, 0.001 and 0.0001% essential oils isolated from in vitro plants stimulate HSF cells to the production of procollagen. It was further performed that the tested essential oil used in the concentration of 0.1% in a cosmetic emulsion is characterised by preservative effect for cosmetic preparations for the period of 3 months.

Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.).

Science.gov (United States)

Quiroz, Karla A; Berríos, Miguel; Carrasco, Basilio; Retamales, Jorge B; Caligari, Peter D S; García-Gonzáles, Rolando

2017-06-02

Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3-6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.

Micropropagation of Rubus and Ribes spp.

Science.gov (United States)

Dziedzic, Ewa; Jagła, Joanna

2013-01-01

Micropropagation is the most appropriate method for large-scale production of Rubus and Ribes spp. The proliferation rate of Rubus spp. differs in shoot tips and nodal segments. The culture media used for raspberry and blackberry propagation are MS-based supplemented with different combination and ratio of plant growth regulators, depending on the stage of culture. The initiation medium containing 0.4 mg L(-1) BA and 0.1 mg L(-1) IBA is used to stabilize shoot cultures. In multiplication media, concentration of cytokinin is doubled. In vitro rooting of shoots is achieved on media supplemented with 1.0 mg L(-1) IBA. Ribes spp. cultures are initiated from shoot tips, meristem, or dormant buds on MS medium supplemented with 2.0 mg L(-1) BA, 0.5 mg L(-1) IBA, and 0.1 mg L(-1) GA(3.) After stabilization of shoot cultures in 3-4-week time, shoot multiplication is carried out on MS medium containing 1.0 mg L(-1) BA and 0.1 mg L(-1) IBA. Shoots 2 cm long are cultured to rooting on a medium amended with 2.0 mg L(-1) IBA and 5.0 mg L(-1) IAA. Rooted plantlets are transferred to universal peat substrate and acclimatized in the greenhouse.

Research Note On New Limits of the Coefficient of Gravitation ...

Indian Academy of Sciences (India)

Research Note. On New Limits of the Coefficient of Gravitation Shielding. Michele Caputo. Physics Department, University La Sapienza, Piazzale A. Moro 2, 00185 Roma, Italy. and. Department of Geology and Geophysics, Texas A&M University, College Station TX, 77843,. USA. e-mail: mcaputo@g24ux.phys.uniroma1.it.

In vitro propagation of silybum marianum (l.) gaertn. and genetic fidelity assessment of micropropagated plants

International Nuclear Information System (INIS)

Lv, Y.W.; Wamg, R.J.; Yang, Z.S.; Wang, Y.J.

2017-01-01

Silybum marianum (milk thistle), an annual and biennial herbaceous plant, has been used to treat liver disease for 2000 years. An efficient system for micropropagation from leaf explants of wild-grown S. marianum was successfully established and the genetic fidelity of micropropagated plants was assessed using PCR-based random amplified polymorphic DNA (RAPD). The highest frequency (98.9%) of callus induction was obtained from explants cultivated for 4 weeks on Murashige and Skoog (MS) medium supplemented with 1.5 mg l/sup -1/N6-Benzylaminopurine (BA) and 2.0 mg l/sup -1/ alpha-Naphthaleneacetic acid (NAA). The optimal medium for shoots organogenesis was MS supplemented with 3.0 mg l/sup -1/ Gibberellic acid (GA/sub 3/) and 1.0 mg l/sup -1/NAA, while shoot proliferation was accomplished in MS supplemented with 1.0 mg l/sup -1/BA and 0.3 mg l/sup -1/NAA. Rooting (94.5%) of these shoots was achieved after 4 weeks on 1/2 MS medium supplemented with 1.0 mg l/sup -1/ IBA. A total of 92% of the plants were surviving 6 weeks after transplantation of plantlets to soil. RAPD analysis revealed that the genetic fidelity between the parent and the In vitro-raised plantlets exhibited 100% similarity. This confirmed the true-to-type nature of the In vitro-raised clones. |(author)

Research Note Identifying key grazing indicators to monitor trends in ...

African Journals Online (AJOL)

Research Note Identifying key grazing indicators to monitor trends in the veld condition of Lambert's Bay Strandveld, South Africa. ... from which a minimum number of species necessary to monitor trends in the condition of the veld were determined, making it user-friendly for land-users, extension officers and others. The key ...

In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia

Directory of Open Access Journals (Sweden)

Ummi Nur Ain Abdul Razak

2014-02-01

Full Text Available Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP and Kinetin (Kn for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA, Indole-3-butyric acid (IBA and 1-Naphthaleneacetic acid (NAA for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants was observed on a Murashige and Skoog (MS medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in plant soil with survival rate of 83.3 %.

A minimal cost micropropagation protocol for Dianthus caryophyllus L.-- a commercially significant venture.

Science.gov (United States)

Pant, Manu

2016-03-01

In tissue culture, high production cost of the products restricts their reach. Though tissue culture is a major strength in floriculture it is marred by pricing issues. Hence, we developed a complete regeneration low cost micropropagation protocol for an economically important floriculture crop, carnation (Dianthus caryophyllus L.). Successful regeneration of carnation from nodal explants on cost-efficient medium indicates that psyllium husk, sugar and RO water can effectively replace the conventional medium comprising agar, sucrose and distilled water. The protocol can contribute to increased carnation production at comparatively reduced cost, and there by encourage wide scale adoption by the common growers.

Inclusive Education's Promises and Trajectories: Critical Notes about Future Research on a Venerable Idea

Science.gov (United States)

Artiles, Alfredo J.; Kozleski, Elizabeth B.

2016-01-01

The purpose of this article is to offer critical notes on inclusive education research in the U.S. We discuss issues germane to conceptual clarity and the ways in which inclusive education interacts with reforms that share equity goals, noting disruptions and unintended consequences that arise at the nexus of these reforms. In addition, we…

Quality of outpatient clinical notes: a stakeholder definition derived through qualitative research

Directory of Open Access Journals (Sweden)

Hanson Janice L

2012-11-01

content. Conclusions Perspectives of these four stakeholder groups provide a comprehensive description of quality in outpatient clinical documentation. The resulting description of characteristics and content necessary for quality notes provides a research-based foundation for assessing the quality of clinical documentation in outpatient health care settings.

Quality of outpatient clinical notes: a stakeholder definition derived through qualitative research.

Science.gov (United States)

Hanson, Janice L; Stephens, Mark B; Pangaro, Louis N; Gimbel, Ronald W

2012-11-19

groups provide a comprehensive description of quality in outpatient clinical documentation. The resulting description of characteristics and content necessary for quality notes provides a research-based foundation for assessing the quality of clinical documentation in outpatient health care settings.

Micropropagation of Prunus species relevant to cherry fruit production.

Science.gov (United States)

Druart, Philippe

2013-01-01

Cherry tree micropropagation is limited to the production of healthy cultivars of Prunus avium and Prunus cerasus, and their rootstocks; mainly the dwarfing ones. By using meristem-tip (0.1 mm long) or healthy shoot tips/nodes, four successive steps are needed to obtain whole plants capable of growing in the nursery: multiplication by axillary branching, shoot elongation, rooting, and plantlet acclimation. Along this process, several parameters have to be adjusted for each phase of the culture, including media composition, environmental culture conditions and plant handling. These parameters vary depending on genotypic response and specific vulnerability to physiological disorders such as hyperhydricity, apex necrosis, unstable propagation, and rooting rates. Based on a 40 year-long experience of study and application of culture conditions to large-scale plant production, this document summarizes the main problems (variability of the propagation rate, hyperhydricity, apex necrosis, plant re-growth) and solutions encountered to solve them, with means validated on many mericlones.

Micropropagation of Vaccinium sp. by in vitro axillary shoot proliferation.

Science.gov (United States)

Litwińczuk, Wojciech

2013-01-01

The Vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (Vaccinium × corymbosum L.), cranberry (Vaccinium macrocarpon Ait.), and lingonberry (Vaccinium vitis-idaea L.). In some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants could be fulfilled only by micropropagation. In principle cultivars are propagated in vitro through similar three-stage method, based on subculture of shoot explants on different culture media supplemented with IAA (0-4 mg/L) and 2iP (5-10 mg/L), and rooting shoots in vivo. The obtained plantlets are transferred to peat substrate and grown in the glasshouse until the end of growing period. The development of adventitious shoots should be monitored and controlled during in vitro stages. Many clones have specific requirements for growing conditions and/or are recalcitrant.

Research Note: Helping Students Market Themselves with "The Power of Who!"

Science.gov (United States)

Wysong, Scott; Munoz, Laura

2017-01-01

While learning is still of paramount importance, today, colleges and universities realize that they need to equip their students with the skills and knowledge to get hired after graduation. Using the book "The Power of Who!," written by experienced executive recruiter Bob Beaudine, this research note looks at what kind of impact the…

Micropropagation of chokeberry by in vitro axillary shoot proliferation.

Science.gov (United States)

Litwińczuk, Wojciech

2013-01-01

The black chokeberry-aronia (Aronia melanocarpa Elliot) is a shrub native to North America although nowadays well known in Eastern Europe. The fruits are regarded as the richest source of antioxidant phytonutrients among fruit crops and vegetables. Chokeberries can be easily propagated by seeds but this method is not recommended. Micropropagation is far more efficient than other conventional cloning methods like layering or softwood cuttings. Aronia clones are propagated in vitro through four- or three-stage method based on subculturing of shoot explants. The double diluted MS or full strength MS medium with elevated 50% Ca(2+) and Mg(2+) content are used in the initiation and proliferation chokeberry in vitro cultures, respectively. They are supplemented with 0.5-1.0 mg LBA, and 0.05 mg LIBA. The double-phase medium is recommended in the last passage before shoot rooting. The regenerated shoots could be rooted both in vitro on double diluted MS with 0.05 mg L(-1) IBA or in vivo in peat and perlite substrate and subsequently grown in the greenhouse.

Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites

Directory of Open Access Journals (Sweden)

Barbara Thiem

2014-01-01

Full Text Available A method was devised for the micropropagation of eight species of the genus Oenothera L. from shoot tips and shoot segments with nodes. Microshoot cultures were obtained from explants on Murashige and Skoog (MS medium enriched with IAA and BA. Numerous shoots developed properly after they had been transferred onto MS medium without BA. When they had rooted under the influence of auxins (IAA or IBA, shoots were transferred to pots and then to the soil, where they matured. In the seeds produced by these plants, the content of fatty acids was determined using the GC method. A preliminary analysis of flavonoid compounds and phenolic acids was made using the 2D TLC method (fingerprinting in microshoots and leaves of soil plants regenerated in vitro.

MixedNotes

DEFF Research Database (Denmark)

Jokela, Tero; Lucero, Andrés

2014-01-01

Affinity Diagramming is a technique to organize and make sense of qualitative data. It is commonly used in Contextual Design and HCI research. However, preparing notes for and building an Affinity Diagram remains a laborious process, with a wide variety of different approaches and practices....... In this paper, we present MixedNotes, a novel technique to prepare physical paper notes for Affinity Diagramming, and a software tool to support this technique. The technique has been tested with large real-life Affinity Diagrams with overall positive results....

Advancing medicine one research note at a time: the educational value in clinical case reports.

Science.gov (United States)

Cabán-Martinez, Alberto J; Beltrán, Wilfredo F García

2012-07-06

A case report--a brief written note that describes unique aspects of a clinical case--provides a significant function in medicine given its rapid, succinct, and educational contributions to scientific literature and clinical practice. Despite the growth of, and emphasis on, randomized clinical trials and evidenced-based medicine, case reports continue to provide novel and exceptional knowledge in medical education. The journal BMC Research Notes introduces a new "case reports" section to provide the busy clinician with a forum in which to document any authentic clinical case that provide educational value to current clinical practice. The aim is for this article type to be reviewed, wherever possible, by specialized Associate Editors for the journal, in order to provide rapid but thorough decision making. New ideas often garnered by and documented in case reports will support the advancement of medical science--one research note at a time.

Revisiting convergence: A research note.

Science.gov (United States)

Clark, Rob

2015-09-01

A number of recent studies show that income inequality is declining between countries. In this research note, I question the significance of this trend by examining the role of initial conditions in producing convergence. An important (but neglected) property of inequality dynamics is the tendency for extreme distributions to become more moderate. When income disparities are large, the subsequent trend is biased toward convergence. Conversely, when initial conditions approach parity, divergence becomes the more likely long-term outcome. I apply this principle to trends in GDP PC across 127 countries during the 1980-2010 period. Using counterfactual analysis, I manipulate the initial level of inequality in GDP PC while holding constant each country's observed growth rate during the sample period. I find that the growth dynamics of GDP PC produce either convergence or divergence based simply on the initial distribution of income. The point of transition occurs at a moderate level of inequality, whether using population weights (Gini=.365) or not (Gini=.377). I conclude that the recent convergence observed in GDP PC is primarily a function of large income gaps between countries and would not have materialized at more moderate levels of initial inequality. By contrast, an examination of the pre-1950 period reveals divergent growth patterns that are not sensitive to initial conditions. Copyright © 2015 Elsevier Inc. All rights reserved.

[Study on Rapid Micropropagation in Vitro Technique of Guangfeng Medicinal Yam (Dioscorea opposita) Plantlets].

Science.gov (United States)

Yin, Ming-Hua; Xu, Zhi-Jian; Zhang, Sheng-qin; Lv, Si-Jie; Zeng, Yan-hong; Fu, You-zhang; Hong, Sen-rong

2015-11-01

In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied. Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected. The best disinfection procedure of stems with a bud of Guangfeng medicinal yam was washed with sterile water for three times after sterilized with 70% alcohol for 20 - 30 s and then washed with sterile water for three times again after sterilized with 0.1% mercuric chloride for 10 - 12 min; The best explants of stems with a bud of Guangfeng medicinal yam was slightly woody and more mature stems witha bud; The best proliferation culture medium of stems with a bud of Guangfeng medicinal yam was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L; The best rooting culture medium of stems with a bud of Guangfeng medicinal yam was MS + NAA 0.5 mg/L; The best culture method of Guangfeng medicinal yam plantlets was liquid culture; The best transplanting matrix of Guangfeng medicinal yam plantlets was the mixture of paddy clay and fine sand (1: 2) or the mixture of perlite and vermiculite (1: 2); The DNA content between Guangfeng medicinal yam plantlets and its potted seedlings had no significant difference. A fast and efficient micropropagation in vitro technological system of stems with a bud of Guangfeng medicinal yam is established, and the flow cytometry detect results also show the genetic stability of Guangfeng medicinal yam plantlets, whose results provide the technical and theoretical basis for the large-scale production of Guangfeng medicinal yam plantlets.

Advancing medicine one research note at a time: the educational value in clinical case reports

Directory of Open Access Journals (Sweden)

Cabán-Martinez Alberto J

2012-07-01

Full Text Available Abstract A case report—a brief written note that describes unique aspects of a clinical case—provides a significant function in medicine given its rapid, succinct, and educational contributions to scientific literature and clinical practice. Despite the growth of, and emphasis on, randomized clinical trials and evidenced-based medicine, case reports continue to provide novel and exceptional knowledge in medical education. The journal BMC Research Notes introduces a new “case reports” section to provide the busy clinician with a forum in which to document any authentic clinical case that provide educational value to current clinical practice. The aim is for this article type to be reviewed, wherever possible, by specialized Associate Editors for the journal, in order to provide rapid but thorough decision making. New ideas often garnered by and documented in case reports will support the advancement of medical science — one research note at a time.

Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’

Directory of Open Access Journals (Sweden)

Kamnoon Kanchanapoom

2010-01-01

Full Text Available A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’ was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

Research Note The reliability of a field test kit for the detection and ...

African Journals Online (AJOL)

Research Note The reliability of a field test kit for the detection and the persistence of ... Open Access DOWNLOAD FULL TEXT ... The objectives were to test a field kit for practicality and reliability, to assess the spread of the bacteria among ...

The influence of anticipated regret on business start-up behaviour: A research note

NARCIS (Netherlands)

Hatak, Isabella; Snellman, Kirsi

This research note aims to develop the emotional underpinning of early-stage entrepreneurship by examining the influence of anticipated regret, a negative emotion, on the transformation of latent entrepreneurs into nascent entrepreneurs. Drawing on regret regulation theory and two waves of survey

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments.

Science.gov (United States)

Silveira, S S; Cordeiro-Silva, R; Degenhardt-Goldbach, J; Quoirin, M

2016-05-03

Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium, at any concentration. Polyvinylpyrrolidone, activated charcoal, cysteine, ascorbic acid or citric acid were added to the culture medium to avoid oxidation. After 30 days of culture, polyvinylpirrolidone and ascorbic acid gave better results, eliminating oxidation in most explants. For shoot multiplication, benzylaminopurine was used in concentrations of 4.4 and 8.8 µM in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively, after 90 days. Indole-3-butyric acid and α-naphthalene acetic acid were used to induce root formation, reaching a maximum rooting rate of 24% with 20µM α-naphthalene acetic acid. For acclimatization. the rooted plants were transferred to Plantmax® substrate and cultured in a greenhouse, reaching 79% of survival after 30 days and 60% after one year.

Researches on Mathematical Relationship of Five Elements of Containing Notes and Fibonacci Sequence Modulo 5

Directory of Open Access Journals (Sweden)

Zhaoxue Chen

2015-01-01

Full Text Available Considering the five periods and six qi’s theory in TCM almost shares a common basis of stem-branch system with the five elements of containing notes, studying the principle or mathematical structure behind the five elements of containing notes can surely bring a novel view for the five periods and six qi’s researches. By analyzing typical mathematical rules included in He tu, Luo shu, and stem-branch theory in TCM as well as the Fibonacci sequence especially widely existent in the biological world, novel researches are performed on mathematical relationship between the five elements of containing notes and the Fibonacci sequence modulo 5. Enlightened by elementary Yin or Yang number grouping principle of He tu, Luo shu, the 12534 and 31542 key number series of Fibonacci sequence modulo 5 are obtained. And three new arrangements about the five elements of containing notes are then introduced, which have shown close relationship with the two obtained key subsequences of the Fibonacci sequence modulo 5. The novel discovery is quite helpful to recover the scientific secret of the five periods and six qi’s theory in TCM as well as that of whole traditional Chinese culture system, but more data is needed to elucidate the TCM theory further.

Micropropagation and Biomass Production of True-to-Type Stevia rebaudiana Bertoni.

Science.gov (United States)

Modi, Arpan R; Sharma, Vikas; Patil, Ghanshyam; Singh, Amritpal S; Subhash, N; Kumar, Nitish

2016-01-01

Here we describe an efficient micropropagation protocol for Stevia rebaudiana Bertoni. We present experiments carried out to optimize the suitable media for in vitro shoot multiplication and root induction and to study the effect of culture vessel on shoot multiplication. Among all different media tested for in vitro shoot multiplication, hormone-free liquid medium is most suitable. The highest number of nodes per shoot (5.4) and length of shoot (4.76 cm) at 4 weeks after subculturing are observed when single node explants are placed on modified MS medium supplemented with 1 % sucrose and 0.7 % agar. The highest response of multiplication rate (9.56) is observed on half strength of macroelement of MS with full strength of microelement of MS and 170 mg/l KH2PO4, and 185 mg/l MgSO4 in plastic growth container. Further, RAPD marker analysis of in vitro-raised plants maintained their clonal fidelity and true-to-type without showing any somaclonal variation.

Control of media browning in micropropagation of guava (Psidium guajava L.)

International Nuclear Information System (INIS)

Ahmad, I.; Nafees, M.; Ashraf, I.

2016-01-01

Guava (Psidium guajava L.) is a highly valuable fruit of the tropical regions of the world. This species faces browning or blackening of culture medium during In vitro culture due to leaching of phenolic, microbial contagion and tissue recalcitrance. A study therefore designed to evaluate the effects of antioxidants in reduction of phenolic exudation which hampers In vitro regeneration. The nodal explants of the plant were cultured on MS media after pre-soaking in antioxidant solutions of citric acid, ascorbic acid, poly vinyl pyrrolidine (PVP) and charcoal. After culturing explants, the amount of phenolic exude was determined periodically on spectrophotometer at 750 nm absorbance. Phenolic exudation from guava was significantly reduced in nodes treated with charcoal as compared to control and rest of the treatments. Moreover, guava nodes survival percentage was also significantly increased in charcoal treated nodes. It is concluded that pre-soaking in different antioxidants significantly reduced the media browning and thus micro-propagation of guava could be achieved on commercial basis. (author)

Certain growth related attributes of micropropagated banana under different salinity levels

International Nuclear Information System (INIS)

Haq, I.U.; Soomro, F.

2011-01-01

The effect of salinity (NaCl) was assessed on banana (Musa spp.) cv., Sindhri Banana (Basrai) propagating plantlets in aseptic condition. Four different NaCl levels [0 (control) 50, 100 and 150 mM] were maintained at shoot multiplication stage for 6-weeks. Salinity reduced the number of plantlets per explants and plant biomass significantly. A proportional relationship was observed for Na/sup +/ and Cl/sub -/ but K/sup +/, Ca/sup 2+/and NO/sub 3/ were observed to be inversely proportioned with NaCl stress. Similarly, total proteins as well as carbohydrate contents were decreased significantly. Increasing mode of secondary metabolites (proline, betaine contents and reducing sugars) were showing a negative relationship of saline stress with plant micro-propagation efficiency. Among photosynthetic pigments, total carotenoids were increased while chlorophyll contents (Chl a and b) decreased. Similarly, nitrate reductase activity also reduced. Overall, vegetative propagation of banana was affected significantly by NaCl stress under in-vitro conditions. (author)

Micropropagation of the new apple rootstock ‘G. 814’

Directory of Open Access Journals (Sweden)

Aline Meneguzzi

Full Text Available ABSTRACT: International breeding programs launched new genetic material of apple rootstocks that in addition to precocity and great yield are resistant to major diseases and soil pests encountered in the largest apple producing regions in Brazil. Given this, there is a necessity for vegetative propagation of these materials for study and possible replacement of existing rootstocks. The objective was to adapt a micropropagation protocol for new apple rootstock ‘G. 814’. In the multiplication phase were evaluated BAP concentrations: 0; 0.5; 1; 2 and 4mg L-1 and in the rooting phase were evaluated IBA concentrations: 0; 0.25; 0.50; 1; 1.5 and 2.5mg L-1. These new results demonstrated that this new rootstock selection can be propagated with this tissue culture adapted protocol. For the successful in vitro propagation of apple rootstock ‘G. 814’ it is indicated the use of 1mg L-1 BAP at multiplication phase and 1.5mg L-1 IBA at rooting phase.

Note-Taking Techniques for Students with Disabilities: A Systematic Review of the Research

Science.gov (United States)

Boyle, Joseph R.; Rivera, Tina Z.

2012-01-01

This article provides a synthesis of note-taking research among students with learning disabilities or other high-incidence disabilities. A search of the professional literature between 1980 and 2010 yielded nine intervention studies, which were evaluated either in terms of effect size or percentage of nonoverlapping data. The studies examined…

Micropropagation of Dianthus deltoides L. through shoot tip and nodal cuttings culture

Directory of Open Access Journals (Sweden)

Marković Marija

2013-01-01

Full Text Available Micropropagation (shoot tip and nodal cuttings culture was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L. in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP and naphthaleneacetic acid (NAA. The highest multiplication rate was achieved on a medium containing 0.1 mgL-1 of BAP and 0.1 mgL-1 of NAA. The rooting was successful on a hormone-free medium (100%, and the highest percentage of microplant acclimatization (97% was recorded in a 4: 1 mixture of peat and sand. [Projekat Ministarstva nauke Republike Srbije, br. TR 31041: Establishment of wood plantations intended for a forestation of Serbia

Effects of growth regulators and activated charcoal for the micropropagation improvement of Dendrobium friedericksianum Rchb.f.

Directory of Open Access Journals (Sweden)

Prasertsongskun, S. and

2004-09-01

Full Text Available The effects of different concentrations of growth regulators and activated charcoal on micropropagation were studied. Seedlings of orchid, Dendrobium friedericksianum Rchb.f, cultured on Vacin and Went medium containing 1.0 mg/l α-naphthaleneacetic acid (NAA and 1.0 mg/l kinetin (KN significantly yielded per cultured plant, the highest average number of leaves (4.40, roots (8.30, shoot length (1.67 centimeters and fresh weight (0.23 grams. Particularly when activated charcoal was added (0.3% w/v the average number of leaves, roots, shoot length and fresh weight per cultured plant increased. Percentage survival of the plantlet after 21 days of transplantation was 85%.

Notes on Contributors

African Journals Online (AJOL)

NOTES ON CONTRIBUTORS. Samuel AMOAKO, Associate Researcher, South African Research Unit in Social Change. Contact Details: C/o Lucinda Bercony, Humanities Research Village (House No. 3). University of Johannesburg, P. O. Box 524. Bunting Road Campus, Auckland Park, 2006.

Financial accounting: an epistemological research note

Directory of Open Access Journals (Sweden)

Eduardo Schiehll

2007-12-01

Full Text Available This research note is the result of the authors' reflections on epistemological issues in respect to the financial accounting field. From an epistemological perspective, this document attempts to trace the philosophical, historical, sociological, and discursive research perspectives that have guided academic research in the field of financial accounting. In order to do so, this document explores the distinctions and connections between accounting theory and accounting practice, which we believe is the first step towards understanding accounting as a scientific discipline. We analyze the theories underpinning financial accounting research, discussing its purposes, historic evolution, and scientific methods used. This document also discuss the sociological and discursive contexts of financial accounting in order to demonstrate that, like every other social science, accounting research is based upon assumptions about the nature of it players, or social networks. This document does not have the pretension to cover or close the discussion about all the pitfalls of this complex topic. In this sense, we try to document our analysis and draw some arguments in order to offer evidence for further discussion.Este ensaio teórico é resultado de reflexões dos autores acerca de questões epistemológicas sobre a contabilidade financeira. Através de uma perspectiva epistemológica, este trabalho busca traçar perspectivas filosóficas, históricas, sociológicas e discursivas que guiaram a pesquisa acadêmica na área da contabilidade financeira. Para isso, exploram-se as similaridades e diferenças entre a teoria e a prática contábil, pois esse é o primeiro para a compreensão da contabilidade como disciplina científica. Analisam-se as teorias que suportam a pesquisa em contabilidade financeira, discutindo seu propósito, evolução histórica e métodos científicos utilizados. O presente trabalho também discute os contextos sociológicos e

Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.

Science.gov (United States)

Vilanova Neta, Jaci Lima; da Silva Lédo, Ana; Lima, Aloisio André Bonfim; Santana, José Carlos Curvelo; Leite, Nadjma Souza; Ruzene, Denise Santos; Silva, Daniel Pereira; de Souza, Roberto Rodrigues

2012-09-01

The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.

Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

Science.gov (United States)

Watad, A A; Kochba, M; Nissim, A; Gaba, V

1995-03-01

An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die.

To inhabit research-creation labs notes from the experience

Directory of Open Access Journals (Sweden)

Mónica Marcell Romero Sánchez

2012-06-01

Full Text Available Current arts practices have exceeded their limits to the point that a creator is considered to be a deep thinker of her/himself and of the actions that she/ he accomplishes in specific contexts. Therefore, arts practices are formed by the tradition of their disciplines and their numerous transformations, as well as by a high reflexive component around its social function of integrating the community. Based on such a premise, I will deal with some considerations about the national program of visual arts education in the informal field: the research-creation labs (implemented in 2004 under the tutelage of the Ministry of Culture – Arts Department that have influenced the way of teaching arts in our country and their ways of passing around and articulating with the Regional and National Artists Rooms. The shared reflections are part of the research project: ‘Among institutional discourses and micronarratives. Notes for the construction of a critical look at the visual arts research-creation labs’, which is in process and has its interest focused on discussing the arts practices with respect to the pedagogical practices in the institutional framework they are part of and on demonstrating in this way their tensions.

Evaluation of genetic variability in micropropagated propagules of ornamental pineapple [Ananas comosus var. bracteatus (Lindley) Coppens and Leal] using RAPD markers.

Science.gov (United States)

Santos, M D M; Buso, G C S; Torres, A C

2008-10-21

The objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. The basal culture medium consisted of MS salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/L. The addition of BAP influenced the occurrence of genetic variation revealed using random amplified polymorphic DNA (RAPD) markers. Of a total of 520 primers tested, 44 were selected and amplified; 402 monomorphic bands (97.2%) and 18 polymorphic bands (2.8%) resulted among regenerated plantlets. The polymorphic fragments were produced by 12 primers (OPA-01, OPA-20, OPB-01, OPB-19, OPC-19, OPF-13, OPL-17, OPM-13, OPP-16, OPT-07, OPV-19, and OPX-03). Among the primers that identified polymorphism, OPA-01, OPA-20, OPB-19, OPC-19, OPL-17, OPP-16, and OPX-3 each showed, one polymorphic band and OPF-13 amplified a maximum of three bands. In this study, the RAPD technique was effective in showing the occurrence of somaclonal variations that occur during the micropropagation process of ornamental pineapple cultivation in BAP-supplemented medium, and it is possible to detect the presence of genetic variation in early stages of plant development.

Effects of Note-Taking Instruction and Note-Taking Languages on College EFL Students' Listening Comprehension

Science.gov (United States)

Tsai-Fu, Tsai; Wu, Yongan

2010-01-01

Background: The effect of note-taking has been well-recognized by EFL educators. However, little empirical research has been done to investigate combined effects of note-taking instruction and note-taking language (whether in L1 or L2) in an acquisition-poor environment, where English is used as an instructional language yet the audience is…

Micropropagation and subsequent enrichment of carotenoids, fatty acids and tocopherol contents in Sedum dasyphyllum L

Science.gov (United States)

Park, Han Yong; Saini, Ramesh Kumar; Gopal, Judy; Keum, Young-Soo; Kim, Doo Hwan; Lee, Onew; Sivanesan, Iyyakkannu

2017-10-01

A promising micropropagation protocol has been systematically established and demonstrated for the enhanced production of carotenoids, tocopherol and fatty acids in shoot tissues of Sedum dasyphyllum. Shoot tip explants were grown on Murashige and Skoog (MS) medium. Different concentrations of N6-benzyladenine (BA) or thidiazuron (TDZ) alone or in combination with α-naphthaleneacetic acid (NAA) were tested in order to stimulate multiple shoot production. Ideal shoot induction (100%) and maximized shoot numbers (36.4) were obtained on explants cultured on media incorporated with 2 µM BA and 1 µM NAA combinations. The in vitro-developed shoots rooted best on half-strength MS media incorporated with 2 µM indole 3-butyric acid. Plantlets were effectively acclimatized in the greenhouse with 100% survival rate. The composition and contents of bioactive compounds such as carotenoids, tocopherol and fatty acids in shoot tissues of S. dasyphyllum were investigated using HPLC and GC-MS. The most abundant carotenoid in the shoot tissue was all-E-lutein (40.3-70.5 µg g-1 FW) followed by 9'-Z-neoxanthin (5.3-9.9 µg g-1 FW), all-E-violaxanthin (4.4-8.2 µg g-1 FW), and all-E-β-carotene (1.6-3.6 µg g-1 FW). The α-tocopherol contents of in vitro-raised shoots was 6.5-fold higher than shoots of greenhouse-grown plants. The primary fatty acids found in shoot tissues were α-linolenic acid (32.0-39.3%), linoleic acid (27.4-38.2%), palmitic acid (13.3-15.5%) and stearic acid (5.2-12.2%). In all, summarizing the findings, the micropropagated S. dasyphyllum showed significant enrichment of valuable bioactive carotenoids (92.3 µg g-1 FW), tocopherols (14.6 µg g-1 FW) and α-linolenic acid (39.3%) compared to their greenhouse counterparts. The protocol demonstrated here could be applied for the mass propagation and production of enhanced bioactive compounds from S. dasyphyllum with credibility.

Micropropagation and validation of genetic and biochemical fidelity amongst regenerants of Cassia angustifolia Vahl employing RAPD marker and HPLC.

Science.gov (United States)

Chetri, Siva K; Sardar, Pratima Rani; Agrawal, Veena

2014-10-01

In vitro protocol has been established for clonal propagation of Cassia angustifolia Vahl which is an important source of anticancerous bioactive compounds, sennoside A and B. Nodal explants excised from field raised elite plant (showing optimum level of sennoside A and B) of C. angustifolia when reared on Murashige and Skoog's medium augmented with different cytokinins, viz. N(6)-benzyladenine (BA), N(6)-(2-isopentenyl) adenine (2iP) and 6-furfuryl aminopurine (Kn) differentiated multiple shoots in their axils. Of the three cytokinins, BA at 5 μM proved optimum for differentiating multiple shoots in 95 % cultures with an average of 9.14 shoots per explant within 8 weeks of culture. Nearly, 95 % of the excised in vitro shoots rooted on half strength MS medium supplemented with 10 μM indole-3-butyric acid (IBA). The phenotypically similar micropropagated plants were evaluated for their genetic fidelity employing random amplified polymorphic DNA (RAPD) markers. Eleven individuals, randomly chosen amongst a population of 120 regenerants were compared with the donor plant. A total of 36 scorable bands, ranging in size from 100 to 1,000 bp were generated amongst them by the RAPD primers. All banding profiles from micropropagated plants were monomorphic and similar to those of mother plant proving their true to the type nature. Besides, high performance liquid chromatography evaluation of the sennoside A and B content amongst leaves of the mature regenerants and the elite mother plant too revealed consistency in their content.

Micropropagation of Alibertia edulis Rich

Directory of Open Access Journals (Sweden)

Fernanda Augusta Boato da Silva

2008-12-01

Full Text Available The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA alone or combined with indole-3-butyric acid (IBA was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures also had a positive effect on both sprouting and shoot elongation. Activated charcoal was essential for rooting, and all rooted plants were able to acclimatize.Foi desenvolvido um protocolo de micropropagação para A. edulis, uma espécie arbórea frutífera e medicinal presente no Cerrado brasileiro. Sementes de A. edulis foram esterilizadas e germinadas em ágar-água. Explantes de 15 mm foram retirados das plântulas e cultivados por 50 dias em meio de multiplicação e três subculturas. A citocinina benzilalanina (BA, sozinha ou em combinação com ácido indol butírico (IBA foram adicionados em no meio ¼ MS. Os tratamentos contendo combinações de BA-IBA induziram uma maior proliferação de brotos que aqueles somente com BA, e a concentração ótima desses reguladores de crescimento foi de 0.5 mg.L-1 para cada. Os brotos de A. edulis facilmente se alongaram e a presença de reguladores de crescimento no meio de cultura teve efeito positivo sobre o alongamento. Da mesma forma, as consecutivas subculturas tiveram um efeito positivo tanto sobre a brotação quanto no alongamento. Carvão ativado foi essencial para o enraizamento dos ramos, e todas as plantas foram aclimatadas com sucesso.

Micropropagation of onion (Allium cepa L.) from immature inflorescences.

Science.gov (United States)

Marinangeli, Pablo

2013-01-01

In vitro plant production by direct organogenesis from immature flower heads is an ideal approach for clonal propagation of onions (Allium cepa L.). This technique ensures genetic stability, high propagation rate, and maintains donor plant of explants with an advantage over other means of in vitro regeneration. Onion micropropagation is usually applied in breeding programs, maintenance, and multiplication of cytoplasmic-male sterile lines for hybrid production, germplasm conservation, and as a tool for the application of other biotechnologies. For in vitro culture, mature onion bulbs are induced to reproductive phase by vernalization and forced to inflorescence initiation. Immature umbels are dissected from bulbs or cut directly when they appear from the pseudostem among the leaves. Disinfected inflorescences are cultivated in BDS basal medium supplemented with 30 g/L sucrose, 0.1 mg/L naphthalene acetic acid, 1 mg/L N (6)-benzyladenine, and 8 g/L agar, pH 5.5, under 16 h photoperiod white fluorescent light (PPD: 50-70 μmol/m(2)s) for 35 days. The regenerated shoot clumps are divided and subculture under the same conditions. For bulbification phase, the individual shoots are cultured in BDS basal medium containing 90 g/L sucrose, without plant growth regulators, pH 5.5, under 16 h photoperiod. Microbulbs can be directly cultivated ex vitro without acclimation.

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Directory of Open Access Journals (Sweden)

Daniele Brandstetter Rodrigues

2016-10-01

Full Text Available An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated- various concentrations of 6-benzylaminopurine (BAP (0.0, 1.0, 2.0, and 3.0 mg L-1 and substrates (agar, vermiculite, and coconut fiber added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA (0.0, 0.5, 1.0, and 1.5 mg L-1 were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L-1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture.

Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

Directory of Open Access Journals (Sweden)

Marković Marija

2015-01-01

Full Text Available The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%, but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%. However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution. [Projekat Ministarstva nauke Republike Srbije, br. 43007

Micropropagation of Salvia wagneriana Polak and hairy root cultures with rosmarinic acid production.

Science.gov (United States)

Ruffoni, Barbara; Bertoli, Alessandra; Pistelli, Laura; Pistelli, Luisa

2016-01-04

Salvia wagneriana Polak is a tropical species native to Central America, well adapted to grow in the Mediterranean basin for garden decoration. Micropropagation has been assessed from axillary shoots of adult plants using a Murashige and Skoog basal medium, with the addition of 1.33-μM 6-benzylaminopurine for shoot proliferation; the subsequent rooting phase occurred in plant growth regulator-free medium. The plants were successfully acclimatised with high survival frequency. Hairy roots were induced after co-cultivation of leaf lamina and petiole fragments with Agrobacterium rhizogenes and confirmed by PCR. The establishment and proliferation of the selected HRD3 line were obtained in hormone-free liquid medium and the production of rosmarinic acid (RA) was evaluated after elicitation. The analysis of RA was performed by LC-ESI-DAD-MS in the hydroalcoholic extracts. The addition of casein hydrolysate increased the RA production, whereas no enrichment was observed after the elicitation with jasmonic acid.

Zearalenone Uptake and Biotransformation in Micropropagated Triticum durum Desf. Plants: A Xenobolomic Approach.

Science.gov (United States)

Rolli, Enrico; Righetti, Laura; Galaverna, Gianni; Suman, Michele; Dall'Asta, Chiara; Bruni, Renato

2018-02-14

A model was set up to elucidate the uptake, translocation, and metabolic fate of zearalenone (ZEN) in durum wheat. After treatment with ZEN, roots and shoots were profiled with LC-HRMS. A comprehensive description of in planta ZEN biotransformation and a biotechnological evaluation of the model were obtained. Up to 200 μg ZEN were removed by each plantlet after 14 days. Most ZEN and its masked forms were retained in roots, while minimal amounts were detected in leaves. Sixty-two chromatographic peaks were obtained, resulting in 7 putative phase I and 18 putative phase II metabolites. ZEN16Glc and ZEN14Glc were most abundant in roots, sulfo-conjugates and zearalenol derivatives were unable to gain systemic distribution, while distinct isomers of malonyl conjugates were found in leaves and roots. This study underlines the potential ZEN occurrence in plants without an ongoing Fusarium infection. Micropropagation may represent a tool to investigate the interplay between mycotoxins and wheat.

Micropropagation of Phalaenopsis orchids via protocorms and protocorm-like bodies.

Science.gov (United States)

Paek, Kee Yoeup; Hahn, Eun Joo; Park, So Young

2011-01-01

Phalaenopsis orchids have high economic value in the floriculture industry. Hybridization or cross-pollination in the breeding program have proven to be very reliable techniques for the production of a wide range of successful cultivars with attractive combinations of spray length, bud number, flower color and type, fragrance, seasonality, and compactness. In vitro propagation makes it possible to clonally mass propagate hybrids of commercial value and conserved species. However, in vitro culture technologies are still a challenge because of the slow growth of plantlets, low multiplication rate, poor rooting, and somaclonal variation. Although seed-raised plants can be used for conservation and breeding for the selection of superior features, genetic characteristics including seasonality, inflorescence, flower color, and type are not uniform. In this regard, micropropagation through protocorm-like bodies obtained from germinating embryos and somatic tissues is an important strategy in obtaining genetically stable plants and the improvement of quality. However, not all genotypes of Phalaenopsis respond to the same protocol under the same culture conditions and often result in the development of undesirable characteristics. In this chapter, plantlet production in Phalaenopsis orchids via the culture of protocorms from seeds and protocorm-like bodies from leaf sections and root tips are detailed.

Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress.

Science.gov (United States)

Saher, Shady; Piqueras, Abel; Hellin, Eladio; Olmos, Enrique

2004-01-01

The physiology of hyperhydricity in relation to oxidative stress, mineral nutrients, antioxidant enzymes and ethylene has been studied in three micropropagated carnation cultivars under experimentally induced hyperhydricity. A marked increase in Fe content in comparison with normal tissues was observed in the hyperhydric tissues from the three cultivars. The levels of ethylene, solute leakage and malondialdehyde content were also significantly higher in the hyperhydric tissues. In relation to the time course of H(2)O(2) production measured by fluorescence quenching, a similar trend could be observed for the three cultivars, with a clear increase in the generation of hydrogen peroxide in hyperhydric tissues. The activities of all the antioxidative enzymes studied, except lipoxygenase, were higher in the hyperhydric shoots. Phenylalanine ammonia-lyase (PAL) showed a significant decrease in activity in the hyperhydric tissues in comparison with the controls for the three cultivars. Soluble guaiacol peroxidase had a strong increase in activity in hyperhydric shoots of the three cultivars. These results provide, for the first time, direct evidence of H(2)O(2) generation in hyperhydric tissues, characterize the response of the antioxidant system to an oxidative stress during hyperhydricity in carnation leaves and point to the accumulation of toxic forms of oxygen as the inducer of some of the abnormalities observed.

Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae).

Science.gov (United States)

Braga, Virgínia F; Mendes, Giselle C; Oliveira, Raphael T R; Soares, Carla Q G; Resende, Cristiano F; Pinto, Leandro C; Santana, Reinaldo de; Viccini, Lyderson F; Raposo, Nádia R B; Peixoto, Paulo H P

2012-03-01

This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC(50) = 169.3 µg mL(-1). HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg(-1), which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis.

Review by a local medical research ethics committee of the conduct of approved research projects, by examination of patients' case notes, consent forms, and research records and by interview.

OpenAIRE

Smith, T.; Moore, E. J.; Tunstall-Pedoe, H.

1997-01-01

OBJECTIVE: To monitor the conduct of medical research projects that have already been approved by the local medical research ethics committee. DESIGN: Follow up study of ethically approved studies (randomly selected from all the studies approved in the previous year) by examination of patients' case notes, consent forms, and research records and by interview of the researchers at their workplace. SETTING: Tayside, Scotland (mixed rural and urban population). SUBJECTS: 30 research projects app...

Micropropagation of Capparis decidua (Forsk.) Edgew. - a tree of arid horticulture.

Science.gov (United States)

Deora, N S; Shekhawat, N S

1995-12-01

A method for micropropagation of mature trees of Capparis decidua was developed. Multiple shoots were obtained from nodal explants on Murashige and Skoog's (1962) medium+0.1mgl(-1) NAA+5.0mgl(-1)BAP+additives (50mgl(-1) ascorbic acid and25 mgl(-1) each of adenine sulphate, L-arginine and citric acid) at 28 ± 2°C, 12 h/dphotoperiod and 35-40 μmol m(-2)s(-1) photon flux density. The shoots were multiplied by (i) subculture of nodal shoot segments onto MS +0.1 mgl(--1) IAA+1.0mgl(-1) BAPH+additives, and (ii) repeated transfer of original explant onto MS+ 0.1mgl(-1) IAA+mg l(-1) BAP+additives, at intervals of 3 weeks. Sixty to 70% of the shoots rooted when pulse treated with 100 mg l(-1) IBA in half strength MS liquid medium for 4h, and then transferred onto hormone-free half-strength agar-gelled MS basal saltmedium. Incubation in dark at 33 ± 2°C for 6d favoured root induction. In vitro hardened plants were transferred to pots.

Tissue culture and micropropagation for forest biomass production

Energy Technology Data Exchange (ETDEWEB)

Mason, E.; Maine, F.W.

1984-09-01

An increase in forest production will be necessary in the future when wood becomes a major renewable source of energy and chemicals along with its traditional role of fibre source. This increase could eventually by achieved be proper selection and breeding of trees. Clonal forestry by vegetative propagation of cuttings is becoming a viable alternative to a seedling-based forestry with many advantages, and cutting could be used to quickly propagate large numbers of clones of control-pollinated seedlings. Most forest trees are propagated sexually and seed orchards were started in the US and Canada in the last 40-50 years for breeding purposes. Forests could ultimately be established with improved seedlings instead of from seed with unknown genetic potential, or by natural regeneration. Micropropagation is the term used to refer to the propagation of plants raised by tissue culture methods rather than from seeds or cuttings. Many clonal plantlets could be regenerated asexually in the laboratory and eventually transplanted to permanent sites. In addition the technology could be developed to produce new variants from somatic cells. Tissue culture is a technique which may be useful for plant propagation where conventional methods are inadequate or unsuitable. However, traditional studies of field planting observed over long periods of time would still be necessary. This document has the object of informing those who may wish to know more about these techniques in relation to practical application, and require a general overview rather than experimental details, which are given in an annotated bilbiography. 274 refs., 2 figs., 1 tab.

In Vitro micropropagation of Lawsonia inermis (Lythraceae

Directory of Open Access Journals (Sweden)

G.R. Rout

2001-12-01

Full Text Available A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962 medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA, 0.25 mg/l Kinetin (Kn, 0.5 mg/l ascorbic acid and 3% (w/v sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14h photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soilSe desarrolo un protocolo exitoso para la propagación en masa de Lawsonia inermis Linn., una planta medicinal importante. Se indujeron múltiples tallos en meristamos apicales y auxiliares derivados de explantes maduros de L. inermis en medio de Murashige y Skoog (1962 suplementado con 0.25 mg/l 6-benzylaminopurina (BA, 0.25 mg/l quinetina (Kn, 0.5 mg/l ácido ascórbico y 3 % (w/v sucrosa. La tasa de multiplicación fue más alta cuando los cultivos fueron incubados bajo luz continua que bajo el fotoperíodo de 14hr. El enraizamiento se logró al tranferir los microtallos al medio basal, semi-sólido MS suplementado con 0.25 mg/l de ácido indol-3-butiríco (IBA luego de 10 días de cultivo. Las plántulas micropropagadas fueron aclimatizadas y cultivadas con éxito en el suelo

Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb.

Science.gov (United States)

Nikam, Tukaram D; Ghorpade, Ravi P; Nitnaware, Kirti M; Ahire, Mahendra L; Lokhande, Vinayak H; Chopra, Arvind

2013-01-01

Micropropagation through cotyledonary and leaf node and boswellic acid production in stem callus of a woody medicinal endangered tree species Boswellia serrata Roxb. is reported. The response for shoots, roots and callus formation were varied in cotyledonary and leafy nodal explants from in vitro germinated seeds, if inoculated on Murshige and Skoog's (MS) medium fortified with cytokinins and auxins alone or together. A maximum of 8.0 ± 0.1 shoots/cotyledonary node explant and 6.9 ± 0.1 shoots/leafy node explants were produced in 91 and 88 % cultures respectively on medium with 2.5 μM 6-benzyladenine (BA) and 200 mg l(-1) polyvinylpyrrolidone (PVP). Shoots treated with 2.5 μM IBA showed the highest average root number (4.5) and the highest percentage of rooting (89 %). Well rooted plantlets were acclimatized and 76.5 % of the plantlets showed survival upon transfer to field conditions. Randomly amplified polymorphic DNA (RAPD) analysis of the micropropagated plants compared with mother plant revealed true-to-type nature. The four major boswellic acid components in calluses raised from root, stem, cotyledon and leaf explants were analyzed using HPLC. The total content of four boswellic acid components was higher in stem callus obtained on MS with 15.0 μM IAA, 5.0 μM BA and 200 mg l(-1) PVP. The protocol reported can be used for conservation and exploitation of in vitro production of medicinally important non-steroidal anti-inflammatory metabolites of B. serrata.

Micropropagation and validation of genetic and biochemical fidelity among regenerants of Nothapodytes nimmoniana (Graham) Mabb. employing ISSR markers and HPLC.

Science.gov (United States)

Prakash, Lokesh; Middha, Sushil Kumar; Mohanty, Sudipta Kumar; Swamy, Mallappa Kumara

2016-12-01

An in vitro protocol has been established for clonal propagation of Nothapodytes nimmoniana which is an important source of Camptothecin (CPT). Elite source was identified based on the chemical potency to accumulate the optimum level of CPT. Different types and concentrations of plant growth regulators were used to study their effect on inducing multiple shoots from the explants regenerated from embryos of N. nimmoniana. Of these, a combination of N6-benzyladenine (0.2 mg L -1 ) and Indole-3-butyric acid (IBA) (0.1 mg L -1 ) proved optimum for differentiating multiple shoots in 90.6 % of the cultures with an average of 10.24 shoots per explant obtained within 8 weeks of inoculation. Nearly, 92 % of the excised in vitro shoots rooted on half strength Murashige and Skoog (MS) medium containing 0.05 % activated charcoal, supplemented with 1-naphthaleneacetic acid and IBA at 0.1 mg L -1 each. The micropropagated plants were evaluated for their genetic fidelity by employing inter simple sequence repeats (ISSR) markers. Ten individuals, randomly chosen from a population of 145 regenerants, were compared with the donor plant. The regenerated plants were also evaluated for their chemical potency using high-performance liquid chromatography (HPLC) analysis of CPT content. The true-to-type nature of the micropropagated plants was confirmed based on their monomorphic banding profiles with that of the mother plants using ISSR markers. Besides, HPLC evaluation of the CPT content confirmed the existence of chemical uniformity among the regenerated plants and the elite mother plant.

Preculturing effect of thidiazuron on in vitro shoot multiplication and micropropagation round in Capparis decidua (Forsk.) an important multipurpose plant.

Science.gov (United States)

Bukhari, Najat A W; Siddique, Iram; Perveen, Kahkashan

2016-09-01

An efficient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5-100 μM) for different time duration (4, 8, 12 and 16 d), proved a significant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation. The highest regeneration rate (85%), average number of shoots/explant (8.7 ± 0.22) and maximum shoot length (3.9 ± 0.33 cm) were obtained from the nodal explants exposed to 50 μM TDZ for 8 d. The nodal explants excised from the proliferated cultures of TDZ (50 μM) for 8 d were used as explants and showed an enhancement rate after next three round of in vitro propagation. Best results for rooting was obtained by ex vitro treatment of shoots with 200 μM indole-3-butyric acid (IBA) for 20 min. as it produced an average of 5.7 ± 0.41 roots per microshoot with 4.4 ± 0.39 cm root length in 84% shoots. Different planting substrates was tested for maximum survival of hardening off micropropagated plantlets and soilrite proved most effective than others as 97.1 ± 7.21 plantlets survived. All micropropagated plants grew well in natural conditions and showed similar morphology to the mother plant.

[The archeology of slavery on Jesuit fazendas: first research notes].

Science.gov (United States)

Symanski, Luís Cláudio P; Gomes, Flávio

2012-12-01

These preliminary research notes present theoretical and methodological questions regarding a recently inaugurated investigation in historical archeology that intends to analyze daily life under slavery, demographic regimes, cultural practices, and so on. A survey of archeological sites on former 'senzalas' (slave quarters) and slave-owning fazendas in the Paraíba Valley and northern part of the state of Rio de Janeiro is currently in progress. With the cooperation of historians, archeologists, and anthropologists, records of the material culture of slave populations, which originally comprised indigenes and later Africans, are being located at excavations underway on the fazenda that is part of the Jesuit school in Campos dos Goytacazes, Rio de Janeiro, first run by the clergy and later by members of the laity in the seventeenth, eighteenth, and nineteenth centuries.

In the middle of the world, aquendar the methodology: notes for queering curriculum research

Directory of Open Access Journals (Sweden)

Thiago Ranniery Moreira de Oliveira

2016-05-01

Full Text Available This paper investigates how the condition of queer critique not only reflects in the sphere of social exchange, but it can acquire methodological dimensions to the extent that allows reapply to the investigative positions and the paths of curriculum research ironic and jocular style redefinition of the queer insult. The explored argument is that the queer critique allows to question the normative naturalization of research procedures into the curriculum as those contained in the formulas that tend to oppose researcher/researched, realism/fiction, coherence/multiplicity. From the perspective of taking queer as a heterogeneous reading, this text explores how four notes, combinations, wanderings, affectations and fertilizations, translate what happens when queering research methodology. Movements that can be seen as wandering experiments in relation to the paradigm of queer critique in Education and curriculum.

Comparing the Effects of Benzyladenine and meta-Topolin on Sweet Basil (Ocimum basilicum Micropropagation

Directory of Open Access Journals (Sweden)

Szidónia KŐSZEGHI

2014-12-01

Full Text Available Micropropagation of aromatic plants reveals an effective way of obtaining high volume, virus-free plant material of uniform quality. The application of meta-Topolin (mT (N6-(2-hydroxybenzyl adenine-9-riboside and aromatic cytokinin as Benzyladenine (BAP in the micro propagation of sweet basil (Ocimum basilicum L. was tested for the first time and plant growth parameters assessed to determine the optimum level of these cytokinins. Additionally, the rate of root-growth inhibition due to these two cytokinins was also assessed. Our results show that 1 mg/l (4.43 mM BAP and 0.5 mg/l (2.07 mMmT produced the most favourable effects on new shoot developments. Meta-Topolin was shown to increase the quality of the plants and in comparison with BAP fewer distortions were observed. No significant differences in root-growth inhibition between the mT and BAP were detected.  

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Directory of Open Access Journals (Sweden)

Nadjma Souza Leite

2012-10-01

Full Text Available This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7. Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL. Imperial cultivar showed higher bromelain activity than the Pérola's.

Phenotypic aberrations during micropropagation of Soymida febrifuga (Roxb. Adr. Juss

Directory of Open Access Journals (Sweden)

Kishore Kumar CHIRUVELLA

2014-03-01

Full Text Available Like most of the medicinal plants Soymida febrifuga (Meliaceae possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protocols for in vitro mass propagation has been established from field grown and aseptic seedlings explants. Strikingly, we observed aberrant structures such as vitrified shoots, faciated shoots, albino shoots as well shoot necrosis during its micropropagation. These phenotypic maladies were observed during organogenesis and rooting. Compared to other abnormalities, shoot necrosis nonetheless was frequent and pronounced leading to plant death. Shoots when subjected to rooting also displayed necrosis which was controlled by transferring to MS medium containing various concentrations and combinations of calcium levels, activated charcoal, glucose, fructose and auxins. Microshoots initiated roots on half strength MS medium with IBA and IAA individually or in combination within two weeks. MS half strength solid medium supplemented with CAN (556 mg l–1, CAP (1.0 mg l–1, IAA (2.0 mg l–1 and IBA (2.0 mg l–1 in combination was found to be more efficient in showing high frequency (95% of root regeneration. Rooted plantlets were successfully hardened and 70-85% of regenerated plants were successfully acclimatized to natural environment. In vitro derived plantlets were morphologically similar to in vivo plants.

CLONAL MICROPROPAGATION OF POTATO VARIETIES BY WESTERN SIBERIA SELECTION– THE NEW FEATURES

Directory of Open Access Journals (Sweden)

E.P. Miakisheva

2015-04-01

Full Text Available The article is sanctified urgent need to use methods of modern biotechnology in primary seed farming of potatoes in the territory of the Russian Federation. Lack of required amount of good quality planting material is a major factor limiting stable high yield. The crop of potatoes, annually produced in Russia, significantly below global, and does not meet genetic capabilities of the species. Many viral infections tend to accumulate in the tubers, reducing their quality and keeping quality during storage. For the first time peculiarities of clonal micropropagation and in vitro culture of early-maturing potato varieties Lubava Russian breeding (agricultural research Institute of the city of Kemerovo, the Kemerovo region of the Russian Federation. At the stage of introduction to the culture of the parameters, providing pure cultures using lizoformin as sterilizing compounds. At the stage of actually breeding to obtain plants-regenerants of a certain morphological structure was studied the influence of certain components of the nutrient medium. The influence of agar, sucrose and vitamins such morphological indicators of development of regenerated plants as number of internodes and plant height. In the rooting stage the effect of different of growth regulators auxin nature: α-naphthyloxy acid, β-indoleacetic acid and β-indolebutyric acid, in concentrations of 0.1-5 μm. Selected concentrations of these compounds, the introduction of which in the nutrient medium provided the maximum indices of rhisogenesis: the number and length of roots. At the stage of adaptation to non-sterile growing conditions ex vitro the efficiency of the hydroponic plants, filled with a liquid nutrient solution. A successful stage adaptation has characterizability 100% survival rate of plants and increase the morphological characteristics of regenerants.

Review by a local medical research ethics committee of the conduct of approved research projects, by examination of patients' case notes, consent forms, and research records and by interview.

Science.gov (United States)

Smith, T; Moore, E J; Tunstall-Pedoe, H

1997-05-31

To monitor the conduct of medical research projects that have already been approved by the local medical research ethics committee. Follow up study of ethically approved studies (randomly selected from all the studies approved in the previous year) by examination of patients' case notes, consent forms, and research records and by interview of the researchers at their workplace. Tayside, Scotland (mixed rural and urban population). 30 research projects approved by Tayside local medical research ethics committee. Adherence to the agreed protocol, particularly for recruitment (obtaining and recording informed consent) and for specific requirements of the ethics committee, including notification of changes to the protocol and of adverse events. In one project only oral consent had been obtained, and in a quarter of the studies one or more consent forms were incorrectly completed. Inadequate filing of case notes in five studies and of consent forms in six made them unavailable for scrutiny. Adverse events were reported, but there was a general failure to report the abandoning or non-starting of projects in two studies the investigators failed to notify a change in the responsible researcher. Monitoring of medical research by local medical research ethics committees promotes and preserves ethical standards, protects subjects and researchers, discourages fraud, and has the support of investigators. We recommend that 10% of projects should undergo on-site review, with all others monitored by questionnaire. This would require about six person hours of time and a salary bill of 120 pounds per study monitored.

Effect of light in the micropropagation of clones series I.S. - Prunus spp. rootstocks

International Nuclear Information System (INIS)

De Rossi, A.; Rufato, L.; Fiaschi, G.; Morini, S.; Loreti, F.

2004-01-01

The principal problem in the micropropagation of clones from the I.S. Series is the difficulty of internodes elongation. This series of clones was developed in the “Dipartimento de Coltivazione e Difesa delle Specie Legnose G. Scaramuzzi” which belongs to the “Università Degli Studi di Pisa”. The objective of the research was to improve the proliferate and elongation response of the clones explants from the series I.S. 5/18, 5/23 and 5/14 with the application of different light quality, quantity and intensity. Thys, vegetative apices with at least 1 cm of length were used. These explants were submitted to 5 treatments: 1) control (fluorescent light Philips TLD 18W/33), 2) incandescent light, 3) reduction of light intensity (30% less than the control), 4) photoperiod of 16/8 hours and 5) red light. The experimental design used was completely randomized, with 15 replications per treatment. The results were submitted to a comparison of means through the Duncan Test. Sixty days after the on set of the experiment, the average number of developed budding, the length of the internodes, the degree of vitrification and the green color intensity of the explants were evaluated. The treatment using incandescent light has increased the length on the internodes to the clone I.S. 5/18, and also has reduced the vitrification and the intensity of green color on the 3 clones. The red light has reduced the proliferation rate of the clones from the I. S. series. (author) [pt

Efficient micropropagation and assessment of genetic fidelity of Boerhaavia diffusa L- High trade medicinal plant.

Science.gov (United States)

Patil, Kapil S; Bhalsing, Sanjivani R

2015-07-01

Boerhaavia diffusa L is a medicinal herb with immense pharmaceutical significance. The plant is used by many herbalist, Ayurvedic and pharmaceutical industries for production biopharmaceuticals. It is among the 46 medicinal plant species in high trade sourced mainly from wastelands and generally found in temperate regions of the world. However, the commercial bulk of this plant shows genetic variations which are the main constraint to use this plant as medicinal ingredient and to obtain high value products of pharmaceutical interest from this plant. In this study, we have regenerated the plant of Boerhaavia diffusa L through nodal explants and evaluated genetic fidelity of the micropropagated plants of Boerhaavia diffusa L with the help of random amplified polymorphic DNA (RAPD) markers. The results obtained using RAPD showed monomorphic banding pattern revealing genetic stability among the mother plant and in vitro regenerated plants of Boerhaavia diffusa L.

Determine the effective in vitro micro-propagation methods for Tunceli garlic (Allium tuncelianum (Kollman))

International Nuclear Information System (INIS)

2011-01-01

This research was conducted at University of Ankara, Faculty of Agriculture, Department of Horticulture and Turkish Atomic Energy Authority Saraykoey Nuclear Research and Training Center, Plant Breeding Lab's at Agriculture Division within the period of 2004-2006. The scope of this research is to determine the effective in vitro micro-propagation methods as shoot and root culture for Tunceli garlic. In this research to determine the best combinations of growth regulators for shoot and root cultures, 2.4-D and NAA (0, 1.0, 2.0 mgl - 1) were used as auxin and BA (0, 0.1, 1.0 mgl - 1) as cytokinin. No shoots obtained from the leave and root tip culture experiment. Only callus formation was seen in 0.1 mgl - 1 BA+1.0 mgl - 1 NAA combination. Both auxins and cytokinin failed to induce shoot proliferation in Tunceli garlic via shoot culture experiment. Only one shoot was obtained from the 0.05 mg/l BA+0.1 mgl - 1 IAA, 0.1 mgl - 1 BA and 0.1 mgl - 1 BA + 0.1 mgl - 1 NAA, 0.05 mgl - 1 BA+0.5 mgl - 1 IAA and 0.05 mgl - 1 BA combinations. Proliferation frequency increased with lower concentration of IAA with 0.05 mg/l BA. In vitro bulblet formation of garlic shoots started from second subcultures. When the subculture number was increased, also bulbing ratio increased too. Bulb formation ratio was 100% in medium without auxin and 0.1 mgl-NAA+0.1 mgl - 1 BA combination. Shoots do not formed bulblet planted again on medium without auxin or with 0.1 mgl - 1 NAA. Although shoots were started rooting (37%) in without auxin and 40% in 0.1 mgl - 1 NAA from fourth subculture in bulblet formation medium, rooting ratio was found as low level. When unrooted shoots transferred to the medium without auxin and with NAA medium, rooting ratio increased. The highest rooting ratio was 33% in 2 mgl - 1 NAA, and 17% in 0.5 mgl - 1 NAA. When rooted and unrooted garlic bulblets transferred pots, plants died after produced 2-3 leaves but small bulblets were obtained from main bulblets. This is an

Micropropagation and hairy root culture of Ophiorrhiza alata Craib for camptothecin production.

Science.gov (United States)

Ya-ut, Pornwilai; Chareonsap, Piyarat; Sukrong, Suchada

2011-12-01

An efficient system was developed for the in vitro micropropagation and hairy root culture of Ophiorrhiza alata Craib for camptothecin (CPT) production. Shoot multiplication on leaf and node explants from germinated seeds of O. alata was successful on half-strength Murashige and Skoog medium supplemented with varying amounts of kinetin and α-naphthaleneacetic acid. Node explants grown in vitro were successfully infected by Agrobacterium rhizogenes TISTR 1450 for the establishment of hairy root culture. The amount of CPT in various parts of O. alata was analyzed by HPLC. The accumulation of CPT in transformed hairy roots was twice that in soil-grown plants (785 ± 52 and 388 ± 32 μg/g dry wt, respectively). In the presence of a polystyrene resin (Diaion HP-20) that absorbed CPT, the CPT content in the culture media increased sevenfold compared with controls (1,036 and 151 μg per 250 ml medium, respectively). These results enable the feasible production of CPT of O. alata by means of a cell culture strategy. These measures can help safeguard the plant from extinction. © Springer Science+Business Media B.V. 2011

A call for BMC Research Notes contributions promoting best practice in data standardization, sharing and publication.

Science.gov (United States)

Hrynaszkiewicz, Iain

2010-09-02

BMC Research Notes aims to ensure that data files underlying published articles are made available in standard, reusable formats, and the journal is calling for contributions from the scientific community to achieve this goal. Educational Data Notes included in this special series should describe a domain-specific data standard and provide an example data set with the article, or a link to data that are permanently hosted elsewhere. The contributions should also provide some evidence of the data standard's application and preparation guidance that could be used by others wishing to conduct similar experiments. The journal is also keen to receive contributions on broader aspects of scientific data sharing, archiving, and open data.

Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action

International Nuclear Information System (INIS)

Žiauka, Jonas; Kuusienė, Sigutė; Šilininkas, Mindaugas

2013-01-01

Representatives of the genus Populus (poplars), such as Populus tremula L. (European aspen) and its fast-growing hybrids, are recognized as being among the most suitable tree species for short rotation coppicing in Northern Europe. Several technologies have been developed for fast propagation of selected aspen genotypes, including laboratory (in vitro) micropropagation, which is usually based on the action of exogenous plant hormones. Seeking to minimize the use of the latter, the present study was designed to test if the conditions suitable for increased accumulation of plant-produced gas, including the gaseous plant hormone ethylene, inside a culture vessel could contribute to commercially desirable changes in aspen development. Shoot cultures of several European and hybrid (Populus tremuloides Michx. × P. tremula) aspen genotypes were studied using two different types of culture vessels: tightly sealed Petri dishes (15 × 54 mm) designed to provide restricted gas exchange (RGE) conditions, and capped (but not sealed) test tubes (150 × 18 mm) providing control conditions. Under RGE conditions, not only the positive impact of the ethylene precursors 1-aminocyclopropane-1-carboxylic-acid (ACC) and ethephon on shoot proliferation was demonstrated but also a several-fold increase, compared to the control conditions, in the mean shoot number per explant was recorded even on the hormone-free nutrient medium. Moreover, the shoots developed under RGE conditions were distinguished by superior rooting ability in the subsequent culture. These results suggest that a plant micropropagation system based on the action of plant-produced ethylene rather than of exogenous hormones is possible. -- Highlights: ► Aspen in vitro cultures were grown in different vessels. ► Small-volume vessels were used for restriction of gas exchange. ► Aspen explants produced most shoots in small-volume vessels. ► Shoot proliferation was increased due to explant response to ethylene. �

Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micropropagation of Capsicum annuum.

Science.gov (United States)

Asgari-Targhi, Ghasem; Iranbakhsh, Alireza; Ardebili, Zahra Oraghi

2018-06-01

Concerning environmental issues of metal based-nanomaterials and increasing demand for nano-based products; various strategies have been employed to find eco-friendly natural nano-compounds, among which nano-polymer chitosan is mostly considered. Herein, the various aspects of the way in which bulk or nano-chitosan may modify growth, morphogenesis, micropropagation, and physiology of Capsicum annuum L. were considered. Culture medium was manipulated with different concentrations of bulk chitosan or synthesized chitosan/tripolyphosphate (TPP) nano-particle. The supplementations of culture media led to changes in morphology (especially, the root architecture) and differentiation. Toxic doses of bulk (100 mgL -1 ) or nano-chitosan (5, 10, and 20 mgL -1 ) dramatically provoked cessation of plant growth and development. Plant growth and biomass accumulations were increased along with the suitable levels of bulk or nano-chitosan. Peroxidase and catalase activities in a dose and organ-dependent manners were significantly modified by the supplements. Phenylalanine ammonia lyase was induced by the mentioned supplements. Also, the contents of soluble phenols, proline, and alkaloid were found to be significantly increased by the elicitors, over the control. The nano-chitosan of 1 mgL -1 was found to be the most effective elicitor to trigger organogenesis via micropropagation. The huge differences between triggering and toxic concentrations of the supplements would be due to the physicochemical modifications of nano-polymeric. Furthermore, the results highlight the potential benefits (hormone-like activity) and phytotoxic impacts of nano-chitosan/TPP for in vitro manipulations. This is the first report on both the favorable and adverse effects of nano-chitosan/TPP, representing requirements for further investigation on such formulations for future applications. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India.

Science.gov (United States)

Bisht, Shivani; Bisht, N S; Bhandari, Snehlata

2012-01-01

An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25-10.0 mgl(-1)) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5-1.0 mgl(-1)) auxins (NAA/IBA/IAA). 1.0 mgl(-1) BAP with 0.5 mgl(-1) NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl(-1) NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.

Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale.

Science.gov (United States)

Ozel, Cigdem Alev; Khawar, Khalid Mahmood; Unal, Fatma

2015-03-01

Endemic Muscari muscarimi Medikus is the most fragrant plant among Muscari species and has a high ornamental potential. The natural populations of M. muscarimi, are severely affected by increased environmental pollution and urbanization. There is a need to develop a micropropagation method that should serve effectively for commercial propagation and conservation. Therefore, the study targeted to set up a strategy for efficient in vitro bulblet regeneration system of M. muscarimi using twin scale bulb explants on 1.0 × MS medium containing 4.44, 8.88, 17.76 μM BAP (6-Benzylaminopurine) plus 2.685, 5.37, 10.74 μM NAA (α-Naphthalene acetic acid). Maximum number of 19 daughter axillary bulblets and 16 daughter adventitious bulblets per twin bulb scale explant was regenerated on 1.0 × MS medium containing 17.76 μM BAP plus 10.74 μM NAA and 17.76 μM BAP plus 2.685 μM NAA respectively. The daughter bulblets regenerated on twin bulb scales on 8 out of 9 regeneration treatment could be easily rooted on 1.0 × MS medium containing 4.9 μM IBA (Indole-3-butyric acid). The daughter bulblets regenerated on 9th treatment (1.0 × MS medium containing 17.76 μM BAP plus 10.74 μM NAA) were transferred to 1.0 × MS medium containing 30 g/l sucrose to break negative carry over effect of this dose of BAP-NAA, where they grew 2-3 roots of variable length. Daughter bulblet diameter was increased by culturing them on 1.0 × MS medium containing 4.44 μM BAP plus 5.37 μM NAA. The results verified that both age and the source of explants had significant effect on regeneration. In another set of experiments, twin scales were obtained from in vitro regenerated daughter bulblets, although they induced bulblets, yet their bulblet regeneration percentage, mean number of bulblets per explant and their diameter were significantly reduced. In vitro regenerated bulblets were acclimatized in growth chamber under ambient conditions of temperature and humidity on

Mass micropropagation of pineapple tissue culture using bioreactor technology

International Nuclear Information System (INIS)

Irwan Syafri; Amir Hamzah Harun; Rusli Ibrahim

2005-01-01

Pineapple (ananas comosus) is the most important fruit in terms of revenue earner in this country. The export of the canned pineapple is about 2 million standard cases annually valued at RM 60 million, while the export of fresh pineapple is about 40,000 tonnes worth about RM 10 million. The industry for canning is however, an ailing industry with production on the decline since the 70s. Scaling up the pineapple propagation using in vitro methods seems to be possible solutions for the lack of planting material. Temporary immersion system (TIS) has been described by Teisson and Alvard (1995) for plant tissue culture propagation. This system, also known as RITA, has been successfully used with embryogenic tissues of banana (Alvard et al 1993), coffee (Berthouly 1991), rubber (Etienne et al 1993) and sugarcane (Lorenzo et al 1998). In this study, the system has been set up with a potential capacity of 3 manifolds with 10 RITA each, to multiply meristem explants at different immersion periods. The system was compared with the conventional micropropagation system on solid medium. Both systems were treated with MS media containing 2.5 mg/l BAP and 0.1 NAA. In TIS the shoots were able to multiplied faster in comparison with solid media. The multiplication rates were increased up to 1:3 to 1:5 compared to normal propagation on solid media. The results show that TIS not only increase the propagation rates of pineapple but could also be adapted to reduce implementation costs to establish low-cost propagation systems. (Author)

Physicians’ Progress Notes

DEFF Research Database (Denmark)

Bansler, Jørgen; Havn, Erling C.; Mønsted, Troels

2013-01-01

in patient care, they have not dealt specifically with the role, structure, and content of the progress notes. As a consequence, CSCW research has not yet taken fully into account the fact that progress notes are coordinative artifacts of a rather special kind, an open-ended chain of prose texts, written...... sequentially by cooperating physicians for their own use as well as for that of their colleagues. We argue that progress notes are the core of the medical record, in that they marshal and summarize the overwhelming amount of data that is available in the modern hospital environment, and that their narrative...... format is uniquely adequate for the pivotal epistemic aspect of cooperative clinical work: the narrative format enables physicians to not only record ‘facts’ but also—by filtering, interpreting, organizing, and qualifying information—to make sense and act concertedly under conditions of uncertainty...

Effect of explant density and medium culture volumes on cassava micropropagation in Temporal Immersion System

Directory of Open Access Journals (Sweden)

Milagros Basail

2003-04-01

Full Text Available Due to the need of producing high quality planting material available to cassava growers, it has been necessary to look for alternatives in order to increase the efficiancy of in vitro propagation methods and their automation, such as the use of the Temporal Immersion Systems (RITA®. This work was carried out to increase the multiplication coefficient for cassava mass propagation through out Temporal Immersion Systems. The clone ‘CMC-40’ was used. Different medium volumes per explant, and material density per unit at a given Immersion frequency were tested. The highest results were obtained in the 2.8 multiplication coefficient with 20 ml culture medium volume and 3.2 using a density of 40 explants/flask. When the Temporal Immersion System is used with these results, a more efficient method for cassava micropropagation is established and also higher quality vitroplants for the rooting stage and further acclimatization in field conditions are produced. Key Words: Tissue Culture, liquid culture medium, Manihot esculenta Crantz

An Exploration of Note-Taking in an Online Calculus Course

Science.gov (United States)

van de Sande, Carla; Abramson, Jay; Judson-Garcia, Julia

2017-01-01

Taking notes is a popular activity for students attending instructional lectures and has been linked to achievement. Research on note-taking has shown that the contents and usefulness of student notes depends on several factors, including the pace of the lecture and the presence of organizational and selection cues. However, this research has…

Micropropagação, aclimatização, teor e composição química do óleo essencial de genótipos de hortelã japonesa Micropropagation, acclimatization, essential oil content and chemical composition of japanese mint genotypes

Directory of Open Access Journals (Sweden)

Maria de Fátima Arrigoni-Blank

2011-03-01

Full Text Available A hortelã japonesa (Mentha arvensis é uma espécie aromática, originária do sul da China, o seu óleo essencial é rico em mentol, o qual é empregado nas indústrias farmacêuticas, de alimentos e de cosméticos. Este trabalho teve como objetivo desenvolver um protocolo para a micropropagação e aclimatização de genótipos de hortelã japonesa, além de analisar a composição química dos óleos essenciais de plantas micropropagadas e não micropropagadas. Segmentos nodais são os mais indicados para a micropropagação dos genótipos de M. arvensis. A concentração de 4,4 µM de AIA promoveu um maior número de brotos e folhas por explante do genótipo MA701-02, em relação aos demais genótipos. A utilização de 4,4 µM AIA e 9,3 µM CIN + 8,9 µM BAP + 2,2 µM AIA proporcionou maior acúmulo de massa seca no genótipo MA701-02, e no MA701-04 foi a concentração de 8,9 µM BAP + 5,4 µM ANA. O substrato pó de coco + 1 g L-1 de calcário + 12 g L-1 de Biosafra® (3-12-6 pode ser indicado para a aclimatização de mudas micropropagadas dos genótipos de hortelã japonesa avaliados. A micropropagação não alterou o teor de óleo essencial e foram identificados 17 constituintes químicos, perfazendo um total de 92 a 99%.Japanese mint (Mentha arvensis L. is an aromatic species originated from South China and its essential oil is rich in menthol, which is used in the pharmaceutical, food and cosmetic industries. The aim of this work was to develop a protocol for micropropagation and acclimatization of japanese mint genotypes and analyze the chemical composition of the essential oils from micropropagated and not micropropagated plants. Nodal segments are the most indicated for micropropagation of the M. arvensis genotypes. The concentration of 4.4 µM of IAA promoved higher number of shoots and leaves per explant of the MA701-02 genotype when compared with the others. The use of 4,4 µM of IAA and 9,3 µM of KIN + 8,9 µM of BAP + 2

Lagos Notes and Records

African Journals Online (AJOL)

PROMOTING ACCESS TO AFRICAN RESEARCH ... Lagos Notes and Records is an annual, interdisciplinary journal of the humanities. ... Insuring the Nation: Europeans and the Emergence of Modern Insurance Business in Colonial Nigeria ...

Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants.

Science.gov (United States)

Caro, L A; Polci, P A; Lindström, L I; Echenique, C V; Hernández, L F

2002-04-01

Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P. chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1-2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successful with cuttings taken from young or adult plants, but explants from young plants showed a better response. Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.

Micropropagation of ginger (zingiber officinale var. rubrum) using buds from microshoots

International Nuclear Information System (INIS)

Zuraida, A.R.

2016-01-01

Zingiber officinale var. Rubrum (ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2). (author)

Plant germination and production of callus from the yellow hornpoppy (Glaucium flavum): the first stage of micropropagation.

Science.gov (United States)

Mohamed, M E; Arafa, A M; Soliman, S S; Eldahmy, S I

2014-09-01

The yellow hornpoppy, Glaucium flavum Cr. (Fam. Papaveraceae) is a perennial herb, distributed in the Mediterranean region, including Egypt. The plant contains many benzyl isoquinoline alkaloids from the aporphine type such as glaucine, isoboldine, 1-chelidonine, 1-norchelidonine and 3-O-methylarterenol, making it to display various medicinal activities including antitussive, anticancer, antioxidant, antimicrobial, antiviral, hypoglycemic, analgesic, antipyretic, bronchodilator and anti-inflammatory effects. The plant is now rare and endangered in the Egyptian flora due to urban sprawl. The present study looks into Glaucium flavum seeds' in vitro germination as well as the ability of the explants taken from the growing seedlings to form stable callus lines in order to enable micropropagation as a way to save the rare plant. The study also scans the production of different medicinally valuable alkaloids, particularly glaucine, in produced callus.

A simple and cost effective liquid culture system for the micropropagation of two commercially important apple rootstocks.

Science.gov (United States)

Mehta, Mohina; Ram, Raja; Bhattacharya, Amita

2014-07-01

The two commercially important apple rootstocks i.e., MM106 and B9 were micropropagated using a liquid culture system. Three different strengths of 0.8% agar solidified PGR free basal MS medium were first tested to optimize the culture media for both the rootstocks. Full strength medium (MS0) supported maximum in vitro growth, multiplication, rooting and survival under field conditions as opposed to quarter and half strength media. When three different volumes of liquid MS0 were tested, highest in vitro growth, multiplication, rooting and also survival under field conditions were achieved in 20 mL liquid MS0. The cost of one litre of liquid medium was also reduced by 8 times to Rs. 6.29 as compared to solid medium. The cost of 20 mL medium was further reduced to Rs. 0.125.

OpenLabNotes

DEFF Research Database (Denmark)

List, Markus; Franz, Michael; Tan, Qihua

2015-01-01

be advantageous if an ELN was Integrated with a laboratory information management system to allow for a comprehensive documentation of experimental work including the location of samples that were used in a particular experiment. Here, we present OpenLabNotes, which adds state-of-the-art ELN capabilities to Open......LabFramework, a powerful and flexible laboratory information management system. In contrast to comparable solutions, it allows to protect the intellectual property of its users by offering data protection with digital signatures. OpenLabNotes effectively Closes the gap between research documentation and sample management......, thus making Open-Lab Framework more attractive for laboratories that seek to increase productivity through electronic data management....

Micropropagation effect on the anti-carcinogenic activitiy of polyphenolics from Mexican oregano (Poliomintha glabrescens Gray) in human colon cancer cells HT-29.

Science.gov (United States)

García-Pérez, Enrique; Noratto, Giuliana D; García-Lara, Silverio; Gutiérrez-Uribe, Janet A; Mertens-Talcott, Susanne U

2013-06-01

Phenolic extracts obtained from spices are known to have anti-carcinogenic activities but little is known about the effect of micropropagation on these beneficial effects. The main objective of this study was to evaluate the cytotoxic activity of flavonoid-enriched extracts (FEE) from the leaves of wild (WT), in vitro (IN), and ex vitro (EX) grown oregano plants in colon cancer cells HT-29 and the non-cancer cells CCD-18Co. Cell proliferation of HT-29 cells was reduced to 50 % by WT, IN, and EX at concentrations of 4.01, 1.32, and 4.84 mg of gallic acid equivalents (GAE)/L, respectively. In contrast, in CCD-18Co cells, higher concentrations were required for the same cytotoxic effect. At 6 mg GAE/L, WT and IN reduced the production of reactive oxygen species (ROS) of lipopolysaccharides (LPS)-stimulated control cells to 59.89 and 59.43 %, respectively, and EX to 73.89 %. The mRNA of Caspase-3 was increased 1.53-fold when cells were treated with 4 mg GAE/L of IN extract, and tumor necrosis factor receptor superfamily, member 6 (FAS), and BCL2-associated X protein (BAX) mRNA increased 2.55 and 1.53 fold, respectively. Results on protein expression corroborated the apoptotic effects with a significant decrease of B-cell lymphoma 2 (BCL2) expression for all treatments but more remarkable for EX that also showed the most intense signal of BAX. Overall, FEE extracts derived from micropropagation had increased pro-apoptotic effects, however extracts from the in vitro plants produced more efficacy at the transcriptional level while extracts from the ex vitro plant were superior at the traductional level.

Note Taking and Note Sharing While Browsing Campaign Information

DEFF Research Database (Denmark)

Robertson, Scott P.; Vatrapu, Ravi; Abraham, George

2009-01-01

Participants were observed while searching and browsing the internet for campaign information in a mock-voting situation in three online note-taking conditions: No Notes, Private Notes, and Shared Notes. Note taking significantly influenced the manner in which participants browsed for information...

Effect of Different Concentrations of Growth Regulators on Gardenia jasminoides cv. Veitchii Micropropagation by Tissue Culture Technique

Directory of Open Access Journals (Sweden)

G. R. Abdullah

2003-01-01

Full Text Available Micropropagation techniques were set up for Gardenia jasminoides c.v. veitchi. Many plantlets were obtained by culturing shoot cuttings in MS nutrient media, 30 g/L Sucrose, 7 g/L Agar Agar, and different concentrations of BAP and IAA. The best concentration was 1mg /L BAP with 0.5 mg/L IAA. This concentration gave the best sprout growth suitable for rooting in primary and secondary culture by reculturing the stuck cutting every 6 weeks and for many times. We also obtained a high rooting percentage up to 98 % of natural rooting in rooting media different from propagation media by reducing mineral salt concentration to half, Sucrose to 20gm/L, and 2gm/L active charcoal, and 1mg/L IAA. Plantlets were transferred to greenhous and subjected for hardening. This technique gave 22 plantlets from one cutting in one year.

Parallel Note-Taking: A Strategy for Effective Use of Webnotes

Science.gov (United States)

Pardini, Eleanor A.; Domizi, Denise P.; Forbes, Daniel A.; Pettis, Gretchen V.

2005-01-01

Many instructors supply online lecture notes but little attention has been given to how students can make the best use of this resource. Based on observations of student difficulties with these notes, a strategy called parallel note-taking was developed for using online notes. The strategy is a hybrid of research-proven strategies for effective…

Tissue culture as a plant production technique for horticultural crops

African Journals Online (AJOL)

STORAGESEVER

2009-08-18

Aug 18, 2009 ... Recovery of regenerants from transformed cells. - Cell culture .... methods. Micropropagation techniques. Micropropagation is a simple concept. The basic pro- tocols were well established by the 1960s and a whole research field and ... the environment are naturally contaminated on their sur- faces (and ...

Eliciting Student Thinking: Definition, Research Support, and Measurement of the "ETS"® National Observational Teaching Examination (NOTE) Assessment Series. Research Memorandum No. RM-16-06

Science.gov (United States)

Qi, Yi; Sykes, Gary

2016-01-01

This report describes and provides research and scholarly support for a core practice of teaching--eliciting student thinking (EST)--that is the target for a performance assessment contributing one component of the "ETS"® National Observational Teaching Examination (NOTE) assessment series. The purpose of this report is to review the…

Suicide Notes in Hong Kong in 2000

Science.gov (United States)

Wong, Paul W. C.; Yeung, April W. M.; Chan, Wincy S. C.; Yip, Paul S. F.; Tang, Arthur K. H.

2009-01-01

Suicide notes have been regarded as one of the most informative data sources to understand the reasons why people commit suicide. However, there is a paucity of suicide note studies, leaving researchers with an assumption that this phenomenon remains static over time. This study examines this assumption by comparing the characteristics of…

Effect of Sucrose and Growth Regulator’s Level on Ginger Micropropagation

Directory of Open Access Journals (Sweden)

Resham Babu Amgai

2017-05-01

Full Text Available Ginger is most important cash crop of the hilly region of Nepal. However, availability of disease free planting material (rhizome is the major problem faced by Nepalese farmers. Tissue culture is the only option to produce disease free rhizome of ginger. Suitable culture media combination is most important for the production of planting material in ginger through tissue culture. Therefore, effect of different level of sucrose and growth regulators on micro-propagation of ginger was studied using local collection ‘Kaski Local’. Early stage bud was used as explant. MS basal media with different level of sucrose and growth regulators was used as tissue culture media. 30 g/L sucrose, 30 g/L sucrose+5mg/L BA, 30 g/L sucrose+5 mg/L BA+0.5 mg/L NAA, 60 g/L sucrose+5mg/L BA, 60 g/L sucrose+5 mg/L BA+0.5mg/L NAA, 90 g/L sucrose+5 mg/L BA was used in this study. The explants were surface sterilized, cultured and incubated at 25±2°C, 90-95% relative humidity and 14:10 hours light:dark photoperiod for 8 weeks. Increased level of the sucrose increased the rhizome weight, however, addition of NAA produced more positive effect for this. MS basal media with 60 g/L sucrose+5 mg/L BA+0.5 mg/L NAA produced higher rhizome weight.

Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse.

Science.gov (United States)

Zakavi, Maryam; Askari, Hossein; Irvani, Neda

2016-10-01

The present study concentrated on introducing a micropropagation protocol for a drought resistant genotype from Pyrus boissieriana , which is the second most naturally widespread pear species in Iran with proper physiological and medicinal properties. Proliferating microshoot cultures were obtained by placing nodal segments on MS medium supplemented with BAP and IBA or NAA. The highest number of shoots (27 shoots per explant) were obtained with 1.5 mg l -1 BAP and 0.05 mg l -1 IBA, but this combination did not produce shoots of desirable length (>1.7 cm). Combination of 1.75 mg l -1 BAP and 0.07 mg l -1 IBA was the best for the shoot multiplication in P. boissieriana with a sufficient number of shoot production (22.33 shoots per explant) and relatively more appropriate shoot length. The larger and greenish leaves were obtained when PG was added to the best multiplication treatment. Microshoot elongation was carried out in 1/2 and 1/4 MS medium containing 50-100 mg l -1 PG with different concentrations of IBA or NAA at intervals of 30-60 days. Significant increase in shoot length was detected after 45-60 days of culture in the presence of PG. The highest shoot length (8 cm) was recorded on 1/2 MS medium supplemented with 0.5 mg l -1 IBA and 100 mg l -1 PG. GA 3 negatively affected number and length of shoots and generally caused generation of red leaves. The highest percentage of root induction (100%) and root length (9 cm) were obtained on 1/6 strength MS medium supplemented with 0.005 mg l -1 IBA. All plantlets were hardened when transferred to ex vitro conditions through a period of 25-30 days. The results suggest axillary shoot proliferation of P. boissieriana could successfully be employed for propagation of candidate drought resistant seedling.

Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir.

Science.gov (United States)

Khalafalla, Mutasim M; Daffalla, Hussien M; Abdellatef, Eltayb; Agabna, Elsadig; El-Shemy, Hany A

2011-04-01

This report describes in vitro micropropagation of Boscia senegalensis, so-called famine foods, that helped the people in Darfur and Kordofan, Sudan survive during the 1984-1985 famine. Four types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1-5 mg/L 6-benzyladenine (BA). The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA, while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L BA. The shoot cluster, when subcultured to its same medium, significantly increased the rate of shoot multiplication by the end of the third subculture. The maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium. In vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete plantlets. B. senegalensis-regenerated plantlets obtained in vitro for the first time, were hardened and 95% survived under greenhouse conditions.

Meristem micropropagation of cassava (Manihot esculenta) evokes genome-wide changes in DNA methylation.

Science.gov (United States)

Kitimu, Shedrack R; Taylor, Julian; March, Timothy J; Tairo, Fred; Wilkinson, Mike J; Rodríguez López, Carlos M

2015-01-01

There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic changes during propagation by meristem culture and by field cuttings in five cassava (Manihot esculenta) cultivars. Principal Co-ordinate Analysis of profiles generated by methylation-sensitive amplified polymorphism revealed clear divergence between samples taken from field-grown cuttings and those recovered from meristem culture. There was also good separation between the tissues of field samples but this effect was less distinct among the meristem culture materials. Application of methylation-sensitive Genotype by sequencing identified 105 candidate epimarks that distinguish between field cutting and meristem culture samples. Cross referencing the sequences of these epimarks to the draft cassava genome revealed 102 sites associated with genes whose homologs have been implicated in a range of fundamental biological processes including cell differentiation, development, sugar metabolism, DNA methylation, stress response, photosynthesis, and transposon activation. We explore the relevance of these findings for the selection of micropropagation systems for use on this and other crops.

Micropropagation and protein profile analysis by SDS-PAGE of Gracilaria changii (Rhodophyta, Solieriaceae

Directory of Open Access Journals (Sweden)

Lin Wei Jong

2015-05-01

Full Text Available Gracilaria changii seaweed is primarily important as a source of agar with wide applications in food industries. The high demand of agar led to gradual depletion of G. changii in natural resources. Establishment of in vitro culture of G. changii has an important role and allowing G. changii explants to grow optimally under controlled conditions to provide constant, continuous and sufficient seedlings supply for Gracilaria farming. This study focused on micropropagation culture of G. changii in which different exogenous factors influencing seaweed growth were investigated: strength of chosen medium Provasoli’s enriched seawater (PES, types and concentration of fertilizers/biostimulant, supplementation of plant growth regulators and seawater salinity. The results were presented in daily growth rate of explants and data analysis was carried out using one-way ANOVA. The results demonstrated high growth rate of G. changii in 25% of PES supplemented with 5 mg L−1 AMPEP, and seawater salinity range between 30 and 40 ppt, respectively. Protein profiles of tissue-cultured and farm cultivated G. changii were produced by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE. The results demonstrated no remarkable difference in the protein profiles and indicated the suitability of the culture condition for the growth of G. changii.

Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers.

Science.gov (United States)

Goswami, Komal; Sharma, R; Singh, P K; Singh, Govind

2013-01-01

A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l(-1)) or kinetin (0.5 mg l(-1)). BA was recorded to be better than kinetin in terms of number of days taken to bud break. The maximum number of shoots per explant was observed on 0.1 mg l(-1) BA and 0.5 mg l(-1) kinetin. Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (0.1 mg l(-1) each), it increased with increasing concentration of BA up to 10.0 mg l(-1). None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation. In the case of a combination of BA + kinetin + IBA, the maximum (5.5 shoots per explants) proliferation was observed on MS medium containing 1.0 mg l(-1) BA + 0.5 mg l(-1) kinetin + 0.5 mg l(-1) IBA or 0.25 mg l(-1) BA + 1.0 mg l(-1) kinetin + 1.0 mg l(-1) IBA. Regenerated shoots showed root induction on MS basal medium or on MS medium containing 1.0 mg l(-1) IBA. It is concluded that a five-fold increase (1.0 mg l(-1) BA + 0.5 mg l(-1) kinetin + 0.5 mg l(-1) IBA) in axillary shoot proliferation, while seven-fold increase (0.25 mg/l mg l(-1) BA + 1.0 mg l(-1) kinetin + 1.0 mg l(-1) IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations. PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication.

Micropropagação de Cabralea canjerana Micropropagation of Cabralea canjerana

Directory of Open Access Journals (Sweden)

Silvana Cruz da Rocha

2007-02-01

Full Text Available A Cabralea canjerana (Vell. Mart. (Meliaceae (canjarana é uma espécie arbórea nativa brasileira importante para fornecimento de madeira de boa qualidade. As sementes desta espécie não podem ser armazenadas por muito tempo e, por tanto, existe a necessidade do desenvolvimento de técnicas alternativas de propagação como a micropropagação. Neste trabalho, foram realizados experimentos de multiplicação utilizando segmentos nodais, retirados de plantas germinadas in vitro. Os segmentos foram inoculados em meio de cultura MS ou WPM, adicionado de 6-benzilaminopurina (BAP e, ou, 2-isopenteniladenina (2-iP nas concentrações de 2,5 ou 5 µM. Microestacas de rebrotas foram colocadas em meio de cultura MS/2, com a metade da concentração dos sais do meio MS, adicionado de ácido indol 3-butírico (AIB (0, 2,5 e 5 µM. Após sete dias, foram transferidas para meio MS/2 sem auxina e na luz. Na fase de multiplicação, o meio de cultura MS foi mais adequado que o meio WPM. O segmento nodal, em presença de 2,5 µM de BAP, propiciou um dos melhores resultados, com uma taxa de multiplicação de 1,77 por mês, em meio de cultura MS. O enraizamento das microestacas oriundas de rebrotas foi de 87,5% em presença de 5 µM de AIB durante sete dias. A aclimatização foi realizada em casa de vegetação e proporcionou 90% de sobrevivência das mudas após 30 dias. A micropropagação da canjarana a partir de segmentos nodais de mudas cultivadas in vitro é viável para a multiplicação dessa espécie.Cabralea canjerana (Vell. Mart. (Meliaceae ("canjarana" is a native tree of economic importance in Brazil. The storage of seeds is of short duration and it is therefore necessary to establish a protocol for micropropagation of this species. In this work, multiplication experiments were carried out using nodal segments, excised from in vitro germinated plants. The segments were inoculated in MS or WPM culture medium, supplemented with 6-benzylaminopurine

Modeling and Explaining Content: Definition, Research Support, and Measurement of the "ETS"® National Observational Teaching Examination (NOTE) Assessment Series. Research Memorandum No. RM-16-07

Science.gov (United States)

Stickler, Leslie; Sykes, Gary

2016-01-01

This report reviews the scholarly and research evidence supporting the construct labeled modeling and explaining content (MEC), which is measured via a performance assessment in the "ETS"® National Observational Teaching Examination (NOTE) assessment series. This construct involves practices at the heart of teaching that deal with how…

Factors Influencing the Effectiveness of Note Taking on Computer-Based Graphic Organizers

Science.gov (United States)

Crooks, Steven M.; White, David R.; Barnard, Lucy

2007-01-01

Previous research on graphic organizer (GO) note taking has shown that this method is most effective when the GO is presented to the student partially complete with provided notes. This study extended prior research by investigating the effects of provided note type (summary vs. verbatim) and GO bite size (large vs. small) on the transfer…

Sealing and explant types on the mangaba micropropagation Tipo de vedação e explantes na micropropagação de mangabeira

Directory of Open Access Journals (Sweden)

Aline de Jesus Sá

2012-08-01

Full Text Available In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L-1 IAA and 1 mg L-1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro cultivation. The best types of flask sealing for the establishment phase were the PVC film and Para-film® and for the first subculture the Para-film® seal. In the second subculture the PVC film and Para-film® seals promoted the best growth. The median and basal nodal segments presented the best performance in the first subculture. No significant effect of explant type was observed in the second subculture. The ideal subculture interval in the establishment phase and the first and second subcultures is 50 days.Na micropropagação, especialmente para mangaba, variedade botânica da árvore do Nordeste do Brasil, aspectos limitantes, como acúmulo de etileno no recipiente de cultivo e perda de vigor em subculturas têm sido observados. Neste estudo, objetivou-se avaliar o conhecimento técnico e científico da propagação in vitro de mangabeira, variedade botânica do Nordeste do Brasil, e melhorar o protocolo de micropropagação, o melhor tipo de vedação frasco e explante em diferentes etapas. Para a fase de estabelecimento e para as subculturas primeiro e segundo, foi utlizado o meio MS com 3% de sacarose e agar 0,6%, suplementado com 1 mg L-1 de AIA e 1 mg L-1 de BAP. As avaliações foram

Insights regarding the Usefulness of Partial Notes in Mathematics Courses

Science.gov (United States)

Cardetti, Fabiana; Khamsemanan, Nirattaya; Orgnero, M. Carolina

2010-01-01

Note-taking is a widespread practice used by college students to record information from lectures. Unfortunately, even successful students' notes are incomplete and, therefore, may lack the potential to positively impact their academic performance. Research suggests that instructors can help students improve their note-taking skills by using…

Suicide note themes and suicide prevention.

Science.gov (United States)

Foster, Tom

2003-01-01

The aim was to determine if suicide note themes might inform suicide prevention strategies. The themes of 42 suicide notes from the Northern Ireland Suicide Study (major psychological autopsy study) were examined. The commonest themes were "apology/shame" (74%), "love for those left behind" (60%), "life too much to bear" (48%), "instructions regarding practical affairs post-mortem" (36%), "hopelessness/nothing to live for" (21%) and "advice for those left behind" (21%). Notes of suicides with major unipolar depression were more likely than notes of suicides without major unipolar depression to contain the themes "instructions regarding practical affairs post-mortem" (67% versus 19%, p = 0.005) and "hopelessness/nothing to live for" (40% versus 11%, p = 0.049). Notes of suicides with a previous history of deliberate self-harm were less likely than notes of suicides without a history of deliberate self-harm to contain the theme "apology/shame" (58% versus 87%, p = 0.04). Notes of elderly suicides were more likely than non-elderly notes to contain the theme "burden to others" (40% versus 3%, p = 0.03). The fact that three quarters of suicide notes contained the theme "apology/shame" suggests that the deceased may have welcomed alternative solutions for their predicaments. Scrutiny of suicide note themes in the light of previous research findings suggests that cognitive therapy techniques, especially problem solving, may have an important role to play in suicide prevention and that potential major unipolar depressive (possibly less impulsive) suicides, in particular, may provide fertile ground for therapeutic intervention (physical and psychological). Ideally all primary care doctors and mental health professionals working with (potentially) suicidal people should be familiar with basic cognitive therapy techniques, especially problem solving skills training.

Maintaining knowledge, training and infrastructure for research and development in nuclear safety. A note by the International Nuclear Safety Advisory Group

International Nuclear Information System (INIS)

International Nuclear Safety Advisory Group

2001-01-01

The purpose of this INSAG Note is to emphasize the importance of maintaining capabilities for nuclear research and education, especially with regard to safety aspects, so that nuclear safety may be maintained in IAEA Member States, and to alert Member States to the potential for significant harm if the infrastructure for research, development and education is not maintained

Note-taking in the employment interview: effects on recall and judgments.

Science.gov (United States)

Middendorf, Catherine Houdek; Macan, Therese Hoff

2002-04-01

Although note-taking in the employment interview is highly recommended, little research has examined its effects. This study investigated the effects of note-taking styles, review of the notes, and content of the notes on participants' cued recall of information and decisions made from videotaped employment interviews. Note-taking increased recall accuracy but not judgment accuracy. Being able to review notes resulted in increased judgment accuracy for those taking conventional-style notes. The content of the notes also had important implications for conventional note-takers, suggesting some benefits of recording notes using the key-points style. The findings suggest that the act of note-taking may be more important for memory and legal reasons than for improving the decisions made by interviewers.

GeneNotes – A novel information management software for biologists

Directory of Open Access Journals (Sweden)

Wong Wing H

2005-02-01

Full Text Available Abstract Background Collecting and managing information is a challenging task in a genome-wide profiling research project. Most databases and online computational tools require a direct human involvement. Information and computational results are presented in various multimedia formats (e.g., text, image, PDF, word files, etc., many of which cannot be automatically processed by computers in biologically meaningful ways. In addition, the quality of computational results is far from perfect and requires nontrivial manual examination. The timely selection, integration and interpretation of heterogeneous biological information still heavily rely on the sensibility of biologists. Biologists often feel overwhelmed by the huge amount of and the great diversity of distributed heterogeneous biological information. Description We developed an information management application called GeneNotes. GeneNotes is the first application that allows users to collect and manage multimedia biological information about genes/ESTs. GeneNotes provides an integrated environment for users to surf the Internet, collect notes for genes/ESTs, and retrieve notes. GeneNotes is supported by a server that integrates gene annotations from many major databases (e.g., HGNC, MGI, etc.. GeneNotes uses the integrated gene annotations to (a identify genes given various types of gene IDs (e.g., RefSeq ID, GenBank ID, etc., and (b provide quick views of genes. GeneNotes is free for academic usage. The program and the tutorials are available at: http://bayes.fas.harvard.edu/genenotes/. Conclusions GeneNotes provides a novel human-computer interface to assist researchers to collect and manage biological information. It also provides a platform for studying how users behave when they manipulate biological information. The results of such study can lead to innovation of more intelligent human-computer interfaces that greatly shorten the cycle of biology research.

Natural-orifice transluminal endoscopic surgery (NOTES) in Europe: summary of the working group reports of the Euro-NOTES meeting 2010

DEFF Research Database (Denmark)

Meining, A; Feussner, H; Swain, P

2011-01-01

The fourth Euro-NOTES workshop took place in September 2010 and focused on enabling intensive scientific dialogue and interaction between participants to discuss the state of the practice and development of natural-orifice transluminal endoscopic surgery (NOTES) in Europe. Five working groups were...... collaboration and indications, robotics and platforms, and matters related to training and education. This review summarizes consensus statements of the working groups to give an overview of what has been achieved so far and what might be relevant for research related to NOTES in the near future....

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

Science.gov (United States)

Reis, Ricardo S.; Heringer, Angelo S.; Rangel, Patricia L.; Santa-Catarina, Claudete; Grativol, Clícia; Veiga, Carlos F. M.; Souza-Filho, Gonçalo A.

2017-01-01

Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress. PMID:28419154

A xyloglucan from seeds of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples.

Science.gov (United States)

Lima-Nishimura, N; Quoirin, M; Naddaf, Y G; Wilhelm, H M; Ribas, L L F; Sierakowski, M-R

2003-01-01

Xyloglucan was extracted from seeds of Hymenaea courbaril and mixed with agar to prepare a solid culture medium used for micropropagation of the Marubakaido apple rootstock (Malus prunifolia Borkh) and cv. Jonagored (Malus domestica). The performance on gels created from a blend of 0.4%agar and 0.2% xyloglucan (w/v) was compared with that on media gelled with a standard concentration 0.6% (w/v) of agar. The growth of shoots and the multiplication rate were higher on the modified culture medium than on the agar-gelled medium. The occurrence of hyperhydric shoots was lower on the modified medium. In the absence of auxin, shoot rooting reached 70% (Marubakaido) and 66% (Jonagored) on the agar-xyloglucan medium and 6.7% and 10.4%, respectively, on the agar medium. When 0.25 microM indole-3-butyric acid (IBA) was added to both media, the modified medium gave better results in terms of rooting percentage and quality of roots than the agar-gelled medium.

Comparing the Effects of Benzyladenine and meta-Topolin on Sweet Basil (Ocimum basilicum Micropropagation

Directory of Open Access Journals (Sweden)

Szidónia KŐSZEGHI

2014-12-01

Full Text Available Micropropagation of aromatic plants reveals an effective way of obtaining high volume, virus-free plant material of uniform quality. The application of meta-Topolin (mT (N6-(2-hydroxybenzyl adenine-9-riboside and aromatic cytokinin as Benzyladenine (BAP in the micro propagation of sweet basil (Ocimum basilicum L. was tested for the first time and plant growth parameters assessed to determine the optimum level of these cytokinins. Additionally, the rate of root-growth inhibition due to these two cytokinins was also assessed. Our results show that 1 mg/l (4.43 mM BAP and 0.5 mg/l (2.07 mMmT produced the most favourable effects on new shoot developments. Meta-Topolin was shown to increase the quality of the plants and in comparison with BAP fewer distortions were observed. No significant differences in root-growth inhibition between the mT and BAP were detected.

Monitoring the infective process of the downy mildew causal agent within micropropagated rose plants

Directory of Open Access Journals (Sweden)

Sonia Yamile Gómez

2012-08-01

Full Text Available Downy mildew in the rose caused by a species of the Peronospora genus is a very restrictive disease for the Colombian greenhouse rose production. The damage observed in the susceptible varieties of commercial rose include symptoms affect young steams and tiny leaves causing reddish and brown spots and defoliation; leading to 10% production losses. The infective behavior of this pathogen was studied with the aim of increasing the knowledge about the biology of the rose downy mildew. The study of the infective process was performed on the Charlotte variety using micropropagated roses inoculated with suspensions of sporangia. A germinal tube was observed during the germination process, it came from a lateral papilla and reached up to 300 microns in length. During this study, the ability of the pathogen to use vascular sieves as communication systems within the plant was determined. Oogonia and antheridia were also observed inside the epidermal cells, and oospores inside the parenchymal tissue close to xylem vessels. To the best of our knowledge, these sexual structures have not been reported on in Colombia before. This study verifies the ability of the downy mildew causal agent to move through the xylem vessels and produce sexual structures, such as oogonia, antheridia and oospores within those tissues.

Meristem micropropagation of cassava (Manihot esculenta evokes genome-wide changes in DNA methylation

Directory of Open Access Journals (Sweden)

Shedrack Reuben Kitimu

2015-08-01

Full Text Available There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic changes during propagation by meristem culture and by field cuttings in five cassava (Manihot esculenta cultivars. Principal Co-ordinate Analysis of profiles generated by Methylation Sensitive Amplified Polymorphism (MSAP revealed clear divergence between samples taken from field-grown cuttings and those recovered from meristem culture. There was also good separation between the tissues of field samples but this effect was less distinct among the meristem culture materials. Application of methylation-sensitive Genotype By Sequencing (msGBS identified 105 candidate epimarks that distinguish between field cutting and meristem culture samples. Cross referencing the sequences of these epimarks to the draft cassava genome revealed 102 sites associated with genes whose homologues have been implicated in a range of fundamental biological processes including cell differentiation, development, sugar metabolism, DNA methylation, stress response, photosynthesis, and transposon activation. We explore the relevance of these findings for the selection of micropropagation systems for use on this and other crops.

Digital Note-Taking: Discussion of Evidence and Best Practices.

Science.gov (United States)

Grahame, Jason A

2016-03-01

Balancing active course engagement and comprehension with producing quality lecture notes is challenging. Although evidence suggests that handwritten note-taking may improve comprehension and learning outcomes, many students still self-report a preference for digital note-taking and a belief that it is beneficial. Future research is warranted to determine the effects on performance of digitally writing notes. Independent of the methods or software chosen, best practices should be provided to students with information to help them consciously make an educated decision based on the evidence and their personal preference. Optimal note-taking requires self-discipline, focused attention, sufficient working memory, thoughtful rewording, and decreased distractions. Familiarity with the tools and mediums they choose will help students maximize working memory, produce better notes, and aid in their retention of material presented.

36 CFR 1254.12 - Will NARA log or inspect my computer, other equipment, and notes?

Science.gov (United States)

2010-07-01

... personal notes into the research room. In research rooms that permit taking in your notes, a NARA or... computer, other equipment, and notes? 1254.12 Section 1254.12 Parks, Forests, and Public Property NATIONAL... MATERIALS General Information § 1254.12 Will NARA log or inspect my computer, other equipment, and notes? (a...

NOTES: issues and technical details with introduction of NOTES into a small general surgery residency program.

Science.gov (United States)

Kavic, Michael S; Mirza, Brian; Horne, Walter; Moskowitz, Jesse B

2008-01-01

laparoscopic observation of NOTES maneuvers. Necropsy was performed to determine specific details of surgical intervention. NOTES intervention is feasible in an animal model. Insight into the potential of NOTES was obtained in this investigation. NOTES investigation in a controlled, laboratory setting using an animal model proved to have value for our program. A steep learning curve was encountered despite the availability of an investigator familiar with elementary NOTES procedures. The authors strongly suggest investigators adopt the ASGE/SAGES working group recommendations for a multidisciplinary team possessing advanced therapeutic endoscopic and advanced laparoscopic skills to study NOTES before human investigation. Animal laboratory facilities to perform research and training should be available to the multidisciplinary team for exploration of NOTES techniques and procedures. Institutional Review Board (IRB) approval must be obtained before introduction of NOTES procedures in human patients.

Implications of observing and writing field notes through different lenses

Directory of Open Access Journals (Sweden)

Hellesø R

2015-04-01

Full Text Available Ragnhild Hellesø,1 Line Melby,1 Solveig Hauge21Department of Nursing Science, Institute of Health and Society, Faculty of Medicine, University of Oslo, Oslo, Norway; 2Faculty of Health and Social Studies, Telemark University College, Porsgrunn, NorwayBackground: From a philosophy of science perspective, the literature has posited that different research approaches influence field studies. Studies addressing interdisciplinary research have focused on the challenges of organizing and running interdisciplinary teams, cultural differences between and within disciplines, and constraints in conducting interdisciplinary research. Studies exploring and discussing the process and outcome of transferring observations to notes from an interdisciplinary point of view are not identified. The aim of this paper is to explore the characteristics of field notes created by researchers representing different disciplines and experiences.Methods: A case study using a modified dynamic observation method was employed. The analyses were initiated by a researcher who had not been involved in the data collection. The field notes were analyzed using three main steps.Results: The structures of both researchers' field notes were characterized by similarities in their descriptions, but the notes' foci and analytical levels differed.Conclusion: The findings contribute new insights concerning the execution of interdisciplinary observational studies. Our findings demonstrate that entering the field with different lenses produced richer and more varied data, providing a broader platform from which to discuss and interpret a study's findings. From a theoretical point of view, the findings enable a more nuanced discussion and a conceptual elaboration regarding how observational approaches should be pursued in future studies. On a practical level, the findings show that even if the researchers agree on what the overall focus in the observations should be, differences can occur in

The pen is mightier than the keyboard: advantages of longhand over laptop note taking.

Science.gov (United States)

Mueller, Pam A; Oppenheimer, Daniel M

2014-06-01

Taking notes on laptops rather than in longhand is increasingly common. Many researchers have suggested that laptop note taking is less effective than longhand note taking for learning. Prior studies have primarily focused on students' capacity for multitasking and distraction when using laptops. The present research suggests that even when laptops are used solely to take notes, they may still be impairing learning because their use results in shallower processing. In three studies, we found that students who took notes on laptops performed worse on conceptual questions than students who took notes longhand. We show that whereas taking more notes can be beneficial, laptop note takers' tendency to transcribe lectures verbatim rather than processing information and reframing it in their own words is detrimental to learning. © The Author(s) 2014.

Medical students' note-taking in a medical biochemistry course: an initial exploration.

Science.gov (United States)

Morrison, Elizabeth H; McLaughlin, Calvin; Rucker, Lloyd

2002-04-01

Beginning medical students spend numerous hours every week attending basic science lectures and taking notes. Medical faculty often wonder whether they should give students pre-printed instructors' notes before lectures. Proponents of this strategy argue that provided notes enhance learning by facilitating the accurate transmission of information, while opponents counter that provided notes inhibit students' cognitive processing or even discourage students from attending lectures. Little if any research has directly addressed medical students' note-taking or the value of providing instructors' notes. The educational literature does suggest that taking lecture notes enhances university students' learning. University students perform best on post-lecture testing if they review a combination of provided notes and their own personal notes, particularly if the provided notes follow a 'skeletal' format that encourages active note-taking.

Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

Directory of Open Access Journals (Sweden)

Shahril Efzueni Rozali

2014-01-01

Full Text Available A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v NaOCl, 70% (v/v ethanol, and 0.3% (w/v HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP, 1.0 mg/L 1-Naphthaleneacetic acid (NAA, 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA compared to indolebutyric acid (IBA. The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Science.gov (United States)

Rozali, Shahril Efzueni; Rashid, Kamaludin A; Taha, Rosna Mat

2014-01-01

A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Suicide Notes in Mexico: What Do They Tell Us?

Science.gov (United States)

Chavez-Hernandez, Ana-Maria; Paramo, Daniel; Leenaars, Antoon A.; Leenaars, Lindsey

2006-01-01

According to international and Mexican official statistics, there is a dramatic rise in suicide in Mexico; however, research in this area is severely limited. This is the first study of suicide notes from Mexico in the international literature. From a population of 747 registered suicides, a sample of 106 note-writers and 106 nonnote writers was…

Research Note: The consequences of different methods for handling missing network data in Stochastic Actor Based Models.

Science.gov (United States)

Hipp, John R; Wang, Cheng; Butts, Carter T; Jose, Rupa; Lakon, Cynthia M

2015-05-01

Although stochastic actor based models (e.g., as implemented in the SIENA software program) are growing in popularity as a technique for estimating longitudinal network data, a relatively understudied issue is the consequence of missing network data for longitudinal analysis. We explore this issue in our research note by utilizing data from four schools in an existing dataset (the AddHealth dataset) over three time points, assessing the substantive consequences of using four different strategies for addressing missing network data. The results indicate that whereas some measures in such models are estimated relatively robustly regardless of the strategy chosen for addressing missing network data, some of the substantive conclusions will differ based on the missing data strategy chosen. These results have important implications for this burgeoning applied research area, implying that researchers should more carefully consider how they address missing data when estimating such models.

Clinical Psychology and Research: epistemological notes

Directory of Open Access Journals (Sweden)

Emanuela Coppola

2013-05-01

Full Text Available The paper proposes a reflection on the relationship between clinical psychology and research, highlighting the constant epistemological crossing the two practices, empirical and professional. The paper warns against the pitfalls of reductionism that, in both cases, may impact the effectiveness of therapeutic results. In fact, both in clinical practice and is in psychological research, the mere application of techniques contradicts the specificity of the object of study (the mind which, rather, requires the constant attention to a complexity of variables and contextual elements essential for the understanding the psychic. Qualitative research has been a prolific space for dialogue and joint trials between research and clinical practice that has rehabilitated scientific dignity of affective and subjective for a long time confined to the ephemeral world of poetry and literature. It must therefore be a further extension of the convergence not only of qualitative and quantitative methods but also of training modules for researchers and practitioners are able to stimulate, in daily practice, confidence in the utility of scientific monitoring and detection of inter-subjective variables in research devices.

A Micropropagation Protocol for a Critically Endangered Mangrove Excoecaria Agallocha L

Directory of Open Access Journals (Sweden)

Panneerselvam RAJARAM

2012-06-01

Full Text Available Excoecaria agallocha L. is a critically endangered mangrove tree from the Pichavaram mangrove reserve forest, the Tamil Nadu Coastal area. It is distributed on the seashore and the edge-mangrove. In order to reduce the decrease in number of these Mediterranean mixed stand, unsupervised forest management practices have drastically been reduced. In addition, the deforestation of the mangrove area, along with a low seed germination rate further endanger this species. In this study we developed a protocol for the micropropagation of adult Excoecaria agallocha. Microcuttings were obtained from lateral and apical twigs of mature plants and used as explants. Microcuttings with axillary buds were grown on different media, plant growth regulators and phenolic exudation substances. The axillary shoots produced on uncontaminated explants were excised, segmented and recultured in the same medium, to increase the stock of shoot cultures. The Modified Murashige and Skoog (MMS medium, augmented with different concentrations of N6 – benzyl adenine (BAP and Naphthalene acetic acid (NAA, either alone, or in combinations, as a potential medium for shoot multiplication by nodal segments, was tested. In the following experiment, equal molar concentrations of four cytokonins [BAP, Kinetin and 2- isopenthenyladenine (2iP] in combination with equal molar concentrations of three auxins [ NAA, Indole acetic acid (IAA and indole-3- butyric ]were used to test the rate of axillary shoot proliferation, induced on MMS agar medium supplemented with 3.9 µM BAP and 1.34 µM NAA after 6 weeks in culture. Different auxins (NAA, IBA and IAA were to determine the optimum conditions for in vitro rooting of microshoots. The best results were accomplished with NAA 5.41 µM (89% rooting and with IBA at 2.85 or 5.71µM (86% and 86.5% rooting, respectively.

Note-Taking Skills of Middle School Students with and without Learning Disabilities

Science.gov (United States)

Boyle, Joseph R.

2010-01-01

For middle school students with learning disabilities (LD), one major component of learning in content area classes, such as science, involves listening to lectures and recording notes. Lecture learning and note-taking are critical skills for students to succeed in these classes. Despite the importance of note-taking skills, no research has been…

Research Note: Yavirau: A traditional Fijian fish drive as an example of culturally embedded community development

Directory of Open Access Journals (Sweden)

Fink, Michael

2012-09-01

Full Text Available A yavirau (traditional Fijian fish drive is an ancient Fijian custom which has been adapted to today’s needs. Implemented and organised by a village community without external assistance, this highly this culturally specific custom is an example of development on a local level. According to theorists and practitioners working on development issues, such a strategy for Community Development (CD is promising because it seizes current approaches as it fosters local, decentralised, cultural specific development and aims at a high level of local participation. This research note analyses a yavirau as an example of CD, showing its advantages as well as its limitations.

Revising Lecture Notes: How Revision, Pauses, and Partners Affect Note Taking and Achievement

Science.gov (United States)

Luo, Linlin; Kiewra, Kenneth A.; Samuelson, Lydia

2016-01-01

Note taking has been categorized as a two-stage process: the recording of notes and the review of notes. We contend that note taking might best involve a three-stage process where the missing stage is revision. This study investigated the benefits of revising lecture notes and addressed two questions: First, is revision more effective than…

Who Leaves Suicide Notes? An Exploration of Victim Characteristics and Suicide Method of Completed Suicides in Queensland.

Science.gov (United States)

Carpenter, Belinda; Bond, Christine; Tait, Gordon; Wilson, Moira; White, Kris

2016-01-01

The objective of this study is to address the question: are those who leave suicide notes representative of the larger population of those who commit suicide? The method involves an analysis of a full population of suicides by residents of Queensland, Australia for the full year of 2004, with the information drawn from Coronial files. Our overall results suggest that, and in support of previous research, the population who leaves suicide notes are remarkably similar to those who do not. Differences are identified in four areas: first, and in contrast to prior research, females are less likely to leave a suicide note; second, and in support of previous research, Aboriginal Australians are less likely to leave suicide notes; third, and in support of some previous research, those who use gas as a method of suicide are more likely to leave notes, while those who use a vehicle or a train are less likely to leave notes; finally, our findings lend support to research which finds that those with a diagnosed mental illness are less likely to leave notes. The discussion addresses some of the reasons these disparities may have occurred, and continues the debate over the degree to which suicide notes give insight into the larger suicide population.

Editorial note

DEFF Research Database (Denmark)

Gani, Rafiqul; Hrymak, A.; Lee, J.

2009-01-01

industrial and educational applications. These articles will highlight theory, models, algorithms and applications with respect to value preservation and/or value creation or growth within the chemical product supply chain. To highlight and motivate research in the emerging challenges in PSE, we plan...... components of PSE—modeling, numerical analysis, optimization, systems and control theory, computer science, and, management science will be highlighted through the published articles (full-length papers, perspective papers, review papers, short notes and letters to the editor). They will cover...... and intelligent systems, integrated approaches to design, control and data analysis, systematic techniques for managing complexity, etc.), PSE emerging domains (product-process design, enterprise-wide optimization, energy and sustainability, biological engineering, pharmaceutical engineering, etc.) and novel...

Notes on Commercialization of Biotechnology Research

DEFF Research Database (Denmark)

Høy Jakobsen, Palle

This book introduces aspects of commercialization of biomedical & biotech research including pharmaceutical drug and medical device development, innovation concepts, patent protection & commercialisation of research by securing investments in new companies and by licensing of the commersialisatio...

Detecting clinically relevant new information in clinical notes across specialties and settings.

Science.gov (United States)

Zhang, Rui; Pakhomov, Serguei V S; Arsoniadis, Elliot G; Lee, Janet T; Wang, Yan; Melton, Genevieve B

2017-07-05

Automated methods for identifying clinically relevant new versus redundant information in electronic health record (EHR) clinical notes is useful for clinicians and researchers involved in patient care and clinical research, respectively. We evaluated methods to automatically identify clinically relevant new information in clinical notes, and compared the quantity of redundant information across specialties and clinical settings. Statistical language models augmented with semantic similarity measures were evaluated as a means to detect and quantify clinically relevant new and redundant information over longitudinal clinical notes for a given patient. A corpus of 591 progress notes over 40 inpatient admissions was annotated for new information longitudinally by physicians to generate a reference standard. Note redundancy between various specialties was evaluated on 71,021 outpatient notes and 64,695 inpatient notes from 500 solid organ transplant patients (April 2015 through August 2015). Our best method achieved at best performance of 0.87 recall, 0.62 precision, and 0.72 F-measure. Addition of semantic similarity metrics compared to baseline improved recall but otherwise resulted in similar performance. While outpatient and inpatient notes had relatively similar levels of high redundancy (61% and 68%, respectively), redundancy differed by author specialty with mean redundancy of 75%, 66%, 57%, and 55% observed in pediatric, internal medicine, psychiatry and surgical notes, respectively. Automated techniques with statistical language models for detecting redundant versus clinically relevant new information in clinical notes do not improve with the addition of semantic similarity measures. While levels of redundancy seem relatively similar in the inpatient and ambulatory settings in the Fairview Health Services, clinical note redundancy appears to vary significantly with different medical specialties.

The Impact of Guided Notes on Post-Secondary Student Achievement: A Meta-Analysis

Science.gov (United States)

Larwin, Karen H.; Larwin, David A.

2013-01-01

The common practice of using of guided notes in the post-secondary classroom is not fully appreciated or understood. In an effort to add to the existing research about this phenomenon, the current investigation expands on previously published research and one previously published meta-analysis that examined the impact of guided notes on…

Enhanced micropropagation and tiller formation in sugarcane through pretreatment of explants with thidiazuron (TDZ).

Science.gov (United States)

Kumari, Kavita; Lal, Madan; Saxena, Sangeeta

2017-10-01

An efficient, simple and commercially applicable protocol for rapid micropropagation of sugarcane has been designed using variety Co 05011. Pretreatment of shoot tip explants with thidiazuron (TDZ) induced high frequency regeneration of shoot cultures with improved multiplication ratio. The highest frequency (80%) of shoot initiation in explants pretreated with 10 mg/l of TDZ was obtained during the study. Maximum 65% shoot cultures could be established from the explants pretreated with TDZ as compared to minimum 40% establishment in explants without pretreatment. The explants pretreated with 10 mg/l of TDZ required minimum 40 days for the establishment of shoot cultures as compared to untreated explants which required 60 days. The highest average number of shoots per culture (19.1) could be obtained from the explants pretreated with 10 mg/l of TDZ, indicating the highest multiplication ratio (1:6). Highest rooting (over 94%) was obtained in shoots regenerated from pretreated explants on ½ strength MS medium containing 5.0 mg/l of NAA and 50 g/l of sucrose within 15 days. Higher number of tillers/clump (15.3) could be counted in plants regenerated from pretreated explants than untreated ones (10.9 tillers/clump) in field condition, three months after transplantation. Molecular analysis using RAPD and DAMD markers suggested that the pretreatment of explants with TDZ did not adversely affect the genetic stability of regenerated plants and maintained high clonal purity.

A regenerative route for Eugenia uniflora L. (Myrtaceae through in vitro germination and micropropagation

Directory of Open Access Journals (Sweden)

Paulo Roberto Diniz da Silva

2014-07-01

Full Text Available Eugenia uniflora is a tree species native from Central and South America, largely employed in the popular medicine, in the cosmetics and pharmaceutical industries and also consumed in natura. Aiming to provide plant material with high sanity and genetic uniformity for the establishment of commercial plantations, we developed a protocol for seeds disinfestation, in vitro germination and in vitro propagation of this species through organogenesis. Fruits of E. uniflora were obtained from wild trees growing in the Pampa biome, Southern Brazil. Seeds were disinfested using ethanol 70% (10 min and NaOCl 1.25% (10 or 25 min. Shoot apexes and nodal segments of non-contaminated plantlets were cultivated in verification medium AS30 during 20 days, posteriorly in ½MS medium supplemented with sucrose, IBA and BAP during 45 days and acclimatized in greenhouse. Disinfesting seeds with ethanol 70% (10 min and NaOCl 1.25% (25 min allowed germination with significantly lower contamination (2.0% and production of healthy explants for the micropropagation. No difference concerning size and contamination was observed for the propagation using shoot apexes or nodal segments as explant. Acclimatized plants revealed normal phenotype and healthy appearance. This regenerative route can be applied for mass clonal propagation from seeds of cross-pollinated or self-pollinated selected trees aiming the establishment of commercial plantations of E. uniflora and other Myrtaceae species.

Micropropagation of Codiaeum variegatum (L.) Blume and regeneration induction via adventitious buds and somatic embryogenesis.

Science.gov (United States)

Radice, Silvia

2010-01-01

Codiaeum variegatum (L) Blume cv. "Corazon de oro" and cv. "Norma" are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots. The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected.Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton. On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too. Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants. On the other hand, leaf-sectioned of C. variegatum cv. Norma does not start somatic embryo differentiation if 1 mg/L TDZ is not added to the MS basal medium. Incipient callus is observed after 30 days of culture, and then, subculture to MS with 1 mg/L BA allows the same process to show on the "Corazon de oro" cultivar. Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free basal medium with activated charcoal. Root induction is possible on basal medium plus 1 mg/L IBA. Plantlets in the greenhouse have variegated leaves true-to-type.

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants.

Science.gov (United States)

Shinde, Smita; Sebastian, Joseph Kadanthottu; Jain, Jyothi Ramesh; Hanamanthagouda, Manohar Shirugumbi; Murthy, Hosakatte Niranjana

2016-10-01

A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 µM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was sub-cultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 µM) along with 7.5 µM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 µM BAP + 7.5 µM 2-iP. Quarter strength MS medium supplemented with 10 µM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.

A note on notes: note taking and containment.

Science.gov (United States)

Levine, Howard B

2007-07-01

In extreme situations of massive projective identification, both the analyst and the patient may come to share a fantasy or belief that his or her own psychic reality will be annihilated if the psychic reality of the other is accepted or adopted (Britton 1998). In the example of' Dr. M and his patient, the paradoxical dilemma around note taking had highly specific transference meanings; it was not simply an instance of the generalized human response of distracted attention that Freud (1912) had spoken of, nor was it the destabilization of analytic functioning that I tried to describe in my work with Mr. L. Whether such meanings will always exist in these situations remains a matter to be determined by further clinical experience. In reopening a dialogue about note taking during sessions, I have attempted to move the discussion away from categorical injunctions about what analysis should or should not do, and instead to foster a more nuanced, dynamic, and pair-specific consideration of the analyst's functioning in the immediate context of the analytic relationship. There is, of course, a wide variety of listening styles among analysts, and each analyst's mental functioning may be affected differently by each patient whom the analyst sees. I have raised many questions in the hopes of stimulating an expanded discussion that will allow us to share our experiences and perhaps reach additional conclusions. Further consideration may lead us to decide whether note taking may have very different meanings for other analysts and analyst-patient pairs, and whether it may serve useful functions in addition to the one that I have described.

The Circular Economy and the Leading European Retailers: A Research Note

Directory of Open Access Journals (Sweden)

Peter Jones

2018-03-01

Full Text Available The concept of the circular economy is gaining momentum in political and business thinking about the transition to a more sustainable future. EuroCommerce and the European Retail Round Table, for example, have argued that leading retailers are keen to play a leading role in shaping the circular economy within Europe. This exploratory research note outlines the characteristic features of the concept of the circular economy, provides some illustrations of how Europe’s leading retailers are publicly addressing circular economy approaches and offers some general reflections on the application of the concept within the retail sector of the economy. The findings reveal that almost 50% of the leading European retailers signalled a commitment to the circular economy and to the principles underpinning it and a number of them looked to evidence their commitment within their retail operations. That said the authors suggest that If Europe’s leading retailers’ public commitments to a more circular economy are to become a reality then they will not only need to effect a radical change in their current business models and that this will need to be accompanied by radical changes in consumers consumption behaviour. More contentiously, there must be concerns that the leading European retailers might effectively capture the concept of the circular economy to justify continuing economic growth.

NOTE TAKING PAIRS TO IMPROVE STUDENTS‟ SENTENCE BASED WRITING ACHIEVEMENT

Directory of Open Access Journals (Sweden)

Testiana Deni Wijayatiningsih

2017-04-01

Full Text Available Students had skill to actualize their imagination and interpret their knowledge through writing which could be combined with good writing structure. Moreover, their writing skill still had low motivation and had not reached the standard writing structure. Based on the background above, this research has purpose to know the influence Note Taking Pairs in improving students‘sentence based writing achievement. The subject of this research was the second semester of English Department in Muhammadiyah University of Semarang. It also used statistic non parametric method to analyze the students‘ writing achievement. The result of this research showed that Note Taking Pairs strategy could improve students‘sentence based writing achievement. Hopefully this research is recommended into learning process to improve students‘writing skill especially in sentence-based writing subject.

Producción de caña semilla en Semilleros Registrados provenientes de micropropagación y de hidrotermoterapia de tres cultivares de caña de azúcar Seedcane production in Registered Nurseries obtained through micropropagation and hot water treatment of three sugarcane cultivars

Directory of Open Access Journals (Sweden)

Patricia A. Digonzelli

2006-12-01

Full Text Available Se evaluaron los componentes de la producción de caña semilla en Semilleros Registrados de caña de azúcar provenientes de micropropagación e hidrotermoterapia (50ºC, 2 h, en tres variedades (LCP 85-384, CP 65-357 y CP 48-103 y tres épocas de plantación (mayo, agosto y octubre. La semilla micropropagada y la hidrotermotratada estaban libres de achaparramiento de la caña soca (Leifsonia xyli subsp. xyli o RSD, por sus siglas en inglés, y escaldadura de la hoja (Xanthomonas albilineans. El diseño experimental fue un factorial completamente aleatorizado 3x3x2, con tres repeticiones. Los factores fueron: época de plantación (tres niveles, variedad (tres niveles y origen de la semilla (dos niveles. Cada parcela constaba de cinco surcos de 3 metros, evaluando los tres surcos centrales. En la cosecha del semillero se contaron todos los tallos por parcela y se midió su altura hasta hoja TVD. En una muestra de 45 tallos por parcela se determinaron el peso y número de yemas por tallo. A partir de los datos obtenidos se calculó el número de yemas por surco de 100 metros de longitud y por hectárea. El origen de la caña semilla (micropropagada o hidrotermotratada afectó el número de tallos y la altura y número de yemas por tallo, por surco y por hectárea. La época de plantación y la variedad afectaron los componentes de la producción de caña semilla. La micropropagada presentó una mayor población, altura y número de yemas por tallo, permitiendo aumentar entre el 8% y 23% el área que es posible plantar a partir de una hectárea de Semillero Registrado. La magnitud de las diferencias entre la caña semilla micropropagada y termotratada varió según cultivares y época de plantación.Seedcane yield components in sugarcane Registered Nurseries obtained through micropropagation and hot water treatment (50ºC, 2 h were evaluated, considering three varieties (LCP 85-384, CP 65-357 and CP 48-103 and three plantation dates (May, August

Coping with Stress. Research Notes.

Science.gov (United States)

Jordan, Debra J.

1995-01-01

Research related to the impact of exercise on stress indicates that a regular aerobic exercise program is important to control the negative effects of stress. It was also reported that those who are physically fit have higher levels of self-esteem. Implications for camp staff involve starting a regular exercise program to offset job-related…

The Impact of Structured Note Taking Strategies on Math Achievement of Middle School Students

Science.gov (United States)

Wilkinson, Gregory Ashley

2012-01-01

Student math achievement continues to be a national, state, and local concern. Research suggests that note taking can improve academic achievement, but current research has failed to report how low achievers might benefit from using note taking during math classes. The purpose of this study was to determine if teaching students structured note…

Open Oncology Notes: A Qualitative Study of Oncology Patients' Experiences Reading Their Cancer Care Notes.

Science.gov (United States)

Kayastha, Neha; Pollak, Kathryn I; LeBlanc, Thomas W

2018-04-01

Electronic medical records increasingly allow patients access to clinician notes. Although most believe that open notes benefits patients, some suggest negative consequences. Little is known about the experiences of patients with cancer reading their medical notes; thus we aimed to describe this qualitatively. We interviewed 20 adults with metastatic or incurable cancer receiving cancer treatment. The semistructured qualitative interviews included four segments: assessing their overall experience reading notes, discussing how notes affected their cancer care experiences, reading a real note with the interviewer, and making suggestions for improvement. We used a constant comparison approach to analyze these qualitative data. We found four themes. Patients reported that notes resulted in the following: (1) increased comprehension; (2) ameliorated uncertainty, relieved anxiety, and facilitated control; (3) increased trust; and (4) for a subset of patients, increased anxiety. Patients described increased comprehension because notes refreshed their memory and clarified their understanding of visits. This helped mitigate the unfamiliarity of cancer, addressing uncertainty and relieving anxiety. Notes facilitated control, empowering patients to ask clinicians more questions. The transparency of notes also increased trust in clinicians. For a subset of patients, however, notes were emotionally difficult to read and raised concerns. Patients identified medical jargon and repetition in notes as areas for improvement. Most patients thought that reading notes improved their care experiences. A small subset of patients experienced increased distress. As reading notes becomes a routine part of the patient experience, physicians might want to elicit and address concerns that arise from notes, thereby further engaging patients in their care.

Adaptive changes in photosynthetic performance and secondary metabolites during white dead nettle micropropagation.

Science.gov (United States)

Kapchina-Toteva, V; Dimitrova, M A; Stefanova, M; Koleva, D; Kostov, K; Yordanova, Zh P; Stefanov, D; Zhiponova, M K

2014-09-15

The white dead nettle, Lamium album L., is an herb that has been successfully cultivated under in vitro conditions. The L. album micropropagation system offers a combination of factors (light intensity, temperature, carbon dioxide (CO2) level, humidity) that are limiting for plant growth and bioactive capacity. To get a better understanding of the mechanism of plant acclimation towards environmental changes, we performed a comparative investigation on primary and secondary metabolism in fully expanded L. album leaves during the consecutive growth in in situ, in vitro, and ex vitro conditions. Although the genetic identity was not affected, structural and physiological deviations were observed, and the level of bioactive compounds was modified. During in vitro cultivation, the L. album leaves became thinner with unaffected overall leaf organization, but with a reduced number of palisade mesophyll layers. Structural deviation of the thylakoid membrane system was detected. In addition, the photosystem 2 (PS2) electron transport was retarded, and the plants were more vulnerable to light damage as indicated by the decreased photoprotection ability estimated by fluorescence parameters. The related CO2 assimilation and transpiration rates were subsequently reduced, as were the content of essential oils and phenolics. Transfer of the plants ex vitro did not increase the number of palisade numbers, but the chloroplast structure and PS2 functionality were recovered. Strikingly, the rates of CO2 assimilation and transpiration were increased compared to in situ control plants. While the phenolics content reached normal levels during ex vitro growth, the essential oils remained low. Overall, our study broadens the understanding about the nature of plant responses towards environmental conditions. Copyright © 2014 Elsevier GmbH. All rights reserved.

Front-Row Seat at the IPY: The Field Notes Electronic Newsletter

Science.gov (United States)

Rithner, P. K.; Zager, S. D.; Garcia-Lavigne, D. N.

2007-12-01

As employees of Polar Field Services/VPR, the arctic logistics provider to the US National Science Foundation, we bear witness to the exploration, documentation, and celebration of the International Polar Year (IPY). Our front- row vantage point (logisticians working with field scientists) offers us a rare opportunity to report on developments at the frontiers of polar research and to describe how scientists work in the Arctic. Our reporting mechanism is field notes, a weekly (summer) to monthly (winter) electronic digest of information about the IPY research we support. Each issue showcases a short "cover" piece highlighting science projects or profiling arctic program participants. In addition, field notes offers news updates, short interviews, and blog-style dispatches contributed by researchers and support personnel. Wherever possible, we include URLs so readers may find more information via the Web: we link to an online database of projects we maintain for the NSF, to university Web sites, project blogs, and so on. We aim to inform the interested layperson about the myriad of activity in the IPY. We like to show that arctic science is interesting, relevant--and a great adventure. We've found field notes to be an excellent outreach venue. By no means a slick media outlet, field notes is published "on the side" by a small but dedicated group of employees who are endlessly fascinated by, and who enjoy an engaging perspective on, contemporary arctic research. Newsletter

The future of NOTES from the conservative point of view.

Science.gov (United States)

Kano, Nobuyasu

2009-01-01

Recently, the unfamiliar term "natural orifice transluminal endoscopic surgery (NOTES)" appeared in my field. Actually, I am hesitant to accept this technique in my surgical practice. In this paper, I will review some references and try to establish my position toward NOTES. The author has been skeptical and ironical about the clinical potentiality of NOTES since hearing the presentation about NOTES for the first time. I have been concerned about making a puncture in the gastrointestinal tract as an old surgeon who believes that intestinal injury must not occur during surgery. However, recent advances in the research of NOTES are changing my stubborn belief. What I have to do is to avoid interrupting or disturbing young surgeons' challenges to develop NOTES. I remember that some senior surgeons were against us when we started laparoscopic surgery around 1990. Senior surgeons and physicians must be generous, considerate, helpful and supportive to our followers. I have been enthusiastic about the development and spread of laparoscopic surgery since 1987 and have been doing various surgical procedures myself, including those involving the biliary tract, pancreas, spleen, upper and lower gastrointestinal tract, adrenal, kidney and gynecologic organs. Recently, the unfamiliar term "NOTES" appeared in my field. Actually, I am hesitant to accept this technique in my surgical practice. In this paper I will review some references and try to establish my position toward NOTES.

Improvement noted after a multifaceted approach to diabetes ...

African Journals Online (AJOL)

guidelines by clinicians was also noted in other studies carried out in the United States of ... Health Ethics Review (UHERB) and the Biomedical Research. Ethics Committee ..... results of feasibility study. The DCCT ... there a gap? The South ...

Performance of the diffraction grating on a bank note: the experience with the Australian Commemorative note

Science.gov (United States)

Hardwick, Bruce A.

1990-04-01

In January 1988, we at the Note Printing Branch first released to the Reserve Bank a polymer based banknote containing an Optically Variable Device (OVD), a diffraction grating. This banknote was the culmination of many years of Research and Development effort, and represented a very significant departure from conventional banknote technology in many ways. It was a test bed. We are very pleased with its performance.

Identification of documented medication non-adherence in physician notes.

Science.gov (United States)

Turchin, Alexander; Wheeler, Holly I; Labreche, Matthew; Chu, Julia T; Pendergrass, Merri L; Einbinder, Jonathan S; Einbinder, Jonathan Seth

2008-11-06

Medication non-adherence is common and the physicians awareness of it may be an important factor in clinical decision making. Few sources of data on physician awareness of medication non-adherence are available. We have designed an algorithm to identify documentation of medication non-adherence in the text of physician notes. The algorithm recognizes eight semantic classes of documentation of medication non-adherence. We evaluated the algorithm against manual ratings of 200 randomly selected notes of hypertensive patients. The algorithm detected 89% of the notes with documented medication non-adherence with specificity of 84.7% and positive predictive value of 80.2%. In a larger dataset of 1,000 documents, notes that documented medication non-adherence were more likely to report significantly elevated systolic (15.3% vs. 9.0%; p = 0.002) and diastolic (4.1% vs. 1.9%; p = 0.03) blood pressure. This novel clinically validated tool expands the range of information on medication non-adherence available to researchers.

1. On note taking.

Science.gov (United States)

Plaut, Alfred B J

2005-02-01

In this paper the author explores the theoretical and technical issues relating to taking notes of analytic sessions, using an introspective approach. The paper discusses the lack of a consistent approach to note taking amongst analysts and sets out to demonstrate that systematic note taking can be helpful to the analyst. The author describes his discovery that an initial phase where as much data was recorded as possible did not prove to be reliably helpful in clinical work and initially actively interfered with recall in subsequent sessions. The impact of the nature of the analytic session itself and the focus of the analyst's interest on recall is discussed. The author then describes how he modified his note taking technique to classify information from sessions into four categories which enabled the analyst to select which information to record in notes. The characteristics of memory and its constructive nature are discussed in relation to the problems that arise in making accurate notes of analytic sessions.

What happens when patients can see their doctors' note? - the Open Notes movement

OpenAIRE

Mende, Susan

2017-01-01

Introduction:  The Open Notes movement represents a culture change, enabling patients’ access to their providers’ notes, thereby increasing transparency and patient engagement.Policy context, objective and highlights:  OpenNotes involves allowing patients on-line or hard copy access to their providers’ notes. The one-year initial pilot began in 2010 with twenty thousand patients and one hundred primary care physicians at three medical centers in the United States.  The pilot’s evaluation foun...

Note Taking and Recall

Science.gov (United States)

Fisher, Judith L.; Harris, Mary B.

1974-01-01

To study the effect of note taking and opportunity for review on subsequent recall, 88 college students were randomly assigned to five treatment groups utilizing different note taking and review combinations. No treatment effects were found, although quality of notes was positively correlated with free recall an multiple-choice measures.…

The Composer's Program Note for Newly Written Classical Music: Content and Intentions.

Science.gov (United States)

Blom, Diana M; Bennett, Dawn; Stevenson, Ian

2016-01-01

In concerts of western classical music the provision of a program note is a widespread practice dating back to the 18th century and still commonly in use. Program notes tend to inform listeners and performers about historical context, composer biographical details, and compositional thinking. However, the scant program note research conducted to date reveals that program notes may not foster understanding or enhance listener enjoyment as previously assumed. In the case of canonic works, performers and listeners may already be familiar with much of the program note information. This is not so in the case of newly composed works, which formed the basis of the exploratory study reported here. This article reports the views of 17 living contemporary composers on their writing of program notes for their own works. In particular, the study sought to understand the intended recipient, role and the content of composer-written program notes. Participating composers identified three main roles for their program notes: to shape a performer's interpretation of the work; to guide, engage or direct the listener and/or performer; and as collaborative mode of communication between the composer, performer, and listener. For some composers, this collaboration was intended to result in "performative listening" in which listeners were actively engaged in bringing each composition to life. This was also described as a form of empathy that results in the co-construction of the musical experience. Overall, composers avoided giving too much personal information and they provided performers with more structural information. However, composers did not agree on whether the same information should be provided to both performers and listeners. Composers' responses problematize the view of a program note as a simple statement from writer to recipient, indicating instead a more complex set of relations at play between composer, performer, listener, and the work itself. These relations are

Sphagnum restoration on degraded blanket and raised bogs in the UK using micropropagated source material: a review of progress

Directory of Open Access Journals (Sweden)

S.J.M Caporn

2018-05-01

Full Text Available There is a growing demand for a supply of Sphagnum to re-introduce to degraded peatlands. However, available supplies of Sphagnum of the desired species are often limited. We describe the propagation of Sphagnum from vegetative material in sterile tissue culture and the introduction of juvenile mosses into the field. Sphagnum produced in the laboratory in three different forms (beads, gel and plugs was introduced to different peatland surfaces on upland degraded blanket bog and lowland cut-over peatland in northern England. On degraded blanket bog, the establishment of mixed-species Sphagnum plugs was typically 99 % while the survival of beads was much lower, ranging from little above zero on bare eroding peat to a maximum of 12 % on stabilised peat surfaces. On lowland cut-over peatland, all trials took place on peat with an expanding cover of Eriophorum angustifolium and tested Sphagnum gel as well as beads and plugs. This work showed that survival and establishment of plugs was high (99 % and greater than for beads. Sphagnum gel reached a cover of 95 % in two years. The vegetative micropropagation of Sphagnum offers an effective source of Sphagnum for re-introduction to degraded peatlands.

Euro-NOTES Status Paper: from the concept to clinical practice.

Science.gov (United States)

Fuchs, K H; Meining, A; von Renteln, D; Fernandez-Esparrach, G; Breithaupt, W; Zornig, C; Lacy, A

2013-05-01

The concept of natural orifice transluminal endoscopic surgery (NOTES) consists of the reduction of access trauma by using a natural orifice access to the intra-abdominal cavity. This could possibly lead to less postoperative pain, quicker recovery from surgery, fewer postoperative complications, fewer wound infections, and fewer long-term problems such as hernias. The Euro-NOTES Foundation has organized yearly meetings to work on this concept to bring it safely into clinical practice. The aim of this Euro-NOTES status update is to assess the yearly scientific working group reports and provide an overview on the current clinical practice of NOTES procedures. After the Euro-NOTES meeting 2011 in Frankfurt, Germany, an analysis was started regarding the most important topics of the European working groups. All prospectively documented information was gathered from Euro-NOTES and D-NOTES working groups from 2007 to 2011. The top five topics were analyzed. The statements of the working group activities demonstrate the growing information and changing insights. The most important selected topics were infection issue, peritoneal access, education and training, platforms and new technology, closure, suture, and anastomosis. The focus on research topics changed over time. The principle of hybrid access has overcome the technical and safety limitations of pure NOTES. Currently the following NOTES access routes are established for several indications: transvaginal access for cholecystectomy, appendectomy and colon resections; transesophageal access for myotomy; transgastric access for full-thickness small-tumor resections; and transanal/transcolonic access for rectal and colon resections. NOTES and hybrid NOTES techniques have emerged for all natural orifices and were introduced into clinical practice with a good safety record. There are different indications for different natural orifices. Each technique has been optimized for the purpose of finding a safe and realistic

Estabelecimento de protocolo para micropropagação de Lippia gracilis Schauer Establishment of protocol for Lippia gracilis Schauer micropropagation

Directory of Open Access Journals (Sweden)

M.J.M Marinho

2011-01-01

Full Text Available A espécie Lippia gracilis SCHAUER (Verbenaceae é nativa do Nordeste brasileiro e se destaca pela capacidade de acumular nos tricomas glandulares óleos essenciais com atividade antimicrobiana. Tendo em vista que não constam trabalhos na literatura sobre o estabelecimento in vitro dessa espécie, este trabalho teve como objetivo estabelecer protocolo para micropropagação de L. gracilis. Para tanto, ramos contendo folhas foram coletados de plantas matrizes no habitat natural para a confecção de estacas. Em laboratório, os explantes provenientes do processo de estaquia foram assepticamente tratados e inoculados em meio MS, acrescido de fitorreguladores, com o intuito de se estabelecer a melhor dose para o desenvolvimento dos explantes. Em decorrência de altos níveis de contaminação, avaliou-se o efeito da cefalexina. No entanto, apesar do antibiótico ter apresentado diminuição na contaminação bacteriana, a porcentagem de oxidação foi elevada. Portanto, testou-se o carvão ativado, ácido ascórbico, ácido cítrico e metade dos sais de MS quanto a eficiência no controle da oxidação. Concluiu-se que, o antibiótico na concentração utilizada provocou a oxidação dos explantes e os fitorreguladores, bem como os métodos antioxidantes testados, não apresentaram resultados consistentes para o melhor desenvolvimento dos explantes e controle da oxidação, respectivamente.The species Lippia gracilis Schauer (Verbenaceae is native to Northeastern Brazil and has been important for its ability to accumulate essential oils with antimicrobial activity in the glandular trichomes. Since there are no reports in the literature on the micropropagation of this species, the present work aimed to establish a protocol for L. gracilis micropropagation. Thus, branches containing leaves were collected from plant matrices in their natural habitat to prepare cuttings. In the laboratory, explants from cutting were aseptically treated and inoculated

Notes in Colombian Herpetology, II Notes in Colombian Herpetology, II

Directory of Open Access Journals (Sweden)

Dunn Emmett Reid

1944-03-01

Full Text Available The Lizard Genus Echinosaura (Teiidae in Colombia / Notes on the habits of the Tadpole-Carrying Frog Hyloxalus granuliventris / A New Marsupian Frog (Gastrotheca from Colombia The Lizard Genus Echinosaura (Teiidae in Colombia / Notes on the habits of the Tadpole-Carrying Frog Hyloxalus granuliventris / A New Marsupian Frog (Gastrotheca from Colombia.

Technology in Note Taking and Assessment: The Effects of Congruence on Student Performance

Directory of Open Access Journals (Sweden)

Matthew E. Barrett

2014-01-01

Full Text Available This study examined the encoding specificity principle in relation to traditional and computer-based note taking and assessment formats in higher education. Students (N = 79 took lecture notes either by hand (n = 40 or by computer (n = 39 and then completed either a computer or a paper-based assessment. When note taking and assessment formats were congruent, students scored significantly higher on the assessment when compared to students whose note taking and assessment format were incongruent. These findings highlight the importance of research on how in-class technology may affect student performance, and suggest that faculty and administrators seek to coordinate and standardize the use of assessment and note taking technologies where possible.

Note Taking for Geography Students.

Science.gov (United States)

Kneale, Pauline E.

1998-01-01

Addresses geography students' questions about why, when, and how to take notes. Outlines a step-by-step process for taking notes from written sources and from class lectures. Discusses what types of notes are appropriate for various types of sources. Suggests some ideas for making notes useful for individual learning styles. (DSK)

On that Note...

Science.gov (United States)

Stein, Harry

1988-01-01

Provides suggestions for note-taking from books, lectures, visual presentations, and laboratory experiments to enhance student knowledge, memory, and length of attention span during instruction. Describes topical and structural outlines, visual mapping, charting, three-column note-taking, and concept mapping. Benefits and application of…

European Science Notes. Volume 41, Number 9.

Science.gov (United States)

1987-09-01

management system and of the following: Secretaria de la Revista the inference engine. de Psicologia Social , Facultad de Psico- Application of Knowledge-Based...Notes NATO Advanced Research Workshop on Social and Environmental Psychology .................... William D. Crano 519 Applied Artificial Intelligence...An International Journal ................................. William D. Crano 520 New Spanish Journal of Social Psychology ................. William D

Needs analysis for developing a virtual-reality NOTES simulator.

Science.gov (United States)

Sankaranarayanan, Ganesh; Matthes, Kai; Nemani, Arun; Ahn, Woojin; Kato, Masayuki; Jones, Daniel B; Schwaitzberg, Steven; De, Suvranu

2013-05-01

INTRODUCTION AND STUDY AIM: Natural orifice translumenal endoscopic surgery (NOTES) is an emerging surgical technique that requires a cautious adoption approach to ensure patient safety. High-fidelity virtual-reality-based simulators allow development of new surgical procedures and tools and train medical personnel without risk to human patients. As part of a project funded by the National Institutes of Health, we are developing the virtual transluminal endoscopic surgery trainer (VTEST) for this purpose. The objective of this study is to conduct a structured needs analysis to identify the design parameters for such a virtual-reality-based simulator for NOTES. A 30-point questionnaire was distributed at the 2011 National Orifice Surgery Consortium for Assessment and Research meeting to obtain responses from experts. Ordinal logistic regression and the Wilcoxon rank-sum test were used for analysis. A total of 22 NOTES experts participated in the study. Cholecystectomy (CE, 68 %) followed by appendectomy (AE, 63 %) (CE vs AE, p = 0.0521) was selected as the first choice for simulation. Flexible (FL, 47 %) and hybrid (HY, 47 %) approaches were equally favorable compared with rigid (RI, 6 %) with p virtual NOTES simulator in training and testing new tools for NOTES were rated very high by the participants. Our study reinforces the importance of developing a virtual NOTES simulator and clearly presents expert preferences. The results of this analysis will direct our initial development of the VTEST platform.

BIOMASS ACCUMULATION AND NUTRITION IN MICROPROPAGATED PLANTS OF THE BANANA ‘PRATA CATARINA’ UNDER BIOFERTILISERS

Directory of Open Access Journals (Sweden)

EDER DE OLIVEIRA SANTOS

2017-01-01

Full Text Available Banana farming is an activity of great economic and social importance, and is carried out in most tropical countries. The aim of this work was to evaluate the biomass accumulation and levels of nitrogen (N, phosphorus (P, potassium (K, calcium (Ca and magnesium (Mg in micropropagated plants of the banana “Prata Catarina” during the acclimatization phase, under different types and doses of biofertilisers. The experimental design included randomised blocks in a 2 × 5 + (2 factorial scheme, with two types of liquid biofertilisers (bovine biofertiliser with anaerobic and aerobic fermentation and five biofertiliser doses (0.25, 0.50, 0.75, 1.00, and 1.25 L plant-1 week-1, as well as two additional treatments (control and recommended mineral fertilisation. The following variables were analysed: dry weight of the leaves and roots, and mineral element content (N, P, K, Ca, and Mg in different parts of the plant (leaf and root. During 90 days of acclimatization, the nutritional contribution of bovine biofertiliser with anaerobic fermentation was greater in comparison with the biofertiliser with aerobic fermentation and the control, but lower in comparison with mineral fertilisation. The 1000-mL dose of the biofertiliser with anaerobic fermentation promoted greater dry weight accumulation in the leaves and roots of the banana “Prata Catarina”. The biofertiliser with anaerobic fermentation promoted higher levels of N, K, and Ca in the leaves, whereas the biofertiliser with aerobic fermentation promoted higher levels of P in the leaves and roots.

VA OpenNotes: exploring the experiences of early patient adopters with access to clinical notes.

Science.gov (United States)

Nazi, Kim M; Turvey, Carolyn L; Klein, Dawn M; Hogan, Timothy P; Woods, Susan S

2015-03-01

To explore the experience of early patient adopters who accessed their clinical notes online using the Blue Button feature of the My HealtheVet portal. A web-based survey of VA patient portal users from June 22 to September 15, 2013. 33.5% of respondents knew that clinical notes could be viewed, and nearly one in four (23.5%) said that they had viewed their notes at least once. The majority of VA Notes users agreed that accessing their notes will help them to do a better job of taking medications as prescribed (80.1%) and be better prepared for clinic visits (88.6%). Nine out of 10 users agreed that use of visit notes will help them understand their conditions better (91.8%), and better remember the plan for their care (91.9%). In contrast, 87% disagreed that VA Notes will make them worry more, and 88.4% disagreed that access to VA Notes will be more confusing than helpful. Users who had either contacted their provider or healthcare team (11.9%) or planned to (13.5%) primarily wanted to learn more about a health issue, medication, or test results (53.7%). Initial assessment of the patient experience within the first 9 months of availability provides evidence that patients both value and benefit from online access to clinical notes. These findings are congruent with OpenNotes study findings on a broader scale. Additional outreach and education is needed to enhance patient awareness. Healthcare professionals should author notes keeping in mind the opportunity patient access presents for enhanced communication. © The Author 2014. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

The composer’s program note for newly-written classical music: content and intentions

Directory of Open Access Journals (Sweden)

Diana Mary Blom

2016-11-01

Full Text Available In concerts of western classical music the provision of a program note is a widespread practice dating back to the 18th century and still commonly in use. Program notes tend to inform listeners and performers about historical context, composer biographical details and compositional thinking. However, the scant program note research conducted to date reveals that program notes may not foster understanding or enhance listener enjoyment as previously assumed. In the case of canonic works, performers and listeners may already be familiar with much of the program note information. This is not so in the case of newly composed works, which formed the basis of the exploratory study reported here. This article reports the views of 17 living contemporary composers on their writing of program notes for their own works. In particular the study sought to understand the intended recipient, intended role and the content of composer-written program notes. Participating cComposers identified three main roles for their program notes: to shape a performer’s interpretation of the work; to guide, engage or direct the listener and/or performer; and as collaborative mode of communication between the composer, performer and listener. For some composers this collaboration was intended to result in performative listening in which listeners were actively engaged in bringing each composition to life. This was also described as a form of empathy that results in the co-construction of the musical experience. Overall, composers avoided giving too much personal information and they provided performers with more structural information. However, composers did not agree on whether the same information should be provided to both performers and listeners. Composers’ responses problematize the view of a program note as a simple statement from writer to recipient, indicating instead a more complex set of relations at play between composer, performer, listener and the work itself

Research Notes ~ Selecting Research Areas and Research Design Approaches in Distance Education: Process Issues

Directory of Open Access Journals (Sweden)

Sudarshan Mishra

2004-11-01

Full Text Available The purpose of this paper is to study the process used for selecting research areas and methodological approaches in distance education in India. Experts from the field of distance education in India were interviewed at length, with the aim of collecting qualitative data on opinions on process-issues for selecting areas for research, research design, and appropriate methodological approaches in distance education. Data collected from these interviews were subjected to content analysis; triangulation and peer consultation techniques were used for cross-checking and data verification. While the findings and recommendations of this study have limited application in that they can only be used in the specific context outlined in this paper, respondents in this study nonetheless revealed the pressing need for more process-oriented research in examining media and technology, learners and learning, and distance learning evaluation processes. Our research, which yielded interesting empirical findings, also determined that a mixed approach – one that involves both quantitative and qualitative methods – is more appropriate for conducting research in distance education in India. Qualitative evidence from our research also indicates that respondents interviewed felt that emphasis should be placed on interdisciplinary and systemic research, over that of traditional disciplinary research. Research methods such as student self-reporting, extensive and highly targeted interviews, conversation and discourse analysis, were determined to as useful for data collection for this study.

The Impact of Note Taking On the Improvement of Listening Comprehension of Iranian EFL Learners

OpenAIRE

Mohammad Zohrabi; Farzaneh Esfandyari

2014-01-01

Note taking is a popular and operative strategy which increases the students? ability to remember, comprehend, and keep the material in mind. Nowadays, it is very common for teachers to use the note taking strategy in EFL listening classes due to the fact that taking notes can help students catch the main points easily and in turn promote their listening comprehension effectively. However, it creates areas of concerns for some researchers about whether taking notes is effective for students t...

Lecture notes: string theory and zeta-function

Energy Technology Data Exchange (ETDEWEB)

Toppan, Francesco [Centro Brasileiro de Pesquisas Fisicas (CBPF), Rio de Janeiro, RJ (Brazil). E-mail: toppan@cbpf.br

2001-11-01

These lecture notes are based on a revised and LaTexed version of the Master thesis defended at ISAS. The research part being omitted, they included a review of the bosonic closed string a la Polyakov and of the one-loop background field method of quantisation defined through the zeta-function. In an appendix some basic features of the Riemann zeta-function are also reviewed. The pedagogical aspects of the material here presented are particularly emphasized. These notes are used, together with the Scherk's article in Rev. Mod. Phys. and the first volume of the Polchinski book, for the mini-course on String Theory (16-hours of lectures) held at CBPF. In this course the Green-Schwarz-Witten two-volumes book is also used for consultative purposes. (author)

Grouping Notes Through NodesThe Functions of Post-It™ Notes in Design Team Cognition

DEFF Research Database (Denmark)

Dove, Graham; Abildgaard, Sille Julie; Biskjaer, Michael Mose

The Post-It™ note is a frequently used, and yet seldom studied, design material. We investigate the functions Post-It™ notes serve when providing cognitive support for creative design team practice. Our investigation considers the ways in which Post-It™ notes function as design externalisations......, both individually and when grouped, and their role in categorisation in semantic long-term memory. To do this, we adopt a multimodal analytical approach focusing on interaction between humans, and between humans and artefacts, alongside language. We discuss in detail examples of four different...... externalisation functions served by Post-It™ notes, and show how these functions are present in complex overlapping combinations rather than being discrete. We then show how the temporal development of Post-It™ note interactions supports categorisation qualities of semantic long-term memory....

A Research Note on Time With Children in Different- and Same-Sex Two-Parent Families.

Science.gov (United States)

Prickett, Kate C; Martin-Storey, Alexa; Crosnoe, Robert

2015-06-01

Public debate on same-sex marriage often focuses on the disadvantages that children raised by same-sex couples may face. On one hand, little evidence suggests any difference in the outcomes of children raised by same-sex parents and different-sex parents. On the other hand, most studies are limited by problems of sample selection and size, and few directly measure the parenting practices thought to influence child development. This research note demonstrates how the 2003-2013 American Time Use Survey (n=44,188) may help to address these limitations. Two-tier Cragg's Tobit alternative models estimated the amount of time that parents in different-sex and same-sex couples engaged in child-focused time. Women in same-sex couples were more likely than either women or men in different-sex couples to spend such time with children. Overall, women (regardless of the gender of their partners) and men coupled with other men spent significantly more time with children than men coupled with women, conditional on spending any child-focused time. These results support prior research that different-sex couples do not invest in children at appreciably different levels than same-sex couples. We highlight the potential for existing nationally representative data sets to provide preliminary insights into the developmental experiences of children in nontraditional families.

SAFETY NOTES

CERN Document Server

TIS Secretariat

2001-01-01

Please note that the revisions of safety notes no 3 (NS 3 Rev. 2) and no 24 (NS 24 REV.) entitled respectively 'FIRE PREVENTION FOR ENCLOSED SPACES IN LARGE HALLS' and 'REMOVING UNBURIED ELV AND LVA ELECTRIC CONDUITS' are available on the web at the following urls: http://edmsoraweb.cern.ch:8001/cedar/doc.download?document_id=322811&version=1&filename=version_francaise.pdf http://edmsoraweb.cern.ch:8001/cedar/doc.download?document_id=322861&version=2&filename=version_francaise.pdf Paper copies can also be obtained from the TIS Divisional Secretariat, email tis.secretariat@cern.ch

and popular organization (research notes

Directory of Open Access Journals (Sweden)

Paulo J. Krischke

2006-01-01

Full Text Available This paper discusses some interfaces between the approaches to political learning, and their reference to situations of social exclusion, conflict and popular organization. The first part of the paper discusses the approach to the study of political learning among the elites; the second part examines approaches to research of political culture among the masses; and the third part outlines an alternative approach to political learning derived from Jürgen Habermas’s theory of “communicative action”. In the paper these approaches are applied to the study of a territory of exclusion, conflict, and popular organisation (a group of favelas in downtown Florianópolis, Santa Catarina, Brazil. Finally, the paper outlines some procedures to achieve a more general understanding of political learning

Integrator backstepping using contraction theory: a brief technological note

DEFF Research Database (Denmark)

Jouffroy, Jerome; Lottin, Jacques

While the use of Lyapunov function candidates for integrator backstepping has been extensively studied in the literature, little research has been conducted regarding the applicability of the so-called incremental stability approaches. This note addresses the problem of the use of an incremental ...

Efeito de substratos na aclimatização de mudas micropropagadas de abacaxizeiro cv. Pérola Acclimatization of micropropagated pineapple plants cv. "Pérola": substrata effect

Directory of Open Access Journals (Sweden)

Maria Aparecida Moreira

2006-10-01

Full Text Available Objetivou-se com este trabalho verificar o efeito da matéria orgânica como componente de substratos para mudas micropropagadas de abacaxizeiro cv. Pérola em fase de aclimatização. Mudas micropropagadas de abacaxizeiro cv. Pérola, selecionadas de acordo com o peso (aproximadamente 2 g, foram plantadas em bandejas de isopor de 72 células de 120 cm³ contendo proporções de substratos com terra, esterco bovino, Plantmax e matéria orgânica. O delineamento estatístico utilizado foi o inteiramente casualizado com 4 repetições e 3 plantas por parcela. As avaliações foram feitas 90 dias após o plantio, quando se avaliou: altura da planta, comprimento de raiz, peso de matéria fresca de raiz e parte aérea, peso de matéria seca de raiz e parte aérea e número de folhas. Com os resultados obtidos, conclui-se que a matéria orgânica tem efeito significativo no desenvolvimento das mudas. Com o substrato contendo terra, esterco e Plantmax foi obtido o melhor desenvolvimento das raízes e parte aérea.The objective of this work was to evaluate the effect of the organic matter as component of substrata for micropropagated plants of pineapple cv. Pérola in the acclimatization phase. Micropropagated plants were selected according to the weight (approximately 2g and planted in trays with 72 cels of 120 cm³, containing substrata made of soil, bovine manure, Plantmax and organic matter at different proportions. At 90 days after planting, the following parameters were evaluated: height of the plant, root length, weight of fresh matter of root and aerial part, dry matter weight of root and aerial part and number of leaves. The obtained results allowed to conclude that the organic matter has significant effect on the development of the seedlings. The best results for the development of the roots and aerial part were obtained with substratum containing soil, manure and Plantmax.

Natural orifice translumenal endoscopic surgery (NOTES) for innovation in hepatobiliary and pancreatic surgery: preface.

Science.gov (United States)

Sugimoto, Maki

2009-01-01

Natural orifice translumenal endoscopic surgery (NOTES) has captured the interest of interventional endoscopists and may represent the next stage of evolution of minimally invasive surgery. It provides the potential for performance of incisionless operations. It is gaining momentum both in the animal laboratory and in human case reports. Developments in the field of NOTES have led to the formation of the Natural Orifice Surgery Consortium for Assessment and Research (NOSCAR) in 2006. In this special issue, the current trends in NOTES in the field of hepatobiliary and pancreatic surgery are featured, including NOTES cholecystectomy, hepatectomy splenectomy, pancreatic necrosectomy, and the future of NOTES. In this issue, we discuss the potential benefits of these procedures in hepatobiliary and pancreatic surgery. We have just started the evaluation process for this new technology. The concept of NOTES is becoming established and is enormously advantageous for the patient. Both the surgeon and gastroenterologist should contribute to developing NOTES in making use of their specialties.

Employers' views on the fit note.

Science.gov (United States)

Kotze, E

2014-12-01

The fit note replaced the sick note in 2010. The statement of fitness for work (fit note) is expected to benefit the British economy by helping more people stay in work and prevent long-term sickness absence. Understanding and responding to employers' views on the fit note is key, in order for this goal to be achieved. To explore employers' views on the fit note. A qualitative study was undertaken and face-to-face interviews were conducted with participants representing employers from a variety of industries. There were 21 participants who were mainly human resources officers and line managers. Employers welcomed the introduction of the fit note and felt that it was an improvement on the sick note. The majority of employers felt the fit note had the potential to promote an earlier return to work, if used properly. The main problems reported were the completion of the fit notes and quality of advice received from general practitioners. Employers felt that the most helpful advice came from fit notes with information on the functional effects of the medical condition. Some employers found return to work decisions problematic. The fit note has the potential to promote an earlier return to work. In order for the fit note to achieve its aim, further understanding of the difficulties employers are having when making return to work decisions is important, in order to develop guidance to enable them to provide the practical support employees need to return to work sooner. © The Author 2014. Published by Oxford University Press on behalf of the Society of Occupational Medicine. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

EndNote 7.0

Directory of Open Access Journals (Sweden)

Eapen Bell

2006-01-01

Full Text Available EndNote is a useful software for online literature search and efficient bibliography management. It helps to format the bibliography according to the citation style of each journal. EndNote stores references in a library file, which can be shared with others. It can connect to online resources like PubMed and retrieve search results as per the search criteria. It can also effortlessly integrate with popular word processors like MS Word. The Indian Journal of Dermatology, Venereology and Leprology website has a provision to import references to EndNote.

The impact of note taking style and note availability at retrieval on mock jurors' recall and recognition of trial information.

Science.gov (United States)

Thorley, Craig; Baxter, Rebecca E; Lorek, Joanna

2016-01-01

Jurors forget critical trial information and what they do recall can be inaccurate. Jurors' recall of trial information can be enhanced by permitting them to take notes during a trial onto blank sheets of paper (henceforth called freestyle note taking). A recent innovation is the trial-ordered-notebook (TON) for jurors, which is a notebook containing headings outlining the trial proceedings and which has space beneath each heading for notes. In a direct comparison, TON note takers recalled more trial information than freestyle note takers. This study investigated whether or not note taking improves recall as a result of enhanced encoding or as a result of note access at retrieval. To assess this, mock jurors watched and freely recalled a trial video with one-fifth taking no notes, two-fifths taking freestyle notes and two-fifths using TONs. During retrieval, half of the freestyle and TON note takers could access their notes. Note taking enhanced recall, with the freestyle note takers and TON note takers without note access performing equally as well. Note taking therefore enhances encoding. Recall was greatest for the TON note takers with note access, suggesting a retrieval enhancement unique to this condition. The theoretical and applied implications of these findings are discussed.

Note Passing and Gendered Discipline in Vietnamese Schools

Science.gov (United States)

Horton, Paul

2015-01-01

While researchers agree that note passing is predominantly an activity engaged in by girls, there has been relatively little consideration of why this is the case. In this article, I argue that gendered expectations about the appropriate characters of boys and girls in Vietnam are incorporated into the disciplinary framework of schools, and that…

Notes toward a Philosophy of Action Learning Research

Science.gov (United States)

Coghlan, David; Coughlan, Paul

2010-01-01

The philosophical foundations of action learning research have not received a great deal of attention. In the context of action learning postgraduate and professional programmes in universities, articulation of a philosophy of action learning research seems timely and appropriate. This article explores a philosophy of action learning research,…

An Information Foraging Analysis of Note Taking and Note Sharing While Browsing Campaign Information

DEFF Research Database (Denmark)

Vatrapu, Ravi; Robertson, Scott

2010-01-01

In this paper, we present an experimental study of political information foraging in the context of e-voting. Participants were observed while searching and browsing the internet for campaign information in a mock-voting situation in three online note-taking conditions: No Notes, Private Notes...... with lack of scent, low value perception, and value depletion of information. Implications for the voter centered design of e-voting portals are discussed....

Notes on a methodological discussion: autobiography, critique and time

Directory of Open Access Journals (Sweden)

Gunvor Løkken

2010-06-01

Full Text Available After having tried for some time to overview the contemporary field of qualitative research to give a lecture for a professorship in that area, my idea at the outset of writing this article was to address whether changes in qualitative research should be viewed as recurrent revolutions as highlighted by Denzin and Lincoln (2000; 2005, or as a field of continuing key themes and long-standing tensions, as conceptualized by Atkinson, Coffey and Delamont (2003. However, during my writing, after one detour into the May 2009 issue of Current Sociology and a second detour into the July 2009 issue of Qualitative Research, my attention focused on to how critical debate and review are displayed in different methodological positions of qualitative research. In my reading, the discussion in Current Sociology between main stream and postmodern methodological positioning revealed an utterly one-way feminist critique; this was also the case in one of three book reviews of The Handbook of Qualitative Research (Denzin and Lincoln, 2005 in the referred issue of Qualitative Research. My puzzle over this critical stance, and my third detour, into Yvonne Lincoln’s discussion of twenty-five years of qualitative and new paradigm research in the January 2010 Issue of Qualitative Inquiry, helped evolve the following notes on a methodological discussion. The notes are partly structured by a temporal narrative over personally lived qualitative research, and partly by an epistemological narrative of a methodological discussion, interwoven with the passing of time when writing.

Effect of skill level on recall of visually presented patterns of musical notes.

Science.gov (United States)

Kalakoski, Virpi

2007-04-01

Expertise effects in music were studied in a new task: the construction of mental representations from separate fragments. Groups of expert musicians and non-musicians were asked to recall note patterns presented visually note by note. Skill-level, musical well-formedness of the note patterns and presentation mode were varied. The musicians recalled note patterns better than the non-musicians, even though the presentation was visual and successive. Furthermore, only musicians' performance was affected by musical well-formedness of the note patterns when visual gestalt properties, verbal rehearsability, and familiarity of the stimuli were controlled. Musicians were also able to use letter names referring to notes as efficiently as visual notes, which indicates that the better recall of musicians cannot be explained by perceptual visual chunking. These results and the effect of skill level on the distribution of recall errors indicate that the ability to chunk incoming information into meaningful units does not require that complete familiar patterns are accessible to encoding processes, yet previous knowledge stored in long-term memory affects representation construction in working memory. The present method offers a new reliable tool, and its implications to the research on construction of representations and musical imagery are discussed.

Understanding Digital Note-Taking Practice for Visualization.

Science.gov (United States)

Willett, Wesley; Goffin, Pascal; Isenberg, Petra

2015-05-13

We present results and design implications from a study of digital note-taking practice to examine how visualization can support revisitation, reflection, and collaboration around notes. As digital notebooks become common forms of external memory, keeping track of volumes of content is increasingly difficult. Information visualization tools can help give note-takers an overview of their content and allow them to explore diverse sets of notes, find and organize related content, and compare their notes with their collaborators. To ground the design of such tools, we conducted a detailed mixed-methods study of digital note-taking practice. We identify a variety of different editing, organization, and sharing methods used by digital note-takers, many of which result in notes becoming "lost in the pile''. These findings form the basis for our design considerations that examine how visualization can support the revisitation, organization, and sharing of digital notes.

Noted

Science.gov (United States)

Nunberg, Geoffrey

2013-01-01

Considering how much attention people lavish on the technologies of writing--scroll, codex, print, screen--it's striking how little they pay to the technologies for digesting and regurgitating it. One way or another, there's no sector of the modern world that is not saturated with note-taking--the bureaucracy, the liberal professions, the…

Making Notes, Making Meaning.

Science.gov (United States)

Burke, Jim

2002-01-01

Introduces notetaking tools used successfully with English-as-a-second-language students and low-achieving high school freshmen. Provides an overview of each tool and explains how students use them to take notes when reading textbooks and articles. Notes these tools and academic habits have helped students succeed in their mainstream academic…

A note on Fukui’s note

Directory of Open Access Journals (Sweden)

Tsai Cheng-Yu Edwin

2015-12-01

Full Text Available This commentary relates Fukui’s (2015 note on weak vs. strong generation to two aspects of quantification in Chinese: quantifier scope and the syntactic licensing conditions of noninterrogative wh-expressions. It is shown that the phenomena under discussion echo Fukui’s (2015 view that only strong generation allows for a deeper understanding of natural language and that dependencies are to be distinguished structurally.

RESEARCH NOTE Molecular genetic analysis of consanguineous ...

Indian Academy of Sciences (India)

Navya

Molecular genetic analysis of consanguineous families with primary microcephaly ... Translational Research Institute, Academic Health System, Hamad Medical ..... bridging the gap between homozygosity mapping and deep sequencing.

Notes on Piezoelectricity

Energy Technology Data Exchange (ETDEWEB)

Redondo, Antonio [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2016-02-03

These notes provide a pedagogical discussion of the physics of piezoelectricity. The exposition starts with a brief analysis of the classical (continuum) theory of piezoelectric phenomena in solids. The main subject of the notes is, however, a quantum mechanical analysis. We first derive the Frohlich Hamiltonian as part of the description of the electron-phonon interaction. The results of this analysis are then employed to derive the equations of piezoelectricity. A couple of examples with the zinc blende and and wurtzite structures are presented at the end

The new bank note distribution system

OpenAIRE

Gerrit Bilkes

1997-01-01

In this article, the author outlines the recent changes made to the way Canada's bank notes are distributed. The new system allows financial institutions to exchange notes directly with one another at designated points across the country, rather than through Bank of Canada agencies, as was previously the case. The institutions communicate with the Bank of Canada through a computerized inventory-management system. Two Bank of Canada operations centres monitor note quality and supply new notes ...

Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants.

Science.gov (United States)

Sarmast, Mostafa Khoshhal; Salehi, Hassan; Khosh-Khui, Morteza

2012-07-01

The objectives of the present work were in vitro propagation of Araucaria excelsa R. Br. var. glauca Carrière (Norfolk Island pine) with focus on the evaluation of the mean number of shoots per explant (MNS/E) and mean length of shoots per explants (MLS/E) produced by different parts of the orthotropic stem of A. excelsa R. Br. var. glauca in response to plant growth regulators. Norfolk Island pine axillary meristems responded very well to the 2-iso-pentenyl adenine (2iP) and thidiazuron (TDZ) levels. Explants taken from stem upper segments in the media containing 2iP had a higher MNS/E (3.47) and MLS/E (6.27 mm) in comparison to those taken from stem lower segments, which were 0.71 and 0.51 mm, respectively. Using 0.045 μM TDZ in the MS medium not only resulted in 4.60 MNS/E with 7.08 mm MLS/E but proliferated shoots showed a good performance as well. Investigating the best position of stem explant on mother plant as well as the best concentrations of growth regulators were performed which were useful for efficient micropropagation of this plant. Thirty three percent of explants were rooted in the MS medium containing 3 % sucrose, supplemented with 7.5 μM of both NAA and IBA for 2 weeks before transferring to a half strength MS medium without any growth regulator. Plantlets obtained were acclimatized and transferred to the greenhouse with less than 20 % mortality. This procedure considered the first successful report for regeneration and acclimatization of A. excelsa R. Br. var. glauca plantlet through main stem explants.

Adição de torta de mamona em substratos na aclimatação de mudas micropropagadas de bananeira Effect of addition of castor bean pie in substrates in acclimatization of micropropagated banana plantlets

Directory of Open Access Journals (Sweden)

Adriana Novais Martins

2011-03-01

Full Text Available A utilização de mudas micropropagadas de bananeira que oferecem qualidade genética e fitossanitária, favorecendo o desenvolvimento, instalação e uniformidade do pomar, é importante para a exploração comercial da bananicultura. O objetivo deste trabalho foi avaliar a influência da adição da torta de mamona nos substratos na aclimatação de mudas micropropagadas de bananeira da cv. Willians. O delineamento estatístico utilizado foi o inteiramente casualizado, com 10 tratamentos, sendo 2 substratos e 5 dosagens de torta de mamona (0; 6; 12; 18 e 24 g vaso-1. O substrato Vivatto Slim Plus® possibilitou o melhor desenvolvimento das plantas na aclimatação. Não são recomendadas doses superiores a 12 g planta-1 de torta de mamona misturadas ao substrato na aclimatação de mudas de bananeira.The use of micropropagated banana tree seedlings offer genetic and phytosanitary qualities, favoring the development, installation and uniformity of the yield and it is very important for the commercial exploration of banana fruits. The objective of this study was to evaluate the influence of castor oil plant pie added to the substrate in the acclimatization of micropropagated banana plantlets cv. 'Willians'. It was use the completely randomized, with 10 treatments, being 2 substrates and 5 dosages of castor oil plant pie (0; 6; 12; 18 and 24 g for each recipient. The substrate Vivatto Slim Plus® showed the best development of the plants in acclimatization processes. Dosages above 12 g of castor oil plant pie for each plant are not recommended mixed to the substrate in the acclimatization of banana tree seedlings

Project Notes

Science.gov (United States)

School Science Review, 1978

1978-01-01

Presents sixteen project notes developed by pupils of Chipping Norton School and Bristol Grammar School, in the United Kingdom. These Projects include eight biology A-level projects and eight Chemistry A-level projects. (HM)

European Science Notes. Volume 40, Number 1.

Science.gov (United States)

1986-01-01

Mass Spectrometry mers and copolymers of polyacrylate salt series edited by Professor J.F.J. Todd latex) rather than an inorganic or or- (University...changes in the popu- cy with two potassium dihydrogen phos- lation of a vibrational manifold were phate (KDP) crystals. Following a fil- determined by...AD-A162 235 EUROPEAN SCIENCE NOTES VOLUME 48 NUMBER I(U) OFFICE OF i/1 NAVAL RESEARCH LONDON (ENGLAND) L E SHAFFER JAN 86 UNCLASSIFIED F/G 5/2

Characteristics of suicide notes of children and young adolescents: an examination of the notes from suicide victims 15 years and younger.

Science.gov (United States)

Freuchen, Anne; Grøholt, Berit

2015-04-01

By analysing the suicide notes from suicide victims 15 years and younger, we attempt to gain insight into the process leading to youth suicide and explore the characteristics of the note writers. During a psychological autopsy on 42 youths, we were entrusted with 23 suicide notes in which we explored the themes and compared the note writers to the non-note writers. The key messages were as follows: explain the reason for suicide, declare love and give instructions. The note writers had showed suicidal behaviour more frequently than non-note writers. In the notes from youth suicide victims they present themselves as fully responsible and without confusion or overwhelming despair. The notes are likely equally informative as the notes of older victims. © The Author(s) 2013.

Note-Making in Social Studies.

Science.gov (United States)

Fowler, Robert W.

1985-01-01

Note-making is one excellent method for helping students retain important points made by the teacher. Techniques that elementary and secondary social studies teacher can use to teach note-making skills are described. (RM)

A Research Note on American Indian Criminal Justice

Science.gov (United States)

Braunstein, Rich; Anderson, Bill

2008-01-01

One confronts many difficulties when conducting policy-relevant criminal justice research that focuses on American Indian interests. Foremost among these difficulties is the great variation in relevant contexts that apply to this area of research. From the urban context of large American cities, where American Indians constitute a slim minority…

DigiMemo: Facilitating the Note Taking Process

Science.gov (United States)

Kurt, Serhat

2009-01-01

Everyone takes notes daily for various reasons. Note taking is very popular in school settings and generally recognized as an effective learning strategy. Further, note taking is a complex process because it requires understanding, selection of information and writing. Some new technological tools may facilitate the note taking process. Among such…

Editorial note

Directory of Open Access Journals (Sweden)

Tore Ahlbäck

2009-01-01

Full Text Available Editorial note of the Scripta Instituti Donneriani Aboensis, vol. 21, Postmodern Spirituality, based on papers read at the symposium on Postmodern Spirituality held at Åbo, Finland, on 11–13 June 2008.

Micropropagation of nucellar embryos and their histological comparative study for regeneration ability with other explants of kinnow mandarin (Citrus reticulata Blanco)

International Nuclear Information System (INIS)

Kazmi, S.K.; Khan, S.; Kabir, N.

2018-01-01

Polyembryony is the most beneficial and distinct character in citrus seeds. This characteristic can be beneficial in citrus improvement programs. Nucellar embryos developed from nucellar wall in citrus seeds along with zygotic embryo are found to have high plants regeneration ability in comparison to zygotic embryos. Under In vitro culture conditions, nucellar embryos were detected and multiplied on MT media with 0.5ml of coconut water along with 50g/L sucrose. These nucellar embryonic tissues were also regenerated on MT media upplemented with 0.5 mg/L BAP and 0.5 mg/L kinetin. Many shoots were regenerated from nucellar embryonic tissue; these shoots were rooted on MT media supplemented with IBA 0.5mg/L and NAA 0.1 mg/L in addition to activated charcoal 0.5gm/L. The resulting plantlets were acclimatized in the green house. After micropropagation, histological studies of nucellar embryonic tissues were carried out under fluorescence microscope to examine their high regeneration ability in comparison with usual plant parts, like seeds, shoots as well as with tissue culture stages including embryogenic and non-embryogenic callus. It was found that nucellar embryos have more regeneration ability as compared to usual plant parts and other tissue culture stages. (author)

RESEARCH NOTE

Indian Academy of Sciences (India)

decade of life, secondary to renal and cardiovascular complications or stroke. ... Genomic DNA was extracted from 10 mL of peripheral venous blood, collected in ... ischemia, cerebrovascular accidents, hypertension, dysrhythmias, and renal ...

Research note

DEFF Research Database (Denmark)

Cilliers, S C; Hayes, J P; Sales, J

1998-01-01

Apparent and true metabolisable energy (ME) values, corrected for zero nitrogen retention, of lucerne and barley were compared in balance studies between young (six months old, 50 to 60 kg BW) and mature (30 months old, 110 to 120 kg BW) ostriches. Birds were housed in individual metabolism crate...

Editor's Note Responding to suggestions from the research ...

African Journals Online (AJOL)

Admin

Responding to suggestions from the research fellowship of the Institute of African Studies for a re-branding of the. Research Review, which began publication in the early 1960s soon after the establishment of the Institute, the old title has now been replaced with a new title — Contemporary Journal of African Studies. This is ...

Note taking, review, memory, and comprehension.

Science.gov (United States)

Bohay, Mark; Blakely, Daniel P; Tamplin, Andrea K; Radvansky, Gabriel A

2011-01-01

In previous work assessing memory at various levels of representation, namely the surface form, textbase, and situation model levels, participants read texts but were otherwise not actively engaged with the texts. The current study tested the influence of active engagement with the material via note taking, along with the opportunity to review such notes, and the modality of presentation (text vs. spoken). The influence of these manipulations was assessed both immediately and 1 week later. In Experiment 1 participants read a text, whereas in Experiment 2 participants watched a video recording of the material being read as a lecture. For each experiment the opportunity to take notes was manipulated within participants, and the opportunity to review these notes before the test was manipulated between participants. Note taking improved performance at the situation model level in both experiments, although there was also some suggestion of benefit for the surface form. Thus, active engagement with material, such as note taking, appears to have the greatest benefit at the deeper levels of understanding.

Call for concept notes: Research on Myanmar's decentralization and ...

International Development Research Centre (IDRC) Digital Library (Canada)

... are launching this research call under a new initiative called Knowledge for ... Over the decades of military rule, information sharing, research, and freedom of ... Research outputs would take the form of working papers for peer-review and ...

Preceedings of the International Congress (12th), Corrosion Control for Low-Cost Reliability, Held in Houston, Texas on September 19 -24, 1993. Volume 3A. Corrosion: Specific Issues.

Science.gov (United States)

1993-09-24

corrosion fatigue damage accumulation. The density of microcracks, their influence on the stability of the passive layer, their ability to micropropagation ...software includes the nucleation of microcracks, their micropropagation and their coalescence at the specimen surface. At the beginning, a random number...surface micropropagation threshold (particularly to cross grain boundaries), the kinetics of propagation and then the crack velocity at the surface and

How Do High-Achieving Students Approach Web-Based, Copy and Paste Note Taking?: Selective Pasting and Related Learning Outcomes

Science.gov (United States)

Igo, L. Brent; Kiewra, Kenneth A.

2007-01-01

Previous research has indicated that most students copy and paste notes from Internet sources in a mindless way; they typically paste large sections of text into their notes and then later can recall little of what they have stored. However, supplying students with a note-taking framework that restricts the amount of text that may be pasted can…

Children's note taking as a mnemonic tool.

Science.gov (United States)

Eskritt, Michelle; McLeod, Kellie

2008-09-01

When given the opportunity to take notes in memory tasks, children sometimes make notes that are not useful. The current study examined the role that task constraints might play in the production of nonmnemonic notes. In Experiment 1, children played one easy and one difficult memory game twice, once with the opportunity to make notes and once without that opportunity. More children produced functional notations for the easier task than for the more difficult task, and their notations were beneficial to memory performance. Experiment 2 found that the majority of children who at first made nonmnemonic notations were able to produce functional notations with minimal training, and there was no significant difference in notation quality or memory performance between spontaneous and trained note takers. Experiment 3 revealed that the majority of children could transfer their training to a novel task. The results suggest that children's production of nonmnemonic notes may be due in part to a lack of knowledge regarding what task information is important to represent or how to represent it in their notes rather than to an inability to make functional notes in general.

Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process.

Science.gov (United States)

Duarte-Aké, Fátima; Castillo-Castro, Eduardo; Pool, Felipe Barredo; Espadas, Francisco; Santamaría, Jorge M; Robert, Manuel L; De-la-Peña, Clelia

2016-12-01

Global DNA methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in Agave angustifolia Haw. While the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. During the micropropagation of Agave angustifolia Haw., we found three different phenotypes, green (G), variegated (V) and albino (A). To understand the physiological and epigenetic differences among the somaclones, we analyzed several morphophysiological parameters and changes in the DNA methylation patterns in the three phenotypes during their in vitro development. We found that under in vitro conditions, the V plantlets maintained their CAM photosynthetic capacity, while the A variant showed no pigments and lost its CAM photosynthetic ability. Epigenetic analysis revealed that global DNA methylation increased in the G phenotype during the first two subcultures. However, after that time, DNA methylation levels declined. This hypomethylation correlated with the appearance of V shoots in the G plantlets. A similar correlation occurred in the V phenotype, where an increase of 2 % in the global DNA methylation levels was correlated with the generation of A shoots in the V plantlets. This suggests that an "epigenetic stress memory" during in vitro conditions causes a chromatin shift that favors the generation of variegated and albino shoots.

Mysore study: A study of suicide notes.

Science.gov (United States)

Namratha, P; Kishor, M; Sathyanarayana Rao, T S; Raman, Rajesh

2015-01-01

Suicide is one of the leading causes of preventable deaths. Recent data suggest South India as one of the regions with highest suicide rates in the world. In 2013, 134,799 people committed suicide in India according to the statistics released by the National Crime Records Bureau. Suicide note is one of the most important sources to understand suicide, which may be beneficial in suicide prevention. Studies on suicidal notes from this part of the world are sparse. The aim was to study the themes in suicide notes that might be useful in prevention strategies. A descriptive study of all suicide notes of those individuals who committed suicide between 2010 and 2013 available with Police Department, Mysore district was obtained and analyzed. A total of 22 suicide note were available. A majority of suicide note was in age group of 16-40 years (86%) and most were men (59%). All suicide notes were handwritten, the majority (70%) in regional language Kannada. Length of notes varied from just few words to few pages. Contents of suicide notes included apology/shame/guilt (80%), love for those left behind (55%) and instruction regarding practical affairs (23%). Most have blamed none for the act (50%). 23% mentioned that they are committing suicide to prove their innocence. 32% mentioned a last wish. The majority of suicidal note contained "guilt" which is a strong indicator of possible depression in deceased. Creating awareness about suicide among public and ensuring access to professionals trained in suicide prevention is need of the hour in this part of the world.

Tourism research and audio methods

DEFF Research Database (Denmark)

Jensen, Martin Trandberg

2016-01-01

• Audio methods enriches sensuous tourism ethnographies. • The note suggests five research avenues for future auditory scholarship. • Sensuous tourism research has neglected the role of sounds in embodied tourism experiences.......• Audio methods enriches sensuous tourism ethnographies. • The note suggests five research avenues for future auditory scholarship. • Sensuous tourism research has neglected the role of sounds in embodied tourism experiences....

Analytical dynamics course notes

CERN Document Server

Lindenbaum, Samuel D

1994-01-01

This book comprises a set of lecture notes on rational mechanics, for part of the graduate physics curriculum, delivered by the late Prof. Shirley L. Quimby during his tenure at Columbia University, New York. The notes contain proofs of basic theorems, derivations of formulae and amplification of observations, as well as the presentation and solution of illustrative problems. Collateral readings from more than 50 source references are indicated at appropriate places in the text.

Vertical specialization and industrial upgrading: a preliminary note

OpenAIRE

Xiao Jiang; William Milberg

2012-01-01

Abstract Vertical specialization is a measure of the import content of exports. Given the widely recognized importance of trade in tasks and global production networks, vertical specialization has recently gained the attention of international trade researchers and policy makers. In this note, we use measured changes in the within-country pattern of vertical specialization to gauge the relevance of task trade for industrial upgrading and economic development. We first calculate vertical speci...

Open notes: doctors and patients signing on.

Science.gov (United States)

Delbanco, Tom; Walker, Jan; Darer, Jonathan D; Elmore, Joann G; Feldman, Henry J; Leveille, Suzanne G; Ralston, James D; Ross, Stephen E; Vodicka, Elisabeth; Weber, Valerie D

2010-07-20

Few patients read their doctors' notes, despite having the legal right to do so. As information technology makes medical records more accessible and society calls for greater transparency, patients' interest in reading their doctors' notes may increase. Inviting patients to review these notes could improve understanding of their health, foster productive communication, stimulate shared decision making, and ultimately lead to better outcomes. Yet, easy access to doctors' notes could have negative consequences, such as confusing or worrying patients and complicating rather than improving patient-doctor communication. To gain evidence about the feasibility, benefits, and harms of providing patients ready access to electronic doctors' notes, a team of physicians and nurses have embarked on a demonstration and evaluation of a project called OpenNotes. The authors describe the intervention and share what they learned from conversations with doctors and patients during the planning stages. The team anticipates that "open notes" will spread and suggests that over time, if drafted collaboratively and signed by both doctors and patients, they might evolve to become contracts for care.

The Anatomy of a Note.

Science.gov (United States)

Moore, Herb

1986-01-01

Suggests that students can learn the physics of a musical note by learning how to synthesize sounds on a computer. Discusses ADSR (attack, decay, sustain, and release of a note) and includes a program (with listing) which students can use to examine ADSR on a Commodore 64 microcomputer. (JN)

Lecture Notes on Topics in Accelerator Physics

International Nuclear Information System (INIS)

Chao, Alex W.

2002-01-01

These are lecture notes that cover a selection of topics, some of them under current research, in accelerator physics. I try to derive the results from first principles, although the students are assumed to have an introductory knowledge of the basics. The topics covered are: (1) Panofsky-Wenzel and Planar Wake Theorems; (2) Echo Effect; (3) Crystalline Beam; (4) Fast Ion Instability; (5) Lawson-Woodward Theorem and Laser Acceleration in Free Space; (6) Spin Dynamics and Siberian Snakes; (7) Symplectic Approximation of Maps; (8) Truncated Power Series Algebra; and (9) Lie Algebra Technique for nonlinear Dynamics. The purpose of these lectures is not to elaborate, but to prepare the students so that they can do their own research. Each topic can be read independently of the others

Lecture Notes on Topics in Accelerator Physics

Energy Technology Data Exchange (ETDEWEB)

Chao, Alex W.

2002-11-15

These are lecture notes that cover a selection of topics, some of them under current research, in accelerator physics. I try to derive the results from first principles, although the students are assumed to have an introductory knowledge of the basics. The topics covered are: (1) Panofsky-Wenzel and Planar Wake Theorems; (2) Echo Effect; (3) Crystalline Beam; (4) Fast Ion Instability; (5) Lawson-Woodward Theorem and Laser Acceleration in Free Space; (6) Spin Dynamics and Siberian Snakes; (7) Symplectic Approximation of Maps; (8) Truncated Power Series Algebra; and (9) Lie Algebra Technique for nonlinear Dynamics. The purpose of these lectures is not to elaborate, but to prepare the students so that they can do their own research. Each topic can be read independently of the others.

Molecular gastronomy is a scientific discipline, and note by note cuisine is the next culinary trend

Directory of Open Access Journals (Sweden)

This Hervé

2013-01-01

Full Text Available Abstract For the past two decades, there has been much confusion about molecular gastronomy. This confusion has arisen because people ignore that the word gastronomy does not mean cuisine, it means knowledge about food. Similar to ‘molecular biology’, molecular gastronomy is a scientific discipline that looks for the mechanisms of phenomena occurring during dish preparation and consumption. As with any other scientific discipline, it can have many applications. One of the first was ‘molecular cuisine’ but since 1994, ‘note by note cuisine’ has also been promoted. The latter involves preparing dishes using pure compounds, or more practically mixture of compounds obtained by fractioning plant or animal tissues, instead of using these tissues themselves. Note by note cuisine raises issues in various fields: science, technology, nutrition, physiology, toxicology and politics.

Students' Note-Taking Challenges in the Twenty-First Century: Considerations for Teachers and Academic Staff Developers

Science.gov (United States)

van der Meer, Jacques

2012-01-01

Note-taking in lectures is often taken to be the distinguishing characteristic of learning at university. It is typically assumed that this is a commonsensical skill that students either have or will learn through trial and error. The data from a research project in one New Zealand university suggest that taking good notes is not a skill that…

Gaz de France. Operation note; Gaz de France. Note d'operation

Energy Technology Data Exchange (ETDEWEB)

NONE

2003-07-01

This note was published for the public at the occasion of the admission to Euronext's Eurolist of the existing shares that make the capital of Gaz de France company, the French gas utility. The note gives some informations about Gaz de France activity, and about its strategy of development in the European gas market. Then it describes the offer relative to the opening of Gaz de France capital. Some selected financial data and some precision about the risk factors and the management of the company complete the document. (J.S.)

How is adult patient adherence recorded in orthodontists’ clinical notes? A mixed-method case-note study

Directory of Open Access Journals (Sweden)

Al Shammary N

2017-10-01

Full Text Available Nawaf Al Shammary,1 Koula Asimakopoulou,1 Fraser McDonald,2 Jonathon T Newton,1 Sasha Scambler1 1Division of Population and Patient Health, 2Craniofacial Development and Stem Cell Biology Department, King’s College London Dental Institute, London, UKBackground: Patient adherence in orthodontic treatment is extremely important as it is linked with better treatment outcomes. Despite its importance, however, there is no shared definition of the concept. This makes the recording of adherence-related behaviors in patient notes difficult. The current study explored how, and to what extent adherence is recorded in adult patients’ medical records by orthodontists working in a large National Health Service (NHS London hospital.Materials and methods: A mixed-methods approach was used. A total of 17 clinicians with a mean age of 31 years (SD =4.87 provided N=20 case notes spanning N=324 appointments with patients they judged to be non-adherent. The notes were inspected for evidence of recording of patient adherence using adherence indicators identified in the literature.Results: The term “adherence” did not feature in any notes. The quantitative analysis showed that the three most frequent adherence-related behaviors recorded in notes were “oral hygiene,” “appointment attendance” and “breakages of appliances.” Qualitative analysis not only confirmed these factors but also showed that 1 the clinical aspects of treatment, 2 clinician–patient interaction factors and 3 patient attitudes also featured. This part of the analysis also highlighted inconsistencies across case notes in terms of the amount of information being recorded.Conclusion: Adherence as a term does not feature in the clinical case notes of clinician-identified non-adherent adult patients, while predictors of adherence are recorded with varying degrees of consistency. Keywords: adherence, adults, orthodontic treatment

Bank note recognition for the vision impaired.

Science.gov (United States)

Hinwood, A; Preston, P; Suaning, G J; Lovell, N H

2006-06-01

Blind Australians find great difficulty in recognising bank notes. Each note has the same feel, with no Braille markings, irregular edges or other tangible features. In Australia, there is only one device available that can assist blind people recognise their notes. Internationally, there are devices available; however they are expensive, complex and have not been developed to cater for Australian currency. This paper discusses a new device, the MoneyTalker that takes advantage of the largely different colours and patterns on each Australian bank note and recognises the notes electronically, using the reflection and transmission properties of light. Different coloured lights are transmitted through the inserted note and the corresponding sensors detect distinct ranges of values depending on the colour of the note. Various classification algorithms were studied and the final algorithm was chosen based on accuracy and speed of recognition. The MoneyTalker has shown an accuracy of more than 99%. A blind subject has tested the device and believes that it is usable, compact and affordable. Based on the devices that are available currently in Australia, the MoneyTalker is an effective alternative in terms of accuracy and usability.

Impact of Guided Notes on Achievement in K-12 and Special Education Students

Science.gov (United States)

Larwin, Karen H.; Dawson, Daniel; Erickson, Matthew; Larwin, David A.

2012-01-01

The common practice of using of guided notes in the K-12 and special education classroom is not fully appreciated or understood. In an effort to add to the existing research about this phenomenon, the current investigation expands on previously published research and one previously published meta-analysis that examined the impact of guided notes…

Note from TS/FM Group

CERN Document Server

2007-01-01

Renovation work at Restaurant no. 1 Please note that starting from 5 March and for one week, the men's toilets in Bldg. 500 (underground) will be closed because of the renovation work at Restaurant No. 1. The women's toilets in the same place will exceptionally be open to men and women during the same period. We apologize for the inconvenience and we are at your disposal for further information. Shuttle requests Please note that starting from 1 March 2007, shuttle requests: for official visits or bidders' conferences on the CERN site; to/from the airport or the centre of Geneva; for long distances, must be made via Fm.Support@cern.ch or by calling 77777. The radio taxi will still be reachable on 76969. Please note that starting from 1st of March, requests for the transport of PCs from your office to the PC store (Bldg. 513) and vice-versa, must be made the 'transport request' form on EDH under the field 'removals'). Mail distribution Please note that starting from 1 March 2007, mail distrib...

Technical Note

African Journals Online (AJOL)

Administratör

In this report on four patients, we did not use any of these techniques. The existence and the site of the fistulas was clearly demonstrated using basic but important preoperative detailed assessment and two intraoperative findings. The preoperative referral note that indicated the site of technical difficulty during the previous ...

Zebra Mussel Research Technical Notes. Impacts of Zebra Mussel Infestations on Water Quality. Section 1 - Environmental Testing

National Research Council Canada - National Science Library

Ashby, Steven

1998-01-01

..., and sediment quality. The purpose of this technical note describes potential changes in water quality as a result of zebra mussel infestations in aquatic systems, based on a review of the literature...

A note on axial symmetries

International Nuclear Information System (INIS)

Beetle, Christopher; Wilder, Shawn

2015-01-01

This note describes how to characterize and normalize an axial Killing field on a general Riemannian geometry or four-dimensional Lorentzian geometry. No global assumptions are necessary, such as that the orbits of the Killing field all have period 2π. Rather, any Killing field that vanishes at at least one point necessarily has the expected global properties. (note)

The Effectiveness of Note-Taking on Reading Comprehension of Iranian EFL Learners

Directory of Open Access Journals (Sweden)

Farnoush Bahrami

2017-10-01

Full Text Available By taking notes students could save time for reading all textbooks for their exams or for their representations. Taking notes increases attention of students to read or heard materials, and this increases their comprehension. Thus, the present study is important because note-taking could help them to remember what they learnt, absolutely important information. The method used in this research was survey. The 40 Persian EFL learners were selected from a language institute in Karaj to participate in the present study. These learners were divided into two groups; one of them is experimental group (N=20 and the other one is control group (N=20. Pretest and post test were two instruments that were used to carry out this study, a pretest about skill of note-taking of passages of the lessons was used for both experimental and control group. This test consisted of 4 passages. The same test was administrated again as the post test for both groups by the end of the course to see the different conclusion between taking note of experimental group and control group. Reliability between 4 texts is in oscillation from 0.6 to 0.81 (from 0.6 upwards. Therefore this reliability was an acceptable one. To analyze data descriptive statistics (that was contained percentage, frequency and mean score and also inferential statistics (that was contained ANOVA, Pearson correlation, independent sample t-test, multivariate’s test, regression were carried out by using SPSS16 soft ware. The findings confirmed that note taking is effective in reading comprehension.

What Predicts Skill in Lecture Note Taking?

Science.gov (United States)

Peverly, Stephen T.; Ramaswamy, Vivek; Brown, Cindy; Sumowski, James; Alidoost, Moona; Garner, Joanna

2007-01-01

Despite the importance of good lecture notes to test performance, very little is known about the cognitive processes that underlie effective lecture note taking. The primary purpose of the 2 studies reported (a pilot study and Study 1) was to investigate 3 processes hypothesized to be significantly related to quality of notes: transcription…

Editor's Note

African Journals Online (AJOL)

On another note: the editor and the editorial team acknowledge the financial support of the Carnegie Corporation of New York through the University of Ghana Building A New Generation of Academics in Africa (BANGA-Africa) Project. We also use this platform to express our gratitude for the support of various stakeholders, ...

Note-taking and Handouts in The Digital Age.

Science.gov (United States)

Stacy, Elizabeth Moore; Cain, Jeff

2015-09-25

Most educators consider note-taking a critical component of formal classroom learning. Advancements in technology such as tablet computers, mobile applications, and recorded lectures are altering classroom dynamics and affecting the way students compose and review class notes. These tools may improve a student's ability to take notes, but they also may hinder learning. In an era of dynamic technology developments, it is important for educators to routinely examine and evaluate influences on formal and informal learning environments. This paper discusses key background literature on student note-taking, identifies recent trends and potential implications of mobile technologies on classroom note-taking and student learning, and discusses future directions for note-taking in the context of digitally enabled lifelong learning.

Taking notes as an interactive process

OpenAIRE

Hornig, Wolfgang

1984-01-01

Taking notes as an interactive process : how to improve students´ notes / Hornig W. ; Nowak, J. - In: Nowak, Johann: Textverstehen und Textrekonstruktion in Vorlesungen. - Augsburg : HDZ, 1984. - S. 227-253. - (Augsburger Studien zur Hochschuldidaktik ; 12)

Notes to the Research on Political Socialisation in Hungary.

Science.gov (United States)

Keri, L.

1982-01-01

Hungarian research on political socialization is reviewed. The research is analyzed in terms of how the concept of political socialization is interpreted, the problems of interpreting political socialization, and the problems of judging the political socialization of youth. (AM)

[On the notes of the Xinkan Huangdimingtangjiujing (see text for symbol) in the possession of the National Diet Library].

Science.gov (United States)

Amano, Yosuke

2014-12-01

This paper analyzes the notes of Xinkan huangdimingtangjiujing which are in the possession of the National Diet Library, particularly referring to those notes which include "master said", or "master's theory". Some of those are found in the answers from Manase Dosan (see text for symbol) to Hata Soha (see text for symbol) collected in the Kotei meido kyukyo hushin shosho (see text for symbol) which is the record of the question and answer letters between Manase Dosan and Hata Soha. This research indicates that this volume has a close relation to the acupuncture research of Dosan's or Soha's school. And it indicates that the "master" in the Notes of Xinkan huangdimingtangjiujing refers to Dosan. Acupuncture research has been flourishing since the Edo era and has compiled a lot of volumes on acupuncture. This volume contains some pioneering pieces of this research done in the Azuchi Momoyama era by Dosan and Soha, and is a precious material, showing that some of the state of affairs of the research done in that age still remains today.

Grouping Notes Through Nodes

DEFF Research Database (Denmark)

Dove, Graham; Abildgaard, Sille Julie Jøhnk; Biskjær, Michael Mose

, both individually and when grouped, and their role in categorisation in semantic long-term memory. To do this, we adopt a multimodal analytical approach focusing on interaction between humans, and between humans and artefacts, alongside language. We discuss in detail examples of four different...... externalisation functions served by Post-ItTM notes, and show how these functions are present in complex overlapping combinations rather than being discrete. We then show how the temporal development of Post-ItTM note interactions supports categorisation qualities of semantic long-term memory....

Grouping Notes Through Nodes

DEFF Research Database (Denmark)

Dove, Graham; Abildgaard, Sille Julie; Biskjær, Michael Mose

2017-01-01

, both individually and when grouped, and their role in categorisation in semantic long-term memory. To do this, we adopt a multimodal analytical approach focusing on interaction between humans, and between humans and artefacts, alongside language. We discuss in detail examples of four different...... externalisation functions served by Post-ItTM notes, and show how these functions are present in complex overlapping combinations rather than being discrete. We then show how the temporal development of Post-ItTM note interactions supports categorisation qualities of semantic long-term memory....

A Pedagogic Cycle for EFL Note-Taking

Science.gov (United States)

Siegel, Joseph

2016-01-01

More attention has been given to note-taking in EFL classrooms in recent years, probably due to growing numbers of EFL students who attend lectures given in English and the importance of note-taking during language proficiency tests. While textbooks and materials for note-taking practice are readily available, classroom techniques for teaching EFL…

Quantum Optics and Nanophotonics : Lecture Notes of the Les Houches Summer School : Session CI

CERN Document Server

Sandoghdar, Vahid; Treps, Nicolas; Cugliandolo, Leticia F

2017-01-01

Quantum Optics and Nanophotonics consists of the lecture notes of the Les Houches Summer School 101 held in August 2013. Some of the most eminent experts in this flourishing area of research have contributed chapters lying at the intersection of basic quantum science and advanced nanotechnology. The book is part of the renowned series of tutorial books that contain the lecture notes of all the Les Houches Summer Schools since the 1950's and cover the latest developments in physics and related fields.

Debriefing Note Secondary Education Support Programme

DEFF Research Database (Denmark)

Webster, Neil; Vagnby, Bo Hellisen; Thomsen, Thomas J.

Debriefing note regarding joint programming for the Secondary Education Support Programme (2003- 2007). The note specifies preparation of SIP Physical Guidelines; Training needs assessment for Physical School Status and Rapid Technical Assessments; SIP/DEP preparation; Selection criteria...

Technical specifications (replaces note T.62). Irradiation of graphite at ambient temperature, Note T. 76; Specification technique, (Annule et remplace la note T. 62), Irradiation de graphite a temperature ambiante, Note T. 76

Energy Technology Data Exchange (ETDEWEB)

Reseau, R A [Services des grandes piles experimentales, Section ' Physique et Experimentation, Saclay (France)

1962-12-15

The objective is to study the effects of fast neutron irradiation of different graphite samples. The irradiation conditions should be as follows: integral fast neutron flux should be higher than 10{sup 20} neutrons/cm{sup 2}, the reactor should operate at steady state for 15 days, the temperature od samples should not be higher than 100 deg C, preferably 80 deg C. Note T. 62 which is replaced by this Note is attached.

News from the library: Lost in citations? Try EndNote Web

CERN Multimedia

CERN Library

2010-01-01

For a few months now, CERN users have had EndNote Web at their disposal, which comes together with access to the Web of Science online database. This bibliographic management tool will ease your work when collecting and organizing citations related to a research paper you are writing. It allows you to collect publication references from a wide variety of online sources via an online search, import text files or directly capture a bibliographic reference on a webpage with the Firefox EndNote plug-in.   You can store up to 10,000 references, which you can access from any computer and share with your colleagues. Thanks to this tool, you can format in one click your bibliography, according to the style of the journal you intend to submit your paper to. Last but not least, if you need to later export references already stored in EndNote Web, these can be saved in a standardized format, which makes them easy to manipulate and portable to other formats. The CERN Library will soon organize a training se...

15 CFR Notes Applicable to State... - Notes applicable to State of Understanding related to Medical Equipment:

Science.gov (United States)

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Notes applicable to State of Understanding related to Medical Equipment: applicable Notes applicable to State of Understanding related to Medical Equipment: Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY,...

Two-stage culture procedure using thidiazuron for efficient micropropagation of Stevia rebaudiana, an anti-diabetic medicinal herb.

Science.gov (United States)

Singh, Pallavi; Dwivedi, Padmanabh

2014-08-01

Stevia rebaudiana Bertoni, member of Asteraceae family, has bio-active compounds stevioside and rebaudioside which taste about 300 times sweeter than sucrose. It regulates blood sugar, prevents hypertension and tooth decay as well as used in treatment of skin disorders having high medicinal values, and hence there is a need for generating the plant on large scale. We have developed an efficient micropropagation protocol on half strength Murashige and Skoog (MS) media, using two-stage culture procedures. Varying concentrations of cytokinins, i.e., benzylaminopurine, kinetin and thidiazuron (TDZ) were supplemented in the nutrient media to observe their effects on shoot development. All the cytokinins promoted shoot formation, however, best response was observed in the TDZ (0.5 mg/l). The shoots from selected induction medium were sub-cultured on the multiplication media. The media containing 0.01 mg/l TDZ produced maximum number of shoot (11.00 ± 0.40) with longer shoots (7.17 ± 0.16) and highest number of leaves (61.00 ± 1.29). Rooting response was best observed in one-fourth strength on MS media supplemented with indole-3-butyric acid (1.0 mg/l) and activated charcoal (50 mg/l) with (11.00 ± 0.40) number of roots. The plantlets thus obtained were hardened and transferred to the pots with soil and sand mixture, where the survival rate was 80 % after 2 months. Quantitative analysis of stevioside content in leaves of in vivo mother plant and in vitro plantlets was carried out by high performance liquid chromatography. A remarkable increase in stevioside content was noticed in the in vitro-raised plants as compared to in vivo grown plants. The protocol reported here might be useful in genetic improvement and high stevioside production.

EFFICIENT MICROPROPAGATION FROM COTYLEDONARY NODE CULTURES OF COMMIPHORA WIGHTII (ARN. BHANDARI, AN ENDANGERED MEDICINALLY IMPORTANT DESERT PLANT

Directory of Open Access Journals (Sweden)

TARUN KANT

2010-12-01

Full Text Available Commiphora wightii (Arn. Bhandari, is a medicinal important desert species of the family Burseraceae. It is a well known for its valuable active principle found in its oleo-gum-resin (guggulsterone E and Z, which are used in drugs preparation for lowering the cholesterol level in human body. Due to its overexploitation, poor natural regeneration this valuable plant is on the verge of extinction and thus a IUCN Red listed species. In the present study we report development of an efficient micropropagation protocol from cotyledonary node of Commiphora wightii. Cotyledonary nodes were used as an explants and multiple microshoots were obtained on Murashige & Skoog (MS medium supplemented with 2.68 µM a-Naphthalene acetic acid (NAA and 4.44 µM 6-Benzylamino purine (BAP and on 2.68 µM NAA; 4.44 µM BAP with additives (glutamine 684.2 µM; thiamine 29.65 µM; activated charcoal 0.3% and various other hormonal combinations. Elongation of microshoot was significantly observed on the 2.46 µM Indole-3-butyric acid (IBA and 2.22 µM BAP supplemented MS medium. Efficient rooting was obtained on pretreated microshoot (4.92 µM IBA for 24 hours and followed by transfer to White’s medium without Plant Growth Regulators (PGR and high concentration of activated charcoal (AC. Rooted micro-shoots were transferred to vermiculite and wetted with Hoagland’s solution for primary hardening for 4-5 weeks and then finally transferred to plastic cups containing vermiculite, placed in mist chamber. Plantlets were transferred to soil: FYM 1:1 containing poly-bags, then to green shade house for complete acclimatization and finally transplanted to the experimental field.

Notes on Loricata (Mollusca) 11-14

NARCIS (Netherlands)

Kaas, P.

1985-01-01

Four new species of chitons (Mollusca, Polyplacophora) are described, viz Leptochiton (L.) dispersus and L. (L.) permodestus from Transkei, L. (L.) meiringae from the eastern Cape Province, S Africa (note 11) and Ischnochiton (Stenosemus) vanbellei from the Mediterranean Sea (note 14). New records

Writing a technical note.

Science.gov (United States)

Ng, K H; Peh, W C G

2010-02-01

A technical note is a short article giving a brief description of a specific development, technique or procedure, or it may describe a modification of an existing technique, procedure or device applicable to medicine. The technique, procedure or device described should have practical value and should contribute to clinical diagnosis or management. It could also present a software tool, or an experimental or computational method. Technical notes are variously referred to as technical innovations or technical developments. The main criteria for publication will be the novelty of concepts involved, the validity of the technique and its potential for clinical applications.

ESN information bulletin. European science notes information bulletin reports on current European/Middle eastern science

Energy Technology Data Exchange (ETDEWEB)

Orendorf, C.R.

1990-06-01

The European Science Notes Information Bulletin (ESNIB) 90-05 is a compilation of reports on recent developments in European science of specific interest to the U.S. research and development community, and is issued in support of the mission of the Office of Naval Research European Office. Issue Number 90-05, in addition to European area news, notes, and abstracts, contains reports in the fields of Acoustics, Atmospheric Electricity, Computer Science, Electronics, and Physics. The value of the ESNIB to Americans is to call attention to current activity in European science and technology and to identify the institutions and people responsible for these efforts. The ESNIB authors are primarily ONREUR staff members; other reports are prepared by or in cooperation with staff members of the USAF European Office of Aerospace Research and Development or the U.S. Army Research, Development and Standardination Group. Scientists from the U.S. who are traveling in Europe may also be invited to submit reports.

A Qualitative Analysis of How Online Access to Mental Health Notes Is Changing Clinician Perceptions of Power and the Therapeutic Relationship.

Science.gov (United States)

Denneson, Lauren M; Cromer, Risa; Williams, Holly B; Pisciotta, Maura; Dobscha, Steven K

2017-06-14

As part of the national OpenNotes initiative, the Veterans Health Administration (VHA) provides veterans online access to their clinical progress notes, raising concern in mental health settings. The aim of this study was to examine the perspectives and experiences of mental health clinicians with OpenNotes to better understand how OpenNotes may be affecting mental health care. We conducted individual semi-structured interviews with 28 VHA mental health clinicians and nurses. Transcripts were analyzed using a thematic analysis approach, which allows for both inductive and deductive themes to be explored using an iterative, constant comparative coding process. OpenNotes is changing VHA mental health care in ways that mental health clinicians perceive as both challenging and beneficial. At the heart of these changes is a shifting power distribution within the patient-clinician relationship. Some clinicians view OpenNotes as an opportunity to better partner with patients, whereas others feel that it has the potential to undo the therapeutic relationship. Many clinicians are uncomfortable with OpenNotes, but acknowledge that this discomfort could both improve and diminish care and documentation practices. Specifically, we found that (1) OpenNotes is empowering patients, (2) OpenNotes is affecting how clinicians build and maintain the therapeutic relationship, and (3) mental health clinicians are adjusting their practices to protect patients and themselves from adverse consequences of OpenNotes. Our findings suggest that future research should monitor whether OpenNotes notes facilitates stronger patient-clinician relationships, enhancing patient-centered mental health care, or diminishes the quality of mental health care through disruptions in the therapeutic relationship and reduced documentation. ©United States Government. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 14.06.2017.

Suicide Notes from India and the United States: A Thematic Comparison

Science.gov (United States)

Leenaars, Antoon A.; Girdhar, Shalina; Dogra, T. D.; Wenckstern, Susanne; Leenaars, Lindsey

2010-01-01

Suicide is a global concern, hence, cross-cultural research ought to be important; yet, there is a paucity of cross-cultural study in suicidology. This study sought to investigate suicide notes drawn from India and the United States, as these countries have similar suicide rates but markedly different cultures. A thematic or theoretical-conceptual…

SAFETY INSTRUCTION AND SAFETY NOTE

CERN Multimedia

TIS Secretariat

2002-01-01

Please note that the SAFETY INSTRUCTION N0 49 (IS 49) and the SAFETY NOTE N0 28 (NS 28) entitled respectively 'AVOIDING CHEMICAL POLLUTION OF WATER' and 'CERN EXHIBITIONS - FIRE PRECAUTIONS' are available on the web at the following urls: http://edms.cern.ch/document/335814 and http://edms.cern.ch/document/335861 Paper copies can also be obtained from the TIS Divisional Secretariat, email: TIS.Secretariat@cern.ch

Research notes : durability of composite repairs on bridges.

Science.gov (United States)

2009-08-01

The research showed that conditions that allow moisture to get under the carbon fiber reinforced polymer composites (CFRP) combined with freeze-thaw were detrimental to durability. In addition, the results showed that the American Concrete Institute ...

Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae

Directory of Open Access Journals (Sweden)

Virgínia F. Braga

2012-03-01

Full Text Available This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM/α-naphthaleneacetic acid (NAA; 0.005 µM induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM. Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg-1, which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis.Este trabalho descreve um protocolo eficiente de micropropagação para Verbena litoralis e estuda as atividades antinociceptiva e antioxidante de extratos desta espécie. Para o estabelecimento in vitro, os procedimentos de desinfecção e o PVPP mostraram alta eficiência no controle da contaminação por fungos e bactérias e da oxidação fenólica. O cultivo de segmentos nodais em meio MS suplementado com 6-benziladenina (7,5 µM e ácido α-naftalenoacético (ANA; 0,005 µM induziu múltiplos brotos. Brotos alongados foram enraizados com AIA (0,2 µM. As taxas de aclimatização foram elevadas e as plantas apresentaram características típicas da espécie. A fração hexânica (FH de folhas trituradas apresentou atividade sequestradora de radicais livres com IC50 = 169,3 µg mL-1. A FH mostrou atividade analgésica não dose-dependente no teste das contor

Protocolo de micropropagação da goiabeira serrana (Acca sellowiana (Berg Burret Feijoa (Acca sellowiana (Berg Burret micropropagation protocol

Directory of Open Access Journals (Sweden)

Ana Carla Oltramari

2000-03-01

Full Text Available Visando ao desenvolvimento de um novo protocolo para a micropropagação da goiabeira serrana (Acca sellowiana (Berg Burret foram estabelecidos experimentos com segmentos nodais e microestacas. As citocininas 6-Benzilaminopurina (BAP, Cinetina (Kin e 2-Isopenteniladenina (2-iP foram adicionadas ao meio de cultura Woody Plant Midium-WPM, visando à proliferação de brotações múltiplas dos genótipos 53B-7, 101, 529 e 152-12 x 458. As microestacas obtidas in vitro foram submetidas a diferentes períodos de indução, em 20 miM de ácido indolbutírico (AIB e, posteriormente, transferidas para meio de cultura isento desse fitorregulador para a indução de raízes ou, alternativamente, submetidas a diferentes concentrações e tempos de exposição em AIB e transferidas para substrato. As citocininas empregadas não promoveram aumento na taxa de proliferação de brotos em relação à testemunha. O meio de cultura basal WPM, adicionado de Kin (5 miM, proporcionou maior altura média dos brotos. Segmentos nodais do acesso 101 cultivados em meio de cultura WPM, isento de fitorreguladores, apresentaram maiores taxas médias de proliferação. Pulsos de seis dias com AIB (20 miM induziram uma maior taxa de enraizamento (68,9%, um maior número médio de raízes (1,3 raízes e raízes com maior comprimento médio (5,6miM. Microestacas enraizadas ex vitro, pela imersão em AIB (100 miM por 60 minutos, resultaram em maior altura das plantas (45,3mm, número de raízes secundárias (11,3 raízes, massa fresca (1069mg e seca das raízes (282mg.The objective of this study was to establish a micropropagation protocol for Acca sellowiana (Berg Burret. Nodal segments of in vitro cultivated plantlets of the genotypes 53B-7, 101, 529 and 152-12 x 458 were inoculated in test tubes containing 15ml of Woody Plant Medium-WPM solid medium supplemented with the cytokinins BAP, Kin, and 2-iP. For rooting purpose the microcuttings were submitted to different

Please note

CERN Multimedia

2006-01-01

Members of the personnel are invited to take note that only parcels corresponding to official orders or contracts will be handled at CERN. Individuals are not authorised to have private merchandise delivered to them at CERN and private deliveries will not be accepted by the Goods Reception services. Thank you for your understanding. (Version française la semaine prochaine.)

Notes on the Lasting Effects of Early Child Abuse throughout the Life Cycle.

Science.gov (United States)

Steele, Brandt F.

1986-01-01

Common themes in research on individuals who were abused as children are reviewed, noting transmission of a basic inability to provide good parenting. Psychological residues are discussed (including low self-esteem) along with delinquency, depression, attachment disorders, and sexual abuse. (CL)

Research Note: Headteacher Support Groups Initiative within the ...

African Journals Online (AJOL)

Eastern Africa Social Science Research Review. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 18, No 1 (2002) >. Log in or Register to get access to full text downloads.

Electronic health records improve clinical note quality.

Science.gov (United States)

Burke, Harry B; Sessums, Laura L; Hoang, Albert; Becher, Dorothy A; Fontelo, Paul; Liu, Fang; Stephens, Mark; Pangaro, Louis N; O'Malley, Patrick G; Baxi, Nancy S; Bunt, Christopher W; Capaldi, Vincent F; Chen, Julie M; Cooper, Barbara A; Djuric, David A; Hodge, Joshua A; Kane, Shawn; Magee, Charles; Makary, Zizette R; Mallory, Renee M; Miller, Thomas; Saperstein, Adam; Servey, Jessica; Gimbel, Ronald W

2015-01-01

The clinical note documents the clinician's information collection, problem assessment, clinical management, and its used for administrative purposes. Electronic health records (EHRs) are being implemented in clinical practices throughout the USA yet it is not known whether they improve the quality of clinical notes. The goal in this study was to determine if EHRs improve the quality of outpatient clinical notes. A five and a half year longitudinal retrospective multicenter quantitative study comparing the quality of handwritten and electronic outpatient clinical visit notes for 100 patients with type 2 diabetes at three time points: 6 months prior to the introduction of the EHR (before-EHR), 6 months after the introduction of the EHR (after-EHR), and 5 years after the introduction of the EHR (5-year-EHR). QNOTE, a validated quantitative instrument, was used to assess the quality of outpatient clinical notes. Its scores can range from a low of 0 to a high of 100. Sixteen primary care physicians with active practices used QNOTE to determine the quality of the 300 patient notes. The before-EHR, after-EHR, and 5-year-EHR grand mean scores (SD) were 52.0 (18.4), 61.2 (16.3), and 80.4 (8.9), respectively, and the change in scores for before-EHR to after-EHR and before-EHR to 5-year-EHR were 18% (pquality scores significantly improved over the 5-year time interval. The EHR significantly improved the overall quality of the outpatient clinical note and the quality of all its elements, including the core and non-core elements. To our knowledge, this is the first study to demonstrate that the EHR significantly improves the quality of clinical notes. © The Author 2014. Published by Oxford University Press on behalf of the American Medical Informatics Association.

Variation in chick-a-dee calls of tufted titmice, Baeolophus bicolor: note type and individual distinctiveness.

Science.gov (United States)

Owens, Jessica L; Freeberg, Todd M

2007-08-01

The chick-a-dee call of chickadee species (genus Poecile) has been the focus of much research. A great deal is known about the structural complexity and the meaning of variation in notes making up calls in these species. However, little is known about the likely homologous "chick-a-dee" call of the closely related tufted titmouse, Baeolophus bicolor. Tufted titmice are a prime candidate for comparative analyses of the call, because their vocal and social systems share many characteristics with those of chickadees. To address the paucity of data on the structure of chick-a-dee calls of tufted titmice, we recorded birds in field and aviary settings. Four main note types were identified in the call: Z, A, D(h), and D notes. Several acoustic parameters of each note type were measured, and statistical analyses revealed that the note types are acoustically distinct from one another. Furthermore, note types vary in the extent of individual distinctiveness reflected in their acoustic parameters. This first step towards understanding the chick-a-dee call of tufted titmice indicates that the call is comparable in structure and complexity to the calls of chickadees.

A Study of Search Intermediary Working Notes: Implications for IR System Design.

Science.gov (United States)

Spink, Amanda; Goodrum, Abby

1996-01-01

Reports findings from an exploratory study investigating working notes created during encoding and external storage (EES) processes by human search intermediaries (librarians at the University of North Texas) using a Boolean information retrieval (IR) system. Implications for the design of IR interfaces and further research is discussed.…

A note on tonal mobility in Chichewa | Moto | Journal of Humanities

African Journals Online (AJOL)

A note on tonal mobility in Chichewa. Francis Moto. Abstract. No Abstract. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's ...

Miniature surgical robots in the era of NOTES and LESS: dream or reality?

Science.gov (United States)

Zygomalas, Apollon; Kehagias, Ioannis; Giokas, Konstantinos; Koutsouris, Dimitrios

2015-02-01

Laparoscopy is an established method for the treatment of numerous surgical conditions. Natural orifice transluminal endoscopic surgery (NOTES) is a novel surgical technique that uses the natural orifices of the human body as entrances to the abdominal cavity. An alternative concept of minimally invasive approach to the abdominal cavity is to insert all the laparoscopic instruments through ports using a single small incision on the abdominal wall. A suggested name for this technique is laparoendoscopic single-site surgery (LESS). Considering the technical difficulties in NOTES and LESS and the progress in informatics and robotics, the use of robots seems ideal. The aim of this study is to investigate if there is at present, a realistic possibility of using miniature robots in NOTES or LESS in daily clinical practice. An up-to-date review on in vivo surgical miniature robots is made. A Web-based research of the English literature up to March 2013 using PubMed, Scopus, and Google Scholar as search engines was performed. The development of in vivo miniature robots for use in NOTES or LESS is a reality with great advancements, potential advantages, and possible application in minimally invasive surgery in the future. However, true totally NOTES or LESS procedures on humans using miniature robots either solely or as assistance, remain a dream at present. © The Author(s) 2014.

Stomatal and non-stomatal effects of exogenous abscisic acid during plant hardening

Czech Academy of Sciences Publication Activity Database

Pospíšilová, Jana; Synková, Helena; Haisel, Daniel; Baťková, Petra

2008-01-01

Roč. 133, - (2008), P09-131 ISSN 0031-9317 R&D Projects: GA ČR GA522/07/0227 Institutional research plan: CEZ:AV0Z50380511 Keywords : phytohormones * micropropagation * photosynthesis Subject RIV: ED - Physiology Impact factor: 2.334, year: 2008

RESEARCH NOTE THE PERFOR]\\IANCE DURING WINTER, OF ...

African Journals Online (AJOL)

THE PERFOR]\\IANCE DURING WINTER, OF HETFERS FED GRASS SILAGE, MADE UNDER. UNFAVOURABLE WEATHER CONDITIONS AND E. curvula HAY, PRODUCED. FROM THE SAME SWARD. Receipt of MS: 06-10-1981. A. van Niekerk. Cedara Agriculrural Research Station, PlBag X9059, Pietermaritzburg ...

Clinical Note Creation, Binning, and Artificial Intelligence

OpenAIRE

Deliberato, Rodrigo Oct?vio; Celi, Leo Anthony; Stone, David J

2017-01-01

The creation of medical notes in software applications poses an intrinsic problem in workflow as the technology inherently intervenes in the processes of collecting and assembling information, as well as the production of a data-driven note that meets both individual and healthcare system requirements. In addition, the note writing applications in currently available electronic health records (EHRs) do not function to support decision making to any substantial degree. We suggest that artifici...

Notes on the quantum theory of angular momentum

CERN Document Server

Feenberg, Eugene

1999-01-01

This classic, concise text has served a generation of physicists as an exceptionally useful guide to the mysteries of angular momenta and Clebsch-Gordon Coefficients. Derived from notes originally prepared to assist graduate students in reading research papers on atomic, molecular, and nuclear structure, the text first reviews the basic elements of quantum theory. It then examines the development of the fundamental commutation relations for angular momentum components and vector operators, and the ways in which matrix elements and eigenvalues of the angular momentum operators are worked out f

Observing professionals taking notes on screen

NARCIS (Netherlands)

Melenhorst, M.S.

2005-01-01

In this study 38 participants wrote a piece of advice based on reading and annotating information from an extensive Web site. Half of the participants took notes in a separate window, the other half used an advanced annotation tool. In text annotations were far more used than separate notes. The

Amusement Arcades Help Identify Teen Needs. Research Notes.

Science.gov (United States)

Jordan, Debra J.

1995-01-01

Reviews research on youth motivation for visiting amusement arcades and on the relationship among the school achievement, socioeconomic status, and self-esteem of fourth graders. Implications for camp involve providing adolescents with unstructured leisure time with little overt adult supervision and providing early intervention for low-achieving…

Technical note on drainage systems

DEFF Research Database (Denmark)

Bentzen, Thomas Ruby

This technical note will present simple but widely used methods for the design of drainage systems. The note will primarily deal with surface water (rainwater) which on a satisfactorily way should be transport into the drainage system. Traditional two types of sewer systems exist: A combined system......, where rainwater and sewage is transported in the same pipe, and a separate system where the two types of water are transported in individual pipe. This note will only focus on the separate rain/stormwater system, however, if domestic sewage should be included in the dimensioning procedure, it......’s not major different than described below - just remember to include this contribution for combined systems where the surface water (rain) and sewage are carried in the same pipes in the system and change some of the parameters for failure allowance (this will be elaborated further later on). The technical...

Using Guided Notes to Enhance Instruction for All Students

Science.gov (United States)

Konrad, Moira; Joseph, Laurice M.; Itoi, Madoka

2011-01-01

Taking notes from lectures or reading material can be challenging, especially for those who have learning disabilities. An alternative to traditional note-taking is a method called "guided notes," which has been found to improve the accuracy of students' notes, increase the frequency of student responses, and improve students' quiz and test…

Is testing a more effective learning strategy than note-taking?

Science.gov (United States)

Rummer, Ralf; Schweppe, Judith; Gerst, Kathleen; Wagner, Simon

2017-09-01

The testing effect is both robust and generalizable. However, most of the underlying studies compare testing to a rather ineffective control condition: massed repeated reading. This article therefore compares testing with note-taking, which has been shown to be more effective than repeated reading. Experiment 1 is based on a 3 × 3 between-participants design with the factors learning condition (repeated reading vs. repeated testing vs. repeated note-taking) and final test delay (5 min vs. 1 week vs. 2 weeks). It shows that in the immediate condition, learning performance is best after note-taking. After 1 week, both the note-taking and the testing groups outperform the rereading group, and after 2 weeks, testing is superior to both note-taking and rereading. Since repeated notetaking may not be the most effective (and common) operationalization of note-taking, Experiment 2 contrasts repeated testing with 2 other note-taking conditions: note-taking plus note-reading and note-taking plus testing (with only a 2-week final test delay). Both conditions that include a testing phase result in better long-term learning than note-taking plus note-reading. In summary, our findings indicate that-in the long run-testing is a powerful learning tool both in isolation and in combination with note-taking. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

Storytelling as Research/Research as Storytelling

Science.gov (United States)

Lewis, Patrick J.

2011-01-01

If story is central to human meaning why, in the research world, is there not more storytelling? Walter Benjamin (1973) noted that, "a story is different. It does not expend itself. It preserves and concentrates its strength and is capable of releasing it even after a long time" (p. 90). How might research not expend itself, but preserve and…

EVALUATION OF STUDENT'S NOTES IN A BLENDED LEARNING COURSE

OpenAIRE

Minoru Nakayama; Kouichi Mutsuura; Hiroh Yamamoto

2011-01-01

Student’s notes are evaluated to trace their learning process in a blended learning course, and the factors affecting the quality of these notes are discussed. As individual note-taking performance may be based on student’s characteristics, these contributions are also examined. Some factors about per-sonality and the learning experience are sig-nificant, and positively affect the grades given to notes. Lexical features of notes tak-en were extracted using a text analysis tech-nique, and ...

[Natural Orifice Transluminal Endoscopic Surgery (NOTES)].

Science.gov (United States)

Kim, Yong Sik; Kim, Chul Young; Chun, Hoon Jai

2008-03-01

Recently, the field of gastrointestinal endoscopy is developing rapidly. Once limited to the gastroinstestinal lumen, the endoscopic technology is now breaking the barriers and extending its boundary to peritoneal and pleural space. In 2004, Dr. Kalloo, a gastroenterologist, observed intraperitoneal organs of a pig using a conventional endoscope through the stomach wall. Since then, new endoscopic technique of intraperitoneal intervention with transluminal approach named the Natural Orifice Transluminal Endoscopic Surgery or NOTES has been introduced. NOTES reaches the target organ by inserting the endoscope through a natural orifice (e.g. mouth, anus, vagina, urethra) and entering the peritoneal lumen by means of making an incision on the luminal wall. After a series of successful experiences in animal studies, NOTES are now being tried on human subjects. There are still many obstacles to overcome, but bright future for this new technology is expected because of its proposed advantages of less pain, lower complication rate, short recovery time, and scarless access. In this review, we plan to learn about NOTES.

Physical chemistry research for engineering and applied sciences

CERN Document Server

Pearce, Eli M; Pethrick, Richard A; Zaikov, Gennady E

2015-01-01

PrefaceRegularity of Oxidation of Waste Fibrous and Film Materials of Polyethylene and Polypropylene: A Research Note; M. V. Bazunova, S. V. Kolesov, R. F. Tukhvatullin, E. I. Kulish, G. E. ZaikovA Research Note on Creation of ?arbon-Polymer Nanocomposites with Polyethylene as a Binder; Sergei V. Kolesov, Marina V. Bazunova, Elena I. Kulish, Denis R. Valiev, and Gennady E. ZaikovA Research Note on the Influence of Hybrid Antioxidants Ichphans on the Structure of Liposome Lipid Bilayer; E. Yu. Parshina, L. Ya. Gendel', and A. B. RubinDynamically Vulcanized Thermoelastoplastics Based on Butadien

PROPAGATION OF NEW BLACKBERRY CULTIVARS FOR PRODUCING CERTIFIED PROPAGATION MATERIAL

Directory of Open Access Journals (Sweden)

Valentina Isac

2014-12-01

Full Text Available The purpose of this research was to study the behaviour of two new Romanian thorn blackberry cultivars DAR 24 and DAR 8 in process of micropropagation, compared with Darrow cultivar usually commercially micropropagated in our laboratory. Due to several drawbacks of the conventional propagation of Rubus, the efficiency of micropropagation was tested in order to obtain high quality planting material and to introduce rapidly these new cultivars in a certification program. Thorn blackberry cultivars DAR 8 and DAR 24 with resistance to winter colds was successfully micropropagated. Blackberry plants were found without virus infection after biological and ELISA tests. Axillary buds from the branches in full growth were used as the initial explants. After four weeks of growth, aseptic cultures was established on MS basal mineral salts, LS vitamins with 0.3 mg/l BAP, 0.1mg/l GA3 and 0.001mg/l NAA. The rate of successfully established cultures was on average 65.11%. Good proliferation of the regenerated shoots was obtained on the same medium composition used for initiation phase, whereas medium MS with mineral salts reduced to ½ and LS vitamins with 0.1 mg/l IBA and 0.1 mg/l GA3 was used in the rooting phase. Dar 24 and Dar 8 cultivars responded by good rates of micropropagation on medium culture B as compared to control Darrow cultivar, even if the obtained shoots length was lower than on medium A. The statistical analysis reveled that the highest MR (20.66 plantlets/explant was obtained for Dar 24. In this case the length of shoots was 1.92 cm. The highest rooting percentages (over 85% were obtained with shoots multiplied on medium B. High quality of rooted plants induced a high percentage of acclimatization of cultivar Dar 8, 86.36% under mist system in green house whereas the percentage of acclimatization of cultivar Dar 24 was lower, 51.85%.

Evaluating the impact of patients' online access to doctors' visit notes: designing and executing the OpenNotes project

Directory of Open Access Journals (Sweden)

Leveille Suzanne G

2012-04-01

Full Text Available Abstract Background Providers and policymakers are pursuing strategies to increase patient engagement in health care. Increasingly, online sections of medical records are viewable by patients though seldom are clinicians' visit notes included. We designed a one-year multi-site trial of online patient accessible office visit notes, OpenNotes. We hypothesized that patients and primary care physicians (PCPs would want it to continue and that OpenNotes would not lead to significant disruptions to doctors' practices. Methods/Design Using a mixed methods approach, we designed a quasi-experimental study in 3 diverse healthcare systems in Boston, Pennsylvania, and Seattle. Two sites had existing patient internet portals; the third used an experimental portal. We targeted 3 key areas where we hypothesized the greatest impacts: beliefs and attitudes about OpenNotes, use of the patient internet portals, and patient-doctor communication. PCPs in the 3 sites were invited to participate in the intervention. Patients who were registered portal users of participating PCPs were given access to their PCPs' visit notes for one year. PCPs who declined participation in the intervention and their patients served as the comparison groups for the study. We applied the RE-AIM framework to our design in order to capture as comprehensive a picture as possible of the impact of OpenNotes. We developed pre- and post-intervention surveys for online administration addressing attitudes and experiences based on interviews and focus groups with patients and doctors. In addition, we tracked use of the internet portals before and during the intervention. Results PCP participation varied from 19% to 87% across the 3 sites; a total of 114 PCPs enrolled in the intervention with their 22,000 patients who were registered portal users. Approximately 40% of intervention and non-intervention patients at the 3 sites responded to the online survey, yielding a total of approximately 38

A study of actions in operative notes.

Science.gov (United States)

Wang, Yan; Pakhomov, Serguei; Burkart, Nora E; Ryan, James O; Melton, Genevieve B

2012-01-01

Operative notes contain rich information about techniques, instruments, and materials used in procedures. To assist development of effective information extraction (IE) techniques for operative notes, we investigated the sublanguage used to describe actions within the operative report 'procedure description' section. Deep parsing results of 362,310 operative notes with an expanded Stanford parser using the SPECIALIST Lexicon resulted in 200 verbs (92% coverage) including 147 action verbs. Nominal action predicates for each action verb were gathered from WordNet, SPECIALIST Lexicon, New Oxford American Dictionary and Stedman's Medical Dictionary. Coverage gaps were seen in existing lexical, domain, and semantic resources (Unified Medical Language System (UMLS) Metathesaurus, SPECIALIST Lexicon, WordNet and FrameNet). Our findings demonstrate the need to construct surgical domain-specific semantic resources for IE from operative notes.

Research note: Extracting a Cycvle from Ethiopian Agricultural ...

African Journals Online (AJOL)

This study has two objectives. First, to extract a cycle from agricultural GDP. Second, to compute the periodicity of the cycle. To achieve these objectives, a univariate time series procedure is applied. It is found that agricultural GDP is cyclic and that the cycle occurs every 8.25 years. (Eastern Africa Social Science Research ...

ESN information bulletin. European science notes information bulletin reports on current European/Middle eastern science

Energy Technology Data Exchange (ETDEWEB)

Orendorf, C.R.

1989-10-01

The European Science Notes Information Bulletin (ESNIB) 89-09 is a compilation of reports on recent developments in European science of specific interest to the U.S. research and development community, and is issued in support of the mission of the Office of Naval Research European Office. Issue Number 89-09, in addition to European area news, notes, and abstracts, contains reports in the fields of Acoustics, Computer Science, Condensed-Matter Physics, Materials Science, Mathematics, Physics, Psychology, and Solid-State Physics. It is not intended to be part of the scientific literature. The value of the ESNIB to Americans is to call attention to current activity in European science and technology and to identify the institutions and people responsible for these efforts. The ESNIB authors are primarily ONREUR staff members; other reports are prepared by or in cooperation with staff members of the USAF European Office of Aero space Research and Development or the U.S. Army Research, Development and Standardization Group. Scientists from the U.S. who are traveling in Europe may also be invited to submit reports.

Analyzing Language in Suicide Notes and Legacy Tokens.

Science.gov (United States)

Egnoto, Michael J; Griffin, Darrin J

2016-03-01

Identifying precursors that will aid in the discovery of individuals who may harm themselves or others has long been a focus of scholarly research. This work set out to determine if it is possible to use the legacy tokens of active shooters and notes left from individuals who completed suicide to uncover signals that foreshadow their behavior. A total of 25 suicide notes and 21 legacy tokens were compared with a sample of over 20,000 student writings for a preliminary computer-assisted text analysis to determine what differences can be coded with existing computer software to better identify students who may commit self-harm or harm to others. The results support that text analysis techniques with the Linguistic Inquiry and Word Count (LIWC) tool are effective for identifying suicidal or homicidal writings as distinct from each other and from a variety of student writings in an automated fashion. Findings indicate support for automated identification of writings that were associated with harm to self, harm to others, and various other student writing products. This work begins to uncover the viability or larger scale, low cost methods of automatic detection for individuals suffering from harmful ideation.

Non-targeted effects of ionising radiation (note). A new European integrated project, 2006-2010

International Nuclear Information System (INIS)

Salomaa, S.; Wright, E.G.; Hildebrandt, G.; Kadhim, M.; Little, M.P.; Prise, K.M.; Belyakov, O.V.

2007-01-01

Complete text of publication follows. The general objectives of the NOTE project are: (1) to investigate the mechanisms of nontargeted effects, in particular, bystander effects, genomic instability and adaptive response; (2) to investigate if and how non-targeted effects modulate the cancer risk in the low dose region, and whether they relate to protective or harmful functions; (3) to investigate if ionising radiation can cause non-cancer diseases or beneficial effects at low and intermediate doses; (4) to investigate individual susceptibility and other factors modifying non-targeted responses; (5) to assess the relevance of non-targeted effects for radiation protection and to set the scientific basis for a modern, more realistic, radiation safety system; (6) to contribute to the conceptualisation of a new paradigm in radiation biology that would cover both the classical direct (DNA-targeted) and non-targeted (indirect) effects. The NOTE brings together 19 major European and Canadian groups involved in the discovery, characterisation and mechanistic investigation of non-targeted effects of ionising radiation in cellular, tissue and animal models. The NOTE research activities are organised in six work packages. Four work packages (WPs 2-5) are problem-oriented, focussing on major questions relevant for the scientific basis of the system of radiation protection: WP2 Mechanisms of non-targeted effects, WP3 Non-cancer diseases, WP4 Factors modifying non-targeted responses, WP5 Modelling of non-targeted effects. The integration activities provided by WP6 strengthen the collaboration by supporting the access to infrastructures, mobility and training. WP7 provides dissemination and exploitation activities in the form of workshops and a public website. Managerial activities (WP1) ensure the organisation and structures for decision making, monitoring of progress, knowledge management and efficient flow of information and financing. Coordinator of the NOTE project is Prof

Gaz de France. Operation note

International Nuclear Information System (INIS)

2003-01-01

This note was published for the public at the occasion of the admission to Euronext's Eurolist of the existing shares that make the capital of Gaz de France company, the French gas utility. The note gives some informations about Gaz de France activity, and about its strategy of development in the European gas market. Then it describes the offer relative to the opening of Gaz de France capital. Some selected financial data and some precision about the risk factors and the management of the company complete the document. (J.S.)

Creation of an Accurate Algorithm to Detect Snellen Best Documented Visual Acuity from Ophthalmology Electronic Health Record Notes.

Science.gov (United States)

Mbagwu, Michael; French, Dustin D; Gill, Manjot; Mitchell, Christopher; Jackson, Kathryn; Kho, Abel; Bryar, Paul J

2016-05-04

Visual acuity is the primary measure used in ophthalmology to determine how well a patient can see. Visual acuity for a single eye may be recorded in multiple ways for a single patient visit (eg, Snellen vs. Jäger units vs. font print size), and be recorded for either distance or near vision. Capturing the best documented visual acuity (BDVA) of each eye in an individual patient visit is an important step for making electronic ophthalmology clinical notes useful in research. Currently, there is limited methodology for capturing BDVA in an efficient and accurate manner from electronic health record (EHR) notes. We developed an algorithm to detect BDVA for right and left eyes from defined fields within electronic ophthalmology clinical notes. We designed an algorithm to detect the BDVA from defined fields within 295,218 ophthalmology clinical notes with visual acuity data present. About 5668 unique responses were identified and an algorithm was developed to map all of the unique responses to a structured list of Snellen visual acuities. Visual acuity was captured from a total of 295,218 ophthalmology clinical notes during the study dates. The algorithm identified all visual acuities in the defined visual acuity section for each eye and returned a single BDVA for each eye. A clinician chart review of 100 random patient notes showed a 99% accuracy detecting BDVA from these records and 1% observed error. Our algorithm successfully captures best documented Snellen distance visual acuity from ophthalmology clinical notes and transforms a variety of inputs into a structured Snellen equivalent list. Our work, to the best of our knowledge, represents the first attempt at capturing visual acuity accurately from large numbers of electronic ophthalmology notes. Use of this algorithm can benefit research groups interested in assessing visual acuity for patient centered outcome. All codes used for this study are currently available, and will be made available online at https://phekb.org.

Utilizing Marzano's Summarizing and Note Taking Strategies on Seventh Grade Students' Mathematics Performance

Science.gov (United States)

Jeanmarie-Gardner, Charmaine

2013-01-01

A quasi-experimental research study was conducted that investigated the academic impact of utilizing Marzano's summarizing and note taking strategies on mathematic achievement. A sample of seventh graders from a middle school located on Long Island's North Shore was tested to determine whether significant differences existed in mathematic test…

Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins

Czech Academy of Sciences Publication Activity Database

Aremu, A.O.; Grúz, Jiří; Šubrtová, Michaela; Szüčová, Lucie; Doležal, Karel; Bairu, M. W.; Finnie, J. F.; van Staden, J.

2013-01-01

Roč. 170, č. 15 (2013), s. 1303-1308 ISSN 0176-1617 Grant - others:GA MŠk(CZ) ED0007/01/01 Program:ED Institutional research plan: CEZ:AV0Z50380511 Keywords : Conservation * Medicinal plants * Micropropagation Subject RIV: EF - Botanics Impact factor: 2.770, year: 2013

Intentional forgetting: note-taking as a naturalistic example.

Science.gov (United States)

Eskritt, Michelle; Ma, Sierra

2014-02-01

In the present study, we examined whether note-taking as a memory aid may provide a naturalistic example of intentional forgetting. In the first experiment, participants played Concentration, a memory card game in which the identity and location of pairs of cards need to be remembered. Before the game started, half of the participants were allowed to study the cards, and the other half made notes that were then unexpectedly taken away. No significant differences emerged between the two groups for remembering identity information, but the study group remembered significantly more location information than did the note-taking group. In a second experiment, we examined whether note-takers would show signs of proactive interference while playing Concentration repeatedly. The results indicated that they did not. The findings suggest that participants adopted an intentional-forgetting strategy when using notes to store certain types of information.

A hybrid approach to automatic de-identification of psychiatric notes.

Science.gov (United States)

Lee, Hee-Jin; Wu, Yonghui; Zhang, Yaoyun; Xu, Jun; Xu, Hua; Roberts, Kirk

2017-11-01

De-identification, or identifying and removing protected health information (PHI) from clinical data, is a critical step in making clinical data available for clinical applications and research. This paper presents a natural language processing system for automatic de-identification of psychiatric notes, which was designed to participate in the 2016 CEGS N-GRID shared task Track 1. The system has a hybrid structure that combines machine leaning techniques and rule-based approaches. The rule-based components exploit the structure of the psychiatric notes as well as characteristic surface patterns of PHI mentions. The machine learning components utilize supervised learning with rich features. In addition, the system performance was boosted with integration of additional data to the training set through domain adaptation. The hybrid system showed overall micro-averaged F-score 90.74 on the test set, second-best among all the participants of the CEGS N-GRID task. Copyright © 2017. Published by Elsevier Inc.

Application note :

Energy Technology Data Exchange (ETDEWEB)

Russo, Thomas V.

2013-08-01

The development of the XyceTM Parallel Electronic Simulator has focused entirely on the creation of a fast, scalable simulation tool, and has not included any schematic capture or data visualization tools. This application note will describe how to use the open source schematic capture tool gschem and its associated netlist creation tool gnetlist to create basic circuit designs for Xyce, and how to access advanced features of Xyce that are not directly supported by either gschem or gnetlist.

Interpreters' notes. On the choice of language

DEFF Research Database (Denmark)

Dam, Helle Vrønning

2004-01-01

This paper reports on a small-scale empirical study on note-taking in consecutive interpreting. As data, the study draws on the notes produced by four subjects while interpreting one Spanish source text consecutively into Danish, on the one hand, and one Danish source text into Spanish...... to particular scrutiny here. However, somewhat surprisingly, the results of the analyses indicate that the choice of language in note-taking is governed mainly by the status of the language in the interpreters' language combination, i.e. whether it is an A- or a B-language, and much less by its status......, on the other. The aim of the study is to explore what governs conference interpreters' choice of language for their notes. The categories traditionally used to discuss, describe and explain this choice are those of 'source language' and 'target language', and these categories are therefore subject...

NCEP Internal Office Notes

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The National Centers for Environmental Prediction (NCEP) and its predecessors have produced internal publications, known as Office Notes, since the mid-1950's. In...

Polyphonic Piano Transcription with a Note-Based Music Language Model

Directory of Open Access Journals (Sweden)

Qi Wang

2018-03-01

Full Text Available This paper proposes a note-based music language model (MLM for improving note-level polyphonic piano transcription. The MLM is based on the recurrent structure, which could model the temporal correlations between notes in music sequences. To combine the outputs of the note-based MLM and acoustic model directly, an integrated architecture is adopted in this paper. We also propose an inference algorithm, in which the note-based MLM is used to predict notes at the blank onsets in the thresholding transcription results. The experimental results show that the proposed inference algorithm improves the performance of note-level transcription. We also observe that the combination of the restricted Boltzmann machine (RBM and recurrent structure outperforms a single recurrent neural network (RNN or long short-term memory network (LSTM in modeling the high-dimensional note sequences. Among all the MLMs, LSTM-RBM helps the system yield the best results on all evaluation metrics regardless of the performance of acoustic models.

Online access to doctors' notes: patient concerns about privacy.

Science.gov (United States)

Vodicka, Elisabeth; Mejilla, Roanne; Leveille, Suzanne G; Ralston, James D; Darer, Jonathan D; Delbanco, Tom; Walker, Jan; Elmore, Joann G

2013-09-26

Offering patients online access to medical records, including doctors' visit notes, holds considerable potential to improve care. However, patients may worry about loss of privacy when accessing personal health information through Internet-based patient portals. The OpenNotes study provided patients at three US health care institutions with online access to their primary care doctors' notes and then collected survey data about their experiences, including their concerns about privacy before and after participation in the intervention. To identify patients' attitudes toward privacy when given electronic access to their medical records, including visit notes. The design used a nested cohort study of patients surveyed at baseline and after a 1-year period during which they were invited to read their visit notes through secure patient portals. Participants consisted of 3874 primary care patients from Beth Israel Deaconess Medical Center (Boston, MA), Geisinger Health System (Danville, PA), and Harborview Medical Center (Seattle, WA) who completed surveys before and after the OpenNotes intervention. The measures were patient-reported levels of concern regarding privacy associated with online access to visit notes. 32.91% of patients (1275/3874 respondents) reported concerns about privacy at baseline versus 36.63% (1419/3874 respondents) post-intervention. Baseline concerns were associated with non-white race/ethnicity and lower confidence in communicating with doctors, but were not associated with choosing to read notes or desire for continued online access post-intervention (nearly all patients with notes available chose to read them and wanted continued access). While the level of concern among most participants did not change during the intervention, 15.54% (602/3874 respondents, excluding participants who responded "don't know") reported more concern post-intervention, and 12.73% (493/3874 respondents, excluding participants who responded "don't know") reported less

Analysing playing using the note-time playing path.

Science.gov (United States)

de Graaff, Deborah L E; Schubert, Emery

2011-03-01

This article introduces a new method of data analysis that represents the playing of written music as a graph. The method, inspired by Miklaszewski, charts low-level note timings from a sound recording of a single-line instrument using high-precision audio-to-MIDI conversion software. Note onset times of pitch sequences are then plotted against the score-predicted timings to produce a Note-Time Playing Path (NTPP). The score-predicted onset time of each sequentially performed note (horizontal axis) unfolds in performed time down the page (vertical axis). NTPPs provide a visualisation that shows (1) tempo variations, (2) repetitive practice behaviours, (3) segmenting of material, (4) precise note time positions, and (5) time spent on playing or not playing. The NTPP can provide significant new insights into behaviour and cognition of music performance and may also be used to complement established traditional approaches such as think-alouds, interviews, and video coding.

Lecture note on neutron and photon transport calculation with MCNP

International Nuclear Information System (INIS)

Sakurai, Kiyoshi

2003-01-01

This paper is a lecture note on the continuous energy Monte Carlo method. The contents are as follows; history of the Monte Carlo study, continuous energy Monte Carlo codes, libraries, evaluation method for calculation results, integral emergent particle density equation, pseudorandom number, random walk, variance reduction techniques, MCNP weight window method, MCNP weight window generator, exponential transform, estimators, criticality problem and research subjects. This paper is a textbook for beginners on the Monte Carlo calculation. (author)

Europe Report, Science and Technology

Science.gov (United States)

1986-09-30

are to utilize tissue cultures mainly in the micropropagation of improved lines within the context of the maintenance cul- tivation of hybrid...types, micropropagation of cultivars and clones, mutation cultivation, the use of embryo cultures to overcome dormancy and resistance to cross

HOW TO ACHIEVE AND MAINTAIN NOTE 6: POSTGRADUATE PROGRAM IN TRANSLATIONAL SURGERY - UNIFESP

Directory of Open Access Journals (Sweden)

Miguel Sabino-Neto

Full Text Available Objective: To show the way to reach and stay in note 6 in the evaluation process of Medicine III of CAPES. Methods: Capes determinations were reviewed concerning this topic, grades 6 and 7, and also the difficulties and facilities of running a program that amounted to Note 6 after restructuring and being in compliance with regulations. Results: The main points to achieve and maintain Note 6 were: 1 regular production of master's and doctoral theses with appropriate distribution among all teachers; 2 average time of appropriate titration, as well as strict selection of students who resets the withdrawals and cancellations; 3 production of scientific articles in high impact journals and with academic and student participation in most part; 4 progressive and substantial increase in fundraising and patent search; 5 progressive increase in international exchanges with joint production; 6 visibility through new bilingual website and updated weekly; 7 numerous solidarity activities in research, but also in health services for the population and even in basic education; 8 rigorous selection of students (through design analysis, curriculum and teacher training program; 9 maintenance of high levels teachers production; 10 preparing new teachers for guidance through participation as co-supervision and involvement in the program to fit the needs. Conclusion: The Postgraduate Program in Translational Surgery went through difficult times; was submitted to a series of measures, adjustments, cooperation and understanding of the teaching staff, that took the program from note 3 - and almost closing - to a level of excellence keeping note 6 for three consecutive three-year periods of evaluation.

The Economics of Pricing and Customer Decision-Making. Research Notes.

Science.gov (United States)

Jordan, Debra J.

1996-01-01

Reviews two research articles. Gratton and Taylor, using economic analysis and market research, found that consumers of leisure experiences complained about price increases, but continued consuming. They recommend discounts for those in need and price increases focusing on service quality. Greenleaf and Lehmann identified 11 reasons why people…

Suicide Note Classification Using Natural Language Processing: A Content Analysis.

Science.gov (United States)

Pestian, John; Nasrallah, Henry; Matykiewicz, Pawel; Bennett, Aurora; Leenaars, Antoon

2010-08-04

Suicide is the second leading cause of death among 25-34 year olds and the third leading cause of death among 15-25 year olds in the United States. In the Emergency Department, where suicidal patients often present, estimating the risk of repeated attempts is generally left to clinical judgment. This paper presents our second attempt to determine the role of computational algorithms in understanding a suicidal patient's thoughts, as represented by suicide notes. We focus on developing methods of natural language processing that distinguish between genuine and elicited suicide notes. We hypothesize that machine learning algorithms can categorize suicide notes as well as mental health professionals and psychiatric physician trainees do. The data used are comprised of suicide notes from 33 suicide completers and matched to 33 elicited notes from healthy control group members. Eleven mental health professionals and 31 psychiatric trainees were asked to decide if a note was genuine or elicited. Their decisions were compared to nine different machine-learning algorithms. The results indicate that trainees accurately classified notes 49% of the time, mental health professionals accurately classified notes 63% of the time, and the best machine learning algorithm accurately classified the notes 78% of the time. This is an important step in developing an evidence-based predictor of repeated suicide attempts because it shows that natural language processing can aid in distinguishing between classes of suicidal notes.

Title: Effect of abiotic stress on reduction of microbial contamination ...

African Journals Online (AJOL)

TERI

2016-03-30

Mar 30, 2016 ... Full Length Research Paper. Effect of osmotic stress on in vitro propagation of. Musa sp. ... In vitro propagation of banana preferably use sword sucker as explant source where microbial contamination poses a great problem in ... micropropagation. Endo-bacterial contamination is one of the major problems ...

Sodium hypochlorite sterilization of culture medium in ...

African Journals Online (AJOL)

With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first ...

Note-taking and Handouts in The Digital Age

OpenAIRE

Stacy, Elizabeth Moore; Cain, Jeff

2015-01-01

Most educators consider note-taking a critical component of formal classroom learning. Advancements in technology such as tablet computers, mobile applications, and recorded lectures are altering classroom dynamics and affecting the way students compose and review class notes. These tools may improve a student’s ability to take notes, but they also may hinder learning. In an era of dynamic technology developments, it is important for educators to routinely examine and evaluate influences on f...

Note-taking Strategies and Academic Achievement

OpenAIRE

Haghverdi, Hamid; Biria, Reza; Karimi, Lotfollah

2014-01-01

The purpose of the present article is two fold. That is, revealing the significance, underlying theory and findings concerning note-taking in the literature related and exploring, through a survey study, the Iranian professors‘ and students‘ attitudes towards the effect of teaching note-taking strategies on the students‘ academic achievement. To this end, many previous studies were reviewed and we knew that many scholars, conducting empirical studies, surveys and interviews, have highlighted ...

Effect of zinc oxide (ZnO) nanoparticles on physiology and steviol glycosides production in micropropagated shoots of Stevia rebaudiana Bertoni.

Science.gov (United States)

Javed, Rabia; Usman, Muhammad; Yücesan, Buhara; Zia, Muhammad; Gürel, Ekrem

2017-01-01

This study aims to address the effects of different concentrations (0, 0.1, 1.0, 10, 100 or 1000 mg L -1 ) of engineered zinc oxide (ZnO) nanoparticles (34 nm in size) on growth parameters, steviol glycosides (rebaudioside A and stevioside) production and antioxidant activities in the tissue culture grown shoots of Stevia rebaudiana Bertoni. The highest percentage of shoot formation (89.6%) at 1 mg L -1 of ZnO nanoparticles concentration suggests a positive influence of ZnO nanoparticles on S. rebaudiana growth as compared to other treatments with or without ZnO nanoparticles. Additionally, HPLC results illustrate a significant enhancement of steviol glycosides (almost doubled as compared to the control) in micropropagated shoots grown under an oxidative stress of 1 mg L -1 of ZnO nanoparticles. This finding is further affirmed by an increased 2,2-diphenyl-1-picryl hydrazyl (DPPH) scavenging activity, total anti-oxidant capacity, total reducing power, total flavonoid content and total phenolic content, with an ascending oxidative pressure and generation of reactive oxygen species (ROS). However, the antioxidant activities, formation of secondary metabolites and the physiological parameters showed a sudden decline after crossing a threshold of 1 mg L -1 concentration of ZnO nanoparticles and falls to a minimum at 1000 mg L -1 , elucidating maximum phytotoxic effect of ZnO nanoparticles at this concentration. This is the first study evaluating both the favorable and adverse effects of ZnO nanoparticles employed to a highly valuable medicinal plant, S. rebaudiana. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Writing a Condolence Note

Science.gov (United States)

... through the eyes of others. For example, a mother whose son had died found out that her ... few suggestions on ending your condolence note: “Our love and support will always be here for you.” “ ...

In vitro clonal propagation of Mucuna pruriens var. utilis and its ...

African Journals Online (AJOL)

The genetic fidelity of the regenerated shoots was confirmed using randomly amplified polymorphic DNA (RAPD) analysis employing 15 operon primers. This system provides high fidelity micro-propagation system for efficient and rapid micro-propagation of this important green manure cover crop with medicinal properties.

Effects of calcium gluconate and ascorbic acid on controlling shoot ...

African Journals Online (AJOL)

In vitro shoot necrosis is a quite widespread disorder affecting raspberry micropropagation. This study was conducted to investigate effects of calcium gluconate and ascorbic acid on shoot necrosis and dieback of raspberry shoots during micropropagation. Nodal segments of primocane-fruiting raspberry cultivars 'Allgold', ...

Notes on the IMACON 500 streak camera system

International Nuclear Information System (INIS)

Clendenin, J.E.

1985-01-01

The notes provided are intended to supplement the instruction manual for the IMACON 500 streak camera system. The notes cover the streak analyzer, instructions for timing the streak camera, and calibration

Suicide Note Classification Using Natural Language Processing: A Content Analysis

Directory of Open Access Journals (Sweden)

John Pestian

2010-08-01

Full Text Available Suicide is the second leading cause of death among 25–34 year olds and the third leading cause of death among 15–25 year olds in the United States. In the Emergency Department, where suicidal patients often present, estimating the risk of repeated attempts is generally left to clinical judgment. This paper presents our second attempt to determine the role of computational algorithms in understanding a suicidal patient’s thoughts, as represented by suicide notes. We focus on developing methods of natural language processing that distinguish between genuine and elicited suicide notes. We hypothesize that machine learning algorithms can categorize suicide notes as well as mental health professionals and psychiatric physician trainees do. The data used are comprised of suicide notes from 33 suicide completers and matched to 33 elicited notes from healthy control group members. Eleven mental health professionals and 31 psychiatric trainees were asked to decide if a note was genuine or elicited. Their decisions were compared to nine different machine-learning algorithms. The results indicate that trainees accurately classified notes 49% of the time, mental health professionals accurately classified notes 63% of the time, and the best machine learning algorithm accurately classified the notes 78% of the time. This is an important step in developing an evidence-based predictor of repeated suicide attempts because it shows that natural language processing can aid in distinguishing between classes of suicidal notes.

Comparative analysis of public opinion research in the U.S. and Canada

Science.gov (United States)

Setlakwe, Linda; DiNunzio, Lisa A.

2004-06-01

Bank note producers are working to thwart the threat of counterfeit notes created using high resolution, digital image processing software and color output devices such as inkjet printers, color copiers, and scanners. Genuine notes must incorporate better overt and machine-readable security features that will reduce the chance of counterfeit notes being passed. Recently, Canada and the United States introduced newly designed bank notes that are intended to enable the general public to more easily distinguish genuine notes from counterfeits. The Bank of Canada (BoC) and the U.S. Department of Treasury"s Bureau of Engraving and Printing (BEP) have conducted similar market research projects to explore target audiences' perceptions and attitudes towards currency design and security features. This paper will present a comparative analysis of the two research projects, both of which were conducted using similar methodology. The results of these research studies assist in the selection of security features for future generations of bank notes.

The quality of operative notes at a general surgery unit.

Science.gov (United States)

Rogers, A; Bunting, M; Atherstone, A

2008-09-01

With the increasingly litigious nature of medical practice, accurate documentation is critical. This is particularly true for operative procedures, and medical councils have identified this and published guidelines to aid surgeons. However, these remain a frequently cited weakness in their defence in medico-legal cases. This study assessed the accuracy of operative notes in a general surgery unit in order to improve our practice. An audit of 100 consecutive operative notes was performed, and notes were assessed using the Royal College of Surgeons guidelines. The quality of note-taking of trainees was compared with that of consultant surgeons. A series of operation note pro formas was designed in response to the findings. Of the notes, 66% were completed by trainees. The vast majority of notes had no diagram to demonstrate the surgical findings or illustrate the actions. Specialist surgeons were more likely to describe the actions accurately, but less likely to describe wound closure methods or dressings used. They were also less likely to complete adequate postoperative orders. This study identifies key areas of weakness in our operative note-keeping. Pro formas should be introduced and made available for commonly performed procedures, and diagrams should be used wherever possible.

Sibling relationships of anxiety disordered children--a research note

NARCIS (Netherlands)

Lindhout, Ingeborg E.; Boer, Frits; Markus, Monica T.; Hoogendijk, Thea H. G.; Maingay, Ragna; Borst, Sophie R.

2003-01-01

There is a paucity of knowledge on the role of sibling relationships in internalizing disorders. Research in nonclinical populations suggests an association between internalizing problems in children and negative sibling interactions. Further, an association is reported between internalizing

Human Participants in Engineering Research: Notes from a Fledgling Ethics Committee.

Science.gov (United States)

Koepsell, David; Brinkman, Willem-Paul; Pont, Sylvia

2015-08-01

For the past half-century, issues relating to the ethical conduct of human research have focused largely on the domain of medical, and more recently social-psychological research. The modern regime of applied ethics, emerging as it has from the Nuremberg trials and certain other historical antecedents, applies the key principles of: autonomy, respect for persons, beneficence, non-maleficence, and justice to human beings who enter trials of experimental drugs and devices (Martensen in J Hist Med Allied Sci 56(2):168-175, 2001). Institutions such as Institutional Review Boards (in the U.S.) and Ethics Committees (in Europe and elsewhere) oversee most governmentally-funded medical research around the world, in more than a hundred nations that are signers of the Declaration of Helsinki (World Medical Association 2008). Increasingly, research outside of medicine has been recognized to pose potential risks to human subjects of experiments. Ethics committees now operate in the US, Canada, the U.K. and Australia to oversee all governmental-funded research, and in other jurisdictions, the range of research covered by such committees is expanding. Social science, anthropology, and other fields are falling under more clear directives to conduct a formal ethical review for basic research involving human participants (Federman et al. in Responsible research: a systems approach to protecting research participants. National Academies Press, Washington, 2003, p. 36). The legal and institutional response for protecting human subjects in the course of developing non-medical technologies, engineering, and design is currently vague, but some universities are establishing ethics committees to oversee their human subjects research even where the experiments involved are non-medical and not technically covered by the Declaration of Helsinki. In The Netherlands, as in most of Europe, Asia, Latin America, or Africa, no laws mandate an ethical review of non-medical research. Yet, nearly 2

A note on identification in discrete choice models with partial observability

DEFF Research Database (Denmark)

Fosgerau, Mogens; Ranjan, Abhishek

2017-01-01

This note establishes a new identification result for additive random utility discrete choice models. A decision-maker associates a random utility Uj+ mj to each alternative in a finite set j∈ {1 , … , J} , where U= {U1, … , UJ} is unobserved by the researcher and random with an unknown joint dis...... for applications where choices are observed aggregated into groups while prices and attributes vary at the level of individual alternatives....

Physiological responses and endogenous cytokinin profiles of tissue-cultured 'Williams' bananas in relation to roscovitine and an inhibitor of cytokinin oxidase/dehydrogenase (INCYDE) treatments

Czech Academy of Sciences Publication Activity Database

Aremu, A.O.; Bairu, M. W.; Novák, Ondřej; Plačková, Lenka; Zatloukal, Marek; Doležal, Karel; Finnie, J.F.; Strnad, Miroslav; van Staden, J.

2012-01-01

Roč. 236, č. 6 (2012), s. 1775-1790 ISSN 0032-0935 Grant - others:GA MŠk(CZ) ED0007/01/01 Program:ED Institutional research plan: CEZ:AV0Z50380511 Keywords : Cyclin-dependent kinase * Cytokinin metabolism * Micropropagation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.347, year: 2012

Research note : field control of asphalt concrete paving mixtures.

Science.gov (United States)

1995-01-01

The goal of this study was to develop information and evaluate new methods for controlling quality of the AC mixture in the mat. Specifically, this research project evaluated a gyratory compactor in the field laboratory to determine mix quality. Spec...

U-Note: Capture the Class and Access it Everywhere

DEFF Research Database (Denmark)

Malacria, Sylvain; Pietrzak, Thomas; Tabard, Aurélien

2011-01-01

We present U-Note, an augmented teaching and learning system leveraging the advantages of paper while letting teachers and pupils benefit from the richness that digital media can bring to a lecture. U-Note provides automatic linking between the notes of the pupils’ notebooks and various events...... on three modules. U-Teach captures the context of the class: audio recordings, the whiteboard contents, together with the web pages, videos and slideshows displayed during the lesson. U-Study binds pupils’ paper notes (taken with an Anoto digital pen) with the data coming from U-Teach and lets pupils...

MISCELLANEOUS BOTANICAL NOTES 2

Directory of Open Access Journals (Sweden)

A.J.G.H KOSTERMANS

2014-01-01

Full Text Available 1.   Durio  cupreus Ridley is considered to  represent a  distinct  species.2.   Durio wyatt-smithii Kosterm. is reported from Borneo.3.   Machilus nervosa Merr. represents Meliosma bontoeensis Merr.4.   Beilschmiedia brassii Allen represents Vavaea brassii (Allen Kosterm.5.   The author of the generic name Heritiera is Aiton.6.   Heritiera macrophylla (non Wall. Merr. is conspecific with H. ungus-tata Pierre.7.   Some specimens from N. Celebes, attributed formerly to H. sylvatica Merr., belong to H. arafurensis Kosterm.8.   Additional note on Heritiera littoralis Ait. and H. macrophylla Wall, ex Kurz.9.   Heritiera   montana   Kosterm.,   nov.   spec,   from   New   Guinea   and H. khidii Kosterm., nov. spec, from Northern Siam.10.   Additional note on Heritiera, novoguineensis Kosterm. and H. pereo-riacea Kosterm. and an undescribed species.11.   Heritiera acuminata Wall, ex Kurz represents a distinct species.12.   Heritiera  solomonensis  Kosterm.,  nov.  spec,  from the  Solomon  Isl.13.   A note on Firmiana bracteata A. DC.14.   Firmiana fulgens (Wall, ex King  Corner is based on a mixtum com-positum and has been the source of constant confusion. For the element, which occurs in Malaysia a new name is coined: F. malayana Kosterm. It does not occur in Tenasserim.15.   A revised bibliography of Firmiana colorata R. Br., F. pallens Stearn and F. malayana Kosterm. is presented.16.   Additional note on Firmiana hainanensis Kosterm.17.   Firmiana kerrii (Craib Kosterm., comb, nov., based on Sterculia kerrii Craib.18.   Additional specimens of Firmiana papuana Mildbr.19.   Cryptocarya hintonii Allen is referred to Primus as Primus hintonii (Allen  Kosterm.20.   Beilschmiedia wallichiana (G. Don   Kosterm., based on Sideroxylon wallichianum, G. Don, is described. Formerly it was relegated to Litsea by Kurz.21.   New species in Lauraceae: Beilschmiedia aborensis Kosterm., B

Justifications on social ineaqualities. Notes on the Salvadoran case

Directory of Open Access Journals (Sweden)

Irene Lungo Rodríguez

2016-05-01

Full Text Available This note presents the theoretical and methodological guidelines of my phd dissertation, which is under preparation. My concern is about cultural aspects that underlie social inequalities in contemporary El Salvador. Currently, this country is characterized by the primacy of neoliberal acumulation model, the rise of an unprecedented democracy, processes of reconcentration of capital and the persistence of high levels of social exclusion. Specifically, I study the justification of inequalities from the case of the “elite” middle classes in El Salvador. This research is inscribed whitin the sociological debates on the cultural aspects after reproduction of stratification and inequality.

Laparotomy operative note template constructed through a modified Delphi method.

Science.gov (United States)

Moore, Lolonya; Churley-Strom, Ruth; Singal, Bonita; O'Leary, Sharon

2009-05-01

An operative note is indispensable to physician documentation and decision-making; however, there are no accepted standards for operative note content. Our aim was to use a modified Delphi consensus-building method to construct a uniform operative note template for laparotomy. Using Joint Commission on Accreditation of Healthcare Organizations requirements, literature review, and feedback from 15 medical malpractice defense attorneys, we compiled a draft operative note template of 31 elements. We surveyed 37 Association of Professor of Gynecology and Obstetrics/Solvay scholars asking for their input on inclusion of each item as essential content of the operative note. Two iterations of the survey were required to reach a predetermined 75% level of consensus. Nine elements were eliminated from the template: 6 original and 3 expert-suggested elements. We provide an operative note template that was compiled through a Delphi process.

Improving operation notes to meet British Orthopaedic Association guidelines.

Science.gov (United States)

Morgan, David; Fisher, Noel; Ahmad, Aman; Alam, Fazle

2009-04-01

Operation notes are an important part of medical records for clinical, academic and medicolegal reasons. This study audited the quality of operative note keeping for total knee replacements against the standards set by the British Orthopaedic Association (BOA). A prospective review of all patients undergoing total knee replacement at a district general hospital over 8 months. Data recorded were compared with those required by the BOA good-practice guidelines. Change in practice was implemented and the audit cycle completed. Data were statistically analysed. A total of 129 operation notes were reviewed. There was a significant improvement in the mean number of data points recorded from 9.6 to 13.1. The least well recorded data were diagnosis, description of findings, alignment and postoperative flexion range. All had a significant improvement except description of findings. The operating surgeon writing the note improved from 56% to 67%. Detailed postoperative instructions also improved in quality. Surgeon education and the use of a checklist produce better quality total knee replacement operation notes in line with BOA guidelines. Further improvements may be made by making the data points part of the operation note itself.

QNOTE: an instrument for measuring the quality of EHR clinical notes.

Science.gov (United States)

Burke, Harry B; Hoang, Albert; Becher, Dorothy; Fontelo, Paul; Liu, Fang; Stephens, Mark; Pangaro, Louis N; Sessums, Laura L; O'Malley, Patrick; Baxi, Nancy S; Bunt, Christopher W; Capaldi, Vincent F; Chen, Julie M; Cooper, Barbara A; Djuric, David A; Hodge, Joshua A; Kane, Shawn; Magee, Charles; Makary, Zizette R; Mallory, Renee M; Miller, Thomas; Saperstein, Adam; Servey, Jessica; Gimbel, Ronald W

2014-01-01

The outpatient clinical note documents the clinician's information collection, problem assessment, and patient management, yet there is currently no validated instrument to measure the quality of the electronic clinical note. This study evaluated the validity of the QNOTE instrument, which assesses 12 elements in the clinical note, for measuring the quality of clinical notes. It also compared its performance with a global instrument that assesses the clinical note as a whole. Retrospective multicenter blinded study of the clinical notes of 100 outpatients with type 2 diabetes mellitus who had been seen in clinic on at least three occasions. The 300 notes were rated by eight general internal medicine and eight family medicine practicing physicians. The QNOTE instrument scored the quality of the note as the sum of a set of 12 note element scores, and its inter-rater agreement was measured by the intraclass correlation coefficient. The Global instrument scored the note in its entirety, and its inter-rater agreement was measured by the Fleiss κ. The overall QNOTE inter-rater agreement was 0.82 (CI 0.80 to 0.84), and its note quality score was 65 (CI 64 to 66). The Global inter-rater agreement was 0.24 (CI 0.19 to 0.29), and its note quality score was 52 (CI 49 to 55). The QNOTE quality scores were consistent, and the overall QNOTE score was significantly higher than the overall Global score (p=0.04). We found the QNOTE to be a valid instrument for evaluating the quality of electronic clinical notes, and its performance was superior to that of the Global instrument. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Report of second FAO/IAEA research coordination meeting on in vitro techniques for selection of radiation-induced mutants adapted to adverse environmental conditions. Working material

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-07-01

The meeting was attended by scientists from nine countries: Bangladesh, China P.R., Colombia, Egypt, Ghana, India, Pakistan, Peru and United States of America. The participants in this Research Coordination Meeting are working on the improvement of potato, sweet potato, garlic, sugarcane, pineapple and alfalfa by combining in vitro techniques with induced mutagenesis to select for resistance to salinity, freezing, heat, drought, and water-logging depending upon adverse condition prevailing in their region. The participants reported results of their experiments on the radio-sensitivity tests on in vitro cultured plant material, such as micropropagated plants and organogenic or embryogenic callus cultures. In addition, reports on the modifications of culture media required to regenerate and multiply local varieties and to carry out in vitro selection for specific stress conditions were presented. Refs, figs, tabs.

Report of second FAO/IAEA research coordination meeting on in vitro techniques for selection of radiation-induced mutants adapted to adverse environmental conditions. Working material

International Nuclear Information System (INIS)

1997-07-01

The meeting was attended by scientists from nine countries: Bangladesh, China P.R., Colombia, Egypt, Ghana, India, Pakistan, Peru and United States of America. The participants in this Research Coordination Meeting are working on the improvement of potato, sweet potato, garlic, sugarcane, pineapple and alfalfa by combining in vitro techniques with induced mutagenesis to select for resistance to salinity, freezing, heat, drought, and water-logging depending upon adverse condition prevailing in their region. The participants reported results of their experiments on the radio-sensitivity tests on in vitro cultured plant material, such as micropropagated plants and organogenic or embryogenic callus cultures. In addition, reports on the modifications of culture media required to regenerate and multiply local varieties and to carry out in vitro selection for specific stress conditions were presented. Refs, figs, tabs

Interpreters' notes. On the choice of language

DEFF Research Database (Denmark)

Dam, Helle Vrønning

2004-01-01

This paper reports on a small-scale empirical study on note-taking in consecutive interpreting. As data, the study draws on the notes produced by four subjects while interpreting one Spanish source text consecutively into Danish, on the one hand, and one Danish source text into Spanish, on the ot...... in the interpreting task, i.e. whether it functions as the source or the target language. Drawing on the concept of processing capacity and the Effort Model of consecutive, a tentative explanation of these findings is suggested......., on the other. The aim of the study is to explore what governs conference interpreters' choice of language for their notes. The categories traditionally used to discuss, describe and explain this choice are those of 'source language' and 'target language', and these categories are therefore subject...... to particular scrutiny here. However, somewhat surprisingly, the results of the analyses indicate that the choice of language in note-taking is governed mainly by the status of the language in the interpreters' language combination, i.e. whether it is an A- or a B-language, and much less by its status...

The Effects of Note-Taking Skills Instruction on Elementary Students' Reading

Science.gov (United States)

Chang, Wan-Chen; Ku, Yu-Min

2015-01-01

The authors investigated the effects of a 5-week note-taking skills instructional program on note-taking and reading comprehension performance of elementary students. The participants included 349 fourth-grade students from 2 elementary schools in Taiwan. The Note-Taking Instruction group received approximately 40 min of note-taking skills…

Euclidean distance can identify the mannitol level that produces the most remarkable integral effect on sugarcane micropropagation in temporary immersion bioreactors.

Science.gov (United States)

Gómez, Daviel; Hernández, L Ázaro; Yabor, Lourdes; Beemster, Gerrit T S; Tebbe, Christoph C; Papenbrock, Jutta; Lorenzo, José Carlos

2018-03-15

Plant scientists usually record several indicators in their abiotic factor experiments. The common statistical management involves univariate analyses. Such analyses generally create a split picture of the effects of experimental treatments since each indicator is addressed independently. The Euclidean distance combined with the information of the control treatment could have potential as an integrating indicator. The Euclidean distance has demonstrated its usefulness in many scientific fields but, as far as we know, it has not yet been employed for plant experimental analyses. To exemplify the use of the Euclidean distance in this field, we performed an experiment focused on the effects of mannitol on sugarcane micropropagation in temporary immersion bioreactors. Five mannitol concentrations were compared: 0, 50, 100, 150 and 200 mM. As dependent variables we recorded shoot multiplication rate, fresh weight, and levels of aldehydes, chlorophylls, carotenoids and phenolics. The statistical protocol which we then carried out integrated all dependent variables to easily identify the mannitol concentration that produced the most remarkable integral effect. Results provided by the Euclidean distance demonstrate a gradually increasing distance from the control in function of increasing mannitol concentrations. 200 mM mannitol caused the most significant alteration of sugarcane biochemistry and physiology under the experimental conditions described here. This treatment showed the longest statistically significant Euclidean distance to the control treatment (2.38). In contrast, 50 and 100 mM mannitol showed the lowest Euclidean distances (0.61 and 0.84, respectively) and thus poor integrated effects of mannitol. The analysis shown here indicates that the use of the Euclidean distance can contribute to establishing a more integrated evaluation of the contrasting mannitol treatments.

A NOTE ON THE POCHHAMMER FREQUENCY EQUATION

African Journals Online (AJOL)

cistvr

A note on the Pochhammer frequency equation. ),,,,(. ),,,,(;/. 2 zwura. ZWURA. tT ρω. µ. = ω= , where ωis the angular frequency of the wave, which is considered to be imposed in this problem. We also introduce a material parameter α defined by. )2. /(. µ+λµ=α , which is related to Poisson's ratio ν by n- n-. =a. 22. 21 . We note ...

Clinical Note Creation, Binning, and Artificial Intelligence.

Science.gov (United States)

Deliberato, Rodrigo Octávio; Celi, Leo Anthony; Stone, David J

2017-08-03

The creation of medical notes in software applications poses an intrinsic problem in workflow as the technology inherently intervenes in the processes of collecting and assembling information, as well as the production of a data-driven note that meets both individual and healthcare system requirements. In addition, the note writing applications in currently available electronic health records (EHRs) do not function to support decision making to any substantial degree. We suggest that artificial intelligence (AI) could be utilized to facilitate the workflows of the data collection and assembly processes, as well as to support the development of personalized, yet data-driven assessments and plans. ©Rodrigo Octávio Deliberato, Leo Anthony Celi, David J Stone. Originally published in JMIR Medical Informatics (http://medinform.jmir.org), 03.08.2017.

7 CFR 3550.208 - Reamortization using promissory note interest rate.

Science.gov (United States)

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Reamortization using promissory note interest rate... § 3550.208 Reamortization using promissory note interest rate. Reamortization using the promissory note interest rate may be authorized when RHS determines that reamortization is required to enable the borrower...

The concept of disclosure in the notes to financial statements

Directory of Open Access Journals (Sweden)

A.V. Ozeran

2015-12-01

Full Text Available The notes to Financial Statements are one of the most powerful sources of information for management decisions concerning a business entity. The tendency to overload informative notes causes rethinking of their role and content. The aim of the study is to discuss a number of ideas that set the requirements for disclosure in the notes to financial statements in order to prevent duplication of information in financial reporting as a whole and eliminate irrelevant disclosure to achieve clarity, consistency and effectiveness of the information contained in the notes. Based on the proposed clarified definition of «the notes to financial statements», we concluded that the notes should: a provide details and explanations of primary financial statements; b apply only to transactions and events existing at the reporting date; c focus on the needs of specific users and reflect reporting information specific to each entity. The development of the paper concepts will help strengthen the usefulness of company financial statements and increase their transparency.

Micropropagação do porta-enxerto de videira '420-A' Micropropagation of '420-A' grapevine rootstock

Directory of Open Access Journals (Sweden)

Pryscilla Menarin Dzazio

2002-12-01

meio MS/2 e aclimatização em substrato vermiculita ou Plantmax®.The objective of this work was to establish a protocol for the rootstock of 420-A micropropagation. The establishment of the cultures was accomplished with nodal segments, whose source of explants was the budding of woody stakes stored under refrigeration. In the initial cultivation were tested: the effect of 6-benzilaminopurine and kinetin with 0, 1, 5 and 10 µM concentrations, different culture medium (MS, NN and WPM and dilutions of the basic medium (MS, MS/2, MS/4 and MS/8. In the alongation and multiplication steps were tested the following culture medium: MS, MS/2, NN and WPM. In the rooting were tested: the MS/2 culture medium with or without activated coal (1gL-1. In the acclimatization were tested the substrate vermiculite, Plantmax and carbonized rice hulls. The kinetin didn't present effect on the budding and the growth of the nodal segments. BAP already promoted an increase in the number of sprouts per explant. The increase in the concentration of BAP reduced the number of leaves emitted by explant and increased the vitrification symptoms, being the best results obtained with 1 µM of BAP. In the initial cultivation the MS medium culture with the normal concentration of salts allowed the largest growth of the buddings. The dilutions of the MS medium in ¼ and 1/8 showed very harmful to the development of the rootstock '420-A', being quite harmed the growth of the shoots after the first subcultivation. During the multiplication the medium MS/2 showed more appropriate. The roots happened naturally during the multiplication, being unnecessary the use of activated coal in the culture medium. The acclimatization was accomplished with success in a misty camera, being obtained high survival rates in vermiculite (95,8% and Plantmax® (87%. It is concluded that the rootstock '420-A' can be micropropagated by initial cultivation of nodal segments in a culture medium of MS+1µM of BAP, multiplication

RESEARCH NOTE POST-WEANING BODY MASS GAIN OF FOUR ...

African Journals Online (AJOL)

POST-WEANING BODY MASS GAIN OF FOUR BREEDS OF STEERS ON DIFFERENT. NUTRITIONAL LEVELS. H.P. Eloff and C.J.F. Liidemann. Mara Research Station, Msa 0922. Receipt of MS. 4.8.1977. In South Africa there are 2O breeds of cattle which are mainly kept for the purpose of beef production (van. Marle ...

The Challenge of Interdisciplinary Research.

Science.gov (United States)

Locker, Kitty O.

1994-01-01

Discusses what makes business communication research interdisciplinary and why interdisciplinary research is difficult yet desirable. Details the value of interdisciplinary concepts, methods, and perspectives. Notes how business communication research might be made interdisciplinary and points out the need for tolerance in interdisciplinary…

Alcune Note di Analisi Matematica

OpenAIRE

Vasselli, Ezio

2011-01-01

Lectures notes (in italian) of some arguments of classical analysis, with exercises. A particular emphasis to functional analysis and elementary operator algebra theory is given, by means of exercises and examples.

Student notes as a mediating tool for learning in school subjects

DEFF Research Database (Denmark)

Christensen, Torben Spanget

2016-01-01

-writing function as a tool for a shift in identity from school child to student?. Perceptions of note-writing by two male students and students notes from the school subject bussines economics are analysed. It is concluded that note-writing can function as a tool for learning. Writing notes often allow students...... discource language which must be considered learning subjects. It is concluded that.note writing can mediate between subject discourse language and everyday language, and thereby also function as a tool to identity shift....

Note-Taking and Memory in Different Media Environments

Science.gov (United States)

Lin, Lin; Bigenho, Chris

2011-01-01

Through this study the authors investigated undergraduate students' memory recall in three media environments with three note-taking options, following an A x B design with nine experiments. The three environments included no-distraction, auditory-distraction, and auditory-visual-distraction; while the three note-taking options included…

Feature-Augmented Neural Networks for Patient Note De-identification

OpenAIRE

Lee, Ji Young; Dernoncourt, Franck; Uzuner, Ozlem; Szolovits, Peter

2016-01-01

Patient notes contain a wealth of information of potentially great interest to medical investigators. However, to protect patients' privacy, Protected Health Information (PHI) must be removed from the patient notes before they can be legally released, a process known as patient note de-identification. The main objective for a de-identification system is to have the highest possible recall. Recently, the first neural-network-based de-identification system has been proposed, yielding state-of-t...

Influence of different cytokinins used in in vitro culture on the stoma ...

African Journals Online (AJOL)

GREGO

2007-03-05

Mar 5, 2007 ... In vitro micropropagation of Pistachio (Pistacia vera var. Siirt), Plant Cell. Tissue Organ Cult. 77: 215–219. Pospisilova J, Ticha I, Kadlecek P, Haisel D, Plzakova S (1990a). Acclimatization of micropropagated plants to ex vitro conditions. Biologia Plantarum, 42: 481–497. Salarova J, Pospisilova J (1997).

Transgastric pure-NOTES peritoneoscopy and endoscopic ultrasonography for staging of gastrointestinal cancers

DEFF Research Database (Denmark)

Donatsky, Anders Meller; Vilmann, Peter; Meisner, Søren

2012-01-01

BACKGROUND: Human natural orifice transluminal endoscopic surgery (NOTES) has mainly been based on simultaneous laparoscopic assistance (hybrid NOTES), forgoing the theoretical benefits of the NOTES technique. This is due to a lack of NOTES-specific instruments and endoscopes, making pure-NOTES...... procedures difficult and time consuming. An area where pure NOTES could be adopted at its present stage of development is minimally invasive staging of gastrointestinal (GI) cancer. The aim of this study is to evaluate the feasibility of combining transgastric (TG) pure-NOTES peritoneoscopy...... and intraperitoneal endoscopic ultrasonography (ip-EUS) with intraluminal EUS (il-EUS) for peritoneal evaluation. METHODS: This was a feasibility and survival study where il-EUS followed by ip-EUS and peritoneoscopy was performed in 10 pigs subjected to TG pure NOTES. A score was given with regard to achieved...

Grouping notes through nodes: The functions of Post-It notes in design team cognition

DEFF Research Database (Denmark)

Dove, Graham; Abildgaard, Sille Julie Jøhnk; Biskjaer, Michael Mose

2018-01-01

We investigate the way Post-It notes support creative design team practice, focusing on how they function as cognitive externalisations that, through grouping activities, support categorisation qualities associated with semantic long-term memory. We use a multimodal approach, drawing...

Caracterização morfológica de cultivares de bananeira micropropagadas em estádio juvenil Morphological characterization of banana micropropagated cultivars in juvenile phase

Directory of Open Access Journals (Sweden)

Rafael Hansen Madail

2011-02-01

Full Text Available O objetivo deste trabalho foi determinar descritores morfológicos que possibilitassem a caracterização de cultivares de bananeira provenientes de micropropagação ainda na fase juvenil. Foram utilizadas 12 cultivares de bananeira de diferentes grupos genômicos e graus de ploidia, que foram cultivadas in vitro e posteriormente aclimatizadas em casa de vegetação. Após o período de três meses, foram avaliadas características morfológicas quantitativas e caracteres descritivos das plantas. Com base nos resultados obtidos, foi possível separar todas as cultivares estudadas e elaborar uma chave analítica que permite a identificação dessas cultivares após o período de apenas três meses de aclimatização.The aim of this study was to determine morphological descriptors that could enable the characterization of banana cultivars from micropropagation process during the juvenile phase. Twelve cultivars from different genomic groups and with different ploidy levels were grown in vitro and acclimatized in greenhouse for three months. After this period, plants quantitative morphological and descriptive characteristics were evaluated. The results allowed ranking the cultivars and preparing an analytical key to identify these cultivars after the period of three months of acclimatization.

Sarnet lecture notes on nuclear reactor severe accident phenomenology

International Nuclear Information System (INIS)

Trambauer, K.; Adroguer, B.; Fichot, F.; Muller, C.; Meyer, L.; Breitung, W.; Magallon, D.; Journeau, C.; Alsmeyer, H.; Housiadas, C.; Clement, B.; Ang, M.L.; Chaumont, B.; Ivanov, I.; Marguet, S.; Van Dorsselaere, J.P.; Fleurot, J.; Giordano, P.; Cranga, M.

2008-01-01

The 'Severe Accident Phenomenology Short Course' is part of the Excellence Spreading activities of the European Severe Accident Research NETwork of Excellence SARNET (project of the EURATOM 6. Framework programme). It was held at Cadarache, 9-13 January 2006. The course was divided in 14 lectures covering all aspects of severe accident phenomena that occur during a scenario. It also included lectures on PSA-2, Safety Assessment and design measures in new LWR plants for severe accident mitigation (SAM). This book presents the lecture notes of the Severe Accident Phenomenology Short Course and condenses the essential knowledge on severe accident phenomenology in 2008. (authors)

Note on the ABC Conjecture

OpenAIRE

Carella, N. A.

2006-01-01

This note imparts heuristic arguments and theorectical evidences that contradict the abc conjecture over the rational numbers. In addition, the rudimentary datails for transforming this problem into the doimain of equidistribution theory are provided.

Lecture Notes in Statistics. 3rd Semester

DEFF Research Database (Denmark)

The lecture note is prepared to meet the requirements for the 3rd semester course in statistics at the Aarhus School of Business. It focuses on multiple regression models, analysis of variance, and log-linear models.......The lecture note is prepared to meet the requirements for the 3rd semester course in statistics at the Aarhus School of Business. It focuses on multiple regression models, analysis of variance, and log-linear models....

Note-Taking with Computers: Exploring Alternative Strategies for Improved Recall

Science.gov (United States)

Bui, Dung C.; Myerson, Joel; Hale, Sandra

2013-01-01

Three experiments examined note-taking strategies and their relation to recall. In Experiment 1, participants were instructed either to take organized lecture notes or to try and transcribe the lecture, and they either took their notes by hand or typed them into a computer. Those instructed to transcribe the lecture using a computer showed the…

The Impact of Novice Counselors' Note-Taking Behavior on Recall and Judgment

Science.gov (United States)

Lo, Chu-Ling; Wadsworth, John

2014-01-01

Purpose: To examine the effect of note-taking on novice counselors' recall and judgment of interview information in four situations: no notes, taking notes, taking notes and reviewing these notes, and reviewing notes taken by others. Method: The sample included 13 counselors-in-training recruited from a master's level training program in…

Investigation of Endophytic Bacterial Community in Supposedly Axenic Cultures of Pineapple and Orchids with Evidence on Abundant Intracellular Bacteria.

Science.gov (United States)

Esposito-Polesi, Natalia Pimentel; de Abreu-Tarazi, Monita Fiori; de Almeida, Cristina Vieira; Tsai, Siu Mui; de Almeida, Marcílio

2017-01-01

Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year. Isolation and characterization of bacterial isolates, PCR-DGGE from total genomic DNA of microplants and ultrastructural analysis of leaves were performed. In the culture-dependent technique, it was only possible to obtain bacterial isolates from pineapple microplants. In this case, the bacteria genera identified in the isolation technique were Bacillus, Acinetobacter, and Methylobacterium. The scanning electron microscopy and transmission electron microscopy (SEM and TEM) analyses revealed the presence of endophytic bacteria in intracellular spaces in the leaves of pineapple and orchid microplants, independent of the laboratory or cultivation protocol. Our results strongly indicate that there are endophytic bacterial communities inhabiting the microplants before initiation of the in vitro culture and that some of these endophytes persist in their latent form and can also grow in the culture medium even after long-term micropropagation, thus discarding the concept of "truly axenic plants."

Patients who share transparent visit notes with others: characteristics, risks, and benefits.

Science.gov (United States)

Jackson, Sara L; Mejilla, Roanne; Darer, Jonathan D; Oster, Natalia V; Ralston, James D; Leveille, Suzanne G; Walker, Jan; Delbanco, Tom; Elmore, Joann G

2014-11-12

Inviting patients to read their primary care visit notes may improve communication and help them engage more actively in their health care. Little is known about how patients will use the opportunity to share their visit notes with family members or caregivers, or what the benefits might be. Our goal was to evaluate the characteristics of patients who reported sharing their visit notes during the course of the study, including their views on associated benefits and risks. The OpenNotes study invited patients to access their primary care providers' visit notes in Massachusetts, Pennsylvania, and Washington. Pre- and post-intervention surveys assessed patient demographics, standardized measures of patient-doctor communication, sharing of visit notes with others during the study, and specific health behaviors reflecting the potential benefits and risks of offering patients easy access to their visit notes. More than half (55.43%, 2503/4516) of the participants who reported viewing at least one visit note would like the option of letting family members or friends have their own Web access to their visit notes, and 21.70% (980/4516) reported sharing their visit notes with someone during the study year. Men, and those retired or unable to work, were significantly more likely to share visit notes, and those sharing were neither more nor less concerned about their privacy than were non-sharers. Compared to participants who did not share clinic notes, those who shared were more likely to report taking better care of themselves and taking their medications as prescribed, after adjustment for age, gender, employment status, and study site. One in five OpenNotes patients shared a visit note with someone, and those sharing Web access to their visit notes reported better adherence to self-care and medications. As health information technology systems increase patients' ability to access their medical records, facilitating access to caregivers may improve perceived health

46 CFR 403.120 - Notes to financial statements.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Notes to financial statements. 403.120 Section 403.120... ACCOUNTING SYSTEM General § 403.120 Notes to financial statements. (a) All matters that are not clearly identified in the body of the financial statements of the Association, but which may materially influence...

Note onset deviations as musical piece signatures.

Science.gov (United States)

Serrà, Joan; Özaslan, Tan Hakan; Arcos, Josep Lluis

2013-01-01

A competent interpretation of a musical composition presents several non-explicit departures from the written score. Timing variations are perhaps the most important ones: they are fundamental for expressive performance and a key ingredient for conferring a human-like quality to machine-based music renditions. However, the nature of such variations is still an open research question, with diverse theories that indicate a multi-dimensional phenomenon. In the present study, we consider event-shift timing variations and show that sequences of note onset deviations are robust and reliable predictors of the musical piece being played, irrespective of the performer. In fact, our results suggest that only a few consecutive onset deviations are already enough to identify a musical composition with statistically significant accuracy. We consider a mid-size collection of commercial recordings of classical guitar pieces and follow a quantitative approach based on the combination of standard statistical tools and machine learning techniques with the semi-automatic estimation of onset deviations. Besides the reported results, we believe that the considered materials and the methodology followed widen the testing ground for studying musical timing and could open new perspectives in related research fields.

Note onset deviations as musical piece signatures.

Directory of Open Access Journals (Sweden)

Joan Serrà

Full Text Available A competent interpretation of a musical composition presents several non-explicit departures from the written score. Timing variations are perhaps the most important ones: they are fundamental for expressive performance and a key ingredient for conferring a human-like quality to machine-based music renditions. However, the nature of such variations is still an open research question, with diverse theories that indicate a multi-dimensional phenomenon. In the present study, we consider event-shift timing variations and show that sequences of note onset deviations are robust and reliable predictors of the musical piece being played, irrespective of the performer. In fact, our results suggest that only a few consecutive onset deviations are already enough to identify a musical composition with statistically significant accuracy. We consider a mid-size collection of commercial recordings of classical guitar pieces and follow a quantitative approach based on the combination of standard statistical tools and machine learning techniques with the semi-automatic estimation of onset deviations. Besides the reported results, we believe that the considered materials and the methodology followed widen the testing ground for studying musical timing and could open new perspectives in related research fields.

29 CFR Appendix to Subpart B of... - Reporter's Notes

Science.gov (United States)

2010-07-01

... Part 18—Reporter's Notes Reporter's Introductory Note The Rules of Evidence for the United States... contained in its files, such as the revenue statistics contained in the reports submitted to it by a...

Dinámica de la brotación potencial de caña semilla micropropagada y termotratada de tres cultivares de caña de azúcar Potential germination dynamics of micropropagated and hot-water-treated seedcane of three sugarcane cultivars

Directory of Open Access Journals (Sweden)

Patricia A. Digonzelli

2006-12-01

Full Text Available Se comparó la dinámica de la brotación potencial (brotación en condiciones óptimas de humedad y temperatura de estacas uninodales de caña semilla saneada, obtenida por micropropagación y por termoterapia (inmersión en agua caliente 50ºC durante 2 h de tres cultivares (LCP 85-384, CP 65-357 y CP 48-103 y en tres edades (7, 10 y 12 meses. La semilla micropropagada e hidrotermotratada se encontraba libre de achaparramiento de la caña soca (Ratoon Stunting Disease, RSD (Leifsonia xyli subsp. xyli y escaldadura de la hoja (Xanthomonas albilineans. Las estacas se colocaron en una cámara de cría, en bandejas con arena lavada y esterilizada, a temperatura de 26 ± 2ºC, luz continua y control de humedad. El diseño experimental utilizado fue un factorial completamente aleatorizado (3x3x2, con dos repeticiones. Los factores fueron: edad (tres niveles, cultivar (tres niveles y origen de la semilla (dos niveles. Diariamente se contaron las yemas brotadas (clavo visible hasta la estabilización de la brotación. La dinámica de la brotación potencial se ajustó al modelo exponencial simple, función sigmoide simétrica, con R2 ≥ 0,97 y a partir del modelo se derivaron las variables deducidas: t50 y t90 (días al 50% y 90% de la emergencia máxima, porcentaje máximo de brotación, tasa media y tasa máxima de brotación potencial. La brotación en la semilla micropropagada presentó una mayor velocidad y una menor duración que en la termotratada. El porcentaje final varió entre el 80% y el 100%, en independencia del origen de la semilla.The potential germination dynamics (germination under optimal humidity and temperature conditions of healthy seedcane of three cultivars (LCP 85-384, CP 65-357 and CP 48-103 at three different ages (7, 10 and 12 months were compared, by studying one-joint billets of seedcane obtained through micropropagation and hot water treatment (inmersion into water heated at 50ºC, for 2 h. This micropropagated and hot

[Historical notes about scientific research in the Instituto Mexicano del Seguro Social.

Science.gov (United States)

Zárate, Arturo; Basurto-Acevedo, Lourdes

2013-01-01

Medical research in the Instituto Mexicano del Seguro Social has been considered one of the most important in this country for quality and quantity. Thanks to the work and leadership of Benito Coquet, who initiated the building of the National Medical Center in 1961, and the work of two pillars of research, Luis Castelazo and Bernardo Sepúlveda, the Institute successfully improved scientific research. In the years that followed, the Institute fostered the professionalization of research, the creation of research units in different areas of science, the incorporation of consolidated groups of researchers, the relationship with other institutions, the incorporation to the Sistema Nacional de Investigadores, the editing of a journal to expose outside the work done within the Institute, and the formation of a trust to raise funds for financing. Thanks to all that, institutional research strengthened in all lines, and it was placed first, at certain times, at the national level.

"Seeing things in my head": a synesthete's images for music and notes.

Science.gov (United States)

Mills, Carol Bergfeld; Boteler, Edith Howell; Larcombe, Glenda K

2003-01-01

We explored synesthetic experiences (photisms) elicited by music and notes for GS, a 22-year-old female college student, who is a genuine digit synesthete (Mills et al 1999 Cognitive Neuropsychology 16 181-191). After extensive interviews, we conducted five studies using synthesizer notes in different octaves and in different instrument sounds. GS described, drew, or selected her photisms from alternatives. The results were: (a) her photisms had shape, color, movement, and texture: (b) different instruments had photisms with different shapes and families of instruments had similar shapes; (c) low-pitched notes were darker and larger than high notes; (d) instrument and pitch affected each other; (e) a given note was perceived as darker after a high note than after a low note; and (f) GS's drawings of notes became more consistent with repeated trials. Theoretical observations are discussed on the basis of these results.

The STS-constructivist reform: some discordant notes | Nworgu ...

African Journals Online (AJOL)

It would appear that as many more voices join in the advocacy for STS constructivist Reform in science teaching/learning, more discordant notes are emitted. The purpose of the paper therefore, was to spotlight some of these discordant notes emitted in the course of the on-going reform advocacy. Specifically, three of such ...

26 CFR 25.2512-4 - Valuation of notes.

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 14 2010-04-01 2010-04-01 false Valuation of notes. 25.2512-4 Section 25.2512-4... GIFT TAX; GIFTS MADE AFTER DECEMBER 31, 1954 Transfers § 25.2512-4 Valuation of notes. The fair market... the property, if any, pledged or mortgaged as security is insufficient to satisfy it. ...

Remedial action programs annual meeting: Meeting notes

International Nuclear Information System (INIS)

1987-01-01

The US Department of Energy Grand Junction Projects Office was pleased to host the 1987 Remedial Action programs Annual Meeting and herein presents notes from that meeting as prepared (on relatively short notice) by participants. These notes are a summary of the information derived from the workshops, case studies, and ad hoc committee reports rather than formal proceedings. The order of the materials in this report follows the actual sequence of presentations during the annual meeting

Predictors of article impact in suicidology: the bereavement literature, a research note.

Science.gov (United States)

Andriessen, Karl; Krysinska, Karolina; Stack, Steven

2015-02-01

Citation analysis has been neglected in suicidology. The present note applies a mixed-methods approach to both test and suggest hypotheses for the variation in article impact in the bereavement literature. One hundred three articles from three core suicidology journals met the criteria for inclusion in the investigation. Citations to the articles were obtained from the Web of Science. Predictor variables included structural characteristics of the author (e.g., gender) and the article itself (e.g., years since publication). A multivariate regression analysis determined that, controlling for the other variables, the most important predictor of citations was the review article (β = .461), followed by year of publication (β = -.414), the multiauthored article (β = .302), publication in Suicide and Life-Threatening Behavior (SLTB) (β = .161), and male gender (β = .156). The 12 most cited articles were published between 1979 and 2004 in SLTB. The majority of these papers was written by males, were U.S. authors, and had more than one author. Four of the most cited articles were reviews. The study concludes that structural characteristics of articles and authors explained 41% of the variance in citations. The qualitative analysis determined that review papers, and papers on characteristics of suicide bereavement and psychological autopsies have been most frequently cited. Replication studies are needed for other subfields of suicidology. © 2014 The American Association of Suicidology.

Adult Education Research in the U.K.: An Overview of What Researchers Are Talking About.

Science.gov (United States)

Fordham, Paul

1978-01-01

Describes the growth of adult education and adult education research in Britain and outlines four government-financed educational research programs: survey of non-vocational provision, adult literacy program, new communities project, and paid educational leave. Notes the importance of international contacts in pursuing adult education research.…

26 CFR 20.2031-4 - Valuation of notes.

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 14 2010-04-01 2010-04-01 false Valuation of notes. 20.2031-4 Section 20.2031-4... ESTATE TAX; ESTATES OF DECEDENTS DYING AFTER AUGUST 16, 1954 Gross Estate § 20.2031-4 Valuation of notes... of the party or parties liable, or for other cause), and that any property pledged or mortgaged as...

Development and evaluation of 'briefing notes' as a novel knowledge translation tool to aid the implementation of sex/gender analysis in systematic reviews: a pilot study.

Science.gov (United States)

Doull, Marion; Welch, Vivian; Puil, Lorri; Runnels, Vivien; Coen, Stephanie E; Shea, Beverley; O'Neill, Jennifer; Borkhoff, Cornelia; Tudiver, Sari; Boscoe, Madeline

2014-01-01

There is increasing recognition of sex/gender differences in health and the importance of identifying differential effects of interventions for men and women. Yet, to whom the research evidence does or does not apply, with regard to sex/gender, is often insufficiently answered. This is also true for systematic reviews which synthesize results of primary studies. A lack of analysis and reporting of evidence on sex/gender raises concerns about the applicability of systematic reviews. To bridge this gap, this pilot study aimed to translate knowledge about sex/gender analysis (SGA) into a user-friendly 'briefing note' format and evaluate its potential in aiding the implementation of SGA in systematic reviews. Our Sex/Gender Methods Group used an interactive process to translate knowledge about sex/gender into briefing notes, a concise communication tool used by policy and decision makers. The briefing notes were developed in collaboration with three Cochrane Collaboration review groups (HIV/AIDS, Hypertension, and Musculoskeletal) who were also the target knowledge users of the briefing notes. Briefing note development was informed by existing systematic review checklists, literature on sex/gender, in-person and virtual meetings, and consultation with topic experts. Finally, we held a workshop for potential users to evaluate the notes. Each briefing note provides tailored guidance on considering sex/gender to reviewers who are planning or conducting systematic reviews and includes the rationale for considering sex/gender, with examples specific to each review group's focus. Review authors found that the briefing notes provided welcome guidance on implementing SGA that was clear and concise, but also identified conceptual and implementation challenges. Sex/gender briefing notes are a promising knowledge translation tool. By encouraging sex/gender analysis and equity considerations in systematic reviews, the briefing notes can assist systematic reviewers in ensuring the

Xyce parallel electronic simulator release notes.

Energy Technology Data Exchange (ETDEWEB)

Keiter, Eric R; Hoekstra, Robert John; Mei, Ting; Russo, Thomas V.; Schiek, Richard Louis; Thornquist, Heidi K.; Rankin, Eric Lamont; Coffey, Todd S; Pawlowski, Roger P; Santarelli, Keith R.

2010-05-01

The Xyce Parallel Electronic Simulator has been written to support, in a rigorous manner, the simulation needs of the Sandia National Laboratories electrical designers. Specific requirements include, among others, the ability to solve extremely large circuit problems by supporting large-scale parallel computing platforms, improved numerical performance and object-oriented code design and implementation. The Xyce release notes describe: Hardware and software requirements New features and enhancements Any defects fixed since the last release Current known defects and defect workarounds For up-to-date information not available at the time these notes were produced, please visit the Xyce web page at http://www.cs.sandia.gov/xyce.

Diagnostic pure transgastric NOTES in an intensive therapy unit patient

Directory of Open Access Journals (Sweden)

Maciej Michalik

2011-06-01

Full Text Available Natural orifice transluminal endoscopic surgery (NOTES is a natural consequence of evolution in minimally invasivesurgery. It allows one to reduce surgical trauma and the number of complications and to improve cosmetic outcomes.It also shortens the patient’s recovery time. So far there have been only nine NOTES procedures performed in Polandand around 200 NOTES interventions performed worldwide. In this paper a transgastric diagnostic NOTES procedurein a critically ill patient is described. A 60-year-old male patient with multi-organ failure (circulatory, respiratory andrenal insufficiency, with co-morbid hypothyroidism and diabetes mellitus hospitalized in the intensive therapy unit(ITU with unknown cause of his condition was qualified for transgastric diagnostic NOTES procedure. This interventionrevealed the diagnosis of metastatic neoplastic disease and allowed persistent therapy to be avoided. DiagnosticNOTES in selected patients seems to be a very useful. It provides fast diagnosis with relatively small perioperativetrauma. It is an easy procedure that requires a regular or operative endoscope, one surgeon and an endoscopic nurse.It can be done anywhere, including at the patient’s bedside. It provides specimens for histopathology and speeds updiagnostics and decision making, especially in terminally ill patients.

Army Medical Research and Materiel Command Resident Research Associateship Program

Science.gov (United States)

2018-05-01

Unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT During this reporting period, the NRC promoted research opportunities at AMRMC institutes through a... productivity of these Associates is listed in the technical report. 15. SUBJECT TERMS- Associateship program, post-doc, awards 16. SECURITY CLASSIFICATION OF...following activities in support of the subject contract: Outreach and Promotion The promotional schedule to advertise the NRC Research Associateship